LOCUS       DACBVI010000001       124481 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.001, whole genome shotgun sequence.
ACCESSION   DACBVI010000001 DACBVI010000000
VERSION     DACBVI010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 124481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 124481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..124481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.001"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            195..1766
                     /gene="garD"
                     /locus_tag="IU325_00005"
     CDS             195..1766
                     /gene="garD"
                     /locus_tag="IU325_00005"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HAP5807415.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLHKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1915..2250
                     /locus_tag="IU325_00010"
     CDS             1915..2250
                     /locus_tag="IU325_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PrlF family
                     antitoxin"
                     /protein_id="HAP5807416.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFDTQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2250..2714
                     /locus_tag="IU325_00015"
     CDS             2250..2714
                     /locus_tag="IU325_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YhaV"
                     /protein_id="HAP5807417.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH"
     gene            complement(2769..3578)
                     /locus_tag="IU325_00020"
     CDS             complement(2769..3578)
                     /locus_tag="IU325_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP5807418.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3827..5107
                     /gene="kbaZ"
                     /locus_tag="IU325_00025"
     CDS             3827..5107
                     /gene="kbaZ"
                     /locus_tag="IU325_00025"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HAP5807419.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLSAIWP
                     RIIALVVQPGVEFDHTNVIDYQPVKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5130..5603
                     /gene="agaV"
                     /locus_tag="IU325_00030"
     CDS             5130..5603
                     /gene="agaV"
                     /locus_tag="IU325_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HAP5807420.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5614..6393
                     /locus_tag="IU325_00035"
     CDS             5614..6393
                     /locus_tag="IU325_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates concomitant with their translocation across the
                     cell membrane; protein IIA transfers a phosphoryl group to
                     IIB which then transfers the phosphoryl group to the
                     sugar; IIC forms the translocation channel for the sugar
                     uptake;involved in N-acetylgalactosamine transport;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HAP5807421.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6383..7261
                     /locus_tag="IU325_00040"
     CDS             6383..7261
                     /locus_tag="IU325_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates concomitant with their translocation across the
                     cell membrane; protein IIA transfers a phosphoryl group to
                     IIB which then transfers the phosphoryl group to the
                     sugar; IID with IIC forms the translocation channel;
                     involved in N-acetylgalactosamine transport; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HAP5807422.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7279..7713
                     /locus_tag="IU325_00045"
     CDS             7279..7713
                     /locus_tag="IU325_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates concomitant with their translocation across the
                     cell membrane; protein IIA transfers a phosphoryl group to
                     IIB which then transfers the phosphoryl group to the
                     sugar; IID with IIC forms the translocation channel;
                     involved in N-acetylgalactosamine transport; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIA"
                     /protein_id="HAP5807423.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTAQL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7710..8843
                     /gene="nagA"
                     /locus_tag="IU325_00050"
     CDS             7710..8843
                     /gene="nagA"
                     /locus_tag="IU325_00050"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP5807424.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRDGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTDVTPAEAIHMASLH
                     PARMLGVDDVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            9194..10348
                     /locus_tag="IU325_00055"
     CDS             9194..10348
                     /locus_tag="IU325_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP5807425.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRVILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMVSCLAVFAPETINSQSFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESNDVITAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10361..11221
                     /gene="kbaY"
                     /locus_tag="IU325_00060"
     CDS             10361..11221
                     /gene="kbaY"
                     /locus_tag="IU325_00060"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HAP5807426.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            11388..11864
                     /gene="agaB"
                     /locus_tag="IU325_00065"
     CDS             11388..11864
                     /gene="agaB"
                     /locus_tag="IU325_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HAP5807427.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            11903..12706
                     /gene="agaC"
                     /locus_tag="IU325_00070"
     CDS             11903..12706
                     /gene="agaC"
                     /locus_tag="IU325_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HAP5807428.1"
                     /translation="MHEITLLQGLSLAALVFFLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLITTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASVYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            12696..13487
                     /gene="agaD"
                     /locus_tag="IU325_00075"
     CDS             12696..13487
                     /gene="agaD"
                     /locus_tag="IU325_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HAP5807429.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            13488..14255
                     /locus_tag="IU325_00080"
     CDS             13488..14255
                     /locus_tag="IU325_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HAP5807430.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSHLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGENLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATDRFLTAKVSTAIPASFLWLHNYFTCMIDEMCRR"
     gene            complement(14305..15168)
                     /gene="rsmI"
                     /locus_tag="IU325_00085"
     CDS             complement(14305..15168)
                     /gene="rsmI"
                     /locus_tag="IU325_00085"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP5807431.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQGE"
     gene            15233..17269
                     /locus_tag="IU325_00090"
     CDS             15233..17269
                     /locus_tag="IU325_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HAP5807432.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRIWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPAATTAAVSAPANPSAELKIYDTSSQPLSQILNQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGIAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            17227..17622
                     /locus_tag="IU325_00095"
     CDS             17227..17622
                     /locus_tag="IU325_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HAP5807433.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRITVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            17642..18232
                     /gene="diaA"
                     /locus_tag="IU325_00100"
     CDS             17642..18232
                     /gene="diaA"
                     /locus_tag="IU325_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HAP5807434.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            18242..18817
                     /gene="yraP"
                     /locus_tag="IU325_00105"
     CDS             18242..18817
                     /gene="yraP"
                     /locus_tag="IU325_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HAP5807435.1"
                     /translation="MKALSPIAVLLSALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(18939..19979)
                     /locus_tag="IU325_00110"
     CDS             complement(18939..19979)
                     /locus_tag="IU325_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP5807436.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVLLGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPLLNPAT
                     LVFMGFVLGWGVAAIRLVAGLVMVLLIATLVQKWVRETPLTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVIAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGIAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLA"
     gene            complement(20052..20687)
                     /locus_tag="IU325_00115"
     CDS             complement(20052..20687)
                     /locus_tag="IU325_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAP5807437.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            20815..21333
                     /gene="yhbO"
                     /locus_tag="IU325_00120"
     CDS             20815..21333
                     /gene="yhbO"
                     /locus_tag="IU325_00120"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HAP5807438.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(21313..21756)
                     /locus_tag="IU325_00125"
     CDS             complement(21313..21756)
                     /locus_tag="IU325_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5807439.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRNSGTEQA"
     gene            21807..22109
                     /gene="yhbQ"
                     /locus_tag="IU325_00130"
     CDS             21807..22109
                     /gene="yhbQ"
                     /locus_tag="IU325_00130"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HAP5807440.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPKIKSD"
     gene            complement(22096..22599)
                     /locus_tag="IU325_00135"
     CDS             complement(22096..22599)
                     /locus_tag="IU325_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP5807441.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(22593..23117)
                     /locus_tag="IU325_00140"
     CDS             complement(22593..23117)
                     /locus_tag="IU325_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HAP5807442.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            23326..24321
                     /locus_tag="IU325_00145"
     CDS             23326..24321
                     /locus_tag="IU325_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP5807443.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            24330..25208
                     /locus_tag="IU325_00150"
     CDS             24330..25208
                     /locus_tag="IU325_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP5807444.1"
                     /translation="MKYSLGPVLWYWPKETLEEFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            25289..26296
                     /locus_tag="IU325_00155"
     CDS             25289..26296
                     /locus_tag="IU325_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAP5807445.1"
                     /translation="MTDKTIPFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(26414..27658)
                     /locus_tag="IU325_00160"
     CDS             complement(26414..27658)
                     /locus_tag="IU325_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HAP5807446.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIMLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(27812..29701)
                     /locus_tag="IU325_00165"
     CDS             complement(27812..29701)
                     /locus_tag="IU325_00165"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH family ATP-dependent RNA helicase"
                     /protein_id="HAP5807447.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(29694..29774)
                     /gene="yrbN"
                     /locus_tag="IU325_00170"
     CDS             complement(29694..29774)
                     /gene="yrbN"
                     /locus_tag="IU325_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HAP5807448.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(29881..30765)
                     /gene="nlpI"
                     /locus_tag="IU325_00175"
     CDS             complement(29881..30765)
                     /gene="nlpI"
                     /locus_tag="IU325_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HAP5807449.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(30874..33009)
                     /gene="pnp"
                     /locus_tag="IU325_00180"
     CDS             complement(30874..33009)
                     /gene="pnp"
                     /locus_tag="IU325_00180"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP5807450.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YNGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(33256..33525)
                     /gene="rpsO"
                     /locus_tag="IU325_00185"
     CDS             complement(33256..33525)
                     /gene="rpsO"
                     /locus_tag="IU325_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP5807451.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(33674..34618)
                     /gene="truB"
                     /locus_tag="IU325_00190"
     CDS             complement(33674..34618)
                     /gene="truB"
                     /locus_tag="IU325_00190"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP5807452.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(34618..35019)
                     /gene="rbfA"
                     /locus_tag="IU325_00195"
     CDS             complement(34618..35019)
                     /gene="rbfA"
                     /locus_tag="IU325_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP5807453.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(35183..37855)
                     /gene="infB"
                     /locus_tag="IU325_00200"
     CDS             complement(35183..37855)
                     /gene="infB"
                     /locus_tag="IU325_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP5807454.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(37880..39367)
                     /gene="nusA"
                     /locus_tag="IU325_00205"
     CDS             complement(37880..39367)
                     /gene="nusA"
                     /locus_tag="IU325_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP5807455.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(39395..39847)
                     /gene="rimP"
                     /locus_tag="IU325_00210"
     CDS             complement(39395..39847)
                     /gene="rimP"
                     /locus_tag="IU325_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP5807456.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(40054..40130)
                     /locus_tag="IU325_00215"
     tRNA            complement(40054..40130)
                     /locus_tag="IU325_00215"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40094..40096),aa:Met,seq:cat)
     gene            40478..41821
                     /gene="argG"
                     /locus_tag="IU325_00220"
     CDS             40478..41821
                     /gene="argG"
                     /locus_tag="IU325_00220"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HAP5807457.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(41829..43454)
                     /locus_tag="IU325_00225"
     CDS             complement(41829..43454)
                     /locus_tag="IU325_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP5807458.1"
                     /translation="MTVFNKFVRSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQKTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQIFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENDIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(43905..43991)
                     /locus_tag="IU325_00230"
     tRNA            complement(43905..43991)
                     /locus_tag="IU325_00230"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43955..43957),aa:Leu,seq:gag)
     gene            complement(44006..44338)
                     /gene="secG"
                     /locus_tag="IU325_00235"
     CDS             complement(44006..44338)
                     /gene="secG"
                     /locus_tag="IU325_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP5807459.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(44566..45903)
                     /gene="glmM"
                     /locus_tag="IU325_00240"
     CDS             complement(44566..45903)
                     /gene="glmM"
                     /locus_tag="IU325_00240"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003905541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP5807460.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(45896..46744)
                     /gene="folP"
                     /locus_tag="IU325_00245"
     CDS             complement(45896..46744)
                     /gene="folP"
                     /locus_tag="IU325_00245"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HAP5807461.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(46834..48768)
                     /gene="ftsH"
                     /locus_tag="IU325_00250"
     CDS             complement(46834..48768)
                     /gene="ftsH"
                     /locus_tag="IU325_00250"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP5807462.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(48868..49497)
                     /gene="rlmE"
                     /locus_tag="IU325_00255"
     CDS             complement(48868..49497)
                     /gene="rlmE"
                     /locus_tag="IU325_00255"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HAP5807463.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            49623..49916
                     /gene="yhbY"
                     /locus_tag="IU325_00260"
     CDS             49623..49916
                     /gene="yhbY"
                     /locus_tag="IU325_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP5807464.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(50072..50548)
                     /gene="greA"
                     /locus_tag="IU325_00265"
     CDS             complement(50072..50548)
                     /gene="greA"
                     /locus_tag="IU325_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP5807465.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            50796..52229
                     /gene="dacB"
                     /locus_tag="IU325_00270"
     CDS             50796..52229
                     /gene="dacB"
                     /locus_tag="IU325_00270"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAP5807466.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNDPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(52269..53441)
                     /gene="cgtA"
                     /locus_tag="IU325_00275"
     CDS             complement(52269..53441)
                     /gene="cgtA"
                     /locus_tag="IU325_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HAP5807467.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKVEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(53457..54422)
                     /locus_tag="IU325_00280"
     CDS             complement(53457..54422)
                     /locus_tag="IU325_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HAP5807468.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(54549..54806)
                     /gene="rpmA"
                     /locus_tag="IU325_00285"
     CDS             complement(54549..54806)
                     /gene="rpmA"
                     /locus_tag="IU325_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP5807469.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(54827..55138)
                     /gene="rplU"
                     /locus_tag="IU325_00290"
     CDS             complement(54827..55138)
                     /gene="rplU"
                     /locus_tag="IU325_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP5807470.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            55397..56368
                     /gene="ispB"
                     /locus_tag="IU325_00295"
     CDS             55397..56368
                     /gene="ispB"
                     /locus_tag="IU325_00295"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HAP5807471.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            56596..56874
                     /gene="sfsB"
                     /locus_tag="IU325_00300"
     CDS             56596..56874
                     /gene="sfsB"
                     /locus_tag="IU325_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HAP5807472.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWIIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(56922..58181)
                     /gene="murA"
                     /locus_tag="IU325_00305"
     CDS             complement(56922..58181)
                     /gene="murA"
                     /locus_tag="IU325_00305"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP5807473.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(58236..58490)
                     /gene="ibaG"
                     /locus_tag="IU325_00310"
     CDS             complement(58236..58490)
                     /gene="ibaG"
                     /locus_tag="IU325_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015585657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HAP5807474.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(58650..58943)
                     /gene="mlaB"
                     /locus_tag="IU325_00315"
     CDS             complement(58650..58943)
                     /gene="mlaB"
                     /locus_tag="IU325_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HAP5807475.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(58943..59578)
                     /gene="mlaC"
                     /locus_tag="IU325_00320"
     CDS             complement(58943..59578)
                     /gene="mlaC"
                     /locus_tag="IU325_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HAP5807476.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(59597..60148)
                     /gene="mlaD"
                     /locus_tag="IU325_00325"
     CDS             complement(59597..60148)
                     /gene="mlaD"
                     /locus_tag="IU325_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HAP5807477.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(60153..60935)
                     /gene="mlaE"
                     /locus_tag="IU325_00330"
     CDS             complement(60153..60935)
                     /gene="mlaE"
                     /locus_tag="IU325_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HAP5807478.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(60943..61752)
                     /gene="mlaF"
                     /locus_tag="IU325_00335"
     CDS             complement(60943..61752)
                     /gene="mlaF"
                     /locus_tag="IU325_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HAP5807479.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            61962..62939
                     /locus_tag="IU325_00340"
     CDS             61962..62939
                     /locus_tag="IU325_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HAP5807480.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPPDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            62953..63939
                     /gene="kdsD"
                     /locus_tag="IU325_00345"
     CDS             62953..63939
                     /gene="kdsD"
                     /locus_tag="IU325_00345"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HAP5807481.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            63960..64526
                     /gene="kdsC"
                     /locus_tag="IU325_00350"
     CDS             63960..64526
                     /gene="kdsC"
                     /locus_tag="IU325_00350"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HAP5807482.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLLPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            64523..65098
                     /gene="lptC"
                     /locus_tag="IU325_00355"
     CDS             64523..65098
                     /gene="lptC"
                     /locus_tag="IU325_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HAP5807483.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            65067..65624
                     /gene="lptA"
                     /locus_tag="IU325_00360"
     CDS             65067..65624
                     /gene="lptA"
                     /locus_tag="IU325_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HAP5807484.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            65631..66356
                     /gene="lptB"
                     /locus_tag="IU325_00365"
     CDS             65631..66356
                     /gene="lptB"
                     /locus_tag="IU325_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HAP5807485.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            66404..67837
                     /gene="rpoN"
                     /locus_tag="IU325_00370"
     CDS             66404..67837
                     /gene="rpoN"
                     /locus_tag="IU325_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP5807486.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            67860..68147
                     /locus_tag="IU325_00375"
     CDS             67860..68147
                     /locus_tag="IU325_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HAP5807487.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            68265..68756
                     /gene="ptsN"
                     /locus_tag="IU325_00380"
     CDS             68265..68756
                     /gene="ptsN"
                     /locus_tag="IU325_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HAP5807488.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            68802..69656
                     /gene="rapZ"
                     /locus_tag="IU325_00385"
     CDS             68802..69656
                     /gene="rapZ"
                     /locus_tag="IU325_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP5807489.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            69653..69925
                     /gene="npr"
                     /locus_tag="IU325_00390"
     CDS             69653..69925
                     /gene="npr"
                     /locus_tag="IU325_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HAP5807490.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            70138..70770
                     /gene="yrbL"
                     /locus_tag="IU325_00395"
     CDS             70138..70770
                     /gene="yrbL"
                     /locus_tag="IU325_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HAP5807491.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAAQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALTIALQKDLQPRESKTLTLTSREA"
     gene            complement(70767..71495)
                     /gene="mtgA"
                     /locus_tag="IU325_00400"
     CDS             complement(70767..71495)
                     /gene="mtgA"
                     /locus_tag="IU325_00400"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HAP5807492.1"
                     /translation="MSKSRLTVFSFIRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVNMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEQA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGESFMQQHQLD"
     gene            complement(71492..72145)
                     /gene="elbB"
                     /locus_tag="IU325_00405"
     CDS             complement(71492..72145)
                     /gene="elbB"
                     /locus_tag="IU325_00405"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HAP5807493.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(72375..74711)
                     /gene="arcB"
                     /locus_tag="IU325_00410"
     CDS             complement(72375..74711)
                     /gene="arcB"
                     /locus_tag="IU325_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HAP5807494.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(74807..75736)
                     /locus_tag="IU325_00415"
     CDS             complement(74807..75736)
                     /locus_tag="IU325_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP5807495.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARERGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            76411..80871
                     /gene="gltB"
                     /locus_tag="IU325_00420"
     CDS             76411..80871
                     /gene="gltB"
                     /locus_tag="IU325_00420"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HAP5807496.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            80884..82302
                     /locus_tag="IU325_00425"
     CDS             80884..82302
                     /locus_tag="IU325_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP5807497.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TREEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHSMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            82486..83598
                     /locus_tag="IU325_00430"
     CDS             82486..83598
                     /locus_tag="IU325_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HAP5807498.1"
                     /translation="MESLSEGTTAGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSTRNLWQFKNFYICFQRIEI
                     VQTLSAQFTPTILQTVSGESCHLAQLAKSFPLPWSTYVRLLSVKKADARSFYEKETLR
                     CGWSARQLERQIATQFYERTLLSHDKSAMLQQHAPTETHILPQQAIRDPFVLEFLELK
                     DEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRRLRCLL
                     IVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAYYALAGLP
                     NTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(83658..84122)
                     /locus_tag="IU325_00435"
     CDS             complement(83658..84122)
                     /locus_tag="IU325_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HAP5807499.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQAIILKPGMFAVFMPGEPHKPGCVVGEPDEIKKVVVKVKADLMA"
     gene            complement(84119..84994)
                     /locus_tag="IU325_00440"
     CDS             complement(84119..84994)
                     /locus_tag="IU325_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP5807500.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGANAKTIFTHAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(84991..85680)
                     /locus_tag="IU325_00445"
     CDS             complement(84991..85680)
                     /locus_tag="IU325_00445"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative N-acetylmannosamine-6-phosphate
                     2-epimerase"
                     /protein_id="HAP5807501.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(85728..87218)
                     /gene="nanT"
                     /locus_tag="IU325_00450"
     CDS             complement(85728..87218)
                     /gene="nanT"
                     /locus_tag="IU325_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HAP5807502.1"
                     /translation="MSTSTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGRAKNDLVKTKS"
     gene            complement(87327..88220)
                     /gene="nanA"
                     /locus_tag="IU325_00455"
     CDS             complement(87327..88220)
                     /gene="nanA"
                     /locus_tag="IU325_00455"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HAP5807503.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(88342..89133)
                     /gene="nanR"
                     /locus_tag="IU325_00460"
     CDS             complement(88342..89133)
                     /gene="nanR"
                     /locus_tag="IU325_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HAP5807504.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            89513..90880
                     /gene="dcuC"
                     /locus_tag="IU325_00465"
     CDS             89513..90880
                     /gene="dcuC"
                     /locus_tag="IU325_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAP5807505.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKALDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(90924..91421)
                     /gene="sspB"
                     /locus_tag="IU325_00470"
     CDS             complement(90924..91421)
                     /gene="sspB"
                     /locus_tag="IU325_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HAP5807506.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(91427..92065)
                     /gene="sspA"
                     /locus_tag="IU325_00475"
     CDS             complement(91427..92065)
                     /gene="sspA"
                     /locus_tag="IU325_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HAP5807507.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(92460..92852)
                     /gene="rpsI"
                     /locus_tag="IU325_00480"
     CDS             complement(92460..92852)
                     /gene="rpsI"
                     /locus_tag="IU325_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP5807508.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(92868..93296)
                     /gene="rplM"
                     /locus_tag="IU325_00485"
     CDS             complement(92868..93296)
                     /gene="rplM"
                     /locus_tag="IU325_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP5807509.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(93515..94642)
                     /gene="zapE"
                     /locus_tag="IU325_00490"
     CDS             complement(93515..94642)
                     /gene="zapE"
                     /locus_tag="IU325_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HAP5807510.1"
                     /translation="MQSITPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQYCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPLMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            94836..95234
                     /locus_tag="IU325_00495"
     CDS             94836..95234
                     /locus_tag="IU325_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HAP5807511.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            95388..96755
                     /gene="degQ"
                     /locus_tag="IU325_00500"
     CDS             95388..96755
                     /gene="degQ"
                     /locus_tag="IU325_00500"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HAP5807512.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            96845..97912
                     /gene="degS"
                     /locus_tag="IU325_00505"
     CDS             96845..97912
                     /gene="degS"
                     /locus_tag="IU325_00505"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HAP5807513.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(97975..98913)
                     /gene="mdh"
                     /locus_tag="IU325_00510"
     CDS             complement(97975..98913)
                     /gene="mdh"
                     /locus_tag="IU325_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HAP5807514.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEKNALEGMLDTLKKDIALGEEFVNK"
     gene            99348..99818
                     /gene="argR"
                     /locus_tag="IU325_00515"
     CDS             99348..99818
                     /gene="argR"
                     /locus_tag="IU325_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HAP5807515.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            100183..100446
                     /gene="yhcN"
                     /locus_tag="IU325_00520"
     CDS             100183..100446
                     /gene="yhcN"
                     /locus_tag="IU325_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HAP5807516.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(100502..100774)
                     /locus_tag="IU325_00525"
     CDS             complement(100502..100774)
                     /locus_tag="IU325_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5807517.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(100866..102833)
                     /gene="aaeB"
                     /locus_tag="IU325_00530"
     CDS             complement(100866..102833)
                     /gene="aaeB"
                     /locus_tag="IU325_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HAP5807518.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            complement(102839..103771)
                     /gene="aaeA"
                     /locus_tag="IU325_00535"
     CDS             complement(102839..103771)
                     /gene="aaeA"
                     /locus_tag="IU325_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HAP5807519.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            complement(103779..103982)
                     /locus_tag="IU325_00540"
     CDS             complement(103779..103982)
                     /locus_tag="IU325_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HAP5807520.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            104165..105094
                     /gene="aaeR"
                     /locus_tag="IU325_00545"
     CDS             104165..105094
                     /gene="aaeR"
                     /locus_tag="IU325_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HAP5807521.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(105221..106666)
                     /gene="tldD"
                     /locus_tag="IU325_00550"
     CDS             complement(105221..106666)
                     /gene="tldD"
                     /locus_tag="IU325_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HAP5807522.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(106822..110622)
                     /locus_tag="IU325_00555"
     CDS             complement(106822..110622)
                     /locus_tag="IU325_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein"
                     /protein_id="HAP5807523.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWNQWKLLPGTEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGVADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKAGIDLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTQVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTQSSPFPTTERINFRGWPDAQIRCAECWFWGQKFGRIDSDITISGNTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(110690..112159)
                     /locus_tag="IU325_00560"
     CDS             complement(110690..112159)
                     /locus_tag="IU325_00560"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HAP5807524.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(112149..112742)
                     /gene="yhdE"
                     /locus_tag="IU325_00565"
     CDS             complement(112149..112742)
                     /gene="yhdE"
                     /locus_tag="IU325_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HAP5807525.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAVQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(112751..113239)
                     /gene="mreD"
                     /locus_tag="IU325_00570"
     CDS             complement(112751..113239)
                     /gene="mreD"
                     /locus_tag="IU325_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP5807526.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(113239..114342)
                     /gene="mreC"
                     /locus_tag="IU325_00575"
     CDS             complement(113239..114342)
                     /gene="mreC"
                     /locus_tag="IU325_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP5807527.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(114408..115451)
                     /locus_tag="IU325_00580"
     CDS             complement(114408..115451)
                     /locus_tag="IU325_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MreB/Mrl family cell shape determining protein"
                     /protein_id="HAP5807528.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(115756..117696)
                     /gene="csrD"
                     /locus_tag="IU325_00585"
     CDS             complement(115756..117696)
                     /gene="csrD"
                     /locus_tag="IU325_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HAP5807529.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDMKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFSLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            117848..118822
                     /locus_tag="IU325_00590"
     CDS             117848..118822
                     /locus_tag="IU325_00590"
                     /EC_number="1.3.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acryloyl-CoA reductase"
                     /protein_id="HAP5807530.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESCLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLAEAPKFAGAIINNQIQGRTLVKVN"
     gene            119799..120269
                     /gene="accB"
                     /locus_tag="IU325_00595"
                     /gene_synonym="fabE"
     CDS             119799..120269
                     /gene="accB"
                     /locus_tag="IU325_00595"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP5807531.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            120280..121629
                     /gene="accC"
                     /locus_tag="IU325_00600"
     CDS             120280..121629
                     /gene="accC"
                     /locus_tag="IU325_00600"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP5807532.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            121738..121980
                     /locus_tag="IU325_00605"
     CDS             121738..121980
                     /locus_tag="IU325_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF997 family protein"
                     /protein_id="HAP5807533.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            121970..123421
                     /gene="panF"
                     /locus_tag="IU325_00610"
     CDS             121970..123421
                     /gene="panF"
                     /locus_tag="IU325_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HAP5807534.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTIVLLIGVVHAAGGLSNAVETLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            123433..124314
                     /gene="prmA"
                     /locus_tag="IU325_00615"
     CDS             123433..124314
                     /gene="prmA"
                     /locus_tag="IU325_00615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP5807535.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
BASE COUNT        30274 a        33417 c        31257 g        29533 t
ORIGIN      
        1 acataaggtt gcgcgctaaa gcacagattt gcgctttacc ttaccgtgcg gcactgcaat
       61 ccctgaaatg attgacattg atcacatttc tgcgtttaaa ctcctgacat tcttatttca
      121 cccaatgaag tcatttattt ttaaatgaga ccaggtcctc attttaataa cccctggctg
      181 gagaatattg cacaatggcc aacatcgaaa tcagacaaga aacgccaact gcgttttata
      241 taaaagttca cgacacagat aatgtggcaa ttattgttaa tgataatggc ctgaaagcag
      301 gaacgcgttt tccggatggg ctggaattaa ttgaacatat tccccagggg cataaagtcg
      361 cattgctgga cattccggct aatggtgaaa ttattcgtta tggcgaagtg attggttacg
      421 ccgtgcgcgc aatcccacgc ggaagctgga tcgacgaatc aatggttgta ctaccggaag
      481 cgccgccgtt acacacgctg ccactggcaa ccaaagtccc ggcaccctta ccgccactgg
      541 aaggatacac ctttgagggc tatcgcaatg ccgatggcag cgtgggcact aaaaacctgc
      601 tcggcatcac caccagcgtc cactgtgtgg caggcgtggt ggactatgta gtaaaaatca
      661 ttgaacgcga tctgctaccg aaatacccga acgtcgatgg cgtggtgggg ctgaatcatt
      721 tgtacggttg tggcgtggcg attaacgcac cggcggcagt tgtgcctatt cgtaccattc
      781 acaatatttc gctgaaccct aactttggcg gcgaagtaat ggtgattggt ctgggttgtg
      841 aaaagttgca gcctgaacgc ctgctgactg gaacggatga tgtgcaagct attccagtag
      901 aaagcgccag cattgtcagt ttgcaggatg aaaagcatgt cggttttcag tccatggtcg
      961 aggatatttt gcaggtcgcc gaacgccatt tgcacaaact gaatcaacgg cagcgagaaa
     1021 cctgtccggc ttcagaactg gttgtcggca tgcagtgcgg cggtagcgat gcgttttctg
     1081 gcgtaacggc aaacccggcg gttgggtatg cgtctgatct actggtgcgc tgcggcgcaa
     1141 cggtgatgtt ttcagaagtc acggaagtgc gtgacgcgat ccatctgctg acaccacgcg
     1201 cagtgaacga agaggtcggt aaacggctgc tggaggagat ggagtggtac gataactatc
     1261 tcaatatggg aaaaaccgac cgcagcgcca acccttcgcc gggcaacaag aaaggcggtc
     1321 tggcaaacgt ggtggagaaa gcgctcggct ccattgctaa atcgggtaaa agtgcaattg
     1381 ttgaagtgct gtcgcctggt caacgcccga ctaaacgcgg attaatttac gccgcgacgc
     1441 cagccagcga ttttgtctgt ggcacgcaac aggtggcttc gggtatcacc gtgcaagtgt
     1501 ttacgaccgg tcgcggtacg ccgtacggcc tgatggcggt acccgtcatt aaaatggcga
     1561 cccgcaccga gctggcgaac cgctggtttg atttaatgga tattaatgcg ggcaccatcg
     1621 ctaccggcga agaaactatt gaagaggtgg gctggaagtt gttccacttt attctcgacg
     1681 tcgccagcgg gaagaagaaa accttctcag atcaatgggg actgcataac cagctggcgg
     1741 tgtttaaccc ggcaccggtg acctgatttc ttttggaacg tccctcgcaa aacatggcct
     1801 tagtgccatg tttttattgt ttaaagcccc cacgtacatt aataatgcat ttgcattacc
     1861 ttagttcaag cttataattt gagcagaaaa caggctgtaa aaggacagtg aatcatgccc
     1921 gctaatgctc gctctcacgc tgtactgacc actgaatcaa aggtcacgat acgcggacaa
     1981 acaactatcc ccgcgccagt gcgtgaggcc ttaaaactga agccaggcca ggacagcatt
     2041 cattacgaaa ttctgcctgg tgggcaagta tttatgtgcc gactgggaga tgaacaggag
     2101 gatcatacta tgaatgcatt tttgcgtttt ctggatgcag atatccagaa caacccgcaa
     2161 aaaactcgtc cattcgacac tcaacaagga aagaaacttg tcgctggcat ggacgtcaac
     2221 attgatgatg agattggcga cgacgaataa tggattttcc acaaagggtt aatggttggg
     2281 cgctatatgc tcatccctgt tttcaggaaa cctacgacgc tttagttgcc gaagtcgaga
     2341 cattaaaggg aaaagatcct gaaaattatc agagaaaagc cgccacaaag ttattggcgg
     2401 tagtccataa agtgattgag gagcatatca cggtcaatcc atcatcaccg gcattccgtc
     2461 atggcaagtc gttaggctct gggaaaaata aagactggtc acgggtaaaa tttggtgctg
     2521 gtcgttatcg tctcttcttt cgttacagcg aaaaagagaa agtcatcatt ctgggatgga
     2581 tgaacgatga aaacacgctg cgcacctacg gtaaaaaaac agatgcctat accgtattca
     2641 gcaaaatgtt aaaaagagga catcctcctg ccgactggga aaccctcacc cgagagacag
     2701 aagaagccca ttgatggtgt tcacaatgcc cgccgaacgc attcgtgttg accagcgggc
     2761 accctttctc actccccgac cagaatcact tcaaccccag cctttcgcag gccttccaga
     2821 ctatccgcag gaatgccttc atcaacaatg atcatgtcga tacgttgagt atcaatgatc
     2881 ttatgtaaac tggaacggtt gaacttactg gaatcggtga ccacgatgat ccgttccgca
     2941 acttcgcaca tccgacggtt taaacgggct tcatcttcat tatgcgtgct gacgccgcgc
     3001 tccagatcga tcgcatctac gccaagaaac agcatatcga agtggtaatt ttgcagcgat
     3061 tgttcagcct gatcgccgta aaaagattgc gactgacggc gcaaatgccc gccggtcatc
     3121 agcagctcaa cgccttccgc ttccagcaac gcattagcca cgttcatacc gttggtcatc
     3181 gcaattacgt cagtgtgctt gcgcatcaga cgagcaatct caaaagtggt ggtcccggaa
     3241 tcgaggatca cccgatgacc tggctgaatc aactcaacgg cagctttcgc aacgctgcgt
     3301 ttcattgcgg tgttcagcgc gcttttatct tccactgatg gctcgactga cggcgtcgtg
     3361 ctatcgcaga tcaacgcgcc gccataggca cgcacagcga tcccctgctt ttccagaaac
     3421 gccagatcgt tgcggatcgt cacagtagat acgccataca atgccgacag atcgttaacc
     3481 tgcacacttc cttgctggcg caggcgttga atgatctgct ctcgtcgctc actggtgcct
     3541 gtcactcgct tctcgcctga agcgtcggta ttactcatag taagtccttt cgtaaaactt
     3601 tcgtttcatt tcgttttgcc tattaacgcc tttctattaa gcaaatgcaa gcccacctta
     3661 cccattggcg caagctactc tcgtttcagt ggctttcatt atgtttcttt tgtgaatcag
     3721 atcagaaaac cattatcttt cgttttattt ttatctcacc atggcgcagt atcaactgaa
     3781 acaaaacgaa agattaatat cgcagtaatc tgaactggag aggaaagtga aacatctgac
     3841 agaaatggtg agacagcaca aagcgggcaa aacaaatgga atttatgccg tttgttccgc
     3901 acatccgctg gtgctggagg ctgcaatccg ctacgccagt gcaaaccaaa cgccgttact
     3961 gattgaagca acctccaatc aggtagacca gttcggcggc tataccggaa tgacgcccgc
     4021 cgattttcgc ggctttgttt gtcagctcgc cgactcgttg aatttcccgc aggatgcgtt
     4081 gattctgggt ggtgaccatc tgggtccaaa ccgctggcaa aacctgccag ccgctcaggc
     4141 aatggccaat gccgatgatt tgattaaaag ctacgttgcg gcaggattca aaaaaatcca
     4201 ccttgattgc agcatgtcct gtcaggacga tccaattccc ttaactgatg acatcgtggc
     4261 tgaacgcgcc gcccgtctgg cgaaagtggc ggaagaaacc tgtcgcgaac actttggcga
     4321 agccgatctg gagtatgtca ttggtaccga agtgccggta cctggcggcg cgcatgaaac
     4381 cttaagcgag ctggcggtca ccacgccgga tgccgcccgc gccacgctgg aagcccatcg
     4441 tcacgccttt gaaaagcagg gtctgagtgc catctggcca cgcatcattg ctctggtggt
     4501 tcaacccggc gtcgaattcg atcacaccaa cgttattgat tatcagcccg tcaaagcgac
     4561 cgccttaagc cagatggtcg aaaactacga aacgctgatt ttcgaagcgc actctaccga
     4621 ttatcaaacg ccacaatcgt tgcgccagct ggtgattgac cactttgcca tcctgaaagt
     4681 tggcccggcg ctgaccttcg ccctgcgtga agctctgttc tctctggcgg cgattgaaga
     4741 agaactggta ccagcaaaag cctgttctgg tctgcgtcag gtgctggaaa acgtgatgct
     4801 cgaccgcccg gaatactggc aaagccacta ccacggtgac ggcaacgcac gtcgcctggc
     4861 gcgtggttac agctactcag atcgcgtgcg ctattactgg ccggacagcc agattgatga
     4921 cgctttcgct catctggttc gtaatctggc ggattcacca attccgctac cgctcatcag
     4981 ccagtatttg ccgctgcaat acgtgaaagt tcgctccggc gagctgcagc caacaccacg
     5041 ggaactcatt atcaaccata ttcaggacat cctggcgcag taccacacag cctgtgaagg
     5101 ccaataagca aaacaaagag gaacacgcta tgccaaatat tgttttaagc cggattgatg
     5161 agcgtttgat tcacggtcag gtcggcgttc agtgggtcgg atttgcgggg gcaaatctgg
     5221 tgctggtagc caacgatgag gttgccgaag atccggtaca acaaaacctg atggaaatgg
     5281 tactggcaga agggatcgcc gtacgtttct ggacgctgca aaaagttatc gacaacatcc
     5341 accgcgccgc cgatcgacag aaaatcctgc tggtttgtaa aacacctgcc gatttcctga
     5401 cgctggtgaa aggtggcgtt ccggtgaatc gcattaacgt tggcaatatg cactacgcca
     5461 atggcaagca acaaatcgcc aaaacggttt ctgtggatgc gggcgatatc gcagcattta
     5521 acgacctgaa agccgctggg gtggaatgct tcgttcaggg cgtcccgaca gagcctgctg
     5581 tggacctctt taaattactt tgagggattc atcatggaaa tcagtctgtt gcaggcattc
     5641 gcgttgggca ttatcgcctt tatcgctggc ctggatatgt ttaacggctt aacccatatg
     5701 caccgcccgg tggtcctcgg cccgttagtc gggctggtac ttggcgatct gcataccggt
     5761 attttaaccg gcggtacgct ggaactggtg tggatggggc tggccccgct ggcaggcgca
     5821 caaccgccta acgtgattat cggtactatc gtcggcacgg cgtttgccat tactactggc
     5881 gtgaaacccg atgtcgcggt cggtgtcgcc gtacctttcg ctgtcgcagt acagatgggg
     5941 attaccttcc tgttctcggt gatgtctggc gtaatgtccc gctgcgaccg gatggcggag
     6001 aacgccgata ctcgcggcat tgaacgcgtg aactatctgg cactgctggc actcggcact
     6061 ttctattttc tctgcgcttt cctgcctatc tacttcggcg cggaacatgc aaaaaccatc
     6121 attgatgtcc tgccgcaacg attaatcgac ggcctcggcg tcgcgggcgg catcatgcca
     6181 gcaatcggtt ttgccgtgct gctgaaaatc atgatgaaaa acgtctacat cccctacttc
     6241 atcctgggct ttgttgccgc agcctggctc aagttaccgg tgctggctat tgctgccgcc
     6301 gcgctggcga tggcgctgat cgacctgctg cgtaaatctc ctgaaccgac tcaacctgcg
     6361 gcacagaaag aggaatttga agatggcatc taatcaaacc accctgccga acgtcagtga
     6421 aaacgaagaa acactgctga ctggcgtcaa tgaaaacgtg tatgaagatc agagcattgg
     6481 cgcagagctg acaaaaaaag atatcaaccg cgtcgcctgg cgttccatgc tgttacaagc
     6541 gtcttttaac tacgaacgta tgcaggcttc cggctggttg tacggtctgc tgcctgctct
     6601 gaaaaagatc cacactaata aacgcgacct ggcgcgcgcc atgaaggggc atatgggttt
     6661 cttcaatacc catccgtttc tggtgacgtt tgttatcggc attatccttg cgatggagcg
     6721 ttctaagcag gacgttaaca gtattcagag caccaaaatt gccgtcggtg cgccgctcgg
     6781 cgggattggc gatgcaatgt tctggctgac gctactgccg atttgtggcg ggattggtgc
     6841 aagcctcgcg ctacaaggct ctattcttgg cgctgtcgtc tttattgtgc tgttcaacgt
     6901 ggtacacctg gggctgcgtt ttggtctggc gcattatgct tatcgcatgg gcgtggcggc
     6961 gattccactc attaaagcga ataccaaaaa agtcggccat gctgcgtcaa tcgttgggat
     7021 gacggtaatc ggcgcgctgg tagcaaccta tgttcgttta agcaccacgc tggaaatcac
     7081 cgctggcgat gcagtggtta agttacaggc tgatgttatc gacaaactga tgcctgcctt
     7141 cttaccgctg gtctacaccc tgaccatgtt ctggctggta cgccgcggct ggagtccgct
     7201 gcgcctgatt gcagttaccg tggttctcgg tatcgtcggt aaattctgtc atttccttta
     7261 aatacaatga ggtattagat gttaagtatt attttgacag ggcatggcgg atttgccagc
     7321 ggcatggaaa aagcaatgaa gcaaatcctc ggcgagcaat cgcagtttat tgccatcgat
     7381 tttcccgaaa cctcaagcac cgcgcagctt acatcgcagc ttgaagaggc catcgcccaa
     7441 ctcgattgtg aagacggcat tgtttttctg acggatttgc tgggcggcac gccgtttcgc
     7501 gtagcttcaa cgctggcgat gcaaaaaccg ggctgtgaag tgatcaccgg caccaatttg
     7561 caactgctac tggagatggt gctggagcgc gaagggttaa gcggtgaaga gttccgtgtg
     7621 caggcgctgg agtgtggaca ccgtgggctg accagtttgg tggacgagtt aggccgctgc
     7681 catgaagaat gtccggtcga ggaaggaata tgacacacgt tctgcgcgcc agaaggctgc
     7741 tgactgaaga gggatggctc gatgaccatc agttgcgtat tgctgacggt gtaatcgcag
     7801 caatcgaacc gattccagtg ggcgtgactg aacgcgatgc ggaactgctc tgccccgctt
     7861 acatcgacac ccatgtacac ggtggtgcgg gcgttgatgt aatggatgac gcgccggatg
     7921 tgctcgacaa gctggcaatg cacaaagcac gcgaaggtgt cggcagttgg ttaccgacca
     7981 ccgtaaccgc gccgcttaat accattcatg cggcgctgaa acgtattgct caacgttgcc
     8041 aacgcgacgg acctggtgcg caagtgctgg ggagttatct cgaaggacca tacttcacgc
     8101 cgcagaataa aggcgcgcat ccgccggagc tgtttcgcga gctggaaatt gccgagctgg
     8161 atcaattgat tgccgtttct cagcacacct tgcgcgtggt agcgctggca ccggaaaaag
     8221 aaggggcatt gcaggccatc cgccatctta aacagcaaaa cgtacgggtg atgctggggc
     8281 atagcgcggc gacctggcaa caaacccgtg ccgcgtttga cgctggtgcc gacggcctgg
     8341 tgcattgcta taacgggatg acaggtttac atcaccgcga accgggaatg gttggcgcgg
     8401 gattaacgga caagcgcgcc tggctggaac tgatagccga tggtcatcat gtgcatccgg
     8461 cggcgatgtc gctgtgttgt tgctgtgcga aagagagaat cgtgctcatc accgacgcga
     8521 tgcaggcagc cgggatgccg gatggtcgct atacgttatg tggcgaagaa gtgcagatgc
     8581 acggtggcgt tgtccgtacc gcgtccggtg gactggcggg cagtacgctg tctgttgatg
     8641 cggcagtgcg caatatggtc gagttgacgg acgtaacgcc tgcggaagcc atccatatgg
     8701 cgtcgctgca tccggcgcgt atgctgggtg ttgatgatgt tctgggatcg cttaaaccgg
     8761 gcaaacgcgc cagcgtcgtt gcgctggata gcgggctgca tgtgcaacaa atctggattc
     8821 agggtcaatt agcttcgttt tgatggtttg ctcctttatt gggccttcac ttcccccgta
     8881 aggcctttct ttttctttct ttttgatctg tacagcggtg tcggatgcga cgctgacgcg
     8941 tcttatgcga cctacggtac gccgcatccg gcaatcggtg cacatagcaa cgtcatcatg
     9001 caattcttta atcttccctt gtcttttctt tcaacgatca caaatttcgt tttatttctt
     9061 ttttctccat tgaactttca gtttcttttc tatagatttt aatcaacgaa agacatcacc
     9121 aagtgaaatg aaacgaaagg taagcgaaag cgacaacgcc cgacgtcaag ttcatcagac
     9181 taaggattga gttatgccag aaaattacac ccctgctgct gccgcaaccg gtacatggac
     9241 tgaagaagag atccgccatc agcctcgcgc atggatccgt tcactcacca acatcgacgc
     9301 gctacgttcc gcgctcaata acttccttga accgttactg cgcaaagaga atctgcgggt
     9361 aatcctgacc ggagccggaa cctcggcatt tatcggtgac atcatcgcgc cgtggctcgc
     9421 cagccataca ggtaaaaact tcagcgccgt accgaccacc gatctggtca ccaatccgat
     9481 ggactacctg aatccagctc atccactgct gttgatctcc ttcggtcgat ccggcaacag
     9541 cccggaaagc gtcgccgccg tggaactggc aaatcaattt gtacccgagt gctatcacct
     9601 gccgatcacc tgcaacgaag cgggcgctct ttaccaaaac gcgatcaaca gcgacaacgc
     9661 gtttgccctg ctgatgcccg cagaaacgca cgaccgcggc tttgcgatga ccagcagcat
     9721 taccaccatg atggtcagct gcctcgcggt tttcgcacct gagacgatca acagccagag
     9781 cttccgcgac gtggcggatc gttgccaggc gatcctgacc tcactgggcg atttcagcga
     9841 aggtgtgttt ggttacgcac cgtggaaacg gatcgtttat ctcggcagcg gtggcttaca
     9901 gggcgcagca cgcgagtcgg cgctgaaagt actggaactg acggcgggta aactggcggc
     9961 cttttatgat tccccgaccg gattccgtca tggcccgaaa tcgctggtcg ataacgaaac
    10021 gctggtggtg gtatttgtct ccagccaccc ttacacccgt cagtatgatc ttgatctgct
    10081 ggctgaactt cgccgcgata accaggcgat gcgcgtaatc gctatcgccg cggaaagcaa
    10141 cgacgttatt accgccggtc cacatatcat cctgccgccg tcccgtcact ttatcgacgt
    10201 tgagcaggca ttttgcttcc tgatgtacgc ccagacgttt gcactgatgc agtcgctgca
    10261 catgggcaat acgccggata ccccatcagc cagcggcacc gttaaccgcg tggtgcaagg
    10321 cgtaatcatt catccgtggc aggcataaga ggatcgcatt atgagcatta tctccaccaa
    10381 atatctgtta caggacgccc aggccaatgg ctacgcggtg cctgctttta acattcataa
    10441 cgccgagacg atccaggcga tcctcgaagt gtgcagtgaa atgcgatcgc cggtgatcct
    10501 cgccgggacg ccggggacct ttaaacatat cgcgctggaa gagatctacg ccctgtgtag
    10561 tgcctattcc acaacctaca acatgccact ggcgctgcat ctcgaccacc acgaatcgct
    10621 ggatgatatt cgccgtaaag tccacgcagg cgtgcgcagt gcgatgatcg acggcagcca
    10681 cttcccgttt gccgagaacg taaagctggt gaaatcggtt gtcgacttct gccactcaca
    10741 ggattgcagc gtggaagcag aactgggccg cctgggcggt gttgaagatg acatgagcgt
    10801 tgacgccgaa agtgcattcc tgaccgatcc acaagaagct aaacgctttg tcgaactgac
    10861 tggcgtcgac agcctggcgg tagcgattgg tacggcgcac ggcttataca gcaaaacgcc
    10921 gaagattgat ttccagcggc tggcggaaat tcgtgaagtg gtggatgttc ctctggtgct
    10981 gcatggtgcc agcgatgtcc cggatgaatt tgtccgtcgc actattgaac ttggcgtcac
    11041 aaaagtgaac gttgccacag aattaaaaat agccttcgct ggcgcggtta aagcctggtt
    11101 tgcggaaaat ccgcagggta atgatcctcg ttattatatg cgcgtcggta tggatgcgat
    11161 gaaagaagtt gtcagaaata aaattaatgt ctgtggttca gcgaatcgaa tttcagcata
    11221 atcattattt ttttatgatt tatcctaatg tacttcccga ttaatatcgg ggagtgcctt
    11281 aatggtaaag gagataacta aaccttaata aatacatcag taaaatatcg caataataat
    11341 atatttaaaa aaattatatt attcaacttt atggtgagga ttacacaatg agcagtccaa
    11401 atattctctt aacccgtatt gataaccgtc tggttcacgg tcaggttggc gtgacctgga
    11461 catccaccat cggtgcaaat ctgctggtag tcgtggatga tgttgtcgct aacgatgata
    11521 ttcaacagaa attaatgggt attaccgcgg aaacctacgg ctttggcatt cgtttcttta
    11581 ctatcgaaaa aaccattaac gtcatcggca aagctgcacc gcatcagaag atcttcctga
    11641 tttgccgtac gccacaaacg gtacgtaaat tggtagaagg tggtattgac ctgaaagatg
    11701 tcaacgtcgg caatatgcat ttctcggaag ggaaaaagca aatcagcagt aaagtttatg
    11761 tcgatgacca ggatctcacg gacttacgtt ttattaaaca acgtggcgtg aatgttttca
    11821 ttcaggacgt ccctggcgat caaaaagaac aaatccctga ctaaatctaa aatcgcctta
    11881 atattggttt gaggtaataa aaatgcatga aataacccta cttcagggat tatccctggc
    11941 ggcgttagtt ttctttctgg ggattgattt ttggctggaa gccttatttt tattccgccc
    12001 gataatcgtt tgtaccctaa ctggcgctat tctcggtgat attcagactg gcttaattac
    12061 cggtggcctg acagagttgg ctttcgccgg attaacccct gcaggtggtg ttcagccgcc
    12121 caacccgatt atggcgggtc tgattaccac tgtcattgca tggtctacgg gcgttgatgc
    12181 caaaacggca attggtcttg gcctgccgtt tagtttgtta atgcagtacg tcattctgtt
    12241 cttctattcc gctttctcat tatttatgac caaagccgat aaatgcgcaa aagaggcgga
    12301 tacggcagcg ttttcccgac ttaactggac aacgatgctc atcgtcgctt cagtgtatgc
    12361 ggtgattgct ttcctctgta cttacctggc acaaggggcg atgcaggcgc tggtgaaagc
    12421 gatgcccgcc tggctgaccc acggctttga agtggctggc ggtattctgc ctgccgttgg
    12481 ttttggcttg ctgctgcgcg tgatgttcaa agcgcaatat atcccttacc tgatcgccgg
    12541 tttcctgttt gtttgctaca tccaggtcag caacctgttg ccggttgccg tactgggcgc
    12601 aggctttgcg gtgtatgagt ttttcaatgc gaaatcccgg cagcaagcgc aaccgcagcc
    12661 cgttgccagt aaaaatgaag aagaggacta cagcaatggg atctgaaatc agtaaaaaag
    12721 atatcacccg tctgggcttt cgttcgtcac tgctgcaagc gagctttaat tacgaaagga
    12781 tgcaggcggg cggttttacc tgggcgatgt tgccgatcct gaaaaagatt tataaggatg
    12841 acaaaccggg cctaagcgca gcgatgaaag ataacctcga atttattaat acccacccga
    12901 atctggtcgg attcctgatg gggttattaa tttcgatgga agaaaaagga gaaaaccgcg
    12961 acaccattaa aggcctcaaa gtggcactgt ttggcccaat cgccgggatt ggcgatgcga
    13021 ttttctggtt cactttgttg ccgattatgg cggggatttg ctcatcgttt gccagtcagg
    13081 gaaacctgct ggggccgatt ctatttttcg ccgtttacct gcttatcttt ttcctgcgcg
    13141 tcggctggac ccacgtcggt tattcagtcg gcgtgaaggc gatcgataaa gtgcgagaga
    13201 actcgcagat gattgcccgt tcggcaacca tcctcgggat cacggtaatc ggcgggctga
    13261 tcgcttcgta tgtgcatatt aacgtggtga catcgtttgc catcgacagt acccacagcg
    13321 tcgcactgca gcaggatttc ttcgataaag tcttcccgaa cattttaccg atggcctaca
    13381 ccctgttgat gtattacttc ctgcgggtga aaaaagcgca tccggtgctg ttaatcggcg
    13441 tgacttttgt gctctctatt gtttgttctg cattcggcat tttgtaaatg gaacgaggca
    13501 ctgcgtctgg tggtgcctct ttactaaagg aatttcatcc tgtgcaaacc cttcaacaag
    13561 ttgaaaacta tacggcgtta agtgaacgtg ccagcgaata tttattggcc gtgatccgta
    13621 gcaaaccgga tgccgtgatt tgcctggcga ccggagccac gccattactg acgtatcatt
    13681 atctggtaga aaaaatccac cagcagcagg ttgatgtcag ccatctcacc ttcgtgaagc
    13741 tcgacgaatg ggtggatctg ccattaacta tgcctggcac ctgcgaaact ttcctacaac
    13801 agcatatcgt gcagccgctg gggctacgtg aagaccagct catcagcttt cgctccgaag
    13861 agataaatga gacagagtgc gaacgggtaa cgaacctgat tgcgcgcaaa ggcggcctgg
    13921 atttatgcgt tctcggattg gggaaaaacg gtcatcttgg gctgaacgaa ccgggagaaa
    13981 acctgcaacc ggcctgccat atcagtcaac ttgatgccag aacacagcaa catgagatgt
    14041 taaaaaccgc gggccgtccc gtgactcgtg ggatcacctt aggcctgaag gatattctca
    14101 atgcccgcga agttttgtta ctggtgactg gcgaaggaaa gcaggatgcg acagatcgtt
    14161 ttctcacggc taaagtctct accgctatcc cggcttcatt tttatggctg cataactatt
    14221 tcacatgcat gattgatgag atgtgtcgtc gttaactcgc ctgctacagg cgttgcaacg
    14281 ctattcctgc tgcgacgcct gtaatcactc tccctgctgc tccagtgcat acttatacag
    14341 cgcatttttc ttcacgccgt gaatttctgc tgccagcgcc gccgcttttt tcagtggcag
    14401 ttctgcctgt agcagcgcca gcgtgcgcag ggcatcggcg ggtaagtctt cttcctgtgc
    14461 tttatggcct tcgacaatca gtaccatttc gcctttgcga cggttttcat cttctttgac
    14521 ccacgccagc agctcgccaa cgggcgcgcc gtgaatggtt tcccaggttt tggtcagctc
    14581 acgcgccaga acgacgtagc gggattcgcc taataccgca acgatatctt ccaggctatc
    14641 taacagacgg tgggtagatt cataaaaaat cagcgtgcgc ggctccgctt caatggcttt
    14701 tagcgcatca cggcggcctt ttgatttggc aggtaaaaag ccttcataac aaaaacgatc
    14761 ggatggtaaa cccgcggcgc ttaacgcagt gatagcagca cacggcccgg gtagcggcac
    14821 cacgcgaatc cccgcttcac ggcaggtacg caccagatgg tagccaggat cgttaattag
    14881 cggcgttccg gcatcggaaa ccagcgcaat gttttgcccc tcttgcagct tcgccagcag
    14941 cgtttcggct ttttgttgtt cgttatggtc gtgcagcgca aacaaccgag cattaatccc
    15001 aaaatgttgc agcaataaac cggtgtgacg cgtatcctcg gcggcaatca gatcaacggc
    15061 ctgtaatacc tccaacgcac gctgggtgat atccgccaga ttgccgattg gcgtcggtac
    15121 aatgtaaagc tggccctgag aattatccgc cgattggtgt tgtttcattg tgtcgtccgt
    15181 attgccgatt taatattgag cattgcgtaa aaaaaatatc actggataca ttatggtacc
    15241 ctcaacattt tctcgtttga aagccgcgcg ttgtctgcct gttgttctgg cagccctgat
    15301 tttcgccggt tgtggcaccc atactcccga tcagtccact gcttatatgc agggcacggc
    15361 gcaggctgat tctgcctttt acctgcaaca gatgcagcaa agttctgatg ataccaggat
    15421 caactggcaa ttactcgcca ttcgtgcact ggtgaaagaa ggtaaaaccg gacaggcggt
    15481 ggagttgttt aaccaactac cgcaagaact gaacgattct cagcgtcgcg agaaaacact
    15541 gctggcggca gagattaaac tggcgcagaa agattttgct ggcgcgcaaa acttgctggc
    15601 gaaaatcaca cctgcagatt tagaacaaaa ccagcaagcg cgttactggc aggcaaaaat
    15661 cgatgccagc caggggcgtc cttccattga tttactgcgc gcgttaattg ctcaggaacc
    15721 gctgctcggc gcgaaagaaa agcagcagaa tattgacgcc acctggcagg cgctctcctc
    15781 catgactcag gaacaggcga atacgctggt gatcaatgcc gacgaaaata ttctgcaagg
    15841 ctggttggat ctgcagcgca tctggtttga taaccgtaac gatcccgaca tgatgaaagc
    15901 cgggatcgcc gactggcaga aacgttatcc gaacaacccg ggcgcgaaaa tgctgccaac
    15961 gcagttggtt aacgtaaaag cgtttaaacc cgcatcgacc aacaaaatcg ccctgctgtt
    16021 gccactgaat ggccaggcag cggtatttgg tcgcactatt cagcaaggct ttgaagcggc
    16081 gaaaaatatc ggcactcagc cagtggcggc tcaggtagct gccgcacctg ccgcagacgt
    16141 agcagaacaa cctcagccgc aaaccgtgga tggcgttgcc agcccggcac aagcctcggt
    16201 tagcgatctg accggtgaac aacctgcggc ccagccggtg cctgtaagcg ccccggcggc
    16261 aaccaccgca gcggtaagcg cacccgcgaa tccatccgca gagctgaaaa tctacgatac
    16321 atcatcacaa ccacttagcc agatcctcaa ccaggttcaa caggatggcg cgagtattgt
    16381 ggtcggtccg ttgctgaaaa ataacgttga agagttgctg aagagcaaca ccccgctgaa
    16441 cgtgctggca ctgaaccagc cggagaatat cgaaaaccgc gtcaatattt gttacttcgc
    16501 gctttcaccg gaagacgaag cgcgcgatgc agcgcgtcat attcgtgacc agggtaaaca
    16561 agcgccgctg gtgctgatcc cacgcagttc attgggcgat cgcgtagcca atgcgtttgc
    16621 gcaagagtgg cagaaactgg gtggcggcac cgttctgcaa caaaaatttg gttccaccag
    16681 cgaattacgc gcgggtgtta acggcggttc tggtattgct ttaacgggta gcccgattac
    16741 tcccagagcg acaaccgact ccggcatgac gaccaacaat ccaacgctgc aaaccacgcc
    16801 aaccgatgac cagttcacca ataatggcgg tcgtgtcgat gcggtgtaca ttgtggcaac
    16861 gccgggtgaa atcgctttta tcaaaccgat gatcgccatg cgtaacggta gccagagcgg
    16921 tgcaacgctg tacgccagtt cccgcagcgc acaaggcatc gcaggcccgg atttccgtct
    16981 ggagatggaa ggcttgcagt acagcgaaat cccgatgctg gcaggcggta atctgccgtt
    17041 aatgcagcag gcgctcagtg cggtgaacaa cgattattcg ctggctcgca tgtatgcgat
    17101 gggcgtcgat gcctggtcgt tggcaaatca tttctcacaa atgcgccagg ttcagggttt
    17161 tgaaatcaac ggtaataccg gaagtctgac ggctaacccg gattgcgtga ttaacaggaa
    17221 gttatcatgg ctacagtacc aacaaggtca ggtagtcccc gccagttaac caccaaacag
    17281 accggcgatg cgtgggaagc acaagcgcgt cgctggctgg aaggcaaagg actgcggttt
    17341 atcgccgcta acgtgaacga gcgtggcggc gagatcgatc tgataatgcg tgaaggccgg
    17401 atcaccgttt ttgtcgaggt gcgttaccgc cgctctgcgc tttatggcgg cgcggcagcc
    17461 agtgtgaccc gcagcaaaca acacaaatta ttacagactg cccgcttgtg gctcgcgcgt
    17521 cataatggga gttttgatac tgtggattgc cggttcgatg tggtagcctt caccgggaat
    17581 gaggttgagt ggattaagga tgcctttaat gaccactcat aattaaggtt taaggattag
    17641 cgtgcaagaa agaattaaag cttgcttcac tgaaagcatt caaactcaaa ttgcggcggc
    17701 agaggcgctt ccggatgcca tctcccgtgc agccatgacg ctggttcagt ctctgctcaa
    17761 tggcaacaaa atcctctgtt gtggtaatgg aacttccgct gccaatgcac agcattttgc
    17821 tgccagcatg atcaaccgtt tcgaaacgga gcggcccagc ttacctgcca ttgcactaaa
    17881 tactgataat gttgtcttaa cggcgattgc caacgatcgc ttacatgatg aagtgtatgc
    17941 aaaacaggtg cgggcgctgg gtcatgcggg agatgtattg ttagccattt ccacccgtgg
    18001 caacagccgc gatattgtaa aagcggtaga agctgccgtt acgcgtgata tgaccattgt
    18061 ggcattaacc ggctatgacg gcggtgaact tgcaggtttg ttagggccac aggatgtgga
    18121 gatccgcatt ccttcgcatc gtagtgctcg cattcaggaa atgcatatgc tgacggtaaa
    18181 ttgcctgtgc gatctgatcg ataacacgct tttccctcac caggatgttt aaggagaata
    18241 catgaaggca ttatcgccaa tcgcagtcct tctttccgcg ctgctgttgc aaggttgtgt
    18301 tgccgctgcc gtagtgggta ccgctgctgt aggtaccaaa gccgcaactg acccacgcag
    18361 tgtcggcacc caggtggacg atggtaccct ggaagtgcgc gtaaacagcg cattgtcgaa
    18421 agacgaacag attaagaaag aaacccgtat taatgtcacg gcgtatcagg gcaaagtgct
    18481 gctggttggg cagtcaccaa acgctgaact ttcggctcgc gctaaacaaa ttgcgatggg
    18541 cgtagacggt gccaacgaag tgtataacga gattcgtcag ggccagccga ttggtctggg
    18601 cgaagcgtct aacgatacgt ggatcaccac caaagtgcgt tcgcagctct taaccagcga
    18661 cctggtgaaa tcgtccaacg tgaaagtgac caccgaaaac ggtgaagtgt tcctgatggg
    18721 gctagtgact gaacgtgaag cgaaagcggc ggcagatatt gccagccggg taagcggcgt
    18781 gaagcgggta accacggcgt ttacgtttat taaataagcc attgatcggt cgttgaagta
    18841 aatattcaca ccttagccga cctgttcccc tcaccctaac cctctcccca aaggggagag
    18901 gggaccgtcc agtgctgtat tcaggagcat ccaacatctt aggccaacag cgccagcccg
    18961 ccgacaatca cacctgacac tgccaccatc gcccccgtca gccataaggc tttcgccggg
    19021 aacgctttac gcaacataat cagtgacggc aaactcaccg ccgggagcgt catcaacaat
    19081 gccagcgccg gagcgattcc catgcctgcc agcatcatcg tttgtacaat tggaatttct
    19141 gctgccgttg gaatgacaaa caagcatccc gctaccgcca tcgcaatcac ccacatcagg
    19201 ctgttatcga cagcaccatc agcatggggg aataaccaga cgcgagcggc acccaacacg
    19261 agtacggcaa ggatataaac cgggatcgta ctccagaaaa gcgtccatag cgccctgccc
    19321 cagcggctaa aaaatccgcc ctgcgcttcc ggtatgtcaa tttcgaccgg tgcctgcgtt
    19381 agcggtgttt cacgtaccca tttttgcacc agcgtcgcaa tcagcaacac catcaccagc
    19441 cccgcgacca gacgaatcgc cgcaaccccc cagccgagga caaaccccat aaacaccagc
    19501 gtcgccgggt ttaacaacgg attccccatc cagaatgcca gcgccccgcc catcgatacc
    19561 tgttggcgac gcattcccgc cgcgaccgga gccgcacagc aggtacacat catgcccggc
    19621 agcgaaaaca gcgttcccag cagcgtgccg cgaaagcgcg attgcccaag cgtacgcagc
    19681 aaccagtcac gcgggatcag cacctgaatc aacgacccga gcagtacgcc cagcaccgcc
    19741 gctttccata ccgcgaggaa atagatcatc gcgtaatcca acgccgcctg ccacggatta
    19801 gcatccgcct gcgcaaggat agatttaccg atactgtggg tttcggcagc cgtaaacgct
    19861 ttgccgtagt aaggttgcca tttcacatac cagagaccag caatgacaac gagaaagaaa
    19921 agcgcgggtt tccaccactg aatgggcgtt gccgcctgag atgaagactg accagtcata
    19981 gcattcccca ggaataagtt atgataattg agcgcgtgaa tattacgctc actatcaatt
    20041 cttgggaata attattcagc tcttttgcgt aattctgaag agcttaaaat cgtcacacct
    20101 tcatgctcag gccgcatcgc ctctgccagc atgacccgcg ccacgtctct tgcatcaatg
    20161 gatttccagt tacccggtaa caaacggaac agcggcgcaa aaagcgtttc gttcatccgc
    20221 tgtttgctac gatcgcccag caacatcgaa gggcgagcaa tggtcaattt cggccagttc
    20281 tgggcgatta atgcttcttc catctccccc ttgacgcggt tatagaaaaa cggcgagtgg
    20341 gcattggcac ccatagcact gactaccagc atatgctgcg cgcccagtcg ccgcccggtt
    20401 aatgcggtat ccactaccag cgtgtaatcg gcatgaataa acgcctcttt gctccccgct
    20461 tctcgccgcg tggtgccgag acaacaaaac acaatgtcga tgggatcggt gacctgcgcc
    20521 agcgcgtcgg tcagttgcgg atcatgggga ttaaacaccc cgggcatatc gcccaacgga
    20581 cgtcgcgtcg gcgcggctat ggcgttaact ttcggttcgt taatcaacat ccgcagcagg
    20641 tgaccgccca ccagccccgt tgcgcctgta atcagtacct gactcatctt cgctccttta
    20701 cagaattgtc tgccttgcgc tccccggctc atgcaccagg cttaatagac catgaggtaa
    20761 ttatccccga ttgtgggaaa ttcgcctcat ccaatgcaac aacgcggagg aagcatgagt
    20821 aagaaaattg ccgttttaat cactgatgaa tttgaggatt cagaatttac ttcacccgca
    20881 gacgagttcc gtaaagccgg acacgaagtg attaccattg aaaagcaagc gggtaaaacg
    20941 gtgaaaggca aaaaaggaga agccagtgtg accatcgata aatccatcga tgaagtgacg
    21001 ccagcggagt ttgatgccct gctgctacca ggcggtcatt caccggatta tctgcgtggc
    21061 gacaaccgtt ttgtcacttt cacccgtgat tttgtgaata gcggcaaacc tgttttcgcc
    21121 atctgtcacg gtccgcagtt gctgatcagc gccgatgtga ttcgggggcg caaactgacc
    21181 gcagttaaac cgatcattat tgatgttaaa aacgcgggcg cggaatttta cgatcaggaa
    21241 gtcgtcgtcg ataaagatca gctggttacc agccgcacac cggacgatct gccagcgttt
    21301 aaccgcgagg cattacgcct gctcggtgcc tgagttacgt agccagatca tttttttacc
    21361 aaagcccagc gtgttgtcgg tgaatttgat ttcatcgagc cggatttccc acaccggggc
    21421 cgacagcatt ctggcaaccg gaaagcgtcg attgtacgcc tggcgtgcga ggtcgctttc
    21481 ctcaccttcc agtctgcgga tctcaccttt aaactgcacg ccgcgaatta acgctaccgt
    21541 tttcggctga ccgtttaccg ttccagcaac tgccgcctgc ggcccactca tctgcgcgtg
    21601 gcgcgttttt tcttccgtca aaatgtagaa ggcaactttc tgcgcatcaa aaagataaaa
    21661 ggcattggcg caccacaatt ccccttcctg ctgcacacac caggtgacaa catgttgttt
    21721 tgccaaccaa cggctgatgg tgatgagtgt ttccattgct gttctctttt atactgtggg
    21781 cctgacctta acatgcgacg cagacgatga caccctggtt tctttacttg atccgtaccg
    21841 ccgacaataa gctttatacc gggatcacca cagatgtcga acgccgctat cagcagcacc
    21901 aaagcggcaa aggggcgaaa gcactgcgcg ggaaaggaga actaacgctg gcgttttccg
    21961 cgccagtggg cgatcgttcg ctggcactac gggcggaata tcgcgttaag caactgacca
    22021 aacggcaaaa agagcgtctg gtagcggaag gcgcagggtt tgcagaactg ttgagcagcc
    22081 tgcaaacccc aaagattaaa agcgattgaa gtgctcgtga tactcaacca gaccggttac
    22141 gccgttcagc gcgtcatccg ctaaacgatg tacctggaag gcgctttcag tgcccggcca
    22201 gcggcaacgc agatcgtgat gcgccgccag ctcaaagccg aaacgactgt acagcgccgg
    22261 atcgcccagc gtcaccactg cggcatagcc gaactcatta agcgaatcga gtccttcata
    22321 gaccagttgg cgtgccagcc cctgtccacg gtatttttca tcaaccgcca acggtgccat
    22381 gccgacccac tgcaggtctt cgccctgcac atcaaccgga ctaaatgcca catagccaat
    22441 gacctgacct tcgtcatctg tcgccaccag ccccagcgtc agaaagccat cttcacgcag
    22501 atcgtgaacc agcttcgctt ccgcatcact ttcgaatgaa cgacgcagca gggcatcaat
    22561 acccggcgca tcaatgggaa tttctactcg aattagcatg gttcacctac cgatgtctgt
    22621 ttggtttcag gcgcggtttt cattcccgcc tcaacaaaat ccgccagttg cagcagcatc
    22681 atgcgtaagg ctttcggcat ttgctccagt tcaatggcgt ccatcaggtt tttcacatac
    22741 agccccagct ccgtatcgcc ttcaatcacc agccgacgct ggaagaagag cgtatccgga
    22801 tcttgtttac gcgccgcaat catcagcaga tcgctggcgt cggcactaaa actcacatca
    22861 gcttgcgcgt tctggctaac gaccagtttg ccattcacca ccgaggtaaa ccattgcagg
    22921 tcaatatcac gcacatgaat acttaaccag cggccttcaa gaaactccag ctcgccatca
    22981 tccagcgcct gacggaattg ccagcttaag acctgctcaa gaacctggcg ttttagcgca
    23041 aatggcgtca gttttaccgg tacactcaac agagatggcc ccaaatgcac aatacgggaa
    23101 cgcagtttat ccaacacgag ttttactccc tgtttcaaca atcatcctat tttgccatat
    23161 tagaaaaata acatagcggt ataaatcaac aattccatat gaaattgctg ctaccaccaa
    23221 tacaacttta actgccttaa atcaaaaatt gtcgcagcaa ggttaactaa aatcccagtt
    23281 cgttaacatt tttgcgtttt gatagcgcaa ccttcaggaa aaattatgga gctgctctgc
    23341 cctgccggaa atctcccggc gcttaaggcg gccatcgaaa acggcgcaga tgctgtttat
    23401 atcgggctaa aagatgatac taatgcccgt cacttcgccg gccttaactt taccgagaaa
    23461 aaattgcagg aagcggtgag ttttgtccat caacatcgcc gcaaacttca catcgcgatt
    23521 aacacttttg cgcacccgga cggttacgcc cgttggcagc gcgccgtgga tatggcggcg
    23581 cagctgggtg ccgacgcgct gatcctcgcc gacctcgcca tgctggagta cgccgccgag
    23641 cgttacccgc atattgagcg tcatgtgtca gtgcaggctt cggcgaccaa tgaagaggcg
    23701 attaactttt atcatcgcca ttttgacgtt gcacgcgtgg tgctgccgcg cgtgttgtcg
    23761 attcatcagg tgaaacaact ggcgcgggtc acgcctgtac cactggaagt ctttgctttc
    23821 ggcagcctgt gcattatgtc ggaaggccgt tgctatctgt cgtcatatct gacgggtgaa
    23881 tcgcccaaca ccgtcggtgc atgttctccg gctcgcttcg tgcgctggca gcaaacgccg
    23941 caggggctgg aatcccgcct gaacgaagtg ctgatcgacc gttatcagga cggcgaaaac
    24001 gcaggttatc cgacgctgtg taaagggcgt tatctggtgg acggcgagcg ctatcacgcg
    24061 ctggaagaac ccaccagtct caataccctg gaactgctgc cggagttaat ggcagcgaat
    24121 attgcttcgg tgaaaattga aggccgccag cgtagcccgg cgtatgtcag ccaggtggcg
    24181 aaagtctggc gtcaggctat cgaccgttgt aaggccgatc cgcaaaactt cgtaccgcaa
    24241 agcgcgtgga tggagacgct cgggtcgatg tccgaaggca cgcagaccac ccttggcgcg
    24301 tatcaccgta aatggcagtg agaaaagcaa tgaaatattc cttagggcca gtgctgtggt
    24361 actggccaaa agagacgctg gaagaatttt atcagcaggc cgccgccagc agcgccgacg
    24421 tgatttatct tggtgaagcg gtatgcagca agcgtcgggc aaccaaagtt ggcgactggc
    24481 tggagatggc aaaatcactc gccggaagtg gtaagcagat tgtgctctcc acgctggcgc
    24541 tggtgcaggc atcctctgaa ctgggcgaac tgaaacgcta tgttgagaac ggtgagtttc
    24601 tgattgaagc cagcgatctc ggcgtggtga atatgtgcgc tgaacgcaaa ctgccgttcg
    24661 tcgccgggca cgcgctgaac tgctacaacg cggtgacact gaaaatattg ctcaaacagg
    24721 gcatgatgcg ctggtgtatg ccggtggagc tttcccgcga ctggttggtg aatctgctta
    24781 atcagtgcga tgagctgggc attcgcaacc agtttgaagt ggaagtcctg agctacggtc
    24841 atctgccgct ggcctactcc gcccgctgct ttaccgcgcg ttcggaagac cgcccgaaag
    24901 atgagtgtga aacctgctgc attaagtatc cgaacgggcg caacgtgcta tcgcaggaaa
    24961 accaacaagt gtttgtactc aatggcattc agaccatgag cggctacgtt tacaacctcg
    25021 gtaacgagct gacatccatg caggggctgg tcgatgtggt ccgcctgtca ccgcagggta
    25081 ctgacacttt cgcaatgctc gacgccttcc gcgccaatga aaatggcgct gcaccactgc
    25141 cgttgacggc aaacagcgat tgtaacggct actggcggcg gctggccggg ctggagctgc
    25201 aagcgtaagt atatagctca ctttgttaac aactttaact actctttaat gcagtattaa
    25261 agattaatcg gtaacaaagt gagctgttat gactgataaa accattccgt tttcgctact
    25321 cgatctggcc cccatccccg aaggttcttc agcgcgagaa gcattctccc actctctcga
    25381 tctcgcccgt ctggctgaaa agcgcggcta tcatcgctac tggctggcag aacaccacaa
    25441 tatgactggc attgccagtg ctgccacgtc ggtattgatt ggctatctgg cggcgaatac
    25501 caccacgctg catctggggt ctggcggcgt gatgttgcct aaccactcac cgttggtcat
    25561 tgccgaacag ttcggcacgc tcaatacgct ctatccgggg cgaatcgatt tggggctggg
    25621 tcgtgctccg ggtagtgacc agcgaaccat gatggctcta cgtcgtcata tgagcgggga
    25681 tattgataat ttcccccgcg atgtcgcgga gctggtggac tggtttgacg cccgcgatcc
    25741 caatccgcat gtgcgcccgg taccaggcta tggcgagaaa atccccgtgt ggttgttagg
    25801 ctccagcctt tacagcgcgc aactggcggc gcagcttggt ctgccgtttg cgtttgcctc
    25861 acacttcgcg ccggatatgt tgttccaggc gctgcatctt tatcgcagta acttcaaacc
    25921 gtcggctcgg ctggaaaaac catacgcgat ggtgtgcatc aatattatcg ctgccgacag
    25981 caaccgcgac gctgaatttc tgtttacctc aatgcagcaa gcctttgtga aactacgccg
    26041 tggcgaaacc ggacaactac cgccaccgat tcaaaatatg gatcagttct ggtcaccgtc
    26101 tgagcagtat ggcgtgcagc aggcgctgag tatgtcgttg gtaggcgata aagcgaaagt
    26161 gcgtcatggc ttgcagtcga tcctgcgcga aaccgacgcc gatgagatta tggttaacgg
    26221 gcagattttc gatcaccagg cacgcctgca ttcgtttgaa ctggcgatgg atgttaagga
    26281 agagttgttg ggatagtgtg tcttaacgcg ggaagcctta tccgagatgg caacgctgtc
    26341 ctacgtagac ctgataagcg aagcgcatca ggcattgtgt aggcagcaga aatgtcggat
    26401 aaggcaccgc tgattactga tacaccggca gcaaattaaa gctcgataag atatggacca
    26461 gtgcgttgcc aacgccaaat accagaatca gcataatcat cggcttgccg ccccagacgc
    26521 ggaatttcgg gctaccaaag cgtttacgcg atgcgcgggc caacagcgcc ggaacaattg
    26581 ccgcccagat ggtagccgct aagccagcat aaccaatggc gtacaggaat ccgttaggaa
    26641 acagcaggcc acccacaaca ggcggggcaa aggtcagcaa cgctgttttc aaacggccca
    26701 tagccgagtc gtcgaaacca aacagatctg ccagatagtc aaacaaaccc agcgttacgc
    26761 caaggaacga actcgctacc gcaaagtttg agaacacgac cagcagaaga tccaggctac
    26821 ggctgttcag cacgccgctt aatgcctgta ccagcacatc aatattacca cccttctcgg
    26881 caatgccaat aaactcagga cgagggatgt tgcccatcgt cgccagcaac cagatggtat
    26941 acagcgccag tgccatcagc gtaccgtaca ccaggcattt cacgatggtt ttcggatctt
    27001 tgccgtaata cttcatcagg cttggcacgt taccgtgata accaaacgac gccagacaga
    27061 acggcagggt catcaacaga tacggtgcat aagacgcatt gctttcggcg acgttgaaca
    27121 atgtcgcagg ctgcacatgc cccagtaggc taccgaaggt gaggaagaag gtaatgactt
    27181 tcgcccccag cacaatcgcc gtcatgcggc tgacggcttt agtgctcaac cacaccacaa
    27241 acgccaccag cagtgcaaaa ccaaaacccg ctgcccgtgc cgggacgttc aatgacatct
    27301 ctgcgaaggt atgatgcaga atcgaaccgc tggcagaaat ataggcatag gtcaggatat
    27361 aaagcacaaa ggcaatggaa atgccgttga ccacgttcca gcctttgccc agcaaatctt
    27421 tggtgatggt gtcaaagctc gaaccgattc ggtaattcag gttagcttcc agaatcatca
    27481 agccggaatg cagcatacag aaccaggtaa agatcagcgc cgccattgac cagaagaacc
    27541 acgccccgga catgaccaca ggcagagaaa acatccctgc gccaataatg gtgccgccga
    27601 tgatcaccac gccgccaagc agcgacggtg acgtttgggt ggtggttagt gttgccatga
    27661 gggcttctct ccagtgaaaa atagtgcgac tgcgttgtta tgcattgcac tgtaccagta
    27721 cacgagtaca aaagacagaa aaaaagcccc gatggtaaaa atcggggctg tatatattat
    27781 tttacagatt gtgttcgctg tttagcgatg attacgcatc accaccgaaa cgacgacgac
    27841 cggtagagtc atcacgacgc ggagcgcggc cttcacgacg ttcgccgcta aaacgacgac
    27901 catcaccacg accaccttca cggcgttcac cgctgaagtt acgaccgcct tcacgacgtt
    27961 cgccaccgaa accacgacca ccgccacgac gctcaccgcc agtatgcggc tgtgcatcgc
    28021 ccagtaactg catgttcatc ggcttgttga gaatgcgagt gcgcgtaaag tgttgcagca
    28081 cttcacccgg catacctttc ggcagttcga tggtggagtg agaagcaaac agcttgatgt
    28141 taccgatgta acggctgctg atgtcgcctt cgttagcaat cgcgccaacg atatgacgaa
    28201 cttcaacacc atcatcgcgg cccacttcaa tgcggtacag ctgcatgtca ccaacatcac
    28261 gacgttcacg acgcggacga tcttcacggt cgccacgcgg gccacggtcg ttacgatcgc
    28321 gcggaccacg gtcatcacgg tcacggaatt cacgtttcgg acgcatcggc gcatctggcg
    28381 gaacaatcag agtacgttca ccctgtgcca ttttcagcag tgcggcggcc agagtttcga
    28441 gatccagctc ttcaccttct gcagtcggct gaattttgct cagcagtgca cggtattgat
    28501 ccagatcgct gctttccagc tgctgctgta ctttagcggc gaatttttcc agacgacgtt
    28561 tgcccagcag ttctgcgttc ggcagttcaa cttccggaat ggtcagcttc atggtgcgtt
    28621 caatgttacg cagcagacga cgctcacggt tctcaacgaa cagcagcgca cggccagcac
    28681 ggcccgcacg accagtacga ccgatacggt gaacgtaaga ctcagaatcc atcgggatat
    28741 cgtagttaac taccaggctg atacgctcaa cgtccaggcc acgggccgca acgtcggtcg
    28801 caatcaggat gtccagacga ccatctttca gtcgttccag tgtctgctca cgcagcgcct
    28861 ggttcatgtc accgttcagc gcggcgctat tgtagccgtt acgctcaaga gcttctgcca
    28921 cttccagagt cgcgtttttg gtacgaacga agataatcgc cgcatcaaaa tcttccgctt
    28981 ccaggaaacg taccagtgct tcgtttttac gcatacccca aacagtccag tagctctggc
    29041 tgatgtcagg acgggtagtc acgctggact gaatgcgcac ttcctgcggc tctttcataa
    29101 agcggcgggt aatgcgacga atcgcttccg gcatggttgc agagaacaga gcggtctgat
    29161 gaccttccgg gatctgcgcc ataatagttt caacgtcttc gatgaagccc atgcgcagca
    29221 tttcgtcagc ttcatccaga accagaccgc tcagtttaga aaggtccaga gtgccacgtt
    29281 tcaggtgatc cagcagacgg cccggagtac cgacaacgat ctgcggcccc tgacgcaggg
    29341 cgcgtaattg cacgtcataa cgctggccgc cgtacagggc aaccacgttt acgccgcgca
    29401 tgtgtttaga gaaatccgtc attgcctcag caacctgtac cgccagttcg cgggtcggtg
    29461 ccagcaccag aatctgtggt gctttcagct caggatcaag attctgcagc agaggtaaag
    29521 agaatgctgc agtttttccg ctccccgtct gggccatacc cagaacgtcg cggccattca
    29581 gcagatgtgg aatacactct gcctgaattg gagatggttt ttcgtaaccc agatcgttaa
    29641 gggcttcaag gataggagcc ttcaggccca gatctgcaaa agtggtttcg aattcagcca
    29701 tgtagtacgt gtgcctcaaa attaatggcg gccagtctac ataactcatc atgaaattga
    29761 tcagcaattt tcattgaaaa gtgtgaaccg gctcaaagta ggtgtattaa cgaacaacaa
    29821 cgccctcacc cgttaaggtg atggcaatca aaaaagatta cgggctgatg tgtacgtcag
    29881 ctattgctgg tccgattctg ccaggtcatc ttggtcctgg cccaggagcg ataattccaa
    29941 caatgcgtat cggtgctcaa caaagttatg aacgttgttg gcaaccgcca gtttgaacag
    30001 tgccgtggcg ctgtccaaat cccccagact taggtagtac ttacctaaat agaagttggt
    30061 ttcactgaga tgctcagcga gcgaggtgtt atccgttgcg tccgccttga gcctttccat
    30121 taacgtttgt tcgctaatgt tgcccaggta gaactcgaca atgttccatc cccactgttc
    30181 cttatccgat ttttcgaagt gctgtttcaa cacttcttta gcctgcttct catcgagctt
    30241 ctgctcggcg agataaagcc acagactacg gaaaggatca ttgggatcgt cttgataaaa
    30301 cgccagcaga tcatcttgcg ctaacttgtc acgaccgccg taatataatg cgatcccgcg
    30361 attcaagtgc gcgtagttgt aagttggatc aagctcaagt acagaatcaa acgcttcata
    30421 ggcagcatca aaattgcctg cctgcgttaa atatatgcct aagtaattga atacttcagg
    30481 catatccggt cggattgcca gcgcttgcga aaaatcgtta cgcgctaatg ccctcagacc
    30541 gagactatca tacaacactc cgcgctcata taaaagctgt gcgcgttcgt catcggttaa
    30601 agcccgactg gcaaggattt gttccatacg tgccagaatc acttcctgct gtaaagtcgg
    30661 ttgcaatggt accgcgagga cttcactttt acgccaggaa gtattactgc atcctgcaag
    30721 cgtaagtgct gtcgcaacga aacaccagcg caaaaaaggc ttcatttccc actcccgaag
    30781 accacggttg aatgaacgtc ctgttcccgg ttgctaacaa ggcgtcctgc ccggttaaaa
    30841 gccccccgcc gcagcggagg gcaaatggca accttactcg ccctgttcag cagccggagc
    30901 ttccggtgct gcagcaggtt gagactgctc agtcgcttct ttaatgctca gacggatacg
    30961 gccctggcga tcaacttcca gaactttcac cggtacttcc tgacccatct gcaggtaatc
    31021 ggtcactttc tcaacgcgtt tgtcagcgat ctgagagatg tgtaccagac cttctttacc
    31081 gccgccgatg gcaacaaatg cgccaaagtc aacgatacgg gtcactttac cattgtagac
    31141 gcggcccact tcgatttctg cagtgatctc ttcgatacga cgaatagcat gtttcgcttt
    31201 ctcgccgtcg gtcgctgcga tcttcacagt accgtcatct tcgatttcga tggtggtgcc
    31261 agtttcttcg gtcagggcac ggataacaga gccgccttta ccgataacgt ctttgatctt
    31321 gtccgggttg atcttgatgg tatggatacg cggagcgaac tcagagatat cgccacgtgg
    31381 cgcgttgatc gcctgttcca ttacgcccag gatatgcaga cgcgcacctt tagcctggtt
    31441 cagtgcaacc tgcatgatct ctttggtgat accttcaatt ttgatatcca tctgcaatgc
    31501 agaaataccg tcgcgggaac ccgcaacttt gaagtccata tcgcccaggt gatcttcgtc
    31561 gcccaaaatg tcagacagta caacgtagtt gtcgccttct ttcaccagac ccattgcgat
    31621 acccgcaaca gcagctttga tcggcacacc tgcgtccatc agcgccagag acgcgccgca
    31681 cacggaagcc atagaagaag aaccgttgga ttcggtgatt tcagacacca cacgtacggt
    31741 gtacgggaat ttgtccatat ccggcatgac tgccagcacg ccgcgcttcg ccagacgacc
    31801 gtgaccaatt tcacgacgct tcggagaacc gaccatgccg gtttcgccta cggagtacgg
    31861 agggaagttg tagtggaaca ggaaggtgtc agtacgttcg cccatcagtt catcaagaac
    31921 ctgcgcgtca cgtgcagtac ccagcgttgc ggtaaccagc gcctgagttt caccacgggt
    31981 aaacagcgca gaaccgtgag tacgcggcag cacgccagta cgcacatcca gaccacgaat
    32041 catatctttt tcacgaccgt cgatacgcgg ttcgcctgcc agtacgcggc tacgaacaac
    32101 gtttttctcg atagcgtgca gaatttcacc cagttcgttt tcgtccaggg tttcatcttc
    32161 agcaagcagc gtcgcgatgg tttcagattt gatgacatca acctgcgcat aacgctcttg
    32221 tttgtcggtg atgcggtaag catcgctcag acgagcttca gccagtgcag caacgcgcgc
    32281 gttcagcgct tcgtttaccg gctccggctg ccagtcccaa cgcggtttgc cggcttcttt
    32341 caccagttca ttgatgttct gaataacaac ctgctgttgt tcatgaccga acactactgc
    32401 gcccagcatc tggtcttcgc tcagcagttc agcttcagat tcaaccatca gtacagcggc
    32461 ttcagtaccc gcaacaacca gatccagttt gctctctttc agctcgtcct gagtcgggtt
    32521 cagtacgtac tggtcattga tgtaacctac gcgggcagca ccaatcggac cattgaacgg
    32581 aataccagac agagacagtg ctgcggaagc accaatcatc gcgacgatat ccgggttaac
    32641 ctgcgggtta acagaaacca cggtggcgat aacctgaact tcgttgacga agccttccgg
    32701 gaacagcggg cgaatcgggc ggtcaatcag acgcgcgatc agggtttcgc cttcgcttgg
    32761 gcggccttca cgacggaaga agctacccgg gatacgacca gcagcgtagg tacgctcctg
    32821 atagttaacg gtcagtggga agaagtcctg acctggtttg gcttttttct ggccaacaac
    32881 ggtaacaaat accgcggtgt catccatgct aaccataaca gcagcagtag cctgacgagc
    32941 catcatgccg gtttccagag tcacggtgtg ttgaccgtac tggaatttac gaacgatcgg
    33001 attaagcaat gtaatatcct ttctctttct tagacagtac cttacggcac tggtgttaat
    33061 acccgatctt ctgcgcatcc tcgcgactaa tgacaaccct aacccagccc aatgtgggta
    33121 aagcctctca ttagccgcgc gaacctctgc aacggaagat cattcatagc aacaatacat
    33181 tagtttccag tgaattgctg ccgtcagctt gaaaaaaggg gccactcagg cccccttttc
    33241 tgaaactcgc aagaattagc gacgcagacc caggcgctcg atgagctggg tgtaacgtgc
    33301 tacgtcttta cgtttcaggt agtcgagcag tttacgacgc tgagaaacca tgcgcagcag
    33361 accacgacgg ctgtggtgat cttttttgtg ctctgcaaag tggccctgca ggtggttgat
    33421 ctgtgcagtc agcagtgcta cctgaacttc ggtagaaccg gtgtcgtttg cgtcacgacc
    33481 aaactcagaa acgattttag ctgttgcttc agtacttaga gacattttaa aactccaaag
    33541 tatatagaat gaaaggacgc cgatctctaa ttcagcgatc ccagtgtacg ttacgcaaag
    33601 tgttaaacaa tttacgcgac gttaagcggc agtattctac tcgtagcgac ctgttatcgc
    33661 aagacggtta acattacgcc gggtattcaa ccaccaggcg acgaggcgcg acgcggcctt
    33721 catcgtcaat ttcgcccata ccgataaact tgccgttctc accttctgta acgcgaacca
    33781 gtccttccag cggcgcaccg gatgtacgaa ccgggttacc atttttgaag taaacagaag
    33841 acgttaacgg aagattcacc accgggtagt ccgaagctgg actgtccatt ggcatcagta
    33901 atggatcaag taactccgcg gctggaatat cctgctgttc ggcttgttca accagttcac
    33961 gcagatgttc cagggtcacc atccgttcaa ccggatattt acttaccgcc agacgacgca
    34021 ggtaaataac atgcgcgcca caaccgagtt tttcacccag gtcatcaatg atggtgcgga
    34081 tataagtgcc ttttgagcag tgaatttcca gctccagctc attgccttcg tggcgaataa
    34141 acagcaattc ataaacggta atcgggcgcg cttcacgcgg tacttcaatg ccctgacgcg
    34201 catattcgta cagttttttg ccctggtatt tcagtgctga atacatcgat gggatctgtt
    34261 cgatatcgcc acggaaagta tccagtgccg ccgccagttg ctctgcgcta aaggttaccg
    34321 gacgttcttc aacgatttgt ccatcggcgt cagacgtatc ggtacgctgt ccaagacgcg
    34381 caatcacccg atagcgtttg tcggagtcca gcagatactg ggaaaacttc gtcgcttccc
    34441 cgaggcaaat cggcaacatg ccggtcgcca gcgggtccag cgcaccggta tgcccggcac
    34501 ggttggcgtt ataaatccgt ttcacttttt gcagcgcatc gttgctggac ataccctgag
    34561 gtttatccag caacaaaacg ccgttaatgt cgcgaccgcg acgacgagga cgactcatta
    34621 gtcctccttg ctgtcgtccg ggttaacacg acgttcttcg tcatgtttga ccacgctggt
    34681 caccaggttt gacatacgca tcccttcaac cagcgagttg tcgtagaaga aggtcagttc
    34741 cggcacgata cgcaggcgca tcgctttccc cagcaggctg cggatgaaac cagaagcttc
    34801 ctgcaacgct ttgatgcccg ctttaaccgc gtcttcatct ttgtcgttga ggaacgttac
    34861 atatactttg gcatacgcca ggtcgcgaga catttcgaca ccggaaacgg tggtcatcat
    34921 gcccagacga ggatctttaa tttcacgctg caggatgaga gcgatctctt tttgcatttc
    34981 ctgcgctacg cgctgcgggc gaccaaattc tttcgccata ataaattctc ctgacaaaaa
    35041 aggggccata gccccttttt aaaaattaat ttcaggtgga agggctgttc acgttggcct
    35101 gataagacgc gcgagcgtca tatcaggcca cggctgccgg atgcggcgta aaagccttat
    35161 ccggcctaca acccagacaa ccttaagcga tggtacgttg gatttcgatg atctcgaata
    35221 cttcgatcac atcgccagtg cggacatcgt tgtagttctt aacgccgata ccacattcca
    35281 taccgttacg gacttcgtta acgtcatctt tgaagcggcg cagggactcc agctcgcctt
    35341 cgtagataac cacgttgtcg cgcagaacgc ggatcgggtt gtgacgttta accacacctt
    35401 cggtaaccat acagcctgcg atggcaccaa atttcggtga tttgaacacg tcacgaactt
    35461 ccgccagacc gataatctgt tgtttcagtt ccggagacag cataccgctc attgccgctt
    35521 tcacttcgtc gatcaggtta tagatgacgg agtagtaacg cagatccagg ctttccgctt
    35581 caattacttt acgtgcagag gcatcagcac gtacgttgaa gccaaccagg atggcgttgg
    35641 acgccgcagc cagggtagcg tcggtttcgg tgataccacc tacgccagaa ccgatgatct
    35701 tcactttaac ttcgtcagta gacagtttca gcaaggagtc ggagatagct tccacagaac
    35761 cctgtacgtc tgccttcagg acgatattca cttcgtgaac ttcgccttcg gtcatgttgg
    35821 cgaacatgtt ctcgagttta gatttctgct gacgcgccag tttaacttcg cggaatttac
    35881 cctgacgata gagtgcaact tcacgcgctt tcttctcgtc acgtacaacg gtaacttcat
    35941 cacccgcagc cggaacgccg gacaggccga ggatttccac cggaatggac ggacccgctt
    36001 ccagcacttc ctgacccagt tcgttacgca tcgcacgaac acgaccgtat tcgaagccac
    36061 acagaacgat atcgcccttg tgcagagtac cttcacgtac cagaacggta gcaaccggac
    36121 cacgaccttt atcgaggaag gattcgataa ccgcaccgct cgccatgcct ttacgtaccg
    36181 ctttcagttc cagaacttcc gcctgcagca ggatagcgtc cagcagctca tcgataccag
    36241 tacccgcttt cgcagacaca tgtacgaact ggctttcacc gccccactct tccggcagga
    36301 tgccgtactg ggagagttcg ttcttaacgc gatccggatc ggcttctggt ttatcgatct
    36361 tgttcactgc aacgaccacc ggaacctgcg ccgctttcgc gtgctggatt gcttcgatgg
    36421 tctgcggcat tacgccgtcg tcggcagcaa caaccaggac tacgatgtcc gttgcctgcg
    36481 caccacgagc acgcattgaa gtaaacgcgg cgtgccccgg ggtgtccagg aaggtgatca
    36541 tgccgttttc agtttcaacg tggtatgcac caatgtgctg ggtaatgccg cccgcttcgc
    36601 cagaggccac tttcgttgaa cgaatgtagt ccagcagaga ggttttaccg tggtcaacgt
    36661 gacccatgat ggtcacaacc ggcgcgcgcg gttcagccgc tgcacccgtg tcacggtcgc
    36721 tcattaccgc ttcttccagc tcgttttcac gacgcaggat aactttgtgg cccatctctt
    36781 cggcaaccag ctgtgcggtt tcctggtcga tgacctggtt gatggtcgcc attgcgccca
    36841 gcttcatcat cgctttgatg acctgagagc ctttaaccgc catcttgttc gccagttcgc
    36901 caacggtgat ggtttcgccg atcacaacgt cacggttaac ggcctgagca ggcttctgga
    36961 agccttgctg cagcgaagaa cctttacgtt ttccgccctt accgccacgt actgctgcgc
    37021 gtgcttcttc acgatcagct tttgattcag cgtgtttgtt gcctttcttc ggacgcgcag
    37081 ctttcgcgtt acgaccacgg ccacggccgc cttcgacttc acgatcgctt tcgtcttctg
    37141 cctggcgagc atgttgagaa gtagtgacgt gataatcgct ggaatcttca gtcggttccg
    37201 cgttatcagt ccatttgttt tcttccgcca tacgacgtgc ttcttcagca acgcgacgtg
    37261 cttcttcttc gagtttacga cgcgcttctt cttcggcttt acgcttgagc tctgcagctt
    37321 cctgctcacg gcgggctttt tcagcctggg cgtttttagt catatcgtct tgttgattac
    37381 tcactttgtc tttttccgca gcttcacgtt tcgcttgttc agcagcttca cgcttagctt
    37441 gttctgcggc ctcacgttca gctttttgtt gcgcctcgcg tttagccgat tcttctgcct
    37501 cacgacgggc ttgctcttcc gcttcacgct gcgcttgctc ttccgcagca aggcgttcag
    37561 cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg gacttcgatt tgtaccgatt
    37621 tgctttttcc accggtacca ggaatgttaa gggtgctgcg tgttttacgt tgcagcgtca
    37681 atttgtccgg gcctgaattt ttctgattca ggtggtcaat caaagtctgt ttctcttgtg
    37741 cggacacaga gtcgtcagca gacttccgga tacctgcatc agcaaattgc tgtaccaggc
    37801 gttccacgga ggtctgtcgc tctgcggcca gcgttttaat cgttacatct gtcatgctgt
    37861 tccttcctgc tacagtttat tacgcttcgt caccgaacca gcaaatatta cgggcagcca
    37921 taatcagtgc tccggctttt tcgtcggtca acccttcgat atcagccaga tcatcaatgc
    37981 cctgttcggc gagatcttcc agcgtacaaa cgccacgggc ggccagtttg aatgccaaat
    38041 cacgatctat cccttcaagg ttcagcagat cgtcagccgg tttgttatca ccgaggcttt
    38101 cttcctgggc ctgtgcaatg gtggccagtg catttttagc acgctcgcgc agtgcttcaa
    38161 cggtcggctc atcaaggcct tcgatttcca acagctcttt catcggcaca taggccagtt
    38221 cttccagcgt cgagaagcct tcttctacca gaacagtcgc gaagtcttcg tcgatgtcga
    38281 gatatttggt gaaagtgtcg atcgctgcgt gcgcttccgc ctgatgctta gcctgcaggt
    38341 cgtcaacggt catcacgttg agttcccaac cgctcagctg cgaagccaga cgcacgttct
    38401 gaccattacg gccaatcgcc tgcgccaggt taccggcttc aacggcgata tccatggtgt
    38461 gtttatcttc atccaccacg atagaagcaa cgtctgccgg tgccattgcg ttaatcacga
    38521 actgcgccgg gttatcatcc cacaggacga tatcgatacg ctcgccgccc agttcagtag
    38581 aaaccgcctg aacacgcgcg ccacgcatac ctacgcaagc acctaccgga tcgatacgtt
    38641 tatcgttggt tttcaccgcg attttcgcac gagaacccgg atcgcgagcc gctgctttaa
    38701 tttcaatcac ttcttcgccg atttccggca cttcaatgcg gaacagttcg atcagcattt
    38761 ccggcttgga acgggtgacg aacagttgcg cgccacgcgc ttccgggcga acggaataga
    38821 gcacgccacg aacgcggtcg ccaggacgga agttttcacg cggcagcata tcttcgcgca
    38881 ggatcacggc ttcagcgttg ttgcccagat ccagagagat gttgtcgcgg tttacttttt
    38941 tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa ctgatcaacc accatcgcac
    39001 gttcggcttc acgcactttc tgcacgataa cctgttttgc cgtctgggtg gtgatacggt
    39061 caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc caggttcagg ctttcatctt
    39121 cataacgtgc ggcttcaagg gtgatttcct tggtcggctg ggtgacttca tcaacaacta
    39181 accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc gatctgtacg cgaacgtcga
    39241 tttcttgttc atattttttc tttgttgctg tcgccagcgc gctttccaat gcttcgaaaa
    39301 tcttctcgcg aggtagcgcc ttttcattgg atacggcttc aactacagcc aaaatttctt
    39361 tgttcatcgc gggcttttca cctcatccag actattaaaa gtggggaacc aggttcgcct
    39421 tctggatatt actcagcgcg aacacttcat ctttaccttc gacggtaact gtgatcattt
    39481 caccgtctac cgctttgata acgccctgcc atttacgacg gttttgtacc gccatgcgga
    39541 gaaccagagt cacctcttct ccgacgaaac gggcgtagtg ttcagccgtg aacagtgggc
    39601 gatcgagacc cggtgaggag acttccaggt tataagcaac ggtgatggga tcttcaacat
    39661 ccagcacagc acttacctgg tggctcacat cagcacaatc atcaacattg atgccatctt
    39721 cactatcaat atagatgcgc agtgtggatg tgcgaccgcg aataaattcg atgccaacca
    39781 gttcaaaacc cagggcctca actggcgcag taatcatctc tgttaatttt tgctctaatg
    39841 tggacaagcc cacccccaag acataaaaaa agggcctaaa gcccagttat tctgtagtca
    39901 gataacaaaa aaccccgata aatcggggct ttatataact gaaccctata accgcaactg
    39961 cggtctggag cactttccag aaggattttt tcaaatccca ctacgaaggc cgaagtcttc
    40021 acagtatatt tgaaaaagga ctctaaggga aagtggttgc gggggccgga tttgaaccga
    40081 cgaccttcgg gttatgagcc cgacgagcta ccaggctgct ccaccccgcg cctgaaacgt
    40141 ggcaaattct actcgttttg ggtaaaaaat gcaaatactg ctgggatttg gtgtaccgag
    40201 acgggacgta aaatctgcag gcattatagt gatccatgcc acattttgtc aacgtttatt
    40261 gctaatcacg tgaatgaata tccggttcac tttcatttgt tgaatacttt tgccttctcc
    40321 tgctctccct taagcgcatt attttacaaa aaacacacta aactcttcct gtctccgata
    40381 aaagatgatt aaatgaaaac tcatttattt tgcataaaaa ttcagtgaga gcggaaatcc
    40441 aggctcatca tcagttaatt aagcagggtg ttattttatg acgacgattc tcaagcatct
    40501 cccggtaggt caacgtattg gtatcgcttt ttccggcggt ctggacacca gtgccgcact
    40561 gctgtggatg cgacaaaagg gagcggttcc ttatgcatat actgcaaacc tgggccagcc
    40621 agacgaagag gattatgatg cgatccctcg tcgtgccatg gaatacggcg cggagaacgc
    40681 acgtctgatc gactgccgca aacaactggt ggccgaaggt attgccgcta ttcagtgtgg
    40741 cgcatttcat aacaccaccg gcggcctgac ctatttcaac acgacgccgc tgggccgcgc
    40801 cgtgactggt accatgctgg ttgctgccat gaaagaagat ggcgtgaata tctggggtga
    40861 cggtagcacc tataaaggaa acgatatcga acgtttctat cgttacggtc tgctgaccaa
    40921 tgctgaactg cagatttaca aaccgtggct tgatactgac tttattgatg aactgggcgg
    40981 ccgtcatgag atgtctgaat ttatgattgc ctgcggtttc gactacaaaa tgtctgtcga
    41041 aaaagcctac tccacggact ccaacatgct tggtgcaacg catgaagcga aggatctgga
    41101 atacctcaac tccagcgtca aaatcgtcaa tccaattatg ggcgtgaagt tttgggatga
    41161 gagcgtgaaa atcccggcag aagaagtcac agtacgcttt gagcaaggtc atccggtggc
    41221 gctgaacggt aaaaccttta gcgacgacgt agaaatgatg ctggaagcta accgcatcgg
    41281 cggtcgtcac ggcctgggca tgagcgacca gattgaaaac cgtatcatcg aagcaaaaag
    41341 ccgcggcatt tatgaagccc ctgggatggc actgctgcac attgcgtatg aacgtttgtt
    41401 gaccggtatt cacaacgaag acaccattga gcagtatcac gcgcatggtc gtcagttggg
    41461 ccgtctgctg taccagggac gttggtttga ctcccaggcg ctgatgctgc gggactctct
    41521 gcaacgctgg gttgccagcc agatcactgg tgaagttact ctggagctgc gccgcgggaa
    41581 cgattattca atcctgaata ccgtctcaga gaacctgacc tacaagccag agcgtctgac
    41641 gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt atcggtcaat tgaccatgcg
    41701 taacctggat atcaccgata cccgcgagaa acttttcggt tatgccaaaa ctggcctcct
    41761 ctcctcctct gccgcttcag gcgtgccgca ggtggagaat ctggaaaata aaggccagta
    41821 attcgacatc aaccctgccc ttcaggggtg gggttacctt gcttatcctc tgtaaactcc
    41881 tttctgagca tcacataatc gaaaacatca tggtttgcat ccacaacccg tgagtctcct
    41941 ttatagtgtg caatcacttc ctccagcgtt ttcggctgtt ccggctttgt tacccccatc
    42001 cacgcattaa taaggtaatt attgtacgca gtggaaaaga tatcgttttc cgttgtacga
    42061 tcaacgccat ccccaagaac ggggctatac cagataaaca tcgggacatg atatgcctgc
    42121 tggctggcct ccctgcctcc atgaaaatag acgttcttct tcgtagggtc gcgttccagg
    42181 ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt ttaataattc aaaaatctga
    42241 cctagcaaac tatctgtgta atgaatggag ttgtcatagc aggcatcctg atcgtcctgc
    42301 ggttgaaaca cggcgctgga ttgcggatag gcgctacaag ccggttcatg gcttccattt
    42361 aaatgaagaa caatcagttt cttttgctgc gtcttttgct gtaacgcttg cgataaatgc
    42421 ggcaacaaca attcatcaaa tcctctgaca tagactgttt ccatggcgcg catggcgata
    42481 ctggtaactg ctgtaccatt ctgccgaaaa gcggattgcg agcttagcca gaaagtctga
    42541 aatcctgctt gattagccat attaataatg ttgtccgggt aattatgaat gtcatgactc
    42601 aaaacagaat cagcagttaa agaaagggga accgacagcg cagtgtaagg tgcaccgctt
    42661 attgcttgat taaacagttt gatctgtttt ctttgtgctt cgacttgcgg tgtcgtagag
    42721 cgtgtgtatc catacaaaga catattgtcg acacgtacag actcccccac aatcaacacg
    42781 taggtatcaa tacctgtatc cctgactgat aattgaaaat atggcaccgt gtttgcaatt
    42841 gagagtaatc tttgatgctc tttcgctgct aaagcaaaat aattgagatt gaaaaacggc
    42901 gtataggtag caaatcgcga cgctagtata tatggactga acgctttttt attttttgca
    42961 tctttataag caaattgaca agcggaaaat aaactgcccg aaatgacaat cagcaataat
    43021 attcctgtca cttttttcgt cggcaaggaa acatcatatt ttattattac tgccaaaaaa
    43081 agaagggata aaaaggcaaa agcacataga taagggatat acatccccag cattttgaca
    43141 acttcatccg gatcactctg cagcacacta atcgcaaatc catcattgaa agttgtacca
    43201 aaggtacacc atgaatagag gctaatactc atgtctgccg ctgtcagtaa cacaaatggt
    43261 gcggcaacaa gcaggcgaag cggaagggat gatatacgtt taacaaccaa gatggtcaga
    43321 acgaagaaca gcaatcgctg aaccatatgc gctccggaag aggcgactaa gttcgtaata
    43381 ccaaaaacaa taacacaaag atacaacaac caatgagatt taaaacttct aacaaatttg
    43441 ttgaatactg tcatccagaa atccaagttc acaaccaata taaacaatta tcaacaatgt
    43501 tcaattttat atcagacatg tttttcaact ctgatccata tcagaccgaa acactgttag
    43561 ttttttcttt ttaaatgatg cggccattgt tctcagtaat taaataacat tatcaatgcg
    43621 tctctaaaca ttacgcagaa cgtagccatc agtagttata ctgacaggag caggatggtt
    43681 tatcgagaag taaacatatc cgtaccggaa ctggagttga cacaataaag tgccaattat
    43741 gtcagcagaa gggaaaagtt tataactaag gcgtaccggc accatcgttt caaagtacca
    43801 gctacgagta aagcaactgg acgagataca gatacctgac aaccattcct cagaccagga
    43861 ccaaaacgaa aaaagacgct tttcagcgtc tcttttctga aatttggtac cgaggacggg
    43921 acttgaaccc gtaagcccta ttgggcacta ccacctcaag gtagcgtgtc taccaattcc
    43981 accacctcgg cacggatact actttttagt tcgggatatc gctggtcggc ttagccggag
    44041 cagctggctg agtttgttcg gttttcgccg gcgcactcag gttttcccat tcgctacctt
    44101 tattggtttt attgctattg atgttaccca gcaccagact gatgatgaag aataacgttg
    44161 ccagcagcgc cgtcatgcgg gtcatgaagt taccagaacc acttgaacca aacagcgtag
    44221 cggaagcgcc tgctccgaag gaggctccca tatcagcgcc tttaccttgc tgcagcatga
    44281 tcagaccaac aaggccaatt gccacaataa ggaaaactac taaaagagct tcgtacataa
    44341 tcaacctgtt ccttgcggag ttgccgcgta ccaatgcttc aaccaataaa gcgggagttt
    44401 ttaatctttc ccactgaagc gggtgtgaat actaaccaaa gcgaatgacc ttcgcaaggg
    44461 caattttatc gcattgtatc aactgcggaa aaaaacagca aaaccgatgt gttcgccgag
    44521 aaaaaaggcg acaatcgccg cctttttagc cagttatcta acgctttaaa cggcttttac
    44581 tgcatcggcg atacggtgtg caaattcagt tacctgcgct tcgtcttcac cttctaccat
    44641 cacgcgaatt aacggctcgg tgccagattt acgcagcaaa acgcgtccac ggttgcccag
    44701 cgcagcttca acctctgcgg tcacggcttt aactgactca tgctcaagcg gatcgccgct
    44761 acctgcggtg taacgcacgt taaccagaat ctgcgggaac attttcatgc cgctgcaaag
    44821 gtcgtgcagg ctcatatggt tacgcgccat cgccgccagc acctgcaagc cagcaacgat
    44881 accgtcaccg gtagtggttt tatccagcag gatcacatga ccggagttct ctgcaccgat
    44941 acgccagcct ttctcctgca ttttttccag tacgtagcgg tcccccactt tcgcgcgcgc
    45001 aaatggaata cctaactgtt tcagcgccag ttcaagcccc atgttgctca tcaatgtacc
    45061 caccgcgcca ccacgcagct ggccctgacg aagaccttca cgcgcgatga tatacatgat
    45121 ctgatcgcca tcgactttat tgccttcatg gtcaaccata atcacgcgat cgccgtcgcc
    45181 gtcgaaggca ataccgagat ccgctttttc agccagcact cgcgcctgaa gcgcgcgaac
    45241 gtcggtagcc cccacttcgg cattgatgtt cacaccgttc ggctcacaac cgatagcgat
    45301 aacattcgcc cccagttcgc gcagcacgtt cggcgcgatg tgataagtcg caccatttgc
    45361 acaatccacc acaatcttca gttcactgag gctaagttcg ttcgggaacg tggctttgca
    45421 aaactcgata tagcgacccg cagcatcaac gatacggctg gctttaccca gttctgccga
    45481 atcaacacag ctgatctctt tttccatttc cgcttcgatg gcctcttcta ctgcatccgg
    45541 tagtttggtg ccgtcgatag agaaaaattt aatgccgtta tcgtagaacg ggttatgcga
    45601 tgcagatatc acaattccgg cctctgcgcg gaaggtacgc gtcagataag ccacggccgg
    45661 tgttggcatc gggccagtga agagcgcgga aaggcccgct gccgccagac ccgcttccag
    45721 tgctgactcc agcatatagc cagaaatacg cgtgtcttta ccaataatga tcttacgaga
    45781 gccgtggcgc gccagcactt tacccgcggc ccaacccagc ttaagcacaa aatcaggggt
    45841 gatcggcgca tcccctacac gaccacgaat cccatcggta ccgaaatatt tacgattact
    45901 catagcgttt gttttccttt gcagacagag tggcttccac cacccgcatc gcttctacgg
    45961 tttctttgac gtcatgaaca cgaatgatat gcgcgccttg catagcggca atgaccgcac
    46021 aagccagact accgctcaga cgctcggacg gccccacgtt cagcagctgc ccaatcatcg
    46081 attttcgtga catacccacc aacagcggca ggttgaaatg gtgaaattca gccaggcgcg
    46141 ccagtaatga atagttatgg gagagatttt taccgaaacc gaatccgggg tcgagcaaca
    46201 atttctcttt tgcgataccc gcctgctcgc aacgtgctat ttgctcaata aaatagcgat
    46261 tcacttccgc aaagacatcg tcatacttcg gggcttcctg catggtcttt ggatttccct
    46321 gcatatgcat caggcaaacc ggcaaaccgg tttctgcagc agcctccaga gcgcctggtt
    46381 cggaaaggga gcgaatatcg ttaataatgt gagcgccaac tttcgctgac tcacggatga
    46441 cttctggttt ggatgtatcg accgagatcc agacttcgaa gcgttgagca attgcctcaa
    46501 ccacaggaat aacacgttgc aactcttctt caacgctaac ttccgccgcc cccgggcgtg
    46561 tggactcgcc accaacgtca atgatcgtcg cgccagcgtt gatcatcaga ttcgcatgtt
    46621 tcaccgcatc tatcagcgag ttatgcgtgc cgccatccga aaaggaatca ggcgtgacgt
    46681 tgaggatccc cattacgtga ggatggctaa ggtccagtga agtgccctgg gcaaagagtt
    46741 tcatggtgtt atccctggta taaattgata agaaaaaacc ccggagcacg ccccggggtt
    46801 ttcggtacaa atacagtcat ctgatgcggg aacttacttg tcgcctaact gctctgacat
    46861 ggtgttaccc gggttcggcg tacgcggttc atcaaccgga cgaggagcct ttggactgcc
    46921 attgtcgcca gaattgttag aagcgcctgg ttcttcccag cccgctggcg gacgtacatc
    46981 gcgacgtgcc atcaggtcat caatctgcgg tgcgtcgata gtctcatatt tcatgagagc
    47041 atctttcatc gcatgcagaa tatccatatt gtcggtcaga agctgacgcg cacgattata
    47101 gttacgctca atcagtgctt tcacttcctg gtcgatgata cgtgcagttt catcagacat
    47161 atgtttcgct ttcgctacgc tacggccgag gaacacttca ccttcttctt ccgcgtacag
    47221 cagtggaccc aatttctcag agaagcccca ctgagtcacc atgttacgtg ccaggttggt
    47281 cgcaacttta atatcgttgg acgcaccggt agaaacatgt tccggcccgt agatgatctc
    47341 ttctgccaga cgaccaccgt acagcgtaga aatctggctt tccagtttct gacggctggc
    47401 gctgattgcg tcgccctcag gcaagaagaa agtcacaccc agcgcacgac cgcgtgggat
    47461 aatcgtcact ttgtgcaccg gatcgtgttc cggcaccagg cgaccgataa tcgcatgacc
    47521 cgcttcgtgg taagccgtcg attctttctg cgcttccgtc atcaccatgg agcgacgttc
    47581 cgcacccatc atgattttgt ctttcgcttt ctcgaactca accatcgaca caacgcgttt
    47641 gttgccacga gcagcgaaca gtgccgcttc gttcaccagg ttcgccaggt cagcaccgga
    47701 gaaaccagga gtaccacggg caatgattgc cgcgtcgata tcgggtgcca atggtacgcg
    47761 acgcatgtga actttcagga tctgctcacg accacgaaca tctggcaagc caaccacaac
    47821 ctgacggtcg aaacggccag gacgcagcag ggccgggtca agaacgtccg gacggttagt
    47881 cgcggcgata acgatgatac cttcgttacc ttcgaagcca tccatctcaa ccagcatctg
    47941 gttcagagtc tgttcacgtt catcgtgacc accgcccaga ccagcgccac gctggcggcc
    48001 tacggcgtcg atttcatcga taaagatgat gcacggtgcc gctttcttcg cctgttcgaa
    48061 catgtcacga acacgggatg cacccacacc gacgaacatt tcgacgaagt cagaaccgga
    48121 gatagtaaag aacggaactt tcgcttcgcc tgcaatcgct ttcgccagca gcgttttacc
    48181 ggtacccgga ggaccgacca tcaggacgcc tttcgggatc ttaccgccga gtttctggaa
    48241 gcggctcggc tcgcgcagat actcaaccag ttcagcaact tcttcttttg cttcgtcgca
    48301 gcccgcaacg tcagcaaagg tcgttttgat ctgatcttcc gtcagcatgc gcgctttgct
    48361 cttaccaaac gacatggcac ctttgccacc gccgccctgc atttgacgca tgaagaagat
    48421 ccagacacca atcagcaaca gcatcgggaa ccaggagatg aagatagaag ccagcaggct
    48481 tggttcttca ggcggttcac cgacaacctt gacgttcttg gtcaacaggt tatccagtaa
    48541 tttcggatcc tgaaccggaa tgtaagtggt ataacggtta ctatctttct tggtaacgtt
    48601 gatttcacgt ccgttgatac gcgcttcacg aacctggtcg ttattgacct cttgtaggaa
    48661 ggtagagtaa tccaccttac ggccattaga ctcgctgggc ccaaagctct ggaatactga
    48721 catcagcaca acggcaatga ccagccagag tattaggttt ttcgccatgt cactcaaggg
    48781 attaacctct tattacaact gtgttaaaaa cagcgtcagg atactctata tccagcatct
    48841 ttcaaacttt cgtctgaaat ctcccggtta gggtttacgc ccggtcgcta caatatacac
    48901 ttcccgcgaa cgtgcgcgag aagagtccgg cttacgaact ttgaccttcg taaacaggga
    48961 gcgaatttcc cttagatact catcgaaacc ttcgccctgg aacaccttca ctacaaaact
    49021 gccacctggc gctaatacat cacgacacat ttctagcgcc agttccacca gatacatggc
    49081 acgggggata tccaccgccg gtgttccgct catgtttggt gccatatcgg acatgacaac
    49141 ctggactttg ctgtcgccaa cgcgctccag cagtgctttc atcaccagtt catcacgaaa
    49201 atcgccctga agaaagtcca caccaacgat aggatccata ggtaaaagat cgcaagcgat
    49261 gatgcggcct ttgccgccaa tttgggtgac cacatattgt gaccaaccac ccggcgcagc
    49321 accaaggtcg acaaccgtca ttcccggttt aaagagtttg tcactttgct gtatttcatc
    49381 aagtttaaac caggcacggg aacgtaaccc ctttttctgt gcctgttgaa catatttatc
    49441 gctaaagtgt tcctgaagcc agcggctgga gctggcagaa cgcttcttac ctgtcattta
    49501 actttcccat ggggataact catcgtaacc aattgcgtaa atttttacgc gcctatttgg
    49561 tgatatatgg gagatggcgg tagaatgacc cgttttcaat cccaacgtaa gcaaaaaata
    49621 cgatgaatct gagtactaaa caaaaacagc acctgaaagg tctggcacat ccgctcaagc
    49681 cagttgttct gcttggcagt aatggtttga ccgaaggggt gctggccgag attgaacaag
    49741 cgttagagca ccatgaactc atcaaggtga aaatcgccac cgaagatcgc gaaactaaaa
    49801 ccttgatcgt ggaagctatc gtgcgcgaaa ccggcgcctg taatgtacag gtcatcggta
    49861 aaacgctggt gctttatcgc ccaactaaag aacgtaaaat ctcgctgcca cgctaagatt
    49921 atcctaaagt tacacacatt tgctgtgtaa aacgaggggt tttccgcagg caggagagca
    49981 aaatgccacg ctctgttcgt tgataaaagg ccgcatagcg gcctttttcc tttctttaca
    50041 atacatcaac atcttgagta ttgggtaatt cttacaggta ttccacctta attacttcaa
    50101 attctacttc gccgcccggc gttttgatga ccacaacatc atcttcttct ttgccgatca
    50161 ggccacgagc aataggcgag tttacagaaa tcaggttttg tttaaagtca gcttcgtcat
    50221 cgccaacgat gcgataagtc tgttcttcgt cagaatcgag attcagcacc gttacggtag
    50281 caccaaaaat aacgcgccca ttgttgggca ttttggtgac atcaatcacc tgcgcgttcg
    50341 acagcttggc ttcgatgtct ttgatacggc cttcgcagaa gccctgctgt tcacgagctg
    50401 cgtggtattc ggcgttttct ttcaggtcgc catgctcacg cgcttccgcg atagcagcaa
    50461 tgatttcagg acggcgcaca gatttcagaa aatccagctc ttcgcgtaat ttttcagcgc
    50521 cgcgtaaggt catcggaata gcttgcattt gttatacctc ttgaatattc ctgatggggc
    50581 aagtcttcac ccaccccggc tgttcggcct gcccggcata actcctcacc gggaccagaa
    50641 gcaaaaaaat accgacccgg gtacaagtcc caggtcagct acaattcaca ttttgatagt
    50701 cattttaccc tgaagttccc gaagggtcat cgtttacttt atagggcgtt gcgccgtagt
    50761 atgacggctc gattccaggt tgttagcgcg agattatgcg attttccaga tttatcatcg
    50821 gattgaccag ctgtatagcg ttcagtgttc aggccgcaaa tgttgatgag tacattactc
    50881 aactccccgc tggtgccaac cttgccctga tggtgcaaaa agtcggcgcg tcggcccccg
    50941 ctattgatta ccacagtcag cagatggcgc tgcccgccag tacgcagaaa gttattactg
    51001 cgctggcggc gttgattcaa ctcggccccg attttcgttt taccacgacg cttgaaacca
    51061 aaggtaatgt ggaaaacggc gtacttaagg gtgacttagt ggcgcgattt ggtgccgatc
    51121 cgacgttaaa acgtcaggat attcgcaata tggtcgcgac tttgaaaaaa tctggcgtca
    51181 accaaatcga tggcaatgtg ttgatagata cctccatttt cgccagccac gataaagccc
    51241 ccggctggcc atggaatgac atgacacaat gctttagcgc tccgcctgcc gccgccatag
    51301 ttgaccgcaa ctgtttctcc gtctcgctct acagtgcccc aaaacctggt gatatggctt
    51361 ttatacgcgt ggcatcttat taccccgtta cgatgttcag ccaggtacgc accctccccc
    51421 gtggttctgc cgaagcgcaa tattgcgagc tggatgtggt gccaggcgac ctgaaccgct
    51481 ttacgctgac cggatgcctg ccacaacgtt ctgagccgct cccgttggct tttgccgtgc
    51541 aggatggagc cagctatgcc ggtgcaattc tgaaagatga gttaaaacag gcaggtatca
    51601 cctggagcgg aacactgctg cgtcagacgc aggttaacga ccccggaacg gtagttgcca
    51661 gtaaacagtc ggccccgctg cacgatctgc ttaagattat gctgaaaaag tcggacaaca
    51721 tgattgccga tacggttttc cgcatgatcg gccatgcgcg cttcaatgtg cctggaacat
    51781 ggcgggccgg gtcggacgcc gtgcgtcaga tcctgcgcca gcaagccggt gtcgatattg
    51841 gtaacaccat tattgccgat ggttcagggc tttcgcggca taacctgatt gcccccgcca
    51901 ccatgatgca ggtgctgcaa tacatagccc aacacgacaa tgaacttaac tttatctcta
    51961 tgctgccgct ggcgggctat gacggctctt tgcagtaccg tgcaggtctg catcaggcgg
    52021 gcgtggatgg aaaagtctca gcgaaaaccg gttcgttgca gggggtatat aacctggcgg
    52081 gattcattac cacagcgagc gggcaacgaa tggcgtttgt gcaatatctt tctggctatg
    52141 cagtagaacc ggcggatcag cgtaatcgcc gtattccgtt agtgcgtttt gaaagccgtt
    52201 tgtataaaga tatttatcag aacaattagt caataagaaa ccccggcgca tggccggggt
    52261 tttgctgatt aacgcttgta aatgaactca acgccttctt cgtcgtcttc gtcccagtcg
    52321 tcatcccagt cttcatcatc ctcttcagca atctcttcaa gctgctggcg atgataatca
    52381 tcccacatga attcgacttt ctctggctgt ttcgcttctt cagcctgcac gaccgggttt
    52441 tcaatgataa aggtcatcac atcccagcag agatctttca cgcccagtcc actcgccgca
    52501 gagatcagat aatatttatc ttcccagccc agcgcctcag cgatcgcttt cgctttctct
    52561 tcggcttcta ccttatccag cagatcgatc ttgttgaaga ctaaccaacg cggtttcgcc
    52621 gccagatcct ggctgtattt ttccagctcg ctgataataa tacgcgcgtt ttcaaccgga
    52681 tcggtgccgt caatcggatc gatatcgatg aggtgcaaca ggacgcggca acgttccagg
    52741 tgcttcagga agcgaatgcc cagacctgcg ccttccgcag cgccttcaat cagtcctgga
    52801 atatcggcaa caacgaagct cttttcgttg tccattcgta ccacacccag acttggcacc
    52861 agagtggtaa acggataatc cgccactttc ggtttagccg ccgataccgc acgaataaag
    52921 gtcgatttac ccgcgtttgg catccccaac ataccgacgt cagccagcag catcagctcc
    52981 agcagcagct cacgcttatc gcccggcgta ccgttagttt tctgccgtgg tgtacggtta
    53041 actgacgatt tgaaacgggt attgcccaga ccgtgccagc cgcccttagc aaccagcaga
    53101 cgctgaccgt gtttggtcat atcgcccatg gtttcaccag taccctggtc gattacacgc
    53161 gtacctaccg gcactttaat tgtgacgtct ttaccgcgct taccggtaca gtcgcggctt
    53221 gcgccattct gaccgcgctc tgcacggaaa gatttttcaa aacgataatc gataagcgtg
    53281 ttcaggttct cgtcggcttc catccatacg tcaccaccat caccgccgtc gccgccatcc
    53341 gggccgcctt tcggaatata cttttcgcgg cggaagctca cgcaaccatt accgccatca
    53401 cctgcaacga ccagaatcga tgcttcatca acaaacttca ttttattctc cgtaaatcat
    53461 tcgcctgcgc ggggttgcga taccaccgtt gtatgcttac gtaatccgcc ccaaatacga
    53521 tgaccaatgg cggaatacat cgcgcccgca accacgacaa acgcaccgag ataacctaaa
    53581 aggtttaaca tcggtctggc gaagaaatcg ggccaggcca gtgataaaag atctgaaaaa
    53641 aacagcgtaa acagtggggt gagcgtgatg atcgcgctca cctgcgctgc ctgccagcga
    53701 gccatcgctt ccgccagggc gccatatcct accaaggtat tcagtccgca aaaaattaaa
    53761 catgcgagct gccagtggct aagctgcgct atcactccag gctttgccag agggaagagc
    53821 gcaattgtac ataaagtgta cagtaaaaac aggatctgcg gtgaggccag ccgacgcaat
    53881 aaaacctttt gcgccacgcc ataactcacc caaaccgtcg ccgcaccgac cccaaagata
    53941 actccccagg tgtaatcggt gagctttgta aatatctcaa ccagactggt gttaaaaaac
    54001 atcaccagac cgctcaggag catcaatgcc cctacaacct gagtgctgcg cattttctct
    54061 ttcaggataa atacgctggc aaccatcatg ccaactggcg agagttgccc aatcacctgc
    54121 gaagcggtcg gactcaggta ttgcaaggat gagctgaaca ggatgaagtt accaaacagc
    54181 ccggcggtcg ccactgccaa caaaatcaac cagcgtggct tacgaaacac gcgtaatggc
    54241 ggcaacctct tcttcaccgc aagaatggca ccaaggccaa tactcgccat caagaaacgg
    54301 taaaacacga ttgtcggagg ttccatcacc tccagcacct gcttcattgc gattggcaac
    54361 gccccccagc aaattgctgt ggtgagcgcc aaaagaatgc caatgcctgc ctgctgcttc
    54421 ataccgtatt ccctgcaaag cgcatttgcg aattaacgtc gcaccatgcg catttttacc
    54481 ggttatcgaa tgtaaaaagc cccgcaacgt gttgcggggc tttcatccgt taccgggacg
    54541 cgaaaaactt attcagcttc gatgctgata aatttacggt ttttcgggcc tttaacttcg
    54601 aatttcactt taccgtctgc tttagcaaac agagtgtggt cacgaccgca acctacgtta
    54661 gcgccagcgt ggaatttggt accacgttga cgaacgatga tgctacccgc cagaacggat
    54721 tcgccaccga aacgcttaac gcccaggcgt ttagcttctg aatcgcgacc gttacgtgtg
    54781 gagccgccag cctttttatg tgccatttga aatctctcct caggtcttag gcgctgatgc
    54841 cagtaatttt cacatcagtg aaccactgac gatggccctg ctgcttacga tagtgtttac
    54901 gacgacgaaa cttaacgatt ttaactttct cgccacgacc gtgagcaaca acttcagctt
    54961 tgattacgcc gccatcaacg aaaggaacgc cgattttgac ttcttcaccg tttgcgatca
    55021 tcagcacttc agcgaactca acagtttcgc cagttgcgat gtccagcttt tccaggcgaa
    55081 cggtctgacc ttcgcttact cggtgttgtt taccaccact ttggaaaacc gcgtacatat
    55141 aaaactccgc ttccgcgcac accttttcaa tgattcagag tgcgctataa atattcacaa
    55201 tagggcgcga atattacgca aaacgcacgc ctttgacaag tgctacagtc aatacacgaa
    55261 gaaaaaaaac acaacttgta cggtaacgtt tatctgtgcc attttttcag tacaatcacc
    55321 ctatattcct aaccataaac cctaagttgc ctttgttcac agtaaggtaa tcggggcgaa
    55381 aagcccggct tttgcgatga atttagaaaa aatcaatgag ttaaccgcgc aagatatggc
    55441 gggtgttaat gcggcaatcc ttgagcagct taattccgac gtccaactga tcaatcagtt
    55501 aggctattac atcgtcagcg gcggcggtaa acgtattcgt ccgatgattg ctgtactggc
    55561 tgcacgagct gttggctatg agggaaatgc gcatgtcacc attgctgccc tgatcgagtt
    55621 tatccacacg gcgactctgc tgcacgacga cgttgtggat gaatcagata tgcgcagggg
    55681 taaagctacc gccaacgccg catttggcaa tgccgccagc gtgctggtag gcgattttat
    55741 ttatacccgc gcgttccaga tgatgaccag cctcggctcg ctcaaagtgc tggaagtcat
    55801 gtcagaagcc gtaaacgtca tcgcagaagg tgaagttctg caactgatga acgttaacga
    55861 tccggacatc actgaagaaa actacatgcg cgttatctat agcaaaaccg cgcgtctgtt
    55921 tgaggctgcc gcgcagtgtt ccgggattct ggctggctgt acgccggagg aggagaaagg
    55981 cctgcaggat tatgggcgct atctcggcac tgctttccag ttgatcgacg atttactcga
    56041 ttacaatgcc gatggcgaac agttaggtaa aaacgtcggc gacgatctga acgaaggtaa
    56101 accgacgctg ccgctgctgc atgcaatgca tcatggcaca ccagaacagg cacagatgat
    56161 tcgtaccgcc atcgaacagg gtaacggtcg ccatcttctg gaaccggttc tggaagcaat
    56221 gaacgcttgt ggatctcttg aatggacgcg tcagcgtgcc gaggaagaag cagacaaagc
    56281 catcgcagcg ttacaggtgc tcccggacac cccgtggcga gaagcactca tcggcctcgc
    56341 gcacatcgct gttcaacgcg atcgttaatc ccctccccgc atcccgcgca gcgcgcggga
    56401 tggttctagt aaattccata aaaacactta ttcagctcta taacacctga aaaatagggc
    56461 tgtacatctt tatatattct gaatattcac actctttaca ggaacttttt agaacaatac
    56521 gccatcagga gtatagtgat gcccgacaga agaaatgttc tgaatgaaag cgactaactt
    56581 aaggagtgag gaaagatgga aagtaatttc attgactggc atcccgctga catcattgcg
    56641 ggtttgcgca agaagggaac gtcaatggcg gcggaatctc gcagaaatgg tttgagttcc
    56701 tcaacgctgg cgaacgcatt atcgcgcccc tggccgaaag gagagatgat tattgcgaaa
    56761 gccctgggaa ctgacccctg gattatctgg ccatctcgct accatgatcc gcagacccat
    56821 gagtttatcg acagaacgca gttgatgcgt agctacacta aaccgaaaaa ataagtggtc
    56881 tggcggtagc cccgcgaacg gggctgccag ctctcagaca attactcgcc tttcacacgc
    56941 tcaatatttg cacctaaagc gcgcagtttg tcttcaatgc gttcgtagcc acgatcgatg
    57001 tgataaatac gatcaaccac cgtcgtccct tccgcaatac agccagccag caccaggctt
    57061 gctgatgcac gcagatcggt tgccataacc tgtgcgccag aaagtttttc aacaccgtga
    57121 caaataacgg tattgctttc gatttcggcg tgcgcgccca tacggctcag ctctggcaca
    57181 tgcataaagc ggttttcaaa gaccgtttcg gtgatgaagc cggtcccttc tgccaccagg
    57241 ttcaacagcg tgaactgggc ctgcatatcg gtcgggaatg ccggatgcgg cgcggtacgt
    57301 acgttaacag ccttcggacg tttgccatgc atatccaggc taatccagtc ttcgccgact
    57361 tcgatgtctg ctccagcgtc acgcagtttc gccagcacgg cgtccagagt atctggctgc
    57421 gcgttacggc agataatttt gccgcgagag atcgccgccg ccaccaggaa agtaccggtt
    57481 tcgatacgat ccggcagcac gcgatagaca ccgccgccta aacgttccac accttcgatg
    57541 acgatacgat cggtgccctg accgctaatt ttcgcaccca gcgtaatcag gaagttcgcg
    57601 gtatcgacga tttccggttc acgcgctgcg ttttcaataa tcgtggtgcc ttccgccagg
    57661 gttgcagcac acatgatggt caccgttgcg ccaacgctga ctttatccat cacgatatgt
    57721 gcgcctttca gacgaccatc gacggaagct ttaacgtaac cttcttccag tttgatggtc
    57781 gcgcctaatt gttcgagacc agaaatgtgt agatcaaccg gacgtgcacc aatcgtacaa
    57841 ccgccaggca gtgaaacttg cccctgacca aagcgcgcta ccagcggccc cagcgcccag
    57901 atagaagcac gcatggtttt aaccagatcg taaggtgcgc agaatacatt aacatcgcgg
    57961 gcatcaatat gcacagaacc attacgttct actttcgcac ccagctggct tagcagcttc
    58021 attgatgtat cgacgtcttt cagtttcggg acgttctgga tctctaccgg ttcttccgcc
    58081 agtagtgcgg caaaaaggat aggcagagca gcatttttag cgccggaaat tgtgacttcg
    58141 ccctggagct tcgttggccc ctgaacacga aatttatcca tttagtttgt tctcagttaa
    58201 caattcatat ccgctaccgg cgaatcgccc atagctcaaa agccgttcag tttgcgatcg
    58261 cgcgcccact ccgcaggggt atacgctttg atcgacacag catgaatgcg gttatccgca
    58321 atatattcca tcagcgggcc atagaccgtc tgctgttttt taacccgact catgccgtca
    58381 aacaactcac ccacggcaat aacctgaaag tggctgccat cgccggaaac gtggacttcc
    58441 tggagggaga gagcgttcat caacacgctc tgaatttcat tattttccat gggatcttca
    58501 atcatcagtt aataaaccag cgaaacatct tagagcaaag ttgcgctggc ataaataagc
    58561 aaaaagcctc gctgataaat cagacaaggc tcgacttgca ggcaggtttg ccggacaggc
    58621 ggttaacgcc atatccggcc tgaaaaaatt taacgaggca gaacatcagc aggcaaatta
    58681 tacaatttcg ccagggtata cactttgtcg tttaccccct gaagcgtcac gttgttgccc
    58741 tgctttttcg ccagatcgat aagatggagc agcagtgcca gtccacccgt atccacgcgg
    58801 gacacacggc taagatcgat gcaggtaatc cccttcaccg cttcctcacg catttcccaa
    58861 agcggtagca gaacgtcctg atccagctct ccggataacg ccagcgtgtc acccgtctgc
    58921 atccagctca gtgactcgct cattattttt tctcttccag agtgattttc tgttgagaaa
    58981 tcgatttcag ttgtgcagtc aggccgtcga tacctttggt acgcagcagc gttccccact
    59041 cgttttgttt ggtggtgatc atactgacgc cttcagcaat catgtcgtaa gcctgccagt
    59101 tgcccgtctg ggagttttta cgccactgga agtccagacg caccggcggg cggccattcg
    59161 ggtcaataat ggtaacgcga ataggcacaa tggttttatc gcccagcggc tgttctggcg
    59221 caatctgata ggtttgaccg tgatacatcg ccagcgcctg accgtaagcc tgcttcaggt
    59281 actcacggaa agcggcaaag taagcatcac gttgagcagg ggtcgcactc ttgtaatact
    59341 ggcccagcac cagcgcaccg gcgtatttca cctgtacgta tggcaacagt tcctgatcga
    59401 caatggtacg cagataatcc gggttggccc gaatttgcgg ttgctcattc ttcaggcgat
    59461 cgaacgtttt ctgcgccgcc tcgtccatca gcttatacgg attggtctgg tctgccgcgg
    59521 ttgccgcact cagcggtgca atcaccagca aagcgaccat cattaaacgt ttaaacatgc
    59581 gtcggttctc ctgaaattat ttcgttgtac ccacaggttc agtggtttca ttattacctg
    59641 gcgcagcagc tggcgcatcg ccactattct tattgtcatc gcctttacta ccgtaaagga
    59701 actgaccaat aagatcttcc agcaccatcg cagacttggt gtcctgaatt gtatcgccat
    59761 ccttcaggat agcagtcccc agttccgggt cttcaaaacc gacgtttaat gccagatatt
    59821 gttcccccag caggccggaa gtacgaatgc tcagcgaact ggtatcagga atgtggttat
    59881 aacgttgttc aatttccagc gttacgcgcg gcagataggt tttcgggtcc agcgtaatat
    59941 ccgccacccg gcccacaaca acaccaccaa tgctgaccgg agagcgcgct ttcaggccgc
    60001 caatgttatc gaacgtcgca taaagcgtgt aggtcggttc agtacgtatg gacgtcacgt
    60061 tcgccgcctt caggcaaaca aacagcgccg ccagtagtgc tgctaataaa aagataccca
    60121 cccaaatttc attttttttc gtttgcatga actcaattcc caaacatcaa tgcggtcagc
    60181 acaaaatcca gccccagaac agccagagac gagtggacaa cggtgcgagt ggttgcccgg
    60241 ctaatcccgg cagacgtcgg gatggcgtcg tagccgttaa acaacgaaat ccacgtcacc
    60301 gtgatggcga acaccacgct cttaatcaga cagttgacca gatccatacg ccagtcgacg
    60361 gcattttgca ttgccgacca gaagaacccg ctatcaatgc ctttccaact gacgccgacc
    60421 agagatccgc cccagatccc cacggcgacg aaaataaccg tcaacagtgg taatgaaata
    60481 accccagccc agaaacgggg agaaataacc cgacgcagcg gatccaccgc catcatctcc
    60541 atactggaga gttgctctgt agcgcgcatc aggccgattt ctgcggttag cgccgaacca
    60601 gcacgcccgg caaacaacaa cgcggcaacc accggcccca gttcacgcag tagcgataac
    60661 gccaccagca tacccagact ggtttccgca ctataagtgg tcagaaccag ataaccttgc
    60721 agccccaaca ccattccgat gaacacgcca gaaactacaa taatcagcat cgacaggacg
    60781 ccgacattat agagctggcg caccagcagc ggcgcatgtt tgcgaaattc cggtttgccg
    60841 accagcgcat tgaataacat taacccggcc cgcccgaacg ttctcagggt tttaatccct
    60901 ttatgtccga gcgacgccag cgcatttaac agcatgagtg gcttaactcc ctggtaaaag
    60961 atcagcgtga taatcgccgg caggatagcg gaacggaaca ggcccgtcag ctatcccgtc
    61021 cagaaactga cgtacgcgcg gatcaggatt cgcctgcaac gcctgggcac taccatgagc
    61081 gacaattttt ttgtccgcca ggatccaggc gtgatcggca atacttaaca cttccggcac
    61141 atcgtgagaa accaccacac aagtcacacc caacgcgctg ttcagctcag aaatcagctt
    61201 caccagtacg cccatggtga tgggatcttg cccaacaaag ggttcatcaa acatgatgag
    61261 atccggctcc agcgcaatcg cacgcgccag cgctgcacgc cgcgccatcc caccggaaag
    61321 ttcagaaggc attagtttag ccgctccacg cagccccacg gcctccagct tcatcatcac
    61381 cgtactatgc aacaatggcg cgggaagttg ggtatgttcg cgcagtggat aggcgacgtt
    61441 gtcaaatacg ttcatatcag tgaacaacgc cccggactga aataacatgc tcatccgttt
    61501 gcgcactgta tacaggcgcg aacgagacat cgccggaata ttctcaccat cgaaaagaat
    61561 ctcaccatga tctggtgcga tttgcccgcc aatcagacgg agtagcgtcg ttttaccgat
    61621 gcccgatggc cccatgatcg ccgtgatctt ccctcgcggc acggtcaggg aaatattatc
    61681 gaagatgcag cgattgccac gcgtaaaact gacatcgcgc atatcgacta aattcgccac
    61741 agactgctcc ataattcacc cttcgtcttg cgttgatttt ctaagcatgg cgctcaattt
    61801 aaccttgaac ccaacatatt tacagaatat tacccgccgt ggttagcgaa agctggcatt
    61861 tgttttactt tttagccgca taaagtcaaa attaagaatc cgttacggct ttctgaaaat
    61921 cttcagcgga ccggcgagta tacctgaaga aaggacgtta gatgctttta gctacggcac
    61981 tgttaattgt tggtttactt ttggtcgttt acagtgccga ccgcctggtt tttgccgcgt
    62041 ctattctttg ccgaaccttt ggcatcccgc cgctgatcat cggcatgacg gtggtcagta
    62101 ttggtacatc gttaccagaa atcatcgtct cgcttgccgc gtctttgcac gaacaacgcg
    62161 atttagccgt tggtacagcc ctcggctcaa acattatcaa tatattgctg atcctcggtc
    62221 tggctgcgct ggttcgtcct tttaccgtcc attctgatgt tctacgccgt gaattaccct
    62281 taatgttgtt ggtcagcgtg gtggccggtt ccgtactcta tgacggacaa cttagtcgca
    62341 gcgatggtat ctttctcctc tttctggctg tgctatggct gctgttcatt gttaaacttg
    62401 cacgtcaggc tgaacgtcag gggactgaca gcctgaccag agagcagctt gcagagttgc
    62461 cgcctgacgg tggattgccc gtcgcgtttt tatggctcgg cattgcgctt atcatcatgc
    62521 cagtggccac gcggatggtg gttgataacg ccacggtgct ggcgaattac tttgccatca
    62581 gcgagttgac gatgggtctg acggcaattg ctatcggaac cagcctgccg gaactggcaa
    62641 ccgcaatagc gggggttcgc aaaggtgaaa acgacattgc tgtcggaaat atcattggcg
    62701 caaacatttt taatattgtc atcgtgttgg gtttacccgc gctgataacg ccaggagaga
    62761 ttgatccact ggcgtacagt cgtgactaca gcgtgatgtt gctggtgagc attatttttg
    62821 cgttgctatg ctggcggcgc tccccgcaac cgggccgtgg tgtaggggta ttattaactg
    62881 gcggatttat cgtatggctg gcgatgttgt actggttatc gccaatactc gttgaataac
    62941 tggaaacgca ttatgtcgca cgtagagtta caaccgggtt ttgactttca gcaagcaggt
    63001 aaagaagtcc tggcgattga acgtgaatgc ctggcggagc ttgatcaata catcaatcag
    63061 aatttcacgc ttgcctgtga aaagatgttc tggtgtaaag ggaaagttgt cgtcatgggg
    63121 atggggaaat cggggcatat tgggcgcaaa atggcggcaa cgtttgccag caccggtaca
    63181 ccttcatttt tcgtccatcc tggtgaagcc gcgcatggtg atttaggcat ggtcacccca
    63241 caggatgtgg tgattgctat ctctaactca ggtgaatcca gcgaaatcac ggccttaatt
    63301 ccagtgctta agcgtcttca cgtaccgtta atctgcatca ccggtcgccc ggagagcagc
    63361 atggcgcgcg ccgcagatgt tcatctgtgt gttaaagtag cgaaagaagc ctgtccgtta
    63421 gggctggcac cgaccagcag caccaccgcc acgctggtta tgggcgatgc cctcgctgtc
    63481 gcgctgttaa aagcacgcgg ctttactgcc gaagattttg cgctctcaca tccaggcggc
    63541 gcactgggtc gtaaacttct gctgcgcgta aacgatatta tgcatacggg cgatgagatc
    63601 ccgcatgtta agaaaacggc cagtctgcgt gacgcattgc tggaagttac ccgcaaaaat
    63661 cttggtatga ctgtcatttg cgatgacaat atgatgattg aaggcatctt taccgacggt
    63721 gatttacgcc gtgtcttcga tatgggcgtg gatgttcgtc agttaagtat tgccgatgtt
    63781 atgacgccgg ggggaatacg cgtgcgccct ggcattctgg ccgttgaggc actgaactta
    63841 atgcagtccc gccatatcac ctccgtgatg gttgccgatg gcgaccattt actcggtgtg
    63901 ttacatatgc atgatttact gcgtgcaggc gtagtgtaaa gattcaagga taaacaacaa
    63961 tgagcaaagc aggtgcgtcg cttgcgacct gttacggccc tgtcagcgcc gacgttatag
    64021 caaaagcaga gaacattcgt ctgctgatcc tcgatgtcga tggcgtactg tcagatggcc
    64081 tgatttatat gggcaataat ggcgaagagc tgaaagcgtt caatgttcgt gacggttatg
    64141 gcattcgttg tgcgctcacc tctgatattg aagtcgctat cattaccggg cgaaaggcta
    64201 aactggtaga agatcgttgt gccacattgg ggatcactca cttgtatcag gggcagtcaa
    64261 acaaactgat cgcctttagc gatttgctgg aaaaactggc gattgccccg gaaaatgtgg
    64321 cttatgtcgg cgatgatctc atcgactggc cggtaatgga aaaagtgggt ttaagcgtcg
    64381 ccgtggccga tgcgcatcca ctgttgctcc cgcgcgccga ttacgtgacg cgcattgctg
    64441 gcggtcgtgg tgcagtgcgt gaagtttgcg acttattact cctggcgcag ggcaaactgg
    64501 atgaagccaa agggcaatcg atatgagtaa agccagacgt tgggttatca ttgtgctatc
    64561 actggcggtt ctggtgatga tcggcattaa tatggccgaa aaagacgata ccgcccaggt
    64621 ggtcgtcaac aacaatgatc ccacctataa aagcgagcat acggacacgc tcgtctataa
    64681 cccagaaggg gcactaagct atcgattgat tgctcaacac gttgaatatt attccgatca
    64741 ggccgtttcg tggtttacgc agccggtact taccacgttt gataaggata aaatcccgac
    64801 atggtccgta aaagcagata aagccaagct gaccaatgac cggatgctct atttatatgg
    64861 acacgttgaa gtcaacgcac ttgtgccgga ctctcaactt cgcagaatca cgacggataa
    64921 cgcgcagatc aatctggtga cgcaggatgt tacctctgaa gacctcgtca cgttatacgg
    64981 aacaacattt aactccagcg gtctgaaaat gcgcggcaac ttacgcagca agaacgccga
    65041 gctgattgaa aaggttagaa catcctatga aattcaaaac aaacaaactc agccttaatc
    65101 ttgtgcttgc cagctcactt ctggccgcca gcattccggc atttgccgta accggagaca
    65161 ctgatcagcc gatccacatt gaatcggacc agcaatctct tgatatgcaa ggcaacgtgg
    65221 ttacctttac cggtaatgtc atcgtcaccc agggcaccat caaaattaat gccgacaaag
    65281 tggtcgttac ccgtccgggc ggcgaacaag gtaaagaagt gattgacggc tacggtaaac
    65341 cggcaacgtt ctaccagatg caggacaatg gtaaacccgt tgaaggtcac gcttcccaga
    65401 tgcactacga actggcaaaa gattttgtcg ttctgacggg taatgcttat ctgcagcagg
    65461 tcgatagcaa cattaagggc gataagatca cttacctggt gaaagagcag aaaatgcagg
    65521 ctttcagcga caaaggtaag cgcgtaacca ccgttctggt gccgtcgcag ctgcaggaca
    65581 aaaacaacaa aggccagacc ccggcacaga agaagggtaa ttaattcgtt atggcaacat
    65641 taactgcaaa gaaccttgca aaagcctata aaggccgtcg cgtggtagaa gacgtcagcc
    65701 tgaccgtcaa ctccggggaa attgtcggtc tgctggggcc aaacggtgcc ggtaagacca
    65761 ccactttcta catggttgta ggcattgtgc cgcgcgatgc gggcaacatc attattgatg
    65821 atgacgatat cagtctgctg cctctgcatg cacgcgcgcg ccgcggtatc ggctatctgc
    65881 cgcaggaagc ctccattttc cgtcgcctca gcgtttacga taacctgatg gcggtactgc
    65941 aaattcgtga cgacttgtct gctgaacaac gtgaagaccg cgcgaacgag ctgatggaag
    66001 agtttcacat tgaacacttg cgtgacagca tggggcagtc actctccggg ggtgaacgtc
    66061 gccgtgtaga aattgcccgc gcactggctg cgaatccgaa atttattctg ctcgacgaac
    66121 cgtttgccgg ggttgacccg atctcggtta tcgacattaa acgcatcatt gagcacctgc
    66181 gcgacagcgg cctgggcgta ctgatcactg accacaacgt gcgtgaaaca ctggcggttt
    66241 gtgaacgcgc ttatatcgtc agtcaggggc atttgatcgc ccacggcacg cctacagaaa
    66301 tcttacaaga cgaacacgtt aagcgtgtat accttgggga agacttcaga ctctgatagg
    66361 gtagaagttt gcgacgtttt agcaggagag tacgattctg aacatgaagc aaggtttgca
    66421 actcaggctt agccaacaac tggcgatgac gccacagctc caacaggcaa ttcgtctgtt
    66481 gcagttgtcg acgctggaac ttcagcagga gctacagcag gcgctggaga gtaatccgct
    66541 gcttgagcaa atcgacactc atgaagaaat cgacacccgc gaaacgcaag acagtgaaac
    66601 gctggacacc gccgacgcgc tcgaacaaaa agagatgccg gaagagctgc cgctcgatgc
    66661 cagttgggac accatttaca ccgctggtac accatccggc accagcggtg actacattga
    66721 cgacgagctg ccggtctatc agggcgaaac gacgcagacc ttgcaggatt acctgatgtg
    66781 gcaggtcgag ttgacaccgt tttctgacac tgaccgcgct attgctacct ctatcgtcga
    66841 tgccgttgat gacaccggtt atctgactgt cccactggaa gatattctcg aaagtatggg
    66901 cgatgaagag attgacatcg acgaagttga agccgtcctt aagcggatcc aacggtttga
    66961 tccggtcggt gtggcagcaa aagatctgcg tgactgtctg ctgatccaac tctcccaatt
    67021 cgataaaacc acgccatggc tggaagaggc cagactgatc attagcgatc atctcgatct
    67081 gttagccaat cacgacttcc gcactttaat gcgcgtcacg cgtctgaaag aagatgtgct
    67141 gaaagaagcc gtcaatctga tccagtcgct cgatccgcgc cccgggcagt cgatccagac
    67201 gggcgaacct gagtatgtca ttccagatgt gctggtgcgt aagcataacg gtcactggac
    67261 ggtagaactc aacagtgaca gcattccgcg tctgcaaatc aaccagcact acgcctcgat
    67321 gtgcaataac gcgcgcaacg atggtgacag ccagtttatc cgcagcaatc tgcaggatgc
    67381 caaatggttg atcaagagtc tggaaagccg taacgatacg ctactgcgcg tgagtcgctg
    67441 tatcgttgaa cagcagcaag ccttctttga gcaaggcgaa gaatatatga aaccgatggt
    67501 actggccgat atcgcccagg ctgtcgaaat gcatgaatcg acgatatctc gcgtgaccac
    67561 gcaaaaatac ctgcatagtc cacgaggcat ttttgaactg aagtatttct tttccagtca
    67621 cgtcaatacc gagggcggcg gcgaagcttc ctccacggcg attcgtgcgc tggttaagaa
    67681 attaatcgcg gcggaaaacc cagcgaaacc gttgagcgac agcaagttaa cctctttgct
    67741 gtcggaacaa ggtatcatgg tggcacgccg cactgttgcg aagtaccgag agtctttatc
    67801 cattccgccg tcaaaccagc gtaaacagct cgtttgaccc aaccgataag gaagacacta
    67861 tgcagctcaa cattaccgga aataacgtcg agatcaccga ggcactgcgc gaatttgtta
    67921 cagccaaatt tgccaaactt gagcaatatt ttgaccgaat caaccaggtc tatgttgttc
    67981 tgaaagtgga gaaagtcacc cacacctcag atgcaacact gcatgtaaac ggtggtgaaa
    68041 ttcatgccag cgcggaaggt caggacatgt acgctgccat tgatggctta attgataagc
    68101 tggcacgcca gttgaccaaa cataaagata aactgaaaca acactaattg tccgggcaat
    68161 tagcatgtgc atggcggcct gttgtgcggc acaacgggcc atttgtacgg ttaatgctcc
    68221 gagcctgttc cactgtttga gtgggcaggt tcttaggtga aattatgaca aataatgata
    68281 caactctaca gcttagcagt gttcttaaca gggaatgtac gcgaagccgc gtccactgtc
    68341 agagcaaaaa acgcgccctg gaaatcatca gcgagctggc ggcgaaacaa cttagtctgc
    68401 cgcctcaggt ggtttttgaa gctatcctga cgcgggaaaa aatgggcagt accggtatcg
    68461 gcaatggtat tgccattccg catggcaaac tggaagaaga tactctgcgc gccgttggcg
    68521 ttttcgttca gctcgaaacg cctatagctt tcgatgccat cgacaaccaa ccggtggact
    68581 tactttttgc cctgctggtg ccggcagacc aaactaaaac gcacctgcat actctgtcgc
    68641 tggtggcgaa acgtctggcg gacaaaacca tctgccgccg tttacgcgca gcccagagcg
    68701 atgaagagct gtatcaaatc attacggata ccgaaggtac tccggatgaa gcgtagttat
    68761 tcggtaatgt ctcttttaga cgttgtgagg agaaacagta catggtactg atgatcgtca
    68821 gcggacgttc aggttcaggt aaatctgtcg ccctgcgtgc gctggaagat atgggttttt
    68881 actgcgtgga taaccttccc gtagtgttgt tacccgatct ggctcgaacc ctggccgatc
    68941 gcgagatttc tgccgccgtc agcattgatg ttcgtaatat gccggagtca ccagaaatat
    69001 tcgaacaggc gatgagtaac ctgcctgacg ctttctcacc gcaactactg ttcctggatg
    69061 ccgaccgtaa taccttaatt cgtcgttaca gtgacacgcg ccgactgcat ccgctttcca
    69121 gcaaaaacct gtcgctggaa agtgctatcg acaaagaaag cgatttgctg gagcctctgc
    69181 gttcgcgagc ggatctgatt gtcgatacct cagaaatgtc cgttcacgag ctggctgaaa
    69241 tgctgcgtac ccgtctgctg ggtaaacgtg aacgcgaact gaccatggtc tttgagtctt
    69301 tcggcttcaa acacggtatc cctatcgatg cagattacgt ctttgacgtg cgcttcttgc
    69361 cgaacccgca ctgggatccg aaactgcgtc caatgacagg tcttgataaa cctgtcgccg
    69421 cgttcctcga ccgccacaca gaagtacaca attttatcta ccagacgcga agctatcttg
    69481 agctatggtt acctatgctg gaaaccaaca accgtagcta cttgacggtc gccattggtt
    69541 gtaccggcgg gaagcaccgt tcggtgtata ttgcagagca actggcagac tacttccgct
    69601 cgcgcggtaa aaacgtccag tcacgccatc gtacgctgga aaaacgtaaa ccatgaccgt
    69661 caagcaaact gttgaaatca caaacaagct gggcatgcat gcccggcctg caatgaagct
    69721 gtttgaatta atgcaaggtt ttgacgcaga agtgctgtta cgtaatgacg aaggtaccga
    69781 ggccgaagcc aacagcgtta ttgcactgct gatgctggat tctgccaaag gacggcagat
    69841 tgaagttgaa gcgaccggtc cacaggaaga ggaagcactg gccgccgtta tcgccctctt
    69901 taattctggt tttgatgaag attaatcttc atcactttga cattcaaatc ccttcaagct
    69961 cccccgacaa taagaaaagc gcgtactgaa atcgttctca atcaacgtca tttgtacatt
    70021 tcgtgcgctt ttcactttca gaagaacatt aagaaaacct taagaagcat tgtttaggtt
    70081 tcgtttaagt taatcgacca tactggagat cgtcagaaaa tatttccagg agatggcatg
    70141 attcgcttat ctgaacaaag tcccctgggc accggaagac atcggaaatg ttatgcgcat
    70201 ccggaagatg cccaacgctg tatcaagatt gtctaccatc gtggcgatgg tggcgataaa
    70261 gagatccgcc gtgagttaaa gtactacgcg catcttggtc gccggttaaa agactggagt
    70321 ggaataccgc gctatcacgg taccgttgag acggattgcg gaaccggtta tgtctacgat
    70381 gtgattgcag attttgacgg caaaccctcc attacgctga ccgaatttgc tgcacaatgc
    70441 cgttacgaag aagacattgc acaactgcgc cagctactga aacagctgaa gcgttatttg
    70501 caggataacc gtatcgtgac gatgtcgctg aagccgcaga atatcctctg tcaccgcatc
    70561 agtgagtcag aggttatccc cgtcgtctgc gacaacattg gtgaaagcac attgattcca
    70621 ctggcgacct ggtcgaagtg gtgttgtctg cgtaagcaag agcggttatg gaaacgattt
    70681 atcgcccaac cggcgctgac tattgccctg cagaaagatc tgcaacctcg tgaaagcaaa
    70741 acgctgaccc tcacttcgcg cgaagcttaa tccagctggt gttgttgcat aaacgactca
    70801 ccgcctaact gatacatctg ccgtaaaatc cacgcctgac ggctacgcac gtagcccgat
    70861 ggcgcggaga ctttgaaacg aagtggatta ggtaatacag ctgccagtaa tgcagcttcc
    70921 gaccgggtaa gcttgctcgc gggtttgtgg aaataacgtt gtgccgcagc ttcgacgcca
    70981 aacacaccgt cgccaaattc ggcgatattc aggtaaaccg tcaggatacg ctttttgctc
    71041 cagaccgttt ctatccccag cgttaatccg gcttccagcc cttttcgaac ccagctacgc
    71101 ccatcccata aaaagagatt tttggccgtc tgctgagaaa tcgttgatgc accgcgaata
    71161 cggttttcat tgcgttcgtt gtgcgccagg gcttgctcaa tggaagcgac atcaaagccc
    71221 cagtgctcag gaaacttctg atcttctgcg gcaatcaccg ccagtcccat ccacggcgag
    71281 atttgatcca tgttgaccca gtcagaatgt gccacgtaac gaaaattgcc atgcagccag
    71341 gcgctgactt gccgctcgac cattaccgca gagaaaggaa caggcgcaac gctaaacaac
    71401 gcaatgcccc caccccagaa aacggcgagg acaaccatta accgcagaag aaaacggcga
    71461 atgaaactaa acaccgttaa gcggctttta ctcattccgc caggaccagc acgcgggaaa
    71521 ccagcttatc aatgccgctc gccgcttctg caatgttctg cgccagcata tatgccgggg
    71581 tggtgacaat cttattgtct tcatcaacca cgatatcatc gacaggacac ggcacatgct
    71641 ccgcgcccat ctcttccagc acttctgcgg tatcgatatc agtaccgatg gtcaaacgca
    71701 gcgggaaatc gaaaattttc ggcagcatcg ccggggcaat acacataaaa ccaagcggtt
    71761 ttccggcctg atgcatcgct tgtgccagcg cctttaattc acggtcaacg gtgcattcgc
    71821 taccaagact ggcaaaattg cttaaattct tcgccgcgcc aaaacccccc ggcacaatca
    71881 acgcatccag ttcagcggca tcggcctggg ccagaggacg gatttcaccg cgcgttattc
    71941 gtgccgcttc aatcagcaca ttgcgcgttt ccgtcatcgc ttcgcccgtt aaatggttga
    72001 taacatcaac ctgctgctta tccggtgcaa agcagaccgc ctgcgcaccg ctgcgtgaaa
    72061 tagctaacaa cgtcaacacc gcttcatgaa tttcagaacc gtcatagacg ccgcatccgc
    72121 tcagaattac gccaattttc ttcattgtga tcatcctttc gcaattgact gaaacacatt
    72181 aattttttta ataaaaatgc tacgcatcac acatttaact gattcatgta acaaatcatt
    72241 taagttttgc tatcttaact gcgtgcggcc tgaaaaacag tgctgtgccc ttgtaactca
    72301 tcataataat ttacggcgca gccaagattt ccctggtgtt ggcgcagtat tcgcgcaccc
    72361 cggtctagcc ggggtcattt tttagtggct tttgccaccc acgctttcag tacttctacg
    72421 tcgtgacgcc actcttcttt catctcttca atccattcac cgacgttatc ttcccaggcg
    72481 ggaaggtcag gagactgaat ttgctgaccc agctgttgca gatggcgtaa ccccactgac
    72541 cccgccgcac ctttaatttt atgtccttcc tcaacaatgc cttttttatc ctgcgccgtc
    72601 agattcgact ccagcacgct gacatagccc ggcatcatct tctcaaacac cgctaacccg
    72661 tcggtgatca gcttcggtcc tacaagttcg agatactgtt ccagcatggg aatatcgagc
    72721 aatgcttctg atttactgtt ctcttctgtc gtcaccgtac tctcctcatc atcctgggta
    72781 tcccagaatt tcttgatcat cgcggttagc gccggaacag aaagcggctt actcagcaca
    72841 tcatccattc cagcattgag gtactcttgc ttgtctttca gcacgttagc ggttaaggcc
    72901 accagcggcg gtaaatcctc gcgcggataa cgcttcgtca gttcacgaga gatatccagc
    72961 ccggtcatat ctggcaactg aatatccagc aataccaggt cgtattcgcc cggtttaaac
    73021 atctccagcg ccgccttgcc ggtcatggcg acatcaacgc tgttacctaa tttttccagc
    73081 acagaacgcg cgacaatcac gttcagttca atgtcttcca ccagcagcac attcagcgcc
    73141 ggtaaaggca tatcgtcttc atcaaacgca tcatcgacct cttcagccac cgacggtgcg
    73201 tggatcgtca acgtaaaggt tgaaccttta ccctgttcgc tggtaaccgt aatatcgccg
    73261 cccatatttt tcgccagacg acgagaaacg gccagaccaa taccggtgcc ggtggcaggt
    73321 ttaccgccat gactgtcttt cacctggtaa tacatggcaa agattttatc cagctcatcc
    73381 tgcggaatgc cgataccaga gtcttccact tcaaaatgca gcatatcgcc ttcatcgtag
    73441 cgcacgcgca cggtaacctg gccttgctgg gtgaatttga cggcgttact gatgagattc
    73501 cacaggatct gccgtaaacg cgtcccgtcg gtaatgacct gatgcggtaa tggcagcgta
    73561 ggctccagat taaagcgcaa tcctttttgt tgcgcctgca atgcggagag attttccaga
    73621 tcggcaagga agctggtgaa atcaaccggc tgattatcaa gctggacctt acgccgttcc
    73681 atcttatcca tgtcgataat atcgttgaag atattcccga gcgtgacggc cgaaacatgg
    73741 atggttttga gatatttttc ctgctcggcg gtgagttcgg tatccagcag aatgcggctc
    73801 aggccgacga taccattcag cggtgtacgc aattcgtgac tgatggtgga gataaacgtc
    73861 gttttgtcgc ggctggcccg ttcaagcgca tcctgatacc gcttacgctc ggtaatgtcg
    73921 cgaccaaaac ccatcaaacc gtgacgttta cccacgcggt cgtagtacgg cactttacgg
    73981 atttcaaaac aggctttgcg cccgtccggg taatccagcc actgttcata ggtcagtgac
    74041 acattatgac ggaacacttt ttcatcggtt tcaatgactt ttgcggcggc ttccggtgag
    74101 taaacatcag caggtttcag gtgaaccagt tgtttttcgc tttttccggt cagcagctcc
    74161 atcgcgcggt tacagccgga aaactcttta tcttcgttac gataaaaaac caggtcgggt
    74221 gaagcatcaa gaaaggaacg taagaatgag gattgctgtt cgagctgaat ttgtgtctct
    74281 tcgcgctctt taatttcaat tttcagttgg ccgaaggttt cctgcagttc tgcttccgct
    74341 ttttcacgaa cggcgatttc ctgatttagc tgggcaatat tatcttttaa ctgaacgttg
    74401 aggctcaaat cgcgctcgcg catctcctcc agtttttgca ccagccgtga cagacgttgc
    74461 cgtgactcct ccagttgctc gacgaccacc gatagaaagt agaccgccca cggcgtaatc
    74521 agcaaaccaa agaagataga acgaataaca tcaatgcttt cgacctgacc atgcagcacc
    74581 atggttaccg ccatttgtac cacaatggca agaacgacga gggccagcgc cagcaacatt
    74641 gagaagcgca ccagacctaa cttcatcatc aggtcaacat aatactgcgc cagcagacga
    74701 atttgcttca ttagggaatt ccttcacgac aacctgcccc aataataccc aattctgaca
    74761 gttacgttat aaattgtgtg agaaatgcgg aattcccctc aatggtctac tccgttggag
    74821 gtagccacgg gcgtcccagc gccgatccct gtacgccatg ttcattcaga tagcgatcca
    74881 gctcgaccat tcccgtccat cgattttcgc accacagcgg agcaagcaac gtcgggcgac
    74941 gggcgctggc ggaaatgcgg tggtagatca cttccggtgg cgtatggcga atcatttctc
    75001 cggcagtgag cgtgtaatcc tccagttcaa taccattcaa acgccccgct tcccaggctt
    75061 tcgccataat gctgcctttt actatatgca gcggatgcag ctttatgccg tccacgccgg
    75121 tttcaaccac tcgctccagc gtttgcaaac attcagcctg cccttcgcca ggcaggccga
    75181 caattaagtg agaacacacc ttcagcccgc gctcacgcgc cagctgcgtc gtgcgctgat
    75241 aacaggcaaa atcatgaccg cgattgatgc gatgtagcgt tttgtcgtgc gcggtttgta
    75301 gccccagctc cagccacact tcgtagccct ggtccttata ttcgcaaagc agatccaaca
    75361 ccgcatccgg cacgcagtcc gggcgggtac caacacacaa accgacaata ttggcctggc
    75421 tcaccgcctg ctgatacata gaacgcagca cctgaacttc cgcaaaggtg ctggtatacg
    75481 cctgaaagta ggccagatag cgtttagcgc ggttaactaa attcgcctgg tgcgccagtt
    75541 gttcggcaat ggaacgatgt tgctgcgctt catcggcaaa cgaggcaaca ttacagaatg
    75601 tgcagccacc acgcccgatg gtaccgtcac ggttagggca gctaaaaccg ccgtgcagcg
    75661 tcagcttatg caccttttgc ccataacgac gggtgagatc accaccaaac atattgacta
    75721 atttctgtaa ctgcataatc tgatagaccg cgccttgaaa agaggccaaa gcctgccatt
    75781 tttagctcaa ttcggcgatg acctggatca atcgtcccgg cctgctttta tcaactgcat
    75841 aatcaatcaa aattaccgaa atttcatgca taatcacata aatcactttt gcttatcttg
    75901 agtcagattt ttttatctcc ttatggattt taggcaaaaa cagtagcatg aaacgtcatt
    75961 accaattaag gcagtataaa atgctggttt tctcgtcagt tcaaggcagg ataagggtta
    76021 acacgccttt atgacagtca ggaattggct gtttctctaa cgactcccct tttgacctta
    76081 aatagaaaat ctattttaat ttcagttaat taataaagaa ttttgcgcta aagcacattt
    76141 ctgtaccaat aagcttgcca tttgacctgt atcagctttc ccgataagtt ggaaatccgc
    76201 tggaagcttt ctggatgagc agcctgctca tcatatttat gcagtaattg agatcccctc
    76261 ttcaccgtat taaccgatgc gaaaaggaca acaagggggc gaatgcgagg cgcgcgtatg
    76321 acacgcaacc cccgtcgcca cgctctttct gtgcccgtgc gcagcggttc ggaagtgggg
    76381 ttcccgcaga gcctggggga ggttcacgat atgttgtacg ataaatccct tgagagggat
    76441 aactgtggtt tcggcctgat cgcccacata gaaggcgaac ctagccacaa ggtagtgcgt
    76501 actgcaatac acgcactggc ccgcatgcag caccgtggcg cgattctcgc cgatggtaaa
    76561 accggtgacg gttgcggctt gctgttacaa aaaccggatc gcttttttcg catcgttgcg
    76621 caggagcgcg gctggcgttt agcaaaaaac tacgccgtcg ggatgctctt cctgaataaa
    76681 gatcctgaac tcgctgctgc cgcacgccgc atcgttgaag aagagctgca acgcgaaacg
    76741 ttgtcgattg tgggctggcg tgatgtcccc actaacgaag gcgtgctggg tgaaatcgcc
    76801 ctctcctctc tgccacgcat tgagcaaatt tttgtgaacg ccccggcagg ctggcgtccg
    76861 cgcgatatgg agcgccgtct gtttatcgcc cgccgccgca ttgaaaagcg tctcgaagcc
    76921 gacaaagact tctacgtctg tagcctgtcg aatctggtga acatctataa aggtctgtgt
    76981 atgccggcgg atctgccgcg cttctacctg gacctcgcgg acctgcgtct ggaatcggcc
    77041 atttgcctgt tccaccagcg cttctccact aacaccgtac cgcgctggcc gctggcgcag
    77101 ccgttccgct acctggcgca taacggtgaa atcaacacca tcaccggcaa ccgccaatgg
    77161 gcgcgtgcgc gtacctataa attccagacg ccgctgatcc ctgacctgca cgacgccgcg
    77221 ccgttcgtca acgaaaccgg ttctgactcc agttccatgg ataacatgct ggaactcctg
    77281 ctggcaggcg ggatggatat catccgtgcc atgcgtctat tagtgccacc tgcctggcag
    77341 aacaacccgg atatggaccc ggaactgcgc gccttctttg actttaactc catgcatatg
    77401 gagccgtggg atggcccggc aggcatcgtg atgtccgatg gtcgttttgc cgcctgtaac
    77461 ctcgaccgta acggtctgcg tccggcgcgc tacgtcatca ccaaagataa gctcatcacc
    77521 tgcgcctctg aagtcggtat ctgggattat cagcctgacg aagtggtcga aaaaggccgc
    77581 gtggggccag gcgaactgat ggttatcgac acccgcagtg ggcgcattct gcactcggca
    77641 gaaactgatg acgatctgaa aagccgccat ccatataaag agtggatgga gaaaaacgtc
    77701 cgccgactgg taccgtttga agatctgccc gatgaagaag tgggtagccg cgaactggac
    77761 gacgacacgc ttgccagcta ccagaaacag tttaactaca gcgcggaaga gctggactcc
    77821 gtaattcgcg tactgggcga aaacggtcag gaagcggtcg gttcgatggg cgacgacacc
    77881 ccattcgccg tgctctccag tcagccgcgc attatttacg attacttccg ccagcaattt
    77941 gcccaggtca ccaacccgcc aatcgaccca ctgcgtgaag cgcatgttat gtcgctcgcc
    78001 accagcatcg ggcgtgaaat gaacgtcttc tgtgaagcag agggccaggc gcaccgttta
    78061 agttttaaat cgccgattct gctctactcc gatttcaaac agctcacgac gatgaaagag
    78121 gagcactacc gcgcagatac actggatatc acctttgacg tcaccaaaac cacgctcgaa
    78181 gcgacggtca aagagctgtg cgacaaagcc gaaaaaatgg tacgtagcgg cactgtgctg
    78241 ctggtgctct ccgaccggaa tatcgctaaa gatcgcctgc cggttccggc tccgatggcg
    78301 gttggcgcga tccagacccg tctggtcgat caaagcttgc gttgcgatgc caacatcatc
    78361 gtcgaaaccg ccagcgcccg cgatccgcac catttcgccg tgttgctggg cttcggtgcg
    78421 acggctattt atccgtacct tgcctatgaa acgctgggcc gcctggtaga cacccacgcg
    78481 attgccaaag attatcgtac cgtgatgctc aactaccgta acggcatcaa caaaggcttg
    78541 tacaaaatca tgtccaaaat gggcatctcc accatcgcct cttaccgctg ctcgaaactg
    78601 tttgaagcgg tcggtctgca cgatgatgtc gtaggcctgt gcttccaggg cgctgttagc
    78661 cgcattggcg gggcgagctt tgaagacttc cagcaggatc tgctgaatct gtcgaaacgt
    78721 gcctggctgg cgcgcaagcc catcagccag ggcggcctgc tgaaatacgt gcacggcggc
    78781 gaataccacg cctacaaccc ggacgtggtg cgcacactgc agcaggcggt acaaagcggc
    78841 gagtatagcg actatcagga atacgcgaag ctggttaatg agcgtccggc aaccacgctg
    78901 cgcgatctgc tggcaattac gccgggtgaa aacgcagtca acattgccga tgttgaaccg
    78961 gcaagcgaac tgtttaaacg cttcgatacc gctgcgatgt ctatcggcgc gttaagcccg
    79021 gaagcccacg aggcgctggc ggaagcgatg aacagcatcg gcggtaactc gaactccggt
    79081 gaaggcggcg aagatccggc gcgctacggc actaacaaag tgtcgcgcat caagcaggtg
    79141 gcgtccggtc gcttcggtgt tactccggcg tacctggtca atgccgacgt cattcagatt
    79201 aaagtcgccc agggcgcgaa gccgggcgaa ggcggtcaat tgccgggtga taaagtcact
    79261 ccttacattg ccaaactgcg ctattcggtg cccggagtga cgctgatctc cccgccgccg
    79321 caccacgata tctactctat cgaggattta gcgcagctga tattcgacct caagcaggtc
    79381 aatccaaaag cgatgatctc cgtgaagctg gtttccgaac cgggcgtagg caccatcgcg
    79441 actggcgtgg caaaagctta tgccgattta atcaccatcg caggctatga cggcggcacc
    79501 ggcgcaagtc cgctttcatc ggtgaaatac gcaggctgtc cgtgggaact cggcctggtg
    79561 gaaacccaac aggcgctggt tgccaacggt ttacgtcata aaattcgttt gcaggttgat
    79621 ggcggcctga aaacgggcgt tgatatcatc aaagcggcga ttctcggcgc agaaagcttc
    79681 ggcttcggca ctggcccaat ggtcgcgctc ggttgtaaat atctgcgaat ttgccacctg
    79741 aacaactgcg ccacgggtgt agcaactcag gatgacaaac tgcgtaagaa tcactatcac
    79801 ggcctgccgt tcaaagtgac gaattacttt gagtttatcg cccgtgaaac ccgcgagctg
    79861 atggcacagc tgggcgtaac acgtctggtg gatctgattg gtcgcaccga cctgctgaaa
    79921 gagctggacg gtttcaccgc caaacagcag aaactggcgc tgtcgaagct gctggagacc
    79981 gccgagccac atgcaggtaa ggcactctac tgcaccgaaa acaacccgcc gtttgataac
    80041 ggcctgctga acgcgcagtt gctgcaacag gcaaaaccgt ttgttgatga acgccagagc
    80101 aaaaccttct ggttcgatat ccgcaacacc gaccgttctg tcggcgcatc gctttcaggc
    80161 tatatcgccc agacgcacgg cgatcagggt ctggcagccg atccgatcaa agcgtacttc
    80221 aacggcaccg caggccagag cttcggtgtg tggaacgcgg gcggcgtgga actgtacctg
    80281 accggtgatg ccaacgacta tgtcggtaaa ggcatggcgg gcggcttaat cgccattcgc
    80341 cctccggttg gctcagcctt ccgcagccat gaagcaagta ttatcggcaa cacctgcctg
    80401 tatggcgcga ccggtggtcg tctgtatgcc gcaggccgcg cgggtgaacg tttcggcgtg
    80461 cggaactccg gtgctatcac cgtggtagaa ggcattggcg acaatggctg tgaatatatg
    80521 acgggcggta ttgtctgtat tctgggtaaa accggcgtga atttcggcgc gggtatgacc
    80581 ggaggcttcg cttacgttct cgatgaaagc ggcgatttcc gcaaacgcgt taacccggaa
    80641 ctggtcgagg tcttaagcgt tgacgatctg gcgatccatg aagagcatct gcgcggtctt
    80701 atcaccgagc atgtgcagca taccggttcc cagcgcggtg aagagattct ggcgaactgg
    80761 tcaaccttcg ccacgaaatt cgcgctggtt aaaccgaagt ccagtgatgt aaaagcactg
    80821 ctgggtcacc gtagtcgtag cgcagcagag ttgcgcgtgc aggcgcagta aggggtagca
    80881 acaatgagtc agaatgttta tcaatttatc gacctgcagc gcgttgatcc gccaaagaaa
    80941 ccgctgaaga tccgcaaaat tgagtttgtt gaaatttacg agccgttttc cgaaggccag
    81001 gccaaagcgc aggctgaccg ctgcctgtcg tgcggcaacc catactgcga gtggaaatgc
    81061 ccggtacaca actacatccc gaactggctg aagctcgcca acgaggggcg tatttttgaa
    81121 gcggcagaac tgtcgcatca gaccaatacc ctgccggaag tgtgcggacg tgtctgcccg
    81181 caagaccgtc tgtgcgaagg ttcctgcacc ctgaacgatg agttcggcgc agtgaccatc
    81241 ggcaacattg agcgctatat caacgataaa gcgttcgaga tgggctggcg tccggatatg
    81301 tccggcgtga aacaaaccgg taaaaaagtg gcgattatcg gcgcaggccc ggcgggtctg
    81361 gcgtgcgcgg atgtcctgac gcgcaacggc gtaaaagcgg tcgtcttcga ccgtcacccg
    81421 gaaattggcg gattgctgac cttcggtatt ccggctttca agctggaaaa agaggtaatg
    81481 acgcgccgcc gtgaaatctt caccggcatg ggtattgaat tcaaactcaa taccgaagtg
    81541 ggccgcgacg tacagctgga cgatctgctg agtgattacg atgccgtgtt ccttggcgtc
    81601 gggacttatc agtcaatgcg cggcgggctg gaaaacgaag acgccgatgg cgtgtacgca
    81661 gcgctgccgt tccttatcgc caacaccaaa cagttaatgg gctttggcga aacccgcgaa
    81721 gaaccgttcg tcagcatgga aggcaaacgc gtggtggtac ttggcggtgg cgacactgcg
    81781 atggactgcg tgcgtacgtc tgtacgccag ggagcgaagc acgttacctg tgcctatcgt
    81841 cgtgatgaag agaacatgcc aggttcccgc cgcgaagtga aaaacgcgcg agaagaaggt
    81901 gtagagttca aattcaatgt ccagccactg gggattgaag tgaacggtaa cggcaaagtc
    81961 agcggcgtaa aaatggtacg taccgaaatg ggcgaaccgg atgccaaagg ccgtcgccgc
    82021 gcggagatcg tggcaggttc cgaacatatc gttccggcag atgcggtgat catggcgttt
    82081 ggtttccgcc cacacagcat ggaatggctg gcgaaacaca gcgtcgagct ggattcgcaa
    82141 ggtcgcatca tcgccccaga aggcaacgac aatgccttcc agaccagtaa cccgaaaatc
    82201 tttgctggcg gcgatatcgt ccgtggttcc gatctggtcg ttaccgccat cgccgaaggt
    82261 cgcaaagcgg cagacggcat tatgaactgg ctggaagttt aagctctgtc ccagcgccgc
    82321 ctcctcagaa aagagtcggc gctgaaattc tcctttcgtt ctggaaagcg cctcgcataa
    82381 tctgaagaaa accaatgtaa tacgcacatc tggattgtta ccggcagatg cccggcgtat
    82441 agctgattcc attattcaac actgcgccat attaagggac agataatgga atcgctctca
    82501 gaaggaacca cagcaggcta ccagcaaatc cacgacggca ttattcatct ggtcgatagc
    82561 gcccgaacgg aaacggtacg tagcgttaac gcgttaatga ccgcgacgta ctgggaaatt
    82621 ggccgacgaa ttgtcgaatt tgaacaaggt ggcgaggcca gggctgcgta tggtgcgcag
    82681 ctaatcaagc gactatcaaa ggatttaagt ctaaggtata agcgtggatt ttccaccagg
    82741 aatttatggc agtttaagaa tttttatatt tgttttcaaa gaattgaaat tgtgcagaca
    82801 ctgtctgcac aattcacccc cacaattctc cagactgtgt ctggagaatc ttgccattta
    82861 gcgcaactcg ccaaatcctt ccccctgccc tggtccactt acgtccgttt gctttctgtt
    82921 aaaaaagctg atgctcgcag cttttatgag aaagagacac tccgctgtgg ctggtctgct
    82981 cgtcagctcg aacgacaaat tgcgacccag ttttatgagc ggacactact gtcacatgac
    83041 aaatcagcca tgctgcaaca acacgctcct accgagacgc atattcttcc gcagcaggcg
    83101 atacgcgatc cctttgtgct cgaatttctg gaattgaaag atgaatactc agaatccgat
    83161 tttgaggagg cgctgatcaa ccacctgatg gatttcatgc tggaacttgg ggatgatttt
    83221 gcctttgttg gtcgacagcg aaggttacgc attgatgaca actggtttcg tgtcgatctg
    83281 ctgtttttcc accgccgttt acgctgcctg ctaatcgtcg atctaaaagt gggcaaattc
    83341 agctatagcg atgccggaca gatgaatatg tatctcaact acgccaaaga gcactggaca
    83401 ctaccggatg aaaatccgcc catcggtctg gttctctgtg cagagaaagg agccggcgaa
    83461 gcgtattatg cactggctgg tttgcctaac accgttctgg cgagcgaata taagatgcaa
    83521 ctacctgatg aaaaacgact cgcggatgaa ctcgttcgaa cacaggcggt gctagaggaa
    83581 ggctatagac gccgttaatt tcagcggttt cgaattctgc agacagtgtc tgcagaatct
    83641 ttaaaacgaa ctaagtttta agccattaaa tcagccttaa ccttcaccac aaccttttta
    83701 atctcatcag gttcgccgac aacgcatcct ggtttatgcg gttctcccgg cataaacacg
    83761 gcgaacattc ccggttttaa gatgatggct tgctcgttct caatggcgct gcaaagctgg
    83821 taatcatcct catggtggaa ctcttcacac tgacgcgcag tgcctgccgt gccaaacaga
    83881 atccgttcct caccgtttaa caacagctgg atatcaatgt attgctcgtg caattccgct
    83941 tttttctcga cgggcgattg agtgttaaac gtcatgacat tcataaagat attgtcgccc
    84001 tgtaattcgt aacgacccgg tgctttttct tgcggcctgg cggctaaggc tagtgttaac
    84061 gcgtcctgta acgcaggatg caatccggca gacggtaatg actgtacttc acccatcatc
    84121 ataatttttc tccctgggcc aacagcgcag ccccaagtaa acctgcatcg tggcggtagt
    84181 gcgccgccag taaatcaaca tgaaatgccg ctggctcctg cgccagatac gtttccacca
    84241 gcgccagata cccctctgcc agaccaacgc tgccaccgac caccacgcac tggcaatcag
    84301 ttgtggcttt aatatcagcg atcagccttg caagcgtacg tgcggaacgg tgaatcagtt
    84361 gctgcgcctg ctcatcgccc tgcccggcat gcgtgaaaat agttttcgca ttcgcaccag
    84421 ccaactcccc ttgcgccgcc gccgcaatgc cgcgaccaga agcaatcgcc tccacgcaac
    84481 ctgtgcgtcc acagccgcag actggaccat gcggatcggc aagcgtatgc ccgatgtgcc
    84541 ccgccagacc gccagggccg gtaagcagtt tgccgccgct cactacaccg ccgccgacgc
    84601 cagtggaaac ggtgataaac accatctcgg ttacatcgcc ttccagcgcc tgatactctg
    84661 cccatgctgc ggcctgcgcg tcgttaatgg caatggtcgg caaattggta agttgttcca
    84721 gcgttttgac taacggaaag tgtagtaatc caccgagatt atgcggatta agcgccagca
    84781 aactgccgtc gcgaataatt ccggttgaag cgatggcaac ccgctgcgca tgagcttgca
    84841 acggagagac taatgcggat aaggcatcac gcaaggcttc tggtgtctgg ctggctggcg
    84901 taggaagttc acgacgatcg cggatctgcc catcagcgcc aatcagcgcg gcggcaagtt
    84961 tagtaccgcc gatatcaatc gccagtgtgg tcatagcacc gcctttttca tcgctgtgtt
    85021 gtaccactga caaatgtgtt caagacgcgt aatggcagaa ccgaccgtca ccgcccacgc
    85081 gccgtggcgc atcgcatccg ccgcctgagc aggcgtgttg taacgccctt cggcaatcac
    85141 ccgacatccg gcgtcgctca acgttttcac cagcgccaga tccggctctt ctggcgtttc
    85201 aggcgtggta tagccagaaa gcgtagtgcc aataatttcg gctcccagct tttggcatgc
    85261 caggccgtct tccggcgttg agcagtcggt catcgccagt aaaccgtgat ggtgaatacg
    85321 tgccagcaac gtttcaacag gcaccggacg cgggcggtcg gtgccgtcaa tggcgataat
    85381 gtccgcgccc gcctgcgcca gcgcatcaac atcttcaata taggccgtga tgcgtaccgg
    85441 agaatcctcc agatcgcgtt tcacaatccc gataattggc acgctcacta ccgcacgcgt
    85501 ggcttgcaga tttgccacac cttcaatgcg aatggcaacc gcgcccgcct gctctgccgc
    85561 taatgccatg gcggcgacga tttcgggttt atcgagcggg ctgtccggaa ccggctggca
    85621 ggagacaatc aggccaccgt tagcagcgat tttttgatcc agttgtgcaa gtaacgacat
    85681 acatcttccc ttagcgaaag gcccggtaca gagaccgggc aacaggatta acttttggtt
    85741 ttgactaaat cgtttttggc gcggccaaat ggcacggcac cgctaaatgg tttgccgtcg
    85801 atagcgtcat gagtacgcaa tgcttccggg cgcagccaac gctgaacgcg ggaaggcata
    85861 tccagaccaa tcagcaggat caccacaaat gtcagactga acgagagcga tgccagcgca
    85921 gtacccagat ccagacgttg agcgatcaac gcgccgatga ttggggccag tgcaccgccc
    85981 aatgcgccga cgttgtaggt aaagcccagg cccgctgcac gctggtcggt atcgaaataa
    86041 ccgccaatca gtttcggtaa gatcccggcg atcccttgtc caagcatttg ctggaagaac
    86101 agtaacagac cgagcaccca gacgtttgcg ccgccaatcg caaataccgg aataatcagc
    86161 agctgcgagg ccagcaggct acaaacgtac gctttgcggg ttcccagcca gtcaccgagg
    86221 aagccaccta cgcagcatcc caccgccgcg ccaaagccac taaagaacag cacattggcg
    86281 acagtatgcg ggttataagc cagatcggtt ttcagatacg ttggcagcag cgcctgaatc
    86341 ggccatgagt agaggaaagc aaacaacacg accaccatca gcattacgcc cgttggccag
    86401 cgtttgcccg tactttgcac cataaagctg ataaagattg cggcgcataa cagcccaaga
    86461 acagcgacga tcgcggcatt ttgcaggtta ccggcgaagc aaaaccacag cgcagtagcc
    86521 gccgccagtg tcattacgat attggcaatg cgatgttcac cacggtagag aatatctacc
    86581 atcgtgcgta ctggtgcttt accagcatgt ttctctttcc agtcttccgc ttccgggatg
    86641 tttttacgca gccagagagc aaagatgatt ggcaaaatgc cgataaagaa cagcgcacgc
    86701 cagccccaga ccggaaccac caggctatag acctgagcgg caacgacggc ccccacagag
    86761 aagccggaaa tcagaaaacc actggcttta ttacgcagat gttttggcca gctttcaatg
    86821 acataggtgg cgctggaacc gtattcaccc gccatcccca tgccgatgac cagacgagcg
    86881 ataaacatgg tgatgtagcc tggcgcaaag ccgcaggcca gcgtcccggc cgagaagaga
    86941 acgatgctgg tgaccattgc cagacgacgc ccgtagcggt cacccatagc gccgagcatc
    87001 aggccgccga accagcgaga gataaaggct gcggagatca gacttgccgc ctgcaccgtc
    87061 gtcagcccga attcaccttg tacttcggtg agtaccaggg cgattaaaac gaaatcaaaa
    87121 ccgtcaagca gatatcccaa ccaggcagcg gaaaatgccc gccattgtgc acgattgaga
    87181 tggcgatacc acgggatatt ctgggttgaa gtactcattg tgagtctccc gcggtgggcg
    87241 atgcccacac gctttggtat gaaaattgta gggtacagat gcgtttattt cccctcaccc
    87301 ggtaggggcg agcgagggga aacaactcac ccgcgctctt gcatcaactg ctgggccaac
    87361 gccttcagtt ctggcagata tttttcatct accggtccaa acggtttgcg gcacagcggc
    87421 acagaaacga catccatata atgcaggacc gttttcaggc cgcggaatac gcccgttttg
    87481 atcagtaaat caatgacttt attacattcg gtttgcagct tctgcgcggt ctggatatcg
    87541 ccttctttca gcgccttcac aatcccctga taacgccagc ccatgatgtt gtaggtacta
    87601 ccgataccac catcagcgcc cgccagaaga ccagaggcga agatttcgtc gtaaccgtta
    87661 tagagcacca gatcaggatg ttcacgacgg atctgttcca tctgatagag atcgccagaa
    87721 gtctgtttca gcgcaccgac gccaggcaac gtaacaagtg tgttgatttg atccagggtc
    87781 agttttaccc cactcagggc tggaatgttg tacaccacca tcggcaaccc atccgctgaa
    87841 tcaataattg cccgatagtg atcgcagtgt tcttcaaagc tgaaaggata gtagaacggc
    87901 gtgacggcgg agacggcatc gaagccataa cgtttagccg atgccgcaag ttgttggctt
    87961 tcggcggtgc tgacgcaacc gacgtgggcg atgagtttaa tcttaccttt cgcctcttcg
    88021 gcgacgattt ccagtacctg ttcacgctcg gaaaggcttt gtacaaaggc ttcgccggtc
    88081 gaaccaccca cgtataaacc gtcaatgccc tgctgaatat tgaactgaac caggcgacgc
    88141 agactcgctt tatccagtgc ttgttgttga tcaaaaggag tcaggagtgc agccattacg
    88201 ccacgtaaat tcgttgccat aaatacctct gaagtgatgc ttgtctgata aacgatatac
    88261 ctttatacct gttataccag atcaattaag caacacccca tacagaaagc ttataatgcg
    88321 atctgcttca ctaaagtggc attatttctt tttgttggtg gtctgaccga aggcgtgcca
    88381 ggtagcagag acgctgttga gatgcgattg caacgcacga tcggcttcgt caggatcatg
    88441 acgccggatc gcgtcaacga tcgcaatatg ctgttgatag ctaacgttgt tatgctcgtg
    88501 cagagcctga tcagccacgg ttggacgtgc ggcgatcagc cagtcgagca gggcaacgtg
    88561 gatcgccatg aagattgggt taccggggat ctccgccaga acgcggtgga aatcaacgtc
    88621 tgagcgaatg aatgccgcgt tgttatccag cgactgactg ttgatttcca gtgcttttgc
    88681 cagcaaatcg atttgctcat cggtggcgtg ttcagccgca tagcgcacca ggctggattc
    88741 aaagaacaga cgtaattgtt cgaaatgggc aatcccgcca ggatgagaaa ggaaatcttt
    88801 cgccatgccg gaaagctcgc cgatgatggt gtccgcagaa ggacgtgaga cgcgggcacg
    88861 ttcgccgttg tttatctgca ccagaccttt gcgttttaac gctgccagcg cttcgcgcac
    88921 cgaaggacgc ccgacgttaa agaacgccat cagttcgcgt tcagacggta attgttcacc
    88981 ttcgccaaat tcacgacggc ggatcatctg ttccagctct tcttccacca tttcggagag
    89041 ttttttacgc gccagcgggc ggctacgcaa gttgcgacca attgcaggtg aagaatcttc
    89101 ggtttgcgaa tcaaatgcgt tcataaggcc cattctgtaa ggtcagtgtg attaacatca
    89161 tcagtgacat cctatcacag gattgaaagt aggggaaaat ggcagggttt tctctttgtg
    89221 cctcatcatt accataatta acggaataat taactattgc gaaaaattaa tgtaacgcag
    89281 ataaaaacat cccgattgaa ttattcataa gactattaac aagtattatg aatattatga
    89341 atgtgttctt acaaaataat cataagcgca tattttttaa tgaaaaatca cctcatctac
    89401 aattaaaaac acgacatccg caccataaat agccttgcaa aaaatataac atcattgttt
    89461 tcaatctgcc gtttatggga ttgaccgttt tcttttgaca cggagttcaa caatgttcgg
    89521 cataattata tctgtcatcg tattaattac gatgggctat ttgatcctga aaaactacaa
    89581 acctcaggtg gtgctggctg ccgcaggtat cttcctgatg atgtgcggtg tctggttagg
    89641 gttcggtggt gtgctcgatc ccgccaaaag cagcggctac ttgatcgtcg atatttataa
    89701 tgaaatcctg cgcatgctgt ccaaccgcat tgccggattg gggctgtcga ttatggcagt
    89761 gggcggttat gcccgctata tggagcgcat aggggcaagt cgcgcgatgg tgagcttgtt
    89821 aagccgcccg ttaaaactca ttcgctcgcc gtatattatt ctgtcggcaa cttacgtcat
    89881 cggccaaatc atggcgcagt ttattaccag cgcctccggt ctgggtatgt tgctgatggt
    89941 caccttattt ccgacgctgg tgagtctggg agtaagtcgc ctctctgcgg tggcggttat
    90001 cgcaaccacg atgtccattg agtgggggat tctggaaacg aactccattt ttgctgccca
    90061 ggtcgcagga atgaaaattg ccacttactt cttccactac cagcttccgg tcgcctcttg
    90121 cgtcattatc tcggtggcga tctcccactt tttcgtgcaa cgcgctttcg acaaaaaaga
    90181 taaaaatatc aatcacgaac aggcagagca aaaagctctc gataatgtcc cgccgctcta
    90241 ttacgccatt ttaccggtga tgccgttaat cctgatgctc ggctcgctgt tccttgccca
    90301 cgtcgggctg atgcagtcag aactgcatct ggtggtggtg atgttactga gtttgacggt
    90361 gacgatgttt gttgagttct tccgcaagca taacttgcgc gaaacaatgg atgatgtgca
    90421 ggcgtttttt gacggcatgg gtacgcagtt tgccaacgtg gtaacgctgg tggtcgcggg
    90481 tgaaatattt gcgaaaggct taacgacgat tggcactgtc gatgcggtta tcaggggtgc
    90541 ggagcattct ggtctgggcg gtattggcgt gatgattatt atggcgctgg tcattgccat
    90601 ttgtgccatt gtgatgggct caggcaatgc gccgtttatg tcatttgcca gtcttattcc
    90661 gaatatcgca gccggactac atgtgccagc ggttgtaatg attatgccga tgcattttgc
    90721 cacgacgcta gcgcgcgctg tttcgccgat tactgcggtg gtggtcgtta cgtcaggaat
    90781 tgcaggcgtt tcgccttttg cggtagtgaa gcggacagcg atccccatgg cagtcggttt
    90841 cgtggtgaat atgattgcca caatcacgct attttattaa gtcattaaaa agacaaaaca
    90901 ggccgccagg gcctgtttta gtattacttc acaacgcgta atgctggtcg accaccacgt
    90961 ggtggctgcg gtggttcatc gtcaggatga gtgtcatcat cgtgatctgg cttgtcgcca
    91021 tcaataaccg acataacggt ttcgttgtct gccgatgcct cttcatcatt catgatgctg
    91081 gcatcttcat cgtaggcagc ttcaggttca aacatcgtgc ctgcgccatt ttcacgggcg
    91141 tagatagcca gcacggcagc cagcggcaca gaaacctgac gcggaatgcc accaaagcgc
    91201 gcattaaagc gcacctcatc attcgccagt tccagattgc cgacagctcg cggagcaatg
    91261 ttgagtacga tttgcccgtc acgcgcatat tccataggaa cctgcacgcc agggagcgtc
    91321 acatccacca ccaggtgcgg cgtgagctgg ttatccagca accactcata gaatgcacgc
    91381 agcagatagg gacgacgtgg tgttagctgt gacaaatcca tacagattaa ctccggccca
    91441 gacgcatttc acgttcagct tcagttaaag aagcaaggaa agagtcacgc tcaaagacgc
    91501 gggtcatata gcctttcagc tctttcgcac ccgggccgct gaactcgatg cccagttgcg
    91561 gcaaacgcca cagcagcgga gcaaggtagc aatcgaccag gctgaactca tcgctcagga
    91621 agtacggctt ctgaccgaag accggcgcaa tcgccagcag ttcttcgcgc agttgcttac
    91681 gtgcggcatc tgcttcagaa gctgaaccgt tgatgatggt gttcatcagc gtgtaccagt
    91741 ctttttcgat gcgatgcatg tacagacggc tttcaccgcg agctaccggg taaacaggca
    91801 tcagtggcgg atgcgggaaa cgctcatcca gatattccat aatgatgcga gattcccaca
    91861 gggtcagctc acgatccacc agggtcggaa cgctctgatt cgggttgagg tcaatcagat
    91921 cctgaggcgg attgtccttt tccacgtgtt cgatctcgaa acttacacct ttctcagcca
    91981 gcacaatgcg gacctgatgg ctatagatgt cagtaggacc ggaaaacagc gtcattaccg
    92041 aacgtttgtt ggcagcgaca gccatgaaaa cctccaggta tagtcagaat ttttactgct
    92101 accagccacc aggtggccag tcagaagttg tgttacccag taaggaacga ctctctttgt
    92161 tcgaaaatca aacaaaaaat gagcaatacc cgacatttgg gcaaaaaatt ggatgatagt
    92221 ttaccagatt ttgcgacctt tgtggtgagt cgatgccgga aatggggaaa aagagatgcg
    92281 ctttagtctg aaatagttga cttagtccct tattggcgat gtggtttttg ttttgcctgt
    92341 ctgtcagacg gcagcaaaaa gcaactttcc attttttacg ctgattcaga atttagccat
    92401 aaaaaaaccc gccgaagcgg gttttttcgc aaattgtttt ctgccggagc agaagccaat
    92461 taacgtttgg agaactgcgg acgacgacgt gctttacgca gaccgacttt cttacgttca
    92521 acctgacgag cgtcacgagt aacgaagcca gctttacgca gttcagaacg cagggactcg
    92581 tcgtattcca tcagagcgcg ggtgataccg tgacggatcg caccagcctg accagagata
    92641 ccaccacctt taacggtgat gtacaggtcc agtttctcaa ccatgtcgac cagttccagc
    92701 ggctgacgaa ctaccatgcg ggcagtttca cgaccgaagt actgttccag agaacgttgg
    92761 ttgattacga ttttaccgtt gcccggtttg atgaaaacgc gagctgcgga acttttgcgg
    92821 cgaccagtgc cgtagtattg attttcagcc attgcctata atcccgatta gatgtcaaga
    92881 acttgcggtt gctgtgccgc gtggttgtgc tcgttacccg cgtaaacttt cagtttacgg
    92941 aacatagcac gacccagcgg gccttttggc aacatgcctt taaccgcgat ttcaatcaca
    93001 cgctcaggac ggcgagcaat catctcttca aaggtcgctt gtttgatacc accgatgtgg
    93061 ccggtgtggt gatagtacac tttgtcagta cgcttgttgc cggttacagc aactttgtca
    93121 gcgttcagaa cgatgatgta atcaccggta tctacgtgcg gagtgtattc cgctttgtgc
    93181 ttaccgcgca ggcgacgagc cagttcagta gccagacggc ccagagtttt accggtcgcg
    93241 tcaacaacat accagtcgcg ttttacggtt tctggtttag ctgtaaaagt tttcattaaa
    93301 agcttaccca ataaatagtt acacgttggt gaacacccaa acgtcttcaa ttgttgaggt
    93361 tcacacgaca aagtccggca aacctacccc ttcgaatagc ctatgccagc acacaaaaag
    93421 ttttgggaaa aaaactttct tgtaacgtgg ggtcgcagga ttatagagaa gtcggggtca
    93481 aagatcgacc cctttttgtg atttgtggca ggttttaacc cgccaaatgc tcgcgcttca
    93541 gatactcttc gctttgcatc tcttgcagac gtgacaggca acgctggaac tcaaacttca
    93601 gccgatcgcc ctgataaatt tcatacagcg gcacttctgc actcaccact aatttgacat
    93661 ggcgctcgta aaactcatcc accagcgcaa taaagcgccg cgcttcgctc tccatcaacc
    93721 gcgtcataag tggtacatca aacaacatga ctgtgtgaaa gaggcgtgag agcgcaatat
    93781 agtcatgctg actgcgggcg tcgacgcaca gcgtagtaaa agagaccgcc agcgtctggt
    93841 tctcgacgcc cattgtcgcc aatggccgat ggttgatttc taacgtcggt gaattttctc
    93901 gtttcgcccc cgccagcgcc aaccatagtt tatccatttg cgcccgggtt tcatcgttga
    93961 ggggcgaaag ccacagatgc gcctgagtga gtgtacgcag tcgataatca acaccagcgt
    94021 ccacgttcat tacatcacaa tactgtttaa tggcatcgat tgcaggcaaa aaacgcgcac
    94081 gttgtaggcc atttcgataa agttcgtccg gcggaatatt tgacgtcgct accagggtaa
    94141 taccgcgggc gaacagggct ttcatcagac cgccaagtag catggcatcg gtaatatcag
    94201 aaacaaaaaa ttcgtcaaaa cagagcacgt cagtttctgc tttaaagcga tcggcaataa
    94261 tttccagcgg atcggtctgc ccctgcaaag cagttagctc ttcatgtacc cgcagcataa
    94321 aacggtgaaa gtgcagacgc tgtttccgct ctcccggcag gctttgatag aaaaggtcca
    94381 tcagccaggt tttcccgcgt cctacaccgc cccacatata taagccacgc actggcgtat
    94441 gctttgtgtc ttcgcgttta ccccacagtt taccgacccg cgccattagc ccactcgtcc
    94501 tgggggctgg aggcgtgcta ttgatgagtt cctgataaat aatttccagg cggctgacgg
    94561 cctctttttg aacgtcgtcg ggttgatggc tgccttcatt gagcgccttc aggtattgcg
    94621 atgttggggt aatgctttgc atgatcttat tgttattcct tgaataatcg gtgcgccgtt
    94681 gttcacggtt gacgaaaaaa aggccgttct acactacgcg atatgcagtc gggattccac
    94741 ttctgtggaa ttaacggtta tagtggcata atcagccgca ggcatggagc ctgaagccaa
    94801 caccctacgg aaacaaaaga caacgggaga tgttcatgac ctgggaatat gcgctaattg
    94861 ggttagtcgt cggcatcatt attggtgctg tggccatgcg ttttggtaat cgtaaactac
    94921 gccagcaaca ggcgttgcag tacgaactgg aaaagaataa agctgaactg gacgagtatc
    94981 gcgaagagct ggttagccac tttgcccgca gcgcggaatt actggatacc atggcgcacg
    95041 actatcgcca gctgtatcag catatggcga aaagctccag cagcctgctg ccggaactgt
    95101 ctgctgaagc aaacccgttc cgtaatcgtc tggcagagtc tgaagccagc aacgatcagg
    95161 caccggtgca gatgcctcgc gactattctg aaggtgcatc cggcctgctg cgtactggcg
    95221 cgaagcgcga ctaatttatt tttcgggcgc agccattgcg ccctcctctt ctctccctcc
    95281 ccgactatca tttaatctgg tgtctcattg ttagccgtct gaaaattcaa taacatcaaa
    95341 ctgttttgaa tctcttttct tatcattcag gtacgagagc aggaataatg aaaaaacaaa
    95401 cccagctgtt gagtgcatta gcgttaagtg tcgggttaac tctctcggcg tcatttcagg
    95461 ccgtcgcgtc gattccaggc caggttgccg atcaggcccc cctccccagt ctggctccaa
    95521 tgctggaaaa agtgcttccg gcagtggtga gcgtacgagt ggaaggaacg gccagtcagg
    95581 gacagaaaat cccggaagaa ttcaaaaagt tttttggtga tgatttaccg gatcaacctg
    95641 cacaaccctt cgaaggttta ggctccggtg tcatcatcaa cgccagtaaa ggctatgtgc
    95701 tgactaacaa ccatgtgatt aatcaggcac agaaaatcag tattcagctc aatgatgggc
    95761 gcgagtttga tgcaaaactg attggtagcg atgaccagag cgatatcgcc ctgttgcaaa
    95821 ttcaaaaccc gagcaaatta acgcaaatcg ctattgccga ctccgataaa ttgcgcgtcg
    95881 gtgattttgc cgtggcggtc ggtaacccat ttggccttgg gcaaaccgcc acctctggca
    95941 ttgtgtcagc attaggccgc agtgggttga atcttgaagg tctggaaaac tttatccaga
    96001 cagatgcttc cattaaccgc ggtaactccg gcggtgcact gttaaatctt aacggtgagt
    96061 taattggcat caacacggca atccttgcgc ctggtggcgg gagcgtcggg attggatttg
    96121 ccatccccag taatatggcg cgaacactgg cgcagcagct tatcgacttt ggtgaaatca
    96181 aacgcggtct gttaggcatc aaaggcacag agatgagtgc cgatatcgcc aaagccttca
    96241 accttgacgt gcagcgtggc gcgtttgtca gcgaagtgtt gccaggttct ggctcggcga
    96301 aagcgggcgt caaagcgggc gatattatta ccagcctcaa cggcaaaccg ctgaatagct
    96361 ttgctgagtt gcgctctcgt atcgcaacca ccgagccggg cacgaaagtg aagcttggcc
    96421 tgctgcgtaa cggcaaacca ctggaagtag aagtgacgct tgataccagc acctcttcgt
    96481 cggccagcgc tgaaatgatc acgccagcgc ttgaaggtgc aacgttgagc gatggtcagc
    96541 taaaagatgg cggcaaaggt attaagatcg atgaggttgt caaaggaagc ccagctgctc
    96601 aggctggctt gcaaaaagac gatgtgatca ttggcgtcaa ccgcgatcgg gtgaactcga
    96661 ttgctgaaat gcgtaaagtg ctggcggcaa aaccggccat catcgccctg caaattgtac
    96721 gcggcaatga aagcatctat ctgctgatgc gttaatgtcg taaaccgggc atcaggctga
    96781 cgtgtgatgt ccggttaact cgtggtatgc tgctgccgtt ccctttttta atgacgcctc
    96841 catcatgttt gtgaagctct tacgttccgt tgcgattgga ttaattgtcg gcgctattct
    96901 gctggttgcc atgccttcgc tgcgcagcct taacccgctt tccactccgc aatttgacag
    96961 taccgatgag acgcctgcca gctataatct ggcggttcgc cgcgccgcgc cagcggtggt
    97021 taacgtttac aaccgtggtt tgaacaccaa ctctcacaac cagcttgaga tccgcaccct
    97081 gggatccggt gtaatcatgg atcaacgcgg ttatatcatc accaataaac acgtcatcaa
    97141 cgacgccgat cagatcatcg tcgccttaca ggatggacgt gtatttgaag cattgctggt
    97201 gggatctgac tctctaaccg atctggcggt acttaaaatt aatgccactg gcggtttacc
    97261 taccattcca atcaatgcac gtcgcgtacc gcacattggc gacgtggtac tggcgatcgg
    97321 taacccgtac aacctcgggc agaccattac ccaggggatt attagtgcca cgggtcgaat
    97381 cggtctgaac ccgaccgggc ggcaaaactt cctgcaaaca gatgcttcca ttaaccacgg
    97441 taactctggc ggcgcgctgg tgaactcgct gggcgaactg atgggcatta acacgctgtc
    97501 gtttgataag agtaacgatg gcgaaacgcc ggaaggtatc ggctttgcga ttcctttcca
    97561 gttagcaacc aaaattatgg ataaactgat ccgcgatggt cgcgtgatcc gcggctacat
    97621 tggtatcggc ggacgtgaga tcgcaccact gcacgcgcag ggcggtggta tagatcaact
    97681 gcaagggatc gtggttaatg aagtgtcacc tgacggcccg gcggcgaatg cgggtattca
    97741 ggtcaatgat ttgattattt cggtggataa caaaccggcc atctctgctc tggagacgat
    97801 ggatcaggtg gcggaaattc gccctggctc ggtgatcccg gttgtagtga tgcgtgatga
    97861 taagcagtta acgctgcagg tcaccattca ggaatatccg gcaaccaatt aagtcgtgcg
    97921 ctcaaaacaa aaaaccggag tctgtgctcc ggttttttat tatccgctaa tcaattactt
    97981 attaacgaac tcttcgccca gggcgatatc tttcttcagc gtatccagca taccttccag
    98041 cgcgttcttt tcaaatgcgc tcagagtgcc gatagattta cgctcttcca cgccgttttt
    98101 acccagcagc agcggttgag agaagaaacg tgcgtactga ccgtcacctt caacgtaggc
    98161 acattcgaca acgccttgtt caccttgcag tgcgcgaacc agagacaggc caaaacgtgc
    98221 agctgcctgg cccatagaca gggttgcaga cccgccaccg gctttcgctt caaccacctc
    98281 agtacccgcg ttctggatac gtttggtcag atcagccact tcctgctcgg taaagctaac
    98341 gccaggaacc tgtgacagca gcggcagaat ggtaacacca gagtgaccgc caataaccgg
    98401 cacttcaact tcgcctggct gtttgccttt cagttccgca acaaaggtgt tggaacgaat
    98461 gatatccagc gtggtaacgc cgaacagttt gtttttgtca taaacaccgg cttttttcag
    98521 cacttcagca gcaatcgcaa ctgtggtgtt aaccgggtta gtgataatac caatgcacgc
    98581 tttcgggcag gttttcgcaa cttgctgtac caggtttttc acgatgccgg cgttaacgtt
    98641 aaacaggtcg gaacgatcca tacccggttt acgcgctaca cctgcagaga taagaacgac
    98701 atctgcgcct tccagcgccg gagtcgcatc ttcaccagaa aaacctttga ttttcacagc
    98761 agtagggata tggctcagat cgacagccac accgggagtc actggagcga tatcatacag
    98821 agagagttct gaacctgaag gcagttgggt ttttaacagt agtgcaagcg cctggccaat
    98881 accgccagca gcgccgagga ctgcgacttt catcctaaac tccttattat attgataaac
    98941 taagatatgt tgctccgctg ccgcgacctt aatccacaaa attgccgttt acaatgacca
    99001 catctcaaga atgtgtagtc acgcaagttt agcgtttatg catttaattg ccgtaatcag
    99061 gaacctaact tacgtaatta acagtcgctt atgcattagc gcaacattcc aacaggtggt
    99121 gacaatatac cctaccgttc agccaaaaca acatcaattt gataacaatt aatttacttt
    99181 taagcagaat ttgcatgccg tgacgcaggc atgtttctca ataacgaaat ttgataaaat
    99241 cccgctcttt cataacatta tttcagcctt cttcagggct gactgtttgc ataaaaattc
    99301 atctgtatgc acaataatgt tgtatcaacc accatatcgg gtgacttatg cgaagctcgg
    99361 ctaagcaaga agaactagtt aaagcattta aagcattact taaagaagag aaatttagct
    99421 cccagggcga aatcgtcgcc gcgttgcagg agcaaggctt tgacaatatt aatcagtcta
    99481 aagtctcgcg gatgttgacc aagtttggtg ctgtacgtac acgcaatgcc aaaatggaaa
    99541 tggtttactg cctgccagct gaactgggtg taccaactac ctccagtcca ttgaagaatc
    99601 tggtgctgga tatcgactac aacgatgcag ttgtcgtgat tcataccagc cctggcgcgg
    99661 cgcagttaat tgctcgcctg ctggactcac tgggcaaagc agaaggtatt ctgggcacca
    99721 tcgctggcga tgacaccatc tttactaccc ctgctaacgg tttcaccgtc aaagacctgt
    99781 acgaagcgat tttagagctg ttcgaccagg agctttaatc tctgccccgt cgcttctggc
    99841 ggcggggaaa atgttgctta tccctctcaa ccccctgctt tcctctccga ttaatttaac
    99901 gaatagtgcg ttttactgcg acatgtcatt cacacaatga atacatacgg taaaaaaagc
    99961 acattatgca aaattcatta tctaattgaa aacaatagaa ttaataataa ataaccgtgt
   100021 ttttaattct tttttgttat taaaactcac atttttaaca cttagtatca actgaaacag
   100081 ttagcgtggt attaattagt tcaataatta gtgtatactt gattttgtga tatgggtcac
   100141 gaaacaaagg cccagctaaa agattatgtc gaggtaaaaa tcatgaaaat caaaaccact
   100201 gttgctgcat taagcgtact ttctgttctc tctttcggtg cattcgctgc cgactccatt
   100261 gatgctgcac aagcacaaaa tcgtgaagca atcgggaccg tatccgtaag tggtgtggcg
   100321 tcttcgccaa tggatatgcg tgaaatgctg aacaaaaaag cggaagagaa aggcgcaacg
   100381 gcctaccaga ttactgaagc tcgtagcggt gacacctggc acgctacggc tgaactgtac
   100441 aaataaaccc tcatcgtctt gtccgacgat attgcccccg gttcgggggc ttttttttgc
   100501 gctaatgacg aacattaaaa cgcaaatgcc cttccagctc ttcctctgcc tcatcaaaca
   100561 gcaatattaa cgcgccaaaa cggcgacgcg ttttctctcc cagatgaaca aattcaatct
   100621 caagtggtag cggcaggaca tcgttcatta acacatccca tagtgagtcg agatcgcgta
   100681 ccttatcttt cgccagacca aaggcttggc tgaagtcgcg ataaaaatcc tcctgactct
   100741 caatctcatc aaaatcaaag gtataaatat tcatctgttg ccaccgtcac gtttcgcggc
   100801 gagttctgcc gttcatcgct aagtatagcc atgaaaaaac cgacgctttt ggcgtcggtt
   100861 ttgacttaac tatcggtcaa cgcatgttga tacttatgga gcatccccgc cagccgatga
   100921 accggttccg tcacctgcgg tggcgcttgc cagatgcgta gcttttcctg atagatttca
   100981 agttcttcca gcaactggcc aaagtaccga cgacgtttat catcgctacg ggcagatatc
   101041 acatggtctg ctgtgcggcg catttgtcgg tgaaacgccg ataaatcctc gttaaccggg
   101101 atcggcgcat cacgcaggcg ctggtgcgcg ataatcatcg tcagcgccag gcgaaatttc
   101161 ggcaaatccc ctgggaactt attcatcagc aaaaacagct gctgataaag tgccgggagg
   101221 tggttctctt tacgacgtgc cacattggta gtcatcgcgg aaacagcagc agaaacgaac
   101281 tgattaagca gtacacgtcc agtcctgtcg cgcgatttat cccgcaccag caaaataacg
   101341 gtgaacgcga gcacacagcc gacgatttgc cctaatgcgc tgtcgagaaa ctgactgaaa
   101401 tggaaagtca tcgggttatc cagcacgata atatttatgg tgctggccag cgcccccatc
   101461 gagcccagtc gccgtttctg tacttctata ccgaggaaga atcccagcac tgccaggcta
   101521 atacacagca gcaacatgct ctgttgagta ttagggataa tcaccaaaaa gtagagcagc
   101581 cctaacggca gcgctgccag cgtcccgtag ataaagtcga tcgccaccat gcgtggattc
   101641 ggcaaacgca ttgccagtga cgtcactacc gcaatcatca ccattgcacc actgccggaa
   101701 gtccagcccg tccacagcca gaaaagcgtc cccagaatgc aggaaagtgt ggttcgccag
   101761 aagttaacca tcgcatgatg acgttcggct gactctactt tgacttccgg ttcgccttgc
   101821 aggatctctt cttcggtggc gttgattttt gtgttactga taacgccgcg cttgagaagc
   101881 tgataacgcg ttgccgccgc gacccagcta taaatggtga caggcgtttc ccgttcccca
   101941 gtccaggcga taactctccg caggcgtttg agctgcttgt ggacgtcctg cgcggtttct
   102001 accggcgtgt caaaaaattc gcggaaagta tcagtgatca attccgggcg cgtattctga
   102061 ataagataag tttcgcagga ttgtgtaatc agcgtcagcg atagcgtatt aatcgctttt
   102121 aaacgtcgat tggcccgcgc ccagcgggaa gattccatat tcaggttgct gcgcatacct
   102181 tgtaacgccg tggtgcgtcg caccagatcg ccccaggctt tatcgacaac ttcaccatcg
   102241 ccatgcttga tacagagttg cattaattga tattgcgcga ccagcaaact ttccagctct
   102301 cgatccactt cttgtttgat cgatcgcgga gaaaagagca aatccgccat aatggcacac
   102361 acaataccga taacgatctc gctacaacgt tcgacggcaa actgcggcgt aagcaatggt
   102421 tccggctgaa tggtgatcac aatgatcagc gcggtataac cggccagccc ccacgcatac
   102481 gagttttcta ttcgtaccag cgaggatatc caggtacaaa aaccggccca gatacagcac
   102541 accagaatca tcaataatgg tgcacggatc atcgcaataa tgatcaccag tccggcaata
   102601 cagccaataa atgtgccgat gatgcgcaaa aagccacgat agcgaatagc gccagaatac
   102661 ggttcacctc ccgcagcaaa ggccggaccg gcggcaacaa tcgccgctgt cagtactgcc
   102721 cagcgtggcg tttccagctg gaagtgaaag ccaacaaaca gcgccagtac aatggcggtc
   102781 gccagtttta ccgcaaagcg aatatgttgg ttagcaatgg agaaaatacc catcgtgatt
   102841 aaccaaactc acgcaggcga tgggccattt tacggaagaa cgaatcctgg ctttcgtcgc
   102901 gatcttgttt gccagtgacc accactgtag cagtggtgcc cgcaggccag atgttctctt
   102961 gctggttgtc gagacgaata cgaaccggaa cacgttgcgc caaacgcacc cattcaaggt
   103021 tagagtcgat ggtcgccatc cctttgtcgt cacgcgtgct gctggcgttg gtgacccctg
   103081 cggcaacact atcaacagtc ccttttagca ctttgttact gccaagcggc gtgatctctg
   103141 cacgataccc agggcgcacc ccttccagct tagtttcttc catataggcc agtacataga
   103201 aggagttctg tttcaccagc gcaaccgccg ttgatccacg ggtaataaac tcaccggtat
   103261 agacgttgag gttggtcacc cagccatctg ctggcgcgcg gatcaccgtg cgttcaagat
   103321 ccagttttgc cagatcgcgg gtcgcctgcg ctttcgctaa ctgatgcaga actgtttgta
   103381 gtacgttgtt ggcctggtcg atctcttcgc gggacatcgc ctgcacaccg agacggttac
   103441 gacgtcctgc ctcctggcgt ttctcctgtg ccagtacctg ataataagca acatcggctt
   103501 gcgcttcctc aagcgccttt tgatagcgcg gctggtcgat ggtgaacagt acctgtcctt
   103561 ttttcaccag ctggttatca tgaacattca cctgggtgat aagtccagaa acgtccggcg
   103621 cgatcgcaac gacgtcagcg ctaaagcgcg cgtcacgcgt ccacggggat tcggtgtaat
   103681 agacccaggc attaaaaatt gcgatgaagg ccagaatgac taatacgacc gtgatggccg
   103741 tacgggagaa ttttcttatt agtgttttca cttcaacctc aaacgaacag tcgcgatatc
   103801 aaataaaaca agcagcaata gagcgcggtg ttgaacaacg ccggatgcca gacaaagtcg
   103861 tagatacctg ttggcacaag tacccggcgc accagccaga aaatcgccag tgataaaagc
   103921 aattcaaaaa atatcggtgg gaaggacagc ccaaacacca cgataacggg aaacagactc
   103981 atgttgacct tggttgtata gagagagcag gcgttattat tttcagcatc tgtcgccgca
   104041 gagaagggca tggaaagccg ggcgagagca acattgctgt agattgatat ttaatatatt
   104101 agcgtaactg ttatgctgtt atctatatta tgtgatctaa atcactttta agtcagagtg
   104161 aataatggaa cgactaaaac gcatgtcggt gtttgccaaa gtagttgaat ttggctcttt
   104221 taccgccgcc gccagacagc tacagatgag cgtttcgtcc atcagtcaga cggtgtcaaa
   104281 actggaagat gagttgcagg taaagctgtt aaaccgtagc acacgcagca ttggcctgac
   104341 cgaagccggt agaatttact accagggctg ccgtcggatg ctccatgaag tgcaggatgt
   104401 tcatgagcaa ctgtatgcct tcaataacac ccccatcggg acgctgcgca ttggctgttc
   104461 ttcaactatg gcacaaaatg ttctcgccgg gctgacagca aaaatgctga aagaataccc
   104521 aggtttgagc gtcaatctgg ttaccggaat tcctgccccc gacctgattg ccgacggtct
   104581 ggatgtggtg atccgcgtcg gcgcgttgca ggattccagc ctgttttccc gccgtctggg
   104641 cgcgatgcca atggtggtgt gcgccgcgaa aagctatctc acacaatacg gcataccgga
   104701 aaaacccgcc gatttaagta gtcattcatg gcttgaatac agcgtgcgac ccgacaatga
   104761 atttgaactg atcgcaccgg aagggatctc gactcgcctg atcccgcaag ggagatttgt
   104821 gactaatgat ccgatgacgc tggtgcgctg gctgactgca ggcgctggga tcgcctatgt
   104881 gccgctgatg tgggtgatca acgagatcaa tcgcggggag ctggagatcc tgctgccgcg
   104941 ttaccagtca gatccacgcc cggtttatgc gttgtatacc gaaaaagata agctgccgct
   105001 gaaggtacag gtcgtgatca actcactgac ggattatttt gttgaggtcg gtaaattgtt
   105061 tcaggaaatg cacgggcgcg ggaaagagaa gtaattcata ttgtactgtt acgttgtaca
   105121 aacctgtgcc aatgggttcc cctcacccta accccagagg gggaggggac cgttcgtgca
   105181 cgtagaagga ttaattatcc ttctgaaaat actgaaatta ttacgcggta ccgccaacag
   105241 tcaggttatc aactttcagc gttggctggc ccacgccaac cggcaaactt tgcccttctt
   105301 taccgcagac acccacgccg ttatccagtt tcaggtcgtt gccaaccatc gaaatctgct
   105361 gcatggtttc gataccggaa ccaatcaacg tcgcgccttt caccggcttc gttactttac
   105421 cgttttcaat cagatacgct tctgaagtgg agaaaacgaa tttgccggag gtgatatcca
   105481 cctgaccgcc accaaagttc ggtgcataga taccgtactc aacggattca ataatttcct
   105541 gcggggtcga tttacccggc agcatatagg tgttggtcat acgcggcatc ggcagatgag
   105601 cgtaggattc acggcgaccg ttgccagtcg gcgtcatccc catcaaacgc gcgttgagtt
   105661 tatcctgcat gtagcctttc aggatgccat tctcaatcag cacgttgtac tggcctggcg
   105721 taccttcgtc atcaatcgcc accgaaccac ggcgatcgac catcgtgccg tcatcaacca
   105781 cggtacacag ttcagaagcc accagctccc cgacctgtcc actaaatact gaagtgccac
   105841 ggcggttgaa gtcgccttcc agaccgtgcc ccaccgcttc atgcaacagc acgcccggcc
   105901 aacctgcgcc aagtactacc ggcatggtgc ccgctggtgc agcaacggca gaaagattga
   105961 ccagcgccat acgcacagct tcttttgccc atgcatccgc acggacttca ccgtcgagat
   106021 cggcaaggaa gaactcataa ccaaaacgac cgccgccgcc actggcaccg cgttcgcgtt
   106081 tgccatcttc ttcgaccaga acgctcacgg aaagacgcac cagcggacgg acatccgccg
   106141 ctagcgtgcc gtcggtggcc gcaaccagaa ttaattcata gacaccactg aggctggcag
   106201 tcacttcctg tacgcgcttg tccgcttcgc gggcaacctt atcgacgcga cgcaggatat
   106261 ccagcttctc ttcacggctc atgctttgca gcggatctac cgaggtatac aacgggctat
   106321 gctctaccgc gcccagcgtc tgtactttgc catcaccact atcacggacg atggtgcgcg
   106381 ccgcttgcgc actctgttcc agcgccagca ggctgatttg gtctgcgtaa gcaaatccgg
   106441 ttttttcacc gctgattgca cgcacaccaa cgccctggtc gatgttgtaa gaaccatctt
   106501 taataatgcg gtcttctaaa acccaggatt cgtgatagct cgactgaaaa tagagatcgc
   106561 cataatcaag gcgacgttcg gccagttgac cgaggatcgc gaacaagtcc tgatgtttca
   106621 ggccgttcgc cgctagcaat tgttcactta ccaggttaag actcatcgtt tttgctactc
   106681 gttagttact gcagtagagg attttttacg gctgccggat gcggcgtgaa cgccctgtcc
   106741 ggcctacggt tatgttctgg ctcgttggcc tttggcaacg attatcatat gagattgggg
   106801 caattacgcg ccctcgtcaa atcattgcgc tttttcttta cgcggttggc gcaacacttc
   106861 gttgatttgc ggatcgtcca gcggacccga aatgtgatag cgcaaaatgg agactttgct
   106921 ccacagcggc cccagcactt tactggctgc aaacactgcc gcgccaacaa tggggttaac
   106981 cgcaaaagcc gcagccacgc ccaccgtcgc agaaatctct ggtgcgacaa ccgcttccat
   107041 attcaggtcg cgacgcacca gatttaccga ccctttcatg gcaatgtccg cttccaggcc
   107101 atccaccagc gtgtcgtcgg tgtgcataac gccgtcttta atccacgcgg tgctgcgaat
   107161 ggagtcaaaa tagaaccctt caccaaaggt gtctctgaaa tcaaaacgca gcttacgcat
   107221 cagggcatca acgctcaata agcgcagcaa ttgcccggca tgtccggtat tgatttcggt
   107281 aatttcgcct ttgcccagtt gagtatgaat gatgccattc aacgtcgcct catctggttg
   107341 ccagggtgct ttgcgccagt gtaaatcgta atccacatta aatgacgact ggcgtatggg
   107401 cgtcgtgaca ccaaaaaatt ctgcggcggc atcaattttc tgcccacgca gttttccttt
   107461 cagcgaggtg cgttcatttc ccggattgtt aacccattca ccatcggcag tgagccgcga
   107521 aaaaccagta tcaatcagtc cattagtcag cgttaatgta ttgccagaaa tggtgatatc
   107581 actgtcaatg cgcccgaatt tttgccccca gaaccagcac tctgcgcatc gtatttgggc
   107641 gtccggccag ccacggaagt taatgcgctc cgttgtcggg aaaggtgatg actgtgttga
   107701 atcaccacga gttttcgcca cgctcgggtt gtaataaaga tatttgatat tcgccagcca
   107761 cggcgcgtta ttacgcatcg ccagcgtggc gttgatttca cgcccttgcg cctcaacctg
   107821 ggtgccattt gccgtcggtt gcgaaacaat actcaggtta ttccattgct gatttcccag
   107881 cgacaacata ggcgtacgta acgttatgtg ttgtgggaaa ctcgctgcac caccgacact
   107941 ctccgccgcg cctttctgga acagggccag ccactcggca ccattcatcg gcggcatatt
   108001 gagttcaacg ccactttgtt ccggcaacgg cgggagcgtt ttactgtctg ccgcccaaat
   108061 agcacgatcg agcgtcaact tttgaccgag caaccagcgg ctattgaaat ggttatccgc
   108121 acctgcctgt ccggttaatt caaagctgtt gagattgcca tcaaccttaa cgtttaccgc
   108181 tagtggttca cccgcaggtt tggctaacgg tgaaggtaag tgactgctca cattcttcag
   108241 gtcgccgttc agctctacgt tataggtcgc gccagcatga taaggcagat caatgcccgc
   108301 tttaccatcc cacgccacgc tgccacttaa tgcttcgttc accgctgcag gcagaacgcc
   108361 ggttttcgct ggttgccagt taccgttgag attcaccgca acctggtagg cttttgcccc
   108421 ttctttggtg gaaaaatcca cgttcaacgg ctgattaaac cagcttgctg tcagtggttc
   108481 actttgcaaa tcgccattga taaagctgaa tttaccgctc aaatttttca gggtgctgtc
   108541 gagtggtttg ataaacagac tgttattacg cagcgtcact tcacctttcg cggttaccag
   108601 ttcgccgttc agcggaatat cgagatgtaa gcgagcattc acatcgccgt cgagctggag
   108661 ttcttgcagg gttgcaccca gagaatcttt cagcggtgtc tcatcaaagt aagggccgac
   108721 ggctttaccc ggacctttaa tgtcagcgtc aatcagcagt ttttctttcg agtagtcagg
   108781 gatcactgcg gtgagattac tcgcgcgcac gccgcccaga ttaacgccat cggttttcat
   108841 ccataaaccg tcgttaataa agtccagttc aatatcaagg ttagttaatg caggccagtc
   108901 cggctggaag gcaaacttcg cgttgcgcag cggcaccagc acttcaaact gaccttcgtt
   108961 gtgtttatag gggaagagtt gcggattgcc accataaacc agcgtcgcgt tatccgctac
   109021 accgccctga atcgcgccac ttaagtaatc aaccaggtct ttacccatca agttttccgg
   109081 gaaatagcgc caggcttgtg aaccatcatc ggtactgatg ccagccagaa tacccagcca
   109141 gggttcatcg ttagcaggtt gcaggtaacg aaaaccgccg cgcgcatgga cggctttggc
   109201 tttaacgtca atattacgcc catccagctg gaaacctttg tcattgttca gccagcttat
   109261 cgttgcctgg ccgtcggcga tttccagtgg cgcacggaat accgtttcgt aaggcatctt
   109321 tgcctgcttc atcgacgcgg taagcaaacc attttctacg ctgccggaaa gcgtcccgga
   109381 gaagtgttcc gtacccggta ataatttcca ttgattccag gccagatcgc tccacgatgc
   109441 ctgaaaacgg gtcttgtctg ccgcctgaag cgggatatcc agcgccagag tgttaatctt
   109501 gccgctcggt tgtgtggagc gccaaacatc acccagtgca ggtgaaagtt tcgcggccag
   109561 cgggcgtatg ccctccaggc ctgccagctc cagattactg gcgcgaatcc ggagttcgtc
   109621 actgcgttta ttgtctttgc cgccaacgtc ctgttccggt atccaggcca gcgtcaatgc
   109681 tccgctcggc cagggtttgc cgtccatcgt gatccgtgta tcgggaatag agaactgcca
   109741 gcctggattt tcacgcgtaa tatgcgcggt cagattatcc accgacagcg tatgcgtttg
   109801 cttctcgccc aaccagctgg caccgccctg tttcagccag acgtcaccgc cggttacatc
   109861 gcctttgtcg atcgtcatcc agccttcaag ggagaactgt gccgtttcca gcgcaatatt
   109921 gtcctgcatc catttaccga gccacggctt caggtcgatg tcatccgcct ggagccagac
   109981 gcgaccattg cttaacaacc cctcatcatc gcgcaaatcc atgcgcacct gcatcacgcc
   110041 gtgctgtccg gtaaggctgg agaggcttac caggccttct gcacggtgtc gacgtggatc
   110101 gttcagccag gtgagttgtg ggatcgccag ctcggcgcgc tgaccggatg gcgtcaggaa
   110161 actgacttca ctgtcgcgga gatcaaaatg gtcgaactga cgaagaaaca gatcgctgat
   110221 gtgactggct tccaggctat cattaccacc gccgctggtg ataggagtgt tggtgcgaaa
   110281 gcgcagctgc cagaaagtga ggtcgcgaaa ctgccagcgc atatgtaaca ggctctgcca
   110341 gacatctagc gcaagagtaa cgcgtttaac cgaaaattcg ccgccatcct ttagttctgc
   110401 acggatgtcg tgtgcttcaa gcgtcgggcc aaaattctgc cagctggctg agagctgact
   110461 ggcttctacc ggcatgccag tcgcggattc tattttgttg aggatttccg gacgccaggc
   110521 atcaagatgc ggtaaagcaa tacgcaggcc gctaaccagc agggcagcga tcacaacgag
   110581 cgcggctcca gtaagcagta atatccccgg caatcgcctc acccgtcact ccttgtctgc
   110641 taaaaatgtg actcaaaaac cctttgccgg atggcggccc agcatctgtt tacatcatta
   110701 cgacgtcaaa ctgctcctgg ttatagagcg gttcaatttg tactttaacc tgtttgccaa
   110761 cgaaaatttc cacttccgcc agcgagtgtg actcttcacc tttcaaggct tcagctactg
   110821 ccggagaagc atagaccagg aaacggtcgg aatcataagc atggtgtaca cgaacaatct
   110881 cgcgcatgat ttcatagcat accgtttcca cggttttcac cgttccgcga ccgtggcagg
   110941 ttgggcattc gttacatagt acgtgctcaa tgctttcgcg ggtgcgttta cgcgtcatct
   111001 ccaccagccc cagcgccgaa aaaccattaa cgctggtttt cacccggtct ttgctcaacg
   111061 cctgctccag cgaatgcaac acgcggcgac ggtgatcttc attattcatg tcgatgaaat
   111121 caataatgat aatcccgccc agattgcgca accgtaactg gcgagcgata gcctgcgtcg
   111181 cttcaatatt ggtattgaaa atggtgtcgt ccagattgcg atgaccgaca aatgctccgg
   111241 tattgatgtc cacggtggtc atcgcttcgg tctggtcgat aatcagatag ccaccggatt
   111301 tcagttctac tttgcgttcc agcgcccgct ggatttcgtt ttcgacatca aagagatcga
   111361 aaatcggctg gcgtccggtg taatgctcca gcttgctggt catctcggga atgtactccg
   111421 aggtaaactc cagcaatgct tcgtaagtca ggcgtgagtc aacgcgaatg cggtcgagtt
   111481 cggcgtcggc gaaatcacgc agaacacgct gcgccagcgc cagttcgcca tacagctgat
   111541 aacgggtctg cgggcgtttt ttacgctcca taactttggt ccagacgcgt ttcagataag
   111601 cggcatcgga ggccagttca gcctcgccaa ccccttcagc tgcggtacgg atgataaacc
   111661 cgccctgctc gtcgcaatac tctgcgacca cttttttcag gcgttcacgt tctgattcgc
   111721 tttcaatacg ttgggaaacc ccaacgtgag aagcccctgg cataaacacc agatagcgag
   111781 aaggcagcgt gatatcggta gtcaggcgcg cacctttagt gccaagcgga tctttcacca
   111841 cctgcaccat cagatcttgc ccctgacgga ccagttccga gatgtcgcgt acagtgaatt
   111901 gcttttgttc ttcacccgcc acacattcag tgtgcggcat gatatcggat gcatgaagaa
   111961 acgcggcttt atccagccca atatctacaa aagccgcctg catacccgga agtacacgac
   112021 ttacacgacc cttgtagata ttgcctacta ttccgcgtcg cgcctcacgt tcaatatgaa
   112081 tttcctgcag aataccgcca tcaatatacg ccactcgcgt ttccgaaggc gttacgttta
   112141 ctaacaattc agccgtcatg tttatccctt ttctcacgca gtgcgttaaa attacttaat
   112201 aattcatacg tttcaaccag cggtaagccg actacggcgt gatagctgcc atttatcttc
   112261 ctgacaaaac agccacccag cccctgaata ccgtatgcac ctgctttatc taacggttca
   112321 ccgctggcga catagcccgc gatgtcttcg tctgttaacg ttctgaaagt cacatcggtg
   112381 accaccaggc aatcgagaat gtgctggctg tcggccaacg ccactgccgt cattacctga
   112441 tgggtctggc ccgataattt gcgcaacatc tgcaccgcat gctctgcgtc gcgcggtttc
   112501 tccagcactt ctccgttcag gataacgata gtatccgcac ccagcaccgg gagatcctgc
   112561 gccgtttgcg cgacacccgc ctgtgctttc tcgcgcgcca gacgcacaac atactgctgc
   112621 gcgctctcct gcggctgacg ctgctcctca atgcccgtaa caatacgttc aaaggttacg
   112681 ccaagttgcg caagtaactc ctgacgacgc ggagaaccgg aagctaaata cagagaagtc
   112741 atagaaacct ttattgcact gcaaactgct gacggacttt acgcatcagc aagaaaatcc
   112801 acggccagag cactccattg actacactac tccagaacac ttccggtctg aaagagacgt
   112861 taatcactaa aaactctgcc cagaaaacaa taatatccac caccagcgaa agcaacatga
   112921 cgaccagcgc ctgctgccat aatgcgaggt tgcggaaaag ctgatatttc agcgccacca
   112981 ggtaagcaat gatgctcatc gccaatacgc gtacgccaag tgtcgagccg ctgatcagat
   113041 ccagtatggc acccatcaca aaacctgtgc ccacatttac gcgatgaggc aaggccagga
   113101 tccaatacaa caagatgagt aagacccagt ttggccggaa aacaatcagg ttatccggcc
   113161 agggcatgat ttgcagcaac agcgcaatga ggaaagagag ccagattacc cagcgtccct
   113221 ggctacgata gctcgccact attgccctcc cggcgcacgc gcaggcggtt gagcaccact
   113281 ttgcggcggc gtagcccttt gtggagagcg attagcagcg ggctgtgtcg gcgcagcagg
   113341 cgcagtagct gcatttcctg tcgccggttg ctgcggagtc ggctgagcga tccccgttgc
   113401 cggttcaggt aactttggcc ccatcgcgtc tggcgaaggc aatacctgcg gcatcatctg
   113461 catcagacgt tcattagcaa cacgatgcac ctcttccggc gtcatcgggt tagcgccgtt
   113521 acgatccgcg ccccacagca gcagcagata acgcaaacgt tgcagccctg cagtcggacg
   113581 cgcctgaatc acagtataag cgcgctgggt atcgagtttt acggaagaga caaccgcgac
   113641 cggatagcct tccgggaaac gaccgcccag accggaagtc accagcacat caccaacacg
   113701 aatatccgta ttcgctggca gatgctcaag ctgcaaatca tccgtacaac cgttaccggc
   113761 tgcaattacg cggatatcgt tgcgcagcac ctggattggc agcgcgtggg tcgcatcaca
   113821 aatcagcagc acgcgactgg tcagtttagc gacggccacc acctgaccaa caacaccttt
   113881 gtcgctgatg accggctggc cttcataaac gccattaacg ctacctttat cgataacaac
   113941 ttgatcgcta taaggatcgt taaccgtgga gataacctga gtcaccattt tctgctcatc
   114001 ctgacgcagc ggggaaccca gcagttcgcg cagacgcgcg ttctcctgtt tgtattgtcc
   114061 aagcatcagc agttcactgt ttttcagcaa cagttcctga cgtaacgccc ggttttcaag
   114121 ttctagttgg tcacgcgagg ccagcgtctg cgatacgcca tccagcaatt cacgaggagc
   114181 attggaaaca aagtagaaag gactgacggc ggtatccata taagtacgga tttgactgaa
   114241 cgtccccagg cggctgtcgg caataataat gccgagcgcc accagcaccg ccagaataag
   114301 gcgaatctgt agcgacgggc cacggctaaa aattggcttc ataagttatg cgtattctcg
   114361 tatcagacca ggcagggtaa acagacgctt cccctgcctg catccgatta ctcttcgctg
   114421 aacaggtcgc cgccgtgcat gtcgatcatt tccagcgctt tgccgccacc gcgcgccaca
   114481 caggtcagcg ggtcttcagc aacaacgact ggaatgccgg tttcttccat taacaaacgg
   114541 tcaaggttac gcagcagtgc gccaccaccg gtgagcacca tgccgcgctc ggagatgtcg
   114601 gaagccagtt ccggcgggca ctgttccagt gcaaccatta ccgcgctcac aataccggtc
   114661 agcggttcct gcagtgcttc gaggatttcg ttggagttca gggtaaaacc gcgtggaaca
   114721 ccttctgcca ggttacggcc acgaacttcg atttcacgga cttcatcgcc cgggtaagcc
   114781 gaaccgattt cgtgcttgat acgttctgcg gtggcttcac cgatcagaga accgtaatta
   114841 cgacgcacat agttgatgat agcttcgtcg aaacggtcac caccaatgcg cacggaagag
   114901 gagtaaacca cgccgttcaa ggagataaca gcaacttcag tggtaccacc accgatatca
   114961 accaccatag aaccggtcgc ttcagaaacc ggcaggccag caccaattgc ggcagccatc
   115021 ggttcttcaa tcaggaagac ttcacgggca ccagcgccct gcgcggattc acgaattgcg
   115081 cggcgttcaa cctgggtcgc gccaaccggc acacaaacca gaacgcgcgg gcttggacgc
   115141 ataaagctgt tgctgtgcac ttgtttgatg aagtgctgga gcattttttc agtcacgaag
   115201 aagtcggcga taacgccgtc tttcattggg cgaatagcag caatattgcc cggcgtacgg
   115261 cccagcatct gcttcgcgtc atgacctact gcagctacgc ttttcggtga accggcacga
   115321 tcctgacgaa tggccaccac ggaaggctca ttcaatacga tgccttgtcc ttttacataa
   115381 atgagggtat tcgcagtacc caggtcaatg gacaagtcat tggaaaacat gccacgaaat
   115441 tttttcaaca tactaaggga taatcctgaa agctggggcg gaaaagaaaa tccgcttact
   115501 ttaccaacca cacgcagcag cgacaaggcg caaaaatcat ctgctacggt gaaaattagt
   115561 gcagttcgtt tcctttgtta caaatctctg cctgagtcca gaaaggctta atgcatcagc
   115621 agcattcctc gcctgtttgc aaccgcgtaa ggtcattcat ctgcatatgt gctgcaacaa
   115681 tctggcgagc agacaagcac actcccatga gacgcagcgc gcgttattct acgtgaaaac
   115741 ggattaaacg gcaggttaaa ccgagtatct ttgtgaatat tttttcacgt tagtatcaag
   115801 tggctgtgag gacgcgaaaa aatccccttg cccacctgta acaccgcgct gaatcagggt
   115861 ctgccactcg cttcgcgaac gcacgccggt ggcgtaaacc tgggtgctgg tcccggagca
   115921 ggcttccacc aggctttgaa ccagcagctg gttctccgtt cgcttctcaa tgtttctgac
   115981 cagccccgga tggagcttga gtaactcaac attaagttct ttgatccaac tggtgctaac
   116041 cagcgtcaaa ccagcctggt tgacggctac ccgtacccct aaagcattca ctaaacgaat
   116101 aacaggttgt aaacgactga tatgttgacc tacatcggcc tctgcaagtt caataattat
   116161 gcgttttcgt tgtgattttt cacattgcat taacgtatcg cgcagccaac gctgaaaacg
   116221 cgggcgaatc agcgactcaa cggtaacctg aatcgccaga ttttcctccg gccagtaacg
   116281 taatagtgga ataagacggc tgatttgcag acggtcatac tcttccgata agccaaactg
   116341 caagaccatc ggcatatact ccgccgagct aacctcttca ttaccatcga agatgcggca
   116401 catgagttcg cgatgatgaa cctgaccttc gcgagtaacc gccggttttt gataaaggcg
   116461 cgggccgccg cgactcagca tttgctcgat aagcgtacgc cagcgaacat taccgcgtcc
   116521 tttttcaggc aacgagtcat cgtaaatagc ccagctattg ccgccctgca atcccgcatt
   116581 acgcgtggca gactctgcat gttccattac ctgctcggta tcctgaccac tacgccaggc
   116641 acagatacca atgtggatca tatcgtcgcg atcgagcatt ttattattcg gcaaggtatc
   116701 gacggctttg attaactgac tggctatgct ctcagcctct tttaacgtcc ggtgcggcaa
   116761 cagcgcagca aaatcactgc ggtggtaacg cgccagcagt gcgccagggt agcgcatcat
   116821 aaatgtcgac agcagattcg tcagagagaa gaactgttct tcaacctggc tgtgccccca
   116881 ggtatcactc aacatattga aatccggcag acgaatcatc atcacgatcc cgtgggtacc
   116941 tactttctcc tgatcttcca gtaacgttgc taactgatta tcgaagaaga gtcggttatt
   117001 gaggccggtt ttcatgtcct gggcggcata agagcggatc agcgtatcaa gacggctgtg
   117061 ttgttcacgt gcgttctgaa tttcacgaag cagcgtatcc agcgcactgc tggttctggg
   117121 cggccattca tagatggttc ccaacacatt agagccacgc tcaccgttta agatacgagt
   117181 agcccgggtt tccagcaatt cttgcccggc aagttgccgt tgtaaccagc gtaccgccag
   117241 gaagagcata agaatgataa agccaatcgc ccccgtgagc ggcgcggtgg tcatcaacga
   117301 atggaaatag ttgcccatcg gatcctgata aaccagacgc agcgacatcc ccggatgctt
   117361 tatcaacgga acgctcagtt cgcgaaacag atcgctggtg ccaaccggac gataactacc
   117421 atttctggcg agggtataaa cctgtttatc accatgcagc aggtctacac gaacgatatc
   117481 tgccgacatc attaattcgg taatttgtgg ccttaatgcg ctgaagtcat tcgacacaag
   117541 atgggtatca atcgccgtcg ccaccgcctg aacgcgatga ctaaacttat actgaatggc
   117601 gttgtagaaa cttagcgaac agcccagcaa agtcacaaaa attgttaacc cggtgagcag
   117661 cgtaacaaag gccgaaaatt tcgtcgttaa tctcatcctt gtgttaactc cgatagtgag
   117721 gaagcgggca tactagcaaa tcagatttat ctcgcaattt attgcgcttc atcggctttg
   117781 cttttccatt agcgagtata gtcttcagaa attattttcc aatccatcat gcacatgagg
   117841 accacttatg caggcgttac ttttagaaca gcaggacggc aaaactctcg catcggtaca
   117901 gactctggac gaaagttgcc tgccggaggg cgatgtcacg gttgatgttc actggtcgag
   117961 cctgaactac aaagatgcgc tggcgattac cggtaaggga aaaatcatcc gtaattttcc
   118021 gatgattcct gggatcgatt ttgccggaac tgtacgcacc agcgaagatc cgcgttttca
   118081 tgccggtcag gaggtgttac tcactggctg gggcgttggt gaaaaccact ggggggggct
   118141 ggcggaacag gcgcgagtga aaggtgactg gctggttgcc atgccgcaag ggctggacgc
   118201 gcgtaaagca atgattatcg gtactgccgg ttttaccgcc atgctgtgtg tgatggcgct
   118261 ggaagatgcc ggtgttcgcc cgcaggatgg ggagattgtc gtgacgggtg ccagtggtgg
   118321 cgtcggcagt accgccgtgg cgctgctgca taagttgggt tatcaggtcg ttgccgtttc
   118381 tggtcgcgaa agcacccatg aatatctgaa aagtttagga gccagccgta ttctccctcg
   118441 cgatgagttt gccgaatccc gtcctctgga aaaacaagtc tgggctgggg caattgacac
   118501 cgttggcgac aaagtgctgg caaaagtgct ggcgcaaatg aattacggcg gctgcgtggc
   118561 ggcctgtggt ctggcgggtg gttttactct gccaaccacg gtcatgccat ttattctgcg
   118621 taatgtccgt ttacaggggg tggattcagt gatgactcca ccagaacgcc gcgcacaagc
   118681 ctggcagcga ctggtcgccg atttaccgga atcattctat acccaggcgg caaaagagat
   118741 atctctggca gaggcaccga aatttgccgg ggccatcatt aataaccaga tccagggtcg
   118801 cacgctggtg aaggttaact aaccatttag cagggaataa taagagaggg aactcatttt
   118861 gaaatcatta ttcaatcggt taacgggaaa agcggttagc cggacagctt tcgtcgaaca
   118921 tcttggtcat gaagttgtac aacatcatcc aaactggaaa gtcatgattt cgactgacca
   118981 taaattgatg cgcactgtta ctccactaaa cagccattat tgatacgcct ccgtcgcctg
   119041 ttaggtttat ggtgctttgc ctgggcgacg ctacgcttaa cctgttactg atttctggta
   119101 ccatggggtg aataatctga ttttatttgc ctacaaatta accactcgaa cgtatttgat
   119161 gctgagcatt gtcaggcggt tgattttgcg tgctttagca ttcacgttta tccagacgat
   119221 gcagtgaaaa ttgggcaatc cccagcaacc gctgcgtaat gtcgtctatc ttgtcgcgat
   119281 cctggcatcc ctacattatt tgtggtctgt gaagattatc tcattgcagc cctcatcttc
   119341 gcagggctgg ctttacagct tttagcctta cgttataaga agttccgtcg atgatggcgc
   119401 taatttcgtg aattgtgcgg cttgttgcaa attacacggt gttgaaggtt atttacatgt
   119461 tagctgttga ttatcttccc tgataagacc agtatttagc tgccaattgc tacgaaatcg
   119521 ttataatgtg cgacctcgtc ctccctgacg cagtttttgc gctgcggaaa aggtgacatt
   119581 ggcgcaacga aggtatattt tgttttttgc cggaggatag cagcagatcg ctgcacaatg
   119641 tccgtcaagt ctaacattga cactctgggg caaaatagac cggcgtcccg gcctgctgga
   119701 atttatcgct atgcatacag ctgtcggggc atacgcttta cagacggcgg tgaaacgcct
   119761 gtcacaatca cactaaacaa agagtacgga acccactcat ggatattcgt aagattaaaa
   119821 aactgatcga gctggttgaa gaatcaggca tctccgaact ggaaatttct gaaggcgaag
   119881 agtcagtacg cattagccgt gcagctcctg ccgcaagttt ccctgtgatg caacaagctt
   119941 acgctgcacc aatgatgcag cagccagctc aatctaacgc agccgctccg gcgaccgttc
   120001 cttccatgga agcgccagca gcagcggaaa tcagtggtca catcgtacgt tccccgatgg
   120061 ttggtacttt ctaccgcacc ccaagcccgg acgcgaaagc gttcatcgaa gtgggtcaga
   120121 aagtcaacgt gggcgatacc ctgtgcatcg ttgaagccat gaaaatgatg aaccagatcg
   120181 aagcggacaa atccggtacc gtgaaagcaa ttctggtcga aagtggacaa ccggtagaat
   120241 ttgacgagcc gctggtcgtc atcgagtaac gaggcgaaca tgctggataa aattgttatt
   120301 gccaaccgcg gcgagattgc attgcgtatt cttcgtgcct gtaaagaatt gggcatcaag
   120361 actgtcgctg tgcactccag cgcggatcgc gatttaaaac acgtattact ggcagatgaa
   120421 acggtctgta tcggccctgc tccgtcagta aaaagttatc tgaacatccc ggcaatcatc
   120481 agcgccgctg aaatcaccgg cgcagtagca atccatccgg gttacggctt cctctccgag
   120541 aacgccaact ttgccgagca ggttgagcgc tccggcttta tcttcatcgg cccgaaagca
   120601 gaaaccattc gcctgatggg cgacaaagta tccgcaatcg cggcgatgaa aaaagcgggc
   120661 gttccttgcg taccgggttc tgacggcccg ctgggcgacg atatggataa aaaccgcgct
   120721 attgctaaac gcattggtta tccggtgatt atcaaagcct ccggcggcgg cggtggtcgc
   120781 ggtatgcgcg tagtgcgcgg cgacgctgaa ctggcacaat ccatctccat gacccgtgcg
   120841 gaagcgaaag ctgctttcag caacgatatg gtttacatgg agaaatacct ggaaaatcct
   120901 cgccacgtcg agattcaggt actggctgac ggtcagggca acgctatcta tctggcagaa
   120961 cgtgactgct ccatgcagcg ccgccaccag aaagtggtcg aagaagcacc ggcaccgggc
   121021 attaccccgg aactgcgtcg ctacatcggc gaacgttgtg ctaaagcgtg tgttgatatc
   121081 ggctatcgcg gcgcaggtac tttcgagttc ctgttcgaaa acggcgagtt ctatttcatc
   121141 gaaatgaaca cccgtattca ggtagaacat ccggttaccg aaatgatcac cggcgttgac
   121201 ctgatcaaag aacagctgcg tatcgctgcc ggtcaaccgc tgtcgatcaa gcaagaagaa
   121261 gttcacgttc gcggccatgc ggtggaatgc cgtatcaacg ccgaagatcc gaacaccttc
   121321 ctgccaagtc cgggcaaaat cacccgtttc cacgcaccag gcggttttgg cgtgcgttgg
   121381 gagtctcata tctacgcggg ctacaccgta ccgccgtact atgactcaat gatcggtaag
   121441 ctgatttgct acggtgaaaa ccgtgacgtg gcgattgccc gcatgaagaa tgcgctgcag
   121501 gagctgatca tcgacggtat caaaaccaac gttgatctgc agatccgcat catgaatgac
   121561 gagaacttcc agcatggtgg cactaatatc cactatctgg agaaaaaact cggtcttcag
   121621 gaaaaataag actgcaaaag cgtcaaaagg ccggattttc cggccttttt tattactggg
   121681 gatcgacaac ccccataagg tacaatcccc gctttcttca cccatcaggg acaaaaaatg
   121741 gacactcgtt ttgttcaggc ccataaagag gcgcgctggg cgctggggct gacccttttg
   121801 tatctggcgg tatggttagt agccgcttac ttacctggcg ttgcccccgg ttttaccggc
   121861 tttccgcgct ggtttgagat ggcctgcatc ctgacgccgc tgctgtttat tggactgtgc
   121921 tgggcgatgg tgaaatttat ctatcgcgat atcccactgg aggatgacga tgcagcttga
   121981 agtgattcta ccgctggtcg cctatctggt ggtggtgttc ggtatctctg tttatgcgat
   122041 gcgtaaacgg agcacaggca ccttccttaa tgagtatttc ctcggcagcc gctctatggg
   122101 cggtattgtg ctggcgatga cgctcaccgc gacctatatc agtgccagtt cgtttatcgg
   122161 cgggcctggc gctgcttata aatacggcct gggttgggta ttgctggcga tgattcagct
   122221 tcctgcggtc tggctttcgc tcggtattct cggcaagaag ttcgcgattc ttgcgcgccg
   122281 ctacaatgcg gtgacgctga acgatatgct atttgcccgc taccagagtc gtctgctggt
   122341 gtggctggcg agtttaagtt tgctggttgc gttcgttggt gcgatgaccg tgcagtttat
   122401 cggcggtgcg cgtctgctgg aaaccgcggc gggtattcct tatgaaacag gtttgctgat
   122461 ttttggcatc agcattgcgc tctacaccgc cttcggcggc tttcgcgcca gcgtgctgaa
   122521 cgacaccatg caagggctgg tgatgctgat tggcaccatt gtgctgctta ttggcgtggt
   122581 ccatgctgct ggcggcttaa gcaatgcggt agagacgctg caaaccatcg atccgcaact
   122641 ggttacgcca caaggcgctg acgatattct gtcgcctgcg tttatgacgt cgttctgggt
   122701 gctggtgtgt tttggcgtga ttggcctgcc acatactgcg gtgcgctgta tctcttataa
   122761 agacagcaaa gccgtacatc gggggatcat catcggtacg attgtggtcg caatcctgat
   122821 gttcggtatg cacctggccg gagcgttagg tcgggcggtg atccccgatc tcaccgtacc
   122881 ggacctggtg atcccgacgt taatggtaaa agtgttgcca ccgtttgctg ccgggatctt
   122941 cctggctgca cctatggctg cgatcatgtc gacgatcaat gcccagttgc tgcaaagttc
   123001 cgctacgatc attaaagatc tctatttgaa tatccgtccg gatcaaatgc aaaacgagac
   123061 gcgtctgaag cggatgtcgg cggtaattac gttagttctc ggtgcgttgc tgctgcttgc
   123121 cgcctggaag ccgccagaaa tgatcatctg gctgaatttg ctggccttcg gtgggctgga
   123181 agccgttttc ctgtggccgc tggtactggg tctttactgg gaacgcgcca acgccaaagg
   123241 cgcgctaagt gcgatgattg ttggcggcgt actgtatgcc gtactcgcga cgctgaatat
   123301 tcagtacctg ggcttccacc ctatcgtgcc ctcgttacta ctaagtttgc tggctttcct
   123361 ggtcggaaac cgtttcggta catccgtccc gcaagctacc gttttgacta ctgataaata
   123421 aagagttttg ccatgccttg gatccaactg aaactgaaca ctaccggcgc gaacgcggaa
   123481 gatcttagcg atgcgctgat ggaagcgggt gccgtttcta tcacttttca ggatacccac
   123541 gatacgcctg tatttgagcc gctgccgggc gaaacgcgcc tgtggggcga caccgatgtg
   123601 attggtctgt tcgacgctga aaccgatatg aacgacgtgg tggcgattct ggaaaaccat
   123661 ccgctgctcg gcgcgggctt cgcgcataaa atcgaacaac tggaagataa agactgggag
   123721 cgcgaatgga tggataattt ccacccgatg cgctttggtg aacgactgtg gatctgccca
   123781 agctggcgtg atgtgccgga cgaaaacgcc gtcaacgtga tgttagaccc agggctggcg
   123841 tttggtacgg gtacccatcc aactacctct ctgtgcctgc aatggctcga cagcctcgat
   123901 ttaaccggta aaacagtcat cgactttggc tgcggttccg gcattctggc tatcgcggcg
   123961 ctgaaactgg gtgcagcaaa agccattggt attgatattg atccgcaggc gattcaggcc
   124021 agccgcgaca acgcggaacg taatggcgtc tctgaccgtc tggaactcta cttaccgaaa
   124081 gatcagccag aagaaatgaa agccgacgtg gtggtcgcta acatccttgc aggcccatta
   124141 cgtgaactgg caccgttaat cagcgtcctg ccagtttcag gcggtttgct gggcctttcc
   124201 ggtattctgg caagccaggc agagagcgtt tgcgaagctt atgccgacag cttcgctctg
   124261 gacccggtcg tggaaaaaga agagtggtgc cgcattaccg gtcgtaagaa ttaaccttct
   124321 catcgccgta gggtgacgca gggcgaagtg cgagcaaggt cacaaaaggc acgtgaattt
   124381 gccgattatt tacgcaaatt tgcgtgccaa aattttcatt cataaagaaa aagtgagaac
   124441 ttactcaaat ttctttgagt gtaaatttta gtcactattt t
//
LOCUS       DACBVI010000002        77635 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.002, whole genome shotgun sequence.
ACCESSION   DACBVI010000002 DACBVI010000000
VERSION     DACBVI010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 77635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.002"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..593)
                     /locus_tag="IU325_00620"
     CDS             complement(<1..593)
                     /locus_tag="IU325_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP5807536.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(664..2778)
                     /gene="fusA"
                     /locus_tag="IU325_00625"
     CDS             complement(664..2778)
                     /gene="fusA"
                     /locus_tag="IU325_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP5807537.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2875..3345)
                     /gene="rpsG"
                     /locus_tag="IU325_00630"
     CDS             complement(2875..3345)
                     /gene="rpsG"
                     /locus_tag="IU325_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP5807538.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3442..3816)
                     /gene="rpsL"
                     /locus_tag="IU325_00635"
     CDS             complement(3442..3816)
                     /gene="rpsL"
                     /locus_tag="IU325_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003852912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP5807539.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3942..4229)
                     /gene="tusB"
                     /locus_tag="IU325_00640"
     CDS             complement(3942..4229)
                     /gene="tusB"
                     /locus_tag="IU325_00640"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HAP5807540.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIILIDYTDFVRLTVKHSSQMAW"
     gene            complement(4237..4596)
                     /gene="tusC"
                     /locus_tag="IU325_00645"
     CDS             complement(4237..4596)
                     /gene="tusC"
                     /locus_tag="IU325_00645"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HAP5807541.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPLTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4596..4982)
                     /gene="tusD"
                     /locus_tag="IU325_00650"
     CDS             complement(4596..4982)
                     /gene="tusD"
                     /locus_tag="IU325_00650"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HAP5807542.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHELSCVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQSGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4982..5704)
                     /locus_tag="IU325_00655"
     CDS             complement(4982..5704)
                     /locus_tag="IU325_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5807543.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5871..6683)
                     /gene="fkpA"
                     /locus_tag="IU325_00660"
     CDS             complement(5871..6683)
                     /gene="fkpA"
                     /locus_tag="IU325_00660"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003827768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAP5807544.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6904..7122
                     /locus_tag="IU325_00665"
     CDS             6904..7122
                     /locus_tag="IU325_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HAP5807545.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7172..7762)
                     /locus_tag="IU325_00670"
     CDS             complement(7172..7762)
                     /locus_tag="IU325_00670"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP5807546.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7857..8057)
                     /locus_tag="IU325_00675"
     CDS             complement(7857..8057)
                     /locus_tag="IU325_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HAP5807547.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8067..9872)
                     /gene="kefB"
                     /locus_tag="IU325_00680"
     CDS             complement(8067..9872)
                     /gene="kefB"
                     /locus_tag="IU325_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HAP5807548.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGAADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVLSVIVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHTDT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9872..10423)
                     /gene="kefG"
                     /locus_tag="IU325_00685"
     CDS             complement(9872..10423)
                     /gene="kefG"
                     /locus_tag="IU325_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HAP5807549.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10554..12473
                     /locus_tag="IU325_00690"
     CDS             10554..12473
                     /locus_tag="IU325_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP5807550.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDHKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKEKENANSAQARKDQKRREAELRAQTQPLRKEI
                     ARLEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLE
                     AQEQLEQMLLEGQSN"
     gene            12473..13495
                     /locus_tag="IU325_00695"
     CDS             12473..13495
                     /locus_tag="IU325_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP5807551.1"
                     /translation="MAQITTTNANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PHWQRLELPDGDFVDLAWSEDPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQRESGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPIDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13489..13707
                     /locus_tag="IU325_00700"
     CDS             13489..13707
                     /locus_tag="IU325_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HAP5807552.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13761..14630
                     /locus_tag="IU325_00705"
     CDS             13761..14630
                     /locus_tag="IU325_00705"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HAP5807553.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14685..15089)
                     /locus_tag="IU325_00710"
     CDS             complement(14685..15089)
                     /locus_tag="IU325_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HAP5807554.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15391..16023
                     /gene="crp"
                     /locus_tag="IU325_00715"
     CDS             15391..16023
                     /gene="crp"
                     /locus_tag="IU325_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HAP5807555.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16074..18164
                     /locus_tag="IU325_00720"
     CDS             16074..18164
                     /locus_tag="IU325_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5807556.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18227..19450)
                     /gene="argD/dapC"
                     /locus_tag="IU325_00725"
     CDS             complement(18227..19450)
                     /gene="argD/dapC"
                     /locus_tag="IU325_00725"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HAP5807557.1"
                     /translation="MATEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGQARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MHRFAHAVAKVIGGK"
     gene            complement(19536..20099)
                     /gene="pabA"
                     /locus_tag="IU325_00730"
     CDS             complement(19536..20099)
                     /gene="pabA"
                     /locus_tag="IU325_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HAP5807558.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGVGVFKGLANPLTVTRYHSLVVEPDSLPVCFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(20131..20733)
                     /locus_tag="IU325_00735"
     CDS             complement(20131..20733)
                     /locus_tag="IU325_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HAP5807559.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKEKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20723..20890)
                     /locus_tag="IU325_00740"
     CDS             complement(20723..20890)
                     /locus_tag="IU325_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2559 family protein"
                     /protein_id="HAP5807560.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20995..21567)
                     /gene="ppiA"
                     /locus_tag="IU325_00745"
                     /gene_synonym="rotA"
     CDS             complement(20995..21567)
                     /gene="ppiA"
                     /locus_tag="IU325_00745"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HAP5807561.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21838..23019
                     /gene="tsgA"
                     /locus_tag="IU325_00750"
     CDS             21838..23019
                     /gene="tsgA"
                     /locus_tag="IU325_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HAP5807562.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSIAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23281..25824
                     /gene="nirB"
                     /locus_tag="IU325_00755"
     CDS             23281..25824
                     /gene="nirB"
                     /locus_tag="IU325_00755"
                     /EC_number="1.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HAP5807563.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25821..26147
                     /gene="nirD"
                     /locus_tag="IU325_00760"
     CDS             25821..26147
                     /gene="nirD"
                     /locus_tag="IU325_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HAP5807564.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26273..27079
                     /gene="nirC"
                     /locus_tag="IU325_00765"
     CDS             26273..27079
                     /gene="nirC"
                     /locus_tag="IU325_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HAP5807565.1"
                     /translation="MFTDTITKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            27098..28471
                     /gene="cobA"
                     /locus_tag="IU325_00770"
     CDS             27098..28471
                     /gene="cobA"
                     /locus_tag="IU325_00770"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAP5807566.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLSQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28718..28885
                     /locus_tag="IU325_00775"
     CDS             28718..28885
                     /locus_tag="IU325_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4223 domain-containing protein"
                     /protein_id="HAP5807567.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            complement(28992..30077)
                     /locus_tag="IU325_00780"
     CDS             complement(28992..30077)
                     /locus_tag="IU325_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP5807568.1"
                     /translation="MKTYPLQSLTLIEAQQKQFALVDTICRHFPGSEFLTRGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIAQMGLTLITADFNDLLALKQVVDEQQPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKLGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(30089..31393)
                     /locus_tag="IU325_00785"
     CDS             complement(30089..31393)
                     /locus_tag="IU325_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5807569.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWFGLFVPA"
     gene            complement(31405..31758)
                     /locus_tag="IU325_00790"
     CDS             complement(31405..31758)
                     /locus_tag="IU325_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 family protein"
                     /protein_id="HAP5807570.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(31769..32647)
                     /locus_tag="IU325_00795"
     CDS             complement(31769..32647)
                     /locus_tag="IU325_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HAP5807571.1"
                     /translation="MSIDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     AQGVRNVIEMTNRYMGRNAQFMLDVMHETGINVVACTGYYQDAFFPQHVATRSVQELA
                     QEMVDEIDQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DERRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            complement(32644..33870)
                     /locus_tag="IU325_00800"
     CDS             complement(32644..33870)
                     /locus_tag="IU325_00800"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP5807572.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSAIWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITVNLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLIEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(33870..35033)
                     /locus_tag="IU325_00805"
     CDS             complement(33870..35033)
                     /locus_tag="IU325_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HAP5807573.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAALPVAHQ
                     GHLVQIPCHQVVDAVAQGTDVITVFTLDKAREVSAAAVKAGRVQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLLSGIHRGEPEIVGRYDSLGNSLGA"
     gene            complement(35117..35479)
                     /locus_tag="IU325_00810"
     CDS             complement(35117..35479)
                     /locus_tag="IU325_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP5807574.1"
                     /translation="METRLNLLCDAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDAELLAELAQSSHWQAVVQLHQVLLKEFTLEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(35496..36401)
                     /locus_tag="IU325_00815"
     CDS             complement(35496..36401)
                     /locus_tag="IU325_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5807575.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRADDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(36562..37566)
                     /gene="trpS"
                     /locus_tag="IU325_00820"
     CDS             complement(36562..37566)
                     /gene="trpS"
                     /locus_tag="IU325_00820"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP5807576.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(37559..38317)
                     /locus_tag="IU325_00825"
     CDS             complement(37559..38317)
                     /locus_tag="IU325_00825"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HAP5807577.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLATAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGITPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(38310..38987)
                     /locus_tag="IU325_00830"
     CDS             complement(38310..38987)
                     /locus_tag="IU325_00830"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP5807578.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(39005..39841)
                     /locus_tag="IU325_00835"
     CDS             complement(39005..39841)
                     /locus_tag="IU325_00835"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAP5807579.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(39948..41234)
                     /gene="damX"
                     /locus_tag="IU325_00840"
     CDS             complement(39948..41234)
                     /gene="damX"
                     /locus_tag="IU325_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HAP5807580.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPELEEEIDESEDETVDEERVERRPRKRKKVASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(41326..42414)
                     /gene="aroB"
                     /locus_tag="IU325_00845"
     CDS             complement(41326..42414)
                     /gene="aroB"
                     /locus_tag="IU325_00845"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP5807581.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(42471..42992)
                     /gene="aroK"
                     /locus_tag="IU325_00850"
     CDS             complement(42471..42992)
                     /gene="aroK"
                     /locus_tag="IU325_00850"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HAP5807582.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(43394..44632)
                     /gene="hofQ"
                     /locus_tag="IU325_00855"
     CDS             complement(43394..44632)
                     /gene="hofQ"
                     /locus_tag="IU325_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HAP5807583.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSVAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(44544..44948)
                     /gene="hofP"
                     /locus_tag="IU325_00860"
     CDS             complement(44544..44948)
                     /gene="hofP"
                     /locus_tag="IU325_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HAP5807584.1"
                     /translation="MRGKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGANCEPPHWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(44938..45378)
                     /gene="hofO"
                     /locus_tag="IU325_00865"
     CDS             complement(44938..45378)
                     /gene="hofO"
                     /locus_tag="IU325_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HAP5807585.1"
                     /translation="MDMLFDWWFATSPRLRQLCWAFWLMMLVTLIFLSSTHQEESDAL
                     IRLRASHHQQWAALYRLVDATPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(45362..45901)
                     /locus_tag="IU325_00870"
     CDS             complement(45362..45901)
                     /locus_tag="IU325_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HAP5807586.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     NAEARVDTVLLQAEQQLARSLQITKPRLLERQQLREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNSLETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDGHAL"
     gene            complement(45901..46680)
                     /gene="hofM"
                     /locus_tag="IU325_00875"
     CDS             complement(45901..46680)
                     /gene="hofM"
                     /locus_tag="IU325_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HAP5807587.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQRLANTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGITSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKVY"
     gene            46800..49352
                     /gene="mrcA"
                     /locus_tag="IU325_00880"
     CDS             46800..49352
                     /gene="mrcA"
                     /locus_tag="IU325_00880"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HAP5807588.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(49520..50080)
                     /gene="nudE"
                     /locus_tag="IU325_00885"
     CDS             complement(49520..50080)
                     /gene="nudE"
                     /locus_tag="IU325_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HAP5807589.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRL"
     gene            50400..52535
                     /gene="igaA"
                     /locus_tag="IU325_00890"
     CDS             50400..52535
                     /gene="igaA"
                     /locus_tag="IU325_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HAP5807590.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEEHSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            52600..53268
                     /locus_tag="IU325_00895"
     CDS             52600..53268
                     /locus_tag="IU325_00895"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HAP5807591.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            53279..53680
                     /gene="hslR"
                     /locus_tag="IU325_00900"
     CDS             53279..53680
                     /gene="hslR"
                     /locus_tag="IU325_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HAP5807592.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            53705..54583
                     /locus_tag="IU325_00905"
     CDS             53705..54583
                     /locus_tag="IU325_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP5807593.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(54717..56441)
                     /locus_tag="IU325_00910"
     CDS             complement(54717..56441)
                     /locus_tag="IU325_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HAP5807594.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNLSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQIDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYGYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKSSL
                     PWTGPLRCLIKTALLAAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVSLYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQQQISEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            complement(56447..56596)
                     /locus_tag="IU325_00915"
     CDS             complement(56447..56596)
                     /locus_tag="IU325_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5807595.1"
                     /translation="MSGIAFLNEIDANSIGKVSIHHANFFDYFLKKAKYYGPDAIINP
                     LFDSG"
     gene            56819..58441
                     /gene="pckA"
                     /locus_tag="IU325_00920"
     CDS             56819..58441
                     /gene="pckA"
                     /locus_tag="IU325_00920"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP5807596.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKSLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIENIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            58558..58875
                     /locus_tag="IU325_00925"
     CDS             58558..58875
                     /locus_tag="IU325_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003847996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP5807597.1"
                     /translation="MFTFIELQGFSKRRQLLLPDDEFRAFQELLIQDPTAGDIIAGTG
                     SFRKIRWNRSGMGKRGGIRIIYYNVTRNGRIYLALIYPKNEQDDLTEEQRKALKLLSE
                     KLL"
     gene            58934..59230
                     /locus_tag="IU325_00930"
     CDS             58934..59230
                     /locus_tag="IU325_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP5807598.1"
                     /translation="MKDELFADLLASAEEMVRIEKGEEAPEPEHVHTFSEIDVKAIRE
                     ATGLRQQDFAVAVGVSYDLVKSWETKRRQPTGAPRKLLLLLQANPFIINQLKTI"
     gene            complement(59262..60614)
                     /gene="envZ"
                     /locus_tag="IU325_00935"
     CDS             complement(59262..60614)
                     /gene="envZ"
                     /locus_tag="IU325_00935"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HAP5807599.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFVSLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGATKEG
                     "
     gene            complement(60611..61330)
                     /gene="ompR"
                     /locus_tag="IU325_00940"
     CDS             complement(60611..61330)
                     /gene="ompR"
                     /locus_tag="IU325_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HAP5807600.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            61522..62034
                     /gene="greB"
                     /locus_tag="IU325_00945"
     CDS             61522..62034
                     /gene="greB"
                     /locus_tag="IU325_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HAP5807601.1"
                     /translation="MRIIKQTKGINEMKTPLVTREGYEKLKQELNYLWREERPEVTKK
                     VTWAASLGDRSENADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAW
                     VEIENDDGVTHRFRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASW
                     YVNAIEYVKP"
     gene            62131..64452
                     /locus_tag="IU325_00950"
     CDS             62131..64452
                     /locus_tag="IU325_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP5807602.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDELANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            64892..65119
                     /gene="feoA"
                     /locus_tag="IU325_00955"
     CDS             64892..65119
                     /gene="feoA"
                     /locus_tag="IU325_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HAP5807603.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            65136..67457
                     /gene="feoB"
                     /locus_tag="IU325_00960"
     CDS             65136..67457
                     /gene="feoB"
                     /locus_tag="IU325_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HAP5807604.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVNIELLATRKSVSSCCAASTTGDCH"
     gene            67457..67693
                     /gene="feoC"
                     /locus_tag="IU325_00965"
     CDS             67457..67693
                     /gene="feoC"
                     /locus_tag="IU325_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HAP5807605.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQALNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKVCLREWWALR"
     gene            67896..68789
                     /gene="rpnA"
                     /locus_tag="IU325_00970"
     CDS             67896..68789
                     /gene="rpnA"
                     /locus_tag="IU325_00970"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HAP5807606.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPNLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGFQKGIRTNALNIAKTMLDAGVS
                     LEDVLRFTSLTVEDLAEINHQ"
     gene            complement(69009..69779)
                     /gene="bioH"
                     /locus_tag="IU325_00975"
     CDS             complement(69009..69779)
                     /gene="bioH"
                     /locus_tag="IU325_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HAP5807607.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGAMSLADMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQSLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPAEFCRMLVALKQRV"
     gene            69817..70500
                     /gene="gntX"
                     /locus_tag="IU325_00980"
     CDS             69817..70500
                     /gene="gntX"
                     /locus_tag="IU325_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HAP5807608.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARSDKTLCPQCGLPA
                     THAHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALARLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRLLHCRWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            70559..71134
                     /gene="nfuA"
                     /locus_tag="IU325_00985"
     CDS             70559..71134
                     /gene="nfuA"
                     /locus_tag="IU325_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HAP5807609.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            71495..72811
                     /gene="gntT"
                     /locus_tag="IU325_00990"
     CDS             71495..72811
                     /gene="gntT"
                     /locus_tag="IU325_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAP5807610.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(72859..74940)
                     /gene="malQ"
                     /locus_tag="IU325_00995"
     CDS             complement(72859..74940)
                     /gene="malQ"
                     /locus_tag="IU325_00995"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HAP5807611.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLEDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWKLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFADCWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRSPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLEAMFADDGVNKLLKD
                     LDRRRRAAAKK"
     gene            complement(74950..77343)
                     /gene="malP"
                     /locus_tag="IU325_01000"
     CDS             complement(74950..77343)
                     /gene="malP"
                     /locus_tag="IU325_01000"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HAP5807612.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRGNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVENTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
BASE COUNT        18684 a        21115 c        19527 g        18309 t
ORIGIN      
        1 gaatccagga agccagccag ttccaggatt ttcgcttccc actctgcgtc gccttccagc
       61 gctttcagag cagaaccacg aacgatcgga gtgtcgtcgc ccgggaaatc gtactgagac
      121 agaagttcac gaacttccat ttcaaccagt tccagcagct cttcgtcatc aaccatgtcg
      181 catttgttca ggaacacgat gatgtacgga acgcctacct gacgacccag caggatgtgc
      241 tcacgagtct gcggcatcgg gccgtcagtc gcagcaacta ccaggatcgc gccgtccatc
      301 tgagcagcac cggtgatcat gtttttaaca tagtcggcgt gccccgggca gtctacgtgt
      361 gcgtagtgac gggtcggggt gtcatattca acgtgagaag tgttgatggt gataccacga
      421 gctttttctt ccggagcgtt atcgatctgg tcgaatgcac gagcagcacc gccgtaggtt
      481 ttagccagta cggtggtgat tgcagcggtc agagtagttt taccgtggtc aacgtggccg
      541 atagtaccaa cgttaacgtg cggttttgta cgttcaaatt tttctttaga cacggctata
      601 ttccttacta tagtgctctc cccttcagga gagagcacgg gactttggta ttaaccctta
      661 ggcttattta ccacgggctt caattacggc ctgagcaacg ttactcggcg cttcatcata
      721 cttcaggaat tccatggtgt atgatgcacg acctttggtc agagaacgca gctgagttgc
      781 atatccgaac atttcagaca gcggtacttc agcgtggatc ttaacgccag taacttcaga
      841 ttcctgacct ttgagcatac cacgacgacg gctcaagtca ccgataacgt caccggtgtt
      901 ctcttccgga gtttctactt caaccttcat gatcggctca agcagaactg gtttcgcttt
      961 cttaaagcct tctttaaagg cgatagaagc agccagttta aacgccagtt cagaggagtc
     1021 aacgtcatgg taagaaccga agtgcagacg aatacccatg tctactaccg ggtagcctgc
     1081 cagcggacct gctttcagct gttcctggat acctttatca acggccggga tgtattcgcc
     1141 agggattaca ccacctttaa tgtcgttgat gaactcgtag cctttcgggt ttgaacccgg
     1201 ctccagcggg tacatgtcga taacaacatg accatactga ccacgaccac cagactgttt
     1261 cgcgtgttta ccttcaacat cggtaacttt ctggcggata gtttcacggt aagcaacctg
     1321 cggtttacct acgttcgctt caacgttgaa ttcacgcttc atacggtcaa cgatgatgtc
     1381 gaggtgcagt tcgcccatac ccgcgatgat ggtctggtta gattcttcgt cagtccatac
     1441 acggaaagac gggtcttctt tagccagacg gcccagagcc agacccattt tttcctggtc
     1501 agctttggtt ttcggttcaa ctgcgatgga gattaccggc tcagggaatt ccatacgttc
     1561 cagaatgatc ggcgcatccg ggtcacacag ggtgtcacca gtggttacgt ctttcagacc
     1621 gatagcagca gcgatgtcgc ccgcgcgaac ttctttgatc tcttcacgtt tgttagcgtg
     1681 catctgaacg atacgaccga aacgctcacg tgcagctttc acggagttca gtacggtatc
     1741 accagagtta accacaccgg agtaaacacg gaagaaggtc aggttaccaa caaacgggtc
     1801 ggtagcgatt ttgaacgcca gtgcagagaa cggctcgtca tcacttgcgt gacgttcagc
     1861 cggagtatct ttaccgtcgt ccaggatacc gttgatcgca ggtacgtcaa ccggggatgg
     1921 caggtaatca attaccgcat ccagcatcgc ctgaacacct ttgttcttga acgcagaacc
     1981 acaggttacc aggatgattt cgttgttcag aacgcgctga cgcagagcac ctttgatttc
     2041 tgcttcagtc agttcttcac cacccaggta tttttccatc agctcttcag aagcttcagc
     2101 tgcggattcg atcaggttct ggtgccattc gttagccagt tcaaccatgt ctgccgggat
     2161 atcttcgtat tcgaaggtta cgccctggtc agcgtcgttc cagttgatag ctttcatttt
     2221 caccaggtca acaacaccgg tgaaatgttc ttcagcacca atcgccagct gcagcggaac
     2281 cgggttcgcg cccagacggg ttttgatctg gttaacaact ttcaggaagt tcgcacccat
     2341 gcggtccatt ttgttaacga acgcaatgcg cggaacttta tatttgtttg cctgacgcca
     2401 tacggtttca gactgcggct gaacaccacc aactgcgcag taaaccatta ccgcaccatc
     2461 gagaacacgc atggaacgtt ctacttcgat tgtgaagtca acgtgccccg gggtgtcgat
     2521 gatgttgatg cgatgcggct catactgctt agccatacca gaccagaatg cagtagtcgc
     2581 agcggaagtg atggtaatac cacgttcctg ctcctgctcc atccagtcca tggttgcagc
     2641 gccgtcgtga acttcaccga ttttatggtt tacaccggtg tagaacagaa tacgttcggt
     2701 agtagtggtt ttaccggcgt cgatgtgcgc actgataccg atgttacggt agcgtgcgat
     2761 gggtgttgta cgagccattt gtttcctcgt ttatctttta ggcgttcaat ttaagtagcc
     2821 caaagcgggc tgcttactgg aagcgcccgc ctggtgacta aaactccgaa gggattacca
     2881 acggtagtgt gcgaacgcct tgttggcttc ggccatacgg tgaacgtctt cacgtttctt
     2941 aactgcagta cctttgtttt ctgcagcatc agaaagttcg ttcgccaggc gcagagccat
     3001 ggatttatca ccgcgtttac gagcagcttc aacgatccaa cgcattgcca gagcattacg
     3061 acgaaccgga cggacttcaa ctggtacctg ataagtagaa ccaccaacgc ggcgagactt
     3121 aacttctaca gtcgggcgca cgttttcgag agctacttcg aatgcttcca gttcagattt
     3181 accagagcgc tgagccaggg tctccagcgc gctgtatacg atagattcag cagtagattt
     3241 tttaccatct accatcagga tatttacaaa tttagccagc agttctgatc cgaacttcgg
     3301 atccggcaga attttacgct gaccaatgac gcgacgacgt ggcatggaaa tactccgttg
     3361 ttaattcagg attgtccaaa actctacgag tttagtttga catttaagtt aaaacgtttg
     3421 gccttactta acggagaacc attaagcctt aggacgcttc acgccatact tggaacgagc
     3481 ctgcttacgg tctttaacgc cggagcagtc aagcgcacca cgtacggtgt ggtaacgaac
     3541 acccgggagg tctttaacac gaccgccacg gatcaggatc acggagtgct cctgcaggtt
     3601 gtgaccttca ccaccgatgt aggaagtcac ttcgaaaccg ttagtcagac gaacacggca
     3661 tactttacgc agcgcggagt tcggtttttt aggagtggta gtatatacac gagtacatac
     3721 gccacgtttt tgcgggcatg cttccagcgc aggcacgttg cttttcgcaa ctttgcgagc
     3781 acgtggtttg cgtaccagct ggttaactgt tgccattaaa tagctcctgg ttttagcttt
     3841 tgcttcgtaa acacgtaata aaacgtcctc acacaatatg aggacgccga attttagggc
     3901 gatgccgaaa aggtgtcaag aaatatacaa cgatcccgcc atcaccaggc catctggctg
     3961 gagtgcttaa ccgtaagtct gacgaaatca gtatagtcaa tgagaatgat atcgttcgaa
     4021 atttgaccag tcaaaccgcg ggcaataagg tcttcgttca gggcatagac cttaatgggg
     4081 gcattacgca gactttcaag gtagcggtta ccgtcaactg cggcagttac accatcttgc
     4141 aataacagca gttcgtcgcc ttcactgagc agacgcagca gcgcagcaaa atccgtcagc
     4201 cagggtgaac gatgtaatgt gtgcagcata aacgcctcaa aatctcaaaa taacatcgta
     4261 gttggcgagt tcgcggcgta aggcatctgc ttcgagcggc gtggcttcga caacaaaggg
     4321 tgtcagcgga tctaacccgc gttcgcgcag tgaagccgca caaacccagc actgttcaat
     4381 gtcgtacaga cccaacaatt taaaagtggc aatgtaatca cgcgccagca ctgcatcggg
     4441 cttttgtcct ggcagcagct gaaaaacacc atcagcaata aagaagacgg ccagatcgtc
     4501 agttaatgcg gaagttgcca gtaaagcatc taaaccttcc cgaccagcag ctgtaccatg
     4561 aggtgcagta gaaaaaacaa acgcaattcg tttcatcaga actgtaccac cctgtcacag
     4621 gtcagcgagg cttccgccag cgcgccaagt ccgcttaagg taaatcccga ctgaagattt
     4681 gacgaagcca gccccagcct tcctgcttca gtttcatcga cgataccgcg gcgtaacgct
     4741 gccgctacgc agatattcag cgccacacca tgttgcgcat tcaactgttg ccagccgcgc
     4801 acaaggtcaa attcatcgct tgccggtgag gtcaattggt tagcgttata gaccccttcc
     4861 cgatagaaaa agacgcagct taactcatgg ccttcggcta tcagtgcttg cgcaaactga
     4921 aaagcactac tcgcctgttg cgtaccgtat gctggcccgg tcaccacgat ggcaaaacgc
     4981 attacttatc ttgcccctgg aaatcaccgc tcttgaactg gcggatgtag agatagacag
     5041 tgtgtttgga gatgttcagg cggtcagcaa cctggttgat cgcgtcttta atatcgaaga
     5101 tccctttctc gtagagattt agcacgatct gacgattttt ggcgttatta gaaacattgc
     5161 gatcggcatt cacttcttcg atggtgaact ccagcgtttg ggtaaccaga tcttcaacag
     5221 aagaggcaaa gttgacgctt gaaccgacat ccggggtttc tggcggcaca aaggtgctca
     5281 taatctgcga gaagggaaca tcaagattca tattgatgca cagcagacca attacacgct
     5341 gttcgcggtt acgaatcgcg atagtcaggg acttcattaa tacgccgctt ttggcgcgag
     5401 taaagtagca tttagaaacg ctgctatccg ctcccgtcat atcgtgcagc atacgtagcg
     5461 ccaggtcagt aattggcgaa ccaatcttcc ggcctgtatg ttcaccgtta gcaatgcgaa
     5521 tggctgaaca ttttagatcc tgcaaagagt gcaaaacgat ttcacagtgg gagccaataa
     5581 gcatcgctaa cccgtccacc accgcttcgt aggatttcag aatatcaaaa tcggtctggt
     5641 cgaaaggacg ttgatccagt aaatccaact cactggtttc gttggttaaa agcgacctgg
     5701 acatgaaaaa aaacactcct tttcaggagc ctgtcgttaa cttttcaggg caggctcatt
     5761 aatgatgcgg gtaactaaat taatacagcg gaggttccgc tttccagcac taattatatc
     5821 cggcctgtaa taaaaaaacc gccgcctggt caggcggcgg ttcttaatgc ttatttttta
     5881 gcagaatctg cggctttcgc atcagcttcc ggctttgcat cagccttcgg cgctggtttc
     5941 acatccagca gctctacgtc aaacaccagg gtagaattcg gtgggatccc cggaacaccc
     6001 gctttgccgt aagccagttc tggtggaata accagtttga tcttaccgcc tttcttgatg
     6061 ttcttcagac cttctgtcca acccggaata acaccgtcca gacggaaaga gagcggttca
     6121 ccacgggtgt aagagttgtc gaactcttta ccgtcgatca gcgtaccttt gtagttcacg
     6181 acaacagtat cgctgtcttt cggtgcttcg cctttaccgg cttcgactac ctgataaacc
     6241 agaccagttg aagaggtttt cacacctttc tctttggcaa atttctcgcg gtactcttta
     6301 cctttcgctt cgttatctgc cgcgtctttt tccatcttcg cctgagcaga agacttcacg
     6361 cgagcttcga atgcttgcag tgtctgttcg atctcttggt cggagagttt gctcttatca
     6421 gcaaatgcat cctgaacacc agcgatcagc tgatctttat ccagtttgat gcccagtttt
     6481 tcttgttctt taagagagtt ttccatgtaa cgacccagcg aggcacccag tgcataagct
     6541 gatttctgat cgtcattttt gaacgctgct ttgctgtcag cagttgtagc aggttttgca
     6601 gcttcagcag caaaagtgat tggtgcatgc agggcaacgg ccattgtggt cgccagcagc
     6661 gttactttaa acagtgattt catccatatc tccaggatcg gggcatctca ccccagggtt
     6721 aactattatc agaagggtac tataaagcgt tgtcgaacaa atctacatac agacacgccc
     6781 tattatcatc tattttcaga ctctttttgt ttaaattagt ttcgatgacc gcgaaatgag
     6841 tgctgtctcg ggcagcaaag ttaagtagaa tccgcggcga ccattcgaca aaagaggtga
     6901 atcatgcagg atttatcatt ggaagcacgc ctggcagagc tggagagccg actggctttt
     6961 caggaaatca ccattgaaga actgaacgtc acggtgaccg ctcatgaaat ggagatggcg
     7021 aaactgcgcg atcatctgcg tctgctgacc gagaagctaa aagccagcca gccgtcgaac
     7081 atcgcgtcgc aggctgaaga aacgccaccg ccacattatt gaggcgtaaa aaaagcgggg
     7141 attcccccgc ttttttgtca ctttttcggt attagtggca accgcaaccg ccgttacctt
     7201 taccgccgca gcagccttcg ccaccgtgtt catgaccgtg atcgtggcca tgaccaccgc
     7261 agcaaccgtc gtggtcgtga tcgtggtgat gatcgtgcgc accgtgaacg tggccatgag
     7321 ccagttcttc ttcagtcgct tcgcgaatcg ccacaacttc aacgttgaat ttcaggttct
     7381 gaccggccag catgtggtta ccatcaacca cgacgtgatc gtcttcaacc gcagtgattt
     7441 caaccggtac cggaccctgg tcggtttcag ccaggaaacg catacctacc tgcagttcat
     7501 caacgcccat aaatacgtct ttaggaacgc gttgcaccag gttttcgtcg tactgaccgt
     7561 aagcgtcgtt cgcgccaaca gcgacatcaa atttgtcgcc aacttcgtga ccttccagcg
     7621 ccgtttccag gccagagatc agggaaccgt gaccatgcag gtagtccagc ggcgcactca
     7681 ccggagactc atcaaccaac acaccgtctt ctgtacgtac ctgataggcc aggctgacca
     7741 ccaggtcttt tgctactttc atgatatctc ctgagcatgg gaagaatagt ggcgcagatt
     7801 gtagcggaat tctgcagccg tgtactcact agcttaaaaa aactcggcgc atatcgctag
     7861 tccggatgaa aaatcccgat cacttgctca tctttgcgaa cgtgatcgcg ggcttctttg
     7921 tccgcttctc gcatctgatg tccgcactta acacattcaa caatatcaat attattttcg
     7981 cgccacatcg ccattgaatc ctgcgcctga caggccgggc attttgcgcc cgcaataaaa
     8041 cgttttcgga ttgccatctt taccctctac tcaaattcat cccagccgtc cagctggcgt
     8101 cgttcttgtt gcatttcacg ctggaaaatc tcttccagtt cgcgtcgggc ttccctggcg
     8161 cgagaaattt gtacggtatc ggtatgcatt gggatgagct ctcgcagcat ccgcatatcc
     8221 aggcgacgaa aatgcagttg cgcccgctgt gcctgatgcg gatgcatgcc aagcgtgacc
     8281 agcgtcttgc gccccagctc taacgcactg gagaatgttt cacgggaaaa ctgcgtcacc
     8341 cctgcctgta ataactcatg cgcttccaca cgtccgcgcg ctcgcgcaag aatatgcaaa
     8401 tgcggaaagt gctgctggca tatttccacc agcttcatgg tgtcttccgg ctcgttacag
     8461 gtaatgacga tagactcagc ggcctctgct cccgcagaac gtaaaagatc gacctgcgtg
     8521 gcgtcgccgt aataaacttt gtagccatat ttacgcatca ggttaacggc actgatatcc
     8581 cgctccagca cagtaatgcg cattttattt gccatcagca aacgaccaat cacctgacca
     8641 aaacgcccaa agcccacgac aatgacctgg ggtttatcat cgttgaccca cggtttttcg
     8701 tcttcttctt ccggtccgtt aaactggcgg gatagccatt tatccaccag cttcatcagc
     8761 agcggcgtgg tcatcatgga aagcgtcacc gtcaccagca acagcgccat ctggtcaccc
     8821 tggaataagc gttgtgaaga agcggtagaa aagagcacaa aggcaaactc accgccctga
     8881 ctcaacacgc cagcaaactg catccgctct gagctacgca cgccatacag tcgcgccagc
     8941 agatacagca cgagaatttt caccgccacc aacacaataa cgcttaacac cacccacaac
     9001 agatgggtat aaagcacacc gaggttgagc gacattccga ccgagataaa aaatagcccg
     9061 agcagcaacc ccttaaacgg gtcgatagct gtttccagtt cgtggcgata ttcgctttct
     9121 gccagcaaca cgcccgcaat aaacgtaccg agcgccatcg acagccccag cgcatccata
     9181 aacaatgcgg aacccaacac cagcagcagc gtcgcggcgg tgaacacttc ccgcacacca
     9241 gaagctgcaa taaaacggaa taccggacgc agtaaataac gcccgccaat cagcatgccg
     9301 acaaacgcca gcaccttcat gccgattttc atccagtcga aatgttcgtc tgccgccccc
     9361 gccaacaacg gcactaacgc cagtgccggg atcaccgcca gatcctgaaa cagcagtacc
     9421 gagaaaccta actggccgga ttcgctgcga ttcatccctt tctcacgcat cagttgcaac
     9481 gccattgcgg ttgaggacat cgcaaggcca ataccaccga ccaccgccgc ctgccaggcg
     9541 aaatccgtca gcatcaataa tcccgccagc aacgccgcgc ttaacagcac ctgtgccgca
     9601 cctacgccaa aaatcgaacg ccgcaattgc caaagcttgg agggattcaa ctccaggccg
     9661 atgataaaca tcaggaacac cacgccgagt tccgaaaagt ggaggatctc atcgacgtcg
     9721 ctaataaaac ccagccccca cgggccaatt gcaatccctg ccagcaaata tcccaacaca
     9781 gcgccaatac ccagccgcga tgccagcggc accgcagcca ccgccgcgaa gagaaacaac
     9841 actcctgcga gtaaaaaatc ggaaccttcc atcagcggcc tcctggagac agcggatttg
     9901 ccagccagtc accgtaggct ctggcgtgac tcgccagctc ctgtgcgctt tgccgtctcg
     9961 cccagtaaat aatgatggga ctcaaccaat gcatccggca catgcccgcc gctagttcaa
    10021 aggggcgcag aacatcgctc atcgggtagc gattcaaggc gtcataacgg taagcacttt
    10081 ccggctcgcc ggtggtaatc acgctacgcc agtactttcc cgccagttgg tttccccccg
    10141 gcccgctggc aaaaccacga cttaataccc ggtccagcca ctctttcagt agcgccgggc
    10201 agctataggt ataaagagga tgctgaaaga caatcacctc gtgctcgcgc agtaatgcct
    10261 gctcacgggg gatatcaata aaaaaatcgg gatagtgcgc gtaaaggtcg tgcacggtaa
    10321 cattgctgag ctgcgtggcc ggtttaagca gtacccggtt tgccaccgag tcctgagatt
    10381 ccggatgggc atacagcagc aaaactttcg ctggctgaga catcatcccc ctcccggtat
    10441 ggtttttgtg tatagtcgct gttttgggct accattgcgc ccggtgcggc agctcgccca
    10501 tacattacat tatcataatg ataagttaac atagtctgaa catacggcac cttatgattg
    10561 ttttctcctc gttacaaatt cgtcgcggcg tgcgcgtcct gctggataat gccaccgcca
    10621 ccatcaaccc cgggcagaaa gtcggcctgg tgggtaaaaa cggctgtggt aaatctaccc
    10681 tgctggcatt gctgaaaaat gaaatcagcg ccgacggcgg cagctacacc tttccgggaa
    10741 gctggcaact ggcgtgggtg aatcaggaaa cgccggcgtt accgcaagcg gcgctggaat
    10801 atgtcattga cggcgaccgt gaatatcgtc aactggaagc gcagctacac gacgccaacg
    10861 aacgtaacga cgggcacgcc atcgccacta ttcatggcaa gctggatgct atcgacgcat
    10921 ggagtattcg ctcccgtgcc gccagcctgc tgcacggcct cggtttcagc aatgaacaac
    10981 tggagcgccc ggtaagtgat ttctctggtg gctggcgtat gcgtcttaac cttgcccagg
    11041 cgctgatttg ccgttcagac ttgctgctgc tcgacgaacc gactaaccac ctcgatctcg
    11101 atgccgttat ctggctggaa aaatggctga agagctatca gggcacgctg atcctgatct
    11161 ctcacgaccg cgacttcctc gatccgatcg ttgataaaat tattcatatc gaacaacaaa
    11221 gcatgttcga gtacaccggc aactacagtt cgtttgaagt gcagcgcgcc acccgtctgg
    11281 cgcagcaaca agcgatgtac gaaagccagc aggaacgcgt ggcgcatctg caaagttata
    11341 tcgaccgttt ccgtgccaaa gccaccaaag cgaagcaggc ccagagccgc attaagatgc
    11401 ttgagcgtat ggagctgatt gcccccgcgc acgtcgacaa cccgttccgc tttagcttcc
    11461 gcgcgccgga aagcctgcca aatccgttac tgaagatgga aaaggtcagc gcaggctatg
    11521 gcgatcgcat tattctcgac tcgattaaac tgaatctggt ccccggctcg cgcattggtc
    11581 tgctaggccg caacggcgcg ggtaaatcga cattaatcaa actgttagcc ggtgaacttg
    11641 cgccagtcag cggtgaaatt ggtctggcga aagggatcaa gctcggctac ttcgcccagc
    11701 atcaacttga atacctgcgc gccgacgaat cgcctattca acatctggca cgtttagcgc
    11761 cgcaggaact ggagcaaaaa ctgcgtgact acctcggcgg ctttggtttc cagggcgata
    11821 aagtgacgga agaaacgcgc cgcttctccg gtggggaaaa agcccgcctg gtgctggcat
    11881 taattgtctg gcagcgtccg aatctgctgc tgctcgacga accgactaac caccttgacc
    11941 tcgacatgcg tcaggcactg actgaagcat taatcgagtt cgaaggcgcg ctggttgtcg
    12001 tctcgcacga ccgtcatttg ctgcgttcca ccactgacga tctctacctg gttcacgatc
    12061 ataaagtcga accgttcgac ggcgatctgg aagattatca acagtggttg agcgacgtac
    12121 aaaagcagga aaaccagacc gacgaagcgc caaaagagaa agagaacgcg aacagcgccc
    12181 aggcacgtaa agatcagaaa cgtcgggaag cggagctgcg tgcgcaaacc cagccactgc
    12241 gtaaagagat tgcccgtctg gaaaaagaga tggagaagct gaacgcacag ctggcgcagg
    12301 cggaagagaa actcggcgac agcgaactgt acgatcaaag ccgtaaagcg gagttgaccg
    12361 cctgcctgca acagcaagcc agcgccaaat ccggcctgga agagtgcgaa atggcgtggc
    12421 tggaagccca agagcagctt gagcagatgc tgctggaagg ccaaagcaac tgatggcgca
    12481 gataacgacg accaatgcca atgaattcag cagcagtgct gaattcaccc ctatgcgcgg
    12541 ctttagcaat tgtcatctac aaaccatgct gccgcgtctg tttcgtcgca aggtgaaatt
    12601 caccccgcac tggcagcgac tggagttgcc cgacggcgat tttgtcgatc tcgcgtggag
    12661 tgaagatcct gcacaggcgc ggcataaacc gcgtttagtc gtgtttcacg ggctcgaagg
    12721 cagtctcaat agcccttacg cccacggtct ggttgaggcg gcgcaaaagc gtggctggct
    12781 gggcgtggtg atgcattttc gcggatgcag cggtgaacca aaccgtatgc accgcattta
    12841 ccattcgggc gaaaccgaag acgccagttg gtttttacgc tggctgcaac gcgagtctgg
    12901 tcatgcgcca acggctgccg tcggctattc gctcggcggt aatatgctgg cctgtttgct
    12961 ggcaaaagaa ggcaatgatc tcccgattga tgcggcggtg attgtctctg cgccgtttat
    13021 gctggaagcc tgtagctatc atatggaaaa gggcttttcc cgcgtctatc agcgttactt
    13081 gctgaacctg ttaaaagcca atgccgcacg caagctggct gcctaccccg gaacgctgcc
    13141 gattaatctc gcgcagttaa aatcggtacg tcgcatccgt gaatttgacg atctaatcac
    13201 cgccagaatt cacggctacg ctgacgctat cgactattat cgtcagtgta gcgccatgcc
    13261 gatgctcaat cggatagcca aaccgacgct gattattcac gccaaagacg atccgtttat
    13321 ggatcatcag gtgatcccga aaccggaaag tctccccccg caggtggagt atcagctgac
    13381 tgaacatggc ggtcatgttg gctttattgg cggtacatta cttcatccgc aaatgtggct
    13441 ggagtcacgc attcctgact ggttaacaac gtatctggag gcgaaatcat gttgattccg
    13501 tggcaagacc tctcccccga aacgctggaa aatttaattg aaagctttgt gttacgtgaa
    13561 ggcaccgatt atggtgaaca tgagcgtaca cttgaacaga aagtcgccga cgtcaaacgc
    13621 cagctacagt gcggagaagc ggtgctggta tggtcggagc tgcacgaaac ggtcaatatc
    13681 atgccgcgca gtcagtttcg cgaataacct gcaaccgtcg gcggacgaga taataatgat
    13741 agctaagtca tggagttact atgtctgcca aacatccggt cattgcggta acaggatcca
    13801 gcggcgcggg gaccaccacc accagcctcg cgtttcgtaa aatattcgcg cagttaaatc
    13861 tgcatgcagc tgaggtggaa ggcgacagtt ttcaccgtta cacccgcccg gaaatggaca
    13921 tggcgatccg caaagcgcgc gacgccgggc ggcatatcag ctacttcggc cccgaagcga
    13981 acgactttgg cctgctggaa caaaccttca ttgaatatgg tcagagcggc aaagggaaat
    14041 ctcgcaaata tctgcatacc tacgatgaag ccgtaccgtg gaatcaggta ccggggacat
    14101 tcaccccctg gcaaccttta ccggaaccca cggatgtact gttttatgaa ggtttacacg
    14161 gcggcgtagt cacgccacag cataacgttg cgcagcatgt ggatttactg gttggcgtgg
    14221 tgcctatcgt taaccttgag tggattcaaa aactgatccg cgacaccagc gagcgcgggc
    14281 actcacgaga agcagtgatg gactcggtag tgcgttcaat ggaagactat atcaactaca
    14341 tcacaccgca gttttcccgc acccatctta acttccagcg cgttcccacc gtcgacacct
    14401 caaacccgtt cgcggcaaaa ggtatcccgt cgctggatga aagctttgtg gtgatccatt
    14461 ttcgtaatct ggaagggatc gattttccct ggctgctggc gatgttgcaa ggctcattca
    14521 tttcccacat caatacttta gtggtgcctg gcggcaaaat gggtctggca atggaattaa
    14581 ttatgctgcc gctggtgcaa cgattgatgg aagggaagaa aatcgagtaa ctctgctatt
    14641 acgccggata aaatactatc cggcttcaca acgggatagt taaatcacgc ggcaaccact
    14701 tcatacgagt gagtaatatt caccgctttt tccagcatca acgccactga acaatatttc
    14761 tcggcagaga gatctaccgc acgcgcaacc gctgcgtctt tcaggtcgcg accggtgacg
    14821 ataaaatgca gattaatgtg cgtaaacagg cgtggtgcct cttcgcggcg ttcagaggtc
    14881 aattttactt cacaatcgac cacatcctga cgcccttttt gcagaataga aaccacatcg
    14941 atggcactgc aaccacccgc cgccatcaac accatttcca tcggacttgg tgctttatcg
    15001 cctgagttgc cgtccattaa aatctggtga ccagaggcgg attcgcccag aaaagttaac
    15061 ccttcgaccc acttcactcg cgcttgcatt tttgctactc cactgcgtca attttcctga
    15121 cagagtacgc gtactaacca aatcgcgcaa cggaaggcga cctgggtcat gctgaagcga
    15181 gacaccagga gacacaaagc gaaagctatg ctaaaacagt caggatgcta cagtaataca
    15241 ttgatgtact gcatgtatgc aaaggacgtc acattaccgt gcagtacagt tgatagcccc
    15301 ttcccaggta gcgggaagca tatttcggca atccagagac agcggcgtta tctggctctg
    15361 gagaaagctt ataacagagg ataaccgcgc atggtgcttg gcaaaccgca aacagacccg
    15421 actctcgaat ggttcttgtc tcattgccac attcataagt acccatccaa gagcacgctt
    15481 attcaccagg gtgaaaaagc ggaaacgctg tactacatcg ttaaaggctc tgtggcagtg
    15541 ctgatcaaag acgaagaggg taaagaaatg atcctctcct atctgaatca gggtgatttt
    15601 attggcgaac tgggcctgtt tgaagagggc caggaacgta gcgcatgggt acgtgcgaaa
    15661 accgcctgtg aagtggctga aatttcgtac aaaaaatttc gccaattgat tcaggtaaac
    15721 ccggacattc tgatgcgtct gtctgcacag atggcgcgtc gtctgcaagt cacttcagag
    15781 aaagtgggca acctggcgtt cctcgatgtg acgggccgca ttgcacagac tctgctgaac
    15841 ctggcaaaac aaccggatgc tatgactcac ccggacggta tgcaaatcaa aattacccgt
    15901 caggaaattg gtcagattgt cggctgttct cgtgaaaccg tgggacgcat tctgaagatg
    15961 ctggaagatc agaacctgat ctccgcacac ggtaaaacca tcgtcgttta cggcactcgt
    16021 taatcccgtc ggagtggcgc gttacctggt agcgcgccat tttgtttccc ccgatgtggc
    16081 gcaggctgat ttatcacccc gatatcaact atgcacttcg acaaacgctg gtgctatgtt
    16141 tgcccgtggc cgttgggtta atgcttggcg aattacgatt cggtctgctc ttctccctcg
    16201 ttcctgcttg ttgcaatatt gcgggccttg atacgcctca taaacgtttt ttcaaacgct
    16261 taatcattgg tgcgtcgctg tttgccacct gtagcttgct gacacagcta ctactggcaa
    16321 aagatgttcc tctgcccttt ttgctgaccg gattaacgct ggtacttggc gtcactgctg
    16381 agctggggcc attgcacgca aaattgcttc ccgcatcgct gctcgccgcc atttttaccc
    16441 tcagtctggc gggatacatg ccggtctggg aaccgttgct catctatgcg ttgggcactc
    16501 tctggtacgg attgtttaac tggttttggt tctggatctg gcgcgaacaa ccgctgcgcg
    16561 agtcactaag tctgctgtac cgtgaactgg cagattattg tgaagccaaa tacagcctgc
    16621 ttacccagca caccgaccct gaaaaagcgc tgccgccgct gctggtgcgc cagcaaaaag
    16681 cggtcgatct aattacccag tgctatcagc aaatgcatat gctttccgcg caaaataata
    16741 ccgattacaa gcggatgctg cgtattttcc aggaggcgct ggatttgcag gaacatattt
    16801 cggtcagttt gcatcagccg gaagaggtgc aaaagctggt cgagcgtagc catgcggagg
    16861 aagttattcg ctggaatgcg caaaccgtcg ccgctcgcct gcgcgtgctg gctgatgaca
    16921 ttctttacca tcgcctgccg acgcgtttta cgatggaaaa gcaaattggc gcactggaaa
    16981 aaatcgcccg ccagcatccg gataatccgg ttgggcaatt ctgctactgg catttcagcc
    17041 gcatcgcccg cgtgctgcgc acccaaaaac cgctctatgc gcgtgactta ctggccgata
    17101 aacagcggcg aatgccatta cttccggcgc tgaaaagtta tctgtcacta aagtctccgg
    17161 cgctacgcaa tgccggacga ctcagtgtga tgttaagcgt tgccagcctg atgggcaccg
    17221 cgctgcatct gccgaagtcg tactggatcc tgatgacggt attgctggtg acgcaaaatg
    17281 gctatggcgc aacccgtctg aggattgtga atcgctccgt gggaaccgtg gttgggttaa
    17341 tcattgcggg cgtggcgctg cactttaaaa ttcccgaagg ttacaccctg accttgatgc
    17401 tgattaccac cctcgccagc tacctgatat tgcgcaaaaa ctacggctgg gcgacggtcg
    17461 gttttaccat taccgcagtg tataccctgc aactgttgtg gctgaacggt gagcagtaca
    17521 tccttccacg tcttatcgat accattattg gttgtttaat tgctttcggc ggtactgtct
    17581 ggctgtggcc gcagtggcag agcgggttat tgcgtaagaa cgcccatgac gctttagaag
    17641 cctatcagga agcgattcgc ttgattctga gcgaggaccc gcaacctacg ccactggcct
    17701 ggcagcgaat gcgggtaaat caggcgcata acactctgta taactcattg aatcaggcga
    17761 tgcaggaacc ggcgtttaac agccattatc tggcagatat gaaactgtgg gtaacgcaca
    17821 gccagtttat tgttgagcat attaatgcca tgaccacgct ggcgcgggaa caccgggcat
    17881 tgccacctga actggcgcaa gaatatttac agtcttgtga aatcgccatt cagcgttgtc
    17941 agcagcgact ggagtatgac gaaccgggta gttctggcga tgccaatatc atggatgcgc
    18001 cggagatgca gccgcacgaa ggcgcggcag gtacgctgga gcagcattta cagcgggtta
    18061 ttggtcatct gaacaccatg cacaccattt cgtcgatggc atggcgtcag cgaccgcatc
    18121 acgggatttg gctgagtcgc aagttgcggg attcgaaggc gtaatgcagg ctaaaccatc
    18181 ggatacagcg ttcacgccgc atccgacatc tttggcctga tgcgtcttat ttacccccaa
    18241 tcaccttcgc caccgcgtgg gcgaaacgat gcatcccttc atcgatatcc gcatcttcca
    18301 ccaccagcga cggcgcaaaa cgcatcacat caggcccggc attcagcacc attacgccag
    18361 cctctgcgcc cgcatacagg aaatcacgcg cctgaccttt gtactgtggt ttcagctccg
    18421 cgccaattaa cagccccata ccgcgaatat cgctaaacac atcgtactgc tgatcgatct
    18481 tctgcaaatg gtcaacaaaa cgctggcgtt tcgcctgaat gccttccagc acttcagggg
    18541 tattgatgat atcaaacgcc gcgcccgcta ctgcacaggc cagaggatta ccgccgtagg
    18601 tggaaccgtg agaaccagga tgaaacgcag aagcaatttc cgccgtggtc agcatggcgc
    18661 taatcgggaa gccgccgcct aacgctttcg cagaggtcag aatatccggc gtcacaccgt
    18721 agtgcatgta agcaaacaaa tcgccagtcc gccccatccc gcactgcact tcatcaaaca
    18781 ccaataatgc ctgatgttga tcgcacagct cgcgcaagcc ctgcaaaaac tctggcgtcg
    18841 cagccgtcac gccgccctcg ccctggatcg gctcaaccac caccgcacag gtgtgatcat
    18901 ccatcaccgc tttcactgcg tggagatcgt taaagggaac gtggatgatg tctgccggtt
    18961 tcggcccaaa gccgtcggaa tattttggct gcccacccac cgaaacggta aacagcgagc
    19021 gaccatgaaa agcgttatgg aaggcaataa ttttggtttt gaacgggcta tgacgcacac
    19081 aggcgtaatg gcgtgccagt ttaaaggcgg tttcgttagc ttccgtgccg gagttcataa
    19141 acaccacgcg ttcggcaaac gttgcctcaa tcagtttacg accaagacgc agcgccggtt
    19201 cattggtgaa aacgttactg atatgccaca gagtttcgcc ctgggttttt aacgcgttca
    19261 ccagcgcagg atggcaatgg cccaacgccg taactgcaat gccacccgcg aaatcgacat
    19321 actccttgcc ttgctgatcc cagattcggc tcccctgacc ttttaccgga ataaactctg
    19381 ccggagcata aatcggcagg atcacttcat cgaaagtcgc gcgtgtaatt gctgtttgtt
    19441 cagttgccat ctcatgatca ccctgttaca cataaataaa tgtgaaatta taatcacaaa
    19501 atatgcataa aaaatcactt aatagcaatc agaaatcagc gatgcaggaa attagccagc
    19561 agttgatgtc cttgttcgct gagaatactt tctggatgga actgcacgcc ttccagatcc
    19621 cactggcgat ggcgaatacc cataatctcg cgggtttcgc tccaggccgt cacttcaaag
    19681 cacacaggta atgagtcagg ttccaccacc agcgaatggt agcgtgtcac ggtaagtgga
    19741 tttgccagcc ccttaaatac gcccacacca ttatgtgtaa tcggcgaggt tttgccgtgc
    19801 atgacctttg cggcgcgcac aactttgccg ccaaatgcct gcgccattgc ctggtgaccg
    19861 aggcaaacgc caagaatcgg caagcgcccg gcatagtggc gaataacgtc aagggagatc
    19921 ccggcttcat ctggcgtaca gggtccgggt gagatgacaa ttttttgtgg tttaagggcg
    19981 tcgatatccg ccagcgtcaa cgcatcgttg cgcttaacca gcacatccgc ccccagttca
    20041 caaaagtact ggtagaggtt ccaggtaaaa gaatcgtagt tatctataag caggatcatg
    20101 gcggctccgg tacgaaagaa ccgcgctatt ttactcagat tccccggctt cgcttaccac
    20161 tttgctaaat atcatctgca gtgcggtgag atcccccatt gccccactct gatttgcctg
    20221 attccaggcc tctttttcga taccctgcca gctcagttgg tagcccgcat gaatcgccag
    20281 ttgctcgaag aagatccgct gtgccagtcc gcttcccaca cggaagggat gcagcacgtt
    20341 gatttcacaa tagtaatgtg ccagccgctc gacgaacttc tctttctcca ggccaaccag
    20401 ataaccttct tcctccagat cctgcatcag ggcattgccc tctttttcga tataggcaaa
    20461 gtggcagaac ggcgtatcac cctgataaat atcaacttca cgcaattgcc ccgcccagtc
    20521 gaaaatatcc tgatacagct ggcgatggat agtgcgcaaa tgcggtaaac cgcgcaccag
    20581 cggaccaagc tcaatggtcg cagcacgcag cgccgtcatt tcgtaagcgg cctgttccag
    20641 ccgctgctgc tggcggatgt tcagccggtt acgcatgata tcaaggccag gataaagata
    20701 cggatcgcgc ccttcgccga atttatcgct catagtgcct cctcagctct tcaaggcgcg
    20761 ctaaagcctc tgcggcagta agagtgacta aaggcatctc gacgccttca agacggcgac
    20821 tggcctggaa attacgatta cgctgaagct cccagagacg ggacttttgc ttatcggtga
    20881 gtttcttcac ttgatgcctc cctgaacgtg tccatttgcc acaagtataa gcggcaaatc
    20941 tgggttacgc cgggagagca gaagcgcggg caagattgcc cgcgcgagaa atcattacgg
    21001 caggacttta gcggaaagga taactaccgg ttttgacggc acattctggt acggaccaac
    21061 gtcatgagtc ggcacctggg aaatcttatc ggcaacgtcc atgcctttca ccactttacc
    21121 aaataccgcg taaccgaaat cacgctgacc gtggtcaagg aaggcgttat cggcaacgtt
    21181 gataaagaac tggctggtgg cgctgtcttt gtcagcggta cgcgccatcg cgatggtgcc
    21241 acgcgtgttg cgcaggccgt tatcggcttc atttttgatt ggcgggtttg gttttttctg
    21301 ctgcatctgc tcggtgaaac cgccgccctg aatcataaag ccaggaatga cgcggtgaaa
    21361 ggtagtgttg ttataaaaac cgctgttcac ataatcgaca aagttttgca cagacactgg
    21421 cgctttttgt ttatccagtt ccagttcgat gttaccagct gaggttgtca acaatacgtg
    21481 cgggtcccct ttcgctgcca ttgctgcggg agaaagagca gaaagagcga aaacagcagc
    21541 catcgccgcc agggtcgatt tgaacatgag atttccttaa caaacagagc agaaaaaaag
    21601 caagtgaaga gattctaaat agccttcaag aacaaggcca tccctttacc taattttacg
    21661 tatctgaaat atatgtaacc tgacgatcac tttttattct gtgataaaaa tcacctctta
    21721 aaatgcaatt tagcaaccga ttgcaataaa acatttaatc agatcacaaa atcacctaaa
    21781 atcgcccgct cgcggcatag cccgctatgc cattttattt ttgaacgcag gccaatcatg
    21841 actaacagca atcgcatcaa gctcacatgg attagctttc tctcctacgc actgaccggt
    21901 gcgttggtta ttgtcaccgg gatggtgatg ggaaatatcg ccgattattt caatctgcct
    21961 gtttccagta tgagtaatac cttcaccttc ctcaacgccg gcattttaat ctctatcttc
    22021 ctcaacgcct ggctgatgga aatcgtcccg ttgaaaacgc agttacgttt tggctttctc
    22081 ctgatggtgc tggcggttgc cggtttgatg ttcagccaca gcctggcgct gttctcggcg
    22141 gcgatgttca ttctcggggt ggtcagcggc atcaccatgt cgattggtac attcctgata
    22201 acacaaatgt atgaagggcg tcagcgcggt tcccgcctgt tatttaccga ctccttcttc
    22261 agtatggctg ggatgatttt cccaatgatc gccgcgtttc tactggcgcg cagcattgag
    22321 tggtactggg tttatgcctg catcgggctg gtgtatgtcg ctatttttat tctgaccttc
    22381 ggctgtgagt tcccggcact gggcaaacat gcaccaaaaa cggatgctcc ggtagagaaa
    22441 gaaaagtggg ggatcggcgt gctgtttctc tccattgcgg cactgtgcta catcctcggt
    22501 cagttaggtt ttatctcctg ggtgcctgag tatgccaaag gcctgggcat gagcctgaat
    22561 gatgctggta cgctggtgag taacttctgg atgtcataca tggtcggcat gtgggcgttc
    22621 agctttattc ttcgcttctt tgatttgcaa cgtatcctga ccgtactggc gggtctggcc
    22681 gcgattctga tgtacgtctt taacaccgga acaccggcac atatggcgtg gtcaattctc
    22741 gccctgggct tcttctccag cgcgatctat accaccatca tcaccctggg ttcacagcag
    22801 accaaagtac cgtcgccaaa actggttaac tttgtcctga cctgcgggac catcggtact
    22861 atgttgacct ttgtggttac cggcccgatt gttgaacata gcggtccgca ggcggcactg
    22921 cttacagcaa acggtctgta cgctgtcgtc tttgtgatgt gcttcctgtt aggtttcgtc
    22981 agccgtcacc gtcagcataa caccctgacc tctcattaat tgctcatgcc gggcggcgct
    23041 atcgtcgtcc ggccttttcc tctcttaccc tgctacgtgc atctatttct ataaacccgt
    23101 tcaatttgtc tgttttttgc acaaacatga aatgtcagac aattccgtga cttaagaaaa
    23161 tttatacaaa tcagcaatat accccttaag gagtatataa aggtgaattt gatttacatc
    23221 aataagcggg gttgctgaat cgttaaggta ggcggtaata gaaaagaaat cgaggcaaaa
    23281 atgagcaaag tcagactcgc aattatcggt aacggtatgg tcggccatcg ctttatcgaa
    23341 gatcttcttg ataaatctga tgcggccaac tttgatatta ccgttttctg tgaagaaccg
    23401 cgcatcgctt atgaccgcgt acacctctcg tcttacttct ctcaccacac cgccgaagag
    23461 ctgtcgctgg tgcgcgaagg cttctacgag aaacacggca tcaaagttct ggtcggcgaa
    23521 cgcgctatca ccatcaaccg tcaggagaag gtgattcact ccagcgccgg acgtaccgtt
    23581 ttttatgaca agctgatcat ggcgaccggt tcctacccgt ggatcccacc aatcaaaggt
    23641 tctgatactc aggactgctt tgtctatcgc accattgaag acctcaacgc tatagaatcc
    23701 tgcgcccggc gcagcaagcg tggtgccgtt gtaggtggcg gcctgttagg tctggaagcc
    23761 gctggcgcgc tgaaaaactt aggcattgaa acccacgtta tcgaatttgc ccctatgctg
    23821 atggcagaac agcttgatca gatgggcggc gagcagctgc gtcgcaaaat cgaaagtatg
    23881 ggcgtgcgcg ttcacaccag caaaaacacc cttgagattg tgcaggaagg tgttgaagcg
    23941 cgtaaaacca tgcgttttgc cgacggcagc gaactggaag tcgactttat cgtcttctct
    24001 accggtatcc gtccgcgcga taagctggca acccagtgtg gtctggacgt tgctccgcgt
    24061 gggggtatcg tcataaatga ttcctgccag acttccgatc cggatatcta cgccatcggt
    24121 gaatgcgcaa gctggaacaa ccgtgtattt ggtctggtag caccaggcta caaaatggcg
    24181 caggtcgccg ttgaccatat tcttggcagc gaaaacgcct ttgaaggtgc tgaccttagc
    24241 gccaagctga aactgctggg cgtagatgta ggtggtattg gtgatgcgca cggtcgcacg
    24301 cctggcgcac gtagctacgt ttacctcgac gaaagcaaag agatctacaa acgcctgatt
    24361 gtcagcgaag acaacaaaac cctgctcggt gcggtactgg tgggcgatac cagcgactac
    24421 ggcaacctgc tgcaactggt gctgaacgct atcgagctgc cggaaaaccc ggattctctg
    24481 attctgccgg cgcactctgg tagcggtaag ccgtctatcg gtgttgataa actgccggac
    24541 agcgcgcaaa tctgctcctg cttcgacgtc accaaaggcg acctgattgc tgctatcaac
    24601 aaaggctgcc acaccgttgc ggcgctgaaa gctgaaacca aagcgggtac tggctgcggt
    24661 ggctgtatcc cgctggtcac tcaggtactg aacgcggaac tggcgaaaca gggcatcgaa
    24721 gttaacaaca acctgtgcga acactttgct tattcgcgtc aggaactgtt ccatttaatc
    24781 cgcgttgaag gcattaaaac cttcgaagaa ctgctggcga aacacggcaa aggctacggt
    24841 tgtgaagttt gtaaaccgac cgtcggttcg ctgctggcct cctgctggaa cgaatacatt
    24901 ctgaagccgg aacatactcc gctgcaggat tcaaacgaca acttcctcgc taacatccag
    24961 aaagacggca cctactcggt gatcccgcgt tctccgggcg gtgaaatcac cccggaaggg
    25021 ctgatggcag taggtcgtat cgcgcgtgaa tttaatctct acaccaaaat caccggctcc
    25081 cagcgcctgg cgatgtttgg cgcacagaaa gacgatctgc cggagatctg gcgtcagctg
    25141 attgaagccg gcttcgaaac cggtcatgcc tatgcgaaag cactgcgtat ggcgaaaacc
    25201 tgcgtgggta gcacctggtg ccgctacggc gttggcgata gcgtcggcct cggcgtggaa
    25261 ctggaaaacc gctacaaagg catccgtacg ccgcacaaaa tgaagttcgg tgtctccggc
    25321 tgtacccgtg aatgttcaga agctcagggt aaagacgtgg gtattatcgc caccgaaaaa
    25381 ggctggaacc tgtacgtttg cggcaacggc ggcatgaaac cgcgtcatgc ggatctgctg
    25441 gcggcggata tcgatcgcga aacgctgatc aaatatctcg accgcttcat gatgttctac
    25501 atccgtactg ccgacaaact gacgcgtacc gcgccgtggt tagaaaacct cgaaggcggc
    25561 atcgattacc tgaaagcggt gatcattgac gacaaactgg ggctgaacgc tcatctggaa
    25621 gaagagatgg cgcgcctgcg tgaagcggta gtgtgtgagt ggactgaaac ggtcaataca
    25681 ccgtccgcgc agactcgctt caaacacttc atcaacagcg acaagcgtga cccgaacgtg
    25741 cagatggtgc cagagcgcga acagcaccgt ccggcaacgc cgtatgaacg tatcccggta
    25801 actctggtgg aggacaacgc atgagccagt ggaaagacat ctgcaaaatc gatgacatcc
    25861 tgcctgaaac cggcgtctgc gcgctgttag gtgacgagca ggtagcaatt ttccgcccgt
    25921 atcacagcga ccaggtgttt gcgatcagca acatcgaccc gttcttcgag tccagcgtgt
    25981 tgtcacgcgg gctgattgcg gaacaccagg gcgagctgtg ggtcgccagc ccgctgaaaa
    26041 aacagcgttt tcgcttaagc gacggcttgt gcatggaaga cgaacagttt tccgtcaagc
    26101 attacgaagc acgagtgaaa gacggcgtgg tacagctgcg cggttaatat tttaacggga
    26161 ggcgcaatgc ctcccctttt tgcatggtcc tgtaataacc ttcggtatat tgcaggacat
    26221 tttttaaact ttttgtttta ttttttgttt ttatttttta aaggataatc aaatgtttac
    26281 agacactatt actaagtgtg cggctaacgc tgcgcgcatt gcacgcctgt cggcaaataa
    26341 cccgctcggc ttttgggtca gctccgcgat ggcgggcgcg tatgtgggtc ttgggatcat
    26401 cctgattttc acgctcggta atttgctcga cccgtccgta cgtcctctgg tgatgggcgc
    26461 gacctttggt atcgccttaa cgctggtgat tatcgccggt tctgaactgt tcaccggaca
    26521 caccatgttc ctcacctttg gagtgaaagc gggcactatc agccacgggc aaatgtgggc
    26581 aatcctgccg caaacctggc tgggtaacct ggttggttcc gtctttgttg ccatgctcta
    26641 tagctggggc ggcggtagcc tgctgccggt agataccagc atcgttcact ccgtcgcgct
    26701 ggctaaaacc actgcaccgg caatggtact gttcttcaaa ggtgcattgt gtaactggct
    26761 ggtttgcctg gctatctgga tggcgctgcg cactgaaggg gcggcgaaat ttatcgctat
    26821 ctggtggtgt ctgctggcat ttatcgcgtc cggctacgag cactccatcg ctaacatgac
    26881 gctgttcgcc ctctcctggt ttggcaacca cagcgaagcc tacacgctgg cgggtattgg
    26941 tcataacctg ctgtgggtga cgctgggtaa tactttatca ggtgccgtat tcatgggatt
    27001 gggttattgg tatgctacgc cgaaagcgaa tcgtccggtt gcggacaaat ttaatcaaac
    27061 tgaaacagct gccggttaat tactaagggg tttttacgtg gatcatttgc ctatattttg
    27121 ccaattacgc gatcgcgact gtctgattgt cggcggtggt gatgtcgcgg aacgcaaagc
    27181 aaggttgctg ttagacgcag gcgctcgctt aacggtgaat gcattagcgt ttattccaca
    27241 gttcaccgca tgggcagatg caggcatgtt aaccctcgtc gaagggccat ttgatgaaag
    27301 ccttctcgac acctgctggc tggcaattgc tgcgacggat gatgacgcgc ttaaccagcg
    27361 cgtcagtgaa gccgctgaag ctcgtcgcat cttctgtaac gtggtcgatg cgccgaaagc
    27421 cgccagcttt attatgccgt caattattga ccgctcaccg ctcatggtag cggtctcctc
    27481 tggcggcacc tctccggttc tggcacgcct gttgcgtgaa aaacttgaat cactgctgcc
    27541 gttgcatctg ggccaggtag cgaaatacgc cgggcaatta cgcggtcgag tgaaacaaca
    27601 gttcgcaaca atgggtgaac gtcgccgttt ctgggaaaaa ctgttcgtta atgaccgtct
    27661 ggcgcagtcg ctggcaaaca acgatcagaa agccattact gaaacgaccg aacaattaat
    27721 caacgaaccg ctcgaccatc gcggtgaagt ggtgctggtt ggcgcaggtc cgggcgatgc
    27781 cgggctgctg acactgaaag gactgcaaca aattcagcag gcagatgtgg tggtctacga
    27841 ccgtctggtt tctgacgata ttatgaatct ggtacgccgc gatgcggatc gcgttttcgt
    27901 cggcaaacgc gcgggatacc actgcgtacc gcaggaagag attaaccaga tcctgctgcg
    27961 ggaagcgcaa aaaggcaaac gcgtggtgcg gctgaaaggc ggcgatccgt ttatttttgg
    28021 ccgtggtggc gaagagctgg aaacactgtg caacgcgggt attccgttct cggtggttcc
    28081 gggtattacc gcagcttctg gttgctctgc ctattcgggt attccgctca cgcatcgcga
    28141 ttatgcccag agcgtacgct taattaccgg acacttaaaa accggtggcg aactggactg
    28201 ggaaaacctg gcggcagaaa aacagacgct ggtgttctat atggggttga atcaggccgc
    28261 gactattcag caaaagctga ttgaacacgg tatgcctggc gaaatgccgg tggcaattgt
    28321 cgaaaacgga acggcagtca cgcagcgcgt gattgacggt acactctcgc agctgggcga
    28381 actggcgcag caaatgaaca gtccatcgct aattattatt ggtcgggttg ttggcctgcg
    28441 cgataaactg aactggttct ccaatcatta atgtaacccg ggccagagaa ttctggcctt
    28501 cttaatgact ctttttatat attcagcaaa taaaacataa ccccttaata aatattatct
    28561 gctaatgggt tatattcacc gttacagcga cttacaaaat taagccattc actcactgac
    28621 gctaattaaa tattcctgta tcgttcttaa tgagcagtct gcttatattc atataaacaa
    28681 tgaatattaa tcaatatata atacataggg aatgtaaatg aacaaattta ttaaagttgc
    28741 actggtaggt gcagtactgg ctacgttaac tgcatgtact ggtcatatcg aaaaccgtga
    28801 taagaactgc tcttacgact acctgctgca cccggcaatt tctatttcta aaatcattgg
    28861 cggttgcggt cctactgcac agtaagtctc tgacagaaat cggctaacac cggtttaatt
    28921 gcctgacgcc ctcggggtta cccgagggcg tatttatttc tgcattcttt tcgaaaacat
    28981 catgcacaag attaaatact ggcaatactc tcacgcaaaa tccgcagcac cgtctcttca
    29041 ccgctgcgat ttgggttaat gcgaatcgcg caatgttcga gttgcggatt cgcctggcga
    29101 aacgttccgg aaagacggta aaagagcggc gggatttcat atttcgactc tgcaccgacc
    29161 gggtaaggca aggcaccgag cttttgcgcc tcttccagta ctctggcggc aatcggctga
    29221 tgaaactcca caatcaacac cttcgactgt gcattggcaa tgacggcgct tttcacttcc
    29281 ggaactgcac caccgttgag caaggccagc aaccgttcag atacccccgc ctgcaccgcg
    29341 tgcatcactg gcgcaaacac cagcccacgc aagacttcca acgcctgagc gccctgaatc
    29401 tggctgccgc cggagtagag cgtggcgcga atacggttaa taacatcagc atcgcctacc
    29461 accgcgccaa caccctctgg cccaaatagc ttgaagcagg aaaatgtcga gacattcgcc
    29521 ccgcattcac agccaatgcg cgccaccttc atcaccgcat agttgtcatc ggttaacgct
    29581 ggaacacctg ccgagcgcaa cgttgccagc acatctgcca gaatgtaccc gtcctgcggc
    29641 tgctggcgcg tatgctgcac cagcgcggca tctggttgtt gctcgtcgac aacctgcttc
    29701 agtgccaaca ggtcattgaa atcagcagtg ataagcgtca gccccatctg cgcaataatg
    29761 acctgagtcg tcgggtaaac aggcgcgtca tgcaccagaa gacgctgccc cggtttgagc
    29821 aaagccgcca acgccgcacg aatcgctcca gtacccgcac cctgtaccag cgccgcagcc
    29881 tgtgcgtgaa atgcatcagc cagcacctgt tccactcgct gggtaatgcg cggttgattc
    29941 agccccggcg ttaagcccaa atccccgcgg gtaagaaact cgctgccggg aaaatggcgg
    30001 caaatcgtgt ccaccagcgc aaactgcttt tgctgcgcct caataagcgt caggctttgc
    30061 aaaggatacg tcttcatagg tgccttcctt aagccggaac gaacaggcca aaccagtaaa
    30121 gaacattcag cagaataccg gtgatcatta ctgccacgac cggtgccgcc attttctgta
    30181 ccggacggcc cagggattcg ttgaggaagt aaatggcaac tgcgatagaa aatccggtat
    30241 aacccgccat tttaattgct gcgaaaatcg aaccgaccag caacgccact tccatcagca
    30301 tattcatggc gttacggatg ttatctgacg cattacgcac cgacggatag cgccccagcc
    30361 atttgccgat cgaacgaagc agcaggactt ccgccgaaat aaccactgcg cctaataccg
    30421 ctgcaaccat cggattcggc gagagatagc ccaccgcata aacaaaggta aagcccgcaa
    30481 ctgcatacac accagtggct aacgcggtgg tggcaatcaa cggtacaaaa cccagcccgc
    30541 gcataaattc cgccagagct gcctggttaa tcagcgtttg tgattgttcc ggcgttacgc
    30601 ctgcggaata tgctttctcc agtgtgaaga tcgacacttc actgccagca aaaatcttca
    30661 tgctggcaac ggcggcaatc agcgccccga caatagcgat atacggtaag tttttgataa
    30721 tccgcgacgt gcgttcttca aacaccgaaa gcccgctggc atcaatatca ttttcatcac
    30781 gatgacgcag gtcgtgagtt atcgcgatcc ccagcagcat caccatgcca ataaagattt
    30841 cgatggattc cggattgaga tgcgggaaat agcgcactac caccacacgg gtcatcagta
    30901 ccaccacggc ggcgatcaga ctttgcttcc agccaaactg gtagaaaatc gccaccaacg
    30961 ggaacagtgc aaaagctgaa accaccggtg agcttaattc ccccaggcta cctaatacat
    31021 ccaccggtag cgcggtcagc agctggttta ccggcaacag gcaagtaagg atcaacacgc
    31081 cccagatagc gccaagacca aacgccatca ggctgtttat cgccagtacg ccgaggatat
    31141 cggtaggaag aaagagtaac catgcgttga gcagcccggt tttcagggta aacgagatcc
    31201 ccaccgaagc cacaaaacca atgctcagac caaaagcgat actccccgcc tcgcgacggt
    31261 tcatatagcc ttcaatcagt tgcggcagga tcgggcggat accgtcatga aaaacggccg
    31321 ccgagcgatg cgccagaagc gatgtcatac ccgtcaggca cgccaccacg ataatctgaa
    31381 tatacagatc catagtcgtg ccttttattt cagatggtta atcagcatcg gaatcgcgtg
    31441 ttcgacgtgc tcaacggaaa gcccaaacgc cactttccct tcggcgatca ttttggcgat
    31501 atgttcgtct ttcgctttaa tgcccggctt ggcaatggtg caacttttgt tatagccaat
    31561 caccgcgatg gcaatcgaca atgccgcgcc cgcaccggta ttacacgcgc cgatgtagta
    31621 atccagttgc ccggatttga ctttcattgc cgcttccatg tcgttgtgaa tgaaaacttc
    31681 aaaacagcca ggagccgtcg cttcaatggt ttttttaatc tgctcacgct gtaagcctgc
    31741 aacaccaatc tttttcatga gtctgtcctt attggaaaaa ttgagaggga gtttcacgta
    31801 acatcacatc cacatcggcc tgactgaatc ctgactggcg caattgcgga ataaaggtgg
    31861 tcaataaata gtcatagcca taaccaccgt tggcttttaa atgggagcgg cgcgtaatat
    31921 ccatcgacag catgacgcgg ttcagcaaac cacggtcacg tagcgcgtgg agcatcgcaa
    31981 tgcgcctttc gtccgggtag taactgttct tgccgatggt gtcgaactgc acatacgcgc
    32041 cgagatcgat catcttcaaa atgttgtcga ggttgtcttt cagatcgcag tgaccaacgg
    32101 tgacgcgcga aagatcaact ccgtgggctt gcaacagagc cagttgttcc agccccatcg
    32161 tgctgaacga cgtatgcgtg gagatcgggc gtccggtctg gttatgtgcc agcgcggcgg
    32221 caataaacac cttctcttcc agcggcgtaa tcttcccttc gctggagccg atctccgcga
    32281 tgatcccggc tttcagttcc gtgccatcga taccctgatc aatttcatcg accatctcct
    32341 gcgccagttc ctgtacgctg cgggtcgcca catgttgcgg gaaaaacgca tcctggtaat
    32401 aaccggtaca ggccaccacg ttgatgccag tttcgtgcat cacatcgagc ataaattgcg
    32461 catttcgccc catgtaacgg ttggtcatct caatcacatt acgcacgccc tgggccatca
    32521 ggtcgttcat ttcctggcaa atgaacgcat actgatcaag gcggcagtcc acgttgtttt
    32581 taaagccgga gagatcaata tgcagatgct catgggcgag ggtgtaaccc gtcggatcaa
    32641 tactcatagg gtgtctcctg caaaccggag ggaggaaaga aaagagcgac cattttgtgg
    32701 cggtggcgca cggaaaaatt cacacacggt agcccccaca tcagaaagcg tggtgcgcac
    32761 accgagctgc gtagcgacca tcccttgctg ataaaccagc actggcacca cttcgcgggt
    32821 atggtggctg tgaccaatgg tcggatcgtt accgtgatcc gccatcacga ccaggcaatc
    32881 atctggctgc atcgcctcaa taagccgggc aaggttacgg tcaacgacct gcaaacgttc
    32941 ggcataacgt gcgacgtctt ccgcgtgacc agcgaggtcg gtttcctgga tattggtgca
    33001 aataaacgcc gtcggatgtg tgttaaattc gtcgagggtg atatccataa tccgctggct
    33061 atccaccagg ttttgccagc tcacgccata aggattgctg acgatatccg ccaccttacc
    33121 caccagcacg gttggcacgc ccgcttcata cagtttttgc ggcacctgca ctttttcatc
    33181 aacaccgtag cccatatgca cgacctggaa gccgttgtca taagcgcctg aacgcggcgc
    33241 attaatgcca ataaagcgcc cttctttact ttctgcggca tcgagaatgc gttgactgtc
    33301 agttaacagg ccgccaaatg taatgacccg accgacctgt acctgctcac gcacgatacg
    33361 accaattttg attgcgtcgt caaaagagat cacagagaga ttgacggtga tgttatagac
    33421 ctggcctaaa tccgcctcga gattatcgcc aatcgcaacc gcctgattga cccacagaaa
    33481 ttgcagatca tcgccacggc gctccacctg ccaaccggcg gatactaatg cctgctcaac
    33541 acggtcaatc acatcgcgaa agggcatcct cagcggcggt aacggacgcg tgcctaaaat
    33601 ttcctgatgc cccataaagg tatcgccacc ttcatgttgc agctctgcca caccccagat
    33661 tgcggagtct gacggctgca tatcacctgg cgcataaccc aatgcgttga ttagccccag
    33721 cttctccagc gttggtagct gcaaatgcgg cagctggctc aggatgtgac cacaagtatt
    33781 cgccccggca tcctgtggac gcaccagcgt gacatctttc attgcgccta cgccaaagct
    33841 atcaatcact aacaccacaa atcgcgccat tacgccccca gagagtttcc aagactgtca
    33901 taacgaccga cgatttctgg ttcgccacgg tgaataccgg acagcaacac cacatcgcta
    33961 cgggtgacaa aaatctgcgt gcgaaaacag agcactactg cactgcttac cggaaactcg
    34021 cctgccagcg gcagggtgta gtcgatactg ctgtcatcca cagttttgag attggtttca
    34081 gtaatctttt gattttctgg cgtaaacacc agcgcatgtt gcgcatgacc acgacggtag
    34141 taaccgccgc cgtagcagta gctgtcgcca cgaaaatgat gggagatttc acttaaccag
    34201 agcatcgcga tacgttcagg ctgatcgccc tgctggtttg ccggaatagt gcccgtcagc
    34261 gcatgaccag gttcggcatg agtcacaccg tattccgcca gcaatggcag cgaagtgcag
    34321 ctggtcgcag aaggtgcgtt cagttgctca atcgcaatac cagatttcgc cagttgatcc
    34381 cgtgcctgta tcagcgtgtg aagattcggt gtcggcaaaa ctttcccggc agcctcatcc
    34441 caaagcaggc aagggaaatg ggttagtccg gctaaatgca accccggcag tttcttaatt
    34501 tcagcaacca cctcgtgcag cgaatgctgt acaaaaccgc tctcctgtcc cggataaaga
    34561 aaatcatcgt cgctataaac tttgagcagc acagactgca cacgtccggc cttcaccgct
    34621 gccgcagaaa cttcccgcgc tttgtcgaga gtaaacacgg tgatgacgtc ggtgccctgt
    34681 gcaacggcgt caaccacctg atgacaaggg atttgtacta aatgcccctg atgcgccacc
    34741 ggcaaagcag cgcggcgcat gactcgcgcc tctttgtaat ccaccgccac aatgccgctg
    34801 tagcccaatg ccagcagttt ttccgccagc catggattac gaccaaactg cttagtcatc
    34861 agatacagtt caatgccgta aagccgcgcc gtctcaatca gccgcttacc gttttccagt
    34921 acctgatcca cgtcgatcac ccagctgtcc ggggcgatct tgccctgctg ccacaaactt
    34981 agtgcagcgg aaatcagcgc cggattctgg cgtttcaatg cttctacaaa catcttgttg
    35041 cctcgtttct gttccttgaa ttaaatattc atttttttga atatttaggt tgtgcgacgt
    35101 ggatttgtcg ggagcttcaa acctcttcgt tagcagccat ccataatcca taaaggttgg
    35161 ccagcaaata gccttcttca cacgggttaa cttccagtgt aaattccttc aacaacacct
    35221 gatgcaattg cacaacggct tgccagtggc tggattgcgc cagttctgcc agcaattcgg
    35281 cgtccagcgg ctctatttct tcaccacggc gactgcgcat cagtgcgctc gccatatgtg
    35341 tcatcgccat cgttccttgc tcactacgca ccggcagatg gcactctgtt tccagtacgt
    35401 tgacgacctg catcatgcct ttgcagatgt ccttatcaat aacgcctgca tcgcaaagca
    35461 ggttgagtct ggtttccata tcagatgcct ttcgtttaat aactcggttc ctgttcccca
    35521 ctcaccactc gttgttgatg ggcgagcagg gcgtgtttat caacaactgc acgcagcagt
    35581 tgttgcatcg ggtaatcatc ggcacgcgtc aggaccacgg cttcggtggc ctgtaaaaat
    35641 cgcggatcgt ctgtgagcgg cgtcgcttct aatcccagca tggtcagttc gttttccgcc
    35701 accacgttcc agataaccgc atcgacatcg cctttaacaa tgcgttgtaa actctcgtga
    35761 taagagagat cgacacgttc cacatcacta tcgccaaaaa agacatcggt catgattttc
    35821 tgatccgccg aacggttatc cagccccacg cgcttcacgt ttgcggactc gcctttacgg
    35881 caaatcaact ggtgctcgcc aacgtaggtg tgcggcccca gctccagcgc gaggcataac
    35941 cctttttgcg tgagataact ttccgccgcc agtcgggaaa caaccgccat gtcatacacg
    36001 ccgttaagta aacactccac gcgaatatcc gcgccgcgca tgtgcgcata gtaaaaagga
    36061 ataccatcaa actgagcttt caatccgctc gccaggcctt cgtacaaacg ggtatagggc
    36121 aagggcatcg cgcacaccac attgttgata tccacatgac tcagcagcgc tttgttatcc
    36181 atttccacca gataactgcc attacgccca cggcgttcta tccgtatcgc tccgcttgat
    36241 tccagcgttt tcaacgcggc ctgcgtcagg ccaacggatg aacggcattc agttgccagt
    36301 tcatctatgg ttttcagtcg attaccgcac ttttcaccca acaaataacg ggccagtgtc
    36361 gtgatgacga cgccttcttt tttgataaac gttcgacgca tagtaaattt tcaataaagt
    36421 gaatatttat atcttcataa aaatgaagat aagtggcaac tgcgagatgt ggaaacggcg
    36481 aggcgcttca cattttttcg gattattgca aaaagaggat aaaaaaaccg ggtttccccg
    36541 gtttttggca ttaatgttaa attacggctt cgccacaaaa ccaatcgctt cgtacaccgc
    36601 ttttagcgta cgggaagcgt gcgcgctggc tttttctgcg ccatctttca tcacctgttg
    36661 caggaaggct tcatcgttgc ggaaacggtg ataacgttcc tgcaattcag tcagcatacc
    36721 ggaaacggca tcagccactt cgcctttcag atgaccatac atcttgcctt cgaactgttt
    36781 ttccagttcc gggatgctct ggcccgttac cgcagaaagg atatccagca ggttggaaac
    36841 gcccgctttg ttctgcacgt cgtaacgaac taccggcggc tcgtcggagt cggtgaccgc
    36901 gcgtttgatt ttcttcacta ccgatttcgg atcttccagc aggccgataa cgttattgcg
    36961 gttatcgtca gacttggaca tctttttggt cggctccagc agcgacatta cgcgcgcgcc
    37021 agatttcgga ataaacggct ccggcacttt aaagatctcg ccatacagcg cgttgaaacg
    37081 ctgggcgata tcgcggctca gttccaggtg ctgtttctgg tcttcaccca ccggtaccag
    37141 attagtttga tacagcagga tgtccgcagc catcagcacc ggatagtcaa acagaccagc
    37201 gttgatgttc tcggcataac gcgcagattt atctttaaac tgcgtcatgc ggctcagttc
    37261 gccgaagtag gtatagcagt tcagtgccca gcctaactgt gcatgttccg gcacgtggga
    37321 ctgaacaaaa atggtgcttt tctcaggatc gataccacaa gccagataca aggccagcgt
    37381 atccagcgtc gctttacgca gcttctgtgc atcctggcgc acggtgatcg cgtgttggtc
    37441 aacgatacag taaatgcaat ggtagtcatc ctgcatgttt acccactgac gcagcgcacc
    37501 catgtagtta ccaatggtca attcacctga gggctgtgcg ccactaaaaa cgatgggctt
    37561 agtcattttt cgattcctga ttttcgctat gcggaagccc taatgcgggc agaaggtcat
    37621 ttatagactg ataaattaca tcaggctggc tgagatcgat agcctcgccg tagttatatc
    37681 cgtaggttaa gccaactgat gggcaacctg ccgcttttgc tgcctgaata tcattgcgtg
    37741 agtcgccaac aaacagcatc tgttgtgggg taattcccat ccgctcagcc accagtaaca
    37801 gcgggtccgg atgcggtttt ttgttttgca catcatcacc accaatcacc acgctgaagt
    37861 atttagcgat atctaaggct tccagcagcg gcgcgacgaa cggcgtcggt ttgttggtga
    37921 ccaggcccaa cggcaggcct ttagcctgca atgcgcccag cgtatcggca acgtgcggga
    37981 acaaaaacgt cccctcttcg gcaacctcgc catagtagcg atcgaatagt ttacgcagaa
    38041 tacgtacttg ttcttctgcc ggaatgtcgt catcaacggg cggtttaccc attgttttac
    38101 gcagagtcgc acgttcctga cgcgcccagg tcaatgcgcg ctccatcaga acatctgcgc
    38161 cgttaccaat ccaggttata acgcgttctt cacctgcgac gggcaactcc agcgcataca
    38221 gcgccatatc tactgcggta gccaagccag gagcactgtc caccagcgta ccatcgagat
    38281 caaaagcgac gccgcgaata tcttcaaact tattcatgac ttacctttgc cagttcactg
    38341 cgcatttcat caatgacttt tttgtagtct ggctggtcga agattgccga accggcgacg
    38401 aacatatccg cgcccgccgc agcgatttcg ccaatgttgt tcaccttcac gccaccgtcc
    38461 acttccagtc gaatgtcaaa gccagactcg tcgatacgac gacgtacttc gcgcagttta
    38521 tccagtgttt gcggaatgaa agactgaccg ccgaaaccag ggttgacgga catcagcagg
    38581 atcacatcca gcttatccat cacgtaatcc agatagctca gaggtgtcgc cgggttaaat
    38641 accagacccg ctttacagcc attttcttta atcagttgca gcgtgcggtc aacatgctcg
    38701 gaggcttctg gatgaaaggt aatgatgctg gcaccagcgg cagcgaaatc aggcacaatg
    38761 cgatcgaccg gtttcaccat caggtgtacg tcgatagggg cggtaatgcc gtagttacgc
    38821 aaggatttca gcaccattgg cccaatcgtc agattgggaa catagtggtt atccatgacg
    38881 tcaaaatgca cgacatcagc gccagctgcc agggcttttg cggtatcttc acccaggcgg
    38941 gcaaaatcag ccgacagaat tgagggggca atcaaatact gtttcatccg cttctccttg
    39001 agaattattt tttcgcgggt gaaacgactc ctggtttgta caaagccagc agttcgtcca
    39061 cctttttacg tgtgccgccg ttgctgctta tactgcgtcg aactttgacg acatgcaatt
    39121 ttgcgcgctg ataccactca cgcgttaaca tcgtatcgtg attggagatc agcactggaa
    39181 tatggcgatc aaccagacct tcagcgatct ccgccagatg cgcttgttgt tcaagcgtaa
    39241 aactgtttgt gtgatacgcc gtaaagttgg cggtcgcaga cagcggcgca taaggcggat
    39301 cgcaatagac gacggatgca tcatctgcgc gcgccatgct atcggcgtaa gactcacagt
    39361 agaaaaaggc attctgcgct ttttcagcga agtgatacaa ctctgcttcc gggaaatagg
    39421 gttttttgta gcggccaaac ggcacgttaa actcaccgcg cagattgtaa cgacacaggc
    39481 cgttgtaacc gtagcggttc aaatataaaa acagtaccgc ccgacggaac ggatcctggc
    39541 ttttgttgaa ctcttcgcgg aactgatagt aaacctcggc gcaatttgtt tcgggaacaa
    39601 acagctcgcg tgcggcctgt acgtactcat cagtacgcat cttcacaatg ttatagagac
    39661 tgatcaggtc gctattgata tcggcaagga tataacgaga aaagtcggtg ttgagaaaca
    39721 ccgacccggc acctacaaaa ggctcaacca gacattcgcc cttgggcaaa tgccgtttaa
    39781 tatcatcaag caggggatac ttgccccctg cccacttcaa aaaagcgcga tttttcttca
    39841 tgctgactaa ctaattacac cttctccggc tgtggagaaa gctccgacag catccagcgc
    39901 ttcaggcaac tcccgcaacg ataaaccatt gcgggagata accttgatta cttcagatcg
    39961 gcctgtacct gacgcagcgg tttcgcccac gggtttttgg cctggacatc ggctggcaat
    40021 gtagataccg cttttttcgc ctcttctttc gaggcgtaca cgccagaaac caggacatac
    40081 cacggctgac cattacgcgt cgtttcatag acaacgtagt ttttcagatt ctctttcttc
    40141 gcccaaccgt tcaggttgtc gtagttagag gaactgctca gctgcagagt gtaatggctg
    40201 gacggtgccg atttcaacga accaacatta cctgcggtct tccctccagc ggctggtgtt
    40261 gccgtggttt gcgccgggga tgccgtctgt actggagccg tagttgcggt ctcttttggt
    40321 gctggcgtag aggtcgcagc cggtgccttc gtgctcgcca caggagcagc tggttcagta
    40381 cgcttcggcg tctgtgctac cggcttcggc tccgttttca cggttgcttg cggttttttc
    40441 ggttcaatca ccgcctgctg acgagctggg cgcgtagtgg ccggacgttc agcggtttgc
    40501 gttttcgccg tgtcacgcga tgcattgcca ttgcgaacag gcgcgaccgt cgcgggttca
    40561 gtcggcaacg tggaattgac cgccacatta ttcaactgtt gctgattttg tggctgggtc
    40621 agcgcattgt tcaggtcacc ctgcacttca acacgttgtt gaccatccgt tgccaccggg
    40681 gtttgccctt gagtcggcgt agaagagatc ggcggcagag aaacatcctg ctgagtattt
    40741 tccgcagacg tggttcccgg cgctggctgc acgccattcg cctgatcggt cgcattgcca
    40801 gcaagatcaa tactcttctc gccagacgcg gtttgatcgc tggaagaggt cgagggggct
    40861 tttagcgcag aaccgatacc gatgatcaac agcaggagaa ccagaatgcc gacgcccatc
    40921 atcatatact gacgagaagc gggtttactg gctacttttt tgcgcttacg cggacgacgc
    40981 tctacgcgct cttcatccac ggtttcatct tcggattcgt caatttcttc ttcgagttcc
    41041 ggttcctcat tgcgctcttt ttgcgcacga gtcggacggc gatcgtcagt gtcatcaagt
    41101 tcaatatcat caaaattgat ctgcggttcg ccacgttcag tacgctcaga acgttcagaa
    41161 gattgacgag aacgaccagt acgacgatcg ctgggatcgg gtttcagctc gtcttctggt
    41221 ttgaattcat ccatttaaca ccccactaaa aggttaatgc ttaccacgtt gcaattaacc
    41281 tgaagctaat agaccgcttg ataagcggcc tgacctttct tgttgttacg ctgattgaca
    41341 atcggcaatg gcgttaagaa caagctcgtg cgaaacgccg ctgcgaactt cactcttacc
    41401 aattgccaac ggaagaatta agcgcatctc tcccgcaagg actttcttgt cacgcagcat
    41461 atgcggtaaa tacgcctgcg cggacatttc gcgcggccca ttgaccggta acccagcccg
    41521 cgtgagcagg gttataatac gctgcgtttc ggcagaacta aactgcccga gacgttccga
    41581 cgtccgcgcc gccatcacca tacccgcagc aaccgcttca ccgtgtaacc aattgccata
    41641 ccccatttca gcttcaatgg catgaccaaa ggtatgtccc agattcagta aagcacgtaa
    41701 cccggtttcg cgctcgtcgg cggcgacaac ttctgccttc agttcacaac aacggcgaat
    41761 acagtacgcc attgccggac cgtccagacg caacaacgca tccagattct cttccagcca
    41821 gttgaaaaac gcaccgtcaa gaataatgcc gtatttgatg acttctgcca gccccgacgc
    41881 taactcacgc gggggaagcg ttttcagaca gtcgagatcc accaccactg aagcaggctg
    41941 gtagaacgcg ccaatcatgt ttttaccgag gggatggttg accgcagttt tgccgccaac
    42001 ggaggaatcg acctgcgaca gtaacgtcgt cgggacttga atgaaacgta caccgcgctg
    42061 ataactcgcc gccgcaaagc cggtcagatc gcccactacg ccgccgccaa gcgccaccag
    42121 cgtagtatcg cgaccatgcg gtttttgtaa caacgccgta aagacggtat cgagtacagc
    42181 caggctttta tactgctcgc cgtcagggag gataacgcta tcgacgttaa cacccgcctg
    42241 ttcaagtacg ccgcggacct tatcgagata cagaggagcc agggtttcgt tggtaaccaa
    42301 catgacctgc tcgcccgatt tcagcggtaa gaatgaagct ggttcattaa acaaaccaga
    42361 tgcgatggta attgggtaac tacgttcccc gagagtaacg acaatcctct ccataacgcg
    42421 acatccacct taattactgt acccgcagac gagtgtatat aaagccagaa ttagttgctt
    42481 tccagcatgt gaataatctg gtttgcgacc actttagcgc tttgatcatc agtacgaatg
    42541 gtcacgtcgg caatctcttc atacagcgga ttgcgttcat tggccaacgc ttccagaact
    42601 tcacgcggcg gtgtttcaac gtgcagcaac gggcgttttt tatcacgctg cgtgcgtgca
    42661 agttgctttt cgatggtcgt ttcaagataa acgacaacgc cacgtgcgga aagacggtta
    42721 cgcgtttcgc gggatttcac agagccgccg ccagtagcca gcacaatacc ctgtttctcg
    42781 gtcaactcat tgatgacctt ttcttcgcga tcgcggaagc cttcttcgcc ttctaaatcg
    42841 aaaacccagc ccacatcagc tccggttcgt ttctcaatct cttgatcgga atcgtaaaat
    42901 tccatattga gttgttgagc taactggcgc ccaatagtgc tttttccggc acccataggc
    42961 ccaaccagaa agatattgcg tttctctgcc attttttcgg tactactaag actattcgtt
    43021 aatgataaac ccgcttcgct cagagagcgc cgcaggacat gaactgaaac ctcataagat
    43081 attgcgagag tcagactgaa aattatctca atactccagc gggtttggca actgaataaa
    43141 tcacccatcc ctctgcatat ctggtcgctg caagcgcgct gccttgctac caccgctctg
    43201 gcgataaatc accgggtaag attagcgtaa aaaaagacag caaaatgccg cctgaatgat
    43261 aaatcatcat catggaacgc aggcagcgaa gagcaccaac tctcaaatcg gtactccttg
    43321 tatgctaaat acctgcgcgc gtcaaataga tgaaacacgt tcagcgtaaa aacattaccg
    43381 cttttacggc tgtttactca ctggaaacca gtcgtggcgt gataaacacc actaactcgc
    43441 gtcgttcatc ttcttttccg tcatgacgaa ataattgccc gaaccaggga atgtcgccaa
    43501 gcaacggtac gctatcctga cccgatttat ttttacgggt aaaaatgccg cccagcgcca
    43561 acgtttctcc gcttttgacc tcgacctgcg tttcgatctc ctgcttatca atcgccaaca
    43621 cttcgccatc ggcctgctgt agcacctgcc ccggaacgtt ctggctgatg tgtaatttca
    43681 gccggatgcg acctttttgt aacaccgtgg gcgtaacttc catccccagg acggcctctt
    43741 taaattccac cgacgtcgcg ccactttccc cgctggaaac ctgatatgga atttcgctcc
    43801 cctgtttaat gctggcaggc tgaagatgag aggccagcag acgcggactg gcgataatat
    43861 ccagctgctg tttttgttcg agcgcggaaa gctcaagatc cagtaaacgt ccgttgatgc
    43921 gtccaatgtt aaaaccgaca tgcgttgtcg ccgtcgctac ggagaggtcg ctgccaagcg
    43981 tggtgacttg cccaacgcca ccagcttgtt gcgcatcagc caacgtccat ttcacgccta
    44041 actcacgcaa acttttttca ttaatggtga caatatgcgc cgacagctca acctgcccga
    44101 ccggcagatc catttgcgct acccactgtt caagcgcgct taacgccgtt ttgttatctc
    44161 gcagcaaaag gcgattggtg cgtttatcga cggtcatact ccctttggca ctcagtagct
    44221 tctcccccgc tttcgccagt tctcccgcgt cggcgtattg cagcgttata ctgcgatttt
    44281 ccagcggcag atttgcctgc gcccgcgcct gctccgcctc ctgacgggcg atattgtcat
    44341 tctgccaggc aacggaatgc accgagagaa tgttgccctc ctgacgcgtt atcagtccgg
    44401 cgcttttcac tacagtttgt agtgcttgct tccagggaac atctgttaga tgtaacgaca
    44461 ccgtaccgct gacgtctggc gacaccacca ggttcaactt ctcctgttca gccagcgcct
    44521 gcaacacctg agctaccgga acgtcatcca ccatcagcgt cactttttgc ggctttgccg
    44581 cctgtacgcc gggtatcagc atcaacagta gtgcggctat ccattgcttc atttgtatct
    44641 ccttgccgtt gccacaacca gtgtggcggt tcgcagtttg ccccggttcc cagcgttagt
    44701 gcgtctggcg tcagctgtaa gatcgtccag ccgttttcca gcacatcgtt ttgctgcacc
    44761 cgtcgccatt ttttttgccc atcttttatt acaccgatga tgcgctcgcc tcgccctacc
    44821 atcccctgat agcgccactg gctaagttcg ctaatccggc atagatcttc cggcggttta
    44881 aaagggtcac gcataccggt taaaaggcac aatgcaatac ctgccaacaa ccagcgttta
    44941 cccctcatgc ggcgtctcca gttgtagcgt gaacaaaaga tcatcacctt ccacgcttaa
    45001 cgaaaaacgg ctcacgctga cgttgcgctc tgccagccgt gtaaatgccg acggcactgc
    45061 ttcccacagc gttttcaacg ccaactcacc tccctgcgcg gatggatgcc aggaaaccag
    45121 ttgcgcgccg gataactgaa aatccagtgg cgaaaagggc agcgtttttt cttcgctgaa
    45181 gggagtggcg tctaccaggc gatacagtgc agcccactgc tggtgatgac tcgcccgtag
    45241 tcgaattaat gcgtcgctct cttcctggtg tgtcgatgac agaaaaatga gcgtaactaa
    45301 catcatcaac cagaatgccc agcaaagctg gcggaggcgg ggtgatgtgg cgaaccacca
    45361 gtcaaagagc atgtccatcg ctaaccttcc ttgttaactg atactcaaat tgccagcgtc
    45421 cctgcgcatc ctgctgcgtg gccccccgct gattgagatg aaaagaagca tcctggcgga
    45481 gtgacgtttc cagcgagttt aacgcggtaa tgcttgttgt cagccccttg atctccagcg
    45541 ttccctgctg ccagcttatc gttgtcagcc aggcatgttc gggtaaaagc gccgccagtg
    45601 cttccagcgc agattgccag tcgcgggtaa attgtcgctg gcgctgcctt tgcaaacgct
    45661 gttcgcgtaa ctgctggcgt tccagcaaac gtggtttcgt tatctgtaag ctgcgggcga
    45721 gttgttgttc cgcctgaagc aaaacggtgt ctacgcgtgc ttcggcgttg cttgtcagac
    45781 gcagtattag cgttatcccg acagccagca gcagaggcgc aacgaacatc agcaaccaga
    45841 aacgaagaaa agcggtccgg cgttgctgtc gccagggcaa aaaattaatt ggcgggttca
    45901 tcagtacacc ttcccaagcg ccagccccag cgcgatggca aagtctccac ctggcggcgg
    45961 tagcggcggc tgacgaacag aaaccgcctc ccaggggtca aatccgcctt cgccacatat
    46021 cgcgacgctt tcgggatcaa cggataacgc tgccgccagc tccttcgcgc tagttatccc
    46081 caccgccagt ttgcgccccc agctatagcg cgtcgcccac agccactgtt cgttatcacg
    46141 ccaggccaga cattgctgat gagaaggtaa aaaaggcagg aatcgctgta atgcactggc
    46201 atccggggtg atcgcgctaa catgaacacg caacctttct gccagagtaa gcagcgttgc
    46261 caactctttg ctttgcgcgg cagtcacgtt ataggcggga ctgagtgagt cttcgctgta
    46321 atcgaagcgc agggagtccg gatccatatc cagctcgcgg gccatcgtcc cggacagcca
    46381 ggccgtttgc tcccgctcac caagggacat cgacgggcgt ggaaatgacc gctgtaatgt
    46441 gcgactggcg ggaaacgcca acataatgtg atgacgctgc ggcagttcgc gactccacgg
    46501 taacaacgtg ttagccagcc gctgcgcatc aacaatccgc ccatctttga taatgtcgtg
    46561 cgccagcggc aaccgccacc agcgttgcaa aaagcattct tttgcgcccc gcacgatagc
    46621 aaccgctacc gcttcttgct gttgtaaatg caaaccaatt tgccagatct taaatgccat
    46681 tgtgatgatc tccttatcac ccgtcactct gacgggtata tcaatgcgtc tggcttgcct
    46741 ttatactacc gcgcgtttgt ttataaactg cccaaatgaa actaaatggg aaatttccag
    46801 tgaagttcgt aaagtatttt ttgatccttg cagtctgttg cattctgctg ggagcaggct
    46861 cgatttatgg cctataccgc tacatcgagc cacaactgcc ggatgtggcg acattaaaag
    46921 atgttcgcct gcaaattccg atgcagattt acagcgccga tggcgagctg atcgctcaat
    46981 acggtgagaa acgtcgtatt ccggttacgt tggatcaaat cccgccggag atggtgaaag
    47041 cctttatcgc gacggaagac agccgcttct acgagcatca cggcgttgac ccggtgggga
    47101 tcttccgtgc ggcaagcgtg gcgctgttct ccggccacgc gtcacaaggg gcgagtacca
    47161 ttacccagca gctggcgaga aacttcttcc tcagtccaga acgcacgctg atgcgtaaga
    47221 ttaaggaagt cttcctcgcg attcgcattg aacagttgct gacgaaagac gagatcctcg
    47281 agctttatct gaacaagatt tacctcggtt accgcgccta tggtgtcggt gctgcggcac
    47341 aagtctattt cggtaaaacg gtcgaccaac tgacgctgaa cgaaatggcg gtgatagccg
    47401 ggctgccgaa agcgccttcc acctttaatc cgctctactc aatggatcgt gccgtcgcgc
    47461 ggcgtaacgt cgtgctatcg cggatgctgg atgaagggta tatcacccaa caacagttcg
    47521 atcagacacg cactgaggcg attaacgcta actatcacgc gccggagatt gctttctctg
    47581 caccgtacct gagcgaaatg gtgcgccagg agatgtataa ccgttatggc gaaagtgcct
    47641 atgaagacgg ttatcgcatt tacaccacca tcacccgcaa agtacagcag gccgcacagc
    47701 aggcagtacg taataacgtg ctggactacg acatgcgcca cggctatcgc ggcccggcaa
    47761 atgtgctgtg gaaagtgggc gaatcggcgt gggataacaa caagattacc gatacgctga
    47821 aggcgctgcc gacctatggt ccgctgctgc ctgccgcagt caccagcgcc aatcctcagg
    47881 aagcgacggc gatgctggcg gacgggtcga ccgtcgcatt gagtatggaa ggcgttcgct
    47941 gggcgcgtcc ttaccgttcg gatactcagc agggaccgac gccgcgtaaa gtgaccgatg
    48001 ttctgcaaac gggtcagcaa atctgggttc gtcaggttgg cgatgcatgg tggctggcac
    48061 aagtgccgga agtgaactcg gcactggtgt cgatcaatcc gcaaaacggt gccgttatgg
    48121 cgctggtcgg tggctttgat ttcaatcaga gcaagtttaa ccgcgccacc caggcactgc
    48181 gtcaggtagg ttccaacatc aaaccgttcc tctacaccgc ggcgatggat aaaggtctga
    48241 cgctggcaag tatgttgaac gatgtgccaa tttcccgctg ggatgcaggt gccggttctg
    48301 actggcagcc gaagaactca ccaccgcagt atgccggtcc aattcgctta cgtcagggac
    48361 tgggccagtc gaaaaacgtg gtgatggtac gcgcaatgcg ggcgatgggc gtcgactacg
    48421 ctgccgaata tctgcaacgc ttcggcttcc cggcgcaaaa cattgtccac accgaatcgc
    48481 tggcgctggg ttcagcgtca ttcaccccaa tgcaggtggc gcgcggctac gcggtcatgg
    48541 cgaacggcgg cttcctggtg gacccgtggt ttatcagcaa aattgaaaac gatcagggcg
    48601 gcgtgatttt cgaagcgaaa ccgaaagtag cctgcccgga atgcgatatt ccggtaattt
    48661 acggtgatac gcagaaatcg aacgtgctgg aaaataacga tgttgaagat gtcgctatct
    48721 cccgcgagca gcagaatgtt tctgtgccaa tgccgcagct ggagcaggca aatcaggcgt
    48781 tagtggcgaa gactggcgcg caggagtacg caccgcacgt catcaacact ccgctggcat
    48841 tcctgattaa gagtgctttg aacaccaata tctttggtga gccaggctgg cagggtaccg
    48901 gctggcgtgc aggtcgtgat ttgcagcgtc gcgatatcgg cgggaaaacc ggtaccacta
    48961 acagttcgaa agatgcgtgg ttttcgggtt acggtccggg cgttgtgacc tcggtctgga
    49021 ttggctttga tgatcaccgt cgtaatctcg gtcatacaac ggcttccgga gcgattaaag
    49081 atcagatctc aggttacgaa ggcggtgcca agagtgccca gcctgcatgg gacgcttata
    49141 tgaaagccgt tcttgaaggt gtgccggagc agccgctgac gccgccgccg ggtattgtga
    49201 cggtgaatat cgatcgcagc accgggcagt tagctaatgg tggcaacagc cgcgaagagt
    49261 atttcatcga aggtacgcag ccgacacaac aggcggtgca cgaggtggga acgaccatta
    49321 tcgataatgg cgaggcacag gaattgttct gattaaaaag gcgcttcagc gcctttttaa
    49381 gtttgctgac aaagtgcact ttgtttatac cggatacggc gtgaacactt tacccgtcta
    49441 gcaacatcta gaaaaatcaa tatattgcag gagccgcaca ggcctgataa gcattatgca
    49501 tcaggcaaac ttcacgcatt tacaaccgcc cctgcccctt caaccattcg cgcacgagga
    49561 acagcgcgct gacattacgc gcttcgttga agtcagggtc ttccagcaaa tccatcatat
    49621 gcgccagcgg ccagcgcacc tgtggtagcg gctctggctc atcgccttcc agcgattccg
    49681 ggtagagatc ttgcgctacc acgatattca ttttgctgga aaagtaagac ggtgccatgc
    49741 tgagcttctt caaaaaagtc agatcgttcg caccaaatcc cacctcttct tttagctcgc
    49801 ggttcgcggc ttcgaagacg ctttcacccg gatcaattaa tcctttcgaa aaacctaatt
    49861 cgtaggattc agttcccact gcgtattcgc ggatcaggat caggtgatcg tccacaatcg
    49921 gcacaatcat cactgcttcc cggttggttg gacgcattcg ttcataaaca cgccgcacgc
    49981 cattgctgaa ctccagatcc acgctttcga cggtaaacag tcgggaacgg gctacagttt
    50041 caacattcag aatggtgggt ttttgtaatg atttgctcat cgtgggatct atgctgtgaa
    50101 atcagcggtt attgtgcgat atcggacacg ctttcggcaa tgtgaattgc atgttattta
    50161 catttatgta acttaataaa taatcgtcct caaatcaaat taaaagtcaa taggttgaaa
    50221 taactccagg aatttgctga tattccgcct tcagagggtt tgctatgatc agcggttact
    50281 gtgatgtgct taatgatgct taagttaaac tccacgcttg ccgatagcca accgcagaat
    50341 catgtattgt gtccggtgcg actgaccacg cctgacagac taagtaagat ggggaaagca
    50401 tgagcaccat tgtgattttt ttagctgctt tgctggcctg ctcactactt gcgggatggc
    50461 tgataaaagt gcgatccaga cggcgtcagc tgccctggac caacgccttc gcggatgcgc
    50521 aaacgcgtaa actcacacct gaagaacata gcgccgttga aaattatctt gagagcctga
    50581 cgcaggtatt acaggtgcct ggcccaacgg gagccagcgc ggcaccgatc tctctggcgc
    50641 tgaatgccga aagcaacaac gtcatgatgc tgacacacgc tatcacgcgt tacggcatct
    50701 ctaccgacga tccgaataaa tggcgttact acctcgattc ggtagaagtc cacctgcccc
    50761 ccttctggga acagtacatc aacgatgaga ataccgttga actgattcat accgattcgc
    50821 tgccgctggt tatttcactc aacggtcata cgctgcagga gtacatgcag gaaactcgcg
    50881 gctatgcctt gcaacctgtt ccgtcaacac aggcgtcgat tcgcggggaa gagagtgagc
    50941 aaatcgagct actcaatatt cgcaaagaaa cgcacgaaga gtatgcgcta agtcgtccgc
    51001 gcgggctgcg tgaagcgttg ctgattgtcg cctcattcct gatgttcttt ttctgcctga
    51061 ttaccccgga tgtatttgtt ccgtggctgg caggcggcgc gttactgctg ctgggcgcag
    51121 gtctgtgggg gctattcgcg cccccggcaa aatcctccct gcgggaaatt cattgtctgc
    51181 gcggtacacc ccgtcgttgg ggattgtttg gcgaaaacga tcaggaacag atcaacaata
    51241 tttcactcgg tattatcgac ctggtctacc ccgcacactg gcagccatac attgctcagg
    51301 atctcggtca acaaaccgat atcgatatct atctcgaccg ccacgtagtg cgtcaggggc
    51361 gatatctttc gctccatgat gaagtaaaaa actttccgtt acagcactgg ctgcgcagta
    51421 cgattatcgc tgcgggctcg ctactggtgc tgtttatgct gttattctgg atcccgctgg
    51481 atatgccgct gaaattcacc ctctcgtgga tgaaaggcgc gcagaccatt gaagccacca
    51541 gcgtgaaaca actggctgac gctggcgtgc gggttggcga tacattgcgt attagcggta
    51601 cgggaatgtg taatattcgc acttccggta cctggagcgc gaaaaccaac tcaccttttt
    51661 taccgtttga ctgctcgcag atcatctgga atgacgcccg ctcattgcca ttaccagaat
    51721 ctgaattggt caacaaagcg acggcattga ccgaagcagt taatcgccag ctgcacccta
    51781 aaccggaaga tgaatctcgc gtcagcgcct cattacgttc agcaattcaa aaatccggca
    51841 tggtattgct tgatgatttt ggcgacatag tactgaagac agcggatctg tgttctgcca
    51901 aagatgactg tgtgcgactg aaaaatgcgc tggtcaatct cggcaacagt aaagactggg
    51961 acgcgctggt aaaacgcgcc aacgccggga agctcgatgg cgtgaatgtg ttattacgcc
    52021 cggtgagtgc ggaatcgctg gataacctgg tggcaacctc caccgcgccg tttatcacgc
    52081 atgaaacggc gcgagcggca caatcactaa acagtccggc ccccggcgga ttcctgattg
    52141 tcagcgacga aggcagcgat tttgttgatc agccctggcc ttcggcatca ctttacgact
    52201 acccgccgca agaacagtgg aacgctttcc agaaactggc acaaatgctg atgcatacgc
    52261 cgtttaacgc cgaaggtatc gtcacaaaaa tcttcactga cgccaatggt acgcagcata
    52321 taggccttca tccgatcccg gatcgttccg gcctgtggcg ctatctcagc accacgctgc
    52381 tgctactgac gatgctgggt agcgccattt ataatggcgt acaggcctgg cgtcgttacc
    52441 agcgtcatcg tactcgcatg atgaagattc aggcctatta tgaaagctgc ctgaacccgc
    52501 aactgatcac cccttcagaa agccttatcg aataacacgt ttgcgcggca ggttatgcta
    52561 ccctgtcgcg caaattgctt cactctggag atttccctca tgcatatcaa cattgcctgg
    52621 caggacgtag ataccgttct gctggatatg gacggcacgt tgctcgacct cgccttcgat
    52681 aattatttct ggcaaaagct ggtgcctgaa acatggggcg cgaaaaacgg ggttacgcca
    52741 caggaagcga tggaatatat gcgccagcaa tatcacgacg tacagcatac gctaaactgg
    52801 tactgtcttg attactggag tgagcaactg ggtctggata tctgtgcgat gaccaccgag
    52861 atgggaccgc gtgccgtact gcgtgaagat accattccgt ttcttgaggc actgaaagcc
    52921 agcggtaagc agcgaatttt gctcaccaat gcgcatccgc acaacctggc ggtaaaactt
    52981 gagcataccg gtctggacgc acaccttgat ttattacttt ccacccacac atttggttat
    53041 ccgaaagagg atcagcggtt atggcatgcg gtggccgaag ctacgggtct gaaagctgaa
    53101 agaacgctgt ttattgatga cagcgaagcg attctcgatg ctgccgcgca atttggtatt
    53161 cgttactgcc tcggcgtgac taatcctgac tccgggattg ccgagaaaca gtatcaacgc
    53221 catccgtcac tgaatgacta ccgccgcctg atcccctcgc taatgtgaag gagacgccat
    53281 gaaagaaaaa cctgctgttg aggttcgact ggataaatgg ctatgggctg cccgttttta
    53341 taaaacccgc gcgctggccc gtgaaatgat tgaaggcggt aaggtgcatt acaacgggca
    53401 gcgcagcaag ccgagcaaaa tcgtcgagct gaatgccacg ctcactctgc gccagggaaa
    53461 tgacgaacgc acggtgattg taaaggcgat tactgaacag cgtcgccccg ccagcgaggc
    53521 agccttgctg tatgaagaga ctgcggaaag tgtagagaaa cgcgaaaaaa tggcgctggc
    53581 acgtaaactt aatgccttaa ccatgccgca cccggaccga cgcccggaca aaaaagagcg
    53641 ccgcgacctg ttacgattta aacacggcga cagtgaataa ctgtcacctg caagagagat
    53701 gattatgccg caacatgacc aattacatcg ctatctgttt gaaaactttg ccgtgcgcgg
    53761 cgaactggta accgtttcgg aaaccctgca acagatcctt gagaatcacg attatccgca
    53821 gcccgttaaa aacgtgctgg cagaactgct ggttgcgacc agcctgttaa ccgctacgct
    53881 gaagtttgat ggtgatatca ccgtacagct gcaaggcgac ggtccgatga atctggcggt
    53941 tattaacggt aacaacaacc agcagatgcg cggtgtagcg cgcgtgcagg gagaaattcc
    54001 agaaaatgcc gacctgaaaa cgctggtcgg caatggttac gtggtgatca ccatcacccc
    54061 gagcgaaggt gaacgctatc agggcgtggt tggtctggaa ggtgataccc tggcggcctg
    54121 cctggaagat tactttatgc gttctgaaca gctgccgacg cgcctgttta ttcgcaccgg
    54181 cgacgtagac ggcaaaccgg ctgcaggcgg aatgttgttg caggtaatgc ctgcgcaaaa
    54241 tgcccagcag gacgactttg accacctggc gacgctaacc gaaaccatca aaaccgaaga
    54301 attgttgacc ttaccggcaa acgaagtgtt gtggcgtttg tatcacgaag aagaggtgac
    54361 ggtttacgat ccgcaggatg tggagttcaa atgcacctgt tcgcgtgaac gttgcgccga
    54421 cgcgctaaaa acgctgccgg atgaagaagt cgatagtatc ctggcggaag atggcgaaat
    54481 tgacatgcat tgcgattact gcggtaacca ctatctgttc aatgcgatgg atattgctga
    54541 aatccgcaat aacgcctctc cggcagatcc gcaagttcat taatgagtat gtccggcagt
    54601 gagggctctg ccggatttta cgacttaatg gcctgatgcg ctgcgcttac caggcctatg
    54661 aaataaatcg caaattattt tggtcagata cagcgtcgta tccgttaaaa gcgttgtcac
    54721 ttacttaagt ttatttccag cgtttcacca tctaccgtca gatcgcgcca cgctttcggc
    54781 ctcgtcccca gttttccatc cttcgcagct gtgagtaatt tttctttcgt tatttcgttc
    54841 ggaagtaaac tcatatcaag cgcgtcccat tcttttctgt ctgagtcaaa gccatagaca
    54901 atgactctgt catcattaaa agcaaacagg atccgctccg cctgaccatc actattcaga
    54961 tcttgctcga ccaggacgca ggcgtctttt tcaatgcagg tcatcacgtt atagcgatcc
    55021 tggattaagg ccgaccagaa tgtcgcatcc ggtttaacag aaccaggggc aattaacaca
    55081 ttttcggcta atactttttc cgatatctgt tgctgaagat gttgctctcc atctaacgcc
    55141 atcaacagat cccgcgcgcg tttcgggtct ttcataaacc cggcatcgct tttcagcgac
    55201 tcaagcgccg cacgtccata gcgaccgctc tgttcgagca tatagagact tacctggtct
    55261 ggcgtgtttt tgccgctctg ataacgcgcc atatgactgt tcacgctaat gcgcatactg
    55321 tccagcaccg gagaattaag aagcaccagt attaccaaca ccaataacga aaccgcaagg
    55381 ttcactttgc cctgaagaac aagggggttt tgccctttac gccagacgat actgacaaaa
    55441 taccccagcg accataccag taacaccagc accgccagcg cgccctgcaa gcggtcgaca
    55501 gtccagccgt actgagcgac ccgcagccat aacgcccagg cggcgacgaa cacatacagc
    55561 ggagcagcca gcagagcggt tttaatcagg caacgcaacg gccccgtcca gggaagtgac
    55621 gatttttgcg gatcgcggac aatagccatt aagatcaatt gcaaaaaggc cagcgtcaac
    55681 agcaacccgg cggcggaaat gtggcgagaa atcgcgctca ggcccgtaaa cggcagggtg
    55741 atgataaaca tcagggttag caatgatact aacggcagca accccgtggc gattaacgtg
    55801 aacaactttt gaatagagtc gattaaacgt gactgtgtac gcgcgaggat caccgccagt
    55861 gcggtaacca gacctaacgt gagataaata aaccagtcgg ttgcaaaaaa gagcgttttg
    55921 aaaaacgtga tgccaacaag tttaaacaac tcaccccaca gtaaaagcac cagccacgtc
    55981 aggccattgg cgagaaaaat caccagtaat ataagtacgt tatgccatac tgactggtaa
    56041 aaatagccat agcggctact gtcattgcgg atgcgaaggc tttgttgtat ccacggtagc
    56101 aacaacattg ccatcagcag cagatagcag ccaaaatccc aaagcgcctt ttctgctctc
    56161 caggggttca tgccatcaat ttgccacttt agccaaccgc tcatccctag cgtggcaata
    56221 aacaccagcg ccagccatcc ccaaaggcgc ttctgtttaa aggagatcac ggagaagagt
    56281 aaaacggatg aaaacgcgac ggtcgcaggc acgccataaa caatccaact gagatttttt
    56341 ccggcaagcc aggcgatcag taggtagcaa accagaccct gaagtaatcc ggtagcaatc
    56401 atcccccagc gtgttgcggg tgaaagttca acgttatcca cgttagttat ccagaatcaa
    56461 aaagtgggtt aattatcgca tccgggccgt agtattttgc ttttttcaga aaataatcaa
    56521 aaaagttagc gtggtgaatc gatactttac cgattgaatt tgcatcaatt tcattcagga
    56581 atgcgattcc actcacaata ttcccgccac ataaaccaag atttaacctt ttgagaacat
    56641 tttccacacc taaaatgcta tttctgcgat aatagcaacc gtttcgtgac aggaatcacg
    56701 gagttttttg tcaaatatga atttctccag atacgtaaat ctatgagcct tgtcgcggtt
    56761 aacaccccca aaaagacttt actattcagg caatacatat tggctaagga gcagtgaaat
    56821 gcgcgttaac aatggtttga ccccgcaaga actcgaggct tatggtatca gtgacgtaca
    56881 tgatatcgtt tacaacccaa gctacgacct gctgtatcag gaagagctcg atccgagcct
    56941 gacaggttat gagcgcgggg tgttaactaa tctgggtgcc gttgccgtcg ataccgggat
    57001 cttcaccggt cgttcaccaa aagataagta tatcgtccgt gacgatacca ctcgcgatac
    57061 tttctggtgg gcagacaaag gcaaaggtaa gaacgacaac aaacctctct ctccggaaac
    57121 ctggcagcat cttaaaagcc tggtgaccaa acagctttcc ggcaaacgtc tgttcgttgt
    57181 cgatgctttc tgtggtgcga acccggatac tcgtctttcc gtccgtttca tcaccgaagt
    57241 ggcctggcag gcgcattttg tcaaaaacat gtttattcgc ccgagcgatg aagagctggc
    57301 aggtttcaaa ccagacttta tcgttatgaa cggcgcgaag tgcactaacc cgcagtggaa
    57361 agaacagggt ctgaattccg aaaacttcgt ggcgtttaac ctgaccgagc gcatgcagct
    57421 gattggcggc acctggtacg gcggcgaaat gaagaaaggg atgttctcga tgatgaacta
    57481 cctgctgccg ctgaaaggta tcgcttctat gcactgctcc gccaacgttg gcgagaaagg
    57541 cgatgttgcg gtgttcttcg gcctttccgg caccggtaaa accacccttt ccaccgaccc
    57601 aaaacgccgc ctgattggcg atgacgagca cggctgggac gatgacggcg tatttaactt
    57661 cgaaggcggc tgctacgcaa aaactatcaa gctgtcgaaa gaagcggaac ctgaaatcta
    57721 caacgctatc cgtcgtgatg cgctgctgga aaacgtcacc gtgcgtgaag atggcactat
    57781 cgactttgat gatggttcaa aaaccgagaa cacccgcgtt tcttatccga tctatcacat
    57841 cgaaaacatc gttaagccgg tttccaaagc gggccacgca actaaagtta tcttcctgac
    57901 cgcagacgcc tttggtgtgc tgccgccggt ttctcgtctg actgccgatc aaacccagta
    57961 ccacttcctc tctggcttca ccgccaaact ggctggtaca gagcgcggca tcaccgaacc
    58021 gacgccaact ttctccgcct gcttcggcgc ggcattcctg tcgctgcacc cgactcagta
    58081 cgcagaagtg ctggtgaaac gtatgcaggc ggcaggcgcg caggcgtatc tggttaacac
    58141 cggctggaac ggcactggca aacgtatctc cattaaagat acccgcgcca ttatcgacgc
    58201 catcctcaac ggttcgctgg ataacgcaga aaccttcact ctgccgatgt ttaacctggc
    58261 gatcccaaca gaactgccgg gcgtagacac gaagattctc gatccgcgta acacctacgc
    58321 ctctccggaa cagtggcagg aaaaagctga aacgctggcg aaactgttta tcgacaactt
    58381 cgataaatac accgacaccc ctgcgggtgc cgcgctggta gcggctggtc cgaagctgta
    58441 atcagctgaa atctcattga ccccttatcc aaacggataa ggggtttttt tatatctaat
    58501 tagctaccaa tatgtacacg gtacacttat aattatcaat agaccgcagg atgcattatg
    58561 ttcacgttta ttgaactaca aggatttagc aaacgccgcc agttattgtt acctgatgat
    58621 gagttccggg cttttcagga actactcatt caagatccca ctgccggtga cattatcgca
    58681 ggcacaggca gttttagaaa aattcgctgg aaccgttctg gaatggggaa acggggtggc
    58741 attcggataa tttattacaa cgtaacacgc aatggccgaa tttatctggc gttgatttat
    58801 cctaaaaatg aacaggatga tttaacagaa gaacaaagaa aggcattgaa actattgtcc
    58861 gagaagctgc tttaatgttg aagctccaca aattccacca tggccgaaat gccaacggag
    58921 gcattgaaaa cagatgaaag acgaattatt tgctgatctt cttgccagcg cagaagaaat
    58981 ggtacgcatc gaaaagggtg aagaagcgcc tgaaccagag cacgtccata cttttagcga
    59041 aatcgatgta aaagcgatcc gtgaagcaac gggtttacgt cagcaagatt ttgctgtcgc
    59101 cgtaggggta agttatgacc tggtcaaaag ctgggaaacc aaacgccgtc aacctaccgg
    59161 agcaccccga aaattgctcc tgcttttgca agcgaatcct ttcattatca atcaattgaa
    59221 aacaatctaa aaacaggagg caccttcgcc tcccgtttta tttacccttc ttttgtcgca
    59281 ccctgcgccc gcgttaccgg cacaggcagc caggcgcgaa tggaaagccc gccccgttcg
    59341 ctggtgccaa gctccagcat cccgttatgg ttatccacga tacgctgcac aatcgccagc
    59401 cctaatcccg tgccgctaat ggtgcgcgca ctgtcgccgc gaacaaacgg ctggaacagg
    59461 tgcttacgtt gttccggcgc aattcccgga ccgtcatctt ccacctggaa ccaggcgcga
    59521 ttcggctccg ttccgctgct gactttgatc cagccattgc cgtaacgggc ggcgttgacc
    59581 accatattcg ccaccgcacg tttgatcgac agcgggtgca ttttcacctc aatgctgccg
    59641 gggtaaagcg cggtttcaat ttcccgctca tagccacttt cggcggcaat cacctcaccg
    59701 agcactgcgt tgagatccgc catttccatc ggcatctcct gcccggtgcg caggtagtcg
    59761 ataaactgct caatgatggc gttgcactct tcgatatctt tattgatcga ttctgccagg
    59821 tagccatcct gttcgctcat catctcggta gccagacgaa tgcgcgtcag cggcgtgcgc
    59881 agatcatgac ttacccccgc catcagcagc gtgcgatcat ccgccagttg cttaacacca
    59941 gccgccatat ggttaaaggc gcgggtaacg gaacgcacct ccgaagcgcc atattcacgc
    60001 agcggtggcg gtataatccc tttaccgacc tgcaaagcag catgttcgag atcaaccaac
    60061 ggtcggttct ggatacgaat aaacaaccac gccccgccta tcgccaatag cataatcgcc
    60121 agcgtatagc ggaacagcgg agaaaaatcg ccctgatgaa tttcggtcag cggcacgcgc
    60181 acccagatat tgggcgacag ccaggttttc agccagacga caggcgaact tttgttgacc
    60241 tcaacgcgta cttccgtcgg gccgcccagt tgctgcgcca tctgatggct taagaattca
    60301 tagtgttgtg cccaacgcag acctgcctct tcagcagctt cgttggagta gagagagatc
    60361 cccagctcac ggtagatctc ccgacggaaa gcgggaggca caaccaactg cgtaccgtcc
    60421 tccagttgca gtttgtcggt catcaacata cgcacttcgt acgcgaggac tttattaaac
    60481 tgctggaggc tcggcaaaat cgcgaagttc agcaccacca gataagtcgt caccaggctg
    60541 acgaacagca aggtgacgat gagcaataac gtacgggcaa atgaacttcg tggcgagaag
    60601 cgcaatcgcc tcatgcttta gaaccgtccg gtacaaagac gtagcccaga ccccagacgg
    60661 tctgaatgta acgcggatgc gccggatctt cttccaccat gcggcgtaga cgcgaaatct
    60721 gcacgtcgat ggagcgttcc attgcggagt attcacggcc acgggcaagg ttcatcagct
    60781 tatcgcggga gagcggctca cgcggatggc tgaccaatgc cttcagtacc gcaaactcac
    60841 cgctggtgag cggcatcggc tcatcttcgc ggaacatttc acgcgtaccg aggttaagtt
    60901 tgaacttacc gaaagcaatt accgcctctt cctgtgacgg cgcgcccggc agttcgttgg
    60961 cctgacgacg cagcaccgca cgaatgcggg ccagcaattc acgcgggtta aacggttttg
    61021 gaatgtagtc gtcagcgcca atctccaggc caacgatacg gtccacttct tcccctttcg
    61081 ccgtcaccat aatgatcggc atcgggttgc tctggctacg aagacgtcgg caaatcgaca
    61141 agccatcttc accaggtaac atcaaatcca gtaccataag atggaaagat tcacgagtca
    61201 gcaggcgatc catctgttct gcattagcga cgcttcgaac ctggaagcct tgttcggtga
    61261 gataacgttc cagcagcgcg cgcaggcgca tatcgtcatc gaccaccaga atcttgtagt
    61321 tctcttgcat tgtttgtact cccaaaggtt cgcaacaatt tgtaagcgtg tattcttaaa
    61381 aaagctcacg ttcgtcacca gctaaatctg gtatgaattt cagcctaaat tgttacaaag
    61441 catattaaac agcagcttaa gtatacaatt tattcggcga aacattattg attctgttga
    61501 tatgatcacg ttatacccaa tgtgcgcatt atcaaacaga caaagggaat caacgagatg
    61561 aaaacgcccc tggttacccg ggaagggtat gaaaaactca aacaagagct taattatctc
    61621 tggcgtgaag aacgcccgga ggtcacaaaa aaggtgacct gggccgcaag tctgggcgac
    61681 cgcagcgaaa atgctgacta tcagtataat aaaaagcgcc tgcgtgaaat cgaccgtcgc
    61741 gtgcgctatc tcactaaatg catggaaaat ctcaaaatcg tcgattactc cccgcagcag
    61801 gaaggcaaag tcttttttgg tgcgtgggtg gagattgaaa acgacgatgg cgtgactcac
    61861 cgtttccgta ttgtcggcta cgatgaaatt tttggtcgta aagattacat ctctatcgat
    61921 tccccgatgg ctcgcgcatt gttgaaaaaa gaggtgggcg atttggcggt ggtgaatacc
    61981 cctgccgggg aagcgagctg gtatgttaat gctatcgagt acgtgaaacc gtaaggaaga
    62041 gtcttaacct cctgccgatg gctggcattt ttgccagcca gtccgtataa ctatcccctg
    62101 atttttgatc cgaaaagatg aactcaaacc atgatgaatg attcgttctg ccgcattatt
    62161 gcgggtgaaa ttcaggcgcg cccggaacag gttgacgctg ccgttcgcct gcttgacgaa
    62221 gggaataccg tgccgtttat cgcacgttat cgtaaggaaa tcaccggcgg tctggatgac
    62281 acgcagctgc gtaatctgga aacgcgtctg agctatctgc gcgagctgga agagagacgt
    62341 caggcgatcc tcaaatccat ttccgagcaa ggcaaactca ctgacgagct ggcgaatgcc
    62401 atcaacgcca ccttaagcaa aaccgaactc gaagacctct acctgcccta caaacctaaa
    62461 cgccgcaccc gcgggcaaat cgccattgaa gcagggcttg agccgttggc cgatctgctg
    62521 tggagcgatc cgtcacacac gccagaagtc gccgctgcac aatatattga tgccgataaa
    62581 ggcgtggcag ataccaaagc cgcgctggac ggcgcgcgct atatcctgat ggaacggttt
    62641 gccgaagatg ccgcgctgct ggcaaaagta cgtgattatc tgtggaagaa cgcgcattta
    62701 gtttctacgg tggtgagtgg taaagaagag gaaggggcga aattccgcga ctatttcgat
    62761 catcacgaac cgctgtcaac ggtgccttct caccgcgcgc tggcgatgtt ccgtggtcgt
    62821 aacgaaggcg tacttcagct ttcgctgaat gccgatccgc agttcgatga gccgccaaaa
    62881 gagagctatt gcgagcaaat cattatggat catctcggcc tgcgcctgaa caatgccccg
    62941 gcggatagct ggcgcaaagg cgtggtgagc tggacgtggc gcatcaaggt gctgatgcat
    63001 ctggaaaccg aactgatggg caccgtgcgc gaacgcgcgg aagatgaagc aatcaacgtc
    63061 tttgcccgta acctgcacga tctgctgatg gcggccccag ccggactgcg tgcaacgatg
    63121 ggcctcgatc cgggtctgcg taccggggta aaagtagcgg tggtcgatgc aactggcaaa
    63181 ctggtggcga ccgataccat ttacccgcac accggacaag ccgcaaaagc agcgatgacc
    63241 gttgctgccc tgtgtgaaaa gcataatgtt gaactggtgg cgatcggcaa cggtacagct
    63301 tcccgcgaaa ctgagcgttt ctatctcgac gtgcagaagc agttcccgaa agtgaccgcg
    63361 cagaaagtga tcgtcagcga agctggcgcg tcggtttact cggcttccga gctggcagcg
    63421 caggagttcc cggatctcga cgtttcgctg cgtggcgcgg tgtctatcgc ccgtcgtttg
    63481 caggatccgc tagcggagct ggtgaaaatc gatccgaaat ctatcggcgt aggtcagtat
    63541 cagcatgacg tcagccagac gcagctggcc cgcaaactgg atgcagtagt agaagactgc
    63601 gtaaacgccg ttggcgtcga tctcaacact gcttctgttc cgctgttaac tcgcgtggcg
    63661 ggcctgacgc gcatgatggc gcaaaacatc gttgcctggc gcgatgagaa cgggcagttc
    63721 cagaaccgtc agcaactgtt aaaagtgagc cgtctggggc cgaaagcctt cgaacagtgt
    63781 gcgggcttct tgcgcattaa ccatggtgat aacccgctgg acgcctctac cgttcacccg
    63841 gaagcctatc cggtggtgga acgcattctg gcggcgacgc agcaggcact gaaagatctg
    63901 atgggtaaca gcagcgagct gcgtaacctg aaagcgtctg actttactga tgagaaattc
    63961 ggtgtgccga cagtaactga catcatcaaa gagctggaaa aaccaggtcg cgatccgcgt
    64021 ccggaattta aaaccgctca gtttgccgat ggcgtcgaga caatgaacga cctgcaaccg
    64081 ggtatgatcc tcgaaggcgc ggtgaccaac gtcaccaact ttggcgcgtt tgtcgatatt
    64141 ggcgtgcatc aggacggcct ggttcacatc tcttcattgt cgaacaagtt tgtggaagat
    64201 ccgcataccg tggtgaaagc gggcgacatt gtgaaggtga aagtgctcga agtcgatctt
    64261 cagcgtaaac gtatcgccct gactatgcgt ctggacgagc agcctggcga aaccaacgcc
    64321 cgtcgcggcg gcggtaatga acgcccgcaa aacaaccgcc cggcagccaa accgcgtggg
    64381 cgtgaagcgc agcctgccgg taacagcgcg atgatggatg cgctggcggc ggcaatgggc
    64441 aaaaaacgtt aaacgccctg atagacaaac gtaaacctgc cagataaagc gttcacgccg
    64501 catctggcaa cgaacgcctt gcctgacata aaagtgccgg agaatatctc cggccttttt
    64561 attccacaac caaactctca atatatcccg ttaatattta tcacttcatt aacaactgaa
    64621 accttaatta aacattaacc agtcctggta attcgctatt cgaattatat tttcgctgcg
    64681 atataacctt gagccacatc aacattgagt cagattatta ttcaaaccaa cattcgcaca
    64741 cattttaagt attgctgata gaaaccattc tcattatcat tgtgttgttg attatttaat
    64801 ctctccttcg ttggcaaatc atctggtctc atgtcgctgt caaacgcccc atgaggtagt
    64861 tatccagtta atgagaaaca agtaggcacc tatgcaatac actccagata ctgcgtggaa
    64921 aatcactggc ttttcccgtg aaatcagccc ggcatatcgc caaaaactgc tttctctagg
    64981 catgttacct ggctcctctt ttaatgtggt gcgcgtcgct ccactcggcg accccattca
    65041 tatcgaaacc cgtcgtgtga gcctggtatt acgcaaaaaa gatctggcct tattagaagt
    65101 ggaagcggtt tcctgttaat acggtgataa caacaatgaa aaaattaacc attggcttaa
    65161 ttggtaatcc aaattctggc aagacaacct tatttaacca gctcactggc gcacgtcagc
    65221 gtgtaggtaa ctgggctggt gttaccgtcg aacgtaaaga agggcaattc tccaccaccg
    65281 atcatcaggt cacgctggtg gacctgcccg gcacctattc tctgaccacc atctcatcgc
    65341 agacctcgct cgatgagcaa atcgcctgtc actatattct gagtggcgac gccgacctgc
    65401 tgattaacgt ggtggatgcg tctaaccttg agcgtaacct gtacctgacg ctgcaactgc
    65461 tggaactcgg cattccctgc atcgtggcac tgaacatgct cgacattgcc gagaagcaaa
    65521 atattcgtat tgaaattgat gctctgtcgg cgcgtcttgg ctgcccggtg atcccgctgg
    65581 tttcaacccg tggtcgcggt attgaagcgc tcaagctggc gattgatcgc tataaagcta
    65641 acgagaatgt ggaactggtg cattacgcac agccgctact caacgaagca gattcactgg
    65701 caaaagtgat gccttccgac atcccgctga aacaacgtcg ctggctgggc ctgcaaatgc
    65761 tggaaggcga tatctacagc cgcgcctacg ccggtgaagc gtcgcagcat cttgatgccg
    65821 ccctcgcccg tctgcgtaat gagatggatg atccggcgct gcacattgcc gatgcgcgtt
    65881 accagtgcat tgctgccatc tgtgatgtgg taagcaacac cctgacggca gaacccagcc
    65941 gtttcaccac tgcggtagat aaaatcgtgc tcaaccgttt cctcggtctg ccgattttcc
    66001 tctttgtgat gtacctgatg ttcctgctgg ctatcaacat cggcggggcg ttacagccgc
    66061 tgtttgacgt cggctccgtg gcgctatttg tgcatggtat tcaatggatt ggctacacgc
    66121 tccacttccc ggactggctg actatcttcc tcgcccaggg cctgggtggc ggtattaaca
    66181 ccgtgctgcc actggtgccg cagatcggca tgatgtacct gttcctctcc ttccttgagg
    66241 actccggcta tatggcgcgt gcggcgtttg tgatggaccg tctgatgcag gcgctgggct
    66301 tgccggggaa atcctttgta ccgctgatcg ttggtttcgg ttgtaacgta ccgtcggtaa
    66361 tgggcgcacg tacgcttgat gcaccgcgtg aacgtctgat gaccatcatg atggcaccgt
    66421 ttatgtcctg cggcgcgcgt ctggctatct tcgcagtatt tgcggctgcc ttcttcgggc
    66481 agaacggtgc gctggcggtc ttctcgctgt atatgctcgg tatcgtaatg gcggtgctga
    66541 cgggcctgat gctcaagtac accatcatgc gcggtgaagc gacgccgttt gtcatggagc
    66601 tgccggtcta ccatgtacca cacgttaaaa gcctgattat ccagacctgg cagcgtctga
    66661 aaggcttcgt tctgcgtgct ggtaaagtga ttatcatcgt cagcattttc ctgagtgctt
    66721 tcaacagctt ctctctgagc gggaaaatcg tcgataacat caacgactcg gcgctggctt
    66781 ccgtcagccg ggtgatcacc ccagtcttca agccgattgg cgtgcatgaa gataactggc
    66841 aggcaacggt tggcctgttt acaggcgcaa tggcgaaaga agtggtggtg ggtacgctca
    66901 acaccctcta caccgcagaa aatattcagg acgaagagtt caatccggcg gaatttaacc
    66961 tcggtgaaga gctgttcagt gcggtagatg aaacctggca gagcctgaaa gacaccttca
    67021 gccttagcgt actgatgaac ccgattgaag cgagcaaagg cgacggcgaa atgggtaccg
    67081 gggcgatggg tgtgatggat cagaaattcg gtagcgcagc tgccgcatac agctacctga
    67141 ttttcgtcct gctgtacgta ccgtgcatct cggtgatggg tgctatcgcc cgtgaatcaa
    67201 gccgtggctg gatgggcttc tccatcctgt gggggctgaa tatcgcttac tcgctggcaa
    67261 cattgttcta tcaagtcgcc agctacagcc agcatccaac ttacagcctg gtgtgcattc
    67321 tggcggttat cctgtttaac atcgtggtta tcggtctgct gcgccgcgcg cgtagccggg
    67381 tgaatatcga actgctggca acgcgcaaat cggtaagcag ttgctgcgca gccagcacca
    67441 ccggtgattg ccattaatgg cttcacttat tcaggtacgt gatttgctgg cgttacgggg
    67501 ccgtatggaa gcggcccaga taagccaggc attgaatacg ccgcagccaa tgattaacgc
    67561 catgctgcaa cagctggaaa gcatgggcaa agccgtgcgg attcaggaag aacctgacgg
    67621 ctgcctctct ggcagttgta aaagctgccc ggaaggcaaa gtctgtctgc gcgagtggtg
    67681 ggcgctgcgt taaccatact ccatcgcctg ttttgaaaag cccggtatgc gtttgcatcc
    67741 gggctttttt gcgtgcggct ttccataaaa atgcaattct tgcagcgcga cgtaagttcc
    67801 tctgaaagcg tctcgcaggg atgaaaactg gctaatgcac aggtgtggag tggcgcgtag
    67861 agtcgcggca ttcaaataac aggtgaagga acgccatgag caaaaagcag agttccaccc
    67921 cacacgatgc gctgttcaaa ctctttttac gccaaccgga gacggcacgc gattttcttg
    67981 cgtttcattt accggcaccc attcacgcgc tctgtgatat gaaaaccctc aagctggagt
    68041 cgagcagctt tattgatgac gatctgcgtg aaagctattc cgacgtgctg tggtcggtga
    68101 aaacggaaca agggccagga tacatctatt gtctgattga acatcaaagc acctcaaaca
    68161 aactgatcgc atttcgcatg atgcgctacg ccattgccgc aatgcaaaat cacctggatg
    68221 ccggatacaa aaccttgccg atggtggtgc cattgttgtt ttaccacggt attgaaagcc
    68281 cctatcccta ttcgctgtgt tggcttgatt gtttcgccga tcccaacctg gcaaggcagc
    68341 tttatgcctc tgcatttccg ctgattgatg tcaccgtcat gcctgatgat gaaatcatgc
    68401 agcaccgacg tatggcactg ctggagttaa ttcaaaaaca tattcgtcaa cgcgacctga
    68461 tggggctggt agagcaaatg gcctgcttat taagtagtgg atacgctaat gacagacaaa
    68521 tcaaagggct gtttaattac atactgcaaa ctggcgatgc ggtacgtttt aacgatttta
    68581 tcgacggtgt ggccgaacgt tcaccgaaac acaaggagag tttaatgact attgcggaaa
    68641 gattgcggca ggaggggttt cagaaaggta tacgtaccaa cgccctaaat attgccaaaa
    68701 ccatgctgga tgccggagtc tctcttgagg acgttctgcg atttaccagc ctgacggttg
    68761 aagaccttgc tgaaataaat catcaataac gtaaaggatg gttttaatga cagatgcaga
    68821 aagagaatat cttattagtt acctgattgg ttatttattg gatcgacgcc tggaagccct
    68881 ggatatcgcc aaaagaatgc tgtaatccgg agtccatctt gcagacaccc tgcgttttac
    68941 cggggtgtca gaagaagagt tggctgcggc gagtcagtaa agttctgtct cgccatttca
    69001 aaagccacct acaccctctg cttcaacgcc accagcatgc gacaaaactc ggccggatgc
    69061 gaaataaacg gcgcatgggc cgctttggcg aagatgtatg attcgctgtg aggccaaagt
    69121 ttatccagca tcggcaccac tttgcgcggc accagaccgt cgagatagcc atacaatcgc
    69181 aaaaacggca tggacacgtt ttgcagcgac tgacggagat cgaccgtttt caggatttcc
    69241 agcccgccat taagcacgtc aacctccggc atcggtaacg ccagaacggt tttcttcagc
    69301 gcccgcgcat cctggcgggc cgtttcagta cccatggttt gtaacgccag gaaccgctcc
    69361 actgtacgct gaaaatcatc actgagttgc tgctgaaatc ccgccagcac gtccggtttt
    69421 atccccggcc actcgtcacg agcactaaaa caaggtgacg acgccacggt gaccagcgcc
    69481 tgaacacgct cgggatgggt taacgcaatc tggcttgcca ccagcccgcc cagactccag
    69541 cctaaccaaa tggctttatc aggtgcctgt cgcagcacgg cttcggccat atcagcaagt
    69601 gacatcgcac caaatccccg gctacgcccg aagccgggca ggtcaacaag gtgcagcgta
    69661 aaatgcgagc taagttcctc gtcaatgcaa cgccacactt cggcattcag tccccatccg
    69721 tgcaacagca caagatgaac attcccctga cctttggtct gccaccagat gttattcatc
    69781 cgttattgtt ctcttttgac ttacaaggat gaacatatgc taacagtacc gggattatgc
    69841 tggctatgcc gaatgccgct ggcgttaggt cattggggga tttgttcggt ctgctcacgc
    69901 gccgcccgta gcgataaaac gttatgccca caatgtggat tacccgctac acacgcccat
    69961 cttccctgcg gccgctgcct gcaaaaaccg ccgccctggc aaagactggt cacggttgcc
    70021 gactatgcgc cgccgttaag tccgcttatc caccagctta agttttcccg gcgcagcgaa
    70081 atcgccagcg ccctggcacg tctgttacta ttggaagtct tacacgctcg tcgcaccact
    70141 ggcttgcaac tgccggatcg catcatcagc gttccgttat ggcagcgacg tcactggcgt
    70201 cggggattta atcagagcga tttgctgtgt cagccgttat cacgcttgtt gcactgccga
    70261 tgggatagcg aagccgtcac acgaacacgg gccactgcga cccagcattt tctcagtgcc
    70321 cggctgcgca agcgcaacct gaaaaatgcc tttcgccttg aattgcctgt gcaaggtcgc
    70381 catatggtga tagtggatga tgtcgtcacc accggaagta ccgtcgcaga gattgcgcag
    70441 ctgcttttac gcaatggtgc ggcgactgtc caggtctggt gcctttgtcg aaccttgtag
    70501 agcctcgatg atgggcgtat tataaccaac taaaatagtc aactattagg ccattactat
    70561 gatccgtatt tccgatgctg cacaagcgca ctttgccaaa ctgctggcaa atcaggaaga
    70621 agggacacaa atccgcgtat ttgtgattaa ccctggcacg cctaacgctg aatgtggcgt
    70681 ttcttattgt ccgccggacg ctgtagaagc caccgacact gccctgaaat ttgacctgct
    70741 gaccgcgtat gttgatgagt taagcgctcc ttacctggaa gatgcagaga tcgactttgt
    70801 tacagaccag ttgggttccc agttaacgct gaaagcgcct aacgccaaaa tgcgtaaagt
    70861 cgcagacgat gcaccgctga tggagcgcgt ggagtatatg ctgcagtcgc agatcaaccc
    70921 acagcttgct ggtcacggtg gtcgcgtttc gctgatggaa atcaccgaag acggttacgc
    70981 cattctgcaa tttggcggcg gctgtaacgg ttgttccatg gtagatgtga cgctgaaaga
    71041 agggatcgag aagcagctgc tgaacgaatt cccggagctg aaaggtgtgc gcgatctcac
    71101 cgaacaccag cgcggcgagc actcctacta ctaagttatc ccctcttttg tagattgccc
    71161 gatgcgacgc taaagcgtca tttcgggcct acgttgaaca tgtgccagac gttggcgcag
    71221 cattgctttg gcaaatccct cccttcgttt cctcgcgata atatgaccaa cctctcataa
    71281 tttaaattta ccccgctctg gtgattctca aacgccggat gttacccgta tcattcacgt
    71341 gggtaccaaa catactcctg acatctgact acaataatta gttttggtgg gtatcagtcg
    71401 tggtgccgca atatctctgt tcccgattgg gataattaga gtttgtcgtc agaaaattga
    71461 cgttacccat aacaaatgaa aggccaggta aatcatgcca ttagtcattg ttgctatcgg
    71521 tgtaatcttg ttgttgctcc tgatgatccg cttcaaaatg aacggcttca tcgctctcgt
    71581 cctcgtggcg cttgctgttg gattaatgca aggaatgccg ctggataaag ttattggctc
    71641 catcaaagcc ggtgtcggcg ggacgctcgg tagccttgcc ctgatcatgg gttttggcgc
    71701 aatgctgggc aaaatgctgg cagactgtgg tggcgcacaa cgtatcgcca ccacgctgat
    71761 tgctaaattt ggtaaaaaac acatccagtg ggcggtggta ctgaccggtt ttaccgttgg
    71821 ttttgccctg ttctatgaag tgggtttcgt gctgatgctg ccgctggtgt ttaccatcgc
    71881 ggcttccgcg aatattccac tgctgtatgt tggtgtacca atggcggctg cactgtctgt
    71941 gacccacggc ttcctgccac cgcatccggg tccgactgcg attgccacca ttttcaatgc
    72001 cgatatgggt aaaactctgc tgtacggtac tattctggca atcccgaccg tgattctcgc
    72061 cggtccggtt tacgctcgcg tgctgaaagg tatcgataag ccgatcccgg aaggtctgta
    72121 cagcgcgaaa accttcagcg aagaagagat gccgagtttt ggcgtaagcg tctggacctc
    72181 tctggtcccg gttgtgctga tggcgatgcg tgcgattgcc gaaatgatcc tgccgaaagg
    72241 tcacgctttc ctgccggtag cggagttcct cggtgacccg gtaatggcta cgctgattgc
    72301 cgtactgatt gcgatgttca cctttggtct gaaccgtggt cgttcaatgg atcagattaa
    72361 cgacacgctg gtttcttcca tcaaaatcat tgcgatgatg ctgttgatta tcggtggtgg
    72421 cggtgcgttc aagcaggtgc tggtagacag cggcgtggac aaatacattg cttccatgat
    72481 gcacgaaacc aacatttctc cgctgctgat ggcctggtcg attgctgccg tactgcgtat
    72541 cgcgctgggt tccgcaaccg ttgcggcaat cactgcgggt ggtatcgcgg caccgctgat
    72601 tgcaacgacg ggtgttagcc cggagctgat ggttattgcg gttggttccg gtagtgtgat
    72661 tttctctcac gtgaacgatc cgggcttctg gctgttcaaa gagtacttta acctgactat
    72721 cggcgagacc atcaagtcct ggtcgatgct ggaaaccatt atctcggtgt gcggtctggt
    72781 aggctgtctg ctgctgaata tggtgatttg ataacaccga ctgccggatg catcgcatcc
    72841 ggcatcaacc gcaccctact acttcttcgc tgccgctctg cgccgtctgt ccaaatcctt
    72901 cagcaacttg ttcacgccat catcggcaaa catcgcctca agcgttgcag agagcttgcg
    72961 ccgccagttt ttatactggt aactggtgcc gggaatattt accggttccg ccatatccag
    73021 ccagtcttct ggctgtagcc ctaacagggc actgttactg tcagcgatat aacgttgcaa
    73081 accacgattc agcgtcggcg tcatcgacat caacgatgcc ttatgcccgg cacgttttgg
    73141 caggcaacca tatttatgca gagcatccag cagtccttgc ttcgccagtt cgcgatcctg
    73201 atacagaccg cgcagtacca cttcatccgg atacaacccc agcgttttgc ccagcgttag
    73261 atccccgctc tcccagtagc cgcgtagcgt aggcaggtcg tgtgtcgccg caaccgccat
    73321 cgactgctct gggtacgctt tcggtgagcg gaatgtcttc tcgtggtcgt tttcgaaata
    73381 gagcactttg taggagtaca caccgctgct gcgcagctta ccgacaatct ctaccggtac
    73441 ggtaccgaga tcttcaccaa tcaccatgca gcgatgacgt ttactttcga gtgccagaat
    73501 cgagagcaga tcatccaccg gatagtgtac atacgcgccc tgatccgccg tctcgccata
    73561 cggtatccac cacaaacgca gcatcgacat cacatggtca attcgtaatg cgccgcagtt
    73621 ttgcatattg gcacgcaaca gctcgataaa cggttcatag gcacgcgcgg tgatgatatg
    73681 cgggtccatt ggcggcaatc cccagttctg ccccaacggg ccgaggatat ccggcggtgc
    73741 gcctaccgac gctttcaggc aatacagttc acggtcgcac caggtttccg ccccaccttc
    73801 cgctacgcca accgccagat cacgatacaa gccaatcggc atttcatagc cctggcttat
    73861 ctcccagcag tcggcaaact ggctgtaagc cagccactgc aaccagagat aaaaatcgac
    73921 gtcatcacga tgttcttcgc agaactggcg cacttctggt gaatccacgt tctgatacat
    73981 ctctggccat gcaggccagc cccagcgcat ttcgtcctct ttcacttgct gggcatgtag
    74041 tgcgtcaaac gccgcctgcc agaacaggct gtcgccttgc tcggcaacaa actggcgaaa
    74101 cgcggtcatc tgttcatcat cacgttgcgc gaaacctttc cacgccattc gtaatgctgt
    74161 catttttagg gcggtaaccg tggagtaatc aacccagtcg gcatcccgcg cctgttgcag
    74221 cgtctgttgc gtggtcggca acttccacca ggcctgagcc tcttcgctaa gatggaaatc
    74281 ttcgacggcg ttaacgtcga tataaatcac attcagccaa cggcgagaag acgggctata
    74341 cgggctggca ctttccggat ttgccggata gagcgcatga atcgggttca gaccgatgaa
    74401 cgacccgcca cgttttgcca catcctcaag catcgctttg agatcgccaa aatccccaat
    74461 accccagttt ttttccgatc gcagcgtata aagctgaacg caggcacccc acagcttttg
    74521 tttattcagc aacgcctgcg gttcgtaaca gcgtttcggt gccacaatca cccggcaatg
    74581 cgcgcgctgg tcgtcctggg tgagtgtcag cgtgtgataa ccttccggca gcttcgtcgg
    74641 tagattgaac gctttgcccc ccgttacatg gcctttgtac tgcgttcctt cttcggtggt
    74701 cagcagccag ctatattcgc cgctgccctc caccaccatc ggcatttttt tgccgctggt
    74761 ataaaccatg acattcggga ctggcgttac cgccactttc gtggcggtac gttgatgcat
    74821 cgcgtcaagc aaacgccgtt tggtttcggc gctaatcgac tgcggtttac cgtgggcatt
    74881 gatgtaattg gggctaatcc ccgccgccag cgcggcatta tccagacgtt tgctttccat
    74941 cgagcttcct tagcgttttg cctgccagat acgagcctga taatcgcgaa tagagcgatc
    75001 cgagctaaac ataccgcagc gggcggtatt gaggatcgcc gcgcgggtcc aggcctcctg
    75061 gtcgcggtac agcacatcca cctgcttttg tgcctctaca taagcggcga aatctgccat
    75121 caccagatac ggatcgccgc cctgtttgcc gatactgtgc agcatctggt cgaaggcatg
    75181 cttatcgccg tcgctgtatt taccgctttc cagctctttc aatactgcgt ccagcacctt
    75241 atctttcttc cgccatttca ccgggtcgta gcctttggcc agaattgcct tcacttgttc
    75301 cacggtatga ccaaaaataa agatattttc ttcaccgact ttctcagcga tttcaacgtt
    75361 cgcaccatcc agcgtaccga cagtaagcgc accattgagc gccagtttca tattgccggt
    75421 accggaagct tctttacctg ccgtcgaaat ttgttcggag atatccgccg ccgggatcag
    75481 tttttccgcc gccgaaacac aataatccgg caagaacacc accttcaact tgtcgccaac
    75541 cagcggatcg ttgttgatca cgtcagccac tttgttgatc gcaaagataa tgtttttcgc
    75601 caggtagtag cctggtgctg ctttcgcgcc gaagaggaag acgcgcggta cgcgatcagc
    75661 ctgcgggttt tcacgaattt ctttgtacaa cgccagaata tgcagcagat tcaggtgctg
    75721 gcgtttgtac tcatgcagac gtttgatctg aatatcgaaa atcgcctgtg gattgatctc
    75781 aataccggta cgaactttca caaactctgc cagacggact ttattcgcct gcttgatctc
    75841 gcgatattgc tgacggaatt tcgcatcatc agcgaatttt tccagattga tcagctgatc
    75901 gagatcgtta gcccactctt ttttcagtga tttatccaac agagccgcca gtgccgggtt
    75961 gcactgtttg atccagcgac gtggggtaat accgttggtg acgttatgga atttgttcgg
    76021 ccatagctgg tgatattccg ggaacagatc tttcaccacc agatccgagt gcagcgccgc
    76081 aacaccgttc accgcgaaac cgccaaccac acacaggttc gccatatgca cttgtttgtc
    76141 gtgcaccacc gccagtttgg cccacacttt ttcatcgccc ggccaggtat tttccaccag
    76201 cgttttaaag cgtgtattaa tttcgttaat aatctgcatg tggcgcggca gtaagccttt
    76261 caccagtttc acatcccagc gttccagcgc ttctggcatc agggtatggt tggtgtaagc
    76321 gaatgttttg ctggtaatgg cccaggcgtc atcccagctc atctggtgct catcgatcag
    76381 cacgcgcagc agttctggaa tcgcgatagt tgggtgggta tcgttcagct gaataacttc
    76441 ataatccgcc agttcgtgca gtttacgccc cgccagatga tggcgacgca aaatatccgc
    76501 taccgaacag gcacactgga agtattgctg catcaggcgc agctttttac cggcagtatg
    76561 gttgtcgttt ggatagagaa ctttggtcag tttttccgcg ttgatgccct gctgttcggc
    76621 acgcaggaaa tcaccatcgt taaatttagt cagatcaaac ggatgcgcat gcgtcgcctg
    76681 ccacagacgc agtggctgcg ccacgccatt acgatagccg acaacgggga gatcccacgc
    76741 ttgaccagta atggtaaatt ccggctccca gcgtccgtct ttcgtcactt taccgccaat
    76801 tccaacctgc acatccagtg cttcattgtg gcggaaccac ggatagttac cacgatgcca
    76861 gtcatccggc gcttcaacct gtttgccatc gacaaatgac tggcggaaca agccatattg
    76921 ataattaagg ccgtagccag tagctgactg cccgacagtt gccattgagt cgaggaagca
    76981 cgccgccaga cgtcccagac caccgttacc cagcgccggg tcgatctctt cttccagcaa
    77041 gtcagtcagg ttgatgtcat aagccttcaa cgaatcctgt acatcctgat accagccgag
    77101 attcaacagg ttgttgcccg tcagacgacc aatcaaaaac tccattgaga tgtagttaac
    77161 atgtcgctga ttcgccaccg gtttggcaaa tggctgagca cgcagcattt cggccagtgc
    77221 ttcactcaca gccagccacc actggcgagg agtcatttca gccgcagaat ttaagccata
    77281 acgctgccac tgacgtgaaa gcgcttcctg aaattgctta tcgttaaaaa taggttgtga
    77341 cataggagtt ccacttttct tagattttca acacaacgtt atcgctagtt tgccaggctc
    77401 gatgttgacc ttcctcatcc tgcgggggat taggcaggga ggagttgcgg ggatgagcaa
    77461 ggaaatgtga tctcgaccac ttaaagctag tgcaaaccac aggattagca gcaaatcaat
    77521 gccatacagc gcagaaaatc tgtatctaag tgcaaaaaat ggccgttgcg tattttcaaa
    77581 aagcggaagg taactctata aattaagtaa aggagtgaaa cagtttcaca agtaa
//
LOCUS       DACBVI010000003        67766 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.003, whole genome shotgun sequence.
ACCESSION   DACBVI010000003 DACBVI010000000
VERSION     DACBVI010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.003"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            250..876
                     /locus_tag="IU325_01005"
     CDS             250..876
                     /locus_tag="IU325_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HAP5807613.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            897..2999
                     /locus_tag="IU325_01010"
     CDS             897..2999
                     /locus_tag="IU325_01010"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HAP5807614.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLNIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPGTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            3012..3650
                     /locus_tag="IU325_01015"
     CDS             3012..3650
                     /locus_tag="IU325_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HAP5807615.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDHADIACLTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIES
                     AIAAMDIAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3714..4382
                     /locus_tag="IU325_01020"
     CDS             3714..4382
                     /locus_tag="IU325_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAP5807616.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKKVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHYFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4379..5164
                     /locus_tag="IU325_01025"
     CDS             4379..5164
                     /locus_tag="IU325_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HAP5807617.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPQFWPVWLIIAGLLLVGMWFVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYIGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5168..5980
                     /locus_tag="IU325_01030"
     CDS             5168..5980
                     /locus_tag="IU325_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5807618.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGEDSTIVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(5992..7596)
                     /locus_tag="IU325_01035"
     CDS             complement(5992..7596)
                     /locus_tag="IU325_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HAP5807619.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKLMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKIDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVIFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPGWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDKNPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7722..8027)
                     /locus_tag="IU325_01040"
     CDS             complement(7722..8027)
                     /locus_tag="IU325_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HAP5807620.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLEPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8135..8211)
                     /locus_tag="IU325_01045"
     tRNA            complement(8135..8211)
                     /locus_tag="IU325_01045"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8175..8177),aa:Val,seq:gac)
     gene            complement(8216..8292)
                     /locus_tag="IU325_01050"
     tRNA            complement(8216..8292)
                     /locus_tag="IU325_01050"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8256..8258),aa:Val,seq:gac)
     gene            8601..9857
                     /locus_tag="IU325_01055"
     CDS             8601..9857
                     /locus_tag="IU325_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5807621.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGVTQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKANDTLLAGGTMNNLGGEDSDTIVENGGIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGNVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9898..11271)
                     /gene="mdtK"
                     /locus_tag="IU325_01060"
     CDS             complement(9898..11271)
                     /gene="mdtK"
                     /locus_tag="IU325_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HAP5807622.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRAAR"
     gene            11486..12127
                     /gene="ribE"
                     /locus_tag="IU325_01065"
     CDS             11486..12127
                     /gene="ribE"
                     /locus_tag="IU325_01065"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HAP5807623.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12167..13315)
                     /locus_tag="IU325_01070"
     CDS             complement(12167..13315)
                     /locus_tag="IU325_01070"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HAP5807624.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13606..14817)
                     /locus_tag="IU325_01075"
     CDS             complement(13606..14817)
                     /locus_tag="IU325_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HAP5807625.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCQNH
                     GNAEVAHSESH"
     gene            14930..15862
                     /locus_tag="IU325_01080"
     CDS             14930..15862
                     /locus_tag="IU325_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP5807626.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15859..16884)
                     /gene="purR"
                     /locus_tag="IU325_01085"
     CDS             complement(15859..16884)
                     /gene="purR"
                     /locus_tag="IU325_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HAP5807627.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17183..17272
                     /gene="ynhF"
                     /locus_tag="IU325_01090"
     CDS             17183..17272
                     /gene="ynhF"
                     /locus_tag="IU325_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HAP5807628.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17438..18607
                     /locus_tag="IU325_01095"
     CDS             17438..18607
                     /locus_tag="IU325_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5807629.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDISIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPEAVCVANS"
     gene            complement(18753..19334)
                     /gene="sodB"
                     /locus_tag="IU325_01100"
     CDS             complement(18753..19334)
                     /gene="sodB"
                     /locus_tag="IU325_01100"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HAP5807630.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19462..20289)
                     /locus_tag="IU325_01105"
     CDS             complement(19462..20289)
                     /locus_tag="IU325_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HAP5807631.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTIKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20624..20971
                     /locus_tag="IU325_01110"
     CDS             20624..20971
                     /locus_tag="IU325_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HAP5807632.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(21022..25638)
                     /locus_tag="IU325_01115"
     CDS             complement(21022..25638)
                     /locus_tag="IU325_01115"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP5807633.1"
                     /translation="MADNPNLSSPLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDQLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLQGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDIFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGISKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAASMDALQADEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFAASLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLVQRHTGSSEEIASYLE
                     NLLAVKRIFRTMISGQERLACMDDAARLRDALGVRLPESLPEIDLHRVSYPLRDLFLR
                     YLRAHALVTSEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSGEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEANQANRSALQQAIVTVLADGGAWFAQQISQRIRDKIGESVDPSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPK
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGSAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKDELLAPEIFQALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25731..26378)
                     /locus_tag="IU325_01120"
     CDS             complement(25731..26378)
                     /locus_tag="IU325_01120"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HAP5807634.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26481..26888)
                     /gene="gloA"
                     /locus_tag="IU325_01125"
     CDS             complement(26481..26888)
                     /gene="gloA"
                     /locus_tag="IU325_01125"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP5807635.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(26969..28066)
                     /gene="nemA"
                     /locus_tag="IU325_01130"
     CDS             complement(26969..28066)
                     /gene="nemA"
                     /locus_tag="IU325_01130"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HAP5807636.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(28103..28702)
                     /locus_tag="IU325_01135"
     CDS             complement(28103..28702)
                     /locus_tag="IU325_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP5807637.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIVLLSQALENGRDSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            28805..29044
                     /locus_tag="IU325_01140"
     CDS             28805..29044
                     /locus_tag="IU325_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HAP5807638.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            29093..29989
                     /locus_tag="IU325_01145"
     CDS             29093..29989
                     /locus_tag="IU325_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP5807639.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            30070..30591
                     /gene="sodC"
                     /locus_tag="IU325_01150"
     CDS             30070..30591
                     /gene="sodC"
                     /locus_tag="IU325_01150"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HAP5807640.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEVKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30592..32604)
                     /locus_tag="IU325_01155"
     CDS             complement(30592..32604)
                     /locus_tag="IU325_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP5807641.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMAA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPCATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHTRLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSGARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32604..33461)
                     /locus_tag="IU325_01160"
     CDS             complement(32604..33461)
                     /locus_tag="IU325_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAP5807642.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33464..33700)
                     /locus_tag="IU325_01165"
     CDS             complement(33464..33700)
                     /locus_tag="IU325_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HAP5807643.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            33901..34335
                     /gene="slyA"
                     /locus_tag="IU325_01170"
     CDS             33901..34335
                     /gene="slyA"
                     /locus_tag="IU325_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HAP5807644.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLIKLIAKLEHNIIELQAKG"
     gene            complement(34382..34849)
                     /gene="slyB"
                     /locus_tag="IU325_01175"
     CDS             complement(34382..34849)
                     /gene="slyB"
                     /locus_tag="IU325_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HAP5807645.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            35123..36232
                     /gene="anmK"
                     /locus_tag="IU325_01180"
     CDS             35123..36232
                     /gene="anmK"
                     /locus_tag="IU325_01180"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HAP5807646.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGGIFPANP"
     gene            36330..36659
                     /gene="mliC"
                     /locus_tag="IU325_01185"
     CDS             36330..36659
                     /gene="mliC"
                     /locus_tag="IU325_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HAP5807647.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36718..37374
                     /gene="pdxH"
                     /locus_tag="IU325_01190"
     CDS             36718..37374
                     /gene="pdxH"
                     /locus_tag="IU325_01190"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HAP5807648.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37503..38777
                     /gene="tyrS"
                     /locus_tag="IU325_01195"
     CDS             37503..38777
                     /gene="tyrS"
                     /locus_tag="IU325_01195"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP5807649.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38839..39699
                     /gene="pdxY"
                     /locus_tag="IU325_01200"
     CDS             38839..39699
                     /gene="pdxY"
                     /locus_tag="IU325_01200"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HAP5807650.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARKLIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(39743..40348)
                     /gene="gstA"
                     /locus_tag="IU325_01205"
     CDS             complement(39743..40348)
                     /gene="gstA"
                     /locus_tag="IU325_01205"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HAP5807651.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNDALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40454..41956)
                     /gene="dtpA"
                     /locus_tag="IU325_01210"
     CDS             complement(40454..41956)
                     /gene="dtpA"
                     /locus_tag="IU325_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HAP5807652.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(42567..43202)
                     /gene="nth"
                     /locus_tag="IU325_01215"
     CDS             complement(42567..43202)
                     /gene="nth"
                     /locus_tag="IU325_01215"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP5807653.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIRTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFALGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(43202..43897)
                     /locus_tag="IU325_01220"
     CDS             complement(43202..43897)
                     /locus_tag="IU325_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit E"
                     /protein_id="HAP5807654.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(43901..44521)
                     /gene="rsxG"
                     /locus_tag="IU325_01225"
     CDS             complement(43901..44521)
                     /gene="rsxG"
                     /locus_tag="IU325_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HAP5807655.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLETTAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPSQLSQLPACGE"
     gene            complement(44525..45583)
                     /gene="rsxD"
                     /locus_tag="IU325_01230"
     CDS             complement(44525..45583)
                     /gene="rsxD"
                     /locus_tag="IU325_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HAP5807656.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTPSGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLASPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(45584..47710)
                     /gene="rsxC"
                     /locus_tag="IU325_01235"
     CDS             complement(45584..47710)
                     /gene="rsxC"
                     /locus_tag="IU325_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HAP5807657.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGSEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWDDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIHQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAVREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAKPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEKVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQKANAEPEEQVDP
                     RKAAVAAAIARVQAKKAAQQKVVNED"
     gene            complement(47703..48281)
                     /gene="rsxB"
                     /locus_tag="IU325_01240"
     CDS             complement(47703..48281)
                     /gene="rsxB"
                     /locus_tag="IU325_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HAP5807658.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPTRMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(48281..48862)
                     /gene="rsxA"
                     /locus_tag="IU325_01245"
     CDS             complement(48281..48862)
                     /gene="rsxA"
                     /locus_tag="IU325_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HAP5807659.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(48939..49379)
                     /locus_tag="IU325_01250"
     CDS             complement(48939..49379)
                     /locus_tag="IU325_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 family protein"
                     /protein_id="HAP5807660.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGEQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(49465..49680)
                     /gene="ydgT"
                     /locus_tag="IU325_01255"
     CDS             complement(49465..49680)
                     /gene="ydgT"
                     /locus_tag="IU325_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HAP5807661.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(49953..50078)
                     /gene="blr"
                     /locus_tag="IU325_01260"
     CDS             complement(49953..50078)
                     /gene="blr"
                     /locus_tag="IU325_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HAP5807662.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            50321..51361
                     /locus_tag="IU325_01265"
     CDS             50321..51361
                     /locus_tag="IU325_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP5807663.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELEALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILQGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGQRLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(51397..52398)
                     /locus_tag="IU325_01270"
     CDS             complement(51397..52398)
                     /locus_tag="IU325_01270"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP5807664.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVSFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(52502..53674)
                     /gene="malY"
                     /locus_tag="IU325_01275"
     CDS             complement(52502..53674)
                     /gene="malY"
                     /locus_tag="IU325_01275"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HAP5807665.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKRADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWAEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWIDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(53684..55276)
                     /gene="malX"
                     /locus_tag="IU325_01280"
     CDS             complement(53684..55276)
                     /gene="malX"
                     /locus_tag="IU325_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HAP5807666.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKTNNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGVPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            55451..56479
                     /gene="malI"
                     /locus_tag="IU325_01285"
     CDS             55451..56479
                     /gene="malI"
                     /locus_tag="IU325_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mal regulon transcriptional regulator MalI"
                     /protein_id="HAP5807667.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEEAHSRNLII
                     PARLIAPK"
     gene            56591..57358
                     /gene="hdhA"
                     /locus_tag="IU325_01290"
     CDS             56591..57358
                     /gene="hdhA"
                     /locus_tag="IU325_01290"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HAP5807668.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFAWRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            57579..58169
                     /locus_tag="IU325_01295"
     CDS             57579..58169
                     /locus_tag="IU325_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP5807669.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            58559..60370
                     /gene="uidA"
                     /locus_tag="IU325_01300"
     CDS             58559..60370
                     /gene="uidA"
                     /locus_tag="IU325_01300"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP5807670.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQLWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDFYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            60367..61740
                     /gene="uidB"
                     /locus_tag="IU325_01305"
     CDS             60367..61740
                     /gene="uidB"
                     /locus_tag="IU325_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HAP5807671.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKMQQQ
                     LISDITN"
     gene            61779..63044
                     /gene="uidC"
                     /locus_tag="IU325_01310"
     CDS             61779..63044
                     /gene="uidC"
                     /locus_tag="IU325_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HAP5807672.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSALGDRLNYWDVKMSIQYDFMLK"
     gene            complement(63089..64597)
                     /locus_tag="IU325_01315"
     CDS             complement(63089..64597)
                     /locus_tag="IU325_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HAP5807673.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYRGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(64698..65873)
                     /gene="manA"
                     /locus_tag="IU325_01320"
     CDS             complement(64698..65873)
                     /gene="manA"
                     /locus_tag="IU325_01320"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAP5807674.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPLDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            66072..67718
                     /locus_tag="IU325_01325"
     CDS             66072..67718
                     /locus_tag="IU325_01325"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HAP5807675.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
BASE COUNT        17050 a        16700 c        17649 g        16367 t
ORIGIN      
        1 agttcactta taatttcttg ctttgtaatt ttaattgata acgatcaatg ttaagggctc
       61 agaataatca cattattttt tctcctaacc ataaaggatt agtttatcgg cgttaaatct
      121 tttctcatac actatcgggt tagggaaaat atctggtgtt ggggacagag tcagcagcgt
      181 attagttgaa tgcttaatat caggctgctg aattgtcagc actgtgtttg cgaagaggat
      241 gcgaaataaa tgaacccggt tgatcgtcca ctattagata ttggcttaac gcgattagaa
      301 ttcttgcgta tatcaggaaa aggccttgct ggtctaacaa ttgcccctgc gttactgtca
      361 cttttgggtt gcaaacagga agatattgat agcggcacag tagggttgat aaatacaccc
      421 aaaggggtgc tggtgaccca gcgagcgcga tgcacgggtt gccaccgctg tgaaatctct
      481 tgtaccaact tcaacgatgg ctcagtaggg acattcttct cccgtatcaa aatccatcgc
      541 aattatttct ttggcgacaa cggggttggc tctggcggcg gcctgtatgg cgatctcaac
      601 tataccgcgg acacctgccg acaatgcaaa gagccgcaat gcatgaacgt ctgcccgatt
      661 ggtgcgatta cctggcagca gaaagaaggc tgtattaccg tcgatcataa acgctgtatt
      721 ggctgtagcg cctgtactac ggcatgcccg tggatgatgg ccaccgtaaa taccgaaagt
      781 aaaaaatcct cgaaatgtgt gttatgcggt gaatgcgcaa acgcctgccc aacaggggcg
      841 ttaaaaatta tcgagtggaa agatattact gtgtgaatct tgcaaaggaa aaaatcatgg
      901 ctaacggttg gacaggtaat atattaagag tcaatctcac gacaggaaat attaccctcg
      961 aagattccag taagtttaaa agttttgttg gtggtatggg cttcggctac aaaattatgt
     1021 atgacgaagt accgccaggc acgaaacctt tcgatgaagc gaataaatta gtctttgcta
     1081 ccggcccatt aactggatct ggtgccccct gtagttctcg cgtaaatatc acctcacttt
     1141 ctacttttac caaaggaaat ttagtcgtcg atgcccatat ggggggcttt tttgcagcgc
     1201 aaatgaaatt cgctggatac gacgtcatta ttatcgaagg gaaagcgaaa tcaccggtat
     1261 ggctgaatat taaagatgac aaagttagcc tggaaaaagc cgatttctta tggggaaaag
     1321 gtacgcgcgc aactacggaa gagatttgtc gcttgaccag ccctgaaacc tgtgtggcgg
     1381 ctattggtca ggctggggaa aaccttgttc ctctctctgg catgttgaat agccgtaacc
     1441 acagcggtgg tgcgggaact ggcgcaataa tgggttcgaa aaacctgaaa gcgattgcgg
     1501 ttgaagggac gaaaggggtc aacattgccg atcgtcagga gatgaagcgt ctcaacgatt
     1561 acatgatgac tgaacttatc ggtgcgaata acaaccatgt cgtgccaagt acgccgcaat
     1621 cgtgggcaga gtattcagat cccaagtcac gttggacagc acgtaaaggg ctgttttggg
     1681 gcgcggctga aggtggtccg attgaaacgg gggaaattcc gccaggcaat cagaacacgg
     1741 tcggctttcg tacctataaa tccgtttttg acttaggtcc ggcggcagag aaatacacag
     1801 taaaaatgag cggctgccac tcttgcccga tccgttgtat gacccaaatg aatattcctc
     1861 gggtgaaaga gtttggcgtg cctggcaccg gtggtaacac ttgtgtagca aactttgtcc
     1921 ataccaccat cttcccaaac gggccgaaag attttgaaga taaagacgat ggtcgtgtga
     1981 ttggtaacct ggttggtctg aatctattcg atgactacgg cctatggtgt aactatgggc
     2041 agctgcatcg cgactttact tattgttaca gcaaaggggt gttcaagcgt gttctaccag
     2101 ctgaagagta tgcggaaatt cgctgggatc aactggaagc gggtgacgtt aacttcatta
     2161 aagattttta ctaccgtctg gcgcatcgtg tgggtgagct gagtcacctg gctgatggtt
     2221 catatgccat cgcagaacgc tggaatttgg gtgaagagta ctggggctac gcgaaaaata
     2281 aactctggtc gccgtttggc tatccagttc accatgccaa tgaagcgtca gcgcaagtcg
     2341 gttccattgt taactgtatg ttcaaccgtg actgcatgac gcatacccat atcaatttta
     2401 ttggttccgg tttgccattg aaactgcaac gtgaggtggc gaaagagctt tttggttctg
     2461 aagatgctta cgatgaaacc aaaaactaca cgccaatcaa tgacgcaaaa attaaatatg
     2521 ctaagtggtc gctgttgcgg gtctgtttgc ataacgccgt cacactgtgc aactgggtct
     2581 ggccaatgac cgtttcgccg ctgaaaagcc gtaattatcg gggcgatctg gcgcttgaag
     2641 ccaaattctt caaagcgatc actggcgaag agatgactca ggaaaaatta gatttagctg
     2701 cagagcgtat ctttacgttg catcgtgcct acacggtaaa actgatgcaa accaaagata
     2761 tgcgtaatga acacgatctt atctgttcct gggtattcga caaggatccg cagatcccgg
     2821 tctttactga aggtactgac aaaatggacc gtgacgatat gcatgcttcg ctcacgatgt
     2881 tctacaaaga aatgggctgg gacccacagc ttggttgtcc gacccgtgaa acattgcagc
     2941 gtctggggct ggaagatatt gccgccgatc tggcagcaca caatctactg cctgcgtaag
     3001 gaatgggtga gatgaaccat caggatgaat tgccgttagc gaaagtcagc gaagttgatg
     3061 aggcgaagcg gcaatggttg caggggatgc gtcaccccgt tgatacggta accgaaccag
     3121 aacctgctga aatcctggca gagttcattc gtcagcattc tgcggctggg cagctggtgg
     3181 cgcgcgccgt gtttctttcg ccaccctatt cggttgcaga agaggaactg tcagttttgc
     3241 tggaaagcat taagcagaac ggagaccacg cggatatcgc ttgtttgacg ggaagccagg
     3301 atgattacta ttactcaact caggccatga gtgaaaacta cgcggcgatg tctttgcaag
     3361 ttgtggagca ggacatttgt cgcgccattg cacacgccgt tcgttttgag tgccagacgt
     3421 atcctcgtcc ttacaaagtc gccatgctga tgcaagcgcc atattacttc caggaagcgc
     3481 agatcgaatc cgcgatcgcc gcgatggata ttgcacctga atatgctgat atacggcagg
     3541 tggagtcatc gacggcggtg ttgtacttgt ttagcgagcg atttatgacc tatggcaagg
     3601 cgtatggctt atgcgaatgg ttcgaggtag agcagtttca gaacccctga aatttcaggg
     3661 agatgtcatg gatagggtgg acagttgcag ccactgcatt aggggataat cagatgtcct
     3721 tcactcgacg caaatttgtt ctggggatgg gaacagtaat cttttttact ggttctgcgt
     3781 cttctctgtt agcgaacacg aggcaagaaa agaaagttcg gtatgccatg attcatgacg
     3841 agtcacggtg caatggctgt aatatttgcg cccgcgcatg ccgtaaaact aaccacgtcc
     3901 ccgctcaggg aagccgttta tcgatagcac acattccagt caccgataac gacaacgaaa
     3961 cgcagtatca ctattttcgc cagtcatgcc agcactgtga agatgctcct tgcatcgacg
     4021 tatgcccgac aggggcgtcg tggcgtgatg agcaggggat cgtgcgggta gaaaaatcgc
     4081 agtgcatcgg ctgcagttat tgcattggcg cttgtcctta ccaggttcgc taccttaacc
     4141 ctgtcactaa agtggcagat aaatgtgatt tctgcgctga gtcccgactg gcaaaaggct
     4201 ttccgcccat ttgcgtcagc gcctgtccgg aacacgcatt gatctttggt cgcgaagata
     4261 gcccagaaat tcaggcgtgg ttgcaggaaa ataaatacta tcaatatcag ctacctggtg
     4321 ccgggaaacc gcacctgtat cgccggttcg gtcaacattt gattaaaaag gaaaatgtat
     4381 gaacccgtcg caacatgctg aacagtttca gagccagttg gcgaactatg tgccacagtt
     4441 cactccccaa ttttggccgg tgtggttgat cattgccgga ctattgctgg tagggatgtg
     4501 gtttgtgctg gggctgcatg ccttgcttcg tgctcgtggc gtgaagaaat cagccaccga
     4561 ccatggtgag aaggtttatc tttacagcaa agcggtcaga ttatggcact ggtcgaatgc
     4621 gttactcttt gtattgttgc tggcaagtgg gctgataaat cactttgcgc tggtgggcgc
     4681 aactgcggtt aaaagtctgg ttgcggtgca tgaagtttgc ggatttttgt tactggcatg
     4741 ttggctcggc tttgttctga tcaatgccgt tgggggtaat ggtcaccatt atcgcattcg
     4801 tcgtcagggg tggctggagc gagcggcaaa acaaacgcga ttctatttgt ttggcattat
     4861 gcagggggaa gaacatcctt tcccggcaac aacacagtct aaatttaatc ccttacagca
     4921 ggttgcctat attggtgtca tgtatggatt gctgccgttg ttactattga cggggctgct
     4981 gtgtctctat ccgcaagccg tgggagatgt gtttcctggc gtaagatact ggttattgca
     5041 ggcgcatttt gctctggcat ttataagcct cttttttatc ttcggtcatc tttatctttg
     5101 caccacgggg cgtacgccac acgaaacctt taaaagcatg gtcgatggct atcaccggca
     5161 ctaacgaatg atcattacac gagcagacct gcgagaatgg cgtatcggag cggtgatgta
     5221 tcgctggttc ctgcgccatt tcccacgcgg cggtagttat gccgatattc atcatgcact
     5281 gattgaggaa ggatatacgg actgggcgga gagtctggtt gagtatgcgt ggaaaaaatg
     5341 gctggcggat gaaaacttcg cgcatcagga agtttcctct atgcaaaaat tggccacaga
     5401 tcccggagag agaccatttt gcagtcagtt tgcccgcagc gacgatcatg ctcgcatcgg
     5461 ctgttgtgag gataatgcca gaatcgcaac cgctgggtat gccgcgcaaa ttgccagtat
     5521 gggttatagc gtgcggattg gaagtgtcgg ctttaacagt cacattggca gctccggtga
     5581 acgcgcacgg gttgctgtaa ccggtaattc ttcgcggata agcagcgctg gagatagtag
     5641 tcggattgcg aataccggaa tgcgagtacg cgtttgtaca ttgggtgaac ggtgtcatgt
     5701 cgccagtaat ggtgacctgg tgcaaatcgc cagctttggc gcaaacgcca gaattgccaa
     5761 cagcggcgac aatgtacaca tcattgccag cggtgaagat tcaacaatag tgagcacggg
     5821 cgtggtcgac tcaattattc tcgggccagg gggaagcgcc gcactggcat atcatgacgg
     5881 cgaaagagtt cgcttcgccg tcgccattga aggtgaaaac aatattcgag ccggagtccg
     5941 ctatcgtctt aatgaacagc atcagtttgt tgagtgctga tagctttacc tttaatcaaa
     6001 cgaaagacgc ctacgtgcac gggaggcagg ttttacgaca gcccgagcca tcgcctcacc
     6061 aatttctgca catgccgtaa gtccctgaac gaaaggctgg tcgtgcatta accagggcaa
     6121 cgcgccgaac gctacgcgcc cacgaatatc ttcgggttgc tgtaacgtat aatcttcgcc
     6181 aacatcaggg tttttatccc ctgttttctg taattgttcg cgtagtccag ggaaaggaat
     6241 atctttcact ttaagcggac gctgcccacg ggcatcaata aaaacgtcaa acgagtagct
     6301 gttgtcttcc gttttcagga cggtgcgcga ctcattaatt tccatttcgt agtcttcacc
     6361 gagggcgaga atatgaatga ttcccgcttc acgtaaggcc agtagtcgac gaatagactc
     6421 tgaagggatt gccgcataat tatcgatgaa aacccgggca aggcctttac tgaaccgttt
     6481 atggtcgtgt tcattcagat gtggaacaat ttcctgtact gcttcatgca ggcgcagaat
     6541 gacataacgc cagggaacag tatgtttttc tcgtttattg cgttcgactt cctggagatt
     6601 tttttctgcc cagtcaaatg ggtcgcgttg tttgcgctcg gcaaaccagg cttgagcaaa
     6661 ggaatcgaca ttcaggctct ctaaggctat gcgttggctc cagcctggat cagcaaactt
     6721 gatttcctct actatcaatc taaatacccg atccaaaagg ccatattcgc ctttttgaat
     6781 ctcagcattt aatgcttgat cagtgacgat gtgtaagggc tcgtagggaa tagggcaata
     6841 gaaatcggct tcgggtaaaa tacccgtgcg cgacattaac gtaatattta gcttttcact
     6901 tgcgttatcg cggtgaaaaa tgacgtgttg tttatcatct tcaatgaacg aaccatgctg
     6961 aatagccact gccattgccg catccagtcc gctcaaggat gttcccataa tacccacgtt
     7021 acacgcatcg atctttgctt ccatcaggcc tgaccacggg ctgggaaagt acgttctggt
     7081 tgcttcttct tcatcaggcc agacgtgacc cgtggcaatc accgctaaat caaacgtctc
     7141 gctgggtaaa tcctgatttg tagcgagcat gacgccagca tttgtaattt gcagatcggt
     7201 aacctggcat gattcataaa cagccaccgc aaatttttgc tgtcgtgcct ggtctactaa
     7261 tcgtaaaaat tgatcgcgga aatattcgcc cagcagaatt cgcggtaaaa actgacgatc
     7321 gtgcaaggtt tcttttttaa cgccataacg ctggagatgg ctggcttctt gcttttgtag
     7381 ccattcgaga tacgtacaat aaatcggcgg tatttcaata ctggcaatat ttgccagcat
     7441 cagttttgag ttttcctcat cactgtatgg catcccgaca ccggcttcgt cagcctgctc
     7501 gaagatagaa atagaaagtg gagtttgttg ctgtagaagc gagaataagg tgtagatccc
     7561 cgtaggcccg gcacccacaa tagcaatttt tttcattgtt acttacctgt acgcattctt
     7621 tcgaatttag tgttatcaga taagtttggt tgaaattttt agaatttgag aaatttcaga
     7681 cagggatgaa aagggcgcta accgcccttg gttaaaatct gtcaggcgag ttttgcctga
     7741 ttgatttgac tcagtggctc attgacatcg aaaactttgg cgacaacttc ctcaacgccg
     7801 agatttttca gtcgggcagt atgtttttcc agataggcaa gggcgctttt ttcatcagtg
     7861 aaaaggtaga tcccaccggc ttcgtggttc ttttcacttt ctgtccatac cttccacaga
     7921 aaaccaggtt cctgattaat cgactcagca agcggttcaa gctgctcagc cattgcgtcg
     7981 ccaaatgggc cattaaaagc aaaatgaagt tgtaacaaag ttgccatagc gattctcctt
     8041 ctgataacgc atacgaagac agtgtaaatc ttttaatgta ttggatacag agaagaaaca
     8101 gagggaaaaa ggagaagacc agattaagaa aatctggtgc gtccgagtgg actcgaacca
     8161 ccgaccccca ccatgtcaag gtggtgctct aaccaactga gctacggacg caggatggtg
     8221 cgttcaattg gactcgaacc aacgaccccc accatgtcaa ggtggtgctc taaccaactg
     8281 agctatgaac gcaacgttgt aggtgacaac ggggacgaat attagcggca gagtgggaag
     8341 gtggcaagag gcaaaacgta attttctgcg ctatttcgac cgtttgcaga gcttttaagc
     8401 aaattggcta tattttgttg atttgcaagg gcgattttta ttcaggatcg catttacatc
     8461 tgacacaacc caataatctg tgtatcagag tttaaacaga gttgtttgcc accatctcaa
     8521 aatctgtcgt ggataaatcc tccgtagcct ttacacttag gtagcaataa aggcacacag
     8581 atgcagcaaa ggaataaatc atgggatctg atgcgaaaaa cttgatgagc gacgggaacg
     8641 tgcaaattgt taagaccggc gaggtcattg gcgtgacgca acttactgaa ggtgagttaa
     8701 ttgttgaagc tggcggaaga gccgaaaata ccgtggtcac gggggctggc tggttgaaag
     8761 tggcaaccgg tgggatcgcc aaatgcacac agtacggcaa caatggcacg ctatcggtca
     8821 gcgacggtgc cattgccaca gatattgttc agtccgaggg aggcgcaatt agtctctcta
     8881 cgctcgctac ggttaatggc cgccatcccg aaggtgaatt cagcgttgat aaaggttatg
     8941 cctgcggttt gttgctggaa aatggcggta acctgcgtgt actggaaggc catcgcgcgg
     9001 aaaaaattat tctcgatcaa gagggtggcc tgttggtcaa tgggacaacc tcagcggtcg
     9061 tggtagatga aggtggtgaa ttgttggtgt atccaggtgg ggaagccagc aattgtgaga
     9121 ttaatcaggg cggcgttttt atgctggcgg ggaaagccaa tgatacgttg cttgctggtg
     9181 gcaccatgaa taatctcggt ggtgaagact ctgacactat tgttgagaat ggaggcatct
     9241 atcgtctggg tacggatggt cttcagctct acagttccgg taagacgcaa aacctgtccg
     9301 ttaatgtggg ggggcgggct gaagtgcatg ccggtacgct ggaaaatgcg gtaatacaag
     9361 gtgggacagt catcctgttg tcacccacca gcgcggacga aaattttgtc gtagaggaag
     9421 atcgcgcacc ggttgaactg accggtaatg ttgcattact ggacggcgct tcaatgatta
     9481 ttggctatgg cgcagatctg caacaatcaa cgattactgt acagcagggc ggtgtattga
     9541 ttctcgacgg cagtacggta aaaggtgaca gtgtcacttt cagtgttggt aacatcaatc
     9601 taaatggcgg aaaactgtgg ctaatcactg gtgcggcaac gcatgtgcaa ctgaaagtga
     9661 aacgcctgcg cggagaggga gcgatttgcc tgcaaaccag tgcgaaagaa atttcacctg
     9721 acttcatcaa tgtgaaaggg gaagttactg gtgatataca cgttgagata acagatgcca
     9781 gtcggcaaac tctgtgtaac gcactgaaac tacagccaga cgaagacggg attggcgcaa
     9841 cgctccagcc tgcgtaaaaa cacaggcgca acccgtgaag gttgcgcctt gtctttacta
     9901 gcgagctgct cgttgcagaa tgatggctga cggcagacgt tgcaggaacc gcatacgcag
     9961 catcatcata atggctgccg acgtcaggcc aataataaag cctatccaga agcctgctgg
    10021 ccccataggt tcaacgacca gatcggtcag tgccagaata tagccgcttg gcaagcccag
    10081 cacccagtaa gccgtaaagg tgatatagaa aatggaacgc gtatctttat aaccacgcaa
    10141 aatcccactg ccaatcacct ggatagagtc agaaatctga tataccgccg ccagcaacat
    10201 caaatgcgca gccagcgtta caacctcggg attgtcgttg tacaacaggg cgatttgctc
    10261 ccgcagtgaa accgtgaaaa tggccgtcag ggttgccata cagacaccca ccataagccc
    10321 ggtccgcgca gcggtttgcg catccagcgt tgagccctga cccagacgat aacctacgcg
    10381 gatagttacc gctgccgcca gcgacattgg aagcacaaac atcagtgaac taaagttcag
    10441 ggcaatctgg tgtcctgcga catcaacaat accgagcgga gacactaaca gagccacgac
    10501 ggcaaacagc gttacttcaa agaacagcgc cagcgcaatt ggcaaaccga gttgaatcag
    10561 tcgtttcata accgcaggat cgggcttcgc agtgcctttt tcgttacgaa tatcgcgcat
    10621 cgagcgggcg cgtttaatgt aagaaaccat ggcaaggaac atgacccaat acaccgcagc
    10681 agtagccacg ccacaaccaa cgccaccgag ctcaggcata ccgaaatgac cgtaaataaa
    10741 gatatagttc accggaatgt tcaccagcag gccgataaag cccatcacca tacccggctt
    10801 ggtttttgcc agaccttcac actggttacg ggcaacctgg aagaaaagat agcccggcgc
    10861 gccccatagc aacgcacgca gataacccac ggctttgtcc gccagagccg gatcgatgtt
    10921 ttccatggag cggataatgt aacctgcatt ccacagcacc agcataatga gaacggaaac
    10981 aaaacccgcc agccagaaac cttgtcgcac ctgatgcgca atgcgctcac gtcgaccgga
    11041 accatttaat tgcgcgataa ccggcgttaa tgccagcagc agtccgtgac caaagaggat
    11101 cgccggaagc cagatagaag taccgatagc gaccgccgcc atgtcggtgg cactatagcc
    11161 gcccgccatc acggtatcga caaaacccat cgcagtttgg gcgatttgcg cgagaatcac
    11221 cgggattgct aatgctaata acagacgcgc ttcactgata tacttctgca cgtgaacacc
    11281 ttttatttgt agttatatga gagactaaaa agccgcctga atgggcagca aaaagaagaa
    11341 gcaggggaaa ttcagtctat tgtagcgagg atttattaat tctccagtga aaaaatagcc
    11401 attcttaccc cttcactggt aagggcgttt gccacctgct attgtgctga acagaatgtc
    11461 ttaactgatt tcaggagttg taagtatgtt tacggggatt gtacagggca ccgcaaaact
    11521 ggtgtcgatt gacgagaaac caaattttcg cacacatgtg gtggagttac ccgaccacat
    11581 gctggacggc ctggaaaccg gtgcgtccgt ggcgcataac ggttgctgcc tgaccgtgac
    11641 ggaaattaac ggcaaccatg tcagttttga cctgatgaaa gaaacgttac gcattaccaa
    11701 tcttggcgat ttaaaagtgg gggattgggt aaacgttgag cgtgcggcga aattcagtga
    11761 tgaaattggc ggacacttaa tgtcaggtca tattatgacc accgcggaag tggcgaaaat
    11821 attaacctcg gaaaataatc gccagatctg gtttaaagtc caggatagtc agttgatgaa
    11881 atatattctg tacaaaggat ttattggcat cgacggtatt agcctgaccg tcggcgaagt
    11941 cacgccaacg cgtttttgcg tccatttaat tccggaaacc ctggaacgca cgactcttgg
    12001 gaagaaaaaa ctcggcgcac gcgtcaacat tgaaattgat ccacaaactc aggcagtggt
    12061 agataccgta gaacgtgtgc tggcggcacg agaaaatgcc atgaatcaac caggtacaga
    12121 agcctgatct gaaatcgccc ccggagaggg ggcgatagaa tagcctttag cgagccactc
    12181 gaaggccgtt ttcaacaccg cgtgagaaca ccacctgcca gagctgaata tcacgggcgc
    12241 ggaaagcacc cgcacaggca ttcagataat aggtaaacat ccgtttaaag cgttcactgt
    12301 agttatccgc aatttctggc catgcggcga ggaatcgttc ataccacgcc atcaacgtag
    12361 tatcgtaatc agcaccgaag ttatgccagt cttccatcac aaagtggggt tcgctggact
    12421 gagcaatctg gcgtacagag ggcaggcaac cgttcggaaa aatatattta ttaatccagg
    12481 gatcaacatt cagatcggtt tttttcgaac cgatagtatg gagcaggaat atgccttccg
    12541 gtttcaaatt acgatccacc accgcaaaat aggtatcgta atttttcggt ccgacgtgct
    12601 cgaacatccc cacagaaaca atacgatcaa actggtcgtt caggtcacga taatcttgca
    12661 gcaaaatggt gacatccagg ccttcacagc gttcctgagc cattttttgc tgttcggcag
    12721 aaatggtgac gccaaccacg cttacgtcat aattagatgc catgtagtgc gccagtccgc
    12781 cccagccgca gccaatatcc agtacgcgca tccctggttt taactgcaat ttttcacaaa
    12841 tcattttgag cttcgcctgc tgggcagatt ccagattatc ggcatctttc cagtaagcgc
    12901 aggaatattg catgaaggga tcaagcatgc ggctgaacaa gtcattaccc aaatcgtaat
    12961 gctctttgcc gactatccag gcacgttttt tactctgaag attgaagaga cgagcgccgg
    13021 caatacgcag cgtgtctttg aaatgatggg ggagttggtt ctcgagacct gcgcgtaaga
    13081 ctttgctaaa aaacatatcc agtcggtcac attcccacca gccatccata taactttcgc
    13141 ctaaccccaa agagccttcc tgcagaacgc gtttaaaaaa atcggggttt ttcacacgaa
    13201 tatccgccgg ggcagaaccg ttaatggcta taccggcacg gctaagtaat tcgttggcga
    13261 tacggtacca gttgtcatcc ggtacactga cttcttctat acacgatgaa ctcatagttt
    13321 ctccatcact ggtcggtgat cagaacctta aaacagcgta gacgcttttt gggctttgtg
    13381 agaaatctcg cagaaaaaac cgcgagtcta taatccgacg tagaacagag ggaaaggagg
    13441 aacccttgcc gaaatgacca tccatagcga gtcgggaacg atcctgtccc cgtaaaaaca
    13501 taatatttat cgtagcttta acaactcgaa attagtatag gccgctaatt tcaatcatac
    13561 aacaaaattt tgttagcagc ctaagtataa gtatatcgat atagatcagt gtgattcgct
    13621 atgagcgact tcggcattgc catgattctg gcagtcaact tcttcacaac gttgcatcat
    13681 gtaacccaga gcaaccagca ctactgttga taacatcacg ctggtggtgg tgagcaatgg
    13741 cgtgctgata ctgataagcc aggaaactac cagacttgcg aggaagcaca gacccagttg
    13801 tagagtgttt tgcaacgctg cggcgcgacc agttgcatgt gggaagggac gcagcgcctg
    13861 ggcgacaaca atcgggtaga tcgcgccatt ggcaatcgcc atcacacaga atgggatcag
    13921 gatttcgact agcgacacat ggctaatgaa gcccgcagcc caggtcgcaa tgacgctgac
    13981 agcaaacagt accagcaacc acggtaataa ctgcttgcct tgccatttct gcagtgcggc
    14041 gcgacagcca taaccaccaa tcagaaacgc gatagtttgc gggacataac ttaaaccaat
    14101 aactgccggg ctgtagccca tttcactaag gatgaacggt gagccggtca gccatgcaaa
    14161 aaaactggct gaacaggccg catatatcag cacgttgccg cgataggttt tagaacgtag
    14221 caggtcggta aaggtcagac catcctgact attgttacgg gccttcgtcg tgggtttgag
    14281 ccagaaaata ggcagaatca gcaccacggt aatggcaaac agggtggcga aaatcgcctg
    14341 ccaggaaaaa tggaccagca gccagcttcc taacagagga gccagtgccg gagatagacc
    14401 caccagcggc atgatggtcg caaaaatacg gttaactttc tgtgaaggat aataatccgt
    14461 cactaacgct tgccagataa ccgccgcggc gcagacaccc acagcctgta caaaacgcaa
    14521 taccagcagc gtagcggcgt tttctaccca cagcatcccc agactaccta acgcaaaaat
    14581 tgtcaggccg attaataata ccggtttacg accataacgg tcggagagcg gcccccacag
    14641 aagctgggct gcggcaaaac cggcaaggaa cagactaagg ctggcactga cagcagacgc
    14701 aggcgtttgc aggtcggcct gtatggcggc gaaagcaggc agatacatat cggttgccag
    14761 aaaaccgagt acgctcaaac ccgccagcca gactaaaaat cttttcccag gttgcatgta
    14821 tattctctaa atctctcaat taaacaccgc cgcaaagtgt agggagtgaa attagtgttg
    14881 tgaaacggta atatttgatg actggtttca aaaattttgc aggcagacta tgtggtcaga
    14941 atattcactc gaagttgttg atgcggtagc gcgtaatggt agttttagcg ctgcggcaca
    15001 ggagttgcat cgcgttcctt ctgcggtcag ctataccgtg cgtcagctgg aagagtggct
    15061 ggcggtgccg ctgtttgaac gacgtcaccg tgatgtggaa ctgaccgctg ccggggcgtg
    15121 gtttctcaaa gaagggcgct ctgttgtcaa aaaaatgcag atcacccggc agcaatgtca
    15181 gcagatagcg aacggctggc gcggtcagtt agctatcgca gtggataata ttgtcaggcc
    15241 ggaacgtaca cggcagatga tcgttgattt ttatcgccat tttgatgatg tcgaactcct
    15301 tgtctttcag gaagtgttca acggtgtctg ggatgcgctt tccgacgggc gcgtggaact
    15361 ggcgattggc gcaacacgcg cgattccggt aggcggtcgt tatgccttcc gcgatatggg
    15421 gatgctaagc tggagttgcg ttgttgccag tcaccacccg ctggcgttga tggatggccc
    15481 gttcagcgat gatacgttgc gcaactggcc gtcgttggtg cgcgaagaca cctcgcgaac
    15541 gctacctaaa cgtattacct ggttgctgga taaccaaaaa agacttgtcg tgcctgactg
    15601 ggaatcatcg gcaacctgta tctctgcagg attatgcata gggatggtgc caacacattt
    15661 cgccaaaccg tggcttaatg aggggaagtg ggtggcacta gaactggaaa accccttccc
    15721 ggattcggca tgttgtctga catggcagca aaatgatatg tcgcctgcgt taacctggtt
    15781 gcttgagtat ctcggcgata gtgagacgct gaataaggag tggctgcggg agccggaaga
    15841 gactcccgca acgggtgatt aacgacgata gtcgcggaac gggccgtcag ccacggagcg
    15901 gcgttcaatc aaccgcggat gcacttcaat ggattgcggt tcttcgcgtt tattgacgat
    15961 acgatccaac agcatattga acgcagtttc gcccagcgaa tcttttggct gatggatcgt
    16021 ggtcagcgcc ggcgtaaaat agcgggcgtt gcgcacgtta tcatacccga tcagcgaaac
    16081 atcctgcggg acgcgcagac ccatttcatc agcagcacaa agtgcgccca ttgccatgat
    16141 atcgccacca cagaagacgg cagtagggcg atgcggctgc gacaggattt gctgcatggc
    16201 gcgataaccg gattcaggtt caaagtcacc ctgcacaatc cagctttccg gcactttgat
    16261 catcgcttct tccatcgcct tcataaaacc ggcaaggcgg cctgcgccgg tgttacgttc
    16321 cagtgggcca gggataacgc cgatttcgcg gtgaccgcgt tcaatcagat aacgcccggc
    16381 catgtagccg ccttcgaacg cgttatcaat gaccgcatcg gtgaagtcag cttttgcttc
    16441 accccagtcc atcaccacca tcgggatatg gcgatactct tccagcatcg ccagcaacgg
    16501 ctctgggtac tcagaacaca tcaccagcag accatcgacg cgtttttgcg ccatcatcga
    16561 cagataagcc cgctgtttct caagattgtt ccacgcattg cccagaatca gggtgtaacc
    16621 tttctggaag caattttttt caaccgcttc aatgatctcg gcaaaatagg ccgcttcgct
    16681 gctggtcgcc agcaaaccga tagacttggt gtggttaacc ttcagactac gcgccaccgc
    16741 gctaggggag tagtgtaatt ctttgatcgc tgcccacacg gcgttgcgcg tttcttcagc
    16801 gacgaaacgt gttttgttga tcacgtgtga cacagttgta gtggaaacgt ttgctcgttt
    16861 cgctacatct tttattgttg ccattccatt tcactccaga ccctaacttc agctcctgca
    16921 aaatcgcaag gtaaacgttt gcctttacac accttacgca caacaaaagt gttgcggtac
    16981 gccggaaaaa cgacacggag cgtcaggaag gggtcaatgg ccagtacgca aatagtgtaa
    17041 gcgtggaatt ttggccgatc ttgacgaaaa ggggaagtgg taaaaacact tatcattata
    17101 aatgacggga gattttttca tcacagtgtg taaaaatgcg taatatcact ttttgtgtgg
    17161 taataaaaaa ggagaaaact tgatgagcac cgaccttaaa ttttcactgg taacaacgat
    17221 tatcgtcctc ggtttgatcg tggccgtggg tttgactgcc gcgctgcact gatttcctct
    17281 cgagggagcg cgttctccct cgcatctcct tcctgtttgt tagcaaaatg gcaaaagtgt
    17341 tttctctttt tgttattttc atttttttgt gattgctgtc atgctttcgc cactttaatg
    17401 aatatcgcgt aatgacgcga agaggtggag ataaggcatg aaaattaact atccgttgct
    17461 ggcgctggcg attggcgcgt ttggtatcgg gacaacggag ttctcgccaa tgggcttgtt
    17521 gcccgtcatt gcgcgcggtg tggatatctc gattcccgct gccggaatgt taatcagtgc
    17581 ctatgcagtt ggcgtaatgg ttggcgcgcc gctgatgacg cttctacttt ctcatcgtgc
    17641 ccgccgcagt gcgttgattt tcctgatggc gattttcacg ctcggcaatg tactttccgc
    17701 catcgcgccg gattatatga ctctgatgct ttcacgcatt ttgaccagcc tgaatcacgg
    17761 agcatttttt ggtttgggtt cagttgtggc cgcaagcgtg gtgccaaaac ataaacaggc
    17821 cagcgcagtt gccactatgt ttatggggtt aaccctggca aatatcggtg gcgtgccggc
    17881 ggcgacctgg ttgggtgaaa ccatcggctg gcggatgtca tttctggcaa cggcggggct
    17941 gggagtgatt tcaatggtaa gtctgttctt ctcattacct aaaggtggcg caggggcgcg
    18001 acctgaagtg aaaaaagagc tggcggtatt aatgcgtccg caggtgttgt ctgcattgct
    18061 gacgacggta ctgggagctg gtgcaatgtt taccctctac acctatatct ctccggtact
    18121 gcaaagtatt acccacgcaa caccggtgtt cgttacggca atgctggtgc tgattggtgt
    18181 cggattctct atcggtaact atctcggcgg caaactggca gatcgttcag ttaacggcac
    18241 gttgaaaggc tttttgttgc tgctgatggt gattatgctg gcaatcccgt tcctggcccg
    18301 caatgagttc ggcgcagcta ttagcatggt ggtgtgggga gctgcaacct ttgcggtcgt
    18361 accgccgtta cagatgcgcg tgatgcgtgt cgccagtgaa gcgccaggtc tgtcttcatc
    18421 agtcaatatt ggtgccttta atcttggaaa cgcgctggga gcagctgctg gtggtgcggt
    18481 aatttccgct gggctgggat acagctttgt gccggtgatg ggggcgattg tcgcgggact
    18541 ggcattattg ctggtgttta tgtcagccag aaaacaacct gaggcagttt gcgttgccaa
    18601 cagctaatta aacaaaaacg cagaaggata accttctgcg ttacagttac tggcaacgtg
    18661 cgatttatct tgccggatgc ggtgttaacg cttcatccga gttggcatgg taagcgaagc
    18721 gcaccaggca atgttgcatt tgccatcagt tattatgcag cgagattttt tgctacgaat
    18781 tcccagttca ccagcgccca gaagtgctcc agatagccag gacgtgcatt gcgatagtcg
    18841 atgtaataag cgtgttccca gacatcaacg gtcagcagcg gagttgcatc ggtggtcagc
    18901 ggagtacccg cgttagaggt tgaaacgata gccagtttgc catcgctgtt tttcaccagc
    18961 caggtccagc cagaaccaaa gtttttgatt gctgcatcag taaactgtgc tttgaaatcg
    19021 gcaaagctgc caaaagatgc ggcgatagct tcagcgactt ttccagtcgg ttcgccaccc
    19081 gcgttcggtg ccaggcagtt ccagtagaaa gtatggttcc agacctgagc tgcgttgttg
    19141 aatacgccac cttcagagct gcgaataatc tcttccagtg atttaccttc aaacgcggta
    19201 cctttaatca ggttgttcag gttagtgaca taagtctgat gatgcttgcc gtagtgatac
    19261 tcgatggttt ccgcagaaat gtgcggtgcc agagcatctt tagcatatgg tagtgcaggt
    19321 aattcgaatg acattgctac tctcctttat tattaatttg catacgtaca atagccttat
    19381 tgtgcgtatg gatagggtag caaattaaaa ggtgagacaa aagatgaact taccctgttg
    19441 ccgtaacaac agggtaaagt tttagcgaag tgtttttggg gtcattaccc ggcgagcgcc
    19501 aacatagtgg cgctgccagt agtcttcact gagagaagtg atttgaattt cctgacccgt
    19561 acgcggtgac tgaataaatt tgccgttgcc gacatacacg ccgacatgat cggctgtacc
    19621 gcgtccctga gtacggaaaa agaccaggtc gccgtttttc agctcactac gttcgatagg
    19681 tgctgcatca cgcaggtgat acatttcatt cgccgtacgc ggaatacgaa ttttcaccaa
    19741 atctttataa gcgtaataaa ccaggccgct gcaatcaaaa ccggtacgcg gtgagctgcc
    19801 accccaacga tatggcttac caatttgctg catcagttta ttcattgcca ctttcgtggc
    19861 tttttgcacc ttcgctttgt gcgcgtcggc aagagttcct gacgcggcat ttttgacttt
    19921 cacacaatgc gatttatagc ccttacgttt ggtacacttt tcggtaaagc tggctgaggc
    19981 cgttttattg acggcgtgtt tgctgcgagt tttggcgttt ttagaagcgg taatggaaga
    20041 ttttttaacg gaagaagcgg ttttgctttt gatcgtactt ttactggcgg cttttttcgc
    20101 tgttttctgg ctggctttct gggtcttttt ggtggttgcc gtgcttttct ttttatctgc
    20161 ttttttggtg atatgagtag tagcagaact ctccctggct tgctttgaag catgggccat
    20221 aggcgttaaa ggcagggtgg taaaaagtaa agcacagagc gtgatcgaaa tacggtttat
    20281 ccgcgccact gagcgtccct ctgttaaggc aggtctatca tgcctgcgtc tgatttacaa
    20341 aacttgatga ttctaatgca tagaagaaga gaaagttaat agcttttatt gttaaaaaat
    20401 acgtttcgtc ggcgagcgca aaaaaatgca gcagtgaaag ctaaaaatgg tcataaatac
    20461 gccagtttgc cttgcgtcag ggaagaaacg aatatttgaa atgcaacaaa tagagcaaag
    20521 ggataaaata gcaaaagcgt gatgaaaatg ttattttccg atgccgcact gacccgctac
    20581 aatgtcagat tactgccgta aagaagtttg aggaagcaag acaatgagca ccactatcga
    20641 aaaaatccaa cgccagattg ctgaaaaccc gatcctgctg tacatgaaag gttcaccgaa
    20701 actgccgagc tgcggtttct ctgcccaggc agtccaggcg cttgccgcat gtggcgaacg
    20761 ttttgcctat gttgatatcc tgcagaatcc ggacattcgt gcggaactgc cgaaatatgc
    20821 taactggccg actttcccgc aactgtgggt tgacggcgag ctggtcggcg gttgtgatat
    20881 cgtgatcgaa atgtatcagc gtggcgaact gcagcagctg atcaaagaaa ctgccgctaa
    20941 atacaagtct gaagagccgg acgcggaata atccgtcctg caatactgac ggatgcgaac
    21001 gggcatccgt cagttcttta tctatcccca atccagccct tgtggcgaac ttgaaaatcc
    21061 cgcctcacgc agcagcgtaa acatcggcgt ttgccggacc ggtagatcat tcacttctgt
    21121 tagcgtaaag cgcagccgtg gttcgcgacg cagtgcggtg gtcagcgcct ggaaaatttc
    21181 tggggcgagt aattcatctt tttcctgcca gaccagcatt tttttgccgc cttgcgccag
    21241 atagagtaac aatttgccgc cagaaacgac caccagcgca cccgcccgac gcgttggaac
    21301 cagcgttgcc ggatgtgcag gccagggcag aagatttccc cacacattag ccggatcgtt
    21361 ggcggagagc gccactggtg tatagtggcg cgtttgcgtg gcttgtgtcg ccagatcgcg
    21421 caatcggtca atagtcagac gttcagcgaa ttgcgcactc cccaaacctt ctacaaaacg
    21481 acctcgcata attcgcccgg agtcttccat acttcgacaa agcgtttgca tcgatggaaa
    21541 cccgccaggg atattttcgg ctatcaccgc ctgacgactg atgatgccgt agcggtcgag
    21601 catattttcc gccagcgcca gcatcctttc ggtatcgttt agtggctcca cctgcaataa
    21661 cgaccagcgt ccggccagct ttggtgtgtt gtaagatacc cgcggcgaga cgggttgtgc
    21721 atagacagga cgcccacgac gagcccggtg actgcggcga gttgaggtgc gcgcgttgga
    21781 actgctgcgg gtgagggcgc gtaacggtgc ccaaatatcg ctggtgatga cgccttgcca
    21841 gaccagcgcc cataacgcct cttgcagtgc cgagggatca accgattcgc cgattttatc
    21901 gcgtatccgt tggctgattt gttgtgcaaa ccaggctcct ccgtcagcca gaaccgtgac
    21961 tatcgcttgc tgcagtgccg aacgattcgc ctgattcgct tcggcgggag tgaacgattc
    22021 tgcagcatat tcctgtagat gcagtgccac caggccgtca tcttcaccca gttttttttg
    22081 ccccgaccag ataaccgcac cggttgccag taattcatcg agcatttctg gcgaatagtc
    22141 gcgtacgcgg gcaggcagga tctggctttc ccagagtgag gcgggtaaac cgactccggc
    22201 aagctgttcg atcacccgca tgacgccatc cacgccctca taaacgcctg gcgatgttga
    22261 ggcaaagagt gccgggctac catcggtggc gggtaatacg ccctgacgtt ccagcagcaa
    22321 tcgcgcatag gtcgtggccg caacgggacg tgtcgcttct ctggcggctt gcagcgagcg
    22381 caaacgcaga cgacgaaata cttcaccgct cacccagatg tcttgttgca gattcatcac
    22441 cagcccctgt tcgcgcagtt gctgaagttg ctcttcgaca atggcaatac cgagactaaa
    22501 ctcatgagcc agttgttcag acgtgaccag agcatgagcc cggagatagc gcagaaagag
    22561 gtcgcgaagc gggtaactga ctcggtgcaa atcaatctct ggcagtgact ctggtagtcg
    22621 tacgccgaga gcatcgcgca gcctggcggc atcatccata caggcaagac gctcctgccc
    22681 gctaatcatc gtccggaaga ttcgttttac tgcaagaaga ttttccagat acgacgcaat
    22741 ctcttcactg ctgcctgtat gccgttgcac caggtcttca acggtcattg gccccagttc
    22801 gcgcagcagg tcgaacaatc cttcttcacc tttcgctctt ctgccaggag ccagtcgttg
    22861 caactcttct tccacctggc gaatgacctg cgggtcgagt aattcccccg gatcgacctg
    22921 tccgagtaga ttgcgcagta actcgctgtc cagcgacagt acggatgccc ggcgctctgc
    22981 cagcggggcg tcgctctggt acataaattc cgcgacatag ccgaacaata aactagcggc
    23041 gaaaggcgag ggcgtagtgg tcgtgacatc ggatatttga atttcgccac cgttcaggcg
    23101 acgcatcaaa cgttccagcg cgggaagatc ataaacatct tgcagacatt cgcgtagggt
    23161 ttcgagaatg accggaaaat ccggatatcc ctgagcgatt tccagcaact gactggcgcg
    23221 cagccgttgt tgccagagcg gggtgcgatg gcccggcgtg cggcccggca ttaataatgc
    23281 ccgcgcggcg cattcgcgaa aacgggcggc gaaaagtgcc gagctgccta ccgcctcgcg
    23341 gacaatttgc agcaactttt ctggttcaaa caaaaaaatc gcggcatcgg gcaatttacc
    23401 atcggtgtca ggaatgcgcg caacaatgcc gtcatcactg gcaaccacag acgcgtcagc
    23461 gccccatagt gcatgtattc gcccggcaat cgccagcgcc cagggttcat gcacccgtct
    23521 tccataggga gagtgcaaaa taatacgcca gtcaccaatt tcatcacggc accgttcgag
    23581 gaccagatga cgactccccg gaacgatgcc cgtcgcgttg cgctgctcgt caatcaaccc
    23641 ctgaatattg gcgatcgtat tttcttctgc caaccacggg ggaatagtcc cggaaaagaa
    23701 cgcgccatcc gccagtaaat ggagaaaatc gccgatcatc tcgcctaatt ctgccggacg
    23761 tccgttacct tcaccacgcc agaagggaag gcgggcagaa cgacccggag caggagtcac
    23821 aatcacctga tcgcgggtga tttgctggat ccgccatgag gtagcgccga gagtgataat
    23881 gtcgttcacc cgcgactcat ataccatctc ctcatccagt tcacccaccc gccgcgaacc
    23941 ggcctgttct tcaccttcgg gtaataacac gctatacatg ccacgatccg gaatggtgcc
    24001 accactggta accgccaaca gctgagcgcc aggtcgggcg gtcaatatcc cggtctccct
    24061 gttccagacc agttttggtc gaaaagcaga aaaatcgcca gagggatagc gcccggaaag
    24121 catatccagc gtggcgtcaa aaacacgtct tggcagatct ttccacggtg cggcacggcg
    24181 tacgcgggag taccattcgt ctgcctgtaa tgcatccatc gacgcggcgg caacggtttg
    24241 ctgcgcaagg acgtcgagtg gattatgcgg tggtgtcagg ttttccagcc tgcctgcgaa
    24301 catacactca acaatgactg cggaatcgac taagtcacgc ctggttcggg ggaaaaaaag
    24361 ccctttggag atacctccta cctggtgtcc ggcacgtcca atgcgttgta acccactggc
    24421 aacagaaagc ggcgttgcca cctgaatcac cagatccacc gcgcccatat caatccccag
    24481 ttcgagactg gaggttgcga ccacgcagcg taattcaccc gatttcagcg cctgttcggt
    24541 tattgctcgt tgttctttag agacggagcc gtggtgtgag cgggcaataa aaatgtcgct
    24601 actttgtaca cggttagagg ttgcgccgga ggtcgactcg aaatgggccg catcaacggc
    24661 gatagacggg gaacgctgta agcgtgcggc gtaaagctca ttcaatcgtg ccgtcagttt
    24721 ttccgccagc ccgcgtgaat tagtaaagac aatggtcgag cgatggcgca acacttcatc
    24781 aaggataccc gtttcaatat atggccagat ggagccttcc cggccggcat ggctgtcttc
    24841 gccggtgccg ctggcgaccg atgagacatc atccatattg gcgaccggta cgacaattcg
    24901 tatctgcgga tggcgcattg cgggcgggtt gactaccgta accgggcgat cgccgccaag
    24961 aaatgctgcc acatcgctgg ctgaacgcac agtggcagaa aggccaattc gctgtgctga
    25021 ggtgtggagc agcgcatcga gccgctccag acttaacgcc agatgcgcac cacgtttact
    25081 gcctgctacc gcgtggactt catcaataat taccgtttcg acgccgcgta gcgtttcgcg
    25141 cgcgcgggag gtcagcatca gatagagtga ttcgggtgtg gtgatcagaa tatccggcgg
    25201 attacgggtg agtttgctgc gttcctgtgc aggcgtatcg ccagtacgga tccccacgcg
    25261 aagattgact tccgtttcgc cgcgccgccg ccgttcatcg gcaataccct gtaacgggat
    25321 ctgcaagttg cgctgaacgt cggtgccaag ggcttttatc ggtgaaatat agaggatgcg
    25381 tgaggtttta cgtttatgcg cctcgcgggt atcttcgccg ccttcgcgga agagctgatc
    25441 gagggcgtag agaaatgctg ccagcgtttt cccggagcca gtcggtgcaa tcaccagcgc
    25501 atgttcgcct cgcgccgcta catgccaggt ttgcgactgg acagcggtcg gctgtttaaa
    25561 ggcgcgaaga aaccagtcgc gggttgccgg tgaaaacacg tccggcaggg gcgatgaaag
    25621 gtttggatta tctgccatta acgctattcc ccgtgaatat catgcatgat gcgtggaata
    25681 gcctcactat agatgcctga accattgcat gtcatcaggc atcgactcga ttacacctct
    25741 tcggcggcag gtagcggcca gcctcccaga cgtttccagc ggttgacgat ttcacaaaac
    25801 agcacagcag tgcgttcggt gtcgtacagc gcggagtgcg cctgggtgct gtcgaagtcc
    25861 atgccagcgg tctggcaagc ctttgacaat acggtttgtc cgagtgccag cccggcaagt
    25921 gcagcagtgt caaaagtggc gaaagggtgg aacgggttac gtttcagtga ggcgcgttct
    25981 gcggcggcca tcataaagct gtgatcgaaa ttggcgttgt gcgccaccat aatggcgcgg
    26041 ttacagccgc tcgctttaat gcctttacgt acaactttaa aaatttcgtg cagcgcctcg
    26101 tattcgctga ccgcgccgcg atcgggatcg ttcgggtcaa tgccgttgaa ggcgagggcc
    26161 tctggctgca aatttgcgcc gacaaatggt tcgacgtgga aatgtaatgt ggtgtccggc
    26221 atcagccagc cttgttcatc cattttcagg gtgatggcgg caatctcaag cagcgcatcg
    26281 gttttggcgt taaatccggc tgtttcaaca tcgatcacaa caggataaaa accacgaaaa
    26341 cggtcgcaca gaccggtaag ttgagcgtta tcggacatct gattctctta atacggataa
    26401 attgcagcgc gcattatgac aaatattact tgtcgatgca gtaaagatgc gggcgcgatg
    26461 agttcacgcc cggcaggaga ttagttgccc agaccgcgac cagcgtcttt ctcttcgatt
    26521 aattcaattt tgtaaccgtc cggatcttcc acaaacgcga taaccgtagt gccgcctttt
    26581 accggacccg cttcacgggt gacgttaccg ccgttctgac ggattttttc gcacgcttca
    26641 gcggcgttat caacgctaag cgcgatgtga ccataagcag tgccgagttc gtatttatcc
    26701 acgccccagt tgtaggtcag ttcaatcacc gcttcttcgg tttccgggcc gtagccaaca
    26761 aacgccagtg agtatttgta ttccgggttt tcgctggtac gcagcagttt catgcccagc
    26821 actttggtat aaaaatcgat ggagcgttgc agatcgccaa cgcgcagcat ggtatgaaga
    26881 agacgcatct ttttatcctc aaaatggcta acagattcaa aatgttgttt tagtatagcg
    26941 gcggctttgc gccgctcgca atgttggatt acagcgtcgg gtaatccgtg tagccttccg
    27001 caccgccgcc atagaaactt tcagcacgct gtggattaag ctcagctttg cgctgcaagc
    27061 gggcgaccag atccgggttc gcaatccagt cacgaccaaa tgccaccgca tcaattaacc
    27121 ctttgccaat cagcgtttcg gctttttcta ccgtgtatgc acctgcgccg ataatcggac
    27181 cgtggaaacg ggcgcgtact ttttcgcgga acgcatcagt atacggttca cctcccgccc
    27241 aatctggttc tgacatatgc agataagcaa tgccgcgttt acccagttgt tcaatcagat
    27301 acagtgcatc ggcttcttca ttcgggccgt tatccgtgtt ctggaaagta ccgattggcg
    27361 aaacgcgaat gccaatgcga tcggcccccc attcttcaat ccctgcatcg accacttcca
    27421 gcaccagacg tgcgcgattt tccacgctgc cgccgtactg atcggtacga tggtttgaag
    27481 aaggagaaag gaactgatgc agcaaataac catgagcaga gtggagctct accagatcaa
    27541 aaccggcttc acgcgcgtta gcaatggcct gacggaaatc attgacgata cccggaatct
    27601 cttccagttc aagcgcacgc ggcatggatg tttcaacacg gatcgcctga ccattttcat
    27661 cgcgcagaga agtacgtgtt cccgcgctaa gtgcggaagg cgctaccggt gcctgaccgc
    27721 caggttgcag gctggcgtga gaaatgcgtc cggtgtgcca cagctgtacg gccatatgac
    27781 cattttcagc atgaacgcca gcggtaattt ttttccatgc ggcaatttgt tccggactat
    27841 ggatgccagg tgcaccagca tacccttttg cctgggcaga aatttgcgtg gcttcgctaa
    27901 taatcaaacc ggcactggca cgttggcgat agtattccgc catcaacggg gtagggatgt
    27961 cacccggttc aatactgcgc agacgcgtca gcggtgccat aaaaatacgg tttgccgccg
    28021 tgatcgcgcc cactttcagt ggggaataca gtttttcaga tgacataaag gctcccggtc
    28081 gtctggtgat aaaaaatgcc tgctaaacgg caggcgtcgc aataatggtt tttacatgtg
    28141 ccagcgcgtt ttccagtggc tcggaattgc gcgaaatttt ggcctgcaga ttcgcaccca
    28201 gccatagtgc gtaaagcact tgtgcctgtt gcagcggttc gccacaaaag gttaaacaat
    28261 ggctatcacg gccattttcc agcgcctgcg agagcagaac gatcacgccg cgagcgcctt
    28321 tatccatcgc gctacgcata tcttctgaca gatcgcacac ttcggcagag agttttactg
    28381 tcaggcaacc actgatggtt ccatgttggc aaaactggtt cagtgtttgc tggtaataag
    28441 ccagtatgcg gtcgcggtag ttaccttcgc cggattgcag caactcagtc agtcgctggt
    28501 gatatgcggc gtaatggcgc tcaagcatgg caacgccaaa cgcttcttta gagcgaaagt
    28561 agtgatagaa ggaccctttc ggcacttcag cagtttttag taattcgctt agccccatcc
    28621 cggtgaatcc acgttgcagg caaagttgct cgcccgtcgc caggagatgt tcgcgagtat
    28681 catgttcggt gtgtttgttc atgcgttgga gtgtagtaga ccagtcggtc tagtgcaagc
    28741 aaagttgcta ctttgggcat taacagcttc aataatggta ataatcttat ttgaggaggg
    28801 cgaagtggcg gagcaattag agttttttcc cgtccagagc ccgtgccggg gaatttgcca
    28861 gtctgacgaa cgcgggtttt gtcgcggctg ttttcgtagt cgggatgagc gttttaactg
    28921 gaataaaatg agcgatggcg aaaaacaaga ggtgctgcgc ctttgtcggc agcgattaat
    28981 gcgcaaatta cgcgcaaata agccagcgtc atcagacgaa ccggagcaac catcactctt
    29041 ttgagcctaa tcttgcgtat actcctctca tatctgataa gaggaagcga ttatggttca
    29101 gcgtattact attgcgccgc aaggcccgga gttttcccgt tttgtgatgg gctactggcg
    29161 attgatggac tggaatatgt ccgcccgcca gctggtcagt tttattgaag agcatctgga
    29221 tctcggcgtg accaccgtgg accatgctga tatttatggt ggctatcagt gcgaagcggc
    29281 gtttggcgag gcactgaaac tggcacctca cctgcgtgaa cggatggaaa tcgtcagtaa
    29341 atgcggtatc gcgacgaccg cgcgtgaaga aaacgtcatt ggtcattaca tcactgaccg
    29401 cgatcacatc attaagagcg ccgaacagtc gctaattaat ctcgcgaccg atcatctgga
    29461 tctgctgtta atccaccgac cagacccgtt aatggatgcc gatgaagtgg cggacgcgtt
    29521 taaacatctg catcagagcg gcaaagtgcg tcattttggc gtatcgaact ttacgcctgc
    29581 gcaatttgcg cttttgcaat cacgcctgcc gtttaccctt gccactaatc aggtggaaat
    29641 atccccggta catcagccgt tactgttgga tggcacgctc gaccaactgc aacaactgcg
    29701 tgttcgtccg atggcgtggt cctgccttgg tggtggtcgt ctgtttaatg atgattattt
    29761 ccagccgctg cgtgatgaac tggctgtggt ggcggaggag ttaaacgcgg gctcgattga
    29821 acaggtggtt tacgcctggg tattacgttt accgtcgcag ccgctgccaa ttatcggctc
    29881 aggtaaaatt gagcgcgtac gggcagctgt cgaagctgaa acactgaaaa tgacccgtca
    29941 acaatggttc cgtatccgta aagcggcatt gggttacgac gtaccgtaag cacttttagg
    30001 aatagccgcc gttcaaaaat gtgtcactgg tttacactta ttcaggaatg cacaatgaac
    30061 ggaggtccta tgaaacgttt tagtctggcc attctggcgc tggttgttgc gaccggcgca
    30121 caagctgcca gtgaaaaagt cgagatgaac ctcgtcacgt cgcaaggggt tgggcagtca
    30181 attggtagcg tcaccattac tgaaaccgat aaaggtctgg agttttcgcc cgatctgaaa
    30241 gcattacccc ccggtgaaca tggcttccat attcatgcca aaggaagctg ccagccagcc
    30301 accaaagatg gcaaagccag cgccgcggaa tccgcaggcg ggcatcttga tccacaaaat
    30361 accggtaaac atgaagggcc agaaggtgcc gggcatttag gcgatctgcc tgcactggtc
    30421 gtcaataatg acggcaaagc taccgatgcc gtcatcgcac ctcgtctgaa atcactggat
    30481 gaagtcaaag acaaagcact gatggtccat gttggcggcg ataatatgtc cgatcaacct
    30541 aaaccgctgg gcggtggcgg tgaacgctat gcctgtggtg taattaagta attaagaggc
    30601 cagcgtacct tgcggtggtg cttgctcaag ttgcgaaagc gagcagtaca gccgccagac
    30661 aattgccgcc agctcacggg cggcaggttg atgatggcgg gcaaggctgt cgcaaatccg
    30721 ctgtaattct tcaagtgtgg ccgccagtga tttttgctga acgccacgct cactcatcac
    30781 tccgcgcaac agtgaaatac agttatcccg tactcgtgat aacggatcgg aacgcgattc
    30841 ccagtcgcgt aattgccaga caacatgaga acagttcagc agcactacac cccagcgtaa
    30901 taaccaacgg cgagccagcg catcctggct gttactcaac tgactgacgt gatgataagt
    30961 gagcgattca aattcgcttt cactcagtgt tggatggcgg cttagctgat cgacaaaatc
    31021 ccggcgcagc gcgcgaatat gacgacggct tttacgagca cccgaccctg gacgcaaaat
    31081 ggcgaacgct aaccacgcca gcgcaacgcc aacgattttt gccagattat cgttaagaaa
    31141 atcagcaaaa tcatacaccg ggggattagt gacagcgata aacgaaccca taaaaacaat
    31201 cagttgcccc cacagtgcgg caaatttggg catctgcaat ttaagaagct gcattgtcgc
    31261 cagcagtgga aagagaaaca gtaaaaattg ccacagatcg ctaatctgga ccatcaggcc
    31321 aaatttgacc acaaagctga atagcgaaag taacaccaga gtgcgcatca gtagtgacaa
    31381 cgacttaaac ggtgctgcga cggcggagta gagtacgcag ctaattgctg ccagcgttaa
    31441 tgcattggca ccggcatccc attgcgaagc aatactccat gcgccaatca tcatcaacgt
    31501 acaaaatgta cgcagtccgc tccacatagc ttcggcgtta tcggtatgac gagccaggcc
    31561 ggatgtccgt gggagtctgg tatgatcatc cacaccgctt tgcagacgat gtaattcctg
    31621 actactttgc agataaaggc ggcaaaaata acgtagtcgc tgccagaagg cgacgtgccg
    31681 gtagtcggcg acgttggtcg ggcgtagcgg ggcgataata cgtgcgacgg tgtaaacatc
    31741 tgtttgcgaa ctggcgagcg ccgtcagcag ctgttcgaga atttctcgtg tggcacatgg
    31801 cggtgagggc cagttgagca acatacgtcg caggctggag atgacactgg tcatacgtaa
    31861 ttgctggtgg agcagcgcat taaggcgtgc gttttgctgg cgaaaacgat agtggctcca
    31921 gaaagcctgg atacggagca aattcatggt cagtatctgt ccaatcaccc cctcatgtgc
    31981 tgcacgaatg gcatcggttg tttcaggctg ccagagtaaa ctggcatgtt ccagtaatcg
    32041 ggcgtgcatg tttttcaatg cggttaaaag ggcagtagca tcggaactgc tcggcaggat
    32101 catcatcatc atgccgccgc acaaaatacc gacaatcacc tcgcaaacgc gcgcctgagc
    32161 gatatcccac agctggctgg cctcagtaat attaaccatc ggaaaggcga tgatggcagc
    32221 cgtgtagccc gccagttgaa atgcatacgc gacattattc gtgaagtgcg cacaggccca
    32281 ggtacaaaag ccaagccacg ccgccatgct caaaagaaaa aaccacggct cattgagcgt
    32341 atgtccggca agaagcagtg ccgcaatggc tccgagcaaa ctgccagcga tgcgtccgag
    32401 gcttttgctg atgacaccgc caacggtggg aaagctaacc acggcagccg aggtcatcgc
    32461 ccaatagggt tcatccagat ttaaataata ggcaaccgtc agcgccagac acatggcaat
    32521 ggtattgcgt aacgcataac gccattgcgc cagcgtggcc ctgaaccagg gcaaattgcg
    32581 caaggaccat gacgatgcgt tcattattga cctacggcaa tgctacaggt ggtgcccgac
    32641 accagcgtga catcgcctgg gactttatcc agcgcaaagc ggacgggaac gcgttgggca
    32701 agtcgaaccc aggggacgtt aggtttcaca tccggaataa ggcttgagtc actttcaacg
    32761 ctctgatcat aaatcgcccg accgatactg gaaacgtgac cctgtaacgt tttgttatca
    32821 ctatagaggg taatttgtgc tggtgcacct tcgcggatat ggcgcaactt ggtttcttca
    32881 aaataaccaa tgacgtaaaa cgagtggctg tcgacaaggg caaacagcgg tttcccggtg
    32941 tcagcgtaat cgccgatgcg ggtagtgagg ttagtcaccc atccactcac cggggcgcga
    33001 atttccgttt gcgccagttg ccattgtgcc tgtttcagcg tcgcctgtgc ggcatcaaca
    33061 ctggcctgca tcgctttaac attgaggtta gcggtatcga gttcttcggc agaaataaaa
    33121 ttttgtgaga gatgacgacg acgattcgct tcgttattgg cttttgcgag gtcagattgc
    33181 gctttcgcca gctgagcctg tgcgttcagc tcggcgatct gaaagggcgt tttatcgatc
    33241 gtgagcagaa gatcccccgc gttaaccagt tggttgtctt ttatattcag ctcaacaata
    33301 cgtcccgaca cctgaggggt gatagaaacc tgttcagcgc gtattttccc gtcgcgtgtc
    33361 cacggagatt gcatgtaata attccacagc caccatccgg caagaaccgc aactaccgct
    33421 acaatgattg ttgagaaata cttaattgtt ttaattgaca tagttaccac gcaatcagta
    33481 ttgccagggc aaggcaaacg caaatcgcaa acagcgataa atccattaac aagggatgcc
    33541 agatgtcacc ggcgtagatc cagccacgaa gcaggcggtg tacgacaagc cagatgacga
    33601 atccaaacgc gaaggctttg aaaaacggag gaaagtagac ggacgcaccg aacaccagat
    33661 cttgtaaggg caatcctgtt gcattgagca taaacttcac aaagtagatt cctttacgac
    33721 cgaatatatt gcgttagatt aataaatatt ctttaagtgc gaaaaattta cgcgcaattt
    33781 ctgaaaaata tgtctgacgg taaccaaatg cagcaataca tttgttttag caatacaatt
    33841 gctgcacact attctaaaag ccgcataata tcttagcaag ctaattataa ggagatgaaa
    33901 ttggaatcgc cactaggttc tgatctggca cggttggtgc gcatatggcg tgctctgata
    33961 gaccatcgcc tgaaaccgct ggagttaaca caaacccatt gggttacgtt acacaatatc
    34021 catcagttac ctccagacca gtcgcaaatt caactggcaa aagcgattgg catcgagcag
    34081 ccatcactgg tccgtactct ggaccaactg gaagaaaaag ggttaatttc gcgtcaaact
    34141 tgtgccagcg atcgtcgggc taaacgtatt aaactgacgg aaaaggcaga gccgctgatc
    34201 agcgaaatgg aagctgttat taacaaaacc cgcgcggaaa ttttacatgg catctccgca
    34261 gaggaactgg aacaactgat taagctcatc gcaaaacttg agcataatat cattgagtta
    34321 caggccaaag ggtgaaatga agggggcgtg tggtcagtaa cctgaccaca cgcaaactta
    34381 tttagcgcgg ggaaacggtc acctgactgc cattgctggc cagtacgaca cgttggcccg
    34441 gagagaaacg agtgttgcct tgtttctgta ccaccatgat ggtattacca tcgtctttac
    34501 gaatttccag ctcgacaccc tgcgttttgt tcattgcact ctgtacgccc tgaccggcta
    34561 cgccacctgc aacagcacct gctgcagtag ccagagaacg cccggttccg ccaccaacag
    34621 tattccccag gaaaccacca agaacagcac cgccaattgc gccgataacg ttggaatcat
    34681 caccgccctg aatctgtacc ggacgtacgt taacgatggt gccatagctg acattctgta
    34741 cttgtttcgc ttcagaagcg gtataaacat cccctgacag ggtgtcgtta ttaacacaac
    34801 cgacaagaga cagacctacc attgaaacaa ccaatacgcg tttaatcatt gaaaaatctc
    34861 ctgttcacca tgaaacgcca cgcaagcatc cctcatggtc aaagtatatg gcatatgtga
    34921 atccatggtt acatgttcta ccaaatatga gcaaatcata atgaaagtag gatcaaccaa
    34981 ttataaacaa aatattcatg atgattattg atttgaagat aatgttttac taccaacggt
    35041 aaaaatcggt cattccgcat agtattcacg aaaagtttat gtttgagtgg tgcgcaaacg
    35101 tcagattgaa aaaggatgaa ctatgaaatc gggccgcttt attggcgtta tgtcaggcac
    35161 cagccttgat ggtgttgatg ttgtgttggc gacaattgat gaacaccggg tcgcacagct
    35221 ggcaagtttg agctggccga tcccggtgtc tctgaaacag gctgtactgg atatttgcca
    35281 gggccagcag cttacacttt cgcagtttgg acagcttgat actcaactcg ggcgactttt
    35341 tgctgatgcg gtcaatgcct tgcttaagga acaaaacctg caggcgagag atatcgttgc
    35401 gatcggctgt cacggtcaaa ccgtctggca tgaaccgacg ggcgtggcac cacacacttt
    35461 acagattggc gataacaatc aaattgtggc acgcaccgga attacggttg tcggtgattt
    35521 tcgccgtcgc gatattgcct tgggaggaca aggcgcaccg ctggtacctg cgttccatca
    35581 tgcgctgctg gctcacccaa ccgagcgacg aatggtgctc aatattggcg gcatcgcaaa
    35641 tctgtcactg ctcattcctg ggcagccggt tgggggctac gataccggtc ctggtaacat
    35701 gctgatggat gcctggatct ggcgtcaggc aggtaaacct tacgataaag atgccgagtg
    35761 ggcacgggcg ggtaaagtca ttctcccact gctgcaaaat atgcttagcg acccgtattt
    35821 ctcgcaacct gcaccgaaaa gtaccggacg cgaatacttt aactacggct ggctggagcg
    35881 ccatttgcgc cattttccgg gtgttgatcc ccgagatgta caggcgacac tggcagaact
    35941 caccgccgtg accatttctg aacaagtttt gttgagcggt ggctgcgaac gattgatggt
    36001 atgtggtgga ggaagtcgta atccgctact catggcgcgt ctggcggcat tactgccagg
    36061 cacagaagtc accaccaccg atgccgttgg cattagtggc gatgacatgg aagcattggc
    36121 tttcgcctgg cttgcctggc ggacgctggc gggattacca ggaaatctgc cttccgtcac
    36181 tggcgcaagc caggagacgg tactcggggg tattttcccc gctaacccgt gatatcagag
    36241 ttaactgaat ttttcttacc gcgtctgccg ttacacttgg aacgttaggg agggcgtatt
    36301 gccctccaga ccaggaaagt cttcgggata tgtctatgaa aaaactgtta ctgatctgtt
    36361 tgccggtcct tctcaccggc tgtagcgcgt tcaatcaact ggttgagcgc atgcaaaccg
    36421 atacgctgga ataccagtgt gatgaaaaac cgttgacggt caaactgaat aatccgcgcc
    36481 aggaagtcag ttttgtttac gataatcaac tactgcatct caaacagggc atttctgcgt
    36541 ctggtgcacg ttacaccgac gggatctatg ttttctggtc gaaaggcgag gaagcgactg
    36601 tctataaacg cgaccgcatc gtcttgaata actgtcagtt acaaaatcca cagcgttgag
    36661 atttttccag gggcggcgca caatagcgtc acccactgac aatccgtaaa gaaaaccatg
    36721 tctgataacg acgaattgca gcaaatcgcg catctgcgcc gtgaatacac caaaggcgga
    36781 ttacgccgcc gcgatcttcc cgccgatcca ttaacccttt ttgaacgctg gctctctcag
    36841 gcgtgtgaag ccaaactggc ggaccctacc gcgatggtgg tcgctaccgt ggatgaacat
    36901 ggtcagcctt atcagcgcat cgttttactc aaacattacg acgaaaaagg catggtgttt
    36961 tacaccaacc tcggcagccg taaagcacat caaatcgaaa ataatccgcg cgttagcctg
    37021 ctgttcccgt ggcataccct tgagcgccag gtgatggtga tcggtaaagc ggaacgactt
    37081 tcgactctcg aagtgatgaa atattttcat agtcgtcccc gtgatagcca gattggtgca
    37141 tgggtttcga agcagtccag tcgcatttct gcccgcggta tccttgaaag taaattcctg
    37201 gagctgaagc agaagtttca acagggcgaa gtgccattgc cgagtttttg gggcggtttt
    37261 cgcgtcagcc ttgaacagat tgagttctgg cagggcggtg agcatcgcct gcatgaccgc
    37321 tttttgtacc agcgtgaaaa tgatgcgtgg aagattgatc gtcttgcccc ctgaaaagat
    37381 gcaaaaatct tgctttaatc gctggtactc ctgattctgg cactttattc tatgtctctt
    37441 tcgcatctgg cgaaaagtcg tgtaccggca aaggtgcagt cgttatatac atggagattt
    37501 tgatggcaag cagtaacttg attaaacaat tgcaagagcg ggggctggta gcccaggtga
    37561 cggacgagga agcgttagca gagcgactgg cgcaaggccc gatcgcgctc tattgcggct
    37621 tcgatcctac cgctgacagc ttgcatttgg ggcatcttgt tccattgtta tgcctgaaac
    37681 gcttccagca ggcgggccac aagccggttg cgctggtagg cggcgcgacg ggtctgattg
    37741 gcgacccgag cttcaaagct gctgagcgta agctgaacac cgaagaaact gttcaggaat
    37801 gggtggacaa aatccgcaag caggttgccc cgttcctcga tttcgactgt ggagaaaact
    37861 ctgctatcgc ggcgaacaac tatgactggt tcggcaatat gaatgtgctg accttcctgc
    37921 gcgatattgg caaacacttc tccgttaacc agatgatcaa caaagaagcg gttaagcagc
    37981 gtctcaaccg tgaagatcag gggatttcgt tcactgagtt ttcctacaac ctgttgcagg
    38041 gttatgactt cgcctgtctg aacaaacagt acggtgtggt gctgcaaatt ggtggttctg
    38101 accagtgggg taacatcact tctggtatcg acctgacccg tcgtctgcat cagaatcagg
    38161 tgtttggcct gaccgttccg ctgatcacca aagcagacgg caccaaattt ggtaaaactg
    38221 aaggcggcgc agtctggttg gatccgaaga aaaccagccc gtacaaattc taccagttct
    38281 ggatcaacac tgcggatgcc gacgtttacc gcttcctgaa gttcttcacc tttatgagca
    38341 ttgaagagat caacgccctg gaagaagaag ataaaaacag cggtaaagca ccgcgcgccc
    38401 agtatgtact ggcggagcag gtgactcgtc tggttcacgg tgaagaaggt ttacaggcgg
    38461 caaaacgtat taccgaatgc ctgttcagcg gttccttgag tgcgctgagt gaagcggact
    38521 tcgaacagct ggcgcaggac ggcgtaccga tggttgagat ggaaaaaggc gcagacctga
    38581 tgcaggcgct ggtcgattct gaactgcaac cttcccgtgg tcaggcgcgt aaaactatcg
    38641 cctccaatgc catcaccatt aacggtgaaa aacaatccga tcctgaatac ttctttaaag
    38701 aagaagatcg tctgtttggt cgttttacct tactgcgtcg cggcaaaaag aattactgtc
    38761 tgatttgctg gaaataatgc attaagtgga aagggggagt gagaaatcac tccccctggt
    38821 ttttatacag ggaacatgat gaaaaatatt ctcgctatcc agtctcacgt tgtttatggt
    38881 catgcgggta acagtgcggc agagtttccg atgcgccgcc tgggcgcgaa cgtctggccg
    38941 ctgaacaccg ttcaattttc taatcacacc caatacggca aatggactgg ctgcgtgatg
    39001 ccgcccagcc atttaaccga aattgtgcaa ggcattgccg ccattgataa attacacacc
    39061 tgtgatgccg tattaagtgg ctatctgggt tcggcggagc agggtgaaca tattctcggt
    39121 atcgttcgtc aggtgaaagc agcaaatccg caggcgaaat atttttgcga tccggtaatg
    39181 ggtcatccgg aaaaaggctg tatcgttgca ccgggtgtcg cagagtttca tgtgcggcac
    39241 ggtttgcctg ccagcgatat cattgcgccg aatctggttg agctggagat actctgtgag
    39301 catccggtaa ataacgtcga agaagcggtt ctggcagcgc gtaaactcat tgcgcaaggg
    39361 ccacaaattg tgttggttaa acacctggcg cgggctggct acagccgtga ccgttttgaa
    39421 atgctgctgg ttaccgccga tgaagcctgg catatcagcc gtccgctggt ggattttggt
    39481 atgcgccagc cggtaggtgt tggtgatgtg acgagcggtt tactgctggt gaaactgctt
    39541 cagggggcaa cgctgcagga ggccctggaa catgtgaccg ctgcagtata cgaaatcatg
    39601 gtgaccacca aagcaatgca ggaatatgag ctgcaagtgg ttgctgctca ggatcgtatt
    39661 gccaaaccag aacattattt cagcgcaaca aagctctgaa atatctcatt tcggcccaca
    39721 acgacctgtg ggccgatggc tattacttta agccttccgc tgacagcgcg tcttgtactt
    39781 ccggacgttc agccatacgt tgcataaatg ccgcaatgtg ctctaaccct tccagattca
    39841 gtttcaccgc gtatgcccag cgcagaaccg taaacagata ggcatcagca attgtaaatc
    39901 gttgcccgca gatccagtgc tcatccttca gagcatcgtt cacatattgc agcttcttct
    39961 ccagctgcgc gcgaactgtt ggtttgtact cttccggtgt atcagggcga aacagaggtg
    40021 tgaagccttt atgcagctcg gtggcgatgt aattcagcca ttcgatggtt ttatagcggg
    40081 aaatactgtt taccggtgcc agcaactggc ggtcggggac gctgtcggca agatactgca
    40141 taatcgctac gccttccgtc agcaaagtac catcgtccag cagcaatgca ggtacctgtc
    40201 ccttagggtt aacggcaaag taatcgtcac cgttttcgag acgttttttc attaaatcca
    40261 cactgacgag cgtaaaatcc tttccgctct cacgcagggt gatatgggaa gcgagagagc
    40321 aggcgcccgg tttgtagaac aatttcatcg gtaactcctt ttgtctgtgg tttaaagtat
    40381 ggtagtgcgt tgctgaacaa aaaaaaagcc gctaacgaaa agttagcggc ttgtaaaaga
    40441 gtttccctga attttacgct acagcagctt tcgccgcttt gtctgcagcg tcatcctgcg
    40501 tcatgcggtg cagtttcggc gcggtcagca gcatcagtac agcaataacg gcagtagcga
    40561 caccaatctg caagaatacg cgaccataga cttccagtga catcagcgga tcggtaacgt
    40621 tatccggcac agccatcata cccgcaacat aaccaccaat caggtttgca ccggcagtgg
    40681 tcaggaacca gctacccata atgaagccca tcagacgctg cggaacgagt tgtgcaacca
    40741 ttgccagacc cagaccagag atcataagtt ccccgatact ctgcaggcca tagcttgcga
    40801 ccagccagct tacagacacg atacctgcgt cagacgcgaa ttttgcaccc agcggcagaa
    40861 tcaggaacgc accagaacac atcaccatac cgattgcaaa cttagttggc atcggcaggg
    40921 tatcgcccat cttgttatag atagcggcca gaatcgggct accaatgatg atccagaacg
    40981 ggttcagtgc ctgatactgc tcaggttcta cggccaggcc cagaatggtg tgttcaacgt
    41041 tacgaatcgc aaagaagttc agtgacgttg gcatctggct gtacagcacg aagaagataa
    41101 tggcttcgag catcaggatg aaggcaacga tcattttacg acgcgcagca cctttcatcg
    41161 cgaaggcttc tttaccgaag ataaccacaa taccgaaggc aacaacgccc agcgccatac
    41221 gcgcaacttc ctgattgtgc agcagccagg tggcgatagc gatcagtgcc acaacaccaa
    41281 taatggtcag cagcaggtta cggtagttga taggctcgaa gtctggtttt gaaccgtact
    41341 gtttaaccca gcgttggcag aaggcgaagt taacgatagt gatcagcagg cctacaacgc
    41401 tcaacgcaaa cgcaacactc cagccgtatt tcgcggccag ccacggggtg gcgatcatag
    41461 agaagaaaga gccgatgttg acggacatgt agtacatggt gaatgcaccg tccagacgcg
    41521 ggtcgttttt ctcatagcat gtagaaagca gagaagacgg gttagcttta aacaggccgt
    41581 taccgaccgc aatagccgcc atacccatat aaacgatacc ggcgtcgtga ccagaccagg
    41641 caaccagagc ataaccaatc gccagcacaa tagcgccaag cataattacg cgtttagtac
    41701 ccagtacctt gtcacctaac cagccgccga tagcgaccag accataaacc agggcactaa
    41761 aggaagagaa aagggtgatt gaatccgctt cagacatacc cagttgttta accaggtaaa
    41821 cagccataat tccttgtagg ccgtaataac caaaacgttc ccataactca atcgagaaga
    41881 tgagatagaa cgccttcggt tgtttgaaag cgttcaaact gacgctttca gttggttttt
    41941 ggtttgcagt ggacactttt acctcttttt ttacatccca tattaacggg ggtgctttct
    42001 cgcgttatgc ctaaaaaaca tacgcgcaat tgttatagtg ggaggggaaa cggcaggtaa
    42061 tgttcactat cttgcacttt cagacaagtc ctttgtaata atctgttaca tataactgag
    42121 gcggggaatt caaccattcc gggaaatgtt atccagcgtt aaatttttca aaaaacgtaa
    42181 cggcagattt tttcactacc ctgagagggg gatgatggct taacggcgat atgttctgct
    42241 atttagcgtt tttcctagtg agatagtcca gtttctgaaa aatagccagt gtaatgtttt
    42301 gtaggtcaat atttgttgcg tgtgtaaatt taaatgattt taacctagtt cttgcaatgt
    42361 ttttacattg ccacagcatc gtgatcttga tcacgaatga atagaaaatt tcgctattaa
    42421 aaaaacaatt aacctgcatt tttggttatc cacaaacgga ttacctgatg ataacgctaa
    42481 gcaatggcat cattgacgtt aaaaagatgt tttaaaagag gataaagaaa ggttatcaat
    42541 ggggtaatcg gtgttacccc ttttcttcag atgtcaactt tctctttgta ttcacaaaga
    42601 tcttcaataa tacaagagcc acagcgaggc ttgcgggcaa tacaggtata acgcccgtgc
    42661 aggatcaacc aatggtggca gtcgacttta aactctgccg gaaccacttt cagtagcttt
    42721 tcttctacct gttcgacgtt tttccccagc gcaaattgag tacgattaca aacgcggaaa
    42781 atgtgcgtgt cgacagcaat agtcggccag ccgaatgcag tgtttaatac gacgttggct
    42841 gttttacgac ctacaccagg cagggcttca agcgcagcac gatcttccgg aacctcgcca
    42901 ttatgctgtt ccagtaagat acggcaggtt ctgatgatat tttctgcttt gctgttataa
    42961 agcccaatgg ttttgatata ggttttcacc ccttcgacgc ccagttcaag catcgctgca
    43021 ggtgtattag ccaccgggta gagtttcgcc gtcgccttat taacactgac atcggtcgcc
    43081 tgagcggaaa gcagtacggc aatcagcaat tcaaaaggcg aactgaaatt aagctcggtg
    43141 gtgggatgag gattgttctc acgcaggcga gtgaggatct ccaggcgttt tgctttgttc
    43201 atcagacatt ccctgtttca ccgtttggca atgcacgttc ggcagctgct tcagtacggc
    43261 gctttttcat tctttcatca atcaggtatt tccctgccag catcagtccc aggccaataa
    43321 atgcacctgg tggcagcatc gccagcagga aaggggagtc ggtgtggaaa atttccacgc
    43381 gtaatacttt tgcccagcta cctaacagcg catctgcacc gtcaaacaac gtgccattgc
    43441 cgataatttc gcgtagtgaa cccagcacga acatggcgca ggttgcgccc ataccaattg
    43501 aaaagccgtc cagtgccgaa agcgccggac cttttttggc ggcgaaggct tcagcgcggc
    43561 ccacaacgat acagttagtg acaatcagcg gaataaaaat ccctaatgat tgatacaggc
    43621 caaaggcgta ggcgttgatc agcatctgta cagcgctgac caccgaggcg atgatcatca
    43681 cgtaaatggg aatgcggatc tcggctggcg tccagtgacg cagcgtcgaa atggtcaagt
    43741 tggtcagcgt cagtaccagc gtagtcgcaa gtcctaaacc cagagcgtta gtggcagtgg
    43801 acgtgaccgc caatagcgga cagaggccga gcagctggac cagcgcagag ttgtttttcc
    43861 acaacccctg aacaataacg tctttaattt cgctcacgct ttattctcca caggcaggaa
    43921 gttgagaaag ttgtgacggt aacgtctgag cgtacaatcc ggcgcgtttt accgcattaa
    43981 ccaccgcgcg cggagtaatc gtcgcgccgg tgaactggtc gaaatcacca ccatctttct
    44041 tcaccgccca gtgcgcatca tccgcaccac tgattttttt acccgcaaaa tgggtgatcc
    44101 agtcagaaag gcgcagttcg attttatcgc caagccctgg cgtttcgtgg tgctctgtca
    44161 cgcgcgtgcc aagtaccgtg ccgttaaaat cggctcccac cagcagctga atcgcaccgg
    44221 aatagccatc tggcgcggtt gtttccagaa cggcggctac cggtttgtca tcctgtttgg
    44281 cgatgtaaac ccgatgctca cctttaccta actctggcgc agttaccaga tagcaactct
    44341 gtgccagcgc attgttatag cgttcggctg gcagcacctg atcaaataac gctttttgtt
    44401 gcagactggc ctgttcagca atcgtcgttt tggtcatctg gttgatggcc gcagttaacc
    44461 ctgtagaacc cgctgcaaat agtgccaacg taatgccgtg ttttcggata gttttcagca
    44521 tggtttaccc tttgcgatgg ccgtagacgc gcggacgcgt gtagtaatcg atcagaggaa
    44581 ccgtgatgtt cgccagcagg acggcaaaag ccacgccgtc aggatagccg ccgaaactgc
    44641 ggatcaacca gactaataag cccgccagcg cgccgaaaat aagacgacca cgattggtcg
    44701 tagaagcggt aaccgggtca gtcaaaataa agaatgcgcc gagcatagtc gcaccagaca
    44761 gcagatgaat ttggggtgat gccagtgttt ctggtgagaa caaccagccc aacgttgcgc
    44821 ataacgccag cgttactaag aagctgaggg gaatatgcca gcgaatcgct ttctgccata
    44881 gcaaccacac gccgccagcc agccaggcga gatttaccca ttgccaacca gcgcccgcca
    44941 gaataccgct gtagatcgga tattgcataa tctgttcaac cgaatgaccg gcacggacag
    45001 aggttttaaa tgtatccaac ggtgtcgcct gactaatgcc atcaatacct aagcgtagcg
    45061 tgttcatatc accaccactg ggggtatgtc cgctgaaaat aacctgtatg gcgtcgataa
    45121 aaccagggat gttgaccgca atttcatgtg gcggtaacca gctggtcatc tgcaccggga
    45181 aggagatcag taagaccaca taaccaatca ttgccggatt aaacgggttt tgccccagac
    45241 cgccatacaa ctgtttagcg atgatcaccg caaacaccgt acccagtacg accatccacc
    45301 atggcgcgag ggggggaata cttaccgcca gcaataagcc tgtcagcaat gctgagttat
    45361 ctttcaacgt tgcggctacc gactgcttgc gtagtttgag tacgagagct tcggctaaca
    45421 gagcactgac cgacgccaac aggatctgaa cgagagtacc ccaaccaaaa aaccacagtt
    45481 gcgctgcgat tcctggcacg gctgcgagca acaccaacag cataatgcgc gatgtctggc
    45541 gctggttatg ggtataaggg gagctagcta ttctgaatac catttagtcc tcgtttacaa
    45601 ccttttgctg ggcggctttt ttggcctgaa cgcgggcaat agccgcggca actgccgctt
    45661 tgcgcggatc aacctgttct tctggttccg cattggcctt ttgctgttcc agcttgcgtg
    45721 ctttggcgcg ggcaatagcg gcttcgacgg cggctttgcg cggatcgacc tgttcttctg
    45781 gttccgcatt agcctgttgc tgttccagct tgcgtgcttt ggcacgggca atagcggctt
    45841 cgacggcggc tttgcgcggg tcaacctttt cttctggctc cgcattagcc tgttgctgct
    45901 ccagcttgcg cgctttggcg cgggcgatag cggcttcgac ggcggctttg cgcgggtcaa
    45961 cctgttcttc tggcttcgca ttagcctgtt gctgctccag cttgcgtgct ttggcgcggg
    46021 cgatagcggc ttcaactgcg gttttacgtg gatcagcaac ggttgctgcg tcgttagttt
    46081 gctgcagttc tgcctgtttc gctctggctt gcgctttacg ggcttctcgt actgcaataa
    46141 ttgcactgtt atccgggcgt tcgcccgctt taatcacaat cggctgtgta gcctgggcct
    46201 gtttctcttt cacccgcgcc agagcggcag caatcgcatc tttatctttg gctgcaggtt
    46261 gaacggctgc gctcttatgt cgttcaaggc gagccgcttt ttcgcgctcc agacgagcct
    46321 ggcgcgcttc gaaacgcgct ttggcttctg cggcgcgctt ttcttcctga tgaatagccg
    46381 caatttcagc tttttcctga cggaaatatt gcaccagggg aatattgctc gggcaaaccc
    46441 acgcgcaagc cccacattca atgcagtcgg caatgttatg cgtggtagct ttatcgtgtt
    46501 gctgaccttt gctgaaccag tacaactgtt gcggcaaaag atccgcaggg caggcgtcag
    46561 cacaggcgct acaccggatg cagctttgtt cttcctgtgg ttcgccaagc tcattggcag
    46621 agggagccaa cagacagttg gtaatcttta cgaccgggac atccagccat ggcaaggtaa
    46681 agcccattag cgggccgccc ataatcacca tttgatcggc agaggggcag aatccggcgt
    46741 cattcaataa atgacgcact ggcgtcccca gccgtgccca gacgttgccc gggcgagcga
    46801 ttgcttcgcc agtcagggtt acgacacgct cggtaatcgg ctcgccatca ataacggcac
    46861 gtttcactgc ataagcagtg ccgacgtttt gcattaatac gccgatatcg gatgaacgcc
    46921 cgccatgtgg aacctgcttc ccggtcagaa tgtaggttaa ttgtttagca ccgccagaag
    46981 gatatttagt tggaatcacc cgcagagaaa tatcgtgaga gtccgccagc accgcgcgga
    47041 gcatggaaat cgcctgcggt ttgttatctt caatgccgat aagaatttcg cgtggctgca
    47101 gaatatgcgc aagaatgcga ataccctcta cgacctgagc cgcgcaatcc tgcatcaaac
    47161 ggtcatcggc ggtaatgtac ggctcgcact cagcagcgtt gataatcaac gtttcaatct
    47221 tatctccgcc gccctgcaat ttaacgcctg tcgggaatcc cgcaccaccc agcccggcaa
    47281 caccaaactg atgaatgcgc tcgattaact cttcgcgact gcgagagcga taatcgtccc
    47341 agccgtcgcg cgggatccag cagtcttcgc catcagcatc aataatcacg cttaattcag
    47401 ctaaagctga aggatgagcc gtagagtggg gcgcaatagc cgtaacggta cccgaggtgg
    47461 gcgcgtgaac tggcagcatt ttgccgcgac cacgggtaag cggctggccg cgcagtactt
    47521 tatcgccgac gctaacgcac aactcacctt cagagccaat atgttgtttc agtggtatga
    47581 caaaacgctg cgccaggggt acctggcgca ggggcgtacc gttggactgg gttttcatct
    47641 ccggtggatg gatgccgccg ttgaaatccc agattttatt ttttctgaat gcagagaata
    47701 acttaagcat ggtgttccac gggaatgata cgcacgggaa tggtgttcag atcccatttc
    47761 caggagtcag gtgtttctgc aaccggttgc aacgagatgc agtgcgtcgg gcacggatca
    47821 acacataaat tgcagcccgt acagagatca ctcattaccg tatgcatggc gcgggtagcg
    47881 ccaacgatgg cgtctaccgg acacgcctga atacatttag tgcagccaat acagttattt
    47941 tcatcaataa ccgccaccat ccgcgtaggc gttagctctt gcgcttcgcc atccagcggc
    48001 tgtggctcga cattaagcaa ctcagcaatt tttagcatca cagcttcgcc acctggggcg
    48061 cagcggttaa ttttttcacc gttacagctg atggcttccg cgtaggggcg acagccggga
    48121 taaccgcact gaccacactg gctctgcggt aagatttcgt caattttctc aacgaccgga
    48181 tcgtcttcca ccgcaaaacg gcgggaggca taacccagaa tggcgccaaa cgccaggccc
    48241 agcaggctca cggcggcaac ggcaatccag atagcattca ttacaacttc accaaaccac
    48301 taaagcccat aaaggccaga gacataagac ctgcggtaat taacgcaatg gcattaccgc
    48361 gaaaaggtgc cgggacatca gccacagcaa ggcgttcgcg gatggcggcg aagagcacca
    48421 tcaccagcga gaaaccgaca gcggcggaaa aaccgtacag cgccgactgc aagaaattgt
    48481 gcccgagatt gatattcagc aacgccacgc cgagcactgc acagttggtg gtgataagcg
    48541 gcaaaaaaat ccccagcaga cggtaaagca ccgggctggt tttgcgcacc accatctcgg
    48601 tgaactgcac aaccacagca atcaccagaa taaacgccag ggtgcgcagg taaatcagat
    48661 taagcgggat caaaatccac gtatcgataa gccaggcgca aatagacgcc agcgtcatca
    48721 caaacgttgt tgccagcccc atgcccatcg cggtttccag ctttttggaa actcccataa
    48781 acggacagag accgagaaac ttgaccagta caaagttatt gaccagtaca gttccgacaa
    48841 acagtaacag gtagtcagtc atttttcggc ctgaaataaa aaagccgcct attatccgtt
    48901 aatccagagc aggcgacaac aggttaactg taaggttatt acggattcac aaacgtcgct
    48961 ttcacccgcg acgagcgttt aaaatagggg acgatcaatg cggttgccaa caaagtaaac
    49021 agtaactggc gaacggctat gccatcttcc acgggtgaaa aagcgaacgc ttttactgcc
    49081 agcaatacgg aaataagcag ccagataata tagtgtttag gaacacaacg ccgacgttta
    49141 aagaaagcga tggtcaacca cagtgtgtaa taccacatgg cgatagcggt aataaaagaa
    49201 acgccccata agatttgtgt cgtcagtgct tgttcgccga gcgtttgaaa tgtttgcgga
    49261 gaagataacg cagcggtata cagcaacaac gccagcgtcg tacttaacag agcgactaaa
    49321 agccaggcca atgggccaag caaccagcct ccaatacgtt gtggcgttgt ggtggtcata
    49381 ggttctccag aatagcgacg caaacaggca aatctaattt taggggcgtt attataaggc
    49441 ttttataacc gatcgctact ttctttattg gacatagtgc cagacggact tcggtacctg
    49501 gccgaggtca aacaaacgcc cgccagaaac cagctctgcg cgacggtgat cggcagcacg
    49561 atacatattg atcagttcct gatcgtccgt cagggtgtaa ttaagatggt cgtagagttt
    49621 ttccagactt tcgagtgaac tgattttgcg aaattttaat aagtagtcct gaacagtcat
    49681 aaaaggtcca taaaataatt aataccaaag gggtaggtat tattagtgat gataaaattt
    49741 caccaaaagt gaacgcttgt gttattacaa taataagaat gcgctgatgg caaatattca
    49801 gggatgaaaa actgctctga aaagagtaac gcgctttact atctctgaca atcaccaaca
    49861 acatcgaaca agataataaa ttcctggttt aatatccgac aagtgaaaac atgcacccgg
    49921 acgggcagca tgtcgctcta caagcacaga gcttacttgt gttgtaccgt agtactctgt
    49981 tcaggtggct gatagttgtc aatgtgactc gccacgccaa gaagaatgac tgaaacgaca
    50041 agaacgatcc aacctgttaa ttcaataaga cgattcatta cagcccacat ctctgttgat
    50101 tgatccatta acttcagggg gtaaatgtta cttagcaata atagctccag caagattttt
    50161 actgaggttt ttgcgatatt tagcttttgt cgttggaaaa ttcgctattt ttttgactta
    50221 agttaaacaa catcccttat tgctggcagg ttattaaact gttgagcgag ggtaaaggat
    50281 agtgtcaaat agccatcata cttcaatgag aggcaatgac atgagcgaca acatccgtgt
    50341 tgggttgatt gggtatggtt atgcgagcaa aaccttccat gcgcccctga ttgcgggcac
    50401 gcccgggctg gaactggcgg taatctccag cagcgatgaa acaaaagtaa aagccgactg
    50461 gccaacggtt acggttgtct ctgagccgaa gcatctgttt aacgatccca acatagacct
    50521 gattgtcatc cctacaccca acgataccca tttcccgtta gccaaagcgg cgcttgaggc
    50581 gggtaaacat gtggtcgttg ataaaccctt taccgtgaca ctgtcacaag cgcgagagct
    50641 ggaagcgctg gcaaaaagcc tggggcgtgt gctgtctgta ttccataacc gtcgctggga
    50701 tagcgatttc ctgacgctaa aaggtttgct cgcggaaggc gtactgggtg aagttgctta
    50761 ctttgagtct cattttgacc gcttccgtcc gcaggtgcgc gatcgttggc gtgaacaggg
    50821 cggcccaggc agcggtatct ggtacgattt agcaccgcac cttcttgatc aggccattac
    50881 gctatttggt ttaccggtca gcatgacggt agatttggca cagttacggc ccggagcgca
    50941 gtcgaccgat tatttccacg ccatcttgtc ctatccgcag cggcgagtca ttttacaagg
    51001 taccatgctg gcagccgctg agtcagcacg ttatatcgtg catggatccc gaggcagtta
    51061 tgtaaaatat ggcctcgatc cacaggaaga acgtctgaaa aatggccagc gtctgccgca
    51121 ggaagactgg ggctacgata tgcgtgatgg cgtacttacc cgcgtggaag gtgaggaacg
    51181 tgtcgaagaa acgctgttga cagtaccagg gaattatccg gcttactatg cggctattcg
    51241 tgatgcgtta aatggcgatg gtgaaaatcc ggttccggca agtcaggcaa tccaggtaat
    51301 ggagttgatt gagctgggca tcgaatccgc caaacatcgc gcgacgctgt gccttgcgtg
    51361 aaaagaggat ggtgcaatta gcaccatcca tttttgttac ttcgcagcga ctttttctcg
    51421 cagtgcgcgt ttttcctctg cgctgaggaa agccatttcc agaccattaa tctgcgcctg
    51481 gcggatttgc tcgcgggata acccagcagc cggcgcggca acggtatatt cgtgaatgat
    51541 atccactccc tgtacgccgg gatcgtcagt gttaatgctg gcacgaatgc catgctcaag
    51601 gaacgttttc agcggatgtg ccgccagctc tgccacggtg ctggtctgaa tattggaggt
    51661 cagacaggat tcaataccaa tttgctgctc ggcgagaaaa tccatcagcg cccgatcttc
    51721 aatggctttt acgccatgtc caatacgttc cgcccccagt tcacgaatcg cctgccagat
    51781 gctttccggc ccggcagctt cgcctgcatg gacggtaata tgccagcccg catcacgcgc
    51841 gcggttgaag tgagaaagga acagacttcc cgggaaacca agttcatcac cggctaaatc
    51901 aagtgcggta atctggtctc ggtgggctaa aaaggcctcc agctcttgct gacaggcggc
    51961 ttcaccgaag gtccggctca taatgccgat aagcttcgcc tgtacaccaa aggtgcggca
    52021 accctcacgt acgccatcga tcaccgcttc gacaacaccc gctacaggca gctgatgtgc
    52081 cattgccatg tagcccggtg aaaaacgcag ctcgacatag tgcaggccgt tacgggctgc
    52141 atcttcaatg ttttcaaatg acacgcggcg acaggcatca agagaggcga gaactttaac
    52201 gccccagtca agtttggtca gaaagctcac aagatcgggt tcgttggcaa tgacctgaac
    52261 gtggggaatc agtgtttcta gggattgtgc aggaagcgag atattatact ggcggccaag
    52321 ttcaagaatg gtctggggac gaatgttgcc atcaaggtgg cgatggatat cagttaatgg
    52381 cagggtggta tcaatcatgg tcgcactctt tttctggata taaaagtgcg caccattata
    52441 aaaacaaagc agggtaaaaa tccacgattg cgcaacgatt ttttacgttg cgcaattggg
    52501 gttaacgaac agcgcggatg gcgttaatta atccagcaac acctttttcc agtttcgaac
    52561 gcgggcaccc ggcattgaga cggacaaaac cacgaccttc ttcaccgtag gtataccccg
    52621 gcatgatcgc gaccttctct tgctcgataa gcgctttttg caacgcgttg tcgtcaatat
    52681 tcaacggacg tagatcaatc caggccagat aagtggattg cgggatctgc cagttgagtt
    52741 cagggaacgc ggcgttcatt ttatctgcga tatacgtcag gttatctttc agatagacgc
    52801 gtaaggcatc cagccacggc gcgccttgct gataggcggc gatatgggca gttaacgcca
    52861 gtactgaagg ggaagaaagg ccatcacgtc ctttcagtgc cgataaatag gcatcacggc
    52921 tgctgctatt ttctataatc ccgtaagcac cggtcagggc gggaatattg aaacttttcg
    52981 atcccgacgt tagcaatgcc cagtctccgc gcgccacatt actccaggga atatgcggct
    53041 gctcggccca aaccatatcc atatggattt catcggaaat aacccgcaca ccatgacgct
    53101 cgcacaggtc agccatgatc tccagttcat cgcacgtcca cactttcccg gtaggattct
    53161 gtgggctaca caggagcata attttacatt ctggtttcgc caacacggct tcaagcttgc
    53221 ccatatcgca aaaccagccg tcagcccgct tctctaaagc gacgggcatt actgtgcgct
    53281 ggttaccttc aatggccttg taaaatgcgt cataggcggg tgtgtggatc accacgcctt
    53341 cacctgtttc agaccactga cgaatcagtt ctgaaaccat atagataaca gaagggccat
    53401 acaccaccga ctgcgtatca attgcggtgt aatgctgggt ggaaaaccag tgggcaatag
    53461 ccgcgagaaa ctcatcgttt ttccagcggc tgtagccaaa cacgccgtgc atcaggcgct
    53521 gattcagcgc ctcgataatg cagggggcag tggcaaaatc catgtctgaa atcgtgaacg
    53581 gtaacaggtc agcagtgccg aaacggtcag cgacataatc ccactgcgta caccatgtgc
    53641 catgacgatc cacgaccttt gaaaaatcga acatatctta tccttatgcc tggacagtat
    53701 gcatcagacc ggccatttca tcttttactg actgaacttg tggcccgata acaacctgca
    53761 ggttatgttg attaagttgt actacgccaa ttgcccgatt gtccttcagt gcctgcacat
    53821 taacaagcga catatctttc acagacaaac gcagacgggt aatgcagtta tcgaggctga
    53881 caatattgtc ggcaccgcct aaggcttcga ggattgccgg aacgttgtaa cctgatttac
    53941 ccggcacacc ggcaacggct ttttcgattg agctggcaac ttcgctatcg cgccccgggg
    54001 ttttcagatt gaagcgggtg atagcgaaac ggaagatgac gtagtaaacg acaaaccaga
    54061 ttgccgccac cactggcacc atgtaccact tggttgacag accatgcaaa ataccgaaca
    54121 ccacgaagtc gatgatattg ccgtcggtat taccgatggt gacgccgagc acagacatga
    54181 cggtgaagcc gaggccggtt aacagcgcgt ggatgacata cagaactggc gctacgaaca
    54241 ggaacaggaa ttccagcggt tcggtcgtgc cgccaacgac gcaggcgatc aggccagaaa
    54301 tcagcagacc tttaatttta tggcgatttt ccgggcgcgc gcagtgatac atagctaacg
    54361 ctgcacctgg cagaccgccg agaaacgcag gcattttacc ttgcgaaagg aaacgcgtgg
    54421 cgctttcaga aaaaccgtga gtggtcgggc aactcaattg cgcctggaag atggtcagtg
    54481 cgccgctgac ggtttgaccg cagacttcct gcgtgccgcc tgcgtcggta aagcgaatta
    54541 atgccaccag aatgtgatgc agaccaaacg gcaacagcag acgttcaccg gtaccaaaca
    54601 gcatcggtcc gaaatcaccc gcactgttga tcatatggcc caggccgcta atacccatgg
    54661 cgaaaatcgg ccagactaat ggaatcacca gaccgacaag gcccatcacc agcgaggaga
    54721 tgattggtac gaagcgcgta ccgccgaaga atgccagcgc atccggcagg cggatattat
    54781 ggaatcgctc atgcagcatc cagacgataa taccggcgat caccgcaccg aggatcccgg
    54841 tatcgatcga ctggatccca agaatgctct gtatgttatt ggttttcaga acggcggcgt
    54901 ctgtggttgg cagaatgcct ttattggtta accagaagtt taccgcgagg ttcattaccg
    54961 cataaccgac gaagccagcg aatgccgcta cgcctttgtt ttcgcgtgcc aggcccagcg
    55021 ggatggcgat acagaacatg actggcagga aactaaaagc aaacgagcca atcttactca
    55081 tccaggtaaa gatagcctgc aacacggggt tgcccaggac cgggattagg gtgatgacat
    55141 cgtggctact aagggaacta ccaatgccga gcataatgcc gcagaacgac aataatgcca
    55201 cgggtaacat gaaggttttg cctaactgct ggaagaactc ccacagcgtg actttcggtg
    55261 ctgttttcgc cgtcataaaa cgactcctcg tagagaacaa tctggattca gtaaattgcg
    55321 cgaaaagata aaacgtttta tcaaatttta gtgaggcata aatcacatta cgcaacgata
    55381 atagcgggta taagataaat aaaaggtaaa acgttttatc tgtcacataa tcagggagta
    55441 ggtcatctgc atggctaccg ccaaaaaaat aaccattcat gatgttgcgc tggctgcggg
    55501 cgtgtcggta agtaccgttt cgctggtgct tagtggcaaa gggcgaatct ctaccgccac
    55561 aggagaacgc gttaacgccg ccattgaaga gctgggattt gtgcgcaatc gccaggcgtc
    55621 ggcgctgcgc ggcgggcaaa gcggcgtcat tggtttgatc gtccgtgatt tatctgcgcc
    55681 gttttacgcc gaattgacgg ccggattgac ggaagctctg gaagcgcagg gacggatggt
    55741 gtttttgctt cacggcggta aagacggcga gcagctggca caacggtttt cactgttact
    55801 gaatcagggg gtcgatggtg tggtaattgc cggggctgca ggaagcagcg atgacctgcg
    55861 acggatggca gaagaaaaag ctatcccggt gattttcgct tcccgtgcca gttatcttga
    55921 tgatgttgat acggttcgcc cggacaacat gcaggctgca cagttgttga cggagcatct
    55981 cattcgcaat gggcatcagc ggatcgcctg gctgggaggg caaagttcct cattaacccg
    56041 tgcagaacgg gtggggggct attgtgcgac tctactaaaa tttggcctgc cgtttcacag
    56101 tgattgggtg ctggagtgca cttccagcca gaagcaagcc gcggaagcta tcacggcgct
    56161 tttacgtcat aacccgacca tcagtgccgt ggtttgctat aacgaaacta ttgcgatggg
    56221 ggcatggttt ggtttgctga aagcagggcg gcaaagcggg gaaagcggag tcgatcgtta
    56281 ctttgagcaa caggtttcgc tggcggcatt taccgatgcg acaccaacca cacttgatga
    56341 tatacccgtt acctgggcca gcacgccagc gcgggaactt ggtaccacac ttgcggatcg
    56401 catgatgcaa aaaatcactc atgaagaggc gcattcacgc aatcttatta ttcccgcccg
    56461 gctcattgcg ccgaaataat cctctcttta tctgctatac ctggtagtgt cctttcctca
    56521 aggttaatga taaaaaatgt ggcacacagg cggtgccttt tatttttact gaagttcaca
    56581 ggaggtttat gtgtttaatt ctgacaacct gagactcgac ggaaaatgtg ccatcatcac
    56641 aggtgccggt gcaggtattg gtaaagaaat tgccattaca ttcgcgacag ctggcgcatc
    56701 tgtggtggtc agtgatatta acgccgatgc agctaatcat gttgtagatg aaattcaaca
    56761 actgggtggt caggcatttg cctggcgctg cgatattact tccgaacagg aactctctgc
    56821 actggcagac tttgccgtca gtaagctggg taaagttgat atcctggtta acaacgccgg
    56881 tggcggtggg cctaaaccgt ttgatatgcc aatggcagat tttcgccgcg cttatgaact
    56941 gaatgtattt tcttttttcc atctgtcaca acttgttgcg ccagaaatgg aaaaaaatgg
    57001 cggtggcgtt attctgacca ttacttctat ggcggcagaa aataaaaata taaacatgac
    57061 ctcctatgca tcatctaaag ctgcggccag tcatctggtc agaaacatgg cgtttgacct
    57121 gggtgaaaaa aatattcggg tgaatggcat tgcgccgggg gcaatattaa ccgatgccct
    57181 gaaatccgtt attacaccag aaattgaaca aaaaatgtta cagcacacgc cgatcagacg
    57241 tctgggccaa ccgcaagata ttgctaacgc ggcgctgttc ctttgctcgc ctgcagccag
    57301 ctgggtaagc ggacaaattc tcaccgtctc cggtggtggg gtacaggagc tcaattaata
    57361 cactaacgga ccggtaaaca accgtgcgtg ttgtttaccg ggataaactc atcaacgtct
    57421 cagctaaata actggcagcc aaatcacggc tattggttaa ccaatttccg agtgaaaagt
    57481 atacgaatag agtgtgcctt cgcactattc aacagcaatg ataggcgctc acctgacaac
    57541 gcggtaaact agttattcac gctaactata atggttttat gatggataac atgcagactg
    57601 aagcacaacc gacacggacc cggatcctca atgctgccag agagattttt tcagaaaatg
    57661 gatttcacag tgcctcgatg aaagccatct gtaaatcttg cgccattagt cccgggacgc
    57721 tctatcacca tttcatctcc aaagaagccc tgattcaggc gattatctta caggaccagg
    57781 agagggcgct ggcccgtttc cgggaaccga ttgaagggat tcatttcgtt gactatatgg
    57841 tcgagtctat tgtctctctc acccatgaag cctttggaca acgggcgctg gtggtcgaaa
    57901 ttatggcgga agggatgcgt aacccacagg tcgccgccat gcttaaaaat aagcatatga
    57961 cgatcacgga atttgttgcc cagcggatgc gtgatgccca gcaaaaaggc gagataagcc
    58021 cagacatcaa cacggcaatg acttcacgtt tactgctgga tctgacctac ggtgtactgg
    58081 ccgatatcga agcggaagat ctggcgcgtg aagcgtcgtt tgctcaggga ttacgcgcga
    58141 tgattggcgg tatcttaacc gcatcctgat cctctctctc tttccgcggg ctggtgacaa
    58201 ctgagcccgc gtttcatatc gtaatttctc tgtgcaaaaa ttatccttcc tgggttcgga
    58261 gaattccccc caaatattca ctgtagccat atgtcatgag agtttatcgt tcccaatatg
    58321 ctcgaacgaa cgttcgattg cttattttgt ggcttatgtc aacgctgttt taaagattaa
    58381 tgcgatctat atcacgctgt gggtagtgca gtttttggtt ttttgatcgc ggtgtcagtt
    58441 ctttttattt ccatttctct tccatgggtt tctcacagat aactgtgtgc aacacagaat
    58501 tggttaacta atcagattaa aggttgacca atatttttat cttaatgagg agtcccttat
    58561 gttacgtcct gtagaaaccc caacccgtga aatcaaaaaa ctcgacggcc tgtgggcatt
    58621 cagtctggat cgcgaaaact gtggaattga tcagctttgg tgggaaagcg cgttacaaga
    58681 aagccgggca attgctgtgc cgggcagttt taacgatcag ttcgccgatg cagatattcg
    58741 taattatgtg ggcaacgtct ggtatcagcg tgaagtcttt ataccgaaag gttgggcagg
    58801 ccagcgtatc gtgctgcgtt tcgatgcggt cactcattac ggcaaagtgt gggtaaataa
    58861 tcaggaagtg atggagcatc agggcggcta tacgccattt gaagccgatg tcacgccgta
    58921 tgttattgcc gggaaaagtg tacgtatcac cgtttgtgtg aacaatgaac tgaactggca
    58981 gactatcccg ccgggaatgg tgattaccga tgaaaacggc aagaaaaagc agtcttactt
    59041 ccatgatttc tttaactatg ccgggatcca tcgcagcgta atgctctaca ccacgccgaa
    59101 cacctgggtg gacgatatca ccgtggtgac gcatgtcgcg caagactgta accacgcgtc
    59161 tgttgactgg caggtggtgg ccaatggtga tgtcagcgtt gaactgcgtg atgcggatca
    59221 acaggtggtg gcaactggac aaggcaccag cgggactttg caagtggtga atccgcacct
    59281 ctggcaaccg ggtgaaggtt atctctatga actatgcgtc acagccaaaa gccagacaga
    59341 gtgtgatttc tacccgctgc gcgtcggcat ccggtcagta gcagtgaagg gcgaacagtt
    59401 cctgatcaac cacaaaccgt tctactttac tggctttggt cgtcatgaag atgcggattt
    59461 gcgcggcaaa ggattcgata acgtgttgat ggtgcacgat cacgcattaa tggactggat
    59521 tggggccaac tcctaccgta cctcgcatta cccttacgct gaagagatgc tcgactgggc
    59581 agatgaacat ggcatcgtgg tgattgatga aactgcagct gtaggcttta acctctcttt
    59641 aggcattggt ttcgaagcgg gcaacaagcc gaaagaactg tatagcgaag aggcagtcaa
    59701 cggagaaacc cagcaggcgc acttacaggc gattaaagag ctgattgcgc gtgacaaaaa
    59761 ccacccaagc gtggtgatgt ggagtattgc caacgaaccg gatacccgtc cgcaaggtgc
    59821 acgggaatat ttcgcgccac tggcggaagc aacgcgtaaa ctcgacccga cgcgtccgat
    59881 cacctgtgtc aatgtaatgt tctgcgacgc tcacaccgat accatcagcg atctctttga
    59941 tgtgctgtgc ctgaaccgtt attacggttg gtatgtccaa agcggcgatt tggaaacggc
    60001 agagaaggta ctggaaaaag aactgctggc ctggcaggag aaactgcatc agccgattat
    60061 catcaccgaa tacggcgtgg atacgttagc cgggctgcac tcaatgtaca ccgacatgtg
    60121 gagtgaagag tatcagtgtg catggctgga tatgtatcac cgcgtctttg atcgcgtcag
    60181 cgccgtcgtc ggtgaacagg tatggaattt cgccgatttt gcgacctcgc aaggcatatt
    60241 gcgcgttggc ggtaacaaga aggggatctt cacccgcgac cgcaaaccga agtcggcggc
    60301 ttttctgctg caaaaacgct ggactggcat gaacttcggt gaaaaaccgc agcagggagg
    60361 caaacaatga atcaacaact ctcctggcgc accatcgtcg gctacagcct cggtgacgtc
    60421 gccaataact tcgccttcgc aatgggggcg ctcttcctgt tgagttacta caccgacgtc
    60481 gctggcgtcg gtgccgctgc ggcgggcacc atgctgttac tggtgcgggt attcgatgcc
    60541 ttcgccgacg tctttgccgg acgagtggtg gacagtgtga atacccgctg gggaaaattc
    60601 cgcccgtttt tactctttgg tactgcgccg ttaatgatct tcagcgtgct ggtattctgg
    60661 gtaccgaccg actggagcca tagcagcaaa gtagtgtatg catatttgac ctacatgggc
    60721 ctcgggcttt gctacagcct ggtgaatatt ccttatggtt cacttgctac cgcgatgacc
    60781 caacaaccac aatcccgcgc ccgtctgggc gcggctcgcg ggattgccgc ttcattgacc
    60841 tttgtctgtc tggcatttct gataggaccg agcattaaga actccagtcc ggaagagatg
    60901 gtgtcggtat accatttctg gacgattgtg ctggcgattg ccggaatggt gctttacttc
    60961 atctgcttca aatcgacgcg tgagaatgtg gtacgtatcg tggcgcagcc gtcattgaag
    61021 atcagtctgc aaaccctgaa acggaatcgc ccgctgttta tgttgtgcat cggtgcgctg
    61081 tgtgtgctga tttcgacctt cgcggtcagc gcctcgtcgt tgttctacgt gcgctatgtg
    61141 ttaaatgata ccgggctgtt cactgtgctg gtactggtac aaaacctggt cggtactgtg
    61201 gcatcggcac cgctggtgcc ggggatggtc gcgaggatcg gtaaaaagaa taccttcctg
    61261 attggcgctt tgctgggaac ctgcggttat ctgctgttct tttgggtttc ggtctggtca
    61321 ctgccggtgg cgttggttgc gttggccatc gcttcaattg gtcagggcgt taccatgacc
    61381 gtgatgtggg cactggaagc tgatactgta gaatacggtg aatacctgac cggcgtgcga
    61441 atagaagggc tcacctattc actattctca tttacccgta aatgcggtca ggcaatcgga
    61501 ggttcaattc ctgcctttat tttgggatta agcggatata ttgccaatca ggtgcaaacg
    61561 ccggaagtta ttatgggcat ccgcacatca attgccttag taccttgcgg atttatgctg
    61621 ctggcattcg tcattatctg gttttatccg ctcacggata aaaaattcaa agaaatcgtg
    61681 gttgaaattg ataatcgtaa aaaaatgcag cagcaattaa tcagcgatat cactaattaa
    61741 tattcaataa aaataatcag aacatcaaag gtgcaactat gagaaaaata gtggccatgg
    61801 ccgttatttg cctgacggct gcctctggcc ttacctctgc ttacgcggca caactggctg
    61861 acgatgaagc gggactacgc atcagactga aaaatgaatt acgcagggct gataagccca
    61921 gtgctggcgc gggaagagat atttacgcat gggtacaggg aggattgctc gatttcaata
    61981 gtggttatta ttccaatatt attggcgttg aaggtggggc gtattatgtt tataaattag
    62041 gtgctcgtgc tgatatgagt acccggtggt atcttgatgg tgataaaagt tttggctttg
    62101 ccctgggagc agtaaaaata aaacccagtg aaaatagcct acttaaatta ggtcgcttcg
    62161 ggacggatta tagttatggt agcttacctt atcgtattcc gttaatggct ggcagttcgc
    62221 aacgtacatt accgacagtt tctgaaggag cattaggtta ttgggcttta acgccaaata
    62281 ttgatctgtg gggaatgtgg cgttcacgag tatttttatg gaccgattca acaaccggta
    62341 ttcgtgatga aggggtgtat aacagccaga cgggaaaata cgataaacac cgcgcacgtt
    62401 cttttttagc cgccagttgg catgatgata ccagtcgcta ttctctgggg gcatcggtac
    62461 agaaagatgt atccaatcag atacaaagta ttctcgagaa aagcataccg cttgacccga
    62521 attatacgtt gaaaggggag ttgctcggct tttacgcgca actcgaaggt ttaagtcgta
    62581 ataccagcca acccaatgaa acggcgttgg ttagtggaca attgacctgg aatgcgccgt
    62641 ggggaagcgt atttggcagt ggtggttatt tgcgccatgc aatgaatggt gccgtggtgg
    62701 ataccgacat tggctatccc ttttcattaa gtcttgatcg taaccgtgaa ggaatgcagt
    62761 cctggcaatt gggtgtcaac tatcgtttaa cgccgcaatt tacgctgaca tttgcaccga
    62821 ttgtgactcg cggctatgaa tccagtaaac gagatgtgcg gattgaaggc gcgggtatct
    62881 taggtggtat gaactatcgg gtcagcgaag ggccgttaca agggatgaat ttctttcttg
    62941 ctgccgataa aggacgggaa aaacgcgatg gcagtgcgct gggcgatcgc ctgaattact
    63001 gggatgtgaa aatgagtatt cagtatgact ttatgctgaa gtaacaaata acgccggaga
    63061 gaaaaatctc cggcgttttg gctgtgaatt actgctgcgg aatagcgggt acagccggaa
    63121 cgtttaatgc cggcattgca aacatcccaa caaaatcttc cagtggcatt ttctgcccgt
    63181 ttaacgtgat ctgaccgtta gcatattgca ggctggtggt gatggtattg tcctgcaagg
    63241 tggtcaggcg gaacatctgc cccattgctg acgcaccttc aacttgctgt ttcgccagtt
    63301 tttttgcttg atcttcctga taaccttcca gcttcgctac ctgagtcata aactcagttg
    63361 ccatatccac cggaatggtc agtttcgcat ccagagattt aaccgagcgg tctacttcct
    63421 gcgccagcgt ttgcggcgct tctttagtcg ttgccggatc tttcaggaac agcgacagat
    63481 tcagcgcact ttcaccctga ctgtttttcc agcttagcgg cgcgatagta atcaccggat
    63541 cgcctttcaa catcagcggc agggcgctaa agaaggcttc cgtcactttc tcctgataaa
    63601 gttcgggatt gttggcaatt tctggctgtg ccagcagcgc ctgagtttgc gcgttatatt
    63661 gctggctaaa ctgatgccag gcttcaccat caatctggcc gacttttaaa gtcagcttgc
    63721 cgctgcccag atcctgattc tgtaccttca ggctgtttag cgagtaatcc agttggctat
    63781 tgatcgtttt accgtcattg accagatccg atttaccgct gatctccatg ccttccagca
    63841 gtgccagttc tttgccttcc actgaaatgg tcattttttc cagcgacagt ttttgatttc
    63901 ctacgcgctc accaaaactt gccagcgtgc tggaaccgtc ggttttcaga ttattaaagg
    63961 tcaactgtac tttctggttg tattcgttaa ccgcgtctat ccgaccactt tgcgcctccc
    64021 cggaaaggga gatagctttg ccgtctctgt ctgcatttaa ctggaactcg ccgccgctaa
    64081 aggcgacttt ttcatccttt tgctcgtaat tcagtggctt gagcgaaata tcggaactgg
    64141 aatcacctct gtaaccaatg cgggagttaa tctcaaaagg cgtttcacct tttgccatat
    64201 caaacagtgg cttgctaact tcgttattaa ccagcgtggt ttgaattgat gccatcgacg
    64261 ggatcaggtt cagttttttt agctgggcaa gggggaaggg accatgatca actgattcgt
    64321 tgaagatgac gctctgaccg cttttaatcc acggattttc tttcccggca atgggtttca
    64381 ccaatagttg cagctgactg ctgaatacgc cgcgatgata gttttgataa ctcacttcca
    64441 ggttggattc aggagcggtc agtttgagtt gcgcgttcgc ctgcgcgacc atgtcttcga
    64501 gatgggtttc aatcttcttg cctgtatacc atgcgccgcc tgtccagact acgcctagcg
    64561 caacaatgac gcctaccgct accagcgatt tattcataat gattatccat aaaatgaaat
    64621 caggcggact ggccgcctga aggtgttata agcctttaat aagcttagca agagatgtta
    64681 attttttcag taagctctta cagcttgttg taaacacgcg ctaaacggcc gtggcctttg
    64741 acagtcaccg gtgattcgtt ggcggcaata aacgctgatt cacccggttt aagctgtaac
    64801 tgctgagaac ctttccacag cgttgcatcg ccttcgacgc agaacaaaat ggcggcactc
    64861 tgctggctga tggtggtttc tttatcacta aggtcatgca gcgagaaggc aaaatcatcc
    64921 acaggaatcg ggaagtccag ttctgcacct tgtttcaccg gctgggtcaa caactggtta
    64981 gccggtttgg cttcgaactt cacattggca accagttccg gaatatcaat gtatttaggc
    65041 gtcagacccg cacgcagcac gttatcggag ttcgccatca cttccagcgc cacgccttgc
    65101 aggtaagcgt gcggtgtttc agcgaacagg aacatcgctt cgccaggatt caatttcacc
    65161 acattcagca acagcgggga gaacagaccg ctgtcatccg ggtaaaattc agaaattaaa
    65221 cgaattgttt gccacggttc accctgctgg ctgtcaaggg ccgattttaa aatcgccagc
    65281 gcgcgggatt tttcttcacc ctgcatattc aacaggctgg cgaacagttc gcttaaacgt
    65341 tcggcatcag gctgttgtaa aaagtgagca atcgccggat gtgcacctgc gaccggctgg
    65401 agcagggaga caatctcgga aaactcacga aacgcgttca tcgcaaggaa aggcgtcagc
    65461 gcaaaaacca gctccggctt gtggttagga tctttatagt tacgctcggc ggcatccaac
    65521 ggaatacctg cggcattttc tttggcaaaa ccgatttcag aattgcgttt gtttggatga
    65581 acctgaatgg agagcggctg tgctgcgcat aatactttga acaggaaagg cagttcgcca
    65641 aagcgtttgg caacggcctc tccgagcaga gtcgatttat cactctcaat cacatcacgc
    65701 agtgaaacga tatctccggc ggcattctgc actcgtgaac tgcttttcgg atgtgcgccc
    65761 atccacagct cggccatcgg ctggctggac ggattttcca taccataaag ttcagtcaac
    65821 gccgttttgc tgccccaggc atagttttgc actgagttaa tgagtttttg catgatcaat
    65881 ccctgtttta atgtggaaat taatcccgct attaaagcaa gaatcctacg ggaagtaacc
    65941 tggagccgca aaaagtcgta ctagtctcag tttttgttaa aaaagtgtgt aggatattgt
    66001 tactcgcttt taacagggca acggaacacc cgcccagagc ataaccaaac caggcagtaa
    66061 gtgagagaac aatgtcaaac aaaccctttc attatcaggc tccttttcca ctcaaaaaag
    66121 atgatactga gtattacctg ctaaccagcg aacacgttag cgtatctgaa tttgaagggc
    66181 aggagatttt gaaagtcgca cccgaagcgt taactttgtt ggcgcgtcag gcgtttcatg
    66241 atgcgtcatt tatgctgcgt ccggctcacc aacaacaggt ggccgacatt ctgcgtgacc
    66301 cggaagccag cgaaaatgat aaatatgtgg cgctgcaatt cctgcgcaac tccgacatcg
    66361 ccgcaaaagg cgttctgcca acctgtcagg ataccggcac cgcgattatt gttggtaaaa
    66421 aagggcagcg tgtatggacc ggtggcggtg atgaagcggc gctggcacgc ggtgtctata
    66481 acacttatat cgaagataat ctgcgctact cgcaaaacgc gccgctggat atgtataaag
    66541 aggtgaatac cggcaccaat ctaccagcgc agatcgatct ttatgccgtt gatggcgacg
    66601 agtacaaatt cctctgtatc gccaaaggtg gcggttcggc aaacaagacg tatctctatc
    66661 aggaaaccaa agcgttactg acgccgggga aactgaaaaa ttacctggtt gagaagatgc
    66721 gcacgctggg tacggcggcc tgtcctccgt atcatattgc gttcgttatt ggtggaactt
    66781 ctgcagaaac gaaccttaaa acggtgaaac tggcttccgc taaatactat gatgaactgc
    66841 caacggaagg gaatgagcac ggacaggcgt tccgcgatgt ggaactggaa aaagaattgc
    66901 tgatcgaagc gcaaaatctt ggtctgggtg cgcagtttgg cggtaaatac ttcgctcacg
    66961 acatccgcgt gattcgcctg ccacgtcacg gcgcatcctg cccggtcggt atgggcgtct
    67021 cctgttctgc tgaccgtaat atcaaagcaa agatcaaccg tcaggggatt tggatcgaaa
    67081 aactggaaca taatccaggc aaatatatcc cggaagagct gcgcaaagcg ggagaaggcg
    67141 aagcggtgcg cgttgacctt aaccgtccga tgaaagagat cctcgcacag ttgtcgcagt
    67201 atcccgtttc tacacgctta tcgctgaatg gcacgattat cgttggtcgt gatattgctc
    67261 acgccaaact gaaagagcgg atggataacg gcgaagggct gccgcagtat attaaagatc
    67321 atccgattta ctacgcgggt ccggccaaaa cgccggaagg ttatgcctcc ggttctcttg
    67381 gcccaacgac cgccggacgg atggattctt atgtcgatca actgcaagcg cagggcggaa
    67441 gtatgatcat gctggcgaaa ggcaaccgca gccagcaggt gacggatgcc tgtaaaaaac
    67501 acggcggttt ctacctcggt agtatcggtg gtccggcagc tgtattggcg cagggaagta
    67561 ttaagagcct ggaatgtgtt gaatatccgg aactgggaat ggaagccatc tggaaaattg
    67621 aagtggaaga tttcccggca tttatccttg tggatgataa aggaaatgac ttcttccagc
    67681 agatacaact cacacagtgc acccgctgtg tgaaataaac agagccgccc ttcggggcgg
    67741 tttttttaca tggcacgaaa gaccaa
//
LOCUS       DACBVI010000004        67544 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.004, whole genome shotgun sequence.
ACCESSION   DACBVI010000004 DACBVI010000000
VERSION     DACBVI010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.004"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..953
                     /gene="ldtC"
                     /locus_tag="IU325_01330"
     CDS             <1..953
                     /gene="ldtC"
                     /locus_tag="IU325_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HAP5807676.1"
                     /translation="THFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGENKFHV
                     VENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPREGIVI
                     NIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTANIRAR
                     YKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCIRLRD
                     DDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPITLNS
                     AMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(1036..1293)
                     /gene="bhsA"
                     /locus_tag="IU325_01335"
     CDS             complement(1036..1293)
                     /gene="bhsA"
                     /locus_tag="IU325_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HAP5807677.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            1534..2166
                     /gene="comR"
                     /locus_tag="IU325_01340"
     CDS             1534..2166
                     /gene="comR"
                     /locus_tag="IU325_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HAP5807678.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDHFAAKHEAQLFCEEKSVETALADYFA
                     AVANCFTSKDTPAGCFMINNCTTLSPDSGDIGNTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIARTTLRLWPELVK"
     gene            complement(2228..2767)
                     /locus_tag="IU325_01345"
     CDS             complement(2228..2767)
                     /locus_tag="IU325_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAP5807679.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDGNGQLILNNKV"
     gene            complement(2994..4298)
                     /locus_tag="IU325_01350"
     CDS             complement(2994..4298)
                     /locus_tag="IU325_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP5807680.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(4629..5171)
                     /locus_tag="IU325_01355"
     CDS             complement(4629..5171)
                     /locus_tag="IU325_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5807681.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(5194..6219)
                     /gene="nagZ"
                     /locus_tag="IU325_01360"
     CDS             complement(5194..6219)
                     /gene="nagZ"
                     /locus_tag="IU325_01360"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAP5807682.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVCQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(6230..7054)
                     /gene="thiK"
                     /locus_tag="IU325_01365"
     CDS             complement(6230..7054)
                     /gene="thiK"
                     /locus_tag="IU325_01365"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HAP5807683.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRRLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(7035..7676)
                     /gene="lpoB"
                     /locus_tag="IU325_01370"
     CDS             complement(7035..7676)
                     /gene="lpoB"
                     /locus_tag="IU325_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HAP5807684.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(7690..8067)
                     /locus_tag="IU325_01375"
     CDS             complement(7690..8067)
                     /locus_tag="IU325_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1425 domain-containing protein"
                     /protein_id="HAP5807685.1"
                     /translation="MRKGCFGLVSLALLVLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(8070..8429)
                     /gene="hinT"
                     /locus_tag="IU325_01380"
     CDS             complement(8070..8429)
                     /gene="hinT"
                     /locus_tag="IU325_01380"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HAP5807686.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            8763..10952
                     /gene="fhuE"
                     /locus_tag="IU325_01385"
     CDS             8763..10952
                     /gene="fhuE"
                     /locus_tag="IU325_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HAP5807687.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIVLALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(11012..12445)
                     /gene="ptsG"
                     /locus_tag="IU325_01390"
     CDS             complement(11012..12445)
                     /gene="ptsG"
                     /locus_tag="IU325_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HAP5807688.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(12739..13536)
                     /locus_tag="IU325_01395"
     CDS             complement(12739..13536)
                     /locus_tag="IU325_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP5807689.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(13547..14551)
                     /gene="holB"
                     /locus_tag="IU325_01400"
     CDS             complement(13547..14551)
                     /gene="holB"
                     /locus_tag="IU325_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP5807690.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVQSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHYGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(14548..15189)
                     /locus_tag="IU325_01405"
     CDS             complement(14548..15189)
                     /locus_tag="IU325_01405"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP5807691.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTNWVKELDA"
     gene            complement(15179..16201)
                     /gene="yceG"
                     /locus_tag="IU325_01410"
     CDS             complement(15179..16201)
                     /gene="yceG"
                     /locus_tag="IU325_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HAP5807692.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVSSERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(16204..17013)
                     /gene="pabC"
                     /locus_tag="IU325_01415"
     CDS             complement(16204..17013)
                     /gene="pabC"
                     /locus_tag="IU325_01415"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HAP5807693.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLELEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVIPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(17133..18374)
                     /gene="fabF"
                     /locus_tag="IU325_01420"
     CDS             complement(17133..18374)
                     /gene="fabF"
                     /locus_tag="IU325_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP5807694.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(18462..18698)
                     /gene="acpP"
                     /locus_tag="IU325_01425"
     CDS             complement(18462..18698)
                     /gene="acpP"
                     /locus_tag="IU325_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP5807695.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(18909..19643)
                     /gene="fabG"
                     /locus_tag="IU325_01430"
     CDS             complement(18909..19643)
                     /gene="fabG"
                     /locus_tag="IU325_01430"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HAP5807696.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(19656..20585)
                     /gene="fabD"
                     /locus_tag="IU325_01435"
     CDS             complement(19656..20585)
                     /gene="fabD"
                     /locus_tag="IU325_01435"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP5807697.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(20601..21554)
                     /gene="fabH"
                     /locus_tag="IU325_01440"
     CDS             complement(20601..21554)
                     /gene="fabH"
                     /locus_tag="IU325_01440"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HAP5807698.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLTANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(21622..22692)
                     /gene="plsX"
                     /locus_tag="IU325_01445"
     CDS             complement(21622..22692)
                     /gene="plsX"
                     /locus_tag="IU325_01445"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP5807699.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(22773..22946)
                     /gene="rpmF"
                     /locus_tag="IU325_01450"
     CDS             complement(22773..22946)
                     /gene="rpmF"
                     /locus_tag="IU325_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP5807700.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(22998..23519)
                     /gene="yceD"
                     /locus_tag="IU325_01455"
     CDS             complement(22998..23519)
                     /gene="yceD"
                     /locus_tag="IU325_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HAP5807701.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            23718..24302
                     /locus_tag="IU325_01460"
     CDS             23718..24302
                     /locus_tag="IU325_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP5807702.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(24414..25373)
                     /gene="rluC"
                     /locus_tag="IU325_01465"
     CDS             complement(24414..25373)
                     /gene="rluC"
                     /locus_tag="IU325_01465"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HAP5807703.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDDGLKRCLQKLRNAR"
     gene            25946..29131
                     /locus_tag="IU325_01470"
     CDS             25946..29131
                     /locus_tag="IU325_01470"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HAP5807704.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAATATPASPA
                     QPGLLSRFFGALKALFSGGEETKPAEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPAVSAPVVEEVAEVVEAPVQVAEPQPEVVETTH
                     PEVIAAAVTEHPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVKPEVTVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            29264..29557
                     /locus_tag="IU325_01475"
     CDS             29264..29557
                     /locus_tag="IU325_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAP5807705.1"
                     /translation="MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEA
                     ARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPNAALRKTMQTPAPWEQKK"
     gene            29554..30048
                     /locus_tag="IU325_01480"
     CDS             29554..30048
                     /locus_tag="IU325_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP5807706.1"
                     /translation="MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTF
                     VSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEPIPVVVLGRLAIDKSLQGQGIGR
                     AMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
                     ECLK"
     gene            complement(30143..31096)
                     /gene="flgL"
                     /locus_tag="IU325_01485"
     CDS             complement(30143..31096)
                     /gene="flgL"
                     /locus_tag="IU325_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-filament junction protein FlgL"
                     /protein_id="HAP5807707.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEADGKYEGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(31108..32751)
                     /gene="flgK"
                     /locus_tag="IU325_01490"
     CDS             complement(31108..32751)
                     /gene="flgK"
                     /locus_tag="IU325_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HAP5807708.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASAEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(32817..33758)
                     /gene="flgJ"
                     /locus_tag="IU325_01495"
     CDS             complement(32817..33758)
                     /gene="flgJ"
                     /locus_tag="IU325_01495"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HAP5807709.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEEPTAAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(33758..34855)
                     /gene="flgI"
                     /locus_tag="IU325_01500"
     CDS             complement(33758..34855)
                     /gene="flgI"
                     /locus_tag="IU325_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HAP5807710.1"
                     /translation="MIKFLSALILLLVTTAVQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(34867..35565)
                     /gene="flgH"
                     /locus_tag="IU325_01505"
     CDS             complement(34867..35565)
                     /gene="flgH"
                     /locus_tag="IU325_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HAP5807711.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKS
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(35618..36400)
                     /gene="flgG"
                     /locus_tag="IU325_01510"
     CDS             complement(35618..36400)
                     /gene="flgG"
                     /locus_tag="IU325_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HAP5807712.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(36537..37292)
                     /locus_tag="IU325_01515"
     CDS             complement(36537..37292)
                     /locus_tag="IU325_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HAP5807713.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(37312..39237)
                     /gene="flgD"
                     /locus_tag="IU325_01520"
                     /pseudo
     CDS             complement(37312..39237)
                     /gene="flgD"
                     /locus_tag="IU325_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706995.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
     gene            complement(39249..39653)
                     /gene="flgC"
                     /locus_tag="IU325_01525"
     CDS             complement(39249..39653)
                     /gene="flgC"
                     /locus_tag="IU325_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HAP5807714.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(39657..40073)
                     /gene="flgB"
                     /locus_tag="IU325_01530"
     CDS             complement(39657..40073)
                     /gene="flgB"
                     /locus_tag="IU325_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HAP5807715.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            40229..40888
                     /gene="flgA"
                     /locus_tag="IU325_01535"
     CDS             40229..40888
                     /gene="flgA"
                     /locus_tag="IU325_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HAP5807716.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLHTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            40964..41257
                     /gene="flgM"
                     /locus_tag="IU325_01540"
     CDS             40964..41257
                     /gene="flgM"
                     /locus_tag="IU325_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HAP5807717.1"
                     /translation="MSIDRTSPLKPVSTVQPRENTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            41262..41678
                     /gene="flgN"
                     /locus_tag="IU325_01545"
     CDS             41262..41678
                     /gene="flgN"
                     /locus_tag="IU325_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HAP5807718.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNAANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(41718..43253)
                     /gene="murJ"
                     /locus_tag="IU325_01550"
     CDS             complement(41718..43253)
                     /gene="murJ"
                     /locus_tag="IU325_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HAP5807719.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(43363..44286)
                     /locus_tag="IU325_01555"
     CDS             complement(43363..44286)
                     /locus_tag="IU325_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP5807720.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKKQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(44288..44935)
                     /locus_tag="IU325_01560"
     CDS             complement(44288..44935)
                     /locus_tag="IU325_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HAP5807721.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPVVTAMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(44946..45530)
                     /gene="rimJ"
                     /locus_tag="IU325_01565"
     CDS             complement(44946..45530)
                     /gene="rimJ"
                     /locus_tag="IU325_01565"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HAP5807722.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            45766..46974
                     /gene="mdtH"
                     /locus_tag="IU325_01570"
     CDS             45766..46974
                     /gene="mdtH"
                     /locus_tag="IU325_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HAP5807723.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            47038..47685
                     /gene="grxB"
                     /locus_tag="IU325_01575"
     CDS             47038..47685
                     /gene="grxB"
                     /locus_tag="IU325_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HAP5807724.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            47819..48379
                     /locus_tag="IU325_01580"
     CDS             47819..48379
                     /locus_tag="IU325_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP5807725.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            48485..49531
                     /gene="pyrC"
                     /locus_tag="IU325_01585"
     CDS             48485..49531
                     /gene="pyrC"
                     /locus_tag="IU325_01585"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP5807726.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGGMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRNGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            49605..49850
                     /gene="dinI"
                     /locus_tag="IU325_01590"
     CDS             49605..49850
                     /gene="dinI"
                     /locus_tag="IU325_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HAP5807727.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            50140..50394
                     /gene="bssS"
                     /locus_tag="IU325_01595"
     CDS             50140..50394
                     /gene="bssS"
                     /locus_tag="IU325_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HAP5807728.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            50509..51627
                     /gene="solA"
                     /locus_tag="IU325_01600"
     CDS             50509..51627
                     /gene="solA"
                     /locus_tag="IU325_01600"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HAP5807729.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEEDPIFVRSGVINLGPADSTFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            51675..51788
                     /locus_tag="IU325_01605"
     CDS             51675..51788
                     /locus_tag="IU325_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2770 domain-containing protein"
                     /protein_id="HAP5807730.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            52049..52615
                     /locus_tag="IU325_01610"
     CDS             52049..52615
                     /locus_tag="IU325_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HAP5807731.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLQSYSPMTRLGAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            52619..53194
                     /locus_tag="IU325_01615"
     CDS             52619..53194
                     /locus_tag="IU325_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HAP5807732.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PLATFTSTSVKKDGDDLDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(53236..54288)
                     /locus_tag="IU325_01620"
     CDS             complement(53236..54288)
                     /locus_tag="IU325_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP5807733.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALDVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            54513..55433
                     /gene="lpxL"
                     /locus_tag="IU325_01625"
     CDS             54513..55433
                     /gene="lpxL"
                     /locus_tag="IU325_01625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferase"
                     /protein_id="HAP5807734.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            55605..56831
                     /gene="mdtG"
                     /locus_tag="IU325_01630"
     CDS             55605..56831
                     /gene="mdtG"
                     /locus_tag="IU325_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HAP5807735.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            56914..57288
                     /locus_tag="IU325_01635"
     CDS             56914..57288
                     /locus_tag="IU325_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HAP5807736.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWGEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(57289..57516)
                     /locus_tag="IU325_01640"
     CDS             complement(57289..57516)
                     /locus_tag="IU325_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HAP5807737.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(57689..60232)
                     /gene="mdoH"
                     /locus_tag="IU325_01645"
     CDS             complement(57689..60232)
                     /gene="mdoH"
                     /locus_tag="IU325_01645"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HAP5807738.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDADHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKSWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(60225..61760)
                     /gene="mdoG"
                     /locus_tag="IU325_01650"
     CDS             complement(60225..61760)
                     /gene="mdoG"
                     /locus_tag="IU325_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HAP5807739.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYDAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            62154..63311
                     /gene="mdoC"
                     /locus_tag="IU325_01655"
     CDS             62154..63311
                     /gene="mdoC"
                     /locus_tag="IU325_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HAP5807740.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTFAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(63319..64800)
                     /locus_tag="IU325_01660"
     CDS             complement(63319..64800)
                     /locus_tag="IU325_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HAP5807741.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIVLKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(64742..65275)
                     /locus_tag="IU325_01665"
     CDS             complement(64742..65275)
                     /locus_tag="IU325_01665"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HAP5807742.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHLAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENTHLYERLLTQQGDE"
     gene            complement(65370..65681)
                     /locus_tag="IU325_01670"
     CDS             complement(65370..65681)
                     /locus_tag="IU325_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAP5807743.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSSAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGRTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(65802..66134)
                     /gene="csgC"
                     /locus_tag="IU325_01675"
     CDS             complement(65802..66134)
                     /gene="csgC"
                     /locus_tag="IU325_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HAP5807744.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(66192..66647)
                     /gene="csgA"
                     /locus_tag="IU325_01680"
     CDS             complement(66192..66647)
                     /gene="csgA"
                     /locus_tag="IU325_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HAP5807745.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKDSTMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(66688..67143)
                     /gene="csgB"
                     /locus_tag="IU325_01685"
     CDS             complement(66688..67143)
                     /gene="csgB"
                     /locus_tag="IU325_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HAP5807746.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT        16498 a        17901 c        16592 g        16553 t
ORIGIN      
        1 aaacgcattt ttctcgctgg ctaacgtttt ttacgttcgc cgctgccgtg gcgctggcgc
       61 taccggcaaa agccaacacc tggccactgc cgccagcggg cagtcgtctg gttggcgaaa
      121 acaaatttca tgtggtggaa aatgacggtg gttctctgga agccatcgcc aaaaaatata
      181 atgtcggctt tctcgctctg ttacaggcta accccggcgt tgatccttac gtgccgcgcg
      241 cgggtagcgt gttaacgatc ccgttgcaaa ccctacttcc agatgcgccg cgcgaaggca
      301 ttgtgatcaa tattgcggaa ctgcgtctct attactaccc gccgggtaaa aattcggtaa
      361 ccgtgtatcc catcggcatt ggtcagttgg gtggtgacac gctgacgcca acgatggtga
      421 ctaccgtttc ggacaagcgt gcaaacccaa cctggacgcc aacagcgaat attcgcgccc
      481 gttataaagc tcaggggatt gagctacctg cagtggttcc ggcaggaccg gataacccaa
      541 tgggccatca tgcgattcgt ctggcggcct atggcggtgt ttatttgctt cacggtacga
      601 acgccgattt cggcattggc atgcgggtaa gttctggctg tattcgtctg cgtgatgacg
      661 atatcaaaac actctttagc caggtcaccc ctggtaccaa agtgaatatc atcaacaccc
      721 cgattaaagt ctctgccgaa ccaaacggtg cgcgtctggt tgaagtacat caaccgctgt
      781 cagagaagat tgatgacgat ccgcaactgc tgccaattac actgaatagc gcaatgcaat
      841 catttaaaga tgcagcgcaa actgacgctg aagtgatgca gcatgtgatg gatgtccgtt
      901 ccgggatgcc ggtggatgtc cgccgtcatc aagtgagccc acaaactctg taaatcctgg
      961 ataaaaaaaa tccccgcgac acggcatgtc gcggggaaaa aatcagcagt agcaggcatt
     1021 aatgagggtt aatgcttatt tataaattac tgctgttcca tggagggtat ttggaccggt
     1081 tacagaagta atacggaatg attttgcgcc catctcatcc gctttttgcg ccagctgctc
     1141 ttccagcgat cccagatttg tccccgcgtt agcactgata gtaccgactt tttgttggcc
     1201 ttctggcgtt gactgaactt cgacagccgc aaagctggca aatgacattg agcttaaaat
     1261 cgccgcagcg atgagggttt ttacgttttt cataatagtg gccttatgca gatgaatgac
     1321 gtcgcaagca attacttaac gatcgataac taaatgatag atgtgatctg gatcacatac
     1381 aagatatttt ttataacaat cattaattaa tttaaaaatc tttaaatttc agttaaatag
     1441 aaagcaatta tttttatggg atctggactg gtgaacggag gtgattattt ttataataac
     1501 ctttcattca cagtcaccag gtacaacggc atcatggcaa ctgactcaac acaatgtgta
     1561 aaaaaaagcc gtggccgccc aaaagtgttc gacagggatg ccgcgcttga taaggccatg
     1621 aaattgttct ggcaacacgg ttatgaagcg acttctcttg cggacctcgt cgaagcgacc
     1681 ggagccaaag cgcccacgct atacgcggaa tttaccaaca aagaggggtt atttcgcgct
     1741 gtactcgacc gctatatcga tcattttgcc gctaagcatg aagcacagct gttttgtgaa
     1801 gagaaaagcg tggagactgc gctggctgac tattttgctg ccgtcgccaa ctgctttacc
     1861 agcaaagaca ccccggctgg ctgctttatg atcaacaact gcaccaccct ctccccggat
     1921 tccggagata tcggcaatac gctgaaatca cgccatgcga tgcaagagcg cactttgcag
     1981 cagtttttat gtcaacgaca agcgcgcggg gaaatcccga cccactgtga cgtgacgcat
     2041 ctggcagaat tccttaattg tattattcag gggatgtcga tcagcgcacg cgaaggtgca
     2101 tcgctggaaa aactgatgca gattgcccga acgactttgc gtttatggcc ggaactggtg
     2161 aaataaggcg caaaaaaagc ccctcaacct gagcgaatga ggggctaaat tgcagagtac
     2221 agccttgtta tactttgtta ttcaaaatca gttgcccgtt gccatctaac gggatctgcg
     2281 tacccggatc gcggtccatg cggattttgc cctgctgatc gccaatctta taggtcacat
     2341 cataaccgag cattttttct gacttgtcat acaccgtttt acaacgctgt tgcgtagtcg
     2401 tgtaagtatc gctttcctgg agagagccct ggatctggtt accggcatag ccacctccca
     2461 gcgcccccac aacagtggcg acatctttac cgcgaccacc gccaaactga tgccctatca
     2521 cgccgccagc aacagcgccg agcaccgacc cggtaatgcg attttcatcc tgcaccggtc
     2581 gacgatgggt cactgtgacg ttgcgacact cctgacgcgg tgttttaacc gtttccttga
     2641 ttggggttgc agaaacaacc tgagcgtatt gcgggccacg ttcaaacacg ttcagactgg
     2701 ccactgccgc tacgcccaac gcagctgcga caccaatccc gatacccgcc aacattgatt
     2761 tattcacggg aacacctcct tctttgctgt tatgccaatc atgcaagcga aggagaaaag
     2821 cagctatcag acagaagatg aaaagcgcgg gaatggctgg aaatacttag ggattcggag
     2881 gagtctgaag gaatgcctga tgcgacgctt aacgcgtctt atcaggccta ccgctcgtac
     2941 catttgtcca gggcggataa ggcgtttacg ccgcatccgc cagtacaagc cgattaatgc
     3001 aacttcaaac gtggacggat aacgcggtta atactcccca ccagcatcat taatccggtt
     3061 ttaaagtaac catgcagcgc aatctggtgc attcggtata gcgagatgta tacaaagcgc
     3121 gcaattcgtc cttcaatcat cattgagccg cgcgtcaggt tacccatcag gctaccgacg
     3181 gtggagaagt tcgacagcga taccagagaa ccgtgatctt tatactgata atttttcagc
     3241 ggcttaccgt tcatctgcgc cagaatgttg ttcattgcgc aggttgccat ctggtgtgcg
     3301 gcctgcgcac gcggcggaac aaagccccct tccggacgcg ggcatgacgc gcagtcgcca
     3361 atagcgtata tgtctggatc gcgagtggtt tgcagcgtcg gttccaccac cagctggttg
     3421 atacggttgg tttcaagacc accgatatct ttcaggaagt ctggtgcttt gatcccggct
     3481 gcccacacca tcagatcagc ctcaatatat tcaccatctt tagtatgcag gccgccttca
     3541 tcagcactgg tgaccatggt ttgcgtcagc acgcgaacgc caagtttcgt taactcatta
     3601 tgggccgcag cagagatacg tggcggtaat gcaggcaaaa tacgttcgcc cgcttctacc
     3661 agcgttacgt tcagggcttc gttggtcaga cctttataac cgtagctgtg cagttgcttg
     3721 accgcgttgt gcagttcagc ggagagctct acacccgtcg cgccgccgcc gacaatcgca
     3781 atgttcactt tgccattcgc gcccaggttg gcggagtatt tcaggaacag attcagcatc
     3841 tcctggtgga agcgacgcgc ctggtgcggg ttatcgagga aaatgcagtt ctctttgaca
     3901 cctggcgtat tgaaatcgtt agaggtgcta cccagcgcca ttaccagggt gtcataggcg
     3961 attttacgtt ccggaaccag caattcacct ttttcgtcgc gcagttctgc aatagtgatt
     4021 gttttcgctt cacgatcaat atcaattacg gaacccagct ggaactggaa accatgattg
     4081 cgcgcatggg ccagatagct caacgcatcg acgccttcat caagcgagcc agtcgccact
     4141 tcgtgcagca gcggtttcca caggtggctg tggttacgat cgaccagcgt gattttggct
     4201 tttttcttgc gtcccagctt atgccccagc tgtgttgcca tttccagccc accagcaccg
     4261 ccgccgacaa tcacaatttt tttcaatggc gtagtcaacg tgaccccctt aaatttatta
     4321 accaattgtt aattaatggt tattggcata gctcttaatt aacaacaggt tacagcaatg
     4381 aaaatgttct tcgctattga gaataacatg aatggtgcat tggtcatacc aaaattgatg
     4441 ttaatcaagt tttgttgctg aaaagatagg cgactttacc gggcgagatc aaaaaacaga
     4501 gtttgttgtt tattatttga gctaatttat gagagtggta tagcgtgagg ggtgtatgga
     4561 ggacgtttgg taggcctgat aaaatgcgca tcaggtaccg gcaaggttac ctgatgcgac
     4621 gagggcattt acccgagggt tttgaacgct ttaatgcgct gtaaatgcgg ggagatattc
     4681 ttgaatttgt gcgtctgctc ttcgtcccag acaatctcgt aataatgatg cagctcttca
     4741 gatgtccgct ggctgttaag cgcttcatca ttacgcgaca aaatcaccag gcaacgatcg
     4801 cgattctttt cgcggaagtt ggtcacacac ttagtggcaa tatcggcata ctcttccggg
     4861 cgatcaatct tgccttccat gttctcataa gggaacaaat tagggttgaa gatcacctgg
     4921 cggatatcgc aaagaaaacc aatccgttcc gcccagtatc cgcccagacc aacgccgcaa
     4981 atcagcggac gctcgtcgac gttcagttgc aacattttgt ccacttcttt gagcagatgc
     5041 tgcatatcat gtttcggatg ccgcgtactg tagcttatca agcgtacatc cgggtcaata
     5101 aactgcaatt gtaagacttt ctcgtggtta cccggactgt tagagtcaaa accgtgtaaa
     5161 tagataatca tcgcttcctc acataagcca gggttagtga cctgctttct cttcctgcca
     5221 gcgttcatgt aactgattca gacgggcgct gatcgctttc cagcgagccg agtccatcag
     5281 ttcctgtcgc gaaaatgaac ctttatgata caaacgtgta acacgttctg ccttgatcgg
     5341 tgacagatta tctaacacgc tgacggcccc tttacgatta ttgcagacca ggatcatatc
     5401 gcaacccgcg tccagtgatg cctgcccgcg ttcggcataa ctgcccataa tcgcggcacc
     5461 ttccatcgat aaatcgtcag agaaaatcac gccatcaaaa cccagttcct gacgcaaaac
     5521 ggttttcagc caatagggag aaccgctcgc cggacgcgga tcgacatcac tgtagatcac
     5581 atgcgcaggc ataatggcgt cgagtttatt ttcgcggatt aaggaactga agaccgacat
     5641 atctttagcg cgaatctccg cttgcggacg tgggtcacac ggtgtttctt tgtgtgagtc
     5701 tgccgttact gcaccgtgtc ctgggaagtg tttcccggta gttttcattc cggcttcatg
     5761 cataccatca ataaaccggc tggcaattgc cagggctttt tcgggatcgg catgataaga
     5821 acgctcgcca atcgccgcgc tgatatgccc gacgtccagc actggcgcaa agctgatatc
     5881 aatatccata gcaatcattt cgctggccat caaccaacct gcctcttgcg ccagttttcc
     5941 gccttcttcc attcccaaca gcgcagcaaa tgattgtgcc gctggcaagc gggtaaaacc
     6001 ttcacgaaaa cgctgcacgc gtccaccttc ctgatcaacc gccaccacca gatgattgcg
     6061 cgaagctgcg cggatctggc acaccagttc acgtaactgg gcaggatcat gatagttacg
     6121 cgtaaagaga atcagccctc ccaccagcgg atgcgccagt atttcacgct cttccgcgtc
     6181 cagttcgtaa ccttcgacat ccaacattac tggacccaca ctgctctcct tattgtttta
     6241 ttaatagctg ccgccaggtg tcatcggcca gcctgataaa ttgttgatcg ccggtttgtc
     6301 gccaacggta ctcaaaccac cctgctttga gcatcagcag ccagggaaac catcgcctga
     6361 cctgacgcca taattgcgcc ggataaatct tcgcgcgagt ggcatagtca ttgaccaatt
     6421 gccggtgctg gtcagtattt tccacccaca ccgccgccag ttccagcgcg atatcaccat
     6481 ctcctgcata ctcccagtcg atgagtttta accctgacgc gctatgcact aaatttccgg
     6541 cgtggacatc catatgcaat ggacttaagc gtaaaggccg tggttcccgc gctttgcgca
     6601 gacgttttaa ccttcgcagc caacccactg tccgccgcgc cggatcgctt tgctgccagt
     6661 acagttccag taacggcaac agcgttattc gccagccaaa acgcggttgt tgatgtagat
     6721 aatacagcaa gcctgccagt tcgttggtat ccggcaaata cgtttttacc tcgccgggca
     6781 gatagtcgac caccatccag tcacggagat ataaatgcgg cttcggtgca atgcatgcgg
     6841 gtagttgtga taaagcccga tactggcgca agaacgcgga ctgcggcgca tcaggatcgt
     6901 gcggctgacg cacaacaaaa cgctggccct gatgttcaat gagaaaactc ccgccactaa
     6961 gcccactatt aaacgtcgtg acgggatgaa attgcgggaa aaagcgcgac agcaattcgt
     7021 cgcgcgtgat gggattattg ctgcgaaacg gcacctttac ctgaccagat aatttcgccc
     7081 gtctgcacca gcatcagctg catttgtagg gtcggagcgt taacgttgcc agaggcactg
     7141 gagtatagca cgtaatgagc gccgacattg cgggcaatgc ctatggcttt actacgggtg
     7201 cctaaactgt cctgcggcga caaacctaac tgttgctttg ccatcgacag ctgctgggcg
     7261 gaaaccaggg taaatttacc gttattagcc agcgcatttc gcagcgtttc ggtcgcttct
     7321 gcggcattca gcgaaccgtt agtacggttg ttaacgctat caaccagcag gacgctaccc
     7381 gcagtcacgc cgtcagcccc aagcatctta ctgaccatcg gctgcattgc accattccag
     7441 tcataatggc gaatatgcgg cgcaggcggt gcagtttgat cttcgtgctc aattgggcct
     7501 ggctgctgcg ggatcgtcgg caccgagggc actgtgggga caggctgttg tggctcggct
     7561 ggttgttccg gcgctggttt cacttcttct accggtgcag gttcacgttg ccccacacac
     7621 ccggcgagaa acatcgccag cgcggtaatc aaggcgtagc gactcatttt tgtcatcaag
     7681 attcacccct tacaaatata gataaagtct gactttgtgc gcccccagaa aattggcgct
     7741 gccgtacagc gttaccgccg aatgtgcggg aatggtaacg ctgcgtggcc tttccagagg
     7801 atgcatctcc agccctctgg cgtcatacca gtaaaaacga taatgaacgg taacgggttc
     7861 ttgcctttcg ttataaagcg ttgaggatgc tgaaggttga atatcagacg tcgaaaggac
     7921 gggcttttct gcactgatgc ccgcagccaa taaagatgac tccatcacca gcgattgctc
     7981 atcattcacc ggaatttccg gatgtgaacg acagcccacc agcactaaca acgccagaga
     8041 caccagccca aagcatcctt ttctcatcgt tacagacctt tatgcgccag cattggtccc
     8101 agcggacggc cacccaacaa gtgcatatgg atgtggtaaa cctcttgtcc gccgtggcgg
     8161 ttggtgttca tgatcagacg atagccatct tcggcaatac cttcttgctc agcaattttt
     8221 gccgctacgg tgatcatgcg tcccagcgcc tgctcatgct cagcagacac gtcgttcaca
     8281 gtcggaatga ggatattcgg aatgatcaga atatgcgttg gcgcttgcgg cgaaatatcg
     8341 cgaaacgccg ttaccagatc atcctggtag acgatatcgg aggggatctc acgacgaata
     8401 attttgctga atatagtttc ttctgccacg acattttcct ttttcaaata gccatgcgtt
     8461 gcgccatgct acgccgcgcc acactgcgag tatagagtat ggtcgagtta aacctgctct
     8521 ttcaacctta actcacgatt tcttaagcaa aaaatcgttt tgctgccgta tatatcgcca
     8581 ttattcccat ttctgcctgc gaatgcgctg aaaagacatt ctctgtttgg tttacacgcc
     8641 aaacatgaat gcgtatattt ctcatttgca tttacataca aaattattcg cacactaaat
     8701 ataaccgctc atcaccctcc tccggcatga gcctgacgac aacataaacc aagagatttc
     8761 aaatgctttc aacacaattt aacagggata atcaatatca agccatcacc aaaccgtcac
     8821 tacttgccgg ttgcattgta ctggcactat taccttctgc cgcttttgct gcaccagcca
     8881 ctgaagaaac ggtgattgtt gagggttcag ccacagctcc agatgatggc gaaaatgatt
     8941 acagcgtaac gtctacctct gcaggtacca aaatgcagat gactcaacgt gatattcctc
     9001 agtcggtcac tattgttagc cagcagcgga tggaagatca gcagttacaa acgctgggcg
     9061 aagtgatgga aaacacgctg gggatcagca aaagtcaggc ggattccgat cgtgctcttt
     9121 attattcccg cggattccag atcgataact atatggttga tggtatcccc acctattttg
     9181 aatcgcgctg gaatctgggc gacgcacttt ctgatatggc actgtttgaa cgcgtagaag
     9241 tagtgcgtgg cgcgacagga ctcatgaccg ggacgggtaa tccatctgcg gcaattaata
     9301 tggttcgaaa acacgcgacc agtcgtgaat ttaaaggcga tgtctcggcg gaatacggta
     9361 gctggaacaa agaacggtat gtggcggatt tacaaagccc actcaccgaa gatggtaaaa
     9421 tccgcgcgcg aattgtcggc ggctaccaga ataacgactc atggctggac cgctacaaca
     9481 gtgaaaagac cttcttctcc ggcattgtcg atgctgattt aggcgatctt acgacgcttt
     9541 cagccggtta cgaatatcag cgcattgatg ttaatagccc aacctggggc ggtttaccgc
     9601 gctggaatac tgatggcagc agcaacagtt acgatcgcgc acgcagtacc gcacctgact
     9661 gggcgtataa cgataaagag atcaacaagg tctttatgac cctcaagcag cggtttgctg
     9721 atacctggca agcgacactg aatgccaccc actccgaagt cgaatttgac agtaaaatga
     9781 tgtatgtcga tgcctatgta aataaagcgg atggtatgct ggttgggcca tacagtaatt
     9841 atggacctgg ctttgattat gtcggcggca ccggttggaa cagcggcaaa cgtaaagttg
     9901 atgcgctgga tttgtttgct gacggtagtt atgaattgtt tggtcgtcag cacaatctaa
     9961 tgtttggtgg cagttacagc aaacaaaaca atcgttactt cagttcatgg gcaaacatct
    10021 tcccggatga aattggcagt ttctacaact ttaatggcaa tttcccacaa accgactggt
    10081 caccacagag cctggcgcag gacgatacca cacatatgaa atcgttatat gctgccactc
    10141 gtgtcaccct tgccgatccg ctgcatctga tcctcggcgc tcgttatacc aactggcggg
    10201 ttgatacgct gacttacagc atggagaaaa accacaccac gccttacgct ggtctggtgt
    10261 ttgacatcaa tgacaactgg tcgacctacg ccagctatac ctctattttc cagccgcaaa
    10321 atgatcgtga cagttcaggc aaatatctgg ctccaatcac cggtaacaac tacgagctgg
    10381 gtctgaaatc ggactggatg aatagccgtc tgaccaccac gttagccatc ttccgtattg
    10441 agcaggataa tgtcgctcag tccaccggta cacctatccc cggcagcaac ggcgaaaccg
    10501 cctataaagc ggtggatggg acagtcagta aaggtgtgga atttgaactc aacggcgcaa
    10561 ttaccgacaa ctggcagctg acgtttggcg caacgcgcta tattgcagag gataacgaag
    10621 gaaacgccgt taatcctaat ctgccacgca ccacggttaa aatgttcacc agctatcggt
    10681 tgcctgtcat gccagagttg acggtcggcg gtggtgttaa ctggcaaaat cgcgtgtata
    10741 ccgacaccgt gacgccgtat ggcaccttcc gcgccgagca aggtagctac gcgctggtgg
    10801 atctcttcac ccgctaccag gtgacgaaaa acttctcgtt acaggggaac gtcaataacc
    10861 tgttcgacaa aacctacgat accaacgtgg aaggttctat cgtctacggc gcaccgcgta
    10921 atttcagcat taccggcacg tatcaattct gaataacacc tgtaaaaaag gcagccatcc
    10981 ggctgcctta gtctccccaa cgtcttacgg attagtggtt acggatgtac tcatccatct
    11041 cggttttcag gttatcggat ttagtaccga aaatcgcctg aacaccagaa ccagcaacca
    11101 ctacgcccgc tgcgcccagt ttcttcaggc cagcctgatc cactttagac acatcagcaa
    11161 cgctgacgcg cagacgggta atacatgcgt cgaggttagt aatattttct ttaccaccaa
    11221 atgcagcaac cagagccggt gccatttcgc tggtacctgt cgcttttgca tcttcagtcg
    11281 catcttcacg acccggcgtt ttcagatcca gtgctttaat cagcacgcgg aagatggtgt
    11341 agtaaacaat tgcataaccg ataccgacga tcgggaacag ccacagtttg ctgctgttac
    11401 cagacagaac aatgaagtcg atcagaccgt gcgagaacga cgtaccgtca cgcatcccca
    11461 gaagaataca gattgggaat gccaggcctg ccagaatcgc gtggatgatg tacaggatcg
    11521 gcgcaacgaa catgaaggag aactcgatcg gctcggtgat accggtcagg aacgaggtca
    11581 gcgccgcgga gatcataata ccgcccactt tcgcgcggtt ttctggttta gcagagtgcc
    11641 agatagcaat tgcggcagct ggcagaccgt acattttgaa caggaagcca ccagacagtt
    11701 tacccgcagt cgggtcaccc gccatataac gcggaatgtc gccgtggaaa acctgacctg
    11761 ctgcgttggt gtattcacca atttgcatct ggaaaggtac gttccagatg tggtgcagac
    11821 caaacggtac caggcaacgt tcgatgaaac cgtaaatgcc aaacgcaact accgggttct
    11881 ggtaagcagc ccactgagag aaggtctgga ttgcagaacc aatcggcggc caaatgaagg
    11941 acagcacaac gccagtaaag atggcagcca ggccagaaat gatcggcaca aaacgtttac
    12001 cggcaaagaa gccaagatac tcaggcagct taatacggta gaaacggtta aacatgtacg
    12061 ctgcgatcgc accggagata atccccccga gtacgccagt atccgccagg tgtttagagg
    12121 cgatttcttc agcaggtaaa tgcagtacca gtggcgcaac cacggccatg gttttaacca
    12181 tgatgccata ggcaacaact gcggccagcg cggatacgcc atcgttattg gtaaagccga
    12241 gggcgacacc aatcgcaaaa atcagtggca tgtttgcaaa gacggaaccg cctgcttctg
    12301 ccataacatg cgatacaacg gcgggcagcc agctgaaatt cgcggaaccg acgcccagca
    12361 gaatacctgc gataggcagt acggataccg gcagcatcag cgatttaccg accttttgca
    12421 ggttagcaaa tgcattctta aacataattg agagtgctcc tgagtatggg tgctttttta
    12481 cgttctcacg cgtggcaagg ggggagagcc tcgccgtgta cagggcatct aagcgccctt
    12541 tatttattac acagagtaaa ataattcagt gccgatttgt ttgacggcta tcacgtttca
    12601 acttcagatc attggagaag ttgcacattt tgacaaaaca tatgtcacaa tatttcaaac
    12661 gtggttattc accgcccaat tttcggcggt gaactttact cgttttagcc attaattacg
    12721 agctttaaaa aaactcattc aacggatgga ttgaaggcgg gaagcgtcga tgtgaaacag
    12781 acgggcgaag ttatcggtgg ttacctgcgc cagttcttca acggcaacac ctttcaacac
    12841 agccatgtat tctgcaacgt cacgaaccat cgcaggttga ttctcttttc ctcgatgcgg
    12901 caccggcgca aggtaaggtg agtcagtttc taccagtaac cgatccaggg ggacataacg
    12961 cgcagcatcg cgcagttgct ccgcattacg gaaggtcaca atgccggaaa aggagatgta
    13021 aaatccgaga tccagtaatt tacccgccgt ttctctgtcc tctgtaaaac agtgtagtac
    13081 gccaccgcaa tccgtcactt tttcttcgcg cagaattgcc agcgtatcgg cgcgggcgtc
    13141 acgggtatgg acgataaccg gcttgttcag ttcacgacca atctggatat gatggatgaa
    13201 ggactcttgc tgccgtactt tagtttccgg cgtgtaataa taatccaacc cggtttcacc
    13261 cagcgcaaca acgccctctt ctgccgccag acggcgtaaa tcttctacat cgtagggatc
    13321 attctggttg agtggatgca cgccacaaga aaatacgacg ttgtcacgtt cgcctaccag
    13381 atcccgcatg tgtaaataac ccggtaatgt tgtggcgact gccagacaaa atttcacatc
    13441 gcgtgcggcg gctttcgcca gaacgtcatc cacgtcctta tgcaaagatt cataatccag
    13501 accatcgaga tggcagtgtg agtcgactaa aaacatgatg tctctcttaa agatgaggaa
    13561 ccggtagcac aacgcccggt tgcaggtaat gctcaatacg cagtaaaaga tcggtgatga
    13621 gaagctcgcg gttgatgcct gtgacggaca ttaactgttc acgaatgtgg caaacatccc
    13681 ccagtatagc ctgcaggcgc gagggagaaa gatggtttgc cagttcgacg accagccccg
    13741 gcacatcaac attggtcacc tgcgcagcgc cataatggcg ttttagcgca tccatcagca
    13801 acgttgccag ccagtgtaaa cgcgccggag cttgttcatg attaagggcc gctagcagcg
    13861 aataccagtc gcccgattgc acgctatatg ccagcgcctg acacaatgtt tcacgagcct
    13921 gccagttatc tccctgaaac aacgccagtg ccgcgccagg cgaaccggcg cttaagcgca
    13981 atgcggcaag tagtgcatcc tgtgacattg tcacttcgcg tgaaagccag gtcacggcgt
    14041 actgttccgg cggcggcgca aggtaatgta accgacaacg actacgtaat gttgccagta
    14101 agcgttcagg ctcgcgggta gccaggaaaa accaagtttc tgctggtggc tcttcaagcg
    14161 ttttcagcaa tgcgttagcc gcggcgtcag tcagtaaggc agcatcggtt acccagacaa
    14221 ctttcgcacc acctaagcgt gcgtgctcat ttaacttttc ggtgacttca cgtaccgcat
    14281 caatgcccag cgcatttttc cctttttcgg gagccagggt gtaataatcg ggatgcgtgc
    14341 cagcctgcat caactgacat ccacgacagt gaccgcaact tttgtggccc tgcggttgtt
    14401 ggcagagtaa ataacggctc agggcgtaga ttaaagcatc atcgcccatg cccggtaacg
    14461 cctgaatgag cagcgcatgg tgacctcttc cggcctgata gctggctacc agtttttcga
    14521 aatcaggtcg taaccatgga taccatctca tgcgtccaac tccttcaccc agttggtcac
    14581 ggtagtgcgg attgcatcca tcacggcctc cagcggctgg gtggcatcaa tggtatgaat
    14641 gcttttatct tgtgctgcca gttccagata gcgggcgcgg gtgcgattaa agaaatcgaa
    14701 agattcttgc tcaatacgat ccagctcgcc gcgcgcacgc gcgcgtttta agccaacctc
    14761 cggggtaaca tcgagataga gcgttaagtc ggggcgaaaa tccccgagaa cagcatcacg
    14821 cagtgttgcc agcatatgtt ggtcaatacc acgtcctcca ccctgatagg cttgagtgga
    14881 gagatcgtgg cgatcgccaa tcacccaggt gccgttagcc agcgcaggtt tgatgaccgt
    14941 ttctaccagt tgaacgcgcg cggcataaaa catcagaact tcggctttat cggtaatgac
    15001 ttcatcgcct accgatttga tatccagcac caggcttctt aacttttcgg caagttgcgt
    15061 accgccaggt tcccgagtga aaaccatgtc gcggataccc agttgctcga gcgtctcaac
    15121 caccacgtta cgcgcggtag ttttgcctgc gccttccagc ccctcaatga cgatatactt
    15181 actgcgcatt tttttcctta agcactttca gataatcctg cacagacttg ttatgactgg
    15241 caagattggt attaaacgtg tgaccacctt taccatcggc cacaaaatag agatacggcg
    15301 tttttgccgg atgcgcagca gccttcagcg aatccgcccc cggcgtcgct atcgcacctg
    15361 gcggcaaacc ggtaatggta taggtgttat acgctgtcgg cgtttccagg tctgcacgag
    15421 aaagtttgcc attataacgc tctcccatcc cgtaaatcac ggtcgggtcg gtctgcaggc
    15481 gcataccaat gcgtaaacgg ttgataaata ccgaggcaac ctgatcgcgt tcgctggaaa
    15541 cggcggtttc tttttcgata attgatgcca tcgtcaccag ctggttttta tctttatagg
    15601 gcagaccgtc cgcacgccct tcccaggcgc tatcgaccgc tttcaccatt ttcttgtgcg
    15661 ctcgcttgag taacgcgaca tcggtggtat tggcggtata catccaggtg tctggccaga
    15721 accaaccttc aatccactcc gggttttcca gttcaagtgc ctgcgctacg gtggcgtact
    15781 tatcgtcgct cagcgtgtgc ttgatatacg gggcctcacg caattgcttg aggtaatcgc
    15841 tcagacgcat cccttctacc agtcgcagag ggaactgtgc ttctttaccg ctttccagca
    15901 atttcagcat ctcgcgcacg gtcatctgcg gtgtaaagcg gtaagtcccg gctttaaagt
    15961 gagaaagatc cggttcgata cgcagcagcc attggaaaac ccgtgggcga ttgatgatct
    16021 tatcggcata aagctgttca ccgagcgcca gacgtccggt ccctggcttc agggtaaata
    16081 tcgtctcttc tttgataagc aatttgctgt cggcaagatg gcgaaccttc cagacgccca
    16141 caccagcggc gatacccagt accaccagca ataacaagat aatcaataac acttttttca
    16201 tgactaattc gggcgctcac aaagtggggc taaatattca tataacgttg ctgacgaaaa
    16261 ggagacatcg ccacaggcac gtacgggtat cactggcatt aacgcattac aaataaccat
    16321 ctcatctgcc tgcaacgcct cctccagcga ggcttgcact tcgacaagct gataagagga
    16381 ttgtgccagt aaacggatac agaattgtcg cataatgccg ttaacacctg cctgatccag
    16441 tcgcggcgta taaactacgt tgcccttccg ccagaacaaa ttagccgcac agcattccgt
    16501 aacccaccct tcgctgtcaa ggaccagcgc ctcatcagcg tttgtctgct caagatgaga
    16561 gcggatcaat acttgctcaa gccgattaag atgtttaata cctgcaagat gaggattgcg
    16621 ccccagccgc accgggctta gcgccaacgt catcccctcg ttacgcaaac ggtcgtaatg
    16681 tgcgggataa accgtaacgg agagaatccg cgttgccggt ccgctgttca atgtgctgta
    16741 tcctcgcccg ccactaccgc gactgatcac cactttcagc acaccatttt gctgttctgc
    16801 tgccagcgtt ttcatctcta gttcaagctg aggccagaag tcacaggaaa tcatcaaccg
    16861 ctgacaagca tcctgtagtc gctggatatg cgccgataac aaactgactt tgccgtcgat
    16921 aactctggcg gtggtaaaac aaccatcacc aaactgcgtt gcccgatcgc ttactgccag
    16981 cgattcctgc ttatgaccgt taattaagaa cataccggct ccttatcaga gaattgttag
    17041 tgtggcagca tgttcactac ggaacaagtc ggaataaaag ctaagaaaaa aggcccgcaa
    17101 gcggaccttt tataagggtg gaaaatgaca atttagatct ttttaaagat caaagaacca
    17161 ttagtgccac cgaagccgaa ggagttacac agcgtgtatt ccattccgct aacctgacgc
    17221 gcttcgtgcg gtacgaaatc cagatcgcaa ccttcatccg ggttatccag gttgatggtt
    17281 ggcggaacag cctgatcgcg cagcgccagg atggagtaga tagattctac tgcacccgcc
    17341 gcacctaaca ggtgaccggt catagatttc gtggagctta ccaacacacg gcttgcagct
    17401 tcaccgaaga tggttttcac cgcctgcgct tcagctttat cgccagccgg cgtagaagta
    17461 ccgtgcgcgt taacgtagcc aatctgactc gcttcaatgc ctgcatcacg cagagcattt
    17521 gccatcgcca gagctgcgcc tgcgccattt tctggcggtg acgtcatatg ataagcatcg
    17581 ctgctcatac caaagccgac gagttcagcg taaattttcg caccgcgttt tttcgcgtgt
    17641 tcgtactctt caagtactag cataccggca ccatcgccca gtacgaaacc atcacgctct
    17701 ttatcccacg ggcggctcgc cgcttgcggg ttatcattgc gggtagataa tgcacgtgcc
    17761 gcgccaaaac caccaacgcc cagcggcgta ctggctttct ctgcgccacc tgcaaccatc
    17821 acgtcagcat cgccatacgc gataatacgc gcagcatggc caatgttgtg cacgccggaa
    17881 gtacaggcgg tcgcgataga gatgctcggg ccacgcaggc catacatgat agtcagatga
    17941 cctgccacca tgttcacaat cgttgacgga acgaagaatg ggctgatctt acgtggacca
    18001 ccgttcatca gagatgtgtg gttttcttcg atcagtccga ggccgccaat cccggagcca
    18061 attgcggcac caatgcgggt tgcgttctct tccgttattt caaggccaga atcctgcatg
    18121 gcctgaacgc cagcgacaat tccatattga atgaaggcat ccatcttgcg ctgttctttg
    18181 cgcgagataa tgtcctcaca gttaaaatcc tttactaagc cagcaaattt cgttgcatag
    18241 gcgctagtat cgaaatggtc gattaggctg atgccactct gaccggcaag cagagctttc
    18301 caggtagact ctacggtatt gccgacagga gacaacatgc ccagtccggt cacaactaca
    18361 cgacgcttag acacgtttgt cctccaggga gggaaaaaat gattctagtg ggacaaaaag
    18421 ataaaactca ggcggtcgaa cgaccgcctg gagatgttca cttacgcctg gtggccgttg
    18481 atgtaatcaa tggcagcctg aacggtggtg attttctcag cttcttcgtc cggaatctca
    18541 gtatcaaact cttcttccag agccattacc agctcaacgg tgtcaagaga atccgcgccc
    18601 aggtcttcaa cgaaagaagc attgttggta acttcttcct gcttaacgcc cagctgttcg
    18661 ccgataattt tcttaacgcg ttcttcgata gtgctcatac tcttaaattt cctatcaaaa
    18721 ctcgctttcg cgatggtttt cgtagtgtat aaaatgttga aaaatttgca actaaatccc
    18781 ggcaggtctt accacgattt tacgttattt tgaggcctta ccccccaata acgcaaatca
    18841 ttttgcacta attattactc ataaccacgc agactgcgcg caacatgagc attttgtgca
    18901 aatcgcggtc agaccatgta catcccgccg ttcacatgca aagtttcacc cgtgatgtaa
    18961 gctgcttcgt cggatgccag gaatgcaacc gcgttggcga tttcctgtgc gccgccgagg
    19021 cgacccgcag gaacctgcgc caggataccc gcacgctggt catcgctcag cgcacgtgtc
    19081 atgtccgttt caataaagcc cggagcaaca acgtttacag taataccgcg tgacgcaact
    19141 tcgcgcgcca gtgatttact gaagccgatc aagcccgctt tcgccgcagc gtagttggcc
    19201 tgaccgccat ttcccatggt accaaccaca gaaccgatag tgataatgcg accatgacgc
    19261 tttttcatca tagcgcgcat taccgctttt gacagacgga aaacagatga aaggttagtt
    19321 tcgataatat cgttccactc ttcatctttc attcgcatta acaggttatc acgagtgata
    19381 ccggcattat tgaccaggat atccacttca ccaaattctg cgcgaatttt ttccagaaca
    19441 gattcgatag atgccgggtc ggtcacattc aacatcagac ctttgccgtt ggcacctaaa
    19501 taatcactga tcgcctgagc gccattttca ctggtcgcag tgccaataac tttcgcgcca
    19561 cgggctgcga gcgtttcagc aattgcgcgg ccaattccgc ggcttgcacc ggttaccagt
    19621 gcgatttttc cttcaaaatt catgattttc ctcttttaaa gctcgagcgc cgctgccatc
    19681 gctgaaggtt cgttcagcgc cgaggcggtc agggtgtcga caatgcgttt cgtcaggcca
    19741 gtaagcactt tgcccgggcc gacttcatag agatgttcta cgccttgcgc tgccatgtat
    19801 tcaacagact tcgtccactg aaccgggtta tacaactgac gtaccagtgc gtcacggatg
    19861 gcatcaccat tggtttcgca tttcacatca acgttattca caacaggaac tgttggtgcg
    19921 ttaaaggtga ttttcgctaa ttctactgcc agtttgtcag ctgctggttt catcagcgca
    19981 cagtgagacg gtacgctcac tggtaacggc agagcgcgtt ttgcgcccgc agctttacag
    20041 gcagcgccag cacgctcaac tgcttcttta tgaccagcaa taaccacctg tcccggagag
    20101 ttaaagttta ccggagaaac gacctgacct tctgcagctt cttcacacgc tttcgcaata
    20161 gacgcatcat ccagaccgat gattgccgcc atagcgcccg ttccttccgg tacggcttct
    20221 tgcataaact taccgcgcat ctcaaccaga cgcaccgcat cagcgaaatc aatcacgcca
    20281 gcgcatacca gtgcggagta ttcccccagg ctgtgaccgg ccatcattgc cggtgcttta
    20341 ccaccctgct gctgccatac gcgatacagc gcaacagatg cagtcaacag cgctggctgg
    20401 gtttgccagg ttttattcag ttcttcagct ggcccctgct gggtcagcgc ccacaggtcg
    20461 tagcccagcg ccgcagaagc ttcagcaaac gtttcttcga caattggata gctcgccgcc
    20521 atatcagcca gcattccaac ggtttgagaa ccctgtccag ggaacacaaa tgcaaattgc
    20581 gtcatgtttt aatccttatc ctagaaacga accagcgcgg agccccaggt gaatccaccg
    20641 ccaaaggctt caagcagaac caactgcccc ggcttaatgc gcccgtcgcg tacagcttca
    20701 tccagcgcgc acgggacaga ggccgcagag gtattaccgt ggcgatccag cgtcaccacg
    20761 acattgtcca tcgacatgcc gagttttttc gctgttgcac tgataatacg caggttagcc
    20821 tgatgcggaa ccagccagtc cagttgagaa cggtcaagat tattcgccgt cagcgtctca
    20881 tcaacgatgt gcgccagttc tgttaccgca accttgaaga cttcgttgcc cgccatcgtc
    20941 agatgaattg aattctctgg attcacacgg tcagcattag gcagcgtcag caactcacca
    21001 tagctaccgt cggcatgcag atgggtggag atgattcccg gctcttcaga ggcagccagc
    21061 accgcagcgc ccgcgccatc gccaaaaata ataatagtcc cacgatctgt tggatcgcag
    21121 gtgcgcgcca gtacatcgga accgacgacc agagcatact tcaccgcccc agatttcacg
    21181 tattgatcgg ctacgcttaa tgcataggtg aaacctgcgc aggctgctgc aacgtcaaat
    21241 gccgggcaac ctttaatgcc cagcatgctt tgaatctgac aagctgcgct cgggaaagcg
    21301 tgcgtagcag aagtcgttgc cacaacgatc aggccaatct ggtctttctc aatgcccgcc
    21361 atctcaattg cgcgtgtcgc cgcttcaaag cccatggttg aaacggtttc gtttggcgcg
    21421 gcaatgtggc gttcgcggat accggtacga gtgacaatcc actcgtcaga ggtgtccacc
    21481 attttttcca aatcggcgtt tgtccgcact tgttcgggca gatagctgcc agtaccaata
    21541 atcttcgtat acatgtacgc tcagtcactt ttcggttata taccgtcact tgcaaactgc
    21601 gagttcgctg gcagcgtcct gctaccgcag agttccgctt ttgccaccgt ccagcagctc
    21661 aaaaccagct gggtatacag attccaggcg agcggcaatt cgctgaggaa cttgtcgctg
    21721 caccgcctgc actgcctgtt caatcgcgac cgcaaaagct cgctgattgg ctgcaccatg
    21781 actttttatc accgtgccgc gcaatcctaa cagacaggcg ccgttatact ggtcggggtt
    21841 gaggtgactg aatcgcctcg tcaggctctt ttgtagccaa cgctttaata acagtagcca
    21901 ccacgaccgt tttttccctt caccctgaga tttcagcaga gaaaggaaca tcctgacaac
    21961 accttccatc gtctttaatg tgacatttcc tgtaaagccg tcacaaacca gcacatctgt
    22021 cttgccagtt aacaactcat tggcttcaag atagccgata taattgatag aagggattgt
    22081 tttaagcacc gctgaggcat cccgaatact gtcgagaccc tttacttctt cttcaccaat
    22141 attgagcaac gccacgcgag gattgggaat ttccaccacc tcttcagcca gaactgagcc
    22201 cataatggca aattgcacca acattgtact gtcacaatcg acgttggccc ctaaatcgag
    22261 gaccaccgtt ttgccctttt gctgatgcgg taataccgtc accagcgccg gacgctcaat
    22321 cccctccagg ggcttgagta ataattttgc cagccccatc agcgccccgg tattaccggc
    22381 actgacacag gcttgcgctc gaccttcttt caccagctcc agggccacgc gcattgaact
    22441 cccacgactg gcgcggatag cttgcgaagg ccgggcatca ctggcgataa ctgactgcgc
    22501 aggaataatc tgcagacgcg aacgttgttc aaagtcagct ttagcaagta atggcgtgat
    22561 ggcgtcggga ttgccgacta aaagaagagt gagttgcgaa ttagaattca gtgcctgcaa
    22621 tgctgcaggc actgtcacgg aagggccaaa atcccctccc atgacatcta acgccagggt
    22681 tagacgtgtc aaggtatcgt cgccgcccgg tttggtcttt ccccagttgc ctggggaaaa
    22741 tcctcactaa gcttcatcac gcagacgcgt gattacttag cgatgacctt gcggccgcgg
    22801 tagtaaccgt cggcagtgat gtggtgacgc aggtgttttt caccagaagt tttgtctaca
    22861 gacaggctgg tgactgcggt cagcgcgtca tgggaacgac gcatgccacg tttggaacgg
    22921 gttggtttat tctgttgtac ggccatggac cttactcctc aattacggtt tatccccgat
    22981 ccaacgggga gcaccaatta cttacgcttt aagctggcta atacggcaaa tgggtttggc
    23041 ttttgcgctt cttcaggcag ttcaccaaag accatgtccg cttcggacac ttcacagtgt
    23101 tcagaatcat gcaccggaac taccggcaag gcgaggatga tttcatcttc aaccattgca
    23161 agcagatcga tttcaccgaa ttcgttaacc tcaatcggtt catacgcttc cggcagtgct
    23221 tcagcctgtt cgtctgaacg cacaggacta aaacaatacg ttgtgtagac ctgatgagta
    23281 aacggcttcc cgcaacgctg acactcgagc gttaccgtca ccttcgcatc gccgtttaac
    23341 actgcgagac gttggttatc gatagcgaac gacatggagc attccacatc actgtccaca
    23401 ctgactacgg attcggcgac gcgctcaacc tgatcagggg tatagatacc ctggtaatca
    23461 aggcgttttt gagccgtacg aaccggatcg agagtcaggg gtaattttac cttttgcata
    23521 gggcgcgcat attaactttg taacgtcata gagtcaaaga aaaaggcagc ctgtggttgc
    23581 cttttgccaa taattcgcac acattgcggg ttactgcttt attttcgttc agtgctgata
    23641 acgaacatca acctgcaaaa cgaagcgcat agtttaaaat ggctatcatc aatacgctat
    23701 atctgatgga aaaaaatatg cctaaactta ttttagcctc cacttcgccc tggcgtcgcg
    23761 ctcttctgga aaaactgcaa atctcttttg aatgtgcagc accagaggtc gacgaaaccc
    23821 cacgcagcga cgaatcaccg cgacagttgg tgcttcgact ggcacaagaa aaagcacaat
    23881 ctctggcgtc acgttatccg gatcatttaa ttattggttc agaccaggta tgtgttcttg
    23941 atggcgaaat caccggtaaa ccgttaacgg aagaaaatgc ccgtctgcaa ttacgcaaag
    24001 ccagcggcaa tatcgtcacc ttctatactg gactggcgtt gtttaattcg gcgaatgggc
    24061 atctacaaac agaagtggag ccttttgacg tccatttccg tcacctgagc gaggcggaga
    24121 ttgataatta cgtgcgtaaa gagcatcccc tgcactgcgc gggtagcttt aagagtgaag
    24181 gatttggcat tactctgttt gagcgtttag aggggcgtga tcctaacacg ctggttggtt
    24241 taccgctaat cgcgctgtgt cagatgttac gtcgggaagg gaaaaacccg ctgatgggat
    24301 agttgtttgc gccggatgcg gttacgtcgc atccggcaat cggtgctggc ggcctgatgc
    24361 ggcgtaaacg ccttatcagg ccaaccgccc attatcatgc ttatatgctc gtattagcgc
    24421 gcgttacgca gcttttgcaa acaacgcttc aaaccatcat ccatcggcgc ttcgatacgc
    24481 atcacctcac cggtccccgg atgggtaaac ttcaacgctg cggcgtgcag gaacagacga
    24541 tttaatcccg tgccggcttc agtgagctgt ctgtcaaatt cacggtcacc gtagcgatcg
    24601 tcaaaggcaa tcggatgacc cgcatactgt gtatgcacac ggatctgatg agtacgccct
    24661 gttaccgggc tacaacgcac cagggtggca aatgcatagc gttcttccac tttaaagcgt
    24721 gtttccgacg gtttgccttc ctgactgaca cgcacgatac gttcgccgct ttgcagaata
    24781 tttttcagta acggcgcttg aacgctcttc acatgcgact gccactgacc gcgcaccagc
    24841 gccaggtaat ctttttgcat ccctttttca cgtaattgct catgcagaga acgcaacgcc
    24901 gagcgttttt tcgctaccag caacacacct gaggtgtccc ggtcaagacg atgaaccagt
    24961 tcaaggaacc gcgcttccgg gcgcaacgcc cgcaaacctt cgataacgcc gaagcttaaa
    25021 ccactgccgc catgtaccgc cgtaccggaa ggtttattca gcaccaggat atggtcatct
    25081 tcatataaga tgacgtccgc cagcgctgcc accttttgca gatgtggcga aaccgcctct
    25141 tcttcccgct cagcaacgcg aaccggtggg atacgtactt catcacccgc ttcgagttta
    25201 tattcaggct taatacgttt tttgttcacc cgcacttcgc ctttacgcaa aatacggtaa
    25261 atcatacttt ttggtacgcc tttcaattgg gtacgcaaaa agttatcgat acgttgcccc
    25321 gcttcgtcag cggtgatagc aacaattttt acggatggag tctctgtttt catggtgggc
    25381 gattctaatt agccaacagg attcgcgcca ctcatttttc tatgcttata tttagttttg
    25441 caccttatta cttcactacg tgatcacttt attgatggtt attaaaccaa tcaccagcaa
    25501 gaagtgaaaa aactgtgagt aagcgggtga taaatggtaa aagtcatctt gctataacaa
    25561 ggcttgcagt ggaataatga ggccgtttcc gtgtccatcc ttgttaaaac aagaaatttt
    25621 acggaataac ccattttgcc cgaccgatca tccacgcagc aatggcgtaa gacgtattga
    25681 tctttcaggc agttagcggg ctgcgggttg cagtccttac cggtagatgg aaatatttct
    25741 ggagagtaat acccagtctg tttctttgat aattgcgctg tttttccgca tgaaaaacgg
    25801 gcaaccgaca ctctgcgcct ctttgagctg acgataaccg tgaggttggc gacgcgacta
    25861 gacacgaggc catcggttca cacccggaaa ggcgttactt tgcccgcagc ttagtcgtca
    25921 atgtaagaat aatgagtaag ttacgatgaa aagaatgtta atcaacgcaa ctcagcagga
    25981 agagttgcgc gttgcccttg tagatgggca gcgtctgtat gacctggata tcgaaagtcc
    26041 agggcacgag cagaaaaagg caaacatcta caaaggtaaa atcacccgca ttgaaccgag
    26101 tctggaagct gcttttgttg attacggcgc tgaacgtcac ggtttcctcc cactaaaaga
    26161 aattgcccgc gaatatttcc ctgctaacta cagtgctcat ggtcgtccca acattaaaga
    26221 tgtgttgcgt gaaggtcagg aagtcattgt tcagatcgat aaagaagagc gcggcaacaa
    26281 aggcgcggca ttaaccacct ttatcagtct ggcgggtagc tatctggttc tgatgccgaa
    26341 caacccgcgc gcgggtggca tttctcgtcg tatcgaaggc gacgaccgta ccgaattaaa
    26401 agaagcactg gcaagccttg aactgccgga aggcatgggg cttatcgtgc gcaccgctgg
    26461 cgtcggcaaa tctgctgagg cgctgcaatg ggatttaagc ttccgtctga aacactggga
    26521 agccatcaaa aaagccgctg aaagccgccc agctccgttc ctgattcatc aggagagcaa
    26581 cgtaatcgtt cgcgcattcc gcgattactt acgtcaggac atcggcgaaa tccttatcga
    26641 taacccgaaa gtgctcgaac tggcacgcca gcatatcgct gcattaggtc gcccggattt
    26701 cagcagcaaa atcaaactgt acaccggcga gatcccgctg ttcagccact accagatcga
    26761 gtcacagatt gagtccgcct tccagcgtga agttcgtctg ccatccggtg gttccattgt
    26821 tatcgacagc accgaagcgt taaccgccat cgacatcaac tccgcacgcg cgacccgcgg
    26881 cggcgatatc gaagaaaccg cgtttaacac caacctcgaa gctgccgatg agattgctcg
    26941 tcagctgcgc ctgcgtgacc tcggcggcct gattgttatc gacttcatcg acatgacgcc
    27001 agtacgccac cagcgtgcgg tagaaaaccg tctgcgtgaa gcggtgcgtc aggaccgggc
    27061 gcgtattcaa atcagccata tttctcgctt tggcctgctg gaaatgtccc gtcagcgtct
    27121 gagcccatca ctgggtgaat ccagccatca cgtctgcccg cgctgctccg gtaccggtac
    27181 cgtgcgtgac aacgaatcgt tgtcgctctc tattctgcgt ctgatcgaag aagaagcgct
    27241 gaaagagaac actcaggaag ttcacgccat tgttcctgtg ccaatcgcgt cttacctgct
    27301 gaatgaaaaa cgttctgcag tgaatgcgat tgaaactcgt caggacggcg tgcgctgtgt
    27361 gattgtgcca aacgatcaga tggaaacccc gcactaccat gtgctgcgcg tgcgtaaagg
    27421 ggaagaaact ccaaccttaa gctacatgct gccgaagctg catgaagaag cgatggcgct
    27481 gccgtctgaa gaagagttcg ctgaacgtaa gcgtcctgaa caacctgcgc tggcaacctt
    27541 tgccatgccg gatgtgccgc cagctccaac cccagctgaa cctgccgcgc ctgttgtagc
    27601 cccagcacct aaagctgcaa cggcaacacc agcatctcct gcacaaccag ggttgttgag
    27661 ccgcttcttc ggcgcactga aagcgctgtt cagcggtggt gaagaaacca aaccggccga
    27721 gcaaccagca ccgaaagcag aagcgaaacc ggaacgtcaa caggatcgtc gcaagcctcg
    27781 tcagaacaac cgccgtgacc gtaatgagcg ccgcgatacc cgtagtgaac gtacagaagg
    27841 cagcgataat cgcgaagaaa accgtcgtaa tcgtcgccag gcacagcagc agactgccga
    27901 gacgcgtgag agccgtcagc aggctgaggt aacggaaaaa gcacgtacca ccgacgagca
    27961 gcaagcgccg cgtcgtgaac gtagccgccg ccgtaacgat gataaacgtc aggcgcaaca
    28021 agaagcaaag gcgctgaatg ttgaagagca atctgttcag gaaaccgaac aggaagaacg
    28081 tgtacgtccg gttcagccgc gtcgtaaaca gcgtcagctc aatcagaaag tgcgttacga
    28141 gcaaagcgta gccgaagaag cggtagtcgc accagtggtt gaagaaactg tcgctgccga
    28201 accaattgtt caggaagcgc cagctccacg cacagaactg gtgaaagtcc cgctgccagt
    28261 cgtagcgcaa actgcaccag aacagcaaga agagaacaat gctgataacc gtgacaacgg
    28321 tggcatgccg cgtcgttctc gccgctcgcc tcgtcacctg cgcgtgagtg gtcagcgtcg
    28381 tcgtcgctat cgtgacgagc gttatccaac ccaatcgcca atgccgttga ccgtagcgtg
    28441 cgcatctccg gaactggcct ctggcaaagt ctggatccgc tatccaattg tacgtccgca
    28501 agatgtacag gtcgaagagc agcgcgaaca ggaagaagta caagtgcagc cgatggtgac
    28561 tgaggtccct gtcgccgccg ctgtcgaacc tgctgttagc gcaccggttg ttgaagaagt
    28621 ggctgaagtt gtagaagccc ccgttcaggt tgccgaaccg caacctgaag tggttgaaac
    28681 gacgcatcct gaagtgattg ccgccgcggt aactgaacac ccgcaggtga ttaccgagtc
    28741 tgatgttgcc gtagcccagg aagttgcaga acacgcagaa ccggtggttg aaccgcagga
    28801 agagacggca gatattgaag aagttgccga aactgctgag gttgtggttg ctgaacctga
    28861 agttgttgct caacctgccg cgccagtcgt cgccgaagtc gcagcagaag ttgaaacggt
    28921 agccgcggtt aaacctgagg tcaccgttga gcataaccac gctaccgcgc caatgacgcg
    28981 cgctccggca ccggaatatg ttccggaggc accgcgtcac agtgactggc agcgccctac
    29041 ttttgccttc gaaggtaaag gtgctgcagg tggtcatacg gcaacgcatc atgcctctgc
    29101 cgctcctgcg cgtccgcaac ctgttgagta ataattagct caagtaatca agccctggta
    29161 actgccaggg cttttttatt tcagggtagc atctttgttg taatgacatt agcgttacat
    29221 gcgctgtata attatcatcc atgcaaagcc gaaggatata tttatgccag cagccaataa
    29281 cattccaacc aggcgcgaat ctttaaattt gcgcataaaa cccgcagaac gcgatcttat
    29341 tgaccgggcc gctaaagccc gaggaaaaaa tcgcaccgaa tttgtccttg aggccgccag
    29401 agccgcagct gaagaggcgc tcattgagca gagaattttc aggatcgatt ctgatgatta
    29461 ccagctattt ttgaatcgtc tggatcagcc ccccgcgccc aatgctgcat tacgcaaaac
    29521 catgcaaaca cccgcgccgt gggagcaaaa aaaatgattc aggcccccga gccactcatg
    29581 gcaaggcatc aattcacctc tttttgctcg ggagtagaaa cgatggataa ctggctaaag
    29641 cagagagccc taaaaaatca gcttgccggc gcatcacgta catttgttag ctgtgatacg
    29701 tatagcaacg ttttggcata ttattcgctt gcttccagcg ccgttgaaac gtatgtcgcg
    29761 acaggacgat ttcgccgaaa tatgccagaa ccgatccctg ttgtagtact tggtcgtctg
    29821 gcaatagata aatcattaca gggtcagggc atcggtcgtg cgatggtcag agatgccggt
    29881 ctacgagtac tacaggcggc tgaagtcatt ggtattcgcg gaatgttggt ccatgcgtta
    29941 tcagaccagg cccgtgagtt ttatctgcga gtggggtttg aaccatcgcc tgtcgattct
    30001 atgatactga tggcaacatt ggctgatttg caggaatgtc ttaaatagtt cgttatgtct
    30061 catgcaaaaa aatgcccgcc ccggcgatcc cagagcaggc agagaaaaca tacccagttt
    30121 catgatatgt tttaaagcga gcttatttgt tgagctggaa cagcgacaat ccctgcatat
    30181 cggtaaatgc tttatacgat gcctgcaatg ccgtttgctg catgatgtaa gatgaaatag
    30241 ttgcattcca gtccacatca accagatcgc tcatctgctg cgtttgccct aaagcgcgat
    30301 cgctacctaa tgaatccagc gactccagtt cgttcagctg cgtgcctaat tccgcgcgaa
    30361 cagtcagcac attgttcagc gagtttttca gtccgcggtt ggttttatcc agtgccgcag
    30421 cggcggtttc tttatccgct tcgctatccg cgaccggcgt ttttagggct gcgatggcgc
    30481 tatccagcat ggcaaaaaga ttggtttcag aagcgctacc gtctggttcc gctaccgcgt
    30541 tgctggtaat actgtcgaaa attttgtcac ccgtgtgccc tatcaccatc gaacgcgaag
    30601 catcgacctg ttgtttaata ctttctgctc cgccttcgta tttgccatcc gcctcactaa
    30661 acggcgcagt ttctgtttta taaccggcaa aaatgtagcg cccgttaccg tcagtggtgt
    30721 tcgccagatt cagcaactgg tcacgaagcc cctgaatatc cgtagccagc gaggcacggt
    30781 catcgtcact caaggtgcca ttgctggcgt agacaatttt ttcctgagca ttctggatag
    30841 cggtggtgac ctggctaagt acactctctt ccagtgacac tttttgagtg gcgaaagtac
    30901 gcgccagcgt gtactggctg ttttgcgcct gtgcctggga gagaacaaca gcttgtgatg
    30961 cagcaatggg atcatctgaa gggttaacga ctcgcttacc cgtcgacatc tgttcgccgt
    31021 acttcatcca ttctgcctga gaattggtga tgccgcgcat gttttgctgg tacatcatct
    31081 gggtactgaa acgcatttta tctcccctta gcgaatgtta atcagcgcat caaaaatcgc
    31141 gtttgccgtc tgcagaacct gcgcattcgc gaggtaatac tgctgaaaac gttgcagatt
    31201 tccgtactcc tcatcgagat tgacaccgga aatcgactgc tgctgattgg aaagctgcgt
    31261 caccacatta ccttgagtgg tgctactggt tttcaacgtc gcggttttat taccgatatc
    31321 actcactaac gaggcataag cgtcgttaaa ggatttcgca ccgcccaccg ttttactgtt
    31381 gctttgcaga tcgagcaggg cctgaccgtt gcggttgtcg ctatcacccg catcttctgc
    31441 gctcgccatg gcgattttcg cttcgtcggt gattaataca tccatgttga cgatggcgtc
    31501 acttaccggt ttcagcgtga agctgtcgtt aacggcaggc gttcctgtaa acgtcaactc
    31561 cagaccatca aatgccactt taccgttggc atccggcgtc accgtaaaag tggtattgct
    31621 ggcaaggcgg gtgacctgcc actgattatt atcgaacgag attttgtaat ctgtcgccag
    31681 taccgcggag gcatcagtta ccgttgcacc gatcgcaacg tcacctttgt ttttcgtgtt
    31741 ttgcagaacc gcgggcttac cgatagcaaa gaaatcttca ccggcatcgc cgttagcatc
    31801 aaatccggct ttgtgttggg tgttgaaagc ctcggcaaat gccagcgcca gttgtccaag
    31861 cgtattacgc gtctggtcaa gatcctgaga acggaatgtc agaatgccgc ccagcgaccc
    31921 ggtattcagt aatttctccg ggatctcaat attgcctgcc gtcccatcaa cataagcgac
    31981 agtcgtacga gaagggtcgg cgctggaagg aactgccgca agttgccgcg ccgtacttcc
    32041 ctgaaccagt gagtagccat tggccatcgt gatgttataa gtgccaccat cctgaacgct
    32101 gacttctacg ccaacaatct ggtttaattc gctcaccagt tgatcgcgtt gatccagcag
    32161 attgttaggt gacgcccctg cccccacgcc tgtcaggcgg gagatctgat cgttcaggct
    32221 ggcgatttgt ttagcgtaat tgttgatctg atcaacgctg gcacctatcg cgatattgac
    32281 ctgtttgtcc tggtcgcgca gatattgatc ggtggtttta aactgattca ccaatccttc
    32341 tgatttccca atcagcgcct ggcgcgctgc cggatcttcc gcgttactca ccagcgtttg
    32401 caggctggtg aagaaatcct gcatctgtgt tgccagcgaa gaggtactgg tggagagcat
    32461 attgtcgatt ttcgacatct gctcatagcg ggcggtcagg ccgctacttt gcgtctgcgc
    32521 agcacgtaac tggttggtaa taaacgcatc atactcacgc tgcacaccag aaacgtagac
    32581 gccattgcca acccagccgc cagcgcccaa cgtgctattg gcctgcgcca taatagtggt
    32641 ttggcgggta tatccggcaa cgttatagct ggagatatta ttacttgccg tatttaacgc
    32701 cgcctgggcc gcgttcagtc cgctcatggc gttattgatc aagctggaca tggaggttcc
    32761 ttataagcct tcaattacag agtattatcg gcagcgcccc gccggacttg agttattcag
    32821 aacagattat caatgttcat gctgtaggtt ttgctcacct tgtcgcttat cgatttcatc
    32881 tgctgaatca tgttggtgag tttgcgggca tagtgaggat cggtggcata gcccgcgtcc
    32941 tgtagggcct gcgccccctg ctccgcactc gcggcggtcg tcacggcggc gtagcgcggg
    33001 ttacgcgtta acagcccaac gtaatccgac aatgcttcca gatacgagct gtagacccga
    33061 aacttcgctt ttactttctt cgcttcgcca ttttcatatt cagtcgtggt gatttcagtg
    33121 actggccctt tccagttgcc agaggctttg acgccaaaca ggttatagct cggctcgccg
    33181 ttttcacggc ggatttgccg ttgtccccag ccagattcca gcgccgcctg agcgaggatc
    33241 aaatgatgtg gcacaccgct ttgctggctt gccagttggg cgggcaacga gagttgcgcg
    33301 aggaatgctt tactgtcacc cggcagcgaa tcatcgtagt tacgtggtac ggccttttgc
    33361 accagctgcg aaagcgtctg attttgataa cgcaccacgg tttcgagcgg gaatttcatc
    33421 ggtgctgctg ccgtgggctc ctctggcaat ggttgttctg gcgtcatctg tttaaccatc
    33481 atctctgcca gccccagacc tttgcccgcc gtcatctgtt gggcaatctg ctggtcatac
    33541 atactggtat acaggcgagt gtgctcgctg ctgaacaggc catcttttgg taaagcgtcg
    33601 cgcatgcttt tcaacatcat ctgcacgaac atcccttcca cctggcgggc caccggacgg
    33661 atatttgccg ccggatcttc gcccgctttc gccttaagtt cgttgagcga ttgtgcatcc
    33721 caggccgcac ttgccagtag tttgctgtcg ctgatcatca gatgatttcc agttttgccc
    33781 gcagacatcc cgcactttgc attgattgca gtatggacat cagatccatc ggcgtagcgc
    33841 ccagcgcatt gagcgcacgc accacgttat tgaggctggc gctagaacgt acgctttgca
    33901 gcgaaccgcc gctctggcgt aaatcgatct gcgtttgtgg agtaaccaca gtctgcccac
    33961 caccaaacgg tgtatctggc tggctgacat tggcctgacg attaactgtt actgagagat
    34021 tcccctgcgc taccgcgcag ctgtcgaggg tcacttcgcg attcatcacc accgaaccag
    34081 tgcgcgagtt aatcactact ttagcgtcct gcggggtaac attaacctgc atattctgga
    34141 tatcggcaag gaaacggacc tgggaactgt tgccactcgg tacgcgcacc tgaatagtcc
    34201 gcgcatctaa cgcggtggcg ctgccatatc cacgcacgcg gttgatggtg tcagcgattt
    34261 gctgcgccat actgaaatct tcgtcgttaa gttgcaaatt aagggtattc ccgacgccaa
    34321 actggctggg caattcacgt tcaataaccg caccattggt gatccgtcca ccgttcagtt
    34381 ggttcacctg aacactgcta ccgccagcgg aggcccctgc gccgccaacc agaatattgc
    34441 cctgcgccag cgcatacacc tgactgtcaa cgcccttaag cggtgtcatc aataacgtac
    34501 cgccacgcag acttttggca tttcccatgg aagaaaccac cacgtcaatg gtttgcccct
    34561 gacgtccaaa cggtggaagt gacgccgtca ccattaccgc agcgacgttt tttagctgca
    34621 tattggtgcc cgtcggaacg gtaattccca gttgtgagag catgttatta agcgtttgtg
    34681 tggtaaacgg cgtctgagtt gtctggtcac ctgtaccatc aagccccacc accaggccat
    34741 agccaatcag tgagttttgc cttaccccct gaacactggt gagatcgcga atacgctcag
    34801 cctgaaccgc cgtcgtaacc agtagaagaa ttaatgcaga gagaaattta atcaccacag
    34861 cctcacttac attggcgaca gattaaggaa gaaacgttgc aaccagccca tattttgcgc
    34921 ttcgttaatg tagccattgc ctacgtattc aatgcgcgca tccgccacct gagtagacgg
    34981 tacggtattg ctgccgctga tagtgcgtgg attaactaca ccagagaagc gaataaattc
    35041 ggtaccctga ttaatggcga tctgtttttc acccaccaca tgcaggttgc cgttgaccag
    35101 tacctggtca accgtcaccg tcaacgtgcc gctaaaggta ttgctggcat tggccccgcc
    35161 ttttccgttg aacgtgttac caccggaggc ttcgacatcg gcacgagcgt taccaaacag
    35221 cccctgcaaa tagcgcggca cagtatcaaa gccaaaatta gatttaccgt cacggctggc
    35281 attcgcagag gagcttttgc tggcgctgac gttctcctgc aacacgatgg tcagcgtatc
    35341 gccaatattg cgtggtcgac gatcttcaaa cagcggttga tagccatagt taatcggctg
    35401 agcagactgg aaaatagaac cgttggcgac gggcgtcgga ccgggaaccg gttgtgcact
    35461 ggtcgccccc tgtaccagcg gcgtggaggg tatccaggcg cagccggtta gtgaaagcac
    35521 caacaagctg gaaatggcat aagtatgcgc agcgtttttt tgcatggcta tcatcttcaa
    35581 aaatcagtaa accggtgaat ctgccaccgg ttatgcctta gagttgcgtc agtttttgca
    35641 gcatctgatc ggtggtggat accgctttac tgttgatttc gtaagcgcgt tgcacctgaa
    35701 tcatattgac cagttcttcc gccacgttga cgttagacgt ttcaacatac ccttgataca
    35761 gcagtcccgc gccgttcagg cccggcgtgc tttcgttcgg tgcaccggag gattgcgttt
    35821 cggtgtagag gttttcgcca atactctcca gtccggtatc attcataaag gtggtgagat
    35881 tgagctgccc aacctgaacc ggagctgcct ggccttgttg ggttacgctg accacgccat
    35941 cacgaccgat ggtgatactt aacgcattcg ccggaatggt gatcgctggc tgcacctgaa
    36001 aaccaccagc cgtcaccagc tgcccgttct gatccacctg gaaagagccg tcacgggtat
    36061 aggctgatga gccatctggc aacatcacct ggaaaaagcc ctgcccttta atcgcgacat
    36121 ctttgctgtt attggtttgc gacaggttgc cctggctgtg taagcgttca gtggcgaccg
    36181 ggcgtacccc cgtaccgatt tgtaatccgg atggtaaggt ggtttgttcg gaagactgtg
    36241 cccccggctg gcgaatggtt tgataaagca gatcttcaaa caccgcgcgc tgacgcttaa
    36301 aaccgttggt actaacgttt gccaggttgt tggcaatgac gtccatattg gtttgctggg
    36361 catccaggcc cgttttggcg atccataatg aactgatcat aaaatgtcct gtagtggatt
    36421 agtgacaagc agagcagaag cacgcgacgg tgcgaatttc ccctcaccct aacctctccc
    36481 aaaatgggcg agagaatagt ccgcgcgctt ttattgcaat gtcatcaatc gtttaattaa
    36541 ctcatcgaca acagttggtt ggcacggcct gcgttatcat cgacgctgct gatcaccttc
    36601 atctgcattt caaaacgccg cgcgctggca atcatgtcgc tcattgccgc aacggcattg
    36661 acgttactgc cttccagaac tcccgacatc acacgcaagg ttggatctgc ctgcagtacc
    36721 ggcccacgcg tggcctggct ttccgcgctt aaacgaaaaa tgccgtcgtc accgcgctgc
    36781 acttcgctgc ccgtggcttt caccagtttc agacgcccca ctggcgcaac cgtatttgcc
    36841 ggatcgcccg gattcagcgc cgagattgtg ccatcggcag caatagtgat ttccgcccct
    36901 tccggcacag caattggccc cgcctcgcct atcaccggat gcccctgaat tgtcagttgc
    36961 ccggtgggat caacctgaat gctgccatta cgcgtatacc cttcgctgcc gtcagcggtc
    37021 tgcacggcca gccagccatc ctgctgcaac gcaacgtcca gcgggcgcga ggtgtaatcc
    37081 atcttgccgg gcgtcatgtc tgcacccggc gttgacgccg ttaccagcgt gcgcgtgggc
    37141 agtgaaagcc cttccaccgg cactgcgcgc aaggcgttta actgcgcgcg aaaaccgggc
    37201 gtagaggcat tggccagatt actggcggtt accgcctgtt gattcaacgt ctggctggct
    37261 gctcccatcg cggtataaat tgcgtgatcc attgagctat cccgtcagcg attagcgtaa
    37321 gttaaccagc gtgttgagga tctggtcctg ggttttgatg gtctgggcgt tagactgata
    37381 gttacgctgg gcaacgatca tattgaccag ttctttactg agatcgacgt tggacgcttc
    37441 cagcgcaccg ttggtcaggg tgccaaagtt gcccgtcccg gctgtcccca acagcgccac
    37501 gccagaagat tgcgtcgcag accagacgtt gtcgccttcg gatgccagac cttcgttgtt
    37561 ggcaaagttc gccagtacaa tctgccccag cagttgggtt tgttcgttgg aatagttgcc
    37621 gacaaccgta ccgtcatcat tgatttgata actcaccaga tcgcccggtt tgtagccgtt
    37681 ctgggtggtt gccacaatgt tgttagcacc ggtattttgc tgcatggagt tgaggaaact
    37741 caggctaaac gtggcgggat ctgcgccgtt aattgcgcca gtggaaatat tcgctgttgg
    37801 atctgaggtc agaacgccat tggcattaaa caccaatgtt gtcgctgtct tcgcaatgct
    37861 gtttggatca ctactatcct gggtgtagac ctgccagtta ttatccccgg tcttcacaaa
    37921 gtagacgctc atgtcatgag cattaccctg actatcgaaa acagtcaccg aacctttttt
    37981 gttatagcta tccgcattgc tggcatcaaa tgcgttaaca gagggaagcg gatcactgga
    38041 attcaggttg atctgcatcg acgccgtggt ggtagttttc gctgccatca gggtattcgg
    38101 aatcgaaata ttggtcggat tcgccccttg ctgaatagtc ggcggcgtac cggttgccgg
    38161 gtaacccgtc agctgtaaac cttgcatatt caccaggtta cggttttcat ccagcttaaa
    38221 ttgtccgtta cggctgtaga acaccgaacc gttgctgtcc accagacgga aaaaaccgtt
    38281 ctggctgata gcaacgtcca gacctcgccc ggtgttggtg gtcgtgccat cggtaaagtc
    38341 ctgagtgata ccggcaactt ttacccccag tcccactttc gaaccggcaa acatatcggc
    38401 aaaagaggcc gtgcctgatt taaagccgta ggtggcggag ttggcgatat tgttgccaat
    38461 aacatcgagg ttggtggcgg cagcgtttaa tccgctaacc gcttgagaaa aggccatgac
    38521 tgactcctga aatgtgaagg cttaaattat ctgccgtact tcgtcgaggg tggtggtgcc
    38581 gtaagtgccg agatccagcg tattaccgtt gttgccgcgg atcacaccct gcaccagagc
    38641 aaactgcagc ggctgggcaa ccagttgtgt accaccgtta ctggcgctaa tcgctacatt
    38701 gtaagaaccg ttcggcgcag tgctgccgtc agtcaacgta ccgtcccagg tgaaactgtg
    38761 gactccggca gtcagttcac cgatatcaat ggtacggaca accgcaccgt ttttatcggt
    38821 gatggtggcc gtaactttgt ctgccgcctg ttgcagctca acaccaaacg gcgtggtcgt
    38881 ggtcacagcc ccttcttcac tgcctgttcc cgcaagaaca gtggtgccgg ggatcatcac
    38941 gccgtgaccg atcaggttac tggcctgtaa cgactggctg ttatcaatct gtccggaaat
    39001 agatccgagc gtggtattga gtttttcaat cccgctgacc gtgctgattt gtgccagttg
    39061 cgacgtcagc tcgttgtttt ccattggatt ggtcgggtcc tggtttttca gctgcgccac
    39121 caacaaagtc agaaaactgc tttgtaaatc tgcggcgttg ctgcccgtga gcgaactact
    39181 actggtggtg ctgacgccgg tatttgtcgg atcggtggtg gttaccgcaa tggacatagc
    39241 tttctccttt attgaccgag cgtaagggtt ttcagcatca tgcttttcac cgtgttgagc
    39301 acttcaacat tggcctgata gctgcgtgac gccgacatgg tgttaaccat ctccccgacg
    39361 acatcaacgt ttggcatttt cacgtagccc tttgcatccg ccagcggatt acccggttca
    39421 taaaccagtt tgtccggggc ctgactttct ataacatcgg caacttttac gccgcctgtc
    39481 gcagcacctg gcgcagcgtt aacctgaaat accacctgtt ttgcccgata tggctgtcca
    39541 tcgggaccgg tcacgctatc agcattcgcc agattactgg ccgccacgtt caggcgctgg
    39601 gactgagcag ttaacgccga cccggcgata tcaaaaatat tcagcagtgc catccgttaa
    39661 tttccgctct gtaaaacgtt catcatgcct ttgatttgcc cgctcaacgc gctaaggctc
    39721 atctggtatt gcaggctgtt atcggcaaac tgggtgcgtt cgcgatccat atcgacggta
    39781 ttaccgtcaa gcgaaggctg gtccggaata cggtattgca gttctgcggt aggaggtgtc
    39841 agcgcctgcg ccggaatgtg ttgcgttgag gtcatcgtca gtgcaaccac gcttgttgca
    39901 tcccgtccac gttgcatgac ttttttaagt tcactggcaa aatcgatatc gcgcgcctga
    39961 taaccagggg tatcggcatt ggcgatgttt gctgccagca cttcctgacg ctgggcgcgc
    40021 agattgagcg cctcttgttg aaaacgtaag gcggcgtcga gcttatcgag catatctcct
    40081 ccgcaggtat caaaattctg ccatcagctt aaatgccttt acgcgcgcgt tatcggcgga
    40141 ataaacgcaa aatgggtcgc tatttatgcc gttgatggtc attgcggaca ggtacaattc
    40201 acgttgtaga tatggctggg gggcgaaaat gctggcaata aaacgtagcg tggcgatcat
    40261 cgcgatactg ttcagtccgt taagtgcggc gagcaatctc acatcgcaat tgcacacctt
    40321 ttttagcgcc cagctcgcgg gggtaagtga tgaggttcgt gtttctattc gtacagcgcc
    40381 caatctacta ccgccgtgcg agcagccatt gctttcgatg agcaataatt cccgcctgtg
    40441 gggcaatgtg aatgtgttgg cacgctgcgg taacgacaaa cgatatttac aggttaatgt
    40501 gcaggccaca ggaaattatg tggttgccgc aatgcccatt gtgcggggag gaaagctgga
    40561 agctggcaat gtcaaactga aacgcggacg gctggacacc ttgccaccgc gtacggtgct
    40621 ggatatcaat caacttgttg atgccgttag cctgcgcgat ctatcacccg atcaacctat
    40681 ccagttaacc cagtttcgcc aggcatggcg ggtaaaagcg ggacaacgcg tcaacgtgat
    40741 cgccagtggt gatgggttta gcgccaacgc agaaggtcag gcgctgaaca atgcagccgt
    40801 cgcacagaat gcgcgggtgc gcatggtatc gggacaggtg gtcagcggcg ttgttgatgc
    40861 agatgggaat attcttataa acctgtaagc tgttaaagat tacccgtccc ttgccgataa
    40921 ataagcaaca catgataaaa gcgccctcaa tgaggaataa accatgagta ttgatcgcac
    40981 ttcgcctctg aagcccgtaa gcaccgttca accgcgcgaa aacactgacg cgccggtaac
    41041 gaacacccgg gcggcaaaaa caaccgcctc caccagcacc agtgtgacgt taagcgacgc
    41101 gcaagcaaaa ctgatgcaac ccggcagcag tgatatcaat cttgaacgtg tcgaagcgtt
    41161 aaaactggcg attcgtaacg gtgaactaaa aatggacacc ggcaaaattg ccgatgcgct
    41221 gatcaacgaa gcgcagcaag acttgcagag taactgatcg tatgacgcgt cttgcagaga
    41281 tcctcgacca gatgtccgct gtgcttaacg atctcaaaac ggtaatggat caagagcagc
    41341 aacatctctc tatggggcag atcaacggca gccagttgca atggattaca gaacaaaaaa
    41401 gctctctgct ggcgacgctg gattacctcg aacagttacg caggaaagaa cccaatgcag
    41461 caaatagcgt tgatattagt caacgctggc aggaaattac tgtgaaaacg cagcaactac
    41521 gccaaatgaa tcaacataac ggctggttac tggaaggaca gattgagcgc aatcaacagg
    41581 cgctggaaat gttgaaaccg catcaggaac cgacgctata tggagcgaac ggtcagacct
    41641 caacaaccca tcgcggcagt aaaaagattt cgatctgaag attatctccg gcctgcactg
    41701 caggccggaa tacattgtta caccgtccgg cgggcaaact ctttaacttt gaagcccagt
    41761 accgccagtg cagcaaagta cgcagcaatc cccgccagca cgaccgccat taaacgcagt
    41821 aagcgccagg gcatggtacc caatgaccac tccggcatga tatgtaacat acctaaaagc
    41881 acgcccgaca tcaccagtac cgccaccacc agacgcaaca gaaacgccat ccagccaggt
    41941 tgcggggtaa agattttctg cttacgcaac tgccagtaaa gcagcgaagc attcagacac
    42001 gccgccagac caatagaaag tgacagcccg gcatgtttca acggaccgat aaacgcaagg
    42061 ttcatcaatt gcgttaaaat cagcgtaacg atggcaattt tcactggcgt tttaatgtcc
    42121 tggcgggaat aaaagccagg agccaacact ttcactacaa tcaggccaat caaacccacc
    42181 gagtaggcaa ttaacgcccg ctgggtcatc agcgcatcaa aggcggtaaa tttaccgtac
    42241 tggaacagcg aaacggttaa cggacctgaa agaatgccca acgcaaccgc actcggcagc
    42301 gccaacagga aacaaagacg cagcccccag tccatcaaac ggttgtattc atcatgattg
    42361 ccactggcaa aacttttcga cagcgacggc agcaaaatcg tgccaagcgc cacgcccagt
    42421 acaccggacg gaaactccat taagcggtcg gcgtaataca tccaagacac cgaaccggaa
    42481 gcaagaaacg aggcaaaaat ggtgttgatg attaaggaga tctgactcac agagacgcca
    42541 aggatcgccg gtcccatctg tttcaccacg cgcattgctc cggcatcgtg gaagttaatg
    42601 cgcggcagga ccagcatgcc gatcttcttc aggtgcggta gctgatacac cagctgcagg
    42661 acgccgccga ccgtaacagc ccacgccagc gccagcaccg gtgggttaaa gtacggtgcg
    42721 gcaaacagcg cgaaaccaat catgctgatg ttaagcagtg ttggagcaaa cgccggaatc
    42781 gagaagcggt tccacgtatt cagaatcgct cccaccagcg acgccaggga gatcagcaag
    42841 atatagggaa aggtaatctt tagtagctgg ctggtcaggg caaatttgtc agctgtgtca
    42901 gcgaagcctg gcgcggtcac catgatcacc caaggtgcag cgagcatgcc tgcgaccgtc
    42961 acaaccgcca gcgcgagtgt caacagacca gaaacatacg agacaaagac ccgcgtggcg
    43021 tcttcaccct gcttgctttt gtattccgcc agaatcggta caaatgcctg ggaaaatgcc
    43081 ccttcggcaa agatacggcg taacaagtta ggaagtttaa aagcgacgaa aaaggcgtcg
    43141 gttgccatcc ctgcgccaaa gattctggcg acaattgcgt cacgtgcgaa gccaagcaca
    43201 cgcgaaaaca tggtcatcga gctgacggcg gccagcgatt ttaataaatt catcggtgtt
    43261 ctaatccaga cccacgggca aaacgcctgc aaggcaggcg ctgaaagagg cgaatagtct
    43321 acctggatta tggtgaattg ctaccgccag atgttacagg gtttattcac tcatcgcatc
    43381 gcgccagatc ttgtcaacga tacgttgcgc cagcactgcc tgttcgccgg cggtttgcgg
    43441 aactgtctga ttttgcacgc attcaatgaa gtgccgcgca cagccgacaa atccacgttg
    43501 ctcaagcgta ctctgccaac caggaatcgg tttattcact acaccctgcc cacgctcctc
    43561 acgccattcg cgcatatccg taatgtcgat gagcgcaccg tcagtcacgg cctgcacggt
    43621 ttcacgctgg cttccggcac ggcgatgcat acaggtggtg atctgcaaag gaccggccga
    43681 aaaatggtgc tcggcaaaca gcatttcgcc agcgtcatta gtcagtagcg taccgccatc
    43741 cagagaggct ttaccgcccg acaaccacag cgcggtatcc accacatgca gataatcatc
    43801 cagcaacgtg aaataaagat cgtgtggccc gacgctattg ctacgatgtt tatccattct
    43861 tagcgaggct gcggtggcga gttgcttttt taactcaccg tagagtggtg cgaaacgacg
    43921 gttaaaaccg accatcaacg tcagtttttt ccgcgccgcc agttccacca gccgttcagc
    43981 atcgcgcaga ttttctgcca gcggtttatc gacacagaca tgtacccccg cattgagtaa
    44041 cgtactgacc acgtcaaagt ggctggcggt gctggaatgc acaaaaaccg catcgcaact
    44101 ggcggcaagg ctggataacg aatcggcata aggaatgcgc cagctgtcac aaattggcag
    44161 ggctttcgcg cgcgtaggcg accacgcacc ttgtaacgtc cagtcagacg ctgccgccag
    44221 caccggtaac cacgcctttt gcgcgatgcc gcctaacccc actacgccga tacgtaattt
    44281 tttcacttta atctcccaga tgggccagca acgaatcaag acgctgtttc agttctgcca
    44341 cttcgatttc cagagcttcg acgcgggcct gtaaatcacc gtcaaccgcg ttcgacatag
    44401 ccgttaccac cggctgatct tcaacctcac cactgaaaag atgcatgtag cggctttcgc
    44461 gtttacccgg ttcgcgggcc agacgcacca caaaaggacc atcttcgcga ttcgccagtt
    44521 gttccagcgt cgactcaact tccgccatat cgctgaattc atacattcgc gcggcgcggc
    44581 tgcgcagttc acccggcgtc tgggcaccac gcaataacaa cgtggtgatt aacgccactt
    44641 ctgcggcgct cagtttcaga tcgccaaatt ctgaattaca aaaacgttgc tcatatttgg
    44701 tgacccgatt accaaaaccg ctcaccgtgc gtagataatg acgtttgacc agattatcca
    44761 gctgttcctg cacttcagat tcactcagat tcatgaccgg ttcacggttc gttttctgat
    44821 tacaggccgt gactacacca ttgactgaga gcgggtattg ctccggcgtg gtcacctgct
    44881 tttccagcaa gcaaccaatc acgcgagctt caagggcagt aagttgatat ttcatgagat
    44941 tctccttagc ggccgggcgt ccagtctggg gtagttaatg ccgtcagtac gtgatcgcgc
    45001 cattgtccat caatcaacag atagtctttc gcatagcctt ctttttcaaa acccagtcgc
    45061 gccagtaaat caccgctgcg tttattgtgc ggcatataat tagccataat gcgatgaata
    45121 tgttgggtgc gctgcatata acgaatggct gcggtcaggg cttcaaacat gagtcctttg
    45181 ccctgccatt tttgcccaat cgaataaccg agatagcagg catgaaaaga gccacgaaca
    45241 acattggaaa aattggcaac gccaataatc tctttttcgt ccgggtcgaa taagccaaag
    45301 tagaaagctg aaccttgttt atgaaattcg ttaatcatcc ctagcctggc ctgccagcct
    45361 gatggataac agtggctttc gtcgcgcact ggctcccagg gcttgaggaa atggcgattc
    45421 tctgcgtaat aatccgcaag acgccaggca tcacgatcat gcaccagacg cacgaccagt
    45481 cggtctgtgg ttaagcgcac ttttggcacg ttactgcgat agccaaacat tctataccta
    45541 ctccttcccg taacgtcttt aatacgctgt aagtattact atacctgcgc aaccagaccc
    45601 tgtgaaaagt ccagaatgcc attttctgca ttattcacat atttgataaa aactctctat
    45661 caaagcgacg ttttgataaa aaaatattgt ccccgaagtg atgggaaaag tcttagcggc
    45721 aatgcagaat agaaagtgct catcttttat ttcccgggag gggaaatgtc ccgcgtgtcg
    45781 caggccagga acctgggtaa atatttcctg ctcatcgata atatgctggt cgtgctgggg
    45841 ttctttgttg tcttcccgct gatctctatc cgcttcgttg atcaaatggg ctgggccgcc
    45901 gtaatggtcg gtattgccct cggcctacgc cagtttattc agcaaggtct gggtattttc
    45961 ggcggagcaa ttgccgaccg ctttggtgcc aaaccgatga ttgttaccgg tatgctgatg
    46021 cgtgccgccg gattcgccac aatgggtatc gcccacgaac cgtggctact gtggttttca
    46081 tgcctgctct cgggactcgg cggcacgttg tttgatccgc cgcgttcggc gctggtggtg
    46141 aaactaatcc gtccacaaca gcgtggtcgt tttttctcgc tgttgatgat gcaggacagt
    46201 gccggtgcgg tcattggcgc attgttgggg agctggctgt tgcaatacga ctttcgcctg
    46261 gtctgcgcca caggggcagt tttgtttgtg ctgtgtgcag cgttcaatgc gtggttgtta
    46321 ccggcatgga aactctccac tgtacgcacg cccgttcgcg aaggcatgac ccgcgtgatg
    46381 cgtgacaagc gttttgtcac ctatgtcctg acgctggcgg gttactacat gctggctgta
    46441 caagtgatgc tgatgctgcc aattatggtc aacgacgtgg cgggcgcgcc ctctgccgtt
    46501 aaatggatgt atgccattga agcgtgtctg tcgttaacgt tgctctatcc tatcgcccgc
    46561 tggagtgaaa agcattttcg tctggaacat cggttgatgg ctgggctgtt gataatgtca
    46621 ttaagcatga tgccagtggg catggtcagc ggcctgcaac aacttttcac cctgatttgt
    46681 ctgttttata tcgggtcgat cattgccgag cctgcgcgtg aaaccttaag tgcttcgctg
    46741 gcagacgcaa gagctcgcgg cagctatatg ggatttagcc gtctgggtct ggcgattggc
    46801 ggcgctattg gttatatcgg tggcggctgg ctgtttgacc tgggcaaatc ggcgcaccag
    46861 ccagagcttc cgtggatgat gctgggcatt attggcatct tcactttcct tgcgctgggt
    46921 tggcagttta gtcagaaacg cgccgcgcgt cgtttgctgg aacgcgacgc ctgatttgat
    46981 actcccccct gactcctcca tactgaaagt gaactgacag ccaaatggag gagtcacgtg
    47041 aagctataca tttacgatca ctgcccttac tgcctcaaag cccgcatgat tttcggcctg
    47101 aaaaatatcc ccgtcgaatt acatgttctg ctcaacgacg acgcagaaac acccacccgg
    47161 atggtcggtc aaaaacaggt tcccattctg caaaaagatg acagccgcta tatgccagaa
    47221 agcatggaca tcgttcacta tgtcgataaa ctcgacggca aaccgttact gaccggcaaa
    47281 cgttcccctg ccattgaaga gtggctgcgc aaggtcaatg gctacgccaa caaactgctg
    47341 ttgccgcgtt ttgccaaatc ggcatttgat gagttttcta ctcctgccgc gcgcaaatat
    47401 ttcgtcgaca agaaagaggc cagcgcgggt aattttgccg acctgctggc ccactctgac
    47461 ggtctgatta agaatatcag cgatgattta cgtgcgctgg acaaactgat cgtcaaaccg
    47521 aacgccgtga atggcgaact ttcggaagat gatattcagc tatttccgct actgcgtaat
    47581 ctgacgctgg tagccggaat caactggcca agccgcgttg ctgattaccg cgataatatg
    47641 gcgaaacaga cacaaatcaa tttgttatca tcaatggcga tttaatcctg acccgccgcg
    47701 gcagcctgta ttcgcgggtt gccgccgttt tcagtttttt ctgctggcgc gtttatcgct
    47761 ggtgacacat gctgcgttgt gtcaggatat tgacgcagat ttcatgtcga ggactaccat
    47821 gaacaagttt ttatttgccg ctgcattgat cgtcagtggc ctgctcgttg gctgtaatca
    47881 actcacccaa tacaccatca ccgaacaaga aattaaccag tcgcttgcga aacataataa
    47941 tttctcaaaa gatatcggtt tacccggcgt ggctgacgcc catattgttc tgacaaacct
    48001 gaccagccaa attggtcgcg aagagccgaa taaggttacc ctaaccggag acgccaatct
    48061 ggacatgaac tccctgttcg gtagccagaa agcgaccatg aaactgaagc tgaaagcgct
    48121 gcccgtgttt gataaagaaa aaggtgcgat cttcctgaaa gagatggaag tggtcgatgc
    48181 gacggtacaa ccggaaaaaa tgcaaacggt gatgcaaacg ttgctcccct atttgaacca
    48241 ggcattacgc aattacttta accagcaacc tgcttacgtc ctgcgcgaag atggcagcca
    48301 gggcgaagca atggcgaaga aactggcgaa aggcattgaa gtgaagccag gcgaaattgt
    48361 cattccgttt actgattaat cacgagggcg cattcgcgcc ctttattttt cgtgcaaagg
    48421 aaaacgtttc cgcttatcct ttgtgtccgg caaaaacatc ccttcagccg gagcatagag
    48481 attaatgact gcaccatccc aggtattaaa gatccgccga ccagacgact ggcaccttca
    48541 cctccgcgat ggcggcatgt taaaaactgt cgtgccgtat accagcgaaa tttatggacg
    48601 ggctatcgta atgcccaatc tggctccgcc cgtgaccacc gttgaggctg ccgtggctta
    48661 tcgccagcgt attcttgacg ccgtacctgc cgggcacgat ttcaccccgc tgatgacctg
    48721 ttatttgaca gattcactag accctaatga gcttgagcgt ggatttaacg aaggcgtgtt
    48781 caccgctgcc aaactttacc cggcaaacgc aaccactaac tccagtcacg gcgtgacgtc
    48841 agttgacgca atcatgccgg tacttgagcg catggaaaaa atcggtatgc cgttactggt
    48901 gcatggtgaa gtgacacatg cagatatcga catttttgat cgtgaagcgc gctttattga
    48961 aagcgtgatg gaaccgctgc gccagcgcct gactgcgctg aaagtcgttt ttgagcacat
    49021 caccaccaaa gatgctgccg actatgtccg taacggaaat gaacggctgg ctgccaccat
    49081 cactccgcag catctgatgt ttaaccgcaa ccatatgctg gttggtggcg tgcgtccaca
    49141 tctgtattgt ctacccatcc tcaaacgcaa tattcaccaa caggcattgc gtgaactggt
    49201 cgccagcggt tttaatcgcg tattcctcgg tacggattct gcgccgcatg cacgtcatcg
    49261 caaagagagt agctgcggct gcgcgggctg cttcaatgcc ccaaccgcgc tgggcagtta
    49321 cgctaccgtc tttgaagaga tgaatgctct gcagcacttt gaagcattct gttctgtaaa
    49381 cggcccacag ttctatggct tgccggtcaa cgacacattc atcgaactgg tccgcgaaga
    49441 gcaacaggtt gctgaaagca tcgcattgac tgatgacaca ctggtgccat tcctcgccgg
    49501 ggaaacggta cgctggtccg ttaaacaata aaaatcctag cttcctgttg tcattaggtt
    49561 attttacctg tataaataac cagtatattc aacagggggc tattatgcga attgaagtca
    49621 ccatagcgaa aacttctcca ttgccagctg gggctattga cgccctggct ggcgaacttt
    49681 cccgccgtat tcagtatgcg tttcctgata atgaaggcca cgtatcggta cgttatgccg
    49741 cagcgaataa tttatcggtt attggcgcaa caaaagaaga taaacagcgc attagcgaaa
    49801 ttctccagga aacgtgggaa agcgccgatg actggtttgt cagcgaataa tatgcagtga
    49861 tttttgtttt tgccgggtcg ccccggcttt tttattttac ttacaaattt cgagtacaaa
    49921 atttctacac ttcctcaaaa taattgcaca atatgctaaa aaaatatgaa tgacatgctg
    49981 ttttattgtt catataaatc ttaaaagcaa aaatttatct gttcccccct ttaaaaattc
    50041 ggtgaatacc cttacttatt ggtatactga agttgcttca gataaaaacg cattgaacct
    50101 cgaataacgt tgtctagtaa cacgaattag ggggccatga tggaaaaaaa taatgaagtc
    50161 attcagactc atccgctcgt agggtgggac atcagcaccg ttgatagcta tgatgcgctg
    50221 atgttgcgtt tgcactacca gaccccaaat aagtccgagc aggaagggac tgaagttggt
    50281 cagacgctct ggttaaccac tgatgttgcc agacaattta tttcgatatt agaagcagga
    50341 atcgccaaaa ttgaatccgg tgatttcccg gtaaacgagt atcggcgtca ttaacatgac
    50401 atgaccatcc acccaaaagg cacctcaccg gtgcctttac catttctggg ttgtatatct
    50461 gcattcagtt aattactctg cttaaaccat gagagcagag agaaaaaaat gaaatacgat
    50521 ctcatcatta ttggcagcgg ttccgtaggc gctgccgccg ggtattatgc aacccgcgcc
    50581 ggtttaaacg tgctaatgac cgacgcccat atgccaccgc atcaacacgg cagccaccac
    50641 ggcgatacgc gattaattcg ccatgcttat ggtgaaggcg aaaagtatgt cccgctggtt
    50701 ctccgcgcgc aaacgctgtg ggatgaactc tcccgccaca acgaagaaga tcccattttt
    50761 gtacgctctg gcgtcattaa ccttggcccg gctgattcca catttctcgc caacgtcgcc
    50821 cacagcgccg aacaatggca actcaacgtt gaaaagctcg atgcgcaagg gattatggcc
    50881 cgctggccgg aaatacgcgt cccggacaac tacatcggct tatttgagac tgattccggt
    50941 tttttgcgca gcgaactggc gattaaaacc tggatccaac tggcgaagga agcgggctgt
    51001 gcgcagctgt tcaactgccc ggtcaccgca attcgtcatg acgatgatgg cgtaactatt
    51061 gaaacggctg acggtgagta tcaggcgaaa aaagcgattg tctgcgcggg aacatgggta
    51121 aaagacctgc tcccggagct gcctgtccag cccgtacgca aagtatttgc ctggtatcag
    51181 gccgatggtc gctatagcgt gaagaataaa ttcccggcgt ttaccggtga actgcccaat
    51241 ggcgatcaat attatggttt tccggcagaa aacgacgcgt tgaagattgg caaacataac
    51301 ggaggccagg ttatccattc agcggatgaa cgtgttccgt ttgcggaagt ggtcagcgat
    51361 ggttcggaag ccttcccgtt cttgcgcaat gtcttgccgg gtatcggttg ctgcctgtac
    51421 ggcgctgcct gcacctatga taattcgcct gacgaagatt ttattatcga tactctaccc
    51481 ggccacgata atacactgct cattaccggt ctgagcgggc acggttttaa atttgcgtca
    51541 gttttagggg aaatagctgc cgattttgcg caagacaaaa aaagcgattt tgatttgacg
    51601 ccattcaggc tttcccgctt ccaataatca tcattacggc ctccagaatg aggccgtttt
    51661 tacaggagta tttaatgcgt cggctgttgc actatctcat caataatatt cgcgagcatc
    51721 tgatgcttta tctttttctc tggggattgt tggcaattat ggatctgatc tatgtgtttt
    51781 atttttagtt ttccgcactg acataaactg acaaatattt aatctccctt tcatttagcg
    51841 tgaaaaattg aatgagatta ttcctgcttc tacagcactc accagacatc tctgatgctg
    51901 aacccactaa acttatgaag attctttcat tattaattca taattattga aataaaaata
    51961 aatatcattc aatttgttat ttttgtgttg caattcattc tccgacacgc cattttcttt
    52021 ctctcgcaaa ttcgttttgg atgcacttat gtcattcaca aatacccctg aacgctatgg
    52081 tgttatatct gcggccttcc attggctaag cgcaattatt gtctacggca tgtttgcttt
    52141 aggtctgtgg atggtcacgc tcagttatta cgatggctgg tatcacaaag cacccgaact
    52201 gcataaaagc atcggtattt tgttaatgat ggggctggtt attcgcgttc tgtggcgcgt
    52261 catatctcca ccgccgggtc cgctgcagag ttattcgccc atgactcgtc ttggcgcaaa
    52321 ggctggacat ctcgcattgt acttattgct gttcgccatc ggcatcagcg gctatctgat
    52381 ctcaactgcc gatggtaaac cgatcagcgt ttttggctgg tttgacgtcc ccgcgaccct
    52441 cgccgacgct ggcgcacagg ctgacttcgc cggtgccctg catttttggc tcgcgtggag
    52501 cgtcgtagta ctgtccgtta tgcatggatt tatggccctg aagcatcatt tcatcgataa
    52561 agacgacact ctgaagcgca tgctgggaaa gtcgtcatct gactatggag tataaaaaat
    52621 gaaaaaaagc ctgcttggtt taaccttcgc ttccctgatg ttctctgccg gttcagcggt
    52681 tgccgccgat tacaaaattg acaaagaagg tcagcacgcc tttgttaact tccgcatcca
    52741 gcatcttggc tatagctggt tatatggcac ctttaaagat ttcgacggta cttttacctt
    52801 tgatgaaaaa aatccggctg ccgataaagt gaatgtgaca attaacacca ccagcgtcga
    52861 tactaatcac gccgaacgcg ataaacatct tcgcagtgca gatttcctca atactgcaaa
    52921 atatccactg gcaacattca cctccaccag cgtgaagaaa gacggtgacg acctggatat
    52981 taccggcgat ctgacgctga atggcgtaac caaacccgtc acgctggaag cgaaattaat
    53041 tggtcaaggc gacgacccgt ggggtggtaa acgtgcaggc ttcgaggccg aaggcaaaat
    53101 taagctcaag gacttcaata tcaaaacaga tttaggtcca gcttctcagg aagtggatct
    53161 gattatttca gtggaaggcg tacagcagaa gtaagcgtga aatgttgccg gatggcacgc
    53221 atccggcaat gatatttatt ctgttggatc aggaatgccc agtgttgtat tcagacgtcc
    53281 acgagactta ttaaagatct tattgccatt ttcacgtcct gcccgacggc gtcgctgttc
    53341 ttcaggcggt aacgcgcttt cttcgcagca aatctcacta caacaacctt tgtatttttc
    53401 cgcacatact ggacactgaa taaaaagcag gtggcagcca tcatttttac agttggtatg
    53461 gctgtcgcac ggcgcaccgc actggtggca atgcgcgata atctcatccg atatacgttc
    53521 gcccatccgc tcgtcaaaaa caaaattttt gccaataaaa cgtaccggta aaccctgctc
    53581 gcgcgccttg cgggcgtatt caataattcc gccctcgata tgccagactt tattgaatcc
    53641 gttatgtttc atccaggcac tggccttttc acaacgaatg ccgccggtgc agtacataac
    53701 gattttttta tctttatgtg cctgcatcat ctcgactgct tttggcagct gctcacggaa
    53761 ggtatctgcc ggaatttcca gcgcgttttc aaagtgcccc acttcatact catagtggtt
    53821 acgcatgtcg ataaacaatg catcgggatc gtcaagcatg gcgttcactt ccgccgcttg
    53881 cagatactca cccacattgc tggcatcaaa gtgaggatcg tcaataccgt cggcaacgat
    53941 acgatcgcgg accttcatgc gcagtaccca gaaggatttc ccgtcatcat ccaacgcgat
    54001 attcaggcgt aagccctcta aagccgggtc gaaggcataa agctgcgcgc gaaatgtttc
    54061 aacattgctc gcaggtacgc tgatttgcgc gttaatgccc tcatgcgcca gatacactcg
    54121 cccaaaaaca tccagcgcgg taaacagctg atataaagcg tcacgggtcg ccttaggatc
    54181 ggcgatgtgg aaatacttgt aaaacgaaat ggtggttcgc ggttcgctct cagccaacat
    54241 tttggctttt aacgcgtcgt tggaaatgcg gttgtgtaac actggcatgg tgtacggttc
    54301 ctgcgagatg ggaaagtaaa aatccgcggc atgatatagc aattatcgat aattaacatc
    54361 cacacatttt acgctacatt tgcgcattaa aaattatttg ttatttacaa gcgcggcaat
    54421 ttcgcccagt cttcagccac aaattttggt tgcgggcgaa aaaatgcgac aatacataca
    54481 attgcccgta taggttgaaa aacaggattg atatgacgaa tctacccaag ttctccaccg
    54541 cactgcttca tccgcgttat tggttaacct ggttgggtat tggcgtactt tggttagtcg
    54601 tgcaattgcc ctacccggtt atctaccgcc tcggttgtgg attaggaaaa cttgcgttac
    54661 gttttatgaa acgacgcgca aaaattgtgc atcgcaacct ggaactgtgc ttcccggaaa
    54721 tgagcgaaca agaacgccgt aaaatggtgg tgaagaattt cgaatccgtt ggcatgggac
    54781 tgatggaaac tggcatggcg tggttctggc cggaccgcag aatcgcccgc tggacggaag
    54841 tgatcggcat ggaacacatt cgtgacgtgc aggcgcaaaa acgcggcatc ctgttagttg
    54901 gcatccattt tctgacactg gagctgggtg cgcggcagtt tggtatgcag gaaccgggga
    54961 ttggcgttta ccgcccgaac gataatccac tgattgactg gctacaaacc tggggccgtt
    55021 tgcgctcaaa taaatcgatg ctcgaccgca aagatttaaa aggcatgatt aaagccctga
    55081 aaaaaggcga agtggtctgg tacgcaccgg atcatgatta cggcccgcgc tcaagcgttt
    55141 tcgtcccgtt gtttgccgtt gagcaggctg cgaccacgac cggaacctgg atgctggcac
    55201 ggatgtccgg cgcatgtctg gtgcccttcg ttccacgccg taagccagat ggcaaagggt
    55261 atcagttgat tatgctgccg ccagagtgtt ctccgccgct ggatgatgcc gaaaccaccg
    55321 ccgcgtggat gaacaaagtg gtcgaaaaat gcatcatgat ggcaccagag cagtatatgt
    55381 ggttacaccg tcgctttaaa acacgcccgg aaggcgttcc ttcacgctat taaatctccc
    55441 atgccggatg cttcagaagg gtatccggca ttaccacagc aaatccccct gatttagcga
    55501 taaaagctct ctggattgcg ccccctggaa gtcgggcgca taattagtgt gcttatcttt
    55561 tcttcttatg ttcaccgcgc ctggcgcacc aacagcggat tgctatgtca ccctgtgaaa
    55621 atgacacccc tataaactgg aaacgaaacc tgatcgtcgc ctggctaggc tgttttctta
    55681 cgggtgccgc cttcagcctg gtaatgccct tcttacccct ctacgttgag cagcttggcg
    55741 ttacgggcca ctccgccttg aatatgtggt ccggtattgt cttcagcatt acatttttat
    55801 tttctgccat cgcctcaccg ttttggggtg gactcgccga ccgtaaaggc cgcaaactca
    55861 tgctattacg ctctgccctc ggcatgggca tcgtgatggt gttgatgggg ctggcacaaa
    55921 atatctggca gtttttgatc ctacgggcgc ttcttgggtt acttggcgga tttgtcccca
    55981 acgctaatgc tcttatcgcc acacaagtac cgcgtaataa aagcggctgg gcgctgggta
    56041 cgctctccac aggcggcgtt agtggtgcgt tgctcggccc aatggctggc ggcctgctcg
    56101 ccgatagcta cggcttacgt ccggtattct ttattaccgc cagtgtgctc atactctgct
    56161 ttttcgtcac cctgttttgc atcagagaaa aattccagcc ggtcagcaaa aaagagatgc
    56221 tgcacatgcg tgaagtggtg acatcactta aaaacccgaa actggtactc agcctgtttg
    56281 tcactacgtt aatcatccag gtggcaacgg gctcaattgc ccccattctg acgctgtatg
    56341 tccgcgaact ggcgggtaac gtcagtaacg tcgcctttat cagtggcatg atcgcctcgg
    56401 tgccaggcgt ggcggctctg ctaagtgcac cacgactcgg caaacttggc gatcgaatcg
    56461 gacccgaaaa gatcctgatt acggcgctga tcttttctgt actgctgttg atcccaatgt
    56521 cttacgttca gacgccatta caacttggga ttttacgttt tttgctcggt gccgccgatg
    56581 gtgcactact ccccgccgta cagacactgt tggtttacaa ctcgagcaac caaatcgccg
    56641 ggcgtatctt cagctataac cagtcgtttc gtgatattgg aaacgttacc ggaccattga
    56701 tgggagccgc gatttcagcg aactacggtt tcagagcggt atttctcgtc accgctggcg
    56761 tagtgttatt caacgcagtc tattcatgga acagtctacg tcgtcgtcga ataccccagg
    56821 tatcgaactg atttttcgcc tttcatactt gtaaaagcgg agaatcagct atccttttcc
    56881 ctgaaacctc atcaactcaa agggagaatc gtgatgacca tgtacgcaac gcttgaagaa
    56941 gccattgacg ctgcacgcga agaatttctt gcagacaacc ccggcatcga cgccgaagat
    57001 gcgaatgtgc aacagttcaa tgcccaaaaa tacgttttgc aggacggcga catcatgtgg
    57061 caagttgagt tttttgccga cgaaggggaa gaaggtgaat gtttacctat gcttagcggt
    57121 gaagccgcgc aaagtgtctt tgatggcgac tatgatgaga tagagatccg ccaggagtgg
    57181 caggaagaga atacattaca tgaatggggc gagggggaat ttcagcttga gccaccgctg
    57241 gataccgagg aaggacgcgc agcagctgat gagtgggatg aacgttaatt actcatacgg
    57301 gccatgatga atgtcgatcg gcagcagtaa agtatcaaaa accagggaga atggcagatc
    57361 aaggattgtg acataacgcc aggcggagtc acgcacatcc cattgcacac cgggataata
    57421 ttggttgcca tgcccctgcc ccggtatagt gcggctaatg atgctgccac agccactcag
    57481 caaggtcacc atgatgctca ccacaattaa acgcatttga tacctcttcc gtaaaaaaat
    57541 accggcataa cgccggtatt tttattggct cgttgttttt gccagatgtg gcgtgaacgc
    57601 cttatccgac ctacaaaatc ttgcaaaatc aacacattgc aggagcgatg taggcctgat
    57661 aagcgtagcg catcaggcaa ctacgttttt attgcgaagc cgcatccggt ttacgcaatg
    57721 ccagtggatt gagctttatc ccttcgtaat aactcaccca tgaagaatat ctctcagggg
    57781 aattccagac gcggaaatgc agacgggcca tcgtcaccgg atcgcttagc agcaccaggc
    57841 gacgatcgcg attcagcttc tctggcgtct cgttcagcgc ctgttcaacg tggcggtcac
    57901 gggcgatttc cagcacctta ctggcgcggt gacgcgcggt cgccattgcg gttgccagag
    57961 cgttaaatga cgggttaaac actgcgtgca taaagccatc gtcaagggag cgttggcgat
    58021 tcatctcaag gaaccgatcg gtatcaacca gcacctgcgg cggtgaatac tcttccggga
    58081 tcaggaacag tttccagctt ttggtgcgca gaccaacggt ggcacggctg gaaatcaccg
    58141 aaacaaacgg tgacaggatc aacgagaaga caatcggtgc cagccagaac aggaaacgca
    58201 gatccaacca cgccatccca acagcccaca ctaaccctaa cagcagctgt gagccgtggc
    58261 gtttgaacgc ttcaccccag gaagtggagt catcatcacg ctgcggtgaa ttccacacca
    58321 cttcccagcc aaggaacgcg ctgacgacga agaccgtatg gaacagcatg cgtaccggag
    58381 ccagcagcac ggaaaacagc acttccagca gcaacgataa tgtaacgcgc cagaagccgc
    58441 catattcttt cgttcctttg caccaaataa gcaaaatgct caatagcttt ggcaggaaca
    58501 acagcaccat ggtcgaagca aaaagtgcaa tcgccagctc aggacgccac tgcggccaca
    58561 ccgggaacaa ctgccgtggt tgcaggaagt attgcggttc ggtcaacgca tgcactacct
    58621 gcaatgcagt agagagcgcg aggaacataa accacagcgg agcggagaga taagacatca
    58681 cgcccgtcag aaataccgca cggtgaaccg ggtgcatacc cttcaccagg aacagacgga
    58741 agttcatcag gttaccgtgg caccagcggc ggtcacgttt tagctcatca agcaagttag
    58801 gcggtaattc ttcataagaa cccgggagat cgtaagcaat ccagactccc caacctgcac
    58861 ggcgcatcaa cgcagcttcc acgaagtcat gtgacaggat tgaaccggca aaggaacctt
    58921 cgcccggcag cggagccagt gcgcagtgct cgataaacgg tttcacgcgg ataatcgcgt
    58981 tatgtcccca gtagtgcgac tcgccaagtt gccagaagtg caaaccggct gtaaacagtg
    59041 gcccatacac gcgagtcgcg aactgctgac agcgtgcata cagcgtatcc ataccggacg
    59101 ctttcggcga cgactgaatg atcccggcgt tcgggttggc ttccatcaaa cgcaccaggc
    59161 cgcataaaca atcaccggtc attaccgagt cagcatccag caccaccatg tagctgtact
    59221 ggctgcccca gcgacggcag aagtcatcga tattaccgct tttacgcttc acgcggcgac
    59281 ggcggcggcg atagaaaatc tgaccttcgc caccgacttc agcgataagc tccatccagg
    59341 ctttttgctc tgcgacgcag atatccgggt tataactgtc actcagaatg tagacatcaa
    59401 agtgtttggc attcccggtg gcttttactg attcccacgt tgcacgcaag ccagcaaaaa
    59461 cacggttcac gtcttcgtta cagataggca tgatcaacgc cgtgcgatgc tccgggttta
    59521 atggttcatc gccaactgtt gacgcagata tactgtattt atcgcgacca ataagcagtt
    59581 gcaggaaccc catcaacgcc gtccagaatc cggcggacac ccaacagaac agtaccgcaa
    59641 agaggatcag gataccggtt tgcagcatat aaggcagaag ctgcataaag gaaacccaca
    59701 aatcctgacc aaccatatcc ataggattaa tcagcgccca accctgataa ggaagaatgg
    59761 tcttcatata ccaggtcgcg acgacagttt gcgcgagcgt caggatcaac agaatgtaac
    59821 ggcggatggt accgacggta cgccactttt gctcgctctc ctgctcttct ttggtcaaac
    59881 gagccagata gcgcggcgtt acatcgcgtc cacgcaggcg atcccagaaa cggcctaccg
    59941 ggttggtacg ccacgggtcg ggaaacatcg aggagcgttt tgcttctggc atcgccttaa
    60001 gctgatcgcg cccttcgtcg tctttaatta actgtccatc agcaagtgaa tctggccagg
    60061 cttgttccag acgcgccttt accgagcctt gcggggagtc atcctcccgc gcccaggtac
    60121 ggtggtcggc atccagcgcc tgatgaacgg cgcggatatc agtcttcggc aatgccgctt
    60181 tctcgcttgc ggcgatgggc attgcgtcaa tgtactcagt tgtcttattc attggcaggt
    60241 aactggtagc tccaggtttc actcaacgtc tgatcggcat tcaccagcgc agcacgcatt
    60301 tcagtggttt tcttggcatc tttcactttc acacgcatca ccagacgcca gcctttggta
    60361 accgggttat agcgcaccgt gctttcaact atctcaccat tatcaccaat gctggtttgc
    60421 gctgtgaccg gggtatcctc tggcagtttt ttcatctctg cgccggtaaa atcgaccaca
    60481 aaggcgatag taccgtcagg ctggcgaatc aggttcgact gcttcacatc ccccgttgaa
    60541 cgacgcgttt gttgcaccca tgcgttatct ggcgcatgca gtttgtcttc atcacggctg
    60601 aaggtgatgg tgtatttaaa gttcatctct ttacccggct ccggcagctg atccggcgtc
    60661 cagtaagcga cgatgttatc gttggtttca tcgttggttg gaatttccac cagctcaacg
    60721 ctgcctttgc cccactcccc tttcggagtc acccatgcgc ttggacgaag atcgtaacga
    60781 tcatcgagat cttcaaagcg ggagaaatca cgaccgcgct gcaacagacc gaagccttgc
    60841 gggttttcca tggagaagct gctgaccgct aaatgtttcg ggttattcaa cggacgccag
    60901 atccattcgc cattaccagc atggatagag agaccgttag agtcgtgcaa ctccggacga
    60961 tagttatttg caggcgacgg ttggttcggc ccaaacagga acatactggt taacggtgca
    61021 acccccagtt tgccgacttt atcgcgcaga tagattttcg actgcacatc cacaaccgtg
    61081 tcacgccctg gcataactac gaatttgtaa gcacctgtcg cgcgcggcga gtcaagcaat
    61141 gcataaatgg ttaaacgttt atcagtcggt tttggacgct cgatccagaa ctctttgaag
    61201 cgtggaaatt cttcacccga tggcaaggcg gtatcaattg ccaggccgcg tgcagaaagg
    61261 ccataaacct gacctgcacc aatcacgcgg aaatagctgg ccccgagcat gctgacgatt
    61321 tcatcgtttt tatctttgct gttgatcggg taaagcactt taaaaccggc aaaaccaagg
    61381 tcttttaccg tgtctttgtc atgctgaaca tcgccgaaag tgaaataatc cgggctgtat
    61441 ttgattcgtt tgactgcggt ggcagtcact tcatttattt tgaccggggt atcgaagtac
    61501 ataccctgat ggtagaactc gagtttgaat ggggtcttca gattgttcca gtacgctttg
    61561 tcatgattaa actggatctg ctgatagtcc gcgtatttca tgtcgcggaa aacggagggc
    61621 aagttgcttt tgggcgcatc atagcctttc ccggctaagg attgagcttg ctttgcgaca
    61681 tcatcaatac tgaaagccca gcttgaagat gtatacaggg ttaacattac tgcagcactc
    61741 aaccaacgca ttttcatcat ttgtagttta tgtttcataa ttagtaagca cttccccctt
    61801 tgtgtgctta tatcgatccg atctatttta atggataacc agccattccg acaacagaat
    61861 cccggtatcg ttcagcacgc tggctcatgg attaagcacc tgattgcaac cgttttcact
    61921 ttccgtgctt ctcctaaaga cagaagcatc gacttcgtgt agggtttgcc agcaaattta
    61981 tttatcaacc gtcttacaga cgcttccatt ctggttagtg gtagcgatga tgctatgcat
    62041 aaaaaccttg aagcctgcgc ctggcgcggt caggtcggtg tcgctatggc aacaagccta
    62101 aaggcataac ccgacaggga taagacgaaa aatccgagat tagttaacca tatatgaatc
    62161 cagtacccgc gcaacgtgaa tatttcctcg actccatccg cgcctggctg atgttgttag
    62221 ggatcccttt tcatatttct ttaatctatt cgagccatac atggcatgtg aatagcgccg
    62281 aaccgtcatt atggctgacc ctttttaatg acttcatcca ctcgttccgc atgcaggtat
    62341 ttttcgttat atccgggtac ttttcctaca tgcttttttt acgctatccc ttgaaaaaat
    62401 ggtggaaagt acgtgtcgaa cgtgtaggta tcccgatgtt aacagccatc cccctactga
    62461 cattgccgca atttattatg ctgcaatacg tcaaagggaa agcggaaagt tggcctggac
    62521 tgtcattgta tgacaaatat aatacgttgg cctgggaatt aatatcacac ctgtggtttt
    62581 tactggtgtt agtggtcatg acgacgctgt gcgtctggat attcaaacgt atcagaaata
    62641 atttagaaaa ttctgataaa acgaataaaa aattctcgat ggtaaaacta tcggtgattt
    62701 ttttgtgcct cggcatcggt tatgcggtaa taagaagaac aatttttatt gtgtatccgc
    62761 ccattctgag taacggcatg ttcaatttta ttgtcatgca aacgctattt tatttgccgt
    62821 tctttatcct cggcgcactg gctttcattt tccctcatct taaagccttg tttaccacgc
    62881 cgtctcgtgg ctgtaccttc gcagcagcat tggcatttgt cgcttattta ctcaaccagc
    62941 gctatggcag tggcgatgcc tggatgtacg aaaccgagtc ggtgatcacc atggtcctcg
    63001 gtctgtggat ggtgaatgtg gtcttctcct tcggccaccg tttgcttaac ttccagtcag
    63061 cgcgggtgac ttactttgtt aatgcatcgc tgtttatcta tctggttcac cacccgttaa
    63121 cgctgttttt cggcgcgtac attacaccgc acatcacatc caactggctt ggttttctct
    63181 gtggcctgat attcgtagta gggattgcga taattctgta tgaaattcat ttacgcatcc
    63241 cgttactgaa gtttttgttc tctggtaaac cggttgttaa gcgtgagaac gataaagcac
    63301 cagcccgtta agccgcgttt ataataacca ttccacaggc aatatcgacg ccagtctgac
    63361 cataacccgc ttccagaaac tggtggcggg ttcttttttg agaacaattt ctttctttgc
    63421 atgacgatca acccagttga tccgtcccca cctgtccaga cggagctgcc aggccgcatc
    63481 atactggctc tgaataaagc gtttatcaat taactgtgcc agcgtttcgc tctctatcac
    63541 gaaccccatt tcagtattga gcaatgtcga acgcggatcg aaattgaaag agccgataaa
    63601 caccgtttta ccatcgatgc taaaggtttt agcatgcagg ctggcaccgg aattaccggt
    63661 tatgccgcga tcgtgtaacg tactactttg ttcacgcgtc ggcttgagtt catataattc
    63721 cacgccgtag cggagcaatt ttttgcgcca gcgcgcgtat ccggcatgga cgacagcaac
    63781 atcgttagcg gcaagagaat tggttaggat cgcaatcttt accccttttc tcaccatccg
    63841 taagagttgc gccacacctg cgcgtgtcgg tacaaaatag gaagagataa tatcgatgcg
    63901 ttcactgggt gagcccatga tatcgaacag gcgctgcggt aacagtgaat gccgttttgc
    63961 cttgccctcc cctttcgccg gatcatcact taataaacgt gttttcgccc agataagcgg
    64021 caacgttcca tcaaccagat gatttataaa cgggctggat tccattttgc gtaaataacg
    64081 atgcgtcatg gcatcgttgt gccaggatgc tggtaactcg atgcgatccg ccatttcacc
    64141 ttccgggaca tccagcacct gctgtagggg tgaaaccgat ttgcaatacc agtagcgggc
    64201 gaaatcatcg gcaacgtcct ctacaactgg tcctattgcc atgacatcta aatccgaaaa
    64261 aagtggctct tcccctgcac caaaataggc atcaccaata tttcgccccc ctaccagcgt
    64321 caccacgcca tcaacagtga aacttttatt gtgcatacgg cgattgagac gggaaaagtc
    64381 ggtgatataa ccaagcggac gtagcaagcg aaacgagaaa ggattaaaaa gccggacttc
    64441 aatgcgggga tgactgtcaa gcaagcgtaa aatgtcgtca agtccgggtg tattgttgtc
    64501 gtccagcaac aaacggacgc gaacgccacg ctttgctgcg gctaacaggg cggaaaagag
    64561 taaccgaccc gacatatcgt cctgccagat gtaatactga acatccagcg tatgctctgc
    64621 catttcggcc agacgatacc gggcggcaaa cgcatccaga cttttctcca gagggaaaag
    64681 gccacactga ccgggatgtt gcgaacacag tggcagcacc gcgctcgcca gccggggcaa
    64741 atcattcatc tccttgttgg gtaaggagtc tttcgtagag gtgggtgttt tcttcatcat
    64801 aacagacaaa atatacctgt tcgggtaaag cgtgacgggt aataaattct gaaacggttt
    64861 ttactgcgat ttcagccgct gccgcacgag ggtaaccata aaccccagta ctgattgcag
    64921 gaaatgccac tgacgtatag ctgtttgccg ccaccagtcg cagactattg agataggcat
    64981 cctgcaaaag ctggtcttcg ttttgttcac caccacgcca gactggcccg acggtgtgca
    65041 ctacggcttt agcgggaaga tcgcctgcaa gcgtaataac ggcatgcccc gtagggcaat
    65101 cgccctgctg ttgcctgact tttaaacagg catccagcag agccggaccc gctgcgagat
    65161 gaatggcccc atcgacgccg ccgcctccca ttaatgacgg attagccgca ttcacaatca
    65221 catcaacggc cagtttggta atatcaccct gcacaacatg aatacgcgtt ttcataacgc
    65281 ctccttacac cttcttgttt cttaagtgta cagcaggctt tttggttaaa tcgcattttt
    65341 ctgctcagca aggtgagttg ttcagaaact tagttatatt cgatgttcat tacagccagc
    65401 tttttctgtt tatcaagata ctgcactttt actgaggcta tttgcgcgtt tttgacattg
    65461 ccgctcatta acgctttgct ggaataggtt ctgcccggaa cagagccatt ctgtgggcag
    65521 ttcatttcta cgggtgatga ctgggtgctg acgtcacatg ctggttccac aatttggcca
    65581 taaaaatgga tcacgccccc ttgcgcgctg cttcctgcga tggcaataaa aggaaaaaac
    65641 aaactgccga gcataagaga gtttaaaaaa ggtctgaaca tttattctat ttcctcaatg
    65701 attagtcatc cttgagggtt gtgttatcca tactttcagg ttggctgatc cgcagcagac
    65761 cattctctcc agattcatct tatgctcgat atttcaacaa attaagactt ttctgaagag
    65821 ggcggccatt gttgtgataa atgaagtgac tgtccatcag aaacagtaac aactattttc
    65881 acccgatcgt ccggggaaat atttaaactc aacttcgtca aagcaatggg ttgattagca
    65941 ggcaatgaga gggtcttttc ttgcttcgtc tgactttgcc ctgaactgcc ttcgcgcagg
    66001 gacaatattt gtactctgca cagacaagat tgagtaagag tgacttcagg aataatggtg
    66061 tacatatccc cttgctgggt cgtattaaag gttatctgac tggaaagtgc cgcaaggagt
    66121 aataacgcat tcatattctt ctcccgaaaa aaaacagggc ttgcgccctg tttctttaat
    66181 acagatgtgt attagtactg atgagcggtc gcgttgttac caaagccaac ctgagtgacg
    66241 ttaacgctgg agttagatgc agtctggtca acagcagcac cgttaccgcc accgaactgt
    66301 ttaacagtca tagtagaatc tttaccattc cactgatcaa gagtagcgct gttgccgaaa
    66361 ccacgctgag tcagatcgat tgagctgtca tcagaacctt ggccaacatc tgcgccgtta
    66421 ccgccgccgt gctgggtaat ggtcagatca gagttacggg cgtcagcttg cagagcaagt
    66481 gcagagttac caccaccgta ctggtaaatg ttcagctctg aatttgggcc gctattatta
    66541 ccgccaccac cgtggttacc gccgccgccg tactgaggaa caacacctgc cagagcgcta
    66601 ccggagaata cgattgctgc aattgctgct acttttaaaa gtttcatgta aaacccccat
    66661 cggattgatt taaaagtcga atagtaatta acgttgtgtc acgcgaattg ccatttgcga
    66721 ctgtctctgc actacaattg ccgttttttg agtaccatac tgtgtaatat ttgctttatt
    66781 accagaacct ttctggataa tcatcgcagt attaccataa gcaccttgcg aaatactggc
    66841 atcgttggca ctgcccgcct gatcaatata tgcaaggtta taatctcctg tctggtcaat
    66901 ctttgcccga ttgctactac cttcttgcgc aacaaccgcc aaaagttttg agcctccctg
    66961 ccgtaactga gcactattat tagtcccagc ttgaccaatt atggctgcct gattaaatga
    67021 agacttactc aattcattta ccgcgaagtt atattctgaa ttagctaaat cataacctgc
    67081 tgcggctgca atcccaggcg cacccagtat tgttaacatc ataaataaca atttgttttt
    67141 catgttgtca ccctggacct ggtcgtacac ttaagaaatt aaatcatttc aacttggttg
    67201 ttaacgcaac ctgtattttg ttaacgctgc gttacgatgg aaagtatgtc tgcggaaata
    67261 tttttaataa ctcacccgcg cgttgtattt ttcttttttt gttcatacca aagtattaaa
    67321 acccacgtaa atacgctgtt atctacgcaa aaaaatattt tgttttcttt taaatctccg
    67381 ttttccgcaa tcaaaaatca ccagacagcc attcttcttg cccgtcgctg attgctgcga
    67441 tatgtcttgc gcacaagccg tgacaaacct cgtctttttg cagtaaaaaa ttgtccacgg
    67501 aggtgtgaag aaaaaacaag aacgttttac atgacgaaag gact
//
LOCUS       DACBVI010000005        66886 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.005, whole genome shotgun sequence.
ACCESSION   DACBVI010000005 DACBVI010000000
VERSION     DACBVI010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.005"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(46..1014)
                     /gene="astE"
                     /locus_tag="IU325_01690"
     CDS             complement(46..1014)
                     /gene="astE"
                     /locus_tag="IU325_01690"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HAP5807747.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQTPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1007..2350)
                     /gene="astB"
                     /locus_tag="IU325_01695"
     CDS             complement(1007..2350)
                     /gene="astB"
                     /locus_tag="IU325_01695"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HAP5807748.1"
                     /translation="MNAWEINFDGLVGLTHHYAGLSFGNEASTSHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATVAPSTDTLDGKVHLTVANLNNKFHRSLEAPVSESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQVQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(2347..3825)
                     /gene="astD"
                     /locus_tag="IU325_01700"
     CDS             complement(2347..3825)
                     /gene="astD"
                     /locus_tag="IU325_01700"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HAP5807749.1"
                     /translation="MTLWINGDWVTGQGALRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFGDRQVRVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGKWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGRRTLLAPRLLQAGTSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLSCGLVSPAREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(3822..4856)
                     /gene="astA"
                     /locus_tag="IU325_01705"
     CDS             complement(3822..4856)
                     /gene="astA"
                     /locus_tag="IU325_01705"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HAP5807750.1"
                     /translation="MMVIRPVERSDISALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPEWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(4853..6073)
                     /gene="astC"
                     /locus_tag="IU325_01710"
     CDS             complement(4853..6073)
                     /gene="astC"
                     /locus_tag="IU325_01710"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HAP5807751.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLTDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDILHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLHGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLDLINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            6519..7325
                     /gene="xthA"
                     /locus_tag="IU325_01715"
     CDS             6519..7325
                     /gene="xthA"
                     /locus_tag="IU325_01715"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP5807752.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSTLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            7492..8202
                     /locus_tag="IU325_01720"
     CDS             7492..8202
                     /locus_tag="IU325_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP5807753.1"
                     /translation="MNAERKFLFACLIFALLIYAIHAFGLFELLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            8207..8884
                     /gene="ydjY"
                     /locus_tag="IU325_01725"
     CDS             8207..8884
                     /gene="ydjY"
                     /locus_tag="IU325_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HAP5807754.1"
                     /translation="MLQHYSASWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVAGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            8899..9606
                     /locus_tag="IU325_01730"
     CDS             8899..9606
                     /locus_tag="IU325_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP5807755.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGALWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLNSMDGFFTRYGKHTILVCRLLPFVPF
                     DPVSYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSILTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            9606..10154
                     /locus_tag="IU325_01735"
     CDS             9606..10154
                     /locus_tag="IU325_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP5807756.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10164..11330
                     /locus_tag="IU325_01740"
     CDS             10164..11330
                     /locus_tag="IU325_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP5807757.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPTALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFSEGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            11303..12838
                     /locus_tag="IU325_01745"
     CDS             11303..12838
                     /locus_tag="IU325_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP5807758.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQTL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWRSWRRTIPRVDGVRKP
                     ATPLLPGNTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVAAFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPALIVFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            12838..13491
                     /locus_tag="IU325_01750"
     CDS             12838..13491
                     /locus_tag="IU325_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP5807759.1"
                     /translation="MLCVKNVSLRLPESCLLTNVNFTVDKCDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAERQIGILFQDALLFDQFSVGQNLLLALP
                     AALKGVARRDAVNDALERAGLGGMFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRAQAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            13558..14865
                     /locus_tag="IU325_01755"
     CDS             13558..14865
                     /locus_tag="IU325_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HAP5807760.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQILNGPSGHEPAALNLSASWLDKMSTEQLNAWIKLHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(14874..15494)
                     /locus_tag="IU325_01760"
     CDS             complement(14874..15494)
                     /locus_tag="IU325_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP5807761.1"
                     /translation="MLDRHLHPRIKPLLHQCVKVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFVWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            15581..15988
                     /locus_tag="IU325_01765"
     CDS             15581..15988
                     /locus_tag="IU325_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine (deoxy)nucleoside triphosphate
                     diphosphatase"
                     /protein_id="HAP5807762.1"
                     /translation="MKMVEVVAAIIERDGKILLAQRPAHSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEAAVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(15954..16226)
                     /locus_tag="IU325_01770"
     CDS             complement(15954..16226)
                     /locus_tag="IU325_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1496 domain-containing protein"
                     /protein_id="HAP5807763.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            16462..17805
                     /locus_tag="IU325_01775"
     CDS             16462..17805
                     /locus_tag="IU325_01775"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013366584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP5807764.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI"
     gene            complement(17882..19837)
                     /gene="topB"
                     /locus_tag="IU325_01780"
     CDS             complement(17882..19837)
                     /gene="topB"
                     /locus_tag="IU325_01780"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP5807765.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTSVRQFRGIMAPGGGE
                     GKKKDSPRKRAPKKSPPSEEAGNGVIT"
     gene            complement(19842..20885)
                     /gene="selD"
                     /locus_tag="IU325_01785"
     CDS             complement(19842..20885)
                     /gene="selD"
                     /locus_tag="IU325_01785"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HAP5807766.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(21002..21553)
                     /locus_tag="IU325_01790"
     CDS             complement(21002..21553)
                     /locus_tag="IU325_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HAP5807767.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAGGSDDKAIDKARNAPYRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            21714..23570
                     /gene="sppA"
                     /locus_tag="IU325_01795"
     CDS             21714..23570
                     /gene="sppA"
                     /locus_tag="IU325_01795"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP5807768.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGVLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKTLTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            23734..24750
                     /gene="ansA"
                     /locus_tag="IU325_01800"
     CDS             23734..24750
                     /gene="ansA"
                     /locus_tag="IU325_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP5807769.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            24761..25402
                     /gene="pncA"
                     /locus_tag="IU325_01805"
     CDS             24761..25402
                     /gene="pncA"
                     /locus_tag="IU325_01805"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HAP5807770.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTTLDDWLRDHEIDELIIMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQE"
     gene            complement(25495..26853)
                     /locus_tag="IU325_01810"
     CDS             complement(25495..26853)
                     /locus_tag="IU325_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5807771.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIVILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSVRLPPLTLNQSNSKANVINGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(26971..27729)
                     /locus_tag="IU325_01815"
     CDS             complement(26971..27729)
                     /locus_tag="IU325_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP5807772.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            complement(27866..28846)
                     /gene="ydjG"
                     /locus_tag="IU325_01820"
     CDS             complement(27866..28846)
                     /gene="ydjG"
                     /locus_tag="IU325_01820"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HAP5807773.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIRKEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKDE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMESELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            complement(28856..29803)
                     /locus_tag="IU325_01825"
     CDS             complement(28856..29803)
                     /locus_tag="IU325_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP5807774.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNCNGSLWKLNIDDVDFSRFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNYAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGASTGVKNRKLVEQLLEECEG"
     gene            complement(29808..30644)
                     /locus_tag="IU325_01830"
     CDS             complement(29808..30644)
                     /locus_tag="IU325_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP5807775.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAKKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDTDIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            complement(30665..31708)
                     /locus_tag="IU325_01835"
     CDS             complement(30665..31708)
                     /locus_tag="IU325_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP5807776.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKAGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDMMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATDIAVVDVLDKRLTMAEQLGATVVI
                     NGAKEDTIARCQQFTEEMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYQDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            complement(31725..33104)
                     /locus_tag="IU325_01840"
     CDS             complement(31725..33104)
                     /locus_tag="IU325_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5807777.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVSFGLLVCWSNAVGGL
                     ILAQLKELGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALMTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVVNK"
     gene            complement(33131..34207)
                     /locus_tag="IU325_01845"
     CDS             complement(33131..34207)
                     /locus_tag="IU325_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAP5807778.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAILDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNALRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            complement(34577..34849)
                     /locus_tag="IU325_01850"
     CDS             complement(34577..34849)
                     /locus_tag="IU325_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1315 family protein"
                     /protein_id="HAP5807779.1"
                     /translation="MNLDDIINSMTLEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKAIAMFK"
     gene            complement(34891..35304)
                     /gene="msrB"
                     /locus_tag="IU325_01855"
     CDS             complement(34891..35304)
                     /gene="msrB"
                     /locus_tag="IU325_01855"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP5807780.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            35646..36641
                     /gene="gapA"
                     /locus_tag="IU325_01860"
     CDS             35646..36641
                     /gene="gapA"
                     /locus_tag="IU325_01860"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP5807781.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            36725..37609
                     /locus_tag="IU325_01865"
     CDS             36725..37609
                     /locus_tag="IU325_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HAP5807782.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAYFRVGKTCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(37660..38514)
                     /gene="yeaE"
                     /locus_tag="IU325_01870"
     CDS             complement(37660..38514)
                     /gene="yeaE"
                     /locus_tag="IU325_01870"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HAP5807783.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFALEETVAAMDKLVAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQIPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSPAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(38604..39350)
                     /locus_tag="IU325_01875"
     CDS             complement(38604..39350)
                     /locus_tag="IU325_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltA-interacting protein"
                     /protein_id="HAP5807784.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            39786..41720
                     /locus_tag="IU325_01880"
     CDS             39786..41720
                     /locus_tag="IU325_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HAP5807785.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            41833..43116
                     /locus_tag="IU325_01885"
     CDS             41833..43116
                     /locus_tag="IU325_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HAP5807786.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            43263..44738
                     /locus_tag="IU325_01890"
     CDS             43263..44738
                     /locus_tag="IU325_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAP5807787.1"
                     /translation="MIRSTRVSLGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MASMGTQKLKAQGFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVIDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            44919..46409
                     /locus_tag="IU325_01895"
     CDS             44919..46409
                     /locus_tag="IU325_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAP5807788.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     RADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRTICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            46452..46955
                     /gene="yeaK"
                     /locus_tag="IU325_01900"
     CDS             46452..46955
                     /gene="yeaK"
                     /locus_tag="IU325_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HAP5807789.1"
                     /translation="MTEMAKGSMTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(46956..47060)
                     /locus_tag="IU325_01905"
     CDS             complement(46956..47060)
                     /locus_tag="IU325_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5807790.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            47230..47676
                     /locus_tag="IU325_01910"
     CDS             47230..47676
                     /locus_tag="IU325_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 family protein"
                     /protein_id="HAP5807791.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(47633..48454)
                     /locus_tag="IU325_01915"
     CDS             complement(47633..48454)
                     /locus_tag="IU325_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP5807792.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTSTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGSLGQWARFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            48551..49732
                     /gene="nimT"
                     /locus_tag="IU325_01920"
     CDS             48551..49732
                     /gene="nimT"
                     /locus_tag="IU325_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HAP5807793.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     FLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMSFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGVAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDTNGDWSIPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            49787..50134
                     /locus_tag="IU325_01925"
     CDS             49787..50134
                     /locus_tag="IU325_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP5807794.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(50156..50410)
                     /gene="yoaF"
                     /locus_tag="IU325_01930"
     CDS             complement(50156..50410)
                     /gene="yoaF"
                     /locus_tag="IU325_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HAP5807795.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            50593..51617
                     /gene="dgcP"
                     /locus_tag="IU325_01935"
                     /pseudo
     CDS             50593..51617
                     /gene="dgcP"
                     /locus_tag="IU325_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
     gene            51650..51748
                     /gene="yoaK"
                     /locus_tag="IU325_01940"
     CDS             51650..51748
                     /gene="yoaK"
                     /locus_tag="IU325_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HAP5807796.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            51751..51825
                     /gene="yoaJ"
                     /locus_tag="IU325_01945"
     CDS             51751..51825
                     /gene="yoaJ"
                     /locus_tag="IU325_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HAP5807797.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(51884..52132)
                     /locus_tag="IU325_01950"
     CDS             complement(51884..52132)
                     /locus_tag="IU325_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP5807798.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(52280..52462)
                     /locus_tag="IU325_01955"
     CDS             complement(52280..52462)
                     /locus_tag="IU325_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HAP5807799.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(52466..52825)
                     /gene="yeaR"
                     /locus_tag="IU325_01960"
     CDS             complement(52466..52825)
                     /gene="yeaR"
                     /locus_tag="IU325_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HAP5807800.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYPDEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(52998..53636)
                     /gene="leuE"
                     /locus_tag="IU325_01965"
     CDS             complement(52998..53636)
                     /gene="leuE"
                     /locus_tag="IU325_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HAP5807801.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(53763..54686)
                     /locus_tag="IU325_01970"
     CDS             complement(53763..54686)
                     /locus_tag="IU325_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP5807802.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQLLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            54789..55874
                     /gene="dmlA"
                     /locus_tag="IU325_01975"
     CDS             54789..55874
                     /gene="dmlA"
                     /locus_tag="IU325_01975"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HAP5807803.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEICW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            56125..57735
                     /gene="yeaV"
                     /locus_tag="IU325_01980"
     CDS             56125..57735
                     /gene="yeaV"
                     /locus_tag="IU325_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HAP5807804.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLKEGDDPDRALRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEAPEKTPVLPAGTVFKGDN"
     gene            57767..58891
                     /gene="yeaW"
                     /locus_tag="IU325_01985"
     CDS             57767..58891
                     /gene="yeaW"
                     /locus_tag="IU325_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HAP5807805.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            58947..59912
                     /gene="yeaX"
                     /locus_tag="IU325_01990"
     CDS             58947..59912
                     /gene="yeaX"
                     /locus_tag="IU325_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HAP5807806.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFMLVATDGKPLPAFTGGSHVI
                     VQMSDGDNEYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHLAELQYSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHFHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAAHLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(59966..61081)
                     /locus_tag="IU325_01995"
     CDS             complement(59966..61081)
                     /locus_tag="IU325_01995"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HAP5807807.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPISWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(61163..62848)
                     /gene="fadD"
                     /locus_tag="IU325_02000"
     CDS             complement(61163..62848)
                     /gene="fadD"
                     /locus_tag="IU325_02000"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HAP5807808.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(63053..63634)
                     /gene="yeaY"
                     /locus_tag="IU325_02005"
     CDS             complement(63053..63634)
                     /gene="yeaY"
                     /locus_tag="IU325_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HAP5807809.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(63674..64369)
                     /gene="tsaB"
                     /locus_tag="IU325_02010"
     CDS             complement(63674..64369)
                     /gene="tsaB"
                     /locus_tag="IU325_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP5807810.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAGDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(64427..66337)
                     /locus_tag="IU325_02015"
     CDS             complement(64427..66337)
                     /locus_tag="IU325_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP5807811.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            66469..66813
                     /locus_tag="IU325_02020"
     CDS             66469..66813
                     /locus_tag="IU325_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAP5807812.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT        16480 a        17411 c        16321 g        16674 t
ORIGIN      
        1 aagaaagtaa cttaataccc gcagaatgat ttctgcgggt aagtattagc ttattttttc
       61 gagcattaat cccgcgcgta atcccaacgc taccaaagga ttagggaata acacatactc
      121 tacatcatgg gttacggtaa aacgttcctc cccgtcctgc gccagcaatg ttcctttctc
      181 aaacggcata aaattcagcg tgtcacttgc catatgcatt tcgaaggacg gcgagtggcg
      241 agtaatttgc gaaaccaccc gataacggag cggcggcgtt ctcacgatac caacactctc
      301 accagatagc agcgcagcaa tggcgctggc agtgactgca aactggcgaa gatcgttttg
      361 tccaaagggc aacgctttgc cgagttccag cgtacaggcc agcgcgccaa aatgtctggc
      421 gctgaaatga gtaaacgtac caccaggcgt ctgatggaat accagtgcct ccagccccgc
      481 cgcccccagc cacgtcagaa atttctcgtc ccagggaatg tcgcgttgcg gtaatacacc
      541 gaactgcgga tgcaaggagc cacgaattgc ggtatggaga tcaaggtgcc agcgcacaga
      601 ttctttgccc tggtcataaa aatcttccag gcactgttcc agttcgcgcg cgcgacaggt
      661 ttccccgctt tcagcaaata gctgccaacg accgccaaac attcggttca tatcgctatg
      721 gcaataacgt ttcccttgct tcagcgcagg aggattcccg aggatcacca gcaaccgcca
      781 acgtaacggg atctcgccgt gagatatcgc gccaagcaac gcgtccagca tctccacagg
      841 tgccgtctca ttaccgtgta ttcccgctga aatcaccagt gcgccttgcg gtggcgttaa
      901 tggcgtcagt tccagcacac catcgcccag ccagcgccag cgaacgccgt tgatttctcg
      961 ctcggtgata accggttttt tacccgttaa ggtcagagca agaaaattat ccattgcccc
     1021 ctccctcgcg ctggaacgga taaaccgaac cgagattcag taattggctc aatacatcca
     1081 gtgcttcccg cccttcgcgc agcaattgcg ggtcggccag atcggcagca gtaagacgat
     1141 cgcggtagta acgatccacc cagtcattga gcgcattaaa cagcgtatcg ttcatcatca
     1201 ccgccggatt caccgcccgg cgttcttctt ctgtcaatac cacccgcaac cgcaggcacg
     1261 ccgggccgcc accattcgcc atgctttcac gtaaatcaaa gacttttagt tcgctaatcg
     1321 ggttgtcagc ggcaaggagt tcattgagat aaccccatac tccggcgtgt tcccgacact
     1381 cctgaggcag caccaacatc atggaaccat catcgcggct caacagttgg ctgttaaaca
     1441 gatatgtaga caccgcatcc gacacggaaa cctgagtcgc cggaacttct atcgccataa
     1501 aaccgttcac ccgcgcacgc aggtttgcca gtaactgcac ctgtcgagcg aacgcctgtt
     1561 gatgacaaaa cagcacctgg cggttactca cggcaatcac gtcattatga aaaacgccct
     1621 ggtcgataac gtccgggttt tgctgggcga aaatcacctg ttggggattc acctgattca
     1681 accttgccac cgcctcgctg gcttcgcgag tctgtcgcgc cggataacgg gaaggccggg
     1741 tatcattgcc ctcttctcgc ccgtagacaa aaagttgcat acccggttca ccgtaatgac
     1801 cgccgagacg attgtggttt gccgccccct catcaccgag caaggctacc tgcggcaacg
     1861 ccgaatggac actaaatttc tcttcgtcgt taaaaatcgc ttttaacagc gattcagaga
     1921 cgggcgcttc cagcgaacgg tgaaatttat tgttcaggtt agcaacggtg agatgcactt
     1981 tgccatccag cgtatcggtg gatggcgcta ccgtggccgc attagctacc cacattggcg
     2041 aagcggaact gacgctggaa agccagtgcg gtgcctggcg ggcaaccttt tccagtacct
     2101 gctcatcgct gccgctgaaa cccaactgac gcagcaccgg aataaacgga cgctcgtgcg
     2161 gcgggatcac cgcctggggg aatcccgcgt cggcaagggc tttcattttc agtaagccct
     2221 gcttcgccgc cagtcgcggg ttagagacct gaaaacggtg actggtggag gcttcattac
     2281 caaacgacag gcccgcgtaa tgatgcgtca gccctaccag cccgtcgaaa ttgatttccc
     2341 aggcgttcat cgcaccacct catcggaaaa atccagccca gggttaagcg tggcgggcaa
     2401 tgttaacgag tccgactcca gactcgccat cggccaggcg cagtaatctg cggcatacca
     2461 ggcgctgggg cgatggttac cggaagcgcc aatgccgccg aatggcgcgg tactggcagc
     2521 gccggtaagc ggtttgttcc agttaacaat ccccgcccgc gcctccagca acagttgatc
     2581 gaatttttcc cgcgcggggg aaaccagtcc gcaagagagt ccgaagcgag tgttattcgc
     2641 cattcgaatc gcttcatcga aagtatcata acgccagacg cgcaataacg gtccgaacac
     2701 ctcttcatct ggtacgccag caacgcctgt catttcaatg atccccggcg tcagcaacga
     2761 tgtccctgct tgtaataagc gcggcgcaag cagggttcgt ctgcccatcg cttccagttg
     2821 ctgccaggca gtaaccacct gctgtgcggc ctgttcagaa atcagcccgc caataaacgg
     2881 ctgcggttcg tcatcccatt tgcccggcgt taatcgctgg ctgacggcaa ccagacgagc
     2941 aagaaacgca tcgccctgcg ccccgctttt cagcaataaa cggcgggcgc aggtgcagcg
     3001 ttgcccggct gtgacaaatg ccgactgaat ggtcagatgg acagcggcat cgatatccgc
     3061 cacctcatcg ataatcagcg ggttattgcc gcccatttcg agggcgagaa ttttctccgg
     3121 ctgaccggaa agctgacgat gcaactggta gccagtattg gcgctaccgg taaacagcaa
     3181 gccgtcgaga tcctccagcg cactcagcgc ctgacccgtt tcacgcccgc cctgcaccag
     3241 gttcagcacg cccggcggca agccagcctg ctgccataaa cgcattaccg cttcgccgct
     3301 ccagggtgtc agttcgctgg gtttaaagat aatggtgtta cccgccagca atgccggaac
     3361 gatatgcccg ttcggcaaat gaccagggaa attatacggc ccaaacaccg ccagcacgcc
     3421 gtgcgggcga tgtcgcaggc tcgccgcgcc gtccggcatt tcgctacgct gctcgccggt
     3481 acgaacatga tacgccttaa ttgatatcgc gattttattg atcatcgccg tcacttcggt
     3541 tgccgcttcc cagcgcggct tacccgtttc tctggcaata atcgcggtta attcggcttt
     3601 attgctttcc agcaatccgg caaagcgttc aactcttacc tgacgatcgc caaaggagag
     3661 acgcgcccag cgcggaaacg ccgcacgggc tgcccgacaa gcctgcccga cctgagcggc
     3721 atcggcatca ttgccttgcc ataacacctc gcccgatacc ggattacgct tcacacgcaa
     3781 tgcgccctgg cccgttaccc aatcaccgtt aatccataaa gtcatgctgt tttctcctct
     3841 gcgcacaggc gcaccagacg aacgcgatcc cctgcgtggc atttgagggc atccagttgt
     3901 gcggcggtta aaatcaaacg ctcggttgcc ggatcggtac gcaccagcac cacgcggaaa
     3961 tggtgataat tttcattggc gaccaagcag gccggaaaat cgccctgcgc aggctgccct
     4021 tctgccactt ccaccagccg acttttacgg atggcgcgca cgcggtcgat gtcacactca
     4081 agcgtcggcc caccgtcgaa gatgtcgata taattacggt agcgaaaacc ttctttctcc
     4141 agtaccgcac gggcaggcgc ggtttgcgga tgtacctgac cgatgacgtc ctgggcttcc
     4201 tgagataaaa agtgggtata gatcggatgt ttcggcatca gttctgcaat aaatgccttt
     4261 tgcccggtgc cgcagagaaa atcggcgcgg ctaaaatcca tcgaaaagaa gcgcttaccg
     4321 aggctttgcc agaacggtga atagccatgt tcgtcaatca ccccgcgcat ttcggcaacc
     4381 actttgtcat taaacttgtc gcgaaaagcc gccataaaca taaagcgcga tttcgacagc
     4441 aaatagccgt tgccctcttt gcgccactcc gggtcgagaa acagcgtgca cagctcgctg
     4501 ctgccggtgt gatcgttact gagaaacagc gtcggcaacg cgttatagac attcagctct
     4561 ttcgaagcgt gaaccagcgt gccgacgcga tagttgtacc agggatcgtt cagcccaact
     4621 gccacctcaa tggcgcaaat ccccgccact gtgcctgtct cgctatcttc cagcacgaac
     4681 acatagccct gctcactttt gggcagttcg ccttgccagg ttttgattgc cctttcgata
     4741 cgcgccgaaa gcgtggcttc attggcggga agcgacgtca ggccgccgcc agttttgctg
     4801 gcaagctgca tcagcgccga gatatctgaa cgctcaacgg gacggatgac catcatgaag
     4861 aacctcggct aacaaagtgt tcgcaagcag ctgcaaagcg atccagtccg gtcgtcacct
     4921 cttcttcgct aacattgagc gcaggcgcaa aacgcaccac gttgccaccc gcaatcagta
     4981 ccatcacgcc tgctttcgcc gcttcctgag agatctgttt cgcttgcccg gcgtaatcgg
     5041 cattcaacac gcaaccaatc aacaaaccta agccgcgaac ttcactgaac aaaccgtagc
     5101 ggtgattaat ggtattaaga cgctcaacaa accagtcgtg acgctgttta acgccattaa
     5161 gcatctctgg cgtgttgatg agatccagca ctttgcctgc caccgccgag gccagcggat
     5221 tgccgccata ggtggtgcca tgagtgccaa cggtcatcac actggcgcac tcttcggttg
     5281 ccaacaacgc accaaccggg aaaccgccgc ccagcgcttt ggcggtagtt aacagatcgg
     5341 gcgtcacgcc gtagtgcata taggcataca gttccccggt gcgtccgacg ccggtttgta
     5401 cttcatcaaa aatcagcaat gcattgtggc ggtcacacaa ttcacgcaga ccatgtaaaa
     5461 acgcgttgct ggctggcacc acaccgcctt ccccctggat gggttcgaca atcaccgcac
     5521 aggtagagtc gtcaatcagc gcgctggcag agttaatatc gttatatgcg gcatgaagaa
     5581 tatccggcgg cagtggcgca aaatcctgtg aataggctgg ctgcccaccc gcactgacag
     5641 taaacagcgt gcgaccatga aacgcatttt taaacgccac gataccgctc ttatggctgc
     5701 cgtagcggtc gtgagcgaat ttacgcgcca gttttagcgc cgcttcattg gcttccgcac
     5761 cggagttaca aaagaagacg cgatcggcaa acgtggcgtc ggtcaatttt ttcgccagtc
     5821 gcagtaccgg ctcgttggtg taaccgttgc cggtatgcca gaacttactc gcctgttcgt
     5881 tcagcgcctc acgcagttcc gggtgcgcat ggcccagcgc gttcaccgca atgccacccg
     5941 cgaagtcgat atactctttc ccctgctgat cccacaagcg cgaaccttcg ccacgtaccg
     6001 gtataaacgg tgccggagcg taaacaggta tcatccattc atcaaagttt tcacgcgtaa
     6061 ttggctgaga catagcgacc tctacagtaa ataattcgtt atttatatgt taataataag
     6121 taatgtttgc gctgtaaatg tagattgcag ggttcgtgcc agccagtgat aaaagtgcat
     6181 aaatcgcgga ggctaactgg aaatcaagga gttataacca aaccatatgc atttaaagtg
     6241 catataaagt gaatacgttt gcgatgtggg tgaataaaaa gaataaaaaa cgcaatgtta
     6301 tgcagaagta aaatataatt ctggaattgt gatcattgac gaaatttact ggaaattact
     6361 gcgccattct gacgcagcgc gcaccaaaag cgggcatttt ttgcgccatc gttgacatca
     6421 ttaacaacta tcgatcaaat cacttaacaa caggcggtac gcaaagcgaa attctgctac
     6481 catccacgca ctctttatct gaataaatgg cagcgactat gaaatttgtc tcttttaata
     6541 tcaacggcct gcgcgccaga cctcatcagc ttgaagccat cgtcgaaaag caccaaccgg
     6601 atgtgattgg cctgcaggag acaaaagttc atgacgatat gtttccactc gaagaggtgg
     6661 cgaagctcgg ctacaacgtg ttttatcacg ggcagaaagg ccattatggc gtggcgctgc
     6721 tgaccaaaga gacgccgatt gccgtgcgtc gcggttttcc gggtgatgac gaagaggcgc
     6781 aacggcgaat tattatggct gaaatcccct cgacgctggg caatgttacc gtgatcaacg
     6841 gttacttccc gcagggtgaa agccgcgacc atccgataaa attcccggca aaggcgcagt
     6901 tttatcagaa tctgcaaaac tacctggaaa ccgaactcaa gcgtgaaaat ccggtgctga
     6961 ttatgggtga tatgaatatc agtcctggcg acctggatat tggcattggc gaagagaacc
     7021 gtaagcgctg gctgcgtacc ggaaaatgct ctttccttcc ggaagaacgc gaatggatgg
     7081 agagactgat gagctggggg ttggtcgata ccttccgcca tgcgaatccg caaacagccg
     7141 atcgtttctc atggtttgat taccgctcaa aaggttttga cgataaccgt ggtctgcgca
     7201 tcgacctgct tctcgccagc caaccgctgg cagaatgttg tgttgaaacc ggcatcgact
     7261 atgaaatccg cagcatggaa aaaccgtcag atcatgcccc cgtctgggcg accttccgcc
     7321 gctaatttag ctgttctcct ggctcaaact gggtcaggag aattaacctt gagaaaaatc
     7381 aacaaactgt cagtaatgat ttgttgcctg ccgccctttg ttataccgtc actgcgtttt
     7441 tagttgtctg accacttctc tattatcaag tttgatatag gaaactccac aatgaacgct
     7501 gagcgtaaat ttctttttgc ctgtcttatt tttgcgctgc tcatttacgc tatccacgct
     7561 ttcggtttat tcgaactgct caccgattta ccccacttac agacactcat tcgccagagc
     7621 ggattgttcg gttatagcct ctatattctg ttattcatca ttgccaccct ctttctgtta
     7681 ccaggaagca tactggtgat cgctggagga atagtttttg gcccgctctt agggacacta
     7741 ctatcattaa ttgccgccac gctggcctcc tcgtgctcat tcctgctggc gcgctggctg
     7801 ggacgtgatt tactgctgaa atacgttggc catagccata ccttccaggc cattgaaaaa
     7861 ggcattgcgc gtaacggtat tgattttctt attctgaccc gcttaatccc gttgtttcct
     7921 tacaatattc aaaattacgc ttacggatta accaccatcg ccttctggcc ttataccctt
     7981 atttcggcac tcacgaccct gcccggtatt gttatttata ccgtgatggc aagcgatctc
     8041 gccaatgaag gcattacgct gcgctttatt ttacaactct gtctggcggg cctggcgctg
     8101 tttattctcg tccagctcgc caaactctac gcccgacaca aacatgtgga tctgtctgct
     8161 tcgcgccgca gcccacttac tcacccaaaa aatgaaggat agaacgatgt tgcaacatta
     8221 ttcagcgtca tggaaaaaag gactggctgc actctgctta ctggctgttg ccgggcttag
     8281 cggctgcgat caaaaagaga atgcggctgc aaaagtggaa tacgacggac tttcgaacag
     8341 ccaaccgctg cgtgtcgatg ccaataacca tacggtaacc atgctggtgc aaattaatgg
     8401 tcgtttcctg accgacgaca cccgccacgg cattgtgttt aaagatggct caaacgggca
     8461 taaatcgctg tttatgggtt atgcgacccc gaaagcattt tatgaagccc tgaaagaggc
     8521 aggtggtaca ccgggcgaaa acatgacgat ggataataaa gaaacgactc acgtcgcagg
     8581 cagcaaactg gatatttcgg tcaactggca aggggcggca aaagcgtatt ccttcgatga
     8641 agtgattgtt gacagtaatg gcaagaaact ggacatgcgc tttggcggta atttaacggc
     8701 agcagaagag aagaaaacgg gctgcctggt gtgtctggat agctgcccgg tcggcatcgt
     8761 cagcaatgca acatacactt atggtgcggt ggaaaaacgc ggcgaagtga aattcaaagg
     8821 caatgcctcg gttctcccgg cggataacac gctggcaacg gttaccttta aaatcaccga
     8881 ataaagccag gataaaggat gatgaagatg caatcgcgaa aaatctggta ctaccgtata
     8941 accctcatca tcctgttgtt cgctatgctg ctggcatggg cgctgcttcc cggcgtccat
     9001 gagtttatta atcgcagcgt tgcggcgttt gccgccgtgg accaacaggg tatagaacgc
     9061 tttattcagt cttacggcgc actggcggca gttgtctcgt tcttgttgat gattttacag
     9121 gccattgctg caccgctacc tgcgtttttg atcacctttg ccaatgcgtc gctgtttggc
     9181 gcgctctggg gcggcttgct gtcgtggacc agttcgatgg ccggcgcggc gctgtgcttt
     9241 tttatcgcca gagtgatggg ccgcgaagtg gtggaaaagt taacgggtaa aacggtactc
     9301 aatagtatgg acggcttttt tacccgctat ggtaaacaca ccattctggt atgccgatta
     9361 ttgccttttg tgccttttga tcccgtaagc tatgctgccg gtttgacttc aatacgtttt
     9421 cgctcgtttt ttatcgccac cgggcttggt cagttaccag cgactattgt ttattcctgg
     9481 gcgggcagca tattaacagg cggtactttc tggtttgtca ccggactgtt tattctgttt
     9541 gccctgaccg tggtgatttt tatggcgaag aaaatatggc ttgagcgcca gaagaggaat
     9601 gcctgatggg tttaccgccg cttagcaaaa ttcctttcat tttacgtccg caggcgtggc
     9661 tgcatcgtcg ccattacggc gaggtgctaa gcccgattcg ctggtggggg cggatcccgt
     9721 ttatctttta tctggtgtcg atgtttgtcg gctggctgga gcgcaaacgc tcaccgctgg
     9781 atccagtagt gcgatcgctt gtcagcgcgc gcattgcgca aatgtgtctg tgtgagtttt
     9841 gcgtggatat caccagtatg aaagtcgccg agcgcactgg cagcaccgat aaactgctgg
     9901 cagtggctga ctggcggcaa agcccgctct ttagcgatga agaacggctg gcgctggagt
     9961 acgccgaagc cgcaagcgta acgccgccaa cggtcgatga tgccctgcgt acccgactgg
    10021 ctgcgcattt tgacgcccag gcgctcaccg aactgacggc attgatcggc ctgcaaaacc
    10081 tgtcagcccg ttttaattct gccatggaca ttcccgctca ggggctgtgc cgtattcctg
    10141 aaaaacgttc ttaaggagag atgatgcgcc attgtgggtg gttgctggga ttgttatcgc
    10201 tgttttcttt ggcaacacat gccagtgact ggcaggaaat aaaaaatgag gccaaaggac
    10261 aaaccgtctg gtttaacgcc tggggcggcg ataccgcaat taaccgctat ctcgactggg
    10321 ttagcggcga gatgaaaacc cattacgcta taaacctgaa gattgttcgt ctcgcggatg
    10381 ccgcagacgc ggtgaagcgc attcagaccg aagctgctgc cggacgcaaa acaggcggct
    10441 cggtggatct gctctgggtg aacggcgaaa acttccgcac cttaaaagag gccaatttat
    10501 tacaaacggg ctgggcggag actctgccca actggcgcta tgtcgacaca cagctgccgg
    10561 tgcgggaaga tttttcagtg cctacagaag gggctgaatc gccctggggg ggcgcgcaac
    10621 tgacgtttat cgcccgccgc gatgttacgc cgcagccacc acaaacgccg caagccttac
    10681 tggagtttgc taaagccaat cccggtacgg ttacctatcc gcgcccaccg gactttaccg
    10741 gcacggcgtt tcttgaacag ttgctgatta tgctaacgcc agatcccaca gcattaaaag
    10801 aagcgccgga cgatgcgact ttcgcccgtg tcactgctcc cttgtggcaa tatcttgatg
    10861 cgctgcatcc gtatttgtgg cgcgaaggaa aggatttccc gccttcgccc gcgcggatgg
    10921 atgctctgct gaaagccggc acattgcgcc tgtcgctgac ctttaatccc gcgcatgcgc
    10981 agcaaaaaat cgccagcggc gatttacccg ccagcagtta cagctttggc tttagcgagg
    11041 gaatgattgg caacgtgcat ttcgtcacca ttcccgccaa cgcgaatgcc agtgctgcgg
    11101 cgaaggtagt tgccaatttc ctgctctcac ccgatgcaca actgcgtaaa gcagaccctg
    11161 ctgtctgggg cgatccttcg gttctcgatc cgcaaaaact gcctgacggg cagcgcgaaa
    11221 tattgcaatc aagaatgccg caggatctgc cgccggtact ggctgaaccg cacgcaggtt
    11281 gggtaaatgc gctggaacaa gaatggctac accgttacgg tacgcattaa tctttttgct
    11341 gtgggcgatg gtggcggtga tttatgcacc gctgatcccg gcagctctca cgctgatatc
    11401 gcctgctttg tcgttgacac actggcagac gttatttgcc gatccacagt taccgcaggc
    11461 attactggca acgctggtgt cgacaaccat cgcggcggtc ggggcgttgt tgattgccct
    11521 gctggtgatt gtggcgctgt ggcctgggcc gaaatggcag cgtatgtgcg cccgtctgcc
    11581 gtggctgctc gccattcccc atgtggcttt tgccaccagc gcccttctgc tctttgctga
    11641 cggagggctg ctttatgact atttcccgta tttcactccg ccaatggacc gatttggcat
    11701 cggtctgggc ctcacccttg cagtgaaaga aagcgcattt ctgctgtgga tcttagcggc
    11761 agtattgagc gaaaaacggc tgttgcagca agttattgta ctggattcgc tgggctacag
    11821 ccgctggcaa tgcctgaact ggctgttgtt gccctccgtc gcacctgcac tggcaatggc
    11881 gatgctggcg attgtcgcct ggtcgctgtc ggtcgtggat gtggcgatta tccttgggcc
    11941 aggtaatccc ccgacgctgg cagtaattag ctggcagtgg ttaactcagg gcgacgccga
    12001 tcaacaaacg aaaggcgcac tcgccagcct gctgctgatg ctgttactcg ccgcctacgt
    12061 tttgctgggc tatctgctat ggcgtagctg gcggcgtact attccccgcg tagatggcgt
    12121 tcgtaagcct gccacgcctt tattgccagg caatacgctg gcgagttttt tacccttaac
    12181 cggtgtgctg tgtgtggttc tgctggcgat cctcgcggat cagtcgacga tcaatagtga
    12241 agcgctcatc aacagcctga caatggggct ggtggcggca ttcatcgctt tgctcctgtt
    12301 actgctgtgg ctggaatggg ggccacagcg tcgccagttg tggctatggt tgcccatttt
    12361 attacccgct ctgccgctgg tggcgggcca gtacacgctg gcgctatggc tgaacctgga
    12421 tggaagctgg acggcggtgg tctgggggca tctgctgtgg gtaatgccgt ggatgctgtt
    12481 tatcctgcaa cccgcctggc agcgcattga ttcacggtta attttgattg cgcaaacact
    12541 gggctggtcg cgggccaaaa tcttctttta cgtgaaatgc ccactcatgc tacgccctgc
    12601 gctgattgtc ttcgcggtgg gattttcagt cagtattgca cagtatatgc cgacgctgtg
    12661 gctgggcgcg gggcgttttc cgacgctcac cactgaagcg gtggcattaa gcagcggcgg
    12721 cagcaacggt attctcgccg cccaggcttt atggcaacta ctgttgccgc ttattatttt
    12781 tgccctgacc gcgttagtcg caaaatgggt aggttatgtc agacaaggac tccgctaatg
    12841 ctctgcgtga aaaatgtttc gctacgttta ccagaaagct gcttgctgac aaacgttaac
    12901 tttacggtgg ataaatgtga cattgtcacg ttaatggggc cgtctggctg tggaaaatcc
    12961 actctgtttt catggatgat tggtgcgctg gccggacagt tttcttgtac aggtgagcta
    13021 tggctcaatg agcaacggat tgacatgcta cccaccgcag agcgtcagat tggcattctt
    13081 ttccaggatg cactgttatt tgaccagttc agcgtcgggc aaaatttact gctggctctg
    13141 ccagcggcac tcaaaggtgt tgcaaggcgc gatgcggtaa atgatgcgct ggagcgtgcg
    13201 gggcttggcg gtatgttcca tcaggatcct gccactttgt ctggcggtca gcgagcgcgc
    13261 gttgctctgc ttcgcgctct tctcgctcag ccaaaagcgt tgctactgga tgagccattc
    13321 agccgtcttg atgtagctct gcgcgataat tttcgccagt gggtgttcag cgaagttcgc
    13381 gcccaggcga tcccagtcgt tcaggtaacg cacgatctcc aggatgttcc tgctgatagt
    13441 tctgttctgg atatggcgca gtggtcagaa aattacaaca aactgcgata acgcaaagtt
    13501 tttcccaatg cgtcagttca gaatggcgca ctcaaaacta caatgtcggg attttcgatg
    13561 aaacgtgttt ctcaaatgac cgcgctggca atggctttag ggctggcttg cgcttcttcg
    13621 tgggccgctg aactggcgaa gcctcttaca cttgaccagc ttcaacaaca aaatggcaaa
    13681 gcgatagata ctcgccccag cgcgttttat aacggctggc cacaaatctt aaatggccct
    13741 tctggtcatg agcctgccgc cttaaacctc tctgccagct ggctcgacaa aatgagcacc
    13801 gaacagctta acgcgtggat caagctacat aacctgaaag ccgatgctcc ggttgcgctg
    13861 tacggtaatg acaaagatgt cgatgccgtc aaaacgcgtc tgcaaaaagc aggttttacg
    13921 catatctcca tcctgagtga cgcgctaagt gaaccttccc gtctgcaaaa actgccgcac
    13981 tttgagcagc tggtttatcc gcaatggctg cacgacctgc aacaaggtaa agaggttacg
    14041 gcgaaacctg ccggtgactg gaaagtcatt gaagcggcct ggggcgctcc taagctttac
    14101 cttatcagtc atattcccgg cgctgactac atcgatacca acgaagtgga aagcgaaccg
    14161 ctgtggaaca aagtttctga tgaacaacta aaagcgatgc tggcaaaaca cggcattcgc
    14221 catgacacca cggtcattct gtacgggcgt gacgtatacg ctgcagcgcg tgtggcgcag
    14281 ataatgcttt atgccggcgt gaaagatgtc cgcctgctgg atggcggctg gcaaacctgg
    14341 tccgacgcgg gactgcctgt tgagcgcgga acgccaccga aagtgaaagc ggaaccggat
    14401 ttcggcgtga agatcccggc acaaccgcaa ctgatgcttg atatggaaca ggcgcgtgga
    14461 ctgctgcatc gccaggatgc atcgctggtg agcattcgtt cgtggccaga atttatcggt
    14521 acgaccagcg gttacagcta tattaaacca aaaggtgaaa tagccggagc acgttgggga
    14581 cacgctggta gcgactcgac gcatatggaa gatttccata acccggatgg caccatgcgt
    14641 agcgccgatg atattaccgc tatgtggaaa gcatggaata tcaagccaga tcagcaagtt
    14701 tcattctact gcggcaccgg ctggcgcgca tccgaaacct ttatgtacgc acgagcaatg
    14761 ggctggaata acgtctccgt ttacgacggc ggctggtacg aatggagcag cgatccaaaa
    14821 aatccggttg caaccggtga acgcggcccg gacagcagca aataacattg caattactga
    14881 cgctggagcg acttcagcgt cagataccca ctccacaccc gcgtaaaggt cgtcatccag
    14941 cacaacgcac cgaagatcca cgcaaaccac acaaaccatg cagggaataa acatcccagc
    15001 acaaacagta agatcgtttc agtgccttcg gttaatcccc ccagatagta aaacgactta
    15061 tgtgcataac cggggttatc aatctgatgt tttgctgcca gtgcagcaaa ggcgagaaaa
    15121 ctgctgcccg taccgataaa cgcaaacagc agccagccgc ctgccagcgc attttgctcc
    15181 ggtgcagcaa gaataaaacc aaacggcacc agcgcgtaaa agagaaaatc gagagaaata
    15241 tcgagaaagc cgcccgcgtc ggtaagccct ctgcgccgcg ccagcgcacc gtccagacca
    15301 tcaagcagcc tgtttaacaa aatgacgaca agcgccgcca gataccagcc cagcgccaga
    15361 aaaggcagtg ccagcacacc gatggcaaat cccactaatg ttaaaccatc tggcgtgatg
    15421 ccgggtttat ccagcacctt cacacactga tgcaacaaag gtttaattcg gggatgaaga
    15481 tggcggtcta gcacaggcac tccttaaata taaagccttt ctgattgagc aacagtgcgg
    15541 atattatggc atttttcgct tatctacccg tgtgtaattt atgaaaatgg ttgaagttgt
    15601 tgccgccatc atcgaacgtg atggcaaaat tttactcgcg caacgccccg cccatagcga
    15661 tcaggcggga ttatgggagt ttgccggtgg taaagtcgag ccggatgaaa gccagcggca
    15721 ggcgctggtg cgtgagttac gcgaagaact gggcatcgaa gcagctgtgg gtgaatatgt
    15781 tgccagccat cagcgagaag tttcggggcg gattatccat cttcatgcct ggcacgtacc
    15841 cgacttccac gggacgttac aggcacatga acatcaggcg ctggtctggt gctcacctga
    15901 agaggcgctg caatatccgc tggcccctgc tgacattcca ttattagagg cgtttatggc
    15961 tttacgcgcc gccagaccag cggattagtg ctaagggttt tgtcatcacg ctggcattgc
    16021 agcagtattc cttcggcttt aattaccgcc ccttcagaat aattttgatc ctgataaacg
    16081 cagcactgag tacagggctg cgctgactgc ccgcctgaac tgaacacttc tggcggtacg
    16141 tttacctcca cgtccggacg ataatgcggg ttagccagtg caattaatgg aaatgctaat
    16201 actacggcga acaatgctcg actcacaggg aactccttaa cgttattgtc tctgctactg
    16261 ataacggtag ccgggtggca aaactttagc gtctgagtta tcgcatttgg ttatgagatt
    16321 actctcgtta ttaatttgct ttcctgggtc atttttttct tgcttaccgt cacattcttg
    16381 atggtatagt cgaaaactgc aaaagcacat gacataaaca acataagcac aatcgtatta
    16441 atatataagg gttttatatc tatggatcag acatattctc tggagtcatt cctcaaccat
    16501 gtccaaaagc gcgacccgaa tcaaaccgag ttcgcgcaag ccgttcgtga agtaatgacc
    16561 acactctggc cttttcttga acaaaatcca aaatatcgcc agatgtcatt actggagcgt
    16621 ctggttgaac cggagcgcgt gatccagttt cgcgtggtat gggttgatga tcgcaaccag
    16681 gtacaggtca accgtgcatg gcgtgtgcag ttcagctctg ccatcggccc gtacaaaggc
    16741 ggtatgcgct tccatccgtc agttaacctt tccattctca aattcctcgg cttcgaacaa
    16801 accttcaaaa atgccctgac tactctgccg atgggcggtg gtaaaggcgg cagcgatttc
    16861 gatccgaaag gaaaaagcga aggtgaagtg atgcgttttt gccaggcgct gatgactgaa
    16921 ctgtatcgcc acctgggcgc ggataccgac gttccggcag gtgatatcgg ggttggtggt
    16981 cgtgaagtcg gctttatggc ggggatgatg aaaaagctct ccaacaatac cgcctgcgtc
    17041 ttcaccggta agggcctttc atttggcggc agtcttattc gcccggaagc taccggctac
    17101 ggtctggttt atttcacaga agcaatgcta aaacgccacg gtatgggttt tgaaggaatg
    17161 cgcgtttccg tttctggctc cggcaacgtc gcccagtacg ctatcgaaaa agcgatggaa
    17221 tttggtgctc gtgtgatcac tgcgtcagac tccagtggca ctgtagttga tgaaagcgga
    17281 ttcacgaaag agaaactggc gcgtcttatc gaaatcaaag ccagccgcga tggtcgagtg
    17341 gcagattacg ccaaagaatt tggtctggtc tatctcgaag gccaacagcc gtggtctgta
    17401 ccagttgata tcgccctgcc ttgcgccacc cagaacgaac tggatgttga cgccgcgcat
    17461 cagcttatcg ccaatggcgt taaagccgtc gccgaagggg caaatatgcc gaccaccatc
    17521 gaagcgactg aactgttcca gcaggcaggc gtactgtttg caccgggtaa agcggctaat
    17581 gctggcggtg tagcaacatc gggcctggaa atggcgcaaa acgctgcgcg cctgggctgg
    17641 aaagccgaga aagttgacgc acgtttgcat cacatcatgc tggatatcca ccatgcctgt
    17701 gttgagcatg gtggtgaagg tgagcaaacc aactacgtgc agggcgcgaa cattgccggt
    17761 tttgtgaagg ttgcggatgc gatgttaagc cagggcgtga tttaagacaa ttgtgccgga
    17821 tacagcgcaa acgcctaatc cggccataaa aatcttcagt atcagatggt tgaaagaacc
    17881 tttatgttat cactccattg cccgcctctt ctgatggcgg gctttttttc ggcgctctct
    17941 tgcgtggcga atcctttttc ttcccttctc caccgccggg agccattatt ccccgaaact
    18001 gccttaccga cgtgcgcttt gcctgatcaa tcaactggta caatgtcccc accagcggtt
    18061 gcataaagtc ctggtagcga cactgctttt cgctgatttg cgtcagcacc gattcccagt
    18121 gcgcggtcat gtccggtcgc gtcgccatct ctggcagcga atggaataat gcttttccgg
    18181 cgtcggtgga gtggatatag cgcccttttt tggtcaggaa gcctcgcttg aacagcagtt
    18241 caataatccc ggcgcgagtc gcttctgtcc ccagaccatc ggtcgcacga aggatctttt
    18301 tcagatcttt gtcctgcaca aaacgtgcta tcccggtcat cgccgaaagc agtgttgcat
    18361 cggtaaaatg acgcggcggc tgggtttgcc gctctaccac ttcacctttt tcacacagca
    18421 actcatcgcc tttcgccacc acaggcaatg gcgtaccgtc gttttcttca tcgcgctctt
    18481 tgctgcctaa cagcgtgcgc cagcctgctt cagcaagaaa acgcgcttta gcgacaaatt
    18541 tgcctttggc aatatccagt tcgataacac acttgcggaa caccgcatcc gggcagaact
    18601 gcatcaaata ctgacgggca atcaggttat agaccttcgc ttcgttctcc gtcaggttga
    18661 tcgcagaact ccgtgcggtc gggatgatgg cgtggtgcgc atcgaccttt ttgtcatccc
    18721 agcagcggtt gcgtatatct ggatcgacca ctggctgcgg taacaagtcc ggtgcatgaa
    18781 cgctgatggc attcatcact gcgtggcgtc cggcaaaatg ctcttccggc agatagcgac
    18841 aatcggaacg cggataagtg attagcttgt gcgtttcgta caacttctgg cagatatcaa
    18901 gcacgttctg cgcactcaga ccaaagcgtt ttgccgcttc aatctgcaac gccgaaagcg
    18961 aaaacggcag cggtgcggat tctgattccc gtttatcgtt atagctggtg acaatagccg
    19021 gttggccgct gatgcggttg accacatgct ccgccagcgg acgatgtaac aagcgccctt
    19081 cttcatcctg ataaggttca cacgcttcgc tcggttgcca gatagcggta aaccgctcat
    19141 cggcaggtgt cacgatatgc gctttgactt caaagaaatc tttcgccacg aagttttcaa
    19201 tctcttcgtc gcggcgcacc accagtccga gtacgggcgt ctgcacgcgt cccacggaaa
    19261 gtacgccctg ataacccgca ttgcgaccga gaatggtgta agcgcgggtc atattgatgc
    19321 cgtacagcca gtcggctcgg gctcgcgcca gtgcagaaac gcacaacggc acaaactcac
    19381 tgttggaacg aagacggtcg atcgcccgct caaccgcctg cgggttcagg tcgtttatca
    19441 agcaacgctg cacctgctgg cgcttttccg gtgccagttg cagatagtcc agcacttcat
    19501 ccaccagtaa ttgcccttcg cgatccgggt caccggcgtg aacgatttcg ctggcttcat
    19561 gcaggaagcg tttgatgaca ttaagttgtt tggtcacgga gggtcggggc tgtaattgcc
    19621 acttttccgg gacaatcggc aaatcagcaa gattccagcg cgcatagcgg ctgtcgtagg
    19681 cgtctggctg cgcctgctca agcaggtgac cgatacacca ggtcaccacc tgaccattac
    19741 cgcactcgat aaagccatcg cctttccggt gcggtttggg caggacatca gcaatggcgc
    19801 gcgccagact cggtttttcg gcaataaaca accgcattga attaacggat ctcaaccatg
    19861 gcacgaccgc cgcgcgctgg caccagttcg ccaatcgccg tcagttcaat gccaaactcg
    19921 gcggctgtag ctttgacctc attttctgct tccggcatga ccgccagcag cagaccgcca
    19981 gaagtttgcg gatcgcacag cagatcgcgc acttcacgcg gcatttcacc catcagatga
    20041 ccgtagctgg caaagttacg ttcggtgccg ccaggcactg cgcccaactt aatgtactct
    20101 tcaacccccg gcagtttcgg gatcgcttca tagtcgacgc gcgcctgcac accagcaccc
    20161 tgacacattt cgcttaaatg ccccagcaga ccaaagcccg taacgtcggt catcgctttt
    20221 acgccttcga tgttggcaaa ggacgcgcct gcgatgttca tccggcacat cacttccgtc
    20281 gccagtccct gatgttctgg tttcagcagt gattttttct cagccgtggt aagaacgccg
    20341 atccccagcg gtttcgtcag gaacagtttg catccggctt gtgcggtact gtttttcttc
    20401 acccgctcgg tcggtacgat ccccgttacc gccagaccaa aaatcggctc cggcgcatcg
    20461 atggagtgac cgccagccag cgcaataccc gcctgacggc atgcatagcg tccaccttcg
    20521 gttacttcgc gggcaatttc cggggaaagt ttgttaatcg gccagccgag gatcgcaatc
    20581 gccataatcg gtttgccccc catcgcgaag atatcgctga tggcgttagt cgccgcaatg
    20641 cggccaaaat cgaaaggatt atcaacgatc ggcataaaga agtcggtggt actgataacg
    20701 ctggtgccat tgcccagatc gtacaccgcc gcatcgtcgc gggtttcatt acccacaagc
    20761 aaattcggat caacaaactt cgcctgctca ctgtgcagga tggtttccaa cacttttggg
    20821 gaaattttac agccgcaacc agctccgtgg ctgtattggg tcaaacgaat cgagttctcg
    20881 ctcatggaca tctcctgtca atgcaatccg ggtatggtaa ccctcattcc gtgaagtgat
    20941 aagtgagagt gtctgaattc ctgcgccttt gctcacaatc cagacagttt cgcgacaatt
    21001 atcagaaata agtgacaaac ggcgtcgggt ccgggacgtt aatcgacgta gatgctttca
    21061 gctgcggcgt accaaggtag agaaaaccga caattttatc ctgctcacgg caaccgaatg
    21121 cttcacgcac taccggactt tcagttaatg cgccactgcg ccagatgccg ccaaacccct
    21181 gggcaactgc tgccatttgc atcgccatga ccgcgcatcc cgcagacatt tcctgttccc
    21241 agcgcgggac tttatgattt tcttcgcatt ttgccaccac cgttatgatg agcggtgcgc
    21301 ggtacggcgc attacgggct ttgtcgatgg ctttgtcatc actaccgcca gcaatcgccc
    21361 cctgctccag aacggcgctg aaacgctcgc gcccttcccc ttcaatcaca aaaaaatgcc
    21421 acggttgcat ggacttatgg tccggcgcac gcatacccgc acgcaggatg ttttgcagtt
    21481 gttcacccgt tggcgcaggt tcagccaggc gggaggcgct acggcgattg atcaatagtt
    21541 cgagtgcatc catttgatta actcctgtcg tgatatttat tcacaaaatt aacacgagag
    21601 tggattttgt tacagcccag tccgcaattc ctgctgacaa gtgccggttg ggtcattacg
    21661 ataaccacat ctattgcgcc tgtgacaggt gtgaccttta gttgggagaa tacatgcgaa
    21721 ccctttggcg atttattgcc ggatttttta aatggacgtg gcgtctgctg aatttcgtcc
    21781 gtgaaatggt acttaacctg ttctttattt ttctcgtact ggttggtgtg gggatttgga
    21841 tgcaggtcag tggtggtgat tcgaaagaaa cggccagtcg tggcgcactg ctgctggaca
    21901 tttctggtgt gatcgtcgat aaacccgaca gttctcagcg gtttagtaaa ttaagccgcc
    21961 aactgcttgg tgccagttcc gatcgtctgc aggaaaactc actgtttgat atcgtcaata
    22021 ccattcgcca ggcgaaggat gaccgcaata tcaccggtat cgtgatggat ctgaaaaact
    22081 tcgcaggtgg cgaccagccg tctatgcagt acatcggcaa agctctgaaa gagtttcgtg
    22141 acagcgggaa accggtttat gccgttggcg agaactacag ccaggggcaa tattatctcg
    22201 ccagtttcgc caataaaatt tggctgtcac cgcaaggcgt ggtggatctg cacggctttg
    22261 ccaccaacgg tctgtactac aaatcgttgc tggataagct gaaagtttcc acccatgtgt
    22321 tccgcgtggg tacgtataaa tctgccgttg aaccgtttat tcgtgatgat atgtcgcctg
    22381 cagcccgcga agctgacagc cgctggattg gtgagctgtg gcaaaactat ctgaatactg
    22441 ttgccgctaa ccggcagatc cctgctcagc aggtattccc tggcgcgcaa ggggtgcttg
    22501 agggtttaac caaaaccggt ggcgataccg cgaaatatgc gctggaaaac aagctggtcg
    22561 atgcactggc atcgagtgcc gaaatcgaaa aaactctgac caaagagttc ggctggagta
    22621 aaactgataa aaattatcgc gccatcagtt attacgatta cgcattgaaa acgccggcag
    22681 ataccggtga cagtatcggt gtcgtcttcg ctaatggcgc aattatggat ggcgaggaaa
    22741 ctcaggggaa tgttggcggt gataccactg cggcacaaat ccgcgacgct cgccttgacc
    22801 cgaaagtgaa agcgattgtc ctgcgtgtta atagcccagg cggcagcgtt accgcgtctg
    22861 aagtgattcg cgctgaactg gcagcagccc gggcagcggg taagcctgtg gttgtatcga
    22921 tgggtggcat ggcagcatct ggtggttact ggatttccac gccagctaat tacattgtgg
    22981 ctaaccccag caccctgacc ggttctatcg gtatcttcgg cgtgatcacc accgtagaaa
    23041 atagtctgga ttcgattggt gttcatactg atggtgtctc aacttcaccg ctggcggatg
    23101 tttctatcac cagggcactg ccgccggaag cgcagcagat gatgcagtta agcattgaga
    23161 atggctataa acgctttatc acgctggttg ctgatgcgcg tcattcgacg ccggagcaga
    23221 ttgataaaat cgcccagggc cacgtctgga ccggtcagga tgcaaaagct aacgggctgg
    23281 tcgatagtct cggggatttc gatgatgcgg tcgccaaagc agcagagctg gcaaaagtga
    23341 aacagtggca tctggaatac tacgttgatg aaccgacctt cttcgacaaa gtgatggaca
    23401 acatgtctgg ttctgtccgg gcaatgttgc cagatgcgtt ccaggccatg ttacctgcac
    23461 cgctggcctc ggtagcctct accgttaaaa gtgaaagcga caagctggcc gcgtttaacg
    23521 acccacaaaa ccgttatgcg ttttgcctga cctgcgccaa cgtgcgttaa gtcttgtact
    23581 gagtggccga cagatcgtcg gccacatttt ttacctcgac gaatcctctt cccgctgtct
    23641 cgccccatat ttccttatcc gcatagtatc aggtgcgctc cccctgcctc acgtatatac
    23701 ttttgctctt tcgatatcat tcatatcaat atcatgcaaa agaaatcaat ttacgtcgcc
    23761 tacacgggcg ggaccatcgg gatgcagcgt tccgagcagg gttatattcc ggtgtcaggt
    23821 catctacagc gccaactggc gctgatgccg gaattccatc gcccggagat gccagatttc
    23881 accattcatg aatatacgcc gctgatggac tcttcagata tgacgccaga agactggcag
    23941 catattgctg aagatattaa agcgcattat gacgactatg atggttttgt cattctgcac
    24001 ggcaccgaca cgatggcgta taccgcctct gcgctgtcgt tcatgctcga gaatctcggt
    24061 aaaccggtca ttgtgacagg gtcacaaatc ccgctagctg agttacgctc tgacggacaa
    24121 attaatctgc tgaatgcgtt gtacgttgcg gcgaattatc ctatcaacga agtaactctc
    24181 ttttttaata atcgacttta tcgtggcaac cgtaccacca aagcccatgc cgatggtttt
    24241 gatgcgtttg cctctccaaa ccttcctccg ttactggaag caggtatcca tattcgtcgt
    24301 ttgaatacgc cacccgcccc gcacggtgaa ggggaattga tcgttcatcc aatcacccca
    24361 caaccgattg gcgtagtgac gatttatcca gggatttctg ctgacgtcgt gcgcaatttt
    24421 ctgcgccaac cggtgaaagc attgattctg cgctcatatg gcgtgggtaa tgcgccacaa
    24481 aacaaagcct tcctgcagga attacaagaa gccagcgatc gcggtattgt ggtggtcaac
    24541 ctgacacaat gtatgtccgg taaagtgaac atgggtggtt atgccaccgg taacgccctc
    24601 gcccatgccg gcgttattgg cggtgcagat atgactgtag aagccacact aaccaaactg
    24661 cattacctgc ttagccagga actggatact gaaaccattc gcaaggccat gagccaaaac
    24721 ctgcgcggcg aactgacgcc ggatgattaa ggagactgca atgccccctc gcgccctgtt
    24781 actggtcgat ttacaaaatg atttctgtgc tggtggcgcg ctcgccgtgc cggaaggtga
    24841 cagtacggtg gatgtcgcta accgcctgat tgactggtgc cagtcgcgcg gtgaagcggt
    24901 tatcgccagt caggactggc acccggcgaa tcacggcagt tttgccagtc agcacggtgt
    24961 agagccttat acgccaggcc aactcgacgg tttgccacaa accttctggc cagatcactg
    25021 tgtgcagaac agtgaaggcg cacaattaca tccgttactg aaccaaaaag cgatcgcagc
    25081 ggtgttccat aaaggcgaaa atcctttagt tgacagttac agtgcctttt ttgataacgg
    25141 ccgtcggcag aaaaccacgc tcgatgactg gttacgcgat catgaaatcg atgaattgat
    25201 cattatgggc ctggctactg actattgcgt gaagtttacc gtactggacg cgttacagtt
    25261 aggttataag gttaatgtga ttaccgatgg ttgtcgtggt gtgaatatcc agccacagga
    25321 cagtgcgcac gcgtttatgg agatgtcagc agctggggca acgctatata cgctggcaga
    25381 ctgggaagag acgcaggagt aattttatgc tggcctacaa ttctgtactg ccattgtagg
    25441 ccagataaaa cacgtcagtg gtacatctgg caattgacgc catcaacgaa agatttaatt
    25501 cacctcagaa atctcttcca gcgacacctt ccgcgtttcg atgccaaaaa tggagagaac
    25561 cagcgcacag agcactaaca tcacgccaag caccataaac accgtgattg acccataatg
    25621 cgttaatagt gccgcaacgc cataaggcgt gaagaccgcg acgatccgtc cgacggcatt
    25681 aacgaaaccc gaaccgcgca agcgtaaatg cgttggccag agctccggga tataaaccgc
    25741 cgacgcgaag caaacataca tgtataaaaa gaagatcatc accaggccat agattaaaat
    25801 cgcccactct gtagtctgaa ttgaatagat atagcctaac acggcaataa taataagtaa
    25861 ggcggagcca aataatcgac gaggaaaatg atcaataatt aatgccgcaa taaatattcc
    25921 taccggagcg ccaatcataa taacagcagt cattaatatt gatttatcga catcaatgcc
    25981 ggagttaaca aatatggtcg gtatccatac ggtgatggta taaagtgaaa tattcattgc
    26041 aattaaaaca gtaatcgcga ctaatgtacg tcgtaacatt tcccctttaa acagcagcca
    26101 gaaagtacca ttgattacgt tagccttgct gttgctctga ttcaaagtaa gcgggggtaa
    26161 acgaacactc ttctctcttt caatttgctg ctctacttca cgaagttggc tttctgcacc
    26221 tgcgatttgt cctttccccg ccaaccatcg aggcgactca ataaagtatt tacctgagag
    26281 aaaccaggct aacagtatga caataccacc cagcagaaac attatccgcc aactaaaaaa
    26341 agcgataacc accacgccta tcgccgcaga caacatgggc gaccagttac cgacaaatga
    26401 gagccgcgcg gaccattttc cacgcaccgt cgcggggata aactccgtaa acgaagcata
    26461 gccaaccata atcagcgccc ccattcctgt gcccatcagg aagcgaaaga agatgagcca
    26521 gtacatatca gggacaaaag cggcccctgt tgccgcaata ccgacgatga gaagatttat
    26581 gcgaaaagcc ctgcgccgcc caaaataatc accgatgaac ccaccggtaa gagagccgat
    26641 gaaataacca aacattaatg ccgaggtaaa ggcggcattg aggaaattat ttgaccatcc
    26701 attgcttacc agctttgcta aaacaacatt gcctgaataa ctcaaaaaac ccgttaataa
    26761 cagactaaag cttataatac caaatatacg ataatgaaac cgggccaaag gcaagcggtc
    26821 cagtcttgcg ccaatttgat catattgttc cattaggtgc ctcggatttg ctaaaaaggt
    26881 agcaattgcg gcaaattcac ttgccgcatc tttatcatca ttttattttc caggcgcagg
    26941 aacaaataat attgacaacg tgtgcttaaa ttaccacacc agctggatat tattgtcttt
    27001 gcaaaatgct atccactctg cacccggtga tttatcagtt attatgtaat taatatgact
    27061 aaagtcagct aactggacaa aagctttgcg atcaaactta gagtgatcaa ccaataacgc
    27121 aacctctgtc gcctgacgga tcattgtctt tttgatttca gcttccgctt cgttagagtc
    27181 cagcgcgccg ctgttaatat caagaccttt acaactcatt accatgatat cgacatggta
    27241 gcggctgatg atctctttag taattcttcc ttgcagggaa agtgtatttt tgtttagttc
    27301 cccccccgtt gaaacgactt taatttctga ttgagccaga acatgaatcg cttccgccga
    27361 gttagttagc aacgtcaggt cactacggtc ctgcaacaat ttgagcaatt ccataacggt
    27421 actactggaa tcggctgcca tagtggtttt attgtcgata aagggtagtg ccttgcgtgc
    27481 aataagctgc ttctcttcat aaaacgatga agcgcgctta taaaaatgga tattctccgt
    27541 caacatcgct gtatttaaaa cagcaccacc ataggttctg gtcaaaaagc cttcatcttc
    27601 cagcttctcg agatcgcggc gaatggtttc ttcggttacc tgaaaaatcc cactcaaatt
    27661 tgagactgtc acctttttat cgttggcaac catttgctta attgcctgaa tcctgtcttt
    27721 tgccgccacg attacacccc tgtatctttt tacatcacat tagcgcgatt atcgcataac
    27781 cgatgtttac tttcaaaata acctgtttga atcacagatt ttcatcacag ttttcacaga
    27841 aacagaggtg aatcgtgttg agtatttaac gctccagggc ctctgccatt tccctcatca
    27901 atgttgcgtc tgcatccgat aagttgatat tcaatgccgc gacattttcg cgtacctgtt
    27961 ccggtgcagt agccccacta agaatggaga ttaaatcact ctgttttaat atccacgcca
    28021 gtgccagagt gggaattgtg cactgataac gagcacagag tggctgccac tgttcaagca
    28081 tatcaatcac tttcagcatg ttttcacgct ggaaccagac tttatttgcc cgagcgccgc
    28141 ccggaacgta atcacgagtg atggtaccgg tcaacaatcc ctgctctaac ggcgaataaa
    28201 cctgtaccac aatgccatta tcacgacata gcggcagcag ttcgctttcc attgcccggt
    28261 cgaggatact gtatttcgcc tgaataatat ccagttcacc atattgcaga tactcgcgga
    28321 tatggtcagc atcgacgtta gcagcgccta tagcgcgaat tttcccttcg tcctttaact
    28381 cattgagcac tgcgacagtt tcagcgatcg gcgtaaaaaa tggcggcacc gactgccagt
    28441 gcgtcatgta gatatcgatg taatcaatac ccagacgttg caagctggct tctacctctt
    28501 tgcggataga ttccggggaa aggtttttat acaactgccg atcgccaact ttgttgaata
    28561 aactcccttt tcgttcccag acaatgccgc atttggtttc tactacaacc tgttcacggg
    28621 gcagtttttt taacgcctga ccgacgataa cttcactatt gccaaagtta tatcctggcg
    28681 cagtatcaat caggttaatg ccgcaacgat gggcttcaag aatcgtatca atacatattt
    28741 gtcgatcgag atcgccattc catgcaggac cgccgccaat ggcccatgtc cccaacccca
    28801 ttcgcgaaag cgtaatatcc gttgtgccta aaggtatctt tttcatttgc cttcattatc
    28861 cttcgcattc ttccagcaat tgttcgacca gctttctgtt ttttacgccg gtggaggcac
    28921 cgacgcttag aaccgagata gccgccgttg cattggcaaa gcgtgcgcat tcacgcagat
    28981 ttttgccttc taacagtgcc gcaataaaac ctgaagcaaa gttatcgccc gcgccaatgg
    29041 tgtcgatggc ggttattcct gcgaccgccg gcaccttcat cgtcatgtca ccacgcttga
    29101 taaagcagcc gtctttaccc gttttaatca ccaccgtttt tacgccgcac gcaagaaagc
    29161 agtcagcaat ttcatccagt gtctctttcc cggtgagtaa ttttgcctcg gcataattag
    29221 gaaacagata atcgacataa ctcaatgctt cgcaaatatc atccagcgtt tcattcaacc
    29281 gcggtttgat catatcggca cagataatca tctgccgggc tttagcttgc gtaaaaattt
    29341 ctgttagcgc tttaccatcc aatagaggac tgttgaaaat actggccaga gataataatt
    29401 tcgcctgaga aaaccgcgaa aaatcaacat cgtcaatatt caatttccac agactgccgt
    29461 tacagtttgt gacaaacgtc cgctcgccat cctccgtcac cagtccaacg ttaattgagg
    29521 tatctatgct gacgtcctgc ttcaggcttt gaatatcaat attctctttg cggcaatggt
    29581 cgagaataaa ttgcccggcg gcatctttac caatacgact cattaatgct gtgcgatggc
    29641 ccagacgaga aataattgtt gcttcattta ttgcatcacc cccggtggtc attgcgattc
    29701 tttcaagagg ataagaatcc acatcaaaga tatttttact gaccggttgc aatggaatat
    29761 caacaatagc ggcacctata caaataacgt cgagattatc catatcatta ttccgctttg
    29821 ccatcagaac cgaacagttt aattttttcc aatgcccgtt cttttacagc tttacgtact
    29881 tcacgttcta aatgcaggaa aggctgatcc tggttttctt tcactgctac cattgcggcc
    29941 tgacagagct ccgtatgaat gttgattttc gcgataccca acgaaattgc agttttaatg
    30001 tcggtatcac tgatccccga tgcgccgtgc aaaaccagcg gaacagaaac ggcatcgcgt
    30061 acgcgtttga cgacctcaaa attcaattgt ggctctgacg tataaacccc atgttggttg
    30121 ccgatggcga cagccaatga gtcacagccc gtacgttcaa caaattcagc cgcctggtca
    30181 ggatcggtat agtgatagcc cgccagcgct tcttcataaa cggtttcatt accgacatgc
    30241 cctaattctg cctctaccgg aatacccagc ggatggaaga aatcaacagc ctctttggtt
    30301 aaacgaatat tttcttcgaa atcaaatgcg gaagcatcac gcattaatga attcatacca
    30361 tgagtccagg cgttatgaat aatttccata ctccgaccat gatcccaatg agttattacc
    30421 ggcaccgttg ctttttttgc cattgatacc atcatgtgag agaaatcttc aaatgaggtg
    30481 ttcccgacaa aacctgtacc aaaagaaata ataaccggtg atttcgcttc ttcggctgcg
    30541 tcgataacgc ccatcaacat ttctgcattc catacgttaa aatgggcaat tgcataatgt
    30601 ttatttgtgg catcgttttc ccaatatctg atatctgcga gcatattctg ttctcccgta
    30661 ttaattaatc gctaatttta ataacgcctt taataatgtc gcgtttgttg ttaaccgact
    30721 cttcaaatgc ctgttgtaca tcctgataat cgtaaatatg tgtcaccatc gatttcacat
    30781 cgaatcgccc tgaggaaata gcttcaatcg tgaccggata acgattggca tagcggaata
    30841 ctgtctgaat agtgacttcg cgattgattt tgaggaaatt gattgccgaa tcgcccggta
    30901 cagtaccaac aatcataatt ttaccgccgc gcattaccag ataaggtgcc tgtttaacgg
    30961 tgaccgcaga acccgctgtt tcgaaaacaa tatctgcgcc catttcttcg gtaaattgct
    31021 gacagcgtgc aatagtgtct tcttttgcac cgttaataac cactgtcgcg ccgagctgtt
    31081 ccgccattgt cagacgttta tccagtacat caacgacggc aatatccgta gctcccaggc
    31141 atttgcacgc ttgcaacgtc atcaaaccaa tacaacccgc tcccagaata attatcttct
    31201 tacccggttt aacatctgcc agcatcgcgg catgcatccc gactgcagca ggctccacca
    31261 gcgccccttc catcatgtcc atattgtcgg gcagtttgta agtaaagctc tccggatgac
    31321 acagatagtg cgttaatgcg ccgcggtagt taggttgtgt cgccataaaa tcaacgtccg
    31381 ggcagatgtt atatttgcct tccagacagt aacgacagtg accgcaagga acgccaggtt
    31441 cgatatttac ccgatccccc gctttaaatt tgcgcacacg gcttcccaca gccacaaccg
    31501 tcccggcgca ttcatgaccc aagccaattt cttgatttgg gtctttaggc ggaataaacg
    31561 ggcctgattc aaaaccatgt acatctgaac cacaaatacc gacatattcc actttaatca
    31621 aaacttcatc ttctttaggc acaggtattt ctgctgaaat aattttcatt gtgcccggca
    31681 cctgcaatat tgcttttgaa tttttcattt tgaatccacc agtattattt attgactact
    31741 gcatcaatac ttaaactttc aacggagaca cctttggttt caattccaat ggtggcgata
    31801 gcaattgcga caataattga aaccgccccc agaagaataa agactcccgt tacgccataa
    31861 ctactgagca gcactgcaac ggcataaggt gcggcaatac cactgattcg ccctactgca
    31921 ttcgccagac cggagccacg taatttggcc tctgtcggcc agatctcagg gacatacact
    31981 gccgaggcat agcaaacgta catatagacg aaagtaatca ggaagaaacc aatcaacgtt
    32041 atgagcaaca tactggtttg cagtgaatag atatatccga gcaccgcaat caggattaat
    32101 agccccacac ccattgtttt acgcggaatt ttatccatca ccagcatagc aataaaaata
    32161 ccaaatggcg cgccaaacat actcatggta tttaaaacaa tcgagtcttt taaattaatc
    32221 ccctgggtca tgaatattgt tggcaaccag ttaattagtg tgtactgcac aacgttcatg
    32281 gcaatcagca cacaagagcc taatatcacg cgtttcagta atactcctgt cattaaggct
    32341 gaatacggca ccgcttgtgg cgctttaccg tcatcagcaa taaccacggg cggcaaaggt
    32401 tttcccgtct ggcgtatgac gccttcttct atactccgca tgactttctc ggcttcctga
    32461 taccgtccgc gcgattccag ccagcgcggg gattcaggaa agtagcgcca ggccagcgcc
    32521 gtagcgataa gcgacaatat tgcagggata agcagttgta ctcgccagtt ccactctgca
    32581 ctaatcagcg gcgtgagtcc catcgcaatc aatgaacaga gcggatatga ccagttgcca
    32641 ataaaagaaa cccgactgga ccacgttccg cgatttctac cgggcatata ttcggtgaaa
    32701 ccagcaaaca gcgtcaccag taaagctccc agcccaacgc ccatcacaaa acggcaggca
    32761 ataaggaaat ccatattcgg tgaaaaagca ccgaccacca tcgaggcaat atgaatggcc
    32821 tcatagagga tgaacgcatt tctgcgaccg gttttgtcac cgatgatgcc gccaaccagc
    32881 gcaccgagaa acattccggc ggtcgtgatt gctgagaatg tggcagtggt ggaattatct
    32941 gtccagccca actctttcag ctgcgcgagg atcaagccac caacggcatt actccagcag
    33001 acaagcaagc caaacgagac gatggcaaac attgacgaat gccagcggca atccggtaag
    33061 cgatccagcc gtgcaccaca atgcggtttt gttatctgtt ccatgcgata acgttccttc
    33121 aggagagtag ttattcatca aagtcgtaag tcatgatcac tttgattgcg gttttatcga
    33181 ccatcgcatc aaacccttcg cgccattgcg acaggccgat acgatgcgtg atcatcggtt
    33241 tgactttgat agcgccgctg gctaatagcc ggagagcgtt acgccatgag gtggagtcat
    33301 aggccatatg cccaatgatg cttttgttcc aggcggtaat gtcattaatc gagaaatcaa
    33361 gaggtttgaa gcccattcca acgcgtacca cttccccgtt cggacggagc atttcgatgg
    33421 cttgtttcag tgcgatattg gcaccggagc attcaatcac cagtcccaga ttgtctttgc
    33481 cacaaatttg ctggcagcgc gccaccacat cttcggtaga accatttact actgccgtcg
    33541 cacccagttc ttttgcaacc gggaagcgga ccgccacatc ttcttgtaga ccaacgacga
    33601 cgatatttac cgcccccata atccgcgcca tttgtacgga gaacagcccg agtgggccag
    33661 tgccgatgac gaccacatcc tgaccaggga ggaatttcga ttgctgcgcg atggatttat
    33721 aggcattaca gatagggtca agtatggctg cgtcctcata atcaacacca tcagggattt
    33781 cccacaatgc atgacgatga attttgagaa tttcaccagg aaccagacaa tatttggaaa
    33841 aaccaccgcc ccaggtattg ttatccagac caaggtttac tttttctgta caacacagaa
    33901 aatcaccttg ttcacaggcc ggacaaacgc cgcagacgtg accgctgtta tccgacacga
    33961 cgcgttgccc cactttccag tctttgactt tttcaccaac ctgcgcaata caacctgcga
    34021 actcatggcc gcggatagag ttaaactcat cagaaccgct atcgacattg tagtgcttca
    34081 tgtctgcgcc gcagattgcc gcggctttaa tttcaatcac tacatcttcc gggccacaaa
    34141 tgggttgtgg gacatcgatc atcttgtagc cgccaaaggc tttgccaaac cgagccagtg
    34201 ctttcattgc gtcatcctct gtgttttgtt ttgcttagat ggtcatcata cttacccaca
    34261 tgatttttga ggtcaacatt aaatctgtaa aacacagata atgatctgcg ttttacaact
    34321 cagatcacaa ttacgcagaa aatggctgga tctgttacag gttgatggaa ggcggaacgc
    34381 aaaatgagca aatttgagag ggattaccgg ccaaataggt ctgagactta agttcaggag
    34441 aagtcattca ggcgtgaacg cgactgcaag agaccgggct gggacttagc ctggtcgtga
    34501 cgagataatt gagatgagtc gtggggttat tgttggctat cccctttata accgcggcaa
    34561 aaaggggaca aaccacttac ttaaacatgg caatagcctt tgctgagata ccaaaatctt
    34621 cttttaactg ttgtttgctc ttcatgacca tctgaccatt tgtgtcaatt gtcatatgct
    34681 gtgcttcggt attattgcgg gcttgccaca gcatcaccag ttgcaggcag ttctcttttt
    34741 gttcctcggt caacgcaacg ccatcaggcc atttgcccaa ttcaacggcg gtcgacaaac
    34801 gctggtatac ctcaagcgtc atgctgttga taatgtcatc aagattcata atttttccgc
    34861 tcctgtggaa taagttgctg aatcgttttt tcaaccgttg atttcttcgc cgttttcgcc
    34921 atcggtaaag cgtaaagagg ctgagttaac acaataacgt tcacccgttg gctgcggccc
    34981 gtcggggaag acatgcccca gatgggcatc acagttaccg caacgaattt ctatgcgctg
    35041 cattccatgt gacaagtctt tgatataacg aatggattct tcactcaccg gttcgtagaa
    35101 actgggccag ccacagccgg aatcatactt ggtttgggaa tgaaacagcg gggcatcgca
    35161 gatcaaacag tgatatacgc cgtcacgctt gttatgcagt aaacgacccg taaatggcgg
    35221 ctctgtccca tgattctgcg tcacgtagaa ctgcatctcg gacaaatttt ttttcagttc
    35281 ttctgccgaa ggtttattag ccatttgctc acatctcact ttaatcgtgc tcacattacg
    35341 tgactgattc taacaaaaca ttaacaccaa ctggcaaaaa tttgtcctaa acttgatctc
    35401 gacgaaatgg ctgcacctaa atcgtgatga aaatcacatt tttatcgtaa ttgcccttta
    35461 aaattcggga cgccgacccc atgtggtctc aagcccaaag gaagagtgag gcgagtcagt
    35521 cgcgtaatgc ttaggcacag gattgatttg tcgcaatgat tgacacgatt ccgcttgacg
    35581 ctgcgtaagg tttttgtaat tttacaggca accttttatt cactaacaaa tagctggtgg
    35641 aatatatgac tatcaaagta ggtatcaacg gttttggccg tatcggtcgc attgttttcc
    35701 gtgctgctca gaaacgttct gacatcgaga tcgttgcaat caacgacctg ttagacgctg
    35761 attacatggc atacatgctg aaatatgact ccactcacgg ccgtttcgac ggtaccgttg
    35821 aagtgaaaga cggtcatctg atcgttaacg gtaaaaaaat ccgtgttacc gctgaacgtg
    35881 atccggctaa cctgaaatgg gacgaagttg gtgttgacgt tgtcgctgaa gcaactggtc
    35941 tgttcctgac tgacgaaact gctcgtaaac acatcaccgc tggtgcgaag aaagtggtta
    36001 tgactggtcc gtctaaagac aacactccga tgttcgttaa aggcgctaac ttcgacaaat
    36061 atgctggcca ggacatcgtt tccaacgctt cctgcaccac caactgcctg gctccgctgg
    36121 ctaaagttat caacgataac ttcggcatca tcgaaggtct gatgaccacc gttcacgcta
    36181 ctaccgctac tcagaaaacc gttgatggtc cgtctcacaa agactggcgc ggcggccgcg
    36241 gtgcttccca gaacatcatc ccgtcctcta ccggtgcagc taaagctgta ggtaaagtac
    36301 tgccagaact gaatggcaaa ctgactggta tggcgttccg cgttccgacc ccgaacgtat
    36361 ccgtagttga cctgaccgtt cgtctggaaa aagctgcaac ttacgagcag atcaaagctg
    36421 ccgttaaagc tgctgctgaa ggcgaaatga aaggcgttct gggctacacc gaagatgacg
    36481 tagtatctac cgatttcaac ggcgaagttt gcacttccgt attcgatgct aaagctggta
    36541 tcgctctgaa cgacaacttc gtgaaactgg tatcctggta cgacaacgaa accggttact
    36601 ccaacaaagt tctggacctg atcgctcaca tctccaaata agttgagatg acactgtgat
    36661 ctaaaaagag cgacttcggt cgctcttttt tttacctgat aaaatgaagt taaaggactg
    36721 cgtcatgatt aagaaaattt ttgcccttcc ggtcatcgaa caaatctccc ctgtcctctc
    36781 ccgtcgtaaa ctggatgaac tggacctcat tgtggtcgat catccccagg taaaagcctc
    36841 ttttgcatta cagggcgcac accttctctc atggaaacct gcgggtgaag aagaagttct
    36901 gtggttgagt aacaacacac cgttcaaaaa tggcgtcgct attcgcggtg gcgtaccggt
    36961 ttgctggccg tggtttggtc cggctgcaca acaaggcctg cctgcgcacg gttttgcccg
    37021 caacctgccg tggacgctga aatcacatca tgaagatgct gatggcgtag cgctgacttt
    37081 tgaattgacg caaagcgaag agacgaaaaa attctggccg cacgacttta cgctgttagc
    37141 gtatttccgc gtgggtaaaa cctgtgaaat cgatcttgaa gcacacggcg aatttgaaac
    37201 cacctctgcc ctgcatacct actttaacgt gggtgatatc gctaaggtaa gcgtcagtgg
    37261 gctgggcgat cgcttcattg ataaagtgaa tgacgcgaaa gaagatgtac tgaccgacgg
    37321 tattcagacc ttccctgacc gtaccgatcg cgtgtatctg aatccgcaag attgcagcgt
    37381 gattaatgat gaagcgctga atcgtattat cgccgtaggt caccagcatc atctgaacgt
    37441 tgtaggctgg aatccgggac cggcgctttc agttagcatg ggcgacatgc cggacgatgg
    37501 ctacaagacc tttgtttgtg tagaaacggc ttacgcttca gaaacgcaaa aagtcaccaa
    37561 agaaaaacct gcacatctgg cgcaatccat tcgcgttgcc aaacgttaat ttgcgttaat
    37621 gttgtgtgcc gggtgcgatg catccggcac gtatcaacat cacaccatat ccagcgcagt
    37681 ttttcctttt ggtgccggat acgctttatc cagcatagct aattccgctg gagaaagttc
    37741 gacctcaagc acagccgcat tctgttggac atgggcaatc gtggccgctt ttggaatcgc
    37801 catcacaccc tgatggctga ttacccacgc caacaatact tgtgccgagc tgatattgtg
    37861 agcatgtgca atttcgttga ctactgcgtt ttttagcagc ccattgcgca accgcccggc
    37921 ctgagctaac ggactgtaag ccatcaccgg tatctgctgt tgctggcacc agggaagtag
    37981 atcgtactca atccctcgtg aaccgagatg gtaaagtacc tgattagtgg cacactgatt
    38041 tccccccggc agctgccaga gttcctgcat atcggcataa tcaaggttag aaacgcccca
    38101 gcggcggatt ttgccctggg cgaccaattt gtccatcgct gcgacagtct cttcgagagc
    38161 gaaactgcca gaccagtgta ataagtagag atcgagataa tcagtattga gacggcgtaa
    38221 actggcttcg catgcattta tcgctttttg cccgccagca ttccacggat agactttaga
    38281 gacgagaaag accttctctc gcagaccggt taatgcttcc ccaaccacct tttcggcacc
    38341 accatcggca tacatttcgg cggtatcaat gagggttaaa ccgagttcaa tgcccgcgcg
    38401 tagtgcagct acttctgttt tgcgctgact ggcatcttcg cccatatacc atgttccctg
    38461 ccctatggct ggcagtgaga catcgccact aaattgaatc attttttgtt gcattgtttc
    38521 ctcccaggta ttgcaccacc gtaatgcaaa acagggcgtg acgccctgtt tttatgcaca
    38581 aaatgccctg gaaagatgca ttatcagaat ttgtaggtga tcccggtaga aatcaggcca
    38641 gtccaggatt tatccaccat cgggctgtca gtaacttcat cagacagacg ggtgtagcgc
    38701 gcggtaccgt aaacactcca gtcgccgagg aagttgtagc tggcgctcag ctccaggtaa
    38761 gggctccagc tgtcgttcgg gttatagcca cgcagaccgc tgcgagcgga ctctttgcgc
    38821 gatacgccat aatagtattc gttctggttt tcgctgttcc actgcacacc aatacccgga
    38881 gtcacggtca ggccgccgtt ggtgtaacga tacaaccagg ccatatccca gacaatgccg
    38941 ttgctgttat ccagggtatc gccagccagg gtggtacgca ggtaaccgta ctgggtaaag
    39001 tgagcataag acagaccagc catcatggtg ctcttacggt catccaggtg acgcatttgg
    39061 tgatcgccac tgtctttcgc tttgaagtaa agcggcgacc agtaagcggt aattgaaagt
    39121 ttgtccgttg cgtcattcca caggtagtaa ccaccaccta agccacggaa ccagaagtta
    39181 tcgccttcat agttgattac cggtactggg taaacatcgg tatcgtaatc tttatatggg
    39241 tgttcaacga cacctacgcc tgcgcccagg gaaaatttac cttcagcgtg cgctacgcct
    39301 gcagacgttg cgataagcac tccaagtgcc agaagtttga gtttggtcac aattaatcat
    39361 tccttaaaca aatgtttagc gggcgacaaa gtttacccgt caatacatcg aaacccaacc
    39421 tttttacgtt ttcatttttt aaagtaactg tttaattttc ctgacgcgga tgacaccgcg
    39481 cttacagcca aatgaattta gcgttactgg cgaccctggt ctttacatta attatgcaaa
    39541 atttatggat gagttgttga tatgccattg aaattaagaa agccgtgcag gcaagttttc
    39601 catttgccat ctacgcttaa ttttgaaggt gtatcaccgg gcacgttgtt ctcatcgtcg
    39661 ataaaatggc atgagagttg ctgtgtttta gcaagagacg tcgttcagtt tacctcttcc
    39721 gggagcctct actattcata tgaacggctc ttaacctgtg ctaaaaaacg aaaggacggc
    39781 ataccatgaa tatattcgat cactatcgcc agcgatatga agctgccaag gacgaagagt
    39841 tcacactgca ggagtttctt accacttgtc ggcaagatcg cagtgcttat gccaacgcgg
    39901 ctgagcggct attgatggct atcggtgagc ctgtcatggt cgatacagcc caggaaccca
    39961 gactttctcg actcttttct aaccgggtca ttgcacgtta tccggcgttt gaagagtttt
    40021 acggcatgga agacgcgatt gaacagattg tctcttatct gaaacacgcg gctcaggggc
    40081 tggaagagaa gaaacaaatc ctgtatctgc tggggcctgt gggtgggggt aaatcatcgc
    40141 ttgctgagcg actgaaatca ttaatgcagc tcgtaccgat ttatgtattg agcgccaacg
    40201 gtgagcgtag cccggtcaac gatcatccgt tctgtctttt caatccgcag gaagatgcgc
    40261 agattctgga aaaagagtat ggcattcctc gccgttatct cggcaccatc atgtcgccgt
    40321 gggcggcaaa acgcctgcat gaatttggtg gcgatatcac taagttccgg gtagtgaagg
    40381 tctggccgtc aattctgcaa caaattgcta tcgccaaaac ggaacccggt gatgagaaca
    40441 accaggacat ctccgcgctg gttgggaaag tcgatattcg taaactcgaa cactacgcgc
    40501 agaatgaccc ggacgcctac ggctattccg gtgcactgtg ccgcgccaac caggggatca
    40561 tggaattcgt tgagatgttt aaagcaccga ttaaagtgct gcatcccttg ctaaccgcca
    40621 ctcaggaagg taactacaac gggacggaag gtatctccgc cctgccgttc aacgggatta
    40681 ttctcgccca ctcgaacgaa tccgaatggg tcactttccg taataacaaa aacaacgaag
    40741 ccttccttga tcgtgtttac atcgtgaagg tgccgtattg cttgcgcatt tccgaagaga
    40801 tcaaaatcta cgagaaattg cttaatcaca gtgaattgac tcacgccccc tgcgcccctg
    40861 gcacgctgga aacgctgtca cgtttttcca ttctttcgcg ccttaaagag ccagaaaact
    40921 ccagcattta ttcaaagatg cgggtttatg atggcgaaag tctgaaagac acagatccca
    40981 aagccaagtc gtatcaggaa tatcgtgact acgccggtgt cgatgaaggg atgaacggtc
    41041 tgtcgacgcg ttttgcgttt aagatcctct cccgcgtgtt caacttcgat catgtagaag
    41101 tggcggcaaa cccggtccat ctgttctacg tcctggaaca gcagatcgag cgcgagcagt
    41161 tcccgcaaga gcaggcagaa cgctatctgg agttcctgaa aggttatctg atcccgaaat
    41221 atgccgagtt tatcggcaaa gagatccaga cggcctacct tgaatcctat tccgaatatg
    41281 ggcaaaacat tttcgaccgt tatgttacct acgcggattt ctggattcag gatcaggagt
    41341 atcgcgatcc ggataccggg cagctgtttg atcgcgagtc tcttaacgcc gagctggaga
    41401 aaatcgagaa accggcgggg atcagtaatc caaaagattt ccgcaacgag attgttaact
    41461 tcgtactgcg cgccagagcg aataacagcg gacgcaatcc gaactggacc agctatgaaa
    41521 aactgcgcac ggttattgag aagaaaatgt tctccaatac cgaggagctg ttgccggtta
    41581 tttcgtttaa cgccaaaacg tcaaccgacg agcaaaagaa acacgacgat tttgtcgacc
    41641 gtatgatgga aaaaggctac acccgtaaac aggtgcgttt actgtgcgaa tggtatttgc
    41701 gcgtacgtaa atcgtcttaa caaccctggc ccggtacaca tgctaccggg cctacaacga
    41761 cagcgaaccg tgggccagag aagcggcaac acaggcgtag catacagttg gcaaatgtag
    41821 tacggggggc atatgacctg gtttattgac cggcgtctga acggcaaaaa caaaagcatg
    41881 gtgaatcgcc agcgtttttt acgccgttat aaagcgcaaa ttaaacagtc gatctccgag
    41941 gccattaata agcgttcggt gactgacgtc gacagcggcg agtctgtatc cattcccacg
    42001 gaagatatta gcgaaccgat gtttcatcag gggcgtggcg gtctgcgcca ccgcgtgcat
    42061 ccgggcaatg accatttcgt ccagaatgac cgaattgaac gtccccaggg tggcggcgga
    42121 ggttccggca gtggtcaggg tcaggccagc caggatggtg aaggtcaaga tgaatttgtc
    42181 tttcagattt cgaaagatga gtatcttgat ctgctctttg aagatctggc cttaccgaat
    42241 ctgaaacaaa accaacaacg ccagctgacc gaatataaaa cgcatcgtgc gggttatacc
    42301 gctaacggcg ttccggccaa tatcagtgtt gtgcgttcat tgcagaactc actggcacga
    42361 cgcacagcca tgacggcggg caagcggcgg gaacttcatg cgctggaaga gaatttggcc
    42421 atcatcagca atagtgaacc tgcgcaactg ctggaagagg aacgtctgcg caaagaaatt
    42481 gcagaattac gtgccaaaat tgaacgcgtc cctttcattg acaccttcga tttacgttac
    42541 aagaactacg agaagcggcc cgatccctcc agccaggcag tgatgttttg cctgatggac
    42601 gtttccggtt caatggatca gtccactaaa gatatggcta agcgttttta tattctgctg
    42661 tatctgttcc tcagcagaac atataagaac gtggaagtcg tatacatccg ccatcatacc
    42721 caggcgaaag aagtcgatga acatgagttt ttctactcgc aggaaactgg cggcaccatt
    42781 gtttccagcg ccctgaaact gatggatgag gtagtgaaag agcgttataa cccggcacag
    42841 tggaatattt acgcggcaca agcatcggac ggcgataact gggccgatga ctctccgctt
    42901 tgccatgaaa tcctggcgaa aaaattatta cccgtcgttc gttattacag ctatatcgaa
    42961 attacccgtc gcgctcatca gacactgtgg cgagaatatg agcatctgca atctactttc
    43021 gacaactttg cgatgcagca tatccgcgac caggatgata tttatccggt gttccgtgaa
    43081 ctgtttcata aacaaaatgc aacagctaaa gactaaaact atcagccggg tcattatcgc
    43141 ctggctgatt tttagctcac tgtaaattat ctcatttatt acatacatta gcttacaatc
    43201 gctttaaata tgacagcata acctttacac aatttagttc cagaaaacaa tcattcggaa
    43261 aaatgattcg gtcaacacgt gtttccctgg ggttattctt taaatatttt ttatcgttaa
    43321 cgaaaattga tcctggtcaa aactatatat ctctgccatc aataaaatcc agcactcaca
    43381 ttgctctcct ttttatggct tctatgggta cacaaaaatt aaaagctcaa ggctttttta
    43441 ttttcagctt attgctgacg ttaattttat tttgcattac taccttatat aacgaaaaca
    43501 cgaatgtaaa actcatccca cagatgaatt acctgatggt tgttgtggct ttgtttttcc
    43561 ttaacgccgt catttttctt ttcatgttaa tgaagtattt cactaacaaa caaattttac
    43621 caacactcat tttaagcctt gcatttttaa gtggccttat ctatttagtt gaaaccattg
    43681 taattatcca taaaccaatc aacggcagta cactgatcca gacaaagtcg aatgatgttt
    43741 ctattttcta tattttccgc caactcagtt tcatttgttt aacctcgctg gcgctctttt
    43801 gttatgggaa agacaacatc cttgacaaca ataagaaaaa aactggcatt ctgttactgg
    43861 cgctgattcc ttttttagtt tttccccttc tggcacacaa tctgagcagt tataacgctg
    43921 actattcttt gtatgtcatc gattactgtc cggacaacca tactgcgacc tggggaatca
    43981 actatacaaa aatattggtt tgtctgtggg catttttact gttctttatt atcatgcgta
    44041 cacgattagc cagcgaacta tggccgttaa tagcattatt atgtctggca tcgctatgct
    44101 gcaacttact tctactaact ctggatgagt ataattacac catctggtat atcagtcgcg
    44161 ggattgaagt ctccagtaaa ctgtttgttg tgtcttttct gatttataac atttttcagg
    44221 aactgcaact ctccagcaaa ctggcagttc atgatgtgct gaccaatatt tataatcggc
    44281 gctacttttt caacagcgta gagtcattat tgtcgcgacc tgttgttaag gacttctgtg
    44341 tcatgctggt tgatattaat cagttcaaac gcatcaatgc ccaatgggga catcgtgtgg
    44401 gtgataaagt gctggtttca attgtcgata ttatccagca aagcatccgc cccgatgata
    44461 ttttagcgcg actggagggt gaggtgtttg gcttgctatt taccgaactc aatagtgccc
    44521 aggcaaaaat cattgcggaa cgtatgcgta aaaatgtcga actcctgacc ggctttagta
    44581 acagatatga tgttcctgag caaatgacca tcagtattgg cacggttttt tcaacgggtg
    44641 acacacgtaa tatctcgctt gtcatgacgg aagcagataa agccttacgc gaagcgaaaa
    44701 gcgagggggg caacaaagtg attattcatc atatttaagt gcaaaaatat tcagagccat
    44761 gctttttgcg tggcttttgc atacaattta ttactattac ccctaaattt cccctcccca
    44821 gcggcgcaga gatgagtata attagcgccc ctgtgccagg ccgcaatcga actttatctg
    44881 gttttctcgt ttcactaacc gaaggagtgc catttatcat gaaattgcac catagaatgc
    44941 tccggcattt tatcgccgca agtgtcattg tgctgacatc ttccttcctt atttttgaac
    45001 ttgtcgccag tgacagagca atgagtgcct atctgcgcta tatcgtgcag agagcagact
    45061 cctcctttct ttatgataag tatcagaatc agagtattgc cgcgcatgtg atgcgcgctc
    45121 tcgctgctga gcagtcggaa gtgtcgccag aacagcggcg caccatctgc gaggcttttg
    45181 agtctgcaaa taatacccat ggcttaaacc tgactgccca taaataccct ggcttacgcg
    45241 gcacactaca aaccgcatcc actgactgcg acacaattgt ggaagctgca gcactattac
    45301 ccgcttttga tcaggcagtg gaaggcaacc gccaccagga tgattacggt tcaggtcttg
    45361 ggatggccga agagaaattt cactattatc tcgatctcaa tgaccgctat gtctattttt
    45421 atgagccggt taatgttgag tactttgcga tgaataactg gtctttcctg cagtcaggaa
    45481 gtattggcat cgatcgcaaa gatattgaaa aggtatttac cgggcgtacc gttttatcga
    45541 gcatttacca ggatcagcgt actaaacaga acgtgatgag tttgctgacg ccggtatatg
    45601 tcgcagggca gctaaaaggg attgtgctgc tggatattaa caaaaacaat ctgcggaata
    45661 tcttttacac tcatgaccgc cctctcctct ggcgttttct caatgtcacg ctaaccgata
    45721 ccgattcggg gcgcgacatt atcatcaacc agagcgaaga taatctgttc cagtatgtca
    45781 gttacgtcca tgacttaccg ggcggcattc gtgtctcgtt atccattgat attctttact
    45841 ttatcacgtc ttcgtggaaa agcgttctgt tctggatttt gacggcgtta attttgctga
    45901 atatggtgcg gatgcacttc cgtttatacc aaaatgtgtc gcgagaaaat attagtgatg
    45961 cgatgacagg actgtataat cgcaaaattt taacccctga actggagcag cggttgcaaa
    46021 aactggtgca atccggttct tcggtgatgt ttattgcaat tgacatggac aagttaaagc
    46081 aaataaatga caccctcggt catcaggagg gggatttagc gattacgtta ttagctcagg
    46141 cgattaaaca gtcgattcgt aaaagtgatt atgccatccg actcggtggc gatgaattct
    46201 gcatcattct agtcgattcg acgccgcaaa ttgcagcaca actgcctgaa cgtatcgaaa
    46261 aacgtctgca acatatcgcg ccgcagaaag agatcggctt ctcttccggt atttacgcga
    46321 tgaaagaaaa cgatacgtta catgatgcgt ataaagcttc cgatgagcgt ttatatgtca
    46381 ataagcagaa caaaaacagc cgttcatgat aaccttctgt ggttgtttgc ttgtaatctc
    46441 aggagcatga aatgactgaa atggctaaag gaagcatgac gcatcagcgg ttaatcgctt
    46501 tattatcaca agaaggtgct gacttccgcg tcgttaccca tgaagcggta ggtaaatgtg
    46561 aagcggtgtc agaaattcgt ggcaccgctt taggccaggg ggctaaggca ctggtatgta
    46621 aagtcaaagg caatggtgta aatcaacatg ttctggcgat cctcgctgcc gatcaacagg
    46681 ccgatctgag ccaactcgcc agccatatag gcggattacg cgcttcgttg gccagcccgg
    46741 cggaagtcga tgaactcaca ggctgtgtct tcggcgcgat cccccctttc agcttccatc
    46801 caaaactcaa actggttgcc gatcccctac tcttcgaacg atttgatgaa atcgccttca
    46861 atgcaggcat gctggataaa tccgttattt tgaaaaccgc ggattatttg cgcattgcgc
    46921 aaccagaact ggtcaatttc cgccgcactg cgtaactagc ctgtccgttc gataagaaga
    46981 acggacaaaa ccagcacaac agcaatggca aaaaacgatg acgtgataat cagtgtctcg
    47041 acgaacattt gatcgttcat agtatgcccc cgggagatag ctgttttcac gttattgtta
    47101 gcgtgcacaa attgcagttt gatgacagtt cgcctgattt tttatgcaaa aaacgtaaag
    47161 attttctact tccttcctgc agcaagcgta aagtaagcag gcttattatt ttttggcaag
    47221 gaaaccacga tgtttgatgt cactttgctg atcctgctcg ggttagctgc gctgggcttt
    47281 atcagtcata acaccactgt cgccgtttca attctggtgt taatcattgt ccgcgtcaca
    47341 ccgttaagca cctttttccc gtggattgaa aaacaggggc taagtatcgg gataatcatt
    47401 ctgacgattg gcgttatggc ccccatcgcc agcgggacgc taccgccctc aaccctgatt
    47461 cactcgtttc tgaactggaa atcgctggtg gcaattgcag taggcgttat tgtctcctgg
    47521 ctaggtgggc gcggcgtgac gttaatgggc agccagccgc aactggtcgc cgggctgctg
    47581 gtgggcactg ttttgggcgt ggcgctgttt cgcggcgtac cggttggccc gcttattgcc
    47641 gcgggtctgg tttcgctgat tgtggggaaa cagtagttaa tcccgcaata tatcggcctg
    47701 gcgtttgccc caggcctttc ttaaacatag taataaaggc cgttgttgaa tcatacccca
    47761 gcgtatgagc aactttctgt acagtatcgc ctttcactaa tccctgaagc gccataatca
    47821 gctgtaactg ctgacgccac tggcgaaaac tcaaccccgt ctctttcact atcagtcggg
    47881 caagattgcg ttcactcatc gcaaaaaagc gagcccattg ccccaatgac ccccactcga
    47941 cgggctcttt cgccatcatc tccaccatcg cgcgaatttt aggatgagaa gaaacgggta
    48001 ggtgcagctg ttgttgtggc tgctgaggga gttcatcaaa caggacctga attaatctgc
    48061 gggtcatcgg ctcagcacgc tgagtcgacg tcctgtttgc cagggttaaa attaattccc
    48121 ggcataaggg cgatattttc aatgtgcaac aggttgtcgg cattgtcact gcgctgggtt
    48181 cgataaacag aaaacagagt tcagcgttag ctgtcacttg attgctatgt tccactccgc
    48241 ccggtatcca aaccgcatat tgcggtggca ccatccacaa agcattttcc accgtacagg
    48301 taattgcacc atgtagcgcc agtatcagct gtcctttgcg gtgctgatgt acaggactgg
    48361 ttaactcatc cgttccggcg tgaatacaaa atgcgaccgc ggcttcatgg tgtcggtcgg
    48421 gttcatagcc attgagattc aacctgtgca tcattttgtc cgaatttagc gataatttgt
    48481 cattttagct tgattcaaca taacaataaa aacggtaagg tacagcctcg tttgtaacaa
    48541 tgagaagcat atgacctgtt caacttcatt aagcggcaaa aacaggattg tccttatcgc
    48601 tggcatttta atgattgcca caacattacg cgtcaccttt accggcgcag caccgttact
    48661 ggatacgatt cgttccgctt actcgctgac gacggcgcaa accggcttat tgaccaccct
    48721 gccattatta gcctttgcgc taatctcacc tctggctgcc ccggtggcgc gacgttttgg
    48781 tatggaacgt agcctgtttg ccgcgttact tttgatctgt gctggcatcg caattcgctc
    48841 tctcccttcg ccttacttct tatttggcgg tacagcggtc attggcggtg ggattgcatt
    48901 aggcaatgtc ttactgccag gattaattaa acgcgatttc cctcattccg tcgccagact
    48961 taccggcgca tattccctga caatgggagc tgcagcggca ctgggatcgg ctatggtcgt
    49021 gccgctggct ttgaacggtt ttggctggca aggcgcgttg ctcatgctga tgtcttttcc
    49081 tctgctggct ctttttttat ggctgccaca gtggcgaagt caacaacatg caaatttgag
    49141 tacctcacgc gccttacata ctcggggtat ctggcgttcg ccgcttgcct ggcaggtaac
    49201 attgtttctt gggatcaact cactggtcta ttacgtgatt attggctggc ttccggcgat
    49261 cctcatcagc cacggctata gcgaagcaca ggcgggttca ctgcatggtt tgctgcaact
    49321 agccacagca gctcccggtt tgctgatccc acttttctta catcatgtga aagatcagcg
    49381 tggtgttgca gcgttcgttg ccttgatgtg cgcagtgggc gcggttgggc tctgctttat
    49441 gccagcgcac gcgatcacct ggactctgct tttcggtttt ggttccggcg caacaatgat
    49501 actggggttg acgttcattg gtcttcgggc aagttctgcg catcaggcgg cggcactctc
    49561 ggggatggca caatccgtcg ggtatttgct ggcagcctgt gggccgccgc tgatgggtaa
    49621 aatacacgat actaacggtg actggtccat accattgatg ggtgttgcca tactttcact
    49681 actgatggcg attttcggac tttgcgccgg gagagacaaa gaaattcgct aatatccggt
    49741 gctatagtga cgtgaccaat catgcgtgga agggagaaca aacacgatga atattcagtg
    49801 caaacgcgtt tatgatccgg cagaacagag cgatggttat cgcgtactgg tcgaccgcct
    49861 ctggccgcgt ggtatcaaaa aaaccgattt agcccttgat gagtgggata aagaaatcac
    49921 gccgtcaacg gaactgcgca aagcctttca cggcgaagtc gtcgattttg caacctttcg
    49981 cgagcaatat cttgcagaac tggcgcaaca tgagcaagaa ggaaagcggc tggcggacat
    50041 cgccaaaaaa cagccgctga ccctgctcta ctcagcaaaa aacaccacgc agaaccatgc
    50101 gctggtgctg gccgactggc tacgtagctt gtgattttag tacagcatcc ggcggttatt
    50161 tttcaccagc cggatggtcc cgtcgccaca atgcccattc atcaatcgtt tcaccgcccg
    50221 gtaatttgca attgttgctg accccttgcg ctgtctgcac tggaatgagc gtcccgccct
    50281 tctgctggca atagaccgac gccggatttg ccataccaat ctgcggcggt ttgggtgctt
    50341 ctggctgaga aggggttgaa caaccagcca ggaccagcaa gcaaggcaga acaaaactga
    50401 taattttcat ttattgatct cacatattta tccagactta gagtatcgcg gtatcgtttt
    50461 gttttgcagc actattttta ttacattcac tcaaatcata ttacgccttg tttcatcttc
    50521 gttgatgatg ttttatcatg cctgcaaaga ttaaataatc agcatttacc cgccgtatcc
    50581 tggagttgtt tcgtgtcaga tcagattatc gcccgcgtct cgcaatccct tgccaaagaa
    50641 cagtcactgg aaagtctggt ccgacagctt ctggagatgc tggaaatggt cactgatatg
    50701 gaatcaacct acctgaccaa agtggatgtc gaagcgcgcc tgcagcatat tatgtttgcc
    50761 cgtaacagcc agaaaatgca catcccggag aattttaccg tctcgtggga ttactcgtta
    50821 tgcaaacgcg ccattgatga aaactgcttt ttcagcgatg aagtccctga ccgttggggc
    50881 gactgtattg cggcacgcaa tcttggcatc accacatttc tgagcacgcc aattcactta
    50941 ccggatggat cattctatgg cacgctttgc gccgccagta gtgagaagcg ccagtggagt
    51001 gaacgcgcgg aacaggtttt acagttattc gccggactga ttgcacaata tattcaaaaa
    51061 gaggcactgg ttgaacagct gcgcgaagcc aatgctgcgc tgattgcgca atcgtatacc
    51121 gactcgttaa ccgggctacc gaatcggcgg gcgatttttg aaaacctgac gacgctgttt
    51181 tctctcgccc gacatcttaa ccataagata atgatcgcgt ttatcgatct ggataacttc
    51241 aaattaatca acgatcgttt tggtcataat agtggtgatt tgttcctcat tcaggttggc
    51301 gagcgcctta atacgctcca gcaaaatggc gaagttattg gtcgtctcgg cggtgatgag
    51361 tttttggttg tttcactgaa caacgagaat gcggatattc gtctctgcga gaacgtattc
    51421 agcagcaaat atgcggagaa tatcacttag gtgatgttga tttgtattat cccggtgcca
    51481 gtcttggcat agtagaagtc gatcctgaaa cgaccgatgc agacagtgcc ctgcatgctg
    51541 ccgatatcgc gatgtatcag gagaaaaaac acaaacagaa aacacctttt gtcgcgcatc
    51601 cagcgctaca ttcctgaggc gtattcacat ccttttgatt ggtgataaca tgcgaatcgg
    51661 tattattttt ccggttgtaa tctttattac agcggtcgta tttttagcat ggttttttat
    51721 tggcggctat gctgccccgg gagcataaag atgaaaaaaa caacgattat tatgatgggt
    51781 gtggcgatta ttgtcgtact cggcactgag ctgggatggt ggtaacgtca catctagaaa
    51841 atagcaaagg ctgcctgtgt gcagcctttg tgcaatttac gcgttaactt ttaatctttc
    51901 tgtagataaa cagcacgaca atcgcgccaa taacggcaac cacgaagctg ccaaaattga
    51961 agccatcgac tttaccaaag ccaaacagcg tgctgatcca tccgccgact acggcaccga
    52021 ctatccccag caggatagtc ataaagaatc caccaccatc tttacctggc atgatccact
    52081 tcgccagaat accggcaata agcccaaaaa taatccatga cagaatgccc attgtttcct
    52141 cacttatctg ttttgcatta gcgggttagt cgctgataaa aagcatagca caacatcggg
    52201 agggcaagat ttgtgacgag catcacggag gatttttttg cgatggcgca gaaattgcgc
    52261 catcaacgat cagtgataat taccaaccac aaacatcatg ttcgttttcc gtgtcataag
    52321 aacgcacggt attcaccaga tcttttatca cttcagccgc cacttctggc accagcaaag
    52381 tcatcggcgt ctctgtttca taatcgacag aaacgccatt gctgttattg gtgacggtca
    52441 cggtatacgt tgctttgccc atgattcatt tcccgttatg aatgactttc cgttgttgcg
    52501 caccttccat caggacttca ggggcaacga agaagtcaat gttgaaataa gtatcgtcag
    52561 tcatggcttc aatgttgtgc cacttttctg gagggaacac cgcaaactgc cccgcttcga
    52621 taaggatcac ctgatcaggc tctgcactgt gttcatcagg gtagccgaga tatttgaccg
    52681 ccccatgcat aacggaaagg cgtgggtaaa ctcccgggcg cgttccttta tcaagatgac
    52741 gttcgaatat tccggcaggc gcagtttgtt tattccagaa aggcgttgag cgcgtatgaa
    52801 tataattctg tgggatttgc agcatccttt tccctccttc ggtgaatgcg ctgaaaatgg
    52861 tttattccag ccgtttcagg gtacgcctga taatttgcat tttaaatacc atttattggt
    52921 tactttttag caccatatca gagaagaatc agggaggatt acagatggga agcccgcgac
    52981 aacgcgggct gaaagcatca ggattgcagc gtcgccagtc gggcggcgaa acccacgaac
    53041 atcaaaccaa tcagtgagtt gcccacttta gccagtttct ttttggtacg tatgtactgt
    53101 gtgacaaaag caccagaaat aatcaggaag ctcaaatagc agaaactcac cagttccagc
    53161 gtcgtcgcca gaataaagaa tgaaattccc gtatgtgggg cattaacatc gataaactgt
    53221 acgaaaaacg acacatagaa caaaatggct ttcggattag tcaggctcaa aattaacgcg
    53281 cgtttaaaaa tggcaccgta ttgaggctca tcggatttgg cctcgttatt tttacccttc
    53341 agggtcgcgt agagaatttt actccccaga tagagcaaat aaaacgcacc aagataacgt
    53401 acaatgttga ataatatcgg ggtggtctta attaatgtcg ccactccagc ccatgccaga
    53461 aacatcaata ccgcatcgcc aataaataca ccgcaggccg caagataacc gcctttcata
    53521 ccgctactga cgctattttt gagtacaaac agggtatttg gccctggcac caacacaata
    53581 aaaatggccc caaccagata ggtccagtaa ttcagaaccc cgtattcagc gaacacatta
    53641 acctctttaa ttatctttcg atcatgcgcg attaaaggtg aatatgctaa ccaatctgta
    53701 gcggcttaga aaggagaaaa tcaggtttta acccgatatc aacccgataa ttgaatcatt
    53761 aatcaggcat gctccagtga aaaattcggg tagtgctcgg caaaatactg gcgtaaaaat
    53821 tctaccgtta tccgcacttt cgctgacgtc gccagccttg aaacataaac ggcccagaca
    53881 ttcgctggct ggtaatattc cggtagcaac tgcactaaat gaccactggc aatattttcg
    53941 ctaacatccc accaggagcg cagagcaatc ccctgcccat caagacacca ctgatggaca
    54001 atttctccat gattagacga cagtgggccg gtaactttaa tcgcatggtg gccttcttta
    54061 ttgcgtaatt gccagacacc aaaaggatga tcgcgctctt taatgaccag gcacggcaat
    54121 gcagaaagat cggttaaatg tttcggcgcg ccatgttgcg caataaattc cggtgacgcg
    54181 cagagaatgc gataattggt cgccagtttg cgggcgatta aattaggggc gatatcatcg
    54241 ccaatgcgaa tatcgagatc gacaccttca ttgaccaaat cgaccaaccg gtcttccaca
    54301 tcaaaacgta attccagttg cggatacgcc ttcgccagcg ctgataacgc cggggccacc
    54361 acctgtcgtc caaaaccaaa gctgctgata atacgcagca tcccctgcgg cacctgacgc
    54421 acgtcagaaa gttcgtccat catttgaccg acatcctgca aaatccgctg cgcccattca
    54481 taaatccgct ctccttcttc ggtaatggtg acacggcggg tggtgcggtg taacaacacc
    54541 acgtttagcg tttgctccag caaggcgatg cgcttgctga cgaacgctgg agaaacgccc
    54601 agttcttccg ccacggcggc aaaaccagcc cggcgagcca ccagcataaa gacgcgcaaa
    54661 tcgttcagca gcggtaaatt attcatgatt cgtgttttat gtttcaccag ttacagggat
    54721 taattccttt tcagtcaatt ataggatggt gatgttgtca attttgatgg tcaggaagtg
    54781 agaacccaat gatgaaaacg atgcgtattg ctgcgatccc gggagacggg attggcaaag
    54841 aagtccttcc tgaagggatt cgcgtgttac aggctgccgc tgagcgctgg ggcttcgcct
    54901 tgagttttga gcaaatggag tgggcgagct gcgagtatta cagccatcac ggtaaaatga
    54961 tgccggacga ctggcatgag caacttagcc gtttcgacgc catctatttt ggtgccgtcg
    55021 gctggccgga caccgttccg gaccatattt cgttgtgggg ttcgctgctg aaatttcgtc
    55081 gtgaattcga ccagtacgtc aacctgcgcc cggttcgtct ctttcctggc gttccctgcc
    55141 cgctggcggg aaaacagcct ggcgacatcg atttttacgt ggtcagggaa aacaccgaag
    55201 gcgaatattc ctcgctcggc ggcagagtga atgaaggtac agagcatgaa gtcgtcattc
    55261 aggaatcggt atttacgcgt cgtggtgtcg atcgcatttt gcgttatgcc ttcgaacttg
    55321 cgcaaagccg cccacgtaag acgctaactt ctgccactaa atcgaacggt ttagccatca
    55381 gcatgccgta ctgggatgag cgagtggaag caatggccga gaattacccg gagatctgct
    55441 gggacaagca gcatattgat attctctgcg cgcgttttgt gatgcagccg gaacgcttcg
    55501 atgtggtagt ggcgtccaat ttgtttggcg atatcctttc cgatcttggc ccggcctgca
    55561 ccggcaccat tggcattgcc ccatccgcca acctgaatcc ggaacgcact ttcccgtcgc
    55621 tcttcgagcc agtccacggt tccgcgccgg atatctacgg gaaaaacatt gctaacccta
    55681 tcgccacaat ttgggccggt gcaatgatgc tcgattttct cggcaatggc gatgagcgtt
    55741 tccagcaagc gcataacggt attctggccg cgattgaaga agtgattgct cacgggccga
    55801 aaacacctga tatgaaaggc aatgccacca cgccacaggt tgccgacgcg atttgcaaaa
    55861 ttattttgcg ttaaggtcaa accagtttat ttgaaccgcg tcactgacgc ggttttttta
    55921 ttcgttcttt gcagttaata acctgcgtca tttcaccttt tattgtttcc gtttcgtgtt
    55981 ttatggcttt ccgtattctt aattgtttaa tttatgtaac atgtaaataa tcaatttgtt
    56041 aaacacaaca tgcaactcat taccctgcaa ataattacaa ctggcaaaag ccagagacgg
    56101 gcttttatgc ccaggagatg actcatgatg agcaatgtta agaaaaaaga tgtgccgctg
    56161 ataagcatca gcctggtggc cattcttttc atcgcagctg cattaagcct tttcccacaa
    56221 caatcggccg acgcggccaa cgcaatatac acttttgtta cgcgtacgtt aggttccgcc
    56281 gtacaggtat tggttttgct ggcaatggga ctggtgattt atttagccac cagtaaatac
    56341 ggcaatattc gtcttggaga aggaaaaccg gaatacagca cgctctcctg gctgtttatg
    56401 tttatttgtg ccggtttagg ttcttctacg ctttattggg gggttgctga atgggcctat
    56461 tattatcaaa cacctggatt aaatatcgca ccgcgttcac aacaggcact cgaatttagc
    56521 gtcccttact ccttcttcca ctggggcatc agcgcctggg caacttatac gctggcctca
    56581 ttaatcatgg cttatcactt tcatgtgcgg aaaaacaaag gtctgagcct ttccggcatt
    56641 attgccgcga ttaccggcgt tcgcccgcaa ggcccgtggg gaaaactggt cgatttgatg
    56701 ttcctgatcg ccactgtcgg tgcgctgacc atttcccttg ttgttaccgc agcaaccttt
    56761 actcgtgggc tttccgcgct gaccggttta cccgataact tcaccgtgca ggcatttgtg
    56821 atcctgcttt ccggcggcat tttttgccta agctcgtgga ttggtatcaa caacggtttg
    56881 caacgtctga gcaaaatggt tggctggggc gcgttcctgc tgccattact ggtgctgatt
    56941 gtcggtccaa ccgaatttat taccaacagc atcatcaatg ccatcggcct gaccacgcaa
    57001 aacttcctgc aaatgagctt attcaccgat ccgcttggcg atggttcatt tacccgcaac
    57061 tggaccgttt tctactggct gtggtggatt tcatacaccc ctggcgtagc aatgtttgtc
    57121 acacgcgttt cacgcggtcg taagattaaa gaagttatct ggggactgat cctcggcagc
    57181 accgtcggtt gctggttctt cttcggcgta atggaaagct atgccattca ccagtttatc
    57241 aatggcgtaa tcaacgtccc acaggtgctg gaaacactgg gcggcgagac agctgtgcag
    57301 caagttctga tgtcgttgcc agccggtaaa ttgttcctcg ccgcatacct gggcgtgatg
    57361 attattttcc ttgcctcgca tatggatgcg gtggcctaca ccatggcggc gaccagtacg
    57421 cgaaatctta aggaaggtga cgaccctgac cgtgctcttc gcctgttctg gtgcgtggtt
    57481 atcaccctga ttccactgtc tatcttattt actggcgcgt cgctggaaac catgaaaacc
    57541 actgtggtac ttacagccct gcccttcctc gttattttac tggtgaaagt cggtggcttt
    57601 attcgctggc tgaaacagga ttacgccgac attccggctc atcaagttga acattatctc
    57661 ccgcagacac cggttgaagc cccggaaaaa acgccagtgc tccctgcggg aaccgtattc
    57721 aaaggcgaca actgagcgcc atcatcctaa cgataaaggt aaccctatga gcaatctgag
    57781 ccctgacttt gtactacccg aaaatttttg cgctaacccg caagaggcgt ggaccattcc
    57841 tgcccgtttt tataccgatc agaacgcctt tgaacacgaa aaagagaacg tcttcgccaa
    57901 aagctggatt tgcgtcgctc acagcagcga actggcgaat gccaatgatt atgtgacgcg
    57961 tgagatcatt ggcgaaagca tcgtgctggt acgtggtcgt gataaggttt tgcgcgcgtt
    58021 ctataacgtt tgcccgcacc gtggtcatca gttattgagc ggcgaaggga aagcgaaaaa
    58081 tgtgattacc tgcccgtatc acgcctgggc attcaaactc gatggtaatc tggcccatgc
    58141 gcgtaactgc gaaaacgtcg ccaatttcga tagcgacaaa gcgcaactgg ttccggtgcg
    58201 tctggaagaa tatgccggat tcgtcttcat caacatggac cccaacgcca ccagcgtaga
    58261 agatcagtta cccggtctgg gggcgaaagt gctggaagcc tgcccggaag tccacgatct
    58321 gaaactggcg gcccgcttta ccacccgcac gcctgccaac tggaagaaca ttgtcgataa
    58381 ctatctcgag tgctatcact gtggtccggc gcatccaggt ttctccgact ccgtacaggt
    58441 tgatcgttac tggcacacca tgcacggtaa ctggacgctg cagtacggtt tcgccaaacc
    58501 gtccgaacag tctttcaaat tcgaagaggg cacggatgcg gcattccacg gtttctggct
    58561 gtggccgtgc acgatgctga acgtcacccc gatcaaaggg atgatgacgg tcatttatga
    58621 attcccggtg gattctgaaa ctaccctgca aaactacgat atttacttca ccaatgaaga
    58681 gttaaccgac gagcaaaaat cgctgattga gtggtatcgc gatgtgttcc gtccggaaga
    58741 tttacgtctg gttgaaagcg tacagaaagg gctgaaatcg cgtggctatc gtggccaggg
    58801 gcgcatcatg gccgacagta gcggtagcgg aatttccgaa catggtatcg cccacttcca
    58861 taatctgctg gcgcaggtgt ttaaggacta atgacatcgg cggcggtatt ttccgccgct
    58921 gggctgattt ttgatggagt acagcaatgt cagactatca aatgtttgaa gtacaggtga
    58981 gccaggttga accgcttacc gaacaggtga aacgcttcat gctggtggca accgatggca
    59041 aaccattacc tgcgtttacc ggaggaagtc acgtcattgt gcagatgagc gatggtgata
    59101 acgagtacag caatgcgtat tcactactga gttcgccgca tgacacctct tgttatcaga
    59161 ttgccgttcg actggaggaa aactcgcgcg gcggttcccg ctttttgcat cagcaggtaa
    59221 aagtgggcga tcggttaacg atttcaacgc ctaataacct gtttgcgcta attccctcgg
    59281 ccagaaagca tctgtttatc gcgggcggta ttggtatcac ccctttcctg tcacacctgg
    59341 cagagttgca atacagcgac gtcgactggc agttacatta ctgctcgcga aatccagaaa
    59401 gttgcgcatt tcgtgatgag ctagtccagc atccgcaggc tgagaaagtt cattttcatc
    59461 attcatcaac cggaacacga ctggaattag cgcgattatt ggcggatatc gaacctggca
    59521 cacatgttta tacctgcggc cccgaggcgc taattgaagc tgtaagaagt gaagctgcgc
    59581 atctggacat cgccgccgat acgctgcact ttgagcaatt tgcgattgaa gacaaaaccg
    59641 gcgatgcatt taccctggtg cttgcccgtt ccggtaaaga gtttgtggtg ccggaagaga
    59701 tgactatttt gcaggttatt gaaaataata aagccgcgaa agtggaatgt ttatgtcgtg
    59761 aaggggtatg cggaacctgc gaaacggcaa tactggaagg tgaagctgac catcgggatc
    59821 agtattttag cgatgaagag cgtgccagcc agcaaagtat gttgatctgt tgttcgcgcg
    59881 cgaagggtaa acgcctggtg ttggatttgt agtttgccga agccggatgt ggcgctgagt
    59941 gcgcccagtc cggcttcgga agattttact gcggatattc ctgcaataaa ttgtgtaatg
    60001 cttccgccat cagctcacca cgccagccgg aaatcagctc cggcaaattg ttctgcggtt
    60061 tcagtttcca gtgccagttc agcagttggt tgatttgccg acgcgatgcc agcaattcgg
    60121 cgctgatctt gtgcgtttcg ctcacgtcag taatcagcga cttaatcgct ttaaacgctt
    60181 tacgataacc cggcatgtcc atcaggttaa gcatcggctg cggtaaggcc tcttccggca
    60241 atgtctgcgc tttttccacc agcgccagca gcgttttacc gtgaaagcgg atttcgctac
    60301 cggataagcc caggctgtcc agttcgccta aacttcccgg catataacgc gctaccgacc
    60361 acaaatgctc ttcgcgcacg acaaagttca ccgccagatc gcgctcccgc gccttgcgca
    60421 gtcgccagtc ggctaacagt tgcagacagg ccagttggcg tgtgcgtaac tgccaggcat
    60481 tggtgatatc acgccaggca tcttccggtg caacgacttc ctgacgacgc atttgcatca
    60541 ggcggcattc atccagcgcc gcaggtagcc agccggaggc ctccgtttct accataagct
    60601 ttgcggtgat cggtaacaga taccagacat ccgccgctgc gtattcacac tgacgttcgg
    60661 tcagcggtct ggccagccag tcggtgcgcg attcactctt gtccagcgta acgccggaat
    60721 actcttccac catggaagcg aaaccccatg atatcgggcg tccgcagaag gcagcaagga
    60781 tttgcgtgtc aatcaggggt tgtggtaatt cgccaaagac attgaggaac acttccagat
    60841 cttcactgcc tgcatggaga aattttgtga tggacggatc gcgcaggatc gctttcagcg
    60901 gtgaccagtc ggtgatcccg agaggatcaa ttagcgccag atgctcgcca tcgaaaagtt
    60961 gaattagacc cagttgcggg taataggtgc gcgtacgaac aaattcagta tccagggcta
    61021 tcgccggaaa ggcacggacg gcttcacaca aagaagccag cgcatcgtcc gtggtaatca
    61081 tttggtaatt caaattgtat tctctttagt gggcgtcaaa aaaaacgccg gattaaccgg
    61141 cgtctgacga ctgacttaac gctcaggctt tattgtccac tttgccgcgc gcttcgtcac
    61201 gtaattctcg tcgcaaaatt tttccgacgt tagatttcgg taactcatca cgaaactcca
    61261 ccaacttcgg tactttgtag cccgtgagct gacggcggca aaaagtcacc agtgactctt
    61321 cggtaagcga tggatctttt ttcactacga agattttcac cgcttcacca ctggagccgg
    61381 aaggtacgcc aacagccgcg acttcctgta cgccaggatg ctgcatgacg acatcttcaa
    61441 tctcgttggg atagacgtta aaaccggaaa ccagaatcat gtcttttttg cgatcgacaa
    61501 tacgcaggaa tccttcttca tccatcaccg cgatgtcgcc ggtgtgtaac cagccatttt
    61561 tgatgatttc atcggtagca tccggacgct gccagtaacc cagcatcacc tgcggccctt
    61621 tgacacaaag ctcacccggt tgacccggtg gtacttcgtt gtcgtcgtca tccaccagtt
    61681 tggcttccgt cgatggcacc ggcaaaccga tgctaccgct atgataatca atatcatacg
    61741 ggttaacgct gaccagcggc gcacactcgg tcaggccata accttccagc agatactgtc
    61801 cagtcagttt cacccaacgt tctgccacca cttgctgaac aggcatccct ccgcctgcgg
    61861 aaagatgcag actggagaaa tccagctgct ggaactcttt attgttcagc aacgcattga
    61921 acaaggtgtt aacgccagtg atagcggtaa acggatattt cgctaactct tttaccaacc
    61981 caggaatatc gcgcggatta gtgataagca ggttctgccc ccccagttcg ataaacagca
    62041 ggcagttaat ggtcagggca aaaatgtgat acagcggcag cgccgtcacc accagctctt
    62101 tgcccggatg caacagcgga ccataggtcg cgttaacctg ttccaggttc gccagcatat
    62161 tgcggtgagt cagcatcgcg cctttcgcca caccagtggt accgccggtg tattgcagga
    62221 aagctaaatc ttccggcacc agttcgggtt tgacgtactg catccggtag ccgttgtgca
    62281 gggcgctacg aaatgaaatg gcatctggca gatggtattt cggcaccaga cgcttgatgt
    62341 atttaacaac gaaattgact accgtgcctt ttgccgtaga tagctgatcg cccatacggg
    62401 tcagaattac gtgctgaacg gcggttttat caaccacttt ttccagagtg tgggcaaagt
    62461 tagacacgat aacaatcgcc gatgcgccgc tatcgttaag ctgatgctca agctcacgcg
    62521 gggtatacaa cgggttaacg tttacaacga tcatcccggc acgcaaaatg ccaaacagcg
    62581 ccaccggata ttgcaataaa ttaggcatca tcaacgcaac gcgatcgcct ttcttcagtc
    62641 ccaacccttg ttgcaaataa gcggcaaacg cgcgactgcg ttcttccagt ttgcggaagg
    62701 tcatcacctc ccccatattc acaaacgcgg gttgatcggc atagcgcgcg accgactgct
    62761 caaacatatc taccagagat tgataacggt cagggttgat ctccgtcgga acgtccgcgg
    62821 gataacggtt aagccaaacc ttcttcaatt cttcacctct aaaatgcgtg ttcgtcgtca
    62881 tcgcaacccc aaatgatata catgccgtta acataatatt aactcatcat accagcttga
    62941 taattaccca acgaaaaggt tgcgaagcgc gtcactattt atttttatct ttaccgtaag
    63001 aatgcagaaa cagcggacca gccgctgttt ctttttcttt aaaaacaagc aattattcag
    63061 ttacaacagt ttgtactctc gcggggccgg gattatacca gccccatcca ccgtagccat
    63121 agggccagcc acgaccgcca taaaaccatg gatcaatcgg ctgaggcggc ataatcacct
    63181 gctgggttaa atgccaacgt ttgtaacccg ttacctgcat caccataaat ttatagggcg
    63241 tgttgccgat tttgccgtca accgcaccgg tgattggccc aactaccgta accagttgtc
    63301 cacggaaatc taccgggtcc agaaaaccgt tcacatcggc ataaatgcgc ccgcgtgaag
    63361 gttcccccaa agttggtctg gctccgctgt ccagcggtac ggtagcaatt tccaggcggg
    63421 ttttcccttg ctggttttgt accgcaacca ctttgccacc aaagcgtgcc tcctgaccaa
    63481 cgtacagctg cggcgcactc atcacccgaa ctaaatcttg ttgcggcgtg ggactgctgc
    63541 ctttaatggc gtccggcaca gtgacacaac cgctcagcat tagcgcaaac gtacctgcca
    63601 gtatgccttt gataacgttc ttttgaaccg ccatgatgcg actccttttt ttctcagggc
    63661 atactcttaa gattcattct ttgcccggaa gtttcttcca tgcgacgttg ttacgtaaat
    63721 aaaccggttc ggcatgttct accgccaccg ttttaccctc ggcaaacatc tgacaagcaa
    63781 tcggcagcat atctcctgca gcggggagta acacttcgcc atcgcgcaaa accagcccgc
    63841 tctctttacc gagatccggc caggcttgcc agcccgtccc taccgtcacc cattcgccgg
    63901 aaagctgttg cattcgttca tgaacgagtt cgggtttgag tacggcttcg gtttcttcac
    63961 cgtgccagat accgttttca tcacgctgat attcggccca gtaaacttcg cccattcgcg
    64021 cgtcaatggc tgccagcacg cgtgttgcgc cgtttttgcg ccacgcccct tgcgccatcg
    64081 tcattagcgt ggagacgcca atcatcggta attccgcgcc aagcgccagc ccttgcgcga
    64141 tgccaatacc aatgcgcacg ccagtaaagc taccggggcc gcgcccgtaa gccagagcgt
    64201 taatatcagt cagggaagtt ccgctggtgg tcaggatatc ctgcaccatc ggtaagattc
    64261 gttgagtatg ttcacgaggg caaagctcaa aatgagcgtt gaccgtaccg tcgttccaca
    64321 gggcgacaga gcaggcctct gtcgcggtat cgatagccag aattcgcatg ggtcttcgtg
    64381 cttagatcaa taaaaaggcg cgcatcatac catactccat cacaaattac ctggaggatg
    64441 gtatcgcaag gaaacgaacc gcacgggcaa tgtcacgggt gcgcggcgct ggcggcagac
    64501 tggcgagaaa cgtcgcgccg taagggcgca tcaccagccg attatcgcaa atcaccaaaa
    64561 cccctcgatc gtcggcgtcg cgaatcaatc gccctactcc ctgcttgaga gtaatgaccg
    64621 catccggcag ctgtacttca tcgaacgggt cgcctccgcg caaacgacaa tcttccatgc
    64681 gcgcttttaa tagtggatca tccggtgagg taaacggcaa cttgtcgata atcaccagcg
    64741 acaatgtatc gccacgcaca tccacccctt cccagaagct gctggttgcc acaagaagcg
    64801 cgttaccggc gctgacaaat tgctgcaaca gttgcccttt gctggtttcc ccctgcaaca
    64861 aaacgggaag cgtcatggta gcgcggaact gctcagccag atcgcgcatc atggcgtgcg
    64921 aggtacaaag cataaaacaa cgaccgttgt tagcttcgat gatcggtcgc agcattgccg
    64981 ccagttgccg tgcggaaccc ggttgattag tttgcggcag gttgcgcggc acacagagta
    65041 acgcctggcg gctgtaatca aaggggctgg gtaacagcaa cgactccgcc tgttcgatgc
    65101 caagccgcga ggtgaaatga tgcagatcgt cgttcaccga cagcgttgcc gaggtaaaaa
    65161 tccagctacc tggcttttgc gccattaact ctttaaattt atccgccacg ctgagcggcg
    65221 tgagtgccag agtaaaatgg cgcgaagtgc attcgtacca gtagctgtag cctggctgat
    65281 tgatctcttt tagccgtttc agccgcgtac gatacaacgt ggcgcgctca aatgccgcat
    65341 ccagcaaggc ggagcgcccc agcgacagtt tcgccacgtc ataacaaagt tccagggtgt
    65401 catcgagcag taaaaatgct cgctgaattt gcggattagc taacagttcg cgcaggttgc
    65461 cacgataacc aggctcaccg agttgcagac gaaaatcctg cgcgctctgg gcaaggcggt
    65521 cggcgcactt ttgtaactgc tgagtatctt ttaattcggt gcgataggcg atggtgatgt
    65581 cttttgccag gtcgagcagt tgtcgactgg agagtgactg accaaaatac tggctggcaa
    65641 tgtcgggcag ttggtgggct tcgtcgaaga tcatcacgtc agcttccggg atcagttcgc
    65701 caaatccact ctctttcacc accatatccg ccagaaagag atgatggttt accaccacca
    65761 catcggcgtc catcgctttt ttgcgtgctt tgaccacaaa gcaatcttta tacatcgggc
    65821 agtcgctgcc aagacagttg tcgttggtgc tggtgaccag cggccacgcc tgtgaatctt
    65881 ccgccacgct gacgcaggtg ctgatatcac catcgactgt ttgattagac caggagcgca
    65941 gcaggatcac atcgcttaag atttgtaccg gcagatcgcc gcccgctagc gcctgttgtt
    66001 cgagacgttc gaggcagagg tagtttgagc gccctttcag cagcgccacg ttgcccgtat
    66061 atttcaatgc ctttgagact gttggcaaat cgcggctgta gagctgatcc tgcaacgctt
    66121 ttgaaccggt cgagataatg actttctttt tcgcccgcag cgcaggggcc aggtaagcgt
    66181 aggttttgcc cgtaccggtt ccggcttcca ccaccagcgg ctggcctttt tctatcgcct
    66241 gggtgacggc taccgccatc tgtcgctgtg gttctcgcgg cttaaagccc ggtatcgctt
    66301 tcgccaactg accgtctggt gcaaaatcgt ccgtcacact accccctgtt gatttgaaca
    66361 gggattatgt caggatgagg gcgctttcgc cagttgaagt ggtgacggcg acctcacatt
    66421 gtggcagtct ttgcagcacg aaaatggaag gataatgagg acaaaatgat gactatcgtt
    66481 cgtatcgatg ctgaagcccg ctggtctgat gtagtaatcc acaacaacac gctctactac
    66541 actggtgtac cggaaaacct cgacgccgat gcctttgagc aaaccgccaa cacgctggcg
    66601 cagattgacg ccgtgctgga aaaacagggc agcaataaat cgagcattct ggatgccacc
    66661 attttcctgg ccgataaaaa cgacttcgcg gcgatgaata aagcgtggga tgcctgggtt
    66721 gtcgcgggtc atgcgccggt gcgctgcacg gtacaagcgg gtttgatgaa cccgaagtat
    66781 aaagttgaaa ttaagattgt ggctgcggtg taagctttat cgaagcaaaa taagtcagac
    66841 gataatttat cgataatact ggtcggtttt acataaatcg accaga
//
LOCUS       DACBVI010000006        65470 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.006, whole genome shotgun sequence.
ACCESSION   DACBVI010000006 DACBVI010000000
VERSION     DACBVI010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.006"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            81..644
                     /gene="srlA"
                     /locus_tag="IU325_02025"
                     /gene_synonym="gutA"
     CDS             81..644
                     /gene="srlA"
                     /locus_tag="IU325_02025"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP5807813.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            641..1600
                     /gene="srlE"
                     /locus_tag="IU325_02030"
                     /gene_synonym="gutE"
     CDS             641..1600
                     /gene="srlE"
                     /locus_tag="IU325_02030"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP5807814.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            1611..1982
                     /gene="srlB"
                     /locus_tag="IU325_02035"
                     /gene_synonym="gutB"
     CDS             1611..1982
                     /gene="srlB"
                     /locus_tag="IU325_02035"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP5807815.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            1986..2765
                     /gene="srlD"
                     /locus_tag="IU325_02040"
     CDS             1986..2765
                     /gene="srlD"
                     /locus_tag="IU325_02040"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HAP5807816.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            2871..3230
                     /gene="gutM"
                     /locus_tag="IU325_02045"
     CDS             2871..3230
                     /gene="gutM"
                     /locus_tag="IU325_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HAP5807817.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            3297..4070
                     /gene="srlR"
                     /locus_tag="IU325_02050"
                     /gene_synonym="gutR"
     CDS             3297..4070
                     /gene="srlR"
                     /locus_tag="IU325_02050"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HAP5807818.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEKKGIDVIITGESNE"
     gene            4063..5028
                     /gene="gutQ"
                     /locus_tag="IU325_02055"
     CDS             4063..5028
                     /gene="gutQ"
                     /locus_tag="IU325_02055"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HAP5807819.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(5025..6539)
                     /gene="norR"
                     /locus_tag="IU325_02060"
     CDS             complement(5025..6539)
                     /gene="norR"
                     /locus_tag="IU325_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HAP5807820.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYNFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            6726..8165
                     /gene="norV"
                     /locus_tag="IU325_02065"
     CDS             6726..8165
                     /gene="norV"
                     /locus_tag="IU325_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HAP5807821.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSANTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            8162..9295
                     /gene="norW"
                     /locus_tag="IU325_02070"
     CDS             8162..9295
                     /gene="norW"
                     /locus_tag="IU325_02070"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HAP5807822.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEIGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCTE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(9373..11613)
                     /gene="hypF"
                     /locus_tag="IU325_02075"
     CDS             complement(9373..11613)
                     /gene="hypF"
                     /locus_tag="IU325_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HAP5807823.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVAEGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAASLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TTSVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRTRLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RILHAA"
     gene            complement(11766..12293)
                     /gene="hydN"
                     /locus_tag="IU325_02080"
     CDS             complement(11766..12293)
                     /gene="hydN"
                     /locus_tag="IU325_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HAP5807824.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(12442..13452)
                     /locus_tag="IU325_02085"
     CDS             complement(12442..13452)
                     /locus_tag="IU325_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP5807825.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHSIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRGSLIAL
                     SQ"
     gene            13712..15169
                     /gene="ascF"
                     /locus_tag="IU325_02090"
     CDS             13712..15169
                     /gene="ascF"
                     /locus_tag="IU325_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HAP5807826.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTISGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDSITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEATAEARKHQSAQPTVAKEVSLN"
     gene            15178..16602
                     /gene="ascB"
                     /locus_tag="IU325_02095"
     CDS             15178..16602
                     /gene="ascB"
                     /locus_tag="IU325_02095"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP5807827.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAYREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     ELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARICFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLIASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDEFAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDVGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(16681..17100)
                     /locus_tag="IU325_02100"
     CDS             complement(16681..17100)
                     /locus_tag="IU325_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP5807828.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(17097..17408)
                     /locus_tag="IU325_02105"
     CDS             complement(17097..17408)
                     /locus_tag="IU325_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase HigB"
                     /protein_id="HAP5807829.1"
                     /translation="MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(17575..18045)
                     /gene="hycI"
                     /locus_tag="IU325_02110"
     CDS             complement(17575..18045)
                     /gene="hycI"
                     /locus_tag="IU325_02110"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HAP5807830.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(18038..18448)
                     /gene="hycH"
                     /locus_tag="IU325_02115"
     CDS             complement(18038..18448)
                     /gene="hycH"
                     /locus_tag="IU325_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase maturation protein HycH"
                     /protein_id="HAP5807831.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(18445..19212)
                     /gene="hycG"
                     /locus_tag="IU325_02120"
     CDS             complement(18445..19212)
                     /gene="hycG"
                     /locus_tag="IU325_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HAP5807832.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(19212..19754)
                     /gene="hycF"
                     /locus_tag="IU325_02125"
     CDS             complement(19212..19754)
                     /gene="hycF"
                     /locus_tag="IU325_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HAP5807833.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(19764..21473)
                     /gene="hycE"
                     /locus_tag="IU325_02130"
     CDS             complement(19764..21473)
                     /gene="hycE"
                     /locus_tag="IU325_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="formate hydrogenlyase subunit 5; HycBCDEFG is part
                     of the formate hydrogenlyase system which is involved in
                     the cleaving of formate to dihydrogen and carbon dioxide;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HAP5807834.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWITVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(21491..22414)
                     /gene="hycD"
                     /locus_tag="IU325_02135"
     CDS             complement(21491..22414)
                     /gene="hycD"
                     /locus_tag="IU325_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HAP5807835.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(22417..24243)
                     /gene="hycC"
                     /locus_tag="IU325_02140"
     CDS             complement(22417..24243)
                     /gene="hycC"
                     /locus_tag="IU325_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HAP5807836.1"
                     /translation="MSAISLINSGVAWFAAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATSAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(24240..24851)
                     /locus_tag="IU325_02145"
     CDS             complement(24240..24851)
                     /locus_tag="IU325_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAP5807837.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(24976..25437)
                     /gene="hycA"
                     /locus_tag="IU325_02150"
     CDS             complement(24976..25437)
                     /gene="hycA"
                     /locus_tag="IU325_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HAP5807838.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            25649..25999
                     /gene="hypA"
                     /locus_tag="IU325_02155"
     CDS             25649..25999
                     /gene="hypA"
                     /locus_tag="IU325_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAP5807839.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            26003..26875
                     /gene="hypB"
                     /locus_tag="IU325_02160"
     CDS             26003..26875
                     /gene="hypB"
                     /locus_tag="IU325_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HAP5807840.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            26866..27138
                     /gene="hypC"
                     /locus_tag="IU325_02165"
     CDS             26866..27138
                     /gene="hypC"
                     /locus_tag="IU325_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HAP5807841.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            27138..28259
                     /gene="hypD"
                     /locus_tag="IU325_02170"
     CDS             27138..28259
                     /gene="hypD"
                     /locus_tag="IU325_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HAP5807842.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEEPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            28256..29266
                     /gene="hypE"
                     /locus_tag="IU325_02175"
     CDS             28256..29266
                     /gene="hypE"
                     /locus_tag="IU325_02175"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HAP5807843.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            29340..31418
                     /gene="flhA"
                     /locus_tag="IU325_02180"
     CDS             29340..31418
                     /gene="flhA"
                     /locus_tag="IU325_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HAP5807844.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYNRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            31463..31891
                     /locus_tag="IU325_02185"
     CDS             31463..31891
                     /locus_tag="IU325_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAP5807845.1"
                     /translation="MAVSARNQLTGTVSAVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(31930..32274)
                     /locus_tag="IU325_02190"
     CDS             complement(31930..32274)
                     /locus_tag="IU325_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HAP5807846.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYETLFTYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            complement(32351..32485)
                     /locus_tag="IU325_02195"
     CDS             complement(32351..32485)
                     /locus_tag="IU325_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5807847.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSVG
                     "
     gene            32561..35122
                     /gene="mutS"
                     /locus_tag="IU325_02200"
     CDS             32561..35122
                     /gene="mutS"
                     /locus_tag="IU325_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP5807848.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLENVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            35228..35884
                     /gene="pphB"
                     /locus_tag="IU325_02205"
     CDS             35228..35884
                     /gene="pphB"
                     /locus_tag="IU325_02205"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HAP5807849.1"
                     /translation="MPSTRYQKINAHHYRYIWAVGDIHGDYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFTSVKGNHEAMALDAFETGDGNMWLASGGDWF
                     FELNETEQQEAINLLLKFHRLPHIIEITNDIIKYVIAHADYPGNEYLFGKEIAERELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(35935..36732)
                     /locus_tag="IU325_02210"
     CDS             complement(35935..36732)
                     /locus_tag="IU325_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP5807850.1"
                     /translation="MSAGQRKEQKLIPVERRQIILEMVAEKGIVSIAELTDRMNVSHM
                     TIRRDLQKLEQQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQP
                     GSCIYLDAGTTTLAIAQHLIHMESLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENR
                     SCVGEAAATMLRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDA
                     TKYGQVATWLALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            36898..37806
                     /locus_tag="IU325_02215"
     CDS             36898..37806
                     /locus_tag="IU325_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HAP5807851.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            37803..39065
                     /locus_tag="IU325_02220"
     CDS             37803..39065
                     /locus_tag="IU325_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5807852.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSSAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDTPL
                     VTGGSGLAIGLARQWAQENGNQARKAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSIETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            39062..39700
                     /locus_tag="IU325_02225"
     CDS             39062..39700
                     /locus_tag="IU325_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP5807853.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            39705..40481
                     /locus_tag="IU325_02230"
     CDS             39705..40481
                     /locus_tag="IU325_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HAP5807854.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            40570..41934
                     /locus_tag="IU325_02235"
     CDS             40570..41934
                     /locus_tag="IU325_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HAP5807855.1"
                     /translation="MSTITLLCIALTGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(41973..42209)
                     /locus_tag="IU325_02240"
     CDS             complement(41973..42209)
                     /locus_tag="IU325_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5807856.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(42220..43647)
                     /locus_tag="IU325_02245"
     CDS             complement(42220..43647)
                     /locus_tag="IU325_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP5807857.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     VAPALWFDNIRGFIDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRSKPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(43647..44240)
                     /locus_tag="IU325_02250"
     CDS             complement(43647..44240)
                     /locus_tag="IU325_02250"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP5807858.1"
                     /translation="MRLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYNARDVAALADFCHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            44387..44794
                     /locus_tag="IU325_02255"
     CDS             44387..44794
                     /locus_tag="IU325_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP5807859.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDAADKRRRFVWLTAEGEKVLAAA
                     IPIGDSVDEEFLGRLSAEEQELFMQLVRKMMNT"
     gene            complement(44915..45907)
                     /gene="rpoS"
                     /locus_tag="IU325_02260"
     CDS             complement(44915..45907)
                     /gene="rpoS"
                     /locus_tag="IU325_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HAP5807860.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(45970..47109)
                     /gene="nlpD"
                     /locus_tag="IU325_02265"
     CDS             complement(45970..47109)
                     /gene="nlpD"
                     /locus_tag="IU325_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HAP5807861.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(47249..47875)
                     /gene="pcm"
                     /locus_tag="IU325_02270"
     CDS             complement(47249..47875)
                     /gene="pcm"
                     /locus_tag="IU325_02270"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HAP5807862.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(47869..48630)
                     /gene="surE"
                     /locus_tag="IU325_02275"
     CDS             complement(47869..48630)
                     /gene="surE"
                     /locus_tag="IU325_02275"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HAP5807863.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(48611..49660)
                     /gene="truD"
                     /locus_tag="IU325_02280"
     CDS             complement(48611..49660)
                     /gene="truD"
                     /locus_tag="IU325_02280"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HAP5807864.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(49657..50136)
                     /gene="ispF"
                     /locus_tag="IU325_02285"
     CDS             complement(49657..50136)
                     /gene="ispF"
                     /locus_tag="IU325_02285"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HAP5807865.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(50136..50846)
                     /gene="ispD"
                     /locus_tag="IU325_02290"
     CDS             complement(50136..50846)
                     /gene="ispD"
                     /locus_tag="IU325_02290"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAP5807866.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKHVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(50865..51176)
                     /gene="ftsB"
                     /locus_tag="IU325_02295"
     CDS             complement(50865..51176)
                     /gene="ftsB"
                     /locus_tag="IU325_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HAP5807867.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(51370..51693)
                     /locus_tag="IU325_02300"
     CDS             complement(51370..51693)
                     /locus_tag="IU325_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HAP5807868.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVIGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(51743..52348)
                     /gene="cysC"
                     /locus_tag="IU325_02305"
     CDS             complement(51743..52348)
                     /gene="cysC"
                     /locus_tag="IU325_02305"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HAP5807869.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(52348..53775)
                     /gene="cysN"
                     /locus_tag="IU325_02310"
     CDS             complement(52348..53775)
                     /gene="cysN"
                     /locus_tag="IU325_02310"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HAP5807870.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVGYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADESLPAV
                     QSASVDVVWMAEQPLSPGQRYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATTAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(53777..54685)
                     /gene="cysD"
                     /locus_tag="IU325_02315"
     CDS             complement(53777..54685)
                     /gene="cysD"
                     /locus_tag="IU325_02315"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HAP5807871.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            54937..55974
                     /locus_tag="IU325_02320"
     CDS             54937..55974
                     /locus_tag="IU325_02320"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP5807872.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEVFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGTSWHDVRLDNQQHIDKALPGRIERRCRDVIRIM
                     LPLVKELAKAS"
     repeat_region   56056..56816
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=56056..56084
                     /rpt_unit_seq="cggtttatccccgctgacgcggggaacac"
     gene            complement(56913..57206)
                     /locus_tag="IU325_02325"
     CDS             complement(56913..57206)
                     /locus_tag="IU325_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HAP5807873.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQISQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGVRLVSFLPVDNQ"
     gene            complement(57203..58126)
                     /gene="cas1e"
                     /locus_tag="IU325_02330"
     CDS             complement(57203..58126)
                     /gene="cas1e"
                     /locus_tag="IU325_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HAP5807874.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSSKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            complement(58123..58773)
                     /gene="cas6e"
                     /locus_tag="IU325_02335"
     CDS             complement(58123..58773)
                     /gene="cas6e"
                     /locus_tag="IU325_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HAP5807875.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(58755..59501)
                     /gene="cas5e"
                     /locus_tag="IU325_02340"
     CDS             complement(58755..59501)
                     /gene="cas5e"
                     /locus_tag="IU325_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HAP5807876.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNSHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSAPD
                     LLSAIISRRDYYTDAWWMVAVATTPDAPYRLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDVLRNAYQQYQDSFRELKVSLPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTINQGPWLSKEEPCTSQE"
     gene            complement(59512..60567)
                     /gene="cas7e"
                     /locus_tag="IU325_02345"
     CDS             complement(59512..60567)
                     /gene="cas7e"
                     /locus_tag="IU325_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HAP5807877.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTLRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTEQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(60582..61118)
                     /gene="casB"
                     /locus_tag="IU325_02350"
     CDS             complement(60582..61118)
                     /gene="casB"
                     /locus_tag="IU325_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HAP5807878.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGNRASLRRSTTVNDVCLSEGF
                     RSLLMQTHTLWKIEAQEWRFTALALVAAVSANVKAIDERQPFAAQLAAVMSEGRFTRL
                     SAVKTPDDLLRQLRRAVKLLNGSVNLISLAEDIFRWCQESDDLLNHHRRQQRPTEFIR
                     IRWALEYYQAGDADNEQN"
     gene            complement(61115..62677)
                     /gene="casA"
                     /locus_tag="IU325_02355"
     CDS             complement(61115..62677)
                     /gene="casA"
                     /locus_tag="IU325_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HAP5807879.1"
                     /translation="MNSFSLLTTPWLPVRYKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDQRRWDDIWEDGLEAEKLREALLSLDHAFQFGPDSPSFM
                     QDFEALTGDKVPVASLLPEIPGVQTTKFNKDHFIKRGVTEHLCPQCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWLNVMPQDEADLPLPKKFDD
                     LIFPWLGPTRTSELAGAVVTHDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICDEQNDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(62775..65432)
                     /locus_tag="IU325_02360"
     CDS             complement(62775..65432)
                     /locus_tag="IU325_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HAP5807880.1"
                     /translation="MTFFDFWGKTRRGEKDGGDDYHLLCWHSLDVAAMGYLMVKSNCF
                     GLTDYFRQLGFADTEQAAQFFAWLLCWHDTGKFARSFQQLYLHPQLKVPEGARKNYEK
                     ISHSTLGYWLWHHYLSEYEELLPSSSLSPRKLKRVMEMWMPMTTGHHGRPPDRIDELD
                     NFLPEDKAAARDFLLEIKVLFPLIEIPAFWDDDEGIELLKQLSWYISATVVLADWTGS
                     STRFFPRVAQAMDIKDYWQKALVQAQNALTVFPPKAQTAPFTGINTLFPFIEHPTPLQ
                     QKVLDLDISQPGPQLFILEDVTGAGKTEAALILAHRLMAAGKAQGLFFGLPTMATANA
                     MYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQAFSQGC
                     AAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYM
                     SCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGAEGQQEAPFLEKDDYPWL
                     THVTKSDVHSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQGKCIAWIRNSVDDA
                     IKVYRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKSCSLQRSSQVIVCTQ
                     VIEQSVDIDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILA
                     PVWDDAPGDEWFGSAMRNSAYVYPDHGRIWLTQRVLREQGAIQMPHAARLLIESVYGE
                     DVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDYLPEKLSTRLAEESV
                     SLWLSTCIAGVVKPYANGAHAWEMSVVRVRRSWWKKHRDEFSLLEGDAFRQWCIEQRQ
                     EPEMANVILVTDDESCGYSAMEGLTGKVG"
BASE COUNT        15451 a        17817 c        16971 g        15231 t
ORIGIN      
        1 ataacacttt taaatctttc aatctgatta gattaggttg ccgtttggta ataaaacaat
       61 aaattctgaa ggagagaaca atgatagaaa ccattactca tggtgcagag tggtttatcg
      121 ggctgttcca aaagggcgga gaggtgttta ccgggatggt gaccggcatt cttccgctgt
      181 tgattagcct gctggttatc atgaacgcac tgattaattt tatcggtcag catcgtattg
      241 aacgttttgc tcaacgttgc gccggtaacc ctgtttcccg ttacctgctg ctaccgtgca
      301 ttggcacgtt tgtcttttgc aatccgatga ccttaagcct gggtcgcttt atgccggaaa
      361 aatacaaacc cagctactac gcggccgcct cttatagctg ccactcaatg aatggcctct
      421 tcccccatat caacccaggc gaactgtttg tttatcttgg cattgccagc ggtctgacaa
      481 cactgaacct gccacttggc ccactggcgg tgagttatct gctggttggt ctggtcacca
      541 acttcttccg cggctgggtg accgatctga ccaccgccat ttttgagaaa aagatgggca
      601 ttcaacttga acaaaaagtt cacctggcag gagcaacatc atgacgcgta ttcggatcga
      661 aaaaggaacg ggtggctggg gcggcccgct tgagctggaa gccacgccgg gcaaaaaaat
      721 cgtctatatc accgccggta cccggcctgc gattgttgac aaactggcac agcttactgg
      781 ctggcaggct attgacggat ttaaagaagg tgaacccgcg gaggcggaaa ttggtgtcgc
      841 ggtaatcgac tgtggcggca cattacgctg cggcatttat ccgaaacggc gtattcccac
      901 cattaatatc cattcgacgg gcaagtccgg tccgctggcg cagtatattg tggaagatat
      961 ttatgtctct ggcgtgaaag aagaaaacat cactgtagtg ggtgatgcga caccacaacc
     1021 ctcttccgtg ggccgtgact atgacaccag caagaaaatc accgaacaaa gcgatggttt
     1081 actggcgaag gtgggaatgg gtatgggttc tgccgttgcc gtgttgtttc aatctggtcg
     1141 tgacaccatc gacactgtat taaaaaccat tctgccgttt atggcgttcg tctcggcgct
     1201 tatcggcatc attatggctt ccggtcttgg cgactggatt gcccacggtc ttgctccgct
     1261 ggcgagccat ccactggggc tggtcatgct ggcgctcatc tgctccttcc cgctgctttc
     1321 acctttcctc ggcccaggcg cagttatcgc acaggttatc ggcgtattga ttggcgtgca
     1381 gattggcctc ggcaatattc cgccgcatct ggctttacct gcactgtttg ccatcaacgc
     1441 gcaggcggcc tgcgacttca tcccggtcgg tttgtcgctg gcggaagccc gtcaggacac
     1501 ggttcgcgtc ggtgtccctt ctgttctggt gagccgcttt ttaaccggcg cgccaactgt
     1561 actgatcgcc tggtttgtct ccggttttat ctatcaatag aggctgaaac atgaccgtta
     1621 tttatcagac caccatcacc cgtatcggcg cgagtgccac tgacgccctc agcgaccaga
     1681 tgctcatcac ctttcgtgaa ggcgcgcctg cggatctcga agaatattgc ttcattcatt
     1741 gccacggcga gttgaaaggt gcactccatc ccggtttgca attttcgctc gggcagcatc
     1801 gctatccggt gaccgccgtt ggcagcgtgg cggaagacaa ccttcgcgaa ctgggtcatg
     1861 tcaccctgcg cttcgatggt ttaaacgaag cggaatttcc gggcactgtc catgtggcag
     1921 gccctgtccc cgacgatatc gcgccgggat cggttttgaa gtttgaatct gttaaggagt
     1981 aaaaaatgaa tcaggttgcc gttgtcatcg gtggtgggca aaccttaggc gcgttcctgt
     2041 gccacggtct ggctgccgag gggtatcgcg tcgcggttgt cgatattcag agcgacaaag
     2101 ccgcaaatgt ggcacaagaa attaacgccg aatatggtga aggaacagcg tatggttttg
     2161 gtgctgacgc caccagcgag caaagcgttc tggcgctctc tcgtggggta gatgaaatct
     2221 ttggtcgcgt ggatttgctg gtctacagcg ccggaattgc aaaagcagcc tttatcagcg
     2281 acttccagct cggcgatttt gaccgttcgc tacaagtgaa tctggtgggt tatttcctgt
     2341 gtgcccgtga attttcgcgt ttgatgatcc gcgacgggat tcaggggcgc attattcaga
     2401 tcaactcgaa atccggcaaa gtgggcagca aacacaactc tggttatagc gccgcgaaat
     2461 ttggtggcgt cgggctgact caatcactgg cgctggatct ggcggagtac ggcattacgg
     2521 tgcattcact gatgctcggt aatctgctga aatcgccgat gtttcagtcg ctattgccac
     2581 aatatgcgac caagctgggt atcaaaccgg atcaagtcga gcagtattac atcgacaaag
     2641 taccgctcaa acgcggctgc gattatcagg atgtgctgaa tatgcttctg ttctacgcca
     2701 gtcctaaggc gtcgtactgc accggacagt cgatcaatgt caccggcggt caggtgatgt
     2761 tctgatcaac agcggagatc cgttaaggat ctccgcgaga ctaataaaat gcctgatgcg
     2821 ctacgctcat caggcctaca ggacttccgc cactacatta aggaaaagtt atggtatccg
     2881 cactcatcac cgtcgccgtt atcgcctggt gtgcgcaact ggccttaggc ggttggcaaa
     2941 tttctcgttt taaccgtgct ttcgacacac tatgccagca agggcgggtt ggcgtgggcc
     3001 gttccagcgg gcgctttaaa ccgcgggtcg tggtcgccat cgcgctagac gatcagcaac
     3061 gcatcgtcga caccttgttt atgaaaggac tgaccgtctt cgcccgaccg caaaaaattc
     3121 ccgcaattac cggtatgcat gcgggtgatt tacagcccga tgtgatcttt ccccatgatc
     3181 cactatcaca gaatgctcta tcattggcgc ttaaactgaa acgtggataa tttcgttgtg
     3241 aatgttactt gcttgcgaag ttatcatttt gaaacctaaa tcaggtaatc acgcccatga
     3301 aacctcgtca gcgtcaggcc gccattctgg agtatctgca aaagcagggt aaatgctcgg
     3361 ttgaagaatt ggcgcaatac tttgacacca caggcacaac catccgcaaa gatctggtca
     3421 ttctggaaca tgccggaacc gtcattcgta cttatggcgg agtggtgttg aataaagagg
     3481 aatccgatcc gcctatcgat cataaaactc tcatcaacac ccacaagaaa gagctgattg
     3541 cagaagctgc cgttagtttt atccatgatg gcgattcgat cattcttgat gctggcagta
     3601 ccgttttgca gatggttccc ctgctctcgc gctttaataa catcacagtg atgaccaaca
     3661 gcctgcatat cgtcaatgcg ctatccgaac tggataacga acaaactatc ctgatgccag
     3721 gcggaacatt tcgtaaaaaa tcggcctcat ttcacgggca actggcagag aatgccttcg
     3781 agcatttcac cttcgataaa ttgtttatgg gcaccgacgg catcgatctc aatgcgggcg
     3841 taaccacgtt caacgaggtt tataccgtca gtaaggcaat gtgcaatgcc gcgcgcgaag
     3901 tgattttgat ggcagactca tcaaagtttg gccgtaaaag ccccaacgta gtttgcagtc
     3961 ttgaaagcgt cgataagcta attaccgacg caggtatcga tccggcgttt cgtcaggcgc
     4021 tggaaaagaa agggatcgat gtgatcataa ccggagagag caatgagtga agcactactg
     4081 aacgcgggac gtcagacgtt aatgctggag ttacaggaag caagccgttt accggaacgt
     4141 ctgggcgatg attttgttcg cgccgccaat atcatcctgc actgcgaagg taaagtggtg
     4201 gtttcgggaa ttggcaaatc gggccacatt ggtaagaaaa tcgccgcaac gcttgccagc
     4261 accggcactc ctgccttttt tgtccatccg gcagaggcat tgcacggcga cctggggatg
     4321 atcgaaagcc gcgatgtgat gctgtttatc tcttactccg gtggcgcgaa ggaactggat
     4381 ctgattattc cgcgtctgga agataaatct atcgcgctgc tggcgatgac cggcaaaccg
     4441 acgtcaccgc tgggcctggc ggcgaaagcg gtgctggata tttccgtaga acgcgaagcc
     4501 tgcccgatgc accttgcgcc gacttccagc actgtaaata ccctgatgat gggcgacgcg
     4561 ctggcgatgg cagtcatgca ggcgcgcggt tttaatgaag aagattttgc ccgttcacat
     4621 ccagccggtg cgctgggcgc tcgcttgctg aacaaagtgc atcatcttat gcgtcgtgac
     4681 gatgccatcc cgcaggtggc gttaaccgcc agcgtgatgg acgcgatgct ggaacttagc
     4741 cgcactggtc tggggctggt ggcggtatgt gacgctcaac aacaggtaca aggcgtcttt
     4801 accgacggtg atttacgtcg ctggctggtt ggcggcggcg cactcaccac gccagttaat
     4861 gaagcgatga cgacaggcgg caccacatta caggcgcaaa gccgtgccat cgacgccaaa
     4921 gagatcctga tgaagcgcaa aatcactgcc gcaccggtgg tggatgaaaa cggcaaactc
     4981 accggcgcaa ttaacctgca ggatttctat caggccggga ttatttaatc cttcagcccc
     5041 agacgtttcg ccagccgatg caggttggcg acgtcggttt ccagcatccg cgcacaggcg
     5101 gcccagttgt gatgattttg cgccagcgcc tgacgaatgg tttcacgctg gaacgcttct
     5161 gtcgcttcac gcaggttttg cttaacaacg ggcaccgccg ccacttctgg cggcggcaac
     5221 gtcacctcag gaaaagcaaa atgttgcgcc tcaagaatca cttcatcgcc gctgcgggtt
     5281 gctctcgcca gcactaccgc ccgatgaata gcatgttcca gttcacgaac gttccccgga
     5341 aaattgtagt gttgcagtaa atttcgcgct ccggcactta ataccacgcg ggagagcccc
     5401 agccgcaaac gacactgctc gcagaaatac cccgccagca gaatgacatc atcgccccgc
     5461 tcacgcagcg gcggcactga aagtggaaac acgctcaggc ggtggaacaa gtcagcgcga
     5521 aatcgccctg ccagtacctc ttcgcgtaag tcacggttag tcgccgccag cacgcgcaca
     5581 tcgacccgca aactgcggtc atcgccaacg cgctgaatat cgccatactg caacacccgc
     5641 agcagcttgg cctgcaatgc caacgacaac tcgccgatct catcgagaaa cagcgtgccg
     5701 ttatccgcca tttcaaactt cccgctgcgg ttactgatag cgccagtaaa cgctcctttc
     5761 acatgcccga acagttcact ttccgccaca ctttccggca gtgcggcaca gttgagatag
     5821 accagcggat tcaccgcccg tggcgaggct tcatgaatcg ctttcgccac cagctcctta
     5881 ccggttcccg tctcaccgct gatcaggacg ttgagatcgg acgccgccac aatctcaatc
     5941 tcttttttca attgcgtcat acctggcgac agaccgatca tctgcgtctg tttcactgct
     6001 tcaaacggcg cggcatcgcc tggcagcata ttctgacttt ccagttgttc aatcagcaac
     6061 gcattgctta acgctcccgc cgccagcgcg gcaatcagcc gtaactcttc gtcactgaaa
     6121 acatcgaact gatcgggctg catcccgtcg agcgtcaatg cgccgatcag gttttgtccg
     6181 gcaaacaatg gcagaccaac gcaggcgtga accttcagac tctcctgccc gggaatcaaa
     6241 ccgtcatagg gatcgggcaa ttcgctgtct gcgggaaagc gcaccacgtc cccggcgcgg
     6301 gcaatcgctt ccagccgtgg atgcccttcc agcgcaaagc gtctaccgag tacatccttc
     6361 gccagcccgt cgatggcaag cggaataaac tgccgcgagt cataacgtag caacgcagag
     6421 gcatcacact ccagcacctg acgcagcgtg gtgatcaggc gctgaaaacg atcctgatga
     6481 ccaatcccac gctgcaattc gatggcgata ttcgccagca catcaacgga aaaactcatc
     6541 tttgcctcac tgtcaatttg actatagata ttgtcatatc gaccatttga ttgatagtca
     6601 ttttgactac tcattaatga gcataatttt atttatagag taaaaacaat cagataaaaa
     6661 actggcacgc aatctgcaat tagcaagaca tctttttaga acacgctgaa taaattgagg
     6721 ttgctatgtc tattgtggtg aaaaataaca ttcattgggt tggtcaacgt gactgggaag
     6781 tgcgtgattt tcacggcacg gaatataaaa cgctgcgcgg cagcagctac aacagctacc
     6841 tcatccgcga agaaaaaaac gtgctgatcg acaccgttga ccataaattc agccgcgaat
     6901 ttgtgcagaa cctgcgtaat gaaatcgatc tggcggatat cgattacatc gtgattaacc
     6961 atgctgaaga ggaccacgcc ggggcgctga ccgaactgat ggcgcaaatt cccgatacgc
     7021 cgatttactg tactgccaac gctatcgact cgataaatgg tcatcaccat catccggagt
     7081 ggaattttaa tgtggtgaaa actggcgaca cactggatat cggcaacggc aaacagctca
     7141 tttttgtcga aacgccaatg ctgcactggc cggacagcat gatgacttac ctgacaggcg
     7201 acgcggtgct attcagtaac gatgctttcg gtcagcacta ctgcgacgag catctgttca
     7261 acgatgaagt ggatcagacg gagcttttcg agcagtgcca gcgttactac gccaatatcc
     7321 tgacgccgtt cagccgcctg gtaacgccga aaattaccga gatcctgggc tttaacttac
     7381 cagtcgatat gatagcgacc tctcacggcg tggtatggcg cgataacccg acgcaaattg
     7441 tcgagctgta cctgaaatgg gcggcggatt atcaggaaga cagaatcacc attttctacg
     7501 acaccatgtc gaataatacc cgcatgatgg ccgatgctat cgcccagggt attgcggaaa
     7561 ccgacccacg cgtggcggtg aaaattttca acgtcgcccg aagcgataaa aacgaaatcc
     7621 tgaccaatgt cttccgctca aaaggcgtgc tggtcggcac ttctacgatg aataatgtga
     7681 tgatgccgaa aatcgccggg ctggtggagg agatgactgg tttacgcttc cgtaacaaac
     7741 gcgccagtgc tttcggctct cacggctgga gcggcggtgc ggtggatcgt ctttccacac
     7801 gcctgcagga tgcaggtttc gaaatgtcgc ttagcctgaa agccaaatgg cgaccggacc
     7861 aggacgctct ggagttatgc cgtgaacacg gtcgcgaaat cgcccgtcag tgggcgctcg
     7921 cgccgctgcc gcagagcacg gtgaatacgg tggttaaaga agaaacctct gccaacacga
     7981 cggctgacct cggcccacgg atgcagtgca gcgtctgcca gtggatttac gatccggcaa
     8041 aaggcgagcc aatgcaggac gttgcgccag gaacgccgtg gagtgaagtc ccggataact
     8101 tcctgtgccc ggaatgctct ctcggcaaag acgtctttga cgaactggca tcggaggcaa
     8161 aatgagcaac ggcattgtga tcattggttc gggcttcgcc gcccgccaac tggtgaaaaa
     8221 tattcgcaaa caggacgcca gtattccatt aactctgatt gccgccgaca gcatggatga
     8281 gtacaacaaa ccggatctca gccatgttat cagccagggg caacgtgccg atgaccttac
     8341 ccgccagacg gcgggtgaat ttgccgagca gtttaatctg cacctgtttc cgcacacctg
     8401 ggtgacggat atcgatgccg aagcccatgt ggtgaaaagt cagaataatc agtggcaata
     8461 cgacaagtta gtgctggcaa ccggagccag cgcctttgtc ccgccagtgc ctgggcgtga
     8521 gttaatgctg acattaaata gtcagcaaga gtatcgcgcc tgtgaaacgc aactgcggga
     8581 tgcccgacgc gtattgattg ttggcggtgg cttgattggt agcgaactgg cgatggattt
     8641 ttgtcgtgca ggcaaagcgg tcacgctaat cgacaacgct gccagtattc tggcatcgtt
     8701 aatgccaccg gaagtaagca gccgcttgca gcatcggttg acggagattg gcgttcatct
     8761 gctgttaaaa tctcagttgc aggggctgga aaaaacggat tctggcattc tggcaacgct
     8821 ggaccgccag cgcagcatcg aagtggatgc ggtaattgcc gccaccggac tgcgcccgga
     8881 aaccgccctg gcacgacgcg ccgggctgac gattaatcgc ggcgtttgcg tcgatagtta
     8941 tctgcaaacc agtaatgccg atatttatgc gctgggcgat tgcacggaaa ttaacggtca
     9001 ggtattgccg ttcctccagc cgattcaact tagcgcgatg gtgctggcaa aaaatcttct
     9061 cggcaataac acgccgctga aactccctgc gatgctggtg aaaatcaaaa cgccagaatt
     9121 accgctgcat ctggcaggcg aaacccagcg tcaggattta cgctggcaaa ttaataccga
     9181 acgccaggga atggtggcgc gcggtgttga tgatgccgac cagcttcgcg cctttgtggt
     9241 cagtgaggat cggatgaaag aggcgtttgg attgttgaaa acgttgccgg tgtaggcatg
     9301 gttttctccc tcgcccaaag ggcgagggga ctgtccgcgc tctttttacg cttagttacg
     9361 ccacatccgc cactatgcag catgcaggat gcgcgccgca gcaataaccc cctgccccag
     9421 agacaagccg ccatcacccg ccggtaaact ctgcggaaag agtaatgtga aatcagcgag
     9481 ataatgcgcc aggcgtgtac gcagcaatcg gttatgaata accccaccgc taaataccag
     9541 cgtagtgata ccgcgcatcg tggcctgctc acgcatcaac gcggcaaaac cctgcgccag
     9601 cgcatcatga aacgcccacg cgcgttgatt aaccggtgcc tgccagttca gccactgctg
     9661 ccagaaagtg gcgagatcca gttgattgtc caccagcggc atcgtcaccg gatgcgtcac
     9721 tccgtcgcat gaggctgcga gcgcctccag agcacaagcc gcttcacctt cataacttaa
     9781 cgtggctggc gcacagccca gtgccgccgc cacagcatcg aacaaacgcc cacacgatga
     9841 cgccagcggc gcgttaattc cacgctcaat ggcccgcgcc agcacgctcc agttttgctg
     9901 ttgcacactt gtcgtttcag ggtaattctg ccactccggc acaaagcgca ggcactgcgc
     9961 cagcaggtta cgccacggct gctttgcagc caaatcgccc cccggaagcg ccaccgcagg
    10021 caagccgccc aggtgctcac attcgcgata gttcacccgc aggcattcgc cgccccacaa
    10081 agcgccgttc tcccccatac cgataccgtc gagcgtcaac gcaatgacat caccgccatc
    10141 cagcggccac tgatgctctg ccagactcgc cgccgcatga gcatgatgat gcagcaccgt
    10201 ttgcgtcggc agattcattt cgcgtgccca ctggctggag acatagcccg gatgcgcgtc
    10261 atgcacaacg tattgcggag tgaaatcgta gatgttttgc atcaggcgta acgcttcgcg
    10321 ccactgcatc tggatgccat cgtcacttaa atcgcccaga tgctgactca acaccgcttg
    10381 ttcaccgcgc accaggcaga aggtgttttt cagatccgcg ccgagacaca gcacaggcgg
    10441 aacattttta aagcccggag gcaaagccag cgcatccggc acataccccc gcgaacggcg
    10501 cagcatttcg ccgctttcgc gcaccaccga atcatccatc cgctgcacga tgtcgcggtt
    10561 atgtagcaag aatccgtcgg caatgccctg caaatccgcc agcgcctgtt cgttgctgat
    10621 agccggtggt ttaccgctca ggttgccgga ggtcatcacc agcggacatt gcagttcctg
    10681 taacagcaaa tgctgaagcg gattcgcagg caacatcacc ccaacttcgt taaggccagg
    10741 ggcgatatca tcacaaagct caggaacgta ttttttatcc accagcacaa tcggcgcggc
    10801 gggtgtggtg agcaactggc gcgcagcgtc tggtaaacct tctgccactg gcaacatgac
    10861 cgccagcggt ttcgccgggc gatgtttgcg cgcccgaagt gtcgccaccg cgttactgtt
    10921 acgtgcatcg caggcaagat gaaatccgcc aatccctttg atggcgacaa tgttgcccat
    10981 ttttaactgt gcgatagctg cctgtaatgc cgcctcttgt tccgcatgtt caccatgact
    11041 tacccattca agatgtgggc cacactccgg gcaggccacc ggctgggcgt ggaagcgacg
    11101 atcgagcggg tcgcggtact ctttgtcaca agccggacac aacggaaacg ccgccatcac
    11161 ggtaaacggg cggtcgtaag gcatggcgcg aataatggtg aaacgcgggc cacagtgagt
    11221 acagttgata aacggataac gataacgccg ctcgcctggg gtattcattt cggcaagaca
    11281 ggcagggcaa gtagcggcat cgggaacaat ttgcgtattc atggtgccgc ctgtgctctg
    11341 gcgtatagtg aactcggtgg gcagttgtga ccagataaac ggctcacgct cgacgctatc
    11401 aatacgcgcc agcggcgggc agtgctgatg caactgcaca agaaacgttt ccgggtcttc
    11461 cagcagccgg acttctacgc catcgccgtc attacagaca tcgccgtgaa gatttaattg
    11521 ctgtgccagc tgccagacaa acggacgaaa accgacgccc tgcactttgc cacgaatacg
    11581 cagttggaca ccgcaagatg tgttttttgc cattgagtta ttcccgccat catgaattgc
    11641 gtaacccacc ctgccggaca cgacagcgtc gtatccggca atcacaggtc ggcgataccg
    11701 cccgctccgt attctacgaa tatttccggg aattcctttg atgccagaac agttctgtaa
    11761 gatttttaga acatcagcgc cgtacggcgg cgtttttctg cgctcagttg ttcaagttta
    11821 ttacgatcga cacaaatcag cgcatgggtc gggcaagccg ccatacacgc cgggccgtct
    11881 tcacgatggt tacagaggtc gcatttattg gcttcggctt tgtcagcccg tacattcagc
    11941 cctgcgccgc tgttgcgaat caccggacgt accaccactt ccatcgcgcc atacgggcaa
    12001 gccacaacgc aggttttgca accaatgcaa cgttcctgca tcacatgaac aaacccttta
    12061 tcacggctga tagcaccatt tgggcagacg ttagcgcacg gtgcatcttc acactgacgg
    12121 cagactgttg ccgtagaaat gttcacacct ttaatgacat ggattcgcgg taaaaaagtt
    12181 tccggggtca gcgatgcaca gtcctgattc tcctgatgag aaaccacgca cgccacttca
    12241 caggtacggc aaccaataca tttactcgcg tcagcaatga tgaaacggtt catcaaattc
    12301 tccagcaatg acagttaatg cgccgataca ttcacaaatc atgccagttt ttaatttact
    12361 gttatttaaa gaaattaaat tctgtaatgc aggaaaaacg atgtcatcga cactagtgac
    12421 gatgacatgt gatgacaatg tttattgcga aagagcaatg agggagccgc ggcggatcag
    12481 ttttccgctg aaggttttcg gcggtgagaa atccccacca tcgagcataa aaatcagccg
    12541 tccaataatt tcctgaatca tctcagtcac cggaattttt acgctggaga gcgccggaac
    12601 gatatagggg gcaatggcga tatcatcgaa tccgataact gacacctgct ctggcaccgc
    12661 tacgccgcgc tcgtgtaacg ctttcatcgc acctatcgcc atatcgtcgt tactggcaac
    12721 taacgcgcta aatttagccc cacgttcgag caacgtttct accccttcgg ccccgctggc
    12781 aggcgtccat ttaccgttag cgataagttt ttcattgagc gcaatactat gctgcgccag
    12841 cgcgtcttta tacccggcaa gacgttcaat gctggtggga gaatccatcg agccggtaag
    12901 gaaagcaatc tcctgatgtc cggcgtttat caactctgcc acggcgttaa agctggtctg
    12961 tttatgatcg caccagacgc tatggctgct gtttttgcgc aggcggcgat taagcaccat
    13021 tatcggctga ctgtgtgcgt caatgatgtc atcaatctca tccacgctta aaaaacgcgg
    13081 gtaaatcata atggcgtcgc agcgcaaatc cagcagatac tgaatcgcct ggcgctcttc
    13141 ttcggcgctg tgtttaccat ctgccaatag caactgtcgc cctttctctt ccgccattcg
    13201 cgcggcatga aagagtaatt cactaaaata aatgccgtgg taaagcgtgt tggtcacgac
    13261 cagccccagc gtctgagtac tcttcgccga cagattgcgc gccagcaagt ttgggcggta
    13321 accgctctct tctaccgcct gaaacacgcg atctttagtc tcctggctga cgtagccatt
    13381 acctgaaagc acgcgggaaa cggtcgcttt tgaaaccccg gcgcgcttcg ccacttccag
    13441 catcgtcgtc atcattttca tccctttaca cgcaatcaac gcagtgtact gcaccgtttg
    13501 ccgattgtcc ttgcgcaatc ggcgggaaaa atattcaggt gaccggtttc acaaatataa
    13561 aaaatgaaca attaactctc ttgcttattt agtgacaact attcatgatt ttgtgaaacc
    13621 ggtttcctaa ttccgtttca gcatcggcat ttttccgtca cgtcgactga taacaactac
    13681 atctacccta ctgataacag gataaaatcc gatggccaaa aattatgcgg cgctggcacg
    13741 ctcggtgata gcggcactgg gcggcgttga taacatctcg gcagtcacgc actgtatgac
    13801 gcgtttgcgc tttgttatca aagatgacca gcttatcgac agtccgacgt tgaaaaccat
    13861 ctccggcgtg ctcggcgtgg tacgtagtga caaccagtgc caggtgatta tcggcaatac
    13921 cgtttcacaa gcctttcagg aagtcgtcag cctgctgccg ggagatatgc agcccgcaca
    13981 gcccgtgggt aaacccaaac ttacgctacg tcgcattggt gcggggatcc tcgatgcgct
    14041 gatcggcacc atgtcaccgc tgatcccggc gattatcggc ggatcgatgg tcaaactgct
    14101 ggcgatgatc ctcgagatga gcggcgtgct gacaaaagga tcgccgacct taaccattct
    14161 gaatgtgatt ggtgacggtg ctttcttctt cctgccgctg atggtcgcgg cttctgccgc
    14221 catcaaattt aaaaccaata tgtcgctggc gattgccatc gcgggtgtgc tggtacatcc
    14281 gagttttatt gaattaatgg cgaaagcggc ccagggtgaa catgtcgaat ttgccctgat
    14341 tccggtcacc gcggtgaaat acacctacac ggtgatcccg gcgctggtga tgacctggtg
    14401 cctgtcatat atcgaacgct gggtggacag cattacaccg gcggtgacta aaaacttcct
    14461 caagccaatg ctgattgtgt tgattgccgc accgctggca attctgctga ttggcccgat
    14521 tggtatctgg atcggtagcg ccatttcggc gctggtttac accattcatg gttatctggg
    14581 ctggctttca gtcgccatta tgggcgcgct gtggcctctg ctggtaatga ccgggatgca
    14641 ccgcgtcttt acgccaacca tcattcagac cattgccgaa accggcaaag aagggatggt
    14701 catgccgtca gagatcggcg ctaacctgtc gctgggcggt tcatcactgg cggtggcgtg
    14761 gaaaacgaaa aacccggaac tgcgccagac ggcgctggct gcggcggcat cagccattat
    14821 ggcggggatt tccgaaccgg cgttatatgg cgtggcgatc cgcctgaaac gtccgcttat
    14881 cgccagtctt atcagcggtt ttatttgcgg cgcggttgcc ggtatggcgg ggcttgccag
    14941 ccactcaatg gcagcgccgg ggctatttac cagcgtgcag ttcttcgatc cggcgaatcc
    15001 aatgagcatc gtctgggtgt tcgcggtcat ggcgctggcg gtggtgctgt cgtttatcct
    15061 cacactgttg ctcggctttg aggatattcc tgttgaggaa gcgactgccg aggcgcgaaa
    15121 gcatcagagc gcacaaccga ccgtcgccaa agaagtaagt cttaattgag gatgaaaatg
    15181 tcagtatttc cagaaggttt tttatggggc ggcgcgcttg ccgccaacca gtctgaaggt
    15241 gcgtaccgtg aaggtggcaa agggctgacc accgtcgata tgatcccaca cggcgagcat
    15301 cgaatggcgg tgaaactggg gctggaaaaa cgttttcagt tgcgcgatga cgagttttat
    15361 cccagccatg aggcgacgga tttttatcat cgttataaag aagatatcgc cctgatggca
    15421 gagatgggat tcaaagtgtt ccgtacctca attgcctgga gccgcctctt cccgcagggc
    15481 gatgaactga cgcccaacca gcagggcatt gctttttacc gttctgtctt tgaagagtgt
    15541 aaaaagtacg gtatcgaacc gctggtcacg ttgtgccact tcgatgtgcc gatgcatctg
    15601 gtcaccgaat atggctcctg gcgtaaccgc aagctggtgg agtttttcag ccgctacgcc
    15661 agaatctgct ttgaagcatt tgatggtctg gtgaaatact ggctaacctt caatgaaatc
    15721 aacattatgt tgcatagccc gttctccggc gcgggtctgg tgtttgaaga aggcgaaaat
    15781 caggatcagg tgaaatatca ggctgcgcat caccaactga ttgccagtgc attagccacc
    15841 aaaatcgccc atgaggttaa cccgcaaaat caggtgggct gtatgctggc gggcggtaac
    15901 ttctaccctt acagttgcaa gccggaagat gtctgggcgg cgctggagaa agatcgggaa
    15961 aacctgtttt ttatcgatgt gcaggcgcgg ggcgcgtatc cggcttactc tgcccgcgta
    16021 ttccgcgaaa aaggggtaac catcgacaaa gcaccgggcg atgatgagat cctgaaaaac
    16081 accgtcgatt ttgtctcttt cagctattac gcctcacgct gcgcctcggc ggagatgaac
    16141 gccaacaata gcagtgcggc gaacgtagtg aaatcgctgc gtaacccgta tctgcaagtg
    16201 agcgactggg gctggggtat tgatccactc ggtctgcgta tcaccatgaa tatgatgtac
    16261 gaccgttatc agaagccact gtttctggtg gaaaacggcc tcggcgcaaa agatgaattt
    16321 gctgccaatg gtgagattaa cgatgactat cgcatcagct atttacgcga acatatccgc
    16381 gcaatgggcg aagcgattgc agatggcatt ccgctgatgg gctacaccac atggggctgt
    16441 attgatttag tttccgcctc tacgggtgaa atgagcaaac gctacggttt tgtctacgta
    16501 gaccgtgacg acgtaggcaa cggcacgctg acgcgcacac gtaagaaatc gttctggtgg
    16561 tataaaaaag taattgccag taatggtgaa gatttagagt aagtaactac cgtcgcacga
    16621 accaggaaag cccgataagc gttgcgcatc gggcaatctt gggtttggca tcagtcaaaa
    16681 tcaaagaaac cattccggct taacgccaaa tcgggctgaa agtgccttaa tatgggatat
    16741 cgtcagggat cgttgcccag acaaaatctg actgaccaga gatttagagc cgatttcgtt
    16801 cttcaaatcc gcataggtca aattatgttg atcaatcagc gtacgaagta aggccacgcc
    16861 aaccggcatt gctgcaacgg ctttgtcgaa ctctgcaaac ttttcgttgt tgttttcata
    16921 ttctgcaatg cggctcgcca gaaagtcgat aagtggattg gtgtcatctt cttcaatcaa
    16981 atattcgacc agttccagtg cttcacgata atcatcttct gagtcactgc ctcccagaaa
    17041 ggggactgca ttgaccagtt ctcttgttgc tttaacagcg cgtgcagcat tagcagtcat
    17101 tctttgttct ctctgtagta acgtgtcagt ttgtcatact ctgcgtgatt cgttatgtgc
    17161 ttcacaaaga accgcttatt aacaaagttt atgtaggcaa taaccctaag attgttcccg
    17221 ccgacatcca gaacgtacca tttgttgcga tatttaaaat tgtcgaggtt aggaaacgcc
    17281 gttcgcattt cttctggtgt agaaaaatcc gtttccttaa taacccgata caatgagtgt
    17341 agagccagag cgtcgttagg atatttgcgc gcacactctt caaatggcgc tttcgaaatg
    17401 atgtgcattt taacttcctt attagcgcct gaatgactaa acctggacta ccattccttc
    17461 ggatttattt ttctatagta gttgatcaca cgggtgttca caatatgtaa acacccgtct
    17521 acacgtcaca attgcacgtc caaattccaa aacgtgaaca tgtttgtcta cgaactactc
    17581 ttcttccacc gctaactgcg cgaatccgcc attcccttcc cagccttcca gtcgttgata
    17641 aacggtttct acggcgtctt taatcggctg ggtcatcggg tagtaaaagc cgacgatatc
    17701 cggctggatg ccgaggaaaa tcacttcgcc aatgtcttct ttcaactggt cgataaggta
    17761 gttcaacggc atgttatggg tagtcatcat aaacatctcg gcgatatcat ccggatcgat
    17821 gatgcggatc tcgccggggt ttagccccat atccgtggcg tcgacaatca acagtcgtgt
    17881 cgggcgcagt tcgcgaatag cgacgatgtc gttttccggt gcgctaccgc cgtcaatcac
    17941 cacccagtta cctttcggcg cggcggcaca cttttccgcc agcagcggac ctgcgccatc
    18001 atcgcccatc atgctattgc caacacagag taaaacgtca gtcacgcaat ctcctcacca
    18061 tcaaatagat ggcgttttcc tgatgaatat catgcagcat acttaacagc attgtactcc
    18121 attctttctg ctgcgaagtt tgcgcagccc gggctttatc gaatgcgttg gccagcatcg
    18181 gcacatggtt aatgtcgatg acgatctcac catatttcgg cacgccttcc attttgcggc
    18241 gggcttcgct gcccgcctcc agagtggcaa tccatgccag atattcatcc cacggacagg
    18301 tgagcgccgc ttccaggcaa tcgataaccc caaggtggtg accaatcgcc aggctgtaat
    18361 agaccacctg ctgcgcctcg gcgggcgtgg catcgttctc atcaataaat ttacggctca
    18421 gttgactgaa caccaccttt tcactcatcg gatacgcgcc tcttcaacaa catgattcag
    18481 atggctgaca atctcgttca gacgcggatc gttttccgct tccagccagc gtgcaacctg
    18541 ctcttcgccc tgccctaact gtgtcaggaa gtcatcggca atctggcgac cgtaacggta
    18601 acccgccagg cggcgagctt cgcgatccac tttcacgcgc agcggctgca ccatatcgcc
    18661 atgcaggatc tccgccggtt gttcatcctg ctcacccggc ccacgggcgt gaattttctg
    18721 ctccagcagg ccgagcgcca ttgcaaagcc gtacagcgtg gcggcaggcg ttggcgggca
    18781 gccagggata taaacatcca ccgggacgat tttatccgta ccgccccaca cgcagtagag
    18841 atcgtggaag atcccgccgc tgttaccgca ggcaccgtag gagatacaaa ttttcgggtc
    18901 cggcgcggac tgccacgcac gcagcgcagg ggatcgcatt gcacgggtga ccgcgccggt
    18961 aaacagtaaa atatccgcat gacgcggtga aggaacgact ttaatgccga agcgttctgc
    19021 atcaaacagc ggcgaaagcg tggcgaaaat ttcgatttcg cagccgttgc agccgccgca
    19081 gtccacgcgg taaacgtagg cagaacgttt gatttttttc agtaacgacg ccttcatgct
    19141 ggcgatggat tcatccaccg tcatcgggac cggaatgccg ttggcgtcac gggggcctaa
    19201 taaattgctc atcagatggc ctctttcata tggcgagtca gttcaatacg gtcggacggc
    19261 accaggcatt tctggcgctt acattccggg caagtctcaa agctttcgcg gtggttttcc
    19321 gcgcggctgt cgccgttgtg cttgagcagc gcaatggcgt agtcgatctc tttctggacg
    19381 gcgaaaggac gattgcagac gcgacagttg cacagcgcga agcgggactg ttgcaggaag
    19441 tcttctttct tccacaccgc cagttcgtac tcttgcgaca gcttaatcgc cgccgtcggg
    19501 caaacttctt cgcagcgtcc acagaagatg cagcgcccga gattaaactc ccaggcaagc
    19561 tctcctgtgg cgaggtcagt ttcaaccgtt aaggcgtttg acgggcaggc attgacgcag
    19621 gccgcgcagc cgatgcactg ctgcgggttc tgctctggct taccacggaa gtttttatca
    19681 accgcaatcg gctccagcgg ataagacgag gtcgccgtgc cggttttgat aacttttttg
    19741 ataaaggtaa acatggcgat tccttatttc agcggcgagt ttttacgctc aatgctgtaa
    19801 cgctcgagtt ctttgtacgg caccactttg ctcttcttct tacgcacatc gaccacggtc
    19861 atgcggtcgg tacaggagta gcaagggtcc aggctaccga taatcagcgg cgcatcggaa
    19921 acggtgttgc cgcgcagcat gtagcgcagg gtcggccagt tcgcgtaggt cgcggcacgg
    19981 cagcgccagc ggtacagctt ctggttgtcg ccggtcatgc tccagtggat atcgtcgccg
    20041 cgcggcgctt cggcaaagcc cagcgcaaaa cggtgcggaa tataggtaaa gccttccacc
    20101 atcagcggac cgcccggcag gttatccaga ccgtagtcga tcatgttcag cgcggtatag
    20161 acctcgttga tacgcacttt cagacgggaa ataacgtcgc agccctgctc gctgtggact
    20221 tccattggca gcaggccata accaacaaac gggtgatcgg cacgggtatc gcgggcgtga
    20281 ccgctggcgc ggaccatcgg gccaacgtta ctgaaatcgc gggcgatttc cgggtccaga
    20341 cgaccaatgc cgacagtacg ctgttccatg ttcggtgtgc tcagcagcac atccaccagc
    20401 tcctgcactt cacgacgcat ctgttgcgcc aactggcggg tctggatcat gtcatctttc
    20461 agcagatcgc gacgaattcc gccgatcagg ttcaggccgt aggttttacg cgcaccggta
    20521 aggatctctg ccattttcat ggaggtttca cgcacgcgga agaactgcat aaagccggag
    20581 tcgaagccgg taaagtgaca ggcgaggccg aggttgagca gatgcgagtg caggcgttct
    20641 acctccagca gaatggcgcg gatcatctgc gcacgttctg gcacctgaat acccatcgcg
    20701 ttttccaccg acgtggtgta ggcggtgctg tgagcaaagc cgcagatccc gcacacacgg
    20761 tcagagagga aggtcacttc gttgtaaccc atgcgggttt ccgccagttt ttccatgccg
    20821 cgatggacat agaacaggcg gtagtcggcg tcgataatgt tttcgccatc gacgaacaga
    20881 cggaagtgac ccggttcgtc ggaagtgacg tgcagcggac caatcggcac gacgttgttt
    20941 ttcttgtcgc ccagttcgtt gatgaactcg taggtttcag catcggtagt cggtgccgga
    21001 cgttgacgat aatccatgct gtctttacgc agcggataaa gttcatccgg ccagtcatcc
    21061 ggcagcacca gacgacgttc atccggcagc cctaccggaa tcaaaccgta catatcgcgc
    21121 acttcacgct cgccccatac cgccgccgga acgcgcggcg ttacggacgg atattccggt
    21181 ttgttggcgt caacttcaac gcgcacggtt atccagcatt tggtgccctt ctccatcgac
    21241 agcacgtagt aaacggcgta atgaccatta agtttgcgtt cgtcgttacc aaacagcacc
    21301 gacagccagc ccccctgctt gtagtagaga aactccacca cttccggcag gtagttcacc
    21361 ttcacggtca cggtcagctg atctttggtc tgccaggcgt ggtccagcac gacgcccgga
    21421 aatgcctcat tcagcgcagc gagataatgt tgacctaatt tttcttcaga catgctcaaa
    21481 ctctctttaa tcacgccgcc agcaaggaga cgaacgctaa aaatgcaaag ccaaacccag
    21541 cccaggtaat gcgcggagta atatcaagac gcagacgggc catgctgttt tcgaacagcg
    21601 cgataaccag gacgccaacc accagtttga cgatggcaat caccagcgcc agcagcagcc
    21661 caccgacggt gaaggtttcc atttgtcccc acggaataaa caccccgacg aacatctgca
    21721 acaccaccag ctgtttcagg ctgatacccc atttcatgac gccaaagccg ctgccgctat
    21781 actcagagag cggaccttcc tgcaactcct gctcggcttc cgccaggtcg aacggcagtt
    21841 tgcccatttc gataaaggtg gcgaatgcac aggcacaaag cgccagcacc agcggaatgc
    21901 tctggctcag cggccagtga taaacggtgt cggtgatgtt gctgatgttg gtggaaccgg
    21961 caacctgtgc ggcgacccac agaccaagca gcagcatcgg ttcaaccagc acgccaagca
    22021 tcgcttcacg gctcgcgccg atagcggtaa acgggctacc ggtatccaga ccagaaatgg
    22081 caaagaagaa acgcgcgatg gcaaagagat acagtaaagt gatcaaatcg cccagctgcg
    22141 gcagcggaga accaacggtc accaccggca gcgcagtagc gatagtcagc atgacgccca
    22201 ccatcacata cggcgtcagg cggaacaccc agccggaggc atccgggcca acgctctggc
    22261 ggcccagcag tttgataatg tcgcgatact cctgcaacac gcccggcccg cgacggttat
    22321 gcaagcgggc gcgcgccacg cgggttatac cggagagcag cggcgcaacg gcaaataaca
    22381 ccagcgcctg aattaacgga tataaaacac tcattctcag gctcctcgtg aaacaataat
    22441 caccaccagt accgccagtt caaccagcgc catccggcgg aacagcaacg cactcccctc
    22501 gcactgccag cccggtacca gcgacaccgg attcagccat ttgcgcagtt tcagcaccgg
    22561 cgcaaacgcc tgtttcaccg gcatggcaaa accgtgagca gtaatcacca ttgatttttc
    22621 atggtcgtag ccgcacaccc aggccgcgcc gcgggaacgc gacggcaaac gatcgccttt
    22681 gcaaatcgcc ataatgatga atggcagcag cgggcaggca atcagcagca acgtgatcat
    22741 gggttgagaa acggtggtgt tagcaggctc cagcggcaga ggtacagcag cagagagcat
    22801 cggcagcagc cacggcgcag caacaccgcc aattacgcag caaatcgcca gtgccaccac
    22861 gcttacgctc atcaggagcg gcgcactggt ggcgttttcg gcttctttgg tacgcggcgc
    22921 gccgaggaac gtgacgccat agactttcgc catacacatc accgccagcg caccggtaat
    22981 tgccagaccc actgcgagca gcggccccag cagacgggca acaaacgcgc cactattgct
    23041 cagtttgaag aaggattgat agataaccca ttccccggca aacccgttca gcggcggcaa
    23101 cgcagccatt gccatcagcc cgactaacat ggcgatggag ataaccggca ttttcttgcc
    23161 aataccaccg agtttttcga tatcgcgatg accggtacgg aaccagacgc tccccgcccc
    23221 gaggaacagt acgcttttga acaggctatg gttaagcaga tggtacagac caccgaccag
    23281 gccaagagca atcagcgccg gttgttcgag cgcgataccc gttacgccag cgcccagccc
    23341 cagcaggata atgccgatat tctccagggt gtggtaagcc agcaggcgct ggatgttgtg
    23401 ttccatcaac gcatacagac caccgacgaa cgcggtgatc atgccgagca ccagcagcgc
    23461 gatcccccac cacagcggtg cattaccgcc cagcagcgac agggttaaaa tgcccagcag
    23521 gccaattttc atgactaccg tagaaaacaa cgcggcagct ggcgcagagg cgttagcatg
    23581 tgcctgcggc acccagccgt gcagcggaat aatcccggcc agcaggccaa agccaatcac
    23641 accgagcagc cagatatcgg aaccgagcgg cagctgttgc atacgcatat ccagcaggcg
    23701 cagatccagc gtgccgtaac gctgccacag cagccagcag gcaatcgcca gcagcagagt
    23761 gccaagacgc cccagcgcaa accacagttt gccctctttg ctgttgctgg tgaggaacac
    23821 cgcacacagg gccatgattt cggccattac cacgaacatg ccgaggttgc tggcgatgac
    23881 ggcacagacg gcggcagcca tcaacatatt gatctgcaag ccgttgcatt tcacctgcgc
    23941 gtggcgatgc cagtcaatgt tgtagaggct gacaaacaga ccgcacaggc cgagcgtaat
    24001 cagccaaatc gcgttaagcg gagagatttg cacatcgtag cttaccagcg acagcgcacc
    24061 gctcacgcca accgcaccag tcagcacagt gaagcccgcg gctgccgtat acaggctacc
    24121 caccgcgccg ccaattccgg ctatccagcc gcttagcgcc ttttgaaaag aaaagagaaa
    24181 tgccagaaca gcggcggcgg caaaccacgc cacgccacta ttgatcaggg aaattgcgct
    24241 catttagcct ctccactttg agcctgctga aacaaggtga gatcgccaaa gtccgtgtta
    24301 aaagtcagct cacgcttacg tttgctgacg cgggcgatat cggtgttatc caccagatgc
    24361 agggctttag tcgggcacat ccgcacgcag gccggacctt gttcatcaaa gctacaaagg
    24421 tcacatttga cggcgatcgc gcgaatacct ggcacccagt caagcaatgt gctgacacgc
    24481 gccggagcag gcggtgccgg tggcgctttc ggggtattgg cgtttgccgg aatatccagc
    24541 ggacggctgc cggaaaattc aattgcgcca aacgggcagg cgatgccgca cagtttgcag
    24601 cttacgcaca gactttcatt caactgcacg gccccatcaa cgcgggtgat ggcgttaacc
    24661 gggcaaacca ccgcacaggg cgcatcttca cagtggtgac agagctgcgg cgcagattct
    24721 ttttcattga gcatcactcg caggcgcggc attgattgca ggccgtgctg gcgatgcgtc
    24781 tctgaacagg cggcctcaca ggtgtggcag ccgatacaga gcgtggagtc agcaattaca
    24841 aaacgattca ccaggcattc ctcaggtgat tgtcattttt gacgaaaaca tgccgatgaa
    24901 atgtcatttt cgacactcat cgacacgccc atccccaaac aggcgtaacg cctgcaaaac
    24961 gggcaaagcc tcagctcatg ctgccgggct ttgtcccttt accagttggc ttaaattcac
    25021 cggcacattg ttttcaatgg cggtgtataa gctgtcgtaa acgccagcga ttttttcgag
    25081 atagtgttcc agaacctgtt cgcgatcgcg gatgttgacc ttgccatcaa tcatgccgtc
    25141 aatgctcagt tgcgcctgcg caatccgctg tttgtcttca ccatcttttg tttcgacgta
    25201 atactcgatg gtgtggctgt taaagccatc cgccagggtg acgtaaatgc gaaaatgttc
    25261 aaaaaggacg aaacagagtt ctttgtccgg cgcgcagctc atggcgtgat gcaggcgcgc
    25321 tttcgataac gtgatcccct gaaccagcga attgcagtag atgtgccact ggtcctgtag
    25381 gcgacgatgc cgctgtgcga tgtaatcggc tttctcgctt atttcccaaa tagtcattgt
    25441 caggttaccc gtttaacaga gatgccagct ttaagcattt tctgtgccaa cttttaattc
    25501 attgttatga aacagatttt taagataaag atgaatttcg tcgccgtgtc gacgtgtcat
    25561 ttcgacatca tcgacattat tcaccgcagg gataatcaac actggcacaa ttattgcttg
    25621 tagctggcaa tagttaatgg gaggcgatat gcacgaaata accctctgcc aacgggcact
    25681 ggaattgatc gaacagcagg ccgcaaaaca cggcgcaaaa cgcgtaactg gggtctggct
    25741 caaaattggc gcattttctt gtgtcgaaac cagctctctt gccttttgtt ttgatctggt
    25801 ttgccgcggc agcgtggcgg aaggttgtaa actgcacctc gaagaacaag aggccgaatg
    25861 ctggtgtgaa acatgccaac agtatgtgac gctactgacc cagcgcgtcc gccgctgtcc
    25921 acagtgtcat ggtgacatgc tacagattgt ggcagacgac ggtttacaga ttcggcggat
    25981 agaaatagac caggagtgag cgatgtgtac aacatgcggt tgcggtgaag gcaacctgta
    26041 tatcgagggt gatgaacata accctcattc cgcgtttcgt agcgcgccat ttgccccggc
    26101 ggcacgcccg aagatgaaaa tcaccggcat taaagcgcct gaatttaccc ccagccagac
    26161 tgaagaaggc gatctgcatt acggtcatgg cgaagcgggt actcacgcgc cgggcatgag
    26221 ccagcgtcgg atgctggaag tcgaaattga cgtgctggac aaaaataacc gtctggctga
    26281 acgcaaccgc gcgcgctttg ctgcccgcaa gcaactggtg ctcaacctgg tttccagccc
    26341 tggttccggt aaaaccaccc tgctgacgga aaccttaatg cgcctgaaag acagcgttcc
    26401 gtgcgcggtt attgaaggcg accagcaaac cgtgaacgat gccgcgcgca ttcgcgctac
    26461 cggcacacca gcgattcagg tgaataccgg taaaggctgc catcttgacg cacagatgat
    26521 tgccgacgcc gcaccgcgcc tgccactgga cgataacggt attctgttta tcgaaaacgt
    26581 cggcaacctc gtgtgcccgg ccagcttcga tctcggtgaa aaacacaaag tggcggtgct
    26641 ttccgttacc gaaggtgaag acaaaccgct gaaatatccg catatgtttg ccgcagcttc
    26701 gctgatgctg ctcaacaaag ttgacttgct gccgtatctc aactttgacg ttgagaagtg
    26761 catcgcctgc gcccgcgaag tcaatccaga aattgaaatc atccttattt ccgccaccag
    26821 cggcgaaggg atggaccagt ggctgaactg gctggagaca cagcgatgtg cataggcgtt
    26881 cccggccaga tccgcaccat tgacggcaac caggcgaaag tcgacgtctg cggcattcag
    26941 cgcgatgtcg atttaacgtt agtcggcagc tgcgatgaaa acggtcagcc acgcgtaggc
    27001 cagtgggtac tggtccacgt tggctttgcc atgagcgtaa ttaatgaagc cgaagcgcgc
    27061 gacactctcg acgccttaca aaacatgttt gacgttgagc cggatgtcgg tgcgctgttg
    27121 tatggcgagg aaaaataatg cgttttgttg atgaatatcg cgcgccggaa caggtgatgc
    27181 agttaattga gcatctgcgc gaacgtgctt cacatctctc ttacacagcc gaacgccctc
    27241 tgcggattat ggaagtgtgt ggcggtcata cccacgctat ctttaaattc ggcctcgacc
    27301 agttactgcc ggaaaacgtt gagtttatcc acggtccggg ttgtccggtg tgtgtactgc
    27361 cgatgggtag aatcgatacc tgcgtggaga ttgccagcca tccggaagtc atcttctgta
    27421 cctttggcga cgccatgcgc gtgccgggga aacagggatc gctgttgcag gcaaaagcac
    27481 gcggtgccga tgtgcgcatc gtttactcgc cgatggatgc gttgaaactg gcgcaggaga
    27541 atccaacccg caaagtggtg ttcttcggct taggttttga aaccaccatg ccgacgacgg
    27601 ccatcactct gcaacaggcg aaagcgcgtg atgtgcagaa tttttacttc ttctgccagc
    27661 atattacgct tatcccgacg ctgcgcagtt tgctggaaga gccggataac ggtatcgacg
    27721 cgtttcttgc gcccggtcac gttagtatgg ttatcggcac tgatgcctat aattttatcg
    27781 ccagcgattt tcatcgtccg ctggtggtcg ctggtttcga accccttgat ctactgcaag
    27841 gcgtggtgat gctggtggag cagaaaatag cggcccacag caaggtagag aatcagtatc
    27901 gtcgggtggt gccggatgcc ggtaacctgc tggcgcaaca ggcgattgcc gatgtgttct
    27961 gtgtcaacgg cgacagcgaa tggcgcggct taggcgtgat tgaatcttct ggtgtacacc
    28021 tgacgccgga ttatcaacga ttcgatgccg aagcgcattt ccgcccggca ccgcagcagg
    28081 tctgcgatga cccgcgcgca cgttgtggcg aagtattaac gggcaaatgt aagccgcatc
    28141 aatgcccgct gtttggtaac acctgtaatc ctcaaaccgc gtttggtgcg ctgatggttt
    28201 cctccgaagg agcgtgcgcc gcgtggtatc agtatcgtca gcaggagagt gaagcgtgaa
    28261 taatatccaa ctcgcccacg gtagcggcgg ccaggcgatg cagcaattaa tcaacagcct
    28321 gtttatggaa gcctttgcca acccgtggct ggcagagcag gaagatcagg cacgtcttga
    28381 tctggcgcag ctggtagcgg aaggcgaccg tctggcgttc tccaccgaca gttacgtcat
    28441 tgacccgctg ttctttcctg gcggtaatat cggcaagctg gcgatttgcg gaaccgcgaa
    28501 tgacgttgcg gtcagtggcg ctattccgcg ctatctctcc tgtggcttta tcctcgaaga
    28561 aggattgccg atggagacac tgaaagccgt agtgaccagc atggcagaaa ccgcccgcgc
    28621 ggcaggcatt gccatcgtca ctggcgatac taaagtggtg cagcgcggcg cggcagataa
    28681 actgtttatc aacaccgctg gcatgggcgc aattccggcg aatattcact ggggcgcaca
    28741 gacgctaacc gcaggcgatg tattgctggt tagcggtaca ctcggcgacc acggggcgac
    28801 tatccttaac ctgcgtgagc agctggggct ggatggcgaa ctggtcagcg actgtgcggt
    28861 actaacgccg cttattcaga cgctgcgtga cattcccggc gtgaaagcgc tgcgtgatgc
    28921 cacccgtggt ggtgtgaacg cggtagttca tgagttcgcg gcagcctgcg gttgcggtat
    28981 tgaaatttca gaagcggcac tgccggttaa acctgctgtg cgcggcgttt gcgaactgct
    29041 gggactggac gcccttaact ttgccaacga aggcaaactg gtgatcgccg ttgaacgcaa
    29101 cgcggcagag caagttttgg cagcgttaca ttcccatcca ctggggaaag acgcggcgct
    29161 gattggtgaa gtggtggaac gtaaaggtgt tcgtcttgcc ggtctgtatg gcgtgaaacg
    29221 aaccctcgat ttaccacacg ccgaaccgct tccgcgtata tgctaataaa attctaaatc
    29281 tcctatagtt agtcaatgac cttttgcacc gctttgcggt gctttcctgg aagaacaaaa
    29341 tgtcatatac accgatgagt gatctcggac aacaagggtt gttcgacatc actcggacac
    29401 tattgcagca gcccgatctg gcctcgctgt gtgaggctct ttcgcaactg gtaaagcgtt
    29461 ctgcgctcgc cgacaacgcg gctattgtgt tgtggcaagc gcagactcaa cgtgcgtctt
    29521 attacgcatc gcgtgaaaaa gacaccccca ttaaatatga agacgaaact gttctggcac
    29581 acggtccggt acgcagcatt ttgtcgcgcc ctgatacgct gcattgcagt tacgaagaat
    29641 tttgtgaaac ctggccgcag ctggccgcag gtgggctata ccccaaattt ggtcactatt
    29701 gcctgatgcc actggcggcg gaagggcata tttttggtgg ctgtgaattt attcgttatg
    29761 acgatcgccc ctggagcgaa aaagagttca atcgtctgca aacatttacg cagatcgttt
    29821 ctgtcgtcac cgaacaaatc cagagccgcg tcgtcaataa tgtcgactat gagttgttat
    29881 gccgggagcg cgataacttc cgcatcctgg ttgccattac caacgcggtg ctttcccgcc
    29941 tggatatgga cgaactggtc agcgaagtcg ccaaagaaat ccattactac ttcgacattg
    30001 acgatatcag catcgtctta cgcagccacc gtaaaaacaa actcaacatc tactccactc
    30061 actatcttga taaacagcat cccgcccacg aacagagcga agtcgatgaa gccggaaccc
    30121 tcaccgaacg cgtgttcaaa agcaaagaga tgctgctgat taatctccac gagcgggacg
    30181 atttagcccc ctatgaacgc atgttgttcg acacctgggg caaccagatt caaaccttgt
    30241 gcctgttacc gctgatgtct ggcgacacca tgctgggcgt gctgaaactg gcgcaatgcg
    30301 aagagaaagt gtttaccact accaatctga atttactgcg ccagattgcc gaacgtgtgg
    30361 caatcgctgt cgataacgcc ctcgcctatc aggaaatcca tcgtctgaaa gaacggctgg
    30421 ttgatgaaaa cctcgccctg accgagcagc tcaacaatgt tgatagtgaa tttggcgaga
    30481 ttattggccg cagcgaagcc atgtacagcg tacttaaaca agttgaaatg gtggcgcaaa
    30541 gcgacagtac cgtgctgatt ctcggtgaaa ctggtacagg taaagagctg attgcccgcg
    30601 cgatccataa tctcagtggg cgtaataatc gccgcatggt aaaaatgaac tgcgcggcaa
    30661 tgcctgccgg attgctggag agcgatctgt ttggtcatga gcgtggggct tttaccggtg
    30721 ccagcgccca gcgcattggt cgttttgaac tggcggataa aagctccctg ttcctcgacg
    30781 aagtgggcga tatgccactg gagttacagc cgaagttgct gcgtgtatta caggaacagg
    30841 agtttgaacg cctcggcagc aacaaaatca ttcagacgga cgtacgttta attgccgcga
    30901 ctaaccgcga tctgaaaaaa atggtcgccg accgtgagtt ccgtagcgat ctctataacc
    30961 gcctgaatgt attcccgatc cacctgccgc cactacgcga gcgtccggaa gatattccgc
    31021 tgctggcgaa agcctttacc ttcaaaattg cccgtcgtct cgggcgcaat atcgacagca
    31081 ttcctgccga gacgctgcgc accttgagca acatggagtg gccgggtaac gtacgcgaac
    31141 tggaaaacgt cattgagcgc gcggtattgc taacacgcgg caacgtgctg cagctgtcac
    31201 tgccagatat tgctttaccg gagcctgaaa cgccgcctgc cgcaacggtt gtcgcccagg
    31261 agggcgaaga tgaatatcag ttgattgttc gcgtgctgaa agaaaccaac ggcgtggttg
    31321 ccgggcctaa aggcgctgcg caacgtctgg ggctgaaacg cacgaccctg ctgtcacgga
    31381 tgaagcgact gggaattgat aaatcggcat tgatttaaca gatgtggtta tactcgatca
    31441 acgttcaatg aaaggaggca gtatggcagt ttcagcaaga aatcaattaa ccggaacagt
    31501 tagcgcagta gcaatgggcg ctgtgaatga tgaagtagaa ctgacgctgg caggtggtgc
    31561 aaagctggtg gcaattgtga ctcacagcag ccagcaagcg ctgggtctgg cgaaagggaa
    31621 agaagcgatt gccctgatta aagccccgtg ggtaacgctg gcaacggaag actgtggact
    31681 gaaattctct gctcgcaacc agtttgctgg cagcgtgtcg acaatcaccg aaggcgcagt
    31741 gaacgccact gtccatatca aaacggatgc gggttttgaa attgtggcag tggtaacaaa
    31801 tgaaagtcag gatgaaatga aactcaccac tggctcgcgc gttattgcat tgatcaaagc
    31861 atcagccatc ctgattgcca ccaaagcgta agcgccttat ttgccggaga gtatggctct
    31921 ccggcagatt tatttctttg gacgatattt ttctggcaac tccggcaccg gacgcttgtc
    31981 atcgatgaga tgacgcacgg ttaagatcgg atgacgccac agcattcgcg gcccagccca
    32041 acgcataatc tgtttcatct cttcacgctt tgcaggctgg taacagtgca ccggacactg
    32101 cttacaggct ggtttctctt cgccgaacac acatttatcc agccgctttt gcgcgtaagt
    32161 aaacaacgtc tcataatgct ccggctccgc tgacgcctgc gggcatttcg cttgataaag
    32221 atcgatcatt tttttaatcg tcagtttttc acgagagata cgcttgccgg acatgctgcc
    32281 tccatctcat taagatgcat ttatattaca acttaatctt aaagggcact atgactctaa
    32341 agaagaaggg ttagccaacc gatacaattt tgcgtacttg cttcataagc atcacgcaaa
    32401 agctgcaaaa cagcatcttt cccggaacca gcatcaagaa ctcgccgttc gcttcttccc
    32461 ctgaaatgat taactccggt atcatgtgcg ccttatgtga ttacaacgaa aataaaaacc
    32521 atcacacccc atttaatatc agggaaccgg acataacccc atgagtgcaa tagaaaattt
    32581 cgatgcccat acgcccatga tgcagcagta tctcaagctg aaagcccagc atcccgagat
    32641 cctgctgttc tatcggatgg gtgactttta cgaactgttt tatgacgacg cgaaacgcgc
    32701 ctcgcagctg ctggatattt cactgaccaa acgcggtgct tccgcgggag agccgatccc
    32761 gatggcgggg atcccctacc atgcggtgga aaactacctc gccaaactgg tgaatcaggg
    32821 cgagtccgtt gccatctgcg aacaaattgg cgatccggcg accagcaaag gtccggttga
    32881 gcgcaaagtt gtgcgtatcg ttacgccagg caccatcagc gatgaagccc tgttgcagga
    32941 gcgtcaggac aacctgctgg cggctatctg gcaggacagc aaaggtttcg gctacgcgac
    33001 gctggatatc agttccggtc gttttcgcct gagcgaaccg gctgaccgcg aaacgatggc
    33061 ggcagaactg caacgcacta atccggcgga actgctgtat gcagaagatt ttgctgaaat
    33121 gtcgttaatt gaaggccgtc gcggcctgcg ccgtcgcccg ctgtgggagt ttgaaatcga
    33181 caccgcgcgc cagcagttga atctgcaatt tggcacccgc gatctggtcg gttttggcgt
    33241 cgagaacgcg ccgcgcggac tttgtgctgc cggttgtctg ttgcagtatg cgaaagatac
    33301 ccaacgtacg accctgccgc atattcgttc tatcaccatg gaacgtgagc aggacagcat
    33361 cattatggat gccgcgacac gtcgtaacct ggaaattact cagaacctgg cgggcggtgc
    33421 ggaaaacacg ctggcttcag tgctcgactg cactgtaacg ccaatgggta gtcgtatgct
    33481 gaaacgctgg ctgcatatgc cagtgcgcga tacccgcgtg ttgcttgagc gccagcaaac
    33541 tattggcgca ttgcaggatt tcaccgccga gctacagccg gtgctgcgtc aggtcggcga
    33601 cctggaacgt attctggcac gtctggcttt acgaactgct cgcccacgcg atctggcccg
    33661 tatgcgtcac gctttccagc aactgccgga gctgcgtgcg cagttagaaa atgtcgatag
    33721 cgctccggta caggcgctgc gtgagaagat gggtgagttt gccgaactgc gcgatctgct
    33781 ggagcgggcc attatcgaca caccgccggt actggtgcgc gacggtggtg ttatcgcatc
    33841 gggctataac gaagagctgg atgagtggcg cgcgctggct gacggcgcga ccgattatct
    33901 ggagcgtctg gaagtccgcg agcgtgaacg taccggcctg gacacgctga aagtcggatt
    33961 taacgcggtg catggttatt acattcagat cagccgtgga caaagccatc tggcaccgat
    34021 taactacatg cgccgccaga cgctgaaaaa cgccgaacgc tacattattc cagagctaaa
    34081 agagtacgaa gacaaagttc tcacttcaaa aggcaaagca ctggcactgg aaaaacagct
    34141 ttatgaagag ttgttcgacc tgctgttgcc gcatctggaa gcgttgcaac agagcgcgag
    34201 cgcgctggcg gaactcgacg tgctggtgaa cctggcagaa cgggcctata cgctgaacta
    34261 cacctgcccg acctttattg ataaaccggg tattcgcatc accgaaggcc gccatccggt
    34321 ggttgaacag gtgctgaatg agccgtttat cgccaacccg ctgaacctgt cgccgcagcg
    34381 tcgcatgttg atcattaccg gtccgaatat gggcggtaaa agtacctata tgcgccagac
    34441 cgcactgatt gcgctgatgg cctacatcgg cagctatgta ccggcacaaa aagtcgagat
    34501 tggacctatc gatcgcatct ttacccgcgt aggggcagcg gatgatctgg catccgggcg
    34561 ttcaaccttt atggtggaaa tgaccgaaac cgccaatatt ttacataacg ccaccgagta
    34621 cagtctggtg ctgatggatg agattgggcg cgggacgtcc acttacgatg gtctgtcgct
    34681 ggcgtgggcg tgcgcggaaa atctggcgaa taagattaaa gcgttgacac tgtttgccac
    34741 ccactatttc gagctgaccc agttaccgga gaaaatggaa ggcgtcgcca acgtgcatct
    34801 cgatgcgctg gagcacggcg acaccattgc ctttatgcac agcgtgcagg atggcgcggc
    34861 gagcaaaagc tacggcctgg cggttgcagc tctggccggc gtgccaaaag aggttattaa
    34921 gcgcgcacgg caaaaactgc gtgagttgga aagcatttcg ccgaacgccg ccgctacgca
    34981 agtggatggt acgcaaatgt ctttgctgtc agtgccagaa gaaacgtcgc ctgcggtcga
    35041 agcactggaa aatctcgacc cggattcact gactccgcgt caggcgctgg agtggattta
    35101 tcgcttgaag agtctggtgt aataacaatt cccgatagtc ttttgctatc gggaatatta
    35161 acgacaacag acgaatcaaa taaaaacacc ctgtataata ggaaatctta ttttacaggg
    35221 taaaaccatg ccatctacac gctatcaaaa aattaatgcc catcactatc gttatatatg
    35281 ggccgttggt gatattcatg gtgactatca attattacaa tcccgcttac atcaactctc
    35341 ttttttccct gaaaccgact tacttatttc tgtcggcgat aatattgatc gtgggccgga
    35401 gagtcttaac gtactacgcc tgttaaacca gccctggttt acatcggtta aaggcaacca
    35461 tgaagcgatg gcgcttgatg cattcgaaac tggcgatgga aatatgtggc ttgccagcgg
    35521 cggcgactgg ttctttgaat taaatgagac ggaacaacaa gaagcaataa atctgttgct
    35581 gaaatttcat cgccttccac atattattga aatcactaac gacataataa aatatgtcat
    35641 cgcacatgca gattatccgg ggaatgaata tctctttggc aaagaaatag cggagagaga
    35701 actactctgg cctgttgatc gtgtgcagaa atcgcttaat ggcgagttac aacaaataaa
    35761 cggcgctgat tattttatat ttggacatat gatgtttgat aacattcaga cgttcgcaaa
    35821 ccagatttat attgataccg gatcgccgaa aagcgggcgg ctgtcatttt ataaaataaa
    35881 gtagtctcat gcttcttctg tgaagcatga gtaacccggt gttattgcag gccattattc
    35941 atttttcgct accagcaaag agagatcctg cttcgccagc gcgcgactgg cactctccgg
    36001 caaaccgtcg tctgtaataa tctgatcaaa ctcgcttaat ggtaacgcca gccatgtcgc
    36061 cacctgacca tatttcgtcg catcacagac caaaactcgc tggcggctgg cactggcaat
    36121 cgcccgtttc accgtgactt tatcttccgc tggcgtagaa atcccccgca cactccatga
    36181 cgatgcagaa ataaaagcct gatcaatcat caggctgcgc agcatggtcg cagcggcttc
    36241 cccgacacag gaacggtttt cccgacacac tgcaccgcca gtgtgaataa ttgtgcaatt
    36301 actgttgtcg agcaagtagt ccgcaataac gaaatcgttt gtgaccacag tcagtgactc
    36361 catgtgaatc agatgctgtg ctatcgctag cgtggtcgtt cccgcatcca gatagataca
    36421 acttcccggc tgaacaagac ttgccgccag cttgccaata gccgcttttt gcgtcattgc
    36481 cagcgcagtt tttacctgat gagaaggttc atgcgccacg cgtcccggag actggacgcc
    36541 tccggacacc agcacaacgg ctccctgctg ctccagtttt tgtaaatccc gacgaatggt
    36601 catatgtgac acattcattc tgtccgttag ttcagcaata ctgacaatgc ctttttcagc
    36661 taccatctca aggatgattt ggcgacgctc tacgggtatc aacttttgct ccttcctttg
    36721 tcctgctgac attctacgct atttgcctgc gaaacgtgcg cggcgcaact aacgcttagt
    36781 tcacataaaa taacacacaa tgttaatttt tgtgaatcag ctcaccatat cgttatcttc
    36841 cagcgcctat attcacaata tcaaacaaaa tatcacttaa attaacaagg agagcagatg
    36901 aaaacgggat ctgagtttca tgtcggtatc gttggcttag ggtcaatggg aatgggagca
    36961 gcactgtcat gtgtccgcgc aggtctttct acctggggcg cagacctgaa cagcaatgcc
    37021 tgcgctacgt tgaaagaggc aggtgcttgc ggggtttctg ataacgccgc gacgtttgcc
    37081 gaaaaactgg acgctctgct ggtgctggtg gtcaatgcgg cccaggttaa acaggtgctg
    37141 tttggtgaaa caggcgttgc acaacatctg aaacccggta cggcagtaat ggtttcttcc
    37201 actatcgcta gtgctgatgc gcaagaaatt gctaccgctc tggctggatt cgatctggaa
    37261 atgctggatg cgccagtttc tggtggtgca gtaaaagccg ctaacggtga aatgactgtc
    37321 atggcctccg gtagcgatat tgcctttgaa cgactggcac ccgtgctgga agccgttgcc
    37381 ggaaaagttt atcgcatagg tgcagaaccg ggactaggtt cgaccgtaaa aattattcac
    37441 cagttgttag cgggcgtaca tattgctgcc ggagccgaag cgatggcact tgcagcccgt
    37501 gcggggatcc cgctggatgt gatgtatgac gtcgtgacca atgccgccgg aaattcctgg
    37561 atgttcgaaa accggatgcg tcatgtggtg gatggcgatt acaccccgca ttcagccgtc
    37621 gatatttttg ttaaggatct tggtctggtt gccgatacag ccaaagccct gcacttcccg
    37681 ctgccattgg cctcaacagc attgaatatg ttcaccagcg ccagtaacgc gggttacggg
    37741 aaagaagacg atagcgcagt tatcaagatt ttctctggca tcactctacc gggagcgaaa
    37801 tcatgatcaa gattggcgtt atcgccgatg attttaccgg cgcgacggat atcgccagtt
    37861 ttctggtgga aaacggtcta ccaacggtac aaattaacgg tgttccaaca ggtaaaatgc
    37921 cggaagcaat cgacgcactg gtgatcagcc tgaaaacgcg ttcctgtccg gtggttgaag
    37981 ccacacagca atcgctggcg gcactgagct ggttgcaaca gcaaggttgc aaacagatct
    38041 atttcaaata ctgctctact ttcgacagtt cggcgaaagg taatatcggc ccggttaccg
    38101 atgcattaat ggatgctctc gacacgccgt ttacggtctt ctctccggcc ctgccggtca
    38161 acggacgtac ggtttatcag gggtatttgt tcgtaatgaa tcaactgctg gccgaatccg
    38221 ggatgcgcca tcacccggta aatcccatga ccgacagcta tcttccccgt ctggttgaag
    38281 cgcaatccac agggcgctgc ggcgtcgttt cggcacatgt tttcgaacaa ggtgtggatg
    38341 ccgttcgtca agagctggct cgcttacagc aagagggcta ccgctacgcg gtgcttgatg
    38401 cgctgaccga acaccatctg gaaattcagg gagaagcctt gcgcgatacc ccactggtaa
    38461 cgggcggttc tggtctggcg attggccttg cccggcagtg ggcgcaagaa aacggtaacc
    38521 aggctcgcaa agcagggcat ccgctcgctg ggcgcggcgt agtgctctcc ggttcatgct
    38581 ctcaaatgac caaccgccag gtagcacatt accgtcaaat tgcaccagcc cgtgaagttg
    38641 atgtggcacg ctgcctctca attgaaactc tggccgctta tgcacacgaa ctggcagagt
    38701 gggttctggg ccaggaaagt gtacttgctc cactggtttt tgccaccgcc agcactgacg
    38761 cattggcagc aattcaacag caatacggtg cacaaaaagc cagtcaggca gtagaaacac
    38821 tgttttctca actagcggcg cggttagcag cggaaggcgt gacacgcttt attgtcgcag
    38881 gcggtgagac ctccggcgta gtcacacaga gcctgggaat aaaagggttt catattggcc
    38941 caaccatttc tcccggcgtg ccgtgggtaa acgcactgga taaacctgtc tcactcgccc
    39001 ttaaatctgg caacttcggt gatgaagcct ttttttcacg agcccaaaga gagtttttat
    39061 catgagcgat ttcgcaaaag tagagcagtc tttgcgagag gagatgacgc ggattgccag
    39121 ttcattcttt cagcgcggct atgcaaccgg ttcggctggc aatctgtcgc tgcttttacc
    39181 tgacgggaat ttactggcga caccgacagg ttcatgcctg ggcaatctcg atccgcagcg
    39241 gctttccaaa gtcgccgcgg atggcgaatg gttaagtggt gacaaaccct cgaaagaggt
    39301 gctctttcat ctggcgctgt atcgcaacaa tccgcgctgt aaagcggtgg tgcatttgca
    39361 cagcacatgg tcgacggcgc tttcctgcct gcaagggctg gacagcagca acgttattcg
    39421 tccgttcaca ccatacgtgg tgatgcggat gggaaatgtc ccgctggtgc cttattaccg
    39481 accgggcgat aaacgcatcg cacaggatct ggcggaactg gcagcagaca atcaggcttt
    39541 tttactggca aatcatggcc cagtggtttg cggtgaaagc ctgcaagaag ccgccaacaa
    39601 tatggaagag ctggaggaaa cggcaaagct gatttttatt ctcggtgacc gcccgatccg
    39661 ttatctgacc gcaggtgaaa ttgcggaatt aaggagttaa tgcaatgcct cgttttgcag
    39721 ctaatttatc catgatgttc accgaagtgc cttttattga acgcttcgcc gcagcgcgaa
    39781 aagccgggtt cgatgctgtg gaatttctgt ttccctatga ctactccacc ctgcaaatcc
    39841 aaaagcaact ggagcaaaac catctgacac tggcgctgtt caataccgct cccggagata
    39901 ttaatgcggg ggaatgggga ttatccgccc ttcccggacg tgagcacgaa gcacacgcag
    39961 acatcgacct ggcactggaa tatgcattgg cgctcaattg tgaacaagtc catgtgatgg
    40021 caggcgtcgt gcccgctggc gaagatgctg aacggtacag ggcagtattt atcgataatc
    40081 tccgctacgc tgccgaccgc tttgcgccac acggcaagcg aatattagtt gaagcactca
    40141 gccccggcgt taagcctcat tatctcttct ccagccagta tcaggcactg gctattgttg
    40201 aagaagttgc acgagataac gtgtttattc aactagatac ttttcatgcg caaaaagtag
    40261 atggcaacct gacgcattta atccgcgact acgccgggaa atatgcgcac gtacaaattg
    40321 ccggactacc tgatcgacat gaaccggacg atggagaaat caactatccg tggctgttcc
    40381 gcctgttcga tgaggtggga tatcagggct ggatcggttg tgaatataaa cctcgtggcc
    40441 tcaccgaaga agggcttggc tggtttgacg cctggcgcta attcgttaaa tccctaatta
    40501 caaagtaccc atacatcccc ccatctacgg atggggtagg ttaactataa ctttcagaca
    40561 gggtttccca tgtccacaat tacattgtta tgcattgcgt taactggcgt aatcatgctg
    40621 ttgctgctgg tcatcaaggc aaaggtacaa ccattcgttg ctctgctcct cgtcagcctg
    40681 ttagtcgcac ttgcggcggg tataccggcg ggcgaagtgg gtaaagtgat gatcgccggg
    40741 atgggcggtg tgcttggttc cgtcactatt attattggtc tgggcgctat gctggggcgt
    40801 atgatcgaac actctggtgg tgcagagtca ctggctaatt atttcagtcg caagttaggt
    40861 gacaaacgaa ctatcgctgc gctgactctg gcagcgttct tcctcggtat tcccgtcttc
    40921 tttgatgtcg gctttattat tcttgcgcca atcatttacg gttttgccaa ggttgccaaa
    40981 atatcgccac tcaaatttgg cctgcctgtc gctggaatca tgctaactgt tcatgtggcg
    41041 gtaccgccgc atccaggccc tgtcgccgca gcggggttac tccacgcaga catcggctgg
    41101 ctaaccatca tcggtattgc gatttctatt cccgtaggga ttgttggcta ctttgcagcg
    41161 aaaataatca ataagcgcca atatgcgatg tcggtggaag tactggaaca gatgcaactg
    41221 gctccggcca gtgaggaagg cgcgacaaaa ttaagcgata aaataaatcc accgggcgtc
    41281 gcgctggtca cctcgctaat tgttattcct atcgcgatta tcatggcggg tacggtttcc
    41341 gcaacactga tgccgccttc gcatcccctg cttggtacgc tacagctgat cggctcacca
    41401 atggtagctc taatgattgc gctggtgctg gcattctggt tattggcttt acgtcgcggc
    41461 tggagcttac aacacaccag cgacattatg ggctcagccc ttcccaccgc ggcggtagtg
    41521 attttggtta ctggtgctgg aggggtattt ggcaaagtgc tggtggaatc gggcgtcggc
    41581 aaagcccttg ccaacatgct acaaatgatt gacctgcctc tgttaccagc cgcatttatt
    41641 atttcactgg cgctgcgtgc atcgcagggg tcagcaaccg tagcaatcct gacaaccggc
    41701 gggttactct cagaagcggt gatgggattg aatccgattc agtgcgtatt ggtgacgctg
    41761 gcagcctgct ttggtgggct tggcgcgtca catattaatg actcagggtt ctggattgtg
    41821 accaaatatc tggggttatc ggtagcagac ggtctgaaaa cctggacagt gttaacgacc
    41881 attctcggtt ttaccggatt cttaattacc tggtgcgtat gggcggtaat ttgaccttaa
    41941 taaaaaggtc cgatgggcat cggacctttt atttagcgct taccttccgc cagcagcggc
    42001 gggatgctcg gcaccatcgg cgcgtcatca atatctttct gtgtcatgcg gaacgcttcg
    42061 ggataatgtt cgcggctggt gcggcgtagc ggttcggtat cgcgccaggt atagaggcaa
    42121 tgctggcact ggtagaccgt ccagacatct tttaccggcg atttcgccat cacttcaatc
    42181 tgttcatcag cacaacgtgg acaaatcatc ttgttctcct tatttacgtg cggccagcat
    42241 agcggtcagt ttttcagccc aggctttggt ttccggtaaa tccaccaccg gctggctgta
    42301 gtgaccacgg ttgtccgggg cgacaggcgt ggtggcgtcg ataatcagct tgtcggtgat
    42361 accggccggg cttgagccgg ggtcgagttc cagcacggac atattcggca actgcaccaa
    42421 atcccctgcc gggttcactt tcgaagagag cgcccacatc acctgcggca ggttgaacgg
    42481 atcaacgtct tcatcgacca taatcaccat cttcacgtag cccagaccgt gtggcgtggt
    42541 catcgcacgc aggcccaccg cgcgggcaaa gccaccgtag cgttttttgg tggagataat
    42601 cgccagcagg ccgtgggtgt acatggcatt taccgcctgc acttccggga actcggcttt
    42661 cagttgctga tacagcggca cgcaggtggc tggccccatc aggtagtcga tttccgtcca
    42721 cggcatcccg agatagagcg attcaaaaat gggtttgctg cgataagaga ctttatcgat
    42781 acgcaccacg gtcatgttac gcccgccgga gtagtgcccg gtaaactcac caaacggccc
    42841 ttcgatttcg cgtttacggc tttcgatgac cccttcgagg atcacttctg aaccccacgg
    42901 cacatcaaaa ccagtcagcg gggcggtggc gatcgggtac gggctttcac gcagcgcgcc
    42961 tgccatttcg tactcggact gatcatattt cagcggcgtg gcccccatca gggtgatgat
    43021 cggatcgtta ccgagtgtga tggcaatcgg cagatcttca ccgcgttctt ccgctttatg
    43081 cagatgcagg gcgatatcgt gcatcggcac cggttgcaga ccgagcttac gcttaccctt
    43141 cacttccata cggtagatgc cgacgttctg cttgccgaag ttatccggat caagcggatc
    43201 gcgggaaacc acgcacgctt tgtcgagata gaaaccgcca tcaccgtcgt ttaaacgaaa
    43261 cagaggcaga atatcgaaca ggttgatctc gtcgccatcg acggtgttct gcgcccaggc
    43321 tggattggcg cggcgctccg gggcaatggg gaagttatcc cagcggcgga taaactcatc
    43381 aatctgtttt ttaaccgggg tgtttggcgg caggccgagg gaaatcgcgt ggttctgcca
    43441 ggaaccgatg gtgttcattg ccacgcgggc atcgataaag ccgcgaatat tgtcaaacca
    43501 cagcgccggt gcaacatcgc cgatacgtcc ggtggcgttg gcagcggctg ccagatccgg
    43561 ttccgcgttc acctcttcgc tgattttcag taactggccg tggtcatcaa gcgcctgtaa
    43621 aaagctgcgt aaatcatcaa atgccattat tcattctcct gagaaaaatt ccgggcctgc
    43681 ggcaatcctt gccagcgcct ggcgtgagga tgttcgaggc caaattgatc cagcacgcgg
    43741 gctaccacgt ggtggacaat gtcatctacc gtttcgggat ggttataaaa ggcaggcatc
    43801 ggcggcacca tcgccacgcc catgcgtgaa agtgcgagca tattttcgag atggatggtg
    43861 ctaagcggca tttcacgcgg caccagcact agtttgcggc cttctttgag cacgacgtcc
    43921 gccgcgcgcc ctaccaggcc atcggcgtaa ccggcacgga taccggcgag cgttttcata
    43981 ctgcacggaa taacgatcat gccgtcggta cgaaaggaac ctgaggagat ggttgcggcc
    44041 tgatccgccg ggttatggca gaagtctgcg agggcagcaa catcgcgggc gttgtaaggc
    44101 gtttccagtt caatggtggt tttcgcccac ttcgacatca ccagatgagt ctcgacattc
    44161 ggcatctccc gcagcgcttg cagtaatgcc acaccaagag gcgctccggt ggcccctgtc
    44221 atcccgacga tcagtctcat tgtttattct cttaattgtt cgtatacgaa cgttattatt
    44281 aaaatacgcc tctgttttca tcgcggcaag cactgaaaag taacaacatc ttcttcgtca
    44341 ctgaaaaacc gctatgatag cggcagatag tttgcccgga gtttacatgg cgttacgaaa
    44401 taaagcgttc catcagttac gacagctttt tcagcagcac acggctcgct ggcagcacga
    44461 gttacctgac ctcaccaaac cacagtacgc ggtgatgcgc gccattgctg ataagcctgg
    44521 tatcgaacag gtggcactga tagaagcagc agtcagcacc aaagcaacgc tggcagaaat
    44581 gttggcaaga atggaaaatc gtggcttagt cagacgtgaa catgacgccg ctgacaagcg
    44641 gcgacgcttt gtctggctga ctgccgaagg agaaaaggta cttgctgcgg cgatccccat
    44701 tggtgacagc gtggatgagg aatttttggg gcgtttgagt gcagaagagc aggagctgtt
    44761 tatgcagttg gtgcgcaaga tgatgaacac atagggtgca aattgccgga tgcgacgcta
    44821 tcgcggctta tccagcccac aaaaacacat gcttcccaaa caaaagaaaa aaggccagcc
    44881 tcgcttgaga ctggcctttc tgacagatgc ttacttactc gcggaacagc gcttcgatat
    44941 tcagcccctg cgtttgcagg atttcgcgca aacggcgcag gccttcaacc tgaatctggc
    45001 gaacacgttc acgggtgagg ccaatttcac gacctacatc ttccagtgtt gccgcttcgt
    45061 accccagcaa accgaatcga cgtgccagta cttcacgctg tttggcgttc agctcgaaca
    45121 gccatttgac gatgctctgc ttcatatcgt catcttgcgt ggtatcttcc ggaccgttct
    45181 ctttttcatc ggccaggatg tccagcaacg ctttttcgga atcaccaccc agcggggtgt
    45241 ctaccgaggt aatgcgctcg ttaagacgaa gcatacggct gacgtcatca actggcttat
    45301 ccagttgctc tgcgatctct tccgcacttg gttcgtggtc cagtttatgg gacaactcgc
    45361 gtgcggttcg caggtaaacg ttcagctcct ttacgatgtg aatcggcaaa cgaatagtac
    45421 gggtttggtt cataatcgcc cgttcgatcg tctggcgaat ccaccaggtt gcgtatgttg
    45481 agaagcggaa accacgttcc gggtcaaact tctctaccgc gcggatcagc cccaggttgc
    45541 cctcttcgat caggtccagc aacgccagac cacgattgcc ataacggcgg gcaattttta
    45601 ccaccagacg caagttactc tcgatcatcc ggcggcggga ggcaacatct ccacgcagtg
    45661 cgcgacgcgc aaaataaact tcttcttcgg ccgttaacag tggtgaataa ccaatctcac
    45721 caaggtaaag ctgagtcgcg tccaacacac gctgtgtggc tccctgcgat aacagttcct
    45781 cttcggccaa atcgttatca ctgggttcct cttctactaa ggccttttcg tcaaaaacct
    45841 caactccgtt ctcatcaaat tccgcatctt catttaaatc atgaactttc agcgtattct
    45901 gactcataag gtggctccta cccgtgatcc cttgacggaa cattcaagca aaagcctggt
    45961 tccgccgatt tatcgctgcg gcaaataacg cagcgggttt acggatttcc ccttgtaacg
    46021 aatttcaaaa tgcaagcgtg ttgaactggt tccggtgcta cccatggttg ctattttttg
    46081 ccccgccttc acttcttgtt gttcccggac cagcattgtg tcgttatggg cgtaggcact
    46141 caggtaatca tcattatgtt tgatgataat cagattaccg tagccgcgca gcgcgttacc
    46201 ggcataaaca acgcggccat ctgcggtcgc gataattgcc tgtcctttgc tgcccgcgat
    46261 atcaatcccc ttgttgcccc cctcagaagc gccaaaggtt tcgatcactt tgccctcagt
    46321 cggccagcgc caggtggaga taggcgtact ggttgatgta ctgctgacag tcggctcggt
    46381 tgtgcttgct gttggtaccg ttacaggcgc tgtgaccgtg gtcgcagttg gcttgttgtt
    46441 cggcaacatt ttgttagcac tctgttcacc cgaagactca gaatacgtaa ttgtcggttg
    46501 cgaagcaaca gcaacggtgg aattttgtgc aggcttgatc acaactcctt gctctgctgc
    46561 atcggcctgg gtaatggcat ttccgccagt gattggcgta ccggaagcat tacccacctg
    46621 taaggtctga ccgacgttca gcgcgtatgg tgcctgaata ttgttgcgct gagcaaggtc
    46681 acggaaatcg ttgccagtaa tccaggcgat atagaaaagt gtgtcgcctt ttttcacggt
    46741 ataggtactg ccgctataac tgcctttcgg aatgttccca tactgacggt tgtagacgat
    46801 gcgtccgttt tccatctgta ccggctgctg agctactggc tgtactggct ggatttgcgg
    46861 ttgttgagta gcctgaattt gtggctgctg taccggctga atttgcggtt gctgcgctgt
    46921 agacgtcgtt cccattttcg gcggcggcgt aatcaacata ccagaattgg tatttgcagg
    46981 cgcattgcca ttaacggagc tgaccggtgc cggtggattt gaagtgtcag aacagcctgc
    47041 cagccatagc gaaaccagtg acaaagccgc aatgcggcga acggtgaatt ttgggcttcc
    47101 cgcgctcatt tatcccccag gaaaaattgg ttaataacca gtgacataat taccgtgcaa
    47161 ggcaccatac tgaacactgg aaaagatgtt cacgatacgc tgacctgcgg caaaataacc
    47221 aggaaaaatc caggtatttc ctcacgtttt aagccagctc acccttcact aaagggacaa
    47281 agcgcacggc ctccacggta tcgataataa attcgcctcc ccgacgacgc acccgtttca
    47341 aatactggtg ctcctcccct acgggtaaga cgagaatccc gccttcgtcc agctgcgtca
    47401 ttagtgcagt tggaatttcc ggcggtgccg ccgtaacaat gatagcgtca aacggcgcac
    47461 gtgcctgcca accttgccat ccatcgccat gacgggttga aacattatgt aaatcaagat
    47521 ttttcaggcg gcgacgcgcc tgccactgca aacctttaat ccgttcgacc gagcaaacat
    47581 gctggacaag atgcgccagg attgccgttt gatatcccga accggtgcca atttccagca
    47641 cccgcgactg cggcgtcagc tcgagtaatt cggtcattcg cgccaccata tacggctgcg
    47701 aaattgtctg cccctgacct atcggcaagg cgatattgtc ccaggctttt tgttcaaacg
    47761 cttcatcaac gaatttttca cgcggcacgg cggcaagtgc attcagcacc tgctcatcct
    47821 gaataccttg cgcacgtaat tgatccagaa gtgcttgtac gcgtctgctt accattgcgt
    47881 gccaactccc acgctgttta accagtctga aaccacatct tgcgcgctat gcgcagttaa
    47941 atccacatgc agcggcgtga tggagacata gccctcatct accgcagcaa aatcggtccc
    48001 cggaccggca tcacatttac cgcccggcgg gccaatccag tacagcgtat tgccgcgcgg
    48061 atcttgctgc gggatcacct gatctgccgg atgtcgtgta ccgcagcgcg tcacgcgaat
    48121 acctttgatt tgatccaatg gtaaatccgg aacgttaata ttaagaatac gtccggtgcg
    48181 cagcggctct ttacacagtg cgcgcaaaat tgaacaggtt accgccgcgg cagtgtcgta
    48241 atgtttatgc ccgtcaagcg agacggcaag cgccggaaaa cctaaatgac ggccttccat
    48301 cgcggcggct accgtaccgg aataaataac atcatccccc agattcggcc cggcgttaat
    48361 tccggacaca acaatgtccg ggcgcggacg catcagagca ttcacgccaa gatagacgca
    48421 atcggtcggg gttcccattt gcacagcaat atcaccattt tcaaaggtaa acgtgcgcag
    48481 ggaggattcc agtgtcagag aatttgaagc gccgctgcgg ttacgatcgg gggcgaccac
    48541 ctgaacgtca gcaaactcac gcaaggcttt cgccagcgtt tgtataccgg gtgcatgtac
    48601 cccgtcatca ttactcagca atatgcgcat aatcacctgt tgtgttgata agttccctga
    48661 caacgctggt tgcaaaacta cccgccggaa gccagaaacg gatttctacg gtgacgtcat
    48721 cccaccagtt ccagcttaat tgttgcggat acagcagcat cgctctgcgc gcggcttcaa
    48781 ctttttcgcg caccagtaag gcttgtaatt cagtttctgc ggcgacagct gcttgttcga
    48841 atgccaacgc ttcacgctga gttccccatt cgccactgcc cggcaatgcg gcggttatca
    48901 tcagctcttt atcgttgacg cgacgctgta attccgccag ttcttcggtg gttgcgacaa
    48961 accagctacc acgtccggct aattgtagcg catcgccgtc aacaacttga ttaacgtctg
    49021 cttttttgag gcgctcagca acaatctgat taaacaacgc actgcgggct gccgacaacc
    49081 aaaaactccg tttattgcga tcgcgcaccg gagtattggt ttgcgcccag cgcagcgcgc
    49141 cctgcaagtt gctaccgcca atcccaaaac gttgggcacc aaaatagtta ggcacgcctt
    49201 ttacgcaaat atcgatcaga cgttgttcaa cgtcatcgcg attgctcact tcgcgcagaa
    49261 ccagggtaaa ggcgttacct ttcagcgcgc ctaaacgcag cttgcgcttg tgccgcgcat
    49321 actccagcac ctggcagcct tccagttgaa aggcgctcag atcgggcatt tccttgcccg
    49381 gcacgcgagc acataaccac tgttccgtta cagcatgttt gtctttttgc ccagcgaagc
    49441 tgacttcacg ggcatgaatt ttaaggaatt tcgccagtgc atccgccaca aaacgggtat
    49501 tgcagccgtt tttgaggatt ctaaccagaa tatgctcacc ttcaccatca ggctcaaagc
    49561 ccaaatcttc caccaccaca aagtcttccg gattggcttt cagcagcccg gtgccttgcg
    49621 gtttaccgtg gaggtaagtg agattatcaa actcaatcat tttgttgcct taatgagtag
    49681 cgccaccgct tcacaggcaa tcccttcccc acgtccggta aatccaagtt tttccgtagt
    49741 agtggctttc acgttaacat catccatatg gcagccgaga tcttcggcaa taaacacgcg
    49801 catttgtgga atgtgcggca acatcttcgg tgcctgagcg atgatagtga catcgacgtt
    49861 gcccagggta taacccttcg cctgaatacg acgccaggct tcgcgtagca gctcgcggct
    49921 gtcggcacct ttaaatgccg gatcggtatc cgggaacagt ttgccgatat cccccagcgc
    49981 cgccgcgcca agcaatgcat cggtcaacgc atggagcgcc acgtcgccat cagaatgcgc
    50041 cagcaatcct ttttcgtaag gaatgcgtac gccaccaatg ataattgggc cttcaccgcc
    50101 aaaggcgtgt acgtcaaaac cgtgtccaat tcgcattatg tattctcctg atggatggtt
    50161 cgggtgaggt aaaactcggc cagtgccaaa tcttccgggc gcgtgacttt aatgttatcc
    50221 gcgcggcctt cgaccaactg cggatgaaat ccgcaatatt ccagcgccga ggcttcgtcg
    50281 gtaatggtag cgccttcatt tagagcgcgc gtcaggcagt catgtaacag ctcacgaggg
    50341 aaaaattgcg gcgtcagcgc gtgccataag ccgttgcgat caacggtatg agcaatggca
    50401 tttttgcccg gttcggcacg tttcatcgta tcgcgcactg gtgcggcgag gatccccccc
    50461 gtgcggctgg tttcgctcaa cgccaacaat cgcgcgaggt catcctgatg cagacaagga
    50521 cgagctgcgt catgcaccaa tacccactgc gcgtcgccag cggctttcag acctgccagc
    50581 accgaatcgg cacgctcatc accgccatct acaacggtga tttgcggatg attcgccaga
    50641 ggaagttgtg caaaacggct atcgccagga cttatggcaa tgacgacatg tttcacccgg
    50701 ggatgcgcca gcagcgcatg caccgagtgt tcaagaatgg tttgattacc gattgagaga
    50761 tattgcttag gacattccgt ttgcattcga cggccaaatc cggccgccgg aaccacggcg
    50821 caaacatcca aatgagtggt tgccatgtta attcctgggc tgatttatcg attgttttgc
    50881 cccgcagact gtgcgcgctt cgacgcgtca ggcaccagac gataaaaagt ttcgcccggc
    50941 ctggtcatgc tgagttcatt acgcgcacgc tcttcgagcg cctcctggcc gccattgaga
    51001 tcgtcaattt cggcaaaaag ttgatcgttt cgcgctttaa gtttcgcgtt tgtagcttgc
    51061 tgtgccgcca catcatcatt gacgcgggta tagtcatgta taccgttctt accgaaccac
    51121 agcgaatact gtagccagac cagaatagcc agcaacagca gcgttagttt acccatcctg
    51181 ccccctgaaa aacggcatca tcatcccatg catccgaaga cgactctaca tcctctgttg
    51241 gggataccgc gacaacgcgg gcaaatgtac cacatttgtc cattgttacg tatacccagg
    51301 gcgtgcagaa cataatctca ttattagtta cggtttgaat tatgaacaga ggagacaaga
    51361 aagtacaaat tagcccagta gccacataaa cagtgcgcca aacataatgc ctactgtcac
    51421 cagggtgaaa acaatactgt agcgtagctt tccgtccatc aatgaatgca gcgcaatccc
    51481 caccactacc gcgacaggca tcagtgccag aaagaaaggc caggtgtaga taaagaagaa
    51541 cagcgtgtta gagccataaa tcaacatcgg catcgccagc gcaaataacc atgagataaa
    51601 accgatcacg gcaccaggca gtgaccatgt ggtttcttca tcctcagtaa ggctgtcgtt
    51661 atttgttagt gtaatgttat ggctattacg catatttgat cctgttactt tgacgaaccg
    51721 ggcatggaaa cccggtggtg tctcaggatc tgataatatc gttctgtctc aacagatcta
    51781 ataattgctg taccaaattt gttactaatt gttcaccatt gagatgaatt tctgccgatt
    51841 caggcgcttc gtaaacggaa tctattcccg taaagttacg cagttcaccg gcacgcgctt
    51901 tcttatataa gccttttgga tcgcgggctt cgcaaatcgc cagcggtgta tcgacaaaca
    51961 cttcgataaa gcgcccttct cctacgcgtt cgcgaaccat ctggcgttcg gcgcggtgtg
    52021 gcgagataaa tgcggtcagc accaccagtc cggcttcaac catcaaattc gccacttcac
    52081 cgacgcgacg gatattttct ttacgatcgg catcgctaaa accgagatcg ctgcataatc
    52141 cgtggcgaac attgtcgcca tccagcagat acgtactgac gccgagttta tgtaatgcct
    52201 cctccagcgc cccggcgacc gttgatttac cggacccgga gaggccggta aaccacagca
    52261 ctacaccacg atgaccgtgg tgtagctcgc gttgttgcac agtgaccgga tggctatgcc
    52321 agacgacgtt ttcgtcatgc agcgccatta tttatccccc agcaaatcgc gcgcgcccca
    52381 gtgcggaaag tggcgacgaa ccagtgcatt caattccagt tcgaatgcac tgaattcaga
    52441 tggcgcagta gttgcctggc taactggctc gtgcaccata ccggcaccta cggtcacatt
    52501 actcaggcga tcgataaaaa tcagcccacc ggtaaccggg ttttgctgat aacgatctaa
    52561 caccagtggc tcgtcaaagg tgagatccac caagccgatg ccgttcagcg gcaggttttc
    52621 aacttcgcgt tgggtaaggt tattaatatc aacctgataa cgaatgccat caacacgagc
    52681 acgcgtcttc ttaccggcaa ttttgatgtc gtaacgctgg cctggggaaa gcggctgttc
    52741 cgccatccat accacatcca ccgacgcgct ctgcacagcc ggtaatgatt cgtctgccgc
    52801 cagcagcaga tcgccacggc tgatgtcgat ctcatccgtc agcaccaggg tgatcgcttc
    52861 tccggcaaag gcttcttcgc gatcgccatc aaaagtcacg atccgcgcga cgtttgattc
    52921 cacaccagag ggcagcactt tgacacgttg cccgacttcc acgcgaccgg atgccagcgt
    52981 tccggcgtag ccacgaaaat cgagattcgg gcgattaacg tactgcaccg ggaagcgcat
    53041 tggctgggca tccaccactc gctggatctc cacggtttcc agcacttcga gcagtgtcgg
    53101 accgctgtac cacggcatac tttcactttg cgaagccacg ttgtcgcctt ccagtgcaga
    53161 gagcggcaca aagcggatat ccagattacc cggcagctgc ccggcaaacg tcagataatc
    53221 ttcgcgaata cgggtgaacg tctcttcact gtaacccacc agatccattt tgttgatcgc
    53281 cacgaccaga tgtttgatcc ccaacagtgt ggagataaaa ctgtgacgac gggtttgatc
    53341 gagcacgcct ttacgggcat cgatcagtaa gatcgccagt tcacatgtcg atgcgccagt
    53401 cgccatattg cgagtgtact gctcgtgccc tggggtatcg gcgataataa atttacgctt
    53461 ctcggtagag aaataacggt aagccacgtc aatggtgatg ccctgttcgc gctcagcttg
    53521 caggccgtcc accagcagtg ccagatccag tttttcgccc tgggtgccgt gacgtttact
    53581 gtcgttatgc agcgatgaga gctgatcttc gtagatttgg cgggtatcgt gcagcaggcg
    53641 accaatcagg gtacttttgc cgtcatcgac actaccacag gtcagaaaac gcagcaggct
    53701 tttatgttgt tgcgcaatca tccaggcttc gacgccgcct tcattggcga tttgttgtgc
    53761 aagtgcggtg ttcatcttaa aaatacccct gacgtttttt caactccatg gatcccgcct
    53821 ggtcgcggtc aatcacgcga ccctgacgtt cactggtggt ggaaaccagc atctcttcga
    53881 tgatttccgg cagtgtttgt gcatttgact ccaccgcacc ggtcagcggc cagcagccca
    53941 gcgtacggaa acgcaccatc cgttttttaa tcacttcgcc cggttgcagg tcgatacggt
    54001 tgtcatcaat catcatcaac ataccgtcgc gttccagaac cggtcgttcc gcagcgagat
    54061 acagcggaac aatgtcgata ttttccagcc agatgtattg ccagatatcc tgctcggtcc
    54121 agttagagag cgggaagacg cgaatgcttt cgcctttgtt aatttgcccg ttgtagttgt
    54181 gccacagctc cgggcgctga tttttcggat cccagcgatg gaagcggtca cggaaagagt
    54241 agattcgctc tttagcgcgg gatttctctt cgtcgcggcg tgcaccaccg aaggcggcat
    54301 caaaaccgta tttattcagc gcctgtttca ggccttcagt tttcataata tcggtatgtt
    54361 tcgcgctgcc atgaacgaat ggattaatcc ccatcgccac gccttccggg tttttatgca
    54421 ccagcagctc gcagccgtag gctttagccg tacgatcgcg gaactcatac atctcgcgga
    54481 atttccagcc ggtatcgaca tgcagcaacg ggaaaggcag cgtacctgga taaaacgcct
    54541 tgcgcgccag atgcagcatg acgctggaat ctttaccgat agagtagagc atcaccggat
    54601 ttgagaattc tgccgccacc tcgcgaataa tgtggatgct ttccgcctcc agttgccgca
    54661 ggtgagtaag tcgtatttga tccataaccg ttcctttgca ataccgctat tttcttgcca
    54721 tcagatgttt cgactatagg gagcgtaaga gaacgaatga aattaccaat tagaatgagt
    54781 agttccttaa cggaataacg atttggcaaa gctaatatca aaaagtgctt aaggcaccgg
    54841 atttcgggcg tttaggaaga tttgaaattg ttttagcgca gcggcagttt catactatgg
    54901 cggtaaaaaa atttgcatgg tatttaagga ctcactatgt tttccgcatt gcgccaccgt
    54961 accgctgccc tggcgctcgg cgtatgcttt attctccccg tacacgcctc gtcacctaaa
    55021 cctggcgatt ttgctaatac tcaggcacga catattgcta ctttctttcc gggacgcatg
    55081 accggaactc ctgcagaaat gttatctgcc gattatattc gccaacagtt tcagcaaatg
    55141 ggttatcgca gtgatattcg gacatttaat agtcggtata tttataccgc ccgcgataat
    55201 cgcaagagct ggcataacgt gacgggaagt acggtgattg ccgctcatga aggcaaagcg
    55261 ccgcagcaga tcatcattat ggcgcatctg gatacatacg ccccgctgag cgatgctgac
    55321 gccgatgcca atctcggcgg gctgacgtta caaggaatgg atgataacgc cgcaggttta
    55381 ggtgtcatgc tggaactggc agaacgcctg aaaaatacgc ctaccgagta tggtattcga
    55441 tttgtggcga ccagtggtga agaggaaggg aaattaggtg ctgagaattt actcaagcgg
    55501 atgagtgaca ccgaaaagaa aaatacgctg ctggtgatta atctcgataa cttaattgtt
    55561 ggcgataaat tgtatttcaa cagcggtgta aaaacccctg aagcagtaag gaaattaacg
    55621 cgcgacaggg cgctggcaat tgcgcgtagt catggaattg ccgcaacgac caatccgggt
    55681 ttgaataaaa attatccgaa gggtaccggg tgctgtaacg acgcggaagt ttttgacaaa
    55741 gccggtattg cggtactttc ggtggaggca actaactgga atcttgggaa taaggatggc
    55801 tatcagcaac gtgcaaaaac cgccgccttc cctgcgggaa caagctggca cgatgtcagg
    55861 cttgataatc aacaacatat tgataaggcg cttcctggaa gaatagaacg tcgctgccgt
    55921 gatgttatac ggataatgct accgctggtg aaagagctgg cgaaggcgtc ttgatgggtt
    55981 tgaaaatgag agctgggagt tctaccgcag gggcggggaa ctccaagtga tgtccatcat
    56041 cgcatccagt gcgtccggtt tatccccgct gacgcgggga acacggcttt aaccacacga
    56101 gcaagtcctt cgctggcggt ttatccccgc tggcgcgggg aacacagtta acaacccgcg
    56161 ccgaacaagc ccgacaacgg tttatccccg ctggcgcggg gaacactcgc accgcgttaa
    56221 tccggcagaa aaacaacacg gtttatcccc gctggcgcgg ggaacacccg acaatctccg
    56281 cctccagtcg gtcgacctgc ggtttatccc cgctggcgcg gggaacactg tgcccgactg
    56341 cgtgccgcgt cgagcgcctg cggtttatcc ccgctggcgc ggggaacact tcgccgccat
    56401 catcctgtgg tgccagttga ccggtttatc cccgctgacg cggggaacac gcgcctcggt
    56461 tgtttagcca tgctcagccc cacggtttat ccccgctgac gcggggaaca cctttccacc
    56521 caggcactga tactccacat aaacggttta tccccgctga cgcggggaac acgatcgccg
    56581 ggcgcggtta cccgctgcga aaatcggttt atccccgctg acgcggggaa cacctcacgc
    56641 gaggcatcga tcgtgatctg attgtcggtt tatccccgct gacgcgggga acacgatacc
    56701 ttgcgcttgt cgcaaaaaca gagccacggt ttatccccgc tgacgcgggg aacacatact
    56761 caaaacccaa ctcacctgtt gttttatcgg tttatccccg ctgacgcggg gaacacacta
    56821 agcatacata actgttttta aacaaattta ttccgcatca acaatctacc aactaaatta
    56881 aaacatttcc ttatttttaa agaacagata acttattgat tatcaacagg aagaaaagaa
    56941 accaaacgca ccccatccaa atccaccgga atacgtctat tttcacccca ggtctgaaat
    57001 tcaaaacccg actcggtatt ggtcgcccag gccattacca catttccgca acctgccagt
    57061 tgagagattt gctgccagat catctcccga atacgtttgg acgtatctcc gacataaaca
    57121 ccggcacgca cttccaggag ccagatcgcg agccgtccac gtaagcgcgg agggacattt
    57181 tctgtaacaa cgacgaccat actcatccgc cgcgtccccg gtgaccgcta tcgcccagcg
    57241 tttcaggttc agggatggca ggcggtaaca tatccggcgc gggttgtggt ggttcaattt
    57301 cacctgcggc aagcacttcc tcgattaacg gaattaattt gcccgttaac ttactgctac
    57361 ggaaaatatc acgacaggcc aacctgactt ctttatctgg ttctgcgggt tgcctcgctg
    57421 ctatttcaaa tgcctttggc acaaccgaat caaatttaat gatatcggct atgtcataaa
    57481 caaatgaaag cggtttgcca ctatggataa atccaatagc gggcgcatat cccgcggcta
    57541 atactgccgc ttcagaaata ccgtacagac atgatgtggc agcactgatg cagcgattca
    57601 caacatcgcc tttttcccag tctttaggat cgtatttgcg accattccat ttcacaccat
    57661 attgtttcgc cagtaatgca tacgtctggc gaacgcggga tccctcaatt ccacgtagct
    57721 gctccactga acggcgagct ggcggtggct cacgaaaacg taattcatac attttgcgca
    57781 ccaccttcag acgtagatct tccgttaaag ccagctttgc ctggtagagt aacttatcag
    57841 cacgcgcccc accgggttgt ccggaagagt agacgcgaac acccgcttca ccgacccaga
    57901 ccagtagcgt ccccaccgtg gcggccagat gcaccgccgc gtgggaaact ctcgttcccg
    57961 gttcgagcat aatgcaggcg accgatccca ccggaatgtg cgtgcggatc ccggttttgt
    58021 cgatcagcac gaaagcgccg tccagtacgt cgatttgacc gtactggagg aagatcatag
    58081 aggtgcgatc ttttaacggg atcggactca gtggtacaaa cgtcacgcct ctgctccggg
    58141 tttgatcagc atcagaccac agccgaacgc gcggcttttg ccatacccct ggctcagacg
    58201 ttgcagaaat aacccaggat cggtgaccgt aagcatcccc gtataatcca cgctactgaa
    58261 ctggatcagt tgccgggagt tttcccgccg cagttgctgt tgtcggtagg catcgaccga
    58321 ggtatccagt agtgtaaaac cgcttctttc cccctgcgct gccagccagt ccagcgccgc
    58381 ctgttgttga tgcaaccaga catcacttcc ttccacctgc cccctcacct gccgtttcgc
    58441 ctccatcagc aagtcgtggc gtttgcccgc tttacaaatt gttggattcg cccgcaggtt
    58501 gaaacaaagg gattgtccgg tacgcaactc tggcgcaaat ggtcggcatt cgatggtgaa
    58561 aatatcactt tccgccgggc gttcctgcga caggacaaaa aagcgaaacg ccccctggag
    58621 ttcttcccga cgataaagaa attgcctttc tttgccgcca ggaaaaagat cccacagcca
    58681 ctgatgcatt acatattcac cgcgatccac cagatgcagc aactgcgcag gcgaaagctg
    58741 gccggtatgt aaggttattc ttgagaggta catggttcct ccttgctgag ccacggcccc
    58801 tgattgatgg tgcgttcccc aaacagccac tgctgacgat ttaaaggaac atcccggcga
    58861 cgtaatattt tgctcgcgac caggccgtcg tgttcccctt cccaccagca ttcatcctga
    58921 agtttcggga gtgagacttt cagttcgcgg aaactatcct gatactgttg gtatgcgtta
    58981 cgtaagacat cagacgcgtt gccttcgagc agtaacggcg caagtggtaa cgccagcgga
    59041 tgactttttc gccccagata aagcggaaaa accggatgac gtaaaccgtc ctgcaactgt
    59101 tcaagcctgt aaggcgcatc gggggttgtt gccaccgcca ccatccacca ggcatcggtg
    59161 tagtagtcgc gccgggagat aatcgcgctc agaagatcag gggcgctcaa ctcttcgcga
    59221 cggctgaaat aacgcgcttt acgcacctct tttggcatct ggaccgtgtg ataatcccgt
    59281 gcccagcgcg ggttacggct ggcgcaaacc accagtgaat agtggctgtt aaacgcgttt
    59341 aatcgttcgg tatcatcacg ccgaatccct accccggcag ccagcagccc cagcaatgct
    59401 gagcgcgaag gcagttcatg ggtatgacgc acttcgccgg gggcatcgac gccccaggat
    59461 gccattggcc catgaagctg aaaaatcaaa tattggctca ttagccgccc cttacgcgca
    59521 gataaagtcc agcacgtcct tcatgcttcc ctgtttgttc atcacgtcaa agcttgcgca
    59581 ttcggtcttc tgttcataga ccgtattcat attttcgcga agcgttgtaa tacgctgcac
    59641 cgccacatct aactgccggg tgccattaat gggttcatag aaagccgccg ccagagagcg
    59701 tggttgttca gtgccttttt ctgccagcgc ccaggaggcg taggcacggc tggcaaagct
    59761 gttctgtttg ccggttgggg agactttaag tgcggcttcc gtaaaggcgc gcaaggtctg
    59821 attagctaac gcttcgtcac cgccgaggtt ttcgaccagc agatctttat cgatgcagat
    59881 ataggtgtag aacagcgcag aaccgaatcc ggtttcacca agatgcccgg caccggcatc
    59941 ttcagaagcc tggcgcaaat catcaacggc ggtgaaaaaa tcatcttcga caatcgtttc
    60001 actgacacca aatgcatgcg cgacctggca ggcggcttca acattaaatt cgggtttatt
    60061 cgccagcata cgaccaaaca tagcgatatc taccgccatg cgatctttac gtaacaaagc
    60121 gagatcttcc tcttttggcg cgcgcttttc ttcggccagt tgatgggcca gcgcttttac
    60181 ggcgtcaaat tctgccgggc tgatatggac tagctgttca gtttcggcgt tagtgagcgg
    60241 atcttttggt tttttgtcgt ttttagcttt cccaagataa tccgcaattt ttgccgccca
    60301 ttcgatggct tttttctctt cgatgccttt ctcaatcagg atagttgccg cctcacgcgc
    60361 aatacgccca ctgcgaatac caatatggcc cgccagtgcc tgttcaaaaa gtgcagaagt
    60421 gcgccacgca cgtttcagac tttgtgagga aacgcgcaga cgcgttgctc cacccagtac
    60481 cacagttttt ggcgcaccgg tgtcatcacg gttaaggttg gcagcagggt aagcggttaa
    60541 caaatgaagc tgaataaatg tcgtcatgag atagtccttt attaattttg ttcgttatcg
    60601 gcgtcgccag cctggtaata ttccagcgcc cagcggatac ggataaattc tgttggccgc
    60661 tgctggcggc ggtgatgatt gagcaggtcg tcgctctcct ggcaccagcg gaaaatatct
    60721 tctgccaaag agataagatt gacggaacca tttaataatt tgaccgcgcg acgtaactga
    60781 cgcagtaaat catcaggcgt ttttactgcc gacaggcgag taaaacgccc ctccgacatt
    60841 accgccgcca gctgcgcggc aaaaggctgc cgttcatcaa tagctttaac attggcgcta
    60901 actgcggcga ccagcgccag tgcggtaaaa cgccactctt gtgcttcaat tttccacaaa
    60961 gtgtgggtct gcatcagcag agaacgaaac ccttctgaca gacaaacatc attaaccgtt
    61021 gtgctacgcc gcagactggc acgattgccg cgtttttcct gcaactcttc gtgccattta
    61081 cgcagcgtgg ctttgtgctc ctcttttaca atactcattc agcagcctcc tgctttttct
    61141 ccctggcggc tttagcactt tgcttctccg ccgatgttgt aaaatatttc ttgcgcgcgg
    61201 tcatgacgcg tttcaaatca acgggctcat aaggattagt gaatacacgc tcgtcaaaat
    61261 cctgacgcgc gaataaccaa atttcctttt gccatttgcc gagtaattca tccgcatcct
    61321 gaccttcttc aatttggcgc actaacctca ggaagcgatg ctgagttttg ttccagaagt
    61381 cgatatccac aaaactgaaa tcaccccttg caccttttgg atcggagaac catgcttctt
    61441 tcaatgcact ccgtaacaga ctcagaatcc gtgaagccgt ttgcgcagcc agccgcagct
    61501 tcggtatctg gccttctttt ttattgagca gtagcgggaa atggtgttcg taccaacagc
    61561 gcgctttcat gttgtcaaaa tcataaccaa atccccatag gcccactttt gcctgtttca
    61621 gactgctggc attaaagagt ttcaccacca gcgcgggaag ttccgtattg ttttctgatt
    61681 tacccgtttc gataaggcct aaccagtcgc gccagattaa accgcccggt tgtggtttaa
    61741 cggagtaaaa ctcgcccccc tctttaagtg gtacacggta aggcgttaag ggatgctgcc
    61801 acatggcata attggcacca taatttttgg tcgtcatcag gctcaggagc gcgtcgttct
    61861 gctcgtcgca aatatcgcag ttgccgactg tcgtggtatt aaaatcaata cgtatacgcc
    61921 gcggcattcc ccagtacgcc tggagtttat tgacctgatc atgggttacc accgcaccgg
    61981 ccagttcgct ggtacgcgtc gggcctagcc aggggaaaat cagatcgtca aattttttgg
    62041 gtagcggtaa gtcggcttca tcctgcggca tcacgttgag ccatagtttg cgccacaagg
    62101 gagtttgttg attgccctga tattcctgta attcaatcag cgtggtcatc ggcccaccgc
    62161 cgcgtaagcc ggtgcgatag cctttgccac ctgacggcgc atttaactgt agagagaaca
    62221 aggctagtgc agaacactgt gggcacaggt gttccgttac accacgcttg ataaagtggt
    62281 ctttattaaa ctttgttgtt tgaacgccgg gaatctcagg cagtagcgaa gcgaccggaa
    62341 ctttatctcc cgtgagcgcc tcaaaatcct gcataaatga aggtgaatct ggcccgaact
    62401 ggaaagcatg gtctaatgac agcaatgctt cccgtagctt ttcagcttcc agtccgtctt
    62461 cccagatatc atcccaacga cgttgatctt ttggcgcgaa gctggtttgt aatagtccca
    62521 gcaaaaactg ccatgccgcc ccctggagat ctgcccgcgg cgcagcgata tcgacaacat
    62581 tttcatccgc cagatcgact ggcgccagtt tgcctgtcgt tccgtcttta taacgaacgg
    62641 gcaaccacgg ggttgtcaga agtgaaaacg agttcatacg catcctctcg taaaaaagac
    62701 catccctggt cggttgtcca tcattccatc cttttcggtg tatcaactat tcagagagcc
    62761 ctgtcgcagg catctcaacc aaccttgcca gtcaatcctt ccatagccga atacccgcaa
    62821 ctttcgtcat cggtgactaa aatcacgttt gccatctccg gctcttgccg ctgttcaatg
    62881 caccactgcc tgaacgcatc cccttccagt aaagaaaact catcccgatg ttttttccac
    62941 cagcttcgac gcactctgac aacgctcatt tcccatgcgt gagcaccgtt ggcataaggc
    63001 ttcaccacac cggcaataca ggtagacagc cacagggaaa cagactcctc agccagacgt
    63061 gtcgacagtt tttccgggag gtaatcgttg atattggcgg catagccggg cctgaagttc
    63121 agcacaaact ttttagccat tgcgcgatcg cagtaatatt tgcccacttg ttcctgctcg
    63181 ctgcgggcaa atccttccgg cattaccacg tcctcaccgt agactgattc aataagaagg
    63241 cgggctgcgt gtggcatttg aatcgcgcct tgctcacgca gtacacgctg cgtcagccag
    63301 attcgtccat gatcgggata gacatatgca ctgttacgca tggcactgcc gaaccattcg
    63361 tcacctggag cgtcgtccca gacgggggcc agaatcagca attcaggagg ggaacgctcg
    63421 tcttttccgt cacgctttaa ctgaccatta atatcgcgga tatgccgctg taatcgcccc
    63481 gcccgttgga tcagcaaatc aacaggggcc aggtcggata tcatttcgtc caggtcaata
    63541 tccacacttt gttcaattac ttgtgtacaa acaatgactt gcgatgatct ctgtaaacta
    63601 caacttttac cgaagcgtgc cagcgtttcc gtttcaattc gctggcgatc gctaaaagca
    63661 aagcggctat gaaacagaga aaggctggaa gcaggaatga cgccgcgggc aagcaactga
    63721 cgataaacct taatagcgtc atcgacagaa ttccggatcc aggcgatgca tttcccctga
    63781 cttactgccg attcgatacg cgcaatacat tcttgttcgc tatgaagcca acccacgctc
    63841 acgctacgct caacgtcttt gcgcgttgct acccggtgtg aatgcacatc cgatttcgta
    63901 acgtgcgtca gccaggggta atcatccttt tccaggaacg gagcttcttg ctggccctct
    63961 gcaccacgcg caaaggcagc aacgagtttg tcgcgctgct gttgagataa cgtggcagaa
    64021 agcaaaataa cgctgtttcc tccacgcgcc tgccgctcaa tcaatccttc aagaatgcac
    64081 gacatgtaag cgtcacaggc atggatctca tcagccagca ggattttgtt actcagtccc
    64141 agaagccgca gattattatg tttaaacggc atcaccgcca tcatcgcctg atccagcgtg
    64201 ccgacgccaa tttcagccag caacgccttc ttgttactgt cggcaaacca ggccgcacat
    64261 ccctgactga acgcttgttc atccggttct tctgacccga ctaaatcacc ggaccagagt
    64321 gattcattaa agcggtccat taatgtgcgg gcactgtgtg ccagcaccag gctggggcgg
    64381 gattctggcg aatagaaagc aagccaggtt tttaccagcc gatcgtacat ggcattggcc
    64441 gttgccattg ttggcaggcc aaaaaacaaa ccctgtgctt tccccgcagc catcaacctg
    64501 tgggccagga taagcgccgc ttctgtttta cctgcgccag tcacatcctc cagaataaat
    64561 aactgtggcc caggctggct gatatccaga tccagcacct tttgctgtaa cggtgtcggg
    64621 tgctcaataa aaggaaacag cgtattaatt ccggtgaaag gcgcggtttg tgcttttgga
    64681 ggaaagacgg ttaaggcgtt ttgagcctga actaaagcct tctgccagta atctttaata
    64741 tccattgcct gtgcaacgcg tggaaaaaac cgcgttgacg atcccgtcca gtctgcgagt
    64801 acgacggttg cagagatata ccaggaaagt tgttttaaaa gttcaatgcc ctcgtcatca
    64861 tcccagaatg cggggatctc gatgagcgga aacagtacct tgatttcaag gagaaaatct
    64921 cgtgcggcag ctttgtcttc aggcagaaaa ttatccagtt catcaatacg gtcagggggc
    64981 cgaccatgat gcccggtagt catgggcatc cacatctcca tcacccgttt aagtttgcgc
    65041 ggagagagtg aagatgaagg aagcaattct tcgtattcac ttaaataatg atgccacagc
    65101 cagtagccca gcgttgaatg agagatcttt tcgtaattct ttcttgcgcc ttccggaacc
    65161 ttgagttgtg ggtgcaggta aagttgctga aaagagcggg caaattttcc agtatcgtgc
    65221 cagcacaata gccaggcgaa aaattgtgct gcctgctccg tatcagcgaa acctagttga
    65281 cgaaaatagt cagtcagccc gaagcaattg cttttaacca ttaaatagcc catcgcggct
    65341 acatccagcg aatgccagca aagaaggtga tagtcgtcgc ccccgtcttt ctcgccgcgt
    65401 cgggtttttc cccagaaatc aaaaaaagtc acaatatttt tatccttcag taaacttaaa
    65461 ggatatttac
//
LOCUS       DACBVI010000007        65453 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.007, whole genome shotgun sequence.
ACCESSION   DACBVI010000007 DACBVI010000000
VERSION     DACBVI010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.007"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            20..538
                     /locus_tag="IU325_02365"
     CDS             20..538
                     /locus_tag="IU325_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAP5807881.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            554..1093
                     /locus_tag="IU325_02370"
     CDS             554..1093
                     /locus_tag="IU325_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5807882.1"
                     /translation="MKKVFLCAILTSLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1188..2765)
                     /gene="guaA"
                     /locus_tag="IU325_02375"
     CDS             complement(1188..2765)
                     /gene="guaA"
                     /locus_tag="IU325_02375"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP5807883.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     TRIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2834..4300)
                     /gene="guaB"
                     /locus_tag="IU325_02380"
     CDS             complement(2834..4300)
                     /gene="guaB"
                     /locus_tag="IU325_02380"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004159092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP5807884.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4462..5832
                     /gene="xseA"
                     /locus_tag="IU325_02385"
     CDS             4462..5832
                     /gene="xseA"
                     /locus_tag="IU325_02385"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP5807885.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKSLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     AQQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPRIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5829..6044)
                     /locus_tag="IU325_02390"
     CDS             complement(5829..6044)
                     /locus_tag="IU325_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5807886.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6114..7586)
                     /gene="der"
                     /locus_tag="IU325_02395"
     CDS             complement(6114..7586)
                     /gene="der"
                     /locus_tag="IU325_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP5807887.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7704..8882)
                     /gene="bamB"
                     /locus_tag="IU325_02400"
     CDS             complement(7704..8882)
                     /gene="bamB"
                     /locus_tag="IU325_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HAP5807888.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8893..9513)
                     /locus_tag="IU325_02405"
     CDS             complement(8893..9513)
                     /locus_tag="IU325_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP5807889.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9531..10805)
                     /gene="hisS"
                     /locus_tag="IU325_02410"
     CDS             complement(9531..10805)
                     /gene="hisS"
                     /locus_tag="IU325_02410"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP5807890.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNCTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10916..12034)
                     /gene="ispG"
                     /locus_tag="IU325_02415"
                     /gene_synonym="gcpE"
     CDS             complement(10916..12034)
                     /gene="ispG"
                     /locus_tag="IU325_02415"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HAP5807891.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFEQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12061..13074)
                     /gene="rodZ"
                     /locus_tag="IU325_02420"
     CDS             complement(12061..13074)
                     /gene="rodZ"
                     /locus_tag="IU325_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HAP5807892.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13359..14513)
                     /gene="trmG/rlmN"
                     /locus_tag="IU325_02425"
     CDS             complement(13359..14513)
                     /gene="trmG/rlmN"
                     /locus_tag="IU325_02425"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HAP5807893.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14663..15094)
                     /locus_tag="IU325_02430"
     CDS             complement(14663..15094)
                     /locus_tag="IU325_02430"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP5807894.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15243..17555)
                     /gene="pbpC"
                     /locus_tag="IU325_02435"
     CDS             complement(15243..17555)
                     /gene="pbpC"
                     /locus_tag="IU325_02435"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HAP5807895.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADAEGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPTNLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPDSVSRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLMDKGDYQLLVMDEVGQIATVKFVMQ"
     gene            complement(17556..22517)
                     /locus_tag="IU325_02440"
     CDS             complement(17556..22517)
                     /locus_tag="IU325_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HAP5807896.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     VSSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVIEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSEKLLQMADLVYTGLFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIQVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVRFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPTKMRPNQPLTVKIKASNKNGEMPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSVSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGVSRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDVINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQNTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVTNSGDQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGQSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22724..23569
                     /gene="sseA"
                     /locus_tag="IU325_02445"
     CDS             22724..23569
                     /gene="sseA"
                     /locus_tag="IU325_02445"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HAP5807897.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNATFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGAFNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPLK"
     gene            complement(24069..24845)
                     /gene="sseB"
                     /locus_tag="IU325_02450"
     CDS             complement(24069..24845)
                     /gene="sseB"
                     /locus_tag="IU325_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HAP5807898.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEVLEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQAAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(24964..26247)
                     /gene="pepB"
                     /locus_tag="IU325_02455"
     CDS             complement(24964..26247)
                     /gene="pepB"
                     /locus_tag="IU325_02455"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HAP5807899.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEDWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLRDQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     AAQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26492..26692)
                     /gene="iscX"
                     /locus_tag="IU325_02460"
     CDS             complement(26492..26692)
                     /gene="iscX"
                     /locus_tag="IU325_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HAP5807900.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26704..27039)
                     /gene="fdx"
                     /locus_tag="IU325_02465"
     CDS             complement(26704..27039)
                     /gene="fdx"
                     /locus_tag="IU325_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HAP5807901.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDVALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27041..28891)
                     /gene="hscA"
                     /locus_tag="IU325_02470"
     CDS             complement(27041..28891)
                     /gene="hscA"
                     /locus_tag="IU325_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HAP5807902.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSTAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28908..29423)
                     /gene="hscB"
                     /locus_tag="IU325_02475"
     CDS             complement(28908..29423)
                     /gene="hscB"
                     /locus_tag="IU325_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HAP5807903.1"
                     /translation="MDYFTLFGLPACYQLDTQALSLRFQDLQRQYHPDKFSSGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29519..29842)
                     /gene="iscA"
                     /locus_tag="IU325_02480"
     CDS             complement(29519..29842)
                     /gene="iscA"
                     /locus_tag="IU325_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HAP5807904.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29859..30245)
                     /gene="iscU"
                     /locus_tag="IU325_02485"
     CDS             complement(29859..30245)
                     /gene="iscU"
                     /locus_tag="IU325_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HAP5807905.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30273..31487)
                     /locus_tag="IU325_02490"
     CDS             complement(30273..31487)
                     /locus_tag="IU325_02490"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HAP5807906.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31599..32087)
                     /gene="iscR"
                     /locus_tag="IU325_02495"
     CDS             complement(31599..32087)
                     /gene="iscR"
                     /locus_tag="IU325_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HAP5807907.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(32357..33097)
                     /gene="trmJ"
                     /locus_tag="IU325_02500"
     CDS             complement(32357..33097)
                     /gene="trmJ"
                     /locus_tag="IU325_02500"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HAP5807908.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            33216..34019
                     /gene="suhB"
                     /locus_tag="IU325_02505"
     CDS             33216..34019
                     /gene="suhB"
                     /locus_tag="IU325_02505"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HAP5807909.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34164..35018
                     /locus_tag="IU325_02510"
     CDS             34164..35018
                     /locus_tag="IU325_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP5807910.1"
                     /translation="MALPVNKRVLKILFILFVVAFCIYLIPRIAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLHGWFIPSATGPAENAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESCSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35209..36489
                     /gene="csiE"
                     /locus_tag="IU325_02515"
     CDS             35209..36489
                     /gene="csiE"
                     /locus_tag="IU325_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HAP5807911.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALTGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKVFLQTGAPRSAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36481..37620)
                     /locus_tag="IU325_02520"
     CDS             complement(36481..37620)
                     /locus_tag="IU325_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HAP5807912.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVSMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37780..38670)
                     /gene="hcaR"
                     /locus_tag="IU325_02525"
     CDS             complement(37780..38670)
                     /gene="hcaR"
                     /locus_tag="IU325_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HAP5807913.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTEQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTV"
     gene            38806..40167
                     /locus_tag="IU325_02530"
     CDS             38806..40167
                     /locus_tag="IU325_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HAP5807914.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDSIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40164..40682
                     /locus_tag="IU325_02535"
     CDS             40164..40682
                     /locus_tag="IU325_02535"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HAP5807915.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDGNWRLLERDIVLDQ
                     AVISSHNLSVLF"
     gene            40682..41002
                     /locus_tag="IU325_02540"
     CDS             40682..41002
                     /locus_tag="IU325_02540"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HAP5807916.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            40999..41811
                     /locus_tag="IU325_02545"
     CDS             40999..41811
                     /locus_tag="IU325_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HAP5807917.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41821..43023
                     /locus_tag="IU325_02550"
     CDS             41821..43023
                     /locus_tag="IU325_02550"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HAP5807918.1"
                     /translation="MKEKTIIIVGGGQAAAMAAALLRQQGFTGELHLFSDEQHLPYER
                     PPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVTIKTLGRDTQELVLANGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRGCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVHILLNNAIEHVVDGENV
                     ELTLQSGETLRADVVIYGIGISANDQLAREANLDTTNGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAASAMLGLPLPRLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRLIRKWIQSGKTFDAKLLTD
                     EHIALKSL"
     gene            43120..43542
                     /locus_tag="IU325_02555"
     CDS             43120..43542
                     /locus_tag="IU325_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HAP5807919.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(43590..44462)
                     /locus_tag="IU325_02560"
     CDS             complement(43590..44462)
                     /locus_tag="IU325_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HAP5807920.1"
                     /translation="MTIYTLSHGPLELDVSDRGGVIEGFWRDATPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGQWQCVSRSEDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGTETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPHQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVALL"
     gene            complement(44474..45568)
                     /locus_tag="IU325_02565"
     CDS             complement(44474..45568)
                     /locus_tag="IU325_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP5807921.1"
                     /translation="MKINRIVYEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGEGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFTLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(45601..46599)
                     /locus_tag="IU325_02570"
     CDS             complement(45601..46599)
                     /locus_tag="IU325_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP5807922.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(46624..48135)
                     /locus_tag="IU325_02575"
     CDS             complement(46624..48135)
                     /locus_tag="IU325_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP5807923.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVSPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(48158..49141)
                     /locus_tag="IU325_02580"
     CDS             complement(48158..49141)
                     /locus_tag="IU325_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP5807924.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKARVPGAQIVANQEGTVLDKAISVGEKLIIST
                     SDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(49238..52519)
                     /locus_tag="IU325_02585"
     CDS             complement(49238..52519)
                     /locus_tag="IU325_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HAP5807925.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGIVQNASRDAVIKLQRSERGIEWGLYAISPLKGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIILSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLATRNGHFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKITSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKLWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNRVH"
     gene            52637..53830
                     /locus_tag="IU325_02590"
     CDS             52637..53830
                     /locus_tag="IU325_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP5807926.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQGRDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGEHWLNTIIRQTGFNPFDRDEGPSVKATKIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(53894..55147)
                     /locus_tag="IU325_02595"
     CDS             complement(53894..55147)
                     /locus_tag="IU325_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP5807927.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            55475..56665
                     /gene="hmpA"
                     /locus_tag="IU325_02600"
     CDS             55475..56665
                     /gene="hmpA"
                     /locus_tag="IU325_02600"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HAP5807928.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQALPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLGKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(56710..57048)
                     /gene="glnB"
                     /locus_tag="IU325_02605"
     CDS             complement(56710..57048)
                     /gene="glnB"
                     /locus_tag="IU325_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HAP5807929.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(57109..58443)
                     /gene="glrR"
                     /locus_tag="IU325_02610"
     CDS             complement(57109..58443)
                     /gene="glrR"
                     /locus_tag="IU325_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HAP5807930.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(58433..59146)
                     /gene="qseG"
                     /locus_tag="IU325_02615"
     CDS             complement(58433..59146)
                     /gene="qseG"
                     /locus_tag="IU325_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HAP5807931.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAINTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYNDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(59311..60738)
                     /gene="qseE"
                     /locus_tag="IU325_02620"
     CDS             complement(59311..60738)
                     /gene="qseE"
                     /locus_tag="IU325_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HAP5807932.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLVDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADGAVELENVELAPLVEMV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(61314..65201)
                     /gene="purL"
                     /locus_tag="IU325_02625"
                     /gene_synonym="purI"
     CDS             complement(61314..65201)
                     /gene="purL"
                     /locus_tag="IU325_02625"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HAP5807933.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHTIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGLGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGAEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTREVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAVQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFSEELGAVIQVRAADRESVEAVLAQHGLADCVHYVGQAVSGDHFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
BASE COUNT        15469 a        18171 c        16488 g        15325 t
ORIGIN      
        1 atgtgtataa caaggaatag tgatgaaatt taaaaaatgt cttctgcctg tggcaatgtt
       61 agcgtcattc actctggcag gatgccagtc aaatgctgat gatcatgccg ccgatgttta
      121 tcaaaccgat caactgaata ccaaacaaga aactaaaacc gttaatatta tttccattct
      181 tcccgcaaaa gttgccgtag acaactccca aaataaacgg aacgcacaag ccttcggcgc
      241 gcttattggt gcagtcgctg gcggtgtaat cggccacaac gtggggtctg gcagcaattc
      301 cggaacgacg gcaggtgcag ttggcggcgg agctgttggc gcggcagcgg gttctatggt
      361 gaatgataaa accttagtgg aaggtgtttc tctaacgtat aaggaaggca ccaaagtgta
      421 tacctctacc caggtgggta aagagtgcca gtttacgaca ggtttagccg ttgttattac
      481 cacgacgtat aacgaaacgc gtattcagcc aaatacgaaa tgtcctgaaa agagctaatg
      541 atcaggagga gtcatgaaga aagtttttct ttgcgccatc ttaacctcct taagctatcc
      601 ggctatcgcc tcatcattgc aggatcaact ctctgctgtc gcagaagcgg aacagcaagg
      661 taaaaatgaa gagcaaaggc agcatgacga atgggtcgcg gagcgcaaca gggaaatcca
      721 gcaagagaag caacgtcgcg caaacgccca ggccgccgct aacaaaagag cggcaacggc
      781 agcggcaaat aagaaagctc gtcaggataa actggacgcc gaagccactg cggacaaaaa
      841 acgcgatcaa agttatgaag atgagctacg cagcttagag attcagaaac aaaaactggc
      901 gctggcgaaa gaagaagccc gcgttaagcg agaaaacgaa tttatcgatc aggaactgaa
      961 gcacaaagct gcgcaaaccg atgtggtgca atctgaagct gacgcaaaca ggaatatgac
     1021 tgagggcggt cgcgatctga tgaaaagcgt gggcaaagca gaagagaaca aatcggacag
     1081 ctggtttaat taatcgatgt aagtaacttc aagcctataa ttcttgaaga taaaaaaccc
     1141 tctgtagtaa cagagggttt tgttcattca tagtgcaggg atcaaaatca ttcccactca
     1201 atggtagctg gtggcttgcc gctgatgtca tacaccacgc gggaaatacc gttcacttca
     1261 ttgataatgc ggttggaaac gcgaccgagg aaatcgtacg gcagatgcgc ccagtgtgcg
     1321 gtcataaagt cgatggtttc gacagcacgc agagaaacaa cccagtcata cttacgacca
     1381 tcgcccatta cgccaacgga acgtaccggc aggaagacgg tgaatgcctg gctgactttg
     1441 tcgtacaggt ccgctttacg cagttcttca atgaagatgg cgtcagcacg gcgcagcagg
     1501 tcacaatact ctttcttcac ttcacccaga acacgaacgc caaggcctgg tcccgggaac
     1561 gggtgacggt acagcatgtc gtacggcagg cccagctcca gaccaatctt acgcacttcg
     1621 tctttgaaca gctctttcag cggttcaacc aggcccatct tcatctcttt cggcaggccg
     1681 cccacgttgt ggtgagattt gatgacgtgt gctttaccgg ttgcagacgc cgcagattcg
     1741 ataacgtcag ggtagatagt accctgcgcc agccacttca cgtcttccag tttcagcgct
     1801 tcttcatcga atacttcaac gaaaacgcgc ccgatgattt tacgttttgc ttccggatcg
     1861 ttttcgccag ccagcgctga caggaagcga tcttctgccg gaacgtgaac aatattgaga
     1921 ccaaagtgat cgccaaacat atccagaacc tgctctgctt cgttgagacg cagcaggccg
     1981 ttgtcgacga atacgcaagt caggttttta ccgatagcgc ggtgcagcag cattgcggtt
     2041 acggaggaat ccacaccacc agagaggccg aggatgactt tatcgtcgcc tacctgctcg
     2101 cggatgcgag ttacagcatc gtcgataatt ttcgctggcg tccacagggc ttcacactgg
     2161 cagatatcac gcacaaaacg ctccagcatg cgcataccct ggcgggtgtg agtcacttcc
     2221 gggtggaact gtacgccata gaagcgtttt tcttcgttag ccataatggc aaacgggcag
     2281 ctttcggtgc tggctacggt gacgaagtcg gacgggatag cggtaacttt atcgccgtgg
     2341 ctcatccaga catcgagcag aggtttaccg tctgcggtca gagcatcttc aataccgcga
     2401 accagtgcgc tgtcgtttac gacttcaacc tgcgcgtagc caaattcacg ttcgttagag
     2461 gcttcaacgt gaccgcccaa ctgcattgcc atggtctgca tgccatagca aacgccgaat
     2521 accggtacgc ctgcttcaaa gacatactgc ggcgcacgcg ggctgttttc ttcggtggtg
     2581 ctttccgggc cgccggaaag aataatgccg cttggattga agtcacgaat ttgtgcttct
     2641 gtcacatccc acgcccacag ttcgcagtaa acgcccagct cacgcacgcg gcgcgcaacc
     2701 agttgagtat actgagaacc gaagtccaga atgaggatgc gatgcttatg aatgttttcc
     2761 gtcattgacg cttattccga ggcaagtgaa acagataata taaatcgccc gacatgaagt
     2821 cgggcgaaga gaatcaggag cccagacggt agttcgggga ctctttagta atggtcacgt
     2881 cgtgaacgtg gctttcctga atgcccgcac cgctgatacg tacaaactcc gctttagtac
     2941 gcagttcgtc gatagtacca cagccggtca gccccataca ggagcgcagg ccgcccatct
     3001 gctggtgaat gatctctttc aggcgacctt tataggctac gcgaccttcg ataccttccg
     3061 gcaccagttt gtcggcagcg ttgtcgctct ggaagtaacg gtcagaagaa cctttggaca
     3121 tcgcgcccag ggaacccatc ccacggtaag atttgtaaga acggccctgg tagagttcga
     3181 tttcacccgg agattcttca gtacccgcca gcatggaacc caccattacc gcgcttgcgc
     3241 cagcggcgat agctttggcg atatcgcctg agaaacggat accgccgtca gcgataaccg
     3301 gaataccggt gccttccagg gcttctactg catcagcaac ggcggtgatc tgtggaacac
     3361 cgacgccagt cacgatacga gtcgtacaga tagagcctgg gccgataccg actttaaccg
     3421 cgctgcaacc agcttctgcc agagcgcgtg cacctgcagc tgttgccacg ttgccgccga
     3481 tgatttgcag atcaggatat ttagcacggg tttcacggat acgttgcaga acgccttctg
     3541 agtgaccgtg agaggagtcg atcagcagaa cgtcaacgcc tgcggcaacc agcgcgtcaa
     3601 cacgctcttc gttacccgca cccgcgccaa ccgctgcacc aacacgcaga cggccttgct
     3661 cgtctttaca ggcgttcggt ttacgttccg ctttctggaa gtctttcacg gtgatcatgc
     3721 cgatcaggtg gaattcgtca tcaaccacca gcgccttttc aacgcgtttt tcgtgcattt
     3781 ttgccagcac cacttcacgg gcttcgcctt cacgcacggt taccagacgc tctttcggcg
     3841 tcatgtaaac gctaaccggt tgattcagat cggtcacaaa acgcacgtca cgaccggtga
     3901 tgatgccgac cagttcgttt tcttcggtaa ccaccgggta gcccgcaaag ccgttacgct
     3961 cggtcagttc tttcacttca cgcagggtgg ttgtcggcag cacggtctgc ggatcagtta
     4021 ccacgccaga ttcgtgtttt ttcacacggc gaacttcttc tgcctggcgt tcaatggaca
     4081 tgtttttgtg gataaagccg ataccgcctt cctgagccag agcaatagcc aggcgcgctt
     4141 ccgttacggt atccattgct gcggaaagca tagggatatt cagacgaata gttttcgtca
     4201 gctgggtgct gaggtcagca gtattcggca gaacggtaga atgagcagga acgaggagaa
     4261 cgtcgtcaaa cgtcagagct tctttagcga tacgtagcat gggcaatatc tcgaccagag
     4321 tggttaataa atattgccgc ggcattatac agagcgtaac cgattgcatc tacccctttt
     4381 tgcaaaaaat gcttgctatc cccggagggc gggttactat cgactgaata acctgctgat
     4441 ttagaatttg atctcgctca catgttacct tctcaatccc ctgcaatttt taccgttagt
     4501 cgcctgaatc aaacggttcg tctgctgctt gagcatgaga tgggacaggt ttggatcagc
     4561 ggcgaaatct ctaatttcac acaaccggct tccggtcact ggtactttac actcaaagac
     4621 gacaccgccc aggtacgctg cgcgatgttc cgcaacagca accgccgggt gaccttccgc
     4681 ccacagcatg ggcaacaggt tttagttcgc gccaatatta cgctctacga gccgcgcggt
     4741 gactaccaga ttatcgtcga aagtatgcag cctgccggtg aagggttgct gcaacaaaaa
     4801 tacgaacagc ttaaagcgaa attgcaggct gaaggtctgt tcgatcagca atacaaaaaa
     4861 tcactcccct ctcctgcgca ttgcgttggt gtgatcacct caaaaaccgg tgctgcgcta
     4921 catgatattt tgcatgtatt aaaacgtcgc gatccttctc tgccggtgat catctacccc
     4981 accgccgttc agggcgatga cgcgccaggg caaattgttc gcgccattga actggcgaat
     5041 cagcgcaacg agtgcgacgt gttgatcgtt gggcgcggcg gcggttcgct ggaagattta
     5101 tggagtttta acgacgaacg tgtagcgcgg gcgatttttg ccagccgcat tccggtcgtc
     5161 agcgccgtcg ggcatgagac ggatgtgacg attgccgact ttgtcgctga tctgcgtgcg
     5221 ccaacgccgt ctgccgccgc tgaagtagtg agccgcaatc aacaggagtt actgcgtcag
     5281 gtgcagtctg cgcagcagcg cctcgaaatg gcaatggatt attatctcgc caaccgcacg
     5341 cgtcgtttta cgcagatcca tcatcgctta cagcagcagc atccacagct ccggctggca
     5401 cgccagcaaa ccatgcttga gcgcctgcaa aagcggatga gctttgcgct ggaaaatcag
     5461 cttaagcgtg ccgggcaaca gcagcagcgg ttaacacagc ggctaaacca gcaaaatccg
     5521 cagccgcgta ttcaccgcgc acagacgcga attcaacaac tggaatatcg cctggcagaa
     5581 acgttgcgcg cgcaactgag cgcaacgcga gaacggtttg gtaatgcagt aacgcacctg
     5641 gaagcagtta gcccgctttc gacgctggcg cgtggataca gcgttactac tgctactgac
     5701 ggcaatgtac tgaaaaaagt gaagcaagtt aaagcgggtg aaatgctaac cacacgtctg
     5761 gaagacggct ggatagaaag tgaagtaaaa aacatccagc cggtaaaaaa atcgcgtaaa
     5821 aaggttcatt aagccagcac gaattcaacg cgttttttcg atatcaagcc atgcccgtgc
     5881 tgacagaagt aatctactgc accgcaggct tttaacacct gcaacggttg atggcagtct
     5941 ggacaaagag ctttcatttc aataaattcc ccgcaggatg ggcaacgcgc atggccgtta
     6001 tcctgatcaa gcacatgctg acattgtgga caatgcagtt ccatatgaat ttctccggta
     6061 gccattccct ctacattcat agagggaatg gcagataaaa tacttacgga taattattta
     6121 tttttcttga tgtgcttcat cagacgctta cgtttacgca tctgggttgg cgtcagggtg
     6181 ttacgcttgt tcgcatacgg gttttcccct tctttgaact gaatacgaat cggcgatccc
     6241 attacgtcca gcgatttgcg gaagtagttc atcaagtaac gcttgtagga atcaggcagg
     6301 tctttcacct gattaccgtg aatcaccaca atcggcgggt tataaccacc ggcgtgggca
     6361 tatttcagct tcacacgacg accgcgtacc aacggcggtt ggtgatcttc aacagccatc
     6421 gtcatgatgc gcgtcagcat agaggtcccc acacgacggg tggagctgtc atacgcttca
     6481 cgtactgatt caaacagatt accaacacca ctgccgtgca aggctgagat aaaatgcaca
     6541 cgagcaaaat cgataaagcc cagacggaag tccagcgttt ctttcacctg ctctttcact
     6601 tcctggctca ggccatccca cttattcacc acaatgacaa gtgagcgccc actattgaga
     6661 ataaagccca gcagggagag atcctgatcg gaaatacctt cgcgcgcatc aatcactaac
     6721 atcaccacgt tggcatcttc aatggcctgc aacgttttga ttaccgagaa tttctctaca
     6781 gcatcggtga ttttgccgcg tttacgtacg ccagcggtgt caatgagcac atactcacgt
     6841 ccatcgcgtt ccattggaat gtagatactg tcacgcgtcg taccaggcat atcataaaca
     6901 acaacgcgct cttcaccaag aatacggtta gtgagtgtag acttacctac gttcggacga
     6961 cccacaatcg ccagtttgat cggcagactt tgcgggtcga agtcgtcttc ctcttcttct
     7021 tcgccgttct cttccgcttc aaattgcgcc cagtattcgg cgtcttcgtc gacttcttct
     7081 tgcggtgcga gatcttccat ccacggcagc agcacgtgct ccagcagact taatacgcca
     7141 cgaccgtgag acgcggcgat cgggtagatt tcccctaaac caagcgagta gaaatcaacc
     7201 accgcctgat cgggatccag accgtcagtt ttgtttgcca ccaggaaggt cggtttttca
     7261 cgggagcgca gatgtttggc aatcgcttca tccgccggca tcaggcccgc gcgcgcatcc
     7321 accataaaca gtacgacgtc cgcttcttca atcgccagca gcgactgttc cgccatacgt
     7381 gtctctacac cgtcttcggt gccatcaatc ccaccagtat caatacaaat aaactcgcgg
     7441 ccttcaattt ctgcacgacc gtacttacgg tcacgagtca gacccgggaa atccgcaacc
     7501 agcgcatctc gggtgcgagt tagacggtta aataacgtgg attttcctac gttagggcgc
     7561 ccgacaagcg cgaccacagg taccatgttt aaagcctcat ttttataaat catcagacaa
     7621 cgcacgctat attcgtgtcg ttgttaaaaa caggaaaacg gcccctgtcc aggagccgtt
     7681 ttcaaagtga acgacagaga cgattaacgt gtaatagagt acacggttcc gtcttttgcc
     7741 tggatcagca gtttgccatc agcggcaacc ggttcagtct ggaaaccgga actatcaact
     7801 ttttgctggg caacgaaacg accatcttcg acgttaatcc agtgcagata accttcgctg
     7861 tcacctacca ccaggttgcc attatacagc accggagaag tcagcaggcg atgcaacaga
     7921 tcgctttgtg tccacagcgt aacgccgcca tcaatggtca acgccatcac ccggtcattt
     7981 tgatcgacca gatagatgcg attgccgtcg acgatgaaat cattcaccga acccagttcg
     8041 cgtttccaca taatctgacc actgcgcaga tcaagcgccg tcaggttacc attataggcc
     8101 agcgcgaaaa caacgccgtt aacaacgacg ggagtcgtgt caacatcgct cagacggtca
     8161 atttcggtag aaccggtcgc ctgggaaata cgctgctgcc aaatcatctg gccctgttcc
     8221 atcagcactg cgctgacgcg accattatcg ccacccacga cggccgcacc aaaagccgtt
     8281 gccggcgcag actcgccacg caaagagagc gaaggcatat cgaggttaac tgtccatttg
     8341 acagcgccgt cagcttcgtt cagcgcttgt aactgaccgt tactggtgtg gattaacacc
     8401 agaccgtcgc tgaccaccgg acgcgaaagt gcttcacccg cgactttagt ttgccatgcc
     8461 acagtaccat cgctggtatt cagcgcgtaa acctgcgcct tttcgctgcc aatgtagaca
     8521 tgcccaccag acacggtcac accgccagaa agtaatgcag gctctttaga gaaccagcca
     8581 tctttctcgg ccaggctgac agaccagatt tctttgccat catccgcatt cagcgctttt
     8641 actaaaccag cgcggtccgc tgcatagaca acgttgtccg ccagtgccgg atgaagattg
     8701 gaatagaagt tgccaatgcc gctaccaacg gaagtgctcc acgccgtggt cggcgtaaac
     8761 tggttttcaa cggtcggcaa tggggacatc tttaccacat cttcttcgct gttaaacagc
     8821 gaacagccgc ttaaaagggt aacggaaagc agtcctggca gcagtaattt acgcaattgc
     8881 atcgggtccc tctcagatgg acaaattatt aattttcatc tgcatcattt cgctcagtgc
     8941 cggggtaaca tcgcttttca cgcctgcttc ccatgcacta cgcgcacctt gcttatcacc
     9001 tttgctcagc aatgcttcac cacgcaggtc ggcaacaatg gcagcccacc cttcaccttt
     9061 gatggtatca agggttttca gcgcggcatc agcctgcttg agctgaacct gaacgcgagc
     9121 aagacgcaga tttatcacgg ctttgagatt ttcatcgctc gtgtctgcca gcccctgttg
     9181 taactgggcg gcagctttct ctagttcatt tttgtcaaca aattgctgcg caagttccaa
     9241 agaagccagc gcaccataag tatttttatt ttcagcagca aatttttccg ccgccggaat
     9301 gctatccggt ttgccttcgc tcactgcggt aaccgcattt tgataggcaa gagaagcgga
     9361 gcgtgcagaa tcaacctgat ggctgttcca gtagcgccag ccaattagtg cgccaacgcc
     9421 caaaatcacc ccaacagcca gtgctttgcc attttcagca aaaaagcgtt taaccgcttc
     9481 tacctggtcg ttttcgttct cgtaaatttc cacgctgtcc ttctccttcc ttaacccagt
     9541 aacgtgcgca aatgcgcggc tacgctatcc tgcgcaactg ccgtttgctc accagagcgc
     9601 aaatccttca ctactgctgt gccgttagcc acttcagact cacccagcac cacagcaacg
     9661 cgggcacccc atttatcagc acgggcaaac tgtttcttaa agttgccgcc gccgtggttg
     9721 gtcatcaatt tcacgcccgg taattcatca cgcagacgct ctgctaatgc catagccgca
     9781 gattgtgtat cagcacctga agccaccagg tatatatcga caacaggatc ggccttaaat
     9841 tccggattaa cggcctgaac taacaataca agacgttcga ggcccatcgc aaaaccgaca
     9901 gccggtgtag cacgaccacc cagttgttcc accagaccgt cataacgacc gcctgcacac
     9961 acggtgccct gggaaccgag gctgttagtc acccactcga aaacggtaca gttatagtaa
    10021 tccaggccac gcaccagacg ctggttgacg gtgtaagcga tccccgcgct ctcaagcagt
    10081 ttgcacagac cggcaaaatg ctcacgagat tcctcgtcca gataatcgcc taatgccgga
    10141 gcgtcgttga gaagcgcctg cacttccgga ttttttgaat ccagcacgcg cagcgggtta
    10201 gtgtacatgc ggcgtttgca gtcttcgtcc agcttttctt tatgctgctc aaggaatgcc
    10261 accagcgcat cgcggtaatt cgcacgagct tccagcgaac cgatagagtt cagctcaagt
    10321 gttacgtgct cggaaatacc cagcgcgcgc caccagcggg cagtgagcat aatcagttca
    10381 gcgtcgatat ccggaccttg cagaccgaaa acttcgcagc ccaactgatg gaactgacga
    10441 taacgccctt tctgcggacg ctcgtgacgg aacatcggcc cgatatacca cagacgctgt
    10501 tcctgattgt acagaagacc atgctcgatg ccggcgcgta cacagcccgc cgtcccttca
    10561 gggcgcagag tcaggctgtc gccattgcga tcctcaaagg tgtacatctc tttttcaacc
    10621 acgtcggtga cttcaccaat cgcacgtttg aatagcgggg tctgctctac aatcggcaag
    10681 cggatttcac tgtaaccgta gctgccgagc acgtttttca gtgtgccttc aatgcgctgc
    10741 cagatggccg tttcgccagg caggtaatcg ttcatgccgc gaatggcttg aatgtttttt
    10801 gccacgttta ttctctttct gaatataaaa atgaaccctc aacgcttccc tcaatgtttc
    10861 gggagccatg cgggttcaat catacacggg aagcgaggcg cttcccatca cgttattatt
    10921 tttcaacctg ctgaacgtca attcgacgcg cttcgtccag ctgactggct ttcgcacgaa
    10981 tgcgcgcttc cagctggtcg atcatatcgt tgttgtccag acggtctttg cgcacgccat
    11041 cttcatagag gccgcttttc ttgttaccgc cggtgacgcc gagtgtagaa accagcgcct
    11101 cacctgggcc attcaccaca cagccgataa tcgaaacgtc catcggagtg atgatatctt
    11161 ccaggcgttg ctccagcgcg ttaaccgtac cgataacatc aaactcctga cgcgaacagg
    11221 ttggacaggc gatgaagttg atcccgcgcg aacggatacg cagcgatttc aaaatatcga
    11281 aaccgacttt gatctcttca accggatcgg ccgccagcga tacgcgcagc gtgtcgccaa
    11341 tgccttcaga cagcagcaga cctaaaccaa tggcggattt aaccgccccg ctgcgcgcac
    11401 cacccgcttc ggtgatcccc agatgcagcg gctgatcgat ctgttttgcc agcaaacgat
    11461 aagactcaac agcgaggaag acgtctgacg ctttcacgct gactttgaac tgttcgaagt
    11521 tcaagcgatc gagatgatca acatgacgca tggcggattc cagcaacgcc tgcggcgtcg
    11581 gttcgccata cttttcttgc agatcttttt ccagcgatcc ggcgttaacg ccaatacgga
    11641 tcggaatgtt tttatcgcgc gcacagtcaa ccaccatgcg aatacgctct tcattaccga
    11701 tattgccagg gttaatacgc agacaatcga cgccgtattc cgctactttc agcgcaatgc
    11761 gatagtcgaa gtggatgtca gccaccagcg gcacgttaac ctgctgtttg atgagtttga
    11821 acgcttctgc cgcgtccatc gtcggtactg agacacggac gatatcagcg ccaacacgtt
    11881 ccaacgcctt gatttgattg accgttgctt cgacgtctgt cgtacgcgta ttggtcatgg
    11941 actgtacggc gatgggagca ccatcgccaa tcggcacatt cccaacgtaa atacgtgttg
    12001 attttctacg ttgaattgga gcctggttat gcatgaaaaa tctcccgcgt tacccgtctg
    12061 ttactgcgcc ggtgattgtt cggcattgag ggtcagacgc gcaacctggt tagttctgat
    12121 aaaacgactc agatcgacag gtttcccttg atactggatc tgtactgcgg ctggcgcacc
    12181 aattttcagt ttatacggtg cctggccggt taaattcaaa ttaccgtctt tacgctgcat
    12241 cccgctaaac agttttttac cggtagcatc agtgacctcc agccagcaat cggcagtaaa
    12301 gttcatcacc agcgcattcg gatcagccgc cggcgtggtc acgccagcct gatcggttgg
    12361 caacggcgca gcaccatctg gcgttgttgt tgccgttggt gccggggtcg cggcggtatc
    12421 aacatttgcc tgcgaaggcg aaacaaccgc attctgttgc ggatcaacag ctggtgctgg
    12481 cgcagttacg gcaggtgttt gcgtgttggt tgcggtagta tccacagacg ctggcggcgt
    12541 ggacgttgta gccgggtctg tagttgtcga cgtatttaac ggaacactct gcccctgctc
    12601 gctattactg ctcaattccg ccgaagattg atcggccata gtggtgatct cttcctgctg
    12661 agctttgtgg tcttgccacc accaggcacc gctcaggccg ataaccacaa acaacaccag
    12721 ccaggtgaag gtcatcagcc agccgtcgcg ttttttgcgg cgtttaccga gggaaaaact
    12781 ctgcatcggc gcaacttttg cagcccgaag tggagcctgc ttttccagcc ctggcagcag
    12841 ttcttcttct ggaatatgta ccagacgcgc ataagagcgg atatatccgc gcaggaatgt
    12901 tgaagcaaga tcggcgggtg ccttatcttc ttcaatgtcg cgtaccgtgg aaaccttcag
    12961 gcaaagtcgc tcggcaacgg cctgctgact aagtcctagt tgttcgcgag cattacgcag
    13021 gcgagcgccg gtagtaagtg cttcattttg gtcgtgcgtg gcttcagtat tcattcgctg
    13081 caggtacgtt taaatgagaa ttgagatgcc ggtgaaccat catgctcacc cacaccgcga
    13141 aacatccgtt aagttaaccg ttatcataca gtataagacc gtctgtgtgc tcgtgacaaa
    13201 gcccacattc actaaggcta acttactgtt gcatcgttac atactgcctt aaagtcagca
    13261 aaaacgcacc gttaatattg accagacaat tgcaacttaa tacttcattc ataaaagatt
    13321 acgccacggt acataaagta accgtggcgt aatggctatc agaccgcttt aatatcgatg
    13381 gcttcaccct gcatccgttt acgcagggta cgtttcgtac ggtcgataac atcgcccgcc
    13441 aactgaccac aggcagcatc gatatcatca ccacgagttt tacgcacaat agtggtgaaa
    13501 ccgtagctca tcagcacttt tgagaaacgg tcgatacggc tgttcgaact gcgtccatac
    13561 ggcgcgccag ggaacgggtt ccacgggatc aggttgatct tacacggcgt atctttcagt
    13621 agctccgcca gttggtgcgc gtgttcagtg ccgtcgttaa cgtggtcaag catcacgtat
    13681 tcaatagtga ctcggccctg attggcgttg gatttctcca gataacggcg taccgcagca
    13741 aggaacgttt cgatattgta ctttttgttg atcggcacaa tttcgtcacg aatttcgtcg
    13801 ttcggcgcgt gcagggaaat tgccagtgca acgtcgatca tatcgcccag tttatccagc
    13861 gccggaacta caccggaagt ggaaagcgtc acgcgacgtt tagacaggcc aaaaccgaaa
    13921 tcatcaagca tgatttccat cgccggaacg acattgttca ggttgagcag cggctcgccc
    13981 atgcccatca tcactacgtt agtgattgga cgctgaccgg tgacttttgc tgcgccgacg
    14041 attttcgctg cacgccacac ctggccgata atttccgata cccgcaggtt gcggttaaag
    14101 ccctgctggg cggtggaaca gaatttacac tccagcgcac accccacctg cgaagagacg
    14161 cagagcgtgg cacggtcgtc ttccgggata tacaccgttt cgacgcgctg atcgccaacg
    14221 gcaattgccc atttaatggt gccgtcagat gaacgctgtt cttcaaccac ttccggtgcg
    14281 cggatctccg ccacctcttt cagtttgccg cgcaacactt tgttgatgtc ggtcatctca
    14341 tcaaagttgt cgcagcaata gtgatacatc cacttcatca cctgatcggc gcggaagggt
    14401 ttttcaccta aatctttaaa aaactcccgc atctgctgac ggttgagatc cagcaggttg
    14461 atttttccat ctttcgtggt gacgttttca ggtgtgacta attgttcaga catatgctat
    14521 tccggcctcg ttattacacg ttatggcccc tggagggttg aaaaaagaaa cgccccggtg
    14581 agcggctgct cgtccggggg cgctgcattg tacaaattct ggcgcacgga tgccacgttt
    14641 gcacgcggca tttacgaaat tattaacggg tgcgcgggca cacttcgcct tcgccgaaga
    14701 aataagcgat ttcgcgagcg gcagattcga cggaatcaga accgtgggta ccattttcgg
    14761 tcaggctgtc agcgtaatca gcacgcagag taccggccag tgcgtttgcc ggattggtcg
    14821 cgcccagcag atcgcggtga cgctgaacgg cgttttcacc ctccagcacg gaaaccacga
    14881 tcgggccaga ggtcatgaat tcaaccagac catcaaagaa cggttttcca tcgtgttcag
    14941 cataaaagcc acgagcctgt tcaacggtca ggtgcagcat tttggtgccg acaattttga
    15001 accctgcagc ttcaaagcgc gcaaagatat tacctatgac gttttttgct accgcgttcg
    15061 gtttgatgat ggaaaaagta cgttcaatag ccatttttac ctctgtaaat tgttctgttg
    15121 ttgtctgtac cagcgtacga gatggcgcgg attataatga gcaacagggc cgttgactat
    15181 tgatgaaggt aacatttttt taaaataaac ctagtttaag caacaatcag ggacaaaaca
    15241 gactattgca tgacaaattt cactgtcgcg atttgcccca cctcatccat caccagcaat
    15301 tgataatcgc ccttatccat caaatgcagg gtcacgttgc gcccgcgttc agttaacggt
    15361 tcgccattca gaaaccacca gcgttcacct gcccctccac ttgattgcaa cggcaaagtc
    15421 gcttccgctg cgcccggtaa acgtttaata atcgcgccat cgcgaacacc tgtcagctgc
    15481 agtgggagtt gtgcatcgtg accgtatggc gggcaaattg tcgaggccgg tggtaagcgc
    15541 acagcgcggc gttctgatgc gggcaaccag ggttccagcg gtagcggcca gacatttatc
    15601 atttcttgtc gcgcctgagg gcaatcagcg gcaacacgtt tgccgttttc atccagccag
    15661 atggggaaac gaatgccatt aatgccttcc tgctccggca gtaatagagt tggtggctga
    15721 ctcccgtcca gcagccaggt tgccaggcgg cggcggcagt taccgtcacc ttccggcaaa
    15781 gactgtccgc ccggccagca gataacgcca cgacttacag agtcaggacg tgggtcttcc
    15841 ggcagattcg cactgcgcga cagtaagata ttattaacct gattcaacaa tggtacggcg
    15901 ctggcaaagc caaactgacc aacaacgggc gtgccgtccg gtctgccagt ccagatccca
    15961 atgacatagc gagcgttaac cccaatcgcc caggcatcac gatagccata gctggtgccc
    16021 gttttccatg ccagtggggc gacgcgcggc aaggcaccat ccggcaaggg ttgcgcttca
    16081 tcagccataa tccggcgaat gatccacgca gcccccgacg acattaaagg ccgttcaagc
    16141 agcggatcgt caggctgtaa gcgcaattta cctgccttgc cgtggcgagc aaacgcggta
    16201 tacgctgccg ccatatcttc cagttttgca ccagcgccgc cgagaatgag cgaaagattc
    16261 ggcgcagcgc cgttgggcag atacaacggc aatccaacat tgcgtaattt tgccgcaaac
    16321 cgtttcggtc cataggcttc cagcacctgc acagcaggta aattcagcga gcgcaccagc
    16381 gcctcgctca tgctgatcgg gccatgaaaa ccgctatcaa agttacctgg tcgataatca
    16441 ccggtgcgcc ggggaacgtc ttgcagcagt gatgccgggt ggatcaagcc ttcatccagc
    16501 gccagaccat aaacaaacgg tttgagcacc gatcctggcg atcggatcgc attgaccata
    16561 tcaacatgac caaagcgtga atcatcgttg agatcaacag aacccaccca gccgcgaaca
    16621 cgcatatcgg tatgatcaac cacgatcatc gccagtgagc tgcgcggtgg taatcgtcct
    16681 ttccagtttt gcgccagttc ttccagacgt cgttgaagac cggcatccag cgtagtaacg
    16741 attttgtcgc ttttgctttt accgagcatc atgcgcgaaa acagcggtgc cagttgcggc
    16801 atttgtcggg gtgccagcca gatgggttct tcccttgatt cttttacctg ctcacgggac
    16861 cacacacctt gcgcagccat ccgttcgagc actttattac gcgcggcttc ggcacgttcc
    16921 ggccagcgat ccgggcgaag acggctggga gcttgcggca aaaccgccag cattgccgcc
    16981 tcggaatagc ttaaattcgt aggcgatttt ccgagataag cccaacttgc cgcaccgatc
    17041 ccctgcaacg taccgccaaa cggggcacgg ttaagataca aggtcagaat ttcacgctta
    17101 gacagatgcc actccagttg caacgcgcgc cagagctggc gaattttgcc gccaaatgtt
    17161 ttggggtgag gatcaagcag acgagcaacc tgcatagtga gcgtgctgcc accggaaata
    17221 acccgtcccg aagtgagatc ttgccatgct gcgcgcgcca ccgagaatgg attcaccccc
    17281 ggatgcttcc agaaccagcg atcttcataa ttgatcagcg cttcaaggta acgtggagaa
    17341 acatcttcga ttgttaccgg ataacgccag atgccttccg catcggcaaa gcgccagagc
    17401 ggcgtcccat cctgcgccac aaccactcgt gcgggattga cttcatgcag cggtagaggc
    17461 cagagtttat ctgctcccca cgctgcaaga aaaaggagaa agggcgcagc tgccagcgtt
    17521 atccagcagc cgcgtttggt taacaggcga ggcatttacg gtctgacaat cagcagatct
    17581 tcagccgcgc cagtcgcccg ccattgggga acatacattg attccaccat cggttgcggt
    17641 acctgatacg ttcccggcgt caccgcccgc gccagataca ccagcgttac cggttggtat
    17701 tcatcaacgg caaccgccgc cacaaagcga tcatcacgga actcaatgtg cttaatgctc
    17761 gcctgctgca tctggttcag taagttctgc acttcgccac cactttgctc caggctggcg
    17821 ctaccgttcg ccagattctg gttttccagt tccagacccg caggcagcag atccacgact
    17881 aacgcatccg gcacgctgtt actggctttt acctgcaacc agaccagcac cagatcgccg
    17941 ctacgtaacg agtccagcga tttactctga ccatcagtac caagaatatg acgctcgatt
    18001 tgcagcacat tgttcgccgg taaaggtgcg gattgcggat aaccgctggc atccacacgc
    18061 agccataacg gctgatcgcc actgttggtc acctgcaagg tggcaagttg atcgctattc
    18121 agattgctgt ttagcgtttt ctcgcctgtc agcggctcag ctgagaaaga ggtttgcgcc
    18181 tgccatttac ccggtaaatc ctgaattgta cgggcagcca ggaacaacgc gttactttcc
    18241 tgggtcgata gccagcgttc accaaatgcc tgctgcgaaa gcgtgttcag caaagtgttc
    18301 tgctcatcgg gtagcagttt attttcttcc agcaaggaga gcatcaacgc gttgtcgcgc
    18361 agtggactac cgtaatcacc cagccatatc cgctcatcac tattacgcgg cgttttcagc
    18421 gccaacgcaa ttgcttcttc accacgcgtc gcatcaccca tggttttcag cgcaacgcca
    18481 agttgcagca gcggtaaacc agaagcggca tctgcacgat gctcccagat ttcacgcagc
    18541 gcacccagtg gtgctttttg ctgacgggcc agcaccagcg cagcgtaaga ctgtacggcg
    18601 aatttactgg ctttgagatt atccgcgtac gggatcgaca tcatgcccgg atcttgtaaa
    18661 tagcgcagca gacgctcatt tccccggtta atgacgtctg tcggcacgct gtacccctgc
    18721 tcacctgcgc ggaccaggaa atccatcacg taagccgtca gccagtactc ttcgtcaccg
    18781 tttttatccc acagcgcaaa gccgccgtta tcacgttgca tttgcagcaa acgggaaacg
    18841 ccgatatcga ccgatgcacg gcgtttctca tcactgtcgc ctttgatgcc caacgcctgc
    18901 agttgggctg cgttggtata aagtgacggg aacaagccgc tggcggtttg ctcaagacag
    18961 ccgtacggat acgcttttaa ctctttgata taacgtgcga tgttcagcgg tggtttgccg
    19021 ctcaacaaca attgcccttc cagtgtaaca ggcgagaagt tttgcaatcc atccgccgga
    19081 atcgcccatg tctcaccagg ctgcaacgcc gtaccgtaat taaccgtttg tgccgggaac
    19141 gccggacgga cgccgatttt ccactgctta tgctgatcgg caacggtttc acccggtaac
    19201 gctaacccgc taatggtggc ctgaatttct ccatcgccat aacccggcaa tgctcgcacc
    19261 gggataaaca gcgtagtacg cacacctggc gctaattcaa cggctgcggg tgaaacgctg
    19321 accagttcaa gcaaaccact ggcggtcagg gcaacgttca gtttttgcgg tttatcggta
    19381 agattagtga tatccagcgt cagacgcgag gtatcgccac tcgccataaa gcgcggcatg
    19441 ttcagttcag caatcaccgg tgcggcaact atcactttgc tttcgttgct accgaagtca
    19501 tctgccgtcc aggcttgcgc catgacgcgt agttcaccgt taaaatcgcc aatcggcagt
    19561 gtaaccgagc cttcgccctg ttcgttgagc gttaccggca gcgcctgctg cgcgacaata
    19621 ttgacgtgat tgaccggcgg tttaccacca cgtttcagct catcaccatc gccaccaaaa
    19681 cgcagagctg ccagacgccc ctgaccttca atgacctgac cgtaaatatc gtaaatgtct
    19741 gcaccatagc gtttctgacc aaagaacgcc tgccacggat cgggggtaac gtaatcagta
    19801 atattcagca caccgctatc aacggcagac accagcacat tcacctgttt tggcatctca
    19861 ccatttttat tgctggcttt aattttcacg gttaatggct gattggggcg cattttggtg
    19921 ggggtttcca gcgccagatc gagacgacgg ttttcatcgc caagcggcag atgcaacacc
    19981 ccaactgcgc gttttggcgt cgcagagcga gatttatcgc caggacgtac caccagcgta
    20041 cttaaataca gatcatgacg attccaggtt ttatcgaccg gaatcgtcag atccagccct
    20101 tgagccggaa catcaatctc ttgccaccac agcggccctt cactggactc gaccatcgca
    20161 taacctttac ccgccgttgg cgcggcgata tgcaacttaa tggtgtcgcc agggcgataa
    20221 ctggctttat ccagtttcag cgtgacacgg tccggacgca ctgcgccgct cccgtcgctg
    20281 ttgtcctgcc agctatagcc agcccagaaa cgcacactac tgaccgcttc attcggcgct
    20341 ttgacttcca gacgataagc gccccactct accgggaaac tgaccttgcc ggtttcgtcc
    20401 gctttcagat ccagagtttg ttcattttca atcagatctt tttgatcaaa ctgtgattgc
    20461 cagccttcat cttctgacca gttccagtag taatcgcggc gttcgcgaat caggcgcacc
    20521 tgcaagcccg acacggcttt tttcacgcct tgcgcatcgc tataaacgat gtcaaaagcg
    20581 gcgttactgc cttcatcaac aatgggctgt ttcaccgtgc tgtcagtgcg ataatcgtaa
    20641 accgatttcg aggcgaactg cggacggatc cccggcaatg catcggcagg ccagatagcc
    20701 tgctcagcgc ggcgcgtcac cgggcgaccg cccgattcca acaaactacc ctggaaaata
    20761 acctgtaatg gggaatgcgt ttccttccac tggctttctg tagaaacttc gccacgccct
    20821 ttatcatcca gcgtcaactg aacttcatcc agcgtgcggg aaagattttc ggcagctata
    20881 tcgccgaatt cgaaaccagg taaggctgac acagcttcac gcagtgggcg caggaaaagt
    20941 tgcccttgca aagtattacc attagcaggc gcaccataca ggtagtaccc caccacggaa
    21001 aatctcactt catctttcgg cgttagcggg gttttctcac cagtcagatt cagcgccatg
    21061 cgctctggca taaaatcttc gacgtggaaa tcccacatcc ggtactggtt atcgcccgtg
    21121 ttagcgcgaa tatgccacat accggttgcc gcattgctat cgagtggcca ggtaaagtgg
    21181 tagaggccat tctccggctg actaacgacg ctcctgagta cctgcccgtc gggtttaatc
    21241 acgtctaact tgatgggttg atcgggcaat gcttttccat ctgcatcacg caataaacca
    21301 ttgaggatga ctgtttcacc agggcgataa agatcgcgcg ggccaaacat gaaaaactgt
    21361 ttgctatagc ctggcgcgcc agcaatgtta aattctgcta agtccagcgc cggaagtttt
    21421 aaatcgagta gcgttgtctg accgtcttta cgcgccagca gtaatgccgc gtttttatca
    21481 ttttccagct gcacatgccc ctgagcatca ctggttgcct gagtcagagt ctgccctttc
    21541 tcatttaata aagagacttg tattccttgc tgggccgcgc cgttttccag actttgggta
    21601 aagatatcca gacgattgtg ataacggtga gctgaaacgc cgatatcact taacgtaaac
    21661 agcgtcgcgg gattactgta atcgtaacgt ccagcctgat tcatcacagc cagatacacg
    21721 cctgcctgct gaagcggttt gatatcaccc agcggcagca ataatttttc acgggtgtta
    21781 cgcgcaggat tgagatcaaa cagtccggta taaaccagat ccgccatctg cagcagtttt
    21841 tcagactgcc agttcgccag cgaattgcgg tattcccatt ggctaatgaa tgcaggcaga
    21901 gattcgggct tcacacggaa gaagttaaca tcaacattgt taacgttgag cgccattacc
    21961 ggcagccctt caataacttt accaggtaac aacgaaccac ggctggcaaa accgacgctg
    22021 ggttggatat cgcgggtagt tatagttttt tcgtaatctt tactgaaggt tgcgttgttg
    22081 agtgctttga cctccttgcc aatagtgacg atcaaatcac gtttcggttc gaggtggcgt
    22141 aaacgcaact ctttaagatt atctgacagc tcccaggcac catccacttt gccgcttttc
    22201 ttatcgacga catggataac gcgtgagaaa tcctgatccg ggtcgagagg aatggagaac
    22261 gtcagcacca gcgtggctgc gccatcaagt tgaacttcag agagatccag caatgtcagc
    22321 gccttaccgg cactctgttg ggccagtttt tgtcgctccg gcgcggaaag ttttgctgaa
    22381 ctcacgtttt ctgaagaggc cgctttagta acttcagaag gcgcatcttt tttcaccgct
    22441 gttggcgcgt tatcgttgtt gtcgcaccct gccagcgcca gcattagcat gcaggcggct
    22501 acgcgtaact ttttcatttt tcatccctgc cacaatggcc cgttagcaac gtcgaataat
    22561 tattatgcgt gagaatttac gttatgtaat tcagtatcac gcccatacaa tccgaatttt
    22621 agtgagaatt gtggctcaga cagccaaatc agcctctttt gcttgttgca tttcactaaa
    22681 catccgacaa tttagcctaa ccccggcaaa aatggagatg cctatgtcca cgacatggtt
    22741 tgtaggagcc gactggctcg ccgaacatat tgatgacccg gaaattcaga ttatcgatgc
    22801 ccgcatggcg tcgcctggac aggaagatcg taacgttgct caggagtatc tgaatggaca
    22861 tattcccggc gcagtgtttt ttgatatcga agcgctttct gatcacactt ccccgcttcc
    22921 gcacatgctg ccgcgcccgg aaacgttcgc cgtggcgatg cgtgagttag gcgttaacca
    22981 ggataagcac ctgattgtct atgacgaagg taatcttttc tcagccccac gagcatggtg
    23041 gatgctgcgc acctttggtg tagagaaagt gtcgattctg gggggtggac ttgcaggctg
    23101 gcagcgcgat gatctgctgt tagaagaagg tgcagtagag ctgccggaag gagagtttaa
    23161 cgccacgttt aatcctgaag ccgtggtgaa agtcaccgat gtgttattgg caagccatga
    23221 aaatacggcg caaattattg atgcccgccc ggctgcacgt tttaacgcag aagttgatga
    23281 acctcgccca ggtttacgtc gcggacatat tcccggcgcg tttaacgttc cgtggacaga
    23341 actggtacgt gaaggcgaac taaaaacgac cgatgaactg gatgcgatat tttttggtcg
    23401 cggcgtaagt tatgacaaac ctattatcgt cagttgcggt tctggtgtaa cggcagccgt
    23461 ggttttgttg gcactcgcga cgctggatgt gccaaacgtg aaactgtacg acggcgcatg
    23521 gagtgaatgg ggcgcacggg cagatttacc ggttgagcca ttgaaataag ctttttacag
    23581 gcaataaaaa accgccgaat ttggcggttt tttattgctg gtctggttcg cggcctttcc
    23641 agctggttga cttgtgttgc tggagcaacg caggcacccc acagtgttga aatactctct
    23701 tttatgcctg gctatgtcaa gataataaac agagtaatta ttcaaacatg agagtattat
    23761 tcgtgacggc ccttttcgcc gtctcgcaaa cgggcgctgg ctttaggaaa ggatgttcca
    23821 tgaccgtaaa tgcagacacc ccacagtgct tgctatcgcg gcaatatcgc cagtggtgct
    23881 gtcgtgatgc ggtcttcgca tggaccgcac aatgaagata cggtgctttt gtatcgtact
    23941 tattgtttct ggtgcgctgt taaccgaggt aaataataac cggagtctct ccggcaacaa
    24001 tttactggtg gttaacaacc ttcagagcag caagtaagcc cgaatgccgc cctttagggc
    24061 ggcatatttt agattatccg gttctgttta aagtcacgca aaaaaccacc ccagcgacgt
    24121 tcatagaatg gcacaatatg ttcggtaata aagtggctaa ttcctttttc cccttttttc
    24181 acctgacaaa tatcgatggg ttcatcgcca ggtaatgtat cggtcgctac acttcccgcc
    24241 gcctgaataa tttcttcgat atcaccatcg gcttcaatgc caataagtaa attaggctgt
    24301 gcctcttcgt tctctttaat tgaacaaata aaagcacgct tcaccggctt aatagtttta
    24361 aataaggtgg tgagtgaatc aatcatttgt gctggcggct ctgcgacttc cgataatatc
    24421 agcgattcac cgccctccag tacttcctgg ctgctcagcg gatttccttc ttcaccaatc
    24481 aacaaactga tttcacgcgg cataaattct ttaccggttg gcagtttggc attaaggaag
    24541 agcgtttcgc caagggtcat ctcaaacaat gtgcgaacgg gcattacgac aaatgcctgt
    24601 tcgtcttcaa ccgcctgttg aagtgcttct aacgaggtaa aaaaaggaat gacgctggtg
    24661 ccgtcttctt tttcccagtg ctgtaaatca agcgcgctat cttcaaccac agcctcgccc
    24721 tgcgccgccg tacccggcac ccagacggtg gattccagta gagtacggaa aaaggccggg
    24781 cggtgcgccg gttcggttgc tgctttttcc agcagatctt caagttcgtt ttttgtttcg
    24841 gacataagaa ccacaattca ttcaacgttc ggggcacaag tgcctgatgc gctacgctta
    24901 tcaggcctac aagggaatct caatctattg gattttcgca attttgtagg ccaaataaga
    24961 cgattacgcc gttaacagat tagcaatcgt gcgcacacca agtcccgtag cacccgcaga
    25021 ccactgttca accggcgctt tacggtaagt cgccgagcag tcgatatgca gccagccttg
    25081 ctgatagttc tcaacaaagt gcgacaggaa gcccgccgcc gtgctcgcgc ctgccgggta
    25141 cgccgcgctt ccggtattgt tcagttcggc aaagttagac ggcagctggc tgcggtggaa
    25201 ctccgccagc ggcagacgcc agaacggttc attctcctgc gcggcactcg ccagcaagcg
    25261 accggcaagc gcatcgtcaa aactgaacag cgcgtgataa tcattaccca gcgcagtttt
    25321 cgccgccccg gtgagggtcg ccgcatcaat gatcagttcc ggtttctgcg cactggcatc
    25381 aatcaggcca tcggccagca ccagacgccc ttccgcatca gtgttcatca cttcaacttt
    25441 tttaccgttg cgataggtaa taatatcgcc cagtttgaac gcgttgccgc tgatcaggtt
    25501 atccgcacag cagaggaaca gcttcacgcg cttgttcagt ccgcgcgtaa tggcaaacgc
    25561 cagcgccccg gtaaccgttg ccgcgccgcc catgtccgac ttcatcgagt ccataaatgc
    25621 ggtctgtttg atactgtagc cgccggaatc aaaggtgatg cctttaccaa ccaggcacgc
    25681 gtacactggc gcttctttat cgccagtcgg gttgtaatcc agcgccagca ataccggaga
    25741 acgttctgaa ccgcgtccga ccgtgtgcag ccccatataa ccttgatcac gcagatcttc
    25801 gcctttggtg atccggtaag tcacacgatc gcccgcgaca ttgctgatca gatcaacagc
    25861 acgctgtgcc agttgcgatg gccccagttc ttccgccggt gcgttgatgg tgtcacgcac
    25921 ccagtcgatg atcatcaggc ggttatccag ttcctgccgt tgggcatcgt ccagatccgg
    25981 ccactccact ttacgtgtgc ctttcggggc tttgtaacct tgccagaatg cccagcagcg
    26041 atccgcatcc cagtcttcac cgcttaactg aacatgcttg atgcccagac cgtcaatctt
    26101 gcgcgccgca cgctggatca gccccagatc gtctgccccg ttcaaatgca gggtaatgcc
    26161 atcattatta atgctgtaag tcgctttttc tccccagcgc gcgtcggcag gttgggtaga
    26221 gagggtaatc ttcatcgctt ctgtcatttt agttatcctt ctttgtaaaa gggccgccag
    26281 ttggcagccc caggattttt ccgctaaatg attcgcgttg caggacaaaa cgcattgcgt
    26341 tttgaacagc gcttgcgctg gccccaaagg ggtgaggcca aaaggccgaa tcacgcggca
    26401 agtgagtgaa gccccaggag catagataac tatgtgactg gggtgaacga gcgcagccaa
    26461 cgcatctgcg gcgtgaagca tgacgcggaa attattcggc ttcgtccagc cagactaaca
    26521 aaatcgcttc gaggattttc tcgttggatg cctgcgggtc gtcgtcgaaa tcttccagat
    26581 cgcaaatcca ctgatgcata tcggtgaatc gaaccgtttt cggatcaaga tcgggatacg
    26641 catcgtacag tgcttcgcca atttcgcggc tatcggtcca cttaagtccc atactaacct
    26701 ctgttaatgc tcacgcgcat ggttgatagt gtaacgcggg atttcgacca ctaaatcttc
    26761 gtcggtaacg cgcgcctggc agcttaaacg gctttccggc tccagtcccc aggctttgtc
    26821 cagcatgtcg tcttcctgct ctgagctttc cggcagcgag tcaaaacctt cgcgaacgat
    26881 gcagtggcag gtggtgcaag cacaggattt ttcacaggcg tgttcaatct cgataccgtt
    26941 acgcagcgca acgtcgagaa tggtttcacc actattcgct tccagaacag cgccatcagg
    27001 gcagagatcc tgatgaggca aaataacaat ctttggcata ttaaacctcg tccacggaat
    27061 ggcctttcag cgcacgacga accgactggt ccatgcggcg agcggcgaaa tcctgggttt
    27121 gtttgtctac gtttttaatc gcttgttcga tagcgtcaac atcatcgccc tgcgccactt
    27181 cactcaggtg agcggcagca tcgtcaatga cctgacgctc tgcggtgctt aacagcgcgg
    27241 catcagcagc cagcgcgccg tgcagacttt ccagcacacg cgccgcttct actttttgtt
    27301 ctgccagcat acgggctttt acgtcctgct cggcatagct cattgagtct ttgatcatcg
    27361 aagcgatttc gctatcggtc agaccataag acggtttgac ctgaatagac gcctcaacgc
    27421 cggtggattt ctccatcgcc gtcacgctca aaagaccgtc ggcatcgacc tggaacgtta
    27481 cgcgaatatg cgcgccgcca gccggtagcg ccgggatacc acgcagtgca aaacgcgcca
    27541 gcgagcggca gtcctgcacc agttcacgtt caccctgcat cacatggata gacattgccg
    27601 tctgaccatc tttaaaggtg gtgaaatcct gggcgcgggc caccggaatc gtggtattac
    27661 gcgggatcac tttctccacc aggccgccca tggtctcgag gcccagcgac agcgggatca
    27721 catccagcaa cagcatttcg ctgtccggct tgttacccac cagaatatcc gcctgaatcg
    27781 ccgcgccaat agcgacgact ttatccgggt cgatagaagt cagcggtgga cgaccgaaaa
    27841 attcgcccac ccgttcacgc accagcggca cgcgggtaga accgccaacc atcaccactt
    27901 ccagcacttc atcagcttct acacccgcgt ctttcagcgc gcgacgacaa gccagtaagg
    27961 ttcgttttac cagtggcgcg atcagttcat tgaattgttc acggctgatt tcgccctgcc
    28021 agcccgcaac gttaacggtc acggagtccg catcgctcag cgcgattttg gctgcaatgg
    28081 cggcatccag cagttcacgc tgaacgcggt tatcgctacg atcaggaatg cccgcctgct
    28141 cgcgaatata atccgccagc agatggtcga aatcatcgcc gccgagcgcg gaatcaccgc
    28201 cggtcgccag cacttcaaac acgccgcgac ttaagcgcag aatggaaata tcaaacgtcc
    28261 cgccgccgag gtcataaaca gcgatcacgc cttcctgacc ggaatccagc ccgtaggcga
    28321 tagccgcagc ggtcggttcg ttaagtaagc gcagaacatg caaccccgcc agacgcgccg
    28381 cgtctttggt gccctgacgc tgggcatcgt caaagtacgc cggaacggtg ataacgacac
    28441 catccagctc gcctgccagg gcttcagttg cccgcgccgc cagtgctttg aggatgtccg
    28501 cagaaacgcg caccgggttc agcagccccg ccgccgtttc aatcatcggc aggccgtttt
    28561 cgctggcctg gaattgataa ggcagatgcg gatagcgttg ctggatatca gccagcgagc
    28621 gccccatcag gcgtttaaca gaactaatgg tgttggcggt atcgagcgcg gcgttagtac
    28681 gcgcgtcata acccaccgaa tgcccttgct gttgatagtg aacaacagac ggcagcaggt
    28741 gacggccttc atggtcggct aacgtttcgg cctgaccgct gcgcactgtc gccaccagcg
    28801 agttggttgt gcccaggtca ataccggccg ccagacgacg ctgatgcggc gcagcactca
    28861 aaccaggttc actaatttgt aataaggcca tgtttagctt ccagaaatta aaaatcgagc
    28921 agtttttctt cgagttgttc ggcactgctt cgcagtttat cgagaaaacg cagcttacgc
    28981 acggtatccg ccgccgcgtc ccacgtctcg ttgtctaact gttcaaccat caactgatgg
    29041 cgggtatcaa acatcttttt cacacgtttg ataaagcttt ccagccgcgc ttcatctttc
    29101 gcctgttcga tctcgtccag ttcttcgcgc agttccagct gttccatcag gaacgcggtg
    29161 tcgcgcacag tatgctgctc gctggcgaga tcaaaaccgt gcaaagaaag caaatattcc
    29221 gcgcgcatta acggatgacg cagcgtttgc caggcctggt taatggttgc agattgctgt
    29281 acggcggcga gttgttccgc ctggcttccg ctggagaatt tatcaggatg atactgacgt
    29341 tgtagatcct gaaaacgcag gctcagcgcc tgggtatcga gttgataaca ggcaggcaag
    29401 ccaaagaggg tgaagtaatc cataacattc tcagggctgc gatccgccgc gataacggcg
    29461 aatcaggtaa aacaaacccc acgcgcaggc gaccacggtg gggttatcgg tatgcgcatc
    29521 aaacgtggaa gctttcgccg caaccacact catctttgac gttcgggttg gtgaatttaa
    29581 acccttcgtt caggccttct tttacgaagt ccagctgcgt accgtccaga aattgcaggc
    29641 ttttgccatc gaccacgact ttcacgcctt tgtcttcaaa cacgatgtct tccggcgtcg
    29701 gttcgtcaac aaattccagt acataagcca tacctgaaca tccggaggtt ctcacgccca
    29761 gacgcaggcc aaaccctttg ccgcggttag ccagaaaggt atttactcgc gctgctgcac
    29821 tgtcgctcag tgtaatcgac ataaccaaac ctcaactctt attttgcttc acgtttgctt
    29881 ttatagtccg caatggcggc tttgatcgcg tcttctgcca gaatagaaca gtgaattttc
    29941 accggcggca gttcaagttc ttcagcaata tcggtgtttt tgatcgcctg cgcttcgtcg
    30001 agagacttcc ctttcaccca ttcggtgacc agggagctgg aagcgatagc agaaccgcag
    30061 ccgtaagttt taaaacgcgc gtcttcaatg ataccttcat cgttgacttt aatctgcaac
    30121 ttcattacgt cgccacaggc cggtgcaccc accatgccgc tgccgacgtt ctcgtcgttg
    30181 ttgtcaaagg aacccacgtt acgcggattc tcgtaatggt cgataacttt ttcgctgtaa
    30241 gccattataa attctcctga ttccgatacc gattaatgat gtgcccattc gatgctgttc
    30301 agatccacgc cctgcttgta catttcccac agcggagaaa ggtcacgcag acgaccgatg
    30361 gatttacgaa ctaactcgat ggtgtagtcg atctcttctt cagtagtaaa acgacctaaa
    30421 gagaaacgga tagagctatg tgccagctcg tcgttcagcc ccagcgcgcg cagcacgtag
    30481 gacggttcga ggcttgctga cgtacaggcg gaacctgaag aaaccgcgag gtctttcagc
    30541 gccataatca gcgactcacc ttcaacgtag ttgaagctga cgttgagaat gttcggcgcg
    30601 ccgtgttcca ggtcaccgtt caggtaaact tcttcgatat ctttgatgcc gttccacaga
    30661 cggttacgca ggccgcgcag acgttccatc tcggtcgcca tctcttcttt tgcgatgcga
    30721 taggcttcgc ccataccaac gatctggtga acaggcagag tgccggaacg cataccgcgc
    30781 tcgtgaccgc cgccgtgcat ttgcgcttcg atgcgtacgc gcggtttacg acgcacatac
    30841 agcgcaccga tacctttcgg gccatagatt ttgtggccgg agaatgacat caggtcaact
    30901 ttcaactggc tcaggtcgat aggcagtttg cccacgctct gggttgcatc aacgtgatag
    30961 ataatgccgc gagcacggca catttcgccg atagccgcga tatcctgcac cacgccgatt
    31021 tcgttattta cgtgcatgat ggaaacgagg atggtgtcgt cacgcatcgc tgcttcaagt
    31081 tctttcaggt cgataatgcc attacgctgc ggtgccagat aggtgacttc gaaaccttcg
    31141 cgctccagct gacggcaggt atccagtacc gctttgtgtt cggttttgct ggtgatgatg
    31201 tgcttgcctt ttttctgata aaagttggct gcacctttga tcgccaggtt gtcagattcg
    31261 gttgcaccag aggtaaagac gatttcacgc gggtcagcgc cgaccagatc tgcaatctga
    31321 ttacgggcga tatcaaccgc ttcttcagcc tgccagccga aacggtgaga acgggaggcc
    31381 gggttaccaa aggttccgtc catcgtcata aactgcatca ttttctcggc aacacgcggg
    31441 tccaccggcg tggttgcgga gtagtcgaga taaatcggta atttcattgc tctataaact
    31501 ccgtacatca ctcaatgcaa ggaaccaggc taccggctgg atgtacgacc gtgtttacgg
    31561 agcgcgatac gccccggcct gattctgaat actttttatt aagcgcgtaa cttaacgtcg
    31621 atcgcgtctt gtgtgcgggt gcgtggcgcg tcgtgagtat gctgacgacc agacacatcc
    31681 agcacttcct ggttattaac cagttcgcct aaagtaatgt tgttgagaaa accggtgaga
    31741 cggtcgctca aatcacgcca cagcgcgtgg gtcaggcatt tatcgccgcc ctggcagccg
    31801 cctttaccct gacaacgggt ggcatctaca gattcgtcaa cggcgctaat cacttcgcca
    31861 acggcgatgc tgctggcatc tttgcctaac agataaccac cgcctggtcc acgtacgctg
    31921 gaaaccagac catttttacg cagacgggaa aacagttgtt ccagataaga aagggaaatt
    31981 ccctgacgtt cggaaatatc agccaacggt accgggcccg cttcagagtt gagcgcaacg
    32041 tcaagcattg cggtcacggc atagcgccct ttagatgtca gtctcatgtc ttacttcacc
    32101 tcaaactcgc ccctgcccgg ggttttttat tgtaaaagtg ggggtattgc atagcagggt
    32161 caagtctgac attcccgagt aaattggtca actatttact tgactgattt agtcgggtat
    32221 ttaaccttca gtgccatttt tttatcgtgg cgtttgtacc tgttgtcgga tgcgacgtga
    32281 acgccttatc cgacctgagg ctctgtgcac tgcaggcctg ataagacgcg ttaagcgtcg
    32341 catcagacaa ccactattat tcggccttat tacctttatt ctgctgctct atagaagcca
    32401 gaatcccgcg caggatattc aattcctggc tttccgggcg cgcacgggta aacaaacgac
    32461 gcaatttatt catcacctgc cccggatggt tttcacgaat aaaaccggtt gccagcagcg
    32521 tttgttccag atgaccataa aaacgctcca gatcatcgac cagcggatac ggcgtctctt
    32581 catgctcgac ttgttcgcca ttttcctgag ttgccagcca ggccatgcgc acttcatagg
    32641 caataacctg aaccgccatc gccagattca gcgaactgta ttccgggtta gcggcaatcg
    32701 cgacatgata atggcatttc tgcaactctt cattggtcaa gccgacgcgc tcgcgaccaa
    32761 aaaccagcgc caccggggta tttgccgctt cagcgacgct tttcagaccg cattcgcgcg
    32821 ggtcgagcat cggccacggc agcgtgcggg agcgtgcgct ggtgcccacc accaggctac
    32881 aaccggctaa cgcttcgtcg agcgtatcga cgatgtgagc attaccaatc acatcgctgg
    32941 cccctgctgc cagggcaatc gcctgggagt caggtttcac cagtggatta accagccaca
    33001 gattggttaa tcccattgtt ttcatggcac gggcaacaga acccatattg ccggtgtgtg
    33061 acgtctccac cagcacaatt cgaatatttt gcagcattgt ctttcttcgt ctaaagatta
    33121 ttcgcgcatc ttatcataaa acgaagacag atgccgatct cgctgctata ctctgcgccg
    33181 ttttcccgtt ctttaacatc cagtgagaga gaccgatgca tccgatgctg aacatcgccg
    33241 tgcgcgcagc gcgcaaggcg ggtaatttaa ttgccaaaaa ctatgaaacc ccggacgctg
    33301 tagaagcgag ccagaaaggc agtaacgact tcgtgaccaa cgtagataaa gctgccgaag
    33361 cggtgattat cgacacgatt cgtaaatctt acccacagca caccatcatc accgaagaaa
    33421 gcggtgaact tgaaggtact gatcaggatg ttcaatgggt tatcgatcca ctggatggca
    33481 ctaccaactt tatcaaacgt ctgccgcact tcgcggtatc tatcgccgtt cgtatcaaag
    33541 gccgcactga agttgctgtg gtttacgatc ctatgcgtaa cgaactgttc accgccactc
    33601 gcggtcaggg cgcacagctg aacggctacc gtctgcgcgg cagcaccgct cgcgatctcg
    33661 acggtactat tctggcgacc ggcttcccgt tcaaagcaaa acagtacgcc accacctaca
    33721 tcaacatcgt cggcaaactg ttcaacgaat gtgcagactt ccgtcgtacc ggttctgcgg
    33781 cgctggatct ggcttacgtt gctgcgggtc gcgttgacgg tttctttgaa atcggtctgc
    33841 gtccgtggga tttcgcggca ggcgagctgc tggttcgtga agcgggcggc atcgtcagcg
    33901 acttcaccgg tggtcataac tacatgctga ccggtaacat cgttgctggt aacccgcgcg
    33961 ttgttaaagc catgctggcg aacatgcgtg acgagttaag cgacgccctg aagcgttaat
    34021 gactctggcg ggtgatgtca ctcacccgcc ctcgcctttc aggcgctatt ccgaaatact
    34081 tcctcaccgc tttactttct ttcgccactc tcccaccatc tttccccgat taatggatga
    34141 taaagaagtc gcaaggaaag gttatggcgc tgccagtgaa caaacgcgtt ctcaaaattc
    34201 tgtttattct ctttgttgtt gccttctgca tttatttaat accgcgcatc gccatcaatt
    34261 ttttctatta ccctgacgac aaaatctacg gccccgatcc ctggtcagcc gaatcagtag
    34321 aattcaccgc taaagatggc actcgcctac atggttggtt tatcccttct gcaactggcc
    34381 ccgctgaaaa tgccattgcc accattattc atgctcacgg caatgccgga aatatgtccg
    34441 cccactggcc gctggtcagt tggttacccg agcgtaattt caacgttttt atgtttgatt
    34501 atcgcgggtt tggtaaatca aaaggcacgc cgtcccaggc cggattgctg gacgatacgc
    34561 aaagtgccat caatgtggtg cgccatcgca gtgatgtaaa cccacaacgg ctggtgctgt
    34621 tcgggcagag tattggcggg gcgaatattc tggatgttat tggtcagggt gatcgtgaag
    34681 gcatacgtgc ggtgatcctc gactccacat ttgcctctta tgcgaccatc gccaaccaga
    34741 tgatccccgg cagtggctac ttacttgatg agagttgcag cggcgaaaat tacatcgcca
    34801 gcgtcagccc gatcccgctt ttactgattc acggtaaagc tgatcacgtt atcccatggc
    34861 agcacagcga aaagttgtat agcctggcaa aagagccaaa acggttgatc ctaatcccgg
    34921 atggcgaaca cattgatgct ttttccgatc gtcacggcga tgtttatcgc gaacagatgg
    34981 tggattttat ccttagcgcg ttgaatccgc agaactaacc catgatcgct agcacgataa
    35041 tcattcacaa aaccacctta agacatgcta atccactggt cagaacagtt taagatgaga
    35101 aaaattctgt gacgcttgcc aacatttctg atgattagca ttcccttcgc catttccatg
    35161 agcaaacttt agctattctt atcaattatg cttatgggag atctacagat gatgcctacg
    35221 cttgctccac catctgtcct ttcggctccc cagcgccgct gccagatctt gctgacactc
    35281 tttcagccgg ggttgaccgc caccacggca actttcagcg agcttaatgg tgtggatgat
    35341 gatattgcca gtcttgatat cagcgaaaca ggacgtgaga tcctgcgcta tcatcaactc
    35401 acactgacta ctggttatga cggtagctac cgggttgaag gtacagtgct taaccaacgt
    35461 ttgtgtttat ttcactggct acgacgtggt ttccgtctgt gtccgtcatt tattaccagc
    35521 catttcaccc ccgccctgaa gagtgaactg aagcggcgcg gaattgcgcg taacttttac
    35581 gacgatacca atctacaagc gttagtgaat ctctgctccc gacggctgca aaaacgcttt
    35641 gaaacacgcg atattcattt cctgtgtctg tatctgcaat attgtttgct gcaacaccac
    35701 gctggaatta cgcctcagtt taatccgctc caacgtcgct gggcggaatc ctgccttgaa
    35761 tttcaggtag cgcaggaaat tggacgccac tggcagcgtc gggcgctcca gcctgtaccg
    35821 cctgatgagc cactgttcat ggcattactt ttttccatgt tgcgggttcc cgatccactg
    35881 cgggatgcgc atcagcggga cagacaactg cgtcagtcta tcaaacgtct ggtaaaccat
    35941 tttcgtgagc tgggaaatgt tcgtttttat gatgaacagg ggttgtgcga tcagctttat
    36001 acccacctcg cccaggcgtt aaatcgcagt ttgtttgcca tcggtattga taacaccctg
    36061 ccggaagagt tcgcccgcct gtatccacgc ctggtgcgca ccacccgcgc ggcgctgacc
    36121 ggatttgaaa gtgaatacgg cgtccatctt tctgatgagg aaagcggtct ggtcgcggtg
    36181 atttttggtg cctggctaat gcaggaaaac gacctgcatg agaaacagat tattctactg
    36241 accgggaatg atagtgagcg agaagcgcag attgagcagc agctacgcga actaacgtta
    36301 ctgccgctca atattaaaca tatgtcggta aaggtatttt tgcagacagg cgctccgcgc
    36361 agcgcggcac tgattattgc tccttatacc atgccgttac cgctcttttc accaccgctg
    36421 atctatacgg acctgacgtt gacaacacat caacaggagc agatccgcaa aatgctcgaa
    36481 tcagcatgag ggaacaactt tcgggcgcaa aaacattgcc ggaagcgcca ccagcgccat
    36541 aacccagaac acgccgtggc ccagatattg atacaggaaa ccggcgaaaa cggtcatgat
    36601 cgcaatactg ccgcccatcg cgacggcaga gtaaaccgcc tgtaaacgga tgacttcgct
    36661 accctggcga gcggcaatat agcgcatggc ggccaggtgg cagaccgtga aggtgccaca
    36721 atgcagaatt tgcaccacta tcaaccacgg caacgccgta gttgctccca taatgcccca
    36781 gcgcactacg ccgcaaatcg ctgagatcaa cagcatatcg cgtgcactac aacggcggaa
    36841 aagtttatta ctcagcgcaa agataatgac ttccgccacc acgcccagcg accacaaata
    36901 gcccaccgcc gaggccgagt agccagcagc ctgccagtaa atggcgctaa aaccgtaata
    36961 ggccgcatgc gccccctgca ataaacaaac gcaggccaga aagcgccagt tctggcgaac
    37021 caacgccaac cacgcagacc aaccggtgct ctcctgctgg cggcttgccc cttgtggctg
    37081 aatcgtcgga cggatgagaa agccgagcag catggatgcc acgcccaacg tcaacagcgc
    37141 gaggatcacc cgataatcaa acatactgac cagtttgccc gtcagcgccg agccaatgac
    37201 aaacgctacc gagccccaca gtcgcacttt gccgtaatca agcgggaact gcttttgcca
    37261 cgtattcgcc agtgcatcgg tcaacggtac cagcggtgag aaaaagaggt taaagccaat
    37321 catcaccagc atcagccacg ctacgtgcgc ccccgcccag aatgcgacag caaagagaag
    37381 tgtcagcagt gccagcacgc gcaaggcgga aatcaggcga gaaggatcgc tgacgcgggg
    37441 cgcgatgagc aaactcccga gaaaacgggc aaccaaacct gcccccaata acaggccgat
    37501 ggtttctggc gttaaaccaa tccccttaag ccagacgctc cagaaaggta gaaaaatgcc
    37561 gtaactaaaa aagtatgtga aatagccgag cgccaaccag cgcgtggatt gcaaaaccat
    37621 gagtccctcc cgtcaggagg cgttagtctg gggttaatcg aatgttggtg caagttgaaa
    37681 aataagaata ttcgagcctg cttcccgatg tctgtaacac cagttcacaa gcactaaaaa
    37741 actgcgttcc gcgacgcatg ttgcaaaaat aatgcgtaat tataccgtta cgcttgccaa
    37801 acgttcctgc acaatggcga tgaaatcgcg taatgccggc ttcatctccc ctttcttcca
    37861 cgccatcagt aaagcaatgg aaggcacatt tccggcaatg ggtcgaaata ccacctggcc
    37921 ggtattaaaa ttattcatat aaccgggtat caaagtgacg cccagcccca tccccaccag
    37981 attcatggtc accagaatat tcgttgccac ctggacgata tttggctggc tgttttcttg
    38041 cgcaaaccac gctttaacga tcggcgcaag cgaaccggaa tacgctggat cagtactgac
    38101 gaaattcacg ccatccagtt gcgcggcagt gatctctttt tcatgtgcta aagggtgatc
    38161 aaccggtaac accaccacta atggttcgtc aaaaagctcc agataatcaa tctccgggct
    38221 ataaacagga tggcgcatta agccgacgtc gagttcacca cggcgaattt tttcttcctg
    38281 ttccgtggtg attaaactca ccagctcaat taaggtgtct ggctgtctga gacgaaacat
    38341 cggtaatact tttggcagta aattcacttc cgccgatggc acaaagccaa tggttaattg
    38401 tctgtcttcc tgaacaattt tccgcgcccg taatttggca ttttccgctt gttcgaggat
    38461 tgccagcgcg tcctggagaa aacattctcc cgccgccgtc aacgcgactt tgcgcttatc
    38521 cctcaccagt aacggaacac cgacacagtt ttcaagatcg cggatctggc tgcttaacga
    38581 aggctgtgag gtatgcagtt tttccgccgc acgggtaaag ttcagtgcct gcgccactgc
    38641 gacgaaatag cgtaaatgcc gtagttccat caccttcccc ttgttatcga aaaaacgtct
    38701 cagctggtag aaattaacta tttcacatat tagcaaccaa ccagcaacat ccttatagca
    38761 caaaaataga aggtcaatac atcttatctt tcaggattaa aaaatatgac cacaccctca
    38821 gatttgaaca tttaccaact gattgacacc caaaacggtc gggtcactcc gcgtatttat
    38881 accgacccgg atatttacca actggagctt gagcgcattt tcggtcgttg ctggctattt
    38941 ctcgcccacg aaagccagat cccaaaacct ggtgatttct ttaacaccta catgggggaa
    39001 gatgcggttg tcgtagtgcg tcagaaagac ggcagcatta aggcgtttct caaccaatgc
    39061 cgccaccggg ccatgcgtgt gagttatgca gattgcggca acactcgcgc ctttacctgt
    39121 ccgtatcacg gctggtctta tggcattaac ggcgagttga tcgatgtacc gctggaacct
    39181 cgcgcctacc cacaagggtt gtgtaaatcc cactggggac taaacgaagt tccttgtgtg
    39241 gagagttata aagggctgat ttttggcaac tgggatacca gcgcaccagg cctgcgtgat
    39301 tacctgggtg acattgcctg gtatctggat ggcatgctgg atcgtcgcga aggcggcacc
    39361 gaaattgtcg gcggtgtaca aaagtgggtg atcaactgca actggaaatt cccggcagag
    39421 cagttcgcca gtgaccaata tcatgctctg ttcagccatg cttctgccgt tcaggtatta
    39481 ggggcgaaag atgatggcag cgataagcgc ctcggtgatg gacaaaccgc ccgtccggta
    39541 tgggaaaccg ccaaagatgc gctgcaattt ggtcaggacg gtcacggtag cggtttcttc
    39601 tttactgaaa aaccggatgc taatgtctgg gtcgatggcg cagtttcaag ctattaccgc
    39661 gaaacctatg ccgaagcaga acaacgttta ggtgaagttc gcgccctgcg tctggcgggt
    39721 cataacaata tattccccac gctttcatgg ctaaacggca ctgccacact ccgcgtctgg
    39781 catccgcgcg gccctgatca agttgaagtg tgggcgttct gtattactga caaagccgcc
    39841 tccgatgaag ttaaagccgc tttcgaaaac agcgccactc gtgcttttgg tcctgctggt
    39901 tttctcgagc aggatgactc ggagaactgg tgtgaaatcc agaaattgct gaaaggtcac
    39961 cgcgcccgca acagcaaact gtgtctggaa atggggcttg gtcaggaaaa gcgtcgcgac
    40021 gacagcattc caggcattac taactatatc ttctcagaaa cggccgctcg tggaatgtac
    40081 caacgctggg ccgatctcct gagtagcgaa agctggcagg aagtgctcga taaaaccgcc
    40141 gcttaccagc aggaggtgat gaaatgagtg cgcaagtttc actggagtta catcaccgca
    40201 ttagccagtt tctctttcac gaagccagtc tgctggatga ctggaaattt cgtgactggc
    40261 tggcacagct cgacgaagag attcgttaca ccatgcgcac cacggttaac gcacaaacgc
    40321 gcgaccgccg caaaggcgtt caaccgccga caacctggat ttttaatgac accaaagacc
    40381 agctggaacg gcgaatcgcc cgtctggaaa ccggcatggc ctgggcagaa gaaccgccgt
    40441 cacgcactcg tcacttaatc agcaactgcc aggtaagcga aaccgacatc ccaaacgtat
    40501 ttgccgtgag ggtgaattat ctactttatc gggcacaaaa agagcgcgat gaaacattct
    40561 atgttggaac gcgtttcgac aaagttcgcc gtctggagga cgggaactgg cgcttgctgg
    40621 aacgggatat cgtcctggat caagcggtca tctcctccca taacctgagt gtactgttct
    40681 gatgaatcga atttatgcgt gtcccgttgc ggatgtgccg gagggtgagg ctctccggat
    40741 cgatacctcg cccgtcatcg ccctgttcaa cgtaggcggc gagttttatg ccattaacga
    40801 tcgttgcagc catggtaatg cgtcgatgtc agaagggtat ctggaagatg acgccacggt
    40861 ggagtgcccg ctgcacgccg ccagtttttg cctgaaaacg gggaaagcgt tatgcctgcc
    40921 cgccaccgat ccgctaacca cttatccagt acacgttgaa ggtggtgaca tcttcatcga
    40981 cttaccggag gcgcagccat gagcgatctg cataacgagt ccatctttat taccggcggc
    41041 ggatcgggat taggcctggc gctggtcgag cgatttatcg aagaaggcgc gcaggttgcc
    41101 acgctggaac tgtcggcggc aaaagtcgcc agtctgcgtc agcgatttgg cgaacatatt
    41161 ctggcggtgg aaggtaacgt gacctgctat gccgattatc aacgcgcggt cgatcagatc
    41221 ctgactcgtt ctggcaagct ggattgtttt atcggcaatg cgggcatctg ggatcacaat
    41281 gcctcactgg ttaacactcc cgcagagacg ctcgaaactg gcttccacga gctgtttaac
    41341 gtcaacgtac tcggttacct gctgggcgca aaagcctgcg ctccggcgtt aatcgccagt
    41401 gaaggcagca tgattttcac actgtcaaat gccgcctggt atcccggcgg cggtggcccg
    41461 ctgtacaccg ccagtaaaca tgccgcaacc ggacttattc gccaactggc ttatgaactg
    41521 gcaccgaaag tgcgggtgaa tggcgtcggc ccgtgtggta tggccagcga cctgcgcggc
    41581 ccacaggcgc tcgggcaaag tgaaacctcg ataatgcagt ctctgacgcc ggagaaaatt
    41641 gccgccattt taccgctgca atttttcccg caaccggcgg attttacggg tccgtatgtg
    41701 atgttggcat cgcggcgcaa taatcgcgca ttaagcggtg tgatgatcaa cgctgatgcg
    41761 ggtttagcga ttcgcggcat tcgccacgta gcggctgggc tggatcttta aggaagcacg
    41821 atgaaagaaa aaacgatcat tattgtcggt ggcgggcaag cggcggcaat ggctgcggcc
    41881 ttgctacgcc agcaagggtt caccggtgag ctgcatctgt tttccgatga gcaacatctt
    41941 ccttatgaac gccccccgct ctcgaaatcc atgttgctgg aagattcccc acagttgcag
    42001 tctgtgttac ccgctcactg gtggcaggaa aacaatgttc atctgcattc cggtgtaacc
    42061 atcaaaacat tgggccgcga cacacaagag ttagtgttag ctaacggcga aagctggcac
    42121 tgggatcagc tttttatagc aactggcgcg gcagctcgac cgctgccgtt gcttgatgca
    42181 ctgggagaac gctgctttac cctgcgccat gccggtgatg ccgccagact gcgagaagtt
    42241 ctgcagcccg aacggtcagt cgtgattgtc ggtgccggaa ctattggtct ggaactggct
    42301 gccagcgcca cgcagcgcgg atgtaaggtg acagtgattg aactggcggc aaccgtcatg
    42361 ggtcgtaatg caccaccgcc cgtacaacgc tatcttttgc agcgccatca gcaggctggt
    42421 gtgcacattc tgctcaataa tgccattgaa catgtggtcg atggtgaaaa cgtagaactg
    42481 acgctgcaaa gtggcgaaac gcttcgggcc gatgtggtga tttacggtat tggtatcagc
    42541 gccaacgacc aactggctcg cgaggccaac cttgatacta ccaatggcat tgtcattgat
    42601 gaggcttgcc gcacctgcga tcccgcgatc tttgccggtg gcgatgtggc aatcacccgt
    42661 cttgataatg gtgcactaca tcgctgcgaa agctgggaaa acgccaataa ccaggcgcaa
    42721 attgccgctt ccgcaatgtt ggggctaccg cttccgcgac tgccgccgcc gtggttctgg
    42781 agcgatcagt acagtgataa cttacagttt attggtgata tgcgtggtga tgactggctt
    42841 tgtcgtggca acccggaaac tcagaaggcg atttggttta atctgcaaaa cggcgtgctt
    42901 atcggtgcgg taacgctgaa tcaggggcgt gagattcgct taatccgcaa atggatccag
    42961 agcggcaaaa cgtttgatgc gaaactgctg acagatgagc acatcgcgct taaatcactg
    43021 taaccaggat aattagcgaa tatctcaatg cctggggcgt ggcgaggtgc cagagtgtgt
    43081 attacgttta aatcacatta tcttgcaaag ggattggtta tgaacacact acgttatttt
    43141 gattttggag ctgcccgccc cgttttgtta ttaattgccc gtatcgccgt ggtcttaatt
    43201 ttcattattt ttggttttcc caaaatgatg ggttttgacg gtacggtcca atatatggcc
    43261 tcgctgggcg cgccaatgcc gatgctggca gcgattattg cggtagttat ggaagtgccc
    43321 gccgcgatat taatcgtgct tggctttttc acccgtccgc tggcggtgct gtttattttc
    43381 tacacgctgg gtacagcggt aattggtcac cattactggg atatgaccgg cgatgcggtt
    43441 gggccaaata tgattaattt ctggaaaaat gtcagtatcg ctggcgcatt cctgctgttg
    43501 gcaattaccg ggccgggggc aatttctctc gatcggcgtt aggaaagatg ccggatgcgg
    43561 cgtgaacgcc ttatccggca ttttataaat tacagtaacg caacccgcaa cgacatctct
    43621 gaagttgttg attcccctgg tgcaagcgca atgagatcac ccccttccgg gcgatgatgg
    43681 tcatccggcg catggctcat cggttccaga cagaagaaat caaacgcata tcctttatca
    43741 aacgccgggt cggaaacaaa gatgaaataa catggtgcag gtggcgtcgt ttccatgatg
    43801 atggcataac cctcctgcgg ctgctcgatg cgggcctgac cattccatcc ggcaaagcca
    43861 ttgttcaccc actggtgcgg caacggcgcg agctggttga aatccagctc ctgcggtagc
    43921 tgctcgcaaa actcacccgc cagccactgc tcccgctcca gccagtaacc gctcgcctgc
    43981 gcctgaatcc gcgtttgtgg tgacaacgga aaataaggat gccagccggt accaaatggc
    44041 agcgtctctg tcccttgatt ggtgacagag agcgtcaccg tcagcgtatc tgccgttaaa
    44101 tgaaacgcct gacttacccg atagtgataa acaccgctgc gatgttcata caccagacac
    44161 aagctatctt cgctgcgcga gacacattgc cactgcccca gccagccgtc gccgtgcaga
    44221 tagtgcgcat cccactcaac attcggttgc agttgatact cacgcccctg ccagacaaaa
    44281 cgattgccgc tcacccggtt tgcaaacggc accagcggaa aacaggaggc atcggttgcc
    44341 acaccgcttt ttttaccggg acgtaagagc ggcgtcgcgt cacgccagaa gccttcgatg
    44401 acgccgcccc ggtcagaaac gtccagctct agcggcccgt gagataacgt atagatggtc
    44461 atccgctgac tccttaatcc gggaagttaa tcacaacttt cccgcattta ccgctcgcca
    44521 tcagcgcata ggcatctcct gcctgttcca gcgtgaagcg atgggtaatg gcattacgcg
    44581 gccacagttt ccagtccgtt aaatcgtggg cgcatttttc catatggaac agactggtca
    44641 cccaggagcc aataatccgc cgttgatgat gcatcagatc ggcgctaacc tcgaactcca
    44701 cttttccggt ttccccgatg taaaccaccc gtccccagtc agcagtggat tgcaacgcca
    44761 gtaagcgacc tgcggcatta ccggaacaat cgagcgcaac atccgcgcca ccgtgggtga
    44821 gttcggcgat aatctgcggc agaccttcgg tggttgctaa atagccgtga tccatcaccc
    44881 ctaactgttt cgccatcgcc agacgttccg gcagcatatc aacgccgatg atcctttttg
    44941 caccgcgacc tttcgccagc atcatcgcca tcatgccgac cggccccaga ccgaccacca
    45001 gcacgttatc gctgccggaa acttcgccgc gcaaaatccc ttcataggcc gtgccaacgc
    45061 cgcaactaat aaacgcacca tcttcgtagc tcagcgcatc cggcaggagg atcagatctt
    45121 tttcttccgc cagcaagtat tcggcatgac cgccgtcacg ctgccagccg taagccgctt
    45181 ttccttcgcc agtacaagaa ataggaaaac cgcgacggca gttcgggcaa aaaccacagc
    45241 cagaaatgtg atacaccagc acgcggtcgc cctctttaaa atggcggcag ccttgcccca
    45301 tcgccacaat ttgcccgcac ggttcatgac cgttgataaa tccctggtat aacggtttat
    45361 cgggtgccgc tgccgtggca cggtgttgat gatagatata gtggacatca cttccgcaaa
    45421 tcccggagga tttcattttg atcagtacct ggttaatccc cggcgtcggc accgcaactt
    45481 cccgcagatc gacggtcgaa tttcctggta aataagctgc cagcatcgtt ttcataaatc
    45541 cctcataaac aatacgatta attttcatcc ctgccgcacc cgcgccaggg gcgttaaggt
    45601 ttagcgtttc gctttactgc tacgctgggt cagcaagata ttcgccagca ccgccaccac
    45661 gatgatgacg ccgcgcacca cctgctggaa aaaggagtta atcccgagca gcaccagacc
    45721 gttaccgatt agcgtaatca ccaacacacc aagcaatgta ccaaacaggg aaccgcgacc
    45781 accggaaagc gccgtaccgc caaccacgac cgcggcgatg acgtcaaact ccagaccgtt
    45841 tgcggcacct gcgttaccgg aaccgaggcg cgccgccaac aaaatgccgg tcaccgctgc
    45901 taataatccc gaaagggtaa aaataagaat gcgtacccga cgaacgttaa taccgcacaa
    45961 ctgtgctgca gtggcattac cgccaacagc aaaaaccgag cgcccgaagg cggttttgcg
    46021 gctaatgaac acaaacagcg caaataacac catcatgatc agcgcggata ccggcacacc
    46081 gagaaattgt ccccccagcc agtccagcac ttcgttttcg tcaatcggca ctggcaaggc
    46141 gttcgtcata aacagcccca ttccgcgcag ggcgctccac agccccagcg tggccacgaa
    46201 acttggcacg ttaaacacgc cgcgcagcac cccggcgagc gttcccatca gcgcgcctaa
    46261 cagcaacacc agcagacaag caatcgccaa cggaacttca aattgcagca aaaatgccag
    46321 gcacaccgag acaaaagcca ccatcggccc aacgctgaca tcaatttcac cggagataat
    46381 aatcagcgtc atcgcccagg cggcaatccc aatggtggcg gcatcgcgca gcacgttcat
    46441 ctggttattc aatgagataa aacccggcgc gttcagagaa aagaccagat aaagaatggc
    46501 gatcaccacc agcaaaccga tctcattaat atggcgactg acaaattgtt tgagcgagac
    46561 gctcttgccc tgcggcaatg gtaatgacga agcagacatc ttcaggttcc tcggtaaaat
    46621 tcatcagtgc acagacagaa tggcggacat cagctcatcc acattgaccg gcgagtgaaa
    46681 ctcctgcgag aacgtaccgt gctgtaataa caggatgcgg tcacacacca gcggcaactc
    46741 ctccacttca ctggagataa acaccacgct ttttccttcg gcagccagct cgcggacaat
    46801 acggtaaatc tgctgtttgg cttcgatatc gacgccacgc gttggctcgt cgagcaacaa
    46861 aatctggctg gcggcataga cccaacgacc gatcaccact ttttgctgat tgccgccaga
    46921 aagcgtgccg atgggcgttt cgctactggc ggccttgacc gtcatccgct gcatcacctc
    46981 ttcggtcagg cggcggatgg tggaccattg cagcacaccg ttggcgctga ttttttgccg
    47041 attggtcagc actgtatttt cgtcaacgcc cagccaggga atgatccccg cttctttgcg
    47101 gttttctggc gtatagccaa tgccgcgttt cagcatgtcg ccgtaatcgg ggcgcgtgat
    47161 tttctcgccg ttgataacaa tttcgccctg ttcatacgtc tccagcccaa ctatggcttt
    47221 caacaattca ctgcgccctg cccccagcag gccagcaatg ccgagtactt cgccacgacg
    47281 gagcgtaaag ctgatatcct ccagcttggg cttatggcgt aacgcacgga cttccagcac
    47341 ggcctgatcg acaatttcct gaggggaaac cggcgcaata tcaacgtgat cgcgcccgag
    47401 cattagcgac acaatatgat gcgtggaggt gttttcgagc atcacatcgc ccgccacctg
    47461 accatcgcgc ataacggtgg cacaggaggc aatgcggcgg atttcttcca tccggtggct
    47521 gacataaatc accgccacgc ccagtgccga catctttttc accgcgctga tcaccagttc
    47581 aacttccgca ctggcaagcg aactggtagg ttcatcaaga atgaccacgc gcggctcgcc
    47641 cttcatcacc cgcgcaattt ccaccagctg cttttgtgcc gggcttagcg ttgaaacaag
    47701 ttgttcaggg ctaacgtcaa cgcccagcgc ctgtaagcaa cgttgggcat cctgcgccat
    47761 ttgcaggtaa tcaatcatgc cgttgcggcg gggccactga ccgaggcaga ggttttccgc
    47821 cacagtcagt ccctccacca gacttaactc ctgataaacc gcacgaaccc ccagttcagc
    47881 ggcacggcga gtcaacgtag cttcgtcacc ttccagtcgc gtctcgccaa tccagatatc
    47941 accgctatcc ggacgttcac tgccggtaag cattcgaatg agggtcgatt tgcccgcgcc
    48001 gtttttgcct aacagcgcac gaacttcgcc tttattgagc gtgaagttaa cgttatccaa
    48061 cgcaacgacg ccgggataac gcttatttcc tgccaccact tttgctaccg ggactgcctc
    48121 tgttgccgtg aacataggga cctctgcgaa tcagcgatta gggcagacca tcaacgtgcg
    48181 ttgccagcca ctgtttaccg tcttccgttt tggtatagag atcgattggc acctgaatca
    48241 ctttttcacc gtcggcttgt ttattgataa ccttcaaagt ttgcgcgaaa acagcattgc
    48301 ccattttctt accggaaata tccaccaccg ctttcagcac ctgattgttt tccagctcct
    48361 gagcaatttc agtggtcata tccgaaccga aaacagcaat ttttccggcc tgattttgat
    48421 tacgtaccgc ttttaccgcg ccgagtgtcg caccgcccga ttcccccata atggcgttga
    48481 gatccgacgt ggagataatt agtttttcac caacggaaat cgctttatct aaaacagtcc
    48541 cttcctgatt agcgacaatt tgcgcgccgg gaacgcgggc ttttaatact tcttcaaacc
    48601 ctttacgtcg ttgcacacaa acttcaaagg cttcgcaatt gatgacggca attttcggct
    48661 ggtcaatttt gttggcaata aaataatcgg cggcagcgtt acccagtttt ttaccaaatt
    48721 ccagcgggtc accaaccaga tatgccgaga catatttatc gacacccttt tgattaatac
    48781 aggtgttgta gcaaatcacc ggaatacccg cttcactggc gcgacgaacg gtacggctac
    48841 tgccattttc agatactgcc gataaaataa tggcatcgac attacgcgcc acgagggtat
    48901 ctacaaaagt actttctttc gaaatatcgc cctgggcgtt agtttcaatt aactgtacct
    48961 gtactgaaga atcttttgcc gcatcctgaa cgccctgacg cacgccagcg taatatccct
    49021 gggtatcaag gtatattgca ccaatggtca tttctttatc cgctgccctg gcaaatagag
    49081 cgctacctaa tagcgtagcc attaataata gattacgggt tgttctcatt tttttaggca
    49141 tagtgcctcc tgtagggttt ttatttacaa cggcttattc taattatttt gtgatgagcg
    49201 gcagcgcgtg ccgctcaaat attacagtta ataaggttta gtgaacgcgg ttaaaaataa
    49261 aaggcatcac ggtggtgaat aatgctgctt tcggccaggc cggattaatc gccgtcaatt
    49321 cagcgcgagc agattcatat tgcgcaacct cccctagtcc cgcggacgcc agcatcgcca
    49381 ccatcaggca tttttcttta tgctgctgtt gtaaatcgcc atacagcgac aacaggtcag
    49441 gttgcgagac ggcaaagaaa tccgcttcga tactggtttt cgccatctct tgcgcccaca
    49501 gtttcatttc actaaacagt tgctgtgcgg tgtgttgttc gcccagcagt cgcagcgcca
    49561 ttccttgcca gaagagataa tcaaccggct gatcgttgta ataactgtgg atgttaatgg
    49621 tgcgatcgcc ggtcgccgcc agacgtaaac aacgcgtcgc ttcagtttca tcgccctgcg
    49681 cgttggcgca tatcgcctgc cagaaccaga tgtcgttatc agtttgcccc ggtaaacggc
    49741 cttcgcttaa attctccgga taatgcagcg cggcatgaag cagttcgctg gcctgctgcg
    49801 gctctctggc atcaagatgc tgccaggcgc gtaataactg gttgaggata aactgactgg
    49861 tgatcttccc ttccccgcct tcccacgggt ggaatttgcg cgtggcgaga atgtccgccg
    49921 ctttgtctgc ctgacccgtg agatgccaca aattgagcag ttctgcggtc atgtcgtcgc
    49981 gtttcagcgc aatttccaga ttattttcca gacgcgccag tcgtttctcc ggtgtggctc
    50041 cacttaactt atccagcaaa tcccgttcga aaagcagacg tgcatcctgc ggcgcaagct
    50101 gataagcatt atcaagataa cgcgcggcca gctcataatc gtgttgctta ttccacgcat
    50161 ggatcgctaa cccgcgccaa ccgtcggcaa actccggcga catctctacg caacgttgcc
    50221 ataaagtaat ggctttgttg tagctgcgct tgttgtagta gaagcaggcc agtaaatgac
    50281 gggcaaacca gcactcttca atactctcca gcgccgccac ttcttccagc gtattcggga
    50341 aacggacaaa ctgcgggaag acatcaatgg cttttgcgac cagttcgccg cgttcggctt
    50401 tcggcagcaa gctggcttgc aggtaaagcg gcagcgtgcg ctggcagtcc agtgcgttca
    50461 gcatctctgc cgccagggtg ggcattcccc agttaatcag ttgcccggcg gtcatcaggg
    50521 cgttaacgtc gcgtccctga cacagaccgc gccactgcgc gagggctgat tcgctacgac
    50581 catcgaacca gttcagccac cacagagtgg cgttcagcgg ataatcgcgc agcagtttct
    50641 cgcgctgcaa acgcgcgttg tcctgacgac cacttaacac cagcagcaga ttatgcaggc
    50701 aaagcacttc ctgattggtt gggcaggcgc gaagactttg ttggcaaaaa tccagaccag
    50761 catcgaagtg accattacgc gtcgccagtc gtgccagacc ataatagccg cctgctttac
    50821 tattgccgct ccagaccgca cgccagaaat cctcttcggc ttgttgatat tgtccctgac
    50881 gttcgtaagc actggcgcga atcaaactgg cctgtccgca ctgcggattt ttgttcagcg
    50941 catgtgcgcg tttcagcgcc tgagtggcat acgccaccgc ttgcgggaaa tcggcgcggt
    51001 tatattccag catcgccagc gccaggttac agcgataatc cagcggatcc agcgccacgc
    51061 cgcgcaggta gtaatcgaac ggtgaacggc tggcgtgatg atattgctcc agatgctgac
    51121 cgataaacca ggcttcatct gtactggtaa tgtcttgtgc tgccagtggc gctttggcga
    51181 cgtccggcaa cggcaactct tgcggctgat gttcctgata actcagtatg atattgccgt
    51241 cggcatcgga gagttcaatg gtcagccttt caggattgat accgtgcaac acgccctgga
    51301 tggcggtcgc tggtgtcagg gccacggcat catcgagtaa cgcgttgcat ttgccgattt
    51361 cgcggatcgc cagacggtat cctttcaacg gagagatggc atacagcccc cactcaatcc
    51421 cccgctcact acgctggagt tttatcaccg catcgcggga ggcattttgc acaatgccca
    51481 gagaatgata aggcaggaaa tactgctcga aacgcttctc ttcgtacgca tcaagccagg
    51541 taaaatcagg ctggttatcg gcaaaaatac cggtcatcag ttcgatatac gggccgttat
    51601 tgtcggtcag actcttatcc cacgcctggc caaattcact gtgtccccaa ctccactgtt
    51661 ttttacctgg cgcaatatgg tggttggcaa cgtgcagcaa accgccatct tcatcgtgac
    51721 accacgcgcc aacaaaatcg tactgtgatt tttcagccat atatgaggtt ggaacaggca
    51781 catttttata gcgagaaatg tccactccag cggagtagtc cactttgtag taagtgccgg
    51841 tggcgatggg gaaagcagag acggcccgtt tgccgtgatc aaacaccgca gtcacatccg
    51901 gtgggaaaac gctctgatgc ccttcacccc ctttcactgc cgggttggcc caccacaaga
    51961 aatgacgcgg cgtggcgttg ccgttataga cgcggctggc gatttccagc gccgcccggt
    52021 caggacgcag ggtgaaacct gtcatcacct gtaaaccatg catcggctcc gtttcgccta
    52081 cccacacggt ttgtgcgccg tcttcatggg cttcgagggt gaaatcaacg ggcataaagg
    52141 tggtcgggcg atggtgttgc ggccagttaa actcaatccc gccggagatc cacggtccca
    52201 gcagccccac cagcgccggt ttaatgactt cattgtgata gacaaaatcg cgctgtttca
    52261 ctttatccca cgcgcgatgc acccgaccgc ccagttccgg caggatcatc actttgatgt
    52321 agtcgttttc cagccacacc gcctgccagg atttcagggt tttctgctcg ctcagcgtat
    52381 cggtcacgcc gtagggataa accgcgccgg acgatccctg ataaacgcga ttttccagga
    52441 acatgggatg tatatcctgc ggcccggttt cataggtcgg gatctcaacg cgctcttgcc
    52501 acacttttac tggagtcatg gtgccctcaa tattaacaag acatactgaa ttaaaagatt
    52561 tgtggcagtg tattgaacaa tctggcaatg ttttcgcgga ataatcacgc aattaactaa
    52621 acaaggttta gtgaagatga gagcctgcat taataatcaa cagattcgcc accataacaa
    52681 atgcgtgatt ctggaactgc tgtaccggca aaagcgcgcc aataaatcaa ccctggcccg
    52741 gctggcgcaa atttcgattc cggcggtcag taatattttg caggaactgg aaagcgaaaa
    52801 acgggtggtg aatatcgacg atgaaagcca gacgcgcggg catagtagcg gtacatggct
    52861 gattgcgccg gaaggtgact ggacgctgtg cctgaacgtg acgcctacca gtattgagtg
    52921 tcaggtcgct aacgcctgtt taagtccgaa aggcgaattt gagtatttac agattgatgc
    52981 accgacgccg caggcgctgc tgtccgaaat cgaaaaatgc tggcatcgcc accgtaaatt
    53041 gtggccggac cgtaccatca acctggcgct ggcaatccac ggtcaggttg atccggtgac
    53101 tggcgtgtcg caaaccatgc cgcaagcacc gtggacaacg ccggttgagg tgaagtatct
    53161 gctggaagag aagctcggca ttcgggtgat ggtcgataat gactgcgtga tgctggcgct
    53221 ggcggagaaa tggcaaaata attcgcaggg ccgggatttc tgcgtgatca acgttgatta
    53281 cggtattggc tcgtcgttcg tgattaacga gcaaatttat cgcggcagtt tgtatggtag
    53341 cggacagatt ggtcacacca tcgttaatcc ggatggcgtc gtctgcgact gcggacgtta
    53401 tggctgcctg gaaaccgtcg cctcgttaag cgcattaaaa aaacaggcgc gggtatggct
    53461 aaaatcacaa ccggttaata ctcagcttga tcctgaaaaa ctgacaacag cgcagttaat
    53521 cgctgcctgg cagagtggag aaccgtggat caccagctgg gttgatcgct ctgccaatgc
    53581 cattggtttg agtctgtata acttcctcaa catcctcaat attaatcaga tttggttgta
    53641 cggtcgcagt tgtgcctttg gtgagcactg gcttaatacc attattcgcc agacgggatt
    53701 taacccgttc gaccgcgacg aaggaccgag tgtgaaagcg acgaaaattg gctttgggca
    53761 attaagccgc gcacaacagg tgctggggat tggctatttg tatgttgagg cgcagttacg
    53821 gcagatttga tggcgcgata acgtagaaag gcttcccaaa ggaagccttt tttcaacaga
    53881 ttaaccgttt cgcttatgcg taaaccgggt aacgtgcgca gatgtcgaga actttacctt
    53941 tgatgcgctc gataaccgct tcatcattga tgctgtccag cacgtcacac atccagccag
    54001 ccagttcttt cgcttctgct tctttgaagc cgcgacgggt aatcgccggt gtacccacgc
    54061 gaataccgga ggtcacaaac gggctcttcg gatcgttcgg tacgctgttt ttgttcacgg
    54121 tgatgttggc acggcccaga gcggcgtctg cttctttacc ggtcaggttt ttatcaacca
    54181 gatcaaccag gaacaggtgg ttatcagtac cgccggaaac cactttgtag ccgcgctcga
    54241 ggaacacttc taccatcgct ttagcgtttt tagcgacctg ctgctggtaa gttttgaact
    54301 caggctccat cgcttctttc agagcaaccg ctttaccggc gattacgtgc atcaacggac
    54361 cgccctgacc accagggaaa acagcagagt tcagtttttt gtacagctct tcactaccac
    54421 ctttcgccag gatcaggcca ccgcgcggac ccgccagggt tttgtgagtg gtggtagtaa
    54481 caacgtgagc atgaggaacc gggttcgggt agacgccagc tgcaaccagg cccgcaacgt
    54541 gcgccatatc aacgaacagg taagcaccga tgctgtcagc gatttcacgc attttcgccc
    54601 agtccaccac gccggaatat gcagagaagc caccgataat cattttcggt ttgtgttctt
    54661 tggcttgttt ttccagatcg gcgtagtcga tatgaccggt agcatcgata ccgtaaggaa
    54721 cgatgttgta cagtttaccg gagaagttaa ccggagaacc gtgagtcagg tgaccgccat
    54781 gcgccaggtt catacccaga acggtatcgc ctggttccag cagcgcggtg tacaccgcaa
    54841 agttagcctg ggagccggag tgcggctgga cgttagcgta gtcagcgccg aacagttctt
    54901 tcgcacgatc gatcgccagt tgttcaacga tatcaacata ctcgcaaccg ccgtagtagc
    54961 gtttgcccgg ataaccttca gcatatttgt tggtcagctg agaaccctgc gcctgcatta
    55021 cgcgcgggct ggtgtagttt tcggaggcga tcagttcgat gtgctcttcc tgacgtactt
    55081 tttcctgctc catagcctgc cacagttcgg catcataatc ggcaatgttc atttcacgct
    55141 ttaacatccg catctcctga ctcagctaac aataaaattt ttggccttta taggcggtcc
    55201 tgttggacaa cggcgaacag tataaccgaa tcattgtgcg ataacaggtc ttgacaaagg
    55261 aatttacgca aacgattacc ttcaggctgc gcaaggcttt ggagaataaa gagcttgcaa
    55321 ccggaaacgg atttcttttc aggtttgtga tgcaaatttt tcacttcatc acattctttc
    55381 tgaaaaacac caaagaacca tttacattgc agggctattt tttataagat gcatttgaga
    55441 tacatcaatt aagatgcaaa aaaaggaaga ccatatgctt gacgctcaaa ccatcgctac
    55501 agtaaaagcc accatccctt tactggtgga aacggggcca aagttaaccg cccatttcta
    55561 cgaccgtatg tttactcata acccagaact caaagaaatt tttaacatga gtaaccagcg
    55621 caatggcgat caacgtgaag ccctgtttaa cgctattgcc gcctacgcca gtaatattga
    55681 aaacctgcct gcgctgctgc cagcggtaga aaaaatcgcg cagaagcaca ccagcttcca
    55741 gatcaaaccg gaacagtaca acattgtcgg tgaacacctg ttggcgacgc tggacgaaat
    55801 gttcagcccg ggccaggaag tgctggacgc atggggtaaa gcctatggcg tactggctaa
    55861 tgtatttatc aatcgcgagg cggaaatcta taacgaaaac gccagcaaag ccggtggctg
    55921 ggaaggtact cgcgatttcc gcattgtggc taaaacaccg cgcagcgcgc ttatcaccag
    55981 cttcgaactg gagccggtcg acggtggcgc agtggcagaa taccgtccgg ggcaatatct
    56041 cggcgtctgg ctgaagccgg aaggtttccc gcatcaggaa attcgtcagt actctttgac
    56101 tcgcaaaccg gatggcaaag gctatcgtat tgcggtgaaa cgcgaagagg gtgggcaggt
    56161 atccaactgg ctgcacaatc acgccaatgt tggcgatgtc gtgaaactgg tcgctccagc
    56221 aggtgatttc tttatggctg tcgcagatga cacaccagtg acgttaatct ctgccggtgt
    56281 cggtcaaacg ccaatgctgg caatgctcga cacgctggca aaagctggcc acacagcaca
    56341 agtgaactgg ttccatgcgg cagaaaatgg cgatgttcac gcctttgccg atgaagttaa
    56401 ggaactgggg caagcactgc cgcgctttac cgcgcacacc tggtatcgtc agccgagcga
    56461 agccgatcgc gctaaaggtc agtttgatag cgaaggtctg atggatttgg gcaaactgga
    56521 aggtgcgttc agcgatccga caatgcagtt ctatctctgc ggcccggttg gcttcatgca
    56581 gtttgccgcg aaacagttag tggatctggg cgtgaagcag gaaaacattc attacgaatg
    56641 ctttggtccg cataaggtgc tgtgatttga tgttgccgga tggaaacatc cggcaaccct
    56701 tgacgcggtt taaattgccg cgtcgtcctc ttcaccggta cggatgcgaa tgacccgtgc
    56761 cacgtcgaag acgaagattt taccgtcacc gattttgccg gtttgcgccg tgcgaataat
    56821 ggtatcaaca caggtatcga caatgtcgtc cggtacgaca atctcaattt tcactttcgg
    56881 cagaaaatcc accatatact ccgcgccgcg gtacagctcg gtatggcctt tctggcggcc
    56941 aaaacctttc acctcagtga ccgtcatgcc ggtaatgccg acttcagcca gtgcttcgcg
    57001 aacatcgtcc agcttgaagg gtttaataat cgcatcaatc tttttcatgc tattccttga
    57061 aaaggtcgcc tgtcttttga tctgctaaac gtaacacata acgccaattc attccttgaa
    57121 atcgtttgca tccagctcgt gtcgggaaag cagtttataa aattctgtcc ggttgcgccc
    57181 cgccattctc gccgcgtggg tgacgttgcc tttggtaatt tgcagcagct tacgcaaata
    57241 gttgagttca aactgattac gtgcctcaac aaaggttggc agcgccgtgt tttcgccctc
    57301 cagcgcctgc tctactaatg catcgctaat caccggagat gaggtcagcg ccacgcactg
    57361 ttcaatcacg ttgaccaact ggcgtacatt acccggccag ctagcggtca tcaggcgttt
    57421 catcgcatcg gtggagaacg cgcggacaaa cggtttatgt cgctctgccg cctggcgcaa
    57481 caggtgattt gccagtagcg gaatgtcttc tgtgcgctct gccagagccg gaattttcag
    57541 gctgacaacg ttgaggcggt aatagagatc ttcacggaat tccccgcgcg ccatcgcttt
    57601 tggcaggtcg cggtgagtgg cggaaataat ccgcacgtcg atatcaatat cgcggttact
    57661 gcccagcggg cgcactttac gctcctgcaa cacgcgcagc agtttaacct gcaacggtgc
    57721 gggcatatcg ccaatctcat cgagaaatag cgtaccgcct tccgccgcct ggaataaacc
    57781 ttcgcgattg ctgacagcgc cagtaaacgc gccacgcgca tgaccaaaca gctccgactc
    57841 cagcaattgc tcgggcaatg caccacagtt aatagcaata aatggtttgc tattgcgcgg
    57901 gctagcgttg tggatagcct gggcgaaaat ctctttcccg gtgccgctct gaccgttaat
    57961 caaaacgcta acgtctgatt gcgccaccag ccgcgcctgt tccagcaaac gcagcatcag
    58021 cgggctgcgg gtgacaatcg cctcgcgcca gcgttcatcg gtggctggcg cggattgttc
    58081 cagcgcatcg tcaattgcct gatatagcgc gtctttatcg acaggcttgg tgaggaaact
    58141 gaacactccc tgctgtgttg cagcaacggc atcgggaata gaaccatgtg cggtaagaat
    58201 aattaccggc attcccggct gcactttctg gatttcagca aacagctgca taccatccat
    58261 ttcatccatc cgcaggtcgc tgataactaa atctactttt tcgcgattca gtacccgtaa
    58321 tccttcagca ccactttccg ccgtgaccac gctgtagcct tcgctggtca ggcgcaggcc
    58381 aagcagtttc agcaatcccg gatcgtcatc gaccaataat aaatgcgcag gtttatggct
    58441 catcaggagt gacctcatgg gtggatggcg cgggcttttc actctcgtgc ggcgtatccg
    58501 gcgagaaatt tccagccggt ttgcgggtcg agagctggcg ttcaatatca gtcaggtttt
    58561 ccagcttgcg ggtggtgagt tccagttgct gctgtagaac gtgatgttgc tggcgcaaag
    58621 tatccagctc gctgtcgctg gactgctgga gtttgctgta gcgttggcgc tcttccgcca
    58681 gttgcagttg cagcgcctga ccgtcgcgcc aaagttgata cagagggcga acctgtgccg
    58741 ggatttcggt acttagcgca tcaatgcgcg cgaccagctg gcggcgctca tacggcgtaa
    58801 ttttggcatc ggcgagcaaa atcccttgtt taaaggtatt ttgccagctg ccgtcgttat
    58861 attggcgagc ttgctgacgc gactgcgcag gcattaaacg atcagcacaa tccatcgccc
    58921 gcagccagta aagcggattg gtttcggttg atttaccttg cagcgcccag atgtcgctac
    58981 attcagtaga aagatagtca gccagttgat aaaccggaat tttttcttct gctggcgtat
    59041 taatggctgg cttattgtga ctctgcacgc aacccagcaa tgccagacat ggcagccctg
    59101 ccagccacag ccgtcggggc aataatcgtt gaaaaatgtg tcgcatattc accagactta
    59161 atgcctatcc cagtgggcgt aattgttgca ggcagtttgg acatggacag cgcggagaaa
    59221 ccggagcgta cacgtcgtac gtgaggattt cgagcactgc ccggggccga actggcaaat
    59281 aaaatagcct gatgggatag gctgttagca ttatttcgtg tttttcgacg acggcaattc
    59341 aatgcggaaa caaacgtctt gcccgctctc gtcgaccaga tacagttccc cttgcatacg
    59401 gcgaatacaa tccctggcaa tgcttaatcc cagaccgctg cccttcaccg ccccttttcg
    59461 ctggtggctt ccttgaaaaa agggttcgaa gatcatggcg cgttcctctt gcggaatggg
    59521 tgtgcctgta ttgatgacat caatataaac ccgcgcacca tgtaagctgc tgcgaaggca
    59581 aatgttaccg gattcagccc cgtagtgcac cgcattggag taaagattat ccagtacgct
    59641 catcagcagc attggctccg ccaggcaagc tgttgctttg agatcgacgt cggtatgcat
    59701 cattttagcc cgtgcgggca ggctatgagc agaaaccacc atttccacca gtggcgctaa
    59761 ctcaacattc tccagttcca ccgcaccgtc cgcctgttta cggttgtaat caagcagttg
    59821 ttcgatcaac ttttgcaaat tgcggctgct gctatcaaga atgctcacca cctctttttg
    59881 ctctggcgta agcggcccaa caacctggtc agccagtaat tcagtgccct cacgcatact
    59941 cgccagcggc gtttttaatt catgagataa atgtcttaag aattgatgac gttgggattc
    60001 cagccatgac aggcgctcac ttaaccaaag aatacgttgc ccaaccgagc gtaactcact
    60061 cggtccactg aacgaaacgc tattgcccag agaacgccct tctcccaacc ggttgatcat
    60121 cctctcgata ttttttaccg gaccgataat catccgcgtg aaaagcagta ccatcaccag
    60181 actcaccaga aatagcacca gcgattgcca accaaaatat tgcccacgtt cggcgatttc
    60241 acgctgaagt tgctgcccac gagagaacac cactgtgcgc gtggcctgca ccatttcggt
    60301 attggcactg gcaaaggctt ccagacgcgc ggcggcggcg gcatcgggac cgctgttgtt
    60361 acactgaagt tgagccagat tgttcaagtc ctgacgcaat gcctggtaaa gtttatcgtc
    60421 tggcagtacg cctgcgtggg catcgagcat ttcgctataa cgcttgcgct ggctttgata
    60481 aaccttcgca agcgtagggt cgtccagcac gcaatactga cggtagctgc gctccatttc
    60541 cagtgccgcg ttggtcatcg cttcactgcg ccgggcatca acaagcgtag tgcggttaac
    60601 cagcgccgcc tgatcgctaa gcgcattcag gctttgccag gcttgccatg ccagcaccaa
    60661 caggggtagc agaatcagca aaaatgccag cattaccagt tgtcgtaatg agcggggaaa
    60721 aacgggccag cgtttcaagg tgttactctc gtcagacgcg aatagcctga tgctaaccga
    60781 ggggaagttc agatacaaca aagccgggaa ttacccggct ttgttatgga ataaggcggt
    60841 gcctaactcg acgtttcgcc cgatggttga tatagctacg ctgatatcag aagttggacg
    60901 gcaggcacct tgttgtgcgt cattcgaatt ttatgtagca cgtcccgaag gggctgacat
    60961 aagtcggtga atgagccact ggttactatt atgcagtaac tgtgccaata aagaaaatag
    61021 tttggtaacg tactgattat acgctgcttt gagggattta ttgcttcttc gctatttgaa
    61081 tgtaaatcag ccactgtgtc gctaaaaaga gacaacttaa gataaactta ttagataata
    61141 ttaaaatcaa taagttaagt gtcgccagaa agcgacacgg cacaccaccc attgtcgtga
    61201 tttacagaca caaaaaagct cccgaagttg ggagcttata atagcggttg gtgcttaatg
    61261 ccggatgcgg cgtgaatgcc tcatccgggc tgcaaaacca atgggctgac gacttacccc
    61321 aactgcttac gcgcattgcg gaaaatgcgc atccatgggc catcctcgcc ccagttttcc
    61381 ggatgccagg agttgctgac agtacggaaa acacgttccg ggtgcggcat cataatggtg
    61441 actcgaccac tttcagtcgt gactgccgta ataccgttcg gtgaaccgtt cgggttagcc
    61501 gggtaggttt cagtgacttt accgaagtta tcgacatagc gcagtgccac cagccctttg
    61561 ctttccagcg ccgccagatg cgccgcatca cgcacttcca cgcgcccttc accatgagag
    61621 acagcaatcg gcatttgcga gcccaccatc ccctgcaaca gcagagacgg gctttgggtt
    61681 acttcaacca ggctgaaacg cgcttcaaag cgatcggagg tattgcgcac aaaacgtggc
    61741 cacaactcac tacctgggat cagttcgcgc aggttagaca tcatctggca accgttacat
    61801 acccccagcg ccagcgtttg cggacgatgg aagaaggttg caaactcatc gcgtacacgg
    61861 tcattgaaca ggatagactt cgcccaacct tcaccggcac ccagcacatc accgtaggag
    61921 aaaccgccgc acgcgaccag ggcgtggaaa tcctccaggc ccgtgcgtcc agccagcaga
    61981 tcgctcatat gcacgtcgat agcatcgaag cctgcacggt ggaaagctgc cgccatttca
    62041 acatgcgagt tcacaccctg ctcacgcagc acggcaactt tcgggcgcgc gccagtggca
    62101 ataaacggtg ctgccacatc ttcgttgata tcaaacgaca gtttcacatt caggcccgga
    62161 tcggcgtcgt tggatttcgc ctggtgctcc tggtcagcac actccgggtt gtcacgcagg
    62221 cgctgcatct gccaggtagt ttctgcccac cagacacgca acgtggtgcg gctttcgcta
    62281 aatacagtct gcccgttggc agtaatcaca aaatggtcac cggaaaccgc ttgcccaaca
    62341 taatggacgc aatcagcaag cccatgctgt gccagtaccg cttcaactga ttcacggtca
    62401 gcggctcgaa cctgaatcac cgcacccagt tcttcgctaa acaatgcagc cagacgatcg
    62461 tcacccagag tggcgatatc tgcgtcaatg ccacaatgac cagcaaaggc catttccgcc
    62521 agcgttacca gcaggccgcc atcagagcgg tcgtggtacg cgagcagctt acgctgtgct
    62581 accagcgcct gaaccgcgtc atagaaacct ttcagttgcg cgacgtcgcg tacatctgcc
    62641 ggtttgtcgc caagctgacg ataaacctgc gccagcgccg ttgcgcccag cgcgttattg
    62701 cctttgccca gatcaatcag cagtaatgcg ttatcttcgg tagaaagctg cggcgtgatg
    62761 gtgtgacgca catcttccac acgggcaaat gcagaaatca ccagcgacag cggcgacgtc
    62821 atttcgcgct cttcgttacc ttcctgccag cgggttttca tcgacataga gtctttaccc
    62881 accgggatcg tcaggcccag cgccggacaa agttcttcgc ccacagcttt aacggcttca
    62941 tacaggcccg catcttcacc agggtggcct gccgccgcca tccagttggc ggaaagttta
    63001 atgcgtttga tatcgccaat ctgtgttgcg gcaatattgg ttaacgcttc accgaccgcc
    63061 agacgggcag aggcggcgaa atccagcagc gcaaccggcg cacgctcgcc aatcgccatc
    63121 gcttcgccgt agtagctgtc gaggctggcg gtagtgaccg cgcagttagc gaccggcacc
    63181 tgccacggac ccaccatctg atcgcgcgct accatgccgg ttacgctgcg gtcaccaatg
    63241 gtcaccagga aagttttctc cgccacggtc ggcaggtgca gcacgcgttt caccgcgtca
    63301 gcaatggtaa tcccttcacg aaccagcgca tcacccttcg ctttcagcgt ttgtacttcg
    63361 cgggtcatct tcggcgtttt accaagcagt acgtccagcg gcagatcgat cggctgatta
    63421 tcaaaatgac gatcgtgcaa agaaagatgc agttcttcgg tcgcttcacc aatcaccgcg
    63481 tatggcgcgc gctcacgctt acacagttcg tcaaacagcg gtaactgatc ggcagcaacc
    63541 gccagcacgt agcgttcctg ggattcgtta caccagattt ccagcgggct catgcccggt
    63601 tcgtcgctca ggatatcgcg cagttcgaat ttaccgccgc gcccgccgtc gctcaccagt
    63661 tccggcatgg cgttagaaag accgcctgcg ccaacgtcgt ggataaacag gattgggttg
    63721 gcatcaccaa gctgccagca acggtcgatc acttcctggc agcgacgctc catctccggg
    63781 ttgtcgcgct gtacggaagc gaagtcgagg tcggcatcag actgaccaga cgccatggaa
    63841 gacgccgcgc cgccgccaag accgatgttc attgccgggc cgccgagaac gaccagcttc
    63901 gcaccaacgt tgatctcgcc tttttgtacg tgatcggcac gaatgttgcc gatcccgccc
    63961 gccagcatga tcggtttgtg ataaccgcgc agctcttcgc cattgtggct gttcactttt
    64021 tcttcataag tacggaagta gccgttcagt gccggacgac caaattcgtt gttaaatgcc
    64081 gcgccaccca gcgggccttc ggtcatgatg tccagcgcgg tgacaatgcg ctcaggctta
    64141 ccgaaatctt cttcccacgg ctgttcgaag ccaggaattc gcaggttgga aacggagaaa
    64201 ccaaccaaac cagctttcgg cttcgcgccg cgtccggtgg caccttcatc gcggatttca
    64261 ccgccggaac cggtcgccgc gcccggccac ggagaaatcg ccgtcgggtg gttgtgggtt
    64321 tcaactttca tcagaatatg cgccggttcc tgatggaaat cgtagcggcc cgtttcgtgg
    64381 tcagcaaagt agcggcccac ttcagcccct tccatgacgg cggcgttatc tttataagca
    64441 gagagaacgt gatccggcgt agtttcgaag gtgtttttga tcatcttaaa cagcgatttc
    64501 ggctgctgtt caccatcgat aacccagtcg gcgttaaaaa ttttgtggcg gcagtgctcg
    64561 gagttcgcct gggcaaacat atacagttcg atgtcgttcg ggttacgacc aagctttgtg
    64621 aaagcatcct gcaaatagtc aatttcatct tccgccagtg ccaggccaag acgcaggtta
    64681 gcgtcgatta gcgcctgacg gcccaacccc agcaaatcaa cgctggtgac cggagtcggt
    64741 tgatggtgtg caaacagctg ctctgcatca tctaaagcaa aaaagaccgt ttccatcatg
    64801 cggtcgtgca gttcagcggt aacctgctgc cattgttcat tggtcagcgt accggcttct
    64861 atatagtaag ccacgccgcg ctcaagacgg tttacctgtt gcagcccgca gttatgggca
    64921 atatcggtcg ctttcgaaga ccagggagag atggtgccag gacgcggggt caccagcagg
    64981 agtttgcctt gcggggcgtg gctggcgagt gccgggccat atttcagcag gcgttcaagt
    65041 tgtgcgtgct catcatcgtt taacggcgca ttgaggtcag caaaatggac atactcggcg
    65101 taaatagtgt gaaccgggag cctggcagcc tgaaaacgtg ccagcagttt gttgattcgg
    65161 aatgccgaca atgcaggcga accacgcaga atttccatca taagtctctc gtcttctaag
    65221 ctttcggtgt acccaagggg ggaaacgggc gtcattataa agaatctgat gcgctgacga
    65281 aaccgtttgc gtggaaataa aatcaccatc gcgaattagc aacgcgtgcc gccaatggct
    65341 gtaataagtt gccaactggc gcaggtttac gcaaaatgcc gctcatttat gagtaaacct
    65401 ttcactatta ttacgttttt tcaagctggg acgcgcacga cacagagaat taa
//
LOCUS       DACBVI010000008        65432 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.008, whole genome shotgun sequence.
ACCESSION   DACBVI010000008 DACBVI010000000
VERSION     DACBVI010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.008"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..414)
                     /gene="folK"
                     /locus_tag="IU325_02630"
     CDS             complement(<1..414)
                     /gene="folK"
                     /locus_tag="IU325_02630"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HAP5807934.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVF"
     gene            complement(411..1829)
                     /gene="pcnB"
                     /locus_tag="IU325_02635"
     CDS             complement(411..1829)
                     /gene="pcnB"
                     /locus_tag="IU325_02635"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HAP5807935.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRSRRPRKRAPRREGTA"
     gene            complement(1868..2794)
                     /gene="gluQRS"
                     /locus_tag="IU325_02640"
     CDS             complement(1868..2794)
                     /gene="gluQRS"
                     /locus_tag="IU325_02640"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HAP5807936.1"
                     /translation="MLPPYFLFNEMTDTHYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARHGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRELHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWRLTAVPESAIVNSTFSNASC"
     gene            complement(2831..3286)
                     /locus_tag="IU325_02645"
     CDS             complement(2831..3286)
                     /locus_tag="IU325_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding transcription factor
                     DksA"
                     /protein_id="HAP5807937.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3464..4168)
                     /gene="sfsA"
                     /locus_tag="IU325_02650"
     CDS             complement(3464..4168)
                     /gene="sfsA"
                     /locus_tag="IU325_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP5807938.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAIICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     LSAEGMALKKSLPVTL"
     gene            complement(4183..4713)
                     /gene="thpR"
                     /locus_tag="IU325_02655"
     CDS             complement(4183..4713)
                     /gene="thpR"
                     /locus_tag="IU325_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HAP5807939.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPDAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4787..7216
                     /gene="hrpB"
                     /locus_tag="IU325_02660"
     CDS             4787..7216
                     /gene="hrpB"
                     /locus_tag="IU325_02660"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HAP5807940.1"
                     /translation="MSSLPVAAVLPELLTALDCASQVLLSAPTGAGKSTWLPLQLLAH
                     SAIHGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVIISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LRMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAYADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMSGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7310..9844
                     /gene="mrcB"
                     /locus_tag="IU325_02665"
     CDS             7310..9844
                     /gene="mrcB"
                     /locus_tag="IU325_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HAP5807941.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIANMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10064..12253
                     /gene="fhuA"
                     /locus_tag="IU325_02670"
     CDS             10064..12253
                     /gene="fhuA"
                     /locus_tag="IU325_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HAP5807942.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIASDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVGHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRVSYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12304..13101
                     /gene="fhuC"
                     /locus_tag="IU325_02675"
     CDS             12304..13101
                     /gene="fhuC"
                     /locus_tag="IU325_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HAP5807943.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13101..13991
                     /gene="fhuD"
                     /locus_tag="IU325_02680"
     CDS             13101..13991
                     /gene="fhuD"
                     /locus_tag="IU325_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HAP5807944.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRILDNAIGGKA"
     gene            13988..15970
                     /gene="fhuB"
                     /locus_tag="IU325_02685"
     CDS             13988..15970
                     /gene="fhuB"
                     /locus_tag="IU325_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HAP5807945.1"
                     /translation="MSKRIALFPALLLALLVIVAAALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16005..17285)
                     /gene="hemL"
                     /locus_tag="IU325_02690"
     CDS             complement(16005..17285)
                     /gene="hemL"
                     /locus_tag="IU325_02690"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HAP5807946.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17510..18931
                     /gene="clcA"
                     /locus_tag="IU325_02695"
     CDS             17510..18931
                     /gene="clcA"
                     /locus_tag="IU325_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HAP5807947.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGVIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            18955..19020
                     /locus_tag="IU325_02700"
     CDS             18955..19020
                     /locus_tag="IU325_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5807948.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19013..19357
                     /gene="erpA"
                     /locus_tag="IU325_02705"
     CDS             19013..19357
                     /gene="erpA"
                     /locus_tag="IU325_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HAP5807949.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19404..20027)
                     /locus_tag="IU325_02710"
     CDS             complement(19404..20027)
                     /locus_tag="IU325_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5807950.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20065..20865)
                     /gene="btuF"
                     /locus_tag="IU325_02715"
     CDS             complement(20065..20865)
                     /gene="btuF"
                     /locus_tag="IU325_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HAP5807951.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARAPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(20858..21556)
                     /gene="mtnN"
                     /locus_tag="IU325_02720"
     CDS             complement(20858..21556)
                     /gene="mtnN"
                     /locus_tag="IU325_02720"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP5807952.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21640..23157
                     /locus_tag="IU325_02725"
     CDS             21640..23157
                     /locus_tag="IU325_02725"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HAP5807953.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFNKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23287..24711
                     /gene="degP"
                     /locus_tag="IU325_02730"
     CDS             23287..24711
                     /gene="degP"
                     /locus_tag="IU325_02730"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HAP5807954.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            24866..26023
                     /locus_tag="IU325_02735"
     CDS             24866..26023
                     /locus_tag="IU325_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family transcriptional regulator"
                     /protein_id="HAP5807955.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26077..26463)
                     /locus_tag="IU325_02740"
     CDS             complement(26077..26463)
                     /locus_tag="IU325_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HAP5807956.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26776..27600)
                     /gene="dapD"
                     /locus_tag="IU325_02745"
     CDS             complement(26776..27600)
                     /gene="dapD"
                     /locus_tag="IU325_02745"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HAP5807957.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFANYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27631..30303)
                     /gene="glnD"
                     /locus_tag="IU325_02750"
     CDS             complement(27631..30303)
                     /gene="glnD"
                     /locus_tag="IU325_02750"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HAP5807958.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEELIEARTEFIDQLLQRLWIDAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30365..31159)
                     /gene="map"
                     /locus_tag="IU325_02755"
     CDS             complement(30365..31159)
                     /gene="map"
                     /locus_tag="IU325_02755"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP5807959.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSPETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31527..32252
                     /gene="rpsB"
                     /locus_tag="IU325_02760"
     CDS             31527..32252
                     /gene="rpsB"
                     /locus_tag="IU325_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP5807960.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32510..33361
                     /gene="tsf"
                     /locus_tag="IU325_02765"
     CDS             32510..33361
                     /gene="tsf"
                     /locus_tag="IU325_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP5807961.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33508..34233
                     /gene="pyrH"
                     /locus_tag="IU325_02770"
     CDS             33508..34233
                     /gene="pyrH"
                     /locus_tag="IU325_02770"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP5807962.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34525..35082
                     /locus_tag="IU325_02775"
     CDS             34525..35082
                     /locus_tag="IU325_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP5807963.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35174..36370
                     /gene="ispC"
                     /locus_tag="IU325_02780"
     CDS             35174..36370
                     /gene="ispC"
                     /locus_tag="IU325_02780"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HAP5807964.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36559..37317
                     /gene="ispU"
                     /locus_tag="IU325_02785"
                     /gene_synonym="uppS"
     CDS             36559..37317
                     /gene="ispU"
                     /locus_tag="IU325_02785"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HAP5807965.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37330..38187
                     /gene="cdsA"
                     /locus_tag="IU325_02790"
     CDS             37330..38187
                     /gene="cdsA"
                     /locus_tag="IU325_02790"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP5807966.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWSVALLL
                     VLFYPGSAAIWRNSKTLRIIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38199..39551
                     /gene="rseP"
                     /locus_tag="IU325_02795"
     CDS             38199..39551
                     /gene="rseP"
                     /locus_tag="IU325_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HAP5807967.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWTFEPDKEDPITSLGIRPR
                     GPQIESVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39581..42013
                     /gene="bamA"
                     /locus_tag="IU325_02800"
     CDS             39581..42013
                     /gene="bamA"
                     /locus_tag="IU325_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HAP5807968.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42135..42620
                     /gene="skp"
                     /locus_tag="IU325_02805"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42135..42620
                     /gene="skp"
                     /locus_tag="IU325_02805"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HAP5807969.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42624..43649
                     /gene="lpxD"
                     /locus_tag="IU325_02810"
     CDS             42624..43649
                     /gene="lpxD"
                     /locus_tag="IU325_02810"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HAP5807970.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSRIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43754..44209
                     /gene="fabZ"
                     /locus_tag="IU325_02815"
     CDS             43754..44209
                     /gene="fabZ"
                     /locus_tag="IU325_02815"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP5807971.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44213..45001
                     /gene="lpxA"
                     /locus_tag="IU325_02820"
     CDS             44213..45001
                     /gene="lpxA"
                     /locus_tag="IU325_02820"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HAP5807972.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45001..46149
                     /gene="lpxB"
                     /locus_tag="IU325_02825"
     CDS             45001..46149
                     /gene="lpxB"
                     /locus_tag="IU325_02825"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HAP5807973.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHMLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46146..46742
                     /gene="rnhB"
                     /locus_tag="IU325_02830"
     CDS             46146..46742
                     /gene="rnhB"
                     /locus_tag="IU325_02830"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP5807974.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPSRPIAGLN
                     DSKKLSEKRRLALCEEIKDKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPSMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRCSFGPVKRALGLAS"
     gene            46779..50261
                     /gene="dnaE"
                     /locus_tag="IU325_02835"
     CDS             46779..50261
                     /gene="dnaE"
                     /locus_tag="IU325_02835"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP5807975.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVMRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50274..51233
                     /gene="accA"
                     /locus_tag="IU325_02840"
     CDS             50274..51233
                     /gene="accA"
                     /locus_tag="IU325_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP5807976.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLSVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKVQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51331..53472
                     /gene="ldcC"
                     /locus_tag="IU325_02845"
     CDS             51331..53472
                     /gene="ldcC"
                     /locus_tag="IU325_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HAP5807977.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53529..53918
                     /locus_tag="IU325_02850"
     CDS             53529..53918
                     /locus_tag="IU325_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP5807978.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            53983..55281
                     /gene="tilS"
                     /locus_tag="IU325_02855"
     CDS             53983..55281
                     /gene="tilS"
                     /locus_tag="IU325_02855"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP5807979.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRAENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELVQWARQYDLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDIAHCQKPQGTLQIAPMLAMSDARRAAIIRRWLAGKNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVAGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIIGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGEKGVSFVWQKTLS"
     gene            complement(55330..55590)
                     /gene="rof"
                     /locus_tag="IU325_02860"
     CDS             complement(55330..55590)
                     /gene="rof"
                     /locus_tag="IU325_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HAP5807980.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGATRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55577..55777)
                     /locus_tag="IU325_02865"
     CDS             complement(55577..55777)
                     /locus_tag="IU325_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5807981.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            55943..56488
                     /locus_tag="IU325_02870"
     CDS             55943..56488
                     /locus_tag="IU325_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5807982.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSIWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTVWQQPS"
     gene            56485..56907
                     /locus_tag="IU325_02875"
     CDS             56485..56907
                     /locus_tag="IU325_02875"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP5807983.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRNQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            56921..57631
                     /gene="nlpE"
                     /locus_tag="IU325_02880"
                     /gene_synonym="cutF"
     CDS             56921..57631
                     /gene="nlpE"
                     /locus_tag="IU325_02880"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HAP5807984.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLTARDHSEKPMLLSLEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(57662..58486)
                     /locus_tag="IU325_02885"
     CDS             complement(57662..58486)
                     /locus_tag="IU325_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HAP5807985.1"
                     /translation="MDKPKVHCRLLLPCFLFLSACTVDISQPDSSATAVDAGAKTWAV
                     KFQHQSSFTEQSIKEITEPDLKPGDLLFSSSHGVTSFGIRVFSTSSVSHVAIYLGENN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(58539..60257)
                     /gene="proS"
                     /locus_tag="IU325_02890"
     CDS             complement(58539..60257)
                     /gene="proS"
                     /locus_tag="IU325_02890"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP5807986.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60368..61075)
                     /gene="tsaA"
                     /locus_tag="IU325_02895"
     CDS             complement(60368..61075)
                     /gene="tsaA"
                     /locus_tag="IU325_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HAP5807987.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPNASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61072..61476)
                     /gene="rcsF"
                     /locus_tag="IU325_02900"
     CDS             complement(61072..61476)
                     /gene="rcsF"
                     /locus_tag="IU325_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HAP5807988.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(61594..62409)
                     /gene="metQ"
                     /locus_tag="IU325_02905"
     CDS             complement(61594..62409)
                     /gene="metQ"
                     /locus_tag="IU325_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HAP5807989.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62449..63102)
                     /gene="metI"
                     /locus_tag="IU325_02910"
     CDS             complement(62449..63102)
                     /gene="metI"
                     /locus_tag="IU325_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HAP5807990.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63095..64126)
                     /gene="metN"
                     /locus_tag="IU325_02915"
     CDS             complement(63095..64126)
                     /gene="metN"
                     /locus_tag="IU325_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HAP5807991.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64313..64885
                     /gene="gmhB"
                     /locus_tag="IU325_02920"
     CDS             64313..64885
                     /gene="gmhB"
                     /locus_tag="IU325_02920"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAP5807992.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     VTPEAENAADWVLNSLADLPQAIKKQQKPA"
     gene            65251..>65432
                     /locus_tag="IU325_02925"
     rRNA            65251..>65432
                     /locus_tag="IU325_02925"
                     /product="16S ribosomal RNA"
BASE COUNT        15510 a        16200 c        18016 g        15706 t
ORIGIN      
        1 aaacaccagc tccggcgcaa tttcaaacag cggccacagc ataaatccac gattcttcat
       61 atcgtagtgc ggaacggtca ggcgttcggt atttattact tcattaccaa atagcatgat
      121 gtcgagatcc agtgtgcgcg gcccccagcg ttccgcttta cggacgcgac cttgttgtaa
      181 ctcaatacgt tgtgtgtggt tgagtaactc ttcaggtgca agagaggttt ccagcgccac
      241 ggctgcgttt aagtaatcgg gttgatcttg cggccccagc ggtggggtgc ggtaaaacga
      301 agaaacggca agaatgtggc tttcagggat atcgcctaat gctttcaggg cagcattgac
      361 ctgctccagc ggagaggcca gattgctgcc tatggcaata tacgccactg tcatgcggta
      421 ccctcacgac gcggtgcgcg tttgcgtgga cgacgagaac ggcgacgcgg tgacggctct
      481 tcatccagct cgttgagcat ccctttttga tctggtggcg cggaaacctg gaactccccc
      541 caccatttca caagacgctg cagttcagcg ttacgttcaa cttcagctcg caaggccaac
      601 aggtcataag ccgcacggaa cttaggatgc tccagcagtt tccaggcgcg tttaccctga
      661 cgtcgggaca tacgcaactg caactgccag atatcgcggg ttaacgtcgt cagacgtttc
      721 gggatcgcca gtgaacggca ggcttcgtcc agcacgtcgt tcatcgccag cgcgaaagcg
      781 tcgtgatagg tcaggccgct ttcctgggcg atcttctgtg ccgtctccag cagtgggtac
      841 cagaacatgg cggcaaacag gaacgccggg ttcacgcgca tatcgttatg gatacgcgta
      901 tcggtattct tcagcacctg ttcaatgatc cgctccatcg ggctgtcgcc attttccgtg
      961 aagtagcggg taatggtcgg gaacagcggc tggaacaggt gatattcaca cagcagctta
     1021 taggtttcgt aaccgtagcc cgcttgcagc agtttcaaag attcttcaaa cagacgtgcc
     1081 ggtggaatat cgttcagcag ggtagcgagg cgagggatcg gttctgcggt ttcaggactg
     1141 atacgcatac ccaatttggc ggcaaaacgt accgcgcgca gcatccgtac cggatcttca
     1201 cggtagcgcg tttccgggtt accaatcaga cggataacgc cgtctttcag atccttcatg
     1261 ccgccaacgt aatcacggac ggtaaaatcc gctacgctgt aatacaggct gttgatagtg
     1321 aaatcgcggc gctgggcgtc ttcttcgatg gagccgaaaa tgttgtcgcg cagcaacata
     1381 ccgttttgcc cgcgttggga ggtcgtgcgg tcgctgacgt taccttcgtg atgtccacgg
     1441 aaggtcgcaa cttcgataat ctccgggcca aacatcacat gagccagacg gaaacggcga
     1501 cccaccaggc ggcagttacg gaacagttta cgtacctgtt caggcgtggc gttagtggtt
     1561 acgtcaaaat ctttcggctt tttgccaagt aacaggtcgc gcacgccgcc gccaaccagc
     1621 caggcttcgt atcccgcttt attgagcctg tacattacct tcagggcatt ttcactgata
     1681 tctttgcggg aaatagcatg ctgctcacgc gggatcaccg tcacctgtgg acgggcgact
     1741 gcctgttcag cctcgctttc ctcgcggctt agcaccttgc ggcaaaaatt agcgactcgg
     1801 gtaaaaatag tacacctcgg tagtgtcaaa catcattcag gacaaaaaaa tagcggctaa
     1861 tcatagctca gcatgacgca tttgagaatg ttgaatttac aattgccgac tcaggcactg
     1921 cggtgagccg ccagtttttg acggctgact gaaggatttg ctcgacgctg aaatcctgcc
     1981 agtgtgcttc tgcctgttga cccagaaatt gaagtgccgc gattaaaacc gggcgtggat
     2041 cgcctttcgg caacgcaggc gcatgattct gcttggaaag tttagcgcct tgtggattaa
     2101 gcgccagcgg taaatggatg taatctggca ctttccagcc aaaaagctgg tacagcgaga
     2161 tttgccgcac tgtcggttcg atcaaatctg ccccgcggac aatttcgctc acgccctgga
     2221 agtgatcatc caccaccacc gccaggttat aggcaaacaa accatcacgg cgatgaataa
     2281 taaaatcttc ccgtgccagt ttttcgtcgg cgtgaataat gcctcgcagc aggtcagtaa
     2341 actgggtgac cggatgctgc tggcggatac gtactgcggc gttgtctggt ccatgatgta
     2401 attcccggca atgaccgtcg taaataccgc caatgctttg aatacgcgca cgcgtgcagg
     2461 tgcagtaata acttaatcct tgctcatgta accaggcgag tgcttcgcga taggcgtggt
     2521 gacgttgcga ttgccagaga acatcgccgt cccagtgcag gccgtaatgt tccagctggc
     2581 gcaggatagt ttctgcggca ccgggaactt cacgaggcgg gtcgatatct tcaatgcgta
     2641 ccagccagcg accgtgccta gcgcgagcct gtaaatagct gccaagcgcg gcaatcagag
     2701 agccaaaatg aagctcgccg gaaggagagg gggcaaagcg gccaatatag tgtgtgtctg
     2761 tcatctcgtt gaacaaaaaa taaggcggga gcatttcccg cctgtggtaa acgtgatgga
     2821 acggctgtaa ttagccagcc atttgttttt cgcgaatttc agccagcgtt ttgcagtcga
     2881 tgcacagatc ggctgtcggg cgcgcttcca gacggcgaat accaatttca acaccgcagg
     2941 attcgcagta gccgaaatct tcgtcttcca cttttttcag cgttttctcg atctttttga
     3001 tcagcttacg ctcgcgatcg cggttacgca gttcgaggct gaactcttct tcctgggctg
     3061 cacggtctac cgggtccggg aagttggctg cttcatcctg catatgtgta acggtgcgat
     3121 cgacttcatc cctgagttga ttacgccatg cttccagaat acgacggaag tgcgccagct
     3181 gggcttcatt catatactct tcgcccggct tctcctgata tggttccacc ccagcgatgg
     3241 cgagaatact cagggacgat gttttacggt tttgcccttc ttgcatgttg cttctcctta
     3301 acacgcacta tcgatcccca tgttcggggg aaaaatgagg ccgctataaa tagcagatgc
     3361 ttttccggat agcaattatc taaacgtaac acttgacaac tgtgtgagga aaagcgtatt
     3421 tgcgcacgcg accagaatgt aaattaacca gttacttact ctactacaat gtaaccggta
     3481 atgatttttt aagagccatg ccttcggcag aaagttccgc tttgtaagcc agaatttcta
     3541 cccccctctg ttgagcttct gacaatagtt gcgcgtattt ctcatcgatg tggcgcgcgg
     3601 gtgaaaaccg tgtaatggct gaatgcagca cggcaaaaaa gataaccgca cgctggcctt
     3661 cagccgctac gctcatcaac tcccgaaggt gtttctgacc tcgttcagtg accgcatcgg
     3721 gaaaatatcc ctgttcgttt tccgctaacg taaccgattt cacttcaata tagcagtctg
     3781 gacgcgaatc cgcctgcaac ataaagtcaa tacggctgcg ttctgcgccg tatttaactt
     3841 cgcttttcag cgagctatag cctgacagtt ctgaaattga ttcattaagg atagcctctt
     3901 tcgtcaacct gttagcccaa agcgtattga cgcaaataat tgcgccgctc tggctttgag
     3961 tcaattccca ggtgtgtggg tatttccgtt tggtgttgtc tgaagtcgaa taccagacgg
     4021 tatcgccagg cgttgcacaa ccggtcatcg cacccgtatt cgggcagtgt agcgttaatt
     4081 cgcgaccatc gggtgtgatc acatcggcta aaaaacgttt gtaacgctga attagcgtcg
     4141 cgcgctgtag agggggagaa aattccatta cgacaatcct tgttattgcg ttagcgccca
     4201 gcgttttagc ggcgtgtagc gtgtgcgtcc acgcgcaaac gaggaggcat aaagggtgaa
     4261 ctccgtcacc gtatacgacc agttaaaacc tggcggcggg attgtcaccg cctcgctggc
     4321 gtcgcgcaat aaggtaatat gtggatgaaa cggacgattg ctttgaaaac aaccgctgcg
     4381 ggcagcctgt gaacggagca tattcgccag ctggattaag ccgcgtggtg gctgacgcat
     4441 ccctaaccac accacacgcg aacgcagcca ttgaccggcg tcatcaagcg tgagtgtgaa
     4501 acccggttga cgaatccgcc cggctaaaag agaaagcgcc ttctctttct cggcgctcac
     4561 ttcgcctaaa aatgccagag tcagatgcaa attatcggcg gcgaccggac gtcccgcgtc
     4621 aggtgggaag tgtgtggcgc gccagtggat aatctgttcg cggatttctg caggtaagtc
     4681 gatagcaaag aacagccgtt gcggttcaga catgtgaggc actcggttat gaattaccgc
     4741 gatgctacaa tgtgccgcga agaatgttaa ccctctggag cgttttgtgt cgtcgttgcc
     4801 cgttgctgcc gtcttacctg aattacttac cgctctcgat tgtgcatcgc aggtgttatt
     4861 aagtgcgccg accggggccg ggaaatcaac ctggctgccg ctgcaactgc tggcgcattc
     4921 agctattcac gggaaaatca ttctgctgga gccgcgtcgt ctggcggcgc gtaacgttgc
     4981 gcaacggctg gcggagctgc ttaacgaaaa gccaggcgat accgttggct accggatgcg
     5041 tgcgcaaaac tgcgtcgggc cgaatacccg cctggaagtg gttaccgaag gcgtgctgac
     5101 gcgcatgatc cagcgtgacc cggaactgag cggtgttgga ttggtgatcc tcgatgagtt
     5161 tcatgagcgc agcttgcagg cggatttggc gttggcgctg ttactcgatg tgcaacaagg
     5221 tctgcgtgat gaccttaaac tgctgattat gtcggctacg ctggacaacg accgcttgca
     5281 gcaaatgctg ccagaagcgc ccgtcatcat ctcagaaggg cgctcgtttc cggttgaacg
     5341 ccgttattta ccgctgcccg cgcatcagcg ttttgacgaa gccgttgcgg tagccaccgc
     5401 cgaaatgctg cgtcaggaaa gtggatcatt actgttattt ttacctggcg tcggagaaat
     5461 tcagcgcgtg caggaacaac tggcttcgcg catcggcagt gatgtgttgc tctgcccgct
     5521 gtatggcgcg ttgtcgctga acgatcagcg aaaagcgatc ctcccggcac cgcaagggat
     5581 gcgcaaagtg gtgctggcga ccaatattgc tgaaaccagt ttaaccattg aaggtattcg
     5641 tctggtggtg gattgtgccc aggagcgtgt ggcgcgtttt gatccgcgca cggggcttac
     5701 gcggttgatt actcaacgcg ttagtcaggc atccatgacg cagcgtgccg ggcgcgccgg
     5761 gcgtctggag ccgggtatct gcctgcattt aatcgccaaa gaacaagcag aacgcgctgc
     5821 ggcgcaaagt gaaccggaaa tattacaaag cgatctttcc ggtttgctga tggaattact
     5881 gcaatgggga tgcagcgatc cggcgcagat gagctggctg gatcaaccgc caacggtgaa
     5941 tctactggcc gcgaaacgcc tgttacggat gctgggggcg ctggacggtg aacggcttag
     6001 tgcgcaaggg caaaaaatgg cagcgctggg taacgatccg cgtttagcgg caatgctggt
     6061 tagcgcgaag aacgacgacg aagctgctac cgcggcaaaa attgccgcca ttctcgaaga
     6121 gccgccacgg atgggcaata gtgacctggg cgtggcgttt tcgcgcaatc aaccagcctg
     6181 gcagcaacgt agtcagcaac tgttaaaacg tttaaacgta cgcggcggtg aggcagacag
     6241 ttctcttatc gcgccgctac ttgccggggc gtatgccgat cgcattgctc gtcgccgtgg
     6301 gcaagatgga cgctatcaac tggcaaacgg catgggggcg atgctcgatg ccgacgatgc
     6361 gctaagccgc cacgaatggt tgatcgcacc gttattattg cagggcagcg cctcgccgga
     6421 tgcgcggatt ttactggcgc tgccggtcga tattgatgag ttagtacaac gctgcccgca
     6481 gctggtacag cagtcggaca ccgtggaatg ggatgacgcg caaggcacgc tgaaagcctg
     6541 gcgtcggctg caaatcggtc agttgacggt gaaagtgcag ccactggcga aaccctcgga
     6601 agacgagttg catcaggcga tgcttaacgg cattcgtgat aaaggtttaa gcgtgctcaa
     6661 ctggacggcg gaagcggaac agctacgctt gcgtttgtta tgcgccgcaa agtggttgcc
     6721 ggaatatgac tggccagcgg ttgatgatga gagtttgttg gcgacgctgg aaacgtggct
     6781 actgccgcat atgagcggcg tgcattcact acgcggcctg aaatcactcg acatttatca
     6841 ggcactacgc ggattacttg attggggaat gcagcaacgt ctggatagtg aattgcctgc
     6901 gcattacact gtgccgacgg gaagccggat cgccattcgt tatcatgaag ataacccgcc
     6961 cgcgctggcg gtgagaatgc aggagatgtt tggcgaggcc accaatccga cgatcgctca
     7021 ggggcgcgtg ccgctggtgc tggagttgct ttcccctgcc caaaggccgc tgcaaatcac
     7081 gcgtgatttg ggggccttct ggaaaggggc gtatcgtgag gtgcaaaagg agatgaaagg
     7141 gcgttatccc aaacacgtct ggccggacga cccggcaaac acggcaccga cgcgacggac
     7201 gaaaaagtat tcgtgaagac caaagcaaat ttgagagata tcttcttctg tcttgcaaca
     7261 gaagaacaga aaatcgggct tttgcgcctg aatattgcgg agaaaaagca tggccgggaa
     7321 tgaccgcgag ccaattggac gcaaagggaa accgacgcgt ccggtcaaac aaaaggtaag
     7381 ccgtcgtcgt tacgaagatg acgatgatta cgacgattat gatgactatg aggatgaaga
     7441 accgatgccg cgcaaaggta agggcaaagg caaagggcgt aagcctcgtg gcaaacgcgg
     7501 ctggctatgg ctgctgctaa aactggctat cgtttttgcc gtgctgatcg ccatttacgg
     7561 cgtttatctc gatcaaaaaa ttcgcagccg tattgatggc aaggtctggc aactgcctgc
     7621 ggcagtttat ggccgaatgg tcaaccttga gccagatatg accatcagca agaacgagat
     7681 ggtgaagctg ctggaggcaa cccagtatcg tcaggtgtcg aaaatgaccc gtcctggcga
     7741 atttaccgtg caggccaaca gcattgagat gatccgccgt ccgtttgatt tcccggacag
     7801 taaagaaggg caggtgcgtg cgcgtctgac ctttgatggc gatcatctgg cgaccatcgt
     7861 caatatggag aacaaccgtc agtttggttt cttccgtctt gatccgcgcc tgatcaccat
     7921 gatctcctcg ccaaacggtg agcaacgtct gtttgtgccg cgcagtggtt tccctgattt
     7981 gctggtggat actttgctgg cgacagaaga ccgccatttc tacgagcatg atgggatcag
     8041 tctctactcc atcggacgtg cggtgctggc aaacctgacc gccggacgca cggtgcaggg
     8101 ggcgagtacg ctgacgcaac agctggtgaa aaacctgttc ctctccagcg agcgctctta
     8161 ctggcgtaaa gcgaacgaag cttacatggc gctgatcatg gacgcgcgtt acagcaaaga
     8221 ccgtattctt gagctgtata tgaacgaggt gtatctcggt cagagcggcg acaacgaaat
     8281 ccgcggcttc ccgctggcga gcttgtatta ctttggtcgc ccagtagaag aattgagcct
     8341 cgaccagcag gcgctgttgg tgggtatggt gaaaggggcg tccatctaca atccgtggcg
     8401 taacccgaaa ctggcgctgg agcgacgtaa tctggtactg cgtctgctgc aacagcaaca
     8461 gattattgat caagaactct atgacatgtt gagtgcacgt ccgctgggcg ttcagccgcg
     8521 cggtggggta atctctccac agccagcctt tatgcagctg gtgcgtcagg agctgcaggc
     8581 aaaactgggc gataaggtaa aagatctctc cggcgtgaag atcttcacta cctttgactc
     8641 ggtggcccag gacgcggcag aaaaagccgc cgtggaaggc attccggcac tgaagaaaca
     8701 gcgtaagttg agtgatcttg aaaccgcgat tgtggttgtt gaccgcttta gcggtgaagt
     8761 tcgtgcgatg gtcggtggtt ctgagccgca gtttgcgggc tacaaccgtg cgatgcaggc
     8821 gcgtcgttcg attggttccc ttgcaaaacc agcgacttat ctgactgcct taagccagcc
     8881 gaaaatctat cgtttgaata cgtggattgc cgatgcgcca atcgcgctgc gtcagccgaa
     8941 tggccaggtc tggtcaccgc agaatgatga ccgtcgttat agcgaaagtg gcagagtgat
     9001 gctggtggat gcgttgaccc gttcgatgaa cgtgccgacg gtgaatctgg ggatggcgct
     9061 ggggctgcct gcggttacgg agacctggat taaactgggc gtaccgaaag atcagctgca
     9121 tccggttccg gcaatgctgc tgggggcgtt gaacttaacg ccaatcgaag tggcgcaggc
     9181 attccagacc atcgccagcg gtggtaaccg tgcaccgctt tctgcgctgc gttcagtcat
     9241 cgcagaagat ggcaaggtgc tgtatcagag cttcccgcag gcagaacgcg ctgttccggc
     9301 gcaggcggca tatctgacac tatggaccat gcagcaggtg gtacaacgtg gtacgggtcg
     9361 tcagcttggg gcgaaatacc cgaacctgca tctggcaggg aaaacaggga ctaccaacaa
     9421 taacgtagat acctggtttg cgggcattga cggcagcacg gtgaccatca cctgggtagg
     9481 ccgtgataac aaccaaccga ccaaactgta tggagccagc ggggcaatgt cgatttatca
     9541 gcgttatctg gctaaccaga cgccaacgcc gctaaatctt gttccgccag aagatattgc
     9601 aaatatgggc gtggactacg acggcaactt tgtttgcagc ggtggtatgc gtgtcttgcc
     9661 ggtctggacc agcgatccgc aatcgctgtg ccagcagagt gagatgcagc agcaaccgtc
     9721 aggcaatccg tttgatcagt cttctcagcc gcagcaacag ccgcaacagc aacctgctca
     9781 gcaagagcag aaagacagcg acggtgtagc cggttggatc aaggatatgt ttggtagtaa
     9841 ttaacgtcta agcgtgacat accggatggc gagttgccat ccggtaaaat aacatcccac
     9901 ctaaagtatt aaccctttct tttcatctgg ttgtttatta acccttcagg aacgctcaga
     9961 ttgcgtaccg cttgcgaacc cgccagcgtt tcgaatatta tcttatcttt ataataatca
    10021 ttctcgttta cgttatcatt cactttacat cagagatata ccaatggcgc gttccaaaac
    10081 tgctcagcca aaacactcac tgcgtaaaat cgcagttgta gtagccacag cggttagcgg
    10141 catgtctgtt tatgcacagg cagcggttga accgaaagaa gacactatca ccgttaccgc
    10201 tgcacctgcg ccgcaagaaa gcgcatgggg gccagctgca actattgcgg cgaagcactc
    10261 tgctactgcg actaaaacgg atacaccaat tgaaaaaacg ccgcagtcta tttcggttgt
    10321 gacaaatgaa gagatgcaga tgcatcaatt tcagtctgta aaagaagcat taggttatac
    10381 acctggcgtt acggttagca gtcgtggtgc ttctaataca tatgattttg ttattattcg
    10441 tggtttctca tctgttggtc tgaatcaaaa taattacctt gatgggctaa aacttcaggg
    10501 caacttttat aacgatgctg tgattgatcc ttacatgctc gagagggttg aactgatgcg
    10561 cgggccgacg tctgttctct atgggaaaag taatcctggt gggattattt ctatggtgag
    10621 taagcgtccg acaactgaac ccctgaaaga aattcagttt aaaatgggga cggataatct
    10681 gtttcagacc ggattcgatt tcagtgatgc gctggatgat aacggtgagt tctcctaccg
    10741 tttgactggc ctcgcacgct caacaaacga acagcagaaa aactctgaat ctcagcgtta
    10801 taccattgct ccatcattct catggcgtcc agacgacaaa accaatttta ctttcctgtc
    10861 ctattttcag aacgaaccag aaacgggtta ttacggttgg ttgccgaaag aggggaccgt
    10921 tgagccattg cctaatggta agcgtctacc gactgatttc aatgaaggtg catcgaataa
    10981 tacatactcc cgtaaccaga aaatggtggg atatagtttt gaacatggtt ttaatgacac
    11041 cttcaccgtg cgtcagaatc tgcgtttcag tgaaatgaaa acctcacaga aaagtgttta
    11101 tggcacaggg attgccagcg atggtcatac cttaaaccgc gggacagtgg tggataatga
    11161 gcgtctacaa aactttagcg ttgataccca acttgaaagt aagtttgcta caggtgacgt
    11221 cgggcatact ttgctgacag gggtagactt catgcgtatg cgcaatgata tcaatgccag
    11281 ctttggatct gcaccatcca tcgatcttta taacaaatac catcctgaat actttgcatt
    11341 tggtagcgca gagccatacc agatgaatga aagcaaacaa acaggtattt atgttcagga
    11401 tcaggcggaa tggaataaat gggtattcac tctgggggga cgctacgatt ggtctaagca
    11461 agcgaccact gttcgtgaaa actcttatac gccgactgaa ggttatattg agcgcaatga
    11521 tcatcagttc acctggcgcg gtggtgtaaa ttacttattc gataatggta tttcacctta
    11581 ctttagctat agccagtcct ttgaaccgag tgctttcgat ctgtggagca acccgcgcgt
    11641 ttcctataag ccatcgaaag gtgaacagta tgaagctggc gtaaaatatg ttccgaatga
    11701 tatgccggtc gttgttacgg gcgcagtcta tcaattgacg aaaacaaata acctgacagc
    11761 agacccaaca aacccattag cgcaagtccc ggcaggtgag attcgcgctc gtggtgtgga
    11821 acttgaagca aaagcggcgt taaatgccaa tattaacttg acggcttctt atacctacac
    11881 cgatgcggaa tacaccaaag acaccaatct caaaggtaaa actccagaac aagtaccgga
    11941 gcatatggca tctctctggg gggattatac cttcaatgaa gggccgcttt ctggtttaac
    12001 attgggaaca ggtggtcgtt ttattggttc cagctatggt gatccggcaa acacttttaa
    12061 agtgggtagc gcagctgtaa tggatgctgt tgtaaaatat gatctggcac gctttggtat
    12121 ggcgggatcc agccttgctg tgaacgtcaa caatttgctc gatcgtgagt atgttgccag
    12181 ttgcttccag acctatggct gcttctgggg cgcagaacgt caggtcgttg caaccgcaac
    12241 cttccgtttc taatttctct tttggggcac ggatttccgt gcccgtttca caagttggct
    12301 gttatgcagg aatacacgaa tcattccgat accacttttg cactgcgtaa tatctccttt
    12361 cgtgtgcccg ggcgcacgct tttgcatccg ctgtcgttaa cctttcctgc cgggaaagtg
    12421 accggtctga ttggtcacaa cggttctggt aaatccactc tgctcaaaat gcttggtcgt
    12481 caccagccac cgtcggaagg ggagattctt cttgatgccc aaccgctgga aagctggagc
    12541 agcaaagcgt ttgcccgcaa agtggcttat ttgccgcagc agcttcctcc ggcagaaggg
    12601 atgacagtgc gtgaactggt ggcgattggt cgttacccgt ggcatggcgc actagggcgc
    12661 tttggtgcgg cagatcgcga aaaggtcgag gaagctatct cgctggttgg cttaaaaccg
    12721 ctggcgcatc ggctggtcga tagtctctct ggcggcgaac gtcagcgggc gtggatcgcc
    12781 atgctggtgg cacaggatag ccgttgtctg ttgctcgacg aaccgacctc ggcgctggat
    12841 atcgcccacc aggttgatgt gctggcgctg gtgcatcgtt taagtcagga gcgtggcctg
    12901 acggtcattg ccgtgctgca cgatatcaat atggctgcgc gttactgtga ttatctggtc
    12961 gccctgcgcg gcggtgaaat gattgctcag ggaacgcctg cggaaattat gcgcggcgaa
    13021 accctcgaaa tgatttatgg catcccgatg ggtattttgc cgcatccggc gggtgctgca
    13081 cctgtgagtt ttgtttattg atgagcggct tacctcttat ttcgcgccgt cgactgttaa
    13141 cggcgatggc gctttctccg ttgttatggc agatgaatac tgcccacgcg gcggctattg
    13201 atcccaatcg tattgtggcg ctggagtggt tgccggtgga attactgctg gcgctcggca
    13261 tcgtgcctta cggcgtggcg gataccatca actatcgcct gtgggtcagc gaaccgccat
    13321 tgccggactc agtgattgac gttggtttgc gtacagaacc taaccttgaa ctgctgaccg
    13381 aaatgaaacc gtcgtttatg gtctggtcgg caggatatgg cccttcacca gaaatgctgg
    13441 cacgcattgc gccggggcgc ggatttaact tcagcgacgg caaacagccg ctggcgatgg
    13501 cgcgtaagtc actgacggaa atggcagatt tacttaacct gcaaagcgca gcagaaacgc
    13561 atttagcgca ctatgaagac tttatccgca gcatgaaacc ccgctttgtg aagcgtggtg
    13621 cgcgcccgtt attgctgacg acacttatcg atccgcgcca tatgctggtc ttcggtccaa
    13681 acagcttgtt ccaggaaatt cttgatgagt acggcatccc aaatgcctgg caaggggaaa
    13741 ccaacttctg gggcagtacc gccgtcagta tcgatcgtct ggcagcgtat aaagacgttg
    13801 atgtgctctg ttttgatcac gacaacagca aagacatgga tgcgctaatg gcaaccccgc
    13861 tgtggcaggc catgccgttt gtccgtgcag gacgttttca gcgcgttccg gcggtctggt
    13921 tctatggcgc gacgctttcg gcaatgcact ttgtgcgtat tctggataac gccatcggag
    13981 gtaaagcgtg agtaaacgaa ttgcgctttt cccggcgtta ttgctggcgc tgctggttat
    14041 tgtcgcagcg gcgctcacct ggatgaactt ttcgcaagcg ctgccgcgta gccagtgggc
    14101 gcaggctgcc tggtcgccgg atattgacgt catcgagcag atgatttttc actacagctt
    14161 gttgccgcgt ctggcgattt cgctgctggt gggcgcgggc ctggggctgg tgggcgtgct
    14221 gtttcagcaa gtgctgcgta acccgctggc ggaaccgacg acgttgggcg ttgcaacagg
    14281 cgcgcaactg gggattaccg tcactacgct ctgggcgatc ccaggtgcta tggcgagcca
    14341 gtttgctgcg ctggcagggg cttgcgttgt tggcttaatt gtctttggcg tcgcgtgggg
    14401 gaaacggctg tcgccggtaa cgctgattct cgcggggctg gtagtgagcc tttattgtgg
    14461 ggcaatcaat cagttactgg ttatcttcca tcatgaccag ttgcaaagca tgttcctgtg
    14521 gagcaccgga acgctgacgc aaaccgactg gggcggcgtt gagcgtttat ggccgcagtt
    14581 gctgggcggc gtgatgctga cgttactgct acttcgtccg ttaaccctga tggggcttga
    14641 tgatggcgtg gcgcgcaacc tcgggctggc cttgtcgctc gcgcgtctgg cggcgctgtc
    14701 gctggcgata gtcatcagtg cgctgctggt gaacgcagtg gggattatcg gctttatcgg
    14761 gttgttcgcg ccactgctgg caaaaatgct gggggcgcgg cgtttgttgc cacgattgat
    14821 gctggcgtcg ctgattgggg cgttaattct gtggctttct gatcaaatca tcctctggct
    14881 gactcgcgtg tggatggaag tttctaccgg ttcggtcact gcgttgatag gtgcgccgct
    14941 gctactttgg ctgctgccgc gtttacgcag cattagcgcg ccggatatga aggtgaacga
    15001 tcgtgtcgct gctgaacgcc aacatgtgct ggcgtttgcc ctcgcgggcg gcgtgctgct
    15061 gttgatggca gtggtggtgg cgctgtcgtt tggccgtgat gcgcacggct ggacgtgggc
    15121 gagcggggcg ttgctcgagg atttgatgcc ctggcgctgg ccgcgaatta tggcggcgct
    15181 gtttgcggga gtcatgctgg cggtggcggg ctgtattatt cagcggctga ccggaaaccc
    15241 gatggcaagc ccggaagtgc tggggattag ctccggcgcg gcgtttggcg tggtgttgat
    15301 gctgtttctg gtgccgggta atgcctttgg ctggctgtta cctgcaggca gtctcggcgc
    15361 ggcggtgacg ctgttgatca ttatgatcgc cgccggacgc ggtggatttt ccccacaccg
    15421 catgttactg gcggggatgg cgttaagtac cgcgttcacc atgcttttga tgatgttgca
    15481 ggcaagtggt gacccgcgaa tggcgcaagt tctgacctgg atttccggtt cgacctacaa
    15541 cgcgaccgat gcgcaggtct ggcgtaccgg aattgtgatg gtgattttgc tggcgattac
    15601 cccgctgtgc cgccgctggc tgaccatttt accgctggga ggtgataccg cacgagccgt
    15661 ggggatggcg ctgacgccga cgcgaattgc gctgctgctg ttagcggctt gcctgacagc
    15721 gactgcgaca atgaccattg gaccgttgag ttttgttggt ttaatggcac cacatattgc
    15781 gcggatgatg ggctttcgac ggacgatgcc acacatcgta atttcggcgc tggtgggagg
    15841 attactgctg gtgttcgctg actggtgtgg gcggatggtg ctgtttccat tccagatccc
    15901 agcggggctg ctgtcgacct ttatcggcgc gccatatttt atctatttgt tgagaaagca
    15961 gagccgttaa ttgaatgcct cgcccggtgg gcgaggcaaa atcatcacaa cttcgcaaac
    16021 acccgacgtg cagcatcgat ggtgttattg atatcttcca tgctgtgcgc cacagacata
    16081 aagcccgctt caaacgctga cggtgccaga taaacacctt cgtccagcat catatggaag
    16141 aaacgcttaa agcgttccac gtcacaggcc atcacatcct gatagcacgt cacggactcg
    16201 gcgtcggtaa agaaaatacc gaacatgccg ccaacgtggt ttaccaccag cggaattccg
    16261 gcttcttctg ccgcttccag cagaccttct gccagacgtg ttgtcagctc atccagcgtt
    16321 tcgtgaacgc ccggctgcgc gacttcattc agacaggcga aaccagcagc catcgcaatc
    16381 gggttaccgg aaagcgtacc cgcctgatag accggaccgg tcggggccag cgcatccatt
    16441 acatcacgac gaccaccgaa tgcgcctacc ggcattccac cgccgatgat tttgccgagg
    16501 caggtgagat ccggttccac accgtaataa tcctgtgcgc cagctagcgc tacgcggaaa
    16561 ccggtcatca cttcatcgat gatcagcaac gcgccaaatt cgtcgcacag cgcgcgcaga
    16621 cctggcagga actctggcag cggtggaacg cagttcatat tgcctgccac cggctcgacg
    16681 ataatacagg caatctcttg cgggtattgc tcaaacgcgg cgcgtacaga ggccagatcg
    16741 ttataagtac aggttaaggt atgtttggcg aaatctgccg gaacgcccgg cgaatttggc
    16801 tggcctaacg tgagtgcgcc agaaccggct ttcaccagca ggcagtcagc gtgaccgtgg
    16861 taacaacctt caaatttaat aattttgtcg cgaccggtaa aaccacgggc caggcggatg
    16921 gcactcatgg tcgcttcagt gccggagttc accatgcgca ccatatccat ggtcgggacc
    16981 agttcggtca ccagttgcgc cattttcact tccatttcgg tcggtgcacc aaagcttaaa
    17041 ccacgctcgg cggcttcaat cacggcattg cggatcgccg gatggttatg gcccagcacc
    17101 atcggccccc aggaaccgac ataatcgata taggctttgc catcaacatc gtacagataa
    17161 gcgccgtccg ctttttcgat aaacagtgga gtgccgccca cgccagtaaa ggcgcgaaca
    17221 ggggagttca caccgccagg gatcagctcg cgcgctgcgc tgtaaagatt ttcagactta
    17281 ctcatggagg gttcctgatt cgtagaaaaa gtgaatggct gctattctat gttattcata
    17341 acaagttaaa tactcgtcaa acatcaggct gcttgtactg gtgcaatcct gatttcttag
    17401 agtataaaag ttttgtgcat ttgaaacatt acgctttgca aaggattttc atggaacgtg
    17461 cgagtaaaat gccgtcatct tatttgtatg accaataagt gatcattgga tgaaaactga
    17521 tactccctct ttagaaacac cgcaggccgc gcgcctgcga cgcagacaac tgattcgcca
    17581 acttcttgag cgcgataaaa ccccgttagc cattttgttt atggcggcag tcgtcggcac
    17641 gcttgtcggg ctggctgcgg ttgcttttga caaaggtgtc gcctggttgc agaaccagcg
    17701 tatgggggcg ctggtacata ctgctgataa ttatccgctt ctgttaaccg tcgcttttct
    17761 ctgttcggcg gtgctggcga tgttcggcta ctttttggtg cgcaaatacg cgccggaagc
    17821 aggtggttcg gggatcccgg aaattgaagg ggcgctggaa gatcaacgtc ccgttcgctg
    17881 gtggcgtgtg ttgccggtga aattctttgg cgggctgggg acactcggcg gaggcatggt
    17941 gttggggcgc gaagggccaa ccgtacagat cggcggcaat atcggtcgca tggtgcttga
    18001 tattttccgc ctgaaaggtg atgaagctcg ccatacgctg ttggcaaccg gtgcggcggc
    18061 gggtctggcg gcggccttta acgccccgct agcgggtatt ttgtttatta tcgaagagat
    18121 gcgtccgcag tttcgctata cgttaatttc gattaaagcg gtatttattg gcgtcattat
    18181 gtcgaccatt atgtaccgga tttttaatca tgaagttgca ttgattgacg tcggtaaact
    18241 ttctgacgcg ccgcttaata cgctgtggct ttatctgatc ctcggtatta tttttggcat
    18301 tttcggccct atttttaata aatgggtgct ggggatgcag gatttgctgc accgtgttca
    18361 cggcggcaat attaccaaat gggtactgat gggcggtgta attggcggtc tgtgtggatt
    18421 gctggggttt gtggcaccag caacgtcggg cggcggcttt aacctgattc ctatcgctac
    18481 cgcagggaat ttcagcatgg gaatgctggt gtttatcttc gtcgcgcggg tcattaccac
    18541 cttactctgt ttctcttccg gcgcgccggg cggtattttt gccccgatgc tggcgctggg
    18601 tactgtgctg ggaaccgctt tcggaatggt tgccgttgag ctgtttccgc aatatcacct
    18661 tgaagcaggc acttttgcta ttgccggaat gggggcgtta ctggcggcat ctattcgcgc
    18721 gccgttaacg ggaatcattc tggttctgga aatgaccgat aactaccagc tcattttgcc
    18781 aatgattatt accggtcttg gcgcaacact attagcacaa tttaccggcg ggaaaccgct
    18841 atactcggcg attcttgcgc gcacgctggc aaaacaggaa gctgagcaac tggcgcgaag
    18901 caaggccgca tcagccagcg agaatacttg aacgaaatac cagggtatta gataatggcg
    18961 attattattg ggttagaatt tgcccaattg ccgatgtcgt ttggagcaaa atatgagtga
    19021 tgacgtagca ctgccgctgg agtttaccga cgcagcagcc aacaaagtta aaagcctgat
    19081 cgctgacgaa gataacccga atctgaaatt acgcgtgtat atcaccggtg gcggttgcag
    19141 cggcttccag tatggtttca cctttgatga tcaggtgaac gaaggcgata tgaccatcga
    19201 aaaacagggc gttggcctgg tggttgatcc gatgagcctg caatatctgg tcggcggttc
    19261 cgttgactat accgaaggtc tggaaggttc tcgtttcatc gtgaccaacc cgaacgcgaa
    19321 aagcacctgc ggttgcggtt cttcctttag catctaatct gttgttcatg tgccggataa
    19381 actatccggc acatcttctc tcttcaacgc ccattctcat ccagcgcaaa cgtcggtagc
    19441 ttaagatgcc aacgaatagc cgccagccga atcaatagcg tcacgaccat gcccatcata
    19501 ctggctgttt ccagtggtac ggaaaatgtg taataagccg tagcgtggac aataccgccg
    19561 ataatacagg cagttgcgta aatttctgta cgtaaaatca tggggatttc gcgggccagt
    19621 acatcacgaa tgatcccgcc gccaacgcca gtaatgacgc ccatacaaac cgcgattaac
    19681 ggaccggctt ccgcattaaa ggctttattc acgccaatgc cgacaaacac cgccagacca
    19741 acggcgtcca gcaccggcaa catccatttt ggtaagcgtc ttggctggcg caccagcacg
    19801 atggtcagca tgctggtgac cattgcaacg accagatcgg tgggatcttt cacccaaaat
    19861 accgggccgt gatccagcgc catgtcgcga attgtcccgc cgcctactgc ggtaaccacg
    19921 cccagtacca gaacaccaaa agggtccata cgcaattttc cggctaacaa aacgccggag
    19981 atggcaaata ccgctgtgcc gactatatcc agccaataga cgagcattgt tcaatcccca
    20041 ctgagcacct gaaaaggtca ggcgctaatc tacctgtgaa agcgcattac agagctgttg
    20101 tgcagcgagg ataatacgtg ggcttgcacg ttcaaaccag tcactcgtga gaggaataac
    20161 gggaattttg agctgttcac cccagtattg tttgatttta ggaatttggt ccggtccgcc
    20221 tgtaatgact atcgcctgtg gcgcgcgtgc taacacctgt tcgcggctaa cttgcggcca
    20281 gggaacccgg ctatctttaa agatgttttc tccgccacaa acttcgagta cctggttctg
    20341 aatcgactct tttccactgg taaatggcgg attaatgccg aattgcagaa aaacacgttt
    20401 tttaggttta tcagcatatt gcgctttcaa ttgcgcgtac tgatccagca gggattgcgc
    20461 ggcttgttcg gccttgtctg gttgcggact ccagggggcc agttgacgta acgcattggc
    20521 aatttgttca atgcttgtcg catcgaccca catcactttt attcccagcg aagccagctg
    20581 gtcaacctgc cgctcggcat tacctccacg ccaggcaatc accagatcgg gtttcagcgc
    20641 gacaatgcgt tccagattca tcccctgcca ggtggaaacc tgctcaatct tttgcgcttg
    20701 tggaggatag tcggaatagc tgctgacccc aaccggcgtg atcccggcgg caaaggcaag
    20761 ttcagtgttg gcgggagaaa gcgtgatgac gcgcggcgcg gcgttgagcc acagtggcgc
    20821 aagaaaagac agggcgacca gcgccctgaa cagtgactta gccatgtgcc agtttctgca
    20881 ccagtgactc aaccatcagg ctggactgtt tagcggcaac agccaggaac tcatcgaagc
    20941 taagatgaga ctgttgatcg gccacgtcgg agatggcgcg tactacgaca aacgggacgt
    21001 tgaaattgtg gcagacatgg gcgattgccg tcgcttccat ctctacagca atggcctgtg
    21061 ggaagttgtg gcggattttt gccagaccaa cagaaccgtt gatgaaagcg tcgccgctga
    21121 caatcaggcc acgtacagcg ttaagattca gttcggcaat gcaggcctca gcggcagcga
    21181 tcagtttatc gtcagcttta aagcctgccg gacagcctgg taactgaccg tattcataac
    21241 caaatgccgt gacatccgcg tcgtgataac gtgcttcgtc cgagacaacg atatcgccca
    21301 ctttcagcgt tggtgccagg ccaccggcag aaccggtgtt aataatcaca tctggcttgc
    21361 agtgttccaa cagcagagtg gcacccagcg ccgcagcgac tttaccgatg cccgatttca
    21421 gaagcgcaac ctcggttcca ttcagttggc cggtatagat ttcgcaaccg ccgagactga
    21481 tagtttgacg gttttcgatt ttgtcacgca gcagcgtaac ttcttcttcc attgcaccaa
    21541 tgatgccgat tttcatagat ttactcgcga taagcccgat ttgaaggcat agtttaccat
    21601 gcgcttacgg ggaagcgtat ttctcacgcg ggagaggaca tggcacagat tgatttccga
    21661 aaaaaaataa actggcatcg tcgttaccgt tcaccgcagg gcgttaaaac cgaacatgag
    21721 atcctgcgga tcttcgagag cgatcgcggg cgtatcatca actctccggc aattcgtcgt
    21781 ctgcaacaaa agacccaggt ttttccactg gagcgcaatg ccgccgtgcg cacgcgtctt
    21841 acccactcga tggaagtcca gcaggtgggg cgctacatcg ccaaagaaat tttaagccgt
    21901 ctgaaagagc ttaaattgct ggaagcatac ggcctggatg aactgaccgg cccttttgaa
    21961 agcattgttg agatgtcatg cctgatgcac gatatcggca atccgccgtt tggtcatttt
    22021 ggcgaagcgg cgataaatga ctggtttcgc cagcgtttgc acccggaaga tgccgaaagc
    22081 cagcctctga ctgacgatcg ctgcagcgtg gcagcactac gtttacggga cggggaagaa
    22141 ccgcttaacg agctgcggcg caaaatacgt caggacttat gtcattttga ggggaatgca
    22201 caaggcattc gcctggtgca tacattgatg cggatgaatc tcacctgggc acaggttggc
    22261 ggtattttaa aatatactcg tccggcgtgg tggcgtggtg aaacgcctga gacacatcac
    22321 tatttaatga aaaagccggg ttattatctt tctgaggaag cctatattgc ccggttgcgt
    22381 aaagaactta atttggcgct ttacagtcgt tttccattaa cgtggattat ggaagctgcc
    22441 gacgacatct cctattgtgt ggcagacctt gaagatgcgg tagagaaaag aatatttacc
    22501 gttgagcagc tttatcatca tttgcacgaa gcgtggggcc agcatgagaa aggttcgctc
    22561 ttttcgctgg tggttgaaaa tgcctgggaa aaatcacgct caaatagttt aagccgcagt
    22621 acggaagatc agttttttat gtatttacgg gtaaacaccc taaataaact ggtaccctac
    22681 gcggcacaac gatttattga taatctgcct gcgattttcg ccggaacgtt taatcatgca
    22741 ttattggaag atgccagcga atgcagcgat cttctaaagt tatataaaaa tgtcgctgta
    22801 aaacatgtgt ttagccatcc agatgtcgag cagcttgaat tgcagggcta tcgggtcatt
    22861 agcggattat tagagattta tcgcccttta ttaagcctgt cgttatcaga ctttactgaa
    22921 ctggtagaaa aagaacgggt gaaacgtttc cctattgaat cgcgcttatt caacaaactc
    22981 tcgacgcgcc atcggctggc ctatgtcgag gctgtcagta aattaccgtc agattctcct
    23041 gagtttccgc tatgggaata ttattaccgt tgccgcctgc tgcaggatta tatcagcggt
    23101 atgaccgacc tctatgcctg ggatgaatac cgacgtctga tggccgtaga acaataacca
    23161 ggcttttgta aagacgaaca ataaattttt accttttgca gaaactttag ttcggaactt
    23221 cgcgctataa aacgaatctg aagaacacag caattttgcg ttatctgtta atcgagactg
    23281 aaatacatga aaaaaaccac attagcactg agtgcactgg ctctgagttt aggtttggcg
    23341 ttatctccgc tctctgcaac ggcggctgag acttcttcag caacgacagc ccagcagatg
    23401 ccaagccttg caccgatgct cgaaaaggtg atgccttcag tggtcagcat taacgtagaa
    23461 ggtagcacaa ccgttaatac gccgcgtatg ccgcgtaatt tccagcagtt cttcggtgat
    23521 gattctccgt tctgccagga aggttctccg ttccagagct ctccgttctg ccaggggggc
    23581 cagggcggta atggcggcgg ccagcaacag aaattcatgg cgctgggttc cggcgtcatc
    23641 attgatgccg ataaaggcta tgttgtcacc aacaaccacg ttgttgataa cgcgacggtg
    23701 attaaagtcc agctgagcga tggtcgtaag ttcgacgcga agatggttgg caaagatccg
    23761 cgctctgata tcgcgctgat ccagatccag aacccgaaaa atctgaccgc aattaagatg
    23821 gcggattctg atgcactgcg cgtgggtgat tacaccgtag cgattggtaa cccgtttggt
    23881 ctgggcgaga cggtaacttc cgggattgtc tctgcgctgg ggcgtagcgg cctgaatgct
    23941 gaaaactacg aaaacttcat ccagaccgat gcagcgatca accgtggtaa ctccggtggt
    24001 gcgctggtta acctgaacgg cgaactgatc ggtatcaaca ccgcgatcct cgcaccggac
    24061 ggcggcaaca tcggtatcgg ttttgctatc ccgagtaaca tggtgaaaaa cctgacctcg
    24121 cagatggtgg aatacggcca ggtgaaacgc ggtgagctgg gtattatggg gactgagctg
    24181 aactctgaac tggcgaaagc gatgaaagtt gacgcccagc gcggtgcttt cgtaagccag
    24241 gttctgccta attcctccgc tgcaaaagcg ggcattaaag cgggtgatgt gatcacctca
    24301 ctgaacggta agccgatcag cagctttgcc gccctgcgtg ctcaggtggg tactatgccg
    24361 gtgggcagca aactgaccct gggcttactg cgcgacggta agcaggttaa cgtgaacctg
    24421 gaactgcagc agagcagcca gaatcaggtt gattccagct ccatcttcaa cggcattgaa
    24481 ggcgctgaga tgagcaacaa aggcaaagat cagggcgtgg tagtgaacaa cgtgaaaacg
    24541 ggcactccgg ctgcgcagat cggcctgaaa aaaggtgatg tgattattgg cgcgaaccag
    24601 caggcagtga aaaacatcgc tgaactgcgt aaagttctcg acagcaaacc atctgtgctg
    24661 gcactgaaca ttcagcgcgg cgacagcacc atctacctgt taatgcagta atctccctca
    24721 accccttcct gaaaacggga aggggttctc cttacaatct gtgaacttca ccacaactcc
    24781 atacatcttc atcatccttt gggcatttgc acaatgccgt acgttacgta cttccttatg
    24841 ctaagccgtg cataacggag gacttatggc tggctggcat cttgatacca aaatggcgca
    24901 ggatatcgtg gcacgtacca tgcgcatcat cgataccaat atcaacgtaa tggatgcccg
    24961 cgggcgaatt atcggcagcg gcgatcgtga gcgtattggt gaattgcacg aaggtgcatt
    25021 gctggtactt tcacagggac gagtcgtcga tatcgatgac gcggtggcac gtcatctgca
    25081 cggtgtgcgg caagggatta atctaccgtt acggctggaa ggtgaaattg tcggcgtaat
    25141 tggcctgact ggtgaaccag agaacctgcg taaatatggc gaactggtct gcatgacggc
    25201 tgaaatgatg ctggaacagt cgcggttgat gcacctgttg gcgcaggata gccgtttgcg
    25261 ggaagaactg gtgatgaacc tgattcaggc tgaggagaat acccccgcac ttactgaatg
    25321 ggcgcaacgg ctggggatcg atctcaatca accgcgagtg gtggctattg ttgaggtcga
    25381 cagcggtcag cttggcgtgg acagcgcaat ggcggagtta cagcaactgc aaaacgcgct
    25441 gactacgcct gagcgtaata atctggtggc aattgtttcg ctgaccgaaa tggtggtgtt
    25501 gaaaccggcg ttgaactctt ttgggcgctg ggatgcagaa gatcatcgta agcgagttga
    25561 acaactgatt acccgcatga aagagtacgg ccagctgcgt tttcgcgttt cgctgggtaa
    25621 ctattttacc ggaccgggca gtattgcccg ctcttatcgt acggcgaaaa cgacgatggt
    25681 ggtgggtaaa cagcgaatgc cagaaagtcg ctgctatttt tatcaggatc tgatgttgcc
    25741 tgtgttactc gacagtttgc gtggcgactg gcaggccaac gaactggcgc gaccgctggc
    25801 gcggctgaaa gcgatggaca ataacggctt gctgcgacga acgctggcgg cgtggtttcg
    25861 ccacaatgtg caaccgctgg ctacgtcaaa ggcgctgttt attcatcgta atactctgga
    25921 gtatcggctt aatcgtatat cggaactgac cgggctggat ttgggcaatt ttgatgacag
    25981 gttgctgctg tatgtggcgt tgcaactgga tgaagagcgg taggtcgatg acaaaattac
    26041 agccgggtga gactttaccc ggcccaaaga atggcattat ttacgcgtca gtttttccag
    26101 atcggcttca atctcgctga tcttattggt tactactgac tccagatgac gtaagtcgtc
    26161 gaggatctta cgcttaagat caacttcgct gcggtcacgc tggcagattt gatctaactc
    26221 atcaatgata tagcgaagat tagggctaat ctcctgcact tctttataac cctgacccac
    26281 accatcggcg acgaccgttt tacgctgacg cggatattta aacttcacgc tcttggcgaa
    26341 aaactcgcct ttgtctttct ggaaatagat tttcagaata tcgttgttgg cttcctgccg
    26401 gaggctgtaa cgatcaattt cttcaggatt ggtaataccc agacttttca gattgtcgta
    26461 catagcgtta cctcaaaatg agtcagtaaa ttgtgcttat tttagcattt ggcctggccc
    26521 gcccggctcg actgtgatcg cagaaagcct ttttcttcca gacttctatc atggcgtaaa
    26581 aatcaaaaaa ttacctgctt tattgtggcg atattccatg ggatggatta gcacatttat
    26641 ctttgttttg cgaattacat gacagtccga aacaagatga aaaagtattt aaaaaagggg
    26701 aagaagaatg agtaggacga attattctga accagaaagc aatcgggcag cgtcgtgctg
    26761 cccgtgtgca tacttttagt cgatggtacg cagcagttcg ttaatgccga ctttgccgcg
    26821 agttttcgcg tcaactttct taacgataac cgcacagtaa agactgtatt tgccatcttt
    26881 cgacggcaga ttgcctgaaa caaccacaga tcctgccgga acgcgaccgt agtggatttc
    26941 gccggtttca cggtcgtaaa tacgggtgct ctgaccaatg tatacgccca tagagatgac
    27001 ggaaccttct tcgacaatta ccccttcaac cacttcagag cgcgcgccga tgaagcagtt
    27061 atcttcaata atggtcgggt tagcctgcag cggctccagc acgccgccga tacccacgcc
    27121 accggaaagg tggacgtttt taccaatctg cgcacaggaa ccgacggtcg cccaggtatc
    27181 aaccatcgtg ccttcatcaa catatgcgcc gatgttgacg taagacggca tcagcacggt
    27241 gttacgggca atgaacgcgc cctgacgcac tgccgctggt ggcacgacgc ggaagccttc
    27301 tttctggaaa cgtgcttcat cgtagttagc gaatttcatc ggcactttgt cgaagtagcg
    27361 gctttctgcc ccttcgatca cctgattatc attaatacgg aaagagagca gcaccgcttt
    27421 tttcaaccac tgatgcgtca cccactgacc gtcaattttt tccgctacac gcagtgcgcc
    27481 ggaatccagc agggcgatca cctgatttac cgcttcgcgg gtaactgtgt ctgcattggc
    27541 tggcgtgatc tcggcacggc gttcaaaagc ggtttcaata atgttctgta actgctgcat
    27601 tgttaaactc ttttcatatc agtaaacaca tcacccttta tcgtttggat tgagggcctc
    27661 tgtcaaccgc tgatgcactt cctgctgcaa ctcgttatta agtgcacgcc ggtcagcggt
    27721 ggcaattatg aataaatctt ctactcgttc gccaatggtt gtaattcggg cgccatgaag
    27781 cgaaattccc agatcggcaa aaattttccc gactctcgcc agcagtcctg gttggtcgag
    27841 cgcgataagc tcaaggaacg atttgcggtc ggtatgggtc ggcaaaaatg tcacttcagt
    27901 ttcaacagta aagtgacgta gtttggccgg ttggcgacgt ggctgcggtg gctgccagct
    27961 actttgtgtc agtacctgct ccagaccaaa tcgaataact tcatggcgat ctgcggacag
    28021 cgggctgcca tcgggttcca gcacgatgaa ggtatccatc gccataccgt cgcgagtggt
    28081 gaaaatttgt gcgtcgtgga cacttaaatt gcggcggtct aattcggcac agacggcggc
    28141 aaacagatag gggcggtccg ggctccagat aaaaatctcg gtgcctccac gcgtagcctg
    28201 cggactaagc aataccagcg gtttgcttaa atcatgctgt aataaatggc gcgcatgcca
    28261 ggccagttga tttgggctat ggcggacaaa atagttagca cgacagcgtg accaaatttg
    28321 gtgcagcgcc tcttcgtcga tgttatccat gcgcagtagt gccagcgcct ggagttggtg
    28381 atggcgaacc cgttcgcgca tatccggcgt gttttgcatt ccacgtcgta gctgcttttc
    28441 ggtggcgaaa tagagttcgc gcaacagact ttgcttccag ctattccaca gcgtttcgtt
    28501 ggtggcgcaa atgtcagcca ctgtcaggca taccagatag cgcagacgat tttccgtttg
    28561 aacttcttcg gcaaactgct tgatcacttc cgggtcctga atatcgcggc gctgagctgt
    28621 caccgacatc agcaagtgct ggcgaaccag ccacgccacc agctgtgttt cgcgcgagtt
    28681 aagcccgtgg agttcggcaa aatgcaccac gtcttgtgca ccgagaatgg agtggtcgcc
    28741 gccgcgtcct ttggcgatat cgtgaaacag cgcggcgatg aaaatcagct cagttgacgg
    28801 caggcgcggc cagacgtcca cacacaacgg atggcgctgg cgcgtttctt cactggcaaa
    28861 actctccagt ttcagcatca cgcgaatagt atgttcatcc accgtgtagg cgtggaacag
    28921 gtcaaactgc atctgcccga cgatatgcga ccattggggc atatacgcgc cgagcacgct
    28981 atgacgatgc atcggtaata gtccgcgacg caccgcacca gggtgacgca gaatgctcaa
    29041 aaacagtttt cgcgcttccg gaatattaca cagcggttgt tgcaaatggc gacgggcgtg
    29101 gcgtaactgg cgcagcgtgg tagagtaaat gccagtgatc gcactgttgc gcaccatggt
    29161 gtagaacata cgcaagatgg cttccggctg gcgcataaat agtgtttcat cacgcaggtc
    29221 gattagcgta ccgcgtagct gaaactcatc gtcgattgga cgtggttttt cgtcggcggg
    29281 aagggcgagg atggcttcat cgaacagttg cagcagcatc tggttgagtt cgctgacgcg
    29341 gcgtgtaacg cggaagtaat ccttcatcat ccgctcgacc ggttcgttac cttcaccact
    29401 gtaattcaaa cgctgggcga cgctaagctg gcgatcgaat aacaggcgat tatcgtaacg
    29461 gctgacgaca agatgcaggg caaagcgaat acgccacaat atatgcagac attcgtttaa
    29521 ttccgctcgc tccgctgagg ttaagaagcc aaacccgacc atttcatcca acgatgttgc
    29581 gccaaaatga cggcgggcca cccattgcag cgtgtggata tcgcgcaagc cgccggggct
    29641 acttttgatg tctggttcaa ggttgtagct ggtgccgtgg taacgctgat ggcgctggtt
    29701 ctgttcttca actttcgccg cgtagaactt gtcggaaggc cagaatcctt cgctgaaaat
    29761 atgtttttgc agttcgagga acagcgcaac atcgccaatt aataagcggg attcgattaa
    29821 attggtggcg acggttaaat ccgataaccc ttccagcatg cactcttcaa gcgtgcgcac
    29881 gctatgaccg acttccagtt tcacatccca taatagtgtt aacagttcgc caactttctg
    29941 tgcctgatca tccgggagtt tcttacggct taagatcagc aaatcgatgt cggaaagtgg
    30001 atgcagctcg ccacgaccgt agccaccgac ggcgactaat gccaggtcgg caatctggct
    30061 gaatcccgcg tcaatccata accgttgcag gagctggtcg ataaactcgg tacgcgcctc
    30121 aatcaactct tctgcagaga ttccattgtc aaaggcatca cccagccaac gctggaaagt
    30181 atcaatatgg gcttttatcc cacagacggt taattcatca cggggccagg cacatggatt
    30241 ttgcggttga ccgggaaggg tggggagagc ggtatttgcg tactgttctg gaagggtatt
    30301 catcgtgcgc cacccataag attaaattat cacattaaaa aagccggcat tcgccggctt
    30361 catcttattc gtcgtgcgag attatcgccg ggatggtgtc atccttgcgt agcgtcagaa
    30421 tttcgcagcc gttatcagtc accacaatag tatgctcata ttgtgcagac aagctgcgat
    30481 ctttggtttt taccgtccag ccatctttca tggtgcggat ctctttttta cccgcgttga
    30541 ccattggctc gatggtgaag gtcatccccg gtttcagcac gacgttggtt tcaggggagt
    30601 catagtgcag tacctgcggt tcttcatgga agccacgacc aataccgtgt ccacaatatt
    30661 cacgaacgac ggagaaacct tctgcttcaa caaatttctg aatcgccgca ccgatttcgc
    30721 gcagattaat gcctggtttg accatgcgca gcgccaggta caggctttct tgcgtgatgc
    30781 ggcacagacg ttcgcccatg atggtcggct taccgacgat aaacattttc gaggtatcgc
    30841 cgtggaaacc atctttgatt acggtgacat caatgttaac gatatcgcca tctttcagca
    30901 gcttggcgtc gtccgggata ccgtggcaca ccacttcatt aatagagatg cagacggatt
    30961 tcggatagcc gtgatagccg aggcaggcag aaaccgcgtg ttgttcatta acaatgtaat
    31021 cattacagat gcgatccagc tcgccggtgc tgacgcccgg tttaacatac ggttcgatca
    31081 tctccagcac ttcggcagcc agtcggccag cgacgcgcat tttttcgata tcttctgggg
    31141 tcttgattga gatagccatt aattctgtcc atcagcgtcg gtgataccga caatatatat
    31201 gtaagtgccg tcaatggtat cacacccggg caaattgaga atcattctga atttcgccaa
    31261 acgtgccact gaaggttttc tataatagaa aattcaacgt ctgatgctgt acacagcgcc
    31321 aacaattatt ggtgtccgcg acgtatttgt ggtataaagc gcgccggact tccgatccat
    31381 ttcgtataca cagactggac ggaagcgaca atctcacttt gtgtaacaac acacacgtat
    31441 cggcacatat tccggggtgc cctttggggt cggtaatatg ggatacgtgg aggcataacc
    31501 ccaactttta tatagaggtt ttaatcatgg caactgtttc catgcgcgac atgctcaagg
    31561 ctggtgttca cttcggtcac cagacccgtt actggaaccc gaaaatgaag ccgttcatct
    31621 tcggtgcgcg taacaaagtt cacatcatca accttgagaa aactgtaccg atgttcaacg
    31681 aagctctggc tgaactgaac aagattgctt ctcgcaaagg taaaatcctt ttcgttggta
    31741 ctaaacgcgc tgcaagcgaa gcggtgaaag acgctgctct gagctgcgac cagttcttcg
    31801 tgaaccatcg ctggctgggc ggtatgctga ctaactggaa aaccgttcgt cagtccatca
    31861 aacgtctgaa agacctggaa actcagtctc aggacggtac tttcgacaag ctgaccaaga
    31921 aagaagcgct gatgcgcact cgtgagctgg agaaactgga aaacagcctg ggcggtatca
    31981 aagacatggg cggtctgccg gacgctctgt ttgtaatcga tgctgaccat gaacacattg
    32041 ctatcaaaga agcaaacaac ctgggtattc cggtatttgc tatcgttgat accaactctg
    32101 atccggacgg tgttgacttc gttatcccgg gtaacgacga cgcaatccgt gctgtgaccc
    32161 tgtacctggg cgctgttgct gcaaccgtac gtgaaggccg ttctcaggat ctggcttccc
    32221 aggcggaaga aagcttcgta gaagctgagt aataaggctt gataactccc ccaaaatagt
    32281 tcgagttgca gaaaggcggc aagctcgaga attcccggga gcttacatca gtaagtgacc
    32341 gggatgagcg agcgaagaca acgcatctgc ggcgcgaaat atgaaggggg agagccctta
    32401 tagaccaggt agtacacgtt tggttagggg gcctgcatat ggcccccttt ttcactttta
    32461 tatctgtgcg gtttaatgcc gggcagatca catctccgag gattttagaa tggctgaaat
    32521 taccgcatcc ctggtaaaag agctgcgtga gcgtactggc gcaggcatga tggattgcaa
    32581 aaaagcactg actgaagcta acggcgacat cgagctggca atcgaaaaca tgcgtaagtc
    32641 cggtgctatt aaagcagcga aaaaagcagg caacgttgct gctgacggcg tgatcaaaac
    32701 caaaatcgac ggcaactacg gcatcattct ggaagttaac tgccagactg acttcgttgc
    32761 aaaagacgct ggtttccagg cgttcgcaga caaagttctg gacgcagctg ttgctggcaa
    32821 aatcactgac gttgaagttc tgaaagcaca gttcgaagaa gaacgtgttg cgctggtagc
    32881 gaaaattggt gaaaacatca acattcgccg cgttgctgcg ctggaaggcg acgttctggg
    32941 ttcttatcag cacggtgcgc gtatcggcgt tctggttgct gctaaaggcg ctgacgaaga
    33001 gctggttaaa cacatcgcta tgcacgttgc tgcaagcaag ccagaattca tcaaaccgga
    33061 agatgtatcc gctgaagtgg tagaaaaaga ataccaggta cagctggata tcgcgatgca
    33121 gtctggtaag ccgaaagaaa tcgcagagaa aatggttgaa ggccgcatga agaaattcac
    33181 cggcgaagtt tctctgaccg gtcagccgtt cgttatggaa ccaagcaaaa ctgttggtca
    33241 gctgctgaaa gagcataacg ctgaagtgac tggcttcatc cgcttcgaag tgggtgaagg
    33301 catcgagaaa gttgagactg actttgcagc agaagttgct gcgatgtcca agcagtctta
    33361 attatcgaaa aggagccgcc tgagggcggc ttctttttgt gcccatcttg taaattcagc
    33421 taacccttgt ggggctgcgc tgaaaagcga cgtacaatgt cgctagtatt aattcatttc
    33481 aatcgttgac agtctcagga aagaaacatg gctaccaatg caaaacccgt ctataaacgc
    33541 attctgctta agttgagtgg cgaagctctg cagggcactg aaggcttcgg tattgatgca
    33601 agcatactgg atcgcatggc tcaggaaatc aaagaactgg ttgaactggg tattcaggtt
    33661 ggtgtggtga ttggtggggg taacctgttc cgtggcgctg gtctggcgaa agcgggtatg
    33721 aaccgcgttg tgggcgacca catggggatg ctggcgaccg taatgaacgg cctggcaatg
    33781 cgtgatgcac tgcaccgcgc ctatgtgaac gctcgtctga tgtccgctat tccattgaat
    33841 ggcgtgtgcg acagctacag ctgggcagaa gctatcagcc tgttgcgcaa caaccgtgtg
    33901 gtgatcctct ccgccggtac aggtaacccg ttctttacca ccgactcagc agcttgcctg
    33961 cgtggtatcg aaattgaagc cgatgtggtg ctgaaagcaa ccaaagttga cggcgtgttt
    34021 accgctgatc cggcgaaaga tccaaccgca accatgtacg agcaactgac ttacagcgaa
    34081 gtgctcgaaa aagagctgaa agtcatggac ctggcggcct tcacgctggc tcgtgaccat
    34141 aaattaccga ttcgtgtttt caacatgaac aaaccgggtg cgctgcgccg tgtggtaatg
    34201 ggtgaaaaag aagggacttt aatcacggaa taattcccgt gatggataaa taagggtaag
    34261 attccgcgta agtatcgcgg gggcgtaagt ctggttataa ggcgttattg ttgcaggcag
    34321 tttggtcacg gccagcgcgc agcaaccgga gcgtacacaa gtgcgtgagg atggcgagca
    34381 ctgcccgggg ccaaaatggc aaataaaata gcctaataat ccagacgatt acccgtaata
    34441 tgtttaatca gggctatact tagcacactt ccactgtgtg tgactgtctg gtctgactga
    34501 gacaagtttt caaggattcg taacgtgatt agcgatatca gaaaagatgc tgaagtacgc
    34561 atggacaaat gcgtagaagc gttcaaaacc caaatcagca aaatacgcac gggtcgtgct
    34621 tctcccagcc tgctggatgg cattgtcgtg gaatattacg gcacgccgac gccgctgcgt
    34681 cagctggcaa gcgtaacggt agaagattcc cgtactctga aaatcaacgt gtttgatcgt
    34741 tcaatgtctc cggccgttga aaaagcgatt atggcgtccg atcttggcct gaacccgaac
    34801 tctgcgggta gcgacatccg tgttccgctg ccgccgctga cggaagaacg tcgtaaagat
    34861 ctgaccaaaa tcgttcgtgg tgaagcagaa caagcgcgtg ttgcagtacg taacgtgcgt
    34921 cgtgacgcga acgacaaagt gaaagcactg ttgaaagata aagagatcag cgaagacgac
    34981 gatcgccgtt ctcaggacga tgtacagaaa ctgactgatg ctgcaatcaa gaaaattgaa
    35041 gcggcgctgg cagacaaaga agcagaactg atgcagttct gatttcttga acgacaaaaa
    35101 cgccgctcag tagatccttg cggatcggct ggcggcgttt tgctttttat tctgtctcaa
    35161 ctctggatgt ttcatgaagc aactcaccat tctgggctcg accggctcga ttggttgcag
    35221 cacgctggac gtggtgcgcc ataatcccga acacttccgc gtagttgcgc tggtggcagg
    35281 caaaaatgtc actcgcatgg tagaacagtg cctggaattc tctccccgct atgccgtaat
    35341 ggacgatgaa gcgagtgcga aacttcttaa aacgatgcta cagcaacagg gtagccgcac
    35401 cgaagtctta agtgggcaac aagccgcttg cgatatggca gcgcttgagg atgttgatca
    35461 ggtgatggca gccattgttg gcgctgctgg gctgttacct acgcttgctg cgatccgcgc
    35521 gggtaaaacc attttgctgg ccaataaaga atcactggtt acctgcggac gtctgtttat
    35581 ggacgccgta aagcagagca aagcgcaatt gttaccggtc gatagcgaac ataacgccat
    35641 ttttcagagt ttaccgcaac ctatccagca taatctggga tacgctgacc ttgagcaaaa
    35701 tggcgtggtg tccattttac ttaccgggtc tggtggccct ttccgtgaga cgccattgcg
    35761 cgatttggca acaatgacgc ccgatcaagc ctgccgtcat ccgaactggt cgatgggacg
    35821 taaaatttcc gtcgattcgg ctaccatgat gaacaaaggt ctggaataca ttgaagcgcg
    35881 ttggctgttt aacgccagcg ccagccagat ggaagtgctg attcacccgc agtcagtgat
    35941 tcactcaatg gtgcgctacc aggacggcag tgttctggcg cagctgggtg aaccggatat
    36001 gcgtacgcca attgctcaca ccatggcatg gccgaatcgc gtgaactctg gcgtgaagcc
    36061 actcgatttt tgcaaactaa gtgcgttgac atttgccgca ccggattatg atcgttatcc
    36121 atgcctgaaa ctggcgatgg aggcgttcga acaaggccag gcagcgacga cagcattgaa
    36181 tgccgcaaac gaaatcaccg ttgctgcttt tcttgcgcaa caaatccgct ttacggatat
    36241 cgccgcgttg aatttatccg tactggaaaa aatggatatg cgcgaaccac aatgtgtgga
    36301 cgatgtgtta tctgttgatg cgaacgcgcg tgaagtcgcc agaaaagagg tgatgcgtct
    36361 cgcaagctga ggataatccg gctacagaga gtcgcgctat ttgttagcgt agggcttcag
    36421 tgatatagtc tgcgccatct gatcgtaagt agttggcttt ataaggtcag gtatgccgtg
    36481 gttttacacg gctttttttt gtataggctt cagtattcct gagtaccgta aaccctgtca
    36541 gggaataaga aacgcgtgat gttgtctgct actcaaccac ttagcgaaaa attgccagcg
    36601 catggctgtc gtcatgttgc gatcattatg gacggcaatg gtcgctgggc aaaaaagcaa
    36661 gggaagattc gtgcctttgg gcataaagcc ggggcaaaat ccgtccgccg ggctgtctct
    36721 tttgcggcca acaacggtat tgaggcgtta actctgtatg cctttagtag tgaaaactgg
    36781 aaccgaccag cgcaggaagt cagtgcgtta atggaactgt ttgtgtgggc gcttgatagc
    36841 gaagtaaaga gtctgcaccg acataacgtg cgtctgcgta ttattggcga taccagtcgc
    36901 tttaactcgc gtttgcaaga acgtattcgt aaatctgaag cgctaacagc cgggaatacc
    36961 ggtctgacgc tgaatattgc ggcgaactac ggtggacgtt gggatatagt ccagggagtc
    37021 aggcaactgg ctgaaaaggt gcagcaagga aacctgcaac cagatcagat agatgaagag
    37081 atgctaaacc agcatgtctg tatgcatgaa ctggcccctg tagatttagt aattaggact
    37141 gggggggagc atcgcattag taactttttg ctttggcaaa ttgcctatgc cgaactttac
    37201 tttacagatg ttctctggcc cgatttcgat gaacaagact ttgaaggggc gttaaatgcc
    37261 tttgctaatc gagagcgtcg tttcggcggc accgagcccg gtgatgaaac agcctgatgg
    37321 gggtcgcttt tgctgaagta tcgcctgata tctgcttttg tgttaatacc cgtcgtcatc
    37381 gcggcgttgt ttctgttgcc gccggtgggg ttcgccattg taacgctggt ggtctgcatg
    37441 ctggcagcgt gggaatgggg acagcttagc ggttttacca ctcgctcgca gcgagtatgg
    37501 ttggcggtgt tatgtgggtt attgttggcg ctgatgcttt ttctgttgcc ggaatatcac
    37561 cgaaatattc atcaaccgct ggttgaaatc tcactttggg cttcgctggg ttggtggagt
    37621 gtcgcgctat tgctggtgct gttttaccca ggttccgcag caatctggcg taactctaaa
    37681 acattgcgca ttatttttgg cgtgctaacc attgttccct tcttctgggg catgctggcg
    37741 ttacgggcct ggcactacga cgagaatcat tacagtggcg caatatggct gctctatgtc
    37801 atgatcctgg tatggggcgc tgactctggc gcatatatgt ttggcaaatt gtttggtaaa
    37861 cataaactgg caccgaaggt ttctccgggt aaaacctggc aaggctttat aggtggactc
    37921 gcgactgcag cggtaatctc atggggttat ggcatgtggg cgaatctcga cgtcgctccc
    37981 gtcaccttac tcatttgctc tattgtcgca gcgttagcct cagtgctcgg cgatctgacc
    38041 gagagtatgt ttaagcgtga agcaggaatt aaggacagcg gtcatttaat tccaggacac
    38101 ggtggtattt tagatcgtat tgatagcctg acggctgcgg taccggtctt tgcttgcttg
    38161 ttgttactgg tattcaggac gctttaacgg aaggtaatat gctgagtttt ctctgggatt
    38221 tggcttcgtt catcgttgca ctgggtgtac ttatcaccgt gcatgaattt ggtcatttct
    38281 gggttgcccg gcgttgtggt gttcgcgttg agcgtttctc aatagggttt ggtaaggcgc
    38341 tctggcggcg aactgataag ctcggcaccg aatatgttat cgccctgatc ccgctgggcg
    38401 gttatgtcaa aatgctggat gagcgcgcag aaccggtcgt tccggaactc cgccaccatg
    38461 ccttcaataa taaatctgtc ggccaacgag cggcgattat tgccgcaggt ccggttgcaa
    38521 acttcatttt tgctatcttt gcctactggc tggtttttat tattggtgtg cctggcgtac
    38581 gtccggtggt tggcgaaata gcagccaatt cgatagctgc agaagcacaa attgcaccag
    38641 gtacggaact aaaagccgta gatggtatcg aaacgcctga ttgggatgcc gtgcgtttgc
    38701 agttggtcga taaaattggc gatgaaagca ccaccattac agtagcgcca tttggcagcg
    38761 accaacggcg ggatgtaaag ctcgatttac gtcactggac gtttgagcct gataaagaag
    38821 atccgataac ttcgctgggg attcgtcctc gtgggccgca aattgaatct gtactggaaa
    38881 atgtgcagcc aaactcggcg gcaagcaagg caggtttgca agcaggcgac aggatcgtta
    38941 aagtcgatgg tcagccctta acgcagtggg tgacctttgt gatgcttgtc cgggataacc
    39001 cgggtaaatc cttagcgtta gaaatcgaaa ggcaggggag tcccttgtct ttgacattaa
    39061 tcccggagag taaaccgggt aatggtaaag cgattggttt tgtcggtatt gagccgaaag
    39121 tcattccttt gccagatgag tataaagttg tacgccagta tgggccgttc aacgccatcg
    39181 tcgaagccac ggacaaaacg tggcagctga tgaagctgac ggtcagtatg ctgggaaaat
    39241 tgatcaccgg tgatgtgaaa ctgaacaacc tcagtgggcc gatctctatc gccaaggggg
    39301 ctgggatgac agcggaactc ggggttgttt attacctgcc gtttcttgcg cttattagcg
    39361 tgaacttagg gataattaac ctgtttccgt tgcccgtact tgacgggggg catctgctgt
    39421 tccttgcgat cgaaaagatc aagggcggac cggtatccga gcgggttcaa gacttttgtt
    39481 atcgcattgg ctcgattctg ctggtgctgt taatggggct tgcacttttc aatgatttct
    39541 ctcggttatg agagttagtt aggaagaacg cataataacg atggcgatga aaaagttgct
    39601 catagcgtcg ctgctgttta gcagcgccac cgtatacggt gctgaagggt tcgtagtgaa
    39661 agatattcat ttcgaaggcc ttcagcgtgt cgccgttggt gcggccctcc tcagtatgcc
    39721 ggtgcgcaca ggcgacacgg ttaatgatga agatatcagt aataccattc gcgctctgtt
    39781 tgctaccggc aactttgagg atgttcgcgt ccttcgtgat ggtgataccc ttctggttca
    39841 ggtaaaagaa cgtccgacca ttgccagcat tactttctcc ggtaacaaat cggtgaaaga
    39901 tgacatgctg aaacaaaacc tcgaggcttc tggtgtgcgt gtgggcgaat ccctcgatcg
    39961 caccaccatt gccgatatcg agaaaggtct ggaagacttc tactacagcg tcggtaaata
    40021 tagcgccagc gttaaagctg tcgtgacccc gctgccgcgc aaccgtgttg acctaaaact
    40081 ggtgttccag gaaggtgtgt cagctgaaat ccagcaaatt aacattgttg gtaaccatgc
    40141 tttcaccacc gacgaactga tctctcattt ccaactgcgt gacgaagtgc cgtggtggaa
    40201 cgtggtaggc gatcgtaaat accagaaaca gaaactggcg ggcgaccttg aaaccctgcg
    40261 cagctactat ctggaccgcg gttatgcccg tttcaacatc gactctaccc aggtcagtct
    40321 gacgccagat aaaaaaggta tttacgtcac ggtgaacatc accgaaggcg atcagtacaa
    40381 gctttctggc gttgaagtga gcggcaacct tgccgggcac tccgctgaaa ttgagcagct
    40441 gactaagatc gagccgggcg agctgtataa cggcaccaaa gtgaccaaaa tggaagatga
    40501 cattaaaaag cttctcggtc gctatggtta tgcctatccg cgcgtacagt cgatgcctga
    40561 aattaacgat gccgacaaaa ccgttaaatt acgcgtgaac gttgatgcgg gtaaccgttt
    40621 ctacgtgcgt aagatccgtt ttgaaggtaa cgatacctcg aaagatgccg tcctgcgtcg
    40681 cgaaatgcgt cagatggaag gtgcatggct ggggagcgat ctggtcgatc agggtaagga
    40741 gcgtctgaat cgtctgggct tctttgaaac tgtcgatacc gatacccaac gtgttccggg
    40801 tagcccggac caggttgatg tagtctacaa ggtgaaagaa cgtaacaccg gtagcttcaa
    40861 cttcgggatt ggttacggta ctgaaagtgg cgtgagcttc caggctggtg tgcaacagga
    40921 taactggtta ggtacgggtt atgctgttgg tatcaacggg accaaaaacg attaccagac
    40981 ctatgctgaa ctgtcggtaa ccaacccgta cttcaccgta gatggcgtaa gcctcggtgg
    41041 tcgtctcttc tataatgact tccaggcaga tgacgccgac ctgtccgact ataccaacaa
    41101 gagttatggt acagacgtga cgttgggctt cccgatcaac gaatataact cgctgcgtgc
    41161 aggtctgggt tatgtacata actccctgtc caacatgcag cctcaggttg cgatgtggcg
    41221 ttatctgtac tctatgggtg aacatccgag cacctctgat caggataaca gcttcaaaac
    41281 ggacgacttc acgttcaact atggttggac ctataacaag cttgaccgtg gttacttccc
    41341 gacagatggt tcacgtgtca acctgactgg taaagtgacc attccgggat cggataacga
    41401 atactacaaa gtgacgttag acacggcgac ttatgtgccg atcgatgacg atcacaaatg
    41461 ggttgttctg ggtcgtaccc gctggggtta tggtgatggt ttaggcggca aagagatgcc
    41521 gttctacgag aacttctatg ccggtggttc cagcaccgtc cgtggcttcc agtccaatac
    41581 cattggtccg aaagcagttt acttcccgca tcaggctagt aattatgatc cggactatga
    41641 ttacgaatgt gcgactcagg acggcgcgaa agacctgtgt aaatcggatg atgctgtagg
    41701 cggtaacgct atggcggttg ccagcctcga gttcatcacc ccgacgccgt ttattagcga
    41761 taagtatgct aactcggttc gtacttcctt cttctgggat atgggtaccg tttgggatac
    41821 aaactgggat tccagccaat attctggata tccggactat agtgatccaa gcaatatccg
    41881 tatgtctgcg ggtatcgcat tacaatggat gtccccattg gggccgttgg tgttctccta
    41941 cgcccagccg ttcaaaaagt acgatggaga caaggcagaa cagttccagt ttaacatcgg
    42001 taaaacctgg taagtgttct ccacaaagga atgtagtggt agtgtagcga tgactttagg
    42061 cgatcaatat aagatcgccg ggccacgcaa agaactgcac cctccggtgc aaatgggatg
    42121 gtaaggagtt tattgtgaaa aagtggttat tagctgcagg tcttggttta gcactggcaa
    42181 cttctgctca ggcggctgac aaaattgcaa tcgtcaatat gggcagcctg ttccagcagg
    42241 tagcgcagaa aaccggtgtt tctaacacgc tggaaaatga gttcaaaggc cgtgccagcg
    42301 aactgcagcg tatggaaacc gatctgcagg ctaaaatgaa aaagctgcag tccatgaaag
    42361 cgggcagcga tcgcaccaaa ctggaaaaag acgtgatggc tcagcgccag acttttgctc
    42421 agaaagcgca ggcttttgag caggatcgcg cacgtcgttc caacgaagaa cgcggcaaac
    42481 tggttactcg tatccagact gctgtgaaat ccgttgccaa cagccaggat atcgatctgg
    42541 ttgttgatgc aaacgccgtt gcttacaaca gcagcgatgt aaaagacatc actgccgacg
    42601 tactgaaaca ggttaaataa gtaatgcctt caattcgact ggctgattta gcgcagcagt
    42661 tggatgcaga actacacggt gatggcgata tcgtcatcac cggcgttgcg tccatgcaat
    42721 ctgcacaaac aggtcacatt acgttcatgg ttaacccaaa ataccgtgag catttaggct
    42781 tgtgccaggc gtccgcggtt gtcatgaccc aggacgatct tcctttcgcg aaaagtgccg
    42841 cgctggtagt gaagaatccc tacctgactt acgcgcgcat ggcgcaaatt ttagatacca
    42901 cgccgcagcc cgcgcagaac attgcaccca gtgcggtgat cgacgcgacg gcgaagctgg
    42961 gtaacaacgt atcgattggc gctaacgcgg tgattgagtc cggcgttgaa ctgggcgata
    43021 acgtgattat cggtgccggt tgcttcgtag gtaaaaacag cagaatcggt gcaggttcgc
    43081 gtctctgggc gaacgtaacc atttaccatg agatccagat cggtcagaat tgcctgatcc
    43141 agtccggaac agtggtaggt gcagacggct ttggttatgc caacgatcgt ggtaactggg
    43201 tgaagatccc acagattggt cgcgtaatta ttggcgatcg cgtggagatc ggcgcatgta
    43261 ctaccatcga tcgcggcgcg ctggatgaca ctgttattgg caatggcgtg atcattgata
    43321 accagtgcca gattgcacat aacgtcgtga ttggcgacaa tacggcggtt gccggtggcg
    43381 tcattatggc gggcagcctg aaaattggtc gttactgcat gatcggcgga gccagcgtaa
    43441 tcaacgggca tatggaaata tgcgacaaag tgacggttac gggcatgggt atggtgatgc
    43501 gtcccatcac tgaaccaggc gtctattcct caggcattcc gctgcaaccc aacaaagtct
    43561 ggcgcaaaac cgctgcactg gtgatgaaca ttgatgacat gagcaagcgt ctgaaatcgc
    43621 ttgagcgcaa ggttaatcaa caagactaac gttccatctt ttgttcgcca aactttacgg
    43681 cctgtctcat tcttacgatt gcggcaggcc gtgttattat tgtcgtttct tatattttga
    43741 caggaagagt atcttgacta ctaacactca tactctgcag attgaagaga ttttagaact
    43801 tctgccgcac cgtttcccgt tcttactggt ggatcgcgtg ctggattttg aagaaggtcg
    43861 ttttctgcgc gcagtaaaaa atgtctctgt caatgagcca ttcttccagg gccatttccc
    43921 tggaaaaccg attttcccgg gtgtgctgat tctggaagca atggcacagg caacaggtat
    43981 tctggcgttt aaaagcgtag gaaaactgga accgggtgag ctgtactact tcgctggtat
    44041 tgacgaagcg cgcttcaagc gcccggtcgt gcctggcgat caaatgatca tggaagtcac
    44101 tttcgaaaaa acgcgccgcg gcctgacccg ttttaaaggg gttgctctgg tcgacggtaa
    44161 agtagtttgc gaagcaacga tgatgtgtgc tcgtagccgg gaggcctgat acgtgattga
    44221 taaatccgcc tttgtgcatc caaccgccat tgtggaagag ggcgcgtcga ttggcgctaa
    44281 cgctcacatt ggtccttttt gtatcgttgg accccatgtc gaaattggtg agggtaccgt
    44341 actgaaatct cacgttgtcg tgaatggtca tactaaaatt ggccgcgata atgagattta
    44401 tcagttcgcc tccatcggcg aagttaacca ggatctgaaa tatgctggcg aaccgacccg
    44461 cgtggaaatc ggcgatcgta accgcattcg cgaaagcgtc accattcatc gtggcacagt
    44521 ccagggcggt ggattgacga aggtgggcag cgacaactta ctgatgatca acgcgcacat
    44581 tgcgcacgat tgtacggtag gtaaccgctg tattctcgcc aacaacgcaa cgctggcggg
    44641 tcacgtatcg gttgacgact tcgcgatcat cggcggcatg accgcagtcc atcagttctg
    44701 catcattggt gcgcatgtga tggttggcgg ctgctccggt gtggcgcagg acgtccctcc
    44761 ttatgtcatt gcgcagggta accacgcaac gccgttcggt gtcaatatcg aagggctgaa
    44821 gcgccgcgga ttcagccgtg aggcgattac cgctatccgc aatgcgtata agctgattta
    44881 tcgtagcggt aaaacgctcg atgaagtgaa accggaaatt gctgaactgg cggaaacata
    44941 tccggaagtg aaagccttta ccgatttctt tgcacgctca acgcgcggtc tgattcgtta
    45001 atgactgaac agcgtccatt aacgattgcc ctggtcgccg gagaaacctc cggcgatatc
    45061 ctgggggccg gtttaatccg cgctctgaaa gaacgtgtgc ccaacgcccg ctttgttggt
    45121 gttgccgggc cacgaatgca ggctgaaggc tgcgaagcct ggtacgaaat ggaagaactg
    45181 gcggtgatgg gcattgttga agtgctcggt cgtctgcgtc gcttactgca tattcgtgcc
    45241 gatctgacaa agcgttttgg cgaactgaag ccagatgttt ttgttggtat tgatgcgccc
    45301 gacttcaata ttactcttga aggtaacctc aaaaagcagg gtatcaaaac cattcattac
    45361 gtcagtccat ccgtctgggc gtggcgacag aaacgcgttt tcaaaatagg cagagccacc
    45421 gatctggtgc tcgcatttct gcctttcgaa aaagcgtttt atgacaaata caatgtaccg
    45481 tgccgcttta tcggtcatac catggctgat gccatgccat tagatccaga taaaaatggt
    45541 gcccgtgatg tgctggggat cccttacgat gcccactgtc tggctttgtt gccgggcagc
    45601 cgtggtgcag aagtcgaaat gcttagtgct gatttcctga aaacggctca gcttttgcgc
    45661 cagacatatc cggatctcga aatcgtggtg ccgctggtga atgccaaacg ccgcgagcag
    45721 tttgaacgca tcaaagctga agttgcgcca gacctgtcgg ttcatatgtt ggatgggatg
    45781 ggccgtgagg cgatggtcgc tagtgatgcg gcactactgg cgtcggggac ggcagccctg
    45841 gagtgcatgc tggcgaaatg cccgatggtg gtggggtatc gcatgaagcc tttcactttc
    45901 tggttggcga agcgtctggt gaaaactgat tatgtctcgc tgccaaattt gctggcgggc
    45961 agagagttag tcaaagagtt attgcaggaa gagtgtgagc cgcaaaaact ggctgcggcg
    46021 ctgttaccgc tgttggcgaa cgggaaaacc agccacgcga tgcacgatac cttccgtgaa
    46081 ctgcatcagc agatccgctg caatgccgat gagcaggcgg cacaagccgt tctggagtta
    46141 gcacaatgat cgaatttgtt tatccgcaca cgcagctggt tgcgggtgtg gatgaagtcg
    46201 gacgcgggcc gttagttggc gcggtcgtca ccgctgcggt gatccttgac ccatcgcgcc
    46261 cgattgccgg gctgaatgat tccaaaaagc tgagcgaaaa acgccgtctg gcgctctgtg
    46321 aagagatcaa agataaagcg ttgagctgga gtctgggccg cgcggaaccc cacgaaatcg
    46381 acgaactgaa tattttacac gcgaccatgc tggcgatgca gcgtgccgtc gctgggctgc
    46441 atattgcgcc ggaatatgtg ttgattgatg gtaaccgctg cccgaaatta ccgatgcctt
    46501 cgatggcagt ggtgaaaggc gatagccgcg taccagaaat cagtgctgcg tctatcctgg
    46561 cgaaagtgac gcgcgacgcc gaaatggcgg cgctggatat tgttttcccg caatatggtt
    46621 ttgcccaaca caaagggtat ccaactgctt ttcatctgga aaaactggct gaatatggcg
    46681 cgactgaaca tcatcgttgc agctttgggc ctgtcaaacg cgcactggga cttgcgtcct
    46741 gattcttgtg tcgagattaa gtaaaccgga atctgaagat gtctgaacca cgtttcgtac
    46801 acctgcgggt gcacagcgac tactcgatga tcgatggtct ggccaaaacc gcaccgctgg
    46861 taaaaaaggc ggcggcgttg ggtatgccag cactggcgat caccgatttc accaaccttt
    46921 gcggtctggt gaagttctac ggagcgggac atggcgcagg gattaagcct atcgtcgggg
    46981 cagattttaa tgtccagtgc gacctgctgg gtgatgagtt aacccacctg acggtgctgg
    47041 cggcgaacaa taccggctat cagaatctga cgttgctgat ctcaaaagcg tatcagcgcg
    47101 ggtacggtgc agctgggccg atcatcgatc gcgactggct tatcgaatta aacgaagggt
    47161 tgatccttct ttccggcggg cgcatgggcg acgtcggacg cagtcttttg cgtggtaaca
    47221 gcgcgctggt agatgagtgt gtcgcgtttt atgaagaaca cttcccggat cgctattttc
    47281 tcgagctgat ccgcaccggc aggccggatg aagaaagcta tctgcacgcg gcggtggaac
    47341 tggcggaagc gcgcggtttg cccgtcgtgg cgactaacga tgtgcgcttt atcgacagca
    47401 gcgactttga cgcacacgaa atccgcgtcg cgatccacga cggctttacc ctcgacgatc
    47461 ctaaacgccc gcgtaactat tcgccgcagc aatatatgcg tagcgaagag gagatgtgcg
    47521 agctgtttgc cgacatccca gaagcccttg ccaacaccgt tgagatcgcc aaacgctgta
    47581 acgtaactgt gcgtcttggt gaatacttcc tgccgcagtt cccgaccggg gacatgagca
    47641 ccgaagatta tctggtcatg cgtgcaaaag agggcctgga agagcgtctg gcatttttat
    47701 tccctgacga ggaagaacgt gttaagcgcc gcccggaata tgacgaacgt ctggagactg
    47761 aacttcaggt tatcaaccag atgggcttcc cgggctactt cctcatcgtt atggaattta
    47821 tccagtggtc gaaagataac ggcgtaccgg tagggccagg ccgtggctcc ggtgcgggtt
    47881 cactggtggc ctacgcgctg aaaatcaccg acctcgatcc gctggaattt gacctgctgt
    47941 tcgaacgttt ccttaacccg gaacgtgtct ccatgcctga cttcgacgtt gacttctgta
    48001 tggagaaacg cgaccaggtt atcgagcacg tagcggacat gtacggtcgt gatgcggtat
    48061 cgcagattat caccttcggt acgatggcgg cgaaagcggt gatccgcgac gtaggccgcg
    48121 tgctggggca tccgtacggc tttgtcgacc gtatctcgaa actgatcccg cccgatccgg
    48181 ggatgacgct ggcgaaagcg tttgaagccg agccgcagct gccggaaatc tacgaagcgg
    48241 atgaagaagt taaggcgctg atcgacatgg cgcgcaaact ggaaggggtc acccgtaacg
    48301 ccggtaagca cgccggtggg gtggttatcg caccgaccaa aattaccgat tttgcgccgc
    48361 tttactgcga tgaagagggc aaacatccgg tcacccagtt tgataaaagc gacgttgaat
    48421 acgccggact ggtgaagttc gacttccttg gtttgcgtac gctcaccatc atcaactggg
    48481 cgctggagat gatcaacaag cggcgggcga agaatggcga gccgcctctg gatatcgccg
    48541 cgatcccgct ggatgacaag aaaagcttcg acatgctgca acgctcggaa accactgcgg
    48601 tattccagct tgaatcgcgc ggcatgaagg acctgatcaa gcgtctgcaa cctgactgct
    48661 tcgaagatat gatcgcactg gtggcactgt tccgccccgg tccgttgcaa tcagggatgg
    48721 tggataactt tatcgaccgt aaacatggtc gcgaagagat ctcctatccg gacgtgcagt
    48781 ggcagcatga aagcctgaaa ccggtactgg agccaaccta cggcatcatc ctgtatcagg
    48841 aacaggtcat gcagattgcc caggtgcttt ctggttatac cctcggtggc gcggatatgc
    48901 tgcgtcgtgc gatgggtaag aaaaagccgg aagagatggc taagcagcgt tctgtatttg
    48961 ctgaaggtgc agaaaagaac ggaatcaacg ccgaactggc gatgaaaatc ttcgacctgg
    49021 tggagaaatt cgctggttac ggatttaaca aatcgcactc tgcggcctat gctttggtgt
    49081 catatcaaac gttatggctg aaagcgcact atccggcgga gtttatggcg gcggtaatga
    49141 ccgccgatat ggacaacacc gagaaggtgg tgggcctggt ggatgagtgc tggcggatgg
    49201 ggctgaaaat cctgccgccg gatataaact ccggtcttta ccatttccac gtcaacgacg
    49261 acggcgaaat cgtgtatggt attggcgcga tcaaaggggt cggtgaaggt ccgattgagg
    49321 cgatcatcga agcccgtaat aaaggcggct acttccgcga actgtttgat ctctgcgccc
    49381 gaaccgacac caaaaagtta aaccggcggg tgctggaaaa actgatcatg tccggggcgt
    49441 ttgaccgtct tgggccacac cgcgcggcgc tgatgaactc gctgggcgat gcgttaaaag
    49501 cggcagatca acacgcgaaa gcggaagcta tcggtcaggc cgatatgttc ggtgtgctgg
    49561 ccgaagagcc ggaacaaatt gaacaatcct acgccagctg ccaaccgtgg ccggagcagg
    49621 tggtattaga tggggaacgt gaaacgttag gtctgtacct gacgggacac cctatcaatc
    49681 agtatttaaa agagattgag cgttatgtcg gaggcgtaag gctgaaagac atgcacccga
    49741 cagaacgtgg taaagtcatc acggctgcgg ggctcgttgt tgccgcgcgg gttatggtca
    49801 ccaagcgcgg caatcgtatc ggtatctgca cgctggatga ccgttccggg cggctggaag
    49861 tgatgttgtt tactgacgcc ctggataaat accagcaatt gctggaaaaa gaccgcatac
    49921 ttatcgtcag cggacaggtc agctttgatg acttcagcgg tgggcttaaa atgaccgctc
    49981 gcgaagtgat ggatattgac gaagcccggg aaaaatatgc tcgcgggctt gctatctcgc
    50041 tgacggacag gcaaattgat gaccagcttt taaaccgact ccgtcagtct ctggaacccc
    50101 accgctctgg gacaattcca gtacatctct actatcagag ggcggatgca cgcgcgcggt
    50161 tgcgttttgg cgcgacgtgg cgtgtctctc cgagcgatcg tttattaaac gatctccgtg
    50221 gcctcattgg ttcggagcag gtggaactgg agtttgacta atacaggaat actatgagtc
    50281 tgaatttcct tgattttgaa cagccgattg cagagctgga agcgaaaatc gattctctga
    50341 ctgcggttag ccgtcaggat gagaaactgg atattaacat cgatgaagaa gtgcatcgtc
    50401 tgcgtgaaaa aagcgtagaa ctgacacgta aaatcttcgc cgatctcggt gcatggcaga
    50461 ttgcgcaact ggcacgccat ccacagcgtc cttataccct ggattacgtt cgcctggcat
    50521 ttgatgaatt tgacgaactg gctggcgacc gcgcgtatgc agacgataaa gctatcgtcg
    50581 gtggtatcgc ccgtctcgat ggtcgtccgg tgatgatcat tggtcatcaa aaaggtcgtg
    50641 aaaccaaaga aaaaattcgc cgtaactttg gtatgccagc gccagaaggt taccgcaaag
    50701 cactgcgtct gatgcaaatg gctgaacgct ttaagatgcc aatcatcacc tttatcgaca
    50761 ccccgggggc ttatccgggc gtgggcgcag aagagcgcgg tcagtctgaa gccattgcac
    50821 gcaacctgcg tgaaatgtct cgccttagcg taccggtagt ttgtacggtt atcggtgaag
    50881 gtggttctgg cggtgcgctg gcgattggcg tgggcgataa agtgaatatg ctgcaataca
    50941 gcacctattc cgttatctcg ccggaaggtt gtgcgtccat tctgtggaag agcgccgaca
    51001 aagcgccgct ggcggctgaa gcgatgggta tcatcgctcc gcgtctgaaa gagctgaaac
    51061 tgatcgactc catcatcccg gaaccgctgg gtggtgctca ccgtaacccg gaagcgatgg
    51121 cggcgtctct gaaagtgcag ctgctggcgg atctggccga tctcgacgtg ttaagcactg
    51181 aagatttaaa aaatcgtcgt tatcagcgcc tgatgagcta cggttacgcg taattcgcaa
    51241 atgttctgaa aagggtcact tcggtggccc ttttttatcg ccacggtttg agcaggctat
    51301 gattaaggaa ggattttcca ggaggaacac atgaacatca ttgccattat gggaccgcat
    51361 ggcgtctttt ataaagatga gcccatcaaa gaactggagt cggcgctggt ggcgcaaggc
    51421 tttcagatta tctggccaca aaacagcgtt gatttgctga aatttattga acataaccct
    51481 cgaatttgcg gcgtgatttt tgactgggat gagtacagtc tcgatttatg tagcgatatc
    51541 aatcagctta atgaatatct cccgctttat gccttcatca acacccactc gacgatggat
    51601 gtcagcgtgc aggatatgcg gatggcgctc tggttttttg aatatgcgct ggggcaggca
    51661 gaagatatcg ccattcgtat gcgtcagtac accgacgaat atcttgataa cattacaccg
    51721 ccgttcacga aagccttgtt tacctacgtc aaagagcgga agtacacctt ttgtacgccg
    51781 ggtcatatgg gcggcaccgc atatcaaaaa agcccggttg gctgtctgtt ttatgatttt
    51841 ttcggcggga atactcttaa ggctgatgtc tctatttcgg ttaccgagct tggttcgttg
    51901 ctcgaccaca ccgggccaca cctggaagcg gaagagtaca tcgcgcggac ttttggcgcg
    51961 gaacagagtt atatcgttac caacggaaca tcgacgtcta acaaaattgt gggtatgtac
    52021 gccgcgccgt ccggcagtac gctgttgatc gaccgcaatt gtcataaatc gctggcgcat
    52081 ctgttgatga tgaacgatgt agtgccagtc tggctgaaac cgacgcgtaa tgcgttgggg
    52141 attcttggtg ggatcccgcg ccgtgaattt actcgcgaca gcatcgaaga gaaagtcgct
    52201 gctaccacgc aagctcaatg gccggttcat gcggtgatca ccaactccac ctatgatggc
    52261 ttgctctaca acaccgactg gatcaaacag acgctggatg tcccgtcgat tcacttcgat
    52321 tctgcctggg tgccgtacac ccattttcat ccgatctacc agggtaaaag tggtatgagc
    52381 ggcgagcgtg ttgcgggaaa agtgatcttc gaaacgcagt cgacccacaa aatgctggcg
    52441 gcgttatcgc aggcttcgct gatccacatt aaaggcgagt atgacgaaga ggcgtttaac
    52501 gaagccttta tgatgcatac caccacctcg cccagttatc ccattgttgc ttcggttgag
    52561 acggcggcgg cgatgctgcg tggtaatccg ggcaaacggc tgattaaccg ttcggtggag
    52621 cgggcgctgc attttcgcaa agaggtccag cggctgcggg aagagtctga cggttggttt
    52681 ttcgatatct ggcaaccgcc gcaggtggat gaagccgaat gctggcccgt tgcgcctggc
    52741 gaacagtggc acggctttaa cgatgcggat gccgatcata tgtttctcga tccggttaaa
    52801 gtcactattt tgacaccggg gatggacgag cagggcaata tgagcgagga ggggatcccg
    52861 gcggcgctgg tagcaaaatt cctcgacgaa cgagggatcg tagtagagaa aaccggtcct
    52921 tataacctgc tgtttctctt tagtattgga atcgataaaa ccaaagcaat gggattattg
    52981 cgtgggttga cggaatttaa gcgctcttac gatctcaacc tgcggatcaa aaatatgcta
    53041 cccgatctct atgcagaaga tcccgatttc taccgcaata tgcgcattca ggatctggcg
    53101 caagggatcc ataagctgat tcgtaaacac gatcttcccg gtttgatgtt gcgggcattc
    53161 gataccttgc cggagatgat catgacgcca catcaggcat ggcagaggca gattaaaggc
    53221 gaagtagaaa ccattgcgct ggaacaactg gttggtaggg tgtcggcaaa tatgatcctg
    53281 ccttatccac cgggcgtacc gctgctgatg ccaggagaaa tgctgaccaa agagagccgc
    53341 acagtactcg attttctact gatgctttgt tccgtagggc aacattaccc cggttttgaa
    53401 acggatattc acggcgcgaa acaggacgaa gacggcgttt accgcgtacg agtcctaaaa
    53461 atggcgggat aacttgccag agcggcttcc gggcgagtaa cgtgctgtta acaaataaag
    53521 gagacgttat gctgggttta aaacaggttc accatattgc gattattgcg acggattatg
    53581 cggtgagcaa agcgttttac tgcgatattc ttggtttcac gctgcaaagc gaagtctatc
    53641 gcgaagcgcg cgactcgtgg aaaggggatt tggcgcttaa tgggcaatat gtgattgagc
    53701 ttttctcatt tccgttcccg ccggagcgcc ccagccgacc ggaagcttgc ggcctgcgtc
    53761 atctggcttt tagcgttgat gatatcgatg cggcagtggc gcaccttgaa agccataacg
    53821 tgaagtgtga agccatccgt gtcgatccat acacgcaaaa acgcttcacg ttctttaacg
    53881 atccggacgg gctgccgttg gaactgtatg agcagtaagg cttgtcatcg ccgcatttgc
    53941 ccggtaacgt gccgggcatt gctactgtaa aatcgcacca tcatgacact cacgctcaat
    54001 agacaacttc tcacctcacg ccagattctg gtggccttta gcggcgggct tgactctacc
    54061 gtactgctgc atcagttggt gcagtggcgg gcggaaaatc cgggcgtcac tctgcgcgct
    54121 atccatgtgc atcacggttt aagtgccaat gccgatgcct gggttaggca ttgcgagaac
    54181 atctgccaac agtggcaggt gccgctggtg gtcgagcgcg tgcaacttgc gcaagaagga
    54241 ctgggtattg aggcccaggc gcggcaggcg cgttatcagg catttgcccg caccttgttg
    54301 cccggtgaag tgctggtcac cgcgcaacat ctcgacgatc aatgcgaaac ctttctgctg
    54361 gcgctaaaac gcggtagcgg ccctgccggg ctttcggcta tgggggaagt ttcggagttt
    54421 gccggaacac ggcttatccg cccgttgctc gcccgcacgc ggggagaact ggtgcagtgg
    54481 gcgcgtcagt atgatttacg ctggattgaa gacgaaagta atcaggatga tagctacgat
    54541 cgtaacttcc tgcgcctgcg cgtggtgccg ttattgcagc agcgttggcc gcattttgcc
    54601 gaagcaacgg cccgcagcgc tgcgctttgc gctgaacaag agagcctgtt agatgaactg
    54661 ctggcggatg atatagcgca ctgtcaaaag ccgcagggga cgctgcagat tgcgccaatg
    54721 ctggcgatga gtgatgcccg ccgcgcggcg attatccgcc gctggctggc agggaagaat
    54781 gcaccgatgc cttcccgcga cgcgttggtg aggatctggc aggaagtggc gctggcgcgg
    54841 gaagatgctt ccccctgttt acgtctgggc gcgtttgaaa tccgccgcta tcagtcgcaa
    54901 ctgtggtgga ttaaatccgt tgccgggcaa agcgaaacca ttgtgccgtg gcagacgtgg
    54961 ctacaaccgc tggaattacc ggcgggactg ggaagtgtac agcttacagc gggaggcgat
    55021 attcgccctc cgcgtgcaga cgaagcggtc agcgtgcgtt tcaaagcgcc aggattgctg
    55081 catattatcg ggcgcaacgg cggacgtaag ctgaagaaaa tctggcaaga gctgggtgtg
    55141 ccgccgtggc tacgtgacac cacgccactg ctgttttatg gcgaaacgtt gattgcggcg
    55201 gcaggggtat ttgtgacgca agaaggtgtg gctgaaggtg agaaaggcgt cagttttgtc
    55261 tggcagaaaa cgcttagtta agtgaaagcc ggataagacg cttcaaacgt cgcatccggc
    55321 gaaagtcaat caggactcgc tcaccaccac cgtaccgatt tccgggtggc taaagctggt
    55381 aattttatcc agacgcagct cgcgggttgc accagcggcc tcgacgacca gatactccac
    55441 atttttgcgg gagactaaat cgctggcctt cgcctgcaat ttttcgccat ctttcagctc
    55501 aagtgtcagc attaaatgat gctggcaggc gagctcaaga ttatcgtaat catcacaatt
    55561 gattggttga tacgtatcat tcattgacat aatcgctcac cagtaagttt gccgcagcgt
    55621 atgctgcttt ttccctgaca gcctcagaaa gggcgtcgtc ggcagccatt tcattcagca
    55681 ctttcaaaac gcagcccagc gcgtccggaa cgtatcctaa gtctccgctg gcgatttccg
    55741 cgtaccgctt gcgtattaac tcacaatatt tttccacatg ccctcctgtc agcactctga
    55801 cttaaccgtg gatgcaagtc taagcctacg aagataaact ctgtttcgca aggtgactat
    55861 accacactca tttctgcaat atcagcgccg caactgcacg tattccgtta caatggcctc
    55921 ctgattcaaa aggagttttc ttatggcgct taaagcgaca atttataaag cgacggttaa
    55981 tgtggccgat ctcgaccgca accagtttct cgatgcctct ctgacgctgg cgcgccaccc
    56041 ttcagaaacc caggagcgca tgatgctgcg cctgctggcg tggctgaaat atgctgatga
    56101 acgtctgcaa tttacccgtg gtttgtgtgc cgatgatgag ccggaagcgt ggctgcgtaa
    56161 cgatcacctg ggcattgatt tgtggattga gctggggctg ccggatgagc ggcggattaa
    56221 gaaagcctgc acccaggccg cagaagttgc gctgtttacc tataatagtc gggcggcgca
    56281 aatctggtgg cagcaaaatc agagcaaatg tgtgcagttt gccaatcttt ccatctggta
    56341 tctggacgat gaacaactgg cgaaagtaag cgcctttgcc gatcgtacca tgacgctgca
    56401 ggcaacgatt caggatggcg tgatctggtt atcggatgat aagaataatc tggaagtgaa
    56461 cttaaccgtc tggcaacaac cttcatgatt gtgatttccc gacatgttgc tatccccgat
    56521 ggtgagcttg agatcaccgc cattcgtgcg cagggcgcgg gcgggcagca tgttaataag
    56581 acctcaacgg ctattcatct gcgttttgac attcgggcgt ccagcctgcc agagtattac
    56641 aaagagcgtc tgctcgccgc cagccatcat ttgatcagca gtgatggcgt gattgtcatt
    56701 aaggcacagg aataccgcaa tcaggagctc aaccgcgaag cggcgctggc ccggctggta
    56761 gcagtgatta aagatttaac aacagaacaa aaagcccgac gacccacgcg gcccacccgc
    56821 gcatcaaaag agcgcagact ggcatcgaaa gcacaaaaat caagcgtgaa ggcgatgcgc
    56881 ggcaaagtgc gcagcggtcg ggaataaaaa gaaggaatgg atggtgaaaa aagcgatagt
    56941 gacagcgatg gctgtaatca gcctctttac tctgatggga tgtaataatc gggccgaagt
    57001 cgatacgctt tctccggcgc aggctgccga actgaaaccg atgccgcaaa gttggcgcgg
    57061 cgtgctgccg tgtgccgatt gcgaagggat cgaaacctct ttgttcctcg aaaaagatgg
    57121 aacctgggtg atgaatgaac gctatcttgg tgcgcgtgaa gagccttcct ctttcgcttc
    57181 ctacggtaca tgggcgcgaa ccgctgacaa gctggtatta accgacagca aaggtgaaaa
    57241 gtcatattat cgggcgaaag gcgatgcgct ggagatgctc gatcgtgaag gtaatccgat
    57301 tgaatcgcag ttcaactata cgctggaacc ggcgcaatcc agtttaccta tgacgccgat
    57361 gaccctgcgg ggcatgtatt tttatatggc tgatgcggcg accttcactg attgcgcgac
    57421 cggaaaacgt ttcatggtag cgaataacgc agagctggag cgtggctacc tgactgcgcg
    57481 cgatcatagt gaaaaaccga tgttactgtc attagaaggg cactttacgc ttgaggctaa
    57541 tccggatacc ggtgcgccaa ccaaagtatt agcgcccgat acggcaggta aattttaccc
    57601 gaaccaggat tgcagtagtt tggggcagta aattgaaagc cggagcattc tccggctttc
    57661 atcactgagt cagcccaaca aagcggcccg ccttgatata aatcccaggc ttcaggtgcc
    57721 caacatactg taactgcgtt tctggcttaa acgcagagac atcgccagta cgcatatgca
    57781 tcagatcggc agggctaatc cagcccgatt gcgccagtgt aagtggatgc ccggctttgg
    57841 caaatgcatc agtgacaaat tccgaacaaa accacgactt tttgtctcct tcgcccacac
    57901 tgcttaactg cgctttcgcc aggccgctga cgcactgttg acgaaaatcc tcggagaacg
    57961 gattcagtga gcacatctgg cgagttacca taaagggaat aaattcgaca atgccgcgat
    58021 agttgtaacc gctatcttta attttgttgg cgaacgcggt gatttctgtg gcttgttgcg
    58081 gggtaagatc cgggactcgt aggacgaaaa gcttatcgct atgcttcatc gcttttttga
    58141 gggaaacaat ctggacgcca gcgcctgtcg cttctgcaac gttattttca cccagataga
    58201 ttgcaacgtg actcacagag gaagtgctga agacgcggat tccaaatgag gttaccccat
    58261 ggcttgagga gaacagcaaa tcgccgggtt tgagatcagg ttccgttatt tcttttattg
    58321 attgttcggt aaaagagctt tgatgctgga atttgactgc ccatgttttc gccccggcat
    58381 ctaccgctgt ggctgatgag tccggctggc tgatatctac ggtacaggcc gaaaggaaaa
    58441 gaaagcaggg gagaagcagg cggcaatgca cctttggttt atccatttta tacagtccat
    58501 gtaaaaaagg gccctgaaat tcaggaccct ttctggcatc agcctttaat ctgtttcacc
    58561 agatattcga cgatgtcacc agtcttaatt aactgtttct cgccgttgcg acgatattta
    58621 tattcgatat cgtcgttgtc gaggttacgg tcgcccagca caatagtgtg cggaataccg
    58681 atcagttcca tatcagcaaa catcacgccc ggacgctctt tgcggtcatc cagcagcact
    58741 tcgatacctt gtgcacgcag ttcgctgtac agtttctcgg ccagctcctg tacgcggaag
    58801 gatttgtgca tgttcatcgg cagaatcgcc acctggaacg gcgcgatagc gtcaggccat
    58861 acgatgccgc gttcgtcata gttctgctca attgccgcag ccacaacgcg cgttaccccg
    58921 ataccgtagc aacccatcgt caggatttga ttacggccat cttcaccctg aacggaggct
    58981 ttcagtgctt cggagtactt ggtacccagc tggaagatgt gaccgacttc gataccacgt
    59041 ttgatcagca gcgtaccctg accatccggg cttggatcgc cagccaccac gttgcggata
    59101 tcagcaattt ctggggtagc gacatcgcga tcccagttga taccgaagta gtgtttacca
    59161 tcgatgttag caccagcagc gaaatcactc atcgccgcaa cggtacggtc aatcaccact
    59221 ggaatcggca tgtttaccgg acccagtgaa cccggaccgg ctttaaccac ggcacgaatt
    59281 tcttcttcgg tcgcgaaagt cagcgggctt gcaacctgcg gcagtttttc tgctttaact
    59341 tcgttcagct cgtgatcacc gcgcaccagc agcgcaacca gcgggaagct actgccttca
    59401 accgctttaa ccagcagagt cttcaccgtt ttctcaatcg gcagattgaa ctgttcaacc
    59461 agttccgcga tggttttcgc gttcggcgta tcaaccagcg tcatttcctg ggtagcagca
    59521 gcgcgcggtt ctttcggcgc gatagcttct gccagctcaa tgttcgctgc atagtcagag
    59581 gtgtcggaga agaccacatc gtcttcaccg ctctgcgcca gcacctggaa ttcgtgagag
    59641 gcgctaccgc cgatagaacc ggtgtcggcc tgtacggcgc ggaaatccag ccccatgcgg
    59701 ctgaagattt tgctgtaggc cgcatacatt gcatcgtagg tttcctgtaa ggattcctga
    59761 gaagtatgga aagagtaagc atctttcatc aggaattcgc gggaacgcat gacgccgaaa
    59821 cgcggacgca cttcgtcgcg gaacttggtc tggatctgat agaagttcag cggcagctgt
    59881 ttgtaagagc tcagctcgtt acgaatcagg tctgtaataa cttcttcatg agttgggccg
    59941 agtacgaacg gacgctcgcc acggtcaaca aaacgcagca gttccggacc gtactgttcc
    60001 caacgaccac tctcttgcca caaatcggct ggctgaacca ccggcatcga cacctcgatc
    60061 gcaccggcgt tgttcatctc ttcacgcacg atgttttcga cttttttcag aacgcgcacg
    60121 ccggtcggca gccaggtata taacccggag gccagcttgc ggatcatccc ggcgcgcagc
    60181 atcagctgat ggctgatcac ctcggcgtcg gcaggtgtct ccttgagagt ggagagcagg
    60241 tattggctag tacgcatgtt gttacggttc cagttggaag gtagaacagg ctcaaggcga
    60301 gcctgggaca aaaaaagtga tttagtttac cagtgcaaaa gaaatgtcaa aagagaaggg
    60361 cgcgaattta acgcggttcc agcgcaaaga cttcaaaacc tgcgtcggtg acgcgccagc
    60421 gaacgttaaa atcatgcagc cagacggcat aggttttgcc cgtttcctca cctttacgat
    60481 aggccgggcg cgggtcctgc gccagtactt cgcggataaa cagcgttaac tgcggataac
    60541 gtttctccag cgtcaaaagc tgcttttcga cctctgcggt aaagctcacc gccatctctg
    60601 ccgctggcgc actttgcgca tagctggcac tggcatttgg aagcgattcg gcaaagggga
    60661 gatacggttt gatatccacc accggcgtac catctaccag atccagactg ccgagcttca
    60721 gaatcacgct gtctttatgg caaacaacct ctttcagctc taccagcgac ataccaatcg
    60781 ggttagggcg gaaagtggag cgcgtagcga aaacacccat tctggcatta ccgccgaggc
    60841 gcggtggacg cacagtcgga cgccagccgc cttccatcgt ttgatgaaag acgaaaagga
    60901 tccataaatg gctgaacgct tccaggccgc gaacggcatc ggcctggttg tagggagcaa
    60961 tgagatgcag ttcgccgttg gcgcttttta ccagacctgg ctggcgcgga acggcgaatt
    61021 tttctttata gggcgagcga ataacgccta tttgctcgaa ctggaaactg ctcatttcgc
    61081 cgtaatgtta agcgcagaac cgatacatac agcctgacga tagcagcctg gcgtaccgct
    61141 ggtgacttcg cagctatgca gtaataccgc attggccttc attttcgagg cgttgatttg
    61201 catccgctta cgtgcggttg gaatgctcgg cggagagtcc tgattggagg cctggcaaga
    61261 atcgccactg acttcaccga gatcgcggaa cggtttgccg actaattctt ctgcattggt
    61321 ataaattcgg accggcgtgg cgcgcggcgc tttcggtttt gcaggctccg ctttcggctg
    61381 gggtgcagtg ctttgaacgg gttcgacagg ggatctgctt aacatggaac agccgcttag
    61441 catgagtgct actaaacaga tcggtaaagc acgcatagta tttcctcaat gtatgatcaa
    61501 aacgtcaata ttgaatcagg agcttgtaaa aatgacaaga cgggcaagcg cccgtcctga
    61561 atgatattac aaattgtgga aacagcctaa aaattaccag cctttaacag cgccgccgtt
    61621 aaacactttg tttgctgctt cgtaaacttc gtcagactga taagcctgga cgaatttctt
    61681 cacgttctcg gcgtctttgt tatcttcacg cgtcacgatc aggtttacgt acggggagtc
    61741 tttatcttcg acaaagatac cgtctttcgc cggagtcagg ccaatctggc tggcataggt
    61801 ggtattgata actgccagag cgatttgcgc gtcgtccaga gagcgaggca gctgcggtgc
    61861 ttccagctca acaattttca gattttttgg gttctcaaca acatcaagaa cggtcggcag
    61921 caggccaacg ccatctttca gtttgatcaa gcccactttt tgcagcagca gcagtgaacg
    61981 accaaggtta gttgggtcgt ttggcacggc aacctgcgaa ccatcctgca attcatccag
    62041 tgatttgatt ttcttggagt aaccagcaat cggataaaca aaagtgttgc ctactgcgac
    62101 cagtttgtag ccacgatctt tcagttgctg atcaaggtac ggtttatgct ggaaggcgtt
    62161 ggcgtcgata tcgcctttgc tcaatgcctc gtttggcaga acatagtcgt tgaaggttac
    62221 cagctcaacg tccagaccat atttgtcttt cgcaactttc tgcgcgactt ctgcaacctg
    62281 ctgttcggca ccaacaatca cgccgacttt aatgtggttt ggatcttttt catcctgacc
    62341 gcagcctacc aatgccagtg atccaatcag ggctcccact gccgcaaagg ttttgaattt
    62401 gaacgccata ccttattcct tcttcaatta tttatgttgt gttgaacgtt acttgcgagt
    62461 gacagcccgg acgatgcggt cgcctgcgaa ctgaattaaa taaaccagaa tgaccagcaa
    62521 taccagtacc gtattcatca ccgtcgcgtt gtagccgatg tagccatact gatagccaat
    62581 ctgacctaaa ccaccggcac cgactgcacc acccatcgcg gaataaccga ccagggtaat
    62641 aagggtgata gttgccgcat tcaccagacc cggcaacgct tccggtaaca gcactttacg
    62701 gacgatctgc attggcgtgg cacccattgc gcgggaagct tcaattaacc cggttgggat
    62761 ctccagcaga gcgttctcga ccatacgggc aataaacggt gctgcaccaa cggttaacgg
    62821 aacaatcgct gcctgcaaac caatcgatgt accgacaata acgcgggtaa acggaatcat
    62881 ccataccagc aagataatga acgggataga acggaaaatg ttcacaatcg cagaaacggt
    62941 acgatacagc ttcgcgttag caataatttg ccccgggcgc gtaacataaa gcaggacgcc
    63001 gaccggcaga ccaatcacaa acccaaaaaa tccggatacg aaggtcattg ccagcgtttc
    63061 ccatacgcca cgaaccagca gccacatcat cggctcagac ataacccagt acctctactt
    63121 ttacatggtg ttcctgcagc caggcaatgg cggcttgcgt atcttgttgt gtgccgtgca
    63181 tttcagtcag catgatgccg aacttaacgc caccggcgta atccatctgc gcgctaataa
    63241 tgttgttgtt gacgttgaaa cgacgcgcgg tttcagaaag cagtggggca tcgaccgatt
    63301 gaccggtaaa ctccagacgc agcatcggca cgcagtcagt gaatggctcc gcttgcagac
    63361 gctcctgata atcttccggg atatccagat gcagggtcga ctgaataaac ttctgcgcca
    63421 gcggcgtttt cggatgcgag aacacttcac ttaccgtgtc ctgctcgatc agttcgccat
    63481 tactgataac cgccacgcaa tcacaaatgc gcttcacaac gtccatttcg tgggtgatca
    63541 acagaatcgt caaacccaga cggcggttaa tgtctttcaa cagttcgaga atagaacgtg
    63601 tcgttgccgg gtccagcgcg ctggtggctt catcacacag caatactttg ggattgctgg
    63661 ctaatgcacg ggcaatcgcc acacgttgtt tctgcccacc ggaaagattc gacgggtagc
    63721 tatcatgctt atcgccaaga ccaaccaatg acagcaattc cgtcacgcga cgtttgatct
    63781 cgtctttcgg tgtattgtcc agctccagcg gcagagccac gttgccaaaa acagtacgcg
    63841 aagagagcag gttaaaatgc tggaaaatca taccaatctg gcggcgagct ttggtcaact
    63901 cggattctga cagcgtggtc agttcctgtc catcgaccag cacgctaccc tcggttgggc
    63961 gctccagcag gtttacacaa cgtataagcg tactcttacc cgcgcctgag gcaccgataa
    64021 cgccataaat ttgtccagct ggcacatgca ggctgacgtt gttcaatgcc tggatggtgc
    64081 gggtgccctg gtggaacact ttggtgatat tcgaaagttt tatcattgat tatttattat
    64141 cgtcattaag ttagtcgtgg catttcgaat gcctggaacg ggcaacgccg tcaatgaaat
    64201 ggatgttaag gcatccagac gtctaaatca atcaggttta tgcgaagagc actttcttgc
    64261 tggtcgaaac atgcgatact agcgtcacat gccttattaa ggagctataa aggtggcgaa
    64321 gagcgtaccc gcaatttttc ttgaccgtga tggcaccatt aatgtcgatc acggctatgt
    64381 ccatgagatc gacaactttg aatttatcga cggtgttatt gacgccatgc gcgagctaaa
    64441 aaaaatgggc tttgcgctgg tggtagtaac caaccagtct ggcattgctc gcggtaaatt
    64501 tactgaagca cagtttgaaa cgctgaccga gtggatggac tggtcgctgg cggaccgcga
    64561 tgtcgatctg gatggtatct attattgccc gcatcatccg cagggtagtg ttgaagagtt
    64621 tcgccaggtc tgcgactgcc gcaaaccaca tccggggatg cttttatcgg cacgcgatta
    64681 tttgcatatt gatatggccg cttcttatat ggtgggcgat aaattagaag atatgcaggc
    64741 agcggctgcg gcgaacgtgg gaacaaaagt gctggtgcgt acgggtaaac ctgttacgcc
    64801 tgaagcagaa aacgcggcgg attgggtgtt aaatagcctg gcagacctgc cgcaagcgat
    64861 aaaaaagcag caaaaaccgg cgtaatgatt aaaagatgag cggttgaaat aaaaatgcat
    64921 ttttccgctt gtcttcctga gccgactccc tataatgcgc ctccatcgac acggcggatg
    64981 tgaatcactt cacacaaaca gccggttcgg ttgaagagaa aaatcctgaa attcagggtt
    65041 gactctgaaa gaggaaagcg taatatacgc cacctcgcga cagcaagctg aaagccgcgt
    65101 cgcaactgct ctttaacaat ttatcagaca atctgtgtgg gcactcgaag atacggattc
    65161 ttaacgtcgc aagacgaaaa atgaatacca agtctcaaga gtgaacacgt aattcattac
    65221 gaagtttaat tctttgagca tcaaactttt aaattgaaga gtttgatcat ggctcagatt
    65281 gaacgctggc ggcaggccta acacatgcaa gtcgaacggt aacaggaaac agcttgctgt
    65341 ttcgctgacg agtggcggac gggtgagtaa tgtctgggaa actgcctgat ggagggggat
    65401 aactactgga aacggtagct aataccgcat aa
//
LOCUS       DACBVI010000009        63177 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.009, whole genome shotgun sequence.
ACCESSION   DACBVI010000009 DACBVI010000000
VERSION     DACBVI010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.009"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..96
                     /locus_tag="IU325_02930"
     CDS             <1..96
                     /locus_tag="IU325_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5807993.1"
                     /translation="FTKIILIFSVFVFNYNESQITDAAVNLFIQL"
     gene            110..982
                     /locus_tag="IU325_02935"
     CDS             110..982
                     /locus_tag="IU325_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HAP5807994.1"
                     /translation="MRYFILIFTFVCSFVAAQPTIVPSLQQQVTDLTSSLNSQEKKEL
                     TYKLESIFNNTQVQIAVLIVPTTKDETIEKYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQY
                     PANPSEIESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSSVCISAIYLLDL
                     FLFSDKTFSSAVFFFFFTFTTIMVFTCLCVLQKKASGRSYHSDNSGSGGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(1042..2340)
                     /locus_tag="IU325_02940"
     CDS             complement(1042..2340)
                     /locus_tag="IU325_02940"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP5807995.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2428..4065)
                     /gene="pyrG"
                     /locus_tag="IU325_02945"
     CDS             complement(2428..4065)
                     /gene="pyrG"
                     /locus_tag="IU325_02945"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HAP5807996.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4293..5084)
                     /gene="mazG"
                     /locus_tag="IU325_02950"
     CDS             complement(4293..5084)
                     /gene="mazG"
                     /locus_tag="IU325_02950"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HAP5807997.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVIDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5156..5491)
                     /gene="mazF"
                     /locus_tag="IU325_02955"
     CDS             complement(5156..5491)
                     /gene="mazF"
                     /locus_tag="IU325_02955"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HAP5807998.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(5491..5739)
                     /gene="mazE"
                     /locus_tag="IU325_02960"
     CDS             complement(5491..5739)
                     /gene="mazE"
                     /locus_tag="IU325_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HAP5807999.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(5817..8051)
                     /gene="relA"
                     /locus_tag="IU325_02965"
     CDS             complement(5817..8051)
                     /gene="relA"
                     /locus_tag="IU325_02965"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HAP5808000.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKISGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(8099..9400)
                     /gene="rlmD"
                     /locus_tag="IU325_02970"
     CDS             complement(8099..9400)
                     /gene="rlmD"
                     /locus_tag="IU325_02970"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP5808001.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKALFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASEDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVAGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     CALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNDLH
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9457..12213
                     /gene="barA"
                     /locus_tag="IU325_02975"
     CDS             9457..12213
                     /gene="barA"
                     /locus_tag="IU325_02975"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HAP5808002.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12454..13794)
                     /gene="gudD"
                     /locus_tag="IU325_02980"
     CDS             complement(12454..13794)
                     /gene="gudD"
                     /locus_tag="IU325_02980"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAP5808003.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDKCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAKRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PQKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13815..15155)
                     /locus_tag="IU325_02985"
     CDS             complement(13815..15155)
                     /locus_tag="IU325_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase-related protein"
                     /protein_id="HAP5808004.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQREAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTQNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15157..16509)
                     /locus_tag="IU325_02990"
     CDS             complement(15157..16509)
                     /locus_tag="IU325_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5808005.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFFWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16944..17393)
                     /locus_tag="IU325_02995"
     CDS             complement(16944..17393)
                     /locus_tag="IU325_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP5808006.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDTLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTLLS"
     gene            complement(17411..18193)
                     /gene="truC"
                     /locus_tag="IU325_03000"
     CDS             complement(17411..18193)
                     /gene="truC"
                     /locus_tag="IU325_03000"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HAP5808007.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18193..18522)
                     /locus_tag="IU325_03005"
     CDS             complement(18193..18522)
                     /locus_tag="IU325_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808008.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWQNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLENNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19144..19689)
                     /locus_tag="IU325_03010"
     CDS             complement(19144..19689)
                     /locus_tag="IU325_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HAP5808009.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRAHLASNLAEFLNQLKPLL"
     gene            19757..20605
                     /gene="queF"
                     /locus_tag="IU325_03015"
     CDS             19757..20605
                     /gene="queF"
                     /locus_tag="IU325_03015"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HAP5808010.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20717..22081
                     /gene="ppnN"
                     /locus_tag="IU325_03020"
     CDS             20717..22081
                     /gene="ppnN"
                     /locus_tag="IU325_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HAP5808011.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            22637..23926
                     /locus_tag="IU325_03025"
     CDS             22637..23926
                     /locus_tag="IU325_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAP5808012.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFN"
     gene            23984..25351
                     /locus_tag="IU325_03030"
     CDS             23984..25351
                     /locus_tag="IU325_03030"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAP5808013.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQDTGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            25372..26217
                     /gene="xni"
                     /locus_tag="IU325_03035"
     CDS             25372..26217
                     /gene="xni"
                     /locus_tag="IU325_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HAP5808014.1"
                     /translation="MRGLFPFSQPAIACSGIECYPYRLIFKGVIVAVHLLIVDALNLI
                     RRIHAVQGSPCVETCQHALDQLIMHSQPTHAVAVFDDENRNSGWRHQRLPDYKAGRPP
                     MPEELHDEMPALRTAFEQRGVPCWSASGNEADDLAATLAVKVTQAGHQATIVSTDKGY
                     CQLLSPTLRIRDYFQKRWLDAPFIDKEFGVQPQQLPDYWGLAGISSSKVPGVAGIGPK
                     SATQLLVEFQSLEGIYENLDAVAEKWRKKLETHKEMAFLCRDIARLQTDLHIDGNLQQ
                     LRLVR"
     gene            complement(26272..27420)
                     /gene="fucO"
                     /locus_tag="IU325_03040"
     CDS             complement(26272..27420)
                     /gene="fucO"
                     /locus_tag="IU325_03040"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP5808015.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(27448..28095)
                     /gene="fucA"
                     /locus_tag="IU325_03045"
     CDS             complement(27448..28095)
                     /gene="fucA"
                     /locus_tag="IU325_03045"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HAP5808016.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            28642..29958
                     /locus_tag="IU325_03050"
     CDS             28642..29958
                     /locus_tag="IU325_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose-proton symporter"
                     /protein_id="HAP5808017.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            29991..31766
                     /gene="fucI"
                     /locus_tag="IU325_03055"
     CDS             29991..31766
                     /gene="fucI"
                     /locus_tag="IU325_03055"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HAP5808018.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYSHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            31875..33293
                     /gene="fucK"
                     /locus_tag="IU325_03060"
     CDS             31875..33293
                     /gene="fucK"
                     /locus_tag="IU325_03060"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HAP5808019.1"
                     /translation="MKQEVILVLDCGATNVRTIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSKLTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQQLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            33295..33717
                     /gene="fucU"
                     /locus_tag="IU325_03065"
     CDS             33295..33717
                     /gene="fucU"
                     /locus_tag="IU325_03065"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HAP5808020.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            33775..34506
                     /gene="fucR"
                     /locus_tag="IU325_03070"
     CDS             33775..34506
                     /gene="fucR"
                     /locus_tag="IU325_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HAP5808021.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(34550..35650)
                     /gene="rlmM"
                     /locus_tag="IU325_03075"
     CDS             complement(34550..35650)
                     /gene="rlmM"
                     /locus_tag="IU325_03075"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HAP5808022.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(35643..36038)
                     /locus_tag="IU325_03080"
     CDS             complement(35643..36038)
                     /locus_tag="IU325_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HAP5808023.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(36057..36974)
                     /gene="gcvA"
                     /locus_tag="IU325_03085"
     CDS             complement(36057..36974)
                     /gene="gcvA"
                     /locus_tag="IU325_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HAP5808024.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(37325..37552)
                     /locus_tag="IU325_03090"
     CDS             complement(37325..37552)
                     /locus_tag="IU325_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HAP5808025.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            37744..38949
                     /gene="csdA"
                     /locus_tag="IU325_03095"
     CDS             37744..38949
                     /gene="csdA"
                     /locus_tag="IU325_03095"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HAP5808026.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            38949..39392
                     /gene="csdE"
                     /locus_tag="IU325_03100"
     CDS             38949..39392
                     /gene="csdE"
                     /locus_tag="IU325_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HAP5808027.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTMAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAVAKQV"
     gene            complement(39443..40249)
                     /gene="tcdA"
                     /locus_tag="IU325_03105"
     CDS             complement(39443..40249)
                     /gene="tcdA"
                     /locus_tag="IU325_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HAP5808028.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(40325..41422)
                     /gene="mltA"
                     /locus_tag="IU325_03110"
     CDS             complement(40325..41422)
                     /gene="mltA"
                     /locus_tag="IU325_03110"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HAP5808029.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            41630..41706
                     /locus_tag="IU325_03115"
     tRNA            41630..41706
                     /locus_tag="IU325_03115"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41664..41666,aa:Met,seq:cat)
     gene            complement(41781..43034)
                     /locus_tag="IU325_03120"
     CDS             complement(41781..43034)
                     /locus_tag="IU325_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HAP5808030.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            43266..44597
                     /locus_tag="IU325_03125"
     CDS             43266..44597
                     /locus_tag="IU325_03125"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HAP5808031.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(44659..46485)
                     /gene="recD"
                     /locus_tag="IU325_03130"
     CDS             complement(44659..46485)
                     /gene="recD"
                     /locus_tag="IU325_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HAP5808032.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFSD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(46485..50027)
                     /gene="recB"
                     /locus_tag="IU325_03135"
     CDS             complement(46485..50027)
                     /gene="recB"
                     /locus_tag="IU325_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HAP5808033.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDAVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDAITAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(50020..52908)
                     /locus_tag="IU325_03140"
     CDS             complement(50020..52908)
                     /locus_tag="IU325_03140"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HAP5808034.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRTMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(53084..56452)
                     /gene="recC"
                     /locus_tag="IU325_03145"
     CDS             complement(53084..56452)
                     /gene="recC"
                     /locus_tag="IU325_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HAP5808035.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLEGSQELDAFVDVTPDNL
                     LHNIQSDILELENCAVAGVNIEEFSRSDNKRLLDPLDNSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESTQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(56465..56788)
                     /locus_tag="IU325_03150"
     CDS             complement(56465..56788)
                     /locus_tag="IU325_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP5808036.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPAHWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(56773..57180)
                     /locus_tag="IU325_03155"
     CDS             complement(56773..57180)
                     /locus_tag="IU325_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HAP5808037.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALEWGKMHSWQTQPAVQCLLYAATGARVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(57177..57740)
                     /locus_tag="IU325_03160"
     CDS             complement(57177..57740)
                     /locus_tag="IU325_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAP5808038.1"
                     /translation="MPVKELGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(57731..58201)
                     /locus_tag="IU325_03165"
     CDS             complement(57731..58201)
                     /locus_tag="IU325_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAP5808039.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(58386..59180)
                     /gene="thyA"
                     /locus_tag="IU325_03170"
     CDS             complement(58386..59180)
                     /gene="thyA"
                     /locus_tag="IU325_03170"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP5808040.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(59187..60062)
                     /locus_tag="IU325_03175"
     CDS             complement(59187..60062)
                     /locus_tag="IU325_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP5808041.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(60213..62459)
                     /gene="ptsP"
                     /locus_tag="IU325_03180"
     CDS             complement(60213..62459)
                     /gene="ptsP"
                     /locus_tag="IU325_03180"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP5808042.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(62472..63002)
                     /gene="rppH"
                     /locus_tag="IU325_03185"
     CDS             complement(62472..63002)
                     /gene="rppH"
                     /locus_tag="IU325_03185"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HAP5808043.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
BASE COUNT        15260 a        17058 c        15472 g        15387 t
ORIGIN      
        1 ttcaccaaaa ttatacttat tttttctgtg ttcgttttta attataacga atctcaaatt
       61 accgatgccg ccgtcaattt atttatccag ctttaatggg acatttataa tgcgctattt
      121 catactaata ttcacattcg tgtgttcctt tgttgcagcc caaccaacta ttgtcccatc
      181 attacagcaa caagttacgg atcttacgag tagcttaaac tcacaagaaa agaaagaact
      241 gacgtacaag ttagaatcta ttttcaataa tacccaagtg caaattgctg tattaattgt
      301 tcccacaacc aaagacgaga caattgaaaa atatgctaca cgggtttttg acaattggcg
      361 tttaggagat gccaaacgta atgatgggat actgatcatt gttgcctggt cggatcgtac
      421 tgtccgcatc caggtaggtt atgggctgga agaaaaggta accgatgctc tggctggaga
      481 tatcatccgt agcaacatga tacccgcatt taaacaacaa aaattagctc agggattaga
      541 gctagctata aacgctttga ataaccaact cacttcacaa caccaatatc cggctaatcc
      601 ttcagaaatt gaatcagcgt cttccagtga tcattattac tttgctattt tttgggtatt
      661 tgcagtgatg ttcttccctt tctggttttt tcatcaaggc agtaattttt gtcgcgcatg
      721 taaaagcagt gtctgtattt cagcaatcta tcttttagat ttattcctgt tctccgataa
      781 aactttttcc agtgctgtat ttttcttctt ttttactttc accaccatta tggtctttac
      841 ctgtttatgt gtacttcaga aaaaagcatc tggtagaagt tatcattcag acaacagcgg
      901 ttccggtggc ggctcaagtg gcggcggatt tagcggcggg ggcggttctt ctggcggtgg
      961 cggcgcgtcc ggtcgctggt aactcaacca taaaaaatgc cagcccgaag gctggcattt
     1021 ttaaatcaga taaagtcaga cttatgcctg gcctttgatc tctttacgac cgttgtacgg
     1081 tgctttttcg cccagagctt cttcgatacg aatcagctgg ttgtatttag caacgcggtc
     1141 agaacggctc atagaaccag ttttgatctg gcctgcagca gtaccaacag ccaggtcagc
     1201 gatggtagca tcttcagttt cgccagaacg gtgagagatt actgcagtgt agccagcgtc
     1261 tttcgccatc ttgattgcag ccagagtttc ggtcagagaa ccgatctggt tgaatttgat
     1321 caggatggag ttagcgatac ctttttcgat accttctttc aggatcttgg tgttggttac
     1381 gaacaggtcg tcaccaacca gctggatttt gtcgcccaga actttggtct ggtatgcgaa
     1441 accgtcccag tcagattcgt ccagaccgtc ttcgatggat acgatcggat actgtttggt
     1501 cagttcttcc aggaagtgag tgaattcttc agaggtgaat gctttgttgc cttcgccagc
     1561 cagaacgtat ttaccgtctt tgtagaattc agaagctgcg cagtccatcg ccagagtgat
     1621 gtctttgccc agttcatagc cagcagcttt aacagcttca gcgataacag ccagagcttc
     1681 agcgttggaa cccaggttcg gcgcatagcc accttcgtca ccaacagcag tgttcatgcc
     1741 tttcgctttc agaacttttg ccaggtgatg gaaaacttca gaacccatgc ggatggcttc
     1801 tttcacagtt ttcgcgccaa ccggctgaat catgaattcc tggatatcaa cgttgttgtc
     1861 agcgtgctca ccaccgttga tgatgttcat catcggaacc ggcatggagt atttgcccgg
     1921 agtgccgttc agttcagcga tgtgctcgta cagcggcata cctttagcag ctgcagcagc
     1981 tttggcgtta gccagagata cagccaggat tgcgttcgcg ccgaatttgg atttgttttc
     2041 ggtgccgtcc aggtcgatca tgatcttgtc aatgccagcc tgatctttgg catctttgcc
     2101 aatcagtgcc tgagcgatcg ggccgtttac cgcagcaaca gctttggtta cgcctttacc
     2161 caggaaacgg gatttgtcgc catcgcgcag ttccagagct tcacgggaac cagtagaagc
     2221 acctgacgga gcagctgcca taccgacgaa accaccctcc agatgtactt cggcttcaac
     2281 agtcgggtta ccacgggagt cgatgatttc acgaccgatg atttttacga ttttggacat
     2341 taggttttcc tcaagtcact agttaaactg aaactccaga caaacaacgc gtacccaggg
     2401 tacgcgttgc cgttctaact tttttactta cttcgcctga cgtttctgga actcgctggc
     2461 ggctttcaca aagcctgcaa acagcgggtg accatcacgt ggagtagaag taaactccgg
     2521 atggaactgg caagccacga accacgggtg attcggaact tcgatgatct cgaccaactg
     2581 atcatccccg gaacggcccg caacgcgcag acctgcatct tcaatctgtt tcaacagcat
     2641 gttgttgact tcgtaacggt gacgatgacg ctcaacaatt gtcggcgcat tgtacagctg
     2701 gcgaaccagg ctatcgtcaa ccaactggca ctgctgtgcg ccgagacgca tggtaccgcc
     2761 gagatcgctc ttctcgctac gaacttcaac gttgccgttt tcatcgcgcc actcggtaat
     2821 cagcgccaca accgggtact tacagtctgg cacaaattcc gtagagttgg cgttctccat
     2881 gttggcaaca tggcgagcgt aatcaattaa cgccacctgc atacccaggc aaatgcccag
     2941 ataaggaata ttgttctcac gcgcaaaacg cgcggtcgta atcatgcctt ctacgccacg
     3001 atagccgaaa ccgccaggta cgaggattgc gtccagacct ttaagaattt caacgccgcg
     3061 cgtttcaaca tcttgtgaat cgatcagttt gatgttgacg ctgacacgat tcttcagccc
     3121 accgtgtttc agtgcttcga tcacggattt gtaagcgtcc ggcagttcaa tgtacttgcc
     3181 gaccatacca atggtgactt cacttaccgg attcgcttct tcgaagataa cctgttccca
     3241 ttcggacaga ttcgcttccg ggcagtttaa gctgaatcgt ttacaaatat aatcgtccag
     3301 cccctgcgat ttcaacaggc ccggaatttt atagatggaa tcgacgtctt tcagagaaat
     3361 aaccgctttt tccggaacat tacagaacaa tgcaatcttc gcacgttcgt tcgccggaac
     3421 ggcgcgatct gaacgacaaa tcaggatgtc aggctggata ccgatggaga gcagctcttt
     3481 tacagagtgc tgagtcggtt tggttttgac ttcaccagac gctgccatgt acggcaccag
     3541 cgtcaggtgc ataaacaggg tgtgctcacg gccaatttca acagccatct ggcgaatcgc
     3601 ttcgaggaac ggcagggatt cgatatcacc tactgtaccg ccgatttcga ccagtactac
     3661 gtcatgacct tcgccgcctt ccagcacgcg ctctttgata gcgttagtga tgtgcggaat
     3721 aacctgtacg gttgcgccga ggtagtcgcc acggcgttct ttacgcagaa cgtcagagta
     3781 gatacgaccc gtggtgaagt tgttgcggcg gctcattttg gtacgaatga aacgctcgta
     3841 gtgccccagg tccaggtcgg tttcagcgcc gtcttcagta acgaacactt ccccgtgttg
     3901 gattgggctc atagtacctg gatcgacgtt gatgtacgga tccagtttca tgatggtcac
     3961 attgaggcca cgggcttcaa gaatggctgc gagggaggct gcggcaatgc ctttacccag
     4021 agaggatacg accccgccgg tcacaaaaat atagttcgtt gtcatgctga acctgagaag
     4081 ttaggttgaa aagacgatgg aataaccagg acgggaaagc agtatacccg aacatgacca
     4141 gtgccacaaa ctttcattat ccctcctctt cgccagcgca ctattgaaat caggagtgag
     4201 aaaatagccc ctttggggta aatgtttttg acgcaaatca agcgcttgtc atttaaaaaa
     4261 tgacacaaat cgcgcttgac cacgtaattc ccttagagat caatttcctg ccgttttacc
     4321 tgttgccaga cttcttccat tgtttcgagg tcaacacctg tcatttccag tccacgcgcg
     4381 gcaacaatac gctccacttc gcgaaaacga cgctcgaatt tttcgttcgc tttttgcaat
     4441 gcgatttccg cttttgtccc taaatgacga gccagattga ccgtggcaaa cagcaggtcg
     4501 cccatttcct cctccagttt agcctggtcg ataacagcct gccgtgcctc gtacatcacc
     4561 tcgtcgatct cttcgtagac tttatcgact accggaccaa gcgtcgtcca gtcaaaacca
     4621 acgttggcgc aacgtttctg gattttttgc gcacgcatta aagccggtaa actacgagga
     4681 atatcgtcca gcgccgaatg ctgcgctttc tgcgcgcgct cttcggtttt gatttgctcc
     4741 caacgggcaa gcacttcact actgttttcg gcagaactgt cagcaaaaac atgcggatgg
     4801 cgacgttcta atttatcgct gatcgcggcg catatatcat taaagtcaaa gcgcccttct
     4861 tcctgagcca tttgtgcgta aaacaccacc tggaacagca gatcgcccag ttcaccgcga
     4921 agatcgtcaa aatcttcacg ggcgatggcg tccagcacct cgtaggtttc ttcaagggtg
     4981 taaggcgcaa tggtggcaaa tgtctgctct ttatcccacg ggcagccgtt ttccggatcg
     5041 cgcaggcgct gcatgatggt gagcaaacgg tcgatttgat tcattagaat gtcctgaaaa
     5101 ttgcgggtcc gtcaggtgga aacctgtgac cagaatagaa gtgagttagt aacacctacc
     5161 caatcagcac gttaattttg gctttaatga gctgtaattc ctctggggca accgttcctt
     5221 tcttcgttgc tcctcttgcc cgccaggcga tactttttac ctgatcagct aacgctacgc
     5281 catcacgttc ctgaccggat aaaacaactt cgaacggata tccttttgat tgcgttgtac
     5341 aaggaacaca cagacacata cctgttttgt tgttgtacat gaacggactc aggacaacag
     5401 ccggacgatg tccggcttgc tcgctacctt ttgtcgggtc aaaatcaacc caaatcagat
     5461 cgcccatatc gggtacgtat cggcttacca ttaccagact tccttatctt tcggctctcc
     5521 ccagtcgata ttctcgtgga ggttttccgg cgtgatgtcg ttgaccagtt cagcaagcgt
     5581 aaatacgggc tctttacgca ctggctcaat aattaatttg ccatccacca ggtcaatctt
     5641 cacttcatca tcaatattca gattgagcgc ctgcattaac gtagccggga tccgcaccgc
     5701 cggtgaattt ccccaacgct ttacgctact gtggatcata accctttcct caaaccgcta
     5761 tcatatgtag atacagtata tatcaatcta cattgtagat acgagctaat ttcggcctaa
     5821 ctcccgtgca accgacgcgc gtcgataaca tccggcacct ggttgagttt acccagcacg
     5881 cgacccagca cctgcaggtt gtaaatctca atggtcatgt cgatggtcgc cagttgctgt
     5941 ttggtgtcgc tacggctggc aacgccaagc acgttcacct tctcgttggc gagaatggtc
     6001 gtgatatcac gtaacaaccc actacgatca ttagccacca cgcggaccac tagcgaatat
     6061 ccggcggagt agctctcgcc ccataccgcg tcaacaatgc gttctggcgc atgggagcgc
     6121 agttccgcca gttgctcaca gtctgcacga tgcactgaaa taccgcgtcc ctgggtaatg
     6181 aagccgacaa tctcatctcc aggaatcggc tggcagcagc gcgcgatgtg gtgcatcagg
     6241 ttaccgacac cttcaaccac cacacgaccg ttatctttac tgcggttttg cggcgtatag
     6301 cttttttgct gaagttgctt cagcgcggcg gcatcctgct cttcggcact cggcttatta
     6361 aattgcgatt gcaggaagtt caccatctga ttgagacgga tatccccgcc accaattgcc
     6421 gccagcaact cgtcgacatc attgaagttg taacgcggca gcagatgttt ttctgcttct
     6481 ttcaggctga tccccagatg ttccagctcg tcgtcaagga tttgccgccc agccagaatg
     6541 tttttgtcac ggtcctgttt acggaaccag gcgtgaattt tcgaacgccc acggctggtt
     6601 gtgacgtaac cgaggtttgg gtttaaccag tcacggctgg ggttcggctg tttctgggtg
     6661 ataatttcaa tctggtcgcc catctgcagc tggtaggtga acggcacaat gcgcccgcta
     6721 atttttgccc cgatgcagcg gtgcccgaca tcactgtgga tgtggtaagc gaagtccagc
     6781 ggcgttgatc ccgcaggcaa atcaacgacg tcacctttcg gcgtaaagac gtacacccgg
     6841 tcgtcaaaga cctggctgcg tacttcgtcg agcatttcgc cggaatcagc catctcttcc
     6901 tgccacgcaa tcagtttacg cagccaggca atccggtctt catgtcccga acgtgcgccg
     6961 ccagctgccg cgccctcttt atatttccag tgcgcagcaa cacccaactc tgcatcttca
     7021 tgcatctgtt tagtgcggat ttggatctca acggttttgc cacctggtcc cagaaccacg
     7081 gtatgaatag actgataacc gtttggtttc gggttagcga cgtaatcgtc aaactcatcc
     7141 ggcaggtggc gatagtgagt gtgcactatc cccagtgcgg catagcaatc ctgtaaacgc
     7201 tcggcgacaa tacgtaccgc acgcacatca aacagctcat cgaaggcgag gttctttttc
     7261 tgcattttgc gccagatgct gtagatgtgt ttcggtcgac catacacttc agctttaacg
     7321 ccttcagctt tcatctcagc gcgcagatgg ccgacaaact cttcgatata gtgttcgcgg
     7381 tcgagacggc gttcatgcag cagttttgcg atgcgtttgt attcggtcgg gtggagataa
     7441 cggaagcagt aatcttccag ctcccatttc agttgtccaa tcccaagacg gttcgccaac
     7501 ggcgcgtaga tattggtgca ctcttttgcc gccagtacac gttcatcttc cggcgcatct
     7561 tttacttcgc gcagatgagc aatacgctct gccagtttga tgaccacgca gcgaaaatca
     7621 tcgaccatcg ccaataacat ccggcgaacg ttatcgacct gttcggagga aacagaatcg
     7681 gtatgcgtcg ctttcagctg gcggatcgcc gccatatcac gcacgccgtg aataaggtta
     7741 acgaccgact taccgacgct ctcacgcagc acatcttcgc tgactacgtt ggcatcggcc
     7801 agagggaaca acagcgccgc ccgcagcgtg tcaatgtcca tacttaatgt cgagaggatc
     7861 tccaccatct caacaccacg ccacaataac agactggcat ccggatgccc ctgcgtctgt
     7921 tgcagacaat acgcccaggt ttcggctaag cactcacacg acttctggct ggtaataccc
     7981 agacttgcga tccatttttc cggatcaaat tcaccagcct tattgatatg tgcacttctt
     8041 accgcaacca tcgtcctctc ctttagggac cagacctgcc gaaatcggca aatcgtaact
     8101 atttaacgcg cgagaaaagt accatcgatt ccagatgtcc cgtgtgtggg aacatatcca
     8161 gcatcgccag tcgcgcaatg gtatatcctg cttttaataa cgcttcgcta tcccgagcca
     8221 gcgttgcagg gttacaggat acataaacta tacgaatagg ttccagtttt ataatttgct
     8281 gcataacacc tgcggcacct gctcgcgcgg ggtccagcaa cactttatcg aagccgtttt
     8341 tcgcccacgg ctgctttgtg acatcttctt caagattttc gtgataaaac gtcacattgt
     8401 gtaagtcatt aagacgcgca ttctgctggc ctttttccac cagcgccgga acaccttcta
     8461 cacccaccac actggcagct tgtgtcgcca atggcagtgt aaagttgccc ataccgcaga
     8521 acagatccag tacgcgatct tcaggttgca catccagcca ttccaacgca cacgctacca
     8581 ttttttggtt cacacccgca ttgacctgaa taaaatcgcg cgggctaaaa gttaagcgca
     8641 acccgtttga gtcataccag ggcatctcac cagcgacggt ttcgagtatc tcactatcgg
     8701 gggcgagata cagatccagg ccttcagaat gcgaaaagcg ttccagtttt tcgcgatctg
     8761 ccgaacttag cggtgcggta tggcgcaaaa tcatcagcgt gccgctggtt gcctgtacca
     8821 gttcaacatg accaagatgg cgcatagctt gtaagctgcc caggcatgcc ctgactttgg
     8881 gcagcaatgc ttcaagttgg ggcactaaaa tggggcattg tttaacgtcg acaatgtcac
     8941 tggagcccgc tttgcgaaag cccatctgaa gttgctgtgt tttcggtaag tagttcaaac
     9001 ttaaacgcgc gcgacggcga tagccccagg gaacatcggc gatcacttca gagacctcgt
     9061 gtttcattaa tcgggcgagt gccgcacttt tgcttcgctg ctgtaaatcc tcgctggcgt
     9121 gttgttgctg acagccgccg catacgccaa aatgagggca gcgtggcgtt tcgcgttccg
     9181 ggctatcgct taaccggcgt acgactttag cgcgggcgta ttgtttttta tcttcagtaa
     9241 cagtaacttc cgcgttttcc tgtggcaata atccaggaat aaataacgct ttgccgttat
     9301 gtcgtgccac gccctgacca aaagagtcga ggtcgttgac tgaaacagtt atgatctgac
     9361 gcgtcgtcgt gcgtcgtttt gcagagtaga attgcgccat tggcgagact ttctcaattt
     9421 aacagtgtga ccttaattgt cccataacgg aactccatga ccaactacag cctgcgcgca
     9481 cgcatgatga ttctgatcct ggcaccgacc gtccttattg gtttattgct gagtatcttt
     9541 ttcgttgtgc atcgctataa cgacttgcag cgtcaactgg aagatgccgg agccagcatt
     9601 attgagccgc ttgcagtttc tactgaatat ggcatgagcc tgcaaaatcg cgaatctatc
     9661 ggtcagttaa taagcgtact gcatcgtcgc cattccgata ttgttcgcgc gatttcggtt
     9721 tatgatgaaa ataaccgact ctttgtcacc tccaattttc atcttgatcc ctcatcaatg
     9781 cagctcggca gcaacgtgcc gtttcctcgc cagctcactg tcactcgtga cggcgatatt
     9841 atgatcctcc gcacgccgat tatttctgag agttactccc ccgacgaatc gcccagtagc
     9901 gatgccaaaa atagtcaaaa tatgttggga tatattgcgc ttgagctgga tcttaaatcg
     9961 gttcgcttgc agcaatataa agagatcttt atttccagcg tgatgatgct gttttgtatc
    10021 ggtattgcgc ttatttttgg ctggcgctta atgcgcgatg taaccggtcc gattcgcaac
    10081 atggtgaata ccgtcgaccg catccgtcgc gggcaactcg acagccgggt ggaaggattt
    10141 atgctcggcg agctggatat gctgaaaaac ggtatcaact cgatggcaat gtcgctggct
    10201 gcttatcacg aagagatgca gcacaatatc gaccaggcga cgtccgatct gcgtgaaacg
    10261 ctggagcaga tggaaattca gaacgttgag ttggatctgg cgaaaaagcg tgcccaggaa
    10321 gcggcgcgta ttaaatccga gtttctggca aatatgtcac acgagctgcg tacaccactg
    10381 aatggtgtta ttggctttac tcgcctgacg ctgaaaacag aattaacacc aacgcagcgc
    10441 gatcacctga atacgattga acgttcggca aataatttgc tggcaattat taatgatgtt
    10501 ctcgacttct cgaaactgga agcaggtaag ctgattctgg aaagtattcc attcccacta
    10561 cgcagcacgc tggatgaagt cgttaccctg ctggcacatt cttctcacga taaagggctg
    10621 gaactgacgc tcaatattaa gagcgacgtg cccgataacg taattggcga cccactgcga
    10681 ttacagcaaa tcatcactaa cctggtaggg aatgcaatta aattcaccga gaatggcaac
    10741 attgatattc tggtagaaaa acgtgcgctg agtaatacca aagtgcagat tgaagtgcag
    10801 attcgggata ccggcattgg tattcctgaa cgcgatcaat cgcgcttatt ccaggccttc
    10861 cgacaggctg atgccagtat ttcccgccgt catggtggca ccgggctggg gctggtgatt
    10921 acacaaaaac tggttaatga aatgggcggc gatatttcgt tccatagcca gccgaatcgc
    10981 ggttcaactt tctggttcca cattaatctc gatctgaacc cgaacattat tatcgaaggg
    11041 ccatccaccc agtgcctagc cggcaagcgc ctggcctatg tcgaaccaaa ctccgcagca
    11101 gcgcaatgca cgctggatat tttaagtgaa acgccgctgg aagtggttta tagcccaacg
    11161 ttctccgcgc tgcctcccgc gcattacgac atgatgttat taggcattgc ggtgaccttc
    11221 cgcgagccgc taacaatgca acatgagcga ttagcgaaag cggtatcgat gaccgatttt
    11281 ctgatgctgg cacttccttg ccatgcacaa gtcaatgctg aaaaacttaa gcaagatggt
    11341 atcggcgcgt gtctgctgaa accattaaca cctacgcgcc tgttacctgc cctgacggaa
    11401 ttttgtcatc acaaacaaaa cacgcttttg cctgtaaccg atgaaagtaa gctggcaatg
    11461 acagtcatgg cggttgatga caaccccgct aacctgaaac ttatcggcgc attgctggaa
    11521 gatatggtgc aacatgtgga actttgcgat agcgggcatc aggcggttga acgggcgaaa
    11581 cagatgccgt tcgatttgat cttaatggat attcaaatgc cggacatgga cggcattcgg
    11641 gcctgcgaac tcatccacca gctcccccat cagcaacaaa cgccagttat cgcggtaacg
    11701 gcgcatgcaa tggccgggca aaaagagaag ctgcttggcg cagggatgag cgattatctg
    11761 gcgaaaccga ttgaagaaga gcgattgcat aatttgttgt tgcgctacaa gcctggcagc
    11821 ggtatttcgt ctcgcgtcgt gacgcccgaa gtcaacgaaa ttgtggtgaa cccgaatgcg
    11881 accctcgact ggcaactggc actacgccag gcagcaggaa aaaccgattt agcgcgcgat
    11941 atgctgcaaa tgttactcga tttcctgcct gaagttcgca acaaagttga ggaacagctg
    12001 gttggagaaa acccggaagg cctggttgat ttgattcata aactgcatgg cagttgcggc
    12061 tatagcggtg tgccgcgtat gaagaatctc tgccagctga tcgaacaaca gctacgtagc
    12121 ggtactaaag aagaagattt ggagccggag ctgctggaac tgttggacga gatggataat
    12181 gtcgcgcgcg aagccagcaa aattctcggg taatggacgt cttgtcgagt caaaataggt
    12241 tccgtccaat gccaatgctg ggagcagcac agacagtccc ctcgcccctc cggggagagg
    12301 gttagggtga ggggaacagg ccaacgcaag tgcgaatgat taccctcacc ccgcccctct
    12361 ccctgaaagg gcgaggggga aaaccggccc cgtaaaccac ttcgtcagta atctcaaacc
    12421 gacttcttat gaagccggtt ttggttttct gtcttaacgc accatgcacg ggcgcttgtt
    12481 atcaaacgtc cagccaggaa tcaggtactg cattcccatc gcatcgtcac gcgcaccaag
    12541 cccatgtttc tgatacagct cgtgggcttt catcacctga tccatatcga tttctacgcc
    12601 caatcctggt ttttgcggca cctgtaccag cccgcctttg atctcaaacg gttctttggt
    12661 cagacgctga ttgccttcct gccagatcca gtgcgtatca atagcagtaa ttttacccgg
    12721 tgcagcagcg gctacatggg taaacatcgc cagggaaata tcgaagtggt tgttagagtg
    12781 tgaaccccag gtcagcccaa attcatggca catttgcgcc acacgtaccg aaccttgcat
    12841 cgtccagaaa tgcggatccg ccagcgggat atcaacggat tgtaacgaga gcgtatggcc
    12901 catctgccgc cagtcggtgg cgatcatatt ggttgcggtc ggcaggccag tcgcgcgacg
    12961 gaactccgcc atcacttcac gcccggagaa accttgctcc gcaccacacg gatcttctgc
    13021 ataagccagc gaccctttca ggtatttacc aattttaatc gcttcgttca gcgaccaggc
    13081 accgttagga tcgagcgtaa tacgcgcctg cgggaaacgt ttcgccagtg ccacaataga
    13141 ctcggcttct tcttccccgg ccagtacacc gcctttcagt ttgaaatcgt tgaagccata
    13201 tttttcatac gccgcttccg ccaggcgcac caccgcatcc ggcgtcatcg cttcttcgtg
    13261 acgcaggcga taccagtcac atttatcatc cggctggctt tgatacggca gcggtgtggc
    13321 tttgcgatta ccgacgaaga acagataacc gagcatttcg acttcgctac gctgttgacc
    13381 atcgcccagc agggatgcca cgttaacccc cagatgctgc cccagcagat ccagcattgc
    13441 cgcttctatc ccggtaacca catgaatagt ggtccgcagg tcaaatgtct gcaaaccgcg
    13501 cccgccggca tcacgatcgg caaatgtatt acgtaccagc gtcagaacgt ttttgtattc
    13561 acccagcgtt ttacccacca ccagcggaat cgcatcttcc agcgttttac ggattttctc
    13621 gccgccggga atttccccta cgccagtgtg accggaatta tctttgataa tcacaatatt
    13681 acgcgtgaag aacggtgcat gtgcaccact cagattcatc agcatactgt catgacccgc
    13741 caccgggatg acctgcattt cagtaacaac aggcgtcgta aattgagaac tcatatttat
    13801 gtcctttttc agaatcaatg acggccgaaa acgggacgtt tacggtcaaa ggtccagccg
    13861 gggatcaggt actgcatcgg acctgcgtcg ttacgcgcac cgccaggcag acgtttatag
    13921 gcctcatgtg ccttttgtac ctgttcccag tccagttcca cgcccagacc gggcgcatca
    13981 ggaactgcaa tttttccgtt tttaatctcc agcggatttt gggtcaggcg acaatctccc
    14041 tcctgccaaa tccagtgggt atcgatagcg gtaggattac ccggtgccgc cgcacccaca
    14101 tgggtaaaca tcgccagaga gatatcaaag tggttgttgg aatgacagcc ccaggtcagc
    14161 ccccagtcgt cgcaaagctg cgccacgcgg actgcaccgg aaagcgtcca gaagtgcgga
    14221 tcggcaagtg gaatatctac tgcattgagc attaccgcat gacccatttc acgccagttg
    14281 gtggcgatca tgttagtcgc gacgggcaag ccggtcgccc gccgaaactc cgccatcact
    14341 tcacgaccgg agaaaccctg ttctgcgccg catggatctt cggcataggt aagaacatca
    14401 ttcagccctt tgcacaaaga aatggcttca tcaagcagcc atgcaccgtt gggatcaacg
    14461 gtaatccgcg catccgggaa gcgtttcttc aatgcacgaa cagtgtcgat ttcttgctcg
    14521 ccaggtaaca ctccgccctt aagtttgaaa tctttaaagc cataacgatc ctgtgaagct
    14581 tccgccagac gcacaacggc ttcgctgttc atcgctttct gatggcgcaa ctgataccac
    14641 tcatggttgc ccggcgtatt ttccagataa ggaagatcgg ttttggtccg atcaccgata
    14701 taaaacagat aaccgaggac ggtaatagcc tcgcgttgct tgcctggccc caacagttcg
    14761 cagaccggaa cattcagcgc cttacctagc aggtcaagca aggcggcttc cagcgccgcc
    14821 acggcattaa cgcgcaattc aaaagtccag gcacctttgc cgaaggtatc aaaatcggct
    14881 gcctgattac ctttatgcac ctgctgaacc actttattca ggcgcgcaac ttcctggccc
    14941 aacaccatcg gaatagcatc gacaagcgtc tgataaatca cctctccgcc tggcgcttca
    15001 ccaatgccgg tatgcccggc gttatcggtg agtaccacaa tattgcgagt gaaatatgcg
    15061 ttatgcgccc caccaatatt aagcaacatg ctatcgtgcc cggcgaccgg aatgactttc
    15121 atatcagtaa taacaggact ggattgtgtc gtcatcttat tgccccgcga caggtttcag
    15181 ctcgatacgc ttgatatcgc ccaccagcac caggtagctc agtaccgcga ttaaggcatg
    15241 aacaccaaca taaatcagcg ccccattaaa cgagccagtc gtgccaacga tataaccaat
    15301 tgcgattggc gtgacgatgc cagaaatgtt accgaacatg ttgaacaggc caccggaaag
    15361 accgctgatc tcttttggcg cggtatctgc cattactgcc caacccagcg ccccgatgcc
    15421 cttaccgaag aaggccagcg ccataaagcc gatgatcatc cactcaacgt tgacgtagtt
    15481 gcagaacacc atcaccatcg acagcaacat gcccattacg atcggtgttt tacgcgcaat
    15541 gttcagcgat cccgtgcggc gcatcagcca gtcggaaata atcccaccca gcacaccgcc
    15601 gataaaaccg caaaccgccg gaacggaagc cacaaagccc gctttcagaa tcgacatccc
    15661 gcgtgcctgc accagataaa ccgggaacca ggtaataaag aagtaagtca gggcgttgat
    15721 acagtactga ccgatataaa cgccgatcat catccgtgac cctagcagct gtttgatctg
    15781 cccccacttc acgctgaacg gtactttaac tttggtgttt tgctgatcca tattgatcag
    15841 cgcgccaccc gcggcgatgt actccagctc tttctgattt acacccggat gttgatttgg
    15901 ctcgtggatg actttcagcc agaaaaagct gatgacaatg cccagaccgc ccataaagaa
    15961 gaagacgtgt gaccagccca cttcatgagt cagccagccc ataatcggcg cgaagatcac
    16021 cgttgcgaag tattgagcgg agttaaaaat agacaccgcc gttccccttt cctgcgccgg
    16081 aaaccaggcc gcaacaatgc gactgttgcc ggggaaagat ggcgcttcag caagcccgac
    16141 caggaagcgc agcgtaaaca gggcaacgat aatgccgaat ccactaaaga tatcgacgaa
    16201 gccttgcagc aaggtaaaca tcgaccagat aaagatcgac cagaagtaga cgcgttttga
    16261 accaaaacga tccagcaacc aaccacctgg gatctgcccg ataacataag cccatgagaa
    16321 agcagagaac acatagccca ttcccacagg atcaaggccg atatctttgg ccatttccga
    16381 accggcgata gagagcgtag cgcggtcgcc gtagttgaag gatgtgacga taaacaacat
    16441 caccactatc cagtaacgag catttgtgcg tttttccaca ctgctcgcag cctgacttaa
    16501 agaactcatt gttgcactcc tgaaaattcg cgttagccac gctcactctg gactgcgaca
    16561 tcgccaggaa atcagaggtg acgtagggtg ttttttgccg tttttatagg tcgttcgccg
    16621 aatacggcgc gtgtttatat ctggcaatag cagtataaaa agcgcgccat agcggctcac
    16681 cgtgcaacaa cacaacatta atgcgttcaa tgaggcccga ttttggcatt agccctggac
    16741 ggtggaatcc acttcacgga aatgaaaaca agaacaagaa aggaagggtt aaaacgaaga
    16801 aataaagaag agtatgaaat ggatcgcttg actccaggca aacgccagta aaaatccgcg
    16861 ctatgaagca gtttttactg gcatttgcct gaaaagattc gattcagcac cgctaaaacg
    16921 gcatttaccg ctcgctgaac gtatcaggac agcagcgtgc cccaatgttc gacccacgga
    16981 tttgattccg tttccggttc cgggttctca ctggcgtcaa tcaacagcat ttcgccaacc
    17041 cgctgagcgc tctgttcctg caacaaggca tcgaactgtt tgccaccatt gcagaaattc
    17101 acataactac tgtcgccgag cgcaatcacg ccataacgca gattcggctg gaagcccagg
    17161 gtatctttga ttccctgaaa gagcggcaca atgctatcag gaaggtcgcc ctgcccggtc
    17221 gtggacgtaa ccaccagaac atacttatcc tgatagggca gccagtcgct taattcagga
    17281 tcttcaaata ccgttgcttt gtggccctgc gcggtcagaa ttgcttccgc ttcttccgcc
    17341 actaacagcg aatttccgta catagtgccg acaaaaatac caatttccgc cataccgttt
    17401 tctccctgga ttaggaactt atctctccat cctgacccga tggcgcactg aactcaaccc
    17461 tttcattttc aggaagcaga ccccgccagc caaattgtga taacgcctgc atccaggtgt
    17521 cgtccaggcc cgcgtgaata gtcaacggct cgccagtaaa aggatgcgtc aatgacaact
    17581 gactggcatg cagcattaac cgctgaaggc caaaatgttc agcaccgctg cgattctggc
    17641 gtaaatcacc atgtttgctg tcgccaataa tcgggtggcg caaatgggca agatggcgac
    17701 ggagctggtg cttacgtccg gttttcggct ccagttccac caggccgtag cgcgtggtcg
    17761 ggtaacgtcc ggtcgctacc ggcatttcta cggtcgccag accgcgataa tgcgtcactg
    17821 ctggctgtgg gcctttatct tcgcgggcaa atttatcagc gattttgtcc agttcttcca
    17881 ccagtggata atccagaact gcttcttcca tcaaccagcc gcgcacaatc gcatggtaac
    17941 gtttctggat ttggtgctgt tcaaactgtt gtgccagcag ccgtccggcc tcgctggata
    18001 atcccatcaa caacacacca gaagtgggtc ggtccagacg atgagcagta aacacatgct
    18061 ggcctatctg gtcacgcacg gtttgcatga ccactacttt ctcgtcgcga tccagccagc
    18121 tacggtgaac cagccagccg gagggtttat ttaccgcaac cagccattca tcctgataga
    18181 gtatttccag cattagctcg catcatccgc aaaaagagca tccagttttt ccagctcagc
    18241 cagaataagc gcgcgttgcg ggtggtccgt cgccagcgcc atttcataat agggagcaac
    18301 ggcaaaagcg cccggtaacg gctggttgtt ttctaacaaa tcgtgcattc gcgggatcag
    18361 cacccactgc aaccactcca gtggttccat ggtgtccata aagaacggtt gggtactgtt
    18421 aaattgatgc ggctggggtt catcgttttg ccagtgctga tgttcacgca gtaatgcttc
    18481 aagcgcctgc agctggagac gaacgcggtc atgagtggtc ataaagcaac ctcaataaga
    18541 aaaactgccg cgaaggatag caggaataaa aaaagggagc actgtattca cagtgctccc
    18601 ggttcgtttc gcagcattcc agctactttt gttgctccct gctcatcctt gacaactttt
    18661 cctctggcct tgcggccaat cgttcatcct gaactattgc ttcctgctca caccaccccg
    18721 atgtgatact tcatcctgaa gtgtccctgg ccttcctgac ccaccgaatc atcctgaccg
    18781 gttctcattc tccatcctgg aggtgtcctt taacgcgtcc tgcgtcatcc tcttcgcttc
    18841 atccagaagc ctttccctga aacaccatcc tggtgtgtcc ttcagaagtg tcatcatcct
    18901 gatgttcact tcgttgtctg actccctgtc gacgaagata gaatcgtctt tttcgcgaag
    18961 tcttacaagg cgcttacaga caatgcctta aggaaaattt catatgaaag ttaaggattt
    19021 acaagcaata tattgatttt aaatgaaaat aacgattctt atctttttaa gtaatcgctt
    19081 atttcctggc gatctcctac agagtctgta agagatctct cacaaccaga tggggtattt
    19141 ggattacaga agaggcttaa gttgattaag gaattccgca agatttgagg ctaaatgcgc
    19201 ccgtttacgt gttcccagcg tctctttaca cacttcaccg gagagattac acaccgagat
    19261 cacctctaac tcttcttcca gcgtcgcgat aaatagcgtg ggcggcaatt ttagcctttt
    19321 ctgagtcacc agatgaccaa tcagattttc ctgcacccga cggaagtcgt cttcactcca
    19381 ggtttgcagc aatgtcagtt taatatcgcc aaactgcgcg tgcatatccc cggcaaactg
    19441 agtcgtataa aaggtatgaa ttgtgggttg tatcacaata tcaaaagcgc gttcaaccgc
    19501 gtttacattt tgttccccgg taaacggctg gggttgccag tatacggcgt cttcggtggt
    19561 tgaaataatg cacggtgaag gaacgccgta cagttcctca ctcaacggcc agcttttatg
    19621 ctcttcatgc cacgcatcgc agtagcgtgc agtaaaatct ttcagggctt gtgcggtcaa
    19681 atcgtccact aatttctctc ttcacttaaa ccagatacac ttgtcgttta gtttatctgg
    19741 tttatgacgg tgaaacatgt cttcttatgc aaaccatcag gcacttgcgg gcctgactct
    19801 tggaaaatca accgattacc gggataccta tgacgccagc ctactgcaag gcgttccgcg
    19861 tagtctgaat cgcgatccgc tgggtctgaa agcggataac ctgccttttc acggtacgga
    19921 tatctggacg ctatatgaac tttcctggct gaatgcgaaa ggtttgccgc aggtcgccgt
    19981 cggtcatgtt gaacttgatt acaccagcgc aaatctgatt gagtcgaaga gttttaagct
    20041 ctatctcaac agttttaacc agacgcgttt taataactgg gatgaggtgc gccagacgct
    20101 ggagcgcgac ttaagcacct gcgctcaggg taaggttagc gtggcgttat atcgtcttga
    20161 tgaactggaa ggccaaccga taggtcattt caatggcact tgcattgatg accaggatat
    20221 cactatcgat aactatgaat tcactactga ctatctggag aatgccacca gtggtgaaaa
    20281 agtagtggaa gagacgcttg tcagccacct gctgaaatca aactgcctga tcacccatca
    20341 accagattgg ggttcgatcc aaattcagta tcgtggacgc caaattgaca gagaaaaact
    20401 gctgcgttac ctggtttctt tccgccatca caacgagttc cacgaacagt gcgtggaacg
    20461 catctttaat gacctgttac gcttctgcca gccagagaaa ttgagcgttt acgcacgtta
    20521 tacccgtcgt ggcggtctgg acattaaccc gtggcgcagt aatagcgatt ttgtcccatc
    20581 gaccacaaga ctggttcggc aataaatttt ttctcaattt tgcgtgctgg attcacgcag
    20641 aaggttgtga aaggtcatca ggcagggcta ttgtaatcaa agggaatgac gatattcgtc
    20701 ccataaggag tttttcttga ttacacatat tagcccgctt ggctccatgg atatgttgtc
    20761 gcagctggaa gtggatatgc ttaaacgcac cgccagcagc gacctctatc aactgtttcg
    20821 caactgttca cttgccgtac tgaactccgg tagcttgacc gataacagca aagaattgct
    20881 gtctcgtttt gaaaatttcg atattaacgt cctgcgccgt gaacgcggcg taaagctgga
    20941 actgattaat cccccggaag aggcttttgt cgatgggcga attattcgcg ctttgcaggc
    21001 caacttgttc gcggttctgc gtgacattct cttcgtttac gggcaaatcc ataacaccgt
    21061 tcgttttccc aacctgaatc tcgacaactc cgtccacatc actaacctgg tcttttccat
    21121 cttgcgtaac gctcgtgcgc tgcatgtcgg tgaagcgcca aatatggtgg tctgctgggg
    21181 cgggcactca attaacgaaa acgaatattt gtatgcccgt cgcgtcggga atcagctggg
    21241 cctgcgtgag ctgaatatct gcaccggctg tggtccggga gcgatggaag cgccaatgaa
    21301 aggtgctgcg gtcggacacg cacagcagcg ttacaaagac agtcgtttta ttggtatgac
    21361 agagccgtcg attatcgccg ctgaaccgcc taacccgctg gtcaacgaat tgatcatcat
    21421 gccggatatc gaaaaacgtc tggaagcgtt tgtccgtatc gctcacggta tcattatctt
    21481 ccctggcggt gtgggtacgg cagaagagtt gctgtatttg ctgggaattt taatgaaccc
    21541 ggccaacaaa gatcaggttt taccattgat cctcaccggc ccgaaagaga gcgccgacta
    21601 cttccgcgta ctggacgagt ttgtcgtgca tacgttgggt gaaaacgcgc gccgccatta
    21661 ccgcatcatc attgatgacg ccgctgaagt cgctcgtcag atgaaaaaat cgatgccgct
    21721 ggtgaaagaa aatcgccgcg acacaggcga tgcctacagc tttaactggt caatgcgcat
    21781 tgcgccagat ttgcaaatgc cgtttgagcc ctctcacgag aatatggcta atctgaagct
    21841 ttacccggat caacccgttg aagtgctggc tgccgacctg cgccgtgcgt tctccggtat
    21901 tgtggcgggt aacgtaaaag aagtcggtat tcgcgccatt gaagagtttg gtccttataa
    21961 aatcaacggc gataaagaga ttatgcgtcg tatggatgac ctgctacagg gttttgttgc
    22021 ccagcatcgt atgaagttgc caggctcagc ctacatccct tgctacgaaa tctgcacgta
    22081 atctccctcc cccggtgacg tttgttgccg ggttttctcg tttttggtca catacccatc
    22141 aatttatttc atttgttata tgaatgtttc ttaccaccct cacgcgataa atatcattac
    22201 agttaatatg tcatacaatt tatgtcgtaa cgcaaacgtt tccctatttt cataaaccac
    22261 attttatcgc tacgaaaaag aaattatcgc catgattaac taaaagtatt gattttttca
    22321 gttcaaccta catatattgc gcgccccgga agaagtcaga tgtcgtttaa tgggcaaata
    22381 ttgcccttaa attctctttt acttttgatt tacagagtaa agcgttggga taatctatct
    22441 tccaagtaga ttattgtatt tgggatcaag atcactgata gatacataac ttgtgtgtat
    22501 ctttccgccc tcaaattatt acggcggtaa atgattaagc catcgccgat agacagattt
    22561 catttttacg gtcaggcacc ttcccgggct gaactggcta aaagctgaat tatttgcatt
    22621 cctccaggag aaatagatgg aaacgactca aaccagcacg attgcgtcga aagactctcg
    22681 tagtgcctgg cgcaagacag acaccatgtg gatgctgggc ctttacggca cggcaatcgg
    22741 cgcgggcgtg ctgttcctgc caatcaacgc cggtgttggc ggtatgatcc cgctgatcat
    22801 catggctatc cttgcgttcc cgatgacatt ttttgctcac cgcggcctga ctcgcttcgt
    22861 actgtctggt aaaaacccag gcgaagacat caccgaggtt gtagaagaac actttggtat
    22921 tggcgcaggt aaactgatta ccctgctcta cttcttcgct atctacccga tcctgctggt
    22981 ttatagcgtg gcaatcacca atacagttga aagcttcatg tctcaccagc tgggtatgac
    23041 accaccgccg cgtgcgattc tgtcgctgat cctgatcgtg ggtatgatga ccatcgttcg
    23101 cttcggtgag cagatgatcg ttaaagcgat gagtattctg gtattcccgt ttgttggcgt
    23161 actgatgctg ctggctctgt acctgatccc gcagtggaac ggcgcagcac tggaaacgct
    23221 gtctctggac actgcatcgg caaccggaaa cggtctgtgg atgaccctgt ggctggcaat
    23281 tccggtaatg gtgttctcgt tcaaccactc tccgatcatc tcttctttcg ccgttgcgaa
    23341 gcgtgaagag tacggcgata tggcagaaca gaaatgttcg aagatcctgg cattcgcaca
    23401 catcatgatg gtgctgaccg taatgttctt cgtcttcagc tgcgtactga gcctgactcc
    23461 ggcagacctg gctgcggcta aagagcagaa catctcgatt ctgtcttacc tggctaacca
    23521 ctttaacgca ccggttatcg cgtggatggc tccgattatc gcgattatcg ctatcaccaa
    23581 atccttcctc ggccactacc tgggcgcacg tgaaggcttc aacggtatgg tgattaaatc
    23641 tctgcgtggt aaaggtaagt ctatcgaaat caacaagctg aaccgtatca ctgcgctgtt
    23701 catgctggta acgacctgga ttgttgccac cctgaacccg agcatcctgg gtatgattga
    23761 aaccctgggc ggcccaatca tcgcgatgat cctgttcctg atgccgatgt acgcaattca
    23821 gaaagtaccg gcaatgcgta agtacagcgg tcacatcagc aacgtattcg ttgtcgtgat
    23881 gggtctgatt gcaatctccg caatcttcta ctctctgttc aactaagtcc tttcgcgccg
    23941 ctttcgggcg gcgcttcctc cgttttaacg cgatgtattt cctatgatta gcgtattcga
    24001 tattttcaaa atcggcattg gcccttccag ttctcatacc gttggaccaa tgaaagcggg
    24061 taaacaattt accgacgatc tgattgcccg taacctgctt aaagacgtga cccgcgtggt
    24121 ggttgacgtg tacggctcgc tctctctgac tggtaaaggc caccacactg atatcgccat
    24181 tattatgggc ctggcgggta acctgccgga taccgtggat atcgattcca tccctggttt
    24241 tattcaggat gtgaatactc atggtcgcct gatgctggca aatggtcagc atgaagtgga
    24301 gttcccggtt gatcagtgca tgaacttcca tgccgacaac ctttctctgc atgaaaacgg
    24361 tatgcgcatt accgcgctgg cgggcgataa agtcgtttac agccagactt attactctat
    24421 cggcggtggc tttattgttg atgaagaaca ttttggtcag caggatagcg caccggttga
    24481 agttccttat ccgtacagtt cagctgccga tctgcaaaaa cattgtcagg ataccgggct
    24541 gtcactctct ggcctgatga tgaaaaacga gctggcgctg cacagcaaag aagagctgga
    24601 acagcacctg gccaacgtct gggaagtcat gcgtggcggt attgagcgcg gtatttccac
    24661 cgaaggcgtg ttgcctggca aactgcgcgt tccacgccgt gctgcggcac tacgccggat
    24721 gctggtcagc caggataaaa ccaccactga cccgatggcg gttgttgact ggatcaacat
    24781 gtttgcactg gcagtgaacg aagagaacgc tgctggcggt cgcgtggtga ctgcgccgac
    24841 taacggtgcg tgcgggatta tcccggctgt gctggcgtac tacgataagt ttatccgcga
    24901 agtgaacgct aactcactgg ctcgttacct gctggtagcc agcgccattg gttctcttta
    24961 taagatgaac gcgtcgattt ctggtgccga agtgggttgc cagggtgaag ttggcgtggc
    25021 gtgctctatg gcggcagctg gtctggcaga actgttaggc gcaagcccgg cgcaggtgtg
    25081 catcgcggcg gaaatcgcca tggagcataa cctcggtctg acgtgtgacc cggttgctgg
    25141 acaggtgcag gtaccgtgca tcgagcgtaa cgccattgcg gcagtaaaag cggtgaacgc
    25201 cgcgcgtatg gcgctgcgcc gtaccagcga gccgcgcgtc tgcctcgata aagttatcga
    25261 gaccatgtac gaaacaggta aagatatgaa cgccaagtac cgcgaaacct ctcgcggtgg
    25321 cctggcaatg aagatcgttg cctgcgatta atctctcccg gaggcctcgt tttgcgaggc
    25381 ctcttcccgt tttctcaacc agccatagcc tgttccggca tcgaatgtta cccttatcgc
    25441 ctgatcttta agggggttat cgtggctgtt catttgctta ttgtcgatgc actgaatctt
    25501 attcgtcgca ttcatgccgt tcagggttcg ccctgtgtcg aaacctgcca gcacgcgctc
    25561 gatcagctca ttatgcacag ccaaccaacc cacgcggtcg ccgtttttga tgatgaaaac
    25621 cgcaatagcg gctggcgtca tcagcgttta ccagattaca aagcgggtcg accgccaatg
    25681 ccggaagagt tgcacgacga gatgcctgca ctacgcaccg cctttgagca acgcggcgtc
    25741 ccgtgctggt cagccagcgg taacgaagcc gatgacttag ccgccacgct ggcggtcaaa
    25801 gtgacacagg ccgggcatca ggcaacgatt gtttcgacag ataaaggcta ctgtcagtta
    25861 ctttcaccga cattacgtat tcgtgattac ttccagaaac gttggctgga tgcgccattt
    25921 atcgataaag aatttggcgt tcaaccgcag cagttgcccg attactgggg gcttgcgggg
    25981 atcagcagtt caaaggtacc gggtgttgcg gggatcggac ccaaaagcgc cacgcagctg
    26041 ctggtcgagt ttcagagtct ggaagggata tatgagaatc tggatgcggt tgccgaaaag
    26101 tggcgcaaaa aattagaaac ccataaagag atggcgtttc tgtgccgcga tattgcccgc
    26161 ttacaaaccg atttgcatat cgacggcaat ttacagcaat tgcggttggt aaggtaacgg
    26221 cgtgccggat acggcgcaaa cgtcgcatcc ggcattatca catcagcgca tttaccaggc
    26281 ggtatggtaa agctctacaa tatcctcaag cgttgcttca cgcgggttgc caccggtaca
    26341 aacatcatcc agtgccgcct gcgccagtgc cggaatgtct tccttgcgta ccccaacatc
    26401 acgcaaatgt ggcggaatac cgacatcacg gttgagagca aacacagctt caacagcggc
    26461 attacgcgcc tcttccaggc tcataccttc cactttcacg cccataacgc gcgcgatatc
    26521 gcggtacttc tcaccggtaa agtcagcgtt atagcgcatg acatgcggta acaggatggc
    26581 gttcgcgaca ccgtgtggag tgttataaaa cgcgcccagt ggatgcgcca taccatgcac
    26641 caaccctaac ccaacattcg agaagcccat acccgcaaca tactgcccga gcgccatttc
    26701 ttctccggca tccttatcac cagcaaccga tcctcgcagc gccccagcaa tgatttcaat
    26761 cgctttaatg tgcagtgcat cggttagcgc ccacgcgcca cgggtaatat acccctcaat
    26821 agcatgagtg agcgcatcga cacccgtcgc agctttcagc gctggaggca taccatccat
    26881 catgtcagcg tcaataaacg ccacctgcgg gatatcatgc ggatcaacgc aaacaaactt
    26941 gcgccgtttt tcttcgtcag tgatcacgta gttaatggtc acttctgccg cagtgcctgc
    27001 tgtggtgggg attgccagaa tcggtacact gggtttattg gtcggggaaa gtccttccag
    27061 gctacgcaca tcggcaaact ccgggttgtt gctgataatg ccaatcgctt tacaagtatc
    27121 ctgtggagaa ccaccaccaa tagcgatcag gtaatccgcg ccgctattct ggaatacacc
    27181 gagcccttct ttgacgacag taattgttgg gttgggcact acgccgtcgt aaatcgccca
    27241 tgccagccct gcagcatcca tcttatcggt cacttttgcc accacgccgc attgcaccag
    27301 cgttttatcg gtgacgatca gcgccttctg ataaccacgg cgtttcacct catcggttaa
    27361 agccccaaca gcaccccgac caaaccatgc cgtttcgttc agaatcattc tgttagccat
    27421 catccttctc cttgttgctt tacgaaatta ctcttcaatc cgtaacccat aggttttgaa
    27481 tttctccagc actacggcaa tctcttcatc gctcagcact ggcaccgggt ccgtaatcgc
    27541 cagggtcgtc aggtaaagtt gcgccagcac ttcaacttca tgcgccagcc ataacgcttt
    27601 ttccagattc acctcacaag cgataagccc atgatgttgt aacaaagttg ccttacgatt
    27661 tttgagagcc agcgcaacat gttcagaaag ttcacgtgtt ccaaaggtcg cataaggcgc
    27721 gcaaggaata gaattaccgc cagccgccgc aatcatgtag tgaatagcgg ggattggtcg
    27781 gttaagaatg gaaactgccg tgcaatgaac ggcatgattg tgaacaaccg cgttggcatc
    27841 cggtctgctt tgataggccg ccatatggaa acgccattcg cttgagggga gctttccttc
    27901 ctcatgttta ccgttgccat caataaagac aatatgcgac tccgtcagtt tttcatatgg
    27961 aatacctgta ggcgtaatca gcatcccatc ctgataacgt acactgacgt tacccgctgt
    28021 cccctggttc agtcccaggc gggtcatttc caggcaagtg tcaataatct gacgagcaag
    28081 tttatttcgt tccattagct acctctctct gattcaaaac agggcaataa tgttgttcct
    28141 ttcacactat tgaattagcc gtttaattac ccaccatctt cttcctgatt aaccagaaag
    28201 aaattcacaa gcttatattt tgtgacctgg ttcaactaat catagtaaat aaccgcaagt
    28261 tctcttttta taaccctatt aaaaatgacc gctcttaaaa atatttatca aaacggtcat
    28321 ttttccattc cccctcgcca gaaatgcccg ttttcggcac aaacaattaa tacggtcatc
    28381 gggtttgtgt tttttatgat ttattttctg aaacgggcat gaaatttcga ttattaaagt
    28441 gatggtagtc acataaagtc acctcccagc taataagtgt gaccgccgtc atattacaga
    28501 gcgtttttta tttgaaaatg aatccatgag ttcatttctg acaggcaaat attcactgat
    28561 atgaagcccg aactcgctgg ttttgcactt ttgaaaacat aaccgattac gtgcttaagc
    28621 ttctgaacct aagaggatgc tatgggaaac acatcaatac aaacgcagag ttaccgtgcg
    28681 gtagataaag atgcagggca aagcagaagt tacattattc cattcgcgct gctgtgctca
    28741 ctgttttttc tttgggcggt agccaataac cttaacgaca ttttattacc tcaattccag
    28801 caggctttta cgctgacaaa tttccaggct ggcctgatcc aatcggcctt ttactttggt
    28861 tatttcatta tcccaatccc tgctgggata ttgatgaaaa aactcagtta taaagcaggg
    28921 attattaccg gattattttt atatgccttg ggcgctgcat tattctggcc cgctgcagaa
    28981 ataatgaact acaccttatt tttagttggc ctatttatta ttgcagccgg attaggttgt
    29041 ctggaaactg ccgcaaaccc ttttgttacg gtattagggc cggaaagcag tggtcacttc
    29101 cgcttaaatc ttgcgcaaac atttaactcg tttggcgcaa ttatcgcggt tgtctttgga
    29161 caaagtctta ttttgtctaa cgtgccacat caatcgcaag acgttctcga taaaatgtct
    29221 ccagagcaat tgagtgcgta taaacacagc ctggtattat cggtacagac accttatatg
    29281 atcatcgtgg ctatcgtgtt actggtcgcc ctgctgatca tgctgacgaa attcccggca
    29341 ttgcagagtg ataatcacag tgacgccaaa caaggatcgt tctccgcatc gctttctcgt
    29401 ctggcgcgta ttcgtcactg gcgctgggcg gtattagcgc aattctgcta tgtcggcgcg
    29461 caaacggcct gctggagcta tttgattcgc tacgctgtag aagaaattcc aggtatgacc
    29521 gcaggctttg ccgctaacta tttaaccgga accatggtgt gcttctttat tggtcgtttc
    29581 accggtacct ggctcatcag tcgcttcgca ccacacaaag tcctggcagc ctacgcatta
    29641 atcgctatgg cactgtgcct gatctcagcc ttcgctggcg gtcatgtggg cttaatagcc
    29701 ctgactttat gcagtgcctt tatgtcgatt cagtacccaa caatcttctc gctgggcatt
    29761 aaaaatctcg gccaggacac caaatacggt tcgtccttca tcgttatgac cattattggc
    29821 ggcggtattg tcactccggt catggggttt gtcagtgatg cagcgggcaa catccccact
    29881 gccgaactga tcccggcact ctgcttcgca gtcatcttta tctttgcccg tttccgttct
    29941 caaacggcaa ctaactgaac atattttccg aataaagtga ggaatctgta atgaaaaaaa
    30001 tcagcttacc gaaaattggt atccgcccgg ttattgacgg tcgtcgcatg ggtgttcgtg
    30061 agtcgcttga agaacaaaca atgaatatgg cgaaagctac ggccgcactg ctgaccgaga
    30121 aactgcgcca tgcctgcgga gctgccgtcg agtgtgtcat ttccgatacc tgtatcgcgg
    30181 gtatggctga agccgctgct tgcgaagaaa aattcagcag ccagaatgta ggcctcacca
    30241 ttacggtaac gccttgctgg tgctatggca gtgaaaccat cgacatggat ccaacccgcc
    30301 cgaaagccat ttggggcttt aacggcactg aacgtcccgg cgctgtttac ctggcagcgg
    30361 ctctggcagc tcacagccag aaaggtatcc cggcattctc catttacagt catgacgttc
    30421 aggatgccga tgacacatcg attcctgctg atgttgaaga aaaactgctg cgctttgccc
    30481 gcgccggttt ggccgtcgcc agcatgaaag gtaaaagcta tctgtcgctg ggcggcgttt
    30541 cgatgggtat cgccggttct attgttgatc acaacttctt tgaatcctgg ctgggaatga
    30601 aagtccaggc ggtggatatg accgaactgc gtcgccgtat cgatcagaag atttacgacg
    30661 aagccgaact ggaaatggcg ctggcctggg ctgataaaaa cttccgctat ggcgaagatg
    30721 aaaataacaa acagtatcaa cgcaatgccg agcaaagccg cgcagttctg cgcgaaagtt
    30781 tactgatggc aatgtgtatc cgcgacatga tgcaaggcaa cagcaaactg gccgatattg
    30841 gtcgcgtgga agaatcactt ggctacaacg ccatcgctgc gggcttccag ggacaacgtc
    30901 actggaccga tcaatatccc aatggcgaca ccgccgaagc gatccttaac agttcatttg
    30961 actggaatgg cgtgcgcgaa ccctttgtcg tggcgaccga aaacgacagt cttaacggcg
    31021 tggcaatgct aatgggtcac cagctcaccg gcaccgctca ggtatttgct gatgtgcgta
    31081 cctactggtc accagaagca attgagcgtg taacggggca taaactggac ggactggcag
    31141 aacacggcat catccatttg atcaactccg gttctgctgc gctggacggt tcctgtaaac
    31201 aacgcgacag cgaaggtaac ccgacgatga agccgcactg ggaaatctct cagcaagagg
    31261 ctgacgcttg cctcgccgct accgaatggt gcccggcgat ccacgaatac ttccgtggcg
    31321 gcggttactc ttcccgcttc cttaccgaag gcggcgtccc gttcaccatg actcgtgtca
    31381 acatcatcaa aggcctggga ccggtactgc aaatcgcgga aggctggagc gtggaattgc
    31441 cgaaggatgt gcatgacatc ctcaacaaac gcaccaactc aacctggcca accacctggt
    31501 ttgcaccgcg cctcaccggt aaagggccgt ttacggatgt gtactcggta atggcgaact
    31561 ggggcgctaa ccacggggtt ctaaccatcg gccacgttgg cgcagacttt atcactcttg
    31621 cctccatgct gcgtatcccg gtatgtatgc acaacgttga agagaccaaa gtgtatcgtc
    31681 cttctgcctg ggccgcgcac ggcatggata ttgaaggcca ggattatcgc gcttgccaga
    31741 actacggccc gttgtacaag cgttaataca ttactccctg atgtgatgcc cggtcgctcc
    31801 ggctaccggg cctgaacaag caagagtggt tagccggata agcaatgtta tccggctata
    31861 ttgcaggagc gattatgaaa caagaagtta tcctggtact cgactgtggc gcgaccaatg
    31921 tcaggaccat cgcggttaat cggcagggaa aaattgttgc ccgcgcctca acgcctaatg
    31981 ccagcgatat cgcgatggaa aacaacacct ggcaccagtg gtctttagac gccattttgc
    32041 aacgctttgc tgattgctgt cggcaaatca atagtaaact gactgaatgc cacatccgcg
    32101 gtatcgccgt caccaccttt ggtgtggatg gcgctctggt agataagcaa ggcaatctgc
    32161 tctatccgat cattagctgg aaatgtccgc gaacagcagc ggtaatggac aatattgaac
    32221 ggttgatctc cgcacagcag ttgcaggcta tttctggcgt cggagccttt agtttcaata
    32281 cgttatataa gttggtgtgg ttgaaagaaa atcatccaca actgctggaa cgcgcgcacg
    32341 cctggctgtt tatttcgtcg ctgattaacc accgtttaac cggcgaattc actactgata
    32401 tcacgatggc cggaaccagc cagatgctgg atatccagca acgcgatttc agtccgcaaa
    32461 ttttacaagc caccggtatt ccacgccgac tcttccctcg tctggtggaa gcgggtgaac
    32521 agattggtac gttacagaac agcgccgcag caatgctcgg cttacccgtt ggcataccgg
    32581 tgatttccgc aggtcacgat acccagttcg ccctttttgg cgctggtgcc gaacaaaatg
    32641 aacccgtgct ctcttccggt acatgggaaa ttttaatggt ccgtagcgcc caggttgata
    32701 cttcgctgtt aagtcagtac gccggttcga cctgcgaact ggatagtcag gcagggttgt
    32761 ataacccagg tatgcaatgg ctggcatccg gcgtgctgga atgggtgaga aagctgttct
    32821 ggacggctga aactccctgg caaatgttga ttgaagaggc tcgtctgatc gcgcctggcg
    32881 cggatggcgt gaaaatgcag tgtgatttat tgtcgtgtca gaacgctggc tggcaaggag
    32941 tgacgcttaa taccacgcgg gggcatttct atcgcgcggc gctggaaggg ttaaccgcgc
    33001 aattacagcg caatctacag atgctggaaa aaatcgggca ctttaaagcc tctgaattat
    33061 tgttagtagg tggaggaagt cgcaacacat tgtggaatca gattaaagcc aatatgcttg
    33121 atattccggt aaaagttctc gacgacgccg aaacgaccgt cgcaggagct gcgctgttcg
    33181 gttggtatgg cgtaggggaa tttaacagcc cggaagaagc tcgcgcgcag attcattatc
    33241 agtaccgtta tttctacccg caaactgaac ctgaatttat agaggaagtg tgaaatgctg
    33301 aaaacaattt cgccgttaat ttctcccgaa ctattgaaag tgctggcaga gatgggacat
    33361 ggagatgaaa ttattttttc cgatgctcac tttcccgccc attcgatggg accgcaggtg
    33421 atccgcgctg atggcctgtt ggtgagcgac ttgctccagg cgataatccc gttatttgaa
    33481 ctggacagtt atgcaccgcc gctggtgatg atggcggcgg tagaaggtga cactctcgat
    33541 cctgaagtag aacgacgtta tcgtaatgcg ctttcactac aagccccgtg tcctgacatc
    33601 atccgcatca atcgttttgc gttttatgaa cgggcacaaa aagcctttgc gatcgttatc
    33661 acaggcgaac gagcgaagta cgggaatatt cttttaaaaa aaggggtaac accgtaatct
    33721 cataccggta cgcccgcatg acgcgggcgg ttatcgaatg atggggtgaa aaatatgaaa
    33781 gcggcacgcc agcaagcgat agtcgacctg ctgctgaacc ataccagcct gaccacggaa
    33841 gctctctctg aacagctaaa ggtcagtaaa gaaaccattc gtcgcgatct caatgaatta
    33901 cagacgcagg gtaaaattct gcgcaatcat gggcgcgcta aatatatcca ccgtcaaaat
    33961 caagacagtg gcgatccctt tcacatccgg ctgaaaagcc attatgcgca taaagcagat
    34021 atcgcgcgcg aggcgctcgc gtggattgaa gaagggatgg taatagcctt agacgccagt
    34081 tcaacctgct ggtatctggc acgccagttg cctgacatca acatccaggt cttcaccaat
    34141 agccatccga tttgccatga actcggtaaa cgcgaacgca ttcaactaat cagttccggc
    34201 ggcacacttg agcgcaaata tggctgttac gtcaatccct cgctgatttc ccaacttaaa
    34261 tcgctggaaa tcgatctgtt tattttttct tgtgaaggga tcgatagcag cggcgcactg
    34321 tgggactcca atgccatcaa cgctgattac aaatcaatgc tattaaaacg cgccgcgcaa
    34381 tcgttgttat tgattgataa aagtaaattt aatcgttcag gggaagcccg catcgggcat
    34441 ctggatgagg taacgcacat tatttctgat gagcgccagg ttgcaacttc tttggtaaca
    34501 gcctgacgga aagggtagca ggccggagac gacgccccgg ccttgcctgt tatcgctcgt
    34561 cgcgacgacc acccaccgca gcccagatac ggcggacgtg caccgtcact tcttcgcgat
    34621 cgtgatacaa ctgccgcgcc tgaatctgag catttatgcc atgttcatca agctgtgcct
    34681 gaatatacgc cagattgtgt gacacttctt cgtagcgttt tttcatcggc agtttgaggt
    34741 tgaaaatggt ttcacggcac cagccattaa ccagccactg cgccatcaat gccgcaactt
    34801 tcgccggttt ttcaaccata tcgcatacca tccaggaaat attgctgcgt gtcggacgga
    34861 atttgaaacc atcttcccgc agccacgtca cctgcccggt atccatcaga ctttgcgcca
    34921 tcgggccgtt gtcgacggaa taaacccaca tgttgcgctt caccagttgg taggtccagc
    34981 caccagggca agcgcccaaa tccaccgccc acatcccgtt cgccaggcgt tcatcccact
    35041 catccgcagg aataaacaca tgaaatgcct cttccagttt gagcgtggaa cgactcggcg
    35101 catctgccgg aaatttcagg cgcggaatgc ccatatagaa cggcgaatta ttgttgctgt
    35161 atgagtaacc ggtatagcag cagcctggtg caataaagaa tacatgcaca accggacgct
    35221 tcggcgtttc atagttcgcc agcacacccg catcgcgcaa ggcagcgcgt agcggcacgg
    35281 tgaatttacg gcagaatttc agcaactctt tgctttcgtt ggtatcggca acttcaacac
    35341 gcagttcacc gcccttctct actacgccct gtaacatgcc gacaatggga gtaatacgat
    35401 cttctggcgg caaatgctgc aggagttccc ccaccacaaa ccactggcgg gcaaaaatta
    35461 atgaactgaa cggcagctca cggattaact tatcgccatc atcaggttga taacattcat
    35521 aaatgacata acccgcattc tctttcacgc gggcaaaacc gaaaatttcc cgctggccgg
    35581 ctttatcggt aatttctgcg gcgcactctt tttcaaagcc cggacggcac agcaatacaa
    35641 ccttattcat gacttacgcc cttgcgcttt aaacggatag caccaactaa cattaacgcc
    35701 cagcccgcga ggaagctcac gccgccaacc ggtgtgacaa atgcccacaa acgcagatgg
    35761 gacagcgcca ggcaataaag gctgccgctg aacagcaccg tgcctaacgc gaggaaaacg
    35821 ctactccagt aaaaccagat actgatgcga cgctgcattg ccaccgccag acctaagatc
    35881 gccagcgtat ggaacgcctg atattcgaga ccggtctgga tccagcccat ctcaacggcc
    35941 cccatggttt tacttaacac atgcgcgcca aaagcgccca gagccacaaa aatgaagccg
    36001 ctaatggcgg cgaaaatcag cataaaacgg ctggtcatgg tcgtacccta cgtaaattat
    36061 tgttcataac gaaagcggaa tttttcttgt tcagcagcgg ctttcgccag gatccattgg
    36121 cgaaaggcgg ctattttacc cagttctgcc tggctgtcat gacaaaccag ataaaaagcg
    36181 tttttactga ccagtacatc attaaacggg caaacaagac gtccagcctc aatttcagat
    36241 tgcgccatca cgttatttgc cagcgccact ccctgcccgt ggatagccgc ttgcagcacc
    36301 atggcgctat ggctaaaaat tggcccttgc tgaacgttga tatgatttaa ccccaactgt
    36361 cgggtatatg tctgccagtc acggcgcgac gcatcatgta ataacgtatg tttagccaga
    36421 tcttccgggg tcttcaaggg tttttcgcca gtcagcagta gcggcgaaca caccggcaat
    36481 aaatattcgg cgtacagttt ttccacccgt agccccggcc agttgccccg accataaaat
    36541 atcgccacat caacatcatc cgccagctta tcttcctgac gatcaaccgc ctggattcga
    36601 acgtcaattc ccggataagc tgaattaaag ctggaaagtc gcggaaccaa ccaatgaatg
    36661 gcgaaactgg ggagtaaact gaccgtcaac gcccccttgg cgctacgggc ctggagttta
    36721 cgcgtcgctt cggttaattg cgaaaatatc tctttgatat cgaggaaata gctttgccct
    36781 tcctcggtca gcaggagtga acgattacgg cggcggaaca gttttagccc cagaaaatcc
    36841 tcaagagact tgatttgatg acttactgcg gcctgggtca caaaaagctc ttctgctgcg
    36901 cgagtgaaac ttaaatggcg tgctgcggca tcaaaaactc gtaaggcatt tagcggtggt
    36961 aatcgtttag acatggcttt taaactttga tgttaaatga atttaacaat tagatcacac
    37021 tgtgtaacct attagttttt ttaatctgag ccattataaa ttgtccgttg agcttctacc
    37081 agcaaatacc tatagtggcg gcacttcctg agccggaacg aaaagtttta tcggaatgcg
    37141 tgttctggtg aacttttggc ttacggttgt gatgttgtgt tgttgtgttt gcaattggtc
    37201 tgcgattcag accatggtag caaagctacc ttttttcact tcctgtacat ttaccctgtc
    37261 tgtccatagt gattaatgta gcaccgccta attgcggtgc ttttttttac cttgcgatcg
    37321 cgaattactg atccagttcg accatctctt tcacgtcagt acggttgatc tgctgtttgt
    37381 tgccattagc gtctttatac gaaatcatac cggtatcgtt atcagtctgt ggtttgccgt
    37441 cagagacgat ggtacgtccg tcattggtgt gcatcacata gttcgaaccg gaacaggcgc
    37501 tcagggcaaa agtcagcata caggcagaaa taattgcggc agtctttttc atcatcttct
    37561 ccttaaagcg attaataaat cttaatagta ttgattaaca ggctaaaatt aacgcctaac
    37621 actattcagc atatgttact tacgcggctt cgccaggata tccagataat tctgatggtt
    37681 agcactctcc ttgtatcaaa gtgaattttg cgtcacgatc ggttcatcaa gccgaggagt
    37741 accatgaacg tttttaatcc cgcgcagttt cgcgcccagt ttcccgcact tcaggatgcg
    37801 ggcgtctatc tcgacagcgc cgcgaccgcg cttaaacctg aagccgtggt tgaagccacc
    37861 cgacagtttt acagcctgag cgccggaaac gtccatcgca gccagtttgc cgaagcccaa
    37921 cgcctgaccg cgcgttatga agctgcacga gagaaagtgg cgcaattact gaatgcacca
    37981 gatgacaaaa ctatcgtctg gacgcgcggc accactgaat ccatcaacat ggtggcacaa
    38041 tgctatgcgc gtccacgtct gcaaccgggc gatgagatta ttgtcagcgt ggcagaacac
    38101 cacgccaacc tcgtcccctg gctgatggtc gcccaacaaa cgggagccaa agtggtgaaa
    38161 ttgccgctta atgcgcagcg actgccggat gtcgatttgt tgccagaact gattactccc
    38221 cgtagtcgga ttctggcgtt gggtcagatg tcgaacgtga ctggcggttg cccggatctg
    38281 gcgcgagcga ttacctttgc tcattcagcc gggatggtgg tgatggttga tggtgctcag
    38341 ggggcggtgc atttccctgc ggatgttcag caactggata ttgatttcta tgctttctct
    38401 ggtcacaaac tgtatggccc gacgggtatc ggcgtgctgt atggcaaatc agaactgctg
    38461 gaagcgatgt caccctggct gggtggcggc aaaatgattc acgaagtgag ttttgacggc
    38521 ttcacgactc aatctgcgcc gtggaaactg gaagccggaa cgccaaatgt cgctggtgtc
    38581 ataggattaa gcgcggcgct ggaatggctg gcagattacg atatcaacca ggccgaaagc
    38641 tggagccgta gcttagcaac gctagcggaa gatgcgctgg caaaacgtcc tggcttccgc
    38701 tcatttcgct gccaggattc cagcctgctg gcctttgatt ttgctggcgt tcatcacagc
    38761 gatatggtga cgctgctggc ggagtacggt attgccctgc gggccgggca gcattgcgct
    38821 cagccgctac tggcagaatt aggcgtaacc ggcacactgc gcgcctcttt tgcgccatat
    38881 aatacaaaga gtgatgtgga tgcgctggtg aatgccgttg accgcgcgct ggaattattg
    38941 gtggattaat gacgaacccg caattcgccg gacatccgtt cggcacaacc gtaaccgcag
    39001 aaacgttacg caataccttc gcaccgttga cgcaatggga agataaatat cgccagttga
    39061 tcatgctggg gaaacagctt cctgctttgc cagacgagtt aaaagcgcag gctaaagaga
    39121 ttgccggatg cgaaaaccgc gtctggctgg gatataccat ggctgaaaac ggcaagatgc
    39181 atttctttgg cgacagcgaa gggcgcattg tgcgcggcct gctggcggtg ttgttgactg
    39241 ccgttgaggg gaaaaccgcc gccgaattgc aggcacagtc gccgctggca ttgtttgatg
    39301 agctgggatt gcgtgcgcag cttagcgcct cacgaagcca ggggttgaat gcgttaagcg
    39361 aggcgattat cgctgtggcg aagcaggttt aagcacagaa ccaggtcgga taagacgttc
    39421 gcgtcgcatt cgacctgatt gtttacccct gacgggccgc tttcgccatc atcttcttca
    39481 gcgcatgaga aaccgcaaca aaaccaaagg tggcggtcac catcgtcgcg gcaccaaatc
    39541 cagatgcaca atccatccgc tttggccctt ccgccgtagc tttcattgca cagaccgtgc
    39601 catctgactg cgggtacacc agcgcttcgg tagagaacac gcaatccacg ccgagcttac
    39661 ctttgctgtt tttcactacg ccaaaatcgc ttttcaggcg ctcgcgcaac ttcgccgcca
    39721 gcgggtcctg aatcgttttc gccagatcgg taacctgaat ctgcgtcggg tcaatctgcc
    39781 cacccgcgcc accagtcgtg accagcggga ttttattgcg ccgacaataa gcaatcagcg
    39841 ccgctttggg ccgtacacta tcaatggcat caatcacgta tgaataaccc acgctcatat
    39901 actgcgctac gttatccggc gtcacgaaat catccaccac cgttacacgg cactccgggt
    39961 taatctggcg aatacgctcc gccataactt ccgcttttgc cagcccaacg ttatcgcgca
    40021 gggcatgaat ttgccgattg gtattggtga cgcacacatc atccatatcg ataagcgtga
    40081 ttgcgccaat ccctgtgcgc gccagcgctt ccgccgccca ggaaccgaca ccgccaatac
    40141 cgaccacaca aatatgcgcg tcagcaaaca gttgcaacgc tttttcacca tacagacgcg
    40201 ctgtgccacc aaaacgctga cgccatgcat cactaattac cacagacata gcacctcaga
    40261 ataaaaaggg cgaggttatc cctcaccctg tcgtatccgt aaaaacggca tacagaatat
    40321 cacatcagcc gctaaagacg ttacctgcgc ccggagccgt tttcagcacc cagacacgtc
    40381 catagtggtt gtaccaacct gcgcggtgcc cggcttccgg cccgatccct tgatagatat
    40441 cgaagtgttg gcctttgatt gcgccaccga catccagcgc caccatcaga cgcaattcgt
    40501 actgaccgtt aaatttgccg ttattatcca gcagcggcac ttctgccagc aaggtagtac
    40561 ctggcggaat aattgaacga tcggaggcaa cagaagcacg gccaaccagc ggcaccgcac
    40621 ttgccccttt caccggtgca aaagattgcg gtttaaagaa gacgaaagac gggttctgtt
    40681 ccaacagctc gcgaacctcg gcttcactgt gtgtttcgcc ccagtgacga atcgcctgca
    40741 tcgacatatc ttcttttttc acttcgccac ggtcgatcag caccttacca atgctgcgat
    40801 atgcatgacc gtttttccct gcatagctga aaaagttaag cggactgcca tcaccaaagt
    40861 caatataccc gctaccctgc acatccataa tgaagttatc catcagggag ttgctgtaag
    40921 cgaggatgta tttatcgctc aatgcccccg cgtagatctc cgcacgagac ggcagacgac
    40981 cacgttttgg cggcatacgg taaataggat actggaactc gccctggcgg gtatggcgcg
    41041 cctgaattac cggcgtgtaa tagcctgtaa actgcacgtt accatagttg tcggcacctt
    41101 ccatctgcca ggcatcaatg ccgaactggc gcatattgcg ggtatcaccg cctgcgcgca
    41161 gccactcttg caccgcgtta taaacattac tctggttgcc atacagacgc ggtgacgaat
    41221 tacggatatg gttaatttgc tcggcaaaat caccggcgtt aatcggcgcg ccaacggcat
    41281 ctggctggtt caccagagag aaaggctggg taaatttccc gtctttatat tgctgtccgc
    41341 gatcggttgg tttggaagag caggcggcaa gcattgccac aaccgtgccc ataagaaggt
    41401 actttaccca acgtcctttc attgttctct tcttcaggtt aaaaaataag gcgcaacgaa
    41461 gataacaaac cgccgcgttt aatgaaatga gcagcctctc cctgacgcaa attttgcaca
    41521 aaaaataggc tttagtgatt tgtttttgtt caaaatcatg ccgaatccgt gatcggggta
    41581 aaaaaaggtt gcatgaaaac gcgagcggag tatagtgcgc atccacggac gcggggtgga
    41641 gcagcctggt agctcgtcgg gctcataacc cgaaggtcgt cggttcaaat ccggcccccg
    41701 caaccaatta ttgaacaccc taacgggtgt ttttttgttt ctgttctccc ataaaaaaag
    41761 cgccattcag cgccttttta tcatcccctt ctcgccagcg tcgccccatc ggcaaaatac
    41821 gctttaatcc ccgcaagaat agactccgca acttcctgct ggaaagtcgc cgttttcagt
    41881 ttacgttctt cctcaacgtt actgataaac gccgtttcga ccagaatgga gggaatatct
    41941 ggtgccttta gtacggcaaa cccggcctgt tcaacttgat ttttatgcag cttgttgatt
    42001 ttcccgagct tattcagcac cgctttacca aacttcaggc tgtcggcaat ggtcagcgac
    42061 tgcaccatat cgaacatggt gtggtcgaca tagcggtcac cgcttttgct cacgccacca
    42121 atcaagtccg aggcgttctg ggtttgtgcc agatattttg ccgcagtact ggttgcacct
    42181 ttggttgaga gcgcaaacac agaggaaccg ctcggctgac gactggtaaa ggcgtcggca
    42241 tggatagaga caaacaggtc agcacgctgt ttctgggctt ttgctacgcg cacttgcaac
    42301 ggaatgaaga tgtcttcatt gcgcgtcatg tacaccttca tattgccctc tttctcgatc
    42361 agagagcgca aacggcgagc tatttgcaat accacgtctt tttcgcgcgt tttgtatttc
    42421 cccaccgcac cggagtcttc gccaccgtgg ccagggtcaa gcataatgac aatcggacga
    42481 tcgcgccctg ctttacctgg ttgtggacca ctttgcgctg gcggcacctg cttttcgagg
    42541 tcgcctttgt tgtaatcctc cagcagcgcc agcagcgggt cctgcatatc ctgtgcattg
    42601 gccggataga ggtccatcac cagacgctct ttaaacccgg cgaccggcgc aagggcaaac
    42661 agctgcggtt ttacgttttg ctttaattca aaaaccatgc gtacggtttg cgggtcaaat
    42721 tgcccgacgc gcgccgactt gatgaacggg tcgtctgcac gaatttgtgc cgccatcccc
    42781 ttgagtaccg agttcaggtt tacatcttcg atatccacca ccacgcgttc cgggttactc
    42841 aacgcgaact gcttatattt cagctgacga tttgattcta ccgtcacgcg ggtgtagctg
    42901 gacgcaggcc agacgcgcac cgccacgacc tggctgaccg cagccagact gacctgactt
    42961 acgctcaata gccacatggc acccgcgcct tgcagtaaac gacgacggct gattgcagtg
    43021 ttggatcctg acatgcctct cccgagcaaa agaaatctaa gctgtgtaac aagtaaacga
    43081 ctaatttgac cggaaacttt agcgaaagac gcataatctg tcatctaata aacggtaaac
    43141 attcttttta tattcacggc attactgata aaaaagtcgc tctcgcataa aatttacact
    43201 tgcaccctgc gaaaaaacag aataaaaata cactaatttc gaataatcat gcaaagaggt
    43261 gtgccgtggt aaaggaacgt aaaaccgagc tggtcgaggg attccgccat tcggttccct
    43321 atatcaatac ccaccgggga aaaacgtttg tcatcatgct cggcggtgaa gccattgagc
    43381 atgagaattt ctccagtatc gttaatgata tcgggttgtt gcacagcctc ggcatccgtc
    43441 tggtggtagt ctatggcgca cgtccgcaga tcgacgcaaa tctggctgcg catcaccacg
    43501 aaccgctgta tcacaagaat atacgtgtga ccgacgccaa aacattggaa ctggtgaagc
    43561 aggctgcggg aacattgcaa ctggatatta ctgctcgcct gtcgatgagt ctcaataaca
    43621 cgccgctgca gggcgcgcat atcaacgtcg ttagtggcaa ttttattatt gcccagccgc
    43681 tgggcgtcga tgacggcgtg gattactgcc atagcgggcg tatccggcgg attgatgaag
    43741 acgcgatcca tcgtcaactg gacagcggtg caatagtgct aatggggccg gtcgctgttt
    43801 cagtcactgg cgagagcttt aatctgacct cggaagagat tgccactcaa ctggccatca
    43861 aactgaaagc tgaaaagatg attggttttt gctcttccca gggcgtcact aatgacgacg
    43921 gtgatattgt ctccgaactt ttccctaacg aagcgcaagc acgggtagaa gcccaggaag
    43981 agaaaggtga ttacaactcc ggtacggtgc gctttttgcg tggcgcagtg aaagcctgcc
    44041 gcagcggcgt gcgtcgctgt catttaatca gttatcagga agatggcgcg ctgttgcaag
    44101 agttgttctc acgcgacggt atcggtacgc agattgtgat ggaaagcgcc gagcagattc
    44161 gtcgcgcaac aatcaacgat attggcggta ttctggagtt gattcgcccg ctggagcagc
    44221 aaggtattct ggtgcgccgt tctcgcgagc agctggagat ggaaatcgac aaattcacca
    44281 ttattcagcg cgataacacg actattgcct gcgccgcgct ctatccgttc ccggaagaga
    44341 agattgggga aatggcctgt gtggcagttc acccggatta ccgcagttca tcacggggcg
    44401 aggttctgct ggaacgcatt gccgctcagg cgaagcagag cggcttaagc aaattatttg
    44461 tgctgaccac gcgcagtatt cactggttcc aggaacgtgg atttacccca gtggatattg
    44521 atttactgcc cgagagcaaa aagcagttgt acaactacca gcgtaaatcc aaagtgttga
    44581 tggcggattt ggggtaagcg atgaaaatcg ccggatgcga catgcgtaac acccgtgcgt
    44641 cgcatccggc aattacgttt attcccgcga actaaacaat gccgccagac cactgcgccg
    44701 ctcagtgcgc gtggcgattg ccgcactcaa aatacgctca tcggcataca gcgacaaacg
    44761 tcgacgcgcg cgggttaccg cagtgtaaac cagctctcgt gttactaccg gcgtgcgttg
    44821 gctcggtaaa atcaacgccg catggtcgaa ctccgatccc tgcgatttat gtaccgtcat
    44881 cgcccacgta gtttcgtgct ctggcaagcg actcggttgc acagacttaa tattgccgtc
    44941 cggcatcgca aaccagacgc gcgtcccctg cccacgatcc agagcgatac cgatatcgcc
    45001 attaaacaac ccaagcgcgc tgtcattacg ggcaatcatc accggtcggc cttcgtacca
    45061 acgagagtgc ggattacgat gaattttgcg cttctgttgc ataaactgct caattcgctc
    45121 attcagtcca gccacgccaa atggcccttc ccgcagggcg cacaaaagtt ggtactcatt
    45181 gaacgcctga atgattaaat ccggctcggc acgcgcttgc agcagatcca gataacgtcc
    45241 ataacccgca agagcttcct caagcatcgc aatataatct tcgccgctct gtaaaagccg
    45301 tttttcgata tcagaaaaat cctgctgaaa aacggttttc actgccgttt tatcaccacg
    45361 gttgatcgcc gcagctaact gaccaatgcc agaatcgctg ccgaaacgat agcttttttg
    45421 cagcaggcag agactgtcgc gcaaagatgc cgcttctgtg ccagttcctg ccggaacgtg
    45481 agttcccgtc aggcggctta gctgcccggc acgctcggcg gtaaagccgg cattggcata
    45541 agcacaaata tcgcccagca cagccccagc ctcaaccgag gccagttgat cacgatcgcc
    45601 gagaaagatc actcgcgcat gatcgggcaa ggcgtcgatc agtctcgaca tcataggcag
    45661 atcgatcatt gacgcttcat ctaccaccag cacatcaaga tgcagcgggt taccggcatg
    45721 atgacgtaaa cgctggctac ccggctgtgc acccagcaat cgatgcaaag tactggcatc
    45781 ttccggaatg cgtttctttt gttcatcggt cagcggtaac tgtcgcaaag ccttgccgag
    45841 agattcggtt aagcgcgcgg cagctttacc cgttggtgca gccaaacgga tacggcagcg
    45901 ttcgccgtcg gccatttgaa ttaacgctgc cagcaacttc gctacggtgg tcgttttacc
    45961 ggtgccaggg ccgccggaaa tcaccgagat ccgccgcgtc agcgccactg ccgccgcaac
    46021 tttttgccag ttaatttcat cgcttactgg aaaaagtttg tccagggttt gcgccagtag
    46081 agcttcatca acctcaatgg catgattcac ttcgttgaaa aagcgtgcca ctgtgcgctc
    46141 gttacaccac atgcgattca aataaagacg atcgccacag aggatcatcg gcgttggttc
    46201 atctccccgg ctgaccgctt gtgaagccag caagcattct tcccaatttt gtaactcacc
    46261 gatttcactg acacaggtcg ccaacagcgg atgcgacgcc tcgttatttt ccagtcgtga
    46321 gagcggtaaa cagacatgcc cttctccggc gtcgtggctt aacagggctg ccgcgagggt
    46381 gacggcagga tgttcatctc ccgccacggt cagggcaaat tgcacatcca gcgggcgtag
    46441 ctgtttgtgc tccacagctt ccagtaattg cttttgcaat ttcattacgc ctcctccagg
    46501 gtcataccgg caaacatctc atccatcagg tcaatcaacc cggcgttggg tcgggttgtg
    46561 taaatccctt gttgcggatg ttctttatca acgccacgca ggaacagata gataacgccg
    46621 ccaaagtgac gatcatagtc gtaatcagca atgcgatggc gcagataacg gtgcagcgcc
    46681 agggtataaa gctgatattg cagatcatag cggtgtgcct gcatcgccgc tgccatagcc
    46741 tgttgggtgt aagccgaact gtcttcaccc agccagttgg atttatagtc gagcaggtaa
    46801 taacgccctt cgtggcggaa taccaggtcg ataaagcctt ttaacatgcc gcgtacctgc
    46861 atgaactcca gcggcgggca gccagcggat agcggatcaa actggcgaat tagtgcatca
    46921 agctggctgg cgatgagcgg ttcactaatc ggcaggtaaa actccatctc cacctgttta
    46981 tcgcggtcgg aaagctgact caggctaaca cccgtttcat tgagaggtgc ctggaggaca
    47041 gccgtgatcc actcggtcaa caccggttcc cactgcgatt caaagccgcc gagttccagt
    47101 ttttcctgta cccagttcgg gtcaaccggc tgggtaaaat cgaggtcttc aaacaaactg
    47161 tgcaagaatg tccccggtga cgcaccgcgc gggaactgat gcggtgttaa cgtcggttct
    47221 tcaacgacgc tgaccacacc tgcggcatca acatccagcc gtggcatcag atcctgagcg
    47281 ataccgtgac cacgctgctg caaaccggag tagctggtga cacgccagtt atcgccgggc
    47341 aatcgttgta gtgtcctcgc attcagttct gcagttatag catcattaac ctgccagggc
    47401 tggttatcac ccgtttgtgc cgtttgccag gcaatatcat catcgcataa cgcttcaata
    47461 caggtgcgaa gccctgccgc atcttgcggt tccccttttt gcagcaaacg cccgagcgca
    47521 ctttggtgga cgtcggtgtc acctttttta tcgccacgac ggcgcaccag cggtgcaacg
    47581 ccgagactgc aatgccaaac cgaacgcgtc agcgccacgt aaagcaaacg cagatcttcc
    47641 gccagacgtt cggcctccgc gaggtcgacg ctttctggcg cagcattaag atccagaaca
    47701 gcctcaaacg agtggcgatc gtgatagaac gcctgatcct ggacgcggaa attggtaata
    47761 aatggcaacc agaccagcgg atattccagc ccttttgatt tatggatcgt gacaatctgc
    47821 accagatgtt tatcactttc gaggcgcatt tgttggctgg aggcattact gtctggctcg
    47881 aggatatgtt gcgataacca gcgtaccagc gcatgttcac tttccagctg cgttccggct
    47941 tcttgtagca gttcgctgat atgcaagata tcggtaagac gccgctcacc gcctgccgtt
    48001 gccagcaagt tttcagcaat gttacgcgcc gacatcagcg cccgcagcat cggcataacg
    48061 ccacgtttgc gccagatttg ccgataacca tcgaactctt cgactaccgc atcccacgca
    48121 tgttcgtcat tgttcagcgt ttcgatatcc agcgcgttca gccccatcat tgacgttgcc
    48181 agcgcactac gcagggtgtt ctcacgttcg ggcgtcatca ccgcctgcaa caaccaaagc
    48241 atttcctgcg cttccagagt ttcaaaaaca ctgtcgcggt tcgaaaggta aacggaaggg
    48301 atttccagca acgttaaggc gtcgcgcacc tgggcggcct cctggcggct gcgcaccagc
    48361 acactgatgt ccgaagcacg caccggacgc gcgtcgtcgc cgttcatcag caacgcttcg
    48421 ccccgctgtc cggcttgtag ccagtcgcgg atttgcgcag cacatacctg cgccatggta
    48481 ctttgatagt cgccaacgcc gcagctttcg ccttccatca gccacatttt catcgcaggc
    48541 tgcgtttcac ctttaaacac aaaacgtaac gcctgatttt tcccggctga tttcactgga
    48601 ataaaaggaa tttcgcgaaa catgaacgcg tcatcagtct gactgaaaag cttattcacg
    48661 ctgttcacca ttcctggtgc ggagcgccag ttggtatcta aagtgtagtg ggcgtgaact
    48721 tcgctacgcg ccttcatata agtgaagata tccgcacccc ggaatgcata tatggcctgt
    48781 ttcgggtcgc caatcagcaa caatgcggtt tccggctgat ggtgccagat acggcgaaaa
    48841 attcggtact gctgggggtc ggtatcctga aattcatcga tcattgccac cgggaatcgc
    48901 gtacggatcg ccgctgccaa cacctcgccg ctttcgctac gcagcgcgga atcgagccga
    48961 cttaacatgt catcaaaacc caattcgcca cggcggcgtt tttcacgcgc tactgtttcg
    49021 cggatctcag ccaatgcgcg ggttatcagc agatcgcgga tcgacaatgg ttctgcaagc
    49081 agttgatcga tcgcctcaaa cagtggatgt cgcggggttt cccccccggc tttcgtgcga
    49141 tcttctaaga aacgctgaga gaatttttcc agcgactccg gtaactgata actgttggtc
    49201 tcttcttctg cccaggcgct gatcttgtcg atccatttag cctgattgct acggttaaac
    49261 ttgcgtcgat caataccaga agattcgatc agcgcatcca gttcacccac cgcgtcgcgc
    49321 cactgctgtt ttacggcgtc gatacgcgcc acaatttgcg cgtggcggga agccagcgtt
    49381 tcatcatcgg gcggcggtgc tttgataacc ggtgcttcgc cttgcagata acgattaata
    49441 tcgcgcagca acgcctgcgg ccctttccag gtttcaaaga ccacctgggc aatttcacgc
    49501 ggcagcgggt agcagtggcg acgccagaaa tcggcgcagg cctggtagcg tagcagagac
    49561 tcatcttcaa tcagctgctg ctcaaacagc atgccggatt caaaggcatt caggttgagc
    49621 atgcgctggc aaaaaccgtg aatagtaaag actgccgctt catccatctg ccgttcggct
    49681 aacaacaacc actgcgcggc ttgcgcttta tcgtcgatct cttccagcag gcgttcgtac
    49741 agtggattgt cggtggtttc acgcagacag gcgatgcgca actcatggat attgctacgg
    49801 atacgaccgc gcaattctgc cgtggcagcc tcggtaaagg tgaccaccag cagttcttca
    49861 acggtcagcg ggcggggaaa ggcggcagaa ccgcctagtc caagtaacag gcgcaaatag
    49921 agcgccgcaa tcgtaaaggt tttgcctgtg ccggcagagg cttcaatcag gcgctcgccc
    49981 tgtaagggca agcgcaaagg atctagtgtc tcggcgacat cactcattct tttcactcat
    50041 caggggcatt gtttgctgca acgcgctgac gttctcccac actttccagc cttcaggatg
    50101 cacatattcg gctttcccgt tctggctgcc agaaatctgc gacagaatag ccatgccttg
    50161 cggctcgacc accgcctgat ggaagaaatc agcaagtttt tgcggcgtca gcagttttat
    50221 ctgggccacg attttatcac gcgaatcgaa gcgcatattg ccgcgatcga aatctttact
    50281 taacttcgat gcttcttcgc cgagcgtttg cggtgcctgc agcatctggg ttattaccgc
    50341 ctgctggatt tgcgcaaact cttctggctt catcgtccgc aattttgcct ctgcggttgg
    50401 gaaaaacgcc ttgtaacgct cccacaagaa tgaaggctgt ttatcattgc tttgcaacag
    50461 gaagcccatg ccccactgac gccccacgct cattggaaac gcaaacacgg catagcccaa
    50521 ctgttcttcg gtacgcaact gattgtagaa ccacggctgt acgatctgcc ccaacagaga
    50581 gctataggct gagctggtgt attcatcgta gccagtcggt acaaataccg ctgccagtgc
    50641 ggagtcggtg ctgttaccgg ctttttcaaa gatgacggat tgttttttat cgaccacgac
    50701 atctttgtta cgacaccact ccgaaccatc agcgcccaac tgtttttgca catcgcgtgc
    50761 cagcgttgtt gcctgggctt cggtcatgtt gccaataacc ataaactctg gtcgagcccc
    50821 tgattttaag gcgtcgcgat aggccagcac ctctttcaaa gtaatggagg gcaaaatttt
    50881 gcgccgttca tctcgcgaga agtacggcac ttgcgagagc atctgggcgg gcataatcgc
    50941 ctgctcgaac gctttgccct tttctgcaga atccatcatc tggttatacc aggacttcgc
    51001 ctgctcaagc tgatcttccg tagcggtata gctaaagtac ccctcgagca atgcctggaa
    51061 cagctgcggc aggcgctggg tgtaaccatt ggcattaacc ataaggccgt tgttagcatt
    51121 ggtggaaaaa cttatgccac caaccgacgc ctggttgctt aactgatcaa gcgccagccc
    51181 tgcgagataa tcattgagcg caaacatcac ctgattgcgg gcgctgtcca tggctttcgg
    51241 gttacgcaaa atcaggctga catcagcttt gggttcgctg gaaaaataac ggcttggcgc
    51301 atacaccaca cgcagattcg actcatcaac aatcagctct ggatggtcat atttcttctc
    51361 tgacttaatc agcgagaaat cgtcaggaat ataggggtta agctctggca aagagagcgc
    51421 aatgtcggcg gcttttttct gccagtcagc gaaagtttgt gcgctaattt tatcgacctg
    51481 atacggcgca tcgacaaagt aagccgtttt gttgtgcggc tctttcgggc tgatatacca
    51541 gatacgcgca ttctgcggcg tcatcatcgc caggcgttct tttactgctt tagcgtcgta
    51601 ccgatcggca atattgactg catccagcgt atgctcaaca ggaacgcgaa tcatggtatc
    51661 tgccagccat tcgacgtaat ccatatcacg ggtgattgac ggataacgga agtcgatatc
    51721 cagcacattc gccagttcat cgaagtattg tttatcaatc cctttttcgc gtaacagatt
    51781 gaggtagcta aaaatggccg ccacaacctg atcgcgattc gccagacctt tatcggttaa
    51841 agacgcagag atcgctaata cgccgctgtt gccgttgacg ataggatcgg aattggcgct
    51901 aatgccctca actaatccct gcttttgcag ccagtcagaa agtgtacctg ggctgcgatt
    51961 accgatcaga taggtaatca attcatccgt tttactacgg aacttcgctg aattgttatc
    52021 gatgcgaaac tcaacgcgca gaactttacg cggcagcgcc gggacgtaat gaatgataat
    52081 gcccttttgc gcgtcggtga ctaccggcac ggtgatttcc ggttttttgc tctctttgtt
    52141 cggcacgcga ccaaaggtgt ccgccgccat ttttgccaac tccggcagcg gtttattgct
    52201 gtaaataacc gccttcatca gattggcgga atagtacttc tcgtggaaat ctttcagcgc
    52261 ctgctgtacc ggatttccgg gtttgtcgct taaagtttcg aggttaccac cagaaaactt
    52321 tgaaccgggg tgtgccgggt taatggtttc tgcgctgacc tgtgccatgc gcatcccgtc
    52381 acgcgtacgc gccatggtta attcggcatt cactgcatta cgttcacgtt cggcgtattt
    52441 cttgtcgagc aagggttctg caatagcgtc cgccaggcgg tctaccgcac cgggcaaggc
    52501 gtcgttctca acttccagat agaaagccgt gcgatacggt gccgtgctgg cattgtgact
    52561 gccgccgtgc attttgagat attcggccag actgtcagcc tgcgggtact ttttcgaccc
    52621 catcagactc atatgttcaa ggtaatgtgc cagcccctgg tacgcctcgg gatcttccag
    52681 cgacccaacg ggcactacca gcgccgagag cgatttaacc gcctgcggat cagaaaccag
    52741 caagaccacc ataccgttat ccagacgtat agcctgatac tggcggttat ctttatcact
    52801 tttacggatg gtttcctgaa tcggctgcca tcccgtttct gcctgactta agggtgccca
    52861 aagggcaaca aacaacaata atgctttgaa ccaggtgctg cggggcattc acggacctca
    52921 taatcaactt tattttctgg ccagattcaa caggttaacg caaatcatct gccagaattt
    52981 aatcttgtgc tgcacgagtc agcctatgtc tatatatcca tcagtccgtg actggtgcgc
    53041 atcataaagc aagcagatat attgcgcaat ttttatacag cactcatgac tgattaaagc
    53101 gaaacagcgg taacaggaaa cgttgcgact gttcaacgat ggcctccatt gtctctggtg
    53161 ttaattgccg ccagagccgt tgataccaga tatcatcacc ttcgccacgc accatcatgt
    53221 tgccttcgta agcctgaagg aatttcgtac gggctttttg taacgtggaa tcgtcatcca
    53281 gcatggcatc gttttgcgcg tcataacagg tttttagcca cgcgccgcca ctttcaggta
    53341 acaccagcaa tggcgcggac attccttcac gatacccctc aatcagttgt gagaggtaat
    53401 gcaaagcctg ttcggctgca agcggcggaa aacgccactc gccgtctttg cgtagaaaaa
    53461 ggcgactttc accattacca ccgctggcac agtagacaag gtgttccagc caaagttgca
    53521 ttccctgcgc cacacttaat aaagagggac gccagcgcaa caggccatcc ggctgcacct
    53581 gcggcaacca gccagttatc tgcacaccgt tgcaggcgag atcaatttcc atactctgcc
    53641 ccggctggcg acaggcaata actctgtcgg caagctgctg catctcctgg cactgtgttt
    53701 cccagaaaat ttcaccaaaa gcgccatacg gtaaatcccc tgccgcccgg aagcggcgga
    53761 acaagcgttc ggcatcatcc tgctcaacca gtgcattcaa taactgctga ttgatttgat
    53821 aacggctaag cccttccaga ataaatggct cggtgtcggg gatttcgctg tcttcagtac
    53881 ggaagttcac ctgcaaacgc atctggaaga acgcccgtac cggatgtgcc cagaatcgtt
    53941 gtagcgtttc cagcggcacg gtttccggta aggtaaacgg cagcggctga acaaattcag
    54001 aatgtgcttt accagcctgg ctggccgcag gtagccattc acgagcatag ctttgtcgtt
    54061 cgcctggctg gtagttttgc ggatcaaacg gcatccgggt atggaggcaa gtaagatgcg
    54121 cttttaccct tgcctcgctt tcatcacagt tgagcgcttc atcgcccggt agataatggc
    54181 tttgcccgat gtagtcgatc agttcctgca ccagtaccga cgggaaacgt tcactgttat
    54241 cctgaataga acgcccgata tagctgatat agagtttttg ctgcgcggaa attaacgctt
    54301 ccaggaacag atagcggtcg tcatcgcgac gactacggtc gccacgcttc ggtttctggc
    54361 tcatcaggtc aaagcccaat ggcgcaagct ggcgtggata aacgccgtcg ttcattccca
    54421 gcaggcaaac cactttgaat gggatggaac gcattggcat cagagtacaa atgttaaccg
    54481 gcccggcgag aaaacgctgg ctgatacgtt cctgatccag gcgctgtgcc agttcatcac
    54541 gcaatagtga cagcggcacc gcgtcgccat actgcgcacc taaaccttcg gcgataatcg
    54601 cctgccattg ttgttcgatc agcgtcatcg ccgcttcggt ttccgcatcc ggcaggaaga
    54661 aggcgttgag catatcgcga caaaccggca accactcttc cagcggacgc tcctgcgcca
    54721 gcccgcgacg ccagatgttt agctgcatta gcagtgaagc cagatgcccc accagttctg
    54781 caattaagcc gctcgattca tcataaggta gaaccgattg ccactcgccc tgcgtactct
    54841 ccatcgcgta gcccagcaac atgcgcgtca ggccaaaccg ccaggtgtgt tgaccggtag
    54901 cggggagttc cagctcgcga acgttgtcgt catctatccc ccaacgaatg ccggattcgt
    54961 tgacccactg gcgtaaataa cgcagccctt cttcggtgat gtcaaaccgc gccgccagca
    55021 ccggcacatc cagtaatgcc agaacgtcct ccgacacaaa gcggctgtct ggcaacgata
    55081 acaggctgat aaacgcttca agtacaggat gcgactgccg cgcccgacgg tcggaaatgg
    55141 cgtaaggtag gtaacgatcc gcaggtgcgc taccaaacac tgcctgaata aacggactgt
    55201 agctgtcgat gtcggccacc atcacgatga tgtcgcgcgg agtcaatgtc gggtcttcct
    55261 ccagcattgc cagcaggcga tcgtgtaaaa cttcaacttc acgctgcggg ctatggcaaa
    55321 cgtggaaggt gatactgtta tccagtggat caagcaggcg tttgttatcg ctacgggaaa
    55381 actcttcgat attcacaccc gcaacagcgc agttttcaag ttccagaatg tcagactgaa
    55441 tgttatgcag caggttatct ggcgtgacat cgacaaaggc gtccagctcc tggctgccct
    55501 ccagatcaga aaggagataa atgtagtcgc gcccaagctt gccccatgag gccagcagcg
    55561 ggttgccgac atcctgttca ccatcgctgt taaagagctg cccggcattt tcgctgtcgc
    55621 gaaatagcgg taattcgcga tcttcaaaac tgtgtcgacg ctgacgagtc agcagtttcg
    55681 ccagataagc aggatcttta atgtcgcccc agtaataacg gcaggggttg gtaaacagga
    55741 gatggatttc aatatgttta cccagcgcct gtagcgcctg gagataaaca ggcggtaacg
    55801 cggaaatacc gcatataaag acgcgcgaag gtagccccgg ggggcaggtc gtcgcggact
    55861 ccagcgtttc gataaagcgc tgatagagat tggcgcggtg ccagcgcggt tgcccgagtt
    55921 gatgggtata ttccaccaac gccttccaca acggcgcttg ccaagcctgt gcttctccca
    55981 gcccttcaac caaatgtcct gtttcccact gtgccagcca gtccggacga tagaccagat
    56041 actggtcaaa caggtccgcc gcttttgaag aaagctggaa cagttttcgc ttgtcgctat
    56101 cgtcagtcag ataatgccgc aacagggtaa agtcttcgcg ctccaacagt tgcggcagca
    56161 gagtcatcag tttccagctc atgctctgtt tgttaaaggc actctctttg gggatctccg
    56221 gtaacacccg gacgaacata tcccatataa agctcgctgg cagcggaaaa tcaatatttg
    56281 ccgcaatacc aaacttttgc gacagggtca tttgcagcca ctgcgccata ccggtacttt
    56341 gcaccagaat catctctggt tcgaaaggat cgtccagccg ttcgcgttcg acaataaact
    56401 ccatcaacgc ttccagcacg tccagacgat tggaatggta gacccttaac atagcggctc
    56461 ctgactactg acgattcggg caatgcaggc gggtcatctc gccttctctg cccccgggtg
    56521 aaactagcgt aacgctgatg ctgacacatc ccgcttgcga tgtctgcatt cggttgacct
    56581 gccagtgggc aggtggcgaa atcgcacgca gttgcgtttg ctgccagcca tgccgccaga
    56641 gctgttggta ctggtttcta ctggcaagac tgttcattaa tgttcgctgg aaacccgata
    56701 acgcagtgac aatcatcacc atcagcacca ttgccaacat cacctccggg aggctaaagc
    56761 cttgttgatt cttcaaggaa gctgacataa cgccctctct ttcagcggac aaaaatcgct
    56821 ccagccgcgt ggcgaaaaaa caatgtttcc atcgatgact tcgcctgttc gccacaacga
    56881 aacgccttca taaccggcaa tcaataaggc ctcattatct gccaataaac gcagacaaac
    56941 ccgggcaccg gtggcagcgt acagtaagca ctgaactgcg ggctgcgtct gccaggagtg
    57001 catttttccc cactccagcg ccgactgaac aatggcctgg cggcgcaatg actgactttc
    57061 catgctcacg cgagaggcaa aactgcgatc ctgctgactc attccttgta atagcaagct
    57121 acccaaaacc agcagcatca ggaccagagc cagtgacgaa acaccctttt cgcggttcac
    57181 aggttgaatc ctgtcacgct atagctggca tccaccacgg tttgcggttc agacttactg
    57241 gcagcacgca tattaaccgt caacaccggc gagaagccgc tgacatcctg acgtacgacc
    57301 tgaaaagtgt cgataatgat ggcatccgga ttagtgactt tatcccagcc cttaccttca
    57361 caggatgtcg caccgcgtag cgtttccagc acatgctcct tcagacgaaa tccaatctgg
    57421 tcggactctt ttaccggttc gcgatcccag ataccgttac tgttcgcatc ccactgcaca
    57481 atgacacagt caccctgtcc gacaatttcc agcccttcgc cggtacagct gccatgacaa
    57541 taacccgccc tctggagatg cttcgcaacg gtaaataccc gcagccagat ttcatcttcc
    57601 agcgccagct tacgggtgct cgttaaactt tcacgctgta acgcaggcag aaagcgtgct
    57661 gcccccagca acaatacgct actgatcgcc atagcaatca acacttccag cagagaaaaa
    57721 cctagctctt ttacaggcat ccttctgttt ctccttgctg acaaagccgg agtcttcccc
    57781 acggcgaaac caccagccac cactcgcccg ttgagttttt gaagcgaata tgcccggccc
    57841 atgcggtatt gcgtaggcca aagaaagcaa gcgaaggtgt caggtcgctc atttcgactt
    57901 cgggccagcg tggcacaaag accaatggtg aactgccatg gcaggtattg gccccagcag
    57961 cggaactcac aaggcaccat aacgtcccct ccctgataac actgatactg tggtcgcggt
    58021 tatgccagtt ggcatcttca cgtaaataga gcaaatagtc ccgcgcctgg ctggcggttt
    58081 gccatagccg ttgcgactgc tgccagtatt gccagccata gagtccactt gcgcttagca
    58141 tgaccagaat cagcatcgcg accagcgttt caatcagcgt ataaccacgt tgtgttttca
    58201 tgccggcagt atggagcgag gagaaaaaaa gacgagggcc agtttctatt tcttcggcgc
    58261 atcttccgga ctatttacgc cgttgcagga ggttgcaaaa tttcgggaag gcgtctcgaa
    58321 gaatttaacg gagggtaaaa aaaaccgacg cacactggcg tcggctctgg caggatgttt
    58381 cgtaattaga tagccaccgg cgctttaatg cccggatgtg gatcgtatcc ttcaatctca
    58441 aagtcttcga aacggtagtc gaagatggat tcgggtttac gtttgataat caacttcggc
    58501 agcggacgcg gttcgcggct taattgcaga tgagtttgat ccatatgatt gctgtacagg
    58561 tgcgtgtcgc caccggtcca gacaaaatca cccacttcca gatcgcactg ctgcgccatc
    58621 atatgcacca gtaacgcgta gctggcaatg ttgaacggca ggccgaggaa gacgtcacag
    58681 gagcgctgat aaagctggca agagagtttg ccgtctgcca catagaactg gaagaatgca
    58741 tggcacggtg ccagcgccat tttatccagt tcgcctacgt tccacgctga aacaataatg
    58801 cggcgcgaat ccgggtcgtt tttcagctgg ttcagtaccg tagtgatctg gtcaatatga
    58861 cgaccatctg gcgttggcca ggcgcgccac tgtttaccat acactggccc gaggtcgccg
    58921 ttttcatcgg cccattcgtc ccagatggtg acattgtttt cgtgtagata agcaatgtta
    58981 gtgtcgccct gcagaaacca cagcagttca tggatgatgg aacgcaggtg gcaacgttta
    59041 gttgtcacca gcgggaatcc atcctgcagg ttaaaacgca tctgatgacc aaaaatggaa
    59101 agcgttccgg ttccggtacg gtcgtttttc tgtgtgcctt cgtcgagcac tttttgcatc
    59161 agttctaaat actgtttcat ggttcctcag gaaacgtgtt gctgtgggct gcgacgatat
    59221 gcccagacca tcatgatcac acccgcgaca atcatcggga tggaaagaat ttgccccatg
    59281 ctgatgtact gcacccaggc accggtaaac tgcgcgtcgg gctggcggaa aaactcaaca
    59341 atgatgcgaa acgcgccgta gccaatcagg aacaagcctg agactgctcc cattgggcgc
    59401 ggtttacgaa tatacaggtt gaggataata aacagcacca caccttccag cagcagctcg
    59461 taaagctgtg atgggtggcg cggcagcaca ccgtaagtgt cgaaaatgga ttgccactgc
    59521 gggttggttt gcagcagcaa aatatcttct gtacgggagc cagggaacag catggcaaac
    59581 gggaagttcg ggtcaacgcg gccccacaat tcaccgttaa taaaattgcc cagacgcccg
    59641 gcgccaagac caaacggaat gagtggtgcg ataaaatcag agacctggaa gaaggaacgt
    59701 ttagtacggc gggcgaagat aatcatcacc acgataacgc caatcaggcc accgtggaaa
    59761 gacatgccgc cgtcccagac acggaacaga tacagcggat cggccataaa ctgcgggaaa
    59821 ttgtagaaca gaacataacc aatacgtcca ccgaggaaga cgccgaggaa gcccgcatag
    59881 agtaagtttt caacttcatt tttggtccag ccgctgcccg gacgattcgc ccgacgtgtt
    59941 gccagccaca ttgcaaaaat gaaacccacc agatacatca ggccgtacca gtgaagcgct
    60001 acgggtccta ttgagaaaat gaccggatca aactccggaa aatgcagata gctactggtc
    60061 atctgtcacc acaagttctt gttatttcgc tgaaagagaa cagcgattga aatgcgcgcc
    60121 gcaggtttca ggcgctccaa aggtgcgaat aatagcacaa ggggacctgg ctggttgccg
    60181 gataccgtta aaagatatgt atatgatccg cgctataacc ctccgcgaat caacccgccc
    60241 atgccgcgac gctccataaa ggctgcaacc tgatggcgaa cttcggtcgc cagttgcgct
    60301 tccagactac gctgcgcaag attttctgct tcggcataat caatgcgccg cagtaggtat
    60361 tttacgcgcg ctacagaacg tccgttcata gacagatggc gatacccaag cccaatgagg
    60421 attgccacgc acatgggatc gcccgccatt tcaccgcaca aacggagatc gataccatgt
    60481 atttccgctt cccgggcgat catcgccaga gctcgtaaca ttgcaggatg aagactgtca
    60541 taaatgttcg ccacccgggt attgttgcga tcaacggcca ggatgtattg agtcagatcg
    60601 ttggtgccaa cagagatgaa atcgacccgc tttgccagat gcggcagcat aaataccatt
    60661 gacggcactt ccagcatgat gccgatacgt ggtttgggaa tttcgtaacc gatcatctcc
    60721 tcgacttcac gtccggcacg ttcaatcagg cggcgtgctt cgtcaacttc atcgaggctg
    60781 gtgaccatcg gcaacagaat attcaggttg cccgtagcgg cattagcacg cagcatcgcc
    60841 cgcacctgga tcaagaagat ctccggctga tcgagcgtaa tgcgaatccc acgccaaccc
    60901 aggcatggat tctcttcgct gatcggcatg taaggcagct gcttatctgc tccgacatcc
    60961 agcgtacgca aggtgacggg tttatcattg aacatttgca gcatcccctg atactgcgcc
    61021 acctgttctt cttccgacgg aaaaccactt tgcagcatga atgggatttc agtgcgataa
    61081 agtcctatgc catcaatacg gctgcccagt ttttcttcat gttccgggct taaaccggca
    61141 ttgagcatga cttttatacg ctcgccactt tttaactgcg cgggtaaatt gacgtcatct
    61201 tccgccagac ggctaagctc aatctcttca ctaattagcc gctgatattc ttgcagcagt
    61261 accggctccg gatcgaccag caattcaccg cgataaccat cgacaatcag cgtccgacga
    61321 tgcagcaccg aaggctgaat atccgcgccc atcacggtag ggatccccag cgcacgtacc
    61381 atgatcgcag catgggagtt ggctgcgcca tcgcgcacga caacaccgac taagcgatcc
    61441 tggggcagct cagcaagcgt tgtcgctgac agttcatctg ccaccagaat gaaacgttcc
    61501 ggccaggcgt tcggcccttg attagcgtca tcaagatgaa acagcaatcg ctgccccagc
    61561 gcacgtaaat cgccagcccg ctctttgaga tagttatcgc ttagcgcggc aaactgttcg
    61621 gcaaattttt caatgaccgt ttttaccgcc cactctgcca ccgagccttt atcaacctcg
    61681 gcaaacaatt cgcgccgcag ccgggtgtcc gaaagcaggt gcgagtaaag atcgaaaata
    61741 gccgccgttt ctttttgtgc accggcggca aagcgtttgc tgtagcggcg aaactcgttt
    61801 gccgcttctt ccagcgcccc ggtcagtcgt tcgcgttcca gagccggatc cagcgttgat
    61861 gcctgataca cctgttccat taaaggtaac gtggcatcct gccagccttc ggcaatcgcc
    61921 acaccaggcg ctgccggtaa tgcacggatt cgcgtctggc gatattgtcc aaacaaggca
    61981 gtcaactgcg actgagaaag aatagctgcc atctgagtgg caagcgtcac caggaaggat
    62041 tcttcacttt cgtcatactg gcgcaactct cgttgctgta ccaccagtac accaagcaac
    62101 tggcgacgtt gaataattgg tacgcctaaa aacgcgcgga aacgttcttc ttttacggag
    62161 gggatgtatt tgaagctggg gtgcttttgc gcatctgcaa ggtttatcgg ttccgccagc
    62221 ctgccaacca ggccgacgat cccttcatca aacgcgagcg ttacagtgcg accgcgtggt
    62281 tttttcagcc cccgggtcgc catcaggtag taacaacgtc gatcatgatc ggcaaggtaa
    62341 accgaacaga cctcggtatc catcgcaaga cagatgtcgg taaccagaat atttaacgcc
    62401 tcattcaggc gtggtgcgct ggctaccttt tcgactattt cgcgcaggcg agtgagcata
    62461 attggcgtga cttaacctct tttacgtcga taagcagatg cgttttgtgg ttttggcgta
    62521 ttttcctgca gtgacatcac cacactcgcg aactctttca ttaccctacg gtagacatca
    62581 cgtttaaatg acaccacctg tctgaccgga taccagtaac ttacccatcg ccagccgtca
    62641 aactctggtg tactgctggt ttgcatattg atttctgcat cgccgctcac cagctgcaag
    62701 agaaaccatt tttgtttttg gccgatacaa accggcttcg tgtcccaacg caccaaacgt
    62761 ttcggtaatt tgtagcgcaa ccagttacgc gttgaagcaa ggattcgaac gtctttacgg
    62821 cttaatccta cttcttcaaa caattcacgg tacatcgcct gctctgcgga ttctccgggg
    62881 ttgattccgc cttgcggaaa ttgccaggag tgctgaccaa atcgccgggc ccacattacc
    62941 tgcccctggc gattacaaat cacgatacct acgtttgggc ggtagccatc gtcatcaatc
    63001 accggactac ctcaaaataa agctttatat acgaatgatt gtttcatact ccaggaagac
    63061 ggtaaaccac tctctgcagg gcattacaca ctaataacaa ttgaataact cacagttatg
    63121 tgcagagtta taaacagagg aaggggtgga tagccgtttt tattcacctt ttctgtg
//
LOCUS       DACBVI010000010        60528 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.010, whole genome shotgun sequence.
ACCESSION   DACBVI010000010 DACBVI010000000
VERSION     DACBVI010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.010"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(277..1308)
                     /gene="rsmC"
                     /locus_tag="IU325_03190"
     CDS             complement(277..1308)
                     /gene="rsmC"
                     /locus_tag="IU325_03190"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HAP5808044.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1411..1824
                     /gene="holD"
                     /locus_tag="IU325_03195"
     CDS             1411..1824
                     /gene="holD"
                     /locus_tag="IU325_03195"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HAP5808045.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1793..2239
                     /gene="rimI"
                     /locus_tag="IU325_03200"
     CDS             1793..2239
                     /gene="rimI"
                     /locus_tag="IU325_03200"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP5808046.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2254..2931
                     /locus_tag="IU325_03205"
     CDS             2254..2931
                     /locus_tag="IU325_03205"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HAP5808047.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3022..4611
                     /gene="prfC"
                     /locus_tag="IU325_03210"
     CDS             3022..4611
                     /gene="prfC"
                     /locus_tag="IU325_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP5808048.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            5004..5609
                     /gene="osmY"
                     /locus_tag="IU325_03215"
     CDS             5004..5609
                     /gene="osmY"
                     /locus_tag="IU325_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HAP5808049.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5736..5897
                     /locus_tag="IU325_03220"
     CDS             5736..5897
                     /locus_tag="IU325_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HAP5808050.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6019..7092
                     /locus_tag="IU325_03225"
     CDS             6019..7092
                     /locus_tag="IU325_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HAP5808051.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYCDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            7089..7868
                     /locus_tag="IU325_03230"
     CDS             7089..7868
                     /locus_tag="IU325_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP5808052.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     KPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYTLFNVP"
     gene            complement(7902..8765)
                     /gene="yjjW"
                     /locus_tag="IU325_03235"
     CDS             complement(7902..8765)
                     /gene="yjjW"
                     /locus_tag="IU325_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HAP5808053.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLCHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKVLGDVPVRLNAFHAHGVYGEAQSWPSATPEDVEQLADALKVRG
                     VSRLIFPALYL"
     gene            complement(8737..10287)
                     /locus_tag="IU325_03240"
     CDS             complement(8737..10287)
                     /locus_tag="IU325_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HAP5808054.1"
                     /translation="MPTSHEIALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLQEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFARTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAVNEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            10545..11324
                     /gene="deoC"
                     /locus_tag="IU325_03245"
     CDS             10545..11324
                     /gene="deoC"
                     /locus_tag="IU325_03245"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP5808055.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIDIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKDACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            11402..12724
                     /gene="deoA"
                     /locus_tag="IU325_03250"
     CDS             11402..12724
                     /gene="deoA"
                     /locus_tag="IU325_03250"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HAP5808056.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLTSGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            12776..13999
                     /gene="deoB"
                     /locus_tag="IU325_03255"
     CDS             12776..13999
                     /gene="deoB"
                     /locus_tag="IU325_03255"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP5808057.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            14056..14775
                     /gene="deoD"
                     /locus_tag="IU325_03260"
     CDS             14056..14775
                     /gene="deoD"
                     /locus_tag="IU325_03260"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP5808058.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(14936..15199)
                     /locus_tag="IU325_03265"
     CDS             complement(14936..15199)
                     /locus_tag="IU325_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP5808059.1"
                     /translation="MIDLENQEREIINLMFSQGISWITAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAGIYGCPPELLICPSWMTAEHK"
     gene            complement(15231..16247)
                     /gene="lplA"
                     /locus_tag="IU325_03270"
     CDS             complement(15231..16247)
                     /gene="lplA"
                     /locus_tag="IU325_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HAP5808060.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPEITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMA
                     GAVR"
     gene            complement(16275..16919)
                     /locus_tag="IU325_03275"
     CDS             complement(16275..16919)
                     /locus_tag="IU325_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HAP5808061.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRNDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            17025..17993
                     /locus_tag="IU325_03280"
     CDS             17025..17993
                     /locus_tag="IU325_03280"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HAP5808062.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            18042..19424
                     /gene="radA"
                     /locus_tag="IU325_03285"
     CDS             18042..19424
                     /gene="radA"
                     /locus_tag="IU325_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP5808063.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            19445..20677
                     /locus_tag="IU325_03290"
     CDS             19445..20677
                     /locus_tag="IU325_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide
                     kinase/transcriptional regulator NadR"
                     /protein_id="HAP5808064.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(20903..21088)
                     /locus_tag="IU325_03295"
     CDS             complement(20903..21088)
                     /locus_tag="IU325_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808065.1"
                     /translation="MPLINRIVMPPMTRSRAGDVATDIMADYYAQRASAGLIISEGTQ
                     ISRSAAHNFPRHADLLR"
     gene            complement(21137..21349)
                     /locus_tag="IU325_03300"
     CDS             complement(21137..21349)
                     /locus_tag="IU325_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAP5808066.1"
                     /translation="MKTIIAIFTASLFSVAAFNASAQSVTATGTTLDSAEAVIAQKAK
                     EMNASEYKITSARMGNTVTMSAELYK"
     gene            complement(21507..21923)
                     /locus_tag="IU325_03305"
     CDS             complement(21507..21923)
                     /locus_tag="IU325_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001699669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HAP5808067.1"
                     /translation="MAKTTVSIEIPASADAVWQLMGGFDALPDWLPFIPKSVVTEGGR
                     VRSLTTSDGGTVVERLEAFDNRQRSYTYSIIQAPFPVVDYLSTITVHETADSQVSRVE
                     WFGEFTPVNVTNEEAEALFTGIYRDGLKALKDNFVA"
     gene            complement(21949..22746)
                     /locus_tag="IU325_03310"
     CDS             complement(21949..22746)
                     /locus_tag="IU325_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP5808068.1"
                     /translation="MKLDLTGKVAVVSGSTSGIGQGIAAGLAKAGATVVVVGRKQRGV
                     DDAMAAIATQTPTASLRGVVADLSTEDGVAALIAAVPTADVLVNNLGIFNDKDFFSVP
                     DDEWMHFYNVNVLSGVRLARHYAPGMTEQGWGRIIFVSSESGVAIPGDMINYGVTKAA
                     NLAVSHGLAKRLAGTGVTVNAILPGPTFTDGLKEMLADAAAQSGRSAREQADEFVKTS
                     RPSSIIQRAADVEEVANLAVYLASPLSSATTGAALRVDGGVVDTLTM"
     gene            complement(22766..23776)
                     /locus_tag="IU325_03315"
     CDS             complement(22766..23776)
                     /locus_tag="IU325_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015368058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP5808069.1"
                     /translation="MSIKNLLPGKIGLGGAPLGNMFRAIPEEEAHATVSEAWNLGVRY
                     FDTAPLYGSGLSEIRMGEALSQYPRDEYVLSSKVGRIMLDEMEDPAKRDFGEKGGLFE
                     HGLKNKILNDYSADATLRSIEDSLKRLKTDRLDIVWIHDPAQDFYGDSWLEQFNIART
                     GAFRALTRLRDEGVIKAWGLGVNRVEPCELTLALDEPKPDAFLLAGRYSLLDHERALQ
                     RLMPEALEQNVDIVVGGPYSSGVLAGGEHFEYQKASAAIQQKVAQINAIATRFNVNVK
                     AAALQFALANPAVAAVIPGSSRPGRMAEDLAALNAPVPAEFWAEMRRQNLVAENAPLP
                     TR"
     gene            23910..24797
                     /locus_tag="IU325_03320"
     CDS             23910..24797
                     /locus_tag="IU325_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP5808070.1"
                     /translation="MIDLRQLKYFVVVAEEEHVGRAAERLHISQSPLSRQIAQLEERL
                     GLTLFERNQQRIRLTADGQTFLAETRSLLTHANRLESLGKRLGRGEDGGLCIGYIENA
                     MHSGVLSTALRTLRVSRPNVHIALYSQPPATQIEGLRQRSLDFSLVDTPPTDSEQDLE
                     YIQVLDDRMLLALPQNHPLAQEENLVASQLGDEQWIAVIHNDDAHSRDHFVAACVNAG
                     FNPDIRLEAGEPLTALGLVAAGLGLALIQHSLRHNAPEGVVLRELPWMNYSTQLWAAW
                     HKANQRPLVSHFRQILRGE"
     gene            complement(24952..26619)
                     /gene="ettA"
                     /locus_tag="IU325_03325"
     CDS             complement(24952..26619)
                     /gene="ettA"
                     /locus_tag="IU325_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HAP5808071.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVNNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            26830..28767
                     /gene="sltY"
                     /locus_tag="IU325_03330"
     CDS             26830..28767
                     /gene="sltY"
                     /locus_tag="IU325_03330"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HAP5808072.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            28857..29183
                     /gene="trpR"
                     /locus_tag="IU325_03335"
     CDS             28857..29183
                     /gene="trpR"
                     /locus_tag="IU325_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HAP5808073.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKDH"
     gene            complement(29177..29692)
                     /gene="yjjX"
                     /locus_tag="IU325_03340"
     CDS             complement(29177..29692)
                     /gene="yjjX"
                     /locus_tag="IU325_03340"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP phosphatase"
                     /protein_id="HAP5808074.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLHPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQ"
     gene            29744..30391
                     /gene="gpmB"
                     /locus_tag="IU325_03345"
     CDS             29744..30391
                     /gene="gpmB"
                     /locus_tag="IU325_03345"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HAP5808075.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            complement(30388..31257)
                     /gene="robA"
                     /locus_tag="IU325_03350"
     CDS             complement(30388..31257)
                     /gene="robA"
                     /locus_tag="IU325_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HAP5808076.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            31468..31941
                     /gene="creA"
                     /locus_tag="IU325_03355"
     CDS             31468..31941
                     /gene="creA"
                     /locus_tag="IU325_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HAP5808077.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            31954..32643
                     /gene="creB"
                     /locus_tag="IU325_03360"
     CDS             31954..32643
                     /gene="creB"
                     /locus_tag="IU325_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HAP5808078.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            32643..34067
                     /gene="creC"
                     /locus_tag="IU325_03365"
     CDS             32643..34067
                     /gene="creC"
                     /locus_tag="IU325_03365"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HAP5808079.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKKITLHVTPTEVNVAAEPALLEQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            34125..35477
                     /gene="creD"
                     /locus_tag="IU325_03370"
     CDS             34125..35477
                     /gene="creD"
                     /locus_tag="IU325_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HAP5808080.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(35537..36253)
                     /gene="arcA"
                     /locus_tag="IU325_03375"
     CDS             complement(35537..36253)
                     /gene="arcA"
                     /locus_tag="IU325_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HAP5808081.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            36349..36489
                     /locus_tag="IU325_03380"
     CDS             36349..36489
                     /locus_tag="IU325_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808082.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            36889..37575
                     /locus_tag="IU325_03385"
     CDS             36889..37575
                     /locus_tag="IU325_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP5808083.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADKHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            37789..37854
                     /gene="thrL"
                     /locus_tag="IU325_03390"
     CDS             37789..37854
                     /gene="thrL"
                     /locus_tag="IU325_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HAP5808084.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     misc_feature    37790..37909
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            37935..40397
                     /gene="thrA"
                     /locus_tag="IU325_03395"
     CDS             37935..40397
                     /gene="thrA"
                     /locus_tag="IU325_03395"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HAP5808085.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            40399..41331
                     /gene="thrB"
                     /locus_tag="IU325_03400"
     CDS             40399..41331
                     /gene="thrB"
                     /locus_tag="IU325_03400"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HAP5808086.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            41332..42618
                     /gene="thrC"
                     /locus_tag="IU325_03405"
     CDS             41332..42618
                     /gene="thrC"
                     /locus_tag="IU325_03405"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HAP5808087.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            42832..43128
                     /locus_tag="IU325_03410"
     CDS             42832..43128
                     /locus_tag="IU325_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAP5808088.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPYGPPPSPRHNKHNDHRGDHRPGPDKHHR"
     gene            complement(43168..43944)
                     /gene="yaaA"
                     /locus_tag="IU325_03415"
     CDS             complement(43168..43944)
                     /gene="yaaA"
                     /locus_tag="IU325_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HAP5808089.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(44014..45444)
                     /locus_tag="IU325_03420"
     CDS             complement(44014..45444)
                     /locus_tag="IU325_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid carrier protein"
                     /protein_id="HAP5808090.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMALIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDTWDDVSQE"
     gene            45723..46676
                     /gene="tal"
                     /locus_tag="IU325_03425"
     CDS             45723..46676
                     /gene="tal"
                     /locus_tag="IU325_03425"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAP5808091.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            46791..47378
                     /locus_tag="IU325_03430"
     CDS             46791..47378
                     /locus_tag="IU325_03430"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HAP5808092.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARREVSE"
     gene            complement(47413..47979)
                     /gene="satP"
                     /locus_tag="IU325_03435"
     CDS             complement(47413..47979)
                     /gene="satP"
                     /locus_tag="IU325_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HAP5808093.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(48128..48841)
                     /gene="msyB"
                     /locus_tag="IU325_03440"
     CDS             complement(48128..48841)
                     /gene="msyB"
                     /locus_tag="IU325_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HAP5808094.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(48867..49271)
                     /locus_tag="IU325_03445"
     CDS             complement(48867..49271)
                     /locus_tag="IU325_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HAP5808095.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKVARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            49647..51563
                     /gene="dnaK"
                     /locus_tag="IU325_03450"
     CDS             49647..51563
                     /gene="dnaK"
                     /locus_tag="IU325_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP5808096.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            51652..52782
                     /gene="dnaJ"
                     /locus_tag="IU325_03455"
     CDS             51652..52782
                     /gene="dnaJ"
                     /locus_tag="IU325_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP5808097.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(52886..53095)
                     /gene="mokC"
                     /locus_tag="IU325_03460"
     CDS             complement(52886..53095)
                     /gene="mokC"
                     /locus_tag="IU325_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HAP5808098.1"
                     /translation="MLNTCRVPLTDRKVKEKRAMKQHKAMIVALIVICITAVVAALVT
                     RKDLCEVHIRTGQTEVAVFTAYESE"
     gene            53624..54790
                     /gene="nhaA"
                     /locus_tag="IU325_03465"
     CDS             53624..54790
                     /gene="nhaA"
                     /locus_tag="IU325_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HAP5808099.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            54856..55755
                     /gene="nhaR"
                     /locus_tag="IU325_03470"
     CDS             54856..55755
                     /gene="nhaR"
                     /locus_tag="IU325_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HAP5808100.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(55793..56752)
                     /locus_tag="IU325_03475"
     CDS             complement(55793..56752)
                     /locus_tag="IU325_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HAP5808101.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCAVTNVGEQRGTYILKPLSMKGNL
                     TTEKIENTGNIIECADLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGKLSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNFNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQDSGTSILKNDLENSEDGAGQIGFQLSNDGTHEMQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(56765..59215)
                     /locus_tag="IU325_03480"
     CDS             complement(56765..59215)
                     /locus_tag="IU325_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAP5808102.1"
                     /translation="MLRTTRWVAAIIFLYSFPGYAEETFDTHFMIGGMRGEKVSEYRF
                     DNKQPLPGNYELDFYVNHQWRGKKDITIPESPAKPCLPKVLLTTLGVKTDNLNTEDNC
                     ILLDEAVHGGQYQWDISEHRLNLTVPQAYINELERGYVPPESWDRGIDAFYTSYNLSQ
                     YRSYDSNNNSNTASYGRFNSGLNLFSWQLHSDASYSKPDDMKGKWQSNTLYLERGWSQ
                     ILSTVQIGENYTSSLIFDSLRFSGIRLFRDMQMLPDSMQSFTPLVQGVAQSNALITVS
                     QNGYTIYQKEVPPGPFTIADLQLSGSGSDLDVSIKEADGSVRSFLVPYSSVPNMLQPG
                     VSNFDFTAGRSQIYGVKNQEDFLEANYIYGLNNLLTLYGGTILSDNYNAITLGNGWNT
                     PLGAISFDATRSSSKLNNDTRHEGTSYQVAYNKYLLQTATHFSVAAWRYASQDYRTFS
                     DHLYENDKINHQSDYDDFYDIGRKNSLSANIMQPLSNNLGNVSLSALWRNYWGRSGNA
                     KDYQFSYSNSWQRISYTFSASQSYDENDKEEERFNLFISIPFYWGDDIAKTRHQINLS
                     NSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVNQQQQNNDTSLGTNLSWRTPIATID
                     GSYSHSKNAWQSGGSISSGLVVWPGGINITNQLSDTFAILDAPGLEGAHINGQKYNRT
                     NSKGQVVYDLIIPHRENHLVLDIANSESETELQGNRQIIAPYRGAVSYVQFTTDQRKP
                     WYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGSRLFIRVDEIPTGIKVALNDEQNLF
                     CTITFQHVIDENKTYICQ"
     gene            complement(59228..59911)
                     /locus_tag="IU325_03485"
     CDS             complement(59228..59911)
                     /locus_tag="IU325_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAP5808103.1"
                     /translation="MRIITALLMSFFILPVHAGVVIYGTRIIYPEKNREVLVQLMNQS
                     KNASLIQAWIDDGNTTIAPEKIQVPFILTPPVSRVAGGSGQQLKIRKMPNNLPHNKES
                     LFYLNVLDIPPNNPQNAGKNKIKLALQNRIKLLWRPSGIAPVDKKSLSQLNIKKKNNA
                     ISINNETANWITVTTIKAQNVKVNNESILLPPFSNNDITLKNNHASEYELTVVDDYGN
                     NIHSKIAAR"
     gene            complement(59961..60494)
                     /locus_tag="IU325_03490"
     CDS             complement(59961..60494)
                     /locus_tag="IU325_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP5808104.1"
                     /translation="MKKYIIPFVATTLMFSATTFAKDVDQGTLTIRGEVVDTTCYFVN
                     GDSTTDIVVEDVVTSAMNQLINNEIYTLKTGSTSHDLEIQCDGNTAPSIEVLASEFDA
                     NNFTLNKGTAENIGFAIFLDDGTLTRMRPGLKTPLKDNGSGQYFLNLSAQYARTSGNP
                     VTKGSVESTVTIKISAD"
BASE COUNT        14717 a        15044 c        16011 g        14756 t
ORIGIN      
        1 caccttcgca taccatcaat tcttaaaaag aattgctacc acggaggcgc attctagtgg
       61 ttttcagctt ttcgtcaata gttaattatc gccggaggtg taattgctgg aaaaatgtcc
      121 atcaggaaac tagcgtgcag gtttggtagg catgcggggg cagatgccag atgcgacgct
      181 ggcgcgtctt atctggccta cgaagggcta acgtgcaggt tttgtaggtc ggataaggcg
      241 ttcacgccgc atccgacacg gtattcggcg agataattaa cctttcttcg cctggcgggt
      301 cataatggcg cgatacacct tgaaacgccc ggtttgcgca atcacttcat ggaagccaaa
      361 tgtctcatcc agcacgtccg ggtaaggcag gaaggcgttc gctacaattc gcagctcgcc
      421 gccgctatta agatgacgca ccgcgccgcg aatcagcgtt tgcgccgcat ccaggctggt
      481 ttgcatccca tcgtggaacg gcgggttgga gatgatcata tcaaaacgac ctttcacctc
      541 ggaaaagacg ttgctggcaa agacttcacc ttcaacacag ttggccgcaa gtgttgcgcg
      601 gctggcttct accgctggcg cagagacatc gcacaaggtg agacgaattt tcggcgaatg
      661 gcgcgcaaag gcaactgaaa gcacccccgc gccacagccg acatccagca ctttaccttt
      721 cgtgtgcggg gttaacgtcg agagtaacaa ctggctaccg acatccagac cgtcgcggct
      781 aaacacgcca ggcagcgttt tgaccgtcag gccatcgacg ctgtattcgc cccagaattt
      841 atccgcatca aatactggct gtttttccag acgaccaaaa tagaggccac agcgacgagc
      901 gctgtcgact ttattcaacg gcgcataatc tgccagcatc tgctcggcgc tgcgcacgcc
      961 gctgcggttc tcgccaacga caaaaatatc tgtccccact ggcagcagag aaagtaaatt
     1021 catcaactgg aactgggctt ccggtttgtt cttcggccag tagtaaatca gcgtatcgca
     1081 atctgcgacg tcatccaccg tggcgaccag actgaaacgg gcgttatccc ccatctggcg
     1141 gcttaatacc tgccagtggt ggaattgctg ggtatgagca cggctggccg cggtatctaa
     1201 acgcgcgggc aggtcatcct gtaagtctcc ggcaaacaga atacggcttt gttcgaaatc
     1261 atcactgtga cgcagcaaga cttcacttgc cggggtaaat gcagacatgg aatgctcctc
     1321 aattgatact ggcggcgatt atagccatat gttggcgcgg tatcgacgaa tttgctatat
     1381 ttgcgcccct gacaacagga gcgattcgct atgacatccc gacgagactg gcagttacag
     1441 caactgggca ttacccagtg gtcgctgcgt cgccctggcg cgttgcaggg ggagattgcc
     1501 attgcgatcc cggcacacgt ccgtctggtg atggtggcaa acgatcttcc cgccctgacc
     1561 gatcctttag tgagcgatgt tctgcgcgca ttaaccgtca gccccgatca agtgctgcaa
     1621 ctgacgccag aaaaaatcgc gatgctgccg caaggcagtc gctgcaacag ctggcggttg
     1681 ggtactgacg aaccgctatc actggaaggc gctcaggtgg catcaccggc gctcaccgaa
     1741 ttacgggcaa acccaacggc acgcgccgcg ttatggcaac agatttgcac atatgaacac
     1801 gatttcttcc ctcgaaacga ctgatttacc agcggcttac cacattgaac aacgcgccca
     1861 cgcctttccg tggagtgaaa aaacctttgc cagcaaccag ggcgagcgtt atctcaactt
     1921 tcagttaacg caaaacggca aaatggcggc gtttgcgatt acgcaagtgg tgctggatga
     1981 agctacattg ttcaatattg cggtcgatcc tgactatcag cgtcagggat tgggaagggt
     2041 gctgctggaa catctgatcg acgaactgga aaaacgcggc gtggcgacac tatggctgga
     2101 agtccgtgcc tcaaatgctg ccgccattgc cctgtacgaa agtttaggct ttaacgaggc
     2161 gacgattcgc cgcaattact accccaccac ggacggtcgc gaagacgcca tcatcatggc
     2221 gttgccaatc agtatgtaag acaaggtgga ataatgaagt gggactggat tttctttgat
     2281 gccgatgaaa cgctgtttac ctttgactca ttcaccggcc tgcagcggat gtttcttgat
     2341 tacagcgtca cctttaccgc tgaagatttt caggactatc aggccgttaa caaaccactg
     2401 tgggtggatt atcaaaacgg cgcgatcact tcattacagc ttcagcacgg gcgtttcgag
     2461 agctgggccg aacggctgaa agttgaagca ggcttgctta acgatgcctt tattaatgcg
     2521 atggcggaaa tctgcacgcc gctgccgggc gcggtttctc tgcttaacgc cattcgtggc
     2581 aacgccaaaa tcggcatcat caccaacggc tttagcgcct tgcagcaagt gcgtctggaa
     2641 cgcacgggcc tgcgtgatta cttcgatttg ctggtgattt ccgaagaagt tggcgttgcc
     2701 aaaccgaata agaaaatttt cgattatgcg ctggaacagg cgggcaatcc tgaccgttca
     2761 cgcgtgctga tggttggcga cactgctgag tccgatattc tcggtggcat caacgccggg
     2821 cttgcgacct gctggctgaa tgcgcacaat cgcgagcaac cagaaggcat cgcgcccacc
     2881 tggaccgttt catcgttgca cgaactggag cagctcctgt gtaaacactg attgcctccc
     2941 ccccgttgat gggtaaaata gccgcaattt ttcgttttca acaagcgcgg cgcgatgccg
     3001 cttactcaag aagaaagaat tatgacgttg tctccttatt tgcaagaggt ggcgaagcgc
     3061 cgcacttttg ccattatttc tcacccggac gccggtaaaa ctaccatcac cgagaaggtg
     3121 ctgctgttcg gacaggccat tcagaccgct ggtacagtaa aaggccgtgg ttccaaccag
     3181 cacgctaagt cggactggat ggagatggaa aagcagcgtg ggatctccat tactacgtct
     3241 gtgatgcagt ttccgtatca cgattgcctg gttaacctgc tcgacacccc ggggcacgaa
     3301 gacttctcgg aagataccta tcgtaccctg acggcggtgg actgctgcct gatggttatc
     3361 gacgccgcaa aaggtgttga agatcgtacc cgcaagctga tggaagttac ccgtctgcgc
     3421 gatacgccga tcctcacctt tatgaacaaa cttgaccgtg atatccgcga cccgatggaa
     3481 ctgctcgatg aagttgagaa cgagctgaaa atcggctgcg caccgatcac ctggccgatt
     3541 ggctgcggca agctgtttaa aggcgtttac cacctttata aagatgaaac ctatctttat
     3601 cagagcggta aaggccacac cattcaggaa gtccgtattg ttaaagggct gaataacccg
     3661 gatctcgacg ctgcggttgg tgaagatctg gcgcagcagc tgcgtgatga actggagctg
     3721 gtgaaaggcg cgtctaacga gttcgacaaa gaattgttcc ttgcgggcga aatcactccg
     3781 gtattctttg gtactgcgct gggtaacttc ggcgtcgatc atatgctgga tggtctggtg
     3841 gagtgggcac ctgcgccgat gccgcgtcag actgataccc gtaccgtaga agcgagcgaa
     3901 gacaaattta ccggcttcgt atttaaaatt caggccaaca tggacccgaa acaccgcgac
     3961 cgcgtggcgt ttatgcgcgt ggtatccggt aaatatgaaa aaggcatgaa actgcgccag
     4021 gtgcgcaccg cgaaagatgt ggtgatctcc gacgcgctga cctttatggc gggcgaccgt
     4081 tcgcacgttg aagaagcgta tccgggagac atcctcggcc tgcacaacca cggcaccatt
     4141 cagatcggcg acacctttac ccagggtgag atgatgaagt tcaccggtat tccgaacttc
     4201 gcaccagaac tgttccgtcg tatccgcctg aaagatccac tgaagcaaaa acagctgctc
     4261 aaagggctgg tacagctttc cgaagagggc gcagtgcagg tgttccgtcc gatctccaac
     4321 aacgacctga tcgttggtgc agttggtgtg ctgcagtttg atgtggtggt agcgcgcctg
     4381 aagagcgaat acaacgttga agcagtgtat gagtcagtca acgttgccac tgcacgctgg
     4441 gtagaatgtg cggacgcgaa gaaattcgaa gagttcaagc gtaagaacga aagccaactg
     4501 gcgcttgatg gcggcgataa cctcgcttac atcgctacca gcatggtcaa cctgcgtctg
     4561 gcgcaggaac gttatccgga cgttcagttc caccagaccc gcgagcatta attcttgcct
     4621 ccagggcgcg gtagccgctg cgccctgtca atttcccttc cttattagcc gcttacggaa
     4681 tgttcttaaa acattcactt ttgcttatgt tttcgctgat atcccgagcg gtttcaaaat
     4741 tgtgatctat atttaacaaa gtaatgacat ttctgacggc gttaaatacc gttcaatgcg
     4801 tagatatcag tatctaaagc cgtcgattgt cattctaccg atattaataa ctgattcaga
     4861 ggctgtaatg gtcgttattc atcacccatc gcttttgtga tggcgaccat tgacttctgt
     4921 agagggtgaa gtctctccct attcagcaat gcaacctcgt gttgccaggc tcaaattacg
     4981 agcaaacata caggaataaa tcgatgacta tgacaagact gaagatttcg aaaactctgc
     5041 tggctgtaat gttgacctct gccgtcgcga ccggctctgc ctacgcggaa aacaacgcgc
     5101 agactaccaa tgaaagcgca gggcaaaaag tcgatagctc tatgaataaa gtcggtaatt
     5161 tcatggatga cagcgccatc accgcgaaag tgaaggcggc gctggtggat catgacaaca
     5221 ttaagagcac cgatatctct gtcaaaaccg atcaaaaagt cgtgaccctg agcggtttcg
     5281 ttgaaagcca ggctcaggcc gaagaggcag tgaaagtggc gaaaggcgtt gaaggggtga
     5341 cctctgtcag cgacaaactg cacgttcgcg acgctaaaga aggctcggtg aagggctacg
     5401 cgggtgacac cgccaccacc agtgaaatca aagccaaact gctggcggac gatatcgtcc
     5461 cttcccgtca tgtgaaagtt gaaaccaccg acggcgtggt tcagctctcc ggtaccgtcg
     5521 attcccaggc acagagcgac cgtgctgaaa gtatcgccaa agcggtagat ggtgtgaaaa
     5581 gcgttaaaaa tgatctgaaa actaagtaat tcgtcgtaat tcgtcctccc gaactttgtc
     5641 gggaggcgcg atgtgcacca cactaaaaat atcgcattga gtgattttca cgctcatatc
     5701 aagcggtgac attaactatg gtaaaggaga cgcttatgtt tcgttggggc atcatatttc
     5761 tggttatcgc gttaatcgcc gccgcacttg ggtttggtgg tctggccggt accgctgcag
     5821 gcgcagctaa aattgtcttt gtcgtcggga ttattctgtt cctggtgagt ttgttcatgg
     5881 gccgaaaacg accctagatt tcagaccatt aagaaaattt tatccaaagc cagtccagcg
     5941 gactggcttt tgcggtttta gcgaataata aattgcgtta ctttgtcatt ctttcacaac
     6001 aaaaacagga aggcagaggt ggggcagcga atacctgtaa cgcttggtaa tattgcgccg
     6061 ttgtcgctaa ggccgttcca gcctggacga atagctctgg tgtgcgaagg cggcggacag
     6121 cgtggaattt tcacggctgg cgtgctggat gagtttatgc gcgcgcagtt taatcctttc
     6181 gatctttatc tcggcacatc tgccggggcg cagaacctct cggcgtatat ctgcaatcag
     6241 cccggttacg cgcgcaaagt catcatgcgc tataccacaa aacgcgaatt tttcgatcca
     6301 ttgcgctttg tccgtggagg aaatcttatc gatctcgact ggctggtgga ggccactgca
     6361 agccagatgc cgttgcaaat ggataccgcc gcgcggttgt ttgacagcgg caaatcgttt
     6421 tatatgtgcg cctgtcgtca ggatgactat gcgccgaatt actttttacc aaccaaacaa
     6481 aactggctgg atgtgattcg cgcctccagt gcgatacctg gcttttatcg tagcggagtg
     6541 tcgctggaag gcattaacta cctggatggc gggatcagtg atgcgattcc ggttaaagag
     6601 gcggcaaggc agggagcaaa aaccttggtc gtcatccgca ccgtgccgtc gcaaatgtac
     6661 tacacgccgc agtggttcaa acgcatggaa cgctggctgg gtgacagtag cctgcagccg
     6721 ctggtcaatc tggtgcagca tcatgaaacc agctattgtg atattcagca atttattgag
     6781 aaaccaccgg gcaaactgcg gatattcgaa atttatccgc cgaagccatt gcatagtatc
     6841 gcgcttggca gtcggattcc ggcgctgcgt gaagactata aacttgggcg tttatgcggt
     6901 cgttatttcc tggcaacggt tggcaagcta ttaactgaaa aagcgccgct aacccgccat
     6961 ctggtgccag tggtgacgcc ggaatcgatt gtcattccgc ctgcgccagt cgccaacgat
     7021 acgctggttg ccgaagtgag cgatgctccg caggcgaacg acccgacatt taacaatgag
     7081 gatctggctt gatttgccgt tttatcgaca cccactgcca ttttgatttc ccgccgttta
     7141 gtggcgatga agaggccagc ctgcaacgcg cggcacaagc gggcgtaggc aagatcattg
     7201 ttccggcgac agaggcggaa aattttgccc gcgtgcaggc gttagcggaa aaatatcaac
     7261 cgctgtatgc cgcattgggc ttgcatcctg gtatgttgga aaaacatagc gatgtgtctc
     7321 ttgatcagct acagcaggcg ctggaaaggc gtccggcgaa ggtggtggcg gtgggggaga
     7381 tcggtctgga tctcttcggc gacgatccgc aatttgagag gcagcagtgg ttactcgacg
     7441 aacaactgaa actggcgaaa cgctacgatc tgccggtgat ccttcattca cggcgcacgc
     7501 acgacaaact cgcgatgcat cttaaacgcc acgatttgcc gcgtactggc gtggttcacg
     7561 gtttttccgg cagcctgcaa caggctgaac ggtttgtaca gctgggctac aaaattggcg
     7621 taggcggcac tatcacctat ccacgcgcca gtaaaacccg cgatgtcatc gcaaaactac
     7681 cgctggcatc gttgttgctg gaaaccgacg cgccggatat gccgctcaac ggctttcagg
     7741 ggaagcctaa tcgcccggaa caggccgcgc gggtgtttgc cgtgctttgc gagttacgcc
     7801 cggaacccgc ggatgaaatt gccgaagtgt tgcttaataa cacgtatacg ttgtttaacg
     7861 tgccgtaggc cggacaaggc gtttacgcca catccggcat gtcacaaata cagcgccgga
     7921 aatatcaacc ggctcacccc gcgcaccttt aacgcatcag ccaactgctc aacatcttcc
     7981 ggtgtggcgc tcggccagct ttgcgcttcg ccatacacgc cgtgggcatg aaacgcgttc
     8041 aggcgtaccg gaacatcgcc aagtaccttg ataaacgccg ccagttcttc gatgtgttgc
     8101 aaataatcca cctggccagg gattaccagc aaacgcagtt ccgccagctt gccgcgctct
     8161 gccagcaaat agatgctgcg cttaatctgc tgattatcgc gtccggttaa ctgttgatga
     8221 cattcgctcc cccacgcttt gagatcgagc atcgcgccgt cgcacaccgg gagcaatttt
     8281 tcccagccgg tttcgctcaa cataccgtta ctgtccacca gacaggtgag atggcacagt
     8341 tgcggatcat ttttgatagc agtaaacagc gccaccacaa acggtagctg ggtcgtggct
     8401 tcgccgccgc ttaccgttat cccttcgata aacagcactg ctttgcggat atggctaagc
     8461 acctcatcca cgctcatgga ctgcgccatt ggcgtggcat gttgcggaca catcttcagg
     8521 caggtatcac attgctcgca gaccacagcg ttccacacca ctttgccgtc aacaatctgc
     8581 aacgcctgat gcggacactg tggcacgcac tccccgcagt cattgcaacg tcccatcgtc
     8641 cacggattgt gacagttttt gcagcgcaga ttgcagccct gcaaaaacag agccagacga
     8701 ctgcctggcc cgtcaacgca ggagaagggg ataatcttac tgactaaagc gcatctgctg
     8761 ttcatggctt atcacgcgcg gctggcgttc cagaatacga gtgttacgtg cggcctcttc
     8821 gcccagccag gtggtgttgg tacgtgaacc ttcggcgcga tatttttcta aatccgacaa
     8881 acgcaccata taaccggtaa cgcgaaccag atcgttaccg ctgacattgg cggtaaattc
     8941 acgcattccg gctttaaagg caccgaggca aagctgtacc agcgcctgcg gattacgttt
     9001 gatggtttcg tcgagcgtca gaatgtcgct gatgccggaa taataataag catgatgcgg
     9061 ggcgacggtt tgcagatggg tgatcggatc tggctcatca ccatacggca aacgtgcgcc
     9121 cggcgtggtg ccgatatcgg aacttatccc tgactgtgcg tgtaacatgg cgcgtttttg
     9181 ccagccatat ttcacggggg tattggcgac aaactccgcc agttgcgcgc tgatgcgata
     9241 acccacttca tttacggcgg cttctttacc gtagcgcgcg gcaatccctt ctttctcaca
     9301 cagcaagtta accgcttccg ccagcccata catgccaaac attggcacaa aacgttcagg
     9361 gttaatcagc ccttctttca ccaggaagct attctcaaag aagtgtgatt gttgatagag
     9421 gaattcacac cgcgcatcga tgatggcgat ctgctgctgg cagtagtgcg gtagagtgcg
     9481 cgcaaagaag tcatccagcg attcgctgcg ctcggcaatg gctttcaggt taaggcgtac
     9541 cagcgtgctg ccaccacccg ccagcggcag tgagttgtaa cagctcacaa tcccgtagcc
     9601 cccttttgtg aaaattttat catgcaccgg accgttggcg atgtgcggtt tgctacattc
     9661 acagatgttc ttcgccactt cctgcagcag gtcatcaggg gtgatttcag gatcgtagat
     9721 aaaggtcagg ttcggtgaaa cctgcttcag ctctgcatct gcacgtaaga ttgcacgggt
     9781 gatgggcgaa tcagacgggc cgatattggc gtgcataaag gcgtctggca gggttctgtc
     9841 gaggtaacgc cagaaacgtt ttattcgaat atcgatctcg tcttgtgtta gaattctaac
     9901 atacggttgc aacaacgcat ccagttgccc caggtagacc ggcatcgatg tgaccgacgg
     9961 tacgtggtgg taaagaatgg tcagcagaga gagtgcatca tcaagatctt tcgcgccttc
    10021 cagctccagc cattcggaac cgttcgccag aaaacgggcg taatcgggta agacatagcg
    10081 cggtttgtac ggcgcatgac cttcaaacat atcgcagatt acaccctcat ccagcgcgcg
    10141 gcgggcttcg gcaggaagct gcggataagg cagattgttt tctgcttcca gcgccagaaa
    10201 atggcgcttc tgctccgggc taagtaccgg actggtgaca atttgctggc aacgttgttg
    10261 cagtgcaatc tcatgagaag tgggcatctt cttttccttt tatgccgaag gtgatgcgcc
    10321 attgtaagaa gtttcgtgat gttcactttg atcctgatgc gtttgccacc actgacgcat
    10381 tcatttgaaa gtgaattatt tgaaccagat cgcattacag tgatgcaaac ttgtaagtag
    10441 atttccttaa ttgtgatgta tatcgaagtg tgttgcggag tagatgttag aatactaaca
    10501 aactcgcaag gtgaatttta ttggcgacaa gccaggagaa tgaaatgact gatctgaaag
    10561 caagcagcct gcgtgcactg aaattgatgg acctgaccac cctgaatgac gacgacaccg
    10621 acgagaaagt aattgctctg tgtcatcagg ccaaaactcc ggtcggcaat accgccgcta
    10681 tctgtatcta tcctcgcttt atcccaattg ctcgcaaaac gctgaaagag cagggtaccc
    10741 cggaaatccg tatcgctacg gtaaccaact tcccacacgg taacgacgac atcgacatcg
    10801 cgctggcaga aacccgtgcg gcaatcgcct acggtgccga tgaagttgac gtagtgttcc
    10861 cgtaccgtgc gctgatggca ggtaacgagc aggttggttt tgacctggtg aaagcctgta
    10921 aagatgcttg tgcggcagcg aatgtactgc tgaaagtgat catcgaaacc ggcgaactga
    10981 aagacgaagc gctgatccgt aaagcgtctg aaatctccat caaagcgggt gctgacttca
    11041 tcaaaacctc taccggtaaa gtggcagtga acgcgacgcc ggaaagcgcg cgcatcatga
    11101 tggaagtgat ccgtgatatg ggcgtagaaa aaaccgttgg tttcaaaccg gcgggcggcg
    11161 tgcgtactgc ggaagatgcg cagaaatatc tcgccatcgc cgatgaactg ttcggcgctg
    11221 attgggccga tgcgcgtcac taccgctttg gcgcttccag cctgctggca agcctgctga
    11281 aggctctggg ccacggcgac ggtaagagcg ccagcagcta ctaaggcttg atttgccgga
    11341 tgacgcagcg atgctcatcc ggttccccga ttacctcgtc tcttttccac gggaggttac
    11401 cttgtttctc gcacaagaaa ttattcgtaa aaaacgtgat ggtcatgcgc tgagcgatga
    11461 agaaattcgt ttctttatca acggtattcg cgacaacact atctccgaag ggcagattgc
    11521 cgctctcgcg atgaccattt tcttccacga tatgacaatg cctgagcgtg tctcgctgac
    11581 catggcgatg cgagattcag gaaccgttct cgactggaaa agcctgcatc tgaatggccc
    11641 aattgttgat aaacactcca ccggtggcgt cggcgatgtg acttcgctga tgttggggcc
    11701 gatggtcgca gcctgcggcg gctatattcc gatgatctcc ggtcgcggcc tcggtcatac
    11761 tggcggtacg ctcgacaaac tggaatccat ccctggcttc gacattttcc cggatgacaa
    11821 ccgtttccgc gaaattatta aagacgtcgg cgtggcgatt atcggccaga ccagctcact
    11881 ggctccggcg gataaacgtt tctacgcgac ccgtgatatt accgcaaccg tggactccat
    11941 cccgctgatc accgcctcta ttctggcgaa gaaactggcg gaaggtctgg acgcgctggt
    12001 gatggacgtg aaagtgggta gcggcgcgtt tatgccgacc tacgaactct ctgaagccct
    12061 tgccgaagcg attgttggcg tggctaacgg cgctggcgtg cgcaccaccg cgctgctcac
    12121 cgatatgaat caggtactgg cctccagtgc aggtaacgcg gttgaagttc gtgaagcggt
    12181 gcagttcctg acgggtgagt atcgtaaccc gcgtctgttt gatgtcacga tggcgctgtg
    12241 cgtggagatg cttacctccg gcaaactggc gaaagatgac gccgaagcgc gcgcgaaatt
    12301 gcaggcggtg ctggacaacg gtaaagcggc agaagtcttt ggccgtatgg tggcggcaca
    12361 aaaaggcccg accgacttcg ttgagaacta cgcgaagtat ctgccgacag cgatgctgac
    12421 gaaagcagtc tatgctgata ccgaaggttt tgtcagtgaa atggataccc gtgcgctggg
    12481 gatggcagtg gttgcaatgg gcggcggacg ccgtcaggca tctgacacca tcgattacag
    12541 cgtcggcttt actgatatgg cgcgtctggg cgaccaggta gacggtcagc gtccgctggc
    12601 ggttatccac gcgaaagacg aaaacagctg gcaggaagcg gctaaagcgg tgaaagcggc
    12661 aattaaactt gccgataaag caccggaaag cacaccaact gtctatcgtc gtatcagtga
    12721 ataacggtat actgatctga tcatttaaat ttgaagcact gagtacggag aacatatgaa
    12781 acgtgcattt attatggtgc tggactcatt cggcatcggc gctacagaag atgcagaacg
    12841 ctttggtgac gtcggggctg acaccctggg tcatatcgca gaagcttgtg ccaaaggcga
    12901 agctgataac ggtcgtaagg gcccgctcaa tctgccaaat ctgacccgtc tggggctggc
    12961 gaaagcacac gaaggttcta ccggtttcat tccggcggga atggacggca acgctgaagt
    13021 tatcggcgcg tacgcatggg cgcacgaaat gtcatccggt aaagataccc cgtctggtca
    13081 ctgggaaatc gccggtgtgc cggttctgtt tgagtgggga tacttctccg atcacgaaaa
    13141 cagcttcccg caagagctgc tggataaact ggtcgaacgc gctaatctgc cgggttacct
    13201 cggtaactgc cactcttccg gtacggtcat tctggatcag ctgggcgaag agcacatgaa
    13261 aaccggcaag ccgattttct atacctccgc tgactccgtg ttccagattg cctgccatga
    13321 agaaactttc ggtctggata aactctacga actgtgcgaa atcgcccgtg aagagctgac
    13381 caacggcggc tacaacatcg gtcgtgttat cgctcgtccg tttatcggcg acaaagccgg
    13441 taacttccag cgtaccggta accgtcacga cctggccgtt gaaccgccag caccgaccgt
    13501 gctgcagaaa ctggttgatg aaaaacacgg ccaggtggtt tctgtcggta aaattgcaga
    13561 catctacgcc aactgcggta tcaccaaaaa agtgaaagcg actggcctgg acgcgctgtt
    13621 tgacgccacc atcaaagaga tgaaagaagc gggtgataac accatcgtct tcaccaactt
    13681 cgttgacttc gactcttcct ggggccaccg tcgcgacgtc gccggttatg ctgcgggtct
    13741 ggaactgttc gaccgccgtc tgccggagct gatgtctctg ctgcgcgatg acgacatcct
    13801 gatcctcacc gctgaccacg gctgtgatcc gacctggacc ggtactgacc acacgcgtga
    13861 acacattccg gtactggttt acggcccgaa agtaaaaccg ggctcactgg gtcaccgtga
    13921 aaccttcgcg gatatcggcc agactctggc aaaatatttt ggtacttctg atatggaata
    13981 tggcaaagcc atgttctgat ggatttgggc ggagcgttga ctccgccttt gttatgtcac
    14041 aaaaaggata aaacaatggc taccccacac attaatgcag aaatgggcga tttcgctgac
    14101 gtagttttga tgccaggcga cccgctgcgt gcgaagtata ttgctgaaac tttccttgaa
    14161 gatgcccgtg aagtgaacaa cgttcgcggt atgctgggct tcaccggtac ttacaaaggc
    14221 cgcaaaattt ccgtaatggg tcacggtatg ggtatcccgt cctgctccat ctacaccaaa
    14281 gaactgatca ccgatttcgg cgtgaagaaa attatccgcg taggctcctg tggcgctgtt
    14341 ctgccacacg taaaactgcg cgacgtcgtt atcggtatgg gtgcctgcac cgattccaaa
    14401 gttaaccgca tccgttttaa agaccatgac tttgccgcta tcgctgactt tgacatggtg
    14461 cgtaacgcag tagatgcagc taaagcactg ggcattgatg ctcgcgttgg taacctgttc
    14521 tccgctgacc tgttctactc tccggacggc gaaatgttcg acgtgatgga aaaatacggc
    14581 atcctcggcg tggaaatgga agcggctggt atctacggcg tcgctgcaga atttggcgcg
    14641 aaagccctga ccatctgcac cgtgtctgac cacatccgta ctcacgagca gaccactgcc
    14701 gctgagcgtc agaccacctt caacgacatg atcaaaatcg cactggaatc cgttctgctg
    14761 ggcgataaag agtaattgtg tttcgctgaa aggcgattgc cttgtgaagc cggagcggga
    14821 gactgctccg gctttttagt atctattcat caatagcaac tgatttatag atttactatt
    14881 atttttcata tggatagaaa ataaaataca ttcaagtatc tattcacgaa tctattcatt
    14941 tatgttcagc cgtcatccag gaagggcata tcagcaactc tggtggacaa ccataaattc
    15001 ctgccatttt gcttttgatg tttgaactaa gccgttccgt cttttcgatc tgttttagag
    15061 aattaatgga gatacctaac attttactta cttcagcaag tgatagttta tgcctgatac
    15121 gcacggcagt tatccaggat ataccctggc tgaacattaa attgataatc tcccgttcct
    15181 ggttttccag atcgatcata tttccttccc tggatatttt aatcagcgag ttaccttacc
    15241 gccccagcca tccatgccga taactcccgt agctcttttt cctgttccgg gaagtcaacc
    15301 aacagcgctt cgcactcctg ttgcagcata tctgcgcggt acaggcagcc ttgcagtcgc
    15361 ccggcgaggg cttccagcgg cgcggggttg aggctgtcgg taaacacctg ggcgcgggtg
    15421 atatggcctt tttcaacgtc gaaatgcagt tccacaccgc cccaggtaaa gcgttcatcc
    15481 agcagatgcg agaatgccgg agcctgaccg aagttccatt cccagctact ctggcgggca
    15541 aaggtttcgg cgaagttcgg caggtctggc gttttgtccg gggagatgat ttccgcttcc
    15601 acgcgctcgc cataatgggc gaaaaaggcc tcggttatgg cctcgcaaac ctgctcatgg
    15661 gtaatttccg gcagcaattc ggtgaggttg gtcacgcggg aacgtaccga cgtaatgcct
    15721 ttcgccgcca gtttcttttt atccggattg agatagtttg ccaggcggct aaggtcggca
    15781 ttgagcagca aggtgccgtg gtggaagcca cgatctttgg tttcgcgata ggccgagcct
    15841 gagactttgc ggtcgccttc ggcggttttc accaccagat cgttacgtcc ggacgcttcg
    15901 gcgctgacgc cgagcgcgtt cagtgcattg agcacaatcg acgtggagat ggttttatcg
    15961 tactccggct tgccagccat aaaggtaaaa caggtattgc cgagatcgtg gaacaccgcg
    16021 ccgccaccgc tactgcgccg cgccaggcgg acgttatctt cttccatccg ccgggtatta
    16081 cactctttcc acgggttctg tgcgcgacca atcactaccg tgtcggcatt gcgccagaga
    16141 aacagaacgc gctgcgtggc gggcatctgg cgaaaaatac actcctccac cgccaggtta
    16201 aaccacgggt cgtaagagtc agagatgagc aggcgtaatg tggacataac gatttccttt
    16261 cttgtagtaa tgggtcactc ttttttctcg ctttcttcct cttccggcac cggtttgctg
    16321 gcggttaaca ggaagggcga ttgctgccag cgggtgcgtt taccctgtag cagcgtgcgg
    16381 gtcagtacta cgccgattgc cagtgagagc agcaacatca ggcgtaaaat gttagtggtg
    16441 ttatccacct gttgggcttc ggtggcgagc gtatgggtgt cgagcgtcag gcgcagatag
    16501 ccgagcggtc cgtttttccc ggcaataggt tcgacaatct gctggttaaa atagccgccc
    16561 gcttttttgc cgtcgagcgc caaccggtcg cgcacttcga cgctttcgcc agaacgggcg
    16621 ataagatcgc cttgttcgtc atacacgccc gcgtcgagga tgcggctttc atccgttaac
    16681 tgatcgagga tcgcctgaat gcgtttttca tccggtgagt cgttacgcat cagcggtgca
    16741 acgttcagcg tcacctgacg cgccagggtg cgggccagtt cttccagctg tggattacgc
    16801 tgtcgctggt ggttttgact aaaccatgac gctccctgca tcagcgccac taacaaggca
    16861 agacagaaca ggacaatcac tgcccgatgc agccggaatt tcagttttgt gcgagccatc
    16921 ttccaccctt tgaaaatttg agacttaatg ttgccagaag caatggatac aaggtagcct
    16981 catgcgttat tttccctgct tcgaacgatt ttacaggagc cttaatgcct aacattacct
    17041 ggtgcgacct gcctgaagat gtctctttat ggccgggtct gcctctttca ttaagtggtg
    17101 atgaagtgat gccactggat taccacgcag gtcgtagcgg ctggctgctg tatggtcgtg
    17161 ggctggataa acagcgtctg acccaatacc agagcaaact gggcgcggcg atggtgattg
    17221 ttgccgcctg gtgcgtggaa gattatcagg tgattcgtct ggcaggttca ctcaccgcgc
    17281 gggctacgcg cctggcccac gaagcgcagc tggatgtcgc gccgctgggg aaaatcccgc
    17341 acctgcgcac gccgggtttg ctggtgatgg acatggattc caccgccatc cagattgaat
    17401 gtattgatga aattgccaaa ctggccggaa cgggcgagat ggtggcggag gtaaccgaac
    17461 gggcgatgcg cggcgaactc gattttaccg ccagcctgcg cagccgcgtg gcgacgctga
    17521 aaggcgctga tgccaatatt ctgcaacagg tgcgtgaaaa tctgccgctg atgccaggct
    17581 taacgcaact ggtgctcaag ctggaaacgc tgggctggaa agtggcgatt gcttccggcg
    17641 gctttacttt ctttgctgaa tatctgcgcg acaagctgcg cctgacagcc gtggtagcca
    17701 atgaactgga gatcatggat ggtaaattta ccggcaatgt gatcggcgac atcgttgacg
    17761 cgcagtacaa agcgaaaact ctgactcgcc tcgcgcagga gtatgaaatc ccgctggcgc
    17821 agaccgtggc gattggtgat ggagccaatg acctgccgat gatcaaagcg gcagggctgg
    17881 ggattgccta ccatgccaag ccaaaagtga atgaaaagac ggaagtcacc atccgtcacg
    17941 ctgacctgat gggggtattc tgcatcctct ctggcagcct gaatcagaag taattgctcg
    18001 cccgtcatcc tgcgggcggc acagcattaa cgaggtacac cgtggcaaaa gctccaaaac
    18061 gcgcctttgt ttgtaatgaa tgcggggccg attatccgcg ctggcagggg cagtgcagcg
    18121 cctgtcatgc ctggaacacc atcaccgagg tgcgtcttgc agcgtcgcca acggtggcgc
    18181 gtaacgagcg tctcagtggc tatgccggta gcgccggggt ggcaaaagtc cagaaactct
    18241 ccgatatcag ccttgaagag ctgccgcgtt tttccaccgg atttaaagag ttcgaccgcg
    18301 tactaggcgg tggcgtggtg ccgggaagtg ccattctgat tggcggtaac ccaggtgccg
    18361 ggaaatccac attgctattg caaacgctgt gcaaactggc ccagcagatg aaaacgctgt
    18421 atgtcactgg cgaagagtcg ctgcaacagg tggcaatgcg cgctcatcgc cttggcctgc
    18481 cgaccgacaa tctcaatatg ttgtcggaaa ccagcatcga gcagatctgc ctgattgccg
    18541 aagaagagca accgaagctg atggtaattg actccatcca ggtgatgcat atggcggatg
    18601 ttcagtcatc gcctggcagt gtggcgcagg tgcgtgaaac ggcggcttat ctgacgcgct
    18661 tcgccaaaac gcgcggcgtg gcgattgtca tggttgggca cgtaaccaaa gatggctcgc
    18721 tggctggccc taaagtgctg gaacactgta tcgactgttc ggtgcttctg gatggcgatg
    18781 ccgactcccg ttttcgcacc ttacgcagcc ataaaaaccg cttcggcgcg gtgaatgagc
    18841 tgggcgtctt cgcgatgacc gaacaggggc tgcgtgaagt cagcaaccct tctgccatct
    18901 ttttaagccg cggtgatgaa gtgacctccg gtagctcagt gatggtagtg tgggaaggaa
    18961 cgcgtccgct gctggtggag atccaggcgc tggtcgatca ctcgatgatg gcgaatccac
    19021 gccgcgtcgc agtagggctg gagcaaaacc gtctggcaat cctgctggcg gtgctgcacc
    19081 gtcacggtgg gttgcaaatg gccgatcagg atgtatttgt taacgtggtc ggcggcgtga
    19141 aggtgacgga aaccagcgcc gacttagcgt tactgctggc gatggtttcc agcctgcgcg
    19201 acagaccgct gccacaggat ctggtggtgt ttggtgaagt cgggctggca ggggagatcc
    19261 gcccggtgcc cagcggccag gaacgaatct ctgaagcggc gaaacacggt tttcgccggg
    19321 cgattgttcc ggcggctaat gtgccgaaaa aagcgccgga agggatgcag atttttggcg
    19381 ttaaaaaact ctccgacgcg cttagcgtgt tcgacgactt ataatgagag atacggaggg
    19441 agatatgtcg tcatttgatt acctgaaaac tgccatcaag caacagggct gcacgctaca
    19501 gcaggtagct gatgccagcg gtatgaccaa agggtattta agccagttac tgaatgccaa
    19561 aattaaaagc cccagcgcgc aaaagctgga ggcgttgcac cgttttttgg ggcttgaatt
    19621 tccccggcag aagaaaacca ttggtgtcgt attcggtaag ttctacccgc tgcataccgg
    19681 acatatctac cttatccagc gcgcctgtag ccaggttgac gagctgcata tcattatggg
    19741 ttttgacgat acccgcgacc gcgcgttgtt tgaagacagc gccatgtcgc agcagcccac
    19801 cgtgccggat cgtctgcgct ggttattgca aacctttaaa tatcagaaaa atatccgtat
    19861 tcatgctttc aacgaagagg gcatggagcc atatccgcac ggctgggatg tgtggagcaa
    19921 cggcatcaaa aagtttatgg ctgaaaaggg gatccagccg gacctgatct acacctcgga
    19981 agaagccgat gcgccacagt atatggaaca tctggggatc gagacggtgc tggtcgatcc
    20041 gaagcgtacc tttatgagta tcagcggtgc gcagatccgc gaaaacccgt tccgctactg
    20101 ggaatatatt cccaccgaag tgaagccgtt ctttgtgcgt accgtggcga tccttggcgg
    20161 cgagtcgagc ggtaaatcca ccctggtaaa caaacttgcc aatatcttca ataccaccag
    20221 tgcgtgggaa tatggtcgcg attatgtctt ttcacacctc ggcggtgatg agatcgcatt
    20281 gcagtattct gactacgata aaatcgcgct gggacacgct caatatattg attttgcagt
    20341 gaaatatgcc aataaagtgg cgtttattga cactgatttt gtcactaccc aggcgttctg
    20401 caaaaagtac gaagggcggg agcatccgtt cgtgcaggcg ctgatcgatg aataccgttt
    20461 tgatctggtt atcttgctgg agaacaacac cccgtgggtg gcggatggtt tacgcagcct
    20521 cggtagttcg gtggatcgca aagagttcca gaacttgctg gtggagatgc tggaagagaa
    20581 caatatcgaa ttcgtgcggg ttgaagagga cgattatgac agccgtttcc tgcgctgcgt
    20641 ggagctagtg cgggagatga tgggggagca gagataaccg tgacgaaacg gctcaaaggc
    20701 gaggtataaa ataagttttt tgatgagttg aaaacctctc tggaagagac tgtcgagatt
    20761 agaaagaacc tgtacgggtg acccgctacg aaattgcgga tgctaaggct attcgtgagc
    20821 aacttcatac tccaaccgac cggttgcgta gccagaatgc aatcaaatct gacagtctgc
    20881 ttcggcgcgc gctttggtgt atttaccgga gtaaatcagc gtgccgtgga aaattatgcg
    20941 cagcgcttct gctgatttgc gtaccttcgc tgatgataag tcctgcactg gcgcgctgcg
    21001 cgtaatagtc cgccattatg tcagtcgcaa cgtcacccgc gcgggaacgc gtcatcggtg
    21061 gcattacaat acggtttatt aatggcaacg cattcagcgc atatttagca aattacgtaa
    21121 tgggtccctc gcgcgattat ttatacagtt cggcactcat ggtgacggta ttgcccatgc
    21181 gagcgctggt aattttatat tcagaggcgt tcatctcttt cgctttctgc gcaataactg
    21241 cttccgcaga atccagtgtt gtaccggttg ctgtcacgct ttgcgccgat gcgttaaacg
    21301 ccgcgacaga gaaaagagaa gctgtaaaaa ttgcgatgat cgttttcatg tttatattcc
    21361 agagtgtctt aattgtttgg cgtttattaa cgcgatgggg agagaatacg cctgctgcct
    21421 gataacataa atgatacaaa tgacaataga ctattctgat tttgttaata atcagacgcc
    21481 ggacgttccg acgcctgtcg atcgcatcag gcaacaaagt tgtccttcag cgccttgaga
    21541 ccatcccgat agatgccggt aaacagcgct tcggcctctt cgttcgtcac attcaccggg
    21601 gtgaattcac cgaaccattc cacacgggaa acctggctgt cggcggtttc atgaacggta
    21661 atggtggaga ggtaatccac caccgggaac ggtgcctgaa taatggaata ggtataactg
    21721 cgttgacggt tatcaaacgc ttccagacgt tcaaccacag tgccgccgtc agaggttgtc
    21781 agcgaacgaa cgcgaccgcc ttctgtcact acgcttttcg ggatgaacgg cagccagtct
    21841 ggcagcgcat caaagccgcc catcaattgc catacggcgt cggcagaagc cggaatttcg
    21901 atcgatacag ttgttttagc catgataatt ttctcttaat ttatagggtt acatagtcag
    21961 ggtgtcgacg acaccgccat cgacgcgcag ggctgcccct gtggttgccg aggaaagcgg
    22021 tgacgcgaga taaaccgcca gattcgctac ttcttccaca tccgccgcac gctggatgat
    22081 ggagctcgga cgcgaggttt taacaaactc atcggcctgt tcacgcgcac tgcgtcccga
    22141 ttgtgcagcg gcatccgcca gcatctcttt caggccatcg gtaaaggttg gccctggcag
    22201 aatggcgttg acggtcacac cggtccccgc cagtcgtttc gccagaccat gagagaccgc
    22261 cagattcgca gctttggtga cgccgtagtt gatcatgtcg ccaggaattg ccacgccgga
    22321 ttcggacgag acaaagataa tccgccccca gccctgttcc gtcatgcctg gcgcatagtg
    22381 acgcgccaga cgaacgccgg agaggacgtt gacgttatag aagtgcatcc actcatcatc
    22441 cggaacgcta aagaaatctt tatcgttgaa aatgcccaga ttattgacca gcacatcagc
    22501 cgttgggact gcagcgatca gcgcagccac gccgtcttcg gtgctgagat cggccaccac
    22561 gccacgcagt gacgccgtgg gagtttgtgt cgcaatcgct gccatggcgt catcaacgcc
    22621 gcgctgttta cggccgacga caaccaccgt tgcgcctgct tttgccagtc cggcggcgat
    22681 accctgccca atgccggagg ttgagccgct gaccaccgcg actttacctg tcagatccag
    22741 tttcatcgtt ttctcaccag gttgattaac gggtcggcag cggagcgttt tccgcaacca
    22801 gattttgacg gcgcatttct gcccagaatt cagccggaac aggcgcattc agtgcagcca
    22861 gatcttctgc catacggcca ggacggctgg agccaggaat aaccgctgca acggccgggt
    22921 ttgccagcgc gaactgcagc gcagctgctt taacgttcac attgaaacgt gtggcaatgg
    22981 cgttaatttg cgccactttc tgctggatgg ccgcagaggc tttctgatat tcaaagtgct
    23041 cgccgcctgc cagtacacca gagctgtacg gaccgcccac aacaatatcc acgttctgtt
    23101 ccagtgcttc tggcatcagg cgttgcagcg cacgttcgtg atccagcagg gaataacgcc
    23161 ccgccagcag gaacgcatcc ggcttcggtt cgtccagcgc cagcgtcagt tcgcaaggtt
    23221 cgacgcggtt cacacccagt ccccaggctt taatcacacc ttcgtcacgc aaacgtgtca
    23281 gtgcacgaaa cgcaccggta cgggcaatgt tgaactgctc cagccagctg tcaccgtaga
    23341 agtcctgcgc cgggtcgtgg atccagacaa tgtccagacg gtctgttttc aggcgtttca
    23401 ggctgtcttc aatcgagcgc agcgtggcgt cagcagagta gtcattcaga attttgtttt
    23461 tcaggccgtg ttcgaacagg ccaccttttt caccgaaatc ccgttttgcc ggatcttcca
    23521 tttcgtccag catgatgcga ccgactttgc tactcagaac gtattcatca cgtggatact
    23581 gagacagcgc ttcgcccatg cggatttcag aaaggccaga gccgtacagc ggcgcggtat
    23641 caaagtaacg cacgcccagg ttccaggctt cgctcacggt ggcgtgggcc tcttcttccg
    23701 gaatggcacg gaacatgttg ccgagtggtg cgccgcctaa gccaattttg ccgggaagta
    23761 aatttttgat gctcataaga tgtcctcaat tattcggttt gggttagggc gtttttttgc
    23821 tatcgccctg tgacgtggat cacaatacct tgcgaggttg tgacttgata ggacataatt
    23881 cgtctgactt aagtcctttg aggtctaaca tgattgattt acgccaactg aagtacttcg
    23941 tggtggtcgc agaagaagag cacgtgggac gcgcagccga gcgattacat atctcgcaat
    24001 caccgctgag ccgccagatt gcacaactgg aagaacggct ggggctgacg ctgtttgagc
    24061 gtaatcagca gcgcattcgc ctgaccgctg atggacagac attccttgcc gaaacacgtt
    24121 cgttgctgac acacgccaac cgcctggagt cgttaggcaa acgcctggga cggggggaag
    24181 atgggggctt gtgtattggc tatatcgaaa acgccatgca ttccggtgta ctttctacgg
    24241 cgttacgtac gttacgtgta tcgcgtccga atgtgcatat cgctctttac agccaaccac
    24301 cggcgaccca gattgaaggc ctgcgccagc gcagtctcga tttttctctg gtcgatacgc
    24361 cgcccaccga cagcgagcag gatctggaat atattcaggt tctggatgat cgaatgttgt
    24421 tggccttacc gcaaaatcat cctctggcgc aggaggaaaa ccttgtcgcg tcacaacttg
    24481 gcgatgagca gtggattgcg gtgattcata acgacgatgc gcattctcgc gatcattttg
    24541 tcgccgcctg tgttaacgcc gggtttaatc ctgatattcg cctggaagcg ggtgaacccc
    24601 tcacggcatt aggtttggtc gctgcgggat taggtctggc cttaattcaa catagtctgc
    24661 gtcataatgc gccggaaggg gtggtattac gtgaattgcc gtggatgaat tattcaacgc
    24721 aattatgggc agcctggcat aaagccaacc aacgtccgct ggtttcccat ttccgccaga
    24781 ttttgcgcgg tgaataaaac gttaagcggg aaccatgagt tcccgcttaa tttgagatgt
    24841 cagtccgcat ctgagctaat acgcttaaag aaaattctgc cttttccggg gactaatcat
    24901 ttaactaata ccggatgcga tgctggcgcg tcgcatccgg cattttacgc attacttcgc
    24961 aatacgcttg tacttgatac gcttcggctc cagcgcgtct gcgcccagcg tgcgtttctt
    25021 gtactcttcg tactcggtaa agttaccttc gaagaactca actttacctt catcctggta
    25081 gtccaggatg tgcgtggcga tacggtcgag gaaccaacgg tcgtgcgaga taaccatcgc
    25141 gcagcccggg aactccagca gggcgttttc cagcgcacgc agggtttcga tatccaggtc
    25201 gttggtcggt tcgtcgagca gcagcatgtt gccgccaact tgcagcagct tcgccagatg
    25261 cagacgaccg cgctcaccgc cggagagttc accaacgcgt ttaccctgat caaccccttt
    25321 aaagttaaag cggccaacgt aggcacggct tggcatctcg gtgttgccga tcttcatgat
    25381 atccagcccg ccggaaactt cttcccaaac ggttttgctg ttatccattg agtcacggaa
    25441 ctgatcaacc gatgctagtt tcaccgtttc acctaaagtg atggtgccgc tgtccggctg
    25501 ttcctgaccg gagatcatac ggaacagggt cgatttaccc gcaccgttcg gaccgatgat
    25561 cccgacgatc gctcctttcg ggatcgagaa gctcaggtca tcaatcagca gacgatcgcc
    25621 ataggattta cgcaggttgt tgacttccag cactttatcg ccaagacgcg gtcccggtgg
    25681 aataaacagt tcgttggttt cgttacgttt ctgatattcg gtgctgttca gctcttcaaa
    25741 acgcgccaga cgtgctttac ctttcgactg acggccttta gtcccctgac gtacccactc
    25801 cagctctttc tcaatcgact tacgacgcgc agcttcttgt gaagcttcct gcgccagacg
    25861 ttgatctttc tgctccagcc aggaggagta gttaccttcc cacggaatac cttcaccacg
    25921 gtccagttcg aggatccagc ccgccacgtt atcgaggaag taacggtcgt gggtaatcgc
    25981 cacaacagtg ccttcgaagt cgtgcaggaa gcgttccagc cacgccacgg attcggcatc
    26041 caggtggttc gtcggttcgt cgagcagcag catgtctggt ttttccagca gcaggcggca
    26101 caacgccacg cgacgacgct caccaccgga gaggttagcg attttcgcgt cccagtccgg
    26161 cagacgcagc gcatccgccg cacgctccag ctgaacgttc aggttgtgac cgtcgtgagc
    26221 ctgaatgatc tcttccagac ggccttgttc agcggccagc ttgtcaaaat cggcatccgg
    26281 atcggcgtac agcgcataca cttcatccag gcgtttcagg gcgttaacca cttcagaaac
    26341 cgcttcttca atggactcac gcacggtgtg ttccgggttc agctgcggtt cctgcggcag
    26401 ataaccaatc ttgatatccg gttgcggacg cgcttcgcct tcgatgtctt tatcaatgcc
    26461 cgccataatg cgcagcaggg tggacttacc cgcgccgttc agacccagga caccaatttt
    26521 tgccccaggg aagaaactca gagagatgtt tttcaaaata tgacgtttcg gcggaacaac
    26581 tttgccgaca cgatgcatgg tataaacgaa ttgagccacg ttggacttcg cctctatgtt
    26641 tatcgtgatg atgagttttc aaaggcgaag tgtagccttt ttccccgcct aatcccagcc
    26701 ggtcgatcac cctcgcagta aaagtaaaaa agtgtccgta acgtggcgta aacggcaatg
    26761 actggttagc ataaatctat tacgcggcat gacgctgcat tgatgtattt acacttagag
    26821 gatgcgcttg tggaaaaagc caaacaagtt acctggcggc tgttggctgc cggtgtctgt
    26881 ctgctgacgg tcagcagcgt ggcgcgagcc gactcactgg atgagcagcg tagccgctac
    26941 gcccaaatca agcaggcctg ggataatcga caaatggatg tggtcgaaca aatgatgcct
    27001 ggactgaagg attatccgct ttatccctac ctggaatacc gtcagatcac cgatgacctg
    27061 atgaatcaac cggcggtgac ggtcactaac tttgttcgcg ctaaccccac gcttcctccc
    27121 gctcgcacgc tgcaatctcg tttcgtcaat gaactggcgc ggcgtgaaga ctggcgtggc
    27181 ttgttagcct ttagcccgga aaagcccgga actaccgaag cgcaatgtaa ttactactat
    27241 gcgaaatgga ataccgggca gagtgaagaa gcctggcaag gggcgaaaga gctgtggcta
    27301 accggcaaga gccagcctaa cgcctgtgac aagttattta gcgtctggcg tgcgtcaggt
    27361 aaacaagatc cgctggcgta tttagagcgt atccgtttgg cgatgaaagc gggtaacact
    27421 ggactggtaa cagtgctggc agggcagatg cctgccgatt accagactat cgcctcggca
    27481 atcatttcac tggcgaacaa ccctaatacg gtactgacct tcgcgcgtac aaccggagcg
    27541 accgatttta cccgtcaaat ggcggcggtg gcgtttgcca gtgtggcgcg gcaggatgct
    27601 gagaatgcac ggctgatgat cccatcgctt gcccaggcgc agcagcttaa tgaagatcag
    27661 attcaggagc tgcgcgatat cgtcgcctgg cgtttgatgg gcaacgatgt caccgacgag
    27721 caggcgaaat ggcgcgatga cgccattatg cgctcgcaat ccacgtcgtt aattgaacgc
    27781 cgtgtgcgaa tggcgcttgg caccggcgat cgtcgcggcc tgaatacctg gctggcgcgt
    27841 ttgccgatgg aggcgaaaga gaaagatgaa tggcgttact ggcaggcgga tttactgctg
    27901 gaacgcggac gtgaagctga agcaaaagag attttgcatc aactcatgca acagcgtggt
    27961 ttctacccga tggttgcagc acaacgcatc ggagaagagt atgagctgaa gattgataaa
    28021 gcgccgcaga atgttgacag cgccctgact caggggccgg agatggcgcg cgtgcgcgag
    28081 ttgatgtact ggaatctcga taacaccgcg cgtagcgagt gggccaatct ggtgaagagc
    28141 aagtcaaaaa cagagcaggc gcaactggct cggtatgctt tcaataacca atggtgggat
    28201 cttagcgttc aggcaacgat cgccgggaag ctttgggatc atctggaaga acgattcccg
    28261 ctggcctaca acgatctttt caaacgttac accagtggga aggagatccc gcaaagctat
    28321 gcgatggcga ttgcccgtca ggagagtgcc tggaatccga aagtgaaatc gccggtaggg
    28381 gccagcggcc tgatgcagat tatgccgggc acggcgaccc atacggtgaa gatgttctct
    28441 attcctggtt atagcagccc aggacaattg ctggatccgg aaacgaacat caacattggc
    28501 accagttacc tgcaatatgt ttatcagcag tttggtaata accgtatttt ctcctcagca
    28561 gcttataacg ccggaccggg gcgggtgcga acctggcttg gcaacagcgc cgggcgtatc
    28621 gacgcagtgg catttgtcga gagtattccg ttctccgaga cgcgcggtta tgtgaagaac
    28681 gtgctggctt atgacgctta ctaccgctat ttcatggggg ataaaccgac attgatgagc
    28741 gccacggaat ggggacgtcg ttactgatcc gcacgtttat gatatgctat cgtactcttt
    28801 agcgagtaca accgggggag gcattttgct tcccccgcta acaatggcga catattatgg
    28861 cccaacaatc accctattca gcagcgatgg cagaacagcg tcaccaggag tggttacgtt
    28921 ttgtcgacct gcttaagaat gcctaccaaa acgatctcca tttaccgttg ttaaacctga
    28981 tgctgacgcc agatgagcgc gaagcgttgg ggactcgcgt gcgtattgtc gaagagttgt
    29041 tgcgcggcga aatgagccag cgtgagttaa aaaatgaact cggcgcgggc atcgcgacga
    29101 ttacgcgtgg atctaacagc ctgaaagccg cgcccgtcga gctgcgccag tggctggaag
    29161 aggtgttgct gaaagatcac tgatacaccg cattatgaaa cggactcagc gccaggatca
    29221 ccgcctggtg atagacgctg gcgcgggtaa gtttcccggc ggtaaacacg ccgatcgccc
    29281 cttccttacg accaatctca tcaataccgg tatagcgcga catcacggga ccgagcgcct
    29341 cgccttcacg cactttttcc agaatcaccg caggtaacgg caaagtagcc gaacgcgcct
    29401 cgccgcgctg gctggcgttt tcaatcacca cccaactgaa ggtgctgtcg ccatcaatgc
    29461 cagcttcaat cgccacccaa aaatcagcct ctggatgtaa acggcgggca tttgctaccc
    29521 gatttcgtgc gccagcgcgc gtttcctcac tgccaaaggg ctgttccggt acaccgctct
    29581 cgacggcaac ggatgcaata tggcaggatc cttcgccgaa gatctcgtga aatgcctgca
    29641 gaatggcctg aattttagcg ggattggtgg tcgcacagac aacttggtgc ataatcagca
    29701 ttactcagaa aattaacgtt acagcagtat acggaaaaaa agcatgttac aggtatacct
    29761 agtccgccac ggtgaaacgc agtggaacgc cgagcgacgt attcagggcc agtctgacag
    29821 cccgctgacc gccaaaggtg agcaacaggc gatgcaggtg gcaacccgtg ccaaagagct
    29881 tggcattacg catatcatca gtagcgattt aggacgcacc cggcgtacgg cggaaatcat
    29941 cgcccaggcc tgcggctgtg acatcatctt tgattctcgc ctgcgtgaat taaacatggg
    30001 tgtgctggaa aaaagacata tcgattctct gaccgaagaa gaagagaact ggcgtcggca
    30061 gctggtcaat ggcaccgttg acgggcgtat tcctgaaggc gagtcaatgc aggagctcag
    30121 cgatcgcgtc aatgcggcac tggaatcttg ccgggactta ccgcagggaa gccgaccgct
    30181 gctggtgagc cacggtattg cactgggatg cctggtgagt acgattctcg gattaccagc
    30241 atgggcagag cgtcgcttac gtctgcgtaa ctgttctatt tcgcgcgtgg attatcagga
    30301 aagcctgtgg ctggcgtctg gttgggtcgt tgaaaccgca ggggacatct cgcatctgga
    30361 cgcccctgca ttagatgagc tgcagcgtta acgacggatc ggaatcagca gttcacagcg
    30421 cagattaatt gggcgatctc ccgctttggc atcttctgcc gggtagtatc gctcaatatc
    30481 ctgaccttta cggcgcgtca ggttgagcat tggcatacac gttccgtata ccgtcaggat
    30541 aaactcctgc acgccggttc ccagaccttc ataggtaaac atcacatatt cgccgccctg
    30601 cagcatcacc ggatgccccg tcagtacata gccatctgcc tgatcctggg ctaacgcggt
    30661 ggtatagaat acttcttgtt cgtcgtcttt atcctgactc ggacgcgttt cattcaggcc
    30721 gtagagcacc ggcggaatgg tcggcgcgtt gccgagaaaa tcgtgccaga actgataacg
    30781 catttcatgg cggaaatcag agatttgctc cagcgaacag gagtagctct gggtaacacc
    30841 aatcagcggc gtatcttcca gggtgacaaa tttgtgctct ggcatagtga attcacccag
    30901 gcgtagcggc gggcgaatac caaaggcgct ccattcagga gaacggcggt aaagtgcagg
    30961 agtctgggca aactgcttct tgaatgcgcg ggtaaatgtc tgttgagagt cgaagcggta
    31021 ttgcagcgcg atgtccagaa tcggacgcgc agtcaggcgt agtgcgaccg ccgatttcga
    31081 caaacgacga gcacgaatat acgcgccaat agcatggcca gtgacatctt taaacattct
    31141 ctgtaagtgc cacttggaat aacctgcttt cgccgctaca ttgtcgagcg acaggggctg
    31201 atccagatga ccttccagcc agattaaaag gtcgcgaata atgccggcct gatccataaa
    31261 atatcctcat cctttcaaca acgagcacct gacatcaggt aattggataa tagcattttt
    31321 tgctgtttta gcattcagtg tttttttctt agtagagtat gttttaggac ttgacggaaa
    31381 taaaagtatt gagattttgt tcttaatcaa tatgttattt accgtgacga actaattgct
    31441 cgtgtaatag ataaaaatgg taacaatatg aaatacaagc atttgatcct gtctttaagc
    31501 ctgataatgc tggggccatt ggctcatgca gaagagattg gttcggtcga caccgtattt
    31561 aaaatgatcg gcccggatca caaaattgtt gtggaagcct ttgacgatcc cgatgtgaaa
    31621 aatgtcacct gttatgtgag ccgggcgaaa accggtggta ttaaaggggg attgggcctg
    31681 gcagaagata cctctgatgc ggccatttct tgtcagcaag tcgggccgat tgaactgtcg
    31741 gatcgtatta aaaacggcaa agctcagggc gaggtagtat tcaaaaaacg cacgtccctg
    31801 gtctttaagt cgttacaggt cgtgcgcttt tatgatgcca aacgcaacgc gctcgcttat
    31861 ctggcttact ccgacaaagt tgtagaaggt tcgccgaaaa acgcgattag cgcggttcct
    31921 gtcatgccgt ggcggcaata acagaggcga tttatgcaac gggaaacggt ctggttagtg
    31981 gaagatgagc aagggatagc cgacacgctg gtctacatgt tgcagcagga aggttttgcc
    32041 gtcgaggtct ttgagcgagg cttgccggtg ctggataaag ctcgccagca ggtacccgac
    32101 gtcatgattc tcgatgttgg tctgccggat attagcggct ttgaattgtg ccgccagtta
    32161 ctggcgctcc atccggcgtt acctgtactg ttcctgacgg cccgaagtga agaggtcgat
    32221 cgcctgcttg ggctggaaat tggcgctgac gactatgtgg ctaaaccgtt ttcaccccgc
    32281 gaagtgtgcg ccagggtgcg caccttactg cgtcgggtga agaagttctc gacgccgtct
    32341 cccgtcatcc gtattggaca ttttgaattg aatgaacccg cggcgcagat cagctggttt
    32401 gacacgccat taacgctgac gcggtatgag tttttattgc tgaagacgtt actcaagtcg
    32461 ccaggccgcg tctggtcccg ccagcaactg atggatagcg tgtgggaaga tgcgcaggac
    32521 acctacgatc gcaccgtcga tacccacatt aaaacgttgc gtgccaagct gcgcgccatc
    32581 aaccccgatc tttcaccgat taatactcat cgcggcatgg gatatagcct gaggggcctg
    32641 taatgcgtat cggcatgcgg ttgctgctgg gctatttttt actggtggcg gtggcagcct
    32701 ggttcgtact ggccattttt gtcaaagaag ttaaaccggg cgtgcgaaga gcaacggagg
    32761 ggacattgat cgacaccgca acgttgctgg cggagctggc gcgtcccgat ttgctctctg
    32821 gggacccaac gcatgggcaa ctggcgcagg cgtttaatca gctacagcat cgcccgtttc
    32881 gcgccaatat cggtggcatt aacaaagtgc gcaatgaata tcatgtctat atgaccgatg
    32941 cgcagggcaa agtattgttc gattcggcaa ataaagccgt tggacaggat tattcgcgct
    33001 ggaatgacgt ctggctaacg ttgcgtggtc agtatggtgc gcgcagcacg ttgcaaaatc
    33061 ctgccgatcc cgaaagttcg gtgatgtatg ttgccgcgcc gattatggac ggctcgcggc
    33121 ttattggcgt tttgagcgta ggcaaaccga acgcggcgat ggctccggtc attaaacgca
    33181 gcgagcggcg aattttatgg gccagcgcca ttttgttggg gattgcactg gtgattggcg
    33241 caggcatggt ttggtggatc aaccgctcca ttgcccggct cactcgctat gctgattccg
    33301 tcactgacaa taagcccgtt cctcttcccg atctcggtag tagcgagctg cgtaaactcg
    33361 cgcaggcgct ggaaagtatg cgcgtgaagc tggaagggaa aaactatatt gagcagtatg
    33421 tttacgcatt aactcatgag ctaaaaagcc cactggcggc gattcgtgga gcggcggaaa
    33481 ttttacgcga aggtccgccg ccggaagtgg tggctcgttt tactgacaac attctgacgc
    33541 aaaatgcgcg tatgcaggca ttggtagaaa cgttactacg ccaggcaaga ctggagaatc
    33601 gtcaggaagt cgttctgact gctgttgatg tggcggcatt attccgccgc gtcagcgaag
    33661 cgcgcaccgt gcagttggca gaaaaaaaaa tcactctgca tgttacgccc accgaggtta
    33721 acgttgctgc tgaaccggcg ttactggagc aggcgctggg aaatttactg gataacgcca
    33781 tcgattttac ccccgagagc ggttgcatca cgctaagcgc cgaagtggat caggaacacg
    33841 tcacgcttaa ggtgctggat accggtagtg gtattcctga ctacgcgctt tcacgtattt
    33901 ttgaacgctt ttactctttg cctcgtgcaa atggtcaaaa aagcagcggt ctggggttgg
    33961 cgttcgtcag tgaggtcgcc cgtttgttta acggcgaagt cacgctgcgc aacgtgcagg
    34021 aaggtggcgt gctggcttcg cttcgacttc accgtcactt cacatagctt caaattcttc
    34081 ccacatagcc ttcgtatcct gctgccattg caaaggagaa gactatgttg aaatcccccc
    34141 tgttctggaa aatgaccacc ctgtttggtg cagtattgct gttgttgatt ccgataatgc
    34201 tgattcggca ggtgattgtc gaacgtgctg attaccgtag cgatgtggaa gatgcgattc
    34261 gccaaagtac cagcgggccg caaaaactcg ttgggccgct catcgctatt cctgtgaccg
    34321 agctttatac ggtgcaggaa gaggataaaa ccgtggagcg gaaacgaagt tttatccatt
    34381 tttggttacc tgagtcattg atggttgatg gcaatcagaa cgtggaagaa cgcaagatag
    34441 ggatttatac cggtcaggtc tggcacagtg atttaacgct aaaagccgat ttcgatgttt
    34501 cgcgtcttag cgaactcaac gcgccaaata tcaccttagg caagccattt attgtgatta
    34561 gcgtcgggga tgcgcgtggt attggtgtgg tgaaagcgcc tgaagttaac ggaacggcgc
    34621 tgaccattga accgggcacc ggcttagagc aaggcgggca gggcgtgcat atccctttac
    34681 ccgaagggga ctggcgcaag cagaacctga agctgaatat ggcactgaat ttaagcggta
    34741 ccggcgatct ttctgtggtg cctgccgggc gtaatagcga aatgacctta accagcaact
    34801 ggccgcatcc cagtttttta ggtgattttc taccagccaa acgggaagtt agcgagtcag
    34861 gttttcaggc gcaatggcaa agcagctggt ttgctaataa tctcggtgag cgttttgctt
    34921 caggcaatga taccggctgg gaaaacttcc ccgcgtttag cgtcgcagtg acgacgccag
    34981 ccgatcaata ccaattaacg gaccgggcga ctaagtacgc cattctgctg attgcactga
    35041 cttttatggc gttctttgtt tttgaaacgc tcaccgcgca acgtttacac ccaatgcaat
    35101 atttgctggt ggggctttca ttggtgatgt tttatttgct cttgctggcg ctttctgaac
    35161 atatcggttt taccgtggca tggataatag ccagtctgat tggggcgtta atgaacggta
    35221 tttatttgca ggcggtattg aaaggctggc gcaatagcat gttgtttacc ctcgcgctgt
    35281 tgttgctgga tggtgtgatg tggggactgc tcaactctgc cgatagcgcg ctgttgttgg
    35341 gaaccagtgt gctggtggtg gcgctggccg gcatgatgtt tgtgacccgt aatatcgact
    35401 ggtatgcgtt ttcactgccg aaaatgaaag ccagtaaaga agttacaacg gacgatgaat
    35461 tacgtatctg gaaataaggt tgaaaaataa aaacggcgct aaaaagcgcc gttttttttg
    35521 acggtggtaa agccgattaa tcttccagat caccgcagaa gcgataacct tcaccgtgaa
    35581 tggtggcgat gatttccggc gtatccggcg tagattcgaa atgtttacga atacggcgga
    35641 tcgtcacgtc tacagtacgg tcgtgcggtt tcagctcacg gccggtcatt ttcttcagca
    35701 gttcagcacg ggactgaatt ttgcctgggt tttcacagaa gtgaagcatg gcgcggaact
    35761 cgctgcgcgg cagcttgtac tgctcgccat cagggccgat caacgaacgg ctgttgatgt
    35821 ccagttccca accattgaac ttgtagcttt caacgctacg acgttcttcg ctgacagtac
    35881 ccagattcat ggtacgggac agcaggttgc gtgcacgaat agtcagttca cgcgggttga
    35941 acggtttggt gatgtagtca tctgcaccga tttcgaggcc gagaatttta tcgacttcgt
    36001 tgtcacggcc agtcaggaac atcaacgcaa cattcgcctg ctcgcgcagt tcacgcgcta
    36061 acagaagacc gttcttaccc ggcagattga tatccatgat caccaggttg atgtcatatt
    36121 cagagaggat ctgatgcatt tccgcgccat ctgtcgcttc gaaaacatca tagccttccg
    36181 cttcgaaaat acttttcaac gtgttgcgtg ttaccaactc gtcttcaacg ataagaatgt
    36241 gcggggtctg catgtttgct acctaaattg ccaactaaat cgaaacagga agtacaaaag
    36301 tccctgacct gcctgatgca tgctgcaaat taacatgatc ggcgtaacat gactaaagta
    36361 cgtaattgcg ttcttgatgc actttccatc aacgtcaaca acatcattag cttggtcgtg
    36421 ggtactttcc ctcaggaccc gacagtgtca aaaacggctg tcatcctaac cattttaaca
    36481 gcaacataac aggctaagag gggccggaca cccaataaaa ctacgcttcg ttgacatata
    36541 tcaagttcaa ttgtagcacg ttaacagttt gatgaaatca tcgtatctaa atgctagctt
    36601 tcgtcacatt attttaataa tccaactagt tgcatcatac aactaataaa cgtggtgaat
    36661 ccaattgtcg agatttattt tttataaaat tatcctaagt aaacagaagg atatgtagca
    36721 ttttttaaca attcaaccgt tagtacagtc aggaaatagt ttagcctttt ttaagctaag
    36781 taaagggctt tttctgcgac ttacgttaag agtttgtaaa ttcgcaccgc gtaataagtt
    36841 gacagtgatc actcggttcg cggttatttg atcaagaaga gtggcaatat gcgtataacg
    36901 attattctgg tcgcacccgc cagagcagaa aatattgggg cagcggcgcg ggcaatgaaa
    36961 acgatggggt ttagcgaact gcggattgtc gatagtcagg cgcacctgga gccagccacc
    37021 cgctgggtcg cacatggatc tggtgatatt attgataata ttaaagtttt cccgacactg
    37081 gctgaatcgt tacacgatgt cgatttcact gtcgccacca ctgcgcgcag tcgggcgaaa
    37141 tatcattact acgctacgcc agtagaactg gtgccgctgt tagaggaaaa atcttcatgg
    37201 atgagccatg ccgcgctggt gtttggtcgc gaagattccg ggttgactaa cgaagagtta
    37261 gcgttggctg acgttcttac tggtgtgccg atggtggcgg attatccttc gctcaatctg
    37321 gggcaggcgg tgatggtcta ttgctatcaa ttagcaacat taatacaaca accggcgaaa
    37381 agtgacacaa cggcagacaa acatcaactg caagctttac gcgaacgagc catggcactg
    37441 ctgacgactc tggcagtggc agatgacata aaactggtcg actggttaca acaacgcctg
    37501 gggcttttag agcaacgaga cacggcaatg ttgcaccgtt tgctgcatga tattgaaaaa
    37561 aatatcacca aataaaaaac gccttagtaa gtatttttca gcttttcatt ctgactgcaa
    37621 cgggcaatat gtctctgtgt ggattaaaaa aagagtgtct gatagcagct tctgaactgg
    37681 ttacctgccg tgagtaaatt aaaattttat tgacttaggt cactaaatac tttaaccaat
    37741 ataggcatag cgcacagaca gataaaaatt acagagtaca caacatccat gaaacgcatt
    37801 agcaccacca ttaccaccac catcaccatt accacaggta acggtgcggg ctgacgcgta
    37861 caggaaacac agaaaaaagc ccgcacctga cagtgcgggc ttttttttcg accaaaggta
    37921 acgaggtaac aaccatgcga gtgttgaagt tcggcggtac atcagtggca aatgcagaac
    37981 gttttctgcg tgttgccgat attctggaaa gcaatgccag gcaggggcag gtggccaccg
    38041 tcctctctgc ccccgccaaa atcaccaacc acctggtggc gatgattgaa aaaaccatta
    38101 gcggccagga tgctttaccc aatatcagcg atgccgaacg tatttttgcc gaacttttga
    38161 cgggactcgc cgccgcccag ccggggttcc cgctggcgca attgaaaact ttcgtcgatc
    38221 aggaatttgc ccaaataaaa catgtcctgc atggcattag tttgttgggg cagtgcccgg
    38281 atagcatcaa cgctgcgctg atttgccgtg gcgagaaaat gtcgatcgcc attatggccg
    38341 gcgtgttaga agcgcgtggt cacaacgtta ccgttatcga tccggtcgaa aaactactgg
    38401 cagtggggca ttacctcgaa tctaccgtcg atattgctga gtccacccgc cgtattgcgg
    38461 caagtcgtat tccggctgat cacatggtgc tgatggcagg cttcaccgcc ggtaatgaaa
    38521 aaggcgaact ggtggtactt ggacgcaacg gttccgacta ctccgcggcg gtgctggctg
    38581 cctgtttacg cgccgattgt tgcgagattt ggacggacgt tgacggggtc tatacctgcg
    38641 acccgcgtca ggtgcccgat gcgaggttgt tgaagtcgat gtcctaccag gaagcgatgg
    38701 agctttccta cttcggcgct aaagttcttc acccccgcac cattaccccc atcgcccagt
    38761 tccagatccc ttgcctgatt aaaaataccg gaaatcctca agcaccaggt acgctcattg
    38821 gtgccagtcg tgatgaagac gaattaccgg tcaagggcat ttccaatctg aataacatgg
    38881 caatgttcag cgtttccggc ccggggatga aaggaatggt cggcatggcg gcgcgcgtct
    38941 ttgccgcgat gtcacgcgcc cgtatttccg tggtgctgat tacgcaatca tcttccgaat
    39001 acagtatcag tttctgcgtt ccgcaaagcg actgtgtgcg ggctgaacgg gcaatgcagg
    39061 aagagttcta cctggaactg aaagaaggct tactggagcc gctggcggtg acggaacggc
    39121 tggccattat ctcggtggta ggtgatggta tgcgcacctt gcgtgggatc tcggcgaaat
    39181 tctttgccgc gctggcccgc gccaatatca acattgtcgc cattgctcag ggatcttctg
    39241 aacgctcaat ctctgtcgtg gtaaataacg atgatgcgac cactggcgtg cgcgttactc
    39301 atcagatgct gttcaatacc gatcaggtta tcgaagtgtt tgtgattggc gtcggtggcg
    39361 ttggcggtgc gctgctggag caactgaagc gtcaacaaag ctggctgaag aataaacata
    39421 tcgacttacg tgtctgcggt gttgccaact cgaaggcact gctcaccaat gtgcatggcc
    39481 taaatctgga aaactggcag gaagaactgg cgcaagccaa agagccgttt aatctcgggc
    39541 gcttaattcg cctcgtgaaa gaatatcatc tgctgaaccc ggtcattgtt gactgcacct
    39601 ccagccaggc agtggcggat caatatgccg acttcttgcg cgaaggtttc cacgttgtca
    39661 cgccgaacaa aaaggccaac acctcgtcga tggattacta ccatctgttg cgtcatgcgg
    39721 cggaaaaatc gcggcgtaaa ttcctctatg acaccaacgt tggggctgga ttaccggtta
    39781 ttgagaacct gcaaaatctg ctcaatgctg gtgatgaatt gatgaagttc tccggcattc
    39841 tttcaggttc gctttcttat atcttcggca agttagacga aggcatgagt ttctccgagg
    39901 cgactactct ggcgcgggaa atgggttata ccgaaccgga cccgcgagat gatctttctg
    39961 gtatggatgt ggcgcgtaaa ctattgattc tcgctcgtga aacgggacgt gaactggagc
    40021 tggcggatat tgaaattgaa cctgtgctgc ccgctgagtt taacgccgag ggtgatgttg
    40081 ccgcttttat ggcgaatctg tcacagctcg atgatctctt tgccgcgcgc gtggcgaagg
    40141 cccgtgatga aggaaaagtt ttgcgctatg ttggcaatat tgatgaagat ggcgtctgcc
    40201 gcgtgaagat tgccgaagtg gatggtaatg atccgctgtt caaagtgaaa aatggcgaaa
    40261 acgccctggc cttttatagc cactattatc agccgctgcc gttggtgctg cgcggatatg
    40321 gtgcgggcaa tgacgttaca gctgccggtg tctttgccga tctgctacgt accctctcat
    40381 ggaagttagg agtctgacat ggttaaagtt tatgcccctg cttccagtgc caatatgagc
    40441 gtcgggtttg atgtgctcgg ggcggcggtg acacctgttg atggtgcatt gctcggagat
    40501 gtagtcacgg ttgaggcggc agagacattc agtctcaaca acctcggacg ctttgccgat
    40561 aagctgccgt cagaacctcg ggaaaatatc gtttatcagt gttgggagcg tttttgccag
    40621 gagctgggta agcaaattcc agtggcgatg accctggaaa agaatatgcc gatcggttcg
    40681 ggcttaggct ccagcgcctg ttcagtggtc gcggcgctga tggcgatgaa tgaacactgc
    40741 ggcaagccgc ttaatgacac tcgtttgctg gctttgatgg gcgagctgga aggccgtatc
    40801 tccggcagca ttcattacga caacgtggca ccgtgttttc ttggtggtat gcagttgatg
    40861 atcgaagaaa acgacatcat cagccagcaa gtgccagggt ttgatgagtg gctgtgggtg
    40921 ctggcgtatc cggggattaa agtctcgacg gcagaagccc gggctatttt accggcgcag
    40981 tatcgccgcc aggattgcat tgcgcacggg cgacatctgg caggctttat tcacgcctgc
    41041 tattcccgtc agcctgagct tgccgcgaag ctgatgaaag atgttatcgc tgaaccctac
    41101 cgtgaacggt tactgccagg cttccggcag gcgcggcagg cggtcgcgga aatcggcgcg
    41161 gtagcgagcg gtatctccgg ctccggcccg actttgttcg ctctgtgtga caagccggat
    41221 accgcccagc gcgttgccga ctggttgggt aagaactacc tgcaaaatca ggaaggtttt
    41281 gttcatattt gccggctgga tacggcgggc gcacgagtac tggaaaacta aatgaaactc
    41341 tacaatctta aagatcacaa tgagcaggtc agctttgcgc aagccgtaac ccaggggttg
    41401 ggcaaaaatc aggggctgtt tttcccgcac gacctgccgg aattcagcct gactgaaatt
    41461 gatgagatgc tgaagctgga ttttgtcacc cgcagtgcga agatcctctc ggcgtttatt
    41521 ggtgatgaaa tcccgcagga aatcctggaa gagcgcgtac gcgcggcgtt tgccttcccg
    41581 gctccggtcg ccaatgttga aagcgatgtc ggttgtctgg agttgtttca cgggccaacg
    41641 cttgcattta aagatttcgg cggtcgcttt atggcacaaa tgctgaccca tattgcgggc
    41701 gataagccag tgaccattct gaccgcgacc tccggtgata ccggagcggc agtggctcat
    41761 gctttctacg gtttgccgaa tgtgaaagtg gttatcctct atccacgagg caaaatcagt
    41821 ccactgcaag aaaaactgtt ctgtacattg ggcggcaata ttgaaactgt tgccatcgac
    41881 ggcgatttcg atgcctgtca ggcgctggtg aagcaggcgt ttgatgatga agagctgaaa
    41941 gtggcgctgg ggttaaactc ggctaactcg attaacatca gccgtttgct ggcgcagatt
    42001 tgctactact ttgaagctgt tgcgcagctg ccgcaggaag cgcgcaacca gctggttgtc
    42061 tcggtgccaa gcggaaactt cggcgatttg acggcgggtc tgctggcgaa gtcactcggt
    42121 ctgccggtga aacgttttat tgctgcgacc aacgtgaacg ataccgtgcc acgtttcctg
    42181 cacgacggtc agtggtcccc caaagcgact caggcgacgt tatccaacgc gatggacgtg
    42241 agtcagccga acaactggcc gcgtgtggaa gagttgttcc gccgcaaaat ctggcaactg
    42301 aaagagctgg gttatgcagc cgtggatgat gaaaccacgc aacagacaat gcgtgagtta
    42361 aaagaactgg gctacacctc ggagccgcac gctgccgtag cttatcgtgc gctgcgtgac
    42421 cagttgaatc caggcgaata tggcttgttc ctcggcaccg cgcatccggc gaaatttaaa
    42481 gagagcgtgg aagcgattct cggtgaaacg ttggatctgc caaaagagct ggcagaacgt
    42541 gctgatttac ccttgctttc gcataatctg cccgccgatt ttgctgcgtt gcgtaaattg
    42601 atgatgaatc atcagtaata tctattcatt atctcaatca ggccgggttt gcttttatgc
    42661 agcccggctt ttttgtgaag aaaatatgga gagaaacgac agggaaaaag gagaaattct
    42721 caataaatgc ggtaacttag agattaggat tgcggagaat aacaaccgcc gttctcatcg
    42781 cgtaatctcc ggatatcgac ccataacggg caatgataaa aggagtaacc tgtgaaaaag
    42841 atgcaatcta tcgtactcgc actttccctg gttctggtcg ctcccatggc agcgcaggct
    42901 gcggaaatta cgttagtccc gtcagtaaaa ttacagatag gcgatcgtga taatcgtggc
    42961 tattactggg atggcggtca ctggcgcgac cacggctggt ggaaacaaca ttatgaatgg
    43021 cgaggcaatc gctggcaccc atatggaccg ccgccatcgc cgcgccataa caagcacaat
    43081 gatcatcgtg gcgatcatcg tccggggcct gacaaacatc atcgctaaat atcaatgccg
    43141 gattgccatc cggcattgac tacaaactta acgctgctcg tagcgtttaa acaccagttc
    43201 accattgctg gaggaagctt catcaaagaa gtaaccttcg ctattaaaac cagtcagttg
    43261 ctctggtttg gtcagccgat tttcaataat gaaacgactc atcagaccgc gtgctttctt
    43321 agcgtagaag ctgatgatct taaatttgcc gttcttctca tcgaggaaca ccggcttgat
    43381 aatctcggca ttcaatttct tcggcttcac cgatttaaaa tactcatctg acgccagatt
    43441 aatcaccaca ttatcgcctt gtgctgcgag cgcctcgttc agcttgttgg tgatgatatc
    43501 tccccagaat tgatacagat ctttccctcg ggcattctca agacggatcc ccatttccag
    43561 acgataaggc tgcattaaat cgagcgggcg cagtacgcca tacaagccgg aaagcattcg
    43621 caaatgctgt tgggcaaaat cgaaatcgtc ttcgctgaag gtttcggcct gcaagccggt
    43681 gtagacatca cctttaaatg ccagaatcgc ctggcgggca ttctccggcg tgaaatctgg
    43741 ctgccagtca tgaaagcgag cggcgttgat acccgccagt ttgtcgctga tgcgcatcag
    43801 cgtgctaatc tgcggaggcg tcagtttccg cgcttcatgg atcaactgct gggaattgtc
    43861 taacagctcc ggcagcgtat agcgcgtggt ggtcaacggg ctttggtaat caagcgtttt
    43921 cgcaggtgaa ataagaatca gcatatccag tccttgcagg aaatttatgc cgactttagc
    43981 aaaaaaagag aatgagttga tcgatagttg tgattactcc tgcgaaacat catcccacgt
    44041 gtccggagaa agctggcgac cgatatccgg ataacgcaac ggatcaaaca ccgggcgcac
    44101 gccgagttta cgctggcgta gataatcact ggcaatggta tgaaccacag gcgagagcag
    44161 taatatggcg gtcaaattgg taatagccat gcaggccatt atgatatctg ccagttgcca
    44221 catcagcgga agacttagca aggtgccgcc gatgaccgtt gcgaaggtgc agattcgcag
    44281 acaccagatc gctttagggt tgttcaggcg taagaagaag agattgtttt cggcataaat
    44341 gtagttggca acgatggagc tgaaggcaaa cagaataacc acgagggtaa caaactccgc
    44401 accccaggaa cccattaaca cccgcatcgc cttctggata agctgaatac cttccagtgg
    44461 catgtaggtt gtgccgttac ccgccagtaa tatcagcatg gcgcttgccg tacagatgac
    44521 cagggtgtcg ataaaaatgc caatcatctg gacaattcct tgcgctgcgg gatgcggagg
    44581 ccaggacgca gccgccgctg ccgcgtttgg cgtcgacccc attcccgcct cattggaaaa
    44641 catactgcgc tgaaaaccgt tagtaatcgc ctggcttaag gtatatcccg ccgcgccgcc
    44701 tgccgcttcc tgccagccaa aagcactctc aaaaatagac caaatgacgt ggggaagttg
    44761 cccgatattc attacgcaaa ttaccaggct ggtcagtacc cagattaacg ccatcaacgg
    44821 gacaaacccc tgcatgagcc gggcgacgcc atgaagaccg cgagtgattg ccagcagagc
    44881 aaagacagca agaataatgc ctgtcaccag cggaggaaaa tcaaaagaaa aacttagggc
    44941 gcgggcaacg gcgttcgcct gaactccgct gaaaattatg ccgtaggcga tgagcaaaaa
    45001 gacggcgaac agaacgccca tccaacgcat ccccagcccg cgcgccatat accatgccgg
    45061 tccgccacga aactgcccat tgacgtcacg ttctttataa agttgtgcaa gggaacattc
    45121 ggcaaacgag gtcgccatgc cgataaacgc ggcaacccac atccagaaga cggctccagg
    45181 tccaccggcg gtaatagcca gcgcaacgcc ggccaggttg ccgctaccca cgcgtgccgc
    45241 aagactggta cacaatgact gaaatgaggt taaaccgcct ggctgtggat gaatgctatt
    45301 tttaagactt ttgccaaact ggcggatgta gcgaaactgc acaaatccgg tgcgaaaagt
    45361 gaaccaacaa cctgcgccga agagcaggta aatcattacc gatccccaaa ggacgctgtt
    45421 aataaaggag aaaaaatctg gcatgcatat ccctcttatt gccggtcgcg atgactttcc
    45481 tgtgtaaacg ttaccaattg tttaagaagt atatacgcta cgaggtactt gataacttct
    45541 gcgtagcata catgaggttt tgtataaaaa tggcgggcga tatcaacgca gtgtcagaaa
    45601 tccgaaacag tctcgcctgg cgataaccgt cttgtcggcg gttgcgctga cgttgcgtcg
    45661 tgatatcatc agggcagacc ggttacatcc ccctaacaag ctgtttaaag agaaatacta
    45721 tcatgacgga caaattgacc tcccttcgtc agtacaccac cgtagtggcc gacactgggg
    45781 acatcgcggc aatgaagctg tatcaaccgc aggatgccac aaccaaccct tctctcattc
    45841 ttaacgcagc gcagattccg gaataccgta agttgattga tgatgctgtc gcctgggcga
    45901 aacagcagag caacgatcgc gcgcagcaga tcgtggacgc gaccgacaaa ctggcagtaa
    45961 atattggtct ggaaatcctg aaactggttc cgggccgtat ctcaactgaa gttgatgcgc
    46021 gtctttccta tgacaccgaa gcgtcaattg cgaaagcaaa acgcctgatc aaactctaca
    46081 acgatgcagg gattagcaac gatcgtattc tgatcaaact ggcttctacc tggcagggta
    46141 tccgtgctgc agaacagctg gaaaaagaag gtattaactg taacctgaca ctgctgttct
    46201 ccttcgctca ggctcgtgct tgtgcggaag cgggcgtgtt cctgatctcg ccgtttgttg
    46261 gccgtattct tgactggtac aaagcgaata ccgacaagaa agagtacgct ccggcagaag
    46321 atccgggcgt ggtttctgta tctgaaatct accagtacta caaagagcac ggttatgaaa
    46381 ccgtggtgat gggcgcaagc ttccgtaaca tcggcgaaat tctggaactg gcaggctgcg
    46441 accgtctgac catcgcaccg gcactgctga aagagctggc tgagagcgaa ggggctatcg
    46501 aacgtaaact gtcttacacc ggcgaagtga aagcgcgtcc ggcgcgtatc actgagtccg
    46561 agttcctgtg gcagcataac caggatccaa tggcagtaga taaactggcg gaaggtatcc
    46621 gtaagtttgc tgttgaccag gaaaaactgg aaaaaatgat cggcgatctg ctgtaatcat
    46681 tcttagcgtg accgggaagt tggtcacgct acctcttctg aagcctgtct gtcactccct
    46741 tcgcagtgta tcattctgtt taacgagact gtttaaacgg aaaaatcttg atgaatactt
    46801 tacgtattgg cttagtttcc atctctgatc gcgcatccag cggcgtttat caggataaag
    46861 gcatccctgc gttggaagaa tggctgacat cggcgctaac cacgccgttt gaactggaaa
    46921 cccgcttaat ccccgacgag caggcgatca tcgagcaaac gttgtgtgag ctggtggatg
    46981 aaatgagttg ccatctggtg ctcaccacgg gcggaactgg cccggcgcgt cgtgacgtaa
    47041 cgcccgatgc gacgctggca gtagcggacc gcgagatgcc aggctttggt gaacagatgc
    47101 gccagatcag cctgcatttt gtaccaactg cgatcctttc gcgtcaggtg ggtgtgattc
    47161 gcaaacaggc gctgatcctt aacttacccg gtcagccgaa gtcgattaaa gagacgctgg
    47221 aaggtgtaaa ggacgctgag ggtaacgttg tggtgcacgg tatttttgcc agcgtaccgt
    47281 actgcattca gttgctggaa gggccatacg ttgaaacggc accggaagtg gttgctgcat
    47341 tcagaccgaa gagtgcaaga cgcgaagtta gcgaataaaa aaatcccccc gagcgggggg
    47401 atctcaaaac aattagtgcg attcaccaat cggcagaacg gtgcgaccaa actgctcgtt
    47461 cagcacttca cccatcgcca gatagattgc gctggcacca cagatcagac caatccagcc
    47521 ggcaaagtgg atgatcgcgg cgttaccggc aatgttaccg atcgccagca gggcaaacag
    47581 cacggtcagg ctaaagaaaa cgaattgcag aacgcgtgcg cctttcagcg tgccgaagaa
    47641 cataaacagc gtaaatacgc cccacagacc caggtagaca ccaaggaact gtgcatttgg
    47701 cgcatcggtc agacccagtt tcggcatcag cagaatcgca accagcgtca gccagaaaga
    47761 accgtaagag gtgaatgcgg ttaaaccgaa agtgttgcct tttttgtact ccagcagacc
    47821 agcaaaaatt tgcgcgatgc cgccgtagaa aatgcccatg gcaagaataa taccgtccag
    47881 agcgaaataa cccacgttgt gcaggttaag cagaatggtg gtcatgccga agcccatcag
    47941 gcccagcggt gccggattag ccaacttagt gttgcccata attcctcaaa aatcatcatc
    48001 gaatgaatgg tgaaataatt tccctgaata actgtagtgt tttcagggcg cggcataata
    48061 atcagccagt ggggcagtgt ctacgatctt ttgaggggaa aatgaaaatt ttccccggtt
    48121 tccggtatca gacctgagtg gcactaacca tccggcgcag gcaggcgatt tgcagtacgg
    48181 ctggaatcgt cacgcgatag gcgctgccgc tgaccgcttt aaccccattt agtgccgcgc
    48241 ctacagggcc tcccagcccc gcgccgcgca gcaaaccatg cccaagtacg ctcattgctg
    48301 cgtgggtgcg taaaatgcgg gtcaattggc tggaaagcaa atgcgacacg ccttttgcca
    48361 ataatttgtc tttcatcagc agcggcagca gctcttccag ctcattcacc ctggcatcga
    48421 ctgcgtgcag aaactcctgc ttatgttcct cgtccatttt cttccaggta ttacgcagaa
    48481 attgttccag taactgttgc tcaatttcaa acgtagacat ctctttgtcg gctttcagct
    48541 tcaatcgctt tgaaacatcg agcaaaatgg cccgatacaa tttaccgtgt ccacgcagtt
    48601 tgttggcgat actatcgcca ccaaaatgtt gtaattctcc ggcaatcagc tgccagttgc
    48661 ggcgatgttg ctcgggatgt ccttccatcg atttaaacag ttcgttgcgc atcagtacgc
    48721 tggagaggcg agttttgcct ttttcattat gggtgagcag ccgggcgaaa tttgccaatt
    48781 gttcctcact acaatgctgg agaaaatcca gatctgaatc attcaggtaa ttaacattca
    48841 ttttttgtgg cttctatatt ctggcgttag tcgtcgccga taattttcag cgtggccata
    48901 tccgatgagt taaccgtatg accggaaaag gtgatttttg agacgcagcg tttattgtcg
    48961 ttatcgctgt taatgttgat ccagtcagtg gtttgccctt cttttatttc tgaaggaata
    49021 ttcagactct gactggcgct acgggcgact ttgaaataaa ccgatgcacc gcttaactgc
    49081 aaatcgccat ggtcggctga gagttgtatg cgtttaacaa ttcggcaaac aggaagtttc
    49141 agcgccagat cgttggtttc gttacgtggc attgcaatga cgccgaggat tttatggtcg
    49201 tttgcctgcg ccgtgcagca cagcatcagg ctaatcgcca ggctggcgga aatcgtaaaa
    49261 acggatttca taaggattct cttagtgggg agaggtaggg gatgaatacc cactagttta
    49321 ctgctgataa agagaagatt caggtacgta atattttctt tttattacaa tttttctatg
    49381 aatgtcttgg ctgtcattca ttctctatat gaataaaatt gctgtcagtt tcgtgtcttg
    49441 ttctggcata tcgcgaaatt tctgcgcaaa agcacaaaaa atttttgcat ctcccccttg
    49501 atgacgtggg ttacgacccc atttagtagt caaccgcagt gagtgagtct gcaaaaaaat
    49561 gaaattgggc agttgaaacc agacgtttcg cccctattac agactcacaa ccacatgatg
    49621 accgaatata tagtggagac gtttagatgg gtaaaataat tggtatcgac ctgggtacta
    49681 ccaactcttg tgtagcgatt atggatggca ccactcctcg tgtactggag aacgccgaag
    49741 gcgatcgcac cacgccttct atcattgcct atacccagga tggtgaaact ctggttggtc
    49801 agccggctaa acgtcaggca gtgacgaacc cgcaaaacac cctgtttgcg attaaacgcc
    49861 tgattggccg ccgcttccag gacgaagaag tacagcgtga tgtttccatc atgccgttca
    49921 aaattattgc tgctgataac ggcgacgcat gggtcgaagt taaaggccag aaaatggcac
    49981 cgccgcagat ttctgctgaa gtgctgaaaa aaatgaagaa aaccgctgaa gattacctgg
    50041 gtgaaccggt aactgaagct gttatcaccg taccggcata ctttaacgat gctcagcgtc
    50101 aggcaaccaa agacgcaggc cgtatcgctg gtctggaagt aaaacgtatc atcaacgaac
    50161 cgaccgcagc tgcgctggct tacggtctgg acaaaggtac tggcaaccgt actatcgcgg
    50221 tttatgacct gggtggtggt actttcgata tttctattat cgaaatcgac gaagttgacg
    50281 gcgaaaaaac cttcgaagtt ctggctacca acggtgatac ccacctgggt ggtgaagact
    50341 tcgacagccg tctgatcaac tacctggttg aagaattcaa gaaagatcag ggcattgacc
    50401 tgcgcaacga tccgctggca atgcagcgcc tgaaagaagc ggcagaaaaa gcgaaaatcg
    50461 aactgtcttc cgctcagcag accgacgtta acctgccgta catcactgca gacgcgaccg
    50521 gtccgaaaca catgaacatc aaagtgactc gtgcgaaact ggaaagcctg gttgaagatc
    50581 tggtaaaccg ttccattgag ccgctgaaag ttgcactgca ggacgctggc ctgtccgtat
    50641 ctgatatcga cgacgttatc ctcgttggtg gtcagactcg tatgccaatg gttcagaaga
    50701 aagttgctga gttctttggt aaagagccgc gtaaagacgt taacccggac gaagctgtag
    50761 caatcggtgc tgctgttcag ggtggtgttc tgactggtga cgtaaaagac gtactgctgc
    50821 tggacgttac cccgctgtct ctgggtatcg aaaccatggg cggtgtgatg acgacgctga
    50881 tcgcgaaaaa caccactatc ccgaccaagc acagccaggt gttctctacc gctgaagaca
    50941 accagtctgc ggtaaccatc catgtgctgc agggtgaacg taaacgtgca gctgataaca
    51001 aatctctggg tcagttcaac ctggatggta tcaacccggc accgcgcggc atgccgcaga
    51061 tcgaagttac cttcgatatc gatgctgacg gtatcctgca cgtttccgcg aaagataaaa
    51121 acagcggtaa agagcagaag atcaccatca aggcttcttc tggtctgaac gaagatgaaa
    51181 tccagaaaat ggtacgcgac gcagaagcta acgccgaagc tgaccgtaag tttgaagagc
    51241 tggtacagac tcgcaaccag ggcgaccatc tgctgcacag cacccgtaag caggttgaag
    51301 aagcgggcga caaactgccg gctgacgaca aaactgctat cgagtctgca ctgactgcac
    51361 tggaaactgc tctgaaaggt gaagacaaag ccgctatcga agcgaaaatg caggaactgg
    51421 cacaggtttc ccagaaactg atggaaatcg cccagcagca acatgcccag cagcagactg
    51481 ccggtgctga tgcttctgca aacaacgcga aagatgacga tgttgtcgac gctgaatttg
    51541 aagaagtcaa agacaaaaaa taatcgccct ataaacgggt aattatactg acacgggcga
    51601 aggggaattt cctctccgcc cgtgcattca tctaggggca atttaaaaaa gatggctaag
    51661 caagattatt acgagatttt aggcgtttcc aaaacagcgg aagagcgtga aatcaaaaag
    51721 gcctacaaac gcctggccat gaaataccac ccggaccgta accagggtga caaagaggcc
    51781 gaggcgaaat ttaaagagat caaggaagct tatgaagttc tgaccgactc gcaaaaacgt
    51841 gcggcatacg atcagtatgg tcatgctgcg tttgagcaag gtggcatggg cggcggcggt
    51901 ttcggcggcg gcgcagactt cagcgatatt tttggtgacg ttttcggcga tatttttggc
    51961 ggcggacgtg gtcgtcaacg tgcggcgcgc ggtgctgatt tacgctataa catggagctc
    52021 accctcgaag aagctgtacg tggcgtgacc aaagagatcc gcattccgac tctggaagag
    52081 tgtgacgttt gccacggtag cggtgcaaaa ccaggtacac agccgcagac ctgtccgacc
    52141 tgtcatggtt ctggtcaggt gcagatgcgc cagggtttct ttgccgtgca gcagacctgt
    52201 ccacactgtc agggccgcgg tacgctgatc aaagatccgt gcaacaaatg tcatggtcat
    52261 ggtcgtgttg agcgcagcaa aacgctgtcc gttaaaatcc cggcaggagt ggacactgga
    52321 gaccgcatcc gtcttgcggg tgaaggtgaa gcgggcgagc atggcgcacc ggcaggcgat
    52381 ctgtacgttc aggttcaggt taaacagcac ccgattttcg agcgtgaagg taacaacctg
    52441 tattgcgaag tcccgatcaa cttcgctatg gcagcgctgg gtggcgaaat cgaagtaccg
    52501 acccttgatg gtcgcgtcaa actgaaagtg cctggcgaaa cccagaccgg taagctgttc
    52561 cgtatgcgcg gtaaaggcgt caagtctgtt cgcggcggtg cgcaaggtga cttgctgtgc
    52621 cgcgttgtcg tcgaaacacc ggtaggtctg aacgagaagc agaaacagct gctgcaagag
    52681 ctgcaggaaa gcttcggtgg cccaaccggt gagcacaaca gcccgcgttc aaagagcttc
    52741 tttgatggtg tgaagaagtt ttttgacgac ctgactcgct aacctcccca aaagcctgcc
    52801 tgtgggcagg ccagggtaaa aatagggtgc gttgaagata tgcgagcacc tgtaaagtgg
    52861 cggggatcac tccccgccgt tgctcttact cggattcgta agccgtgaaa acagcaacct
    52921 ccgtctggcc agttcggatg tgaacctcac agaggtcttt tctcgttacc agcgccgcca
    52981 ctacggcggt gatacagatg acgatcaggg cgacaatcat cgccttatgc tgcttcattg
    53041 ctctcttctc cttgacctta cggtcagtaa gaggcactct acatgtgttc agcatatagg
    53101 gagcctcggg ttgatggtaa aatatcactc ggggcttttc tctatctgcc gttcagctaa
    53161 tgcctgagac agacagcctc aagcacccgc cgctattata tcgctctctt taaccaattc
    53221 tgttttatcg attctaatcc tgaagacgcc tcgcattttt atggcgtaat tttttaatga
    53281 tttaattatt taactttaat ttatctcttc atcgcaatta ttgacgacaa actggattat
    53341 ttttgaagta ttggcctaac aaacatcgcc gactgacaac aaattaatta ttacttttcc
    53401 taattaatcc ctcagaaatc ctgaccttaa gctatgatta tcagggttta gggtcactcg
    53461 tgagcgctta cagccgtcaa aaactcatct catcgctgat ggcacaaatc cttcaatggt
    53521 tcgtaaaaaa cgaattattc ttacactata atctgatttt aacgatgatt cgtgcggggt
    53581 aagatagtaa aaacgatcta ttcacctgaa agagaaataa aaagtgaaac atctgcatcg
    53641 attctttagc agtgatgcct cgggaggcat tattcttatc attgccgcta tcctggcgat
    53701 gattatggcc aacagcggcg caaccagtgg atggtatcac gactttctgg agacgccggt
    53761 tcagctccgg gttggttcac tcgaaatcaa caaaaacatg ctgttatgga taaatgacgc
    53821 gctgatggcg gtatttttcc tgttagtcgg tctggaagtt aaacgtgaac tgatgcaagg
    53881 atcgctagcc agcttacgcc aggctgcatt tccagttatc gccgctattg gtgggatgat
    53941 tgtgccggca ttgctctatc tggcctttaa ctatgccgat ccgattaccc gcgaagggtg
    54001 ggcgatcccg gcggctactg acattgcttt tgcacttggt gtgctggcgc tgttgggaag
    54061 tcgtgttccg ttagcactga agatcttttt gatggctctg gctattatcg acgatcttgg
    54121 ggccatcatt atcatcgcat tgttctacac taatgactta tcgatggcct ctcttggtgt
    54181 cgcggctgta gcaattgcgg tactcgcggt attgaatctg tgtggtgtac gccgcacggg
    54241 cgtttatatt ctggttggcg tggtgttgtg gactgcggtg ttgaaatcgg gggttcacgc
    54301 aaccctggcg ggggtaattg tcggcttctt tattcctttg aaagagaagc atgggcgttc
    54361 tccagcgaag cgactggagc atgtgttgca cccgtgggtg gcgtatctga ttttaccgct
    54421 gtttgcattt gctaatgctg gcgtttcact gcaaggcgtc acgctggatg gcttgacctc
    54481 cattctacca ttggggatca tcgctggctt gctgattggc aaaccgctgg ggattagtct
    54541 gttctgctgg ttggcgctgc gtttgaaact ggcgcatctg cctgagggaa cgacttatca
    54601 gcaaattatg gcggtgggga tcctgtgcgg tatcggtttt actatgtcta tctttattgc
    54661 cagcctggcc tttggtagcg tagatccaga actgattaac tgggcgaaac tcggtatcct
    54721 ggtcggttct atctcttcgg cggtaattgg atacagctgg ttacgcgttc gtttgcgtcc
    54781 atcagtttga caggacggtt taccggggag ccataaacgg ctcccttttc attgttatca
    54841 gggagagaaa tgagcatgtc tcatatcaat tacaaccact tgtattactt ctggcatgtc
    54901 tataaagaag gttccgtggt tggcgcagcg gaggcgcttt atttaacacc acaaaccatt
    54961 accggacaga ttcgggcgct ggaagagcgc ctgcaaggca aattatttaa acgcaaggga
    55021 cgtggtctcg aacccagcga gctgggagag ctggtctatc gctatgccga taaaatgttc
    55081 accttaagcc aggaaatgct ggatatcgtg aactatcgca aagaatccaa tttattgttt
    55141 gacgttggcg tggctgatgc actttctaaa cgcctggtca gtagcgtact taacgccgca
    55201 gtggtagaag gcgagccgat tcatcttcgc tgcttcgaat ccacccacga aatgctgctg
    55261 gagcaattaa gtcagcataa actggatatg atcatttccg actgtccgat agattctacg
    55321 cagcaggaag gtctgttctc cgtgagaatt ggcgaatgtg gcgtgagttt ctggtgtaca
    55381 aatccaccac cggaaaaacc gttcccggct tgtctggaag aacggcggct tttgattcct
    55441 gggcgacgtt caatgttagg gcgcaaattg cttaactggt ttaactccca gggattaaac
    55501 gtagaaatcc tcggcgagtt tgatgatgcc gcgttgatga aagcttttgg tgcgatgcac
    55561 aatgcaatct tcgttgcccc aacgctttat gcatatgact tttatgccga taaaactgtc
    55621 gtagaaattg gtcgcgtcga aaatgtgatg gaagagtacc atgctatttt tgctgagcgg
    55681 atgattcagc acccggcggt acagcgaatc tgcaatacgg attattctgc gctttttagc
    55741 ccagcggcac gttaatcggc agctccccca aagttaaggt gggggagata gattagttgt
    55801 acattaccac gattttgact cggctcatta tttgcccgct tgagacatta tttccatatg
    55861 tacgcgggcg aataaataga ggaatctgat tacttccttc atggggatgc tgaaaagagt
    55921 agtaattgct tttaccatac tgcatttcat gcgtaccatc attggagagt tgaaagccta
    55981 tttgacccgc accgtcctca ctgttttcta aatcattttt taatatgctg gttcctgaat
    56041 cctgtatcgt cactggctca aagtaatatt caacatcact aggttcaaaa actaattcac
    56101 cttgctcacc ctggatacag tcaaatctta tatgatgaaa ctgataattt tcattattga
    56161 tacgagacag tacaaatcgt ccagtgtcga tggattgatc ttcaaaacca caggtcatta
    56221 aatttacctg taaaatagta tttaaattaa aattaaagcg gacgctatta caattagtat
    56281 tattccctgt acatgaactc acttcaattt cgaacaacaa aggaaaagga gttcctggaa
    56341 aaatgtctgc ggtgttagaa ctatctgata cagatgcgat agaatatgga atggtgaaat
    56401 ttatcgcaga tagcatttct tctaaattat aagataactt ccctggaagc tctatttttt
    56461 taccgtcaga ttcaaagggg aaattaataa tgtaatgacg acttccgact ttaaacctta
    56521 aagcgttctg tgttaaaaaa tgaactttaa tggcgttggt cgtctcttgc aagtctgcac
    56581 actcaatgat attcccggtg ttttcaattt tttcggtggt tagattacct ttcatactca
    56641 gtggcttgag tatatatgtt ccacgttgtt caccgacatt ggtcactgca cagggagcac
    56701 tttgcacaat aaaagaatat agactcaagg tgattaataa aagccacttc atagtaaact
    56761 ccttttactg gcatatatat gttttgtttt catcgataac gtgttgaaaa gtaatagtac
    56821 agaataagtt ctgttcatca ttgagagcaa cttttatgcc agtgggaatt tcatctacgc
    56881 gaataaaaag gcgactaccc tggccaacga ctccaatatt gttttcctgg agatccagta
    56941 cgtcatagcc aaaggttaat ggcgaaccat cgggacgcag tgcctgtata taccagggct
    57001 tacgttggtc agttgtaaac tgcacataag aaactgctcc acggtaaggc gcaataattt
    57061 gacgattgcc ctgcaattct gtttcacttt cactgtttgc tatatcaagc accagatggt
    57121 tttcacgatg aggtataatc aggtcgtaaa caacctggcc tttgctgttt gttcggttgt
    57181 atttttgtcc attaatatgc gcgccttcta acccaggcgc atccagaatt gcaaatgtat
    57241 cggataactg gttagttata ttaataccac cgggccagac aactaatcca ctactaatac
    57301 ttccaccact ttgccaggcg tttttagaat ggctatagct gccatctatt gtggcgatgg
    57361 gagttctcca gcttaaattc gtaccaagcg aggtatcatt attttgttgt tgctgattaa
    57421 catatatacc ataatttaac tgatcatgtt caccggctat gccagtaatt ccagtattgt
    57481 tggaggaata gccatctttg gaaaatgagg tcgaattcga taagttaatt tggtgacgtg
    57541 ttttggcaat atcatccccc cagtagaaag gaatggagat aaacagatta aaacgctcct
    57601 cttctttgtc gttttcatca taggattggc tggcagagaa agtataactg atgcgttgcc
    57661 agctattgga ataactgaat tggtaatctt tagcatttcc acttcgcccc cagtaattcc
    57721 gccaaagcgc acttaatgat acatttccca gattattgga taaaggttgc atgatattgg
    57781 cagaaaggct attttttctt ccaatatcat aaaaatcgtc atagtcactc tgatgattta
    57841 ttttatcatt ttcgtaaaga tggtcgctga atgtcctgta atcctgcgaa gcataacgcc
    57901 aggcggccac gctaaagtga gtagccgttt gcaatagata tttattatag gcgacctgat
    57961 agctcgtacc ttcgtgtctg gtatcattat ttaacttact actcgatcgg gtagcatcaa
    58021 aagagattgc tcccagcggt gtattccaac catttcccag agttatagca ttatagttat
    58081 cggataaaat cgtaccgcca taaagtgtca aaagattgtt gaggccatat atataatttg
    58141 cctcgagaaa gtcctcctga ttcttgacac catatatctg actgcgtccg gcggtaaaat
    58201 cgaaatttga aacaccaggt tgcagcatat tgggcacgga agagtagggc accaaaaagg
    58261 aacgaacact accatctgct tccttaatac tgacatcgag atcagagccg ctgcccgaaa
    58321 gttgcaaatc tgcgatagta aatggtcctg ggggaacctc tttttggtaa atggtatagc
    58381 cattttgcga aaccgttatc aaggcgttac tttgcgcaac accttgtact aaaggtgtaa
    58441 agctttgcat agaatcaggt agcatttgca tatcgcgaaa taagcgtatt ccgctaaatc
    58501 gcagagagtc gaatatgagt gaagatgtgt aattttctcc tatctgaact gtgcttaata
    58561 tttgcgacca gccacgttcc aggtaaagag tattactttg ccatttgcct ttcatatcat
    58621 caggtttgct ataactggca tcagagtgta actgccaact aaataaattt aatccgctgt
    58681 taaaccggcc ataactggct gtattactgt tattattgct gtcataagag cgatactgac
    58741 ttaaattata ggaggtataa aaagcatcaa tgccgcgatc ccaactttct gggggaacgt
    58801 agcctctttc aagttcattg atatacgcct gtggtactgt taaatttagt cggtgttcac
    58861 tgatatccca ctgatattgt ccaccatgaa ccgcttcatc cagtaaaata caattatctt
    58921 ctgtattgag gttatcggtc tttaccccca gtgtggttag cagcacctta ggcagacatg
    58981 gtttggctgg tgactctggg atcgtgatat cttttttccc acgccactgg tgatttacat
    59041 aaaaatcaag ttcgtagttt cctggaagcg gttgtttatt atcgaaacga tattcggaaa
    59101 ctttttctcc tctcatgccg ccaatcatga agtgcgtatc aaaagtttct tcagcataac
    59161 ccggaaaaga atacaaaaaa ataatagcag cgacccatcg tgtcgtcctt aacatgactt
    59221 tttcctgtta tctcgcggcg attttactat ggatgttatt cccgtaatca tcaactaccg
    59281 ttagttcata ttctgatgcg tggttatttt ttaatgtaat gtcgttattc gaaaatggag
    59341 gcagtaagat actttcatta ttaactttca cgttttgtgc tttgatagtt gtaaccgtta
    59401 tccagttggc tgtttcgttg tttattgaga tggcgttatt tttctttttg atatttaatt
    59461 gagaaaggct ttttttatct accggagcga ttccgcttgg acgccaaagt aactttatac
    59521 ggttttgcaa tgcaagttta attttgtttt ttcctgcatt ttgtgggttg tttggaggga
    59581 tatccagaac atttagataa aataaacttt ctttattgtg aggcaaattg tttggcattt
    59641 ttcttatttt aagttgttgg ccagaaccac ctgctacacg acttactggc ggtgtgagaa
    59701 taaatgggac ctgaattttt tctggcgcga tagtggtgtt cccatcatcg atccatgcct
    59761 gaatcaaaga ggcattttta ctttgattca ttaactgtac gagaacttct ctgttttttt
    59821 ccggataaat aatgcgcgta ccatagatga ccacaccggc atgaacaggt aaaataaaaa
    59881 atgacatcag taatgctgtt attattctca cgtaatcact ccgtaaataa taccggtagc
    59941 agagctaccg gcaaaaaacg ttaatctgca ctgattttaa tcgttacggt tgactctacg
    60001 gagccttttg taacaggatt gccacttgtg cgggcatatt gtgcacttaa attaagaaaa
    60061 tattgaccag agccattatc tttcagcggt gtttttaacc ccggtctcat tctggtcaaa
    60121 gttccatcat cgaggaaaat ggcaaagcca atgttctcag cagttccttt attcagcgta
    60181 aagttattag catcaaactc tgatgctagc acttcgatac ttggggctgt attgccgtca
    60241 cactgaattt ctaagtcatg agaggttgaa ccggttttta aagtatagat ttcattattg
    60301 ataagttgat tcattgcact ggttacaaca tcttcgacaa cgatatctgt tgtcgaatca
    60361 ccattaacaa agtaacacgt tgtatcaaca acttctcctc taatggttag cgttccttga
    60421 tcgacatcct tggcgaaggt tgttgcgcta aacatcaacg ttgtcgcaac gaagggaata
    60481 atatactttt tcataaatca tccttaatta actggtatgg aaggtcac
//
LOCUS       DACBVI010000011        58858 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.011, whole genome shotgun sequence.
ACCESSION   DACBVI010000011 DACBVI010000000
VERSION     DACBVI010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.011"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            132..1196
                     /gene="ulaG"
                     /locus_tag="IU325_03495"
     CDS             132..1196
                     /gene="ulaG"
                     /locus_tag="IU325_03495"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP5808105.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1304..2059
                     /gene="ulaR"
                     /locus_tag="IU325_03500"
     CDS             1304..2059
                     /gene="ulaR"
                     /locus_tag="IU325_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HAP5808106.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2056..2805)
                     /locus_tag="IU325_03505"
     CDS             complement(2056..2805)
                     /locus_tag="IU325_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP5808107.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCAIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFRGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLADRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            2987..3316
                     /gene="bsmA"
                     /locus_tag="IU325_03510"
     CDS             2987..3316
                     /gene="bsmA"
                     /locus_tag="IU325_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HAP5808108.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3465..3740
                     /gene="yjfN"
                     /locus_tag="IU325_03515"
     CDS             3465..3740
                     /gene="yjfN"
                     /locus_tag="IU325_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HAP5808109.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3857..5482)
                     /locus_tag="IU325_03520"
     CDS             complement(3857..5482)
                     /locus_tag="IU325_03520"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HAP5808110.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIEQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGIPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFMEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLNTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(5566..6729)
                     /locus_tag="IU325_03525"
     CDS             complement(5566..6729)
                     /locus_tag="IU325_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAP5808111.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGVIPRDTDQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKLQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6732..7370)
                     /locus_tag="IU325_03530"
     CDS             complement(6732..7370)
                     /locus_tag="IU325_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HAP5808112.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNSDICAHGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEQFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(7380..7778)
                     /locus_tag="IU325_03535"
     CDS             complement(7380..7778)
                     /locus_tag="IU325_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HAP5808113.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(7796..8455)
                     /locus_tag="IU325_03540"
     CDS             complement(7796..8455)
                     /locus_tag="IU325_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2491 family protein"
                     /protein_id="HAP5808114.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSRIDLGGGSQIFRYYTSGDEFLQINTTGGENIDDIDDIKLFVYE
                     ESYGISKESHWRETINAKAMGAMTLNWQEKRWLRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(8506..9204)
                     /locus_tag="IU325_03545"
     CDS             complement(8506..9204)
                     /locus_tag="IU325_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HAP5808115.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALKALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(9223..9624)
                     /locus_tag="IU325_03550"
     CDS             complement(9223..9624)
                     /locus_tag="IU325_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HAP5808116.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQHEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSL"
     gene            complement(9751..10482)
                     /gene="rlmB"
                     /locus_tag="IU325_03555"
     CDS             complement(9751..10482)
                     /gene="rlmB"
                     /locus_tag="IU325_03555"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP5808117.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(10573..13014)
                     /locus_tag="IU325_03560"
     CDS             complement(10573..13014)
                     /locus_tag="IU325_03560"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP5808118.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKPKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(13053..13478)
                     /gene="nsrR"
                     /locus_tag="IU325_03565"
     CDS             complement(13053..13478)
                     /gene="nsrR"
                     /locus_tag="IU325_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HAP5808119.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(13683..14981)
                     /gene="purA"
                     /locus_tag="IU325_03570"
     CDS             complement(13683..14981)
                     /gene="purA"
                     /locus_tag="IU325_03570"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP5808120.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(15085..15282)
                     /locus_tag="IU325_03575"
     CDS             complement(15085..15282)
                     /locus_tag="IU325_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 family protein"
                     /protein_id="HAP5808121.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(15364..16368)
                     /gene="hflC"
                     /locus_tag="IU325_03580"
     CDS             complement(15364..16368)
                     /gene="hflC"
                     /locus_tag="IU325_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HAP5808122.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(16371..17630)
                     /gene="hflK"
                     /locus_tag="IU325_03585"
     CDS             complement(16371..17630)
                     /gene="hflK"
                     /locus_tag="IU325_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HAP5808123.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(17716..18996)
                     /gene="hflX"
                     /locus_tag="IU325_03590"
     CDS             complement(17716..18996)
                     /gene="hflX"
                     /locus_tag="IU325_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP5808124.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(19073..19381)
                     /gene="hfq"
                     /locus_tag="IU325_03595"
     CDS             complement(19073..19381)
                     /gene="hfq"
                     /locus_tag="IU325_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HAP5808125.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(19467..20417)
                     /gene="miaA"
                     /locus_tag="IU325_03600"
     CDS             complement(19467..20417)
                     /gene="miaA"
                     /locus_tag="IU325_03600"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP5808126.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(20410..22257)
                     /gene="mutL"
                     /locus_tag="IU325_03605"
     CDS             complement(20410..22257)
                     /gene="mutL"
                     /locus_tag="IU325_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP5808127.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAVREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RGGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(22267..23604)
                     /gene="amiB"
                     /locus_tag="IU325_03610"
     CDS             complement(22267..23604)
                     /gene="amiB"
                     /locus_tag="IU325_03610"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HAP5808128.1"
                     /translation="MMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGTAAQTASTVTTPDRTLPN"
     gene            complement(23623..24084)
                     /gene="tsaE"
                     /locus_tag="IU325_03615"
     CDS             complement(23623..24084)
                     /gene="tsaE"
                     /locus_tag="IU325_03615"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP5808129.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(24056..25603)
                     /locus_tag="IU325_03620"
     CDS             complement(24056..25603)
                     /locus_tag="IU325_03620"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HAP5808130.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRTAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALSREAWLNAGGEIHASNTVWPESVDLIVDALLGTGLQQAPRESISQLIDHANTH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRVVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVLVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            25602..26741
                     /gene="queG"
                     /locus_tag="IU325_03625"
     CDS             25602..26741
                     /gene="queG"
                     /locus_tag="IU325_03625"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP5808131.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSASEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(26724..26777)
                     /locus_tag="IU325_03630"
     CDS             complement(26724..26777)
                     /locus_tag="IU325_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808132.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(27011..27086)
                     /locus_tag="IU325_03635"
     tRNA            complement(27011..27086)
                     /locus_tag="IU325_03635"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27051..27053),aa:Gly,seq:gcc)
     gene            complement(27122..27197)
                     /locus_tag="IU325_03640"
     tRNA            complement(27122..27197)
                     /locus_tag="IU325_03640"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27162..27164),aa:Gly,seq:gcc)
     gene            complement(27355..27430)
                     /locus_tag="IU325_03645"
     tRNA            complement(27355..27430)
                     /locus_tag="IU325_03645"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27395..27397),aa:Gly,seq:gcc)
     gene            complement(27641..28186)
                     /locus_tag="IU325_03650"
     CDS             complement(27641..28186)
                     /locus_tag="IU325_03650"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HAP5808133.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            28281..29333
                     /gene="rsgA"
                     /locus_tag="IU325_03655"
     CDS             28281..29333
                     /gene="rsgA"
                     /locus_tag="IU325_03655"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HAP5808134.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLLIEPIIVLN
                     KIDLLDDEGMEFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNTLLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            29430..30398
                     /gene="psd"
                     /locus_tag="IU325_03660"
     CDS             29430..30398
                     /gene="psd"
                     /locus_tag="IU325_03660"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HAP5808135.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLSAEEIEAEHDASPLVDDKKDQV"
     gene            30420..33743
                     /gene="mscM"
                     /locus_tag="IU325_03665"
     CDS             30420..33743
                     /gene="mscM"
                     /locus_tag="IU325_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HAP5808136.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNSPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPQLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVLIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(33772..34086)
                     /locus_tag="IU325_03670"
     CDS             complement(33772..34086)
                     /locus_tag="IU325_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HAP5808137.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEDDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(34184..35686)
                     /gene="yjeM"
                     /locus_tag="IU325_03675"
     CDS             complement(34184..35686)
                     /gene="yjeM"
                     /locus_tag="IU325_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HAP5808138.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRITGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWVVE"
     gene            complement(35905..36882)
                     /gene="epmA"
                     /locus_tag="IU325_03680"
     CDS             complement(35905..36882)
                     /gene="epmA"
                     /locus_tag="IU325_03680"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAP5808139.1"
                     /translation="MSETASWQPSASIPNLLKRATIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            37207..39015
                     /gene="frdA"
                     /locus_tag="IU325_03685"
     CDS             37207..39015
                     /gene="frdA"
                     /locus_tag="IU325_03685"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HAP5808140.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            39008..39742
                     /locus_tag="IU325_03690"
     CDS             39008..39742
                     /locus_tag="IU325_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAP5808141.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            39753..40148
                     /gene="frdC"
                     /locus_tag="IU325_03695"
     CDS             39753..40148
                     /gene="frdC"
                     /locus_tag="IU325_03695"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAP5808142.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPESWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            40159..40518
                     /gene="frdD"
                     /locus_tag="IU325_03700"
     CDS             40159..40518
                     /gene="frdD"
                     /locus_tag="IU325_03700"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAP5808143.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            40581..41714
                     /gene="blaEC"
                     /locus_tag="IU325_03705"
     CDS             40581..41714
                     /gene="blaEC"
                     /locus_tag="IU325_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAP5808144.1"
                     /translation="MFKMTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLTHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDITEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKAITPPAPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            41802..42335
                     /gene="blc"
                     /locus_tag="IU325_03710"
     CDS             41802..42335
                     /gene="blc"
                     /locus_tag="IU325_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Blc"
                     /protein_id="HAP5808145.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDTKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGDPRRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAIAT
                     REGFDISKFIWVQQPGS"
     gene            complement(42332..42649)
                     /locus_tag="IU325_03715"
     CDS             complement(42332..42649)
                     /locus_tag="IU325_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HAP5808146.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(42824..42970)
                     /gene="ecnB"
                     /locus_tag="IU325_03720"
     CDS             complement(42824..42970)
                     /gene="ecnB"
                     /locus_tag="IU325_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAP5808147.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(43081..43206)
                     /gene="ecnA"
                     /locus_tag="IU325_03725"
     CDS             complement(43081..43206)
                     /gene="ecnA"
                     /locus_tag="IU325_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAP5808148.1"
                     /translation="MMKRLIVLVLLASALLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(43258..43824)
                     /gene="efp"
                     /locus_tag="IU325_03730"
     CDS             complement(43258..43824)
                     /gene="efp"
                     /locus_tag="IU325_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP5808149.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            43866..44894
                     /gene="epmB"
                     /locus_tag="IU325_03735"
     CDS             43866..44894
                     /gene="epmB"
                     /locus_tag="IU325_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAP5808150.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            45129..45998
                     /locus_tag="IU325_03740"
     CDS             45129..45998
                     /locus_tag="IU325_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808151.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFLSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVINELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(46048..46401)
                     /locus_tag="IU325_03745"
     CDS             complement(46048..46401)
                     /locus_tag="IU325_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HAP5808152.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(46539..48185)
                     /gene="groL"
                     /locus_tag="IU325_03750"
     CDS             complement(46539..48185)
                     /gene="groL"
                     /locus_tag="IU325_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP5808153.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(48229..48522)
                     /gene="groES"
                     /locus_tag="IU325_03755"
     CDS             complement(48229..48522)
                     /gene="groES"
                     /locus_tag="IU325_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP5808154.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            48798..50054
                     /gene="yjeH"
                     /locus_tag="IU325_03760"
     CDS             48798..50054
                     /gene="yjeH"
                     /locus_tag="IU325_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HAP5808155.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALGINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(50070..50546)
                     /gene="fxsA"
                     /locus_tag="IU325_03765"
     CDS             complement(50070..50546)
                     /gene="fxsA"
                     /locus_tag="IU325_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HAP5808156.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            50883..52319
                     /gene="aspA"
                     /locus_tag="IU325_03770"
     CDS             50883..52319
                     /gene="aspA"
                     /locus_tag="IU325_03770"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HAP5808157.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            52437..53738
                     /locus_tag="IU325_03775"
     CDS             52437..53738
                     /locus_tag="IU325_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAP5808158.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            53853..54191
                     /gene="cutA"
                     /locus_tag="IU325_03780"
     CDS             53853..54191
                     /gene="cutA"
                     /locus_tag="IU325_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HAP5808159.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            54167..55864
                     /gene="dsbD"
                     /locus_tag="IU325_03785"
     CDS             54167..55864
                     /gene="dsbD"
                     /locus_tag="IU325_03785"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HAP5808160.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVLADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQVRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMLLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            55901..56476
                     /locus_tag="IU325_03790"
     CDS             55901..56476
                     /locus_tag="IU325_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP5808161.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            56583..56658
                     /locus_tag="IU325_03795"
     tRNA            56583..56658
                     /locus_tag="IU325_03795"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56616..56618,aa:Phe,seq:gaa)
     gene            57275..58813
                     /locus_tag="IU325_03800"
     CDS             57275..58813
                     /locus_tag="IU325_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadC family transcriptional regulator"
                     /protein_id="HAP5808162.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSV
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQEMLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
BASE COUNT        14389 a        15740 c        14573 g        14156 t
ORIGIN      
        1 tcaaaatcaa caacaacttc cctggcaaaa agtcaaaaaa tggtcattgc cttcctgaaa
       61 aaatagtgtc aggattaatc aaaaccaatc acatattgat tcgaattgaa ctatcatgaa
      121 ggtaaatggc gatgagtaaa gtgaaaagta tcacccgtga atcctggatc ctgagcactt
      181 tcccggagtg gggtagctgg ctgaatgaag aaattgaaca agagcaggtc gcccctggca
      241 catttgcgat gtggtggcta ggctgcaccg ggatctggct gaaatcagaa ggtggcacca
      301 acgtttgcgt tgatttctgg tgcggcactg gcaaacaaag tcacggtaac ccgttaatga
      361 aacagggtca ccagatgcag cgcatggctg gcgtgaaaaa actgcagcca aacctgcgca
      421 ccaccccgtt tgttcttgat ccgtttgcga ttcgccagat cgacgcggta ctggcgactc
      481 acgatcacaa cgatcatatc gacgttaacg tcgctgctgc cgtgatgcag aactgtgctg
      541 atgacgtacc gttcattgga ccgaaaacct gcgttgattt gtggattggc tggggcgtac
      601 cgaaagagcg ttgcatcgtg gtcaaacctg gcgatgttgt aaaagtgaaa gacattgaaa
      661 ttcatgcgct tgacgctttc gaccgtactg cactgatcac cctgcctgcc gatcaaaaag
      721 cggctggcgt actgccagat ggcatggacg atcgcgcagt aaactacctg ttcaaaaccc
      781 ctggcggttc cctgtatcac agtggcgatt cccactactc taactattat gcgaagcacg
      841 gtaacgaaca tcagatcgat gtggcgttag gatcgtatgg tgagaacccg cgcggtatta
      901 ccgacaaaat gaccagcgcc gatatgctgc gtatgggtga agccctgaat gcgaaagtgg
      961 ttatcccgtt ccaccacgac atctggtcaa acttccaggc cgatccgcaa gaaatccgcg
     1021 tgctgtggga gatgaaaaaa gatcgtctga agtatggctt caagccgttt atctggcagg
     1081 tgggcggcaa atttacctgg ccgttggata aagacaactt cgagtaccac tatccgcgtg
     1141 gtttcgatga ttgcttcact attgaaccgg atctgccgtt caagtcattc ctgtaatctg
     1201 tcgtttatac cggatagcgt ggagtagttc gcgctatccg gcgtagtatt tcctggctat
     1261 ttcaaatatc atctaaaaaa atcaaatttt atcggaatag ctcatgactg aagcacaaag
     1321 acatcaaatc ctcctggaaa tgctcgcaca attgggcttt gtgaccgttg agaaagtcgt
     1381 tgagcgtctg ggaatttcgc ctgccactgc gcgacgcgat atcaataaac ttgacgaaag
     1441 cggcaaactg aaaaaagtgc gcaatggcgc agaagctatt acccagcagc gcccacgctg
     1501 gacgccgatg aatctgcatc aggcgcagaa tcacgatgaa aaagtacgta ttgctaaagc
     1561 ggcctcgcag ctggttaatc cgggcgaaag cgtagtcatc aactgcggct ccaccgcgtt
     1621 tctgcttggg cgggaaatgt gtggcaagcc tgtgcaaatc atcactaatt atctaccgct
     1681 ggcaaattac ctgatcgatc aggagcatga cagcgtgatc attatgggcg gacaatacaa
     1741 caaaagtcag tccatcactt taagcccgca aggcagcgaa aacagtctct atgccgggca
     1801 ctggatgttt accagcggca aagggctgac agcagaaggg ttgtataaaa ccgatatgct
     1861 gacggcaatg gcagagcaga agatgctaag cgtggtaggg aaactggtgg tactggttga
     1921 tagcagtaag atcggcgaac gcgcgggaat gctttttagc cgtgccgatc aaatcgatat
     1981 gctgatcacc ggcaaaaatg ctaacccgga aatcctgcaa caactggaag cgcaaggggt
     2041 cagcattctg cgtgtttaaa gatgctggcg gaaaaatgtc acggcagcat ctaacgcctc
     2101 aggcgtaatg cggtgacgca cgcctggctg ccatgaacag gttagcagtt tatcccgtcc
     2161 cgtctcgctt aaggcctgct gcaaacgtag tgattcgtcg gcaggcacga cgtcatcgtc
     2221 aaggccatgc cacagcagta gaggtctgtc accaagttgt tccaggtggt ttgtcgcttc
     2281 ccactctgcc agcggcgcga caatgttatt gaattcattc tgctgtgctg ccgtttcagg
     2341 tatcagcggt ggaaacagtg aacgggcgag cgatgtaaaa tagcccgatc ccatcatgct
     2401 ggcggtacat ttcaccgtgg ggtggcgagc ggtaattccc agtgccgtca tcgcgcccat
     2461 cgacgcgcca ccgactgcca gacggtcagc aagcagccag ttttcttcgg ctattgccgc
     2521 acgtaacgta gtgaattcct gcatactttg tagcaaaatt tgccaaaatt gatttaaccg
     2581 ccgcgctgcg tcgcccctaa aacggctacc gtgatcgggc gcatccggca tgatcacccg
     2641 caaaccagcc tgcgccagcg caacggcaaa atagctatac accagactgg acgaagtaaa
     2701 gccgtgataa aaaatcgcgc acggtaacgg ggtatctttt tgcccgacag gataagcatg
     2761 aagaacggga atatctgcca gctcgcgtga ttctatttca atcatctgtc tctccttttt
     2821 gtctggtatg acatgaagaa atccttagca caagtttctg ctggcggtat tagttcataa
     2881 tgttgagatg tgggttacgc tttcgtcacg ttttcattcg aaaatcgcgt accaggtaac
     2941 aattcgggaa cattccccca aagttaaatt aataaggcac tacactatgg ttagcaggaa
     3001 acgtaatagc gttatttacc ggtttgccag tttattattg gtgttaatgt taagtgcctg
     3061 tagcgcactg caaggtacgc cacagccagc accgccagta acggaccatc cgcaagagat
     3121 tcgccgcgac cagacgcaag ggttacagcg aataggtagc gtaagcacga tggttcgggg
     3181 ttctccggat gacgcattag cagaaatcag agcgaaagct gtcgctgcaa aagctgatta
     3241 ttacgttgtc gtcatggtgg acgaaaccat cgtgacagga cagtggtatt cacaagccat
     3301 tttgtaccgt aaataaatca gacaacttac atagtcagct ttacattgct ttgcgtccct
     3361 atgcgtttac ctgtgcacaa taaattaccg gctggaagcc tgggcgttta ttaccgcgat
     3421 ggaatgccct gcaacatgtg gggaaacgaa aaatggagct gacgatgaaa caattacttg
     3481 cctcaccctc gctgcaatta gtgacttatc ctgcgagcgc cacggcacag tctgcagaat
     3541 tcgccagtgc tgactgcgtg acgggcctga atgaaatagg tcagatctca gttagcaata
     3601 tttcagggga tccgcaggac gtggaacgta tcgtggcgtt aaaggccgat gaacaaggtg
     3661 cgtcatggta ccgcattatt accatgtacg aagaccaaca gcctgataac tggcgtgtac
     3721 aggcgatact gtatgcgtga gtgtcatctg aagcgaatga acaatgccgg atgcggcgcg
     3781 agcgccttgt ccggcctaca attttcacat atttcattta gttaatcgaa accagcgtcg
     3841 catccgtcgt atacgcttac aaacacactc cccccgtcgc ccgcagcaat aaatcattct
     3901 ggatctgctc tgacaaccgc acgccaccgc gcgtatttaa catcacctga caccacgcct
     3961 gtgccattgg cggagaagca tatttcaaca tttgcgcacc gcagcccagc aggaatagct
     4021 gatgagtaat ctctcgcccc agttcttcag ctggcttacg caactgctgc tgtaaacgac
     4081 gaaccgcgcg atcaaaataa cgatcttgcc ctttcacttc cataaacgct tccgacagca
     4141 agtcgtatac tcccgcttgc ttattaagaa cgcgcagcac atccaggcac ataatattgc
     4201 cggaaccttc ccaaatactg tttaccggca tctcccggta aagccgcggc agctcgctct
     4261 cttcgcaata gccaataccg cccagcacct ccatcgcctc agccacaaac ggaatgccac
     4321 gtttgcagat cacaaatttc gccgcaggcg taaataagcg cgcccacagg gcttctttgg
     4381 catcggcacg ccgatcccac gctcgcgcaa gacgaaacag caacgccgtt tgcccttcaa
     4441 gctgaagcgc catgcgactt aagacatggc gcataagggg ctgttctatt aacggattac
     4501 caaatacatg gcgttgatga gcgtgatata tcgccagcga gaacgcacgg cgcatcatgg
     4561 catggctacc cagggcgcaa tcaaaacgcg tcatcccacc cattttcagg atcagacgaa
     4621 tcccttcccc ttcctgaccc aacaaccagc caatggcatc ctgaaactcc acttcgcaac
     4681 tggcattgga acgattaccc agcttgtctt tcagccgctc gaggcgaatc gcgttgcgtt
     4741 gcccgtcagg caaaaagcgc ggcacaaaaa agcaggacag accgcccgcg gtctgcgcca
     4801 gcaccagatg cgcatcgctt tgtggcactg agaaaaacca tttatgcccc accagccgat
     4861 aagagccatc ttccagacgc tctgcgcggg tggtgttgct catcacatcg gaaccgccct
     4921 gcttttccgt cattcccatg ccaatcaaca aaccgcgttt ttgcccacct ggcaataagt
     4981 gagaatcgta gcgatcgctc aacagcggcg tggtccagtc ctgaaacggc gtgggtaaca
     5041 tctgtaacag caatggcgtg gcggcaaagg tcatggttat cggacataac gacccagcct
     5101 caacctgcgc atgtaacata aaacgcgccg cgcgcgccac aaatgcgccg gagcgagcgt
     5161 cttcttccca ggccaggttg tgtacccgat tggtacataa cgcctgcatc agcaggtgcc
     5221 atgcgggatg gaaacggaca tcgtccagac gccgcccctg ggcatcgtag cgcaatagtt
     5281 caggtgggtt tacattcgcc agtcgcccca gttcaaggga ttcagccgtt ccgagttgct
     5341 gaccaatact ggcgagaaaa tcgctatccc agccagcgcc ttcgcgtgtt accgcttcgc
     5401 agagcgcgcc atcagataag tataaattgc tgttatttaa gggtataggt tgattaaaaa
     5461 cagtgtgagt ttgccagtgc actgtgtctc cctccatcaa tggcagtcac cattagtatg
     5521 gtcactgcca cggattcccg acagaaagag attgctaaaa cattcttaac ccgcaatgta
     5581 gtgcggaaca aaacgcgaag tgtctttagt gatcagcgtg ttatcttcgc ggatacccat
     5641 accgcacgct tcatcatcga caatccagct accaatcagc gtgtaactat cgccaaatcg
     5701 cggtagaggt tgaaacgcct gatagatcat cggttcatcg gcgtaatcac cgtcagcgtg
     5761 gtcaacgacg ttattctgac cgtcgaaaat ggtgacgtta ccgccctcgc gcgagtagat
     5821 cggtttacgc acatagcttt cgccagcggc gatctgcagt ttttcgccct caaaccagga
     5881 cgccagaaga ttaggatgac caggaaagaa gcgccaaagc agcggcatta gccctttgtt
     5941 actcaagata cttttccata acggctccac ccattgctcg cggcgcttgc gcagcagcgg
     6001 accgttatcg tcacgcatca tccactccag cgggtacagc ttaaatgcac gctggatgac
     6061 attatcatca agatcggtca gtacaccgcc gacgcccaaa ccgagatctt caatgtagat
     6121 aaaccgcgac tcctgccctg cctgctgggc gcagtcctgc aagtacagca cggtactcct
     6181 gtcttcgtca gtgtcctgac aacagcaaaa ataaaacggc tcccgactat aaagctcgct
     6241 aaagcgcgaa atcaggcgtt cctgaatggc attgtactga tcggtatcac gcggaataac
     6301 gccgctgcgc cgggcatcct ccagccacag ccactgaaaa taagccgatt catacaacga
     6361 ggttggcgta tcggcgttgt actccagcag cttcaccggc gcattgccac accaggcaaa
     6421 atccatccgg ccatacagcg aaggatcgcg tgcgcgccag ctttcagcga tcacatccca
     6481 gtacaacgga gggatcgcca gttgcgtcag gatctcttcg tctttaaccg cgcgatccac
     6541 cacctcaagg cacatctgat gcagttccgc agtcggtttt tcgatctgct cttcaatctg
     6601 gcgcagagta aaacggtaag cccgactctc gtcccaatag atttcattgt cgatgatatg
     6661 aaagtcgaaa ccgttgtcag cggcgatctg gtccagatcc cgccgcacag gaacgttgtg
     6721 tctcagcatg attagcctcc ccaatgccca cgggcgctgg aagaacgacc atagccgccg
     6781 cgagaaacgg ttgacgcttt tttggtggta aagccgcccg aagagtaaga ctcttttttg
     6841 ccagaaccgg agcgccagga gtaatcacca gaagtgttgc gccagacggg gcgcgaagca
     6901 aaagaggaac caccgctgtt atacacaaac tgctcatcgc gatctttacg aataacccgg
     6961 cttaacaaaa atccagaaac gaccgggatc caactctgtt caacgttgtc gtagtaacaa
     7021 ttttcatact tagactgaca attctgctga gtcatattct tcggaacatc ggcataaaac
     7081 gccgctttgg cgttgttcca gccatgagca cagatatctg aattattacc gtcatcaata
     7141 caatcctgca cagtcgagta aaacgttccg tcgccgtcgt tatcgacatc gccactatcg
     7201 ccacaacctt tcaatacgaa aaaagcggca ccgcccatta gcgccaacgt taagtatttt
     7261 tgtgcgtagc gattgcgacg cggttcaaaa ggattattcg gtctgccaat gcgactgatc
     7321 gctccgtgac caattttact gttatttctt gatttgcgtt ttctggccat gatcgtccat
     7381 taccatgtca tacaagctgc gttaaaaata ccaccagcca gtgcggcggt tcccataaac
     7441 attcctgctg cggtattgtg attaataatt ttttcgctta atgcgggcat ataaagcctg
     7501 acgccagcaa aaacgagtaa ttgaatcacc agagcaatcc cgccccaggc gaaatagtct
     7561 ggaatactca ccgcattgat tgccgcactg gataagggga tcacgtaacc caacaatgta
     7621 ccgctgaacg ccagtgacgc cgcggtatta ttgtttttga ttaactgcca ttcgttgtgc
     7681 ggtgtaattt tagaatagat aaacagaaaa ataatcacca tagccacgcc aataaaaaaa
     7741 taggcactaa aggcaagaag agaatccagt atatgcattg taaacgtcct tgtaattaac
     7801 caataatatg aagtgatgtc agcgggatat cgacacccaa cgcgcgcgaa aataaccact
     7861 ctggctcgcc gagatcgtta aaagattcct caccatttag cagcaaatat tcgtaagtat
     7921 cttcggttac ttgccgttgg tagcccatgg taaaattatg tacttcccat ttggcatggt
     7981 tttgattttc tactttttcc agcatgtaga cgggttcgat atttcccggt tcttcgctgt
     8041 taaaaaatcg cagccagcgt ttttcctgcc agtttaaggt cattgccccc atcgctttgg
     8101 cattgatcgt ctcgcgccag tgactctctt tactgatacc gtagctctct tcatagacaa
     8161 agagcttgat atcatcaatg tcatcaatat tttcgccgcc ggtggtattg atttgcagaa
     8221 actcatcgcc tgaagtgtag tagcggaaaa tctggctacc gccgcccaga tcgatgcggc
     8281 tgacggcggc taccgtaaat tcctcacccg gcagcgcaat cagcaattca tcttccagca
     8341 aacgaaacgc taacgtgtcg agcgtaaaac cactattgag atgcagaccc agcggaccgc
     8401 gagcgatagc aggcttatta tccttgccaa acagacgctg gaaaaaacca gacatatttt
     8461 atccttaaaa tagttgccag ccttgcagtg aggctggcaa aaaaattact cgccctgttg
     8521 gcgttgcagt cttgctaata catcctgggc gctactttta ttgctaccgc ctattccggc
     8581 ttccgccagc ttttcatcaa gatcgcgacc gtctgcgact ttttccaact gtgcagcagc
     8641 atccagacga gcctgacgtt cggcctgacg cgtttgcagg cgttttaagg attctgccgc
     8701 tgtcgaaacg ctggaagatg cgccaacggt agaggtcgtt acagcctgtt gtgcacgctg
     8761 catggcttca gtggctttaa cgacttccat ttgctgctca aactgagcga tacgctgcgc
     8821 tgtcgctgta actgcttttt ccacgccatc acgagagact tccagattcg acaacacttg
     8881 ctcttcagcg gtaatgagat tctcaaggcg cgcgatttct tcagcaactt cgttaatcaa
     8941 cgacggatta acgttcttgc ttaacgcttt cagcgcacga gcttccagac tggctttgcg
     9001 ctcacgtaaa tctttcagct tatcgtgact taatttcacc cgcgccagca gatcaacgcg
     9061 agattttccg gctttatcga gttcagcttt agcgtcacgg atatgctgtt ccagcatgcg
     9121 cacgccctgg gtttcttcaa tggattcttc cgcctgggag ataaacgatt tccccagcgt
     9181 aaataaactt tttaaaattc ccataaatac ccgtccttgt aattagagtg aatattcttc
     9241 tgtaatttca gccagatcca atgcgttatc taccagcgag gttatctcaa gcaggatatc
     9301 ttcaagagaa gatttaagcg ataacgcccc gaaaacaatg taatactctt catgttgcac
     9361 gctggagatc cctaccgatg acaacggcat catcttctga tttcttaata agaaggtatt
     9421 aaattcatct ggattgctga tactactcac cggacaaata aaggtttcga taatcatttg
     9481 gcgggaagta aaaagaatat tgatttgcaa atcgccataa tcattcattt taataattaa
     9541 tgcgttctcg ctatttgtta catcaatatt ttgttcaggt acagtttgca gcgccgtcgc
     9601 cagcgccaac ggattccatg tcatatatta ttccatatag atttagttta aatattaaca
     9661 aaatgaatat ttgcagtacg taattatctt accagctaaa cacaaaaaaa aaccatccaa
     9721 atctggatgg cttttcataa ttcagaggaa ctagctgcgc tgtcgcaccg cttcaaataa
     9781 gcaaatccct gtcgcaaccg aaacgttcag ggaagaaacg cttcctgcca ttgggatgct
     9841 gatcaactcg tcgcaatgtt cacgagtcag gcgacgcata ccttcacctt ccgcgcccat
     9901 caccagtgcc agccgtccgg tcattttgct ctgataaagt gtatgatccg cctcacctgc
     9961 cgtaccgacg atccagatat tctcttcctg caacatacgc atggtgcgcg ccagattagt
    10021 cacccgaatc agcggaacgc tttctgccgc accacaggcc acttttttcg ccgtggcgtt
    10081 gagctgtgcg gagcgatctt tcggcacaat caccgcatga acaccagcgg cgtccgcgct
    10141 acgcaggcac gcgccgaggt tgtgcggatc ggttacgccg tcgaggatca gcaggaacgg
    10201 ttgatcgagc gaagcgatca gatccggcag atcgttttcc tgatactgcc gtcctggctt
    10261 cacacgggcg ataatgccct gatgcacggc accgtcgctt ttctcgtcga gatactgacg
    10321 gttcgccaac tggataacca cgccctggga ctcaagggcg tgaatcagcg gtaacagacg
    10381 tttatcttca cggcctttta aaataaagac ttcctgaaaa cgttcagggg cgcgctccag
    10441 cagggcctgc actgcgtgga tgccgtaaat catttcgctc attaatgtac tcgttgttta
    10501 cgttttgccg ggagcgccac gcttgcccgg cctacggata attcaccctc ttctgtttga
    10561 agagggttat gatcactctg ccactttttt cttcgccgca cgcttcgctt tggtcgctgc
    10621 ggctattttc tgcgttttag ccgatggctt tttcgctttt ctcgcgtctt tcttcgccgc
    10681 tttcggcttc ggttttttct cgccgcggaa ggcactgtct ggctcaaagt ttaccttttt
    10741 accgacctga cgacgcttgc cgcctttttt gccagcatcg ccttttttcg ctttctcgcg
    10801 cgccgtttta ccgacgttgc gcggtgcgcg ttcgctggag atcaggctaa agtcgatttt
    10861 acgctcgtcc atattaaccg cttcgacgcg aacttccacg cgatcgccca ggcgataagt
    10921 ctggccgctg gattcaccca tcaggcgttg ccctacctgg tcaaagcgat agtagtcatt
    10981 gtccagcgaa gagacatgga ccagaccatc aatgaacaag tcgtccagac ggacgaagaa
    11041 gccaaagcca gtgacgctgg aaattacgcc tttaaagacg ttacctacct ggtcgagcat
    11101 gaagtcacac ttcagccagt cagctacatc gcgtgttgct tcgtcggcac gacgttccgc
    11161 catcgaacag tgctgaccca gttgcagcat ctcttccatc gaataatggt aaccaccggt
    11221 ttcagtggta ttgccctgat gcccctgctc tttcgccagc agatacttaa tggcgcggtg
    11281 cagcgtcagg tcaggataac gacgaatcgg cgaagtgaag tgcgcatagg actgcaatgc
    11341 cagaccgaag tgaccacggt tttccggatc gtaaatcgcc tgtttcatcg agcgcagcag
    11401 catggtttgc agcatttctg catcaggacg atcggcaacc gactctagca actccgcgta
    11461 atcacgcggt tccggcttgt taccgcccgg cagttccagc cccagctccg ccagcactga
    11521 acggaaagag gtaatcgctt cggtgctcgg cttatcgtga atacggaaca gtgccggttc
    11581 tttcgctttc tcaacgaaac gcgccgccga gatattcgcc agaatcatac actcttcaat
    11641 taatttgtgt gcgtcgttac gctgggtctg ttcgatacgt tcaatacggc gttcagcgtt
    11701 gaaaatgaac ttcgcttctt cgctctcaaa tgagatccca ccgcgttctt cacgggcttt
    11761 atccagcact ttatagaggt tatgcaactc ttcgagatgc ttaaccagcg gggcgtactg
    11821 ctcgcgcaga tcctgatcgc cctgcagaat atgccagact ttggtgtagg tcagacgtgc
    11881 gtgagagctc attaccgctt catagaattt gtagccagtc aggcggcctt tcgacgaaac
    11941 cgtcatctcg cacaccatac acaggcggtc tacctgcggg ttaagcgaac acagaccgtt
    12001 agagagcact tccggcagca tcgggataac ctgcgaaggg aagtacaccg acgtaccacg
    12061 gttacgcgct tctctgtcca gcggcgttgg agggcgcaca tagtagctga cgtcggcaat
    12121 cgcgacccat aaacgccagc cgccgccgcg ttttttctcg cagtaaaccg catcgtcaaa
    12181 gtcacgggcg tcttcgccat caatggtgac cagcggtaaa tcgcgcagat caacacggcc
    12241 cgcttttgct tcttccggca cttcttcttt cagcccggca acctgttgct caacagcctg
    12301 cggccagatg tacggaattt catgggtacg cagagcgata tcaaccgcca tgccggtgcc
    12361 catattgtcg cccagcactt cgacgatttt acccaccgct ttggtacggc gagtcgggcg
    12421 ttgggtcagt tcaacaacca ccacaaagcc catccgcgcg cccatgatct gatcgggcgg
    12481 gattaagata tcgaagctca gacggctgtc gtcaggaacc acaaagccga cgcccgcttc
    12541 ggtaaagtag cgaccaacaa tctggctggt ttttggcacc agtacgcgga caatacgcgc
    12601 ttcacgacga cctttacggt cagcgcccag cggctgcgcc agcacctgat cgccatgaat
    12661 gcaggttttc atctgctcgc tggagagata caaatcatct ttacgccctt caacccgcag
    12721 aaagccgtag ccatcacggt ggccaataac ggtacctttc accaggtcga ggcgttccgg
    12781 cagcgcatag cactgacgac gagtgaagac cagttgacca tcgcgctcca tcgcgcgcag
    12841 gcggcgacgc aggccttcaa gctgctcttc gccttcaatg tgcagttcta ccgccagctc
    12901 atcacggctg gccggttttt cacgtttggt taaatgttcg aggataaatt cccgactagg
    12961 gatgggattc gcgtattttt cagcttcgcg ttcctggaaa ggatcttgtg acatctcggt
    13021 tcctccgttg tcatctctga tgaagatttt cgtcactcca ccagcaataa tttataaagc
    13081 ggttgattct cttcaaccaa atcggcaagc gtgtagttat ccagttccgt aagaaaactt
    13141 tgcacggcct tagaaagtgc ctgtttcaac cgacaggcag gtgtaatgtg gcaaaactca
    13201 ctgctgcaat tcaccagcga taagggctcc agctcgcgca ccacatcacc aatacgtatc
    13261 gcactcgccg gtttacccag acgaatgccg ccattttttc cacgaacagc agtcacgtag
    13321 ccggcacgac taagttgatt gattattttg accatatgat tacgggagac gccgtagacg
    13381 tcagtcactt cagaaatact ggtcatccgc ccttctggca atgacgccat gtagatcagc
    13441 gcacgtaatc cgtaatcagt gaaactcgtt aactgcacat caacctcaaa agggaaatcg
    13501 ggaaaaaata catttatatt gatgataaac cagccacaag ctgtgtcgct aatttatttc
    13561 agttcgggga aggaaaaaag cgaggattta acactgtgtc ggaaagcgta atggcttaac
    13621 cgacacagca atgtatcaca ctgttagccc ggcaggcaaa atatctgcca ggcgtaccag
    13681 aattacgcgt cgaacgggtc gcgcagaatc atggtttcag tacgatccgg accggtagag
    13741 atgatatcga tcggcacacc ggtcagctct tcaatacgct tgatgtagtt cagcgccgcc
    13801 tgcggcaggc cgctacgatc tttcacgccg aaggtggatt cagaccagcc cggcatggtt
    13861 tcgtaaatcg gctctacacc tttccagtcg tcagctgcca gcggagtggt agtcacttca
    13921 cgaccatccg gcatacggta agccacgcag agtttcacct ctttcaggcc atccagaacg
    13981 tccagcttgg tcaggcagaa gccagacagg gagttcagct gtaccgcacg acgaacggca
    14041 acggtgtcca gccagccggt acgacgacga cgacccgtag ttgcgccgaa ttcgttaccc
    14101 tgcttgcaga ggaactcgcc agtttcatca aacagttcag tcgggaacgg acctgcaccc
    14161 acacgagtgg agtaagcttt gaggataccc agaacgtaat caacataacg cgggcccagg
    14221 ccggaaccgg tcgccacgcc accagcagtg gtgttggaag atgttacgta cggataagta
    14281 ccgtggtcga tatccagcag cgtaccctga gcaccttcga acatgacgaa atcgccacgc
    14341 tgacgcgcct ggtcgagcag gtcagaaacg tcaaccacca tagaagtcag gatgtcggca
    14401 acagccatcg tatcatccag aactttctgg taatcaaccg cttcagcttt gtagtagtta
    14461 accaactgga agttgtgata ttccatcact tctttcagtt tttcagcgaa ggtttctttg
    14521 tcgaaaaggt cgccaacacg cagaccgcga cgtgccactt tatcttcata agcaggcccg
    14581 ataccacgac cggtggtgcc gatcgctttc gcgccacgcg ctttctcacg cgcgttatcc
    14641 agcgcaacgt gataatcaag gatcagcgga catgcttcag acagcagcag acgctcacga
    14701 acggggatgc cacggtcttc cagttctttc atctctttca tcagcgcggc tggagacagc
    14761 acaacaccgt taccgatgat gctggttaca ttctcgcgga gaatacctga tggaataaga
    14821 tggagaacgg ttttttcacc gttaattacg agagtatggc ctgcgttgtg accgccctgg
    14881 tagcgtacaa catatttagc ccgttcagtc agaagatcga cgatcttacc tttaccttcg
    14941 tcaccccatt gggtgcccag tacgacgacg ttgttaccca tttttcaaaa tcaccgtttg
    15001 cttaaaaatg gattctacca tcgctttttc agagttacag cactttttgc actcaaaaat
    15061 gacctcgatc agtctgtttt ttgttcagcc aatcgttttc ctcaacatgt agtagaccac
    15121 aacgcccgca accacaagtc caccgccaaa acgacgtaaa atattatcag gcaaattggt
    15181 catcgcagag atcatcttct tccatgcctt cgggtaaagc atcggcccta aaccttcgag
    15241 caccaaaacc agggcaagcg ccagccagat tgtcgaattc attcattatc cttatagaaa
    15301 aagaaaacca ccgacatccc tgaggatgcg gtggctttat tgacctgtac cgcagtcgtt
    15361 atattaacgc gttgcggaag tcggcgtctt catgtagcgg aagaaatcgc tatccgggct
    15421 catgaccatc acgtcctgat tgccagagaa gctgttctca taagcacgca ggctacggat
    15481 gaatgcgtag aagtccggat ctttactgaa tgcatcagca aacagtttgg ctgcttcggc
    15541 atcgccttca ccacgcatga tgcggccctg acgctcagct tctgccagcg ttctggtcac
    15601 ttcatagtcg gcagtcgcgc gcagtttttc cgcttcttcc tgaccttgtg aacggtgacg
    15661 acgcgctacc gcttcacgct cggcgcgcat acggttgtag atcgcttcag acacttcggt
    15721 cggcaggttg atctgcttga tacgcacatc gacaacttca atacccagcg ccgccatact
    15781 gttcgggttg atgaccggaa ctttgccctt cgtctcagcc gttacgcgct ctgccgcttc
    15841 ggcaatggcg ttatctgctg ccggggtagt aacttcatct tctgtacccg cagaaccgga
    15901 gttcagcgcg tcacgtactt cgagggtcag acgaccacgg gaatcggtga cgatgtcttt
    15961 cacatccaga cgaccaattt cagaacgcag acggtcagag aacttacgtt tcaacagcac
    16021 ttccgcttgc gaaatgtcgc caccgcccgt tgccaggtag taacgactga aatcgctgat
    16081 gcgccatttg atgtaagagt cgacgatcag gtctttcttc tctttggtca caaagcggtc
    16141 ggcctggttg tccatggtct gaatacgtgc gtcgagcatt ttcaccgttt caatgaacgg
    16201 tatcttgaaa tgcagacccg gctcataaac cagaggtttg ttgtcatcgt cacgcagtac
    16261 cttaccaaaa cgcagcgtaa tgccgcgctc accttctttg acgacaaaga cagacatgta
    16321 aagcactacc agcacgatga tgataatcgc gataactgac ttacgcatcg ttattccccc
    16381 tgacgctggt agtcgttacg ctgcgcgttg gcgcggcgtt ggtccataat atcgccctga
    16441 ctggtggacg acgtgttgct tgctccactg gttgtggagg aagaggctgg cggcagacgc
    16501 agcagattgc tggcaccgtt atcgctcttc gccgcagggg cgttaccacc tttcagcatc
    16561 tggtctaacg gcagaaccat caggttgcca cctttatcgt taaccagcac tttgcgggtg
    16621 ttgcccaaca ctttttccat cgtctcgata tacagacgct cgcgagtaat ttccggcgcg
    16681 gctttatatt ccggcagaag tttagcaaag cgcgccactt caccctgagc ttccaggatg
    16741 gtctgggcct tgtacgcacg cgcctcttcg aggatacgtt gcgcctgacc gttcgcacgc
    16801 ggctgaactt cgttggtata cgcttctgct tcacgaatat attgctgttc gttttcacgc
    16861 gcggcaatcg catcgtcaaa cgccgctttt acttcttctg gcggacgagc tgcctggaag
    16921 ttgacgtcca gcagagtgat acccatgtca tacggacgaa tcgtctcttc cagttcgcgc
    16981 tgagtatcgc tacgaatcac ggtacgacct tccgtcagaa tgcggtccat ggtgtattta
    17041 ccgataaccc cgcgtagggc gctgtcggta gcctgacgca ggctgtcatc cgggctggtc
    17101 acgctataca gatatttttc cggattggtg acgcggtact gcacgttcat ctcaacgcgc
    17161 actacgttct cgtccgacgt cagcatcaca ccagaagcgg ccagttcacg cacggcttcc
    17221 acgttcaccg gtttgacttc gtcgataaac gtcggtttcc agttcagacc cggctcaacc
    17281 agatggctga atttaccaaa gcgtgttacc acgccacgtt cggcttcttt aatggtatag
    17341 aaaccactgg ccgcccagat aatgacaatc gctgccgctg cgatggtaac gacacgaccg
    17401 ccaagctgcg ggcgcgggcc ttgcgatgaa ctgccaccgc cagatccggt gcctttaccg
    17461 ccgcccagac caccgagctt tttgctcagt ttgcggaaga tatcatctaa atcaggtggc
    17521 ccttgatcgc gaccgccttt gtttccattt ccctcagagt tgccgccagg tttgctgctt
    17581 ccccacgggt cgcggtcttg tccgttatta ccgggctgat tccacgccat gtttgtgctc
    17641 catatttgtt atgcggtgat ccctgttgtc tttcgacgcc agaggatatg actccacgct
    17701 tcagacgcta cgccgttaga tcaggtaatc gatcaacgcc ggttcttgtt tacagaggcg
    17761 acgccagtca acgatcggca tacgaacttg cagacttacg ctgccgtcct cctccatcca
    17821 ctctttttct attgcctgaa gctgataaaa acgacttctc agacgccctt cctgcggtgg
    17881 cagacgcaat gtatgctgcg ccacctcgcc ggaaagccgc tccgtcaaag cctgaaaaag
    17941 ctgtggtatc cccgctccgg tctgtgcgga aagccagaca cggatcggtt tgttctcttc
    18001 gtcccgatca atacgcggtt cgaaatcttc cagcatatcg atcttgttca tcaccagcag
    18061 ggttgggatc tcgtgagcgt cgatctcttc aagaaccgta ttcaccgctt cgatgttttc
    18121 ttgtacacgt acatccgccg catcaatgac gtgcagcagc aatgtggctt gccgcgtctc
    18181 ttgtaacgtg gctttaaatg ccgccaccag atcgtgcggc aggtggcgaa taaaccctac
    18241 ggtatctgcc aacacggttt caccgacatc cgcgacgtca atacgccgca acgtcgggtc
    18301 gagggtggca aacaactggt ctgccgcgta gacccgcgct tcggtgatgc gattgaaaag
    18361 ggtagattta ccggcgttgg tatatcccac cagcgaaaca gtaggaacgt cggctttgat
    18421 acgcgattgc cgcccctgct cacgctgctt ttcaactctt tccaggcgcg actgtatctg
    18481 cacgatgcga ttacgcaaca aacgacggtc ggtttcgagc tgggtttcac ccggaccacg
    18541 caaaccaatc ccgcctttct gtctttcaag gtgggtccag ccacgcacca ggcgcgtagc
    18601 cagatggcgc agctgcgcca gctcaacctg caacttaccc tcatgggtac gcgcacgttg
    18661 ggcgaaaata tctaaaataa ggccggtgcg gtcgatgaca cgacactcgc acaaacgctc
    18721 caggttacgc tcttgcgccg ggctcagggc atggtcaaaa agaacgaccg aagcacccgt
    18781 cgctttgaca gcttccgcaa tttcaactgc tttaccttca cctacaaaat actttgggtg
    18841 cggcgcttta cggctaccgg taatcacctg caatgcttcg acaccggcgg aagagaccag
    18901 agattcaaac tcctggagat cttccatatc tttgtcttgc gtaaaataga tgtgtaccag
    18961 taccgcctgc tcaccagcat cataacggtc aaacaagcgt ataaccctct aaatagatca
    19021 gcggggaacg caggatcgct ggctccccgt gtaaaaaaaa cagcccgaaa ccttattcgg
    19081 tttcttcgct gtcctgttgc gcggaagtat tctgcgcgct gctaccatga tggtagttac
    19141 tgctggtacc gccaccggcg ttgttactgt gatgagaaac cgggcgagac gggacaacag
    19201 tagaaatcgc gtgcttgtaa accatctggc tgaccgtgtt tttcaacagg atcacgaact
    19261 gatcaaaaga ctcgatttgc ccttgcagct taataccatt caccaaataa atagaaactg
    19321 gaacacgttc ccgacgcagt gcgttcagga acggatcttg taaagattgc cccttagcca
    19381 ttctctcttt tccttatatg cttatttgta ctttgaacct ttcgattctg aaaaaattgc
    19441 gcacgatacg tctcaattgt acacattcag cctgcgatag caccaacaac ctgtaatact
    19501 tcgtcacgcg cctgttctgg tttttcacta tcaagccagt gaaccccttc ccaaccacgc
    19561 agccaggtta tctgccgctt cgccaactgt ctcgtggcgc aaacacctcg ataaaccatt
    19621 tcatcgtatg agatttcgcc ttcaaggtaa gaccacatct ggcgataacc cacgcaacga
    19681 atggaaggca agtccgtatg caaatctcct cgggcaaaaa gcgcccggac ttctgcttca
    19741 aaacctgaag ccaacatctg atgaaaacgc tgctcaattc gttgatggag cagttcacgg
    19801 ctcgccgggg cgatggcgaa ctgatgcacc tgatacggta gagcgtctcc tgacgtttgc
    19861 gtcagttccg ttaaagtttt acccgaaatg aaaaaaactt ccagtgcccg ggaaagcctt
    19921 tgtggatcat ttggatgaat ccttgctgcc gcaaccggat cgatctcctg aagttgacga
    19981 tgcaatgact cccaaccttg ctctgccgcc tgttgctcaa ttctggcccg cacttccggg
    20041 tctgccgacg gtagcggcga caacccttcc agcaatgcct tgaaatacaa catcgtaccg
    20101 cccactaaca gtgggatccg ccccgccgcg gtgatatcgg ccatttccgc cagcgcatca
    20161 cggcgaaaat cagcagccga gtaagcctgc gacggatcgc gaatatccag caatcggtgc
    20221 ggcgcggcga gtaactcttc agcgttcggc ttcgccgtcc cgatatccat ccctttgtaa
    20281 ataagggcag aatcaacgct tatcaactct actggtaaaa ttttacgcag ctcaatggct
    20341 aacgccgttt taccggaggc cgtcggcccc atcaaaaaaa tcgccttagg caggctcgcc
    20401 ttactgatat cactcatctt tcagggcttt tatcgccgga tgtaaatcaa cagattgtaa
    20461 cagaccaccc ggcggcgttt tcacaagttg cggacataac cgttccacgt ccgccagcag
    20521 agttatggcc tgcgccattg accactgcgc atgttcgctc atcagatttc gtgcaatcca
    20581 ctgcgcaata ttgccaggtt cgaatacgga ctgcttcgcc aggtagccta tcagttcagg
    20641 aatcaagatt tgtaaatttt gttggcgtaa gggtaaaggc acggccctga tggtcacatg
    20701 ctgtgcatct gactggaaat caatacccaa ttccgccagg gcagactgcg ctttttctaa
    20761 tgccgatttt tcttcgccag aaacttttag ccgcaacgga atcagcagcg gctgggcgca
    20821 aactggcgct tcacccggcg tcagttgcgc ctgacgcagc caacgttctg ccactggcaa
    20881 ggctaaaagt gaaatgttgc cgccgcgctc cagcaacgca cagtcggaat ggacgatagt
    20941 cagtacccga ccaaaactct gactgttcgc cgcaagtgca ggttcctgcg gttctggcgc
    21001 ttttggtttt tgcatcggcg cgggcgtttg caaaagctgg cgatacactt caccttgctg
    21061 tttctggtaa cctggctgcg cattcggcca gggggcagcc ggacgactgc ctgaggcggg
    21121 cgcaggagtg taacgcggag ctactggctc acgaactgcc ggttcagcaa aatgattgcg
    21181 ccccgccgcc acgcggtttt ccgggatggc gcgcggtgca ggttgtggtt catcgtccag
    21241 cggtagcggc gtttccagtt gctgttgtag cacgctcagc acgccctgat agataaaatc
    21301 atgcaccaga cgcgactgat ggaaacgcac ttcgtgtttg gcagggtgca cattgacgtc
    21361 cacctgatgt gggtcgatct ccagatacag cacaaatgcc ggttgctgat cggcccccag
    21421 tttgtcttca caggcctggc ggatcgcgtg attgatcagg cgatcgcgca tcatgcgacc
    21481 attcacatag caatactgaa tttccgccag tgcgggcgtg gtgtgattcg gatctgccac
    21541 ccagccgcgc agcgtaagat cgccgtgttg ccattcaatc gccagcgctt gttcgagaaa
    21601 agcggtaccg caaatcgcgc ctaagcgccg ttctttttgc ccgccttccg gcactgcgcg
    21661 gtactgacgc acaattttac cgttatgcga caggttgatc gtgacgtcga aacgcgccag
    21721 cgcaatgcgg cggatgatct catcgatatg gttaaattcg gttttctcag tgcgcaggaa
    21781 tttgcgccgc gccggagtgt tgtagaacag atccagcacc tccagcgtcg ttcccacagg
    21841 atgcgccgcc ggttttaccg tcacgtccat atcgcgccct tcggcatagg cctgccaggc
    21901 ttcctgctgt tctgcggtac gtgaagtgag cgtcaggcgg gaaaccgaac tgatactcgc
    21961 cagcgcctca ccgcgaaagc ccaggctgat aatggcttcg agatcgtcca gagaggcgat
    22021 tttactggtg gcatgacgag ccagcgccag cgccagctca tcttttttga taccgcagcc
    22081 gttatcacga atgcggataa gtttcgcccc accgcgttcg atatcaatat cgatacgcgt
    22141 cgcacctgca tcgaggctgt tttccaccag ttctttgact accgacgcag gtcgctcgac
    22201 cacctcacct gcggcaatct ggttcgccag ttgtggcggt aagacctgaa ttggcatcaa
    22261 tcgtccttag tttggcagcg tgcgatctgg cgtcgtcacc gtactggcag tttgtgccgc
    22321 tgtaccctgc ggcgcagatt gcatcggatg cgcaaggaaa taattacgca ggcctttgta
    22381 aatggcttct gccagctgtt gttggtaatc gtcgctcgcc agcaaacgtt cttcgctgtt
    22441 gttgctgata aaaccggttt cgaccagtac tgatgggata tccggcgaac gcagaacgcc
    22501 aaggctggcg tgttctggtc gacgtttatg tatttcgcca atgcgttgca actggctgat
    22561 catactggtc gctacatcat accctacacg ctgggaatga ccgaactgta aatccagcac
    22621 cgcctggctt aaatagggat cagactgact gttcgccagc acatcgcccg ctccgcccag
    22681 cagctccgac tgtttctcat gctgttccag ccagcttgcc atctcgctgt ttgcacgacg
    22741 gtttgagagc acccatacgg aagcgccagt cgcactgcgg ttcggtgcgg catcagcgtg
    22801 aatcgacacg aggaaattgg cgttttgctt acgtgccaca tcgctgcgcc ccatcaccga
    22861 gataaagtaa tccccgtcac gggttaaaac gcctttaaac attggatcgt cattgagcaa
    22921 agtacgcaat ttacgtgcga tggcgatggt gacatttttc tcccgcgtac cgccggggcc
    22981 gatagcgcca gggtcctgac cgccgtgtcc tgcatcaata gcgatgataa ttttatcgcc
    23041 agtgttagcc gtcgcacgcg ctgccggacg cgttaccgta ttactgctga taacacctgt
    23101 agtgcggtta ctttccgttt taaacggatt gcgcgccggt tcgctgacgc gcggtgcgac
    23161 aaccgcaggc gtttcaacgc gtttcgcaac cacaggcggc ggaggaggcg gtggcggcac
    23221 atcggcgtta atcgtaaaga cgacagtgta attgctgcca ttctgccgct tcaccgcttc
    23281 ggttttaccg ttttcggtaa gatcgaccac cagccgtagc gtttgtgcat ctttaggcgt
    23341 tccggagcga atcgccttca ccagattatt gccgctaaac aacaacggca gcccctgaat
    23401 tacccccgtt tgtttgatat cgagcgccac ggtgcgtttg ctttgatggc taaacgcata
    23461 atcaggatcg ccaataaaac tcaacgttat ccgcgcctgt tggttgccgt tagaaacctg
    23521 aatatcagag agcgtcgcgg cacccacctg cgcgcacagc agcagcaacg tcgctaccaa
    23581 ccaatttctg atgcgataca tcatcccgcc acctttcaaa ggttaaccgg ctaaacgcgc
    23641 cagcaacaat tcacccgcag aggaaaccgc actcacgcgc gcctcacggc cttgtgcctg
    23701 ataatcaatg tgtatttcga catccgggtc aggaagaaca cctgtacctt gttgtggcca
    23761 ctccaccagg cagatggcat cgttggcaaa ataatcgcgg atccccataa actccagctc
    23821 ctcgggatcg gcaaggcggt acaaatcaaa gtgataaacc attaaattgt cgagcgtata
    23881 gggttcgacc agcgtataag tggggctttt gacattaccc tgatggccca aagcctgtaa
    23941 aaagccccga ctaaaggtgg ttttacctgc gcctaaatcg ccatacagat agattacggt
    24001 tgcgccatcg caggctttcg ctacccgctc gcccaggtct aatgttgcct gctcatcagg
    24061 gagcggaatt actcgattca tcatggtttt tatcagtcac ttccgggtta acaatacgct
    24121 gtagcgtgga aaagagatcg gttgccagca tcccgcgcgt tccaaaacgc gccgccagta
    24181 cgtcagctgc cgcaccgtgc gcgacgcagc ccgcacaggc tgcatcatac ggcgacagtt
    24241 tttgcccaag caatgcgcca ataataccag agagcacatc gcccatgccg ccgctcgcca
    24301 tgcctgcatt cccggcatca ataatgccta aagcgtcagg atgggcggcg accacggttc
    24361 cggcaccttt cagcaccgct acgccgccat aacgttgtac cagacgttta gcgcaatgta
    24421 agcggtcact ttcaatttca gcgacggaac agcctaacaa ccgtgcggcc tcgccaggat
    24481 gcggcgtgat cacgcgattg tgacgcttat cgggattgat tgccagtagg ttcaatgcat
    24541 cggcatccca caacatcggt ttgcgaaaat tctcaacttt ttgcagggct tttttccccc
    24601 actcttgctg gcccagtccg ggaccaatca ccagcacatc ggcccattcc aggctttcgg
    24661 taagagagtc catagtcagt tcatgcacca tcaattctgg tcgtgcagtc agtagcggcg
    24721 caatgttttc actgcgggtc aatactcgaa ctaaaccagc accagcacgc agtgccgctt
    24781 cccccgtcat acgaatagct cccgccgtgc cgtgatcgcc gccaataatc accactctcc
    24841 cgtgatcgcc tttatgcgat gtcgggcgac gcggtttcag ccagtgagaa agttgttctg
    24901 ccgaaaaccg ctgaattttc gtctcctgac ctgccagcca gctatccagc cccagtgagt
    24961 caaaatgcag ttgtccggta acatcccgcg cttttccagt gagcaagcct ggtttcagcg
    25021 caataaaagt gatggtgtga tcggcgttga tcaccgcgcc tggcgtagcg ccagtttcag
    25081 ccagcagccc ggaagggata tcaaccgcca caatcggcgc aggatgggta ttagcgtggt
    25141 cgattaactg gctaatggat tcgcggggtg cttgctgcaa gccggtaccg agcagcgcat
    25201 caacaatcag atctaccgat tcgggccaga cagtattcga agcatggatc tcgcctcccg
    25261 cgtttaacca tgcttcgcgt gacagcgcgg cctcttccgg caacggtttg tcactttcct
    25321 gggccagcag cgtgacctca ataccgaccg ctttggccag tcgcgcgacc acgtagccat
    25381 cgccgccgtt attaccatga ccgcacagca ccagccagtg gcgggcgtca ggataagccg
    25441 tgcgacacac ctggaatgcg gcttcgccag cgcgaagcat cagctcatag agtgtgagtc
    25501 ccagcgcatc tgccgcctcg cgttctccgc ggcggatatc gtcggcgtac cagacggtgt
    25561 gtggtatact tacggggttt ttcttcattg tatggtccgt catgtcagag cccctcgatc
    25621 tcaatcagtt agcgcaaaaa attaaacagt gggggctgga actgggcttt cagcaggtag
    25681 gtattaccga taccgatctc agcgcgtccg agcccaaact gcaagcatgg ctggacaaac
    25741 aataccacgg cgaaatggac tggatggcgc gtcacggtat gctgcgtgct cgtcctcacg
    25801 aattattgcc cggtacgctg cgcgtgatca gcgtgcggat gaattacctt cctgctaacg
    25861 ccgcatttgc cagcacgctg aaaaatccca aactcggcta tgttagccgt tatgcgctgg
    25921 gccgtgacta tcacaagctt ctgcgcaacc gactcaaaaa gctgggcgag atgattcagc
    25981 aacattgtgt ttcgctgaat tttagaccgt ttgtcgattc tgcgcctatt ctcgagcgcc
    26041 cgttagctga aaaagcagga cttggctgga caggtaagca ctcacttatc ctcaatcgcg
    26101 aggccggttc attcttcttt ttaggcgaat tgctggtcga tattcctctg cccgtggatc
    26161 aaccagtcga ggaaggatgc ggtaaatgcg tggcctgtat gacgatttgc ccgaccggag
    26221 ccatcgtcga gccatatacc gtcgatgctc gccgctgtat ctcttatctc accatcgaac
    26281 tggagggggc gatcccggaa gagttgcgac cgttaatggg aaaccgtatt tacggttgcg
    26341 atgactgcca gcttatctgc ccgtggaatc gctattcgca actcactaca gaagacgatt
    26401 tcagcccgcg taagccgcta cacgcaccgg aactcattga gttattcgcc tggagcgaag
    26461 agaagttttt aaaagtcacg gaaggatcgg cgattcgccg tataggtcac ctgcgctggc
    26521 tgcgtaatat cgccgtggca ttaggcaatg ccccctggga tgaaacgatt ctggcggcgc
    26581 tggagagtcg caaaggtgag cacccacttc ttgatgagca catagcgtgg gcgattgcgc
    26641 agcaaataga gagacgaaat gcgtgcatag tcgaagtgca attgccgaaa aaacagcgtc
    26701 tggttcgggt gattgaaaaa gggttaccgc gtgacgcctg attcatccac agcttgtgaa
    26761 taaaaataaa aacgcattgc aattcaagac gaataaaatc gtcaagtgat cgactcgaca
    26821 aaacgaaatc ttaattttta acttatctat caatagatta aaaaaatact attcaccgtg
    26881 cgaagtttct ttgaaattaa gattacggcc tgaggctgtg gataactctg tttacaagag
    26941 tttgtcagag acaacaaaaa ccagcaataa cgcatcgcgt cgctgtggat attttattga
    27001 gagaagaatt tggagcggga aacgagactc gaactcgcga ccccgacctt ggcaaggtcg
    27061 tgctctacca actgagctat tcccgcttgg gtggtctgtg ctttacagca ctttcaaatt
    27121 ttggagcggg aaacgagact cgaactcgcg accccgacct tggcaaggtc gtgctctacc
    27181 aactgagcta ttcccgctta atcttcgtct ttcgaaccgc tactgccttg gctgccttca
    27241 ctcaccttag tcacttactt ttgtaagctc ccaaggattc gttcagttgc cgccttgtcg
    27301 caattcgaaa tactgcgatt gggtggtctg tgctttgcag cacttttcaa attttggagc
    27361 gggaaacgag actcgaactc gcgaccccga ccttggcaag gtcgtgctct accaactgag
    27421 ctattcccgc gtactactta attttgcttt cgtaattttc gcatttctgc gtcgttacgg
    27481 gaggcgcatt atacgagaaa tctttccctc tgcaaccccc ctaaaaaatt ttttttgagt
    27541 ttttatctca aatgctgaaa ttatcagcaa gacgttcaat ttagcgtcaa aatcacgccg
    27601 ccgcaagtgg attccggcgc ttacgtgacc aggaaaaatt ttacagcttg ataaaatgct
    27661 cgcgatagta agccagttct gccaccgact cacggatatc atccatcgcc tgatgcgtcc
    27721 cctgcttggt aaaaccatca agaatttccg gcttccagcg gcgcgccagc tctttcagcg
    27781 tgctgacatc gagataacgg tagtggaagt aggcttccag ctccggcatg tatttaaaca
    27841 ggaagcgacg atcctggccg atgctgttac cgcaaatcgg cgattttccc gcaggcaccc
    27901 actgttttaa gaattcgagc gttgccagtt cagcttcacg atcgcccatt gtgctcgctt
    27961 tcacgcgctc caccagcccg ctggcggtat gggtgcgcac gttccagtca tccatcagcg
    28021 ccagctgttc atcagactgg tgtactgcaa tggtcggccc ttctgccaga atattcaggt
    28081 tggcatcggt caccagcgtg gcaatctcaa taatgcgatc gcgctcggga tccagaccgg
    28141 tcatctcaag atcgatccaa atcaggttgt tttcattggc actcatgcta ttttccaccc
    28201 attcacagtg actatattga tccacaattg cgtgtatcat agatgttttg cccatcaggg
    28261 gcgaccagga gtctgtacga ttgagtaaaa ataaactctc caaaggccag cagcgccgcg
    28321 tgaacgccaa tcaccagcgt cgtcttaaaa cgtctaagga gaagcccgac tacgacgaca
    28381 atctgtttgg cgagcctgat gaaggtatcg tcatcagccg ctttggtatg cacgctgatg
    28441 tggaatccgc cgatggcgac gttcaccgct gcaatattcg ccgtaccatc cgttcgctgg
    28501 tgaccggcga ccgcgtagtc tggcgtccgg gtaaaccggc ggcggaaggc gtaaatgtca
    28561 aagggatcgt ggaagcggtg catgagcgca cctcggtgtt gacgcgcccg gacttctacg
    28621 acggcgtgaa acctattgcc gccaacatcg accagattgt tattgtctcc gccattttgc
    28681 cggagctgtc gctcaatatt atcgaccgtt acctggtggc ctgcgaaacc ttgctgattg
    28741 agccgattat tgtgctcaac aagatagacc tgctggacga cgaaggcatg gagttcgtca
    28801 acgaacagat ggatatctac cgcaacattg gttatcgcgt attgatggtt tccagtcata
    28861 ctcaggatgg gctgaaaccg ctggaagagg cgttgaccgg acgcatcagc atttttgccg
    28921 ggcagtctgg cgttggcaaa tccagcctgc ttaacacgtt gctggggctg caaaaagaga
    28981 tcctgaccaa cgatgtttct gacaactcgg gtctcggtca gcacaccacg accgccgctc
    29041 ggctgtatca cttcccgcac ggcggtgatg tgattgactc ccctggtgtg cgtgagttcg
    29101 gcctctggca cctggagccg gaacaaatca ctcagggctt tgtcgaattc catgactact
    29161 taggtctgtg taaatatcgc gattgcaaac acgataccga tccgggctgc gctatccggg
    29221 aagcggttga ggaagggaaa atcgcggaaa cccgtttcga aaactatcac cgtattctgg
    29281 aaagcatggc gcaggtaaaa acgcgtaaaa acttttctga tacggatgac tgacaattaa
    29341 gctaaccgtc gttagaatcg tccccttttt ttcaggatcc ggcatgtagg ccggatcagg
    29401 aacgacaaaa caatggcctg gaggctacct tgttaaattc atttaaactt tcgctacagt
    29461 acattctgcc gaaactatgg cttactcgcc tggcgggttg gggcgcaagc aagcgggcag
    29521 gatggctgac aaaactggtt atcgatctgt tcgttaaata ctacaaggtc gacatgaaag
    29581 aggcgcaaaa gccggatacc gccagctacc gcacctttaa cgaattcttt gtccgtccgc
    29641 tgcgtgacga agtacgccca atcgataccg atccgaatgt actggttatg cctgccgatg
    29701 gcgttatcag ccagttgggt aaaatcgaag aagataaaat cctgcaagcc aaaggccaca
    29761 actacagcct cgaagccctg ctggcaggca actatctgat ggcggacctg ttccgcaacg
    29821 gtacgtttgt gaccacttac ctctccccac gtgactacca ccgcgtacac atgccgtgca
    29881 acggtattct gcgtgaaatg atctacgtgc cgggcgatct cttctccgtt aaccatctca
    29941 ccgctcagaa cgtgccgaat ctgtttgccc gtaacgaacg cgtgatctgc ctgttcgata
    30001 ccgaatttgg cccgatggcg cagattctgg tcggtgcgac gattgtaggc agcattgaga
    30061 cggtctgggc gggcaccatt acgccgccgc gcgaaggcat catcaagcgc tggacctggc
    30121 ctgccgggga aaacgacggt tctgtggcac tgctgaaagg ccaggaaatg ggtcgcttta
    30181 aactcggttc caccgttatc aacctgtttg caccgggtaa agtgaatctg gttgagcaac
    30241 tggaaagcct gtctgttacg aaaattggcc agccgctggc agtatctacc gaaacctttg
    30301 ttacgccaga cgctgaaccc gccccgcttt ctgctgaaga gatcgaagca gaacacgacg
    30361 ccagcccatt ggttgacgat aaaaaagacc aggtctaatc catcaaagga aacgctgacg
    30421 tgcgcctgat tatcactttt ctgatggcct ggtgcctcag ttggggggcg tacgccgcga
    30481 cggcccccga tagcaaacaa atctcacaag aactggagca ggcaaaagcg gcgaaacccg
    30541 cgcaaccgga agtcgtagag gcgctccagt ctgccttaaa tgcgcttgag gaacggaaag
    30601 gttcccttga gcgcatcaag caatatcaag aagtcattga taattatccg aaactctccg
    30661 ctactctgcg cgcgcaatta aacaacatgc gtgacgagcc gcgcagcgtg tcaccgggaa
    30721 tgtctaccga cgcgctgaat caggaaattc tccaggtcag cagccagttg ctggataaaa
    30781 gccgtcaggc ccagcaagag caggaacgcg cccgcgagat tgccgattcg ctgaatcaac
    30841 tgccgcaaca gcaaaccgat gcccgccgtc agttaaatga aatcgagcgc cgcctgggga
    30901 cgcttaccgg caattccccg ctcaatcagg cacaaaattt cgcgttgcag tctgactccg
    30961 cacgcctgaa agcgctcgtc gatgaactgg aactggcgca gctgtccgcc aataaccgcc
    31021 aggaattagc gcgcttacgc tcagagctgg cggaaaaaga gagccagcaa ctggatgcgt
    31081 atttgcaggc cttacgtaat cagttgaaca gccaacgtca gcttgaggcg gagcgggcgc
    31141 tggaaagtac cgaattgctg gcagaaaaca gcgccgattt gccgaaagac atcgtcgcgc
    31201 aattcaaaat taaccgcgaa ctatcagcgg cgctgaatca acaggcgcag cggatggatc
    31261 tcgttgcctc gcaacagcgt caggctgcca gccagacgtt acaggtccgg caagcgttga
    31321 atacgctgcg tgaacagtcg caatggctgg gatcgtccaa tctgctcggc gaagcgttgc
    31381 gggcgcaggt ggcacggctg ccggaaatgc caaaaccaca acagcttgat accgaaatgg
    31441 cgcagttgcg tgtgcaacgg ttacgttatg aggatctgct taataaacag ccgcagctac
    31501 ggcaaatcca tcaggccgac ggtcagccgc tgaccgccga gcaaaaccgt attctggaag
    31561 cacagctacg cactcagcgt gagttgctga actcattgtt gcagggtgga gacacgctac
    31621 tactggaact gaccaagctg aaagtctcca acgggcaact ggaggatgcg ctgaaagagg
    31681 tgaatgaagc gacgcaccgc tatctgttct ggacctctga cgtgcgcccg atgaccatcg
    31741 cctggccgct ggaaatcgcc caggatctgc gtcgtctcat ttcgctggac accttcagtc
    31801 agttgggcaa agccagtgtg atgatgctga ccagtaaaga gacaatttta ccgctgtttg
    31861 gcgcgttgat tctggtcggt tgcagtattt actcacgccg ctatttcacc cgttttcttg
    31921 aacgttcggc ggcgaaagtc ggcaaagtga ctcaggatca cttctggctg acgttacgca
    31981 ctcttttctg gtcgattctc gtcgcgtcac cgctaccggt gctgtggatg acgctgggtt
    32041 acggcttgcg cgaggcgtgg ccttatccgc tggcggtcgc gattggtgat ggcgtaacgg
    32101 ccaccgtgcc gctgctgtgg gtagtgatga tttgcgccac ctttgcccgc ccgaacggct
    32161 tgtttatcgc tcattttggc tggccgcgcg aacgggtttc ccgtgggatg cgctactacc
    32221 tgatgagcat cgggcttatt gtgccgctga ttatggcgct gatgatgttc gataacctcg
    32281 acgaccgtga attctccggt tcgctgggac ggctttgctt tatcctcatt tgcggtgcgc
    32341 tggcggtggt cactctcagt ctgaaaaagg ccgggatccc gctgtatctc aacaaagaag
    32401 gcagcggtga caacattacc aaccatatgc tgtggaacat gatgattggc gcaccgctgg
    32461 ttgccattct ggcgtcggcg gtgggttatc tggcaacggc gcaggcgctg ttagcgaggc
    32521 ttgaaacctc ggttgccatc tggtttttgc tactggtggt ttatcacgtt atccgccgct
    32581 ggatgctgat ccaacgccgc aggctggcgt ttgaccgggc gaagcatcgc cgggcagaga
    32641 tgttagcgca acgtgcgcgt ggcgaagagg aagcacatca tcacagtagc ccggaagggg
    32701 caattgaagt cgatgaaagc gaagtcgatc tcgatgccat cagtgcgcag tccttgcggc
    32761 tggtgcgctc aattttgatg ttgatcgccc tgctttcggt cattgtgctg tggtcagaaa
    32821 tccattccgc tttcggcttc ctcgaaaata tttcgctgtg ggatgtcacc tccacggtac
    32881 agggcgtaga aagtctggag ccgattaccc tcggtgcggt gctgattgcc attctggtgc
    32941 ttatcatcac cacgcagctg gtgcgcaacc tgcccgcgct gctggaactg gcgattttgc
    33001 agcacctgga tttaacgccg ggtacaggtt acgccatcac caccatcacc aaatatctgc
    33061 tgatgctgat tggcgggctg gtcggcttct caatgattgg tattgagtgg tcgaaattgc
    33121 agtggctggt cgccgcgctc ggtgttggtc tcggttttgg tttgcaggaa attttcgcca
    33181 actttatctc tggcctgatc atcctgttcg aaaaaccgat tcgcattggc gatacggtaa
    33241 cgatccgcga tcttactggt agcgtgacga aaattaacac ccgcgccacc accatcagcg
    33301 actgggaccg taaagagata atcgtgccga acaaggcgtt tattaccgag cagtttatca
    33361 actggtcgct ctctgactcg gtcacgcgcg tggtgttgac gattccggcc cctgccgatg
    33421 ccaacagtga agaagtgacg gaaatcctgc tcaccgcagc gcgtcgctgc tcgctggtga
    33481 tcgacaaccc ggcaccggaa gtcttcctgg tggatctgca acaggggatt cagattttcg
    33541 agctgcgtat ttacgccgct gagatgggtc accgtatgcc gctacgccat gagatccacc
    33601 agctaattct ggcaggcttc catgcccacg gtatcgatat gccattcccg cccttccaga
    33661 tgcgtctgga aagcctgaat ggtaaacaaa cggggagaac attaacttct gcgggcaaag
    33721 gtcgtcaggc gggaagtttg taaggttttg tgccggatga acccatccgg ctcacaaaca
    33781 aaactgatag cgcagaaaaa actgccacag ccagtagccg aaaatagcca gcgcggtggc
    33841 gtacataaac cagccgcgtt tttttcgcca caaagcgaac cagagaaaag ggaaaaagag
    33901 cggtaacgtc attatcacac cgacatcgcg agtatcgtca tcttcgagat acatcaaaac
    33961 atcacagacg ttgttaatcc cgcgtccatc aatcatccac tctttgtcca gcgcggaggt
    34021 aatggttatc cagaggaacg ccacaatgaa ccagatgcag cacaagacaa acatgcgcgc
    34081 actcatgaga taacccgtag tttaatttcg ctatagcaca tacgaaatcc cactaattcc
    34141 ctgtcattgc gccctatcgt cagacagacg atagggcatc gggttattcc accacccact
    34201 gcggattttt tgccactctg ctgcaatagt tctggtaaat cgccatcgct aacagcgaga
    34261 agaagacagg gccgccaatc atccacaatg tgctgtccca gtctccggct tcaaccacag
    34321 gttgaatgat ggtgaagacg ttcgcaaatg tcaccaccag aacgacaacc accgtcgcaa
    34381 tcattgccga catacgcgtt ttgaaaatca caaatggtct gtcgagatcc tgacgtgctt
    34441 taaagaatgg gaaagccagc gcgaggaaca ggtaaggaag cgtcatagac acgttcgcca
    34501 tcagcgtcag cttgttaaag aacgccgatg cggtaccgcc accaaacgaa accagcagga
    34561 tgaagacagt aaccaacccg cactgcatcc acatggcgat agacggcatc cccatcgcat
    34621 tcaggcgcgt catcggttcc ggccacaacg ctttcggcgt cccctggatg atggctttca
    34681 gcggtgaata gcaaagcgta aagaacgcac cggtataagc gaggaacatc gaaagtccgg
    34741 taatacgcgc aaaccatacg cccagcgaca atgacgcttc tggtgacaaa tgcagtgcgt
    34801 tacccagcgt caccccgagg ctcttcatca gcacataggt aatattgccg aggttaacgg
    34861 aaccattact taatacctgc tgccagtttg tgctgacgcc ccataaaaat attgccagcg
    34921 aataaccgat tgaaataaca atagcggcaa aaacaatacc tttggcaaag ttcttttctg
    34981 gattttccgt tttatcgacc agaccaccaa ccgcttcaat tccgccatag gcaaaaatag
    35041 caaataccac aaacgatagc attgccagac cggactgata acctgggttc ggtgatgcaa
    35101 ggaaattaat atcctgcgcg aaatgcccac catttaataa caaaatagta atgcttacta
    35161 acagcaatac taaattcaga cacattactg caataccgcc caccgcagta atgcgggcaa
    35221 ttttattaat cccctttgaa gcaacgacgg tgaccagaat catccatgcc actgccagca
    35281 gaccaaccac ctgcgtaggc tccagtccgg taatacgcca gtgctgggtc atgtcgctac
    35341 catagaggaa tgttgagaac ggaacccaaa ctttcgcgga ggtactcacc atccagatga
    35401 tataagagga aaaccacata aatgtaccaa tgaaggcaaa acgtggtccg acactattat
    35461 tcatccagga atagatacca ccttcttctt tgcgataagc agctcccatt tcagccatca
    35521 ttaaggcgaa tggaataaag aataacaatg cagaaaatat ataaaaggga atcgcactat
    35581 aacccattaa gtaataagcc gatgggctat tggcaaatcc aaatacggaa gtaaagatca
    35641 tcaatatgag tcctatcaga ctcatctttt ttatcgtgtg aggcatgaaa ccatccttca
    35701 tttcagcgcg tgtataaaac gcgcccactg aactgcactg cccaacattg agcgggcagc
    35761 gtaaaaacca atacagaatt gatacctgga tgatatcaaa tatccagtct taaattgtgg
    35821 cctttagcgg aaaaatgaaa acatatgcta cagaatggcg cttatcacgc cattcttcgc
    35881 ggttaattca gtaatttttc agaattatgc ccggtcaacg ctaaaggcga tgacttcagc
    35941 cagtgtctcc gcgcccagcg ccaacatcac cagacgatca acgcctaatg ccacgccgga
    36001 acagtcaggc ataccgactt tcaaggcttc aatcagattc tggtcaatgg ggtgctgcgg
    36061 caaaccgcgc gccgcgcgct tacggttatc ttgttcaaag cgttgttgct gctcacgggc
    36121 atccgtcaat tcatggaaac cattcgccag ctcaatacct ttataataaa cctcaaagcg
    36181 ttcagcgacc cgatgatctt cggtactgat ttgcgccagt gatgcctggc tggccggaaa
    36241 gtggtacaca aaggtcggtt tttctttgcc aatatttggc tctacgccaa aggtaaacag
    36301 cagttgtagc agcgtgtcgc ggtcttcttc ggtatcggca acgttgctca aatccagttt
    36361 cgctgccact tcccgcagtt gcgttttgtc agcagagagt gggtcaattt ccagataacg
    36421 caagaaagct tgttgataag aaaggctttc tgctgccggg cagtccagca cctgttgtaa
    36481 gagatcgtcc acctcgttca tcaaccggta catatcatag tgcggtcgat accactcaag
    36541 catagtgaac tcagggttgt gataacgccc catctcttca ttacggaagc tgcggcacag
    36601 ctggaatacc ggcccacaac cagcaaccag caggcgtttc atatggtatt ccgggctggt
    36661 cattaaccag agattcatcc cctgcgaatg cccggggcct acgaaacgtg tctcaaacgg
    36721 gaccaaatga atatcggtta ccgtcgcctg gctcatacaa ggcgtctcca cctccagcac
    36781 tccacgatcg gcaaagaaac gacggatctc cgccataatc gtcgcgcgtt ttaataagtt
    36841 aggaatggat gcgctcggct gccaggatgc cgtttcgctc atagttaaat ctccagtttt
    36901 tgacaagggc acgaagtcta ctcgcaacgc gacggcgaga caaattttac gcaggaatca
    36961 aacaagggtg ggcagtgact aaaaaaagca cgatctgatg gtttagtaat taaattaatc
    37021 atcttcagtg ataatttagc cctcttgcgc actaaaaaaa tcgatctcgt caaatttcag
    37081 acttatccat cagactatac tgttgtacct ataaaggagc agtggaatag cgttcgcaga
    37141 ccgtaacttt caggcactta ccctgaagta cgtggctgtg ggataaaaac aatctggagg
    37201 aatgtcgtgc aaacctttca agccgatctt gccattgtag gcgccggtgg cgcgggatta
    37261 cgtgctgcaa ttgctgccgc gcaggcaaat ccaaatgcaa aaatcgcact aatctcaaaa
    37321 gtatacccga tgcgtagcca taccgttgct gcagaagggg gctccgccgc tgtcgcgcag
    37381 gatcatgaca gcttcgaata tcactttcac gatacagtag cgggtggcga ctggttgtgt
    37441 gagcaggatg tcgtggatta tttcgtccac cactgcccaa ccgaaatgac ccaactggaa
    37501 ctgtgggggt gcccatggag ccgtcgcccg gatggtagcg tcaacgtacg tcgcttcggc
    37561 ggcatgaaaa tcgagcgtac ctggttcgcc gccgataaga ccggcttcca tatgctgcac
    37621 acgctgttcc agacctctct gcaattcccg cagatccagc gttttgacga acatttcgtg
    37681 ctggatattc tggttgatga tggtcatgtt cgcggcctgg tagcaatgaa catgatggaa
    37741 ggcacgctgg tgcagatccg tgctaacgcg gtcgttatgg ctaccggcgg tgcgggtcgc
    37801 gtttatcgtt acaacaccaa cggcggcatc gttaccggtg acggtatggg tatggcgcta
    37861 agccacggcg ttccgctgcg tgacatggaa ttcgttcagt atcacccaac cggtctgcca
    37921 ggttccggta tcctgatgac cgaaggctgc cgcggtgaag gcggtattct ggtcaacaaa
    37981 aatggctacc gttatctgca agattacggc atgggcccgg aaactccgct gggcgagccg
    38041 aaaaacaaat atatggaact gggtccacgc gacaaagttt ctcaggcctt ctggcacgaa
    38101 tggcgtaaag gcaacaccat ctccacgcct cgtggcgatg tggtttatct cgacctgcgt
    38161 cacctcggcg agaaaaaact gcatgaacgt ctgccgttca tctgcgaact ggcgaaagcg
    38221 tacgttggcg tcgatccggt taaagaaccg attccggtac gtccgaccgc acactacacc
    38281 atgggcggta tcgaaaccga tcagaactgt gaaacccgca ttaaaggtct gttcgccgtg
    38341 ggtgaatgtt cctctgttgg tctgcacggt gcaaaccgtc tgggctccaa ctccctggcg
    38401 gaactggtgg tcttcggccg tctggccggt gaacaagcga cagagcgtgc agcaactgcc
    38461 ggtaatggca acgaagcggc aattgaagcg caggcagctg gcgttgaaca acgtctgaaa
    38521 gatctggtta accaggatgg cggcgaaaac tgggcgaaga tccgcgacga aatgggcctg
    38581 gctatggaag aaggttgcgg tatctaccgt acgccggaac tgatgcagaa aaccatcgac
    38641 aagctggctg agctgcagga acgcttcaag cgcgtgcgca tcaccgacac ctccagcgtg
    38701 ttcaacaccg acctgctcta caccattgaa ctgggccacg gtctgaacgt tgctgaatgt
    38761 atggcgcact ccgcaatggc acgtaaagag tcccgcggcg cacaccagcg tctggacgaa
    38821 ggttgcaccg agcgtgacga cgtcaacttc ctcaaacaca ccctcgcctt ccgcgatgct
    38881 gatggcacga ctcgcctgga gtacagcgac gtgaagatta ctacgctgcc gccagctaaa
    38941 cgtgtttacg gtggcgaagc ggatgcagcc gataaggcgg aagcagccaa taagaaggag
    39001 aaggcgaatg gctgagatga aaaacctgaa aattgaggtg gtgcgctata acccggaagt
    39061 cgataccgca ccgcatagcg cattctatga agtgccttat gacgcaacta cctcattact
    39121 ggatgcgctg ggctacatca aagacaacct ggcaccggac ctgagctacc gctggtcctg
    39181 ccgtatggcg atttgtggtt cctgcggcat gatggttaac aacgtgccaa aactggcatg
    39241 taaaaccttc ctgcgtgatt acaccgacgg tatgaaggtt gaagcgttag ctaacttccc
    39301 gattgaacgc gatctggtgg tcgatatgac ccacttcatc gaaagtctgg aagcgatcaa
    39361 accgtacatc atcggcaact cccgcaccgc ggatcagggt actaacatcc agaccccggc
    39421 gcagatggcc aagtatcacc agttttccgg ttgcatcaac tgtggtctgt gctacgccgc
    39481 gtgcccgcag tttggcctga acccagagtt catcggtccg gctgccatta cgctggcgca
    39541 tcgttacaac gaagatagcc gcgaccacgg taagaaggag cgtatggcgc agttgaacag
    39601 ccagaacggc gtatggagct gtactttcgt gggctactgc tccgaagtct gcccgaaaca
    39661 cgtcgatccg gctgcggcca ttcagcaggg caaagtagaa agttcgaaag attttcttat
    39721 cgcgaccctg aaaccacgct aaggagtgca acatgacgac taaacgtaaa ccgtatgtac
    39781 ggccaatgac gtccacctgg tggaaaaaat tgccgtttta tcgcttttac atgctgcgcg
    39841 aaggcacggc ggttccggct gtgtggttca gcattgaact gattttcggg ctgtttgccc
    39901 tgaaaaatgg cccggaaagc tgggcgggat tcgtcgactt tttacaaaac ccggttattg
    39961 tgatcattaa cctgatcact ctggcggcag ctctgctgca caccaaaacc tggtttgaac
    40021 tggcaccgaa agcggccaat atcattgtaa aagacgaaaa aatgggacca gagccaatta
    40081 tcaaaagtct ctgggcggta actgtggttg ccaccatcgt aatcctgttt gttgccctgt
    40141 actggtaagg agcctgagat gattaatcca aatccaaagc gttctgacga accggtattc
    40201 tggggcctct tcggggccgg tggtatgtgg agcgcaatta ttgcgccggt gatgatcctg
    40261 ctggtgggta ttctgctgcc gctggggctg tttccgggtg atgcactgag ctacgagcgc
    40321 gttctggcgt tcgcgcagag cttcattggt cgcgtattcc tgttcctgat gatcgttctg
    40381 ccgctgtggt gtggtttaca ccgtatgcac cacgcgatgc acgatctgaa aatccacgta
    40441 cctgcgggca aatgggtttt ctacggtctg gctgctatcc tgacagttgt cacgctgatt
    40501 ggtgtcgtta caatctaacg catcgccaat gtaaatccgg cccgcctatg gcgggccgtt
    40561 ttgtatggaa accagaccct atgttcaaaa tgacgctctg cgccttatta attaccgcct
    40621 cttgctccac atttgctgcc cctcaacaaa tcaacgatat tgtgcatcgc acaataaccc
    40681 cgcttataga gcaacaaaag atccccggta tggcggtggc ggtaatttat cagggtaaac
    40741 cttattactt tacctggggc tatgcggaca tcgccaaaaa gcagcccgtc acacagcaaa
    40801 cgttgtttga gttaggctcg gtcagcaaaa catttacggg cgtacttggt ggcgacgcta
    40861 ttgcacgagg ggaaatcaag ttaagcgatc ccgcgacaaa atactggcct gaacttaccg
    40921 ctaaacaatg gaatgggatc acactattac atctcgcaac ctacaccgct ggcggcctgc
    40981 cattgcaggt gccggatgag gtgaaatcct caagcgactt gctgcgcttc tatcaaaact
    41041 ggcaacctgc atgggctcca ggaacacaac gtctgtatgc caactccagt atcggtttgt
    41101 tcggcgcact ggctgtgaag ccgtctggtt tgagttttga gcaggcgatg caaactcgtg
    41161 tcttccagcc actcaaactc actcatacgt ggattaatgt gccgtccgca gaagaaaaga
    41221 attacgcctg gggatatcgc gaaggtaagg cagtgcatgt ttcgccaggg gcgttagatg
    41281 ctgaagctta tggtgtgaag tcgaccattg aagatatggc ccgctgggtg caaagcaatt
    41341 taaaacccct tgatatcact gagaaaacgc ttcaacaagg gatacaactg gcacaatctc
    41401 gctactggca aaccggcgat atgtatcagg gcctgggctg ggaaatgctg gactggccgg
    41461 taaatcctga catcatcatt aacggcagtg acaataaaat tgcactggca gcacgccccg
    41521 taaaagcgat tacgccccca gctcctgcag tgcgcgcatc atgggtacat aaaacggggg
    41581 cgaccggcgg atttggtagc tatgttgcgt ttattccaga aaaagagctg ggtatcgtga
    41641 tgctggctaa caaaaactat cccaatccag cgagagtcgc cgccgcctgg cagattctca
    41701 acgctctaca gtaaaatttc atcgggtccg aattttcgga ccttttctgc gcttttcctt
    41761 gctgtcatct acacttagaa aaaaccagta aggaaacatt tatgcgcctg ctccctctcg
    41821 ttgccgcagc gacagctgca tttctggtgg ttgcctgtag ttctcccaca ccaccgcgtg
    41881 gcgtgaccgt ggtaaataat ttcgatacca aacgctatct gggtacatgg tatgagattg
    41941 cccgttttga tcaccgtttt gaacgcgggc tggagaaagt gaccgcgacg tatagcctgc
    42001 gggatgacgg cggcctgaat gtcattaata aaggctataa cccggacaga gagatgtggc
    42061 agcagagtga aggaaaagcc tactttactg gagatccgcg tcgcgcagcc ctgaaagtgt
    42121 cgttctttgg gccattctac ggcggttata atgtcatcgc gctcgaccgg gagtaccgcc
    42181 acgcgctggt ttgcggcccg gaccgcgact acctgtggat actctctcgc acgccaacca
    42241 tttctgacga agtgaaacag gagatgctgg caatcgcgac ccgggaaggg tttgatatca
    42301 gtaagtttat ttgggtacag caacccggta gttaatgagt gctgagtttc agaccaataa
    42361 tccccaatac gattaacgcc agactcgcca ggcgcatcgg gttagcggac tcgccgagca
    42421 gcacaatgcc ggtgatggcc gcgccgactg cgccaatacc cgtccacacg gcataagccg
    42481 tccctactgg taacgatttc atcgcccagg caagtagcgc catactgaca atcatcgccg
    42541 tcacagtaat aacacttggc gtcaaacgac taaagccgtg ggtatatttc aggccaacgg
    42601 cccataccac ttccagcaga ccagcaataa ctaagataat ccaggacata tcaggctccg
    42661 gagaattggg gccgtccccg gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg
    42721 gttgaacgga tattttgcca ggtggcaggg gaatttcaac tattaccttt gtgactatcg
    42781 caaaaaaaca aacggcacga cataactgtc gtaccgttat tgcttactgc tgcgctttcg
    42841 ttgctgcgcc agaaatcgcg ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg
    42901 tgttgcaggc agttaatact gttgaaagca ccagaacaga aaagatcgct gcaattgtct
    42961 tcttcaccat aacgtcttcc ttttaggtaa tgttatttat gtttgcctat agcaaattaa
    43021 taatagacaa catcaccaaa aatgatggaa atctgatgat ttttcggaag agaagaaaaa
    43081 ttagctggca gcgcgggaga tggaattacc gagatgttta atgtcttcac caaaaccgcg
    43141 agcggtatta cagcccgtga gcagcgcact ggcaagcaaa acaagaacga taaggcgttt
    43201 catcatctct ggcctgaata agtgatggtg cagcctgcag gccgcaccac aaccgcatta
    43261 cttcacgcga gagacgtatt caccagagcg ggtatccact ttgatgactt cgccgatctg
    43321 cacgaacagc ggaactttaa ccacagcgcc agtagacagg gtagccggtt tgccgccagt
    43381 acctgcggta tcacctttca ggcccggatc ggtatccacg atttccagtt caacgaagtt
    43441 cggcggagta acggagatcg gctgaccatt ccacagagtt acgatacact ctgcctgatc
    43501 cagcagccat ttcgcgttgt cgccaattgc ttttgcatca gcagacagct gctcgaaagt
    43561 ttcgttgttc atgaagtgcc agaactcacc gtcgttgtac aggtaagtca ggttcatatc
    43621 gacaacatca gcgccttcag cggaatcagt agatttgaag gttttttcta cgcgagtacc
    43681 ggtcagcaga cgacgcagtt taacgcgagc aaatgcctgg cctttacccg gttttacgaa
    43741 ttcactcgct tcaaccgcgt aaggttcgcc gtctaacatg attttaagac cagcacgaaa
    43801 atcgttgcta tagtacgttg ccataaggcc ctctgaaatt tgttaattgg tagctaagcc
    43861 acaaaatggc gcatattgta accctaaata ccccatccag agaagattgg ttaacgcaac
    43921 ttgccgatgt tgtgaccgat cctgatgaac ttctgcgtct tttgaatata gacgcggacg
    43981 aaaaactgtt ggccggacgc agcgccaaaa agctttttgc cctgcgtgtg ccccgctcat
    44041 ttatcgatcg catggagaaa ggcaatccgg acgatcctct tttgcgtcag gtacttacct
    44101 cgcaagatga gtttgtcgtc gcgcccggat tctccaccga cccactggaa gaacagcaca
    44161 gcgtagtgcc tggtttgttg cataaatacc acaaccgggc gcttttgctg gtcaaaggcg
    44221 gctgcgcggt aaattgccgc tattgcttcc gtcgccactt cccctatgcc gaaaatcagg
    44281 gcaacaagcg taactggcaa actgcacttg agtatgttgc tgcgcatccg gaactggacg
    44341 agatgatttt ctccggcggc gatccgctga tggcgaaaga tcacgagctg gactggttgc
    44401 tcacacaact ggaagccatc ccgcatatca aacgtctgcg gattcacagc cgtctgccga
    44461 ttgtgatccc ggcacgcatc acagatgcgc tggttgaacg cttttcacat tctacgctgc
    44521 aaatcttgct ggtgaatcac atcaaccatg ccaatgagat agatgaaaca ttccgtcagg
    44581 cgatggctaa gttgcgccgg gtaggcgtta ctttgctcaa ccagagcgtt ctgttacgtg
    44641 gcgtgaacga taacgcacaa acgctggcaa acctgagtaa tgcgttgttc gatgccggcg
    44701 tgatgccgta ttacctgcat gtgctcgata aggtacaggg cgcggcgcat tttatggtga
    44761 gtgatgacga agcacggcag attatgcgtg agttgctgac actggtgtcg ggatatctgg
    44821 tgcctaaact ggcgcgagaa attggcggcg aacccagcaa aacgccgctg gatctccagc
    44881 tacgccagca gtaactgatt aaacagggaa gtttaatctt tgcacgttgc ataaccttta
    44941 attattcata tacattgctg ttattacttt ttatataacc aacaccaata attaaccttt
    45001 cttttctgtt tatatagagt gagcacggct atttatgtta ttttaacaac tattactacc
    45061 aagcccattt atctggctta taaattaaaa ttaattattt aattgagaaa tggttaggga
    45121 gaacctacat ggccataagc atcaagggtg ttaataccgg tgttattcgc aagagtaata
    45181 atttcattgc actggcgctg aaaattaaag agcctcgcaa taaagaatcg ttgttctttc
    45241 tgtctgtcat ggaactgcgc gaccttctga ttgcgctgga aagccgtctg catcaaaagc
    45301 ataaactgga tgctgctgca cgccttcagt atgagcaagc tcgcgataaa gtcattaaga
    45361 aaatggcaga aaatatccca gaaattctgg ttgatgagct taaaaatgcc gatattaatc
    45421 gtcgggttaa cacgctggaa cttaccgata atcagggtga aaatcttacc ttcgtattaa
    45481 cgctacatga cggtagcaaa tgtgagttag tcatcaatga attgcagatt gaaatgctgg
    45541 cgcgagcaat cattcatgca attaataatg cggaaatgcg tgaactggca ttacgtatta
    45601 cctcactgtt agatttcctg ccgttatacg acgttgactg ccaggacaat ggcaatctgg
    45661 aatatgacac ctattcccaa ccggaatgga aacataactt gttcaaccat tatctggcgg
    45721 tgctttaccg ctttaaagat gaaagtggca aagaacaatt tagcggcgca gtggtgaaaa
    45781 cgcgtgaagc aacgccgggt aaagaagtgg aggcgatcac tcgccgaatg cttgatttca
    45841 gcccccgact gaaaaaactc gcgggtgtgc catgtcaggt ctatgttcgc accgtagcgg
    45901 caaataacgc ccaaccgcta acccaggatc aatgcttacg cgcgctgcat cacttacgtg
    45961 ttcaatccac cagcaaaact gcaccacagg cgaaataatc ccatctgttt tactacgagg
    46021 ctggaagtat ccagcctctg gatcccatca gttcgggcac ttatatacct ggccgataat
    46081 ctggctatcc gtcgggacaa aactggacaa catcccctgc gacgggctgc tgatgccata
    46141 aatcacgtta ccgcccattg cagccgcctg gttgcgcaga tcgtttgctg cgccgcgcat
    46201 agaaccgccc tcttcaccgt gttgcccgga aagccagttg ctttgcttac ctgtcgcagt
    46261 accaatcagc tggcactctg cgcctggctg ctcgtccaca atgcgtacac tctgaccggc
    46321 agcactcaat tcgttgctgg agctacaacc cgccatcagt agcgcggcac cgacaatccc
    46381 tgctaagtat tttacgtgca tgttattccc cataatcaat gagctggacg ccactcgcgt
    46441 cgtccgtgtc tgaatcttat actagaaaga tgaccaaaag aaaaaccccc agacatttct
    46501 gcccgggggt ttgtttattt ctgcgaggtg cagggcaatt acatcatgcc gcccatgcca
    46561 cccatgccgc ccataccgcc agcagcgcct aagtcagctg catcgttttt cggcaggtcg
    46621 gtaaccatgc actcggtggt gatcatcagg ccagccacag aagccgcgta ctgcagagca
    46681 gaacgggtta ctttggttgg atccaggata cccatgtcga tcatgttgcc gtattcttcg
    46741 gttgctgcgt tgtaaccgta gttaccgtcg ccgcctttaa cggtgttagc aacaacagac
    46801 ggttcttcgc cgcagttcag gacgatctga cgcagcggag cttccattgc acgcagtgca
    46861 actttgatac ccacgttctg gtcttcgttc tgaccacgca ggtcagccag tttagacgct
    46921 acgcggatca gcgcaacacc accaccagca accacgcctt cttctaccgc agcacgggtc
    46981 gcgtgcaggg cgtcttcaac gcgtgctttt ttctctttca tttcaacttc ggtagcagca
    47041 cctactttga taactgcaac gccgcctgcc agtttcgcta cgcgctcctg cagtttttca
    47101 cggtcgtagt cagaagttgc ttcttcaatc tgctgacgga tctgagcaac acggccctgg
    47161 attgcagctt cttcacccac gccatcgatg atggtggtag tgtctttgtt gatcacaaca
    47221 cgtttagcct gacccaggtc ttccagggtt gctttttcca gctccatacc gatctcttca
    47281 gagattacgg taccgccagt cagggttgcg atatcctgca gcatagcttt acgacgatcg
    47341 ccgaagcccg gagctttaac cgcagcaact ttcacgatgc cacgcatggt gttaacaacc
    47401 agagttgcca gcgcttcgcc ttctacatct tcagcgatga tcagcagcgg tttgcctgct
    47461 ttggcaacag cttccagaac cggcagcatt tcgcggatgt tggagatttt cttgtcagcc
    47521 agcaggatga acgggctttc cagttctact gcgccagttt ccggcttgtt gatgaagtaa
    47581 ggagacaggt agccacggtc gaactgcata ccttcaacca cgtccagttc gtcctgcaga
    47641 ccggtaccgt cttcaacggt gataacgcct tctttaccga ctttgtccat cgcttcagcg
    47701 atcagtttac ctacggtttc gtcggagtta gcggagatgg taccaacctg agcaatcgct
    47761 ttagagtcag agcacggtac ggacagcgct ttcagttctt caactgcagc ggtaacagct
    47821 ttgtcgatac cacgtttcag gtccatcggg ttcatgcccg cagcaacagc tttcagacct
    47881 tcagtgatga tagcctgagc cagtacggtt gcagtggtgg taccgtcgcc tgcagcgtcg
    47941 ttcgctttag aggcaacttc tttcaccatc tgcgcaccca tgttttcgaa cttatcttcc
    48001 agttcgattt cacgagcaac ggaaacacca tctttggtga tggtcggtgc accgaaagat
    48061 ttatccagaa ctacgttacg gcctttcgga ccgagggtaa ctttcactgc atctgccagt
    48121 acgtttacgc cgcgcagcat tttcacacga gcgtcgttac cgaattttac gtctttagct
    48181 gccattatct ttattcctta aattcgtatg ttcagtgtcg tgcgcggatt acgcttcaac
    48241 aattgccaga atgtcgcttt cggacatgat caacacttct tcattgtcga tcttctcaga
    48301 tttcacaccg tagccatcgt tgaaaataac gatgtcgcca actttcacat ccagcggctt
    48361 cacttcgcca ttttcaagga tacggccatt gccgacagcc agcacttcgc cgcgggtgga
    48421 tttagccgct gcagagccgg tcagaacgat gccgccagca gatttagttt caacttcttt
    48481 acgcttgacg atcacgcgat catgcaatgg acgaatattc attgataact ctcctttgag
    48541 aaagtccgta tctgttatgg gtgacgccgg agcttacgtg gtttcccggc tggtgaccag
    48601 agaaatgggg atgaggcttc gccccttcaa gggggaaaaa gaaaaaaaat tctgatcagc
    48661 acaaaatcgg gtgaaaaccc tgattcacct cacatttcat cgcaatttct tcatcaccgt
    48721 cataagcgaa tctgattgtg ctaccatcga aaatctacgc agttgcccat aatttgggcg
    48781 caatcggaca tcaacccatg agtggactca aacaagaact gggactggcc cagggcattg
    48841 gcctgctatc gacgtcatta ttaggcactg gcgtgtttgc cgttcctgcg ttagctgcgc
    48901 tggtagcggg caataacagc ctgtgggcgt ggcccgtttt gattatctta gtgttcccga
    48961 ttgcgattgt gtttgcgatt ctgggtcgcc actatcccag cgcaggcggc gtcgcgcact
    49021 tcgtcggtat ggcgtttggt tcgcggcttg agcgagtcac cggctggctg tttttatcag
    49081 tcattcccgt gggtttgcct gccgcactac aaattgcagc cggattcggc caggcaatgt
    49141 ttggctggca tagctggcaa ctgttgttgg cagaactcgg tacgctggcg ctggtgtggt
    49201 atatcggtac tcgcggtgcc agttccagtg ctaatctaca aacagttatt gccggactta
    49261 tcgtcgcgct gattgtcgct atctggtggg cgggcgatat caaacctgcg aatatcccct
    49321 tccctgcacc tggtaatatc gaacttaccg ggttatttgc tgcgttatca gtgatgttct
    49381 ggtgctttgt cggtctggaa gcatttgccc atcttgcctc ggaatttaaa aatccagagc
    49441 gtgattttcc tcgtgctttg atgattggtc tgctgctggc aggattagtc tactggggct
    49501 gtacggtagt cgtcttacac ttcgacgcct atggtgaaca aatggcggca gcagcatcgc
    49561 ttccaaaaat tgtagtgcaa ctgttcggtg taggagcgtt atggattgcc tgcgtgattg
    49621 gctatctggc ctgctttgcc agtctcaaca tttatataca gagcttcgcc cgcctggtct
    49681 ggtcgcaggc gcaacataat cctgaccact acctggcacg cctctcttct cgtcatatcc
    49741 cgaataatgc cctcaatgcg gtgctcggct gctgcgtggt gagcacgttg gtgattcatg
    49801 ctttagggat caatctggac gcacttatca tctatgccaa tggcatcttt attatgattt
    49861 atctgttatg catgctggca ggctgtaaat tattgcaagg acgttatcga ctactggcgg
    49921 tggttggcgg gctgttatgc gttctgttac tggcaatggt cggctggaaa agtctctatg
    49981 cgctgatcat gctggcgggg ttatggctgt ttctgccaaa acgaaaaacg ccggaaaatg
    50041 gcataaccac ataatccggc gtttcggcat taatcctggc gatcgtcttt atgatcaagg
    50101 cggtcgcggt catcatcctt tcgctggtac tcaccatcaa aagtattacc gccaccggtc
    50161 ccggcgctaa aaccgccgcc tggcatgcga gaaaagcgca aatgcggcat caacttcact
    50221 gtcagatgct tttgcaccgg cggtaataaa agcagaagac cgaggaagtc ggtgaaaaag
    50281 cccggtaata aaagcagcaa accggcaatg atcagcgaaa cacttttaat catctccgcc
    50341 gctgggtttt cacccgccgc cattttttgc tgcatcagca cgaaattctt aaagccctgg
    50401 ttacgtacca gtgacatacc gataactgac gtgaatataa ccagcacgag ggtcagcaat
    50461 acccccaata catgggcaac ctgaataaag attgaaatct caatatagac ataaaggaaa
    50521 atggcaataa aaggtaacca gcgcaaaggt ttctcctgta atagcagccg gttaaccccg
    50581 gctacctgaa tgggttgcga atcgcgttta gcttatattg tggtcattag caaaatttca
    50641 agatgtttgc gcaactattt ttggtagtaa tcccaaagcg gtgatctatt tcacaaatta
    50701 ataattaagg ggtaaaaacc gacacttaaa gtgatccaga ttacggtaga aatcctcaag
    50761 cagcatatga tctcgggtat tcggtcgatg caggggataa tcgtcggtcg aaaaacattc
    50821 gaaaccacat atattctgtg tgtttaaagc aaatcattgg cagcttgaaa aagaaggttc
    50881 acatgtcaaa caacattcgt atcgaagaag atctgttggg taccagggaa gttccagctg
    50941 atgcctacta tggtgttcac actctgagag cgattgaaaa cttctatatc agcaacaaca
    51001 aaatcagtga tattcctgaa tttgttcgcg gtatggtaat ggttaaaaaa gccgcagcta
    51061 tggcaaacaa agagctgcaa accattccta aaagtgtagc gaatgccatc attgccgcat
    51121 gtgatgaagt cctgaacaac ggaaaatgca tggatcagtt cccggtagac gtctaccagg
    51181 gcggcgcagg tacttccgta aacatgaaca ccaacgaagt gctggccaat atcggtctgg
    51241 aactgatggg tcaccagaaa ggtgaatatc agtacctgaa cccgaacgac catgttaaca
    51301 aatgtcagtc cactaacgac gcctacccga ccggtttccg tatcgcagtt tactcttccc
    51361 tgattaagct ggtagatgcg attaaccaac tgcgtgaagg ctttgaacgt aaagctgtcg
    51421 aattccagga catcctgaaa atgggtcgta cccagctgca ggacgcagta ccgatgaccc
    51481 tcggtcagga attccgcgct ttcagcatcc tgctgaaaga agaagtgaaa aatatccaac
    51541 gtaccgctga actgctgctg gaagttaacc ttggcgcaac agcaatcggt actggtctga
    51601 acacgccgaa agagtactct ccgctggcag tgaaaaaact ggctgaagtc actggcttcc
    51661 catgcgtacc ggctgaagac ctgatcgaag cgacctctga ctgcggcgct tatgttatgg
    51721 ttcacggcgc gctgaaacgc ctggctgtga agatgtccaa aatctgtaac gacctgcgct
    51781 tgctctcttc tggcccacgt gccggcctga acgagatcaa cctgccggaa ctgcaggcgg
    51841 gctcttccat catgccagct aaagtaaacc cggttgttcc ggaagtggtt aaccaggtat
    51901 gcttcaaagt catcggtaac gacaccactg ttaccatggc agcagaagca ggtcagctgc
    51961 agttgaacgt tatggagccg gtcattggcc aggccatgtt tgaatccgtt cacattctga
    52021 ccaacgcttg ctacaacctg ctggaaaaat gcattaacgg catcactgct aacaaagaag
    52081 tgtgcgaagg ttacgtttac aactctatcg gtatcgttac ttacctgaac ccgttcatcg
    52141 gtcaccacaa cggtgacatc gtgggtaaaa tctgtgccga aaccggtaag agtgtacgtg
    52201 aagtcgttct ggaacgcggt ctgttgactg aagcggaact tgacgatatt ttctccgtac
    52261 agaatctgat gcacccggct tacaaagcaa aacgctatac tgatgaaagc gaacagtaat
    52321 cgtacagggt agtacaaata aaaaaggcac gtcagatgac gtgccttttt tcttgtgagc
    52381 agtaacttaa aaataacaat ctaatatcaa cttgttaaaa aacaaggaag gctaatatgc
    52441 tagttgtaga actcatcata gttttgctgg cgatcttctt gggcgccaga ttggggggaa
    52501 taggtattgg ttttgcaggc ggattggggg tgctggttct tgccgctatt ggcgttaaac
    52561 ccggtaacat cccgttcgat gtcatttcca ttatcatggc ggttatcgcc gctatttctg
    52621 ccatgcaggt tgctggcggt ctggactatc tggttcatca gacagaaaag ctgctgcgcc
    52681 gtaacccgaa atacatcacg atcctcgcac cgatcgtgac ctatttcctg acaatctttg
    52741 ctggtactgg caacatctct ctggcgacac tgccagttat cgctgaagtt gcgaaggaac
    52801 aaggcgttaa accttgccgt ccgctgtcta ctgcagtggt atccgcgcag attgcgatca
    52861 ccgcatcgcc aatctcagcg gcagtggttt acatgtcttc cgtaatggaa ggtcatggca
    52921 tcagctacct ccatctgctc tccgtggtca tcccgtccac cctgctggcg gttctggtga
    52981 tgtccttcct ggtcactatg ctgttcaact ccaaactctc tgacgatccg atttatcgca
    53041 agcgtctgga agagggcctg gttgaactgc gcggtgaaaa gcagattgaa atcaaatccg
    53101 gtgcaaaaac gtccgtctgg ctgttcctgc tgggcgtagt tggcgttgtt atctatgcaa
    53161 tcatcaacag cccaagcatg ggtctggttg aaaaaccgct gatgaacacc accaacgcaa
    53221 tcctgatcat catgctcagc gttgcaactc tgaccaccgt tatctgtaaa gtcgataccg
    53281 acaacatcct caactccagc accttcaaag caggtatgag cgcctgtatt tgtatcctgg
    53341 gtgttgcatg gctgggcgat actttcgttt ccaacaacat cgactggatc aaagataccg
    53401 ctggtgaagt gattcagggt catccgtggc tgctggccgt catcttcttc tttgcgtctg
    53461 ctctgctgta ctctcaggct gcaaccgcaa aagcactgat gccgatggct ctggcactga
    53521 acgtttctcc gctgaccgct gttgcttctt tcgctgcggt gtctggtctg ttcattctgc
    53581 cgacctaccc gacgctggtt gctgcggtac agatggatga cacgggtact acccgtatcg
    53641 gtaaattcgt cttcaaccat ccgttcttca tcccgggtac tctgggtgtt gccctggccg
    53701 tttgcttcgg cttcgtgctg ggtagcttca tgctgtaatg accaatcgcg gggcttcagg
    53761 ccccgctttc tttcccgccg actaacatcc ttcccccgtc cgttgtatag tgacctctct
    53821 cttgcggttc catctgttct tgcgaggtgt ttatgcttga tgaaaaaagt tcgaataccg
    53881 cgtctgtcgt ggtgctatgt acggcaccgg atgaagcgac agcccaggat ttagccgcca
    53941 aagtgctggc ggaaaaactg gcggcctgcg cgaccttgat ccccggcgct acctctctct
    54001 attactggga aggtaagctg gagcaagaat acgaagtgca gatgatttta aaaactaccg
    54061 tatctcacca gcaggcactg ctggaatgcc tgaagtctca tcatccatat caaaccccgg
    54121 aacttctggt tttacctgtt acacacggag acacagatta cctctcatgg ctcaacgcat
    54181 ctttacgctg atcctgctac tttgcagcac ttccgttttt gccggattat tcgacgcgcc
    54241 gggacgttca caatttgtcc tcgcggatca agcctttgct tttgattttc agcaaaacca
    54301 acatgacctg aatctgacct ggcagatcaa agacggttac tacctctacc gtaaacaggt
    54361 ccgcattacg ccggaacacg cgaaaattgc cgacgtgcag ctgccgcaag gcgtctggca
    54421 tgaagatgag ttttacggca aaagcgagat ttaccgcgat cggctgacgc ttcccgtaac
    54481 catcaaccag gcgagtgcgg gagcgacgtt aactgtcacc taccagggct gtgctgatgc
    54541 cggtttctgt tatccgccag aaaccaaaac cgttccgtta agcgaagtgg tcgccaacaa
    54601 cgcagcgtca cagcctgtgt ctgttccgca gcaagagcag cccaccgcgc aattgccctt
    54661 ttccgcgctc tgggcgttgt tgatcggtat tggtatcgcc tttacgccat gcgtgctgcc
    54721 aatgtaccca ctgatttctg gcatcgtgct gggcggtaaa cagcggcttt ccactgccag
    54781 agcattgttg ctgaccttta tttatgtgca ggggatggcg ctgacttaca cggcactggg
    54841 tctggtggtt gccgccgcag ggttacagtt ccaggcggcg ctacagcacc catacgtgct
    54901 cattggcctc gctatcgtct ttaccttgct ggcgatgtca atgtttggct tgtttaccct
    54961 gcaactcccc tcttcgctgc aaacgcgcct cacgctgatg agcaatcgcc aacagggcgg
    55021 ctcacctggc ggtgtgtttg ttatgggggc gattgccgga ctgatctgtt caccatgcac
    55081 caccgcaccg cttagcgcga ttctgctgta tatcgcccaa agcgggaaca tgttgctggg
    55141 gggcggcacg ctttatctct atgcgttggg catgggcctg ccgctgatgc taataaccgt
    55201 ctttggtaac cgcctgctgc cgaaaagcgg cccgtggatg gaacaagtca aaaccgcgtt
    55261 tggttttgtg atcctcgcac tgccggtctt cctgctggag cgagtgattg gtgatatatg
    55321 gggattacgc ttgtggtcgg cgcttggtgt cgcattcttt ggctgggcct ttatcaccag
    55381 cctacaggcc aaacgcggct ggatgcgcgt ggtgcaaata atcctgctgg cagcggcatt
    55441 ggttagcgtg cgcccacttc aggattgggc atttggtgcg acgcataccg cgcaaactca
    55501 gacgcatctc aactttacac aaatcaaaac ggtagatgag ttaaatcagg cgctcgttga
    55561 agccaaaggc aaaccggtga tgttagatct ctatgccgac tggtgcgtcg cctgtaaaga
    55621 gtttgagaaa tacaccttca gcgacccgca ggtgcaaaaa gcgttagcag acacggtatt
    55681 acttcaggcc aacgtcactg ccaacgacgc acaagatgtg gcgctgttaa agcatcttaa
    55741 tgtccttggc ctaccgacaa ttctcttttt tgacggacaa ggccaggagc atccacaagc
    55801 acgcgtcacg ggctttatgg atgctgaaac cttcagcgca catttgcgcg atcgccaacc
    55861 gtgaacgaca cttgcagtgg gaaagacgga ggaaaatagc gtgcaacgtg aagatgtact
    55921 gggagaagcc ctgaaattat tagaattaca agggattgcc aacaccacgc tggagatggt
    55981 tgctgaacgt gtggattatc cactggacga gctacgccgc ttctggccag acaaagaggc
    56041 aatcctctac gatgcgctgc gttatcttag ccaacagata gatgtctggc gtcgtcagct
    56101 gatgctggac gaaacgcaaa ccgccgaaca aaagttgctg gcacgttatc aggcgttatc
    56161 ggagtgcgtt aaaaacaacc gctatccggg ctgtctgttt atcgctgcct gtacgtttta
    56221 tcccgatcct ggccacccta ttcatcaact ggccgatcag caaaaaagcg cggcctacga
    56281 tttcacccac gaactattaa ccacactgga agttgacgat ccggcgatgg ttgcaaagca
    56341 gatggaactg gtgctggaag gctgtttaag ccgaatgctg gtgaatcgta gccaggcgga
    56401 tgtcgacacc gcacatcggc tggcggaaga tatcctgcgc ttcgcccgct gccgccaggg
    56461 tggtgctttg acctgacaga aacacagaaa agaagcgatt tgccgcaatc ttaagcagtt
    56521 gaatcgcttt tactgaaatt aggttgacga gatgtgcaga ttacggttta atgcgccccg
    56581 ttgcccggat agctcagtcg gtagagcagg ggattgaaaa tccccgtgtc cttggttcga
    56641 ttccgagtcc gggcaccaaa ttcatatcaa cggacctcca cggaggtccg tttttcgttt
    56701 caggacgcca caatttaagc gttctgccta aaaatcattt ctaccgaact caaccagatt
    56761 ctccccacat caccagcaat ttgcgggcat atcccaattc ggggaaatgt gttcctgagc
    56821 gatagcactg actgacgtga aatgtcgcgc ggccccgtga tgctgttgaa cgtcagatga
    56881 cgtcatcagg agcgtaacgc atccataaag cacaacctcg ggcagaacgc cgactgatga
    56941 gattttctga atgagaacaa agagaaatgt atcagtccgc ttgctcatgc aaagactaac
    57001 aatccataaa aatagtaagc gcgccgggca atttcccatg gattatttta tgaacatttt
    57061 tctttggcaa agatgatgaa ttttgatgat aaggaatatt acttctggtt ctcagtaaaa
    57121 tcctttcgta ataccatgta atcaagaagt ttatggctgg taaaaataac gtcttgcatt
    57181 caccaataat atgtaaataa aaccatctat agatggtaaa aataggttgt ggcaattatc
    57241 attgcatcat tcccttttcg aatgagtttc tattatgcaa caacctgtag ttcgcgttgg
    57301 cgaatggctt gttactccgt ccataaacca aattagccgc aatgggcgtc aacttaccct
    57361 tgagccgaga ttaatcgatc ttctggtttt ctttgctcaa cacagtggcg aagtacttag
    57421 cagggatgaa cttatcgata atgtctggaa gagaagtatt gtcaccaatc acgttgtgac
    57481 gcagagtatc tcagaactac gtaagtcatt aaaagataat gatgaagata gtcctgtcta
    57541 tatcgctact gtaccaaagc gcggctataa attaatggtg ccggttatct ggtacagcga
    57601 agaagaggga gaggaaataa tgctatcttc gcctccccct ataccagagg cggttcctgc
    57661 cacagattct ccctcccaca gtcttaacat tcaaaacacc gcaacgccac ctgaacaatc
    57721 cccagttaaa agcaaacgat tcactacctt ttgggtatgg ttttttttcc tgttgtcgtt
    57781 aggtatctgt gtagcactgg tagcgttttc aagtcttgat acacgtcttc cgatgagcaa
    57841 atcgcgtatt ttgctcaatc cacgcgatat tgacattaat atggtaaata aaagttgtaa
    57901 cagctggagt tccccgtatc agctctctta cgcgataggc gtgggtgatt tggtggcgac
    57961 atcacttaac accttctcca cctttatggt gcatgacaaa atcaactaca acattgatga
    58021 accgagcagt tccggtaaaa cattatctat tgcgtttgtt aatcagcgcc aataccgtgc
    58081 tcaacaatgc tttatgtcgg taaaattggt agacaatgca gatggttcaa ccatgctgga
    58141 taaacgttat gtcatcacta acggtaatca gctggcgatt caaaatgatt tactggagag
    58201 tttatcaaaa gcgttaaacc aaccgtggcc acaacgaatg caggagatgc tccagcaaat
    58261 tttgcctcat cgtggtgcgt tattaactaa tttttatcag gcacatgatt atttactgca
    58321 tggcgatgat aaatcattga accgtgccag tgaattattg ggtgagattg ttcaatcatc
    58381 cccagaattt acctacgcga gagcagaaaa agcattagtt gatatcgtgc gccattctca
    58441 acatccttta gatgaaaaac aattagcagc actgaataca gaaatagata acattgttac
    58501 actgccggaa ttgaataacc tgtccattat atatcaaata aaagcggtca gtgctctggt
    58561 aaaaggtaaa acagatgagt cttaccaggc gataaatact ggcattgatc ttgaaatgtc
    58621 ctggctaaat tatgtgttgc ttggcaaggt ttatgaaatg aaggggatga accgggaagc
    58681 agctgatgca tatctcaccg cttttaattt acgcccaggg gcaaacaccc tttactggat
    58741 tgaaaatggt atattccaga cttctgttcc ttatgttgta ccttatctcg acaaatttct
    58801 cgcttcagaa taagtgactc ccgggttgat tcatactcgg gaatatttgt tgttgatt
//
LOCUS       DACBVI010000012        57596 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.012, whole genome shotgun sequence.
ACCESSION   DACBVI010000012 DACBVI010000000
VERSION     DACBVI010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.012"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            97..1332
                     /gene="moeA"
                     /locus_tag="IU325_03805"
     CDS             97..1332
                     /gene="moeA"
                     /locus_tag="IU325_03805"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HAP5808163.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISADAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASPLKKSPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERERGN
                     VEAGEWVEVEPFNALFGGL"
     gene            1332..2081
                     /gene="moeB"
                     /locus_tag="IU325_03810"
     CDS             1332..2081
                     /gene="moeB"
                     /locus_tag="IU325_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HAP5808164.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCLFREMKLMRNPGCEVCGQ"
     gene            complement(2168..2830)
                     /gene="fsa"
                     /locus_tag="IU325_03815"
     CDS             complement(2168..2830)
                     /gene="fsa"
                     /locus_tag="IU325_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP5808165.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     ELHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            2961..3860
                     /locus_tag="IU325_03820"
     CDS             2961..3860
                     /locus_tag="IU325_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HAP5808166.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMKTVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYITPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            3866..6298
                     /locus_tag="IU325_03825"
     CDS             3866..6298
                     /locus_tag="IU325_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HAP5808167.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            6444..7259
                     /gene="ybiV"
                     /locus_tag="IU325_03830"
     CDS             6444..7259
                     /gene="ybiV"
                     /locus_tag="IU325_03830"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HAP5808168.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHVALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            7411..8676
                     /locus_tag="IU325_03835"
     CDS             7411..8676
                     /locus_tag="IU325_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 family protein"
                     /protein_id="HAP5808169.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQRIQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(8643..9746)
                     /locus_tag="IU325_03840"
     CDS             complement(8643..9746)
                     /locus_tag="IU325_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP5808170.1"
                     /translation="MDKKLKIADIAARTGLSPSTVSRVLAGKANTSARAREQVLACAR
                     ELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVIQSISKALSPHEVRLRYCAL
                     EEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADLAKPCVLINCRDERMRLPAI
                     APDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFAGIKESWRQHNLAFHHDRHL
                     LNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMAAGTVNALHRRGLRVPQDVS
                     VMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRLIRPDAPVGSLLLHGRLAVR
                     ESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            10041..11342
                     /locus_tag="IU325_03845"
     CDS             10041..11342
                     /locus_tag="IU325_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5808171.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSNLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWVHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHNPDKHPNLSKAELDFIRQ
                     DNEAPPIKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            11357..13720
                     /locus_tag="IU325_03850"
     CDS             11357..13720
                     /locus_tag="IU325_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP5808172.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTLIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLDFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPVEEKRTLLVNGVEGSEWMR"
     gene            complement(13900..15495)
                     /locus_tag="IU325_03855"
     CDS             complement(13900..15495)
                     /locus_tag="IU325_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HAP5808173.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIDG"
     gene            15714..16634
                     /gene="ldtB"
                     /locus_tag="IU325_03860"
     CDS             15714..16634
                     /gene="ldtB"
                     /locus_tag="IU325_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAP5808174.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(16692..17810)
                     /locus_tag="IU325_03865"
     CDS             complement(16692..17810)
                     /locus_tag="IU325_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HAP5808175.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     LKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLFVMLPAW"
     gene            complement(17807..18274)
                     /locus_tag="IU325_03870"
     CDS             complement(17807..18274)
                     /locus_tag="IU325_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MntR"
                     /protein_id="HAP5808176.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            18460..18588
                     /gene="mntS"
                     /locus_tag="IU325_03875"
     CDS             18460..18588
                     /gene="mntS"
                     /locus_tag="IU325_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HAP5808177.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            18860..20443
                     /locus_tag="IU325_03880"
     CDS             18860..20443
                     /locus_tag="IU325_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP5808178.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAMLLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGLIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPMQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(20492..21007)
                     /gene="ompX"
                     /locus_tag="IU325_03885"
     CDS             complement(20492..21007)
                     /gene="ompX"
                     /locus_tag="IU325_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HAP5808179.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(21060..21125)
                     /locus_tag="IU325_03890"
     CDS             complement(21060..21125)
                     /locus_tag="IU325_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808180.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            21360..22247
                     /gene="rhtA"
                     /locus_tag="IU325_03895"
     CDS             21360..22247
                     /gene="rhtA"
                     /locus_tag="IU325_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HAP5808181.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLIGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            22546..23049
                     /gene="dps"
                     /locus_tag="IU325_03900"
                     /gene_synonym="pexB"
     CDS             22546..23049
                     /gene="dps"
                     /locus_tag="IU325_03900"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HAP5808182.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            23453..24199
                     /gene="glnH"
                     /locus_tag="IU325_03905"
     CDS             23453..24199
                     /gene="glnH"
                     /locus_tag="IU325_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HAP5808183.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            24338..24997
                     /gene="glnP"
                     /locus_tag="IU325_03910"
     CDS             24338..24997
                     /gene="glnP"
                     /locus_tag="IU325_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HAP5808184.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            24994..25716
                     /gene="glnQ"
                     /locus_tag="IU325_03915"
     CDS             24994..25716
                     /gene="glnQ"
                     /locus_tag="IU325_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017491583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HAP5808185.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            25833..28058
                     /gene="ybiO"
                     /locus_tag="IU325_03920"
     CDS             25833..28058
                     /gene="ybiO"
                     /locus_tag="IU325_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HAP5808186.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLSAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTIWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNDAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVTELMENEDIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAAPLPPGEPT
                     L"
     gene            complement(28055..28981)
                     /gene="rlmF"
                     /locus_tag="IU325_03925"
     CDS             complement(28055..28981)
                     /gene="rlmF"
                     /locus_tag="IU325_03925"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HAP5808187.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSVQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            29257..29517
                     /locus_tag="IU325_03930"
     CDS             29257..29517
                     /locus_tag="IU325_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAP5808188.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            29782..32064
                     /locus_tag="IU325_03935"
     CDS             29782..32064
                     /locus_tag="IU325_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HAP5808189.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAARLNVMGE
                     KTHDAGRDKVKNERYGIAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTASIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            32106..32783
                     /gene="ybiX"
                     /locus_tag="IU325_03940"
     CDS             32106..32783
                     /gene="ybiX"
                     /locus_tag="IU325_03940"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HAP5808190.1"
                     /translation="MMYHIPGVLSPKDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            32857..33123
                     /gene="ybiI"
                     /locus_tag="IU325_03945"
     CDS             32857..33123
                     /gene="ybiI"
                     /locus_tag="IU325_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HAP5808191.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            33388..33648
                     /locus_tag="IU325_03950"
     CDS             33388..33648
                     /locus_tag="IU325_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAP5808192.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(33788..34873)
                     /locus_tag="IU325_03955"
     CDS             complement(33788..34873)
                     /locus_tag="IU325_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HAP5808193.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGMPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQKTLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARKVGMPEETLQAFCQQLAS"
     gene            complement(35014..35976)
                     /gene="ybiB"
                     /locus_tag="IU325_03960"
     CDS             complement(35014..35976)
                     /gene="ybiB"
                     /locus_tag="IU325_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HAP5808194.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPADKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            complement(36004..38154)
                     /gene="dinG"
                     /locus_tag="IU325_03965"
     CDS             complement(36004..38154)
                     /gene="dinG"
                     /locus_tag="IU325_03965"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HAP5808195.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVGNGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            complement(38325..38606)
                     /locus_tag="IU325_03970"
     CDS             complement(38325..38606)
                     /locus_tag="IU325_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HAP5808196.1"
                     /translation="MRLEWKPMSLSDREQIMEYISKDNPLAAIALDNQFEAIAEQACE
                     RPEMYRAGRVAGTHEAVVRPHYVLVYQVEGDTLSVLRVLHTALQWPPAE"
     gene            complement(38606..39046)
                     /locus_tag="IU325_03975"
     CDS             complement(38606..39046)
                     /locus_tag="IU325_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808197.1"
                     /translation="MAPETIDHSTLHKLVESGVVDAAHVIGTQGGWSLTVKYGHTERP
                     LAAQRSRQIRLFKRLETVVNYLKDVGIARFEVDASNYDPDGQKKTTRPDRAEALKRAH
                     EAAAYDAWFREQVQAAIDDPRPALSHEEAKSLFAARKKALLKGD"
     gene            complement(39203..40567)
                     /gene="rhlE"
                     /locus_tag="IU325_03980"
     CDS             complement(39203..40567)
                     /gene="rhlE"
                     /locus_tag="IU325_03980"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HAP5808198.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRAESGAKSGNAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            40796..41467
                     /gene="cecR"
                     /locus_tag="IU325_03985"
     CDS             40796..41467
                     /gene="cecR"
                     /locus_tag="IU325_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HAP5808199.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            41467..42465
                     /gene="hlyD"
                     /locus_tag="IU325_03990"
     CDS             41467..42465
                     /gene="hlyD"
                     /locus_tag="IU325_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HAP5808200.1"
                     /translation="MMKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRT
                     VNLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLML
                     AGYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQA
                     TLKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTR
                     AVEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQ
                     IGFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            42458..44194
                     /locus_tag="IU325_03995"
     CDS             42458..44194
                     /locus_tag="IU325_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP5808201.1"
                     /translation="MNDAVITLNGLEKRFPGMNKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFSRLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPTALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPITRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            44187..45320
                     /locus_tag="IU325_04000"
     CDS             44187..45320
                     /locus_tag="IU325_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP5808202.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            45331..46437
                     /locus_tag="IU325_04005"
     CDS             45331..46437
                     /locus_tag="IU325_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP5808203.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(46399..46809)
                     /locus_tag="IU325_04010"
     CDS             complement(46399..46809)
                     /locus_tag="IU325_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808204.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            46942..47703
                     /locus_tag="IU325_04015"
     CDS             46942..47703
                     /locus_tag="IU325_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EEP domain-containing protein"
                     /protein_id="HAP5808205.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            47700..48941
                     /gene="clsB"
                     /locus_tag="IU325_04020"
     CDS             47700..48941
                     /gene="clsB"
                     /locus_tag="IU325_04020"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HAP5808206.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDAHIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            48941..49897
                     /locus_tag="IU325_04025"
     CDS             48941..49897
                     /locus_tag="IU325_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HAP5808207.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(49933..50322)
                     /locus_tag="IU325_04030"
     CDS             complement(49933..50322)
                     /locus_tag="IU325_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808208.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGMVATAELALVIIMD
                     AALMSERPVWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLY
                     YYFIGFFGILAAIAITLVWQRHTRFFH"
     gene            complement(50527..51231)
                     /locus_tag="IU325_04035"
     CDS             complement(50527..51231)
                     /locus_tag="IU325_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
                     /protein_id="HAP5808209.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(51368..51820)
                     /gene="moaE"
                     /locus_tag="IU325_04040"
     CDS             complement(51368..51820)
                     /gene="moaE"
                     /locus_tag="IU325_04040"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HAP5808210.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(51822..52067)
                     /gene="moaD"
                     /locus_tag="IU325_04045"
     CDS             complement(51822..52067)
                     /gene="moaD"
                     /locus_tag="IU325_04045"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HAP5808211.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSLDHPLTDGDEVAFFPPVTGG"
     gene            complement(52060..52545)
                     /gene="moaC"
                     /locus_tag="IU325_04050"
     CDS             complement(52060..52545)
                     /gene="moaC"
                     /locus_tag="IU325_04050"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HAP5808212.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(52548..53060)
                     /gene="moaB"
                     /locus_tag="IU325_04055"
     CDS             complement(52548..53060)
                     /gene="moaB"
                     /locus_tag="IU325_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HAP5808213.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(53082..54071)
                     /gene="moaA"
                     /locus_tag="IU325_04060"
     CDS             complement(53082..54071)
                     /gene="moaA"
                     /locus_tag="IU325_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HAP5808214.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDAHQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(54059..54203)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            54468..55376
                     /gene="yvcK"
                     /locus_tag="IU325_04065"
     CDS             54468..55376
                     /gene="yvcK"
                     /locus_tag="IU325_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HAP5808215.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTAP
                     IQELLLTPNVPATREAVHAISEADLIIIGPGSFYTSLMPILLLNEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(55529..57550)
                     /gene="uvrB"
                     /locus_tag="IU325_04070"
     CDS             complement(55529..57550)
                     /gene="uvrB"
                     /locus_tag="IU325_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HAP5808216.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
BASE COUNT        13629 a        15442 c        14836 g        13689 t
ORIGIN      
        1 ggcaggagcc agcgctacgc ccgaggtggc atacagtctg tggcgttttt ctgacataat
       61 aggcaaattc gattttgcct ccgcaggagt gttttcatgg aatttaccac cggattgatg
      121 tcgctcgaca ccgcgcttaa tgagatgctt tctcgcgtca ccccactgac cgcccaggaa
      181 acgctgccac tggtacagtg ttttggtcgt attctggcga gcgatgtcgt ttcgccgctt
      241 gatgtcccgg ggtttgataa ctccgctatg gacggctacg cggtgcgttt agccgatatt
      301 gcctccgggc aaccgctgcc cgttgccggt aaatcctttg ccggtcagcc gtaccatggt
      361 gaatggcctg cgggtacctg cattcgtatt atgaccggtg cgccggtgcc agaaggctgc
      421 gaagcggtgg tcatgcagga acagactgaa caaacggaca atggcgtgcg ttttactgct
      481 gaagtgcgta gcgggcaaaa tattcgccgt cgcggtgaag atatctctgc ggatgcggtt
      541 gttttcccgg cgggaacacg cctgactacc gcagagctgc cagtaattgc ttcactgggg
      601 attgccgaag ttccggtgat tcgtaaagtg cgtgtagcgc tgttttctac cggtgatgaa
      661 ctccagttgc caggccagcc gctgggcgac ggccaaatct acgataccaa ccgtctcgcc
      721 gtacacctga tgttagaaca gctgggatgc gaggtaatca acttagggat tatccgcgac
      781 gatccccatg ccctgcgcgc cgcttttatt gaagccgata gtcaggcgga tgtggtgatc
      841 agttccggcg gtgtttcagt gggtgaagcg gattacacca aaaccattct tgaagagctg
      901 ggggagatcg ccttctggaa gctggcgatt aaaccaggta aaccgttcgc gttcggaaaa
      961 ctttctaata gctggttctg cggcctgccg ggcaacccgg tttcagcaac gctgaccttc
     1021 tatcaactgg tacagccttt gctggcaaaa ctaagcggca ataccgccag cggcctgccc
     1081 gcgcgccagc gtgtgcgcac ggcatcgcct ctgaagaaat cgccaggacg ccttgatttc
     1141 cagcgcggcg tgctgcaacg caacgccgat ggcgaactgg aagtgacgac caccggacat
     1201 cagggttcac atatatttag ctcctttagc ctcggcaact gctttatcgt actggagcgc
     1261 gagcgcggca atgtagaagc gggcgaatgg gtggaagtag aaccgtttaa cgcgttgttc
     1321 ggaggcctgt aatggcggaa ctcagcgatc aggagatgtt gcgctacaac cggcaaatca
     1381 ttctgcgcgg ttttgatttt gacggtcagg aggcgctaaa agattctcgt gtgctgatcg
     1441 tcggcctcgg tggactcggc tgcgcagctt cgcagtatct ggcaagcgcc ggtgtcggca
     1501 acctgacgct gctcgacttc gacacggttt cgctctccaa tctgcaacgc cagacgctgc
     1561 acagtgatgc cacggtcggg caaccgaaag tggaatccgc ccgtgacgcc ctgacgcgga
     1621 tcaacccaca tatcgcgatt acacccgtca atgcactgct agatgacgca gaacttgcag
     1681 cattgattgc tgaacacgat ctggtgctcg actgtacgga taacgttgcg gtacgcaatc
     1741 aactgaacgc aggctgtttt gccgcgaagg tgccgctggt ttccggcgcg gcaattcgta
     1801 tggaaggtca aatcacggtc ttcacttatc aggacggcga gccttgctat cgctgcctta
     1861 gccgtttgtt tggcgagaac gcgttaacct gcgtagaagc aggcgtgatg gcaccgttga
     1921 tcggcgtaat tggttcgttg caggcaatgg aagcgatcaa actgctggca ggttatggca
     1981 aaccggccag cgggaaaatc gtcatgtacg atgcgatgac ctgcctgttt cgtgaaatga
     2041 aactgatgcg taacccgggg tgtgaggtgt gtgggcagta attgccacct caggccagat
     2101 aagacgtagc aggcatcggt gcactccgcc acgtcatcag caatttatga ggatgcagaa
     2161 cgtccggtta aatcgacgtt ctgccaaacg ctcccagcca gtcctgttca aacttcgcca
     2221 cagcggcatc aaccgccgga tagctaatca tctgttgtgc cacatccagc ggcagagtaa
     2281 ttgattcaca ccctgccagt aagcagtcca gcgcctgacg cggggttttg aaactcgcgg
     2341 ctaacacttt cgcctgcggc gcatgcattt tcaataactg gtgtaagtcg gtcacagtct
     2401 gaataccgct accgccctga gcatcaatac gattaacgta aggcgcaaca tattccgcac
     2461 ctgccagcgc cgacagtagc ccttgtgccg cgccatatac cgcggttccc agcgtcggaa
     2521 tcccttccgc ttttaacatc ttaatagctg ccagcccctc agcggtcacc ggaactttca
     2581 ccacgatatc cgcaataata gaacgcaatt tacgtgcgtc attcaccatc ccttcagcag
     2641 tggtagccat tacctgggca aacagacgcc cctgaccgcc catcgcttca tgaagttccg
     2701 gaagcacaac ctccagcggt tttttacccg cggcgataat gcttgggtta gtggtcacac
     2761 ccgccagcgg aaaaatacgt gacagcgcct tcaccgcaac aacgtctgaa gtatccagat
     2821 acagttccat aaccatcctc aaaatgtact ttttgtctga atgtagacta tcacgggaaa
     2881 actctctgaa cagttgacgc acatcaatct aactttcatt cgaaagtatt ttaatgtttg
     2941 aacgaaacag agaggcaact atgattttca atattcagcg ctactcgacc catgatggcc
     3001 ccggtatccg cacggtcgta tttcttaaag gctgttcgct gggctgccgc tggtgtcaga
     3061 acccggaaag ccgcgcccgc acgcaggatc tgctgtatga cgcacgactg tgtctggaag
     3121 gctgcgagct gtgcgctaag gccgcgccgg aagtgattga gcgcgcgctg aatggcttgc
     3181 ttattcatcg ggaaaagtta accccggagc atctgacagc gttaaccgac tgctgcccga
     3241 cacaggcgtt aaccgtatgt ggtgaagtga aaagcgttga ggagataatg aagaccgttc
     3301 tgcgcgataa accgttttac gatcgcagcg gcggcggctt aacgctttct ggcggtgaac
     3361 cgtttatgca gccggaaatg gcgacggcgc tactgcaagc cagccacgag gccggcattc
     3421 atactgcggt agaaacctgt ctgcatgtgc cgtggaaata tatcacccct tctctgccct
     3481 atatcgatct gtttcttgcc gatttaaaac acgttgccga cgcaccgttt aaacaatgga
     3541 ccgacggtaa cgccgccagg gtgctggata acctgaaaaa actcgccgca gcgggcaaaa
     3601 aaatcattat ccgcgtgccg ctgattcagg gctttaatgc cgacgaaacc tctgtaaaag
     3661 ccattaccga ttttgctgcc gacgagctgc acgttggcga aattcatttc ctgccctacc
     3721 acacgctggg catcaacaaa tatcacttac ttaatctgcc ctatgacgcc ccggaaaaac
     3781 cgcttgatgc gccagaactg ctcgactttg cccagcagta tgcctgccag aaagggttaa
     3841 ccgcgacctt acgaggataa caaccatgac cacactgaaa ctggacacgc tcagcgaccg
     3901 cattaaagcg cacaaaaatg cgctggtgca tattgtgaaa ccgccggtct gtaccgagcg
     3961 cgcgcagcac tatactgaga tgtatcaaca acatctcgat aagccgatcc cggtacgtcg
     4021 cgcgctggcg ctggcgcatc acctggcgaa tcgcactatc tggatcaagc acgatgagtt
     4081 gatcattggc aaccaggcaa gcgaagttcg cgccgcgccg atcttcccgg aatatactgt
     4141 ctcgtggatc gaaaaagaga ttgatgatct ggcagatcgt cccggtgctg gctttgcggt
     4201 gagcgaagag aacaaacgcg ttctgcatga agtgtgcccg tggtggcgcg gtcagaccgt
     4261 acaggatcgc tgctacggca tgtttaccga tgagcaaaaa ggtctgctgg cgaccggcat
     4321 cattaaagcg gaaggcaata tgacctccgg cgatgcgcac ctggcggtca atttcccgct
     4381 gctgctggaa aaagggcttg atggtctgcg cgagaaagta gcggaacgtc gctcgcgcat
     4441 caacctgacg gtgctggaag atttacacgg tgagcaattc ctgaaagcga ttgatatcgt
     4501 gctggtggca gtcagtgaac acattgaacg tttcgctgcc ctggcgcgtg aaatggccgc
     4561 gaccgaaact cgcgaaagcc gtcgcgatga gctgctggcg atagcagaaa actgcgatct
     4621 tatcgcccac caaccgccgc agactttctg gcaggcgctg caactgtgtt acttcatcca
     4681 gttgattttg cagattgaat ctaacggtca ctccgtatct tttggtcgta tggaccagta
     4741 tctgtacccg tattatcgtc gcgacgttga actcaaccag acgctggatc gtgaacacgc
     4801 catcgagatg ctgcatagct gctggctgaa attgctggaa gtgaacaaga tccgctccgg
     4861 ctcacactca aaagcctctg cgggaagtcc gctgtatcag aacgtcacta ttggcgggca
     4921 aaatctggtt gatggtcaac caatggacgc ggtgaatcca ctctcttacg cgatcctcga
     4981 atcctgcggt cgcctgcgtt ccactcagcc taacctcagc gtgcgttacc atgcaggaat
     5041 gagcaacgat ttcctcgacg cctgcgtaca ggtgatccgt tgcggcttcg ggatgccggc
     5101 gttcaacaac gacgaaatcg tgatcccgga atttattaaa ctcggtattg aaccgcagga
     5161 cgcttacgac tatgcagcga ttggttgtat cgaaaccgcc gtcggtggca aatggggcta
     5221 tcgctgtacc ggcatgagct ttatcaactt cgcccgtgtg atgctggcgg cgctggaagg
     5281 cggtcgtgat gccaccagcg gcaaagtgtt cctgccacaa gaaaaagcgt tgtcggcagg
     5341 taacttcaac aacttcgatg aagtgatgga cgcgtgggat acgcaaatcc gttactacac
     5401 ccgcaaatca atcgaaattg agtatgtcgt cgacaccatg ctggaagaga acgtacacga
     5461 tattctctgc tcggcgctgg tggatgactg tattgagcga gcgaaaagta tcaaacaagg
     5521 cggcgcgaaa tatgactggg tttctggcct gcaggttggc attgccaacc tcggcaacag
     5581 cctggcggca gtgaagaaac tggtatttga acagggcgcg attggtcagc aacagctggc
     5641 tgccgcactg gcggatgact tcgatggcct gactcatgag caactgcgtc agcggctgat
     5701 taacggtgcg ccgaagtacg gcaacgacga tgataccgtc gatacgctgc tggctcgcgc
     5761 ttatcagacc tatatcgacg aactgaaaca gtaccataac ccacgctacg gtcgtggtcc
     5821 ggttggcggc aactattacg cgggtacgtc gtctatctcc gctaacgtac cgtttggcgc
     5881 gcagactatg gcaacaccgg acgggcgtaa agcccacacc ccgctggcag aaggcgcaag
     5941 cccggcttcc ggtactgatc atcttggccc tactgcggtc attggctcgg tgggtaaact
     6001 gcctacggca gcgattctcg gcggcgtgtt gctcaaccag aaactgaatc cagcaacact
     6061 ggagaacgaa tctgacaagc agaaactgat gatcctgctg cgcaccttct tcgaggtgca
     6121 taaaggctgg catattcagt acaacatcgt ttcccgcgag acgctgctgg aagcgaaaaa
     6181 acatccggat cagtaccgcg atctggtagt gcgtgtcgcg ggttattccg ccttcttcac
     6241 cgcgctctct ccagacgctc aggacgatat catcgcccgt actgaacata tgctgtaatc
     6301 ttctcagccc ggcggcgtcg tcgccgggct aaatcattca caccatcaat tttagcctcg
     6361 ctttcattcg aaatataatt tgtgctctgc gtcacattgt tattacactg ttacggtttc
     6421 attttcgagc caggagcaca cctatgagcg taaaagttat cgtcacagac atggacggta
     6481 cttttcttaa cgacgccaaa acgtacaacc aaccacgttt tatggcgcaa tatcaggaac
     6541 tgaaaaagcg cggcattgag ttcgttgtcg ccagcggtaa tcagtattac cagcttattt
     6601 cattctttcc tgagctaaag gatgagatct cttttgtcgc ggaaaacggc gcactggttt
     6661 acgaacatgg caagcaactg ttccacggcg aactgacccg acatgaatcg cggattgtta
     6721 ttggcgagtt gctaaaagat aagcaactca attttgtcgc ctgcggtctg caaagtgcat
     6781 atgtcagcga aaatgccccc gaagcattcg tcgcactgat ggcaaaacac taccatcgcc
     6841 tgaaacctgt aaaagattat caggagattg acgacgtact gttcaagttt tcgctcaacc
     6901 tgccggatga acaaatcccg ttagtgatcg acaaactgca cgtagcgctc gacggcatta
     6961 tgaagccggt taccagtggt tttggcttta tcgacctgat tattcctggc ctgcataaag
     7021 cgaatggtat ttcgcggtta ctgaaacgct gggatctgtc accgcaaaat gtggtagcga
     7081 ttggcgacag cggtaacgat gcggagatgc tgaaaatggc gcgttattcc tttgcgatgg
     7141 gcaatgctgc ggaaaacatt aaacaaatcg cccgttacgc taccgatgat aataatcatg
     7201 aaggcgcgct gaatgtgatt caggctgtgc tggataacac atcccctttt aacagctgac
     7261 ctctctaccg gagccatgcg ctccggtttt cccttctttt tacatcctgt acatcacatc
     7321 aaatcattaa acttgcatta acttatttat aacttatatt atcacatgca aatcattata
     7381 gtttcgaatg aaagttaatg cgaggtggtt atggcttcta cttttaccag cgacacattg
     7441 cctgccgatc acaaagcagc tatccgtcag atgaagcacg cgctgcgggc gcagcttggc
     7501 gacgtccagc agatctttaa tcagctaagc gatgacattg ccacgcgagt ggctgaaatc
     7561 aacgcactca aagcacaggg cgatgccgtc tggccggtgc tgtcttatgc cgatatcaaa
     7621 gcaggtcatg ttactgcaga gcagcgcata cagattaaac gtcgcggttg tgcggtgata
     7681 aaaggccatt tcccccgcga acaagcgcta ggctgggatc agtcgatgct ggactatctg
     7741 gaccgcaacc gctttgacga ggtctacaaa ggccccggcg ataatttctt cgggacgctc
     7801 agcgcttcac gtcccgagat ttaccccatc tactggtcgc aggcgcaaat gcaggctcgc
     7861 cagagtgaag aaatggcgaa tgcgcagtcg tttctcaatc gtctgtggac atttgaaagt
     7921 gatggaaagc aatggtttaa cccggatgtg agcgtcatct accctgaccg tatccgccgc
     7981 cgtccgcccg gaacgacctc caaaggtctt ggagcgcata ccgactccgg ggcactggaa
     8041 cgctggctgc ttccagcgta tcagcgcgtt ttcgccaacg tctttaatgg caatctggcg
     8101 caatatgatc cctggcatgc ggcacatcgt acggaagttg aagagtacac ggtggacaac
     8161 accaccaaat gttccgtgtt tcggacattc cagggctgga cagcgctctc tgatatgctg
     8221 cctggtcagg ggctgctgca cgtcgtgccc attcctgaag ccatggcgta cgtactgtta
     8281 cgtccgctgc ttgatgatgt gccggaggat gaactgtgcg gcgtagcgcc cggaagagta
     8341 ttgccggtat cagagcaatg gcatccactg ttgattgagg cgttaaccag cattccaaaa
     8401 ctcgaagccg gagactccgt ctggtggcac tgcgacgtca tccattccgt tgcccccgtt
     8461 gaaaatcaac agggctgggg caacgtgatg tacattcctg cggcaccgat gtgcgagaaa
     8521 aatcttgcct acgcgcacaa ggtgaaggcc gcactggaaa aaggcgcatc gccgggcgac
     8581 ttcccgcgcg aggactatga aacaaactgg gaaggacgct ttacgcttgc cgacctcaac
     8641 attcacggta agcgagcgtt gggaatcgac agctgagtgt cgtacagccc ttcccgttcc
     8701 acggcggtgt tcccttttcc tgggcgaacc cgccgcactg actcccgcac cgccaatcgt
     8761 ccgtgcagca gcagcgagcc caccggagca tccgggcgaa tcagccgctg ttgcaacatt
     8821 tgcaccgctt cattgcctag ctcatcgcgt ggcacatgca cagccgtcag gggaacatcc
     8881 tcgattgccg caaggttaaa gccgtcgata ctcatcaccg agacatcctg tggtacgcgc
     8941 agaccacgac gatgtagcgc atttaccgtt cccgccgcca taaaatcccc gccgaccaga
     9001 aacgccgtcg gcaacgtctt gccattggcg gcatccagcc acgctccgac cttttcttcc
     9061 gcttctttag cgctaaaact gctgatattc agcaaatgac ggtcatggtg aaaggccaga
     9121 ttatgctgac gccatgattc tttaatccct gcaaaccgct gctccatagt gtagcggcgc
     9181 aggcacatca cattcatcac ctcccggtga cccatttcaa acagatagcg ggcggcgaac
     9241 gcgccaatta accgatggtc cggcgcaatc gccgggaggc gcattcgttc atcacggcag
     9301 ttgatcagca cacaaggctt agcgagatcg gcggccagat catggatgtg cggatcgtca
     9361 atccccagca ggatcgccgc ctgcgtatct gcttcattca tccgcgagag aaatagcgcg
     9421 ggatcgctgt caaactcctc cagcgcgcag taacgcagcc gcacttcatg cggcgataac
     9481 gctttgctga tgctctggat cacacggtaa taaaagatat cagatcgttg gtcaaaagcg
     9541 cgttgcggcg cgaaaataat caggttgttc agcaacatac gacctgctgc catgccatcc
     9601 atcacgccca actcacgcgc gcaggccagc acctgttcgc gagcgcgtgc actggtattc
     9661 gctttgccag ccagcacccg cgaaaccgta ctcggcgata gcccggtacg tgcggcgata
     9721 tcggcgattt tcagcttctt gtccattttg tgatctgcct ctaattcgga aatgacagaa
     9781 ttttcatgag caaaaaagcc cattctgtct ggcattaaat ccatttcagt aatgtatatc
     9841 agtcactcat ggaaaaacgt gcacaaaacc cgctttcttt cgcttttagg gtctcttact
     9901 gtgaattggt agaacaactt ccatacaaaa ataatggaaa aacgaacaat aaagacttta
     9961 tttatcaaaa agttaaaagt ggtgtgacaa ctccattctg acgttgccac acaaacccta
    10021 taccattctg tggagtgcat atgagtcagg gtatcaataa cgatatgact gccagtaaac
    10081 cgcgccatat cgtcaaaaat ctgcgttggt ggatactggt cctttttttg ctcggtgtta
    10141 ctgtcaacta catcacgcgt aactcgctgg gtattctcgc ccccgaactg aaaagcaacc
    10201 tggggattac caccgaacaa tattcctgga ttgtaggtgc tttccagctc gcctataccc
    10261 tattccaacc gttgtgtgga tggctgattg acgtcattgg cctcaaactg ggctttatga
    10321 tctgcgcggg actgtgggcg ctggcctgtc tgatgcatgc gggggcggga aactgggtac
    10381 atctggcgct gttgcgcttc tttatgggcg gtgcggaagc cgcggcaacc ccagcaaacg
    10441 ccaaaaccat cggtgaatgg tttccaaaat cagaacgccc gatcgccgca ggctgggcag
    10501 gcgtcggttt ttccatcggt gccatgctgg ccccaccgat tatctatttt gcccacgctt
    10561 cattcggctg gcagggtgca tttatgttca ctggcatcct ggcgatgctg tgggtggtgc
    10621 tgtggtgggc gttctatcac aacccggata agcatccgaa cctcagcaaa gccgagctgg
    10681 actttatccg ccaggataac gaagcgcccc cgattaaatt accctttctg accgcactga
    10741 aaaccgtggg caaaaataaa cgcttttacg gtatcgctat cccggccttt atggcagaac
    10801 cggcgtgggc ggtgatgagt ttctgggtgc cgctatacct gtccaaaacc tatggcatgg
    10861 acctgaagca gatcgcatta ttcgcctggt tgccgttcct cgccgctgat cttggcagta
    10921 ttgccagcgg ctatctgacc aaactttaca cccgcctgtt tgggctgagt cgtgtgaact
    10981 ccgttgtcgc atcatcggtc agtggcgcat tcttgatggt ttcgctggcg gtgatggcct
    11041 ttaccaataa cccgtatatc gccatcgtcc ttatttccat cggcggcttc ggtcaccaga
    11101 tcatctcctg catgctgagc gcactggtgg tggaatcctt cgacaaaggt cagatggcca
    11161 ctgttaacgg catgcgcggt tctgccgcgt ggatcgcaag tttcctgttc tccctgatta
    11221 tcggggttac cgctgacaaa atcggcttta accccctgtt tatcgccatg ggcttctttg
    11281 acctgattgg cgctttcttc ctggtcacct ttattgctga acgtcgcgca aaacgcgcct
    11341 gagagtggat gtaaacatga aaacgctgaa aaactggacc ctgcacaaac agtctgccca
    11401 ccacgttgaa ctgctggtcg atggccagca tatcctgtgc ctgtacgtgc tggaagaaaa
    11461 tctgttccgc gtgctgttaa aacgcaaagg cgagctggcg ctggatcgca cctggagcat
    11521 tgctccggcg caggatgtgc catgggaagg gcgcagccgc gaagatctca gcggctttag
    11581 cctgcccgcg tggacattga tccagcacga cgacaccgtg gttatcgcca ccgagtcact
    11641 gcgtgccacc gttcaccagc cgctgtggct ggagtggcac tatcgtaaca acgaaggcga
    11701 atggctgccg ttggtgaatg accgcccgac cagcgcttat ctgctgaatg cccacggcga
    11761 tggcgtggca cactatcaga gccgtcgtaa agatgaacgc ttttatggcc tcggagataa
    11821 agccggcgac ctgcaacgca acggtaaacg ttacgagatg cgtaacctcg acgcgatggg
    11881 ctacaacgcc gttagtactg acccgctgta caaacacatt ccattcacca tcacccgccg
    11941 cgatgacatt agcttcggcc tgttttatga caacctcagc agctgctggc tggatctggg
    12001 caacgagatc gacaactacc acaccgccta tcgccgctgg caggcggaag cgggtgatat
    12061 cgactactac ctgtttaccg gcgaacgcgt acttgatatc accaaagcct tcgtacgcct
    12121 gaccggcaaa accctgttcg gaccgaaatg gagcctgggc tacagcggtt caactatgca
    12181 ctacaccgat gcgccggacg cacaaaatca gttgatgaac tttatccgcc tgtgtgaaga
    12241 acatgcgatt ccctgcgatt cgttccagct ctcttccggt tatacctcaa ttaacggcaa
    12301 gcgctatgtg ttcaactgga attacgacaa agtgccgcag ccgaaagtga tgagccaggc
    12361 atttcacaat gctggtttgc acctggccgc caatatcaag ccgtgcctgt tgcaggatca
    12421 cccacgctat aacgaagtgg cagaaagagg actgttcatc cgtgattcag aaacagatga
    12481 accagaacgt tccagcttct gggatgacga aggttctaac ctcgacttta ctaatccgca
    12541 gaccatccag tggtggcagg aaggcgtgac cacacagctt ctggagatgg gtatcgactc
    12601 cacctggaac gacaacaacg aattcgaagt atgggacggt gaagcacgct gtcacggctt
    12661 tggtcagacg atcgcgatca aacatattcg cccggtgatg ccactgctga tgatgcgagc
    12721 ctcgatggaa gcccagcaac gtttcgctcc ggaaaaacgt ccgtacttga tctcccgctc
    12781 cggctgcgcg ggaatgcagc gctatgtgca gacctggagc ggcgataacc gcactagctg
    12841 ggacactctg cgctacaaca cccgcatggg tctggggatg agcctctccg ggctgtataa
    12901 cctcggtcac gacgtcggcg gtttctccgg tgataagccg gacccggaac tgtttgtacg
    12961 ctgggtacaa aacggcgtga tgcatccgcg ctttacgatt cactcgtgga acgatgacca
    13021 taccgtaaac gaaccgtgga tgtatccggg cgtcactccg gcgatccgca gcgctattga
    13081 gttgcgctat cgcctgctgc cctactttta taccctgatg tggcaggcat acgccgatga
    13141 tgaaccaatg ctgcgtccga ctttccttga ccacgagcac gacgttcaga cttttgaaga
    13201 gtgtgatgac ttcctgcttg gtcgcgatat tttggttgcc agcgtcgtag agcaaggcga
    13261 gcgccagcgc cgtgtatggc tgccagataa cgaaactggc tggtacgatt tctacaacgg
    13321 cgaatggttc agtggcggtc agtggatcac cctcgacgca ccgctggaga aactgccgct
    13381 gatggtgcgt gccggggctg gtctaccgct cagcaaacgc atcacctatg tgagcgcaga
    13441 acaagacgat acccgcgaac tgaaactgtt cccgctgaaa ggtgtgggca caacgtccgg
    13501 cctgctgttt gaagacgatg gcgaaagctg gggctaccaa actggtaatg cgctttgggt
    13561 ggaatgggaa atggtttgcg atggggcgac catcaacctg aaagtcaacg cgcgcgggga
    13621 ttatcgacct gcatggagtg cactgaaagt gtcattacca gtggaagaaa aacgcacgct
    13681 gctggtcaat ggtgttgaag ggagtgaatg gatgcgttaa atcattaccg gggccgggag
    13741 gcgactgcgc cttacccggt ccagccatcg aatcctttac ctcagtattt cacgctgcag
    13801 gaaggcagca aacctgtaaa ccccgggagc ttaaataagt aagtgaccgg ggtgaacagg
    13861 tgaagccaat gcatctgccg catgaaagac ggcgggaaat taaccgtcga tccctttact
    13921 acgcaggtaa tcttcgtaat taccgctaaa gtcgatcacg cgttccgggg tgatttccag
    13981 aattcgggtc gccagggagc ttacaaactc acggtcgtga gacacaaaga tcagcgtgcc
    14041 ctgatacagt tccagcgcca tgttcagcga ctcaatagat tccatatcca ggtggttggt
    14101 cggttcgtcc atgatcagaa tgttcggctt ctgcatcatt aacttaccaa acagcatccg
    14161 ccctttttct ccaccggaaa gcactttcgc tggctttttg atgtcgtcct ggctgaacag
    14221 caaacgaccg agaatgctgc gtaccgcctg ctcatcatcg ccttcctgct tccactggct
    14281 catccattcg aacacggtca gatcattttc aaactcatat tcgtggtcct gggcgtagta
    14341 accaatgcgt gcgttttcag accattttac ggtgccgctg tccggttgca gatcgcccac
    14401 cagcgttttc agcagcgttg atttaccgac gccgttggta cccagtacag ccagtttttc
    14461 acccacttcc agcagcaggt tgagattttt aaacagtgga ccgttatcaa accctttggt
    14521 cagaccttcc acttccagcg cgttacggaa cagtttctta tcctgttcaa aacggatgaa
    14581 cgggttctga cggctggagg ctttcacctc ttccagtttg attttatcaa tctggcgcgc
    14641 gcgcgaagtt gcctggcgag atttcgaggc gttggcgcta aagcggctaa cgaaagattg
    14701 cagctcagca atctgcgctt tcttcttggc gttatcagcc agcagacgct cacgcgcctg
    14761 ggtcgccgcc gtcatgtact catcgtagtt acctggataa acgcgcagct cgccgtaatc
    14821 cagatccgcc atgtgggtac aaaccatgtt aaggaagtga cggtcgtgcg agatgatgat
    14881 catggtgctg tcacgctcgt tcagcacctg ttccagccaa cgaatggtgt cgatgtcgag
    14941 gttgttggtc ggttcgtcga gcaggagaat gtctggatca gcaaacagcg cctgcgccag
    15001 cagcacacgc agcttccagc caggagcaac ttcactcatc gggccgtagt gctgttccac
    15061 tggaattccc acgccaagca acagttcacc ggcgcgagct tccgcagagt aaccgtccat
    15121 ttcgccgtat ttcacttcca gatcggccac tttatagccg tcttcttcac tcatttccgg
    15181 caaagcatag atgcggtcac gctcctgttt cacttcccac aactctttat gccccatgat
    15241 caccgtatcc aacacagtga actcttcaaa ggcaaactga tcctgacgca gcttaccaat
    15301 gcgctcgttg ggatcgaggg aaacgttacc caacgtcggc tctaagtcgc cgccgaggat
    15361 cttcataaag gtggatttac cactaccgtt cgcgccaatc aggccgtaac ggttgccgcc
    15421 gccaaatttg acggaaatgt tttcaaacaa cggcttactg ccgaactgca tggtgacgtt
    15481 actggaaact aacacaggcg tatcctgaaa agagatatga caaaccgcgc attatgccat
    15541 aagtctgaaa gaagatcctg ctcctcgctt aaggtctaca ctcaagccaa aggcaaaaaa
    15601 tgtgatttcg tacacatctg atttcactgt gagcaggaat gaacttataa tgcgcttcca
    15661 atactctaat attctcaacc caatggcctg ccaggcacaa aatctcgctt aacatgaata
    15721 tgaaattgaa aacattattc gcagcggcct tcgctgttgt cggcttttgc agtaccgcct
    15781 ctgcggtaac ttatcctctg ccaaccgacg ggagtcgcct ggttggtcag aatcaggtga
    15841 tcaccattcc tgaaggtaac actcagccgc tggagtattt tgccgccgag taccagatgg
    15901 ggctttccaa tatgatggaa gcgaacccgg gtgtggatac cttcctgccg aaaggcggta
    15961 ctgtactgaa cattccgcag cagctgatcc tgccggatac cgttcatgaa ggcatcgtca
    16021 ttaacagtgc agagatgcgt ctgtattact atccgaaagg aaccaacacc gttatcgtgc
    16081 tgccgatcgg cattggtcag ttaggcaaag acacgcctat caactggacc accaaagttg
    16141 agcgtaagaa agcaggcccg acctggacgc caaccgctaa aatgcacgca gagtaccgcg
    16201 ctgcgggcga accgcttccg gctgtcgttc cggcaggtcc ggataacccg atgggtctgt
    16261 atgcactcta catcggtcgc ctgtacgcta tccatggcac caacgccaac ttcggtatcg
    16321 gcctgcgcgt aagtcatggt tgtgtgcgcc tgcgtaacga agacatcaaa ttcctgttcg
    16381 agaaagtgcc ggtcggtact cgcgtacagt ttattgatga gccggtaaaa gcgaccaccg
    16441 agccagacgg cagccgttat attgaagtcc ataatccgct gtctaccacc gaagcccagt
    16501 ttgaaggtca ggaaattgtg ccaattaccc tgacgaagag tgtgcagaca gtgaccggtc
    16561 agcctgatgt tgaccaggtt gttctggatg aagcgatcaa aaatcgttcc gggatgccgg
    16621 ttcgtctgaa ttaatcgtca aaacgtaaaa cagaggcgga ctcatcatcc gcctttttta
    16681 tttgccagat cctaccaggc cgggagcata acgaataaaa cgtatcccac cagcgccgcc
    16741 cacaacagca tcggtattga atagagatgg aaacgccacc agatacggcg atcgttcgcc
    16801 atccgtaacg caatcaaatt tgccagcgat ccgggtaata aaccaaagcc ccccacgttt
    16861 accgcccatg ccagtaataa agacggcggt acataattga gcagcaaaat ggtactcggc
    16921 acgttactga tcacctgcga taaaccgatt gccgttaacc ataacccggg ttcagataaa
    16981 tgactcacgt tacccaacac accttgcaac gctggaagct gggtcagtaa atggacgtcg
    17041 ataaacatcg ccataaacac cagcagcagc gtccagtcca cactgagcac cacgcgacgt
    17101 gccagcagcg caaagcccgc cgccacaatc accagtcccc acagctcttg ttttaactcc
    17161 agcgccgtca gaaagacgat atacagcccc agacaactcc acaccagccg cggtttccac
    17221 tccggagttt gcacccccgt atggtattgc agtgcctttc cagggaaaca acaccagcac
    17281 aggagcagga gcgtcagcat cattgcgcca gccagtggtg ccatttgggc aataaatccg
    17341 gcaaacgaaa gaccagaacg tccccagata agaatatttt gcgggttgcc aattggcgtc
    17401 agtagcgaac cagcgttgac tgccagcgct tcaaaaataa tcagccgatt aaccgggatc
    17461 tcacacagtc tttttagcgt gatagtcagc ggaacaacaa taaacagcgc gacatcgttg
    17521 gtcagaaagg tagaaagcag cgccgccgcc agcaccataa acatcgccag ccgacgctcc
    17581 gtagcaaagc ggcgcaccat tttgcgcccc agcacatcaa aataaccgct taactccaca
    17641 cctttggtca gcagcatcag gccgcttaag gtgatgatgg tgtgccagtc gatagcagca
    17701 ggccaggatt tcggtgcaaa aggcacgaaa aagcttaatc cgataccaac aagaattaat
    17761 aactgaaaaa aacgatcgcc ttgcagcgtg cgtaaaaaag gcaggctcat ttggcaccgt
    17821 gtttctgggt aaacaaacga aaagcgtcga gcgtctcttc actaacatgg tgctccatgc
    17881 cttccgcgtc gcgacgggcg atttccggac tgacgcccaa caccaacaag aaattttcga
    17941 ctatctgatg tcgctcgcgg ctttcctgcg ccagcttctc tccttctgcc gttaaaaaca
    18001 cgccacgcca ggggatcatt tcaatcagcc ccatggttgc cagtcgctta agcattttag
    18061 ccaccgtcgg ttgcgaaact cccaaacgag cagccatgtc tacctgacga gcttccccca
    18121 cttccctgat caagtcagaa atcagctcaa cgtaatcatc aataagctcg cgccgatgcg
    18181 cctctctgac ctggcggaac ccttcaacgt gctcttccac gttcactaac tgcgtcactt
    18241 tttttgctgt tggcgtacct gcgcgacgac tcattgtgct tcctcattat ggtgacgcat
    18301 acatagcgtt ctcatttcgt gcgcacattg taaaccagag ttgcgaaggt acaaaaaatt
    18361 aacgttttag caatagctat ataatatagc ctgtgctata tctgtatgta atgcaatcat
    18421 ccctcaagga tcgacgggat tagcaagtca ggaggtctta tgaatgagtt caagaggtgt
    18481 atgcgcgtgt ttagtcattc tccctttaaa gtacggttaa tgctgctctc tatgttgtgc
    18541 gatatggtca acaacaaacc gcagcaagat aaaccttccg ataaatagcg gcgtcacggt
    18601 acgccgcttc actcctgctt ttatgcaggc acaacgcgtt ttggtctgaa aaaccccact
    18661 ttttgtcgga tttgcaatcc ccttcgcaaa agatttgttc gtcagtagtt gacctgaacg
    18721 gcggcgcgct ctatcttctt gcagccctgc gtatattgcg gctcgcggat gcggacccct
    18781 ttcccctctc cacgcactct tgcaggtatt gacccttgac gccagggtaa gcacatggcg
    18841 tttgttacga tagtggcata tgaatttaac cctcaaagaa tcgcttgtta cccgtagccg
    18901 ggtatttagc ccgtggactg cgttctactt tttacagtcg ctattaatta acctcggctt
    18961 aggttatccc ttcagtttgc tctacaccgc tgcgtttacg gctatgttgc ttttgctatg
    19021 gcgaacattg cctcgcgtac aaaaagttct ggtcggtgtc agttcgctgg tggcggcttg
    19081 ttatttccct tttgctcagg cctacggcgc gcctaatttc aatacattgc tggcattgca
    19141 ctccaccaat atggaagagt cgaccgaaat cctgacgatt tttccgtggt acagctacct
    19201 ggtcggctta tttatttttg cgctcggctt aatagcaatc aggcgaaaaa aagagaatga
    19261 aaaagcgcgc tggaatacct tcgacagcct gtgtctggta ttcagtgtgg cgacattttt
    19321 tgttgctccc atgcaaaacc tggcctgggg cggcgtattt aaactgaaag ataccggcta
    19381 tccggtattt cgttttgcta aggatgtcat cgtcaataat aacgaggtga ttgaagagca
    19441 agaacggatg gcaaaacttt ccggaatgaa agatacctgg acggtcactg ccgttaagcc
    19501 gaaatatcag acctatgtgg tggtgatcgg tgaaagcgcg cgtcgcgatg ccctcggtgc
    19561 ctttggcggt cactgggaca ataccccgtt tgccagcagt gttaacggtt tgatatttgc
    19621 tgactacatt gccgccagtg gctccacgca gaaatcgctt ggcttaacgc tcaatcgcgt
    19681 agtcgatggc aaaccacagt ttcaggataa ctttgtcacc ctggcaaatc gcgcgggctt
    19741 ccagacctgg tggttttcca accagggcca aatcggcgaa tacgataccg ccatcgccag
    19801 catcgccaaa cgtgcagatg aagtgtactt cctgaaagaa ggtaattttg aagcagataa
    19861 aaacacgaaa gacgaagcgt tactggatat gaccgctcaa gtgctggcac aagagcactc
    19921 gcaaccgcag ctgattgttt tacatctgat gggctcgcat ccgcaggcct gcgacaggac
    19981 acaaggcaaa tacgaaacct ttgtgcaatc gaaagaaacg tcgtgctatc tctataccat
    20041 gacgcaaaca gacgatttac tgcgtaagct gtacgatcag ttacgcaaca gcggcagcag
    20101 cttctcgctg gtttactttt ctgaccacgg tctggccttt aaagagcgcg gcaaagacgt
    20161 gcaatacctt gcccatgatg ataaatatca gcaaaatttc caggtgcctt ttatggtcat
    20221 ttcgagcgac gataaagcgc atcgggtgat taaagcccgc cgctctgcca atgacttctt
    20281 aggctttttc tcccagtgga cgggaattaa agcgaaggaa attaacatca aatacccgtt
    20341 tatatctgag aagaaagccg ggccgatata catcaccaac ttccagttac agaaggtgga
    20401 ttacaaccat ctcggaaccg atattttcga cccgaaacct taaaaacaaa aatccgcccc
    20461 gagaggcgga ttttttatat caccgaagtg attagaagcg gtaaccaaca ccggcaatcc
    20521 aggtgcctac gtcaacgcta cgaatacggc tctgctcgta agagaagtcc agagcaacgt
    20581 tttccatcgg gttgaactgc agaccagcac cgtaggagaa accgtagtcg ctggtgtcgt
    20641 gtttgtaggt cgggtattca gtggtctgga atttaccata acccacaccc actacaccgt
    20701 agatgcttgc ccagtcgtta atgcggtaag ccggaccagc agtgatgccg tagtactggt
    20761 ttttgttgta gtcaccagag cttgcagtac ggcttttctc ggtgtaagtg aaagaaccga
    20821 tcacacccag cgggctgttg tcttcttcat agcggtattt caggttgaaa ccgcccattt
    20881 tgttcatttg gccctgagcg tcgctctgtg cgtaaccgcc agttacggta gaagttgcag
    20941 ctacggaagt acctgcggtg aaagccagaa ctgcggccag tgctgaaaga catgcaattt
    21001 ttttcataac cacctcaaat gtgattcaaa taagtcctaa gttttaaata tatcaaaaat
    21061 taatgggaaa ctcttcgcga tttgtgatgt ctaacgggcc atttcatgta acagaacgtt
    21121 tccatacacc gctatccatc taaatttaaa tcactttttc agagaactgc gtaagtatta
    21181 cgcatgtttt ccctgtcatt catccagatt attcctaatc accagactaa tgattccatc
    21241 aatcctggcg cattttagtc aaaacggggg aaaatttttt caacaaatgc tcaaccagca
    21301 ttgggtatat ccagtacact ccacgcttta cttaagtcta gatatttgtg ggagaaagga
    21361 tgcctggttc attacgtaaa atgccggtct ggttaccaat agtcatattg ctcgttgcca
    21421 tggcgtctat tcagggtgga gcctcgttag ctaagtcact ttttcctctg gtgggcgcac
    21481 cgggtgtcac tgcgctgcgt ctggcattag gaacgctgat cctcatcgcg ttctttaagc
    21541 catggcgact gcgctttgcc aaagagcaac ggttaccgct gttgttttac ggcgtttcgc
    21601 tgggggggat gaattatctt ttttatcttt ctattcagac ggtaccgctg ggtattgcgg
    21661 tggcgctgga gttcaccgga ccactggcgg tggcgctgtt ctcttctcgt cgcccggtag
    21721 atttcgtctg ggttgtgctg gcggttcttg gtctgtggtt cctgctaccg ctggggcaag
    21781 acgtttccca tgtcgattta atcggctgtg cgctggcact gggggccggg gcttgttggg
    21841 ctatttacat tttaagtggg caacgcgcag gagcggaaca tggccctgcg acggtggcta
    21901 ttggttcgtt gattgcagcg ttaattttcg tgccaattgg agcgcttcag gctggtgaag
    21961 cactctggca ctggtcggtt attccattgg gtctggcggt cgctattctc tcgaccgctc
    22021 tgccttattc gctggaaatg attgccctca cccgtttgcc aacacggaca tttggtacgc
    22081 tgatgagcat ggaaccggcg ctggctgccg tttccgggat gattttcctc ggagaaacac
    22141 tgacacccat acagctgctg gcgctcggcg ctatcatcgc cgcttcaatg gggtctacgc
    22201 tgacagtacg caaagagagc aaaataaaag aattagacat taattaaatt tacatttctg
    22261 catggttatg cataaccatg cagaatttct cgctactttt cctctacacc gtctttatat
    22321 atcgaattat gcaaaagcat atttattccg aaaattcctg gcgagccaat aaataagaat
    22381 tgttcttatc aatataacta acccattgaa tttttattag ttttgttttt cacgcttgtt
    22441 accactatta gtgtgatagg aacagccaga atagcggaac acatagccgg tgctatactt
    22501 agtctcgtta attactggga cataacatca agaggatatg aaattatgag taccgctaaa
    22561 ttagttaaat caaaagcgac caatctgctt tatacccgca acgatgtctc cgacagcgag
    22621 aaaaaagcaa cagtagagtt gctgaatcgc caggttatcc agtttattga tctttctttg
    22681 attaccaaac aagcgcactg gaacatgcgc ggcgctaact tcattgccgt acatgaaatg
    22741 ctggatggct tccgcaccgc actgatcgat catctggata ccatggcaga acgtgcagtg
    22801 cagctgggcg gtgtagctct ggggaccact caagttatca acagcaaaac tccgctgaaa
    22861 agttacccgc tggacatcca caacgttcag gatcacctga aagagctggc tgaccgttac
    22921 gcaatcgtcg ctaatgacgt acgcaaagcg attggcgaag cgaaagatga cgacaccgca
    22981 gatatcctga ccgccgcgtc tcgcgacctg gataaattcc tgtggtttat cgagtctaac
    23041 atcgaataaa tccatcgttg atggtgcaga actttagtac acgataaaag cggcttcctg
    23101 acaggaggcc gttttgtttt gtagcccgcc tcaacgcact tatttagtgc atccatctgc
    23161 tatccccagc tggttaagta aattttttgt atccacatca tcacacaatc gttacataaa
    23221 gattgttttt tcatcaggtt ttacgctaaa taatcactgt gttgagtgca caattttagc
    23281 gcaccagttt ggtgccccag aatggtgcat cttcagggta ttgccctata aatcgtgcat
    23341 cacgtttttg ccgcatctcg aaaaatcaat gagttgcaaa actggcacga ttttttcata
    23401 tatgtgaatg tcacgcaggg gatcgtcccg tggatagaaa aaaggaaatg ctatgaagtc
    23461 tgtattaaaa gtttcactgg ctgcactgac cctggctttt gcggtttctt ctcatgccgc
    23521 ggataaaaaa ttagttgtcg cgacggatac cgccttcgtt ccgtttgaat ttaaacaggg
    23581 cgataaatac gtgggctttg acgttgatct gtgggctgcc atcgctaaag aactgaagct
    23641 ggattacgaa ctgaagccga tggatttcag tgggatcatt ccggcactgc aaaccaaaaa
    23701 cgtcgatctg gcgctggcgg gcatcaccat taccgacgag cgtaaaaaag cgatcgattt
    23761 ctctgacggc tactacaaaa gcggcctgtt agtgatggtg aaagctaaca ataacgatgt
    23821 gaaaagcgtg aaagatctcg acgggaaagt ggttgctgtg aagagcggca ctggctccgt
    23881 tgattacgcg aaagcaaaca tcaaaactaa agatctgcgt cagttcccga acattgataa
    23941 cgcctatatg gaactgggca ccaaccgcgc agatgccgtt ctgcacgata cgccaaatat
    24001 tttgtacttc atcaaaaccg ccggtaacgg tcagttcaaa gcggtaggtg actctctgga
    24061 agcacagcaa tacggtattg cgttcccgaa aggtagcgac gagctgcgtg acaaagtcaa
    24121 cggcgcgttg aaaaccctgc gcgagaacgg aacttacaac gaaatctaca aaaaatggtt
    24181 cggtactgaa ccgaaataat aacgctacgc ctgtaaagcg cactggcagt tccctctccc
    24241 ctctggggag aggattaggg tgaggggcgc aaacccgctc cggggccatt aattaccctg
    24301 aatttgatta tttacaccac ggtaacagga acaacatatg cagtttgact ggagtgccat
    24361 ctggcctgcc attccgcttc tgattgaagg tgccaaaatg accctgtgga tttcggtcct
    24421 cggtttggct ggcggtctgg tgatcggatt gctggcaggt tttgcacgca ccttcggtgg
    24481 ttggatagcc aaccacgtcg cgctggtctt tattgaagtg atccgcggca cacctatcgt
    24541 cgtccaggtg atgtttattt atttcgccct gccgatggcg tttaacgact tacgcatcga
    24601 cccgtttact gcggcggtgg tcaccatcat gatcaactcc ggcgcgtaca ttgcggaaat
    24661 cacgcgtggt gcggtgctgt ctattcacaa aggttttcgt gaagcaggac tggcgctcgg
    24721 tctttcacgt tgggaaacca ttcgctacgt cattttaccg ctggcactgc gtcgtatgct
    24781 gccgccgctg ggtaaccagt ggatcatcag tataaaagat acctcgctgt ttattgttat
    24841 cggcgtggcg gaactgaccc gtcaggggca agaaattatt gccggtaact tccgcgccct
    24901 tgagatctgg agcgccgtgg cggtgttcta tctgattatt accctggtgc tgagctttat
    24961 tctgcgtcgt ctggaaagaa ggatgaaaat cctgtgattg aatttaaaaa cgtctccaag
    25021 cattttggtc caacccaggt gctgcacaat atcgatttga acattgctca gggcgaagtc
    25081 gtggtgatta tcgggccgtc cggttccggt aaatcgaccc tgctgcgctg catcaacaaa
    25141 ctggaagaaa tcacttccgg cgatctgatt gtcgatggcc tgaaggttaa cgatccgaaa
    25201 gttgacgagc gcctgattcg ccaggaagca ggtatggtgt tccagcagtt ttacctcttc
    25261 ccgcatctga cggcgctgga aaacgtcatg tttggtccgc tacgcgtgcg tggcgcgaac
    25321 aaagaagagg cggaaaaact ggcgcgtgag ctgctggcga aagtcggcct ggcagaacgc
    25381 gctcaccact acccttccga gctttctggt ggtcagcaac agcgtgtggc gattgcccgt
    25441 gcgctggcgg taaaaccgaa aatgatgctg ttcgatgaac cgacttccgc tcttgacccg
    25501 gaactgcgcc atgaagtgct gaaggttatg caggacctgg ctgaagaagg gatgacgatg
    25561 gtgatcgtga cccacgaaat cggttttgcc gagaaagtag cttcgcgcct gatctttatc
    25621 gacaaaggcc ggattgcgga agatggcgat ccgcaggtgt taatcaagaa cccaccgagc
    25681 cagcgcttgc aggaattttt gcagcacgtc tcttaacttc tcagcctccc tttcccccga
    25741 aagggaggca caccagattc ctctcattta aaatcgcccc tcccccagca tctatactta
    25801 tctttttgct ctgttttctc actggaggag tcatgcggtg gatcctgttc atcctcttct
    25861 gcctgctggg cgcacctgcc cacgcggtat ccatacccgg cgttacaacc acaacgacaa
    25921 cggactcaac gactgaaccg gccccggaac cggatatcga acaaaaaaaa gcggcctatg
    25981 gcgcactggc ggatgtgctg gataatgaca cctcgcgtaa agagttgatc gaccagttgc
    26041 gcaccgtcgc cgcgacgccc cctgcggaac ccgtaccgaa gatcgtgccg ccgacgctgg
    26101 ttgaagagca aaccgtgctg caaaaagtca ccgaagtcag ccgccattat ggtgaagccc
    26161 tttccgcccg cttcggacaa ctttatcgca atatcaccgg ctccccgcat aagccgttta
    26221 atccacaaac cttcagcaat gcgttgaccc atttttcaat gttagcggta ttagtgtttg
    26281 gtttttactg gctgattcgt ctgtgcgcac tgccgctgta tcgcaaaatg ggccagtggg
    26341 cgcggcaaaa aaatcgcgaa cgcagcaact ggttgcagct tccggcgatg attatcgggg
    26401 cgtttattat cgacctgctg ttactggcac tgacattatt tgtcggtcag gttttaagcg
    26461 acaacctgaa tgcgggcagt cgcaccatcg ctttccaaca aagtttgttt ctcagcgcct
    26521 ttgccctgat tgaatttttc aaagccgtac tacgcctgat tttttgccca aacgtggcgg
    26581 aactgcgccc gttcacgata caggacgaga gcgcccgtta ctggagtcgc cgcctgagct
    26641 ggttaagcag tttgataggt tatggcctga ttgtggccgt gccgattatc tctaatcagg
    26701 tgaatgtaca aattggcgcg ctggcgaacg tcatcattat gctgtgcatg accatctggg
    26761 cgttgtacct gatctttcgt aataaaaaag agattaccca acatttgctc aactttgcgg
    26821 agcattcgct ggcctttttc agcttgttta tccgcgcctt tgcgctggtg tggcactggc
    26881 tggcaagcgc ctattttatc gtgctgtttt tcttttcgtt gttcgatccg ggcaacagcc
    26941 tgaaatttat gatgggtgca acggtgcgca gcctggcgat tattggtatc gcagcatttg
    27001 tttccggtat gttttcccgc tggctggcaa aaaccatcac cctctcgcca catactcagc
    27061 gtaactatcc agaactgcaa aaacggttga atggctggct gtcggcggcg ctgaaaacgg
    27121 cgcgtattct gacagtctgc gtggcggtaa tgttgctgtt gagcgcatgg ggattgttcg
    27181 atttctggaa ctggctgcaa aacgacgcgg ggcagaaaac tgtagatatc ctgatccgta
    27241 tcgcactcat tcttttcttc tcggcggttg gctggacagt gctcgccagt ttgatcgaaa
    27301 accggctggc ttcggatatt catggccgcc cgctacccag cgcccgtacg cgtaccctgc
    27361 tgacgctgtt tcgtaacgcg ctggcggtga ttatcagtac catcaccatc atgattgtgt
    27421 tgtcggaaat cggcgtcaat atcgcgccat tgctggcagg tgccggtgca ttggggctgg
    27481 ctatctcttt tggttcgcaa acgctggtga aagatattat caccggggta tttattcagt
    27541 ttgaaaacgg catgaacact ggagatttgg tgactatcgg gccgttgacc ggcactgtgg
    27601 aacggatgtc gattcgctcc gtgggcgtgc gtcaggatac cggggcgtat cacatcattc
    27661 cgtggtcttc gattaccacc tttgctaact ttgtccgcgg cattggttcg gtagtggcaa
    27721 attacgatgt tgatcgccat gaagatgctg ataaagccaa tcaggcactg aaagatgcgg
    27781 taacggaatt gatggaaaac gaagatattc gcgggctgat tattggtgaa ccgaactttg
    27841 ccgggattgt cggcttaagc aataccgcgt ttacactgcg tgtttcgttc accacgctgc
    27901 cactcaaaca gtggacggta cgctttgccc tcgacagcca ggtgaaaaaa catttcgacc
    27961 tggcgggcgt tcgcgcgcca gtgcagactt atcaggtgct gcctgctccg ggcgcgaccc
    28021 cggctgcacc gctgccgccg ggggagccaa cgctttaacg ttggcgatta acaaaacggc
    28081 ggcgctgctc gtcgttcata aaggtccagg caataaagcg actctgcttt tgcccctggg
    28141 ccatctcttt tttaaccacc ttcaccgcgc ccacgtccgt cagagcacga tacaacggcg
    28201 gtaagttttc accacgagat accagcgagg taaaccacat cacctgcttc gcgaagcctt
    28261 tgctctcttc aatcatcttt ttgataaagg cgacttcacc gccttcacac cacaactcct
    28321 gttgctgacc accaaagttc agtgcatcgt ctttgttcag ccccaggtta cggcgtttac
    28381 gctcactacc tgcccgcgcc gcagcggcgg aatcgtggaa tggcgggtta cacaaggtcg
    28441 cgtcgtattg ctcgttttta tggataatgc cgttaaaaat cgccccactc tctttttgcc
    28501 gacgcagacg aatggcgcgg ttaagccccg gattagcact gatgatcgcc tgcacactgc
    28561 ttaacgcctg gctgctggtt tcactaccgg taaaacgcca gccatattca tgtacgccaa
    28621 tcagcggata aatacagttc gcaccaacgc cgatatccag aatgctggca ttcgctggaa
    28681 ttgttccgct tgcttcggcc agtaaatcgg caaggtgatg aatataatcc gcccggcccg
    28741 gtactggtgg gcagagaaaa ccgtccggga tatcccagtt cgctacggcg taaaaatggg
    28801 ccagcaacgc cttattgagc gccttcaccg ccagcggatt ggcaaagtct acgctttgct
    28861 ccccggcggg tgtaagcgtg aggaattgcc tgagttcagg attgacctga caaagcgtgg
    28921 cgagatcgta gcggctgtga tgacggttgc gcggatgcaa ccccggtttc tgggcggaca
    28981 tagcattctc ctttaagcag cggcgtaaga tacccgcata acgcgacagg gtaaatatct
    29041 ggctgcgttt atcatttcta aaaaccttca catggctatg gtttatggca attcaccata
    29101 aaaatcatca tcatgcatgt tattgatttg tcttattttg taaaaatata tcgttcaaaa
    29161 aataagcgca tctctgtcaa ttttttgcgt cattttcatc gcgcaggcga cctacactta
    29221 tctggcacta cccgttgtat ttatgaggat gtcattatga aaaagtgcct cacactactg
    29281 attgccaccg tcctgagcgg aatctctctc acggcttatg ccgcgcaacc gatgagtaac
    29341 ctggacagcg gtcagctacg gcccgccggt accgtttcgg caactggcgc atcaaaccta
    29401 agcgatctgg aggataaact ggcagaaaaa gcgcgcgagc aaggcgcgaa aggttatgtc
    29461 attaattccg caggcggaaa tgaccagatg ttcggtactg caaccatcta caaataaccc
    29521 caccaacgta actgcattcg cctctggatg cagttttcgc ctgttttgta atcaaaaaac
    29581 atcaaatatg aacgcaatgt aaataaatgt atttcttttt cgcgcaatgg gtgatagaaa
    29641 atcgcgccaa gtgataatgc ttatcaaaat tattatcact tgcacgaaca ctatcacgag
    29701 attaacagtg gcatcgcatc cgcagagagg ctttctcgtg gcagtgaaaa tttcaacata
    29761 taagaaaaag tcacctgcaa aatggaaaac aatcgcaatt tccctgccag acaatttcat
    29821 tcgctcacgt tctttgccgg tctttgtatt ggtattacgc ctgtggcaca ggcactcgcc
    29881 gccgaagggc aagctaacgc ggatgacacg ctggttgtcg aagcatcaac gccttcgctt
    29941 tatgcgccac aacaatctgc agatccgaaa ttctcgcgtc cggtagcgga tactacccgc
    30001 acgatgacgg tgatttctga acaagtgatt aaagatcagg gcgcaaccaa ccttaccgac
    30061 gcactcaaaa acgtccccgg cgtgggtgcg ttttttgcgg gtgagaacgg taactccacc
    30121 actggcgacg ccatttatat gcgcggtgct gatacctcta acagtattta tattgatggc
    30181 attcgcgata tcggcagcgt ctcgcgcgac accttcaata ccgagcaggt cgaagtgatt
    30241 aaagggccgt ccggcaccga ctacgggcgc agcgcaccga caggctcgat caatatgatc
    30301 agcaagcagc cgcgcaatga ttccggcatt gacgcctccg ccagtattgg tagcgcctgg
    30361 ttccgccgcg gcacgctgga cgtcaatcag gtcattggtg ataccaccgc ggcgcgcctg
    30421 aatgtgatgg gcgaaaaaac gcacgatgcc ggacgcgaca aagtcaaaaa tgagcgttac
    30481 ggcatcgccc cttctgtcgc ttttggcctt ggtacagcga atcgtttgta tcttaattat
    30541 ctgcatgtca cccagcacaa cacgccagac ggcggcattc cgaccatcgg tttgccgggc
    30601 tattctgccc catctgcggg aacggcggcc ctgaatcatt ccggaaaagt tgatacgcat
    30661 aacttttacg gcacggattc cgattacgac gattcgacca ccgacaccgc taccatgcgt
    30721 tttgagcacg acatcaacga taacaccacc attcgcaata ctacccgttg gtcgcgcgtg
    30781 aagcaggatt acctgatgac ggcgattatg ggtggggcgt cgaatattac ccagcccacc
    30841 agcgatgtga atagctggac ctggtcacgc acggcgaata ctaaagatgt gagtaataaa
    30901 attctcacca accagaccaa cctgacctcg acgttctata ccgcttctat cggtcatgat
    30961 gtcagtaccg gcgtggaatt tacccgtgaa acgcagacta actacggcgt taatccggtg
    31021 acgttacctg cggtaaatat ttatcatcct gacagcagca ttcatcccgg cggcctgacg
    31081 cgcaacggcg caaacgccaa tggtcagacg gataccttcg caatttacgc cttcgatacg
    31141 ctgcaaatca cccgtgattt tgagctgaac ggcgggatcc gtctggataa ttatcatact
    31201 gaatatgaca gtgccaccgc ctgcggcggc agcggacgcg gtgccatcac ctgcccagct
    31261 ggtgtggcaa aaggttctcc ggtcaccacc gtcgacaccg ccaagtcggg caatctggtg
    31321 aactggaaag ccggggcgct gtatcacctg acggaaaacg gtaatgtcta tattaactat
    31381 gccgtttccc agcagcctcc gggcggcaac aacttcgccc ttgcgcagtc tggcagcggt
    31441 aacagtgcca accgcaccga ttttaaaccg caaaaagcca acaccagcga gattggcacc
    31501 aaatggcagg ttctggataa acgcctgttg ctcaccgccg cgctgttccg tactgatatc
    31561 gaaaatgaag ttgagcaaaa tgatgacgga acttactcgc aatacggtaa gaaacgcgtc
    31621 gaaggctatg agatatccgt ggccgggaat atcactcccg cgtggcaggt gattggcggc
    31681 tatacccagc aaaaagcaac catcaaaaac ggcaaagatg ttgcccagga tggttcctca
    31741 tcgctgccgt ataccccgga gcacgccttt accttatgga gccagtatca ggcaaccgat
    31801 gatatttccg ttggtgcggg cgcacgctat atcggcagta tgcataaagg ttctgacggc
    31861 gcggtgggaa cgccagcgtt taccgaaggt tattgggtcg ccgacgctaa actggggtat
    31921 cgggttaatc gcaatctcga cttccagcta aacgtctaca acctgtttga taccgattac
    31981 gtcgcctcaa tcaacaagag cggctaccgt tatcacccgg gcgagccaag aaccttcttg
    32041 ctcacggcca atatgcattt ctgattcaga tgtggggcgc aggccccact ttttggagaa
    32101 cttgtatgat gtaccacatt cccggcgtgc tatcgccaaa ggacgtcgcc cgttttcgcg
    32161 aacaactgga acaagccgaa tgggtggatg gacgcgtcac caccggcgca caaggtgcgc
    32221 aagttaagaa caatcaacag gtagacaccc gcagcacgtt atacgccgcc ctgcaaaatg
    32281 aggtgctgaa cgcggttaac caacatgctt tgttctttgc cgcggccttg ccgcgtaccc
    32341 tttccacgcc gctgtttaat cgctatcaga acaatgaaac ttatggtttt catgtggatg
    32401 gcgcagtacg cagtcaccca caaaacggct ggatgcgcac tgacctttct gccacgctgt
    32461 ttttaagcga tccacaaagc tacgatggcg gcgaactggt cgttaatgac accttcgggc
    32521 aacatcgggt aaaacttccg gcaggcgatc tggtgttgta tccctccagc agcttgcatt
    32581 gcgtgacacc cgtaacccgc ggcgtacgag tggcatcatt tatgtggatc cagtcgatga
    32641 tccgtgatga taaaaagcgc gccatgctgt ttgaactgga caacaatatt cagtcgctga
    32701 aaagccgcta cggcgaaagt gaagagatcc tgtcgctgct caatctttat cataatctgc
    32761 tgcgggaatg gtcggagatc tgacgcttga actgcccgag ttcgtcccta tattagctac
    32821 acttaactgt acaagtattg atatggggag gacgatatgg catccggttg ggctaacgat
    32881 gacgccgtca acgaacagat caacagtaca attgaagatg cgattgcccg cgctcggggt
    32941 gaaattccgc gtggcgaaag cctgtatgaa tgtgaagagt gcggagcccc catcccgcaa
    33001 gcccgtcggg aagccattcc tggtgtgcgc ttatgcattc attgtcagca ggagaaagat
    33061 ttacaaaaac cagcttatac aggatataat cgcagaggtt cgaaagacag ccagttacgt
    33121 taactacaac tggcgggatg tatagcgagt ataatttgta ttttgcgtgg cattccgtgc
    33181 ctttaacgct gtcgtgactg ccacgctgca taaaacaagt ccataagttc aacaaataaa
    33241 tggattatca gtatgttaga tatcaatcaa tttatttgaa caaggcggtc aattctcttc
    33301 gattttatct ctcgaaaaaa aacgtgatac tcatcacatc gacgaaacaa cgtcacttat
    33361 acaaaaatca cctgcgagag attaattatg aaaactatca atactgttgt tgctgctatg
    33421 gctctttcaa ctctgtcatt tggcgtgttt gccgcggaac cggtaacggc atcccaggca
    33481 cagaacatga ataaaatcgg cgtggtttct gccgatggcg catccaccct cgatgccctg
    33541 gaagcgaaac tggctgagaa agctgcggca gctggggcga gtggatacag catcacttct
    33601 gccaccaaca acaataaatt aagcggtact gcggtaattt ataagtaatt cgtaagccgg
    33661 atcaggcgtt tacgctacgt ccagcacaag tatttccctg tctgtttgcc gacagacgca
    33721 tatgctctaa ccctcattga tcctatgtta cccttgtttg cccgtccgcc actggacggg
    33781 ctttttttta gctggctaac tgctgacaga aagcctgcaa cgtctcttcc ggcataccaa
    33841 cctttcgcgc cgcatcacaa atggcctgcc agcttcccgc atccaatgga atcccctgct
    33901 cctgccgttc gcgacgcgtg ttaacttccc actcgcccgg tagcaaaatc ggcttatcgt
    33961 catcatgcgg cgaggctttc acccactcgg caaaggcttc ggtctgcgcg ctacaatccg
    34021 gcgcgccgaa cagttccggg ttgatgatga tagtggtcat gcagttaaga atggcatcgg
    34081 gactggtttg taacgttttc tgatgcgtcg ttttaccgcc ggaaagcgcc ccaccaagaa
    34141 tttcacacat cgctgcaagg gcgtagcctt tatgttcggc aaaggtcaac agcgaaccca
    34201 acggcgactc ctgcattacc gccggattgg tcgtcggcat gccgttaacg tcaatcaggc
    34261 aacctggcgg cacggggacg cctttatgcc aggcgacgcg ggttttgcca aatgcgatgg
    34321 cgctggtggc gtaatcaagc aacagcggga aattatcttt acgagggaaa accacacaga
    34381 acggattggt gccaaagcgg ctgtcgcgac cgtggaacgg cgcgaccatt ggaataccga
    34441 ccacgctaac aaagtggata gagacaaacc ccgccgctgc acactgctcc gcccagtaac
    34501 cgatacggcc gatatgatgc gagttatgga gcgccacggc ggcaatgccg tgctgatgcg
    34561 ctttctcaat ccccagcgcc atcgcttcat gtgccgcgac ctgaccaaat gcgcgatcgc
    34621 catcgagcgt gaccgccgcc cccgcctctt taacgatttt ggcatgatgg ttaatttgca
    34681 ggtgcccctg actccaggag cgcacatagc ttgggatcat gccaatacca tgtgaatcat
    34741 gccctgccag gtttgccgcg attaaatgat cggcaactaa tttcgcttct tgttcctcac
    34801 tacccatctg acgaaataca gcctgaataa aactgtgcag cgtctgagca tcaaagcgat
    34861 gaccactttc catggttaac tcctgtttat gttatgtgtt tgttgttttt ttatgctgca
    34921 cgccgggcgc gggaaggtca ataaagatgc aggaatttca tattgtggtg gggtaaaagc
    34981 cggatgacat ggctcatccg gctgagatga aaattaaaat gcctgattaa cacgcgccag
    35041 gccgtcgctg atagttgccg cttcccccgt agccaccagg cagcaagcca tctggatttt
    35101 cagcgattcg ggaatcggtt cgctgccagc caggcaacgc tcaatccact gcgccgtggt
    35161 ttccggatct tttgcttgtg gcagtaactc gctaccagca gggtcctgtt tttcatacag
    35221 cacccgcatc ccttcacggt caatgagatt gatctgcggg cagcgctgcg gattagcata
    35281 cacttcgcct tcagtaccat gcattagtaa cgcccgcccg ccgatatcgc taaagaactt
    35341 cgcgactcgc ccgatgtatt ccggatgcga aacgctggaa agacgcagtg cctcaccttc
    35401 ggcaaatggc gtcgccagtt tcgccagggt atgtgcactg ttacgcacgc ccatccgcca
    35461 gcgcatcgcc agttgttttt ccagcggcgg gcaaaacgcg ccgacaggca taaacaccgg
    35521 ttgatgttcg tcgagcttcg cctgcgcctg cccgccgtgc aacgttggcg taatacccat
    35581 cagttcgaaa atggtttctg tcagcacgcg ggttggatct tcgctaaccc cgtgaaccac
    35641 cacaggaaaa ccgagtttgt gcaggagaat cgccaacaac ggcgtcaggt tggcctgttt
    35701 acgcgcgccg ttgtaactgg ggatgacaat cggcatcggc ttgtctgcag gcggcgtcag
    35761 cttgatggtg tgattttgca tggcttcgta aaagccgagc atctctgctt ccccttcccc
    35821 tttgatacgc agcgcaatca atacgccgcc caactcaagg tcagggactt caccattgag
    35881 catatgggca tacagaccgc gcgcagtatc ccggtctaaa tcgcgcgcgt ggtttttccc
    35941 gcgcccgatc tctttaatga ttttgcgata gtccatttac gactccttac ctgactcaca
    36001 tcattaacgc cgccgacggc gtggggattt cgttttttct ttctttttaa ctataccttc
    36061 agggacttcc ggttgctcta tcggaaatac cggtaatgca tccagtagcc gcttgccgta
    36121 gtttttggtg agcaagcgtt tatcgtaaat caccacttcg ccccagcaac cgtggcttcg
    36181 aatcagtcgc ccaacctgct gaatcaggtt aaacgaggcg ctcggcaggc tttgcacctc
    36241 aaacggatag cggttgaggc ttttcagcca ttccccttcg gtgatcacca cggggctgtc
    36301 gatgggcgga aaagcgattt tgtggatatg cacctggctg agcagatcgc ctttcagatc
    36361 aagcccttcg gcaaatgact gtaagcccac cagcacgctg cgctcaccgt tgccgacgcg
    36421 tttgcggtgc agttcaacta aacggtaacg cggctgatcg ccctgcacca gcaacatcag
    36481 gcgtaaatcc gtcacatagt cgagaaaccg ctgcatcgcc cgtccgctgg caaacagtac
    36541 caacataccg agatgttttt tgctctccac ctgctcacgg aaaaaggctg ccatttcagc
    36601 aatgtgctgc tcttcgttgt cgatggaagg ctcaaagcgc atccggggaa taacaatttt
    36661 gccctgttcg cagtggttaa agggcgaatc cagcgccaca aagcggtcgc ccgctttctc
    36721 tttcagacca ctcatctcct gcaaacgcga aaaactgttc agcgaacgta aggtcgcgga
    36781 agtgacaata atgtgcggaa tactgcgcca cagcagcctt tccagctgat cgctgacacg
    36841 aatacccaca cagtgaaacc agagatgcag ctgcccttcg cgctcttccc gcgtcgccca
    36901 tttggtcacc ggcgcaccgg aagattgcgc cagagacgcc agccgccaga gtttgctttg
    36961 cgcctcgaac atccccagcg cgcgattcat ctgcaaaatc aaccgatgca gacgtacaat
    37021 gtcatggctg ccggttttct cacttaaatc gttaagaaat aactccgcca ggccacgcag
    37081 catctcggtg agttttgcca gccgctggca gatctccagc acttcatctg gcagttcgcc
    37141 catcgcaaaa cggtgctctg cctcctgccc ggcaggcatg tagagattga gaatgttgtt
    37201 taacgaggcg ataagctcat acaactcttc acaatgcgca ttcaaacgtt cagggatcgc
    37261 cagcggcggg atggtcttcg ggcgaaactg ctccatgcag gtagcgacca gtttcgtgaa
    37321 caagtccaac tgtagtcgat accacggcgc ggtgatttcg gcactcatct ccagcgcatc
    37381 ccgcgccaca tctggcaggt gatgaccttc gtccagcacc agcagcaaat ttttcgggtc
    37441 aggcaatacg gcttcgcttt ccatcgccgc catcaccagc gcatggtttg ccaccaccac
    37501 ttccgcttcc tgaatctccc gacgagcgac aaaaaacggg cattcgcgat agtaataaca
    37561 gttgcggttg aggcagctgg ctttgtcggt acttaaacga cgccagagat cgtcatctat
    37621 agcgatatca gtatgatcgc gcagaccatc ccatttataa gtgtcgagat cgcccttcag
    37681 cttcgcacaa cgtttttgct cttcctgatt atttggcgtc agttcgtcgt caagaaacgc
    37741 cagcagatcc tgttgcgtgg gttcagtgct ggcgagcgcc gtcagattac gcgggcaaac
    37801 gtagcgccca cgcccaaaag cggcagtgaa tttaagatcg ggaatgatct ttttcagcag
    37861 cggtaaatct ttgctgtaga tctgatcctg caatgccacg ttggcggtac tcaccaccag
    37921 cgttttttgc tcttcgcggg caatggcgat gccgggaatc aaataagaga gcgttttccc
    37981 aacgccggtg ggggcttcaa tcgccagatg ccgcccttct tctccggcca gcgttttggc
    38041 gacgtccgca atcatctgcc gctgcggcgc acggggaata aagtcgggga tctgttcctg
    38101 aagcgcctta taccaggcgg caatttgcgc tttaagcgcg gcggttaatg ccatgagaaa
    38161 acctgaaata ctgtataaac agccaatatt gtggcatttt tcggttttca ggggtaatgg
    38221 tttttctgtt tctggaaagc ggctcgcaat aacgttttcc ggatgtgaag ttttctcgcg
    38281 gcagaagaaa catcattgtg cagcaaaaga cattgtacat cagcttattc cgctggcggc
    38341 cattgcagag cggtatgtaa aacacgcagt accgagagcg tatcgccctc cacttgatag
    38401 acaagcacat aatgcggcct gacaacggct tcatgtgtac cagcgactcg tccggcccgg
    38461 tacatctccg ggcgttcaca ggcctgttcc gcaatggctt caaattgatt atcaagggct
    38521 attgccgcta gcggattatc tttactgatg tattccatga tttgctccct gtcagacagg
    38581 gacattggct tccattccag acgcattaat cccccttcag caaggctttt ttacgggcag
    38641 caaatagact tttggcctct tcatgagaga gtgccgggcg tggatcgtca atggcggcct
    38701 gcacctgctc tcgaaaccag gcatcatacg cggcagcttc atgagctcgt tttaatgcct
    38761 cagccctgtc tggtcgggtc gttttcttct gaccgtccgg gtcgtaatta gaggcgtcga
    38821 cttcaaaccg ggcaatacca acatctttca ggtagttgac aaccgtttcc agccgtttga
    38881 acaggcggat ttgccgactg cgttgtgctg caagaggccg ttcagtgtgg ccgtatttca
    38941 cggtcaacga ccagcctccc tgggtgccaa taacgtgtgc tgcatccact acgccagatt
    39001 caaccagttt atgtaaggtt gagtgatcga tcgtttctgg tgccataatg cgctaacctc
    39061 tttgtataca gcatgcaatt tataattaaa gatagagcaa ttaataataa attgcagcac
    39121 cttgatcgta aattgcccag gataaaattg cggtagaaag agacttgttg cgaagatgcc
    39181 gggcatcgcc cggcataaaa gattactgcg cagcggcagg tttacgcgga cggcggcgac
    39241 gttgttgttc gcctgccggt ttggcatcgc cgagacgacg agacggtttt tctgcgggct
    39301 tcgcgttacc agacttcgca ccactttccg cacggcgtgg ttgctgttga ccgcgaccac
    39361 caccttgccc acgaccgccg ccgccacgtt gctggcgacc gttctggatc ggttcggctt
    39421 tgattgacgg gtccggctca taacccggaa tcgcaatgcg cgggatctct tttttcagca
    39481 gtttttcgat atcacgcagc agtttgtgtt catcaacaca caccagcgac aacgcttcac
    39541 cggtagcagc cgcacgaccg gtacgcccga tacggtggac ataatcttcc ggtacgtttg
    39601 gcagttcata gttgaccacg tgcggcagct cttcaatatc caggccgcgc gcagcgatgt
    39661 cagttgccac cagtacacga atatcgcccg atttaaaatc agccagcgca cgggtacgcg
    39721 caccttgcga tttattgccg tggatcgccg cactacggat gccatcttta ttgagctgtt
    39781 cagccagatg gttagcgcca tgtttggtac gggtaaacac cagcacctgc tgccagttcc
    39841 ctttcccaat catgtgcgac aacaattcgc gtttgcgttt cttatcaaca aagtgaacat
    39901 gctgggtcac ctgatcagac gcggtattgc ggcgtgccac ttcgatttcc agcgggttgt
    39961 gcaacagttt ttccgccaga gctttaatat cgtcagagaa ggtcgcggag aacaacaggt
    40021 tctggcgctt cgcaggtagt tttgttaaca cgcgacggat atcgtggata aagcccatgt
    40081 cgagcatgcg gtcagcttca tcgaggacga ggatttcaac ctgatccagc ttcaccgcat
    40141 tctgatgttc caggtccagc agacgtcccg gcgttgcaac cagcacatca acgccgccgc
    40201 gcaacttcat catctgcggg ttaatgctga caccgccaaa caccaccagc gaacgaatgt
    40261 tcaggtattt gctgtaatca cggacgtttt cgccaatctg cgccgccagt tcacgggtcg
    40321 gggtaagaat gagcgcacgt accggacgac gccctttggc gtgcggctgg cgagtgatca
    40381 ggtgttgcaa cagcggcagc gtaaagcccg ctgttttgcc ggtgccagtc tgggcgctag
    40441 ccatcaggtc gcggccttcc agcaccgcag ggatcgcctg ctgctgaata ggggtgggtt
    40501 cacggtaacc ctgctcggca acggcgcgca ggatatcagg gcttaaaccc aaagaatcga
    40561 aagacataac tactccgaac cgccccgacc gttaccggtg tagttttcag ggagatactg
    40621 aaaaggaaaa tgacaaaaac cacaatgtca tgagggagcg gagtgtagca gtttttgtga
    40681 cgcagcgcat aaattatccc tgtgactggc gttgctaaaa ttcttagcca tactggacaa
    40741 ctcccatatg gagtcataat cttaatcaat catttgatta agaatggact gtgcgatgaa
    40801 taatcctgcc atgacaatca agggtgaaca ggcgaaaaaa cagctgattg ctgccgcact
    40861 ggcgcagttt ggtgaatatg gaatgaacgc caccacccgc gagatagccg cccaggccgg
    40921 gcagaatatc gctgccatca cctactactt cggttcgaaa gaggatttgt acctcgcctg
    40981 cgcccagtgg attgccgatt ttattggcga gcagttccgt ccgcacgccg aagaggccga
    41041 acgcttgttc gcacaaccac agccagatcg ggctgccatc cgtgaactga tccttcgcgc
    41101 ctgcaggaac atgattaagc tgctcaccca ggatgacact gtcaacctca gcaaatttat
    41161 ctcccgtgag cagctctctc ccacggcagc ctatcacctg gtgcacgaac aggtgattag
    41221 tccgctacac agccacctta cacgcctgat tgccgcctgg accggctgcg acgccaatga
    41281 tacccgcatg atccttcata cccatgcgtt gattggtgag attctggcgt ttcgtcttgg
    41341 caaagaaaca attctgttac gtaccggctg gaccgcgttc gatgaagaaa agaccgaact
    41401 gattaaccag acggtgacct gtcatatcga cctgattttg caaggattat cgcaaaggag
    41461 tttgtagtga tgaaaaaacc tgtcgtgatc ggactggcgg tagtagtact tgccgccgtg
    41521 gttgccggag gctactggtg gtatcaaagc cgccaggata acggcctgac gctgtatggc
    41581 aacgtggata ttcgtacggt gaatcttagt ttccgtgttg gggggcgcgt tgagtcgctg
    41641 gcagtggacg aaggtgatgc gatcaaagcg ggccaggtgc tgggcgaact ggatcacaag
    41701 ccgtatgaga ttgccctgat gcaggcgaaa gcgggcgttt cggtggctca ggcgcagtat
    41761 gacctgatgc ttgccgggta tcgcgatgaa gaaatcgctc aggccgccgc agcggtgaaa
    41821 caggcgcaag ctgcctatga ctatgcgcag aacttctata accgccagca aggactgtgg
    41881 aaaagccgca ccatttcggc aaatgacctg gaaaatgccc gctcctcgcg cgaccaggcg
    41941 caggcaacgc tgaaatcggc acaggataaa ttgcgtcagt accgttccgg taaccgcgaa
    42001 caggatatcg cccaggcaaa agccagcctc gaacaggcgc aggcgcaact ggcgcaggcg
    42061 gagctgaatt tgcaggactc aacgttgata gccccgtctg atggcacgct gttaacgcgc
    42121 gcggtggagc caggcacggt tctcaatgaa ggtggcacgg tgtttaccgt ctcgctaaca
    42181 cgtccagtgt gggtgcgtgc ctatgttgac gaacgtaatc ttgaccaggc ccagccgggg
    42241 cgcaaagtgc tgctttatac cgatggtcgc ccggacaagc cgtatcacgg gcagattggt
    42301 ttcgtttcac cgactgctga atttaccccg aaaaccgtcg aaacgccgga tctgcgtacc
    42361 gacctcgtct atcgcctgcg tattgtggtg accgacgccg atgatgcgtt acgccaggga
    42421 atgccagtga cggtacaatt cggtgacgag gcaggacatg aatgatgccg ttatcacgct
    42481 gaacggcctg gaaaaacgct ttccgggcat gaacaagccc gccgtcgcgc cgctcgattg
    42541 caccattcat gccggttatg tgacggggct ggtagggcca gacggtgcgg gtaaaaccac
    42601 gctgatgcgg atgctggcgg gattactgaa acccgacagc ggcagtgcca cggtgattgg
    42661 ctttgatccg atcaaaaatg acggcgcgct gcacgccgtg ctcggctata tgccgcagaa
    42721 atttggtctg tatgaagatc tgacggtgat ggagaacctc aatctttacg cggatttgcg
    42781 cagcgtcacc ggcgaggcac ggaagcagac tttttctcgc ctgctggagt ttacgtctct
    42841 tggcccgttt accggacgcc tggcgggcaa gctctccggt gggatgaaac aaaaactcgg
    42901 cctggcctgt accctggtgg gcgaaccgaa agtgttgctg ctcgatgaac ccggcgtcgg
    42961 cgttgaccct atctcacggc gcgaactgtg gcagatggtg catgagctgg cgggcgaagg
    43021 gatgttaatt ctctggagca cctcgtatct cgacgaagcc gagcagtgcc gtgacgtgtt
    43081 actgatgaac gaaggcgaat tgttgtacca gggagaaccg acggccctga cacaaaccat
    43141 ggccggacgc agctttctga tgaccagccc gcacgagggc aaccgcaaac tgttgcaacg
    43201 ggcattgaaa ctgccgcaag tcagcgacgg catgattcag gggaaatcgg tacgtctgat
    43261 cctcaaaaaa gaggccacac cagacgatat tcgccatgcc gacgggatgc cggaaatcaa
    43321 catcaacgaa actacgccgc gttttgaaga tgcgtttatt gatttgctgg gcggtgccgg
    43381 aacctcggaa tcgccgctgg gtgcaatatt gcatacggta gaaggcacac ccggtgagac
    43441 ggtgatcgaa gcgaaagaac tgaccaagaa atttggtgat tttgccgcca ccgatcacgt
    43501 caactttgcc gttaaacgcg gggagatttt tggtttgctg gggccaaacg gcgcgggcaa
    43561 atcgaccacc tttaagatga tgtgcggttt gctggtaccg acctctggcc aggcgctggt
    43621 gctgggcatg gatctgaaag agagttccgg taaagcgcgc cagcatctcg gctatatggc
    43681 gcaaaaattt tcgctctacg gcaacctgac ggtcgaacag aatttacgct ttttctctgg
    43741 tgtgtatggc ttacgcggtc gggcgcagaa cgaaaaaatc tcccgtatga gcgaggcgtt
    43801 cggcctgaaa agtatcgcct cccacgcgac cgatgaactg ccattaggtt ttaaacagcg
    43861 gctggcactg gcctgctcgc tgatgcatga accggacatt ctgtttctcg acgaaccgac
    43921 gtccggcgtt gatcccatca cccgccgtga attttggcta catatcaaca gtatggtaga
    43981 gaaaggcgtc acggtgatgg tcaccaccca ctttatggat gaagcggaat attgcgaccg
    44041 catcggcctg gtgtaccgcg ggaaattaat cgccagcggc acaccggacg atttgaaagc
    44101 gcagtcggcc aacgatgagc aacccgatcc cactatggag caagccttta ttcagttgat
    44161 ccacgactgg gataaggagc atagcaatga gtaatccgat cctgtcctgg cgtcgcgtgc
    44221 gggcgctgtg cgtcaaagag acacggcaga tcgttcgcga tccgagtagc tggctgattg
    44281 cggtagtgat ccctctgctg ctgctgttta tttttggcta cggcattaac ctcgactcca
    44341 gcaagctgcg ggtcgggata ttgctggaac agcgtagcga agcggcgctg gatttcaccc
    44401 acaccatgac aggttcgccc tacatcgacg ccaccatcag cgataaccgt caggaactga
    44461 tcgccaaaat gcaggcgggg aaaattcgcg gtctggtggt tattccggtg gatttcgccg
    44521 aacagatgga gcgcgccaac gccaccgcac cgattcaggt gatcaccgac ggcagtgagc
    44581 cgaataccgc taactttgtg caggggtatg tcgaagggat ctggcagatc tggcaaatgc
    44641 agcgagcgga ggacaacggg cagacttttg aaccgcttat tgatgtacaa acccgctact
    44701 ggtttaaccc ggcggcgatt agccagcact tcattatccc cggtgcggtg accatcatca
    44761 tgacggtcat cggcgcgatt ctcacctcgc tggtggtggc gcgagaatgg gaacgcggca
    44821 ccatggaggc tctgctctct acggagatta cccgcacgga actgctgctg tgtaagctca
    44881 taccctacta tttcctcggg atgctggcga tgttgctgtg tatgctggtg tcggtgttta
    44941 ttctcggcgt gccgtatcgc gggtcgctgc tgattctgtt ttttatctcc agcctgtttt
    45001 tactcagtac cctggggatg gggctgctta tttccacgat tacccgcaac cagttcaatg
    45061 ccgctcaggt cgccctgaac gcggcttttc tgccgtcgat tatgctttcc ggctttattt
    45121 ttcagatcga cagtatgccc gcggtgatcc gcgcggtgac gtacattatt cccgcacgtt
    45181 atttcgtcag caccctgcaa agcctgtttc tcgccgggaa tattccggtg gtgctggtgg
    45241 tgaacgtact gtttttgatc gcttcggcgg tgatgtttat cggcctgacg tggctgaaaa
    45301 ccaaacgtcg gctggattag ggagaagagc atgtttcatc gcttatggac gttaatccgc
    45361 aaagagttgc agtcgctgct gcgcgaaccg caaacccgtg cgattcttat tttacccgtg
    45421 ctgattcagg tgatcctgtt cccgttcgcc gccacgctgg aagtgactaa cgccaccatc
    45481 gccatctacg atgaagataa cggcgagcat tcggtggagc tgacccaacg ttttgcccgc
    45541 gccagcgcct ttactcatgt gctgctgctg aaaagcccac aggagatccg cccaaccatc
    45601 gacacacaaa aggcgttact actggtgcgt ttcccggctg acttctcgcg caaactggat
    45661 accttccaga ccgcgccgtt gcagttgatc ctcgacgggc gtaactccaa cagtgcgcaa
    45721 attgccgcca actacctgca acagatcgtc aaaaattatc agcaggagct gctggaagga
    45781 aaaccgaaac ctaacaacag cgagctggtg gtacgcaact ggtataaccc gaatctcgac
    45841 tacaaatggt ttgtggtgcc gtcgctgatc gccatgatca ccactatcgg cgtaatgatc
    45901 gtcacttcac tttctgttgc ccgcgaacgt gaacaaggta cgctcgatca gctactggtt
    45961 tcgccgctca ccacctggca gatcttcatc ggcaaggccg taccggcatt aattgtcgcc
    46021 accttccagg ccaccattgt gctggcgatt ggtatctggg cgtatcaaat ccccttcgcc
    46081 ggatcgctgg cgctgttcta ctttacgatg gtgatttacg gtttatcgct ggtgggattc
    46141 ggtctgttga tttcatcact ctgctcgaca caacagcagg cgtttatcgg cgtgtttgtt
    46201 tttatgatgc ccgccattct cctttccggt tacgtttctc cggtggaaaa catgccagta
    46261 tggctgcaaa acctgacgtg gattaaccct attcgccact ttacggacat taccaagcag
    46321 atttatttga aggatgcgag tctggatatt gtgtggaata gtttgtggcc gctactggtg
    46381 ataacggcca cgacagggtc agcggcgtac gcgatgttta gacgtaaggt gatgtaactt
    46441 cttatctttc gccagcaaag acaccaccgc cgggccggca aggattgcca gccctgcaat
    46501 cgccagcaaa aagttgtttt ccagtacacg ggacaatagc cacagcacaa tcagcgctgc
    46561 gccaaaatac caggtcgtgg tcagctcttc cagcacgtct gccacctcac gccagcgttg
    46621 ctcggggtgg cgctgaaaca ccagcatcat cactaccgtg acacaataca gtgcgcataa
    46681 tcccacgccg acgtgaacca gagtcttact catccagccc accaccagca gcgccactac
    46741 cagtaaatgc aacataatct gccagcaact tagacccgtt gcgacacgaa cacgttgttg
    46801 ccacttcatg gcttctctcc tgaagaattt atctctactt actcagagta ccgaacttta
    46861 cggaaaattc cgtaagaccg cacctttttg tgacgccgac tacactattt caatgagata
    46921 agaggcaaag acaggaaaat tatgcccgat caaacacaac aattttcgtt caaggtgctc
    46981 accatcaata ttcacaaagg ctttaccgcg tttaaccgac gcttcatttt gccggaactt
    47041 cgcgacgccg tgcgtaccgt cagcgccgat attgtttgcc tgcaggaagt gatgggcgcg
    47101 cacgaagttc atccgctgca tgtggaaaac tggcccgata cctcgcacta cgagtttctc
    47161 gccgacacta tgtggagcga ttttgcctac ggtcgcaatg ccgtataccc ggaagggcat
    47221 cacggtaacg ccgtactgtc gcgctatccc attgaacatt atgaaaaccg cgatgtttcg
    47281 gtcgatggtg cggaaaagcg cggcgtgctc tactgccgca ttgtgccgcc gatgaccgga
    47341 aaagcgattc atgtaatgtg cgtacatttg ggcctgcgtg aggcgcaccg tcaggcgcag
    47401 cttgcgatgc tcgccgaatg ggtgaatgag ctaccggacg gcgaaccggt attggtggcg
    47461 ggtgatttca atgactggcg gcaaaaagct aatcatccgt taaaagtgca ggccggactg
    47521 gatgagattt ttacccgcgc ccacggacgc ccggcgcgca cgtttccggt gcaatttcct
    47581 ctactacgac tggacaggat ctacgtcaaa aatgccagcg ccagcgcgcc aaccgcgttg
    47641 ccgctgcgga catggcgaca cctttctgat catgcccctt taagtgcgga gattcattta
    47701 tgaaatgtag ctggcgcgaa ggcaataaga tccagttgct ggaaaacggc gagcaatatt
    47761 atcccgcggt gtttaaggcg attggcgagg cacaagaacg catcattctt gaaacgttta
    47821 tctggtttga ggatgacgtc ggcaaacaac tgcatgcggc actactggca gcagcgcaac
    47881 gcggggtaaa agcggaagtc ttacttgatg gctacggttc gccggatctc agcgatgagt
    47941 ttgtcaatga actgacggcc gctggcgtgg tgttccgcta ctacgatccc cgccctcgcc
    48001 tttttggtat gcgcaccaat gtgtttcgcc ggatgcatcg caaaattgtg gtgatcgacg
    48061 cgcatatcgc ctttattggc gggctgaatt actccgccga gcatatgtcc agctacggtc
    48121 cagaggctaa acaggattac gcggtgcgcc ttgaagggcc gattgtcgaa gatattctgc
    48181 aatttgaact ggaaaacctg ccaggacaga gcgcggcacg acgctggtgg cgacgtcatc
    48241 acaaagcgga agagaaccgc cagccgggag aagcgcaggt attgctggtc tggcgcgata
    48301 acgaagaaca tcgcgatgat attgaacgtc actatctgaa aatgctcact caggcacggc
    48361 gggaagtgat tatcgccaac gcctacttct tccccggcta tcgattttta cacgccttgc
    48421 gtaaagcggc acggcgcggg gtgcggatca aactgatcat tcagggcgaa ccggatatgc
    48481 cgattgtcag agtcggcgcg cgcttgctgt ataactatct ggttaaaggc ggcgttcagg
    48541 tttttgagta tcgccgccgc ccgctccacg gcaaagtggc gttgatggac gatcactggg
    48601 cgacggtagg gtccagtaat ctcgatccgc tcagtttgtc actgaatctc gaagcaaatg
    48661 tcatcatcca cgatcgtcat tttaaccaga cgctgcgcga taatctgaac ggcattatcg
    48721 ccgcagactg ccagcaggtg gatgaaacca tgctgccgaa acgcacctgg tggaatctga
    48781 ccaaaagcgt gctggccttc cactttttac gccacttccc ggcgctggtc ggctggcttc
    48841 cggcacacac accacgtctg gcgcaggttg acccgcccgc tcagccgaca atggaaacgc
    48901 aggatcgggt agaaactgaa aacacggggg taaaaccctg atgagtaaat cacacccacg
    48961 ctggcgctta gcaaagaaga tcctcacctg gctgtttttt atcgcggtga tagtgttgct
    49021 ggtggtctac gccaaaaagg tggactggga agaggtctgg aaggtcatcc gcgactacaa
    49081 tcgcgttgcg ttgctcagtg cggtcgggct ggtggtcgtc agttatctga tttacggctg
    49141 ctatgatctg ctcgcccgct tttactgcgg tcacaaactg gcgaagcgcc aggtgatgct
    49201 ggtgtcgttt atctgctacg ccttcaacct gacgctcagt acctgggtcg gcggcattgg
    49261 tatgcgctat cgtttgtact cccggctagg gttaccgggc agcactatta cgcggatttt
    49321 ctcgctcagt attaccacca actggctggg ctatatttta ctggcaggga ttatctttac
    49381 cgcaggcgtg gtggagttgc cggatcactg gtatgtcgat caaaccacgc tgcgtattct
    49441 cggcattggc ttattgatga ttatcgcggt ttatttgtgg ttttgcgctt tcgcgaagca
    49501 ccgccatatg accatcaaag gacaaaagct ggtgctgcct tcatggaaat ttgccctcgc
    49561 ccagatgctg atttccagcg ttaactggat ggtaatgggg gcgattatct ggctgttact
    49621 tggtcaaagc gtgaactatt tctttgtact gggcgtgtta ctggttagta gtattgctgg
    49681 cgtcatcgtg catattccgg cggggattgg tgtgctggaa gcggtattta tcgcgctact
    49741 ggctggggag catacctcga aagggagcat tatcgccgcc ctgctcgctt atcgcgtcct
    49801 gtattacttt atcccgctgc tgctggcgct gatctgttac ctgctactgg aaagccaggc
    49861 gaagaagcta cgagcgaaaa atgaagcggc gatgtgaagt ttagttaagt tctttagtat
    49921 gtgcatttac gactaatgaa aaaaacgcgt atgcctttgc cagacaagcg ttatagctat
    49981 cgcggcaagt atcccaaaga agccgataaa atagtaatag agtgttaatg cacctgtaaa
    50041 agcgcaacga accggactcc acaactcttc gctatggcat tttcctgcca gttcaaggag
    50101 cttatttcgt ccatgcgaga taatgcctga ccataacact atcattaagc aacttattac
    50161 ccaaacgggt cgttcactca ttaatgcggc atccatgatg atcaccaggg ccagctctgc
    50221 ggttgccacc atacccaaag cggtaatgag ccatataccg tttaatatcc tggcctcgtg
    50281 ctgaaaatct aatttattgc tgttttgcga gtatgattcc attcttttag ttgtccttag
    50341 aaaaaacatt aatattcacc atcaataaat ttaaaaccac gcatacaaaa tatttttgta
    50401 tttatggata cggaaaacac ttccgctcgg ccataaggca tattttatac ctggtacttg
    50461 ttaagtgtcg tttttagtaa gaaaaaaatc ccgacacatg gccgggatat tgatggtgaa
    50521 aaagaattaa cggcggttgc cgaagatccg caacaacatc aggaacaagt tgatgaagtc
    50581 cagatacaag gttaacgcgc caagaatgga atatttgcgc aggttcgacg tgtcgcgggt
    50641 atcaatctgc tcacccatat ttttcaattt ctgagtgtca tacgccgtca atccgacaaa
    50701 gacaatcacg ccgatgtagg taactgccca catcaatgct tcgcttttca gccagaagtt
    50761 gaccagcgat gccagcacaa tgccgattaa cgccataaac agcatattgc caaagccgct
    50821 tagatcgcgc ttcgtggtgt aaccgtacag gctcattgcg ccaaacatcc cggcagtaac
    50881 gacgaaagta ctggcgatag aagcagcggt atagacaatg aaaatactgg aaagcgtaag
    50941 acccgtcagc gccgaataaa gcataaagag catcgtcgtt acacctgcgc tcagcttttg
    51001 aatcatcgct gataacacaa taaccaatgc taattgcgcg atgatcagac cgattaaaaa
    51061 gacacggtta gtgaacaaca gctccatcac ggccgcggaa ttagccgcat accaggcaac
    51121 aaatgcggtc agcaacaagc caacggtcat ccagccatag acttgcgcca tataagtttg
    51181 caagccagcc cggggttgta caattgaatc agaacgtggg aatctgtcca tgacgatctc
    51241 ctgaagatat aaggaatatc ttaaggatac tgcaaaatga tgaggctgtg catcgacgca
    51301 gcgtaaacgc atgtactgag cggtgaaatt gccggacgca gcggtgcctt atccggctaa
    51361 caaaaaacta ccagcgtttt gccgcctgct gatcgctctc ccgagcttcg acccagcggt
    51421 cgccttccgg cgtggcttcg cgcttccaga acggtgcgcg ggttttgaga taatccataa
    51481 taaactgccc ggcttcaaac gcactgctgc gatgcgcact ggtgacaccg acaaaaacga
    51541 tttcatcgcc cggccataat tccccgatgc ggtgaatcac cgtgacgcgc cccagcggcc
    51601 agcggttacg cgcttcatcg acaatttctg ccagtgcttt ttcagtcatc cccggatagt
    51661 gttcgagggt taatgctttg acgctgtcgc caaggttatg gttgcgcacc ttaccggtaa
    51721 aggtgactac cgcaccgtct tcatcacgct ccgccagcca cggatactct tctcctacgc
    51781 tgaatggctg cgggccgaca acaattttgg tttctgccat cttaacctcc ggttaccggc
    51841 gggaagaaag ccacttcgtc gccgtcagtc agcggatggt caagactcac cagcgtctgg
    51901 ttgacggcag ccagcaattt gccatcttcc agcgccagcg cccagcgatc gctctgcgca
    51961 gccagatgtt ggcgtaacgc ttcaacggtt gggaaatccg cagccacttc ggttgcatct
    52021 gttcccacca actcgcgcac ctgggcgaaa aaaagaactt taatcatccg cttccacctt
    52081 aaagtcaccc gacttgccgc cgctcttcgc cagcaaacgt accggaccaa ttaccatatc
    52141 tttttgcacc gctttgcaca tgtcataaat ggtcagcgcc gccacggagg ccgcggttaa
    52201 cgcttccatt tcgacaccgg ttttcccggt caggcggcat aaggtttcta tacgtacccg
    52261 attgtgctcc ggctcggcct gtaaattgac ttcaactttg ctgagcatca gcgggtgaca
    52321 cagcgggatc agatcccagg tgcgttttgc cgcctgaata ccggcgatac gcgcagtggc
    52381 aaatacgtcg cctttgtggt ggcgaccatc aataatcatc gccagcgtct cgctgcgcat
    52441 ggtgacaaag gcttccgccc gcgcttcacg cacggtttcc gctttggcgg agacatccac
    52501 catgtgtgct tcgccagcgg cgttgatatg ggtcagttgc gacatactta tttcttcaaa
    52561 tgtggatgga aattgcatgg acgtgtacgg gcatcgagct gcggcgcgat gatattttcc
    52621 cacgcggtgc ggcacgcttt ggtcgaaccc ggcatggcga aaatcagcgt tttgttagcg
    52681 acgcccgcta ccgcacgaga ttgcaacgtt gaggtgccaa tctcttcaaa cgacaacatc
    52741 cggaacactt caccaaaacc ttcaacttca cggtcgaaca acggcagcag cgcttcggga
    52801 gcctgatcac cttccgtcag gccagtacca cccgtaatca ataccacttg tacatcgtcg
    52861 ctggcgatcc acgcagatac ctgagcgcga atagcgtagc ggttttcttt cacaatggct
    52921 ttatcgacaa cgtgatggcc cgcttcctgc gccgaatcgc gcagatagtg accggaggta
    52981 tcgtcttctt caccacgacg attagaaacc gtaagaatag caatacgggt cgggataaat
    53041 tcagtgctta cctgactcat ctgatctctc cttttgacgt tttagccgcc aatgtacgat
    53101 aagttttgcg taataccggt gttgttttga tgcaggaaat gggtctgttt cttctcccgc
    53161 agcgccgctg aaatacgcgc ttccagcgcc tgttgctggg tatcgtcttc cagcagatcg
    53221 cgcaggttaa cgccgccttc accaaacagg cagagatgga gtttaccaat ggaggaaacg
    53281 cgcaggcggt tgcaagtggc gcagaagtct ttttcatacg gcatgataag gccaatctct
    53341 ccggcgtaat ccggatgaca aaagacttgc gcgggaccgt cgctgcgttg acgtaattgg
    53401 tggatccagc cgcgacgcag tagctcgtca cgcagaacct gaccagagat gtgatgctta
    53461 cggaagagct cgctgccctc acccgtttcc atcaattcga tgaaacgcag ctggataggg
    53521 cgatgctgga tccagttcag aaaggtgtcg agctgatgat gattaacatc acgcatcagc
    53581 acggtattga ctttgacctt ttcaaaaccg gcctcaaatg cggcatcgat ccctgccatg
    53641 acctggttga atttatcctg cccggtaatg gcatgaaact ggtgagcgtc aaggctatca
    53701 acgctgacgt tgatgccagt aagtccagca tcgcgccagt tcgccacatc gcgttccaga
    53761 cggtaaccat tggtggtgac cgcaatctgg cggatagcgt cgttttcccg cacagcggcg
    53821 atgatatcgg taaagtcgcg gcgtaaagac ggctcaccac ctgtcagacg gactttttcg
    53881 gtgccaagac tggcgaaggc gcgcgtaacc cggcgaattt catcgacggt aagaaagcct
    53941 ttattggtga cgccgctcgg tttgtagcca tccggcaggc agtaggtgca acgaaagtta
    54001 cacacatcgg taatcgacag gcgcaagtag taaaacttac gcgcaaatgc atcagtcagt
    54061 tgtgaagcca tgtacacctt tccagatacg ggaggcgaag tcatttcttc cttcgccctg
    54121 gtggcaatct ttacgagcat tgtcacggcc aaagcaccgt atcagttgac ccaggtgcag
    54181 aggctagagt gtttagtggt taagccgata ctagcgtgta aatgagattt ttaccatcca
    54241 cactttcgct atgtaatcat gtatatagcg tcatgatcgt gctatttcgc tacagaataa
    54301 gggaaaatgg cggttttact ttgatataca tcatcaggaa ggcatagcgt acatcgtcct
    54361 ttagaggtag aagaaaatag tttgtatcct caatattggc aggttaattg ctgtttcccc
    54421 gcaatttgcg ctactgtagc gcggataatc tgactccagg aaactatatg cgcaatcgta
    54481 cgctggctga ccttgatcgt gtcgttgctc tcggcggagg gcatggactg ggacgcgttc
    54541 tctcatcact ttcgtctttg ggttctcgtt taacgggtat cgtcaccacc acggataatg
    54601 gtggctcgac ggggcgtatt cgccgctcag aaggcggtat tgcctggggc gatatgcgta
    54661 actgcctcaa ccagctgata actgaaccca gcgtcgcttc cgcaatgttt gaatatcgtt
    54721 tcggcggtaa tggcgaactt tccggacaca accttggaaa cctgatgctg aaggcactgg
    54781 atcaccttag cgtgcgacct cttgaagcga tcaatctgat tcgcaatctg ctgaaagtgg
    54841 acgcacattt gattccgatg tcggagcatc ctgttgatct gatggcgatt gacgatcagg
    54901 ggcatgaagt ttacggcgag gtcaatatcg accagttaac tgcgccgatt caagagttat
    54961 tgttaacgcc gaatgtaccc gcaacccgtg aggcggttca cgctatcagt gaagcggatc
    55021 tcatcattat tgggcctggc agtttttaca ccagcctgat gccaattctg ctgctgaatg
    55081 aaatcgcaca agcattacgc cgcacgccag cgccgatggt ctatatcggc aatctggggc
    55141 gtgagttaag tttacctgcg gctaatctga agctggaaag caagctggca attatggagc
    55201 agtatgttgg taaaaaggtc attgatgcgg tcatcgtcgg gcccaaagtg gatgtctcgg
    55261 cggtgaaaga gcggattgtg attcaggagg tgctggaggc cagcgatatt ccgtatcgtc
    55321 atgaccgcca gttgctgcat agcgcgctgg aaaaggcatt acaggcactg ggttaatgtg
    55381 gccgagattg ctgaccaggc ttctggtgca atgcgacctg ttcccctcac cctaaccctc
    55441 tctccggagg ggcgagggga ccgttcgagc acttttttga cttgatcgtt aatctgagtc
    55501 ggaagtacat tccggctttt tcatcttgtc acgacgcggc aataaacagc tcacgcagct
    55561 gatgcaactg gtcacgaatt tgcgccgctt cttcgaactc cagattctgc gcgtgttgca
    55621 tcatcaaccc ttccagttca tggatcttct gctgcaacgc tttaggcgac atatccatcg
    55681 gcacattatc cggctcaaca atcgggcgcg attttcctct gcccttcgct ttggttttgg
    55741 caatgttctg ccccagcgcc aggatatcga ccactttctt gttcaagcct tgcggcgtaa
    55801 tgccgtgttc ctcgttgtac ttctgctgtt tctcgcggcg acgttcggtt tcgccaatcg
    55861 ctttcgccat tgatggggtg atcttatcgc cgtagagaat cgctttaccg ttaacgttac
    55921 gtgccgcacg accaatggtc tggatcaacg aacgttcgga acgcaggaag ccttctttgt
    55981 cagcgtcgag gatcgccacc agcgacactt ccggcatatc cagaccttcg cgcagtaagt
    56041 tgatccctac caatacgtcg aactcaccca gacgcaagtc gcggataatc tccatacgtt
    56101 cgacggtgtc gatatctgag tgaagataac gcacgcgctc accgtgttct tcgagatatt
    56161 cagtgagatc ttccgccatc cgcttggtca gagttgtaac cagtacgcgt tcgttaattg
    56221 ccgctcgctg acgaatctcc gaaagaagat catccacctg tgttgctacc ggccgcactt
    56281 cgataatcgg gtcgagtaag cctgttggac gcaccacctg atccaccaca tcaccaccgg
    56341 atttttccag ctcgtaatta cccggcgtcg ccgaaacata gatggtttgc ggcgctaatg
    56401 cttcgaactc ttcaaatttc agcggacggt tatccagcgc tgatggcagg cggaagccgt
    56461 actccaccag agtctcttta cgcgcccggt caccgcgata catgccacca atttgtggaa
    56521 tggtgacgtg agattcgtcg acgaccagca gcccatcggc aggcaggtaa tcaaacagcg
    56581 tcggcggtgg ctcacccggt ccacgaccgg agaggaagcg cgagtagttt tcaatccccg
    56641 aacagtagcc cagctcgttc atcatctcca gatcaaactg ggtacgctgg gtcagccgct
    56701 gctcttccag cagtttgttg ttttccaaca gcactttgcg tctggcggcc agttcttctt
    56761 tgatctcctc catcgcctgt acgatgcgct cacgtggcgt gacgtagtgc gttttcgggt
    56821 agatggtaaa acgtggaata gtggaaacaa tctgcccggt tagcgggtca aataacgaca
    56881 atcgttccac ttcctcgtca aacagttcca cgcgaagtgc aatgtcatcc gattctgccg
    56941 ggaagatatc tatcacctca ccacgaacgc ggaaagtacc gcgctggaat gcctgatcat
    57001 tacgagcgta ttgcagctcc gccagtcggc gcaggatcgc gcgctgatcg ataatcatgc
    57061 cgaccgtgag atggagcatc atcttgagat ataaatcagg atcgcccaga ccataaatcg
    57121 cggaaacaga cgccaccaca accacatcac gccgctccag catcgctttg gtagcggaca
    57181 aacgcatctg ctcaatatgt tcgtttaccg aggcatcttt ctcaatgaag gtgtcggaac
    57241 tcggtacata ggcttccggc tgatagtagt cgtagtagga gacgaaatat tccaccgcgt
    57301 tttccgggaa gaactctttc atttcgccgt acagttgggc cgccagcgtt ttgttgggcg
    57361 ccagtaccat ggttgggcgc tgaaggtcag caatgacatt ggcgatggtg aaggttttcc
    57421 cggagccagt tacaccgagt aacgtctggt gcgccaggcc atcttccagc ccctcttcga
    57481 gacgtcgaat cgcctctggc tgatcgccag aaggtttaaa agcggaattc agtttgaacg
    57541 gtttactcat gagtcgctac ctgaaggagt tgggcgggca ggtatgtaat tttact
//
LOCUS       DACBVI010000013        53375 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.013, whole genome shotgun sequence.
ACCESSION   DACBVI010000013 DACBVI010000000
VERSION     DACBVI010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.013"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(309..1202)
                     /gene="galF"
                     /locus_tag="IU325_04075"
     CDS             complement(309..1202)
                     /gene="galF"
                     /locus_tag="IU325_04075"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HAP5808217.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(1445..2440)
                     /locus_tag="IU325_04080"
     CDS             complement(1445..2440)
                     /locus_tag="IU325_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HAP5808218.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            complement(2598..3992)
                     /gene="wcaM"
                     /locus_tag="IU325_04085"
     CDS             complement(2598..3992)
                     /gene="wcaM"
                     /locus_tag="IU325_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HAP5808219.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFGEGQTVVVPAGLVCDNINTGIFIPAGKTLHILGSLRGNGRGRFVLQDSS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLTMSGFGPVTQIYIGGKNKRVMRNLSIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQMDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRVNHHIV
                     NVEKINFRLPERRE"
     gene            complement(4003..5223)
                     /gene="wcaL"
                     /locus_tag="IU325_04090"
     CDS             complement(4003..5223)
                     /gene="wcaL"
                     /locus_tag="IU325_04090"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HAP5808220.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIIALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSRDV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSITGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLNTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(5220..6500)
                     /gene="wcaK"
                     /locus_tag="IU325_04095"
     CDS             complement(5220..6500)
                     /gene="wcaK"
                     /locus_tag="IU325_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HAP5808221.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(6872..8350)
                     /gene="wzxC"
                     /locus_tag="IU325_04100"
     CDS             complement(6872..8350)
                     /gene="wzxC"
                     /locus_tag="IU325_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M-antigen undecaprenyl disphosphate flippase"
                     /protein_id="HAP5808222.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDAI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(8352..9746)
                     /gene="wcaJ"
                     /locus_tag="IU325_04105"
     CDS             complement(8352..9746)
                     /gene="wcaJ"
                     /locus_tag="IU325_04105"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HAP5808223.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(9801..11171)
                     /gene="cpsG"
                     /locus_tag="IU325_04110"
     CDS             complement(9801..11171)
                     /gene="cpsG"
                     /locus_tag="IU325_04110"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HAP5808224.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLRDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(11364..12800)
                     /gene="cpsB"
                     /locus_tag="IU325_04115"
     CDS             complement(11364..12800)
                     /gene="cpsB"
                     /locus_tag="IU325_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HAP5808225.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDMVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(12803..14026)
                     /gene="wcaI"
                     /locus_tag="IU325_04120"
     CDS             complement(12803..14026)
                     /gene="wcaI"
                     /locus_tag="IU325_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HAP5808226.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNIIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKYNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(14023..14502)
                     /locus_tag="IU325_04125"
     CDS             complement(14023..14502)
                     /locus_tag="IU325_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HAP5808227.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            complement(14505..15470)
                     /gene="fcl"
                     /locus_tag="IU325_04130"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(14505..15470)
                     /gene="fcl"
                     /locus_tag="IU325_04130"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAP5808228.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(15473..16594)
                     /gene="gmd"
                     /locus_tag="IU325_04135"
     CDS             complement(15473..16594)
                     /gene="gmd"
                     /locus_tag="IU325_04135"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAP5808229.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(16621..17169)
                     /gene="wcaF"
                     /locus_tag="IU325_04140"
     CDS             complement(16621..17169)
                     /gene="wcaF"
                     /locus_tag="IU325_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HAP5808230.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATLFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDEVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(17185..17931)
                     /gene="wcaE"
                     /locus_tag="IU325_04145"
     CDS             complement(17185..17931)
                     /gene="wcaE"
                     /locus_tag="IU325_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HAP5808231.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNSIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAAYF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            complement(17942..19159)
                     /gene="wcaD"
                     /locus_tag="IU325_04150"
     CDS             complement(17942..19159)
                     /gene="wcaD"
                     /locus_tag="IU325_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HAP5808232.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAMFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(19134..20351)
                     /gene="wcaC"
                     /locus_tag="IU325_04155"
     CDS             complement(19134..20351)
                     /gene="wcaC"
                     /locus_tag="IU325_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HAP5808233.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDITLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGRPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFIQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(20348..20836)
                     /gene="wcaB"
                     /locus_tag="IU325_04160"
     CDS             complement(20348..20836)
                     /gene="wcaB"
                     /locus_tag="IU325_04160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HAP5808234.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(20839..21678)
                     /gene="wcaA"
                     /locus_tag="IU325_04165"
     CDS             complement(20839..21678)
                     /gene="wcaA"
                     /locus_tag="IU325_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HAP5808235.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYNNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(21771..23933)
                     /gene="wzc"
                     /locus_tag="IU325_04170"
     CDS             complement(21771..23933)
                     /gene="wzc"
                     /locus_tag="IU325_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HAP5808236.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLTKEGVTLMVEAIHASPGSEFTITKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(23936..24379)
                     /gene="wzb"
                     /locus_tag="IU325_04175"
     CDS             complement(23936..24379)
                     /gene="wzb"
                     /locus_tag="IU325_04175"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HAP5808237.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(24385..25524)
                     /locus_tag="IU325_04180"
     CDS             complement(24385..25524)
                     /locus_tag="IU325_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="required for the translocation of capsular
                     polysaccharide through the outer membrane; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HAP5808238.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            26183..27766
                     /locus_tag="IU325_04185"
     CDS             26183..27766
                     /locus_tag="IU325_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP5808239.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLIMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(28040..29893)
                     /gene="asmA"
                     /locus_tag="IU325_04190"
     CDS             complement(28040..29893)
                     /gene="asmA"
                     /locus_tag="IU325_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HAP5808240.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(29915..30496)
                     /locus_tag="IU325_04195"
     CDS             complement(29915..30496)
                     /locus_tag="IU325_04195"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HAP5808241.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(30588..31229)
                     /locus_tag="IU325_04200"
     CDS             complement(30588..31229)
                     /locus_tag="IU325_04200"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP5808242.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            31547..34864
                     /locus_tag="IU325_04205"
     CDS             31547..34864
                     /locus_tag="IU325_04205"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAP5808243.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTIYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWNCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHDFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAVSVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(34903..35760)
                     /gene="alkA"
                     /locus_tag="IU325_04210"
     CDS             complement(34903..35760)
                     /gene="alkA"
                     /locus_tag="IU325_04210"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HAP5808244.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVAEDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            35894..37246
                     /gene="yegD"
                     /locus_tag="IU325_04215"
     CDS             35894..37246
                     /gene="yegD"
                     /locus_tag="IU325_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAP5808245.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(37258..39198)
                     /gene="yegI"
                     /locus_tag="IU325_04220"
     CDS             complement(37258..39198)
                     /gene="yegI"
                     /locus_tag="IU325_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HAP5808246.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTANHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(39195..39956)
                     /locus_tag="IU325_04225"
     CDS             complement(39195..39956)
                     /locus_tag="IU325_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HAP5808247.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVCQRLFAEAEA
                     KELAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(39953..40612)
                     /locus_tag="IU325_04230"
     CDS             complement(39953..40612)
                     /locus_tag="IU325_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP5808248.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(40833..40892)
                     /gene="ibsA"
                     /locus_tag="IU325_04235"
     CDS             complement(40833..40892)
                     /gene="ibsA"
                     /locus_tag="IU325_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HAP5808249.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(41165..41221)
                     /locus_tag="IU325_04240"
     CDS             complement(41165..41221)
                     /locus_tag="IU325_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAP5808250.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(41493..41549)
                     /locus_tag="IU325_04245"
     CDS             complement(41493..41549)
                     /locus_tag="IU325_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAP5808251.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            41828..43075
                     /gene="mdtA"
                     /locus_tag="IU325_04250"
     CDS             41828..43075
                     /gene="mdtA"
                     /locus_tag="IU325_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HAP5808252.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            43075..46197
                     /gene="mdtB"
                     /locus_tag="IU325_04255"
     CDS             43075..46197
                     /gene="mdtB"
                     /locus_tag="IU325_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HAP5808253.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVNDTQFELMMAIALVVMIIYLFLRNIPATIIPGFAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPELMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            46198..49275
                     /gene="mdtC"
                     /locus_tag="IU325_04260"
     CDS             46198..49275
                     /gene="mdtC"
                     /locus_tag="IU325_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HAP5808254.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVVVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            49276..50691
                     /locus_tag="IU325_04265"
     CDS             49276..50691
                     /locus_tag="IU325_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HAP5808255.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLTIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVHVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            50688..52091
                     /gene="baeS"
                     /locus_tag="IU325_04270"
     CDS             50688..52091
                     /gene="baeS"
                     /locus_tag="IU325_04270"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HAP5808256.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLQISAGQRDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            52088..52810
                     /gene="baeR"
                     /locus_tag="IU325_04275"
     CDS             52088..52810
                     /gene="baeR"
                     /locus_tag="IU325_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HAP5808257.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREHLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            53001..53333
                     /locus_tag="IU325_04280"
     CDS             53001..53333
                     /locus_tag="IU325_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1508 domain-containing protein"
                     /protein_id="HAP5808258.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
BASE COUNT        12334 a        14369 c        13754 g        12918 t
ORIGIN      
        1 ccatcaatcc acgctaccgc ccctggctta acagctacca gtgcactgaa ctcattgata
       61 ttgcgtactg ttactgagag acattgctgc atctccctcg aaactcaaac gctaattgtc
      121 ttaccaatcc gttctggaaa caaggaaaat ccaggaaaac tttgacgaaa agcctattgc
      181 taactatttg ttattctgat tgtttataca aaataacggc aggaatattc gcaacaaatt
      241 actttcgcca cgaatcttca ctgctgttat agtttccttg ccaaccgtca cattcggtca
      301 gattttcatt attcgcttaa cagtttctca atgcctttgc ggaacttcgc cccttctttg
      361 aggttgcgca gtccatactt cacaaacgcc tgcatataac ccattttttt accgcagtca
      421 tagctgtcac cagtcatcag catggcatca acggactgtt ttttcgccag ttcggcaatg
      481 gcatcagtca gctgaatacg cccccatgca ccaggctgag tgcgttcaag ttccggccaa
      541 atatcggcag aaagcacata tcgaccaacg gccatgatgt ctgagtccag cgtctgcggc
      601 tgatccggct tttctatgaa ttcaacaata cggctgactt taccttcacg atccagcggc
      661 tctttagtct gaatgacgga gtattcagag aggtcacccg gcatacgttt tgccagcacc
      721 tggctgcggc ccgtttcgtt gaaacgtgca atcatggcag caaggttgta gcgcagcggg
      781 tcggcactgg cgtcgtcgat cacaacgtcc ggcaatacca cgacaaatgg gttgtcacca
      841 atggcgggtc gtgcacacaa aatggagtgg cctaaaccta aaggttcgcc ctgacgcacg
      901 ttcataatgg tcacgcccgg cggacagata gactgtactt ccgccagcag ttgacgcttc
      961 acgcgctgtt caaggagaga ttctaattca taagaggtgt cgaagtggtt ttcgactgcg
     1021 ttcttggacg cgtgagttac caggaggatt tctttgatcc ctgcagccac aatctcgtca
     1081 acaatgtact gaatcattgg cttgtcgacg atcggtagca tctctttggg aatcgcctta
     1141 gtggcaggca acatatgcat cccaagacct gctaccggta taactgcttt taaattcgtc
     1201 attcttttcc tacctctaag tggctgatag tgcgtaaatt attgccatag gttagccaaa
     1261 cgttatggct atataccaaa gcttagttcc gcataacttt gcttaaacct tagaataaca
     1321 ctacacacca tcagcatctg ggttactcgg attactcgga attccgcata ctgataattt
     1381 aatcagtacc tctttccgaa taatcgtagt ccaacctggt ccttatttct ctgactcgtc
     1441 tgcattactc agaaacaaac gttatgtcgt cttttttggc atgaacgaat tcatactgca
     1501 gtgttcgatc cagaccttgc gacagcgtat acggtgccac aaaacctgaa gaatgcactt
     1561 tcgttgcgtc aaactgtgtt gttgcgcaga attttttcac gcgcactgag ctgacagcgt
     1621 attttttgcc cgtaattttg ctcaggatat caaagcaata tccacccagc attcctagtg
     1681 ggtaaggcaa gtgcatagaa gggatctttt tgttcaggct ttgttcaact tcagcaacca
     1741 actggttcat gttcaggtct ggcttatcaa cgtagttata aacctcataa cctgcggcaa
     1801 cattcttcag tttgtacttg ataaactcaa caatgtttcc aacataagcc atggacttat
     1861 agttagtccc tgcgcccacc atcataaact tgccgccagc gatctgtttc agcaagttat
     1921 agacgttacc gcggttgcgt tcaccgaaga taacggtagg acggatgata gttaatgaac
     1981 gttctgttgg tgctttgtta taccattcac gcagcacttc ctctgcctgc cacttacttt
     2041 tgccgtagtg gttgaaagga tcgtgtggat ggttttcgtc agggttgtgt ttgttcaaac
     2101 cataaacagc aacggaactg gtaaagatga tatttttaac gccatttttt tccatggccg
     2161 ccagcacatt gcgggtaccc tgaacgttga catcataata gagagaagta gggctgacgt
     2221 catcgcggtg ttccgctgcc agtagtacaa cagtgtcaaa accggctaac gcctggtcga
     2281 gtgcctgttg atcacgaaca tcgccaatct gtgtgatttc tggataaaag tggctctgct
     2341 gtttgtccag gttcttgata ttaaagtcag caattgccgt ttcaagtagt cgggttccta
     2401 cgaatccgga agctcctatg agcaaaacgt tatcgttcat aaatcacttt agtctggttg
     2461 ttacgtaaga aacacaagat aaagatgagt accttccctg agtagtcaat gctgcccagc
     2521 cccagcttta acagttagtg tgaggattat aatcttttag aacattatat ccagtaagtt
     2581 tatgaatggt cgcaaatcta ctccctccgt tctggcaatc taaagttaat cttttccaca
     2641 ttaacaatgt ggtgattaac tctgtcgata tcgacggagc tttgcccttt ttcattcacc
     2701 gcatgaacat ttgcaagtga cagcagtgtt tcttttttcg ccataaagac gccgcgaacg
     2761 tctttgcgca tgtcgaagtt catgctcaat gcgggtccaa ctgaggattc ctgcatcacc
     2821 ttgatatttc gcataaaaag atgttgcggt ttgttgtgta actccagcga cgcacgcttc
     2881 atctcaatgt tagtcagcgc cacaaaggag acagcattcc cggcagagat ttggataccg
     2941 cgcaatttat aagcaagatg ggtgttatcc atttgaatat tattcactcg gaaattttgc
     3001 ggtattgaga gatatttgcc tttaattacc ccatagccga ttaacatccc agcgctatta
     3061 atcatttcaa tattatcaat cacgaaattg tcacaaccgt aaatagcaac tgtcgcgtta
     3121 tcaatgcctg ctttcttgct gaaatccggc gtgatattgc gggctttgat attacgaata
     3181 acaaaatgtt taccattttc aacatggatc aactgccgac aatccgatcc cgtgatattc
     3241 gccacgacaa agtttttcac tgcctggtct tccgggtagt tattatcgta ggtacttccc
     3301 gcaaggccta tgccgatacc ccagttgatt ttgccgttgg tacagttgat gcgctcgatg
     3361 acatggtcag atatcaaaat atcacggtcg ttaatcgcca cgttccattc aatggcgtcg
     3421 ccctgtaaat cactaaattt acagttggtg atgttggcac cgataatctg gttatgaaat
     3481 ccctggcgta agatggcgta attagcgtgg ctaacggtca ggttatcgat ggagaggtta
     3541 cgcattactc gtttgttttt gccgccgata taaatctgcg ttaccggacc aaagccgctc
     3601 atcgtcagcc ctttgatggt gcagtcagag ccacgcacat ccagggtgat gttatgcata
     3661 ctgccaccct cctcccctgt cacctggctg ctgtcctgta agacaaatcg ccctctgccg
     3721 ttgccgcgca ggcttccaag gatgtgtaac gttttacctg caggaatgaa gatgccggtg
     3781 ttgatattgt cgcaaaccaa tccggcaggc acgacgaccg tttgcccttc gccgaaggct
     3841 tgtttaaatg aggcgatcca gtcgtgtggg ttgtagtcgt taatgttaac gctttgtcgg
     3901 gcgggaagtg cacgggcgaa aggggtatgg aggaaggcaa gcgcggagct tgccgtcagg
     3961 aaggtgcgtc gggagagttt tttaaatggc atacgttctc ctctataaag cctgcagcaa
     4021 gctggcgagt tctcgattaa tcacctgctg gttaaaatcg tgttcgactt tttcgcgcgc
     4081 acgtttgacg accggagcca gttcgtcggt gttcagttgg ctaaacgccg ccagtcgttg
     4141 cgccagtgcg cgagcatcgt tctcaggcac cagccagccg gatttatcgg cctccaccag
     4201 ttctggtatt ccgctatgca gagttgaaac aaccggaatg ccgaccgcca tcgcttccat
     4261 tagcgccacc ggaatacctt ccatatcgcc atccgcaccc gttatcgatg gcaacaggaa
     4321 gacatccgcg tcgtcgagca tcgctttcac ttcatggctc ggtttaaagc ccggcatctc
     4381 taccacatct tccagttgat attgttcgat gagcgtgcgc aggcgtcgtt cccacgggcc
     4441 aatgccgagg atgcgatagc gaaatgccac gccctgctct ttcaactgcc ggcaggcttc
     4501 gatcgccaca tgcaggcctt ttttctcggt taagcgtgcg acggagatga tttccagcgg
     4561 cgttgcgggc gctttcaccg gacgcgggct aaagcgcgtc atgtccacgc ccatacgcga
     4621 tacggcgatt ttttccctcg gacagcccat tttttgcagc cttccggccc acaaatcgct
     4681 tatcggtaac atcaggtcgc cacggcgaaa cagttgttga tattcgggag tgtagtggtt
     4741 gagcacgtcc cgactggaga tatcaatacc gtggaagata gtggcaattt tgccgcgaat
     4801 gacacccagt tcgcgtagtt ttgctgcggt tacccccgca gggccaaaat gagcgatgaa
     4861 cacatcggcg cgaaacggtg ttgcgacctg accacaaatg gcagacaaaa tcaggttccg
     4921 cgactcggcg ccatagcgtt tgaggttgag cgcctgccag gtatttttac gatgaatgcc
     4981 gcgtaacgtc tggctggcgc ggtggcgcag tttcgccact ttgcccgtag gttcgtcctg
     5041 taaccagcgg gttctggcag caaggttgta tttcgtccat gccgcgtggg tgttttgtgt
     5101 gtcgcctttt tgcagcgcga taatctccac ctcaaatccc atatcaataa acgcagtgat
     5161 ttggttaaga acgaaggttt ccgacgacaa cggaaatttc agtaaaaaga agccgacctt
     5221 catttcacct ccccgatgcg ttcaagcaca gattgcacca tctgcattcc cgtctgacgt
     5281 tcgcgactga cagcttcgtt aagtcgcgcg ttcagcgccg gaagttggcc taaggtatcc
     5341 gcaaccatcg cttgcaggct gccgtctaat aaatgacgga tatcaattgc catctctggc
     5401 agccccagct gctgcatgat cccagcggat ttatgttcat agttgatggc aatcgccgga
     5461 gtagcaaagt tcattgagat aatagcggag tgcagacgcg taccaacggt gagttcacag
     5521 gcaccgagaa ttttgcccat ttccagatcg ttaagttcat ccatcactac gtggtaacgg
     5581 gcaggatcgc tgatatgctg gcgcaggttg agcgccacca tgcggtcgtc tttgttatag
     5641 ctgtcgatgc ccgtacaggt ggagagcgca atgacctgat agccttcgtc gagaatgcga
     5701 ttgaccaccc cggcaaaggc tttttcatac gcttgttgag tggtgccgag acgtttgtcg
     5761 aacggtgcca gttcgcgcag ggtaattgcc accgttttct gttgtgcggc aacgtccagc
     5821 cagtgttgga cggcatagct ggcggtgaag tcttctgtgt ggtgatcgac cagccacgcg
     5881 gtatcgacgc catgttccac ttttgcggta gtgatattgc tgcgtttcat caaatcaagg
     5941 ctgaccgatt cgcgcaggat cagcgcgtcg cagtgaccaa aaacgtagtt cgccagttgg
     6001 ttaaattgct catcctggaa gggaccgacg ctgtgaccaa tcataaacag cggctttttc
     6061 gccataaacg tgcaaagcgc atgttcaaac tgcggcacgc cgtagagatc gacaaaaaac
     6121 gatccgccga cctggataat ggcgtcgtaa cctgacagca ggcgcacgaa gtcggtgaat
     6181 ccctgggcga tggcgatatt acgcagcttg ccagtgtcgg tgacgcgcga gagcagtacc
     6241 tggtgttgat agcggcgacg gaggactttt ttaacgcgcc cgacaacgcc cgccgcgctg
     6301 ttgtgttgtt tcatttgaag gaacagcgga tcacccatta ccgggcggtt gagcagccag
     6361 gaagaactga ccggatagcg gctcatcacg tccacttctg cgtgtggatt gaggatgttg
     6421 atggcatcaa gtaagccgcg caagatggcg ctgtcgccac gattgccgca agtgtggttg
     6481 cccagaataa gtaatttcat tttttcctca taaatgtgat gccaggtgag gctgtgtttg
     6541 ttgtgttcgt tgccgttttt tgcctgatgc gacgctggcg cgtcttatca ggcctacaag
     6601 acccgagcac aaaaccgtag gccggataag gcgttcacgc cgcatccggc attcagtgcc
     6661 tgatgcgacg ctgacgcgtc ttatcaggcc tacaagaccc gagcacagaa ccgtaggccg
     6721 gataaggcgt ttacgccgca tccggcattc agtgcctgat gcgacgctga cgcgtcttat
     6781 caggcctaca ggtcccgagc acagaaccgt aggatggata aggcgtttta cgctgcaccc
     6841 ggcaaccgtt gtcggaaccg aaaacaacaa ctcaccccgc ccgtaaaagc attttcattt
     6901 tttcgctgcg acaaaactga cgctttactt ccaccaccag cggatggcgc gacagcacaa
     6961 tcatcaccac aaacgccagc acgcctgcgg ctatttgcac cgccagcagc atccccagcg
     7021 ccagttgccc tttcagcaca atgcccagtg cataactgac caccagcgtc ggcagcgaga
     7081 gataaaacgg cagccataaa ctcaggatgt actggcgata actggaacca agaaccggtt
     7141 taatcatcac gaagtaactg agaatggtgt tgataatctg caccagcagg aaaccgagcg
     7201 tgacgccgat tgcgcccgcc atctgcccac caataacaat cgccggaata aacagaaatg
     7261 ttttgaatac gttgaattta aagctgatat cgacccgcgc tttcgccatc agcagcgaac
     7321 caatcgggtt acccaccgaa cgcagcagac ccaccacaca cagcaattgc agcaccggaa
     7381 taatgctgtt ccacttctca ccaaagacca gcggtacaaa gttattcgac accaccatca
     7441 gccccagcag agccggaaag ttgataatcc ccactaccga cagcagcttg tagaagttaa
     7501 cgcgcagctt ttcggtgtcg tcctgaattt tggcgaatgc cggaaacaac acgcgggtga
     7561 tgattgggtt cagcttcatc ggtggcacaa cggccacgtt gtacgccagg ttgtatcccc
     7621 ctgccacgcc cgcgccgaga atacgcgcca gcaccagcgt cgaaaggttg gtattgagat
     7681 agttgatgat gctgtccgcc gtcagccagg caccaaagcg taagttcggt gcaacagacg
     7741 ccagcgagaa atgcagacca gggcgataaa ttttgcggcc aaagtagcca aacagcagcg
     7801 ttcttaccgc actattgacc agataaccga ggatcgcggt catcgccagc ggccagaaat
     7861 gggcgctaac caccgtaaaa gtgaagcccg ccagcaccgc gctggtttcg atcatgccga
     7921 ttttgttgaa ctccagctcc ttctgcatca acgcgcggaa ctgttgcccg tgggggatca
     7981 ccacaaacgc cagcgacaat gttttaatca acggtgccag gtccgggtta ttcagcacgt
     8041 cgccaatggc atcactcaac aaaaacaccg ccacgcacac cacaagcccc agcccgacgt
     8101 tcagccagta cagcgtggtg agttcaaggt ggctgatttc ttttcgctga ataatcgagt
     8161 tagcgatacc aaagtcagaa agcgtatctg ccagcgcgat aatcaccagc gacacagtca
     8221 gcaggccgaa ctggtggttg tcgataatcc gcgccagcac ggtcatctgc accagcccga
     8281 ggccgatgat gatcaccgtg gcaatcgccg accacttcgc gccgctgatg gttttttcac
     8341 gtaagctcat atcaatatgc cgctttgtta acgaagcctt tgaataccgt caggaaaacg
     8401 attttgatat cgaaccagac gctccattcg cggatgtact caaggtcgaa ctcgacgcgt
     8461 ttttccattt tctccagcgt gtcggtttcg ccgcgccagc cattaatctg cgcccagccg
     8521 gtaatgcccg gtttcacctt atggcgcagc atgtagcctt caatgagctg gcgatactgt
     8581 tcgttatgcg ccaccgcgtg cggacgtgga ccgacaatcg acatcccccc ggtcagcaca
     8641 ttgataaact gcggcagttc atccagcgag gtgcggcgca gaaagttccc cactttggtg
     8701 acgcgcggat cgttctgcgt cgcctgggtc accactttgt cgttctccat cactttcatg
     8761 gaacggaact tccacacttt gatcggcttg ccatccatgc cgtagcgagt ctggcggaaa
     8821 ataaccggcc caggtgaact gagtttcacc gccagcgcaa tgcagcacag caccggggag
     8881 atcagcagca ggataagcgt cgccagcaca atgtcttccg cacgtttgag caggcggtta
     8941 accccggaaa gcggcgtgtc gtaaagcggc accaccggaa cgccgttcat ctcttcaaga
     9001 cgtgaatgga gaatgttgaa ggtaaagaca tcggggatca gcagcaccga acaggtggtg
     9061 tccgccaatt gatggaccag ttttttcact cgcgcgccgt cgcacatttg catcgcgata
     9121 tagacgttat gaatcttgcc tgctttagca tcctcgacca gctgttgcag gttacccgcc
     9181 cagtcgttag aaacgccgcc cggtttcggg tcgtggtaaa cgcccaccac ttcaaaccct
     9241 aaccacggct gattacggaa gctctccatc aacatttgtc cggcggctaa atcccccgct
     9301 acggcgacca tacgcttgtt atagccatga ttacgcagcc agcccgcccc aatgcgaata
     9361 catgaacggc aaaccaccag tccgatgctg gtcagcccat accacgccag ccagattttc
     9421 agttgcgtgt cgaaatcatt gttgaacgcc accagtccgg cgctgaaaat cacgcttaac
     9481 gtccagtttt gtagcaacag agcaaattct gtcgctgccc gaacaccgcg ccatgagcga
     9541 taaaaatcgg tgatgccgcc cagcatctgg aacaccacca gcgtaatcag cgccaccaac
     9601 aggtgcatgt agaggaatga cagtccgctc acttcgcaaa ccagccatag cccggcaaac
     9661 atgatggtga tatctgaaaa gcgttgcacc atagagatta acgatgcatt ggttttcgct
     9721 cgctcgcgct tttttagatt tgtcatcgtt gttcctgtta ttagcccctt acccgcagtg
     9781 gggtaaggga agatccgaca ttactcgttc agcaacgtca gcagagttcg cgttcgcgct
     9841 tccatcagcg gcacatcacc gcgcgattcc acattcaacc gcaccaccgg ttcggtgttg
     9901 gaggagcgca ggttaaaacg ccagtcggca aaggtcatac tgatgccatc ggtacgatcc
     9961 accgccagcg cctcacggct aaaatgctgt tcgacgcggt taatcgcctc aacgggttgc
    10021 gccagtttgc tgttgatctc accgcttgcc ggaaacgccg ccatccggtc gcgtaccagt
    10081 tcgcccagcg tttttccttt cagacacacc agttcggcga ccagcagcca cgggatcatg
    10141 ccgctgtcgc agtaagcgaa atcacggaag taatggtggg cgctcatttc gccaccgtag
    10201 atggcgtctt ccttgcgcat ccgttcttta ataaaggcgt gtccggtttt cgacatcacc
    10261 ggagtgccgc ccgcggcggt caccacatca acggtgttcc aggagagacg tggatcgtgg
    10321 atgatcttcg cgccgggatt tttttcgagg aatgcttctg ccaacaggcc gacaatgtag
    10381 tagccctcga taaactgccc tttttcgtca aacaggaaac agcggtcaaa atcgccatca
    10441 aaagcaatgc ccatatccgc gccgtgttta atgaccgcat tgcgggtgtc gtcgcgacat
    10501 tccggcagca gcgggttagg aataccgttg gggaaattac cgtccggcgt gttgtgcact
    10561 ttgatcaatt ccacaggtgc gccgagggct ttaaagcggg cttcgatggc gtccaccacc
    10621 ggacccgctg cgccgttccc ggagttgatc accagcttga gcggcgtgag gtttttgacg
    10681 ttgatataac cgaacaggtg atcaacgtaa gcgtcacgca gattgatttg ctgatagcga
    10741 ccgcgtttgg tttcatcaac gggaggaaag tcgttggctt ctgccagacg ctggacatcg
    10801 cgcagtccgg tatcgccgct gatcgggcga gccccttcgc gcaccagctt catgccgttg
    10861 taatccatcg gattatggct ggcagtaact tcaatgccgc catccacgcc gagatggaac
    10921 gtggcgaaat agatctcttc agtgccggac atgccaatat ccagcacatc gacgcccgca
    10981 tcccgtaaac cttttgccag cgccagtttt aaggtttcgc tggtgaggcg gacatcaccg
    11041 cctaacacaa tggttttcgg tttgagaaat tcgccatagg cgcgaccaat gcgccaggca
    11101 atatcttcat tcagttcttc gcctaatttc ccgcgaatat cataggcttt aaagcaggtt
    11161 aattttttca tgtcgttacc ctttttcagg caatagttgg ccctgaccga agcggccaat
    11221 tttgtttttg tcgttattta caccgcggtt tcgcattcat tgcctgatgc gacgctgccg
    11281 cgtcttatca ggcctacaaa accgaaccgt aggtcggata aggcgttcac gccgcatccg
    11341 acaaacagcg cctgatgcga tgtttacacc cgtccgtagc gatccgcgaa gcgcaccaca
    11401 tcatcctctt cgagataaga gccggagcgc acttcaatta aatcgagcgg aattttcccc
    11461 gggttttcca ggcagtgcgt cgcccccagc ggaatataaa tggactcgtt ttcaccaagc
    11521 agtttgatat caccgtcaat agtgactttt gccgttcccg cgacaaccac ccagtgttcc
    11581 gcacggtgat ggtgcatctg taccgacaag ccttcgcccg gtttcacggt gatgcgtttc
    11641 acctggtagc ggtcgcccgc gtcgatagag tcatatttgc cccacggacg atagacttcg
    11701 cgatgtaccc gatgctcatg gcgaccatcg gctttgatct gctcgaccac ttttttcaca
    11761 tcctgcaccg cgttacggtc ggcaatcagc accgcatctt tggtctgcac taccaccaaa
    11821 tctttcaccc cgacggtggt gaccaggcca gattcggcat acacatagct gttttcagtt
    11881 ttgtgattaa tcacatcgcc atggcaaacg ttgccctcgg cggtgtgggc gctgatctcc
    11941 cataatgaag accaggaacc gacatcgctc cagcccgcat ccatcggtac cacaacggca
    12001 tctgccgtac gttccatgac cgcgtaatcc accgactctt ccgggcaggc cagaaacgct
    12061 tcttcatcca cgcgaataaa atcgagatcc ggatcgacgg cgctcatcgc tttttcgcag
    12121 gcatcgagga tatccgggcg atattttttc agttcttcga gatagcgtcc ggcgcggaac
    12181 aggaacatgc cgctgttcca gtaatattcg ccgcttgcca cataggcctg agcggtttcc
    12241 agattcggtt tttcgacaaa ctgcgccact tcaaaggcca ccatatcctg ctcacccgca
    12301 gacacttcac cgcgacgaat atagccataa ccggtttctg gtagatccgg cacgatgcca
    12361 aaggtcacca gcttgcccgc ttcggcatac ggcatggcat tacgcacggc ggcacggaac
    12421 gcgtcttcat cggcaatcac atgatccgcc gccaatacca gcattaacgg gtcgctctcc
    12481 gggctatgac gttttgccgc cagcgccgcc agcgcaatgg caggtgcagt gttacgccct
    12541 gccggttcga gaataatgtt ctcggtgagt ttgttcagtt gacgcagctg ttccgcgaca
    12601 ataaagcggt gctgctcatt gcaaatcacc accgggcttt cgcactccac gccgttcagg
    12661 cgacagatgg tggtttgcag catggtgaga tcacctttca ggcataaaaa ctgcttgggg
    12721 taaagtacgc gggaaagcgg ccataagcgg ctaccggagc cacctgccat cacaactgga
    12781 tagagtttcg actgcgccat aattatcccc gaatatcatt tataaattga cgtaacacgt
    12841 tctctttatc gagcgtgcgt tcggcatatt cacgtgccac cgtgttgtac ttgggcagca
    12901 ggagcgcctg acgaatcccc gccaccagcg cctcgaccga ttccggttcc acacaaaccg
    12961 caatgcccgg aaaggtttcg caaagctgtc ccagttctgt gtgggcctct gcggtaatca
    13021 ccgcgttacc gcctactgcc agaatattgg tcagtttcga cggtaatacg gcatctgccg
    13081 cgccgcgttt ttgcaccacc agatggcaat cgcccatctt cagcagtgcg ggtaaagcgt
    13141 catacgattg cagcgggaaa aattgcatgt tgcgcagtcc acgctgctgc gccatttttt
    13201 ccagccgcgc tttgccgccg ccctgcccga caatggcaaa aatcagcggt tcatcgcgca
    13261 ggcgatcggc agcttcaata acgttttcca gtccctgctt ttcaccaata ttgccggagt
    13321 aaagaatgat ttttttgtta tccggcaggc caagctggtt acgaagggca tcaacatcgg
    13381 cgtctgcaac atgctgaaaa cgggcgattt ccgaccagtt ggggaagaaa atgatgttat
    13441 ccgccgccac gcctttttcg atggctttat tcatcatcga acgcgaaatc gtggagacgt
    13501 tatcgacgtt atgcagtccg ctacgttcga acgccgttgc cagctgtgcc actttgccgc
    13561 ctttgccttt tccggcaagg cccagcccca gcatggcgtc cacttcgtaa tcctgaatat
    13621 gcagcacggt acgcgcacca gagagtttcg ccagcaggcg cattcccggc gtgcaaaaca
    13681 gcgttggcac cacgccgata atgcgatccg gcttccagcg acgttgcgcc atcaacggga
    13741 aaaaactgct gacggcaaaa ctgccgagat gcaacaggcg tttcagggtg ctcggctgtt
    13801 ttggcacgta cagcgggcag cgccacaccg tggcggcccc ctcttctcgt ttgtagcgcc
    13861 aggcggaata gttctcgccc acctgccact gcgggtagta aggcggtgcg gtaataaccc
    13921 gcacctcatg accttgcgcc gccagccatt ccaccatttc tccggtgtat ttgccgatgc
    13981 cggttaactc cggcgagtag ttaatgccgt agaccagtat tttcataatc cgggtactcc
    14041 ggcacgcttc tcagcgagaa aataggcgcg gctgttagcg tgaacattgt cactggcgag
    14101 cagtgcgtcc ggcgtcagcc agcggtaatc gtcatgctgc tcatccggca gtaacagttc
    14161 ttcttccgct actctgaagc gaaaaccgag caccacatag tgagtggtga aatccgtgcc
    14221 ggagaagtta tcgtcataaa agtgctgcca gacaccgtaa aactggcctg ctgttatcgg
    14281 cagacgcagc cccagttccg ccatcgtcag ccgctcaaat gcggcttcca gcgtttcgtc
    14341 tttctgcacg cgccctcccg gcacaaacca gtaaccctgc gccgggcggt tggttctttt
    14401 gccaagcaga aactcgccgc gactgttctc gacaataaag tcgagagaga caagcggggt
    14461 ggagcgcact accgtggcaa agtcttcctg acgtaaaaac atcattaccc ccgaaagcgg
    14521 tcttgattct caaggaacca ctggtaagtg ctggcaagcc ccgcttccag tgagatttcg
    14581 tgataccagc caagctgatg caggcgcgtc acatcaagca gtttgcgcgg cgtaccatcc
    14641 ggtttgctgg catcaaaaac cacccgacct ttgtaaccca ccactttggc gatggtttgc
    14701 gccagctcgc ggatagtgca gtcaacgccc gtgccgacgt taatgtgcga cagcatcggc
    14761 tgggtgttct ccagccagac ttcgtgcgcc agctccatga catgaatgct cgccgccgcc
    14821 atatcatcga cgtgcaggaa ttcacgcatc ggtgtaccgc tgccccatac caccacgtcc
    14881 ggtgcattct gtgccgtcgc ctcgtggaag cgacgcagca atgcagggat cacatgcgaa
    14941 ttactcgggt ggaagttgtc atgcgggcca tacaggttgg tcggcatgac tgagcggtaa
    15001 tcgcgtccgt actggcggtt gtatgattcg cacagtttga tcccggcgat tttggcaatg
    15061 gcgtaaggct cgttggtcgg ctccagcgtg ccctgcaata actcgctttc tgccatcggc
    15121 tgttttgcca gtttcgggta gatacaggac gatccaagaa acagcagttt gttcacgtcg
    15181 ttctgatgcg cggcgtgaat gatgttgctc tcaatcatca tgttctggta gatgaaatcc
    15241 gccggatagg tgttgttggc aacaatgccg cccactttcg ccgccgccag atagacctgg
    15301 tcaatgcgtt cgctggcaaa gaaatcatgc accgcgcggc tgtccagcag gttcagctcg
    15361 tcgcgggtgc gtaataccag ttccacatca ccgcgctgtt cgagctgccg cctgatggca
    15421 gaaccgacca tcccacgatg gccagcgata aaaatgcgtt gtttactcat gcttatgact
    15481 ccagcgcgat cgccacgtcg tagccgtgag atttcagcag agagtgtttt ttcgccgctt
    15541 cgaggtcatt agccaccatt tcagacacca tctctctgag ggtgatttcc ggtttccagc
    15601 ccagtttttc gtgcgctttg gtcgggtcgc cgagcagcgt ttcaacttca gccggacgga
    15661 agtaacgcgg atcaacggcg ataatcacat cacccggttt aacgcccggc gcgtcatgcc
    15721 cggtgacgga aaccacaatg cccttctctt caacacccgt gccttcaaag cgcagtttga
    15781 tgcccagctg tgccgctgcc atttccacga actgacgcac ggagtactga acgccggtcg
    15841 cgataacgaa atcttccggc tgttcctgtt gcagcatcat ccactgcatt tttacgtagt
    15901 ctttggcgtg gccccagtca cgcagggaat ccatattgcc gaggtacagg cacgactcca
    15961 gcccctgggc gatgttggcg attgcgcggg tgattttgcg ggtaacgaag gtttcgccgc
    16021 ggcgcgggga ttcatggttg aagagaattc cgttacaggc gtacatgccg taggattcgc
    16081 ggtagttaac ggtgatccag taggcgtaca gtttggcgac agcatacgga gagcgtgggt
    16141 agaacggcgt ggtttctttc tgcggaattt cctgcaccag accatacagt tcagaggtgg
    16201 aagcctgata gaaacgggtt ttcttttcca gaccgaggaa gcggatcgcc tcgagcaggc
    16261 gcagcgtacc catcgcatcg acgtctgcgg tatattccgg tgactcaaaa gaaaccgcaa
    16321 cgtggctcat tgcgcccagg ttgtaaactt catccggctg cacttcacgc aaaatgcgtg
    16381 tcaggttgga ggtatcactc aggtcgccat aatgcagatg gaatttcggg ttgcaggtgt
    16441 gcggatcctg ataaatgtga tccacgcgct cggtgttgaa tgacgatgca cgacgcttaa
    16501 taccatgcac ctcgtaacct ttttccagca gaaactctgc caggtaagaa ccgtcttgtc
    16561 cggttacacc ggtgatgaga gcgacttttg acatgtctta ttcctctgta ttttttgaat
    16621 ttattcagtt tcaacgcgtt cgcgtatcac cactgcggga ttcccccggc aaaccacatt
    16681 tgccggaagc gatttaaaaa cactgcttcg cgcccccacg acggtgccgt cgccgatagt
    16741 gacgcctggg gcaacaaaga catcggttgc cagccagcat ttctcgccaa tcacaatagg
    16801 cgtggcgtta atggtgaaat gttgacttgc atggtcgtgg ctcccggtgc ataaataact
    16861 tttttgcgat atcaccgaat gtgcgccaat ggttatttca ccgagggtat ataaattgac
    16921 ctcatcgccg acccacgcgt aatcacctaa ggttaatttc cacggatagg taatttttac
    16981 tgacggccga ataactacgt tttttcctat ttttgcgccg aataaacgta ataaaaatgc
    17041 ccgccagcga tacaatactt gtggcgacca ggcaaataat gttgcctgta ctgcccacca
    17101 caattgcact ttaatagcgt tgccaccccg gaaccctttc ggcaccgaga atccacttaa
    17161 atcctgcatc gttttccctt atattcagct tttgttatat aaggctttcg tctttgaggt
    17221 agtacgctgg cgtaaatgcc aggataattc tgcccagaag ccaggcacat gtaatatttg
    17281 ccgttggact tttttcgcgt cagcacacaa ctccatatta ttggtggtag ataccccacc
    17341 catagaaaat tcagacacca ggccattgag ttttttaaat gcataacctg ctttatacat
    17401 tttggctgcc agcgcgtaat cagaagaaac tttatattcc aggtcataac gccatttttt
    17461 caaaccggat accgggaaaa atatcgcctg atgactggcg ggcaggctgt gataaatata
    17521 ccaacccggt ttggcgctac gtttaatttt atgcccgtcg ccaaaatcca gcagcgcatc
    17581 gccggtgatc atcacgttat ctttttgcat ttttaactta cggacaaaat atgcggcatc
    17641 ctgatgaaaa atatcgcccg agttgagaaa caacgcgaac ttgccttgtg ccatcgcaat
    17701 gcccttgttc atggcgtcgt agataccgtt atctggctcg ctgacaaagc gtaggttata
    17761 gatactattg agattttcca gatactcacg ggtaccgtcg ttggaaccgc catcgacaac
    17821 aatccattcg aagctgatat cgtccgcctg cgccagatgc gccagcgagg catgtgtttt
    17881 gactatccct tcgaggttac gaaacgcgac agtgattatg ctaagcaaca tgttcttatt
    17941 cttacctcgt aatatttaac gcttttcgca aaataaacgg acagacgatt aaaaatgcat
    18001 attccgggct aaatatcgaa ccggtaaaaa acagcgatac cggagtaaaa agatataatt
    18061 gcacgcgaaa attgcggtta tcaccaaaag cgttgatcat catttttatc actttcccca
    18121 tgtaccacag cgataaaaac atcgcgaacc aggaaaaata aataatcagc agatacaaac
    18181 cattgtctat ggtttttccg acatccgcac cgttaaatat tccgaatgat gcgacatatt
    18241 cataaagtga gccaaatctg actacaccgt caatatgggt caaagaataa ccgaccatca
    18301 ccagcggacc gacaatacga taatacgatg acgatccttc cgtacctaaa tcgcccagac
    18361 gggtggaaat ataaggaaac gcgattacca caccaaccag gaatacagcc agagaaatca
    18421 atgctaacgg taactttttc ttaatcgcct ctttattcag atattggaat gcccactcca
    18481 gcaaataaaa caggataaag gtcataaccc ctgaaaacga tccggataat attatccctg
    18541 cgagaatcat agcatctgtt ttaggcgttt tgataccaaa ctgtttgatg ctgagccaaa
    18601 ttgagattaa tgccagagcg aaaaatgccg gttcgaaata aagtgctgtc gtgcgcttgc
    18661 cgccgaattt aatgaaattc agtacatagc tgttgctgta aatcagatat ttcgaaatcg
    18721 actccataat actgctgccg ccggtgagga taatttgcgc catctccacc gccgccagcg
    18781 ccaccaccag cccgaccacc agataaaaga aacgtaatat cttgcgatgg ttgtgtggcg
    18841 aaattgtttt aaaacgaata ctccacacca tgccaataat gatcacaata tagacaaaca
    18901 gcatggttga tgtgacgtat ttactggcat ccagcgactg accaaacaga tagttaaacg
    18961 ccgtgagtcc cgcaccaatc cccagagcaa tcatcaattt tttaacgctg atgcgctcta
    19021 aaaacagcag taacaagaca ggtaaaaaag tgacgatagt gatggggaaa ctttcaccaa
    19081 gctgagcgat tttgacgtta accagcaggt agatcagcgg cagcagcagg tagctacaga
    19141 ttctgataga agttgacata ctcctccagc atctgttgtc cactgtaggc ggcgcggctg
    19201 cgttggatga actcagccag cgtggtacca aatatcgcct gcgcgatttc cggtttgctt
    19261 aactgcacca gttgcagcac ctcttcttca ctgacggttt taccaccgga tttttgcagc
    19321 acttcccgcg ccgcatcgct atgggtggca atcaccggca cgccaatcga tagcgcctca
    19381 cacaaaatca gcgggtagtt atcgacacga gaactgaaca ccagcgcatc catctgattg
    19441 agcgcgctca tcaacttgcg cttgtcagtt tcaaagccgt gattaaccac gttgccagcg
    19501 gtgaacggcg agaacttacc aaaggtatgc agttcaattt tgtcgcccag cgccatcatc
    19561 tcacgcacca gttgctggtt agtcttgccg tcgtaacgca ggtcatgcgc caccaccgcg
    19621 attttcggcc tgccctgggt ttcgcgcacc ggaggcaagt ccgccagaat cgcttcggtt
    19681 gccatatcaa tgccattatt gataatccgg caacgccctg gcccgtacag actattgaaa
    19741 gcatcagcca catgctggct gggggaaata aactgacagc ccagcgccag catctcgcgg
    19801 aataactggc gtttgcccgc caccagttgg tgtgcgcgat caatcttcac cggcggatag
    19861 ttatttaagg tcgggcattt ctggcagcct gttttccagc cttcgcaacc gtcggtaaag
    19921 gcgcagcgtc cggtaacact ccagtggtcg tgcagcgtcc agaccagagt gatatccggt
    19981 ttatggtttt tcactttttc gcaaaagcgc accacgctct taagatttag ccagtagctg
    20041 tgcagcacat gaaaatgcag gaccaccgga cccggtgtgc gagtaatggt gcgatataac
    20101 tcattgaaat tgccaaacag atcgcgatta aacagacgaa acagggcaat gttcgccatc
    20161 gcggtcatcc gcggcgtatg tttgatgacc tgcggatagt tttgatggct gacactctct
    20221 ttgccgcctt tgccgtaacc gtacacaaaa tgtgacgcca gcccctgttg cagcgcacgc
    20281 tggtggagat ctaacgccac acctgctgcc ccgccttccg ccagtcgcac attaaattgc
    20341 aaaatattca tttaattacc ttcactcgcg ctttttctcc caccaccagc gcgttgtccg
    20401 ggacagagtc gagcaccacg ctgcccgcac ccacggtgac gttgttacca agcgtgatat
    20461 caccaagaat aatgacgttg gcaccgagtt cgacgccgtt gccaatatgt ggacatgcca
    20521 tgttatcggc accacgattg ccgatagtga cgccgtggcg aatggtgaaa tcatcccccg
    20581 ccaccacgtt tttattgatc actacggcgt aaccgtgatg gatagtaaag cggcggccaa
    20641 tggttgcggc tgcctggatt tcataaccga aaaagcattc ggtgataatg cgatacagca
    20701 ccagcagcgg ggccgcccac agattgttga ggacgttctt tttgcgccac accgaacaaa
    20761 aatgagcaac acgataggca agtaccatgc agcacgggcg taaactccag ctgttggcgc
    20821 gcagatcttc cagcatcttt agcgcccccg aataccatca gccagacgtt tgccgttacg
    20881 caccgacagc agcgtcaaca aggtgcgcca ggtcatacgt ttattgcgga tctggtagag
    20941 ggtaaacagc tgatattttt tactggcacg gtcgaatttg tctttgtgct tgcggtaaaa
    21001 gtggaagtat ccggagaatt ttttcggtga cgaggtgatc tgcatttcgc cgtgattgat
    21061 atgcaggatc tgcgtcgcct cttccacttt ccacggttcg ccatactcca ccaccatccg
    21121 caggaagatg tcgtaatcct gtgctgcttt cagttcggta tcgaacaaac actctttgaa
    21181 acgccacgcc caggtaaaaa cctgattgcc aatgatattg cgtttataga acaggcgacg
    21241 tgaatacggc gatttgggat acagcggcaa acttgctggt tgggaataga cttcgccctg
    21301 gcaaacgtaa tcgttggcgt acaaaaaggc gtgagtgacc agttgctgtt tatgggcgag
    21361 gaagacgctc aggcggttgg gtgtccattc atcatcatca tcgatcccgg tgatgtattc
    21421 cccttgcgcc agcataatcg cctggttacg caccgcgcac gccccactgt taatgtcgtt
    21481 gtggatgtaa gtgatacgcg gatcgttgag ggcggtgacg tactgttgca gttgttccca
    21541 ggaggtggag caatcatcca cgatgatcat ctcccagttg ttatagtcct ggcgcaggac
    21601 cgattttatc gcccgaatcg ccagttgttg gcggttccag gttggcatat agattgagat
    21661 cagcggattg tctttcatag gttgttctcc cggcttatga gctggtttgc ttgccggatg
    21721 cggcgtgagc gccttatccg gcctacgggg cggtacgaat gcaggcgtcg ttatttcgca
    21781 tccgacttat attcgtattc gtaatagcca taatcctgat acgcgctggc gcgacggaag
    21841 atggagttca gaatcacccc tttcaccgga ataccgtttt gctcaaagcg gctcagactg
    21901 gtttccactt ctttcaatgt gttgaccgca taacgcgcca ccattaacgt ggttccgaca
    21961 tgacgaccaa caattgccgc atcggtcact gccagaatcg gcggcgtatc aatcaacacc
    22021 aggtcgtagt ttttactcgc ccagttcacc agttcggcaa agcgttcgct catcaacagt
    22081 tcagaaggat ttggcggtac ctgaccgcgc gggatcaggt caaatttggc aatagaggtc
    22141 ggtttagcag cggtagtaat atcgccctga ccaatcagaa tttccgacag gccattaacg
    22201 ttattcgtgc ccaacagctc gtgggtataa cctttgcgca tatcgcagtc gatcaacaac
    22261 acccgtttat tggtctggct gatcaccgcc gccaggttgg cgcagacaaa ggttttaccg
    22321 attgacgggc taaccccggt catcatcaac acattgttct gcgcctgcat catcgcgaag
    22381 tgcagactgg tacgcagact gcggatggct tcaatcgcca gatcggttgg attccccacc
    22441 gccagtagct ggctctgctt atagcgttta acccctttga tggttttgac gctatcgcgc
    22501 gctttctgcc attccgacag cgggatgctg gcatagacgc taataccgtg ttcttccagc
    22561 acctgtgggc tttcgatgcc gcgattaaac aacgagcgca gcagcacacc cacgatagag
    22621 agcatcaggc cgaggataat tgcgccgagg ataatcagtc ctttcttcgg tttcagcacg
    22681 ccaggctggg tgattgccgg gtcaacaatg cgcacatcgc cgacggtgct ggcttcggtg
    22741 attttcagct cctgctcttt attcagcagc tgcatataga cctgctgacc agactcgaca
    22801 tcgcgggtca gacggacaat ctcctgctgg gttttcggca tcgccgttac gcgaccgtta
    22861 agtttggctt tttcgtcttc cagcgcctga cgtttctcca gcagagtgcg gtacgccggg
    22921 tgaactttgg tgtacagctt ggagatttcc gcctctttaa aggtcagttc gttcaactgc
    22981 gcgtcgatgt tcaccatcga atcgagcacc gctttcgctt ccagcggcag atcaactgaa
    23041 tctttatcct gacggaaggc attcagtttg ttttcggcaa catcgaggcg gctacgtact
    23101 tccggtaact gttgcgcgag gaaggcgagg cttttcgacg cttccgccga tttgcgctca
    23161 atattttgtt cctgatagtt acgggcgatg ctgttaagaa tgtcgcggat ctgttcgcga
    23221 tcttcaccgg tataagtcag gctcagtacg cctgcgtctt tgccgttctc cgttaccgtc
    23281 aggctgtttt gcagttggtt gatcatcccc agcgtggagt atttggtgat ggtaaactca
    23341 ctgcccgggc tggcgtgaat agcttcaacc atcagcgtga cgccttcttt tgtcagcatc
    23401 tggcccgctt gcccacgggc gctaaagccg ccatcgctgc tcagagtgta gtttttatcg
    23461 tccagcacat taagcgtaaa cacctgatcc gccatctctt tcggacggtt aaaggtagtc
    23521 actttcaccg tctcgttctg gcgtcccatc aggcgatccc agcccgcccc gaaaatcggg
    23581 aacgtgtttt tgctcaccgc aatatcgagg tcgagatcgt ccaccgtttt accaagcacc
    23641 aggcgcgaac gaatcaactg gatctcggcg tccgatgcag gcggtttgtt ggctaacgcc
    23701 gaaccaatgt cctgcactaa cgaattgccg ctgctttgct cgatttgtac cagtgcgtcg
    23761 gcactataaa tcggcgtggc gaagaaggtg taaaccacgg cacagagggc aaatacagcg
    23821 gtgatgccaa tcacccacca gcgcgcttca atgacggtgc cgaccaggcg accaatatcg
    23881 atttcatcac tgcccgttac cggagcggca tgttgtttta ctttttctgt cattcttata
    23941 cctgctctgc gttcaatgcc tgcgcccact ggcgggcaga ccgttcaagt aatgtgtaca
    24001 ccgctgcaaa cgtttcccgg cttttgcgat acggatcggg gatttcacat tcgttatccc
    24061 agtgaccaaa cagcatcact ttgccgcgca tctcgggtgc catctcgcat aagcgttcga
    24121 tatggcgctt ttccatggtc aaaatcaggt catagttgcg acacaggcgg cggctgattt
    24181 gccgtgcaca gtgaccttcc agagacagtt gatgttctgc ggcgacgctg atagcggtag
    24241 ggtcagcgcc cttaccgacc agcgcgccga gtccagcgga ctccactttc agctccgggt
    24301 gataacgttg cagtaagcgc tccgccgtcg gggagcggca aatattgccg acacagacaa
    24361 ctaagatgtt gttaaacatg acgattacca gttatgaatg tcgctggctg tatccgtcat
    24421 gtaacggaca ccgctaatag ttggcagcaa ctgattgatc agacggttcc agcgggaaac
    24481 cggggcggtg gtgacataca ccacgtcata aggttgcagg cggaattctg tcgccatcac
    24541 cagcgacgtg gcatcggaca tatccagctg gtagatattg gcaatcttgc cgttacgccc
    24601 gccctcgcct ttcagcggac gaatgacaaa gatgccgctg gcgttggagg tggtcatgtc
    24661 gataccttca gcattgccca gggcttcagt cagggtcata ccgctaaagt ccattttcag
    24721 ggtgctctgt ttcttcactt cacccatcac aaacactttc agatcatcat tacgcggcac
    24781 gtagagaata tcgccggggt aaagcagtcg gttctgattg aggtcgccgt tttgcatcag
    24841 cgcctgcaaa gaaatgcgtt cttcacgacc attgtgtgtt aacaccacgt tgcgccagtc
    24901 agcggtgtcg gtcaggccac ctgcggcgtt gatggcgtcg agaatggtca gaggcacgtt
    24961 ggtgatcgcc tgttggccgg atttattcac ctgtccggag atataggcct tttgtgagcg
    25021 gaaggcggcg atattaacgt ccacctgcgg gtcagcgatg tacgtcgcta agcgcccggt
    25081 aatatcactg cggatttcag cgagcgtttt cccaactacg tgaaccttgc cgatatacgg
    25141 gtaaaacata gtgccgtcag gctgtaccca gttgccggtg tcgctggagc tgcggtactg
    25201 accggctggc gtggtgagtt ccgggtgatc ccagacggtg acattaagaa cgtcccccgg
    25261 cccgacgcga tactggtaat tcgcgatctc gctttccagc gtcatattgg ggcgcgctac
    25321 attcgggcgt gggcgtaatt ggtcaatcag gcgcggggtc agcggataaa cattcaccat
    25381 tttgtcgaga tcgaaatcag cgtcctgctg tttgatgacg tctttgccca tcgtcgacat
    25441 attgctgccc ggaagtactg tgcaaccgct tatcaaggtt actgacacca ataatggcat
    25501 caatttcatt ttggatttca tcattgttta tttatcactt tggcagagta attatcctgt
    25561 gcactattaa tagcaatgtc gccatgcaca tttaccttgc agttaattga ataaaaattt
    25621 aactggcatc agtcctaaaa aaattgattt catccgcagg ctattgacag aataattcag
    25681 actggtcttt caggcatcca gacacgctac cgcccctggc tttttagcta ccaatacact
    25741 gatttagctt aatttttcaa accctctctg catacaatcg ttgatgagaa agggttattg
    25801 cggaaattaa cttccgaata taaggtgaca ttatggtaat tgaatattgg ctctccaata
    25861 atgcaggagg aagtgttaca gctaacggaa tagcaggcaa ggtaacaatt cggcaattgg
    25921 ctatttttaa gaattatatt aagtgtcatt caatatggtt tttaggagtt tctttaggtt
    25981 gacaatattt aatattgcgt ctccacatgc gatatttctt aaataatgtt ttattattac
    26041 cccttttaat tcagggataa tgtgaggtta ttaccctcaa ataatatgag ataaatagtg
    26101 ctgcaacatt gcatttttgc cccgatttat ccatgatcga attgtgacat ttgtcataca
    26161 acgaataggt tttgtactta ctatggaatg gattgccgat ccgtctatct gggccgggtt
    26221 aatcacgctg attgtgatcg aactggtcct cggcattgat aacctggtct ttattgccat
    26281 cctcgccgaa aaactaccgc cgaagcagcg cgaccgcgca cgggttaccg ggctgctgct
    26341 ggcgatgtta atgcgcctgt tactgctggc gtcaatctcc tggctggtca ccctgactca
    26401 accgctgttc agcttccgct cgtttacctt tagcgcccgc gacttaatca tgctgtttgg
    26461 tggtttcttc ctgttgttca aagccacgat ggagctgaac gaacggctgg aagggaaaga
    26521 cagcaataat cccacacaac gcaaaggtgc gaagttctgg ggcgtggtga cgcaaattgt
    26581 ggtgctggac gccattttct cactcgactc agtgattacc gccgtcggga tggtcgacca
    26641 tttactgatc atgatggccg ccgtggttat cgctatcagc ctgatgttga tggccagcaa
    26701 gccgttaacc cagttcgtta atagccaccc gaccatcgtt attctctgct taagcttcct
    26761 gttgatgatt ggctttagcc tggtggcaga aggtttcggc ttcgtcattc cgaaaggcta
    26821 cctgtacgct gccattggtt tctcggtgat gatcgaggcg ctcaatcagt tggctatctt
    26881 taaccggcga cgttttcttt ccgctaacca gacgctacgc cagcggacta ccgaagcggt
    26941 aatgcgcctg cttagcgggc aaaaagaaga tgcggaactg gatgccgaaa ccgcgtctat
    27001 gctggtggat catggtaacc agcagatctt taatccgcag gaacggcgga tgattgagcg
    27061 ggtacttaat cttaaccagc gtaccgtcag cagcattatg acgtcgcgcc acgatattga
    27121 gcatatcgat ctcaacgcgc cggaagacga gatccgccag ttgctggagc gaaatcagca
    27181 tacgcggctg gtggttaccg acggtgatga cgcagaagat ttgctcggtg ttgttcacgt
    27241 tatcgacctg ctacaacagt ctctgcgcgg cgaaccgctc aacctgcggg tgttgattcg
    27301 ccagccactg gtgttcccgg aaaccttgcc gttgttacct gccctggagc agttccgtaa
    27361 tgcccgcacg cactttgctt ttgtagtgga tgagtttggc tcggtggaag ggattgtgac
    27421 attaagtgac gtcactgaaa ccattgccgg taacttaccg aacgaagtgg aagagatcga
    27481 cgcccgccat gatattcaga aaaatgcgga cggttcctgg acggcgaatg gtcatatgcc
    27541 gctggaggat ctggtgcaat atgtgccgct gccgctggat gaaaaacgtg aatatcacac
    27601 cattgccggg ctgttgatgg agtatttgca acgtattcca aaacctggcg aagaagttca
    27661 ggtgggggat tatttgctta aaacgttgca ggtggaaagc catcgcgtgc agaaggtgca
    27721 gattattcca ctgcgtaagg atggcgagat ggagtacgag gtgtgatggt atcggtcatt
    27781 ttacccgata gccgaaagga ataagatgag ggaaccgcgc cagcggcgat ttaccgccgg
    27841 gagcccgggt tgccagggtg gtggcgattg agccaccctg gcacgttcac cggcttgagc
    27901 gttccatagt agtgaggaac atcaagtgaa cggaacagcc ttcgatctta catattcaga
    27961 atataatggt gccgccgcga acgcctgatg cgacgcttta cgcgtcttat caggcctaca
    28021 ggacaggtca cctggtaacc tacatcttct ccagcaactt cttcacatct ttgccattgc
    28081 gggaatcttt attccgctcg gcccagtcat tcaggcgacg tttcgcttcg tcctgtagat
    28141 gtttgcgcag taactgatcc acttgcagac tgtaattgag ttgctgccag ttgccataaa
    28201 cccgcagcgg tactggcgtt tctttgagga aatcaatcag tttgctttcc ccgttccagc
    28261 cacccacgac ccgaatatca aactgggtgt cgcaggtttg atctgccaga ttcaacatgc
    28321 cttcccctgt cagcgccagc acaggcgatt gaccttgcat gtcgtttaac gtcacgacgc
    28381 catccttcaa cgtcaaatcg gtggtaaagc ggtcaagacg cgttacgtta tcgaagtttt
    28441 cagcggcctt cacatcacca ccattacgtt ctaccgcttg ctgaatcatc tgctggaagt
    28501 tcatcccttc catgcgcgtg tcggtcattt cgacatgtgc ctgtccttgc cagttgtggc
    28561 ggaatgcgtc ggcatctatg tcagcaccgg agaaatcacc agccagtgac atttttccgg
    28621 tcaacgaaat cggatagtta aacgccttca gaatggtacc aatctcaacg ttttccagcc
    28681 gtggctggaa gtttatccgc ggatttattg atgtcgcgtc cagcgtgccc ggcagtgaaa
    28741 cctgtccacc gttaagcttg ccctgcagtt gagtaatttc cagcaaaccc gacttgttgg
    28801 tcatttgcgt ggcaacatct gtaaaattca ttccgcgcca gcgcacgtta ctggcctgca
    28861 acaaaatatc agccgtaaag ccttgcagtc cctgatatgc cggttcatca atacgcgaag
    28921 aaatgaccgg gcgcggcaat gttgattggc tctgcccctg ctgcgcggca ccgttttcac
    28981 cattcgctgt ttcattaagc gggatgaggt tgtcaagatt cagttgcgga aattgcagtc
    29041 tcagctgcca ttcaggtttc tctgtcagcg tgacctgtgc ttgcccgctc agcgtgctgt
    29101 cattggcggt caagctgatt tggttaaatg aaaggcgttt atgtgactcc tgccactggg
    29161 cctggaaact cccctgcccc tgaatacctt gttttggtaa atctgcaccc tgcaactgcc
    29221 agttaatttg ttcaatagcc gccgttaaat catgcggata atcagaagca tctaccgtac
    29281 cgttaaggga tatcgtgaga tcgcgctgat cgcgattaac ccgcccggag aactcaaatg
    29341 aaccacgatg ttggggatct tgttccattt gcaggcgaat attgcggatt gtcacctgct
    29401 cgtcatcttc atgctggaac accagcacgc tgtccgccac cttaagactg gatatatcaa
    29461 acgaccatcc gcgatcgtct gacagatccg gcaaggtatt gtcgcgcggt gcaaccggag
    29521 cgtcttcact gcgcaccgct tccgtctgcg gcgtcagttg gatcactgcc ccttttagca
    29581 tcacctgctt aacgctcagt tgatgactca gtagtggtaa aagcgccacg tccagacgca
    29641 tgttgtcggc gcgaaccagt ggctggcttg caccctgggc ggtgagagac attcgcccgg
    29701 agaggatact aagctgcggc cagacgtgcc aacgcagtgg cccgtcgagc tgcaattgat
    29761 aaccgctacg tgcagcaact tgcttgacca tatagtcgcg gaaatcattc ggattcacca
    29821 gcaacactaa cgcagataac ccggccacca gcacgaccag gagtatcatc agcgtcgtca
    29881 gaaatcgtct catggtatcc tcaatgggcc tgaattagtc tttatcgatt cggctggcta
    29941 ccgcgccctg ctggttgcga tatttcgcat cttcacggcg gttgtaaggt cgcgccgccg
    30001 ggccggaaag cggctcaaag ctcagcgcac caattaacat gcccggacgc agcgccagcg
    30061 gcagcttacc ggagttgtag aactccagca caatgcaacc agaccagccc ggatcgatac
    30121 ggtgcgcggt gacgtgcacc atcagcccca gacgcgccaa agaggaacgc ccgtccagcc
    30181 agcccaccag atcggctggc agcgtcaccg actccagcgt caccgccagc gccagctctc
    30241 ctgggtgaag atagaacgcc tcgccctcgt cgagaacgat ctcatcgctc atcacgcggt
    30301 caagcgcggc gctcacttca tctttgggac cgctcagatc gataaacgct gccgtgtgac
    30361 cacggaaggt acgaaattta ttgcccaggc gtacatccac cgtcgcgccg ttaatacgct
    30421 ccactggcgg acgtgggttg atcgacaaac ggccttcatc aagccaggct tcaatatctc
    30481 ggtcacacag acgcatggca ttttctcctt tcgcgcatca ctcccttaac gccaattacg
    30541 tcaagggcat accaggttat cactgaacgg tacacaattt atcaagctta ttcaaagaac
    30601 tgacttattt tcgctttcaa tatatcgatc gcgatgcggt ttttcccgcc gcgcggcacg
    30661 ataatgtccg catattgttt agaaggctca atgaattgca ggaacatcgg gcgcacggtt
    30721 ttttgatatt gcgccatcac ggaatccatt gaacgcccac gctcgttaac gtcacgcttg
    30781 atgcggcgca tcaggcagat atccagcggg gtatcaacga aaatggagaa gttaagttcg
    30841 tcacgcaaac gcgcatccgt cagcaacaaa atgccttcga gaatgatgac cttcttcggc
    30901 tcaaccgtca ccgtttcttt catacgcgta tgttcaacat agctgtaaac cggcaggtca
    30961 attgccgagc cgcgtttcaa cgcttgtaaa tgctcaagca acaggctgtg atccatcgcg
    31021 ctgggatggt cgtagttggt cttaacgcgt tcttccatcg acagatggct ttgatctttg
    31081 taatagcagt cttcgggaat tacgccgatg tgttcatcac cgacttgctc acgtaattca
    31141 cgataaaggg tactggcaat aagactcttg ccggaagccg atgcgccagc gataccgata
    31201 atgacgcact gatgagactg atcagtcata tatttagcga cctgattaac ctggatgtta
    31261 ggaagggggc gacgaagcgc caaacgcggc aattataggg atttcatccg tctgatacca
    31321 gtcgaatagc gttgccgcgc gctcagagtt aattgttgac aaagaattcc cggtggcaaa
    31381 ttacgttgat cagttttatg taaggtaaaa aatgttatac gcagttgcgc aaattatccg
    31441 cctttacgtc actttatgag caattcgcat ataaaatgta aaacttttgt actagcataa
    31501 acacagaaac gaatactggc gacctggtct tgcggataaa gcggtaatga gcaaacaatc
    31561 acagcatgta ttaattgccc tgccccaccc gctgcttcac ctggtcagtt taggtttagt
    31621 ctcgtttatc tttacccttt tctcgcttga gctttcgcag tttggcaccc aactcgcccc
    31681 actgtggttc ccgacgtcca tcatgatggt ggcgttttat cgccatgccg ggcgcatgtg
    31741 gccgggaatt gcgctgagct gctcgctggg aaatatcgcc gcatccatcc tgcttttttc
    31801 caccagctcg ctgaacatga cctggacgac catcaatatt gttgaagccg tggtcggggc
    31861 agtgctgcta cgtaaattgc tgccgtggta taaccccttg caaaatctgg ctgactggct
    31921 gcgtcttgca ttcggcagcg ccattgttcc gcctctgcta gggggtgttc tggttgtcct
    31981 gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt cagaatccat
    32041 cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc tgctgcgcca
    32101 tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac tgacgttaag
    32161 ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc tgttgatgtg
    32221 gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca cggtgatgat
    32281 ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta catacctgat
    32341 gagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca tcatgacgat
    32401 ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa cccgttttcg
    32461 gaacgccatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg gacaatggct
    32521 gcaaaccaac aaagcgctct gccagtttct cgggtacagt caggaagagc tgcgcggact
    32581 cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac aggttgaaaa
    32641 gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca accgcaatgg
    32701 cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg gcacgccgct
    32761 ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac aggtgaatca
    32821 gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca tctgggagtg
    32881 ggagttgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt atgaaattcc
    32941 tccacatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg aagatcgcca
    33001 gcatgccgaa aaagtgattc gtgattcgtt gcaatcacgc tcgcccttta agctggaatt
    33061 tcgcattacc gtaaaagacg gtattcgcca tatccgcgcc ctcgccaacc gggtactgaa
    33121 taaagaaggc gaagtcgaac gcctgctcgg cattaatatg gatatgaccg aggttaaaca
    33181 gcttaacgag gcattgtttc aggaaaaaga gcgcctgcac attacgcttg attccatcgg
    33241 tgaagccgtg gtctgtattg atatggcgat gaaaattacc tttatgaatc cagtggcgga
    33301 gaagatgagc ggctggacgc aggaagaagc gttaggtgtt ccgctcctga cggtgttgca
    33361 tattactttt ggcgacaacg gaccattaat ggagaacatt tacagtgccg acacctcacg
    33421 ttccgcgatc gaacaagatg tggtgttgca ctgtcggagc ggcggcagtt acgacgtgca
    33481 ttacagtatt acgccgttaa gtactctgga cggcagcaat attggttcgg ttctggtgat
    33541 tcaggacgtt accgaatcac gcaaaatgct gcgccagctg agctacagcg cctcccatga
    33601 tgcactgacg catctcgcca atcgcgccag ttttgagaaa caactgcgta tcctgctgca
    33661 aacggtaaac agtacacatc agcgacatgc cctggtgttt atcgatcttg atcgctttaa
    33721 agcggtgaat gacagcgccg ggcatgcggc gggtgacgct ttgctgcgcg aactggcgtc
    33781 gttaatgctg agtatgctgc gttccagcga cgtgctggcg cgactcggtg gggatgaatt
    33841 tggtctgctg ctaccagact gtaatgttga aagcgcgcgt tttatcgcta cacgcattat
    33901 cagtgccgtg aatgactatc actttatctg ggaaggacgt gtacatcggg taggtgccag
    33961 tgccgggatt accttgattg atgacaacaa tcatcaggcg gctgaagtga tgtcgcaggc
    34021 tgatatcgcc tgttatgcct ccaaaaatgg tggccggggc cgggtgacga tttacgaacc
    34081 gcagcaagct gccgcacata gcgagcgggc agcgatgtcg cttgatgaac agtggcggat
    34141 gattaaagag aatcagttga tgatgatcgc ccacggtgtc gcttcgccac ggatcccgga
    34201 agcacgtaat ttgtggctga tttcacttaa gctctggaat tgcgaaggcg agattattga
    34261 tgaacaaaca tttcgtcgta gcttcagcga tccggcactt agccatgctc ttgaccgacg
    34321 ggtattccac gattttttcc agcaggccgc caaagcggtt gccagtaaag gcataagcat
    34381 cgccctccct ctttccgttg ccggtttgag tagcgccacg ctggtgaatg atctgcttga
    34441 gcagctggaa aatagccctc taccaccacg gttattacat ctgattattc cggctgaagc
    34501 gattttagat cacgcagtaa gcgtgcaaaa actgcggctg gcgggatgtc ggatagtgct
    34561 cagccaggtg ggccgcgatc tgcaaatctt caactcgctg aaagcgaata tggcagatta
    34621 cctgctactt gatggtgagt tatgcgccaa cgtgcagggt aatttgatgg atgagatgct
    34681 gattacgatt attcaggggc acgctcagcg actcgggatg aaaaccatcg ccgggccagt
    34741 cgttttaccc ttagtgatgg atacgctttc tggcatcggc gtcgatctga tttatggtga
    34801 tgtgattgcc gatgcccaac cgctggattt gctggtgaat agcagttatt tcgcgattaa
    34861 ctgagggagc gtttgcccat tgcctgatgc gacgtatcag gcctacaatt cgtccgttcc
    34921 gtctggttgc cagccttccg tataccagat atgcaacagc gcataagaac gccagggctt
    34981 ccagcgctcg gcataacggc ggatttgtgc cggtgtcatc cccggaaatc gctgtttaat
    35041 cagataatca tccggcagaa aaacatcttt cgcctgccag ccacgcaaag cgaaataatt
    35101 cgccgtccag cgcccgatac ccggaaaagt ttgcagcgtt ttcatcgcct gctccacatc
    35161 gcccggaatt gtcattggta agctgccctc cagcgccgca tttgccagat gaatcagtgc
    35221 ctctgcccgt ttcaacggca tgcctaacgc ttttaatgcc tgcgggtcgg ctgctgccag
    35281 ccgctgaggc gtggggaagc agatatactc cggaaaatca tccagccgtt cgccataaag
    35341 ctgtacaact ttggcagtca attttgccgc catcgccacg ctcaccagtt ggcctaaaat
    35401 cgcccgcaca ccctgctcaa aagcatcaat acagccgggt aaacgcaatc cgggccgcgc
    35461 cgcgcctaac ttgcccaacg caccgttaac aatctgcggg ttacattgca gatcaaacag
    35521 gcggctcatt ttcgccagac actcagcggc aacgggttct aaacctgcac ttaaatttat
    35581 gtgcagagta tggcgggcta tatccggaat agcagtcacc acgccgcgat attcgcccac
    35641 cgccagacta cgggcataat aatcctcagc gaccgtttcc acaccactca ccgcacgggc
    35701 ggcgagaaat cccaacatcc acgaccagtc atacggcggc tgccagttca gggtatacat
    35761 cgcatctcct tattcatccg ctttcagcat aaacgttatt cagacgctac gctttgcttt
    35821 catattccgg ttgtcgcgac ggcaacattt cgctaaagtc acgccccttc ttcaccggca
    35881 tggggattaa ttcgtgttta ttggttttga ttacggtaca gcaaactgtt cagtggcggt
    35941 catgcgtgac ggtaaaccgc aattactgaa aatggaaaac gacagcacgc tgctgccttc
    36001 aatgctttgc gcgccaacgc gtgaagcagt aagtgaatgg ctgtaccgcc atcatgatgt
    36061 tccggcagac gacgatgaaa cgcaggcgct gttacgtcgg gcgattcgtt ataaccgcga
    36121 agaagatatc gatgttacgg cgaaaagcgt gcagttcggt ctttcctcac tggcacagta
    36181 cattgatgat ccagaagaag tgtggtttgt gaaatcacca aaatcgttcc tcggtgccag
    36241 cggcttaaaa ccgcagcagg tagcgctgtt tgaggatctg gtctgcgcaa tgatgttgca
    36301 cattcgccag caggcgcagg cgcagctgcc agaagcgatt actcaggcgg tgattggtcg
    36361 cccgatcaac ttccaggggc tgggcggcga tgaagcaaac gcccaggcgc aagggattct
    36421 ggaacgcgcg gcgaagcgtg ccggattcag ggacgtggta ttccagtacg agccggtcgc
    36481 ggctgggctg gattacgaag ccaccttgca ggaagaaaaa cgggtgctgg tggtggatat
    36541 cggcggtggt acgactgact gttcattgct gctgatgggg ccgcagtggc gttcgcgtct
    36601 cgatcgtgaa gccagcctgc tgggtcacag tggttgccgt attggcggta acgatctgga
    36661 catcgcactg gcgtttaaaa acctgatgcc gttactgggc atgggtggcg aaaccgaaaa
    36721 aggcatcgcc ctgccgattc tgccgtggtg gaatgcggtt gccatcaacg acgtacctgc
    36781 gcagagtgat ttctacagta gtgccaacgg tcgtctgctt aacgatctgg tacgcgatgc
    36841 ccgcgaaccg gaaaaagtgg ccctgctaca gaaagtctgg cgtcagcgtt taagctatcg
    36901 cctggtgcgt agcgcagaag agtgcaaaat tgctctttca agcgtagcgg aaacccgcgc
    36961 ctcactgccg tttatcagcg atgaactggc tacgctgatt agccagcaag ggctggaaag
    37021 cgccctcagc cagccactgg cgcggattct ggaacaggtg caactggccc tggataacgc
    37081 ccaggaaaaa ccggacgtta tctacctgac cggcggtagc gcccgttcgc cgctgattaa
    37141 aaaagcgctg gcagaacagt tgccgggaat tccgattgca ggcggcgatg actttggctc
    37201 cgtcaccgcc gggctggcac gctgggcgga agtggtgttt cgttaaaaga aaatggctca
    37261 gcagcggctt aaatctgcct gcgcctgagc cagtttttct gccgcctgac gtaacggttc
    37321 catcaacggc atggtccgtg ctggagcatg aaggcggaat cgctgcaact ctgtcgcgcc
    37381 gacagtcaac gtcgattcca gccgatgtcg tttggcggtc attttcgcca ttaccgcttg
    37441 tctgtctgct ggcgttatcg cttcattcgg cctgaacaca aaacggcgtt cacagctcgc
    37501 tttccagtcg ataaccgcct gggtcagatg gtcgccaaac cctttcactt gcttaacgcc
    37561 acgacgggta acatctgcgg ctgtttcaat accaaaagac cgtaacgccg ctttacgcgc
    37621 agggccaacg ccgggaatag aggcaacatc aataaaaaat ccctccagaa acttctgctt
    37681 ctgccgttcc cttgcagtgt catgaagtgc tgccagagcg cgtttctctt cttccggtaa
    37741 cccgagaatt tcgtccttca ttttttcgag catcgtccgt ttggcgataa aaccttccag
    37801 gccgcctacc tgctggatct ggcggactaa atggtcatag tccattttcg cacgattaaa
    37861 tgcctcacgg cgttgctgaa cttctgcttt gtacgctttg cttgtcagac tgccaataat
    37921 ccagatagcc gccagcacag gaaccaaaat aatataacgc ggttctgcct gaaggccgca
    37981 aagcagcagc gataatgctg acagtgcgat ctcaagcaga atgatgtatt cgcgccgtaa
    38041 caggcctaaa ggaagcggcc tgccagtcgg ttggaaatga tcgggtaatg gcagttgcaa
    38101 tgctggcggt gctactgacg ccatcaccat cgcccagact tttgccagca caaaatcccc
    38161 gccggtggta atgacctctt cgccgagatc aataaaatag ataacgcctt gattatccag
    38221 cgcacaccac gggcagtcgg tcaaatgagc gggataaaca tgcattgccg aaacgggaca
    38281 tttctttaac tgttggcgca gagaatcaag tgccgctacc cacgctttag ccgtcggacg
    38341 cccggttgcc acgccacttt ccgtgaatgc ctgctgaaac atggcttcaa catcactcgg
    38401 taacatcgat agcggaatcg atcgtggcgg cggttttaaa ccacgtcgct gattatctga
    38461 cgcgtaggca taacggaaat gggtaatatc cgtctccagc gcattacccg catcagagat
    38521 aagcggcaca ccagaataag gatgccgacc accaaacaag acgtgaaata tcagcaaggc
    38581 aaggccaaaa ttatcgtgat tcgcggtgcg ttcaaagccg acaaatgacg acaaggtttg
    38641 tagctctggc ggcgtaaaat gcgacacacc gacttcgcat aaatgcaacg tgccattagc
    38701 gttaatctga aaggagtcgc tatcaatcaa taccactttg ctgtcgcgac ctaccataaa
    38761 actgttctgg tttacgtcac ccacgacgtg cccgtgctcg tgaaccgtag caaaagatga
    38821 agcaatattg cgcgcaacat agagtagaaa atcccacgca caatgagggt aacgttgacg
    38881 acgatgtgcc gggctataga tcatatgaat cggttcttta ccagaaactt ttggcatcat
    38941 aaaaccgata acttttccgc ctcgtccacc gtgaagcgtt gcctgcggcc aggcgacata
    39001 attcaacaat tgcgcgtcag ctgtcgcagc cataaaggca agtttgtcct gttttaaggc
    39061 gggtggcggc gtgtgataaa tcttggcgac gctatcgaca aactcattga tatcataaac
    39121 cgcgccttca ccgcctttgc ccagttcacg gcctggcgta acgcactcac cagtagcagt
    39181 atataaagtg ggtttcatgg taaccacagc gccagtgcca acgttttatc atcatccgta
    39241 cgctcgttta ccgccgggct ggacaaaaac tgctttaaca attcaggtaa taaatcgagt
    39301 tgttcctgcg ttgctgacgc cagtccattg aaaaacgggg taaagaaagg tacatgagga
    39361 gaattatcca gcatatttaa cgccagtcgc tggataccat ccgtaaatgc tgcaacttta
    39421 tgcacacgct cagtgctggt gaaagtttcc agtctggaaa cggcatcttc atcggtaatg
    39481 aagtgcgtca tattggcata ttcacccacc atcggcgtaa gcggcaattg cagaccgtga
    39541 ccaaaatcca ccaccacgcc gccatcgcca atctgcataa tcaaggtgcc gtcaggcgat
    39601 gaaatcaatc ccagaaacgt gcaggcaaaa tcgcgaactg ctaattcttt ggcttccgcc
    39661 tcggcaaaaa gtcgctgaca aacggtcagc acaatatccg tggcgaggat atcattaagc
    39721 cccaattccc caccctgcac tttttgcgac atataagcca tcgcttcatt gacggcgagc
    39781 gtcgcgcctt ctcctccctg cgaaacgcta cctgcgccat cagcaaggaa catcaccagt
    39841 aatggctgct ggtcatttag ccaggcaacc tgcatctggc aggcatcctg gcaaggcaag
    39901 tccgcgctga tgtgcgatgt tccaacagcg gatgcataaa ccagacgcca gctcacactg
    39961 acgtccagcc ttttggcgct tccagcacaa cttccgttcc cggcgtggag cgggaaaccg
    40021 aacgcagcga actggataac cagctaaaca gttcacggaa ttgtaatcct tgcagaggca
    40081 aaggctgacg aacgctgatt tgggccagcg tcttcatatc cgccccctga acaccgatgg
    40141 aaaagaaagc aaacctttta tcttcttccc cccgaaacac tttgttggct gcagcctgcc
    40201 actcatcggt tggtgcacca tcggtaatca ggaaaatcca cgggcgataa tacgaaatac
    40261 catttgcacg atactcacgt ttccgctcct ctaccatatc cagggctttg gtaatggccg
    40321 cacccattgg tgtatcgccc tgggcaaaca ggatgggcgg aaaaaaatta gcggcgctgg
    40381 taaagggttg ttccacatgc accgggccga acgtcacaat gccgagttcc actcttttta
    40441 aagccagcgg atcggcaagc agttcatcac gaaaggtcac caatccggcg ttaagttcat
    40501 taataggtct gccgctcatc gagccagaaa catccagcag caaaatgcag gggcaacgtg
    40561 gttccgggtt actggcaaaa tcgctggtgg caaacgtgat ttgttcgctc ataagaaatc
    40621 ctttcttaga gaattatcga ggtccggtat cataccgact gcaataatga aataagagca
    40681 aacgaagaaa ctttcagaat taggcccggc ggcgcagaaa attttgctat tcgcctcgca
    40741 agaacggaga gccgtcgatt gacactaagg gcggagtgac ataatttcag gagtgagggt
    40801 tagggagagg tttccccctc cccctggtgt tcttagtaag cctggaagct aatcactaag
    40861 agtatcacca gtatgatgac gtgcttcatc ataacccttt ccttattaaa agccctcttc
    40921 tccgggagag gctttcccgt ttcagcgtcc cgctgaaatc atcggcttac ctcctttcgc
    40981 cctgaatgca gtctatcgct agcgcgtgaa taaaatagtt tcctctgtga ttattgcgta
    41041 acgactcgca aagaatattt gctggcagga tcgcagacta caaagcctgc ggattgacaa
    41101 tcttatcgtg aaggcatact ttcaggagtg agggtagagc ggggtttccc ccgccctggt
    41161 agtcttagta agcggggaag cttatgacta agagcaccac gatgatgagt agcttcatca
    41221 tgaccctttc cttatttaag gccccttcct cgggaggggc tttcccgttt cagcgtcccg
    41281 ctgaaatcgt cggcttatct cctttcgcca tgcacttagt ctatcgctga cttgcagata
    41341 aaatagtttc cagtgtgatt attgcgtaac gtctcgcaaa gaatttttgc tggcaggatc
    41401 gcagacggca aagcctgcgg attgacaatc ttatcgtgaa ggcatacttt caggagtgag
    41461 ggtagagcgg ggtttccccc gccctggtag tcttagtatg ctgggtagct aatcactaag
    41521 agcaccacga tgatgagtag cttcatcatg accctttcct tatttaaggc cccttcctcg
    41581 ggaggggctt tcccgtttca gcatccggct gaaatctgcg gcttacctcc tttcgccctg
    41641 tatgtagtct atcgctaacg cgtagataaa atagtttcct gtgttattac tggatgcgtg
    41701 ctcgcaaatg tgcccgccat tcagacgatt cccgatagtg tttcataatt cctccatttt
    41761 tctcccttat tggctggcta cactagtatc attccgcgaa acgtttcagg aagagaaact
    41821 cttaacgatg aaaggcagtt ataaatcccg ttgggtaatc gtaatcgtgg tggttatcgc
    41881 cgccatcgcc gcattctggt tctggcaagg ccgcaatgac tcccagagtg cagccccagg
    41941 ggcgacgaaa caagcgcagc aatcgccagc gggtggtcgc cgtggtatgc gttccggccc
    42001 attagccccg gttcaggcgg cgaccgccgt agaacaggca gttccgcgtt acctcaccgg
    42061 gcttggcacc attaccgccg ctaatactgt tacggtgcgc agccgcgtgg acggtcaact
    42121 gatggcgtta catttccagg aaggccagca ggtcaaagca ggcgatttac tggcagaaat
    42181 tgaccccagc cagttcaaag ttgcattagc acaagcccag ggccaactgg caaaagataa
    42241 agccacgctt gctaacgccc gccgtgatct ggcgcgttat cagcaactgg caaaaaccaa
    42301 tctcgtctcc cgtcaggagc tggatgccca acaggcgctg gtcagtgaaa ccgaaggcac
    42361 cattaaggct gatgaagcaa gcgtcgccag cgcgcagctg caactcgact ggagccgcat
    42421 caccgcacca gtcgatggtc gcgttggtct caagcaggtt gatgttggta accaaatctc
    42481 cagtggtgat accaccggaa ttgtggtgat cacccagacg catcctatcg atttagtctt
    42541 taccctgccg gaaagcgata tcgctactgt agtgcaggcg caaaaagccg gaaaaccgct
    42601 ggtggtagaa gcctgggatc gcaccaattc gaagaaatta agtgaaggca cgctgttaag
    42661 cctcgataac caaatcgatg ccacaaccgg tacgattaaa gtgaaagcac gctttaataa
    42721 tcaggatgat gcgctgtttc ccaatcagtt tgttaacgcg cgcatgttag tcgacaccga
    42781 acaaaacgcc gtagtgatcc caacagccgc cctgcaaatg ggcaatgaag gccattttgt
    42841 ctgggtgctg aatagcgaaa acaaggtcag caaacatctg gtgacgccgg gcattcagga
    42901 cagtcagaaa gtggtgatcc gtgcaggtat ttctgcgggc gatcgcgtgg tgacagacgg
    42961 cattgatcgc ctgaccgaag gggcgaaagt ggaagtggtg gaagcccaga gcgccaccac
    43021 tccggaagag aaagccacca gccgcgaata cgcgaaaaaa ggagcacgct cctgatgcag
    43081 gtgttacccc cgagcagcac aggcggcccg tcgcgccttt ttattatgcg tcctgtcgcc
    43141 accacgctgc tgatggtggc gatcttactc gccgggatta tcggttatcg cgccctgccc
    43201 gtttcggcgc tgccggaagt ggactatccg accattcagg tggtcacgct ctacccaggt
    43261 gccagcccgg atgtcatgac ctctgccgtt accgcgccgc tagaacgcca gttcgggcag
    43321 atgtctggcc tgaaacagat gtcgtcgcaa agttccggcg gtgcgtcagt tatcactttg
    43381 cagttccagc taacattacc gctcgatgtc gccgagcagg aagtgcaggc cgcgattaac
    43441 gctgcgacca acttgttgcc gagcgatctg cctaacccgc cggtttacag caaagtgaac
    43501 ccggcagatc cgccgatcat gacgctcgcc gtcacctcaa ccgccatgcc gatgacgcaa
    43561 gtggaagata tggtggaaac ccgcgtcgcg cagaaaatct cgcagatttc cggtgtcgga
    43621 ctggtgacgc tttccggcgg tcagcgtccg gctgttcgcg tcaaacttaa cgctcaggcg
    43681 attgccgccc tcggcctgac cagcgaaacc gtgcgcaccg ccattaccgg cgctaacgtt
    43741 aactcggcaa aaggtagcct cgacggccct tcccgtgcgg ttacgctttc cgctaacgac
    43801 cagatgcaat ccgccgaaga gtatcgccag ctaatcatcg cctaccagaa cggcgcgccg
    43861 attcgtcttg gcgatgtcgc aaccgtagag caaggtgcag aaaacagctg gcttggcgca
    43921 tgggcgaaca aagaacaggc cattgtgatg aatgttcagc gccagcccgg tgctaacatt
    43981 atctccaccg ccgacagcat ccggcagatg ctgccacagc tcaccgagag tctgccgaaa
    44041 tcggtgaagg taacagtgct ttccgatcgc accaccaata tccgcgcatc ggtcaacgat
    44101 acccagtttg aattgatgat ggctatcgcg ctggtagtca tgattatcta cctgtttttg
    44161 cgcaatattc cggcgaccat cattcccggc ttcgcagtgc cgctgtcgtt aatcggcacc
    44221 ttcgcggtca tggtgtttct cgatttttca atcaataacc tgacgctgat ggcgttaact
    44281 atcgccaccg ggttcgtggt cgatgacgcc atcgtggtga tcgaaaatat ttcccgctat
    44341 atcgaaaaag gcgaaaaacc gttggcggcg gcgctcaagg gcgcaggtga aatcggcttt
    44401 accattatct cgctgacctt ctcgctgatt gcggtgttga tcccactgct gtttatgggc
    44461 gatatcgtcg ggcgactgtt ccgcgaattt gctattaccc tggcggtagc gattttgatc
    44521 tcagcggtgg tgtcgctgac cctgacaccg atgatgtgcg cgcggatgct cagccaggag
    44581 tcgttgcgta aacagaaccg cttctcccgt gcctcggaaa aaatgttcga caggataatc
    44641 gccgcctatg gtcgtggact ggcgaaagtg ctgaatcatc cgtggctgac cttaagcgtg
    44701 gcactcagca cgctgctgct tagcgtgctg ctgtgggtgt tcattccgaa aggtttcttc
    44761 ccggtacagg acaacggcat tattcagggc actttgcagg caccgcaatc cagctccttt
    44821 gccaatatgg cccagcggca acgccaggtc gcggacgtga ttttgcagga tccggcagtg
    44881 caaagtctga cctcatttgt tggcgttgat ggcactaacc cgtcgctgaa cagtgcgcgt
    44941 ttgcagatca acctcaaacc gctggatgaa cgtgatgatc gggtgcaaaa agtcatcgcc
    45001 cgtctgcaaa cggcggtgga taaagtgccg ggcgtcgatc tcttcctgca accaacgcag
    45061 gacctgacca tcgatactca ggtcagccgc acccagtacc agtttacctt gcaggccacc
    45121 tcactggatg cgctcagtac ctgggtgcca gagttgatgg aaaaactcca gcaactgcca
    45181 cagctttctg atgtctccag cgactggcag gacaaagggc tggtggcgta tgtcaatgtt
    45241 gatcgcgaca gcgccagccg tctggggatc agcatggcgg atgtcgataa cgccctgtac
    45301 aacgcgtttg gtcagcggct gatttccact atttatactc aggctaacca gtatcgcgtg
    45361 gtgctggaac acaacaccga aaatacccca ggcctcgcgg cgctggatac cattcgcctg
    45421 accagcagcg acggtggcgt ggtgccgcta agctcaattg ccaaaattga gcagcgtttt
    45481 gcgccgctct ccatcaacca tctggatcag ttcccggtaa cgaccatctc ctttaacgtg
    45541 ccggataact attcgctggg tgatgcggtg caggcgatta tggacaccga gaagacgctg
    45601 aatctgccgg tggatatcac cacgcaattc cagggcagca ccctcgcctt ccagtcggcg
    45661 ctgggcagca ctgtctggct gattgtcgcg gcggtggtgg cgatgtatat cgtgctcggc
    45721 attctgtacg agagctttat tcacccgatc accattctct cgacgctacc caccgcaggg
    45781 gtcggcgcac tgctggcatt aatgattgcc ggaagtgagc tggatgtgat tgcgattatc
    45841 ggcattattt tgctgatcgg tatcgtgaag aagaacgcca tcatgatgat cgacttcgcg
    45901 ctggctgctg agcgcgagca aggcatgtcg ccgcgcgatg ccatctacca ggcttgtctg
    45961 ttgcgttttc gtccgatcct gatgaccacc ctggcggctc tgcttggcgc gctgccgctg
    46021 atgttgagta ccggggtcgg cgcggaactg cgtcgtccgt taggtatcgg catggtcggc
    46081 ggtctgattg tcagccaggt gctgacgctg ttcaccacgc cggtgattta tctgctgttc
    46141 gaccgcctgg cattgtggac caaaagtcgc tttgctcgcc atgaagagga ggcgtaagtg
    46201 aagttttttg ccctcttcat ttaccgcccg gtggcgacga ttttactgtc ggtcgccatt
    46261 accctctgcg gcatactggg cttccgtatg ctgccggtcg ccccgctgcc gcaggtcgat
    46321 tttccggtga ttatggtcag cgcctcgctg cccggtgcgt cgccggagac aatggcgtct
    46381 tccgttgcca cgccgctgga gcgctcactt gggcgcattg ccggggttag tgaaatgacc
    46441 tccagtagtt cgctcggcag cacgcggatt attttgcagt ttgattttga ccgggatatc
    46501 aacggcgcag cgcgtgatgt gcaggcggcg atcaacgctg cacaaagttt gctgcccagt
    46561 gggatgccca gccgtccgac ctatcgcaaa gcgaacccgt cggatgcgcc aattatgatc
    46621 ctcacgctga catccgatac ttattctcag ggtgaactgt acgatttcgc ctcgacgcag
    46681 ctggctccga cgatttcgca aatcgacggt gttggtgatg tcgatgtcgg cggcagctca
    46741 ctgcccgccg tacgcgtcgg gctgaatccg caggcgctgt ttaatcaggg cgtctcgctg
    46801 gacgacgtac gcaccgccat cagcaatgcc aacgtgcgta aaccacaggg ggcactggaa
    46861 gatggcactc accgctggca gatccagacc aatgatgagc taaaaaccgc cgctgaatat
    46921 cagccgttga ttattcatta caacaacggc ggcgcggttc gtctgggtga tgtggcgacg
    46981 gtgactgact cggtgcagga tgtgcgcaac gccgggatga ccaacgccaa accagctatt
    47041 ttgctgatga tccgcaagct gccggaagcc aatattatcc agacggttga cagcatccgg
    47101 gcaaaattac cggagttgca ggagaccatt ccggcggcga ttgatctgca aattgcccag
    47161 gatcgctccc ccaccattcg cgcctcgctg gaagaagtcg agcaaacgct gattatctcg
    47221 gtggcgctgg tgattctggt ggtgttttta ttcctgcgct cgggtcgcgc cactattatt
    47281 cccgccgttg tggtgccggt ttcgctgatt ggtacgtttg cggcgatgta cctgtgcgga
    47341 ttcagtctca ataacctgtc gttaatggcg ctcaccatcg ctactggttt cgtggtggat
    47401 gacgccatcg tggtgctgga aaacattgcc cgtcatctgg aagcggggat gaaaccacta
    47461 caggccgcac tgcaaggtac tcgcgaagtc ggttttacgg tgctgtcgat gagtctgtca
    47521 ctggtggcgg tgttcctgcc gctgctgttg atgggcggat tgccgggccg actgttacgc
    47581 gagtttgccg tgacgctttc tgtcgccatt ggtatttcgt tgctggtttc cctgacgcta
    47641 acgccgatga tgtgtggctg gatgctgaaa gccagcaagc cgcgtgagca aaagcgactg
    47701 cgtggttttg gtcgcatgtt ggtagccctg caacaaggct acggcaagtc gctgaaatgg
    47761 gtgctcaatc atacccgtct ggtgggcgtg gtgctgcttg gcaccattgc gctgaatatc
    47821 tggctatata tctcgatccc gaaaaccttc ttcccggagc aggacactgg cgtgttgatg
    47881 ggcgggatac aggcggatca gagtatttcg tttcaggcga tgcgcggtaa gttgcaggat
    47941 ttcatgaaaa ttatccgtga cgatccggca gtggataatg tcaccggatt taccggcggt
    48001 tcgcgagtga acagcgggat gatgtttatc accctcaagc cacgcgacga acgcagcgaa
    48061 acggcgcagc aaattatcga ccgtctgcgc gtgaagctgg cgaaagaacc gggggcgaat
    48121 ctgttcctga tggcggtaca ggatattcgc gttggcggac gtcagtcgaa cgccagctat
    48181 cagtacacgt tgttatccga cgacctggcg gcactgcgcg aatgggagcc gaaaatccgc
    48241 aaaaaactgg cgacgttgcc ggaactggcg gacgtgaact ccgatcagca ggataacggc
    48301 gcggagatga atctggttta cgaccgcgac accatggcac ggctgggaat cgacgtacaa
    48361 gccgccaaca gtctgttaaa taacgccttc ggtcagcggc aaatctcgac catttaccag
    48421 ccgatgaacc agtataaagt ggtgatggaa gtggatccgc gctataccca ggacatcagt
    48481 gcgctggaaa aaatgttcgt tatcaataac gaaggcaaag cgatcccgct gtcgtatttc
    48541 gctaaatggc aaccggcgaa tgccccacta tcggtgaatc atcagggatt atcggcggcc
    48601 tcgaccattt cgtttaacct gccgaccgga aaatcgctct cggacgccag tgcggcgatc
    48661 gatcgcgcaa tgacccagct tggggtgcct tcgacggtgc gcggcagttt tgccggcacg
    48721 gcgcaggtgt tccaggagac gatgaactcg caggtgatcc tgattatcgc cgccatcgcc
    48781 acggtgtata tcgtgctggg tatcctttac gagagttacg tacatccgct gacgatcctc
    48841 tccaccctgc cctcggcggg cgttggagcg ctgctggcgc tggagctgtt caatgccccg
    48901 ttcagcctaa tcgccctgat agggatcatg ctattaatcg gcatcgtgaa gaaaaacgcc
    48961 attatgatgg tcgattttgc gcttgaagcc caacggcacg gtaacctgac gccgcaggaa
    49021 gctattttcc aggcctgtct gctgcgtttt cgcccgatta tgatgactac cctggcggcg
    49081 ctgtttggtg cgctgccgct ggtattgtcg ggcggcgacg gctcggagct gcggcaaccc
    49141 ctggggatca ccattgtcgg cggactggta atgagccagc tccttacgct gtataccacg
    49201 ccggtggtgt atctcttttt cgaccgtctg cggctgcgtt tttcgcgtaa acctaaacaa
    49261 acggtaaccg agtaaatgac agatcttccc gacagcaccc gttggcaatt gtggattgtg
    49321 gctttcggct tctttatgca gtcgctggac accaccatcg taaacaccgc ccttccctca
    49381 atggcgcaaa gcctcgggga aagtccgttg catatgcaca tggtcattgt ctcttatgtg
    49441 ctgaccgtgg cggtgatgct gcccgccagc ggctggctgg cggacaaagt cggcgtgcgc
    49501 aatattttct ttaccgccat cgtgctgttt actctcggtt cactgttttg cgcgctttcc
    49561 ggcacgctga acgaactgtt gctggcacgc gcgttacagg gcgttggcgg cgcgatgatg
    49621 gtgccggtcg gcagattgac ggtgatgaaa atcgtaccgc gcgagcaata tatggcggcg
    49681 atgacctttg tcacgttacc cggtcaggtc ggtccgctgc tcggtccggc gctcggcggt
    49741 ctgctggtgg agtacgcatc gtggcactgg atctttttga tcaacattcc ggtggggatt
    49801 atcggtgcga tcgccacatt gctgttaatg ccaaactaca ccatgcagac gcggcgcttt
    49861 gatctctccg gatttttatt gctggcggtt ggcatggcgg tattaaccct ggcgctggac
    49921 ggcagtaaag gtacaggttt atcgccgctg acgattgcag gcctggtcgc agttggcgtg
    49981 gtggcactgg tgctttatct gctgcacgcc agaaataaca accgtgccct gttcagtctg
    50041 aaactgttcc gtactcgtac cttttcgctg ggcctggcgg ggagctttgc cggacgtatt
    50101 ggcagtggca tgttgccctt tatgacaccg gttttcctgc aaattggcct cggtttctcg
    50161 ccgtttcatg ccggactgat gatgatcccg atggtgcttg gcagcatggg aatgaagcga
    50221 attgtggtac atgtggtgaa tcgctttggt tatcgtcggg tactggtagc gaccacgctg
    50281 ggtctgtcgc tggtcaccct gttgtttatg actaccgccc tgctgggctg gtactacgtt
    50341 ttgccgttcg tcctgttttt acaagggatg gtcaactcga cgcgtttctc ctccatgaac
    50401 accctgacgc tgaaagatct cccggacaat ctggcgagca gcggcaacag cctgctgtcg
    50461 atgattatgc aattgtcgat gagtatcggc gtcactatcg ccgggctgtt gctgggactt
    50521 tttggttcac agcatgtcag cgtcgacagc ggcaccacac aaaccgtctt tatgtacacc
    50581 tggcttagca tggcgttgat catcgccctt ccggcgttca tctttgccag agtgccgaac
    50641 gatacgcatc aaaatgtagc tatttcgcgg cgaaaaagga gcgcgcaatg aagttctggc
    50701 gacccggtat taccggcaaa ctgtttctgg cgattttcgc cacctgcatt gtcttgctga
    50761 tcagtatgca ctgggcggtg cgtatcagtt ttgagcgtgg ctttattgat tacatcaagc
    50821 atggtaatga acagcgatta caactgttaa gtgatgcgct tggcgagcag tatgcgcagc
    50881 atggcaactg gcgcttcctg cgcaacaatg atcgctttgt ctttcagatc ctgcgttcat
    50941 ttgaacacga taattcggaa gataaacccg gcccgggtat gccaccgcac ggctggcgta
    51001 cccagttctg ggtggttgat caaaacaaca aagtgctggt tggtccgcga gcgccgattc
    51061 cacctgacgg tacacggcga cccattctgg tcaacggtgc ggaagttggc gcggtgatcg
    51121 cctcccccgt tgagcggtta acgcgcaata ctgatatcaa tttcgataaa caacagcggc
    51181 aaaccagctg gttgattgtc gctctggcaa cgttactcgc ggcactcgcc acttttctgc
    51241 tggcgcgcgg tttgctggca ccggtaaaac gacttgtcga tggcacgcac aaactggcgg
    51301 cgggcgattt cactacccgc gtaacgccca ccagtgaaga tgaactgggc aagctggcgc
    51361 aagacttcaa ccagcttgcc agcacgctgg agaaaaacca gcaaatgcgg cgcgatttta
    51421 tggccgatat ttctcacgaa ctgcgtacgc cattagcggt gctgcgcggt gaactggaag
    51481 ccattcagga tggcgtgcgt aaattcacgc cggagacggt ggcgtcttta caggcggagg
    51541 tcggtacact gaccaaactg gttgacgatc tccatcagtt gtcgatgtct gatgaaggcg
    51601 ctctcgccta tcaaaaagca ccggtagatt tgatcccact gctggaagtg gcgggcggcg
    51661 catttcgcga acgattcgcc agtcgtggcc tgaaactgca attttccctg ccagacagta
    51721 ttaccgtatt tggcgatcgc gaccgtttaa tgcagttatt caataactta ctggaaaaca
    51781 gcctgcgcta cactgacagc ggcggcagcc tgcaaatctc tgccggtcag cgcgacaaaa
    51841 cggtgcgcct gacctttgcc gacagtgcgc caggtgtcag tgacgatcag ctacaaaaat
    51901 tgtttgaacg tttttatcgc accgaaggtt cccgcaaccg tgccagcggc ggttccgggc
    51961 tggggctggc gatttgcctg aacattgttg aagcacataa tggtcgcatt attgctgccc
    52021 attcgccttt tggcggggta agcattacag tagagttacc gctggaacgg gatttacaga
    52081 gagaagtatg accgagttac caatcgacga aaacacaccg cgtattttga tcgtggaaga
    52141 tgaaccgaag ctggggcagt tgctcattga ttatctgcgt gctgcgagct atgcgccgac
    52201 gcttatcagc cacggcgatc aggtactgcc gtatgtacgc cagacaccac cggatctgat
    52261 cctgttagat ctgatgctcc ctggcaccga tggcctgacg ctgtgccggg aaattcgtcg
    52321 tttttctgac attccgatcg tgatggtgac ggcaaaaatc gaagagatcg atcgcctgct
    52381 ggggctggag attggcgcag atgattatat ctgtaagccg tacagcccac gggaagtggt
    52441 agcgcgcgtc aaaaccattt tgcgccgttg caaaccgcag cgcgagttgc agcaacagga
    52501 tgctgaaagc ccgttgatta tcgacgaagg tcgttttcag gcttcatggc gcggtaaaat
    52561 gcttgacctg acgcctgcgg aatttcgtct gctgaaaacg ctctctcatg aaccaggaaa
    52621 agtgttctcc cgcgagcatt tgctcaatca tctttatgac gactaccgcg tagtaaccga
    52681 ccgcaccatc gacagccaca ttaaaaacct gcgccgcaag ctggaatctc tcgacgccga
    52741 acagtcattt atccgcgccg tttacggcgt tggttaccgc tgggaagccg acgcctgccg
    52801 catcgtttag ttttagcgac atgactttgt tagccggaga tggcgttcag accagatccg
    52861 gcaacattat cccacgcatg gtcagcaaac tgacattatc tctccgccct ggcaggcatc
    52921 agaaaatcgc tcatacttta atcggtaaac agcaccttta gatgctgttt tcgatacaca
    52981 atttcaatca aggagtcatt atggctggtt ggtttgaact cagtaagagc agtgataatc
    53041 agttccggtt tgtgctaaaa gcgggcaatg gtgagactat cctcaccagc gagctgtata
    53101 cctcaaaagc ctctgcggaa aagggcatcg cgtcggtgcg tagcaacagc ccgcaagaag
    53161 aacgctatga gaaaaaaacg gcaagtaacg gcaaattcta tttcaatctg aaagccgcta
    53221 atcatcaaat tatcggctcc agccagatgt acgccaccgc gcaatctcgt gaaaccggaa
    53281 ttgcctccgt taaagccaat ggcacaagcc agacggtgaa agacaatacg taattacgat
    53341 gccgggcgtg ggttgttggc gtccggcttt atatc
//
LOCUS       DACBVI010000014        52055 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.014, whole genome shotgun sequence.
ACCESSION   DACBVI010000014 DACBVI010000000
VERSION     DACBVI010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.014"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            196..1290
                     /gene="gcvT"
                     /locus_tag="IU325_04285"
     CDS             196..1290
                     /gene="gcvT"
                     /locus_tag="IU325_04285"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HAP5808259.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEQADRDFIGREALEAQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            1314..1703
                     /gene="gcvH"
                     /locus_tag="IU325_04290"
     CDS             1314..1703
                     /gene="gcvH"
                     /locus_tag="IU325_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HAP5808260.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1822..4695
                     /gene="gcvP"
                     /locus_tag="IU325_04295"
     CDS             1822..4695
                     /gene="gcvP"
                     /locus_tag="IU325_04295"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HAP5808261.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVATDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QDGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQN
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREIAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(4861..6294)
                     /locus_tag="IU325_04300"
     CDS             complement(4861..6294)
                     /locus_tag="IU325_04300"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP5808262.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFDWYKEVIASNGENL"
     gene            6339..6650
                     /locus_tag="IU325_04305"
     CDS             6339..6650
                     /locus_tag="IU325_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP5808263.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLETLTEKHAEQENMTLLELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            6814..7473
                     /locus_tag="IU325_04310"
     CDS             6814..7473
                     /locus_tag="IU325_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP5808264.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     IKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(7550..8530)
                     /gene="ygfZ"
                     /locus_tag="IU325_04315"
     CDS             complement(7550..8530)
                     /gene="ygfZ"
                     /locus_tag="IU325_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HAP5808265.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            8773..9039
                     /gene="sdhE"
                     /locus_tag="IU325_04320"
     CDS             8773..9039
                     /gene="sdhE"
                     /locus_tag="IU325_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HAP5808266.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            9020..9427
                     /locus_tag="IU325_04325"
     CDS             9020..9427
                     /locus_tag="IU325_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808267.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9467..9988)
                     /gene="fldB"
                     /locus_tag="IU325_04330"
     CDS             complement(9467..9988)
                     /gene="fldB"
                     /locus_tag="IU325_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HAP5808268.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            10100..10996
                     /gene="xerD"
                     /locus_tag="IU325_04335"
     CDS             10100..10996
                     /gene="xerD"
                     /locus_tag="IU325_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP5808269.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     STLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            11021..11731
                     /gene="dsbC"
                     /locus_tag="IU325_04340"
     CDS             11021..11731
                     /gene="dsbC"
                     /locus_tag="IU325_04340"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HAP5808270.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            11737..13470
                     /gene="recJ"
                     /locus_tag="IU325_04345"
     CDS             11737..13470
                     /gene="recJ"
                     /locus_tag="IU325_04345"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP5808271.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHVGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            13561..14659
                     /gene="prfB"
                     /locus_tag="IU325_04350"
     CDS             join(13561..13635,13637..14659)
                     /gene="prfB"
                     /locus_tag="IU325_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP5808272.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            14669..16186
                     /gene="lysS"
                     /locus_tag="IU325_04355"
     CDS             14669..16186
                     /gene="lysS"
                     /locus_tag="IU325_04355"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP5808273.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(16229..16777)
                     /locus_tag="IU325_04360"
     CDS             complement(16229..16777)
                     /locus_tag="IU325_04360"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP5808274.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRFELGVEITAPEPVYPDF
                     RYRATDPNGIVENEVCPVFAARTTSSLQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQATNREARKRLSAFTQLK"
     gene            complement(16899..17024)
                     /locus_tag="IU325_04365"
     CDS             complement(16899..17024)
                     /locus_tag="IU325_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808275.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(17026..18474)
                     /locus_tag="IU325_04370"
     CDS             complement(17026..18474)
                     /locus_tag="IU325_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP5808276.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            18910..20829
                     /gene="ygfT"
                     /locus_tag="IU325_04375"
     CDS             18910..20829
                     /gene="ygfT"
                     /locus_tag="IU325_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HAP5808277.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPQQVTYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVTIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            20829..21317
                     /locus_tag="IU325_04380"
     CDS             20829..21317
                     /locus_tag="IU325_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAP5808278.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGKN
                     PL"
     gene            complement(21353..22720)
                     /locus_tag="IU325_04385"
     CDS             complement(21353..22720)
                     /locus_tag="IU325_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP5808279.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(22756..24072)
                     /gene="guaD"
                     /locus_tag="IU325_04390"
     CDS             complement(22756..24072)
                     /gene="guaD"
                     /locus_tag="IU325_04390"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HAP5808280.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKELYPEHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(24090..25489)
                     /gene="xanQ"
                     /locus_tag="IU325_04395"
                     /pseudo
     CDS             complement(24090..25489)
                     /gene="xanQ"
                     /locus_tag="IU325_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
     gene            complement(25654..28524)
                     /locus_tag="IU325_04400"
     CDS             complement(25654..28524)
                     /locus_tag="IU325_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HAP5808281.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIDAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(28521..29300)
                     /locus_tag="IU325_04405"
     CDS             complement(28521..29300)
                     /locus_tag="IU325_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HAP5808282.1"
                     /translation="MIEQFFRPDSVEQALELKRRYKDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVTYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(29351..30679)
                     /gene="ssnA"
                     /locus_tag="IU325_04410"
     CDS             complement(29351..30679)
                     /gene="ssnA"
                     /locus_tag="IU325_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HAP5808283.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRNAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATDRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALANGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(30682..33780)
                     /gene="ygfK"
                     /locus_tag="IU325_04415"
     CDS             complement(30682..33780)
                     /gene="ygfK"
                     /locus_tag="IU325_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HAP5808284.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRECLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNKLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDVILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVDSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(34102..34680)
                     /gene="mocA"
                     /locus_tag="IU325_04420"
     CDS             complement(34102..34680)
                     /gene="mocA"
                     /locus_tag="IU325_04420"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HAP5808285.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVKTEYCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNITNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            34783..35553
                     /gene="yqeC"
                     /locus_tag="IU325_04425"
     CDS             34783..35553
                     /gene="yqeC"
                     /locus_tag="IU325_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HAP5808286.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDAPLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHNVEAIWLGDIQEHPAIARRFVN"
     gene            35601..37226
                     /locus_tag="IU325_04430"
     CDS             35601..37226
                     /locus_tag="IU325_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HAP5808287.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDFGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLVTSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFI
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(37267..38199)
                     /gene="arcC"
                     /locus_tag="IU325_04435"
     CDS             complement(37267..38199)
                     /gene="arcC"
                     /locus_tag="IU325_04435"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP5808288.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(38247..39632)
                     /gene="hydA"
                     /locus_tag="IU325_04440"
     CDS             complement(38247..39632)
                     /gene="hydA"
                     /locus_tag="IU325_04440"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HAP5808289.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGSNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNIREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(39685..40896)
                     /locus_tag="IU325_04445"
     CDS             complement(39685..40896)
                     /locus_tag="IU325_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HAP5808290.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(40954..42150)
                     /gene="dpaL"
                     /locus_tag="IU325_04450"
     CDS             complement(40954..42150)
                     /gene="dpaL"
                     /locus_tag="IU325_04450"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HAP5808291.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALNDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(42208..43395)
                     /gene="ygeW"
                     /locus_tag="IU325_04455"
     CDS             complement(42208..43395)
                     /gene="ygeW"
                     /locus_tag="IU325_04455"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HAP5808292.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            43874..45652
                     /locus_tag="IU325_04460"
     CDS             43874..45652
                     /locus_tag="IU325_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HAP5808293.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLALDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(45692..46171)
                     /gene="xdhC"
                     /locus_tag="IU325_04465"
     CDS             complement(45692..46171)
                     /gene="xdhC"
                     /locus_tag="IU325_04465"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HAP5808294.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGMPLSELLRDQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(46168..47046)
                     /gene="xdhB"
                     /locus_tag="IU325_04470"
     CDS             complement(46168..47046)
                     /gene="xdhB"
                     /locus_tag="IU325_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HAP5808295.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRLLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRSVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHVGSAHFKYAMRDAMDISTIGCAAHCRLDSGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(47057..49354)
                     /gene="xdhA"
                     /locus_tag="IU325_04475"
     CDS             complement(47057..49354)
                     /gene="xdhA"
                     /locus_tag="IU325_04475"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HAP5808296.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALADDAEPIHNGGN
                     LLKQSTMTTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTIDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFIEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            49770..50522
                     /locus_tag="IU325_04480"
     CDS             49770..50522
                     /locus_tag="IU325_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP5808297.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSTRKSTAKSTTK
                     TASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYAA
                     GAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGSTD
                     AASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            50601..50674
                     /locus_tag="IU325_04485"
     tRNA            50601..50674
                     /locus_tag="IU325_04485"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50633..50635,aa:Gly,seq:ccc)
     gene            50895..>52055
                     /locus_tag="IU325_04490"
     CDS             50895..>52055
                     /locus_tag="IU325_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808298.1"
                     /translation="MESFMKGKSTLTLLLAGIFSCGTCQATGAEVTSESVFNILNSTG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYM
                     STPDQIKRMQDVISNPKRLDGIPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQND
                     ALGLYLDLLIQAIDTGTINAEDWEKGDRLKSIALLVAYLDKANFYIMEDSGAWEEDAR
                     LNTSSVALVTSGLERLSNLLSKKDSVFISDLLREAKANELDEPLSTTRLNHLIDKGYE
                     RITLQLDLGGESPGYLEKDKHYREADAALLNVIYPANLSKINTRRKEQVLKIV"
BASE COUNT        12774 a        12921 c        13586 g        12774 t
ORIGIN      
        1 gtgattttgg cgttttttaa ccataagcta atgtgatgat caattttacc ttatggttaa
       61 cagtctgttt cggtggtaag ttcaggcaaa agagaacgat tgcgttgggg accgggagtg
      121 gctccgatgc tgggtttcgt ggtgataatt tcgccatgga aaagttgtca gccccgctta
      181 ttcaatgagg acaagatggc acaacagact cctttgtacg aacaacacac gctttgcggc
      241 gctcgcatgg tggatttcca cggctggatg atgccgctgc attacggttc gcaaatcgac
      301 gaacatcatg cggtacgtac cgatgccgga atgtttgatg tgtcacatat gaccatcgtc
      361 gatcttcgcg gcagccgcac ccgggagttt ttgcgttatc tgctggcgaa cgatgtggcg
      421 aagctcacca aaagcggcaa agccctttac tcggggatgt tgaatgcctc tggcggtgtg
      481 atagatgatc tcatcgtcta ctactttact gaagatttct tccgcctcgt tgttaactcc
      541 gccacccgcg aaaaagacct ctcctggatt acccaacacg ctgaaccttt cggcatcgaa
      601 attaccgttc gtgatgacct ttccatgatt gccgtgcaag ggccgaatgc gcaggcaaaa
      661 gctgccacac tgtttaatga cgctcagcgt caggcggtgg aagggatgaa accgttcttt
      721 ggcgtgcagg cgggcgatct gtttattgcc accactggtt ataccggtga agcgggctat
      781 gaaattgcgc tgcccaatga aaaagcggcc gatttctggc gtgcgctggt ggaagctggc
      841 gttaagccat gcggcctggg cgcgcgtgac acgctgcgtc tggaagcggg tatgaatctt
      901 tatggtcagg agatggacga aaccatttct ccgttagccg ccaacatggg ctggactatc
      961 gcctgggaac aggcagatcg tgactttatt ggtcgtgaag ctctggaagc gcagcgtgaa
     1021 catggtacag aaaaactggt tggtctggtg atgaccgaaa aaggcgtgct gcgtaatgaa
     1081 ctgccggtac gttttaccga tgcgcagggc aaccagcatg aaggcattat caccagcggg
     1141 actttctccc cgacgctggg ttacagcatt gcgctggcgc gcgtgccgga aggtattggc
     1201 gaaacggcga ttgtgcaaat tcgcaaccgt gaaatgccgg ttaaagtgac gaaacctgtt
     1261 tttgtgcgta acggcaaagc cgtcgcgtga tttacttttt tggagattga ttgatgagca
     1321 acgtaccagc agaactgaaa tacagcaaag aacacgaatg gctgcgtaaa gaagccgacg
     1381 gcacttacac cgttggcatc accgaacacg ctcaggagct gttaggcgat atggtgtttg
     1441 ttgacctgcc ggaagtaggc gcaacggtta gcgcgggcga tgactgcgcg gttgctgaat
     1501 cagtaaaagc ggcgtcagac atttatgcgc cagtaagcgg tgaaatcgtg gcggtaaacg
     1561 acgcactgag cgattccccg gaactggtga acagcgaacc gtatgcaggt ggctggatct
     1621 ttaaaatcaa agccagcgat gaaagcgaac tggaatcact gctggatgcg accgcatacg
     1681 aagcattgtt agaagacgag taacggcttt attcctcttc tgcgggagag gatcagggtg
     1741 aggaactttt acgcctcacc ctcactctct tcgcaaggag agaggttcac aattcactgc
     1801 acgtttcagg aaccatcgct catgacacag acgttaagcc agcttgaaaa cagcggcgct
     1861 tttattgaac gccatatcgg accggacgcc gcgcaacagc aagaaatgct gaatgccgtt
     1921 ggcgcacaat cgttaaacgc gctgaccggc cagattgtgc cgaaagatat tcagcttgcg
     1981 accccgccgc aggttggcgc accggcgacc gaatacgccg cgctggcgga actcaaggct
     2041 attgccagtc gcaataaacg cttcacgtct tacatcggca tgggttacac cgccgtgcag
     2101 ctaccgccgg ttattctgcg taacatgctg gaaaatccgg gctggtatac cgcgtatact
     2161 ccgtatcaac ctgaagtctc ccagggccgc cttgaagcac tgctcaactt ccagcaggta
     2221 acgctggatt tgactggact ggatatggcc tctgcttctc ttctggacga ggccaccgct
     2281 gccgccgaag caatggcgat ggctaaacgc gtcagcaaac tgaaaaatgc caaccgcttc
     2341 ttcgttgcta ctgacgtcca tccgcaaacg ttggatgtgg tacgtactcg tgccgaaacc
     2401 tttggttttg aagtgattgt cgatgacgcg caaaaagtgc tcgaccatca ggacgtcttc
     2461 ggcgtgctgt tacagcagga tggcacaacc ggtgaaatcc acgactacac tgcgctcatc
     2521 agcgaactga aatcacgcaa aattgtggtc agtgtcgccg ccgatattat ggcgctggtg
     2581 ctgttaactg cgccgggtaa acagggcgcg gatattgttt ttggttcggc gcaacgcttt
     2641 ggcgtaccga tgggttacgg tggcccacac gcggcattct ttgcggcgaa agatgaatac
     2701 aaacgctcaa tgccgggccg tattatcggt gtatcgaaag atgcagctgg caacaccgct
     2761 ctgcgcatgg cgatgcagac tcgcgagcaa catattcgtc gtgagaaagc gaactccaac
     2821 atttgtactt cccaggtgct gctggcaaac atcgccagcc tgtatgccgt ttatcacggc
     2881 ccggtgggcc tgaaacgtat cgctaaccgc attcaccgtc tgaccgatat cctggcggcg
     2941 ggcctgcaac aaaaaggtct gaaactgcgt catgcgcact atttcgatac tttgtgtgtg
     3001 gaagtggctg acaaagcggg cgtgctggcg cgtgccgaag cggctgaaat caacctgcgt
     3061 agcgatattc tgaacgcggt tgggatcacc cttgatgaaa ccaccacgcg tgaaaacgtg
     3121 atgcagcttt tcagcgtgct gttgggcgat aaccacgggc tggacatcga cacgctggac
     3181 aaagacgtgg ctcacgatag ccgctctatt cagcctgcaa tgttgcgcga cgacgaaatc
     3241 ctcacccatc cggtgtttaa tcgctaccac agcgaaaccg aaatgatgcg ctatatgcac
     3301 tcgctggagc gtaaagatct ggcgctgaat caggcgatga tcccgctggg ttcctgcacc
     3361 atgaaactga acgccgccgc cgagatgatc cccatcacct ggccggaatt tgccgaactg
     3421 cacccgttct gcccgccgga acaggccgaa ggttatcagc agatgattgc gcaactggct
     3481 gactggctgg tgaaactgac cggttacgac gccgtttgta tgcagccgaa ctctggcgca
     3541 cagggcgaat atgcgggcct gctggcgatt cgtcattatc atgaaagccg caacgaaggg
     3601 catcgcgata tctgcctgat cccggcttct gcgcacggaa ctaaccccgc ttctgcacat
     3661 atggcaggaa tgcaggtggt agttgtggca tgtgataaaa atggcaacat cgatctgact
     3721 gacctgcgcg cgaaagcgga acaagcgggc gataatctct cctgcatcat ggtgacttat
     3781 ccttccaccc acggcgtgta cgaagaaacg atccgtgaag tgtgtgaagt cgtgcatcag
     3841 ttcggcggtc aggtttacct tgatggcgcg aacatgaacg cccaggtcgg catcacctca
     3901 ccgggcttta ttggcgcgga cgtctcgcac cttaacctgc ataaaacttt ctgcattccg
     3961 cacggtggcg gcggccctgg tatgggaccg atcggcgtga aagcgcatct ggcaccgttt
     4021 gtgccaggtc atagcgtggt acaaattgaa ggcatgttaa cccgtcaggg cgcggtttct
     4081 gcggcaccgt tcggtagcgc atccattctg ccaatcagct ggatgtacat ccgcatgatg
     4141 ggcgcagaag ggctgaaaaa agcaagccag gtggcaattc ttaacgccaa ctatattgcc
     4201 agccgcctgc agaatgcttt cccggtgctg tataccggtc gcgacggtcg cgtggcgcac
     4261 gaatgtattc ttgatattcg tccgctgaaa gaagaaaccg gcatcagcga actggatatt
     4321 gccaagcgtc tgatcgacta cggtttccac gcgccgacca tgtcgttccc ggtggcgggt
     4381 acgctgatgg ttgaaccgac tgaatctgaa agcaaagtgg aactggatcg ctttatcgac
     4441 gcgatgctgg ctatccgcgc agaaatcgac caggtgaaag ccggagtctg gccgctggaa
     4501 gataacccgc tggtgaacgc gccgcacatt cagagcgaac tggtcgccga gtgggcgcat
     4561 ccgtacagcc gtgaaattgc ggtattcccg gcaggcgtgg cagacaaata ctggccgaca
     4621 gtgaaacgtc tggatgatgt ttacggcgac cgtaacctgt tctgctcctg cgtaccgatt
     4681 agcgaatacc agtaatttac tgattcgact atcttctaaa ggcgcttcgg cgccttttta
     4741 gcttgttaat aaagtgcgct ttgtgtgttt cggctgaggc gtaatataaa atattgcgag
     4801 ctcaatatat taaaagagtg ccaggcctga tgagcgtagc gcatcaggca catcggctaa
     4861 ttacagattc tcgccgttgc tggcaatcac ctctttgtac cagtcaaagc tcttcttacg
     4921 tgaacgcgac atatcgccag taccgtcgtc atgtttattc acatagataa agccgtagcg
     4981 tttgctgtac tgcccggtag tgaacgacac gcaatcgatg cagccccacg gtgtgtagcc
     5041 catcagatcc acgccatcgt aagtcaccgc ttttttcatc tcttcaatat gggcgcgcag
     5101 gtagtcaatg cggtagtcat cgttgatgct gccatcttct tccactttgt cataagcgcc
     5161 aaaaccgttt tcgacaataa acagcggctt ctgataacgc tcatacagtt cgcaaagtgc
     5221 atagcgcaga cccacaggat caatctgcca gccccagtca gacgctttaa catacgggtt
     5281 tggtacgctg ccttcaaaac cagagatcgc atcgccggtg ccgccttcgg ccttcaccgc
     5341 gttggtcatg taatagctga aaccgagata atcgcaggtg ccttcgcgca gcacatcgag
     5401 atcgccgtct tccattttga tgttaaatcc gcgacgctcc cactcgttca acacatagga
     5461 cgggtaatag ccacgcagtt gcacatcggt aaagacgtag cgttcgcgca tcgactcttg
     5521 ggcgaacatc acatcgtccg gattacagga gtaagggtaa agcggcacca tcgccagcat
     5581 acagccgact ttcatttccg ggttaatgcg acgcgcggct ttcaccgcca gggcgctggc
     5641 gacaaactgg tgatgcagca cctgatacat cgtctcttcc gggttttcat gctcggtata
     5701 caccacgccg gagcagcagt aaccgaacag cggtgcacgc cagttacgct ggttgttaat
     5761 ttcgttgaag gtcatccagt atttgacttt atgcttatag cgttcaaata ccacttctgc
     5821 gaaacggaca aagaaatcaa ccactttacg gttggtccag ctaccgtatt gctgcaccag
     5881 atgcagcggc atttcaaagt gggagagggt gatcaccggt tcgatgttgt atttcagcag
     5941 ttcatcgaac atatcatcgt agaacttcag cccttcttcg tttggctgag cttcatcgcc
     6001 ttttggaaaa atgcgcgtcc aggcgatgga tgtacgaaaa catttgaagc ccatttcggc
     6061 aaatagcttg atgtcttcct tgtaatgacc ataaaaatca acggcttcat ggttcggata
     6121 gtattttcct ggcaacactt ctttggtgat ttcgcgcggc acgccgtgtg cgccaccggt
     6181 cagaacgtca caaatgctcg gccctttgcc gcctttgttc cagccgcctt cgacctgatg
     6241 agcggcaact gcgccgcccc ataagaaatc tttcggtaag gtgagttttt tcactatcat
     6301 aggctccaga tgagtcaatt cttacaggaa atagtaccat gcagccaaac gacatcactt
     6361 tttttcaacg tttccaggat gacattctgg ctgggcgtaa aaccatcacc atccgcgacg
     6421 agtctgaatc gcacttcaaa acgggtgatg tgcttcgtgt cgggcgtttt gaagatgacg
     6481 gttatttttg cacgattgaa gtcaccgcaa cctcaaccgt aacgctggag acgctgacgg
     6541 aaaaacatgc agagcaggag aatatgaccc tgctagaact gaaaaaggtc attgccgaca
     6601 tctatcccgg ccagacacag ttttatgtga ttgaatttaa atgcctttaa attgaactca
     6661 cgcctgttag ttgaaatttt gcatttaatt tcatgatttt taggagatta gtcccaaatc
     6721 agaatttatt ttagctaaca ggtgttcact ggaactattc tcagttacgc tggagaggta
     6781 tacatgtgcg tgtacgtttc tccggagtaa gttatggttc agaagcccct cattaagcag
     6841 ggatattcgc tggcagagga aattgccaac agcgtcagtc acggcattgg gttggtgttt
     6901 ggtatcgttg ggctggtgtt gctactggtt caggcggtgg atctcaatgc cagcgccaca
     6961 gcgataacca gttacagcct ctatggcggc agtatgatcc tgctgttcct cgcttcgacg
     7021 ctctatcacg ccattcctca tcaacgggca aaaatgtggc tgaagaaatt tgaccattgc
     7081 gctatttacc tattgattgc cggaacctac acgccgtttt tgctggtggg gctggattct
     7141 ccgttagcgc gcgggttgat gattgttatc tggagcctgg cattgctggg gattctgttc
     7201 aaactgacca tcgcgcaccg attcaaaatt ttatctctgg tgacctatct ggcgatgggc
     7261 tggctgtcgc tggtggtgat ttatgaaatg gcaattaagc ttgcggcggg cagcgttacc
     7321 ttactggctg taggcggcgt ggtttactcg ctcggggtga ttttctacgt ctgcaaacgc
     7381 attccctaca accatgccat ctggcacggc tttgtgctcg gcggcagcgt gtgccacttt
     7441 ctggcgatct atttatatat tgggcaggcg taatgggtgt gcctttgtag gcctgaaaag
     7501 acacaccctt gtcgcatcag gcgataaatg catgatgcga cacagagatt tactcttcga
     7561 gtgaatacgg cagcggctcg atatgcaatg tattcgcatc gtcacgcacg cggaagatgc
     7621 tgtccggttc catatcgtta ttcatgacga cctgtaccac gacctgacca tcttccagtt
     7681 ttaccgcagc cagcacggtt ccggtacgac gccagttctc gcccattttc agctctaagt
     7741 cttcaccggc ttccggcagt cggctggcgc tacctgtcag caaccagagc gcgcgtttgt
     7801 tggcaccacg gaatttagct cgcgccacca tctcttgtcc ggtatagcag ccttttttaa
     7861 agctaatgcc gcccagcgcc tggaggttgg tcgcctgtgg gataaactgc ccgctgttgg
     7921 cggcatcaat caccgggaaa cccgcttcaa tgtttaatgc cagccactgt tggctattgt
     7981 tcagttccgc ttcaccgcgc agtttatcgg tcagcatatt ggcagtagct tcatcggtta
     8041 cgatcaggaa acgttctgcc gggtgttcga accatagcaa agtggtcgcg ccttctttaa
     8101 ctacctgttt ttctttcgaa ggcagttcac taaagatatt tgccagcgcg gcgcgcgcct
     8161 gaaaaccggc aacaccaagc agcacacgct catcgtccgg cgcgatggtc actttagaga
     8221 acaccgcata ttttttcagt tcagtcagct gcggttcacg cacgctgcgc cgttcaatcc
     8281 atgcaaagcc atcgccgtcg cggaacagac gtaaattgct ccacattttg cctttggcgt
     8341 cgcaatgggc ggcgagcagg tgctgatctt ctgtcatctg gctgacatct gctgtcacct
     8401 gaccctgcat atatttttcg ctgtccgcgc cagtaatggt ggcaagcgcc cagtcatcaa
     8461 gcgtcatcag cgtcagtggc aaacgggcag aagccgtagg ctgacgggga ggaaaaggtg
     8521 taaaagccat aatagagtcc tgagttgctt aacggcgtat taatgactaa tggtaaaaga
     8581 gctatgaggc aatgcaagtg ctttcactac gcgatctgtg ccttctgcga ggcgacttcc
     8641 aaaaaaatga agcccttgaa ttgttgtgaa ttaatttcgc gatcgcgatt gtgtttactg
     8701 atattcccgc aaaagaagtc gaaaaacacg ttacaatagc ggggtactgg attttcgtga
     8761 gacaggaaga agatggacat taacaacaaa gcccgcattc attgggcatg ccgccgtggt
     8821 atgcgcgaac tcgatatttc aatcatgccg tttttcgaac atgagtacga cagcttaagc
     8881 gatgacgaaa aacgcatctt tattcgtctg ctggaatgtg acgatcccga tctgtttaac
     8941 tggctgatga atcacggtaa accggccgat gcagaactgg aaatgatggt ccgactcatc
     9001 cagacacgga accgggaacg tggtcctgtg gcaatctgat ttgcgcgtct cctggcgcgc
     9061 acagtggctt tccttgctga tccacgggct ggttgccgct gttattttac tcatgccctg
     9121 gccgctcagt tacaccccgt tatggatggt gttactttcg ctggtggtgt ttgattgcgt
     9181 tcgcagccag cgacgcatta atgctcgcca gggagaaatt cgcctgttga tggacgggcg
     9241 tttgcgctgg caagggcagg agtggagcat cgtcaaagcg ccgtggatga ttaagagcgg
     9301 catgatgctg cgtttacgtt ctgacagcgg taaacggcaa catttatggc tggcagccga
     9361 cagcatggac gaagccgaat ggcgggattt acggcggatt ttgttgcaac aagagacgca
     9421 aagataaaga tgacggcaaa gcatatgcgc taagccgtca gtgacatcag gcgtaatgct
     9481 ctgccatttc gttgaggatt tgttcacacc agctctgaat acgctcgtcg ctaaggtcat
     9541 actggttagt ttcgtccagc gccagcccca cgaacagttg cccgtcagca atcaccggtt
     9601 tcgggctggt aaattcatat ccttccgttg gccagtagcc gacgaacttc acgcctttgg
     9661 tcgagagttt gtcatgcagc ataccgagcg catcgaggaa ccactcgccg tatcccagtt
     9721 gatcgccaag cccatacagc gcaacaattt taccttcgag gttcaggtcg tcgagctgat
     9781 cccagacggc ttcccagtct tcctggattt cgccaaaatc ccaggtcggg atacccagaa
     9841 tgagcacatc gtactgctcc attaatttcg gggagtcgtc cttgaggtta tgtaaggtca
     9901 ccagttctgg gccgataata tcgcggattt tttctgccgc catttcggtg taacaggtgc
     9961 tggaaccgta aaaaagaccc atattcataa cgtaagcttc tcgattctgc tgtggctttt
    10021 gcggctagtg tatcagaatc gctttagtca cggcacaata aggcataatg gatcaaatga
    10081 gtgaccataa ggggcgcaag tgaaacagga tctggcacgc atcgagcagt ttcttgatgc
    10141 tctgtggctg gaaaaaaatc tggctgaaaa tacgttgaac gcataccgtc gcgatctgtc
    10201 aatgatggtg gagtggttgc atcaccgcgg gtcgacgctg gcgacggcgc aaagtgacga
    10261 tttgcaggca ttactggcag aacggctgga gggcgggtat aaagccacca gctcagcgcg
    10321 tttgctgagt gcagtgcgtc gattgttcca gtatctttat cgcgaaaagt ttcgtgaaga
    10381 cgatcccagt gcgcatctcg cttcaccgaa attgccacag cgtttgccaa aagatttaag
    10441 cgaagcgcag gtcgaacgtt tattacaggc accattaatt gatcagccac tggagctacg
    10501 cgataaagcc atgcttgaag tgttgtatgc taccggactg cgtgtctctg aactggtcgg
    10561 gctgacaatg agtgatatca gcctgcgtca gggcgtagtg cgggtcattg gtaaaggcaa
    10621 caaagagcgc ctggtgccgt taggtgaaga ggcggtttac tggttggaga cctatctgga
    10681 acatgggcgt ccgtggttgt tgaacggtgt ctctattgat gtgttgttcc ccagccagcg
    10741 tgcgcagcag atgacgcgac agaccttctg gcaccgcatt aaacattatg ccgtgctggc
    10801 gggtatcgac agcgaaaagc tgtcaccgca tgtgttgcgt cacgcttttg ccactcattt
    10861 attaaatcat ggtgcggatt tacgcgtggt gcagatgcta ctgggccaca gcgatctctc
    10921 caccacgcaa atttatacgc atgtcgctac cgagcgtctg cggcaacttc atcaacagca
    10981 tcacccgcgg gcgtgatgtc tgaaaagaac gggaagattt atgaagaaag gttttatgtt
    11041 gtttactttg ttagcggcgt tttcaggctt tgctcaggct gatgacgcgg caattcaaca
    11101 aacgttagcc aaaatgggca tcaaaagcag cgatattcag cccgcgcccg tagctggcat
    11161 gaagacagtt ctgactaaca gcggtgtgtt gtacatcacc gatgatggta aacatatcat
    11221 tcaggggcca atgtatgacg ttagtggcac agctccggtc aatgtcacca ataagatgct
    11281 gttaaagcag ttgaatgcgc ttgaaaaaga gatgatcgtt tataaagcgc cacaggaaaa
    11341 acacgtcatc accgtgttta ctgatattac ctgtggttac tgccacaaac tgcatgagca
    11401 aatggcagac tacaacgcac tggggatcac cgtgcgttat cttgctttcc cgcgccaggg
    11461 gctggacagc gatgcagaga aagaaatgaa agctatctgg tgtgcgaaag ataaaaacaa
    11521 agcgtttgat gatgtaatgg caggtaaaag cgtcgcacca gccagctgcg acgtggatat
    11581 tgccgaccat tacgcacttg gcgtccagct tggcgttagc ggtactccgg cagttgtgct
    11641 gagcaatggc acacttgttc cgggttacca gccaccgaaa gacatgaaag aattcctcga
    11701 cgaacaccaa aaaatgacca gcggtaaata attcgcgtga aacaacagat acaacttcgt
    11761 cgccgtgaag tcgatgaaac ggcagacttg cccgctgaat tgcctccctt gctgcgccgt
    11821 ttatatgcca gccggggtgt gcgtagtgcg caagaactgg aacgcagtgt taaaggtatg
    11881 ctgccctggc agcaactgag cggcgtcgaa aaggccgttg agatccttta caacgccttt
    11941 cgcgaaggaa cgcggattat tgtggtcggc gatttcgacg ccgacggagc caccagcacg
    12001 gctctaagcg tgctggcgat gcgctcgctt ggttgcagca atatcgacta cctggtacca
    12061 aaccgtttcg aagacggtta cggcttaagc ccggaagtgg tcgatcaggc ccatgcccgt
    12121 ggcgcgcagt taattgttac cgtggataac ggtatttcct cccatgtggg ggttgaacac
    12181 gctcgctcgt tgggcatccc ggttattgtt accgatcacc atttgccggg cgacacatta
    12241 cccgcagcgg aagcgatcat taaccctaac ttgcgcgact gcaatttccc gtcgaaatca
    12301 ctggcaggcg tgggtgtggc gttttatctg atgctggctc tgcgcacctt tttgcgcgat
    12361 cagggctggt ttgatgagcg cggcatcgca attcctaatc tggcagaact gctggatctg
    12421 gtcgcgctgg ggacagtagc ggacgtcgtg ccgctggacg ctaataatcg cattctgacc
    12481 tggcagggga tgagtcgcat ccgtgccgga aagtgccgtc cggggattaa agcgctgctg
    12541 gaagtggcaa accgtgatgc gcaaaaactc gccgccagcg atttaggttt tgcgctgggg
    12601 ccacgtctca atgctgccgg gcgactggat gatatgtccg ttggtgtggc gctgttgctg
    12661 tgcgacaaca tcggtgaagc gcgcgtgctg gcaaatgaac tcgatgcgct aaaccagacg
    12721 cgaaaagaga tcgaacaagg aatgcaaatt gaagccctga ccctgtgcga gaaactggag
    12781 cgcagccgcg acacgctacc cggcgggctg gcaatgtatc accccgaatg gcatcagggg
    12841 gttgtaggca ttctggcttc ccgtattaaa gagcgttttc accgtccggt tatcgcgttt
    12901 gcgccagcag gtgacggtac gctgaaaggt tctggtcgtt ccattcaggg gttgcatatg
    12961 cgcgatgcgc tggagcggct ggacacgctc taccccggta tgatgcttaa gtttggcggc
    13021 catgcaatgg cggccgggct gtcactggaa gaggataaat tcgaactctt tcagcaacgc
    13081 tttggcgaac tggtgactga gtggctggac ccttcactat tgcaaggcga agtggtgtcg
    13141 gacggtccgt taagcccggc cgaaatgacc atggaagtgg cgcagttgct gcgcgatgct
    13201 ggcccgtggg ggcagatgtt cccggagccg ctgtttgacg gtcatttccg tctgctgcaa
    13261 cagcggctgg tgggcgaacg tcatttgaag gtgatggtcg aaccggtcgg cggcggtccg
    13321 ctgctggatg gtattgcttt caatgtcgat accgccctct ggccggataa cggcgtgcgc
    13381 gaagtgcaac tggcttacaa gctcgatatc aacgagtttc gcggcaaccg cagcctgcaa
    13441 attatcatcg acaatatctg gccaatttag cgtcatcttc tctataaaaa agagcgtgga
    13501 ttgggtacaa tcccgctctt atcaccgcat tttgactagc tcaataaaag aaatcagacc
    13561 atgtttgaaa ttaatccggt aaataatcgc attcaggacc tcacggaacg ctccgacgtt
    13621 cttagggggt atctttgact atgacgccaa gaaagagcgt ctggaagaag taaacgccga
    13681 gctggaacag ccggatgtct ggaacgaacc cgaacgcgca caggcgctgg gtaaggagcg
    13741 ttcctccctc gaagccgttg tcgataccct cgaccaaatg aaacaggggc tggaagatgt
    13801 ttctggtctg ctggaactgg ctgtagaagc tgacgacgaa gaaaccttta acgaagccgt
    13861 tgctgaactc gacgctctgg aagaaaaact ggcgcagctt gagttccgcc gtatgttctc
    13921 tggcgaatat gacagcgccg actgctacct cgatattcag gcggggtctg gcggtacgga
    13981 agcacaggac tgggcgagca tgcttgagcg tatgtatctg cgctgggcag aatcgcgtgg
    14041 tttcaaaact gaaatcatcg aagagtcgga aggtgaagtg gcgggtatta aatccgtgac
    14101 gatcaaaatc tccggcgatt acgcttacgg ctggctgcgt acagaaactg gcgttcaccg
    14161 cctggtgcgt aagagcccgt ttgactccgg cggtcgtcgc cacacgtcgt tcagctccgc
    14221 gtttgtttac ccggaagttg atgacgatat cgatatcgaa atcaatccgg cggatctgcg
    14281 catcgacgtt tatcgcgcgt ccggcgcggg cggtcagcac gttaaccgta ccgaatctgc
    14341 ggtgcgtatt actcacatcc caactgggat cgtgacccag tgccagaacg accgttccca
    14401 gcacaagaac aaagaccagg ccatgaagca gatgaaagcg aagctttatg aactggagat
    14461 gcagaagaaa aatgccgaga agcaggcgat ggaagataac aaatccgaca tcggctgggg
    14521 cagccagatt cgttcttatg tccttgatga ctcccgcatt aaagatctgc gtaccggggt
    14581 agaaacccgt aacacgcagg ccgtgctgga cggcagcctg gatcaattta tcgaagcaag
    14641 tttgaaagca gggttatgag gaaccaacat gtctgaacaa cacgcacagg gcgctgacgc
    14701 ggtagtcgat cttaacaatg aactgaaaac gcgtcgtgag aagctggcga acctgcgcga
    14761 gcaggggatt gccttcccga acgatttccg tcgcgatcat acctctgacc aattgcacgc
    14821 agaattcgac ggtaaagaga acgaagaact ggaagcgctg aacatcgaag tcgccgttgc
    14881 tgggcgcatg atgacccgtc gtattatggg taaagcgtct ttcgttaccc tgcaggacgt
    14941 ggggggccgc attcagctgt acgttgcccg tgacgatctg ccggaaggcg tttacaacga
    15001 gcagttcaaa aaatgggacc tcggcgacat cctcggcgcg aaaggtaaac tgttcaaaac
    15061 caaaactggc gaactgtcta tccactgcac cgagctgcgt ctgctgacca aagcactgcg
    15121 tccgctgccg gacaaattcc acggcttgca ggaccaggaa gcgcgctatc gtcagcgtta
    15181 tctggatctc atctctaacg atgaatcccg caacaccttt aaagtgcgct cgcagatcct
    15241 ctctggtatt cgccagttca tggtgaaccg cggctttatg gaagttgaaa cgccgatgat
    15301 gcaggtgatc cctggcggtg ccgctgcgcg tccgttcatc acccaccata acgcgctgga
    15361 tctcgacatg tacctgcgta tcgcgccgga actgtacctc aagcgtctgg tggtcggtgg
    15421 cttcgagcgt gtattcgaaa tcaaccgtaa cttccgtaac gaaggtattt ccgtacgtca
    15481 taacccagag ttcaccatga tggaactcta tatggcttac gcagattaca aagatctgat
    15541 cgagctgacc gaatcgctgt tccgtactct ggcacaggat attctcggta agacggaagt
    15601 gacctacggc gacgtaacgc tggacttcgg taaaccgttc gaaaaactga ccatgcgtga
    15661 agcgatcaag aaatatcgcc cggaaaccga catggcggat ctggacaact tcgactctgc
    15721 gaaagcgatt gctgaatcta tcggcatcca cgttgaaaag agctggggtc tgggccgtat
    15781 cgttaccgag atcttcgaag aagtggcaga agcacatctg atccagccga ccttcattac
    15841 tgaatatccg gcagaagttt ctccgctggc gcgtcgtaac gacgttaacc cggaaatcac
    15901 agaccgcttt gagttcttta tcggtggtcg tgaaatcggt aacggcttta gcgagctgaa
    15961 tgacgcggaa gatcaggcgc agcgcttcct ggatcaggtt gccgcgaaag acgcaggcga
    16021 cgacgaagcg atgttctacg acgaagatta tgtcaccgca ctggaacacg gcttaccgcc
    16081 gacagcaggt ctgggaattg gtatcgaccg tatggtaatg ctgttcacca acagccatac
    16141 catccgcgac gttattctgt tcccggcgat gcgtccggtg aaataagtat tacgttatgc
    16201 tcacaacccc ggcaaatgtc ggggtttttt atttaagctg ggtaaaagca gataatcgtt
    16261 ttctggcttc gcgatttgtc gcctgcatca ccatccacgg actgaacgcc cacggtgtgg
    16321 catcaatacc gtgtaataca tctgctaaat cacaccattg ataatccatc acttcatcat
    16381 cgttgatctg taacgaactg gtcgtgcgtg cagcaaatac cggacacact tcattttcca
    16441 caatgccatt cggatcggtg gcgcggtagc gaaagtcagg atagaccggt tctggtgctg
    16501 ttatttccac acccagttca aagcggcaac ggcggatcac cgcgtcttcg ttgctttcgc
    16561 ccagttgtgg gtgcccacaa accgagttag tccacacgcc cggccacgct tttttgctaa
    16621 gggcgcggcg ggtaactaat aattgccctt tggcattaaa cagccaactg gagaacgcga
    16681 gatgtaagag agtgtctgcc gtgtgtgcgg catacttttc cagcgtaccc gtgggaactc
    16741 cctgtgcatt caataaaatg acgtgttccg tttgcataat ttctcacatg taattctaat
    16801 cactcatcat tatagataat tgaagagtga aatgattaac caagtgagcg tttatcgaca
    16861 accgcccgtt ttgagcggat gtcgacaggt aaaaccattt aaaaaagcag catcatatat
    16921 ttgaagcgac tttcaatggc cattgcaaca cagtcagaaa tgacaggaat agagagagta
    16981 aaaagcaaca acagactgaa tatagcgcgc attaaaagat tcatattatt ctccatgctc
    17041 atttttcttc agcttgcgca tcagaagcca catacgtact gtcctgactt ttaaatcttt
    17101 atcagagacg gattccttca ccagatcagc atgatgctga tagccattaa agaagacatt
    17161 cagcacaacg gcgctaaggg ttgcgagcat aatgccgcta tgcagcaacg gttgcagtac
    17221 ggccggtaac ttagaaaaga aatcgtgaga gagcgtcgga gtcatgccaa cgccgagact
    17281 gatcgccaca atatagaggt tgtaacggtt agtggtgtag ttacagcgcg acagaattcg
    17341 aatccctgtc gccagtacca tgccgaacat caccagccca gcaccgccca gcacaaattg
    17401 cggaatggag gcgaccagca ccgccatttt tggcaccatg ccaaacagga ttaatataat
    17461 tcccgaagaa atacacaccc agcggctatg aacgcgcgtc acgctaacca ggccaacgtt
    17521 ttgagaaaat gacgtgtggg ggaagctgtt aaacgttccg cctatcattg tccctacgcc
    17581 atcgacacgc agcccgcgaa taatatcgtg tgaagagagt ttacgaccga ctatttcacc
    17641 cagtgccagg aacatcccca tcgactcgat aaacacgatg attaacacgg cagtcatggt
    17701 cagaatggaa acgggatcga aaatcggcat accaaatgac atcggcgtga caatcgcaaa
    17761 ccatgaagca tcatgtagcc cggataaatt gacttcattc atcatccacg aaagtaaaaa
    17821 gccaaataca atccccagta atacggcgac gttggacata aatcctttcg catagcgagt
    17881 aatgagcaag ataaaaatta agacggcaaa ggagatacct aaataaacgg gattaccata
    17941 ttgcggattc cctttacctc ctgcggccca gtcaataccc acctgaatga tgctaagtcc
    18001 gatagaagta ataaccacac cggtaaccag tggcgggaat aaaggcatca agcgaccgat
    18061 aagtggcgct aataatgtgg tgataaaacc cgcggcgata gtggcaccaa atatccccag
    18121 caggccgata tccgggttca tcccaatggc tatcattggt gttacagcgg cgaaggtcac
    18181 cgacataatc accggcaggc ggatccccat aaagcggccg ataccgatac attgcaataa
    18241 tgtgacgatc ccgcagcaaa agagatccga gctaatgagc atcgcaatag cttcttttga
    18301 gaggcccagt cggtcaccaa tcataagagg aacagcgact gcacctgcgt acatgacaag
    18361 gacgtgttgc agaccgagta tgatcagctt tcctggtgat aatatgcggt caacctcatc
    18421 agttgggcgg tcttgccctg aagatgaggg aagttgggaa tctatggcgc tcatcagtgt
    18481 ttctcctttc gcccgccaga gtaaaaagca accgtttacg ttaatgaaac tgtctaaaca
    18541 catgctcgtg gagtgccagt ttttgcgagg aaggggagtt agagacagga attgcggagg
    18601 caatcacata atcgtggtta aaccacgaaa atgagaagag aacctgacaa tggaattatc
    18661 tgtaattttc tcatattgat atttcattaa tcattctggc atgtcgcact ctcgcattta
    18721 atcgttttta tctggatagc gctcttttgg tcggcaatgt tgaaataatg aaagcggcac
    18781 agatgaaggg gcaataattt gaactcagtc gcaaaattat aattaaaacg gtcatgctac
    18841 ctaatatatg cccgtttatt gcatcgcgaa atttgagaag acataactca ttaaatgaaa
    18901 gggatgcaaa tgaataagtt tatcgctgct gaagctgcgg aatgtatagg ctgtcatgct
    18961 tgtgaaattg cctgtgcggt ggcacataat caagaaaact ggccgctgag tcacagtgac
    19021 tttcgaccgc gtatccacgt tgtagggaaa ggccaggctg cgaatccggt ggcctgccat
    19081 cactgcaaca atgccccttg cgttacggct tgtccggtta atgctctgac tttccagtcc
    19141 gatagcgtac aactggacga gcaaaaatgt attggttgta aaagatgcgc aatcgcttgc
    19201 ccctttggcg tcgttgagat ggtcgatacg attgcgcaga aatgcgacct ttgtaaccag
    19261 cgcagctccg gcacgcaagc ctgtattgaa gtctgcccaa cacaggcgtt acggctgatg
    19321 gacgataaag ggttgcagca gataaaggtg gcccgccagc gcaaaacggc agcaggaaaa
    19381 gcgtcatcag acgctcagcc atctcgcagt gcagcgttgc tccccgttaa ctcgcgtaaa
    19441 ggcgcagata aaatttcagc gagtgaacgg aaaacccact ttggcgaaat ctattgcggg
    19501 ctggatccac aacaagtgac ttatgagagt gaccgctgtg tttattgcgc cgaaaaagct
    19561 aactgcaact ggcattgccc gctgcataac gctattccgg attacatccg tctggtgcag
    19621 gaaggaaaga ttattgaagc ggcagaactt tgccaccaga ccagttcctt accagaaatt
    19681 tgcggcagag tttgcccaca ggaccgtctt tgtgaaggcg catgtacttt gaaagatcac
    19741 tctggcgcag tcactatcgg taatctggaa cgctacatca ccgataccgc gctggcgatg
    19801 ggctggcgtc ccgatgtcag caaagttgtt ccccgtagcg aaaaagtggc ggtgattggc
    19861 gcagggcctg cagggttagg gtgtgctgat attctggcgc gcgcaggagt tcaggttgat
    19921 gtctttgatc gccatccaga aattggcggt atgctgactt ttggcatacc tcctttcaaa
    19981 ctcgataaaa cggtattaag ccagcggcga gagatattca ccgcaatggg aatcgacttt
    20041 catcttaact gtgaaattgg ccgcgatatt acctttagcg atttaacttc tgaatatgat
    20101 gcagttttca tcggcgtggg gacttacggg atgatgcgag cagatctgcc gcatgaagat
    20161 gcgcccggtg tcattcaggc tctaccgttc ctgactgccc atacccgcca gctcatgggg
    20221 ttgccggagt ctgaagagta tccgctgacg gatgtggaag gtaagcgagt cgtggtattg
    20281 ggcggtggcg atacgaccat ggattgtttg cggacctcca tccgcctcaa tgccgccagc
    20341 gtgacctgcg cgtatcgtcg tgatgaagtc agtatgccgg gctcgcgcaa agaggtggtc
    20401 aatgcgcgcg aggaaggtgt cgagtttcag ttcaatgttc agccgcaata tatcgcttgt
    20461 gacgaagatg ggcgtttaac tgcagtgggc ctgattcgta ccgccatggg ggagccgggg
    20521 ccggatggtc gccgtcgtcc tcgtccggta gcgggttcag agtttgaatt gcccgccgat
    20581 gttctcatta tggcctttgg tttccaggcg catgccatgc cgtggttgca gggcagcgga
    20641 attaaactcg ataaatgggg cctgattcaa accggtgacg tcgggtattt acctacccag
    20701 acgcatctga aaaaagtctt tgctggtggt gatgcagttc atggcgcgga tctggttgtc
    20761 actgcaatgg ccgcaggaag gcaggcggcg cgcgatatgt taactctgtt tgatacgaag
    20821 gcatcgtgat gaaatcgtta attatcgtta atccggctga ctgtattggc tgccgtactt
    20881 gtgaagtagc ctgtgtggtc gctcaccctt cagaacagga gttgaatgcc gatgtctttt
    20941 taccccggct gaaggtacag cgactggaca gcatcagtgc gccggtgatg tgccatcagt
    21001 gtgaaaacgc cccttgtgtt ggcgcttgcc ccgtgggggc gctgacgatg ggcgagcagg
    21061 tggtgcagac caattctgcc cgttgtattg gctgtcagag ttgcgtcagt gcgtgcccgt
    21121 ttggaatgat caccattcag tcgttgccgg gggatacccg gcaacaaatc gtgaaatgcg
    21181 atctctgtga acagcgggaa gaggggccag cctgcgttga atcttgccca acacaggcgc
    21241 tgcagttgct tactgaaaga gaactcaggc gagtccgcca gcagcgtatt gttgccagcg
    21301 gtaagaatcc tctctgaaag aaatctccgc gctgaaaggc gcggagaatc tattagattg
    21361 cccaaccacc cgcgtaaaat gcgaccagtg cggcagtaat gatcaccgta ccaatattca
    21421 gcttttgcca ttcgcgtgca aagacgcggc ctacgaccag tgtcacaaag cccagcataa
    21481 taccggtaac gatattacaa gtcagaacga tgaacacggc acacaccagg ccagccatag
    21541 cgtcaataaa atcattgaaa tccagcttcg agacgttact taacatcagc aaacctacgt
    21601 acatcagagc gggtgcagtg gcgtaaccag ggatcaaaaa tgacaacggt gataagaaca
    21661 gaatcagcag gaataacgcc cccactacgg ttgcggttaa ccctgtttta cccccggcgg
    21721 cggttcctgc cgctgattcg atataaaccg ctgcgggcgc tgcgcccacc aggccggaga
    21781 atattgaact tactgagtca ctggtcaggg ctttgccgcc gttgatgatc tggttgtctt
    21841 tatccaacaa attcgcctga ccggcgacgg cacggatggt gccagtggcg tcgaacactg
    21901 cggtcatcac caatgccagt acactcggaa gtacagttgg ctggagtgcg cccataatat
    21961 cgaggctgaa aatcagagac ttaccatctt cgccagtcag gcttggcatc gccaccagac
    22021 cgtggtattt caccgctgga tcaaagatta agccgatgat cgaaattgca ataatcacca
    22081 acaagatccc gccgggtacg cggcactttt ccagaccgaa gatgaccgcc agccccagca
    22141 agctcatcat cactgggaag gaggtaaacg caccgagcgc cactggcaag ccttcaatcg
    22201 ggtttttgat aaccattccc acaccgttag cagcaatcag cagcagaaac aggccgatac
    22261 cgatacctgt accgtgagct atccccatcg gtaaattacg taagatccag gtacgcacac
    22321 cggttacgga aatggcggtg aagatgacgc ccatcagaaa gaccgcgccc agtgcgacag
    22381 gaacgctaat ttgttgcccg agaaccagac tgaatgcggt aaacgccgtc aaggaaatcg
    22441 cgcaaccaat cgccattggc aaattggccc ataatcccat cagcaacgag ccgaagcccg
    22501 cgaccagaca ggtggcaaca aacacagctg cgggaggaaa acctgctttg cccagcattc
    22561 ccggaacgac gataacggaa taaaccatgg ccagaaaggt cgttaagcca gccagtactt
    22621 cctgacgaac ggtactgcca cgagcggtaa ttttaaaata attatccagc gcgccctgtg
    22681 gctttggtgc gtccggtgtt tgtaggatgt ctccagacat aatgatgtcc tcacgaggat
    22741 gttaaagttt tattattagt tgcgttcgta caccagacga ccatcaacgt aggtgcggta
    22801 gatcgaacgg tcatcgccca acgtcatcat cacgaacaat ttgtcgacta aagaaacaga
    22861 gttgtcatag cgcagctgct gtagcggagt ggcggtgggt tccatcacca cgaaatcagc
    22921 ctctttgcca ggtaaaaagt tgccaatcaa atcgtcaagg cccagagatt tcgctccgcc
    22981 gagcgtggcc aggtaaaatg cttcatatgc cgagaggcga tagccttgta gttgcaacac
    23041 tttgtaggct tcgtttagcg tttgcagcat gttgaaagtt gttccggcac cgatatccgt
    23101 tcccatgccc actttgactt tcttctgcca tgcttttttc aagttgaata agccgctgcc
    23161 gaggtaaagg ttggaggtcg gacagaaagc aatgctggat ttggtttcgc tgagacgatc
    23221 ccactctttt tcttcgagat ggacgcagtg agcaaagaca cagtttttac cggtcaggcc
    23281 gtactgatgg taaacatcca gatagccatc atgttcagga taaagttctt tcacccaggc
    23341 aatttcatct ttgttttcac agagatgggt atgtacccac gtatccggat attcttcttt
    23401 caggcgttgc gccatcgcca tctgttcagg agatgaggta ggggcgaagc gtggcgtaat
    23461 cgcatacaac agacgaccat ttttgtgcca gcgttcaatc agttctttgc tttggtgata
    23521 gctgctttcg gcagtgtcga gcagataatc cggtgcgttt cggtccatca tcactttacc
    23581 ggcaatcata cgcatattga tatgactggc ggcttcaaac agcgcatcaa cggattgcgg
    23641 atgaacagtg ccaaacacca gcgccgtggt ggttccgtta cgtaaaagct gcttgatgaa
    23701 gaacgccgac atctcgcggg cgtactctaa atcctcataa cgacgttcag tagggaaggt
    23761 gtgtttattc aaccactcca gcaattgttc accataggcc cccaccattt cactttgcgg
    23821 ataatggata tgtgtatcga caaagcccgg tactatcagt ttgccgcgat agtcgcgcac
    23881 gcgaatggtg tcaggaattt gatgttttcc gtcttcccat tcgccaaacc attccacttt
    23941 tccctgttta atgagtaata aaccatcctc aataaaccgc agcgcagagg caatctcttc
    24001 cgggttgtcg accgtacgcg tgacatcaat aaaactgcct cgtaccgctt ttaacgtgtg
    24061 ttctcctgac atcattgact cctttactgt taatccatct cttccgctga ggtaatacca
    24121 ggcagaacgt tttcctgtcg gtagccacca gggaggataa tatttaataa aatcgcagtt
    24181 aacccgccag cacaaatagg gttttcaact aatacataaa tagaggctgg caatatttta
    24241 aaaatttcgg gatcgtagga gacgccaagc cctaaaccta aagaagtggc gacaataagt
    24301 gtttcacggc gctttaaacc gttggtgatg atgatgcgaa tcccagcgat agcaatcatg
    24361 gaaaacatca acgtcattgc gcctcccaga actgccgagg gtatggttgt gaagaagccg
    24421 ccaatcatcg gaaacaagcc gaggataacc agcattaccg cgatggttcg cccgacataa
    24481 cgtgaagcga cgccagtcat ctgaataacc ccattatttg cgcaaacgtg gttaagggta
    24541 atgaaccgac agcggaggcg ataacagaaa ccagaccatc tgccagcacg ccgcctttca
    24601 gtcgggactg atactcttcc ccctgaatag ggcggcgaga aaccattgcc gtggcggtga
    24661 tatcgccgac agcttccagc acgctaagca aataaatcgt gccaaccacc aggaactgat
    24721 ggaagctgaa actaaacccg tatttgaatg gatgcgggat ggtgattaac ggcaaattgc
    24781 gcatactgct gaaatccacc atacccaggc ataacgacgc gatataaccg acgcatagcc
    24841 caatggcgat ccctcccatg cgtagcaacg gactgcgaca gcagttaaag ccgatcacca
    24901 caatcaaaac caataaacca acgccgagat gttcgtaatt gccgaacgta ccgctgcttt
    24961 tggctgcaaa tcctccacca aaatcgataa tgccgacttt aatcaggctt aagccgatca
    25021 tcagtaccac aataccgctg acggtaggcg taataacccg acgtaaatag ggcaagataa
    25081 atgaagatcc gacaaccaga aatgcgccaa cgaaggagac gccgagaagc gacgacatga
    25141 ttaactcttc gtgaaaaccg tcgcttttca tgctgctgcc cagcgcaatc atgaccgtaa
    25201 caaatgaaaa attgactgac tgaattgaga gtaggccaga accgacgatg ccgtagcggt
    25261 ttacttgtaa ccaggtacca ataccagagg cgatcatcgc catagaaaca agataggcgg
    25321 ttgtttcagc ggaaagctgt aaggccgcac ccacgattaa cgcgggggtt accatcggaa
    25381 cgaaaattgc caacagatgg gtaatggcac caacgagagc ctgatgaaag ggagggcgat
    25441 cctccagttc aaatataagg tcagaacctg catggtttat atcagacatc ccttcactcc
    25501 ttttaaagta aatttgatca tctgaatcag taaaataaat tcccacgaat ttggggcgca
    25561 ggacgccttg agaacggctt atctcatttt cgcagtcact atttagtcat cgtccaaaca
    25621 gagaaacagg ccgccgaagc ggccatttat tatttatatt ttttccagcg cggtgagtat
    25681 tttctccggt gtgaaatgcc attcgcgtaa ccagatgccg catgcatcgt gaattgcggt
    25741 agcaatcgcc ggagctgcgc catttacacc gatttccgag atcgatttcg ccccgaacgg
    25801 gccgacttta tcgtcgctcg gcaccagcac agcgcggaaa tcacgcggaa tatcgcctat
    25861 tttcggtgcg ccgtaactgc gtaaatcacg cgttaacggg tgaccttcgg cgtcataaat
    25921 gatttcttcg ctcatactgt ggccgattgc acgaagggtg gcaccgtaga tttgtcccaa
    25981 cgctaactct ggattgaccg gtgtaccgca atccagcaag gcgtagaatt tatccaggcg
    26041 gatttccccc gtacgcgtgt tgacggcaac ttcagcaaag tttgcgccat acgggaaggc
    26101 gaaatcaggc gtgatataac tgccagttcc caccagtgaa ccaaagccgg tgccggtttc
    26161 gcctttatgg gcaatatccc cgaaactaac ttcgcctttc ttgccgcgta cgacgcccgg
    26221 cgttgctagt tggacatctg ccactggctc acccaacatt tgcgcaccgt ggaacagaat
    26281 tttctcccgc agattttctg ctgccaagcg cgccgcgtta cccgagaagc aagtaccgga
    26341 cgaggcatat gcgcctttat caaacaacgc atgatcggta tcaccggaga taacatgcac
    26401 gtcctgcggt gggcagtgca gcacttctgc tgccagtttc gtcaccaccg tatccagacc
    26461 agtaccaata tccgcaccgc cagaatgaac gataaaggta ccgtctgatt ccagtttgat
    26521 catgcagtta gcctgatcga tatctgggat ccctgatttc tgcatgataa tggcgacgcc
    26581 acgaccgatg tgccagtcac cgttttgtgg ttttggtgaa gaccattgga tcatctcgcg
    26641 accctgacgc aggatctctt ccagtgcgca gctggcggcg gaaggaacgg aggtcggcgc
    26701 tttaccttca ccgattgcac cgagaatttt cagctcttgc ccttcgtgta cccggttacg
    26761 ttcgataatt tccagttggt cgatctgtaa ctgttcagcc agttccgcta atgccatggt
    26821 gatagcgaag ttacctttcg gtgcgccata accctgataa gcaccatttg ggcaaatgtt
    26881 gctgtagtag gtggtgacct ggaaatcgac gttatcgcac ggatataacg gcagcgacag
    26941 cgccggtccg ttacacggta cggtgagtga gtggttgccg taagggccag tgttggcgcg
    27001 gaaatccatc ttcactgccg tcaggcgacc atcttttttc gctcccagtt tgacggtgac
    27061 tttcgcgacg tgacgagagg tgttagcaat aaactcttct tcacgggtgt agcggaacag
    27121 taccggacgc ccggtcacgc aggttgccca ggcgcacact tcttccagca ggatgtcctg
    27181 tttagaccca aaaccgccgc caactcgctc tttaatgaca tgaactttat gttgtttcat
    27241 gcccacgagg cgcgcgacct ggcggcgtaa gtgccatggt acttgggtgg aggcgtggat
    27301 aaccagacga tcgccgtcca tacgggtaaa gcagatatga gtttcagtcg ggcactgctg
    27361 cgcctgcgtt gagttatagg ttcgctcaat gatcacatcg gcatcggcaa agcctttgtc
    27421 catatcgcca atatgaccat gaatactggc ggcgatattt ttacgggggc gagaaccgat
    27481 cgggaaattg atgatcatat gctcgccgcg ctgggctgca tggctgttat cgtcttccag
    27541 agtatctggc gcaccagcaa catacaccac cggttcatcg tgcacgacag gcgcatcttc
    27601 cgccattgct tcgtcgatcg acattacagg tttaagcact tcatattcaa cgtcgatgag
    27661 cttcagtgct tcgagcgcaa tttcttcact ttctgcgacg accgcagcaa cgcgatcgcc
    27721 gacgtgacgc attttcttgc cgaacatacg gcggtcaagc ggtgacggtt ccggtgcgct
    27781 ctgaccgccc ggggtatagt agatatccgg gcaattcagg tgagtaataa cgtgaacaac
    27841 gcccggtaag gcttcagctt tgctgacatc cagatgagta atcagtgcgt gagcgtgtgg
    27901 gctacgtaac attttaatga cgcaggcgtc agccgttacg cggtcttcaa catagcaggg
    27961 tttcgcctgc accattttcg cggcatcagt tttaggataa tgcttgccaa tgacttcaag
    28021 gtcgtcacgg aaagtcggag cgatatcaat ggtggcctgc ggattatttt tacgtgcaac
    28081 cgccagttca atgacctgat agtattgctg ccagccagca tcgcggctaa acagacctga
    28141 gagtgcatcg tcgatctctt cgcgggtagg tgcgtcgatg cgatcgagaa gatcggtgat
    28201 aatcagagct gcagctggat cgttataacc agactgcacc acgccaacat caaccatggc
    28261 ctgttgaacc agacttaact cattccattt gcccagagat tctgcagtac gaatatctgc
    28321 cttctctaac tgtgcggcga taagcaagga cgcgttaacg atattaccgt taaagataat
    28381 tgcgtcagaa ccggcaaacc caaagccatc atcactgttg cgtacagagt gcattcccat
    28441 gttaaacaac agcttttgca cgttttcgcc tggattaacg gttagctcct gaggcgcgcc
    28501 atttaaagta aagtggatga tcatacggct tcctccccag cctgttggca gtcggcatac
    28561 aggtcggcta ccagaactcc cgtgatatag cgtttatagg tcacgctgcc ccgcaaatct
    28621 tcctgcggga aaatggcgtt ggcgacagcc tgttcaagtg cattgccttc cagattttgt
    28681 ttttcgacat cgtgcagacg cagggcttta ctggcgacgc catccagcgc aattcgcata
    28741 ccgtcgtggt ctgtcattgc aacggctgcc gtcacgacgg ttaaacctgc ctgagaacgg
    28801 ctaattttgc gggtcgcaca ggtgcgatac ggatctttaa tgataatttc ggttagcagg
    28861 cgatcgcatg ggcaggccag gtagtcctcg attgacagcg tttcgccgtt gccaaaaacc
    28921 agttcagcat ccagtgccag caggacggga agcagcaccg actcttcctg ccgggcggcg
    28981 atttcaccac caatggtcga ctgattacga acatggcgtg agtaaacaaa accgagggct
    29041 tcacgcagtg ctgcaggaat aaatcgcgca tcacgcagtg gctgcaagcg agacattgca
    29101 ccaatccgca gtgcaccgtt atcccagtca acccagtcca gttccagatc ctgtaaggaa
    29161 atggcaatct ttttatcggt acgggttggt gtagcgttga gtttgctgcc cccggcgaac
    29221 cagacggctt catccttgta gcggcgcttc agttccagcg cctgttcgac agagtcgggc
    29281 ctgaaaaatt gttcaatcat cttctttcct tatttaaccc tcttccctgc gggaagaggg
    29341 tatttgtcat ttatgccagc gcatccatcc gacgccacat actggcagcg gcttttctgg
    29401 cttgcgcata aatggaatcg caatcgaagt taaactgacg gtcttcatag accatcacgc
    29461 cattgaccat cacgctatga acgctgcctg agcccatacc gaaagcgata tgcccggcga
    29521 tattgtctgc aagcagcggt gtcggcgagt tgtaatcgca aatggttaaa tcggctttgt
    29581 aaccggcttc cagaaggcca aatttcgcgc caaagttgcg gctcatcagt tcgttgccgt
    29641 tagccagtgc tttggcaaaa ctgtcaggcc acagcggacc acccgcatcg cgatgtttaa
    29701 agaaggcaaa tttcatctct tcaaacatgt ccgaaccaat gccgtcagtt cccaacgcca
    29761 gattgcggat gtcgctaagg tgatggttgt agccgacatg gttgttcatg tttgaacggg
    29821 cgttatgcac caggaacgca tcgcgctgat tgagtagggc gatgtcatct ttcgacaagt
    29881 acagcccatg agcgaccagc gttttgctgt cgatgagatc gaattgcgcc agtcgtgcca
    29941 gcaggtcttt gccgtaccag tggtgactgt aggaaacgtc gtaaaggtct tccgcagcgt
    30001 gaatatgcaa accacgatct gtggctttca cggcttcacg cagcatctcc agaccggcat
    30061 ccggcacggt aaacggcgcg tgagcgccga tatgtgcttc caccagatac ggctcggttg
    30121 ctgctttctt cgcctcatca atctgacggg cgaagcggat gttttcttct acaccttcct
    30181 gcaactcttt gatgccgttg ttacggtcag tagtttcaaa acaggtcatc gcgcgcaggc
    30241 caacttttaa aaatgcgttg cgcaatgtgg agagcgaccc gccgatatac gccggagagg
    30301 catggtgatc gataaccgat gtacatccgc tcttaatcgc ttccagggaa caaatcagtc
    30361 cgctgtaata gagcgactct tcatcaaggg cgcgatcgag tcgccaccag agatttttca
    30421 gcgttgagat gaaatccggg cagggggcga tgtttgccat aattccgcgg gacagtcccg
    30481 agtaaaaatg gttgtgcgag cagacaattc ccggcatcac aatccgacca tgcatctctt
    30541 tgtagctggc atcggggtag cgttgcgtca gggcatcgcc gatagcgaca atcacatcat
    30601 tttcgatggc gatatcaacg ccttcctgca ctttcgccgg gtgtaactgc actgcggtga
    30661 cattcttcag aatcaacatg attacacctc cacgcggccc agcagataat gatgatgctg
    30721 gtggacatgg ctaataatgc ggcacatatc gttcagctcc ggtggtacgt tgttgaactg
    30781 accttcgctg tcgatgttta acacccagct ttggttattc agacgtactc gtacccggca
    30841 atcttccaca aggaagccag gattgctgct gttatcaaag tcttgcgaca ggctgaagac
    30901 ggtgattttg tctttgtacg gtttaccgtt ccacgggcag aactgagcgc agttgccgca
    30961 ttcgttacag taagcgtcga ggtgcagcgt ctggaaacgg ttctggaagc ctgggaccgc
    31021 aatggatacg ttggcgcggt tcgggcagac atccacacac ttgctgcaaa cgtagttaca
    31081 ttcaaggcag cgtgcggctt cctgggcgac aaacgcgtca cgatcgtcgc tatccaccag
    31141 ggtgatagag atatcgcctt tgcgttgata gatttccgcc ggattgacgt tgttccagta
    31201 tttatcgttc tggtgggaac ggatgttttc ccgactaagg atgacatcgg tcgcccgacg
    31261 cgcggttccg acagcagcga caatggagga tggtccgcgc tgtacgtcgc cgatcataaa
    31321 gacgtcagtc agacgagttt cgccattatg gtcgacgtct ggccagccgt ttttgtccag
    31381 cggcacgccc atcgcattca gggcttcagt atcctgctgt tcaccaatgg cggtgatcag
    31441 gctgtctaca tgcagtgtta ctgtttcgtt ggtttcaacc ggacggcgac gacctttctc
    31501 atcaggttcg ccaagcgaca taacgcgcaa ggttaaggtg ccatcagcat cgaaacgttc
    31561 cggattattc aggaaacgga actccacgcc gtcgtgcaac gcttcttcat actcttcgcg
    31621 ccatgctggc atctcttgca gtgaacgacg gtaaacgacc gttgcttttt ctacgcctgg
    31681 aacgcgtaac gccgcacgag cgcagtccat tgccgtgtta cccgccccga caacgaccac
    31741 atgtttgccc agcttgagcg ctgtgccctt gttgtattca cgcaggaagg ggagtgattt
    31801 ccagacattt tggttgtcgc ccgccagttt cacaccgcta tttttatcag tgccggtggc
    31861 aatcagaaca tagtggaagc cctgattttt taactgttca acggtcaaat cgggtgagca
    31921 gccatactca aatttcacgc cgtgagcggc aacaaaatcg atatcgtgct gaattaactc
    31981 tgcaggaata cggaactgag gaatgatatt tttcaccacg ccgcccgcat tggcttcgcg
    32041 ttcaaacagc gtaaccggat ggcccgctct ggcaaggaag taacctgctg ccagacccgc
    32101 cggacctgca ccaatcacgg caaccggatg gcgtgaacca gaaccggctg gtttgtgcca
    32161 gcgttgctta tattcatccc aacctttttc cagcgcgact tttttcagtt cgcggatatt
    32221 cagcgcactg tcgtaatcca ggcgggtaca gttgtattgg cactggtgat cgcaaatatg
    32281 accggtaatg gcgggcaggg cgttgcgttg gtagatgagt tccagtgcgt cggcatagcg
    32341 gtgttcgcca agcagacgga tgtattccgg aatatcctgt ttaatggcgc aagcagtaac
    32401 gcacggtgca acgtagcagt cggtcagcgg caggtcttct gccacttcaa tacgctcttc
    32461 tggcttccag tgtttctgag tgtattccat ggttaacgcg tctgctgcca gtttgttcag
    32521 gcgttcgacg tcaacatggt caagccccca ggcgtcggag ccttccagct cgcgcatgca
    32581 ggcacttaag cgcagatagc cgccaggttt cagcaggtcg gttgccatag taatagggcg
    32641 aataccagta tcaaaaatat cgcggatagt cagctgactg gcaccaccgg aataagaaat
    32701 gggcagtttg ccgtcaaagg cgcgagagag aactgctgca acattgatgg agagcgggaa
    32761 cagcgcacgg cctgacatat acatctcttc accaggcagt gcgcctttat tgttgatggt
    32821 gccgagagtg ttagtcagtt ttacgccaaa gccgagtgat ttttcttttg ccagtaccat
    32881 caggcgttcc agcatttcca gcgcttgcgt cagcttgagg tcgtgatcaa atgactcttc
    32941 ttttaagcct atgtaaccga aaccgcagac atcgaggatc tcacgaacac gcgcgtaccc
    33001 cagtaaggtc gggttgagtt tcacaaaggt attgagccct ttttcttcca gcatgtagcg
    33061 gcaaatggct tcgatttcat gcggaggaca gccgtgcatg gtggagaggg taacgccttg
    33121 taccatgctg gtggggatgc gtgccggtaa ggcctgcagg cattcgcgtt tttcctgcaa
    33181 tccgtggcgg gctaaaaatg cgtcatcctg gagtaattta ttcagcgtat cgcgatattg
    33241 agcgaatttc ggatggtcag atgcatccat catattgtcg atgaactgtt gcatcggcgg
    33301 ttgcttaata ccttcgaggt tataaccgac gctcatatta aagatgaacg atttaccgga
    33361 atcagaaggc tggaacatcg cttcgagaag gtgcagggca aaccaggctt tgaggtattc
    33421 atcccaggct ttaagcaggg taaattcggt agaccattcg gtgttaaagc actcgtcttc
    33481 ggcatcgata cagggctttt ccagctccag acggtcaaga atttggacgg tttttagttc
    33541 gatgaagcgc ccgccagtca gccaggaagt aacaatattt tgcgcgagct gcgtgtgcgg
    33601 gccagcggca gggccgacgg gagtggcaca ggtttcaccg aagacgctaa cagttttacc
    33661 ttttacgggt gagtaaaact gttgctcggg aataccaaag attgagcgtt gttgttggta
    33721 ttcatcaaat atgcgcgtca aaagttcctc aaacggaatg ggacgcataa tatcccccat
    33781 aaccctctcc ttagcttata cagatatcat tgagtgaccg gtgttgagaa atccggtggt
    33841 ttagggggat agagcaacaa tcgcaccact cttgccaggt ttggcgggaa atataaaaaa
    33901 atgtgaggcg gctcgaaact taggagcttt ttgtgaatga actcacaggt gccaaatgag
    33961 aattaaaatc actcctgaaa attattattg ccagcccatt tatcactttt tctcttttct
    34021 caaaatcagt gattccttat cattatgata aagagtgctg gagggattaa atatttgaac
    34081 gcaatcgttt aactacattt tctaaatttt agtatacctt ttttgcgctg taataaagtc
    34141 gtccggagta tcaatatcta aaattatttc tgcattttct atttcgacgg aataatgatc
    34201 tcccataagg agtgcctgac gcatattggt gatattggga cgctggattg cctgcatcag
    34261 gcatggcttt gacactaaaa tcggatggcc gggaatgcca ttatggagtg gcagtattgc
    34321 gccatcattt cgtaacgacc agatttttct aaaaatatcg atggtgaggg taggcatgtc
    34381 accgtgggtg agaaaacaat attctgtttt taccgcgggt actgcggcct ttactgatgt
    34441 gagtaaaccc tgcgcataat ctgggttgtg aataatagtg atattgctct ggttcgcata
    34501 acgttcgtgc agttcattac cacgatagcc ggtgactaaa ataattcggc tacaaaactg
    34561 caacgcattt ttgatacttg tatcaagaat tgttccctgt tgccagggta acatcatttt
    34621 ccattgcccc attcttgatg ataatccggc agcggtaatt atacagtcga tggctgacat
    34681 agtggttcca ttatttatag gggctaataa cgcctgataa ttgcgttact tcctgatgga
    34741 ttaagttggg gataatttat cccagagagg tcataaagac tcgtgaaaag tatagttgac
    34801 ccatcggcat tattcattga tttaggcgcg cagaaacgtc ccactgtgat ttctgttgtt
    34861 ggggctgggg ggaaaaccag cttgcttttt tggctggcag aactgctcca ggctagcggc
    34921 aggcgtgtgt taatcactac gacgacacat atgtttatgc caacgtctca ctggcccgtg
    34981 gttttctgtc gtgatcccgc catgcttcct catgcgtctc ttacatcccc catttcattt
    35041 tgttttcaca gctggaaagc gaaccaggga aaggtgcagg gatttacgcc agaagcgatt
    35101 gatgcactgg tgcaacgacc agagtgtgac gtaattctca ttgaggcaga tggctcgcgt
    35161 ggaatgccgt taaaagcgcc tgatgagcac gaaccttgca tacctaaaag cagttgctgc
    35221 gtgattgctg tgatgggagg acatattttg ggtgcaaaag tgagcacaga aaatgtccat
    35281 cgctggtcgc agtttgctga tattactggg ttaacacctg atgcaccctt gcaactgagc
    35341 gatctcgttg cgctggttcg ccaccctcag ggcgcgttta aaaacgtacc gcaaggttgt
    35401 cggcgagtct ggttcattaa ccgtttttct caatgtgaga atgcgattgc gcaaagcgag
    35461 ctccttcaac cgctgcaaca acacaacgta gaggcaatct ggctgggcga tatacaagaa
    35521 catcctgcaa tcgcgcgcag atttgtgaat tagcggcccg ggtattccgg gttactgaaa
    35581 ggatatccat tgaggttggt atgaatattt tcacagaggc tgcaaaactc gaagagcaaa
    35641 attgtccgtt tgcgatggcg caaattgtag atagccgagg ctctactccc cgccattctg
    35701 cacaaatgtt agtgcgcgcc gatggttcta tcgtcggtac aattggtgga ggaatggttg
    35761 agcggaaggt gattgaagag tcgcttcagg cattgcagga acgtaagccg cgattattcc
    35821 atggacgtat ggctcgtaac ggtgcggatg ctgtcgggtc agattttgga ggtgcaatgt
    35881 cagtatttat cagcgtccat ggtatgcgtc cacgtctggt gttgattggc gcggggcatg
    35941 tcaaccgggc gatagcccag agtgcggcgc tattaggatt tgatattgcc gttgccgata
    36001 tttatcgcga aagccttaat cctgaattat tcccaccatc aaccacgctt ctccatgctg
    36061 agtcgtttgg tgcggcagtg gaagcactgg atattcgccc tgataatttt gtcctgattg
    36121 ccacgaataa tcaggatcgt gaagccctcg acaaactcat tgaacagccc attgcatggt
    36181 tggggttgct ggcaagtcgt cgcaaggttc agcttttcct gcgtcaattg cgtgagaaag
    36241 gcgtggctga agaacatatt gcccgtttac atgcgcctgt tggttacaac ataggtgcgg
    36301 aaacgccgca ggagatcgcc atcagcgtgc tggcagaaat attacaggtg aaaaataacg
    36361 cgccgggtgg gctgatgatg aaaccttctc atccttccgg acaccagctg gtggtgattc
    36421 gcggtgcggg ggatatcgcc agtggtgtgg cgctacgtct gtatcatgcg ggttttaaag
    36481 tgatcatgct ggaagtggaa aaaccgacag tgattcgttg taccgtggcg tttgcccagg
    36541 ccgtgttcga tggcgaaatg acggtcgaag gcgtcactgc ccgcctggta accagctctg
    36601 cggaagcgat gaaacttacc gaacgcggat tcatccctgt gatggtagat cccgcctgtt
    36661 cattgcttga tgaactgaaa ccgctttgcg tggtggacgc tattctggcg aaacagaatt
    36721 tggggacgcg ggcagatatg gcaccagtaa caatcgcgct tgggccgggc tttattgcag
    36781 ggaaggattg tcatgcggta attgaaacaa atcgcgggca ctggctcggt caggtgattt
    36841 actctggttg tgcgcaggag aataccggtg ttcctggcaa tattatgggg cataccaccc
    36901 gacgggtgat ccgtgctcct gctgcaggca ttatgcgatc caacgtgaaa ttaggcgatc
    36961 tggtgaaaga gggcgatgtg attgcctgga ttggtgagca tgaaattaaa gcaccgttga
    37021 cggggatggt gcgtggcttg ttgaacgacg gactagcagt ggtcggtggt tttaaaattg
    37081 gtgatatcga tcctcgtggt gaaacggctg atttcaccag cgtttctgat aaagcccggg
    37141 cgattggcgg cggcgtactt gaggcgttaa tgatgttgat gcatcagggt gttaaagcga
    37201 cgaaagaagt gctggaagtg gcttaaatga aaatggccgg atggtattac ccacccggcc
    37261 tttaaattac tgacaaatca ccgttccggt tttgccttca atcccttctt tcgctttgct
    37321 caatacggta atcagtgctt cacgacctgc gcgagagcga gcaaacgagg cggcagcttc
    37381 cactttaggc agcatagaac ctttcgcaaa atgaccttct tcgataaagc gttcagcatc
    37441 acttaatgac aggcgatcga gccactgttc attctcttta ccgaagttaa tggccacttt
    37501 ttctaccgca gtcagaatga tcagcatatc ggcatcaatc atttctgcta aacgggcgct
    37561 ggcccagtct ttatcgataa ccgcactggc accacgcagg tggttacctt cacgaataac
    37621 agggatacca ccaccgccaa cggtgatcac cacctggcct gcatctacca acgctttaac
    37681 cgtttctttt tcaatgatat caaccggttt tggcgaggca acaacacggc gatagccgcg
    37741 accagcatct tctttcagag tgtaaccctg ttttgtcagt tgctctgctt cttgctcggt
    37801 aaagaacgaa ccgatcggct tggtcgggtt gaggaatgcc ggatcgttag cgtcgacttc
    37861 gacctgggta accagcgttg ctacaggttt attaatgcca cgagaaagca gttcttctct
    37921 taaggcattt tgtagatcgt aaccaatata accctggctt aaggcaacac agacagacat
    37981 cggcagcatt ggcgagtgcg cttcagtttt tgcggcagct tcaaaggcct ggttaatcat
    38041 gcccacctgc ggcccgttac catgagtgac gatgacctca tgtccctggg caattaaatc
    38101 aacaattgcc tgagaagtaa tttttaccgc tttcatttgt ccggccaggt catcgcccag
    38161 cgcattcccg cccagggcga gaacaatttt cttactcatt tttcctcact catcattctt
    38221 gattgtgtat agatccctgg cagtggttag agcacgggag ggacaaacgg tttgcgtcgc
    38281 aggaatcgac ctcgcccggc tttgcctgta aaggtgccgt cacagaaaat cgtttcacca
    38341 cgggataagg ttctgacaat cgcgccctga caggtaaaac cctcccatgg cgagtagtcg
    38401 gcgttgtcgt ggagatggcg atgctgaatt tgttggctct gacgtgggtc gataatcacc
    38461 acgtcgccat cggaaccggg cgctaataat cctttttgcg gccacaggcc aaacagcctg
    38521 gcgggcattg cgctggttaa ttcaacaaag cgttccggtg ttatacgtcc cgtcatcacg
    38581 ccactggaaa acagtaactg catgcggttt tccacaccgg gtaagccatt tgggcagcga
    38641 ctgaaatcgc ctttagaaat ttgcaggcgt tgagccatcg agaaggtgca gtgatcggtt
    38701 gccaccacgt caatcgcacc atcgctgatg ccacaccaca gtttgtcctg ctcgcgtatg
    38761 ttacgcagcg gtgggctaag aatgaacttc atgccatctt ctgtatcgta actgcgttcg
    38821 tccaacagga gatattgtgg gcaggtttca acccagactg gctggtgatt cgcacgggca
    38881 agacgcagat aatccagacc taagccgtta gacaggtgga cgatatagag cggggcgtta
    38941 ccggcaattt gtgccaggtt aatcatgcgg gcgatggctt ccgcttcgca ttccagaggg
    39001 cgactcaagg cgtgatagcg cggcgcggtt aaccctgcgg cgataaattc cgcccgcttg
    39061 ctggcgatag ccgcatcatt ttccgggtgc acggtggtca gcgcgccgga ctcatgcaag
    39121 cggcgtaacg cctgcaaaac ctcgtcatcg ttgagtttgt attgataggt taaatagagt
    39181 ttaaaactgc tcagtccttc ttcgaccatc atcggaattt cgtcgaggat ggcgtgatta
    39241 atgtgctgga tcacaccgtg aaagctgtag tcgatgaccg ccttatgggc ggcatagcca
    39301 cgataaactt ccagttgatg gcgtaatcgg cagccattcg ggccaaatcc catatggtca
    39361 ataatggttg ttgtaccgcc acacgcagct gcgcgggtac cggtaaaaaa atcatcacaa
    39421 ctgcgcgcga tgccgacatc aatattgaaa tgcgtatgga catccacgcc gccagggaaa
    39481 acgtaacagc cagtggcatc aatttcttca tacgggagct gcggcgaaat attgctgccc
    39541 aattggcgaa caatcccgct ctcaatcagc aaatcttgtt tggcttgtcc atctgcgtta
    39601 acgacagtgc cgtttttgat caatacgcgc atagcaaact ccaaaaaccc tccggcgaac
    39661 cggaggggaa taagtgaaga gtaattattc ggttgccagc cagcttaacg ggattgcagc
    39721 gtacatcgcg gcacaggtca ccaggtgaga tttccaggtt ttttcgttag gtgcatgcgc
    39781 ttcaggttct ttacccgggc caaagccgat caccggaatg ccgtgacggc ccatgataga
    39841 tacgccgtta gttgagaagg tccacttatc aacaaccggc gctttgccaa acagaccttc
    39901 gtaggcattc accagtgctt tgacagtgaa gtggtcttct tccactttcc aggtcgggaa
    39961 gtagcattcg gttgggtaaa ccaggccagt ccaggacgga cggtcgtagt tgtacataga
    40021 aacaaccgcg ttagctttct gtaccgcagg cagggcgcgg atttcgtcca gcgcgccttc
    40081 ccaggtttcg ccccaggtca gacgacggtc gattgaaact gcgcagctat ctgctacagc
    40141 gcaacggctt ggggatgtga agaagatttc agaaacggtg agggtgcctt tgccgaggaa
    40201 ttcatcatag cccaggcgct gggagagttc ttgtaattcg ccaagaatcg gacccatttt
    40261 gaaaatggcg ttgtcaccgc gttctggtgc agaaccgtgg cagctaacac cttgaacatc
    40321 aatgcgaatt tccatacgac cgcgttgacc acggtatacc tggcaatcag tcggttcagt
    40381 actgaccaca aattccgggc gaatgccgga ttgttcaata atgtactgcc agcacagacc
    40441 gtcgcagtct tcttcctgca cagtaccggt aaccagcagg gtatattcat cttccagacc
    40501 gaggtcttta atgattttac cggcataaac catagatgcc atgccgcctt cctggtcgga
    40561 agtaccgcga ccaccgatca gctcatcagt ttccatgcct tcgtacggat cgaagtccca
    40621 gtttttgatg ttgccaatgc cgacggtatc gatatgagcg tccattgcca ccagacgcgg
    40681 gccgtggccg atataaccga gaacgttgcc catcgggtcg atttcaactt tatcgaagcc
    40741 gactttttcc atctcttctt taatacgatg tactacgcgt ttttcgtcgc agctttcact
    40801 gggaatagca accatgtcgc gcaggaagcg agtcatgtcc gcctggtaat cttttgcttt
    40861 ttcaagaatc agtttgaatg gaatattctt agccattatt tttctccaga ttttttactc
    40921 cccgttttgc agggagcagt tccaataggg tgattaaggt gctacagcgt gtttgccttc
    40981 ccagacaact tcgcggtagt gcttcacgtc tgtgtcgcct tcagtgctga taaccagcac
    41041 tacggcatct ttgttcagcg ccagtttttc catcaggctt tgacgttgcg ggtgataatg
    41101 aaccgctgcg agaacgccca aaccgacagc gccggattca ccggagatga tgcgtgggtc
    41161 attgccgtac gggttaccca gcacacgcat acctaatgcg gcaacgctgt cctggcagga
    41221 gatgaattgg gtggcgcagt tacgtaggat ttcccagccc agcgggttag gttcgccgca
    41281 ggccaggccc gccatgatgg tggccatatc accgccaacg ttgacgatgt cgcctttgac
    41341 gccggagcga taaatacagt cagctttgtc aggttcaaca ataatgctgt gcagattttg
    41401 cgggctgtag acgtcgacca gataacccag cacaccaccg gccattgcac cgacaccggc
    41461 ttgcagcaga acgtgcgtcg gggttacgcc catttcacgc atttgctcga cggcttcatc
    41521 tgccagtgtt gcgtagcctt gcatgatcca ggttgggatt ttggtgtaac cttcccaggc
    41581 ggtatcttgc accacttccc agccgtgctg ctgcgcgtgt tgcatggtca ggcgaacggt
    41641 atcgtcatag ttcatatccg tgacgatgca ctcggcaccg aggttcagaa tggcgtcaac
    41701 gcgttcctga gcagaacctt tcggcatgta aatcaccgca ttctgtccga gttgctgtgc
    41761 tgcccacgcc acaccgcgcc cgtggttgcc gtcggtggtg gtcgcgaaag tcattttttc
    41821 gccgatggca tttttcaggt gctcaaatga cagcgtttcg atatcaagat gatatttttc
    41881 gcacaataat tgagcgatgg cgtatgcacc gccgagcatt ttgaaggcgt tcagaccgaa
    41941 tcgttttgat tcgtctttga cgagaatttt cttcacacca aaaaggtttg cgagatcgtt
    42001 cagcgcacaa agcggtgttg ggcgataacc agcaattttc tggtggaact ggcgcgccag
    42061 tttggcctgg ctttgcgaaa agagcggtga tgtttcgccg ttgaaaaatt tgttatcggc
    42121 gatatcaatc ttcaatgaga aaacggacat atcctatcct tttggcctct ggaaatagag
    42181 ggccgaccag ctgatcggcc cgaggggtta tttcacgcgt tcttgcgctt ctttcagcag
    42241 ttgctcgagc agtgcacctg gtttggcgta tttacgggac aggatcatcg cggcgatgat
    42301 ataaggcttc cagctggctt ctttgtaggt cgcgatacgg tatttttcga atacgccttc
    42361 agtcacttca ccttctttgc aggatacgcc gctgatatca gctggcaggc agtgcatgta
    42421 cagggcttcg ccatcacggg tcagttccat catctcttca gtacaatgcc agtctttgtg
    42481 ttgcgcgttc tgtgccagac actgtttttc cagtgctttt aagccttcgt gatcgttcgc
    42541 acgcagcaat tcagtacgct cttccatcac tttgtaaggt gcccatgact tcggataaac
    42601 gatgtctgcg tctttgaagg cttcttccat gctggtgacc tgacggaagc taccgccgga
    42661 ggctttagca ttgtttttcg ccacttcaac gacatccggg atcaggtcgt agccttccgg
    42721 atgggccagg gtgacatcca taccgaagcg agtcatcaga ccgatgatgc cttgtggtac
    42781 agagagcggt ttgccatagc ttggggagta ggcccaggtc atggcgattt ttttaccttt
    42841 caggttttcc agtgaaccaa agtgttcacg taaccatgcc aggtcagcca ttgactgagt
    42901 cgggtggtca atatcgcatt gcaggttcac taaagccgga cgctgcggca gtacgccctg
    42961 cttgtagccg tcatcaagtg cagcgccaac ttcacgcata taagcgttgc ctgcgcccag
    43021 atacatatcg tcgcgaatac caatagcgtc ggcgcagaag gagatcatat tggcggtttc
    43081 acgcacggtt tcgccgtgag cgatttgtga tttgccttca tcgagatctt gctgtgcaag
    43141 gccgagcagg ttaagcgcgg aagcataaga gaagcgggta cgagtggagt tgtcgcggaa
    43201 tacggaaata cctaatccac tattaaagac tttggttgag atgttttcag cacgcagtgc
    43261 ttttaatgct gcggcaacgt ccagtacttg tttcagttca tctggcgtct gttcccacgt
    43321 taacaaaaaa tctttctcgt gaaggtgaga ggtcagcgaa ttgatatcct taatcagctc
    43381 attaacagtt ttcatcataa agtcccggta gtggaatgac aggcaaagtg gtttatttca
    43441 ctgtgacttg ccaaagtgat tggtgttttc acagctatac ctgggactaa caataagtgt
    43501 gccagaatta tccacggggc tgcaaaagac taatttgacg gataatgtgt ggggttgatc
    43561 acaaattgat accagcttct ggttgtttgt aaaatacgaa aacgtttggc tttttttatc
    43621 gcgttattta tttgctgcta agtatcagaa ataagataaa ggggcaggtt atccctcgtc
    43681 agtttgataa actcaccgcc gtaattctca tattgatata gaaatgtgat atctcttcca
    43741 taatttcatt agaaagtgat aaaaatctta tgtttttatc aaagccaatt tcgtctgagt
    43801 taatatcatc atgatctgtg aaccatcaac gtcttcaatg atcgactttt aagtaagtct
    43861 ggaaggtaaa gctatggagc ttgctactac gcagtcagta ctgatgcaaa ttcaaccgac
    43921 aattcagcgt tttgccagaa tgcttgccag cgttttgcag cttgaggttg agatcgttga
    43981 tgaaaacttg tgtcgcgtcg ccggaacggg cgcgtatggg aagtttcttg gccgccagtt
    44041 gagcggcaac tcacgcctgc tccgccacgt cctggaaacg aaaactgaaa aagttgtgac
    44101 acaatctcgc ttcgatcccc tttgcgaagg ttgcgatagt aaagaaaatt gccgtgaaaa
    44161 agcatttctg ggtacgcctg tcattttaca ggatcgttgt gttggggtga taagtttgat
    44221 tgccgttacc cacgagcaac aagaacatat cagtgataat ttacgcgaat tttctgatta
    44281 cgttcgccat atatccacca tttttgtttc gaaacttctg gaggatcagg ggccaggaga
    44341 taacatcagt aaaatattcg cgaccatgat cgataatatg gatcagggcg tattagttgt
    44401 tgatgatgaa agtcgggttc agtttgttaa tcagactgcc ttaaaaacac ttggtgttgt
    44461 acaaaataat attattggga aacctatccg tttcagacca ttaacatttg agagtaattt
    44521 tacccatgga catatgcagc atattgtttc gtgggacgat aaaagtgaat taatcattgg
    44581 tcaattgcat aacattcagg gccgacaatt atttttaatg gcgtttcacc aatcgcatac
    44641 cagtttttct gtagcaaatg cacctgatga accacatatt gaacaattgg ttggcgagtg
    44701 ccgtgttatg cggcaattaa aacgactcat tagccgtatt gcacccagcc catccagcgt
    44761 tatggtggtt ggtgaaagcg gcacgggtaa agaagttgtc gcccgtgcaa tccataagtt
    44821 gagcggaaga cggaataaac cctttattgc tatcaactgt gccgcgattc cggagcagct
    44881 tctggagagc gaactgttcg gttatgttaa aggcgcattt actggcgctt ctgccaacgg
    44941 taaaacaggg ttgattcagg cggcgaatac gggcacgctg tttctcgatg aaataggtga
    45001 tatgccatta atgttgcagg ctaaattact gcgcgctatt gaggcgcgtg aaattctgcc
    45061 gattggtgcc agtagcccaa tacaagtcga catccgcatc atttctgcaa ctaatcagaa
    45121 tttggcccag ttcattgccg aaggtaaatt ccgcgaagat ctcttctacc gacttaatgt
    45181 tatcccgata actcttccac cgctgcgtga acgccaggaa gatattgaac tactggtgca
    45241 ttacttttta catctgcata cccgtcgtct gggatcggtt tatcctggca ttgctcctga
    45301 tgtcgtcgaa atattgcgta agcatcgttg gcccggaaac ctgcgcgagt taagcaattt
    45361 gatggaatat ctggttaacg ttgttccttc aggtgaagtt atcgatagca cgctattgcc
    45421 gccaaatctg ctgaataatg gcacaacgga acaaagtgat gtaacagagg tcagcgaggc
    45481 gcacctggca ctcgatgatg cgggcggcac ggcgctggag gagatggaaa agcaaatgat
    45541 ccgcgaggcg ctttcacgcc ataacagcaa gaagcaagtt gctgatgaac tgggcatcgg
    45601 tattgctacg ctctatcgca agattaagaa atatgagttg ttaaacacat aacctcagtg
    45661 aatccggcct gaattcaggc cggatatcct tttacttcgt tttctcgcaa tccagaactg
    45721 tatttacaat catctgatac cccgtgcagc gacaaagatt tcccgccaga ccgcgacgaa
    45781 tttccgtaat ggttaatggc ttctcgcgcg gctttgccag cattgccgtg gtagccataa
    45841 tcaggccagg cgtacaaaac ccgcactgca ccgcgccgga tttcgcataa gcctgctgaa
    45901 catgagaaag ttttccgcct ttcgcttcac cttccagcgt gcggatctct tttccttcag
    45961 cccaggcggc aaggtataag caactgtcta ttgctgtgcc gtcgaccagc accgtacagg
    46021 caccacattc acccacgcag cacccttgtt tgacacttag cagtccttga tcgcggagta
    46081 attccgagag cggcatgcct ggtgcggcgt gaagctgaaa aggcatcccg ttaatggtgc
    46141 attcgatggt aattgtttca ctgtgattca ttgcaatttt ccccccgccg cggcgacggc
    46201 ttcgctaatc acttttttgg tcattgtctg gatgagatgc agacgaaact ctttactggc
    46261 ccgccatgaa gaacgcgggg cgacatcttg caggacagat tcgctgatag cttccagcgt
    46321 ttgcaggttt aatggcgcat tttgtgcagt ctgttcggca tgttggcagc gaatcggcgt
    46381 tggcgcggca acaccaaatg ccaggcgtaa ttcgctgaaa ttgccgctat ccagtcggca
    46441 atgtgcggca cagccaatcg ttgaaatatc cattgcgtcg cgcatggcat atttaaaatg
    46501 cgcgctgccc acgtgttctt tcggctgggg cggaaaatga aaggcgacaa ggatttcgtc
    46561 atgctcaaga gacactttgc ccggcccggt gtgaaagcca ttaatcggga cgaaacgaac
    46621 actgcgtggg gagtggatct ccagtttcgc gtcataaatg agcgttggcg tggcagaatc
    46681 tgcgctggtg gcaccgttgc aaatatttcc accgtaggta gcgacgttac ggatctgcgg
    46741 tccagcaatg gacgaggccg cagcacataa cgccgggaga agacgttgag ttataggatc
    46801 ttctattagc tgggtaaatg tcgttgcaga gccgatacgt agtgagccat cttccgccag
    46861 cgtaattccc cgcagctccg ccagattatg aatatcaaca atatggcgat aacggtcatt
    46921 gtgatggtgg agctgaatca gtacgtcagt gccaccggcg agcagtttgg cctgcgggtt
    46981 gtcagccagc aggttgatgg catcggcaag ggttgctgcg cgatggtaag aagcaaaatc
    47041 aaacatgatg ttatcctcaa atcaatcctg ccagatggaa ctcttcatat aaccgttttg
    47101 gcgtcagcgg cagtgtattg attgcaacac cggtagccat cttcaccgcg ttacgaatag
    47161 cagcggcaac aggaattatt ggtggctcac ctagtgactt atgtccgtat gcggattgcg
    47221 gctcattgat ttcgatgaac gcgctttcca gttgtggcag atccggcatg gtgggcattt
    47281 tgtaatccag cagattgggg ttacggacca cgccgctttt cgcatcgatg atcatctctt
    47341 caaatagcgc ccagccaatg cccattccca ttccgccgtg tacctgacct tctgccagca
    47401 acggattaag aatatggccc gaatcatgaa cgttgaggat gcggttgatg gtgactttgc
    47461 acagcgcaat atcgatcgtc agatcaacaa aggtacagcc aaacgctggt gggttagtgg
    47521 tggttttgat ggagctttcg gcagagagct gcccaccgcg ttcagggtgg tagaaagcgt
    47581 ccatcgccaa atcttttaac gacattaacg gctcttccgg tcgttcaacc agcacgatat
    47641 ggccttttat cagggtcaga ttcatcgctg actgatgtag catgactgcg gcgtgagcga
    47701 tgattttctc ttttaataac agtgccgcac tgcgcagcgc aggcgcggca acatagctct
    47761 ggcgtgaggc aaatgcgccg ggatcgaacg gcgtaacatc ggtatcttgt gttgaaataa
    47821 cgcgaacgtc gctgaccgga acccccacgg tttctgccac catttgcgag aagacggtgt
    47881 cggcaccctg accgatttcc gtcgcgccgc tttgcacgtt gatggttcca tcctgattca
    47941 tcagaaggcg cgcgcctgct atttctacgc cgacaggcca ggtgttcgag gtgtagctaa
    48001 aacaggcgac gccaacgccg cggcgtaaat tgccttgctg gttctggcac tctgcacggc
    48061 gtttttccca ttcaaagatt ttccggcctt tttcaagaca ctccggcaac cctgcgctgt
    48121 aaatacgttt gcccgtgagc ggattagcat ctccttcgcg ggcggcgttg cgtaaacgaa
    48181 tttcaacagg atcaatacct aacgctgtcg cggcgtcatc aagcatagac tcaacggcaa
    48241 atacgacttg tggcgcgcca taaccacgca tcgcaccagc cgaggggagg ttggtatagc
    48301 aggtctttga actgtaagcg taggcacaac gaggataaag gtaagcgact ttattccccc
    48361 ctgcagaagc gatggagtgc ccgtgagatg cataagcgcc ggtgttagac agaacatcca
    48421 gactataacc tttcaatgtt ccgtcgcggt tcacgcccat ttgcccgtca atagtaaaag
    48481 cgtggcgggt acgggttgcg aggaaacact cttcacggct aagggaaact ttcaccggaa
    48541 tgccgccaag cttgctggtc aggaatgctg ccattggctc ttccagtaca tcctgtttat
    48601 taccaaaacc gccaccgaca aacggtttga tgactcgtac gcatgaccag gggatatcca
    48661 gcgcctgacc aaccacgcgg cgaacaatgt gcgggatctg ggtgctggaa acgatggtaa
    48721 ttcgcgagtc atcctccatc catgccagcg atgtcacgct ttccatgtga caatgttgaa
    48781 tgaccggcgt ctgatagtgc ccctgtacct ggtagtcggc ggcatcgatt gtttgttgga
    48841 cattgcccgt cgtcatcgtg ctttgtttta gtaaattgcc gccgttatgg attggttcag
    48901 cgtcatcagc cagcgccgct tctggggtgg tgataacggg taattcttgc cactcaatgc
    48961 tgaccaattg cgccgctttt tctgccgtga gttcatcacg ggccacgacg atggcaacgg
    49021 cgtcaccatg gtgacgaaca tggcgagtta gcagtgcgcg atcggcggta tcgcgcttgt
    49081 tttcgtcaag tgtccaggca tgcccagctg tagcgaatgg gatatcaggc acatcttccc
    49141 aggtaaaaat cgccagtacg cccggtaaac ttctggcttg ttcatcatta atacttacgg
    49201 cataaccatg tgcgataggg ctacgtacat atttcgcgta acacatgccc gccataacat
    49261 aatcgtcagt atatcgtgcc cgcccggtga ccttagcaat ggcatctacg cgcatgcatg
    49321 attcacctgt agcggttgct tcccgcgctt ccatagaaat cccctcgatt tgaacatttg
    49381 gttcttatgg caaagattta gtctaagcag gcaagattta cgccagaaac gacgataagc
    49441 gttaagggag gaaggaagtg tgatgatgtt aaaaaaacac agatatgata cgaaacagga
    49501 ccctgaatag gggcagtgat acgtatcaat atgagtggcg ataaaatcaa tgtgataaat
    49561 tgggattatt acgcatcagg atgggaaata gggtaaatga gtcaggaaat tagatacatc
    49621 tacagtgaat attttgctga tgatacttcc ctttgccaaa tgaaattaac ctctttctct
    49681 attaccgcgt ttttccagaa gcaagagatt gggtatcaag aggctggctg ctatgatatg
    49741 gcgtcttgtt ttttaagcga ggaaagattt tgagtgcggg acgcctgaat aaaaaatctc
    49801 tgggtatcgt gatgttgtta tcggttggac tgcttttggc gggctgctcg ggtagcaaat
    49861 catccgatac aggaacgtat tccggctccg tttacaccgt gaaacggggg gatacgctat
    49921 atcgtatttc gcgcaccacg ggaaccagcg taaaagagct ggcgcgactg aacggcattt
    49981 ccccccctta caccattgaa gttggtcaga aactaaaact gggtggggcg aaaagtagca
    50041 gtacacgtaa atcaaccgcc aaatcaacga ccaaaaccgc atcggttaca ccgtcatcag
    50101 cagtaccgaa atcttcctgg ccgccagtag ggcaacgttg ttggttatgg ccaacgacag
    50161 ggaaagttat catgccgtat tcgacagctg atggcggcaa taaagggatt gatatctcag
    50221 ctccacgggg tacacctatt tacgccgcgg gtgcaggaaa ggtggtgtat gtgggcaacc
    50281 agctgcgtgg ctacggtaat ctcatcatga ttaaacacag tgaagattac attacggctt
    50341 acgcccataa tgacacgatg ctggtaaata atgggcaaag cgtaaaggct gggcaaaaaa
    50401 tcgccactat ggggagcact gatgcggcat cggttcgcct gcatttccag attcgttacc
    50461 gtgcaacggc aattgatccg ctacgttact tgccgcctca gggcagcaag ccaaaatgct
    50521 gatggcgaat taatcagcag tcagcagcgc ggcacttgct aaggagagcg taaggtttat
    50581 aatgccttac gcatctcgaa gcgggcgtag ttcaatggta gaacgagagc ttcccaagct
    50641 ctatacgagg gttcgattcc cttcgcccgc tccaatttat cttcacctca tcaacctgcc
    50701 atttttgttg tatctgattt tctatattat cttaattcaa tcgcttcaga cttttcgcaa
    50761 tcgccgtaat acttccccat ttttttgtaa ataaaagcgt ttacgcaatg caatccattt
    50821 gtaacaaccc aggtaaaata ccttattttc aagttgttaa ataagtggtt atctacgctt
    50881 ttaacaaacg attgatggaa tcttttatga aagggaaatc tacattaact cttctgctcg
    50941 ctggtatatt ttcatgcggc acatgccagg cgacaggggc agaagtgacg agtgaatctg
    51001 tatttaacat tcttaattcc acgggggcag caactgataa aagttattta tcgttaaatc
    51061 cagataagta tcctaattat cgtcttctga ttcactcagc caaattaaaa aatgaaataa
    51121 agagccacta taccaaagac gaaattcaag gattgctaac attaacagaa aatacacgaa
    51181 agttaacgtt gacggagaaa ccctggggaa catttattct tgcttctact ttcgaagatg
    51241 acaaaacagc ggcagaaacc cactatgatg ctgtctggct gagggatagt ttatggggct
    51301 atatggcatt agtatcagat cagggaaata gtgtagccgc aaaaaaggtt ctgctcactc
    51361 tgtgggatta tatgtctacg cccgaccaga ttaagcggat gcaggacgtc attagcaacc
    51421 caaaacgact ggatggtatt cctgggcaaa tgaatgctgt acatatccga tttgacagca
    51481 actcccccgt gatggctgat gtacaggaag aaggcaagcc gcagctgtgg aaccataaac
    51541 aaaatgatgc tttaggtctt tatcttgatc tccttataca agccatcgac actggtacta
    51601 ttaacgctga agactgggaa aaaggtgacc gattaaaatc gattgcgctg ttggttgctt
    51661 atctggataa agccaatttc tatattatgg aagattctgg agcgtgggaa gaagatgccc
    51721 gattaaacac atcttccgtt gcactggtga cctctggact ggaacgtttg tcgaacttac
    51781 tctcgaagaa agactctgtt tttatctctg atttactgcg cgaagctaaa gccaatgaac
    51841 tggatgaacc tctttcaaca acgaggttga accacctcat agataaaggt tatgaaagaa
    51901 taacgctaca acttgattta gggggagaat cccctggtta tctggaaaaa gataaacatt
    51961 acagagaagc tgatgcggcc ttacttaatg ttatttatcc tgcaaatctt tcaaagataa
    52021 acaccagaag aaaagagcaa gttctaaaaa tagtt
//
LOCUS       DACBVI010000015        49059 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.015, whole genome shotgun sequence.
ACCESSION   DACBVI010000015 DACBVI010000000
VERSION     DACBVI010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.015"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..824)
                     /locus_tag="IU325_04495"
     CDS             complement(<1..824)
                     /locus_tag="IU325_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HAP5808299.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWITDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSG"
     gene            complement(821..1849)
                     /locus_tag="IU325_04500"
     CDS             complement(821..1849)
                     /locus_tag="IU325_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HAP5808300.1"
                     /translation="MIISKKQLIGVVAIGIILAGVVFFIWWVSTGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLES
                     IQATIAMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVRAPID
                     GIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGMKPGMKAEIRADILKG
                     EVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLS
                     VTVSVDER"
     gene            1946..2761
                     /locus_tag="IU325_04505"
     CDS             1946..2761
                     /locus_tag="IU325_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP5808301.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGKVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
     gene            complement(2934..3842)
                     /locus_tag="IU325_04510"
     CDS             complement(2934..3842)
                     /locus_tag="IU325_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP5808302.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKHLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKATYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            4018..5328
                     /locus_tag="IU325_04515"
     CDS             4018..5328
                     /locus_tag="IU325_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP5808303.1"
                     /translation="MESLNQFINSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELH
                     QLGYSLALGREVVNESSRMGLPDEFTLQREFERARQQGALEQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEERDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFERAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVPGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            5328..6773
                     /locus_tag="IU325_04520"
     CDS             5328..6773
                     /locus_tag="IU325_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP5808304.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQASCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITDSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYRALSHFGTPEWNCEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            6810..8336
                     /gene="abgT"
                     /locus_tag="IU325_04525"
     CDS             6810..8336
                     /gene="abgT"
                     /locus_tag="IU325_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HAP5808305.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            8347..8862
                     /locus_tag="IU325_04530"
     CDS             8347..8862
                     /locus_tag="IU325_04530"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP5808306.1"
                     /translation="MLRLLEEKIVTPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            9057..9809
                     /gene="fnr"
                     /locus_tag="IU325_04535"
     CDS             9057..9809
                     /gene="fnr"
                     /locus_tag="IU325_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HAP5808307.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            9961..10911
                     /gene="uspE"
                     /locus_tag="IU325_04540"
     CDS             9961..10911
                     /gene="uspE"
                     /locus_tag="IU325_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HAP5808308.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(10961..11218)
                     /locus_tag="IU325_04545"
     CDS             complement(10961..11218)
                     /locus_tag="IU325_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HAP5808309.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            11462..12493
                     /gene="ynaI"
                     /locus_tag="IU325_04550"
     CDS             11462..12493
                     /gene="ynaI"
                     /locus_tag="IU325_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HAP5808310.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPDQGR"
     gene            complement(12544..14157)
                     /locus_tag="IU325_04555"
     CDS             complement(12544..14157)
                     /locus_tag="IU325_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="is involved in the transport of the murein peptide
                     L-alanyl-gamma-D-glutamyl-meso-diaminopimelate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HAP5808311.1"
                     /translation="MKHSVSVTCCALLISSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESSKEWTKPGKLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(14494..15393)
                     /locus_tag="IU325_04560"
     CDS             complement(14494..15393)
                     /locus_tag="IU325_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP5808312.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            15531..16451
                     /locus_tag="IU325_04565"
     CDS             15531..16451
                     /locus_tag="IU325_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP5808313.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVDAGSNARTSDISSGEYATMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPIQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKVYVAEAISVLAPFFEETL"
     gene            16451..16525
                     /locus_tag="IU325_04570"
     CDS             16451..16525
                     /locus_tag="IU325_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808314.1"
                     /translation="MKHIQIRNSDMAWHIAANNLGKVS"
     gene            16522..17160
                     /locus_tag="IU325_04575"
     CDS             16522..17160
                     /locus_tag="IU325_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAP5808315.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            17451..18179
                     /gene="mpaA"
                     /locus_tag="IU325_04580"
     CDS             17451..18179
                     /gene="mpaA"
                     /locus_tag="IU325_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HAP5808316.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSIVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPL"
     gene            complement(18154..19119)
                     /locus_tag="IU325_04585"
     CDS             complement(18154..19119)
                     /locus_tag="IU325_04585"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HAP5808317.1"
                     /translation="MRTVKVFEEGWPLHTPFVIARGSRSEARVVVVELEEDGVKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVTTAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            19238..19744
                     /locus_tag="IU325_04590"
     CDS             19238..19744
                     /locus_tag="IU325_04590"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HAP5808318.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(19788..21329)
                     /gene="tyrR"
                     /locus_tag="IU325_04595"
     CDS             complement(19788..21329)
                     /gene="tyrR"
                     /locus_tag="IU325_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HAP5808319.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(21477..22538)
                     /locus_tag="IU325_04600"
     CDS             complement(21477..22538)
                     /locus_tag="IU325_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HAP5808320.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRVQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(22535..23932)
                     /locus_tag="IU325_04605"
     CDS             complement(22535..23932)
                     /locus_tag="IU325_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HAP5808321.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTEYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            complement(24051..24365)
                     /gene="pspE"
                     /locus_tag="IU325_04610"
     CDS             complement(24051..24365)
                     /gene="pspE"
                     /locus_tag="IU325_04610"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HAP5808322.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(24440..24661)
                     /gene="pspD"
                     /locus_tag="IU325_04615"
     CDS             complement(24440..24661)
                     /gene="pspD"
                     /locus_tag="IU325_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HAP5808323.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(24670..25029)
                     /gene="pspC"
                     /locus_tag="IU325_04620"
     CDS             complement(24670..25029)
                     /gene="pspC"
                     /locus_tag="IU325_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HAP5808324.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(25029..25253)
                     /gene="pspB"
                     /locus_tag="IU325_04625"
     CDS             complement(25029..25253)
                     /gene="pspB"
                     /locus_tag="IU325_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HAP5808325.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(25307..25975)
                     /gene="pspA"
                     /locus_tag="IU325_04630"
     CDS             complement(25307..25975)
                     /gene="pspA"
                     /locus_tag="IU325_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HAP5808326.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDNGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            26142..27119
                     /gene="pspF"
                     /locus_tag="IU325_04635"
     CDS             26142..27119
                     /gene="pspF"
                     /locus_tag="IU325_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HAP5808327.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(27239..28504)
                     /locus_tag="IU325_04640"
     CDS             complement(27239..28504)
                     /locus_tag="IU325_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HAP5808328.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDDHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLTNTL
                     IDAKESVPSIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALND"
     gene            complement(28542..29822)
                     /locus_tag="IU325_04645"
     CDS             complement(28542..29822)
                     /locus_tag="IU325_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP5808329.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(29824..31311)
                     /gene="puuC"
                     /locus_tag="IU325_04650"
     CDS             complement(29824..31311)
                     /gene="puuC"
                     /locus_tag="IU325_04650"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HAP5808330.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADFMEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYTEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLDLLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(31461..32018)
                     /gene="puuR"
                     /locus_tag="IU325_04655"
     CDS             complement(31461..32018)
                     /gene="puuR"
                     /locus_tag="IU325_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HAP5808331.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(32045..32809)
                     /gene="puuD"
                     /locus_tag="IU325_04660"
     CDS             complement(32045..32809)
                     /gene="puuD"
                     /locus_tag="IU325_04660"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HAP5808332.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVL
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            33021..34439
                     /locus_tag="IU325_04665"
     CDS             33021..34439
                     /locus_tag="IU325_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HAP5808333.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASQGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            34588..34752
                     /locus_tag="IU325_04670"
     CDS             34588..34752
                     /locus_tag="IU325_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808334.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            34742..36127
                     /gene="puuP"
                     /locus_tag="IU325_04675"
     CDS             34742..36127
                     /gene="puuP"
                     /locus_tag="IU325_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HAP5808335.1"
                     /translation="MAINSPLTIAAQSGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRAPVSET"
     gene            36261..36506
                     /locus_tag="IU325_04680"
     CDS             36261..36506
                     /locus_tag="IU325_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2543 family protein"
                     /protein_id="HAP5808336.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLALYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            36819..38462
                     /gene="sapA"
                     /locus_tag="IU325_04685"
     CDS             36819..38462
                     /gene="sapA"
                     /locus_tag="IU325_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HAP5808337.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQYIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKPDEVKKP"
     gene            38459..39424
                     /gene="sapB"
                     /locus_tag="IU325_04690"
     CDS             38459..39424
                     /gene="sapB"
                     /locus_tag="IU325_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HAP5808338.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITDFA
                     LIDAWLSDSPWRDEMVMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            39411..40301
                     /gene="sapC"
                     /locus_tag="IU325_04695"
     CDS             39411..40301
                     /gene="sapC"
                     /locus_tag="IU325_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HAP5808339.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLILGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            40301..41293
                     /gene="sapD"
                     /locus_tag="IU325_04700"
     CDS             40301..41293
                     /gene="sapD"
                     /locus_tag="IU325_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HAP5808340.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            41295..42101
                     /gene="sapF"
                     /locus_tag="IU325_04705"
     CDS             41295..42101
                     /gene="sapF"
                     /locus_tag="IU325_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HAP5808341.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(42150..43313)
                     /locus_tag="IU325_04710"
     CDS             complement(42150..43313)
                     /locus_tag="IU325_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family efflux MFS transporter"
                     /protein_id="HAP5808342.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCLLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWTQLPIPALVALFFTIAFCSGIGTVGGAEAMSGVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGFGGETLLKMSLAITVCYTLALLVALTRIDNQK"
     gene            complement(43322..44695)
                     /locus_tag="IU325_04715"
     CDS             complement(43322..44695)
                     /locus_tag="IU325_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HAP5808343.1"
                     /translation="MLRRSLIFLVLLSAGCVSLEPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTETTAEADGTVSSFTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMASAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTHVMQDKNALNLLAGTTLEENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSEALSSLFSHGMQIWSFTPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQAGVGDYLTVLTAQRSLWSAQQALLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(44718..47806)
                     /locus_tag="IU325_04720"
                     /pseudo
     CDS             complement(44718..47806)
                     /locus_tag="IU325_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309891.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            complement(47806..48927)
                     /locus_tag="IU325_04725"
     CDS             complement(47806..48927)
                     /locus_tag="IU325_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAP5808344.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTVKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTVRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
BASE COUNT        11929 a        12831 c        12086 g        12213 t
ORIGIN      
        1 ccagaaaaag aaaaaaaaca tcccagcgtg aaatttttat ccttaaaagc atgaagatcc
       61 attatcggat tggatattgt aagtgtacgc gcagcaaata aaataaaact caccaatgcc
      121 agaactgatg tcagcaaaat agtattatca tccagccatc cccagcgcgc tccttcttca
      181 agagtatatt ccagacatcc cagtgtcatg gccaacagga taatactcgg ataatcagca
      241 actttcagta aagagagatc aggtttatca aaattaacca gaaaaggaat actcaaaact
      301 aaatatatgc caggtaatat attgatataa aatagccatc gccagtcgag gttatcagtg
      361 atccagcccc ccagagttgg ccctagcgtc ggtgacagtg aggccagcgc actgactacc
      421 gcagcagcca accccaactc tttaccctgg taataaataa acgccgtcgt gaaaactaaa
      481 gggatcatgg atgcgccagc cgctccctgt aaagcacgaa acaaaatcat gatctgaata
      541 ttccatgcaa gtccacacgc gatactcatt aaagtaaata ttcccgcgga tagagtaaaa
      601 agccaacgag tggaaaatac ccgtgacagc cacccagaga gcggaataac aataatctca
      661 gcaattaaat agctggtctg aagccaggcc atttcgtctt tacctgcgga cagaccacca
      721 cctatttcat ttagggatga cgatacaatt tgaatatcaa gctgagcaat aaataatccc
      781 atacacatac tggcgaaagc aatatgttta cgcatagaca tcatctctca tcaacactga
      841 cggtgacgga cagtcccggg cgaagaagtt gctttaattc ctttgcgtcg tcaaatgcaa
      901 tccgtaccgg gacacgctga acaattttgg tgaaattgcc agtggcattt tctatcggaa
      961 tcaagctgaa actggctcct gtcgctgggg aaaggctttc aatgtgaccg tgaaacactt
     1021 cccctttgag aatatcagca cggatctcag ccttcattcc tggcttcatt cccgctattt
     1081 ggttttcttt ataatttgca tcaacccaca actcactgac tggtacaagt gaaactaacg
     1141 atgtgccgcc ttccacccaa ctgcctgtat gggcgctgcg gtttgcgaca atgccatcaa
     1201 tcggcgcacg aacaagggta tattccagat ccagtagtgc ctgagtcaaa gcggcctgaa
     1261 cttcttcgat actcgcacgt acgttttctt cctgagcaga taaaactgcc agccgttgct
     1321 tctccaccag gaaatcgttc gcggctttgc gctcctttgc tgcgacctgc tggtagtcaa
     1381 acctggcgtt atcaataatt tgttgcgata ttgccgctga ttgtgcgagt ttttcataac
     1441 gttcagtatc tcgcaggcgc ttttgctcct catgttttac tgcctgccat gtttcactgg
     1501 cactttgaat tatcgactgc tgcatcgcaa tagtggcttg aatgctttcg agattggctt
     1561 tgctactttt tatttttgcc tcaagcctgg cgacatttgc acgataatcc cgatcatcga
     1621 gacgcaggat aatatcgcct ttttttacgc tctgattatc gcgcacctct atagcagaaa
     1681 tataaccaga aactttactt gccaccgtgg tgatattacc accaatgtaa gcatcatccg
     1741 tcgtctgtat aaatcttcct gtgcttaccc accagataaa aaagacaacg ccagctaaaa
     1801 taataccgat cgctaccacc ccaataagct gttttttaga aattatcatc actctttaca
     1861 ccgtatatat ccggaaactt gcattcgtca tatttaaaat ttaacatggc ttatttgatg
     1921 atttctgaca aaatacctac tgtaaatgac aatcacactg caacccaaag catcaccagg
     1981 gcatcacatt atcggactgg attcggaaca tctgaatgga ggtacagttc cctggcataa
     2041 acatctctat gcccagttgc tttacccggc tgaaggagtc gttcgtgtat gggctggaga
     2101 aagcgtctgg ctagtgcatg ccagcagcgc gctttggctg cccccacaaa tgccgcataa
     2161 gtttgttgcg acaggcaacg tacttttaaa aaccgtactg gtttcagaag cggaaagtga
     2221 aacgctggga aaagtttgct tcatgacggg tatttcaccc ctgttaaggg aattactaat
     2281 tgcaattaac caacttccac cgtcgcaaag taccacagat aaacaacagt tacgtttttc
     2341 agccctcgag acactgatac ttcaggaaat aaaaatgggg gtgaaaatgt cacttgaact
     2401 cccttggcca aatgatgagc ggctacagca gctttgtgag aatttgctaa acaaccaagg
     2461 gtatctgcca acattggata atctagcgga taaaattaac gtcagtagcc gtacactcat
     2521 gcgcttattt gttaaagaga cgggattaac atttcgccac tgggttcagc aaatgcacgt
     2581 tatttctgca gtgacgcttc ttgatgatgg atattcgttg accaaaattg cccatcgtct
     2641 gggctatgcc tcagctgaat cgttcggcaa tatgtttaag cgtcgaacgg gctactcgcc
     2701 cggtaaattt actcgtcgat taacgatgca tgattatgca atcacaagac aaatgattta
     2761 acttatattt tcgtgaaatc tgtcactgaa gaaaattggc aacggatggg taaaaccgtt
     2821 ataacactgt caccggtcac caggacccca ggccggattt aagacgagga tgcactgctg
     2881 tgtgtactgt agagtctggc ggatgtcgac agactctatt tttttatgca gttttaactt
     2941 tgcagatagc cgcattctcg ccgaaattgc gtgattaatg acgcggtcag tggtgtctgg
     3001 cgactatcac gtcgctgaat caaataatag gtcgctttcg gtaaaatttc gctaaccggc
     3061 aacatcacca gcccctgtcc gtgcaaggga tcgcagccca tttcttcagg tagtatgctg
     3121 agaaaatcgc tttttgccac cagactgata caggctgaga acgtctcgca gactacgccg
     3181 acctgtggcg tttgcgcctg atcgtcaagc aattcactca agtgtttgta gtagctgccg
     3241 tgtggcgtcg gcattgtcca gctgtagtcc agtaactgtt tgatcgaacg ggcaccaatg
     3301 gcgggatgtc ccgggcggca aaagatcgcg aattgctttt ccagtaattt ctcaaaagta
     3361 aattcgtggt cgtagggtcc ctgataatag gtattgatgg tgaaatccag ttccccctgg
     3421 cgcaattcat taatcatcga taccagttgc ccttccataa tgcgtacttt tacctgcgga
     3481 tgctgctgat gaaaacgaga tatgacagct ggcatcagac tgcgggaaat actggccccc
     3541 atgccgatat taatttgccc tgctagttgc ccttgtcgtt ggcgaatatc ctcttgcgct
     3601 gcgcgcagct cttcaagaat aagactggcg tgctgataaa aactttcgcc ggcatcagtt
     3661 aacgtcacgc ctttactacg gcgaaaaaag agttgtgccg ccaacccttc ttccagctcc
     3721 tgaatagatt tactcagtgc cggttgcgac atattcaaca ttcggctcgc tccgcgaatg
     3781 ctgccctgac gagccacttc aacaaaagcc cgaatttgat gaatttttac ctgaaaagcc
     3841 atgacgccac cgataaccgt tatttatcag accaaagaaa ctggcatcta ctttaatgca
     3901 gatgattgtg tcagggtaat ttatgaacgg ttaaaactgt gaaaaatcag ttagtgataa
     3961 gtaaaaacta tcgctacgtg aaccgggtca cactttttac tgatgacggg aaaggttatg
     4021 gagtctttga atcaatttat taattcgctt gccccaaaat tatcgcactg gcgacgtgat
     4081 tttcaccact atgcagagtc tggctgggtg gaattccgca ctgccaccct tgttgcggaa
     4141 gaattgcacc agctcggtta ttcactggcg ctgggccgcg aagtagttaa tgaaagtagc
     4201 cggatgggat tacctgatga attcactcta caacgcgaat tcgagcgcgc tcgtcaacag
     4261 ggtgcgctag aacaatggat tgcggctttt gaaggcggtt tcactggcat cgtcgctacc
     4321 ctggataccg gtcgccccgg tccggtgatg gctttccgtg tcgatatgga cgcgctggat
     4381 ctcagtgaag aacgggatgt cagccatcgc ccctaccgcg acggttttgc gtcatgtaac
     4441 gccggaatga tgcatgcctg tggtcatgat ggacataccg ccattggact tgggctggcg
     4501 cataccctta aacagttcga gtccggacta catggcgtca tcaaactgat ttttcaacct
     4561 gcagaagaag gtacgcgtgg cgcgcgggcg atggtcgatg caggtgtagt ggatgatgtt
     4621 gattatttta ctgccgtgca cattggcact ggcgtacctg cgggcaccgt ggtgtgcggc
     4681 agtgataatt ttatggcaac caccaaattt gacgcgcact tcaccgggac cgccgctcac
     4741 gcaggcgcaa aaccagaaga cggtcacaat gccttgttgg cggcagcaca agcaactctt
     4801 gcactgcatg caatcgcccc gcacagcgaa ggcgcttcca gagtaaacgt gggcgttatg
     4861 caggcaggaa gcggtcgtaa tgttgttccc gcctcagcgt tgctgaaagt ggaaacacgc
     4921 ggggccagcg acgtcattaa tcaatatgtt tttgaacgtg cacaacaagc gattcagggc
     4981 gcagcaacca tgtatggtgt cggcgttgaa actcgtctga tgggtgcagc taccgccagt
     5041 tctccttcgc cgcaatgggt cgcatggttg caaagtcagg cagctcaggt cccgggggtc
     5101 aatcaagcca ttgaacgtgt tgaagcgcct gcgggttccg aagatgccac attaatgatg
     5161 gcccgcgtgc agcaacatca agggcaagcc tcctacgtgg tgtttggcac acagctggcg
     5221 gcaggtcatc acaacgaaaa attcgatttt gacgagcagg ttctcgctat tgccgtcgaa
     5281 acgctggcgc gcaccgcgct caattttccc tggacgcgag gtatctgatg caggaaatct
     5341 atcgttttat cgacgatgcg attgaagccg atcgccaacg ttataccgat attgccgatc
     5401 aaatctggga tcatccagaa acacgttttg aagagttctg gtcagcggag catctggctt
     5461 cggcgctgga gtctgcaggc ttcaccgtta cccgcaacgt aggcaatatc ccaaatgcct
     5521 ttattgcttc gtttggtcaa ggcaaaccgg ttatcgccct gctgggggaa tatgacgccc
     5581 tggcaggttt aagtcagcaa gcaagttgcg cgcaacctac atccgcgacg cccggtgaaa
     5641 atggtcacgg ttgcggacac aatttgctgg gaaccgccgc ctttgccgct gcaatagccg
     5701 tcaagaaatg gctggaacaa tatgggcaag gcggcacggt gcgcttttat ggttgtcctg
     5761 gcgaagaagg cggctcgggt aaaacgttca tggtccgcga gggggtattt gatgatgtgg
     5821 atgcggcact cacctggcac ccggaagcct ttgccggtat gttcaatacc cgcacgctgg
     5881 caaacattca ggcatcatgg cgctttaaag ggatcgcagc acatgccgcg aattcccctc
     5941 atttgggacg cagcgccctt gatgccgtaa cgttgatgac cactggcacc aacttcctca
     6001 acgaacatat tattgaaaaa gcgcgcgtac actatgccat cacagatagc ggcgggatct
     6061 cgcccaacgt ggtccaggcg caggcagaag tgctttatct tatccgcgcc cccgaaatga
     6121 ccgatgtgca gcatatttat gatcgggtcg ccaaaatcgc cgaaggtgcg gcattgatga
     6181 ccgaaaccac ggttgaatgc cgcttcgaca aagcctgttc cagttatctc ccgaatcgca
     6241 ccttagaaaa tgccatgtac cgagccctat cccattttgg taccccggaa tggaactgcg
     6301 aagaactggc ttttgcgaaa caaattcagg ctacgctcac ccccaacgat cggcaaaaca
     6361 gtctgaataa tatcgctgca accggtggcg aaaacggcaa ggcttttgca ctacgtcatc
     6421 gtgaaacggt actggcgaat gaagtcgctc catatgccgc caccgataac gtgcttgcgg
     6481 catcgactga tgtcggcgac gtcagttgga aactgcctgt tgcccagtgt ttcagcccct
     6541 gctttgccgt tggtaccccg ctacatacgt ggcaactggt tagccagggg cgaacatcta
     6601 ttgctcataa aggaatgctg ctggcggcga aaactatggc agcaaccaca cttaatctct
     6661 tcattgattc agggctattg caagaatgcc aacaagagca tcagcaagtt acggacacgc
     6721 aaccgtatca ctgccctatc ccgaaaaacg tgacaccgtc acctttaaaa taacaacaac
     6781 aacgcaaaca caacaaacga ggaatgccca tgagtatgtc atccataccg tcgtcctccc
     6841 aatccgggaa gctctatggc tgggtcgaaa gaattggtaa caaggttccc catccttttt
     6901 tgctctttat ctatttgatt atcgtactca tggtgacgac ggcaattttg tcggcctttg
     6961 gcgtcagtgc aaaaaacccg accgatggta cgccggtggt ggtgaaaaac ctgctcagtg
     7021 tggaaggatt acactggttt ttacccaatg ttattaaaaa ctttagcggt tttgctccac
     7081 ttggtgcgat cctggcgctg gttttaggtg ccggtctggc ggagcgcgtc ggcttactac
     7141 cagcgctaat ggttaaaatg gcatcgcatg ttaatgcccg ctacgccagt tatatggtgc
     7201 tgtttattgc ttttttcagc cacatttctt ccgatgcggc gttagtgatc atgccaccga
     7261 tgggtgcgct aatttttctg gcggtgggca ggcatccagt tgcaggttta cttgccgcca
     7321 ttgcaggcgt aggttgcggc tttacggcta atttactgat tgtcacaacc gacgtgttgc
     7381 tgtcggggat cagcacggag gctgcggctg cgttcaatcc gcaaatgcac gtcagtgtaa
     7441 ttgataactg gtattttatg gccagctccg tagtcgtact gacgattgtt ggcggcctga
     7501 taaccgacaa aatcattgag ccacgtttag gtcaatggca gggaaacagc gatgagaaac
     7561 tgcagacatt gaccgaaagt cagcgttttg gtttacgcat agcaggtgtc gtatcgctac
     7621 tttttattgc tgcgattgcg ctgatggtga tcccggaaaa cgggatattg cgcgatccga
     7681 ttaatcacac cgtgatgcca tcccccttta ttaaaggtat cgtgccactg atcattcttt
     7741 ttttctttgt ggtctcgctg gcttatggca tcgctacccg cacaattcga cgtcaggcgg
     7801 atttaccgca tttaatgatt gaaccgatga aagagatggc gggatttatc gtgatggttt
     7861 ttcccctcgc ccagtttgtc gccatgttta actggagcaa catggggaaa ttcatcgccg
     7921 tggggctgac cgatatactg gaaagttcag ggcttagcgg catcccggcg tttgtcggtc
     7981 tggcgttgct ttcctctttc ttatgcatgt ttatcgccag cggttccgca atctggtcga
     8041 ttctggcccc cattttcgta ccgatgttta tgctacttgg ctttcacccg gcatttgcgc
     8101 aaatcctctt tcgtattgcc gactcatccg tattgccttt agcgccagta tctccttttg
     8161 ttccactgtt tcttggattc ctgcaacgct acaaaccaga cgcgaaactg ggtacttact
     8221 attcgttagt tttgccctat ccacttatct ttttggtggt atggctgctg atgttgctgg
     8281 cgtggtatct tgtcggtctg ccgataggtc cggggattta cccacgtttg tcttaagaga
     8341 gaacggatgc tgagattact tgaagaaaaa attgtcacgc cactgggtcc actgtgggtg
     8401 atttgcgatg agcaatttcg cctgcgggcg gttgaatggg aagagtacag cgaacgcatg
     8461 gtgcagctgc tggacatcca ttatcgcaaa gaaggctatg agcgcatttc tgccaccaac
     8521 ccaggcggtt taagcgacaa acttcgtgat tattttgccg gtaatcttag cattattgat
     8581 acgcttccca ctgccacagg ggggacgcca tttcagcgcg aagtctggaa aacactacgc
     8641 actatcccct gcgggcaggt aatgcattac ggtcaactgg ccgaacaatt gggtcgccct
     8701 ggcgcggcgc gtgccgttgg tgcggctaac ggatcgaatc ccatcagcat tgttgtgcct
     8761 tgtcatcggg tcattggcag aaacggtacc atgaccggat atgcaggcgg agttcagcga
     8821 aaagagtggt tattgcgcca cgaaggttat cttttgctgt aaacattaaa caatttgtgc
     8881 cagcttgttc acacttttat gtaaagttac ccttaacaac ttaagggttt tcaaatagat
     8941 agacatatat ttacatctaa tatcggaatt ctctgctgtt aaggtttgct tagacttact
     9001 tgctccctaa aaagatgtta aaattgacaa atatcaatta cggcttgagc agacctatga
     9061 tcccggaaaa gcgaattata cggcgcattc agtctggcgg ttgtgctatc cattgccagg
     9121 attgcagcat cagccagctt tgcatcccgt tcacactcaa cgaacatgag cttgatcagc
     9181 ttgataatat cattgagcgg aagaagccta ttcagaaagg ccagacgctg tttaaggctg
     9241 gtgatgaact taaatcgctt tatgccatcc gctccggtac gattaaaagt tataccatca
     9301 ctgagcaagg cgacgagcaa attactggtt tccatttagc aggcgatctg gtgggatttg
     9361 atgccatcgg cagcggtcat cacccgagct tcgcgcaggc gctggaaacc tcgatggtat
     9421 gtgaaatccc gttcgaaacg ctggacgatt tgtctggtaa aatgccgaat ctgcgtcagc
     9481 agatgatgcg tctgatgagc ggtgaaatca aaggcgatca ggacatgatc ctgctgttgt
     9541 cgaagaaaaa tgccgaggaa cgtctggctg cattcatcta caacctgtcc cgtcgttttg
     9601 cccaacgcgg cttctcccct cgtgaattcc gcctgacgat gactcgtggc gatatcggta
     9661 actatctggg cctgacggta gaaaccatca gccgtctgct gggtcgcttc cagaaaagcg
     9721 gtatgctggc agtcaaaggt aaatacatca ccatcgaaaa taacgatgcg ctggcccagc
     9781 ttgctggtca tacgcgtaac gttgcctgat ttttccgcat aactcactat ccttctgtca
     9841 tatcattaaa tttttctgat ttattgatct ggcagaaggt tcatcactgt ttcattcacc
     9901 agatatgggt taatctttta attacaaact gcgttgacag ttgttgtaag gagaccctgt
     9961 atggctatgt atcagaacat gctcgttgtt atcgatccta accaggacga ccaaccagca
    10021 ttgcggcgag ctgtttattt acatcaacgg attggtggca aaattaaagc ctttttgccg
    10081 atctatgact tctcatacga aatgaccacc ctgctctctc cggacgaacg taccgctatg
    10141 cgtcagggcg tcatcagcca gcgtacagcc tggatccacg agcaggcaaa atattatctc
    10201 aatgctggcg ttcccattga aattaaagtg gtctggcata accgtccttt cgaagccatc
    10261 attcaggaag tgatcagcgg cgggcacgat ttggtgctaa aaatggcgca ccaacatgac
    10321 cgtctggaag cggtgatttt tacgccaacg gactggcatc tgttacgcaa atgcccaagc
    10381 ccggtgtgga tggtgaaaga ccagccgtgg ccggaagggg gtaaggcgct ggtggcggtg
    10441 aatctcgcca gtgaagagcc gtaccataat gcgctcaatg aaaaactggt caaagagacg
    10501 atcgaactgg cagaacaagt caaccatact gaagttcatc tggttggcgc ttatccggta
    10561 acgccaatta atatcgcgat tgaactgccg gaatttgatc cgagcgttta taacgatgcc
    10621 attcgtgggc aacatttgct ggcaatgaaa gccctacggc agaaattcgg cattaatgaa
    10681 aacatgacgc acgtagaaaa aggtctgcca gaagaggtga ttcctgattt ggcggagcat
    10741 ttacaggcgg ggattgtggt tctgggcacg gtcggacgca ccggtatttc agcagcattc
    10801 ctcggcaaca cggcggaaca ggtgattgat catcttcgct gcgacctgct ggtcattaaa
    10861 cctgaccagt atcagacacc cgttgaactg gatgacgaag aagacgatta acgactcttc
    10921 aataactgac aaggcccgag ttatcgggcc tattttttac ttactgctct tcgccgccaa
    10981 gaaaataaat tcccaacggt attgccagca acacagtaaa gaccaggcta taaacaaaaa
    11041 tcatcgatga ctgaatcaca tacattgatg tcgtccactc agacagcgga atattgtact
    11101 gttctataac gccgccgatg gtcgcgcgag tcacaaccga cgccgcaata atgaaaaccg
    11161 ccaacaaacc aaagagaaat ttcttccctt tcgctgactt cagtttcgcc ataatcatga
    11221 tgtaatcctt gtgcagacaa atttcttgtt acctaaacgt aacaatacca tgacatttta
    11281 ccgttgtctg ctctgaaaac cttacctctt agctggattt acaatcaacg cattgattag
    11341 ttagctaatg ttgctacttc cttccctgac tattgatgat ctgaatgaat tgtcgcggta
    11401 aaatagcccc gccaggggtt tccggcatga cgacaattat ctggaggagc ctgataaaac
    11461 aatgatcgct gaactgttta caaataatgc gcttaatctg gtcattattt tcggtagctg
    11521 cgcagcattg attctgatga gcttttggtt tcgccgcgga aatcgtaaaa gaaaaggttt
    11581 tttattccat gcggtgcaat ttttaatcta caccataatt atcagtgctg ttggtagcat
    11641 cattaattat gttatagaaa actataaact caaatttatc accccaggcg ttatcgattt
    11701 tatctgtacg tccctgattg cggttattct gacgattaag ctatttctgc tgattaatca
    11761 gtttgaaaaa cagcagatta aaaaaggtcg cgatatcacc agtgcgcgga ttatgtcgcg
    11821 tatcatcaaa atcaccatta ttgtggtgct tgttctgctt tatggcgaac atttcggcat
    11881 gagcctttct ggcttgttga cctttggtgg tattggtggt ctggctgtcg gtatggccgg
    11941 taaagatatt ctgagtaact tcttttccgg gattatgctc tatttcgacc gtccttttag
    12001 tattggcgac tggatccgtt caccggacag aaatatcgaa ggtacagtag cggaaattgg
    12061 ctggcgaatt accaaaatta cgacctttga taatcgtcca ttgtacgtac cgaactcgct
    12121 gttttcgtcg atcagcgtag aaaatcctgg acgaatgacc aaccggcgca ttaccacgac
    12181 cattggttta cgttatgagg atgcggcaaa agtgggcgtt attgtcgaag ccgtacgtga
    12241 gatgctgaaa aatcacccgg ccatcgacca gcgacaaacc ttactggttt atttcaacca
    12301 gtttgccgat tcgtcattaa atattatggt ttattgcttt accaaaacca cggtatgggc
    12361 tgaatggctt gctgcacagc aagacgttta tttgaagatt atcgatattg tacagtcaca
    12421 cggcgcggac tttgccttcc ccagccagac gctgtatatg gataacatta caccgccgga
    12481 ccagggtcgc taaatcagac atcactggcg ttatccacaa gggtaacgcc aattcccatc
    12541 acatcaatgc ttcacaatat acatagtccg actgtacgcc acatcttccg ggttattaat
    12601 gggataacct ttcagccacg gcttgattaa tcgtccattg gtatattgat aaattggtgc
    12661 aatcggtgct tgctccatga ggattttttc tgccgcattg taatcggcat tacgcgcttt
    12721 aacggtattt tccgttgatg cctgggccag tactttgtcg tatgccggat tgttaaagcg
    12781 tgaaatattc cctgaatgcg ttgacgttaa caacgtaagg aaagtggagg gttcattata
    12841 atcccctacc catgaggcgc ggataacatc aaaattgccg gtgttacggc tatcgatata
    12901 ggttttccat tcctgattct gcaatttaac atctacgcca aggttctttt tccacatcga
    12961 tgctacagca atcgcaattt tttgatggtt ttctgaagtg ttatacaaaa gcgtcagctt
    13021 cagcggtttt tgcggaccat aaccagcggc gctcaacaaa gtttttgcct gcgcattcag
    13081 ttcttcctga ctcatttgtt caaacggcga aggctccggc gtaaaaccgg cagtaacatc
    13141 tggtgtaaaa tgccacgctg gcttttcccc cgtccccaac actttttcgg tcatcaggcg
    13201 gcgatctatc gtcatactta atgccaggcg aacgcgctga tcggccgtcg gccctttttg
    13261 cgtgttaaac gcataataat aggtcccgag ctgcggcggc gtataaacct gccccggaat
    13321 atccttcaac agcttctgat acatattttt cgggaaggat tcggtgatat caatatcccc
    13381 cgcgaggtaa cgcttagtgg ctgcggattc ctgattaatt ggcaggaagg tcactttttg
    13441 cagtaccgtt ttggcgttat cccaataatg ggtattcggt accacgacca gtttttcatt
    13501 gactacgcgc tctttaagaa cataagcgcc attgccgatc agttttccgg gtttcgtcca
    13561 ctctttactg ctttctacgt tggctttttg caccgggaag aaggcaaagt tagcggttaa
    13621 attcacaaac cacggcaacg gtttatcaag ctgaattttc aaagtatggg catcaactgc
    13681 ggtgacgcca agctggtcag gcgtagcttt accatcaata atcgcctgtg cgttgttgat
    13741 tcccgccagc gcggcaaacc atgcaaatgg cgatagcgtt ttcgggtcca ccagacgctg
    13801 ccagctgtag acaaaatctt gcgccgttac cggtgtgcca tccgcccatt ttgcgttatc
    13861 gcgcagggta aaagtccaga tacggttgtc attacttttc cactgagtcg caacgccggg
    13921 gacaatctcc cctttctcgt tctgattcac cagtccttca aacagatcgc gaatgacctg
    13981 aatctctggc aggcccacgg ctttagcggg atccagcgac gcaggctcat ctttaatgtg
    14041 gcgcaccaac tcctgcttct ctgccagtac tgtgccgctc ggaacttctg cagcatacga
    14101 aagagaaatg ctgctgatca acagcgcaca acacgtgact gaaacagagt gcttcataag
    14161 tttccctcta accgacatta aaattgatgc gtaattattt gtttcgcctt aaagaaatgc
    14221 aaacaacttc catcaaaaaa gtgacaaagg aagcatcaaa tgttttaagt atcaatatat
    14281 caaatcagat ttagtgtaaa taatagtctc caaaatgcga taaagcacat agaactccga
    14341 acactacgtt cgggcttcgc acgttcaaac ccgttttcgg tatcataatc agcatctgtc
    14401 gccattgtgc gaagaataaa aaataaccaa aacgttgaaa ggaactgaga atacgttaag
    14461 agaagaatta aattaatgta tcagcaagta gtgctaaaca gcacctttat acttcagcct
    14521 atctataaat aatgagaatg ccgagccagc atgtcgacgg tgtggatagt acaggtgata
    14581 accgggtaaa tctggtgtga atttatccaa aacacgtatc agtttttttt ctttaatagc
    14641 ccgctcaacc tgatcataag gtagatacgc caatccatgc ccatcaattg cagcatcaat
    14701 gatcaggtct atcgtattca gtaaaagctg accttccatg cgaacacgaa cttcacgtcc
    14761 accgcgtatt aatctccagc gatttgctgt acccgatgtg ggaagataca aattaattgc
    14821 ctgatgatct attaattgtg acactgacgt tggagcactt cgacgagaaa aataatccgg
    14881 tgagccaaca atagccattg gtatatctgg cccgattcga atggcgatca tatctttatc
    14941 catctcccca cccagacgga cgcctgcatc aaaacgttca gaaacgacat cggtcaaacc
    15001 ataatcaatg gtaagctgga tatcaatttc aggatgcgat ttcaggaatg tgcgcatagc
    15061 tggtaacaat atcgtttttg ctgcatgttc tacagtagta atacgtattg tcccggatgg
    15121 gcggttctgc agatcgctca gggatgtcag ggcagaatct atgtcatgca acatagggcc
    15181 aagaacagac aaaagatgct cgcccgcttc agtgggtaca acgctgcgcg tggttcgcgt
    15241 cagaagccgc aatcctaatc gttcttctat acggcgcact atctggctta aagctgattg
    15301 cgccatgctc aggcgggctg ctgcgcgagt gaagctacgc tcctctgcaa cgacgacaaa
    15361 cgccatcaga tcagcgattt cttcacgctt cattcagtat ctccatacaa tctattaatc
    15421 atttaaatga taggtctaat cataatttag ggtctaatca acttaatgat caatatctat
    15481 agtgatatca ccaaccgcca aaacgaaccg acaactccat gaggaacgga atgatgaata
    15541 ataaagtcag cttcactaac agcaataatc caaccatctc tttgtctgca gtgatctatt
    15601 tccctccaaa atttgatgaa acccgtcagt atccggcaat tgtggtatct cacccaggtg
    15661 ggggcgttaa agagcaaacg gccggaacct atgccgaaaa actggcggaa aagggatttg
    15721 tcactattgc ttatgacgca tcttatcaag gtgaaagcgg cggcgagccg cgtcagttag
    15781 aaaacccata cattcgtact gaagacgtca gtgccgtgat tgattacctg acaacgcttt
    15841 cttatgtcga caatacccga attggtgcga tgggaatttg tgccggtgca ggatatacag
    15901 ccaatgctgc aattcaggat cgccgcatca aggccatcgg taccgttagt gcggtcaata
    15961 ttggttcgat gttccgtaat ggttgggaaa acaatgtgaa atctattgat gccctaccat
    16021 acgttgacgc gggttcaaac gcccgaacca gcgatattag cagcggagaa tacgccacca
    16081 tgccactggc accaatgaag gagtccgatg cgccgaacga agaactgcgt caggcctggg
    16141 agtattacca cacccctcgc gcgcagtatc caacagcacc gggttacgct actttgcgca
    16201 gccttaacca gattattacc tatgatgctt accatatggc ggaagtgtac ctgactcagc
    16261 cgatacagat tgtggcgggc agccaggcag ggagcaaatg gatgagtgac gacctgtacg
    16321 atcgagcatc aagccaggat aaacgctatc acattgttga aggcgcaaac catatggatc
    16381 tttatgacgg caaagtctat gtggctgaag caatttcagt attagcgccg ttctttgagg
    16441 aaactctgta atgaaacata ttcaaatccg taatagtgat atggcctggc atattgctgc
    16501 caataatctg ggaaaagttt catgaaaaat gtactgattc ttggtgctgg cggtcagata
    16561 gcccgccatg tgattaatca actggcagat aaacaaacca ttaaacaaac tttatttgcc
    16621 cgtcagccag caaaaatcca caaaccctat ccaactaata gcaagattat tatgggggat
    16681 gtactgaatc atgcagcact gaagcaggcc atgcagggac aggatgttgt gtatgccaac
    16741 cttacggggg aagatttaga tattcaggca aattcagtga tcgctgcgat gaaagcctgt
    16801 gatgttaaac gtctgatctt tgttctttcc ctcggtattt atgatgaagt tccaggaaaa
    16861 tttggcgaat ggaataatgc agttattggt gaacccctaa aaccattccg cagggctgcg
    16921 gatgcaattg aggcttctgg actggaatat acaattcttc gccctgcctg gctgactgat
    16981 gaagatatta ttgattatga gcttacctca cgcaatgaac cattcaaagg tacgatcgta
    17041 tcacgtaaga gtgtcgcagc attaattaca gatatcatcg ataaacctga gaaacacatt
    17101 ggtgagaata taggcatcaa tcaacccggg actgatggcg ataagccatt cttcatgtaa
    17161 atcaattata tagctaccat cgtactgtac tatgtgcagt acgatttatt aaacttatta
    17221 aggtgaactg atcaatcaga tgcgattaac atgtgtagaa ccagtaagaa taaaatacgc
    17281 catgtatata acattacgac aattctaata tctgcacatc aatgaaaatg caaacaactt
    17341 ccggcaaaaa agtgatacaa gatcgatgac atagcgttga agaaaaggat ttgataatcc
    17401 gcaaaatttg cacaacactg cctgtaatac ttcctgaacc agagacgatc atgaccgtaa
    17461 ctcgcccacg cgccgaacgc ggcgcatttc cgcccggaac agaacattac ggacgttcat
    17521 tattgggcgc gccgttaatc tggtttcctg cccctgcagc tagccgcgaa agtggtttga
    17581 ttctggctgg cactcacggt gatgaaaact cttcgatcgt cacgctctcc tgtgctctgc
    17641 ggacgttgac accttctttg cgccgccatc atgtggtgct gtgtgtgaat cctgacggct
    17701 gccagttagg gttacgggcc aatgctaatg gtgtggattt aaacagaaac tttccggcgg
    17761 cgaattggaa ggaaggtgaa accgtttatc gctggaacag cgccgctgaa gaacgtgatg
    17821 tcgtattgct gacaggtgac aaacctggct ctgaacctga aacccaggcg ctgtgccagc
    17881 ttatacaccg cattcaaccc gcctgggtgg tctcattcca cgatccgctg gcctgtattg
    17941 aagatcccag acacagtgaa ttaggcgaat ggctggccca ggcgtttgaa ttgcctctgg
    18001 taaccagcgt cggttatgaa acacctggct ctttcggtag ttggtgtgcg gatctcaacc
    18061 tgcattgcat caccgcagag tttccgccca tctcctccga cgaagccagc gaaaaatatc
    18121 tctttgcgat ggcaaacttg ctgcgctggc atcctaaaga tgcaattcgc ccgttgtgaa
    18181 ctgaagtgcc ggttccacat ctaccgccag ccaggtcggt ccgtcaagat cggcgaaact
    18241 gacctgcggc accagcggta aagcggcgct aatggcacga gaggtacaca acatgcagcc
    18301 cagcatcaga ctaaaacctt gtgcacgtgc ttcagtcgcc agcgccagcg cttccgtcag
    18361 gccacccgtt ttatcgagct taatgttaac catctcatag cgccctttca gcgccttcag
    18421 attgctacga gtatgacaac tttcatctgc acaaatcggt aacggatgaa taaaattctc
    18481 caacgccgca tcgtcctgcg caggaagcgg ttgttcaagc atcgcaacgc ctaaatccgc
    18541 caatagctgg caacgcgccg ccaacccttc tgcacgccag gattcatttg catcaacgat
    18601 cagcgtcgca tcgggcacag cggtgcgaat tgccaccatc cgctcactga taagatggtt
    18661 atccagcttc actttcagta atttcgcgcc tgcctgccag agtgttgatg cactattggc
    18721 catctgatca ggcgtaccga tgactaccgt ctgtgcagtg gtgactgtct cgggaagcgt
    18781 tatgccgatc aaatcagcca gcgattgctg ctgttttcgc gccgccagat cccacaatgc
    18841 acaatccagc gcattacgtg ctgcgccggc agggagaatt ttttgcaact cctcccgtgt
    18901 cagccctttc tctaattgcg gcacgacgct cataatttgc gccattaccg aggcatcact
    18961 ttcgccatag cgcggataag gcgtacattc gcccgtacct ttgacgccat cttcttccag
    19021 ttcaaccacc accacgcgcg cttcactgcg acttccccga gcaatcacaa acggggtatg
    19081 taagggccag ccttcctcga ataccttaac ggttctcatc tttactcctt gttgtcgcgg
    19141 tggtctgcgg attaagcgca aatagcgttt gctgtgttat tgacagttag cataaactag
    19201 gtgtgacgtt aactatatgt aaacaggaaa gataatcatg tcacaaaccg ttcatttcca
    19261 gggcaacccg gttacagtcg ccaattccat cccgcaggcg ggtagcaaag cgcagacttt
    19321 tactctcgtg gcaaaagatc tgtctgacgt caccctcggt cagtttgcgg gtaaacgcaa
    19381 agtgctgaac attttcccga gtattgatac cggtgtttgc gccgcatcag tacgtaagtt
    19441 taatcaactg gcaaccgaga tcgacaacac cgttgtgctg tgtatctctg ccgatctgcc
    19501 gttcgcccag tctcgtttct gcggcgcaga aggtctgaac aacgttatca ccctctccac
    19561 tttccgtaat gctgaattcc tgcaagccta cggtgtggca attgctgatg gcccactgaa
    19621 aggtctggca gcgcgtgccg ttgtggttat tgatgaaaat gacaatgtga ttttcagcca
    19681 gctggtggat gaaatcacca ccgagccgga ttacgaagca gctctggctg tactgaaagc
    19741 ataagttaat atgcctgatg gtgttgcacc atcaggcata ttcgcgctta ctcttcgttc
    19801 ttcttctgac tcagaccgta ttcccgcaac ttattggcaa tcgcggtatg cgaaacgccc
    19861 aggcgttttg ccagtttgcg cgtgctggga taattgcgat aaagctgggt taataccgag
    19921 cgttcaaaac ggctggtgat ttcgtccagc gaaccttcca tcgcatcttc gcccacggct
    19981 accgttgcgg cgtcataatc cggtaacaaa atatcctgtg gacgcagctc ataaccgtcc
    20041 agttgtgtca gtgcgcgata aatagcgttc tttaactgcc gcacatttcc cggccacgca
    20101 taacgcgtaa gtacagtatt caggtcagcg gccagtttcg gacgcggcac gccctgctcg
    20161 tcggcaaagc gggcgacgaa cagctcggtt aacggcatga tgtcctgcgg acagtcacgt
    20221 agcggcggca gattaagcgt caacacgttc agacgataat aaagatcttc acggaacacg
    20281 cctttttgca ccagttcgac aagattcttc tgcgtcgcgc aaatcacccg cacatcgaca
    20341 tgcacctcat ggtcttcgcc aacccgacgg aaagtgccat cattaaggaa acgcagtaat
    20401 ttcgcctgca tccgtggtga catctcacct atttcatcca acagcaccga accaccgttc
    20461 gcctgctcaa agaacccttt cttcccttcc ggagcatgac caaacagttc actctcgacc
    20521 gcatcttccg gtatagacgc acagttcagc gccagatagg gtttgcccgc gcgtgggctt
    20581 gcctgatgac aggcatatgc aaagagatct tttccggtgc cggtatcacc ggttatcagc
    20641 aatggcgcgc ttagcatcgc cagtttctgc gcctgttcga caacatgctt cattttcggg
    20701 ctgacggcga caatttgact gaaggcgctg acgtcctggg cggcgacatt ttgcaactga
    20761 cgacccatac gaatcgttga tcgcaacatc accaccgcac cggtcaggac gtgttgatca
    20821 ttttcatcct gaagataaac aggcgtaatc tccatcagga aattctgtcc attaataacg
    20881 acatgctcgt tatgggaatc ctgcggttcg ctttccagcc agcgtaaaaa attaaagccg
    20941 ttaatcaatt gcgcggcggt atggttgcgc aggcgatcca atttttgccc aaaaagctga
    21001 cagctcgccg ggttcgccat atccactttg cttttcatat cgacagagag cacaggttca
    21061 ggcaacgctt ccagtaacgc gctcaacgcc agatgctcac gttcagaagg catccacggg
    21121 acagtacgga catcggtaac acctgcaata cggcgtattt cggccatcag actgctgaaa
    21181 ctctcaaact ccagttcagc aaaattgagg tagattcgcc caatgggatc aatctcaata
    21241 ccgcgtaaat caatgcctct tagcacgagt agatcgagta attcgcgggt cagaccgagt
    21301 cggtcttcac aaaagacttc cagacgcatg ggaaccttca cctgaaaaaa gaacaattaa
    21361 tatgatgata tgacactatt tggatagcag gaaggtttct gtcaacaatc attgacacaa
    21421 agacggaaaa acgggcactt cggcgtaaag atatccggtc agcacccgat attgcattat
    21481 ttttcgctgg gcgttttgcc tttttgcaga gtttctttca cctgaccgat aagctgacga
    21541 cggaaatccc cgaggcgtgg cttgtcatcg tcaatccacg gcagcgggcg gcaaagctcc
    21601 atagctttaa tccccagtcg tgccgtcaga agtcctgcac caatcccctg agctgcgcgg
    21661 gtagacaaac gcgcagcgag atcttgcgac atccagtcca tccccacttc gcgaaccagt
    21721 tcgctggctc cggcgaaagc gatattcagc aataccagct tgaacaggcg caaacggctg
    21781 taatacccca gttcaatgcc atacagcgtg gcgatgcgat taatcaaacg cagattgcgc
    21841 caggcgataa atgccatatc caccagcgcc agcgggctaa cggcgatcat taacgttgat
    21901 tccgctgccg aacggctgat ttcgcgccgt gcctgggcat ctaaaaccgg ctggaccaga
    21961 tgagcataca ggctgaccac ttcacgatcg ttctgcgttt catggattga ggcataccag
    22021 cgttgcagcg ccggatgcga ctgatcaata cccgcctgct gcgccagttt ttcgcagaat
    22081 gcacggcctt tacccgtgcc gtgactgtgc aacaaatcgc gcgcttcgtc gcgttcatgg
    22141 gcgcgctgtc gcaagcgcca taagcgccgc cactctgtta ccacagaacc tacgccagcg
    22201 ccgataatca atgccccagc ggcacatcca cccagcgcca cccagtcctg ggtttgccag
    22261 gcgttcattg tccactgtac accctgcccg acaacgcttg cgccaaacag agccaacccg
    22321 cccatcacca ttttgcgcca caggctgcgt ttcggacgta acgctgcatc cattaccgct
    22381 tcaacttgcc cctcttcttc ctgcgcttca tcgagcgtgg ccggggcaaa attttgcgcc
    22441 tgattttcgt caaaggtctg ctgcaccctg aattttggat tctgatcgac ctccagtgga
    22501 ccgtcgaaat caatacgtgg ttttaacggt tcggtcatcg caatttatct cctattaaaa
    22561 attccagcgc agcatcaagg cgaatatgcg gcagcggttt gtcgacatcc atcacctgcg
    22621 gacgaaacgc ctcaaactgg aagccttgct tatcccagaa cgcctgacca ggcaaacgtg
    22681 cgggaacttc gccaggataa acagtgagcg gtgcgccatc actgagtcga ttaccgcgca
    22741 gcgccgggat tttctcaccg ttaacatcaa taatgccgct ggtggtcgcc tgaactgacg
    22801 ccagccccag gcagtccatg ctgatccctt cgaacgccgc attttgccag gcatcctgaa
    22861 ttagttgttg cagtaatgaa accatattgg cgtgctgatc gatggtcaca tggtctgctt
    22921 tagtcgcagc aaacaatagc ttatcgataa ccggcgaaaa caaacgccgg aacagggttc
    22981 gctgcccgta gtggaaactt tgcatcagct gcgtcagcgc cagacgcata tcattaaatg
    23041 cctgtggccc actgttgaga ggctgcaggc aatccaccag cacaatctgg cggtcaaagc
    23101 gcagaaaatg attcttatag aaccccttca ccaccttctc gcaataataa ttaaagcgct
    23161 cgcgcagcat tccggcattg gtatgcttat cggcctgcgc cagtttggac tcgccccagg
    23221 catcgacatc cggccacggg aagaattgca gcgcgggtgc tcctgccata tctcccggca
    23281 agacaaagcg cccaggctga ataaagtgca acccctgctg tttgcagtgg tggagatact
    23341 cggtccaggc ggcggcaatg tctgccagcc ggttttcgtc ggcaggcgct agcgggtcca
    23401 gcccttcgca catcattcgc catttcgccg accattcgcc gcgctgacca ttgagtaagc
    23461 ccgtcatctg gcgcgaccag cttaaatagt cctgcgccag catcggcagg tcgagcaacc
    23521 attcgccggg gtaatccaca atttccagat acagcgtgga ggtgtcctta aagtggcgta
    23581 gcagcgaatc gttcgattta aaacgtaacg ccaggcgaat ttcactgacg ccgcgcgtag
    23641 gcgtcggcca ggcgggagga tcgccatata actgcgccaa cccttcatca taggtgaagc
    23701 gcggaatgcc aaagtcacgc tggggaatgc gttttacgcc cagcaggcgc tcttcacgca
    23761 ccgcacttaa aagcggcaaa cgtgctccgg catgaatatt gagcaactga ttaaccatcg
    23821 cggtgataaa cgctgttttg ccgctgcggc taagtccggt tacagcgagg cgcagatgtc
    23881 tgtcgacacc ccgattcacc agcgcattaa gttcattttt aagtcgcttc attgctgtcc
    23941 tgtgccagaa agctgtagaa aggattagat actggagata ataatgtggg ttaattttga
    24001 tttatcaaat gagtaagcgg cgttgtttac accgctcaat aagtaatttg ttaacctttg
    24061 accttcggca ttgcgatgtc tttcaggccg ccggcgttct caacgtgggt atatcccatc
    24121 tcgctaagga tctcttttgc ctgccctgac tggcgtccgg cattgcaata cactttcacg
    24181 gtgtcgtttt tatccggaac ggcggtggca atgcgctctt tcacttcctt caggggaata
    24241 ttgatggctc cctgaacgtg ctcttgctga tactgctctg gaacacgaac atcgatccag
    24301 tgttcagcgg cgaaaacggg cagtgaaaac accagtgtca gagcaagtaa gcctttttta
    24361 aacatgtaag cgtccttcta tggatgaaaa ttgccatcca taatatgcct tagctttaac
    24421 aatttttgcg aaactcccct caccttttat aacgctgcgc cagtttattg gcagcccgac
    24481 ttaacagcgg ttccagcgcc acggccagca acattttcag tggtcggcga gcaactgatt
    24541 ttatcgccca gccagctaca cccgccgggc catagcgcag tgcggtaaga agtaccagct
    24601 tgcctgctaa tttgaaacca ggcttcacct tttgcccggc ctgttgccag cgagtattca
    24661 taactttcct cacagttgac ggaaacggct acgtaacgtg aaagtatcgg aagtgacata
    24721 acgttccatc tcgcgtaaac gcgtttcact tgccgccagt tcacggtcga cttcatccag
    24781 caattcactg ctggaaggta gctgctcacc aaaggccatg ttgtccggca ttggatcaag
    24841 cgcaaatgac aaaatgatgt aagcaaccag ggtaaacagc gccagaccga agaaaatcga
    24901 cagcaccacc aggatacgca ccagttttac tggtacatca aaatagttgg caatcccggc
    24961 gcagacgcca cggaccatgc cctgctgtgg aatacgccat aattttttat tgagattaat
    25021 gcccgccatt agcgatccct ccagttcgga tgttctgcat caagaatgga ttccagcgcc
    25081 tgaatacgtt cgcgcatccg ttttgcttca tcagccagtt gcgctaatcg ctgctgctca
    25141 ctttgcgaca attcactgcg accagaacga ttgctgtaat gcagccataa ccagatcggt
    25201 aaaacaaaca gcacaaaaat ggttaacgga atagccagaa atagcgcgct cataaggact
    25261 ccttagacga tgagcggcgg cgcgttcaga cgccgcctgg atgttattat tgattgtctt
    25321 gcttcatttt ggcttttaat tgtgccagtt gctcgctgat tgcatcatcg gctttcagtt
    25381 cggcaaactg atcgtccagc gatttttgtt taccgaagct gtggctttct gcttccgctt
    25441 ccatctggtc aattcgacgt tcgaaagatt cgaaacgagc cattgcttca tccagtttgc
    25501 cattgtccag ctgacgacgc acatcgcgcg acgagtttgc cgcctgatgg cgtaacatca
    25561 atgcctgctg gcgagcgcgt gtttcgctca atttgttttc cagctcgccg atctctttct
    25621 tcatgcgtgc cagcgtatcg tccaccagcg tcacttcatg ttccaggctc ttaatcagat
    25681 cggtcagttt ctgtttttca attaacgccg cacgcgccag atcctctctc tctttcagca
    25741 gcgccagttc ggctttttcc tgccattcaa cttcacgcgc cgacgcttgt tcaatacggc
    25801 gagtcagctg tttcttttct gccagcgcac gcgccgaagt agaacgcact tcaaccaatg
    25861 tatcttccat ctcctggatc atcaaacgca ccagtttctg tggatcttcc gctttctcta
    25921 acagagcgtt gatgttggca ttcacgatgt cggcaaagcg agaaaaaata cccataatgt
    25981 tgtcctcttg atttctgcgt tcaggattgt cctgctgaac tattaataca atttgcgtgc
    26041 caatttttta tctttttgat ttataaagat ctgattgaag aatcaacagc aacatgccag
    26101 gatgagttag cgaattacac taacaagtgg cgaatttcat catggcagaa tacaaagata
    26161 atttactcgg tgaggcgaac agctttctcg aagtgctgga acaggtttcg catctcgcac
    26221 cgctggacaa accggtgctc atcatcggcg agcgcggcac aggtaaagag ctgattgcca
    26281 gccgtctgca ttatctctcc tcccgttggc aggggccgtt tatttccctt aactgcgcgg
    26341 cgttaaatga aaatctgctg gattccgaac tgtttggtca cgaagcgggc gcgtttaccg
    26401 gtgcgcaaaa acgtcatcca gggagatttg aacgtgccga cggcggtacg ctatttcttg
    26461 atgaactcgc tacggcaccg atgatggtgc aggagaaatt attgcgcgtg attgagtacg
    26521 gtgaactgga gcgcgttggc ggcagccagc cattgcaggt gaatgtgcgg ttggtatgcg
    26581 cgacgaatgc cgatctcccg gcgatggtca atgaaggtac ttttcgcgct gacctgctcg
    26641 accggctggc tttcgatgta gtgcaactgc cgcccctgcg cgagcgcgaa agcgacatta
    26701 tgctgatggc agaacacttt gccattcaga tgtgtcggga aatcaagctg cctctgttcc
    26761 cggggtttac ggagcgcgcc agagaaacat tgctgaatta tcgctggccg ggaaatattc
    26821 gtgaattgaa aaacgtggtg gaacgttcag tatatcgcca cggcaccagc gattatccgc
    26881 ttgatgacat tattattgat ccctttaaac ggcgtccgcc tgaagacgct atcgccgttt
    26941 cagaaaccac ctcgcttcca acactgccgc tggatttacg tgagtttcag atgcagcagg
    27001 aaaaagagtt gctgcaactc agtttgcaac aagggaaata taaccagaag cgcgcagctg
    27061 aattgctggg gttaacctat catcagttcc gcgcgttgtt gaaaaagcac cagatttagc
    27121 gaagttgggc aatacaactt gccggatgcg gcgtgaatgc tttgtccggc atacataatc
    27181 aatacgtcgt ttgcaggcct gataagcgca gcgcatcaga cattattgcg ttgggctatt
    27241 aatcgttcag cgcatcctgc aatattttca ttgccgcatc gaattgcgca tccgggatgg
    27301 tcagcggata caggaaacga atcacgttgc cgtatgcgcc acaggtcagc agaagcagcc
    27361 cctgcgccag cgcgcgttgc tggattttct gtgcaatcgc cgctgacggc tcgcccgttt
    27421 gcggatcgtt aaactctgcc gcaatcatag accccagacc gcgtaccgcc gcgatggacg
    27481 gaacgctttc tttggcatca atcagcgtgt ttgtcagacg ctggcccagt tgattcgcgc
    27541 gttcgcagag tgattctttg tcgataatgt tgagcaccgc gtgcgcggca gccaccgcca
    27601 gcgggttccc ggcgtaggta ccaccaagcc cgcccggcgc gggtgcgtcc ataatgttgg
    27661 cattaccgac cacgcccgaa agcggcatcc cgcccgcgag gcttttcgcc atcgtcatta
    27721 aatccggctt atcggcgtag tgatccatgg caaacagttt accggtacgc gcaaagccgc
    27781 tttgcacttc atcggcaatc atcacaatgc cgtgatcgtc gcacaggcgg cgaatggcgg
    27841 caaccagctc ttttggtgca acgttgaaac cgccttcgcc ctgcaccggt tcgaaaataa
    27901 tcgccgccac ctgcttcgct tcaatgtccg atttaaacaa gcgttcgatg gcgtcgaggg
    27961 agtcctgcgt tgaaatgccg tgtaaatctg acggataagg tacgtgatat accgaaccgg
    28021 ggaacgggcc gaagccgatt ttgtacggcg cgacctttcc ggtcagcgcc atagtcatat
    28081 atgtacgacc gtggaagccg ccgctaaacg caatcacgcc agggcgtccg gtatgggcgc
    28141 gggcgatttt caccgcgttc tcaaccgctt ccgcaccggt ggtgaagaac gcagttttag
    28201 cctgcccgct caccggggca agggcgttaa ttttctccgc cagcgtgacg tagctttcgt
    28261 acggcacaat ctgatacgcc gtgtgggtaa actgttgcaa ttgctgctcc accgccgcga
    28321 ccagatcagg gtggcgatgc ccggtattca gcaccgcaat gcctgcggcg aaatcgatat
    28381 attcgttgcc ctctacatcc ttcagcgtag cgttttctgc cgactgggcg aagaagttac
    28441 acatcacgcc aaccccgcgt ggggtagcag aaagacgacg ctgatggaat tcattgttgc
    28501 tcatgtttca caccttttga ctcttcactg ttaatcggaa attaaacgcc cagacgatcg
    28561 cgcaggctgt aataagccgc gcccatcgcg gtaaacggaa cgcgcagcgt gcgcccgccg
    28621 gggaacgggt aatgcggcag attggcgaag gcgtcgaagc gttcggcgtc accgcgcagc
    28681 agttcagcaa tcaaacgccc ggcaagatgg gtacaggtga cgccgtggcc gctgtaaccc
    28741 tgcatgtaat agatgttggt atcaaggcga ccgaactgcg gcatgcgcga caaggtcagc
    28801 aggaagttgc ccgtccagcg gtaatcaatt ttcacgccct tcagctgcgg gaaggttttc
    28861 agcagtttcg gcaccacaag gcgctcaacg tcatccgggt cgcgcgcgcc gtagaccaca
    28921 ccgccgccgt acagcaggcg gttatcggcg gtaagacggt agtaatcaag cagatagtta
    28981 cagtcttcca cacagtagtt tttcgggatc agcgaacggg ctaaatcttc cgacagccgt
    29041 tcagtggtga tcacctgcgt gccgcacggc atgctgcgtt tcgccagttc cggctctact
    29101 ttatcgccca gatacgcatt cccggcgaca atcacatact tcgccgtcac ctgaccattg
    29161 gcagtccgca ccacggctgg cgtggtgtgc tggatctgcg tcacggcgga aagctcatac
    29221 acgcgccctc cgttcaggcg gatggcatcg gcttcaccaa tggcgaggtt tagcgggtga
    29281 atatgcccac cgctgtgatc cagaagcgca ccagtgtagc gatcgctggc gacctcgcgg
    29341 cgaatagcgt tggcgtccag cagctccagc tgtttattgc cgtagcgttc ccagttctct
    29401 ttctgctctt ccagagtcgc gagctgttta tcattcatcg ccacaaacag gccgccgggg
    29461 cggtagtcgc aatcaatctg ataacgtttg atacgttcgc ggatgatctc gccgccttcg
    29521 aacatcatgc tgccgagcat acgggcggtg tccatgccgt agcttttttc aatgacgtcg
    29581 atgtcgcggc tgtaggagtt cacaagctgc ccgccgttgc gtccgcttgc gccgaagccg
    29641 atgcgtgagg cttcaagaac caccacgtca aagcccgctt ccgccagatg cagcgcggag
    29701 gaaagcccgg tatagccgcc gccgaccacg caaacatcgc aggaaatcga ctcattcagc
    29761 gtgtcgaatg gtgcgtattt attcgcactg gcggcgtaat aactgctggt atgttcggtc
    29821 atttcaggcc tccaggctta tccagatagt tttcagttcg gtgaattttt caagggcgtg
    29881 cagggatttg tcgcgaccat tgccgctctg cttatagccg ccaaacggca cggtcatatc
    29941 gccgtcgttg tagttattga caaagacgga accggctttc aggcgacggc tcatgcggtg
    30001 cgcgcgggag aggtcgcgcg tccatactgc cgcgccaagt ccgtactggc tgtcgttagc
    30061 aagctgtagc gcctgttctt ctgatgtgaa acgcgtgacc accagcactg gaccgaaaat
    30121 ctcttcgcgg cttaaggacg cattcgggtc tacatccaca aagatcgtcg ggccgatggc
    30181 ggcagccagc tcggcgttac ggccatccag cagcagttgc cctttgcttt cgccttcacg
    30241 aataaagcta tggaccgagt cggcgtgggc gcagtcgatt aaggtgccca tggtggttgc
    30301 gggatcaagt ggatggcccg gctgccagtt ttgcgcctgc tgttttaaca ggtctaagaa
    30361 ttcatcggcg atgctctctt ccagcaacag gcgcgttccg gcgatacaca cctgcccctg
    30421 gttgtagaag atgcctgctg cggtcgcgct cgccgctttt tgcaaatccg gacagtcagc
    30481 gaaaacgatg ttggcgcttt tgccgcccgc ttccagccag acgcgtttca tgttgctgtc
    30541 gcctgcatct ttcagcagct gtttcccggt acgggtcgaa ccggtaaagg cgatggcatc
    30601 gatatcgtta tgacgcgaca gcgcctgccc cgcttcatga ccaaaacccg tcaccacgtt
    30661 caacacgcca tccggcagac ctgcttcttt cgccagcccg gcgagacgaa tcgcactgag
    30721 cggtgatttt tcagacggtt ttaggatcac gctgtttccc gccgccagcg ccgggccgag
    30781 tttccagcaa gtcagcagca gcgggaagtt ccacggcacg atggcggcaa tcacgccgac
    30841 cggttcacgc acgatcatcg ccagctcatg gctactggtg gtcgccactt cgccatacac
    30901 tttgtcgata gcctcggtgt accagcgaat ggcgcgcgcc gcgccgggaa tatcatcacg
    30961 caggctgtga cgaatcggtt tgccggtgtc gagcgtttcc agcagcgcca gctcttcggc
    31021 gttggcttcc ataaaatcgg cgagtttatt cagtaccgct ttacgtttag ccggagaaga
    31081 gagtgaccag tcgccgcgtt caaatacgcc gcgtgctgcg ctcaccgcac ggtcgatatc
    31141 gacgctcttg ccgcgggcaa ttttcgccag cggtgcctgg gtgaccggat caacggtttc
    31201 aaaagtttca ttttccgccg cagcagtata ttcaccgtta ataaataagc gggtttcaat
    31261 ggcgagactt aacgctttat cctgccagta agccagatga tgaaaattca ttatgactcc
    31321 tgtttcacgt ctatcagata tatgcagttt gtgaacgtca gggcgcagag atccccgggc
    31381 cgtatgcccg tttatttctg gcgacattct ttatagattg ccgggcgcat tgcgcttacc
    31441 cggcttacag aacaaaaaga ttaaaacgtg gtgggcgtat gggcgctgat aattcggcaa
    31501 atacctgccg acgtattact gaaactgtgc gggatgccgg tattaatggc ataactttgc
    31561 cccgcgacga ggtggtaatc ctgaccatta atcgtcagaa caatttcacc ttccagtaca
    31621 gtgcctattt cctcaccctg atgcttaatt ctttccccag tggttgtgcc cggctggtaa
    31681 gtttcaaaga tcatcgccag cgtgcgattc gggttaccgt tatgaaccag cttcattgac
    31741 acaccctgac tgcccatctc aattaagtcg tcctgattaa tgacgacctg cggctcatca
    31801 ggtttttccg gctcggaaaa gaattccgag agtgacagac catacacctt cagcagcttt
    31861 tgcagcgtac tgatggcagg gctgacttta tcttgttcta tcgtactgat agcactgtga
    31921 gtcagcccgg agagttcggc ggcacgacgt tgtgaaagcc cctgctgctg gcggatttcc
    31981 gacaagcgtt ttcctggcgc cagtccctca tcactcatat ttgcatttcc ttaaactgta
    32041 gtggtcagag tcgctgtttt tcagcgatat ggtgctgaca agcggtgata aagccctcga
    32101 acaatatacg cgaaagcgcg tactcgctac tgttccattc cgggtgccac tgtacgccca
    32161 gcgcaaaagg atgattgatg acgctaaccg cctcaactaa gccatccggc gagcgagctt
    32221 caacacgcaa ccgtgggcta acgaccttcg ccccttgtcc atgcagagag tttacccaaa
    32281 agttgctaca ttcaggtaac aacgcagaca gtaatccccc ctcttcaacc agaacttcat
    32341 gcgacggcgc atattgctgc tccaccggca gttcgggatc ttcccgatgt tccagcaatt
    32401 caggctgttc gcacagcttg cgatgcaacg aaccaccggt tgccaccacc agttcctgta
    32461 aaccccggca gatggcgaaa atggggatgc gcctttcgag tgcggcatta attaacgcca
    32521 tgctcagaag atcacgcccg ggatcggcgt caggctcatc gccgttttca ccatataggt
    32581 gcggctgcac attgctggga ctaccaggaa gataaatacc atcgagtttc ggcaaaagtt
    32641 gttcaagtaa tgacggttcc gccagcgcat gtggcagcgc aattggcaag ccgcctgcgt
    32701 ggatgatggc attcaggtac ttttcttgca gagtctgggt cgcatgaccc ttaagcctgt
    32761 tcctgcacat tacgacaccg ataaccggat tgttcattat attttccatg ctcaatctca
    32821 caaagtggac taaattatcg ccattactgc gctttaaatg acctttatgt tcaatatttt
    32881 ttcaatctag cagtgggttt tcatttttgc aaactcaatt taacatttga caaacattta
    32941 gtttgcatac agattcgaat ggtggtcatt atattttacg ctttgataac gagcggaaaa
    33001 caaaccaaag gcgaagaatc atggaaacca atatcgttga agtagagaac tttgttcagc
    33061 agtcagaaga gaggcggggt agcgccttta cgcaggaagt gaaacgctac ctggagcgct
    33121 acccgaatac acaatatgtt gatgttttac ttaccgattt gaatggttgc ttccgtggaa
    33181 aacgtattcc tgtttcaagc cttaagaagc tcgagaaagg gtgttatttc ccggcctcgg
    33241 tatttgcaat ggatattctg ggtaatgtag tagaagaagc gggcctgggt caggaaatgg
    33301 gcgagccgga tcgtacctgt gttcctgttc tcggttcctt aaccccttct gccgccgatc
    33361 cagagtttat aggtcagatg ctcctgacca tggtcgatga agatggcgct ccctttgacg
    33421 ttgagccgcg gaacgttctt aaccgcctgt ggcagcagct gcgccagcgc ggattgttcc
    33481 cggtcgtagc ggtagagctg gagttctatt tactggatcg ccagcgcgac gctgaagggt
    33541 atctgcaacc gccctgcgcg cccggcaccg atgaccgcaa tacacaaagc caggtttact
    33601 ccgttgataa cctcaaccac ttcgccgacg tgctcaatga tattgatgag ctggcgcagt
    33661 tacagctgat tccggcagat ggcgcggtcg ccgaggcctc gcaaggtcag tttgaaatca
    33721 atctttacca taccgataac gtgctggaag cttgcgatga tgcgctggca ctaaaacgcc
    33781 tggtgcgtct gatggcagaa aagcataaga tgcacgccac ttttatggcg aagccgtatg
    33841 aagagcacgc gggcagcggg atgcatatcc atatcagtat gcaaaataac cgtggcgaga
    33901 acgtgctttc tgacgcggaa ggcgaagatt cgccgctgct gaaaaagatg ctcgccggga
    33961 tgattgacct gatgccgtca tcaatggcgt tgctggcacc aaacgtgaac tcgtatcgcc
    34021 gcttccagcc gggaatgtat gtgccgacgc aggcgtcgtg gggccataac aaccgcaccg
    34081 tcgccctgcg tattccctgc ggcgatcgcc ataatcaccg cgtggaatat cgcgtggcgg
    34141 gtgccgatgc caacccgtat ctggtgatgg cggcgatttt tgccggtatt ttgcatggcc
    34201 ttgataacga actgccatta caggaagaag tcgaaggcaa cgggctggaa caggaaggct
    34261 tacccttccc gattcgccag agcgatgccc tgggtgagtt tatcgagaat gatcacctgc
    34321 gccgctattt aggcgaacgc ttctgccatg tctatcacgc ctgtaaaaat gatgagctgt
    34381 tgcagtttga gcgcctcatc acagaaacag aaattgagtg gatgttgaaa aacgcgtaat
    34441 accccttgcg gggttgtttc atcggcctga gcggagccag gcctgacatt tgctggtaat
    34501 ccagacattt cccggatgtc gcgttatgga gcgcaggcgg caacgggctt ccgttaacga
    34561 ttaagactgt gacgaggaaa tgagatgatg ccgatgatta aatccccgca tggcgaggga
    34621 ggctgcgtat gcgcccctcc tgcgactgac tggactccgc cccctttgct tcccttgcta
    34681 aacaggtttg atttcaggtc gacacgaccg caaaccttat tacgcagggg aggcagcaac
    34741 tatggctatt aattcaccac tgactattgc tgcgcaatct ggcaaaaccc gtctgcgaaa
    34801 atcactgaaa ttgtggcagg tggtgatgat gggtctggcc tatctcacgc cgatgaccgt
    34861 atttgatact tttggtattg tctccggcat tagcgacggt cacgttccgg cgtcctattt
    34921 gctggcgctg gcgggcgtgc tgtttaccgc tatcagctac ggcaaactgg ttcgccagtt
    34981 tccggaggcc ggttcggcct atacctacgc gcaaaagtcg attaacccgc acgtcggatt
    35041 tatggtcggc tggtcatcac tgctggatta tctctttttg ccaatgatca acgttttgtt
    35101 ggcgaaaatc tatctctccg ccctcttccc ggaagtgccg ccgtgggtgt gggtggtaac
    35161 cttcgtcgcc attttaaccg ccgcgaatct gaagagcgtc aacctggtcg ctaacttcaa
    35221 taccctgttt gtactggtgc aaatctccat catggtggtg tttatcttcc tggtggttca
    35281 gggactgcat aaaggagaag gcgttggcac cgtctggtca cttcagccgt ttatcagcga
    35341 gaacgcgcac ctgatcccga ttattaccgg ggcgacgatt gtctgtttct ccttcctcgg
    35401 tttcgatgcg gtgaccacgc tttcggaaga gacgccggac gccgcacgcg tgatcccgaa
    35461 agccatcttc ctgacggcgg tatacggcgg cgttatcttt atcgctgcgt cgttctttat
    35521 gcagctgttc tttcccgata tcagccgctt taaagacccg gacgccgcac tgcctgaaat
    35581 tgcgctatac gtcggcggca agctcttcca gtcgattttc ctctgtacca cgtttgtgaa
    35641 cacgttagcg tctggcctgg cgtcgcacgc cagcgtgtcg cgtctgctgt atgtgatggg
    35701 gcgtgacaat gtgtttccgg agcgcgtgtt tggctatgtg cacccgaaat ggcggactcc
    35761 ggcactgaac gtcattatgg tcgggattgt cgcgttgtca gcgctgttct ttgatttagt
    35821 caccgcgaca gcattgatta acttcggtgc tctggtggca tttaccttcg taaatctgtc
    35881 ggtgtttaat catttctggc ggcgtaaagg aatgaataaa agctggaagg atcacttcca
    35941 ctatttgctg atgccgctgg ttggcgcgct gacggtgggt gtgctgtggg ttaatctcga
    36001 gtcaacgtca ctgacactcg gtctggtatg ggcttcgtta ggcggcgcat atttgtggta
    36061 tctgatccgc cgctatcgca aagtgccgtt gtatgatggt gacagagcgc cggtgagcga
    36121 aacgtaatag tcggatgcgc ggcgcattat cctgcctgag ggtaatgcac cgcgccatta
    36181 actataaaaa gatgtatgac ccggatcacg agtgctatca tccgccctta tgcttagcgc
    36241 attaacccca gggggaaata atgaaccacg atatcccgct aaaatatttt gatattgccg
    36301 atgagtacgc gaccgaatgc gcagagcctg tcgcagacgc tgaacgcaca ccgctggccc
    36361 tctacttcca gttactgctc acccgtttga tgaacaatga agagatcagc gaagaagccc
    36421 aacatgaaat ggctgctgaa gcggggatta atcccgtacg cattgatgag atcgcagagt
    36481 ttttgaatca atggggtaat gagtaattat ttcattgcag acgctcggtc agtatcgttg
    36541 cccggataaa gcgttcacgc cgcatccggc taacaatgac gcgcactgca gcgtgcttta
    36601 atgggtttct cacgatcgta ccgctctgct cctcgctaag ttgttctgtg agttatagtt
    36661 atagagaaat cacgggtacg aatgttgaac agatggcgac gtcacctgtt caacgaccaa
    36721 ccacacggcc ctcattacga cccctgaaca caaacggtaa tggcaaatgt cattaaatat
    36781 tagtaagata gcctctttac ataaaatcca ttaatattat gcgccaggta ttatcgtctc
    36841 ttttggtgat tgctggactt gtgagtggtc aggcaatcgc cgcgcctgaa tcccccccgc
    36901 atgctgatat ccgcgatagc ggttttgtct attgcgtcag cgggcaagtc aacaccttta
    36961 acccatccaa agcgagcagt gggttaattg tcgataccct tgccgcccag ttttatgatc
    37021 gactgctgga tgtcgatccc tatacctatc gcctgatgcc ggaacttgcc gaaagctggg
    37081 aagttctcga caacggcgcg acctatcgct tccacctgcg tcgcgatgtt ccgtttcaaa
    37141 aaaccgactg gtttactccc actcgtaaaa tgaatgccga cgatgtggtg tttaccttcc
    37201 agcgaatttt tgaccgcaac aacccgtggc ataacgtcaa cggcagcaac ttcccctact
    37261 tcgacagcct gcaatttgcc gataacgtga aaagcgtccg caaactggat aatcatacgg
    37321 ttgagttccg actcgcccag ccggatgctt cttttttgtg gcacctggcg acccattatg
    37381 catcggtcat gtcggcagaa tatgcccgga agttagagaa agaagatcgt caggaacaac
    37441 tcgaccgtca gccggtcggc actggaccgt atcagttgtc ggaataccgc gccgggcaat
    37501 atattcgctt acaacgtcat gatgatttct ggcgtggtaa gccgttgatg ccacaagtgg
    37561 tggtggattt aggctctggc ggtaccggac gtctgtcgaa actcctgacc ggggagtgcg
    37621 acgttctggc ctggcctgct gccagtcagc tatccatttt gcgtgacgac ccacgcttgc
    37681 gtttaacgct gcgtcctggg atgaacgtcg cctatctggc attcaacacc gccaaaccgc
    37741 cgctaaataa tcccgctgtc cgccatgcgc tggcactggc gattaataac cagcgtctga
    37801 tgcaatccat ctattatggt acggctgaaa cggcagcctc tattttaccg cgcgcctcgt
    37861 gggcctatga caacgaggct aaaattactg aatataatcc ggcgaaatcg cgcgaacagt
    37921 tgaaggcgtt ggggctggaa aacttaacgc tgaaactgtg ggtaccaaca cgttcgcagg
    37981 cgtggaaccc cagtccactg aaaactgccg aattgattca ggcggatatg gcacaggttg
    38041 gcgtaaaagt ggtgattgtg ccggtagaag gtcgctttca ggaggcgcgg ttgatggata
    38101 tgagccatga tctgacgtta tccggttggg cgactgacag taacgacccg gacagtttct
    38161 tccgtccgtt actgagctgc gcggcaattc attcgcagac caacctcgcc cactggtgcg
    38221 atccgaaatt cgacagcgtg ttgcgtaagg cgctctcctc gcagcagctg gcggcgcgta
    38281 ttgaagccta tgacgaagcg cagagtattc tggcgcagga attacccatt ctgccgctgg
    38341 cgtcgtcatt gcgcttgcag gcctatcggt atgatatcaa aggtctggta cttagtccgt
    38401 ttggtaacgc ctcctttgct ggggtgtatc gcgagaaacc ggatgaggtg aaaaaaccat
    38461 gattatcttc accttacgcc gcattttgtt attgattgtc accttgttcc tgctgacctt
    38521 cgttggcttc agcttgagct atttcactcc acacgcgccg ttacaaggcg cgtcactgtg
    38581 gaatgcctgg gtgttctggt ttaatggcct gatccactgg gattttggtg tttccagcat
    38641 taacggtcaa ccgattgccg aacagttaaa agaggtcttc ccggcgacga tggaactgtg
    38701 catcctcgcc ttcggttttg cgctgattgt tggcatcccc gtggggatga ttgccgggat
    38761 tacgcgccat aagtggcagg ataacttgat caacgccatc gccttactgg gcttttcaat
    38821 cccggtgttc tggctggcgc ttctgttgac gctgttttgt tcactcacgc taggttggtt
    38881 gcccgtttca gggcgtttcg atctgctcta cgaagtgaaa ccgattaccg attttgcgtt
    38941 gattgatgcc tggctttcgg actcaccatg gcgggatgaa atggtcatga gcgcaatccg
    39001 ccatatgata ttgcccgtga tcaccctgtc ggttgcgcca acaactgaag tgatccgact
    39061 gatgcgtatc agcactatcg aagtgtatga ccagaactat gttaaagcag cggcgacccg
    39121 cggtttgtcg cgctttacta tcttgcgtcg ccacgttctg cataacgcgt tacctccggt
    39181 tattcctcgc ctgggcttac agttttctac catgctgacg ctggcgatga tcaccgaaat
    39241 ggtttttagc tggccgggtt tagggcgctg gttaattaac gccatccgcc agcaggatta
    39301 cgcagccatt tccgccggag tgatggtgtg tggctcactg gtcattattg ttaacgtgat
    39361 ttctgatatt ttgggtgcca tggctaaccc tctgaaacat aaggaatggt atgccttacg
    39421 atagcgtata cagcgaaaag cgcccgccgg gcacgctgcg taccgcctgg cgcaaatttt
    39481 atagtgacgc ctctgcgatg gtcggcctgt acggctgcgc ggggctggct gtactgtgta
    39541 tttttggcgg ctggtttgcg ccttacggca tcgatcagca atttctcggt tatcaattat
    39601 tgccgccgtc atggtcgcgc tatggcgaag tttctttctt ccttgggact gacgacctgg
    39661 gacgcgatgt tttaagccgt ttactgagcg gagctgcgcc aaccgtaggc ggcgcatttg
    39721 tggtgacgct tgccgcgacg atctgcggcc tgatactggg caccttcgcc ggcgcgacgc
    39781 acggcctacg ctcagcggtg cttaaccata ttctggatac cttactggcg atcccttccc
    39841 tactgctggc aattatcgtt gtagcgtttg ccggaccgag tttgtctcac gccatgtttg
    39901 ccgtctggct ggcgctgctg cctcgtatgg tacgttcgat ttacagcatg gtgcatgacg
    39961 aactggaaaa agagtacgtt atcgctgccc gtctggatgg cgcatcaacg ctgaatattc
    40021 tctggtttgc tgtgatgcca aacatcaccg ccgggctggt caccgagatc acccgcgcac
    40081 tgtcgatggc aattctcgat atcgccgcgc tgggctttct cgatctcggc gcacaactcc
    40141 cctcgcctga atggggagcg atgctcggtg atgcgctgga actgatttat gtcgccccgt
    40201 ggactgtcat gctgccaggt gcggcaatta tgattagcgt gctgttggtt aacctccttg
    40261 gcgacggtgt ccgtcgtgca attattgcgg gggtggaata atgccattac tcgatattcg
    40321 taacctgacc attgaattta aaaccggtga tgagtgggtt aaagccgtcg accgcgtaag
    40381 tatgacctta accgaaggtg aaatccgcgg tcttgttggc gaatccggtt ccggcaaaag
    40441 tttgattgcg aaagcaattt gtggggtgaa taaagataac tggcgtgtta cagctgatcg
    40501 tatgcgtttt gatgatatcg atttgctgcg tctctccgca cgcgaacggc gcaagctggt
    40561 tggtcacaac gtgtcgatga ttttccagga accgcagtcg tgtcttgatc cttcagaacg
    40621 agtgggtcgc cagttgatgc aaaacatccc agcctggacc tataaaggcc gttggtggca
    40681 gcgttttggc tggcgcaaac gccgtgccat tgaactgctg caccgcgtgg ggattaaaga
    40741 tcacaaagat gcgatgcgca gttttcccta tgagttgacc gaaggcgaat gtcagaaagt
    40801 gatgatagcc attgcactgg cgaatcaacc gcgtctgctg attgctgacg aaccgacaaa
    40861 ctcaatggag ccaacaaccc aggcgcaaat ctttcgcctc ctgacgcgtc tcaaccaaaa
    40921 cagcaatacc actattttgc ttatcagcca tgacttacaa atgcttagcc aatgggcgga
    40981 taaaattaac gtgctttact gcggtcaaac tgtggaaacc gcgccaagta aggagttggt
    41041 gacgatgcca catcatcctt atacccaggc gctgatccgc gcgataccag acttcggcag
    41101 cgcgatgccg cataaaagtc gcctcaatac gctgcccggc gctatcccac tgctggaaca
    41161 gttaccgatt gggtgtcgtc tggggccgcg ttgcccgtat gcacaacgag aatgcattgt
    41221 gacgccacgt ttgacggggg cgaaaaatca tctctatgcc tgtcatttcc cgctgaacat
    41281 ggagaaagag tgagatgatc gaaacgctgc ttgaagtgcg taatttaagt aaaacgttcc
    41341 gctaccggac cggttggttt cgtcgtcaga ccgtagaagc ggtaaaaccc ttgagcttta
    41401 cgctacgtga aggccagaca ctggcgatta ttggcgagaa tggttcgggt aaatccacgc
    41461 tggcaaaaat gctggcggga atgatagaac ctaccagcgg cgagttattg attgacgatc
    41521 atccgctgca ttttggcgat tattccttcc gcagtcagcg tattcgcatg atttttcagg
    41581 atccttcgac ctcgttgaat ccccgtcaac gtatctcgca aatcctggat tttccactgc
    41641 gtctgaacac cgacctggaa ccggaacagc gtcgcaaaca gattattgaa acgatgcgta
    41701 tggttgggct gctaccggat cacgtcagtt actatccgca tatgttggca cccgggcaaa
    41761 aacagcgtct ggggctggcg cgcgcgctga tattgcgccc aaaagtcatt attgccgatg
    41821 aagccctcgc ctcactggat atgtcgatgc gttcgcagtt gattaatctg atgcttgagt
    41881 tacaggaaaa acagggcatt tcgtatattt atgttaccca gcatatcgga atgatgaagc
    41941 acattagcga ccaggtgctg gtgatgcatc agggcgaggt tgtcgagcga ggcagcaccg
    42001 ccgatgtgct ggcatcgccg ctgcatgaac tcaccaaacg gctgatcgcc ggtcattttg
    42061 gcgaggcatt gacggcggat gcgtggcgta aggaccgtta gtgttctgca cctcatcctg
    42121 acagggcccc cctcaggatg aggtgttcat tacttttgat tgtcgattct ggtgagagca
    42181 accagcaatg ccagcgtata acacaccgta attgccagac tcatttttaa cagtgtttct
    42241 ccgccaaacc ccgacaatgg cgcggctata ccgccgaaga caaacatgct catccccatc
    42301 aacgcagacg ccgttccaga ttcctgcgtc cctactccac tcatagcctc tgccccgcca
    42361 acagtgccga tgccgctaca aaatgcaata gtgaaaaata atgccaccag tgccggaatg
    42421 ggcaattgtg tccatgcgca taagaccgtg agcaatgcac acaaaattgc cgctatcagg
    42481 ccacctcgca acaatgtcat cgcgttaatc cgtcgcgcca ggcgcgagaa gatccatgaa
    42541 gcaatgataa gtccgatgcc gttaaggcca aacaccaggc taaattgcat tggactaaag
    42601 ccaaattcct tctgcaacac gaacgacgaa gagccgatat atgcaaacaa accggccagc
    42661 ataaaacttt gaatcaggca aaagcgcata aaacggcggt tctgcaccac gcttcgtccg
    42721 gttaatagca atgatgagcc ccttttattt tctggcaagg tctcattaat aaataacagg
    42781 cagcccagca acagtacggt gctaatttca gccattaccc agaataaagt gcgccagtca
    42841 aaagtgctga caatgtaccc gcccagcact ggcgacaaca ccggtgccag gccattcacc
    42901 gtcattaaca gcgcaaaaaa ctgggtcaac gttactccct gatatttgtc acgagcaata
    42961 gaacgagaga gcaccgaacc acccgccccc gcgatccctt gaataaaacg ccagaccacc
    43021 agccagtaaa tattgttcgt tgtcgcgcac aaaatggaag aaacaataaa caatagcaac
    43081 gacaagatta acggacgttt acgccctatt ttgtcgctca gagggccaaa taacagttgt
    43141 ccgacgccaa gaccgatcag cgatgcggtc agggttaatt gcgttatggt cgtggttgcc
    43201 gccagttgct cagacatctc cggcaaagcc ggcaaataaa gatcggtaca catcgggccg
    43261 ataccggcta aaagaccaag aatcaatgcc catgaaagag agactctagc catattattc
    43321 cttacttgag tgatgacatg ccgccgccca gcgactgcca gagcgtgatt cggtttgtaa
    43381 aatcagtcag ttgtagtgcc agcagcgcct gctgtgcgct ccacaagctg cgctgcgccg
    43441 tcaacacggt caggtaatca ccaacacccg cctgatagcg acgcaagcca acatcgaccg
    43501 tttgttgctc agctttaaca tactggcgct gtgcatccag ttgctcttca agcgtggtcc
    43561 gccgcgccag tgcgttagcg acatctttaa atgcgctctg aacggttttt tcataggtgg
    43621 caatgagtcc acgcttttgt gcttctgcgt aacgaagctg cgccagatta cttccgccag
    43681 tgaacagcgg taatgtgacc gatggcgtaa atgaccagat ctgcattccg tggctgaaca
    43741 acgacgacaa tgcttcactg ccaacaccag cgctggcagt cagcgaaatc gtcggaaaaa
    43801 agttagcccg cgccgcgcca atatcggcat tggcgctctt caaattatgt tccgcttcct
    43861 ggatatccgg gcgacgcagc agaacgtcag atgacactcc cgcaggaacc agacttatca
    43921 tctgttccgg caggctttcc agtgttcccg gtagcaaatt ttcctcaagc gttgtacctg
    43981 ccagtaagtt cagcgcattt ttatcctgca tcacgtgtgt ctgatagctg gcaacgctgg
    44041 cgcgcgcttg ctgataaaca ctcatcgcct cactgacatc tgtcgctgcc gccgtgccaa
    44101 cctgttgctg gcgctggata attttcaatg agttttctgc actggccatc gtttcttttg
    44161 ccagggccag gttgctgtta tcagccgcca gtgtcagcca ggcggtgctg atttcagcaa
    44221 tcagcgttaa acgagtgttc tgggctgtaa attcactggc aagccaggtt tcccgcgccg
    44281 cccgagataa actctgatta cgaccaaaca gatctaacgt aaaactggac accgtgccgt
    44341 cagcctcggc agtggtttcc gtaccattcg ccagcgaacg actgcgggtt gaacttaagg
    44401 ccgcattcac cgtcgggaat aacgacgcat tagtttgccc atagagcgcc cgtgctgagt
    44461 caatatcagc aatcgctttt tgcacatcgc ggttactgtt cagtgcaata gtcaccacct
    44521 gctgcaatcg gggatcgtgg atcacctgct gccagtcatg gctgattgcc tttccctggc
    44581 cctgtgctcc cggcaaggtc gccgggattg gggattcagg cgtgctgtag tggggctcga
    44641 gcgagacaca tcccgcactc aacagcacca gaaaaataag agaacgacgc aacataactt
    44701 actcctgacg gcgcggttta ccggcaaaca aacgtttcac cagtacaaaa aacagaggaa
    44761 caaagaaaat agccagcaac gtagcggtca gcgtaccgcc aataatgccc gtaccaatgg
    44821 cgatgcggct gttcgcccct gcgccggttg ctatcgccag cggcattacc cccgcaataa
    44881 acgccagcga ggtcatgatg attgggcgta aacgagtctg cgccgcgcgt aatgccgcac
    44941 gactcaagga atagccctct gcaaccgccg cttcagcaaa ttccacaatc agaatggcgt
    45001 ttttcgacga caagccgata gtggttaata gcgccacctg gaagtaaaca tcgttgttta
    45061 aatcacgcat ccaggccgcc agcgccgcgc caagaagccc cagagggatc accagaatga
    45121 ccgagaacgg gactgaccag ctttcgtaca gtgctgcaag gcagaggaaa accaccagaa
    45181 tagaaactgc atacagactc aatgcctgac cgctggccag tttctcctgc aatgacaaac
    45241 cactccaggc ccaggttgtt cccgcaggaa gactgtttgc cagttcctcc atttttgtca
    45301 ttgcatcgcc ggaactaaag ccagtcgcat tttccccctg aatttcatag gctgcagaac
    45361 cgttgtagcg caccagtctt tccggtccat acagccagcg ggtggttgca aaagcagaga
    45421 atggtgtcat ggcgttatcg ctaccgcgca caaaccattt acctaagtct gacggcgtgg
    45481 aacgaaattc actgtcgccc tgaatgtaga cttttttcac acgaccacgg tcaataaagt
    45541 cattcacata agtaccgccc cacgcactgg acagggtgtc ggtgacatca ttcaaactca
    45601 accctaatga caccgcttta ttactatcaa tatctacctg caattgcggc atttgtggca
    45661 aatcattggc acgcacagaa tgtaactccg gactttgatt cgctttttct atcaactgat
    45721 tacgcatttg cagtagtgtt tcgcgatcgg ttccaccgtt agctaacaat tcaaacgtaa
    45781 aaccattgct ttgccccagc ccatcaacgg ctggcggcgt catcgcaaat accgtggcat
    45841 cacgaattgt gcccagctct ttggttgccc gtagggcgat agcctgggcg gtgttttctg
    45901 cccctttacg ttgagaccag tttttcaacg aaacaaacgc catcccggtg ttctgtccgc
    45961 tgccgctaaa actgaaacca tcaacggtaa aaatgacatc ggtatttgct ttctcattaa
    46021 tcaggaacca gtcaacaatc tggcgattca cttctgctgt acgggcagcg gtcgccccgg
    46081 ccggcagcgt gtactgcacc atgatttcgc cctggtcttc agtgggtaag aaactgcccg
    46141 gcagtttcca catcatcagg gccattccac cacccagtac gacataaagc cccatcgttc
    46201 gagcagcacg gcgcaggaca taaattacgc ctcgctgata tttatcttca gtacggcggt
    46261 aaaagcggtt aaatgcgccg aaaaagcctt ttttatgtgg cggaacatgc tggaggacgg
    46321 aaccgcacag ggcgggagtc aaggtcaatg ccactaccac ggaaagcagc attgcggaga
    46381 taatggttat cgaaaactga cgataaatta cccccgtaga tcccccaaag aaggccatag
    46441 gcaggaatac ggctgacaac accagcgcaa tggcaaccag tgcgccagaa atctcgccca
    46501 ttgatttttc cgtggcttca cgcgcgggca acccttcgtc acgcataata cgttcgacgt
    46561 tctccaccac cacaatggca tcgtccacca gcaagccgat cgccagcacc attgcaaaca
    46621 acgtcagggt attaatcgaa tagccaaaca acgcaagaac gccgaatgtg cccagcaaaa
    46681 cgacggggac agctaacgcc ggaatcagtg tggcacgcag gttttgcagg aataaataca
    46741 tcacgcaaac caccaatacg atggcttcaa acagcgtctg aattacgtcc tctacagaga
    46801 ttttaataaa ttcagtgctg tctttcgggt atgcgatgtc gtatccctgc ggcatgttcc
    46861 gctggaattc ggcaatctta tccttgacca gcgtcgccgt attcagcgca ttcgcaccgg
    46921 gcgaaagcat tacagcaacc ccggcagacg ggtgaccgtt aagtttgccg atagcggtat
    46981 aatcttcact gcccatctca acgcgagcca catcttttat acgaacaact gcgccgtctg
    47041 actggctttt cacgataata tttttgaact gatccaccgt ctgcaagcga gactgcgcac
    47101 gtaccgttgc ggtcagttgc tgagtattcg gtgaaggcaa tgcccctatt ttcccggcag
    47161 taacctgcac gttttgcgct tcaatagcac tttgcacgtc tgaaggcatt agcgagtaag
    47221 acgccagttt ggccgggtca agccagatgc gcatagcgta ttccgcgcca aagacttgca
    47281 gactccccac accttcaaca cgcgccagcg gatcctgaac gttactaacc aaccagtcgg
    47341 cgatatccga actggaggct ttgtcagtgg tgtcatacac ggcagcaatc agcaaaaagt
    47401 tgctttgtga tttctccacc gtaacacccg tttgctgcac ttcgctgggt agacgcgact
    47461 ccgcctgctg aattttattc tgcacctgaa cctgtgcagt atctggatcg gtaccttgtt
    47521 caaaggtcac attaatactg accgaaccat cagagctgct ggttgagctg aaatagagta
    47581 aattatcaag cccagtgagt tgttgctcga taacctgagt cacgctgttc tccagcgttt
    47641 cggcagaagc accagtataa gtggctgaaa ttttaatggt cggtggcgca acgtcaggat
    47701 attgcgcgac gggcaatgtg cgaatagcca gaatccccgc caacataatc aaaatggcga
    47761 taacccaggc aaagaccggg cggcgaacaa aaaagcgcga aaacatcagg cgttgcctcc
    47821 attagcctga acttcaacag ccttgacggt ctgccctgaa gtgactttgg cagaaccttc
    47881 aacaatcagt cggtcgccgt tgtgcaggcc gttcagcacc agccatttat caccatacgt
    47941 ttctcccgtt tcgagcgttc gctgctctac tttattgtct ttattcacca ccagcgcagt
    48001 tgcattgcct ttagcatcgc gcgtgacgcc ctgttgcggc gcgagaatag cgtcttccat
    48061 cacgccttca tcgacacgag cgcgaacaaa cattcccggt aataactgct gttgtggatt
    48121 ggggaaaatc gcccgtaatg tcaccgaacc ggtagactca tctacagcga cttcggtgag
    48181 ttccagacgc cctttttcgc tgtaggttgt gccatcttcc agaataagtg agacgctcat
    48241 ggtgtcactg ttggtcgcca gtgactgttt acgcagacgt aataaatcga cactggagcg
    48301 agtgaggtcg acatacattg tatccagacc acgaacagtc gttaacgctg tatcttgcga
    48361 cgcggtcacc agtgcgccag gggttaccga cgaaatgcca atgcgccctg aaatcggtgc
    48421 ggttaccgtg gtccagtcaa gattaatgcg cgcagtttcc agtgcggctt ttttcgcctc
    48481 tacactggct ttatcttgtg cacaggtaga ctgagcatca tcagcatcct gttgtgaaac
    48541 accgttctct ttcaccagtc gggcataacg ctgcgctttc tggcaatcgg cttttaccag
    48601 tgcctgtgct tgttgtaatg ctgcccgagc ttcattccat gcagcctgat aactggccgc
    48661 atcaatctgg tagagcggct gtccagcctt gaccagatca ccttccttaa ataagcgttt
    48721 ctggataatt cccccaacct gcggacgtac ttcagcactg agcgcagcac tggtgcgtcc
    48781 ggttaattca ctgactaccg aaaccggttg agatttgact gtaacaacgc cgacttctgt
    48841 ttctgacggg gatgaattgt tactttgcgt attatcacaa cccgataaga gcagcattgc
    48901 cactacagat gttgctatat acttcattat tttctccagg gtgagaaata ccctcctcat
    48961 taaaattaat gaggatatgt cagggatatt cagttataac gaacccgcat cgcgaaatga
    49021 atttcgcaat gaaataaggg ggattaatat cccccgcta
//
LOCUS       DACBVI010000016        48588 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.016, whole genome shotgun sequence.
ACCESSION   DACBVI010000016 DACBVI010000000
VERSION     DACBVI010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.016"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            448..1665
                     /locus_tag="IU325_04730"
     CDS             448..1665
                     /locus_tag="IU325_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP5808345.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1749..2348
                     /locus_tag="IU325_04735"
     CDS             1749..2348
                     /locus_tag="IU325_04735"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HAP5808346.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2407..4239)
                     /locus_tag="IU325_04740"
     CDS             complement(2407..4239)
                     /locus_tag="IU325_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAP5808347.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGEAMEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4326..4976)
                     /gene="hxpA"
                     /locus_tag="IU325_04745"
     CDS             complement(4326..4976)
                     /gene="hxpA"
                     /locus_tag="IU325_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HpxA"
                     /protein_id="HAP5808348.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTCLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKVAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4987..5481)
                     /locus_tag="IU325_04750"
     CDS             complement(4987..5481)
                     /locus_tag="IU325_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAP5808349.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5564..6019)
                     /gene="yfbV"
                     /locus_tag="IU325_04755"
     CDS             complement(5564..6019)
                     /gene="yfbV"
                     /locus_tag="IU325_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HAP5808350.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6357..7559
                     /locus_tag="IU325_04760"
     CDS             6357..7559
                     /locus_tag="IU325_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP5808351.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7634..9778
                     /locus_tag="IU325_04765"
     CDS             7634..9778
                     /locus_tag="IU325_04765"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP5808352.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9968..11488
                     /gene="yfcC"
                     /locus_tag="IU325_04770"
     CDS             9968..11488
                     /gene="yfcC"
                     /locus_tag="IU325_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP5808353.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11521..12063)
                     /gene="yfcD"
                     /locus_tag="IU325_04775"
     CDS             complement(11521..12063)
                     /gene="yfcD"
                     /locus_tag="IU325_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HAP5808354.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12121..12672)
                     /locus_tag="IU325_04780"
     CDS             complement(12121..12672)
                     /locus_tag="IU325_04780"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HAP5808355.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12728..13372)
                     /locus_tag="IU325_04785"
     CDS             complement(12728..13372)
                     /locus_tag="IU325_04785"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HAP5808356.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13508..14155
                     /gene="yfcG"
                     /locus_tag="IU325_04790"
     CDS             13508..14155
                     /gene="yfcG"
                     /locus_tag="IU325_04790"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HAP5808357.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     HISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14212..14574
                     /gene="folX"
                     /locus_tag="IU325_04795"
     CDS             14212..14574
                     /gene="folX"
                     /locus_tag="IU325_04795"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HAP5808358.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14595..15488
                     /locus_tag="IU325_04800"
     CDS             14595..15488
                     /locus_tag="IU325_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family protein"
                     /protein_id="HAP5808359.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVMTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVTATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15536..16426)
                     /locus_tag="IU325_04805"
     CDS             complement(15536..16426)
                     /locus_tag="IU325_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAP5808360.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGAGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLERGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(16623..17396)
                     /gene="hisP"
                     /locus_tag="IU325_04810"
     CDS             complement(16623..17396)
                     /gene="hisP"
                     /locus_tag="IU325_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HAP5808361.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17404..18120)
                     /locus_tag="IU325_04815"
     CDS             complement(17404..18120)
                     /locus_tag="IU325_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP5808362.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVMALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18117..18803)
                     /locus_tag="IU325_04820"
     CDS             complement(18117..18803)
                     /locus_tag="IU325_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311219.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP5808363.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18893..19675)
                     /gene="hisJ"
                     /locus_tag="IU325_04825"
     CDS             complement(18893..19675)
                     /gene="hisJ"
                     /locus_tag="IU325_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HAP5808364.1"
                     /translation="MKKLVLSLSLVLAFSSATVAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19896..20678)
                     /gene="argT"
                     /locus_tag="IU325_04830"
     CDS             complement(19896..20678)
                     /gene="argT"
                     /locus_tag="IU325_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HAP5808365.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20944..21513)
                     /locus_tag="IU325_04835"
     CDS             complement(20944..21513)
                     /locus_tag="IU325_04835"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP5808366.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVADVETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21608..23125)
                     /gene="purF"
                     /locus_tag="IU325_04840"
     CDS             complement(21608..23125)
                     /gene="purF"
                     /locus_tag="IU325_04840"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP5808367.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23162..23650)
                     /gene="cvpA"
                     /locus_tag="IU325_04845"
     CDS             complement(23162..23650)
                     /gene="cvpA"
                     /locus_tag="IU325_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HAP5808368.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23818..24480)
                     /gene="dedD"
                     /locus_tag="IU325_04850"
     CDS             complement(23818..24480)
                     /gene="dedD"
                     /locus_tag="IU325_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HAP5808369.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVESPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKEKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24470..25738)
                     /gene="folC"
                     /locus_tag="IU325_04855"
     CDS             complement(24470..25738)
                     /gene="folC"
                     /locus_tag="IU325_04855"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP5808370.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKSA
                     LLQRRGVEWNYSVTDHDWTFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25808..26722)
                     /gene="accD"
                     /locus_tag="IU325_04860"
     CDS             complement(25808..26722)
                     /gene="accD"
                     /locus_tag="IU325_04860"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HAP5808371.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26878..27537)
                     /locus_tag="IU325_04865"
     CDS             complement(26878..27537)
                     /locus_tag="IU325_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAP5808372.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAFN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27620..28432)
                     /gene="truA"
                     /locus_tag="IU325_04870"
     CDS             complement(27620..28432)
                     /gene="truA"
                     /locus_tag="IU325_04870"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP5808373.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28432..29445)
                     /locus_tag="IU325_04875"
     CDS             complement(28432..29445)
                     /locus_tag="IU325_04875"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP5808374.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29511..30647)
                     /gene="pdxB"
                     /locus_tag="IU325_04880"
     CDS             complement(29511..30647)
                     /gene="pdxB"
                     /locus_tag="IU325_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HAP5808375.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAIPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGITTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30746..31741
                     /locus_tag="IU325_04885"
     CDS             30746..31741
                     /locus_tag="IU325_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HAP5808376.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDIQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVC"
     gene            complement(31738..32916)
                     /locus_tag="IU325_04890"
     CDS             complement(31738..32916)
                     /locus_tag="IU325_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5808377.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFVLLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33181..34401)
                     /gene="fabB"
                     /locus_tag="IU325_04895"
     CDS             complement(33181..34401)
                     /gene="fabB"
                     /locus_tag="IU325_04895"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HAP5808378.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34560..36566
                     /gene="mnmC"
                     /locus_tag="IU325_04900"
     CDS             34560..36566
                     /gene="mnmC"
                     /locus_tag="IU325_04900"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HAP5808379.1"
                     /translation="MKHYAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAQWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQALPLPCATPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPEELAVAVEANAVEQITGVT
                     KNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIHYQYQLQDLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDKAVSAPVYDDLFMLAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAIKAG"
     gene            complement(36622..36900)
                     /locus_tag="IU325_04905"
     CDS             complement(36622..36900)
                     /locus_tag="IU325_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HAP5808380.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(36934..37482)
                     /locus_tag="IU325_04910"
     CDS             complement(36934..37482)
                     /locus_tag="IU325_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HAP5808381.1"
                     /translation="MNSTHHYEQLIEIFNSCFADDFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLENGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37482..38291)
                     /locus_tag="IU325_04915"
     CDS             complement(37482..38291)
                     /locus_tag="IU325_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HAP5808382.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38291..39115)
                     /gene="mepA"
                     /locus_tag="IU325_04920"
     CDS             complement(38291..39115)
                     /gene="mepA"
                     /locus_tag="IU325_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HAP5808383.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGLGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39119..40204)
                     /gene="aroC"
                     /locus_tag="IU325_04925"
     CDS             complement(39119..40204)
                     /gene="aroC"
                     /locus_tag="IU325_04925"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP5808384.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFEVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40239..41171)
                     /gene="prmB"
                     /locus_tag="IU325_04930"
     CDS             complement(40239..41171)
                     /gene="prmB"
                     /locus_tag="IU325_04930"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP5808385.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            41337..41888
                     /gene="smrB"
                     /locus_tag="IU325_04935"
     CDS             41337..41888
                     /gene="smrB"
                     /locus_tag="IU325_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HAP5808386.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVDEWLPPELP"
     gene            complement(42012..42881)
                     /locus_tag="IU325_04940"
     CDS             complement(42012..42881)
                     /locus_tag="IU325_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HAP5808387.1"
                     /translation="MSIYNMKKWTLFFASLMLLVLNVVGTPRSYAGDKLMSSSFDSVR
                     VYYAMDTVTGVAASIVFNVTVLTPGEVAYGKYNSIGRKGDTLRLISWTGSGPAPTLVI
                     TDYDTINNSHCPGIDTDLFRCAYMTFKVTVASDNYGCPWIASFYSYTNLPGYFDGSYT
                     SPTVHNSICPTIPVASYDISWSENYVSHNKALQLQSTGSTITTTLSTYLMENGKLCDG
                     SVFDSRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKELHDIVVKVNTSSRQPI
                     DSTCRFQYVLNEL"
     gene            complement(42868..43392)
                     /locus_tag="IU325_04945"
     CDS             complement(42868..43392)
                     /locus_tag="IU325_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP5808388.1"
                     /translation="MKKKCALIFINSLLLLNSGTTTAVDNLHFTGNLISKSCTPVING
                     SQLAEVHFTAIAASDLMNIGQSERVPLVIQLKDCQSSTSLSVRVTLTGTEDSELPGFL
                     AFDASSSASGAGIGIETAAGIPVPVNNTTGVTLPLTQGNNSLNFNAWLQAKSGRNVTS
                     GDFNATVTATFEYL"
     gene            complement(43389..43859)
                     /locus_tag="IU325_04950"
     CDS             complement(43389..43859)
                     /locus_tag="IU325_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP5808389.1"
                     /translation="MKRISLLVLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVFVDDINGSNYEQTVPYSITCDTAVRDPSMEMTLSWSGTQSDFDDAAVSSNITGLG
                     IQLKQAGQRFRINTPLVVNETDLPVLTAVPVKKSGVDLPEADFEAWATLQVDYQ"
     gene            complement(43856..44404)
                     /locus_tag="IU325_04955"
     CDS             complement(43856..44404)
                     /locus_tag="IU325_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP5808390.1"
                     /translation="MKSRAAKANDGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKIDGVSYNRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHSILDLTSGSWLPFNFSSGIPALEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(44379..45128)
                     /locus_tag="IU325_04960"
     CDS             complement(44379..45128)
                     /locus_tag="IU325_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAP5808391.1"
                     /translation="MTKYLSAKTLTTVLSLLAATVSFSAQASVTPDRTRLIFNESDKS
                     ISVTLRNNDPKLPYLAQSWIEDEKGVKISSPLAVLPPVQRIEAMMNGQVKIQGLPDIH
                     KLPADRESMFYFNVREIPPKSNKSNTLQIALQTRIKLFWRPKALEKNTTQNPWQHKVT
                     LTRNGQEFTVNNPTPYYVIFSNASAQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPVL
                     TYVNDYGARMPLFFTCNGNSCQVDEEQSRKG"
     gene            complement(45148..47787)
                     /locus_tag="IU325_04965"
     CDS             complement(45148..47787)
                     /locus_tag="IU325_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HAP5808392.1"
                     /translation="MPEHSFLRLRGLSWWIALAISGSPFNALADDTIQFDGRFLDLKG
                     NTKIDLGRFSQKGYVEPGKYNLRVHVNNQPLPDDYDIYWYATENDPNKSYACLSPELV
                     AQFGLKEDIAKNLQWIRDGQCLNTALLAGTEISGDLGQSALLVSVPQAYLEYTDSEWD
                     PPSRWDDGIPGLIADYSINAQTRHENGGDDTNDISGNGTVGVNVGPWRLRADWQSDYQ
                     HTRSNDDGDTDDSGTQKNWEWSRYYAWRALPSLKAKLSLGEDYLNSDIFDGFSYIGGS
                     ISTDDQMLPPNLRGYAPDISGVAHTTAKVTVTQMGRVIYETQVPAGPFRIQDIGDSVS
                     GTLHVRIEEQNGQVQEYDVSTASMPFLTRPGQVRYKVTMGRPQNWDHQVAGSFFSGGE
                     ASWGIANGWSLYGGALADENYQSAALGLGRDLALLGALAFDVTHSRVQLDDNSVYGNK
                     TLDGNSYRVSYAKDFDELNSRVTFAGYRFSEKNYMTMSEYLDANDDDRARTGNDKEMY
                     TVTYNQNFTDARVSVYLNYSHHTYWDRQDQTNYNMMLSHYFNLGSLRNLSVSLTGYRY
                     EYDKSADKGVYLSLSLPWGDNSTISYNGNYGSGADSNQVSLYHRIDDASHYTLSAGTS
                     ENHSSVDGYYSHDGTLAKVDLSANYHEGQYTSAGISLQGGATLTAHGGALHRTQNMGG
                     TRLLIDADGVANVPVEGNGSAVYTNMFGKAVVADVNDYYRNQAYIDLNKLPENAEATK
                     SVVQATLTEGAIGYRKFAVISGEKAMAVLRLQDGSHPPFGAEVKNDNQQQVGLVDDEG
                     NVYLAGVKPGEHMTVFWEGESHCDISLPDPLPNDLFNGLLLPCQQKGGSSPVIPHDIQ
                     PVIQEQTQQVTPMEPPMSVSSNQ"
     gene            complement(47871..48437)
                     /locus_tag="IU325_04970"
     CDS             complement(47871..48437)
                     /locus_tag="IU325_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP5808393.1"
                     /translation="MSKFVKAAVATAMLMGALTSASVVAAGNNGTVRFYGTIEDSPCS
                     IVPDDHKLEVDMGSIGTGSLTGGKTTTPKDFQIRLQDCNFNTETTMATTFTGNPYSTN
                     ADNYSLSNMDNGTEIPNVSLVIGDQHGTGYALGAEIKQPIVKDSSTGKGKPKQTLNFK
                     AWLVGETDAVTPTPAPFETLTTFQITYL"
BASE COUNT        12022 a        13020 c        11997 g        11549 t
ORIGIN      
        1 aagcacaaca aaaccttttt gaatattaaa taataattaa ttagcatcat cctcatacat
       61 taatcccgct taacaatagt ttcacacctt gcacccatta cgacacctcg gaaatcatca
      121 cgctgttaaa ctctccgtaa tgcggaaata tcatcatcac gattcattag tgaattctta
      181 aacgaggcat tttcaaacaa ttatcttaca gataaaaaaa ccacactcac aattaagaat
      241 cagaacaatt cactacataa cattaattat taagcatata cacctcatta ttttgccatt
      301 attaagttat taacagcaca atcaagcctt cccctctggc aaaatcttat tctgcagacc
      361 ttcaaaacgc cgtcctgggg gagtacattg ttctaagctg acttccacgg cagggagtgg
      421 cgataacagc aaaaaaggtc aagattcatg tcccccattg aaaaatccag caaattagag
      481 aatgtctgtt acgacatccg tggtccggtg ctgaaagaag caaaacgcct ggaagaagaa
      541 ggtaacaagg tactgaaact gaacatcggc aatcctgcac cattcggttt tgacgcgcca
      601 gatgaaatcc tcgttgacgt gattcgcaac ctgcctaccg ctcaagggta ttgcgattcc
      661 aaagggcttt actccgcgcg taaagccatc atgcagcact accaggctcg tggcatgcgt
      721 gatgttaccg tggaagatat ttacatcggc aatggtgtat cggagcttat cgttcaggca
      781 atgcaggcat tgctgaacag cggggacgaa atgttggttc ctgcacctga ttacccacta
      841 tggaccgcgg cggtttcgct ctccagcggt aaagcggtgc attatctttg cgatgaatcc
      901 tctgactggt tcccggacct tgatgatatt cgcgctaaaa ttacgcctcg tacgcgtggg
      961 atcgttatta tcaacccaaa taacccaacc ggcgcggtat attccaaaga gcttttaatg
     1021 gagatagtgg agattgcacg tcagcataat ctcattatct tcgccgatga aatttatgac
     1081 aaaattctct acgacgacgc tgagcatcac tcaattgcgc cgctggcacc tgatctgctg
     1141 accattacct ttaacggcct gtcgaaaaca taccgcgttg caggcttccg tcaggggtgg
     1201 atggtgttga acgggccgaa aaaacacgcc aaaggctaca tcgaaggtct ggaaatgctg
     1261 gcttcaatgc gcctgtgtgc taacgttcct gcgcaacacg ccattcagac tgcactaggc
     1321 ggttatcaga gcatcagtga atttattact cccggcgggc gtctttatga gcagcgtaac
     1381 cgcgcgtggg aattgatcaa cgatattccg ggcgtttcct gcgtgaaacc gcgcggtgcg
     1441 ctgtacatgt tcccgaaaat cgacgccaaa cgctttaata ttcacgacga tcagaaaatg
     1501 gtgctggatt tcctgttgca ggaaaaagtt ctgttggtgc aagggacggc attcaactgg
     1561 ccgtggccgg atcacttccg cattgtcacg ctaccgcgtg tcgatgatat cgagctgtct
     1621 ttgagtaagt tcgcgcgttt cctttctggt tatcatcagc tgtaatctta atttcactgc
     1681 cggagattgc atccggcagt gttatcccgc cacaatgacc tgatgatgtc atcatacata
     1741 aggtcactat gaaacagagc catttttttg cccatctctc ccgcctgaaa ctcattaacc
     1801 gctggccgct gatgcgcaac gtgcggactg aaaatgtgtc cgaacacagt ttgcaggtag
     1861 cgatggtcgc ccatgcgctg gcagctatca aaaatcggaa atttggcggt aatgtcaacg
     1921 ccgaacgtat cgctttactg gcgatgtacc acgatgcctc agaagtgctc accggcgatc
     1981 tccctactcc ggtgaaatac ttcaattcgc aaatcgctca ggagtacaag gcgattgaaa
     2041 aaatcgctca gcaaaaactg gtcgatatgg ttccggaaga gctgcgggat atctttgcgc
     2101 cgttaattga cgagcatgca tatagcgatg aagaaaaatc gctggtgaaa caggcagatg
     2161 cactgtgtgc atatctgaaa tgtctggaag aactcgcggc gggaaataat gaattcttgc
     2221 tggcaaaaac gcgactggaa gcgacgcttg aagcgcgccg cagccaggag atggactact
     2281 tcatggaagt ctttgttccc agcttccatc tttcgctcga tgagattagc caggattcac
     2341 cgctgtaagc agccggagtc tgcgtcgcat caggcaataa gcgccggatg cgacatcagg
     2401 ctcttgtcaa aacggaaaca gcatcgggat catcaccaca caaaccgcca tcacgataat
     2461 ggtgaacggt acccccaact tcacaaagtc actgaagctg taatttcccg gacctaaaac
     2521 cagtgtgtta accggtgaag aaaccggcgt cataaaggcg gcggatgctg ccatcgccac
     2581 gaccatcgca aatggataag gcgacacacc catcgttttg gcagcagcca gcgcaatcgg
     2641 agccatcaac accgccgttg cggtattaga gataaatagc ccaataaccg ccgacaagac
     2701 aaacaaacaa cccagcatca tatgtggccc gtaaccgccg ccaatgtcca tcagcccttt
     2761 caccgccagc gcgacacctc ccgttttctg taatgccaca gcaaacggca tcatcccaac
     2821 gatcaaaata atgctcggcc agtgaatgga tttataggcg ctttcagcat cgatacagcg
     2881 gaatttcccc atcagcaggc aggcgatgat agcggcaaca ggattaggaa tttcatcggt
     2941 cagcattaac gccaccatca acaccaggca aaaaatggca tgaggtgcct gactgtgtgc
     3001 tggcgatgct tcactcactt cttctggtaa gttcagcgcc acaaagtcac gcccttgttt
     3061 tgccagcata ccgatcagtt tccagttacc cacaaccagg atgatatcgc ccagcagcag
     3121 aggctcatcc gccagcgaac cttccatcgc ctcgccattg cgctttagcc ccaccacatt
     3181 cagtccgtag cgggtacgaa aaccaatttc gcgcaccgat ttaccaataa gttctgactc
     3241 aggaattaat gaaatctctg ccatgcccac atcaagtgcc tggtcagaaa aatactcgcc
     3301 gcgcagtacc atcggctcca gcaattgctc actacaaaat tgccggagat cgacatcagc
     3361 cgcagacata tcaataagca aaacgtcacg cgcgcgaaat tcagaaaccc cattaacgtt
     3421 cacgataacg cgacgaaaac gccgccagcg ttcaacaccg atgacgttag cgccataacg
     3481 ctcacgtaat ttgagatcgt ccagccgttg accaatcatt ggcgatccgg ggcgaatagc
     3541 cagacgtcgc gcacgcccgg tcagtcgata ttcacggata agatcgcgaa aggttcgacg
     3601 cgtccagcct tcgcgctgcg gggtctgggt atcccctttc agcatgaaac gcatcactaa
     3661 catatacaaa atacccagca ccagcacgac caggccaata ggtgttacgc taaagaaact
     3721 aaaaccatga tagccttcac gcagcaattc actgttgact accaggttcg gcggcgtcgc
     3781 caccagcgtc atcatgccgc ttatcagccc ggcaaaactt aacggcatca tcaggcgcga
     3841 cggcgacgtt tgcatacgca tggcaacgct taacaccacg ggaataaaga tagcgacaac
     3901 gccggttgaa ctcataaacg cgccaagccc cgcgacggtc agcatcaaca aaaccaacat
     3961 tttgatttca ctgttgcccg ccactttgac cagccatgtc cccattacgg tggcaacacc
     4021 ggtacggacc aaaccatcgc caataatgaa caaggcggca atcaggacaa cgttaggatc
     4081 ggaaaagccg gaaaatactt cggggaccgt cagcgttccg cttaatgcaa acgcgacaat
     4141 aacaaacaaa gcgaccgcat ccatacgcac tctgcccgtc gcaaacaaga cgatggcaac
     4201 ggccagtaat gaaagaaccc aaatcaattc accgttcaca acttatcctt gttaattgag
     4261 ggggatgact tgattctgcc ataaaaaagc cccgacgaga cggggctaaa tcatgatcag
     4321 gtgtttcact gaataataac atcgccattt ggctgtttgg tcacagtaat ttgctccaga
     4381 ctgtggagga ccaaatcgac ctcattcagg cgcggggtat ctgccggagc gttaaccgca
     4441 atgacatgac aacccgccgc caggccagaa agcacgccag cgggagcatc ttccaccacc
     4501 acacactcct gcggcgcaag ccccagcagc tgcgcgccta acagatacgc atcaggttct
     4561 ggttttccgc gcttcactcg ctcagcggtt acaaacacct ctggtgcggg aagcccagct
     4621 actttatggc gcgctcgcgc taccggcatg gagccagaag tcacaatggc ccacggaata
     4681 cctgctttat tcaaatgatt gagtaaggcg attgcccccg gaagcgcggt aataccctcg
     4741 gtttccgtgg cctcgatttg ctccagacac gtaaactcgg cggcaatatc agcctcggat
     4801 ttgcccgcca taaaatggcg cagagaggtg atcgcctgtt taccgtgaat gaaagccagc
     4861 acctcttccg gcgctaaccc atgacgtctg gcccagttgc tccacgcccg ctctaccgca
     4921 ggcagggaat ccaccagcgt tccatcaaga tcaaacagaa aacctttgca ccgcacgcgg
     4981 acctcctcag gcgttgataa tttgattaat ttcgttggcg ctcaaatggt actgacgcgg
     5041 gcaggcatgc cacacattaa gcatgcgctg atatttttcc cacattggcg tctgggcgtt
     5101 aaaaccgtga gttccagcgt caaaatgggt atagcgccct tccacattaa ccataaagcg
     5161 gacataaccg aggtaacgtg cttcagtggc ggcgtcaaag ccgaggaagg tgacacgacg
     5221 ttcatcgatg gattgctggt cctgcaaatt agaccaggaa acatgcaacg catgatacat
     5281 ctccataatg tcgatgatag tgcggcaggt ttcttctttc agctcgccaa actcgcgatc
     5341 cagttcacgc atctgtaatc cataaccacg ctcaatgatt gtttgcaggc gacggtaacg
     5401 ttcagcattt gccggatcga gcatagtcat catcttgtac tggttagaca aaataagacg
     5461 ttgcgcgttg gtcatttcca ttgttgactc ctgtatcact ctactacggt gaaaaaaaag
     5521 aaggctgagt atgccttctt ttatatgcgt aatcaggggt caattacaaa tcatcaagga
     5581 aagttttatc cagttgtttg aaggcgcgct taagcgtgtc agctaatgcc tggtaatcag
     5641 gcttgccttc aacgggtgcc aacacctgtc cagactcctg caatttaccg cgaacttcat
     5701 aaaaccagtt gaggatagca gggggtaatg gcgttacaga acgcttgccc agccaccaca
     5761 atccctgcat gggtaaactt aaggcgaaca gcgcagtggc aactgccggc ccaagctgac
     5821 cgcccagggc aatctgccag cagagagtaa atacggcgat cggcggcata aaacggatcg
     5881 cataacgcgt catcttgata acgcgatttt cgacaaagac cggggcaagg cgtttttcca
     5941 gcggccacgt ctttgagtaa tgctgtcccc ggcgaaacaa gctaaaaaaa ttaacagaac
     6001 gattatccgg cgttgacatg cttcacctca acttcacata taaagattca aaaatttgtg
     6061 caaattcaca actcagcggg acaacgttca aaacattttg tcttccatac ccactatcag
     6121 gtatccttta gcagcctgaa ggcctaagta gtacatattc attgagtcgt caaattcata
     6181 tacattatgc cattggctga aaattacgca aaatggcata gactcaagat atttcttcca
     6241 tcatgcaaaa aaaatttgca gtgcatgatg ttaatcataa atgtcggtgt catcatgcgc
     6301 tacgctctat ggctccctga cgttttttta gccacgtatc aattataggt acttccatgt
     6361 cgagtaagtt agtactggtt ctgaactgcg gtagttcttc actgaaattt gcaatcatcg
     6421 atgcagtaaa tggtgaagag tacctttctg gtttagccga atgtttccac ctgcccgaag
     6481 cacgtatcaa atggaaaatg gacggcaata aacaggaagc ggctttaggt gcaggcgccg
     6541 ctcacagcga agcgctcaac tttatcgtta atactattct ggcacaaaaa ccagaactgt
     6601 ctgcgcagct gactgctatc ggtcaccgta tcgtacacgg cggtgaaaag tataccagct
     6661 ccgtagtgat cgatgagtct gttattcagg gtatcaaaga tgcagcttct tttgcaccgc
     6721 tgcacaaccc ggctcacctg atcggtatcg aagaagctct gaaatctttc ccacagctga
     6781 aagacaaaaa cgttgctgta tttgacaccg cgttccacca gactatgccg gaagagtctt
     6841 acctctacgc cctgccgtac aacctgtaca aagagcacgg catccgtcgt tacggcgcgc
     6901 acggcaccag ccacttctat gtaacccagg aagcggcaaa aatgctgaac aaaccggtag
     6961 aagaactgaa catcatcacc tgccacctgg gcaacggtgg ttccgtttct gctatccgca
     7021 acggtaaatg cgttgacacc tctatgggcc tgaccccgct ggaaggtctg gtcatgggta
     7081 cccgttctgg tgatatcgat ccggcgatca tcttccacct gcacgacacc ctgggcatga
     7141 gcgttgacgc aatcaacaaa ctgctgacca aagagtctgg cctgctgggt ctgaccgaag
     7201 tgaccagcga ctgccgctat gttgaagaca actacgcgac gaaagaagac gcgaagcgcg
     7261 caatggacgt ttactgccac cgcctggcga aatacatcgg tgcctacact gcgctgatgg
     7321 atggtcgtct ggacgctgtt gtattcaccg gtggtatcgg tgaaaatgcc gcgatggttc
     7381 gtgagctgtc tctgggcaaa ctgggcgtgc tgggctttga agttgatcat gaacgcaacc
     7441 tggctgcacg tttcggcaaa tctggtttca tcaacaaaga aggtacccgt cctgcggtgg
     7501 ttatcccaac caacgaagaa ctggttatcg cgcaagacgc gagccgcctg actgcctgat
     7561 ttcacaccgc cagctcagct ggcggtgctg ttttgtaacc cgccaaatcg gcggtaacga
     7621 aagaggataa accgtgtccc gtattattat gctgatccct accggaacca gcgtcggtct
     7681 gaccagcgtc agccttggcg tgatccgtgc aatggaacgc aaaggcgttc gtctgagcgt
     7741 tttcaaacct atcgctcagc cgcgtaccgg tggcgatgcg cccgatcaga ctacgactat
     7801 cgtgcgtgct aactcttcca ccacgacggc cgctgaaccg ctgaaaatga gctacgttga
     7861 aggtctgctt tccagcaatc agaaagatgt gctgatggaa gagatcgtcg caaactacca
     7921 cgctaacacc aaagacgctg aagtcgttct ggttgaaggt ctggtcccga cacgtaagca
     7981 ccagtttgcc cagtctctga actacgaaat cgctaaaacg ctgaatgcgg aaatcgtctt
     8041 cgttatgtct cagggcactg acaccccgga acagctgaaa gagcgtatcg aactgacccg
     8101 caacagcttc ggcggtgcca aaaacaccaa catcaccggc gttatcgtta acaaactgaa
     8161 cgcaccggtt gatgaacagg gtcgtactcg cccggatctg tccgagattt tcgacgactc
     8221 ttccaaagct aaagtaaaca atgttgatcc ggcgaagctg caagaatcca gcccgctgcc
     8281 ggttctcggc gctgtgccgt ggagctttga cctgatcgcg actcgtgcga tcgatatggc
     8341 tcgccacctg aatgcgacca tcatcaacga aggcgacatc aatactcgcc gcgttaaatc
     8401 cgtcactttc tgcgcacgca gcattccaca catgctggag cacttccgtg ccggttctct
     8461 gctggtgact tccgcagacc gtcctgacgt gctggttgcc gcgtgcctgg ctgccatgaa
     8521 cggcgtagaa atcggtgccc tgctgctgac tggcggctac gaaatggacg cgcgcatttc
     8581 taaactgtgc gaacgtgctt tcgctaccgg cctgccggta tttatggtga acaccaacac
     8641 ctggcagact tctctgagcc tgcagagctt caacctggaa gttccggttg acgatcacga
     8701 gcgtatcgag aaagttcagg aatacgttgc taactacatc aacgctgact ggatcgattc
     8761 tctgactgcc acttctgagc gcagccgtcg tctgtctccg ccagcgttcc gttatcagct
     8821 gactgaactt gcgcgcaaag cgggcaaacg tatcgttctg ccggaaggtg acgaaccgcg
     8881 taccgttaag gcagccgcta tctgtgctga acgtggtatc gcaacttgcg tactgctggg
     8941 taatccggca gagatcaacc gtgttgcagc gtctcagggt gtagaactgg gtgcaggcat
     9001 tgaaatcgtt gatccagaag tggttcgcga aagctatgtt ggtcgtctgg tcgaactgcg
     9061 taagaacaaa ggcatgaccg aaaccgttgc ccgcgaacag ctggaagata acgtagttct
     9121 cggtacgctg atgctggaac aggatgaagt tgatggtctg gtttccggtg ctgttcacac
     9181 taccgcaaac accatccgtc cgccgctgca gctgatcaaa actgcaccgg gcagctccct
     9241 ggtatcttcc gtgttcttca tgctgctgcc ggaacaggtt tacgtttacg gtgactgtgc
     9301 gatcaacccg gatccgactg cagaacagct ggcagaaatc gcgattcagt ccgctgattc
     9361 cgctgcggcc ttcggtatcg aaccgcgcgt tgctatgctc tcctactcca ccgggacttc
     9421 tggtgctggt agcgacgtag aaaaagttcg cgaagcaact cgtctggcgc aggaaaaacg
     9481 tcctgacctg atgatcgacg gtccgctgca gtacgacgct gcggtaatgg ctgacgttgc
     9541 gaaatccaaa gcgccgaact ctccggttgc aggtcgcgct accgtgttca tcttcccgga
     9601 tctgaacacc ggtaacacca cctacaaagc agtacagcgt tctgccgatc tgatctccat
     9661 cgggccgatg ctgcagggta tgcgcaagcc ggttaacgac ctgtcccgtg gcgcactggt
     9721 tgacgatatc gtctacacca tcgcgctgac tgcgattcag tctgcacagc agcagtaatc
     9781 tcgtcatcat ccgcagcttt gcgctgcgga tatctgaacc ggaaatagtc actatttccg
     9841 gttttttatt ctcttaattt gcattaatcc tttctgatta tcttgcttag ctgcgctgca
     9901 tcaatgaatt gcgccatctc actttgcata cttaccactt tgttttgtgc aagggaatat
     9961 ttgcgctatg tccgcaatca ctgaatctaa accaaccaga agatgggcaa tgcccgatac
    10021 gttggtgatt atcttttttg ttgccatttt aaccagtctt gccacctggg tagttccggt
    10081 gggcatgttt gacagtcagg aagtgcagta tcaggttgat ggtcaaacaa aaacacgaaa
    10141 agtcgtagat ccacactcat ttcgcattct gactaacgaa gcaggcgaac ctgagtatca
    10201 ccgcgtacag ctgtttacga cgggcgatga acgcccgggc ctgatgaact tcccgtttga
    10261 agggttaacc tcaggatcga aatacgggac agccgttggc atcatcatgt ttatgctggt
    10321 gattggcggc gcgtttggca ttgtgatgcg tacaggaacc attgataacg gtatcctggc
    10381 gcttattcgc catacccgcg gaaatgaaat tctctttatt cctgcgctct ttattctgtt
    10441 ttcacttggc ggcgcggtat ttggtatggg agaagaggcc gtcgcctttg ccattatcat
    10501 cgcaccgcta atggtccggc taggctatga cagtattacc accgtcctgg tgacctatat
    10561 cgccacgcaa atcggttttg ccagttcgtg gatgaacccg ttttgtgtgg ttgttgctca
    10621 ggggattgcc ggcgttccgg tgctttctgg ctccgggttg cgcatcgtgg tgtgggttat
    10681 cgccactctg attggcctga tctttaccat ggtgtacgcc tcacgagtga aaaagaatcc
    10741 tcttctgtca cgcgtgcatg agtccgaccg cttctttcgt gaaaagcagg cggatgttga
    10801 acaacgtccg tttacctttg gtgactggct ggtattgatt gtcctgaccg ccgtaatggt
    10861 ctgggtgatt tggggcgtga tcgttaatgc ctggtttatt ccagaaattg ccagccagtt
    10921 cttcaccatg ggtctggtga ttggcatcat cggcgttgtt ttccgcctta acggcatgac
    10981 ggttaatacc atggcttcat ccttcaccga aggggcgcga atgatgatcg cccctgccct
    11041 gctggtaggt ttcgccaaag ggattttgct gctggtcggt aatggtgaag cgggtgatgc
    11101 cagcgtgtta aataccatcc tcaacagcat tgccaatgcc attagcggtc tggataacgc
    11161 ggtcgcggcc tggtttatgt tgctcttcca ggcggtattt aatttcttcg tgacgtccgg
    11221 ttctggtcag gcggcgttaa ccatgccgtt actggcaccg cttggcgatc tggtcggtgt
    11281 taaccgtcag gttaccgtgc tggcgttcca gtttggtgat ggcttcagcc acatcattta
    11341 cccaacctca gcttcgttaa tggcgacgct cggtgtgtgc cgggtggact tccgtaactg
    11401 gctgaaggtg ggcgcgaccc tgcttggact gctgtttatt atgtccagcg tcgtagtgat
    11461 cggcgctcag ttgatgggct accactaaaa atgttaagag ccgcaattgc ggctcttttt
    11521 tcattctgcc gtttcagtct ctacggcttc atttttggca ttgcgcttca tccacaacgc
    11581 cagcgctttc agcgagtctg gagtgaactc atcgcagcgt gcggtgattt cttccggtgt
    11641 cagccagcaa acttcactga cttcatcttc ctgtagggcg aagggaccgt gagagacgca
    11701 actgaacaac gcgccccaga cacggcaatt tttatcttcg aaatagaact gcccgtgctc
    11761 ggcaaagggg acaccggcaa tgcccaactc ttcttccgct tcgcgacgcg cggattccag
    11821 cagttgctca tcggcctgga ctacaccgcc tgcggtcgca tctaacatgc cgggtaaaaa
    11881 gtcttttgtc tcggtacgac gctggaccag aattttgccc atgccatcat gcacgacgat
    11941 gtaagttgca cgatgacgca gacactgtgc ccgcatttgt tcccggctgg cttgtgcaat
    12001 gacttcgttc tcttcattga caatatccac ccattcagta cttgccaaac gacgctgttc
    12061 cattatcagg aaaccttctt tttctggcgc tcttacggcg cattttgagt tgtgggtaaa
    12121 ttacggatta atcgcgacct gcgcaatgat actttgatca ttgagtgcga ttacactaag
    12181 tacgtcatta tccagcatgc cataactcgc cggattaccg cctttcggaa tactcaccga
    12241 gccggggttg aagtggaaaa tctccccccg ctgttctgcc actggcagat gggtatgacc
    12301 gtacaccagc acatcgttct ggtttaaagc aggcagattt tccggcccaa acagatgacc
    12361 atgcgtcaaa aacagacgtt gtttttccag taatacctgt tgccacggcg cggttatcgg
    12421 gaaatgcaaa agcatttgat ccacttcgct gtcgcagttg ccgcgcacag cgataacctt
    12481 atgtgccact tcattaagcc gttcagcgac tttggctggc gcgtaaccct ccggtaaagc
    12541 attacgcggg ccgtgattca acacgtcgcc aaggatcacc agccactggg caccgctttg
    12601 ggcaaacaac tccagaacac gttccgtcgc cggtaacgac ccatgaatgt ccgatgcaaa
    12661 catcagtttc atcactcact cctcgtcgaa aaaacgtaag ctatgatact ggattctgtc
    12721 gttgctatca gccagattgc ttcgccgaaa gtgcaataaa acgctggaca gacgctcgct
    12781 gccactggaa tgtcgcataa tccaccaggc gttccggcac ctcatcgcca tggagtacca
    12841 ggcggttaat cattagcgcc agatcagtat cagcaatgca ccattcacca aataaattcg
    12901 gctgacccag tgctaacaaa tgttctgcca tcgcgaacag tttctctgca ctggcttttc
    12961 cctccgccgt taatggcgct tttttcgcac ccgcaaagac aacatccgtt ggacgttcct
    13021 cgcggatggg catcagatcg ctacgcagcc aggcctgaat ctgtcgtgca cgcgcacgat
    13081 tttctaaatc aagcggataa atgcgttccc aggtcggtgg cgcaaatcga tcttccagat
    13141 actccgcaat ggcagaagat tcactcaact caaaatcatc aatttgtaat aacggcacac
    13201 ggcgtgtctg accgtaacct tgccacgtcg gctgcaaatg ttcaccgctg tcgaggtcga
    13261 tggtcttgat atgaaacgac aggccttttt cctgcaacgc cacccaggcg gataacacat
    13321 aaggggagaa aaaatgggca tctgaccaaa gcgtgatagc gggtttactc attatgtcct
    13381 cggcaataaa gggggctggc tatcaaacta tagagccttt ttgtcactgt cacgtgacga
    13441 atactcacgc aagtcgccgc gctttctata cttgtggtgt tcagatgatt agacaaccgg
    13501 agctgcaatg atcgatctct atttcgcccc gacaccaaat ggtcacaaaa ttacgctgtt
    13561 tctcgaagaa gcagggctgg attatcgctt gattaaggta gacctgggga aaggcggtca
    13621 gtttcgcccg gaatttttgc acatttcgcc taacaacaaa attccggcaa ttgttgatca
    13681 ttctccagcc gatggcggcg aaccgctaag cctctttgag tctggtgcca ttttgttgta
    13741 tctggctgag aaaacaggac tctttttgag tcatgaaacg cgtgagcgcg ccgccacatt
    13801 acagtggtta ttctggcagg taggcggact ggggccgatg cttgggcaga atcatcattt
    13861 taatcacgct gccccccaaa ccattcctta cgctattgaa cgttatcagg ttgaaactca
    13921 gcgtctgtac catgtactga acaagcggct ggaaaactcg ccctggctgg gaggtgagaa
    13981 ctacagcatt gcggatattg cctgctggcc gtgggttaat gcctggactc gccagcgaat
    14041 tgaccttgca atgtatccgg cagtcaagaa ctggcatgag cggatccgtt cgcgccctgc
    14101 caccggtcag gcactgctaa aagcacaact cggtgatgag cgttcggata gttaacagaa
    14161 acaggttctc gtgtattatt tcattctaag taaaacaacg gagaacctgc aatggcacaa
    14221 cctgccgcta ttattcgtat aaagaacctt cgtttacgta cgtttatcgg aattaaggaa
    14281 gaagaaatta acaaccgtca ggatattgtt atcaatgtga cgatccacta ccccgccgat
    14341 aaagcgcgca ctagcgaaga tatcaacgat gcgctgaatt atcgcaccgt aacgaaaaac
    14401 attattcagc atgtagagaa taaccgtttc tctttgctgg aaaaattaac tcaggatgtg
    14461 ctcgatatcg cacgtgaaca tcactgggtg acgtatgctg aagtggagat cgataaactg
    14521 cacgcgctgc gctacgctga ttcggtatcc atgaccttaa gctggcagcg ttaaccgcca
    14581 tatcgggagg ctgcatgaac atagtgatca ccggagggac gggattaatt ggtcgccatt
    14641 tgattccacg tttgctggag ctgggccatc aaatcacggt aatgacgcgt aacccgcaga
    14701 aagccagttc cgttctcggc cctcgggtga cactatggca agggcttgcc gatcaaagca
    14761 acctcaacgg cgttgatgcg gtaatcaatc tggccggaga accgattgct gataaacgct
    14821 ggactcacga gcaaaaagag cgtctctgcc aaagccgctg gaatatcacg caaaaactgg
    14881 tcgatttgat taatgccagc gacacgccac cgtcggtact catttccggc tcggcaacgg
    14941 gctattatgg cgacttaggt gaagtggtgg ttaccgaaga ggaaccgccg cataacgaat
    15001 ttacccataa actctgcgcc cgctgggaag aaattgcctg ccgggcacaa agtgacaaaa
    15061 cgcgagtgtg cctgctgcgt accggcgtag tgttggcacc ggatggcggt attctcggta
    15121 aaatgctgcc gccgtttcgt cttggtctgg gcgggccgat tggctccggt cggcagtatc
    15181 tggcctggat tcatattgat gatatggtca acggcattct ctggctgctg gataacgagc
    15241 tgcgtggacc atttaatatg gtttcgccct acccggtacg caatgaacaa tttgcccatg
    15301 cgctcggtca tgcgctgcat cgcccggcca ttttgcgcgt caccgcgacc gccattcgac
    15361 tgttaatggg cgaatcttca gtactggtat taggtggaca acgcgcgctg cctaaacggc
    15421 tggaagaagc gggttttgcg tttcgctggt acgatttaga agaggcgctg gcggatgttg
    15481 ttcgctgatg tggtttactg caaacatccg ccagttaact cctggtgtta cagaattagt
    15541 ggctttgcgc gataagatcg tctggtgaaa gtcgggtcac catcataact aactctctgt
    15601 ctaaacctct ctccagcatc tcctgagcaa tacgcagggc ttcttcgtgt ttgccctgca
    15661 ttgcgccttc ttcacgtaat ctgtcagcaa tagtcatcag tttctccttt tcttgtggtg
    15721 cgcgttccgc tatctcacca ataaatgcac ggaaacgctg ggcatctccc gtttgtaata
    15781 cataattaaa cagggctttt agctgcctgt cattagtgtt tcctgtaact agcagcgaaa
    15841 caatttggtc gactaatccc aacagatcgc gctgacgaat atgtttctga attaactcca
    15901 acagcgccat tttgcgatgt tgcataatct cgtcatccgg caccacggta atatccacca
    15961 gcggaaaagc cgatgaatat attttgcggg ctatagcagg ctcggcaaat tcatcaagcc
    16021 agcagagtga ataaggataa ggacttctgc aaccatgata aaacagcatc gggatcacca
    16081 atggaagctc tttatagcct gcatcaagat ggttttgcat cgccgcaatg gaatagcgca
    16141 tcatgcgaaa agccattaat tcttccggct tactttggtg ctctatcact acataaatat
    16201 aacccgctcc ctcctgcgtt ttcacagacc acaagaggtc ggaataatat tgccgcaggt
    16261 cttcatcaat aaaactgttt ggttccagtt taagcgttgt taaatcacac agtttgcgca
    16321 gcggcgcggg aagatgaata tcaataaaat cccgcgcggt gtctggatgg cgtaaaaaag
    16381 atttaaatac cgcatcatgc ggcgtggaag ttgtcgatat tgtcatggcg ttccgtcacc
    16441 cctcaaattt aagataacgc gacaataacc acgattaaca cccaatgcag caggctgttt
    16501 ttatctttgg acagcgaatt cagaactatt tttagcggac tgcaactttt acatttcagc
    16561 ggaagctgac tcgcaaattc cccttgaaaa tactccggca gcgttagcca ccggaggggg
    16621 aattatttca gcgatccctt aaggaactgt tgcagacgag ggctttgcgg gttaccaaat
    16681 aactgttctg gcgcaccctc ttcttctatt ttcccctgat ggaggaaaat gacgtgagta
    16741 gaaacatggc gagcaaagcc catttcgtga gtcactacca ccatggtttt cccctcttct
    16801 gccagttgct gcataatacg taacacctcg cctaccagtt caggatcgag cgccgaggta
    16861 ggttcatcaa acagtaaaac ttccggttcc attgccagcg cccttgcgat ggaaacacgc
    16921 tgttgctgac caccggacag atgcaccggg tatttcccct gcgcacgttc gtctatccca
    16981 acttttgcca gatacttcac cgcccgctcg cgcgcttcct gcttgctcag gcccaacacc
    17041 tgaatcggcg cttccatgac gttttccagc accgtcatat ggctccagag attgaagtgc
    17101 tggaatacca tcgtcaggcg tgtgcgcagt aagcgcagct gatttttatc ggcgactttg
    17161 agttgaccgt ctttgtcgcg caccagattg atcgtctggc cattgaccac gatcgatcct
    17221 tcactcggtt tttcgaggaa gttaatgcag cgcagaaagg tacttttccc cgatcccgac
    17281 gatccgatga tgcttatgac atctccggca ttcgcttgca gtgagacccc tttcagcact
    17341 tcgtgttcgc cgtagcgttt gtgcaaatcg ataacgttta atttattctc ggacatcgta
    17401 ttctcagtgc gttgaagaag gtttcacatg ctgcaaccag cgtttttccg ctctgcgaaa
    17461 gaggctgatc aggacataag agatgattaa atagagcacc gcggcaatgc cgaaggcggt
    17521 aaaaggttga tacgtggcgg cgttaatatc gcgggctatt ttcagcagat ccggcacagt
    17581 agcggtgaaa gccagcgcag tagagtgcag catcaggatc acttcgttgc tgtaggccgg
    17641 taacgcaata cgcagcgccg atggcaaaat aatgcagcga tacattttaa aggtcgagaa
    17701 gccataggct ctggcggctt cgatttcccc atgcggcact gaacggatcg ctccggcaaa
    17761 aatctcggta gtgtaagcgc aggtgttgag cgtcagcgcc agcacggtac agttcaggcc
    17821 actgcgaaaa aaggcgttaa ggaactcggt tcccttaaca atttccagcg tgtacatgcc
    17881 ggaatagaac accagcaact gaacatacag cggcgtaccg cgaaaaatat acgtaaataa
    17941 ccagattgga aactggatgt atttattact tgagacgcga ccaatcgcca gaaacagcgc
    18001 catgactccg cctatcacta ccgacaaaat cagcagccac agggtgatcg ccacgccagt
    18061 aaagcgataa ccgtcagtcc acagcagcgg tttccagtat tcatgtaaga tttcgatcac
    18121 aggtcagccc tcttcacacc cacggagtag cggcgctcaa ggaacagcag cacaccattg
    18181 gaaacagtgg tgaaaaccag gtaaatcacg ccacagacga tggcgaaata gaacggttcc
    18241 caggtacttt tgcctgccag ctgcgtggct ttgaccacat cttccaggcc gagtaacgaa
    18301 acgagtgcgg tagatttgag gatcacctgc cagttgttgc caatgcctgg cagggcgtaa
    18361 cgcatcatcg ccggaaacat gatccgccga aacacttgcc cacgagtgaa accgaacgcc
    18421 gtcgccgcct ctatatgtcc tttcggcact gccataaaag cgccacggaa cgtttcggta
    18481 aaataagcac cgtagataaa accgagagta ataataccag cgaccatcgg atcgatatca
    18541 atctgcccga cgcccatcgc ctccgtcacc gtgtttagtg caatctgcaa accgtagaaa
    18601 attagcagca tcaacaccaa gtccggcacg cctcgaatca gcgtggtgta cccttcgaaa
    18661 atcagccccg aaagccgatt ttgcgagagc ttaccgccag cgccaattaa accgatgatt
    18721 acagcgagca ctacagagct gatagccagc tccagcgtga cgagcgcacc ctgtaaaata
    18781 acacctgaaa acccatacaa catgctgcct gtcctgtcgt gtgtggtgaa ttaccgcctc
    18841 ttgtctccct ccggttaccc gaagggagcg gactttcttt caccgatggc gattaaccac
    18901 cataaacatc aaaatcgaag tactttttcg ctaatttctc gtaagtaccg tcagcgcgca
    18961 tttcggcaaa ggctttgttc agtgcctcgc gcagttcgtt atcttcttta cgcaggccca
    19021 tgccggtccc tacgccaaac agtttttcat ctttaacaga cgggccaccg aatttgtaat
    19081 ctttaccgac gggttgtttg aggaaacctt cgctggcagc gacctcatcc tggaacgcgg
    19141 catcaatacg tccggcagtc aggtcagaat aaatattgtc ctgcccttga taagaaacga
    19201 tttcaatgcc ttttggtgcc cagtgttcgt taccgaacgt ctcctgggtg gtgccctgca
    19261 atacgcctac ccgtttgcct ttcagcgact cgactgtcgg ctgaatgtcg gaatttttcg
    19321 ccaccaccag acgagaatcg gcagcgtaca gtttgtcggt gaaggctatt tcttgctgac
    19381 gtttttccgt aatggaaagc gatgacatga tggcgtcaat cttcttcgct tttaaggacg
    19441 ggattaacgc atccagtggg ttttcgacaa acgtacattg cgtattgatg cgtttgcaca
    19501 actccttcgc cagatcgata tcgaagccaa ccagttcgcc ttgtgaattt tttgattcaa
    19561 atggcgcata ggtcgggtcg gtaccgatgc ggatgttttg cggaatcgca gcaaacgcca
    19621 cagttgcgct ggagaaggcc agaaccagag agagcgatag caccagtttt ttcataatgt
    19681 cctcaactga cagtcttttt atagggaata tttacaggtt tgttacactt atcgtgccat
    19741 aaaattgacc atcaaggcaa aatatcccgc cccgttagac gtattatttt tcattaaaaa
    19801 tgcttaagta tgcatttcaa tgcaccacga tagtgcaaca aacctggcgt gcaccatgat
    19861 gatcattcca tcaggtacag cttccctgcg acgtatcagt caccgtagac attaaagtca
    19921 aaatactttt tcgccatctt gtcgtaggtg ccgtcctgac gcagctcacc aagcgcctta
    19981 ttgaaggcag ccgtcagttc agcatcatct ttacgtagcc ctacaccggt gccgtcaccg
    20041 aagtattttt tgtctttcac tgatgagcca gcaaaggcga agtctttacc agcaggttgc
    20101 ttgaggaatc cttcgctggc agcaacttca tcttgtaacg cagcatccag acgtcctgca
    20161 gccagatcgg aatagaccaa atcctggttg gcataggcca ccacatccac acctttgcta
    20221 cgccaggtct cgttagcgta agcttcctgg gttgatccct gcagcacacc aacatgttta
    20281 cctttcagtg aatccagcgt tggctgaatc ggcgaacctt tggcggcaat caaacgagaa
    20341 tctgcggcgt acagcttgtc ggagaaagca atctcctgct gacgtttatc ggtaatggaa
    20401 agcgacgaaa taatggcgtc gatttttttc gctttcagtg aggggatcag cgcgtcaaag
    20461 tcactggcaa cccaggtaca tttcacctgc atccgtttgc acatctcgtt accgagatcg
    20521 atatcaaagc caacaaaatc acctttagca tctttcgatg agaacggcgc ataggtggta
    20581 tcagttccga tacgtaccgt ctccggtagc gccgcatagc tggaagccgc tgcggagaga
    20641 ccgactaaca aagacagagc gagaatcgac ttcttcatac ataaccctca agtgaaaatg
    20701 gcttttttat gttgtgtatt gtgttgtgtg tttgcaggct ctttcatgca ggtcttatgc
    20761 catcttgacg acaacagtaa cattcaacgt taaatatgtt aataagacgt tgcattattg
    20821 tcctgaagtt gaagatagca ggtatagcgg ttgaatcgca gcgtttcgat tgaatggcag
    20881 aaacaaaatg tcgaggattt gatcgcggtt gcaaaattgc cctgaaacag ggcaacagcg
    20941 gagttatgcg ccctgccagc gggcaaagag atcttcagga agcgttatgg cgaattgatc
    21001 gagtacacgg ttaaccgtct gatttatcac atcatcaagg gattgcgggc gatgataaaa
    21061 cgccggaacg ggaggcataa tcaccgcacc gatttctgct gcctgagtca ttaaacgcag
    21121 atggcctaag tgcaatggtg tttcacgcac gcagagcacc agcgggcgac gctctttcag
    21181 cacaacatct gccgcacggg tcagtaagcc atcagtatag ctatggacaa tgccggacag
    21241 ggttttgatc gaacagggta aaatcaccat ccccagcgtc tggaaagaac cagaagagat
    21301 gctggcagca atatcgcgcg catcgtgcgt cacatcagct aacgcctgca cttcgcgcag
    21361 ggaaaaatcc gtttcaagag ataaggtctg gcgcgcggcc tggctcatta ccagatgcgt
    21421 ttcaacatct gcgacatcgc gcagaacctg taataagcgc acgccataaa tcgcgccgct
    21481 ggcaccgctg atgcccacaa taagtcgttt cataaatagt tgccctgtca gacttgcagg
    21541 cagactttgc aggatttcgg cgggagttgc aagtcagggt gccagaccgg caccctgagc
    21601 gaaggcatca tccttcgtta tgcatttcga gattttccac ttcgttctga cgttgcacgg
    21661 ctttggcgtc atcattacgt aaagtatcaa ggaaatcgag gtagccctga tcaacatctt
    21721 tggtgacgta gacgccgttg aacaccgagc attcaaactg ctggatatcc gggttttcag
    21781 cgcgaacggc ttcgatcaga tcgttcagat cctggaaaat caacccgtca gcaccgatga
    21841 tctggcgaat ttcatctact tcgcgaccgt gagcgatcag ttccgtggcg ctcggcatat
    21901 caataccata aacgttcggg aagcgaattt ccggtgccgc agaagcgaga tacactttct
    21961 tcgctccggc ttcgcgtgcc atctcgataa tctgctcaga agtggtgcca cggacgatgg
    22021 agtcgtcgac cagcaggacg tttttatcgc ggaactcggc gcggttggcg ttcagtttac
    22081 ggcgcacgga cttacgacgc agctgctgac ccggcatgat gaaggtacgg cctacatagc
    22141 ggtttttaac gaagccctga cggtacggtt tgcccagaat acgcgcgatt tccagcgcga
    22201 tatcacaaga ggtttccggg atcgggatca ccacgtcgat atccagatct tcccattcgc
    22261 gggcaatttt ctcgcccagt ttcgtgccca tattcacgcg ggcgctgtaa acggaaattt
    22321 tgtcgatgaa cgagtccggg cgagcaaagt atacatactc aaacaggcac ggattgctga
    22381 ccggattgtc agcacattga cgggtaaaca actgcccttc ttcagtgatg taaatcgctt
    22441 cgccaggcgc gacgtcacgc aggaaatcaa agcctagtgt atcgagcgca acgctttcgg
    22501 aagcgaccat atattctgtg cggttctcgt caatatcacg ttttcccagt accagcggac
    22561 gaatcccgtt tggatcgcgg aaagcaacca taccatggcc gataatcatc gccacgcagg
    22621 catacgcgcc gcggattaag cggtttgtgg cagcaatggc agcgaaaata ttgtcggctt
    22681 ccagcgggta gtggcggaag ttgtccagct cgctggcgaa gatattaagc agaatttccg
    22741 agtcggaagt ggtgttgatg tggcggcgtt tttcttcaaa cagtttttta cgtaactcgt
    22801 gagcattggt cagattgccg ttgtgggcaa gcgtaatgcc atacggagag ttaacgtaaa
    22861 acggctgcgc ttcagaggcg ctggagctgc cagccgtagg gtaacgcaca tgaccaatgc
    22921 ccatattgcc ctgcaaacgc tgcatatggc gagcttcaaa tacatcgctc accagcccgt
    22981 tcgctttacg caaacggaag cagttattgg catctatggt gatgatgccg gcggcatcct
    23041 gaccgcgatg ctgaagcacc gttaaggcat cataaatcga ctggttaacc ggcataacac
    23101 cggcgatacc gacaataccg cacatacgtc tttttcctcg ttaagccaca tctcagagca
    23161 cttacgctct gggcaagaaa cttgacgagc tttgcagata atcaaaaaac catctgatga
    23221 taaaactgaa ctgcgggatc agctgtgatt tgctccagtc ttcgcttttc gacaccccgg
    23281 taaaggagtc gagaaagaag agaatggcgg caacaatcaa cacaccgcgc aacgcaccga
    23341 aacagacgcc cagcacccga tcggtgcctg acaaccccgt tttctccacc aactggccta
    23401 tcacgaagtt cacgatagca ccaacaatca gggtagcgat aaacagtacc gcgatggcaa
    23461 tcccatttcg aaccagttcg tcttcaaagc ccgtaaacca gactgacagg taagtgtagt
    23521 aatgactggc aacaaagaaa gcacaacccc atgtcaccag cgataacgct tcacgaacaa
    23581 agccgcggat caggctaacc agagaggaaa aagcaatcac cgcgattatg gcgtaatcaa
    23641 tccagaccat atgtgtccca cgattttacg ccctgtcatc ctgttcgggg cgcattctaa
    23701 cagaaaaaga aaacgtttgc gtagggattt ccttcccacg catcaataaa aatggcgctg
    23761 aaaaaatatt caacgccatc gactttttat gcctttgcgg catcgggcaa gaccgtatta
    23821 attcggcgta tagcccatta ccacgccact taagccagaa agctgcttga gctcacccag
    23881 cgatcctttc agcttctctt tggaagcatc aggcccaacc agaatacggg taattttacc
    23941 ctgcactggc gtggatggcg acgtataaac ccgataaccg gcaccgcgca gcttaccgac
    24001 aatctcattc actttatcgg catttttcag cgcgcccagt tgcacaacat aagctttacc
    24061 cgttggcgct gctttttctt ccacgaccgg cttcggttct ggcttcggcg caggtggtgc
    24121 ttcaaccttc ggtttaggcg actccaccgg tttcggcttc ggtggggcga ccggtgcagg
    24181 ttccggttca aactcggtgt tattggctgc aatagtggcc ggatcgagcg acggtgctgc
    24241 ggcatcacct gcccgcacct cttccgctgc gccttccggc ggctgcgtcg gcagcgcctg
    24301 ggtggcggct ggcatcatat caggctcatc gcgatcgccc gcttttggca ccagcgggat
    24361 agccgcgaac tcatcctgat aatgtttttt ctgcccgtcc agcagccctg gaagtacaat
    24421 cacccccagc gccaccagca cgatcgtgcc cactaaccga ttctgaaact tacttgccac
    24481 cgcttctcct cgcgtcaatc acttccatga catgtgcgac cgtgtggaac gaaccacaca
    24541 ccagcacggt atcttccgct ttagcgtccg ccattgcggc atcccatgcc tgcgccacgc
    24601 tatcaaatga tttgccgtta cccaaatgct caagcagttg ttctgccgtg gcaccgcgcg
    24661 gcccttccag tggcgcacaa taccagtcat caaccacgct tttcaaccag gccagagttc
    24721 cggcaatatc tttatcatgt agcataccga taaccgccag cacgcgcccg tttttcggta
    24781 gcgctttcat acgcccggtg agatattccg ccgcatgtgg attatgcgcg acatcaaaaa
    24841 taacacgtgg cgactcgctc acaatctgga aacgtcccgg caaaattgcg ctggcaatcc
    24901 cgtcgcgaat ggcattttca ctgacctcca gcccgctggc acgcagtgcc gccagcgccg
    24961 ttgcggcatt cggttgcggg acaagcggca acggcagatt ttccagcgtg ccgtgagcat
    25021 cgctaaacgt ccagtcatga tcggtgacgg aatagttcca ctcaacgccc cgacgttgta
    25081 acagtgcact tttttcctgc gccacatcag caatggtaga aggcatttcc ggctcaccga
    25141 caattgccgg tttttcgctg cggaagatgc ctgctttctc gcgaccaatg ctttcgcgat
    25201 ctggccccag ccagtcggta tgatccagcg caatgctggt tacaaccgcg acatcggcat
    25261 cgacaatatt ggttgcgtcc agacgaccac ccagccctac ttccagaatc accacgtcaa
    25321 gttgtgcctg cttgaacagc cacaacgccg acagcgtacc gtactcgaaa taggtcaggg
    25381 aaatatcacc gcgtgccgat tcaatctccg caaaagaggc ggtgtgggcc gattccggca
    25441 attcctgacc ctgcacacgt acgcgctcgg tataacgcac cagatgcggt gaactgtaga
    25501 cgcccacttt gtaccctgcc gccatcagaa tcgactccag cgtacggcag gtggtgcctt
    25561 tgccattcgt acccgcaacg gtaaacacaa atggcgctgg tttcagaacg ccaagacgcg
    25621 ccgcgacctg gctcacgcgc tcaaggccga gatcgatagt tttactgtgc aggttttcca
    25681 gataagaaag ccacgaagcc agaggcgacg cggcttgagg agtgcgtttg ataatcatgg
    25741 tatccgctga ttcgttacgg tgagaatagc aaaagggcag agccagtggc cctgccctta
    25801 tcagttatca ggcctcaggt tcctgatctg gtaccggtgg caccactacg ccttcacgcg
    25861 gcgcttcagg attcggcgct ggcagattca tcaacttcgc cagaatactc gccagtttca
    25921 ggcgcatttc cggacgacgg acgatcatgt cgatcgcgcc cttctcgatc aggaattcac
    25981 tgcgctggaa tcctggcggc agtttttcgc gaacggtctg ttcgataaca cgcggaccgg
    26041 caaagccgat taacgctttc ggttcagcga tgttgagatc gcccagcatg gcgaaacttg
    26101 cagaaacacc acccatggtc gggtcggtca gcacggagat gtacggcaag ccgcgctcct
    26161 gcatttttgc cagcgccgca gaggttttcg ccatctgcat cagcgacatc agtgcttcct
    26221 gcatacgtgc gccaccagag gcggagaagc agatcagtgg gcagttatct tccagcgcct
    26281 gctcaacggc acgcacgaaa cgtgcaccta ccacagaccc catggaaccg cccataaagg
    26341 cgaactcgaa tgccgcagcg acaactggca ttccatacag ggtgcctttc atcaccacca
    26401 gcgcatcttt ttcgccggtt tctttctgcg cagatgccag acggtcttta tacttcttgg
    26461 agtcacgaaa cttcagcaca tctttcggct caagctcgct acccagctcc acaaggcttc
    26521 cttcatctaa caggctatgc aggcgattac gcgctgtcat acgcatgtga tggtcacact
    26581 tcggacagac ctcaagatta cgttccagct cagcgcggta taaaacctga ccgcagctat
    26641 cacacttagt ccacacccct tcaggaatgc tcgccttgcg ggtgggagta atgttgcttt
    26701 taattcgttc aatccagctc attagggacc tttctgtctg aacctggttc gatgccagtt
    26761 ttatctttgg ggacgcataa tgccattttt gcccccaaca gaccatgaat gttgcacatt
    26821 aaaacataac agcccgaaac tttggataaa aaagtggtcg aaccgcggag ttacttttta
    26881 ttttgcggcg cgtgccgcag cgcgtttgtg gcgaataaat tcgaaaacac ctggcaatat
    26941 tgaaacaaaa ataatgccaa caatcatcag tttaaggttg ctctgaatga aggggagcgt
    27001 tccgaagaaa taacccgcgt aggtaaaaat cagtacccat agcaacgcgc cgatgacgtt
    27061 aaaagcggcg aaatgacgat acgacatgtg gcccattccc gcaacgaacg gcgcgaacgt
    27121 tctgacgatc ggcacaaaac gggcgagaat aatcgttttg ccgccatgtt tctcgtaaaa
    27181 ctgatgagtc ttatcaagat aactgcgacg aaagattttc gaatccggat tactgaacag
    27241 tttgtcacca aataaccgtc caatggtgta gttcaccgcg tcacccacaa tcgcggcgat
    27301 cagcatcagc accaccatca tatggacgtt gagatcgttg gtttccagtg atgccaacgc
    27361 accggcaaca aacagcaatg aatcacccgg taaaaacggc gttaccacca gaccggtttc
    27421 acagaacaaa attaagaaca aaatggcata aacccagacg ccatactccg cgaccagttc
    27481 tgccagatgc acgtcaatgt gcaggataaa atcgatgagg aaataaatca ggtccatatt
    27541 agcctatgcc ttgtactcgt catcttacag gctgtaacta cgttggctac agcctgaaat
    27601 gttccgagca ttattcttgt tagtccgcca gaaatagcgg gcccattggc ggttttggaa
    27661 gatcataccg gtcagggtaa tccaccgcga ccagatacag cccttccgct tttgccgttg
    27721 ctgccgccag cgttctgtcc tttgccgcca gcaactctgc tatccagctc tccggctggt
    27781 tgtgggcacc gacttccatc aggctgccga caatattcct gaccatatga tgtacaaagg
    27841 cattcgcttt gatatctacc accacataag gaccgtgacg cgtgacatta atatgcataa
    27901 cgttgcgcca cggggttcgg gactggcact gcaccgcacg gaacgaggtg aaatcattct
    27961 cgcccagcaa gcattgcgcg gcccgatgca tccgttcagc gtccagcggt tcgtaaaaat
    28021 gggttacccc tttactcagt accgccgggc gcagccgatg attgtagatg atgtagcgat
    28081 aacggcgagc cgtggcgcta aatcgggcat gaaaatcatc aggtacagct ttaacccaac
    28141 gcacggcgat gtcaccaggt aaattcgcat ttacgcccaa cgtccacgca gcgtctttgc
    28201 gctgcgcggt cgtttcgaaa tgcacaacct gcccggtgcc gtgtacacct gcgtcagtac
    28261 gcccggcgca gaagacggtg atgggttcgt tcgccacctg ggagagcgcc ttttccagct
    28321 tctcctgcac actgcggact tcgttctgcc gttgccagcc gtaatactta ctgccgtcgt
    28381 actcaatgcc cagcgcaatt ttataaactg gcggttgttg ctggtcggac attagtacag
    28441 atactcctgc accagtttct cggcgatttt tactgccatc agcgcgccgc caaagcgaac
    28501 gttatcggcc accgaccaga actgaacttg ctccggcata ccgtagtcat tacgtacgca
    28561 gccaacggaa agatgcggcg taccagaagc atcccccacc tgagtcggga attcgttctc
    28621 ttcagagagc acaatatctt cgccctgagc aaacgcatca cgcgcttctt ctgctgccag
    28681 cggacgcagt gcttcaaagt tgaccatctg ggcatgaccg tagaataccg gtgcctggac
    28741 gacgcttgcc gaaatcatca gcccttcgtc ctgcaggatt tttcgtactt cgtcaacgat
    28801 acgacgttct tcacgcacgc taccttcgct atccggcagt aacggcagca tgttgaacgc
    28861 cagctgacgc ccgaagaaat cttcttcatc aatcggaatg ccgttgagca atttcgcact
    28921 ctgccccgct aacgcatcga ccgctttttt gccctgggcg gaggctgaaa tcaggctggt
    28981 aacgctgata cgcgacaaac cgccctgatc gattaacggt ttcagtgccg ccagcagttg
    29041 gctggtcaga ctgtccggta cggcgatgac attccggttg cggtaatcgg tcagtacaaa
    29101 cgggtttact tccggcacca ccagcggtac gtcaggttcg agagcaaaca atccgctgct
    29161 atcgatcacc aggcaacctg agttggtcgc ttcttcaacc caggcagcgg tagcttcttt
    29221 gcctgcgaca aaaaacgcca gctgcgcctg cgtccagtcg aattcagcgg catcctgcac
    29281 ggtgattgtc ttaccaccaa agcgcagttg ttcgcctgcg ctttcgttac gtgccagtgc
    29341 ataaatttcc ccaaccggga actgacgttc agccagcgtt tcaagcaggg cttcgcccac
    29401 agcgccagtt gcgcccagga cggcaatgtt ccagccttca gacatggtgg tttactccag
    29461 aaatagcaaa gctccctgcc aatattacag cagagagcat gaagaagaga ttaacgtgcc
    29521 ggatgatgaa cggcgttaaa acccagttta cacagcaatg atgccgcgct ggcgtcatca
    29581 caaatcacat acagagatga ccattcacgg cgctcaagat agtttttgcg cagcttatcg
    29641 aactcacccg gtattccggc gactttacgc agcggtgcgt catcgcggcg cacatcatac
    29701 accaaatgca ccagcctttt cagcgtcggt tgatcgagcg gaccatgcag cgtaatgcgg
    29761 ccaaactctg gcgcgggcag caacgtatcc agcgcaacat gctgttcatg cccaataaac
    29821 ttgctatagg cttcaaacac ttgcgtagta ccgcgtgctt taccttccag ggtatagcct
    29881 gcgatatgcg gcgtgccgat atccactttt ttcagcaatt ccacgttgag ttccggttcg
    29941 ccttcccaga catccagcac tacgcttaac ttctggcctt cgttcagaca ggtcaatagg
    30001 gcagtattat cgacgactgc gccacggcag gcgttaatca gaatcgctcc tggtttcagg
    30061 ctacggatca gtttttcatc cgccagatgc agcgttttgt acggaccatc tttaaagagt
    30121 ggcgtatgga aagtcagaat atccgcatgc tgaactaatt catccagcga gcggaaatcc
    30181 ccctcatccc cacggtcggc gcgaggcgga tcgcaaagca aggttgtaat ccctaacgct
    30241 tccagtcgcg cctgtaaacg gcgaccaacg ttaccaacgc ccacgatccc cactgtacgg
    30301 tcgtgcagtg aaaatccatc gcgttcggca agcatcagca gtgaggagaa aacatattcc
    30361 accaccgcaa tcgcattaca gcctggtgcc gcggaaaaac caattcccgc ctgctttaac
    30421 cacgcttcat cgacatggtc ggtccccgcg gtggcagtgc caacaaattt aatgggtttt
    30481 cctgccagca aagattcatt cactttcgtg accgaacgca ccatcagcgc atccgcgtct
    30541 gccagttgag ccacggggat tggacgcccg ggaatcgcgg tcacctcgcc caaacggcta
    30601 aataagtcgc gggcataagg catattttca tcaacaagga ttttcacgtt tgtgttacct
    30661 gtatgagacg agagttaacc ggacaagtgt gccataatct cgcggccagg catacttgcg
    30721 aagatttcag gtataaggat acgtaatgat acaacctatt tccggccctc ctcctgggca
    30781 accaccaggt cagggagata acctgccgtc tggcgcgggc aatcagcctt tatccagtca
    30841 gcaacgtact tcgctggaaa gcttaatgac gaaagtgacc tcactgacgc aacagcaaag
    30901 agcagaactg tgggcgggta tcaggcacga tattggtctg tcgggagatt caccgctgct
    30961 ttcgcgtcac tttcctgccg ctgagcataa tctggcgcaa cgtctgctgg ccgcgcaaaa
    31021 aagccattct gcccgccagc ttttagcgca attaggggag tatctacgtc tggggaataa
    31081 tcgtcaggcg gtcacggatt atatccgtca taactttggt cagacgccgc tgaatcagct
    31141 ctcaccggag caattaaaaa ccattctaac cctgttgcag gaagggaaga tggttattcc
    31201 gcaaccgcag cagcgcgagg cgacagatcg tccgttatta ccggcggagc acaatgcgct
    31261 caaacagctg gtgaccaaac ttgcggcggc aacgggagaa cccagcaagc agatctggca
    31321 atcgatgctg gaactttccg gggtgaaaga tggcgagtta attccagcga aactgtttaa
    31381 ccatctggtg acctggctgc aggcgcgtca gacgctaagc cagcaaaata cgccgacgct
    31441 tgaatcactc cagatggcgc taaaacaacc tttagatgcc agtgaactgg cggcgttatc
    31501 ggcatatatc cagcaaaaat atggcctttc tgcgcaatca tcgctttctt ctgcccaggc
    31561 cgaggatatt cttaatcagc tttatcaacg gcgggttaaa gggattgatc cgcgagatat
    31621 acaaccgctg cttaatcctt ttccaccgat gatggacacg ttgcaaaata tggcaacgcg
    31681 tcccgcgctg tggatactgt tagtcgcgat tatcctgatg ctggtctggc tggtttgtta
    31741 accccgacga aacgacagta tcgtgacaat aattcccaga accgcagaga tcgccccggc
    31801 aagaaatacc gaagagtaac caaacgtggt cgccagcatt cccgcaagcg gcccggagac
    31861 gccgagggcg atatcctgaa acgcggcgta accgcccagt gcggtgccgc gaacttgtga
    31921 ggggacgcgt ttaaccacct ccacgcccag cgcaggaaag ataagcgaac atccggctcc
    31981 ggttaacgcc gcgcccgcta atgcgaccca cgcacctggg gcttgccaga gcagcaacaa
    32041 gcccaccgtt tctacaagca gagagacaat cgccactttc acgccgccaa aacggtccgg
    32101 catccagcca aacatgacgc gcatcacgac aaatgcaccg ccaaacgcgg taagagtaaa
    32161 gcccgccatt gcccatcctt tgctggcaaa gtagagcgaa acgaaagtcc cgataaccgc
    32221 aaaaccaacg ccttgtagtg ccagacctaa ccctggtttc cagataagcc cgacaacgct
    32281 ccacagcgat ggacgttctc ccgccagggc cggtactttg cgcactgtgc cgttacaggc
    32341 ccacgccagt aagggtaatg ccattgtggt gagcgccagt gcggcaaaac cgtaatggct
    32401 atgaatcaac aggccaagcg gcgcaccaac agcgagggca ccgtaaatcg ccattccgtt
    32461 ccatgacatc actttgccgg aatgttttgg cccaacgatg cctaatcccc aggtcagagc
    32521 gcccgtcagt aactggcttt caccaaagcc aagaatcaaa cgcccgacga ccaacaggac
    32581 aaatttgaac ggtgcggaga cgggcaaaat cgccgccaac agcagcgcgc cgccagccag
    32641 accacaagct aacatcccct gaagcgccga acgttttgcg ccatattgat cggccagtcg
    32701 cccggcgtaa ccacgcgtca gcaccgtagc cagaaactga atgccgacgg caataccgac
    32761 catggtgttg ccatagccca gttcatgatg aacaaacagc gggataaccg gcaacggcag
    32821 ccctacggtc atgtaggtga gaaaaaccgc aaaagcgatg cggaagagcg aaaaattggc
    32881 agaagatcgt gtttcggttt ggcttacagc agtcatgcat tactccagaa tgcagcgcaa
    32941 ggcgaggaat ttccccgtct catctctctg gtttcagggt tacggtgcgt tggcaggatt
    33001 taacgcgcac gtcttttcag aaggaaatcg acaaagcggg aagtttgcct ggaacaggcg
    33061 gcgattgtca atgatgtgaa aaagggaacc atcgggttcc ctttggcgtt agtgccggag
    33121 gccgcattgg cgcgtaatgt cggatgcgac gctgacgcgt cttatccgac ctactccaaa
    33181 ttaatctttc agcttgcgca ttaccagcgt ggcgttggtg ccgccgaagc cgaagctgtt
    33241 agacataacg gtggtcagtt cgcgatcggt cgtttcggtc acgatgttca gacccgcagc
    33301 ctgttcgtcc agctcttcaa tgttgatgct cggggcgata aagccgtgtt ccagcatcag
    33361 cagagagtag atagcttcct gtacgccagc agcgcccaga gagtgaccgg tcattgcttt
    33421 ggttgcagaa atcgccgggc tcttatcgcc gaacacttca cggatagccg ccagctcttt
    33481 cacgtcgcca accggagtcg aagtaccgtg ggagttcagg tagtcgattg gggtatcaac
    33541 accgtgcatc gccatcttca tgcaacgcac tgcgccttcg ccagacggag caaccatgtc
    33601 tgcgccatca gacgttgcgc cgtagccaac gatttcagca tagatgtgag caccacgcgc
    33661 cagcgcgtgt tccagctctt caaccactac cataccgccg ccgcctgcga taacgaaacc
    33721 gtcacggtga gcgtcgtaag tacgggaggc tttttccggg gtgtcgttgt atttggtaga
    33781 cagcgcaccc atcgcgtcga attcgcaagc catttcccag cacagctctt cgccgccacc
    33841 agcaaacacg atgtcctgtt tgcccagttg gatctgctct actgcgttac cgatacagtg
    33901 tgcggaagtc gcacacgcgg agctgatgga gtagttaacg ccatgaattt taaacggcgt
    33961 ggcgagacag gcagaaacgc cagatgccat cgctttagtg accacatacg ggccaaccgc
    34021 tttcaggccg cgcgggccgc gcattgcgtc agcgccgaac acctggaaac gcggggaacc
    34081 accaccggaa cctgcaatca ggcccacacg tgggttgttc tgataagctt ccggggagag
    34141 gccagcatct gcgattgcct gctccatgga aaggaatgca tagatggatg cgtcgctcat
    34201 aaagcgcaca actttgcggt caatgaggcc agtggtatcc agttttacgt tgccccatac
    34261 gtggctacgc atgccggagt ccttcagctc ttgagagaaa gtgatccctg agcgtccttc
    34321 acgcagagat gccaggactt cctgctggtt attaccgatg ctggaaacga tgcccaggcc
    34381 agtaatcact gcacgtttca ttcaatacct ctgtaagtcg cactatagta agtttcgaat
    34441 ggcacaatag cgtacacttg tacgccgaac aagtccgatc agacgattaa tagagaaatt
    34501 tgcgcagcct gacacacatc gttaagatcg tcagactgcc cgtcagacga gtaacttacg
    34561 tgaaacacta cgccatacaa cctgccaacc tcgaatttaa tgctgagggt acacctgttt
    34621 cccgagattt tgacgacgtt tacttttcca atgacaacgg actggaagag acgcgttatg
    34681 tttttctggg aggtaaccaa ttagaggtac gctttcctga acatccacat cctctgtttg
    34741 tggtagcaga gagcggcttc ggcaccggat taaacttcct gacgctatgg caggcatttg
    34801 atcagtttcg cgaagcacat ccgcaagcgc aattacaacg tttacatttc attagttttg
    34861 agaaatttcc cctcacccgt gcggatttag ccttagccca tcaacactgg ccggaactgg
    34921 ctcagtgggc agaacaactt caggcgcagt ggccaatgcc cttgcccggt tgccatcgtt
    34981 tattactcga tgaaggccgc gtgacgctgg atttatggtt tggcgatatt aatgaactga
    35041 ccagccaact ggacgattcg ctaaatcaaa aagtagatgc ctggtttctg gacggctttg
    35101 cgccagcgaa aaacccggat atgtggacgc aaaatctgtt taacgccatg gcaagactgg
    35161 cgcgtccagg cggcacgctg gcgacattta cgtctgccgg ttttgtccgc cgcggtttgc
    35221 aggaagccgg attcacgatg caaaaacgta aaggctttgg gcgcaaacga gaaatgcttt
    35281 gtggagtgat ggagcaagca ttacccctcc cctgcgccac gccgtggttt aaccgtacgg
    35341 gcagtagcaa acgggaagcg gcgattatcg gcggcggtat tgccagcgcg ttattgtcgc
    35401 tggcgctatt acggcgcggc tggcaggtag cactttattg cgcggatgaa gcccccgcgc
    35461 tgggtgcttc cggcaatcgc cagggggcgt tgtatccgtt attaagcaaa cacgatgagg
    35521 cgctaaaccg ctttttctct aatgcgttta cttttgctcg tcggttttac gaccaattac
    35581 ccgttaaatt tgatcatgac tggtgcggcg tcacgcagtt aggctgggat gagaaaagcc
    35641 agcataaaat cgcacagatg ttgtcaatgg atttacccga agaactggct gtagccgttg
    35701 aggcaaatgc ggttgaacaa attacgggcg ttacgaaaaa ttgcagcggt attacttatc
    35761 cgcaaggtgg ctggctgtgt ccggcagaac tgacccgtaa tgtgctggaa ctggcgcaac
    35821 agcagggttt gcagattcat tatcaatatc agttacagga tttatcccgt aaggatgatt
    35881 gttggttgtt gaattttgca ggtgatcagc aagcgacgca tagtgtggtg gtactggcga
    35941 atgggcatca aatcagccgt ttcagccaaa cgtcgtctct gccagtgtat tcagttgctg
    36001 gtcaggtcag ccatattccg acaacgccgg aactggcaaa gctgaagcag gtgctgtgct
    36061 atgacggtta cctcacgccg caaaatccgg cgaatcaaca ccattgtatt ggtgccagtt
    36121 atcatcgcgg cagcgaagat acagcgtaca gtgaggacga tcagcagcag aatcgccagc
    36181 ggttgattga ttgtttcccg caggcgcagt gggcaaaaga ggttgatgtc agtgataaag
    36241 aggcgcgctg cggtgtgcgt tgtgccactc gcgatcattt gccaatggta ggcaatgttc
    36301 ccgattatga ggcaacactc gtggaatatg cgtcgctggc ggagcagaaa gataaggcgg
    36361 taagcgcgcc ggtttatgac gatctcttta tgcttgctgc tttgggttcg cgcggtttgt
    36421 gttctgcccc gctgtgtgct gagattctgg cggcgcagat gagcgacgaa ccgattccga
    36481 tggatgccag tacgctggcg gcgttaaatc cgaatcggtt atgggtgcgg aaattgttga
    36541 agggtaaagc gattaaggcg gggtaatcta ctttggcagt gtttgccgga tgcggcgtga
    36601 acgccttatc cggcacatta cttactgctg tgacgcctgc tgaaacaggt tttcccacat
    36661 atcggtcacc agtgcctggt cacgcggcga caattcaccg gcaccaatgg ctttttccag
    36721 actctggcta acggttgtat gtaccgcctg ggcggagtgg tcatcaccac tttccagttc
    36781 tgcgatggct aatgtcaggt ggccacgcaa atacccactg gcaaacaact catcatcact
    36841 ggcatggtca accataccgt cgattaatgc cagaatgcgt gattcaaact ccgcgatcat
    36901 cttctttcct cattgtaaaa cgtggcgcag gcttcagttg agcgcttccg gccacgggaa
    36961 ctgttccgcc gtaagttccg gcgtgtgata ataattttgt aatgctttaa tgaagcgtgc
    37021 cgggcgttca ggaatgccgt tttccagata atccatcacc tgtgcatgta cccgccgttg
    37081 aaataccacg cggtccggtt cgaaatcccc ttccagattg tcgcaactga cattaaacgg
    37141 atatcccgcc gccacacaga acaaccagtc cagcgcctgc ggcttcactt caacatcttc
    37201 aaactggctt tgcgtttggg catcgcgtcc gtcgggacaa taccagtagc cgaaatcaac
    37261 cagctcacgg cgcgctttcc cggcaataca ccagtgcgaa atctcatgaa ttgcgctggc
    37321 ataaaagcca tgagcaaaga cgattcggtt atacggtact tccgcatcag caggaagata
    37381 gatcggttcg tcgtcgcctt taatcagacg ggtattaaaa tcatcggcaa agcagctatt
    37441 aaaaatttca atcaactgct catagtggtg tgtactgttc attagttcat ccccaaccag
    37501 tggaggatct cctgtccgtg gctatcgtaa agtaatttgg cactcatcac cgccgagacg
    37561 ataacgatca tcgggcggat cagcttttgt cctttgctca acaccagtcg tgaacccatg
    37621 cgcgcgccga ggaactgccc caccagcatc acgaatcctg tcgcccaaat cactttgcca
    37681 ccgagaataa acagcagtaa accgccgata tttgacgttg cgttgagtaa tttggcgtga
    37741 gccgtggctt tggcgaggtt gaatccgcac agcgtaacaa aggccagcgc gtaaaacgat
    37801 cccgctgccg gaccaaagaa gccgtcataa aaaccgacgc agccaccggc aattaacgcg
    37861 aagggtaaac cgtacatccg gcgctggcga tcttcttcac caagctttgg catcagcaag
    37921 aaatagaggc cgatacaaat caccagaatg ggcaaaatct gccgtaagac atcagcctga
    37981 acatactgca ccagcaatgc gccgctcatt gagccgacaa aggtcatggc gatattgagt
    38041 ttctgatcgc ttaaactaac cactttgcgg cgaataaagt agatagtggc ggaaatagag
    38101 ccgccgcagg cttgcagttt attggttgcc agcgcgttag cgggagacat ccccgccgcc
    38161 atcaatgccg gaatggtgag taacccaccg ccaccggcaa tcgagtcgat aaatcccgcc
    38221 agcatggcga caaaaaagag aactcccagc aacagcgggg aaaccataaa caggctatta
    38281 aacgtttcca ttagatcacg tgctcatcca gtagcgcctg gcaggaaggc ggcaacggag
    38341 gcggtgtctt cttctcaggc tttgttgttc ccggttttgg aggttcaaac cagctttgca
    38401 gttctgcccc gcaaccatcg cctggtggcg gtaaaggttg atcttcacac tccagactat
    38461 cggcaggaca acgtaatcgt acatgcatat gcgcgcgatg ctggaaccag ggtcgcactt
    38521 tgcgcaacca gtcgcgatcg gtgcccgcat caaggcaaag ttgttgttta atcgccggat
    38581 taacaaaaat gcgcgtgacg tctttgtcct gggcggcgag tttgatcaag ctgaaaattt
    38641 ctggcttcca cagcgtcgga acaacgtgtt taccgtcgcg ggaaactaaa tctaacgctt
    38701 gcgggcgcaa gagctgcgcg gaggtccagc gtgtttttgg cagttgcagg aagatgtcca
    38761 catccagtcc ggtctggtgg ctggcgtgac cgccgttgaa acgaccacca gcaggcatcc
    38821 ccatatcgcc aatcagcacc gtgcccaagc ccagattgct cacctggcta ctcagacgct
    38881 ggataaacat caccagatcc ggatgtccga aatagcgacg ctgatcggta cgcatgacct
    38941 gataatgttc ggactgtatc ggcagcgtgt cagcgccgac gatacaacca ttagaaaaac
    39001 tgcctatcga ttgtgcgcta cccggcacag gttgggttat tttttgccac ggcgtcgctg
    39061 ccaggctggc gctactggca agcagggcca gcagcgcaat cgcggttttg ttcatttttt
    39121 accagcgtgg aatatcagtc ttcacatcgg cattttgcgc ccgctgccgt aacaggtgat
    39181 ccattaaaac gatcgccagc atcgcttctg cgatcggcac cgctcggatc ccgacacaag
    39241 gatcgtgacg gcctttggtg atcatctcaa cttcttcgcc aaagcggttg attgtgcgcc
    39301 ctggcacggt aatgctggag gttggcttca gcgccatatg ggcaatgatt tgctgcccgc
    39361 tgctgatacc gccaagaatg ccgcccgcat ggttgctctg gaaaccgtct ttggtgattt
    39421 cgtcgcggtt ctggctgcca cgcagcgcta ccacttcaaa accatcacca atttctacgc
    39481 ctttcaccgc gttgatgctc atcagcgcat gggcgatgtc ggcatccagg cggtcaaaga
    39541 ccggctcgcc aagtccggcg gggacgccac tggcaacaac ggtgactttc gcaccgatgg
    39601 agtcgccctc ttttttcagc gcgcgcatca gttcatctaa cgcgtcgatt ttgtccgggt
    39661 ccgggcagaa aaacggattt tgctcgacct gcgaccagtc tttgatttcc agcggaatgt
    39721 cacccatctg ggtcaggcag ccgcgaatct caatgccaaa tttctcggcg agatattttt
    39781 tggcaatcgc ccctgccgcc acgcgcatgg cggtttcgcg ggcggaagag cgcccgccgc
    39841 cgcgataatc gcgcagaccg tatttttgtt cgtaggtgta atcggcatgg cctggacgga
    39901 aaacgtcttt aatggcgctg taatcctgag aacgctggtc ggtattttcg atcaacaagc
    39961 caatgctggt gccagtagta acaccttcaa aaacaccgga gagtattttg acctgatccg
    40021 gctcgcggcg ctgggtggta tagcgcgatg tcccagggcg acgacgatca aggtcatgtt
    40081 gcaggtccgc ttccgtcagc ggaatgcctg gcggaacacc atcgacgatg cagccgagcg
    40141 ccagcccgtg cgattcgccg aaggtggtta cgcgaaagag ttgtccaatt gtgtttccag
    40201 ccatcacggc tccgttattg ttgtgtttgc gtgtttactt aatctttata aatcgcgaaa
    40261 tgttcacggg ccgccagcag ctgctctttg gtgagcataa acacaccatc gccgccgtta
    40321 tcaaactcca gccaggtgaa cggaacatcc ggatattgtt ccataagatg taccatgctg
    40381 ttgcccactt cacaaatcag cacgccatca tcagcgaggt aatctgccgc gtttccgaga
    40441 atgcgacgcg tcagtttcag gccgtcggtg ccagatgcca ggcccagttc cggctcgtga
    40501 cggtactcat ttggcaggtc ggacatatct tccgcatcga catacggcgg gttagtgaca
    40561 atcaggtcgt actgcacttt cggtaaatca cggaacagat cagagcgaat cggaatgacg
    40621 ttgtggatca gaccgtgttc ttcgatgttc tgttcagcaa ccgccagcgc gtcgggagag
    40681 atatccaccg cgtcaacttc cgcatccggg aaggcataag cacaagcaat ggcgatgcag
    40741 ccgctaccgg tacacatatc taaaatatgc tgcggttgct tgcttatgag tccggcaaat
    40801 ttattgttaa tcagttcacc aatcggcgag cgcggcacca gcacacgttc atcgacgtaa
    40861 aattcatgac cgcagaacca cgctttgttg gtcaggtaag ccaccggaat gcgttcattg
    40921 acgcggcgga tcacgcgttc aacaatacgg tgtttttcgc tggaggtcag acgcgcggtg
    40981 cgcatatctt ccgggatatc cagcggcagg tagagcgaag gcaacaccag ctgtacggct
    41041 tcatcccacg ggttgtcagt gccgtgaccg taccagatat tcgccgcgct gaagcggctc
    41101 accgaccagc gcaacatgtc ctgaatggtt tgcagctcat ttactgcttc atcaacgaaa
    41161 attttatcca cgtattcctc cagggcatga tcgcaataat ttcggcggct agtttgccat
    41221 gaagatgacg ataaatcagc attcacgcgc ggtgagtgag gaaaaatacg tttaaaacga
    41281 tcgattgcgc tacgagtcgg gtaaactgta ggaaaattag aaatagagac agataaatga
    41341 aaaagaaaac aacacttagc gaggaggacc aggctctgtt tcgccagttg atggcgggga
    41401 ctcgcaagat taagcaggac acgattgtcc accgaccgca gcgtaagaaa atcagcgaag
    41461 tgccggtgaa acggttgatt caggagcagg ccgatgccag ccattatttt tccgatgaat
    41521 ttcagccgtt attgaatacc gaaggtccgg tgaaatatgt tcgcccggat gtaagccatt
    41581 ttgaggcgaa gaagttacgc cgtggcgatt attcaccgga gctgtttttg gatttgcacg
    41641 gtttgacgca gctgcaagcc aagcaggagt taggcgcatt gattgccgcc tgtcgccgcg
    41701 aacatgtgtt ttgtgcctgc gtgatgcatg gtcatgggaa gcatattttg aagcagcaaa
    41761 cgccgctgtg gctggcgcaa catccgcatg tgatggcatt tcaccaggca ccaaaagagt
    41821 acggtggtga tgcggcattg ctggtgttga ttgaagtgga tgagtggttg ccgccggaat
    41881 taccgtgaat gctggcatcg aatgccggat gcggcgtgaa tgccttatcc ggccgataaa
    41941 aatgtgataa ttcaataaat tgtgattcat ttcagtcggc gtcacatcag gcatcagtgc
    42001 acaacgacga gctataactc attcaataca tactgaaaac gacacgtaga atcaatcggt
    42061 tgccgactgc tggtattcac cttcaccacg atatcatgta actctttatc ggttaccgga
    42121 tgacgtgtgg gggtcaccgt taccgttgat ttgtcgcagc cataagaggt aaaggttaac
    42181 agttctgata ccgcacggca gtatgcacct ctactatcaa aaacgctgcc gtcacagagt
    42241 ttgccgtttt ccatcaggta ggtcgaaagc gtggtggtaa ttgtcgaccc cgtggactgt
    42301 agctgtagcg ctttattatg actgacataa ttttcactcc aggagatatc ataggaggcg
    42361 accggtatcg ttgggcatat tgagttatgc acagtcgggc tcgtgtagct tccatcaaaa
    42421 tatccgggta agtttgtata tgaataaaag gacgcgatcc acgggcaacc ataattatca
    42481 gatgcaacgg tgactttaaa tgtcatatat gcacaacgga aaagatccgt atcaataccg
    42541 gggcaatgtg aattattaat cgtatcataa tccgttataa ccagagttgg tgccgggccc
    42601 gaacccgtcc aggaaatcag cctcagagta tctcctttac gcccaataga attatatttc
    42661 ccatacgcga cctcccctgg tgttaacacc gtaacattga aaacgatact cgcggccacc
    42721 cctgttaccg tgtccatcgc ataataaact ctcacactgt cgaaacttga actcataagt
    42781 ttatccccag catagctcct gggtgtgccg acaacattca ggacaagcag catcaggctc
    42841 gcgaaaaata gggtccattt tttcatgtta taaatactca aaggttgccg tcacagtggc
    42901 gttaaagtca ccggacgtta catttcgccc gctcttcgcc tgtaaccagg cattaaaatt
    42961 taggctgtta tttccctgag tcagcgggag cgtgacgcct gttgtattgt tgactggcac
    43021 tggtatacct gccgctgttt caattccaat cccagcgccg gacgcagaag acgaggcgtc
    43081 aaaagccaga aatccgggca gttcactatc ttctgttccc gttaacgtga ctctgacact
    43141 cagcgatgta gaactttgac aatccttcaa ttgaatgacc aacggcactc gctctgactg
    43201 cccaatgttc attaaatcgc tggcggcaat ggcagtaaaa tgtacctctg ccaactggct
    43261 accgttgatc actggtgtac acgatttact gattaaattg ccggtaaagt gcagattatc
    43321 taccgctgtt gtggtgccgc tgttcagcaa cagcaggcta ttgataaaaa tcaacgcaca
    43381 ctttttcttc attgataatc cacctgtaac gtggcccagg cttcaaagtc cgcctcaggc
    43441 agatcaacac cactcttttt tactggcaca gccgttaaca ccggtaaatc ggtttcattc
    43501 accacgagtg gcgtattgat cctaaaacgc tgcccagcct gtttaagctg aatacctaat
    43561 ccagtgatat tgctgcttac tgcggcatca tcaaaatcgc tttgtgtgcc actccagctc
    43621 aaggtcattt ccattgatgg gtcgcgaacc gccgtatcac aggtaattga atacggcacc
    43681 gtttgctcat aattactgcc attaatgtca tcaacaaaca catcctgaaa ggtgatttct
    43741 atggtttgag cgttgctaat agtgcagtta ggcggctgca ctaaatagcc gtgaaaagag
    43801 acatttgaga gtgccatcga gcaaaagccc cacagcacaa gaagtgagat ccgtttcatt
    43861 gataatccac cacaattgtg gcgctggcgt taaattccgc tgccgctaag gttgtaccac
    43921 tctgtttcac cggcacggct tcaagtgcag ggatgcccga ggaaaaatta aacggcaacc
    43981 atgaaccaga cgttaagtcg agtattgagt gatcactact tttttgaacc cgaataccaa
    44041 accctggaat acctgtgctg ataactgttt caccgttaat cacgaccgtt gtcgcctgca
    44101 tctgtaaacg caagtcatcc attgccaaat tgttgcaaac aagcgagtag tcaatgggac
    44161 ggttataact caccccatcg atcttattaa tgaatacatt accaaactca actgccgcac
    44221 catttaccgt acaaggagga ggggcatcaa caagtacccg taaattcata cttactgact
    44281 tactcgctgc aactgcaaag ccgctaccaa cacaacatat cagtaaccca gttaccgccc
    44341 agacagaaca agatcctttc atactggctc tcccgtcatt agcctttgcg gctctgctct
    44401 tcatcaacct ggcaactatt gccgttgcag gtgaaaaaga gcggcatccg agcgccatag
    44461 tcgttgacat aggtcagtac cggcacactc cccatcttca cattcagcgg tgcagaggtt
    44521 ttcggcgaca tcactatagg cgagaaaccg gctgccggat tgccattttt ctgtgcgctg
    44581 gcattactga aaatgacgta atacggcgtc gggttattga cagtaaattc ctgaccgttg
    44641 cgggttaagg tcactttatg ctgccacggg ttttgcgtgg tgtttttttc cagagctttc
    44701 ggtcgccaga aaagtttgat acgcgtctga agagcgattt gtagcgtgtt ggatttgttc
    44761 gatttcggcg gaatttcgcg cacgttgaaa tagaacatcg attcccgatc ggcaggtaat
    44821 ttatgaatat ccggcaggcc ctgaatttta acctggccat tcatcattgc ttcaatgcgc
    44881 tgtacaggcg gcaataccgc cagcggcgag ctgattttga cgcccttttc atcttcaatc
    44941 cagctttgcg ccagataagg cagtttgggg tcgttattac gcagcgtcac gctgatagat
    45001 ttatcgcttt cattaaaaat caggcgagta cgatcgggcg ttactgacgc ctgagcagaa
    45061 aaacttaccg ttgctgccag taacgacagt accgttgtca atgttttggc agagagatat
    45121 ttagtcatgg atcattcctt aatcacgtta ttggttcgat gaaacagaca tcggcggttc
    45181 cattggtgtc acctgttgtg tctgttcctg aatgacgggt tgaatatcat gcggtatgac
    45241 gggagaactt cccccctttt gctggcatgg caacaacaag ccattgaaca ggtcattcgg
    45301 taacgggtct ggcaggctga tatcgcagtg agattcaccc tcccagaaaa cggtcatatg
    45361 ttcacccggt ttaacgcctg ccagataaac attaccttcg tcatcaacca accctacttg
    45421 ttgttgatta tcgtttttta cttccgcccc aaatggcgga tggctgccat cctgcaagcg
    45481 cagcactgcc atcgccttct caccgctgat taccgcaaat ttacggtagc caatagcgcc
    45541 ttccgtcagc gttgcctgaa ccactgattt tgtagcttcg gcgttttccg gcagtttgtt
    45601 caaatcgata taagcctggt tgcggtagta atcattcaca tctgccacca ccgctttgcc
    45661 gaacatgttg gtataaacgg cggaaccatt accttcaacc ggtacattag caacaccatc
    45721 agcgtcgatt aacagacgtg tgccgcccat attttgtgta cgatgcagtg ccccgccatg
    45781 agcggtcaac gttgccccgc cctgtaaaga gatccctgct gaggtgtatt gcccttcatg
    45841 gtagttagca ctgagatcaa cttttgccag agtgccatcg tggctgtagt agccatccac
    45901 gctgctgtgg ttttcgctgg ttccggcgct taacgtgtag tggctggcgt catcaatgcg
    45961 gtggtacagg ctcacctgat tgctgtcggc accgctgcca tagttgccgt tgtaactgat
    46021 ggtgctgttg tcgccccacg gcaaacttaa ggacagataa acacccttgt cggcactttt
    46081 atcgtattcg tagcgataac cagtcagaga aacgctcaag ttacgaaggc ttcctaaatt
    46141 gaaataatga gaaagcatca tgttgtagtt ggtttgatcc tggcgatccc agtaggtatg
    46201 gtgggaataa ttgagataga ccgaaacgcg cgcatccgtg aagttctggt tataggtgac
    46261 cgtatacatc tctttgtcgt taccggtgcg cgcccggtcg tcgtcgttcg catccagata
    46321 ctcgctcatg gtcatgtagt ttttttcaga gaagcggtat ccagcaaacg tcacccgact
    46381 gttgagttca tcaaaatctt tggcatagct gacgcgataa gagttgccgt ccagcgtttt
    46441 gttgccatac acgctgttat cgtcgagttg aacacgagag tgagtgacat caaacgctaa
    46501 cgcgcctaac agcgccaggt cacgaccgag gcccagcgcc gccgactggt agttttcatc
    46561 ggctaacgca ccgccgtaga gcgaccagcc attggctatc ccccacgacg cctcgccgcc
    46621 cgagaagaag ctacctgcaa cctgatggtc ccagttttgt ggacgcccca ttgtcacttt
    46681 ataacgcacc tggccggggc gagtcaggaa cggcatggac gccgtgctga cgtcgtattc
    46741 ttgtacctgg ccgttttgtt cttcaatgcg gacatgcagc gtgccagaga cggagtcgcc
    46801 aatatcctga atgcggaatg gacctgcggg tacctgggtt tcgtaaatga cacggcccat
    46861 ttgggttaca gtcactttag cggtagtgtg tgcaacacct gaaatatcgg gggcgtagcc
    46921 gcgcaggttt ggcggtaaca tttgatcatc ggtactgatg ctgccgccaa tgtaactgaa
    46981 gccatcaaaa atatcggagt tgagataatc ttcgcccagc gaaagtttgg cttttaatga
    47041 cggcaaggcg cgccaggcgt agtaacgact ccactcccag tttttttgcg taccgctatc
    47101 gtcagtgtcg ccgtcatcgt tactgcgggt atgctgataa tcgctctgcc agtcagctcg
    47161 taagcgccac ggcccaacgt taacgccgac cgtaccatta ccgctgatgt cgttagtgtc
    47221 atcaccccca ttttcatgtc gggtttgcgc gttgatactg taatcggcaa taagcccagg
    47281 gataccgtca tcccagcgcg aaggcgggtc ccactcgctg tcggtatatt ccagataagc
    47341 ctgcggtaca gaaacaagga gtgcagactg gcctaaatcg ccgctaattt ctgtacctgc
    47401 taaaagtgcc gtattcagac actgaccatc gcggatccat tgcaaattct ttgcaatatc
    47461 ttccttcaga ccaaactggg caaccagctc tggcgataag caggcatatg atttattcgg
    47521 atcgttttca gttgcgtacc agtagatatc gtagtcgtca ggtaacggct gattatttac
    47581 atgaacgcgt aagttatatt ttcccggttc tacatagcct ttttgcgaaa aacggcccaa
    47641 atcaattttg gtattgcctt ttaaatcaag aaatcggcca tcaaactgaa tggtatcgtc
    47701 tgccagggca ttaaatggac ttccagaaat tgccagagca atccaccacg aaagcccccg
    47761 taaacggaga aaagaatgtt ctggcatcgg cgtatccatc catgattttt tataatttag
    47821 ttatttggta gttataaacc ccataacttt gcagctatgg gggaaataca ttagagatac
    47881 gtgatctgga acgtcgtcag cgtttcaaac ggtgccggtg ttggtgttac agcatccgtt
    47941 tcacccacta accatgcttt aaagttcaga gtttgtttag gcttgccttt tcccgttgaa
    48001 ctgtccttca cgataggttg cttaatctct gctcccagtg cataacctgt accgtgctgg
    48061 tcaccgataa ccaggctaac gtttggaatt tcagttccat tatccatgtt agacagagaa
    48121 tagttatctg cgttagtgga gtacggatta ccggtgaatg tggtagccat tgtcgtttct
    48181 gtattgaaat tacagtcctg taaacgaatc tggaaatctt ttggtgtggt tgttttgccg
    48241 ccggttaaag aacccgtacc gatgctgccc atatccactt ccagtttatg atcgtcagga
    48301 acaattgagc acggggagtc ttcaattgtg ccgtaaaatc tcactgtacc gttgttccct
    48361 gcggcaacga ctgatgctga agtcagcgcc cccatgagca ttgccgtggc aactgctgct
    48421 tttacaaact tactcattat ttgtccttaa atcataaata attagaatat aagccaactg
    48481 aaatattgac acagagcttc ttcaattttt acagcgactt ttaagcagcc aatgaacaga
    48541 ttgtcagctc ctgaaaaaac aggttccttg atataatatt ctttggcg
//
LOCUS       DACBVI010000017        47735 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.017, whole genome shotgun sequence.
ACCESSION   DACBVI010000017 DACBVI010000000
VERSION     DACBVI010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.017"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            109..1839
                     /locus_tag="IU325_04975"
     CDS             109..1839
                     /locus_tag="IU325_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HAP5808394.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRC
                     QLEDQLEYQTLKARAEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFSCQYAGLIAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNKPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPG
                     DRCVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPDAETRAVSTFVNCGKALP
                     EHGIEIRNEAGIPVAERVVGHICISGPSLMSGYFGDQISQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLHVQESLA"
     gene            1854..2750
                     /locus_tag="IU325_04980"
     CDS             1854..2750
                     /locus_tag="IU325_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP5808395.1"
                     /translation="MTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNLIWVRGS
                     LEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGFCQRFLF
                     ISSLAARHPELSWYANSKHVAEQRLTAMADEITLGIFRPTAVYGPGDKELKPLFDWML
                     RGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDWQRVRQL
                     AADARCGSVRMVGIPLSVLTCLADISTTLSRLAGKEPMLTRSKIRELTHADWSASNNR
                     ISEDINWFPGISLEHALRNGLF"
     gene            2782..3030
                     /locus_tag="IU325_04985"
     CDS             2782..3030
                     /locus_tag="IU325_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP5808396.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            3030..4202
                     /locus_tag="IU325_04990"
     CDS             3030..4202
                     /locus_tag="IU325_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP5808397.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIAEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     DGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(4237..5316)
                     /gene="lptG"
                     /locus_tag="IU325_04995"
     CDS             complement(4237..5316)
                     /gene="lptG"
                     /locus_tag="IU325_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAP5808398.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIFARSASIEDDKTWILHGVNHKKWLNGKETL
                     ETSDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(5313..6383)
                     /gene="lptF"
                     /locus_tag="IU325_05000"
     CDS             complement(5313..6383)
                     /gene="lptF"
                     /locus_tag="IU325_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAP5808399.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSVFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            complement(6414..6974)
                     /locus_tag="IU325_05005"
     CDS             complement(6414..6974)
                     /locus_tag="IU325_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAP5808400.1"
                     /translation="MSSRLIIALVIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     EDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTDYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWHLQEQSSTILVLNKTGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(6986..7810)
                     /locus_tag="IU325_05010"
     CDS             complement(6986..7810)
                     /locus_tag="IU325_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808401.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVNRQELLRLICKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRVYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKSRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPMALDSDIRQRLELLISRFPL"
     gene            complement(7810..9159)
                     /locus_tag="IU325_05015"
     CDS             complement(7810..9159)
                     /locus_tag="IU325_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP5808402.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYASNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVFSLIPTLKAFFQRHSLLYNSAGEKS"
     gene            complement(9205..9963)
                     /locus_tag="IU325_05020"
     CDS             complement(9205..9963)
                     /locus_tag="IU325_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808403.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKINQF
                     LAQRERQLYQQMAQYRPELIIRLGIDIDTAISRKPDHDYAELQDKIGVMSTIGYNGSK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(9995..10708)
                     /locus_tag="IU325_05025"
     CDS             complement(9995..10708)
                     /locus_tag="IU325_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808404.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFSRKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            10882..11574
                     /locus_tag="IU325_05030"
     CDS             10882..11574
                     /locus_tag="IU325_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAP5808405.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLDVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEKVLVDAEK
                     AITDFMTARGYH"
     gene            complement(11623..13122)
                     /gene="pitB"
                     /locus_tag="IU325_05035"
     CDS             complement(11623..13122)
                     /gene="pitB"
                     /locus_tag="IU325_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HAP5808406.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLITMEPPLPATSTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(13414..15273)
                     /locus_tag="IU325_05040"
     CDS             complement(13414..15273)
                     /locus_tag="IU325_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HAP5808407.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            15478..16344
                     /gene="yghU"
                     /locus_tag="IU325_05045"
     CDS             15478..16344
                     /gene="yghU"
                     /locus_tag="IU325_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HAP5808408.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTEAMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(16414..16662)
                     /gene="hybG"
                     /locus_tag="IU325_05050"
     CDS             complement(16414..16662)
                     /gene="hybG"
                     /locus_tag="IU325_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HAP5808409.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(16675..17016)
                     /gene="hypA"
                     /locus_tag="IU325_05055"
     CDS             complement(16675..17016)
                     /gene="hypA"
                     /locus_tag="IU325_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAP5808410.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEENAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(17009..17497)
                     /gene="hybE"
                     /locus_tag="IU325_05060"
     CDS             complement(17009..17497)
                     /gene="hybE"
                     /locus_tag="IU325_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HAP5808411.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(17490..17984)
                     /locus_tag="IU325_05065"
     CDS             complement(17490..17984)
                     /locus_tag="IU325_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HAP5808412.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     ATHD"
     gene            complement(17984..19687)
                     /gene="hybC"
                     /locus_tag="IU325_05070"
     CDS             complement(17984..19687)
                     /gene="hybC"
                     /locus_tag="IU325_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HAP5808413.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(19684..20862)
                     /gene="hybB"
                     /locus_tag="IU325_05075"
     CDS             complement(19684..20862)
                     /gene="hybB"
                     /locus_tag="IU325_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HAP5808414.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(20852..21838)
                     /gene="hybA"
                     /locus_tag="IU325_05080"
     CDS             complement(20852..21838)
                     /gene="hybA"
                     /locus_tag="IU325_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HAP5808415.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(21841..22959)
                     /gene="hybO"
                     /locus_tag="IU325_05085"
     CDS             complement(21841..22959)
                     /gene="hybO"
                     /locus_tag="IU325_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HAP5808416.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(23148..23435)
                     /gene="yghW"
                     /locus_tag="IU325_05090"
     CDS             complement(23148..23435)
                     /gene="yghW"
                     /locus_tag="IU325_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HAP5808417.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKYCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(23554..24441)
                     /locus_tag="IU325_05095"
     CDS             complement(23554..24441)
                     /locus_tag="IU325_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAP5808418.1"
                     /translation="MPRLTAKDFLQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHYAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            24598..25638
                     /locus_tag="IU325_05100"
     CDS             24598..25638
                     /locus_tag="IU325_05100"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP5808419.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLREWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(25678..26172)
                     /locus_tag="IU325_05105"
     CDS             complement(25678..26172)
                     /locus_tag="IU325_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HAP5808420.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            26363..27247
                     /gene="yghA"
                     /locus_tag="IU325_05110"
     CDS             26363..27247
                     /gene="yghA"
                     /locus_tag="IU325_05110"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAP5808421.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEQDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLATQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(27286..27711)
                     /gene="exbD"
                     /locus_tag="IU325_05115"
     CDS             complement(27286..27711)
                     /gene="exbD"
                     /locus_tag="IU325_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HAP5808422.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(27718..28452)
                     /locus_tag="IU325_05120"
     CDS             complement(27718..28452)
                     /locus_tag="IU325_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbB"
                     /protein_id="HAP5808423.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            28704..29891
                     /gene="metC"
                     /locus_tag="IU325_05125"
     CDS             28704..29891
                     /gene="metC"
                     /locus_tag="IU325_05125"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HAP5808424.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQEAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMVDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVNDLIADLDAGFA
                     RIV"
     gene            30031..30690
                     /gene="yghB"
                     /locus_tag="IU325_05130"
     CDS             30031..30690
                     /gene="yghB"
                     /locus_tag="IU325_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HAP5808425.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTILMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(30730..31629)
                     /locus_tag="IU325_05135"
     CDS             complement(30730..31629)
                     /locus_tag="IU325_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP5808426.1"
                     /translation="MKREEICRLLADKVNKLKNKENGLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            31823..32986
                     /locus_tag="IU325_05140"
     CDS             31823..32986
                     /locus_tag="IU325_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HAP5808427.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            33091..33918
                     /gene="dkgA"
                     /locus_tag="IU325_05145"
     CDS             33091..33918
                     /gene="dkgA"
                     /locus_tag="IU325_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HAP5808428.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            34118..35044
                     /locus_tag="IU325_05150"
     CDS             34118..35044
                     /locus_tag="IU325_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3828 domain-containing protein"
                     /protein_id="HAP5808429.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQPD
                     ADHADAVVRFRIFKDDKEKTMQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     AIASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFMTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTTDSARIHVRFTLTNGNNEEQELILQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            35095..35352
                     /gene="yqhH"
                     /locus_tag="IU325_05155"
     CDS             35095..35352
                     /gene="yqhH"
                     /locus_tag="IU325_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HAP5808430.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSDANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(35394..37613)
                     /locus_tag="IU325_05160"
     CDS             complement(35394..37613)
                     /locus_tag="IU325_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HAP5808431.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEESIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(37724..39136)
                     /gene="ftsP"
                     /locus_tag="IU325_05165"
     CDS             complement(37724..39136)
                     /gene="ftsP"
                     /locus_tag="IU325_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HAP5808432.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(39211..39948)
                     /gene="plsC"
                     /locus_tag="IU325_05170"
                     /gene_synonym="parF"
     CDS             complement(39211..39948)
                     /gene="plsC"
                     /locus_tag="IU325_05170"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HAP5808433.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIADLDKEVAEREAAGKV"
     gene            complement(40182..42440)
                     /gene="parC"
                     /locus_tag="IU325_05175"
     CDS             complement(40182..42440)
                     /gene="parC"
                     /locus_tag="IU325_05175"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001639862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP5808434.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDERRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPLSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(42578..44185)
                     /locus_tag="IU325_05180"
     CDS             complement(42578..44185)
                     /locus_tag="IU325_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP5808435.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAM
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALGNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(44294..44776)
                     /locus_tag="IU325_05185"
     CDS             complement(44294..44776)
                     /locus_tag="IU325_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP5808436.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTEQRLETDVYVPLA
                     "
     gene            complement(44829..45221)
                     /gene="ygiW"
                     /locus_tag="IU325_05190"
     CDS             complement(44829..45221)
                     /gene="ygiW"
                     /locus_tag="IU325_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HAP5808437.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            45373..46032
                     /gene="qseB"
                     /locus_tag="IU325_05195"
     CDS             45373..46032
                     /gene="qseB"
                     /locus_tag="IU325_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HAP5808438.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            46029..47378
                     /gene="qseC"
                     /locus_tag="IU325_05200"
     CDS             46029..47378
                     /gene="qseC"
                     /locus_tag="IU325_05200"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HAP5808439.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADSFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(47427..47708)
                     /locus_tag="IU325_05205"
     CDS             complement(47427..47708)
                     /locus_tag="IU325_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HAP5808440.1"
                     /translation="MLTYWLIDAFFAFNKYDWMLESGDNICSIPSVSGEDRILQAMIA
                     AFFLLTPLIILILRKLFMREMFEFWLYVFTLVICLVCGWWLFWGRFIFC"
BASE COUNT        11833 a        12641 c        11946 g        11315 t
ORIGIN      
        1 taaatcaatg ttcattttca cactgatgaa aatactatct catttatctt ttatgcctat
       61 ataatccacc gcattattgt cattttattt ttgatatcca attttttcgt ggtgtatatg
      121 tctaataaaa tctttacgca ttccctacct atgcgctatg ccgattttcc aacgctggtt
      181 gatgctttgg actacgccgc tctgagtagc gccggaatga atttttatga cagacgttgc
      241 caacttgaag atcaactgga atatcagacg ttaaaagcgc gtgccgaagc tggtgcgaag
      301 cggttgttat cgctgaacct gaaaaaaggc gatcgcgtgg cgctgattgc cgaaaccagt
      361 agcgggttcg tagaggcttt tttttcctgc cagtatgccg gcttaatcgc cgtcccgttg
      421 gcgattccaa tgggcgtcgg tcagcgggat tcctggagcg ccaaactgca gggtttactg
      481 gcaagttgcc agcccgcagc cattatcact ggtgatgagt ggttaccgct ggtcaatgcc
      541 gcgacgcata acaaacccga attacatgtt ttaagccacg cctggtttaa ggcattaccg
      601 gaagccgatg ttgcactcca gcgtccagtt ccgaacgata tcgcctacct ccagtacacc
      661 tccggcagca cccgttttcc ccgtggcgtc attatcaccc atcgcgaagt gatggctaat
      721 ctacgtgcta taagccacga tggcattaaa ttacgccctg gcgaccgctg cgtctcctgg
      781 ctgcctttct accatgatat gggactggtc ggctttctcc tgacccccgt cgccacgcag
      841 ctctcagtag attatttgcg cactcaggat tttgccatgc gtcctctgca atggcttaaa
      901 ttgatcagta aaaatcgcgg caccgtttcc gttgcgccgc cgtttggcta tgaattgtgc
      961 cagcgccgcg tgaatgaaaa agatctcgct gaactggatc tttcctgctg gcgcgtcgct
     1021 ggtattggcg cagaaccgat ctccgcagaa caactccatc aattcgctga atgttttcgt
     1081 caggttaact ttgacgataa aacgttcatg ccgtgctacg gactggcaga aaatgcgctg
     1141 gctgtcagct tctctgatga agcttccggg gttgtggtta acgaagtgga tcgcgacatc
     1201 ctcgaatatc agggcaaagc cgtcgcgccg gatgctgaaa cacgcgccgt atcgactttc
     1261 gtcaactgcg gtaaagcgtt gccggaacat ggcattgaaa tccgcaatga agcaggtata
     1321 cctgtcgcgg aacgtgtggt aggccatatt tgcatctccg gccctagcct gatgagcggt
     1381 tactttggcg accagatttc acaagacgag attgccgcga cgggctggtt agacaccggc
     1441 gacctcggtt atctgctgga cggttatctg tatgtcaccg gacgcattaa agatctgatt
     1501 attattcgtg gccgcaatat ctggccgcag gatattgaat atattgcgga acaggaaccg
     1561 gaaattcatt ctggcgatgc gattgctttt gttaccgccc aggaaaaaat cattttgcag
     1621 atccagtgtc ggatcagcga tgaagaacgt cgcgggcagc ttatccacgc gctggcagca
     1681 cggatccaaa gcgaatttgg cgtgaccgcg gctatcgatc tgttgccgcc ccacagtatt
     1741 ccccgaacgt cctccggcaa gcctgcccgt gcggaagcga aaaaacgtta tcagaaggct
     1801 tatgctgcaa gccttcatgt gcaggaatcc ctggcatgaa tcaaactgtc gcggtgacgg
     1861 gcgctaccgg gtttatcggt aaatatatta tcgataacct gctcgcccgc ggctttcacg
     1921 ttcgcgcatt gacgcgtact gcccgcgctc acgtcaatga taatctcatc tgggtgcgcg
     1981 gttcgctgga agatacacat tcgcttagcg aactggttgc cggagccagc gcggtggtcc
     2041 attgcgccgg acaagtgcgc ggacacaaag aagagatttt tacccgctgt aacgttgacg
     2101 gcagcctgcg cctgatgcaa gcggcaaaag agagcggctt ttgccaacgt tttctgttta
     2161 tctcttcgct ggcggcgcgt catcccgaac tctcctggta tgcaaactcc aaacacgtcg
     2221 ccgaacaacg actgaccgca atggctgacg aaattacgct gggcattttt cgcccgacag
     2281 ccgtgtatgg tcccggcgat aaagagttaa aaccgctgtt tgactggatg ctgcgcggcc
     2341 tgctgccacg acttggtgcg ccagatacac agctctcttt cctgcacgtc accgatttcg
     2401 cgcaagccgt ggggcagtgg ttaagcgccg aaactgtaca gacgcaaacc tatgaattat
     2461 gcgatggcgt cgccggcggc tatgactggc aacgcgtaag acagcttgcc gccgacgccc
     2521 gttgtggttc cgttcgaatg gttggtattc ctctgtcggt actcacctgc cttgcggata
     2581 tcagtaccac gttgagtcgc ctggcaggta aagaacctat gctgacccgc tcgaaaatcc
     2641 gtgaattaac ccacgccgac tggtcagcaa gtaataaccg tatttctgaa gatattaatt
     2701 ggtttcccgg gattagcctg gaacacgcat tacgcaacgg gctattttga ttgtcttgtt
     2761 taaaggtgtg ttaacgtttc tatggtaaat cgtgaaatag taatggatta catcctttcc
     2821 tgtttacagg atttggtcga aaacggtgtg gaaattaagc ccgacagcga tctcgtcaac
     2881 gatcttggcc tggagtcgat caaagtgatg gatctcttaa tgatgctgga agacagattc
     2941 gatatttcca ttcctattaa tattctgctg gatgtcaaaa cccctgcgca attaatggaa
     3001 acactacttc catggctgga gaataaataa tggggctata cgataaatat gcgcgtcttg
     3061 ctggcgagcg actgcaattt agtgacaacg ggttaacgcc gtttggtacc tgtattgatg
     3121 aagtgtattc cgccaccgaa ggccggatcg gcaataaaaa agtgattctg gccgggacca
     3181 ataattatct cggattaacc ttcaatcatg atgccatcgc cgaaggtcag gcagcgttag
     3241 cggcccaagg caccggcacc accggttctc gtatggcaaa cggcagttat gcgccgcatc
     3301 tggcgttaga gaaagagatc gccgagtttt ttaatcgccc taccgctatc gttttttcaa
     3361 ccggttatac cgctaattta ggcgtcatca gcgcacttgc cgaccataac gccgtggtgt
     3421 tactggatgc cgacagccac gccagtattt atgacgcctg ttctctcggt ggcgcggaaa
     3481 ttattcgttt ccgccataac gatgctaaag atctggaacg gcgcatggtt cgcctcggtg
     3541 aacgggcaaa agaggcgatt atcatcgttg aaggcatcta cagtatgctc ggtgacgttg
     3601 cgccgctggc ggaaattgtt gatatcaaac gtcgtctcgg tggttatttg attgtcgatg
     3661 aagcgcattc gtttggcgtg ttaggcgcaa cggggcgcgg actggctgaa gccgttggcg
     3721 ttgaagatga tgtggatatt atcgttggta ctttcagtaa aagtctggcc tcgattggcg
     3781 gttttgctgt cggttcggaa gcgatggaag tgctgcgcta cggcagccgc ccgtatattt
     3841 ttaccgcctc tccttctccg tcctgcattg cgaccgtgcg ctcgtcgtta agaaccattg
     3901 ctacacaacc agagttgcgg caaaagctga tggataatgc caaccatctg tatgacggtc
     3961 tgcaaaaact gggttacgag ttaagttcac atattagccc ggtagtgcca gtaataatcg
     4021 gttcgaaaga ggacggttta cgcatctggc gcgagcttat ttcgctgggc gtttatgtga
     4081 atcttatcct gccacctgcc gcgcctgcag gcatcacgct gctgcgctgt agcgtaaacg
     4141 ctgcccacag tcgtgagcaa attgatgcca tcattcaagc gttcgctact ctgaagcagt
     4201 aatgtgagcc cgctgtggcg ctgcgccacg gcgggtttaa tgttgtctgt cataccaata
     4261 caccaggctc aacgccacca gcacaaacgc taccggtaaa ccgagcgcgg taacaggtgc
     4321 gctcaacgca aatagcaacc ccagattgac catgatttga tagctgatcc aggtgagtaa
     4381 gccaacgatg acgcctacag cgagacggct acccatccct ggcgaacgcg gggcactaaa
     4441 ggtaaacggc acagccagca aaatcatcgc cagggtcagg atgggttgtc ccagtttttc
     4501 ccacagtgca aggcggtatt cgctgctggg ttgcccagtt tcctgcaaat aatggatgta
     4561 atgattaagt tgacggacag aaaaagtatt tcccggcatc gataactctt caagattcat
     4621 actggtgaag gccgattgcc aggcaagatt atctgatgtt tccagcgttt ctttgccatt
     4681 caaccatttt ttatgattca caccatgcag gatccatgtt ttgtcgtctt caatggaggc
     4741 actgcgtgca aaaatgtagg attcgagcga gagatcgtcg cgataatgaa atatctccac
     4801 gcccacaggc tggccctgct cgttcagcga tttcaccgtc acaaattcat taccgcgcct
     4861 ggcccaaagc atattgccgg taatgtcatc gtcctccccc aacgcggtgg cagtcgattt
     4921 gatttgcagc gcctgttgct gcaatggcga cgccacccac tcatctatcg cgcctaacga
     4981 aacagtccac aatatcccgg caaccagcgc caccagtgca atacggaaga tagaaaaccc
     5041 agtgctgcga atggcggtaa gctcactgtt tttcgataac tgccccaggc caacaatccc
     5101 tcccaataag gcgataaacg gcgaaagttc gaccagtgta cgcggtaagg tcatcagcac
     5161 caccagcacc gcctgagtcc agcgataacc gcccgggctg acatcatcca gttcgttaat
     5221 caggttgaag gtagtaaaaa gcggcagcaa tagccctgcg gcggcggcaa aaccgagaaa
     5281 gagatgacgg attaaatagc gactgaaaac attcatcggg aaaatttctg cagcaaagag
     5341 aagtcgcgtg cgaccagcat cagcaggccc attagcatga gtccgggtac taaccacaaa
     5401 ccaggaatga ggggaatcgc gccgttagcc accagcgtac ggcagacgtc gccgccataa
     5461 aaaatggcaa caaacagcaa cgtcagtggc aataacgttg aaaatcgccc ttgccgcggt
     5521 tttacccgac ttaatgaaat ggccagcagc gccatcaaca acgctgtcag gccacggctt
     5581 tgtcgccatt gcagttcggc atgatcggct ggaaagacgg agcgcgccag ctccgtgacc
     5641 gattttgctt tgcgtttgac gttagggctt tgatccagcg gattcagatg taattgcaga
     5701 ttacggtaga tctgttcgtt gtcgtcacgc ccctgatgat cgagaagata ggcggtcccg
     5761 ttatgcaaca taacggtcgg tttctcaggt gatgggtcaa ccacatcaac cgaacgggcg
     5821 cggaaaatgc gggttcgatt ggcagtagaa gtataaatca gcgcgtcagt caggcgatta
     5881 ttatcctgat caaccgtctg cgaaaggatc attcgtccgt tatcgttagt gttaaatttc
     5941 tttgcccgca actggcgaac atccagctcc gactgtgact gttgctccag ttgataaatc
     6001 tgcgcataag cccacggacg accatacatc gacagaaggg tgacaaaaat acttagcggt
     6061 atcgccagat aaagaacggc tttgtacaag cgtcccggac tgccccccgc cgcagagata
     6121 gcggtaattt ccgagtcggt atacatctgc cctagcgtca cgccaactga cacatacagt
     6181 ccaacaggta acaacatctc cagtgcaatc agcaccttgt agaaaacgat atccagcaca
     6241 acatcaagag ctaatgtccc atttgccgct tcggtcagat aacgctgtgc ggagtagctg
     6301 gcgaaaataa agatcagaaa accgacgata atcagcacca gacggcgcgt accacgcatg
     6361 atgtagtgtt caaccagctt catctcacta acgccctcaa tcctttgaat tttttattca
     6421 ttgatcagtt tctgcgccag cgcaatgaca tgatctacct cttccggcgt cagactgccg
     6481 tcctttgccc actgaatttg cccggtttta ttcagcacca gaatggttga actctgctcc
     6541 tgcaagtgcc aggcaacgcg tccctgtcca ttgccgtcaa taacgaattg cgcccatgga
     6601 tagcggcgtt tatttttttc gattttgcca acgacaaaat agtcggtacc aaagatggca
     6661 tcatcagtgt tcacaatggt ggtgggctga aaacgatcct gcggaaagtt tgccgcttca
     6721 accgctttaa ttaacagtga gttctttttc ttcgccgact tacgcccggc gatatactga
     6781 ataatgcgga cttttcccgc cagctgtgtg ctgttccact tttggtagtg aatttcgtct
     6841 tcgctattca gaagcagctc gccgccttcc tggatataaa caggggtaac cgttttacca
     6901 gtgacgaaat tgtgcgcctg cacacccggg gctaagagca tgataaccag cgcaataatc
     6961 aaacgagatg acatcattgc tccggttata gcggaaaacg cgaaatcagc agttctaagc
     7021 gctgacgaat atcgctatcc agcgccatcg gtgcatcata cgactggcgg ttttccatat
     7081 catccgccac catgtcgtcg tgtttatccc ccatcaggcc attaatggcg gcgaggaagc
     7141 gggaaatctg taacgcctct ttattttcag gatcaagcga tcttagggtg tcgtaataag
     7201 aaacctgctg gctgatgatt tttcgcacat catgaaattc cctgccagtt agtcgcgatt
     7261 tggcaattat ctctttcagg gtatgcattt gcttttgccg ataggcaata aaaccgctta
     7321 cgctttctaa ctggctatgg cgtaatgaat agttcaccaa cgaccagacc ggagagctta
     7381 aataaacccg taacaggttg ccgtaatacg acacaatatt tttcttaccg ttacgaaagc
     7441 cgtcctgaaa acgccccaaa aaaacggtcg ttttgccgaa taaaaatccg gcctgatgct
     7501 tttttaaata cagccgttgc gcaaaacgca ggtgcttgta gcgcgctcgc atgtatttaa
     7561 attgtttgcg ctcatcagca gtcagctcat cgcctttggc ctgtttgcat attaaacgca
     7621 gtaactcctg gcgattgacg ccattgagcc agaattgcag gcataacgcg taattatcat
     7681 ggatgatttc ggtggtacaa cttagtgcga tagtcggcgg caaactgacc tgtgcatcaa
     7741 caacatcatc ttcttcaatc gcaacaaata gacgctcgct cgcctcaagc gggaaatatt
     7801 ttctcatcat caggatttct ctccggctga gttataaagc aaggaatggc gctgaaaaaa
     7861 tgcctttaag gtcggaatta gcgagaacac cacatccaga cattgcccac cgaagtaagc
     7921 aaatgcagcc cccagcacgc caaacagatg acagaacatg aacagcaata cgatgtagcc
     7981 gattgaggcg atggtttgcg ccacgagaaa agcgcgctgt tttcccgcca tcaatagcaa
     8041 tgactcctga gggaagccca gcatcgagat cacaattgcg cccagcatca cctgaatcag
     8101 gtcatacgct tcgagatact taacgccaaa caccagcgaa atgagcggct tgccgacgat
     8161 caacaccgcg agcgccacca gaataccgat tccgcccgcc agtaaaccgg acttcacgcc
     8221 taacaaccac ggtctggtgg tgcgcggatc taaacgcatc acctctgggt aaaaactttt
     8281 acccagtaaa cccgctggcg ttccggcggc gtcgaagaat gtcatggcga ttttaaataa
     8341 cccggcggca gcgggtccta acacgatccc caccaacact gtgctgcacg aattacgcgc
     8401 cgaccagatg gaatgggcaa tgttggttga ccagacaaaa ctccacgcgc ctttgatgtg
     8461 tcgggcagac tcaaacagat tcaatttgaa cgcgttatgg atatttctgc ggcgtaattc
     8521 acgtgcggca aaccaccagt acatggtgcc gccaacaaga ttcgaggcgt accaggcaat
     8581 aacaaaaccc gcaaaaccaa agtcaaaata ccaggcaacg acgctccccg ctgcgcgcag
     8641 aaaaggtttc gtcgcctgct gtacagcaat taagtcgaag cgatctaccg cacgcagaat
     8701 gccggtcggc gtggaggaag ccattgaagg aatgagcgtg caatacagcg ctgccagcca
     8761 aaagctttgg tcatccagac ctaatgaatg ggaaagaaaa gggagtaagg caatgccgcc
     8821 gacaatcgcc accgcgccgc tgacgatatc taacgagaag gaaaatgaga cgacattgcg
     8881 aaattgctgc ggattattgt tggtcaaggc tggcgttccg tactgcacca ccaactgcca
     8941 tgtctgaaac ttaataaaat cgctgatcga cttggcgtac gattgcacaa tcaccagtac
     9001 gccaaacatg gcgggcgtca tccctttacc ggcacacgag agcgccaaca gacccagcaa
     9061 agcgctcacg acattactgg agcctaacca ggcgctattg cgaataatgg tgcgaaacgc
     9121 gccatctgca aaccaatgtt tgatgttaaa acccgccaat tcagcctgac cttatcgttg
     9181 atagtaaaaa gtatcccgcc agccttaagt taaacttcgg cggtcagaaa cgatggcaac
     9241 cagagaaacc gccttctgtg cctgttccag cacttcgctg tagggcgctc tggaatcaat
     9301 ctcaagaatt tttgagccgt tatagccaat cgtcgacatg acgccgattt tgtcctgcag
     9361 ctcggcatag tcatggtcag gcttgcggga gatggcagta tcaatatcaa tccccaaacg
     9421 aatgattaat tcaggacgat actgcgccat ttgttggtat aactgtcgct cgcgctgcgc
     9481 cagaaattgg ttgattttcc cggtcgcgcg ctcgacgcca atccccggtc cgtcataata
     9541 aaagcccgaa atttcagcct gcgggaagcg atcgctgacc accagaacgc cactttgcgc
     9601 cagtcgctga accttgcgta gattcgccat tcttcgcagc gagaagcagt acataatcac
     9661 cgccgcccat agcgctggag atttggtttt catgctttgg gttttcgagg atttggccgc
     9721 cagtcgccgt tccagccaga cgccaaccaa cggcaatcgt ttgattttgt cgccgtcttc
     9781 gccggagagc agccccaaat agcggcgctc ggtttgccag tgttgttgca gcgatttcac
     9841 caggtcggtg gtcagcgtgg atttaccggt gccatcacac ccgaccaccg caatcagccc
     9901 cggaatgtag ttgggctgcg gcgcggttgt actattaaca gtttgtgttt gtagtgcatc
     9961 cattcgttaa atcatcccct atccattttg gtgatcagga aagcgcggcg tgaattgcgc
    10021 gtagtgacgc ttgcataatt tcatcggctg gttgccgacc atccagttca aggatctttg
    10081 cgccattgaa tgtcagttgc ggcgtaacgg cgattttttc ctgtaacgcc gccagttgat
    10141 ggtcaggttt acgcgaaaac gcggtttgtt catcaatgcc aagacgaatc aacaatacgg
    10201 gcaaataaga cgccatccat tggtacagct tcagctcgcg ctgccttaac atttttatcc
    10261 agccgttacc gcccgtggtt tttgccaatt gcgggccatc aaagcgaaaa cccggcactt
    10321 caacctgcgg gtagcggtcg gtgatgagca gatagccttg ctggctttta cacaacattt
    10381 tgcgaaactt gtacgcccgc cagcaggaaa gcagatagat aaccagcgca gtaatattgc
    10441 caggcggtgt taagggcttt tcgtgcacat gtgccgcttt acttcgcaaa taacgcccaa
    10501 aaggtgcgcc aataacaggg agctgtgaaa tccattcgcc aattcgcccg gacgattgcc
    10561 cgagataaat gtgttctgtt ggcattcttg ctgccagttc atttaccagg cttgccgtga
    10621 gggtcgattt acctgaacca tcacatccta caatggcaat aacacgcact ggcgtggaat
    10681 taattattga catattacgt tgattcacga aaaacccggc agcaaataat gtattgaata
    10741 ttacgtggtc cgtgctcaga atatccgttc aaccttattg tgacaatggt taattgtgac
    10801 attgacctga attacgcttt cataaaaaca tattaaccaa ataaatattt ttaatggata
    10861 tttaaattaa aggatatatt catgcagtca ataacacctc cattaattgc cgttattggt
    10921 agcgatggtt caggcaagtc aacggtgtgt gaacatctca ttaccgttgt cgaaaaatat
    10981 ggtgcagccg aaagagttca tttaggaaaa caggccggaa atgtcggtcg tgcagtgaca
    11041 aaattaccgt tgatgggaaa atccttacat aaaacaattg aacgaaatca ggtgaaaaca
    11101 gcaaaaaaat tgcctggccc tgttccggcg ctggtaatta cagcgtttgt cgcccgtcgc
    11161 ttactgcgct ttcgtcatat gcttgcctgt cgtcgtcgcg ggttaattgt tctaaccgac
    11221 cgttatcctc aggaccaaat tcctggcgct tacgatggta cagtgttccc acctaacgtt
    11281 gaaggcggtc gttttgtctc atggctggca agccaggaac gtaaagcgtt tcactggatg
    11341 gcgagccata aacctgatct ggtcatcaaa ctcaatgtcg atctagatgt ggcctgcgcg
    11401 cgtaagcctg accacaaaag ggaatcgctg gcaagaaaga tagccataac gccacagtta
    11461 acctttggtg gtgcacaact ggttgatatc gacgccaatc agccactgga aaaggtactg
    11521 gttgatgcag aaaaagcgat tacggatttt atgaccgcgc gtggttatca ctagtcaaaa
    11581 atggaaatgc ccgatcgcca ggaccgggca ttttcaggaa gattaaatca actgcaatgc
    11641 tatccagtac aacccaccag aaagaaaaat cgccgccggt aaagtaaaca cccacgccat
    11701 caggatgctg gttaccgttt tacgctgtaa cccaccgccg tccaccacca tcgtccctgc
    11761 taccgcagac gagaggacgt gtgttgtgga gacgggcatc ccaatataac tggcaaggcc
    11821 gatagatacc gccgccgtca tctgcgccgc catcccttgc gcatacgtca tgccgcgctt
    11881 accaatcttc tcaccgatgg tcatcgctac acgacgccag ccaatcatgg taccaatgcc
    11941 gagcgccagt gctaccgcca tgatgatcca caccggagcg tactcaatgg tgcttaacat
    12001 atcgctgcgc aattttttca gcaggttctg atcttcttta ctgacacctg gcagtttcgc
    12061 cagcttcgca gaggtatcag agatgcacag cataatgcgg cgcagctggc tgcgctgact
    12121 cacgcttaac ggctcgtagc tttccatatt gcctggcagc atcgttttaa cgcgcgcaat
    12181 cgcatcaaag gtatttgccg gatgacagtg aaactctgtt acttgcgtgc catcagtcga
    12241 tgtcgcaggc aatggaggtt ccatcgtaat caacttctgc ggcagttcag gatgctgttg
    12301 caggtagtgt tcgaagttgg tgacggcatc gcgggtacgg gtaatttcat agccggacgc
    12361 gttcatattg acgacgaagc cagcaggggc aatccccacc agtaccagca tcaccaggcc
    12421 gatccctttc tgcccgtcgt tagcaccgtg cgaaaacgcc acgcccgcag cggaaacaat
    12481 cagcgcaata cgcgtccaga atggtggctt acgtttgcct tttttctttt tgcgatcttc
    12541 cggaatgcgg tgaatacggt cacgcttttt cgtcccgctc cagtagcggc gcagcaggaa
    12601 tatcaggcct cccgcaatga ccaggccgac gataggggaa acaatcagcg aggagaaaat
    12661 tttggtcact tcacgcaggt ttaacgcatc catcaccgat gagccggtta acagcgcgtt
    12721 ggttaaaccg atgccgataa tcgcaccaat caaggtatgc gaactggagg ccggtaaacc
    12781 aaagaaccac gttcccaggt tccagataat cgccgccagc agcatggaaa agaccatcgc
    12841 caggccgtga gttgacccca tattcagcaa caaatcggtt ggcaacatat ggacaatggc
    12901 ataggcaacg ctaagtccgc ctagcaatac gccaaaaaag ttaaaaaatg ccgccatcac
    12961 cacagcaagt tgtggttgca tggcacgagt ataaataacg gttgccaccg cattcgccgt
    13021 atcatgaaaa ccattgattg cttcgtagaa caacacaaat gccagagcaa gtaataacaa
    13081 aagccccgtg tatatatcaa ggccaacaaa taaatttagc atatattaga ttacgccatt
    13141 ttgaatttac gaacggacgc attatcagtg actttaacgg cgtgggcaaa gtgaaatatc
    13201 attttttaac gataaaaaga gtgttatttc ttgtgtatca gataataagc attcattcaa
    13261 aacctttaga aataatcaaa acacaggtat tccactggtt aatatattca tatattaaat
    13321 aaataaatat tggaatatat aaatgttgaa tgttttgatt aatccccgta ctgattattc
    13381 ttcataatca gtacggggtt gctacaacat gtattactct ttcaccacaa ttagcggttc
    13441 aatatcactc tcttttttga tgaccagtga ttcatcaccg cgcaaacacg tcccaccgta
    13501 gttgccgcca acggtgaagg tacatacctg aatgtattta ccgtccactt tcggcaaaca
    13561 ccacagttgc tgatagatgt ttttctgctc ggcaaattta ccgctggttt tgtccagcac
    13621 ctcttcatga tggctgacga ggtcgatgtt actgccacag cgaccggcga tcggtttcac
    13681 tgcgtaacct gtttttacca gttcatcatt aacagtgaaa tcggtatcca gtaggtaacg
    13741 atggtgcggg aacagcgacc agaggatcgg cagaatcgct ttgttgccgg ggatcactgt
    13801 ccacagaggt tcaaagacca gcacttccgg gcgcagcaat acgtcgataa gacgcacttc
    13861 attttgcgga tgaccggtac ggatcggcac cgcagcaaac tcacggtcgc taacttcacg
    13921 aatctgatca aacgcggttt cccacgccca ggttttccac acgcagttaa ccagtcgccc
    13981 ttccccatca atcagttgcc cggcagcatc ccagcccagt tcatccagcc cgcgcaagat
    14041 acgcgtttca aagcccgcct ggtgcagcgc ctgctccata aactgcgcgt gatagttttc
    14101 ctcgatatct ttgtcttgca tgatatggac aaacggacgt gcacgactgt gtttccaggc
    14161 accggccagt tcgttaatca gcccttccgc cggattgaag ccgttgcctt tatagccctg
    14221 ctccgcccaa cgttcgagga tcaagcccgc ttcggtatga caggaggcgg aatcggcgtt
    14281 gtattcgtag accttcaggc cacgttcgtc catacagaaa tccatacgac cggtgatcat
    14341 atggtgacgg cgacgctgcc aggagagacg caaacgtggc cagaggattt ttgggatgtc
    14401 gaacagcgcc agcaggttgt catctttcag caccttgtcg gtggcgtgaa gatacatcag
    14461 gtgcagctcg ttggtggctt taatcagctc ctgctcggca ctctcggtaa tggtgtagta
    14521 atgatacgga tcctgattga tcacctgacc gttggcctgc acataggcgt tttgtagcgg
    14581 atctttttca tccagccatt taccgtcaaa ctggcctttg ttttccaggc gcgctccgct
    14641 gattttcagc aactcgcctg caatttccgg ctgcggtaag ctgtattcag tatcttccgt
    14701 ctggatcatc cagccgagaa tggtggtgtc atcaaacgtg tctttaaggg tatagcagcc
    14761 gttttcgacc accatctcca gctcgcgcgt ccactgttgc ccttgcggta acggggaatg
    14821 aatcacgttc tgttccgcaa tacggacttt gttgccatgc aattgggtaa tgatggcgac
    14881 atggccagtg tctttaaatt caccgccttt atcccagata agaagcgcgc ccgcgaccgg
    14941 tgcacgcggc gagccattag gaaatgcctg caatggcagg atgttgtcat taaccacttc
    15001 acgcaggaag cgcagcgaga aaatctccca cgccataccc acgtcagtaa agaccacacc
    15061 gtaattcaga aagagaaaac ggcgagcaaa ttcaacgcat tgccatttgt ggcccatata
    15121 ttcgtcgtcg atatagctac ggaatacggc gtcatcttcg tattcctgcg gatcgagaga
    15181 actgtaatct gaagagtaga ttgctacacc acctggggcg tagcccaata atgtcccgaa
    15241 cggggcatcc tggctggtcg ttcctttgct catcacttta cctttaacaa tacaacctaa
    15301 gcagttggcg taattgttgt gctctgatta cctgctcagc gacattaacc ggacagaggt
    15361 cagactaatc atacacctca tcgcagcggc tggcgcaaag ggttaaaaaa ttcacattct
    15421 gtacagcaag tgacctgcta cactgcttca acactaccac tcagaaggca actcactatg
    15481 acagacaata cttatcagcc cgcgaaagtc tggacgtggg ataaatccgc tggcggcgcg
    15541 ttcgccaata tcaatcgccc ggtttctggt ccgacgcatg aaaaaacgct gcccgttggc
    15601 aaacatccat tgcaacttta ttcactggga acgccgaacg gtcagaaagt aacgattatg
    15661 cttgaggagc tgctggcgct gggcgttacg ggtgcagagt acgacgcctg gctgatacgc
    15721 ataggcgatg gcgatcaatt ctccagcggt tttgtcgaag taaacccaaa ctcgaagatc
    15781 ccggcgctgc gcgatcatac gcataatccg ccgatccgcg tgtttgagtc tggatcgatt
    15841 ctgctttatc tggcggagaa atttggctac ttcctgccgc aggatctggc aaagcgtact
    15901 gaagcaatga actggttgtt ctggttacag ggtgctgcgc cgtttctcgg cggtggtttt
    15961 ggtcactttt accattacgc gccggtgaaa attgagtacg ccatcaatcg ctttaccatg
    16021 gaagccaagc gcctgctcga cgtgctggat aagcaactgg cgcagcataa gtttgttgcg
    16081 ggcgatgagt acaccattgc ggatatggcg atttggccgt ggtttggcaa cgtggtgtta
    16141 ggcggcgtgt atgatgccgc agagtttctt gatgcaggta gttataaaca cgtacaacgc
    16201 tgggcgaaag aagtgggcga acgtccggcg gtgaagcgtg ggcgtattgt taaccgcacc
    16261 aacggaccgc tgaacgagca gttgcatgaa cgccatgacg ccagtgattt cgagacgaat
    16321 acggaagata agcgtcaggg gtaagtcgca acattggcag gcttatcgct atgtagccgg
    16381 ataaagcatt tacgccgcat ccggctttaa tcatcacgcg ctggtaatgt cgtaatccat
    16441 ttggcgcagc gcgtctaatg tggctttggc ttcatcttcg tcgatgatgc tcatggcaaa
    16501 tccgacgtgt accagcaccc actggcccag cagatcggca gggttacctt cacaaatcag
    16561 ggcgatattc acgtcgcgct tgataccaca tacttcaacc tgcgcaagct ggtgaatatc
    16621 ctcaccgaca gccagcacct ggcctggaac gccaatacac attgttaact ccggttattc
    16681 aacttcaata cttttgacga tcagcgaatc gccggtatcg acacgcaacc gctcgccatg
    16741 acagagcgga cactgcgcat cgtgctgatg aatctccacc acctggctgc aatcccagca
    16801 ccaggcctgg gcgggtttat agacgatatg taaatcgcac ccttgcgcca ccgttccgtg
    16861 gcagacaatt tcaaaactaa aacggacggc gttctcctca acgcaggaga gcgcgccaat
    16921 ttccagccac acggcggtga cgcgcttaac atcgtgctgc tccgcctgcc gttggataat
    16981 ttcaacggcg ctctggcaaa gcgacaactc atgcattttc gccactcctg cggccaaaca
    17041 gcagggcgcg acgccctgcg tgtggtacat ccggattcgt gactggcagc gaaaggatca
    17101 ttcgtgcgca gtcatccgtc aggcgttggc cctcttcaat cgacatgctg tgcgaaagcg
    17161 gcgacatcag cgagcaggag aggtattgcg aaacaccgtc cagttcgcca acggtaaagg
    17221 tcatggtgcc atacggcagt tgcagaccaa ttttttcact cactttgcgc agcggccaga
    17281 gttgatccgg gccggggaag ataactgcac tcagcatcca cggggtgatc acacaccccg
    17341 tccactgacc ttcgaacagc gtaaagtcag aaacatacac cggcattgaa ggatgcagaa
    17401 aagagagatc atgcatcgaa cgccgggcaa tttcttcaaa agctgcctgt acttgcgcct
    17461 tcggggaggt ctggaaacct gctatctctt cagtcatgag tcgcctcccg tgggatggct
    17521 tccacgcctg attcgcgcag cgcagccaga acctgctcaa gcgcaggttc aatcattgct
    17581 tcaaccgtcg gcgttaagcc gatgtgtggc tccagcgatt ccgggatcac gccgaccagg
    17641 gtcagctttt tcggaaactc accggtgaag cgcagggccg acaatacgtc ggccaggcca
    17701 agctgatgcg gagagatttt gttggtaaac aacgccggaa cttcttcatc ccgcaggatc
    17761 atcatcgttc ccggcgcgct cttcttcgac acaatggcat ccgcaatgat cagatgatcg
    17821 cgatttgcca tgtcgccaag cagctccatt cccgccgtgc cgccatcgag gatctcaaca
    17881 taatccggca gaatgtatcg ttgctctaac gcttcgacaa tccgcacacc gatggcttca
    17941 tcggtcagca aaatattgcc aacccctaag actaaaatac gcattacaga accttcactg
    18001 aaaccacttc gttgccgtcg gcatccacta cgtgtaccgc acaggccatg cacgggtcga
    18061 aggagtgaat ggtacgcacc acttccagcg gtttattcgg atcggcaacc ggtgtaccca
    18121 ccagcgactg ctcgtaagga ccgacgtcat cattgaagtt acgtgggcca gagttccagg
    18181 ttgatggaac aactgcctgg tagttgctga tgataccgtc tttaataacc atccagtgag
    18241 agagcatacc gcgcggtgct tcgaggaagc caacaccttt gaactcaccc gttgccggaa
    18301 tgttcggctt cacaaaggtg gtgtgatcgc ctttgccgat attggtgatc agtgcactgt
    18361 attgattttg caggatatcc tgcaattcgc agcagtgaac ggtacgacca ataatacggc
    18421 ccagcgtgga gtgcagctgc gccacttcca gcgtgttgcc agtcagtttc tgataaatcg
    18481 caacgatttc attcagttta ttctgggtag attcgcgacc tgccgccagt ttcaccagca
    18541 tattagccag cggacccact tctaccgttt tgccgtagaa agtcggtgat ttcacccagg
    18601 aatatttccc gtcgtcagac caaccatcat aagccggaat ggtggtgcct tcccacggtg
    18661 cctgcggcgc ttcgtcttta taccaggagt gcttcgcgct ttcctgaatg cctttgatca
    18721 ggtattcatc ggaatgagaa gtgatcggac gatacgagga cagatccgca ttctcaatgt
    18781 agccgcccgg gaacaggaag ctgccgtttt tactgtcggt cgggaattcc ggcacgctca
    18841 ggtagttcac cgcaccttta ccgcgcgtca gccattccgg atagaacgcg gcgataaccg
    18901 cggtatcaac cttataaacc tgctcaacaa agtcgctcag cttgtcgatg aaggacttga
    18961 tgtacatcag acgctcaaga ttcagcacgc ccagaccgtc gaggttgatc gggttagcga
    19021 ccccacctac tgccaggttc tgaatgtgcg gcgttttacc gcccagcagc gccacgacgc
    19081 ggttagcgtc acgctggcac tccagcgcct gcaggtagtg cgctaccgca atcaggttca
    19141 cttccggcgg cagtttcatc gccgggtgac cccagtagcc attggcgaaa atacccaact
    19201 gaccgctggc aaccagatct ttgatcttgt tctgaacttt agtgaactct tccggactgt
    19261 tcagatgcca ggtcgaaacg cctttcagca tttcggaggc tttggttggg tcagcttgca
    19321 gtgcagaagt gatatccacc cagtccagcg ccgaaagttg atagaaatga acgatatggt
    19381 catgcgtggt gtgcgcagcc agaatgatgt tacggatgta ttgcgcgtta accggaacgt
    19441 cgatattcag cgcactctct gccgcacgaa cggaagacag cgcgtgagtg gtagtacata
    19501 cgccacagat acgttgcaca atcatccatg catcgcgcgg atcgcggttt ttcacgatct
    19561 cttccatgcc gcgccacatg gtaccggaag cccatgcttt cgaaacgaca ccattttcga
    19621 tttcgcaatc gatgcgtaaa tgcccctcaa tacgggttac cggatcaata gtaattctct
    19681 ggctcatgct ttgctcgcct catgacgatt atgatcgttt tgttttaaag gaggaagtat
    19741 cggcagtaga cgaatgagta cgatgtaagc gcaaatctca atagccacaa aaccaataga
    19801 aatcaacagt tcttcccagg tcgggaagta ggcgtaaccg ccgcccgggt tgaatgccac
    19861 cagcgaatag gtcagacgcc aggttgcaca acctaacagt gcgctcagtg ctgacaggaa
    19921 cagcatgcgg gaatcattac gcagcttcgc cacacgcaga acgaccagcg ggaagagcat
    19981 cagcaggact tcaatccaga acatcacgga gtagaagtca ccggcaaacg ctaacgacag
    20041 cttgtcgcga tagataagct cgccaaagcg cagcacgatg aaaatcgcca gcaacacact
    20101 gatggtgttg gtcagcttaa cgaacagact cttttcatcc ggaccgttgc cacgcagacc
    20161 cgcctgcacc agcgaacctt caaagatgac aatcgagaag cccatgatga acgccgtcag
    20221 cagtgagaac agcggcaaca tttcatagct ctgccacagc ggatgcactt tgtagcccgc
    20281 cgagatcatc agcgacccca ttgaagactg gtgcatagtt ggcagcagcg caccgagcgc
    20341 gatgatgaag aacatcacct tgttcagccg tttgagcgac actttccagc ccagacgttc
    20401 aaacagtgcc ggagcaaact ccagtgccat cacgccgatg tagatggtca tacagaccgc
    20461 cgtctcgaac agtaccgagt tcacgttgaa gtgacccgga atgtagaagt acggcaggtt
    20521 ccagtagcga ccgacgtcga tagtgatcga caagccaccc agtgagtaac cgaacagact
    20581 cgccaacagc gccggacgca ctagcggatg gtattgtcca cggttaaaga cgtataccgc
    20641 ccacgccagc gcccagccgc cacaggcgaa gccggtgccg atcagcaggt caaacgcgat
    20701 ccatacgccc cacgggaaac cgccgttcag gtcagagaca gagcccagac cgaacaccag
    20761 acgcttcaca atcaggagca tacagatgac gattaacggt ccaaaaatca tgaccggttt
    20821 actgatgatt ttgccgccca gcggttgtgg atcatgactc atgatcgtct cctccgtcgt
    20881 gatggtcgtt tttggtgttg cgacgaacca gcacggttaa gcccgccagc acggccagtg
    20941 gtagcatcat gcctttatac agggtgtgtt gaatattttc ggaacgcgca ccggtagaaa
    21001 gatcgtccag cttcggcagg tcgagatttt cataaggcac acccgtcagt accagaacct
    21061 gagtaccgcc gccctctttc tcgccgtaca gatgcggata atatttcggc accgtgtgca
    21121 ggtaagtgtc gccagatttc agcgtctgac gcggatagtg gtattcgctg ccaggcttca
    21181 gcgccaggcg ttttttcgcc tccgccatca gctcttcacg cgtaccaaaa atcaccgcgc
    21241 ccgccgggca cacttctacg cagccaggca gaccgccttt atcaagacgt tccacacctt
    21301 tctggttgca cagttcacac ttatgcagcg caccaaatgg gttgttgtag tcgtacttcg
    21361 gcacgttgta tgggcaggcg accatgcagt aacggcagcc agtgcacaca tctttgtcgt
    21421 aatggacaat gccggttttc ggatcttttt tcagcgcaga gaccggacac acggagacac
    21481 agttcggatc gacgcagtgc atacactgtt tcttaatgta cgcgtagccg ttctcttcct
    21541 ggtctttgtt gacacccgtg ccactggtcc acacctgaat gatgttattg gtatacggcg
    21601 acagtttgtc gttgttcgac caggtctgtt ccccttgcgg gttacgttca gggaagttga
    21661 tatcctgaca cttggtgacg caagcctggc agcctacgca caaggtcgag tcatacaaca
    21721 tccccagcga tcccggaatt ggcgggcggt tttcagcagc cgcatgactg acagacggca
    21781 acgcgcccgt cagcaatgcc ccgcaggagg ctgctttaat aaaattacgt ctgttcacgg
    21841 ttattctccc cgtgagtcag cgttatcttt cttttgctga cgccccagtt cacgcaccgc
    21901 catcacgctg acaccggcaa ccagcccaac cacgccgccg agcaaaccaa tagcgcccgc
    21961 agagacgttg ccgccttctt tagcgttgac gtccggtttt tgtgaacgcg gcgtctggtt
    22021 ttcgacgttg gcaagctgat ggatgccttt atggaagccg ataccttctt cgttacagcc
    22081 atagcatggg tgaccaattg ccaccggcca cacaccgcca acgtcgcaga attgcagcgt
    22141 tgagcagttg ccgtaagttt ctggcccttt acagccgagg tggtacaggc accaaccttc
    22201 gcggtggcct tcatcgccga actctttggc aaaacgacca gcatcgaagt gcggacgacg
    22261 ttcacagtgt tcgtgaatca gacgaccata ggcgaaggtc ggacggtttt tatcatccag
    22321 cttcggcggt ttgccgtaag tgatgatgtg cgcaacggtc gcgaggaagt tgtgcgggtt
    22381 tggcgggcag cctggaatgt taataacggt tttgcctggc agaacttctt gcaggctgac
    22441 cgcgccggta gggttaaccc cagccgcggc aacaccgccc cacgcagagc aggaaccgat
    22501 agcgataatg gctgctgcgc cttccgccgc tttgcggatg tgatccacaa tcggctcacc
    22561 ggcaaccatg caataaatac cattatcttt taatgggatg gaaccatcca ccactaacac
    22621 atactgccct ttgtacttct cgagagcgtt atgtttgttc tcttcgacct gatgaccgaa
    22681 ggcggcggaa agcacttcgt gatactccag agagatagtc tccagcacga ggttttctac
    22741 cgttggatgc gttgcacgaa gcagagattc cgtacaaccg gtacactcct gcgcgccaat
    22801 ccagataact ggcggacgct gcgggttagt aaccgattcg gccatctctg cagcggcttt
    22861 gctacttaac cccatggtgg cggctaatgc tgcacaaagc ttcatgaaat cacgacggtt
    22921 aatgccgtga gaatggatga gggtgttatc tccagtcatt tatagttatt ccgttgcgaa
    22981 gacctggcat atattttgcc tcaatcgcaa aatcaataat gcgatcgatg cgccatttac
    23041 cacacattta ttatggttat cgatatcatg atactgcggc ggggcgctaa aacgaaggga
    23101 agaagcatta gtgtaattaa ttaaagcaag ataatacgta tgtttgatca attttcgagg
    23161 cgataaccgg ccataaaaaa gcgcaacgta gaacaggaat tattctcacg aaagaaatcc
    23221 attaccatct ctttatccag tccatagcgg cgcgtcagaa tacccgcttc ccaggcgcta
    23281 agctggcgat ctccggtctt ttcgtacatc cggtgtgagt agcctaatac aaaaccgcgt
    23341 ttataatcgg cacagtattt tgtcacatta accgcactgt cggcatgcgt tgcttttaat
    23401 cccgccatta agcctttacc aaaatggtta ttcatcgttc acctcattcg ctatatattg
    23461 ccatatatag cgatatttta ggcaaagaac gacttttgcg cacgctattt tttcatgctt
    23521 gtttaagacg taatcccgta agagataaac ctatcaggag agatatttat cgaaccattt
    23581 cagcgtcctt tgccaggcaa gatcggcggc agatttgtca taacggggcg tggaatcatt
    23641 atggaatccg tgattaaccc ccggatagat atacgcctca tagaccttat tattggcttt
    23701 caacgccgcc tcgtaagcag gccagccctc gttgattcgg gtgtccagtt ccgcgtagtg
    23761 gagtagtaaa ggcgcttcaa tcttcgccac atcggcggtg ggtgcctgtc gaccataaaa
    23821 cggcaccgcg caggccagtt ccggatacgc gacagccgcc gcgttcgata cgccaccgcc
    23881 atagcaaaat ccggtaatac ccactttgcc tgtcgcttgc ggatagcgtt gcataaactc
    23941 aattgcggca aagaaatcat tcatcagttt ggttggatca acctgctgtt gcagctcacg
    24001 acctttatca tcatttcccg gataacctcc aacggaactt aagccgtcag gtgccagggc
    24061 gatatacccc gccttcgcca ctcgccgtgc cacatcttcg atatacggat tcagtccacg
    24121 attctcatgc accaccacca cggctggcgt tttgccgctc atctttgcgg gcttcaccag
    24181 ataaccccgt acctcgccgt gaccatttgg tgaaggatac gtgatgtact cagcaacaat
    24241 ttccgggtcg gtaaactcta cctgagtcgc cagcgcataa tttggcttga gcaaatcaaa
    24301 caacgctaat gccgtcatcc cgcctaccgc atacttcgcc gcaaggttga ggaactcacg
    24361 tttcgagatt ttcccgtgag cgtaatagtc atagtaatcc agcaactctt gtaggaaatc
    24421 tttggcggtt aaacgcggca tcattgcact cctcagttgg tgttttttta agcaaagcat
    24481 aagcacgtgt ttttgcccag tttttcgtca ctctgtgagc cagactacgg gatacgcgct
    24541 gatgaatcgc taaactagaa acattgtttc gaaattgaat ggtggaaagg agaggtcatg
    24601 gtctggttag cgaatcccga gcgttacggg cagatgcagt accgttattg cggaaaaagc
    24661 ggtttacgcc tgcccgcgtt atcgctcggt ttatggcaca atttcggtca cgttaacgcg
    24721 ctggaatcac agcgtgcgat cctgcgtaaa gcgtttgatt taggcattac gcactttgat
    24781 ttagccaaca attacgggcc gcctccagga agcgcagaag agaactttgg tcgcctgctg
    24841 cgggaggatt ttgccgctta tcgcgatgaa ctgattatct ctaccaaggc tggatacgat
    24901 atgtggcccg gcccttacgg ctctggcggt tcacgtaaat atctgctcgc cagcctcgac
    24961 caaagcctga agcgtatggg gcttgagtat gtcgatatct tttactctca tcgcgtcgat
    25021 gaaaatacgc cgatggaaga aaccgcctct gcgctggctc atgcggtaca aagcggtaag
    25081 gcgctgtatg tcgggatctc ctcttactcg ccagagcgga cgcaaaaaat ggtcgagtta
    25141 ctgcgcgagt ggaaaattcc gctgttaatt catcaacctt cgtacaattt actgaaccgc
    25201 tgggtggata aaagcggcct gctggatacc ctgcaaaata acggcgtggg ctgcattgcc
    25261 tttactcctc tggctcaggg attgctgacc ggaaaatatc tcaacggcat tccgcaagat
    25321 tcacggatgc atcgtgaagg gaataaagtt cgtggtctga cgccgaaaat gctcaccgaa
    25381 gccaacctca acagcctgcg gttattgaat gaaatggcac agcagcgtgg acaatcaatg
    25441 gcgcaaatgg cgttaagctg gttgctgaaa gatgatcgcg tgacgtcggt attgattggt
    25501 gccagccgcg cggagcaact tgaggagaac gtgcaggcgc tgaataatct gacatttagc
    25561 accgaggagc tggcgcagat tgatcagcat atcgccgatg gcgagctgaa tctgtggcag
    25621 gcgtcttccg ataaatgacc tgttaataac gggcgcgaga accgcgcccg aatcagatca
    25681 gtgattatga cgagtcagtc ggtcaagata gcccatcaca aatgcagaga gcacaaacgt
    25741 cagatggata atgacgtacc acatcagttt gttatcaggg acatttttcg catccataaa
    25801 gacgcgcagt aagtgaatgg aagaaattgc cacaatcgac gctgctactt tgtttttcag
    25861 cgacgttgcg tccattttcc ccagccagtt cagcttctct ttgttctcgg agatatccag
    25921 ttgtgagacg aaattctcat aaccggaaaa catcaccatc accagcaaac cgccaaccag
    25981 cgtcatatcc accagcgaca gcaacacgag gatcaaatct gattccgcca tcgagaagat
    26041 attcggcagt acgtgaataa tctcctggaa gaacttcagc gccagggcaa ctaacgccag
    26101 cgaaaggcca aagtacacgg gggcaagcag ccagcgagaa gcatacattg cattttcaag
    26161 aaaacgttcc atacattcct gtcgtgtagt taaaacgggc aacagtatat cgcaattgag
    26221 taaacagcgc gcaacagcaa atcaacatat ccgttaaacc tttgtcgcat aaatccgagg
    26281 tttagggatc ttcctggttc ccttatcaac gcatcgagag cgtctaccct taaaggacgt
    26341 tgaacctgaa gggagaaaaa cgatgtctca tttaaaagac ccgaccacgc agtattacac
    26401 tggtgaatat cccaaacaga aacaaccgac gccaggcatc caggcgaaaa tgacaccggt
    26461 gccggattgc ggcgagaaaa cctatgttgg tagcggtcgc ctgaaagatc gtaaagcact
    26521 ggtgacaggg ggcgattccg gaatcggtcg cgctgccgcc attgcttacg cgcgtgaagg
    26581 ggctgacgtg gcgatcagtt atcttcccgt agaagaacaa gacgctcagg atgtgaaaaa
    26641 gatcattgaa gaatgcggac gcaaagccgt cctgctgcca ggcgatttaa gcgatgagaa
    26701 attcgcccgt tcgctggttc acgaagcgca taaggcgtta ggcgggctgg atattatggc
    26761 gctggtcgcc gggaaacagg ttgccattcc tgatattgca gacctcacca gcgaacagtt
    26821 tcaaaagacc tttgccatta acgttttcgc gctgttctgg ctaacccagg aagcgatccc
    26881 cctgctaccg aaaggtgcca gtatcatcac cacttcgtca atccaggcat accagccaag
    26941 cccgcattta ctggactatg cggctacgaa ggcggcgatt ctgaactaca gccgtggcct
    27001 ggcaacacag gtcgcagaga aaggtattcg ggtgaatatt gttgcgccag gcccgatctg
    27061 gacagcactg caaatttccg gcgggcaaac gcaggataag atcccgcagt ttggtcagca
    27121 aacgccgatg aaacgtgcgg ggcaaccggc ggaactggcc cctgtatatg tttatctggc
    27181 aagtcaggag tcgagctacg tcaccgcaga agtgcacggc gtgtgcggcg gcgagcattt
    27241 aggttaaaaa aatgcccggt tgtgaaaagc aaccgggcat catttttatt tcgctttggc
    27301 ggtttcttcg ccgaccagac ctatcttcag ataacccgcc tgatgcagcg tatccattac
    27361 cttcatcaac gtctcgtaat cgacggtttt gtccgctcgg aagaagatgg tggtgtcttt
    27421 cttgccttcg gttaacgcat tcaacgccgt aatcattgtt tcatcggtga ccggatcgtt
    27481 accgataaac atcgagttgt ctgccttcac cgacagataa accggttttt ccgggcgcgg
    27541 ctgcggcgtg ctggtagaag caggcaagtt caccttcaca tctaccgtcg ctaacggtgc
    27601 cgccaccata aagataatca gcagaaccaa catcacgtcg ataaacggcg tcacgttgat
    27661 atcatgcatt tcgccgttat cgtcgaggtt ttcgttaaga tgcattgcca tcggacatta
    27721 tcctgcgcgt aatttttgtg cgacacgaac cggatgcgca gcggcgctgg cttccagatc
    27781 caggtcacgg ctttgcagca acaatacctg cgctgcgaca tcacccagca tcgctttaaa
    27841 gccgccaatc tgacgtgcaa atacgttata gataacgacc gccggaatcg ctgccacgag
    27901 gccgattgcc gttgctaaca gagcttctgc aatacccggc gcaacgactg ccaggttagt
    27961 ggtctgcgtt tgcgcgatac caataaagct gttcatgatg ccccagaccg taccaaacag
    28021 accaacgaac ggcgaaatcg cgccgatggt tgccagatag ccgttaccgc ggcccatttg
    28081 acgacctact gcggcgaccc gacgctccag acggaagctg gtacgttctt taataccttc
    28141 gttatcgtcg ctgccttctg acagttccag ctcattctgc gcttcattga gcaaatgcag
    28201 gcttaagctt ttgctaccaa aatcagcggc aatatcgttg gcctggttta aggaacgcgc
    28261 ttcagccagc agttgctgct cgcgcttaag gcgacgcttc tgattgaaga actctacgct
    28321 cttactgaag aagattgccc aggtgactac ggaggccaaa ataagcccaa tcatcacgca
    28381 cttaacgaca atatcggcgt gctgatacat accccagacg gaaaggtccg tctgcattaa
    28441 attattaccc actgtgtatc tccaggacgc aagtcacaaa atctgcgcat aataatatca
    28501 aaacgacgtc gaattgatag tcgttctcat tactatttgc atactgccgt acctttgctt
    28561 tcttttcctt gcgtttacgc agtaaaaaag tcaccagcac gccatttgcg aaaattttct
    28621 gctttatgcc aattcttcag gatgcgcccg cgaatattca tgctagttta gacatccaga
    28681 cgtataaaaa caggaatccc gagatggcgg acaaaaagct tgatactcaa ctggtgaatg
    28741 caggacgcag caaaaaatac actctcggcg cggtaaatag cgtgattcag cgcgcttctt
    28801 cgctggtctt tgacagtgtg gaagccaaaa aacacgcaac gcgcaatcgc gccaatggcg
    28861 agttgttcta tggacggcgc ggaacgttaa cccatttctc cttacaagaa gcgatgtgcg
    28921 aactggaagg tggcgcaggc tgcgtgctat ttccctgcgg ggcggcggcg gttgctaatt
    28981 ccattcttgc ttttgtcgaa cagggcgatc atgtgttgat gaccaacacc gcctatgaac
    29041 cgagtcagga tttctgtagc aaaatcctca gcaaactggg cgtaacgaca tcgtggtttg
    29101 atccgctgat tggcgcagat atcgttaaac atctgcagcc aaacactaaa atcgtatttc
    29161 tggaatcgcc gggttccatc accatggaag tccacgacgt tccggcgatt gttgccgcag
    29221 tgcgcagtgt ggtgccggat gccatcatta tggtcgataa cacctgggcg gccggtgtgc
    29281 tgtttaaggc gctggatttt ggcattgatg tttccattca ggccgctacc aaatatctgg
    29341 tggggcattc agatgcgatg attggtactg ccgtatgcaa tgcccgttgc tgggagcagc
    29401 tgcgggagaa tgcctatctg atgggccaga tggtcgatgc cgataccgcc tatataacca
    29461 gccgtggcct gcgcactttg ggtgttcgcc tgcgtcaaca tcatgaaagc agtctgaaag
    29521 tggctgaatg gctggcagaa catccgcaag tagcgcgagt taaccaccct gctctgcctg
    29581 gcagtaaagg ccacgaattc tggaaacgag actttacagg tagcagcggg ctattttcct
    29641 ttgtgcttaa gaaaaagctc aatgatgaag agctggcgaa ctatctggat aacttcagtt
    29701 tattcagcat ggcctactcg tggggcggtt atgagtcgtt gatcctggca aatcaaccag
    29761 aacatatcgc cgccattcgc ccgcaaggcg agatcgattt tagcgggacc ttgattcgcc
    29821 tgcatattgg tctggaagat gtcaacgatc tgattgccga tctggatgcc ggttttgcgc
    29881 gaattgtata acattgccac ttttggacaa ttttgcagac attttattgt gaaaagtctt
    29941 aaattgttgc gtccgggatc aaggcgtccc ggacgaatca ggagtacaat aggcagataa
    30001 aggcttaaac gctgttccac aggaaagtcc atggctgtta ttcaagatat catcgctgcg
    30061 ctctggcaac acgactttgc cgcgctggcg gatcctcata ttgttagcgt tgtttacttt
    30121 gtcatgtttg ccacgctgtt tttagaaaac ggcctgctgc ccgcctcatt tttgccaggc
    30181 gacagcttgt tgatattggc tggcgcattg attgcccagg gggttatgga ttttctgcct
    30241 acgattgcga ttctgaccgc cgcagcaagt ctgggctgct ggttaagtta tattcagggg
    30301 cgctggttag ggaataccaa aacggtgaaa ggctggctgg cacagcttcc tgctaaatat
    30361 caccagcgcg ccacctgcat gtttgaccgc cacggtctgc tggcgctgct ggctggacgt
    30421 tttcttgcat ttgtccgtac gctgctgcca accatggcag gaatttccgg tttgccaaac
    30481 cgccgtttcc agtttttcaa ctggttaagc ggattgctgt gggtcagcgt ggtaaccagt
    30541 ttcggctatg ccttaagtat gattccgttc gttaaacgcc atgaagatca ggtaatgacg
    30601 atcctgatga tcctgccaat tgccttgtta accgctggct tgttaggcac gctgtttgtg
    30661 gtgattaaaa aaaaatactg taacgcctga cgattttccc cgttcccggt tgctgtaccg
    30721 ggaacgtatt taattcccct gcatcgcccg cattcttgcc gcatcttccc ccggcgtcac
    30781 accgaagtaa cgtttaaact cacggctaaa ttgcgatgcg ctttcatagc cgacgcgcat
    30841 cgccgccgcg ctggctttca tgccgtcgtg gatgatcatc atccgcgcct tatgcagacg
    30901 gtaattcttc aaatactgca acggcgaggt gctagtgaca gacttaaaat tatggtggaa
    30961 cgccgatacg ctcatgttgg cttctgccgc cagttgctcg acgctcaggt tttcggtgta
    31021 tttattctca atccgtttca gcacgcggct aatcaggctg aagtgagtct ggcgactgac
    31081 cagcgccagt aacgcaccgc cgcaaggtcc ggtcagcacg tagtacagaa tttcgcggat
    31141 gatctgtttg ccgagaatac gcgcatccag cggtcgctcc atcacgtcga gtaaccgctc
    31201 cgccgcgcat aaaatctctt ctgataacgt ggcagagtta atcccgctgg ctgccatcga
    31261 cggctggaaa tgctcatctt cgccaatgtc catcaacagt tcctgtaact gcaaaatatc
    31321 gacattgaga cgcaaccctg ccagcggcac ctctgacgtg gcataggttt cgcactcaaa
    31381 cggcaacggc accgtcagca gcaggtattc attggcatca taacgaaaca cgcgttcatt
    31441 gatataaccg attttatgcc cggaaaagag aattatgatg ccaggctcgt acattaccgg
    31501 tgtacgtgcg aaaggcgtct cgccatacaa caaacgcaca tcgggcaaca gctctgacaa
    31561 accattttct ttatttttca gtttattaac tttatccgcc agcaagcggc aaatctcttc
    31621 acgtttcata tcgcgtaatt tcttaggaat aatgcggcaa tttgattgtg cgcaattttg
    31681 tagcatttct ccagcactct ggagaaatag gcaagacatt ggcagaaatg agcattgaga
    31741 ggtacagcgc tggcgaccac aatgaaaaac atcaggcaga tcgttctctg ccctcatatt
    31801 gacccagcaa agggagcaag taatgaacaa ctttaatctg cacaccccaa cccgcattct
    31861 gtttggtaaa ggcgcaatcg ctggtttacg cgaacaaatt cctcacgatg ctcgcgtatt
    31921 gattacctac ggcggcggca gcgtgaaaaa aaccggcgtt ctcgatcaag ttctggatgc
    31981 cctgaaaggc atggacgtgc tggaatttgg cggtattgag ccaaacccgg cttatgaaac
    32041 gctgatgaac gccgtgaaac tggttcgcga acaaaaagtg actttcctgc tggcggttgg
    32101 cggcggttct gtactggacg gcaccaaatt tatcgccgca gcggctaact atccggaaaa
    32161 tatcgatccg tggcacattc tacaaacggg cggtaaagag attaaaagcg ccatcccgat
    32221 gggctgtgtg ctgacgctgc cagcaaccgg ttcagaatcc aacgcaggcg cggtgatctc
    32281 ccgtaaaacc acaggcgaca agcaggcatt ccattctgcc catgttcaac ccgtatttgc
    32341 cgtgctcgat ccggtttata cctacaccct gccgccgcgt caggtggcta acggtgtagt
    32401 ggacgctttt gtacacaccg tggaacagta tgttactaaa ccggttgatg ccaaaattca
    32461 ggaccgtttc gcagaaggca ttttgttgac gctgatcgaa gatggtccga aagccctgaa
    32521 agagccagaa aactacgatg tgcgcgccaa cgttatgtgg gcggcgactc aggcgctgaa
    32581 cggtttgatc ggcgctggcg taccgcagga ctgggcaacg catatgctag gccacgaact
    32641 gactgcgatg cacggtctgg atcacgcgca aacactggct atcgtcctgc ctgcactgtg
    32701 gaatgaaaaa cgcgatacta agcgcgctaa gctgctgcaa tatgctgaac gcgtctggaa
    32761 catcacagaa ggttccgacg atgagcgtat tgacgccgcg attgccgcaa cccgcaattt
    32821 ctttgagcaa ttaggcgtgc ctacccacct ctccgactac ggtctggacg gcagctccat
    32881 cccggctttg ctgaaaaaac tggaagagca cggcatgacc caactgggcg aaaatcatga
    32941 cattacgctg gatgtcagcc gccgtatata cgaagccgcc cgctaagctt tttacgcctc
    33001 aaactttcgt tttcgggcat ttcgtccaga cttaagttca caacacctca ccggagcctg
    33061 ctccggtgag ttcatataaa ggaggaacgt atggctaatc caaccgttat taagctacag
    33121 gatggcaatg tcatgcccca gttgggactg ggcgtctggc aagcaagtaa tgaggaagta
    33181 atcaccgcca ttcaaaaagc gttagaagtg ggttatcgct cgattgatac cgccgcggcc
    33241 tataagaacg aagaaggtgt cggcaaagcc ctgaaaaatg cctcagtcaa cagagaagaa
    33301 ctgttcatca ccactaagct gtggaacgac gaccacaagc gcccccgcga agccctgctc
    33361 gacagcctga aaaaactcca gcttgattat atcgacctct acttaatgca ctggcccgtt
    33421 cccgctatcg accattatgt cgaagcatgg aaaggcatga tcgaattgca aaaagaggga
    33481 ttaatcaaaa gcatcggcgt gtgcaacttc cagatccatc acctgcaacg cctgattgat
    33541 gaaactggcg tgacgcctgt tattaatcag atcgaacttc acccgctgat gcaacaacgc
    33601 cagctacatg cctggaacgc gacacacaaa atccagaccg aatcctggag cccattagcg
    33661 caaggaggga aaggcgtttt cgatcagaaa gtcattcgcg atctggcaga taaatacggc
    33721 aaaaccccgg cgcagattgt tatccgctgg catctggata gcggcctggt ggtgatcccg
    33781 aaatcggtca caccttcacg tattgccgaa aactttgatg tctgggattt ccgtctcgac
    33841 aaagacgaac tcggcgaaat tgcaaaactc gatcagggca agcgtctcgg tcccgatcct
    33901 gaccagtttg gcggctaacg agcaaattct cccggtggcg gtaatgttcc gctaccggac
    33961 ttttcagaaa tcatttattc ccctcgcgtc ctgcccattg ttactcttcc ttgttcagga
    34021 atgccaaata taaggacatc atcatgcaga gccggaagct cttaaaggaa caactcatct
    34081 acacccggga taaacgcaac ggagaggtga aaaacagatg aaaataatac ttctgttttt
    34141 agcagccctg gcaagtttta ccgtacacgc gcagcctcct tcacagaccg tagaacaaac
    34201 agtccggcag atttatcaga actataaatc agatgccagt acgccttatt tcggtgaaac
    34261 cggagagcgg gcgataactt cagcgcgtat tcaacaggcg cttaccctga acgacaatct
    34321 tacactaccg ggcaatattg gctggctgga ttatgatccg gtttgtgatt gtcaggattt
    34381 tggcgatctg gtgttagaaa gcgtagcgat aacccaacct gacgccgatc atgccgatgc
    34441 cgttgtgcgc tttcgtatct ttaaagacga taaagaaaag accatgcaga cactgaaaat
    34501 ggtggcggaa aatggtcgtt gggtcattga cgatattgtc agcaatcatg gcagcgtctt
    34561 acaagcagtt aatagcgaga atgaaaaaac gctggccgct atagcttcgt tgcaaaaaga
    34621 acagccggaa gcctttgttg ccgaactctt tgaacatatt gctgattata gctggccgtg
    34681 gacgtgggtg gtttccgact cttaccgcca ggcggtcaat gccttctata aaaccacctt
    34741 catgactgcc aacaatcccg atgaagatat gcaaatagaa cggcaattta tttacgacaa
    34801 tccgatctgt tttggcgaag agtcgttatt ttcccgcgtt gatgaaattc gagtcctgga
    34861 gaaaaccacc gattccgccc gcattcatgt tcgttttacg ctgaccaatg gcaacaacga
    34921 agagcaagaa ctgattttac agcggcgcga aggtaagtgg gaaatcgctg attttatccg
    34981 cccgaacagc ggcagcctac ttaagcagat tgaggcaaaa accgccgcca gattaaaaca
    35041 atgagctgaa ttaaataaca attagccgga acaataaata aaagggaaca ctatatgaaa
    35101 acgattttca ccgtgggagc tgttgttctg gcaacctgct tgctcagtgg ctgcgtcaat
    35161 gagcaaaagg tcaatcagct ggcgagcaat gtgcaaacat taaatgccaa aatcgcccgg
    35221 cttgagcagg atatgaaagc attacgccca caaatctatg ctgccaaatc cgacgctaac
    35281 agagccaata cgcgtcttga tgcacaggac tattttgatt gcctgcgctg cttgcgtatg
    35341 tacgcagaat gataaaaaaa ttcccggcag cattctgtta ccggggatat tttttaacgt
    35401 ccaaccgccg ctttagggcg tttcttcgca ccagcattca ccggacgaga ttgcgtagac
    35461 gacgcttttt ttgccgtagc aggcgtctga cgctgggtcg ccatcggcgt atgtttagtc
    35521 aacgccggac gggtattgcg gttctggcga cgagcctcac gcatctcttc aatggttggc
    35581 gcaggcacta agcaatcgcg acggctgcca atcagatgct ttttgcccat cgcttccagc
    35641 gcctgacgga ttaacggcca gtttgccgga tcgtggtaac gcaacaacgc tttatgcaaa
    35701 cgacgctgct tgtcgccctt cggtacgaag acgtcttcgc tcttataacc aatcttcgcc
    35761 agcgggtttt tgccggtgta atacatggtg gttgagttcg ccagcggcga cggatagaag
    35821 ttctgcacct ggtcgaggcg gaagcggtgc tttttcagcc acagcgccag attcaccata
    35881 tcttcatcac gcgtaccggg gtgcgcggag atgaaatacg ggatcagata ctgctcttta
    35941 cctgcctgtt tcgagtaggt atcgaacaac tctttaaagc ggtcatagct gcccatgccc
    36001 ggcttcatca tcttcgacaa cggcccttct tcggtatgtt ccggggcaat cttcagataa
    36061 ccgccgacgt gatgggtcgc cagctctttg atatagcgcg gatcttccac ggcgatgtca
    36121 taacgtacgc cggaagcgat caggatcttt ttaatgcctt tcagatcacg cgcacggcga
    36181 tagaggttga tcgtcggttc gtggttagtg tccatatgcg gacaaatatc cgggtaaaca
    36241 cacgacaagc ggcggcaggt ctgttcagcg cgtggcgatt tgcagcgcaa catatacatg
    36301 ttggcagttg gcccaccgag atcggaaatt acgcccgtaa aacctggaac ggtgtcgcgg
    36361 atcgcttcga tctcattgat gatcgactct tccgaacggc tctgaataat gcgcccttca
    36421 tgctcggtaa tagaacagaa agaacagcca ccaaagcagc cacgcataat gttgaccgag
    36481 aaacggatca tttcgtaagc cggaatacga gcattgccat aggccggatg cggcacgcgc
    36541 ttgtacggca gcgcaaaaac gctgtccatc tcttcggtag agagcggaat agcaggcggg
    36601 ttgatccaca catagcggtc gccatgtttt tgcatcaatg cgcgggcgca gcctgggtta
    36661 gtttcgtggt gcagaatacg cgaagcatgg gcgtacagca ctttatcgcc cttcactttc
    36721 tcgaaagaag gcagcaatac gtaggttttt tcccacggtt tcgggcgcgg cggctgcacg
    36781 gttacggctt tcgcttcctg ctttttcggc gcaacaggtt tgttatccgc gcacggcaga
    36841 tcttcgccat acggatgcgg gatcgggtcg attttccctg gggtatcgag acgggtggaa
    36901 tccacgccgc tccagcctgg caacgcctct tttacgataa tcgcagtatt acgcacatcg
    36961 cggatttcac taatcggctc gcccatcgcc agacggtgcg ccacctccac caacggacgc
    37021 tcaccgttac cgaacatcag catgtcggct ttggaatcca ccagcacgga acggcgcacg
    37081 gtatccgacc agtaatcata gtgcgcggta cggcgcagac tagcctcaat accgccgagg
    37141 atcaccggca catctttcca cgcctcttta caacgctggg tataaaccag tgtggcgcga
    37201 tccggtcgct tacccgcgac gttatccggc gtgtaggcat cgtcatgacg taaacggcga
    37261 tcggcggtat agcggttgat catcgagtcc atgttgccag cagtaacacc gaaaaataga
    37321 ttcggtttac ccagacgcat aaagtcgtct ttgctgctcc agtccggctg ggcgatgatc
    37381 ccgacgcgaa agccctgcgc ttccagcata cgaccgcaaa tcgccatccc gaagcttggg
    37441 tgatcgacat acgcgtcgcc agtaaccaaa atgatgtcgc agctatccca gccaagttga
    37501 tccatctctt cacgagacat cggcaaaaac ggtgccggtc caaaacaggc ggcccagtac
    37561 tgcggccagg agaacaggtc gcgatccggt tggatcaggg agatagagct cattttgctt
    37621 ccagaaatga taaaaaaata atcaaaggcc ggggattata agccggaacg aaagagaaat
    37681 cgaaaggtat tccatactcg ccctcctcgg gcgagtatga agattacggt accgggttga
    37741 ctaacagttg cccaatcgag ccacggtccg ccatttccag cgtctgactg ttgaagtaga
    37801 acgggaagtg cgcccaggaa ggctgaccga aataaacaag cagctccact tgtccgtcaa
    37861 cccaaacggt atctttccag cctctgtctt ccgggaacgg catcgcgcca ttcacgttac
    37921 ggatctggaa catcacgcct tcaatatgga acgcttgcgg ctcgtccgcg cgtaccgtcc
    37981 agcgttccca cgttccttgc tgcgcggtga catcaatacg gttgacgtcc cacagctgcc
    38041 cattaatacc cgggtcatca cccagactga tatcgcgact gcgaatcggc gaaccggcca
    38101 tgatttcagt tggcagcaag cgcatcggaa gactgtctgt gaccagcggc agaaggccgg
    38161 ttgggcgcag cgttagcacc agggtagaaa ccagaatgct ggatggctca aagaagccac
    38221 gaatacgatc aacaatgctc gccgcttcgc cacaggtgat cgacacttca tcaccgttgc
    38281 tcatatccac cagaatctca cgacgctcgc ccggtgccag cgaaagttgc ttcaccgata
    38341 caggagcggg caggaatccc tgatcgccag aaatcacatg taacgggcga ccatcgctca
    38401 tctgtagttg atagcgacga gagttcgacg cgttcaacag acgcaagcgc acccagccac
    38461 gcgagacttc aacgtacggg ctttgtacac cgttaaccag cagcgtatca ccaacaaagc
    38521 cgccgcttcc cggttcgttg tattctggcg taccaaagtt gtccagccgt ttatcctgga
    38581 taatgaccgg aaaatcatcc acaccataat ggttggggat aggcagcgac ttgctgactt
    38641 catcttccac cagccacatt ccggcaaggc cgttatagac ctgctgagcc gtgcggttgg
    38701 gagtattggc gtgataccac agagtagctg cgttctggcg aataggcagt acgggtgccc
    38761 agtcagcgtt tggcgacatc atccgtgccg gaccgcccat cagcgggcca ggtacctgta
    38821 gcccggccac cgtcattgag acattttctg tcaggcggtt gctgtaaata agcttaacat
    38881 cgtcgccctt ccagacgcgg atagtcggcc ccaggtaacg accattgatt ccccaaaccg
    38941 acgcgcgtgt ccctggcgta aatgaccagt gcgcacgttg tacagtcata aacagcggtt
    39001 gcccacggcg agattcaagc agcggcggaa cgggtagcgg ttgctgttgc ccggctgcgc
    39061 tggccttcag gggaacagcg cctgcacaaa gtgcaatccc cgatgcctga atgaactgac
    39121 gccgactgag tgacatattt gctccatgta aaactggcta aagtaatacc gtgcaggaaa
    39181 gtgttccccg caaaaataca gattgccgac ttaaactttt ccggcggctt cgcgctctgc
    39241 gacttcttta tcgagatcag cgattttttg ttccattatc gaacgacaat gggcagccaa
    39301 ctcgcgaacc tgatctttgc catactgact gacgtcaatt ggcggcagca tttcgacaat
    39361 caccagaccg ttgtgtagcc gattaagatt aatcttattc gaagttgtag agacgcacac
    39421 gggaataatc gggacgcccg ccgcaattgc cgcgtgaaat gccccggtct tgaacggtag
    39481 caggccgcga ccacggctgc gggttccttc cgggaacatc cagatggaaa tacggcgttt
    39541 tttgaagtga ttcacgactt ccgcaatagt gccgtgagct ttagtgcgat tgtttctgtc
    39601 gatcaatagg ttgccggtta accagtacaa ctgcccgaag aaggggatcc acagcaagct
    39661 ctttttacct accgtcaccg tcggcggttg cacaatgttc gatgctgtca ccatgtcata
    39721 gttgttctgg tggttagcga tatagatagc attgccgtag ctttcagcat ctgcaggttt
    39781 gcggcactca actttcaggc cgaacagcgg cgcaagacga ccaaacatat gcccaaaggt
    39841 ggccacatgt ttcgggttac gcgggctgaa aaggcagtaa atggagccga atacacagac
    39901 taagatgctg taaatcacgg taataataag acgaaagata tatagcataa caccctctga
    39961 aggtctgaga gactggcatt gtacgtcacc tgaaatcagg ttagggcatt gttaaaagcc
    40021 gctctctgga aaagcgtatt gttaatcgca acgcatgaat aaacaacggt ttcaagggaa
    40081 tttttatcgt ttccgggggt tgaatacgct gccggatgac gacttaacgt ttcatccggc
    40141 gttccttgca agcgggagga aacagcgccc tccccggcat attactcttc gctatcaccg
    40201 ctgctggcac ggcgaggaga gtcgatctca acacgatcga tacgctgcaa accgcgcatc
    40261 aacgtaccgc ggcgtccacg ttcgccagtg actttctgta gctcttccgg acgcagttta
    40321 attttgcgtt tcccaacatg aatggtcagc gtgcttagcg gcggcagaac gtacagttgc
    40381 gccagaccat cttctccacg cgcggcttct gccgatggaa tgttgataat cttgttgcct
    40441 ttgcccttcg acagctgcgg cagatcgctt accgggaaca tcaacatacg gcctgcctga
    40501 gtgattgcca gcagcatatc agaagcatct tcaatcacca ccggcggcat aacatgggca
    40561 ttttccggta aggtgatcaa agctttacct gcacggttac gcgccaccag atcgttaaaa
    40621 gtgcagacga aaccgtaacc cgcatcggaa gccatcagca gtttctgatc gtcgctttcc
    40681 atcagcatat ggtcaacggt cgctccaggc ggcaacgtta atttgccggt gagcggctcg
    40741 ccctgaccac gcgccgacgg cagcgtaatc gggtcgatgg catagctacg accggtggaa
    40801 tcaacaaaca ctaccggttg gttgctctta cctttcaccg ccgctttgaa gctatcgccc
    40861 gctttataat tcaggcccgg cgcgtcgata tcatggcctt tagcgctgcg tacccagccc
    40921 atctgcgaca gcacaatggt gacaggttca gacggcagca tgtcgtgctc gctcatcgct
    40981 ttcgcttctt cgcgttcctg caacggcgaa cgacgttcgt caccgtaggc ttgcgcgtct
    41041 gcctgcagtt ctttcttcag caggttattc attttacgct cggaagccaa aatgccctgc
    41101 aactggtcgc gctctttttc cagctcactc tgctcaccgc gaatcttcat ctcttccagt
    41161 ttggcaagat gacgcagttt cagttcgagg atcgcttccg cctgggtttc cgtaaggcca
    41221 aaccgcgaca tcagcgccgg tttcggttca tcttcattac gaatgatctc aatcacttcg
    41281 tcgatattga gaaacgccac cagcaaacct tcgaggatat gcaggcgctt gaggactttc
    41341 tccagacgat agttcagtcg gcggcgcacg gtatcgcggc ggaacaccag ccattcggag
    41401 agaatttcca gcaggttttt caccgccgga cgaccatcca gaccgatcat attgaggtta
    41461 atacgatagc tcttttccag atcggtggtt gcgaagaggt ggttcatcac ctgatccata
    41521 tccacgcggt tggaacgcgg cacaatcacc agacgagtcg ggttctcgtg gtcagattca
    41581 tcgcgcagat cgtcaaccat cggcagcttt ttgttgcgca tttgcgcagc aatttgctcc
    41641 agtacgcgcg cacctgaaac ctgatgcggc aatgcgctga taaccaccgc gccatcttct
    41701 ttcttccaca ccgcgcgcat acgcactgaa ccacgtccgt tctcgtagat tttacggatc
    41761 tcggcgcgcg aagtgataat ttccgcttca gtcggataat ccggcccctg cacgatatcc
    41821 agcagctggt cgagcgtggt tttcggctgg tcgattaatg cgattgccgc ctgagccact
    41881 tcacgcaggt tatgcggtgg aatatcggtc gccatgccga cggcaatacc ggtggtgccg
    41941 ttaagcaaaa tgtttggcag acgggcaggt agcattttcg gctcctgcaa agtgccgtcg
    42001 aagtttggta cccagtcagc cgtcccctgc cccagctcgc tcaatagcag ctcggaatat
    42061 ttcgacaacc gggattcggt gtaacgcatt gccgcgaacg atttcggatc gtccggcgcg
    42121 ccccagttcc cctgaccatc aaccagcgga taacggtaag agaacggctg cgccatcagg
    42181 accatcgctt cataacaggc gctatcgccg tgcggatggt atttacccag tacgtcaccg
    42241 acggtacggg ccgatttttt aaatttggcg ctggcattca ggcccagctc agacatcgca
    42301 tacacaatgc ggcgctgaac aggtttcaga ccatcaccaa taaacggcaa cgcgcggtcc
    42361 atgatcacgt acatggagta gtttaaatag gcgttttccg taaattcatg tagcgcaagg
    42421 cgctctgcca tatcgctcat taattctgat tcctcaactt attcgccagc cttcaaaggg
    42481 gcaatattgc cggagatact acattatctg ccgcgtcgag tcacaggaga tcagcgtgaa
    42541 tagcgccgcc ggacgtgctc cgacggcgat aaaggaatca atgtgccttg atatacaact
    42601 ctttgctgta gacataatcc tggggattat gcagcggaaa accgccaacg tagggtttaa
    42661 gcagtttgat taacggctga tagtagatag gaatcagcgg tgcctgttgg ttgatgatca
    42721 cttctgcctg ctgatacaac gcattacgct ttgtcgcatc agtgatctgc gtggcctggt
    42781 ttagtaaggc gtcatactgc gcatttttcc agtgaccgac gttttcttca ctatcgcttt
    42841 tgagcgtgtt caggaagctg gaagcatcat tgtacgtcgc atcccacgac tgccgcgaca
    42901 acatgaaatc accggctcgt cgggcatcaa gataagtttt ccactccatt gtgcgcagcg
    42961 tcacctgtgc acccagccat tttttccatt cggaagacaa cgctatcgcg gttttttcat
    43021 gcagatcgta cttgttgtag aacagctcaa agcgtagcgg atgagaggcg tcgtatcccg
    43081 cctgtttcag caaggctttt gccatcgcga cacgctcact cattggcttt tgcagttcat
    43141 cgaacgtcgt cgcgctaaag ccttttacct ctggcggcgt cagcgtggtt gcgggcgttc
    43201 tcaaccccag taccttttgc gcaataagct gtcgatcaac cgtaagatat agcgcccgac
    43261 gcacccgcac atcgttaaat ggcggtttct caaggttaaa gttgtaatat tcgctgttca
    43321 gccgcgggat aattcgtagc tcgccaggca gtgatttttc aatggcggga atttgctgcg
    43381 ccggaaccca ggtgagatcg acctctcccg cgcgatagcg gttatagccg gtgaccgaat
    43441 tacccagcgc cagatactca acctgttgca atactgtatg ttgcgcatcg cggtactttg
    43501 gatttttgcg tgcagtaatc ttttcgttaa ctacccactg atcgagcaca aaggcaccgt
    43561 tgtaaaccat gttctctggc ttactccagc tatcgccatg tttagcgatg acatgatgag
    43621 gaaccgggaa cagcgtcggc caggcgagca tcgtcgtgaa ccaaggaacc ggctgctcaa
    43681 gcgtaacttc aagagtacga tcatccatcg ctttgacacc cagcgatgta acatctgctt
    43741 tacccgcaac aatagctgcg gcattgttaa tgtgcgcctg tgccagatag ccagcaaaag
    43801 ggcttgccgt tttcgggtca accgcgcgct gccagccgag gacaaaatcc tctgccgtca
    43861 gaggctgacc gtctgaccac tgcaaaccgc tacgcagatg gaaaatatag cgcttgccgc
    43921 cgtccagtat ctcccagcgt tcagcctgag cgggctgcac ctgaccttca ccgtccatcc
    43981 ataccagacc ttcaaacaga tccagcacaa tctgcgcggc agtattctcc tctacctttt
    44041 gcgggtcgag cgtgcctggg tcgctatgat tgttgtaacg aaaaacttgt tgcggggcga
    44101 gcggtgtgtt ggcgggaacg tcagcagcgt agagaggagc cgcacttacc aggctgacca
    44161 gccataataa atttcgcgta tacatggtat tccttaccgg agaaattata agcccaacaa
    44221 caatacgcct gtggcattgt tcgctcaaac ttatcgcgag tgatttggct cacactccgg
    44281 taaagattct tcatcacgcc aacggcacat aaacatctgt ttccagtcgc tgctctgtca
    44341 tcccttcggc aagattggtg tagtgaaaca gaatcggtgc tttacgcatt ttctcgccgc
    44401 ttgcaggtag ccagtggcga atgatgcccc atatcgtgtg tgaaatatcg tccagctcgc
    44461 caacatggcg ggccacagca taacgtccac cggtaagttc accattactc acaccataac
    44521 gattatcggg gatcggttcg ctaacgctgc cgcagatatc aaagcgaaac gcttccggtg
    44581 cggtggtggc agggtcatcc caggcgacgc caaaagtctg gctttggtta acaggagaaa
    44641 gccccgtctc tttacgccac atgataaatt tcgccacgct gtagttgagc aactcagggc
    44701 tacagcggtg cggcaacatc gccacaggga ttgatgggaa atcgataata tttacatcca
    44761 gtgtcaggtt tgtcattttg cctccctgcg aaattattcc cgggaatgtg tcccgggagc
    44821 ggtaacaatt acggatttac tttgcggatc tgtttgacgt caatttcaac agaattccag
    44881 tctttatcga cttcaccctg aatctcaacc gtatctttcg gcgtcaccgt cacgccgttc
    44941 cagcgtttgt ggtcgatatc aacattgata gtaccgctgg catctttgaa cacgtagaga
    45001 tcgtcagaga tgcgttcaac gatattgccg cgcagggtta cccaggtgtc gtcacgcagg
    45061 gattttgcgc tttctacggt cgttacgctg ccgttcggcc cctggaatcc tccggcctga
    45121 ctttgcgttg ccgatgggcc agaaaaaccg ccctgctctg ctgccatcac cggtgcgctg
    45181 cacagggcca ttactgcgat tactgctgcg aattttttca tgtttattac tccctttaat
    45241 gtctgtttcc gagcatttaa caagatagtc cttaacaact tcttaaggga aaaaaataaa
    45301 atttaatgct gtacagagcg cgttacaaca cggtttactg gcagcaaata cggttatcgc
    45361 agggatgaaa aaatgcgaat tttactgata gaagatgaca tgctgattgg cgacggcatc
    45421 aaaacgggcc ttagtaaaat gggttttagc gtcgactggt ttacacaagg tcgtcaggga
    45481 aaagaggcgc tatatagtgc gccttatgat gcggtgatcc tggatttaac cttaccaggc
    45541 atggatggtc gcgatatttt gcgcgaatgg cgagaaaaag gtcagcgtga gccggtactg
    45601 atcctgaccg cgcgcgatgc gttagcggaa cgtgtagagg ggctgcgtct gggagctgac
    45661 gattatctgt gtaaaccttt tgcgttgata gaagtcgccg ccaggctgga agctctgatg
    45721 cgccgaacca acggccaggc cagcaacgaa ctgcgccacg gtaacgtcat gctcgacccc
    45781 ggcaaacgta tcgccacgct ggctggcgaa cccttaactc tgaaaccaaa agaatttgcc
    45841 ctgctggaat tactgatgcg taacgctggt cgggtactgc cgcgcaaact gattgaagag
    45901 aaactgtata cctgggacga agaggtcacc agtaatgccg ttgaagtgca tgtgcatcat
    45961 ctgcgacgca aactcggcag tgattttatt cgtaccgttc atggtattgg ctacacatta
    46021 ggtgagaaat gaaatttacc caacgtctta gtctgcgagt caggctgacg ctaatctttt
    46081 taattctggc ctcagtgacc tggctgcttt ccagctttgt cgcctggaaa caaacaacgg
    46141 ataacgtcga tgagttgttc gacacccaac tgatgctgtt tgccaagcgg ttaagtacgc
    46201 tcgatctcaa cgaaatcaac gcggcagatc gcatggcaca gacgccaaat aaattaaaac
    46261 acggtcatgt tgatgacgat gcgctaacct ttgccatctt tacccacgac ggcagaatgg
    46321 tccttaacga tggcgataac ggagaagata ttccctatag ctatcaacgg gaaggttttg
    46381 ctgacgggca actggtcggt gaagacgatc cctggcgttt tgtctggatg acctcacctg
    46441 atggcaaata tcgcatcgtt gttggtcagg aatgggaata ccgcgaagac atggcgctgg
    46501 cgattgttgc agggcaattg atcccgtggc ttgtcgcact gccgattatg ttaatcatca
    46561 tgatggtact actgggtcgt gaactcgcgc cgctgaacaa actggcgctg gcactacgta
    46621 tgcgtgaccc tgactcggaa aaaccactaa acgcgacggg cgtacccagc gaagtgcgtc
    46681 cactggttga gtcgttgaat caactgttcg cccgcacaca tgcgatgatg gttcgagaac
    46741 gacgctttac ctccgacgca gctcacgaac ttcgtagccc gttaacggcg ctgaaagtgc
    46801 aaaccgaagt tgcgcagctc tctgacgatg atccgcaggc gcggaaaaaa gcgctgcttc
    46861 aattacattc cggaatcgat cgtgctactc gtctggttga tcaactgctc acgctatcgc
    46921 ggctggactc actggataac cttcaggacg tcgcggagat cccgcttgaa gatctcctgc
    46981 aatcatcggt gatggatatt taccacacgg cgcagcaggc gaaaattgac gtgcgactga
    47041 cactcaatgc ccacggcatc aaacgcaccg gacaaccgct gttgctaagt ttgttggtac
    47101 gaaaccttct ggataacgcc gtgcgctaca gtccacaggg cagcgtggtg gacgtcacgc
    47161 tgaatgccga tagtttcatc gtgagggata acggccccgg tgtgacgcca gaggcactgg
    47221 cgagaattgg cgaacgcttc tatcgcccac ccggacaaac cgctaccggc agtggacttg
    47281 ggctatcgat tgtccagcga atcgccaaat tgcatggcat gaatgttgag tttgggaatg
    47341 cggaacaagg tggatttgag gcgaaggtga gctggtaact tctctgtcta tgagagccgt
    47401 taaaacgact ctcatagatt ttactattag caaaatataa accgtcccca aaaaagccac
    47461 caaccacaaa ccaaacaaat aacgagcgtg aatacatata gccaaaactc gaacatttcc
    47521 cgcataaaga gttttcttaa gataagaata ataagtggcg tcagaagaaa aaatgctgca
    47581 atcattgctt gcaatatacg atcttcgcct gaaacggaag gtatactgca aatattgtct
    47641 cccgactcaa gcatccagtc atacttattg aaagcaaaaa aagcatctat taaccaataa
    47701 gtaagcatta tatataacag agcttcaaat attgt
//
LOCUS       DACBVI010000018        46873 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.018, whole genome shotgun sequence.
ACCESSION   DACBVI010000018 DACBVI010000000
VERSION     DACBVI010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.018"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            164..1519
                     /locus_tag="IU325_05210"
     CDS             164..1519
                     /locus_tag="IU325_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HAP5808441.1"
                     /translation="MARFQFKSRKNNGFIFLISFMVMGEATIAAPLPQWANAPAVTPV
                     AQLSLQESILRAFARNPDVTQQAAQIGIGEAQIDEAKSAWYPHVGLTGNAGPSRQTDS
                     SGRLDNNVSYGITLTQLVYDFGKTNNDIKLQTAARDSYRFKLMATLTDVAEKTATAYM
                     EVSRYQALCDAAQRNIHSLENVYNMAALRANAGLNSSSDELQAQTRIAGMRSTLEQYQ
                     AQMASAKAQLAVLTGVQPEAIAAPPAELAEQPVSLKNIDYQSIPLVLAAENLRQSAQY
                     GVEKTKAQYWPTLSIQGGKTRYQTSDRSYWDDQLQLNVNAPLYQGGAVSAQVQQAEGQ
                     QKISASQVEQAKLDVLQRASVAYANWTGARGREEAGLAQSESAHRTRDVYQNEYKLGK
                     RSLNDLLTVEQDVFQAQSAEINANYDGWVAAVNYAAAVNNLIPLAGIKQGLYNDLPDL
                     K"
     gene            1628..22402
                     /locus_tag="IU325_05215"
     CDS             1628..22402
                     /locus_tag="IU325_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HAP5808442.1"
                     /translation="MSLIIDVISRKTSVKQTLINPGDVTVVIYEPSVVQVHAQASAVA
                     RYVRDGNDLLIYMQDGTVIRCNGYFLQAANTSEQSELVFVDGQQLTHVTFADTAAGGL
                     APVELTAQTTAIESIAPYLDIVGQTTAFPWGWLAGAAVGGGALGALLASGGDDDSKTE
                     VVNNPPPAEPGNATPSFLVTDNQGDQRGILSANDTTDDTTPTFSGSGQAGATIQIKDS
                     NGDTIASTQVGSDGRWSVDLPTQSAGEHTWSVVQIVGSTITDAGSITLTIDNSQASVQ
                     VATTAGDNIINASEQAAGFTLSGTSSHLAQGTELTVTLNGKTYTTSVGANGAWSVQVP
                     TADAQALGEGNQAVLVSGKDATGNTVTGVQLLTVDTQPPTLAINTIAQDNIISAAEHN
                     VALVLSGTSNAEAGQTVTLTVNGKSHTATVGSDGTWQVTLPATEVQALAEGNYAVNAS
                     VSDRAGNSTSHSANFTVDTSAPVVSVNTVAGDDILNNAEQAVAQIISGQVSGASPGDT
                     VTVKLGTHVLTGIVLADGSWNVALDPAVTRTLDRGANTIFVTVTDTAGNTGAASRAIT
                     LVGVSPLITINTVSGDDIISGAEKGAPLTLTGSTQQAETGQTVTVTLAGQSFTTTVQA
                     DGSWSLTVPAAAMGNLPDGAVAITASVTDLSGNTGNTSRTITVDSQAPALSIDPLTAD
                     NIINAAESGQDLPITGTTDAQPGQTVTVTLNGQTYQGVVQPDGTWSVTVPAANVGALA
                     DGNATVTASVNDIAGNPTSVSRVALVDATPPVVTINPVATDNVINTPEHTQAQIISGT
                     VTGAQAGDIVTVTLNNVDYTTVVDGSGNWSLGVPASVVSGLVDGSYPINVSVTDRAGN
                     TGSQSLTVTVNTAAPLIGINSIAGDDVINASEKGADLQITGTSDQPVNTAITVTLNGQ
                     NYTTTTDASGNWSVTVPASAVTALGQANYTVTAAVTSNIGNSNTASHNVLVDSALPGV
                     TINPVATDDIINAAEAGVAQTISGQVTGAEDGDTVTITLGGNTYTATVGSNFTWSVSV
                     PAADIQALGNGDLTVSASVTNQNGNTGSGTRDITIDANLPGLRVDTVAGDDVVNIIEH
                     GQALVVTGSSSGLAEGTPLTVTINNVEYTTAVQADGSWSVGVTAAQVSTWPAGTVNIA
                     VSGESSAGNSVSITHPVTVDLTPAAIAINTIATDDVINAAEKGADLTLSGTTTNVEPG
                     QTVTVTFGGKNYTASVASDGSWTATLPAADLTALPEGSASAQASVSNINGNSASAVHN
                     YSVDSSAPTIIINTVASDNIVNASEADAGVTVSGSTTAEAGQIVTVTLNSPTVQTYQA
                     TVQADGSWSINIPAADLEALTDGSHTLTATVNDKAGNPASTTHNLAVDLTVPVLTINT
                     IAGDDIINAAEHGQALVISGSSTGGEAGDVVTVTLNSKTYTTTLDASGNWSVGVPAAD
                     VTALGSGPQTVMATVTDAAGNSDSETHTVTVNLTAPTIGINTIATDDIINATEKGADL
                     QISGTSNQPAGTTITVTLNGQNYTATTDASGNWSTTVPASAVGALGEASYTVTANVTD
                     SAGNSNSASHNVQVNTALPGVTINPVASDDIINAAESGVAQTISGQVTGAAAGDTVTV
                     TLGGKTYTATVQGNLSWSVDVPAADIQAIGNGDLTVNASVTNGVGNTGSGSRDITIDA
                     NLPGLRVDTVAGDDVVNSIEHGQALVITGSSSGLAAGAALTVVINNVTYGATVLADGT
                     WSLGVPAADVGNWPAGTVNITVSGTNTAGTTTTITHPVTVDLAAVAISINTVSGDDVI
                     NAAEKSADLTLSGSTSGVEAGQTVTVTFGGKTYTATVAGDGSWTTTVPAADLSALRDG
                     DATVQASVSTINGNTASATHAYSVDATAPTLAINTIATDDILNAAEAGNPLTISGSSN
                     AEAGQTVTVTLNGVTYTGTVQADGSWSVSVPTADLSNLTASPYTVSASVNDKAGNPAT
                     ATHGLAVDLTVPVLTINTVSGDDIINAAEHGQALVISGSSTGGEAGDVITVTLNSKTY
                     TTTLDASGNWSVGVPAADVTALGSGPQTITATITDIAGNSDDASRTVTVNLTAPTIGI
                     NTIASDDVINATEKSADLQITGTSNQPAGTTITVTLNGQNYTATTDSSGNWSATVPAS
                     AVSALGEASYTVTANVTDSAGNSNSASHNVLVNSALPAVTINAVATDDIINAAESGNA
                     QTISGQVTGAAAGDTVTVTLGGNTYTATVQANLSWSVSVPAADIQAIGNGSLTVNASV
                     TNVVGNTGNGSRDITIDANLPGLRVDTVAGDDVINSIEHNQALVITGSSSGLTAGTAL
                     TVEINNVTYGATVLADGTWSLGIPAADVSNWPAGTVDITVSGTNSAGTTSTITHPVTV
                     DLAGVAITINTLSGDDVINAVEKGETLVVSGSTSGVEAGQTVTVTFGGKNYTTTVESN
                     GSWTVNVPPADLAALPDGAGNVQASVSNINGNSAQADRAYSVDATAPLVTINTIASDD
                     ILNVSEAGAGITISGTTTAQVGQTLTVTLNNNTYQTTVQADGTWSVNVPATDLSGLTA
                     SSYTVTATVSDKAGNPASADHALAVDVTAPDLTINTVAGDDIINAIEHGQALVVSGTS
                     TGAAAGDVVTVTLNGKNYTTTLDASGNWTVGIPAADVTALATGSQTITASLSDRAGNS
                     DSTTHNVTVDLSGPTLTISTVSDDDIINSTEKTQDLTISGGSSGLATGTTVTVMLNGL
                     AYSATTDSSGNWSVTVPASAVGALGEAVYQISASATDSAGNNGSTTHTVNVESLLPGV
                     IINTVAGDDIINAAEIAVAQTISGQVTGTAVAGNTVIVTIGGNQYTATVQPDLSWSVS
                     VPANVLQALGNGELTISASVTNSANNTGTATHDIVIDANLPGLRVDTVAGDDVINSIE
                     HTQALVITGSGSGLAAGAALTVVINSVTYGATVLADGTWSVGVPAADVTNWPAGTVNI
                     AVSGTNTAGTTTSISHPVTVDLAAVAITINTLSTDDVINAAEKGSDLQLSGTTSDVEA
                     GQTITVIFGGKSYTTTVAAGGTWGLTIPAADLATLPDGAANVQASVSNVAGNSAQATH
                     AYSVDATAPSVTINTIASDDILNAAEAGSALTISGTSTAEAGQTVTVTLNGVNYSGNV
                     QADGSWSVSVPTGDLANLTASPYTVSAAVSDKAGNPASATHNLTVDLAAPVVTINTVA
                     GDDIINATEHAQAQIISGSATGATTGNTVSVTIGTTTYTTVLDANGNWSIGVPASVIS
                     ALAQGDVTITATVTDSAGNSGTASHTVTVALGAPVLAINTIAVDDIINATEKGADLAI
                     SGSSNQPAGTQITVTLNGQNYTTTADASGNWSVTVPASRVSALGEATYTVTAAATDSD
                     GNSGSASHNVQVNTALPGVTINVVATDDIINAAEAGVDQTISGQVTGATAGDTVTVTL
                     GGATYTATVQANLSWSVDVPAAALQALGNGELTISASVTNSVGNTGNGTREITIDANL
                     PGLRVDTVAGDDVVNIIEHGQALVITGSSSGLATDSNVTLTINGQTYVAAVLADGSWS
                     VGVPAADVSAWPAGTVTITASGSTTAGNPVSVTHPVTVDLTAVAVSINAITADDVINA
                     AEKGAALTLSGSTSGVEAGQTVTVTFGGKTYTASVAANGSWSTSVPAADMAALRNGDA
                     SAQASVSNVNGNNATTTHAYSVDASVPTVTINTIAGDDILNAAEAGAALTITGSSTAE
                     AGQTVTVTLNGANYTGTVQTDGSWSISVPPADLSALTASNYTVSAAVSDKAGNPASVN
                     HNLTVDTSVPVVTINTVAGDDVINATEHAQAQIISGSATGAATGSTVTVTIGTNTFTT
                     VLDASGNWSVGVPASVVSALANGTVTINASVTDAAGNSGSATHQVTVNTGLPSITFNA
                     ISGDNVLNADEKGQPLTISGSSTGLATGAQVTVTLNGHNYSATTDAAGNWTLTVPVSD
                     LAALGQANYIVSASATSAAGNTASSQANLLVDSGLPGVTINTVAGDDIINAAEAGAAQ
                     TISGVVTRAAAGDTVTVTLGGNTYTAQVQADLSWSVSVPAADLQALGNGDLTITASVT
                     NANGNTGSGTRDITIDANLPGLRVDTVAGDDIVNSIEHGQALVITGGSSGLNAGVPLT
                     ITINGTAYSATVQADGSWSVGIPAANVSAWPAGALTVEVDGQSSAGNPVGVSHPFTVD
                     LTAVAISISTVASDDVINAAEKGTNLTLSGSTSGIESGQTVTVTFGGKTYTASVAANG
                     SWSVNVPAADLAILPDGAANVQASVSSASGNSASATHAYSVDASAPTLTINTIASDDI
                     LNATEAGSPLIISGTSTAETGQTVTVTLNGATYSGNVQADGSWSVSVPPSALGALSAS
                     NYTVSATVNDKAGNPGSASHNLAVDTTAPVLTINTVVGDDIINDAEHAQALVISGTST
                     GGEAGDVVSVVLNGKTYTTTLDASGNWSVGVPAADVAALGSGAQTITASVSDRAGNSD
                     DASRTVTVSLTAPVISINTIAGDDVINATEKGSDLALSGISDQPAGTAITVTLNGQNY
                     SATTDSSGNWSVTVPASAVSALGEASYSVTASVTNAQGNSSTASHNVQVNTALPGVTI
                     NPVTTDDIINAAEAGSAQTISGQVTGAAAGSTVTVELGGKTYTATVQADLSWNVSVPA
                     ADWQALGNGELTVNASVTNAVGNTGSGTRDITIDASLPGLRVDTVAGDDVVNIIEHSQ
                     AQVITGSSSGFAAGTALTVVINNQTYAATVLANGSWSVGVPATDVSNWPAGTLNITVS
                     GANSAGTQTSITHPLTVDLTTVAVSINSITSDDVINAAEKGAALTLSGSTSGVEAGQT
                     VTITFGGKTYTTTVAANGSWSTTVPAVDMATLRDGDASAQVRVTNVNGNSATATHEYS
                     VDSAAPTVTINTIASDNIINASEAAAGVTVSGTSTAETGQTLTVTLNGTNYQTTVQAD
                     GSWSLTLPASDLTALANNGYTLTASVSDLAGNPGSASKGVTVDTTAPVISFNTVAGDD
                     VINNVEHTQAQIISGTATGAVAGDRLVVTIAGQQYVTSTDASGNWSVGVPASVISGLA
                     DGTVTISATITDSAGNSSTQTHNVQVNTAAVSLSVSTISGDNIINAAEAGSALTLSGT
                     GTNFAAGTVVTVLLNGKGYSATIQSNGSWSVNVPAADVAALSDGTSYTVSASAQDSAG
                     NSATASRSVAVDLTAPVISINTVSTDDRLNAAEQQQPLTLNGSTSAEVGQTVTVTFGG
                     KTYTATVAANGTWALNVPAADLAALGQGAQTITASVNDRAGNPGQTTHALTVDTVAPT
                     VTIATVAGDDIINNAEQLAGQTISGTTTAEVGQTVTVTFNGQSWTATVGSGGSWSVFI
                     PAQQFAGLSDGSYTISATVSDQAGNPGSASRGVTLNGDVPSVTINTFAGDDVVNAAEH
                     GSSLVISGTTTAPVGQTLTLTLNGKTYTTTVQTGGSWSYTLGSVDVTALADGNAYVIN
                     ASVSNAIGNTGSSNHTITVDLSAPAMGINIDSLQADTGLSSSDFITSVSPVVVNGSLT
                     AALASNETAQISIDGGVTWTTLTVTGTTWRYNDSRTLTDGSYLYQVRVIDAAGNVGAT
                     DSQNVVIDTIAPDPAVKTIAINAITTDTGLITNDFVTSDTTLAVSGTLGAALSSGEFA
                     QISIDGGTTWQNLSVSGLTWTYLDGRTLSDGNYNYQVRVIDTAGNIGATASQIVTVDT
                     TAPLASKTIAIAGISDDTGLSSSDFVTRDTTLTVRGTLGAALAADERAQISLDGGVTW
                     TTLTVIGTSWSYADGRTLTDGTWNYTVRVVDLAGNVGQTATQNVVVDTTSPEAAKSIT
                     ITGISDDTGASSSDFITSDTSLTVRGVLGAALGANEFAQISTDNGATWVNVTLAADGL
                     NWSYVDGRTLTNGTTTWQVRVVDLAGNVGATSSQSAQIDTVNPAQVLTIASISTDTGS
                     SATDFITSDTTLTLTGSLGAGLASGEVAQISLDGGATWITLTTNGTQWTYTDSRTLTD
                     GSYVYQVRVLDLAGNTGPVVSKTVVVDTINPTATPTIVSYTDDVGQRQGTLSSSQATD
                     DTTPLLNGVLSGPLASGEVVYLYRNGLLLGAVTMVGALNWTYSDSGLVSGAYTYSARV
                     VDLAGNITASSDFVLTVDTSIPTTLAQITSQTTRDTTPIISGVITAALASGQYVEVVI
                     NGKTYTSEPGGAVVVDPAHNTWYVQLPDTDALAASATAYNVTAQVKSSAGNGNNANIS
                     NGTVTVNAAIDYTPTWTTASKTTAWGLTYGLDSHGMWTVLANQQVMQSTDPLTWSKTA
                     LTLYQSGNNYATSSIADYDRNGTGDLFITRDDYGTGYINGFTNNGDGTFSSAIQVTVG
                     TLTWYGSIVAFDKEGDGYLDFWIGDAGGPDSNTFLWNNAGTLVGNSTTSNSGGSATVG
                     GAVTGYLSLNEGSGVDLNNDGRIDLVQHTYNLNNYYTLSSLINQGNGTFVWGQNTTNT
                     FLSGAGSGAMSSSVSMTWADFDGDGDMDLFLPASQGRANYGSLLFNTNGVLGSPVAVG
                     ATATTYASQFSVAVDWNHDGLMDIARIAQTGQSYLYTNVSNASNWTQSALGGSQSGTT
                     SGVAAMDYDWDGAVDVLVTKQSGSVYLIRNTNTVSYGTSLHLRITDPNGINVYYGNTV
                     KLYNSAGVLVATQIINPQSGMGVNDTSALVNFYGLNAGETYNAVLIKSTGTTASNIDQ
                     TVNTSWGGLQATDATHAYDLSAEAGTASNNGKFVGTGYNDTFFATAGTDTYDGSGGWV
                     YSSGTGTWLANGGMDVVDFRLSTVGVTANLSSTAAQATGFNTSTFTNIEGISGSNFND
                     TLTGSSGDNQLEGRGGNDTLNIGNGGHDTLLYKLLNASDATGGNGSDVVNGFTVGTWE
                     GTADTDRIDIRELLQGSGYTGNGKASYVNGVATLDAQAGNIGDFVKVTQSGSDTIVQI
                     DRDGTGGTFAATNVVTLTGVHTDLATLLANHQLMVV"
     gene            22406..24568
                     /locus_tag="IU325_05220"
     CDS             22406..24568
                     /locus_tag="IU325_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HAP5808443.1"
                     /translation="MKKNAQSVEAWLEAMIAVARYYRLDFSQENVRATVNWERDSKRE
                     ELLTDMARQLGMGLRLVEFSTDSLNPWRLPLIAVFDNQQIGVITRRDNHDNISVQFSG
                     DEGLETTLNVTDIEDNIVELALLRPLSAIPDARVDDYIRPYQANWFWNLSLKDWRRYG
                     DIMLASLVANVLALAAMIFSMQVYDRVVPAQSYPTLWVLFAGVMMAILFEFCMRMVRT
                     HLSDVIGKRADLRISDRVFGHALRLKNNVRSKSTGSFISQIRELESVRELITSTTIGA
                     VADLPFFLLFVFILWMIGGWLVLVVLLALPLLVIPGLLVQRPLARLANEGMRESAVRN
                     ATLVEAVQSIEDIKLLRAEQRFQNQWNHTNDVASSISMKQRFLTGLLLTWTQEVQSIV
                     YVVVLLVGCFMVMNGDMTTGALVGTTILASRTIAPLSQISGVLSRWQQAKVARNGLDE
                     LMKRPVDQPEHGKLVHKAVLHGNYQFSNAIFYYDEEEKIADVAIGKLNIQAGEKIAIL
                     GRNGAGKSTLLQMLAGMRIAQQGQVLLDNISIGQLDPADLRRDMGLLSQTGRLFFGSL
                     RENLTMGMPEASDEDIERALTLSGALPFVQKQKNGLNYMIQEGGFGLSGGQRQTLLLA
                     RLLISQPNIVLLDEPSASLDEMAEAYLIEQLKQWIGHRTLIIATHRTAMLQLVDRIIV
                     MDQGRIVMDGAKEAILREQGEPTTRRVVLQEKNKGSAA"
     gene            24565..25740
                     /locus_tag="IU325_05225"
     CDS             24565..25740
                     /locus_tag="IU325_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HAP5808444.1"
                     /translation="MNGQLQYNRGLTEPRLPRSALAVRVTAVMLLCFLGWAWYFQLDE
                     VTTGSGTVEPSGREQVVQSLEGGILYHLDVKVGDIVEQGQPLAQLNRTKTESDVQEAM
                     SRLYAALATSARLRAEVSNKPLVFPDELNKFPELIESETALYNTRRDGLNKATTGLTQ
                     GISLVNRELAMTQPLVKQGAASSVEVLRLQRQANELENKLSDVRTQYYVQAREELAKA
                     NAEVETQRSVIRGREDSLTRLNFTAPVRGIVQDIDVTTVGGVIAPGGKLMTIVPLDEQ
                     LLIEAKISPRDVAFIHPGQKSLVKITAYDYSIYGGLPGEVAVISPDTVQDEVRRDVYY
                     YRVYIRTFSNHLENKSKQQFPIFPGMVATVDIRTGKKSVLDYLLKPFNKAQEALRER"
     gene            25737..26144
                     /locus_tag="IU325_05230"
     CDS             25737..26144
                     /locus_tag="IU325_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CueR"
                     /protein_id="HAP5808445.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(26348..26770)
                     /locus_tag="IU325_05235"
     CDS             complement(26348..26770)
                     /locus_tag="IU325_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808446.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPTSHIADCQAQGVSHDTCYLAE
                     QQRQTAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNKDATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            complement(26824..27840)
                     /locus_tag="IU325_05240"
     CDS             complement(26824..27840)
                     /locus_tag="IU325_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAP5808447.1"
                     /translation="MKTVNVALLALIISATSSPVVLAGDTIEAAATELSAINSGMSQS
                     EIEQKITRFLERTDNSPAAYTYLTEHHYIPSETPDTTQTPTVQTDPDAGQKTVAATGD
                     VQTTARYQSMINARQSAVTDAQQTQITEQQAQIVATQKTLAATGDTQNTAHYQEMINA
                     RLAAQNEANQRTTTEQGQKMNALTTDVAAQQQKERAQYDKQMQSLAQKSVQAHEQIES
                     LRQDSAQTQQQLTNTQKRVADNSQQINTLNNHFDSLKNEVEDNRKEANAGTASAIAIA
                     SQPQVKTGDLMMVSAGAGTFNGESAVSVGTSFNAGTHTVLKAGISADTQSDFGAGVGV
                     GYSF"
     gene            28207..28569
                     /locus_tag="IU325_05245"
     CDS             28207..28569
                     /locus_tag="IU325_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808448.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKNMNKGSDE"
     gene            complement(28754..29212)
                     /locus_tag="IU325_05250"
     CDS             complement(28754..29212)
                     /locus_tag="IU325_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HAP5808449.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLSAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(29209..30126)
                     /locus_tag="IU325_05255"
     CDS             complement(29209..30126)
                     /locus_tag="IU325_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HAP5808450.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            30272..30949
                     /gene="fetA"
                     /locus_tag="IU325_05260"
     CDS             30272..30949
                     /gene="fetA"
                     /locus_tag="IU325_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HAP5808451.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            30936..31715
                     /gene="fetB"
                     /locus_tag="IU325_05265"
     CDS             30936..31715
                     /gene="fetB"
                     /locus_tag="IU325_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HAP5808452.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(31778..32632)
                     /gene="cnoX"
                     /locus_tag="IU325_05270"
     CDS             complement(31778..32632)
                     /gene="cnoX"
                     /locus_tag="IU325_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HAP5808453.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(32693..33502)
                     /gene="ybbO"
                     /locus_tag="IU325_05275"
     CDS             complement(32693..33502)
                     /gene="ybbO"
                     /locus_tag="IU325_05275"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAP5808454.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(33492..34115)
                     /gene="tesA"
                     /locus_tag="IU325_05280"
     CDS             complement(33492..34115)
                     /gene="tesA"
                     /locus_tag="IU325_05280"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HAP5808455.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            34086..34772
                     /locus_tag="IU325_05285"
     CDS             34086..34772
                     /locus_tag="IU325_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP5808456.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            34769..37183
                     /locus_tag="IU325_05290"
     CDS             34769..37183
                     /locus_tag="IU325_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP5808457.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLSLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIIAGNW
                     PPKADEVSMEEGLAKRLNVGLGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLHTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(37896..38990)
                     /gene="mnmH"
                     /locus_tag="IU325_05295"
     CDS             complement(37896..38990)
                     /gene="mnmH"
                     /locus_tag="IU325_05295"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HAP5808458.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVDGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLHLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDVQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            complement(39059..39985)
                     /gene="allS"
                     /locus_tag="IU325_05300"
     CDS             complement(39059..39985)
                     /gene="allS"
                     /locus_tag="IU325_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HAP5808459.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            40215..40697
                     /gene="allA"
                     /locus_tag="IU325_05305"
     CDS             40215..40697
                     /gene="allA"
                     /locus_tag="IU325_05305"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HAP5808460.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQKRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            40775..41590
                     /gene="allR"
                     /locus_tag="IU325_05310"
     CDS             40775..41590
                     /gene="allR"
                     /locus_tag="IU325_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HAP5808461.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            41680..43461
                     /gene="gcl"
                     /locus_tag="IU325_05315"
     CDS             41680..43461
                     /gene="gcl"
                     /locus_tag="IU325_05315"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HAP5808462.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERKVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAVDAPTETCFMHYE"
     gene            43474..44250
                     /locus_tag="IU325_05320"
     CDS             43474..44250
                     /locus_tag="IU325_05320"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HAP5808463.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            44350..45228
                     /gene="glxR"
                     /locus_tag="IU325_05325"
     CDS             44350..45228
                     /gene="glxR"
                     /locus_tag="IU325_05325"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HAP5808464.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            45398..46852
                     /locus_tag="IU325_05330"
     CDS             45398..46852
                     /locus_tag="IU325_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HAP5808465.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAENTGEQKVTG"
BASE COUNT        10906 a        12746 c        13212 g        10009 t
ORIGIN      
        1 cgtagtgtat tctaaataaa taacgtataa gctttttagc tgcatttctt tgccaatatc
       61 aatgatcgac aaaagagatg cacaagttat tacccttctt aataaaatga gactagttat
      121 aaaaaccagt taaaataatg acctggaagg aagtgactta tctatggcgc gattccagtt
      181 taaaagccgt aaaaataatg ggtttatttt tttaataagt tttatggtga tgggagaagc
      241 tacaattgct gcaccgctgc cacaatgggc gaacgcccca gcggtaacgc cagttgccca
      301 attatcttta caggaaagta tattacgcgc ctttgcgcga aatcccgacg tcactcaaca
      361 ggcggcgcag ataggtattg gcgaagcgca aattgatgaa gccaaaagtg cctggtatcc
      421 gcacgttgga ttaaccggca atgcgggacc gtcccgacaa acggactcca gtggcaggct
      481 tgataacaac gtttcgtatg gcataaccct gacacagctg gtgtatgact ttggtaaaac
      541 taacaacgat atcaaactgc aaactgccgc ccgtgacagc taccgcttta aattgatggc
      601 aaccttaacc gatgttgcag agaaaacggc gactgcctat atggaagtca gtcgttatca
      661 ggctttgtgc gatgcggctc aacgcaatat tcactcgctg gaaaacgtct acaacatggc
      721 agcattgcgt gctaacgctg gcctgaactc gtcgtcggat gaattacagg cccagacgcg
      781 cattgccgga atgcgctcaa cgctggagca atatcaggcg cagatggcaa gcgccaaagc
      841 gcaactggcg gtgctcactg gcgtacagcc ggaggcgata gccgcgccac ctgctgaatt
      901 agccgagcag ccggtatcgc tgaaaaatat tgattaccag tccatcccgc tggtgctggc
      961 ggcagaaaac ttacgccagt cagcacagta cggcgtggaa aaaacgaaag cgcaatactg
     1021 gccaacgctg agtattcagg ggggcaaaac gcgctaccag accagcgacc gctcgtattg
     1081 ggatgatcag ctacaactga acgttaacgc gccgctgtat cagggcggcg cggtttctgc
     1141 ccaggtgcaa caggctgaag ggcaacaaaa aatctctgcc tcgcaggtcg aacaggcaaa
     1201 actggatgtg ctgcaacgag cgtctgtggc atatgccaac tggacaggcg cacggggtcg
     1261 tgaagaggct gggttagcgc aatctgaaag tgcgcacaga acgcgagatg tctaccaaaa
     1321 tgaatataaa ttgggtaaac gcagcctgaa tgatctatta accgtcgagc aagatgtctt
     1381 tcaggcgcaa tcggctgaaa taaatgccaa ttatgatggc tgggtcgccg cagtgaatta
     1441 tgccgctgcg gttaataacc ttattccatt agcgggaatt aaacagggct tatacaacga
     1501 cttacccgac ttgaaataat cctgccatta ttaatatcag gtatgcatcg ttgttgagtg
     1561 attacctttg tcttatttag ataaatggac tcgctaagga tgtgcgccat ttatcaggaa
     1621 tgccattatg agcctgatta ttgatgttat ttcgcgaaaa acatccgtca aacaaacgct
     1681 gatcaatcct ggcgacgtta cggttgtcat ttatgagccc tctgtggtgc aggttcatgc
     1741 tcaggcatct gccgttgcgc gttatgttcg tgacggaaat gacttgttga tctatatgca
     1801 ggacggcacg gtgatccgct gcaacggtta tttcctgcaa gcggcaaata ccagcgaaca
     1861 atccgaactg gtgtttgtcg atggtcaaca actgacccac gtaacatttg ccgataccgc
     1921 ggcaggtgga ttagcgcccg tggagctgac cgctcagacc accgcaattg aaagcattgc
     1981 gccgtacctt gatatcgtcg gccagacgac ggctttcccg tggggatggc tggcgggggc
     2041 ggcggtaggt ggcggcgcgc ttggcgcttt actggcaagc ggtggcgatg atgactcaaa
     2101 aactgaagtg gttaacaacc caccgccagc cgaacccggc aacgccacgc catcgttttt
     2161 ggtaaccgat aatcagggcg atcaacgcgg tattctttcc gccaacgata ccaccgatga
     2221 cactacgcca acctttagcg gcagcgggca ggcgggggcg accatccaga ttaaagacag
     2281 caatggtgac accatcgcca gtactcaggt cggtagcgat ggacgctgga gcgtcgattt
     2341 gcccacgcaa agcgcgggcg aacatacctg gtcagtggtg caaatcgtcg gcagtaccat
     2401 cactgacgcc ggttcgataa cgttaaccat cgacaatagt caggccagcg tgcaggttgc
     2461 caccaccgca ggcgataaca ttattaacgc cagcgaacag gccgccgggt ttacgctttc
     2521 tggcaccagt agtcatctgg cacagggaac agaactcacc gttacgctaa acggcaaaac
     2581 ctacaccacc agcgtaggcg ctaacggtgc ctggagcgtg caggtaccga ccgccgatgc
     2641 gcaggcgtta ggcgaaggga atcaggcagt gctggtcagc gggaaagacg ccacaggcaa
     2701 taccgtcacc ggcgtgcaac tgctaacggt cgatacccaa ccgccaacgc ttgccatcaa
     2761 caccatcgct caggacaaca tcatcagtgc tgcggaacat aacgtcgcgc tggtactgag
     2821 cggcacgtcg aatgcagaag cggggcaaac cgtaacactg accgtcaacg ggaaaagcca
     2881 tacagcaacc gtcggtagcg acggaacctg gcaagtgacg ctgcctgcca cggaagtcca
     2941 ggcgctggcg gagggtaatt acgctgtcaa tgccagtgtc agcgatcggg cagggaacag
     3001 caccagccac agcgcgaatt tcacggtaga tacctcagca cccgtggtca gtgttaatac
     3061 cgtagcgggc gacgatattc ttaataatgc tgagcaggcc gtcgcgcaga tcatctccgg
     3121 acaagtcagc ggtgcttctc ctggcgatac ggtaacggtg aaattgggca ctcatgtcct
     3181 gacgggcatt gtgctggcag atggcagctg gaatgtggcg ctggacccag cggtaacccg
     3241 cacgctggat cgcggagcca atacgatttt cgtcaccgtg acagatactg caggaaatac
     3301 cggcgcggcg tctcgagcaa tcacgctggt cggtgtttct ccgttaatca ccattaacac
     3361 cgtctccggc gatgacatta tcagtggcgc agaaaaaggt gcgccactga cccttaccgg
     3421 tagcactcaa caggccgaga caggacaaac cgtcacagta accctggctg gacagagttt
     3481 taccactacc gtgcaggctg atggctcctg gagtctgacg gtacctgccg ccgcgatggg
     3541 aaatctgcct gacggcgcgg tggcgattac cgcttctgtg acggatctca gcggcaatac
     3601 cggcaacacc tcccgcacca ttaccgtcga cagccaggcc ccggcgttga gcatcgatcc
     3661 actgaccgct gataacatca ttaacgccgc cgaaagcggg caggatttgc ccatcaccgg
     3721 caccaccgac gctcagccag ggcagacggt gaccgttacg ttgaatgggc agacgtacca
     3781 gggcgtcgtg cagccagacg gcacctggag cgtgaccgtt cccgccgcca acgttggcgc
     3841 actggctgac ggcaacgcca ccgtcaccgc cagcgtgaac gatatcgccg gtaacccgac
     3901 cagcgtctca cgcgtggcgc tggtcgatgc cacgccgccg gtggtaacca ttaatccggt
     3961 ggcgaccgat aacgtcatca acacgccgga acacacccag gcgcaaatca tcagcggcac
     4021 ggttactggc gctcaggcgg gcgatatcgt caccgtgacg ctgaataatg tggattacac
     4081 aacggtggtg gatggttccg gcaactggag cctgggcgtt ccggcttccg ttgtcagcgg
     4141 actggtggat ggcagttatc cgatcaacgt gtcagtgact gaccgcgccg gaaacacggg
     4201 cagccagtca ttgaccgtca cggtcaatac tgccgcgccc cttatcggca ttaacagcat
     4261 tgcgggcgat gatgtgatta acgccagcga aaaaggggcc gatctccaga tcaccggcac
     4321 cagcgatcag cctgttaata ccgccatcac cgtgacgctg aacgggcaaa attacaccac
     4381 cacgaccgac gcctccggca actggagcgt caccgttccg gcatcggcgg taaccgcact
     4441 gggccaggcc aactacacgg taacggcggc ggtgacgagt aatatcggca acagcaacac
     4501 cgcaagccat aacgtgctgg tcgacagcgc gctgcccggc gtgaccatta acccggtggc
     4561 aaccgacgat attattaacg ccgccgaagc gggcgttgcg caaaccatta gcgggcaggt
     4621 tactggcgcg gaagatggcg acacggtaac tattacgttg ggcggtaata cttatacggc
     4681 gacggtgggc agcaatttca cctggagcgt gagcgttccg gcggcagata ttcaggcgct
     4741 gggtaatggc gatttaacgg tgagtgcctc agtcaccaat caaaacggca acaccggcag
     4801 cggcacgcgg gatatcacca tcgacgccaa tctgcccggc ctgcgggtcg atacggtggc
     4861 gggcgatgat gtggtcaata tcatcgagca cgggcaggcg ctggtggtca ccggcagcag
     4921 ttcggggctg gcagaaggca cgccgcttac cgtcacgatt aataatgtgg aatacaccac
     4981 cgcggtgcag gccgatggta gctggagcgt gggcgtcact gcggcgcagg ttagcacctg
     5041 gcctgcgggg acggttaata ttgctgtatc cggggaaagt agcgccggaa actcggtgag
     5101 cattacgcat ccggtgacgg tcgatctcac tccggcggcg atcgccatca acaccatcgc
     5161 cacggatgac gtgattaacg ccgcagaaaa aggcgctgat ttaacccttt ccggcaccac
     5221 cactaacgta gaacccggtc aaaccgtcac cgtcaccttt ggcgggaaaa attacaccgc
     5281 cagcgtagca agcgacggta gctggactgc caccttacct gccgccgatc tgacagcatt
     5341 accagagggc agcgcctccg cgcaggccag cgtcagcaat attaatggca atagcgcctc
     5401 ggcggtgcat aactacagcg tcgacagcag tgccccgacc atcattatca ataccgtcgc
     5461 cagcgacaat atcgtcaacg ccagcgaagc cgatgcgggt gtgacggtga gcggcagcac
     5521 caccgctgaa gcggggcaga ttgttacggt aacgctcaat agcccgaccg tgcagaccta
     5581 tcaggcaacg gtgcaggcgg acggcagctg gagcatcaat attccggcgg cagatcttga
     5641 ggcattgacc gatggcagcc acaccctgac cgccacggtc aatgacaaag cgggcaaccc
     5701 ggcgagcacc acgcataatc tggcggtgga tctcaccgtt ccggtactta ccatcaacac
     5761 cattgcgggc gacgatatta ttaacgccgc cgaacacggg caggcgctgg tgatttccgg
     5821 ttccagcact ggcggagaag cgggggatgt cgtcaccgtc acgctaaaca gtaaaaccta
     5881 caccacgacg ctggatgcct ccggcaactg gagcgtcggc gttccggcgg cggatgtcac
     5941 ggcgctgggt agcggcccgc aaaccgtgat ggcgaccgtt accgatgcgg caggtaatag
     6001 cgacagcgaa acgcacacgg tcaccgttaa ccttaccgcg ccaaccattg gcattaacac
     6061 catcgctacc gatgacataa ttaacgccac cgaaaaaggc gcggatctgc aaatttccgg
     6121 caccagcaat cagccagcgg gcaccactat tacggtgaca ctgaacgggc aaaattacac
     6181 cgccacgacc gatgcttccg gcaactggag caccacggtg ccagcctccg ctgtcggtgc
     6241 gctgggcgaa gccagttaca cggtgacggc gaacgtcacc gatagcgcgg gcaacagcaa
     6301 ttccgccagc cataacgtgc aggtcaatac cgcgctgcct ggcgtcacca ttaatccggt
     6361 ggcgagcgac gatattatta atgccgccga atcgggcgtg gcgcaaacta tcagtgggca
     6421 ggtgacgggc gcggcggcgg gcgataccgt caccgtgaca cttggcggga aaacttacac
     6481 cgctaccgtg caggggaatt taagctggag cgtggacgtt ccggcggcgg atattcaggc
     6541 catcggtaat ggcgatctca cggttaacgc ctcagtgacc aacggcgtgg gtaataccgg
     6601 cagcggttcg cgcgatatta ctatcgacgc caatttgcca ggtctgcgcg tcgataccgt
     6661 ggcgggcgat gatgtggtta acagcatcga gcacggtcag gcactggtga taaccggtag
     6721 cagcagcggg ctggcggcgg gcgcggcgct gacggtggtg atcaataacg tcacctatgg
     6781 cgcgacagta ttagccgatg gcacatggag ccttggcgtt ccggcggcag atgtgggcaa
     6841 ctggcctgcg gggacggtga atatcacggt aagcggcact aacaccgccg gaacaactac
     6901 gacaatcacc catccagtca ccgtcgatct ggcagcggtg gcgatctcca ttaacaccgt
     6961 ttccggcgac gatgtgatta atgccgctga aaaaagtgca gatttaaccc tttcaggcag
     7021 cacctccggc gtggaagcgg ggcaaacggt caccgttacc tttggcggga aaacctacac
     7081 cgctaccgta gcgggcgatg gtagctggac aaccaccgta cctgccgccg atctcagcgc
     7141 gttacgcgac ggcgacgcca cggtacaggc cagcgtcagc actattaacg gcaacacggc
     7201 ttcggcaacc cacgcctaca gcgtcgatgc cacggccccg acgcttgcca tcaataccat
     7261 cgccaccgac gatattctga acgccgccga ggcgggcaat ccgctaacca tcagcggcag
     7321 cagtaacgcc gaagcggggc agacggtaac cgtcacgctt aatggcgtga cttacaccgg
     7381 caccgtccag gctgacggaa gctggagcgt cagcgtaccg acggcggatc tcagcaatct
     7441 gaccgccagc ccgtacacgg tcagcgcctc ggtaaacgat aaagcgggta acccggcgac
     7501 cgccactcac ggcctggcgg tggatctcac tgttccagtg ctgaccatca acaccgtctc
     7561 cggcgatgac attattaacg ccgccgaaca cggacaggcg ctggtgatct ccggctccag
     7621 cactggcggg gaagcgggcg atgtcatcac cgtcacgtta aacagtaaaa cctataccac
     7681 cacgctggac gcttccggca actggagcgt cggcgttccg gcggcagatg tcactgcgct
     7741 gggcagcggc ccgcaaacca ttactgccac cattacggat atcgcgggta atagcgatga
     7801 cgccagccgc acggtcaccg tgaatctgac cgcgccaacc attggtatca acactatcgc
     7861 cagcgatgac gtgattaacg ccacggaaaa aagcgcggat ctgcaaatca ccggcaccag
     7921 taatcaaccc gcaggcacca caataacggt gacgctgaac gggcaaaatt acaccgcgac
     7981 gaccgattcc tccggcaact ggagcgccac ggtgccagcc tcggcggtta gcgcgctggg
     8041 ggaagccagt tacacggtaa ccgcgaacgt caccgatagt gcaggcaaca gcaattccgc
     8101 cagccataac gtgctggtca acagcgcgct gcctgccgtc accattaacg cggtggcgac
     8161 cgacgatatt attaacgctg ctgaatccgg aaacgcgcaa accatcagcg gccaggtcac
     8221 gggcgcggcg gcgggcgata cggtcaccgt tacgctgggc ggcaacacct acaccgccac
     8281 ggtgcaggct aatttaagct ggagtgtcag cgttccggcg gcagatattc aggccatcgg
     8341 caacggctct ctcaccgtaa acgcctcggt gaccaacgtc gtcggcaaca ccggcaacgg
     8401 ttcgcgagac atcactatcg atgccaatct gcctggcctg cgggtcgata ccgtggcggg
     8461 cgatgatgtt attaacagca ttgagcacaa tcaggcgctg gtgatcaccg gcagcagcag
     8521 cggattaacg gcgggaacgg cgttaacggt cgagattaac aacgttactt atggcgcgac
     8581 ggtattagcc gacggcacgt ggagcctcgg tattccggcg gcagacgtca gcaactggcc
     8641 tgcgggtacg gtggatatta cggtaagcgg caccaacagc gccggaacaa cctccaccat
     8701 tacccatccg gttaccgtcg atctggctgg ggtcgccatc accattaaca ctctctctgg
     8761 tgatgatgtg atcaacgccg tcgaaaaagg cgaaacgctg gtcgtaagcg gcagcaccag
     8821 cggtgtggaa gctgggcaaa cggtgactgt cacctttggt ggcaaaaatt acaccaccac
     8881 tgtggaatct aacggtagct ggacggtgaa tgtgccgcct gccgatctcg ctgcgctacc
     8941 ggacggcgcg ggcaacgtgc aggcgagcgt cagtaatatt aacggcaaca gcgcccaggc
     9001 ggatcgcgcg tatagcgttg atgccaccgc gccgcttgtg accatcaaca ccatcgccag
     9061 cgacgatatc cttaacgtga gcgaagcggg cgcagggatc accatcagcg gcaccaccac
     9121 ggcacaggtc gggcagacgc tgaccgtcac gctgaataac aacacgtacc agaccaccgt
     9181 tcaggcggat ggcacctgga gcgtgaatgt tccggcaacg gatttaagcg gattaaccgc
     9241 cagcagttat accgtgactg cgacggtgag cgacaaagcg ggcaacccgg caagcgccga
     9301 tcatgcgctg gcggtggacg ttaccgcgcc ggatctcacc attaataccg tcgcgggcga
     9361 tgacattatc aacgccatcg aacatggtca ggcgctggtg gtcagcggca ccagcacggg
     9421 cgcggcggcg ggtgatgtgg taaccgtcac gctcaacggc aaaaactaca ccaccacgct
     9481 ggacgcctcc ggtaactgga ctgtgggcat tccggcggcg gatgtcacgg cgctggcgac
     9541 cggtagccag acgattaccg ccagcctgag cgatcgcgcg ggcaacagcg acagcactac
     9601 ccataatgtg accgttgatc tcagcggccc gacgctgacc atcagtaccg tctccgacga
     9661 tgacattatt aacagtacgg agaaaaccca ggatctgacc atcagcggcg gctccagcgg
     9721 tctggcgact ggcaccacgg tcaccgtgat gttgaacggc ctggcgtaca gcgccactac
     9781 cgatagttcc ggtaactgga gcgttaccgt gcctgcttcg gcggtcggcg cactgggcga
     9841 ggcggtatac cagattagcg ccagtgccac cgacagcgcg ggcaacaacg gcagcaccac
     9901 ccatacggtg aatgtcgaat cgctcctgcc tggggtgatc attaatacgg tggcgggcga
     9961 tgacattatc aatgctgctg aaatcgctgt ggcgcagacc atcagcggtc aggtcacggg
    10021 aacagcggtt gcggggaata cggtgattgt cactattggc ggcaatcaat acaccgccac
    10081 cgtgcagcca gatttaagct ggagcgtcag cgtaccggcg aacgttttgc aggcgctggg
    10141 caacggagaa ctgaccatca gcgcctcggt gaccaattcc gcaaataata ctggcaccgc
    10201 aacgcacgat atcgtgatag atgccaacct gcctggcctg cgggtcgata ccgtggcagg
    10261 tgatgatgtg attaacagca ttgagcacac tcaggcgttg gtgatcaccg gtagcggtag
    10321 cggactggcg gcgggggcgg cgttgacggt ggttatcaat agcgtcacct acggcgcaac
    10381 ggtactcgcc gatggcacct ggagcgtcgg tgttcctgcg gcagatgtca caaactggcc
    10441 tgcgggaacg gtcaatattg ccgtttctgg caccaatacc gccggaacca caaccagcat
    10501 tagccatccg gtcacggtcg atctcgcggc cgtggcgatt accattaaca ccctttccac
    10561 tgacgatgtg attaatgccg ccgaaaaagg cagcgatttg cagctctccg gcaccacctc
    10621 cgatgtggaa gcggggcaaa ccatcaccgt tatcttcggc ggcaaaagct acaccaccac
    10681 ggttgccgcg ggcggcacct gggggctgac aatcccagca gccgatctcg caaccttgcc
    10741 tgacggcgca gcaaacgtcc aggccagcgt cagcaacgtc gcaggtaaca gcgcccaggc
    10801 aacgcatgct tacagcgtcg atgccaccgc gccatccgtt accatcaaca ccatcgccag
    10861 cgacgatatt cttaacgccg ccgaagcggg atcggcgctg accatcagcg gcaccagcac
    10921 ggcagaagcc gggcagacgg tgaccgtgac gcttaacggc gtcaattaca gcggtaacgt
    10981 gcaggcagac ggaagctgga gcgtcagcgt cccgaccggc gatttagcta acctcactgc
    11041 cagcccctat acggtgagcg cagcggtcag cgacaaagcc ggaaatccgg cttcagcaac
    11101 acataatctg acggtggacc ttgccgctcc ggttgtcacc attaacacgg tggcgggcga
    11161 tgacatcatc aacgccacag aacatgcaca ggcgcagatt atcagcggct cggcaacggg
    11221 cgcgactacc ggtaacacgg tttccgtgac cattggcacg accacctaca ccaccgtgct
    11281 ggacgccaac ggcaactgga gcatcggcgt gcctgccagc gtgatttccg cactggcgca
    11341 gggcgatgta accattaccg ccacggtcac cgactccgca ggcaacagcg gcacggcctc
    11401 gcacactgtc accgtggcgc tcggcgctcc ggtgctcgcc attaacacca tcgccgttga
    11461 tgacatcatt aatgccacgg agaaaggcgc ggatctggcg atttccggca gcagcaacca
    11521 gcctgcgggt acgcagataa ccgttacgct caacgggcaa aattacacca ccactgccga
    11581 tgcatccggt aactggagcg tgaccgttcc ggcgtcacgg gtgagcgccc tcggtgaagc
    11641 cacttacacg gtgaccgccg ccgctactga ctctgatggc aacagcggtt ccgccagcca
    11701 taacgtgcag gttaataccg cgctgccggg cgtcaccatt aacgtggtgg caacggacga
    11761 tattattaac gccgccgaag cgggcgtgga ccagaccatc agcgggcagg tgacgggtgc
    11821 tacggcaggc gacacggtga ccgtcacgct cggcggggcg acgtacaccg ccacggtgca
    11881 ggcgaattta agctggagcg tcgatgttcc ggcagccgct ttacaggcgc tgggcaacgg
    11941 cgagctgacc atttcggctt cggtaacgaa cagcgtaggt aataccggta acggcacgcg
    12001 tgaaattacc atcgacgcga atctgcccgg tctgcgggtc gataccgtgg cgggcgatga
    12061 tgtggtcaat attatcgagc acggacaggc gctggtgatt accggcagca gttccggtct
    12121 ggcgacggac agtaacgtca cgctgaccat taacgggcaa acctatgttg cggcggtact
    12181 ggcggatggc tcatggagcg tcggcgttcc ggcggcagat gtcagcgcct ggcctgcggg
    12241 gacggtcacg attacggcga gcggtagcac cacagccgga aatccggtca gcgttacgca
    12301 tccggtgact gtcgatctca ctgcggtggc ggtcagcatc aacgccatta ctgccgatga
    12361 cgtgattaac gctgccgaaa aaggcgcggc gttaacgctc tctggcagca cttctggtgt
    12421 ggaggccggg caaacggtca ccgtcacctt tggcggcaaa acctacactg caagcgtcgc
    12481 ggcgaatggt tcctggagca cctcggttcc ggcggcagat atggcggccc tgcgtaatgg
    12541 cgatgccagc gcacaggcca gcgtcagcaa tgttaacggc aacaacgcca ctactaccca
    12601 cgcttacagc gtcgatgcca gcgtgccaac ggtgaccatt aacaccattg cgggcgacga
    12661 cattcttaac gccgccgaag cgggtgctgc gctgactatc actggcagca gcacggcgga
    12721 agcggggcag acggtgaccg tcaccctcaa tggtgcaaac tatactggca ccgtacagac
    12781 ggacggcagc tggagcatca gcgtaccgcc agccgactta agcgccctga ccgccagcaa
    12841 ctacaccgtt agcgcggcgg tgagcgacaa agccggaaac ccggcctcgg ttaatcacaa
    12901 cctgacggtg gatacttccg ttccggtcgt caccatcaac acggtggcag gcgatgatgt
    12961 gatcaacgcg acggaacacg cccaggcgca gatcatcagc ggctccgcca cgggtgcggc
    13021 aaccggtagc accgtgacgg tgactatcgg cacaaatacc ttcaccaccg tgctggatgc
    13081 cagcggtaac tggagcgtcg gcgttccggc aagcgtcgtc tcggcgctgg cgaacggcac
    13141 ggtgaccatt aacgccagcg tcaccgatgc cgccggaaac agcggcagcg ccacgcatca
    13201 ggtgacggtc aatactggcc tgccgtcaat tacctttaac gccatcagcg gcgataacgt
    13261 cctgaacgcc gatgaaaaag gccagccgtt gaccattagc ggtagcagta cggggttggc
    13321 gaccggcgca caggttaccg tcacactcaa cggtcataac tacagcgcta ccaccgacgc
    13381 ggcgggcaac tggaccttaa ccgtgccggt gagcgatctg gcggctttag gccaggccaa
    13441 ctatatcgtc agcgccagcg ccaccagtgc ggcaggtaac accgccagca gccaggcgaa
    13501 tttactggtc gacagcggcc tgccgggcgt caccatcaac accgtggcag gcgacgatat
    13561 tatcaacgcc gccgaagcgg gagccgctca aaccatcagc ggggtggtga ctcgcgccgc
    13621 cgctggcgat acggtcaccg tgacgcttgg cggcaacact tatactgcac aagttcaggc
    13681 cgatttaagc tggagcgtca gcgttccggc agccgacctc caggcgctgg gcaatggtga
    13741 tttgaccatt accgcctcgg tcaccaacgc gaatggcaac accggcagcg gcacgcggga
    13801 tatcaccatt gatgccaacc tgccggggct gcgcgtggat acggtggcgg gcgatgatat
    13861 cgtcaacagc attgaacacg ggcaggcgct ggtgatcacc ggcggcagca gtggtctgaa
    13921 tgccggtgtg ccattgacga tcacgattaa cggtacggct tattccgcca cggtacaggc
    13981 ggacggaagc tggagtgtcg gcattccggc ggcaaacgtt agcgcctggc cagcgggagc
    14041 attaaccgtt gaggtagacg ggcagagcag cgccggaaat ccggtcggcg tcagccatcc
    14101 gttcactgtc gatttaacgg cggtggcaat cagcatcagc accgttgcca gcgatgacgt
    14161 aattaatgcc gccgagaaag gcaccaatct gaccctttcc ggcagtacca gcgggattga
    14221 gagcgggcaa accgtcaccg tcacttttgg cggtaaaacc tacactgcaa gcgtcgccgc
    14281 caacgggagc tggagtgtaa acgttccggc ggcagatctg gcaattctgc cagacggcgc
    14341 ggcgaacgtg caggctagtg tcagcagcgc gagcggtaac agtgcctcgg cgacccatgc
    14401 gtatagcgtt gacgccagcg caccgacgct caccattaac accattgcca gcgacgatat
    14461 tcttaacgcc acggaggcgg gaagcccgct catcatcagc ggcaccagca ccgccgaaac
    14521 cgggcagacg gtcaccgtca cccttaacgg cgcaacctac agcggtaatg tgcaggcaga
    14581 cggtagctgg agcgtcagcg ttcccccttc agccttaggc gcgctgagcg caagcaatta
    14641 caccgtcagc gccacggtca acgacaaagc gggcaacccc ggcagcgcca gccataatct
    14701 ggcggtagac accaccgcgc cggttctcac cattaacact gtggtgggcg atgacattat
    14761 caacgatgcc gaacatgcgc aggcgctggt gatctccggc accagcacgg gcggggaagc
    14821 gggcgatgtg gtgagcgttg tgctcaacgg caaaacctac accaccaccc tggatgcctc
    14881 cggtaactgg agcgttggcg ttccagcggc ggatgttgcg gcactcggta gcggtgcgca
    14941 gaccatcacc gccagcgtca gcgatcgggc aggcaacagc gacgacgcca gccgcaccgt
    15001 gaccgtcagt ctcaccgcgc cagtgattag catcaacacc atcgcgggcg atgatgtgat
    15061 caacgcgacg gaaaaaggat ctgatctggc gctttccggc atcagcgatc agccagcggg
    15121 cacggcgatc accgtcaccc tgaacggaca aaactacagc gccaccaccg actcttctgg
    15181 caactggagc gtcaccgtgc ccgcctcggc ggtcagtgcg ctgggcgaag cctcctacag
    15241 cgtgacggcg agcgtcacca atgctcaggg taacagcagc accgccagcc ataacgtgca
    15301 ggtcaatacc gcgctaccgg gcgtcaccat taatccggta acgacggacg atattattaa
    15361 cgccgcagaa gcaggcagcg cgcaaactat cagcggccag gtaaccggtg cggcggcggg
    15421 cagcaccgtc accgttgaac tgggcggtaa aacttacacc gccaccgtcc aggctgattt
    15481 aagctggaat gtgagcgtac ctgccgccga ctggcaggcg ctgggtaacg gtgagttaac
    15541 ggttaatgcc tcggtgacca acgccgttgg taacaccggt agcggcacgc gcgatatcac
    15601 catcgatgcc agtctgcctg gcctgcgggt ggataccgtc gcgggtgatg atgtggtcaa
    15661 tatcatcgaa cacagtcagg cgcaggtgat caccggtagc agctccggct ttgcggcggg
    15721 cacggcgctg accgtagtga taaataatca aacttacgcc gccacggtgc tggcgaacgg
    15781 cagctggagc gtcggcgttc ctgcgacgga tgtcagtaac tggcctgcgg gaacgctgaa
    15841 tattaccgtt agcggcgcga acagtgccgg aacgcaaacc agcattaccc atccgctgac
    15901 cgttgatctc accaccgtcg ccgtcagcat aaacagcatc accagcgatg atgtgattaa
    15961 cgccgccgaa aaaggcgcgg cgctaacgct ctccggcagc acgtccggtg tcgaagcggg
    16021 gcaaaccgtt accattactt ttggcggcaa aacctacacc accacggtgg cggctaacgg
    16081 tagctggagc accaccgttc cggctgtgga tatggcgacc ctgcgtgacg gcgatgccag
    16141 cgcccaggtg cgggtgacta acgtcaatgg caacagcgcc acggcaacgc atgaatacag
    16201 cgtcgatagc gccgcgccaa cggtgaccat caacaccatt gccagtgata acatcatcaa
    16261 cgccagcgaa gcggcggcgg gcgtgacggt atccggcacc agcacggcgg agacggggca
    16321 gacgctgacc gtcacgctta acggcactaa ctaccagacc acggtgcagg cagacggcag
    16381 ctggagttta acgctgcccg ccagcgactt aaccgcactt gccaataacg gctacaccct
    16441 gaccgcctcg gtcagcgatt tggcgggtaa ccctggcagc gccagcaaag gtgtgaccgt
    16501 cgataccacc gcgcccgtca tcagtttcaa caccgtggcg ggcgacgatg tgattaacaa
    16561 cgtcgaacac actcaggcgc agattatcag tggcaccgcc acaggcgcgg tggcgggcga
    16621 ccgcctggtg gtgaccatcg ccgggcagca gtatgtcacc agcaccgatg ccagcggcaa
    16681 ctggagcgtt ggcgtgcctg ccagcgtgat ttccggcctt gcggacggca cggtgaccat
    16741 cagcgccacc attaccgata gcgcaggcaa cagcagtacg cagacgcaca acgtgcaggt
    16801 aaacacggcg gcagtgtcgc tctcggtcag caccatcagc ggcgataata ttattaacgc
    16861 cgccgaagca ggcagtgcgc tgaccctgag cggcaccgga acgaactttg ccgcaggtac
    16921 agtagtgacc gtgttgctta acggcaaagg ctacagcgcc accattcaga gcaacgggag
    16981 ctggagcgtg aacgtgccgg cggcagacgt cgcagcactc agtgatggca ccagctacac
    17041 ggttagcgcc tccgcgcagg acagcgccgg aaacagcgcc acggcctcac gcagcgtggc
    17101 ggtggatctc accgcgccgg ttatcagcat aaacacggtt tcaacggacg accgactcaa
    17161 cgccgccgaa cagcagcagc cgttaaccct taacggctcg actagcgcgg aagtggggca
    17221 gacggtcacc gtcacctttg gcggcaaaac ctataccgcc acggttgccg ccaatggcac
    17281 ctgggcgctt aacgtgcctg cggcggatct ggccgcgctc gggcagggtg cgcagaccat
    17341 taccgccagc gtgaacgatc gcgccggtaa ccccggtcaa accacgcacg ccctgaccgt
    17401 cgataccgtt gcgccaacgg tcaccatcgc cacggtggcg ggtgacgata ttatcaacaa
    17461 cgccgagcag cttgccgggc agaccatcag cggcaccacc accgccgaag tgggccagac
    17521 ggtgaccgtt acctttaacg gtcagagctg gactgcaacg gtcggcagcg gcggaagctg
    17581 gtcggtgttt attccggcgc agcagtttgc cggattaagc gacggcagct acaccattag
    17641 cgcgacggtt agcgatcagg ccggaaaccc cggcagcgcc agccgtggcg tgacgctgaa
    17701 cggcgacgtc ccctccgtca ccatcaacac ctttgcgggc gatgatgtgg tgaatgcagc
    17761 ggagcacggc tcgtcgctgg tgatcagcgg caccaccacc gccccagtcg ggcagacgct
    17821 gaccctgacc ttaaacggca aaacctacac caccacggta cagaccggcg gtagctggag
    17881 ctacaccctc ggcagcgtcg atgtcaccgc gctggcggac ggcaatgcct acgtgattaa
    17941 cgcctcggtg agcaacgcta ttggcaacac cggcagcagc aatcacacca ttactgtcga
    18001 tctcagcgcc ccggcgatgg gtattaatat cgattccctg caagccgaca ctggccttag
    18061 ctccagcgac tttatcacca gcgtcagtcc ggtggtggtc aacggctcgc tcaccgccgc
    18121 gcttgccagt aacgagacgg cacaaatcag tatcgacggc ggcgtaacct ggaccacgct
    18181 caccgttacc ggcacgacct ggcgctataa cgacagccgc acgctgaccg atggcagtta
    18241 tctctatcag gtgcgggtga ttgacgcggc gggcaacgtc ggtgccactg acagccagaa
    18301 tgtggtgatc gacaccatcg cgccagatcc cgcggtgaaa accatcgcca tcaacgcgat
    18361 caccaccgat actggattga ttactaacga ttttgtcacc agcgacacga cgcttgcggt
    18421 aagcggcacg ctgggggcgg cgctttcttc cggtgagttc gcgcaaatca gcattgatgg
    18481 cggcaccacc tggcagaatc tttcggtaag cggtttgacc tggacatatc ttgacgggcg
    18541 gacgctgagc gatggcaact acaactatca ggtgcgggtt atcgacaccg ccggaaatat
    18601 cggcgcgacc gccagccaga tagtcactgt ggacaccacc gcgccgctgg cgagcaaaac
    18661 catcgccatt gcgggcatca gcgatgacac tgggttaagt agcagcgatt ttgttactcg
    18721 cgacaccacg ctgaccgtgc gcggcacgct cggcgcggcg cttgccgccg atgaacgggc
    18781 gcaaatcagc ctcgacggcg gtgtcacctg gaccacgtta accgttatcg gcaccagctg
    18841 gagctatgcc gatggtcgca cactgaccga cggcacctgg aactacacgg tgcgggtggt
    18901 ggatctggcg gggaacgttg ggcagaccgc aacgcaaaat gtggtggtcg acaccaccag
    18961 cccggaagcg gcgaaaagta tcaccattac cggtatcagc gatgacaccg gagccagcag
    19021 cagcgatttt attaccagcg acacctcgct taccgtgcgc ggcgtattgg gggcggcgct
    19081 cggcgctaac gagttcgcgc aaatcagtac cgacaacggc gcaacctggg tgaacgtaac
    19141 gctggcggca gatggcctga actggagtta cgttgacgga cgcacgctaa cgaatggcac
    19201 gacgacctgg caggtgcggg tggtcgatct ggcgggcaac gttggcgcaa ccagcagcca
    19261 gtcggcgcag atcgacaccg ttaacccggc gcaggtgttg accatcgcca gcatcagcac
    19321 cgacacgggg agttcggcaa ctgactttat caccagtgac accacgctca ccctaaccgg
    19381 ttcgctgggg gcggggcttg ccagcggcga agtggcgcag attagcctcg atggcggcgc
    19441 gacctggatc acactcacca ccaacggcac gcagtggacc tacactgaca gccgcacgct
    19501 gaccgacggc agctatgttt atcaggtgcg ggtgctggat ctggcgggga acaccggccc
    19561 ggtggtgtcg aaaacggtgg tggtcgatac gattaaccct accgccacgc cgaccattgt
    19621 gtcgtatacc gatgatgtcg ggcagcggca ggggacatta agcagttcgc aggccaccga
    19681 cgacactacg ccgttgctga acggcgtgct ctccggcccg cttgccagcg gtgaagtggt
    19741 ttacctctac cgtaacggcc tgctgttagg cgcggtaacg atggtcggcg cactgaactg
    19801 gacctacagc gacagcgggc tggtgagcgg tgcctacacc tacagtgcgc gagtggtgga
    19861 tctggcgggg aatatcaccg cctccagtga ttttgtcctg acggtcgata cctctattcc
    19921 gaccacgctg gcgcaaatta ccagccagac cacgcgcgat accacgccga ttatcagcgg
    19981 ggtaatcacg gcggcactcg ccagtgggca gtatgttgaa gtggtgatca acggcaaaac
    20041 ctacacctct gaaccgggtg gcgcggtagt ggtcgatccg gcgcacaaca cctggtatgt
    20101 acagttgccg gataccgacg cgctggcggc ttctgcgacc gcctataacg tcaccgcgca
    20161 ggtaaaaagc tcggcgggca acggcaataa cgccaatatt agcaacggca cggtgacggt
    20221 taacgcggcg attgattaca caccgacctg gaccaccgcc agcaaaacca ccgcctgggg
    20281 attgacctac ggccttgact cgcacgggat gtggacggtg ctggcaaacc agcaggtaat
    20341 gcaatcgact gacccactca cctggtcgaa gaccgcgctg acgctgtatc agagcggcaa
    20401 caactacgcc accagctcca tcgccgatta cgaccgtaac ggcacgggcg atctgtttat
    20461 cacccgtgat gactacggta caggctatat caacggcttt accaataacg gcgatggcac
    20521 cttttccagc gctattcagg tcaccgtcgg caccctgacg tggtacggct cgattgtggc
    20581 atttgataaa gagggcgacg gctatctcga cttctggatt ggcgacgcgg gcgggccgga
    20641 ctccaacacc ttcttgtgga acaacgcagg tacgctggta ggtaactcca ccacgtcgaa
    20701 cagcggcggt agcgccacgg tgggcggggc ggtgacaggg tatctttcgc tcaacgaagg
    20761 ttctggtgtc gatctgaaca atgacggcag gatcgacctg gttcagcaca cctacaacct
    20821 gaacaactat tacacgctat cttcgctcat caaccaaggg aatgggacgt ttgtctgggg
    20881 gcagaacacc accaatacct tcctgagcgg ggcgggcagt ggcgctatga gcagcagcgt
    20941 ttccatgacc tgggccgatt tcgatggtga cggcgatatg gatctcttcc tgcctgccag
    21001 ccagggaaga gctaactacg gctcgctgtt attcaacacc aacggcgtac tgggtagccc
    21061 ggtggcggtg ggcgcaacgg caaccaccta tgccagccag tttagcgtgg cggtggactg
    21121 gaaccatgac ggcctgatgg atatcgcccg tatcgcccag accgggcagt cgtatcttta
    21181 taccaacgtc agcaatgcca gcaactggac acaatcggcc ctcggcggca gccagagcgg
    21241 taccaccagc ggcgtggcgg caatggacta cgactgggac ggcgcggtgg atgtgctggt
    21301 gaccaaacag tcgggcagcg tctatcttat tcgcaacacc aacacggtga gctacggcac
    21361 ctcgctgcac ctgcgcatca ccgatcctaa cggcattaac gtctattacg gcaataccgt
    21421 aaaactgtac aactcggcgg gagtgctggt cgccacgcaa atcatcaacc cgcagtcggg
    21481 gatgggggtt aacgacacct cggcgctggt caacttctac gggctgaatg ccggagaaac
    21541 ctacaacgcg gtgctgatca aatccactgg caccaccgcc agcaatatcg accagacggt
    21601 caacaccagc tggggcggct tacaggccac cgatgccact cacgcttacg acctcagcgc
    21661 cgaagcaggc accgcgagca acaatggcaa gttcgtcggc accggctata acgacacctt
    21721 ctttgccacc gcaggcaccg acacttacga cggttccggc ggctgggtgt acagctccgg
    21781 caccggaacc tggcttgcga acggcggcat ggacgtggtt gatttccgcc tctcgacggt
    21841 gggcgtgacc gcgaacttaa gcagcaccgc cgcgcaggcc accggcttta acacctcgac
    21901 atttaccaat atcgaaggga tatccggctc gaattttaac gacactctga ccggcagcag
    21961 cggcgataac caactggaag ggcgcggcgg taacgacacg ctcaacatcg gcaacggcgg
    22021 ccacgacacc ttgctctata aactgctcaa cgcaagcgac gcgaccggcg gcaacggctc
    22081 ggatgtggtg aatggcttta cggtagggac atgggaaggc accgccgaca ccgatcgcat
    22141 tgatatccgc gagttgttac agggcagcgg ctacaccggc aacggcaaag ccagctacgt
    22201 caacggcgtg gcaacgctgg atgcgcaggc cggaaacatc ggtgattttg tcaaagtcac
    22261 ccagagcggc agcgacacca tcgtgcagat cgaccgcgac ggcacgggcg gcacttttgc
    22321 ggcaactaac gtggtcacgc tgacgggcgt gcataccgac ctcgccactt tgctggcgaa
    22381 tcatcagttg atggtggtgt agagaatgaa aaaaaatgcc cagtccgtgg aagcctggct
    22441 ggaggcgatg atcgccgtgg cgcgctatta ccgccttgat ttttctcagg agaatgtgcg
    22501 cgcgaccgtc aactgggagc gcgacagtaa gcgggaggag ttactcaccg acatggcgcg
    22561 gcagttgggg atggggcttc ggctggtgga gttctccacc gactcgctca acccctggcg
    22621 gctgccgttg atcgccgttt ttgataatca acaaattggc gtgatcaccc gccgcgataa
    22681 tcacgacaat atcagcgttc agttcagcgg tgacgaaggg ctggaaacca cgcttaacgt
    22741 tacggatatt gaagataaca tcgtcgaact ggcgctgttg cgcccgctca gcgctattcc
    22801 tgacgcgcgg gtagacgact atattcgccc gtatcaggcg aactggttct ggaatctctc
    22861 gctaaaagac tggcggcgct atggcgatat tatgctggcg tcactggtgg cgaacgtgct
    22921 ggcgcttgcg gcgatgattt tctcgatgca ggtttacgac cgggtggttc cggcgcagtc
    22981 ctacccgacg ctgtgggtgc tgttcgccgg ggtgatgatg gcgatcctgt ttgaattctg
    23041 tatgcgcatg gtgcgcacgc acctttccga tgtgattggt aagcgggcag atctgcgcat
    23101 ttcggatcgc gtttttggtc acgcattacg gctgaaaaat aacgtgcgat cgaaatccac
    23161 cggatcgttt atctcgcaga tccgcgaact ggaatcggtg cgggagctga tcacctccac
    23221 cactattggt gcagtggcgg atctgccgtt cttcctgctg tttgtcttta ttttgtggat
    23281 gattggcggc tggctggtgc tggtggtttt gctggcgctg ccgttattgg tgatccccgg
    23341 cctgctggta caacgcccgc tggcgcggct ggcgaacgaa gggatgcgcg agtcggcggt
    23401 gcgtaacgct acgctggtgg aagccgtgca gtcgattgaa gacattaaac tgctgcgcgc
    23461 tgaacagcgt tttcagaatc agtggaatca cactaacgac gtggcctcgt ccatcagcat
    23521 gaagcaacgt tttctcaccg ggctactgct cacctggacg caggaagtgc aatccatcgt
    23581 ctacgtcgtg gtgctgttgg tgggctgctt tatggtaatg aacggtgaca tgactaccgg
    23641 cgcgctggtc gggacgacga tcctggcttc gcgcaccatc gcgccgctgt cgcagatatc
    23701 cggtgtgctc tcgcgctggc agcaggcgaa agtggcgcgc aacggacttg atgaactgat
    23761 gaagcgcccg gtggatcagc cggaacacgg caaactggtg cataaagcgg tgctacacgg
    23821 caattatcag ttcagcaacg ctatctttta ttacgatgaa gaagagaaaa ttgccgatgt
    23881 ggcgattggc aaactcaaca tccaggccgg agagaaaatc gccattctcg ggcgcaacgg
    23941 cgcgggtaaa tcaacgctac tgcaaatgct cgccggaatg cgtatcgccc agcaggggca
    24001 ggtgctactg gataacatca gtatcgggca gcttgatccg gcagatctgc gccgcgacat
    24061 ggggctactg agccagaccg gacgactgtt tttcggctcg ctgcgggaaa acctgacgat
    24121 ggggatgccg gaggccagcg atgaagatat cgaacgcgca ttaaccctca gcggggcgct
    24181 gccgtttgtg cagaagcaga aaaacggcct caactacatg attcaggaag ggggatttgg
    24241 tctttctggc ggtcagcggc aaacgctgct gctggcgcgg cttctcatct ctcaacccaa
    24301 tatcgtgctg ctcgatgaac ccagcgcctc gctggatgaa atggcagaag cgtatttaat
    24361 cgaacaactg aaacagtgga ttggccatcg cacgctgatt atcgccactc accgcacggc
    24421 gatgctgcaa ctggtggacc gcattatcgt gatggatcag gggcggattg tgatggatgg
    24481 cgcgaaagag gccattttgc gtgaacaggg cgaacccacg acgcgacggg tggtgttgca
    24541 ggagaagaat aaggggtctg ccgcatgaat ggacaactgc aatacaaccg tggcctgact
    24601 gaaccccgcc tgccgcgttc ggcgctggcg gtacgcgtta ccgctgtgat gctgctctgt
    24661 tttctcggct gggcgtggta cttccagctt gatgaagtca ccaccggcag cggcacggta
    24721 gaaccttccg ggcgcgaaca ggtggtgcag tcgctggaag gcggaattct gtatcatctc
    24781 gacgtcaagg ttggcgatat tgttgaacag ggccagccgc tggcgcagct taaccgcacc
    24841 aaaacggaat ccgacgtcca ggaagcaatg tcgcggctgt acgcggcgct ggcaacctcg
    24901 gcgcggctgc gggcggaagt gagtaataaa ccgctggtct tccctgacga actgaataaa
    24961 ttccccgaac ttatcgagtc ggaaacggcg ttgtacaaca ctcgccgcga tgggctgaac
    25021 aaagccacca ccgggctgac gcaggggatt agcctggtca accgcgaact ggcgatgacc
    25081 cagccgctgg taaaacaggg ggcggcgagc agcgttgaag tgctgcgcct gcaacgccag
    25141 gccaatgaac tggaaaacaa actcagcgac gtgcgcacgc aatactacgt acaggcgcgg
    25201 gaagagttag cgaaagccaa tgcggaagta gaaacccagc gttcggtgat ccgcgggcgg
    25261 gaagattctc tcacccggct caactttacc gcgccggtac gtggcattgt tcaggacatt
    25321 gacgtcacca cggttggcgg cgttattgcc cccggtggta aactgatgac catcgtgccg
    25381 ctggatgagc aactgctgat cgaggcaaaa atctccccgc gcgatgtggc gtttatccat
    25441 ccggggcaaa aatcgctggt gaaaataacc gcctacgatt actccatcta cggcggcctg
    25501 ccgggagaag tggcggtgat atcaccagac accgtgcagg atgaagtacg gcgcgatgtt
    25561 tattactatc gggtgtacat tcgcactttc agcaaccatc tggaaaacaa aagcaaacag
    25621 caatttccga tatttcccgg tatggtggcg acggtcgata tccgcaccgg caaaaaaagc
    25681 gtgctggatt atctgttgaa accctttaac aaagcacagg aggcgttgcg cgaacgatga
    25741 acattagcga tgtagcaaaa attaccggcc tgaccagcaa agcaatccgc ttctatgaag
    25801 agaaggggct ggtgacgccg ccgatgcgta gcgaaaatgg ttatcgaacc tacacgcagc
    25861 agcatctcaa cgaactgacc ttactgcgcc aggcgcgcca ggtgggcttt aacctggaag
    25921 agagcggcga actggtgaat ctgtttaacg acccgcagcg tcacagcgcc gatgtaaaac
    25981 ggcgcaccct ggagaaagtg gcggagatcg aacgacacat tgaggagctg caatccatgc
    26041 gcaaccagct gttggcgctg gcgaatgcct gccctggcga tgacagcgcc gactgcccga
    26101 ttatcgaaaa tctctccggc tgctgtcatc atcgggcggg gtgaaaatca gttaatcccc
    26161 gtttgcaccg aaagaatatg ctgcaaactt tctaccataa tgcgccccga ctgaaataag
    26221 ccttcctgga tttcgttgtt aacattcaca gcgtgaacca gggaggctaa taatactact
    26281 tgcaagaagg caaaaataaa cgcaccgata atcataatat gcgtcagggg tattttcctt
    26341 tgctccttca ttttaaataa cccacaaatt gaccttcctt cattaaggcg acttttcctg
    26401 tgtgatgata aacaatgacg ttatacagac ctgactgata aacagtggca tctttattgt
    26461 cttcatcaat tgcacataat gaatcattga gataagcaaa gtttttactg ctcattctaa
    26521 aggtcattcc aaatccttta taaatggctt tctttgccgc ctgggcatgt tgtgcggctt
    26581 ctgcattttt aaatgcctgc gcctcggatg cacttaaaat agccgtctga cgctgttgtt
    26641 ctgcgagata acaggtgtca tggctgacgc cctgcgcctg gcaatctgcg atgtgggaag
    26701 tgggtgaact acagccagcc agtaatataa ccgccagcag ggaaaacgat ttaatggagc
    26761 tattcatcat tgttaacctt aaataatgta cggaaaattg cgaataagaa attgctgtta
    26821 aaattagaac gaatatccca cgccgacacc cgcaccgaaa tcagattgtg tatccgcaga
    26881 aatacctgct ttaagtaccg tatgcgttcc ggcattaaaa gatgttccta cagacaccgc
    26941 agattcaccg ttgaaggttc ccgctcccgc tgacaccatc atcaagtcac cggttttcac
    27001 ctgtggttgt gaggcgatag cgatggctga tgcagttccc gcattggctt ctttacgatt
    27061 gtcctcaact tcgtttttca gagaatcgaa atggttattg agcgtgttaa tttgttggct
    27121 gttatctgcg acccgttttt gcgtgttggt taactgttgc tgcgtttgtg cggaatcttg
    27181 tctcagactt tcaatttgct catgtgcctg gacagacttc tgcgccagac tttgcatttg
    27241 tttatcgtat tgagcccttt ctttttgctg ttgtgctgcc acatcggttg tcagtgcatt
    27301 cattttctgc ccttgttccg tggtagtgcg ctgattagcc tcattttgag ccgccagtct
    27361 ggcattaatc atctcctgat aatgcgcagt attttgcgta tctccagtcg cggcgagcgt
    27421 tttttgtgtg gctacgatct gcgcctgttg ctctgtaatt tgcgtttgct gggcatcagt
    27481 taccgcagac tgtcgggcgt tgatcatgct ctgataacgg gcggttgtct gtacatcacc
    27541 tgtagcggca acggtttttt gtcctgcgtc aggatctgtc tggacagtgg gagtctgagt
    27601 ggtatcaggt gtttcagaag ggatgtagtg atgttcagtc aaataggtat acgcagcggg
    27661 gctgttgtct gtgcgttcta aaaagcgggt aatcttctgc tcaatctccg attgcgacat
    27721 gccagagtta atggctgaaa gctctgttgc cgccgcttca atggtatcac cagctaaaac
    27781 aacagggctg gatgttgctg aaattatgag tgccagtaaa gctacgttta cagttttcat
    27841 tcattttctc ttgttcaaaa taagtcgcat taatgtttct ataagaaaca aataatattg
    27901 ttttaagaaa taaactgcgt tgcaatttga tcgaaatcta cgccgccatt tgtgtgatgt
    27961 aaagcgcatt tttgtctggt gtaagcgtaa acaccaccaa taagaaacaa aaaatgtttc
    28021 ttatataaat aaaatcgaaa atcattttgt gctctttcaa tgtaaaaaag agtaaagact
    28081 tgttttgata agcgaaataa attctgtttc tggactaaac gtgaagcggt tcctgatgtg
    28141 tgctaaaatg cgtttctttg ataaatctca atttttaaga ggtttacgcc aggcaggaaa
    28201 atttaaatga atgaatttga gaagattttt aatgaaatga atctggaccg ggcattgcta
    28261 ccgatccttt ttcgttcgaa ccggtcaaca gtgtggaaat atttgagtgg tgatagtacg
    28321 gctcctgcct cagctatgtc actgattatg ctgttgcagt taatccagaa acgaaacccc
    28381 gatctgttag ccgaatggtt aacgctttct gatttcacca ttccgccaga agtttacctg
    28441 gaccagcctg attactggaa agggtgggtg tatacgcaac acaaggtcaa taaaaacgtc
    28501 ctggaatatc taaaaaaaca ttatccggat gaagaccaaa aaaacatgaa taaaggtagt
    28561 gacgagtaat ttgccaatgc cagttaatat agcggaggcg gcctacgatt cgggcgcaga
    28621 cttccaggca tgataagacg cgcccgcgcc gcatcaggca tcagcgcata accgccggat
    28681 gcggcgtaaa cgccttatcc ggcctacaat tcgggcatat tttgtaggca tgaaaagacg
    28741 catcagcgtc gcatcaggcg atgaccgcac ggataatcag cgttatccct tctatcgcaa
    28801 tcacctcaac atgcgtacct gcactgagat cctcgctggc gctgacaggc catgaactgt
    28861 cgccgacgcg catatgacct cgcccattga ccagcggtga ttccagcaca aaacgtcgac
    28921 caatcagctg ctgcccccgc tggtttaaat gactatcgct gtgcttttgt tcgcgcaccc
    28981 gccgcgacaa ccatttccac cacagccagg cggcgagcag cgtcaggatg gcaaacatta
    29041 ctccttgcca ctcccaaccc agcggcacca gccagactac caggccggta atcaccgcgg
    29101 ccacgccgct ccacaacaaa taaccatttc cgcccagcat ctcggctgcc agcaacaacc
    29161 cgccgagact gagccagaaa atatgtggat gaacgaccat taactccatc atggctgagt
    29221 ccgtttgttg gcgctgtctt tcaccagctc ggcaatcccg gcaatcgacc ccatcaggct
    29281 gctggcctct aatggcatca tcactacttt gctgttactg gaggaaccga tctgctgtaa
    29341 cgcttcggtg tatttctgcg ctacgaagta gttcaccgcc tgaatatcac cggaggcgat
    29401 agcttcagac accattttgg tggcgcgggc ttctgcttcg gcagaacgtt cacgcgcttc
    29461 agcctgtaaa aacgccgact gacgttcgcc ttccgctttc aggatttgcg actgtttttc
    29521 accttcagct ttgaggattt ccgcctgacg gatcccttcc gcttcgagga tgtaagcgcg
    29581 tttggtacgt tctgctttca tctgcgcgtt cattgaagag ataagctctg ccggtgggcg
    29641 cacgtcgcga atttcaatac gggtgacttt aatcccccac gggttagtgg cttcatcgac
    29701 aatacgcagc aggcgtgagt tgatgctgtc gcgctgagag agcatttcgt caagttccat
    29761 tgaacccagc acggtacgga tgttagtcat ggtcaggttg atgatcgcca gctccagatt
    29821 gctgacttca taggcagcgc gtggcgcgtc aatcacctga ataaaacaga ctgcgtcgat
    29881 ggtaacgttg gcgttatctt tcgagataac ttcctgggaa gggatatcga gcacttgctc
    29941 catcatattg atcttgcgac caatgcgatc cataaacggc accaccagac ttagcccagg
    30001 ctgtaacgtt ttggtatagc gaccaaagcg ttctactgtc cactgatagc cctgcggcac
    30061 gattttgacg cccgcgccga caatgaccag cgcgacaaaa atgagaatcg ggataaagat
    30121 aagcatcgga aaaacctcct gttgtaccgt ccataatcag caaaattgct gcttgattaa
    30181 acaaattata cctgattact gaaagagagt tcccccttat tcctgggaag gataaactgt
    30241 ttttagtaaa aatcagaaaa agggaacagc gatgcaggaa aatagtcctt tgcttcagct
    30301 acaaaacgta ggatatctgg cgggtgatgc gaagattctt aataacatca atttttcgct
    30361 gcgtgctggc gaatttaagt taattaccgg tccttctggt tgtggcaaaa gtacgctgct
    30421 aaaaatagtt gcttcattga tcagcccaac cagcggaacg ttactgtttg aaggtgagga
    30481 tgtcagcaca ctaaagccag aaatctaccg ccagcaagtc tcttactgtg cccagacacc
    30541 gacgctgttt ggcgatacgg tatacgataa tctgatcttt ccctggcaga tccgtaacca
    30601 gcagcctgac ccggccattt ttctcgattt tctcgaacgc ttcgctttac cggacagcat
    30661 tttgacgaag aatatcgccg agctatctgg gggtgaaaaa caacgcatct cattaattcg
    30721 taacctgcaa tttatgccga aggttttatt gctggatgaa atcaccagtg cgctggatga
    30781 aagtaataaa cataacgtta atgagatgat ccaccggtat gtgcgcgagc aaaatattgc
    30841 cgtgctgtgg gtgacacacg ataaagacga aattaatcat gcggataagg tgattacact
    30901 gcaaccgcat gccggagaaa tgcaggaagc acgctatgaa ctcgcataat attactaacg
    30961 aatcattggc actggcatta atgctggtgg tggtggcaat cttaattagc cataaagaaa
    31021 aactggcgct ggagaaagat attctctgga gcgtcgggcg agcgataatt cagctgatta
    31081 ttgtcggcta tgtgctgaag tatattttca gcgtggatga tgccagcctg acattattga
    31141 tggtgttatt tatctgcttt aatgcggcgt ggaacgcgca aaaacgcagt aaatatattg
    31201 ctaaagcttt tatctcatcg tttattgcca ttacggtcgg ggcgggaatt accctggcgg
    31261 tgctgattct ctctgggtcg attgaattta tcccgatgca ggtgatccct atcgccggga
    31321 tgatagccgg taacgccatg gtagcggtgg ggttgtgtta caacaattta gggcaacggg
    31381 tgattagcga acagcaacag attcaggaga aactgagtct tggcgcgacg ccgaaacagg
    31441 cttcagcgat attgattcgc gacagtattc gcgcggcttt aattccgacg gtcgattcag
    31501 caaaaacggt tggcttagtg agtttaccag gaatgatgtc cgggctgata tttgccggga
    31561 ttgatccggt gaaggcgatt aaatatcaga ttatggtgac ctttatgctg ctctcaactg
    31621 ccagcctgtc taccattatt gcctgctatt taacctatcg taaattttat aattcgcgcc
    31681 accagttggt ggtgacgcaa ttgaagaaga aatgatgcaa tgccggatgc gcagcatcgc
    31741 atccggcgtt gtggttcatg tgccgatcaa cggaaactca atacaacaac gcatacagct
    31801 ggcggcgata cttcgacgcc agtgcatcac ctgtacccag cgcagcgagg atctcctgga
    31861 acgttttacg cgtctgaccg tctgcggcag tgagatcttt acgcagatgc ccaaacagta
    31921 actccagcgc ctcttcattg cgcccaacct gatgcagttg cagcgccagt tgcgtcgcca
    31981 gtgcggcatc ttctggattc tctgccacct gctgttgcag ctgttgaatt tccggcgtat
    32041 cagccgcctg cttcagcagt tcgatttgcg ccaccagccc ctgatagcgg gtgtcctgat
    32101 cctgtaacgg aatggttttc agcacggctt cagcatcttc agaacggttc agcgcaatca
    32161 gcgtttctgc cagcagcagg ccgatctccc cgttctgatt cgacaactgc caggcgtctt
    32221 tcagcaatgg cagggcgtcg gtgtaattgc cttcttgcat cagttgcatc gcctgctgtg
    32281 ctttcagttc ttcttcgcgc ggcagcactt tatccagcag ggcgcggatc gcctcttccg
    32341 gttgcggccc ctggaagccg tctaccggtt gcccgttctg gaacagatac acggtcggaa
    32401 tcgcgcgcag accaaactgg gcggcaatca tctgctccgc gtcgcagtcc agcttcgcca
    32461 gaataaattg cccgttgtac tgcgccgcga ggctttccag aattggggtt aactgcaaac
    32521 agtgctggct acgttcagac caaaaataga acagcaccgg agtggtcatc gactgttcaa
    32581 gaacctgttg caggttagat tcgttaatgt tgacaatatt ttctacggac atggagtcgc
    32641 tctctgttgt cgatattttc tttgacatgg gggcttaagc gcgcgcttca acttacccct
    32701 gcaatatttt gtccatcacg cgccccggca gcaggcgctt aagcaccatt actgcccagg
    32761 tcaccagcgt taccggatag cgcatcttcg gtttctcgct aataaaagca tggcgcactt
    32821 tgtcgaccac cgcttccggc cccaacgtaa agcgggcggc gatgccggga ttttcgacag
    32881 gtttatcact ttgcgtctgg ttgacgttgt cggtgaagcg agtacgaatg ggaccgggtt
    32941 cgatcaggct aactttaatt ccgctgtggc gtagctccat gcgcagcgca tccgaccacg
    33001 cctccagcgc atatttactg gctgcgtaag cgccgcgacc cggcgtggag attaatccca
    33061 tcaccgatga ggtcatcaca atacgccctt caccgtgcgg taacatcgcg ggtaacaggc
    33121 gcatggtgag ctggtgtgcg ccgaaaaagt tggcagaaaa ctgctgttcc atctgcgcac
    33181 ggctgatggt ggaaaggggg ccatacatgc cgaatccggc attgttaaag atcccataca
    33241 gacaattatc ggtcagggcg atcacttcgt cggctgcgcg atcaacactt tctggtgaat
    33301 ccagatcgat caacacgccg gtaaatccca tgctgttcat gcgctcaaca tcatccggtt
    33361 tccggcaacc tgccagcaca tgaaaaccct ggcgttttaa ttcgagcgcg ctttccaggc
    33421 caattccact ggaacatccg gtaattaaga ccgatttttg cataacttta cctgtcagga
    33481 tctccgttgc tttatgagtc atgatttact aaaggctgca actgcttcgc catccagtcg
    33541 gcaataaacg gctgggcgtc gcggttggga tgaataccgt catcctgcat ccattgtggc
    33601 ttgaggtaga cctcttccat aaaaaagggc agcagcggaa catcaaactc tttggcgagt
    33661 ttggggtaaa tggcgctaaa ggcttcatta taacggcgac catagtttgc aggcagacgt
    33721 atttgcatta acaatggttc agcgttggcg gctttgacat cctgcaaaat ctggcgcagc
    33781 gtttgctcgg tttgttgtgg ctgaaaacca cgcaaaccgt cattgccgcc cagttcaacc
    33841 agcacccagc gcggctgatg ctgtttcagc agagccggaa ggcgcgccag cccttgttgc
    33901 gaggtgtcgc cgctgatgct ggcattaact accgacgttt tactctgcca cttatcattc
    33961 aacaaggcag gccaggccgc gctggcagac attcgatacc cggcgctcag gctatcaccc
    34021 agaatcaata acgtgtccgc tgcggcggca cggaaggtta acaggaccag gaacaggaag
    34081 ggcaaatgcc agcggaaaac attgttgaag ttcatcatct taagaagtcc gtcggtcagg
    34141 gggagcatga actctccatc ctcaccggag ttgagctggt tgtcaaacgt ggcgagacca
    34201 tcgcactggt gggtgagtcg ggatcgggta agtcaacctt gctggcaatc ctcgccgggc
    34261 tggatgacgg cagcagtggc gaagtgagtc tggtgggaca accgctacat aatatggacg
    34321 aagaagcgcg ggcaaagttg cgcgcgaagc acgtaggttt tgtttttcag tcatttatgt
    34381 taattcctac ccttaacgcg ctggaaaacg tcgagcttcc ggcgctgctg cgcggtgaga
    34441 gtagcgcgga aagtcgtaac ggggcgaaag cgttgctcga acagttaggg ctgggtaaac
    34501 gtcttgatca tcttccggca cagctttccg gcggcgaaca gcaacgagtg gcgctggcgc
    34561 gagcctttaa cggtcgacct gatgtgctgt ttgccgacga acccaccggc aaccttgacc
    34621 gccagacggg cgataaaatt gccgacctgc tgttttccct caaccgtgag catggcacca
    34681 cgttgattat ggtgacccac gacctgcaac tggcggcacg ctgcgaccgc tgcttacggc
    34741 tggtgaacgg gcagttgcag gaggaagcat gattgcacgt tggttctggc gcgaatggcg
    34801 ctcgccgtcg ctattaattg tctggctggc gctaagcctg gcggtggcct gcgtgctggc
    34861 gctgggcaat atcagcgatc gcatggagaa gggcttaagc cagcaaagcc gggagtttat
    34921 ggcgggcgat cgggcgttgc gcagttcgcg cgaagtgccg caagcgtggc tggaggaagc
    34981 gcaaaagcgc ggcctgaaag tcggcaagca gctgactttc gccaccatga catttgcagg
    35041 cgacacaccg cagctggcga acgtcaaagc ggtggatgat atctatccga tgtatggcga
    35101 tctgcaaacc aatccccctg gcctgaaacc gcaggcgggc agcgtattgc tggccccacg
    35161 cctgatggca ctgcttaacc tgaaaacggg cgacaccatc gacgtgggtg atgccacctt
    35221 gcggattgcc ggagaagtga ttcaggaacc ggattccggt tttaacccct tccagatggc
    35281 tccgcgtctg atgatgaatc tggcggatgt cgataaaacc ggagccgtgc agccgggaag
    35341 tcgggtcacc tggcgctata aattcggcgg caacgagaac cagctcgacg gctatgagaa
    35401 atggttgtta cctcagctta aacccgaaca acgctggtac ggtctggaac aggacgaagg
    35461 cgcgctgggg cgctcgatgg aacgctcgca acagttcctg ctgctttcgg cgcttctgac
    35521 cttgctgctg gcagtggcag cggtggcggt ggcgatgaat cattactgtc gcagtcgcta
    35581 cgatcttgtg gcgatcctca aaacgctggg ggcagggcga gcgcaactgc gtaagctaat
    35641 cgtcggtcag tggttgatgg tgctggcgct ttcagccgtt accggcgggg cgataggcct
    35701 gttgttcgaa aacgtgttga tggtgctgct caagcccgtt ctgcctgccg cactaccgcc
    35761 agccagcctc tggccgtggc tatgggcgct gggcaccatg acggttatct cgctgctggt
    35821 ggggctacga ccgtaccgct tgttgctggc aacgcagcct ttacgcgtat tacgtaatga
    35881 tgtggtagcg aacgtctggc cgctcaagtt ttatctgccg attgtcagtg tggtggttgt
    35941 gctgctgctc gccggattaa tgggtggcag catgctgctt tgggcggtgc tggcgggcgc
    36001 ggtagtactg gctttgctgt gcggtgtgct gggctggatg ctgctgaatg tacttcgccg
    36061 catgacgctg aaatcgctat ctctgcgcct ggcggttagc cgcctgttac gtcagccgtg
    36121 gtcaacgtta agccagcttt cggcattttc gctctccttt atgctgttgg cactgctgct
    36181 ggtgctgcgt ggcgatctgc tcgaccgctg gcagcagcag ctacctccag aaagcccgaa
    36241 ctacttttta attaacatcg ccacagaaca ggtagcaccg ctaaaagcgt tcctcgcgga
    36301 acatcagata gtcccggaat cgttttatcc ggtggtgcgg gcgcggctga cggcgattaa
    36361 cgataagccg acagaaggta atgaagatga ggcgcttaac cgcgaactca atcttacctg
    36421 gcaaaatacg cggcccgatc ataatccgat tatcgccggt aactggccgc caaaagccga
    36481 tgaagtgtcg atggaagagg ggctggcgaa acgcttaaac gttggcctcg gcgatacagt
    36541 cacctttatg ggcgataccc aggagttccg cgctaaagtg accagcctgc gcaaagtgga
    36601 ctgggaaagt ctgcggccta atttctattt tattttcccg gaaggggcat tagacgggca
    36661 accgcagagc tggcttacca gtttccgctg ggagaatggc aatggcatgt tgacgcaact
    36721 caaccgccag ttcccgacca ttagcctgtt agacattggc gcgattttaa aacaggtcgg
    36781 tcaggtgctg gagcaggtaa gtcgggcgct ggaggtgatg gtggtgctgg tcactgcctg
    36841 cggtatgttg ctgttgctgg cacaggtgca ggtgggaatg cgtcagcgtc atcaggagct
    36901 ggtggtgtgg cgcacactcg gtgcggggaa aaaactgctg cacaccacgt tgtggtgtga
    36961 attcgccatg ctcgggtttg tttctggcct ggtggccgca attggtgcgg aaacggcgct
    37021 ggcagtgttg caggcgaaag tgtttgattt cccgtgggag ccagactggc gattgtggat
    37081 tgttctgccg tgcagcggtg cgctgctgct gtcgcttttc ggcggctggc tgggtgcgcg
    37141 actggttaag ggtaaggcgc tgttcaggca gtttgcggga tgatgacagg tgttagattg
    37201 cctgatgcgc tacgcttatc aggcctacga agagcgtgca atatattgat tttgcaagat
    37261 tgtgtaggcc ggataaggcg tttacgccgc atctggcatg aacagtatgc attttggcaa
    37321 taaatatgaa accaactgtc atgtcggttt ttttaaaggc cgaaggtggc aaaccttcgg
    37381 ccttagctac gtttcctcag ttattcaata aactgacgaa tcagttccag cagcgcgatt
    37441 agcgccttaa gaacgatgag aactttatcg agaaaagttt tcatccgttg ctcccttaag
    37501 ggagaaagac aatcacagct tacccttacg ttgcctgtaa ctatgatttt cttcatgtgc
    37561 tagatgagga ctgcctctct ccaaattaga gcaccagaat cagcaccacc tgtatcctga
    37621 ccaggactta aaagtacttt tccgcactgc ggtaatccct tgaggcagta tcactactag
    37681 cgtcgggatt gtatcgccaa catagctgtt tgaatatgta ggcatcctga cttataattc
    37741 acttgctaga tgaggactgt ttcatttctg cccggaacca ccaagtcttg ggcggaaaaa
    37801 ataaaaaaac cgactgtcat gtcggttttt ttatatctgt attctggtgc aaccattctg
    37861 ttctcacgac aatcgccgtt tcagaatacc gcccatcact gcgccttaac ccattccgcc
    37921 acttccgccc actcaccgcg aaagacaact ttttcagctt ttttctcaag ctgatagcga
    37981 tacatcgggt cgtaatattc ttcaagtaac ggcaccagcc aggccagatg accgtcggtg
    38041 ctgccggtgg tgagttgcgt tgtcagcgca gcatccagcc ttgcagccag ttcgttatat
    38101 cgctgtaacc ccagccgacg cttaatcgcc gaaagtccgt gatgcaggta ttcgcaatac
    38161 tcctgccagc cctgtacgtc gccgtacgcg tgggtaaaat catgatgcat acgcaagaaa
    38221 tactcttcgt tcaggcgttc aagacggatc tcaaacggat cttctaccac cgcaatcgcc
    38281 gcctgagtca ttcgctcgcg caggcattcc ggcaggtgat tcgaaccgat catccggctt
    38341 tcatcttcca gcacccacag gtgcaaatcc tgacgggcgt cggtttttag catttcggca
    38401 gccagcaggt tttcaaaact cgcctggcta agttgtggtt gtaacgtgcg gccaaacgcc
    38461 gaaccgcgat gacgcgccag cccttccaga tcaacaccgt tcggctgttg ctgcactaac
    38521 agcgttttac cgctgccggt acaaccgcca atcagcacta tcggtttttg tgccagttca
    38581 atggtcgcct gaatcgcggt ctggcgcagt gccttataac cgccgtctac cagcggataa
    38641 tcaatccccg cttcatgcaa ccagcgttgc acaatatgtg aacgttgacc gccgcgggcg
    38701 cagcagagaa taccttgcgg attttgcagg cacgctgccc gccaggcgtc catacgctgc
    38761 tgacgaattt cacccgccac cagtttatga cccagcgcca gcgccgcgtc tgacccttgc
    38821 tgtttataac aggtgccaac ggcggcgcgt tcatcgttat tcattaacgg caggttgata
    38881 gcggcaggca ttgcgccttg ctcaaactcg atgggggcgc gaacatcaat aatgggcgta
    38941 tcagcaatca gcagggcacg atagtcctgt tccgtgtgtc tctcttgcat agttaaaagt
    39001 gaacctcaaa tcagcttgca cgctatttta cgcgccaacg cgcaaggaaa cttgattttt
    39061 aactgcgtgg gttgccgaaa atttctaaaa atccgctgat ttccggcctg cgctgggtaa
    39121 acaaggtcac aatatcttct accgctttgc cgctgccaaa tttgcgccat gccagactca
    39181 atggcgaagg cgggcgcatc gttgggatta cccggctgac cagttgttga ttatcgatca
    39241 ttgactggca aagtgatttt ggcaaaaaac caatgccaac gcccgccaga tgggcggcga
    39301 ttttcgtttc catatcagga acaataatct ctttttgccc tggcaatcgc caggcgacgc
    39361 gtttggttaa ggtgcgggcg ctgtcttcaa tattgaccgc cggaaagcga cgcaactgag
    39421 cttctgttag cggctcttca acgttcgcca gcggatgatc cgccgccatg acaaagcgcc
    39481 attgcaccga tcctaaggga tcaagactaa aggtatttgc cagcgcctcg gttcccgtga
    39541 cgccgatagc cagtgaaaaa ccttcgtaca atagcgagtc ccagacgccc atatagattt
    39601 gccgggagat gtggaactgg gtaaaggggt aacgttcatt cagccacgcc agcagctggg
    39661 cgacggcctg gggattgtag agcaggttgt tgatgacaat attcacctgg cgttccacgc
    39721 catcattcac ctgttgcagc tcgctgggca tactttccag ccagctcagc cagtctctgg
    39781 cctgagaaag tagatgctcg ccagccgctg tcaacgtcac gctgcgagtc gtgcggaaaa
    39841 acagcgctac tccggtattc tcttccagaa gtttaatgcg ataactgatc gtcgccgtgg
    39901 ttttacataa tcgttctgcc gcttttgaaa aacttcctgt ttcagcaacc gcaatgaaag
    39961 tccgcaaggt ttctggatcg aacatcttca ggtatcccct tttaaatccg caagttgcgt
    40021 gattttctta tcctctgatt tatcagtatt tttacatgat aaccctgttc aatttgtgga
    40081 ctaaatctag ttttggaaaa atattccaac ttttgtgttg atgttgttct cttaaggttt
    40141 tagattgcct gttattgaaa ccaagctgac cggtcggcgg tggttgaatg gaattatgtt
    40201 acaaggacaa aaagatgaaa cttcaggtat taccgttaag tcaggaagcc tttagtgctt
    40261 atggcgacgt aatcgaaacg cagaaacggg attttttcca tattaacaat ggactggtgg
    40321 agcgttacca cgatttggcg ctggttgaga ttcttgagca agaccgtacg cttatcagca
    40381 ttaaccgcgc gcaaccggcg aatctgccgc tgaccattca cgaactcgaa cgtcatccgc
    40441 tgggtactca ggcctttatc ccgatgaaag gtgaagtgtt tgtggtggtc gtggcgttag
    40501 gtgacgacaa accagacctg tcaacgctgc gggcgtttat taccaacggc gaacaggggg
    40561 tgaattacca tcgtaacgtc tggcatcatc cacttttcgc ctggcagcgc gtcaccgatt
    40621 ttctgaccat cgatcgcggc ggcagtgaca attgtgatgt tgaaagtatt cctgaacagg
    40681 aactctgttt tgcgtgacgc ctgcaaccga cttgcataag ataaactaat tgttcattgt
    40741 ttatgctcac ttgtaggtcg gagttaacgt aggtatgacg gaagttagac ggcgcggcag
    40801 gccaggacag gcggagcctg tggcacagaa gggcgcacag gcgttagagc ggggaattgc
    40861 gattctgcaa tatctggaaa aaagtggggg aagttcatcg gttagcgata tttctctcaa
    40921 tctggatttg ccgctctcca cgacctttcg cttgctgaag gttttacagg cagcggattt
    40981 tgtctaccag gacagtcagt taggctggtg gcatatagga ttaggtgttt ttaacgtcgg
    41041 tgcggcgtac atccataacc gcgatgtcct ctccgtcgcc gggccgttta tgcgccgcct
    41101 gatgttactt tccggcgaaa cggtcaatgt cgcgatccgt aacggcaatg aagcagtatt
    41161 aattggtcag ttagagtgta aatcgatggt caggatgtgt gcgccgctgg gcagtcgtct
    41221 gccgctgcat gcttccggtg cgggcaaggc gctgctttat ccgctggcgg aagaggagtt
    41281 gatgagcatc attctgcaaa ccggtttgca gcagtttacg ccaacgacgc ttgtggatat
    41341 gcccaccttg ctgaaggacc tggaacaagc gcgtgaactg ggctataccg tagataaaga
    41401 agagcatgtt gtaggcctga attgcatagc ttcagcaatt tacgatgatg taggtagtgt
    41461 tgttgccgct atctccatct ccgggccttc atcaagactg acagaagatc gttttgtcag
    41521 tcagggtgag ctggtcagag acaccgcccg cgatatcagc acggcgttgg gactgaaagc
    41581 acatccatag tgtctgtcgc atcccgctct gcggagcggg tttttttgac aaaatttgaa
    41641 agttggaaaa tttttccaat taatagaggt aggaataaaa tggcaaaaat gagagccgtt
    41701 gacgcggcaa tgtatgtgct ggagaaagaa ggtatcacca ccgccttcgg cgttccggga
    41761 gctgcaatca atccgttcta ttcagcgatg cgtaagcacg gcggtattcg tcacattctg
    41821 gcgcgtcatg tggaaggtgc ttcgcacatg gcggaaggtt atacccgcgc aacggcaggg
    41881 aatatcggcg tatgtctggg gacttccggt cctgcgggca cggacatgat caccgcgctc
    41941 tattccgctt ctgctgattc cattcctatt ctgtgcatta ccggccaggc accgcgcgcc
    42001 cgtctgcata aagaagattt tcaggccgta gatattgaag caattgctaa accggtcagc
    42061 aaaatggcgg ttacagttcg tgaagcggcg ctggtgcctc gcgtgctgca acaggcgttt
    42121 cacctgatgc gttctggtcg cccgggtccg gtgctggtgg atttaccgtt cgacgttcag
    42181 gttgcggaaa tcgagtttga tcctgacatg tacgaaccgc tgccggtcta caaacctgct
    42241 gccagccgta tgcagatcga aaaagctgta gaaatgttaa ttcaggccga acgtccggtg
    42301 attgttgccg ggggcggggt aatcaatgct gacgctgctg cactgttaca acagtttgct
    42361 gaactgacca gcgttccggt gatcccaacg ctgatgggct ggggctgtat cccggatgac
    42421 cacgaactga tggccgggat ggtgggtctg caaaccgcgc atcgttacgg taacgcaacg
    42481 ctactggcgt ccgacatggt gtttggtatc ggtaaccgtt ttgcgaaccg tcataccggt
    42541 tcggtagaga aatacaccga agggcgcaaa atcgttcata tcgatattga gccgacgcaa
    42601 attggccgcg tgctgtgtcc ggatctcggc attgtctctg atgctaaagc ggcgctgaca
    42661 ctgctggttg aagtggcgca ggagatgcaa aaagcgggtc gtctgccgtg tcgtaaagaa
    42721 tgggtcgccg actgccagca gcgcaaacgc actttgctgc gcaaaaccca cttcgacaac
    42781 gtgccggtga aaccgcagcg cgtgtatgaa gagatgaaca aagcctttgg tcgcgatgtt
    42841 tgttatgtca ccaccattgg tctgtcacaa atcgctgcgg cacaaatgct gcatgtcttt
    42901 aaagaccgcc actggatcaa ctgtgggcag gcaggcccgt tgggctggac cattccggcg
    42961 gcgctgggcg tgtgtgccgc tgatccagag cgcaaagtgg tggcgatttc cggtgacttc
    43021 gacttccagt tcctgattga agaattagcc gttggcgcac agttcaacat tccgtacatc
    43081 catgtactgg tcaataacgc ctatctgggt ctgattcgcc agtcgcagcg cgcgtttgat
    43141 atggattact gcgtacagct cgcgttcgaa aatattaact ccagcgaagt gaacggttac
    43201 ggcgtcgacc acgtaaaagt agcggaaggt ttgggttgta aagcgattcg cgttttcaaa
    43261 ccggaagata tagcaccagc ctttgaacag gcgaaagcct taatggcgca atatcgggta
    43321 ccggtagtcg tggaagttat tctcgagcgt gtgaccaata tttcgatggg cagcgaactg
    43381 gataatgtca tggaatttga agatatcgcc gataacgcag tggatgcacc aactgaaacc
    43441 tgcttcatgc actatgaata agggagataa ataatgttac gtttctctgc taatttatcg
    43501 atgttatttg gtgaatatga ttttctcgcc cgttttgaga aagctgcgca gtgtggtttt
    43561 cgcggcgttg aatttatgtt cccttatgac tacgacattg aagaattaaa acaggtgctg
    43621 gcgagtaata aactcgaaca tacgctgcac aatttaccgg caggtgactg ggcggcgggc
    43681 gagcggggta ttgcctgtat tcctggccgt gaagaagagt ttcgggatgg cgtagcagca
    43741 gcgattcgtt atgcccgtgc gctgggtaat aaaaaaatta actgtctggt cggtaaaacg
    43801 ccggctggtt tcagcagtga acagattcac gcaacgcttg tagaaaacct gcgttatgcc
    43861 gcgaatatgc tgatgaaaga agatatttta ttactgattg aacctattaa ccattttgat
    43921 attcctggtt tccatctcac cggaactcgg caggcgctga aattgattga tgatgttggt
    43981 tgctgcaatt tgaaaattca gtatgacatt tatcacatgc agcggatgga aggtgaatta
    44041 accaacacta tgacgcaatg ggccgataaa attggtcacc tgcaaattgc cgataatccg
    44101 catcgcggcg aaccggggac cggagaaatt aattatgatt atctctttaa ggtaatcgaa
    44161 aattctgact acaacggttg ggttgggtgt gaatataaac cccaaaccac cacggaagcc
    44221 ggtttacgct ggatggatcc gtaccgttaa aacgtaacgc tattcagaca atgctttttt
    44281 aggccgctaa gttggcaggg gatcgtgttg tctgaattca ggaaaagcga aatttaaaag
    44341 aggttaatta tgaaactggg atttattggc ttaggcatta tgggtacacc gatggccatt
    44401 aatctggcgc gtgccggtca tcaactgcat gtcacaacca ttggtccggt tgctgatgaa
    44461 ttactgtcac tgggtgccgt cagtgttgaa actgcgcgcc aggtaacgga agcatcggac
    44521 atcattttta ttatggtgcc ggatacgcca caggttgaag aagttctgtt cggtgaaaat
    44581 ggttgtacca aagcctcgct gaagggcaaa accattgttg atatgagctc catttccccg
    44641 attgaaacta agcgtttcgc tcgtcaggtg aatgaactgg gcggcgatta tctcgatgcg
    44701 ccagtctccg gcggtgaaat cggcgcgcgt gaagggacgt tgtcgattat ggttggcggt
    44761 gacgaagcgg tatttgaacg tgttaaaccg ctgtttgaac tgctcggtaa aaatatcacc
    44821 ctcgtgggcg gtaacggcga cggtcaaacc tgcaaagtgg caaatcagat tatcgtggcg
    44881 ctcaatattg aagcggtttc tgaagccctg ctgtttgctt caaaagccgg tgcagatccg
    44941 gtacgtgtgc gccaggcgct gatgggcggc tttgcttcct cacgtattct ggaagttcac
    45001 ggtgagcgca tgattaaacg cacctttaat ccaggtttta aaattgcgct gcaccagaaa
    45061 gatctcaacc tggcactgca aagtgcgaaa gcactggcgc tgaacctgcc aaacaccgcg
    45121 acctgccagg aattgtttaa tacctgtgcg gcaaacggtg gcagccagtt ggatcactct
    45181 gcgttagtgc aggcgctgga attaatggct aaccataaac tggcctgata cccgcaataa
    45241 aaatggccga tatcagaaaa tgaatcggcc agcaatatta aaaaaagaaa gcagccaaag
    45301 atgttgcttc agtattaaaa ataatatttt tattttattt gttcctcata gctagattaa
    45361 aacagcgtta ttcgatacgt gaaattaaga gggatttatg gaacatcaga gaaaactatt
    45421 ccagcaacgc ggctatagcg aagatctatt gccaaaaacg caaagccagc ggacctggaa
    45481 aacatttaac tattttacct tatggatggg ttcggttcat aacgttccca attatgtgat
    45541 ggtcggcggc ttttttattc tcggcttgtc tacctttagt attatgctgg caattatcct
    45601 cagcgccttt ttcattgccg cggtaatggt attaaacggt gctgcgggca gtaaatacgg
    45661 tgtgcctttt gccatgatcc tgcgtgcttc ttacggcgta cgtggtgcac tgtttcccgg
    45721 attattaaga ggcgggattg ctgccattat gtggtttggc ctgcaatgtt acgctggatc
    45781 gctggcctgc ttgatcctga ttggcaaaat ctggccggga tttttaactc tcggtggtga
    45841 ttttaccctg ttagggcttt ctctaccggg cttaattact ttcttactct tctggctggt
    45901 caacgtcgga atcggtttcg gtggtggcaa agttttaaat aaattcactg ccattcttaa
    45961 cccgtgcatc tatatcgttt tcggcggtat ggcgatttgg gcgatttcgc tggtcgggct
    46021 cggtccaatc tttgactaca ttccgagcgg tattcagaaa gcagaaaaca gtggcttcct
    46081 gttcctggtg gtgattaacg cggtagttgc ggtctgggcg gcaccggcag tgagcgcatc
    46141 cgactttacg caaaacgccc actcgtttcg tgagcaggcg ctggggcaaa cgctcggttt
    46201 agttgtggcc tatattctgt ttgcggtcgc cggggtatgt attattgccg gagccagtat
    46261 tcactacggc gctgacacct ggaacgtgct ggatattgtt cagcgttggg atagcctgtt
    46321 tgcctcgttc tttgcggtac tggttattct gatgacgacc atttccacta acgccaccgg
    46381 taatattatt ccggctggtt atcagattgc cgctattgca ccgacgaaac tgacctataa
    46441 aaacggcgta ctgattgcca gtattatcag cctgctgatc tgcccgtgga aattaatgga
    46501 aaatcaggac agtatttatc tgttcctcga tattatcggc ggaatgctgg gtccggtaat
    46561 tggtgtcatg atggcgcatt attttgtggt gatgcgtggg caaattaatc ttgatgaact
    46621 gtacaccgca cctggcgatt ataaatatta cgataacggt tttaacctca ctgcgttttc
    46681 agtaactctg gtggccgtta ttttatctct tggcggtaag tttattccct ttatggaacc
    46741 gttatctcgt gtttcatggt ttgtcggcgt catcgtcgcc tttgcggcct acgccttatt
    46801 aaagaaacgt actacagcag aaaacacagg agaacaaaaa gtcacaggtt aattaatccc
    46861 gatattgaac att
//
LOCUS       DACBVI010000019        46701 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.019, whole genome shotgun sequence.
ACCESSION   DACBVI010000019 DACBVI010000000
VERSION     DACBVI010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.019"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..308)
                     /locus_tag="IU325_05335"
     CDS             complement(<1..308)
                     /locus_tag="IU325_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP5808466.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     "
     gene            507..1205
                     /locus_tag="IU325_05340"
     CDS             507..1205
                     /locus_tag="IU325_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HAP5808467.1"
                     /translation="MIFQKIARLLKSEINGYSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPPIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVAHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(1250..2149)
                     /gene="eamA"
                     /locus_tag="IU325_05345"
     CDS             complement(1250..2149)
                     /gene="eamA"
                     /locus_tag="IU325_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HAP5808468.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            2344..3531
                     /gene="ydeE"
                     /locus_tag="IU325_05350"
     CDS             2344..3531
                     /gene="ydeE"
                     /locus_tag="IU325_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HAP5808469.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETVSVWTPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGIVLTSLPPFSLFIILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            3658..3753
                     /gene="mgtS"
                     /locus_tag="IU325_05355"
     CDS             3658..3753
                     /gene="mgtS"
                     /locus_tag="IU325_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HAP5808470.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(3951..4862)
                     /gene="dgcZ"
                     /locus_tag="IU325_05360"
     CDS             complement(3951..4862)
                     /gene="dgcZ"
                     /locus_tag="IU325_05360"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HAP5808471.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGIRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRVSAPFNIH"
     gene            complement(5117..5509)
                     /gene="ydeI"
                     /locus_tag="IU325_05365"
     CDS             complement(5117..5509)
                     /gene="ydeI"
                     /locus_tag="IU325_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HAP5808472.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKKMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            complement(5874..7919)
                     /gene="dcp"
                     /locus_tag="IU325_05370"
     CDS             complement(5874..7919)
                     /gene="dcp"
                     /locus_tag="IU325_05370"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HAP5808473.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMLQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQYNQRLLAANKSGGLVVNDFAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNV"
     gene            8056..8802
                     /gene="ydfG"
                     /locus_tag="IU325_05375"
     CDS             8056..8802
                     /gene="ydfG"
                     /locus_tag="IU325_05375"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HAP5808474.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            8891..9577
                     /locus_tag="IU325_05380"
     CDS             8891..9577
                     /locus_tag="IU325_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HAP5808475.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLEIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            9754..9957
                     /gene="ydfZ"
                     /locus_tag="IU325_05385"
     CDS             9754..9957
                     /gene="ydfZ"
                     /locus_tag="IU325_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HAP5808476.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(9993..11453)
                     /locus_tag="IU325_05390"
     CDS             complement(9993..11453)
                     /locus_tag="IU325_05390"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HAP5808477.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVVADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEENVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYHHAAYALMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(11542..12909)
                     /locus_tag="IU325_05395"
     CDS             complement(11542..12909)
                     /locus_tag="IU325_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5808478.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFQLYSLGAALVFHE
                     IFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTT
                     LIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVA
                     MGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVF
                     EKVNDSNQPTAKLAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNK
                     AIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYA
                     PSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPIL
                     AGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQ
                     PSRSL"
     gene            complement(12967..13986)
                     /locus_tag="IU325_05400"
     CDS             complement(12967..13986)
                     /locus_tag="IU325_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HAP5808479.1"
                     /translation="MKSILIEKPNQLAIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHMFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(13998..15212)
                     /gene="rspA"
                     /locus_tag="IU325_05405"
     CDS             complement(13998..15212)
                     /gene="rspA"
                     /locus_tag="IU325_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HAP5808480.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(15418..15744)
                     /locus_tag="IU325_05410"
     CDS             complement(15418..15744)
                     /locus_tag="IU325_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HAP5808481.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALIWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRA"
     gene            15879..16220
                     /locus_tag="IU325_05415"
     CDS             15879..16220
                     /locus_tag="IU325_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HAP5808482.1"
                     /translation="MKITLSKRIGLLAFLLPCAMALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            16255..16815
                     /gene="speG"
                     /locus_tag="IU325_05420"
     CDS             16255..16815
                     /gene="speG"
                     /locus_tag="IU325_05420"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HAP5808483.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(16818..17528)
                     /locus_tag="IU325_05425"
     CDS             complement(16818..17528)
                     /locus_tag="IU325_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HAP5808484.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAQT
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            17636..17941
                     /locus_tag="IU325_05430"
     CDS             17636..17941
                     /locus_tag="IU325_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HAP5808485.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            18140..20566
                     /locus_tag="IU325_05435"
     CDS             18140..20566
                     /locus_tag="IU325_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAP5808486.1"
                     /translation="MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGNDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     RTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            20627..23050
                     /locus_tag="IU325_05440"
     CDS             20627..23050
                     /locus_tag="IU325_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAP5808487.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAETPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIHLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLATIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            23061..23678
                     /gene="dmsB"
                     /locus_tag="IU325_05445"
     CDS             23061..23678
                     /gene="dmsB"
                     /locus_tag="IU325_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HAP5808488.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            23680..24534
                     /locus_tag="IU325_05450"
     CDS             23680..24534
                     /locus_tag="IU325_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HAP5808489.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VTIIGKMPQALGKLWLLVSMALGVIFVWMMTGVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCTGLGCWLCPLIRRREPHVAGLVLGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            24577..25191
                     /gene="dmsD"
                     /locus_tag="IU325_05455"
     CDS             24577..25191
                     /gene="dmsD"
                     /locus_tag="IU325_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HAP5808490.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTRDGWET
                     QWPLPEASLAPLVTAFQTQSEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMQQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKSLFR"
     gene            25349..26641
                     /gene="clcB"
                     /locus_tag="IU325_05460"
     CDS             25349..26641
                     /gene="clcB"
                     /locus_tag="IU325_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HAP5808491.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFVQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLFAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(26594..27289)
                     /gene="bioD"
                     /locus_tag="IU325_05465"
     CDS             complement(26594..27289)
                     /gene="bioD"
                     /locus_tag="IU325_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAP5808492.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(27414..28634)
                     /gene="mlc"
                     /locus_tag="IU325_05470"
                     /gene_synonym="dgsA"
     CDS             complement(27414..28634)
                     /gene="mlc"
                     /locus_tag="IU325_05470"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HAP5808493.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEEAQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(28769..29662)
                     /locus_tag="IU325_05475"
     CDS             complement(28769..29662)
                     /locus_tag="IU325_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP5808494.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLVDSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            29769..31022
                     /locus_tag="IU325_05480"
     CDS             29769..31022
                     /locus_tag="IU325_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5808495.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            31419..31754
                     /locus_tag="IU325_05485"
     CDS             31419..31754
                     /locus_tag="IU325_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HAP5808496.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQP
                     AKPAAQPAA"
     gene            31847..31930
                     /locus_tag="IU325_05490"
     CDS             31847..31930
                     /locus_tag="IU325_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808497.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            32030..32851
                     /locus_tag="IU325_05495"
     CDS             32030..32851
                     /locus_tag="IU325_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HAP5808498.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDRLDQLSQK"
     gene            complement(32890..33219)
                     /gene="mdtI"
                     /locus_tag="IU325_05500"
     CDS             complement(32890..33219)
                     /gene="mdtI"
                     /locus_tag="IU325_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HAP5808499.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(33206..33571)
                     /gene="mdtJ"
                     /locus_tag="IU325_05505"
     CDS             complement(33206..33571)
                     /gene="mdtJ"
                     /locus_tag="IU325_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HAP5808500.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            33983..35017
                     /gene="tqsA"
                     /locus_tag="IU325_05510"
     CDS             33983..35017
                     /gene="tqsA"
                     /locus_tag="IU325_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HAP5808501.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQAIEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(35042..36430)
                     /gene="pntB"
                     /locus_tag="IU325_05515"
     CDS             complement(35042..36430)
                     /gene="pntB"
                     /locus_tag="IU325_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HAP5808502.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTSIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(36441..37973)
                     /gene="pntA"
                     /locus_tag="IU325_05520"
     CDS             complement(36441..37973)
                     /gene="pntA"
                     /locus_tag="IU325_05520"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HAP5808503.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            38497..39441
                     /gene="ydgH"
                     /locus_tag="IU325_05525"
     CDS             38497..39441
                     /gene="ydgH"
                     /locus_tag="IU325_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAP5808504.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            39627..41009
                     /locus_tag="IU325_05530"
     CDS             39627..41009
                     /locus_tag="IU325_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP5808505.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            41046..41768
                     /gene="folM"
                     /locus_tag="IU325_05535"
     CDS             41046..41768
                     /gene="folM"
                     /locus_tag="IU325_05535"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HAP5808506.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLTDVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(41765..42100)
                     /locus_tag="IU325_05540"
     CDS             complement(41765..42100)
                     /locus_tag="IU325_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HAP5808507.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLASLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            42229..42948
                     /gene="rstA"
                     /locus_tag="IU325_05545"
     CDS             42229..42948
                     /gene="rstA"
                     /locus_tag="IU325_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HAP5808508.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDHAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            42952..44253
                     /gene="rstB"
                     /locus_tag="IU325_05550"
     CDS             42952..44253
                     /gene="rstB"
                     /locus_tag="IU325_05550"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HAP5808509.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     VAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKMVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            44330..45259
                     /locus_tag="IU325_05555"
     CDS             44330..45259
                     /locus_tag="IU325_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HAP5808510.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAELEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQRINQLKTTFERIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IATLPQDAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(45256..46659)
                     /gene="fumC"
                     /locus_tag="IU325_05560"
     CDS             complement(45256..46659)
                     /gene="fumC"
                     /locus_tag="IU325_05560"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HAP5808511.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELAMGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSETEF
                     DSWVRPEQMVGSMKAGR"
BASE COUNT        11853 a        11911 c        11090 g        11847 t
ORIGIN      
        1 tcttgcagta gtttcatcgc aaattcaaga actgcctgtc cgttcatggt gccatagtct
       61 ttcatctcga taacagctac aggcattttc ccctgtgcaa cagcttcaat ctctggtttc
      121 tgaaagcgaa cttgcgggcc aagtaaaaca acgtcaactt cgttattttt aattttccct
      181 ttagcctctg caatcgccaa tgcggaaata ttaacttcaa gtgaaatagc gttagcatga
      241 tcaatcatac gtttaaccag catactggtt gacatgcccg cagcgcaaac taacaatatc
      301 tttttcatat tttcccttac tggtatataa cagattacat tgtgtaaata tcatatcccg
      361 cagtagctat acgtcctggt acattgatca cagtaataca gcactaaagt tgttcaacat
      421 cagggtgata attaaacgac tcgaccatac gataaaccaa tcaggactca actccttgtt
      481 ttcaatggct cttaaaaggt gcaaaagtga tttttcaaaa gattgcccgc ttacttaaat
      541 ccgagatcaa tggctattca tggcatgttg gtgatttgtt gccgtcagaa gctgaactcg
      601 ctgttcgtta taatgtttcg cgtaataccc tgcgtaaggc attatccttg ctggaaggcg
      661 aaggaattat tcatagaaag catggttcag gaacatatat tcaaaaaaag aattttgttg
      721 cacacattga tcacatgaac agcttcagtg aaattgcgca taaaagcggc aaggaggcag
      781 gaagtcagat tatgaaattt gaagtgcagg atgcttctcc ccctattgcg actgagctga
      841 atttagtgac tggtgagcag gtttattaca taaagagact gcgattcatt gaggataatg
      901 cagcacaatt agaagaaacg tggatgtcag tggcacgttt tcctgattta accgtagcac
      961 atatgcaaaa atccaaattt tcgtatattg agaacgaatg cgggatcaaa atcattggca
     1021 cctttgaaac tttctcccct acttttccta ccccagaaat cgccagtatt ttacggatca
     1081 gcccacggga tcccatactt aaaattcaga cccaggctgt ggacagtaac tctattccgc
     1141 tggattattc gttactttac agcaatattt tcgagttcca ggtaaagtac tttttcccgc
     1201 gataaaagca gacgcacaga gtgctgacat cacattctgt gcgtatggtt tagcctctaa
     1261 cctttaccgc tttacgccac cgcaagccaa atacattgat atacagcccg gtcataatga
     1321 gcaccgcccc gaaaaattgt agacccgtta agcgttcatc caacaatagt gccgcacttg
     1381 ccagtcctac tacgggcacc agtaacgata acggtgcaac ccgccaggtt tcatagcgtc
     1441 ccagtaacgt cccccagatc ccataaccaa caattgtcgc cacaaacgcc agatacatca
     1501 gagacaagat ggtggtcata tcgatagtaa ccagactgtg aatcatggtt gcggaaccat
     1561 cgagaatcag cgaggcaaca aagaagggaa tgattgggat taaagcgctc cagattacca
     1621 gcgacatcac cgccggacgc gttgagtgcg acatgatctt tttattgaag atgttgccac
     1681 acgcccaact aaatgctgcc gccagggtca acataaagcc gagcatcgcc acatgctgac
     1741 cgttcagact atcttcgatt aacaccagta cgccaaaaat cgctaaggcg atccccgcca
     1801 attgtttgcc atgcagtcgc tcaccgaaag taaacgcgcc aagcacgata gtaaaaaacg
     1861 cctgtgcctg taacaccagc gaagccagtc cagcaggcat accgaagtta atggcacaaa
     1921 aaagaaaagc aaactgcgca aaactgatgg ttaatccata ccccagcagc aaattcagcg
     1981 gtactttcgg tcgtgcgaca aaaaagatag ccggaaaagc aaccagcata aagcgcaaac
     2041 cggccaacat cagcggtggc atgttatgaa gccccacttt gatgaccaca aaatttagcc
     2101 cccatacgac cactaccagt agcgccaaca ccccatcttt tcgcgacatt ctaccgcctc
     2161 tgaatttcat cttttgtaag caatcaactt agctgaattt acttttcttt aacagttgat
     2221 tcgttagtca ccgtttacga cggcattaat gcgcaaataa gtcgctatac ttcggatttt
     2281 tgccatgcta tttctttaca tctctaaaac aaaacataac gaaacgcact gccggacaga
     2341 caaatgaact tatccctacg acgctctacc agcgcccttc ttgcctcgtc gttgttatta
     2401 accatcggac gcggcgctac gctgccgttt atgaccattt acttgagtcg ccagtacagc
     2461 ctgagtgtcg atctaatcgg ttatgcgatg acaattgcgc tcactattgg cgtcgttttt
     2521 agtctcggtt ttggtatcct ggcggataag ttcgacaaga aacgctatat gttactggca
     2581 attaccgcct tcgccagcgg ttttattgcc attcctttag tgaataacgt gacgctggtg
     2641 gtgctctttt ttgccctcat taactgcgcc tattccgttt ttgctaccgt gttgaaagcc
     2701 tggtttgccg acaatctttc ctccaccagc aaaacgaaaa tcttctcaat caactacacc
     2761 atgctcaaca ttggctggac catcggtccg ccgctcggca cgctgttggt gatgcagagc
     2821 atcaatctgc ccttttggct ggcagctatc tgttccgcgt ttcccatgct tttcattcaa
     2881 atttgggtaa agcgcagcga gaaaatcatc gccacggaaa cagtcagtgt ctggacgccg
     2941 aaagttttat tacaagataa agcactgttg tggtttacct gctctggttt tctggcttcc
     3001 tttgttagcg gcgcatttgc ttcatgcatt tcacaatatg tgatggtgat tgctgatggt
     3061 gattttgccg aaaaggtggt tgcggttgtt cttccggtta atgctgccat ggtagttacg
     3121 ttgcaatatt ccgtgggacg ccgacttaac ccggctaaca tccgcgcgct gatgacagca
     3181 ggcaccctct gtttcgtcat cggtctggtt ggttttattt tttccggcaa cagcctgcta
     3241 ttgtggggta tgtcagctgc ggtatttact gtcggtgaaa tcatttatgc gccgggcgag
     3301 tatatgttga ttgaccatat tgcgccgcca ggaatgaaag ccagctattt ttccgctcag
     3361 tctttaggct ggcttggtgc ggcgattaac ccattagtga gtggcatagt gcttaccagt
     3421 ctgccgcctt tctcgctgtt tatcatctta gcgttggtga tcattgctgc gtgggtgctg
     3481 atgttaaaag gaattcgtgc aagaccgtgg gggcagcccg cgctttgttg atttaagtcg
     3541 aacacaataa agatttaatt catccttcgt ttaggttacc tctgctaata tctttctcat
     3601 tgagatgaaa attaaggtaa gcgaggaaac acaccacacc ataaacggag ggaaataatg
     3661 ctgggtaata tgaatgtttt tatggccgta ctgggaataa ttttattttc tggttttctg
     3721 gccgcgtatt tcagccacaa atgggatgac taatgaacgg agataatccc tcacctaacc
     3781 ggccccttgt tacagttgtg tacaaggggc ctgattttta tgacggcgaa aaaaaaccgc
     3841 cagtaaaccg gcggtgaatg cttgcatgga tagatttgtg ttttgctttt acgctaacag
     3901 gcattttcct gcactgataa cggatcgttg acacagtagc atcagttttc tcaatgaatg
     3961 ttaaacggag ctgaaactcg gttaatcaca ttttgttcgt caataaacat gcagcgattt
     4021 cttccggttt gcttaccctc atacattgcc cggtccgctc ttccaatgac cacatccaga
     4081 ggctcttcag gaaatgcgcg actcacacct gcagtcacgg taatgttgat atgcccttca
     4141 gaatgtgtga tggcatggtt atcgactaac tggcaaattc tgatacctgc acgacatgct
     4201 tcttcatcat tagccgcttt gacaataatg ataaattctt cgccaccgta gcgataaacc
     4261 gtttcgtaat cgcgcgtcca actggctaag taagttgcca gggtgcgtaa tactacatcg
     4321 ccgattaaat gcccgtaggt atcattaacc aatttaaatc ggtcaatatc caacaacatt
     4381 aaataaagat tcagaggctc agcgttgcgt aactgatgat caaaggattc atcaagaacc
     4441 cgacgacccg gcaatcccgt caaaacatcc atattgctac ggatcgtcag caaataaatt
     4501 ttgtaatcgg ttaatgccgc agtaaaagaa agcaaccctt cctgaaaggc gtcgaaatgc
     4561 gcgtcctgcc agtgattttc aacaatagcc aacattaatt cccgaccaca gttatgcata
     4621 tgttgatggg cagaatccat tagccgaacg taaggtaatt catcgttatc gagtggtccc
     4681 agatgatcaa tccaccgacc aaactggcac agtccataag aatggttatc cgttatttct
     4741 ggcttactgg catctctcgc gaccacgctg tgaaacatac tcaccagcca ctggtagtgg
     4801 gcatcgatag ccttattgag atttaacaag atggcatcaa tttccgttgt cttcttgatc
     4861 attgccactc ctttttcaca gttccttgtg cgcgctattc taacgagaga aaagcaaaat
     4921 tacgtcaata ttttcataga aatccgaagt tatgagtcat ctctgagata acattgtgat
     4981 ttaaaacaaa atcagcggat aaaaaagtgt ttaattctgt aaatcacctc tgcattatcg
     5041 taaataaaag gatgacaaat agcataaccc aataccctaa tggcccagta gttcaggcca
     5101 tcaggctaat ttatttttat ttctgcaaat gagtgacccg aacgacggcc ggcgcgcttt
     5161 tcttatccag actgccgcta atgttgatca tctggtccgg ctgaacttct cgtccatcaa
     5221 agacggccgc aggaataacg acattaattt caccgctctt atcgcgaaaa acgtaacggt
     5281 cctctccttt gtgagaaatc aaattaccgc gtagtgaaac cgaagcgcca tcgtgcattt
     5341 tttttgcgaa atcaacggtc attttttttg catcatcggt tccgcgatag ccatcttcta
     5401 ttgcatgagg cggcggtggc gctgcatcct gttttaatcc gccctggtca tctgccaacg
     5461 cataaggcat gacaagaaaa cttgctaata caatggcctg aaatttcata ctgactcctt
     5521 aattgcgttt ggtttgactt attaagtctg gttgctattt ttataattga caaataagaa
     5581 tactgccaat tgttataagg catttaaaat caggcaacta gctgtcaaat attcagagag
     5641 ttttactcac taaagtaaga agaagattaa agagtcttaa acatatttca gaacaatcag
     5701 atttataaat ttgaaaatta ttataacgga agggtataca aaaaaagtaa ttttgctggc
     5761 gactgcgaaa cggtattagc attagcggtg aggtttgccc tcgcccagct gctgcaatta
     5821 ctactctaac caggctggcc tggcgatttc tccaggccag ccattggtgg tgtttatacg
     5881 ttcaagccac gatgttgcag catcggcata atctgaggtg ccttaccgcg ccattgtcga
     5941 tacaggcgtt ccagatcctc gctgttacct ctggaaagga tcgcctcgcg aaaacgcagc
     6001 ccattttcac gcgttaatcc gccctgctca acaaaccact ggtaaccatc atcggccaac
     6061 atttgcgtcc acagataagc gtaataacct gcggcatatc cgccaccaaa aatatgggcg
     6121 aaataactgc tgcgatagcg tggcggtata gcaggaagat ccatattttc cgccaccagc
     6181 gcccgcaatt caaaatcatc gacatcctgc attgcttcgt tttcttccag gcaatgccag
     6241 cgcatatcga gaagtgcggc gctaagcagt tcgctcatct catacccttt gttgaacagg
     6301 ctggcattac gcattttctg ttgcagttcg tcaggcattg ctgccccgct ctgataatgc
     6361 cgggcgtagc gagcgaatac ctgcggatgc gttgcccagt gttcattgat ttgcgacgga
     6421 aattcgacaa aatcacgcgg cgtgttggtg ccggaaagcg tggcataacg ctggcgggca
     6481 aaaaggccgt gcagcgtatg accaaattca tggaataagg ttattacatc atcccagagt
     6541 aacaacgcag gctcaccggc agcgggtttc tgataattgc aaacgttata aattaccggg
     6601 tgcgtttcat taagcgttga ttgctcaaca aaattgccca tccatgcacc gccgcttttt
     6661 gaatcacggg cgaagaaatc accgtaaaat aacgccagcc ccacgccatt atgatcaaaa
     6721 atttcccaca cccgaacgtc aggatggtag acaggaatat caaaacgttc gacaaactta
     6781 ataccgaaga gctgattcgc ggtccagaat acaccttcat tcaacaccgt gtttaattca
     6841 aaatatggct tgagctgcgc ctcatcaaga tcaaatttct cccgccgtac ctgttcggca
     6901 taaaatgccc agtcccacgg ctgcgcgcta aacccgccct gctgcttatc aataaccgcc
     6961 tgtatggagg ctaattcatc gctcgcacgt tgacgcgccg ctggaacaat ttcccgcata
     7021 aagttgagtg ctgcttctgg cgtttttgcc atctgatcgg cgattttcca tgcggcataa
     7081 tgagggaaac caagcagttt cgcctgctgc gcacggatct ccaccagacg ttgaatgata
     7141 gcgcgggtat cattggcatc atttttttcc gctcgcgtcc agcccgcaat aaacagtttt
     7201 tcacgcgtcg cacgatcgcg catttcggca agcgccggtt gttgggtggt attcagcaac
     7261 ggaatcagcc atttgttatc cagacctttc tcgcgagccg cctctgccgc cagagcaatc
     7321 tcttgctcac tcattcctgc cagctgcgcg aaatcgttca caaccagacc gccggattta
     7381 tttgctgcca gtaaccgctg gttatactgg ctggtcaggg tcgcagcttc tgtattcagt
     7441 acttttaatt ttgctttatc agcttgcgca agtttggctc cggcaaggac aaaacgttga
     7501 tgaatcacct ccaccaggcg gatggattca ctatcaagcc ccagggattc tcggcgctgc
     7561 cagacagcat ctacccgcgc gaataattca ccgttcagat agatatcatt agccagttcc
     7621 gccagttcag cggaaaactg ttcatcaaga cgctgtaatt catcattggt atgcgccgca
     7681 gtcatcgcaa aaaagacgct ggtaacgcgg gtaagtaatt ctccgctttg ttccagtgcc
     7741 agaatagtat tgttgaaatc aggcgtttgc gggttaagcg cgatggcagc aatttctgcc
     7801 cgcttttgca gcattccctc atcgaatgcc gggcgatagt gatgattggc aatttgatca
     7861 aaatggggag ccagatacgg cagtgtgctt tgcacaagga aaggattcat tgttgtcatt
     7921 ttcttctcct gaacgcgagg tgttccatag cgtaggctta ctgataacgg agtgcaatct
     7981 tgcaaaccag tattacccgc tcttaagcat cccgtgctat gttattgaca cacaaaagcg
     8041 ttgaggaaca gtgagatgat cgttttagta actggagcaa cggcaggttt tggtgaatgc
     8101 attactcgtc gttttattca acaagggcat aaagttatcg ccactggccg tcgccaggag
     8161 cggttgcagg agttaaaaga cgaactggga gataatcttt atatcgccca actggacgtt
     8221 cgcaaccgcg ccgctattga agagatgctg gcatcgcttc ctgccgagtg gtgcaatatt
     8281 gatatcctgg taaataatgc cggtctggcg ttgggcatgg agcctgcgca taaagccagc
     8341 gttgaagact gggaaacgat gattgatacc aacaacaaag gcctggtata tatgacgcgc
     8401 gccgtcttac cgggtatggt tgaacgtaat catggtcata ttattaacat tggctcaacg
     8461 gcaggcagct ggccgtatgc cggtggtaac gtttacggtg cgacgaaagc gtttgttcgt
     8521 cagtttagcc tgaatctgcg tacggacctg catggtacgg cggtgcgcgt caccgacatc
     8581 gaaccgggtc tggtgggtgg caccgagttt tccaatgtcc gctttaaagg cgatgacggt
     8641 aaagcggaaa aaacctatca aaataccgtt gcattgacgc cagaagatgt cagcgaagcc
     8701 gtctggtggg tgtcaacgct gcctgctcac gttaatatca ataccctgga aatgatgccg
     8761 gttacccaaa gctatgccgg actgaatgtc caccgtcagt aatttttata cccggcgtaa
     8821 ctgccgggtt attgcttgtc acaaaaaagt ggtagactca tgcagttaac tcactcacaa
     8881 gcaagaacga atgaccgtcg aaacgcaact taatcccaca cagcctgtca atcagcaaat
     8941 ttatcgtatc cttcgtcgcg acattgtcca ttgcctgatt gcaccaggca caccgttgtc
     9001 ggaaaaagaa gtttctgttc gtttcaatgt gtcacgccag ccggttcgtg aagcctttat
     9061 taaactggcg gaaaacggcc tgatccaaat tcgtccgcag cgtggcagct acgtcaacaa
     9121 aatttccatg gcccaggtgc gcaacggcag ttttatccgt caggccattg agtgcgcggt
     9181 ggcgcgtcgg gcggcgagca tgattaccga aagccagtgc taccaactgg aacaaaatct
     9241 tcaccagcaa cgcattgcca ttgagcgcaa gcaactggat gatttttttg aacttgatga
     9301 caacttccat caactcctga cccagattgc cgactgtcaa ctggcgtggg ataccattga
     9361 gaacctgaaa gcgaccgttg atcgcgtgcg ctatatgagt ttcgaccacg tttctccacc
     9421 agaaatgctg ttacgccagc atcttgagat tttctctgcc ctgcaaaaac gtgatggcga
     9481 tgcggtagaa cgtgcaatga cgcaacattt gcaggaaatc agcgaatccg tgcgccagat
     9541 ccgtcaggaa aacagcgact ggtttagcga agagtaattc atttcctctc atcccatccg
     9601 ggatgagagt ttttccctcc gtcatatggc tcatgcatgc atcaaaaaag atgtgagctt
     9661 gatcaaaaac aaaaaatatt tcactcgaca ggagtattta tattgcgccc gttacgtggg
     9721 cttcgactgt aaatcagaaa ggagaaaaca cctatgacga cctacgatcg taaccgtaac
     9781 gcaatcacca ctggcagccg tgttatggtt agcggcaccg gtcacactgg caagatcctg
     9841 tcgattgata ctgaaggtct gaccgctgag caaatccgcc gcggaaaaac cgtagttgtt
     9901 gaaggttgtg aagagaaact ggcacctctg gacctgattc gtctcggcat gaactaagcg
     9961 tgtgaatgcc gccgatggcg gcattgcttt ttttacttca cggaatattt cgccacggtc
    10021 gcttttgcac catgcgctaa taaagacaag tacgcttccg tcacttttgc agtaaacaag
    10081 ctattgtctg gcaaatcatc accaaagatc gccttaatcg ccagcaatga ctggacgcgc
    10141 gctttccctt cggcactact ttgtacagcc ttctgaataa caggtaacag tgggtcactg
    10201 atttctatcg gatttccctg ttcatcaaca ccacccacat aacgcatcca acctgcgacg
    10261 cccagcgcca gcagatcgaa cttgctgtca tgcgccagat gccagcgaac agaatctaac
    10321 atccgctgtg gcaatttctg gctgccatcc atcgcaatct gccaggttcg atgacgtagc
    10381 gccgggttgc tatagcgttc aatcaatcgg ttagcgtaat cttgcaaatc aacgccctgc
    10441 actttcaacg tcggcgcttg ttcctgcaac atcaaggcat acgctgcatg atgataatgt
    10501 tcatcttcca tgcagtcatt aatgtgctga tatccggcaa gataccccag atacgccagg
    10561 aatgaatgac tgccgttgag catgcgcaac ttcatctctt cataaggcag cacatcacta
    10621 accagttcgg ctcccgcttt ttcccattcc ggacgtccgg caacaaagtt atcttcgata
    10681 acccactggc ggaaaggttc acaggcaacg cccgcaggat cgcgcacacc ggtaagttgt
    10741 tcgattttcg ccagcgtatc ctccgtcact gcgggcacaa tacggtccac cattgttgat
    10801 gggaaagtca cgttttcttc gatccattgt gccagtttta catcaacagc ttgcgcgtag
    10861 gaagtgacaa cgtcacgcat aacatgaccg ttttctggca tgttgtcaca tgacatgacg
    10921 gtaaatgcgg gaagtcctgc cgctttacgg cgagccagcg cttcgacaat cacccctgtt
    10981 gcagttttcg gctggtgggg attttgtacg tcggcaacga ccatcgggtg atcgagcatt
    11041 aactgtccgg tcgccggaga gtggaaatac cctttttcgg tgattgtcag agagacaatc
    11101 gcgatttgtg gttcacacat cgcagccaac acggtttcta agccatcaat ctgtacgtgc
    11161 aaggcttttt taacgacgcc aacgacgcga gccgtccacg catcggccga catttccgca
    11221 acggtataaa gattatcttg ctgttgtaaa tcggcaattt gctgttcacc gccgattaaa
    11281 ttgacctcat aatatcccca gtcactgaaa tgttccgtag caagaatatc ggcatacacg
    11341 ccctgatgcg cacggtgaaa tgcaccaaag cctaaatgaa caattcttgg ggccaggtta
    11401 ttacgatcat aaacagggag cgtcgctttt gctgataaca aattatttcc cataacaatt
    11461 ccttaaatat aaatatggca agctacatgt tttgttatat gaataaaaat cccctctccg
    11521 gtaagagaag ggattgagga tttaaagact tctggaaggt tgcgcagctc tgacaacacg
    11581 cggttgatct tccgcagcgt cttccagcgc acttaaatca cggtctttca cttctggcat
    11641 tttcagcgca gagattaaac caatcactga atatgccatg atcataatgg cgatcggata
    11701 ccaggattcc gtcatggtgc agaaaatacc cgccaggata ggaccaaaac cggaagcgat
    11761 aagaccacca atttctttag aaatagccat ccgggtaaag cggtttttac agccgaacat
    11821 ttctgccatg gtaatgtttt ccagagcaaa taatcccagc accgcacagt tatgaatcac
    11881 aatcagtgca accataatgg tgctcggggc atagctttta tctacgatga tagaaagcat
    11941 tggccacgcc agcacaatcg cagaggtatt cataataata tacgggatcc ggcgaccaat
    12001 tttatcggat aaccaaccaa ggaacggaat ggtcataaag ccgagaatcg aactgatcat
    12061 caatgcatct gttggaattg ctttgttaaa caataacgtc tgcactaaat agcctgcaag
    12121 gaaagtctga attaaaccgg agttacccgc ctgaccaaaa cgcagccctg ttgccagcca
    12181 gaaggatttg ctctggaaca tgctaccagc aggtgcaagt tttgctgtcg gttggttgct
    12241 gtcgttaacc ttctcaaaga ccgggctttc tttcagattc atacgcaacc agatagcaaa
    12301 gaccatcacg acaacgctcg ccaggaacgg tatacgccat ccccacgcca gcagttcctc
    12361 tttactgaga atgaagaaca taaaggccca gattgccgtt gcgctcaagg ttccgcagtt
    12421 agtccccata gcaacaaatg aggagataat tccgcgctta ccttttggtg catattccgc
    12481 cagcatcgtg ccggcaccgg aaatttccgc gcctgccccc agcccctgaa taatacgcag
    12541 cgtcaccagc aaaattggcg caaacacacc aatttgtgca taggtcggca gcacaccaat
    12601 taaggtggta cagatcccca tcatagtgat ggtaataaag agcacttttt tacgcccgat
    12661 tctgtcgccc attttgccga aaataaatgc tccgacaata cgcgccacat aacctgcacc
    12721 gtaggttccc attgccagaa ttaacgccat tgccgttgat gattcaggaa aaaatatttc
    12781 atgaaacact aacgctgcgc cgagcgaata taactggaaa tccataaatt caagcgcagt
    12841 gcccagccat cccgatactg ctgctttcac caaatcctta gtgcttcttt cgtgtttttc
    12901 tattgtcata atggttatct ctaaaaaaga ggtaagatgc gtactactta ctcgccgtta
    12961 ttggtattat tcagaaaaag tgagtaagac tttgcagcaa tgtttttgat cctgttcaaa
    13021 taaactgatg gcatcagcaa catgctggaa atcaaacata tgggtaatta atttttctgg
    13081 tttaattaac cctttactta accagtcgat aacgaccggg aatttatttg catttaagcg
    13141 tgaagagaaa atagagagtt cttttccggt aattccttgc tgaatcactt cagacggttc
    13201 actggagaag cccatcaata caatacgtgc cgctggagaa gccagcgtta cggcctcttt
    13261 caggatagaa ggatgacaag ccgcatcgat aattaatgtc ggctttatgc ctttttcagc
    13321 gaaaatctcg ccaagcggtg tctggctgtt attaatcgcc cagtcagccc cgctctcttt
    13381 cgctttttcc agtcgttcat caatgcgatc ggcaacaatc acatttttaa cgttatagac
    13441 gccttttaat acctgaacga tcgtcaggcc gattggaccg gcaccgtaaa ccagaacggt
    13501 atcattttca gtcggttgac catgaccggt tacgttcgcc gcaatggtaa aaggttcgat
    13561 catcaccgca tattgatcgg ccactgcttc aggaattttc cacgcatttt ttgccggaac
    13621 cacggcatat tcactgaaac caccgtcagc gtgcacacct aatacagcca gtgtcgtaca
    13681 aacgttcggc ttacctatag agcacggata gcaatgccca cagctgacca ccggatcgac
    13741 agcaacgcgt tcaccgactc tggcgctttc cacgccttca cccactgcat caatgacgcc
    13801 aaagaattca tgaccaatga cgcgcggata tttcgcaaaa ggattatgcc cacgataaat
    13861 atggctatct gaaccacaaa ttccggcaag tttcactttt actcgtactt cacccgctga
    13921 cggggtgggt atttcgcgtt cgataattgc cagttgattc ggtttttcaa ttaaaatgct
    13981 tttcattatc ttactcctta ccagttccac agcgtgccat cttccagacg tgcgactggc
    14041 agataagcag gttcataggg atatttcgcc gccagctttt catcgaattc gatgccaaga
    14101 cccggtttgt ctcccggatg catatagccg ttatcgaaag tccagttgtg cgggaagact
    14161 tcaagcattt gttcggaata acccatgtac tcctggacac cgaaattggg gacccacaga
    14221 tcaaagtgca gcgccgcagc catgcagact ggtgacaaat cggaaggacc gtgtgagcca
    14281 gtacgtacct gatacaacga agcaaaatcg gcaatccggc gcataccggt aattccgcct
    14341 gcatgggtca gcgtggtgcg gatataatcg ataagttgct cttcaatcag ttgcttgcag
    14401 tcccagatgc tgttgaagac ttcacccacc gcgatgggtg tgacggtatg ttggcgaatg
    14461 agacggaagc attcctggtt ttccgcaggc gtcgggtctt ccatccagaa catgcgataa
    14521 tcttcaatgc ttttaccaaa gcgcgccgct tcaataggcg ttaagcgatg gtgcatgtca
    14581 tgcagcaaat gttcattaaa accaaacttg ttacgtaccg cgtcaaacaa tttcggcatg
    14641 aaatcgaggt atttctccgt cgaccacagc tgctcttccg gccactgtcc tttggttgcg
    14701 ggttcataag ccagaccttt acctttcgac atgccgtagg tggttttcat accagggatt
    14761 ccgcactgca cgcggatggc tttgaatccc agctcctgat gacgagcata atcatccaga
    14821 gcttcatcaa tactgtgacc ggtggtatgg caataaacca tcaccccttc acgagacgcg
    14881 ccgccgagta actggtaaag cggcatgttg gcagctttgg ctttaatatc ccacagcgcc
    14941 atatcaaccg ctgaaatggc cgacatcgta accggaccgc gacgccagta agcaccttta
    15001 tagaaaaact gccagatatc ttcgatacgg tgcgcatcgc gaccaataag ctgcggacaa
    15061 aggtgatcct gcaaataaga ggccacggaa agctcacgtc cattgagggt ggcatcccca
    15121 aggcccgtaa taccgtcctc agtggtgatt tttaatgtga cgaaattacg ccccggacag
    15181 gtaacaaaaa cttcagcctt tacgatcttc atgttcgatt ccttgcatcg cttgtcgtga
    15241 tgcatgaaat ctacgcaact gagctactac catacaagta taaagatcaa aaaaagccgg
    15301 agtgatcaca aaaaaaggcg tatatttgcg ccgtgaatgg ttgacaaaag atgaaataga
    15361 atatcttttg tcagctgaca cttccttgta tcttattgat aaaatggatt tatgtcctta
    15421 cgcgcgcccc cagcccgcaa caatgatcaa catgccgcaa agcgcaatca acgcccccgt
    15481 ccagtcataa agactcagtt tcacgccatc cacaacacgc agccaaatca acgccgtgca
    15541 gacataaacg ccaccataag ccgcgtaaac acgcccactc gccgcaggat gcaacgttaa
    15601 caaccagaca aacagtgcca gagaaatccc cgccggaagc aacagccaga tactggcgtt
    15661 tcgttttaac cacaaccagg gcagaaagca tccaataatt tcacacagcg cagtagcaaa
    15721 aaatagtaac gttgttttaa tcatctttgt cacttattga catcatgtat agttatagga
    15781 cgacataata tcatcaatat aaacatcctc ctggtacgtt ttgcgttcgc agtggatggt
    15841 gtagaatcac ctttaatcat tcatacaggg aatgaattat gaaaattact ctcagcaaac
    15901 gaattggcct gctcgctttt ctgctgcctt gcgcaatggc attgagcaca actgttcatg
    15961 ccgaaactaa caaactggtg attgagtctg gcgacagtgc acaaagccgc cagcacgccg
    16021 ctatggaaaa agagcaatgg aatgacacgc gcaatctgcg tcagaaagtg aataaacgca
    16081 ctgaaaaaga gtgggataaa gccgacgccg cttttgataa ccgcgataaa tgtgagcaaa
    16141 gcgccaacat caatgcctac tgggagccaa atactttgcg ctgcctggac cgtcgaactg
    16201 gccgcgttat taccccctaa cctgttattg atttaaggaa tgtaaggaca cgttatgcca
    16261 agcgcccaca gtgttaagct acgcccgctg gagcgtgaag atttacgcta tgtacatcaa
    16321 ctcgacaata acgccagtgt gatgcgttac tggtttgagg aaccctacga agcctttgtt
    16381 gaactctctg atctgtacga taagcatatt cacgatcaga gcgaacggcg ctttgtggtg
    16441 gaatgtgacg gcgaaaaagc cggtctggtg gagctggtgg aaattaacca tgttcatcgc
    16501 cgcgcagaat ttcagataat tatctccccg gaatatcagg ggaaaggtct ggcaacccgt
    16561 gctgcaaaat tagcaatgga ctatggcttc accgttctca atctctataa gctgtatctg
    16621 atcgtcgata aagagaatga aaaagcgatt cacatttacc gcaagcttgg ctttacggtt
    16681 gaaggtgaat tgatgcatga gttctttatt aatggtcaat atcggaatgc cattcgcatg
    16741 tgcatattcc agcatcagta tctggcagag cacaaaacac cgggtcagac tctcctgaag
    16801 ccgaccgcac aatagcatta ataataatcg atagtatttt tgatggtgta aacccgttcg
    16861 acggcgggtt ttactccttc atcaacaatg attagctgac agtccaccgg attggcgtga
    16921 ctgtcatatt cacagctctg ttttacatta ccaactcgtt gattattcag taaagtaacc
    16981 gctgtgtaat ctaatttttt gatcggatcc gttgatggcg tagcgctgac agataatgtt
    17041 ttgtcgttac ttttcgtggt tttacccagc ggataaccct gatcatcata gcgatattcc
    17101 atctgcatct gtttgctgct ggctttaatc acaaagccat tatcatcggt ttcccaactc
    17161 acccctgcag aaggtaattc tgctagctgg cattttccct gcaaacgaac tcttttctcc
    17221 agtgtctggg catcacggta ataattggcg tccagtacca gggcaaccac ggtattattt
    17281 tccagatcca gtaattcgag tgaatcaaaa cagccttctt ccgacaaagt ccccgaaaca
    17341 cgtttcgtca cttcaccttg ctcatccatt aatgtctgag tgaaatcttt taccggacca
    17401 cgcagcggat caaaatcgaa ttcattagag aaactggcca tctcaggggt aaatgaaagt
    17461 gttacctctg tgcggtcaca tcctgtgagg aatatcgcga gtaagcatgg cagtaatttg
    17521 tatttcacaa cagtcaccag agagtagaga tgattctcaa tcatagtagc aaatacagta
    17581 ctttacacgt taaatgctat gcttaaagaa gttatcttcg cgtaaggagc ttatgatgaa
    17641 actctcaacc tgctgtgccg cgcttctgct cgccctcgcc tcacccgcgg tactcgccgc
    17701 gccgggatcc tgtgagcgca tacagagcga tatatcacag cgcattatca ataatggcgt
    17761 tcccgaaagc agcttcacgt taagtattgt acccaatgac caggttgatc agcctgattc
    17821 ccaggtcgtc gggcattgcg ctaatgatac gcataaaatt ctctacaccc gcaccaccag
    17881 cggtaacgtc tctgctcccg cgcagtctac ccaggatggc gcgcctgccg aaccgcagta
    17941 atacattatc gactgcacgc cggatacgac acatccggcg atttgaacat acaacataat
    18001 cccaccttat tactcatacc cttctattga tatggattaa taattcttaa cccaaaatag
    18061 gtagactccc tctattgtta gcgcgctaaa tattcaatat ataaactttt atataacgat
    18121 aaagaacagg gagtgagtta tgtccaaaaa tgaacaaatg gtaggcatca gccgcagaac
    18181 actcgttaaa tctaccgcga taggttctct ggcgctggct gcaggcggtt tttctttacc
    18241 gttcaccctg cgcagtgcag cggcagcggt acaacaggcc agcgagaaag tggtctgggg
    18301 tgcctgttcc gtcaactgtg gtagccgctg tgcacttcgt ctgcatgtta aagataatga
    18361 agtgacctgg gtggaaactg acaacaccgg caacgacgag tatggcaatc atcaagtacg
    18421 tgcctgtttg cgcggtcgct ccatccgtcg gcgtattaat catcccgatc gcttgaatta
    18481 cccaatgaaa cgcgtgggca aacgcggcga aggcaaattc gaacggatta gctgggatga
    18541 agccctggat actatcgcca gtagcctgaa aaaaaccgtc gaacaatatg gcaatgaggc
    18601 tgtatatatt cagtactctt cggggattgt tggcggcaat atgacccgct cttcaccatc
    18661 agcctcggcg gtcaaacgcc tgatgaactg ctacggcggt tcactcaacc agtatggctc
    18721 ctacagcacc gcgcaaattt cctgtgccat gccctacacc tacggcagta atgatggcaa
    18781 cagcaccacg gatattgaaa acagcaagct ggtcgtgatg tttggtaaca acccggcaga
    18841 aacccgcatg agcggcggtg gcatcactta tcttcttgaa aaagcgcgcg agaaatcgaa
    18901 cgcaaaaatg attgttatcg atccgcgata taccgatacg gctgctggtc gtgaagacga
    18961 atggctacct attcgcccgg gcaccgatgc cgcgctggtt gcgggtattg cctgggtatt
    19021 gattaacgaa aatctcgttg atcaaccttt tctcgataaa tactgcgtcg gctatgacga
    19081 aaaaacctta cccgcagatg caccaaaaaa tggtcactat aaagcctata ttcttggtga
    19141 aggagacgac aaaacagcga aaacgccgca gtgggcttcg caaattaccg gtatcccggt
    19201 ggaccgtatc atcaagctgg cgcgtgaaat tggcacagca aaacccgctt atatctgcca
    19261 gggctggggg ccgcaacgcc aggcaaacgg tgagctgacc gcacgcgcta ttgctatgtt
    19321 gcctattttg acgggcaatg tcggcatcag cggcgggaat agtggcgcgc gtgaatcgac
    19381 ctataccatt accatagaac gcctaccggt gctggataat ccggtcaaaa cgtcaatctc
    19441 ctgcttcagc tggacggatg ctatcgatca tggcccgcaa atgacggcaa tccgcgacgg
    19501 cgtccggggc aaagataaac tggatgtgcc cattaagttc atctggaact acgcgggaaa
    19561 taccctggtt aatcagcatt ctgacatcaa caaaacgcat gaaattctgc aggacgaatc
    19621 gaaatgcgaa atgattgtgg tcatcgaaaa ctttatgacc tcatcggcaa aatatgccga
    19681 cattctgctg ccagacctga tgaccgttga gcaggaagat attattccta acgactacgc
    19741 cggtaacatg ggatatctca ttttcctcca gccagtcacc agcgaaaaat tcgaacgcaa
    19801 accgatttac tggatcctga gtgaggtcgc gaaacgtctt ggacctgacg tctatcaaaa
    19861 gttcacagaa ggtcgcacgc aggaacaatg gttacaacat ctgtacgcca aaatgcttgc
    19921 caaagatccg gcgttaccgt cttacgacga actgaaaaaa atgggtatct ataagcgtaa
    19981 agatcccaat ggtcattttg tcgcctacaa agcatttcgt gacgaccccg aggcaaatcc
    20041 acttaaaacg ccttccggta agattgaaat ttattccagc aagctggcgg aaattgcccg
    20101 tacctgggaa ctggaaaaag atgaagtgat aagcccactg ccagtttatg cctcaacctt
    20161 tgaaggctgg gactcccctg aacgtagaac cttcccactg caactgtttg gtttccatta
    20221 caaatcccgt acccactcga cctacggcaa tattgatgtc ctgaaggcag cctgccgtca
    20281 ggaggtgtgg atcaacccta tagatgcaca gaaacgtggg attgccaacg gcgatatggt
    20341 gcgggtgttt aaccatcgtg gcgaagttcg gctaccagcc aaagtgacac cacgcattct
    20401 tcctggagtt agcgctatgg gtcagggagc ctggcacgag gccaatatgt ctggcgataa
    20461 aatcgaccac ggcggctgtg tgaatacgct aaccactctg cgcccttcac cactggcgaa
    20521 gggaaacccg cagcacacta atctggtcga gatcgaaaaa atataaccca ctacgaccat
    20581 aaattttgac atgacatttt gtttgaaaag caataagtga gtaatgatga aaatccatac
    20641 cacagaggcg ctcatgaagg ctgaaatcag ccgtagaagt ctgatgaaaa cctccgcact
    20701 tggcagtctt gcgctggcaa gcagtgcttt cactctgcca ttttcccaaa tggtccgagc
    20761 ggcagagaca ccggtagaag agaaagcggt ctggagttcc tgcaccgtta actgcgggag
    20821 ccgctgtctg ttacgtttgc atgtgaaaga tgacaccgtg tactgggtgg agtctgatac
    20881 gacaggtgac gacgtctacg gtaatcatca ggttcgggca tgtttacgcg ggcgctctat
    20941 ccgccgacgg atgaatcatc ctgacaggtt gaaatatccc atgaagcgcg tcggcaagcg
    21001 cggtgaaggt aaatttgaac ggataagttg ggatgaagcc ctggatacca tcagtgataa
    21061 tcttcggcgg atccttaaag attacggcaa tgaggctgta catgtcctgt acggaacagg
    21121 cgtagatggc ggaaacatca ccaactcgaa cgtcccgtac cgtctgatga actcttgcgg
    21181 tggttttctc agtcgctatg gcagctacag taccgctcaa atcagtgccg caatgagtta
    21241 tatgttcggt gccaatgatg gcaacagccc ggatgatatc gccaatacga aactggtcgt
    21301 tatgttcgga aataacccgg cagaaacccg gatgagcggc ggtggtgtca cttactacgt
    21361 cgagcaagcc cgcgaacgtt caaatgcacg catgatcgtc atcgatccac gttataacga
    21421 cacagctgcc ggacgtgaag atgaatggct gcccattcgc cctggcaccg atggagcact
    21481 ggcctgtgcg attgcctggg tactgattac tgaaaacatg gtcgatcagc catttctcga
    21541 caaatattgt gttggttacg atgaaaaaac gctgcccgcc aacgcaccac gtaacgcgca
    21601 ttataaagcc tatattctgg gcgaagggcc tgacggcata gctaaaacgc cggaatgggc
    21661 agcaaaaatc accagcatac cggcagaaaa aattatccat ttggcacgag agatcggttc
    21721 agcaaaacct gcttatattt gtcagggttg ggggccacaa cgacactcca acggcgaaca
    21781 aacgtcccgc gctattgcca tgctttccgt tctcaccggt aacgtcggca taaacggcgg
    21841 caactcaggt gtacgcgaag gtagctggga tctgggggta gaatggttcc cgatgcttga
    21901 gaatcctgtt aaaacgcaga tttccgtctt tacatggaca gatgccatcg accacggtac
    21961 ggaaatgacc gcgacccgtg atggtgttcg tggaaaagaa aaactggatg tccccatcaa
    22021 gtttttatgg tgctacgcca gtaacacatt gatcaatcaa catggcgaca tcaatcacac
    22081 ccatgaggtg cttcaggatg acagcaagtg cgagatgatt gttggcattg accacttcat
    22141 gacggcctcg gctaagtatt gcgatatcct gttgcccgac ctgatgccaa cagagcaaga
    22201 agaccttatc tctcatgaat ctgcagggaa tatgggctat gtgatcctcg cccaacccgc
    22261 aacctcagca aaatttgaac gcaaacccat ctactggatg ctgagtgaag tcgccaaacg
    22321 cttaggaccg gacgtttatc aaacctttac tgaaggtcgc agtcagcatg aatggatcaa
    22381 atatctccat gcgaaaacga aggaacgtaa ccctgagatg cccgactacg aggagatgaa
    22441 aacgaccggg atctttaaga aaaaatgccc ggaagaacac tacgtcgctt tccgcgcatt
    22501 ccgtgaagat ccacaggcaa acccgttgaa aacaccttcg gggaaaatcg aaatttattc
    22561 tgaacgactg gcgacgattg cggatacctg ggaattgaaa aaagatgaaa ttattcatcc
    22621 ccttcctgcg tataccccag gttttgatgg ctgggacgat cccctgcgga aaacctatcc
    22681 actgcagcta acgggcttcc actacaaagc gcgtacccac tccagctacg gcaatattga
    22741 tgtgttacag caggcctgcc cacaagaggt gtggatcaac cccattgatg ctcaggcacg
    22801 cggtatccgt catggcgata ccgtgcgggt atttaacaat aatggagaaa tgctgattgc
    22861 cgcaaaagtg actccgcgta ttctgcctgg cgttaccgcc atcgggcaag gtgcgtggct
    22921 taaagcggat atgtttggtg accgggtcga tcacggcggc agtatcaata ttctgacctc
    22981 tcaccgccct tcaccgctgg caaagggaaa cccgtcgcac agcaatcttg tccagatcga
    23041 aaaggtttaa ggagtagccc atgaccacac aatatggatt ttttatcgat tccagccgct
    23101 gtactggctg taaaacctgc gaactggcgt gcaaagattt taaagatctt ggcccggaag
    23161 tcagtttccg ccgtatttat gaatatgctg gcggcgactg gcaggaagat aatggcgtct
    23221 ggcaccagaa cgtgttcgct tattatctct ccatctcctg taaccattgc gatgaccccg
    23281 catgtacaaa agtctgcccc agcggcgcaa tgcataagcg tgaagatggc tttgtggtgg
    23341 ttgatgaaga tgtttgtatc ggctgccgct actgccacat ggcttgtccg tacggcgcgc
    23401 cacagtacaa tgctgaaaaa gggcacatga cgaagtgcga tggttgttat tcgcgcgtcg
    23461 ccgaggggaa acaacccata tgtgtcgaat cctgcccgct gcgtgcactt gagttcggtc
    23521 caattgaaga gctgcgccag aagcacggca ctctggctgc cgtcgcgcca ctgccgcgcg
    23581 cgcatttcac aaaacccaat atcgttatca aacctaacgc caacagccgc ccgaccggcg
    23641 ataccactgg ttatctggct aatccggagg aagtgtaaaa tgggaaatgg atggcatgaa
    23701 tggccactgg ttatctttac cgtattgggt caatgcgtag taggcgcgct gattgttagc
    23761 ggaattggct ggttcgccgc caaaaatgac gcagaccgtc agcacattgt tcgtggcatg
    23821 tttttcctct ggctgttgat gggcatcggg tttattgcct ctgtcatgca cctgggatcg
    23881 ccactgcggg cgtttaactc gctgaatcga atcggagctt ctggtctgag taacgagatt
    23941 gctgccggtt caatattttt tgccgttggc ggattatggt ggctggtgac gatcatcggt
    24001 aaaatgccgc aagcgttggg gaaactctgg ttacttgtca gcatggcgct cggcgtcatt
    24061 ttcgtctgga tgatgaccgg cgtgtatcaa atcgacactg tgccaacctg gcataacggt
    24121 tacactacgc tggctttctt tctgacagta ttattgagcg gtccaatact tgctgctgca
    24181 attctgcggg cagcccgcgt tacatttaat accacaccat ttgccatcat tagcgttctg
    24241 gcgctgatcg cttgtgccgg ggtgattgtg ctgcaaggtt tgtctctggc gtctattcac
    24301 tcatccgtac aacaagccag cgcactggta ccagattacg cctccttaca ggtctggcgt
    24361 gtggtattgt tatgcacagg tctaggatgc tggctttgtc cgctcattcg tcgccgtgaa
    24421 cctcacgttg ccggacttgt tctggggcta atcctcatac ttggcggtga gatgatcggg
    24481 cgtgtgctct tttatggcct gcacatgacg gtaggaatgg ctattgcagg ttaacaaatt
    24541 ggtgcggggc aacccgcaca tttcaggatg ttaggaatga cccatttttc acagcaagat
    24601 aatttttctg tcgcggcgcg cgtgttgggc gcgctgtttt attacgctcc agagagtgcg
    24661 gaagccgccc ccctggttgc ggtactcacc cgtgacggct gggaaactca gtggcctcta
    24721 ccagaagcgt cattagcgcc gctggtgacg gcttttcaga cacagagtga agagactcac
    24781 gcccaggcct ggcagcgttt gtttgtcggc ccgtgggcac tgccgtcacc gccgtggggg
    24841 tcggtctggc tggatcgcga atctgtgctg tttggcgatt caacattggc acttcgtcag
    24901 tggatgcgcg agaaaggcat tcagtttgaa atgcagcaaa acgaaccgga agatcatttt
    24961 ggttcactgc tgttgatggc tgcgtggttg gcggagaatg gtcgccagac agaatgcgaa
    25021 gaattgctgg catggcacct ttttccgtgg tcaacacgat ttcttgacgt ttttatcgaa
    25081 aaagcagaac accctttcta ccgtgcactg ggtgaactgg ctcgcttaac gctggcgcaa
    25141 tggcaatcac aactgttaat tcctgtcgcg gttaaatcgc tgtttcgata ggatcaccgt
    25201 aatattgccg gatggtgacg attacgcgca attcggcaat aatacgttac agtgtcacgt
    25261 tttttatctc ttaaagcacg cactgctttt gcgtctggct tcttttgccg caaaatagtc
    25321 gcccgtgttt cattgcccat ttctgctcat gcatcatcta cacatctatc cggatctgcg
    25381 cactatgttc cgccgtctgc ttatcgcaac agtcgtcggt attctcgcgg cctttgccgt
    25441 tgccgggttt cgtcatgcaa tgctgctact ggagtggttg ttcctcaata atgactcggg
    25501 cagtctggtc aatgcagcga caaatctttc cccgtggcga cggctgctaa ctccggcgct
    25561 cggcggactg gcggcgggtt tgttgctgat gggctggcag aaatttaccc agcaacgccc
    25621 tcatgcgccg accgattaca tggaagcgtt gcaaaccgat gggcagtttg attacgcagc
    25681 aagcctggtt aaatcgcttg cctctctgct ggtagtaacc agcggcagtg caattggtcg
    25741 cgaaggtgcg atgattcttt tagctgccct tgccgcctcc tgttttgtcc aacgttttac
    25801 gccacgccag gagtggaaat tatggatcgc ctgtggagct gcggcgggaa tggctgcggc
    25861 ctatcgcgcc ccgcttgctg gcagtttatt tatagccgaa gtgctgtttg gcactatgat
    25921 gttggcctct ctcggcccgg tgattatttc cgccgtcgtg gcgttgctgg ttagcaatct
    25981 gattaatcat agcgacgcgt tactctacag cgtacaactc tcagtgacgg ttcaggctcg
    26041 tgactatgcg ctgattatca gtacaggtgt gctggcaggt ctgtgcggac cactgttgtt
    26101 aacgttaatg aacgcctgtc atcgtggatt tgtaagtctc aaacttgcgc cgccctggca
    26161 actggcactg ggcgggttga tcgtgggtct gctttccctg ttcacacctg ctgtgtgggg
    26221 caacggctat agcaccgtac aatccttttt aaccgccccg ccactgttaa tgatcattgc
    26281 cgggatcttc ctctgtaaac tgtttgccgt gctggcgagt agtggttctg gcgcgcccgg
    26341 tggtgtcttt acaccgacgc tatttatcgg tcttgccatt ggcatgttgt atggtcgtag
    26401 cctgggatta tggttccctg atggcgaaga aattacgctt ttactcggat tgaccgggat
    26461 ggcgacactg ttggcggcaa ccacgcacgc gccgattatg tcgacgttga tgatatgtga
    26521 aatgaccggg gagtatcagc tactccccgg tttattgatt gcctgcgtaa ttgcgtcggt
    26581 aatttcgcgg actttacacc gtgactctat ctaccgccag cacactgcgc agcatagcta
    26641 agcggatgta ttgccccagt tcacgctgtt cggcgcgcgg cagataaggc agttcaccaa
    26701 tgagcggtgc cggaagtttt ttacccagca catcaatgat ttccgcataa tgcgccagtc
    26761 ctggattgat tcggttagcc acccagccaa tgagcggtag cccgtcgttg gcgatcgcct
    26821 gagctgttag cagtgcatgg ttaatgcaac cttcctgaat accgacaacc atcaacaccg
    26881 gcagttgttc ctgcaccacc cattcagaga gcggacgcaa atcattcatc agactgcgcc
    26941 agccgccagt cccttctacc accacatgat cgactttatc ggtcaggttt gccaggccgt
    27001 ttgaaatgag ggtgtaattg attgggcaac tgtgcgccac gctactttct tcttcgctta
    27061 acgcgatagg attaactgct tcataaggca gttcgatggt tgaaacactc tgcaacacca
    27121 gggcatcttt attacgcagc ccttcgggtg tctctttgct ccccttcgct acgggtttat
    27181 atcccgcaac cgtttttccc tgggaggcta acgcttgtag caatgcgcgg gaaaccaccg
    27241 ttttccctac agaagtgtct gtaccggtaa taaagaaacg cttcagcatc actaactcca
    27301 ccgttatgct tcacaaatat aaaccaggaa aataattaac cttgaaagtc taagttatgc
    27361 tttcctggcc caaattgaga tagcgcaaat tttggtagaa cagttaaaaa atgttaaccc
    27421 tgcaacagac gaatcaacaa agaaccgtta tacatcgcgt cttttaccag cgcagcccct
    27481 gccatcgtgc cctggttaga aaactgagta ctctcaacgc taatgtgctg actatacgca
    27541 ggaagggcct gctgacggat gctatctgag atgaccggga agaggatatc tgccgcttta
    27601 cttaacggtg agccaatcag tattttttgt gggttaaata aattcaccat gatggcaaga
    27661 atgcgcccta catgcgcgcc caccccggta atgatgtctt ttgccagcag atcgccgcgc
    27721 aatgccgcct gacacaatga gtccacggtt aacggttgtc catgtaacat cgagctcatg
    27781 gattgattga gacgcagctg tgccagctca agaatactgt ccacactggc gatggtttcg
    27841 aggcagccgt gattcccgca ataacagcgt ttcccatacg ggtcgacctg cgtgtgacct
    27901 atttccacga ggctactgct gcctgcgtgt agcagatgac catcggtaat gacgcccgcc
    27961 cccacgttgt gatcgataac cacctgaatc acatcgcgcg ccccgcgtga ggcaccaaac
    28021 aaggcctcag ccatcgtcca tgcgctgata tcatgctgaa tataaaccgg tacgccggta
    28081 tgctgctcca gcgcctcgcc gagcggcatc tcttttacat cctcgtagaa cggcatgcga
    28141 tgtacaatac cattttccgt atcaataatt cccggcaagg taatggcaat cgaagtcaga
    28201 cgctcaagtt ttttctggtg gcggataaaa aactgatcga tatgggaaat gatacgatcc
    28261 agcaatggtg agtcatcttt taacgccagt tcctgcgcct cctccaccac cagtttgctg
    28321 ctcagatcgc gcagagcaag gaaaatctcc ccgcgactaa tgcgcagaga aagatagtgc
    28381 caggcttcag tttcaaccac cagccccact gccggacggc cacggttccc cgcttctttg
    28441 atttccagct cttgcaccag gtgtgcttcg agcatctcac ggacaatttt agtgatactg
    28501 gcaggagcca gctgcgccag acgggaaaga tcgatacgcg agactggacc aagctgatca
    28561 atcaggcgat aaaccgcgcc cgcgttggtc tgctttattt gatcaatgtg cccaggctga
    28621 ttttcagcaa ccaccgcata ctccctatat tttcgcgctc cgaaataatc tgtaggctat
    28681 ggtgaagcac ttcaatacgt gtcgtcaaat ttttacttag gcatgtgatt aacagcacat
    28741 ttttcgggct ttttcgctga aatttccctc acctgagagc attcagcaga tgaatacgaa
    28801 agttacgcgc agccggactt tgttcatgat gtttcggcca gaccaaccac atttcagaaa
    28861 ccgcatcctc ttcagcaatc ggcacccagc gcatttcgtt gagctgaaca cgtttaaatg
    28921 acgcaggcaa aattgaaaca cccagaccgg cggaaaccag accgatgatg gtcattgcct
    28981 cacccacctc ctgagtgatg acgggcgtca aattgtaacg tctcatcagc ccgagaatat
    29041 cgtcatacag ccctgtcccg acgtgcggat caaaaaagac aaagggttct ttcgccagtt
    29101 cagccagcgt tacactcggg ttatttgcca ggggatgatc gtgcgggatc atcgccataa
    29161 gcggttcatg gacgatgact gcgtgttcaa gcgtctccgg taacgctgtg ttacgcagca
    29221 atcccatatc cagcgttcct tcaatgagcg gagcgatttg ctcgcgagtg ttcatttcgc
    29281 gggtttgtaa atggacatcg ggataatcac ggcgaaacag cgataacgta tcggacaccg
    29341 cccgaataaa aggagcggac gaagtaaaac caatgcgcaa ctcccccgct tcaccctgat
    29401 gcagtctttc agcgcgagcg gcagcatcat ccaccataga caggatttgc cgactatcta
    29461 caagaaactg ttttcctgct gccgtcagca atacactgcg attggtccgt gccagcagtc
    29521 gggcaccaat ttgttgctcc agcgcctgaa tctgttgact tagcggtggt tgcgaaatat
    29581 tcaggcgggc agcggcgcgc ccgaaatgca gctcttctgc aaccgcaaca aagtaacgca
    29641 gatgacgaag ttcaatattc atatttaaaa catcttattt gagattatta atatattaga
    29701 cagaacaatt cgattttcct aatctattca taagcctgat ccacaggcat atttagcaag
    29761 gatttcaagt gagccgtact acaactgttg atggcgctcc ggcaagcgac actgacaagc
    29821 aaagcatttc tcagccaaat caatttatta aacgcggtac gccgcaattt atgcgtgtca
    29881 ccctggcgct tttctctgcc ggactggcaa ccttcgcact tctctattgt gtgcaaccta
    29941 tccttccggt gctttcgcag gagtttggct taacgcctgc taacagtagt atttcactgt
    30001 ccatttcaac ggcgatgttg gctatcggtt tgctgtttac tggcccgcta tccgatgcca
    30061 ttggtcgcaa accagtgatg gtcacggcgc tactgttggc ctccatttgt acgttacttt
    30121 cgacaatgat gaccagctgg cacggcattt tgattatgcg cgccttgatt gggctttcgt
    30181 taagtggcgt ggcagctgtt ggcatgactt atcttagcga ggaaatccac cccagtttcg
    30241 tggccttttc gatggggttg tatatcagcg gcaactcaat tggcggtatg agcggacgct
    30301 taattagcgg tgtcttcacg gactttttca actggcgaat tgctctggcg gcaatcggtt
    30361 gtttcgcgct ggcctcggcg ttaatgttct ggaaaatcct ccctgaatca cgccattttc
    30421 gcccgacttc gctgcgacct aagacgttgt ttatcaactt tcgtctgcac tggcgtgacc
    30481 ggggattacc gttattgttc gcagaaggct ttttgctgat ggggtcgttc gtcacgctgt
    30541 ttaattacat cggctatcgg ttgatgctct ctccctggca tgtcagccag gccgtggttg
    30601 gcttattatc gctggcttat ttgaccggta catggagctc acccaaagcc ggaaccatga
    30661 ccacccgcta tgggcgcggt ccggtgatgt tgttttcgac gggggttatg ctgtttggtt
    30721 tactgatgac cttatttagc tcgctgtggc tgatctttgc cggaatgtta ctcttctcag
    30781 ccggattctt cgcagcccac tcagtagcca gcagctggat cggcccccgc gcaaaacgcg
    30841 ctaaaggcca ggcctcctcg ctgtatctgt tcagttacta tctggggtcg agtattgccg
    30901 ggacgctggg tggtgttttc tggcataact atggctggaa cggcgtcggc gcatttattg
    30961 ctctgatgct ggtcattgct ctgctggtcg ggacgcgttt gcatcgtcgt ctgcacgcct
    31021 gaaaaataag tccggactgc ggtaaatacc catccggact tattgcctgc tcaaaccaac
    31081 gttaatagcc atcctaaaat agacgaagcg ccagccaatt cccgcagcgc atctagtgtc
    31141 atcaggatta taagtaccca aataaacgga ttcattttgc tgtgtgtcat ttattactga
    31201 tgcgcagtta ttctaccgct ttgtaagtag taaaatagtt aacccgatca agactactat
    31261 tattggtagc taaatttccc ttaagtcaca atacgttatt atcaacgctg taatttattc
    31321 agcgtttgta catatcgtta cacgctgaaa ccaaccactc acggaagtct gccattccca
    31381 gggatatagt tatttcaacg gccccgcagt ggggttaaat gaaaaaacaa attgagggta
    31441 tgacaatgaa aaaagtatta gctctggttg ttgccgctgc tatgggtctg tcttctgccg
    31501 cctttgctgc agagactgcg accacacctg ctccgactgc gacgaccacc aaagcagcgc
    31561 ctgcgaaaac cacacatcat aaaaaacagc ataaagcagc acctgcccag aaagcgcagg
    31621 cggctaaaaa gcatcataaa aatgccaaag ctgaacagaa agctcctgaa caaaaagcgc
    31681 aggcagcgaa gaaacacgcc aagaaacaca gccatcagca accggcaaaa cctgctgcac
    31741 aacccgcagc gtaagttatc aatagtaatg ctggcgcgcc ccctcgcgcc tgaaaattac
    31801 ggtgctaagc ggataacgtt tagcaccgcc tttagcggag ggtagtatgg tgggccgtta
    31861 tcgctttgag ttcattctta tcatccttat tttatgcgca ctgattgccg cccgttttta
    31921 tctttcctga tcgtagttat ctgattttac tcccactttc atcccgtccc gtctatagta
    31981 tttatgaggg tttgctttta ataatcataa ttacccacca gagtgtgata tgcgtacaac
    32041 cattgctgta gtgttgggtg caattagttt gacgtctgct tttgtgtttg cagataaacc
    32101 agacgttgcc aaatcggcaa acgatgaggt cagcaccctg ttttttggtc atgatgatcg
    32161 tgtgccagtg aatgacacga cccaatcacc gtgggatgcg gttggccaac tggaaacggc
    32221 cagcggcaat ttatgtacgg cgacgctgat tgcacccaat ctggcattaa cggcaggaca
    32281 ctgtttattg acgccgccaa agggtaaagc cgataaagcg gtagcgctgc gtttcgtgtc
    32341 aaataaaggt ctttggcgct atgagatcca cgacatagaa ggccgcgttg atccgacact
    32401 gggaaagcgg ttaaaagcag atggggatgg ttggattgtt cctcccgcag ctgcaccgtg
    32461 ggacttcggt ttgattgtgc tacgtaatcc gccttctggc attacaccat tgccgttatt
    32521 tgagggagat aaagccgcgc ttaccgccgc attaaaagcg gcaggtcgta aagtgactca
    32581 ggcgggatac cctgaagatc atctcgatac gttgtacagt catcaaaact gtgaagtgac
    32641 tggctgggcg caaacgtcgg tgatgtcgca tcagtgcgat accttgccgg gtgacagcgg
    32701 ttcgcctctg atgttgcata ccgatgacgg ctggcaatta attggggtgc aaagttcggc
    32761 tcctgccgcg aaagatcgct ggcgcgccga taaccgggcc atttctgtta ccggttttcg
    32821 cgacaggctg gatcaactgt cgcaaaaata atgttcaaac gctgcccgac agcgcgggca
    32881 gcgtgttcat caggcaagtt tcaccatgat cattccagcc aacagcaaga ccaggccaat
    32941 ccagccttta cgatttaacc gttgaccaaa caagatccaa cctgcggcta acgtggcggc
    33001 aataccaaac ccgccccaca atgcataagc gacagacaag tcgatccctt taacggcctg
    33061 agaaagcgca ctaaaggcag ccagcaccgc cgccagggag agcaagccaa atattttgcg
    33121 acgaaagccg tcagaaaatt tcaaaaagac gttagcaacg atttccagca cgattgccaa
    33181 tgccagccag gcggcgtgaa cccattcaaa ctgcgccatg gttcacctcc agttcaggtt
    33241 tacgcgcttt acgggtacct gatttgatca atacaatccc ggccaccagg gtggttaacc
    33301 cggcaatttt catcagcgat aaactttcgt cgaataacaa aacactaaac aaggtaataa
    33361 ataaaatacc gataccttcc cacagcgcat aagccacgcc taaggcgatc tttttaacgg
    33421 cgaaagagag aaatatatac gacagagaaa tcatcaccag cattaaaata aagccgccat
    33481 ttccctcact gacgctcgcc catttcatag acagcgtacc ggtaatttct gtagcaatag
    33541 ccagacctaa taaaatccaa taaatataca ttgtccttct cctgcaagag aattatttta
    33601 attttcgctt aattcagcga aaccaaagta aagtggtcaa gccccgcgca tagcgtaagc
    33661 ttaagcagaa gaaaggacta aagcgcgttg cgccagtgct gctcacctac gagcaagata
    33721 gtggatgagg tacgaagatg ggtaaatgta gaaaataacg tcctgaacaa attgtccata
    33781 atattacaat tatccgcagt gttgcttctc gtcatcgcgg atgataattg tcctcggtag
    33841 ttgaacacgc ctgatttgta tcatagctta agaattaact caaaatattt tcacttcttt
    33901 acctgagcgg tttgattttc gttatgatga cggagcgaaa aagacattat tattagcaaa
    33961 ggaagaaaaa acggggacaa gcatggcaaa gccgatcatc acgctcaatg gcctaaaaat
    34021 cgtcattatg ttgggaatgc tggtcattat tctctgcggt atccgttttg ccgccgagat
    34081 catcgtgccg tttattctcg cattatttat tgctgttatt cttaacccgc tggtgcaaca
    34141 catggtccgc tggcgagtgc cgcgtgtact ggcggtgtcg attttgatga ccatcatcgt
    34201 gatggcgatg gtgttgctgt tagcttatct gggttccgca ctcaacgagt tgacgcggac
    34261 gttaccgcaa tatcgcaact ctattatgac gccgctgcaa gcgattgaac cgttgttgca
    34321 acgcgtaggg attgatgtct cagttgacca actggcgcat tacattgatc cgaacgcggc
    34381 gatgacgttg ctcaccaact tattgacgca gttatctaat gccatgtcat caatattttt
    34441 attgctgctg acggtgctgt ttatgctgct cgaagtgcca caattgcccg gaaaatttca
    34501 gcaaatgatg gcgcgtccgg ttgaagggat ggcggcgatt caacgtgcga ttgacagtgt
    34561 ttctcattat ctggtgctga aaacagccat cagcatcatc accggcctgg tcgcctgggc
    34621 gatgctcgcc gcactcgatg ttcgcttcgc ttttgtctgg ggattgctgg cctttgcgct
    34681 taattacatc cctaatattg gttcagttct cgcggcaatt ccccctatcg ctcaggtact
    34741 ggtgtttaat ggcttctacg aagcgttgct ggtgctggcg ggatatctgc tgattaatct
    34801 ggtcttcggc aatattctgg agccacgcat catggggcgt gggctggggc tttccacatt
    34861 ggtggtattt ttgtcgttga ttttttgggg atggttgtta ggaccggtgg gtatgctgct
    34921 ttccgtgccg ttgacaatta ttgtcaaaat tgcgcttgaa caaacagcgg gaggtcaaag
    34981 catcgccgtt ctgttaagcg atctcaataa agagtgacgg cctccgcaga ggccgtcagg
    35041 gttacagagc tttcaggatt gcatccacgc tggctttggc gtcaccaaac agcatgtggg
    35101 tgttttcctt gaagaacagc gggttttgca caccagcata gccagtgttc atcgaacgtt
    35161 taaagacaat cacgttctgc gctttccaca cttccagcac aggcatacca gcaatcggac
    35221 tcttcggatc atcctgcgcc gccgggttaa ccgtatcgtt agcaccaatc accagtacgg
    35281 tatcggtatc agcaaagtca tcgttgatct cgtccatttc cagcacgatg tcatacggta
    35341 cttttgcttc agccagcaat acgttcatat gtccaggcaa acgccccgca accgggtgga
    35401 taccgaaacg cacgttgata ccacgagcgc gcagtttctc ggtaatttca gcgacaggat
    35461 attgcgcctg cgcgactgcc atgccgtacc ccggagtaat gatcactgaa tgggagtttt
    35521 tcagcagttc cgctgtctct tctgcggtga tttcgcggtg ctcgcccact tcctgatcat
    35581 cgccagtaga agagccgtcg gtgccgaaac cacccgcaat aacgctgata aaggaacggt
    35641 tcatcgcctt acacataatg taagaaagga tagcgcccga agaaccgacc agcgcaccgg
    35701 tcacaatcag caggtcgttg ctgagcataa agcccgcagc cgcagccgcc cagccggagt
    35761 acgagttcag catcgacacc accactggca tatctgcacc accgatggag gcgactaaat
    35821 gccagccgaa taccagcgca atggaggtca ttatcagcaa tgccagcact tgcaggccga
    35881 cgctgtccgt gcgaacaaat acaatcagca gcaggaagga aacgaccaga gccgccaggt
    35941 tcattttgtg acggtttggc agcatcaacg gtttagacga aatcttgcca cacagtttgc
    36001 cgaacgccac caccgacccc gtgaacgtta ccgccccgat gaagataccg aggaacactt
    36061 ccgtcaggtg aatattgacc agaatcggtg ccattcccgc gtcatgatgc agatagctgt
    36121 taaagccaac cagcactgcc gccagaccca cgaagctatg caggatcgcc accagttctg
    36181 gcatttcggt catttcaact ttcttcgcca gccggatacc aattgcccca ccaatgacca
    36241 tcgccagcaa gatccagcca acattacccg tatccggtcc aaaaatggtt gcgattaacg
    36301 caatcgccat cccggcgata ccgaagttgt taccctggcg agacgtttca tgtttcgaaa
    36361 gcccggccag actgaagata aacaggatcg cggcaacaat gtatgcagct gtaactaatc
    36421 ctccagacat atgttacccc ttaatttttg cggaacattt tcagcatgcg ctgagtcacg
    36481 gtgaagccac cgaaaatatt aatgctggct ataagcaccg cgataaaact aaggaagcta
    36541 acccagccgc cctggccaat ctgcaacagt gctccgacaa caataatccc tgaaatcgcg
    36601 ttggtgaccg acatcaacgg tgtatgcagc gcgtgcgaaa cattccacac cacgtaataa
    36661 ccgacaacgc aggccagcgc gaacacagtg aagtgcccaa ggaactcttt cggcgcaacg
    36721 cttgccaacc agccgaaaag aatgattgcc agcgccatca acgcgtattt acgccacggt
    36781 gagcaggcac atttttcctc agttttcact tccggtgccg ctttttgtgc cgcctgcggc
    36841 tgagctgata cctgaatcgg cggtgccggc caggtaattt cgcccgcacg gatcacggtc
    36901 acgccgcgaa tcaccacatc atcaaaatca acagtgatat tgccgtcttt ctctttgcac
    36961 aacagtttca gcagattaac gaggttagta ccgtaaagct gtgaggattg cgtcggcaga
    37021 cggcccggaa gatcggtgta accaatcact ttgacaccat tttccgtagt gaagatttca
    37081 cccggcacgg tgtattcaca gttaccgccg ttctgggctg ccaggtcgac aatcacactg
    37141 cccgccttca tggagtcaac catttcacgg gtaattagct tcggcgctgg tttgcctgga
    37201 ataagcgcgg tggtgacaat gatatcgacc tcttttgcct gggcggcaaa gagttccatt
    37261 tccgctttga tgaacgcgtc cgacatcact ttggcatagc catcgccgct gcccgcttcc
    37321 tctttaaaat ccagctcgag gaattccgcg cccatacttt gaacttgttc tttcacttcc
    37381 gggcgggtgt cgaatgcacg cacaatcgcg ccgagactgt ttgctgcgcc aatggcggcc
    37441 agacctgcaa cacccgcacc aatcaccatc acttttgccg gtggcacttt cccggccgca
    37501 gtaatttgcc cggtaaagaa gcgcccaaat tcatgtgccg cttcaacaat ggcgcgataa
    37561 ccggcgatgt tcgccatcga gcttagtgcg tccagcgatt gtgcgcgtga gatacgcggc
    37621 acagagtcca tcgccatcac ggtcacgtta cgttccgcaa gtttttgcat taattccgga
    37681 ttctgcgcag gccagataaa actcaccagc gttgtcccag gattcagtaa cgcaatttca
    37741 tcatctaacg gcgcattgac cttcagaatg atctctgact gccagacgct attcccttct
    37801 acaatttcag cgcccgcttg cacaaacgct ttatcgtcaa aacttgccag ttgacccgcg
    37861 ccgctctcta ccgcgacggt aaaacccagt ttcagcagct gttccactgt ttttggcgtt
    37921 gctgcaacac gggtttcatt ggttaaccgt tctcttggta tgccaattcg catgatattc
    37981 ccttccatcg gttttattga tgatggtttg cctgtgtcag gagtcacaca agccgctcat
    38041 gtacgagcta aatgttactc cgttaaaata aattagtaac aaacgcccta taacgtactg
    38101 aaaattatgc ctgtgatcta gcgccaaaaa tcagtatttc ggcgtgaact cgcaaaatat
    38161 taacgattca gccgtgatag tgggataaac accttagaac gccggataaa gactgataat
    38221 tgtcttcggc ggtcgggtaa aacgagacat cgccccggca cgaatcacta cttaacatta
    38281 aattaactta tacaattcag ttgcttcagt agtaatgatg ctgatacggc tgttttttaa
    38341 gcatagacgg tcatttgagc aggattaaaa ttggcttaag gaatgtgata tgaaaaatga
    38401 cgcagacagt tacaccgttt aaatgcaata atcagccacg tttctcgtta ataacaatac
    38461 cagtacctgg tttgcgcaag gcgaaggatt atttttatga agcttaagaa caccctcctg
    38521 gcgtcggcac tgctttctgc tacggcattc tccgttaacg cagcaacaga actgacaccg
    38581 gagcaagcgg cagcggttaa accttttgac cgtgtagtgg ttaccggtcg ttttaatgct
    38641 attggcgaag cggtgaaagc cgtttctcgt cgcgcagata aagaaggtgc cgcctctttt
    38701 tatgttgtcg acacttctga ttttggtaac agcggtaact ggcgtgtggt cgctgacctc
    38761 tataaagccg atgctgaaaa agcagaagaa acaagtaatc gcgtaattaa cggtgttgtc
    38821 gaactgccga aagatcaggc tgttctgatt gaaccgtttg acacggtcac cgtccagggc
    38881 ttctatcgta gccagccaga agtcaatgat gccatcacca aagcggcaaa agcgaaaggt
    38941 gcctactctt tctacatcgt tcgtcaaatc gatgccaacc agggcggcaa ccagcgtatt
    39001 actgcattca tctataaaaa agatgcgaag aaacgtatcg tccagagccc ggatgtgatc
    39061 ccggcagatt ccgaagcagg acgtgcagct ctggctgccg gtggcgaagc tgcgaagaaa
    39121 gttgagatcc cgggtgttgc gactaccgca tcaccaagtt ctgaagtcgg tcgcttcttt
    39181 gaaacccagt catcaaaagg cgggcgttac accgtcacgc tcccggatgg cactaaagtc
    39241 gaagaactga acaaagcgac cgcagcgatg atggtcccgt tcgacagcat caaattctct
    39301 ggcaactacg gcaacatgac cgaagtctcc tatcaggttg cgaaacgtgc cgcgaagaaa
    39361 ggtgctaagt attaccacat cacccgccag tggcaggaac gtggtaataa cctgaccgtc
    39421 agcgcagatc tgtataaata actattcagt tactcctata aggcggcttg atgccgcctt
    39481 tcgcattttt agtcacaaat ttccccgcga ccattgcatg cagacacatc tcttcgtaaa
    39541 atcccgcgct tcagtggcaa actccatttt tatgcgtttt gccctaatat ttattcttct
    39601 tgtcacgttt taatcactgg atatcgatgg aaaagaaact gggactgagc gcactcaccg
    39661 cgctggtatt aagctcaatg ctgggcgcgg gtgttttcag tctgccgcaa aatatggcgg
    39721 cagttgccag cccggcagca ctgctcatcg gctggggtat tactggcgct ggcattttat
    39781 tgctggcctt tgccatgctg atcctcacgc gcattcgtcc cgaacttgac ggcggtatct
    39841 ttacctatgc ccgcgaaggg tttggcgagc taatcggctt ttgttccgca tggggatact
    39901 ggctgtgcgc agtcatcgcc aacgtctcct atctggtgat cgttttttcc gcgttaagct
    39961 tttttacgga cacgccggaa ttgcgcctgt ttggtgatgg taatacctgg caatcgatcg
    40021 tcggtgcatc ggcgttattg tggattgttc actttttgat cctgcgcgga gtgcaaaccg
    40081 ctgccagcat taacctggtg gcgacactgg caaagttgtt gccgctgggt ctgtttgttg
    40141 tgctggcgat gatgatgttc aaactggata ccttcaagct cgacttcacc ggacttgcac
    40201 ttggcgtacc cgtgtgggaa caggtgaaaa acaccatgct gatcaccctg tgggttttca
    40261 ttggtgttga aggtgccgtg gttgtttctg cgcgtgcgcg taataaacgt gatgttggca
    40321 aagcgacact gctggcggtt ctctccgctc tgggcgttta tttgctggta acgctgcttt
    40381 cgctgggcgt ggtggcacgt cctgaactgg ctgaaattcg taacccgtca atggccggtc
    40441 tgatggtgga aatgatggga ccgtggggcg aaatcatcat cgctgccggt ttgatcgttt
    40501 ccgtttgcgg cgcgtactta agctggacca tcatggcggc agaagttccg ttcctggctg
    40561 ccactcataa agcattccca cgcatttttg cgcgccagaa cgcacaagcc gcgccatccg
    40621 cctcactgtg gttgaccaac atctgcgtgc aaatttgtct ggtgctcatc tggctgaccg
    40681 gttccgatta caacacgttg ctgaccatcg cctcagaaat gattctggtg ccctatttcc
    40741 tggtcggcgc gttcttgctg aaaatcgcaa cccgtccact gcataaagcg gtaggtgtcg
    40801 gtgcctgcat ttatggctta tggttattgt acgcctctgg tccaatgcac ttgctgttat
    40861 ccgttgttct gtatgcgccg gggctgctgg ttttcctgta tgcgcgtaaa acgcatacac
    40921 atgataacgt actgaaccgt caggaaatgg tgctaattgg catgctgtta attgcctcag
    40981 ttccggcgac ctggatgctg gtgggataac gttcatccca tcgttggata gcaaaggaga
    41041 taacgatggg taaagcccag cccttgccaa tattaattac tggcggaggt cgtcgcatcg
    41101 gcctcgcact cgcctggcat ttcattaatc aaaagcaacc ggtgattgtc agctatcgga
    41161 cgcactatcc agccattgat ggactgatta aggcaggtgc gcagtgtatt caggctgatt
    41221 tttcgaccaa cgacggtgtg atggcgtttg ccgatgaagt actaaaaagc acccatggcc
    41281 tgcgtgctat tttgcataac gccagcgcgt ggatggcgga aaaaccggga gcgccactga
    41341 ccgacgtact ggcttgcatg atgcagatcc acgttaatac cccatatcta ctcaaccatg
    41401 ccctggaaag attactgcgt gggcacggac acgccgccag cgatatcatt cactttaccg
    41461 attatgtggt ggagcgcggt agcgacaaac atattgccta tgctgcaagc aaagcggcac
    41521 tggataatat gacccgttcg tttgcccgca agctggcacc ggaagtgaaa gtgaattcta
    41581 ttgcgccatc gctgatcctg tttaatgaac atgatgatgc cgaatatcga caacaggcgc
    41641 tgaataaatc actgatgaaa accgcgcctg gcgagaaaga agtgatcgac ctggtcgatt
    41701 acttacttac cagctgcttt gtcaccggac gcagtttccc tcttgatggc ggtcgtcatc
    41761 tgcgttaatg cagcttaatc cagcatataa tcaacaccca tgcgcttatc ccccagcatg
    41821 caacagaacc aacaaaagcg gcgggcaagc gcatcattcc ggtgaaatac cacagcgacg
    41881 ccaggtaaac aaaataggga ataatcgacc acatgctaaa aatgatggtt gcgcgtaagg
    41941 cttcaatgcc gcgttcgctg gcaacaatat aatgcgcgat gagcgcaaag gtcgggaaaa
    42001 gtgggatcag cccggcgata taataatttt tcgtttttgc taaaacacca atcaacagca
    42061 ctaccagcgc accgagcgcg gctttgatta ccagccccat ctttttacct taacacttcc
    42121 ataacaagtc atcagtagaa tacctgatga aaacttgttt agaaacgatt gatagtaagt
    42181 aaaaacagcg cggtgtattg tgacgttttt atatctaccg tgaataatat gaacactatc
    42241 gtatttgtgg aagatgatgc ggaagtcggt tcactgattg ccgcgtacct ggcaaaacat
    42301 gatatgcagg ttaccgtaga gccgcgcggc gaccatgccg aagaaaccat tttgcgagaa
    42361 aatccggatt tggtgttact cgacatcatg ctaccaggca aggacggcat gaccatttgt
    42421 cgtgatttac gcgcaaagtg gtccggacca attgttcttc taacctctct cgatagcgat
    42481 atgaaccaca tcctggcact ggaaatggga gcctgcgact atattctcaa aacgacgccc
    42541 cctgctgttt tgttagcgcg tttacgtttg catttgcgtc agaatgagca agccacgctg
    42601 accaaaggtc ttcaggaaac gtcactgact ccctacaaag ccctgcattt cggcacgttg
    42661 accatcgatc ctatcaaccg cgtagtcacc ctggctaaca ctgaaatctc gctctcgaca
    42721 gctgatttcg aattattgtg ggaattagct acccatgccg ggcaaatcat ggaccgcgat
    42781 gcattgctga aaaatttacg cggcgtcagt tatgacggcc tggatcgtag cgtggacgtg
    42841 gctatttcgc ggttaagaaa aaaactgctc gataacgccg cagaacctta tcgcattaaa
    42901 actgtgcgta acaaaggcta tctttttgcg cctcatgcat gggaataagc gatgaaaaaa
    42961 ctgtttatcc agttttacct gttattgttt gtctgcttcc ttgtgatgtc tctgctggtt
    43021 gggctggtgt acaaatttac cgccgaacgc gcgggcaaac agtcgctgga tgatttgatg
    43081 aacagttcgc tgtatctgat gcgcagcgaa ttgcgtgaga tccccccaca cgattggggt
    43141 aagacgctga aggagatgga tttaaatctc tctttcgatc tgcgagtcga gccactgagt
    43201 aaataccatc ttgatgatat ttccatgcac cggctgcgtg gcggcgaaat tgtcgccctg
    43261 gacgatcagt acacgttttt gcaacgtatc ccgcgcagcc actacgtgct ggcagttggt
    43321 cctgttcctt atctttatta cctccatcag atgcgattgc tggatatcgc cctgatcgct
    43381 tttattgcca tttccctcgc ctttccggtg tttatctgga tgcgtccgca ctggcaggat
    43441 atgttaaaac tggaagcagc ggcgcaacga tttggcgatg ggcatctcaa tgaacgtatc
    43501 cactttgatg agggttcgag ctttgaacgc cttggcgtcg catttaacca gatggcggac
    43561 aatatcaacg ccttgatagc cagcaaaaaa cagcttattg acggagtcgc tcacgaactg
    43621 cgaacgccgt tagtgcgctt gcgttatcga ctggagatga gtgataacct gagcgccgcc
    43681 gaatcccagg cattgaatcg tgatatcagt caacttgaag ctttaattga agagttgcta
    43741 acttatgccc gactcgatcg cccacaaaat gagcttcatc ttagcgaacc agacctgccg
    43801 ttatggctgt caacgcatct ggcagatatt caggcagtaa cgcccgataa aatggtacgg
    43861 ataaaaacgc tcatgcaagg ccattatgcg gcgttggata tgcgcttaat ggagcgcgtg
    43921 ctggataatt tgctcaataa cgccctgcgc tactgtcact caacggttga aaccagcctg
    43981 ctactgtcgg ggaatagagc gacattaatt gttgaggatg atggcccagg gattgcccct
    44041 gaaaaccgcg aacatatctt tgaacctttt gttcgcctcg atcccagccg ggatcgctca
    44101 accggcggct gcgggctggg gttggcgatt gtccactcta tagcactagc aatgggcggt
    44161 acggttaatt gtgacaccag cgaactgggt ggtgcccgct tctcgtttag ttggccgtta
    44221 tggcataaca tcccgcaatt tacctctgcc tgacactatg cgcacggtgg tcaggctgtc
    44281 gatactgtgc tataaaataa agtatgttgt aactaaagtg gttaatatta tggcgcgtta
    44341 cgatctcgta gaccgactca acactacctt tcgccagatg gaacaagaac tggctgcatt
    44401 tgccgctgag ctagaacagc acaaattgct ggttgcccgc gtgttctctt tgccggaggt
    44461 aaaaaaagag gatgagcata atccgcttaa tcgtattgag gtaaagcaac atctcggcaa
    44521 cgacgcgcag tcgctggcgt tacgtcattt ccgccattta tttattcaac aacagtccga
    44581 aaatcgcagc agcaaggccg ctgttcgtct acctggcgta ttgtgttacc aggtcgataa
    44641 cctttcgcaa gcagcgttgg tcagtcatat tcagcgcatc aatcaactca agactacgtt
    44701 cgagcgtatc gtcacggttg agtcagaact ccccacagcg gcgcgttttg aatgggtgca
    44761 tcgtcatttg ccggggctga tcacccttaa tgcttaccga acgctcactg ttctgcacga
    44821 ccccgccact ttacgttttg gctgggccaa taaacatatc attaagaatt tgcatcgtga
    44881 tgaagtcctg gcacagctgg aaaaaagcct gaaatcaccg cgcagtgtcg caccgtggac
    44941 gcgcgaggag tggcaaagaa aactggagag agagtatcag gatattgcta ccctgccaca
    45001 ggacgcgaag ttaaaaatca aacgtccggt gaaggtgcag ccgattgccc gcgtctggta
    45061 caagggagat caaaaacaag tacaacacgc ttgccctaca ccgcttattg cgctgattaa
    45121 tcgggataat ggcgcgggcg tgccggacgt tggggagttg ttaaattacg atgccgacaa
    45181 tgtgcaacac cgttataaac cacaggcgca gccgcttcgt ttgatcattc cccggctgca
    45241 cctgtatgtt gcagattaac gccctgcttt catactgccg accatctgtt ctggccgtac
    45301 ccagctgtca aactcggttt cgctaagata ccccagcgca agggccgccg cttttaaggt
    45361 cagcccttct ttatgcgctt ttttggcgat ctcggcggct ttgtcataac caatgtgggt
    45421 gttgagcgca gtcaccagca tcagcgattc attgagtaat tgattgattc gctcacgatt
    45481 cggttcaata cccactgcgc agtgtttgtt aaaactttcc atgccatctg ccagcaagcg
    45541 caccgattgc aggaaattgt ggatcaccat cggacggaag acgttcagtt caaagttacc
    45601 ggaagcgccc cccatgttga ttgccacgtc gttccccatc acctgacagc agagcatggt
    45661 taatgcctca cactgggttg ggttcacttt ccccggcatg attgagctgc ccggctcatt
    45721 ttccgggatt gagatttcac caattccgca gcgcgggcca gaggccagcc agcggacatc
    45781 attggcaatt ttcatcagtg acgcagccaa ccctttcaac gcgccgtgcg cctgaaccag
    45841 ggcatcacag gtcgccagcg cttcaaattt gttcggcgcg gtaacaaacg gtgcgcaggt
    45901 aatgactgcc agttcatctg ccacgcgacg cgcatactcc ggatgggtat ttagtccagt
    45961 acccacagct gtaccgccca tagccagttc cgctacgtga ggcaggctgt attcaatatg
    46021 tttgagatta tgctcgagca tcgctaccca gccggaaatc tcctgcccca gtgttagcgg
    46081 cgtggcgtcc tgcaagtggg ttcgaccgat ttttacgata tcggcaaatg cacgggattt
    46141 ttcactcagc gtctgtgtca gggttttaag ctgcggaatg agttgcttgc gcagcgccag
    46201 cagcgccgca acgtgcatcg ccgttggaaa gacatcgtta gaactttggc ttttgttcac
    46261 gtcgtcgtta gggtgaactt tacgctccat cccacgcacg ccgccgagta attcactggc
    46321 ccggttagcc agcacttcgt tcatgttcat gttactttgc gtgccggagc cggtctgcca
    46381 gatagccaat gggaattcgt cgtcatgctg tcctgccagt acttcatccg ccgcctgccg
    46441 aatggcgctc gctttctctt cagacaacaa gcctaaatct tcattaactt ttgccgctgc
    46501 gcgcttggtt agcgccagcg catgaatcag tgaggtgggc attttctccg tcgaaatgcg
    46561 gaaatgctcc agcgagcgtt gagtttgtgc gccccacagc ttatctgccg ggacatcaat
    46621 cgcccccatc gaatcttttt cgctgcgtac tgtattcatg gcctgctcct cacctgatta
    46681 attttttctt tctgttttgc t
//
LOCUS       DACBVI010000020        44569 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.020, whole genome shotgun sequence.
ACCESSION   DACBVI010000020 DACBVI010000000
VERSION     DACBVI010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.020"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            213..2351
                     /locus_tag="IU325_05565"
     CDS             213..2351
                     /locus_tag="IU325_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP5808512.1"
                     /translation="MDIEIRHCNNIVRAHITLTADKLNIKFAPNGTGKSTLSRAISCA
                     ARDDIQGLQALMPFRLRGENPDSTGPIVIGADGIGDVMCFNEEYVSQFTFQPDELISD
                     SFNILIRNQAHAEREREIEEMTQKIRAVFTDHTELNSLIDHLQELSNAFKSTSSGISR
                     SSTGMRGLSGGNKIHHIPAGLENYQPYIRSERRVEWIDWQTKGLEFSPLSDGCCPFCT
                     GDITGKEAQIRQVREEYDKSTIKNLTAIIRLVENLGNYLTESARERLLAITMLQNGPE
                     AEHIEYLVALKRQTDTLTEKLTALRGLNVFSLQEQQNVREVLTARLIDLQFFPDLQSE
                     LMQGITDRLNAALMDLINLAGPLQGKINRHRDSMIRLIAQHKTNINNFLTYAGYKYRV
                     DIAGEGEQRKLRLRHIDFDGYVSGGSQHLSYGERNAFAIVLFMYECLSKNPGLIILDD
                     PISSFDKNKKFAILEMLFRRASGECLKNRTVLMLTHDVEPVIDTLKSVRRLFSNQVTA
                     SCLRLSAGVIEELPVNDGDIMTFMQICKSITASADCEEIIKLIYLRRYFEIVDERGDA
                     YQLLSNLFHRRVAPLDYREPAAAGSGYPKMAPEKIQQALRDIREYVDSFDYPRLQALV
                     SSPDEIKNLYRRCRNGYEKLQVFRLLELDQDHPVIRKFVNETYHIENEFICQLDPSRF
                     DLIPEYVIMECDKLIALPPAANQSSVARIA"
     gene            complement(2513..2929)
                     /locus_tag="IU325_05570"
     CDS             complement(2513..2929)
                     /locus_tag="IU325_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HAP5808513.1"
                     /translation="MNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRAAVDATTKIKVALRLAGTPIGPN
                     DTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK"
     gene            complement(2926..3156)
                     /locus_tag="IU325_05575"
     CDS             complement(2926..3156)
                     /locus_tag="IU325_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HAP5808514.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFLEYEKADPDFMAEREDVVSDEGRFNL"
     gene            3462..6581
                     /locus_tag="IU325_05580"
     CDS             3462..6581
                     /locus_tag="IU325_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808515.1"
                     /translation="MFGKLLKSVSWQVRAELRRSLKSNRDYKKLRWNPVERILVSCST
                     HYVRAMLVLWSAAFGAVGVVEYFRPVLLPFAVQHFKGITKLSDWMSNLLGSQLTIIGI
                     VFPLVVGLISVLFQKKSARIHIQSAYQLHSGYMFAGLSGLSLAAFVVLGGMTLSIGDG
                     YLNTSFAVTAFVWMLFNIILSIWFFVSSLNVLDESKRDRLMNKFFLSQIVDDYIQKAY
                     IQAWLRYPGVHVGQNYLGNIKILPYSISEKDDMLHVKSNISKGDVVTDIYIRPFLFLL
                     RRLEAVDGQDAEIIILPSFGVRSGELTLLSSRNVKPVSGLWRWLLRRCIVTGRPENKR
                     DLDDITFDFFGEAYDALNDKNISVFRTGIERLTDTYTSIKRSYNYEVDKNYLDEVKES
                     GFSHTFSDSFHYELRKFFRESVKSTEYSGEYFRESMLIPLRVYRKTQSTCFTDFRQFL
                     LSLFRVWHVLNEWKAGLGGPLSASQELTHQALIRGYIGLWEGWSMTTITGKPGSEDST
                     GRLMYHLHNTARLLIPSVVADNASSVRYAHDVLCLWFNQSRFTRYWEEEYRWHSFFLT
                     PDYLSLKETEPQWNMLLRGSKYKKDAALSIMFANALSDLRLLMAGYLIAHFESQKNID
                     LADLVNHLIMSELYEDRDTHDTLTPAFRRSVDIIDMILRIEHCNLHTNTSWYSGLSET
                     IEVMNSYNERPYIPGRMYTGEYEDLGSLYGSFALLAIKLARPAEQVTQRVNEALAGGV
                     FSYSSKDRIISILKRLKRDPSVPYEGYIISEADYATNVVFFNDVLDKYIDVFSRSKTA
                     DIVAAEVDQARLRNTDARLTNELPGALSEDVLLKYFTFTQNSECDRNWLAIYIPVGVS
                     KEYVARELNQTDYGDFPSVSEVNRNILRRLHYVLWQSQAKLTIEVNNLETLLMEVAQR
                     SADQNNYILVIYGSRFSEELRELVYQPERHDAFSIHVDVSARGSRSLPFRINNCLIYL
                     VLNSEQEFSLMVSAESFGELRLFRYPDGTLFNTFYRSSDDPLEGVMKTLWEIEMEITD
                     TPVARFEHR"
     gene            6844..7977
                     /locus_tag="IU325_05585"
     CDS             6844..7977
                     /locus_tag="IU325_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HAP5808516.1"
                     /translation="MHFYVDETGQTGRNLFDKTQPILPYGVLSSEADLDKVAEADLAA
                     LRKTLGVERLHAAELGVHRLSEVVDTLLVLQKNHRIRFDIWQVVKRDHAIISFFDQVF
                     DQGLNPVVPWSAYWTPLRYPLLLNLSNLFDDELAEKAWRARLEAHDERSCSLFSEVCG
                     VLLQRVHSLGDARSVELITDALSWAMVNFDELGYNCKTNKEKLQIMPNMIGFQSVLHG
                     ICSRLGAPNRKADIIVDQQSQFNTTQRELNEFYYQIREQPWALGPGLPVMDMKNMPAK
                     PLVFQSGTMSAGLELVDIYLWIFKRYMERKELTKPLSRLVYTNLKTARTDSVSLQSVA
                     KRFKEFFEKLSEPTAEMIEKANELRAVEETRRLAHRVQSVSQS"
     gene            7997..8281
                     /locus_tag="IU325_05590"
     CDS             7997..8281
                     /locus_tag="IU325_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="korC"
                     /protein_id="HAP5808517.1"
                     /translation="MKAENARQVQGLIELEKFNPETLCSGESWMAPSASEVSVVRALI
                     PLTDIQLANRLDVDERTIRKWKSGETRMVFTTWCCLCWLAGLGMLLEEPA"
     gene            complement(8278..8475)
                     /locus_tag="IU325_05595"
     CDS             complement(8278..8475)
                     /locus_tag="IU325_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000215657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808518.1"
                     /translation="MTVFLMAVFYGLLKTGSLIALLVDAYKGWTLFWKNYDRLPHIYR
                     SQWRYQRRFILKRIWKRIRTG"
     gene            8785..9276
                     /locus_tag="IU325_05600"
     CDS             8785..9276
                     /locus_tag="IU325_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000528930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808519.1"
                     /translation="MGNYSDFETDFIERTLALIDQYNNMIEGKPFPEQYNYTLTLNCL
                     LGLIVMPRERAVSYLPSDRLTPELKAEIGLNESQLPGEEMNLRELIHKMRNSVAHFCV
                     QVESISDARLVDQIIFKETHGAGRAYAIFSAPELLPFLKYYAALLIANMRRHRGVPTT
                     DVV"
     gene            9288..10046
                     /locus_tag="IU325_05605"
     CDS             9288..10046
                     /locus_tag="IU325_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808520.1"
                     /translation="MFTLDCSTRSQALLSLSSGFGCSVMELKKVLLSLDLEQIYETDH
                     SIMIDSRQYLREYVCRELGIPGEFTTAYWFHGTRTSADNIFENGLLALNQTESLVMDM
                     LVNLAPDAEVKEKLQAWNFHAGVPDHLFRTRTRDKMHWGPYGHLVREVHLHARKLWQH
                     DYVRLPELVEDVCNAYKKKYGQDLTGHYLEVLKPCIVCFRADIDYEKGALEAALSYAY
                     TSVRELPPDSGAVFGIDRHGKSVSVDEIVNVEFI"
     gene            10268..11860
                     /locus_tag="IU325_05610"
     CDS             10268..11860
                     /locus_tag="IU325_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808521.1"
                     /translation="MTSIIVTSLLKTGPCLSSVLVEEMLKTSGVNRDTARKQISRAAS
                     AGQIHCVDKLFPKRERFIYLKQEYGTGRFWSSLNAALLDTGSAYGLALSCLRARGGIL
                     PVRNFSAACGSPVAMKNRLSWKSVLDGLLQYKMVRVVTLPGLGECVALTEKNDNGYLR
                     ALHPLKARLLTESVLMKSLSQWVRNNGIISYDTLRTREELNSDQTPCVANFDFDVTAA
                     SYLNPLLQFSRSGEIRPGFFVCDMLLGCKLSLVHLQPFITKCRSINSLRNSPRCLFMF
                     IADEYSEEAFLEMKRAGIIPATPENLFGKDFADALFQLRDLVGSITLSLKDNIAAIDD
                     IMSKLANIAGATNQLQGDLFEYIVAETVRIDSKDVEVGKICKSLKGETAECDVLSLNG
                     HAKITFIECKGYKPYSTVRHEDVKKWIGKQVPVFFSYAKREYPNAEINVQLWTTGKLC
                     DDSRESLRKFQENNLTNQRYNITVMEPHEVCARIKATRNDALIRVFDKHFLSYPEKIV
                     RRKHVPDPVRLAGHDEAIEFDF"
     gene            11895..13019
                     /locus_tag="IU325_05615"
     CDS             11895..13019
                     /locus_tag="IU325_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HAP5808522.1"
                     /translation="MDYTKNQHVLSQWVLRNFRSDDTALYAKEKQRVWCHTVYMTPEK
                     ENVLHTQPLPISSVAVSKNCFRLIDAETGQPFDIEDELGVYERLLAGIVNDIIHEHNF
                     SRLRHTHPDDFPVEKLTSFAVMQLMLNLHNPQNKNPYKQEMLEQFHADIQDHLSEYIH
                     SINQLPHSNPELMDDHFYRKILRVANSASSDENKCRALFVLFGLLSTLNKPTLYDKIN
                     KLRNNIFTGIHTIEVIHTGHDFDSTEPRPAFAIAPNIFCIYKDENRIYLPLSHNITLC
                     FITGTALHFRPRIDVFSPRPERLKCCHDRSLNFYNVSFDYIDNVMTTIDMYNVGTSST
                     FYSAYELSKLNEYLDKQQQDHDYYYTPENPVKWQPAQTVT"
     gene            13019..13309
                     /locus_tag="IU325_05620"
     CDS             13019..13309
                     /locus_tag="IU325_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="korC"
                     /protein_id="HAP5808523.1"
                     /translation="MKAVNENDFRELIEAKQFNGYTLVSGEDWQIPTAHEILLVRGLI
                     PLTDIQLANRLDVDERTIRKWKTGQTRMVFTTWCCLCWLAGLGMPLDNHLSG"
     gene            13882..14883
                     /locus_tag="IU325_05625"
     CDS             13882..14883
                     /locus_tag="IU325_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HAP5808524.1"
                     /translation="MADSVNLQTDVVRQHTVPRFLLKHFSTPGKGKRQRLYAFDKAAG
                     RAYATTPDDATVRNTFYNLENHPDRLSLEPLLGIYEHHAAPVIAALLAHRDIRRLTDD
                     ERYRLAVFVAVQRARTFGELERISGMISVLTDKMEAIGSTKEQALETLGLSSGGDTKD
                     IFLRQLVQQMSHIDHLLKKDWYLLETRPESPFYVSDNPVVLKNSNDFGPYGNLGLAVR
                     GIQIYLPLSSTLMLAMYCPSIREQMIRQKQHLHNLLARAPHLIPRHIRPFERLEHIRR
                     YTDYLFMPLTPDHVTHYNALQVEFAEQYVFCGEKDFSLVERMLADSERYRTGPRFTF"
     gene            complement(15057..15401)
                     /locus_tag="IU325_05630"
     CDS             complement(15057..15401)
                     /locus_tag="IU325_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP5808525.1"
                     /translation="MKNLFDTEGTVLFPFQTEIHFIWTIYTTAKTLYVSACGHIGQKQ
                     YWSACLCILLLMAYVGLCAAVVWAVVPWLKAFIVSKFAVSALWVTPVKSLLLRGIGKM
                     VGAICRKFTLLK"
     gene            complement(15899..16153)
                     /locus_tag="IU325_05635"
     CDS             complement(15899..16153)
                     /locus_tag="IU325_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808526.1"
                     /translation="MKGRQSRYVTGGESFAEIARRPAGTVVILSLNTDLEDALREVSK
                     SLKSAFCRCGRKCQLSAGTSEGPFSGRRQGVATHLFVSVL"
     gene            complement(16203..17129)
                     /locus_tag="IU325_05640"
     CDS             complement(16203..17129)
                     /locus_tag="IU325_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808527.1"
                     /translation="MSEKEHDMTNDGGESVTYTLRNIPADIDRVITDLATHARKPKAT
                     FLREFLEDSFRDVIDGFALKNPLIASLDDELASYLGAEVMEKRYQSHYITRWNQEYQK
                     LLGISTEEELRRVVLTNTPFLHARADQVLKGWKKIPRGISLTFSLFAEIAGRDRETID
                     SAWNRIFYSQLREKKHRFYRDIDVIRALKKQHAVCGYSWTRDDITVRIYRPDDYGYGA
                     WRVLLVLDESVITQTWNIPFPELDGRRFTTDPGYDALISTAPDSWDKAFRFVDGICEL
                     HLYSNGVEEDQNPTPLPAVAEALIEAVVHDLL"
     gene            17662..17976
                     /locus_tag="IU325_05645"
     CDS             17662..17976
                     /locus_tag="IU325_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808528.1"
                     /translation="MGKPTFRSFYDVVRELEDVYGHKELWLYSGAAYATPTEMINARH
                     NWKSPKILKRNGRMVAERMDNSDSWQLVGDYKKPLFQHCAPPWQSCQIDDYFKGYYII
                     AP"
     gene            18029..18808
                     /locus_tag="IU325_05650"
     CDS             18029..18808
                     /locus_tag="IU325_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808529.1"
                     /translation="MGSMKELLFEMQEERRDEWIAENYPDAEEGTPEWDAAAQEYSWF
                     QDWMEEAAEQQYFEASLASIPDRLQDAKAELDELESLMQFNQPRIVERMAYVHCVSVL
                     DSFLMYSARALLSHPPHLQKFLHEADSLVPNKEDRRKLLASKWVEQEPDKDTPEKVYT
                     WRAQSLVAKKTFQSHKVIGWYFSRMLTTPHEWPLEEIKGVIKIRNALVHRNGVTESLE
                     PVYISSGSVQNAICTVRAFITVAAETLLQEDALYRTDDGIF"
     gene            18859..19638
                     /locus_tag="IU325_05655"
     CDS             18859..19638
                     /locus_tag="IU325_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP5808530.1"
                     /translation="MTSLPANCIPVSNTSLPVAIDYPAALALRQMALVQDELPKYLLA
                     PEVSALLHYVPDLHRKMLLATLWNTGARINEALALTRGDFSLAPPYPFVQLATLKQRT
                     EKAARTAGRMPAGQQTHRLVPLSDAWFVSQLQTMVATLKIPMERRNKRTGRTEKARIW
                     EVTDRTVRTWIGQAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMGH
                     KSISSTEVYTKVFALDVAARHRVQFSMPESDAVTMLKNRHA"
     gene            complement(19776..20051)
                     /locus_tag="IU325_05660"
     CDS             complement(19776..20051)
                     /locus_tag="IU325_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HAP5808531.1"
                     /translation="MVKKPSQQALNRAAVTVEQAEALAQRLADKPYGAPEKPEPEKQC
                     RTTISLGESMLVTIEDLALRNKRNGKDPKNVSAIVRVALEQYLKTLT"
     gene            complement(20045..20689)
                     /locus_tag="IU325_05665"
     CDS             complement(20045..20689)
                     /locus_tag="IU325_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP5808532.1"
                     /translation="MITVVGGNKGGSGKTTIASNLAIALANKGREVCLLNGDLQRTAA
                     KHHAEREAAGLLPAITLVEKFDNLTQTLQALNEKFDDVIVDVAGRNSKEFITSGVVAH
                     QIIAPLQCSQPDLDTLTELEQQIDAMRNLNPELKVYCLQSMATTNPVLRGNERKEFLE
                     YLEEFPTIQVLDSVICFRKVYRDCMSNGTGVVETNNTAARAEIEHLMNEVFGPW"
     gene            20918..21889
                     /locus_tag="IU325_05670"
     CDS             20918..21889
                     /locus_tag="IU325_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein parM"
                     /protein_id="HAP5808533.1"
                     /translation="MNVYCDDGSTTIKLAWNDNGKICKSLSQNSFRHGWKVDGLGIRQ
                     TFNYELDGKKYTYDEVSNQSILTTHIEYQYTDVNLLAVHHALLNSGLAPQPVSLTVTL
                     PISEFYTKECQKNELNIQRKIENLMRPIRLNKGDVFTIEHVDVMPESLPAVFSRLVMD
                     KVGQFEKSLVVDIGGTTLDVGVIVGQFDSVSAIHGNSGIGVSSVTKAAMSALRMASSD
                     TSFLVADELIKRRNDPDFVRQVINDETKTDLVLNTIEGAIASLGEQVVNELGDFHHVN
                     RVYVVGGGAPLIYDSIKTAWHHLGQKVVMMESPQTALVEAIAAFKEE"
     gene            21894..22286
                     /locus_tag="IU325_05675"
     CDS             21894..22286
                     /locus_tag="IU325_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stability protein"
                     /protein_id="HAP5808534.1"
                     /translation="MDDERKRKKFTLYLHPEKAADFQTLEAIESVPRSERGELFRNAF
                     ISGMALHQLDPRLPVLLTAILSEEFSADQVVTLLSQTTGWKPSQADIRAVLTELGALQ
                     SAEKMPPSATDSVQEAMNDVRLKMQKLF"
     gene            complement(22291..23562)
                     /gene="umuC"
                     /locus_tag="IU325_05680"
     CDS             complement(22291..23562)
                     /gene="umuC"
                     /locus_tag="IU325_05680"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
                     /protein_id="HAP5808535.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSPSYTTQWTEIPMASF"
     gene            complement(23562..23999)
                     /gene="umuD"
                     /locus_tag="IU325_05685"
     CDS             complement(23562..23999)
                     /gene="umuD"
                     /locus_tag="IU325_05685"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAP5808536.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(23996..24244)
                     /locus_tag="IU325_05690"
     CDS             complement(23996..24244)
                     /locus_tag="IU325_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ImpC"
                     /protein_id="HAP5808537.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            24593..25564
                     /locus_tag="IU325_05695"
     CDS             24593..25564
                     /locus_tag="IU325_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HAP5808538.1"
                     /translation="MRHGLMEAACERRIPMPNWCSNRMYFSGEPAQIAEIKRLASGAV
                     TPFYRRATNEGIQLFLAGSAGLLQITENIRSEQCPGVTAAGRGAVSPENIAFTCWLTH
                     LQNGVLLDEQNCLMLHELWLQSGTGRRRWEGLPDDVRETITVHFTAKRGDWCDIWGNE
                     DVSVWWNRLCDNVLPEKTMPFDLLTVLPTRLDIEVNGFNGGVLNGVPSAYHWYTERYG
                     VKWPCGYDLNISSQGENFIQVDFDTPWCQPESDVVAELSRRFSCTLEHWYAEQGCDFC
                     GWQLYERGELVDVLWGELEWSSPTDDDELPEVTGPAWIVDNVAHYGG"
     gene            25568..25872
                     /locus_tag="IU325_05700"
                     /pseudo
     CDS             25568..25872
                     /locus_tag="IU325_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25949..26632
                     /locus_tag="IU325_05705"
     CDS             25949..26632
                     /locus_tag="IU325_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HAP5808539.1"
                     /translation="MSRFVLGNCIDVMTRIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            26633..26854
                     /locus_tag="IU325_05710"
     CDS             26633..26854
                     /locus_tag="IU325_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808540.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDACFKD"
     gene            26868..27302
                     /locus_tag="IU325_05715"
     CDS             26868..27302
                     /locus_tag="IU325_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HAP5808541.1"
                     /translation="MYGTCETLCRELAAKYPGDTPLMLVIWSPEEIQALADGMEISLT
                     DHEIRTVLARMEDIPEDQRTESGISSAAVMEIISNVSENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDNGLVVPECVTRRQAVVNQARALLKNNTREND"
     gene            27302..28111
                     /locus_tag="IU325_05720"
                     /pseudo
     CDS             27302..28111
                     /locus_tag="IU325_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001476318.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27996..28568
                     /locus_tag="IU325_05725"
     CDS             27996..28568
                     /locus_tag="IU325_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1472 domain-containing protein"
                     /protein_id="HAP5808542.1"
                     /translation="MCCVYPMNRPAAGLTVVFCGRWSGKPGPKSAAWRMPWQKSGNDD
                     TGGVNPRLFPAGPRTEHKGSRRRLRFTRPCGAWPCGVSVMWPQRRVRAVPCLHLSRAG
                     VDARVRFAAAVTRSLLPVCRDFPVVHPLRFRGLTLQLPPAVCVRLRLPLRPVHPRLIA
                     RLLWRHGTARCRGICEQRRKTSCNMRNLSL"
     gene            28664..28966
                     /locus_tag="IU325_05730"
     CDS             28664..28966
                     /locus_tag="IU325_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAP5808543.1"
                     /translation="MMPEYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSA
                     DAAGIILTSLVINRQLWLYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            29013..29435
                     /locus_tag="IU325_05735"
     CDS             29013..29435
                     /locus_tag="IU325_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HAP5808544.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEYGADVSIVHDVVRELMEEKRASRHVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGETMS"
     gene            29432..29623
                     /locus_tag="IU325_05740"
     CDS             29432..29623
                     /locus_tag="IU325_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808545.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            30394..30921
                     /gene="ssb"
                     /locus_tag="IU325_05745"
     CDS             30394..30921
                     /gene="ssb"
                     /locus_tag="IU325_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP5808546.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSETWRDK
                     QTGEMKEQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTVQMLGRAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     QQPEPPYGFDDDAPF"
     gene            30978..31211
                     /locus_tag="IU325_05750"
     CDS             30978..31211
                     /locus_tag="IU325_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HAP5808547.1"
                     /translation="MNKSDEMIQELPDGAFTRECAETVVTQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDCAGYWMNRYIRDFGIRK"
     gene            31273..33231
                     /locus_tag="IU325_05755"
     CDS             31273..33231
                     /locus_tag="IU325_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP5808548.1"
                     /translation="MSVVKSEPDTTRKASRKSAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERNILTADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEYLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEIRAGSGVVVSWCYGNV
                     CVQRGVQLRSEDDVADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSSPARIYLECGHYSLTSAAPSGKEGTAFMALMAEHFRL
                     AALLPDEWERDMTTFLSLSQEVLLSLLSFCTACSIHGVQTRECGHTSRSPLDSLETAI
                     GFHMRDWWQPTKANFFGHLKQPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCAPRPQTDATEHTANLADAA"
     gene            33286..33720
                     /gene="psiB"
                     /locus_tag="IU325_05760"
     CDS             33286..33720
                     /gene="psiB"
                     /locus_tag="IU325_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HAP5808549.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNCGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            33717..34436
                     /locus_tag="IU325_05765"
     CDS             33717..34436
                     /locus_tag="IU325_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HAP5808550.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENAI"
     gene            34433..35029
                     /locus_tag="IU325_05770"
     CDS             34433..35029
                     /locus_tag="IU325_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808551.1"
                     /translation="MMKSDEQYQVPVWMRPLLPLLCNTGGNDPEELLNDTETTASANI
                     VRYVLIVAVRSQIDLLQLLYRKGLLRTEIPGGFSPEEAQELLDNLVRSHISKALSGER
                     MAARDRNADLTWIRQQLVDAAWFVRATLEAHGMGVGNESPSAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSSVVREAQDWLCDRKLVDITDILR"
     gene            35133..35571
                     /locus_tag="IU325_05775"
                     /pseudo
     CDS             35133..35571
                     /locus_tag="IU325_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003914613.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1472 domain-containing protein"
     gene            35492..35992
                     /locus_tag="IU325_05780"
     CDS             35492..35992
                     /locus_tag="IU325_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP5808552.1"
                     /translation="MSVVAPALYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRQARDEGCEEAYRLWVEDTGETD
                     FDTFRDAWWGEADSEEAFAVEFACDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            36721..37155
                     /locus_tag="IU325_05785"
     CDS             36721..37155
                     /locus_tag="IU325_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HAP5808553.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            37248..37514
                     /locus_tag="IU325_05790"
     CDS             37248..37514
                     /locus_tag="IU325_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808554.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            37579..38481
                     /locus_tag="IU325_05795"
     CDS             37579..38481
                     /locus_tag="IU325_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAP5808555.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGALEKALAIACQLQK
                     MGMTPEQIRQATGLSDDELKKITH"
     gene            38505..38750
                     /locus_tag="IU325_05800"
     CDS             38505..38750
                     /locus_tag="IU325_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808556.1"
                     /translation="MPGFLSSRSRANSMTLAERLIQKGFDEGFKEGFKQGFKQGALEV
                     AREIACRLRDIGWPPERIQEVTGLSGEELKKLFPDEQ"
     gene            complement(38781..39032)
                     /locus_tag="IU325_05805"
     CDS             complement(38781..39032)
                     /locus_tag="IU325_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP5808557.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMHKADIIRRLENELGETAEDV"
     gene            39612..40460
                     /locus_tag="IU325_05810"
     CDS             39612..40460
                     /locus_tag="IU325_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HAP5808558.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRRMDKPEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRSDLVEKLNDIIARHYPG
                     VLPSRV"
     gene            complement(40546..40881)
                     /locus_tag="IU325_05815"
     CDS             complement(40546..40881)
                     /locus_tag="IU325_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HAP5808559.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            41115..41447
                     /gene="nikA"
                     /locus_tag="IU325_05820"
     CDS             41115..41447
                     /gene="nikA"
                     /locus_tag="IU325_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxosome accessory protein NikA"
                     /protein_id="HAP5808560.1"
                     /translation="MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSA
                     YIRNAALNKRINSRTDDAFLKELMRLGRMQKHLFVQGKRTGDKEYAEVLVAITELTNT
                     LRKQLMEG"
     gene            41458..44160
                     /gene="nikB"
                     /locus_tag="IU325_05825"
     CDS             41458..44160
                     /gene="nikB"
                     /locus_tag="IU325_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxase NikB"
                     /protein_id="HAP5808561.1"
                     /translation="MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQ
                     PHRSRFSRLVDYATRLRNESFVALVDVMKDGCEWVDFYGVTCFHNCTSLETAAADMEY
                     IAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVRHTLKSLGLGEHQYVSAVHTD
                     TDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRI
                     VRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQ
                     MDGKFLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVDAPGRYNP
                     DFIAADVRPEKIAETESLQQYACRHLGERLPEMAREGRLENCQAIHCTLAEAGLWMRV
                     QHGHLVICDGYDHNQTPVRADSVWSLLTLDNVNQLNGGWQPVPTDIFRQVTPTERFRG
                     RRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGYSISHCSPQIEEMITQGEF
                     TWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEPQAGPFV
                     SAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAW
                     REQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLK
                     EDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTT
                     DRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGEFVCTDYGDRIVFRNHHDR
                     NALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAWYNVTGRTGHEDYRITR
                     PDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKLWEPPSPMR"
     gene            44283..>44569
                     /locus_tag="IU325_05830"
     CDS             44283..>44569
                     /locus_tag="IU325_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001760750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HAP5808562.1"
                     /translation="MSTNLAFRYASVETFFKMIENQELWFTDLRNMNDPEEYSAGYEI
                     VKRIINEEFPDCIRLLEEVSPNKIDENFKILICCFCSDGDRSNMWSEYGDNG"
BASE COUNT        10694 a        10982 c        11878 g        11015 t
ORIGIN      
        1 cttaatcatc atatttcctg cctgttcccc gccgcgcaga tccggtttcc ggcaaagtcg
       61 gatcgaccta atctttgttt ctgatatgaa catctcggag aggtgtaagc aatggtataa
      121 acgaaatgta ttatgtatct gcatcacagc atgcgggcac attttgcggc aggatatgaa
      181 tccccccgga ataactggca aaggacagat aaatggacat tgaaatacgc cactgcaata
      241 acatcgtacg agcacatatc accctcactg ccgataaact gaacattaag tttgcgccaa
      301 atggtactgg aaaaagtacg ctgtcacggg ccataagctg tgcggcgcgg gacgacattc
      361 agggactaca ggcactgatg cccttccggc tgcgcggaga aaaccccgat agcaccgggc
      421 ccattgtcat cggtgctgac gggattgggg acgtgatgtg cttcaacgag gagtacgtca
      481 gtcagtttac gttccaaccg gacgagctca tcagtgacag ctttaatatt cttatccgca
      541 atcaagccca tgcggaaaga gagcgtgaaa tagaagaaat gacgcaaaag atccgggctg
      601 ttttcacgga tcacactgag ctgaactctc tgatagacca tctacaggaa ctcagtaatg
      661 ctttcaaatc aaccagttcc gggatttcac gttcttcaac cggcatgaga ggtctgtcag
      721 gcggaaataa aatacaccac attccggctg gtcttgagaa ctatcaaccc tacatccgca
      781 gtgaacgccg cgtggaatgg attgactggc agacaaaggg actcgagttt tcgccccttt
      841 cggatggctg ctgtccgttc tgtaccggtg atatcacggg aaaggaagca caaattcgcc
      901 aagtcagaga ggaatacgat aaatccacca ttaaaaacct gaccgctatc atcagactgg
      961 tggaaaacct cggtaattac ctgacggaga gcgccagaga acgccttctg gccattacta
     1021 tgcttcagaa cggtccggaa gccgaacata tcgagtacct agtggcgttg aaacgccaga
     1081 ccgatacgct gacagagaaa ctcactgcgc tgagaggcct gaatgttttc agcctgcagg
     1141 aacagcagaa cgttcgggag gtgctcactg ccaggttgat tgacctgcag ttcttccctg
     1201 atctgcaatc cgaactcatg caggggatca ccgacagact gaacgcggcc cttatggacc
     1261 tgataaacct tgccggaccg ctgcagggaa aaattaaccg gcaccgggac agcatgatcc
     1321 ggctgatcgc acagcacaaa acaaacatca ataatttcct cacttatgca ggctataaat
     1381 acagggtgga tatagccggc gagggagagc agagaaaact caggctacga cacatagatt
     1441 ttgacgggta cgtcagcggc ggtagccagc atctcagcta tggagaacgg aacgcctttg
     1501 cgattgtgtt attcatgtat gaatgcctgt caaaaaaccc gggactgatt atccttgatg
     1561 atcccatatc ttcttttgat aaaaacaaga agtttgccat tcttgaaatg ctgttcagac
     1621 gtgcaagtgg tgaatgcctg aagaaccgga ctgtacttat gctgacgcat gacgtggagc
     1681 ctgtgattga cacgctgaag tcagtcagaa ggttgttcag taatcaggtg accgcctcct
     1741 gcctgcgcct gtctgctgga gtcatagaag aattacctgt taacgacggc gatatcatga
     1801 cattcatgca gatctgcaaa tccatcaccg cgtcagcaga ctgtgaggag atcattaagc
     1861 tcatctatct tcgtcgctac tttgaaatcg ttgacgaacg tggtgatgct tatcagcttc
     1921 tatccaatct attccatcga cgcgtcgccc cgctggatta tcgcgagcct gccgctgcgg
     1981 gctcgggcta tcccaaaatg gctcctgaga aaattcagca ggccttgcgg gatatccggg
     2041 agtatgtgga cagttttgat tacccgcgac ttcaggctct cgtcagcagc ccagatgaaa
     2101 taaaaaacct gtaccgtcgc tgtcgtaacg gctatgaaaa actgcaggta ttccgtttac
     2161 tggaactgga tcaggaccat ccggtaatac gaaaattcgt taacgaaacg taccacattg
     2221 aaaatgagtt catctgtcaa ctagatccgt ccagattcga tctcatcccg gagtacgtga
     2281 ttatggaatg tgacaagctc atcgccctgc caccggcagc aaaccagagc tcggttgccc
     2341 gtatcgcttg agccactgtt tttgccgccg gaagataggc tttagctccc tgatagtctg
     2401 atcttcgaag ataccctaca gtttaggacc gcttactgta gatatctgtt aaaacagccc
     2461 gtatacgggc tgtttcttgg tgcccttatc tcctgacttt gaaaagagtg tcttacttca
     2521 cccagtcttc cagcaccagg tcaggcactc gctcaaattc tcttgtatta ttcgttacca
     2581 gtatcgcccc ggcggcgatg gcgtgcccgg caatcgccgt gtcgttcggg ccgatcggcg
     2641 tcccggccag ccgcagtgcc actttaatct tcgtggtggc gtctactgcg gcgcgatccc
     2701 agggcaggat ggcatcgagg cgggcgcaga attcatcgac cagttggacg tgacgtggtg
     2761 aggcctttgg gccggtagcc ccgaagcgca tttcagagta ggtgatggcc gagaccacta
     2821 tacggtggcc gcgtagcacc gcctgctcca gacgcttcag gacggcttct ggctgttcac
     2881 gcatgatgaa cgagcagata caggtatcaa gcatataagt cttgttcaca ggttaaatcg
     2941 cccttcgtcg ctgacaacat cctcgcgctc cgccataaaa tctggatcgg ctttttcgta
     3001 ctctaggaat gatccccagg ttggccggac ggggcgcagg atgatgctgt ccccttcccg
     3061 gacgatctcg agctcgctca ccccctcaaa atccagatca cgaggcagac ggatggcgcg
     3121 gttgttgcca tttttaaata tcgatacggt tctcatgttc tttctctccc gagcatatgg
     3181 atatgtgaaa catatccgga atattcaaag caccgggtgt gtgcatatgt ttagtatagg
     3241 cagatgtgtg gcatatgtat agcggtagca tatgtagatg ccaggcatat gtttagcata
     3301 tctctatttc tttctttttt atcggagatc cgaaaaaagg ttaaaccgga ttttaggatg
     3361 acaaagcctg tatgaagggt atcgggtgac attttccaga gaaaaaaagg ttcctaccgc
     3421 ttattattca taaaatttct tatgtaatac gcaggatatc tatgtttgga aaacttctga
     3481 aaagtgtctc atggcaggtg agagctgaac tccgacggag cctgaagtcg aaccgggatt
     3541 acaaaaaact tcgctggaac ccggtggaga gaatacttgt ttcctgctcc acccattacg
     3601 tccgggctat gctggttcta tggtccgcag ccttcggggc tgttggcgtg gtcgaatatt
     3661 ttcgccctgt attgctacct ttcgctgtgc agcattttaa gggcattacc aagctttcag
     3721 attggatgtc taatttactg ggtagccagt tgactataat cggtattgta tttccgctgg
     3781 ttgtggggct tataagtgtt ttatttcaaa agaaatcagc aaggatccat attcagtccg
     3841 cgtatcaact tcattccgga tatatgtttg cgggcctgag tggtctttca ctggctgcat
     3901 ttgtcgtgct gggcggtatg acgttatcta taggtgacgg atatcttaat acttcttttg
     3961 ccgtaacagc ttttgtgtgg atgcttttca atattattct gtctatctgg ttcttcgtct
     4021 ccagtctgaa tgtcctggat gagagcaaac gcgatcgact aatgaataag ttttttttat
     4081 ctcagatagt ggatgactac attcaaaaag catatattca ggcgtggctg cgttacccgg
     4141 gggttcatgt aggtcaaaat tacctcggta atattaaaat actgccttac tcaatttctg
     4201 agaaagacga tatgcttcat gttaaatcta atatcagtaa aggtgatgtt gttactgata
     4261 tttatataag gcctttttta ttcctactga gaaggctaga ggcggtagat gggcaggatg
     4321 cggaaattat cattctgccc tcctttggtg ttcgttctgg tgaactgact ttattgtcat
     4381 cgagaaatgt caagcctgtt tcaggattgt ggcggtggtt attgcgtcgt tgtattgtaa
     4441 cagggcgccc tgagaacaaa agggatctgg atgatattac ttttgatttc tttggtgagg
     4501 cttacgatgc gcttaacgat aaaaatatta gcgtcttcag aacagggatt gaacgattaa
     4561 cagataccta tacctctatc aaaagaagtt acaattacga ggtcgataaa aattatctgg
     4621 atgaagtaaa agaatcaggt tttagccata ctttcagcga ttcattccat tatgaattga
     4681 gaaagttttt tcgtgaatcc gtaaaatcaa cggaatattc aggcgaatat ttccgtgaat
     4741 cgatgctcat tccattgcgt gtgtacagaa aaacacagag cacgtgtttt accgatttca
     4801 gacagttttt actctcgctt ttcagggtgt ggcatgtgtt aaacgagtgg aaagcgggac
     4861 tggggggccc cctctcggcc agccaggagc tgactcacca ggcccttatc cgcgggtata
     4921 tcggactgtg ggaaggctgg agtatgacca cgatcactgg caaacccggg agtgaagatt
     4981 ctactggccg cctgatgtat catctgcata ataccgccag gcttcttatt ccgtcagtgg
     5041 ttgccgataa tgcctcttcc gtgcgatatg ctcacgacgt tctctgcctc tggtttaatc
     5101 agagccggtt tacacgttac tgggaggaag agtaccgctg gcacagtttc ttcctgactc
     5161 cggattatct gtccctgaag gagacagaac cgcagtggaa tatgttactc aggggcagca
     5221 agtataaaaa agatgccgct ttgtccatta tgtttgcaaa tgccttatcc gatttgcggt
     5281 tgttaatggc agggtatctt attgctcatt ttgaatcgca aaaaaatata gacttagccg
     5341 atcttgtgaa tcatctcatt atgtctgaat tatatgaaga tagagataca catgacacgc
     5401 tgacgcctgc attcagacgt tctgtcgata tcattgacat gatactgaga attgagcact
     5461 gcaatctgca tacgaataca agctggtaca gcgggttatc agaaaccatt gaggttatga
     5521 actcatataa tgaaaggcct tacattcccg ggcgtatgta tacaggggaa tatgaagatt
     5581 tagggagtct ctacgggtcg tttgccttgc tggctataaa gctcgcaagg cctgcggagc
     5641 aggttacaca gcgtgttaat gaagccctgg ctggaggggt gttttcctat tccagtaaag
     5701 acagaataat ttctattctt aaacgactga aacgagaccc ttcagttccc tatgaaggtt
     5761 atatcatttc agaagcggat tatgcaacga atgtggtgtt ctttaatgat gttctggata
     5821 aatacataga tgtattcagt cgtagtaaaa cggcagatat cgttgctgct gaagtagacc
     5881 aggctcgact gagaaacact gatgctcgtc ttacaaatga acttcctggg gcattgtctg
     5941 aagatgttct gcttaaatac ttcacgttta ctcaaaattc agaatgtgat cgcaactggc
     6001 tggcgatata tattcctgtt ggtgtttcca aagagtatgt tgccagagag cttaatcaga
     6061 ctgactacgg agatttccct tcggtatcag aagtgaacag aaatattctc cgcagactgc
     6121 attatgtact gtggcagtca caggctaaat taacgattga ggtcaataac ctcgaaacgc
     6181 tgttaatgga agtcgcgcaa cggtccgctg atcagaataa ctacattctg gtgatctatg
     6241 gttcccgctt cagcgaggaa ctgagggaac tggtctatca gccggaaagg catgacgcgt
     6301 ttagcataca tgttgatgta tcggccaggg gtagccgctc tctgcccttc aggatcaata
     6361 actgtcttat ttaccttgtc ctcaattccg aacaggagtt ttcgcttatg gtcagtgctg
     6421 aatcctttgg tgaactcagg ctgttcaggt atccggatgg aacccttttc aataccttct
     6481 accgcagcag cgacgatcca cttgaaggtg tgatgaaaac gctctgggaa atagagatgg
     6541 aaatcactga tactccagtg gcgcggtttg aacatcgctg atcaaaatta cggtgacccc
     6601 tgatggggtc gctgtttaat tgcattagag ttatcaatta ctataaattt tgcttcctaa
     6661 gcaataatct attcaccgca acaatccgtc gctttttgtg ccattcatac aataatccct
     6721 cttctctctt ccttagatcc gaaaactcat ttaagaggat cacttgcagc cggcaacagg
     6781 aaggttttac attgatttga attatgccgt ttcttttcct cctgccttga aaaggatatc
     6841 tgaatgcact tttatgtgga tgaaacaggc cagactggcc gaaacctttt tgacaaaacc
     6901 cagccaatcc tcccctacgg agtactgtcc tcagaggctg acctagataa ggttgctgaa
     6961 gcggatcttg ccgcattacg caaaactctg ggcgtggagc gcctgcacgc tgcggaactg
     7021 ggcgtgcacc gcctttcaga agttgtggat acgctgctgg tcctgcagaa aaatcaccgg
     7081 atacggtttg atatctggca ggtggttaag cgcgatcacg ccattatttc gttcttcgac
     7141 caggtgtttg atcagggact gaatccggtt gtgccctgga gtgcctactg gacccctctg
     7201 cgctatcccc tgctgctgaa tctgtctaat ctgtttgatg atgagctggc tgaaaaagcg
     7261 tggcgtgccc gtcttgaggc tcatgatgaa cgttcttgct cattattcag tgaggtttgt
     7321 ggagtgctgc ttcagcgcgt tcactctctg ggtgatgccc gttcagttga actcattacc
     7381 gatgccctat cttgggcgat ggtcaacttt gatgagctgg ggtacaactg caaaaccaat
     7441 aaggaaaagt tgcagatcat gcccaatatg attggttttc agtccgtttt gcacgggatc
     7501 tgctctcgtc tgggggcacc gaaccggaaa gcagatatca ttgttgacca gcagtcacag
     7561 ttcaacacca cccagcggga gttgaacgag ttttactacc agatccgcga acagccgtgg
     7621 gcactggggc cgggtttgcc tgtcatggat atgaagaaca tgccagcaaa accgcttgtc
     7681 ttccagtcgg gaacgatgag tgccgggctg gaactggttg atatttatct ctggatcttc
     7741 aaacgataca tggaacggaa ggaactgacc aaaccccttt cccgcctcgt ctacaccaat
     7801 cttaaaacag cgagaactga cagtgtttct ctgcagtcag ttgcaaagag gtttaaggag
     7861 ttttttgaaa aactttctga accaaccgca gaaatgatag aaaaagcaaa tgaactcagg
     7921 gccgtggaag aaacgcgccg tcttgcccat cgtgtacaaa gtgtgtcgca atcctgatca
     7981 gaaaggttaa ccgcatatga aggcagaaaa tgcccgtcag gttcaggggc tgatagaact
     8041 agagaaattt aaccctgaaa cactgtgctc gggcgagagc tggatggcac cttcagccag
     8101 tgaagtcagc gtggtccggg cgctgatccc gctcacagat atccagctgg ccaaccggct
     8161 agatgtggac gagcgcacca tccgtaagtg gaaatccggc gagaccagaa tggtgttcac
     8221 cacctggtgc tgtctgtgct ggctggccgg gttggggatg ctgctggaag aaccggctta
     8281 gccggttctg atgcgtttcc agattctttt gagaatgaag cgtcgctgat atcgccattg
     8341 cgaacggtag atatggggga gtcgatcata gttcttccag aacaaggtcc agcctttata
     8401 ggcatcaaca agtagggcaa tgagagagcc agtttttaag aggccataga aaacggccat
     8461 gagaaataca gtcatacgat gttccttatg taaaactgta tttaggaaat ccgaagcagc
     8521 ctttaaggcc ggttgatgaa aatgagctga aaaaagtttt tctggcctgt tttttgcaat
     8581 ttcaggctaa ctctgttctg aattttgccc actgagcaat aattaccata tctcaacaaa
     8641 ccaccatttt ttgtgcttat cttgcaacaa ctcttctttt tctcttctga gatccggttt
     8701 ccggcaaaat cggaaagtaa ggatatgaac cgtagggata tctgaatggt atagttagat
     8761 tttctgcttc cgaaggagga tttagtggga aactattcag actttgagac tgattttatc
     8821 gaaagaacac tggccctaat cgatcaatat aacaatatga ttgaggggaa gcccttccct
     8881 gagcagtaca actatacgct tactctgaac tgtcttttgg gcctaatcgt gatgccgaga
     8941 gagcgcgctg tttcttacct cccgtcggat cgtctaacgc cagagcttaa agctgaaatt
     9001 gggttaaacg agtcacagtt accgggtgag gaaatgaact taagagaact gattcataaa
     9061 atgcgaaatt ctgtggcgca tttctgtgtt caggttgaat ctatcagcga tgcacgccta
     9121 gttgatcaaa tcatcttcaa agagacgcat ggtgctggcc gggcttatgc aattttcagt
     9181 gccccggagc ttctcccatt tctaaaatat tacgcggctt tgttgatcgc caatatgaga
     9241 cgccatcggg gtgtccccac gactgacgtt gtgtaaggaa tgcaaccatg tttactctcg
     9301 attgttcaac acgatctcag gcgctgcttt cactttcatc cggttttggt tgtagtgtaa
     9361 tggagctaaa aaaggttttg ttatctctcg atcttgagca gatttacgag acggatcatt
     9421 ccattatgat cgattcacga cagtatctga gagagtacgt atgtagggaa ctgggtattc
     9481 cgggtgagtt caccaccgca tactggtttc atggtacccg cacttctgct gataatattt
     9541 ttgaaaatgg tttacttgca ctgaaccaaa ctgagtcact ggtgatggac atgctggtaa
     9601 atctggctcc agacgctgag gtcaaagaga aattacaggc ctggaatttt catgcaggcg
     9661 tacctgatca cctgtttcgg acccgaacca gggataaaat gcactgggga ccatatggtc
     9721 accttgtgcg ggaggtccat cttcatgcac gtaaactctg gcagcacgac tatgtacggc
     9781 tgccggagct ggtagaggat gtctgcaatg cttacaaaaa aaaatatggg caggatctta
     9841 caggacatta tcttgaggta ctgaaacctt gcatagtctg tttcagggcg gatattgatt
     9901 atgaaaaggg tgcgcttgaa gctgcgctta gttacgcata cacgtctgtt agagaattgc
     9961 caccggatag tggcgctgtg ttcggtattg atcgacatgg taaatccgtt tctgtcgatg
    10021 aaattgtgaa tgttgaattc atatgagctt gatgactgaa gcccgtaggg ttatagatgt
    10081 tatcaatact tcttttttta gtggcaaaat tttctacagg aaaatgaagt ttgtgacaga
    10141 taatctaaag ggcctcattt atgtcattta acctattgta tttacaggtc taaactgcag
    10201 gctttgtcgt ttcgatgctt gagtgtattg ttattattaa atacgcagtg tgacaagtga
    10261 gcaaaagatg acatcgatta tagttacttc gttactcaaa accggtcctt gccttagctc
    10321 tgtacttgta gaggaaatgc tgaaaacctc gggcgttaac cgtgataccg cgcgcaaaca
    10381 gatttcccgg gctgcttctg cagggcaaat ccactgtgtt gacaagcttt tccccaagcg
    10441 cgaacgattt atttatctga aacaagagta tggtaccggg cgcttctggt ccagtctcaa
    10501 tgcggccctt cttgataccg ggtcggccta cggtctcgcc ctgagttgcc tccgggcacg
    10561 cggggggatc ctgccggtca gaaatttttc tgcagcctgc ggttcgccgg tagcaatgaa
    10621 aaacagatta tcctggaagt cagttctgga cggtctgctt cagtacaaaa tggtcagggt
    10681 cgttacgtta ccgggtctgg gggaatgtgt tgcgctgaca gaaaaaaatg ataacggtta
    10741 tctgcgcgcg ctccatcccc tgaaagccag actactcacc gagtcagttc tgatgaaatc
    10801 cctgagtcag tgggtccgta acaatggcat catcagttac gacacgctgc gtacgcgaga
    10861 ggaactcaat tcggatcaga ctccctgcgt ggcaaatttt gattttgacg tcactgctgc
    10921 atcctatctt aatcctcttc tgcagttctc ccggtcaggt gaaatccggc ctggtttctt
    10981 tgtctgcgac atgctcttgg gctgtaagct cagtctggtg catctccagc cttttattac
    11041 gaaatgtcgc tcgataaaca gcctccggaa cagccccaga tgcctgttta tgttcatcgc
    11101 tgatgaatac agtgaagagg catttctgga aatgaagcgt gccgggataa tacccgccac
    11161 gccggaaaat ctcttcggga aagactttgc tgatgcactc tttcagctac gggatcttgt
    11221 cggctccata acgctcagct taaaagataa tatagccgca atagatgaca tcatgtccaa
    11281 gctggcgaat atagccggcg cgactaatca gcttcagggc gatttatttg aatatattgt
    11341 cgctgagacc gtgcgtatag acagtaagga cgttgaggtt ggaaaaatat gtaagtcgct
    11401 taagggggag acggcggaat gtgatgtact cagtttgaat gggcatgcga aaattacctt
    11461 cattgagtgc aagggttata aaccctattc gacggtgaga catgaagatg taaaaaaatg
    11521 gattggtaag caggttccgg tattcttcag ctatgccaaa agggaatacc ccaacgctga
    11581 aattaatgtt cagttatgga ctacaggaaa gctctgcgat gattccagag agtcgcttcg
    11641 taaattccaa gaaaataact taactaatca gcgttataat ataaccgtca tggagccgca
    11701 tgaagtttgt gccagaataa aggcgactag gaatgacgcg cttatccggg tttttgataa
    11761 gcattttctc agttatccag aaaaaatcgt tcgccgtaaa catgtgcccg acccggtcag
    11821 gcttgcaggt catgatgaag caatagaatt tgatttctga accggtcggc tcgtttaatc
    11881 agagagaaaa cgttatggat tacacaaaaa atcagcatgt gctttcacag tgggtgttac
    11941 gcaactttcg cagcgatgac acagccctgt atgcaaagga aaagcagcgg gtatggtgtc
    12001 ataccgtgta catgacgccc gaaaaagaaa atgttctcca cacgcaacct ctgcctatat
    12061 ccagtgtggc agtatcgaaa aactgctttc gtcttattga tgctgagaca ggacaacctt
    12121 tcgatattga agacgaactg ggtgtctacg agcgcctgct cgctgggatc gtgaacgata
    12181 tcattcatga gcacaatttc tcacgccttc gtcacacaca tcccgatgat ttcccggtag
    12241 agaagctcac cagcttcgcg gtgatgcagt taatgctaaa tctgcacaat ccgcaaaata
    12301 aaaatccgta taagcaagaa atgcttgaac agttccatgc cgatatacag gaccatttat
    12361 ccgaatacat ccattctata aatcagttgc cccacagtaa cccagagtta atggatgatc
    12421 atttctatcg taaaatactg cgtgttgcaa actcagcctc ttccgatgag aataaatgca
    12481 gagcactttt tgtgttattt ggccttttat ccaccctgaa taaaccgacc ctttacgata
    12541 aaatcaacaa attaaggaat aatattttta ccggtatcca taccattgag gtcattcata
    12601 caggtcatga tttcgacagc actgaaccga gacctgcatt cgcaatagca cctaatattt
    12661 tttgcattta taaagatgaa aacagaattt atcttccgct atctcataac ataaccctgt
    12721 gcttcattac tggcactgca ttacatttca gaccccgcat agatgtcttc tctcctcgtc
    12781 ccgagcggct gaaatgttgc cacgaccgga gtcttaattt ttataatgtt tcgtttgatt
    12841 acattgacaa tgtgatgaca actattgata tgtataatgt gggtacatcc agcacatttt
    12901 attcagccta tgagttgagc aaacttaatg aatatctgga taagcagcag caggatcatg
    12961 actattatta tacgccggaa aatcctgtta aatggcagcc tgcgcagacg gtcacctaat
    13021 gaaagccgtt aacgaaaatg atttccgcga gctgattgag gctaaacaat ttaatggcta
    13081 tacgctggtc agcggagaag actggcagat acccacggcc cacgaaatcc tgctggtcag
    13141 gggccttatc cccctgactg acatccagct ggccaaccgc ctggatgtgg acgaacgcac
    13201 tatccgcaaa tggaaaaccg gacagacccg gatggtgttc accacctggt gctgtctgtg
    13261 ctggctggcc ggactgggga tgccgctgga taaccatctc agcggttaaa gagacgcttc
    13321 cagaggcgtt taagaatgaa tcgtctctgg tatcgccagc gtgagcggta gatatgggga
    13381 aggtcgtcat agttcttgaa gaaccaggtc caacctatat aggcatttct cactaagaga
    13441 atgagagatc ccgctgtcaa aatgccataa cccatggcga ggataaatac agtcatacaa
    13501 tgttccttat gtaaaactgt atttaggaaa tgcaatgcgg cctttaaggc cggttgacaa
    13561 aaaaacctga aaaagttttc ctttcctatt tgttgcaatt taagtctcag tcagtcctga
    13621 attttgcatt tcttgcaaca atccccacgt agcaacaatt cttaactttt tgtgattgtc
    13681 aggcaataat ccctctcttc tgcctttgag atccgttttc tgctataacc ggagatttaa
    13741 atttcatcag tactgacagg gtgttaaaca caggtattat cgaacggttc tgttttctaa
    13801 tgttatgccg caaaccagct gcccatgccg tcaatacaga tgcaccggtg cgcctgcgtg
    13861 gcttttcagg gagataaagt tatggccgat tcagtcaatc tgcagaccga tgtggtcaga
    13921 cagcatacgg ttccccgttt tttactgaag catttcagta cgccgggaaa gggtaagcgt
    13981 cagcgtttat acgcctttga caaggctgca gggcgcgcct atgccaccac gccggatgat
    14041 gcaaccgtcc gcaacacgtt ctacaacctc gaaaatcacc cggaccgtct gagtcttgaa
    14101 ccactgctgg ggatttacga gcaccacgcg gcaccggtaa ttgcagccct gctggcgcac
    14161 agggatattc gcaggctgac agatgacgaa cgctacaggc tggccgtgtt tgttgccgtg
    14221 cagcgcgcac gaaccttcgg cgaactggag cgtatctcag gcatgatttc ggtgctcacc
    14281 gataagatgg aggcaatcgg ctcgactaaa gagcaggccc tggaaaccct gggcctgtcg
    14341 tccgggggcg ataccaagga tatttttctg cggcagctcg tccagcagat gtcacatatt
    14401 gatcacctgc tgaaaaagga ctggtacctt ctggaaacca ggccagaaag tccgttttac
    14461 gtatcagaca atcccgtcgt gcttaagaac agcaatgact tcggtccgta cggtaatctt
    14521 ggtctggccg ttcgcgggat acagatttac ctccccctgt catcgacgct gatgctggcc
    14581 atgtattgcc cctccatccg tgaacagatg attcgtcaaa agcagcatct tcacaatctg
    14641 cttgccagag cgccgcatct tatcccccgt cacattcggc cttttgaaag actggaacat
    14701 atccggaggt atacggacta tcttttcatg ccgcttaccc cggatcatgt cacgcattac
    14761 aacgccctgc aggtggagtt cgcggagcag tacgtgttct gcggtgaaaa ggatttctct
    14821 ctggttgaga gaatgctggc cgatagtgaa cgctaccgga caggcccgcg gtttacgttt
    14881 tagttccaat tccggcctca cctgtgccgg aaaatgacgg cgggagcccc tgttgtacag
    14941 aggagttttg ccgtgttaca cccacgcgaa agcggcagtg gccacaggga ccaccgccgt
    15001 aaggagatgg tgaatgcctg ttgacattca ccatccgttg gtgtagcttg ctgctcttac
    15061 tttaatagag taaactttcg gcagatggcc cccaccatct taccgatacc cctgagcaac
    15121 aaactcttta ctggcgtgac ccagagtgct gaaaccgcaa atttcgacac aataaaggcc
    15181 ttaagccagg gcactacagc ccagaccacc gcagcacaga gacccacgta ggccataaga
    15241 agcaaaatac agagacaggc gctccagtat tgcttctgtc cgatgtgccc acaggcactg
    15301 acgtaaaggg ttttagctgt ggtgtaaatc gtccagataa aatggatttc agtctggaat
    15361 gggaatagaa ccgttccctc ggtgtcgaac aaatttttca taaagccctc ctgggcaggt
    15421 tgttgttaac actgatgtgt taatcttgat catcttcttg acctcttttt tcagataaaa
    15481 ggcacccggc gcctcacaca cgtgaacgct gttgataccg tacagaatgt tttctctttc
    15541 aattctgcta tgatgtgttc attcgcatcc tcctgtctgt cataccgatt ttaatctcgt
    15601 cccgggtgct ccccggaaaa aaatccaaaa aaatccgggc cggaatggtg ataaccggtc
    15661 tggcgggaaa taaacggatt tgttggagtg gagcgggacg gaacaatact atgaaagtaa
    15721 tgttaacaga aaaccacacc attgtcaagg cgtagctacc cattaaagta actatagcat
    15781 gctattgttg atattccttt agcaaaaagt gctatccatc ccgtcgctgt atcttcattt
    15841 tcgcccggag cggggcagct aggcgaatac ctttttcagg tttttgcccc atgccccctc
    15901 agagtaccga cacaaacaga tgggtggcca cgccctgccg gcgcccgctg aacggccctt
    15961 cagatgttcc ggctgacagc tggcattttc gtccgcaacg gcagaaggcc gacttgaggg
    16021 atttggacac ctcccgcagc gcgtcttcca gatcggtgtt caggctgagt atcaccactg
    16081 tcccggccgg gcgacgggcg atctccgcga aactctcccc accggtaaca tagcggctct
    16141 gtcgaccttt cattatgcct cctgatgatg cagttgtggt tgtgcccgta cacccgggtc
    16201 cgttacagta gatcgtgcac caccgcttca atcagcgcct cagcgacggc cggcagcggg
    16261 gtcggattct gatcttcttc cacgccattg ctataaagat gaagctcaca aatcccgtcc
    16321 acaaaacgga aggccttatc ccagctgtcg ggagcggtgc tgatcagggc gtcatagccc
    16381 gggtcagtgg taaagcgacg tccgtcaagt tccgggaacg ggatgttcca cgtctgggta
    16441 atgacacttt catccagtac cagcagcacc cgccaggcac cgtagccgta gtcatccggg
    16501 cggtaaatac gcacggtgat gtcatccctg gtccagctgt agccgcagac ggcatgctgt
    16561 ttcttcaggg cacgaataac atcaatatcc cggtaaaagc ggtgcttttt ctccctcagc
    16621 tgtgagtaga aaatcctgtt ccaggcactg tcaatggttt ccctgtcccg gccggcgatc
    16681 tcggcaaaca gggaaaaggt cagggagatc ccgcgcggga tctttttcca gcccttaagc
    16741 acctggtcgg cacgtgcgtg caggaagggc gtgttggtca gcaccacccg gcgaagctcc
    16801 tcttccgtcg agatcccgag cagcttctgg tattcctggt tccagcgggt gatgtaatgg
    16861 ctctggtagc gtttctccat gacctcggcg cccagataac tggccagctc gtcatcgaga
    16921 gaggcgatga gcgggttttt cagggcgaaa ccgtcgatga cgtcccggaa gctgtcttcc
    16981 aggaactcac gcagaaacgt cgcctttggc ttacgggcgt gagttgccag gtcggtgatc
    17041 acccggtcga tgtcggcggg aatgttccgc agagtgtacg ttacgctctc tccaccgtcg
    17101 tttgtcatgt catgttcttt ttcactcatt ccgtgtacct tttcatgtgt ggatgcatta
    17161 ctgcaatact catacactat cctgggttta agactattta aatactgcga agcatatgtt
    17221 tttataattc gtatgctttg atgtttattt tctgttcacc ttcttttctg agatccgaaa
    17281 actcccggtt acggatcttc ctccccctcc gcacagcgcc acaccctgta agcacaacat
    17341 gtagtgctga attcagctgc tcttgacact atatgttgtg tcatctccct gacctgtgat
    17401 gcgccgcgca ggggcggaaa acagcgatat gattatttcc tcagcgtggt acacttccgg
    17461 aaagttgtga tattcaggaa agtcggatct gacgaaaacg gctctccggt aatttaacgg
    17521 cgtggttatg tggctgctta ttatcagcct tgaaatgatg acgtcatggg aaaatcatcc
    17581 gtgtgacaga agcagggttg tgctgcgcgt catcattact ctgtgtgact attgaaccgg
    17641 atactatttt tgaggcgagg tatgggtaaa ccaacattta gatcatttta cgatgtggtc
    17701 cgggagcttg aggatgtata tggccataaa gagctatggc tgtattcagg agcggcatac
    17761 gcaacgccaa cagaaatgat taacgccaga cataactgga aatcgccaaa aatcctgaaa
    17821 cgtaacggaa gaatggttgc agaacgaatg gataactcgg actcatggca actggttggt
    17881 gactacaaaa aaccgctgtt ccaacattgt gctccgccct ggcaatcctg tcagatagat
    17941 gactatttta aaggctatta cattatcgcc ccctgaaggt cagaggagta attgatggcg
    18001 ggattacatc attgatggcg aggtttaaat gggaagtatg aaagaactgc tgtttgaaat
    18061 gcaggaggaa cggcgtgacg aatggattgc ggaaaattat ccggatgctg aggaaggaac
    18121 tcctgaatgg gatgcggcag cgcaggaata cagctggttt caggactgga tggaagaggc
    18181 ggctgagcag caatattttg aggcttcgct tgccagtatt cctgaccgcc tgcaggatgc
    18241 aaaggctgaa ctggatgagt tagagagcct gatgcagttc aaccaaccca ggatcgtaga
    18301 gcggatggct tacgttcatt gcgtgagtgt actggacagc tttctgatgt acagcgcaag
    18361 agcacttctt agtcatcccc cgcatttaca gaagttcctg cacgaggctg attcgttggt
    18421 tcctaataag gaagacagaa gaaaattact tgcttccaaa tgggtcgagc aggagccgga
    18481 taaagacact cctgagaagg tttatacctg gcgagctcag tctcttgtag cgaagaagac
    18541 atttcagagc cacaaagtga taggttggta tttttcaagg atgctgacaa cgccacatga
    18601 gtggccattg gaggagatta agggtgtaat caagatccga aacgcactgg tacaccgtaa
    18661 tggagttacc gaatctcttg aacccgtgta tatcagttcg gggagtgtgc agaatgctat
    18721 ctgcacggta cgagcattta ttaccgtggc ggctgaaacc cttctgcagg aagatgcact
    18781 atacaggacg gatgatggca tattctgaaa ttaccgcagc gacaccatcc ataagcgtct
    18841 tcactgaggg atatgtcgat gacttcgctt cccgctaact gtataccagt aagcaacaca
    18901 tcattacctg tcgccataga ctacccagca gcgctggcac ttcgccagat ggctctcgtt
    18961 caggatgaac tcccgaaata cctgctggcg ccggaagtca gcgccctgct ccactacgtg
    19021 ccggatcttc accgcaagat gctgctggcg acgctctgga acacgggcgc gcgaattaac
    19081 gaagcgctgg cgctgacaag gggggatttt tcgcttgcgc ctccgtatcc gtttgtgcag
    19141 cttgcgaccc tgaaacaacg gacggaaaaa gccgccagga cggcaggaag aatgcccgcc
    19201 ggtcagcaga ctcaccggct ggttccgctc tccgacgcct ggttcgtcag ccagctgcag
    19261 acgatggtgg ccacactgaa aatccccatg gaacggcgta ataaacgaac aggcaggaca
    19321 gagaaagcgc ggatctggga agtgacggac agaacggtca ggacctggat tgggcaggcg
    19381 gttgccgccg ctgccgctga cggtgtgacg ttctctgtcc cggtcacgcc acatacgttc
    19441 cgccattcct atgcgatgca catgctgtat gccggtatac cgctgaaggt cctgcagagc
    19501 ctgatggggc ataagtccat cagctcaaca gaggtctaca cgaaggtgtt tgcgctggat
    19561 gtggctgcac ggcaccgggt gcagttttcg atgcctgagt ccgatgctgt cacaatgctg
    19621 aaaaacagac acgcataagt cacaaatcat aattatgaat tgtgatttat tctataaaag
    19681 aagagaccac tgcaatatgt gatctcttgt atgcaagggt gcttaaacag tgtgaattca
    19741 caagtgtgat ttcataagta ataacttctt gattattacg ttagtgtttt taagtattgc
    19801 tcaagggcta ctctgacgat ggcacttaca ttttttggat ctttaccatt gcgtttattc
    19861 ctcaatgcca gatcttctat tgtgacaagc atgctttcac caagagagat tgtcgtccga
    19921 cattgttttt ctggttcggg tttttccggc gcgccgtatg gtttatccgc aaggcgctga
    19981 gccagagcct ctgcttgttc taccgtaact gcagctctgt ttaatgcttg ctggctgggt
    20041 ttcttcacca tgggccaaat acctcattca ttaaatgttc aatttctgct ctggctgctg
    20101 tattgttcgt ttctactaca ccggtgccgt tgctcatgca atcgcgatag acttttctga
    20161 aacaaataac tgaatccagg acttgaattg ttgggaattc ttcaagatat tcaaggaatt
    20221 cttttctctc atttcccctc aaaaccggat tggttgttgc catactttgt aaacaataaa
    20281 ccttaagttc cgggttaagg ttacgcattg cgtctatctg ttgttctagt tctgtaagag
    20341 tatccagatc tggttgagaa cactgtagtg gcgcaattat ttgatgtgcg acaactccac
    20401 tcgttatgaa ttcctttgag tttcttcctg cgacatccac tattacatca tcgaactttt
    20461 cattcagagc ctgtagtgtt tgtgtcaggt tatcaaattt ctcaacaagg gttattgctg
    20521 gtaacagtcc tgcagcttct cgttctgcat gatgttttgc ggctgttcgt tgtagatctc
    20581 cgtttaacag gcagacttcc cttcctttat ttgcgagagc aatagccagg tttgatgcaa
    20641 tggtagtttt ccctgaacca cctttgttcc caccaactac agtaatcatt gcaacctcat
    20701 aaatgtgatg tgtgaagtat gatgatattt tgacacggta acctgatgtg tcaacatgca
    20761 ttcatatttg tgaagtatga aatatgaagt atgtcatatg atgtaataaa tattacttgt
    20821 gttttgtgat ttcataacta tgatagtgct caaattgagt attaccttgc aaccagaaac
    20881 agtgatttac tcagattgag tatttaaggg gctatagatg aacgtatact gcgatgatgg
    20941 ttcaacaaca atcaagttgg catggaatga caatgggaaa atctgtaagt cgttgtcgca
    21001 aaactcattt cgtcatggat ggaaggttga cgggttaggg attcgccaga catttaacta
    21061 tgaactggat ggtaaaaaat acacgtatga tgaagttagc aaccaatcta ttcttacgac
    21121 tcatattgag tatcaatata cggatgttaa cctgttagct gtgcatcacg ctttactgaa
    21181 tagtggatta gcgcctcagc ctgtatcatt gactgtgact ttgcctatca gtgagttcta
    21241 cacgaaagag tgtcaaaaaa atgaactcaa cattcagcgt aaaattgaga atttaatgag
    21301 gccgattcgt cttaataaag gcgatgtttt caccattgaa cacgttgatg tgatgcctga
    21361 atcattgcca gctgtgtttt ctcgtctggt catggataaa gttggtcagt tcgaaaaatc
    21421 gctggtagtt gatattggcg gcactacgct tgatgttggt gttatcgttg gtcaatttga
    21481 ctctgtaagt gccattcatg gtaattctgg cattggtgtg tcttcagtta cgaaagctgc
    21541 aatgagtgca ttgcgcatgg catccagtga taccagtttc ctcgttgcag atgaattgat
    21601 taaacgtcgt aatgacccgg actttgttcg gcaggttata aatgacgaaa caaaaactga
    21661 tctggtcctt aataccattg agggggctat tgccagtctg ggggagcaag tagtcaatga
    21721 gctcggtgat tttcatcatg tgaatcgtgt ttatgttgtc ggtggtgggg ccccgttgat
    21781 ctatgattcg ataaaaacgg catggcacca tcttgggcaa aaagtagtga tgatggagtc
    21841 gccacagaca gcactggttg aagctatcgc ggctttcaaa gaggagtaat attgtggacg
    21901 atgaacggaa aaggaaaaaa ttcacacttt atctgcatcc ggaaaaagct gcagactttc
    21961 aaactttgga agcgatagag tccgttccgc gttctgagcg tggtgagctg ttccggaatg
    22021 catttatttc tggaatggcg ttacaccaac ttgatcctcg cctgcctgta ttgctgactg
    22081 ccattttgag cgaggagttt tcagcagatc aggttgtaac cttactcagt cagacgacag
    22141 gatggaagcc ttctcaggcc gatatcagag ctgtgttgac tgaactcggt gctttgcaat
    22201 ctgctgaaaa aatgcctcct tctgctactg attcggtgca ggaggcaatg aatgatgtgc
    22261 gccttaaaat gcaaaaatta ttctgatata tcagaatgaa gccatgggga tctcagtcca
    22321 ttgtgttgta tacgaaggcg agagcatttc ccgtttcatt tgccattcag gggcggttcc
    22381 tcgccccgca aaccacactt tcccttttcc agactggttc agttcatcga gtgttttcat
    22441 taacttttcg ctgtttttac ggggctggat ctcatcaaat aatcccggct gcgctatacc
    22501 cgatggtgtg aaatcagcca gcatgactcc tgccttcata tagcggtacc cttcacgcca
    22561 gacatggttt aacgctctgc atgctgcggc aataatgtcc cggctgtcct gtgtgggtaa
    22621 tggaagcttt tccacagcgg cattgctgta acagggttct tttactgcaa agggggatgt
    22681 ccgtacaaat gtcgtcacct gccggcaata ctgacgctcc ccacgtagtt tctctgcggc
    22741 ccgctctgca tactgaacaa cagcctggtg catggcatct ttgtctgtga ttcgttcacc
    22801 aaaactgcga ctacagacaa tctgctgttt tgccggtggt gcttcttcca gggatatgca
    22861 ggactcgccg ttgagttcgc gtaccgtacg ctcaagaatg acgctgaagt ttttccggat
    22921 gaatgccgtg ttagcctgcg ccagctgcag tgctgtgtta atacccagcg cattcagctt
    22981 ttccgtcagt ctgcgtccta ctccccagac ctcaccaact ggctgcagcc ccagtagctt
    23041 caagatccga ttacggtttt ctgccgtcag cgcgaccaca ccggaaaact gtggccattg
    23101 ctttgttgcc cactgtgcac ttttagccag cgtttttgta ggcgcaatgc ccacccccat
    23161 ggtgagtcct gtccagcttt ttacctgttc cctgagctga tgaccaaaaa actccggaga
    23221 gatgcaatga tttatccccc gcaaatcaat gaacatttca tcaattgagt agggctcaac
    23281 tgcgggagaa agcgactcca gaacagccat aacccgttgg ctcatgctgt ggtacagcgc
    23341 ataattgctg gaaaatacat gtattttctt ctccaggcgc atctgtctca cctgaaacca
    23401 gggctgcccc attctgatgc caagggcttt tgcctccggg ctgcgcgcga tcacacagcc
    23461 atcgttattg ctgagtacga tgactggttc gttgcgaagg tccgggcgga aaactttttc
    23521 acatgaggcg tagaaactgt tgatatcagc cagtgcaaac atcagcgtaa ctcccgcgtc
    23581 ctgtgtatca cgtgagtgac aacaccaaaa atacagatgt tttccggata cagtgtgcgg
    23641 aactccgggc tgtctgaaac cggctccagt gccgggcgtg ggcgcaacag cagtcgtttg
    23701 acggtgaact caccgtcgat ctcagcgata acgatgtccc cgtgttgtgg tttttcggcc
    23761 ctgtccacta ccagcagatc accattctgc acgccagcct ggttcatcga ttcaccgctg
    23821 gcgcgcagaa agaaggtggc tgcaggtctg ctgatgcaat agctgttcag atccagttcc
    23881 tgctcagcat aatcagtggc aggcgaagga aaaccggcct ggcaacgatc ggcaaacaac
    23941 ggtcgcacgt aactgtcatc gcctgaaggg tcagccggac ggtgataaac ggtactcatg
    24001 ctgcaggcag ccagctgtcg tcctgccaga tttcttccag cagagacagg atacgttctt
    24061 tatccttgtc gttgcgggct cctgtgacag atacagacgg ggcgctgccc tggtctatac
    24121 gcagccagat ttccgggtag tgtggtttca gacgttgttc gatttcattc tgtagcgcct
    24181 gaagtgtgcc tgatttgagg ttttttgtgc tctggcggtc gaaaagaatt tcaatgcgga
    24241 tcatgttctc atctcccttt tcttttgctg tatgcatata cagtattcta ataactgaat
    24301 ccacacacag tcaaatgtca ttattgtgac taaaaagcgc tcaattctct gagagtgtgg
    24361 ctgtatgcct gctgtggact gctgtgttca gcttatccac agcattttgc gcacggttct
    24421 gtgaataaaa tacctggtta cccaggccgt gccggcacgt taaccgggct gcatccgatg
    24481 caagtgtgtc gctgtcgccg gcctcctcac ccggtcacgt ttcgtcgttc ctcctccacg
    24541 cgctgcggct tcggggccgc acctgcattc gtatgcggtc gcccggttac aggtgcggca
    24601 cggcctgatg gaggccgcat gtgagaggag aattcccatg ccaaactggt gctcgaatcg
    24661 tatgtatttt tctggtgaac cggcacagat cgctgagatt aaacgactgg ccagcggcgc
    24721 agtcacaccg ttttatcgcc gcgccacaaa tgaaggtatt cagctgtttc tggccggaag
    24781 tgccggactt ctgcagatca ctgaaaatat ccgctctgag cagtgccccg gagtgacggc
    24841 tgccggacgt ggtgctgtat cgccggaaaa tatcgcgttc acctgctggc tgacacacct
    24901 gcagaacggt gtactgctgg atgagcaaaa ctgcctgatg ctgcatgaac tctggctgca
    24961 gagcggtact ggccggcgtc gctgggaagg gttaccggat gatgtcaggg aaaccatcac
    25021 tgtgcatttc accgcaaaaa gaggtgactg gtgtgacatc tggggtaacg aggatgtatc
    25081 ggtgtggtgg aatcgtctgt gtgacaacgt actgccggaa aaaaccatgc cgtttgacct
    25141 gctgacggtt ttgccgaccc gcctggatat tgaagtgaat ggctttaacg gcggtgttct
    25201 gaacggtgtt ccttctgcat atcactggta tacagaacgg tatggcgtga agtggccttg
    25261 tgggtatgac ctgaatatca gcagtcaggg ggaaaacttc attcaggtgg atttcgacac
    25321 gccgtggtgt cagccggaaa gcgacgttgt tgcagaatta agccgccgtt tcagctgcac
    25381 gctggagcac tggtatgccg aacagggctg tgatttctgt ggctggcagt tgtatgagcg
    25441 cggagagctc gttgatgtgc tgtgggggga actggaatgg tcttccccga cagatgacga
    25501 tgagctgccg gaagtcaccg gacctgcgtg gatagtcgac aatgtggcgc attatggcgg
    25561 atgaagtatg acggagacgg gcgggaaacc gcccgtttct tttccgacaa aggatgtcgc
    25621 cgtgctcctc tttttactgc gccggctgac gcggcgcggc ataaacgctg cctgtggtca
    25681 gtgtcccgcg tccggcgggc acgggacggg cgcttttacc gctcccggct ggcgtcgatg
    25741 acagtgtacg ccagcctgtc gcccttttct gatgaacgcc cttcaagccg ctttcgcggc
    25801 atattctcgc cgtcaaaaag acgacggctg cgatattcca cggtcggcct gacccgttac
    25861 cggacgcggt gaacagccca caggcagcgg ggaacgggca ccacaggggt gccctcccgg
    25921 tgccctctgt aagagaagga gcgttgttat gtcccgtttt gtccttggta actgcatcga
    25981 tgttatgacg cgtatccctg ataacgccat tgatttcata ttaactgacc cgccatatct
    26041 cgtcggtttt cgtgaccgtt ccgggcgcac catcgccggc gataaaaccg atgaatggct
    26101 gcaaccggcc tgtaatgaaa tgtaccgcgt actcaaaaaa gacgcgctga tggtgagctt
    26161 ctacggctgg aaccgcgtcg atcgctttat ggccgcctgg aaaaatgcgg gattcagcgt
    26221 ggttggtcac ctggtcttca ccaaaaacta cacatcgaag gccgcatatg tgggctatcg
    26281 ccacgaatgc gcctacatcc tggcaaaagg ccgtccacgt ctgccacaaa acccgctgcc
    26341 ggacgtgctg ggctggaaat attcgggcaa tcgtcatcac ccgacggaaa agcccgttac
    26401 cagcctgcaa ccgctcattg agagcttcac acacccgaac gcaattgtgc tggacccgtt
    26461 tgcaggcagc ggctcaacct gcgtcgccgc cctccagtcc ggacgccggt atatcggtat
    26521 cgagctgctt gagcagtatc accgtgccgg gcagcaacgc cttgccgccg tgcaacgggc
    26581 catgcagcag ggggccgcga atgatgactg gtttatgccg gaggctgcgt aaatgaacta
    26641 tgccggacac gaaaaactgc gcgccgaagt ggcggaggtg gccaatgcca tgtgcgacct
    26701 gcgtacaacc atgaatgaga tggagcagcg gtacagcttt aatgccgaca ccctgccgga
    26761 acgtctggtg cgtcagacgc tgtttcgcgc aaaccgcctc ctgatggagg catataccga
    26821 aattctggaa ctggatgcgt gctttaaaga ctgagaagga gacgagaatg tacggaacat
    26881 gcgaaacgct ctgccgggag ctggcagcaa agtatccggg agacacgccg ctgatgctgg
    26941 ttatctggtc tccggaagag attcaggcac tggctgacgg tatggaaatt tctctgaccg
    27001 atcatgaaat caggaccgtc ctggcgcgca tggaggacat cccggaagac cagaggactg
    27061 aatccggtat ttcttccgcc gcggtgatgg agattatcag caacgtgagc gaaaaccgcc
    27121 aggtgaccgt ccctgctgaa ctgctggcgt ccctgattca gaccgctgaa caggcactgt
    27181 ggaaacgtga gtgggccgcc cgtgataacg gcctcgtcgt cccggaatgc gtcacccgcc
    27241 gacaggcggt ggttaatcag gcccgcgccc tgctgaaaaa caacacacgc gaaaacgact
    27301 gatgttatcg ccgcctgccc tgcgggcggc gatacaggga gaacgcttaa tcatgaacaa
    27361 aacgctgaat gcactggttt gtcgtcacgc ccgtaacctg ctgctggcgc agggctggcc
    27421 ggaagagacg gatgttgacc agcgaaatcc gaactatccg ggctggatca gcatttacgt
    27481 gcggctggat gcgccccggc tggcgacgtt acttatcaac cgtcacggcg gcgtactgcc
    27541 accgctccag gcctccgcca ttcagagact gaccggaacc ggggcggaac tggtactgtc
    27601 cggcagtcag tggcagtcgc tgccggtact tccggcagac ggaacgcagg tgtctttccc
    27661 gtatgccgga gaatggctga ccgaagacga aatcagggct gttcttgatg cggtgcacga
    27721 tgcggtacga agcatctgtt accaggtggc agaagatgcg cggcgtatcc gtgcggcgct
    27781 gaccaccacc ggtcagacgt tgctgacccg ccagacgcgc cgctttcgcc tggtcgtgaa
    27841 ggaaagcgat cacccctgct ggctcgatga agatgacgaa aacctgcccg tggtgctcga
    27901 tgccatcgtg aaccggggag cacgtttttc gtcggtggaa atgtacctgg tcagcgattg
    27961 tattgagcat atcctgtcca gtgggctggc ctgcgatgtg ctgcgtatac ccgatgaacc
    28021 gccccgccgc tggtttgacc gtggtgttct gcgggaggtg gtccgggaag cccggaccga
    28081 aatccgcagc atggcggatg ccctggcaaa aatccggaaa tgatgacact ggcggggtaa
    28141 atccccgcct ttttcctgcc ggtccccgga cagagcacaa aggaagtcgc cgccggctcc
    28201 gctttacccg gccatgcggg gcatggcctt gtggtgtttc agttatgtgg cctcagcgtc
    28261 gtgtgcgggc tgtgccgtgc ctccatctta gccgggctgg cgttgatgca agggtacgct
    28321 tcgccgctgc ggtcacccgg tccctccttc ccgtctgccg tgattttccg gtcgttcacc
    28381 cgctcagatt tcgcggtctc accctccaac tcccgccagc cgtgtgcgtc aggctgcggc
    28441 ttcccttgcg ccctgtgcat ccccgcctta tcgcccggct tttatggagg cacggcaccg
    28501 cccggtgtcg cggaatatgt gaacaacgga ggaaaacatc atgcaatatg cgaaacctgt
    28561 cactctgaat gttgaagaat gcgaccgcct cttctttctg ccttacctgt ttggccagga
    28621 ttttctgtat gccgaagcgt ctgtgtacgc gctggcgaaa aaaatgatgc cggaatacga
    28681 aggcggattc tggcacttca tccgcctgcc ggacggtggc ggctacatga tgccggatgg
    28741 cgaccgtttc cacatggtga acggtgcaaa ctggtttgac cgtaccgtga gtgctgatgc
    28801 cgcaggcatc atccttacct cccttgtgat taaccgccag ttgtggctgt accacgacag
    28861 cggggatgca ggactgaccc agctttaccg gatgcgcgat gcgcagttgt ggcgtcacat
    28921 cgaatttcac cccgaatgca acgcgattta cgcggcgctg gactgattaa cggacggggc
    28981 ggcaatgccg cccctgtaaa ggagacgaga acatgtactg tactgttaaa gaaatcatcc
    29041 gcgatgtact ggatacagac gtgccggaca gtgaatgtgt ttttgccgtg gtgctgaccc
    29101 gtggggatgt gcgtcacata gcccaggact ggagtctgac agacgatgag ctggaaaccg
    29161 tcatgcagcg gctggacgat gcctttgaat atggtgcgga tgtcagcatt gttcacgacg
    29221 ttgttcgtga actgatggaa gaaaagcgcg ccagccgtca tgtgacagtc ccggcggtga
    29281 tgctggaaaa agtgatggcg ctggcaggca gtgaaatgaa gcgcctgtat gccgtcggga
    29341 gtgagaacgg gggcgacggt gacgcattcg tcagggagga acgcgaagca atggacgttg
    29401 tgttacaggc gctggacggg gagacgatgt catgaatatc agcacagaaa cccgcgaaat
    29461 tctgcgtaat tacagggccg tgattaatgc gcggcgtcgt gaaatggggc agaaaccgct
    29521 caccaccgcg cagattgttg atgaaatctg cgattttgtg gcgaatcagc aggcggtttt
    29581 cctgggcggt cactacatct tacagggcag cagaaacagg tgatacaggg gcaggcggtg
    29641 gaggccgcct gtcagcccgc gcgaaaaccc ctggcaggct gacgcctgcc ggaaacgcgg
    29701 ggtgcagcgc ctgcggcgct gccggcccgg ctttccggtc agaaggtagt gtatggctgt
    29761 atgccaggaa cagatgtgat attccgtgac actgtcaccg tttatggttg tcttttattg
    29821 cgcccacttc ggctcccggc tgcctggcgg catccggtcg tcaggcccga ctccgacagg
    29881 cagctgctgg cgcacccgcc agtctccgcc gaacccgctg gcgcggtttc gggctgccgc
    29941 tacggtccga tgcgtgaaaa cccgggcttg cagcgaccgt tacggtcgcg ccgcccctgt
    30001 cgcgccgtga cctgcgctcc ctgcacacct gacggtgtgt gaggaaactg cgcctgatgg
    30061 cgcagcagtc ctccctcctg ccgccacccc tgcggcctgt ttcccgctgt ggactttccg
    30121 gggggcagac gtaaccccct cccgttccat ctgtccaggg tgagatgcac cggccacaaa
    30181 atttttagcc atgaacccgg caaaaaattt tgcgtccgcc cccggacagc tgtcccgtga
    30241 ggcggtaaac gggcccccat cggaaaggtc cactgcgggg agaaatcccg aagaagtgga
    30301 cggcaggagg agctttcgct cctctgaagg ggcacatgaa cgtcccgtcg ccggtatccg
    30361 gtttatctca catctttcag tcaggaggtt cttatgagtg cacgtggtat caacaaggtc
    30421 atcctcgtcg ggcgtctggg caatgatccg gaagtccgtt acatccccaa cgggggcgca
    30481 gtggcaaacc tgcaggtggc cacgtcagaa acctggcgcg acaaacagac gggggaaatg
    30541 aaggaacaga cggaatggca ccgcgtggtg ctgttcggca agctcgcgga agtggcaggt
    30601 gaatatctgc gtaagggcgc gcaggtctac atcgaaggcc agcttcgcac ccgtagctgg
    30661 gaagataacg gtatcacccg ttacgtcact gaaattcttg ttaagaccac gggcaccgtg
    30721 cagatgctgg gacgtgcacc acagcagaac gctcaggcgc aaccgaagcc tcagcagaat
    30781 gggcagccac agagtgctga cgcgacgaaa aaaggtggcg cgaaaacgaa aggccgtgga
    30841 cgtaaggccg cgcagccaga gcctcagcct caacaacagc cagaacctcc ttatggcttc
    30901 gacgatgatg cgccattctg atctggctga tttttctgac agccccacca tgtgtggggc
    30961 attaccggag aacgatgatg aacaaatccg atgaaatgat tcaggaacta cccgatgggg
    31021 cattcacccg cgagtgtgct gaaaccgttg ttacacagta ccggaatgtc tttatcgagg
    31081 atgatcaggg aacgcatttt cgcctggttg tccgtcagga tggcacgttg atctggcgct
    31141 cctggaattt tgaggactgt gccgggtact ggatgaaccg gtacatcagg gatttcggca
    31201 ttcgtaagta agagaggtgc cggacgggga tgacgtccgg cagacattaa ctaaaccgta
    31261 aggggaataa tcatgtcagt agtaaagtct gaaccagaca ccacccgcaa agcaagccgt
    31321 aaatctgcaa aaacgcagga aaccgtcctg tctgccctgc tggcgcagac ggaggaagtg
    31381 agcgtgccgc tggcctcgct gattaagtca ccgctgaatg tgcgcacggt gccgtattct
    31441 gcggagtccg tcagcgaact ggcggactcc attaaaggtg tcggcctgtt acagaatctg
    31501 gttgttcatg ccctgccagg tgaccgttac ggtgtcgccg caggcggtcg ccgactggca
    31561 gcactcaaca tgctggcaga gcgcaacatc cttacggctg actggcctgt acgcgtgaag
    31621 gttattccgc aggaactggc gacggcggca tcaatgactg aaaacggtca tcgtcgggat
    31681 atgcaccctg ccgaacagat tgccggattc cgcgcaatgg cgcaggaagg caaaacacct
    31741 gcgcaaatcg gtgatttgct gggctattca ccccgccacg ttcagcgaat gctgaaactg
    31801 gcagaccttg cgcctgtcat cctcgatgcg ctggcagaag accgcatcac cacagaacac
    31861 tgtcaggcgc tggcgctgga gaacgacacc gcgcgtcagg tgcaggtgtt tgaagctgcc
    31921 tgtcagtcgg gatggggcgg taaaccggat gtgcgggtta tccgcaatct gattacggaa
    31981 agtgaagtgg cggtggcggg gaacagtaaa ttccgcttcg tgggggctga tgctttctca
    32041 cctgacgaac tgcgtactga tttgttcagc gatgacgggg acggttatgt ggaccgtgtg
    32101 gcgctcgatg ctgctctgct ggaaaaactc caggctgtcg cagagtacct tcgggaagcc
    32161 gaaggctggg agtggtgcgc cgggcgcatg gagcctgtcg gtgagtgccg tgaggatgcc
    32221 ggaacatacc gctgtctgcc ggagccggaa gcggtgctga cggaggcgga agaagaacgc
    32281 ctgaacgaac tgatgacgcg ttacgacgcg ctggaaaatc agtgtgagga atccgacctg
    32341 ctggaagcag aaatgaagct gatgcgctgc atggcgaagg tcagggcgtg gacgccggag
    32401 atacgtgccg gaagcggtgt ggtggtgtcc tggtgttatg gcaacgtatg tgtccagcgt
    32461 ggtgtgcagt tgcgcagtga ggatgacgtg gctgacgacg ctgaccgcac ggaacaggtg
    32521 caggagaaag catcagtgga ggaaatcagt ctgccactgc tgacgaaaat gtcttccgaa
    32581 cgcacgctgg cagtccaggc ggcactcatg cagcaaccgg acaaatctct ggcactgctg
    32641 gcatggacgc tctgcctgaa tgtgtttggc agcggggcgt acagtagtcc cgccagaatt
    32701 tacctggaat gtgggcatta ctcactgacc agcgctgcgc cgtcagggaa ggaaggtacc
    32761 gcattcatgg cgctgatggc agagcacttt cgccttgctg ccctgctgcc ggatgaatgg
    32821 gaacgggaca tgacgacgtt cctgtcactc agccaggagg ttctgttatc tctgctcagt
    32881 ttctgcactg catgcagcat ccacggcgtt cagacccgtg agtgtggtca cacgtcacgc
    32941 agtccgctgg actcgctgga aaccgccatc ggctttcaca tgcgcgactg gtggcagccg
    33001 acaaaagcaa acttcttcgg acacttgaaa cagccgcaga ttatcgcagc cctgaatgag
    33061 gccggactgt ccggtgccgc acgggacgcg gagaaaatga agaaaggcga tgcggctgaa
    33121 catgcagagt tccatatgaa agacaaccgc tgggtgcctg gctggatgtg tgcaccacgt
    33181 ccacagacgg atgccactga acacaccgct aacctggctg atgccgcctg atgaacaacc
    33241 acaccgcccc gccggagacg gggcggcagc aagggagata ccgtgatgaa aactgaactg
    33301 accctgaatg ccctgcagtc catgaacgca caggaatatg aagatatccg tgctgcggga
    33361 agcgatatgc gccgtaatct cactcacgag gtgatgcgtg aagtggacgc accggctaac
    33421 tggatgatga acggagagta tggcagtgag ttcgggggct ttttccccgt ccaggttcgt
    33481 ttcacgccag cccacgaacg tttccacctg gcattatgtt cgccgggaga cgtctctcag
    33541 ctctggatgc tggttctggt gaattgtggt ggacagcctt tcgccgtcgt tcaggtgcaa
    33601 catatcttca cgcctgtcgc tatcagtcac acgctggcgc ttgccgcgac acttgatgcg
    33661 caggggtaca gtgttaacga catcatccat atcctgatgg cagaaggagg tcaggcatga
    33721 gcgcacgttc acgggcactg atccccctca gcgcagagca acaggccgcc atgcaggctg
    33781 tggctgtcac cgaacaacgt cgtcgtcagg gacgcacact ttcagcatgg ccttatgcca
    33841 gcgctttctt tcgctgcctg aatggcagtc gccggatttc gctgaccgat ctccgctttt
    33901 ttgctcctgc gctgacgaag gaggaatttc atggcaaccg tctcctgtgg ctggctgccg
    33961 tggataaact gattgaaagt tttggtgaag tctgtgttct tcccctgcca tccgatgcgg
    34021 ggcatcgtct gttcccgtcc gttccttttc gtgaaggtga gcggcgtcgt cagaaaacca
    34081 cgctgacaga gcagaaatac agccgccagc gggaacgtga ggcagaacga cgggaactgg
    34141 aataccagac atgttttgct caggcgcaga ttgaccttgc gtttcatact cccgccacgg
    34201 tcggaagctg gttgtcccgc tggtctggtg ttgttgagga gcatgatctg gaaacgattt
    34261 tctgggggtg gtgcgggcgt tttccatcac tgtcatcatt tgaccggttt ttctggcagg
    34321 aggaaccact ctggcggctg atttttgaag ccggtgaggc cggtcgtggt gcaccggtac
    34381 aggtacgtgc acttgagcag tggatgatcc cgaacaagct ggagaacgca atatgatgaa
    34441 atcagacgaa caatatcagg ttcccgtgtg gatgcgacct ctgttgccgt tgctctgcaa
    34501 taccggtggt aatgatccgg aggaactgct gaatgataca gaaaccaccg ccagtgcgaa
    34561 tattgtccgt tatgtactga tagttgcagt gcggtcgcag attgatctgc tgcagctact
    34621 gtacaggaaa ggactgttgc gcacagagat accaggtggc ttttcaccgg aagaagcgca
    34681 ggaactcctg gataatctgg tgcgcagcca tatcagcaag gcgctgtcgg gtgagcgaat
    34741 ggcagcccgt gacagaaatg ccgatctgac ctggattcgc cagcaactgg tcgatgccgc
    34801 ctggtttgtc cgtgccacac tggaagcgca tgggatgggc gtcggaaatg agagtccctc
    34861 tgctccgccg gagacaatgc cggacataca gacacgggaa ctggttatgt tgatcaagcg
    34921 actggcatca tcgctgaaag cggtgaaacc cgacagtagc gtggtgcgtg aagcgcagga
    34981 ctggctgtgc gacagaaaac ttgtggatat cacagatatt ctccggtgaa ccagacatgt
    35041 atattttacg cagggacacg ccgatcatga tcgtttcata tcaggggggg tctggaggtc
    35101 atgcggcgtg tcctctgcac tcgccggaat aaggaagtcg ccggcggctc cgcttttacc
    35161 cggccatgcg gggcatggcc ttgtgggttt tcagctctgt ggcctcagcg tcgtgtgcgg
    35221 gctgtgccgt gcctccatct tagccgggct ggcagggatg caagggtacg cttcgccgct
    35281 gcggtcaccc ggtccctcct tcccgtctgc cgtgattttc cggtcgttca cccgctcaga
    35341 tttcgcggtc tcaccctcca actcccgcca gccgtgtgcg tcaggctgcg gcttcccttg
    35401 cgccctatgc atccccgcct tatcgcccgg cttttatgga ggcacggcac cgcccggtgc
    35461 cgcagaacat gtgactatgg aggattcggg aatgtctgtt gttgcacctg ctttatacgt
    35521 tggaacctgg cacaaataca actgtggaag catcgccgga cgctggtttg acctgaccac
    35581 gtttgatgat gagcgcgact ttttcgccgc ctgccgtgct cttcaccagg atgaagccga
    35641 tcctgaactg atgtttcagg attatgaggg attcccgggg aatatggcct ctgaatgcca
    35701 tatcaactgg gcctgggttg aaggcttccg ccaggcacgg gatgaaggct gcgaagaggc
    35761 ttatcgtctc tgggtggagg ataccggtga gacggatttt gacaccttcc gcgatgcctg
    35821 gtggggcgag gctgacagtg aggaggcttt tgcggttgag ttcgcctgtg ataccggcct
    35881 gctggctgac gtgccggaga cggtggcgct ctattttgac tatgaggcgt atgcgcggga
    35941 tttattcctg gactccttca cctttattga cggtcatgtg ttccgtcggt gatttactcc
    36001 cccgctccgg cgggggtatg gcctgccagg ccgccggaaa accctggcag gcttacgcct
    36061 gcctgaaatg caggagggcc gcgcctgcgg cgctgccctt cctgcccgtc atggtgtgag
    36121 gcatttttct gttatgtcgt ttctgttgtt ctgatttccg gaggcgggga caccttcggc
    36181 tcccggctgc ctggcggcat ccggtcgtca ggcccgactc cgacaggcag ctgctggcgc
    36241 acccgccagt ctccgccgaa cccgctggcg cggtttcggg ctgccgctac ggtccgatgt
    36301 ctgaaaaacc aggcttgcag cgaccgttcc ggtcgcgccg cccctgtcgc gccggaaacg
    36361 gcgctccctg cacacctgac ggtgtgtaag gccactgcgc ctgatggcgc agtcaaccct
    36421 tccgccgcca cccctgcggc ctgtttcccg ctgtggactt tccgggggct ggagtaacca
    36481 catcccgttt cagcagtcca ggggaagata taccgtccac aaaattttta gccataaacc
    36541 cggcaaaaaa ttttgcgtcc gcccccggac agctgccccg tgacgtggtg acggacgccc
    36601 catcggaaag gtccactgcg gggagaaacc ccgaagaagt ggacggcaga aggggctttc
    36661 gcccctccgg ggagcaaccg gtcggcccgg cgacctgatt cagcagaagg agacgttttc
    36721 atgactgtca gcagcacgat ttccgttttt tgccgcgacg gggtgttccg taccgtttac
    36781 tgccacctgc acggtgagcc gacctggaac ggtcgcatcc tgcataccca ctatgccacc
    36841 ggtcagcagg ccgaagccct ggttgaacac ggtgatatcc gttgcctcgg tccccgttgc
    36901 gacaaacccg ccgggcatac gcttcagaac ccggtggacg gtgtgacggc ttattacgga
    36961 cgtgacagtg gtttccggat ggacagtgag gcgcgtgagt accgttcttt cagggaggct
    37021 attgccactg aaagcactga agaggtgcgc ttccattatg tgttcatcga cggctactgg
    37081 aaggtgatgt accgcacgcc ggaaggctgg aagatgaaag cgctcgcgct ggcactgcgt
    37141 cgctgtccga aatgaaaaaa ggcagggcga taaaccctgc ctctctccgc cggcgcttcc
    37201 ccgccaggaa gtccggcatc tcaatcacta tctatggaga ttatgccatg agaccatcaa
    37261 ttatcttcgc aaccgccgag tatgtaaagc gtctgcgtga agagtgcctg cgggagaata
    37321 aacccctgca ccgccatacc cgcttcagac gtcaggagct ggcacaggat gagattaacc
    37381 cggacgtcct ggcgatgagc ggccatatcg ccagacgctg cagtgagcag aagcgggtgc
    37441 gtatccccgc tatgaaagtc agcgaatggg gacacctgct ccgcgcgctt gaaattgagc
    37501 gggtctgcca ctgattaacc cttgccgtct gcctgtgcag gcggcaggat acgttcagac
    37561 atctcaccag gaggaaccgt gagtaagaag aaaaacacca caacgcccac gccgcatgat
    37621 gccgcgttcc ggtcgttcct ggcgaatccc gacgtcgcca gagattttct ggaactgcat
    37681 cttccggcgg agtaccggca gttgtgcgac ctgtccacgc tgaagctgga acccgccacc
    37741 tttgttgagc cggacctgca tcagtacgcc agcgatatcc tctggagcgt gaaaaccacc
    37801 gggggtgaag atggctatgt ttatacgctc atcgagcacc agagcaccga aaatctgtac
    37861 atgcctttcc gcatgttacg ttacagtgtg gcggcgatgc agagacatct ggagcagcac
    37921 aaaacgttgc cactggtgat tcctgtactg ttctatcatg gtgagcgcag cccgtacccg
    37981 tacagcatga actggctgga ctgttttgag aatccggcac ttgcggctaa aatatacaca
    38041 aagccgtttc cgctggttga tatcactgtc gttgatgaca atgaaatcat gaaccatcgc
    38101 cggatggccg cactgacgct gctgatgaag catatccgcc atcgtgacat gatggagctg
    38161 ctggacaaac tcccgcaggt catggtggaa atttcagatg agcaggtgcg tgttctgatt
    38221 cattacatcg ttaacgcagg ggactctgta tcaccggaat ttatgcgggc gctggctgag
    38281 cgtctgccgc agcatgagga taaactgatg actatcgctg aacgtcttga gcaaaaaggt
    38341 cgccaggagg gcaggatgga aggtgcactt gaaaaagccc tggctattgc gtgccagctt
    38401 cagaaaatgg ggatgacgcc ggagcagatt aggcaggcta ccgggctttc cgatgatgaa
    38461 ctgaagaaaa tcactcactg atgagagtat cacagcccgg caaaatgccg ggctttttat
    38521 ccagcaggag cagagcaaac agcatgacgc ttgcagaacg tcttatacag aaaggtttcg
    38581 acgagggttt taaagaaggt tttaaacaag gttttaaaca aggggctctt gaagtggcgc
    38641 gggaaatagc ctgccggctt cgggatatag gctggccgcc ggaacggatt caggaggtca
    38701 cgggactttc cggtgaagaa ctgaaaaaac tgtttcctga tgagcagtag cctggcattc
    38761 agccaggctg tgaattacgc tcacacatcc tctgccgttt cccccagttc attttccagt
    38821 cgcctgatga tatctgcctt gtgcatcccc tgcatgtgag gcagcaggtc acggactgaa
    38881 agtgcctggg tggtcctcca gacggtgaca tccacctgct ccgcattttc ccaggcgtgc
    38941 catgttctct gtgtcacgcc aaattctctg gccgcttttt cgcgtgacca gagcatgctt
    39001 tttcgccaga ttcgcagttc ccagccggtc ataaaatacc tttgaaaaag tgaaaaaact
    39061 tcactttatt ttatcaccat aatcaccaaa tcagcgcgaa aaataagtgt gaaaaagtga
    39121 agaaatttca ctttttttga ttgtggatgt cgtgctgttt tctctgtttt cacctgtgcg
    39181 ggagtggtgt accagtgacc accgcatgcc gttgttgtgc cgtcggtttt ctccggtcgc
    39241 gccggatgcg gcgctccctg cacacctgac ggtgtgtaag gccactgcgc ctgatggcgc
    39301 tgctatctct gcctgtctgc cgccgcactg cggcctgttc tccgcagacg gactttccgg
    39361 gggtacatac acgaccacat cccgtttcag tcgtccaggg gagagatgta ccagccacaa
    39421 aagtttttgc cgcaggcgtc aaaaactttt gcgtctgccc ccggacaact gccccgtgac
    39481 gtggtgagcg tcccccgtcg gaaaggtcca ctgcggggaa ataccctaag acccggacgg
    39541 cagaaggagc ttcgctcctc cgggaaatga cctggctgac ccggcagtcg tgacgatgag
    39601 gagacaatgt gatgaatcag actttaccca ctgctgacct gaatactgcc ggcacgacag
    39661 atgttattcc gtctgtggct atcgaccgca tcatcgcgca gcgtaacgaa ggtattgcac
    39721 tgttcatgca ggcgatggaa tgcctggcga cagcgcgcag gattctgctc gatgcgtcag
    39781 gtgatatttt tctttacggg tttgaagact gcgtgactga ttccgttcgt cgcatggata
    39841 agccggaaga agcgaaaagg aatatcaccc gtcttgccga ccggaaaatc tgggaccgcc
    39901 tgatgacaga tacgggcatg tacaccttca tgagttcatg ccagcgtgat gagtggaaca
    39961 gccagctgat gagcgacacc tgtcctgaaa tcaccctgga caatgtactg gcaacattcc
    40021 gccatctgaa tgccagtaag atgcagacat ttgaacaggg actgattgat gtctaccgga
    40081 aattgtcatg ggattacaga accaataatc cctgccgtct gggtaagaaa atcattattg
    40141 aaaacctgct gtaccgctgg agtaacgggc gtgtgacgct ggactgcagc ggacgggagg
    40201 cactggatga cctggtacgt ccgttttatc tgctggaggg gcgcaacgtt cctgacttca
    40261 ggaacagcat cggggcgcag tatggtgaat ttctcgggaa cggcgacaat gtcggtaagc
    40321 tgttagaagg ggaatatttt acggtgcgtg gctaccagaa agggaccgta cacattgtct
    40381 ttaagcgttc tgaccttgtt gaaaaactga atgatattat tgcacggcat tatccaggtg
    40441 tattgccgtc acgagtctga ttaaacagaa aagccctggt atttatgccg gggctttttt
    40501 ttctgtaatc atcataatct cagaacacaa tgtgacattg tcacatcaat tgttgttcat
    40561 atcgatgcca tctttcctgc ctgaatctgt ctttttaggt gctgttttag aggaagaacg
    40621 gcgggatggt tttttcgatt ctgcttcccg aataatgtca cgaagtgcct tctctcctac
    40681 ttcataccct gcatttttga gcgtgtcacg aacatctgcc agtgtataac cctttgtcct
    40741 taccaggaca ataatgtcat cacgaatggc atcaagaaaa tcacgcaatg ttttccgttg
    40801 agcggtgaga tctggcaatt ccgataaagc agcttttgcc agctggatgt catcatctga
    40861 gtaaaatttt ttagaagcca ttggcacttt ctctccgttt catgttgaag cctgatttta
    40921 tcatcaaaaa ctgactcctg gcagggctgc gccctgcctc tgccgtctgc ataagactat
    40981 gatgcacaaa aataacaggc tataatggcc tgaaaaacgg gacaggatgt gtaattgtaa
    41041 taccgtcaca cgcgacgcta ttacaattac catctggtca gggcttcgcc ccgacacccc
    41101 gtaaggagcc tgaagtgagt gatagtgcgg taagaaagaa aagtgaggta cggcaaaaga
    41161 cagttgtcag gacactcaga ttctctcctg ttgaggatga aaccatcagg aaaaaagctg
    41221 aagattccgg acttactgta tctgcttaca tccgaaatgc agcactgaat aagcgaatta
    41281 actcccgtac agatgatgct tttctgaagg agttaatgag actgggaagg atgcagaagc
    41341 atctgtttgt tcagggaaaa agaaccggtg acaaggaata cgcagaagta cttgttgcca
    41401 taaccgaact tacaaataca ttgcgtaaac agttaatgga aggttgagga ttctccggtg
    41461 aatgcagtca ttccgaaaaa gagaagggac ggcaagtctt cgttcgaaga cctggtgtcc
    41521 tacgtctcgg tccgggatga catgaccgat gaggaactga atttatcttc ttcttcgcag
    41581 gctgaacaac cgcaccggag ccgtttcagt cgtcttgttg attatgcgac acgccttcga
    41641 aatgagtcat tcgtggcgct ggttgatgtc atgaaggacg gctgcgaatg ggttgacttt
    41701 tacggtgtga cctgttttca caactgtaca tcgcttgaaa ctgctgctgc agatatggag
    41761 tacattgcac agcaggcaca ctatgcgaaa gataatactg atcctgtttt tcactacatt
    41821 ctttcctggc aggcccatga aagcccgcgt ccggaacaga tttatgacag tgtacgccat
    41881 accctgaaat ccctcgggct gggtgaacat cagtatgttt ctgccgtaca taccgatact
    41941 gataacctgc atgttcacgt tgcggtcaac cgggtacatc cggttacagg ttatctcaat
    42001 tgcctttcat ggagtcagga aaaactcagc cgtgcctgcc gtgagcttga actgaagcat
    42061 ggttttgctc cggataacgg ttgctgggtc catgcgccgg gaaatcgtat cgttcgcaaa
    42121 actgccgttg aacgtgatcg ccagaacgct tggacacgcg gaaaaaaaca aacttttcgc
    42181 gagtatgttg cgcagacagc ggtcgctggt ttacgcagtg aacctgtaca tgactggtta
    42241 tccctgcatc gtcgtcttgc ggaagatggt ctgtacctgt ctcagatgga cggaaaattt
    42301 ctggtgatgg atggctggga tcgcaacagg gaaggtgtac agcttgactc gtttggtccc
    42361 tcctggtgtg cagaaaaact catgaaaaaa atgggtgact acacgccagt accaaaagat
    42421 attttcagcc aggtggacgc acccggacgc tataacccgg acttcattgc agctgatgtt
    42481 cgcccggaaa aaatcgctga aacagaaagt ctgcagcagt atgcctgtcg tcatcttgga
    42541 gaacgtctgc cggaaatggc acgggaagga cggctggaaa actgccaggc tattcactgc
    42601 acactggctg aagcgggatt atggatgcgt gttcagcatg gtcacctggt tatctgtgat
    42661 ggttatgatc ataaccagac tcccgttcgt gctgacagcg tatggtcact gctgacgctc
    42721 gacaatgtga atcaactcaa tggtggctgg cagcctgtac caacagatat tttccgccag
    42781 gttacgccaa cagaacgctt ccgtggtcgt cgtatggaga gctgtcctgc gaccgataaa
    42841 gaatggcacc gtatgcgtac aggtacgggg ccgcaggggg ctatcaaacg cgaactgttt
    42901 tctgacaaag aaagtctgtg gggatacagc atcagccatt gtagccctca gattgaagaa
    42961 atgatcacac agggtgagtt tacctggcag cgttgtcatg agttgtttgc acaacaggga
    43021 ctgatgctac agaaacaaca tcacgggctt gttgtcgttg atgcctttaa tcatgagcaa
    43081 acgccggtga aggccagcag cattcatcct gatctgacac tggggcgtgc agagccacag
    43141 gccggacctt ttgtgagtgc cccagcagat ttgtttgaca gggtgcaacc tgaaagtcgc
    43201 tataacccgg agctggctgt cagtgacagg tacggggtca gcagtaaacg tgacccaatg
    43261 ctgcgccggc agcgacgtga agccagagct gaggcccgtg ctgacctgcg tgcccgctat
    43321 ctggcatggc gtgaacaatg gcgtaaacca gatctgcgtt atggggaacg ttgtcgggaa
    43381 attcatcagg catgccgtct gcggaagtca cacattcgtg cgcagtacga tgatccggca
    43441 ttacgtaagc tgcattatca cattgcagaa gttcagcgaa tgcaggcatt gatcaggctg
    43501 aaagaagaca tcagggatga gcggcagaaa cttattgctg acgggaagtg gtatccaccc
    43561 tcttaccgtc agtgggttga aattcaggct gctcagggag acagggctgc tgtatcgcag
    43621 ctgagaggct gggattatcg cgatcgccgt aaagataagt cacgcacaac gacaacagac
    43681 cgctgtgtcg tgctttgtga accgggcgga acgccggtat acggtaatac tggtgatctc
    43741 gaggcccgtt tgcagaagaa cgggagtgtt cgtttccgtg accgtcggac gggagagttt
    43801 gtctgtacgg attatggtga caggattgtt ttccgtaatc accatgaccg caatgcgctg
    43861 gcagataaac tggatttgat tgcaccggta ttattcgggc gtgatccacg catgggtttt
    43921 gaaccagaag gaaacgacaa acaatttaac caggtctttg ctgaaatggt tgcctggtat
    43981 aatgtgacag gccgtaccgg tcacgaagac tacaggatta cgcgaccgga cgtggatcat
    44041 caccgtgaag gaagtgaacg ctactaccgc gattatatcg cggcgaacag caatgatgat
    44101 gcgtcactgc ctccgccgga gcaggataaa ctctgggaac cgccttctcc gatgagatga
    44161 cagatgaagc tttattagct tcgttataga atgaaaaaat caccaatatt aaatatgggt
    44221 gtggtggtaa aaataaatat gttaggcaat gttaagtaac tctattgacg gaatcatgtt
    44281 ttatgagcac taatcttgct tttcgttatg caagtgttga aacttttttt aaaatgattg
    44341 aaaaccaaga attatggttt accgatcttc ggaatatgaa tgatccggaa gaatactcgg
    44401 ctggctatga aatagtcaag agaataataa atgaggaatt tccagattgc attagactat
    44461 tagaagaagt atcacctaat aagatagacg aaaattttaa gatattaatt tgttgtttct
    44521 gttctgatgg agatcgttcc aacatgtgga gcgagtatgg tgataatgg
//
LOCUS       DACBVI010000021        42633 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.021, whole genome shotgun sequence.
ACCESSION   DACBVI010000021 DACBVI010000000
VERSION     DACBVI010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.021"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            253..339
                     /gene="leuL"
                     /locus_tag="IU325_05835"
     CDS             253..339
                     /gene="leuL"
                     /locus_tag="IU325_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HAP5808563.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            432..2003
                     /gene="leuA"
                     /locus_tag="IU325_05840"
     CDS             432..2003
                     /gene="leuA"
                     /locus_tag="IU325_05840"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HAP5808564.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2003..3094
                     /gene="leuB"
                     /locus_tag="IU325_05845"
     CDS             2003..3094
                     /gene="leuB"
                     /locus_tag="IU325_05845"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HAP5808565.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTNEMGDIIARYVVEGV"
     gene            3097..4497
                     /gene="leuC"
                     /locus_tag="IU325_05850"
     CDS             3097..4497
                     /gene="leuC"
                     /locus_tag="IU325_05850"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HAP5808566.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDNAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4508..5113
                     /gene="leuD"
                     /locus_tag="IU325_05855"
     CDS             4508..5113
                     /gene="leuD"
                     /locus_tag="IU325_05855"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HAP5808567.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEEKQPAFMR"
     gene            complement(5221..5352)
                     /gene="sgrT"
                     /locus_tag="IU325_05860"
     CDS             complement(5221..5352)
                     /gene="sgrT"
                     /locus_tag="IU325_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HAP5808568.1"
                     /translation="MRQFYQYYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            5441..7099
                     /gene="sgrR"
                     /locus_tag="IU325_05865"
     CDS             5441..7099
                     /gene="sgrR"
                     /locus_tag="IU325_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SgrR"
                     /protein_id="HAP5808569.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     EIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETISNFASHPIGTGPYGVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDNRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHARP
                     THCEKPAGLESLTLTFYQDHIEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDPE"
     gene            complement(7596..8432)
                     /locus_tag="IU325_05870"
     CDS             complement(7596..8432)
                     /locus_tag="IU325_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP5808570.1"
                     /translation="MKVRNPEQISIPASNTTKDPGLTNSQIIRMVNLVKKTENMNIFE
                     ELWETLRNLFRSDKHSQTAARQILKDAFYFQNCDDYSKYFTGAVDEKARDRFTHRLKK
                     FNELKEHAKDPEMMQARGSISPDNMLCVSFYIGNIEIYTQKLQLGISPSTGGIDLSNA
                     YLSGISLNGACLRKADLSNAEMNKISLCSSNLLGADLHGAKMNNAKVISSDLSDTNLS
                     DTDMSDTDLDDTLLRNAKMDNTILNNAYMEDTNVRGINLSKADLSGQDSAKLRSRGAI
                     ID"
     regulatory      8673..8772
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8803..9786
                     /gene="thiB"
                     /locus_tag="IU325_05875"
     CDS             8803..9786
                     /gene="thiB"
                     /locus_tag="IU325_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HAP5808571.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9762..11372
                     /gene="thiP"
                     /locus_tag="IU325_05880"
     CDS             9762..11372
                     /gene="thiP"
                     /locus_tag="IU325_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HAP5808572.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWS
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPTRAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVLVDGINRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11356..12054
                     /gene="thiQ"
                     /locus_tag="IU325_05885"
     CDS             11356..12054
                     /gene="thiQ"
                     /locus_tag="IU325_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HAP5808573.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTSTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGMTDELL
                     SGKARASALLGITG"
     gene            complement(12168..12932)
                     /locus_tag="IU325_05890"
     CDS             complement(12168..12932)
                     /locus_tag="IU325_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAP5808574.1"
                     /translation="MQALLEHFITQSTVYSLIAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPMYIDILRKVVGG"
     gene            complement(13018..13896)
                     /gene="araC"
                     /locus_tag="IU325_05895"
     CDS             complement(13018..13896)
                     /gene="araC"
                     /locus_tag="IU325_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HAP5808575.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            14235..15935
                     /gene="araB"
                     /locus_tag="IU325_05900"
     CDS             14235..15935
                     /gene="araB"
                     /locus_tag="IU325_05900"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HAP5808576.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHTPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            15946..17448
                     /gene="araA"
                     /locus_tag="IU325_05905"
     CDS             15946..17448
                     /gene="araA"
                     /locus_tag="IU325_05905"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP5808577.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQVHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAAEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17617..18312
                     /gene="araD"
                     /locus_tag="IU325_05910"
     CDS             17617..18312
                     /gene="araD"
                     /locus_tag="IU325_05910"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP5808578.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYNVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18387..20738
                     /gene="polB"
                     /locus_tag="IU325_05915"
     CDS             18387..20738
                     /gene="polB"
                     /locus_tag="IU325_05915"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HAP5808579.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     IYEADVRPPERYLMERFITSPVWVEGDIRNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFKLEYVASRPQLLEKLNAWFATHDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20903..23809
                     /gene="rapA"
                     /locus_tag="IU325_05920"
     CDS             20903..23809
                     /gene="rapA"
                     /locus_tag="IU325_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HAP5808580.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23821..24480
                     /gene="rluA"
                     /locus_tag="IU325_05925"
     CDS             23821..24480
                     /gene="rluA"
                     /locus_tag="IU325_05925"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HAP5808581.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            24693..24871
                     /locus_tag="IU325_05930"
                     /pseudo
     CDS             24693..24871
                     /locus_tag="IU325_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(24888..25649)
                     /locus_tag="IU325_05935"
     CDS             complement(24888..25649)
                     /locus_tag="IU325_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP pyrophosphate phosphatase"
                     /protein_id="HAP5808582.1"
                     /translation="MKVSVPGMPATLLNMSNNDIYKMVSGDKMDVKMNIFQRLWETLR
                     HLFWSDKQTEAYKLLFNFVNKKAGNINASKYFTGAVNENEKEKFIHSLELFNELKTCA
                     KNPDEMVAKGKMRWVAEIYGDIELSVTFFIENKEICTQTLQLHKGPGNLGVDLGEAYL
                     PGVDMRDCYFGKKTIIDSNENLYMKPGWNANLDGATLDGAILDGATVDGATRLYAEVI
                     IINKITPKKIVTEGVATKQSTAEKLLPTQLLNDPE"
     gene            complement(25944..26759)
                     /gene="djlA"
                     /locus_tag="IU325_05940"
     CDS             complement(25944..26759)
                     /gene="djlA"
                     /locus_tag="IU325_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HAP5808583.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            27014..29368
                     /gene="lptD"
                     /locus_tag="IU325_05945"
     CDS             27014..29368
                     /gene="lptD"
                     /locus_tag="IU325_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HAP5808584.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            29421..30707
                     /gene="surA"
                     /locus_tag="IU325_05950"
     CDS             29421..30707
                     /gene="surA"
                     /locus_tag="IU325_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HAP5808585.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            30707..31696
                     /gene="pdxA"
                     /locus_tag="IU325_05955"
     CDS             30707..31696
                     /gene="pdxA"
                     /locus_tag="IU325_05955"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HAP5808586.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     NRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            31693..32514
                     /gene="rsmA"
                     /locus_tag="IU325_05960"
     CDS             31693..32514
                     /gene="rsmA"
                     /locus_tag="IU325_05960"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP5808587.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDKLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            32517..32894
                     /gene="apaG"
                     /locus_tag="IU325_05965"
     CDS             32517..32894
                     /gene="apaG"
                     /locus_tag="IU325_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HAP5808588.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            32901..33749
                     /gene="apaH"
                     /locus_tag="IU325_05970"
     CDS             32901..33749
                     /gene="apaH"
                     /locus_tag="IU325_05970"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase
                     (symmetrical)"
                     /protein_id="HAP5808589.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDMGE
                     GEAAAS"
     gene            complement(33746..34047)
                     /locus_tag="IU325_05975"
                     /pseudo
     CDS             complement(33746..34047)
                     /locus_tag="IU325_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein CcdB"
     gene            complement(34050..34283)
                     /locus_tag="IU325_05980"
     CDS             complement(34050..34283)
                     /locus_tag="IU325_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP5808590.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(34369..34848)
                     /gene="folA"
                     /locus_tag="IU325_05985"
     CDS             complement(34369..34848)
                     /gene="folA"
                     /locus_tag="IU325_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HAP5808591.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(35040..36902)
                     /gene="kefC"
                     /locus_tag="IU325_05990"
     CDS             complement(35040..36902)
                     /gene="kefC"
                     /locus_tag="IU325_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HAP5808592.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFAFSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(36895..37425)
                     /gene="kefF"
                     /locus_tag="IU325_05995"
     CDS             complement(36895..37425)
                     /gene="kefF"
                     /locus_tag="IU325_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HAP5808593.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(37533..38864)
                     /locus_tag="IU325_06000"
     CDS             complement(37533..38864)
                     /locus_tag="IU325_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5808594.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(38923..39210)
                     /gene="fixX"
                     /locus_tag="IU325_06005"
     CDS             complement(38923..39210)
                     /gene="fixX"
                     /locus_tag="IU325_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HAP5808595.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIIVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(39207..40493)
                     /locus_tag="IU325_06010"
     CDS             complement(39207..40493)
                     /locus_tag="IU325_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP5808596.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDESSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(40544..41485)
                     /locus_tag="IU325_06015"
     CDS             complement(40544..41485)
                     /locus_tag="IU325_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAP5808597.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDERMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVCVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(41500..42270)
                     /gene="fixA"
                     /locus_tag="IU325_06020"
     CDS             complement(41500..42270)
                     /gene="fixA"
                     /locus_tag="IU325_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative electron transfer flavoprotein FixA"
                     /protein_id="HAP5808598.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEGQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAT
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVEA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIVAFAENLRKVI"
BASE COUNT        10260 a        11546 c        11059 g         9768 t
ORIGIN      
        1 aacgcgtgca ttttatcccc ctttccctcg tcgattagat gcaaaaattt attctgaaat
       61 atgtcaaccg atgaaaagcg gcggtggtta agcagaaatt aatatcgctt gttttaacca
      121 ccgcagcaca attagctaat ttgacggatg cagaactcat gctggcggga cgtttttatt
      181 acgtcagggt tgacatccgt ttttgtatcc agtaactcta aaagcatatc gcattcatct
      241 ggagctgatt taatgactca catcgttcgc tttatcggtc tactactact aaacgcatct
      301 tctttgcgcg gtagacgagt gagcggcatc cagcattaag ccagcacgca gtcaaacaaa
      361 aaacccgcgc cattgcgcgg gtttttttat gcccgaagcg aggcgctcaa aaagagacaa
      421 ggacccaaac catgagccag caagtcatta ttttcgatac cacattgcgc gacggtgaac
      481 aggcgttaca ggcaagcttg agtgtgaaag aaaaactgca aattgcgctg gcccttgagc
      541 gtatgggtgt tgacgtgatg gaagtcgggt tccccgtctc ttcgccgggt gattttgaat
      601 cagtgcaaac catcgctcgc caggttaaaa acagccgcgt atgcgcgtta gctcgctgcg
      661 tggaaaagga tatcgacgtg gcggccgaat ctctgaaagt cgccgaagcc ttccggattc
      721 atacctttat tgccacttcg ccaatgcaca ttgccaccaa gctgcgtagc acgctggacg
      781 aggtgatcga acgcgctatc tatatggtga aacgcgcccg taattacacc gatgatgttg
      841 aattttcttg cgaagatgcc ggacgcaccc caattgccga tctggcgcga gtggtcgaag
      901 cggcgattaa tgccggtgcc accaccatca acatcccgga caccgtgggc tacaccatgc
      961 catttgagtt cgccggaatc atcagcgggc tgtatgaacg cgtacccaac atcgacaaag
     1021 ccattatttc cgtacatacc cacgacgatt tgggcctggc ggtcggaaac tcactggcgg
     1081 cggtacatgc cggtgcacgc caggtggaag gtgcaatgaa cgggatcggc gagcgtgccg
     1141 gtaactgttc cctggaagaa gtgatcatgg cgatcaaagt tcgtaaggat attctcaacg
     1201 ttcataccgc cattaatcac caggaaatat ggcgcaccag ccaattagtt agccagattt
     1261 gtaatatgcc gatcccggca aacaaagcca ttgttggcag cggcgcattc gcacactcct
     1321 ccggtattca tcaggatggt gtgctgaaaa accgcgaaaa ctacgaaatc atgacaccag
     1381 aatctattgg tttgaaccaa atccagctga atctgacctc tcgttcggga cgtgcggcag
     1441 taaaacaccg catggatgag atggggtata aagaaagtga atataattta gacaatttgt
     1501 acgatgcctt cctgaagctg gcggacaaaa aaggtcaggt gtttgattac gatctggagg
     1561 cactggcctt catcggtaag cagcaagaag agccggagca tttccgtctg gattacttca
     1621 gcgtgcagtc tggctctaac gatattgcca ctgccgccgt caaactggcc tgcggcgaag
     1681 aagtcaaagc agaagccgcc aacggtaacg gtccggtcga tgccgtctat caggcgataa
     1741 accgcatcac tgactataac gtcgaactgg tgaagtacag cctgaccgcc aaaggccacg
     1801 gtaaagatgc gctgggtcag gtggatattg tcgccaacta caatggtcgc cgcttccacg
     1861 gcgtcggcct ggccaccgat attgtcgagt cctccgccaa agccatggtg cacgtactga
     1921 acaatatctg gcgcgccgca gaagtcgaaa aagagttgca acgcaaagct caacacaacg
     1981 aaaacaacaa ggaaaccgtg tgatgtcgaa gaattaccat attgccgttt tgcccggaga
     2041 tgggatcggg ccggaagtga tgacccaggc gctgaaagtg ctggatgccg tgcgcaaccg
     2101 ctttgcgatg cgcatcacca ccagccatta cgatgtaggc ggcgcagcca ttgataacca
     2161 cgggcagccg ctgccgcctg cgacggttga aggttgtgag caagccgatg ccgtgctgtt
     2221 tggctcggta ggtggtccga agtgggaaca tttgccgcca gaccagcaac cagaacgcgg
     2281 cgcactgttg cctttgcgta aacacttcaa attattcagc aatctgcgtc cggcaaaact
     2341 gtatcagggg ctggaagcat tctgtccgct gcgtgctgac attgccgcta acggcttcga
     2401 catcctgtgt gtgcgcgaac tgaccggcgg catctatttc ggtcagccaa aaggccgcga
     2461 aggtagcgga caatatgaaa aagcgtttga taccgaggtg tatcaccgtt ttgagattga
     2521 acgtatcgcc cgcatcgcgt ttgaatctgc tcgtaaacgt cgccataaag tgacgtctat
     2581 cgacaaagcc aacgtgctgc aatcctctat tttatggcgg gagatcgtta acgagatcgc
     2641 cacggaatac ccggatgtcg aactggcgca tatgtacatc gacaacgcca ccatgcagct
     2701 gattaaagat ccatcacagt ttgacgtcct gctgtgctcc aacctgtttg gcgacattct
     2761 gtctgacgag tgcgcaatga tcactggctc aatggggatg ttgccttccg ccagcctgaa
     2821 cgaacaaggt tttggtctgt atgaaccggc aggcggctcc gcaccagata tcgcaggcaa
     2881 aaacatcgcc aacccgattg cacaaatcct gtcgctggca ctgctgctgc gttacagcct
     2941 ggatgccgat gatgcggctt ccgccattga acgcgccatt aaccgcgcat tagaagaagg
     3001 cattcgcacc ggagatttag cccgtggcgc tgccgccgtt agtaccaatg aaatgggcga
     3061 tatcattgcc cgctatgtgg tagaaggggt gtaatcatgg ctaaaacgtt atacgaaaaa
     3121 ttgttcgacg ctcacgtagt gtacgaagcc gaaaacgaaa ccccgctgtt atatatcgac
     3181 cgccacctgg tgcatgaagt gacctcaccg caggcgttcg atggtctgcg cgcccacggt
     3241 cgcccggtac gtcagccggg gaaaaccttc gccactatgg atcacaacgt ctctactcag
     3301 accaaagata ttaatgcctg cggtgaaatg gcgcgcatcc agatgcagga gctgatcaaa
     3361 aactgcaaag aatttggcgt cgagctgtat gacctgaatc acccgtatca ggggatcgtc
     3421 cacgtaatgg ggccggaaca gggcgtcaca ttgccgggga tgaccattgt ctgcggcgac
     3481 tcgcataccg ccacccacgg cgcgtttggc gcactggcct ttggtatcgg cacttccgaa
     3541 gttgaacacg tactggcaac gcaaaccctg aaacagggtc gtgcgaagac catgaaaatt
     3601 gaagtccagg gcaaagccgc gccgggcatt accgcgaaag atatcgtgct ggcaattatc
     3661 ggtaaaactg gcagcgcagg cggcaccggg catgtggtgg agttttgcgg cgaagcaatc
     3721 cgtgatttaa gcatggaagg tcgtatgacc ctgtgcaata tggcaatcga aatgggcgcg
     3781 aaagccggtc tggttgcacc ggacgaaact acctttaact atgtcaaagg ccgtctgcat
     3841 gcgccgaaag gcaaagattt cgacaacgcc gttgcctact ggaaaaccct gcaaaccgac
     3901 gaaggcgcaa ctttcgatac cgttgtgact ttgcaggcag aagaaatttc accgcaggtc
     3961 acctggggca ccaatccagg tcaggtgatt tccgtgaacg acaatattcc cgatccggct
     4021 tcgtttgccg atccggttga acgcgcgtcg gcagaaaaag cgctggccta tatggggctg
     4081 aaaccgggta ttccgctgac agaagtggct atcgacaaag tgtttatcgg ttcctgcact
     4141 aactcgcgta ttgaagattt acgcgcggcg gctgaaatcg ccaaagggcg gaaagtcgcg
     4201 ccaggcgtgc aggcactggt ggttcccggc tctggcccgg taaaagccca ggcggaagcg
     4261 gaaggtctgg acaaaatctt tattgaagcc ggttttgaat ggcgcttgcc aggttgctca
     4321 atgtgtctgg caatgaacaa cgatcgcctg aatccgggtg agcgttgtgc ctccaccagc
     4381 aaccgtaact ttgaaggccg ccaggggcgt ggagggcgca cgcatctggt cagcccggca
     4441 atggctgccg ctgctgctgt gaccggacat ttcgccgaca ttcgcaacat taaataagga
     4501 gcacaccatg gcagagaaat ttatcaaaca cacaggcctg gtggttccgc tggatgccgc
     4561 caatgtcgat accgatgcaa tcatcccgaa acagtttttg cagaaagtga cccgtacagg
     4621 ttttggcgcg catctgttta acgactggcg ttttctggat gaaaaaggcc aacagccaaa
     4681 cccggacttc gtgctgaact tcccgcagta tcagggcgct tccattttgc tggcacgaga
     4741 aaacttcggc tgcggttcct cgcgtgagca cgcgccatgg gcattgaccg attacggttt
     4801 taaagtggtg attgccccga gttttgctga catcttctac ggcaacagct ttaacaacca
     4861 gctgctgccg gtgaaattaa gcgatgccga agtggacgaa ctgtttgcgc tggtgaaagc
     4921 caatccgggg attcattttg atgtagacct ggaagcgcaa gaggtgaaag cgggagagaa
     4981 aacgtatcgc tttaccatcg atgccttccg ccgccactgc atgatgaacg gtctggacag
     5041 catcggcctg acattgcagc acgacgacgc cattgcgtct tatgaagaga agcaaccggc
     5101 atttatgcgt tagtttgtaa accgtccggc actcaatcgc caaaaagaaa aaagccagca
     5161 gataatcact gctggcgtgg cgattaacac caatactcag tcacacatga tgtccgcaaa
     5221 tcagtagtca gaatgactcc cctcccactg catcagttct tcaagcatag ttaatcgttg
     5281 tgggacgctt aaccaacgca accagcacaa cttcgctgtc gcggtaaaat agtactgata
     5341 aaactgacgc atggggcacc tccttgcttc atcgttagga attattgact taatataggg
     5401 aaaataaaat tgctgtcttt tgcgcaggag ttcccctttt atgccatctg ctcgtctgca
     5461 acaacagttc atccgcctgt ggcaatgctg cgagggtaaa tcgcaggaca caacgctcaa
     5521 cgaactggca gcgttattga gctgctcgcg tcgtcatatg cgcaccctgc tcaacaccat
     5581 gcaggatcgc ggctggctga cgtgggaagc ggaagttggg cgcggtaaac gctcgcgtct
     5641 gacattcctc tacaccgggc tggcgcttca gcaacagcgg gcggaagacc tgctggagca
     5701 ggatcgtatc gatcaactag tgcagttggt tggcgacaaa gcgactgtgc ggcaaatgct
     5761 ggtttctcat ctgggacgca gcttccgcca ggggcggcac atcctgcgcg tgctctacta
     5821 tcgtccgttg cgtaatctgc tgcccggcag tgcattgcgc cgttccgaaa cccatatcgc
     5881 ccggcaaatc ttcagttcgc taacgcgcat aaatgaggaa aatggggaac tggaagcgga
     5941 catcgcccac cactggcagc aaatatcacc gcttcactgg cgtttctttt tgcgtccagg
     6001 agtccatttt catcatggtc gtgaactgga aatggacgat gtgatcgcct ctttaaaacg
     6061 gatcaatacg ctgccgctct attcgcatat tgctgaaatt gtatcgccga cgccctggac
     6121 gctggatatc cacctcacgc agccggaccg ctggttgccg ttactgctgg gacaagttcc
     6181 ggcgatgatc ctgccgcgcg aatgggaaac catcagtaac tttgccagcc atcccatcgg
     6241 caccggtccg tatggggtga tccgcaacag caccaatcaa ctgaaaattc aggcattcga
     6301 tgacttcttc ggttaccggg cattaatcga cgaagttaac gtctgggttc taccggaaat
     6361 tgccgacgag ccagccggag ggctgatgct aaaaggtcca cagggcgagg aaaaagagat
     6421 tgaaagccgc ctggaggaag gttgctacta tttactgttc gacaaccgca cccatcgcgg
     6481 ggcgaatcag caagtcaggg actgggtaag ctatgtgctt tctccaacta atctggtcta
     6541 tttcgctgag gaacagtacc agcaactgtg gttcccggct tatggactgc tcccacgttg
     6601 gcaccatgct cgtccgacac attgcgaaaa acccgccggg ctggaaagcc tcaccctgac
     6661 cttttatcag gatcatattg agcatcgagt gattgccggg atcatgcagc agattctggc
     6721 aagtcaccag gtcacgctgg aaatcaaaga gatcagctac gatcagtggc atgaaggaga
     6781 gatcgagagc gatatctggc ttaacagcgc caactttacg ctgccgctgg atttttcgct
     6841 attcgcgcac ctgtgcgagg tgccgctgct ccaacactgt attccgatcg actggcaagc
     6901 cgacgccgcc cgctggcgca atggcgaaat gaacctggcg aactggtgcc agcaactggt
     6961 cgccagcaaa gcaatggtgc cgcttatcca ccactggctg attattcagg gacaacgcag
     7021 tatgcgcggc ctgcgtatga acaccctcgg ctggttcgat tttaaatcag cgtggtttgc
     7081 gccgcctgat ccagagtagc tctcgccttt tggcggtgat gagcgcgttt tttatattca
     7141 gacggaatga ataaaataca ttacttttat attttctctg ttttatgcat cgtctgatta
     7201 gagtcgcaaa taagattatc tcgaatcgtg tgcgattcat tgtctgaaaa atgacgactc
     7261 cgcactcact gaaaacaagg cagagtctgg attcgacttc agactccaac ttaaatttct
     7321 tttgccagaa taaaaataca cgctcataat ttcagtaaca cgctgttgtt tgaggctaat
     7381 cagacgagat tttatcttgc gtgggtgtgg gatgttgatg ttaagtgaca ggcatatgaa
     7441 aattagcata ccgggtagga ttcagtattt ctgcgcaata ttgttttata taaaaaataa
     7501 gtcagtgtgt tttttacctt attttatata tagtggtcag caccaactta aaaaagaaag
     7561 ggcgttcctg ccccttccct atttattatt ctggctcaat caataattgc gccacgactg
     7621 cgcaatttag cgctatcctg gccggaaagg tcagccttgc tcaggttaat cccgcgcacg
     7681 ttagtatctt ccatataagc attgttcagg atggtattgt ccatttttgc attgcgcagg
     7741 agtgtatcgt ccaggtcagt atcggacatg tcagtatcgg acaggttagt atcggacagg
     7801 tcagagctaa taactttggc attgttcatt ttagcaccgt gcaggtcagc accgagtagg
     7861 ttagaactgc acagactgat cttgttcatt tcagcattgc tcaggtcagc cttgcgaagg
     7921 caagcaccat taagggaaat cccggaaagg taagcattac tcagatcaat gcccccggta
     7981 ctggggctta ttcccaattg caacttctga gtatatatct ctatatttcc aatataaaaa
     8041 ctgacacata acatattatc tggcgagatt gagcccctcg cctgcatcat ttccggatct
     8101 tttgcgtgtt ctttgagttc attaaatttt tttagccggt gggtaaacct atcacgcgcc
     8161 ttctcgtcga cagccccggt aaagtatttt gagtagtcat cacaattctg gaaataaaat
     8221 gcatccttca gaatttgcct tgccgcagtc tgggaatgtt tatctgaccg gaagagattg
     8281 cgtaacgtct cccatagctc ttcaaagata ttcatattct cggtcttttt gaccaggtta
     8341 accatcctga taatctgaga attagtaagg cctggatctt tagtggtatt acttgcaggg
     8401 atactaatct gttctgggtt tcttactttc atgctgatag ctccactctg agttaaaaac
     8461 tcagaataag cgtcctgaat aagaatactg tcaaaaatcg ctcattcacg caataagccg
     8521 ttgcattacc gattttgact gtgctaaggg attctttcgg ttcaaagctt cccagctatg
     8581 caaatacgtc cttgatagct gaacgaatat tggctttttt aatacacctt ctctatggat
     8641 tgctggttga taacaaaatc actacactaa cgccgttctc aacggggtgc cacgcagacg
     8701 cgtgcgctga gaaaataccc gtcgaacctg atccggataa cgccggcgaa gggatttgag
     8761 gctccttctc aagtcctttg ccactctttt ttgaggtgca aagtgttaaa aaaatgtctt
     8821 cccctgctgt tgctgtgcac agcgcccgtt ttcgctaaac ccgttctgac tgtttatacc
     8881 tacgattcct tcgccgccga ctgggggcct ggtccaaaga tcaaaaaagc ttttgaagcc
     8941 gactgtaact gcgaactgaa actggtggcg ctggaagatg gcgtttcgct gctcaaccgt
     9001 ctgcggatgg aaggcaaaaa cagtaaagcc gatgtggtgc tggggctgga taacaacctg
     9061 ttagatgccg ccagtaaaac cggactattt gccaaaagcg gtgtggcagc ggatgccgtt
     9121 aacgttcccg gcggctggaa taatgacact ttcgtcccgt ttgattacgg ctatttcgcc
     9181 ttcgtctatg acaagaacaa actgaaaaat ccgccgcaaa gcctgaaaga gttggttgaa
     9241 agcgatcaaa actggcgggt gatttatcag gatccgcgca ccagcacgcc gggactgggt
     9301 ctgctgttgt ggatgcaaaa agtctatggc gatgacgccc cacaagcctg gcagaaactg
     9361 gcgaagaaaa cggtcacggt caccaaaggc tggagcgaag cctacggcct gtttttaaaa
     9421 ggtgaaagcg atctggtact gagttacacc acctctccgg cttatcacat tctcgaagag
     9481 aagaaagata actacgccgc cgcgaacttc agcgaaggtc actatttgca agtggaagtc
     9541 gccgcccgca ccgctgccag caagcagcca gagctggcgc aaaaattcct ccagtttatg
     9601 gtttctccgg cttttcagaa tgcgatccca accggcaact ggatgtatcc ggtggcaaac
     9661 gtcacgctgc ctgccggttt tgaacaattg accaaaccgg caaccacgct ggagttcacg
     9721 ccagccgaag tggcggcaca acgtcaggca tggattagcg aatggcaacg cgccgtcagc
     9781 cgttaattcc cggctggtta attccaggtg taagcgccgc tacgcttgtg gtggcggttg
     9841 cgctggcggc gtttctcgcc ctgtggtgga acgcgccgca gggtgactgg tcggcagtct
     9901 ggcaggacag ctacctgtgg catgtggtgc gcttctcctt ctggcaggcg tttctctcgg
     9961 cgctactctc tgtcgtaccc gcgatattcc tcgcccgcgc gctctatcgc aggcgctttc
    10021 cgggtcggct ggcgctgttg cgtctgtgcg caatgacctt gatcctcccg gtgctggttg
    10081 ctgttttcgg cattcttagc gtctatggtc gccagggctg gctggcatcg ctctgccaat
    10141 cgctcggtct ggagtggacc ttttcgccct acggcctgca aggcattttg ctggcccatg
    10201 tgttttttaa tctgccgatg gcgagccgtt tattacttca ggcactggaa aacattcccg
    10261 gcgaacaacg tcaacttgcc gcccagcttg ggatgcgtgg ctggcacttc tttcgtttcg
    10321 tcgaatggcc gtggttacgg cgacaaatcc cgccagttgc tgcgcttatc tttatgctct
    10381 gtttcgccag cttcgccacc gtgttgtcgc tggggggcgg tccgcaggcg accactatcg
    10441 aactggctat ttatcaggcg ctgagttacg actacgatcc cacccgcgcg gcgatgttgg
    10501 cgctgatcca gatggtgtgt tgccttgggc tggtgctgct gagtcagcga ttgagtaagg
    10561 ccattgcgcc aggcaccacg ctgctgcaag gctggcgcga cccggacgat cgtctgcata
    10621 gccgcatttg cgacacggtg ttaattgtgc tggcgctgct gctattgctg ccaccgttac
    10681 tggcggtgct cgtcgatggg ataaatcgcc agttgccgga agtgctggcg cagccggtgc
    10741 tgtggcaggc gctgtggacc tcgttgcgta ttgcgctggc ggcaggcgta ttgtgcgtag
    10801 tgctgaccat gatgctgcta tggagcagtc gcgaacttcg ggcgcggcag aaaatgctgg
    10861 ccggtcaggc gctggaaatg agcggcatgt tgatcctcgc catgccgggg attgtgctgg
    10921 ctaccggctt ctttttactg ctcaacaaca ctatcggcct gccgcaatct gctgacggca
    10981 ttgtgatttt caccaatgcg ttaatggcga tcccttatgc gctgaaggtg ctggaaaacc
    11041 cgatgcgcga tatcaccgcg cgttacagta tgttgtgtca gtcgctgggc attgaaggct
    11101 ggtcacgctt aaaagtggtg gagctgcgcg ccctgaaacg cccacttgcg caggcactgg
    11161 cctttgcttg tgtgctatcg attggtgatt ttggcgtggt ggcgttgttc ggtaacgatg
    11221 atttccgcac cctgccgttt tatctctacc agcaaattgg ctcctatcgc agccaggacg
    11281 gcgcggtcac cgcgttaatt ctgctgctac tctgttttct gctgtttacc gtgattgaaa
    11341 aactaccggg gcgaaatgtt aaaactgact gatatcacct ggctttacca ccatttgccg
    11401 atgcgtttta gcttaacggt ggaacgaggc gagcaggtgg cgatcctcgg gccaagcggc
    11461 gcgggtaaaa gtaccctgct gaatttgatc gccggttttc tgacgccagc cagcggttcg
    11521 ctgactatcg acggcgttga tcacacctct acgccgccgt cacgccgtcc ggtgtcgatg
    11581 ctgtttcagg agaacaacct gttcagtcac ctgacggtcg cgcagaacat cgggctgggg
    11641 cttaatccag gattgaagct gaacgcggca caacaggaga aaatgcacgc tatcgcccgc
    11701 cagatgggga ttgataattt aatggcgcga ttaccgggcg agctttctgg cggtcagcga
    11761 cagcgcgtgg cgttagcgcg ttgtctggtg cgcgaacagc cgattttatt gctcgatgaa
    11821 ccgttctctg cgctcgatcc ggcgttacgt caagagatgt tgacgctggt gagcacgagc
    11881 tgccagcagc aaaaaatgac gctattgatg gtgtcgcaca gtgtggaaga tgcggcgcgg
    11941 atcgccacac gctcggtggt ggtcgccgac gggcgcatcg cctggcaggg tatgaccgat
    12001 gagttgttga gcggtaaggc gagagcttcg gcactattgg ggattacggg ttagtatgca
    12061 ctgtcggatg cggcgtgaac gccttatccg acctacggac agtgcgtttt gtaggcctga
    12121 taagacgcgg caagcgtcgc atcaggcaac ccgcacaagg ctatccccta acccccaacc
    12181 actttacgca aaatatcgat atacatcggc atcaacgggt ggcgaattaa caccaccagc
    12241 gccaccacgc cgatggcaga aatcaacggc gtcagccaca acaaacgacc gcgcgacaaa
    12301 taatgactta aacgatcggt tgctttaccg ctgcgccata accgccagca cagccagcca
    12361 ccaacccaca aaaataccgc cgtcgccagc agcaaccatt taaactcgcc gctctgcatt
    12421 ccggctggaa tatcgatcgc cgcacccgcc agaatccccg gcaggaagta aaacggtggc
    12481 cacagcaggc agccgataat attcggcgta ataaattttg ccaccggcag atccagcatt
    12541 cccgccacca ttggcaccag cggacgcgtc gggccaacaa aacgaccgac cagaatggtg
    12601 aacatgctgt gttgatgcaa cgcatgttca gttttatcaa gtagtgcttt gtttttcttc
    12661 agaaatgacc agcgatgcaa tggcttttta aaacgccagc ccagccagaa agagatccag
    12721 tcgcccagca ggcaaccgac gatccctgcc agccaggcgt gccagaaact taactcgccg
    12781 ctgccaatca gcgcccccag ccccgccatc agcaccgtac cgggtagaat caaaccgacc
    12841 agcgccagcg actccagaaa ggccaccaac accaccgcta tcaatgaata cacggtggat
    12901 tgggtaataa agtgttccag caatgcttgc ataatgtgcc tgtcaaatgg acgaagcagg
    12961 gattctgcaa accctatgct actccgtcaa gtcgtcaatt gtctgattcg ttaccaatta
    13021 tgacaacttg acggctacat cattcacttt ttcttcacaa ccggcacgga attcactcgg
    13081 gctggccccg gtgcattttt taaatactcg cgagaaatag agttgatcgt caaaaccaac
    13141 attgcgaccg acggtagcga taggcatccg ggtggtgctc aaaagcagct tcgcctgact
    13201 aatgcgttgg tcctcgcgcc agcttaagac gctaattcct aactgctggc ggaacagatg
    13261 cgacagacgc gacggcgaca ggcaaacatg ctgtgcgacg ctggcgatat caaaattgct
    13321 gtctgccagg tgatcgctga tgtactgaca agcctcgcgt acccgattat ccatcggtgg
    13381 atggagcgac tcgttaattg cttccatgcg ccgcagtaac aattgctcaa gcagatttat
    13441 cgccagcagc tccgaatagc gcccttcccc ttgcccggcg ttaatgattt gcccaaacag
    13501 gtcgctgaaa tgcggttggt gcgcttcatc cgggcgaaag aaacccgtat tggcaaatat
    13561 tgacggccag ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa cccactggtg
    13621 ataccattcg cgagcctccg gatgacgacc gtagtgatga atctctcctg gcgggaacag
    13681 caaaatatca cccggtcggc agacaaattc tcgtccctga tttttcacca ccccctggcc
    13741 gcgtatggtg agattgagaa tataaccctt cattcccagt ggtcggtcga taaaaaaatc
    13801 gagataaccg ttggcctcaa tcggcgttaa acccgccacc agatgggcgt taaacgagta
    13861 tcccggcagc aggggatcat tttgcgcttc agccatactt ttcatactcc cgccattcag
    13921 agaagaaacc aattgtccat attgcatcag acattgccgt caccgcgtct tttactggct
    13981 cttctcgcta atccaaccgg taaccccgct tattaaaagc attctgtaac aaagcgggac
    14041 caaagccatg acaaaaacgc gtaacaaaag tgtctataat cacggcagaa aagtccacat
    14101 tgattatttg cacggcgtca cactttgcta tgccatagca tttttatcca taagattagc
    14161 ggatcctacc tgacgctttt tttcgcaact ctctactgtt tctccatacc cgtttttttg
    14221 gatggagtga aacgatggcg attgcaattg gcctcgattt tggcagtgat tctgtgcgag
    14281 ctttggcggt ggactgcgcc accggtgaag agatcgccac cagcgtagag tggtatcccc
    14341 gttggcagaa agggcaattt tgtgatgccc caaataacca gttccgtcat catccgcgtg
    14401 actacattga gtcaatggaa gcggcgctga aaaccgtgct tgcagagctt agcgccgagc
    14461 agcgcgcagc tgtggtcggg attggcgttg acacaaccgg ctcgacgccc gcaccgattg
    14521 acgcggacgg taacgtcctg gcgctgcgcc cggagtttgc cgaaaacccg aacgcgatgt
    14581 tcgtattgtg gaaagaccac accgcggttg aagaagcgga agagattacc cgcttatgtc
    14641 acacgccggg caacgttgac tactcccgct atattggcgg tatttattcc agcgaatggt
    14701 tctgggcaaa aatactgcat gtaactcgcc aggacagcgc cgtggcgcaa tctgccgcat
    14761 cgtggattga gctgtgcgac tgggtgccag ctctgctttc cggtaccacg cgcccgcagg
    14821 atattcgtcg cggacgttgc agcgccgggc ataagtcgct atggcacgaa agctggggcg
    14881 gcctgccgcc agccagtttc tttgacgaac tggacccgat cctcaatcgc catttgcctt
    14941 ccccgctgtt cactgatacc tggactgccg atattccggt gggcacctta tgcccggaat
    15001 gggcgcagcg tctcggcctg cctgaaagcg tggtgatttc cggcggcgcg tttgactgcc
    15061 atatgggcgc agttggcgca ggcgcacagc ctaacgcact ggtaaaagtt atcggtactt
    15121 ccacctgcga cattctgatt gccgacaaac agagcgttgg cgagcgggca gtgaaaggta
    15181 tttgcggcca ggttgatggc agcgtggtgc ctggatttat cggtctggaa gcaggccaat
    15241 cggcgtttgg cgatatctac gcctggttcg gtcgcgtact cggctggccg ctggaacagc
    15301 ttgccgccca gcatccggaa ctgaaagagc aaatcaacgc cagccagaaa caactgcttc
    15361 cagcgctgac cgaagcatgg gccaaaaatc cgtctctgga tcacctgccg gtggtgctcg
    15421 actggtttaa cggccgccgc acaccgaacg ctaaccaacg cctgaaaggg gtgattaccg
    15481 atctgaacct cgctaccgac gctccgctgc tgttcggcgg tttgattgct gccaccgcct
    15541 ttggcgcacg cgcaatcatg gagtgcttta ccgatcaggg gatcgccgtt aataacgtga
    15601 tggcgctggg cggcatcgcg cggaaaaacc aggtcattat gcaggcctgc tgcgacgtgc
    15661 tgaatcgccc gctgcaaatt gttgcctctg accagtgctg tgcgctcggt gcggcgattt
    15721 tcgctgccgt cgccgcgaaa gtgcacgcag acatcccatc agcccagcaa aaaatggcca
    15781 gtgcggtcga gaaaaccctg caaccgcgca gcgaacaggc acaacgcttt gaacagcttt
    15841 atcgccgcta tcagcaatgg gcgatgagcg ccgaacaaca ttatcttcca acttccgccc
    15901 cggcacaggc tgcccaggcc gttccgactc tataaggaca cgacaatgac gatttttgat
    15961 aattatgaag tgtggtttgt aattggcagc cagcatctgt atggcccgga aaccctgcgt
    16021 caggtcaccc aacatgccga gcacgtcgtt aatgcgctga atacggaagc gaaactgccc
    16081 tgcaaactgg tgctgaaacc gctgggcacc acgccggatg aaatcaccgc tatttgccgt
    16141 gacgcgaatt acgacgatcg ttgcgccggc ctggtagtgt ggctgcacac tttctccccg
    16201 gccaaaatgt ggatcaacgg cctgactatg ctcaacaaac cgttgctgca attccacacc
    16261 cagttcaacg cggcgctgcc gtgggatagc atcgatatgg actttatgaa cctgaaccag
    16321 actgcacatg gcggtcgcga gttcggcttc attggcgcgc gtatgcgtca gcaacatgcc
    16381 gtcgttaccg gtcactggca ggataaacaa gtacatgagc gtatcggctc ctggatgcgt
    16441 caggcggtat ctaaacagga tacccgtcat ctgaaagtct gccgttttgg cgataacatg
    16501 cgtgaagtgg cggtcaccga tggcgataaa gttgccgcac agatcaagtt cggtttctcc
    16561 gtcaatacct gggcggttgg cgatctggtg caggtagtga actccatcag cgatggcgat
    16621 gttaacgcgc tggtcgatga gtacgaaagc tgctacacca tgacgcctgc cacacaaatc
    16681 cacggcgaaa aacgccagaa cgtgctggaa gcggcgcgta ttgagctggg gatgaagcgt
    16741 ttcctggaac aaggtggctt ccacgcgttc accaccacct ttgaagattt gcacggtctg
    16801 aaacagcttc ctggtctggc cgtacagcgt ctgatgcagc agggttacgg ctttgcgggc
    16861 gaaggcgact ggaaaactgc cgccctgctt cgcatcatga aggtgatgtc aaccggtctg
    16921 cagggcggca cctcctttat ggaggactac acctatcact tcgagaaagg caatgacctg
    16981 gtgctcggct cccacatgct ggaagtctgc ccgtcgatcg ccgcagaaga gaaaccgatc
    17041 ctcgacgttc agcatctcgg tattggtggt aaggacgatc ctgcccgcct gatcttcaat
    17101 acccaaaccg gcccagccat tgtcgccagc ttgattgatc tcggcgatcg ttaccgtctg
    17161 ctggttaact gcatcgacac agtgaaaaca ccgcactccc tgccgaaact gccggtggcg
    17221 aatgcgctgt ggaaagcgca accggatctg ccaactgctt ccgaagcgtg gatcctcgct
    17281 ggtggcgcgc accataccgt tttcagccat gcgctgaacc tcaacgatat gcgccagttc
    17341 gccgagatgc acgacattga aatcacggtg attgataacg acacccgcct gccagcgttt
    17401 aaagacgcgc tgcgctggaa cgaagtgtat tacgggtttc gtcgctaagt agccgcatca
    17461 ggtgtgtaac gcctgatgcg acgctgacgc gtcttatcag gcctacacgc tgtgattttg
    17521 taggccggat aagcaaagag catccggcat tgttttcccc ctcgcccctt tggggagagg
    17581 gccggggtga ggggaaacga cctctcagga gtcaacatgt tagaagatct caaacgccag
    17641 gtattagaag ccaatttggc gctgccaaaa cataacctgg tcacgctcac atggggcaac
    17701 gtcagcgccg ttgatcgcga gcgcggcgtc tttgtgatca aaccttccgg cgtcgattac
    17761 aacgtcatga ccgctgacga tatggtcgtg gtcagcatcg aaaccggtga agtggttgaa
    17821 ggaacgaaaa agccctcctc cgacacgcca actcaccggc tgctctatca ggcattcccg
    17881 tccattggcg gcattgtgca cacacactcg cgccacgcca ccatctgggc gcaggcgggt
    17941 cagtcgattc cagcaaccgg taccacccac gccgactatt tctacggcac cattccctgc
    18001 acccgcaaaa tgaccgacgc agaaatcaac ggtgaatatg agtgggaaac cggtaacgtc
    18061 atcgtagaaa ccttcgaaaa acagggtatc gacgcagcac aaatgcccgg cgtcctggtc
    18121 cattctcacg gcccgtttgc atggggcaaa aatgccgaag atgcggtgca caacgccatc
    18181 gtgctggaag aagtcgctta tatggggatt ttctgccgcc agcttgctcc gcagttaccg
    18241 gatatgcagc aaacgttgct ggataaacat tatttacgta agcatggtgc gaaggcatat
    18301 tacgggcagt aataactgta taaaaccaca gccaatcaaa cgaaaccagg ctataatcaa
    18361 gcctggtttt ttattggatt tttagcgtgg cgcaggcagg ttttatctta acccgacact
    18421 ggcgggatac cccgcaagga accgaagttt ccttttggtt agcgacggac aacgggccgt
    18481 tgcaggttac gcttgcaccg caagagtccg tggcctttat tcccgccgat caggttcccc
    18541 gcgcccagca tattttgcag ggtgagcaag gctttcgcct gacgccgctg gcgttaaagg
    18601 attttcatcg ccagccggtg tatggccttt actgtcgcgc ccatcgccag ttgatgaatt
    18661 acgaaaagcg cctgcgtgaa ggtggcgtta ccatctacga ggccgatgtg cgcccgccag
    18721 aacgctatct gatggagcgg tttatcacct caccggtgtg ggtcgagggt gatatacgca
    18781 atggcgctat cgttaatgcc cgtctgaaac cgcatcccga ctaccgtccg ccgctcaagt
    18841 gggtttctat tgatattgaa accacccgcc acggtgagct gtactgcatc ggcctggaag
    18901 gctgcgggca gcgcatcgtt tatatgctgg ggccagagaa tggcgacgcc tccgcgctcg
    18961 attttaaact ggaatatgtc gccagtcgcc cgcagctact ggaaaaactc aacgcctggt
    19021 ttgcgactca cgatcccgat gtgatcatcg gctggaacgt ggtgcaattc gatctacgaa
    19081 tgctgcaaaa acatgccgag cgttatcgta ttccgctgcg gctggggcgc gataacagtg
    19141 agctggagtg gcgcgagcac ggctttaaaa acggcgtctt ttttgcccag gccaaagggc
    19201 gactgattat cgacggtatc gaggcgctga aatccgcgtt ctggaatttc tcttcattct
    19261 cgctggaaac cgtcgctcag gagttattag gcgaaggaaa atctatcgat aacccgtggg
    19321 atcggatgga cgaaattgac cgccgtttcg ccgaagataa acctgcgctg gcaacttata
    19381 acctgaaaga ttgcgagctg gtgacgcaga tcttccacaa aactgaaatc atgccgtttt
    19441 tactcgaacg agcgacggtg aacggcctgc cggtagatcg gcacgggggg tcggtggcag
    19501 cgtttggtca tctctatttt ccccgaatgc atcgcgctgg ttatgtcgcg cctaatctcg
    19561 gcgaagtgcc gccgcacgcc agccctggcg gctatgtgat ggattcgcgg cctgggcttt
    19621 atgattcggt gctggtgctg gactacaaaa gcctgtaccc gtcgatcatc cgcacctttc
    19681 tgattgatcc cgtcggactg gtggaaggca tggcgcagcc tgatccagag cacagcaccg
    19741 aaggctttct cgatgcctgg ttctcgcggg agaaacattg cctgccggag attgtgacta
    19801 acatctggca cgggcgcgat gaagccaaac gtcagggtaa taagccgctt tcgcaggcgc
    19861 tgaagatcat tatgaatgcc ttttatggcg tgcttggcac caccgcctgc cgcttcttcg
    19921 atccgcgcct ggcgtcgtcg atcaccatgc gtggtcatca gatcatgcgg caaaccaaag
    19981 cgttgattga agcgcagggc tacgacgtga tctacggcga taccgactca acgtttgtct
    20041 ggttgaaagg cgcacattcg gaagaagaag cggcgaaaat tggtcgtgca ctggtgcagc
    20101 acgttaacgc ctggtgggca gaaacgctgc aaaaacaacg actgaccagc gcactggaac
    20161 tggagtatga aacccatttc tgccgttttc tgatgccaac cattcgcgga gccgataccg
    20221 gcagtaaaaa gcgttatgcc gggctgattc aggagggcga taaacagcgg atggtgttta
    20281 aagggctgga aaccgtgcgc accgactgga cgccgctggc ccagcagttt cagcaggagc
    20341 tgtacctgcg tatcttccgc aacgagccat atcaggagta cgtccgcgaa accatcgaca
    20401 aactgatggc gggtgaactg gatgcgcggc tggtttaccg taaacgcctt cgccgtccgc
    20461 tgagcgaata tcagcgtaat gttccgcctc atgtacgcgc cgctcgcctt gccgatgaag
    20521 aaaaccaaaa gcgcggtcgc cccttgcaat atcagaaccg cggcaccatt aagtacgtat
    20581 ggaccaccaa cggcccggag ccgctggact accaacgttc accactggat tacgaacact
    20641 atctgacccg ccagctacaa cccgtggcgg agggaatact cccttttatt gaggataatt
    20701 ttgctacact tatgaccggg caacttgggc tattttgagc aaaaaaaaga gttcgccaga
    20761 taccattttg atgcgtgacg aatgctttgc catccagtac catagcgccc tttccattcc
    20821 tggacctgaa taacaccact acctcataag cacggtagcg ggtggttatt gcctgcaatt
    20881 aaagatatag agccgaacac atatgccttt tacacttggt caacgctgga tcagcgatac
    20941 agaaagcgaa ttgggacttg gaaccgttgt cgcggtggat gcgcgaactg tcactttact
    21001 tttcccatct actggtgaaa accgtctgta cgcacgcagt gattcccccg tgacccgcgt
    21061 gatgttcaac cctggtgata ccattaccag ccatgacggc tggcagatgc aagtcgaaga
    21121 agtaaaagaa gaaaatggct tgctgaccta tatcggtact cgcctggata ctgaagagtc
    21181 cggcgtagcc ctgcgtgaag ttttccttga tagcaaactg gtgttcagca aaccgcagga
    21241 ccgtctgttt gccgggcaga ttgaccgtat ggaccgcttt gcgctgcgtt atcgcgcgcg
    21301 taagtattcc agcgaacagt tccgtatgcc gtacagcggc ctgcgcggtc agcgtaccag
    21361 cctgatcccg caccagctca acatcgctca tgatgttggc cgccgccacg cgccgcgcgt
    21421 cctgctggct gacgaagtgg gtttagggaa aaccattgaa gccgggatga tcctgcatca
    21481 gcaactgctc tctggcgctg ctgaacgtgt gctgattatc gtcccggaaa ccttacagca
    21541 tcagtggctg gtagaaatgc tgcgccgttt caacctgcgc tttgctctgt ttgatgatga
    21601 gcgttatgcc gaagctcagc acgatgctta caacccgttc gataccgaac agctggtgat
    21661 ttgctcgctg gattttgccc gtcgtagcaa acagcgtctg gaacatctct gtgaagccga
    21721 atgggatctg ctggtggtcg atgaagccca tcacctggtg tggagcgaag atgcgccgag
    21781 ccgcgaatat caggccattg aacaactggc agagcatgtg ccgggcgttc tgctgctgac
    21841 cgcaaccccg gaacagctgg ggatggaaag ccacttcgcc cgtctgcgtc tgctggaccc
    21901 gaaccgtttc cacgattttg cgcaattcgt tgaagagcag aaaaattatc gtccggttgc
    21961 ggacgccgtt gccatgctgc tggcaggtaa caagctgagc aatgacgaac tgaacatgct
    22021 tggcgagatg atcggcgagc aggatatcga gccgctgtta caagcagcaa acagcgacag
    22081 cgaagatgcc cagagcgccc gtcaggagct ggtttcgatg ctgatggatc gccacggcac
    22141 cagccgcgtg ctgttccgta acacgcgtaa cggtgtgaaa ggcttcccga aacgcgagct
    22201 gcacaccatt aagctgccgc tgccgacgca gtatcagacg gctattaaag tctccggcat
    22261 tatgggcgca cgcaaaagtg cggaagatcg cgctcgcgat atgctctacc cggagcgtat
    22321 ttatcaggaa tttgaaggtg ataacgccac ctggtggaac ttcgatccgc gcgttgagtg
    22381 gctgatgggc tacctgacca gccatcgctc tcagaaagtg ctggtgatct gcgctaaagc
    22441 tgccactgcg ctgcaactgg agcaagtact gcgcgaacgt gaaggtatcc gcgctgctgt
    22501 gttccacgaa ggcatgtcga ttattgagcg tgaccgcgct gccgcctggt ttgctgaaga
    22561 agacaccggt gcgcaggtac tgctgtgctc ggaaatcggt tctgaaggac gtaacttcca
    22621 gttcgccagc cacatggtga tgtttgacct gccattcaac ccggatctgc tggagcagcg
    22681 tattggtcgt ctggatcgta tcggccaggc gcacgatatt cagatccatg tgccttatct
    22741 ggagaaaacc gctcagtcgg tgctggtgcg ctggtatcac gaaggtctgg atgcattcga
    22801 gcacacctgc ccgaccggac gcactattta cgatagcgta tacaacgatc tgattaacta
    22861 tctggcttca ccggatcaaa ccgaaggctt tgacgatctg atcaaaaact gccgcgagca
    22921 gcacgaagcc ctgaaagcac aattggaaca gggccgtgac cgcctgctgg agatccattc
    22981 caacggtggc gaaaaagccc aggcactggc agaaagcatt gaagagcagg atgacgatac
    23041 caacctgatc gccttcgcca tgaacctgtt cgatattatc ggtatcaatc aggacgatcg
    23101 cggcgacaac atgatcgtgc tgacaccgtc cgatcatatg ctggtgccgg acttccctgg
    23161 cttgtcggaa gatggcatca ccatcacctt cgaccgtgaa gtggcgctgg cgcgtgaaga
    23221 tgcgcagttt attacctggg agcatccgct gatccgcaac ggtctggatc tgatcctttc
    23281 tggcgatacc ggtagcagca cgatttcact gttgaaaaac aaagcgttgc cggtaggtac
    23341 gctgttggtg gaactgattt acgtggtcga agcccaggct ccgaagcagt tgcagctcaa
    23401 ccgcttcctg ccaccgacgc cggtacgtat gctgctggat aaaaacggca acaacctggc
    23461 ggcgcaggtg gagtttgaaa cctttaaccg tcagcttaac gcggttaacc gtcacaccgg
    23521 cagcaaactg gttaacgccg tgcagcagga tgttcacgct atccttcagt tgggtgaagc
    23581 acagatcgag aaatctgccc gtgcactgat tgatgccgca cgtaacgaag ccgacgaaaa
    23641 actgtctgcc gagctgtctc gtctggaagc tctgcgtgca gtgaacccga acattcgtga
    23701 cgacgaactg accgccattg agagcaaccg tcagcaggta atggaaagcc tggatcaggc
    23761 aggctggcgt ctggatgccc tgcgtttgat cgttgtgacg catcagtaac ggagccgaaa
    23821 atggggatgg aaaactacaa tccaccgcag gaaccctggt tggttatcct gtatcaggat
    23881 gaccacatta tggtggtcaa caagccgagc ggtttgttgt ctgtgccggg tcgtctggaa
    23941 gagcacaaag acagcgtgat gacgcgcatt cagcgtgatt atccgcaggc ggaatcggtg
    24001 catcgtctgg atatggctac cagcggcgtg attgtagtgg cgctgaccaa agccgcggag
    24061 cgggagttaa aacgccagtt ccgcgagcgc gagccgaaaa agcagtatgt ggcccgcgtc
    24121 tgggggcatc catcccctgc ggaaggtctg gtagatctgc cgctgatttg cgactggcca
    24181 aaccgcccga aacagaaagt ctgttacgaa acgggtaaac ctgcgcagac ggaatatgaa
    24241 gtggtggagt atgcggcgga taacacggca agagtggtgt taaaaccgat taccgggcgt
    24301 tcgcatcaat tgcgtgtgca tatgctggcg ctgggtcatc cgattctcgg cgatcgtttt
    24361 tatgcatcac cggaagcgag agcgatggca ccgcgtttgt tgctacatgc agagatgttg
    24421 acgattaccc atccggcgta tggcaatagt atgacgttta aagcgccagc ggatttttaa
    24481 cctttcctgt acgtttatac cctcatccta agggctgcct cttgtacata aatccccgag
    24541 tccggggatt ttttgtttct gccaaactgc tttcagttgt tgcgtgtggc tacgctttcc
    24601 attcggtaat tcccttcctg ctccacagcg aatttttgaa ctttttcttc acccgttgat
    24661 cgtctctatt atttcatttt atcgggtctg ttatggtcct ttctgattgt tattcatggg
    24721 ctaattagca gtctggttgg cgatgcccat ctcggtgatc cgcgtcgtgc acgacgcctt
    24781 gcatcaccag cctcttctct agctcatcat gcaggtcttt ctaattgtga aatcatccca
    24841 cgctactgct aaggttgaag gggcgttcca gccccttcaa ccttagctta ttctggatca
    24901 ttcaataatt gcgttggcaa taatttttca gcagtacttt gtttagtagc aactccttca
    24961 gtaacaattt tttttggggt gattttatta ataataatta cctcagcata taggcgggta
    25021 gcaccgtcca cggtagcacc gtccaggata gcaccgtcca gggtagcacc gtccaggtta
    25081 gcattccacc caggtttcat gtaaaggttt tcattgctat ctattattgt ttttttacca
    25141 aagtaacaat ccctcatgtc aacgccggga aggtaagcct ctccaagatc aacgcccaag
    25201 ttacctgggc ctttatgcag ctgcaacgtc tgagtacata tctccttatt ttcaatgaaa
    25261 aaagtgacac ttaactcgat atcaccgtag atctcggcga cccagcgcat cttgcccttt
    25321 gcgaccatct catccggatt ttttgcgcat gttttgagtt cattgaataa ttccagacta
    25381 tggataaact tttctttctc attctcgttg acagccccag taaagtattt tgaggcgttg
    25441 atgttgccag cctttttatt cacgaaattg aacagaagtt tataagcctc agtctgttta
    25501 tctgaccaga acagatgacg taacgtctcc cacaagcgtt gaaagatatt catcttcacg
    25561 tccatcttgt ccccgctcac catcttataa atatcgttat tgctcatatt taaaagtgta
    25621 gccggcatgc ctggaactga tactttcatg gtaatagcct cgcattgagt taaaaactca
    25681 tgatgcgcgc tctgaataaa tatactttca aaaatcgctc attcagatat gtgtttttaa
    25741 tttttaagac gtaaaaatat aattccgctc gtcgtaaagc tctcaacctt aagcagggtc
    25801 aacgcgataa aaccaacaga aaccctcccg taaaccgtgt attgagcctg tccaccactc
    25861 tgagtaaacg ccttttctca aagaagtgac cgccagggcg gccaccttta atcgtgggca
    25921 gttactcagc atcatttaca gggtcattta aaccctttct gctgttttat cagctcatag
    25981 gcctgctgaa tttcctgcgc tttctgcttc gccatctcca tcatctctgg cggcaaacct
    26041 ttcgccacca gcttatcggg atggtgttca ctcatcagct tacggtaggc acgtttgatg
    26101 gtggtcgcat catccgtcgg cttcacgccc agcacattac aggcatcttc cagcgtcggg
    26161 ccacgctgcg cttgctgcca gttaccaccg ccagattgct gctgataacc gccgccaaac
    26221 tgtgcaccgc cctgcatcat gcgcaaaaac tggtcaaact gggcgcggga gatgcctaat
    26281 tcttcagcaa taacatacag caccgcccgt tcattcgggt gcagtgaacc atcagcaaac
    26341 gccgcctgaa tctggatctc cagaaacata cgaattaagt caaaacgacc aaagcagaca
    26401 ctgcgaaact ggcgcatctt ttcgcgcagc gggtaattgt ctgattttcc cacccggaac
    26461 gcgttttgcg ccgcagtacg ggaagcgcca tgaaggttca ttcggtccat caactggctg
    26521 gcgatatgaa tatcagcctc cgtgacgcga cctttggatt tggttaaatg ccccatcact
    26581 tcaaaagtgg tggcaaaaaa cagcgcctga cgctcacgct ggttggcgaa ccacgccatt
    26641 ttacggctac gggctttatc aaacatatgg ccaattaaca ggcctaacac tacgccccaa
    26701 aagccgccgc ccatcagtaa ggccacggcc acgccaatga tttttcccca atactgcata
    26761 tattccccaa atcgacacac ggatatcagg gctatatccc acaatataaa ggtgctttta
    26821 ccgttttccg gctgcggtca attgtgggac atcgcctata atttgcatta tcatacctgt
    26881 cattcaatgc cgtgcctaac accactgacg ctattcggac aggattaaca ctagcgtaga
    26941 gatgacgagt acgttagtct ctgaccgttt gtcacgcgca acgttaccga tgatggaaca
    27001 ataaaatcaa cgtatgaaaa aacgtatccc cactctcctg gccaccatga ttgccaccgc
    27061 cctttatagt caacagggac tggcagccga cctcgcctca cagtgcatgt tgggcgtgcc
    27121 aagctatgac cgtcctctgg tacagggcga taccaatgac ttacccgtga ctatcaatgc
    27181 tgaccacgcg aaaggggact acccggatga cgccgtgttt actggcagcg tggatatcat
    27241 gcagggtaac agccgtctgc aggccgacga agtgcagctc catcaaaaag aggcaccagg
    27301 acaaccggag ccggtacgta ccgttgatgc gctcggtaat gtccattacg acgataacca
    27361 ggtgatcctc aaagggccga aaggctgggc gaatctgaac accaaagata ccaatgtctg
    27421 ggaaggtgat taccagatgg tgggtcgtca gggccgcggt aaagcggacc tgatgaaaca
    27481 acgtggcgaa aaccgctata ccattctgga taacggtagc tttacctcct gtctgccggg
    27541 ttctgacacc tggagcgtgg taggtagcga aattattcat gaccgcgaag aacaagttgc
    27601 ggagatctgg aacgcccgct ttaaggtggg tccggtaccg atcttttata gcccctattt
    27661 gcagttgccg gtgggtgaca aacgtcgctc tggtttcttg atcccgaacg ccaagtacac
    27721 caccaccaac tactttgagt tctaccttcc atattactgg aacatcgcgc caaatatgga
    27781 tgccaccatc acgccgcatt atatgcatcg tcgtggcaat atcatgtggg agaacgaatt
    27841 ccgctacctc tcccaggcgg gcgctggctt gatggaactg gactatctgc cttcagataa
    27901 agtctatgaa gatgaacacc cgaacgatga cagttcacgt cgttggttat tctactggaa
    27961 ccactccggg gtcatggatc aggtgtggcg tttcaacgtc gactacacca aggtcagcga
    28021 tcctagctac ttcaatgatt tcgataacaa gtacggttcc agtactgacg gctacgcaac
    28081 gcaaaaattc agcgttggct atgcggtgca aaacttcaat gccaccgttt caaccaagca
    28141 gttccaggtc ttcagcgagc agaacaccag tagctactcg gcagagccgc agttagacgt
    28201 taactactac cagaatgatg ttggtccgtt tgatacgcgt atttacggcc aggcagtgca
    28261 ctttgttaac accagagacg acatgcctga agcaacccgt gttcacctgg aaccgaccat
    28321 caatttgccg ctctctaata actggggcag catcaatacc gaagcgaagt tgctggcaac
    28381 ccattatcag caaactaatc ttgactggta taactccaga aacacaacca agctggacga
    28441 atccgttaac cgcgtaatgc cgcaatttaa agttgacggc aaaatggtct ttgaacgcga
    28501 tatggaaatg ctggctccgg gttataccca aacgctggaa ccgcgcgcgc agtatttata
    28561 cgtgccgtat cgcgatcaga gcgacatcta taactacgac tcgtctctgc tgcaatctga
    28621 ctactctggc ctgttccggg accggactta cggcggtctt gaccgtattg cctccgctaa
    28681 ccaggtgacg accggtgtca catctcgcat atatgatgat gctgccgttg aacgttttaa
    28741 tatttccgtt ggtcaaatct actatttcac ggagtctcgc actggcgatg acaacataac
    28801 atgggagaat gacgacaaaa cgggctcact ggtgtgggca ggcgatactt actggcgtat
    28861 ctccgagcgt tggggattgc gtggcgggat tcagtacgat acacgtctgg ataacgtagc
    28921 gaccagtaac tccagcattg aataccgtcg ggatgaagac cgtctggtac agctgaatta
    28981 ccgttacgcc agcccggaat atattcaggc tacgctgcct aagtactatt ccacagctga
    29041 gcaatataag aatggtattt cgcaggtagg tgccgtcgcc agctggccaa ttgctgatcg
    29101 ttggtcaatt gttggggcct actactacga caccaatgcg aacaagcaag ccgactctat
    29161 gttaggtgtg caatacagct cctgctgcta tgcaattcgc gtcggttacg agcggaagct
    29221 gaacggttgg gataacgata aacaacatgc ggtatatgac aacgcaatcg gctttaacat
    29281 cgaacttcgc ggcctgagct ccaactacgg tctgggtacg caagagatgc tgcgttcgaa
    29341 cattctgccg tatcaaaaca ctttgtgatt tgttgattta ccacgtaatc cgcagtgcgg
    29401 ttaattgaaa tggaaaaagt atgaagaact ggaaaacgct gcttctcggt atcgccatga
    29461 tcgcgaatac cagtttcgct gccccccagg tagtcgataa agtcgcagcc gtcgtcaata
    29521 acggcgtcgt gctggaaagc gacgttgatg gattaatgca gtcggtaaaa ctgaacgctg
    29581 ctcaggcaag gcagcaactt cctgatgacg cgacgctgcg ccaccaaatc atggaacgtt
    29641 tgatcatgga tcaaatcatc ctgcagatgg ggcagaaaat gggagtgaaa atctccgatg
    29701 agcagctgga tcaggcgatt gctaacatcg cgaaacagaa caacatgacg ctggatcaga
    29761 tgcgcagccg tctggcttac gatggtctga actacaacac ctatcgtaac cagatccgca
    29821 aagagatgat tatctctgaa gtgcgtaaca acgaggtgcg tcgtcgcatc accatcctgc
    29881 cgcaggaagt cgaatccctg gcgcagcagg tgggtaacca aaacgacgcc agcactgagt
    29941 tgaacctgag ccacatcctg atcccgctgc cggaaaaccc gacttctgat caagtgaacg
    30001 aagcggaaag ccaggcgcgt gccattgtcg atcaggcgcg taacggcgct gatttcggta
    30061 agctggcgat tgctcattct gccgaccagc aggcactgaa cggcggccag atgggctggg
    30121 gccgtattca ggagttgccg gggatcttcg cccaggcatt aagcaccgcg aagaaaggcg
    30181 acattgttgg cccaattcgt tccggcgttg gcttccatat tctgaaagtt aacgacctgc
    30241 gcggcgaaag caaaaatatc tcggtgaccg aagttcatgc tcgccatatt ctgctgaaac
    30301 cgtcgccgat catgactgac gaacaggccc gcgtgaaact ggaacagatt gctgccgata
    30361 tcaagagtgg taaaacgact tttgctgccg cagcgaaaga gttctctcag gatccaggct
    30421 ctgctaacca gggcggtgat ctcggctggg ctacaccaga tattttcgat ccggccttcc
    30481 gtgacgccct gacccgcctg aacaaaggtc aaatgagtgc accggttcac tcttcattcg
    30541 gctggcattt aatcgaactg ctggataccc gtaatgtcga taaaaccgac gcggcacaga
    30601 aagatcgtgc gtaccgcatg ctgatgaacc gtaagttctc ggaagaagca gcaagctgga
    30661 tgcaggaaca acgtgccagc gcctacgtta aaatcctgag caactaatgg ttaaaaccca
    30721 acgtgttgtg atcactcccg gcgagcccgc cgggattggc ccggacttag ttgtccagct
    30781 tgcacagcgt gagtggccgg tcgaactggt tgtttgtgcc gatgccactc tccttaccaa
    30841 ccgggcagcg atgctcggtt tgccgctcac cctccgccct tattccccca actcccctgc
    30901 acaaccgcaa actgcgggca cattaacgct acttcctgtc gcgctacgtg aatctgtcac
    30961 tgcggggcag ttagcggttg aaaatgggca ttatgtggtg gaaacgctgg cgcgagcgtg
    31021 tgatggctgt ctgaacggtg aatttgctgc gctgatcaca ggccccgtgc ataaaggcgt
    31081 cattaacgac gcaggcattc cgtttaccgg tcataccgag tttttcgaag agcgttcgca
    31141 ggcgaaaaaa gtggtgatga tgctggcgac cgaagaactt cgcgtggcgc tggcaacgac
    31201 gcatttaccg ctgcgcgata tcgcagatgc tatcacccct gcgcttttgc acgaagtgat
    31261 tgctattttg catcacgatt tgcggaccaa atttggtatt gccgaaccgc gcattctggt
    31321 ctgcgggctg aatccgcacg cgggcgaagg cggtcatatg ggtacggaag agatagacac
    31381 cattattccg gtgctcgacg agctgcgggc gcaggggatg aaactcaacg ggccgctgcc
    31441 tgccgatacc ctgtttcagc cgaaatatct cgataacgcc gacgccgtgc tggcgatgta
    31501 ccacgatcag ggtcttcccg tgctaaaata ccagggcttc gggcgcggtg tgaacattac
    31561 gctgggcctg ccctttattc gcacatcagt ggaccacggc accgcgcttg aactggcggg
    31621 acgtggcaaa gccgatgtcg gcagttttat tacggcgctt aatctcgcca tcaaaatgat
    31681 tgttaacacc caatgaataa tcgagtccac cagggccact tagcccgtaa acgcttcggg
    31741 caaaactttc tcaacgatca gttcgtgatc gacagcattg tttctgccat taacccgcag
    31801 aagggccagg cgatggtcga aatcggcccc ggtctggcgg cattgaccga accggtcggc
    31861 gaacgtctgg ataagctcac ggtcatcgaa cttgaccgcg atctggcggc acgtctgcaa
    31921 acgcatccat tcttaggccc gaaactgact atttatcagc aggatgcgat gacctttaac
    31981 tttggtgaac tggccgagaa aatgggtcag ccgctgcgtg ttttcggcaa cctgccttat
    32041 aacatctcca cgccgttgat gttccatctg tttagctata ctgatgccat tgccgacatg
    32101 cactttatgt tgcaaaaaga ggtggtgaat cgtctggttg caggaccgaa cagcaaagcg
    32161 tatggtcgat taagcgtcat ggcgcaatac tattgcaacg tgatcccagt gctggaagta
    32221 ccgccgtcag cctttacacc accacccaaa gtggattccg ccgtcgtgcg cctggttcct
    32281 catgcaacga tgcctcaccc ggttaaagat gttcgcgtgt tgagccgcat caccaccgaa
    32341 gcctttaacc agcgtcgtaa aaccattcgt aacagcctcg gcaacctgtt tagcgtcgag
    32401 gtgttaaccg gaatggggat cgacccggcg atgcgagcgg aaaatatctc tgtcgcgcaa
    32461 tattgccaga tggcgaacta tctggcggag aacgcgcctt tgcaggagag ttaacgatga
    32521 tcaattcgcc ccgagtgtgt attcaggttc aaagcgtcta cattgaggca caatcttcac
    32581 ctgataatga acgttacgtt tttgcttata ccgtaaccat acgcaatctg gggcgagcgc
    32641 cagtgcagtt gttggggcgt tactggctga tcaccaatgg caatggccgt gaaaccgaag
    32701 tccagggcga aggagtggtt ggcgtccagc cacttatcgc gcctggcgaa gagtaccagt
    32761 acaccagcgg tgcaatcatt gaaaccccgc tgggcaccat gcagggtcac tacgaaatga
    32821 tcgatgaaaa tggcgtccct ttcagcatcg acattcccgt attccgactc gccgttccca
    32881 cactcattca ttaaaagaat atggcgacat accttattgg cgacgttcat ggttgttacg
    32941 atgaactgat cgcattgctg cataaagtag aatttacccc tgggaaagat accctctggc
    33001 tgacgggcga tctggtcgcg cgcggccccg gttcactgga tgttctgcgc tatgtgaaat
    33061 ccttaggcga cagcgtacgt ctggtgctgg gcaatcacga tctgcatctg ctggcggtat
    33121 tcgccgggat cagccgcaat aaaccgaaag atcgcctgac accgctgctg gaagctccgg
    33181 atgccgacga gctgcttaac tggctgcgtc gccagcctct gctgcaaatc gacgaagaga
    33241 aaaagttggt gatggcccac gccgggatca cgccgcagtg ggatctgcag accgccaaag
    33301 agtgcgcgcg cgatgtagaa gcggtgctgt cgagtgactc ctatcccttc ttccttgatg
    33361 ccatgtacgg cgatatgcca aataactggt caccggaatt gcgggggctg ggaagactgc
    33421 ggtttatcac caatgcattt acccggatgc gtttttgctt cccgaacggg caactggata
    33481 tgtacagcaa agaatcgccg gaagaagccc ctgccccact gaaaccgtgg tttgcgattc
    33541 ctggccctgt cgctgaagag tacagcatcg cctttggtca ctgggcatcg ctggaaggca
    33601 aaggtacgcc ggaaggtatt tacgcgctgg atactggctg ctgctggggt ggtacattaa
    33661 cctgcctgcg ctgggaagat aaacagtatt ttgtccagcc gtcgaaccgg cataaagata
    33721 tgggtgaggg agaggcggcc gcgtcttaaa tcccgtcgag cataaaatct aatgctcctt
    33781 taatcaacgt tcggtgcgcg ctggcatcgc aaaatttcgt tcccagcgcg ttaactggag
    33841 cagttgcagt ctcatgcgtc atgagccgtc taccagtaaa aggattgtgt taagccgttc
    33901 tggagggcga atacggataa atcgttcaat aggtgttaat ggaataacaa tactgcgatt
    33961 aatctcacca ataatgtcgc tggtaacatc gataacaaag ggaaacgcgg cgttactgcc
    34021 gcgactgcgg tataccgtaa attgcatcgc tagaagctcc ggtactcatc agagaaacat
    34081 ccggtttcat cagccaattg atttaacgca gcgatagctt ctgcgttctc ttcacgccaa
    34141 cgtgttgctg catgtttttt aagttcagca ttgagcgcaa ccgtaagggt ggcgctcata
    34201 tttaagcctg catcacgagc gcgattgact aactcacggt cgacggtgac ggtcacactt
    34261 tgtgtggtac gttttgcagt catgatatcg tgctcctgta caatttatac gtctagtaaa
    34321 cacggatatc tgcacgtatc agtgatgccg gaattctaat atacaaactt accgccgctc
    34381 cagaatctca aagcaatagc tatgagagtt ctgcgcatca gcgtcgtgga attcgctgaa
    34441 taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt ccacttctgc
    34501 gtcgatatgc gtcagataca gtttttgcgc tttgggcagg aactgctcat aaacgcgacc
    34561 accgccaatc accatgattt ctggtacgtc accacacgcc gcgatggctt catcaaccga
    34621 tttcacccac gtaacgcgat cgtcagtacc cggttgactg ctgaggataa tatttttgcg
    34681 tcctggcagc ggacgaccga tggattccca ggtatggcgg cccataatta cgggtttatt
    34741 taaagtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca tggcgttttc
    34801 catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg agatttcccg
    34861 ataaaaaaaa ttgtcgccat tatacgtaaa gcgtaaaccg tcgtcgactg gtgcgaggat
    34921 gatgttgagg aaaattttat attctgctgg cgagtccacg ctctctccct ggactcgccg
    34981 cattacaatg aaacaaaaac aaacagttag ctgtaaagtg tgatttacgt cactctttat
    35041 taggatgagg gtttcgtttc cggttcatcc gccatgttgc cggtatgttt accttcttct
    35101 gttccctgcc agccatgtcg ttgaattaat gacagatgtt cgcggtcctc ggtaatgatc
    35161 tcacttaaca tcgcgctggt gcgtttatag accgccgcgc gggctttggt gtcgttctca
    35221 accattgcca tctcttccac catctgaata ttaaagcggc ggaacacatc ggcacgttct
    35281 cgcgcttcat acggccccag acctaaactt tccagtgcca gacgcccggt tttcagcgca
    35341 ccttcaaagg tttctcgctc tggcttttca acgcctgcct gacgcaaacg aatgtagtga
    35401 tcgacatcgc gggcgcgggc aataatctgc aaatgcggga aatgttcttt caccatctcc
    35461 gtcagttgca ggttggtttg cggatcgtcg atggcgttaa tcagcacttc cgctttcgcc
    35521 gctccggcag attccagtaa atccatccgc gtggcatcgc cataaaacac tttcatacca
    35581 aatttacgca aggtttcgat atggtccgga tcgtgatcga gcaccaccat tttcaccccg
    35641 ctggagagca gtaaacgtcc ggtgatctgc ccaaaacgac cgaatccggc aatgattacc
    35701 cgcggctgtt cttcgtcgat ctcatcggct tcacgcgcct cttcagtaga agattgctca
    35761 aggcgattga ggatcaccag cagaatcggc gttgccgcca tcgacagcgc caccgccagg
    35821 gtcagcgatt tcgcccactc cggctccagc acattcgcca tctgcgccgc accaaatacc
    35881 acaaaggcaa actcactacc ctgccctaac aacaccgcaa accagcgacg ctgtttattt
    35941 ggcacctgca acggtcgggc aatcagccac aacatggcga ttttgatgat gaggaaaccg
    36001 agcagcaaaa tgacaatgcg caatggattt tcaagcagcg tgccaaagtc tatcgacatg
    36061 ccaacaccga tgaaaaacag ccccagcagc aaacctttaa acggttcgat atcgctttcc
    36121 agcgcatgac ggtattccga gcttgccagc agtacgcccg ccagaaacgc gcccatcgcc
    36181 atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa acacgaggaa taacgccacg
    36241 gcactaaaca cttcccgcaa gccagagcgg gcaacaaaac gcagcgccgg acgcgtgaca
    36301 tagcgcccca gcaataccac cagcaccagc gcacccgcca cttttaacgc cgagaaagca
    36361 aatgcgccca tcgtcgtcga ggcactgctc gctgccagta gcgggatcat cgccaccagc
    36421 gggatcgccg cgatatcctg gaacagcagc accgcaaagg cactgcgacc catttgcgtc
    36481 accatcagat tgcgttcatt catcgcctgc atggcaatcg ccgtagagga gagcgccagc
    36541 gtcatgccga tcaattccgc gacctgccag cgcagtccaa gcaacataca gaacagcccc
    36601 agcaggccgc cgcaaatcac catctgcaat gcgccgccgc cgaacaccgc cgcacgcagc
    36661 ttccacagcc tttgtggatc gagttcgagg ccgataataa acagcatcag caccacgcca
    36721 atctcggcaa agtgcagaat agattcggca tcggtcacca gtcgcagccc ccacgggcca
    36781 ataatgcagc cagcgatcag gtagccaagt accgatccca gaccaagacg taccgcaatg
    36841 ggtacaatca gcgccgccga accgagataa atcagcgcct gaatcagcgt atggctatcc
    36901 atgatgtgcc tcctgccatt ccagcagacg ttgcttatag tgacgcgcct gcccttcgag
    36961 ggtttcgtcg tcacaaataa aggtgcagtg catggcaaac ggtggcagcc agttcagccc
    37021 gcagtagatt gccgtcgcct gtagcggctg tgacagcaca tcaaagcccg gatgcgcacc
    37081 aatttcaaaa tggctttccc cgccgccggt cgtcaccgcc cacagcaaat gtttgccatg
    37141 cagcgccgtg ccgccgtgac cgtaagccca gccgtgggag aagactttat cgatccaaag
    37201 tttaagaagc ggaggaatgc tgtaccactg catcgggtgc tgccagacga tcagatcggc
    37261 gcgagacagc gcctcctgct cggcggcaat atcgatattg aagtcaggat agagttgata
    37321 aagagagcga atttcgacgc cttccagcgt ccttgcctgt tcaagcatcc gtttattcgc
    37381 atgggaatga tgcggatacg gatgcgcata aattataaga atcataggta gcccgcgtga
    37441 ttacgccttt gttttcccac aaagtgtagt catatattca cgcggcttat agtcaataat
    37501 attgattcag aaaagagaga aatctgaaca aattacccca ttctctgccc gcggatcgtc
    37561 atattgctgg tctgcgccag tgacatccct cgagtctccg gggcaaacgc tacggaaatc
    37621 aacaagccaa acagcgagat acccgccccc attaacatcg tgttactgat accgtaatta
    37681 ttgataaaga tcggcagtgc ccaggtcgaa acaatagtgc caatacggct taaggacata
    37741 atcacgccca cggcagaggc gcggatatcc gtcgggaaga gctcattagg atagagccac
    37801 tgcaaattac ccggcccgcc agagaaaaag gcatacaccg caaaggccat cactaccagc
    37861 cagatcccca tatccgggat cagccccaaa accgccagcg ccagcgtcat catggcaaag
    37921 ctgccaatca acaatggacg ccgtccggca gtgtttaacc acagcatcgg cggaatacag
    37981 ccgagcataa agaacaggct aatcaccacg ttccccagcg ccgcgttttt gccaacaccc
    38041 aatcccaaca aaccaacgat ttgcgggcca aaggtgtaaa tggcgaacat tgggatcacc
    38101 tggcaggtcc agatggcggc aacaaacaga acaaaaggaa aatggcggcg attaaacaga
    38161 tcgcgaaaac gggtttgctg cggctgctct tcatcgaaag ccaccggttc gccaaacagt
    38221 ttgatcatca tctcttcgca ctcttttact cgccctttgc gtaacaacca gcggggagat
    38281 tcaggcagtt cgaatcgacc aatcaaaatc aacaaacagg ggatcgctgc gctacccagc
    38341 atccagcgcc aaccaccttc cacatcataa agccagtaac cgaccagatc ggcgcaggtc
    38401 gcgccgacat accacatggc agcgataaag ctgatggaaa acgcccgctg acgggtactg
    38461 gagaactcag tgatcatcga agtggcgatg ggataatctg caccgatgac aatgccgatg
    38521 agtacccgca tcactaacag ttcgacgggg gatgagacaa acatcgtcgc caccgatatc
    38581 acgccgatgg cgatgatatc gatgaggaac attttgcgcc gtccgacttt atcagagata
    38641 tagccaaaca gcgatgtgcc aacgaacagc ccggcgagcg ttcccgcgcc cagcaagcca
    38701 atccagtcag cgtccagttt cagcgccgga gtcagttgct ccagcgccac gccaatcatt
    38761 accagtacat aaccatccag aaacggacca ccgcttcccc acagcaaaat gcggcggtga
    38821 atagaagaga atttaagatc gtcaaagttt ctggacggtt gcatgggtta ttcctgtttt
    38881 tttgcaatga gagtcagtac ggcgttacgt atcaaaccaa catcagccgt aacggaactc
    38941 cacaccaaag gtgccgcgcg ggtattccca ctgttccagc gccgacccca gccccagaat
    39001 gcgacaggtg ccgcactcca gacatccggc gtaatcgaag cgcacactgc cgtcatcctg
    39061 cttcttgtac agacctgcgg ggcacgcttt caccagcagt tccagcgcct gtttatcggc
    39121 atcggccttc acaataatgt gcggatgctc ttcatcgaca ttgaatttat tgacgcccag
    39181 tttgacgtcc acattgacgg gagaagtcat aaaacggtca ctcctttcat gccatccttg
    39241 atcagattga tgaagcccac tttcttgccg tggcggagga ttttcttgcg catcagttcc
    39301 ggcgcactgc cgtcaatggt gaacaggtca cgcgccacgc ccaccgccag ttccgggtag
    39361 ccgctaaaca tgcgtgggtt atcaaggaac gccggtagtt tctggtacat acgcatatcg
    39421 cgcagcgggc cactctcaag atgctgacga tattccgcca gtttttgctt actgaaatcg
    39481 tcgcttttca tcgctgaaag cacggttttt gctgcggctt ccccggcggc aatcgccaga
    39541 tccataccgc gaatggtaaa accgaggttc atacacattc cggcggcatc accggcaatc
    39601 agtacgccgt caccgaccag ctccggcagc atgttgatgc ctgcttccgg cactacgtga
    39661 gcggaatatt ccaccagctt gccgcctgcg atcagaggtg caacggccgg atgttgtttg
    39721 aaatcttcca gcatttgcgg caccgatttt tttgcgtcat gcagatgatg gagaccacaa
    39781 accagcccca gcgacagggt attttcattg gtataaagga agccgccgcc catcaggcca
    39841 tcggtggggg aacccgcaaa taggcaagcc gcaccctgat taccctgcaa ctgaaaacgg
    39901 tcttcaataa ccgacttcgg taactcgatc agttccttca cgccaaccgc cacatccgtc
    39961 ggtttgacgc gttttgccat ccccagcttt tcggcaagga tggaattcac cccgtcagca
    40021 aggatcaccg ttttcgcttc aatcacatcg ccatcggctt ctacaccgac gactttgcca
    40081 tcgcgctgta cgaggttatc gacacgaatc ccggtaatta actgcgcgcc cgcttcttcg
    40141 gcctgctcca tcaaccaggc atcaaattta ctgcgcaaaa cggagtaaga acgctgggat
    40201 ggcgaggatt cgtcaccgtt gcagtagtcc atagtcatcg ctgacttttc cgtcataaac
    40261 gcgagttttt catgggtgat caggcgttct acgggggcgg attcggcgaa acctggaata
    40321 atgtgttcca gactgtgggc atagagacgc ccgccagtaa cgttcttggc acctgcggaa
    40381 ttgccgcgct cgataactaa cacttgcgcc ccttcgcggg cgagcaccag tgcggcaacc
    40441 gaaccggcaa gcccagcacc aacgatgatg gcgtcaaaga tatcttcgga cataagagct
    40501 ccctgtcagc ggcagggcca aaatgcagcc ctgccagagt ggatcaacgc gctaaagctg
    40561 cggtcagtgc cggaaggatc ttcacggcgt cgccaacaat gccgtaatcc gcgtactgga
    40621 agatcggcgc atttttatct ttgttgatgg cgaaaatggt ttgcgacgcg ttagcgccaa
    40681 ccatgtgctg gatctgcccg gagatcccca ccgccaggta cagttcaggt ttcagcatca
    40741 ggttggagat accgacatat cgttcgtgct ccatccattt ttcgttttcc gccaccggac
    40801 gagaacaggc caactccgca cctatcgcct tacaaagctg ttctgccagc gcaatgttct
    40861 ctttgcttcc aatgccgcga ccgacgctga ccaccagacg ggctttgtcg agatcgacgc
    40921 tgttgctctg gcgcgcctgg gttgccgtgc gggtaatcgc cacagccgga gcctgccact
    40981 ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg agccgcatca aacgtgccgc
    41041 tgctgatggt cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc gccaggccgc
    41101 cgtaaaccat gtgtttcact gtcgctttac cgtcctgtac gcagacggtg ctggcatcgt
    41161 tagacaccgc cgctttaagg cgataaccca gttttgccgc cagtaatttg ccgcgacggg
    41221 tattcggcag cagcaccagg ccgtctgcgc cgtgctggcg aatagtgtca gccatgacac
    41281 cggcgtaatc ttcgatcatc cgctcgtccg gtttgccgtt taatttccag acatgattag
    41341 caccgagctg gattgcctgt gcgccgtcgg catcattgag gacaaaggtg ttgatttgat
    41401 tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg agaaggggta tcgctgaata
    41461 cccagacttg agaaaacgtg ttcatagcat cccctgtaat taaatgactt tgcgaagatt
    41521 ttcagcaaac gcgacgatct gttcttcgcc gtcgccttca atcacgatgc gctgacgctc
    41581 gcgctgtttc ggtgcggcaa cctgttgttc agaccaggct tccacggcgt taaagccgat
    41641 atccgccgct gaccatactt ggactggctt tttcgccgcg ccgagaatgg ctttcatcga
    41701 aggaatttgt ggggagttga tatcagtgga aacagcaaca accgcaggca gcggaatgct
    41761 taaggtttct gtttcatctt ccagttcgcg ctcaacggtg agggtatctg ccgtcaggga
    41821 gataattttg ctgacgccgt taactgccgg aatattgagg atttcgccca ccagcaaacc
    41881 aacctgctgg gcataaaggt cggaagaacc atcgccacag aggatcaaat caaagccagc
    41941 tttctgtgcc gcggcagcca gtgcagtcgc cgtttgttgc ggcagtgcct gctcgaactg
    42001 gtcatcaatc accacaatca gttcatccgg gccgcgcgat agcacatctt tacgcccttt
    42061 ggcgttggtc agggctttac cgcccacact taaggccgtc acctgcccct ctgctgcctg
    42121 ttgctttagc tggcaagccg cttcaatagc gttgagatcg tattggctta ttttggcatc
    42181 ggctttactg aagtctaacg aaccatcagc attattgacc gcaatatcct gttcatcagg
    42241 cacgcactta tagcaagtaa taatcttcat tgcatctcca gaaatcatga aggaaacgtc
    42301 gttcatatcg gtggtgttaa ttacagatat cacgcccttt aatttggaaa taaaacttta
    42361 atcaccaata ttgaaaatgt cacacgcaga taaatccaac tttcaatatt gttaagtccc
    42421 tcaccaatat tgaaaacatg gcgtagcaaa aagaaatttt caatattgct ttatggatca
    42481 ccaatattga aagtcagatg ctattttttt actcacaatc aaactcatca cattgataaa
    42541 caatgaatat ttacacaaag acgccctgtt tttacccgat aaaaaactgt gactaacatc
    42601 acagaacgca gccaattgaa tacccattat gag
//
LOCUS       DACBVI010000022        40567 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.022, whole genome shotgun sequence.
ACCESSION   DACBVI010000022 DACBVI010000000
VERSION     DACBVI010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.022"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(59..340)
                     /locus_tag="IU325_06025"
     CDS             complement(59..340)
                     /locus_tag="IU325_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2574 family protein"
                     /protein_id="HAP5808599.1"
                     /translation="MNKYWLSGIIFLAYGMASPAFSSQTATLTINGRISPPTCSMAMV
                     NGQPQQYCGQLTYDVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(603..1712)
                     /gene="apbC"
                     /locus_tag="IU325_06030"
     CDS             complement(603..1712)
                     /gene="apbC"
                     /locus_tag="IU325_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HAP5808600.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1844..3877
                     /gene="metG"
                     /locus_tag="IU325_06035"
     CDS             1844..3877
                     /gene="metG"
                     /locus_tag="IU325_06035"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP5808601.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGNTT
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWHGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4018..7815
                     /locus_tag="IU325_06040"
     CDS             4018..7815
                     /locus_tag="IU325_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HAP5808602.1"
                     /translation="MRHFIFQDEKSHKFWAVEQQGNELHICWGKVGTKGQNQVKSFAD
                     AAAAAKAELKLIAEKTKKGYVENALANVHIPPITKATPEVETSPESKNKRPWVADDAV
                     IPVTGDINRFAFPHRSRPREINYLYKDGQIWKRIANNTRAYDPDNNYRSYPENWQLAF
                     AELQRRILNNQQTGSIQSDAALLWNLWNSYSTDELVDDLVIRCGLETTVEIALLALQL
                     KYKPVKADVTTIIPPDLKAESLPNWHQRLCHHLSLASEDEWQRCVDKVLAAIPSLSPA
                     RQPFAALLLPERPDIANAMALRYADQNVPAMTWLRLVVTDDAALTTAEKYDFPPLYHD
                     FRAYLAMLLANNGVRGVSRILLEFTEEHSDNPTYLFERISETEDLVKWLWKTNHPDAI
                     QILILGVIGKKKHLEYLSKACQKHPAAALAAYATLLAIHEDAQWRKALVKLITATPDL
                     VTDVIPWVNAKAAGILSECRTQLVAEECEYATADMLPELFVAPPWVINKKTNVIPVFD
                     LPVLPIPAVTDITPGITELISHTDISRFSEIAQYQSSQQTLFTDLPLIEKESWETSFI
                     PFTPEQQILWQLGFNEWLHCEDDLHEKKYIPQSAVDALLRFDFPALKAEFAKYHNNAN
                     KSWNLSALCYLPGQQAISFLNQIIIEERYSGEKEILAVFGSTAIPAFMTCLQRDHQRL
                     WIFTLFIGASELALPMAQRLQKKMAYKDAINWLANNPRHAAAGLLPLALGKPCQNREY
                     ARQAFRLLVKLNQRETIEEIGRRYNQPDVLAALATLFDSDPLEEYPAKIAPLPGFYQF
                     TLWRRPRLKSNNLPLPDDAMRHLGTMLSFPRDITSYAGLATIKETFTHESLADFGWDL
                     YTAWTEAGAPAKENWAFTSLGILGNDDTARKLTPLIRAWPGESQHKRAVYGLDVLADI
                     GSDVALMLLNGIARKIKFKALQEHAREKINIVAENRGLTMAELEDRLAPDLGLDNSGS
                     LILDFGPRQFTVGFDETLKPVVHDANGKVLKDLPKPNQSDDQTLATDSVNLFKQLKKD
                     VHAIASQQIDRLEQDMCQRRRWTAEQFRLFLVEHPLVRHLTRRVLWGVYTEENTLIAC
                     FRVAEDSTYSDAQDELFTLPAGNIGIPHVLEISPESATAFGQIYADYEKLPPFRQLDR
                     GYYHLTDNERDTHELIRWQGRLCQAGRIVGLERRGWQRLEESGSVYAMRKSTPYGALE
                     LETEHFSLIYGETGYSDLLPVESVKITSPGERYSTQPSLTFSALDAITASELINDIES
                     LFD"
     gene            7828..11622
                     /locus_tag="IU325_06045"
     CDS             7828..11622
                     /locus_tag="IU325_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HAP5808603.1"
                     /translation="MKTYIYQDEKSHKFWAVEQQGNELNISWGKVGTNGQSQVKSFAD
                     AAAAEKAELKLIAEKTKKGYVEHAENATSKHTRTVTKSLELSDFSTIIQEQPSVTPEF
                     YVADKNTDVILPWLAKDIAVVFPLEVVPTTLSHRRFPGDPVTPVDKLTQLRRLACGVS
                     QRDNKTATFDFSACTLEWQNFIAQAIDLINSPKTTKLPSPVMAVLAALELHCTRYKER
                     ADVMDQIIQEGGLEYATDVIIHLQQISIEWDYVNNNIVFLSSGISPDYLQQYSSFELR
                     LRKHLSLAEESLWQKCAQKLIAAVPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     VASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKACAAFPHAALAALAELLVQK
                     EENSWRIMLMTMLISQPTLAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAIV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPISYLTEEISNQLLAKYIWYSKHITVSHEESTA
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLISEFKVFHSPTGHYWHLGILTTL
                     PLEKAVRAWNALTLSPHTDTEYAMLHFGLKGLPGLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRQDARTWLLKYPEHAITGLLSAALGKAGEAQDNARAALRMLTEN
                     GHQPLLQEIARRYNQPEVTDAVNAMLALDPLDNHPTKIPTLPAFYQPSIWTRPVLKAN
                     AQSLPDSALQHLGEMLRFHQEEALYPGLLQVKDACTADSLAEFAWDLFTAWQTAGAPS
                     KESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAISSDIALMQLNG
                     IAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPALGLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDVSGSRLKDLPKPNKSDDESQANDAVNRYKLLKKDARTVAAQQVAR
                     LESAMCLRRRWSPENFQLFLVEHPLVRHLTRRLIWGVYSAENQLQACFRVAEDNSYST
                     ADDDLFTLQEGDISVGIPHVLEISPTDAVAFGQLFADYELLPPFRQLERNSYALTEAE
                     RNASELTRWAGRKCPSGRVMGLANKGWIKGEPQDGGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPADLSAEQVLSKLWLWEGKAESYGWGSNSTQEAQFSVLDDITASELINDIEAL
                     FE"
     gene            11632..15264
                     /locus_tag="IU325_06050"
     CDS             11632..15264
                     /locus_tag="IU325_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HAP5808604.1"
                     /translation="MDKELPWLADNAQVELKYKKGKTPLSHRSWPGEPVPVITESLIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSVPARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFITRCYESDPLLVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESSWQRCADKLIAALPGITKVRRPFIALILPEKPEIANELVSL
                     ECPWTHFHSKEWLKVVAIDPRAVRKLERYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPTRPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAEE
                     VMPWLSTQARSVLNSYLSAPPKTVIDSTDNSQMPEILVSPPWRSKKKMTVPRLDLAPF
                     ELTPQVYWQPGERERLAATESARYFSTESLAERMEHKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHVEVLINIWEND
                     SYSRYRREKSIWNLYLLAQLPREIALTFWLRINEKKHLFAGEDYFLSIFGLDALPGLL
                     LAFSYRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKSSDNSEAALLALRLLYEQGHGELLQTAANRWQRTDVWPALEHLLKQSPIEIYPT
                     RIPKAPDFWQPAMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTQMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEQLADRL
                     VPTLGLDDPQALIFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLETALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNEQDEPIDLPADALIGIAHPLEMAAEMRSEFAQLF
                     ADYEIMPPFRQLARRTVLLTPDESASNSLNRWEGKSATVGQLMGMRYKGWESGYENAF
                     VYDLGEYRLVLKFSSGFNHYNVDSKALMSFRSLHVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            15325..15645
                     /locus_tag="IU325_06055"
     CDS             15325..15645
                     /locus_tag="IU325_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808605.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLHPTFHQRDREDVDPLINDALIYIRDEFIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIEK"
     gene            16221..17298
                     /locus_tag="IU325_06060"
                     /pseudo
     CDS             16221..17298
                     /locus_tag="IU325_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
     gene            17309..19588
                     /locus_tag="IU325_06065"
                     /pseudo
     CDS             17309..19588
                     /locus_tag="IU325_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19581..20717
                     /locus_tag="IU325_06070"
     CDS             19581..20717
                     /locus_tag="IU325_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP5808606.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGCSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSDQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDHDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            20714..22714
                     /locus_tag="IU325_06075"
     CDS             20714..22714
                     /locus_tag="IU325_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808607.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCATTQTTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCTPSEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAEFTHLIWQMRSEHVTSSDDPFANDEG
                     DACRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNNRLNCAQEMARRDSVGEVPPMPWRTVVGAGIAGEAKL
                     DHLRLVSLGMRCWQDIEQYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTAV
                     VFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            22839..23300
                     /locus_tag="IU325_06080"
     CDS             22839..23300
                     /locus_tag="IU325_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1307 domain-containing protein"
                     /protein_id="HAP5808608.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(23342..23812)
                     /locus_tag="IU325_06085"
     CDS             complement(23342..23812)
                     /locus_tag="IU325_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP5808609.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATTEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(23859..24578)
                     /gene="yehT"
                     /locus_tag="IU325_06090"
     CDS             complement(23859..24578)
                     /gene="yehT"
                     /locus_tag="IU325_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HAP5808610.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRLERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(24575..26260)
                     /gene="btsS"
                     /locus_tag="IU325_06095"
     CDS             complement(24575..26260)
                     /gene="btsS"
                     /locus_tag="IU325_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HAP5808611.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            26482..27213
                     /gene="mlrA"
                     /locus_tag="IU325_06100"
     CDS             26482..27213
                     /gene="mlrA"
                     /locus_tag="IU325_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HAP5808612.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIHDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            27273..27380
                     /locus_tag="IU325_06105"
     CDS             27273..27380
                     /locus_tag="IU325_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HAP5808613.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(27361..28092)
                     /gene="yehW"
                     /locus_tag="IU325_06110"
     CDS             complement(27361..28092)
                     /gene="yehW"
                     /locus_tag="IU325_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HAP5808614.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIIVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(28097..29023)
                     /gene="yehX"
                     /locus_tag="IU325_06115"
     CDS             complement(28097..29023)
                     /gene="yehX"
                     /locus_tag="IU325_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HAP5808615.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLEPNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGHSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(29016..30173)
                     /gene="yehY"
                     /locus_tag="IU325_06120"
     CDS             complement(29016..30173)
                     /gene="yehY"
                     /locus_tag="IU325_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HAP5808616.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWMTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGVWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAGLVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(30180..31097)
                     /gene="osmF"
                     /locus_tag="IU325_06125"
     CDS             complement(30180..31097)
                     /gene="osmF"
                     /locus_tag="IU325_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HAP5808617.1"
                     /translation="MPLSKLWAGSLVLLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDEKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(31246..33543)
                     /gene="bglX"
                     /locus_tag="IU325_06130"
     CDS             complement(31246..33543)
                     /gene="bglX"
                     /locus_tag="IU325_06130"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HAP5808618.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLEAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDATRHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            33739..35454
                     /locus_tag="IU325_06135"
     CDS             33739..35454
                     /locus_tag="IU325_06135"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAP5808619.1"
                     /translation="MSSMTTTDNKAFLNELARLVGSSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(35493..36425)
                     /locus_tag="IU325_06140"
     CDS             complement(35493..36425)
                     /locus_tag="IU325_06140"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HAP5808620.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(36599..37186)
                     /locus_tag="IU325_06145"
     CDS             complement(36599..37186)
                     /locus_tag="IU325_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1282 domain-containing protein"
                     /protein_id="HAP5808621.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     DGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            37356..37934
                     /locus_tag="IU325_06150"
     CDS             37356..37934
                     /locus_tag="IU325_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAP5808622.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(38053..38814)
                     /locus_tag="IU325_06155"
     CDS             complement(38053..38814)
                     /locus_tag="IU325_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP5808623.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGVDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGVQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDGDVKPGAEPSIPLRRFGATHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(38867..40393)
                     /gene="mdtQ"
                     /locus_tag="IU325_06160"
     CDS             complement(38867..40393)
                     /gene="mdtQ"
                     /locus_tag="IU325_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311052.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HAP5808624.1"
                     /translation="MPLMYCHAKLKNISQHTVISAHLFLPDYSPMNRDSFYPAIACFP
                     LLLMLAGCAPMHETRQALSQQTPAVQVDTALPTALKNGWPDSQWWLEYHDNQLTSLIN
                     NALQNAPDMQVAEQRIQLAEAQAKAVATQDGPQLDFSADMERQKMSAEGLMGPFALND
                     PAAGTTGPWYTNGTFGLTAGWHLDIWGKNRAEVTARLGTVKARAAEREQTRQLLAGSV
                     ARLYWEWQTQAALNTVLQQIEKEQNTIIATDRQLYQNGITSSVEGVETDINASKTRQQ
                     LNDVAGKMKIIEARLSALTNHQTKALKLNPVALPKVASQLPDELGYSLLARRADLQAA
                     HWYVESSLSTIDAAKAAFYPDINLMAFLQQDALHLSDLFRHSAQQMGVTAGLTLPIFD
                     SGRLNANLDIAKAESNLSIASYNKAVVEAVNDVARAASQVQTLAEKNQHQAQIERDAL
                     RVVGLAQARFNAGIIAGSRVSEARIPALRERANGLLLQGQWLDASIQLTGALGGGYKR
                     "
BASE COUNT        10153 a        10770 c        10096 g         9548 t
ORIGIN      
        1 atcattcaca cgaatgattt cagctttcta aatccgatta aaaaaccagg gtggcaaatt
       61 aatcgtagcg atttaacacg attcgacgtt tgctatcgct acctgcaaca accacttctg
      121 tcgttacacc tttgactggc gaagaaacct gatgacgggt atcaacatca tacgttaact
      181 gaccacaata ctgttgaggt tgaccattaa ccatagccat actgcatgtt gggggactga
      241 tcctgccatt aatcgttaat gtggcagtct gtgaagagaa cgcgggagaa gccattccat
      301 aggcaagaaa aataattcct gacagccagt acttattcat tgaacgttat ccctgtagta
      361 aaggttatgc ctggatagaa tgagtgcata acaaactata gctgtacatc cactacacag
      421 ccacgaagga tgataatgaa gcattgcctg tacgatcaat cgactttata gagtttcgga
      481 cgaaggtccg cagaatattt gcagcattaa ataagtgttc attaatgcgg caaaaacaaa
      541 tacaccgcat caatgtaaca tctctgcggt gtatgaaaaa caaccaaaaa ggttaatacg
      601 cgttagaccg cgcggaagga aatctcgcca ggaatgactt ctccctgcca ataaagctgg
      661 gctgcaacgc ggtcagccag ctggcggtag atagcggtaa attcgctctc cggacggctg
      721 ataaccgttg gcgtgccttt atcgagatct tcacgtaagg agatgtgtaa cggcatctgc
      781 cccagcaact gcgtgtgata tttctcggcc agtttctctg caccaccggt accgaaaatc
      841 ggttcatggt gaccacagtt actgcaaata tgcacactca tgttctcgac aataccgagc
      901 accggaacct cgactttctc gaacatcaca atgccttttt tcgcatcgat caacgcaatg
      961 tcctgcggag tggtgaccac taccgcgcca gttactggta tgttttgtgc cagtgtcaat
     1021 tgaatgtcac cggtgcctgg cggcatatca agcaccagat agtcgagatc cggccacaag
     1081 gtttcctgca acatctgcat taacgccttg ctcgccatcg gaccacgcca caccatcgca
     1141 ttgtcgtcag tgaccagata accaattgaa ttggttgcca gtccatgaga cataatcggt
     1201 gccatatggg taccgtccgg cgaagttgga cgctgatttt ctgcacccag catggtgggt
     1261 attgatgggc catagatatc ggcgtccaga atgccgactt tcgccccttc cgccgccagt
     1321 gccagcgcca ggtttaccgc cgtggaggat ttacccacgc cgcctttacc ggagctgacc
     1381 gcaatgatgt tcttcacgcc gttaattccc ggctgattct tcacgcgttt taaggtggcg
     1441 atattgtgcg acagtttcca gtcgatagct ttcgccccgg tgatgcgcag cagttcggcg
     1501 ctacattgct cttttaactc ttcgaacgcg ctatgccaga cgaatggcat aaccagttcc
     1561 acatgcaacg tgtcgtccat ccaggcgaca tggtgcaacg ctttcagcgt ggtgaggtta
     1621 tgcttcaggg ttgggtgctg aaaattggcc agtgtcccgg cgaccattgc gcgcaaggct
     1681 tctggtgatt tggcctggga ttgttcgttc atcccgactc cttttttgta tagataaacc
     1741 atcagctgat agtttacctg aagaatatag agaagtactt acttaacatt tttccatttg
     1801 gtactatcta accccttttc actattaaga agtaatgcct actatgactc aagtcgcgaa
     1861 gaaaattctg gtgacgtgcg cactgccgta cgctaacggc tcaatccacc tcggccatat
     1921 gctggagcac atccaggctg atgtctgggt ccgttaccag cgaatgcgcg gccacgaggt
     1981 caacttcatc tgcgccgacg atgcccacgg cacaccgatc atgctgaaag ctcagcagct
     2041 cggtatcacc ccggagcaga tgattggcga aatgagtcag gagcatcaga ctgatttcgc
     2101 aggctttaac atcagctatg acaactatca ctcgacgcac agcgaagaga accgccagtt
     2161 gtcagaactt atctactctc gcctgaaaga aaacggtttt attaaaaacc gcaccatctc
     2221 tcagctttac gatccggaaa aaggcatgtt cctgccggac cgttttgtaa aaggcacctg
     2281 cccgaaatgt aaatccccgg atcaatacgg cgataactgc gaagtctgcg gtgcgaccta
     2341 cagcccgact gaactgatcg agccgaaatc ggtggtttct ggcgctaccc cggtaatgcg
     2401 tgattctgaa cacttcttct ttgatctgcc ctctttcagc gaaatgttgc aggcatggac
     2461 ccgcagcggt gcgttgcagg agcaggtggc aaacaaaatg caggagtggt ttgaatccgg
     2521 cctgcaacag tgggatatct cccgcgacgc cccttacttc ggttttgaaa ttccgaacgc
     2581 gccgggcaaa tatttctacg tctggctgga cgcaccgatt ggctacatgg gttccttcaa
     2641 gaatctgtgc gacaaacgcg gcaacaccac cagcttcgat gaatactgga agaaagattc
     2701 caccgccgag ctgtaccact tcatcggtaa agatatcgtt tacttccaca gcctgttctg
     2761 gcctgccatg ctggaaggca gcaacttccg caagccgact aacctgtttg ttcatggcta
     2821 tgtgacggtg aacggcgcga agatgtccaa gtctcgcggc acctttatta aagccagtac
     2881 ctggctgaat cattttgatg ctgacagcct gcgttactac tacactgcga aactctcttc
     2941 gcgcattgat gatatcgatc tcaacctgga agatttcgtt cagcgtgtga atgccgatat
     3001 cgtgaataaa gtggttaacc tggcatcccg taacgctggc tttatcaaca agcgttttga
     3061 cggcgtgctg gcaagcgaac tggctgaccc gcagttgtac aaaaccttca cggatgccgc
     3121 tgaagtgatt ggtgaagcgt gggaaagccg cgaatttggt aaagcagtac gcgaaattat
     3181 ggcgctggct gacctggcta accgctatgt cgatgaacag gctccgtggg tggtggcgaa
     3241 acaggaaggc cgcgatgccg acctgcaggc aatttgctcg atgggcatca acctgttccg
     3301 cgtgctgatg acttacctga agccggtact gccgaaactg accgagcgtg cagaagcatt
     3361 cctcaataca gaactgacct ggcatggtat ccagcaaccg ctgttgggcc ataaagtgaa
     3421 tccgttcaag gcgctgtata accgcatcga tatgaagcag gttgaagcac tggtggaagc
     3481 atctaaagaa gaagtaaaag ccgctgccgc gccggtaact ggcccgctgg ctgacgatcc
     3541 aatccaggaa actatcacct ttgatgactt cgccaaagtt gacctgcgcg tggcactgat
     3601 tgaaaacgca gagtttgttg aaggttccga caaactgctg cgcctgacgc tggatctcgg
     3661 cggtgaaaaa cgcaatgtct tctccggcat tcgttctgct tacccggatc cgcaggcact
     3721 gattggtcgt cacaccatta tggtggctaa cctggcacca cgtaaaatgc gcttcggtat
     3781 ctccgaaggc atggtgatgg ctgccggtcc tggcgggaaa gatattttcc tgctaagccc
     3841 ggatgccggt gctaaaccgg gtcatcaggt gaaataatct cctttcaagg cgctgtatcg
     3901 acagcgcctt ttctttataa attcctaaag ttgttttctt gcgattttgt ctctctctaa
     3961 cccgcataaa tactggtagc atcagcattc aactggataa aattacaggg atgcagaatg
     4021 aggcacttta tctttcagga cgaaaaatca cataaattct gggcggtgga gcaacaggga
     4081 aacgagttgc atatctgttg gggaaaagtt ggcaccaaag ggcaaaacca ggtaaaaagt
     4141 tttgcggatg ctgcggcagc ggcaaaagcg gagcttaagc tgatcgcaga gaaaacgaaa
     4201 aaaggctatg tggaaaatgc tttagcaaac gtgcatatcc cccccatcac caaagccact
     4261 cctgaagttg aaacctcccc tgagagtaaa aacaaacgcc catgggtggc tgatgatgca
     4321 gtcattccgg taactggcga tattaatcga tttgcttttc ctcatcgctc tcgccccaga
     4381 gagatcaatt atctgtacaa agatggccaa atatggaagc gcatcgcgaa taacaccagg
     4441 gcatatgacc ccgacaataa ttaccgctct tatcctgaaa actggcagct ggcgtttgcc
     4501 gagttacaaa ggcgaattct gaataatcaa caaacaggaa gtatacaatc tgatgcggca
     4561 ttgctctgga atttatggaa ctcttactct acagatgaac tggtcgatga cttagtcatt
     4621 cgctgtggtc tggaaactac cgttgaaatc gcccttctcg ctcttcaact caaatataaa
     4681 ccagtaaaag ccgacgtcac caccattatt ccccctgatc tcaaagcaga atcactaccg
     4741 aactggcatc aacgtctatg tcatcatctt tcactcgctt cagaagatga gtggcaacgc
     4801 tgtgtagata aagttctcgc tgctattccc tcgctatccc cagcacggca accttttgcg
     4861 gcgttactgc tccctgaacg cccggatatt gccaatgcta tggcgctacg ttatgcagat
     4921 caaaacgttc cggcgatgac ctggttaaga ctggtggtaa ccgatgacgc cgctctgact
     4981 acagcagaaa aatacgactt cccaccgttg tatcatgatt ttcgcgctta tctggcaatg
     5041 ttgctggcaa ataatggggt gcgcggtgta agccggatac ttcttgagtt tacggaagag
     5101 cattctgaca atcccactta tctcttcgag cgcatcagcg agactgaaga tcttgtcaaa
     5161 tggctatgga aaacgaatca cccggatgcg attcaaattc tgatcctcgg tgtaattggc
     5221 aagaaaaagc acctggaata cttaagcaaa gcctgtcaaa aacatcccgc tgcggctctt
     5281 gccgcttatg caactttact ggcaatacat gaagatgcac agtggcgtaa agcgcttgtc
     5341 aaactgatta ccgccacgcc agatttagtc actgacgtca ttccctgggt taatgctaaa
     5401 gcggcaggca tcttatctga atgtcgtact cagttagtcg ctgaagaatg tgaatacgcc
     5461 actgcggata tgttgccgga gctgtttgtc gctccaccgt gggtaataaa taagaaaacg
     5521 aatgtaatac ccgtatttga tttaccggtg cttcccatcc ctgcagtcac tgacatcacg
     5581 ccaggaatta cagagttaat cagtcatact gacatatcca gattcagtga aatcgcgcaa
     5641 tatcagtcat cacagcaaac cttatttacc gacctgccac taattgaaaa agaaagctgg
     5701 gagacatctt tcattccttt tacaccagaa caacaaatcc tttggcaatt aggttttaat
     5761 gaatggctgc attgcgaaga tgacttacat gaaaaaaaat atataccgca aagcgctgtt
     5821 gatgccttgt tgcgctttga ttttccagca ctaaaagctg agtttgcgaa atatcacaac
     5881 aatgccaaca aaagctggaa tctatctgcg ctttgttact taccgggaca acaggcgatt
     5941 tcatttctca accaaataat cattgaggag cgatattcag gtgagaaaga gattctggca
     6001 gtttttggta gtacggccat tcctgcgttt atgacctgct tgcaacgaga tcatcaacga
     6061 ctgtggatat tcaccctctt tattggggcc agtgaactag ccttaccaat ggcgcagcgg
     6121 ttacagaaaa aaatggctta caaggatgcc attaattggc tggcaaataa cccgcgccat
     6181 gccgccgccg ggctactgcc tttggcgctg ggcaaaccgt gccaaaatcg ggaatatgcc
     6241 cgccaggcat tccgcctgct agtgaaatta aaccagcgag aaacgattga agaaatcggc
     6301 cggagataca accaacctga tgttttagct gcgctggcaa cattgttcga tagcgatccg
     6361 ctggaagaat atcccgctaa aatcgcccca ctgcccggct tttatcagtt caccttatgg
     6421 cgcagaccac ggcttaaaag taacaacctg cctctgccag atgacgctat gcgccacctg
     6481 ggcacaatgt tgagctttcc acgcgacatt acctcctacg ctgggctggc gaccatcaaa
     6541 gagaccttca cccacgaatc actggctgac tttggctggg atctctacac ggcatggaca
     6601 gaagctggcg cacccgcaaa agaaaactgg gcatttactt cgcttgggat tttaggcaat
     6661 gacgacaccg cacgtaaatt aaccccgctt atccgtgcct ggccgggtga atcccagcat
     6721 aaacgcgcgg tttacgggct ggatgtatta gctgatattg gcagcgatgt tgcgctaatg
     6781 ctgcttaatg gcatcgcgcg aaaaattaaa ttcaaagcat tacaggaaca tgcccgcgag
     6841 aaaatcaaca tagttgctga aaaccgtggg ctgactatgg ctgaactgga agaccgcctg
     6901 gctccagatt tagggcttga taacagtggc tcgctgatac tggatttcgg cccacgtcaa
     6961 ttcaccgttg gttttgacga aaccttaaaa cctgtagtgc acgatgcaaa cggcaaagtc
     7021 ctgaaagatt tacccaaacc aaatcagagc gatgatcaaa cactggcaac tgactccgtt
     7081 aacctcttca aacaattgaa gaaagatgtg cacgccattg ccagccagca gattgatcgt
     7141 ctggagcagg atatgtgcca gcgcagacgc tggacggcag agcagttccg cctgtttctg
     7201 gtggagcatc cgctggtacg tcacttaacc cgacgggtgt tatggggggt atatactgag
     7261 gaaaacacgc tgatcgcctg cttccgggtc gcagaagaca gcacttacag cgatgcgcag
     7321 gatgagttat tcacgctgcc agcaggaaac atcggtatac cgcatgtgct ggaaatatct
     7381 cctgaatccg ccactgcatt tgggcaaatt tatgctgatt acgaaaagct tcctcccttt
     7441 cgccaactcg atcgtggtta ttaccacctt actgataatg aacgtgatac ccatgaattg
     7501 atacgctggc aaggacgtct ttgccaggcc gggcgcattg tcgggctgga acgtcgaggc
     7561 tggcaacgtc tggaagaaag cggcagcgtc tatgcgatgc gcaaatctac cccttatggc
     7621 gctctcgaac ttgaaacaga acatttttca ttaatttatg gcgaaacggg gtatagcgac
     7681 ctgctacctg ttgaaagcgt aaaaatcact tcaccagggg aacgctacag tacacagccc
     7741 tcgctcacgt tctccgcgct tgatgccatc accgccagcg aactcattaa cgacatcgaa
     7801 tcactttttg attaaaagga ctttgcaatg aaaacctata tttatcagga cgaaaaatca
     7861 cataaattct gggcggtgga gcagcaggga aacgaactga atatcagttg gggcaaagtc
     7921 ggcaccaacg ggcaaagtca ggtaaaaagc tttgcagatg ctgcggcggc ggaaaaagcg
     7981 gaactcaagc tgattgcgga gaaaacgaaa aaggggtatg tggaacatgc ggagaatgct
     8041 acatcgaaac atactcgaac ggtaacgaaa tctctcgaat tatctgactt ttctaccatc
     8101 attcaggaac agccctcagt tacaccagaa ttctacgtgg ctgacaaaaa tacagacgtt
     8161 attttaccgt ggctggcaaa agatattgct gtcgtttttc cgcttgaagt cgtacccacc
     8221 accttaagcc atcgccgctt tcccggagat cctgttacac ctgtagataa attaacccag
     8281 ttacgtcgct tagcctgtgg cgtgtcgcaa cgggataaca aaacagctac atttgacttc
     8341 agcgcctgta ctttagagtg gcaaaacttc atcgctcagg cgatcgatct gattaacagc
     8401 ccgaaaacga caaaattacc atcaccggta atggctgtac tcgcggcact tgaactgcac
     8461 tgcacgagat ataaagagcg tgcggatgtt atggatcaga ttattcagga aggcggtctg
     8521 gaatatgcta ctgatgtaat aatacatctt caacaaatat ctattgaatg ggattatgtc
     8581 aacaataata ttgttttcct gtcgtctggc atttcacctg attatttgca gcaatattcc
     8641 agctttgaat tacgcctacg taaacattta tcactggcgg aagagtctct ctggcaaaaa
     8701 tgtgcacaaa aacttattgc cgcagttcca catattccag aatggcggca accattaatt
     8761 gctttgttat tgcccgaaaa accagaaatt gcacatgaaa ttgcccagcg tctactgggg
     8821 caaaagaaat taccctcgct ggagtggcta aaaatagtgg cgactgatga gcacattctt
     8881 gcctcattag aaaaatatca cgagccatat gccatttttg atgattacta ttgtggtgcg
     8941 atatggtcag ccaccgtatt acaggagcaa ggtgttgcgg ccctgccccg atttgctccc
     9001 tatgtcgcaa gtgactactg cgccgatgtg ttgcgtcata tcaatcatcc gttcgcatta
     9061 acactgctta tacgtgtagc agggcatact aaacgctgtc acgatcgaat gacgaaagcc
     9121 tgcgctgcgt tcccacacgc agcactggca gcactggcgg aacttctagt gcaaaaagaa
     9181 gagaatagtt ggcgcattat gctaatgacg atgcttatct cgcaaccgac actagcagaa
     9241 caggtcattc cctggctttc gacacccgca gttgcagtgc tgaaatcatg ccagcaacaa
     9301 ctgacacagc cctcaaacca tgccagcgcc gatctactgc cggccatagt agtctcccca
     9361 ccatggcttt cgaaaaagaa aaaatcgccg attccagtgc ttgatttagc accattaggt
     9421 attgagccaa tcagttatct gacagaagaa atcagtaatc aacttttggc gaaatatatc
     9481 tggtattcaa aacacatcac ggttagtcat gaagaaagta ctgccaatct gttggcaagg
     9541 atgggttttc aacgacggat cgctggtaca tatattaaag ctcccgaagc ggtagttgag
     9601 gcatggctaa atgaagatta ttcaacttta ataagtgaat ttaaggtgtt tcattcacct
     9661 accgggcatt attggcattt ggggattttg acaacattgc cgctggagaa agcagtaaga
     9721 gcatggaatg cccttactct atctccgcat accgataccg aatacgccat gttacatttt
     9781 ggactcaaag gattacctgg gttagtaaac tcacttgcac gctatccaca ggaagccttg
     9841 cccataacga attacttcgc agcgagtgag ctggcacctg ccgtcgcccg cgccttcaac
     9901 aaactcaaaa ccctacgaca agacgcccgc acctggctgc tgaaataccc ggaacacgcc
     9961 attaccggcc tgctgtctgc ggcgctcggc aaagccggtg aagcacagga taacgcccgc
    10021 gctgcattgc gtatgcttac cgaaaacggt catcaaccat tactgcaaga aatcgcccgg
    10081 cgttataacc agccggaagt aacagatgcg gtgaacgcta tgcttgcgct cgatccctta
    10141 gataatcacc cgacaaaaat ccccactctt ccggcctttt atcagccatc gatctggacc
    10201 cgcccggtat taaaagcaaa tgcccaatca ctgcccgata gcgcactcca gcacctcggt
    10261 gaaatgctcc gcttccatca ggaggaggcg ctgtatccgg gattattgca ggtgaaagat
    10321 gcctgtaccg ccgactcact ggctgaattt gcctgggatc tgtttaccgc ctggcagacc
    10381 gctggcgcgc cgtcgaaaga gagttgggcg ttcactgcgt taggcgttct cggtaacgat
    10441 gacaccgccc gcaaactgac gccattaata cgcgcctggc ctggtgaatc ccagcataaa
    10501 cgcgccaccg tcgggctgga tatcctcgcc gctatcagta gtgatatcgc cctgatgcag
    10561 cttaacggca ttgcccagaa actgaaattc aaagcattac aggagcgggc gaaagagaaa
    10621 attgccgaca ttgccgaaag tcgcgaactg acggtagcgg agcttgaaga tcggttagca
    10681 ccggctctcg gtctggatga taacggttcg ctgctgctgg attttggccc acggcagttc
    10741 accgtcagct ttgatgaaac cttaaaaccg tttgtgcgcg atgtttccgg cagccgcctg
    10801 aaagacttac ctaaacccaa taaaagcgat gatgaatcac aggcaaacga tgctgttaac
    10861 cgctacaaat tgctgaaaaa agatgcgcgt accgtcgccg cccagcaggt agcaaggctg
    10921 gaatccgcca tgtgcctgcg ccgccgctgg tcgccggaaa actttcagct cttcctggtt
    10981 gagcatccgc tggttcgtca cttaacccgc cgtctgattt ggggcgttta tagcgccgaa
    11041 aaccagctac aggcgtgctt tagggtggcg gaagataaca gctacagcac cgctgacgat
    11101 gatcttttca ccttgcagga aggcgatatc tctgttggca ttcctcacgt tctggaaata
    11161 tcaccgacgg atgctgtcgc ctttggtcag ctttttgccg actacgaact gctaccaccg
    11221 ttccgtcagc ttgaacgtaa cagttacgcc ctgacagaag ccgagcgcaa cgccagcgaa
    11281 ctgacccgct gggcaggcag aaaatgcccg agcggccggg tcatggggct ggcaaataaa
    11341 ggctggataa agggcgaacc acaggatgga ggctggatcg gatggatgat caaacctctc
    11401 ggtcgctggt cgttaatcat ggaaatcgac gaaggttttg cggtgggtat gtcaccagcc
    11461 gatcttagcg ctgagcaggt cttaagcaag ctgtggctat gggaaggcaa agcggaaagc
    11521 tatggctggg ggagtaattc aactcaggaa gcgcagttct ccgtgctcga cgacatcacc
    11581 gccagcgaac tcattaacga tattgaagcc ctgtttgaat aaggaaagag catggacaag
    11641 gaattaccgt ggctggcgga taacgcccaa gtggaactga aatataaaaa aggcaaaacg
    11701 ccgctcagcc atcgcagttg gccgggcgaa ccggtgcctg ttatcactga aagcctcatc
    11761 cagacactgg gtgatgaatt gctacaaaaa gctgagaaga aaaaaaacat tgtctggcgt
    11821 tatgaaaatt tttcactgga gtggcagtcg gccattacgc aggccatcaa cttgattggc
    11881 gaacacaaac cctcagttcc ggcccggaca atggcggcgc tagcctgtat cgcgcaaaat
    11941 gatagccaac agttgctcga cgaaatcgtc caacaagagg gactggaata tgcgactgag
    12001 gtggtgattg cacgccagtt tattacacga tgttatgaaa gtgatcctct gctagtgaca
    12061 ttgcagtatc agaacgagga ttatggctat ggttatcgat cagaaaccta taacgaattc
    12121 gatctccgac tgcgtaagca tctctctctg gcagaggaaa gcagctggca gcgttgcgcc
    12181 gacaaactca ttgccgcact gccaggaata accaaagttc gccgcccttt tattgctctg
    12241 atcctcccgg aaaaaccaga aatcgccaat gagttggtaa gccttgaatg cccgtggact
    12301 cattttcatt ctaaggagtg gttaaaagtt gttgctattg accccagagc ggtgagaaaa
    12361 ctcgaacgct actggagcca ggatatattt agcgatcggg aagccagcta catgtcgcat
    12421 gaaaaccact tcggctacgc ggcctgcgct gcccttttgc gcgaacaagg actggcagcc
    12481 gttccacgcc tggcaatgta tgcccataaa gaagattgcg gcagtctgct ggtacaaatt
    12541 aaccatccgc aggtcatccg caccctgctg ttagttgctg ataaaaacaa acccagcctg
    12601 caacgtgtag ctaaatacag taaaaatttc ccccatgcga cgcttgccgc actggcagaa
    12661 ctgctggcgc taaaagaacc acctacgcgc cctggttatc caatcatcga agacaaaaag
    12721 ctgcctgcac agcaaaaagc acgtgatgaa tactggcgta cgctgttaca aacgctgatg
    12781 gcatcgcagc cacaactggc agaagaggtg atgccgtggt taagtactca agcccggtca
    12841 gtgctgaata gttatttatc ggcaccgcct aaaacggtta ttgatagcac cgataacagc
    12901 caaatgcctg aaatcctcgt ttcaccaccg tggcgtagta agaaaaaaat gacagttcca
    12961 cgtcttgatt tggcaccgtt cgaattaacg ccgcaagttt actggcaacc aggcgaacga
    13021 gagaggcttg ccgccactga gtctgcccgt tatttcagca cggaatctct tgcggaacgc
    13081 atggaacata aaagtggacg agttgtacta caggagctgg gttttggaga tgatgtatgg
    13141 ctgtttctga attatatact ccccggaaaa ctggatgctg cacgcaattc actcattgtt
    13201 cagtggcatt actaccaggg gcgggttgaa gagatcctga atggctggaa ctccccggaa
    13261 gcacaattag cagaacaggc gcttcgcagc ggtcacgtag aagtgttaat taacatatgg
    13321 gaaaatgaca gctactcacg ctatcgtcgg gaaaagagta tctggaacct gtatttattg
    13381 gcacagttgc cgcgtgagat agctttgact ttctggctac gtatcaatga gaaaaagcat
    13441 ctgttcgcgg gtgaggacta ttttctcagt atcttcggat tggatgctct accaggtctg
    13501 ctgttggctt tttcatatcg tccaaaagaa acatttccgt taattttaaa ttttggcgca
    13561 acagaactgg ccctgcccgt tgcccgcgtc tggcgacgtt tcgcggcgca gcgtgatctg
    13621 gctcgccagt ggattttaca atggccggaa catacggcta ctgcacttat ccctcttgtc
    13681 ttcaccaaat ccagcgataa tagcgaagct gcattacttg ccctacgttt actttacgaa
    13741 cagggacatg gtgaattgct gcaaaccgcg gcaaaccgct ggcagcgtac agatgtatgg
    13801 cctgccctgg agcatttact taaacagagt ccaatagaga tttacccgac acgcattcca
    13861 aaagcccctg atttctggca gccggcaatg tggtccaggc cgcgccttat cacgaataat
    13921 cagcctgtta ccgatgacgc actggaaatt atcggcgaaa tgctgcgctt tacccagggc
    13981 ggacgttttt atagcggact ggaacaactg aaaactttct gccagccgca aacgctggca
    14041 gcttttgcct gggatctttt caccgcgtgg caacaagctg gtgctcccgc aaaagacaac
    14101 tgggcatttc tggcgttaag tctctttggc gacgaaagca cggcacggga tctgacgaca
    14161 cagatccttg cctggccaca ggaaggcaaa tctgcccgtg ccgtcagcgg cctgaacata
    14221 cttactcaga tgaataatga tatggcactg atacagctgc atcatatatc gcaacgggcg
    14281 aaatcccgcc ccttacgtga taacgcggcg gaatttcttc aggtggtcgc agaaaatcga
    14341 gggctaagcc aggaacagtt agcggacaga ttagtcccaa cactgggtct tgatgatccg
    14401 caggcgttga tttttgattt tggtccacgg caatttaccg ttcgcttcga tgaaaacctt
    14461 aacccggtta tctttgatca gcaaaacgtt cgccagaaaa gcgttccccg gttgcgcgcc
    14521 gatgacgatc aactgaaagc acccgaggca ctggcccgac taaaagggct aaaaaaagat
    14581 gccactcagg tgagcaaaaa cctgctcccg cgtcttgaaa ctgccctgcg caccacccga
    14641 cgctggtcgc tggcagattt tcattctctg tttgttaatc atccctttac ccgtctggtt
    14701 actcagcgat taatatgggg tgtttatccg gcaaatgaac cgcgtcgttt actcaacgcc
    14761 tttcgtgtgg ccgctgaggg ggagttctgc aatgagcaag atgagccaat tgacctgcct
    14821 gccgatgctc tgattggcat tgcccacccg ttagaaatgg cagcagaaat gcgcagtgag
    14881 tttgcacagc tttttgccga ttacgaaatt atgccgcctt ttcgccaatt ggcacggcgc
    14941 acagtactgc tcacgcctga cgagtcagcc agtaatagtt tgaatcgctg ggaaggtaaa
    15001 tctgctaccg ttgggcaact tatgggaatg cgatacaaag gctgggagtc aggctatgag
    15061 aatgcatttg tctatgacct gggcgagtac cggctggtcc ttaagttttc atccggtttt
    15121 aaccactaca atgttgatag caaagcgcta atgagcttcc gttctcttca tgtataccgt
    15181 gacaataaat ctgtcacttt tgccgaactt gatgtgtttg atttgagtga agcgttaagc
    15241 gcacccgacg tcattttcca ttaaaacaca gcgggcagtg atgcgactgc ccgttattaa
    15301 cacgacctgc caccgggaaa agcgatgatc gtgcaaaaag agctggttgc tatttacgat
    15361 tatgaggtcc ctgtacctga agatccgttt tccttcagac ttgagatcca taaatgctct
    15421 gaattattta caggttccgt ctaccgactg gagcgattcc ggctacatcc aacatttcat
    15481 caacgtgatc gagaggatgt cgacccgcta ataaatgatg cgttgattta tataagagat
    15541 gagtttattg atgagcgaaa attacggggc gaatcaccag aaacggtaat agccattttt
    15601 aatcgcgaac tacagaatat attcaaccaa gaaatagaaa aatagcttta ttaaaggggg
    15661 aaacggtatt cccccttaga tgcgtttcaa tttgcccatt acttcaacaa atcaatcaac
    15721 cgtaacagag ccactaatag ctccagtacg gcaatgagca cggttaactt ttgtgtcagc
    15781 gtgtccatcc gctctcctta ttaaggagcg caacaccagc accttaccct tacactgcct
    15841 gtcgatactg gtttacgtat gttggaccac gatgttgcac tccggatcag ggcaccacct
    15901 gcagcaccac ccgtccggta gccaggactt aaaattggtc cgatccggcg caacgtccca
    15961 accgtctgga ttataaatct cattatctta attgcaacgg ggtccaccga tggttataat
    16021 tcgttcgttg gaacacgatg ttgcacaggc tgcggtgttg ggcctgaaaa tagtaaataa
    16081 aagaacctgc ctcaccagca ggttttttta tttactgtga tctgctttcc agatattttt
    16141 cgctcaaaca actaatgcgc caaacattta ttgcgcgtaa aatatcgttt atttcattaa
    16201 tacatttcag ggatgaatat atgtcaccgc agaataatca tctgcagcgt ccgcctgctg
    16261 ctgtgttata cgccgatgaa ctggcaaaat taaaacaaaa tgataacgca ccttgcccgc
    16321 ccggttggca gttaagtttg cctgcggccc gtgcttttat ccttggcgat agcgcgcaaa
    16381 atattagccg taaagtggtg attagtccct ccgctgtcga acgtatgtta gtcactcttg
    16441 ccaccgggcg cggtttgatg ttggtggggg aaccgggcac cgcaaaatct ctcctgtctg
    16501 aattactggc aaccgctatt agcggcgatg ccgggttaac catccagggc ggcgcatcta
    16561 ctactgaaga tcaaatggaa ctacgccctg cttatcaacc atggtccgtc tacggaagca
    16621 ctggtccctg ccccgcttta ccagggaatg cgcgacggca aaatcgtccg ttttgaagag
    16681 ataacgcgta caccgctgga agtacaagac tgtctgttag gtatgctttc tgacagggtg
    16741 atgacggtgc cggaactcac cggtgaagcc agtcagctct atgcgcggga aggctttaat
    16801 attattgcca ctgccaatac ccgcgatcgc ggcgtcaatg agatgagtgc cgcgctaaag
    16861 cgtcgcttcg attttgaaac cgtctttccg attatggatt tcgcccagga gctggaactg
    16921 gtcgccagtg cttcggcgcg tttgctggcc catagcggta ttccgcataa agtgcctgac
    16981 gcggtactgg agttactggt ccgcaccttc cgcgatctgc gtgccaacgg cgaaaagaaa
    17041 acgtcaatgg atactctgac ggcaattata tccaccgctg aagccgtgaa cgtggcccac
    17101 gcagtgggcg tccgcgcctg gtttttagcg aatcgcgcag gtgagccagc cgatctggtg
    17161 gactgtattg ccggaaccat cgttaaagat aacgaagaag atcgcgcgcg tctgcgccgt
    17221 tacttcgaac agcgtgtcgc cacacataaa gaagctcact ggcaggctta ttatcaagcc
    17281 cgccaccgcc tgccgtgagg aaagatgcat gagcgagccg ttaattgtcg gcatccggca
    17341 tcatagtccg gcctgcgccc ggctggtgaa atcgttaatc gaaagccagc ggccacgata
    17401 cgtgttgatt gaaggcccgg ctgattttaa tgaccgggta gacgaactgt ttttagccca
    17461 ccagcttccg gtagctattt acagttattg ccagtatcag gacggtgcag cccccgggcg
    17521 tggtgcctgg acgccatttg ctgaattttc gccggagtgg caggcgctac aagccgcacg
    17581 tcgtattcag gcacaaactt acttcatcga tttgccttgc tgggcgcaga gtgaagaaga
    17641 agacgaatca cccgatacgc aagatgacag ccagacctta ctgctgcgtg ccacccgcat
    17701 ggataacagc gacaccctgt gggatcactt gttcgaagat gaaagccagc aaactgcatt
    17761 accctctgcg ctggcgcact attttgcaca actgcggggc gattcccccg gagatgccct
    17821 caatcgtcag cgcgaagcct ttatggcccg ctggattgga tgggcgatgc agcaaaacaa
    17881 tggcgacgtg ttagtcgtct gcggcggctg gcatgctccg gcactggcaa agatgtggcg
    17941 cgaatacacg caggaaatta acacgccgga attgccctag ctggcagatg ccgttacagg
    18001 ttgttatctc accccctaca gtgaaaagcg ccttgatgtg ctggcaggat acctttctgg
    18061 tatgcctgcc ccggtctggc aaaactggtg ctggcagtgg ggcctacagc aggccggtga
    18121 acaactgcta aaaactatcc ttacccgttt gcgccagcac aaattgcccg cttctaccgc
    18181 tgatatggct gccgctcatc tgcatgcgat ggcgctggca cagttgcgcg gtcatacact
    18241 accgttacgc actgactggc tggatgccat agcaggctcg ctgattaaag aagccttgaa
    18301 cgcgccgttg ccgtggagct atcgcggcgt tattcatccc gataccgatc cgattctgct
    18361 aacgttgata gacacattag cgggtgacgg attcggtaaa cttgcccctt ctacgccaca
    18421 accgcctctg ccaaaagatg tcacctgcga actggaacgt accggaatct ctcttccggc
    18481 ggagcttacc ttaaatcgct ttacccccga tggactggcg caaagtcagg tgttacatcg
    18541 gttggcaata ctggagatcc caggggttgt gcgccagcag ggaagtacac tgtcacttgc
    18601 aggcaacggt gaagaatgct ggaaattaac ccgcccgctt agccagcatg cggcattgat
    18661 tgaggccgcc tgctttggtg ccacactcca ggaagccgca cgccataaat tagaagccga
    18721 tatgctggac gcgggtggaa tcagcatcat caccacatgt cttagccagg cggcgttagc
    18781 gggtctggcg tctttcagtc aacaattact ggagcaactt acattattaa tcgcccagga
    18841 aaatcaattt gccgaaatgg gccaggcgct ggaagtgcta tatgccttat ggcggttaga
    18901 tgaaatcagc ggtatgcaag gcgcgcagat attgcagacg acgttatgcg cggctatcga
    18961 tcgcacactc tggctgtgtg aatccaacgg caaaccggag gaaaaggagt ttcacgctca
    19021 cctgcatagc tggcaggcgc tttgccatat cctgcgcgat ctacatagcg gcgttaattt
    19081 acccggcgtt tcgctttctg cggcggtagc cttactggag cgccgcagtc aggcgattca
    19141 tgccccggcg ctggatcgcg gcgcggctct tggcgcacta atgcgtctgg aacatcccaa
    19201 cgccagtgcc gaagcggcgc tgacaatgct ggcgcaatta tccccggcac aatccggcga
    19261 agcgctgcac ggtttgctgg cgctggcccg ccatcaactg gcctgtcagc cggcatttat
    19321 cgccggtttc agtagtcatt taaatcaact gagtaacgac gattttatta acgccctgcc
    19381 cgatttacgc gcggcgatgg cctggctacc accacgagaa cgcgggacgc tggcgcatca
    19441 ggtgtttgag cattatcaac tggcgcaact tcccgtttcg gcactgcaaa tgccgttgca
    19501 ttgtccaccg caagccattg cacatcatca acaactcgaa cagcaggcac tggcatcgct
    19561 gcaacactgg ggagttttcc atgtctgaac tgaacgatct tctgaccacc cgtgagctac
    19621 aacgctggcg attaattctt ggcgaagcgg cagaaacgac cctttgtggg ctggatgaca
    19681 atgcccggca gatagaccac gcgctggagt ggctgtatgg gcgcgatcct gaacggctcc
    19741 agcgtggtga acgctccggt ggattaggtt gctcaaatct caccacccct gagtggatca
    19801 acagtattca cacgctgttt ccgcaacagg tgatcgagcg gctggaaagc gatgccgtgc
    19861 tgcgctatgg cattgaagat gtggtgacga atctcgacgt actggaacgt atgcagcctt
    19921 ctgaaagcct gctacgcgcc gttttgcaca ccaaacatct gatgaatccc gaagtgctgg
    19981 ctgccgctcg ccggatagtg cgccaggttg ttgaagagat tatggcgcga ctggcaaagg
    20041 aagttcgcca ggctttttct ggtgtccgcg atcgccgccg tcgctcattt attccactgg
    20101 cgcgtaattt cgatttcaaa agtacgctgc gtgccaacct gcaacactgg catccgcaac
    20161 acggcaagtt gtatatcgaa tccccccgct ttaacagccg cattaagcgc catagcgatc
    20221 aatggcaact ggtcttactg gttgatcaaa gcggatcgat ggttgactcg gtgatccact
    20281 cagcggtgat ggcggcctgt ttatggcagt tacccggtat tcgtacccat ctggtggcgt
    20341 ttgacaccag cgtggttgat ctcacggcag acgttgccga tccggtagag ttattaatga
    20401 aagtgcaatt aggcggcgga accaatatcg ccagtgccat ggaatatggt cggcaactta
    20461 ttgaacaacc agcgaaaagc gtcattatcc tcgtgagcga tttttacgaa gggggttcat
    20521 catcattact gacgcatcag gtgaaaaagt gtgtccagag cggcatcaaa gtgctgggac
    20581 tggcggcgct cgatagcacc gcaacacctt gctatgacca cgatacggcc caggcgctgg
    20641 ttaatgtcgg cgcacaaata gccgccatga caccgggcga gctggcatca tggcttgcgg
    20701 agaatcttca gtcatgaatt cactacgtcc ggaattatta gaactgacac cgcaggccct
    20761 gacagcgtta agcaatgccg gttttgttaa gcgcagtctt aaggaactgg aaaatggcaa
    20821 cgtcccggag atcagccatg agaacggcgc tttaatcgcc accttcagtg acggtgtccg
    20881 tacccagctg gcgaacggtc aggcactgaa agaggctcag tgcagctgcg gggccagcgg
    20941 catgtgccgc catcgcgtga tgctggtttt aagttatcag cgactttgcg ccaccaccca
    21001 gactacggaa aaagaagaag agtgggatcc ggcaatctgg ctggaagaac tggctaccct
    21061 gcccgatgcc acccgcaaac gcgcacaggc actggtcgct aaaggcatca ccattgagtt
    21121 gttctgtacg ccgagcgaaa ttccctctgc ccgcttaccg atgagcgatg tacgttttta
    21181 ttcccgcagc agcattcgtt tcgctcgctg tgattgtatt gaaggaacac tttgcgaaca
    21241 tgtcgtactg gcagtacagg cctttgtcca ggccaaagcg cagcaagcgg aatttactca
    21301 tttaatctgg cagatgcgca gcgaacacgt cacatcatct gacgatccgt ttgccaacga
    21361 tgagggggac gcgtgtcgtc aatatgttca gcaattaagc caggcgttat ggcttggcgg
    21421 catcagccag ccgcttattc attacgaggc tgctttcagc cgcgcgcagc aagcggcgga
    21481 acgctgcaac tggcgctggg tgagtgaatc gctacggcaa ctgcgcgcca gcgttgatgc
    21541 cttccacgcc cgcgccagcc actatcatgc cggagaatgc ttacgtcagc ttgcggcatt
    21601 aaacaatcga ttaaattgtg cacaagagat ggcccggcgc gacagtgttg gtgaagttcc
    21661 tcccatgccg tggcgcacgg tcgttggcgc tggcattgcc ggagaagcaa agcttgatca
    21721 tctgcggctg gtgtctttag gtatgcgttg ctggcaggat attgagcagt atggtttacg
    21781 catctggttt accgatcccg acaccggcag tattttgcat ctttcgcgta gttggccgag
    21841 aagcgaacag gaaaactcac cggcagcgac gcgtcggctg tttagttttc aggctggcgc
    21901 actggctggt gggcaaattg tttcacaagc agcaaaacgc agtgccgatg gcgagctgct
    21961 gttagctacc cgcaaccgct tgagtagtgt tgttccactg tcgccagacg cctggcaaat
    22021 gttgagtgcg ccgttgcgcc agccgggcat tgtggctttg cgtgaatatt tacgccagcg
    22081 tccccccgcc tgcatacggc ctcttaatca ggtggataac ttatttattc tgccagtcgc
    22141 tgagtgtatt tcactcggtt gggatagcag ccgccagacg ctggacgcgc aggtaatcag
    22201 cggcgaaggt gaagataatc tgctgacgtt atcccttccg gcatctgcct gttctccttt
    22261 tgccgttgaa cgcatggcgg cgcttttgca acaaacagac gaccccgtga gtctggtttc
    22321 tggctttgtc agctttgtag aggggcaatt gacactggaa ccacgggtaa tgatgaccaa
    22381 aacccgcgcc tgggcgctgg acgcagaaac tgcgcctgtg gcaccgctac cttctgccag
    22441 cgttttgcct gtgccgtcta ccgctcatca gttgctgatg cgctgccagg cgttacttat
    22501 tcaactgctc cataacggct ggcgctatca ggaacagagt gctattggtc aggcagagtt
    22561 gctggcgaat gacctcaccg cggtggtttt ttatcggctg gcacatgtgt tggcacaatt
    22621 tcgtaataca gaaagcgagg cacgggtaga agcaatgaat aacggtgttt tactttgcga
    22681 acaattattc cccatgcttc agcaacaagg atgaaatagt gctttttact aagagttcta
    22741 ctccagttcc ggactgctca cgtcacggta ttaggcatat cctatatagc tcctggtgag
    22801 agtcaccagt ttcttgatta aataaaatgg agttttacat gaaggctttc aataagctgt
    22861 tttccctcgt tgttgcatct gttctggttt tctctcttgc tggctgcggt gacaaagaag
    22921 aatcgaaaaa attcagcgcc aatctgaacg gtactgaaat tgccattacc tatgtctaca
    22981 aaggtgacaa ggtgcttaag caatcttctg aaaccaaaat tcaatttgcc tccattggtg
    23041 caaccaccaa agaagatgct gccaagacac ttgagccgtt aagcgccaaa tacaaaaaca
    23101 tcgcgggtgt tgaagaaaaa ttaacctata ccgataccta cgcgcaggaa aacgtgacta
    23161 tcgatatgga aaaagtggat ttcaaagccc tgcagggtat ttcaggaatc aacgtttctg
    23221 ctgaagatgc caaaaaaggt atcactatgg cgcaaatgga actggtgatg aaagccgctg
    23281 gttttaaaga agtgaaataa tctgtcggcg gccatgtttt tgcatggccg ccatacccgt
    23341 cttagctttt cttcacatgc tggcgcgctg ccagtccacg cagaaaataa cgtaaaaatt
    23401 gatcgccgca ttcgcggaag tttttatgat ccggtgcacg catcatcgcc gtaatttccg
    23461 gcatcgaaac gcggaactgc tgttcggtga ggatcgccag aatgtcatcg gtcttcagcg
    23521 aaaacgcgat gcgtaatttt ttcagcacga tgttgttatt aatgcgacgt tccggctcca
    23581 gtgccggagc agactcatcc ttgccgcgtt tttcataaat caggccattg aggaaggaag
    23641 acaaaacaat gtccggacaa cgctgaaaac cctcttcgtc ttctttacgt agccagacgg
    23701 cgatctgttc cgtggtggct tcgacattac ccagcgccag aatacgcacc aggtcattat
    23761 tattggcttt caaaatgtag cgcacgctgc gcagaatatc gttacttagc atgaggcctt
    23821 caggtgttga tgaggcaaaa agccatttta gcagtctttt acaggccaat cgcctctttt
    23881 aagcttttca gataacgacg gctgaccggc acggttaagc cattacgcaa aatcaactcg
    23941 gcctggccgt tatcttccag acgaatctcc tgtaaatgcg cgaggttaac cagatactga
    24001 cgatggcagc gtagtagtgg tgtacgactc tccagggtac gtaatgtcaa ttcggtaaag
    24061 ccctctttcc cttcgtgact ggtaacgtag acaccgctca tccgactgct gacaaatgct
    24121 acatctttca tttgcagcaa ataaatccga ctatgccccg tacaagggat aaatttcagc
    24181 gcctgttgat tttccggtaa cagcgaaaca tcctgcttgc tgcgctccag acgcaatcgc
    24241 gccagcgttt tctccagtcg cgcttcatca attggcttca gcagataatc aaaagcatgt
    24301 tcttcaaagg ctttaatggc gtattcatca aacgcggtga gaaaaacgat atacggacga
    24361 tgttccgggt cgagcatccc caccatttcc agaccactga tgcgcggcat ctggatatca
    24421 agaaacagca catccgggcg cagtttatgc accgcgccga tcccttccac ggcgtttgaa
    24481 cactctccaa cgatttcaat atcgctctgc tcctgcaaaa atacgcgcag gttctcccgt
    24541 gctaacggct catcatcgac aattaagact ttaatcatgc ctcgtccctc catggtagtc
    24601 gtaacgttat tcgggtgtaa ctatcaggct cacaggcgac gcttatccca tagtcatcgc
    24661 caaaccgttc acgtaaacgc ttatccacca gattcatccc cagcccactg gcattggtta
    24721 ccggttgata caaaccggcg ttgtcttcga tctccagcat caaatgttgc ccctcacgtc
    24781 gggcgctgat tgccactcgc cctgtatcca gcagttgtga tgtcccatgt ttaatagcgt
    24841 tttccactat cggttgcagg gtaaacgcgg gcaattgctg ctgggataat tcttgcggaa
    24901 tagcaatgtt gacctgcaac cgcgactgga agcgcgcctt ttcaatttgc agataagcgt
    24961 tcacatgttc aatttcgtcg gcgagagtaa caaactccga aggccgcttt aagtttttgc
    25021 ggaaaaaagt ggaaagatac tgcaccagct ggctggcctg ttcgctgtcg cggcggatca
    25081 ccgctttaat ggtgttaagc gcattaaaca aaaaatgggg gttcacctgg gcgtgaagca
    25141 gtttgatctc tgactgggtg agcatcgctt tttgccgctc atattgcccg gcgaggatct
    25201 gcgccgaaag cagttgcgca atcccctcac ccagcgtgcg attgattgaa ctgaataaac
    25261 ggtttttggc ttcatacaat ttgatggtac ccatcacccg ctgattttca ccacgcaacg
    25321 gaattaccag cgtcgacccc agtttgcatt gcggatgcaa agagcaacga taaggtactt
    25381 cgttgccatc agcgtagacc acttcaccgg tttcaatcgc ttttaaggtg taagtcgaag
    25441 aaatcggttt gccgggtaaa tggtgatcgt caccaattcc ggtaaaggcc agcaatttct
    25501 ctcgatcggt aatcgcgact gcaccaatat ccagctcctg atacagcacc tgtgccactt
    25561 tcatgctgtt cacttcgtta aacccctggc gcaaaatgcc ttccgtcgag gcagccactt
    25621 ttagcgcagt ggcagaaaaa gccgaagtgt atttttcaaa catcgcgcgt ttatcgagca
    25681 atatacgcat aaacagcgcc gcgccgacgg tattggtgac catcattggc gcagcaatat
    25741 tactcaccag acgcaccgca tcttcataag gtcgggcgat tgccaggatg atcaacattt
    25801 gcaccatttc agcgacgaac gtgacggcac cggcggtaat ggggttaaag actttatcag
    25861 tgcgcccgcg acggatcagg atgctgtgta ccaggccgcc gagcaaccct tcaacgatgg
    25921 tcgagatcat gcaacttagc gcggtcatgc cccccatcga atatcgatgt aacccgccgg
    25981 tcagaccaac cagcccaccg acgaccggac cgccgagtaa gccgcccatt accgcgccta
    26041 tcgcacgggt attggcaata gaatcgtcaa tgtgcaaccc aaaccaggtg cccatgatgc
    26101 agaagatgga aaagacgatg tagcagagaa atttatgcgg cagacgaacc gtgacctgca
    26161 ttaacggtat gaataatggc gttttactca ttaaccacgc aatgactaaa aaaacgcaca
    26221 tctgctgaag cagcagcaac accagattaa aatcgtacat acccgcaaac cacacttccc
    26281 tttaaaacgc gtaacataca ttgcctgcgt ttaactttct ttgaactctt gcagaaaaat
    26341 gagaattcgt gagtacgatc actcaaaatc gcctggcaaa aataaaatca ccctatagat
    26401 gcacaaaaaa cgggcaaaac tacctggttc gcaaaactgc gtctaaagtt aaaccgggac
    26461 ctcgcgagca agggtgagac gatggcgctt tacacaattg gtgaagtggc gttgctttgt
    26521 gatattaacc ctgtcacgtt acgcgcgtgg cagaggcgtt acggattgct gaaaccgcaa
    26581 cggacagacg gcggccatcg gctgttcaac gatgccgata ttgaccggat ccgcgagatc
    26641 aaacgctgga tcgacaacgg cgtgcaggtc agcaaagtta aaatgctgct cagtaatgaa
    26701 aatgttgatg tgcagaacgg ctggcgcgat cagcaagaaa cattactgac ttacctgcaa
    26761 agcggcaatc tgcatagcct gcgaacgtgg atcaaagagc gcggtcagga ttaccccgcc
    26821 cagacgctca ccacacatct gtttattcct ctgcgccgac gacttcagtg ccaacaaccg
    26881 actctccagg cgctgctggc gatcctcgac ggcgtactga tcaactacat cgccatttgc
    26941 ctggcttcgg cacgtaaaaa acagggtaaa gatgcgttgg tggttggctg gaatattcat
    27001 gataccaccc gtctgtggct ggagggctgg attgccagtc aacaaggatg gcgcattgat
    27061 gtcctcgccc actcgctcaa tcaactacgc cctgaactgt tcgaaggccg tacattgctg
    27121 gtgtggtgcg gtgaaaatcg aacctccgcc caacagcagc aactcaccag ttggcaggaa
    27181 caaggccatg atattttccc actcggcatt taatgattcg ttaacaaatg cgctttactg
    27241 tacaatcctt tcgttaacat aaggagtgca ttatgcgcat agctaaaatt ggggtcatcg
    27301 ccctgttcct gtttatggcg ttaggcggaa ttggtggcgt catgctcgca ggttatacct
    27361 ttattttgcg tgctggctaa gcgcctgcac cagcctttca aacaggcggt ctgcaatgat
    27421 cgccgccagt gccaccagta acgccccctg gatcacatac gcggtattaa atccgctaag
    27481 cccgatgatg atcggcgtac ccagcgtgct ggcccctacc gttgaggcga tcgtcgccgt
    27541 accaatgttg ataatcaccg aagttcgcac gcccgccaga atcaccggag ccgccagcgg
    27601 tagctcgacc ttacgcagtc gctgaccacg gctcattccc atacctttcg caacttctgt
    27661 cacgctggca tcaatcgctc ccagcccggc aagtgttgcc tgcaggacgg gcagcacacc
    27721 ataaaggatc aaggcgataa tcgcgggttg cagaccaaac ccaatcaccg gaacggcaat
    27781 cgccagcacc gcgacgggcg gaaaagtctg tccaacggcg gcaatagttt ccaccagtgg
    27841 gcgaaattcc gcgccccacg ggcgagtaac aataattcct gcaccagtgc caatgatcac
    27901 cgcaaacaaa ctcgaaattc ccaccagcca gaaatgagcc agtgccagag cagcaaaact
    27961 ttcttgctga taaacgggtc gtggcagttg tgggaacaag gcagcaaaca gcggctggct
    28021 gtaaggcagc cagaaaatca gcgccacaaa cagagcaatg agccagaaca gcggatcgcg
    28081 caacatcttc atacgtttac gcctccacca gcagatcctg aaaatgcagc gtgccgcaag
    28141 gctggccctg cgtgttcacc accggcagca cctcgcatcc ccgcgcgaca aacagggaga
    28201 gcgcatcgcg tagagtcatc tcttctgcca gcgcctcacc ttctgcccgt tcttcgcgac
    28261 gcacgtaatc cgccacgcta cgtaacgaaa gcaggcggac acccagttca ctatgcccaa
    28321 aaaactggcg gacaaaatca ttcgccggac gagtcagcat cgtcagcgga ttgccctgct
    28381 gcaccacttc accgtgatcc atcaacacca gatgttctgc cagtcgtagc gcctcatcaa
    28441 tatcatgagt gaccagcaca atggtacgcc ccagcaaacg gtgtatgcgc gtcatctctt
    28501 gttgcaacgc gccgcgcgtt accgggtcca gcgcgccaaa aggttcatcc atcagtaaga
    28561 cttgcggatc ggcagccagt gcgcgcgcca ctcccacacg ttgctgctga ccgccagaaa
    28621 gctgatgcgg ataacgctca cgcaaatttg gctccagccc cagtagcgcc attaattcgt
    28681 cgatacgatc gtcaatccgc gcccgcgacc atttttgtaa ttgcggcacg gtggcaatat
    28741 tttgcgccac gctccagtgg ggaaacaggc caatagattg aatggcatag cccatccggc
    28801 ggcgcaactc cagcactggc agcgagcgaa tttcttctcc ggcaaagcgg atcacgccgc
    28861 tgtcatgctc caccaggcgg ttaatcattt tcagggtggt ggatttaccg gagcctgatg
    28921 taccaatcag caccgaaaaa ctcccctcct gaaaattgag attgagatcg ttaacggctt
    28981 tttgtgcgcc gaacagtttg ctgacatggc taaattcaat cattacgttt caccttcagc
    29041 agtgcgataa gcaaatcgaa cagcgcatcg atcagcaccg ccagaacaat taccgggatc
    29101 acccccagca acactaaatc aatggcactg cttagcagcc cctggaaaac cagcgcacca
    29161 aaaccgcctg cgccgattaa cgccgcaatc accgccatgc cgacggtttg caccatcacc
    29221 acccgcaggc tacgcagaaa taccggtaac gccagcggta actgaacatg caggaatcgc
    29281 tgcgccccgc tcatgcccat cgctctggcg ctctccagca catcgcgcgg gatctggttc
    29341 aagccgacta ccacgccgcg caccagcggc agcaaggcat agagcaccag cgcaatcagt
    29401 gcgggtgtca ttccggttcc tgctatgccg agcttaccca gccacggaaa ggccgtaacc
    29461 agtccggcaa gcggcgcaat caacaagcca aagagcgcca ccgaaggcac ggtctgaatg
    29521 acattgagta gcgaaaaaat cgccccctgc cgcgcagtgg aaaagtagca ccagacgccc
    29581 aacggcacac caatcactaa cgcaggcagc accgcaccaa acagcaacgt cagatgttgt
    29641 gccagcgcgt cgtcaaacac atcctgacgg ttggcgtatt ctttcattag cgagagatcg
    29701 ttaagcgtgc cggagtacag caaccacagc ggaataatgg caatctgcat atgcaacaac
    29761 cagcgccaca gcggatgcgt ggagattcgg cggatggcat cgctacaggc cagcaatgcc
    29821 agcgccgcag ccagccagaa accactgccg aggctggtac gcgccagcgc actgccattt
    29881 tgtgccagtt gtgtcgccgc ctttcctgct ccccacacca gcaatacgaa gacgaattgc
    29941 gccagaatga gtgcaaaaat gctgcctttt ttagcgggaa taaaacaggc tgtcatccag
    30001 gcgcaaccaa cgcccaccag catccagatc gtttgcggcc acagctgcca gagatgacgc
    30061 ccctcacccg aaactaaacg attaggcgcg taactgataa atggcagcgc cgctgcgatt
    30121 gccgtcagca acagcagcag cgccagaaca ggattaatac ggaaataagt cacgggaaat
    30181 tacttcgtcc accctttttg tttcaggtag tcggcagcca cttttttggc atccagtcct
    30241 tccacagcaa tgctggcatt cagttgctgc aatgtttttt catcgaggct ggcgaagact
    30301 ggctgtagcc actgtgccat ttgtggatac tctttcaaca ccgactcacg caccactggt
    30361 gcaggcgcgt agataggttg cacgccttgc ggatcactta aggtttgcag ccccagcgcc
    30421 gcgaccgggc cgtcagtgcc gtaagccatc gccgcattaa cgccagaggt ttgctgggca
    30481 gcggctttga tcgtcaccgc cgtgtcgcca ccagccagtg acagcaactg atcctgaccg
    30541 agcttaaagc cataggcttt ttcaaacgcg ggtaacgcat cggcgcgttc aataaattct
    30601 gccgaggccg ccagtttgaa ggtgccgccc tctttcagat aacgactcag gtcggcaagc
    30661 gaagtgagtt tgtttttctc tgccacatcc tgacgcacgg cgatagtcca ggtgttattt
    30721 gcaggcgcgg gcgtcagcca gattaacttg ttttgctctg catcgagttt tttgactttc
    30781 tcgtagcctt gcccggcatt tttccatgcc gcatcgtttt catctttaaa gaaaaacgcg
    30841 ccattgccgg tatattccgg atagatatcc agttcaccgg aagtaatcgc cccgcgcacc
    30901 acgggagtcg ttcccagttg cactttattt accgtaggaa caccgtgact ttccagcacc
    30961 tgcaaaatga tattgccgag cagcgcgcct tcggtatcga tttttgaacc gaccttaacg
    31021 ggggaagccg cctgtagcgg caggctcacg gctgccaaca aaaccaatga acctgcccag
    31081 agctttgaga gtggcatgat gctttcctca ttcttttact gttgttttca gcgaattaag
    31141 agaaaagcat agttgataat gacagggtta gcctgtctgg gcggattcag ttgcagaatc
    31201 agataaatac ttaaagggcg cagacgtcgc gcccttacta aagcattaca gcaactcaaa
    31261 ctcgcctttc ttaacgcgtg cggaatcagt gccgataaag acattgaact tgccaggctc
    31321 ggcgtcatat ttcatctgtt gattccagaa cttcagcgcc tcgatatcaa tcgggaagct
    31381 gacggtctgg gtttcgcccg gtttcagggt gattttctca aagcctttca gctgcttcac
    31441 cggacgactc atggaagccg tcacatcctg caagtacatc tgcactaccg tcgccccttc
    31501 gcgcttaccg gtgttcgtca cctgcacact ggcggtcact ttgccgtcac gcttcatggt
    31561 cggcgcagaa agtttcacat cagagacggt gaaagtggta tagctcagac catagccgaa
    31621 cggataaagc gccccgttag cttcatcaaa ataacgcgaa gtgtatttgt tcggcttatc
    31681 ggcgttatac gggcgaccgg tattcagatg gctgtagtac acagggatct gcccgacaga
    31741 acgcgggaag gacatcggca gcttgccgga cgggttgtaa tcgccaaaca atacatcggc
    31801 aatcgcattc cccccttcag tccccgcaaa ccaggtttcc agaatcgcat cagcctgctg
    31861 atcttctttc accagcgcca gcggacgccc attcatcagc accagcacca gcggtttacc
    31921 ggtggctttc agcgccgcaa tcaagtcacg ttggctttgc ggaatagtga tatcggtacg
    31981 gctggaagcc tcgtgcgcca tcccctgtgc ttcaccgacc acagccacca ccacatcaga
    32041 ttgtttcgcg gtttgcaccg cttcatcaat catctcttgc ggcgaacgcg gatcgacttt
    32101 gaccgcttct tcatactgat tcaggaaatc gataatgcct ttgtcactgg taacgttagc
    32161 ccctttggca tacagcactt taccgttttc accgacggca tttttaatcc cggtcagcac
    32221 ggtcacggat tgatcggcaa cccctgccgc cgaccagctg cccatcacgt cacgcttgct
    32281 gtctgccagc gggccaacca ccgcaatggt tgcggatttt ttcagcggta acgtttcgag
    32341 acggtttttc agcaacacca ggctttcgcg cgccacttca cgcgcttctt tgcggtgcag
    32401 gcggctttcg gcattggtat ccaccgggtc agactctttc ggaccgagat ggctgtacgg
    32461 gtcgttaaac aaccccatat catatttaac gttcagtaca tgacgggtag cgtcatccag
    32521 ctcttccatc gtcactttgc cggatttgat caacccaggc agatacttcg agtagtactc
    32581 gtcgctcata ctcatgttga tgccggattt cagcgccacg cgcaccgcat cttccgggtc
    32641 tgccgccgtg ccatgtttaa tcagctcttt gattgcgccg tgatcggaaa cggtgatgcc
    32701 tttaaagccc cactggtcgc gcagaacatc tttcagcagc caggagtcgg aggtggctgg
    32761 cgtaccgttc agcgagttca gcgccaccat caccgcgccg ctgcctgcct ccagccccgc
    32821 tttgtacggc ggcatataat cattaaacag gcgctgcgga ctcatatcga cggtgttgta
    32881 ctctttaccg ccttctaccg cgccgtaagc ggcaaagtgt ttgacgctgg tcatcaccga
    32941 gtagcgatct gccggacttt taccctgcat cgcttccacc atggttttcc ccattattga
    33001 ggtgagatac gtatcttcgc caaaaccttc ggaagcacgt ccccagcgcg gatcgcgcga
    33061 gacatcgacc attggtgccc aggtcatatt caggccatca tctgccgctt cataagcaga
    33121 gacacgcccg actgttttca ccgcatcgag gttaaaagac gaggccagac cgaggctaat
    33181 cgggaacacc gtgcgctgac cgtgcagcac gtcgtaagca aagaaaagag gaattttcag
    33241 gcggcttaat tccatcacct gatcctgcat ggcgcggata tcctgacggg ttacggtgtt
    33301 gaaaatcgcc ccaacctgcc cgtctttgat catctcgcgg atcgcctctt tcgggttatc
    33361 cgggccgacg ctgattaagc gcagctgacc aattttctca tcaactgtca ttttcttaag
    33421 cagttcggtg acgaacgcat cccgcgcttc aggcgttaat ggatggttac cgaataaatc
    33481 atccgccagt gctggctgca gagccagact caccgcgatt cctactgaac atagccattt
    33541 catatggatt tttcttcctt tgttgcccga cgtggcagcg aaaatggtgc aaaaaccgta
    33601 gtttgccata agcatgatgg agagagaaaa agaatgctca gtttattgtc tgaattttca
    33661 aaatattcac tcgctgaatt gtcatacaag gcgctattct agtttgtgat attttttcgc
    33721 caccacaagg agtggaaaat gtcttccatg acaacaactg ataataaagc ctttttgaat
    33781 gaacttgccc gactggtggg ttcttcacac ctgctcaccg atcccgcaaa aacggcccgc
    33841 tatcgcaagg gcttccgttc tggtcagggc gatgcactgg ctgtcgtttt ccctggctca
    33901 ctactggaat tgtggcgggt gctgaaagcc tgcgtcaccg ccgacaaaat tattctgatg
    33961 caggcagcca atacaggtct gaccgaaggc tcgacgccaa acggtaacga ctatgatcgc
    34021 gatatcgtga tcatcagcac cctgcgtctc gacaagctgc acgttcttgg caagggcgaa
    34081 caggtgctgg cctatccggg caccacgctc tattcgctgg aaaaagccct caaaccgctg
    34141 ggacgcgaac cgcactcggt gattggatca tcgtgtatag gcgcatcggt catcggcggt
    34201 atctgtaata actctggtgg ttcgctggtg caacgtggcc cggcgtatac cgaaatgtcg
    34261 ttattcgcgc gtataaatga agatggcaag ctgacgctgg ttaaccatct ggggattgat
    34321 ctgggcgaaa cgccggagca gatccttagc aagctggatg acgatcgcat caaagatgac
    34381 gatgtgcgtc acgatggtcg tcacgcccac gattatgact atgtccaccg cgttcgtgat
    34441 attgaagccg acacgcctgc acgttataac gccgatccgg atcggttatt tgaatcttct
    34501 ggttgcgccg ggaagctggc cgtctttgcg gtacgtcttg ataccttcga agcggaaaaa
    34561 aatcagcagg tgttttatat cggcaccaac cagccggaag tactgaccga aatccgccgt
    34621 catattctgg ctaatttcga aaatctgccg gttgccgggg aatatatgca ccgggatatc
    34681 tacgatattg cggaaaaata cggcaaagac accttcctga tgattgataa gttaggcacc
    34741 gacaagatgc cgttcttctt taatctcaag gggcgcaccg atgcgatgct ggagaaagtg
    34801 aaattcttcc gtccgcattt taccgaccgt gcgatgcaaa aattcggtca cctgttcccc
    34861 agccatttac cgccgcgcat gaaaaactgg cgcgataaat acgagcatca tctgctgtta
    34921 aaaatggcgg gcgatggcgt cggtgaagcc aaatcgtggc tggtggatta tttcaaacag
    34981 gccgaaggcg atttctttgt ctgtacgccg gaggaaggta gcaaagcgtt tttacaccgt
    35041 ttcgccgctg cgggcgcagc aattcgttat caggcggtgc attccgatga agtcgaagac
    35101 attctggcgc tggatatcgc tctgcggcgt aacgacaccg agtggtatga gcatttaccg
    35161 ccggagatcg acagccagct ggtgcacaag ctctattacg gccattttat gtgctatgtc
    35221 ttccatcagg attacatcgt taaaaaaggc gtggatgtgc atgcgttgaa agaacagatg
    35281 ctggagctgc tacagcaacg cggcgcgcaa taccctgccg aacataacgt cggtcatttg
    35341 tataaagcac cggagacgtt gcagaagttt tatcgcgaga acgatccgac caacagtatg
    35401 aatccgggga tcggtaaaac cagtaagcgg aaaaactggc aggaagtgga gtaaaaatta
    35461 cggatggcag agtatcgcca tccgtaattc acttaatcgt tctgtgccgt ctgccccgcc
    35521 gccgccattt gggcggcttt ttgtttttta tagctcaacg ctgctgccgg aacaggcatc
    35581 actttaccgg tttcaatcca ggtacgcagg cggctggcat cggcaaaatg ggtatatttg
    35641 ccaaacgcat ccatcactac cagcgccacc ggtttattat tgataaccgt acgcatcacc
    35701 agacaatggc ccgccgcatt ggtaaagccg gttttggtta gctggatatt ccagttatcg
    35761 cgatacacca gatggttagt attgcggaac ggcagcgtat acgtcgggtt agagaaggtt
    35821 gccatatctt cccgggtagt acttaactgc ccgatcaacg gatattgttt gctggcaatg
    35881 agcagtttgg ttaagtcacg ggcagttgaa acgttatgca ccgatagtcc ggtcggttca
    35941 acaaagcgcg tgttgttcat tccgagcgat ttcgctttcg cattcatcgc cttaataaag
    36001 gctttgtaac caccgggata atggtgcgca aggcttgccg ccgcgcggtt ttctgaagac
    36061 atcagcgcca gcaacagcat atctttacgg ctgatttcgc tgttcagtcg tacgcgcgaa
    36121 tagacccctt tcatctccgg cgtctggctg atatccactt ttagtttttc atccagcggc
    36181 agtcgtgcat ccagcacgac catcgcggtc attaatttgc tgatagacgc aatcggacgc
    36241 accagatccg ggtggttcga atagatcact ttgttggtat tcagatcaac aatcatcgcg
    36301 ctaccggagg caatttccgg ttgtgaagcg gtggtagcgg ctgccgtttt cgcaacggcc
    36361 tgcggtgcaa aaggcacagc cagcatcagg gccaggctaa ataaagaaac tcgaaatttc
    36421 ggcatgatga gcattcatat agtggttcac gcgcacgggt tgcgcaccgc cggagtaagg
    36481 atttactgag gctagcgacg ccatcataac gagcaaaaag tgcgatcgtc aaaggagaat
    36541 cgtgaggaaa tgctgcattg ctgacattta cgccagcaat gcaacacaaa agaactttct
    36601 agaacaaacg ataaccgtag ccccatagta taacggtaag ggcgagcagc acttccagta
    36661 ccagcacgcc aatcgccagc gtcgaactgg agaagctaag tccatcctct ttgttgatat
    36721 tcaggaagct cggaataccg aggtaaagca ggtagccggt gtaaaacagc gccaccgtgc
    36781 cgaccagcgc acacaaccag accagcggat aaagcgccac cagaccgctt aaaaacagcg
    36841 gagttgcaac gtagcccgca aagaccatgc agtgcgcaag tgacggacgc tgcggataat
    36901 tgcgcgccat ccaccagatg acccgcccca tcaccgcgac cccagccagc ataacgccat
    36961 aaaacaagac agccagcgcc agtccggtaa accaggataa cttcaggata gtgccatcgc
    37021 caaaattcca gccaatttgt gtggtgccaa tgaaggcgca aatcaccgga atcgccgcca
    37081 tcagcaaaac gtggtgggtg tagtgatgag aaatcgtttc gttttcgcga ttaatcacct
    37141 gcatttcacg atcgggatgg gaaaacagtc cccagacatg gctcataccg cccccttgtt
    37201 gtgagttcat gaacctgaca gttcaagtat aagtcagctt gtgattattt tttgttcgct
    37261 catgtaattt gcaccgtctg atgtacgtcc tttttcagcg tgaactattc acagggtgta
    37321 tgcttacagg caagcaaagg gagacagact ggcctatgga tctcaataca ctcatctcac
    37381 aatatggtta tgccgcgctg gtgatcggta gcctggcgga aggtgaaacc gtgactttgc
    37441 tgggaggcgt tgcggcacat caggggctat taaagttccc gctggtggta ctttctgtgg
    37501 cgcttggcgg catgattggc gaccaggtgc tttatctgtg cgggcggcgc tttggcggca
    37561 agctattacg ccgtttctcg aaacatcagg ataaaattga gcgggcgcag aaacttatcc
    37621 aacgccatcc gtatctgttt gtcattggta cgcgttttat gtatggcttt agggtgattg
    37681 gcccgacgct gattggtgcc agccagctgc cgccgaaaat ctttctgcca ctgaatatcc
    37741 ttggcgcatt tgcctgggcg ttgattttta ccactatcgg ttacgctggt ggtcaggtga
    37801 ttgcgccgtg gttgcacaat ctcgaccagc atttgaagca ctgggtctgg ttgattctgg
    37861 tcgtggttct ggtggtaggc gtgcgctggt ggctgaaacg acgcgggaag aaaaagccgg
    37921 ataatcaggc gtaaagcagt tgccacagat aagcattcac gccacatccg gcaccgggtg
    37981 ttccatgcct gatgcgacac tggcgtgtct tatctggcct aacccaatga aatatccacg
    38041 cacctcactg cgctattctg ggttgaactg cggattcgcc agcataaagc cgccatccac
    38101 tatcaacgac tgcccagtgg tgtaatttgc gccctccgaa caaagccaca ccaccaggct
    38161 ggcaatctca tgcgttgcgc caaaacgccg caagggaatc gaaggctccg cgccgggctt
    38221 cacgtcgcca tcatccatgc cgttcattgg cgtggcgatc gccccaggtg caaccgcatt
    38281 caccaaaatc ttatgcctga ccagctccag cgccatcgct ttggttaacc cgccgagcgc
    38341 atgtttagcg gctgtgtagg cgctggcatc cggcaacggc gtatgttcat gcaccgacgt
    38401 aatgttgatg atgcgaccac cctgcccttg tttcaccatc tggcgggcag caatttgcga
    38461 gcataaaaat gcaccatcga catcaacggt aaaaatcttg cgccactcat cgaaagccat
    38521 atcaagaaac ggcgctttgg tcattgcgcc cgcattattc accagcacat caatacgccc
    38581 cagccgttga atgagtttct ccagcgcctg cacaccttct ggcagattgc cgagatccag
    38641 ttgcacgatc tccgcacgta cgccgtggct aaccacctca cgcgcggtat cttttgcccc
    38701 ttcttcatca gagtgccagg taataccaat atcaaccccc tgctgcgcca gtaataacgc
    38761 gcactctttg ccgatccccg aatcggaggc ggtaataatc gcgacctgtg ccatcgagtt
    38821 ctccacttaa cgctgaataa acgttaagta tagaaggcgc atatcatcaa cgtttgtaac
    38881 ccccgcccaa cgcaccagtg agttgaatgg aagcatccag ccactgccct tgcaataaca
    38941 ggccattggc ccgctcacgc agcgccggga ttctggcttc gctgacccgg gaacccgcaa
    39001 tgatgcccgc gttaaagcgc gcctgcgcaa gacctactac acgcaaggca tcgcgctcaa
    39061 tttgcgcctg atgctggttt ttctccgcca gtgtctgaac ctgactagct gcccgcgcca
    39121 cgtcattcac cgcttcaacc accgctttgt tgtagctggc gatagacaag ttgctttcgg
    39181 cttttgcgat atcgaggttg gcgttaagac ggccactatc gaaaatgggt agcgtcaggc
    39241 ctgccgtaac gcccatttgc tgcgcggaat gacggaacag atcgcttaag tgcaacgcat
    39301 cctgttgcag gaaggccatc aggttgatgt caggataaaa tgccgctttt gccgcatcaa
    39361 tagtgcttaa cgatgactca acgtaccagt gcgccgcctg caaatctgcc cgccgggcca
    39421 gtaaggagta ccccagttca tcaggaagct ggcttgccac tttcggcaac gcgaccggat
    39481 taagcttcaa tgcctttgtc tggtgatttg taagtgcgct taaccgtgcc tcaataattt
    39541 tcattttccc cgcgacatcg ttgagctgct gccgggtttt gctggcatta atatcggttt
    39601 ccacaccttc aactgaagaa gtaatcccgt tctgatatag ctggcgatcg gtggcgataa
    39661 tggtgttctg ctctttttcg atttgctgta agacagtgtt taacgccgcc tgggtttgcc
    39721 actcccagta cagacgggct acgctgccag ccagcaattg gcgggtttgc tcgcgttccg
    39781 ccgcccgtgc tttaaccgta cccaggcgag cagtaacctc cgcccgattc tttccccaga
    39841 tatcgagatg ccagcccgcc gttaagccaa aagtaccgtt ggtgtaccac ggaccggtcg
    39901 tacctgcggc cggatcgttc aaagcaaacg gccccattaa cccttctgcc gacatttttt
    39961 gccgctccat atccgcagaa aagtcgagct gcggaccatc ctgagtggca actgccttcg
    40021 cctgggcttc agccagttga atgcgttgtt cagccacctg catatccggt gcgttctgta
    40081 gtgcgttatt aattaatgaa gtgagttgat tatcgtgata ctccagccac cattggctgt
    40141 ctggccaacc atttttcagc gccgtgggta atgcggtgtc aacttgcaca gcgggcgttt
    40201 gctggcttaa cgcctggcgg gtttcatgca taggcgcaca cccggccagc atcagtaaca
    40261 gcggaaaaca ggcgatggct ggataaaaag aatcacgatt catgggggaa taatcaggta
    40321 agaaaaggtg cgcggagatt accgtgtgtt gcgatatatt ttttagtttc gcgtggcaat
    40381 acatcagtgg caataaaaca acatattcag aaaaatatac actaagtgaa tgatatcttc
    40441 cgatttatct taatcgttta tggatagcgg caaagggctt cgttttttcc tatacttatt
    40501 cagcactcac aaataaagga acgccaatga aaattatact ctgggctgta ttgattattt
    40561 tcctgat
//
LOCUS       DACBVI010000023        39662 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.023, whole genome shotgun sequence.
ACCESSION   DACBVI010000023 DACBVI010000000
VERSION     DACBVI010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.023"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..665)
                     /locus_tag="IU325_06165"
     CDS             complement(<1..665)
                     /locus_tag="IU325_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808625.1"
                     /translation="MSNPCPSTFLDKNRIIAAELNITNNEKKYSPGSNFAKWMLQEIK
                     RLIANIMSGSRSVNTEILNYFHPMPGTENNGNRTWMAATGEDEYIEIKQTGDKSFNIT
                     LVGRDKRLRKETPYSGVAVATIIKSLSEKTAALETHSADTVLRKKLVNSIVINNTDFN
                     YEIPAGILSNIYDLLSLRVQKDEGYVPVQESFKRTDVFFDSIIMDAHRTIKEQNQQNG
                     ATK"
     gene            complement(960..1250)
                     /gene="ilvN"
                     /locus_tag="IU325_06170"
     CDS             complement(960..1250)
                     /gene="ilvN"
                     /locus_tag="IU325_06170"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HAP5808626.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(1254..2942)
                     /gene="ilvB"
                     /locus_tag="IU325_06175"
     CDS             complement(1254..2942)
                     /gene="ilvB"
                     /locus_tag="IU325_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HAP5808627.1"
                     /translation="MASSGTTSTHKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIHDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(3048..3146)
                     /gene="ivbL"
                     /locus_tag="IU325_06180"
     CDS             complement(3048..3146)
                     /gene="ivbL"
                     /locus_tag="IU325_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HAP5808628.1"
                     /translation="MTTSMLNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            3711..3800
                     /gene="tisB"
                     /locus_tag="IU325_06185"
     CDS             3711..3800
                     /gene="tisB"
                     /locus_tag="IU325_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HAP5808629.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            4080..5264
                     /gene="emrD"
                     /locus_tag="IU325_06190"
     CDS             4080..5264
                     /gene="emrD"
                     /locus_tag="IU325_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HAP5808630.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(5272..5769)
                     /locus_tag="IU325_06195"
     CDS             complement(5272..5769)
                     /locus_tag="IU325_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP5808631.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     HSRRGCQACDWHRLCPHAREETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(5766..6128)
                     /locus_tag="IU325_06200"
     CDS             complement(5766..6128)
                     /locus_tag="IU325_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HAP5808632.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(6118..6465)
                     /locus_tag="IU325_06205"
     CDS             complement(6118..6465)
                     /locus_tag="IU325_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HAP5808633.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMI
                     VAVIVMGLVLYAG"
     gene            6573..7022
                     /locus_tag="IU325_06210"
     CDS             6573..7022
                     /locus_tag="IU325_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808634.1"
                     /translation="MGIIAQNKISSLGMLFGAIALIMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCGLLLMFLIFSFLTGGSLA"
     gene            complement(7069..8562)
                     /locus_tag="IU325_06215"
     CDS             complement(7069..8562)
                     /locus_tag="IU325_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP5808635.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAESIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALANIEKPEILPGENILAAKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDLNEMHNLIDDIRFADVRRKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(8559..10274)
                     /locus_tag="IU325_06220"
     CDS             complement(8559..10274)
                     /locus_tag="IU325_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HAP5808636.1"
                     /translation="MNSLQILSFVGFTLLVAIITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPIFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFSVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            10441..11334
                     /locus_tag="IU325_06225"
     CDS             10441..11334
                     /locus_tag="IU325_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP5808637.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYSDGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(11331..12653)
                     /locus_tag="IU325_06230"
     CDS             complement(11331..12653)
                     /locus_tag="IU325_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HAP5808638.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGAR
                     QEVIAEACKVILKERAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMPQ
                     LRQYVSKHGYVPPQQDTHTEASWNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSAAVAKAILDDLLEANKAYWPELR"
     gene            complement(12653..14269)
                     /locus_tag="IU325_06235"
     CDS             complement(12653..14269)
                     /locus_tag="IU325_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP5808639.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            14565..15281
                     /locus_tag="IU325_06240"
     CDS             14565..15281
                     /locus_tag="IU325_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HAP5808640.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNANEYQVEYHLRRLHPEKS"
     gene            complement(15278..16939)
                     /locus_tag="IU325_06245"
     CDS             complement(15278..16939)
                     /locus_tag="IU325_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HAP5808641.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIRTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(17135..17563)
                     /gene="ibpB"
                     /locus_tag="IU325_06250"
     CDS             complement(17135..17563)
                     /gene="ibpB"
                     /locus_tag="IU325_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HAP5808642.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(17675..18088)
                     /gene="ibpA"
                     /locus_tag="IU325_06255"
     CDS             complement(17675..18088)
                     /gene="ibpA"
                     /locus_tag="IU325_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HAP5808643.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(18082..18154)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            18394..18726
                     /locus_tag="IU325_06260"
     CDS             18394..18726
                     /locus_tag="IU325_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HAP5808644.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(18728..19978)
                     /locus_tag="IU325_06265"
     CDS             complement(18728..19978)
                     /locus_tag="IU325_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HAP5808645.1"
                     /translation="MMAGTVLYQDRAMKQITFAPRNHLLTNTNTWTPDSQWLVFDVRP
                     SGASFTGETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWH
                     YDFHHRRGVIVEGGKMSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVM
                     HQLCPALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAY
                     EEGWVGNHALAFIGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPP
                     RGVVQRRLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGE
                     PRQLTHNKTDIQSAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAV
                     VFSPDGQWLAWMEGGQLWITETDR"
     gene            20043..21107
                     /gene="cbrA"
                     /locus_tag="IU325_06270"
     CDS             20043..21107
                     /gene="cbrA"
                     /locus_tag="IU325_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HAP5808646.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASFTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMNAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKSNAAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(21104..22441)
                     /locus_tag="IU325_06275"
     CDS             complement(21104..22441)
                     /locus_tag="IU325_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5808647.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISNA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(22516..23664)
                     /gene="dgoD"
                     /locus_tag="IU325_06280"
     CDS             complement(22516..23664)
                     /gene="dgoD"
                     /locus_tag="IU325_06280"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAP5808648.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELSDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNTPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(23661..24278)
                     /locus_tag="IU325_06285"
     CDS             complement(23661..24278)
                     /locus_tag="IU325_06285"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HAP5808649.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDVLARMGCQLIVTPNIHSEVIRRVVGYGM
                     TVCPGCATATEAFTALDAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSIERTAQQAAAFVKAYREAVQ"
     gene            complement(24262..25140)
                     /locus_tag="IU325_06290"
     CDS             complement(24262..25140)
                     /locus_tag="IU325_06290"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="catalyzes the formation of
                     2-dehydro-3-deoxy-D-galactonate 6-phosphate from
                     2-dehydro-3-deoxy-D-galactonate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-3-deoxygalactonate kinase"
                     /protein_id="HAP5808650.1"
                     /translation="MTARYIAIDWGSTNLRAWLYHGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQIFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(25137..25826)
                     /gene="dgoR"
                     /locus_tag="IU325_06295"
     CDS             complement(25137..25826)
                     /gene="dgoR"
                     /locus_tag="IU325_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HAP5808651.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            26104..26760
                     /locus_tag="IU325_06300"
     CDS             26104..26760
                     /locus_tag="IU325_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808652.1"
                     /translation="MKLNFKGFFKAAGLCSLAFALTGCITWGLVSNTASRPHAEQWRS
                     DTIKGLSLAEDSNGTKGYVFVGESLDYLLTTGGDKVVKMLNDPAIHGERITVSDNAKF
                     ILSSSNKNFSGAITLYYDWNNEEDKALATQYGFICDTRRCTWMLDGLTGSIHQKNKKA
                     DYSNVMVFHQPFTVGFYEYKATDGVPRGLVNALLPVTLTLDIVTSPLQFLILCTTRNC
                     "
     gene            complement(26804..27616)
                     /locus_tag="IU325_06305"
     CDS             complement(26804..27616)
                     /locus_tag="IU325_06305"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP5808653.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(27731..28138)
                     /locus_tag="IU325_06310"
     CDS             complement(27731..28138)
                     /locus_tag="IU325_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HAP5808654.1"
                     /translation="MIIMGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQS
                     GGLGAILSTWLSNQQGNQPVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPK
                     IIDALSPQGEVTTQANNDLLSAGMELLKGKLFR"
     gene            complement(28369..30783)
                     /gene="gyrB"
                     /locus_tag="IU325_06315"
     CDS             complement(28369..30783)
                     /gene="gyrB"
                     /locus_tag="IU325_06315"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP5808655.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(30812..31885)
                     /gene="recF"
                     /locus_tag="IU325_06320"
     CDS             complement(30812..31885)
                     /gene="recF"
                     /locus_tag="IU325_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP5808656.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(31885..32985)
                     /gene="dnaN"
                     /locus_tag="IU325_06325"
     CDS             complement(31885..32985)
                     /gene="dnaN"
                     /locus_tag="IU325_06325"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP5808657.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(32990..34393)
                     /gene="dnaA"
                     /locus_tag="IU325_06330"
     CDS             complement(32990..34393)
                     /gene="dnaA"
                     /locus_tag="IU325_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP5808658.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            34690..34770
                     /locus_tag="IU325_06335"
     CDS             34690..34770
                     /locus_tag="IU325_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808659.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTRQPV"
     gene            35000..35140
                     /gene="rpmH"
                     /locus_tag="IU325_06340"
     CDS             35000..35140
                     /gene="rpmH"
                     /locus_tag="IU325_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP5808660.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            35157..35516
                     /gene="rnpA"
                     /locus_tag="IU325_06345"
     CDS             35157..35516
                     /gene="rnpA"
                     /locus_tag="IU325_06345"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP5808661.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            35480..35737
                     /gene="yidD"
                     /locus_tag="IU325_06350"
     CDS             35480..35737
                     /gene="yidD"
                     /locus_tag="IU325_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP5808662.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            35740..37386
                     /gene="yidC"
                     /locus_tag="IU325_06355"
     CDS             35740..37386
                     /gene="yidC"
                     /locus_tag="IU325_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP5808663.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            37492..38856
                     /gene="mnmE"
                     /locus_tag="IU325_06360"
     CDS             37492..38856
                     /gene="mnmE"
                     /locus_tag="IU325_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP5808664.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         9494 a        10565 c        10049 g         9554 t
ORIGIN      
        1 attttagtag caccattttg ttgattttgt tcttttatag ttctatgagc atccataatt
       61 atggaatcaa aaaaaacatc tgttctttta aaactttcct gaacaggtac atatccttca
      121 tctttttgaa ctcgcaggct taataaatca tatatattac ttaaaattcc cgcaggaatt
      181 tcgtaattaa agtctgtatt attgataaca atactattaa ctaatttctt tctcaaaacc
      241 gtgtcagcgg aatgtgtttc taacgcagca gttttttcag acaacgattt gataatagtc
      301 gctacagcca ctcctgaata tggagtttct tttcgtaaac gtttatctct accaaccaga
      361 gtaatattaa atgacttatc acctgtttgt ttgatttcga tatactcatc ctctccagtt
      421 gccgccatcc aggtacgatt gccattattt tcagtgcctg gcatagggtg aaaatagtta
      481 agtatttcgg tattaacact gcgagaaccg gacataatat ttgctattaa gcgttttatt
      541 tcctgtaaca tccattttgc gaaattactg ccaggtgaat attttttctc gttatttgtg
      601 atattaagct cagcagcaat aattctattt ttatccaaaa aggtggaggg gcagggattt
      661 gacatcattg ttccgctgtt tatagggctt gccataatat acctcacgat gaaccattaa
      721 ttaatagaat gctggtcatg accactggta tagacagcaa tcagaattta acaccatcgc
      781 tggaaatttt tagccctaac tgtcaacccc ctggttatcg gtgcagcgac ttaaattaac
      841 gtcctgggct aataaattgt gcctgagatg gtctctggca caatttcacc ctatatatct
      901 gaacggatct atcaaaaatc gctcatcagt aaaccccccg agcggtttaa tgtcgctcct
      961 tactgaaaaa acaccgcaat cttgttaaac attgtcggat cggactgatt acgctgcact
     1021 ttcaccacat cttccagctt atcgatttgg cttatcatct gctccagacg ctggtcgtca
     1081 ttgaccagta gccagatatg gcttttgtcg ctgtcctgaa tcggcaggca gagaatgcct
     1141 tcgacgttaa aagcgcgacg ggcaaaaagg ccacaaacgt gggtcattac gcccggatgg
     1201 ttgcgaacgg tgagttcgag aatcacgttg tcatgagttg tgttttgcat ggcttattcc
     1261 cccaccattt cagtattcgc cgcacctggc ggcaccatcg ggtacacttt ttcttcggca
     1321 tcaatgcgca catggatcag cgccggacca gggcgattga tgatttcctg caatgcagcc
     1381 tgcggatcgg cttcgttatt caaatcacag gtttcgaggc cgaatccggc ggcaatctgc
     1441 ataaagttga tttttcccgg ataggtagcg gcaaaaacgc cctgcttgta gaacagactc
     1501 tgttgctgat gtaccagccc cagcgcttcg ttgttcatca gaatgatttt gacatccagc
     1561 tgattttcac tggcggtcgc catctcctga atattcatca tcaggctgcc gtcgccggag
     1621 aagcacaaca ctttgcgatc cgggttcgcc agcgccgcgc caatcgccgc aggcagacca
     1681 aagcccatcg tgcccagccc gccggaggtc agccactggc gtgggcgatt gagcggataa
     1741 gcctgcgccg tccacatctg atgctgaccc acatcggtgg tgataatcgc attgtcatcg
     1801 acacaggcgg caacggcgtt gatcaggccg taatggctta atggatcgca cgctttcggg
     1861 attggacacg ggaattcgcg ctgcaaatcc gctaccaact ggtgccactc tgcacgcggt
     1921 tgcgcttcca cctgcgggat cagctgcgcc agcacgtcat caacatccgc ctgaatcgct
     1981 acgtgcggct gcttgatttt acccagctct gcgcgatcga tatcgacatg aataattttc
     2041 gcattcggac agaactgctc ggttttgcca atcgcccggt catcaaaacg cgcgccgaga
     2101 acaatcagca gatccgcctc ctgcaagata tagttggtgc tgcgcacacc gtgcatcccc
     2161 agcataccca gcgacagcgg atgcgctttt ggcagcatac ccagcgccat taaagtcatg
     2221 gtggtaggca gctgcgcttt ctccgccagt tcacgcactc gcgcaggcgc attgatcaca
     2281 ccaccgccca gataaagcac cgggcgtttg gcagcattaa tcattgccgc tgcgtcatga
     2341 atgctttctt cgctaaaggc gggcgcgacg gctttttctg ccaccgcggg ctgtgcttca
     2401 atctcaaaaa cggccgtttg cacatcctta ggaatgtcta tccacaccgg gccagggcgg
     2461 cctgattgcg caatacggaa ggcatcgctc atgacctgcg ggagttcttc gatatgtctg
     2521 accagatagt tgtgtttggt gatggggata gagatgccgt aggtgtccac ttcctggaag
     2581 gcgtcggtgc cgatcattga ggcgggtacc tgaccagtga tgcaaatcag cgggatggag
     2641 tccagccgcg catcggcaat ggcggtcacc aggttagtcg cccccggtcc gctacaggcc
     2701 atacagaccg ccggtttacc gtcggtgcgc gccattccct gagcgataaa ccccgcgccc
     2761 tgttcatggc gagccagaat atggcggatt tgcgtgcttt ggcttaaggc gtcgtaaaca
     2821 gggaggatag aaccgcccgg aatgcccgtc acaatcttaa tgccctgctg ttccaggaaa
     2881 tgaacgataa attctgcgcc ggtaaagcgc ttatgcgtcg atgttgtgcc cgaacttgcc
     2941 atgctccagt ccttttcttc tgggccgact ttccgggagg tgattaaacg aaaaaccccg
     3001 cccggtttgc gccggcgggg ttttggaatc gtgtgttgtt ccagtcccta cggcgcattg
     3061 ccgacgacca ccaccacacg cacgacgacc actgcggcgg atggcgcagt tggtagtagt
     3121 tttgcgttga gcatggaagt agtcattggg gaccttgttg gttttgtgtt taacaatatt
     3181 tatacaagca cagctttaca ggggagacaa tggaaaattt ttcagcaagg gaaaattgag
     3241 gggttgatca cgttttgtac tgtactgcag gtaacaaaaa accccgccgg agcgaggttt
     3301 cgtcagccgc ccgcggctgg taaccgcaaa gcacactgta ttatgtcaac acagaaagta
     3361 tacgtgttcc gcgcaaaacg cgcaatttcg gcacgaattt tgacgtattt agtgcatagt
     3421 tgagtatcga tcacagtttg cgttttgtcc aaatattact gtttatttat acagtaaact
     3481 tctataatat cactgtacgc aatgtgttat gcgggggccg catcgttacc cggcgcacta
     3541 agtcctggct gaaacgggtg gtgccgtcag cgccttaacc ccgcgtgagc acactgtgtt
     3601 atgtcaacga gcacaacgtt tctccttgag ataccgcgtg cacaacagct ggcaacaggc
     3661 agcggaaagg tacgtcagct ggcagtgctc ctgaaccaca ggagacgcgt atgagcctgg
     3721 tggatatcgc cattcttatc ctcaaactca ttgttgcagc actgcaactg cttgatgctg
     3781 ttctgaaata cctgaagtaa ttcagattca agtcgcacca aaggggagcg ggtaaccgct
     3841 cccctttcat atttagcgtg cgggttggtg tcggatgcga tgctgatgca tcttatccgc
     3901 cctaccatcc ctcccggcaa catttattgc cgcttttgtt tacatattct gccgctaaac
     3961 aattccccat tcctggcgta tatctggcta acatttatca atgtgataga ttcctctccc
     4021 gcatttatgg gaatgcgtag tgacttattc taattatttt tataaaagca tccgtgataa
     4081 tgaaaaggca tagaaacgtc aatttgttat tgatgttggt attactcgtg gccgtcggtc
     4141 agatggcgca aaccatttat attccagcta ttgccgatat ggcgcgcgat ctcaacgtcc
     4201 gtgaaggggc ggtgcagagc gtaatgggcg cttatctgct gacttacggt gtctcacagc
     4261 tgttttatgg cccgatttcc gaccgtgtgg gtcgccgacc ggtgatcctc gtcggaatgt
     4321 ccatttttat gctggcaacg ctggtcgcgg tcacgacctc cagtttgaca gtattgattg
     4381 ccgccagcgc gatgcagggg atgggcaccg gcgttggcgg cgtaatggcg cgtactttgc
     4441 cgcgtgattt atatgaacgg acacaattgc gccatgctaa tagcctgtta aacatgggaa
     4501 ttcttgtcag tccgttgctc gcaccgctaa tcggcggtct gctggatacg atgtggaact
     4561 ggcgcgcctg ttatctcttt ttgttggtac tttgtgccgg tgtgaccttc agtatggccc
     4621 gctggatgcc ggaaacgcgt ccggtcgacg caccgcgcac acgcctgctt accagttata
     4681 aaacgctttt cggtaacagc ggttttaact gttatttgct gatgctgatt ggcggtctgg
     4741 ccgggattgc cgcctttgaa gcctgctccg gcgtgctgat gggcgcggta ttagggctga
     4801 gcagtatgac ggtcagtatt ttgtttattc tgccgattcc ggcggcattt tttggggcat
     4861 ggtttgccgg acgtcctaat aaacgcttct caacgttgat gtggcagtcg gttatctgct
     4921 gcctgctggc tggcttgctg atgtggatcc ccgactggtt tggcgtgatg aatgtctgga
     4981 cgctgctcgt tcccgccgcg ctgttctttt tcggtgccgg gatgctgttt ccgctggcga
     5041 ccagcggcgc aatggaaccg ttccccttcc tggcgggcac cgcgggcgcg ctggtcggcg
     5101 gtctgcaaaa cattggttcc ggcgtgctgg cgtcgctctc tgcgatgttg ccgcaaaccg
     5161 gtcagggcag cctggggttg ttgatgacct taatgggatt gttgatcgtg ctgtgctggc
     5221 taccgctggc gacgcggatg tcgcatcagg ggcagcctgt ttaagctcac gtcaccgcag
     5281 catcgtcatc agctccatgg gagaacgatg ctgctttatc agatcacgca tcacccgcat
     5341 atgcggtgcg gagtaagaat aaaacgcctg atagcccgca catagcacgc tgtctggcgt
     5401 ctcctcccga gcgtgcgggc acagccgatg ccagtcgcac gcctggcagc cacggcgcga
     5461 gtgcgggttt tcgctgccac accatttttg taatgtggcg tcaaaaagct caatcgagat
     5521 acgactgata tctgcccgca cccaacactc aaacagggca attaaaaatt tcccccacag
     5581 ctctgctgtc accgaagcgg ccgtcagatc gccccggtca tcaaattcaa gatggggaat
     5641 gagctgtacg gagcggatgc cccgctgttt gaaattgtga taaaaatcgg caggttgcag
     5701 agcatcctta tactctacaa ccagtttatg cctgtcctct tcagcatcaa tgacctgact
     5761 tcctgtcatg cgactctctc aataaatacg ataagttgat gtatatgcgt tacagcaaac
     5821 agtattgcga gagataacac cgcgagggag atcaaaaatt tgtcacggac tacgtgaaat
     5881 tgggaaaaat cgctattcgt gacatccatt aaattgcgat tacgagtgta gtgccagagg
     5941 atcagcgcca cgatggcgag gatgccaatc gaaatccaga ataacatgcc cgcctggtgc
     6001 cagttgtgtt tgatagccaa cgccatcaac gcgccgtagc ccagcatggt acgaaaccac
     6061 gccagtgatg tgcgctccgg ctgcagccct gggtcggcaa tgcgtctggc tttgcggcta
     6121 tccggcatac aacaccagtc ccatgacgat caccgccacg atcatcaata ttaaactgat
     6181 gattaataag ctgttggtgt aaggcaggtc ctctttcaga cgcatcgctt tttcattgcg
     6241 taaccagcgc aagtagccat acatcgctaa cccgccggaa aacagacaca acaacaacgc
     6301 cagcagttcg cgaatgacgg gcgtggcgaa atctggcgca agttggtcaa gcccaacccc
     6361 cgcagccaga aatcctagtg cggtacggat ccacgccaga aaagtacgct cattagccag
     6421 cgagaagcgg taatcgggcg cttctccgag gcgggaaatt ttcattgttg ctcctttgca
     6481 atttcactaa cgctctacct ttagcggttt ttttaagaat ttcgtccacc cgtttcctga
     6541 tatgcttttg ccatccgcta caaggaggtc agatgggtat catagcgcaa aataaaatca
     6601 gttcattggg catgctgttc ggtgccatcg ctttaataat ggggattatc cacttttcat
     6661 tcggcccttt ttccgcacca ccgccaacgc ttgaaagcat cgtggcggac aaaacggctg
     6721 aaattaaaag aggtcttctc gccggaataa aaggcgaaaa aattaccacc gtggagaaaa
     6781 aagaggacgt ggatgtcgat aaaatcctcg atcaaagcgg tatcgcactg gccattgctg
     6841 cgctactttg cgcatttgtt ggcggtatgc gtaaggaaaa tcgctggggc attcgcggcg
     6901 cgctggtttt cggcggcggg acgctggctt tccatacctt gctttttggt atcggcatcg
     6961 tgtgcggcct tctgctgatg ttcctgattt tttctttcct gaccggcggt tcgctggctt
     7021 aagcagcctg aataatgccc gccgggtgat catcagcggg cattatcgtc agaacttctg
     7081 ttttttctct tccactttca ctccttgcgt cggtaggccg gtgtcatagt cgcgtaccac
     7141 tggcgaatag ccatcttgtg ggcgtggacg aaatgccccc atccagcgcg gctgtgcatc
     7201 tttacgccac ggacgcaggc tccattgata actgcggaac ggatcgcgaa ttttgtccat
     7261 gtaatccaat aaggcgtcat gcattttgcg gcgaacgtcg gcaaaacgga tatcatcgat
     7321 cagattatgc atttcattta ggtcattacg tcgatcgtaa agttcatcac tggtgaagag
     7381 gttgagtacc agtttaaagt catccgtcac ccagcaacgc accggaataa aaccgccaaa
     7441 gctgtcatgc tcaatctcgt agcggttaaa ttccaccatt acgccgcgtg gctctttcgc
     7501 ggcaaggata ttttcccccg gcagaatctc tggtttttca atatttgcca gcgccatcat
     7561 tgtcggcagt aaatcgatat gactgactgg cgtatcgacc tgtcgccgct ccccttgcgg
     7621 cgaacggatg atcagcggaa tacgggtgat gtcgtcatac atcgccgctc ctttactgat
     7681 cagcttatgt gcgcccatca tttcaccgtg atcggaggta taaatgaccc acgtattttc
     7741 acgttgctct ggcgttaagg cattgatgac acgtccgatt tggtcatcaa caaagtcatt
     7801 gcaggcaaaa tagagcggat ggtgataaag cccgtcatca ccgactggcg atggcatcgc
     7861 ctgcgcccat aagcggtgat gttccggttt gttcgccagg tcatcctgtg ctttctcgcc
     7921 cagctcgtag taaaaatcag cgtatttctc taaatactcc accggacagg tgaacgggtg
     7981 atgcggctca tcatacgaaa ccaccatcag gaagggttcc tcggcgcgcg cgggctgttg
     8041 cagaaaatcc actgctcgat tgctgatgcg gtgcgcccag gtgaaggttt cgtcaatatg
     8101 gttcgcctgt aaatcttcga cgctgtttag gccattgcgc cacaggctaa tttctttttc
     8161 cgtcagttcg ctaagatagt tcgccccatc gaaccagtaa tcagcgtccc actccggcgg
     8221 acactcgcca gtgccgaaat agtcatgacc gtcgagatgc catttgccga tgtaacaggt
     8281 gtgatagccg gcatccttaa agtagcgccc catagtggag atgtttttgc ccggcgcgac
     8341 gttgttggtc cacgggccgg actggttagc gtagataccg gtaaacagcc cggcgcgcgc
     8401 tggtgtacaa accggtgaac aggtgtaggc ggaattaaag cgaatacttt ccgccgccag
     8461 actatcaata ttttgcgtat tcagcggctt accgctatag caaccgacca tattggtggc
     8521 ctgggtatcg gtcatgatga acagaaaatt ggggcgtttc ataggctatc cttcgcgtcg
     8581 ggagtaaagg ttacggctgg gtcgtgacga tgccgccagc tgtcaaaaat caggtagaca
     8641 atcactacgg cggcaatgaa ataactgatc atcgctaacc agcgcgtacc gtagccgccg
     8701 aattcagcca gcccggcata gacgccaatc atcgcgaaca ggatgccaat tgacgcgatc
     8761 ttgacgtttt tccacggttt catgtccact gaaaacgcat ctttgaaggt gaacggcgtg
     8821 gcgcgcggtt tgataaaacc gatcaccagc atcacgacca cgttgatgca gaacgtacag
     8881 gccagcacat agaggaaatg gaagtcgaac ttcaccagat agttgatggt gatgtagctg
     8941 attatgccaa tccccatcgc cacttttgcc gccagcgccg ggatgcgcgg gaagaaaaag
     9001 cccataatga tgatggtaac cagcggcacg ttgtaaatac cgttgagctg tttcatccag
     9061 ctatacaacc cctgcggcgc gttggcaatc cacggcgcga ccagcaccga aacgatggcg
     9121 ataaagaaac caaatttgcg cccaacggtg accagctgtt gcggctcggc attctggtta
     9181 atgatgcgac ggtaaatacc catactgaat aacgtactgg cgctattcag aaagccgttg
     9241 aaggtactga tcaccgcacc aaataacacc gcgccgaaga aacccaccag tggcactggc
     9301 agaacgttat tgaccagcgt cgggtaggcc atgtcggctt tcggtagatc ctgatacaga
     9361 tgaaacgcaa tcaaccctgg cagcaccagt accagcgggt cgagcatttt cagcaccgcc
     9421 gttaacagcg cccccttttg cccttccgcc aggctttttg acgccagcgt gcgttgcacg
     9481 atgccctgat ttgtacacca gtaaaaggtg ttcaccagaa ttaaaccggt aaatgccgcg
     9541 ccaatcggca agggatcggt cgggccgccg actgagttta atttctcggc gtgaacggtg
     9601 gtgagttgct caatgccctg cataaagctg cccttgccca tcgcgatcag gccaaatatc
     9661 ggcaccatca acccgccgat aaccagacca ataccgttga tggagtccgc cactgccatt
     9721 gcgcgcagtc cgccgatcac cgcatacaaa attcccgcca gaccaagcaa aattaccaat
     9781 agccagatag ccgcaccgtg agaaatctgt agcgattccc cgacgtgaaa caggctgttg
     9841 agcgccaacg cgccggagta gagaacaatc ggcagaaagc agacgccggt ggcaatgagg
     9901 aagcagaagt cgatgataat acgcgtcgtt ttatcataac gttcctccag aaaatcgggg
     9961 atggtggcaa tgccgcgctt cagatagcgc ggtaaaaaga ttagcgcgag gaagatcagc
    10021 gtcaccgctg aagtcacttc ccagcccatc accgacatgc cgcttttgta ggcctgcccg
    10081 gaaagtccaa ccagttgttc cgtggaaagg ttggttaaca ttaacgaagc ggcaataacc
    10141 ggcgctttta gtgaacgtcc ggcaagaaaa tagccttgtt gcgatccggt atctgttttg
    10201 cgaaccttcc accaggtgat gatcgccacc agcagcgtaa aaccgacaaa actcaagatt
    10261 tgtaacgaat tcatctcata gcccttattt atgtttatgt cccggacgca tgaatatagg
    10321 gagaggtaaa atgcgtcggg tcaggcagtc agattcttaa aatacaaacg tcgtatccct
    10381 gaacggattg ttttctgtta agttcaggtt gtgagcataa ttcgccggag ggatattaaa
    10441 atgaatggaa aattgcaaag ttcagatgta aaaaacgaaa ctccgtacaa tattccctta
    10501 ttgattaacg aaaatgtgat ctccagcgga atttctctga tctcgctctg gcatacctac
    10561 gccgacgagc attaccgggt gatctggccg cgggacaaga agaaaccgct tatcgccaac
    10621 tcatgggttg cggtgtatac cgtacaagga tgtgggaaaa ttcttttaaa gaatggtgaa
    10681 caaataactc tgcatggcaa ctgtattata tttttaaagc caatggatat tcactcttat
    10741 cactgtgaag gtttagtctg ggaacagtac tggatggaat ttacccccac cagtatgatg
    10801 gatattcccg tcggtcagca aagcgttatt tataatggcg aaatttataa tcaggaactc
    10861 accgaagttg ctgagttaat tacttcacca gaagcaataa aaaataatct ggcagtcgct
    10921 tttctgacga aaattattta tcagtggatt tgtcttatgt actcagacgg taaaaaagat
    10981 ccacaacggc ggcaaattga aaaactaatt gccactttac atgccagtct gcaacaacgc
    11041 tggagcgtag ctgatatggc tgccacgatc ccctgtagcg aagcctggtt gcgtcgtctg
    11101 tttttacgct ataccggcaa gacgccgaaa gaatattacc tcgatgcacg tctggatctg
    11161 gcgctatcgc tattaaagca acaagggaac tcggttggcg aagtcgctga tacgctcaac
    11221 ttcttcgact cctttcattt cagcaaagcc tttaaacata aatttggtta tgcgccgtca
    11281 gccgtgctaa agaatacgga ccagcaccca acggatgcca gtccacacaa ttaacgtaac
    11341 tctggccagt acgctttgtt ggcctccagc aaatcatcca gaatagcttt ggcgaccgcg
    11401 gcgctcggta ccgttttcga caaagtaatc gcctgccaca ggtgctgata tgaacgctgt
    11461 tcccaggcgt ccacgaccag tttttccacc gccacttgct gactcattaa ccctttctga
    11521 aactgcggga tatcgccgac caccagtggt tctggtccgt tgtgcccgac cagacacggg
    11581 atctcgacca tcgcttcatc atcaaagtta tggattgccc cgttgttagg cacaatcagc
    11641 aacatccgct cctgagtgtt gaaggcaatt gccgccgcca gatcgacgat gtatgacgca
    11701 tgttcgtcaa tttccagctt gcccgccgct gaatttcctg ccgccgtaat ggcgcggcaa
    11761 gcatcgaaaa cctgtttctc gcgatgctcc atcacctcat tcgcgcgggt atgttcaggg
    11821 ttggaatgct gaacgacata atccgggaag agataatatt tcagataggt gttcggcaat
    11881 gtatccgggt ccagtgcctg gacatcccgc gcttttgcat aggtgtcgtt ccagctcgct
    11941 tcagtatgtg tatcttgctg cggtggaaca taaccgtgct tcgagacata ttgccgcagc
    12001 tggggcatca ggtcgttgcc ctgcaaatct tcaattgatg tccaccagcc aaagtggttc
    12061 aggccgtagt agcgcacgcg catctgtttg cgatcttgca gcccaacaat ttgcgccatc
    12121 cggctttcaa taccgattgg catgtcacag atgttgagga ttttcgcatt cgggcgcaga
    12181 cgacgcgtgg cttctgcgac aatggctgcc gggttggagt agttgagcat ccaggcattt
    12241 ggtgaatatt tttccatata atccaccagt tccaggacgc cgccaatgga acgcatgccg
    12301 tacgctattc cgccgggtcc gcaagtttcc tgtccaacaa cgccgtggcg cagcgggatt
    12361 ttttcatcca gttcgcgcat cgggtatttg ccgacgcgga tgtgcgccat gacaaaatca
    12421 acgtcgctga acgccacttc aggatcggtg gtgtaactaa acgcaatgtc cggcgctctc
    12481 tctttaagga tgactttaca ggcttcagca atcacttcct gccgcgcacc atcgttatca
    12541 taaaatttca gtgcacgaag cgggaaacgg tcctgattcg ccaggagcat caacacgatg
    12601 cctggcgtaa aggtgcttcc accgcctgcg acaaccactg agaatttggt cattatactg
    12661 cctccgtaat ggcaacattt tctgctgatt gatgagaatt aattaagcta tcgagctgtt
    12721 cacgtagctg ggatacatgc agaccaatga tcacctgaat ggcatcgcca ctacggaaga
    12781 cgccgtgcgc tcccagcttt ttaaagactt cgtcatccag cgtttgtgac atgtcatgca
    12841 gtgcaatccg tagacgcgtc gcgcaattgt taatgctgga gatattgccg accccgccca
    12901 gggcttgcag gataccggca gcctgatcca gctctttttt tggctctgcc gcggtggttt
    12961 ggcctcgcga ggctttgtat tcggcttttg agtagagttt cacttccgca tcttcacgtc
    13021 ccggcgtgca catgttgaat tgcagaatca gggtgcggaa aaccacgaag tacagcaggg
    13081 taaagcacaa cccaatggcg atttgggtca gcatcatatc cgcgtggttg ctgaacatcg
    13141 ggatccagtt ttgcggtaaa acctggtcaa tcaaaccacc gcccatgttg cccaccacac
    13201 caaagaggta catcacggtc gacattgagg ccgcaagcac agcgtgtacc gcaaacagca
    13261 acggtgaaat gaacaggaag gtaaattcca gcggttcggt aattcccacc agcatggcgg
    13321 ttaaggttgc gggaatcagc aagcccgcca cttttacccg attttccggt gcggcagtga
    13381 agtacatcgc taaagaaatg cccacggcac caaagatttt tgagttaccg tgcagggcaa
    13441 aaccgccttc cgggaacaac gatttcagcg gctcggcgct cagactgaac tcttgcagat
    13501 gctgcgccca gtacatctga atgccgcctt caacagctgc cggaccaaag ataaacggtc
    13561 cgtagatgaa gtggtgtaaa ccggttggga tcagaatacg ttcgaggaag gtgtaaaccc
    13621 agaccccaag cgcacccgcc gaacgcagga acgcttgcag agattcaatc cccatttgta
    13681 cttttggcca gccgagcaac gtcagccagg cgcaggggat catcaccagg aaggcgataa
    13741 tcaccacata agatgtcccc tggaaaatgc cgagaaacac cggcagtttt ttatcgaaca
    13801 gacggttatg cagcgccgtc acaatgccgg aaattataat cgcgccgata atgctggtat
    13861 cgagggtttt aatccccgcc atcattgtca gcccgctacc tgccaccgcg tcctgagtga
    13921 aatcgacgcc gaagtagctt ccccaggtca ttcccatcgc gttgatgaaa tagttccagg
    13981 tcaggaaact caccatcacc gccagacaag cacgcccctg cgcttgcttc gcaaggccaa
    14041 tgggtaaacc gacagcaaaa atcagcggca tattacggaa taccgtccaa ccgccctctt
    14101 caataatgtg tacgatttgc gcgaataaac tgttcggatc ggtcagtgat tccccgacaa
    14161 acatcgggtt ttgcagcaag atggcaagac ccaccacaat cccggcgaag ggaaacagca
    14221 gcactggcgt gaacatcgcg ccgccaaagc gttgaatttg actgagcatt tccacatcct
    14281 cttttctcaa ttctgtaagt aggaagcctg attggttatg cggcaggcct ggagcgagga
    14341 taggaagatg cgatcggcag aacatgacga cccaatggga ttcgtgatcg ctttcatgct
    14401 tttcgttggg cggtagagat ctacgttttc gcgaaaaagt agacatctgt gagcactggt
    14461 acacagcatc gaacacatct ttaaaaaaaa gatttttttt caatcgatta agcagaactt
    14521 gtgggcgcat tacccgggct tgcaggcaaa aaagagatct agagatgatc tacaaaagca
    14581 ttgcggagcg gttaagaatt cgacttaact ccgcggattt cacgctaaat agccttcttc
    14641 ccggtgaaaa aaagctggcg gaagagtttg cggtatcgcg gatgaccatc cgtaaagcca
    14701 ttgatctgct ggtagcgtgg gggctggtgg tccgccgcca cggcagcggt acttacctgg
    14761 tgcgtaaaga tgtattgcat caaaccgcca gcctgaccgg gctggtggag gtgttaaaac
    14821 gccagggaaa aacggtcacc agccaggtgc tgatttttga aatcatgcct gcgcctccgg
    14881 ccattgccag ccagttacgg attcaaatca acgagcagat ctacttctcc cgtcgcgttc
    14941 gttttgtgga agggaaaccg ctgatgctgg aagacagcta tatgccggta aaactgttcc
    15001 gtaacctttc gctgcaacat ctggaagggt cgaagtttga atatattgaa caagagtgcg
    15061 ggattctgat tggcggtaat tatgaaagcc tgacgccggt gctcgccgat agactgctgg
    15121 cgcggcaaat gaaggtagcg gaacacacgc cactgctgcg gatcacctcg ttgtcatata
    15181 gcgagagcgg ggagtttttg aattattcag taatgttcag aaatgccaac gaataccagg
    15241 tggagtacca tttacggcga ctccacccgg aaaagagtta accgatactc cagaagagca
    15301 ccgccagtaa ttggggggtg ataatgcgca ggaacatcac taacggatag acagtggcgt
    15361 aagagagcgc cgccgcaccg ctcgttggat gaagattatt agcaaacgcc agcgccggtg
    15421 gatcggtcat ggagccagcc agcatcccgc acatggtcag gtaattcatt ttggctaaca
    15481 tccgcgccag aataccaaca gtaatcagcg gaacggcggt gatcagggca ccatatccaa
    15541 tccagcttag cccttcgcca ttgaccaggg tattcacaaa atccccacca gatttcagac
    15601 caacgaccga gaggaacagc acgatcccca gctcccgcag cgcgaggttg gcacttggcg
    15661 gcataaacca gtacagcttg ccgatactgc cgatacgccc gaggatcaac gccataatca
    15721 gcggaccgcc cgccagcccc agtttcaacg cggccgggaa tcccggcaca aagacgggaa
    15781 tagaacctaa caatacgcca agcccgatac caataaacac cggcagcatc tgtacctgtt
    15841 gcagtttttg ctgcgcattc cccagcacat tggcaacggc atcaattgcg gacggacgcc
    15901 ccaccaggtt gagaatatcg ccgaactgca ggctgatatc accactggcg accagttcga
    15961 ccccggcacg gttcaggcgc gagataacaa cgtcatagcg ttctttaaag tgcaggtcgc
    16021 gaatacgttt tccgagcacg ttttcattgg tgaccaccac acgctcgacg cgcaaatcag
    16081 tgcctttcgt agacagcgag gtatcgacct cctgaccaat caccagttgc gcattatgta
    16141 aatccgctgg ctgccccacc agatgcagca aatcgcccag ttggataatg gtatctggcg
    16201 aaggtacttt tagggtttct tcgcgtttca gacgcgagca gataattttg tcgccattga
    16261 gaatcggtac atctttaatg gctaaatcat gcaggttagg gttctcaacg cgaatattga
    16321 tagtcctgat cagcgcgccg ccattggtgc gtgaagactc gtgctgctgg gcttctgtct
    16381 cgacattgac gcggaaaata acccgcaaca tccacatggt gaacaaaatc ccgcaaatgc
    16441 cgaatggata cgccatcgcg taactcatcc ccatctgatc gaccatttcc attggtgtac
    16501 ccaggtcgcg caagatctgc tgccctgccc ccagtgctgg cgtattggtt accgcaccgg
    16561 agaaaatccc cagcactacc ggcagtggaa tatcaaacag tttatgcagg atggcggtaa
    16621 ccagaccacc gatgatgacg atcagaacag caaacaggtt gaggcgtaat ccggagacgc
    16681 gcaatgaggc aaagaagccc ggccctacct gaatcccgat ggtataaacg aacaggatca
    16741 ggccaaattc ctgaataaca tgcagcatat cgctacttaa tgtcatcccc gcctgagaaa
    16801 caaaatggcc gacgatgatc ccaccaaaca gcacgccgcc aatacctaat cctatgccgc
    16861 gaaatttgac gttgccgata aacaaaccga cgactgccac caaagccaga atactgaccg
    16921 ttaatgctat atcactcatc gtccatttct cttgagataa cattttaatt gctacggatt
    16981 atggctgaat cggtgaagca gatttgcctt atagcgcaca ttatcacggg aaagaacgtg
    17041 ccgaaatatc ttaaacaggc gaaaattaac gcattgttga agaagaaaga caaaaaaagc
    17101 cccaccagaa tggcggggca aagagaatag ctagttagct atttaacgcg ggacgttcgc
    17161 tgatagcgat acgctgcgct gcgatgggtt caggctcatt acgaattaaa tcaatatgca
    17221 gtaaaccgtt tacgaaggtt gcaccagaga cttccatatt ttcagccagc gtaaagctca
    17281 ggctaaatgg ctgattcata agcccttgat gcagccattt tttctcttct tttggctgct
    17341 ccggcgtgcc ttttacgctc aggcgcgtac cttccagttg aatctctaaa tcttcctgac
    17401 gaaaacctgc cagcgcaagg gtaatgcggt agtggttatc gtcgcttttc tcaatgttgt
    17461 acggcgggaa gctctggctt tcaccggcgt tttgcagtgc gttggccagt ttgtcaaaac
    17521 cgatccattg acgcatcagt ggggataaat cgaagttacg catagtcatt tctccttcta
    17581 agaagcgagt aagtacctgc aaatccgaag attcgcatat gctccctgac ggcgagcatg
    17641 gagatgtcag gccgcgccag gcggccttaa ggaattagtt gatttcgata cggcgcggtt
    17701 ttttcgcttc cggaatcacg cgttcgagat cgatatacag cagaccattc accaggttag
    17761 cgccacgaac atgaatgttc tcagctaact ggaatttgcg ttcaaagttg cgttcagcga
    17821 tgccctggta cagataggtg cgctcttttt gttcgtcggc gtgagcacct ttcaccacca
    17881 gcagattatc ctgggcggta atttccagtt cgctctcagc aaaaccagcc acagcgatag
    17941 caatgcggta atggttttcg tctaccagtt caacgttata cggagggtag ccgccattac
    18001 tctggctctg gttgttttct aagtggttaa acaaacggtc aaatccaata gcagaacggt
    18061 aaagcgggga taaatcaaag ttacgcataa tcaatagctc ctgaaatcag cgagaatgta
    18121 agaccttcca caatggacag gtcaggtagc cagaacaccc atcaggcgcg tcctcatcgg
    18181 ctacgatgta aaaatgggtc tggaaatgaa cttttcaagc cttatcagcg gacttttttt
    18241 tgcagtttat ggtctattgc atagactgag ggggcagcac gaaacgttaa agtgcgatag
    18301 ccgccacagt gcgacgtaat gatgatgtta ttttcagcaa gggatcgcta taactcatca
    18361 ggccaacacc cacgatcaac agatgaattt attatgataa gaaatgtgtt gttagcgttc
    18421 atgatatgca gcggaatgac attactcgga ggatgctcca gcgtgatgtc ccataccggg
    18481 gggaaagaag gaacgtatcc cggcacccgc gccagcgcca ccatgattgg cgacgatgaa
    18541 accaactggg gcactaagtc gctggcgatt ctcgatatgc cgtttaccgc tgtgatggac
    18601 acgcttttac tgccgtggga cgtgttccgc aaagacagct cggtgcgctc acgcgttgaa
    18661 aaaagcgaag ccaacgcgca agccaccaat gcggttattc caccggccag aatgcctgat
    18721 aactgattta gcgatcagtt tcggtgatcc acagctggcc gccttccatc cacgccagcc
    18781 actgaccatc cggcgagaag accacggcgt ccgcagaagg tggattggcg tggttttcgg
    18841 ttaaatactc aacctcgccg ctttgcgcat gggcgcaagc aattcgatta tccagcacaa
    18901 agcccaacca ttctcctgac ggatgccagt taaatgcaga ctgaatatcc gttttgttat
    18961 gggttaactg gcgcggctcg ccgccttgtg gcgagataag ccacagttgc acaatgccgt
    19021 tatcatcacg cattaaaaac gcgatttgcg taccctgcgg attacagcgc acccagtggc
    19081 gcgggacgtt aactaacccc ggataagccc gatggtgggt aaaggttaaa cgtcgctgca
    19141 cgacgccgcg cggtggcgcg ggcagggttg tttccgttcc acttaacggc gcatcacctg
    19201 ccgctttcca gccagcttca tcttgcggta actcaacgat aaatagctcc ggtactttct
    19261 cgccctttgg cgaaagtgtg tcgccaataa acgccagcgc gtgatttcct acccatcctt
    19321 cttcataagc acgattgatt tcatcgctgc caggctgcgg cgtgggcgtg gttttactca
    19381 ccagcacgca ccagtggcta ccgctgtatt cacgcggatg ctgcttttgt acgttgaccg
    19441 ggccaaacgg tgcagcaaca ccgacgtttc gcaaatccag cgccgggcag agttgatgca
    19501 ttacatggtc gttataggta aagctcaccc tttcaccgtt cgggctaaag acatgcacat
    19561 ggctgccgcc gcgcagcgct cctggcgtat acggcgcggt aatatccatc gcatcaaggt
    19621 tactcatctt gccgccttca acaatcactc cgcgccgatg atggaagtcg taatgccatg
    19681 tttcatcagg attttccggg ccgtggatga agacatactt ctctgacttc ggatgaacgg
    19741 tcaccacgcc gacgtgtgcg ccctgtgacg cgcgatagat aacctcgacc tcgccggtat
    19801 ggatattcac acgctcaatg gtctcgccgg taaacgacgc ccctgaagga cgaacgtcaa
    19861 ataccagcca ctggctgtcg ggcgtccagg tattggtatt ggtgagcagg tgattacggg
    19921 gagcaaaggt tatctgtttc atggcgcggt cctgatacaa aacggttccc gccatcatac
    19981 ttcacaagga cttcactttc agacaatagt ctttcgccat cgtgatgaat gtgagtgaaa
    20041 agatggaaca tttcgacgtg gcgattattg gcctcggccc ggcagggtcg gcgttggcac
    20101 gaaagttagc aggcaaaatg caggtgatcg cgctggataa aaagcaccag tgtggtactg
    20161 aaggtttcag caaaccttgc ggcggtctgc tggcaccgga cgcgcagcga tcttttattc
    20221 gcgatggact gacgcttcct gtcgatgtga tcgccaatcc acagattttc agcgtcaaaa
    20281 ctgtcgacgt tgccgcatcg ttcacgcgta actaccagcg aagctatatc aatattaatc
    20341 gccatgcttt cgacttgtgg atgaaatcgc tgatccccgc cagcgttgag gtttaccacg
    20401 acagcctgtg ccgaaaaatc tggcgtgagg atgataaatg gcatgtcatt tttcgtgcag
    20461 acggttggga gcagcatatt actgcccgct atctggtcgg tgccgatggc gcaaactcga
    20521 tggtgcggcg acatctctac ccggatcatc aaattcgtaa atatgtcgct atccagcagt
    20581 ggttcgcaga gaaacatccg gtgccgttct actcctgcat ctttgataat gcgataactg
    20641 actgttactc atggagtatc agcaaagacg gttattttat ctttggcggt gcctatccca
    20701 tgaaagacgg tcagacgcgt ttcacgacgc tgaaagagaa aatgaacgcc tttcagttcc
    20761 agtttggcaa agcggtgaaa agcgagaaat gtaccgtgct gtttccctca cgctggcagg
    20821 attttgtctg cggtaaggac aacgcctttc tgattggtga agcggcggga tttatcagcg
    20881 ccagttcgct ggaggggatt agctatgcgc tggatagcgc agagattctg cgttcggtgt
    20941 tactgaagca gccggagaag agcaacgccg cctactggcg cgccacccgc aaactgcgtt
    21001 taaaactctt cggcaaaata gtaaaaagcc gatgcctgac cgcaccggct ttaagaaagt
    21061 ggattatgcg cagtggtgtg gcgcatattc cacagttgaa agattagcca acgcgtttca
    21121 catcgcccac cagcaggatg tatgagagcg cgccaatcaa cgcgacggcg gagatataaa
    21181 ccagtgcagg cgcgaaaccg taaccctgcg ccaggtaacc caccaccagc ggaacggtga
    21241 tgccgcccag accaccggca aagttaaaca cgccgccggt taaaccaatc aggcgcatcg
    21301 gtgccagaga agagaccagc gaccaggtaa tcgaagcaaa accgttgccg aagaatgcca
    21361 gcgccatcag gcacataatc atcatcggat cgttagtgta gttagcgccc ataatgcagg
    21421 tggagatcag caagccgcag ataatcggcg ttttacgcgc aaagcccagt gaaaagccct
    21481 tacgtaccag cagatccgcg acccagccag agagcaggac gccgacaaac gccgcgagga
    21541 atggcaccgt ggtcataaag cctgctttca gcgccgtgat tcctttttcc tgggttaaat
    21601 agttcgggaa ccaggttaag aaaaaccaca gtgtagaagc caccgcaaat tgcccaagat
    21661 agacgccaat cagtttacga tggaacacca gtttccagtc tttggctgtt aacggctgac
    21721 gcgcctcttt cttcaccggc gcatcaccat ccaccagacc gccgccatca cgaatgtaat
    21781 ccagttcagc gttgctgatg cctttggtca ggcgcggcgg ctgataaacc ttaaaccaaa
    21841 tcagcgacca gataatgccg ataccaccgg tgacaatgaa cacccagtgc cagctcaaca
    21901 tctcctgaat ccagatcagc agcggagtca aaaacgccag accgacaaac tgaccagacg
    21961 tataaaaacc aacggcggaa gcgcgttcat gttccgggaa ccagctggtc accatccggt
    22021 tattggtcgg gaaagcgggc gcttcgaaaa taccggttat cgcgcgcaga ccaattaatg
    22081 acattaatcc cgtggcaaag ccctggaata acgtcgccac tgaccagcca aatatcgcaa
    22141 taaaataagt cacgcgagaa cctacgcgat ctaaaaacca accgccgggg atctggcata
    22201 acgtataaag ccaggcgaag gccgaaaata catagcccat ttccgctttc gtaataccga
    22261 actcttcctg aatatgggcg gaagccacgg ccaggttagc gcggtcaaca taacaaatga
    22321 ccaccgtaat aaagatcatc accagcgtca gataacgccg acgccccggc tttgctgcat
    22381 taacgggaat atccatagcg agctttctcc agattttggg catagcgaag ccgctcacca
    22441 tgccctgtaa tttacagagg gttatatttt tgtattgctg ttttagtgcc cgatgagggg
    22501 cttacgtggc aggaattacc actctgctac gctgttatct tcatgacgcc agagcggatt
    22561 acgccagtcc ggggcatttt tactgaactc aataacttta gcttcgtcga tttccacgcc
    22621 taagcccggt ttcgttaatg gtttaaagaa accgccgacc atgctgaagt cttctttgtt
    22681 tttcacaaag tcgagtaact ccgcgccttt gttgtaatga attcccatac tttgttcctg
    22741 aagtacggcg ttataggaaa caaagtcgat atgcagacaa gccgccagtg caatcggtcc
    22801 gagcggacag tgcggcgcaa gggtcacgtc ataggcttct gccattccgg caattttgta
    22861 gcattcggta ataccgcctg catgagagag atctggttgc agaatcgaaa taccgcccgc
    22921 ctccagcacg cgtttaaaat cgaagcgtga gaacatgcgt tcacccgccg ccagtggaat
    22981 atgcgtttgt gccgccagtt tcgggtagta ttcggcttgt tccgccagca ccggctcttc
    23041 aataaacagc gggcgatacg gctccagttc tttaatcagc actttcgcca tcggcgcgct
    23101 gacgcgaccg tggaaatcaa gaccaaactc aatctgattg ccaaaagctt cacgaatttg
    23161 tgccacggtg ttgactgccg catctaccgc gcgggagtta tcaattagcc ccagttcttc
    23221 acaaccgttc agtttgaagg tatcgaagcc gatttcgcgc agggttttaa tgccgtcgat
    23281 aacatccgcc ggacgatcgc cgccgaccca actgtaggct ttaattttgt cgcgaaccag
    23341 gccgcccatc agttgccaca ccggcgtatt cagcactttg cctttgatat cccataacgc
    23401 ctggtcgatc ccggcgatag cgctcatcag gattggaccg ccacgataaa atccggcgcg
    23461 atacatcact tgccataagt cattgatgcg cgatggatcc tgaccaatca aatagtcgct
    23521 cagttcgtga actgccgctt ccactgtacg ggcgcggcct tcgatcacgg gctcgcccca
    23581 gccgaccacg ccttcatcgg tttcaatttt caggaacatc cagcggggag gtaaacgata
    23641 cgtggtaatt ttggtgattt tcattgcact gcctctcgat acgccttaac aaatgctgct
    23701 gcctgctgcg cggtgcgttc tatggactgc ccggcgcgat agagatcgct gcctaagcct
    23761 gcccctgcac aacctgcgtc tatccactgc gccaggtttt ctggcgtcac gccgccaacg
    23821 gcaaagactg cgatgtccgg tggtaatacc gcttttaacg ctttgatgta ttgcggacca
    23881 aaagccgatg aagggaatat tttcagcgcc tgcgcacccg cgtcgagcgc ggtaaaggct
    23941 tcggtcgccg tcgcgcagcc ggggcaaacg gtcatgccgt agcccaccac acggcggatc
    24001 acttcactat ggatattggg cgtaacgatg agctggcagc ccatcctggc gagcacatcg
    24061 acctgttcag gtttcagtac cgtacctgcg ccaatcaacg ccttgtcgcc atacgcatca
    24121 acgatggcgg gaatgctctg ctcccattgt ggggaattca gcgggatttc aaccgcgtcg
    24181 aacccggcgt caatcaccgc gccaacatgc gccagcgcct cgtcgggcgt aatgccgcgc
    24241 aaaatggcga tcagcgggag tttagtttgc cactgcatga gcgatgctcc ttataccagc
    24301 ctgaaatgcc gtgtcgcccg ccaccgccgc cacatcgcaa cccatcgcct gaaaggcttg
    24361 ctggtagcgc gcggtcagcg atgttccggc gacaagggtg atggcgtgtt gatgggccac
    24421 atagtcgcgc atactggcaa cctctgcgcc aatcaacaaa ccagagagaa attcgctgac
    24481 ctgttctcgg ggaagtgttc ccagcacatg cgaggcgcga acttcaaaaa tctgcggcaa
    24541 tatggcggga gcattaaggc cacgctcaag accagccgcg aaggcatcgg cagagttttc
    24601 ctgcggcggc aaacctgcgc caatcaatga gtgatttaac agtaaatgat gtaattcacc
    24661 ggtcatcacg gtgcgaaaat cgttgatttg ctggctatcg gcctgcaccc atttgcaatg
    24721 cgtaccgggc atgacataaa gagaggaagg agccagagca cgcgcgccga tcaattgtgt
    24781 ttcttcgccg cgcatcacat tatggttatc gtcatgagag acgcataatc cgggaataat
    24841 ccagatattg tcgccaaccg acgttaattg ttcgccaata gacgaaaaac gggcaggaac
    24901 ggataaatac ggtgcaactt tccagccgac attgctgccg accattcctg ccattaccac
    24961 tggcgtattc tcttcacgcc agtcggtcgt cacttctgct aacaccgcag ccggagattt
    25021 tccgttcaga cgcgtgacgc ctgcttctga ttgcctgctc tccaggcagt ggtcgccatg
    25081 ataaagccag gcgcgcagat tggtcgatcc ccagtcaatt gcgatgtagc gagctgtcat
    25141 gtgatttcct ttaaccttcg tgtcgagcta gcgatcatgg taagcgccgc ctgctctgcc
    25201 gcatcgccgt cctgatgccg tatcgcatcg aacagcgcct tatgttcctg gagcgtttgc
    25261 ggcatgttgg cctcatcgcc catccaggtt cgttcaaaaa ccgcccgctg cagcgaactg
    25321 atcgcaatgc taagttgctg taacaccggg ttatgcaccg actgcagcac cgcctcgtgg
    25381 tagcgaatat ccgcttcgtt aaacgcttcg cggtcctgat tgttggcaat catctcgttc
    25441 agcgccgatt caatctgcgc cagatcgctg gaggtcgcgc gctctgctgc ccaacgggca
    25501 atcgccggtt ccaccagatt tcgcacttcg ctcatggcac tgataagtcg tgggtcgtag
    25561 tcgttttcca gcacccattg cagtacgtca gtgtcgaggt aattccactg gttacgcggt
    25621 gccacaaacg cgccgcgata acgtttcatt tcaatcagcc gcttcgccat cagcgaacgg
    25681 aacacctcac ggatgatgtt gcgcgaggtt gcaaactctt cacagagctc cgcctcagcc
    25741 ggaagcggcg agcctggtac gtatttgccg tgaacgatct gtttacccag cgtaatgaca
    25801 atgcgatcgg ttttattgag agtcatggag agtccttgtg ctctgtatgt tcttctctac
    25861 tttaccccga tcgatgcata acgcggcaac tttgtagtac tagcgtgatg acgttcgcgt
    25921 ttgccgtgtg cgtaatgtag tacaaactta tattgttgta ctacaattta gatcacaaaa
    25981 agaacaatgc ataaaaaaat gacatgcgtc gggcagaaat ctgaaaaggg atgtcaggcg
    26041 ctaaacagga gggaaagaag agtatgcttt caacggctta gctactcgtt taaaggatta
    26101 atcatgaagt tgaattttaa gggatttttt aaggctgccg gtttgtgttc tttggcattt
    26161 gcgttgaccg gatgtatcac ttggggattg gtttccaata ctgcaagccg tccacatgcc
    26221 gagcaatggc ggtcagacac gattaagggt ttatcgctgg cagaagatag taacggtacg
    26281 aaaggctatg tttttgttgg tgaatcgttg gactatctgt tgaccaccgg cggcgataag
    26341 gtggttaaaa tgctgaacga tccggcaatt catggtgaaa gaatcaccgt ttctgataac
    26401 gcgaaattta ttttaagcag cagtaataaa aatttctctg gtgctattac gctctattac
    26461 gactggaaca acgaagaaga taaggcactg gcaacacaat atggttttat ttgtgatacc
    26521 cggcgttgta catggatgct ggacggtttg acaggctcaa tccaccagaa aaataaaaaa
    26581 gcggattact caaacgtgat ggttttccat caacccttta cggtgggatt ttatgagtac
    26641 aaagcaacag atggcgtccc gcgcggcctc gttaatgcgc tgctgccagt cactcttacg
    26701 ctggatatag taacgtcacc actccagttc ctgattttat gcacgaccag aaattgttaa
    26761 tcgctgcggg caaacgcatt catgccgttt gcccgttcac tgattaattc agcacatact
    26821 tctcaatagc aaatgccacg ccatcttcaa ggttggattt ggtgacaaag ttcgccactt
    26881 ctttcaccga aggaatagcg ttatccatcg ccacaccgac gcctgcatat tcaatcattg
    26941 cgatgtcgtt ttcctgatcg ccaatcgcca tgatttcttc cggtttaata cctaacacgt
    27001 cggccagtga tttcacaccc gtacccttgt taacacgttt atcgaggatt tcgaggaagt
    27061 acggcgcact tttcagcacg gtatatttct ctttcacttc ctgcggaata cgcgcgatag
    27121 cctggtcgag gatggcgggt tcatcaatca tcatcacttt caggaactgg gtattggggt
    27181 ccattttctc cgcttcgcag aacaccagcg gaatggtggc aacgaaggat tcatgcaccg
    27241 tgtagtagct gatatcgcga tttgctgtat acagcgtggt gcggtccagg gcgtggaaat
    27301 gagaaccgac ttcgcgagag agtttttcca ggaaacggta gtcgtcatag ctgagagcgg
    27361 tttgcgccac ggtgctacca tcagcggcct tctgcaccag cgcaccgttg taagtaatgc
    27421 agtagtcgcc cggctgttcc atatgcagct ctttcaggta attgtgaaca cctgcataag
    27481 ggcgacccgt cgttagcacg acattcacgc cacgggcgcg agctgcagca atcgcatttt
    27541 taacggcagg tgaaatggtg tgatcgggca gcagaagggt gccatccata tcgatagcaa
    27601 tgagtttgat agccatgagt tccccaggta gattggttcc tgacccatgc taacgcgatt
    27661 ccgctcaaaa atcagtacaa cacccgaggg aaaaggggga tgcaacgcgc gttgcatgct
    27721 ccccttttgc ttagcggaag agtttccctt tcagcagttc catgcctgcg gaaagcagat
    27781 cgttattggc ttgtgttgtc acttcacctt gcggtgagag tgcatcaata atcttcggca
    27841 attgttctgc cagtaaactg gaagctgtac tggtatccac gcccagtttt tgcccgagat
    27901 cggacaccgc atttgtgccg agcgctgatt ccagttgctc gccgctaact ggttggttgc
    27961 cctgttgatt actcagccag gttgagagaa tggcacctaa accgccactt tgcagttttt
    28021 ccagcagcac ctgaatgccg ccctgctcct caacccagct caaaatggcc tgatatttcc
    28081 ccgcatcgcc tttcagaaag gcaccgacaa cttcatcaaa aagccccatg ataatcacct
    28141 gtaaagcgtt acgtgttgac tcaaaaagta tagatctgcg ggatgataat tgcggattgc
    28201 agaaataaaa agggcgaaga tgatctccgc ccttttctta tagcttcttg ccggatgcgg
    28261 cgttaacgtc tgatccggcc tacaaaatca tgcaaattca atatattgca cgagtctcgt
    28321 aggcctgata agcgtagcgc atcaggcacg ctcgcattgt taacgtcatt aaatatcgat
    28381 attcgccgct ttcagagcgt tctcttcaat aaacgcacgg cgcggttcaa cggcgtcgcc
    28441 cataagcgta gtgaacagct ggtcggcggc aatcgcatcc ttaacggtaa cgcgcagcat
    28501 acgacggctt tccgggtcca tggtggtttc ccacaactgt tccgggttca tctcgcccag
    28561 acctttataa cgctggatgg agaggccgcg acgggactct ttcaccagcc agtccagcgc
    28621 ctgctcgaag ctggctaccg gctgacgacg ttcgccacgt tcgataaacg catcctcttc
    28681 cagcaagcca cgcagtttct cacccagcgt gcagatacga cgatattcgc caccggtgat
    28741 aaactcgtga tccagcggat agtcagtatc cacaccgtgg gtacgcacgc gaacaatcgg
    28801 ttcaaacagg ttttgttcgg cattggtgtg gacatcaaac ttccactggc tgccgtgctg
    28861 ttctttgtcg ttcagttcgc tgaccagcgc gttcacccag cgggtaacgg tctgctcatc
    28921 agagaggtca gcttccgtca acgtcggctg atagataagc tctttcagca ttgctttcgg
    28981 ataacgacgc tccatacgat tgatcatttt ctgcgtcgcg ttgtactcag ataccagttt
    29041 ctctaacgct tcgccagcca atgccggtgc actggcgttg gtgtgcagcg ttgcgccgtc
    29101 caacgcgata gagatctggt actgatccat cgcttcgtcg tctttaatgt actgttcctg
    29161 cttacctttc ttcactttgt acagcggcgg ctgagcgatg tagacgtgac cgcgttcaac
    29221 gatttccggc atctgacgat agaagaaggt caacagcagc gtacgaatgt gcgagccgtc
    29281 gacgtccgca tcggtcatga tgatgatgct gtgataacgc agtttgtccg ggttgtactc
    29341 gtcacgaccg ataccacaac caagcgcggt gataagcgtc gccacttcct gagaagagag
    29401 catcttgtcg aagcgcgctt tctcgacgtt gaggatttta cccttcagcg gcagaatcgc
    29461 ctggttcttg cggttacgtc cctgcttcgc agagccgccc gcggagtccc cttccacaag
    29521 gtacagttcg gaaagcgccg gatcgcgttc ctggcagtct gccagtttgc ccggcaggcc
    29581 cgctaagtcg agcgcacctt tacggcgggt catttcacgc gcgcgacgcg ccgcttcacg
    29641 ggcacgggca gcatcgataa ttttgccaac cacgattttc gcgtcggttg ggttttccag
    29701 caggtattcc gccagcagtt cgttcatctg ctgttcaacc gccgatttca cctcagaaga
    29761 aaccagtttg tctttggtct gggaggagaa tttcgggtcc ggcactttca cggaaacgac
    29821 cgcaatcagg ccttcacgcg catcgtcacc ggtggcgctg actttggctt ttttgctgta
    29881 gccttctttg tccatgtagg cgttcagggt acgggtcatc gccgcacgga agcctgccag
    29941 gtgagtaccg ccgtcacgct gcggaatgtt gttggtaaag cagtagatgt tttcctggaa
    30001 gccatcgttc cactgcaacg ccacttcaac gccgatacca tctttttcgg tggagaagta
    30061 gaagatattc gggtggatcg gcgttttgtt cttgttcaga tattcaacga acgctttgat
    30121 gccgccttca tagtggaagt ggtcttcttt gccgtcacgc ttgtcgcgca gacgaatgga
    30181 aacgccggag ttgaggaacg acaactcacg cagacgtttc gccagaattt catattcgaa
    30241 ctcggtcaca ttggtgaagg tttcgaggct aggccagaaa cgcaccatgg tgccggtttt
    30301 ttcagtctcg ccggtaaccg ccagcggggc ctgcggtaca ccgtgttcgt agatctgacg
    30361 gtgaatttta ccctcgcgct ggataaccag ctccagtttt tgcgacaggg cgtttactac
    30421 cgaaacacca acgccgtgca gaccgccgga cactttatag gagttatcgt caaatttacc
    30481 gcctgcgtgc agaacggtca tgatcacttc cgccgccgat acgccctctt ccgggtgaat
    30541 accggtcgga atgccgcgcc cgtcatcctg tacagagaca gagttatcgg cgtgaatggt
    30601 gacgataatt tctttacagt gacccgcgag cgcttcgtcg atagcgttat ctaccacctc
    30661 gaataccatg tggtgcagac cggtgccgtc atccgtgtcg ccgatataca tacccgggcg
    30721 cttacgcacc gcatccagcc ctttcaggac tttgatactg gaggagtcat aagaattcga
    30781 catcaacgtt tctcgctcat ttatacttgg gttaatccgt tattttaccc ttttccacgg
    30841 taaacatctt cgaattttcg tccgacatgt ctataacgtg ttcagcactg atcgcgctga
    30901 caaagacctg tgattgcgtc gcttttaagc ggctggcaag cagcccgcga cgctcatcat
    30961 caagctcaga ggcaaaatca tctatcaggt agagacaccg ccgcccgctt tcacgggtga
    31021 ggaactctcc ttgcgccaga cgtaaggcgc acatcaacag cttaagctgc ccacgcgata
    31081 aggtatcttc caccggcgca ccgtcggcgc gaatgcgtaa gtccgctttg tgcgggccat
    31141 gcgcggtgta ggttagctgg cgatcgcgtt caaaattacg ttccagcacc tcagcgtact
    31201 ctgtctcttt ctcccagccg cgctggaaag agaaagtcag agaaaactca gggagaaatt
    31261 gcttacaggt atcagccata tcggccgcga taccggcgct atactccgcg cgccaggtgc
    31321 tgatttgctc cgccagcggg atcagttctt tatcccacgg gcgtagctgt tcgtaacgtg
    31381 tcacctggcg cagcgccgca ttgcgctgct tgagcaatcg cttgagattg ctccaggcgg
    31441 tgaaaaatcc aggttcgttg tgaaagcatc cccagtcgag gaatgctctt ctgtatttgg
    31501 ggccgccgtt gagtaaagta aacccttctg gtgttatcaa ctgcattggc atcaggtgcg
    31561 ccagttccgc gaccttatgc ccgtctgtac cgtcgatgcg gactttgctg tcgccctgtt
    31621 tgtctttggt taagccaatc gctgtctcgc gctcttcgcc ctgtaatcgc ccgtggagaa
    31681 caaacgcctc ctgctcgtgg cgaatgacgc gaccaatctg caaactgcga aacgcccgac
    31741 catggccgag cgtatagatg gcttccagca cgctggtttt gccactgccg ttggcaccta
    31801 ccagaaagtt aaagccggga gataaagcga gatccgcggt ttcgatattg cggaaatcgc
    31861 ggatcaacaa gcgggtgagg gacattacag tctcattggc atgacaacat aggcagccga
    31921 ctgtgatgcg gcatcttcaa tctgcacgct ggaaaccgaa tcggtcagca tcatgcgcac
    31981 gttttcacat ttcagcgcgt tcagaacatc cagcacatag ctgacgttga agccgatttc
    32041 catctccgca ccgctatagg taacgtcgag gatctcttcc gcttcttcct gttccgggtt
    32101 attggcggtg attttcagct ggttttcgct gacatacagg cgcacaccgc ggaatttctc
    32161 gttagagaga attgccgcgc gagcaaacgc ctgcttgagg agatcgcagc ctgcttccag
    32221 atgtttgtct gggttcttcg gcagaacgcg tcggtaatcc gggaagcgac catccaccag
    32281 tttcgaggtg aagataaagt cgccaacgtg ggcgcgaatg ttgttgctgc caatctgcac
    32341 gcgcagcgga ttgtcgccgc cgtcgagcat acgcatcagt tcaatcacgc ctttacgcgg
    32401 cacgatcacc gaatggcttg gcaaagattg accaattggc atggagcaga ccgccaggcg
    32461 gtggccgtcg gtcgccacgg tgcgcagttc ttcgccttcg gtttcaaaca gcataccgtt
    32521 taagtaatag cgaacgtcct gatgcgccat agaaaactgg gtcgcttcaa tcagacgctt
    32581 catcgtcgcc tgcggcaggg taaattcgac ttcgctctgc cagtcatcga ggttcgggaa
    32641 atccgcggct ggcagggtag acagcgaaaa acggctacgc ccggagcgca ccagcatacg
    32701 ctcaccttcc agctgcacgg caatttccgc gccttcaggc agaccacggc agatatcaaa
    32761 gaatttgcgc gccggaacgg tcgtcgcccc tggttcgtgt ggctgaacca gcgcaacgcg
    32821 tgccaccatt tccatctcga gatcagtacc ggtcagcgac aacgtaccgt cagcaacctg
    32881 taacagcaga ttaccgagaa tcggtagcgt aggacgacca cctaacggcc cgctcacctg
    32941 ttgtagcggt tttaataaat gctcacgttc tacggtaaat ttcataggtt tacgatgaca
    33001 atgttctgat taaattggaa aaatcttctt tgatatcgtg gctctcttca cgcagctgct
    33061 cgatcttacg gcaggcatga agcaccgtcg tgtggtcacg gccaccaaac gcatcgccaa
    33121 tctccggcag actgtggtta gtcagctctt tcgccagcgc catcgccatc tggcgcggac
    33181 gcgccaccga gcgggatcga cgcttggaaa ggagatccgc gactttgatc ttgtagtact
    33241 ccgccaccgt cttctgaata ttgtcgatgg tgaccagttt ttcctgcaat gccagcaagt
    33301 cgcgcagcgc ctcacgcacg aagtcgatgg tgatcgcccg tccggtaaag ttggcgttag
    33361 caataacgcg gttcagcgcg ccttccagct cacgtacgtt agatcgtaga cgcttggcga
    33421 taaagaacgc cacttcgccc ggcaaacgaa tgtcgttttc gtcggccttt ttcatcagga
    33481 tcgccacgcg ggtttccagc tctggcggtt cgatcgccac agtcagcccc cagccgaagc
    33541 gggatttcaa acgatcctca acgccgttga tctctttcgg atagcgatcc gaggtgagaa
    33601 tgatctgttg attaccttcc agcagggcgt tgaaggtgtg gaaaaactct tcctgagatc
    33661 gttctttatt agcaaaaaac tgaatatcgt cgatcagcag tgcatctacg gaacggtagt
    33721 agcgtttaaa ctcttcgatc gcgttgtttt gcagggcttt aaccatgtcc tgaacaaagc
    33781 gctcggagtg catataaacc actttggcat tcggcttgcg cgccataatg ccgttaccca
    33841 ccgcatgcag caggtgagtt ttacccagac ccgtgccgcc ataaaggaac aacgggttat
    33901 aggcaccgcc agggttatct gccacctggc gagccgccgc gcgcgccagt tggttagatt
    33961 taccttcaac gaagttatca aacgtgtgtt tgacgtttac gttagaacga taggtcggtt
    34021 ctgccggggc cgggacgtta tcccaacctg agcgcgtaga aggcgcagca cgttgcggct
    34081 gcgtttgcgc cacctgtgca ggggccgcga cgttgctcgt cactgccgct tgtggcgttt
    34141 gcgtcaccgg tttggtgccg acttcaaaac gcagctgtgg ggcatccgct ccgcagaaac
    34201 tggttagcag tccattgata ttattaaggt acttgtcccg tacccaatcg aggacaaaac
    34261 gatttggcgc gtacagggcc agcgtgttat cgctcagttc cgcctgcaat gggcgtatcc
    34321 acatactgaa ttctgtggct ggtaactcat cctgcaatcg ggcaagacac tgctgccaaa
    34381 gcgaaagtga cacggcggac tccactcgaa caaaagtcga taatgactaa ggctgaaaca
    34441 ttcatgattg ttgacgtacg tcgaaaagac cctgcatgag ggtgacgcac gaaccgctgt
    34501 ctgcggttat atgcccgatc aagatcctgc aaaacgatcg ggaccgcgga tcatagccta
    34561 aactgcgcaa gagatcttct gtttctcaca gattttcccg atttatccac aggactttcc
    34621 agaactcgct aagtgtaaac gatcctgccg cgaggcgggc acgatttacg ccgcatattg
    34681 gaaaatttaa tgaccataga caaaaattgg cttaatcgat ctaataaaga tccaggacga
    34741 tccttgcgct ttacccgtca gcccgtataa tcctccaccc ggcgcgccat gctggttttc
    34801 actggtgtga ggtcgtacat tttccctgcg aaaaggtgcg gaaaagcgcg gtaaataagg
    34861 aaagagaatt gactccggag tgtacaatta ttacaatccg gcctctttaa tcacccatgg
    34921 cttcggtgtc catcgtttca tttttcggcg gatatccaat aaagccattg aatttattca
    34981 agtttaggta gaaatcgcca tgaaacgcac ttttcaaccg tctgtactga agcgcaaccg
    35041 ttctcacggc ttccgtgctc gtatggctac taaaaatggt cgtcaggttc tggcacgtcg
    35101 tcgtgctaaa ggccgcgctc gtctgaccgt ttctaagtaa taaagctaac ccctgagtgg
    35161 ttaagctcgc atttcccagg gagttacgct tgttaactcc cagtcaattc acattcgtct
    35221 tccagcagcc acaacgggct ggcacgccgc aaattaccat tctcggccgc ctgaattcgc
    35281 tggggcatcc ccgtatcggt cttacagtcg ccaagaaaaa cgttcgacgc gcccatgaac
    35341 gcaatcggat taaacgtctg acgcgtgaaa gcttccgtct gcgccaacat gaactcccgg
    35401 ctatggattt cgtggtggtg gcgaaaaaag gggttgccga cctcgataac cgtgctctct
    35461 cggaagcgtt ggaaaaatta tggcgccgcc actgtcgcct ggctcgcggg tcctgatagc
    35521 cctcattcgg gtctatcaac gcctgattag tccgctactc gggccgcatt gtcgtttcac
    35581 tccaacctgt tcaagctacg gaattgaggc attgcgcagg tttggagtga taaaaggcag
    35641 ttggttgacg gtgaaacgcg tattaaaatg ccacccttta caccctggtg gtgacgatcc
    35701 cgtcccgccc ggaccatttg ataccagaga acactaacga tggattcgca acgcaatctt
    35761 ttagtcatcg ctttgctgtt cgtgtctttc atgatctggc aagcctggga gcaggataaa
    35821 aacccgcaac ctcaggccca acagaccacg cagacaacga ccaccgcagc gggtagcgcc
    35881 gccgaccagg gcgtaccggc cagtggccag gggaaactga tctcggttaa gaccgacgtg
    35941 cttgatctga ccatcaacac ccgtggtggt gatgttgagc aagctctgct gcctgcttac
    36001 ccgaaagagc tgaactctac ccagccgttc cagctgctgg aaacttcacc gcagtttatt
    36061 tatcaggcac agagcggtct gaccggtcgt gatggcccgg ataatccggc taacggcccg
    36121 cgtccgctgt ataacgttga aaaagacgct tatgtgctgg ctgaaggtca aaacgaactg
    36181 caggtgccga tgacgtatac cgacgcggca ggcaacacgt ttaccaaaac gtttgtcctg
    36241 aaacgtggtg attacgctgt caacgtcaac tacaacgtgc agaacgctgg cgagaaaccg
    36301 ctggagatct ccacctttgg tcagttgaag caatccatca ctctgccacc gcatctcgat
    36361 accggaagca gcaacttcgc actgcacacc ttccgtggcg cggcgtactc cacgcctgac
    36421 gagaagtacg agaaatacaa gttcgatacc attgccgata acgaaaacct gaacatctct
    36481 tcgaaaggtg gttgggtggc aatgctgcaa cagtatttcg cgacggcgtg gatcccgcat
    36541 aacgacggta ccaataactt ctataccgct aatctgggta acggcatcgc cgctatcggc
    36601 tacaagtctc aaccggtact ggttcagcct ggtcagactg gcgcgatgaa cagcaccctg
    36661 tgggttggcc cggaaatcca ggacaaaatg gcagctgttg ctccgcacct ggatctgacc
    36721 gttgattacg gttggttgtg gttcatctct cagccgctgt tcaaactgct gaaatggatc
    36781 catagctttg tgggtaactg gggcttctcc attatcatca tcacctttat cgttcgtggc
    36841 atcatgtacc cgctgaccaa agcgcagtac acctccatgg cgaagatgcg tatgctgcag
    36901 ccgaagattc aggcaatgcg tgagcgtctg ggcgatgaca aacagcgtat cagtcaggaa
    36961 atgatggcgc tgtacaaagc tgagaaggtt aacccgctgg gcggctgctt cccgctgctg
    37021 atccagatgc caatcttcct ggcgttgtac tacatgctga tgggttccgt tgaattgcgt
    37081 caggcaccgt ttgcactgtg gatccacgac ctgtctgcac aggacccgta ctacatcctg
    37141 ccgatcctga tgggcgtaac gatgttcttc attcagaaga tgtcgccgac caccgtgacc
    37201 gacccgatgc agcagaagat catgaccttt atgccggtca tcttcaccgt gttcttcctg
    37261 tggttcccgt caggtctggt gctgtactat atcgtcagca acctggtaac cattattcag
    37321 cagcagctga tttaccgtgg tctggaaaaa cgtggcctgc atagccgcga gaagaaaaaa
    37381 tcctgattcg gtgagttttc gctaaaataa gggcggtcag ttgaccgcct tttttctttt
    37441 cgtagggcgg ataagcaccg cgcatccgcc acacaaagca acaggaacat catgagcgat
    37501 aatgacacta tcgtagccca ggccacgcct ccgggacgtg gcggcgttgg catcctgcgc
    37561 atctccggcc tcaaagcccg tgaagttgcc gaaaccgtgc tgggtaaact gcctaagccg
    37621 cgctacgccg attatcttcc gtttaaagac gccgacggca gcgtgctcga tcaggggatt
    37681 gcgctatggt tccctggccc gaactcgttc accggcgaag atgtgcttga actgcagggt
    37741 catggcggtc cggtgatcct cgacctgctg ttaaaacgca ttctgaccat tcccggcctg
    37801 cggattgccc gccctggtga gttttccgaa cgcgcgtttc ttaacgataa actcgactta
    37861 gcccaggctg aggcgattgc ggatcttatc gacgccagct cggaacaggc ggcccgttcg
    37921 gcgctaaact cgctgcaagg cgcattctcc gcacgggtca accatctggt ggaagccctc
    37981 acccacttgc gcatttacgt cgaagcggca attgatttcc ctgatgaaga gatcgatttc
    38041 ctctccgacg gcaaaattga agcccagctc aatgacgtaa ttgccgatct tgatgcagtg
    38101 cgtgctgaag cacgtcaggg tagtttgttg cgcgaaggga tgaaagtagt gattgctgga
    38161 cgtcctaacg ccggtaaatc gagcctgtta aacgcgctgg cgggccgtga agcggcaatc
    38221 gtaaccgata tcgccggaac cacgcgtgac gtgctgcgtg agcatatcca cattgacgga
    38281 atgccgctgc atatcatcga taccgccgga ctacgtgaag ccagtgacga agtagaacgt
    38341 attggtatcg agcgcgcgtg gcaggaaatt gaacaggccg accgcgtgct gtttatggtc
    38401 gatggcacca cgacagacgc cgttgatccg gcagagatct ggccggaatt tatcgcccgt
    38461 ctgcccgcga aactgccgat caccgtggtg cgcaataaag ccgatatcac cggcgaaacg
    38521 ctgggaatga gtgaagtgaa cggtcatgcg ttgattcgtc tctcggcaag gactggcgaa
    38581 ggcgtggacg tgctgcgtaa ccatctcaaa cagagcatgg gctttgacac caacatggaa
    38641 ggcggcttcc tggcgcgtcg tcgtcaccta caggcgctgg aacaagcggc ggagcatctg
    38701 caacagggca aagcacaact gttgggtgca tgggcaggtg aattgctggc ggaagagctg
    38761 cgcctggcgc agcagaactt aagcgagatc accggggagt ttacctctga cgacctgctg
    38821 gggcggattt tctccagctt ctgtattggt aagtaagtcg tctaaattga tataaagggc
    38881 actgttccgc tctgggtaaa ctaactatca gaacggaaca gcgcttgttt ttttatgagc
    38941 atcctgcacc aaaactgcat ttgctactgg ctggagcgac tttaaacacc ttgcagtcct
    39001 gtctttacag agaacgatat tgctttaacc tattaagctc ctcttgcgga aagttaaatt
    39061 cgtctactgc tttccagaat agttctttaa gtacaggatt gccatttgcc tgacttatta
    39121 cagatttacc ttcattattc ttttcttcca gctgcatcat cacatcttta aggtaatttt
    39181 cattttcacc aaccacaatt ttgaatattt tcagccagag tcccatatgt atatcatccc
    39241 cgttatttac cacatgagag aatggcttgc catcaaagtc acgggtaaac tgctccacaa
    39301 tttcatcgaa agtgaacccg tgttgcaacg ccaggcgaat cgccgcattc agatatacgt
    39361 caatgacctg tttactcatt ttcttaaagc ttgcaatatg caaagagcag acggcattat
    39421 ttctgctcag cgacagtagt tctctcacca catctttatc attttcataa tggctggcta
    39481 tcacatcagc tgtctccatc caggcggcaa caatctccgt ataccctttc actatcgcag
    39541 aatataagcc tgatgccttc gtccatttgt ctgactcccc cgcccattct cttgctgcca
    39601 tcagtttcaa tatatcttct ttatttattt catttttaaa catccgggct tttatattgg
    39661 cg
//
LOCUS       DACBVI010000024        39502 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.024, whole genome shotgun sequence.
ACCESSION   DACBVI010000024 DACBVI010000000
VERSION     DACBVI010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.024"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(85..224)
                     /locus_tag="IU325_06365"
                     /pseudo
     CDS             complement(85..224)
                     /locus_tag="IU325_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341766.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
     gene            complement(333..427)
                     /locus_tag="IU325_06370"
     tRNA            complement(333..427)
                     /locus_tag="IU325_06370"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(391..393),aa:Sec,seq:tca)
     gene            720..2102
                     /locus_tag="IU325_06375"
     CDS             720..2102
                     /locus_tag="IU325_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HAP5808665.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTIVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFLGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQNVQN"
     gene            2112..4430
                     /gene="yicI"
                     /locus_tag="IU325_06380"
     CDS             2112..4430
                     /gene="yicI"
                     /locus_tag="IU325_06380"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HAP5808666.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWK
                     VLKETVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTESG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDNTLLALGNNEQRPDYAW
                     HEGTAFQLFNLQDGHEAICEVPAADGSVLFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            4725..6278
                     /locus_tag="IU325_06385"
     CDS             4725..6278
                     /locus_tag="IU325_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP5808667.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVTIAGYLNVSEKTIQRDLRL
                     LEQWLEQWRITVEKRAGAGVMLDSKNITDLLHLDHLLGAECEEIDGVMNNARRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDMENEL
                     SWSLGEPYYVNIFTHILIMMYRNTHGNALSREEDQTRQYDENIFNVASQMIHKIEQRI
                     AHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSDIVDC
                     DFSEDSALYDGLLVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQVFKT
                     WGENAVSEDEVGYLTVHFQAAMERQIARKRVLLVCSTGIGTSHLLKSRILRAFPEWTI
                     VDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVITEKLH
                     HATSRVVEI"
     gene            6305..6775
                     /locus_tag="IU325_06390"
     CDS             6305..6775
                     /locus_tag="IU325_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004126743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP5808668.1"
                     /translation="MDITKILNTNRVILDMQATNKAEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIAQLLVETSPQKIIDLFSQYAQSDVY"
     gene            6793..7107
                     /locus_tag="IU325_06395"
     CDS             6793..7107
                     /locus_tag="IU325_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP5808669.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAAEISAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            7132..8223
                     /locus_tag="IU325_06400"
     CDS             7132..8223
                     /locus_tag="IU325_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009112672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP5808670.1"
                     /translation="MKENKIPVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRFLMGAILGAMATFDFGGPVNKTMSLFSDGLLVSGVYGPEAV
                     KFVGSIIPPFGITLSFLLTRHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVV
                     GSCVVASAVAGGLIMVWGVESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSLW
                     KKPVTERDEEFDELSDQKLKDEEITFTLE"
     gene            8235..9086
                     /locus_tag="IU325_06405"
     CDS             8235..9086
                     /locus_tag="IU325_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP5808671.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINCMNLESAHAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVPVALALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTRAVVELAASAQLCVEGELGHLADAPRYDQAANADLMTQPTDVEP
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLAEIFHCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGSIGKA"
     gene            9167..10027
                     /locus_tag="IU325_06410"
     CDS             9167..10027
                     /locus_tag="IU325_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP5808672.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPT
                     IIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLDHGASVEHVLRAIRCGFTSVMID
                     GSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
                     FIARTGADTLAVAIGTAHGIYPKGMQPKLQMDILRDIAGRIDIPLVLHGGSANPDAEI
                     AESVTLGVGKINISSDMKYAYFQKVREILAKESWWDPNVIYPDAINAAREVIRHKMKL
                     FGSLGKASLY"
     gene            10075..11007
                     /locus_tag="IU325_06415"
     CDS             10075..11007
                     /locus_tag="IU325_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP5808673.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VALIEQIRRDVQRPMLTGVALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYMEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQF
                     TTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            10988..11668
                     /locus_tag="IU325_06420"
     CDS             10988..11668
                     /locus_tag="IU325_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016155011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HAP5808674.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQRERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGAAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEANLCDDQRVITVSVDGQRRAVKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNHFLEPGGRFPAIEEDEPVKWLL
                     CSEYAVLR"
     gene            complement(11711..13420)
                     /locus_tag="IU325_06425"
     CDS             complement(11711..13420)
                     /locus_tag="IU325_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAP5808675.1"
                     /translation="MKFIGKLLLYIFIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWQRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANIPLKPTVSGQLHAVNATKQQVTQTMNAGVVSSGEVTSTE
                     PVQ"
     gene            complement(13541..14932)
                     /gene="xanP"
                     /locus_tag="IU325_06430"
     CDS             complement(13541..14932)
                     /gene="xanP"
                     /locus_tag="IU325_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HAP5808676.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            15212..16417
                     /gene="gltS"
                     /locus_tag="IU325_06435"
     CDS             15212..16417
                     /gene="gltS"
                     /locus_tag="IU325_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HAP5808677.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(16451..18532)
                     /gene="recG"
                     /locus_tag="IU325_06440"
     CDS             complement(16451..18532)
                     /gene="recG"
                     /locus_tag="IU325_06440"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP5808678.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDAPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRNDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18538..19227)
                     /gene="trmH"
                     /locus_tag="IU325_06445"
     CDS             complement(18538..19227)
                     /gene="trmH"
                     /locus_tag="IU325_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HAP5808679.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADRDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(19234..21342)
                     /gene="spoT"
                     /locus_tag="IU325_06450"
     CDS             complement(19234..21342)
                     /gene="spoT"
                     /locus_tag="IU325_06450"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP5808680.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(21361..21636)
                     /gene="rpoZ"
                     /locus_tag="IU325_06455"
     CDS             complement(21361..21636)
                     /gene="rpoZ"
                     /locus_tag="IU325_06455"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP5808681.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(21691..22314)
                     /locus_tag="IU325_06460"
     CDS             complement(21691..22314)
                     /locus_tag="IU325_06460"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP5808682.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            22572..24254
                     /gene="ligB"
                     /locus_tag="IU325_06465"
     CDS             22572..24254
                     /gene="ligB"
                     /locus_tag="IU325_06465"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HAP5808683.1"
                     /translation="MKVWMAILISILCWQSSAWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGIYDQLSARLTQWQRCFGNETRDVMMPPLNGAVIHPVAHTGVRK
                     MADKIALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVRL
                     ISAVPQTASGPLANSTLQGEIFLKREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELTTAGFTLTQTYTRAVKNADEVARVRNAWWKAKLPFVT
                     DGVVVRAAKEPESRYWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRVALNASDERS
                     WSQLLLSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(24251..24868)
                     /locus_tag="IU325_06470"
     CDS             complement(24251..24868)
                     /locus_tag="IU325_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HAP5808684.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(25159..25983)
                     /gene="dinD"
                     /locus_tag="IU325_06475"
     CDS             complement(25159..25983)
                     /gene="dinD"
                     /locus_tag="IU325_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HAP5808685.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACLNSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQISIKQLENSVKITEKK"
     gene            complement(26204..27067)
                     /locus_tag="IU325_06480"
     CDS             complement(26204..27067)
                     /locus_tag="IU325_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP5808686.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            27194..27910
                     /locus_tag="IU325_06485"
     CDS             27194..27910
                     /locus_tag="IU325_06485"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP5808687.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            27976..28617
                     /gene="pyrE"
                     /locus_tag="IU325_06490"
     CDS             27976..28617
                     /gene="pyrE"
                     /locus_tag="IU325_06490"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP5808688.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(28654..29250)
                     /gene="slmA"
                     /locus_tag="IU325_06495"
     CDS             complement(28654..29250)
                     /gene="slmA"
                     /locus_tag="IU325_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HAP5808689.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(29357..29815)
                     /locus_tag="IU325_06500"
     CDS             complement(29357..29815)
                     /locus_tag="IU325_06500"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP5808690.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(29793..31013)
                     /gene="coaBC"
                     /locus_tag="IU325_06505"
     CDS             complement(29793..31013)
                     /gene="coaBC"
                     /locus_tag="IU325_06505"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HAP5808691.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRERGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            31185..31853
                     /gene="radC"
                     /locus_tag="IU325_06510"
     CDS             31185..31853
                     /gene="radC"
                     /locus_tag="IU325_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAP5808692.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            32070..32306
                     /gene="rpmB"
                     /locus_tag="IU325_06515"
     CDS             32070..32306
                     /gene="rpmB"
                     /locus_tag="IU325_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP5808693.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            32327..32494
                     /gene="rpmG"
                     /locus_tag="IU325_06520"
     CDS             32327..32494
                     /gene="rpmG"
                     /locus_tag="IU325_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP5808694.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            32592..33401
                     /gene="mutM"
                     /locus_tag="IU325_06525"
     CDS             32592..33401
                     /gene="mutM"
                     /locus_tag="IU325_06525"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HAP5808695.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCEKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(33440..33919)
                     /gene="coaD"
                     /locus_tag="IU325_06530"
     CDS             complement(33440..33919)
                     /gene="coaD"
                     /locus_tag="IU325_06530"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP5808696.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(33927..35204)
                     /locus_tag="IU325_06535"
     CDS             complement(33927..35204)
                     /locus_tag="IU325_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HAP5808697.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            35617..36675
                     /gene="rfaQ"
                     /locus_tag="IU325_06540"
     CDS             35617..36675
                     /gene="rfaQ"
                     /locus_tag="IU325_06540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HAP5808698.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLVCVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            36672..37796
                     /locus_tag="IU325_06545"
     CDS             36672..37796
                     /locus_tag="IU325_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP5808699.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEVE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            37789..38586
                     /gene="rfaP"
                     /locus_tag="IU325_06550"
     CDS             37789..38586
                     /gene="rfaP"
                     /locus_tag="IU325_06550"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HAP5808700.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDIIKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            38602..>39502
                     /gene="waaO"
                     /locus_tag="IU325_06555"
                     /gene_synonym="rfaI"
     CDS             38602..>39502
                     /gene="waaO"
                     /locus_tag="IU325_06555"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HAP5808701.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVSYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDAIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRLTY
                     MDQDILNLILLGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFIKAKEASPWKNEPLM"
BASE COUNT         9460 a         9592 c        10519 g         9931 t
ORIGIN      
        1 gtcagttttt ttcgccagct gttaaaggaa tagaatcgtc gtataaaaaa ggtgttctgg
       61 ggcgtatgaa acaacatctt caccttaatg gcgatacagt tggctgagag ccagcagggg
      121 gtaaatcacc tgcaaataaa cacggcacat gctggcgcgc atatcgcgga tgcgttgagg
      181 acagaagaac cgacaggcgg attcttcagc cgctcatcgt ccatcacaaa acctttgacc
      241 aggtattcct gaagccgatt gaatattgag cagtagaaaa tatctggatt gacagtatta
      301 caagggattg gagaacgtca gaagaggtat tttggcggaa gatcacagga gtcgaacctg
      361 cccgggaccg ctggcggccc caactggatt tgaagtccag ccgcctcacc ggagacgacg
      421 atcttccgcg cctcgattgc tacatggagg cggggcgcat tatagctact tccttgagtt
      481 tctacatccc ccagatcagt tttttccgcc taatacacgc agcgtattat cacggaagca
      541 ttataattca tataaaaccc atattttaca atgatatatg agcggaatta acgctaccac
      601 gatcacataa aacaagaatc ataaattaat aaccagatat cggaatattc gctctcacag
      661 ggatggttta caaaatgcgt tatcggtctc agcaccccta tagcatcaag gaaaagcaga
      721 tgaagagtga agtgttgtcc gttaaagaga aaattggtta tggcatggga gacgccgcca
      781 gccacattat tttcgataac gtaatgttat atatgatgtt cttttatacc gatatttttg
      841 gcattcctgc cggatttgtc ggaactatgt ttttggtcgc tcgtgcactg gatgcgattt
      901 ccgatccttg catggggttg ttggccgatc gaacgcgctc tcgctggggt aaatttcgtc
      961 cgtgggtact gtttggcgca ctgccatttg ggatcgtctg tgtactggcc tatagcacgc
     1021 cagatctcag tatgaacgga aaaatgattt atgcagcaat tacttacacc ctacttacct
     1081 tactttatac catcgttaat atcccttact gcgcattggg tggtgtaatc accaatgacc
     1141 cgactcagcg tatctcgctg caatcctggc gttttgtgct ggcgaccgcg ggaggcatgc
     1201 tctctactgt gctgatgatg ccgctggtta atttaattgg tggtgataat aaaccactcg
     1261 gtttcctggg cggtatcgcg gtcctttccg tggtggcatt catgatgctg gcattttgtt
     1321 tctttaccac taaagaacgc gttgaagcac cacctacaac gacgtctatg cgggaagatt
     1381 tacgtgatat ctggcaaaac gatcagtggc ggattgtcgg tttactgacc attttcaata
     1441 ttctggcggt gtgcgtacgc ggcggggcga tgatgtatta cgtcacatgg attttgggta
     1501 cgccggaagt gtttgtcgct tttcttacca cttattgcgt gggtaacctg attggttccg
     1561 cactggcgaa accgctgacc gactggaaat gtaaagtcac tatcttctgg tggacgaacg
     1621 ccctgctggc agtgattagc ctcgcgatgt tctttgttcc tatgcaggcc agcatcacta
     1681 tgtttgtctt catcttcgtg attggtgtgt tgcatcaact ggtgacacct atccagtggg
     1741 taatgatgtc cgataccgtc gactacggcg agtggtgcaa tggtaaacgc ctgaccggga
     1801 tcagttttgc tggcacgctg tttgtgctca agctggggtt ggccttcggc ggcgctctta
     1861 tcggctggat gctggcttat ggcggatatg atgcggcaga aaaagcgcag aacagcgcca
     1921 cgattagcat cattatcgcg ctattcacga ttgttccggc gatctgttat ttgctgagcg
     1981 cgattatcgc taaacgctac tactcactca cgacgcacaa tctgaaaacc gttatggaac
     2041 agctggctca gggcaaacgc cgttgccagc aacaattcac ctctcaaaat gtgcagaact
     2101 aaggaacggc aatgaaaatt agcgacggaa actggttgat tcaacctggc ctcaatttga
     2161 ttcacccgct tcaggtgttc gaggttgaac agcagggtaa tgaaatggtg gtctatgctg
     2221 cccctcgtga tgtgcgtgaa cgtacctggc agcttgatac gcctttattt acgctgcgct
     2281 ttttctcccc acaggaaggt attgtcggtg tacggattga gcattttcag ggggcgctga
     2341 ataacggtcc tcattacccg ctcaatattt tgcaggacgt gaaggtcaca atcgaaaaca
     2401 cagaacgtta cgctgagttt aaaagtggca acttaagtgc gcgtgtcagc aaaggtgagt
     2461 tctggtcact ggattttctg cgtaacggcg aacgtattac cggtagtcag gtgaaaaata
     2521 atggctacgt gcaggacacg aataatcaac gaaattatat gtttgagcgg ctggatcttg
     2581 gcgttggcga aacagtttac ggtctgggag agcgctttac tgcgctggtg cgcaatggcc
     2641 agacggtaga gacctggaac cgggacggcg gcacaagtac tgaacaggcg tataaaaata
     2701 tcccgttcta tatgactaac cgtggctatg gggtactggt caatcatcct caatgcgtct
     2761 cttttgaagt gggatcggag aaagtctcca aagtgcagtt cagcgttgag agtgaatatc
     2821 tcgaatactt tgttatcgac ggcccgacgc cgaaagcggt acttgatcgt tatacccgtt
     2881 ttactggtcg tccggcgctg ccgcccgcgt ggtccttcgg cctgtggcta accacttcat
     2941 ttaccaccaa ctacgacgaa gcgacggtaa acagttttat cgatggtatg gcggaacgca
     3001 atctgccact gcatgttttc cactttgact gtttctggat gaaagccttc cagtggtgcg
     3061 attttgagtg ggacccgctg actttcccgg acccggaagg gatgatccgc cgcctgaaag
     3121 cgaaagggct aaaaatctgc gtctggatta acccctatat cgggcaaaaa tcccctgtct
     3181 ttaaagagtt acaagagaaa ggctatttac tcaaacgccc ggacggttcg ttatggcagt
     3241 gggataaatg gcagccaggt ctggcgattt atgactttac caatccggat gcctgcaaat
     3301 ggtacgccga caaactgaaa ggtctggtcg cgatgggcgt tgattgcttt aagaccgact
     3361 ttggcgaacg tattccaacc gatgttcagt ggtttgacgg ttccgatccg cagaaaatgc
     3421 ataaccatta tgcgtacatc tacaacgaac tggtgtggaa agtgctcaag gaaaccgttg
     3481 gcgaggaaga agccgtcttg tttgctcgct cggcctccgt tggtgcgcag aaattcccgg
     3541 tacactgggg tggtgactgt tacgctaact acgaatcaat ggcggaaagc ctgcgcggtg
     3601 gtttgtctat tggcctttca ggttttggtt tctggagcca cgatatcggc ggctttgaga
     3661 ataccgcgcc ggcgcacgtt tacaaacgct ggtgcgcgtt tggtttgctc tccagccata
     3721 gccgtttaca cggcagcaaa tcttatcgtg tgccgtgggc ctacgatgat gagtcctgtg
     3781 atgtggtgcg cttcttcacg caactgaaat gccgcatgat gccgtatctg tatcgtgaag
     3841 ctgctcgtgc gaacgcgcgg ggtacgccga tgatgcgggc catgatgatg gagtttccgg
     3901 acgatccggc ttgtgattac cttgaccgtc aatacatgtt aggcgacaac gtgatggttg
     3961 cgcctgtgtt cactgaatcg ggcgatgtgc agttctactt gcctgaaggt cgctggacac
     4021 acctgtggca caacgatgaa ctcgatggta gtcgctggca taaacagcag cacagcttcc
     4081 tgagtctgcc cgtttatgtg cgtgataaca ccctactggc gctgggcaac aacgagcaac
     4141 gtcccgatta cgcgtggcac gaaggcacgg cattccagct ctttaatcta caagacgggc
     4201 atgaagccat ctgtgaagtg cccgctgctg acggttccgt tcttttcacc ctgaaagcgg
     4261 cgcgtactgg caacacgatt actgtgactg gtgcgggcga ggcgaagaac tggacactgt
     4321 gcttgcgcaa tattgtgaaa gtaaatggtc tgcaaggcgg ttcgcaggct gaaagtgagc
     4381 agggactggt ggtgactcct caagggaatg cgctgacaat tacgttgtaa tctattgaag
     4441 aatgccggat acgaatggtt ttgtgtcgta tccggtaaat aatctcacat ctggtaataa
     4501 atatttcttc catcgtattt gcgagcatga tcacgattat tcatcacgaa aatgtgatct
     4561 gtgccgaaaa atatctatta accgccccgt tttgttttat acatgtccgg actacggaca
     4621 gattttagta ttgttgtgaa attattctgc tttatattta ccctcagaga tggcaactga
     4681 agctcttcca catggggctt cttaatctgt gataagggta aaaaatgcaa atgattacct
     4741 cgcgacaaaa cagattatta cgattccttt taccacgaag ggaatatacg actattgtta
     4801 cgattgccgg ctatttaaat gtttcggaaa aaaccattca acgtgattta cgtttacttg
     4861 agcaatggct ggaacaatgg cgaataaccg ttgagaagcg tgctggcgcg ggtgtgatgt
     4921 tagactcgaa gaatattact gatttgctgc atcttgatca tttgttggga gcagaatgtg
     4981 aagagattga tggtgtaatg aataatgcca ggcgcgttaa aatagcgtcg cagttattaa
     5041 gtgaaacacc gaatgaaacg tcgatcagta aattgtcaga gcgctatttt atcagtggag
     5101 cctctattgt taatgacctg agagtgattg agtcctggct tgcgccgttg gggttatcat
     5161 tgatccgcag cccaagtggt acgcatattg aaggtagtga agggcaagtc cgacaggcaa
     5221 tggcattact gattaacggc attattaacc ataatgagcc gcaaggtgtc gtgtattcac
     5281 gtctggatcc cggaagttat aaagcattag tccattattt tggagaagaa gaggtgttat
     5341 ttgtccagtc attgttactg gatatggaaa atgaattaag ttggtctttg ggagagcctt
     5401 attacgttaa catttttact cacatcctta ttatgatgta tcgcaacacg cacgggaatg
     5461 cgttatccag agaagaagat caaaccaggc aatatgatga aaatatcttt aatgttgcca
     5521 gtcagatgat tcataagata gaacaacgaa ttgcacatac attgcccgat gatgaagtct
     5581 ggtttattta tcaatatatc atttcatcag gtgtggcgat tgatggacaa aaagatgtga
     5641 gcattatttc acatatgcag gccagcaatg aagcgcgtct gattacctgg cgtttaatta
     5701 cggtattcag tgacatcgtg gactgcgatt ttagtgaaga cagcgcatta tatgatggct
     5761 tgttggttca tattaagccg ctgattaacc gactaaatta tcgcattcat atccgtaatc
     5821 cattgctgga agatattaaa gcagaattag cggatgtctg gcggttgacg caatatgtgg
     5881 tgaatcaggt atttaaaacc tggggtgaga atgcagtgag cgaggatgaa gtaggttacc
     5941 tgaccgttca ttttcaggct gcgatggagc ggcaaattgc ccgtaaacgt gtattactgg
     6001 tctgttcaac cggaatcgga acttcgcatc tactgaaaag ccgtattctg cgagcatttc
     6061 ctgaatggac gattgttgat gttatttcag cagcgaattt atcacaggtt ttgcctgaca
     6121 atatcgaact gattatttcg accattaatt tgcctacagt cactatgccg gtcgcttatg
     6181 ttaccgcttt ttttaatgat gccgatatta agcgggtcac tgaaatggtg attacggaaa
     6241 aattacatca tgcgacatct cgggtcgttg aaatttaatg tcagaatttt atgaggtatg
     6301 acgaatggac ataacaaaaa tcctgaatac gaatcgcgtt attttagata tgcaagcgac
     6361 gaataaagcg gaggcgattg aagaattaac taatcttctc aaaaaagatg gtgcgatcga
     6421 ttgtcgggaa acctttatta aagatgtctg gcagcgcgaa gcggaaggct cgactggttt
     6481 tgaaaatcat attgcgatcc cgcatggtaa atcttctgcg gtaattaata ccacgctggc
     6541 tatcgggcgt acccggcagg atattccctg ggaaacactg gatggcagta gcgtacgctg
     6601 tattatttta tttgcggtac gtcttgaaga tcaaaatacc actcatatcc gtttactttc
     6661 ccaggtcgcg ggcgcgcttg cagatgatga catcatagcg caattgctgg ttgagacgag
     6721 cccgcaaaaa atcattgatc tctttagtca gtatgcacaa tccgatgtct actaaataac
     6781 agggagtata tcatgaatat tgtctgtgtt gccgcctgca cggcgggtat tgctcacacc
     6841 tatattgctc gtgaaaagtt aattaaaggg gcaaaagcac tgggccacac cattaaagtt
     6901 gaaacccagg gaaccattgg tacagaaaac gaattacagg cggcagagat ctctgctgct
     6961 gacgtcgtga ttcttgctgt ggatgtgaaa ataaaaggag aagaacgttt taccaataaa
     7021 cgcattgtgc gagtaaagac agagatcgtc attaaatcgc ccgttcaatt tcttgaaaag
     7081 gtcgaaaaat cactgggaaa taaatgatcc ttatactgga gggatcttaa tatgaaagaa
     7141 aataaaattc cggtttcaca ggaaattaaa aaacatttat taaccggcat ctcgtggatg
     7201 atacccctta ttgtggccgc cgggatatgt attgcgttgg ggcaagttct tggcggtaca
     7261 gatgttggtg aaaaaacagg aacaatcccg tggatgctta atcagattgg cggctgggga
     7321 atggggctga ttgtgccgct gattagcgcg gcaattgcct attctattgc cgatcgtccc
     7381 ggatttgccc cgggcctgat tgtcggcttt ctctgtgggc aaatccatac cggatttatt
     7441 ggcggtatgt tgggtggttt cctggtgggc tacaccattt tgctacttaa acgctatatt
     7501 cgcctgccgc agtcgatgca aggactcatg ccaatcatgg tgttaccggt attgagcacc
     7561 attattggtg gtctgttaat gatgacgctg attggtaaac ccatcgcctg gttacaagaa
     7621 gcactgattc atttgctcga gtcgatgcag ggcggatcgc gcttcctgat gggggccata
     7681 cttggcgcga tggcaacctt cgatttcggt ggtccggtca acaaaaccat gtcgctgttt
     7741 tccgatggct tgctggtcag tggcgtctac ggaccagaag ccgtgaaatt tgtcggctca
     7801 atcatccctc cgtttggcat cacactttcg ttcttactga cacgccataa atacacccgt
     7861 gcggagcgcg aggcgctgaa agccgcgttt ccgatgggga tctgcatgat cactgaagga
     7921 gtcattccga tcgccgcgcg tgacctgttg cgagtagtgg gatcttgtgt ggtggcatcg
     7981 gcggtcgcgg gcggcttaat catggtgtgg ggcgtggaga gtccggttcc acatggcggc
     8041 atgtttgttg tgcctctgtt cacccatcct ctgctgtttt gcctggcgct gggtataggc
     8101 acggtgattt gtggcgtgat gctttcactc tggaagaaac cggtaacgga acgcgacgaa
     8161 gagtttgacg aactgagcga tcaaaagctg aaagatgaag agatcacttt caccctcgaa
     8221 taaccggagg ccctatgttt gccgatatga aaagtatggt gatgaaagcc tggcatgaac
     8281 attacgcctt gctggcgatc aactgtatga atctggaaag tgctcacgca gctatccgcg
     8341 tggcggagaa gaatcgcgct ccgatcattc tcaatctcta tcaggggcat ctggcgcatt
     8401 ttcctgcacc cgtcgcggcg gcagtggtta aaacgctcgc ggaatctgcc tcagtaccgg
     8461 tcgctctggc gcttgatcat ggcaaaaatc cggactgtat tcgtcaggcg tttcgtgctg
     8521 gtttcagcgg attaatgatt gatgcttctg cttttcccct ggaggaaaac gtgcgccaga
     8581 ccagggcggt ggtggaactc gccgccagcg cgcagctctg cgtggaaggc gaactgggcc
     8641 atctggcaga tgcgccgcgt tacgaccagg ccgccaatgc agacctgatg acgcaaccca
     8701 ctgatgtgga accgtttatc gcgcagacag gcattgatct actggccgtc tcggtaggca
     8761 cggcgcacgg catgtacgcg cctggtgttg tacctgctat tgattttcag cgtctggcgg
     8821 agattttcca ctgctcgagc gtgcctctgg cattacatgg cggtagcggt acgccgttcg
     8881 atcagctcca gctctgtacg acgctggggg tagcaaaaat caacgtgggg gcggccattt
     8941 ttgaacgggg gaaatccgcg ctcctgcata ccctctgtca cgatatatcc attgaactgg
     9001 tggacgcgct gaaagctatg gaactggcct tcgaagaagc gattacaccg tatttacagg
     9061 cgagcggctc tataggcaaa gcctgattca ttttctgtca tggcctttat gccagtgcaa
     9121 taacgcaccg gcagggcgga ctacacgctg aaactggaga tttaacatgt tagtttcgat
     9181 gaaagaatta ctgcaaccta cccgccagca cggctttgcc attggcgcat ttaatgtggc
     9241 ggataactgt ttccttcgcg cagtcgtgga tgaagcagaa gcgacaaata ctccgacgat
     9301 tattgccatc catcccagcg aacatgattt tgtgggcgat gcctttttcg ctcacgttcg
     9361 cgaaattacc cagcgtagtc cggtcccgtt tgtactgcac ctcgatcatg gtgcctctgt
     9421 agagcatgtt ttgcgtgcga ttcgctgcgg atttacctca gtaatgatcg acggttcgct
     9481 gctgccgtat gaagaaaacg tggcgctgac cgcagaagtg gttcgcctcg ctcacgcggt
     9541 tggagtgtcg gttgagggtg aactgggaac catcggacaa acaggaacgt cggtagaagg
     9601 tggtgtttca caagtgacct acacagatcc ggcgcaggcc gccgatttta ttgctcgtac
     9661 aggtgccgac acactggctg tggcgattgg tacagcgcat ggcatctatc caaaagggat
     9721 gcagccgaag ctgcaaatgg acattttgcg cgacattgcc gggcgcattg atattccatt
     9781 ggtgttgcac ggtggttcgg caaatccgga cgcggagatt gctgaatcag ttacgctcgg
     9841 cgttggcaaa atcaatatct ccagcgatat gaaatacgcc tatttccaga aagtgcgtga
     9901 aattctcgcc aaagagagct ggtgggatcc gaacgtcatc tatccggatg cgattaacgc
     9961 ggcgcgggaa gtcattcgcc acaaaatgaa actgttcggt tcgctcggta aagcatcact
    10021 gtactaagcc agaagggcgg gatgacccgc cttttacgca acaaggaacg agatatgcac
    10081 tatctggggt tggatattgg tgggaccaaa atcgccgccg tcgtcatgga tgcgcatggc
    10141 tgggagattc gccgttaccg ctgcccgacg caaaagtcga catatcaaca atttgtctca
    10201 tgcgttgtgg cgcttatcga gcagattagg cgggacgttc aacgaccgat gctgacaggg
    10261 gtcgccttac ccggcagtat ctcgccactc actggcctga ttaaaaacgc gaatattcag
    10321 gtgattaacg gtcatgcgtt acaggctgat ttgcagcaat tgcttgggca accggtggtg
    10381 atagccaatg atggtaactg tttcgcgcta tcagaagctt gcgacggtgc cgggcaagat
    10441 tatgacgtag tatttggtat tacgcttggt acaggctgcg gcggtggcat tgccatcaag
    10501 caacgaccgt ttataggggc ctggggaaat gctgccgaat gcggtcatat cacgttgcca
    10561 ggctatatgg agcaggaaga tggtccatca gtcagttgct attgcggcaa acacaactgc
    10621 gtggagtcgt ttgtttccgg cagcggtttt agtgaacgct atcaacagat gactggtaac
    10681 ttgctcactc ctgcggcgat tgtcaccctg gcacaacgtg gtgatgcttg tgccatgcag
    10741 caggtggcac gttttcgcca acagcttgcc cgcacgctgg caaccatcgt taacgttgtt
    10801 gaccctggcg tgattgtcat cggcggcggg ctttcgaatg tggaactgct tatcaccgat
    10861 ctgaacgcag aagtcgctcc actggttttc accgatcaat tcaccactcc cattgtaaaa
    10921 gcacagcacg gcgacagtag cggaatgcgt ggcgctgcct ggcttgctat gcgcaacgga
    10981 gaagccaatg aaacgttcac aaattaatta tgttattgat aaagcacatg ccattgcgca
    11041 gaccttccgc gtttgtttgc cagagttcgc ctattttacg gtggatgact ggctgcagcg
    11101 ggaacgggat aactggcaag aagtggtcga cttacagctc ggttgggata tcaccgattt
    11161 tggtcgtggt gatttcaacc aaacgggact aacactgcta acgatgcgca acggcgcgct
    11221 gggggcggcg gcatatccaa aaccgtatgc tgagaaaatg ttgcagatcc aacaagatca
    11281 gcagactccc tggcattttc acacccacaa aatggaagat atcctcaatc ggggcggtgg
    11341 agacttatgc atgcagttgg cctgggccaa cgaagccaat ctctgtgatg atcagcgggt
    11401 tattaccgtg agtgttgatg gtcagcggcg tgccgtgaaa cctggtgaaa cgctggtgct
    11461 aaagccgggg cagggcattt gcctgccgcc gcgtctgtat catcgcttct gggcggaaaa
    11521 agcgtttgtt ctcggctggg aaatttcgat ggtaaatgat gaccaacatg ataaccattt
    11581 tcttgagccg ggaggacgtt ttccggcgat agaagaagat gaaccggtga aatggttatt
    11641 gtgtagtgag tatgctgttt tgcggtaata gcggggacgc taacgttgat atccgttagc
    11701 gcccgcgtat ttactgcacc ggctccgtcg atgtaacttc gccactggaa acgacgcctg
    11761 cattcatggt ttgagtcact tgctgctttg tggcgttcac ggcatgcaat tgcccgctaa
    11821 ccgtaggttt cagcgggata ttggcctgaa tatcgccgct ggcggtaagc tgaatattgc
    11881 cgtcgccagt caacggtaat tcaggccatc cccactgttg caaaatattc accggcacac
    11941 cgcgtccatt caggctgata tgggtctgac gttgtggcgt ttgtgaaaca ctggcggtgg
    12001 cttccagaat gcctttttca gtaaatgcac tcagttcgct gatattcacc gtgctgctgt
    12061 tggcggtcag cgccagcgat gggcgacgaa catcaacacg attaaatgtc gcggcggcgg
    12121 cattcagatt cgccgaacca ctccagacgc cccatttatg atcggtaacc agcgtcaggt
    12181 tggcaccgta accatcgagc gcggtgagct gccacgggaa gtcagggtcg atatcaatga
    12241 tcagattgcg gctggcgcta aatctcttca gttgcaggct gtttaaccaa ccgggtgttg
    12301 tttccatcca caactgttgc cagttttttg gcaaggtata ttccagtccg gcaattgccg
    12361 catcatcgag gatcagcgtt ttcccgtcgc gttgccagtt tcctgacgtt ctgaccatac
    12421 ccccttccca gcggctggtg aactggcgca gcgctacgcc ctgcggggaa aattccgcgt
    12481 ttataatcgg gtcaaataaa tgcagcgaac cataaatgaa ctcgctggcg ttcatcgaca
    12541 gtttgccttc ctgtgtctgc cagtcatctt tactgaaggt catgttgcgc aagctgagat
    12601 cgaggtcggt caccgcccag tccggacctt gcaaacgagc atcgatcact tccaggcgac
    12661 caatctgcaa cgacgggaca gagcgtaatg gcgcaaagaa gtcggttagc gatttttcgc
    12721 tttgtagacg gatatccgcc atgcgcagat tttccacttg ccagctgccg tcggcgttac
    12781 gctgcgcgtt tccggttaat gtcccgcggg cgatgtcggc acccaggtta gtcagcgtaa
    12841 cgcgatcgtt atcaatactg ccttcaatca gtacattggt ggcaggaaca tcattgagcg
    12901 aaagcgatcc ggcactaaac tgaatctgcg ccttcgtacc cagcacttta ccggcttctg
    12961 gtgaccacgg aaccacgccg ccatttaccc gctgcgcgct cagtttccat tcgctattcg
    13021 ggctattaaa cgccatatca cgcagttgca gacgatcggc tttgaacggt agcggcgcgg
    13081 tctggtcggt gagattcagc gtcccgtttt ccagcaggat ggtatcgaca tggcgtggtt
    13141 cggtcagttg ccgactgctt agcgcaatgt cgacactttt tgccaccagg gtcgcgggct
    13201 ggccatcacg gccgaacgtg acgttctcca gcacgatatg agatggcgcg gaaaaacggt
    13261 gatccatcgc cccgaaggcc agatgatagt cgctattctc ggaaacccat gcgctgatat
    13321 gttctgctcc ccagcgggtt tgcagaagaa aatagaggcc agcgatcacc actaacagag
    13381 cgatgaaaat gtagagaagc agcttcccaa taaatttcat ggtcttccat cccgtgaaat
    13441 gcacataagg gagttatgca cgatttacgc gcaatcctca aggcgggaat ggtgaaagag
    13501 gtgagactgc ggcggtaatt atcttaccgc cgccagtgaa ttactgtttt tctggtggga
    13561 aaatcagatt cagcacgatg gcagtaatac cgcccgcggc gatcccggag gagagcaggt
    13621 ttttcagcca ctcaggggca aactgcaaaa tcagcggctg ctgagagacg cccagaccaa
    13681 ccgccagcga cagcgcgata atcagaatcg cccgacggtt cagcggctca cgagacacaa
    13741 tgcgcacacc ggaggcggcg atggtgccaa acattacaag tgttgcgccg cccagaaccg
    13801 gttctggaat atgttgtaca aaaccgctca ctgccgggaa cagacccagc acgatcaaca
    13861 tcagcgcgac gacaaaaccg acatagcggc tggcaacccc ggtcaactgg atcactccgt
    13921 tgttctgccc gaagcaggag ttcgggaagg tgttaaacac cgccgaaaca aacgagttca
    13981 ggccgtttgc cagcacgccg cctttcaggc gtttcatgta cagtggaccg gacactggct
    14041 gttcggaaac gtcagaggtc gccgtgatat cgccaatggt ttccagtgaa gtgatcataa
    14101 agaccagcat cagcggcagc agcagactcc attcaatgcc aagaccgtaa tagagcggcg
    14161 ttggcaccat aatcagttct tgcgtcatcg gttcgttgct ttctggcaac atgcccataa
    14221 accacgccag cgcatatccg gccgccatcg caatcaccag tgaggccacg cgtaagtaag
    14281 ggttacgttg acggttaagc aggataatta aggctaagac cacgcctgcc agcagcagat
    14341 ttttcggtgc gccgaaggtg ttatcgctca tggctgcgta accgccgcca atggaagtca
    14401 gcccaacctg aattagcgac aggccgataa tcatcaccac aacgccagaa accagcggcg
    14461 taataatgcg gcgcgccaga tgcagaacgc gggagatcac catctcggtg caacttgcca
    14521 gcatcaacgt gccgaacaaa gccgccatca tggtaggaac atcagcacca ccggtcttca
    14581 gcgctgtgcc gcccataatc agcggggcaa caaagttgaa gctggtgccc tgaatagaca
    14641 acaacccgga gccaaccgga ccccaggcct taatttgaat aatcgatgcc acaccggagg
    14701 caaacagcga catactaata atgtgttgcg tgtcttgtgc cggtaaaccc agcgcctggc
    14761 agattaatag cgctggcgtg atcaccgcaa cgaacatcgc cagcagatgc tgacaggcgg
    14821 caaacagagt ttgaggaagc ggcggacgat cttcaagacg gtaaatcagt tcgctgtttt
    14881 gagtctgcgc aaccggttgc gcattttctg actcgagggt ggaaacagac atcgaaaaca
    14941 atcccgtggt agcaaagcgg gcattttagc tgaccgaaag agaaaagcaa acgtttgcca
    15001 catcctcatc taaatattta aatgagataa atgcactttt ttatataact tttggtattt
    15061 ttctgcttaa aatccatgcc atttgttcat taacagctga gaatttgacc caggaaagca
    15121 taactcgata cccgtcatag ttcatgaagt tgccactgcg cgatgaagta tgacgagtat
    15181 gaaagagtga tgcggataca aaggagtaac tatgtttcat ctcgatactt tagcaacgct
    15241 tgttgccgca acgctgacgt tgctgctcgg gcgtaagttg gtccattccg tctccttttt
    15301 gaagaaatac accataccgg aacctgttgc gggtggtttg ttggtggcgc tggcgctact
    15361 agtactgaaa aaaagcatgg gctgggaagt caactttgat atgtccctgc gcgatccgtt
    15421 aatgctggct ttcttcgcca ccattggcct gaacgccaac attgccagtt tgcgtgccgg
    15481 tgggcgtgtg gttggcatct tcttgattgt ggttgttggt ctgttggtga tgcaaaatgc
    15541 cattggcatt ggtatggcta gcctgttagg gcttgatccg ctgatggggc tgttggccgg
    15601 ttctattacg ctttccggcg gtcacggtac gggcgctgcg tggagtaaat tgttcattga
    15661 acgttatggc ttcaccaatg cgacggaagt ggcgatggcc tgtgcaacgt tcggtctggt
    15721 gctgggcggc ttgattggcg gtccggtggc gcgctatctg gtgaaacact ccaccacgcc
    15781 gaacggtatt ccggatgacc aggaagtccc gacggcgttt gaaaagccgg atgtgggacg
    15841 catgatcacc tcgttggtgc tgattgaaac tatcgcgctg attgctatct gcctgacggt
    15901 ggggaaaatt gttgcgcaac ttttggctgg cactgctttt gaactgccga ccttcgtctg
    15961 tgtcctgttt gttggcgtga ttctgagcaa cggtctgtca atgatgggct tttaccgcgt
    16021 ctttgagcgt gcggtatccg tgctgggtaa cgtaagcctg tcgttgttcc tggcgatggc
    16081 gctgatgggg ctgaaactgt gggagctggc ttcgctggcg ctgccgatgc tggcgattct
    16141 ggtggtacag accatcttca tggcgttgta tgccatcttc gttacctggc gcatgatggg
    16201 caaaaactac gatgcggcag tgctggctgc gggtcattgt ggttttggcc tcggtgcaac
    16261 gccaacggca atcgccaaca tgcaggcgat cactgaacgt tttggcccgt cgcacatggc
    16321 gttcctggta gtgccgatgg tcggtgcgtt ctttattgat atcgttaacg cgctggtgat
    16381 taagctgtat ttaatgttgc cgatttttgc gggctaaccg atgaagcggc ggtcttctca
    16441 ccgccgcctt ttacgcattc gagtaacgtt ccgtctccgg catccagcgt tctatcaggg
    16501 cttttgcttg ttgtgggtaa cgttcgtgaa tatgtcgcgc caggcgctga acttccggga
    16561 tcatcgcctg atcgcgcagt aaatccgcca ctttaaactc agcattaccc gtctgacgcg
    16621 tacctaacag ttcacccggt ccgcgaatct ctaagtcttt ctgtgcaatc acaaaaccgt
    16681 cgttgctgtc gcgcagcact tgcagccgaa tttgcgcggt tttggagagc ggcgttttgt
    16741 agagcagcac gcagtgagaa gccaccgcac cacgacctac acgcccgcga agctggtgca
    16801 actgcgccag acccagacgc tccgggtttt cgataatcat caggctggcg ttaggcacat
    16861 caacgccgac ttcaataacg gtagtggcaa ccagcaggtg taactcgcct tgtttaaatg
    16921 atgccatgac ggcctgcttc tcggctggtt tcatccgtcc gtgtaccaga ccaacattca
    16981 actctggtag cgccagtttc aactcttccc aggtagcttc cgccgcctgc gcttccagta
    17041 actctgactc ttcaatgagt gtgcacaccc agtacgcctg acgcccttca gtcatacagg
    17101 cgtggcgcac gcggtcgata atgtcgttac gacgcgtatc cgggattgcc actgtcgtca
    17161 ctggcgtgcg gcctggcggc agctcatcta tcaccgaggt atcgagatcg gcatacgcgg
    17221 tcatcgccag cgtgcggggg atcggcgtcg cggtcatgat caactgatgc ggatggaagc
    17281 cctgctgctg gcctttctcc cataacgcca gacgctgatg cacgccaaaa cgatgctgtt
    17341 cgtcgataat caccagcgcc agaccgttaa actgcacctg ttcctggaag atggcgtgtg
    17401 taccgacaat catctgcacc tgaccgctgg cgatggcttc ctgctgtgcc agtcgcgctt
    17461 tacctttctg cttaccggcg agccagccca cttcgatacc gagcggtgca aaccagttgc
    17521 ggaagttatt cgcgtgctgc tcggcgagta attcggttgg tgccatcaat gccacctgtt
    17581 tgccgtgggc aatcgcacgc aacgcggcga gggcggcgac cagcgtttta ccggaaccta
    17641 catcgccctg caccaggcgc atcatcggcg catccagcgc catatcgcgc tcgatctccg
    17701 ccaccacgcg tgcctgtgcg cccgttggct tgaacggtaa ggctgcgagg agtttatttt
    17761 tcagcgcgtc attggcgcta agcggctggg catggaaacg ttgtgcacca gcgcgtaacg
    17821 ccaacatgct gaggttgtgc gcaagcagtt cttccagaat cagacggcgt tgtgctggat
    17881 gctgcccggt ttcgagatcg ctaagctgta gcgtcggcgg cgggcggtgc aaagtgcgca
    17941 acgcttccgg tagcgtcatc attccttgtg acagttccgg cggcaggagt tcttcaatgg
    18001 cgcaggtgtc gagcagatcc agcgcctggt cggttaattt acgcagcgtg gcctgcttta
    18061 cgccttcggt tgttgggtaa accggcgtaa gcgtttcctg caactctggc gtactgagat
    18121 cgccctgcac gcggtattcc gggtggatca tctccgcgcc atatttaccg cgctttgctt
    18181 ctccataggc cagcacgcgg cggcccgtcg ccaggctatt tttcattgcc gcgttgaagt
    18241 tgaaaaagcg catggtgaga atgccggaac cgtcgctgat ctggcaggtc atcatccgcc
    18301 gaccgccgaa ggagatattg cagttcagca cttcgccttc caccgtggca taaacgcccg
    18361 gtagtagttc tccgatgggg tagagatggg tgcgatcttc gtagcgcagg ggaaggtgta
    18421 agagtagatc ctgcacggta tgcaggttga ttttcgccag cttgttacta agcgctgcac
    18481 caacgcccgt tagggaactg agcgggacag catctaacag gcgacctttc atggtactta
    18541 ccctgcagct tgcatagtgg cccaccagtc ggcatcggct tcaatttcgc cctgctgatt
    18601 gacgtggggg taaggcaggc ctttgcgttt tgcgactttc gccagcaccg gatagccgcc
    18661 ttcaaacagc aggcgttgtt gctctgcttc cggcaacatg ctgttttcac gcaggtacat
    18721 gcccgcattt tgccgctggc gctgggcttc gtaaagaatg agggctgagg caacggaaac
    18781 attcagcgat tgcaccatgc caatcatcgg aatgatgatg tcccgatccg ccagggccaa
    18841 cgcttcctgc gtgatgcctg ttttctcctg tcccatcaaa atacaggtcg ggcgagtgta
    18901 atcaatttcg cggaaatcga cagcgttatc agaaagatgg gttgccagaa tctgcatgcc
    18961 ctggcctttc agatgagcga cggcatcgcc aatggtgcgg tgcgttttca cctgtaccca
    19021 gctgttacta cccgcagctg ccgaagccat ggtgcgcatc cggctaccag gccagacagc
    19081 atgaacttca tgtacaccaa cggcatctgc ggtacgaata atcgcagaaa cgttatgagg
    19141 tttgtggacc tgctccatgc agacggtcag atcaggctgc cgcctggcga gcatttcgca
    19201 gatacgtgca taacgtgttg ggttcataaa acattaattt cggtttcggg tgactttaat
    19261 cacgtctggc atcacgcgga ttttgcgcat gatattcgcc agatgcacac ggtcacgggc
    19321 ggtcagacga ataaaggcgc tgtagacgcg accatctttc tcttccgtat tcaaactttg
    19381 aatattcgag gtggtggtgt taattgccgc tgtcaggttt gccagcgcgc cctgatgatt
    19441 gaacatctcc accttgattt cggtgatgaa ctcctgcgcc gtctctttat cccattccac
    19501 agccataaac ttctctggct ctttctggta gccacggatg ttacggcagg attcatggtg
    19561 gatcaccaga cctttaccgg ggctgacgtg ggcgataatc gggtcgccag gaatagggcg
    19621 gcagcatttc gcaaaggtga tcagcacgcc atcggcacct ttaatgggca gatgtccgtg
    19681 actttgggtt gccggtggaa tggaggcgtc cccatgttgc agatttttcg cgaccaccac
    19741 gctcattgcg ttaccaaggc cgatttcagc cagcagatcg tcaagcgttg ccagcttcat
    19801 gcggtccagc tcgcgctgaa tattttcctg cgggatttca ttgagcttgc ggctgccacc
    19861 caacgcatgg ttgagcagac gacggcccag gcttacagaa tcatcacgct tgaggttttt
    19921 cagcaattga cgaattttgg cgcgcgcttt cgagctaacg acaaagttca gccacgcggc
    19981 attcgggcga gcgcccggtg cggtaataat ttcaacggtt tgaccgctgg tcagcggctg
    20041 cgacagcggg taaggctggc ggtcgacacg cgcgcccacg caggcatgac cgatatcggt
    20101 atgcaccgca taagcgaagt cgacgggcgt tgcaccggca ggcagctcta caatgcgccc
    20161 ttccggtgtg aaaacgtaaa tttcatccgg gaagagatcg gatttaacgc tctcgataaa
    20221 ttcaaacgag ctaccggcac tctgttgcag ctccagcagg ctttgcatcc agcgctgggc
    20281 gcggatttgt gcggtagtac tggtttcgcc gtgctcttta taagcccagt gcgcggcaac
    20341 acccatctct gccatctgat ccatatcttc ggtacggatc tggacctcaa ccggcacgcc
    20401 gtgcgggcca atcatcgagg tgtgcaaaga ctgatagccg ttcgcttttg gaatggcgat
    20461 gtagtctttc acgcgacccg gacgcggctt gtacaggcta tgcatttgcc cgagcacgcg
    20521 ataacaggtg tcggaatcat tgacgatcac gcggaaggcg tagatgtcca tgattgagtg
    20581 aaaacgctgc tctttgagca ccattttgca gtagatcgaa taaagatgct tttcgcgacc
    20641 actgacgcgg cacggtattc ccgcttcctg caaacgccct tcgatttcag aaagaatctt
    20701 ctggatcatc tctttacggt tgccgcgcgc ggctttcacc acttctttga ttacgcgata
    20761 acggttggga tacagcgcct caaaacccag ctcttcgagt tcggttttaa tgtggtggat
    20821 acctaaacgg tgcgccagcg ggctgtaaat ttcgagtgtt tcacgggcga tgcggcgacg
    20881 tttgtccggg cgaagtgagc ccagcgtgcg catgttgtgg gtacggtcgg caagtttgat
    20941 gagaatgacg cggatatcct gcaccatcgc cataatcatc ttgcgaaagt tttcggcctg
    21001 cgcctctttc ttatcgcgga acttgagctt atcaagtttc gacaccccct ctaccagctc
    21061 ggcgacgctt ttaccaaaaa gctgttccat atcctgatag gtggcgggtg tatcttcaat
    21121 cacgtcatgc agcagcgccg ccattagcgt ttcatagtcg agtttcatct cggccagaat
    21181 gcaggcaacc gctaccgggt gcgtgatata gggttcaccg cttgaacgtg tttgcccctc
    21241 gtgagcatca cgtgcaacga gatacgcctg ccgcagacgc ttgatttggt cttccggcag
    21301 gtaggtttga atcagttgat tcaggctttc aaacagatac aagggcgacc cgctttgtga
    21361 ttaacgacga ccttcagcaa tagcggtaac ggcttgtaat tcagcggctt cctgctcttg
    21421 ctgttcctgg cgttcgcgaa cgtcgaggat ctggttgttg atcagacctt cttcgatttc
    21481 gcgcagcgcg attacagtgg ttttatcgtt ttcttccggt accagcggat cctttccgcc
    21541 tacctgcatc tgacgagcgc gacgcgcggc gaccagtacc aggtcaaaac ggttaccaat
    21601 tttctctaca gcgtcctgaa cagttacgcg tgccatactt aaaaagctcc acaggtgaag
    21661 aaatgactgg gcatgatact gaaatcaggt tcagtctgcc aacaatttgc tgattaaagc
    21721 gtcatgacgc tgcttttggc ggctcatgcg cagacgttcg gcgcgaataa tggtcttcaa
    21781 atcggtcaac gcggtatcga agtcatcatt cacaatcagg taatcatatt cggcgtaatg
    21841 gctcatttct gcaacagctt gcgccatacg ctttgcaatg acctcttcgc tgtcctgacc
    21901 gcgaccgcgt agacggcggt ccagttcaat tttggacggc ggtaaaataa agatactccg
    21961 cgcgtgcggc atcttctggc gaatttgctg cgcaccctgc cagtcgatat cgagaaaaac
    22021 atcgacaccg gtcgccagga cttgctcaat ggcctcacgc gaagtgccat agtaattacc
    22081 aaaaacttct gcgtgttcga ggaacgcatc tctgctaatc atttctttaa attcatcatg
    22141 attaacaaag aaataatgtt caccgtggac ttcaccagga cgcggttggc gtgtggtgtg
    22201 tgaaacagaa acctgggtgt catacaacgg ttgggttttt aataaagcct gaatcaggct
    22261 ggatttaccc gcgccactgg gggcagaaac aatataaagc gtgccttgag ccatgagtat
    22321 ctttcgtatg tgattagcga aataaagcct acatacgagc ttattataca cggcgcgggc
    22381 atgtgacgta gcccttgctc acactttttg cgcaaagttg ttgcgttttg cgtagctgct
    22441 tccgcatttg cctgtcattc actgcaataa agacgattct caggaagcgt cctcgcagac
    22501 tccgtttttg ccactcggct ttgctttcat cacgttgtat atctgcctga ctatctacag
    22561 cggaggtgat gatgaaagta tggatggcga tattaataag tatcttgtgc tggcaatcat
    22621 cggcgtgggc agtctgtccg gcctggtcgc cagccagagc acaggaagaa atttcccgcc
    22681 tgcaacagca aataaaacag tgggacgatg actactggaa agaagggaaa agtgaggtgg
    22741 aagacggtat ttacgatcag ttaagcgccc gtcttacgca atggcaacgc tgttttggga
    22801 acgagacccg cgatgtgatg atgccgcctt taaacggtgc ggttatacat ccggttgccc
    22861 atacgggtgt acgcaaaatg gcggataaaa tcgcattaag tctgtggatg cgcgaacgta
    22921 gcgatctttg ggtgcagcca aaagttgatg gcgttgcggt aaccctggtt tatcgggacg
    22981 ggaaactgaa caaagcaatc agtcgcggca atggcctgaa aggcgaggac tggacgcaga
    23041 aagttcgctt aatttccgct gtgccgcaaa ccgctagcgg gcctttagcc aacagtacgc
    23101 ttcaggggga aatatttctc aaacgcgagg ggcatatcca acaacaaatg gggggaataa
    23161 atgcccgcgc aaaagttgct ggtctgatga tgcgccagga cgatagcgac acgctgaatt
    23221 ctctgggcgt ttttgtctgg gcatggccgg atggaccgca gttaatgacc gatcgtttaa
    23281 aagagctgac taccgctggt tttactctaa cgcagacgta tacccgtgcg gtgaaaaatg
    23341 ctgatgaagt tgcgcgcgta cgcaatgcgt ggtggaaagc gaaattaccc ttcgtcaccg
    23401 atggcgtggt tgtgcgagcg gcgaaagaac ccgaatcccg ctattggcta ccaggccagg
    23461 cagaatggtt ggtggcctgg aaatatcaac cggtagctca ggttgccgaa gtgaaggcga
    23521 ttcagtttgc ggtgggtaag agcggtaaaa tatcggtggt tgcgtcactc gcacctgtca
    23581 tgcttgatga taaaaaagtc cagcgggtga atattggttc cgtcaggcgc tggcaggagt
    23641 gggatattgc gcctggtgat cagatcctcg tcagccttgc cggtcagggt attcctcgca
    23701 ttgatgatgt ggtgtggcgc ggtgcagaac gcacaaaacc gacaccgcca gaaaaccgct
    23761 ttaactcgtt gacctgctac tttgcttctg atgtttgtca ggaacagttc atttcacgct
    23821 tagtctggct ggggtcaaaa caggttcttg ggctggatgg cattggtgag gccggctggc
    23881 gcgcgctgca tcagactcat cgctttgagc atatcttttc ctggctttta ttaacgccag
    23941 agcaattaca gaacacgccg gggatcgcga aaagtaaaag tgcgcagtta tggcatcagt
    24001 ttaatctggc tcgtaagcag ccttttactc gctgggtgat ggcaatggga ataccgctaa
    24061 cccgggtggc gcttaatgcc agtgatgaac ggtcctggtc gcaactttta ttgagcacgg
    24121 agcagttctg gcagcagctg ccggggactg gatcggggcg cgccagacag gttattgaat
    24181 ggaaggaaaa tgcgcaaatc aagaagctgg gcagttggct ggctgcccag cagatcacag
    24241 gttttgaacc ttagtgtcct tcgtggctgt agtaaaagac cggtaatccc agcttaaggc
    24301 gtagcgccag taaacgggca aaaaagccga ataccagggt ggagataatt accacatcat
    24361 gattagaaac atagtgttgc agcgcgatgt acagcacggc ggaggcaaat gagacaccgg
    24421 catacaattc tttctggaat accagcggga tgcgtttaca gaacatatcg cgcaaaacgc
    24481 cgccaaatac gccggtcgtc acagctgcga caacggcaat gatcgggccg tgtcccatat
    24541 ccagtgcaac ctgtgcgcca atgatagaaa agaccaccag tccgagcgca tcgagcacca
    24601 gaaacacttt gcgcaggtaa ggcattacag gagcaacgat agtggtgagt actgcggcgg
    24661 tagcgacaat aatcacatat tccgggtgtt tgacccagcc gagcggatag tggcccagca
    24721 gaatatcgcg cactgaccct ccgccaattg ccgtggcggt cgcaataata attacgccaa
    24781 atgtatccat gcgccgtcgt ccggccgcca gcgcccccgt catggcttcg gcagtaatgc
    24841 caaccaaata caaaatgtgt aacagcatgg ttatctccct aaaaatctgt ctggcagggt
    24901 agcgatttgt acgcattgtc acgattgaga ttttttaagg tgagaggaaa ttgataattt
    24961 ttagttatgt gaaatcgttg ttttctgtag cgtagatgca ttcttctgac ttcttgtctg
    25021 agaaaataca taagaaaaag taggttaaaa ctgaggggtg gaaaagaaag gtgcaatgtt
    25081 agccattgtg ttcaccgcac atggactttg gcttacgcat cgggtatctc aatcaacaaa
    25141 aagagttttt agtttcggct attttttctc tgtaattttt acactgtttt ccaactgctt
    25201 gatgctgatc tgcggggtcg gcaactcctc aggcatggtt ccgccaagtt cctgaatggt
    25261 ttgcctcacc ttgcgaccca cgtcaaagtg tgtggtgtta gcttgctgtt ttgaattcac
    25321 gccatcccgc ttgagttttt cttctgtttg ggtagctcga aagagattag ccgccagttc
    25381 tgttgaaccc atatgatcca ggatcttctg actctttttc agccccttcc gctgatggat
    25441 ggctttctga tctaatccac catacagccc ccggtaacca tgattctgaa atatggcgaa
    25501 atcgatagct gtcgctaccc cagcttgctg cgctgcctca actaattgtt tgttatgttc
    25561 tttcaactca tttctcagaa aaagacgctt ttcgtcttca cgaagttgcc tgaaagcttc
    25621 atcgtcagcc agctcctgtc gtcgggtctg gatagcaaaa taagtttgcc ctgccgcaat
    25681 aaccggtttc gcagggtcgc cgttttgtac taccaggtaa caggcatagc gggagagtac
    25741 gatgtctttt aatgctcttt tggcatttga gccaattttg accatatcga ggatctcctc
    25801 gaaatggtca tcaattgtgt gaccactatt cagacaagct tctttggcgc gcgttaatac
    25861 aggtataaag tgacgatact ctgaataacc cagtagtttc ccaagttgtc ttgctgacca
    25921 ttgttctgct ccgtttgcat taatcagctt tatctcttca aaaggttgat gatgttcgtt
    25981 cattgcccac tccatgtgat atcgattgca aggaaagtaa gtctacaact gtgtttatat
    26041 acagttgtga gtatctcacg ttctcactgc gctgggcaaa aaaacatcgc aaaatctgga
    26101 gcaggcacgc aaaacagaat tgaatgttgc ggggaagaaa aatgccgctc tgttttagtg
    26161 gcggcatttg tgaaggcgga aaagtaagaa ttggcgttac gagttactcg atattctgga
    26221 tctgctcgcg catctgctca atcaacactt tcagctcaat cgcggagttt gtcacttcgg
    26281 cattgataga cttcgacgca agagtgttcg actcgcggtt gaattcctgc atcataaagt
    26341 ccagacgacg accaacggct tcttttttct tcagaatgtt gtaggtctct ttgacatgcg
    26401 cttctaggcg gtccagttct tcggcaacgt caattcgttg tgccagcaga accaactcct
    26461 gctccagacg gttgttttcc aactgaacct gagcatcttc cagcttcgcg accagacgct
    26521 cacgctgcca ttgcaggatt tccggcatat gggcgcggac tttgaccact tcggcggtga
    26581 cgccttccag acgctgctcg atcaatgctt tcagcgcctg accttcggtt tcgcgcgcga
    26641 cgataaagtc gtccagcgta ccatccaacg ccgcgagaat ttcagcggca atcgcgtcga
    26701 gatcctgctc ctgggctgcc atcacgcccg gccagcgtag aatatcaacc gggttgattt
    26761 ccccttcgtc actctgcatt tttacccagt tcgcggcagt taccagctgt ttagccagtt
    26821 tttcgttgag gatcagctca ccttgtgcgc taacatctgg ttcatagcgc agggtacatt
    26881 ccactttacc gcgcgtcagg cgagaacgaa tacgctcgcg aacgacaggt tcaaggctac
    26941 ggaactgctc cggcagacga aagtaagttt ccagataacg ctggtttacc gagcgcattt
    27001 cccaggttgc gctcccccat tcacccttga tttcacgccg ggcgtaggcg gtcatactgc
    27061 ggatcataga cgttcctgtt tataaaagga gaggtggaag gattatagcc atcgatgcct
    27121 tgtcaggata ggaataaccg ccggaagtcc gtataatgcg cagccacatt tgtttcaagc
    27181 cggagatttc aatatgcgtc cagcaggccg tagcaataat caggtgcgtc ccgttaccct
    27241 gactcgtaac tatacaaaac atgcagaagg ctcggtgctg gtcgaatttg gcgataccaa
    27301 agtattgtgt accgcctcta ttgaagaagg cgtgccgcgc ttcctgaaag gtcagggcca
    27361 gggctggatc accgcagagt acggcatgct gccacgttct acccacaccc gtaacgcgcg
    27421 tgaagcggcg aaaggtaagc agggtggacg caccatggaa atccagcgtc tgatcgcccg
    27481 tgctcttcgt gcggcagtag atttgaaagc gctgggtgag ttcactatta cgctggactg
    27541 cgacgtgctt caggctgatg gtggcacgcg taccgcttcg attacgggtg cctgcgtggc
    27601 gctggcagat gcgctacaga agctggtgga aaacggcaag ctgaaaacca acccgatgaa
    27661 agggatggta gccgcagttt ctgtcgggat tgtgaacggc gaagcggttt gcgatctgga
    27721 atacgttgaa gactctgccg ctgaaacgga catgaacgtg gtgatgactg aagacgggcg
    27781 catcattgaa gtgcagggga cggcagaagg cgagccgttc acccatgaag agcttctcac
    27841 cttgttggcg ctggcccgag ggggaatcga atccattgta gcgacgcaga aggcggcgct
    27901 ggcaaactga tttttaaggc gactgatgag tcgccttttt tttgtctgta gaaaagtaag
    27961 atgaggagcg aaggcatgaa accatatcag cgccagttta ttgaatttgc gcttagcaag
    28021 caggtgttaa agtttggcga gtttacgctg aaatccgggc gcaaaagccc ctatttcttc
    28081 aacgccgggc tgtttaatac cgggcgcgat ctggcactgt taggccgttt ttacgctgaa
    28141 gcattggtgg attccggcat tgagttcgat ctgctgtttg gccctgctta caaagggatc
    28201 ccgattgcca ccacaactgc cgtggcgctg gcggagcatc atgacctgga cctgccgtac
    28261 tgctttaacc gcaaagaagc aaaagaccac ggtgaaggcg gcaatctggt cggtagcgcg
    28321 ttacaaggac gcgtaatgct ggtggatgat gtgatcaccg ccggaacggc gatccgcgag
    28381 tcgatggaaa ttattcaggc caatggcgcg acgcttgctg gcgtattgat ttcgctcgat
    28441 cgtcaggaac gggggcgcgg cgagatttcg gcaattcagg aagttgagcg tgattacaac
    28501 tgcaaagtga tctctatcat caccctgaaa gacctgattg cttacctgga agagaagccg
    28561 gaaatggcgg aacatctggc ggcggttaag gcctatcgcg aagagttcgg cgtttaaaga
    28621 aactcgccgg atgaaaagtc atccggcgtc atattactgc aactgtgcgg caattagcgg
    28681 ccagcgggcg tcaaaatcat ccgtcgggcg gtatttaaac tcgctgcgga caaagcgtga
    28741 cagcatacct tcacagaagg ccaggatctg gcttgccagc agggtttcat cggtggtgta
    28801 accttcaccc tcacgcattc tcttttcgcg caatacctga cgcaactgcg cttcaatacg
    28861 ctcgaacagc tggttgatgc gcccttgcag gcgatcctgt tcaaacatta gtgcatgacc
    28921 agtgaggatg cgggtcaggc caggattacg ctcaccaaaa ccgagaagca gcaacacaat
    28981 cagacgcagg cgcgctgtgg tgtccttctc atctttcaga atcagattga tgcgagtaat
    29041 caggctatct tcgataaact caatcaggct atcgaacatg cgggtcttac tggggaagtg
    29101 gcgatacagt gccgcttcgg aaacgccgac agaggcggcc agttttgccg tcgtgatacg
    29161 ttggcttcca tcgctggatt ccagcatcag cgccagagac tgaagtattt cctcgcgacg
    29221 gttccttttc gcagtttgtt tttctgccat gttacaaaat acccctgaaa ataagcactt
    29281 gccaggcggc acccacgcta tgaccgcaaa cgaaatgttt gcggctatgt tatgacgtta
    29341 ttcggatgcg tatgtgttac tgacgaccag agtgaccaaa gccgccttca ccgcggtcgg
    29401 tggcgtcgaa atcttccacc agattaaatt cagcctgtac taccggaaca aaaatcatct
    29461 gggcgatgcg ttcgccaggt tgaatggtga agctgtcctg accacggttc cacacggaaa
    29521 tcatcaactg gccctgatag tcagaatcga tcaatcctac caggttacca agcacgatac
    29581 cgtgcttatg tcccaatccg gagcgcggca gcatcattgc cgccagtgaa ggatcggcaa
    29641 tatgaatcgc cagcccggtc ggaaccagcg tagtgtcacc cggagccagt tctacggcgt
    29701 cgtcgagaca ggcacgcagg tcaagtccgg cagagccaga ggtggcataa gtcgggagcg
    29761 gaaattcctt cccaacgcgc gggtccagaa tcttaacgtc gatttttttc atcataacgg
    29821 gtcacgatct cgtcgagtaa taattggcca aggagctctt tgcgctcaag cggtaagact
    29881 ttatctccgt cctgccagaa aaggtgtaat gcgttgttgt cgctgttaaa tccttgagtt
    29941 ggctgggaaa catcgttcgc gcagatcaga tcaaggtttt tacggatacg tttttgccgg
    30001 gcgtattctt ccacattatt tgtttcggcg gcaaatccaa cgacgtaggg tcgatggtct
    30061 tttagtgcgg caacgcctgc gacgatatcg gggtttttaa ccatttttat tgttaattca
    30121 tcaccctgcg tggcctgctt tttgattttc tctggggcca cggtagctgc gcgataatcc
    30181 gccacggcgg cgcaaccgat aaaaatattt tgctgctgta cagaagcatt cacggccgct
    30241 tccatttcca gcgcagtcat cacatcaaca cgtttaacaa acggtggcgt cggtagtgaa
    30301 accggacctg ataccagcgt gacgttcgcg ccacgacggg cagcggcggc ggcgatagca
    30361 aaacccatct tgccggagct gtgattagag atataacgca cgggatcgag cggttcacgc
    30421 gtcgggccgg cggtaatcat aatgttcaga tgtttcaggt cgttgacggg cgaaaaatgc
    30481 gccaccgcca tatccacaat ggttaacgga tcgagcattc gcccaggacc gatatcacca
    30541 caagcctgac tgccactgtc tggcccccag atgagcaaac cacgggaagc aagcacctct
    30601 aaattatgct gcgtggcagc ggcacggtac atctgctggt tcatggcggg gagcacggct
    30661 acaggcgcag gtgtagccag acaaatcgtc gataccaggt cattcgccat tccggcagca
    30721 acacgggcaa tcaaatctgc cgtggcaggg gcgagaatca ctaaatcagc ccatttaccc
    30781 agctcaatat ggcccatagc ggcttcggct gctgggtcca gcagactgtc ggaaacggga
    30841 taaccagaaa ccgcctgcaa gctaagtggg gtgataaagg cttttgccgc ttcggtcatg
    30901 gctacgcgga cgtcggcccc gcgctcgcgc aaacgacgca ccagttcagg ggttttatag
    30961 gcagcaatac cgccgctgac gccgagaacg atttttttac cggccaggct catgatgatt
    31021 tttcctgttg ggtgacacca gaagttggcg attttatcac aatacttttg ttgtcgtgcc
    31081 ttcacctgag aatcactttg cgaggcgctt tccaggattg aaaactggcc gtcgatttaa
    31141 cggaacggct atgacaggat gcgagcacca caaaggaggt gaaggtgaaa aacaatgcac
    31201 agctgttgat gccgcgcgaa aaaatgctga agtttggtat tagcgcctta acggatgtcg
    31261 agctgctggc gctatttctg cgtaccggaa cgcgcggtaa agatgtatta accctggcaa
    31321 aagagatgct ggagaatttc ggctctcttt atggcttgtt aacctctgaa tatgaacaat
    31381 ttagtggtgt tcatggaatt ggcgtggcga aatttgccca gttaaagggg attgctgaac
    31441 tagcgcggcg ttactacaac gtgcggatgc gtgaagaaag ccctttactc agcccggaga
    31501 tgacgcggga atttttacaa agccagctca cgggggagga gcgggagatc tttatggtga
    31561 tctttctcga ctcacaacac cgggttataa cgcatagccg tcttttttcc ggcacgctaa
    31621 accatgttga agtccatcct cgggaaatta tccgcgaagc gataaaaata aatgcctcgg
    31681 cgctgatcct tgcacataat cacccttcgg gttgtgctga acccagtaaa gcggataaac
    31741 tcattactga acggataata aagagttgtc agttcatgga tttacgcgtg ctcgaccata
    31801 tcgtgattgg gcgtggagag tatgtttctt ttgccgaacg cggctggatt taacccgcta
    31861 tgcgcgatcc ttcgggatct ttgtctgttc gggacttgag cacatcgctg agtcagcgta
    31921 tactacgcca cctttgagaa tctcgggttt ggcatttggg cctggcaatc gagagttcac
    31981 atagaactgc gatgaccggg ctgtaaagcc tgacgaggcg ccaatacccc atacgaagct
    32041 cgagctaatt tgatttttgg agaatagaca tgtcccgagt ctgccaagtt actggcaagc
    32101 gtccggtgac cggtaacaac cgttcccacg cactgaacgc gactaaacgc cgtttcctgc
    32161 cgaacctgca ctctcaccgt ttctgggttg agagcgagaa gcgttttgtc accctgcgcg
    32221 tatctgctaa aggtatgcgt gtaatcgata aaaaaggcat cgatacagtt ctggctgaac
    32281 tgcgtgcccg tggcgaaaag tactaagtac ttagaggaaa taaatcatgg ctaaaggtat
    32341 tcgtgagaaa atcaagctgg tttcttctgc tggtactggt cacttctata ccactacgaa
    32401 gaacaaacgt actaagccgg aaaaactgga actgaaaaaa ttcgatccag ttgttcgcca
    32461 gcacgtgatc tacaaagaag cgaaaatcaa ataatttccg ctttgatgta acaaaaaacc
    32521 tcgctccggc ggggtttttt gttatctgct tgcccccata ttgactgcat ctgttcattc
    32581 ctggagatgc tatgcctgaa ttacccgaag ttgaaaccag ccgtcgcggc atagaaccgc
    32641 atctcgttgg tgcaaccatt cttcatgcgg tggtgcgcaa cggacgcttg cgctggccgg
    32701 tttcagaaga gatctaccgt ttaagcgacc aaccagtgct tagcgtgcag cggcgggcta
    32761 aatatctgct gctggagctg cctgagggct ggattatcat tcatttgggg atgtctggca
    32821 gcctgcgcat ccttccagaa gaacttcccc ctgaaaagca tgaccatgtt gatttggtga
    32881 tgagcaacgg taaagtgctg cgctacaccg atccgcgccg ctttggtgcc tggctgtgga
    32941 ccaaagagct ggaagggcac aacgtgctgg cccatcttgg accggagccg cttagcgacg
    33001 atttcaacgg tgagtacctg catcagaagt gcgagaagaa aaaaacggcg attaaaccgt
    33061 ggctgatgga taacaagctg gtggtagggg tagggaatat ctatgccagc gaatctctgt
    33121 ttgcggcggg gatccatccg gatcggctgg cgtcatcact gtcgctggca gagtgtgaat
    33181 tgctggctcg ggtgattaaa gcggtgttgc tgcgttcgat tgagcagggc ggtacaacgc
    33241 tgaaagattt tctgcaaagc gacggtaaac cgggctattt cgctcaagaa ttgcaggttt
    33301 acgggcgaaa aggcgagccg tgtcgtgtgt gcggtacgcc gattgtggcg actaaacatg
    33361 cgcagcgggc aacgttttat tgtcggcagt gccagaagta attgatgcgc gccggatggc
    33421 ataccatccg gcataaacgc tacgctaact tcgccatcag cgcctgatgg acattctccg
    33481 gcaggaaatg ggtgacatcg ccctgatggc gcgccacctc tttcaccaac gatgaagaga
    33541 taaacgacca ctctttcgac ggcatcagaa acacactttc cagttccggc attaagtggc
    33601 gattcatatg cgccagctgc atttcatatt caaaatccgc caccgcacgc aggccacgaa
    33661 tcagcaccgt agcgtgttga ttacgggcga agttcgccat caaatcacta aacccgacca
    33721 cttccacgtt ccccagatgc gcggttgcct gctgtgccag cgccacacgc tcttccagag
    33781 taaacatcgg ttttttactg gggctggcgg caatcgccag aataacgtga tcgaacattt
    33841 gcgtggcgcg cgtcacgata tcgatatgac cattggtaat gggatcgaaa gtacccggat
    33901 aaatcgcccg tttttgcata acaacctcaa tgcgttttcg gtggcaggta aggttccagc
    33961 agttgaagca ggcgctgtag cgcgccctgg ttttgataca gtacttcaac ggcatgacgg
    34021 ccatagaaac tacggtaatc ggcatcggtg agtaaagagg aaacctcttt tgcaagcgta
    34081 gtggcatcgg taacggtaat cagcccgctt gcctgttcca gccgcgcgca aatgtcttta
    34141 aagttaaaag tatgcggccc catcaatacc ggaatagcgt gtgcggcagc ttccagcgga
    34201 ttatgcccac cacgttcaac cagtgaaccg ccaacaaagg cgagatcggc aatgccgtac
    34261 agtaacatca gctcgcccat cgtatcgcca accacaacct gcgtgctggt ggaagggact
    34321 tcccctgaag agcgtgtgat atagcttagt ccagcctggc ggacaaggtt aatcgcatcc
    34381 gggaagcgtt ccggatgacg gggtaccagg atgagcaata aattagggaa ttgctgtaac
    34441 aatgcctgat gtgcggcgat caccacactc tcttcgcctt cgtgagtgct ggtggcaatc
    34501 cataccgggc ggtgtggtgc ccactggcgg cgcagcgtca cggctttagc agccagttgc
    34561 ggcgttacag aaatatcaaa tttcaggcta ccggtaacgg tcacctgatt attttttgcg
    34621 cccagcgcca caaaacgcgc gccatcttct tcattttgcg cagcaatcag tgtaatacga
    34681 cgcagcaagc ggcggacgaa tttacccagt ttggcgtaac ctgcggccga gcgggcagag
    34741 agtcgcgcgt tagcgatcac cagcggaatt ttacgtttat gtagcgccgc aatcaggtta
    34801 ggccatagtt cggtttccat aatcaacacc agtttagggt cgactttatt caggaaacga
    34861 ttaagtgcat cgggcaggtc atacggcaga taaacgtgct gaacatcctt cccgaaagcc
    34921 gattgtacgc gctccgaacc ggttggcgtc atggttgtaa cggtaatcgg taaatcagga
    34981 taacgatgac gcagcgcgcg caccaacggg attgccgcca gagtttcacc gacggagacg
    35041 gagtgcagca taatgccgcc tggttttagc ggatggcggt aaaaaccgta acgttcaccc
    35101 cagcgttttc gataggccgg agccttacgt ccgcgcaccc agagccgtat ccagatcagc
    35161 ggctgaataa ggtagagaag ggcggtgtaa agcaattcga gcatagtaaa tagctgactt
    35221 atggatgtgc tggggattct atgtatttag ctgtggcttt accattactt ttcccgtttt
    35281 tgacttaaat agcaatttac ccgccaattc ttgctgaaca tctatacttt ttttgtattt
    35341 ttatgcgatt cattgatttc tgcagtacgt gcaggaagga atcgcagagc aacagtgaaa
    35401 gaatatggtt gctaatgatt ttatttgatt gatttcttta ttgaaaatca acaatgtgct
    35461 taaaaatcat tcgggtcgga ttgccgcgaa agaagtgata cactagcgcg tcaatatcag
    35521 tgcggtatca gtattcaggt agctgttgag cctggggcgg tagcgtgctt tttctgcttc
    35581 accttaatcg gataatctca acaaaaagag ttacttgtgg ataagccatt tcgaagaatt
    35641 ttgctcatta agatgcgttt tcatggggat atgttattaa ctactcccgt cattagttcg
    35701 ctgaaaaaaa attaccctga cgcaaaaatc gatgtgctgc tttatcagga caccatcccg
    35761 atcctgtctg aaaatccaga gattaacgcg ctctacggca taaaaaataa aaaagcaaaa
    35821 gcctcagaaa aaatcgccaa cttttttcat ctcatcaagg tattacgtgc caataagtat
    35881 gaccttatcg tcaatcttac cgatcaatgg atggttgcta tactggttcg cttattaaat
    35941 gcccgtgtga aaatttccca ggattatcat catcggcagt ctgctttttg gcgtaaaagt
    36001 ttcacccatt tggtgccgtt gcagggtgga aatgtggtgg aaagtaactt atccgtgctg
    36061 acaccattgg gacttgattc gttggtgaag cagacaacca tgagttaccc gcctgcaagc
    36121 tggaaacgta tgcgtcgcga acttgatcac gctggtgttg gacaaaatta tgtggttatc
    36181 caacctacgg cgcggcaaat cttcaaatgc tgggacaacg ccaagttttc cgctgtgatt
    36241 gatgccttac atgctcgtgg ttatgaagtc gttctgacgt ccggcccaga taaagacgat
    36301 ctggtctgcg tcaatgaaat tgcgcaggga tgccagacgc caccagtaac ggcgctggcc
    36361 ggaaaggtga ccttcccgga acttggtgcg ttaatcgatc atgcgcagct gtttattggc
    36421 gttgattccg caccggcgca tattgccgca gcagttaata cgccgctgat atcgctgttt
    36481 ggtgcgacag accatatttt ctggcgtccc tggtcaaata acatgattca attctgggcg
    36541 ggagattacc gggaaatgcc aacgcgcgat cagcgtgacc gaaatgagat gtatctttcg
    36601 gttattccgg cggcagatgt cattgctgct gtcgataaat tactgccctc ctccacgaca
    36661 ggtacgtcgt tatgatcgtt gctttttgtt tatataaata ttttcccttt ggcggtttgc
    36721 agcgcgattt tatgcgtatt gctcagacag tcgccgcccg aggtcatcat gttcgggttt
    36781 atacccagtc gtgggaaggc gaatgtcctg atgtatttga actgatcaaa gtgccggtta
    36841 aatcgcatac caatcacggg cgcaatgcgg agtattttgc ctgggtgcaa aaacatttac
    36901 gcgaacatcc cgtcgataaa gtcgttggat tcaacaaaat gccggggctg gacgtttatt
    36961 atgccgctga tgtttgttat gccgagaaag tagcgcagga aaaaggcttt ttctatcgcc
    37021 tgacgtcacg ttatcgccat tatgccgcct ttgagcgggc aaccttcgaa cagggcaagc
    37081 cgacacagct gctgatgctg acagataagc aaatcgccga tttccagaaa cattatcaga
    37141 ctgaagtgga gcgttttcat attctgccac cggggattta tcctgatcgt aaatatagcc
    37201 agcagccagc caatagccgt gaaatcttcc gtaagaagaa tggaataacc gaacaacaat
    37261 atttattgtt gcaggttggt tcagacttca cgcgtaaagg tgtcgatcgt tccattgaag
    37321 cacttgcttc gttaccggat tcgctgcgcc acaacacatt gctatatgtt gttgggcagg
    37381 ataaaccgcg aaaatttgag gcgctggcag aaaaacgcgg cgtgcgcagt aatgttcact
    37441 tcttctcggg gcgcaacgat gtctcggaat taatggcggc ggcggattta ttactgcatc
    37501 ctgcctacca ggaagccgcg ggaattgtac tgctggaagc gataactgca ggattaccgg
    37561 tactaacaac agccgtttgt ggctatgcgc attatattgt cgacgccaat tgcggcgagg
    37621 ctattgctga gccattccgc caggaaacat tgaatgagat tttacgcaaa gcgttaacgc
    37681 aatcttcatt gcgccaggcc tgggcggaaa atgcgcgaca ttatgctgat acacaagatt
    37741 tatacagtct gccagagaaa gcggcggaca tcataacggg tggtctggat ggttgaactt
    37801 aaagagccgt ttgccacgtt atggcgcggt aaagatcctt ttgaggaagt taaaaccttg
    37861 cagggtgagg tatttcgtga actggaaact cgccgcactc tgcgctttga aatggcgggc
    37921 aaaagctatt ttctcaaatg gcatcgcggc acgaccctga aagagataat caaaaattta
    37981 ctctcattgc ggatgccagt attaggcgca gaccgcgaat ggaatgcgat tcatcgactg
    38041 cgggatgtcg gcgttgatac tatgtatggg gtggcattcg gcgaaaaagg cattaatccg
    38101 ctcaccagaa cctcgtttat tataaccgaa gatctgacac caaccatcag tctggaagat
    38161 tactgtgctg actgggcgac taacccacca gatgttcgcg taaagcgtat gcttattaag
    38221 cgtgtcgcga cgatggtgcg cgatatgcat gctgcgggca ttaaccaccg tgactgttat
    38281 atctgtcatt tcctgctgca cttgcctttt tccggtaagg aagaggagtt aaaaatttca
    38341 gtaattgacc tgcaccgggc gcagcttcgc acgcgcgttc cacgtcgttg gcgcgataaa
    38401 gatcttattg gactttattt ttcttcgatg aatattggcc tgactcagcg ggatatctgg
    38461 cggtttatga aagtgtattt tgtcgccccg cttaaagaca ttatcaagca ggaacaaggg
    38521 ctgctgtcgc aagcagaagc aaaagccaca aaaatcaggg aaagaacgat tagaaaatcg
    38581 ttgtaatttc cggagagaaa tatgagtgcc cactatttta atccacaaga gatgatcaat
    38641 aagacaatca tcttcgatga aaggccagcg gcgtcagtgg catcatcatt ccatgtttct
    38701 tatggcattg ataaaaactt tctttttggt tgtggtgttt caatcacgtc agttttgtta
    38761 cataacaacg acgtgagttt tgttttccac gtttttattg atgctatccc tgaagccgat
    38821 atccagcgtt tagcccaatt ggcgaaaagc tatcgtacct gtatccagat ccatctagta
    38881 aattgtgaac ggcttaaggc attaccgacg accaaaaatt ggtctattgc catgtatttc
    38941 cgttttgtaa ttgcagatta ctttattgat caacaagata agatccttta cctggatgct
    39001 gatatcgcct gtcagggaaa cttaaagccg ctgataacaa tggatcttgc caataacgtt
    39061 gctgctgttg ttactgaacg cgatgctaac tggtggtcgt tacggggtca aagtctgcag
    39121 tgtaatgaac ttgaaaaggg ttactttaat tcaggtgtcc tgttaattaa tacactagcg
    39181 tgggcgcagg agtccgtttc tgctaaagcg atgtcgatgc ttgctgataa agccatcgtt
    39241 tcccgtttaa cctatatgga tcaagatatc cttaatctta tcctgttagg gaaagttaaa
    39301 ttcattgatg ctaaatacaa tacgcaattt agtttaaatt atgaattaaa aaaatcattt
    39361 gtttgtccaa ttaatgatga aaccgtatta attcattatg tcggcccgac aaaaccctgg
    39421 cattactggg ccggttatcc aagtgcgcaa ccttttatca aagccaaaga agcatcgccc
    39481 tggaaaaatg aaccgttaat gc
//
LOCUS       DACBVI010000025        37939 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.025, whole genome shotgun sequence.
ACCESSION   DACBVI010000025 DACBVI010000000
VERSION     DACBVI010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.025"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..793)
                     /locus_tag="IU325_06560"
     CDS             complement(<1..793)
                     /locus_tag="IU325_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP5808702.1"
                     /translation="MSKISGWNFSQNITSADNCKQKNEDLDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIKSSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLASAFNNGIREGHTVIIFTQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTITDLLE
                     KPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEIDDLNAVN"
     gene            complement(916..1368)
                     /locus_tag="IU325_06565"
     CDS             complement(916..1368)
                     /locus_tag="IU325_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HAP5808703.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPARVRKAVVKMLMA"
     gene            complement(1513..2106)
                     /locus_tag="IU325_06570"
     CDS             complement(1513..2106)
                     /locus_tag="IU325_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP5808704.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAISAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPLARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            2177..2890
                     /gene="bdcA"
                     /locus_tag="IU325_06575"
     CDS             2177..2890
                     /gene="bdcA"
                     /locus_tag="IU325_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP5808705.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     KRLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGEALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            3021..3416
                     /locus_tag="IU325_06580"
     CDS             3021..3416
                     /locus_tag="IU325_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAP5808706.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            3697..3831
                     /gene="pyrL"
                     /locus_tag="IU325_06585"
     CDS             3697..3831
                     /gene="pyrL"
                     /locus_tag="IU325_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HAP5808707.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            3835..4770
                     /gene="pyrB"
                     /locus_tag="IU325_06590"
     CDS             3835..4770
                     /gene="pyrB"
                     /locus_tag="IU325_06590"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HAP5808708.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            4783..5244
                     /gene="pyrI"
                     /locus_tag="IU325_06595"
     CDS             4783..5244
                     /gene="pyrI"
                     /locus_tag="IU325_06595"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HAP5808709.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            5317..5703
                     /locus_tag="IU325_06600"
     CDS             5317..5703
                     /locus_tag="IU325_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HAP5808710.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(5909..8605)
                     /gene="mgtA"
                     /locus_tag="IU325_06605"
     CDS             complement(5909..8605)
                     /gene="mgtA"
                     /locus_tag="IU325_06605"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP5808711.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(8746..8799)
                     /gene="mgtL"
                     /locus_tag="IU325_06610"
     CDS             complement(8746..8799)
                     /gene="mgtL"
                     /locus_tag="IU325_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HAP5808712.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            8984..9931
                     /gene="treR"
                     /locus_tag="IU325_06615"
     CDS             8984..9931
                     /gene="treR"
                     /locus_tag="IU325_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HAP5808713.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            10050..11471
                     /gene="treB"
                     /locus_tag="IU325_06620"
     CDS             10050..11471
                     /gene="treB"
                     /locus_tag="IU325_06620"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HAP5808714.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            11521..13176
                     /gene="treC"
                     /locus_tag="IU325_06625"
     CDS             11521..13176
                     /gene="treC"
                     /locus_tag="IU325_06625"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP5808715.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLGYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIISRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DESSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYCREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            13570..15708
                     /gene="nrdD"
                     /locus_tag="IU325_06630"
     CDS             13570..15708
                     /gene="nrdD"
                     /locus_tag="IU325_06630"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP5808716.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWSIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            15868..16332
                     /gene="nrdG"
                     /locus_tag="IU325_06635"
     CDS             15868..16332
                     /gene="nrdG"
                     /locus_tag="IU325_06635"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HAP5808717.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPDKDIW
                     VWTGYKLDDLNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(16377..16763)
                     /gene="cybC"
                     /locus_tag="IU325_06640"
     CDS             complement(16377..16763)
                     /gene="cybC"
                     /locus_tag="IU325_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HAP5808718.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDTLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(16818..18170)
                     /gene="pmbA"
                     /locus_tag="IU325_06645"
     CDS             complement(16818..18170)
                     /gene="pmbA"
                     /locus_tag="IU325_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HAP5808719.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFEAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGAVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            18264..18815
                     /locus_tag="IU325_06650"
     CDS             18264..18815
                     /locus_tag="IU325_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HAP5808720.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(18971..20344)
                     /gene="mpl"
                     /locus_tag="IU325_06655"
     CDS             complement(18971..20344)
                     /gene="mpl"
                     /locus_tag="IU325_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HAP5808721.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESNFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            20520..21518
                     /locus_tag="IU325_06660"
     CDS             20520..21518
                     /locus_tag="IU325_06660"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HAP5808722.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(21551..22546)
                     /gene="yjfF"
                     /locus_tag="IU325_06665"
     CDS             complement(21551..22546)
                     /gene="yjfF"
                     /locus_tag="IU325_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HAP5808723.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(22533..23555)
                     /locus_tag="IU325_06670"
     CDS             complement(22533..23555)
                     /locus_tag="IU325_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP5808724.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLILFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(23569..25071)
                     /locus_tag="IU325_06675"
     CDS             complement(23569..25071)
                     /locus_tag="IU325_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP5808725.1"
                     /translation="MTTNQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFDLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETCELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(25211..26167)
                     /gene="ytfQ"
                     /locus_tag="IU325_06680"
     CDS             complement(25211..26167)
                     /gene="ytfQ"
                     /locus_tag="IU325_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HAP5808726.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            26477..27007
                     /locus_tag="IU325_06685"
     CDS             26477..27007
                     /locus_tag="IU325_06685"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HAP5808727.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(27387..27728)
                     /locus_tag="IU325_06690"
     CDS             complement(27387..27728)
                     /locus_tag="IU325_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAP5808728.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(27731..31510)
                     /gene="tamB"
                     /locus_tag="IU325_06695"
     CDS             complement(27731..31510)
                     /gene="tamB"
                     /locus_tag="IU325_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HAP5808729.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQIYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(31507..33240)
                     /gene="tamA"
                     /locus_tag="IU325_06700"
     CDS             complement(31507..33240)
                     /gene="tamA"
                     /locus_tag="IU325_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HAP5808730.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGVSGQLEKNVRAQLS
                     TIESDEVAPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            33446..34084
                     /gene="msrA"
                     /locus_tag="IU325_06705"
     CDS             33446..34084
                     /gene="msrA"
                     /locus_tag="IU325_06705"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP5808731.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            34407..35750
                     /locus_tag="IU325_06710"
     CDS             34407..35750
                     /locus_tag="IU325_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF21 domain-containing protein"
                     /protein_id="HAP5808732.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(35812..36018)
                     /locus_tag="IU325_06715"
     CDS             complement(35812..36018)
                     /locus_tag="IU325_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 family protein"
                     /protein_id="HAP5808733.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            36343..36897
                     /locus_tag="IU325_06720"
     CDS             36343..36897
                     /locus_tag="IU325_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAP5808734.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMGLLQKLLK"
     gene            complement(36987..37898)
                     /locus_tag="IU325_06725"
     CDS             complement(36987..37898)
                     /locus_tag="IU325_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808735.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTYSNNFYNNIQHAFNKKDWI
                     SNYDSLLTLREFFRCATQIDKNSYQVLSSKNETVNAMDKFLISFSLKDNGAEYIMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIPERIKGYIRQ
                     AFGDVSLGYAVLDDIVSKLKRGKFDLQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     DITEEKICEKYKSTIEAFKKKQLALPV"
BASE COUNT         9261 a        10095 c         9337 g         9246 t
ORIGIN      
        1 tatttactgc gttaaggtca tctatttctt tcagtatccc tttttcacca agcaagtatg
       61 aaatcgattc attacataca taaggcatta atagcagatg ttctttcctt attgctaata
      121 atgcattatt caatggtttt tctaataaat ccgtaatcgt agaatcaact gaatctttac
      181 aactcacctg cgagttattc aagtcctgag aattatttaa ctcctgcgga atatttaata
      241 gacgagctat ttccttgtta gcttcgcgtt ggtaacacgc cagaatcttg ttagtaatca
      301 ggtgtaatga actctcatct tttctttcaa aggacttccc ttcataacga ttagtcgaac
      361 catcaggttg tgtaaatata atgactgtat gtccttcacg aatcccatta ttaaaagctg
      421 aggcaagtgt aatttctatt tccaccttag ataaatccgc tcgctcccct tcaggattat
      481 tcataacaaa cttcattatc tcactaccat cattattttt gatataagat gaggacttaa
      541 tatgagccat catatttcta cccgcttcgt tggagcgttt gctaaaatcc acataaccca
      601 gggtaaatat cttagccaaa aactccaaaa atgctctggg agaaagaata ttgcttctgc
      661 tattctgccc tccggcaatt cgggcaaaat cattcattcc cacataccag gtatctaagt
      721 cttcattttt ttgtttacaa ttgtcggctg atgtaatgtt ttgagaaaaa ttccaacctg
      781 atattttgct catgacggac tccttgagtt aataagaacg tctaagcatt acaaatttta
      841 ctcacaaaaa aatatcaaaa aacgctcttt tcacaaactc gccgtaaacc tggagttatt
      901 tacggcgaaa agtcatcacg ccatcagcat cttcactact gctttgcgaa cccgggctgg
      961 cgcgcccact gcgcacagcg gtttatgcac ttcccccgga taaaacacaa caaaatcacc
     1021 ttcattaagg ataactgttt tctcatcaac accttccggc aaaaatgcga tgtctttatc
     1081 agctaaccag tcggtatccg gcgtacctgc aggttgtgtg ctgaaggtca tgccttcctg
     1141 accttttaac acaatctgaa tgtcgagata gcgggcatgg tactccgcac ggcgagcttc
     1201 gtacggctcg gtcatatctt ccgagataag ataaaacaga cgattgcctt cgatatcgtg
     1261 cttgcccttt ggcgtttctg ccgtaacgtg tgctttgata tgctcaatcg cctggcgtaa
     1321 ctcctggggt agccacggct gaagattatg aatatttccg atgatcatct tttccactcc
     1381 ttaattaaaa caacgtttca tttctatcgt tatacgaaaa gatgatcaat tccgctagcc
     1441 ttaacgcgtg aattattgtc ttatcacaac ttatcggcag gcgcgttccc gcctttcgga
     1501 tcaaggccag catcactcct tgagaagagt ttttatcgct tcccccgcca gtgcagctgt
     1561 tgcacagagt tgttcgattg agtgcccctc ccgtgccttc gcagaaagcc ctgacatcac
     1621 ggtactcata aaatcagtca cacattgtgc gctttgtgga tgccgcctgg cgatatagtc
     1681 ataaatggtc gtttctgcgg cgtgataata ttgaacggca atatcacgcg ctagtggatc
     1741 atgactatga ataccttcaa gaaccataca gccagcgcag ccgccgtttt ggctatatct
     1801 gcgcgccgct tcttttaata cctcaaccag gcactcgcct actggacgat catcacgaag
     1861 aatatcggca agcggaatag cttccgtacc gacgtattca ttgagtacac ggctaaataa
     1921 cccagcttta ctgccaaaag ccgcgtagag gctcgggggg ttaataccaa gataatcagt
     1981 aacttcagca acactgacag catcgaaacc tttttggtga aaaagcactt ttgccgcaga
     2041 gattgcctgc tcaggagcga aacgtctggg acgacctgga acgcgagatt gttttttagt
     2101 gaccattaca aaacctgttg acagaaagtt aaaacagttt tgtaatgcat gttacataat
     2161 aaatcaagga gtccttatgg gcgcttttac aggtaagaca gttctcatcc tcggtggcag
     2221 tcgtggtatc ggtgccgcta tcgtacgtcg tttcgtcacc gatggggcca atgtacgatt
     2281 cacctatgcg gggtcgaaag atgccgctaa acgcctggca caagagactg gagcgacagc
     2341 agtattcaca gatagtgctg acagagacgc tgtcattgat gtcgttcgta agagcggcgc
     2401 attggatatc ctggtggtaa atgcaggtat tggcgtcttt ggcgaggccc tggaattaaa
     2461 tgccgacgat attgatcgcc ttttcaaaat caatattcat gctccttatc atgcctctgt
     2521 tgaagccgcc cggcagatgc ccgaaggcgg gcgcatctta atcatcggct ccgtgaatgg
     2581 cgatcgtatg cctgttgcag gcatggctgc ttatgccgcc agcaaatctg ccctgcaagg
     2641 catggcgcgc gggctggccc gtgattttgg accgcgtggg atcaccatta acgtcgtcca
     2701 gccagggcca attgataccg acgcaaatcc cgccaacggg ccaatgcgcg atatgttgca
     2761 tagtttgatg gctatcaaaa gacatgggca accggaagag gtcgctggta tggtcgcatg
     2821 gttagcaggg ccagaagcca gttttgttac cggcgcgatg cataccattg atggcgcgtt
     2881 tggcgcataa ccgactacgc tcaattaagc ccagccatta tccatgatgt ctgggttttg
     2941 tttactcacg tcgtccgcta aaagcggctc ccggtaaaga gaaatcttct gttcatgtcg
     3001 taaatcttga ggataaccaa atggtagaaa gaaccgctgt tttccctgct ggccgacatt
     3061 cactatatgc tgagcatcgt tattctgcgg ctattcgttc cggcgatttg ctgtttgttt
     3121 ccgggcaagt aggaagtcga gaggacggaa caccagaacc cgattttcag caacaagtca
     3181 gactggcatt tgataatttg catgcgaccc tggcagctgc gggatgcact tttgacgata
     3241 tcattgatgt tacgagcttc cataccgatc cagaaaacca atttgaagac atcatgacgg
     3301 tgaaaaatga aatatttagc gccccacctt atccaaactg gacggcggtg ggtgttacat
     3361 ggctggcagg ctttgatttt gaaattaaag tgatagcgcg catccctgag cagtaagcaa
     3421 tagtgttagc cgttcgcttt cacactccgc cctataagtc ggatgaatgg aataaaatgc
     3481 atatctgatt gcgtgaaagt gaaaaaggaa aaagcaggga atgtctgcaa ttattgatac
     3541 cgaaggacag ttcccctgca gaatcacatc aaataaaaat gcatatacct tgacttttaa
     3601 ttcaaataaa ccgtttgcgc tgacaaaata ttgcatcaaa tgcttgcgcc gcttctgacg
     3661 atgagtataa tgccggacaa tttgccggga ggatgtatgg ttcagtgtgt tcgacatttt
     3721 gtcttaccgc gtctgaaaaa agacgctggc ctgccgtttt tcttcccgtt gatcacccat
     3781 tcccagcccc tcaatcgagg ggcttttttt tgcccaggcg tcaggagata aaagatggct
     3841 aatccgctat atcagaaaca tatcatttcc ataaacgacc ttagtcgcga tgaccttaat
     3901 ctggtgctgg cgacagcggc gaaactgaaa gcaaacccgc aaccagagct gttgaagcac
     3961 aaagtcattg ccagctgttt cttcgaagcc tctacccgta cccgcctctc tttcgaaaca
     4021 tctatgcacc gcctgggggc cagcgtggtg ggcttctccg acagcgccaa tacatcactg
     4081 ggtaaaaagg gcgaaacgct ggccgatacc atttcggtta tcagcactta cgtcgatgcg
     4141 atagtgatgc gtcatccgca ggaaggtgcg gcgcgcctgg ccaccgagtt ttccggcaat
     4201 gtaccggtac tgaatgccgg tgatggctcc aaccaacatc cgacgcaaac cttgctggac
     4261 ttattcacta ttcaggaaac ccaggggcgt ctggacaatc tccacgtcgc aatggttggt
     4321 gacctgaaat atggccgcac cgttcactcc ctgactcagg cgttagcgaa gttcgacggc
     4381 aaccgttttt acttcatcgc gccggacgcg ctggcaatgc cgcaatacat tctggatatg
     4441 ctcgatgaaa aagggatcgc atggagtctg cacagctcta ttgaagaagt gatggcggaa
     4501 gtagacatcc tgtacatgac ccgcgtgcaa aaagagcgtc tggacccgtc cgagtacgcc
     4561 aacgtgaaag cgcagtttgt tcttcgcgcc agcgatctcc acaacgccaa agccaatatg
     4621 aaagtgctgc atccgctgcc gcgtgttgat gagattgcga cggatgttga taaaacgcca
     4681 cacgcctggt acttccagca ggcaggcaac gggattttcg ctcgccaggc gttactggca
     4741 ctggttctga atcgcgatct ggtactgtaa ggggaaatag agatgacaca cgataataaa
     4801 ttgcaggttg aagctattaa acgcggcacg gtaattgacc atatccccgc ccagatcggt
     4861 tttaagctgt tgagtctgtt caagctgacc gaaacggatc agcggatcac cattggtctg
     4921 aacctgcctt ctggcgagat gggccgcaaa gatctgatca aaatcgaaaa tacctttttg
     4981 agtgaagatc aggtagatca actggcattg tatgcaccgc aagccacggt taaccgtatc
     5041 gacaactatg aagtggtggg taaatcgcgc cccagtctgc cggagcgcat cgacaacgtg
     5101 ctggtctgcc cgaacagcaa ctgtatcagc catgccgaac cggtttcttc cagctttgcc
     5161 gtgcgaaaac gcgccaatga catcgcgctc aaatgcaaat actgtgaaaa agagttttcc
     5221 cataatgtgg tgctggccaa ttaattgcgg ttggtaataa aagtctggct ccctataatg
     5281 agccagactt tttaccgctg taataaagga gaaatcatga gcaaaactat cgcgacggaa
     5341 aatgcaccgg cagctatcgg tccttacgta cagggcgttg atctgggcaa tatgatcatc
     5401 acctccggtc agatcccggt aaatccgaaa acgggtgaag taccggcaga cgtcgctgca
     5461 caggcacgtc agtcgctgga taacgtaaaa gcgattgtcg aagccgctgg cctgaaagtg
     5521 ggcgatatcg ttaaaaccac cgtgtttgtg aaagatctga acgacttcgc aaccgtaaac
     5581 gccacttacg aagccttctt caccgaacac aacgccacct tcccggcacg ttcttgcgtt
     5641 gaagttgccc gtctgccgaa agacgtaaaa attgagatcg aagcgatcgc tgttcgtcgc
     5701 taatcttgat ggaaatccgg gctatcatgc ccggattaag tctgatgaca aacgcaaaat
     5761 cgcctgatgc gctacgctta tcaggcctac gtgattcctg caatttattg aatttgttgg
     5821 ccggataagg catttacgcc gcatccggca tgaacaaagc ttactttgtc tacaatctga
     5881 atcggggcta tcgtgcccag tttattcttt attgccagcc gtaacgacgg ctataaaatc
     5941 ctttcaccaa ctgggttaac gtcatatacc ctgccagaat cgcaaccagc cacgggaaat
     6001 agcttaacgg cagcgcctgc agctgcagat aactggccag cggtgaaaac ggcaatgcga
     6061 tcccgacaat catcacgatc acggtcatga tcattaacgg ccacgatgca cagctctgaa
     6121 taaacggcac acggcgggta cggatcatat gcacaatcag cgtttgcgac agtaagccca
     6181 ccacaaacca tcccgactgg aacagcgttt gcgtttccgg cgtgttggca tggaataccc
     6241 accacatcag gcaaaacgtc agaatatcga agatcgagct gatcggtccg aagaagatca
     6301 taaagcgccc cagatccgcc ggattccaac gttgcggctt ttgaatttgc tcgtcgtcga
     6361 cgttatcaaa cgggatcgcc acctgtgaca catcgtacag caggttctga ataagcaagt
     6421 gtaacggcaa catcggcagg aagggcaaga aagcactcgc taccagcacg ctgaacacat
     6481 taccgaagtt agagctcgcc gtcattttga tgtatttcag catgttggcg aaagtgcgac
     6541 gtccctcaat aaccccctct tccagcacca tcaggctttt ttccagcagg atgatatcag
     6601 ccgcttcacg ggctatatct accgcgccat ccacagaaat gccgatatcc gcagcgcgta
     6661 aggcgggcgc atcattaata ccatcgccca taaagccaac cacatgccct tcgcgcttca
     6721 gtaaggtcac aatgcgttct ttatgcatcg gcgtcagacg agcaaacagc gtggtgcgct
     6781 gtgcgagatt tgccagttcg tcgtcagata gcgtttcaat atcactacca atgaccacct
     6841 ctcccgcgtc cagccccact tcatggcaca ctttcgctgc aactaactca ctgtcgccag
     6901 taaggatttt tacggtaatt ccactcgctt ttaatgcctt cagtgccgga gcagttgtct
     6961 cttttggcgg atcaaggaaa gcaatatatc cttcgaggat caggtcggat tcatccgccc
     7021 gctggtaatc tccttcacgc gctggcaggt atttcgtcgc caccgcaacc acgcgcagcc
     7081 cctgacgatt cagcgtatca gtaacccgct taatcttacg cagcatgatg tcatcgagcg
     7141 gcacaatctc gccattgtga cgcacctgcg aacacacatt gaggatttcc tgcaatgcac
     7201 ctttgcaaac cagctgatgg tgctcggtat tttctgccac taccaccgac atccggcgac
     7261 gctcgaaatc aaacggaatc tcatcgattt tttgccaacg actggccagc gagcgcgctg
     7321 actcttcatc cgtaccttcg agcaccgctg tatcgagcag gtttttaagt ccggtctgat
     7381 aatgactgtt caaccacgcg ctatgcagca cgcgttcgct ggttttaccg gagatatcgg
     7441 tatgattctc cagcacaatt ttgtcctgtg tcagggtgcc agttttatca gtgcacagaa
     7501 tatccattgc gccaaagttc tgaatagcat ccagatgttt gacgatcact ttctgtttcg
     7561 acagctttac tgcgccgcgc gccagcgtcg aggtaacaat catcggcaac atttccggcg
     7621 ttaagcctac cgcgaccgaa agcgcaaaca gtgccgcttc ccaccagtcg cctttggtgt
     7681 aaccattgat taacagcacc accggagcca tcaccagcat aaagcgaatc agcagcatac
     7741 tgacgcggct gatcccttgc tgaaaggcat tcggctcgct ttcctgctca ctaacacgcc
     7801 ccgccagttg accaaaccag gtattggcac ctgtagcaat caccattgct tgtgccgtgc
     7861 cgctcaccac ggtggtgccc ataaaacaca gggtgtcgca ctccagcgga ttgctgtgct
     7921 ccggctggcg agtggtagct gctttttcta cgggcagaga ctcaccggtt aacgacgcct
     7981 gagcgacgaa cagatcccgc gcctgcaaga tacgtaaatc tgccgggatc atatcccccg
     8041 ccgccagttt tataatatcg ccgggcacca gctggtcgat cgggatctcc agccagccat
     8101 tttcgccttt gtcgttaatt acgcgcagca ccgtcgcagt attgctgacc atcgctttca
     8161 gggcatctgc cgctttagtg gaacgtgctt cctgaataaa gttcagcaac gtagaaatag
     8221 cgaccattag cgcgataacg cccgcggcaa ataaatcttc cgtggcgtaa gaaatagcgc
     8281 cgagaatggt gagtaaaata ttaaatgggt tgcgatagca gacccataaa tgtacccacc
     8341 acggcgacgg ttgttgtgcg ggtaatttat tttcaccatg ttgttcgcgg gcagattcca
     8401 cttccgcctg atttaaccct tccggatggg tgtcgaacgt tttccataat tcttcttcgg
     8461 gcatcaccgc cattttcagg caatgcgcac ttaaggacgg cgggaccgcc gtattcactg
     8521 tctgctgcgc gccaggcaat ggatcgcgat gaaccagacg ggaaggtaaa tggcgaatga
     8581 gccgggtaaa aatttcttta aacataagga gtccctccgc actgtctgaa acagtacaga
     8641 aatgtcttca ggcgtggcga agccttacct gaaggtaata aagctgagca gggacgttaa
     8701 aacgtcacgg ccttacgcat ccggtaacgc cgtgaaaaga cgggcttacc ggaaaagttt
     8761 taatctccgt cgagggagag gcgtgggatc aggttccata taacctccgg taagtgaaaa
     8821 aagcgccgca aataataagg agaatccaat aatattcgta gcatctatgg cgatataata
     8881 cctgctggca actaaaccaa cgataaacca gactttaccc attgctgaat gcacgggtaa
     8941 agttaggctc aaataattaa acaacacgtt acaggacaac aggatgcaaa atcggctgac
     9001 catcaaagac atcgcacgct taagcggcgt ggggaaatct acagtttccc gggtgctgaa
     9061 taacgaaagc ggcgtgagcc agcgcacccg cgagcgtgtt gaagcagtga tgaatcagca
     9121 tggattttcc ccttcccgct ctgcgcgcgc tatgcgtggg caaagcgata aagtggtcgc
     9181 catcattgtt acccgtctgg attcgttgtc agaaaatctc gccgttcaaa ccatgctgcc
     9241 agcgttctat gaacaaggtt acgacccaat catgatggaa agtcagtttt ccccacaatt
     9301 agttgccgaa catttggggg tgctgaaacg gcgtaatatc gacggcgtag tgctgttcgg
     9361 ttttactggc ataacagaag aaatgttagc ccactggcag tcatcgctgg ttctgctggc
     9421 gcgtgacgca aaaggctttg cttcggtctg ttatgacgac gaaggggcaa tcaagatcct
     9481 gatgcaacgg ctgtatgacc aggggcatcg taatatcagt tatctcggcg tgccgcatag
     9541 tgacgtgaca accggtaagc gacgtcacga agcctacctg gcgttctgca aagcgcataa
     9601 actgcatccc gttgccgctc tgccagggct tgctatgaag caaggctatg agaacgtggc
     9661 aaaagtgatt acgcctgaaa ctactgcgtt actgtgcgca accgacacgc tggcacttgg
     9721 cgcaagtaaa tacctgcaag agcaacgcat cgacaccttg caactggcga gcgtcggtaa
     9781 tacaccgtta atgaaattcc tccatccgga gatcgtcacc gtagatcccg gttacgccga
     9841 agctggacgc caggcggcct gccaattgat cgcacaggta accgggcgca gcgaaccgca
     9901 acaaatcatc atccccgcca ccctgtcctg atcgtttcct gaacgataaa ttgtgatctt
     9961 cgctgcgttt cgggaacgtt cccgttttta aatttttccg cgcaatatat tctgcagcca
    10021 accaaaaatg tcatctgcca tggggcttta tgatgagcaa aataaaccaa acggatatcg
    10081 atcggttgat tgaactggtc ggcgggcgcg gcaatattgc gacagtgagc cactgtatta
    10141 ctcgcctgcg ctttgtcctc aaccaaccgg ccaatgccag accgaaagaa attgagcaac
    10201 tccccatggt gaaaggctgt ttcaccaatg ccgggcaatt tcaggtggtg attggcacca
    10261 atgtgggtga ttactatcaa gcactaatag cgtcaaccgg acaggcgcag gttgataaag
    10321 agcaggtaaa aaaagccgcc cggcagaata tgaaatggca tgagcagttg atctctcatt
    10381 tcgcggagat cttcttcccg ttgctgcccg cgttgattag cggcggtttg atcctcggtt
    10441 ttcgcaatgt gatcggcgat ttgcccatga gcaacggtca aacgctggcg caaatgtacc
    10501 cttccctgca aacgatctac gattttctgt ggttgatcgg tgaagcgatc ttcttctacc
    10561 tgccggtcgg gatttgctgg tcagcggtga aaaaaatggg cggcacgccg atcctcggta
    10621 tcgtgcttgg cgtgacactg gtttctccac agctgatgaa cgcttatctg ctcgggcagc
    10681 agctgccgga agtgtgggac tttggcatgt tcagcatcgc caaagtaggc tatcaggcgc
    10741 aggtgatccc ggcactgtta gccggactgg cgctgggcgt tattgaaact cgccttaaac
    10801 gcatcgtgcc ggattacctc tatctggtgg tggtacccgt ctgttcgctg atcctcgcgg
    10861 tgttcctcgc ccatgcgctg attggtccgt ttggtcgcat gattggcgat ggcgttgcct
    10921 ttgcggtacg tcacctgatg accggcagct ttgctccgat tggtgcggca ttgtttggct
    10981 tcctgtacgc gccgctggtg atcaccggcg tacaccagac cacccttgct attgatttgc
    11041 agatgattca aagcatgggt ggcacgccag tgtggccgct gattgcgctg tcgaatatcg
    11101 ctcagggctc cgccgtgata ggcattatca tttccagccg caagcacaat gaacgcgaga
    11161 tctccgtgcc tgccgctatc tccgcctggc ttggggtcac tgagcctgca atgtacggca
    11221 tcaacctgaa atatcgcttc ccgatgctgt gcgcgatgat tggttctggt ctggcaggat
    11281 tgctatgcgg cctgaacggc gttatggcga atggcatcgg cgtaggcggc ctgccgggaa
    11341 ttctctcgat tcaaccgagc tactggcagg tgtttgcgct ggcaatggct atcgccatca
    11401 tcatcccgat tgtactcacc tcgtttatct atcagcggaa ataccgcctg ggcacgctgg
    11461 acattgttta attttcttcg gggcgcaatt gcgccctctc gcatttgcag gaataacgta
    11521 atgactaatc ttccccactg gtggcaaaac ggcgttatct accagattta tccaaagagt
    11581 tttcaggaca ccacgggtag cggcaccggc gatttacgtg gcgttatcca acgcctgggc
    11641 tatctgcata aactgggcgt tgatgccatc tggctaaccc ccttttatgt ctctccccag
    11701 gtcgataacg gttacgacgt agcgaactat acggcgattg atcccactta cggcacgctg
    11761 gacgattttg acgaactggt gacgcaggca aaatcgcgcg ggattcgtat cattctcgat
    11821 atggtgttta accatacctc tacccaacat gcctggtttc gcgaggcgct gaacaaagaa
    11881 agcccttacc gccagtttta tatctggcgc gatggcgaac cagaaacgcc accgaacaac
    11941 tggcgttcaa aatttggcgg tagtgcgtgg cgctggcatg cggaaagcga acagtactat
    12001 ttgcatctct ttgcaccaga gcaggcggat ctcaactggg agaatccagc ggtacgcgca
    12061 gagctgaaaa aagtctgtga gttctgggcc gatcgtgggg tcgacgggtt gcgcctggat
    12121 gtggtgaatc tgatctccaa agacccacgt ttccctgatg acctggatgg cgacgggcgt
    12181 cgcttctaca ccgacgggcc acgagcacac gagtttttgc acgagatgaa ccgcgatgtg
    12241 tttacgccac gcgggttaat gaccgtaggt gaaatgtcct ccaccagcct tgagcattgc
    12301 cagcgatacg cggcactgac aggcagtgaa ttgtcgatga cctttaattt tcatcacctg
    12361 aaggtcgatt atcccggtgg tgaaaaatgg acgctggcta aacctgactt tgtggcgttg
    12421 aaaacattgt tccgccactg gcaacaagga atgcacaacg tggcatggaa tgccttgttc
    12481 tggtgtaacc acgatcagcc gcgcattatt tctcgctttg gtgatgaagg tgaataccgc
    12541 gtgcctgcgg caaaaatgct ggcgatggtg ctgcatggca tgcagggaac gccgtatatc
    12601 taccagggcg aagagattgg catgaccaac ccgcatttca cgcgcattac tgactatcgc
    12661 gacgtggaga gcctcaatat gtttgccgag ctgcgcaacg atggtcgtga tgccgacgag
    12721 ttattggcaa tcctcgccag taaatcccgt gacaacagcc gcacgcccat gcaatggagc
    12781 aacggcgata atgccgggtt tacggctggc gaaccgtgga ttggcctggg cgataactat
    12841 caacaaatca acgtagaagc cgcgctggcc gatgagtcct cggtgtttta cacctaccaa
    12901 aagttaatcg cactgcgtaa gcaagaagcc atcctgacat ggggcaatta ccaggatctg
    12961 ctgccaaaca gccctgtatt gtggtgctat tgccgtgaat ggaaggggca aaccttgctg
    13021 gtcattgcca accttagccg tgagatccaa ccctggcagc cagggcaaat gcgcggcaac
    13081 tggcagcttg tgatgcataa ctacgaagaa gcctcaccac aaccctgtgc catgaattta
    13141 cggccttttg aggctgtctg gtggttacag aagtaaatct tccctaagcc ccggtaatgc
    13201 cggtcattcc ggggttttgt ctgtcagcat gttgtttttg ttgatttttc aaccagcaaa
    13261 ttcattaaaa aatttacata acgctgtagc gcccgtcatc cgtacgctct gctttttact
    13321 ttgagctaca tcaaaaaaag ttaaaacatc cttgatgcaa agcactatat atagacttta
    13381 aaatgcgtcc caacccaata tgttgtatta atcgactata attgctacta cagctcccca
    13441 cgaaaaaggt gcggcgttgt ggataagcgg atggcgattg cggaaagcac cggaaaacga
    13501 aacgaaaaaa ccggaaaacg cctttcccaa tttctgtgga taacctgttc ttaaaaatat
    13561 ggagcgatca tgacaccgca tgtgatgaaa cgagacggct gcaaagtgcc gtttaaatca
    13621 gagcgcatca aagaagcgat tctgcgtgca gctaaagcag cggaagtcga tgatgccgat
    13681 tattgcgcca ctgttgccgc ggttgtcagc gagcagatgc agggccgcaa ccaggtggat
    13741 atcaacgaga tccagaccgc agttgaaaac cagctgatgt cgggtccata caaacaactg
    13801 gctcgcgctt acatcgagta ccgtcacgat cgcgacattg aacgtgaaaa acgcggtcgc
    13861 ctgaaccagg agatccgtgg tctggtcgag cagaccaacg cctcgttact caacgaaaac
    13921 gccaacaaag acagcaaggt gattccaacc cagcgcgacc tgctggccgg gatcgtggct
    13981 aaacactatg cacgtcagca cctgctgccg cgtgacgtgg tgcaggcaca tgagcgtggc
    14041 gatattcact atcacgatct cgattactca ccgtttttcc cgatgttcaa ctgcatgttg
    14101 atcgacctga aaggcatgct gacccagggc tttaaaatgg ggaacgccga gattgaaccg
    14161 ccgaagtcga tctcaacggc aaccgcggta actgcgcaga ttattgctca ggttgccagc
    14221 catatttatg gcggcaccac cattaaccgt atcgatgaag tgctggcacc gtttgtcacc
    14281 gccagctaca acaaacatcg caagaccgca gaagagtgga gcatcccgga cgcagaaggc
    14341 tacgctaact ctcgtaccat taaagagtgc tacgatgcct tccagtcgct ggagtacgaa
    14401 gtaaacaccc tgcataccgc caacggtcaa acgccgtttg taacttttgg ttttggtctg
    14461 ggcaccagct gggaatcgcg cctgattcag gaatctatcc tgcgtaaccg tatcgcaggt
    14521 ctcggtaaaa accgtaaaac tgcggtgttc ccgaaactgg tgtttgcgat tcgcgatggt
    14581 ctgaaccata aaaaaggcga tccgaactac gacatcaaac agctggcgct ggagtgcgca
    14641 agcaagcgca tgtatccgga tatcctgaac tacgatcagg tagtgaaagt caccggttcg
    14701 tttaaaaccc cgatgggctg ccgcagcttc ctcggcgtgt gggaaaatga aaacggcgag
    14761 cagatccacg atggtcgtaa caacctcggc gtgatcagcc tgaacctgcc gcgtattgct
    14821 ctggaagcaa aaggcgatga agccaccttc tggaagctgc tggatgaacg tctggtgctg
    14881 gcacgtaagg cgctgatgac ccgtatcgct cgtctggaag gcgtgaaagc gcgcgtggcc
    14941 ccgatccttt atatggaagg tgcttgtggc gtgcgtctga atgctgacga tgatgtttct
    15001 gaaatcttca aaaacggtcg tgcgtctatt tcgctgggtt acatcggcat ccacgaaacc
    15061 attaacgcgc tgttcggcgg cgagcatgtt tacgacaacg agcagctccg cgcgaaaggt
    15121 atcgcgattg ttgaacgtct gcgtcaggca gtggatcagt ggaaagaaga aacgggttat
    15181 ggtttcagtc tctacagcac gccgagtgaa aacctgtgcg accgcttctg ccgtctcgat
    15241 actgctgagt ttggcgtggt gccgggcgtg accgataaag gttactacac caacagtttc
    15301 caccttgatg tggagaagaa ggttaacccg tacgacaaga tcgactttga agcgccttac
    15361 ccgccgctgg cgaacggtgg tttcatttgc tacggcgagt atccaaacat tcagcacaac
    15421 ctgaaagcgc tggaagatgt ctgggattac agctatcagc atgtaccgta ttacggcacc
    15481 aatacaccga ttgatgagtg ctacgagtgt ggctttaccg gtgagttcga gtgcaccagc
    15541 aaaggcttta cttgcccgaa atgtggtaac catgacgcct cccgtgtgtc ggtaactcgc
    15601 cgcgtgtgcg gatatttagg tagcccggat gcacgtccgt ttaacgctgg taagcaggaa
    15661 gaagttaagc gccgcgttaa acatttgggg aatgggcaga taggttaatc ttcactctgc
    15721 gctaagcaaa tgccggatgc ggcgtaaacg ccttatccgg cctacatttg gcaacggctg
    15781 tgtaggcctg ataagacgcg acagcgtcgc atcaggcatt gattaccgga tgcagcctac
    15841 acgcggactg ataagccagg cgaaataatg aattatcatc agtactatcc tgtcgacatc
    15901 gtcaacggcc ccggcactcg ctgcaccctg tttgtctccg ggtgtgttca tgaatgcccc
    15961 ggttgctata acaaaagcac atggcgggta aattctggtc agccatttac caaagcaatg
    16021 gaagaccaga tcattaacga tctgaatgac actcgtatca aacgccaggg gatttccctc
    16081 tccggcggcg atccgctgca tccgcaaaac gtgccggata ttctgaaact ggtaaaacgc
    16141 atccgcgccg agtgtccgga taaagacatc tgggtgtgga caggctataa actcgacgat
    16201 ctcaacgctg cgcaaatgca ggttgttgat ttgattaacg tactggtcga cggcaaattt
    16261 gtgcaggatt taaaagaccc ttccctgatc tggcgcggca gcagcaatca ggtggtgcat
    16321 catttgcgat gataagttcc ctgttacctg agtgcaggca acagggaaat gaggaattaa
    16381 cgatacttct ggtgataggc gttgcgggtc gttttcagtt gctctgcggc agcctgcgct
    16441 tcttttactt taccttcatt tgccagcttc agcgcgtcgt caatctgacc aaccagaata
    16501 tcgaaaccgt gacggaagtc tttcatttcc gggctgtccg gtgatttatc ttcgagcttc
    16561 ggcggcgttg ctttttgcgc atccagcgct gcggcgcgca tcttcgttaa cgcgtctttg
    16621 acttgcgccg cgttatcggc tttttcgacc acttttaaag tgtcgttgag ggtttccata
    16681 ttgtcttcaa gatcagcggc aaacgacgcc gaactgaata ccaacgagga gactgcaaga
    16741 atagctaaca ggcttttacg cattgctcac ttcctttttt attatgaaga tttggcgcgg
    16801 caggtcgcgc cagtttttta ctgcccggcg attttcatct ccggcaacag cacagaacca
    16861 cactgtatat tactgcgtgt ttcaatatcg ttaccgacgg tgacaatatt gcgccacata
    16921 tcttttaaat tacctgcgat ggtgatttcg ctaaccggat actgaatttc gccgttttct
    16981 acccagaagc ccgccgcacc acgggaataa tcaccggtaa ttgcactcac gccctggccc
    17041 atcagctccg tcaccaccag cccggtgccc atctctttga gcatttgttc gaagcttagc
    17101 ccttgtccgg caatccgcca gttgtgaata ccgcccgcat gtccggtgct tttcagcccc
    17161 agttttcgcg ccgaatagct ggtcagcagc cactgagtca ggatgccatc tttaataata
    17221 tcgcgacgct cggtgcgcac accttcgctg tcaaatggcg tcgatgccag ccctttcagc
    17281 agatgcggat gctcttcaat ggtcagccag tccggcagaa tttgtttacc cagcgaatcc
    17341 agcaggaagg tagatttacg gtaaaccgct ccacccgcta tcgcccccac cagatgccca
    17401 aaaagcccgg ttgccacttc attggcaaaa atgaccggcg ctttcatggt ggagagttta
    17461 cgcggtgaca ggcgcgataa agtacggcga gcacagtcgg ccccaaccca ctctggcgtt
    17521 tgcagatcgc tcatcgcacg accaatggtg taggcgtaat cacgctccat atcgccattt
    17581 tcttcggcaa ttacacagct ggagagcgaa tgacgcgtag agcagtaacc ctgcaacatg
    17641 ccgtggctgt tgccaaaaac tttgacaccg tagtggctgt taaagctgcc accttcggta
    17701 ttggtgatac gtttgtccgc ctgtaatgct gcctgttctg cgcgggccgc cagttcaatg
    17761 gcttcatccg gggaaacttc cgcagggtgg aacaaatcga gatccggtgc ctcaaaggcc
    17821 agcagctctt tgtctgccac gccggcacag ggatctggcg aggtataacg ggcaatatcc
    17881 agcgccgcct gtacggtgcg ggcaatggcc tgcgggctta aatcggtgga tgatgcgcta
    17941 cctttgcggt tctggtgata aacagtgatc cccagcgcgc catcgctatt gaattcaaca
    18001 ttctccactt caccataacg cgtgcttacg ctaatgccgg tggtcttgct gacggcaact
    18061 tccgcaccgt ccgatttgcc tgaggccaac tccagcgcag tggaaactgc ttcttccaga
    18121 atcttgcgct gcgcttcaac ttgagagatt actttcattg caagtgccat aatgtagaga
    18181 gtttctctga agtctaacag agaaccgttt ttcagtgcgc atcttaactg gtaacattag
    18241 cctctttttt taaggagcct gagatgacta agcagcccga agactggctc gacgacgttc
    18301 ccggtgatga catcgaagac gaagacgatg aaattatctg ggtcagtaaa agtgaaatta
    18361 aacgtgatgc cgaggagcta aaacgccttg gcgcggaaat tgttgatctg gggaaaaacg
    18421 cgctggataa gatcccgtta gatgcggatc tgcgcgcggc tattgagctc gcccagcgta
    18481 ttaagatgga aggtcgccgc cgccagttgc agctcatcgg taagatgctg cgccagcgcg
    18541 acgtagagcc tattcgtcag gcgctggata agctgaaaaa ccgtcacaac cagcaggtgg
    18601 tgctgttcca taaactggaa aacctgcgcg atcgtctgat cgatcaaggt gatgacgcca
    18661 tcgctgaagt attgaatctg tggcccgatg ccgatcgtca gcaactgcgt acactgatcc
    18721 gcaacgcgaa gaaagagaaa gaagggaata agccgccgaa atccgcacgc cagattttcc
    18781 agtatctgcg cgaactggcg gagaatgaag ggtaattttc gttgttgatg tgagtatggt
    18841 ttgccggatg cggtgcacct gtaggcctga taagacgcat caagcgtcgc atcaggcact
    18901 atgctcaaat gccggatgcg gcgtgaacgc cttatccggc ctacgcacac tatatcaggc
    18961 tgaggccgaa ttactgcgca gcttccgcct tcttcgccag accatccagc agtttctgat
    19021 ggatcccacc aaaaccgccg ttgctcatca ccagaatatg gtcgccaggc tgagcggttt
    19081 tcaccaccat atctgccagc gtatccacat cgccactcca gtgtgcaggc tgaacgcagg
    19141 cttctgccac ttctgccacc tgccacggaa tatgcgccgg ttgcagcagg aagacttcat
    19201 cggcacgacc taatgaaggt gccagatcgt ctttgcagat ccccattttc atggtattcg
    19261 agcgcggttc cagcacagca ataatgcgcg ccgtaccacc aactttgcca cgcagcgccg
    19321 ccagcgttgc cagaatcgcc gtcgggtgat gggcaaaatc gtcatatacc gtgacgccat
    19381 tcgcttcacc acgcaactcc agacggcgac gagcattaat aaacgaaccc agcgcgttag
    19441 cggcatctgc cggcgctaca ccaacatggc gagccgctgc aatcgccatc aggccattgt
    19501 gcatattatg ttcgcctacc agcgaccatt tcacttcgcc cactttttcg ccatccagca
    19561 aaacttccca ttcggaagca tcggtggtca gctttttcgc ctgccagtga ccctgctcgc
    19621 ccaccagctc ctgctcgctc cagcagccca tcgccatggt ctgtttcagg ttgatgtcgt
    19681 tttctggcca gataatacgg ccctgccctg gaacgatacg caccagatgg tggaactgtt
    19741 tctggatcgc tttcaggtcg tcaaagatat cggcgtgatc gaactcaagg ttgttgagga
    19801 tcagcgtacg cgggcagtaa tgaacaaatt tagagcgttt gtcgaagaag gcgcagtcat
    19861 actcatccgc ttcgataaca aagaagttgc tttcgcccag acgcgccgaa acctcaaagt
    19921 tccccggcac accgccgatc acaaatcccg gtttgtaacc gcactgttcc agaatccagg
    19981 tcgccattcc cgcggtggtg gttttgccat gtgtaccggc aacggccagc acccagcggt
    20041 cgcgcagcac aaaatcgtgc agccactgtg gacctgacat ataagggatg tttttttcca
    20101 gtaccgcttc cacacacgga tttccacggg tcatggcgtt gccaataatc accagatccg
    20161 gctgcggatc gagctggctg gcatcgtaac cctgaatcag ttcaatgcct tgcttctcaa
    20221 gtaaggtgct catcggcgga tacacattgg cgtccgaacc cgttacttca tggcctaact
    20281 ggcgcgccag catcgccaga ccgcccataa acgtgccaca aattcctaaa atatgaatgc
    20341 gcatacgtca ctatcctttt ttaatctgcc gctcatttta cgcatatgtc gggcaagtga
    20401 gaaacgcatt tcaggataat ccgcaatttg ctggcgcgat tcacctgagc gcaacattcc
    20461 gattatttgt taagattgtt acggtcgctt tactccataa acattgcagg gaaagtttta
    20521 tgaaaacgtt aggtgaattt attgtcgaaa agcagcacga gttttctcat gctaccggtg
    20581 agctcactgc tttgctgtcg gcaataaaac tgggcgccaa gattatccat cgcgatatca
    20641 acaaagcagg actggttgat atcctgggtg ccagcggtgc tgagaacgtg cagggcgagg
    20701 ttcagcaaaa actcgacttg ttcgctaatg aaaaactgaa agccgcactg aaagcacgcg
    20761 atatcgttgc gggcattgcc tctgaagaag aagatgagat tgtcgtcttt gaaggctgtg
    20821 aacatgcaaa atacgtggtg ctgatggacc cactggatgg ctcgtccaac atcgatgtta
    20881 acgtctctgt cggtaccatt ttctccatct accgccgcgt tacgcccgtc ggcacgccgg
    20941 taacggaaga agatttcctc cagcctggta acaaacaggt tgcggcaggt tacgtggtat
    21001 acggctcctc taccatgctg gtttacacca ccggatgcgg tgttcacgcc tttacttacg
    21061 atccttcgct cggcgttttc tgcctgtgcc aggaacggat gcgcttcccg gagaaaggca
    21121 aaacctactc catcaacgaa ggaaactaca ttaagtttcc gaacggggtg aagaagtaca
    21181 ttaaattctg ccaggaagaa gataaatcca ccaaccgccc gtatacctca cgttatatcg
    21241 gttcactggt cgcggatttc caccgtaacc tgctgaaagg cggtatttat ctctacccaa
    21301 gcaccgccag ccacccggac ggcaaactgc gtttgcttta tgagtgcaac ccgatggcat
    21361 tcctggcgga acaagcgggc ggtaaagcga gcgatggcaa agagcgtatt ctggatatca
    21421 tcccggaaac tctgcaccag cgccgttcgt tctttgtcgg caacgaccat atggttgaag
    21481 atgtcgaacg ctttatccgt gagttcccgg acgcgtaatc tcctgcgcct ctccacacgg
    21541 agaggcgttt ttatcgctgc gcaatgttga ctcttgtcac tggcgaactc tgacgattct
    21601 cccacagcac cgttaatcca cgctgtaatg ctataaaaat aaacaacaaa ataccgatgg
    21661 cgattttcgt ccaccatgag ctcagcgtgc catcaaagtt tatgtaagtc tgaatcagtc
    21721 cctgaatcgc cacgccaaaa agcgtcccta ataccgttcc aacgccaccg ctcagaagcg
    21781 taccgccaat taccactgag gcgatagcat ccagttccac acctacgccc gccagcgcat
    21841 atccggcctg ggtataaatc gagaagacaa tccccgccag cgttgccagt ccggtggaga
    21901 gcatataaat gcgaatagtg gtgctgcgag tggaaatccc catcaggttc gccgacgttg
    21961 cgttgccgcc aatggcgtat acctgattac caaaacgggt acgatgcgcg aggaaaatac
    22021 cgataaccac caccgccaac atcagcagtc ccatcgcact taagcgaccg ccgccaggga
    22081 ttttccacgc aaggcttgag agcgtgtcat aaatgggatg gtttatcgga atcgactctt
    22141 ccgaaacgag atagctgaca ccgcgcaaaa agaacatccc cgccagggta atgataaatg
    22201 ccgggatctt cagggcgtcg atcaaaagcc ccataaatgc gccgaaggca cagcccatca
    22261 ccagcactag cggaaacgcc agtagcggcg agaggccgaa atcgccaatc acttttgcca
    22321 gaaacacgcc agtaaaggcg atcaccgaac cgacggagag atcgatccca ccggagagga
    22381 tcacaaaggt catgccaacg gcaatgatcc caagaaaggc gttatcggtc aggatattgc
    22441 agatcactct tgtggaagca aaaccgggaa actgggtcag gcagtaaaga taacccaaca
    22501 caaagacgcc gatggtgatc atcagtggca aattacgttt tatcacggct acgtactcct
    22561 ttaatcagac tgataaagcg ttgcgactgg acaatcagca cgcaaagaac caccaccgct
    22621 tttacaacct ggttcatctc tggcggaaag cccgaaagca aaattccggt gttcatcccc
    22681 tgaataatca gcgcccccac caccgaaagc agtaggttaa aacgtccgcc catcagcgat
    22741 ccgccgccaa taaccaccgc gagaatggcg tccagctcca gccataaccc ggcgttattg
    22801 gcatcggcac cgcgaatatc cgccgccacg ataatgcccg caatcgccgc acacagcccg
    22861 ctcaacacgt aggtaagcat gacgatgatc cgcgtgttta ccccggcatt ttttgccgcc
    22921 cgaatgttga taccaacggc ttcgataaac atccccagcg ccgttttgcg ggtcaacagc
    22981 cagaacagga taagcgtcag caccgcgata atgaccggcg ttggcaggaa caacagcgat
    23041 ccactgccaa accatgagag atccggcgag ttaaacgtga cgatctgtcc ggaggtgatc
    23101 agttgcgcca cgccgcgccc ggcgaccatc aggatcaggg tggcaacaaa cggctgaatt
    23161 ttgaggatcg ctaccagtat gccgttccac aatcccgcca ggatgccagt gcccagggcg
    23221 cttaacaaaa caatcggcag gctgaatccc gcgaccgtca tcgcagccgt tgtggctccg
    23281 gcgatagcca tcaccgcccc cacggagaga tcaatcccac ctgttgcgat caccagcgtc
    23341 attccgatag ccaacagcgc aacgggggcc gcacggttaa gaatgtctat ggggctaccg
    23401 aataaacgcc catcctggag caccacctgc cagaaatgcg gggccaccag gctatcgacc
    23461 agcagcacca gcaacagtgc cgccagctgc ggcattcccg ttggccagcg aaagcgcctt
    23521 ttcggcggcg tagtgtccgg gagagattga ggcatcacac tgttctcctt acgccgcaat
    23581 ggcgttcatg atcgccggaa cggaaagctc tgccagcggg atctccgcca cctgtttgcg
    23641 atcgcgcatg atgatcaccc ggtcggcata gcccaccagt tcttccagtt cggaggagat
    23701 caccagcagc gccagaccat cggcgcatag cgtttcaatc aggcggatga tctcggcgtg
    23761 ggcaccaaca tcaatgccgc gagttggctc atcaaggatc agaaattgcg gtcgggtcag
    23821 tagccaacgt gaaagcaaca ctttttgctg attgccgcca gagagaaatt caatcggttg
    23881 ttcagttgaa ggtgtgcgaa tgccaagctg gcggataaag cgttcggcaa tctcttgctg
    23941 ttctttgcgg gaaatgggac gtagccagcc gcgctgggcc tggagagcga ggatgatatt
    24001 ttcccgcacc gaggcggcag cgatgatgcc atcggttttc ctgtcttccg ggcagaagcc
    24061 aatgcccagt accgaagcct gatgtggcga tcgcaggttt tgcggtttgc ctttgatcaa
    24121 cgccgtgccg ctgtcagcag gtttgatacc gaagatcact tcggcggttt cggtacgtcc
    24181 tgatcccagc aatccagcca gaccgacgat ctcgccgggg cgtacttcga gatcaaacgg
    24241 tgcgatcgtt ccttttttgc cgtaattttt gaacgcggca acgggtttgt cgctcaacaa
    24301 tgttcgcccg gcacgctgta gcgcgtgggt atccagctcg cgccccagca tcatttttac
    24361 cagttcgatc tgcggtagct cgcacgtttc ccgacagcct acgaaactgc cgttgcgtaa
    24421 gacggtgatc cgatcgctga cctgatagac ctgatcgaga aagtgagtga caaaaatcag
    24481 gctgacgccg cgatcgcgca actgacgcat caggtcaaac agtaactcca cttcctgggt
    24541 gtcgagactg gcggtgggtt catcgaggat cagcactttg gcagagagat cgatagcccg
    24601 gcaaatagcg acgatttgct gcatcgcgac tgaaaagcgg ttgagcggtt cgcgcacgtc
    24661 gagggagaaa ccgtaagatg ccatcagttc ggtggcgcgc ttttccatct ctttgcggcg
    24721 tagaaggccg aagcgtttgg gttcgcggcc tataaataga ttatcagcga ccgacatatt
    24781 gggtagcagg tttacttcct gatagacggt gccgataccg agttgttgtg catgggcggt
    24841 atttttcggt gagatagcct ggccttccag ccagatggtg ccgcgatcgg cgtggtatac
    24901 accagttaat gctttgatta gcgttgattt tcctgccccg ttttcaccga gcagcgccat
    24961 gatttcgccg cggcgcaggc tgaaatcaac gttgtctaac gctttgacgc cggggaaaaa
    25021 tttacttaat ccttcggtgc ggaggatctc ctggtgttgg ttagtcgtca tggtttcccc
    25081 cggtgtaaag cgtgctccca gtaggcatga taagacgcgg taagcgtcgc atcaggcttc
    25141 agcgcaccac cgccggatgc ggcgcgagcg ccttatccgg cctacgaatc ggcatcaggc
    25201 atagcaaccc tcaatacccc atatttttct tcttctctaa ctcttctttt gcggtatcag
    25261 gcagataaag ggtggatttg gtcagcgtca gcttttcagg catggtgccg tcttttttgt
    25321 atttctccag cgcgtcgaag gcgggacctg ccatattcgg cgtcagttca acactggcgt
    25381 tcgcttcgcc atcaatcatc gctttgtaga tatccggtac gccgtcgata gaacctgtca
    25441 gaatatcttt gcctggtttc aggcccgctt ctttaattgc ctgaattgca ccaatcacca
    25501 tgtcgtcgtt atgggcgtaa accatgcaga tgtttttgcc gttgttttcc gctttgataa
    25561 agctctccat gacttctttg cctttactgc gggtgaagtc acctgactgc gagcggataa
    25621 ttttgatatt tggcgcattc ttaatggctt cggcaaagcc tttcttacgg tcaatggcga
    25681 cgctggcccc aacagtgccc tgcagctcca ccacgttgca tggtttgcca ttcacttctt
    25741 ttaccagcca gtcaccaatc aacttgcctt cgagaatgtt gtcggcagtg acggtggtca
    25801 tatagagaga tttgtctttc acatcaatag aacgatcgag caagaagacc gggatttcgg
    25861 catctttcgc ctcttttaat accggttccc aaccagtcgc aaccaccgga gcgataaaga
    25921 tcgcatccac cccttgcgcg acgaaggaac gtaccgcttt aatctggttt tcctgctttt
    25981 gctgaccatc ggcaattttc agcgtaattc cgcgtttttc ggcctcactt ttcgccacat
    26041 tggtttctgc ggcgcgccag ccagattccg atccgacctg cgaaaatcct acggttaatg
    26101 gagcggccaa cgccatagac gacatggctg ccgagactgc agagactaca agtaagcgtt
    26161 tccacatagg gttgtcctcg tcggggtaat tattattggt gaaaagatgt tcgcgaaaaa
    26221 actatagaca attcgttatg taacggattg cgttacatca cacttcaaag aagtaaataa
    26281 aacgttaatc acaagtttgt aatcgctttc atctcactat gaaaaatgcg gctacggtta
    26341 tggattttcc tgctctgtat accgtcttaa aattggcgaa aaaggaaaat gaagacgaaa
    26401 acaagcgaag acattcggcg cgagttggct ataatactcg gcacttgttt gccacatatt
    26461 tttaaaggaa acagacatga gcttactcaa cgtccctgcg ggtaaagatc tgccggaaga
    26521 catctacgtt gttattgaga tcccggctaa cgcagatccg atcaaatacg aaatcgacaa
    26581 agagagcggc gcactgttcg ttgaccgctt catgtccacc gcgatgttct atccgtgcaa
    26641 ctacggttac atcaaccaca ccctgtctct ggacggtgac ccggttgacg tactggtccc
    26701 gactccgtac ccgctgcagc cgggttctgt gatccgttgc cgtccggttg gcgttctgaa
    26761 aatgaccgac gaagccggtg aagatgcgaa actgattgct gttccgcaca caaagctgag
    26821 caaagaatac gatcacatta aagacgttaa cgatctgcca gagctgctga aagcgcagat
    26881 cgctcacttc ttcgagcact acaaagacct cgaaaaaggc aagtgggtga aagttgaagg
    26941 ttgggaaaac gcagaagccg ctaaagctga aatcgttgct tccttcgagc gcgcaaagaa
    27001 taaataagtt cttctagcgc aataaccctg aacgccgggc ttcggttagt aagggttttt
    27061 ttatgcccgc gataaataaa ctctctattc caccatcatt attctcagcg gttgcaaggc
    27121 ttgaacggta agaacaagca aacccgacca ccattttgct gttcatagcc acttgctgga
    27181 agttagccga cctcactcat actcaccgtt caaagcgtgc ccccgtttta cccctgcaca
    27241 tttgtgaacg ccagcagagt tttttatgcg attgcagata tggaaagcgg ctcggagaga
    27301 aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga tacatcgcat aaaacaacgc
    27361 cacccgaagg tggcgtgctt ttattattac ttatccctgt ctaaccagtc gccgctttca
    27421 attagcttta atccatcgac gggtcgttga taaacgtaca tccatgcact cccgtacggc
    27481 gtctgaatca actggcgcgc gtattcaccg cccctggtgc gcaaggcatc aagttcggcc
    27541 agcgtggcgt tgtcaatacg ataaacttca ccgtgtactg ttccgttccc cggaactgcg
    27601 cctggatagt ggcccaggct atacaactgg tagttatcga tactgaaatc gcccagtaac
    27661 tgggcattgg tcatccagtg actgttgcct tgtttgtggc gtaaactgcc gtagacaaat
    27721 attcgcattg ctaaaactcg aactgataga gcaaatccag tgcctggtct acaccagaca
    27781 cggcttccag atatagctta ggcatcaggc gataacgtaa cgtgagtgtt gctatagagt
    27841 caaatatacc cacgccgtat ttcacttgca gacctggcaa tacatagccg ctgaccacta
    27901 cctgggagga gtcgcctact ccctgggtgt cgagcgctaa attgcttacg ccaaacgtct
    27961 cgccgatttt acccacaatc tggccacttt gcgcaacccc cagaccaatc agcatcgagg
    28021 tcattgccgc actgtcgctc tgatcgctct ccagcccttg tccacgtagc aaataagaca
    28081 atgcagcttg ttgcgacatc gccgggtcag agaagatctc cgctttcggt tcgtccgcca
    28141 gaccagtgac gcgaactccg gcgattacgt cgtcttctgt agcatccggg ttacgaatag
    28201 cttcaatatt aagatacggt tgatccggcg gaccagagaa cagtaactca cctttacgca
    28261 caatcagatc ctgaccatag gcatggaagc gcccttcagg gatgttgatc tgcccgttca
    28321 ggcccagccc ttgtttgtcc tgaacgacgt taagatcgcc cgtcagccgc gctttcaggc
    28381 caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat caggttactg ttaatcggaa
    28441 tcgacgccgt tttcggctct tccggttgca ggttatcgtt aagcatcacc acatcgctgg
    28501 agacgcctac tgcgctttcc ggcagatcgt gcaccacgat gcgcgcccac gggacatcca
    28561 cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa gacaacatct ggcgatacat
    28621 ccattcgtac catcggcggc acggtgatcc gcactttact gcctttcgcc gttacccgcg
    28681 cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt caggtagatt tcaccctgct
    28741 gggtccgtac tgtaccggca agcgtcgagc gcataccatt aaagttgacc gcaagctggc
    28801 tcggttgcat atcaaacggc ataaagttgc cgtcgatatc cacacccgta acctgaagct
    28861 gaccaaacaa ctgcgggctt tgcacatcac cacccagacg caagttggca ctcaccatcc
    28921 ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat cgcaaggttg aagttacgga
    28981 tattgacgtt gccaccaaga ttacggcggc cttgcggatc ggtcacctgc acctgtccat
    29041 caaactggcc gttattggtc aggcggatgg tccagcccaa ttcggcacgg ttgttacgca
    29101 attccgccgt cagattcagt gtctgaaacg ccacgggcag cgccgcatcg ttgacggttt
    29161 gcgttacctg cacgttacgc cccgaaaggg tgatactgcc ctgcggcagc ccctctttcg
    29221 tggtgtccca ggcaacatcc gctttacccg tgaagatacc gctggcctga gtggtttctg
    29281 gcataaacgg tttcagcatg gcgaggtcga agcggttgag attcaccacc gcacgccctt
    29341 cggcccccgc atcgatagtt tgcggcacgc acagttccgc attcgggtta agccagcagt
    29401 gtggcccgat gctgattttt tgctccttat tgcggtaatc cagcgcaata tcgcgggtca
    29461 gcgaccacgg gccgaccggc gtctggaagc gggtattgct aagagttccc ttccagcgtt
    29521 cttctttgcg atcaaaactt cctgccagat taagctgccc ggagacaggc tcgccctgaa
    29581 tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc attcagggtg acgaggttga
    29641 tattgacatc cggctgcgaa atttgctcaa cgcgtacgtc gagtttaccc gcaatctgat
    29701 cggtggattt gatatcgcct tccacgcgaa cctgcgccac ggaaagttcc tgccagcgca
    29761 ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc caccgtgccg cgtactttca
    29821 ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt atccagaccc ggcgcgttga
    29881 tggtggcatc aagattgaga tcttttaccc ccagctcgcc tttcacttcg gcactgtttg
    29941 gccccagttc cagatgcagc cctgggatca tccactgcat ataactgttg cctttcagcg
    30001 tgccgtcaac gttcactttg ttctgtttga cgttaccggt cagcttcagt tctggcacct
    30061 ccatctgcca ggtgccgccg tacaggctac cgcgggtttt aatcaagcca ttgagtttcg
    30121 acggccactc tgggatctct ttggcggtgt taatgccgtt aagcgttagc tcaccgcgcc
    30181 aactaatggc ctgctgccag tcgagcaacg ccttgagttc agttttccct tccagcgccg
    30241 cgacggtgag tttgtcgaga ttgacctgct gttcattacc tttggcatca agggtaatgg
    30301 tggctggcgg gatctccagt cccttcactg ccgtacgcat agagagcgtg taatcggtca
    30361 ttttgccggt aagtttcagt ttcagatcat ccgcctgata ctgcttctca ccagtgaacg
    30421 gccagtaaat ctgtttgctg ttcacttcca cgttgagcgg caatccggct tccgccagtc
    30481 gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag attaacgcca atctccagct
    30541 gttcgcgcag cgcgccgccc acttttagct tcactttttc acctttcaac ggctccacgt
    30601 tcagtgtgct gttgagggtg atatccaccg gccagttgtc tgacagctgc gccgtgccgc
    30661 tggcgttgac gatcccctgg ttggaatcga tatccagggc gtccagttta gtattgccgt
    30721 caatgctgct cactttcagc agcatggtgc gcacggtgat gtccgtgtcg cccgtcacgc
    30781 gtaactgctc gcctttaaac tcttcaatgt tcaggttaag cggcaaatgc acgtcggtca
    30841 tttccggcaa taccgggcga gaaaagagat ctttcagcgt ttcgccgagc ggcttttcat
    30901 ccggctgcgg attttcaatc ttcggttcga cgacctcttc ctgcgccact tccgccactt
    30961 tcggcagagc aatcagcagg cctttcagcg acgtcggttt cagggtcagg gttttctcct
    31021 gccagttcag gccggaggtg aagtccatca ccgataccgt ggtgtcatca atcttgatgt
    31081 tgacgttatc cagcgccacc cgtgtcaggg tgatgggata cggcgtggag agatccagcg
    31141 gaccgctatc ttcctcttct tcaacctgtt cagaaggagg cattttttta ctgtcgatgt
    31201 tgacctgaat gtctttcagc gccaggtcat taatacaaac gctactgttc cacaggcact
    31261 caagcccgac cgccagatgc agattgcccg cttttaccgc cacgcctggc tgctcataac
    31321 gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac cttgccaata tccagtcctg
    31381 gtacccagcg atccgccgct ttaaatacca gatgcaggcc actggtggtg cccaccagaa
    31441 acgccaccga tcccagcaac agtaagataa cgataaccac gccgaggcta atttttttcc
    31501 ataaactcat aattctggcc ccagaccgat gtaaaactgt aacccgtgtt cgtctttatc
    31561 cgcgaccggt acggcaaaat cgagtttgat tggcccgacc ggcgattccc agcgcacgcc
    31621 gaccccggta ccggttttaa agtcgctgcg gcgaatatcg cttaccgctt cgccactatc
    31681 gacaaacacc gcgccccacc attttccggt cacgttgtac tggtactcca gcgatccggt
    31741 tatcaacttc gaggcccctt tcaggtcacc gttggcgtat ttcggagcga tagatttgta
    31801 tttgtagcca cgaatactgc ggtcgccccc ggcgaagaaa cgcagatccg gcggtacttt
    31861 gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt gtaacaaaac gatggcgatc
    31921 gtacagtgtg cggatccaga cgttttgtgc ctggaaaacg gagaaatcga catctgagcc
    31981 ccaggccgtg ttggagtagt cgatagagta gcgttgcgag tcgccccagg ttggcatcag
    32041 gccaccacga gaacgcgtgc ggctaatcat caccccagga taaaacagca tcgtggtgtt
    32101 ggtaatttca ccctgagtaa agtggtcgag actccagcgc aggttaatgg cacgctgcca
    32161 gccgctggag agatcccagt agcgagaagc caccagcgta gtggagtcag attcggtatc
    32221 gttcaggtca gtgcgcttaa aaccgccctg caccaaataa tattgttcca gtggattctt
    32281 cagcagtggc attttatagc tgaagtcgag ggtctgttcc ggcgcggaaa tactggtact
    32341 ggtggtcaaa ctgtgaccgt atgagttcat ccacggcttt ttccacgtcg ctttcacgcg
    32401 cggtcccacg tccgtagagt aaccgacccc ggtttcgatg gtgttttctg ttcgcggcga
    32461 aaccacgccc gtcaatggta atactttcgt ttcgcgcgct ttatcaaatt gtggagccac
    32521 caccaccgag ttaaaccagc cggtagcaga aagtcggcgg ttcagttccg ccagatcttt
    32581 cgattcgtac tcatcgccct ctttaaacgg caccagattt tgcaggtatt catcgcggat
    32641 ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt tcgccactgt tataatcaat
    32701 atcccagaag gctttatgca ggccgagcgc aatgcccagc tgcgctttgg taaattcgct
    32761 atcgaaataa cctttacgca acgcaatgct ggttaaggac tttttgaaat tttcataatc
    32821 gccctggttc agcaccgtgc caatagccgg gcgagtatcg agcaatttca aatagtcttt
    32881 atcggtccgc gcgccgccgc gcaataccac atcggtgccg ccaattaaca ccggcacgcc
    32941 tggcgtgact ttggcgatca atacctgccg ccctttcttt ggcggtggac ggagatcaaa
    33001 ttcaatggtc ggctggtaat aacccagcgc tttcagacct tcgcggatgg catcatcgac
    33061 gcgtgcgcga aagcgacggt ctggcgccac ttcatcactt tcaatcgtag aaagctgcgc
    33121 acgaacgttc ttttccagct gtcccgatac cccctcgacc tgtagacgga cgttcgcggc
    33181 gacggcagat ccgcttaagc agagtaagct tacacagcat aactgtcgga tatagcgcac
    33241 attttctcct gaatatcctt ttttcctgcc cctggaacgc cgttaaacgg cttaacaaaa
    33301 atccagtaat atggattaaa aagcagacta aaccccaaat atttcttatg ttttacttta
    33361 gacctattca cggtggttat tgtgtgcaaa tacgcctctt gttacaacct taaccccaat
    33421 gaccgatttt cgggagagcg acaccatgag tttatttgat aaaaagcatc tggtttcccc
    33481 cgccgatgcc ctgcctggac gtaacacccc gatgcccgta gccacgctgc atgcggtcaa
    33541 cggtcactca atgaccaatg tacctgacgg aatggagatt gccatttttg cgatgggttg
    33601 tttctggggt gtggagcgtc tgttctggca gttacccggc gtttacagca ccgccgcagg
    33661 ctataccggc ggctatacgc caaatccgac ttatcgggaa gtgtgctccg gtgatacggg
    33721 tcatgccgaa gcggtacgca ttgtttacga tccttccgtc atcagctatg aacagttgct
    33781 acaagtattt tgggagaatc acgatcccgc ccagggaatg cgtcagggca atgaccacgg
    33841 cacgcagtat cgttcagcga tttatccact gaccccagaa caggatgctg ctgctcgcgc
    33901 cagtctggaa cgttttcagg cggcgatgct tgccgccgat gacgatcgtc acatcaccac
    33961 ggaaatcgcg aacgccacac cgttttatta tgccgaagat gaccaccagc aatatctgca
    34021 taaaaacccg tatggttact gtggaattgg cggaattggc gtctgtctgc cgccggaagc
    34081 atagcgttac gggtacaaat gtagattgtt gataaagtgc tatttgtcta tgccggatgc
    34141 ggcgtaaacg ccttgccagg cctacaaaat cgtccaaaat caatatattg tggggactgc
    34201 gtaggcctga tcggcatagc gcatcaggca gtttttcatt tatcagcagt ctgaatttta
    34261 accctctggc ggctttacag taccttacgc tatactagcc actgaaaatg ccggttcact
    34321 ttcttcgaat cggctttcaa tgtgtatttc acacaaatta atcaacttcc ccttccgagg
    34381 atctggcctg aaaggtcgga taagatatgt taaacagtat tttagtcata ctctgcttga
    34441 tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct tgccgcttca cgcaaaatca
    34501 aacttaaact gctggctgat gaaggcaata taaatgccca acgcgttctg aatatgcagg
    34561 aaaatcccgg catgttcttt accgtggtcc aaatcggtct gaacgcagtg gcgattctcg
    34621 gcggtatcgt cggtgatgcg gcattttctc cagcttttca cagcctgttc tcccgctata
    34681 tgtcggcaga actctctgag caactgagct ttattctctc tttctcgtta gtgactggca
    34741 tgtttatcct gtttgcggat ttaaccccga aacgcatcgg tatgattgcg ccagaagctg
    34801 tggctttgcg tatcatcaac ccgatgcgct tctgcctgta cgtttgcacc ccgctggtgt
    34861 ggttcttcaa cggactggcg aacatgatct tccgtatttt caaactgcca atggtacgta
    34921 aagatgacat cacttctgat gacatctacg cggtagtgga agctggtgcg ctggcgggcg
    34981 tgttacgtaa acaggaacac gagctgattg aaaacgtctt tgagctggaa tcccgtaccg
    35041 ttccgtcgtc aatgacaccg cgtgaaaacg tgatttggtt tgatctccac gaagatgagc
    35101 aaagtctgaa gaataaggtg gcggaacatc cgcactctaa gttcctcgtc tgtaatgaag
    35161 atattgacca catcatcggc tatgtcgatt ctaaagacct gctgaaccgc gtgctggcta
    35221 accaaagcct ggcactgaac agcggcgtac aaatccgcaa cacgctgatt gtgccggata
    35281 cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc aggtgaagac ttcgcggtga
    35341 tcatgaacga gtacgcgctg gtagtgggga tcatcaccct caacgacgtg atgaccacgc
    35401 tgatgggcga tctggtcggt caggggctgg aagagcagat tgtcgcccgt gatgagaact
    35461 catggctgat tgacggcggc actccgattg acgacgtcat gcgcgtgctg gatattgacg
    35521 agttcccgca gtcgggcaac tacgaaacca tcggcggctt tatgatgttt atgctgcgta
    35581 agatcccgaa acgcaccgat tcggtgaaat tcgccggcta caaatttgaa gtggtggata
    35641 tcgataacta ccgcatcgac cagctgctgg tgacccgaat cgacagcaag gccaccgccc
    35701 tttcgccaaa actgcctgac gctaaagata aagaagaaag cgtcgcgtaa cccccagaaa
    35761 catcaacggc tcctgaatca ggagccgtct tattactgca tagcactttg gttaagccat
    35821 ctctgtttgc agacgcataa cctgacggtt aacttcggac atcactgaca aatgcagctt
    35881 atccttcact tttgggataa gaatcttacc cttatcaaat tcaaaagcgc caacgtcctt
    35941 gatgtataac cgtccacgga acaggatctt tacgtacttc gccacctgaa gtgggttgta
    36001 ccgttggaaa attttcattg ttatctcctg ctgagtatta cgcctgtgcg gtgccacaat
    36061 cggcccaact attatgaggc gcaaatttta atgcttagtg actatagact atccgggcaa
    36121 tgtttccacc gcgtataact ttttttacct aaaggttaca attattcaga attatctttt
    36181 taccgaagcg tggtcttcag tataagcatt catttttcat atgatttgtg cgcttgaccg
    36241 caaactggca tcacacttgc gggaaattcg ataaatagca catatgatta aaactcagac
    36301 ccaagtggtc ggaccacctg catatcataa gaaggaaaca ccatgaccct acgcaagatt
    36361 ctggcactca cctgcctgct gttgccgatg atggcttccg cacatcagtt cgaaaccggt
    36421 cagcgagtgc cgccgattgg cattaccgat cggggcgagt tggtgcttga taaagatcag
    36481 tttagctaca aaacctggaa cagcgcgcag ttagtgggaa aagtgcgagt actgcaacat
    36541 attgctggtc gcacctctgc aaaagagaaa aacgcgacgc tgattgaagc gattaaatca
    36601 gcaaagttac cgcacgatcg ttaccagacc accaccattg ttaacaccga cgacgcaatt
    36661 ccgggttccg gcatgtttgt gcgcagcagt ctggagagta ataaaaagct ttatccctgg
    36721 tcgcagttta ttgtcgatag caatggcgtc gcgcgcggtg cctggcagct ggatgaagaa
    36781 agttccgctg tagtggtgct ggataaagac ggtcgcgtgc aatgggccaa agacggggcg
    36841 ctcacaccgg aagaggtaca gcaagtgatg ggcctgctgc aaaagttgct caaataatgg
    36901 caataacata aattctgcaa aaataatttt cccctatcgt tatctatggg ataggggaat
    36961 taagcctctg agttcttacg ccgaactcat actggtaacg ctagttgctt ttttttaaac
    37021 gcctcaatgg tcgatttata tttctcgcaa atcttttctt cggtaatatc actatctgcg
    37081 gccagattcg cacttactat cgcatccact tgttcggcta attcgacttg ttggcaacct
    37141 gcattaacca gaaaattata aataagagag taatcttcat cctccaaaac aacagaggaa
    37201 ccattacgaa aacgttcatc ccctccatag attttaagcg caaagcccaa agattctatg
    37261 actatggcaa aattatcatc aattattttt tcgtaaatat accatccatc gcattcttta
    37321 attccacctc ctggtatttg gaggtcaaat ttaccccgtt ttaatttact tactatatca
    37381 tccaatacag cataacctaa agacacgtca ccaaacgcct ggcgaatata acctttaatc
    37441 ctttccggta tgtcagactg ttttttctgc agaatatccc aggcatacaa tcttctgttt
    37501 tgttctaacg gaaactcgcg gtttttgaaa tccatgaatg agttatactc atttattgtt
    37561 atgggaattt cttcgtattc aaatccactg cctcgcaatg tcattatata ttcggcaccg
    37621 ttatctttaa ggctgaaact gataagaaac ttatccatgg cgttaacagt ttcatttttt
    37681 gatgaaagta cctgataact atttttatca atctgagttg cgcaacgaaa gaactccctc
    37741 aaagttaaca ggctatcata atttgaaatc caatcttttt tattaaatgc atgctggata
    37801 ttgttataaa aattgtttga atatgttttt gtatgcgatg attttacgaa taactccacc
    37861 gcaccaataa aattattgtg tctgatcctg ggtaacatat ggctacctta ttttatttgg
    37921 ttattttatg tcgccatta
//
LOCUS       DACBVI010000026        37770 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.026, whole genome shotgun sequence.
ACCESSION   DACBVI010000026 DACBVI010000000
VERSION     DACBVI010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.026"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            79..2322
                     /gene="pdeF"
                     /locus_tag="IU325_06730"
     CDS             79..2322
                     /gene="pdeF"
                     /locus_tag="IU325_06730"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HAP5808736.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDVKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2361..3902)
                     /gene="ppx"
                     /locus_tag="IU325_06735"
     CDS             complement(2361..3902)
                     /gene="ppx"
                     /locus_tag="IU325_06735"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HAP5808737.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3907..5973)
                     /gene="ppk1"
                     /locus_tag="IU325_06740"
     CDS             complement(3907..5973)
                     /gene="ppk1"
                     /locus_tag="IU325_06740"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HAP5808738.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6144..6782)
                     /gene="purN"
                     /locus_tag="IU325_06745"
     CDS             complement(6144..6782)
                     /gene="purN"
                     /locus_tag="IU325_06745"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP5808739.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAIVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6782..7819)
                     /gene="purM"
                     /locus_tag="IU325_06750"
     CDS             complement(6782..7819)
                     /gene="purM"
                     /locus_tag="IU325_06750"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP5808740.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8144..8770
                     /locus_tag="IU325_06755"
     CDS             8144..8770
                     /locus_tag="IU325_06755"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP5808741.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8856..10145
                     /gene="uraA"
                     /locus_tag="IU325_06760"
     CDS             8856..10145
                     /gene="uraA"
                     /locus_tag="IU325_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP5808742.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10195..10941
                     /gene="hda"
                     /locus_tag="IU325_06765"
     CDS             10195..10941
                     /gene="hda"
                     /locus_tag="IU325_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HAP5808743.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(11047..11406)
                     /gene="arsC"
                     /locus_tag="IU325_06770"
     CDS             complement(11047..11406)
                     /gene="arsC"
                     /locus_tag="IU325_06770"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HAP5808744.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11427..12890)
                     /locus_tag="IU325_06775"
     CDS             complement(11427..12890)
                     /locus_tag="IU325_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HAP5808745.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANDAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13103..14164
                     /locus_tag="IU325_06780"
     CDS             13103..14164
                     /locus_tag="IU325_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP5808746.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14202..15050)
                     /locus_tag="IU325_06785"
     CDS             complement(14202..15050)
                     /locus_tag="IU325_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HAP5808747.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNVMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPPPFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQEP"
     gene            complement(15072..17084)
                     /locus_tag="IU325_06790"
     CDS             complement(15072..17084)
                     /locus_tag="IU325_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP5808748.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLAHIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRCMPGESVLLTQTLQTRQPTLTHRADDLFLWQRDPLLHLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSVSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17114..17527)
                     /gene="hyfJ"
                     /locus_tag="IU325_06795"
     CDS             complement(17114..17527)
                     /gene="hyfJ"
                     /locus_tag="IU325_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HAP5808749.1"
                     /translation="MAEECREIVFWMLRKKFIASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSAWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(17520..18278)
                     /gene="hyfI"
                     /locus_tag="IU325_06800"
     CDS             complement(17520..18278)
                     /gene="hyfI"
                     /locus_tag="IU325_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HAP5808750.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLREADDPRLNSIVQQLFRVLRGLHG"
     gene            complement(18275..18820)
                     /gene="hyfH"
                     /locus_tag="IU325_06805"
     CDS             complement(18275..18820)
                     /gene="hyfH"
                     /locus_tag="IU325_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HAP5808751.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDYQQNTRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
                     KQRATLINDDTDEPLVAKEQL"
     gene            complement(18830..20545)
                     /gene="hyfG"
                     /locus_tag="IU325_06810"
     CDS             complement(18830..20545)
                     /gene="hyfG"
                     /locus_tag="IU325_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HAP5808752.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWSVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKIVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(20535..22115)
                     /gene="hyfF"
                     /locus_tag="IU325_06815"
     CDS             complement(20535..22115)
                     /gene="hyfF"
                     /locus_tag="IU325_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HAP5808753.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGHSATRTVTVLHGLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLLIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGELGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22120..22770)
                     /gene="hyfE"
                     /locus_tag="IU325_06820"
     CDS             complement(22120..22770)
                     /gene="hyfE"
                     /locus_tag="IU325_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HAP5808754.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFVAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMTLLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(22782..24221)
                     /locus_tag="IU325_06825"
     CDS             complement(22782..24221)
                     /locus_tag="IU325_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
                     /protein_id="HAP5808755.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGGVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(24238..25185)
                     /gene="hyfC"
                     /locus_tag="IU325_06830"
     CDS             complement(24238..25185)
                     /gene="hyfC"
                     /locus_tag="IU325_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HAP5808756.1"
                     /translation="MRQTLCDGYLVIFALAQAVTLLVLTPLFTGISRQIRARMHSRRG
                     PGFWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFHFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     DSTHIEMISKTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKLGLGLKQVVMASLFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25196..27214)
                     /gene="hyfB"
                     /locus_tag="IU325_06835"
     CDS             complement(25196..27214)
                     /gene="hyfB"
                     /locus_tag="IU325_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HAP5808757.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFDFADLTLRMDSLSAFMVMVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNLFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAIDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVFIALSASWLAPKIMHIAHAFTN
                     TPPVTVTSGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWEHAMAPSGNGVMQPLRVVFCALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(27214..27831)
                     /gene="hyfA"
                     /locus_tag="IU325_06840"
     CDS             complement(27214..27831)
                     /gene="hyfA"
                     /locus_tag="IU325_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HAP5808758.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQADGSLKDGEENAPTQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(28084..28554)
                     /locus_tag="IU325_06845"
     CDS             complement(28084..28554)
                     /locus_tag="IU325_06845"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HAP5808759.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(28554..29126)
                     /locus_tag="IU325_06850"
     CDS             complement(28554..29126)
                     /locus_tag="IU325_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HAP5808760.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29272..30150
                     /gene="dapA"
                     /locus_tag="IU325_06855"
     CDS             29272..30150
                     /gene="dapA"
                     /locus_tag="IU325_06855"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP5808761.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30167..31201
                     /gene="bamC"
                     /locus_tag="IU325_06860"
     CDS             30167..31201
                     /gene="bamC"
                     /locus_tag="IU325_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HAP5808762.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31414..32127
                     /gene="purC"
                     /locus_tag="IU325_06865"
     CDS             31414..32127
                     /gene="purC"
                     /locus_tag="IU325_06865"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP5808763.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32295..33158
                     /locus_tag="IU325_06870"
     CDS             32295..33158
                     /locus_tag="IU325_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808764.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33173..35188
                     /locus_tag="IU325_06875"
     CDS             33173..35188
                     /locus_tag="IU325_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP5808765.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPVAENHCFPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTANNDAILWRQNQQFSL
                     AHFAPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVIAAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIVGALQYTHDLDYLSVSFGYTEELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFTHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKLLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            35262..35960
                     /locus_tag="IU325_06880"
     CDS             35262..35960
                     /locus_tag="IU325_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP5808766.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGNDDVIEMM"
     gene            complement(36041..36241)
                     /locus_tag="IU325_06885"
     CDS             complement(36041..36241)
                     /locus_tag="IU325_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808767.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(36269..37396)
                     /gene="dapE"
                     /locus_tag="IU325_06890"
     CDS             complement(36269..37396)
                     /gene="dapE"
                     /locus_tag="IU325_06890"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HAP5808768.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHAGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVEWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(37400..37756)
                     /locus_tag="IU325_06895"
     CDS             complement(37400..37756)
                     /locus_tag="IU325_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HAP5808769.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKIIDAASAAALMTEMPAIIKRPLLCTPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9142 a         9814 c         9974 g         8840 t
ORIGIN      
        1 taatcatcat tcgaagtcag gtgggatgcc tgtctgaata cacctccttc aggatgtggg
       61 ggattctgct gagcatctat gaaactgaat gcaacttata taaaaatacg tgataaatgg
      121 tgggggcttc cgctgttcct gccttcttta atcttgccca ttttcgccca cattaatact
      181 ttcgcgcata tttcttccgg tgaggttttt ctcttttatc tgcctctggc tctgatgatc
      241 agcatgatga tgtttttcag ctgggcggca ttgccaggga tcgccttagg gatttttgtc
      301 cgcaaatatg cagagctggg tttttacgaa acgctctcat taacggctaa ttttattatc
      361 attatcattc tctgttgggg cggttacagg gtctttactc cccggcgtaa caacgtttca
      421 catggtgata cccgtttaat ttcccagcgt atattctggc agattgtgtt tcctgcaacg
      481 ctgtttctga tacttttcca gtttgctgcg tttgtaggat tactggcgag cagagaaaat
      541 ctggtcggcg tcatgccttt taacctcggg accttaatca attatcaggc cttactggtg
      601 ggcaatctga ttggtgtccc gctgtgctac ttcatcattc gtgtagtgcg aaatccgttt
      661 tatttacgta gttattattc gcaattaaaa cagcaggttg atgtcaaagt caccaaaaaa
      721 gagttcgcac tctggctact ggcattaggt gcgttgttgt tgctgttatg catgccgtta
      781 aatgaaaaaa gcacaatttt tagcaccaat tataccttgt cattattgct gcccctgatg
      841 atgtggggag ccatgcgcta tggttataag ctgatttcgc tgctctgggc ggtcgtgttg
      901 atgatcagca tccacagcta tcaaaattac attcccattt atcctggcta taccacgcag
      961 ctgaccataa cctcctccag ttatctggta ttctctttta ttgtcaatta tatggctgta
     1021 ctggcaaccc gtcagagagc ggtagtcaga cgcattcagc ggcttgcgta tgtggacccg
     1081 gtggttcatc tgccaaatgt tcgcgccctg aatcgcgcgt tgcgtgatgc cccctggtct
     1141 gcgctttgtt atttacgcat ccctggcatg gaaatgctgg ttaaaaacta tggcatcatg
     1201 ctacggattc aatacaagca aaaactttct cactggctgt caccattgct ggaaccgggt
     1261 gaagatgttt atcagctatc gggtaacgat ctcgcgctgc gactgaatac agaatcgcac
     1321 caggagcgca ttaccgcact ggatagccat ctcaagcaat ttcgtttctt ttgggatgga
     1381 atgccgatgc aaccgcagat tggcgtcagt tactgctatg tgcgctcgcc agtgaatcat
     1441 atctacttac tgctgggaga gctaaatacg gtcgccgaac tttccatcgt gaccaacgcc
     1501 ccagaaaata tgcagcgtcg cggggcaatg tatttgcaac gcgaattgaa agataaagtc
     1561 gcgatgatga atcggctaca gcaggcgctg gaacacaacc attttttcct gatggcccag
     1621 ccgattaccg gtatgcgtgg cgatgtctac catgaaattc ttctgcgtat gaaaggtgag
     1681 aatgatgaac tgatcagccc cgacagcttc ttgccggtcg cgcacgaatt tggtttatcg
     1741 tcgagtatcg acatgtgggt cattgagcat acgctgcaat ttatggctga aaacagagcg
     1801 aagatgcccg ctcaccgttt tgcgattaat ctgtcgccaa cctcggtatg tcaggctcgt
     1861 tttcccgttg aagtcagtca gctgctggct aaatatcaaa ttgaagcgtg gcaacttatt
     1921 tttgaagtca ccgaaagtaa tgctctgaca aatgttaagc aggcgcaaat caccttgcag
     1981 catcttcagg aattaggctg ccagattgcg attgatgatt tcggcaccgg ctatgccagc
     2041 tatgcgcggc ttaaaaatgt gaatgccgat ttgcttaaaa ttgacggcag ttttatccgc
     2101 aatattgtgt caaatagtct ggattatcag atagtggcgt cgatttgcca cctggcgcga
     2161 atgaagaaaa tgcgggtagt ggcagagtac gttgaaaacg aagagatccg cgaggcggtg
     2221 ctctctttgg ggatcgatta tatgcagggt tatcttattg gtaagccgca accgttaatt
     2281 gatacgctga atgaaatcga acccattcgc gaaagtgcct gaataatgcg ggccgacatt
     2341 tctcgtcggc ccgcaaagca ttaagcggcg atttctgggg tactttcttc ttcaattttc
     2401 aaccgccagc cagccacgcc ttcccagtat tcttgctcct tttccagatc aagcagtacc
     2461 agcgcattct gactaaacca gtcatgcggg aaacgcagtg tccagtgact gtcatcggta
     2521 atcagcgtca atgttggcgg tgtagtggtt gcctgacgtt gattattgag taatacgcca
     2581 aggcgcaata gctgaatcag tggcaggaac tgtttcttct taaacaaggt aaagcgcggc
     2641 agatcgtcga gcttaatcgc tttacggtga tagcgcacca gcgtcgccat catcaactgc
     2701 tgttcctgat taaaacccgg caagtcgctg ttttgcagaa tataagctga gtggcgatgc
     2761 aaaccgctgt ggttgatatt caacccgacc tcatgcagca tggcggccca tcgcagtagc
     2821 gcctccagtt gcggatgcgc cagcttcggt tgctgttccc gccactgttc gtacatttgc
     2881 atagtggtat ccagtacccg tcgggcctgt tcgctgtcga tgtgatactg gttggcgagg
     2941 ctgctggcgg tgcgactacg cacatcctga tgacggaaac gtccttccat ttcatacagt
     3001 acgccttcgc gtaacgcccc gtcagaaagg cgcagttcac ggatggctaa agcatcaaac
     3061 acaccgcata aaatcgccag tcccggaacg aagactgttt tccgctcttc ggaaagaccc
     3121 ggtaaactca gcgatgcgaa attacggtga cgtaaaactt cttttaccag tttttccaga
     3181 cgttccgggg taattatccc gtctttctcg cccatttcca ttaacacttc atgggcggct
     3241 tttatggtac cggaagcgcc cattgccacg ttccagccct gaatacggaa ttgccaggtt
     3301 aaagtttcca gtttttgtgc tgccgccatg cgagcgcgct gaaaattctc tttattgatg
     3361 accccgccag ggaagtaaag ctgggcaaag ctgacacaac ccatccggcg gctttcaacg
     3421 agaatcggtt cgaaattttc accaatcacc agttccgtag atccgccgcc aatatcaata
     3481 accagtttgc gacctttctc cggttgggta tgttccacgc ccataaaaat cagacgggct
     3541 tcttcattac cggaaataat ttcaatcggg tagggaatga ccttttccgc gcgtttcaga
     3601 aagtcagtgg cgttcagcgc ctgacgcagt gtatgggtgc caactataca gacgctggca
     3661 ggagaaaacc cttgcagccg ttcggcaaac agcgacagac agttcaaacc gcgcgtcatt
     3721 gcctcttcac tcaacatatt atctggcccc aggccgtccg ccagatgcac ccgctgtttc
     3781 aggcggccaa taatctgcat ggcaccatct accacacggg ctatgaccat gtgaaaactg
     3841 tttgaaccaa gatcgaccgc agcaaactcc tgcggacgag gggatttatc gtgtattggc
     3901 atagggttat tcaggttgtt cgagtgattt gatgtagtcg taaatcgcca actgcgcccg
     3961 tactttgcgg cgattgccgc ggggaacgta gcgattactg agttctttat cgatataacg
     4021 tgctttgact gtatcgctga acaatatgtc gatgatgtcc agcacccgct gcttcaggcg
     4081 cggatcgagc agtggcgtcg ccacttcaat acgataatca atattgcgcg tcatccagtc
     4141 ggcggaagaa aggtagacct ttttatcgcc gccattttca aaaatataaa cccggtcatg
     4201 ttcaaggtaa cggtcaacaa tactgatggc acgaatgttg tcgctaatgc cttccagatt
     4261 ggggatcagc gaacacatgc cgcgaaccag cagattaacc ggtacgccgg agctggaggc
     4321 cgcatacaga cgatcaacca ggcctttatc gacaaggtta tttagcttca gggtgatacc
     4381 actgggcagc ccttgctgcg cgttggcgat ctcgcggtcc accatttcat acaacaggcg
     4441 gcgggagttt tgcggcgaca ccattaaata atcaaatgtc accggacggt atgggttttc
     4501 aataaagttg aacacccgcc gtacttcgtt ggtgatgcgc gcatcggcgg tcagcaacga
     4561 atagtcagta taaagacgcg cggttttttc gttaaagttc ccggtcccga tgtgtgcgta
     4621 acgcaccact tcaccgtttt ctttacgtga aatcaggaac agtttggcgt gaattttcag
     4681 ccccggcgca gagaagataa cgtgcacgcc tgcttcggtc aggcgcttcg cccagtgaat
     4741 gttggcttct tcgtcgaaac gcgcctgtaa ctcaaccacc acggtgactt tcttaccgtt
     4801 gtgtgcggcg tggatcatcg agtcgatgat gcgtgaatct ttcgccacgc ggtaaatgtt
     4861 aattttaatc gccagtacgc tcgggtcgaa cgaagcctga cgcagcagtt ccagcacatg
     4921 ctcaaaggta tgataaggat aatagagcaa cacatcgcgt tcgcgaatgg catcaaaacc
     4981 attgcggaac tgggctttat caaaccaaat gtggcgtaaa cgcggcagtg gtttgttcac
     5041 cagattggct ttgccgacat tggggaaatt aataaagtct ttaaaattat gataacgacc
     5101 gccggggacg atggagtcgt agcgggaaat agtcagtttt tcgcgtaaca cttcaaccag
     5161 cgcattgggc atatcgcgct gataaacaaa acgcaccggc tcagcagtta aacgctgctt
     5221 gagactggaa gacatcaact ccatcaggct ggcttccatc tcatgcacta aatcgtattc
     5281 ggcatcgcgg gtcatcttca ttgaataggc attcagtgcg tcataatcaa agaagccttt
     5341 gaaaatatca tcaaggcagt aacgcagaat gttatccaga agaatcatcg gcttgcgtcg
     5401 acgcggcgct tctggcggta aattcacaaa gcgcggcact ttatctgatg ggatctccag
     5461 cagcgcgtaa cggatggtat cgccacggat aatttccacc gccagatagg tgtaatcatc
     5521 tttcaggaac tgcactaagt cagtgtcagg attgattaaa atcggcgtaa tgtgctgacg
     5581 cagatactgc ttaaaataat gacgcagcca gttttgttga ttgacggaga gctggcgttc
     5641 attaatcagg aagatctggt tgcgcgccat ctccagcagt aactcgttgt agaggccgtc
     5701 gaattcctga tcggctttca gcacccggga ctgaattttg cccagtaaat ggcgggaatg
     5761 agagttggag ccttgttctt cgctaataat gatgcgtcgc ttcagttcag cgaagcggac
     5821 tttatagaac tcatcaaggt tattggaata gatccccagg aaacgcatcc tttcaatcag
     5881 cgggttagat ttgtccgccg cttcctgaag cacgcgttca ttgaacgata accaactgag
     5941 ctctttttcg atgtatagct tttcctgacc cattaccact tttactccgt tttattcacg
     6001 ggacactgcc tggatattat ggcgagcaat attggcagat gtccaactat gccagaaaag
     6061 ggggaaaaaa actgctggag cttgcgattt acaggtgaat tgaaaaacgc cagcggcaga
     6121 gctggcgctt taactacggg gcattactcg tcggcagcgt agccctgcgg cggcagacgt
     6181 tgaccatcca gccacgcggc gttttcgtgc attttcagac gaccatcggc aaaccagcta
     6241 atcaccagtg gataaatagc gtgttcctgg gtttgcacgc gggcggtgat gtcatcctcc
     6301 gtatcaccag caaataccgg gactttcgcc tgtaaaataa ccgggccacc gtccagttca
     6361 tcggtgacga aatgcaccga tgtaccgtgc tcttcatcgc cattttccag cgcctgacga
     6421 tgggtgtgta atccgggata tttcggcagc agagaagggt gaatgttcag caaacgcccg
     6481 gcatagtggg agacaatcgc cgggctgaga atgcgcataa aaccagccag tacgaccaca
     6541 tcgggtgcgt acatgtcgat ttcatgaatc aactcccggt cataggcttc acgactgtca
     6601 aacgcgctgg cgatgagcgt atgcgttgca atacccgcct ggcgggcgcg ttcaaggccg
     6661 aacgcgtcgg ccttattgct gaaaactgcc cgtacggtgc ctttaatttt attggttttg
     6721 caggcgtcaa taattgcctg caaattactt ccgttgccgg aaataagcac cacaatattc
     6781 attattcgat aaccacgcgt tgttcggaat cagaggcttt gatgataccg attttccacg
     6841 cgttttcacc gttggcattg agcagggcga gggctttgtc cacttccgga gcaggcaggg
     6901 caataatcat cccgacgccg cagttgaagg tgcgatacat ttcatggcgc tcaacgttac
     6961 ctgccgtttg cagccagttg aacacttccg gccactgcca ggatgattca tcaatcactg
     7021 cctgagtatt atctggcaat acgcgcggaa tgttttccca gaagccgccg ccggtcaggt
     7081 gcgcaatagc atgcacatcg accttttcaa tcaactccag cacggacttc acgtaaatgc
     7141 gggtcggtgc cagcagatga tcggctaatg gcttaccatc aagttcggtg gtttgcggat
     7201 cacaaccgct gacttcaaga attttgcgca ccagcgaata gccgttcgag tgcggaccgc
     7261 tggagccgag tgcaatcagc acatcgccat cgctgacttt agagccgtcg atgatttctg
     7321 atttttctac cacgccaacg cagaaaccag cgacatcgta atcttcaccg tgatacatcc
     7381 ccggcatttc tgccgtttcg ccacccacca gtgaacagcc cgattgcaga caaccttccg
     7441 cgatgccgct gatcaccgct gaagcggtat caacatccag ttttccggtt gcgtaatagt
     7501 cgaggaaaaa cagcggttct gcaccttgca ccaccaggtc attaacgcac atggcgacca
     7561 gatcaatacc aatggtgtcg tgacgtttta agtccatcgc cagacgcagc ttggtaccta
     7621 cgccgtcagt gccggaaacc agcacgggtt cacgatattt ttgcggcaat gcacacagcg
     7681 caccgaagcc gcccagaccg cccatcactt ccggacgacg cgttttcttc actacgcctt
     7741 tgattcttcc aaccagagca ttacccgcgt caatatcaac accggcatct ttgtagctaa
     7801 gagaggtttt atcggtcact gcttgggtcc ccacgcgtta cttgcggtag aaaaataaaa
     7861 ttcggcgcaa ttctaacagg gaaagcaaac gtttgcgaga ctgctttaca caaccttttt
     7921 gcacgtcttt tccccaggcg cgcggcgaaa gaatacttgt accagggcaa aggatcgttt
     7981 tcggatggaa taatcttctt ctataaccat ttgaatataa aataacttta tctcaaaccg
     8041 ttatcatttt gactaaagtc aacgaaaata atattgccgc cttgaagaaa ggaggtataa
     8101 tccgtcgatt ttttttgtgg ctgcccctca aaggagaaag agtatgaaga tcgtggaagt
     8161 caaacaccca ctcgtcaaac acaagctggg actgatgcgt gagcaagata tcagcaccaa
     8221 gcgctttcgc gaactcgctt ccgaagtggg tagcctgctg acttacgaag cgaccgccga
     8281 cctcgaaacg gaaaaagtaa ctatcgaagg ctggaacggc ccggtagaaa tcgaccagat
     8341 caaaggtaag aaaattaccg ttgtgccaat tctgcgtgcg ggtctgggga tgatggacgg
     8401 tgtgctggaa aacgttccga gcgcgcgcat cagcgtcgtc ggtatgtacc gtaatgaaga
     8461 aacgctggag ccggtaccgt acttccagaa actggtttct aacatcgatg agcgtatggc
     8521 gctgatcgtt gacccaatgc tggcaaccgg tggttccgtt atcgcgacca tcgacctgct
     8581 gaaaaaagcg ggctgcagca gcatcaaagt tctggtgctg gtagctgcgc cagaaggtat
     8641 cgcagcgctg gaaaaagcgc acccggacgt cgaactgtat actgcatcaa ttgaccaggg
     8701 actgaacgag cacggataca ttattccggg cctcggcgat gccggtgaca aaatctttgg
     8761 tacgaaataa agaataaaaa taattaaagc cgactttaag agtcggcttt tttttgagta
     8821 aagcgcctat aacacataat acagaggata atactatgac gcgccgtgct atcggggtga
     8881 gtgaaagacc gccactttta cagacaatcc cgcttagttt gcaacatttg ttcgccatgt
     8941 ttggtgcaac cgtcctggtg cccgtcttat ttcatattaa cccggcgact gtactgttat
     9001 ttaacggtat aggaacactg ctgtatctct tcatctgtaa agggaaaatt ccggcttatc
     9061 ttggttctag ctttgccttt atttcaccgg tattgttact gttgccgttg gggtatgaag
     9121 tcgcgctggg cggctttatt atgtgcggcg tgctgttctg cctggtttct tttatcgtga
     9181 agaaagcggg gaccggctgg ctggacgtgc tgtttccacc tgcggcaatg ggcgcaatcg
     9241 ttgccgtcat cggtctggag ctggcgggcg tagctgccgg tatggcaggt ttactcccgg
     9301 ctgaagggca aactccagac tccaaaacca tcatcatctc cattaccacc ctggcggtta
     9361 cggttttagg ttccgtgctg tttcgtggtt tcctggcaat tatcccgatt ttaattggcg
     9421 tgctggtggg ctacgcgctc tctttcgcaa tgggaattgt cgataccacg ccgattatta
     9481 atgctcactg gtttgcgctg ccaactctct atacgccgcg cttcgagtgg tttgccattc
     9541 tgactattct gccagcggcg ttagtggtta ttgccgaaca cgtagggcac ctggtggtaa
     9601 cggctaatat cgtcaaaaaa gatctgctgc gcgatccagg tctgcaccgt tcgatgtttg
     9661 ctaacggctt gtcgaccgtg atctccggct tctttggctc cacgccaaat accacctacg
     9721 gggaaaacat tggcgtgatg gcgatcaccc gtgtttacag tacctgggtt atcggcgggg
     9781 cggcgatttt cgctatcctg ctttcctgcg tcggtaaact ggctgctgct atccagatga
     9841 tcccattgcc ggtgatgggc ggcgtttcgc tgctgcttta tggtgtcatc ggtgcttccg
     9901 gtattcgtgt tttgatcgaa tcgaaagtgg attacaacaa agcacagaac ctgatcctga
     9961 cttccgtgat tttgatcatc ggtgtcagtg gcgcgaaggt aaacatcggc gcggcggagc
    10021 tgaaaggtat ggcgctggcg accatcgtcg gtatcggctt aagtttgatc ttcaaactga
    10081 ttagtgtgct gcgccctgaa gaagtggtgc tggacgcaga agacgcggat ataacagaca
    10141 aataatgtac cgaccgggca gtgttgctgc ccggttcaaa catcatggga ttctgtggta
    10201 aacttctcgc gattttgtga aatcctggtt gaggtatctc tgaacacacc ggcacagctc
    10261 tctttgccac tttatcttcc tgacgacgaa acctttgcaa gtttctggcc gggggataac
    10321 tcctctttac tggccgcgct gcaaaacgtg ctgcgtcagg aacatagcgg ttacatctat
    10381 ctctgggcac gcgaaggcgc ggggcgcagc catctgctgc acgcggcttg cgcggaattg
    10441 tcgcagcgtg gcgatgcggt gggctatgtc ccgctggata aacgcacctg gtttgttccg
    10501 gaagtgctcg acggtatgga gcatttgtcg ctggtctgta tcgacaacat tgagtgtatt
    10561 gcaggcgatg agttgtggga gatggcgatt ttcgatctct acaatcgaat tctggaatcg
    10621 ggcaaaacac ggttgttaat caccggcgat cgtccaccgc ggcagttgaa tctgggatta
    10681 ccggatctcg cgtcgcgtct cgactggggg cagatctaca aattgcagcc actttctgat
    10741 gaagataagt tgcaggcgct acagttacgc gcgcgtttgc gtggttttga actgccggaa
    10801 gatgtggggc gtttcttgtt gaagcggctc gacagagaga tgcgcacgtt atttatgacg
    10861 ttggatcagt tggatcgtgc gtcgattacc gcgcaacgta agctgaccat tccgtttgtg
    10921 aaagaaattc tgaaactgta gacaagacgt ttattgtcgg atgcgcaccc atagtattcg
    10981 tcggccggat aagacgcggc aagcgtcgca tccggcaatg tgcttcaact agcgatggag
    11041 cacttcttaa cccacgattt ccagcacctg ctccggtgga cgaccaattc gcgctttgcc
    11101 attcgcgacc acaatcgggc gctccattag cttcggatta tccaccattg cctgaatcag
    11161 cgcctcttcg ctaagcgaac tgtcagccag gttcagttct ttataaagat cctctttctg
    11221 gcgcatcaat tcacgagcgc tattcatccc cagcattttc agcaaatcac gcagcgttgc
    11281 cgcatcggcg ggtgtctcaa ggtagagcac cacttctggc tccacgccgt tttctttcag
    11341 caggttcagc gtttcccggc tctttgaaca gcgtgggtta tggtagattt ttacctgttt
    11401 ggtcattgtt cttcctttaa tgcgaattac atcttggtat aaggcttaaa gcgttcctgc
    11461 agctggcgca actggtcgat gcgcgcatcg taacgtgctt gttgcaggct gcctaatttc
    11521 acctgcgaac tggcgctact caacaaggaa atagcctgat cgagtcgccc ggcgagcgca
    11581 taaccttctg ctcgcgcagc cagttcctga tcgcggttat ttagcgcggc ttccgcctgt
    11641 gccagcaaat cccagccgtt gctgtcatct ttattattaa aggtgtagcg attcagaata
    11701 ttggccgctt cttgtggttg accgccttgc agataagcgt tcgccaggtt gagctgcaac
    11761 accggattgg tgcgtaaatc acgggcattt ttcagacgat tgatagcgtc attggctttg
    11821 ttttgcccaa gatcgatatc agtagccaga tcgagatacc atgcgttgcc aggttctgcc
    11881 gccagtaacg gttgcagcgt tttacgcgcc tcgtcgtatt tattggcttc catcgcctgt
    11941 aaagcacgac catattgtgc tgctcgttgc tgacgaacgt ttcctttcgc ccattcatcc
    12001 agcaaatcac tggtgagctg gttacgtccg gaattataca tccccagtgt gcgcgctttc
    12061 gccagataga aatcttccga cgactgcacc accatcggac gcatctgatt agcacggttg
    12121 cgggcatctg ccagacgact ttccggcaac gggtgagtca ataaaatttc cggcggacgc
    12181 gaggagtaac gcgcctgatc gagcaatttt tcgaggaagg tcggcatcgc ctgcggatcg
    12241 aatcccgagc gttgcagcac ctgaatacca atgcggtccg cttcctgttc attttgctgg
    12301 gtgaaactga tcatcccctg gcgcgttccc gccagtgtac cggtcagcgc agccatcccc
    12361 gcctgcggac tggccatcgc cagtaaaata gaacctaatg cgccgaccca ggtcagcggc
    12421 gcgctgcgct gctgatcttc catcgctcgc gccaggtgac gttgggtgac gtgggagatt
    12481 tcgtgcgcca taactgaagc cagttgactt tcgttatcgg aataacggaa cagggcagag
    12541 tgcagcacca cgttgccgcc aaagaaagca aaggcgttaa tttcgtcgtt gttgatcaga
    12601 aagaaatgaa acggtgtctt aaccgaattg gcatgcgaaa ccagacgcat acccagcgaa
    12661 ttaatatatt gcgttaacag cgggtcatta attaacggcg cgctgccgcg tagctggcgg
    12721 acataatagt cgcccatctg catttcctgg ccaatggaaa gcgtgcttcc tgcggaggtt
    12781 cccatatccg gcaaggtgtc tgcgctgtcg gcaaacgccg gggctacctg accaatggtc
    12841 atagcagcaa tgagggttgc aaccaggttt tttttcaact gcctgaacat aacctctatc
    12901 ctgtatttct gaagatagtc atttgaccga tactgtgaaa tatcgttccc accgcgccgc
    12961 ctttgagtgt aacggcgaag attagcccac tcaaatgact tttcagtttc ctgaatccgt
    13021 cataaaaagc gaagtctttt ttgtgacatt tcgatacaat tgcggatcgc aatcccgctc
    13081 ttgaggttca gggaaggtct ttatgctcga aatgttgatg caatggtatc gccgccgttt
    13141 tagcgacccg gaagcgattg ccttgctggt tattttagtt gccggatttg gcattatctt
    13201 tttctttagt ggcctgcttg ctccgttgct ggtggctatt gtgctggcct atttgctgga
    13261 atggccaacc gtgcgcctgc aatctattgg ctgctcccgc cgctgggcga cgtcgattgt
    13321 attggtggtt ttcgtcggta tattgctact gatggcgttc gtggtattgc ctatcgcctg
    13381 gcagcagggc atctacttaa ttcgcgatat gccggggatg ctcaataagc tttctgactt
    13441 tgccgccacg ttgccgcgcc gctatccggc gttgatggat gcgggcatta ttgatgcaat
    13501 ggccgaaaat atgcgcagtc ggatgctgac catgggcgat tcggtggtga aaatttccct
    13561 cgcctcgctg gtcggtttgc tgaccattgc cgtctatctg gtgctggtac cattgatggt
    13621 cttcttcctg ctgaaagaca aagagcagat gctgaacgcc gttcgccggg tgctgccacg
    13681 taaccgtgga ctggcaggac aggtgtggaa ggagatgaat caacaaatca ccaactatat
    13741 ccgcggcaaa gtgctggaga tgatcgtggt ggggatcgcc acctggctgg gattcttact
    13801 cttcgggctg aactattcgc tgctgctggc ggtgctggtc ggcttctcgg tacttattcc
    13861 gtacattggc gcatttgtgg tgaccattcc ggtggttggc gtggcgctat tccagtttgg
    13921 tgctggcacg gaattctgga gctgcttcgc ggtgtatctg atcattcagg cgctggacgg
    13981 caacctgtta gtacctgtgt tgttctccga agcggttaac ctgcatccgc tggtcattat
    14041 tttatcggtg gtgatcttcg gtggtttgtg gggattctgg ggcgtattct tcgccattcc
    14101 gttggcgacg ctgatcaaag ccgtgattca tgcctggccc gatgggcaaa tcgcgcaaga
    14161 ataatcttca gcccgacgaa ctgtttcgcc gggctgattt tttagggttc ctgacgtaaa
    14221 taaatagccc gataacaaat actcaccagc actgcaccgc cgataatatt acccagcatc
    14281 accgggagca gattggcggt aataaaatgg ctgaccgtca gtgccggaaa attgtctgcg
    14341 ctactgtgcg ccagttgcca gaagggggga ggggcgaaat ggcgaatggc aatggcgaac
    14401 ggaatcacaa acaaattggc gatacagtgc tcaaagccac ttgcgacaaa cagggtgatg
    14461 ggcaaaatca tggcgacgat tttgtcgcat aatgaacgtc cgcaataact catccacagc
    14521 gccaggcaaa ccatcacatt gcacataatg ccgaggctga ctgattcagt aaatgtatga
    14581 tgcattttgc cctcggcgca gtgtaaaacc gcgactcccc acatcgcgtt ttcactcatc
    14641 accagcccgg aaaaccagat taacaaactg aaacaggcaa tacctgccag attaccgcag
    14701 gccaccagaa gtgcattaat cagccatgtt cgccagctaa taacgccccg actttttgcc
    14761 atcaccgtca ttaccgacga ggtgaacagg ctggtgccgc aaaccgctag caaaataaag
    14821 ccgagtgtaa agcaaaggcc gcccaccaga tgggttaatg cctgtgaaga cggggcatcg
    14881 gcgataacgg aaaggtaaaa agtaaatcca atcgccataa acacgccagc ggacatcgcc
    14941 attaaaaaga ccgatacttt gctgcgggca attttatggg cagcaatccg ttcagcgatt
    15001 gccgcttttc tggcgctcaa ctgcaagtcg aaagagagtt tgtttctcat gcatactcct
    15061 gcaaaaacag attacaacac ctcgcgaacc gagatcccca gccgctgcat tcgtgacagc
    15121 agcgtggtgc gcttcatccc taatcgcgtc gccgcgccac ggggcccggc aacaatgcca
    15181 ttggtttctc gcaatacctg aacaatgcgc tgacgttctt cttcgtcatt ctctggcgtc
    15241 gtcgggtgca gcaactgtgc cattgaactg cgaagcgctg aatcttcatt tagcgtcggc
    15301 agtaaacggc tttgtcggac attaagatgt aaattcagac tgttaccgcg agtcagtagt
    15361 accgctcgct caatcacgtt ttccagctcg cgcacgttgc ccggccaatc ccacgacatc
    15421 aactggcgta gtgcctcggt cgggatggcg tcaattgcgc gattcatatg gcgcgccatt
    15481 ttttgcgtga aatgttttgc taaaagaggg atatcttccg gacggtcgcg cagcggcggc
    15541 aattccagtg ggaagacatt caggcgataa aagagatcgc tgcgaaactg gcgatcttca
    15601 accatttgcc acaaatcacg gttggtggcg gcaatgactc tgacattcac cgggatcgtt
    15661 ctactcccgc cgagacgctc aatctcccgt tcctgcaata cgcgcagcag tttaggctga
    15721 agttctaacg gcagatcgcc aatttcatcg agaaacaacg tgccgccatc ggcaatttca
    15781 aaacgaccac gatgggtatt aatcgcacca gtaaatgccc ctttgtcatg accgaataac
    15841 tcactttcca gaagactggc ggggatggca gcgcagttga ttttgaccag tggcttgtcg
    15901 cgtcgtgggc taagttgatg gatcgctctg gcgatcacct ctttgccggt tccggtttca
    15961 ccacaaatca acaccgtact gacgctcttc gccacaatat ctacctgctg gagcaaatct
    16021 tccattgcct ggctttgata gataatgtca ccgacgccca gattcgaaag gagctgctcg
    16081 ctaagctggt ggttttcttg ctgcaagctt tcctgcaaat cggtcatgct gcgccaggca
    16141 tcggcattgc caacggcaat agcgatgcga tcggcaatat gttgtagtag ctggcagttt
    16201 tcctcactaa agagagtgga agaggtatgc gccagcaaca atgcgcccgg tgtatggttg
    16261 ccaaaggtga gcggtatcag gagcgcagat tcgcagccgt tagatgcaag taagtgtaat
    16321 aacgggtcgc gttgccagag aaacagatcg tctgcacggt gcgtcaaggt cggttgtcgg
    16381 gtttgtagcg tttgtgtcag caatacactt tcgccaggca tacagcgtgg cagacacgca
    16441 caatgtgagg cagaaagatc gctgcaccac aggctgaact tctcgttctt tcgatgatcg
    16501 cccagtacca tactgactga agccagaccg aaaaaatgat ggatctcacg agcgacgtca
    16561 gcgatcagat cgtcgagatc aagatgtgac aacactgcat tagtgatatc caccagtacc
    16621 cgatgatgat cgcgatcttt ctccagcaaa cgcagcgcca cctgctgttc ttcatcattg
    16681 agccatgatt gcagaaccgg agtgattatg tgtgccagcc agttaaacag tgtgcaatcc
    16741 tggggagtaa aacggctgcc gtgagtgcga ataaagcgta gctccgccat tccactactg
    16801 ccgagaagga cggcgtgacg cgaataatcg accatctcac cctgatactc tgttgttgcc
    16861 gtcacatcgc tgccatcatc atggtagcgg taatagaagt tgcttttgag aacatgcagt
    16921 tcaataagtt gtaccgggct gaacggcggc ggcagcggga tgaaggcgcg taataaagac
    16981 gccttgccgt gtttctgcgc taaccgctcc gcgagcattc cgtttaccgc ttcaattgtt
    17041 attccttgcg gcggggcaaa catcgcctcg tctgacatag ccatcaacag tccttattaa
    17101 tgagaaatcc ttctcacgca atttttctgg ccatcagata gatggccggt tcctgcacaa
    17161 tttcatccaa cagatgaatg aattgaatgc tacgcgcctg ccacgccgac gtcgcgtcat
    17221 ctgccagtgg cgcgaatgcc cgggtcaaca gggcggtgtg gctggcgtca ataacaatct
    17281 caccaaaagt catcaccccc agcatcttac gtcgggcttg ctcctcttcg accagtgcaa
    17341 gccaatcttc gtattccgtc agtgggcagc ggaaggcgac attcagacaa tcaatcacgc
    17401 caacgtgatg gccgatagcc aacgagtaat acattacctg ggagctgtgt tccggcatct
    17461 cgtcgctact ggcgataaat ttttttcgca gcatccagaa aacaatttcc ctgcactcct
    17521 cagccatgta acccccggag aacgcgaaag agttgctgaa cgatactatt gagacgcgga
    17581 tcgtcggctt cgcgcaacca ggtgttaacc cgatttcctg tgggatcgtc gcttaaatgg
    17641 cgcaggagtc gatcgcaaat ttctcgccct tgacgatagc ccgccagccg cctcgcttct
    17701 cgctcaatgg cgatacgctg tgatggcggg atctgcggcc acagcggttg catggtcacc
    17761 ccggtgggat cgcgataatc caccgcgtga atcttctgtt gcagcaaacc gagcgccacg
    17821 gcgaaaccgt gaatggtggc ggccggtgtt ggcgggcagc cggggatcca aacatcaatg
    17881 gggacaatgg tgtcgctgcc gccccagacg ctgtagagat cgtggaaaat accgccgccg
    17941 acaccacacg cgccgtagga aacacagatt ttatgatcgg gggcagactc atatgcccga
    18001 agtgcaggca tacgcatcgc ccgggtgact gcgccagtaa ataacaaaat atcggcgtga
    18061 cgcggtgatg aaacaacctt aatgccaaaa cgttctgcgt cgaatacggg tgtgatggcg
    18121 gcaaaaattt cgatttcgca ggcgttacag ccgccgcaat cgacgcgata aacgtaggcc
    18181 gagcgacgga tatcctgtag caaatgctgc ttcatctttt gcgtttgctc gtccagcgtt
    18241 atgggctggc tgacatgttg cgtaagcact ggactcatag ctgctcctta gccaccagcg
    18301 gttcatctgt gtcgtcgttg atcagcgtcg cgcgttgttt gcattccggg cagacgctcg
    18361 cttgcgcccg caacatttcg cggttttgtg gtgcattttg ttgctgtgcc aacagttctg
    18421 cagccagtgc gacggttttt tgcggggcaa acgggcgttc gcaacggctg caacgttgta
    18481 gatggaacgt cgcgcgggta tagagatcgg ctttattggt gacggtcagt tcaaagttat
    18541 tggtaagctg aatggctctg gtcgggcaca cttcttcaca acgtccgcag taaatacaac
    18601 gtcccagata gagctgccag gtgcgcgtat tttgctggta gtcggtctgg atagtcagcg
    18661 catttgccgg acaagcacag gcgcaggcac cgcaggcaat acattggctg ggcatcaggt
    18721 ccggtttccc gcgaaagcct gggctgacct ccagtggtgc gaagggatat ttcaccgtcg
    18781 cggttccggc gcgcataata gttttcagta acttcagcat cttctgtcct tacttcagcg
    18841 gcgaacggtt acgatcaatg ccgtagcgtt cgatctcttt atacggcacg atttttgact
    18901 ggcgcttgcg cacatcaacc agcgtcacac ggtcggtaca ggagtagcag ggatcaaggc
    18961 taccgataat tagcggtgcg tcagaaacgg tattgccgcg cagcatgtaa cgcagcactg
    19021 gccagttggc gtaggtggcg gcacgacagc gccagcggaa caatttttgg ttatcaccga
    19081 gcatgctcca gtgcacgtct tcaccacgtg gcgcttcaac aaagcccagc gcgaatgcgt
    19141 gaggtttata gctaaagcct tcggtcagca gtggggtatc tggcatgttg tcgagggcaa
    19201 attccagcat tgccagcgaa tcaaacgtct ctttgacacg gaccatcacg cgggagaaga
    19261 catcgccgcc ggtaaaggta aacagcgttt ttggaatatt gccgtagtcg gcgtaggggt
    19321 gatcaaagcg caaatcacgg gcaaaaccac tgccgcggat cagcggcccg acagggctat
    19381 aatcacgggc gatttgtcgg tcgagaatgc caatgccctg agtgcgttgt tccatattcg
    19441 gcgtggcaag cagcatctct accagctccg acacgtcggc gcgcatctcg cgcactagtt
    19501 tcagcgtttg cagacgttgc tctttgagaa tatcgcggcg aacaccacca atcagattca
    19561 gaccgtaggt tttacgcgac ccggtcagca attccgccat cgtcatcgac ttttcccgca
    19621 cgcggaaaaa ttgcataaag ccggtatcaa aaccaacaaa atgacaggag aggccaaggt
    19681 tgagcaaatg actgtgcagc cgttcgactt ccagcagaat cgagcgaata gtatgcgctc
    19741 gttgcggcac ctcaatcccc agtgcatttt caaccgagtt ggtataggcc acactgtggg
    19801 caaaaccaca aatcccacac acgcggtcgg ataagaaggt cacttcgttg tagcccatcc
    19861 gcgtttctgc cagtttctcc atgccgcgat ggacataaaa caggcggtaa tcagcatcga
    19921 caatttgctc gccatccaca aacaagcgga agtgacctgg ttcatcggag gtgatatgca
    19981 gcgggccgac agggatcacc cgcgcatcgc tgttgccctc gttgataaac ggatacgttt
    20041 cggtatcagt cgtcggttct gggcgcagtc gataatccat cgcgtctttg cgcagcggat
    20101 gcatatcttc cggccagtca tcgggcaaca ccagccgacg ctgatccggc aggccaaccg
    20161 gaatcagccc gtacatatcg cgaatttctc gctcgcccca gaccgcggca gggacgcgcg
    20221 gtgtgactga cggaaactcc cgactgtcgg catcgaccag tgccttcacc acgctccagc
    20281 atttttcggc cccttccata gaaagggcgt aataaaccgc gtaatgaccg ttaagtgtcc
    20341 gctcgtcgtt gccaaacagg accggaagcc agccatcatg ttgataataa agataatgta
    20401 caacgtcagg cagcagattg attttcaccg taatggtgac ctgttcaggc gtttgtcgct
    20461 cttcatccag caccgcgccg gggaactgcg ttttcagggc ggcgagaatc gcttcgccac
    20521 gatttgacga tgaattaacg ttcactgtgt ttctccgaaa cagatgcggt gcctgaaggc
    20581 aaaaaatcat gccaggtgct acgttgtgca ggcaggtcgt gcgtccctga gaggactata
    20641 gtggaagcgc ccgccaggat cctgatgaca ggttgtggaa tatgcgttcc catcgccagc
    20701 atcatgacca gcagaatcac cattggcgag gtggtcaacc agccaagttc accccggtta
    20761 acggcctgcg gcggtttcgc cattaatacc cgtgcagcca tccgtaccag gcccgccagc
    20821 accagcgtta acagcaataa cagcaggacg ataagcagca gatgattacg cgccagtccg
    20881 gcggtaacgg tcataaattc gctaagaaaa atgttgaagg gcggcatccc ggccagcgcc
    20941 agcgcaccgc cgccaaacaa cacggcggta aatggcatga ttttgagcat cccacagacg
    21001 acgttgagat cgcgcgtgcc gtacttgagc agtacattgc cggaaccgca gaacaacagc
    21061 gttttggcca gactgtggtt taaggtgtgc agcagcgcgg caaaaattcc cagcggcccg
    21121 ccaatgccta acgccaccgc gaccagtccc atgttctcca cgctggagta cgccagcaga
    21181 cgcttaatgt cccgctgtac cagaatgaaa aatgccgcca cggcaaccga caacatgccg
    21241 aagatgagca acaaccggtt ggggaaatcg ctgccgatgg cctggcaaat aatgatgtaa
    21301 tagcgaataa gcaccaacag cgcgcagttc agcaatacgg cggagagcag ggcgctgacc
    21361 ggactcggcg cttcactgtg agcatctggc agccaggcgt gcatggggaa tagcccggtt
    21421 ttggtgccaa agccaattag cacaaacaca aaggccaaca gcattaatgt tgggtcaagc
    21481 aaggacgatt gcttaagaac ctcgctccag aatatcgcca tttctgcctg cggcataacg
    21541 ctggcggcgt tggcgtatac cagcacggta ccgaacagac caaaagcgac accaacagta
    21601 caaataatga tgtacttcca tgcagcttcc agcgatgaac gctgaccgta aatgcctacc
    21661 agaaacgccg agcttaaggt ggtggcttca attgccgccc acatcacaat caggttattg
    21721 ctggtaacaa ccagcagcat ggtgaacaaa aacagatgga agaaaccgta gtaatcgcac
    21781 agcgtaacgg gtgaaagctc gccgtgtgcc acttcatgac gcatgtagcc aatcgagtaa
    21841 acaccggtga gaaagccaat cacaccaaga atggcgagga acaaaccgcc cagaccatca
    21901 atgtgaagcc acagtcccgc agcgaatatt tctcccgcat cagcggcagt ttggaccacc
    21961 cacagtgcca gaatcagcag cagtgtgatc cctaagccat gtagtactgt caccgtgcga
    22021 gtcgcagagt gcccccgttt ccggcaggca aaacagagca gcgaaaaaag cagcggcgtg
    22081 agcaggagta aagcgaacat cacagaataa ctcatctcat tatcccttca gcgcggtcaa
    22141 gttattcacg tccagcgtgc cgtgggtacg ccatattttt cttgccagta acaccatcac
    22201 aatgacggcg aagatggcgt cggtagcgat acctatttcc accagttccg gtgctcgcca
    22261 ggcaagaagc gccagcacca gatgggagcc gttttccatc aggcagtaac caaaaatttg
    22321 ccgcaggata ttgcgctggc tgacaatgca cagcaggcca agcagaaaat gacctaacgc
    22381 caccgccagc gccggtttca gcccggtagc catcggtagc ttcacgggct gaacgacaaa
    22441 tgcgcaaagc aggacaatta acgccgcgag cagtgtcatc attgccggac cgaataacgc
    22501 tttttccggg atgttctggg gaatatttcg tgcagcgtaa gtcatgatta acggtaccag
    22561 cagcactttg gtgataaagg cgctggcgga ccagatcagc agttgctctg cgacgaacag
    22621 gcacgagagg gtggcgaaaa tagacaccag caccagcgac tggcaggcgt aaaatccgca
    22681 tgacaggcga tagcttttga cgctaatcac aaacagcgat gtcagcatca tcagtcccgc
    22741 cagattattt acgatcatag aaccggtcat tcatcatctc cttactgcaa ccaggtcgcg
    22801 gcgattacgc tggaaatcag cgacatcaca atcagtacaa tcaacaccag gcgcattgat
    22861 cctggcagcg gtgcggcatc ggcgacggcc tcgctcggtt tgccaggcac gacgcgacca
    22921 aaccagcgaa taaaccaggc gaaactggcg accgattcaa tcatcagcag aatcatggcg
    22981 ggcagcagga tccagtactc cactgacaac gcaaaaccgg cggcaaacag cgggaattta
    23041 ctgaagaagc cgttgaacgg cggtacgccg gtaatcgcca gcgctgccac gcagaaaccc
    23101 acgcctggca acggcagggt gtgcaatacg ccacgcagac gcggcaacaa gcgcgtgccg
    23161 cagctgtaac tcagcgcacc tgctacaagg aaaaataggc ttttagcgaa cgcgtggttg
    23221 acgatgtagg cgatgctgcc ctccagcgcc agccgcgagc cgaagatgga gagcgacaag
    23281 ccgaaaaaca tccagccaag ttgagtgatg gtcgaccagg ccagcaaccg cttcatatcc
    23341 tgctgtggca aatacatcag gaagccataa agaatggtga ccagtgccat aaccatgcca
    23401 acgccgccaa tcacatgcgg gatattgccg ccgtcgataa tggcgcgggc aaaaatgtaa
    23461 acgcccactt tcaccatcga tgcggcgtgg agataggcgc tgatcggtgt tggcgcttcc
    23521 attgcatccg gtagccacgc ttgcatcggt agctgggccg atttccccca cgcggcaaac
    23581 aggatgccac cataaaccag atagcgtgcg tcgccgtgta actcgctcat cgcgctgagc
    23641 gcgaacgttc cggtctgcaa aaacagcgtg gcggcggcaa gatacaaccc caacgaaccg
    23701 atatgagtga taagcaacgc ttttagtgct gaacgctgcg ctttatcgct ctggtaataa
    23761 ctgatcaacg cccaggagca gccgcccgta atttcaaaaa acaacaactg accgagcagc
    23821 gtcgaggaga gtaccagtcc cgccatcgcg ccgataaaaa ccagcaaaaa tgcgtaataa
    23881 cgattcgtgc cgttatgcgg gtgttcgcga tttttatccg tcagataacc cgtggagtag
    23941 atcgtgacca gcaaaccgag aaacaccacc acaaacagaa tcagcgtact cacgcgatca
    24001 atgaccaggc caaacaacgc cacacccccc acgttgacca acgtcaacgt gacggcaact
    24061 ttatcggcct gataaaacgc ggagatcagt gacaacatgc acagcgtggt cagcgcggcg
    24121 aacaaaaccc cccattcggc ggcccgacgt tgtggcgaaa acgaaacgac cagtgcgccg
    24181 ataaaaggca gcaataacgt cgtcagagca agattttcca tattccgtca gtgctcctta
    24241 cagaccggtt aaccagaaga cccatgcaag cgcagcaagg ctgaagccaa gccaggtcac
    24301 atggtgaatg agtaaaaatc gcccgcgtgc cagcgtgttt tctgcgattg aggccagtac
    24361 aaaaatcagc aaaaccttga gcagcgtaac gacaagtgaa gtcagcaggc aggcgagaga
    24421 aagctcttgc gcgcgcccaa agggcagaaa cagggccaca aacagtgatg ccatcacgac
    24481 ctgtttcagc cccagcccca acttcgccag cgccagcccg gcaccggaat attcggtcag
    24541 cgggccttcc tgtaattcct gttctgcttc agcaacatca aagggaattt ttcccatctc
    24601 aatgaagcag gcaaaaccac aggccagtaa cgccagtacg gtggttagcg gcgagttcca
    24661 gcccgtcgcc agcgtcttgc tgatcatctc gatatgcgtg gaatccgcta tcagcgccaa
    24721 taccagcagt gagagaatga gcatgggttc gaccagaatg ccgagcgtca actcgcgact
    24781 ggcaccgact cccgcaaacg ggcttccggt atccagcccg gaaagagcaa agaaaaaatg
    24841 aaacagggca agaagataga taagggtgat cagatcgccg ccgcccgcaa aaggggaaac
    24901 agtaataaat agtggcaggg ccatcgccag caccagcatg ctgctgatta atacccacgg
    24961 catcaggcgg aacatcagac ctgaagatgt cggcgcaact tcctggcgtt taaacagttt
    25021 gtggatatcg cgataatcct gccagaaccc cggtccgcga cgggagtgca ttcgcgcgcg
    25081 tatctgccgg gaaatccccg taaaaagcgg ggttagcacc agcagagtca cggcctgtgc
    25141 taacgcaaaa atgaccagat atccgtcgca aagagtttgt ctcatggtga tttccttaga
    25201 cggcaatagc gattagcagc acaaccagtg cggcgaccac atacaggcaa tagagacgaa
    25261 agtcgccact ttgcagatgc tgtatttctt tggccagccg ttgggtggcg ctcacgatgg
    25321 ggcggatcac ccgctcatcc cagaagggtt ctgtgctctg agccctggcg gtgacgtgcg
    25381 caagaccttt gttcagcctc agcgtagggt cgagctgttg tcgtagacga aatagcgcac
    25441 aaaagaccac acgcagcggc tgcatcacgc cattgcctga cggggccatc gcatgttccc
    25501 agccgtagcc gcatgcccag gcatctccag tgcgacgaaa cgctgcgcgg cgcgaacgac
    25561 accacagcca gtaaaggcca ggcagcaaag gcatcgccag tagtaacagc aacagtaatg
    25621 agggagtgac ccgtgtatga aacgtgccgg gtacaagtgc tattccgctg gtgacagtga
    25681 cgggaggggt attggtaaac gcatgggcaa tgtgcattat cttcggtgcc agccaactgg
    25741 cactaagcgc aatgaatacg cagagtgccg cgagcagtag catggcgacg atcatcgtat
    25801 ttggcacttc ctgtgcctct tcagcgtgtg tactgcgcgg cgcgccacag aaagtaatac
    25861 cgtacatttt gacgaagcac attactgcca gcccaccagt gactgccagc atcaccatag
    25921 caataggacc cgcaagttgt agcgctacgg cttccacacg acttagtgag aacagcgact
    25981 gccaggtgta ccattcgctg ataaaaccat tcagcggagg aatggctgat atcgcgaggc
    26041 aaccaatcag gcatgctgcg gctgtccacg gcatccgttt cgccagtgcc cccatttttt
    26101 ccatgtcgtg ggtatgcaaa cgcgaaataa tcgcacccgc gccgagaaac agcagccctt
    26161 tgaacagcgc atggttgagc agatgaaaca gcgcgccgag cagtccaaca acggtgagca
    26221 gcgggtcgtg cagtgacaga ccgaccatcg ccacaccgac cgccagcaaa ataatgccga
    26281 cgttttcgac ggtgctccag gccagcagcc gtttgatatc ctgttccgcc agcgcataca
    26341 gcacgcccag gagcgcggag attgcgccga tcgccatcac cagaatgccc caccacagcg
    26401 gcaaacccgt ttgcgccagc agatcaatcg ctactttcag gatgccgaaa ataccgattt
    26461 tgaccattac gccagacatc aacgccgaag cgtgcgatgg tgcggcaggg tgagcgcgtg
    26521 gcaaccagct gtgcaacggc atcatcccgg ctttcgcgcc aaaaccgaaa aaggccagca
    26581 ggaacaccgc cgacgccagc cccggagaaa gtgaaagcgt gcggaaactg gcaaaatcga
    26641 ggctgccgct ttcgcgcccc atcagcaaga aggcgatcat aatcagcacc gatccggcgt
    26701 gggcgataaa aaagtagagc atgccagcgt tgatcgacgt tttatcctgc ctggcaatga
    26761 ccagaaacca ggaagacagc gacatcattt caaacagcac gatgaaccaa aaagcgttgt
    26821 ccatcaccag cagggcaacc atcgatgcga tgaaaagatt cataaagaag cccatcgccg
    26881 ccgcgccttt accctcgtat tcgcgcatat aagtcaatga atagagcgaa caaaccacca
    26941 ccagcaagga gataaccatc accataaatg ccgagaggct atccattcgc agtgttaaat
    27001 cggcaaagtc aaaaggcgtg gcaaaacggg cgactaaagt tacgcctgca tgtaattgcg
    27061 taattccgct gatgatgcca atcacgccac ccaccagcga tgtgatgccg gaaagcttaa
    27121 tagccagtct gtccagaccg agtaaaaaca gcgaagccag actggcaaac agatagagga
    27181 tcagtgacca ggttaataat tgcagggcat ccattagcgt tgctcctgag tgagggaaag
    27241 gggatcctcc ccgccattgg caaaccagct tgcggcaagg cgctgttttt ctttcatctg
    27301 acgttgcagg ctatcgtcgg tgatcagccg taacgcctga ttcgggcaag cgcgaacgca
    27361 ggccggacct tctggcaaga aatcacacag gtcgcatttc accgcgacgg tctggacacc
    27421 cggttcccag cgcagcaaag catgttgggt cggcgcgttt tcttcgccgt ctttgagtga
    27481 gccgtcagcc tgaaaaacat attgcgcagg ggcattcacc ggacggcttc ctgaagcgct
    27541 gattgcgcca aatgggcaga ccacggcgca aagcttgcag ccaatacaga ggctttcgtt
    27601 gagttggatc gcatcgtccc tctgggagat ggcattgacc gggcagacct gcaggcaagg
    27661 ggcttcctca cagtgatgac acacgacagg ggcagtgagt gttgacgtct tcgccagggc
    27721 caggcgcggg tgttgctgta aaccttgcgt tttatgcacg tccgaacagg cagcgagaca
    27781 ggtattacat cctgtacacc acagtggttc ggccaccaca aagcggttca tgtcggctcc
    27841 ttgaaaatga tatgaaacat tgcatttcat attgcataaa ggatgccagt ctgattaatt
    27901 taatgtattc aataagttat gaattaactg tattctgaac ggtacaaaaa tgatggtcat
    27961 taacgttcag caaaaaatga attcgacacc cgtgtcgcag gggaaagcga gaatgtgatc
    28021 ttgatcacga gaagtgaggt gtgctgaccg caagcgcagc cagcacggaa tggagcaaag
    28081 taatcaggcg tgttctttca gccagttcag cacaacgtcg tggtgattgc tggttttgaa
    28141 atcgtcaaag acatgttcga ttttgccatc agcgtcaatc aggaagctga tgcgatgaat
    28201 accatcgtag gttttgccca tgaaggactt ttcaccccag acgccgaatt gttcgcacac
    28261 ctggtggtcc tcatcagaca ggagcgtaaa gttaagcagc tctttttccg caaaacggga
    28321 gagtttttcg ggtttatcgg tgctgatacc cagcacatca acgcccgctt ttttcaactc
    28381 atccatgtta tcgcgtaagc cgcaggcctg tacggtacag ccgggggtca tggctttcgg
    28441 gtagaaataa accagaacac gctgtccctg gaagtcggtc aaattaactt gttctccgtc
    28501 ttgatccggc aagctaaatt tcggtgcgat atcaccggct ttcagtggat tcattactta
    28561 actccatcct gttcatcatg ttgggaataa ttgacgacgt taatactgcc ttgtgcattg
    28621 agttctgtac atagggcttt gaacgcttgc tcaatatttg ctgcgtccgc agatgcgggg
    28681 ctgtgggcgg ttatctgaat atgcaactgc gcagcccgtt cattttcagc aggttgcgtg
    28741 cgcgacacca gctccgcaat gttcatatga tgcgcgtcga aaagtgctgt gaagcgttca
    28801 attaaatgcg gggagtctgc cacatcgacc tgaacccaga cagatgctgg cattggcgga
    28861 cgcggacgcg ccgtcgtgcg cttcatcacg attaaaagat ccagttctgc acctttcaac
    28921 ggtaacgttg attcaatcag agtaatggca ttccatgaac cggaaagcag catgataaac
    28981 gtgaactctt ctcccagcat cgccaggcga ctgtcttcaa tattacagcc gcaactactg
    29041 acatgacggg tgatggtgtt cacaattcca gggcgatcgg cacccaacgc agtgatcacc
    29101 agataatgtt gcgatgacag tgtcaaactg gttattcctt taaggggtga gttgttctta
    29161 aggaaagcat aaaaaaaaca tgcatacaac aatcagaacg gttctgtctg cttgctttta
    29221 atgccatacc aaacgtacca ttgagacact tgtttgcaca gaggatggcc catgttcacg
    29281 ggaagtattg tcgcgattgt tactccgatg gatgaaaaag gtaatgtctg tcgggctagc
    29341 ttgaaaaaac tgattgatta tcatgtcgcc agcggtactt cggcgatcgt ttctgttggc
    29401 accactggcg agtccgctac cttaaatcat gacgaacatg ctgatgtggt gatgatgacg
    29461 ctggagctgg ctgacgggcg cattccggtg attgccggga ccggcgctaa cgctactgcg
    29521 gaagccatta gcctgacgca gcgcttcaat gacagtggta tcgtcggctg cctgacggta
    29581 accccttact acaatcgtcc gtcgcaagaa ggtttgtatc agcatttcaa agccatcgct
    29641 gaacatactg acctgccgca aattctgtat aatgtgccgt cccgtactgg ctgcgatctg
    29701 cttccggaaa cggtgggccg tctggcgaaa gtaaaaaata ttatcggaat caaagaggca
    29761 acagggaact taacgcgtgt aaaccagatc aaagagctgg tttcagatga ttttgttctg
    29821 ctgagcggcg atgatgcgag cgcgctggac ttcatgcaat taggcggcca tggggttatt
    29881 tccgttacgg ctaacgtcgc agcgcgtgat atggcccaga tgtgcaaact ggcagcagaa
    29941 gggcattttg ccgaggcacg cgttattaat cagcgtctga tgccattaca caacaaacta
    30001 tttgtcgaac ccaatccaat cccggtgaaa tgggcatgta aggaactggg tcttgtggcg
    30061 accgatacgc tgcgcctgcc aatgacacct atcaccgaca gtggtcgtga gacggtcaga
    30121 gcggcgctta agcatgccgg tttgctgtaa agtttaggga gatttgatgg cttactctgt
    30181 tcaaaagtcg cgcctggcaa aggttgcggg tgtttcgctt gttttattac tcgctgcctg
    30241 tagttctgac tcacgctata agcgtcaggt cagtggtgat gaagcctacc tggaagcggc
    30301 accgcttgcg gagcttcatg ccccggctgg aatgattttg ccggtgacct ccggtgatta
    30361 tgcaatcccg gtgaccaacg gtagtggtgc tgtcggtaag gcgctggaca ttcgtccacc
    30421 agcccagccg ctggcactgg tttctggcgc gcgtacccag ttcacgggcg ataccgcttc
    30481 attgctggtg gaaaatggtc gtggcaatac tctgtggccg caggtggtta gcgtgctgca
    30541 ggcgaaaaac tacaccatca cccaacgtga tgatgctggt cagacactga ccaccgattg
    30601 ggtacaatgg aaccgtctgg acgaagacga gcagtatcgt ggtcgttatc aaatctctgt
    30661 taagccgcag ggttatcagc aggcggttac ggttaaactg ctgaacctgg aacaggcggg
    30721 caaaccggtt gcagacgcgg cttccatgca gcgttacagc acggagatga tgaacgttat
    30781 ttccgccggt ctggataaat ctgccactga cgccgcgaac gctgcgcaaa atcgtgcctc
    30841 caccactatg gacgtacaaa gtgcagctga tgacaccggt ttaccgatgc tggtcgtacg
    30901 cgggccgttc aatgtggttt ggcaacgtct gccagcggcg ctggaaaaag tgggcatgaa
    30961 agtgaccgac agcacccgtt cgcagggtaa catggccgta acttacaagc cgctgtctga
    31021 cagcgactgg caggaactgg gcgcgagcga tccaggcctg gcatccggtg actataaact
    31081 gcaggttggc gatttagata accgcagcag cctacagttc atcgatccga aaggtcatac
    31141 tctgactcag agtcagaacg acgcgctggt agctgtcttc caggctgcgt ttagcaagta
    31201 aaaatacagg gctggagtca tccggccctt ttttctgata tgatacgcaa acgtgtgcgt
    31261 cggcaggaaa acgcgatttt agcggtaatt cgcactaaat ttgtttgtcg gacgtaggtc
    31321 ggataaggcg ttcttgccgc atctgacaaa acatccggca caccagacag caaaagattt
    31381 taaaacgtta attcacaccc aggagtgata aagatgcaaa agcaagctga gttgtatcgt
    31441 ggtaaagcga aaaccgtata cagcacggaa aacccggacc tgttggtgct cgaattccgc
    31501 aatgatacgt cagcagggga tggcgcgcgc attgagcagt ttgatcgcaa aggtatggtg
    31561 aacaacaagt tcaactactt cattatgagc aaactggctg aagcgggtat cccgactcaa
    31621 atggagcgtc tgctctccga taccgaatgt ctggtgaaaa agctggatat ggtgccggtt
    31681 gagtgtgtcg tgcgtaaccg tgctgctggc tctctggtga aacgtcttgg aatcgaagaa
    31741 ggtattgagc tgaacccgcc gctgttcgat ctgttcctga aaaacgacgc catgcacgat
    31801 ccgatggtca acgaatctta ctgcgaaacc tttggctggg tgagcaaaga gaacctggcg
    31861 cgtatgaaag agctgaccta caaagcgaac gacgtgctga aaaaactgtt cgatgatgct
    31921 ggtctgattt tggtcgactt taaactggag tttggcctgt acaaaggtga agtggtactg
    31981 ggcgatgagt tctccccgga cggcagccgc ctgtgggaca aagaaacgct ggagaaaatg
    32041 gacaaagacc gtttccgcca gagcctcggt ggcctgatcg aagcctatga agccgtcgcc
    32101 cgccgcctgg gtgtacagct ggactgattt ttctgttcat catcttgccg tgcagctggc
    32161 acggcaagac aaccgctctc ggaagatgtg cattgaacgc tcattcctcc gccatttcat
    32221 cccgctttcc tgtggtaatc ttcctctgaa ccacctacga tcatttctat aatgaatata
    32281 ttgttgaggt atctatgcgt tggcaagggc gacgtgaaag tgacaatgtt gaagacaggc
    32341 gcaacagctc tggtggtccg tcaatgggcg gtcccggttt tcgcctgcca agcggtaaag
    32401 gcgggctgat tttactgata gtcgtgctgg ttgcaggcta ctatggcgtt gatttaaccg
    32461 ggttgatgac cggacagccg gtttcccaac agcaatcaac acggtcaatt agtcctaatg
    32521 aagacgaagc cgcaaaattc acttcggtga ttctggcaac cacggaagac acctggggac
    32581 agcaattcga gaagatgggt aaaacctatc agcaaccgaa gctggtcatg taccgtggaa
    32641 tgacgcgcac cggctgcggg gcgggccagt ccattatggg accgttctat tgcccggcgg
    32701 atggcacggt ttatatcgat ctctctttct atgatgacat gaaagacaaa cttggcgcgg
    32761 atggcgattt tgcccagggg tacgttatcg cccatgaagt cggtcatcat gtgcagaaac
    32821 tgttaggcat cgagccgaaa gttcgtcaac tgcaacaaaa cgcgacgcag gcggaagtga
    32881 accgcttatc tgtgcgtatg gaactccagg ccgactgttt tgccggtgtc tgggggcata
    32941 gtatgcagca gcaaggtgtt ctggaaaccg gcgatctgga agaggcgctg aacgcggcgc
    33001 aggccatcgg cgatgaccgt ttacaacagc aaagtcaggg gcgagtagta ccagacagtt
    33061 tcactcatgg cacttctcag caacgctaca gctggtttaa acgtggtttc gacagcggtg
    33121 atccggcaca atgcaatact tttggtaaaa gcatttaact tccggggcag ggatggctga
    33181 actgactgcg cttcacacat taacagcgca aatgaaacgt gaagggatcc gccgcttgct
    33241 ggtgttgagc ggggaagagg gttggtgttt tgatcatgcg cttaagttgc gtgatgcctt
    33301 acctggcgac tggctgtgga tttcgccgca gccagtcgct gaaaaccact gttttccctc
    33361 ggcgctacaa actttacttg ggcgcgagtt ccggcatgcg gtattcgacg cccgccacgg
    33421 ctttgatgcc gctgcctttg cggcacttag cggaacgttg aaagcgggaa gttggctggt
    33481 tttgttactc cctgtatggg aagagtggga aaaccaacct gatgccgact cgctgcgctg
    33541 gagtgattgc cctgacccta ttgcgacgcc gcattttgtc cagcatctca aacgcgtact
    33601 tacggcgaat aacgacgcta tcctctggcg gcagaaccag cagttctcgt tggcgcattt
    33661 tgctccccgt actgactggc accccgctac cggcgcacca caaccagaac aacagcaact
    33721 cttacagcag ttactgacca tgccgccggg cgtggcagcg gtaactgctg cgcgtgggcg
    33781 cggtaaatcg gcgttggcag ggcaactcat ttctcgtatt gcgggcagtg cgattgtcac
    33841 cgcgcccgca aaagcggcaa cggatgtact ggcacaattt gcgggcgaga agtttcgctt
    33901 tattgcaccg gatgccttgt tagccagcga tgagcaagcc gactggctgg tggtcgatga
    33961 agccgcagcc atacctgcgc cgttgttgca tcaactggta tcgcgttttc ctcgaacgtt
    34021 gttaaccact acggtgcagg gctacgaagg caccggacgt ggttttttgc tgaaattttg
    34081 cgctcgcttt ccgcatttac accgttttga actgcaacaa ccgatccgct gggcgcaggg
    34141 atgcccgctg gaaaaaatgg ttagtgaggc actggtgttt gacgatgaaa acttcaccca
    34201 tacaccacaa ggcaatatcg tcattgccgc atttgaacag acgttatggc gaagtgagcc
    34261 agaaacgccg ttaaaggttt atcaattatt gtctggtgcg cactaccgga cttcgccgct
    34321 ggatttacgc cgcatgatgg atgcaccagg tcaacatttt ttacaggcgg ctggcgaaaa
    34381 cgagattgcc ggagcgctgt ggctggtgga tgagggcgga ttatctcaag aactcagtca
    34441 ggcggtatgg gcgggttttc gtcgcccgcg gggtaatctg gtggcccagt cgctggcggc
    34501 gcacggcagc aatccactgg cggcgacatt gcgcggacgg cgggttagcc ggatagcagt
    34561 tcatcctgct cgtcagcggg aaggcacagg gcgacagctt attgttggtg ctttgcaata
    34621 tacgcatgac ctcgactatc tttcggtgag ttttggttac accgaggagt tatggcgttt
    34681 ctggcaacgc tgcggttttg tgctggtgcg aatgggtaat catcgtgaag ccagcagcgg
    34741 ttgctatacg gcgatggcgc tgttaccgat gagtgatgcg ggtaaacagc tggctgaacg
    34801 tgagcattac cgtttacgtc gcgatgcgca agctctcgcg cagtggaatg gcgaaacgct
    34861 tcctgttgat ccactaaacg atgccgtcct ttctgacgac gactggcttg aactggccgg
    34921 ttttgctttc actcatcgtc cgctattaac gtcgttaggt tgcttattgc gtctgctaca
    34981 aaccagcgaa ctggcattac cggcgctgcg tgggcgttta cagaaaaacg ccagtgacgc
    35041 gcagttatgt accacactta aactttcagg ccgtaagctg ttactggtcc gtcaacgcga
    35101 agaagccgcg caggcgctgt tcgcacttaa tgatgttcgc actgagcgtc tgcgcgatcg
    35161 cataacgcaa tggcaatttt ttcactgact ccttcaattt tctcccatgg tcattgtgag
    35221 tcagcggcgt aaaattaatg acatcagtta aggagttcgc catgaaacat gaccattttg
    35281 ttgttcaaag cccggataaa cctgcgcaac agttgttact gcttttccac ggtgtcgggg
    35341 ataacccggt ggcaatgggg gaaataggca gctggtttgc gccgctattt cctgatgcgc
    35401 tggtggtgag tgtcggaggc gcggagccga gcggtaatcc ggcggggcgt cagtggtttt
    35461 cggtgcaggg tattacggaa gataatcgcc aggcacgtgt ggatgccatc atgccgacgt
    35521 ttattgaaac ggtgcgctac tggcagaaac agagcggagt aggggctaac gccacggcac
    35581 taatcggttt ctctcaaggc gcgattatgg cgctggagag cattaaagcc gaaccgggcc
    35641 ttgcttcgcg cgtcattgcc tttaacggac gctatgccag cctgccggaa accgcgtcta
    35701 cagccacgac aattcacttg attcatggtg gtgaagatcc ggttatcgat ctggcgcatg
    35761 cggttgcggc gcaggaagcg ttaatcagtg ccggtggtga cgtgacgctg gatatcgtgg
    35821 aggatttagg ccatgcgatc gacaatcgca gtatgcaatt tgccctggat cacttgcgtt
    35881 ataccattcc gaaacattat ttcgacgaag cgctaagtgg cggtaagcct ggcaatgatg
    35941 atgtgattga aatgatgtaa taataaccgg gtaggcctga taagactgta tcaggcatca
    36001 tggccggatg tggcgtgaac gccttatccg gccgctcagc ttacttcttc ggttgatcct
    36061 ttttcggcca gtcgtcgtca tcgtcccact tatcgttgaa atcacgatgc gggggaagct
    36121 ccggcttgtt ggcgagaaat ttcttatggt cgacgcgctt gagatcttta atcacgttca
    36181 gcaggacgcc taccaaaaag acaatcacca gaatccacca atatttagcc agccagtcca
    36241 tgcttatttc ctcttgcaga accactcatc aggcgacgag ctgttccatg atacgttgat
    36301 acatacgggc aagtagctgc aggtcggcag cgttcacaca ttcattaatt ttatgaatag
    36361 tggcattgac cggcccgagt tccaccacct gcgcccccat gcgggcaata aagcgcccgt
    36421 cggacgttcc gcctgtggtc agtagctgcg gtttaatttc attatagtgc tcaaccgcgt
    36481 taacgaccgc atccaccagt ttaccgcgcg cggtcaaaaa tggctgcccg gaaagccacc
    36541 attccaccgt ataacgcagt tgatgttttt caagcagggc aagtacctgc gctttgatca
    36601 tctcatcagt cagttcggtg ctgaagcgga agttaaactg cacaaacagt tcacccggaa
    36661 taacgttgtt actgcccgtt cccgcctgaa tattggcaat ctgcatactg gtcgccggga
    36721 agaattcatt gccctgatcc cactcaatag ccactaattc attaaggaaa ggtgctgcgc
    36781 gatgtaccgg attgtcagcc agatgtgggt aggcaacatg cccctgcacg ccatgaatgg
    36841 tgaggttgca ggttaacgat ccgcgacgac catttttcac cacatcacct accacttcga
    36901 tactcgacgg ttcgccaacc aggcagtaat cgagacgctc attacgtgcc atcaacgctt
    36961 cgacgacttt taccgtaccg ttatgggcac tggcttcctc atcagaagtg atcagaaatg
    37021 ccagtcgacc tgcatggttg ggatgttgtg cgacaaagcg ttctgccgct accaccatcg
    37081 ctgccagcga gcctttcata tctgccgcac cgcgcccgaa taacatgcca tcacgaatgg
    37141 tgggttcaaa cggcggattg atccaacgat cggcgtcgcc aggcggcacc acgtcggtat
    37201 gcccggcaaa ggccaacgtt tcaccttgcc cacgccatgc ccaaaaattc tgcgtatcgg
    37261 caaaatccat gcgttcaacg gtaaaaccga tcgcctgcaa acgttcaatc aacaaagcct
    37321 gacatcctgc gtcatcagga ctcagggaag ggcggcgaat aagctgttgt gtcagctcaa
    37381 taaccgggca cgacatagac tacacctcat ggaaaaattg ctgataactg gaatcactga
    37441 aacccagcag cataggctta ccgggcgtgc agagcaatgg acgtttgata attgcaggca
    37501 tttcagtcat taatgccgcc gcagaggccg catcgatgat tttattgcgg gtggtttcgt
    37561 ccagtttacg ccaggttgta ccacgggtgt tgagtaacgc ttcccagcct aattcgttga
    37621 taaaaccgtt caataattcg ctgtccagcc catcgacgcg gtaatcatga aaacgatagt
    37681 cgatgttatt ggcttccagc caacggcgag cctttttaat ggtgtcacaa tttttgatgc
    37741 cgtaaagtgt aaccataatg aatccttttt
//
LOCUS       DACBVI010000027        37368 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.027, whole genome shotgun sequence.
ACCESSION   DACBVI010000027 DACBVI010000000
VERSION     DACBVI010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.027"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            204..1577
                     /gene="aroP"
                     /locus_tag="IU325_06900"
     CDS             204..1577
                     /gene="aroP"
                     /locus_tag="IU325_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HAP5808770.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1620..2474)
                     /gene="ampE"
                     /locus_tag="IU325_06905"
     CDS             complement(1620..2474)
                     /gene="ampE"
                     /locus_tag="IU325_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HAP5808771.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKLETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2471..3022)
                     /gene="ampD"
                     /locus_tag="IU325_06910"
     CDS             complement(2471..3022)
                     /gene="ampD"
                     /locus_tag="IU325_06910"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HAP5808772.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVNKETT"
     gene            3110..4003
                     /gene="nadC"
                     /locus_tag="IU325_06915"
     CDS             3110..4003
                     /gene="nadC"
                     /locus_tag="IU325_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HAP5808773.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4207..4646
                     /gene="ppdD"
                     /locus_tag="IU325_06920"
                     /pseudo
     CDS             4207..4646
                     /gene="ppdD"
                     /locus_tag="IU325_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
     gene            4656..6041
                     /gene="gspE"
                     /locus_tag="IU325_06925"
     CDS             4656..6041
                     /gene="gspE"
                     /locus_tag="IU325_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HAP5808774.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPRWQAPGCRHCYHGF
                     YGRTALFEVLPITPIIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6031..7232
                     /gene="hofC"
                     /locus_tag="IU325_06930"
                     /pseudo
     CDS             6031..7232
                     /gene="hofC"
                     /locus_tag="IU325_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
     gene            complement(7267..8310)
                     /gene="guaC"
                     /locus_tag="IU325_06935"
     CDS             complement(7267..8310)
                     /gene="guaC"
                     /locus_tag="IU325_06935"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP5808775.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            8535..9155
                     /gene="coaE"
                     /locus_tag="IU325_06940"
     CDS             8535..9155
                     /gene="coaE"
                     /locus_tag="IU325_06940"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP5808776.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9155..9898
                     /gene="zapD"
                     /locus_tag="IU325_06945"
     CDS             9155..9898
                     /gene="zapD"
                     /locus_tag="IU325_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HAP5808777.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQTQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            9908..10105
                     /gene="yacG"
                     /locus_tag="IU325_06950"
     CDS             9908..10105
                     /gene="yacG"
                     /locus_tag="IU325_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HAP5808778.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10193..10591)
                     /gene="mutT"
                     /locus_tag="IU325_06955"
     CDS             complement(10193..10591)
                     /gene="mutT"
                     /locus_tag="IU325_06955"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HAP5808779.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMSE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVLAKLKRVYVG"
     gene            complement(10651..13356)
                     /gene="secA"
                     /locus_tag="IU325_06960"
     CDS             complement(10651..13356)
                     /gene="secA"
                     /locus_tag="IU325_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP5808780.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13418..13930)
                     /gene="secM"
                     /locus_tag="IU325_06965"
     CDS             complement(13418..13930)
                     /gene="secM"
                     /locus_tag="IU325_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HAP5808781.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14161..15078)
                     /gene="lpxC"
                     /locus_tag="IU325_06970"
     CDS             complement(14161..15078)
                     /gene="lpxC"
                     /locus_tag="IU325_06970"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HAP5808782.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15179..16330)
                     /gene="ftsZ"
                     /locus_tag="IU325_06975"
     CDS             complement(15179..16330)
                     /gene="ftsZ"
                     /locus_tag="IU325_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP5808783.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16391..17653)
                     /gene="ftsA"
                     /locus_tag="IU325_06980"
     CDS             complement(16391..17653)
                     /gene="ftsA"
                     /locus_tag="IU325_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017374018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP5808784.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17650..18480)
                     /gene="ftsQ"
                     /locus_tag="IU325_06985"
     CDS             complement(17650..18480)
                     /gene="ftsQ"
                     /locus_tag="IU325_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HAP5808785.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18482..19402)
                     /locus_tag="IU325_06990"
     CDS             complement(18482..19402)
                     /locus_tag="IU325_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase B"
                     /protein_id="HAP5808786.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19395..20870)
                     /gene="murC"
                     /locus_tag="IU325_06995"
     CDS             complement(19395..20870)
                     /gene="murC"
                     /locus_tag="IU325_06995"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP5808787.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEAQHD"
     gene            complement(20924..21991)
                     /gene="murG"
                     /locus_tag="IU325_07000"
     CDS             complement(20924..21991)
                     /gene="murG"
                     /locus_tag="IU325_07000"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP5808788.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAVKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQSQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(21988..23232)
                     /gene="ftsW"
                     /locus_tag="IU325_07005"
     CDS             complement(21988..23232)
                     /gene="ftsW"
                     /locus_tag="IU325_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HAP5808789.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23232..24548)
                     /gene="murD"
                     /locus_tag="IU325_07010"
     CDS             complement(23232..24548)
                     /gene="murD"
                     /locus_tag="IU325_07010"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP5808790.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGGLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24551..25633)
                     /gene="mraY"
                     /locus_tag="IU325_07015"
     CDS             complement(24551..25633)
                     /gene="mraY"
                     /locus_tag="IU325_07015"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP5808791.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGIIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25627..26985)
                     /gene="murF"
                     /locus_tag="IU325_07020"
     CDS             complement(25627..26985)
                     /gene="murF"
                     /locus_tag="IU325_07020"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP5808792.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALIARIKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(26982..28469)
                     /gene="murE"
                     /locus_tag="IU325_07025"
     CDS             complement(26982..28469)
                     /gene="murE"
                     /locus_tag="IU325_07025"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HAP5808793.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     IDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLAELLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCTGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28456..30222)
                     /gene="ftsI"
                     /locus_tag="IU325_07030"
     CDS             complement(28456..30222)
                     /gene="ftsI"
                     /locus_tag="IU325_07030"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HAP5808794.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMAEYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30238..30603)
                     /gene="ftsL"
                     /locus_tag="IU325_07035"
     CDS             complement(30238..30603)
                     /gene="ftsL"
                     /locus_tag="IU325_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP5808795.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30600..31541)
                     /gene="rsmH"
                     /locus_tag="IU325_07040"
     CDS             complement(30600..31541)
                     /gene="rsmH"
                     /locus_tag="IU325_07040"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP5808796.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31543..32001)
                     /gene="mraZ"
                     /locus_tag="IU325_07045"
     CDS             complement(31543..32001)
                     /gene="mraZ"
                     /locus_tag="IU325_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP5808797.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32603..33607)
                     /gene="cra"
                     /locus_tag="IU325_07050"
     CDS             complement(32603..33607)
                     /gene="cra"
                     /locus_tag="IU325_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HAP5808798.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33689..33775)
                     /locus_tag="IU325_07055"
     CDS             complement(33689..33775)
                     /locus_tag="IU325_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HAP5808799.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33787..34278)
                     /gene="ilvN"
                     /locus_tag="IU325_07060"
     CDS             complement(33787..34278)
                     /gene="ilvN"
                     /locus_tag="IU325_07060"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HAP5808800.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34281..36005)
                     /gene="ilvI"
                     /locus_tag="IU325_07065"
     CDS             complement(34281..36005)
                     /gene="ilvI"
                     /locus_tag="IU325_07065"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HAP5808801.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTSGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESVHQPLDEIRDWWQQIEQWRARQCLKYDSHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQIAHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            36125..36169
                     /locus_tag="IU325_07070"
     CDS             36125..36169
                     /locus_tag="IU325_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808802.1"
                     /translation="MKRNDKILHYRGLN"
     gene            complement(36324..37268)
                     /gene="leuO"
                     /locus_tag="IU325_07075"
     CDS             complement(36324..37268)
                     /gene="leuO"
                     /locus_tag="IU325_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HAP5808803.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         9009 a        10416 c         9114 g         8829 t
ORIGIN      
        1 ggtttctttt taagcaactc atcttcaacc atgcataaag cgggtgcatt cgctgccgca
       61 taccattatt cttgatctga cggaagtcct tttgtaacaa ttcaaacttc tttgatgtaa
      121 acaaattaat acaacaaacg gaattgcaaa cttacacacg catcactgcg tagatcaaaa
      181 aaacaatcac cgcacgaggt ttcatgatgg aaggtcaaca gcacggcgag cagctaaagc
      241 gcggccttaa aaaccgccat attcagctta tcgcgctggg tggcgcgata gggaccgggc
      301 tattcctggg tagcgcctcc gtaatacagt ccgcagggcc agggattatc ctgggttacg
      361 ccattgctgg ttttatcgcc tttctgatca tgcgtcagct aggtgaaatg gtggtcgaag
      421 aacctgtcgc aggctccttt agccactttg cttataaata ctggggcagt tttgccggtt
      481 tcgcctctgg ctggaactac tgggtactgt acgttttagt tgccatggca gagcttactg
      541 ctgtgggtaa atacattcag ttctggtatc cagaaatccc aacctgggtt tctgccgccg
      601 tattctttgt ggtgatcaac gccatcaacc tgaccaacgt aaaagtgttt ggtgagatgg
      661 agttctggtt tgccattatc aaagttatcg cagtggtagc gatgatcatc ttcggcggct
      721 ggctgctatt cagtggcaac ggcggcccgc aggcgaccgt tagcaacctg tgggatcaag
      781 gtggtttcct gccgcacggc ttcaccgggc tggtgatgat gatggcgatt atcatgttct
      841 cgttcggtgg tctggaactg gtggggatca ccgcagcaga agctgataac ccggagcaaa
      901 gtatcccgaa agcaactaac caggttatct accgcatcct gattttctat attggttcgt
      961 tagccgttct gctctcgctg atgccgtgga cccgcgttac tgctgatacc agtccgtttg
     1021 tactgatctt ccacgaattg ggcgatacct ttgtggcgaa tgcgctgaac atcgtggtac
     1081 tgactgctgc gctctccgtg tacaacagct gcgtatattg caacagccgt atgctgtttg
     1141 gtctggcaca gcagggcaac gcgccaaaag cgctggcgtc tgtcgacaaa cgcggtgtac
     1201 cggttaacac tattctggtg tctgcgctgg ttacggcatt gtgcgtgctg attaactacc
     1261 ttgcaccgga atccgcattc ggactgttaa tggcgctggt ggtatctgca ctggtaatca
     1321 actgggcgat gattagcctg gcgcatatga aattccgtcg cgccaagcag gaacaaggcg
     1381 tggtaactcg cttccctgct ctgctttatc cgctgggtaa ctggatctgc ctgctgttta
     1441 tggcggcggt actggtgatt atgctgatga ccccaggaat ggcgatttcg gtatacctga
     1501 tcccggtatg gctgatcgtg ttaggtatcg gctatctgtt taaagagaaa accgccaaag
     1561 ccgtaaaagc gcattaatct ctctacgccc tcacccatac agggtgaggg caataatctt
     1621 tacaccaacg ccccgtaaat cgtcagtagc gcaatcacca ccacgaccac gaacgaggtt
     1681 ttcttcgcca tggaaaccgc tgccttcggc gtctccagct tatcgacatg cggttcacgc
     1741 gccagagaga actgcgccag acgcgttaat acctgatatt gcgaagtatg gaaatcaccc
     1801 agcgaagcaa accaggccgg taacgctttc tccccatgac cgatcaaggc atataccaca
     1861 cccgcaagac gaaccggcac ccaatccagt acatgaagca cggcatcaat gccggactgt
     1921 aaacgatgat gcggcgtctg gtaacgcgcc agccagtatt gccatgcacg caaaaacgca
     1981 taccccatca gcgtaacggg tccccaggtt ccccccacaa tcagccagaa cagcggtgca
     2041 agataaaaac gaaagttaat ccacagcaat gcattttgca gctcacgcaa atactcacgt
     2101 tcatcgcagc ctgccgggac gccgtgaatc atggtgagtt cgccagccat cgtggcacgg
     2161 gcatggctat cattacgtga agcagctgtc agataagcat gataatgaag acgaacttta
     2221 cctgcgccaa tacacagcaa accaatcagc agccacacca gcagcgtggg aacgttgaac
     2281 aatactccct gcaatgcgcg taacagtaaa aaagtcacgc ccatcgcaat aatggtcatg
     2341 cctaatgtgc gcccgagaga aaaatgtttc acccgccgaa agaacgcttc aagacgatga
     2401 tcaagctgcc agtgctcgcc caacttaaac aggcgctcga aaattaacac cagtaaggtt
     2461 gtaaatagcg tcatgttgtc tccttgttga ccagcgcacg aaaccgtgcc caatcaaatg
     2521 caggaccggg atcggttttc cgatccggcg caatatcaca atggcccgtc atgttattag
     2581 cgatatctgg atagcgatca atcagtgccc gcgtaaccgc cgcaagctgt tgatactgcg
     2641 catcggtata cgccagcgta tcggtgcctt ccagctcaat cccaatagaa aaatcattgc
     2701 agcgttcgcg cccctgatac tgagagactc cggcatgcca ggcacgttta tcgaaaggaa
     2761 catactggac tatttcacca tcgcggcgaa tcaaacagtg agcggagacg cgcaaatggg
     2821 cgatctcagc aaagaaagga tgtgcctgcg gatcaatagt tccagtgaat aatgcgtcga
     2881 tccacggacc gccaaactcg cctggcggca ggctaatatt gtgcaccacc agcagggtgg
     2941 gtgtttcgtc atccgggcgg caatcgtaat gtggtgaggg aacgcggcgc gcgccaacca
     3001 gccacccctg ttctaacaac atgcaggatc tccttatatg tggtgctaat acccggttca
     3061 gagtagcatg tttctacctt atgattcgtt agctatctgg agttttaaca tgccgcctcg
     3121 ccgctataac cctgacaccc gacgtgacga gctgctggaa cgcattaatc tcgatatccc
     3181 cggcgcggtg gcccaggcgc tgcgggaaga tttaggcgga acagtcgatg ccaacaatga
     3241 cattacggca aaacttttac cggaaaactc tcgctctcat gccacagtga tcacccgcga
     3301 gaatggcgta ttttgcggca aacgctgggt tgaagaggtg tttattcaac tggcaggcga
     3361 cgatgtcacc ataatctggc atgtggatga cggcgatgtc atcaacgcca atcaaccctt
     3421 gttcgaactt gaaggcccat cccgcgtgct gttaacgggc gaacgcactg cgcttaattt
     3481 tgtgcaaacc ctttcagggg ttgccagtaa ggtacgccac tatgtcgaat tactggaggg
     3541 caccaacacg cagttgttgg atacgcgcaa aaccttaccc ggcctgcgtt cagctctgaa
     3601 atacgcggta ctttgcggcg gcggagcgaa tcaccgtctg gggctttctg atgcctttct
     3661 gatcaaagaa aaccatatta ttgcctccgg ctcagtgcgc caggcggtcg aaaaagcatc
     3721 ctggctgcac ccggatgcgc cagtagaagt cgaagtagag aatctggaag aacttgatga
     3781 agccctgaaa gcaggtgccg atatcatcat gctggataac ttcgaaacag aacagatgcg
     3841 cgaagccgtc aaacgcacca acggcaaggc gctactggaa gtgtctggca acgtcactga
     3901 caaaacactg cgcgaatttg ccgaaacggg cgtggacttt atctccgttg gtgcactaac
     3961 taaacacgta caggcactcg acctttcaat gcgttttcgc taattgctaa cagatttatc
     4021 cgctccatcg cgggcggata aatctcccct tccccaactt tcttcgtaac gcctcgcaaa
     4081 tttcttgtct ttcgctgcaa cgaaataaaa aacctcggaa gctgctttcc cgtttcgttt
     4141 tttgcccctc gcttgtgctg atggcgtttg ccaaagtagc gccaaccaaa tcaaggagcg
     4201 aaacagatgg acaagcaacg cggttttaca cttatcgaac tgatggtggt tattggcatc
     4261 attgccattt taagcgccat tggtattccc gcttatcaaa actacctgcg caaagccgca
     4321 ctcaccgaca tgctacaaac ctttgtgcct tacctaccgc cgtagagttg tgcgcgctgg
     4381 aacatggtgg attaaatacc tgcgacagtg gcagcaatgg cattccctcg cctaccacca
     4441 cccgctatgt ttcagccatg agtgtggcaa agggcgtggt gtcgctgacc gggcaagaaa
     4501 gtctcaatgg gctaagcgtc gtcatgacac cgggttggga taacgcaaac ggcgtcaccg
     4561 gttggacgcg caactgcaat attcaaagtg acagcgcatt gcagcaagcc tgcgaagatg
     4621 tcttccgttt tgatgacgcc aactaaggag cggcaatgaa tattccacag ctcaccgccc
     4681 tgtgtctgcg ttatcaggga gtcttgctgg atgccagcga agaggtggtt catgttgcgg
     4741 tggtcgatgc cccttcgcat gagctactgg acgcattgca tttcgctacc accaaacgta
     4801 ttgagatcac ctgctggacg cgccaacaaa tggaaggtca cgccagtcgc acacaacaga
     4861 cattgcccgt agctgttcag gagaagcatc agcccaaagc agagttgctg actcgaacgt
     4921 tacaatctgc gctggaacaa cgcgcgtctg atattcatat cgaaccagcg gacaatgcct
     4981 accgcatccg cttgcgtatc gacggcgtat tgcatccttt accggatgtt tcaccggatg
     5041 ccggagtcgc attaaccgcc agattaaaag tgctgggcaa cctggatatt gcggaacatc
     5101 gcctgccgca ggacgggcaa ttcactgtcg aactggcagg aaacgccgtc tcatttcgta
     5161 ttgcgacctt accatgtcgg ggtggtgaaa aggtggtatt aagattgtta cagcaggtga
     5221 gtcaggcact ggatgtcaac acgcttggaa tgcagccgtt acaactggcg gactttgctc
     5281 atgccttgca acaaccacag ggactggtgc tggtaacagg ccctacaggc agcggcaaaa
     5341 cggtcacgct ttatagtgcc ctgcaaacgc tgaatactgc tgatattaat atttgtagcg
     5401 tcgaagatcc ggttgagatc cccatagccg gactaaacca gacgcaaatc catccgcgtg
     5461 ccggtcttac ctttcagggc gttttgcgtg cgttattgcg ccaggaccct gacgtcatta
     5521 tgatcggaga gatccgcgat ggcgaaacag cagagatcgc tattaaagcg gcgcaaaccg
     5581 gtcacctggt gttgtctacc ctacacacca actccacctg cgaaacgctg gtgcgtttac
     5641 agcaaatggg cgttgctcgc tggatgctct catcagcgct tacgctggta atagcccagc
     5701 gtctggtacg caaactttgc ccacattgtc gccgacagca aggggagcca atccatattc
     5761 cagacaatgt atggccgtcg ccgctgcccc gctggcaagc ccctggttgt agacactgct
     5821 accacggttt ttatggtcgt acggccttat ttgaagttct acccataaca ccgatcatac
     5881 gccagcttat ttccgctaat accgacgttg aatcgctgga aacacacgcc cgacaggcgg
     5941 gtatgcgtac gctttttgaa aacggctgcc tggccgtgga gcaaggctta accacctttg
     6001 aagagttaat ccgcgtattg gggatgccgc atggcgagta agcaactctg gcgctggcat
     6061 ggcataaccg gcgacggcaa tgcgcaagat gggatgctat gggctgagag ccgtgctttg
     6121 ctgctcatag cactacagca acagatggtt accccactta gcctgaagcg aatcgccatc
     6181 aattctgcgc agtggcgagg agataaaagc gcggaagtca ttcatcaact ggcgacgcta
     6241 ctcaaagccg ggttaacgct ttctgaaggg ctggcactgc tggcggaaca gcatcccagt
     6301 aagcaatggc aagcgttgct gcaatcgctg gcgcacgatc ttgaacaggg cgttgccttt
     6361 tccaatgcct tattaccctg gtcagaggta tttccgccgc tctatcaggc gatgatccgc
     6421 acgggtgaac tgaccggtaa gctggatgaa tgctgctttg aactggcgcg tcagcaaaaa
     6481 gcccagcgtc agttgaccga caaagtgaaa tcagcgttac gttatcccat tatcatttta
     6541 gcgatggcaa tcatggtggt tgtggcaatg ctgcatttgt tctaccggag tttgccgcta
     6601 tctataagac ctttaacact ccgctaccgg cgctaacgca ggggatcatg acgctggcaa
     6661 gctttagtgg cgaatggggt tggttgctgg tgctattcgg ctttctgctg gcgatagcca
     6721 ataagctgct gatgcgccac ccaaccaggc ttatcgcacg gcagaaattg ctgttacgca
     6781 tcccgattat gggttcactg atgcggggac aaaaactcac acagatcttt acgattctgg
     6841 cgctgacaca aagtgcaggc attacttttt tacagggcgt agagagcgtc agagaaacaa
     6901 tgcgctgccc gtactgggtg caacttctga cacaaatcca gcacgatatc agtaacggtc
     6961 aacccatctg gctggcgcta aaaaatgccg gtgagtttag cccactctgt ttgcaattag
     7021 tgagaacagg agaggcatcc ggctcgctgg atctcatgtt agacaacctc gcccatcatc
     7081 atcgggataa cacactggcg ctggcggata acctcgcagc cttactggaa ccggcgttgc
     7141 tgatcataac gggaggaatt atcggtacgc tggtggtggc aatgtatctg cccattttcc
     7201 atttaggcga tacaatgagt ggaatgggat aacgctggcg tgttgctcca cgccagcgtt
     7261 gggagattac aggttgttga agatgcggtt ttcttgttcc agcacacgga taaatgtggt
     7321 gcgcttggtc agctctttca ggcgtgaagc cccaacgtat gtacaagctg aacgcaggcc
     7381 gcccaaaata tcacgcgcgg tattttcaac cgggcctcgc agcggcagct taacggtttt
     7441 accttctgct gcgcgatatt ccgcaacgcc gccaacgtga cgtttcatcg cagactcgga
     7501 gctcatgccg tagaacagca taaatttctc gccgttctcc tcaacgatgc gaccgccgct
     7561 ctcttcgtgg cccgccagca tgccgccaag catgacgaaa tcggcaccgc cgccaaaggc
     7621 tttcgcgaca tcgcccggcg tggtgcagcc accgtcgctg acgatcattc cgcccagacc
     7681 atgcgcagca tcggcacatt cgatcaccgc agaaagttgc ggataaccga cgcctgtttt
     7741 gacgcgagtt gtacaaacag aacctgggcc aatgccaact ttaacgatat cagcaccgga
     7801 gaggataagc tcctcacaca tttcaccagt cactacgttg ccagcacaaa tggttttggt
     7861 cggccacgct tcacgcgctt tcgcaacgaa ctgcacgaag tgttcggaat aaccattcgc
     7921 cacgtcaata caaacgaaat ttaatgccgg gttcaggtcg agaatctgtt tagttttttc
     7981 gaaatccgca tctgatgtac cggtagaaac catcacatgt ttcagcacat cagcggaaga
     8041 attgttgata aacgcttgcc actcttcgac agaatagtgt ttatgcacag cagtcaaaat
     8101 atcaaaagaa gccagcgcag aggccataga aaatgtgcct acggtgtcca tatttgcggc
     8161 gataatcggc acgccggacc agctctgacc tgaatgtttg aaggtgaatt gacgttccag
     8221 ttcaacatcg gaacggcttt taagagtgga gcgtttaggg cggatgagaa cgtctttaaa
     8281 acctaacttc agatcttctt caatacgcat gtgcggattc ctggggttaa tggctataag
     8341 ggtaagagcg caactccagt gacgttatca tacgcactaa tcaatgcggc gcaagactgc
     8401 gaaattgtct ttttttacgc tacaatccca taaatttacc tggtaaatag tgagcgaacg
     8461 tttcatcaat ttcgctttcg tgcgctcatt tttaacgttt tgataatcat gatgaattcc
     8521 cgggaatata aattatgagg tatatagttg ccttaacggg aggcattggc agtggcaaga
     8581 gtaccgttgc caatgcgttt gctgatctcg gaattaacgt cattgatgcc gatattattg
     8641 cgcgtcaggt ggttgaacca ggcgcacctg cgctacatgc cattgctgat cactttggcg
     8701 ctaacatgat tgctgctgat ggaacattgc agcgccgggc cttgcgcgag cggatcttcg
     8761 ccaacccgga agagaaaaac tggcttaacg ccctgctgca tccgctgatt cagcaagaga
     8821 cgcaacacca gatccagcaa gctacttccc cctatgtact atgggttgtg ccattgctgg
     8881 tagaaaactc actgtataaa aaagcgaatc gagtgctggt ggtggatgtc agcccagaaa
     8941 cgcaacttaa gcgcaccatg cagcgcgatg atgtaactcg cgagcatgtt gaacaaatcc
     9001 ttgctgctca ggcaacgcgc gaagcccgcc ttgccgtggc agatgacgtc attgataata
     9061 acggcgcacc ggatgctatc gcatcggatg ttgcccgcct gcacgcacac tatttgcagc
     9121 ttgcgtcgca gtttgtctca caggaaaaac cgtaatgcag acccaggtcc tttttgaaca
     9181 tccactaaat gaaaaaatgc gtacatggct gcgcattgag tttttgattc agcaactcac
     9241 cgttaatcta cccatcgttg accacgctgg cgcgctgcat ttcttccgta atgtcagtga
     9301 attactggat gttttcgagc gcggcgaagt ccgcactgag ctgttgaaag aacttgaccg
     9361 gcagcaacgt aaactccaga cctggattgg cgtgcctggc gtggaccaga gccgtattga
     9421 agcattaatt cagcagttaa aagcggcggg gagcgtatta atttccgcgc cgcgtatcgg
     9481 gcaatttctg cgtgaagatc gtttgattgc tctggtgcgt cagcgactga gcatcccagg
     9541 cggctgttgc agctttgatt tacctacatt gcacatttgg ctgcatctac cccagacgca
     9601 gcgcgacagc caggtagaaa cctggattgc cagcctgaac ccgctcaccc aggcacttac
     9661 catggtgctg gatttaattc gccagtcggc ccccttccgt aaacaaacca gcctgaatgg
     9721 tttttatcag gataacggtg gcgatgccga cttgctgcgt ctgaatctgt cgctcgattc
     9781 acagctttat ccgcaaattt ccggtcataa gagccgtttt gccattcgtt ttatgccgct
     9841 ggacagtgaa aacggacagg taccggaacg tctggatttc gaactggcct gttgctaagg
     9901 agtaaaaatg tcagaaacta ttacggtgaa ttgcccaacc tgcgggaaaa cggtggtgtg
     9961 gggcgaaatc agcccgtttc ggccattttg ttccaaacgt tgccagctga tcgacctcgg
    10021 agaatgggct gctgaagaaa aacgaatccc cagcagtggc gatctttccg aaagcgatga
    10081 ctggagcgaa gagccaaagc agtgacattt tgtctgatgc gacgctggcc tgataagacg
    10141 cgccagcgtc gcatcgggca tcttgtgcaa atgttggatg cggcataaac gcctatccga
    10201 catatacacg cttaagcttc gcaagtaccg gttcattggc tggcggaaaa tcatcggcat
    10261 tcagaccgac cagcgacatc cactcaccgg gttgcccttc tttaccccac ggctcccctt
    10321 cccagctttc gaccagccag aaccacagag ttatatgcct gtccgggaat tcatattcca
    10381 gtttttcaaa tagcgaaaaa tgttgcgggg taatcccgac ttcctcctga agttcacgca
    10441 ccaccgcctg ttccggcgtt tcgctcattt caattttacc gccgggaaac tctagtttat
    10501 tcgccatgtg cgcatctgct gcgcgacggg ttataaagat ttcattgttc tcgttgcgaa
    10561 taatacctac cgcaatttgc agctttttca ttgtcttaaa cctataaaaa aggcgcagaa
    10621 tcctgcgcct tttacttcaa ctgttagctt ttattgcagg cgaccatggc actgcttgta
    10681 ttttttacca gaaccgcacg ggcaaggatc gttacgtcct actttgcgct caccggtttg
    10741 cgccgccagt gcagctgcag ctgcagagtc gtcatcctga tggctaagct gctgcatctg
    10801 cgctaaacgc tcggcttcca tacgacgctg ttgttccagc tcctcaacct cttcaggcat
    10861 acgtacctga actttgctca gcgtactgat aacttcatat ttcaacgact ccagcatcgc
    10921 tgcaaacatg gagaacgatt cacgtttgta ttcctgcttc ggatctttct gtgcatagcc
    10981 acgcaggtgg ataccctgac gcagatagtc catcgccgcc aggtgctctt tccacagaga
    11041 gtcaagcgtt tgcagcatga cgcctttttc gaagtgacgc atcatctctg caccaaccac
    11101 ttcttcttta cgctgataca cttcgatgga ctgcgccaga atgcgctcac gcagcgtctc
    11161 ttcatgcagt tctggttctt tatccagcca ctcggcaatt ggcaaatcaa ggtcgaagtc
    11221 gttcttcaga cgttcctgca gccccggaat atcccacatt tcttccagcg actgtggcgg
    11281 aatgtaggca tcaatggtcg ctttgaacac atcttcacga atgctgttga tggtttcact
    11341 cacatcgctg acatccagca gttcgttacg ctgggagtaa atggcgcgac gctgatcgtt
    11401 agccacgtca tcatattcca gcagttgctt acgaatgtcg aagttacgac tttcaacttt
    11461 acgctgggca ttggcaatcg ctttagtcac ccacgggtgt tcaatggctt cgcctggctt
    11521 catacccagt ttacgcatca tgccggatac tcggtcggaa gcaaaaatac gcatcagcgc
    11581 atcttccatc gacaggtaga aacgggaaga accagcatcc ccctgacgac cagaacgacc
    11641 gcgcaactgg ttatcgatac gacgggattc gtgacgctcg gtaccgatga tatgcaggcc
    11701 acctgctgcc agtaccgcat cgtgacgtac ctgccagtcg gctttaattt tttcaatttg
    11761 ctctgcggtc ggattttcca gcgcggcaac ttctgcctgc cagctaccac cgagcacaat
    11821 atctgtacca cgacccgcca tgttggtcgc gatagtcacc gcagccggat aacctgcctg
    11881 agcaacaatc gccgcttcgt tggcgtggaa tttggcgttc aggacgttgt gcttaatacc
    11941 ggctttggtc agttcgtttg ataccagctc cgatttttcg atggagatag tacccaccag
    12001 caccggctgg cctttcgcag tacgttcttt gatatcttca atgatcgcct gaattttttc
    12061 cgcttcagtc atgtagacca ggtccggcag atctttacga atcattggac ggttggtcgg
    12121 aacaacgacg gtatccagct tgtagatgga gctgaattcg aaagcttcgg tatcagcagt
    12181 accggtcatc cccgccagtt tttcatacag acggaagtag ttctggaagg tgatcgaagc
    12241 cagcgtttgg ttttcgttct ggatctgcac accttctttc gcttccacag cctggtgcag
    12301 accatcggac cagcgacgcc cctgcatggt acgaccggtg tgttcgtcaa cgatgataac
    12361 ttcaccatct ttaacgatgt aatcgacgtc gcgggtaaac agcgcatgag cgcgcagcgc
    12421 cgccgttacg tggtgcatca gcatgatgtt tgccggagag tacagagact ccccttcatc
    12481 catgatgcct tctttcacca gcagttcttc aatcaggacc agaccacgtt cggtcaggtt
    12541 cacctgacga gatttttcgt ccaccgagaa gtggccttcg ccctggaagg tttcggagtc
    12601 ttctttttcc tgacggatca ggtgcggaat aattttattc acgcgtttat acatttccga
    12661 gctgtcttct gccgggccgg aaatgatcag cggtgtacga gcttcatcga tcaggatgga
    12721 gtccacttcg tccaccagcg catagtgcag tttacgctgt acacgttctt cagggctgaa
    12781 cgccatgtta tcgcgcaggt agtcaaagcc gtattcgttg ttcgtaccgt aagtgatgtc
    12841 agctgcgtaa gcttcacgct tcgccggtgc tggcatgccc ggcaggttga taccgacagt
    12901 caggccaagg aattcaaaca gcggacggtt gttttcggcg tcacgttgcg ccaggtagtc
    12961 gttgacggta acaacgtgta cgcccttacc ggttaatgcg ttcaggtaag caggcagcgt
    13021 tgcggtcagg gttttacctt caccggtacg catttcggcg atgcagcgtt cgttaagaac
    13081 cataccgccg agtaactgaa cgtcgaagtg acgcatacca aagacgcgct tacttgcctc
    13141 acgcaccacg gcgaaagctt ccgggatcag attttccagc acttcgcctt tttccagacg
    13201 tgcacgaaac tctgcggttt tccctttcag ttcttcgtcg gagagttttt ccatctccgg
    13261 ttccatggca ttgatgatgt taaccacttt gcgcatccgg cgcagggtgc gatcgttacg
    13321 actaccgaaa actttagtta ataatttgat tagcataata aaatctcaaa cgccccgcgt
    13381 tgcggagtca ataaaatgaa gtaaaggttt attgttgtta gctgaggcgt tgagggccag
    13441 cacggatgcc ttgcgcctgg cttatccaga cgggcgtgct gaattttgct tgcggggtaa
    13501 aatgcgcata atcaatgcga taaccctttt cagacggcgt gccttcctgg gtcaacagcg
    13561 cgctgagcgt atccagtaat gcaagatgtt gcgcctgaag aggcaaagat tcttcagcaa
    13621 cgggcagcgt ttgcggtgcc attgcgaagg aaagatgacg gattaccgtg cgaatggcat
    13681 gttgatgcca gtaatcaacg gaatagttcg aattcgggcg gcgcgtgttc gcttccagca
    13741 aggccaattg accaaagtta actttggctg aaggctcgtg gttgcgggtt gtcgcttttg
    13801 cgggcgcgtt tggttcggcg gcgttgctga gcgcaggcaa acctaaactc gccgcgacca
    13861 tccctaataa gagatgcggc cagaagtagc gtttaccaaa ctgtcgccag cgcgtcagta
    13921 ttccactcac gttattgcca tcccgatcgt attcaaacgt atttagcgca caaatcttat
    13981 cattaaagcc tgaagtccac agcacgaatg aagataagtt gtgcgtaaaa gtgcttaaga
    14041 aaacagcgtt cgcaccgttt tttatcgttc ttataaaatt caaaaaaaaa cgactggttc
    14101 acctggccgg agagagtgcc agatttgcca gtcgaatttt atacgacagt ataaatgtcg
    14161 ttatgccagt acggctgaag gcgctttgaa ggccaacggc agttctgcgt cgtcctggaa
    14221 ggtcacatat tcccaggctt cctgttttgc aaggacagcc tgcagcagtt tgttattcag
    14281 tgcatgacca gatttataag cggtaaatgc accaataata ttgtgaccac acatgaacaa
    14341 gtcaccgatc gcatcgagca ttttgtgacg cacaaattcg tcttcaaaac gcaggccgtc
    14401 ttcgttcagt acgcgataat cgtcaacaac gatggcacaa tcgaagctgc cgcccaggca
    14461 caaaccacgg gactgcagat attcgatatc acgcatgaaa ccgaacgtac gcgcacggct
    14521 gatctggcgc ataaacgcat cagcggagaa gttcatcgca tagcgctggt tgctggaatc
    14581 aatagccgga tggttaaaat cgatggtgaa atccagcgaa aaaccattgt acggcttaaa
    14641 ttcagcccac ttatcgccat cttcgacacg aacagtctct ttgatgcgaa caaatttctt
    14701 ggcgcagttc agctcgtcga taccggcgtc aagcagcagg tatacaaacg gagcggcgct
    14761 gccgtccatg atcgggattt ccggcgcgtt aacttcgata acaatgttat cgatgcccaa
    14821 gcccgcgaga gcagcattga ggtgctctac ggttgaaatc cgtacatcat gctcgttgac
    14881 cagacacgta cagagcatgg tatcacgcac agatttggca tcggccggga aatctaccgg
    14941 tggattcaag tcggtgcgac gatagatgac cccggtgttg gccggcgcag ggcgtaacgt
    15001 cagggtgact ttcttgccgg tatgtaaacc gacacccgtc gcctgaacga tacgtttaag
    15061 tgtcctttgt ttgatcatcg tattatctcg ccaaattacc tatccaaccg aagtgtacta
    15121 tacattcggc gggccagttt agcacaaaga gcctcgaaac ccaaattcca gtcaattctt
    15181 aatcagcttg cttacgcagg aatgctggga tatccagata atctggctct ttcgcagttt
    15241 gcggcgcatt gtcattcacg actttagcaa ccggcttctg ctcctgggtc agcggagcca
    15301 tcccgtgctg ctggtagcga tccatcactg gctgctgaac ctgcttattg gtcaccagag
    15361 tgatttcagg acgtttgtcc atgccgatac ctgtcgcaac aacagttacg cgcagctcgt
    15421 cattcatatc cgggtcaaga gaagtaccga taaccacagt cgcgttgtcg gaagcaaatg
    15481 cacggatggt gttacctacc gtttcgaact catccagacg caggtcgaag cccgccgtga
    15541 tgttaaccag cacgccgcgt gcgccagaca ggtcgatatc ttcaagcagc ggagaagaga
    15601 tagccatttc agcagcttct tccgcacggt cttcaccgct cgccacgcca gaacccatca
    15661 ttgcgtagcc catttcagac attacggtgc gtacgtctgc aaagtccacg ttcatcaaac
    15721 ccggacgagt aatcagttcg gcgatacctt gcacagcgcc tttgagtaca tcgttcgctg
    15781 cgccaaacgc atccagcagg gagataccgc ggcccagaac tttcagcagt ttgtcgttcg
    15841 ggatagtgat cagagagtcc acatgtttgg acagttcagt gatcccctgc tccgcgaatg
    15901 ccatacgctt cttgccttca aagttgaaag gcttagtgac gacagcaacg gtcaggatac
    15961 ccaaatcttt tgccacttca gcgacgactg gagctgcgcc tgtaccggta ccaccaccca
    16021 tacccgcagc aataaagacc atgtctgcac cttccagcgc cgcacgcagt gcatcgcgat
    16081 cctcatcagc cgcattacgg ccaacttctg gattagcgcc agcgcccagt cctttggtga
    16141 taccgctacc gatttgaatc gtctgtccaa ccgctgtttt acgcagcgct tgtgcatcgg
    16201 tatttaccgc gaagaattca acaccttcaa tgcgctcgcg caccatgtgt tcaacagcat
    16261 taccgccgcc gccgccgacg ccgatgactt taatcaccgc gtcattggta agttccattg
    16321 gttcaaacat agtttctctc cgatttgtgc ctgtcgcctg aggccgtaat catcgtcggc
    16381 ctcataaaaa ttaaaactct tttcgcagcc aactattgag tcgcttgatc cacgagccaa
    16441 ctgatgctgt aacacgtttt tctacttcag cttcaccgtt aagatgtgac tcttttccat
    16501 agtgaagcaa tcccaccgcc gtcgaataat acggctcctg agcataatcc gttaaaccgg
    16561 taatgttcag cggcgcgccg atacgcactt gcgtatgaaa cacgcgctga gcacaggctg
    16621 caagaccttc gatctgcgct gcgccaccag ttaatacaat gcctgccgcc aggtgatgtt
    16681 taaccccttg ttggcgaagc ttttcctgca actgcaatat ctcttcgttg accaggttga
    16741 gcagctcggt atagcgcggc tcgatcacct ctgccagggt ctgacgttgc agactccgcg
    16801 gcggacgacc gcctacgctc ggcacttcca cgctctcatc ttttccaacg atggaaccca
    16861 gcgcacaacc gtggcgaact ttaatcgctt cggcgtcgct tggcggcgtg ccaaaggcgt
    16921 aagcgatatc actggtcacg acattgccag cataaggaat taccttagtg tggcgcaatg
    16981 ccccaccggt ataaacggcg atatccattg taccaccacc gatatcgacg acgcagacac
    17041 ccagttcacg ttcatcttcc gtcaataccg aataacttga tgccagtccg gcaaatatca
    17101 gttggtcaac tttcagccca caacgttcaa ccgctttgac gatgtttttc gccatatcgt
    17161 tgtgacatgt gatcaggtgc acttttgcct gcatccgcac gcccgaaagt cctaccggat
    17221 tcttgatccc ttcctgatag tcaatcgcat actcttgcgg gatcacatgc agcacacgat
    17281 gctcatcgcg cacacgcacc gatttcgcgg tatggacgac gttttccaca tcttcttgcg
    17341 tcacttcttc ttcagaaata ggcaccatac caatttcatt ctggcagctg atgtgcttac
    17401 cagaaagcgc cagatatacc gaagagatct gacaatctgc catcaattct gcctggtcaa
    17461 tggcacgttg tacgcacttg accacggatt cgaggtcgtt caccccgcct ttatccatac
    17521 cacgtgacgg gcagctgccc acgccaatga tattgaccat accgtcgggc agaacttccc
    17581 ctactaaagc ggcaaccttc gcggtaccaa tctccagtcc tactaccagt tttctgtccg
    17641 tcgccttgat cattgttgtt ctgcctgtgc ctgattttgt tgctgagtag attcctctgg
    17701 cggcaagggc gcccagccta ctgccgctcc agagtcataa cgcaaatcaa cgtagctaat
    17761 ccgtttgcca tcggtttgcg cctgctgctg taaaaccgga taaagttcta caaagcgagc
    17821 caaacgtttc atcgtatcgc cccggccaag attgagctta atatcgttat tcagcgtcaa
    17881 ctgccaggaa cgccgcgcgg tcatcgccgc ttccttcaga gtaaatctgt cctttgccag
    17941 catctgcccc atttcgcgat agccctgcaa cacttcatta gcgctgcctt ccgggccata
    18001 cagcattgga agcacctgct tgctggtgcg atctggcggc acgctgaagg tgtttccttc
    18061 cgcgtctacc atgtgttgat cattccaccg cgcaatcggc acatattcaa ccagatgaat
    18121 cttcaattca tcaggccact gctttctgac gctcacctgc ttaatccacg gcaggcgttg
    18181 ttctatttgc gtctggatga tgttgacatc ctgggtcata aaggtacccg gctcacccaa
    18241 tgccaggatc gactgccgga tatcgtcatt acgcgtgtaa tggcgttcac cggtcaacac
    18301 cagctttgag agcggcaggc gttgcgcatc ttccatccag cccaacacga cccagccgct
    18361 caccaacact gtcgttaaaa cggtcagcag gaaaaggatc cccgccagac gcgttccatt
    18421 attgcggcga gaagaaacct cttcttcgct gtttcgcgtg ttcagagcag cctgcgacat
    18481 attagtccgc cagttccaga attcgtacta ccaactgcga gaagctcata cctgcctgac
    18541 gtgccgccat cggcaccagg ctgtggctgg tcatacccgg tgaggtattg gcttccagca
    18601 gataaaactg tccatcgctg tccagcataa cgtcaatacg tccccatcct ttgcaaccta
    18661 acgtcgtcca tgctttcagc actaatgcct gcaaattggc ctcttgtgac gcttccagac
    18721 ctgcggggca gaaatactgt gtctcatcag agagatactt cgcctcataa tcatagaagg
    18781 ttccggacgg ttgaatacgt attgacggta aaatttcttc accgagtatc gcaaccgtga
    18841 actccggccc acttagccat ttttcaatca atacttcttc atcgtgctga aatgccaatc
    18901 ttaatgcatc ttgtagagca ttttctgcta ctacttttga cattcccaca ctggaacctt
    18961 cgcggctcgg cttaacgata accggcaaac ctaaggcaga aatttctgct aactgcttat
    19021 cactcaggcc tttttcaaac tctgcgcggg ttaacgctac ccacggagcg accggtaaac
    19081 cggcaccttg ccacagaagt ttgctgcgta gtttatccat tgaaagcgca gatgccatca
    19141 ctccgctgcc ggtataaggc aagcccatca gctcgagcat gccctgcagc gtgccatctt
    19201 caccgccgcg accgtgtagc gcgataaaca ctttctgaaa gcccatcgac ttcagttgcg
    19261 tcacgtcgac ttctttcggg tcgacaggat acgcgtcaat accgccttca cgcagcccgg
    19321 ctaacaccgc tgcgccagaa ttcagagaaa cttcccgctc agcggaggtc ccacccaaca
    19381 ggaccgcgat tttatcagtc atgttgtgct tcctccggag tttgcggctt cagtttgatt
    19441 tcagctaaag aacgggcaat ttttccaata ttaccagccc cctgaacgag aatcaggtcg
    19501 ttaccggtta ataccggtgc cagcatctcg gctacctgcg ccgggtcagg caccagaatg
    19561 ggatcaattt tcccacgtcc acgaattgtg cgacacagcg aacggctgtc cgctcccgga
    19621 attggcgctt cgccagccgg atacacttcc agcatcaaca gggtatcaac ctgcgtcagc
    19681 acattggcga aatcatcata caggtcgcgt gtacgggtaa aacggtgcgg ctgaaacagc
    19741 attaccaggt ttttatccgg ccagcctgca cgcgccgctt taatagtggc gtccacttcc
    19801 gtcgggtggt ggccgtagtc atcgaccaac attgccgtac cgcttttacc attcactggc
    19861 tccagcggga attcaccgag gaaatcaaaa cgacgaccag taccctggaa gctctccagc
    19921 gcacgcaaaa tagcctcgtc gtcaattccc tcttccgtag caaccgcaac cgcagctgcg
    19981 gcgttcagcg cgttatgacg gcctggcgca ttcagggtga cgcgcatcgg ctctttgtcc
    20041 tggcgcagca gcgtaaagtg cccctgcggg ccaatctgct gataatcttc tacacgcacg
    20101 tcggcatctt cgctgaagcc gtaagtcgtg gtctgacgtc ccacacgcgg taacaattcg
    20161 cggatcaccg gatcatcaac acacatcact gcacgaccgt aaaacggcag gttgtgcaga
    20221 aaattaataa aggtctgttt taaattctca aagtcgccct ggtaggtatc catgtggtcg
    20281 gcttcgatat tggtgacaat cgccaccatc ggttgcagat gcaggaacga tgcatcactc
    20341 tcatctgctt cggcaatcag gtaccgacca tgccccaaac gcgcatgaac gcccgccgct
    20401 tttaccagcc caccgttaac gaaggttggg tcgagtcccg cttctgcgta gatgctggaa
    20461 accatcgcag tggttgtcgt tttgccgtgc gttccggcaa tggcgatgcc atgacgaaaa
    20521 cgcattaact cagccagcat ttcggcacga cggatcaccg gaatacgcgc ttcatgagct
    20581 gcgacaattt ccgggttatc ggcagaaatc gcgctggaaa caacgaccac gctggcatca
    20641 cgtacgtttt ccgggcgatg gttgaaataa atcgtcgccc caagattcat taactgctgc
    20701 gtgaccggat ttggcgctaa atcggaacca ctgatctgat aaccttcatt ggccagaact
    20761 tcggcaatac cgcccatacc ggcaccaccg atgccgacaa aatgtatgtg ccgaacgcga
    20821 cgcatttcgg gcacgatgga acgcagtttt gccaattgtt gtgtattcat tctttacgcc
    20881 attaacttct taaattcata cgatgcaaaa ggcatcgcta caattacgcc cgggcagccc
    20941 ggctcacttc atttgccact cgctcggtgg catccggaat ggatgcagcg cgggcgcgtt
    21001 ctgccatggt taataaggtt tctcgcgacc acccggccag ggtgttggcg acagcatcca
    21061 cgctaagctg tggctgctcg ataattttgg ctgcgcccgc tttttccagc ggtagagcat
    21121 tccagtattg ctggcggtct ttatgttgaa acggcacaaa caacgccggt agtcctgccg
    21181 cagcgatttc actcaccgtt aacgcaccgg agcggcaaac gacgacatcc gcccacgcat
    21241 acgccgccgc catatcatca ataaattccg tcactttatg ctgcgattgc cccgcttcgg
    21301 catacgcctg ttcaacggat tgttgcgaac ctttgccgct ctgatgccag atagtgactg
    21361 aatcacccag tttcacagca acctgcggca ttgtctggtt aagaatgcgt gcgccctggg
    21421 aaccacccac taccaacaca cgaaccggac cttcacgtcc agccaaacgt tgctgcggca
    21481 acggcagcgc cagcacatcg gtacgcaccg ggttacccac cacttccgca ttagggaaag
    21541 cgcctggaaa cgcctgcatc actttggtgg caatcttcgc cagccattta ttggttaagc
    21601 ccgcaatacc gttttgttca tgaagtacaa ccggaatgcc taacgaccac gcggccagac
    21661 cacctgggcc tgatacgtag ccgcccattc cgagcaccac gtcaggtttg tacgctttca
    21721 taatcgcccg cgcctgacgc caggcgttga agattcgcag tggcgcagct atcagtgctt
    21781 ttataccttt tccacgcaga ccagagatac gaatgaaatc aatttcgatg ccatgttttg
    21841 gcactaagtc cgcttccata cggtcggcag tccccagcca gcgaacttgc caaccctgag
    21901 ccattagatg gtgcgcaacc gccagtcccg ggaatacatg tccaccggtt ccgcctgcca
    21961 tcaccattaa tcgctttcct tgaccactca tcgtgaacct cgtacaaacg cctgcgcttt
    22021 ctccagacgc gtttcataat caatacgcaa cagcatcatg atggctgtcg acataatcag
    22081 taagctcgaa ccaccgtaac tgatcagtgg caatgtcaga cctttggtcg gtaacatccc
    22141 cgccgccgcg cctacgttaa ccagcgcctg gaagctaaac cagatgccaa tggaacaggc
    22201 gagaaaaccg gaaaaacgat ggtcaatttc taatgcttta cggccaatcg acatcgcacg
    22261 aaaagcgacg aagaatacca ttaaaagtgc tagtaccaca ccgacataac ctaattcttc
    22321 gccgataatg gcgaaaataa agtcagtgtg cgcttccggc agatactcca gtttttgtac
    22381 cgagttacct aagccttgcc cccaaagttc gccgcgacca aacgccatca gcgattgcgt
    22441 taactgatag ccgctgccaa agggatcttc ccacgggttc cagaatgcgg taacacggcg
    22501 gatacggtac ggttcggcga ggatcagcaa cacaaccgct gaaatgccca taccgataat
    22561 ggcaatgaac tgccacaatt tcgctcccgc caggaataac atcgccagcg tagtcacaaa
    22621 caacaccacc accgtaccaa ggtctggctg tgccagcagt aacactgcca acaccagaat
    22681 cacgcccatc ggtttcagga agccgcgcag gttattacgt acttcgtcgc ctttacgcac
    22741 cagatagttg gcgatatagc aaaacaacga cagttttgtc agctccgcag gctggatacg
    22801 cagcaagccg agatcgatcc aacgcgatgc ccctttaaca gagctaccca ctaccagaac
    22861 gatcatcaac aggatgattg atccgagtag catcgtagcg ctgtaacgtt gccagaactc
    22921 catcggcaga cgcagcgtaa tgatcgccag aatgaatgcc aggatcagat agacgccatc
    22981 acgcttcgcg aagaagaacg gatcgttggt taagcgttgc ccgatgggca ttgacgccga
    23041 ggtcaccatg ataaagccaa tcgccgcgag gccgaaggtc agccacagta aggtgcgatc
    23101 gtacatgatc aggctgtcgg tatctttttc ccgcgagccc atcacccagc cctttagcgc
    23161 cgtggagatc cagaccagaa tactgaatcc tggcaggcgc ggcattttca ggcgagggag
    23221 agataaacgc atcaacctaa ctccttcgcc agacgggcaa actcattgcc tcgttgttca
    23281 aagttcttga actgatcaag gctggcacag gccggggaga gcagaaccat atcgcccggc
    23341 tgaacacgcg gagccagcaa gcgcatcgcc tgttccatgg tttcggtttg ttctgccact
    23401 tccgggcgta gcgccgccag ctgcgcgccg tcccgaccga aacaatacag acgtacgtta
    23461 tcgccattca ggtaacgcgc cagtgggcta aaatctgccg atttaccatc gccgcccagc
    23521 aacaaatgca gcgtgccgtc tacgtgcagg ccattcagtg ccgcttccgt actgccgacg
    23581 ttggtcgctt ttgaatcgtt aatccagcgc acaccgttat gctccagcac aacttcaaag
    23641 cgatgcggca gaccagtgaa tgtggttaac gctttcaggc tgctggcgcg cggcaaacca
    23701 gcggcatctg ccagtgccag agctgccagt gcattggtgt agttatgctg cccggaaagt
    23761 ttcatttctt tcacgttcag cactttctcg cccttcaccc gcagccaggt ttcaccctgc
    23821 tgatgattca ggtgatagtc acccatgttg acgccaaagc tgacgcagcg ttcatccgca
    23881 ccgcgaatcg gcattgttaa ggcatcatca gcattaacca cgcaaacttt cgcgttttcg
    23941 taaatgcgca gttttgctgc acgatactgt tgtaaaccaa acggatagcg atccatatga
    24001 tcttcagtca cgttcagaat ggtcgctgct accgcctgta aactggaggt ggtttcaagc
    24061 tggaagctcg acagctccag cacgtacagt tcgcactcat catccagcag catcaacgca
    24121 ggcaggccaa tattgccacc cacaccaacg ttaacccccg ccgctttcgc catttcaccc
    24181 actagcgtgg tgaccgtgct tttgccgtta gaaccggtga tcgccacaat cggtgcttgt
    24241 gcttcacgac agaacagctc gatatcgcca acgatttcga ttccggcatc agcggcagcg
    24301 cttaaggatg gatgcgccag tgcaataccg ggactggcga caatcagatc ggccgccatc
    24361 agccattcat cattcagacc gcccgtgtgg cgttctacag cttcgggtaa tttatccagg
    24421 ccaggcggtg tcatacgcgt atccataacg cgcggcgtca caccgcgagc gaggaaaaag
    24481 tccacgcaag aaagcccggt gaggcccagg ccgataatga cgacattttt accctggtaa
    24541 tcagccatga ttaacgtacc ttcagcgttg ccagaccaat caggaccagc atcagcgaaa
    24601 taatccagaa acgtacaatg acgcgcggtt ccggccagcc tttcagttca tagtggtgat
    24661 gaatcggtgc catgcggaaa atacgttgtc cgcgcagttt aaaggagccg acctgcagga
    24721 tgacggaaag cgtttctacc acgaacacgc cccccataat caccagcagg aattcctgac
    24781 gcagcagtac ggcgataata cctaacgcac cacctaacgc cagcgaaccg acatcgccca
    24841 taaagacctg cgccggatag gtgttaaacc acaggaagcc cagccctgcc ccgactatcg
    24901 cggtacagac aatgaccagt tccccggcgt gtcgtagata cggtatgtgt aaatagctgg
    24961 caaagttcat gttgccggtc gcccacgcca ccagcgcaaa accaccggcg acaaatacgg
    25021 tcggcataat tgccagaccg tcgagaccat cagtcaggtt taccgcgttg cctgtaccca
    25081 caatgacgaa gtaagccagc agaatgtaga acagccccag ttgcggcatc acatctttaa
    25141 agaacggaac caccagctgc gttgcgggcg tgtctttgcc ggcaaggtac agcgcgaagg
    25201 caacacccag cgcaatgacc gacatccaga aatacttcca acgagcgatc aaccctttgg
    25261 tgtctttacg caccactttg cgatagtcat caacaaagcc aatgatgccg taacctacca
    25321 gcaccaccag tacgcaccag acgtacggat tggatggata agcccacagc agcacggaga
    25381 tcacaatcgc tgtcaggatc ataatcccgc ccatggtcgg cgtaccgcgc ttgctgaagt
    25441 gtgattcagg accgtcgtta cgcactacct gaccaaagga aagtttttgc aaatgagcaa
    25501 tcatacgcgg gcccatccac aatgagatga acagcgcggt cagcaggctg acgatggcgc
    25561 gaaacgtcag ataggaaaag acgttaaagc cggaataata tttgaccaaa tgttcggcca
    25621 gccaaactaa catgtcccat tctcctgtaa agcgcgtact acctcttcca tggcggcact
    25681 acgtgaaccc ttaactaaaa tcgtaattac ctgttgctca gcaatcagtg atttaatacg
    25741 cgcgataagc gctgttttat ctgcaaaatg ttcgccaacg ccgctggcgg tgctgatagc
    25801 atggctttgt ttacccacgc ttaacacgcg gtcaatacca gcagcttttg ccgcctcgcc
    25861 cacctgtaca tggcaggctt cgctttcagc gcccaattcc gccatatcgc ccaccaccag
    25921 cacgcggtag cccggcattt cagccagtac ctggactgct gcggtcattg aaccgacatt
    25981 ggcgttgtag gagtcgtcga gcagcaactg gttttccgcc agttggatgg ggaacagacg
    26041 gcctggaaca gctttcagat ttgccagccc cgctttgata gcatcaagcg ttgcgcccac
    26101 ggacatggag agcgcagcgg ctgccagcgc attcgcaata ttgtgacgcc ctggaagcgg
    26161 cagcagaaca tcgacgctac ctgttggggt ttggagggta aattccgtac cgtgcgaagt
    26221 cacatggatg ttggtggcgg tgaaatcgct gttggcggca ttcggtgaga aacgccacac
    26281 tttgcgtgag ccaattacgc tctgccagtt cagccagtcg ttgttgtcgg cattcataat
    26341 ggcgataccg ttttccggca ggccgctaaa gatttcgcct ttcgcttttg ccacgccagc
    26401 caaagaaccg aaaccttcca gatgcgctgc cgccaggtta ttgaccagcg ccgcttccgg
    26461 gcgagtcaga ctcacggtcc aggcaatttc gccctgatgg ttcgcgccaa gttcaataac
    26521 tgcgtaatcg tattccggcg ttaagcgcag cagcgtcatc ggcacgccga tgtcgttgtt
    26581 gagattgcct gccgtataaa gcgtgttgcc gcactggctt aaaattgccg ccgtcatctc
    26641 tttaacagag gttttgccgg aggagcccgt cagagcaacc acgcgcgccg gaacttgctg
    26701 gcgaacccat gcagccagtt caccaaacgc cagacgcgta tccttgacga ttaactgcgg
    26761 cagatcgata tccagcggac ggctgaccag tagtgcgccc gcgccgccag ctttcgcctg
    26821 gtcggcaaaa tcatgagcat cgaaacgttc gcctttcagg gcaacaaaca ggcagcccgg
    26881 cgtcagtttt cgcgtgtcag tggttacagc atcaagggtg atatctgcac cttgcagttc
    26941 accgttgaga atatcggtaa gttggctaag ggttacgcta atcatgcaat cacccccagc
    27001 agacgcgcca ccgtgacgcg atcggagtag tccagacgct gattgccaac aatctggtaa
    27061 tcctcatggc ctttgcccgc gaccagtacc acatcattct ctttagcctg cataacggcg
    27121 caagtcaccg cttcagcacg gccttccatc actttggcat gtccggcatc taacattccc
    27181 gccagaatat cgttgatgat ggcacgcggt tcttcggtac gcgggttatc gtccgttacc
    27241 accgccacgt cagcgaactc ttcagcaatt gcgcccatca gtggacgttt gcctttatca
    27301 cgatcgccac cacagccaaa gacgcaccac agcttgcccg tacagtgcag gcgcgccgcc
    27361 tgtaaggctt tttccagtgc atctggcgta tgcgcgtaat ccaccaccac cgtcggtttg
    27421 cctggtgcag tgaacacttc catacgtccg caaaccggtt gcagacgcgc ggcggttttc
    27481 agcagctcag ccagtggata gcccagcgcc agcagcgtcg ccagcgcgag cagcaggttg
    27541 ctgacgttaa aagcgcccat cagatggctt tcaatttcgc catcgcccca acttgagcta
    27601 aagcgaatcg tcgcaccgct gtcgtgatag ttcacgtcga tcgcttttaa ccagcgtccg
    27661 tgacaattcg gattaatatg atcttccatt gataccgcaa ccgcgtccgg cagttttgcc
    27721 agccagcggc ggcccacttc atcgtcggcg ttaataatcg cctgaccgca atgatgttca
    27781 gaataaagca gccatttcgc agcttcgtag tgttccatat caccatgata atcaaggtga
    27841 tcgcggctta agttggtaaa gaccgacgcc gcaaatttta atgccgccac acggtgctgt
    27901 accagcccgt gggaggaaac ttccattgcg caaaaagtcg cgccctgatc caccagcccc
    27961 gccagctcat gctgaacatc gactgccgaa ccggtggtat tttctgtcgg gatcactttc
    28021 cccagcagac cgttaccaac ggtgcccatt accgcgctgg tttcgccaag cagttggctc
    28081 cactgcgcca gcagctgggt cgtcgtggtt ttgccgttgg tgcccgttac gcctacaaga
    28141 cgtaaattgt cagagggttc atggtaaaag cggcccgcca gtgcagataa acgctcgttg
    28201 agctggctga gatagatgac cggtacgccg tgcatttcac ggatttcacc gtcggtcgcc
    28261 tcatctttcg cctctgcaat aatggcagcc acaccttgcg ctatcgcctg cgggatatat
    28321 cgacgcccgt ccgcctgatg acctactaca gctacaaaga gatcgcccgc cgcagccaca
    28381 cggctgtcga gtgtcatctc tcgcagtgct cgcgaaggtg cgtctggcac ccacggagca
    28441 agaaggtcgc gcaaattacg atctgccacc tgtcccctcg ccttgattaa tcacaaattc
    28501 atttttatcg cccgttgtca gcgcatccgg ctcgatgttc atggtacgca atacgccgcc
    28561 catgatggca ccaaagaccg gcgcggaaac ggcgccgccg tagtatttac ccgcctgcgg
    28621 atcgttgata acaacaacca gcgcgaagcg cggctgactc gcaggcgcaa cgcctgcggt
    28681 ataagcaata tatttattga tgtagcgacc gtccggccca acctttttcg cggtaccggt
    28741 tttaatggcg atacgatagc ccttaatcgc cgccttcacg ccgccaccac ccggtagcgc
    28801 cacgctttcc atcatatgca ccacggtgcg aacaatggat tccgggaaga ctcgttcacc
    28861 gggaaccggg gggtcaactt tggtaatcga cagtgggcga taaatgccgt agctgccgat
    28921 agttgcgtag actcgcgcta actgtaacgg tgttaccatt agcccgtagc cgaaagagaa
    28981 ggtggccctc tctatgtcag accaccgttg tttttgagga tataagccac tgcgttctcc
    29041 gaccaacccc aaattggtcg cttttcccag tccaaaacgt gagtaagtat ctactaacgc
    29101 tgaggacggc atcgctaacg ccagcttgga aacaccgacg ttactcgact tctgtaatac
    29161 cccggtcagg gttaattcgc tgtagcgtgc cacgtctttg atttcatggc cgttaattcg
    29221 ataaggaacg gtattcagta ccgagttttc ccgcactacg ccacgttgca acgcggtcat
    29281 caccaccatc ggtttaaccg ttgaacccgg ttcaaacacg tcagtgatgg tacggttacg
    29341 catcgcctct ttcggcgtgc cactcagatt gtttgggttg tatgacgggc tgttagccat
    29401 cgccagcact tcaccggtgt tgacatccac cagcacggcg ctaccagatt cagctttgtt
    29461 aaaggccacc gcgttgttca gttcgcgata aaccagcgcc tgcaggcgtt catcaatact
    29521 cagcgccagg ttgtgcgccg cctgactgtc ggtggaagag atatcttcaa ttacgcgacc
    29581 atagcggtct ttacgcacaa tgcgctcacc cggctgcccg gtaagccatt tatcgaaact
    29641 cttctcgacg ccctcaatcc cctgactatc gacgttagta aagccgatga ggtgagcggt
    29701 cacttcgccg gatggataat aacggcgaga ttcttcacgc agatgaatcc ccggcagttt
    29761 cagttttttg atgtactctg ccatgtcagg gtttacctga cgcgccagat aaataaagcg
    29821 ccctttcggg ttggcgttaa tgcgggctga aagctgatcc agcggaatat tgagtgcgtt
    29881 agccagcgcc ttccagcgat caccgacgct gataccgcca gcgtcatgca cttctttcgg
    29941 gtcagcccaa atcgctttta ccggcacgct caccgctaac gggcgaccag aacggtcagt
    30001 aatcatgccg cgggaggtgg aaacttgctg aacgcgaaga gaacgcatgt cgccctcttt
    30061 taccagcata tccggagaga taacttgtaa ccacgctacg cgtccgagca gaaaagccag
    30121 cgccaggaga atacagccgc ataacaacgc aaaacgccaa ctgataaagt tggcatgttc
    30181 ttcctgacgt tttggtttct gcgttttcgc cgctgctttc atgcgtcgcg tttatcctta
    30241 tttttgcact acgatatttt cttgtgacgg atcaacatgc tgcatttgca gcttttccgt
    30301 ggcgatcctt tccacccggc tatggtcgcc gagcgcattc tcttcaagga tcaggttgcg
    30361 ccattcaatg tctaaagcat ctcgctccag caccagttgt tcgcgctgag cggtcagtaa
    30421 acgggtatgg tgcgccgtgg ttaccacggt caccgccgtc aaaataatgc aaatgaacag
    30481 gcagagtggc agcttcccaa atcgcaaaag atcgtcaccg ataacaccag gcaatgcatg
    30541 gcgctcgtgg cttcccatcg atcctttaac tttgcttaga gcttctgtca ctctgctgat
    30601 catgcgttcg tcctctctgc aatacgcaga actgaactac gggcacgagg gttctcagcc
    30661 acctcttctt cgcccggcat taacttgcct agtgctcgca gctgacggcc acccagtttt
    30721 ttgagctgct cttcagtcat cggtaaccct gccggaactt gcggaccgcg gctgttttca
    30781 cgcataaaac gtttcacaat acggtcttcc agcgagtgga agctgatgat cgaaagccgc
    30841 ccacccgggg ccagcacgtt gagcgagctt tttagcgcct gctctatctc ctccagttca
    30901 ctgtttaccc aaatgcgcac cgcctggaag gtccgggtcg cgggatgttt aaacttatct
    30961 ttcaccggcg tagcggcagc cacgacttcc gccagttctt tggtgcgggt catcggctgt
    31021 tcgcggttac gctcgacaat ggcgcgggca atgcgtttgg caaaacgctc ttcaccatag
    31081 gttttcaata cccaggcgat atcggcttct tctgcggttt gtagccattc agcggctgac
    31141 tgcccacggg ttgggtccat acgcatgtcc agcggaccat cgcgcataaa ggaaaagccg
    31201 cgttcagcat catcaagttg cggtgaagag acgccaagat cgaggagaat gccgtcgatc
    31261 ttgccgataa gatcgcgctc ggcaacatat tcgcccagcg cggagaaagg tccgtggatg
    31321 atggagaagc gcggatcatc aatagtcttc gcaacggcga tagcctgcgg gtcgcgatcg
    31381 atcgccagca aacgcccctc ttcgccaagc tgcgagagga tcagacgtga gtgaccaccg
    31441 cgaccaaaag tcccatcaat gtagatgcca tcaggacgga tattgaggcc attaacggct
    31501 tcatccagca gcaccgtagt atgtttatag ttttccatca ttttatagag acaagtcctg
    31561 cagtcgctct gataagtctc cagtagccaa ctgctctgcg tcgatatctt ccttgacctg
    31621 ttgatgccag gttgtttcat cccacagctc aaacttgttg aactgtccaa ccagcatcac
    31681 ttcttttgtc agcccggcat gttgccgcag tactggcgcg attaacaatc gacctgcgcc
    31741 atccatctga cattcgctgg catgacctaa cagtaggcgc tgcacacggc gctcaaccgg
    31801 gttcatgctc gacagacgcg ataatttttg ctcgataatt tcccattcag gcagggggta
    31861 aagcagcagg cacgggtgat gaatgtcaat ggtgcaaacc atttgaccgg cagcgttctc
    31921 aagcagctgc tcccgataac gggtaggcac tgataagcgc cctttgctgt cgagattgac
    31981 taacgttgct ccccggaaca tgccagcctc accccttatt cccactttgc cccacaattt
    32041 cccactgaaa ggagtttacg gagcggagga aaagcttgtc aagccagtca caaggcatac
    32101 cgaggtaaaa aacccgatgt ttacggctaa tatcagcgtt gtttatttta cgtccaacgt
    32161 gcgaagcaaa ttaacgttat gaatagctgt aagaaaaaat ccctcatcgt catcaccgtt
    32221 ttaaaatgac taactatttt ccacagcaaa tataaagtgt cagtttgcga cgcgagcggc
    32281 attttaggac atatcttccc cggttaacag tccctgttgc gtctgtgtgg cgcttgatgc
    32341 cagcgggttt gcgcaaaagc aaagaaaagt gtttgttaaa tcggcaattc atctctgcat
    32401 gtaacaaaat aatgcaaaaa cgcgctatcg atatcactta attattgtga atttcatctg
    32461 gtgaggtaat taacgggcag gtttataagc atttatcaat aatagccagg aatagtctta
    32521 tttactttaa ggatattgat gtccattccc gtactcaacg cgccagagag aaattcacct
    32581 gacgcgtatt tttgttcgcg gcttagctac ggctgagcac gccgcggcga tagagattac
    32641 gtttaatgcg cgttaaacca ggttttggct tacgcggttc gtccaggctt gccaggacaa
    32701 tctccagcac acgctctgcg acatcgcggt gacgttgagc cactgccagc accgggcact
    32761 gtaagaagtc gagcagttcg ttatcgccaa aggtggcaat tgccaggtca gaaggcagtt
    32821 tgccgtcacg acgtagcgtg acatccatca ccccttgcaa caacgcaaac gacgttgtga
    32881 acagtgcctg cggcatcgga tgcgtttcca gccatttttc gaataactgg gcagccgcct
    32941 cccgctcata gctgttggca taaaggaaat gcacttcgcg cggatcatct ttccaggcag
    33001 tacggaaacc ttgttcacgc aggaagctga cagaaagctc cggcagcgcg ccaagataaa
    33061 gtaccgtctc ggcgggaaac ttacgtaact cttccgccag catttcggca tcatcctgat
    33121 cggcaccaac cacgctggtg aaatgttcac gatcgagggc gcggtccagc gcgacaatcg
    33181 ggaacgggtc gttagcccag cgttgataaa aaggatgctc aggtggcaac gacgtcgaaa
    33241 caataatggc atcaacctga cgctgcaaaa gatgctcgat acaacgcatt tcgttgtcgg
    33301 gctgatcttc cgagcaggca atcagcagtt gataacccct ttgccgcgcc tggcgttcaa
    33361 gatagttagc gatgcgggta tagctggtgt tctccagatc ggggatcaca agaccaatag
    33421 aacgtgtgcg tccagcacga agcccagctg ccacggcgtt cgggtggtaa ttgtgctcac
    33481 gcaccacagc catgactttt tcaacggttt tgtcgctcac acggtattgc ttcgctttgc
    33541 cgttaataac atagcttgca gtggtccgcg acactcccgc cagccgagcg atttcatcca
    33601 gtttcacaat tgccccttgc gtaaaatgta aaaaccatag ccattgtact ggtatgggtt
    33661 aaattcatta acatctaagc gcagaaaatt aactgcggca actgctttta ttccaggttc
    33721 ccactgattt cgcaaaaaaa gcccaacgtg acatgttggg ctgcaaattg cgcattgaga
    33781 tcattctcaa cgcattattt tatcgccgcg cgaaagtccg actacaccag agcgagcaac
    33841 ttccacaatt ttcgccacat cgcgaatcga tgctaaaaat gcatcaagct tatcgctggt
    33901 gcctgctaat tgaacggtat aaagcgaggg tgtgacatca ataatttgcc cacggaatat
    33961 ttccgtatta cgtttaactt cgtcacgccc gtagccgctg gcctgaattt tcaccagcat
    34021 gatttcccgc tcaacatgcg cgccctgccc taactcgctc acgcgtaata catcgactag
    34081 cttgtgtaat tgcttttcga tctgctcaag cactttttca tcgcccacgg tctggatggt
    34141 catacgcgat aatgtcggat cgtcagttgg tgcaacggtc aggctttcaa tgttgtagcc
    34201 tcgctgggaa aaaaggccaa tcacgcggga taacgcgcct gattcatttt cgagtaagac
    34261 tgataatatc cggcgcataa tcaggttctc tccgttttgc ttaaccacat ttcatccatt
    34321 ccgcccccgc gaatctgcat cgggtagacg tgctcactgc catcgacggt gacatcaaca
    34381 aacaccaggc gattattacg cacctgttcc agcgcctcgc taagtttgct ttccagttct
    34441 tgcggatgag caatctggat cccgacgtga ccataggctt ccgccagacg aacgaaatcg
    34501 ggtagagatt gcatataaga ttgtgaatga cggccggaat agatcatgtc ctgccactgt
    34561 ttcaccatcc ccagatagcg gttattgaga ttcactacca gtaccggcaa ctcgtattgc
    34621 aacgcggtag acagttcctg gatgttcatc tgaatactgc cgtcgcccgt gacgcaaacc
    34681 acggtttctt ctggcaacgc cattttgacg cccagtgccg caggtaaacc aaaacccatc
    34741 gtgccgaggc cgccagagtt gatccagcga cgcggtttgt cgaatggata ataaagcgca
    34801 gcaaacatct ggtgctgccc gacatcggac gtcacgtaag cgtctccctt cgtcaaccgc
    34861 caaagagtct cgatcaccgc ctgcggttta atcttttcac tgtgagagtc atatttcagg
    34921 cactgacgag cgcgccactg ttcaatctgt tgccaccagt cgcggatctc atccagcggt
    34981 tgatggacgg attcttgcga caggagttca agcatttgtt cgaggacctg gcgagcatcc
    35041 cccacaatcg ggatatcagc agtcacggtt ttagaaatgg aagtaggatc aatatcgata
    35101 tgcagaaccg tggcatttgg gcagtacttc gccagattgt tcgtcgttcg gtcatcaaaa
    35161 cgtaccccga cggcaaaaat cacatccgcg ttatgcatcg tcatattagc ttcgtaggtg
    35221 ccgtgcattc ccagcatgcc cagtgcctga cgatgcgttg ccggaaacgc ccccagcccc
    35281 attaatgagg aaacaacggg caggttcaac gcctccaccg tttcttttag ctgctgatga
    35341 cagcccgccg tgattgcccc accgcctaca tagacaaccg gtttttttgc cgccaccagc
    35401 gtttgcagag cacgcttaat ttgcccttta tgcccggagg tcgtgggatt gtaagatcgc
    35461 atactgaccg actccggcca gacgtagggt aatttgttcg ccggattaag aatatctttg
    35521 ggtaaatcaa cgaccacggg cccagggcga ccacttgccg ccagccagaa agcctttttc
    35581 agcacctgcg gaatgtcttc cgtttgctta accagaaaac tgtgtttaac caccgggcga
    35641 gaaatcccca ccatgtcgca ctcctgaaag gcatcgtagc ctatcaacga ggtcgctacc
    35701 tgcccggaaa ggacaactaa tggaatggaa tccatataag cggtggcaat gccagtaatc
    35761 gcattggtcg cccctggacc cgacgttacc agcacgacgc cgacttcccc ggtcgcgcgc
    35821 gccagaccat cggccatatg caccgccgcc tgctcatgac gaactaaaac atgatcgata
    35881 ccacccacgg tatgcaatgc atcgtaaata tcaaggaccg cgcctccggg ataaccgaat
    35941 acttgtttaa cgccctgatc gataagcgat cggacgacca tctcggctcc agacaacatc
    36001 tccatggcct gcctcactgt ttgacggaaa aaaatgtgta aaacacatta accgtccgat
    36061 cttgcttaag caattggcag ccagagacat aaacacgggg taataagaat aaacaggagg
    36121 aaaggtgaaa agaaacgata aaatcctcca ttacagagga ttaaattgat aataaatcgg
    36181 ctgaatccca caacttacag caatttctct atttttttaa ttaatcctaa aatagagagc
    36241 acacacattc agaataaaat tctacattca ataaaaaatg cagaataaac cagacattga
    36301 tgtctgacct attctgcatt cagttagcgt ttgcaaattg agactaattg ctcttccatc
    36361 cactgatggc ctttatcgcg cccggcagct tcatgccagg agagataaca ggttctgctg
    36421 ttttgtttta acggcagcgg taatacctgt aattctaagg attcagcgaa ctcttccgcc
    36481 agccaacgcg gcgcaatagc gaccagatgc gtttgcgaca ccacgctaag tacgctcatc
    36541 attgccatgc cctgatacgc gatactggct tgcttatcta ccgtgtcata ccacggttga
    36601 ctaaatgacg cgaaacgatc gagcgaaacc gccgcatgtt gttcgttata aacatcatgt
    36661 ttcagtaacg ggcctttaat tgttggatga tttttgctgg ctaccagcac catttcatct
    36721 ttaaataatg gcacactggt aaattcagga cgatggaagt cttcataact aatcacaaac
    36781 tccgtttcct gataacgcag ctgatgttca gtgttctgat ttaatgaaga cttgaacata
    36841 acatgtatat ttggcgcaat ctgctcaatg tgattataaa tctgcgaggt cagaatgctg
    36901 tctaacgggc tgcagacaca aagatgaaat acacgttcac tactggcagg ctcaaagcct
    36961 gaacctggta attcattttg tactaattgc aatgcctgac gaactgaacc aaaaagttga
    37021 aatgcgcgag cagtcggttg aataccacgg ccataacgaa caaaaagctc gtcattaaac
    37081 atcaccttca ggcgtgcaac agcgttactg accgcaggtt gcgacattcc cagaacatga
    37141 gcggcacggg taatgttttg ctcctgcatc acggcatcga aaacggttaa taagttgaga
    37201 tcgaccatgc gtagctgtgg cttgcttaac tccgccgtct ctggatgatc tgtttgtacc
    37261 tctggcatac ttaactccac tgtcacactt aactcccttt cccttattgg aatgcagaga
    37321 aataatcctc gagaatatga tttatcatgc atataaaata agaaaaag
//
LOCUS       DACBVI010000028        37176 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.028, whole genome shotgun sequence.
ACCESSION   DACBVI010000028 DACBVI010000000
VERSION     DACBVI010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.028"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(97..1761)
                     /gene="glnS"
                     /locus_tag="IU325_07080"
     CDS             complement(97..1761)
                     /gene="glnS"
                     /locus_tag="IU325_07080"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HAP5808804.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1964..3910)
                     /gene="nagE"
                     /locus_tag="IU325_07085"
     CDS             complement(1964..3910)
                     /gene="nagE"
                     /locus_tag="IU325_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HAP5808805.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVVDSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAETAPATAAPVAKPQAIPNAVSVAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNENARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4243..5043
                     /gene="nagB"
                     /locus_tag="IU325_07090"
     CDS             4243..5043
                     /gene="nagB"
                     /locus_tag="IU325_07090"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP5808806.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     YVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5103..6251
                     /gene="nagA"
                     /locus_tag="IU325_07095"
     CDS             5103..6251
                     /gene="nagA"
                     /locus_tag="IU325_07095"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP5808807.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVHVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANSGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6260..7480
                     /gene="nagC"
                     /locus_tag="IU325_07100"
                     /gene_synonym="nagR"
     CDS             6260..7480
                     /gene="nagC"
                     /locus_tag="IU325_07100"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HAP5808808.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7528..8280
                     /gene="nagD"
                     /locus_tag="IU325_07105"
     CDS             7528..8280
                     /gene="nagD"
                     /locus_tag="IU325_07105"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HAP5808809.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8537..10201
                     /locus_tag="IU325_07110"
     CDS             8537..10201
                     /locus_tag="IU325_07110"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HAP5808810.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10581..10657
                     /locus_tag="IU325_07115"
     tRNA            10581..10657
                     /locus_tag="IU325_07115"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10615..10617,aa:Met,seq:cat)
     gene            10666..10750
                     /locus_tag="IU325_07120"
     tRNA            10666..10750
                     /locus_tag="IU325_07120"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10700..10702,aa:Leu,seq:tag)
     gene            10774..10848
                     /locus_tag="IU325_07125"
     tRNA            10774..10848
                     /locus_tag="IU325_07125"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10806..10808,aa:Gln,seq:ttg)
     gene            10883..10957
                     /locus_tag="IU325_07130"
     tRNA            10883..10957
                     /locus_tag="IU325_07130"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10915..10917,aa:Gln,seq:ttg)
     gene            10973..11049
                     /locus_tag="IU325_07135"
     tRNA            10973..11049
                     /locus_tag="IU325_07135"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11007..11009,aa:Met,seq:cat)
     gene            11098..11172
                     /locus_tag="IU325_07140"
     tRNA            11098..11172
                     /locus_tag="IU325_07140"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11130..11132,aa:Gln,seq:ctg)
     gene            11210..11284
                     /locus_tag="IU325_07145"
     tRNA            11210..11284
                     /locus_tag="IU325_07145"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11242..11244,aa:Gln,seq:ctg)
     gene            complement(11438..12613)
                     /gene="ubiF"
                     /locus_tag="IU325_07150"
     CDS             complement(11438..12613)
                     /gene="ubiF"
                     /locus_tag="IU325_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HAP5808811.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAQSATFVV
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PVLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRHNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPILKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGMLKRQALKYALGL"
     gene            12759..14183
                     /gene="miaB"
                     /locus_tag="IU325_07155"
     CDS             12759..14183
                     /gene="miaB"
                     /locus_tag="IU325_07155"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HAP5808812.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14297..15376
                     /locus_tag="IU325_07160"
     CDS             14297..15376
                     /locus_tag="IU325_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP5808813.1"
                     /translation="MFSFRRHTDKRNGLNIDTREITLEPADNARLLSLCGPFDDNIKQ
                     LERRLGIEINRRDNHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAI
                     KEARVLEQSAESVPEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTG
                     KTYLAVAAAVDALERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFE
                     MLGFEKVEKLIERNVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNS
                     KAVITGDVTQIDLPRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYE
                     AWEEAEQKRKAALAAERKREEQEQK"
     gene            15373..15840
                     /gene="ybeY"
                     /locus_tag="IU325_07165"
     CDS             15373..15840
                     /gene="ybeY"
                     /locus_tag="IU325_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP5808814.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            15930..16808
                     /gene="corC"
                     /locus_tag="IU325_07170"
     CDS             15930..16808
                     /gene="corC"
                     /locus_tag="IU325_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HAP5808815.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16833..18371
                     /gene="lnt"
                     /locus_tag="IU325_07175"
     CDS             16833..18371
                     /gene="lnt"
                     /locus_tag="IU325_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HAP5808816.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNVFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLAVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            18768..19676
                     /gene="gltI"
                     /locus_tag="IU325_07180"
     CDS             18768..19676
                     /gene="gltI"
                     /locus_tag="IU325_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HAP5808817.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            19846..20586
                     /gene="gltJ"
                     /locus_tag="IU325_07185"
     CDS             19846..20586
                     /gene="gltJ"
                     /locus_tag="IU325_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HAP5808818.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            20586..21260
                     /gene="gltK"
                     /locus_tag="IU325_07190"
     CDS             20586..21260
                     /gene="gltK"
                     /locus_tag="IU325_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HAP5808819.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            21260..21985
                     /gene="gltL"
                     /locus_tag="IU325_07195"
     CDS             21260..21985
                     /gene="gltL"
                     /locus_tag="IU325_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HAP5808820.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            22103..23038
                     /gene="rihA"
                     /locus_tag="IU325_07200"
     CDS             22103..23038
                     /gene="rihA"
                     /locus_tag="IU325_07200"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HAP5808821.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLNPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGLVDLLADRLKFYA"
     gene            complement(23083..23565)
                     /locus_tag="IU325_07205"
     CDS             complement(23083..23565)
                     /locus_tag="IU325_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HAP5808822.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            23800..26382
                     /gene="leuS"
                     /locus_tag="IU325_07210"
     CDS             23800..26382
                     /gene="leuS"
                     /locus_tag="IU325_07210"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP5808823.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            26397..26978
                     /gene="lptE"
                     /locus_tag="IU325_07215"
     CDS             26397..26978
                     /gene="lptE"
                     /locus_tag="IU325_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HAP5808824.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            26978..28009
                     /gene="holA"
                     /locus_tag="IU325_07220"
     CDS             26978..28009
                     /gene="holA"
                     /locus_tag="IU325_07220"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP5808825.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAIRQVAAV
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLSENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            28011..28652
                     /gene="nadD"
                     /locus_tag="IU325_07225"
     CDS             28011..28652
                     /gene="nadD"
                     /locus_tag="IU325_07225"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP5808826.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            28676..29287
                     /locus_tag="IU325_07230"
     CDS             28676..29287
                     /locus_tag="IU325_07230"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HAP5808827.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARRVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            29547..29864
                     /gene="rsfS"
                     /locus_tag="IU325_07235"
                     /gene_synonym="rsfA"
     CDS             29547..29864
                     /gene="rsfS"
                     /locus_tag="IU325_07235"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP5808828.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            29868..30335
                     /gene="rlmH"
                     /locus_tag="IU325_07240"
     CDS             29868..30335
                     /gene="rlmH"
                     /locus_tag="IU325_07240"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP5808829.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            30366..32267
                     /locus_tag="IU325_07245"
     CDS             30366..32267
                     /locus_tag="IU325_07245"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="penicillin-binding protein 2; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HAP5808830.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            32270..33382
                     /gene="mrdB"
                     /locus_tag="IU325_07250"
                     /gene_synonym="rodA"
     CDS             32270..33382
                     /gene="mrdB"
                     /locus_tag="IU325_07250"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HAP5808831.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            33393..34481
                     /gene="rlpA"
                     /locus_tag="IU325_07255"
     CDS             33393..34481
                     /gene="rlpA"
                     /locus_tag="IU325_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HAP5808832.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTNSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPATTPATTPA
                     MVTPQATSQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            34621..35832
                     /gene="dacA"
                     /locus_tag="IU325_07260"
     CDS             34621..35832
                     /gene="dacA"
                     /locus_tag="IU325_07260"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HAP5808833.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            35942..36205
                     /locus_tag="IU325_07265"
     CDS             35942..36205
                     /locus_tag="IU325_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HAP5808834.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            36306..36947
                     /gene="lipB"
                     /locus_tag="IU325_07270"
     CDS             36306..36947
                     /gene="lipB"
                     /locus_tag="IU325_07270"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HAP5808835.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDESTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         9054 a         9659 c         9633 g         8830 t
ORIGIN      
        1 gaaaaaaaac gccgcggagc gcggcgtttg ggataatttg catatttctt gccggatgcg
       61 gcgtaaacgc ctcacccggc ctggtagaac ttaaaattac tcgcctactt tcgcccaggt
      121 atcacgcagc ccaacggtgc ggttaaatac cggtttttcc gccgtagaat ggcggctgtc
      181 gaggcagaaa taaccttcac gctcaaactg gaatgcttta cccgctaccg cgtctttcag
      241 agacggttca gcaaagccct gtttgatcac cagcgattcc gggttaatca ccgacaggaa
      301 atcatccgca gcacctgggt tcggcacgct gaacagacga tcgtacaggc ggatttcaac
      361 cggcagcgca tgtgccgcgc tcacccagtg gataacacct ttcactttac gaccatctgc
      421 cggatctttg cttaaggtgt cggcgtcata agtacagaag atggtggtga tattgccttc
      481 ggcatctttc tccacgcgtt ctgccttaat gacgtaagca ttacgcagac gcacttcttt
      541 acccagcacc agacgcttgt actgcttgtt agcttcttcg cggaaatcgg cgcggtcgat
      601 ccaaatctca ccgctaaacg gcacctgacg gctccccatt tccggtttgt tcggatggtt
      661 cggcatggta accatttcgc cttcgccctg gtagttttcg ataaccagtt tcaccggatc
      721 gataaccgcc attgcgcgcg gggcgttttc gttgagatct tcacggatgc aggattccag
      781 cgacgccatt tcaatggtgt tgtcctgctt ggtcacgccg atgcgtttgc agaactcacg
      841 aatagaagcc gcagtgtaac cacgacgacg cagaccggaa atggtcggca tacgcgggtc
      901 atcccagcct tcaacgtgct tgtcggtcac cagcaggttc agcttacgct tggacatcac
      961 agtgtattcc agattcaggc gcgagaattc gtactggcgc gggtgaacag gaatggtgat
     1021 gttgtccagc acccagtcgt acaggcgacg gttgtcctgg aactcaagcg tacacagaga
     1081 gtgcgtaata ccttccagcg catcgctgat gcaatgggtg aagtcgtaca tcgggtagat
     1141 gcaccacttg ttgccagtct ggtggtgttc agcaaattta atgcggtaca gcaccggatc
     1201 gcgcattacg ataaatggcg acgccatgtc gattttcgca cgcaggcagg ctttaccttc
     1261 ttcaaaacca ccggtacgca ttttttcgaa cagcgccagg ttctcttcaa cgctgcggtc
     1321 gcggtacggg ctgtttttac ccggctgcgt cagggtgccg cggtattcgc ggatctgttc
     1381 cggtgtcagt tcatcaacgt acgccaggcc tttattgatc agttcgatcg cataggcgtg
     1441 gagctgatca aaataatcgg aggagtaacg gacgttacca gaccagtgaa aacctaacca
     1501 ctctacgtcg tttttgatcg actcaacata ctcgatatct tcttttaccg ggttagtgtc
     1561 gtcgaaacgc aggttgcact ggcctttata gtcctgggcg atcccgaagt tcaggcagat
     1621 agatttcgca tggccaatat gcagatagcc attcggctcc ggcgggaaac gggtgtgtac
     1681 tgtggtgtgc ttaccactgg ccagatcttc atcgatgatc tgacgaataa agttagtcgg
     1741 gcgggcttct gcctcactca tcgtggattc ctcaaagcgt aaacaacgta taacggcgta
     1801 tgatcttata agcgggacag gctgacaact gttagttagt gaaaatcgaa aatctcactg
     1861 agattatggg gatgattgat gaaaaaaaaa gcggcagaga agatctccac cgcttgtcat
     1921 tgttggatgc gacgctgccg tgtcgcatca ggtataaagc agattacttt ttgatttcat
     1981 acagcggtgt ttgacccgcc acgacatggc cctgagcttt aatgatcaag ccgctgaaat
     2041 cgtcgatatt gctgcaaacc accgggctaa tcatcgagcg ggcgttttcg ttcagataat
     2101 ccagatccat ttccagaatc ggttgccctg cgcttacttg cgcgccctct tccaccagac
     2161 gtttaaagcc tttaccttcc agcgctacgg tgtcgatacc catatggacg acgatctccg
     2221 cgcctttttc ggtttccagg cagaacgcgt ggttggtgtt gaagattttc acgattgtcc
     2281 ctgcggctgg tgatacgacg attttatctg tcggtttcac cgccacaccg tcacccaccg
     2341 ctttgctggc gaatgcttcg tcaggaacct gatccagtgc cacaacatca ccggtaatcg
     2401 gcgataccag ctccgcgaca gacaccgcgt ttggtatagc ctgcggtttt gctacaggcg
     2461 cggcagttgc aggcgcagtt tcagctgacg cagcggctac cggaccacgg gcaacaactt
     2521 tcttcattgc atcgccaatg gattccgctt tcgcgccaac aatcacctga atagtctgtt
     2581 tgttcagttt cactacccca gaagcaccca gacgtttaca catcgcatcg ttgacgcgag
     2641 ccgagtcaac cacggtcagg cgcagacgag taatacaggc gtcaattgct ttcaggttgt
     2701 cagtgccgcc aaccgcagca atatagttgg tcgccagttg attgaggcct tcttcagtgt
     2761 tgctgttggc ttcttcagta acgatctcat cttctttatc ttcacgaccc ggcgttttca
     2821 ggttgaacat gcggataacc aaactgaaca ccacgaagta gatagcgaag aagacaacac
     2881 ccatcaccag cagcatccag acgttctggc tggcggccgg caggttatac atcaacgcgt
     2941 agtcgatagc ccccgcagag aaggagaagc ccgcgtggat acccagcagc gttgccacaa
     3001 acaggctgat accggtcagc agtgcgtgca ggaggtacag cagcggcgca aggaacatga
     3061 acaggaattc cagcggctca gtcacaccgg tcaggaacgc agtaacagca acagaaagca
     3121 gcataccgcc aaccatcgga cgacgctctt tcggtgctgc aaagtacatc gccagcgccg
     3181 cacccggcag accgaacatc atgatcggga agaagccgga catgaacatc cccgcggtgc
     3241 cgtcaccggc atagaagcgg ttgatgtcac cgtggaaaac cgtacccgcc gcgttggtga
     3301 attcaccaat ctggaaccag gcgatggtgt tcagtacctg atgcagaccg gttgggatca
     3361 gcagacggtt gatgaaacca aagataccgg aacccagcgc gcccgcagaa acgatccact
     3421 cgccgcctgc atggatagcg tgctgtaccg gcggccagac gtaaccaaaa atggccgcca
     3481 gcaccaggca gaagaagccg gtggcaatcg gcacaaagcg tttgccgccg aagaaactca
     3541 ggaagtccgg cagtttaata tcggaccaac ggttataagc tgcgccacca accagaccgg
     3601 taatgatacc cgccagtaca cccatgttaa tttccgggtt gatggtcacc atcgctttgg
     3661 ttaacacaaa gtaacctacc gcacccgcca gcgccgccgc acctgcgctg tctttcgacc
     3721 agctggatgc cacaccgatg gcgaagatta atgcgaggtt atcaaaaatc gcaccgcccg
     3781 cctgggcaat aaacgcaacg ttaagtaaat ctggctgacc gaatcgcagc aacagcgccg
     3841 ccaccggcag caccgcgata gggagctgta acgccctacc gagtcgctgg aaaaaaccta
     3901 aaatattcat cttattcccc ctacgagaac cctatttggc tcgtttaaag ccgtattttt
     3961 attttgctgc aaattgtact gccgatgttc tgtaatcaga ttgttagatc atctgctaca
     4021 gagtgtgtga aaatttaatt cgtatcgcaa attaaacgcg tgtcttttgt gagttttgtc
     4081 accaaatatc gttatcatca ctccctttta ctggctaaac cagaaaactt attttatcat
     4141 tcaaaaaatc aggtcggatt gacgcctgtc tgcgcaaatc caggttacgc ttaaagatgc
     4201 ctaatccgcc aacggcttac attttactta ttgaggtgaa taatgagact gatccccctg
     4261 actaccgctg aacaggtcgg caaatgggct gctcgccata tcgtcaatcg tatcaatgcg
     4321 ttcaaaccga ctgccgatcg tccgtttgtt ctgggcctgc cgactggcgg cacgccgatg
     4381 accacctata aagcgttagt cgaaatgcat aaagcaggcc aggtcagctt taagcacgtt
     4441 gtcaccttca acatggacga atatgtcggt ctgccgaaag agcatccgga aagctactac
     4501 agctttatgc accgtaattt cttcgatcac gttgatattc cagcagaaaa catcaacctt
     4561 ctcaacggca acgccccgga tatcgacgcc gagtgccgcc agtatgaaga aaaaatccgt
     4621 tcttacggaa aaattcatct gtttatgggc ggtgtaggta acgacggcca tattgcattt
     4681 aacgaaccgg cgtcttctct ggcttctcgt actcgtatca aaacactgac tcatgatact
     4741 cgcgtcgcga actctcgttt ctttgataac gatgtcaatc aggtgccaaa atatgccctg
     4801 actgtcggtg ttggtacatt gctggatgcc gaagaagtga tgattctggt gctgggtagc
     4861 cagaaagcac tggcactgca ggccgccgtt gaaggttacg taaaccacat gtggaccatc
     4921 agctgtctgc aactgcatcc aaaagcgatc atggtgtgcg atgaaccttc caccatggag
     4981 ctgaaagtta agactttaag atatttcaat gaattagaag cagaaaatat caaaggtctg
     5041 taatagttat ccctgccctg acaccttgct cagggcaata ttttttaaaa tcgggggtca
     5101 gaatgtatgc attaacccag ggccggatct ttaccggcca cgaatttctt gatgaccacg
     5161 cggttgttat cgctgatggc ctgattaaaa gcgtctgtcc ggtagcggaa ctgccgccag
     5221 agatcgaaca acgttcactg aacggggcca ttctctcccc cggttttatc gatgtgcagt
     5281 taaacggctg cggcggcgtg cagtttaacg atactgctga agcggtcagc gtagaaacgc
     5341 tggaaatcat gcagaaagcc aatgagaaat caggctgtac taactatttg ccgacgctta
     5401 tcaccaccag cgacgagctg atgaaacagg gcgtgcacgt tatgcgcgag tacctggcaa
     5461 aacatccgaa tcaggcgtta ggtctgcatc tggaaggtcc gtggctgaat ctggtaaaaa
     5521 aaggcaccca taatccgaat tttgtgcgta agcctgatgc cgcgctggtc gatttcctgt
     5581 gtgaaaacgc cgacgtcatt accaaagtga cgctggcacc ggaaatggtt cctgcggaag
     5641 tgatcagtaa actggcaaat tccgggattg tggtttctgc cggtcactcc aacgcgacgt
     5701 tgaaagaagc aaaagccggt ttccgcgcgg ggattacctt tgccactcat ctgtacaacg
     5761 cgatgccgta tattaccggt cgtgaaccgg gcctggcggg cgcgattctc gacgaagctg
     5821 acatttattg cggtattatc gctgatggcc tgcatgttga ttacgccaac attcgcaacg
     5881 ctaaacgtct gaaaggcgac aaactgtgtc tggttactga cgccaccgca ccagcaggtg
     5941 ccaacattga acagttcatt tttgcgggta aaacaatata ctaccgtaac ggactttgtg
     6001 tggatgagaa cggtacgtta agcggttcat ccttaaccat gattgaaggc gtgcgtaatc
     6061 tggtcgaaca ttgtggtatc gcactggatg aagtgctgcg tatggcgacg ctctatccgg
     6121 cgcgtgcgat tggcgttgag aaacgtctcg gtacactcgc cgcaggtaaa gtagccaacc
     6181 tgactgcatt cacacctgat tttaaaatca ccaagaccat cgttaacggt aacgaggtcg
     6241 taactcaata agagaaagta tgacaccagg cggacaagct cagataggta atgttgatct
     6301 cgtaaaacag cttaacagcg cggcggttta tcgcttgatt gaccagtacg ggccaatctc
     6361 gcggattcag attgccgagc aaagccagct tgcccccgcc agcgtaacca aaattacgcg
     6421 tcagcttatc gaacgcgggc tgattaaaga agttgatcag caggcctcca ccgggggccg
     6481 ccgcgctatc tctatcgtca ccgaaacccg caatttccac gcaatcggcg tacggcttgg
     6541 tcgtcacgac gccaccatca ctctgtttga tctcagtagc aaagtgctgg cagaagaaca
     6601 ttacccgctg ccggaacgta cccagcagac gctggaacat gccctgctga atgccattgc
     6661 tcagtttatt gatagctacc agcgcaaact gcgcgagctg atcgccattt ccgtgatcct
     6721 gccaggactt gttgacccgg acagcggcaa aattcattac atgccgcata ttcaggtaga
     6781 aaactggggg ctggtagaag ctctggaaga acgttttaaa gtgacctgtt tcgtcggtca
     6841 cgatatccgt agtctggcgc tggcggagca ctacttcggt gcaagtcagg attgcgaaga
     6901 ctccattctg gtgcgtgtac atcgcggaac cggggccggg attatctcta acgggcgcat
     6961 ttttattggc cgcaacggta acgtcggtga aattggccat attcaggtcg aaccgctggg
     7021 tgaacgctgc cactgcggca actttggctg cctggaaact atcgccgcca acgctgccat
     7081 tgaacaacgg gtgttgaatc tgttaaagca gggctaccag agccgtgtgc cactggacga
     7141 ctgcaccatc aaaactatct gcaaagccgc gaacaaaggc gatagcctgg cgtcggaagt
     7201 gattgagtat gtcggtcgtc atctgggcaa aaccatcgcc attgctatca acttatttaa
     7261 tccgcaaaaa attgttattg ccggtgagat caccgaagcc gataaagtgc tgctccctgc
     7321 tattgaaagc tgcattaata cccaggcgct gaaggcgttt cgtactaatc tgccggtggt
     7381 acgttctgag ctggatcacc gctcggcaat cggcgctttt gcgctggtaa aacgcgccat
     7441 gctcaacggt attttgctcc agcatttgct ggaaaattaa tgtgctttta tagtggcgct
     7501 tattgttgtc aatattctgg gtagtccatg accattaaaa atgtaatttg cgatatcgac
     7561 ggcgtgctga tgcacgataa cgtcgccgta ccgggtgcag cggaattttt gcacgggatt
     7621 atggataaag gcctgccgct ggtgttgctg accaactatc cttcgcagac cgggcaagat
     7681 ctggcgaacc gctttgccac tgccggtgtc gatgtaccgg atagcgtgtt ttatacctcc
     7741 gcgatggcaa ccgccgattt cctgcgtcgc caggaaggca agaaagcata tgtggtgggc
     7801 gaaggcgcac tgattcatga actgtacaaa gccggtttca ctattaccga tgtgaaccct
     7861 gattttgtga ttgttggcga aacgcgttcc tacaactggg acatgatgca taaagcagcc
     7921 tatttcgtcg ctaacggtgc acgctttatc gccaccaacc cggacaccca cgggcgcggt
     7981 ttttatcccg cgtgtggcgc gttgtgtgca ggcatcgaaa aaatctccgg gcgtaaaccg
     8041 ttctatgttg gtaaaccgag tccgtggatc atccgcgcag cattaaacaa aatgcaggcg
     8101 cattcggaag aaacagtgat tgtcggcgat aacctgcgta ccgatattct ggcgggcttc
     8161 caggccggtc tggagacgat tctggtactc tctggcgttt cgtcgctcga cgatatcgac
     8221 agtatgccat tccgccccag ctggatttac ccatcggtcg ctgaaatcga cgttatctga
     8281 atagcaacca cgcctcaggg cgtggtttct tatttttacc catcattaaa attcacccat
     8341 tcaataaaac ccgccaaaaa ttatcgattc atcaataaat acacctccac accccaccct
     8401 ttttcattaa tcagcaatca ccattacgtt ttttatttac cccctgccaa atcgcttgcg
     8461 tacagtgctg ctttgcggca tttttttaaa caagcaaaca caacaagcaa caaataccag
     8521 gttaacggag aaggttatgt gttcaatttt tggcgtattc gatatcaaaa cagacgcagt
     8581 tgagctgcgt aagaaagcac tcgaactgtc acgcctgatg cgtcatcgtg gcccggactg
     8641 gtccggtatt tatgccagcg ataacgccat tctcgcccac gaacgtctgt caattgttga
     8701 cgttaacgcg ggggcgcaac ctctctacaa ccaacaaaaa actcatgtgc tggcggtaaa
     8761 cggtgaaatc tacaaccacc aggcactgcg cgccgaatat ggcgatcgtt atcagttcca
     8821 gaccggatct gactgcgaag tgatcctcgc gctgtatcag gaaaaagggc cggaatttct
     8881 cgacgacttg cagggcatgt ttgcctttgc cctgtacgac agcgaaaaag atgcatatct
     8941 gattggtcgc gaccatctgg ggatcatccc actgtatatg ggctatgacg aacacggtca
     9001 gctgtatgtg gcctcagaaa tgaaagccct ggtgccagtt tgccgcacga ttaaagagtt
     9061 cccggcgggg agctatttgt ggagccagga cggcgaaatc cgttcttatt atcaccgcga
     9121 ctggttcgac tttgatgcag tgaaagataa cgtgaccgac aaaaacgagc tgcgtcaggc
     9181 actggaagat tccgttaaaa gccatctgat gtctgacgtt ccttacggtg tgctgctatc
     9241 tggtggcctg gattcctcaa tcatttccgc gatcaccaag aaatacgccg cccgtcgcgt
     9301 ggaagatcag gaacgctctg aagcctggtg gccgcaatta cactcctttg ctgtaggtct
     9361 gccgggttca ccggatctga aagcagccca ggaagtggca aaccatctgg gcacggtgca
     9421 tcacgaaatt cacttcactg tacaggaagg tctggacgcc atccgcgacg tgatttacca
     9481 catcgaaacc tatgatgtga ccactattcg cgcttcaaca ccgatgtatt taatgtcgcg
     9541 gaagatcaag gcgatgggca ttaaaatggt gctgtccggt gaaggttctg atgaagtgtt
     9601 tggcggttat ctttacttcc acaaagcacc gaatgccaaa gaactgcatg aagagacggt
     9661 gcgtaaactg ctggccctgc atatgtatga ctgcgcgcgt gccaacaaag cgatgtcagc
     9721 ctggggcgtg gaagcacgcg ttccgttcct cgacaaaaaa ttcctcgacg tggcgatgcg
     9781 cattaaccca caggataaaa tgtgcggtaa cggcaaaatg gaaaaacaca tcctgcgtga
     9841 atgttttgag tcgtacttgc ccgcaagtgt ggcctggcgg cagaaagagc agttctccga
     9901 tggcgtcggt tacagttgga tcgacaccct gaaagaagta gcggcacagc aggtttctga
     9961 tcaacagctg gaaactgccc gcttccgctt cccgtacaac acgccgacgt ccaaagaagc
    10021 gtatctgtac cgcgagatct ttgaagaact gttcccgctt ccgagcgccg ctgagtgcgt
    10081 gccgggcggc ccttccgtcg cgtgttcttc cgctaaagcg atcgagtggg atgaggcgtt
    10141 caagaaaatg gacgatccgt ctggtcgtgc ggttggtgtt caccagtcgg cgtataagta
    10201 agaaatgcgg tactgccccg gagaaatccg gggctgtttt agttattgag ctgcttgttg
    10261 attctgatat gcagtaagga aatgttccgg gtttgccaca tcactgtttg acaactcatc
    10321 actgtaggcc agtacttcat agattaattt cccaccacta ttgttgaatt ccgaatttca
    10381 tcctgtatga caaaagaatg gctgtagggt ggggaaaaaa cacaaagtaa acaataattg
    10441 acgaatatag cgccacgctg ttcgcaacct aaccaaacag tcactttcga gcaattttcc
    10501 ttgaaaaaga ggttgacgct gcaaggctct atacgcataa tgcgccccgc aacgccgata
    10561 aggtatcgcg aaaaaaagat ggctacgtag ctcagttggt tagagcacat cactcataat
    10621 gatggggtca caggttcgaa tcccgtcgta gccaccatct tttttgcggg agtggcgaaa
    10681 ttggtagacg caccagattt aggttctggc gccgcaaggt gtgcgagttc aagtctcgcc
    10741 tcccgcacca ttcaccagaa agcgttgtac ggatggggta tcgccaagcg gtaaggcacc
    10801 ggtttttgat accggcattc cctggttcga atccaggtac cccagccatc ttcttcgagt
    10861 aagcggttca ccgcccggtt attggggtat cgccaagcgg taaggcaccg gtttttgata
    10921 ccggcattcc ctggttcgaa tccaggtacc ccagccatcg aagaaacaat ctggctacgt
    10981 agctcagttg gttagagcac atcactcata atgatggggt cacaggttcg aatcccgtcg
    11041 tagccaccaa attctgaatg tgtcgaatat gttcggcaaa ttcaaaaacc aatttgttgg
    11101 ggtatcgcca agcggtaagg caccggattc tgattccggc attccgaggt tcgaatcctc
    11161 gtaccccagc caatttattc aagacgctta ccttgtaagt gcacccagtt ggggtatcgc
    11221 caagcggtaa ggcaccggat tctgattccg gcattccgag gttcgaatcc tcgtacccca
    11281 gccacattaa aaaagctcgc ttcggcgagc tttttgcttt tctgcgtata ttcaatgtcg
    11341 aatgcgatgt tgacacgtct tatccttcaa agtcggatgc gacgctgccg cgtcttatcc
    11401 gacctacggt tggcacgcat ccggcaatgt tgtaaggcta caaccctaac gcatatttca
    11461 gcgcctgacg tttcaacatg ccagcacgct ccgccgccat caaaccaaga ttacgcacaa
    11521 aacgcagtgg tggcagatta ttgctgaatc cggcatagaa cagatccata ccgctctgca
    11581 taatgaagtt atccgccatg cgccgcatct ggtaacgctt gaggatcgga taactggccc
    11641 acgcttcgcc atagctgcga gcgttgacca gaacgtcaat cagggcatcg acatcacgat
    11701 aaccaagatt caccccctgc cccgccagcg gatggatggt atgcgcggca tcgcccacca
    11761 gcgcaagccc cggctgcaca tactgcaacg catggcggcg cgtcagcgga aaagcgccag
    11821 cggcaagcgg tgttacatac ccaagacgcg acgggaaatg cttcgcgatt tccgcctgta
    11881 actgcgccat attcatattc tgcaactggc gaatgcgtgc cggagagtca taccacacca
    11941 gcgatgccca gttatcaaac aacggcagaa acgcacgagg tccatccggg gtaaattgct
    12001 gccaggtgct gtcgccggga tcgttctcac actgtacgct aattaacata cacgactgtg
    12061 catactgcca ggcatgaacg ccaattcccg ccatctgccg cacctgggaa tttgcgccgt
    12121 cggcaccaat caccagcttc gcgcggatcg tttcaccgcc cttcagttcc agctcctgaa
    12181 gatcattatg gcgatgcagc gcaatcagcg agcctggcac acgtaacgtt actttcggat
    12241 gcgcttccag cgcctgccac agcgcctgtt gcagaacagt gttttccacc atgtagccaa
    12301 gcacaggcag ctttaattcg gctgcatcaa acaccacatg cgccgtttcc cactcccaag
    12361 tttccagtct gcggtaagga tggcaacgca tagcctgtac cgcgtcccag acgcctaaac
    12421 ctttaagcaa tgataccgaa gccgcactga tcgccgagat ccgcacgtcc ggctggctat
    12481 caacaacaaa cgtcgcagat tgggcatgtt cgataaccgt taccgaaaat ccgtgctgag
    12541 ccagtcccag cgccagtgcg ccgccgacca ttcctccgcc gacaatggca atttccgttg
    12601 gttgatttgt catggtcaat catcctgtta gcaatatgct ttaagtttac aggattttta
    12661 cccctaaggg ctgataacgc tcatactggt cacaacggca gcaaagcatt acactatgcg
    12721 ccctgcattc ctggctacta tttcgcaaga gcaagtcgat gaccaaaaaa ctccatatta
    12781 aaacctgggg ctgtcagatg aacgagtacg attcatcgaa gatggccgat ctgctggatg
    12841 ccacccacgg ctatcaactg accgacgtgg cggaagaagc ggatgtgctg ctgctgaaca
    12901 cctgctcaat ccgcgagaag gctcaggaaa aagtcttcca tcagttgggt cgctggaaac
    12961 tgttaaaaga gaagaatcca gacctgatta tcggcgtcgg cggctgcgtg gcatcgcaag
    13021 aaggcgagca cattcgccag cgcgcccact atgtcgatat tatttttggg ccgcaaacgc
    13081 tgcaccgtct gccggagatg atcaactccg tgcgcggcga ccgcagcccg gttgtagata
    13141 tcagcttccc ggaaatcgag aagtttgacc gtctgccgga accgcgtgcc gaagggccga
    13201 ccgcgtttgt ctccatcatg gaaggctgca ataaatattg cacctactgc gtagtgcctt
    13261 acacccgtgg tgaagaggta agccgtccgt ccgacgatat tctgtttgag atcgcccagc
    13321 ttgcggctca gggcgtgcgt gaagttaacc tgctcggcca gaacgtgaac gcctggcgcg
    13381 gtgagaacta cgacggcacc accggatcgt ttgccgatct gctgcgtctg gttgcggcga
    13441 tcgacgggat cgatcgtatt cgctttacca ccagccatcc gatcgaattc accgacgata
    13501 tcatcgaagt gtaccgcgac acgccggagc tggtgagctt cctgcatctg ccggtacaga
    13561 gcggttccga tcgcattctg aacctgatgg gacgtaccca tacagcgctg gagtacaaag
    13621 cgatcatccg taagctgcgt gcggcgcgtc cggatattca gatcagttct gacttcatcg
    13681 ttggcttccc tggcgaaacc accgaagact tcgagaaaac gatgaagctg attgccgacg
    13741 tcaatttcga catgagctac agctttatct tctccgctcg tccaggtaca ccagccgccg
    13801 atatggttga tgatgttccg gaagaagaga agaagcagcg tctgtatatt ctgcaagagc
    13861 gcattaatca gcaagcgatg gcatggagcc gccgaatgct cggcaccacc cagcgtattc
    13921 tggtagaagg tacatcgcgt aagagcatca tggagctttc cggtcgcact gaaaataacc
    13981 gcgtggttaa cttcgaaggt acaccggata tgatcggtaa attcgtcgat gtagaaatta
    14041 ccgacgtcta cccgaactct ctgcgcggta aagtggtgcg tactgaagat gaaatgggtc
    14101 tgcgcgtggc agaaacaccg gaatcggtga ttgcccgtac ccgcaaagaa aacgaccttg
    14161 gcgtgggtta ttatcagccg taattctcag gcccgccgtt ccggtgggcc ttgttttttc
    14221 tctctttatc cccatcttta tcgcaatgct tgcgcctttt tcctgtggcg tgaataattt
    14281 ccttataggc cggtgaatgt tcagcttccg gcggcataca gataagagga acggtttgaa
    14341 catagacact cgcgaaatca ccctggagcc agcagacaat gcgcgtctgt tgagcctgtg
    14401 cggcccgttt gatgacaaca tcaagcagct cgaacgccgt ctcggcatcg agatcaatcg
    14461 ccgcgataac cactttaaac tgaccggccg tccgatttgc gtcaccgctg cggcagacat
    14521 tctgcgtagc ctgtatgtcg ataccgcccc gatgcgcggt cagattcagg atatcgaacc
    14581 ggaacagatc caccttgcga ttaaagaagc gcgggtactg gagcaaagcg cggagagcgt
    14641 gccggagtac ggcaaagcgg tcaatatcaa gaccaaacgc ggcgtaatca agccgcgcac
    14701 accaaaccag gcgcagtaca ttgccaatat tctcgaccat gacatcacct tcggcgttgg
    14761 cccggcgggt acgggtaaaa cctacctggc agtggctgcg gcagttgatg ctctggagcg
    14821 tcaggaaatt cgccgtattc tgctgactcg tccggcggta gaagccggtg agaaactggg
    14881 cttcctgcct ggcgatttaa gccagaaagt agatccgtat ctgcgcccgc tgtacgacgc
    14941 gctgtttgaa atgctgggct ttgagaaagt cgagaaactg attgagcgca acgttattga
    15001 agtcgcaccg ctggcctata tgcgtggccg tacgctgaac gacgcgttta tcattcttga
    15061 tgaaagccag aacaccacca tcgaacagat gaagatgttc ctgacccgta tcggttttaa
    15121 ctcaaaagcg gttatcaccg gcgacgtcac acagatcgac ctgccgcgta atactaaatc
    15181 aggcttacgt cacgccatcg aagtgctggc cgatgttgaa gagatcagct ttaacttctt
    15241 ccacagcgaa gacgtggttc gtcacccggt ggtggcgcgt atcgttaacg cttatgaagc
    15301 ctgggaagaa gcagaacaaa aacgtaaagc ggcgctggca gcagaacgca agcgcgaaga
    15361 acaggaacaa aaatgagtca ggtgatcctc gatttacaac tggcatgtga agataattcc
    15421 gggttaccgg aagagagcca gtttcaaaca tggctgaatg cggtgatccc gcagtttcag
    15481 gaagagtcgg aagtgactat tcgcgtggtc gataccgccg aaagtcacag tctgaatctg
    15541 acctatcgcg gtaaggataa gccgaccaac gtgctctcct tcccgtttga agtgccgcct
    15601 ggcatggaaa tgtcgctact gggcgatctg gttatctgcc gtcaggtggt tgagaaggaa
    15661 gctcaggagc aaggcaaacc actggaggcg cactgggcgc atatggtggt gcacggcagt
    15721 ctgcatttgt taggttacga tcacatcgaa gatgacgaag cagaagaaat ggaagccctc
    15781 gaaacagaga ttatgcttgc tctgggctat gaggatccgt acattgccga gaaagaataa
    15841 tttgccaggc atttgactgg cagacgtccc cgccgttggt gattaacgac ggcgttttga
    15901 ttaactaaca tgagaaccca tacgacgcca tgagcgacga caattcacac agtagtgaca
    15961 cgataagcaa caagaaggga tttttctccc tgttactcag ccaacttttc cacggtgaac
    16021 cgaaaaaccg tgacgaactg ctggcgctga tccgtgattc cgggcagaac gaccttatcg
    16081 acgaagatac gcgcgatatg ctcgaagggg tgatggacat cgcagaccaa cgcgtccgcg
    16141 acatcatgat cccccgctcc cagatgatta ccctgaaacg caaccagacg ctggacgaat
    16201 gccttgatgt catcatcgag tccgcccact cacgtttccc ggtgattagc gaagacaaag
    16261 atcacattga agggattctg atggcgaaag acttgctgcc gtttatgcgc agcgatgctg
    16321 aagccttcag catggacaaa gtgttacgtc aggcagttgt cgttcctgaa agtaagcgcg
    16381 tagaccggat gctgaaagag tttcgctctc agcgttacca catggcgatc gtcattgacg
    16441 aattcggtgg ggtttccggt ctggtgacca ttgaagacat cctggaactg attgttggtg
    16501 agattgaaga cgagtatgac gaagaagatg atatcgactt tcgtcagctg agtcgccata
    16561 cctggaccgt gcgcgcactg gcttccattg aagacttcaa cgaagcgttc ggcacccact
    16621 ttagcgatga agaggttgac actatcggtg gtctggtgat gcaggcattt gggcatcttc
    16681 cggcgcgtgg cgaaaccatc gacatcgacg gttaccagtt caaagtggcg atggccgaca
    16741 gtcggcgtat tattcaggtt catgtcaaaa tcccggatga ctcaccccag ccgaagctgg
    16801 atgaataaaa ccgaaactgg atagataact acatggcttt tgcctcatta attgaacgcc
    16861 agcgcattcg cctgctgctg gcgttattat tcggtgcctg cggaacgctg gccttctctc
    16921 cttacgacgt ctggcctgcg gcgattattt cgctgatggg gcttcaggcg ttgaccttta
    16981 accgtcgtcc actccagtct gccgctattg gcttttgctg gggatttggt ctctttggca
    17041 gcggtattaa ctgggtctat gtcagcatcg cgacctttgg tggaatgcct ggcccggtta
    17101 acgtcttcct ggtggtgctg ctggcggcgt atttgtcgct gtataccgga ctgtttgctg
    17161 gcgtgctgtc gcgtctgtgg ccgaaaacca cctggctgcg cgtggcgatt gccgcccctg
    17221 ctctctggca agtgaccgag tttctgcgcg gttgggtgct gactggcttc ccgtggttac
    17281 agttcggcta tagccagatt gatggcccgt taaaagggct ggcaccgata atgggcgtgg
    17341 aagccattaa cttcctgctg atgatggtta gcggcctgct ggcactggcg ttggtcaaac
    17401 gcaactggcg tccgctggcg gtggccgtcg tgctgtttgc ccttcccttc ccgctgcgtt
    17461 acatccaatg gtttaccccg caaccggaaa aaactattca ggtttcgatg gttcagggcg
    17521 atattccgca atcgctgaaa tgggacggag accagctact taatacgctg aagatttact
    17581 acaacgcaac ggcaccgctg atgggcaaat catcgttgat tatctggccg gagtcggcga
    17641 taaccgatct ggaaattaat cagcaaccgt tcctcaaagc actggacggt gagttgcgcg
    17701 ataaagggag ctcgctggta accgggattg ttgacgcgcg tctcaataag cagaaccgct
    17761 acgatactta caacaccatc atcacgctgg ggaaaggtgc gccgtacagc tacgaatcag
    17821 ccgatcgcta taacaaaaac catctggtgc cgtttggcga gtttgtcccg ctggagtcga
    17881 ttctgcgtcc gttagcgccg ttctttgatc tgccgatgtc gtcgttcagc cgtgggccat
    17941 atatccagcc accgctgtcg gtaaacggta ttgagcttac tgcggctatt tgctacgaga
    18001 tcattctcgg cgagcaagtg cgtgataact tccgcccgga taccgattat ctgctgacta
    18061 tctccaacga tgcatggttt ggtaagtcta ttggtccatg gcagcacttc cagatggcgc
    18121 gaatgcgtgc gctggagttg gcgcgcccgc tactgcgtag caccaacaac ggcattacgg
    18181 cggtgattgg tccgcagggt gagattcagg cgatgatccc gcagttcacc cgcgaagtgt
    18241 tgaccactaa tgtgactccg accaccggac tcacaccgta cgcacgtacc ggcaactggc
    18301 cgctgtgggt actgacggca ttgtttggtt ttgctgctgt gctgatgagc ctgcgtcaac
    18361 gacgtaaata atcccttctt atcgtgccgg aatacatccc gtatttcggc acgctcaccg
    18421 gatgcggcat cagtgcgtta tccggataac aaaattcccc tatccaactt tggcacatct
    18481 attgctttgt tatacaaggc aaagcttgaa ccagcatcag tacaacccgg ttgcaccaca
    18541 gcaatccacg ggtagcacca aaagagtgca ttttaagaat tttgcttcgg aatggtgcgg
    18601 cgcatctcgc aaaaacaaac aataaaacag caatatcttt acatttaacc aaactaaatg
    18661 ttacctaaca ggcacaacac tgcacaataa agttgcagac gataacaaca caaacactca
    18721 caacaggtat ccatgcgttc ttaacgcaga agataaagga gttggatatg caattacgta
    18781 aacctgccac agcaatcctc gccctggcgc tttccgcagg actggcacag gcagatgacg
    18841 ccgccccggc agcgggcagt acgctggaca aaatcgccaa aaacggtgtg attgtcgtcg
    18901 gtcaccgtga atcttcagta cctttctctt attacgacaa tcagcaaaaa gtggtgggtt
    18961 actcgcagga ttactccaac gccattgttg aagcagtgaa aaagaaactc aacaaaccgg
    19021 acttgcaggt gaaactgatt ccgatcacct cacaaaaccg tattccactg ctgcaaaacg
    19081 gcactttcga ttttgaatgt ggttccacca ccaataacgt cgaacgccaa aaacaggcgg
    19141 ctttctctga cactattttc gtggtcggta cgcgcctgct gaccaaaaag ggcggcgata
    19201 tcaaagattt tgccgacctg aaagacaaag ccgtagtcgt cacttccggc actacctctg
    19261 aagttttgct caacaaactg aatgaagagc aaaaaatgaa tatgcgcatc atcagcgcca
    19321 aagatcacgg tgactctttc cgcaccctgg aaagcggtcg tgccgttgcc tttatgatgg
    19381 atgatgccct gctggccggt gaacgtgcga aagcgaagaa accagacaac tgggaaatcg
    19441 tcggcaagcc gcagtctcag gaagcctacg gttgtatgtt gcgtaaagat gatccgcagt
    19501 tcaaaaagct gatggatgac accatcgctc aggtgcagac ctccggtgaa gcggaaaaat
    19561 ggtttgataa atggttcaaa aatccaattc cgccgaaaaa cctgaacatg aatttcgaac
    19621 tgtcagacga aatgaaagca ctgttcaaag aaccgaatga caaagcactg aactaattac
    19681 aagaaccagg ggcggaaatt ccagccctct cgattgttac gtagcacgga cagactatac
    19741 gcctgatggt cgttccccat cgggcctgaa aaccgcaata cgctgggtaa caatcttcga
    19801 gggtagcagt taacgctgct accctctttt ttctggagta gatttatgtc tatagactgg
    19861 aactggggta tttttttaca acaagccccg ttcggcaaca ccacctatct tggttggatc
    19921 tggagtggtt ttcaggtgac gatcgctttg tcgatctgcg cctggattat cgctttcctc
    19981 gtcggttcat ttttcggcat tttacgtacc gttccaaacc gttttctctc cggtctgggt
    20041 acgttgtatg tcgaactgtt ccgcaacgtg ccgttgattg tgcaattctt tacctggtat
    20101 ctggtgatcc cggagctgct gccggagaaa atcggcatgt ggtttaaggc cgagctggat
    20161 cccaatattc agtttttcct ttcatccatg ctctgcctgg ggctgtttac cgccgcccgt
    20221 gtttgcgaac aggtacgcgc cgcgatccag tcgctgccgc gagggcaaaa aaatgccgcg
    20281 ctggcgatgg gcctgacgct accgcaggct tatcgatacg tactgttgcc taatgcttat
    20341 cgcgttatcg tcccgccgat gacctccgag atgatgaacc tggtgaaaaa ctctgccatc
    20401 gcctcgacta ttggtctggt ggatatggcg gcgcaggcgg gtaaattgct tgattactca
    20461 gcccacgcct gggagtcgtt taccgccatc acgctggcgt atgtattgat taacgctttt
    20521 atcatgctgg tgatgacgct ggttgaacgt aaagttcgcc tgcctggcaa catggggggc
    20581 aaataatgta cgagtttgac tggagttcca ttgtcccttc cctgccatat ctgctcgacg
    20641 ggctggtgat cacgctgaaa atcaccgtca cagcagtcgt gattggtatt ttgtggggca
    20701 ccatgctggc ggtgatgcgc ttgtccagct ttgcgcccgt agcgtggttt gccaaagcct
    20761 acgttaacgt attccgctcg attcctttag ttatggtttt gctgtggttt tacctgatcg
    20821 tgccgggttt tctgcaaaac gtactgggat tatcaccaaa aaatgacatc cgtttaattt
    20881 cggcgatggt ggcgttttcg atgtttgaag cagcctacta ttcagagatt atccgtgccg
    20941 gtattcaaag tatttcccgt ggtcagtcga gcgccgcgct ggcgttgggg atgactcact
    21001 ggcagtcgat gaaactgatt attctgccgc aggctttccg cgcgatggtg ccgctgctgc
    21061 tcactcaggg catcgtactg ttccaggata cctcactggt gtatgtgtta tctctggccg
    21121 atttcttccg taccgcctca accattggtg agcgtgatgg tacgcaggtc gagatgatcc
    21181 tgtttgccgg atttgtttat ttcgttatta gccttagcgc gtcgttgttg gtcagctact
    21241 tgaaaagaag gacagcttaa tgattaccct gaaaaatgtt tcaaaatggt atggtcactt
    21301 tcaggtgctg accgactgct caaccgaagt gaaaaaaggc gaagtggtgg tggtttgcgg
    21361 cccgtctggt tccggcaaat caacgctgat taaaaccgtc aacggcctcg aaccggtgca
    21421 gcaaggtgaa attaccgtcg atggcatcgt ggttaacgat aagaaaaccg atctggcaaa
    21481 gctgcgttcc cgcgtcggga tggtgttcca gcatttcgag ttgttccccc atttgtcgat
    21541 tattgaaaac ctgaccctgg cgcaggtgaa agtgcttaaa cgcgataaag cacccgctcg
    21601 tgaaaaagca ttgaaattgc tggagcgcgt cgggctttcc gctcatgcca ataagtttcc
    21661 ggcgcagctt tccggcggtc agcagcagcg tgtggcgatc gctcgcgcat tgtgcatgga
    21721 tcctatcgcg atgctgttcg acgaaccgac atcggcgctg gatccggaga tgatcaacga
    21781 agtgctggac gtgatggtgg aactggcgaa cgaaggaatg accatgatgg tggtgaccca
    21841 cgaaatgggc tttgcccgta aagtggcgaa tcgggtgatc tttatggacg agggtaaaat
    21901 tgtcgaagac tcgccgaaag acgctttctt cgatgatccg aaatcggacc gcgcaaaaga
    21961 cttcctcgcg aaaatcctgc attaatcact ctggcgcgca tacccaagtg cgcgccatct
    22021 cacgctttat cgtttcactc ctcgtatctg ttacatcctg gcgttaccct tgtcgcaaag
    22081 aagcacacaa caaggagcaa caatggcact gccaattctg ttagattgcg acccaggtca
    22141 tgacgacgct atcgcaatag ttcttgccct cgcctcacca gagcttgatg tcaaagcaat
    22201 tacgtcttcc gccggaaacc agactccaga aaaaacctta cgcaatgttc tgcgtatgct
    22261 gaccttgctt aatcgcaccg atattccggt agcaggcggc gcggtaaaac cgttaatgcg
    22321 tgagttgata atcgcggaca atgtgcacgg cgaaagcggt ctcgacggcc cggcattacc
    22381 ggaaccgacg ttcgcaccgc aaaactgtac ggcggtggag ctgatggcga aaacgctgcg
    22441 tgaaagtgcg gaacctgtca cgattgtgtc taccggaccg caaactaacg ttgccttgct
    22501 gctcaatagc catccggaac tgcatagcaa aattgcccgt atcgtgatta tgggcggcgc
    22561 aatggggctt ggtaactgga cgccagccgc cgagtttaat atttatgttg acccggaagc
    22621 ggcagaaatt gttttccagt cagggatccc ggtggtgatg gccggtctgg atgttactca
    22681 caaagcacaa attcacgttg aggacaccga gcgtttccgc gcgattggca accctgtttc
    22741 aaccattgtt gccgaactgc tggatttctt cctcgaatat cataaagacg aaaaatgggg
    22801 ctttgtcggc gcaccgctgc atgacccatg caccatcgcc tggctgttaa acccggagtt
    22861 gtttaccact gttgagcgct gggttggcgt ggaaacacaa gggaaatata cccagggtat
    22921 gacggttgtt gattattatt atctgaccgg caataaaccg aatgctactg taatggtcga
    22981 tgttgatcgt cagggccttg ttgatttact ggctgatcgt ctgaaatttt acgcttaatc
    23041 actcgccgga tgacatcacg ccatccggca ccactcattt cattaaggct cgaacgggcg
    23101 tctctggaac tggtcatgac cacatttcgg gcatagcgtc agcacttccg gtgtgtagat
    23161 cgggagatgg aaatgacatt tctcgcagac cagattaccc agccctacca cttctccgct
    23221 gtgataaacc ccgtgatgat tgaggtcctg gaaaacttcg cgccattcaa gctgcgtttt
    23281 atcggtgatg tctgccagct cctgccacaa gctttcttta atcacccgca taaagacgct
    23341 gtcagactct tctttcaggc tctcttcata gctcatggcg aactcttcca ggtcacgtct
    23401 gacagctcgc gtcagctcat cgacttcggt tcgcgttaac tcccctgttt ttattacgcg
    23461 ctcgcgcgcc tgttccacca gtgcgtcgat atcacgttcg ccattgcgca ggcgttcgct
    23521 cagtgacgcg accagttcac ggtaatattg agcaaccttg ttcatcgttt catctcccgg
    23581 gtcgttaact gactataata atagacgttc tttgttcctt gctttgctaa tacgcccaca
    23641 aactctgtaa attaatgtat gaataatccg ggcatcattt caacgtcgaa tgcgcgaaag
    23701 gctgttttga cgcggacgtt tgggctatgc tatgcggatc tgaaaaacca cctcaacgct
    23761 acatttgtag ccgtattgaa aacaggacca ctggctgcca tgcaagagca ataccgcccg
    23821 gaagagatag aatccaaagt acagcttcat tgggatgaga agcgcacatt tgaagtaacc
    23881 gaagacgaga gcaaagagaa gtattactgc ctgtctatgc ttccctatcc ttctggtcga
    23941 ctacacatgg gccacgtacg taactacacc attggtgacg tgatcgcccg ctaccagcgt
    24001 atgctgggca aaaacgtcct gcagccgatc ggctgggacg cgtttggtct gcctgcggaa
    24061 ggcgcggcgg tgaaaaacaa caccgcaccg gcaccgtgga cgtacgacaa catcgcgtac
    24121 atgaaaaacc agctcaaaat gctgggcttt ggttatgact ggagccgcga gctggcaacc
    24181 tgtacaccgg aatactaccg ttgggaacag aaattcttca ccgagctgta taaaaaaggc
    24241 ctggtatata agaagacttc tgcagtcaac tggtgcccga acgaccagac cgtactggcg
    24301 aacgaacaag ttatcgacgg ctgctgctgg cgctgcgata ccaaagttga gcgtaaagag
    24361 atcccgcaat ggtttatcaa aatcaccgct tacgctgacg agctgcttaa cgatctggat
    24421 aaactggatc actggccaga caccgttaaa accatgcagc gtaactggat cggtcgttcc
    24481 gaaggcgtgg aaatcacctt caacgttaac gactatgaca acacgctgac cgtttacact
    24541 acccgcccgg acacctttat gggttgtacc tacctggcgg tagcggcagg tcatccgctg
    24601 gcgcagaaag cggcggaaaa taatcctgaa ctggcggcct ttattgacga atgccgtaac
    24661 accaaagttg ccgaagctga aatggcaacg atggagaaaa aaggcgtcga tactggcttt
    24721 aaagcggttc acccattaac gggcgaagaa attcccgttt gggcagcaaa cttcgtattg
    24781 atggagtacg gcacgggcgc agttatggcg gttccgggtc acgaccagcg cgactacgag
    24841 tttgcctcta aatacggcct gaatatcaaa ccggttatcc tggcagctga cggctctgag
    24901 ccagatcttt ctcagcaagc cctgactgaa aaaggcgttc tgttcaactc tggcgagttc
    24961 aacggtcttg accatgaagc ggccttcaac gctatcgccg ataaactgac tgcgatgggc
    25021 gttggcgagc gtaaagtgaa ctaccgcctg cgcgactggg gtgtttcccg tcaacgttac
    25081 tggggcgcgc cgattccgat ggtgacgctg gaagacggta ccgtaatgcc gaccccggac
    25141 gaccagctgc cggtgatcct gccggaagat gtggtgatgg acggtattac cagcccgatt
    25201 aaagcagatc cggagtgggc aaaaactacc gttaacggta tgccagcgct gcgtgaaacc
    25261 gacactttcg acacctttat ggagtcctcc tggtactatg cgcgctacac ttgcccggag
    25321 tacaaagaag gtatgctgga ttccaaagcg gctaactact ggctgccggt ggatatctac
    25381 attggtggta tcgaacacgc cattatgcac ctgctctact tccgcttctt ccacaaactg
    25441 atgcgtgatg caggcatggt gaactctgac gaaccagcga aacagttact gtgtcagggt
    25501 atggtgctgg cagatgcctt ctactatgtt ggcgaaaacg gcgaacgtaa ctgggtttcc
    25561 ccggttgatg ctatcgttga acgtgacgag aaaggccgta tcgtgaaagc gaaagatgcg
    25621 gcaggccatg aactggttta taccggcatg agcaaaatgt ccaagtcgaa gaacaacggt
    25681 atcgacccgc aggtgatggt tgaacgttac ggcgcggaca ccgttcgtct gtttatgatg
    25741 tttgcttctc cggctgatat gactctcgaa tggcaggaat ccggcgtgga aggggctaac
    25801 cgcttcctga aacgtgtctg gaaactggtt tacgagcaca cagcgaaagg tgatgttgcg
    25861 gcactgaacg ttgatgcgct gactgaagat cagaaagcgc tgcgtcgcga tgtgcataaa
    25921 acgatcgcta aagtgaccga tgatatcggc cgtcgtcaga ccttcaacac cgcaattgcg
    25981 gcgattatgg agctgatgaa caaactggcg aaagcaccga ccgatggcga gcaggaccgc
    26041 gccctgatgc aggaagcgct gctggcggta gtccgtatgc ttaacccgtt caccccgcac
    26101 atctgcttca cgctgtggca ggaactgaaa ggcgaaggcg atatcgacaa cgcaccgtgg
    26161 ccggttgctg acgaaaaagc gatggtggaa gactccacgc tggtcgtggt gcaggttaac
    26221 ggtaaagtcc gtgcaaaaat caccgttccg gtggacgcaa cggaagaaca ggttcgcgaa
    26281 cgtgctggcc aggaacatct ggtagcaaaa tatcttgatg gcgttactgt acgtaaagtg
    26341 atttacgtac ccggtaaact cctcaatctg gtcgttggct aagcgcggga ggaagcgtgc
    26401 gatatctggc aacattgttg ttatctctgg cggtgttaat caccgccggg tgtggctggc
    26461 atctgcgtga taccacgcag gttccttcca ctatgaaggt catgatcctg gactcaggcg
    26521 atccgaacgg gccattaagc cgtgcggtgc gtaaccagtt acgtctgaat ggtgtcgagt
    26581 tgcttgataa agaaaccacg cgtaaggacg ttccatccct gcgtttgggt aaagtgagca
    26641 tcgcgaaaga taccgcatcg gtattccgta acggtcaaac agcagagtat cagatgatca
    26701 tgacggtcaa tgcgaccgtg ttgatccccg gccgtgacat ctacccgatt agcgccaaag
    26761 tcttccgttc gttcttcgat aacccgcaaa tggcgttagc gaaagataac gaacaagaca
    26821 tgatcgtaaa agagatgtac gaccgtgctg ccgaacagct gattcgtaag ctgccaagca
    26881 tccgtgctgc ggatattcgt tccgacgaag aacagacgtc gaccacaacg gatactccgg
    26941 caacgcctgc acgcgtctct accacgctgg gtaactgatg attcggttgt acccggaaca
    27001 actccgcgcg cagctcaatg aagggctgcg cgcggcgtat cttttacttg gtaacgatcc
    27061 tctgttattg caggaaagcc aggacgctat tcgtcaggta gctgcggtac aaggattcga
    27121 agaacaccac actttttcca ttgatcccaa cactgactgg aatgcgatct tttcgttatg
    27181 ccaggccatg agtctgtttg ccagtcgaca aacgctattg ctgttgttat cagaaaacgg
    27241 accgaatgcg gcgattaatg aacaacttct cacactcacc ggacttctgc atgacgacct
    27301 gctgttgatc gtccgcggca ataaattaag caaagcgcaa gaaaatgccg cctggtttac
    27361 tgctcttgcg aatcgcagcg tgcaggtgac ctgtcagaca ccggagcagg ctcagcttcc
    27421 ccgttgggtt gctgcgcgtg caaaacagct caacttagaa ctggatgacg cggcgaatca
    27481 ggtgctctgc tactgttatg aaggtaacct gctggcgctg gctcaggcac tggagcgttt
    27541 atcgctactc tggccagacg gcaaactgac attacctcgc gttgaacagg cggtgaatga
    27601 tgccgcgcat ttcacccctt ttcattgggt tgatgctttg ttgatgggaa aaagtaagcg
    27661 cgcgttgcat attcttcagc aactgcgtct ggaaggcagc gaaccggtta ttttgttgcg
    27721 cacattacaa cgtgaactgt tgttactggt taacctgaaa cgccagtctg cccatacgcc
    27781 actgcgtgcg ttgtttgata agcatcgggt atggcagaac cgccggggca tgatgggcga
    27841 ggcgttaaat cgcttaagcc agccgcagtt acgtcaggcc gtgcaactcc tgacacgaac
    27901 ggaactcacc ctcaaacaag attacggtca gtcagtgtgg gcagagctgg aagggttatc
    27961 tcttctgttg tgccataaac ccctggcgga cgtatttatc gacggttgat atgaaatctt
    28021 tacaggctct gtttggtggc acctttgatc cggtgcacta tggtcatctt aaacccgtgg
    28081 aaacgctggc gaatttgatt ggtctgacgc gggtcacaat catccctaat aatgttcctc
    28141 cgcatcgtcc ccagccggaa gcgaacagcg tacagcgtaa acacatgctt gaactggcga
    28201 ttgccgacaa gccattattt actcttgatg aacgcgagct aaagcgcaat gccccctctt
    28261 acactgcgca aacactgaaa gagtggcggc aggaacaagg gccggacgtg ccgctggcgt
    28321 ttattatagg tcaggattca ctgctgacct ttccgacctg gtacgaatac gaaacgatac
    28381 tcgacaatgc acatttgatc gtctgtcggc gtccaggtta cccacttgaa atggcgcaac
    28441 cgcaatacca gcaatggctg gaagatcatt tgacacataa cccggaagat cttcaccttc
    28501 agcctgccgg taaaatttat ctggctgaaa cgccgtggtt taacatctcg gcgaccatta
    28561 tccgcgaacg tttgcaaaac ggtgaatcgt gtgaggattt attgccggaa ccggtactga
    28621 cttacattaa ccaacaaggc ttgtatcgct gatacctgtc gttacggagg gcatgatgcg
    28681 actgtggtta attcgtcatg gtgaaacgca agcgaatatc gatggtctgt acagcggtca
    28741 tgctcccaca cccctgactg cgcgcggtat tgagcaagcg caaaatctgc atacgctgct
    28801 acatggtgtt tcctttgacc tggttttatg cagtgaactg gaacgtgcac agcataccgc
    28861 gcgacgggtt ctcagtgatc gccagcttcc cgtgcaaatc atccctgaac tcaacgaaat
    28921 gttttttggc gactgggaga tgcgacatca tcgcgacctc atgcaagaag atgccgaaaa
    28981 ctatagcgcg tggtgcaatg actggcagca tgctatcccc acgaacggtg aaggatttca
    29041 ggcattttca caacgagtgg aacggtttat cgcaaggctt agtgaatttc agcactatca
    29101 gaatatttta gtcgtcagcc atcagggtgt actgagtctg ttaatcgccc gtttaattgg
    29161 tatgcctgcc gaagctatgt ggcattttcg cgttgaccag gggtgttgga gcgccattga
    29221 tatcaaccaa aaattcgcga cgctacgcgt cctcaatagc cgtgccatcg gggtcgagaa
    29281 tgcatgactt ttctgttttt ttacgggtaa gccgcaacga ccattgacag ccccgggcag
    29341 gctgatattc tccgcagcca gacttttttc gccagacacg actttgtaga aattgtttta
    29401 caaaaatggc gatgcaatct gcggcgcggg gtgggatgat agcccacttt cgaaagccga
    29461 ttcggcgaca attgtcccga aatcgcctct ggttcaggta tactgacaga ccatttttat
    29521 ctatttgatt cacccagggg gaaaacttgc agggtaaagc actccaggat tttgttatcg
    29581 acaaaattga tgacctcaaa ggtcaggaca tcatcgcctt agacgttcag ggcaaatcca
    29641 gcatcaccga ctgcatgatc atctgtacgg gtacgtccag ccgtcatgtt atgtccattg
    29701 ctgaccacgt tgtgcaggag tctcgcgcag cgggcctgtt accgctcggc gtagaaggtg
    29761 aaaacagcgc cgactggatt gtcgtggatt tgggcgatgt gattgtccat gtcatgcagg
    29821 aagagagccg tcgcctgtat gaactggaaa aactctggag ttaatgcgtg aagctgcaac
    29881 ttgtcgccgt gggaacgaaa atgccggact gggtacaaac tggttttacc gagtacctgc
    29941 gtcgttttcc gaaagatatg cccttcgagc tgattgaaat tccggccgga aaacgcggca
    30001 agaatgcgga catcaagcgc atactcgaca aagagggtga gcagatgttg gcggccgcag
    30061 gcaaaaaccg cattgtcacc ctcgatattc caggcaagcc ctgggatacg ccgcagttag
    30121 ccgctgagct ggaacgctgg aagctggatg gtcgcgacgt cagtctactg attggcgggc
    30181 ctgaagggtt gtcgcctgcc tgtaaagcgg cggcggagca gagctggtcg ctgtcggcgc
    30241 ttaccctccc ccatccgttg gttcgcgtgc tggtcgcaga gagtctgtac cgggcgtgga
    30301 gcatcaccac caaccatcct tatcaccgtg agtgataagg gagctttgag tagaaaacgc
    30361 agcggatgaa actacagaac tcttttcgcg actatacggc agagtccgcg ctgtttgtgc
    30421 gccgggcgct ggtcgccttt ttggggattt tgctgctgac cggcgtgctt atcgccaacc
    30481 tgtataatct gcaaattgtt cgctttaccg actaccagac ccgctctaat gaaaaccgca
    30541 ttaagctggt gcctatcgcg cccagccgcg gcattatcta cgaccgtaac ggtatccctc
    30601 tggccctcaa ccgcactatc taccagatag aaatgatgcc ggagaaagtc gataacgtgc
    30661 agcaaacgct ggacgctttg cgcagcgtgg ttgatctgac cgatgacgat attgctgcat
    30721 tccgaaaaga gcgcgcacgt tcacaccgtt tcacctccat tccggtgaaa actaacctga
    30781 ccgaagtaca agtagctcgc tttgccgtca atcagtaccg ttttccgggt gttgaagtta
    30841 aaggctataa acgtcgttac tatccttacg gttcggcgtt gacccacgtc atcggctatg
    30901 tgtcgaaaat caacgataaa gacgtcgaac gcctgaataa tgacggcaaa ctggccaact
    30961 atgcggcaac gcatgatatc ggtaagctgg gcattgagcg ttactatgaa gacgtgctgc
    31021 acggtcagac cggttatgaa gaggttgaag ttaacaaccg tgggcgtgtt atccgccagt
    31081 taaaagaagt gccaccgcaa gccggacacg atatttacct gacgctggat ctcaaactcc
    31141 agcaatatat tgaaacgctg ctggcgggta gccgcgcagc ggtggtagtc accgatccgc
    31201 gtacaggtgg ggtgctggcg ctggtttcca cgcctagtta tgacccaaac ctgtttgttg
    31261 acggtatctc cagcaaagat tattccgcct tgctgaacga cccgaataca ccgctggtga
    31321 accgcgctac acagggggtt tatcctcccg cgtctacagt taaaccctat gtggcggttt
    31381 cggcattgag cgccggagtg atcacgcgca atacgacgct gtttgaccca ggctggtggc
    31441 aactgccagg ttcggaaaaa cgttatcgtg actggaaaaa atggggccac gggcgtctga
    31501 atgtcacaag atcgctggaa gaatctgcgg ataccttctt ctatcaggtg gcctacgata
    31561 tgggaatcga tcgcctctcc gaatggatgg gtaaattcgg ttatggtcat tacaccggta
    31621 tcgacctggc ggaagaacgt tccggcaaca tgcctacccg cgaatggaaa cagaaacgct
    31681 ttaaaaaacc gtggtatcag ggtgacacca ttccggttgg tatcggtcag ggttactgga
    31741 cagcgacccc aatccagatg agtaaggcac tgatgatcct gattaatgac ggtatcgtga
    31801 aggttccgca tttgctgatg agcaccgccg aagacggcaa acaggtgcca tgggtacaac
    31861 cgcatgaacc gcccgtcggc gatattcatt ccggttactg ggagctggcg aaagacggta
    31921 tgtacggtgt tgctaaccgc cctaacggta cggcgcataa atactttgcc agcgcaccgt
    31981 acaaaattgc ggcgaaatcc ggtaccgctc aggtcttcgg tctgaaagcg aacgaaacct
    32041 ataatgcgca caaaattgcc gagcgtttac gtgaccacaa actgatgacc gcctttgcgc
    32101 catacaacaa tccgcaagtc gctgtcgcca tgattctgga gaacggcggt gcgggtccgg
    32161 cggttggtac gctgatgcgc cagatcctcg accacattat gctgggtgat aacaacaccg
    32221 atctgcctgc ggaaaatcca gcggttgccg cagcggagga ccattaatca tgacggataa
    32281 tccgaataaa aaaacattct gggataaagt ccatctcgat cccacaatgc tgctgatctt
    32341 actggcattg ctggtttaca gcgccctggt tatctggagc gccagcggtc aggatattgg
    32401 catgatggag cgtaaaatcg gccaaatcgc gatgggtctg gtcatcatgg tggtgatggc
    32461 gcaaattcct ccacgcgttt atgaaggctg ggccccctat ctctatatca tctgtattat
    32521 tttgctggtg gcggtagatg ctttcggtgc catctctaaa ggcgctcaac gctggctgga
    32581 cctcggtatt gttcgttttc agccgtcgga aattgccaaa atagccgtac cactgatggt
    32641 tgcgcgcttt atcaaccgcg acgtttgccc gccatcgttg aagaacacag gcatcgcgct
    32701 agtgctgata tttatgccca cgctgctggt ggctgcacag cctgacctgg ggacatcaat
    32761 cctcgttgcg ctttccggtc tgtttgtact gttcctctct ggccttagct ggcgtctgat
    32821 tggcgtcgca gtagtgctgg tagcggcgtt cattccgatt ctgtggttct tcctgatgca
    32881 tgattaccag cgccagcgcg taatgatgct cctggacccg gaatcagacc cactcggcgc
    32941 gggctatcac attattcagt ctaaaattgc tattggctcc ggcggattac gcggcaaagg
    33001 ctggctgcat ggcactcagt cacaacttga atttctcccc gaacgccata ccgactttat
    33061 cttcgcggta ctggcggaag agctgggatt agtgggcatt ttgattctgc tcgctctcta
    33121 cattctgctg atcatgcgcg ggctgtggat agccgccaga gcgcaaacca cctttggtcg
    33181 cgtcatggct ggcggcttaa tgctgatatt attcgtttat gtcttcgtaa atattggtat
    33241 ggtaagcggt attctgccgg ttgtaggggt tccgctccca ctggtcagtt atggaggatc
    33301 ggcgctaatt gtgctgatgg ctgggttcgg gattgtaatg tcaatccaca cccacaggaa
    33361 aatgttgtcg aaaagcgtgt aagaggtgcg caatgcgtaa gcagtggctc gggatctgca
    33421 tcgcggcagg aatgctcgcg gcatgtacaa gcgatgatgg tcagcaacag acggtaagtg
    33481 taccgcagcc tgcggtatgt aacggcccca tagttgaaat tagcggggcg gacccgcgtt
    33541 tcgaaccact gaacgcgacg gcaaatcagg attaccagcg cgacggtaaa agctacaaaa
    33601 tcgtgcagga tccgtctcga tttagccagg cgggactggc ggcaatctat gatgccgaac
    33661 ccggcagtaa cctgacggcc tctggcgaag cattcgatcc gacacagctg acagcggccc
    33721 atccaacgct tccgatcccc agctacgcca gaatcactaa cctggctaac gggcgaatga
    33781 tcgtggtgcg cattaatgat cgcggtcctt acggcaacga ccgcgttatt tcgctttctc
    33841 gcgcggcagc tgaccgtctt aacacgtcaa acaacaccaa agttcgtatc gatccgatta
    33901 ttgtcgccca ggatggttcg ctttctggtc ctggtatggc ctgtaccaca gttgccaaac
    33961 agacttacgc cctgcctgca cctcccgatt taagcggcgg cgcgggaaca aattcagtgt
    34021 ctggcccgca gggtgacatt cttccggtca gtaattcgac gctaaaaagc gaagatccga
    34081 ccggcgcgcc ggtaaccagc agcggtttcc tcggcgcacc aacgacctta gcgcctggtg
    34141 ttctggaagg cagcgaacca acacctgcac cacagcccgt tgttacagct ccggcgacaa
    34201 cgcctgcaac cacgcctgca atggtgacac cgcaagccac ctcgcaaagc gccagcggca
    34261 actttatggt gcaagtcgga gccgttagcg atcaggctcg tgcgcaacag taccaacagc
    34321 aactgggaca gaagttcggc gtccccggtc gcgtaactca aaatggcgca gtctggcgga
    34381 tccagcttgg cccattcgcc agcaaagccg aagccagtac cttgcagcaa cgtttgcaaa
    34441 ccgaagccca attacagtca tttattacca ccgcgcagta gcgtaaagca ggcatctgat
    34501 gtgtcaattt ctgtaaatga cgttaacaaa gtcacgcgga aagtcagatg cctgccggta
    34561 gtgcatttgc tatagtaggg cacttttttt aattccatca cggatgtcgt agttcagacc
    34621 atgaatacca ttttttccgc tcgtatcatg aagcgcctgg cgctcaccac ggctctttgc
    34681 acagccttta tctctgctgc acatgccgat gacctgaata ttaaaactat gatcccgggt
    34741 gtaccgcaga tcgatgcgga gtcctacatc ctgattgact ataactccgg caaagtgctc
    34801 gccgaacaga acgcagatgt ccgccgcgat cctgccagcc tgaccaaaat gatgaccagt
    34861 tacgttatcg gccaggcaat gaaagccggt aaatttaaag aaactgattt agtcaccatc
    34921 ggtaacgacg catgggctac cggtaacccg gtgtttaaag gttcttcgct gatgttcctc
    34981 aaaccgggca tgcaggttcc ggtttctcag ctgatccgcg gtattaacct gcaatcgggt
    35041 aacgatgctt gtgtcgccat ggccgatttt gccgctggta gccaggacgc ttttgttggc
    35101 ttgatgaaca gctacgttaa cgcactggga ctgaaaaata cccacttcca gacggtacat
    35161 ggtctggatg ctgatggtca gtacagctcc gcgcgagata tggcgctgat cggccaggcg
    35221 ttgatccgtg acgtaccgaa tgaatactcg atctataaag aaaaagaatt tacgtttaac
    35281 ggtattcgcc agctgaaccg taacggtctg ttatgggata acagcctgaa tgtcgacggc
    35341 atcaaaaccg gacacactga caaagcaggt tacaaccttg ttgcttctgc gactgaaggc
    35401 cagatgcgct tgatctctgc ggtgatgggc ggacgtactt ttaaaggccg tgaagccgaa
    35461 agtaaaaaac tgctgacctg gggcttccgt ttcttcgaaa ccgttaaccc actgaaagta
    35521 ggtaaagagt tcgcctctga accggtttgg tttggtgatt ctgatcgcgc ttcgttaggt
    35581 gttgataaag acgtgtatct gaccattccg cgtggccgca tgaaagatct gaaagccagc
    35641 tatgtgctga acagcagtga attgcatgcg ccgctgcaaa agaatcaggt cgtcggtact
    35701 atcaacttcc agcttgatgg caaaacgatc gaacaacgcc cgctggttgt gctgcaagaa
    35761 atcccggaag gcaacttctt cggcaaaatc attgattaca ttaaattaat gttccatcac
    35821 tggtttggtt aaaaatcgaa cacttgaaag tgtaatttcc gtccccatat actaagcatc
    35881 agtaaaaaac tcccgccttc tggcgggagt tgctatttaa ttacgttacg ccggagctga
    35941 catgaaaacc aaacttaacg aactgcttga attccctact ccttttactt acaaagttat
    36001 ggggcaggcg ttacctgagc tggttgatca ggtggttgaa gtggtacagc gccatgcgcc
    36061 aggtgactac accccaacgg taaaaccaag cagcaaaggc aactaccact cggtatctat
    36121 cactatcaac gctactcata tcgagcaggt tgaaacactg tatgaagaac tgggcaaaat
    36181 cgatattgtc cgcatggttc tgtaactcgc ttctccgtta cccggctcct ggtcgggtaa
    36241 ctcccctctc ctcgctgtga tatacttttc ccccactttt actcattctc cacggagatg
    36301 ccgttttgta tcaggataaa attcttgtcc gccagctcgg tcttcagcct tacgagccaa
    36361 tctcccaggc tatgcatgaa ttcaccgata cccgcgatga aagtaccctt gatgaaatct
    36421 ggctggtcga gcactatccg gtatttaccc aagggcaggc aggaaaagcg gagcacattt
    36481 taatgccggg tgatattccg gtgattcaga gcgatcgcgg tgggcaggtg acttatcacg
    36541 ggccggggca acaggtgatg tatgtgttgc ttaacctgaa acgccgtaaa ctcggtgtgc
    36601 gtgaactggt gaccttgctt gagcaaacag tggtgaatac cctggctgaa ctgggtatag
    36661 aagcgcatcc tcgggctgac gcgcctggtg tctatgtcgg ggaaaagaaa atttgctcac
    36721 tgggtttacg tattcgacgc ggttgttcat tccacggtct ggcattaaac gtcaatatgg
    36781 atctttcacc atttttacgt attaatcctt gtgggtatgc cggaatggaa atggctaaaa
    36841 tatcacaatg gaaacccgaa gcgacgacta ataatattgc accgcgttta ctggaaaata
    36901 ttttagcgct actaaacaat cctgacttcg aatatattac cgcttaattg catacatcaa
    36961 tggcccaatt tacactgggt cattactcat aatacctgct aatgctttac atctccttcg
    37021 cgtttgtata ttttctttat cacttgcaga aatgcgatgc tacgccttgc tccgtaactc
    37081 accccattga ttaacttatt gaaatagttg attatggtga gcaaaagcgt tccgttccaa
    37141 ttagacataa gattattgta atttttttta atatta
//
LOCUS       DACBVI010000029        37120 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.029, whole genome shotgun sequence.
ACCESSION   DACBVI010000029 DACBVI010000000
VERSION     DACBVI010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.029"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            208..1866
                     /locus_tag="IU325_07275"
     CDS             208..1866
                     /locus_tag="IU325_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2813 domain-containing protein"
                     /protein_id="HAP5808836.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1863..2819)
                     /locus_tag="IU325_07280"
     CDS             complement(1863..2819)
                     /locus_tag="IU325_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HAP5808837.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            2970..4085
                     /gene="macA"
                     /locus_tag="IU325_07285"
     CDS             2970..4085
                     /gene="macA"
                     /locus_tag="IU325_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HAP5808838.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKSGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4082..6028
                     /gene="macB"
                     /locus_tag="IU325_07290"
     CDS             4082..6028
                     /gene="macB"
                     /locus_tag="IU325_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HAP5808839.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAVEKVNVAGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6101..6325)
                     /gene="cspD"
                     /locus_tag="IU325_07295"
     CDS             complement(6101..6325)
                     /gene="cspD"
                     /locus_tag="IU325_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HAP5808840.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6648..6968
                     /gene="clpS"
                     /locus_tag="IU325_07300"
     CDS             6648..6968
                     /gene="clpS"
                     /locus_tag="IU325_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HAP5808841.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            6999..9275
                     /gene="clpA"
                     /locus_tag="IU325_07305"
     CDS             6999..9275
                     /gene="clpA"
                     /locus_tag="IU325_07305"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HAP5808842.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9618..9752)
                     /locus_tag="IU325_07310"
     ncRNA           complement(9618..9752)
                     /ncRNA_class="other"
                     /locus_tag="IU325_07310"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(9797..9884)
                     /locus_tag="IU325_07315"
     tRNA            complement(9797..9884)
                     /locus_tag="IU325_07315"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9848..9850),aa:Ser,seq:gga)
     gene            complement(10138..10356)
                     /gene="infA"
                     /locus_tag="IU325_07320"
     CDS             complement(10138..10356)
                     /gene="infA"
                     /locus_tag="IU325_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP5808843.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10641..11345)
                     /locus_tag="IU325_07325"
     CDS             complement(10641..11345)
                     /locus_tag="IU325_07325"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HAP5808844.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11387..13108)
                     /gene="cydC"
                     /locus_tag="IU325_07330"
     CDS             complement(11387..13108)
                     /gene="cydC"
                     /locus_tag="IU325_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HAP5808845.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNGSPIASLNEATLRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMHEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13109..14875)
                     /gene="cydD"
                     /locus_tag="IU325_07335"
     CDS             complement(13109..14875)
                     /gene="cydD"
                     /locus_tag="IU325_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HAP5808846.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEELFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQTALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFSTLLAHRQEEI"
     gene            complement(14998..15963)
                     /gene="trxB"
                     /locus_tag="IU325_07340"
     CDS             complement(14998..15963)
                     /gene="trxB"
                     /locus_tag="IU325_07340"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP5808847.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16508..17002
                     /gene="lrp"
                     /locus_tag="IU325_07345"
     CDS             16508..17002
                     /gene="lrp"
                     /locus_tag="IU325_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HAP5808848.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17137..21204
                     /gene="ftsK"
                     /locus_tag="IU325_07350"
     CDS             17137..21204
                     /gene="ftsK"
                     /locus_tag="IU325_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP5808849.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPAQPTVAWQPVPGPQTGEPVIAPAQEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYATAPEQSAQQSYYAPA
                     PEQSVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPVAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKTPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSAGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFTLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPIAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQQQYQQPQQPVAQQPQYQQPQQPVAPQPHDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            21363..21974
                     /gene="lolA"
                     /locus_tag="IU325_07355"
     CDS             21363..21974
                     /gene="lolA"
                     /locus_tag="IU325_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HAP5808850.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGTVDAAKFTFTPPQGVTVDDQRK"
     gene            21985..23328
                     /locus_tag="IU325_07360"
     CDS             21985..23328
                     /locus_tag="IU325_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP5808851.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23419..24711
                     /gene="serS"
                     /locus_tag="IU325_07365"
     CDS             23419..24711
                     /gene="serS"
                     /locus_tag="IU325_07365"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP5808852.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24950..27394
                     /gene="dmsA"
                     /locus_tag="IU325_07370"
     CDS             24950..27394
                     /gene="dmsA"
                     /locus_tag="IU325_07370"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HAP5808853.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            27405..28022
                     /gene="dmsB"
                     /locus_tag="IU325_07375"
     CDS             27405..28022
                     /gene="dmsB"
                     /locus_tag="IU325_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HAP5808854.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28024..28887
                     /locus_tag="IU325_07380"
     CDS             28024..28887
                     /locus_tag="IU325_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HAP5808855.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(28922..29548)
                     /locus_tag="IU325_07385"
     CDS             complement(28922..29548)
                     /locus_tag="IU325_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HAP5808856.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29863..31011
                     /locus_tag="IU325_07390"
     CDS             29863..31011
                     /locus_tag="IU325_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5808857.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASICFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            31221..32651
                     /locus_tag="IU325_07395"
     CDS             31221..32651
                     /locus_tag="IU325_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP5808858.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(32652..32762)
                     /locus_tag="IU325_07400"
                     /pseudo
     CDS             complement(32652..32762)
                     /locus_tag="IU325_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(32861..33601)
                     /gene="pflA"
                     /locus_tag="IU325_07405"
     CDS             complement(32861..33601)
                     /gene="pflA"
                     /locus_tag="IU325_07405"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HAP5808859.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(33793..36075)
                     /gene="pflB"
                     /locus_tag="IU325_07410"
     CDS             complement(33793..36075)
                     /gene="pflB"
                     /locus_tag="IU325_07410"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP5808860.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(36130..36987)
                     /gene="focA"
                     /locus_tag="IU325_07415"
     CDS             complement(36130..36987)
                     /gene="focA"
                     /locus_tag="IU325_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HAP5808861.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         9104 a         9059 c         9912 g         9045 t
ORIGIN      
        1 ctttaggcgt aataaaaaat aatttatcat gctaattatt tgattttgtt gtttttgcag
       61 gcttatcagc aagagggagt ataacgcgat tattcgctca tttttcagac atttgccatg
      121 cttaaatgtg atgtcatcac gtattagcat ggcctttccc gttatactgc cagcgtaaag
      181 gataagtcac atatttctgg aggggatatg attcttgagc gcgttgaaat tgtgggtttt
      241 cgcggtatca accgtttgtc gttgatgctg gaacaaaaca acgtcctgat tggggagaac
      301 gcgtggggta aatccagctt gctggacgcc ttaaccctgc tgctatcgcc agaatcagat
      361 ctctaccatt ttgagcgcga cgatttctgg ttcccgccgg gagatatcaa cgggcgagaa
      421 catcatctgc atattatttt gaccttccgc gaatcgctgc caggccgaca tcgggttcgc
      481 cgctatcggc cgctggaagc gtgctggacg ccatgcaccg atggctatca ccgtattttt
      541 tatcgtctgg aaggggagag tgcggaagac ggcagcgtga tgacactgcg cagttttctc
      601 gataaagacg gacatccgat tgatgtcgag gatattaacg accaggcacg ccatctggtg
      661 cgtttaatgc cggtgctgcg cttgcgtgat gcccgtttta tgcgccgtat tcgtaacggc
      721 acggtgccaa atgtccctaa tgtggaagtc accgcgcgcc agctcgattt cctcgcccgt
      781 gagttatcct cacatccgca aaatctctct gatgggcaga ttcgtcaggg actttccgca
      841 atggtgcaac tgcttgagca ttatttctct gagcaggggg ccggacaggc gcgatatcgt
      901 ttaatgcggc ggcgggccag caatgagcaa cgaagctggc gctatctgga tatcatcaac
      961 cggatgattg accgacctgg tgggcgctcg tatcgggtta ttttgctcgg cctgtttgct
     1021 actttattgc aggcaaaagg cacattgcga ctggataaag acgcccgtcc attgttgctg
     1081 atcgaagatc cagaaacccg tttacacccc attatgcttt cagttgcctg gcatctgttg
     1141 aatcttctgc cattgcagcg cattgccacc accaactcgg gtgagttgct ttcgttaacg
     1201 ccggtagagc atgtttgccg actggtacgt gagtcctcgc gcgttgccgc ctggcgtctg
     1261 gggccgagtg gcttgagtac cgaagatagc cgacgcatat cctttcacat tcgttttaac
     1321 cgtccgtcat cgctgtttgc acgctgctgg ctgctggtgg aaggggaaac ggaaacttgg
     1381 gttatcaatg aactggcgcg tcagtgcgga catcattttg atgccgaagg gatcaaggtc
     1441 attgagtttg cccagtccgg gctaaagcca ctggttaaat ttgcccggcg aatggggatt
     1501 gaatggcatg tactggtcga tggcgatgaa gcagggaaga aatatgccgc tacggtacgc
     1561 agcctgttga ataacgatcg ggaagccgaa cgagaacatt taacggcgtt accggcgctg
     1621 gatatggaac attttatgta tcgccaggga ttttccgatg tgttccaccg cgtggcgcaa
     1681 atcccggaaa atgtaccgat gaatctgcgc aaaattatct cgaaagcgat ccatcgctct
     1741 tccaaacccg atcttgccat tgaagtggca atggaggcag gacgtcgtgg tgtggattcc
     1801 gtaccgacgc tgctgaaaaa aatgttctca cgcgtgctgt ggctggcgcg cggtcgcgcg
     1861 gattaaccgc gaaacatcgt ggccatttgt ggctgaatag cgtcgagcat ctcatagcgc
     1921 cgacggtatt cagcccgttt tttactggcg atttcggcaa tctcttttcg tgctatctgt
     1981 gccggaaggc ggtaatggcg ttcagcatca catacgccgc caaccgactc ccagaaagcg
     2041 ttgtaatcgg catggatctt gccttcttta tcgcgataac gcaggctgcg gtaaatatgc
     2101 gtttcattgc tgacggcaat aatctgctct acctgcaaac gttgggcaaa cagacaggct
     2161 gcttccatca caaggcgttt gggaaatagc ccgtggcagg ctttcgtcgc attctggatt
     2221 tcctgatgtg gaatttccca ttttgcgcct tgcagtccgc caataaacat tgttcttttc
     2281 ccctgatatt cacacagggt aaacgtgatc tctgccagag gaataccttc gctgttgcgg
     2341 aacaggattg tgctgtcacc ttctttatcc attgagatca tcatggtcag ctcaagtgtg
     2401 aactgctcgc cgtttttgcc ttccagcttc gccagttgca gcccgggggt attcaaatat
     2461 aagctgaatt cttccgccga catacacccg cggagtaacg cataatggta acgtaacgcc
     2521 tccagcaatt gcttacggct aagattcgcc gcaaggtaag ggcgatgcag acgcacaggc
     2581 agtcgcggct ggcgcgttaa caacacattg agattaggcc agtgggaaag ttcgttcatc
     2641 cactcaacgc ttaaacgcgg cataatcaac gagcgcagca aaaatttctg gcgaaaacta
     2701 cggcgatgcc agaatttacc cggccgacac tgtccacgtg ccagactaag aaaaagtgac
     2761 aggctgctga gagattcaga tggcgtaaag gtccgttcag ttagctgcga catattcatg
     2821 aaatcaatgg ttatacatga cgtcgatttc accattgcgt atcttaacca aacatcaata
     2881 gtgtgattac taacgtaaat tttagggttt tgttgatatt tcgttgaagt taatgacccg
     2941 gattggcata tggagtattc agaatattta tgaaaaagcg gaaaaccgtg aagaagcgtt
     3001 acgttattgc gctggtgata gtcattgccg ggctgattac gttatggaga attcttaacg
     3061 cacccgtgcc gacttatcag acgctgattg tgcgccccgg tgatttacag caaagcgtgc
     3121 tggcgaccgg aaagctggac gcgctgcgta aggttgatgt gggcgcgcag gtcagcggtc
     3181 agttgaaaac gctgtcggtg gcgattggcg ataaagtaaa aaaagaccag cttttagggg
     3241 ttattgatcc tgaacaggct gaaaaccaga ttaaggaggt ggaagcaacg ctgatggagc
     3301 tacgcgcgca gcggcagcag gcggaagcgg agctgaaact ggcgcgggtg acgtattccc
     3361 gtcagcaacg tcttgcacaa acgcaagcgg tttcacagca ggatcttgat accgccgcga
     3421 cggagatggc tgtgaaacag gcgcaaattg gcaccattga cgcgcaaatc aagcgcaatc
     3481 aggcctcact cgatacggct aaaaccaatc tcgattacac ccgcatcgtt gccccgatgg
     3541 ccggggaagt cacgcaaatc accactctgc aaggccagac ggtgattgcc gcacaacaag
     3601 caccgaacat tctgacgctg gcagatatga gcaccatgct ggtaaaagcg caggtttctg
     3661 aagcggatgt aatccacctg aagtcggggc aaaaagcctg gtttacggtg cttggcgatc
     3721 cgctgacgcg ctacgagggg caaatcaagg atgtgctacc gacgccggaa aaggttaacg
     3781 acgctatttt ctattacgcc cgttttgaag tccccaaccc caatggtttg ctgcggctgg
     3841 atatgaccgc gcaagtgcat attcagctca ccgatgtgaa aaatgtgctg acgatccctc
     3901 tgtcggcgtt aggcgatccg gttggcgata atcgttataa agtcaaattg ttgcgtaatg
     3961 gtgaaacacg cgagcgtgaa gtgacgattg gcgcacgtaa cgataccgat gttgagattg
     4021 tcaaagggct ggaagcgggc gatgaagtgg tgattggtga ggccaaacca ggagctgcac
     4081 aatgacgccc ttgctcgaat taaaggatat tcgtcgcagc tatcctgccg gtgatgagca
     4141 ggttgaggtg ctgaagggca tcaccctcga tatttatgct ggtgagatgg tggcgattgt
     4201 cggcgcttcg ggttccggta aatcgaccct gatgaatatt ctcggctgcc tggataaggc
     4261 caccagcggc acctatcgcg tagccggtca ggatgttgcc acgctggacg ccgatgcgct
     4321 ggcgcaactg cgtcgcgaac acttcggctt tattttccag cgttaccatt tgctttcgca
     4381 tttaaccgcc gagcagaacg ttgaagtacc cgccgtctat gccggtcttg agcggaaaca
     4441 gcggctgctt cgcgcccagg agttactgca acgactgggg ctggaagacc gtacagagta
     4501 ttacccggca cagctttcgg gggggcagca acagcgcgtc agcatcgcgc gggcattgat
     4561 gaacggcggt caggtaattc ttgccgatga accaaccggc gcactggaca gccattctgg
     4621 cgaagaggtg atggcgatcc tgcatcagct gcgcgatcgc gggcatacgg tgattatcgt
     4681 tacccacgat ccgcaggtcg ctgctcaggc cgagcgggtg atcgaaattc gcgacggcga
     4741 aattgtgcgc aatcctcccg ccgttgaaaa agtgaatgtt gctggcggga cggaacctgt
     4801 tgtcaacacg gcctctggct ggcggcaatt tgtcagcggt tttaacgaag cgctgacgat
     4861 ggcatggcgg gcgctggcag cgaataaaat gcgtacttta ctgaccatgc tggggattat
     4921 tatcggtatt gcgtcggtgg tttcaattgt cgtggtgggc gacgccgcca aacaaatggt
     4981 gctggcggat attcgttcta ttggtacgaa tactattgat gtctatcccg ggaaagattt
     5041 tggcgatgac gatccgcaat atcagcaggc gctgaagtac gacgacttaa tcgccatcca
     5101 aaaacaaccg tgggtcgcct cagccacacc tgccgtctcg caaaacctgc gcctgcgtta
     5161 taacaatgtt gatgttgctg ccagtgccaa tggcgtgagc ggcgattatt ttaatgtcta
     5221 tggcatgacc ttcagtgaag ggaacacctt taatcaggag cagctgaacg gtcgtgcgca
     5281 ggtggtggtg ctcgacagta atactcgccg ccagcttttc ccccataaag cagatgtggt
     5341 tggcgaggtg attctggtcg gcaatatgcc cgccagagtc attggtgtgg cggaagaaaa
     5401 acagtcgatg tttggtagca gtaaagtgct gcgtgtctgg ctaccttaca gcacgatgtc
     5461 cgggcgagtt atgggccagt cgtggctgaa ctccattact gtcagggtga aagaaggatt
     5521 tgacagcgcc gaggcggaac agcaactcac gcgtttactt tcactgcgcc acggtaagaa
     5581 ggatttcttt acctggaaca tggacggcgt cttgaaaacc gttgaaaaga ccacacgtac
     5641 tttacaactg tttctgacgc tggtggcggt gatttcgcta gtggtgggcg gtattggtgt
     5701 aatgaatatt atgctggtgt cagtgaccga gcggacgcgg gaaattggca ttcgcatggc
     5761 tgtaggtgcg cgagcaagtg atgtgttgca acagttcctg atcgaagcag tactggtttg
     5821 cctggtcggt ggcgcgttgg gaataacact gtcactgtta attgctttca ccttacagct
     5881 tttcttaccc ggctgggaga ttggtttttc accgttggcg ctgctgctgg cgtttctctg
     5941 ctcgacggtc accgggattt tatttggctg gttacccgca cgaaatgcgg cacgactgga
     6001 tccagtagat gctctggcac gagagtaatt tttgagataa aaatgccagc cgatcgggct
     6061 ggcattttgc ctttaggatg tacacaatga gacagaagag ctatgcgact gccgcttcta
     6121 cttcgacggg cacaataaca ctggcgtgat tgccttttgg cccctggtgg acatcaaact
     6181 gaacggattg tccagctttt agcgttctgt aaccatccat ctgaatggtg gaataatgag
     6241 cgaaaatatc ttcgccgccg ccttcagggc agatgaaacc aaaccctttg gcattgttga
     6301 accacttaac agtacccttt tccatgcttc gacatccttc gcaaatctta tacaagtaag
     6361 atggaataaa ccggggtcag agagggggct gttcaaaacc tcgccaactc tagaaataca
     6421 atttagagaa ttagggcgag ccgtcaagca tttgacaggg gccagggggc aggtatgaat
     6481 caaaaatttg aagcagttaa cgctattgac aggaatgtga cagatgtcgc tgatgccaac
     6541 gatagatgat agttatctat catgtggagt agattggtca ggcaaataag ctcttgtcag
     6601 cggcagggcg ttctgccgat aaccgtaacc gaagatgata actgacaatg ggtaaaacga
     6661 acgactggct ggactttgat caactggcgg aagaaaaagt tcgcgacgcg ctaaaaccgc
     6721 catctatgta taaagtgata ttagtcaatg atgattacac tccgatggag tttgttattg
     6781 acgtgttaca aaaattcttt tcttatgatg tagaacgtgc aacgcaattg atgctcgctg
     6841 ttcactacca ggggaaggcc atttgcggag tctttaccgc cgaggttgca gaaaccaaag
     6901 tggcgatggt gaacaagtac gcgagggaga atgagcatcc attgctgtgt acgctagaaa
     6961 aagcctgaat gcaggcataa aaattggggg aggtgcctat gctcaatcaa gaactggaac
     7021 tcagtttaaa tatggctttc gccagagcgc gcgagcaccg tcatgagttt atgaccgtcg
     7081 agcacttgtt actggcgctg ctcagtaacc catctgcccg ggaggcgctg gaagcgtgtt
     7141 ctgtggattt ggttgcgctc cgtcaggaac tggaagcctt tattgaacaa accacacccg
     7201 ttctgcctgc cagtgaagag gagcgcgaca cacagccgac gctgagtttt cagcgtgtgc
     7261 tgcaacgtgc ggtcttccat gtccagtcct ccggtcgcaa tgaggttacc ggtgcaaacg
     7321 ttctggtcgc tatctttagc gaacaggagt cgcaggcggc atatctgttg cgtaaacatg
     7381 aagtcagccg tctcgatgtg gtgaacttta tctctcatgg cacgcgtaaa gacgagccga
     7441 cacagtcttc tgatcctggc agccagccaa acagcgaaga acaagctggt ggggaggaac
     7501 gtatggagaa tttcacgacg aacctgaatc agcttgcgcg cgtgggcgga atcgacccac
     7561 tgattggtcg tgagaaggag ctggagcgtg ctattcaggt tctctgccgt cgccgtaaaa
     7621 acaacccgct gctggtgggg gaatctggtg tcggtaaaac cgcgattgcg gaaggtcttg
     7681 cctggcgaat tgttcagggt gatgtgccgg aagtgatggc tgactgtacg atttactctc
     7741 tcgatatcgg ttctctgtta gcgggcacaa aatatcgcgg cgactttgaa aaacgtttta
     7801 aagcgttgct caagcagctg gagcaggaca ctaacagcat cctgtttatt gatgagatcc
     7861 acaccattat cggtgcgggt gcagcgtctg gtggtcaggt cgatgcggct aacctgatca
     7921 aaccgttgct ctccagcggt aaaattcgcg taattggttc gacaacctat caggagttca
     7981 gcaacatttt cgagaaagac cgtgctttgg cgcgtcgctt ccagaaaatt gatattactg
     8041 aaccgtcgat cgaagaaact gttcaaatca tcaacggcct gaaaccgaag tatgaagcgc
     8101 accacgatgt gcgttatacc gcaaaagcgg tgcgtgcagc ggtagagctg gcggtgaaat
     8161 acattaacga tcgtcatctg ccggataaag ccattgacgt catcgacgaa gcgggcgctc
     8221 gcgcacgcct gatgccggta agcaaacgca agaaaaccgt taatgtggcg gatattgagt
     8281 ccgtggtggc ccgtattgca cgcattccag agaagagtgt ttctcagagt gaccgcgata
     8341 ccctgaaaaa cctcggcgat cgcctgaaaa tgctggtctt cggtcaggat aaagccattg
     8401 aggcgctgac tgaagccatt aagatggcgc gtgcaggttt aggtcacgaa cataaaccgg
     8461 tcggttcgtt cctgtttgcc ggccctaccg gggtcgggaa aacagaggtg acggtacagc
     8521 tttcgaaagc tttgggcatt gagcttctgc gctttgatat gtccgagtat atggaacgcc
     8581 ataccgtcag ccgtctgatt ggtgcgcctc cgggatacgt tggttttgat cagggcggtt
     8641 tgctgactga tgcggtcatc aagcatccac atgcggtgct gctgctggac gaaatcgaga
     8701 aagcgcaccc ggacgtgttc aatattctgt tgcaggtgat ggacaacggt acgctgaccg
     8761 ataacaacgg acgcaaagcg gacttccgta acgtggtatt ggtgatgacc accaacgctg
     8821 gggtacgaga aactgagcgc aaatcgattg gtcttatcca ccaggacaac agcaccgatg
     8881 cgatggaaga gatcaagaag atctttacgc cggagtttcg taaccgtctc gacaacatta
     8941 tctggttcga tcatctctcc accgacgtga tccatcaggt agtggataaa ttcatcgtcg
     9001 agttgcaggt tcagttggat cagaaaggtg tttctctgga agtgagccag gaagcgcgta
     9061 actggctggc cgagaaaggt tacgaccggg caatgggcgc acgtccgatg gcgcgtgtca
     9121 tccaggacaa cctgaaaaaa ccgctcgcca acgaactgtt gtttggttcg ctggtggacg
     9181 gcggtcaggt gacggttgcg ctggataaag agaaaaatga gctgacttat ggattccaga
     9241 gtgcacaaaa gcacaaggcg gaagcagcgc attaatctga ttgtcaggta ggttggtgaa
     9301 gtccgtaatc tcgaaagagg ttacggactt tttgtttgta ggctggggcg gtgaaaaccc
     9361 tatttttgga ggtgaaggta agttgttgat aattaatggt gctggaaggt aagaataaaa
     9421 aagggtggca gcaggaaaat gagatggttt tgctttatta ataacggact aaacgtgtag
     9481 tatttgagtt cactgccgta caggcagctt agaaagccaa ccgaaagaaa aactcgattt
     9541 ttataccgtt cactgccgta caggcagata aaatgcgaaa aaaaactcgt actttcgtac
     9601 gagcttttct ttaaatatgg cggtgagggg ggattgactc gcttcgctcg ccctgcgggc
     9661 agcccactca ctgtgttcgt ggtctgtcca actggctgcg ccagttgtcg aaccccggtc
     9721 ggggcttctc atcccccctg gagtgcaata tgcgaaaaaa aaagctcgca ctttcgtacg
     9781 agcttttctt taaatatggc ggtgaggggg ggattcgaac ccccgatacg ttgccgtata
     9841 cacactttcc aggcgtgctc cttcagccac tcggacacct caccaaattg ttttgctacc
     9901 aaacctcatg ggtggcaacg gggcgctact atagggagtt ggagtaaaac ggtcaagaag
     9961 aattttaatg ataattattg tttgctcata ctgtaaacaa gttgtgcagt atatctacat
    10021 cgagacaagt tatggactta tacttccaaa gtacttcata catatcacaa aataaaaagg
    10081 ccggttaaac cgacctttta ctcgttcttt ctcttcgccc atcaggcggt aaaacaatca
    10141 gcgactacgg aagacaatgc ggcctttgct caggtcgtac ggggtcagtt caacagtcac
    10201 tttgtcgccc gtcaggatgc ggatgtagtt tttgcgcatt ttaccggaga tgtgtgcagt
    10261 aaccacgtga ccgttttcta actctacgcg gaacatggta ttaggcaacg tttcaagaac
    10321 ggtaccttgc atttcaatat tgtcttcttt ggccatctaa tcctctgggg tatcactacc
    10381 gtaatttgaa ccggcaagat aatgccgaag ttctgtaaat aagtaaagat ttgcgcgcta
    10441 aatcgcaaca aacaggttcg gcacattact ccgaaaacac acggctaagc cgcaccaaaa
    10501 gcgcaacgta taagggagcg gtgagataaa cgatgggcgt tacctgacgc gaaaaattcc
    10561 ttatcggcag cggggtaatg agcgtaacca actctgcgac cgcaattata acactctggg
    10621 gagaaatgtg ccgaaaacat tcattcttgt ggtgaaaaca agcaccgtgg tacccagaaa
    10681 ttattcggca atcgtccgag gcgcatttga ttgagataat tgaggtaatc ccggcgggga
    10741 atttcgcagg caccaagcga cgctgtgtga tcgttaagta cctggcagtc gataagctta
    10801 ccgccatgac caataaattc ctcacagaat accagaagcg ccgttttaga cgcattttcc
    10861 atccggctga acatggactc accacaaaat agcgttccct gggccacgcc gtacataccg
    10921 ccgacaagct catcttcacg ccagacttca atggagtggg catgaccgag ttcgtgaagg
    10981 cgatggtagg cttcgaccac gccacgcgtg atccaggttc cttcttcgcg atcgctggca
    11041 cagccttcaa tgacctgacc aaaagcgtaa ttcatcgtga cacgataggg cgagcgttta
    11101 tgaaatcgct tcatactacg gctgatatgc agtgattcag gccaaagcac cgcgcgggga
    11161 tcgggcgacc accagaggat ggggtcgccg ggagaaaacc acggaaaaat accacgctgg
    11221 taagccatta acaggcgtgc agggctaaga tcgcccccaa gtgccagcag gccgttaggc
    11281 tcacgtaatg cgccttccgg ggaagggaag gctattgaat ggcgagaaag ctgaaccagg
    11341 cgcatgaccg caaaactcca cgcaagtcgg atcgttcaat aatagcttac aaaccctgct
    11401 taaactggta ataacgcccc tgtctggcaa gcagttctgc gtgagtacct tgctcaataa
    11461 tttgcccgtt gtccatcact attatttgtt ggaaacgaga gagtccgcga agtcgatggg
    11521 tgaccattaa caccgttttc tcatgcatca tttctgcaag caattcaagg atctgacttt
    11581 cggttgtggc atctaagcct tctgtaggtt catccagcaa taccagcggc gcatcatgta
    11641 acagcgcacg ggcgatagcc agacggcgca gttcaccacc ggagagctgg cgtccgcctt
    11701 cacctaacca actgttgaga cctgcatcct cgagcagctt ctccagacca acgcgacgta
    11761 agatctccgc cagagcctca tcactactgc caggcgaggc gagtaacaga ttatcacgca
    11821 gcgtggcgct aaacagatgc actcgctgag gaacaacgct gatggtctgt cgtagagtcg
    11881 cttcattcag gctggctatg gggctaccgt taagcaaaat ctcgccctgt tgcgggtccc
    11941 atgcgcgggt cagcagttgt aacagtgttg atttgccgca tccggttcgc ccgagaatcg
    12001 ctatatgttc tccggcgttt acctgaagag aaatcccttt aagtgcctgt tgggattgct
    12061 ccggataagt gaactgaaca tcccgtaacg tcaacgaaac gcgatcggca acacgagttt
    12121 gggtatcagg gaaggtgact tccggttttt gatccgttaa gtcagtgatc cgaacggcag
    12181 aggcaatgac ttgccccaga tgctgaaatg cacccgttac tggtgccagt gcttcaaacg
    12241 cggctaacgc gcagaagaca aacagggcaa ttaacgcgcc gggttgagca ttgccgccaa
    12301 cgccgccaga cgccatccac agcatcagga tcaccgctaa cgcgccaatg agcagcatta
    12361 tcgcttgcga caatgcggtg agttcagatt gacgtcgttg cgcttccagc cactgaattt
    12421 cggtattctc taactgcgtg cgataacgat cgctggcacc aaaaatggtc agctcagctt
    12481 gcccttgcag ccaggccgtc agttgttggc gatactgccc gcgaagatga gtcagatttt
    12541 gcccggtgct ttttcccgca cgataaaaca gcggtggcat caggaaaagc gtcagtaaca
    12601 taatgccgcc cagcgtaaag gcgagggtga aatcaaggaa acttaacccg attgtcacca
    12661 ccataatcac cacaaaagcg cccaccagcg gcgagataac gcgcaggtaa agatgatcga
    12721 gcgtatcaac atccgccacc acgcggttga gcaattcgcc ctgacgatag cgcgccagtc
    12781 cggcagggga gaggggcagc aatttgctga aggtataaat gcgcagatgc tgcaacacgc
    12841 ggaaagtcgc gtcgtgactt accagacgtt caaaatagcg cccggcagta cgggtgattg
    12901 ctgcaccacg cacgcccgca gcgggtagca tatagttgaa gctgtacagt ccggcaaccc
    12961 ccgctaccgc tgaggccgag aggaaccagc cggaaagtgt caacagaccg atactggcga
    13021 gcagcgtgac aattgccagc acaataccaa gacttaacat ccatttatga cgtttataca
    13081 gtgccagata gggtagcaaa gcgcgcattt aaatctcctc ctgacgatgg gccagcaatg
    13141 tggagaaagg accaccagcc acgcttaatt ccgcgtaacg tccttgctca ataatctgac
    13201 catcctgcat tacccaaatg acatcccagt cagcaagatc ttctaactgg tgggtgacca
    13261 ttaacgttgt ctggcgcaga gaggcggcat taagcgcctc cattacgcgc tgttcactgt
    13321 gagcatcaag gctggcagcg ggttcatcca acagtaatag cgaacaggga tttagtaatg
    13381 ctcgggccac cgccacgcgc tgtgcctgcc ccacggaaag gcgggcagcc tggtcgccaa
    13441 caggcgtatc aacgccttgt ggcaggagcg gtagaaactc actgacccag gcgttatcca
    13501 gcgctgtttg taactcgtgt tcgctggcat caggtcgtgc cagtagtacg ttatcccgta
    13561 gtgttgctgc cggtaattgt gggttttgcc caacccagga gagatgttta cgccatgatt
    13621 ccggtgataa atcgcgtaat tctatcccgt tgattcgtag cgatccctga tatgagagaa
    13681 aaccagaaag cgcgttcagc agtgagcttt tacctgaacc gctgcgacca accaacacgg
    13741 cacgttggcc tgctggcaaa gtaaagttca gcggtccggc cagcgtttta ccttctggtg
    13801 atgtaataaa cagctcctcg gcttcaatgg tcaccggatc ggtcgatgct aattccgcct
    13861 caccacgctg cggatgggca agcggggttt ccataaacgt tttcaaactg tcagctgcac
    13921 caacagcctg ggctttagca tgataaaacg taccgagatc gcgtaatggc tggaaaaact
    13981 ccggcgcaag gatcagggcc agaaaacccg cagccagcgt cacgccagta tcatagtgac
    14041 caaaatccag ctcgccgaga taggaaaaac caaagtagac cgccaccaga gcaattgaca
    14101 gcgaggtaaa aaattcgaga atgccggagg ataaaaacgc cagccgtagc acttccattg
    14161 tccgttggcg gaaatcttcc gaagcagaac gaatactttc aatttcagct tcaccacgac
    14221 caaaaatacg caatgtttcc atgccgcgca ggcgatcgag aaaatgccca cttaagcgag
    14281 caagagcgag aaagttacgt cggttagcat cggcagcccc cattccaacc agcgccataa
    14341 acaacggaat taacggagca gtgcccagca gaatgagcgc cgcagcccag ttagagggga
    14401 agatagccac cacaatcagc aacggcaccg acactgccag cgccatttgc ggcaggtagc
    14461 gtgcatagta atcatgcata tcgtcaattt gctcgagtac cagcgtcgcc cagctccccg
    14521 ccggtttacc ctgaatccac gctggccctg cttgttgcag acggtcgaga acctgacggc
    14581 ggatggcaaa gcggatatgc tgcccggcgt gataacccac ccgttcgcgt aaccagacca
    14641 cccatgcgcg aagtacaaag gtcagaacca gtaacgtaaa gggaagcagc agggcttcac
    14701 ggggaatatt ctccataatc atatgttgca gaattcgcgc catgaaccag gcctgggcaa
    14761 tgatcaatat gccgctcaca aagcccagca gacgagaaat attcagccaa cgttgggaga
    14821 tgacgctttg ctgttttaac cagcgggtta actctttttg acgggattta ttcattgcac
    14881 gcttagcagg tgagttatca gaattatttg cagagcaatg ttacaacgag gaaaaaataa
    14941 aggcgaccca tagtcgcatg gtgtcgcctt ctttactttt gttactgatt tgtaaaatta
    15001 ttttgcgtca gctaaaccat cgaggtagcg ttccgcatca agtgctgcca tgcagcctgt
    15061 accagcagat gtaatagcct ggcgataaat gtgatccatc acgtcgcctg cggcaaagac
    15121 gccagggatg ctggtctggg tggcattacc atgaataccc gactgtactt tgatgtagcc
    15181 gttttccagt tccagctgcc cttcgaaaat ggcagtattc gggctgtgac cgatagcaac
    15241 aaacagaccg gcaacgtcga gtgactcgat gttatcgctg ttttgcgtat cgcgcagacg
    15301 aacgccagtg acgcccatct gatcgccggt cacctcttcc agcgtacggt tggtgtgtag
    15361 aatgatgttg ccgttctcca ctttatccat cagacgctta ataaggattt tttccgcgcg
    15421 gaaaccgtca cggcggtgaa tcagatgaac ttccgaagcg atgttagaca gatacagcgc
    15481 ctcttcaacc gcggtattgc cgccgccgat gaccgcaact ttctggttgc gatagaagaa
    15541 accgtcgcag gttgcacaag cagaaacccc acggccttta aacgcttcct cagaaggaag
    15601 gccgagatag cgtgcagaag ctccggtggc aataatcagc gcgtcgcaag tgtattcgcc
    15661 gttatcgcca ttcagacgga acggacggtt ttgcagatcc accttgttga tatgatcaaa
    15721 aatgatctca gtttcaaatt tggtggcatg ttcgtgcatg cgctccatta ataacggacc
    15781 ggtcagatcg tttggatcgc caggccagtt ttccacttcc gtggtggtgg tcagttggcc
    15841 gcctttttcc atgccggtaa tcagcacagg ttgcaggttg gcgcgcgccg catagacagc
    15901 agcggtgtat cccgccgggc ctgaacccag gataagcagt ttactgtgtt tggtcgtgcc
    15961 catgagatcc ccatagttgt tggcagacaa tgggcaggat tgtagggaat ttacagacgt
    16021 aaaaaaagag tatgacgatt ttgttaacaa tttgtgcaat cggcagcatc gataagcagg
    16081 tcaaattctc ccgtcattat cacctctgct acttaaattt cccgctttat aagccgatta
    16141 aatgatgaat aaacgcccct agtaatgaat atctggcatg ttgtactaaa aatcgatgtt
    16201 ttgctttgac aatcccctgg tgttttgcga aaacattcga ggaagaaaaa aaacagtatt
    16261 cttatatgcg cataaccatg catgtaaata ccatgtttac cgtgctagtg aaatctacgt
    16321 atggcgtgga cagacgccat tcgtgatgtc gatagcagcc acaaggcaac ggtcttctca
    16381 ccgtagaccc aggcattgcg cgccgtgaat cttcatgatt tcggtctatc gtgacgggta
    16441 gcgactctga acagtgatgt ttcagggtca gacaggagta gggaaggaat acagagagac
    16501 aataataatg gtagatagca agaagcgccc tggcaaagat ctcgaccgta tcgatcgtaa
    16561 cattcttaat gagttgcaaa aggatgggcg tatttctaac gtcgagcttt ctaaacgtgt
    16621 gggactttcc ccaacgccgt gccttgagcg tgtgcgtcgg ctggaaagac aagggtttat
    16681 tcagggctat acggcgctgc ttaaccccca ttatctggat gcatcacttc tggtattcgt
    16741 tgagattact ctgaatcgtg gcgcaccgga tgtgtttgaa caattcaata ccgctgtaca
    16801 aaaacttgaa gaaattcagg agtgtcattt agtatctggt gatttcgact acctgttgaa
    16861 aacacgcgtg ccggatatgt cagcttaccg taagttgctg ggggaaaccc tgctgcgtct
    16921 gcctggcgtc aatgacacac ggacatacgt tgttatggaa gaagtcaagc agagtaatcg
    16981 tctggttatt aagacgcgct aacacggaac aggtgcaaaa tcggcgtatt ttgattacac
    17041 tcctgttaat ccatacagca acagtactgg ggtaacctgg tactgttgtc cgttttagca
    17101 tcgggcagga aaagcctgta acctggagag cctttcttga gccaggaata cactgaagac
    17161 aaagaagtca cattgacaaa gttaagtagc ggccgccgcc ttctggaggc gttgctgatc
    17221 cttattgtcc tgtttgccgt ctggttgatg gctgccttac taagctttaa cccttcggac
    17281 cccagctggt cgcaaacggc ctggcatgaa cctatccata atttaggtgg gatgcccggt
    17341 gcatggctgg cagatacgct gttctttatt tttggcgtga tggcttacac cattcccgtc
    17401 attattgtcg gcggttgttg gtttgcctgg cgtcatcagt ccagcgacga atacattgat
    17461 tattttgccg tttcgctacg catcattggc gttttggcgc tcatccttac ctcctgtggt
    17521 ctggcggcaa tcaacgctga cgatatctgg tattttgcct ccggtggcgt cattggcagc
    17581 ttactaagca ctacgctaca accactgcta cacagtagcg ggggaactat tgcgctgctc
    17641 tgcgtttggg ctgcgggtct gacgctgttc accggttggt catgggtgac cattgctgaa
    17701 aaactcggcg gctggatttt aaacattctc accttcgcca gtaatcgtac ccgtcgcgat
    17761 gatacctggg tcgatgaaga tgagtatgaa gacgacgaag agtatgaaga tgaaaatcac
    17821 ggcaaacagc atgaatcacg ccgtgcccgt attcttcgcg gcgcgttagc gcgtcgtaaa
    17881 cggttggcgg aaaaattcat taatccgatg gggcggcaaa cagatgctgc gttgttctcc
    17941 ggtaagcgga tggatgatga agaagagatt acctacactg cacgcggtgt ggctgccgac
    18001 ccggacgacg tcctcttttc gggcaatcgt gcaacgcagc cagaatatga cgaatacgat
    18061 ccattattaa acggtgcgcc aattaccgaa cctgttgctg tagcagctgc tgctaccacg
    18121 gcgacacaaa gctgggctgc gccggttgaa cctgtgactc agacgccgcc tgttgcctct
    18181 gttgatgttc cacctgcaca acctacagta gcctggcagc ctgtaccggg gccacaaacg
    18241 ggagagccgg ttattgctcc tgcacaggaa ggttacccac agcagccaca atatgcgcag
    18301 cctgcagtgc aatataatga gccgctgcaa caacctgtac agccacagca gccgtattat
    18361 gcgccagcag cggaacaacc tgtgcaacaa ccttactacg ctcctgcggc ggaacaacct
    18421 gttcagcagc cgtattacgc aactgcgccg gaacagtctg cgcaacagtc gtattacgcc
    18481 cctgcgccag aacaatcggt ggcaggtaac gcctggcaag ccgaagagca gcaatccact
    18541 tttgctccac agtctacata ccagactgag caaacttatc agcagccagt cgctcaggag
    18601 ccgttgtacc aacagccgca acctgttgaa cagcagcctg ttgtggagcc tgaacccgtt
    18661 gtagaagaga caaaacccgc gcgtccgccg ctttactatt ttgaagaagt ggaagagaag
    18721 cgtgcccgtg aacgtgaaca acttgcggcc tggtatcaac cgattcctga accggttaaa
    18781 gaaccagaac cgatcaaatc ttcgctgaaa acaccttctg ttgcagcagt acctccagtg
    18841 gaagccgctg ccgctgtttc ccctctggca tctggcgtga aaaaagcgac actggcgacg
    18901 ggggctgccg caaccgttgc cgcgccagtc ttcagtctgg caaatagcgc tggaccgcgt
    18961 ccacaggtca aagaggggat tggcccgcag ttgccacgac cgaaacgtat ccgcgtgcca
    19021 actcgtcgtg aactggcgtc ttacggtatt aagctgccct cacagcgtgc ggcagaagaa
    19081 aaagcccgtg aagctcagcg caatcagtac gattccggcg atcagtataa cgatgatgaa
    19141 atcgatgcga tgcagcagga tgaactggca cgtcagttcg cccagacaca gcagcaacgc
    19201 tatggcgaac agtatcaaca tgatgtgccc gtgaacgcag aagatgcaga tgctgcggca
    19261 gaggctgaac tggctcgtca gtttgcgcaa actcaacaac aacgttattc cggcgaacaa
    19321 ccggctgggg cgaatccgtt cacgctggat gattttgaat tttcgccaat gaaagcgttg
    19381 ctggatgatg gtccacacga accgttgttt acgccaattg ttgaacctgt acagcagccg
    19441 caacaaccga ttgcaccgca gcaacagtat cagcagccgc agcaaccagt tgcgccgcag
    19501 ccgcagtatc agcagccgca acagccggtt gcaccgcagc agcaatatca gcagccacaa
    19561 caaccggttg cgccgcagca gcaatatcag cagccgcaac agccggttgc acagcagccg
    19621 caatatcagc agccgcaaca gccagttgcg ccgcagccgc atgataccct gcttcatccg
    19681 ctgttgatgc gcaatggtga cagccgtccg ttgcataaac cgacgacgcc gctgccttct
    19741 ctggatttgc tgacaccgcc gccgagcgaa gtggagccgg tagatacctt tgcgcttgaa
    19801 caaatggcgc gtctggtgga agcgcgtctg gctgatttcc gtattaaagc cgatgtcgtc
    19861 aattactctc cggggccggt tatcactcgc tttgaactta acctggcacc gggcgtaaaa
    19921 gcggcgcgca tttctaactt gtcacgggac cttgcccgtt cactttcgac ggtggcggtg
    19981 cgtgtcgttg aagttattcc tggcaaaccc tatgtaggtc tggagttacc gaataaaaaa
    20041 cgacaaaccg tttatctgcg cgaagtattg gataacgcca aattccgcga taatccgtcg
    20101 ccattaaccg tggtgctggg taaagatatc gccggtgagc cggtggttgc cgatctggcg
    20161 aaaatgccgc acttgttggt tgcggggact accggttccg gtaagtctgt cggggtgaac
    20221 gcgatgatcc tgagcatgct ttataaagca cagccagaag atgtgcgttt catcatgatc
    20281 gacccgaaaa tgctggagct ttcggtttat gaaggcattc cgcatctgtt aacggaagtc
    20341 gttaccgaca tgaaagatgc tgccaacgct ttgcgctggt gcgtaaatga aatggaacgt
    20401 cgttataagc tgatgtccgc cctaggtgtg cgtaatctgg cgggttataa cgaaaaaatt
    20461 gctgaagccg atcgcatgat gcgtccgatt ccagacccgt actggaagcc gggtgacagt
    20521 atggatgccc agcatccggt gctgaaaaaa gaaccgtaca ttgtggtgtt ggtggacgaa
    20581 tttgccgacc tgatgatgac ggtaggtaaa aaagtggaag agcttatagc acgtctggcg
    20641 caaaaagccc gtgccgcggg tatccacctc gtactggcaa cgcagcgtcc atcggttgat
    20701 gttattactg gtctgattaa agcgaatatt ccgacccgta tcgcctttac cgtatccagt
    20761 aagattgact cacgtaccat tcttgatcag gctggcgcgg aatcactgct gggtatgggg
    20821 gatatgcttt actctgggcc gaactccacg ttgccggtac gtgttcatgg tgcttttgtt
    20881 cgcgatcagg aagttcatgc cgtggtgcag gactggaaag cgcgtggtcg cccacagtat
    20941 gttgatggca tcacctccga cagtgaaagc gaaggtggcg cgggcggttt cgatggcgct
    21001 gaagagctgg atccgttgtt cgatcaggcg gtgcagtttg tcactgaaaa acgcaaagca
    21061 tcaatttctg gcgtacagcg tcagttccgc attggttata accgtgcagc gcgtattatc
    21121 gaacagatgg aagcgcaggg gattgtcagc gaacaggggc acaacggtaa tcgtgaagtg
    21181 ctggccccac cgccgtttga ctaattaatg catcgtaggc cggataaggc tcggtagcgt
    21241 cgcatccggc actctaacaa ctgaaaattc agtattttct tctttcctca agctgattat
    21301 tagcctggaa tagagagtag agggaactcc cgatcgggag tgacgtaatt tgaggaataa
    21361 tgatgaaaaa aattgccatc acctgtgcat tactctcaag cttagtagca agcagcgttt
    21421 gggctgatgc cgcaagcgat cttaaaagcc gcctggataa agtcagcagc ttccacgcca
    21481 gcttcacaca aaaagtgact gacggtagcg gcgcggcggt gcaggaaggc cagggcgatc
    21541 tgtgggtgaa acgtccaaac ttattcaact ggcatatgac tcaacctgat gaaagcattc
    21601 tggtttctga cggtaaaaca ctgtggttct ataacccgtt cgttgagcaa gctacggcaa
    21661 cctggctgaa agatgccacc ggtaatacgc cgtttatgct gattgcccgc aaccagtcca
    21721 gcgactggca gcagtacaat atcaaacaga atggcgatga ctttgtcctg acgccgaaag
    21781 ccagcaatgg caatctgaag cagttcacca ttaacgtggg acgtgatggc acaatccatc
    21841 agtttagcgc ggtggagcag gacgatcagc gcagcagtta tcagctgaaa tcccagcaaa
    21901 atgggactgt ggatgcagcg aaatttacct tcaccccgcc gcaaggcgta acggtagatg
    21961 atcaacgtaa gtagaggcac ctgagtgagc aatctgtcgc tcgatttttc ggataatact
    22021 tttcaacctc tggccgcgcg tatgcggcca gaaaatttag cacagtatat cggccagcaa
    22081 catttgttgg ctgcggggaa gccgttgccg cgtgctatcg aagccgggca tttgcattct
    22141 atgatcctct gggggccacc gggtaccggc aaaacaaccc tcgctgaagt gattgcccgc
    22201 tatgcgaacg ctgatgtgga acgtatttct gccgttactt ctggcgtgaa agagattcgc
    22261 gaggcgatcg agcgcgctcg gcaaaaccgc aatgctggtc gccgcactat tctttttgtt
    22321 gacgaagttc accgattcaa caaaagccag caggatgcat ttctgccaca tattgaagac
    22381 ggcaccatca cttttattgg cgcaaccact gaaaacccgt cgtttgaact taactcggca
    22441 ctgctttccc gtgcccgtgt ctatctgctg aaatccctga gtacagagga tattgagcag
    22501 gtgcttactc aggcgatgga agacaaaacc cgtggctatg gtggtcagga tatagttctg
    22561 ccagatgaaa cgcgacgcgc cattgctgaa ctggtgaatg gcgacgcgcg ccgggcgtta
    22621 aatacgctgg aaatgatggc ggatatggcc gaagtcgatg atagcggtaa gcgggtcctg
    22681 aagcctgaat tactgaccga aatcgccggt gaacgtagcg cccgctttga taacaaaggc
    22741 gatcgctttt acgatctcat ttccgcactg cataagtcgg tacgtggtag cgcacccgat
    22801 gcggcgctgt actggtatgc gcgaattatt actgccggtg gcgatccgtt atatgtcgcg
    22861 cgtcgctgtc tggcgatagc gtcggaagat gtgggtaatg ctgatccacg agcgatgcag
    22921 gtggcaattg cggcctggga ttgctttacc cgagttggcc cggcggaagg agaacgcgcc
    22981 attgctcagg cgattgttta cctggcctgc gcgccaaaaa gcaacgctgt ctacactgcg
    23041 tttaaagccg cgctggcgga tgctcgcgaa cgtccggatt atgacgtgcc ggttcatttg
    23101 cgtaatgcgc cgacgaaatt aatgaaggaa atgggctacg ggcaggaata tcgttacgct
    23161 catgatgaag caaacgctta tgctgccggt gaggtttact tcccgccgga aatagcacaa
    23221 acacgctatt atttcccgac aaacaggggc cttgaaggca agattggcga aaagctcgcc
    23281 tggctggctg aacaggatca aaatagcccc ataaaacgct accgttaatg ttatcgttgc
    23341 ggtaatgttg ttactgtatc cctgtggtcg caggctgtgg ccacatctcc catttaattc
    23401 gataagcaca ggataagcat gctcgatccc aatctgctgc gtaatgagcc agacgcagtc
    23461 gctgaaaaac tggcacgccg gggctttaag ctggatgtag ataagctggg cgctcttgaa
    23521 gagcgtcgta aagtattgca ggtcaaaacg gaaaacctgc aagcggagcg taactcccga
    23581 tcgaaatcca ttggccaggc gaaagcgcgc ggggaagata tcgagccttt acgtctggaa
    23641 gtgaacaaac tgggcgaaga gctggatgca gcaaaagccg agctggatgc tttacaggct
    23701 gaaattcgcg atatcgcgct aaccatccct aacctgcctg cagatgaagt gccggtaggt
    23761 aaagacgaaa atgacaacgt tgaagtcagc cgctggggca ccccgcgtga gtttgacttt
    23821 gaagttcgtg accatgtgac gctgggtgaa atgcactctg gcctcgactt tgcagccgca
    23881 gttaagttga ccggttcccg ctttgtggta atgaaagggc agattgctcg catgcaccgc
    23941 gcactatcgc agtttatgct ggatctgcat accgaacagc atggctacag tgagaactat
    24001 gttccgtacc tggttaacca ggacacgctg tacggtacgg gtcagctgcc gaaatttgct
    24061 ggcgatctgt tccatactcg tccgctggaa gaagaagcag acaccagtaa ctatgcgctg
    24121 atcccaacgg cagaagttcc gctgaccaac ctggtgcgcg gtgaaatcat cgatgaagat
    24181 gatctgccaa ttaagatgac cgcccacacc ccatgcttcc gttctgaagc cggttcatat
    24241 ggtcgtgaca cccgtggtct gatccgtatg caccagttcg acaaagttga aatggtgcag
    24301 atcgtgcgcc cggaagactc aatggcggcg ctggaagaga tgactggtca tgcagaaaaa
    24361 gtcctgcagt tgctgggcct gccgtaccgt aaaatcatcc tttgtaccgg cgacatgggc
    24421 tttggtgctt gcaaaactta cgacctggaa gtatggatcc cggcacagaa cacctaccgt
    24481 gagatctctt cctgctccaa cgtttgggat ttccaggcac gtcgtatgca ggcacgttgc
    24541 cgcagcaagt cggacaagaa aacccgtctg gttcataccc tgaacggttc tggtctggct
    24601 gttggtcgta cgctggttgc agtaatggaa aactatcagc aggctgatgg tcgtattgaa
    24661 gtaccagaag ttctgcgtcc gtatatgaac ggactggaat atattggcta atacccaatt
    24721 tttctgaatc taaaaagcgc ctgcgggcgc tttttttgtc tccctttgat accgaacaat
    24781 aattactcct cacttacacg taatactact ttcgagtgaa aatctaccta tctctttgat
    24841 tttcaaatta ttcgatgtat acaagcctat atagcgacct gctatagaaa taattacaca
    24901 atacggtttg ttactggaat caatcgtgag caagcttgag tgagccatta tgaaaacgaa
    24961 aatccctgat gcggtattgg ctgctgaggt gagtcgccgt ggtttggtaa aaacgacagc
    25021 gatcggcggc ctggcaatgg ccagcagcgc gttaacatta ccttttagtc ggattgcgca
    25081 cgctgtcgat agcgccattc caacaaaatc agacgaaaag gttatctgga gcgcctgtac
    25141 agttaactgt ggtagtcgct gcccgctacg tatgcacgtc gtggacggtg aaatcaaata
    25201 tgttgaaacg gataatactg gcgatgacaa ctatgacggc ctgcaccagg tgcgcgcctg
    25261 tctgcgtggt cgttccatgc gtcgccgtgt ctacaatccg gaccgcctga aatatccgat
    25321 gaaacgagtc ggagcgcgcg gtgaaggcaa attcgagcgc attagctggg aagaagccta
    25381 cgacatcatc gcgaccaata tgcagcgcct gatcaaagag tacggcaacg aatccatcta
    25441 tctgaactat ggcaccggta cgctgggtgg caccatgacc cgctcctggc cgccgggaaa
    25501 taccctggtc gctcgcctga tgaactgctg cggcggttat ctgaaccatt acggtgacta
    25561 ctcttccgcg caaattgcgg aaggtttgaa ctatacctac ggcggctggg cagatggtaa
    25621 cagcccgtcg gatatcgaaa acagtaaact ggtagtgctg tttggtaata accctggcga
    25681 aacgcgaatg agtggcggtg gggtgactta ctatcttgaa caggcgcgtc agaaatctaa
    25741 tgctcgcatg atcatcatcg atccgcgcta taccgacacc ggagccgggc gcgaagatga
    25801 gtggatccct attcgtccgg gaacagatgc cgcactggtt aacggtctgg cgtatgtcat
    25861 gatcaccgaa aacctggtgg atcaggcatt cctcgataaa tattgcgttg gctacgacga
    25921 gaaaacactg cctgccagtg cgccgaaaaa tggtcactat aaagcttata ttctgggtga
    25981 agggccagat ggcgtagcta aaacgccgga atgggcctcg caaatcaccg gtgttccggc
    26041 agacaaaatc atcaaattgg ctcgtgaaat cggtagtacc aaaccggcgt ttatcagcca
    26101 gggatggggc ccgcagcgtc acgctaacgg tgaaatcgca acccgtgcta tctcgatgct
    26161 ggcgattctg accggtaacg ttggtattaa tggagggaac agcggcgcgc gtgaaggttc
    26221 atacagctta ccgtttgtgc gtatgccgac cctcgaaaac ccgatccaga ccagcatttc
    26281 tatgtttatg tggaccgatg ccattgaacg tggcccggaa atgacagcgc tgcgtgatgg
    26341 tgtgcgcggg aaagataagc tggatgtgcc gatcaaaatg atctggaact atgccggtaa
    26401 ctgcctgatt aaccagcatt ctgaaatcaa ccgtacccat gaaatccttc aggatgataa
    26461 gaagtgcgag ctgattgtgg ttatcgactg ccacatgacc tcatcagcga aatatgctga
    26521 catcctgctg cctgactgca ccgcttccga acagatggac tttgcactgg atgcatcctg
    26581 cgggaatatg tcttacgtga ttttcaacga tcaggtgatt aaaccgcgct ttgaatgtaa
    26641 gaccatctat gaaatgacca gcgaactggc aaaacgtctt ggcgttgagc aacagtttac
    26701 tgaaggccgt acacaggaag agtggatgcg gcatctgtat gcccagtcgc gggaagcgat
    26761 tcctgaactg ccaacgtttg aagagttccg caagcagggg atctttaaaa agcgcgaccc
    26821 acaagggcac cacgttgctt ataaagcttt ccgtgaagat cctcaggcaa atccactgac
    26881 cacgccatcg ggtaaaattg agatttattc gcaggcgctg gctgacattg ccgcgacctg
    26941 ggaattgcca gaaggcgatg tgatcgatcc actgccgatc tatacgccgg gctttgaaag
    27001 ttatcaggat ccgctgaaca aacaatatcc gctgcagctt actggcttcc actacaaatc
    27061 tcgcgttcac tcaacttacg gcaacgttga tgtgctgaaa gcggcttgcc gtcaggaaat
    27121 gtggatcaac ccgcttgatg cccaaaaacg cggtatcaac aacggcgata aagtcaggat
    27181 ctttaacgat cgtggtgagg ttcatattga ggcgaaagtg acgccacgaa tgatgccggg
    27241 tgtggtcgca ctgggtgaag gtgcctggta tgacccggat gcaaaacgtg tcgataaggg
    27301 aggttgtatc aacgtactga ccactcaacg tccgtctcct ctcgctaagg ggaatccgtc
    27361 acatacaaac cttgttcagg ttgaaaaggt gtaaggagta accgatgaca acccagtatg
    27421 gattttttat tgattccagc cgttgcaccg gttgcaaaac ctgcgagctg gcctgtaaag
    27481 actataaaga tctgacgccg gaagtcagct tccgccgcat ttatgaatat gctggtggcg
    27541 actggcagga agataacggt gtctggcacc agaacgtgtt tgcctactat ctgtcgattt
    27601 cgtgcaacca ctgcgaagat ccggcttgta ctaaagtctg cccgagtggt gcgatgcata
    27661 aacgtgaaga tggttttgtt gtagtcgatg aagatgtgtg cattggctgc cgctactgcc
    27721 atatggcttg cccgtatggc gcaccgcaat ataacgaaac gaaaggccat atgaccaaat
    27781 gcgatggttg ttatgaccgt gttgcggagg ggaaaaagcc gatctgtgtt gaatcttgcc
    27841 cgctgcgggc gctggatttc ggccctatcg acgagctgcg taaaaaacat ggcgatctgg
    27901 cggccgttgc gccgttgccg cgagctcact ttaccaaacc gaatattgtg atcaaaccca
    27961 atgccaacag ccgcccgacc ggggatacca ctggctatct ggcaaacccg aaggaggtgt
    28021 gagatgggaa gtggatggca tgaatggccg ctgatgatct tcacggtctt cgggcaatgt
    28081 gtagcgggtg gttttatcgt tctggctttg gcgctgctca aaggcgacct gcgagcagaa
    28141 gcccagcagc gtgttattgc ctgcatgttt ggtttatggg tgctgatggg cattggcttt
    28201 atcgcctcta tgcttcatct tggttcaccg atgcgcgcct ttaactcgct caaccgggta
    28261 ggggcttcag cactcagtaa cgaaatcgcc agcggttcga tcttttttgc tgttggtggc
    28321 atcggctggt tgctggcaat gctgaaaaag ctgtcaccgg cattgcgtac gctctggctg
    28381 atagtgacga tggttcttgg cgtcatcttt gtctggatga tggtgcgtgt gtataacagc
    28441 attgataccg ttccgacctg gtacagcatc tggacgccga tgggcttctt cctgacgatg
    28501 tttatgggcg gcccgttact gggttacctg ttgctgagtc tggctggcgt cgatggctgg
    28561 gcgatgcgtc tgctgccagc catttctgta ctggccctgg tagtgagtgg cgtggtgtca
    28621 gtgatgcagg gcgcagagct ggcgactatt catagctctg tgcagcaggc cgcagcgctg
    28681 gtgccggact atggtgcgct gatgtcctgg cggatcgtgc ttttggctgt tgccctgtgc
    28741 ttgtggattg caccacagct aaaaggttat cagcctgcgg ttccgctact ttccgtatca
    28801 ttcattctgc ttctggcagg ggaattaatc ggtcgtggcg tattctatgg tttgcatatg
    28861 accgtgggga tggccgtcgc aagctaatca taacaaccgg ggtttcggcc ccggttctct
    28921 tttatttctg cttcgttaac gtgtcataac tggtcatcaa attacgataa tcaggaatat
    28981 ggttagagaa caacgtcgcc agtccttcaa tatcattacg ccagtcgcga tgcagctcgc
    29041 aagccacgcc gaaccatgtc atcaattgcg caccagcttg cgacatgcga tcccacgccg
    29101 aatgccgggt aatttcatta aaggtccctg acgcgtcagt cacgacaaag acgtcaaacc
    29161 cttcttcaat ggctgaaagc gccgggaatg ccacgcaaac ttcggttacc acaccggcaa
    29221 taattaactg ttttttacct gtcgctttga ctgcttttac aaaatcttcg ttatcccagg
    29281 cgttaatatt gcccgggcga gcaatgtaag gtgcatcggg gaattgtgct ttcagttctg
    29341 gaactaatgg gccgttagga ccggtttcaa aactggtggt aagaatagtt ggtaagttga
    29401 aatacttagc caggtcaccc agcgccagca cgttattttt aaacttatcg ggttcgatat
    29461 cccgtacaag ggaaagtaaa ccagcctggt gatcaacaag caaaacggca gcatcatttt
    29521 tatcaagacg aacatacggt ttggtcatcc tcttctcctt tcgagagtca gtggtacgtc
    29581 tgaaatcggt cagacgcgat gcattgctct gaaagcatag acgggaaata tgagtttgct
    29641 gtgaccatga aatttttcga ctgaacgaag cgtatgaaat ttgtgttaga tcaataaaaa
    29701 caatcagatg agattaatcg tctctccagc attagcgtta tgaatgagta atataaatcg
    29761 ataatacctc cgctgcgata tgcgcgtatt ttcacccatt gacaatgttt ttggcggtgg
    29821 catgatgcgc atgaaatttg aacttcctca cggttttaat tcatgtccac gtatacccgg
    29881 cctgtcatgc ttttgctgtc tggcctgctt ttgttgactc tggcgatcgc agtgttaaat
    29941 acactcgtgc cgctttggct cgcccaggaa cacatgtcca catggcaggt aggcgttgtc
    30001 agctcatcct attttaccgg caacctggtc ggtacattgc tgacagggta tgtcattaag
    30061 cgcattggct ttaaccgcag ctattatctg gcctccttca tttttgccgc tggctgtgcc
    30121 ggccttggcc tgatgattgg attctggagc tggttggctt ggcgttttgt cgccggcgtc
    30181 ggctgtgcca tgatttgggt ggttgttgag agcgcgctga tgtgcagtgg gacgtcacgt
    30241 aaccgtgggc gtttgcttgc tgcgtatatg atggtttatt acgtgggaac gtttttaggc
    30301 cagttactgg tcagcaaagt ttcaaccgag ctgatgtccg tattgccgtg ggttacaggt
    30361 ttgacgttgg cagggatctt accgctgttg tttacgcgtg tgctgaatca gcaggctgaa
    30421 aaccatgatt cgacgtcaat tacgtcaatg ctaaaactcc gtcaggcgcg gcttggcgtg
    30481 aatggctgca ttatctcagg aatcgttctg ggatctctat atggcctgat gccgctgtac
    30541 ctcaatcaca aaggggtgag caatgccagc atttgtttct ggatggcggt actggtcagt
    30601 gcgggtatcc ttggacaatg gccgattgga cgtctggcgg ataagtttgg tcgactgctg
    30661 gtgttgcgtg ttcaggtctt tgtcgtcatt ctcggcagta tcgcgatgct tagccaggcg
    30721 gcgatggccc cagcgttatt catcctcggt gccgctggct ttacgctata tccggtggcg
    30781 atggcctggg cttgcgagaa agttgaacat catcaactgg tggcgatgaa ccaggcctta
    30841 ctgttgagct ataccgtggg aagtctgctt ggcccgtcat ttaccgctat gctaatgcag
    30901 aatttctccg ataatttatt gtttatcatg atcgccagcg tatcgtttat ctatttgctg
    30961 atgctgcttc gcaacgccgg tcatacgccg aaacccgttg ctcacgtgta aatgaattca
    31021 agcagagtgt gaacttactg tttcacactc tgcttttttg tttcttctat ctgacttgct
    31081 ttattccaaa ttttattcgt ttaaaaataa aatgtgcagc aggttataat tttgcatttc
    31141 gctatttccg cacttcttat ttgccgcgca taatccctcg ttttacagat gcccctttaa
    31201 ttttggcgaa ggatttgtct atggctggga atgttcagga aaaacagctg cgatggtaca
    31261 acattgcgct gatgtctttt atcactgtct ggggttttgg caacgttgtt aataactacg
    31321 ccaaccaggg gctggtggtt gttttttcat gggtgtttat ctttgcgctc tatttcacac
    31381 cttatgcgct aattgttggt cagttaggct cgaccttcaa agatgggaag ggcggggtca
    31441 gtacctggat taaacacacg atgggacccg gactggctta cctcgccgcg tggacctact
    31501 gggtagtgca tattccctat ctggcacaaa aaccccaggc aatactgatt gcgctcggtt
    31561 gggcgatgaa aggcgacggt tcgttaatca aagaatattc agtcgtagcg ttacaggggt
    31621 taacgctggt gctgtttatc ttctttatgt gggttgcttc acgcggtatg aaatcgctga
    31681 aaatcgtcgg ttctgtggca gggattgcga tgtttgttat gtcgctcctg tatgtggcga
    31741 tggcggtaac cgcgcctgcg attactgaag tgcatattgc gaccacaaac attacctggg
    31801 aaacgttcat tcctcatatc gactttacct acattaccac tatttcaatg ctggttttcg
    31861 cggttggcgg agcagagaag atttctcctt acgttaatca aacgcgcaac ccaggaaaag
    31921 aatttccaaa agggatgtta tgccttgcgg tgatggttgc ggtttgtgcc attctgggct
    31981 cactggcgat ggggatgatg tttgactcgc gtaatatccc ggatgactta atgactaacg
    32041 gtcagtatta cgcctttcag aagctgggcg agtattacaa catgggtaat actttaatgg
    32101 tgatttatgc cattgcgaat accctgggac aagtagctgc gctggtattc tcgattgatg
    32161 cgccgcttaa agtgttatta ggcgatgctg acagcaaata tattccagcc agtttatgtc
    32221 gtaccaacgc ttctggtacg cccgttaatg gctattttct gaccctggtg ctggtggcga
    32281 tcctgataat gctgccgact ctcggcattg gtgatatgaa caatctctat aaatggctgt
    32341 tgaaccttaa ttcggtagtg atgccgctgc gttatttatg ggtatttgtt gcatttattg
    32401 cagttgttcg cctggcgcag aaatataagc cggaatatgt ctttattcgt aataagccgc
    32461 tggcaatgac cgtcggaatt tggtgttttg cctttaccgc ttttgcctgc ctgactggta
    32521 ttttcccgaa aatggaagcc ttcaccgcag agtggacatt ccagttggcg ctgaatgttg
    32581 caacgccgtt tgtgctggtg ggattagggc tgatattccc gctgctggcg cgtaaagcga
    32641 atagcaaata attattgtgg tcggtgacgc tgactctggc agatctgacc cgtttttaca
    32701 taatcgagaa atgcgcgcaa gccgcaggac atatactggc ggtttgggta atacatttgg
    32761 aagctgtgct gtaagggcgt tagaaataga gctctgaatt atttatgcag cgcggaataa
    32821 aaaagcggca actcaaaaaa gttgccgctt tacggggaaa ttagaacatt accttatgac
    32881 cgtactgctc aagaatgcct ttcacgcgtt ccatggtttc tttcttcggt ggtttaacac
    32941 catcaagttt gtattcttca cccatcgcca cccatttgtg tttgcccagt tcgtggtagg
    33001 ggagaagctc gattttctca acgttgccca tatcacgggt aaattcaccg aggcgatgcg
    33061 ctgaatcgtc atcgtcagac cagcctggca caacaacgta gcggatccac accttcacat
    33121 ttttgttcgc cagatatttg gcgaactcca gcgtgcggtg gtttgaaact ccaaccagat
    33181 tttggtggat ctcgtcgttc atctgtttga gatcgagcat taccaggtcg gttacttcca
    33241 gcagttcatc aatcaccgga tcgtaacgac gaacaaaacc gttggtgtcc agacaggtat
    33301 gaatgccttc ttttttgcag gcgcggaacc agtcacgaac aaactcagct tgcaggattg
    33361 cttcaccgcc ggatgcggta acgccgccgc cggaagcgtt cataaagtgg cgataggtca
    33421 ccacctcttt cattaaatct tcaacggtaa cttctttacc gccatgcgtg tcccaggtgt
    33481 cgcggttatg acaatacagg cagcgcatca ggcagccctg gaaaaaggtg ataaagcgaa
    33541 tacccgggcc gtctacggtt ccacaggatt caaaggagtg aatgcgacca ataactgaca
    33601 ttgcggtgtt tctccagatg tggcccatct gaggccgtgt tggtgcgcag ctcgaaggct
    33661 acgtcgagtc tgttttggca gtcaccttaa agtatagata gctgacaaaa aaggctctcg
    33721 tgctaaaaaa ggccccactt tcgtggagcc tttattgtac gctttttact gtacgatttc
    33781 agtcaaatct aattacatag attgagtgaa ggtacgagta ataacgtcct gctgctgttc
    33841 tttagtcagc gagttgaaac gtactgcgta gccagataca cggatggtca gctgcggata
    33901 tttttccggg ttttccatcg cgtcgagcag catttcacgg ttcatcacgt taacgttcag
    33961 gtgctgacca ccttcgatgg atgcttcgtg gtggaagtaa ccatccatca gaccagccag
    34021 gttggtctta cgaacttcgt cgtctttacc cagtgcgttc ggaacgatag agaaggtgta
    34081 ggagatacca tctttagcgt aagcaaacgg cagtttagca acggaagtca gagaggctac
    34141 tgcacctttc tggtcacgac cgtgcatcgg gttagcaccc ggtccgaacg gcgcgccagc
    34201 acgacgaccg tctggggtgt taccagtttt cttaccatac acaacgttag aagtgatggt
    34261 cagaacagac tgagtcggga tagcgtcacg gtaggtgtgc agtttctgaa ttttcttcat
    34321 gaaacgttct accaggtcaa cagccaggtc atctacacgc ggatcgttgt taccaaactg
    34381 cgggtattcg ccttcgattt cgaagtcgat agccagaccg tcttcgtcac gaatcggttt
    34441 aactttcgca tatttgattg cagacaggga gtcagcagca acggacagac cagcgatacc
    34501 acacgccatg gtgcggataa cgtcacggtc gtgcagcgcc atcagagagg cttcgtagct
    34561 gtacttgtcg tgcatgtagt ggatgatgtt cagtgcagtg atgtactgtt tagccagcca
    34621 gtccatgaag tgatccatgc gctccatcac ttcatcgtag ttcaggagat cgcctttgat
    34681 cggctcagac ttcggaccaa cctgcatttt cagtttttcg tcaacgccgc cgttgattgc
    34741 gtacagcatg gttttcgcca ggtttgcacg cgcaccgaag aactgcattt gtttaccaac
    34801 gatcatcggg cttacgcagc aagcgatagc gtagtcatcg ttgttgaagt ccggacgcat
    34861 caggtcatcg ttctcatact gcagagaaga ggtgtcgatg gacactttag cagcgaattt
    34921 cttgaagttc agcggcagtt tttcggacca cagaatggtc atgttcggtt ccggagacgg
    34981 acccatggtg tacagggtgt tcaggaaacg gaagctgttt ttggtaacca gggtacgacc
    35041 gtcgaggccc ataccaccga tagattcggt tgcccagatc gggtcgccag agaacagttc
    35101 atcgtattcc ggagtacgca ggaagcgaac catacgcagt ttcatgacca ggtggtcaac
    35161 catttcctgc gcttcttgtt cggtgatctt gccagctttc aggtcacgtt cgatgtacac
    35221 atccaggaag gtggaggtac gaccgaagga cattgcagca ccgttctgag acttaacagc
    35281 agccaggtag ccgaagtaag tccactggat agcttcctga gcgttggtag ccggaccaga
    35341 gatgtcgtag ccgtatttcg cagccatttc tttcatctga cccagagcgc ggtgctgttc
    35401 agcgatttct tcgcgcagac ggatagtctg ttccaggttt acgccgtttt ccagatcagc
    35461 ctgcagagag gtgaactgtg caagtttatc tttcatcagg tagtcgatac cgtacagcgc
    35521 aacgcgacgg tagtcaccga tgatacgacc acggccataa gcatctggca gaccggtcag
    35581 aacaccggat ttacggcaac gcaggatgtc cggagtgtaa acgtcgaaca cgccctggtt
    35641 gtgagtttta cggtattcag tgaagatttt tttgatcatc gggtccagtt cgcggttgta
    35701 cgctttgcag gaaccttcga tcattttgat accaccgaac gggataagag cacgtttcag
    35761 cggagcttca gtctgcagac caacaacttt ttccaacgct ttgttgatgt agccagcgtc
    35821 gtgagaggtg atggtggaag caacagcggt gtcaaagtca actggcgcgt gagtgcggtt
    35881 ttccagttta acgccttcca ttactttgtc ccacagggtg gtggtcgctt cagtagcgcc
    35941 agccaggaag gactcgtcac cctcgtacgg agtgtagttt ttctgaatga agtcacggac
    36001 gtttacttca ttctgccagt cacctttggt aaaaccttcc caggctgtgg ctaacttttc
    36061 attaagctcg gacatgtaac acctaccttc ttaagtggat tttttattta ctgcgtactt
    36121 cgacaaccat taatggtgat cgttttcacg caggtaaatg acccagtatg tcaacccaac
    36181 caacaaacca ccgccgataa tattaccgat cgtaaccgga atcaggttat cagtgatgaa
    36241 gttcatcacg gtcaggtgag aaaaattttc cggtgcagaa ccgacagcgg tccagaattc
    36301 cggagatgcg aagtcgcgga ttacaatacc catcgggatc ataaacatgt ttgcgatact
    36361 gtgctcaaaa ccgctggcaa caaacatcgc gaccggcagc accataatga acgctttatc
    36421 catcaggctg cggccagaat aactcatcca tactgccaga cataccatca ggtttgccag
    36481 gataccaagg cagacggcct caataaaagt atggtgcact ttgtggtcgg cggtttgtag
    36541 gacgtttagt ccccattgac cattcgcggt catatactcg ccggaaagcc acattaaaag
    36601 tacaaacagc agtgcgccga ccaggttgcc aaaatagaca tttagccagt ttttcgccaa
    36661 ctgaccccag gtgattcgcc cactcgcctt agcaacaaca atcaacactg tggaagtaaa
    36721 gagatcggct ccgcagacaa cacaaagaat cagccccaga gagaagcaaa tgccgccaac
    36781 cagttttgcc atgccgaagg gcattgttcc tgtgccagtg gttgctgtga tatagaagac
    36841 gaatgcgatt gagatgaaaa caccggcggt aatcgccaga tagaaagtct taagcggatg
    36901 tttcgttgct ttatagacac ctgcctcttc ggccactttg gccattgctg caggaagtaa
    36961 aagatcaaaa gggttgtcag ctttcacact aactctctct ttattaagtc ggcgacgaga
    37021 tactaacaaa gcattataga tgagaaattg atatagatca tatctcgcct ggcttatagg
    37081 cccgtaactc gcatggtttt tatgcaaata cggagtaaat
//
LOCUS       DACBVI010000030        35789 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.030, whole genome shotgun sequence.
ACCESSION   DACBVI010000030 DACBVI010000000
VERSION     DACBVI010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.030"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(201..602)
                     /gene="nikR"
                     /locus_tag="IU325_07420"
     CDS             complement(201..602)
                     /gene="nikR"
                     /locus_tag="IU325_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HAP5808862.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(607..1413)
                     /gene="nikE"
                     /locus_tag="IU325_07425"
     CDS             complement(607..1413)
                     /gene="nikE"
                     /locus_tag="IU325_07425"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HAP5808863.1"
                     /translation="MTLLNVCDLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKAEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAYPVRRRATEKV"
     gene            complement(1410..2174)
                     /gene="nikD"
                     /locus_tag="IU325_07430"
     CDS             complement(1410..2174)
                     /gene="nikD"
                     /locus_tag="IU325_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HAP5808864.1"
                     /translation="MPQQIELRDIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2174..3007)
                     /gene="nikC"
                     /locus_tag="IU325_07435"
     CDS             complement(2174..3007)
                     /gene="nikC"
                     /locus_tag="IU325_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HAP5808865.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(3004..3948)
                     /gene="nikB"
                     /locus_tag="IU325_07440"
     CDS             complement(3004..3948)
                     /gene="nikB"
                     /locus_tag="IU325_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HAP5808866.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(3948..5522)
                     /gene="nikA"
                     /locus_tag="IU325_07445"
     CDS             complement(3948..5522)
                     /gene="nikA"
                     /locus_tag="IU325_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HAP5808867.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(5633..6220)
                     /gene="acpT"
                     /locus_tag="IU325_07450"
     CDS             complement(5633..6220)
                     /gene="acpT"
                     /locus_tag="IU325_07450"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HAP5808868.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLDNLSLAICTPTPFTLSADSVQWIDSVN"
     gene            complement(6275..7324)
                     /locus_tag="IU325_07455"
     CDS             complement(6275..7324)
                     /locus_tag="IU325_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP5808869.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHISPERILQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            7456..8673
                     /locus_tag="IU325_07460"
     CDS             7456..8673
                     /locus_tag="IU325_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5808870.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(8677..9234)
                     /gene="dcrB"
                     /locus_tag="IU325_07465"
     CDS             complement(8677..9234)
                     /gene="dcrB"
                     /locus_tag="IU325_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HAP5808871.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(9307..9972)
                     /locus_tag="IU325_07470"
     CDS             complement(9307..9972)
                     /locus_tag="IU325_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HAP5808872.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10194..10439
                     /gene="tusA"
                     /locus_tag="IU325_07475"
     CDS             10194..10439
                     /gene="tusA"
                     /locus_tag="IU325_07475"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HAP5808873.1"
                     /translation="MTDLFSCPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(10541..12739)
                     /gene="zntA"
                     /locus_tag="IU325_07480"
     CDS             complement(10541..12739)
                     /gene="zntA"
                     /locus_tag="IU325_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HAP5808874.1"
                     /translation="MSTPDNHGKKAPHFAAFKPLTTAQNANNCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQTSRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAIALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPAVAFAGLINELESAGQTVVLVVRNNDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(12813..13439)
                     /locus_tag="IU325_07485"
     CDS             complement(12813..13439)
                     /locus_tag="IU325_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HAP5808875.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            13580..13939
                     /locus_tag="IU325_07490"
     CDS             13580..13939
                     /locus_tag="IU325_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 family protein"
                     /protein_id="HAP5808876.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(13942..14211)
                     /locus_tag="IU325_07495"
     CDS             complement(13942..14211)
                     /locus_tag="IU325_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HAP5808877.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14201..14797)
                     /gene="rsmD"
                     /locus_tag="IU325_07500"
     CDS             complement(14201..14797)
                     /gene="rsmD"
                     /locus_tag="IU325_07500"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HAP5808878.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            14947..16443
                     /gene="ftsY"
                     /locus_tag="IU325_07505"
     CDS             14947..16443
                     /gene="ftsY"
                     /locus_tag="IU325_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP5808879.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAAEVVEVTEQVAESEKAQPEAEVVAQPELVAEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWRAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            16446..17114
                     /gene="ftsE"
                     /locus_tag="IU325_07510"
     CDS             16446..17114
                     /gene="ftsE"
                     /locus_tag="IU325_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP5808880.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            17107..18165
                     /gene="ftsX"
                     /locus_tag="IU325_07515"
     CDS             17107..18165
                     /gene="ftsX"
                     /locus_tag="IU325_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HAP5808881.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            18410..19264
                     /gene="rpoH"
                     /locus_tag="IU325_07520"
     CDS             18410..19264
                     /gene="rpoH"
                     /locus_tag="IU325_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HAP5808882.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            19535..20638
                     /locus_tag="IU325_07525"
     CDS             19535..20638
                     /locus_tag="IU325_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein"
                     /protein_id="HAP5808883.1"
                     /translation="MNMKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(20838..21221)
                     /gene="panM"
                     /locus_tag="IU325_07530"
     CDS             complement(20838..21221)
                     /gene="panM"
                     /locus_tag="IU325_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HAP5808884.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            21645..22754
                     /gene="livk"
                     /locus_tag="IU325_07535"
     CDS             21645..22754
                     /gene="livk"
                     /locus_tag="IU325_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HAP5808885.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANKGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            22802..23728
                     /gene="livH"
                     /locus_tag="IU325_07540"
     CDS             22802..23728
                     /gene="livH"
                     /locus_tag="IU325_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HAP5808886.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            23725..25002
                     /gene="livM"
                     /locus_tag="IU325_07545"
     CDS             23725..25002
                     /gene="livM"
                     /locus_tag="IU325_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HAP5808887.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSISGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            24999..25766
                     /gene="livG"
                     /locus_tag="IU325_07550"
     CDS             24999..25766
                     /gene="livG"
                     /locus_tag="IU325_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HAP5808888.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            25768..26481
                     /gene="livF"
                     /locus_tag="IU325_07555"
     CDS             25768..26481
                     /gene="livF"
                     /locus_tag="IU325_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HAP5808889.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGNALLANEAVRSAYLGG"
     gene            26600..27346
                     /locus_tag="IU325_07560"
     CDS             26600..27346
                     /locus_tag="IU325_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP5808890.1"
                     /translation="MHYAVVVMLELLCCIHAYRTGQERYWIFIIFCFPVIGCVAYFVI
                     VMLPETGADRHGHTLLMRLQDKLNPERHLRKLTEELAIAETNQNHYALANELARLGRY
                     HEAVPHYQQALSGIFAHEAAMMLSLAQAQFAIQEFAACQQTLEDVMRYNPDFQSADGH
                     LLFARTLAAQEKYADAESEFEVLISYYPGPQARIYYAEMLAKMSRLREANEQYVAVVD
                     TAKRSRPHYRKHHREWIKTANERLKQSVVQ"
     gene            27706..29022
                     /gene="ugpB"
                     /locus_tag="IU325_07565"
     CDS             27706..29022
                     /gene="ugpB"
                     /locus_tag="IU325_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HAP5808891.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEQSTKS"
     gene            29198..30085
                     /gene="ugpA"
                     /locus_tag="IU325_07570"
     CDS             29198..30085
                     /gene="ugpA"
                     /locus_tag="IU325_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HAP5808892.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPHSFIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            30082..30927
                     /gene="ugpE"
                     /locus_tag="IU325_07575"
     CDS             30082..30927
                     /gene="ugpE"
                     /locus_tag="IU325_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HAP5808893.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            30929..31999
                     /gene="ugpC"
                     /locus_tag="IU325_07580"
     CDS             30929..31999
                     /gene="ugpC"
                     /locus_tag="IU325_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP5808894.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTTGDIWIDRKRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVREPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLAGRVNNEGTHFELDGGIALPLNGGYRQYAGRKM
                     TLGIRPEHITLRSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            31996..32739
                     /gene="ugpQ"
                     /locus_tag="IU325_07585"
     CDS             31996..32739
                     /gene="ugpQ"
                     /locus_tag="IU325_07585"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP5808895.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGDLNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARELWADMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(32726..33166)
                     /locus_tag="IU325_07590"
     CDS             complement(32726..33166)
                     /locus_tag="IU325_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HAP5808896.1"
                     /translation="MKRLLLLTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSPLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            33286..35028
                     /locus_tag="IU325_07595"
     CDS             33286..35028
                     /locus_tag="IU325_07595"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HAP5808897.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPPPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGAPLKK
                     GDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(35066..35350)
                     /locus_tag="IU325_07600"
     CDS             complement(35066..35350)
                     /locus_tag="IU325_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808898.1"
                     /translation="MITYHDAFAKANHYLDDADLPAVITLHGRFSQGWYFCFESQEFL
                     ESGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQVYEIKKATFGLP"
     gene            complement(35589..35705)
                     /locus_tag="IU325_07605"
     CDS             complement(35589..35705)
                     /locus_tag="IU325_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808899.1"
                     /translation="MEKLTTELHSLSEMDRRHVISILAEIANGYDDFSDMLI"
BASE COUNT         8334 a         9230 c         9963 g         8262 t
ORIGIN      
        1 taaaatcctt ttctattatt ttaatgacag tatcttcata cgaaaaagcg aagaaaaact
       61 tgtggtcaca atgaattgaa ggtttttagg aatatattta gaacgttaca aaagagaagg
      121 ttcggatagg gtatttagag aggaatcagg tgtgctagtg gccctttgtt gatcgttcac
      181 caaaggaccc aataataggc ttaatcttcc ttcggcaagc actgcaaatg cccgtgccgc
      241 acgccgcgct gggcgataac gtcatcggca aaatgctgca cgtcacccat gtcacctttc
      301 aacacggcga tttccagaca gtcgtcgtga ttgatgtgga catgtagcgt cgcgacggag
      361 agatcgtggt gatggtgctg ggtggagaca atacggctgg ctaagtcgcg tttttcgtgt
      421 tcatacacat acgacagcac cgcgaaacct tgtgtgccgt gctgctgagt ggcctcttgc
      481 gccagggcgc tacgcagaat atcgcggata gcttcggaac ggttgttata gccacgacgc
      541 tggctcaggc tgtccagcgt ctccagtaaa tcgtcatcaa gcgtgatggt aactcgttgc
      601 atagacttaa accttttctg tggcgcgacg gcgcacgggg tatgcgggta ataccgcgtt
      661 ttgtagcaca cgtccggcgt cagaggaaaa ggttaattta tctcccacca cctgggtttc
      721 gacgatttgc ccgttgtcca tcaccatcac tcgctggcaa aagcgttcca ccaggcgtaa
      781 gtcatgagtg atgaacaggc aggcagtgcc aaactgttgt tgtagctttt tcagcaggcg
      841 aatgacgccc gcctgtaaca cgagatcaag gttagaaacg gcttcatcca gaatcagcag
      901 tttcggttcg accgccaacg cgcgtgccag gcagacgcgc tggagctggc cgccgcttaa
      961 ctgcggtggg cgcttgtcga gcacgctgtc atcgagatcg accgccttca gcatttcgct
     1021 ggcgcgcgcc agttgttcgg cttttttcag cgatagcagg tggcgcatcg gttcacgcag
     1081 gatctcgcgc acggttttgc gcggattcac ggcactgatg gagtcctgaa acaccatctg
     1141 aatatcacgg cggaaggctt tacgttgggc gcgattaagt ttcgccagcg attcgccgcg
     1201 ccagctaata ttcccttgtg agggagactc taagcctacc agcagccgcg cgagggtgct
     1261 ttttccgcag ccgctgcgtc ccagcagggc gacggtttca ccgcttttca gggtcaggga
     1321 aacgttattc agtaccgcct gatgctgatg ttttccgcta aatccaccgt gcgcatagtg
     1381 atgtgaaagg tcgcagacgt taagtaaagt catgatgcca gctccatacc gtagagggcg
     1441 agatgagcgg aaaccaggct gcgcgtcacc gtatgtttgg gggcgttaaa cagcgtttct
     1501 acatcgccct gttcgacaat tttaccgtgt gacatcaccg ccacgtcatc cgccagacgc
     1561 gccacaacgc ccatatcatg ggtgaccagc agcattcccg gtgcttgttt ttgcataatg
     1621 ctttccagca gatcgaggat gcgcgcctgt gctaccacgt cgaggtcggt ggtcggttca
     1681 tcggcgatga taaacggcga ttcacacaac accgccatcg caatcatcat gcgctgcaac
     1741 atgccgccgc tcatctcgaa cgggtacagc ttcaacacgc gcgcggcgtt ttccaacccc
     1801 accgcttcta tggcagcggt aagcgtagcg tcatcggcgg gtttccccag cgccaggcag
     1861 gtttcacgcg cgtgggtgtg catggtgtgt agcggattaa aggcgctgcg cgggttctgc
     1921 atgatggtgg caattttgat gccgcgcagg gcacagggag aaaccggttt gccatcggct
     1981 aaaatttccc ccgccgtctg gcgaacgcct gcgggcagaa tgcccagcgt cgcggcgcag
     2041 gtcagtgatt tcccgctgcc gctaccgccg actaacgcca gcacacgccc gcgtttaagg
     2101 gttaacgata cgccgtgcac cagcggctgc gcggcctgta gcgcgatatc gcgcagttca
     2161 atctgttgcg gcatcagtgt gcgtgctccg tcaccagatg aggatccaga tgatcgcgca
     2221 gtgcgtcacc caccaggtta aaggccatca cgctgataaa cagcgccagc cccggccaga
     2281 acatttgcag cggctgggtc cagatatact gacgcgcgtc gttaatcatc acgccccatt
     2341 cggcggtcgg cgcggtcacg ccgaggccga ggaaggacat ccccgcgacg tggagcatca
     2401 tatgaccaat atccagcgtc gccagcacca gcagcgaggg gatcaccgcg cctgccagat
     2461 gatcgacaaa cacccgcaca tggcccgcgc cggaaagccg tgacgccagc acaaactcgc
     2521 gctggcgtag cgaaatcacc aggctgcgca ccatgcgcgc ataccacgcc cagtgcgaca
     2581 gggcgatggc gataatcacg ttggtcaacc cggtgccgag tacgccgacc ataaagaacg
     2641 acagaatcga cgtcgggaag gtcataaaca tatccgcgac gcgcatggtg gcctgatcaa
     2701 cgcgcccgcc aatcagcccg gcgctgccgc caataactag ccctaatgtc agcaccagca
     2761 gcaggcaggc cattaccgag ccgagcgaca cgcgggtcgc cgccatcagc cgcgagaaaa
     2821 tatcgcgccc taaatgatcg gtacccagcc agtgctgcgc atccggtgaa agcaggcgcg
     2881 acggcagatc aatcgcctgg gggtcatacg gcagccacca ctggctggtg agcgcaatca
     2941 gcgccagcag ggcgataatg atcagcgcca ggcgtaccga ccagcgggaa gagaggaaaa
     3001 agttcacgcg tgggctcctt catgacgacg aatgcgcgga tccagcgcgg cgttgagcaa
     3061 atcaacaatc aaattacaga ccacaaaaac caccaccatc atcagcgtaa agcactggat
     3121 caccggatag tcacggttaa aaatcgccga caccgcatag cgcccgacgc ctggccaggc
     3181 aaagatgttt tcgataatca tcgtcccgcc aatcagttcg ccaatgtgca tccccacggc
     3241 ggtgatcatc ggcagcgagg cattgcgcag aatatggcgg cgttcggtct gtttgtcgtt
     3301 caggccgcgt agccgcgccc aggtgacgtg acgctgaccg gcgacgtcca gcatactggc
     3361 gcgcagtagc cgtgcattaa tcgccagcga cataaaggca atggaaaccg caggcaaaat
     3421 gatgtgctgc cagccgccgt agcccatcgc gggtagccat tgcagatata ccgaaaacgc
     3481 catcaccagc aaaaacgcca gccagaagtt gggcatcgac acgccgagaa acgcgatgaa
     3541 acgtacggcg aaatccggca gacgatcgcg atggcgcgcc gcccagatac cgagcggcac
     3601 ggaagtgagc agaattaata ccagcgcggc acctgcaagt tccagcgtgg cgggcaggaa
     3661 gttcagcata tcgtccagca ccgggcgttg gctggcgaat gagataccaa agtcaagatg
     3721 cagcgccttc cacaaccagg tgccgtactg gacatacagc ggctgatcca gccccagcat
     3781 ggtgcgtgta gaggccagca tctccggcgt cggcggcagg ttagacagac gcagataatc
     3841 gagcgccggg tcgccggtgc cgaggcgcag catcagaaaa atgatcaccg aggcggcgag
     3901 caccatcggg atcagcagca gaaagcggcg taatacgtaa cgcaacatta aggtttcacc
     3961 ggtttaatct gttcgaacgg aatttcggtg gcgatcggcg cgtaggggat gttacccagc
     4021 tccggttttg ataccaccat cattgagatg taactgatag gcagataaac cgcctcgtca
     4081 tgcagacggg tcagaatgtc gcgatacagc gcctgacgtt gcgtttcgtc atgggttgcc
     4141 agcacttcgc cgatctcttt atcaatcagc ggcttgtcgg ctaatccttg ctgtgcctgg
     4201 aaatcagcgt gcgacggtac gcgcattgaa ctcaggaagg cgtgtggatc gtatggcgcg
     4261 ccccaggtgc gatggaaaat catgccaaaa cgaccgtcgc gctgacgagc atagatactg
     4321 ctctcttctt cgccaatcag tgagacatct gcgccaatct ggcgcatatc agcctgaatg
     4381 atttccgcca tcgatttgct taacgcatcg gtgccgatga acgacagttc aatgcgcagc
     4441 ggctgaccat ttttctcgcg gatgtctttg cccgcaggca acgtccaacc ggctttttcc
     4501 agcaacgctt tcgctttctg cggatcgtac tggcgcggtt tcaggccgag gttggcatag
     4561 ggcacagaag gagcaaacag ggtgtcggcg acctgctggg tgccatacaa cgcgttatca
     4621 atcagcgatt ttttgtttac cgcgtaatta agagcttcac gtactgccag ctcgttggtg
     4681 ggggctttgg cggtattgag cgccagcatc acggtttcga tcggctgtga cagttgggtg
     4741 tggtaagccg ggttctggct aaagcgggcg aaggtatcga gcggtaataa cccttcgttt
     4801 ccgtacagca ggtcaatatc gccagtttca aacgccaccg cgcgggtagt cgggtccggg
     4861 atcacgttaa aagtgatctt cttaatcgcc ggcttttcgc cccagtagtt ttcgttacgg
     4921 acgaagacat cgtactgatt cagtttcgat tcctgcaaaa cccacggccc ggtgccaatc
     4981 ggcgctttaa ttccgttcat ggtttcatgg tttttaaact gcgagggggc gataaaacgg
     5041 aaagggcgtg gcagggccag ttcttgcagg aaaggatagt aggcgctttt cagggtgatt
     5101 tgcagctctg ttttactgag tgctttgaca tcaacaatct ggtttgccag ctccagccag
     5161 gcgtgacgtt gacggttatc gagcactgcg cggaagtttt ctgccgccgc ctctgcatca
     5221 aacggttcgc cgttggagaa tttcacgtca tcacgcaggg tgaaggtcca ggttttacca
     5281 tcttcagaat gagtccagct ttttgccagc cacgggatca ccgaaccgtc tgcctgatat
     5341 ttcaccaatg gttcataaac catgctctgg gcgaacatct ggttaggcgt gtaaaggtgc
     5401 gggtttagtg gcccgacatt caccggccag gcggtggtga tttcatctgg tgcagcggca
     5461 tggacgataa aagacgcaca agccagcagc gcaaatagag tgcggcggag tgtggagagc
     5521 atggtaaccc caatggatta aaatagatgg cggaaataag tatgacgatt ttaagtattc
     5581 gtcatactga taacctgttc tggatcaaaa gatggacagt cgggtgggcg gatcagttaa
     5641 ctgaatcgat ccattgcaca ctgtcggcgg agagcgtaaa gggggtaggg gtgcagatcg
     5701 ccaggcttaa attatcaagc tggcaatggc tgaccgacag tgatgagtga taggtgctgt
     5761 ctacgctgac gatttgccag gcgctgccgc cacgttgttt aacgatggct tctttgcgcg
     5821 tccagatgcg ccagaacatt tccagttgct gatcgggatg cacggcgtcc atctcagcgt
     5881 gttccccgag gctgaacacg gcgttcgcca gccagcgcca gttggcgcgc gggcgaatta
     5941 cttcgatatc gcagccgact tcgccttcat cgctcaacag cagggcgata tcgtcaccgc
     6001 tatggcttaa gttgaaccat agcggcgttt ccggcgcgaa tgcaggtttg ccttgttcgc
     6061 catagatgat ctccggtagc ggggaaagcg tgtgcgaaag caatgcacgc cccgccagcc
     6121 agcgttcacg tcgtggacct tgcggtgctt gctcgcgtaa acccggtggc agaggagctg
     6181 cgcttaaggt cgaaactttc cccagaacta tccgatacat atcagggcca acgtttaatg
     6241 gaaaatgaaa gtgcgtatcg taacacttgt cgcctcatcc cggtaaccga cttttcggtc
     6301 tgcctggtcc cagtaaaatc gccagtttgc taccgccttt ggtggtttcc atccagattt
     6361 tacacacgct ggttaatggc accgaaagta gcatccctac cgggccgagc agccatcccc
     6421 aaattaacaa tgaaagaaat accaccatgg tggacatccc caggcgatgg cccatcatcc
     6481 gtggctctaa aatattgccg atgaccatat ggacaactaa aaacaatgcg ccgaccagaa
     6541 tacattcgta aataccatta aacagcagca cctgaatcat tggcggtacg gcggaaatta
     6601 ccgcgccgat attgggcacg tagttgagca aaaacgccag tactgcccac atcagcgcaa
     6661 actgtacgcc cattagcgcc agccccagcc agacgatgac acctgtccat aaactgagta
     6721 acgtctttaa cgccagataa tgcgaaacgc ctttaagtgc gcggtgtaat cccgcgatgt
     6781 gaatctgtgg gttatttagc gcaaaacgca ttttgtaagg gacgtggcgc acttcaaaca
     6841 gcatgaaaac tacggtcatc accagcaaaa gcacgctcgc cattgccccg gaaagcccgg
     6901 tcattagcgc cgtggtgaag gtaaccactt tttccgagtc catccgctgc agtattcgct
     6961 ccggcgatat atgcaaatta agaaaaggca acatctcctg caatttaaaa agtttgcgcg
     7021 tcagctcctt attaaacttc ggcagcatag atataaattc gttaaacgat gccgccagta
     7081 cgccgaccag cgcggttagt gcgatcagca tcaccaccac cacaatcgta atggcaacgg
     7141 ggcgttgtac cccccgacga ataaaccagg tgacgagcgg gttgagaacg atggcaaaaa
     7201 acagcgccag taatagctgc acaatgatat ctgccgctgc gtgaatgccc gcgaggatca
     7261 ctaccagcga ggccagcttg agcagaatgt gcatgcccgt tttatcgggt tgaggggttt
     7321 ccattggggc ttccttgtga ctttttgtat taagtgtagc gggagtcacg ccagcaatat
     7381 tctgattctt actgctgatt ttcacgtcag cacgtggtaa aaatgaaaca ctgttgtaaa
     7441 aatgtggtga tcctcatgcc cgaacccgta gccgaacccg cgctaaacgg attgcgcctg
     7501 aatttgcgca ttgtctccat tgtcatgttt aacttcgcca gctacctcac catcgggttg
     7561 ccgctcgctg tattaccggg ctatgtccat gatgtgatgg gctttagcgc tttctgggca
     7621 ggattggtta tcagcctgca atatttcgcc accttgctga gccgccctca tgccggacgt
     7681 tatgccgatt tgctgggacc caaaaagatt gtcgtcttcg gtttatgcgg ctgctttttg
     7741 agcggtctgg gatatctgac ggcaggatta accgccagtc tgcccgtcat cagcctgtta
     7801 ttactttgcc tggggcgcgt gatccttggg attgggcaaa gttttgccgg aacgggatcg
     7861 accctatggg gcgttggcgt ggttggttcg ctgcatatcg ggcgggtgat ttcgtggaac
     7921 ggcattgtca cttatggggc gatggcgatg ggtgcgccgt taggtgtcgt gttttatcac
     7981 tggggcggct tgcaggcgtt agcgttaatc attatgggcg tggcgctggt ggccattttg
     8041 ttggcgatcc cgcgtccgac ggtaaaagcc agtaaaggca aaccgctgcc gtttcgcgcg
     8101 gtgcttgggc gcgtctggct gtacggcatg gcgctggcgt tggcttccgc cggatttggc
     8161 gtcatcgcca cctttatcac gctgttttat gacgctaaag gttgggacgg tgcggctttc
     8221 gcgctgacgc tgtttagctg tgcgtttgtc ggtacgcgtt tgttattccc taacggcatt
     8281 aaccgtatcg gcggcttaaa cgtagcgatg atttgcttta gcgttgagat aatcggcctg
     8341 ctactggttg gcgtggcgac tatgccgtgg atggcgaaaa tcggcgtctt actcgcgggg
     8401 gcagggtttt cgctggtgtt cccggcattg ggcgtagtgg cggtaaaagc ggttccgcag
     8461 caaaatcagg gggcggcgct ggcaacttac accgtattta tggatttatc gcttggcgtg
     8521 accggaccac tggctgggct ggtgatgagt tgggcgggcg tcccggtgat ttatctggcg
     8581 gcggcgggac tggtcgcaat cgcgttatta ctgacgtggc gattaaaaaa acggcctccg
     8641 gaacacgtcc ctgaggccgc ctcatcatct taaaacttac tgaataacca gcgtattaat
     8701 gatgttttct gcggtggtct gcgctttttg ctgatcgtca gctggcagag taatttgcat
     8761 ggtcagcagt tgattaccca cgttacccag aataacggaa gagtacgccg tctggccttt
     8821 cgcggagata atgctgtcta actgctgcat tttgtgacct ttcagctcaa tggctttatt
     8881 ggttaccact tgcagctgcg gatcgcggct acgttgctga tcttccagac gcttcgccag
     8941 caccgccaga tcttctttcg gatcatcgcc catgatgacg atgactgctt tctgcccggt
     9001 ggcgtcggac cagacatgca tgttattggc ctgcgttccc agcttaccgc tctggtcggt
     9061 catatccgct ggcagcgaga aacttaactt gccatcaagc aggttgacgg gattcccggt
     9121 agcgttactt tccgcgaccg aaccctgcgc cgtagcgtta gtgtctttat catcacaggc
     9181 cgcaagcccc ataaccagca ggccaattcc gacatattta accagattgc gcattgactt
     9241 cttcctttcg ataaacggcc ataacggctc attcatccat cttatcacaa ctctgataac
     9301 gaacctttaa ctcgcctgca aagcgttgat ttcggattta tctgccagtc ttttcaacag
     9361 catattgagt aatacgccat acattggcag gaagaaaacg atactgatta acactttgaa
     9421 acagtaatcg accagtgcga tttccatcca gtgttcagcc ataaaggcat ccgggctacg
     9481 ccagaaggca atgaagaaga aggccagcgt gtcgctgacg ttaccgaaca gtgtggacgc
     9541 tgtcggtgcc agccaccagc ggcgactctg acgcaggcgg ttaaaaacgt gcacgtcgag
     9601 gatctgtccc agcgcgtagg ccatgaaact ggcggtggcg atacgggcga caaacaggtt
     9661 gaagtgggcg agcgcgccga atccttgcca tgaccccata taaaacagcg atgagatgac
     9721 gtaggagatc agcaacgccg ggatcatcac cgcaaagatg atacgacggg ccagcggtgc
     9781 gccaaaaata cgcacagtca ggtcggtagc aaggaaaata aacggaaagc taaatgcgcc
     9841 ccaggtggta tggaaaccaa aaatggagac gggaagctga accagatagt tactggaggt
     9901 gatcaccagc agatgaaata acgataacca gaacaacgcc ttatagcgtt gagtttgcga
     9961 gaaaacgttc atattgtacc tttttgatta accattgggg tgagggaacc caatacgtac
    10021 gacacgctat tttaccttga ttaaataacg ttcttaccct tttttcgagc cgccgcatga
    10081 tactgcttta cgttgacaat gcaatggtta attttcacgc aatcgttaac ctggtttgct
    10141 tacggactca cagggcgtaa actagcgccg tttttttatg tgatgagaag aaaatgaccg
    10201 atctcttttc ctgccctgac cacacactcg acgcacttgg cctgcgctgc ccggaaccag
    10261 tcatgatggt gcgcaaaacc gtgcgcaata tgcagccagg tgaaacgttg ctgattattg
    10321 ctgacgatcc ggcgacgacc cgcgatattc ctgggttttg tacctttatg gaacacgaac
    10381 tggttgcgaa agagacggat ggactgcctt atcgttattt gattcgcaaa ggcggttgat
    10441 ggggaaagat tggcttcgat gccgcctttt cccctcaccc taaccctctc cccagaggag
    10501 cgaggggacc gatcgcgctc aatgttgcga tcggtttgcc ttatctcctg cgcaacaatc
    10561 ttaacgcatt cgctgtcacc agcaccgtcg cacccgtatc tgccagcacc gccagccaca
    10621 ggccggtcat ccctaacagt gtggtgacga ggaagatccc tttcagcccc agcgcaatag
    10681 tgatgttctg gcggatattg gcgtgagtgg cgcgtgccag ttcaatcatt tgcaccaggc
    10741 cgcgcaggtg gttatgggtt aatgctgcat cggcggtttc cagcgccacg tctgtgccac
    10801 tgcccattgc aatcccgatg gcggcagctt tcatcgctgg cgcgtcgtta ataccatcac
    10861 cgaccatcgc cagcggcgca tgttgattca gctcggtcac cgctttgact ttatcttccg
    10921 gcaacaggcc cgctttaaat tccagcccca gttccccggc aattgccgcc gctgcgcgtg
    10981 gattatcgcc ggtgaggatc acccctttga cgcccagcgc gttcagttca ctgatggcag
    11041 ttgcagcatc ggcgcgcaga gtatcctgca atgcaatgat acccagcacg tcattgttac
    11101 gtactaccag cactaccgtt tgcccggcgc tttccagttc gttaattaaa ccagcaaatg
    11161 caacagccgg atgttttccg gcagcgcata tcaacacgcg ctcaccgtta acctgcgctt
    11221 caatgccaga cccaaccagc gcccgctgtg attcggcggc gggaatggcg agttcagcaa
    11281 cctgtgcttc gcgtacgatg gcttgcgcca gtggatgcgt cgcgccttgc tcgaccgccg
    11341 ctgccagtgt cagcagttca gattcactaa tacccgttgc cggatgaatc gcggtaacgc
    11401 gcggtttacc gacggtcagc gtaccggttt tatcaaacgc cacctgggta acacgaccta
    11461 gttgttccag cgccgcaccg cctttaatca acgccccacg acgcgctgct gctgccagcc
    11521 cggaggttat cgccgcaggc gttgagataa ctaacgcaca cgggcaacca atcagcagca
    11581 gcgtcagccc tttataaatc cactcttgcc agctggcggc aaacagcagc ggcggcacca
    11641 gcgttaccag cagggcgatg gccataatcg ctggcgtgta aatacggctg aaacggtcga
    11701 taaacctctc aatgggggcg cgacgctctt cggcttcttc gatcagtttc agaatccggt
    11761 caatggcgct ggcacccggt tctgacagca cttccagcgt taccagacgg tctacgctgg
    11821 tggctcctgc aggaacttta tcgcccgtcg cgcgctccac cggaatggat tctccggtca
    11881 gggcgctttc atcaaaactg gcaaacggtg agagcagttt accgtcggca ggcaaacgcc
    11941 cgcccgccgc aacttcaatc acatcgccag ggcgcaggct gttaatcgcc acctcttccc
    12001 gctcaccgtt acgcaggcgc gtggcggttt ctggtttcag cgccatcaac gcgctaacgc
    12061 cctgacgcgc gcggctggcg gcccagcctt ccagtcgttc accaatcaaa aacagcagca
    12121 acaccatcgc agcttcagcc gttgcgccaa taaacagcgc accaatagca gctacgctca
    12181 ttaacgtttc aatggcgaag taactgccgg atttgatcag ccgtaatgcc tgacgagcaa
    12241 tcgggtacag cccaaccagc gtggtcgcga taaacgccag ttgcccgaac ggatgattaa
    12301 actgctccag accccagctg attgccatca tcacgattag cgtaatcagc ggcaaattct
    12361 ctttcaggcg tgatgtttgc ggttcgtcgg cggcctgttc atcgcgcagg gaatagcccg
    12421 ctttttgcac cgcagattca acctgggcac ggatgtcatt gtcggcatcg accaccagtt
    12481 tttcggtggc gaacaacacc tgcacctgat tcacgcctgc aagctggcgc acggcatttt
    12541 ctaccttgcg cgcacaggcg gcgcagtcca tgccgctgac tttccagcta tagcgggtgc
    12601 cggagacgtt ttcagagagc gttggcgtgc tggaacatgc gccgtcgcag caacagttgt
    12661 tggcgttctg tgccgtggtt agcggtttga acgcagcaaa gtgaggggct ttcttgccgt
    12721 gattgtcagg agtcgacatg gcatcctccg gttaagtttt ttctcattaa ccgaaggata
    12781 cactctggag tcgactccag agtcaagttt tatcagagat acagcgagcg gacgatcagg
    12841 aagtgaccgg caaagtagca ggccgcagca atcgcgttat ccgcacggaa gcgtcggcga
    12901 tagtggctcc ccagccagac aaagttactg ataaatagca acgaagcgcc aacaaacgca
    12961 gagagcgccg gagcggtcgg acggaagaac cacagttcac ctgccagcca caccatcacc
    13021 agcgtcatgc cgataaacgt gcagataggc caacggtact cttccagccg cgtccagata
    13081 atcgccagta acagcgcccc cagaaccagc aacaccagtg gcagaggcca gaagaaagag
    13141 agcgtcatct gactggcgaa atagatggtg tacagcaggt gcgagaggaa aaacgcgccg
    13201 atggcgtaca tcagacgttg acgtggcaac agggttagcg catcgcccag cagtgaggcg
    13261 cacagccccg ccagcaccag atagctaatg gcgtcgaaca tcggcgcttg ccaggccagt
    13321 aacagcagga gaagaagggt taacggttta aacacccagc gttgccaggt tggcccacga
    13381 tacgatgcat ccacagatag ccatgcggaa agacagacag cgataaacga ccaaagcatc
    13441 ttagttcctt gaattcgtta ttttttcgta agcggaatgc ctacggtctt gcttccagtt
    13501 tagtggcgag ggtggacgga tgacaacacc ataactggca gggtatgctt aattccgcat
    13561 tttccgatga gggataaaga tgagcaaacc acctcttttc tttattgtta tcattggctt
    13621 aatcatcgtc gccgcatcgt ttcgttttat gcaacaacga cgggaaaaag ctgataatga
    13681 tatggcaccg ctccagcaaa agttggtggt ggtgagcaac aagcgggaaa aaccgattaa
    13741 cgatcgccgt tcgcgccagc aggaagtgac tccggcaggc accagtatgc gctatgaggc
    13801 aagcttcaaa ccgcaaagcg gaggaatgga gcagacgttt cgcctcgacg cccagcagta
    13861 ccacgccctg acagtgggcg ataaaggtac gctgagctat aaaggaacgc gctttgtcag
    13921 ctttgttggc gaacaataag gttatttttt aactttaaat ttcttctgcc agaccagcag
    13981 ttcaaacacg ccgaaaagga aaatccgtac cttttcggcg gtggtcatct gcgggccatc
    14041 tttcggcaag gtcgatttca atagcgccag ctgcatacca tgcatcagca cggtaaaaat
    14101 cagcgccacg ttaacaaaaa tattcagcgg gcgtgggaag ggatgcacca ggttgaggat
    14161 taaaaatccc caaacgcaga gcattaacaa acgaccaata ttaatcagca tcactttctc
    14221 cttgtgcttc gcgttgatac agccgataag ccacctgacc cgccactttt tcccgatgta
    14281 atgaccagtt tgccggaaca gtgggcagac cgttttcgac ttcgctttcg acataaatca
    14341 gggcttcgtc agccagccag ccgttatctt ccagtaaatt tatcgtctct tctaacaagc
    14401 cacggcggaa cggcggatcg acaaacacga tattatgcgg tgtgcctttt tgcgccagga
    14461 atgacatcgc gttgctgttc accacgcgtg cattgcctgc ttttagtgtc gccagattct
    14521 taattaactg ctgagaaacc gcgcgatcca tctcaatcaa cgtggccccc gcagcgtagc
    14581 gcgataacgc ttccagcccc agcgcgccgc tcccggcgaa gcaatccaga cattgggcgt
    14641 caacaatgac cggagccagc cagttaaaca acgtttcgcg tacgcggtcg gtggtggggc
    14701 gcagacctgg gctatcagga accgggagtt tacggcctcg ccactgcccg ccaataatgc
    14761 ggatttggcc gctgccggaa tgattcggtt ttttcatgat aaattgctca atccgccaga
    14821 taacacatac ttgcaggcgg tgatgtgaat taagttaagt gatagactat ttcatcattt
    14881 ttttagctgc tatgtacata gcgttaacgc tgtgccatga agcaacagcg aggagtgtag
    14941 tcgcaaatgg cgaaagaaaa aaaacgtggc tttttttcct ggctgggctt tggtcaaaaa
    15001 gagcagaccc cggaaaaaga gacagaagtt cagaatgaac aaccggttgt agaagaaatc
    15061 gttcaggcgc aagagcctgc gaaggcttcg gaacaagccg ttgaagagca gccgcaggcg
    15121 catactgagg ccgaagcgga aacttttgct gccgaagttg tggaagtcac tgaacaggtt
    15181 gctgaaagtg aaaaagcgca gcctgaagcg gaagtcgttg cacagccgga actggtcgca
    15241 gaagaaacgc cggagccagt ggctatcgaa cgtgaagagc tgccgttgcc ggaagacgtc
    15301 aacgccgaag aggtttcgcc agaagagtgg cgggctgaag cggaaaccgt agagattgtc
    15361 gaagcggcgg aagaagaagc ggctaaagaa gaaatcaccg acgaagagct ggaagcacag
    15421 gcgctggctg ccgaagcggc agaagaggca gtgatggtgg ttcctccggc agaagaagag
    15481 cagccggtag aagaaatcgc tcaggagcag gaaaaaccga ccaaagaagg tttcttcgcg
    15541 cgcctgaaac gcagcctgtt aaaaactaaa gaaaatctcg gttccggatt tatcagcctg
    15601 ttccgcggta aaaaaatcga cgatgatctg tttgaagagc tggaagaaca gctgttgatc
    15661 gccgatgtgg gcgtggaaac cacacgtaaa ataatcacca atctgacgga aggcgcatcc
    15721 cgcaagcagc ttcgtgatgc cgaggcgctc tatggcctgc tgaaagaaga gatgggcgag
    15781 attctggcga aagtcgatga gccgctgaat gttgaaggta aaacgccgtt cgtgatcctg
    15841 atggtaggcg tcaacggtgt gggtaaaacc acgacgattg gtaagctggc gcgtcagttt
    15901 gagcagcagg gtaaatcggt gatgctggcg gcgggcgata ctttccgtgc ggcagcggtt
    15961 gaacagcttc aggtctgggg tcagcgcaac aatattccgg tgattgccca gcataccggt
    16021 gcggattccg cctctgttat cttcgacgcc attcaggccg ctaaagcgcg taatatcgac
    16081 gtcctgattg ccgataccgc cggacgcctg cagaacaaat cgcacctgat ggaagagttg
    16141 aagaaaatcg tccgcgtgat gaagaaactc gacgttgaag cgccgcatga agttatgctg
    16201 actatcgatg ccagcaccgg gcagaacgcg gtaagccagg ccaaactgtt ccatgaagcc
    16261 gttggcttaa ccggcatcac gctaacgaaa ctggacggca cggcgaaagg cggggtaatt
    16321 ttctcggtgg ctgaccagtt tggtattcct atccgctaca ttggtgtcgg cgaacgtatt
    16381 gaggatttgc gtccgtttaa ggcggacgac tttatagagg cactttttgc ccgagaggat
    16441 taacaatgat tcgctttgaa catgtcagca aggcttatct cggtgggaga caggcgctgc
    16501 agggcgttac gttccatatg cagccgggtg agatggcgtt tctgaccggt cattccggcg
    16561 cagggaaaag taccctcctg aagctgatct gtgggattga gcggcccagc gccgggaaaa
    16621 tctggtttag cggccatgac atcacgcgtc tgaaaaaccg tgaagttccg tttctgcgcc
    16681 gccagattgg catgattttc caggatcacc atctactgat ggaccgtact gtctacgata
    16741 acgtggcgat cccgctgatt atcgctggtg ccagcggtga cgatattcgt cgccgggtgt
    16801 cggcggcact ggataaagtc gggctactgg acaaagcgaa gaacttccct attcagcttt
    16861 cgggcggtga acaacagcgt gttggcattg cccgcgcggt ggtgaataag cccgcggtac
    16921 tgctggcgga cgaaccgact ggtaacctgg acgacgcgtt gtcggaaggc attttacgtc
    16981 tgtttgaaga gtttaaccgc gttggggtaa ccgtattgat ggcaacgcac gacatcaacc
    17041 tgatttcgcg gcgttcctat cgcatgctca ccctgagcga tggtcacttg catggaggcg
    17101 tgggccatga ataagcgcga tgcaatcaat catattcggc agtttggcgg gcgtctggat
    17161 cgcttccgta aatcggtcgg cggctcaggc gacggcggtc gtaacgcgcc aaaacgcgcg
    17221 aaatcctcgc caaaaccggt aaatcgcaaa accaacgttt tcaacgaaca ggtgcgctat
    17281 gccttccacg gcgcattgca ggatctgaaa agcaaaccgt tcgccacgtt tttaacggtg
    17341 atggttatcg ccatctccct gacgctgccc agcgtctgtt atatggtgta caaaaacgtt
    17401 aaccaggcgg cgacgcaata ttatccgtca ccgcaaatca ctgtttatct gcaaaaaacg
    17461 ctggacgatg acgccgctgc gggtgtggtg gcacagttgc aggccgagca aggcgtggag
    17521 aaagtgaact atctttctcg tgaagacgcg ctgggcgagt tccgtaactg gtctggtttt
    17581 ggtggtgcgc tggatatgct ggaagaaaac ccgcttccgg cagtggcggt ggtgatcccg
    17641 aaactcgatt ttcaggggac agaatcactg aatacgctgc gtgatcgtat cacccagatc
    17701 aacggcattg acgaagtgcg gatggatgac agctggtttg cccgtctggc ggcgttaacc
    17761 gggctggtcg ggcgcgtttc cgcgatgatc ggcgtgttga tggtggcagc agtgtttctc
    17821 gtcatcggca acagtgtgcg tctgagcatc tttgctcgcc gtgactccat taacgtgcag
    17881 aaactgattg gtgcgacgga tggtttcatc ctgcgcccgt tcctgtacgg tggcgcactg
    17941 ctgggatttt ctggcgcatt gctgtcatta attttgtcag aaattctggt actgcgtttg
    18001 tcatcggcgg ttgcggaagt ggcacaggtt ttcggaacaa agtttgatat caatggctta
    18061 tcgttcgatg aatgcctgct gttgctgctg gtatgctcga tgattggctg ggtggcagcg
    18121 tggcttgcca cggtacaaca tttacgccac tttacgcctg aataataaaa gcgtgttata
    18181 ctctttccct gcaatgggtt ccgtagcagg gaaagagacc ccgttgtctc ttcccggtat
    18241 ttcatctcta tgtcacattt tgtgcgtaat ttattcacag cgttgcattg aacttgtgga
    18301 taaaatcact gtctgataaa acagtgaatg ataacctcgt tgctcttaag ctctggcaca
    18361 gttgttgcta ccactgaagc gccagaagat atcgattgag aggatttgaa tgactgacaa
    18421 aatgcaaagt ttagctttag ccccagttgg caacctggat tcctacatcc gggcagctaa
    18481 cgcgtggccg atgttgtcgg ctgacgagga gcgggcgctg gctgaaaagc tgcattacca
    18541 tggcgatctg gaagcagcta aaacgctgat cctgtctcac ctgcggtttg ttgttcatat
    18601 tgctcgtaat tatgcgggct atggcctgcc acaggcggat ttgattcagg aaggtaacat
    18661 cggcctgatg aaagcagtgc gccgttttaa cccggaagtg ggtgtgcgcc tggtctcctt
    18721 cgccgtccac tggatcaaag cagagatcca cgaatacgtt ctgcgtaact ggcgtatcgt
    18781 caaagttgcg accaccaaag cgcagcgcaa actgttcttc aacctgcgta aaaccaagca
    18841 gcgtctgggc tggtttaacc aggatgaagt tgaaatggtg gcccgtgaac tgggcgtaac
    18901 cagcaaagac gtacgtgaga tggagtcgcg tatggcggca caggacatga cctttgacct
    18961 gtcttccgac gacgattcgg atagccagcc gatggctccg gtgctctatc tgcaggataa
    19021 atcatctaac tttgctgacg gcatcgaaga tgataactgg gaagagcagg cggcaaaccg
    19081 tctgaccgac gcgatgcaag gtctggacga gcgtagccag gatatcatcc gcgcgcgctg
    19141 gctggacgaa gacaacaagt ccacgttgca ggagctggct gaccgttacg gtgtttccgc
    19201 tgagcgtgtg cgccagctgg aaaagaacgc gatgaaaaaa cttcgcgctg ccattgaagc
    19261 gtaatttccg ctactaagca gagaaccctg gatgagaatc cggggttttt gttttttggg
    19321 cctctgcaat aatcagtttc ccctccggca aaacgccaat ccccacgcag attgttaata
    19381 aactgtcaaa atagctattc caatatcata aaaatggggt atgttttagc agagtatgct
    19441 gctaaagcac gggtagtcat gcataaaacg aaataaagtg ctgaatcaca acaccacaac
    19501 acacgtaata accagaagaa tggggaacct caggatgaac atgaagggta aagcgttact
    19561 ggcaggatgt atcgcgctgg cattcagcaa tatggctctg gcagaagata ttaaagtcgc
    19621 ggtcgtgggc gcaatgtccg gtccggtggc ccagtacggt gaccaggagt ttactggtgc
    19681 tgagcaggcg gttgcggata tcaatgctaa aggtggtatc aaaggcaaca aactgcaaat
    19741 cgtgaaatat gacgatgcct gtgacccgaa acaggcggtt gcggtggcga acaaagtcgt
    19801 taacgacggc attaaatatg tgattggtca cctctgttct tcatcaacgc agccagcgtc
    19861 agacatctat gaagatgaag gcattttaat gatcacccca gcggcaaccg cgccagagct
    19921 gaccgctcgt ggctatcagc tgatcctgcg cactaccggc ctggactccg accaggggcc
    19981 gacggcggcg aaatatattc ttgagaaagt gaaaccgcag cgtattgcta tcgtccacga
    20041 caaacagcag tacggtgaag gtctggcgcg tgcggtgcag gacggcctga agaaaggcaa
    20101 tgctaacgtg gtgttctttg atggtatcac cgccggggaa aaagatttct ctacgctggt
    20161 ggcgcgtctg aaaaaagaga atatcgactt cgtttactac ggcggttatc acccggaaat
    20221 ggggcaaatc ctgcgtcagg cacgcgcggc ggggctgaaa actcagttta tggggccgga
    20281 aggcgtggct aacgtctcgc tgtctaacat tgcgggcgaa tcagcggaag ggctgctggt
    20341 gaccaagccg aagaactacg atcaggttcc ggcgaacaaa cccattgttg acgcgatcaa
    20401 agcgaagaaa caggacccaa gcggcgcgtt cgtctggacc acctacgccg cgctgcaatc
    20461 tttgcaggcg ggtctgaatc agtctgacga tccggctgaa atcgccaaat acctgaaagc
    20521 gaactccgtg gatacggtga tggggccgct gacctgggat gagaaaggcg atctgaaagg
    20581 ctttgagttc ggcgtatttg actggcacgc caacggcacg gcgaccgatg cgaagtaatc
    20641 attaatcggc aactttgggt tgccgccaaa ttgctaatat cgagtacgtt gcttcatgcc
    20701 ggatgcggcg taaacgcctt atccggccta caagatccaa agaaatcagt aaattgcaac
    20761 acacgttgta ggcctgataa gcgtagcgca ttaggcaatt tctcgttggt catcactttt
    20821 gaaatcaaac tttacgatta acgcttctcc cagccgccct gttgtgccgt aaatcccagt
    20881 gcctgcataa acgctgtcat cacgccgcga tcttccacgc ctgcatccgc catccaccag
    20941 catgaaacgc caggattgtt acgcaaaacc tcttccagca gatattgccc cacaccgcga
    21001 cggcgggtga cttccctaac acgcagggaa tccagtgccc cctcggtgcc gcttaaggtt
    21061 acccgcacgg ccgcgagaag gcgctcgtta aaacgcgcgg cgtagatacg gtggttatcg
    21121 tcaacctgta aagaggaagg ggaatactcc ggccagatct tttgcaggtc aatccggtcc
    21181 tggtcgctaa agttttctaa tcgaatgatg gtcagcttca tggggaaccc gtgtaaatca
    21241 caaaagtgga accagtgtag cgaaataatt taatcggagg ctttctcttt tttatttctt
    21301 ttggcaggtg attaattttt taacagcaat aattacaaag ttaaaacatt atagaatgaa
    21361 aaatacccag cataatcccc tgaatgatag tgaattattc cgccctttgt gccgttattt
    21421 tatgctgaca acggcacttt tttctgttta tctatcatta aattcagaat attatctgtt
    21481 cttaatcgac tgaaaaatag ggattttaat cgctattatc acaaaatact gcgctaaatc
    21541 cttaatcaga caggcaaaaa cagtgcagta taaaaaaaga acagtctgat ttgttaacac
    21601 ataaaaacaa agcaacacaa catcacgaat ggggattttt gactatgaaa cggaatgcga
    21661 aaactatcat cgcagggatg attgcactgg caatttcaca caccgctatg gctgacgata
    21721 ttaaagtcgc cgttgtcggc gcgatgtctg gcccgattgc ccagtggggc gatatggaat
    21781 ttaacggcgc gcgtcaggcg attaaagaca ttaatgccaa agggggaatt aaaggcgata
    21841 agctggttgg cgtggaatat gacgacgcct gcgacccgaa acaagccgtt gcggtcgcca
    21901 acaaaatcgt taacgacggc attaaatacg ttattggtca tctgtgttct tcctctaccc
    21961 agccagcgtc ggatatctac gaagacgaag gtattctgat gatctcaccg ggagcgacca
    22021 acccggaact gacccaacgc ggttatcaac acatcatgcg tactgccggg ctggactctt
    22081 cccaggggcc aacggcggca aaatacattc ttgagacggt gaagccccag cgcatcgcca
    22141 tcattcacga caaacaacag tatggcgaag ggctggcacg ttcggtgcag gacgggctga
    22201 aagcggctaa cgccaacgtt gtcttcttcg acggtattac cgcgggtgag aaagatttct
    22261 ccgcgctgat cgcccgcctg aaaaaagaaa atatcgactt cgtttactac ggcggttact
    22321 acccggaaat ggggcagatg ctgcgccagg cccgttccgt tggcctgaaa actcagttta
    22381 tggggccgga aggtgtgggt aatgcgtcgt tgtcgaatat tgctggcgat gctgccgaag
    22441 gcatgttggt caccatgcca aaacgctatg accaggaccc ggcaaacaaa ggcatcgttg
    22501 atgcgctgaa agcagacaag aaagatccat ccgggccata tgtctggatc acctacgcgg
    22561 cggtgcaatc tctggcaact gcccttgagc gtaccggcag cgatgagccg ctggcgctgg
    22621 tgaaagattt aaaagctaac ggtgcaaata ccgtgattgg gccgctgaac tgggatgaaa
    22681 aaggcgatct taagggattt gattttggtg tcttccagtg gcacgccgac ggttcatcca
    22741 cggcagccaa gtgatcatcc caccgcccgt aaaatgcggg cgggtttaga aaggttacct
    22801 tatgtctgag cagtttttgt atttcttgca gcagatgttt aacggcgtca cgctgggcag
    22861 tacctacgcg ctgatagcca tcggctacac catggtttat ggcattatcg gcatgatcaa
    22921 cttcgcccac ggcgaggttt atatgattgg cagctacgtc tcatttatga tcatcgccgc
    22981 actgatgatg atgggcattg ataccggctg gctgctggtg gcggcgggat tcgtcggcgc
    23041 aatcgtcatt gccagcgcct acggctggag tattgaacgg gtagcctatc gcccggtgcg
    23101 taactctaag cgcctgattg cgctcatctc ggcaatcggt atgtccatct tcctgcaaaa
    23161 ctacgtcagc ctgaccgaag gttcgcgcga cgtggcgctg cccagcctgt ttaacggtca
    23221 gtgggtggtg ggccatagcg aaaacttctc tgcctctatt accaccatgc aggcggtgat
    23281 ctggattgtt actttcctcg ccatgctggc gctgacgatt ttcattcgct attcccgcat
    23341 ggggcgcgcc tgtcgtgcct gcgcggaaga tctgaaaatg gcgagtctgc ttggcattaa
    23401 caccgaccgg gtgattgcgc tgacctttgt gattggcgcg gcaatggcgg cggtggcggg
    23461 tgtgttgctc ggccagttct acggcgtcat taacccctat atcggcttta tggccgggat
    23521 gaaagccttt accgctgcgg tgctcggtgg gattggcagc attccgggag cgatgattgg
    23581 cgggctgatc ctggggattg cggaggcgct ctcttctgcc tatctgagta cggaatataa
    23641 agatgtggtc tcattcgccc tactgattct ggtgttgctg gtgatgccga ccggtattct
    23701 gggtcgcccg gaggtagaga aagtatgaaa ccgatgcata ttgcaatggc gctgctttct
    23761 gccgcgatgt tctttgtcct cgctggcgtc tttatgggcg tgcaactgga gctggatggc
    23821 accaaactgg tggtcgacac ggcttcagac atccgttggc agtgggtgtt tatcggcacg
    23881 gcggtggtct ttttcttcca gcttttgcga ccggctttcc agaaagggtt gaaaagcatt
    23941 tccggaccga agtttattct gcccgccatt gatggctcca cggtgaagca aaagctgttt
    24001 ctcgtggcgc tgctggtgct tgcggtggcg tggccgttta tggtttcacg tgggacggtg
    24061 gatatcgcca ccctgaccat gatctacatt attctcggtc tcgggctgaa cgtggtggta
    24121 ggtctttctg gtctgctggt gctgggatac ggcggctttt acgccatcgg cgcttacact
    24181 tttgcgctgc tcaatcacta ttacggtttg ggcttctgga cctgtctgcc gattgccggg
    24241 ttaatggcag cggcggcggg cttcctgctc ggcttcccgg tgttgcgttt acgtggcgac
    24301 tatctggcga tcgttaccct cggtttcggc gaaattgtgc gcatattgct gctcaataac
    24361 accgaaatta ccggcggacc gaacggtatc agtcagatcc cgaaaccgac gctcttcgga
    24421 ctcgagttca gccgtaccgc ccgtgaaggc ggctgggaca cgttcagtaa tttctttggc
    24481 ctgaaatacg atccctccga tcgcgttatc ttcctgtacc tggtggcgtt gctgctagtg
    24541 gtgttaagcc tgtttgtcat taaccgcctg ctgcggatgc cgctggggcg tgcgtgggaa
    24601 gcgttgcgtg aagatgaaat cgcttgtcgt tcgctgggct taagcccgcg tcgtatcaag
    24661 ctgactgcct ttaccatcag cgctgcgttt gccggttttg ccggaacgct gtttgctgcg
    24721 cgccagggct ttgtcagccc tgaatccttc acttttgccg aatcggcgtt tgtgctggcg
    24781 atagtggtgc tcggcggtat gggctcgcaa tttgcggtaa ttctggcggc aattttgctg
    24841 gtggtgtcgc gcgaattgat gcgtgatttc aacgaataca gcatgttaat gctcggtggt
    24901 ttgatggtgc tgatgatgat ctggcgtccg cagggcttgc tgcccatgac gcgcccgcaa
    24961 ctcaagctga aaaacggcgc agcaaaagga gagcaggcat gagtcagcca ttattatctg
    25021 ttaacggcct gatgatgcgc ttcggcggcc tgctggcggt gaacaacgtc aatcttgaac
    25081 tgtacccgca ggagatcgtc tcgttaatcg gccctaacgg tgccggaaaa accacggtct
    25141 tcaactgcct gaccggattc tacaaaccta ccggcggcac cattttactg cgcgatcagc
    25201 atctggaagg tttaccgggg cagcaaatcg cccgcatggg cgtggtgcgc accttccagc
    25261 atgtgcgtct gttccgtgaa atgacggtga ttgaaaacct gctggtggca cagcatcagc
    25321 aactgaaaac cgggctgttc tccggcctgt tgaaaacacc ctctttccgt cgcgcccaga
    25381 gtgaagcact cgaccgcgcc gccacctggc ttgagcgtat tggtttgctg gaacacgcca
    25441 accgccaggc gagcaacctg gcctatggcg accagcgtcg tctggagatc gcccgctgca
    25501 tggtgacgca gccggagatt ttaatgctcg acgaaccggc agcaggtctt aacccaaaag
    25561 agacgaaaga gctggatgag ctgattgccg aactgcgtaa ccatcacaac accactatct
    25621 tgttgattga acacgatatg aagctggtga tggggatttc agacagaatt tacgtggtca
    25681 accaggggac gccgctggca aacggtacgc cggagcagat ccgtaataac ccggacgtga
    25741 tccgtgccta tttaggtgag gcataagatg gaaaaagtca tgttgtcctt tgacaaagtc
    25801 agcgcccact acggcaaaat ccaggcgctg catgaggtga gcctgcatat caatcagggc
    25861 gagattgtca ccctcattgg cgcgaacggc gcggggaaaa ccaccttgct cggcacgtta
    25921 tgcggcgacc cacgcgccac cagcgggcga attgtgtttg atgataaaga cattaccgac
    25981 tggcagacgg cgaaaatcat gcgcgaagcg gtggcgattg tcccggaagg gcgtcgcgtt
    26041 ttctcgcgga tgacggtgga agagaacctg gcgatgggcg gcttttttgc cgaacgcgac
    26101 cagttccagg agcgcataaa gtgggtgtat gaactgtttc cacgtctgca tgagcgccgt
    26161 gttcagcggg caggcaccat gtccggcggt gagcagcaga tgctggcgat tggtcgcgcg
    26221 ctgatgagca acccgcgttt gttgttactt gatgagccat cgctcggtct tgcgccgatt
    26281 atcatccagc aaattttcga caccatcgaa caactgcgcg agcaggggat gactatcttc
    26341 ctcgtcgagc agaacgccaa ccaggcgctg aagctcgctg atcgcggcta cgtgctggaa
    26401 aacggccatg ttgtgctctc cgatactggt aatgcgctgc tggcgaacga agcagtgaga
    26461 agtgcgtatt taggcgggta aattatcgat aacccggcag ctaagttctg ccgggttatt
    26521 cgtccgtata cagacttgtt tcaactgacc aggaggatta aaaccagacc acttatttgc
    26581 cacggaaagg ttccctccca tgcattatgc cgttgttgtc atgcttgaac tgctttgctg
    26641 tattcacgct taccgtaccg gacaagagcg gtactggatt tttatcattt tctgttttcc
    26701 ggtgattggc tgcgtggcgt atttcgtcat agtgatgctg cctgaaacgg gcgctgaccg
    26761 tcacgggcat actttgctga tgcgcctgca ggacaaactc aaccctgagc gccacctgcg
    26821 caaattaacc gaagaactgg cgattgccga gaccaatcaa aaccattatg cgttggctaa
    26881 tgaactggcg cgtctggggc gttatcacga agctgttccc cattatcagc aggcgttaag
    26941 cgggatattc gcccatgaag cggcaatgat gctaagcctg gcgcaggcac agtttgctat
    27001 tcaggagttc gccgcctgtc agcagacgct ggaagatgtg atgcgttata accctgattt
    27061 tcagtcggca gacgggcatt tgttatttgc cagaacgtta gccgcacaag aaaaatacgc
    27121 tgatgcggaa agcgagtttg aagtattgat ttcttattac cccggcccgc aggcgcgtat
    27181 ttactatgcg gaaatgttgg cgaaaatgag tcggttacgt gaagccaacg agcagtatgt
    27241 tgccgtggtt gatacggcta aacgctcaag acctcattat agaaaacacc accgggaatg
    27301 gattaagacc gccaatgagc ggctgaagca gagtgtggtg caatgaaaat cttctttgcc
    27361 gttagaccag tgtcgcggca atcttttgtt aaaattgaaa aatgtagctc tcatcgggca
    27421 gcaaaactgg acgcagaatt tgctgccatt tttgctcttc atggtaagga gttctggcga
    27481 ttagctggcc gttaatcgcc tcttttcagt gcattctcgg gatatccgtg ccccatctcc
    27541 cattttccct cacccgtacc gtcaccgcct tgtcatcttt ctgacacctt actatcttac
    27601 aaatgtaaca aaaaagttat ttttctgtaa ttcgagcatg tcatgttacc ccgcgagcat
    27661 aaaacgcgtg aattcgcgca ttcggtacaa caagagagat aaacgatgaa accgttacat
    27721 tatacagctt cagcactggc gctcggactg gcgttaatgg ggaatgcaca ggcagtgacg
    27781 accattccgt tctggcattc tatggaaggg gaactgggta aagaggtgga ttctctggcc
    27841 caacgtttta acgccgaaaa cccggattac aaaattgtac cgacctataa aggcaactac
    27901 gaacagaatt taagcgcggg gattgccgca tttcgtaccg gcaacgcgcc tgctattttg
    27961 caggtttatg aagttggcac cgccaccatg atggcgtcga aagccattaa accggtatat
    28021 gacgtgttta aagaggcggg gattcagttc gatgagtcgc agtttgtgcc gacggtttca
    28081 ggctactact ccgacagcaa aaccggccac ttactctccc agccgttcaa cagctcgact
    28141 cccgttctct attacaacaa agacgccttc aagaaagccg gtttagaccc ggaacagccg
    28201 ccgaaaacct ggcaggatct ggcggactat gccgcaaaac tgaaagcctc cggtatgaag
    28261 tgcggctacg ccagcggctg gcagggctgg atccaactgg aaaactttag cgcctggaac
    28321 ggtctgccgt ttgccagcaa aaacaacggc tttgacggca cagacgcggt gctggagttc
    28381 aacaagccgg agcaggtgaa acacatcgct atgctcgaag agatgaacaa gaagggcgat
    28441 ttcagctacg tcgggcgtaa ggatgaatcc actgagaagt tctataacgg tgattgcgcg
    28501 atgaccaccg cctcttccgg ttctctcgcc aacattcgcg agtacgccaa atttaactac
    28561 ggcgtaggca tgatgcctta cgacgccgat gcgaaagatg cgccacaaaa cgccattatc
    28621 ggcggagcca gcttgtgggt aatgcagggt aaagataaag aaacttatac gggtgtggcg
    28681 aagttcctcg acttcctcgc gaagccagaa aacgctgccg agtggcatca gaaaactggt
    28741 tatctgccaa tcaccaaagc ggcgtatgac ctgacccgtg agcagggttt ttatgagaaa
    28801 aacccagggg cggataccgc gacgcgtcag atgctgaata agccgccgtt accgttcacc
    28861 aaagggctgc gtctgggcaa catgccgcag atccgcgtga ttgtggatga agagctggag
    28921 agcgtgtgga ccggtaagaa gacaccacag caggcgctgg ataccgccgt tgagcgtggg
    28981 aaccagttac tgcgccgctt tgagcagtct acgaagtctt aattctctta ccctcaccct
    29041 aaccctctcc caaagggaga ggggaccgat cggcgtggta tccaggtgcc gtgtcggttt
    29101 gcaacttcgc tcggtgggaa agtatgcacc gcctttctcc ctcgcccctt tggggagagg
    29161 gccggggtga ggggaacaca acacgaagag tttaactatg tcatcatccc gtccggtgtt
    29221 ccgctcgcgc tggctgccct atttgctggt cgcgccgcag ctcattatca ccgttatctt
    29281 ttttatctgg cctgcgggcg aagcgttgtg gtactcgctg caaagcgtcg atccgtttgg
    29341 tttctccagc cagtttgtcg gcctggataa cttcgtcgcg ctgtttcatg acagctacta
    29401 tctcgactcc ttctggacga cgataaaatt cagcaccttt gtcaccgtca gcggtttgct
    29461 ggtgtcgctg ttctttgcgg cgctggtgga gtacatcgtg cgcggcagcc gtttctatca
    29521 aaccttaatg ctgctgcctt acgccgtggc acccgccgtt gccgccgtat tgtggatctt
    29581 cctgtttaac cctggtcgcg ggctgatcac ccattttctc gctgagttcg gctacgactg
    29641 gaaccacgcg caaaacagcg gtcaggcgat gtttctggtg gtgtttgcct cggtgtggaa
    29701 gcaaattagc tacaactttc tgttcttcta tgccgcgctg caatccattc cccattcgtt
    29761 tatcgaagcc gcagccatcg acggtgcagg cccgattcgc cgcttcttta agattgcgct
    29821 gccgcttatc gctccggtga gtttcttcct gctggtggtg aacctggtgt acgccttctt
    29881 cgacaccttc ccggtgatcg acgccgccac gtccggcggg ccggttcagg ccaccacgac
    29941 gctgatttat aagatttacc gcgaaggctt taccggactg gatctggctt cgtctgccgc
    30001 gcagtcggtg gtgttgatgt tcctcgtcat cgtgctgacg gtggtgcagt tccgctatgt
    30061 tgaaagcaag gtgcgttacc aatgattgag aaccgtccgt ggctgacaat attcagccat
    30121 accatgctga tcctcgggat cgcggtgatc ctcttcccgc tgtacgtggc gtttgtcgcg
    30181 gcgacgctgg ataaacaggc cgtctacgcc gcgccgatga cgctaatccc cggcacacat
    30241 ctgctggaaa acatccacaa catctgggtg aacggggtag gcacgaatag tgcgcctttc
    30301 tggcggatgt tgcttaacag ctttgtgatg gcgttcagca ttacgctcgg caaaattacc
    30361 gtctcgatgc tttcggcatt tgccattgtc tggtttcgtt ttccgctacg taacctcttc
    30421 ttctggatga tttttatcac tctgatgttg ccggttgaag tgcgtatttt cccgacggtg
    30481 gaagtgatcg ccaatctgaa aatgctcgac agctacgccg gtttaacgct gccgctgatg
    30541 gcctcggcga ccgccacgtt tcttttccgc cagttcttta tgacgctgcc ggatgagctg
    30601 gtggaagcgg cgcggatcga cggtgcgtca ccaatgcgct tcttttgcga catcgttttt
    30661 ccgctctcca aaactaatct ggcggcgctg tttgtgatca cctttatcta cggctggaac
    30721 cagtatttgt ggccgttgtt gattattacc gatgtggatc tcggcaccac cgtggcaggg
    30781 attaaaggga tgatcgccac tggcgaaggc accacggaat ggaactcggt gatggcggcg
    30841 atgttgttaa cgctgatccc tccggtggtg attgttttag tgatgcagcg tgcctttgtg
    30901 cgcggcctgg tcgatagtga gaaataagat ggcaggactg aaattacagg cagtaaccaa
    30961 aagctgggat ggcaaaaccc aggtcattaa accgctgacc cttgatgtgg cggatggcga
    31021 atttatcgtg atggtcgggc cgtctggctg cgggaaatcg acgttattgc gaatggtggc
    31081 cggactggag cgggtaacga cgggcgatat ctggatcgac cgcaagcgtg tcaccgaaat
    31141 ggagccgaaa gatcgcggga ttgcgatggt gttccagaac tacgcgctct atccgcatat
    31201 gagcgtagag gagaatatgg cgtgggggct gaaaattcgc ggcatgggca agcaacaaat
    31261 tgccgaacgt gttaaagaag cggcgcgcat tctggagctg gacggcctgc ttaagcgccg
    31321 cccgcgtgag ttatctggcg gtcagcgtca gcgtgtggcg atggggcgtg cgattgtgcg
    31381 tgagccggca gtgttcctgt ttgatgagcc gctctctaac ctcgatgcca agttgcgtgt
    31441 gcagatgcgt cttgaactgc aacagctgca ccgtcgcctg aaaacgactt cactctacgt
    31501 tacccacgat caggttgagg cgatgacgct cgcccagcga gtaatggtga tgaacggcgg
    31561 cgttgccgaa cagattggca cgccagttga agtgtacgaa aagcccgcca gcctgtttgt
    31621 ggcgagtttt atcggcagtc cggcgatgaa tctgctggca gggcgtgtga ataacgaagg
    31681 tacgcatttc gaactggacg gcggtatcgc gctgccgcta aacggtggct accgtcagta
    31741 tgctggacgt aaaatgactc tcggcattcg cccggaacat attacgctac gttcgcaggc
    31801 tgaaggtggc gtgccgctgg tgatggacac gctggagatc ctcggcgcag ataacctggc
    31861 gcacggacgc tggggcgaac agaagctggt ggtgcgactg gcgcatcagg agcgcccgac
    31921 ggcaggcagc acgctgtggc tgcatctgcc ggaaaatcag ctgcatcttt ttgatggtga
    31981 aacaggacaa cgagtatgag taactggcct tatccccgca tcgtcgccca tcgtggcggc
    32041 ggtaaactgg ccccggagaa caccctggca gcaatcgacg tcggggcaaa atacggtcat
    32101 aagatgatcg aatttgacgc gaagttatcg aaagatggcg agatcttcct gctccatgac
    32161 gacaatcttg aacgcaccag caacggctgg ggcgtcgcgg gcgatctgaa ctggcaggat
    32221 ttactgcgcg tggatgcagg cagttggtac agcaaagcgt ttaaaggtga gccgctaccg
    32281 ttgctttcgc aggtggcgga acgctgtcgc gaacacggga tgatggcgaa tatcgaaatc
    32341 aaacccacca ctggcaccgg gccattaacg ggcaaaatgg tggcgctggc ggcacgcgaa
    32401 ctgtgggccg atatgacgcc gccgctgctg tcatcgtttg agattgatgc tttagaagct
    32461 gcacaacagg ctgcaccgga actgccgcgc ggtttgttgt tggatgagtg gcgcgacgac
    32521 tggcgagaac tgaccgcgcg actgggctgc gtctctattc atctcaatca taagttgctt
    32581 gataaagcgc gagtgatgca gttgaaagac gccggattac ggattctggt ttataccgtc
    32641 aataaacccc agcgcgcggc agagttgctg cgctggggcg tggattgcat ctgcaccgat
    32701 gcgattgacg tgattggtcc gaactttacg gcccaatagt tttcaacgga atatcaggct
    32761 gcggcgtgct gggctgattc agcatgtcgc cgtttctcct ctccggcaac atatgctgct
    32821 gattcaacgg actatccgga ttacggttgc tgtttaacat cccgccgttg gtgttgggca
    32881 gcatttgctg ccgggcggga tttcgctccc ccggctgtga ctgcaacacc cgctgagaat
    32941 tattgttgat ctggttttct aaatgctgct gttgcaactg cgtttgcgtt ttcagttgct
    33001 gattcagcat ccctttttgc tggatttgct gcgtttgcat ttgagtctgc atccgctgct
    33061 ggctggggat ctgatatccc ggctggttgg ggttgttcag agtattaatg ggctgtgcaa
    33121 agccgacaaa cggcaggagt gccgtcaaaa gcagaagtcg tttcatcgcg tatcctcctc
    33181 tgaagatatt ctttaagttt actcgcttcc cggcaaaacg atgattaatt cagagttata
    33241 taccaggctt agctggggtt gccccttaat ctctggagaa taacgatgat aaaaccgacg
    33301 tttttacgcc gggtagccat tgctgctctg ctctcaggaa gttgttttag cgccgccgcc
    33361 gcgcctcctc cgccacctgt ttcttatggc gtggaggaag atgtcttcca tccggtacgc
    33421 gcgaaacagg ggatggtggc gtctgtagat gctactgcca ctcaggtggg cgtggatatc
    33481 ctcaaggagg gcgggaatgc cgttgatgct gccgtggcgg tgggctacgc gctggcggta
    33541 acgcatccgc aggcggggaa tctgggcggc ggtggtttta tgctgatccg ctcgaaaaat
    33601 ggcaatacca ccgctatcga tttccgcgaa atggcacccg ccaaagcgac ccgcgatatg
    33661 ttccttgatg atcagggtaa cccggacagc aaaaaatcac tcacttcgca tctggcctcc
    33721 ggtacgcccg gtacggtagc aggtttctcg ctggcgttgg ataaatacgg caccatgccg
    33781 ttgaacaaag tggtgcaacc ggcgtttaaa ctggcacgcg atggttttat cgttaacgac
    33841 gcgttggctg acgatctcaa aacctacggt agcgaagtgt tgccgaatca cgaaaacagc
    33901 aaggctatct tctggaaaga gggcgcgccg ctgaaaaagg gcgacaagct ggtgcaggcg
    33961 aacctggcaa agagcctgga gatgattgcc gaaaacggcc cggacgaatt ctacaaaggc
    34021 acgatagcgg aacagattgc ccaggagatg cagaaaaacg gtggcttgat cactaaagaa
    34081 gatttagcgg cgtataaagc ggtcgaacgt acaccgatta gcggcgatta tcgcgggtat
    34141 caggtttact ccatgccacc gccatcttcc ggcgggatcc atatcgtaca aatcctcaat
    34201 attctggaaa acttcgatat gaagaaatac ggctttggca gcgccgacgc gatgcaaatc
    34261 atggcagaag ctgagaaata tgcctacgcc gaccgctcgg aatatcttgg cgacccggat
    34321 tttgtcaaag ttccgtggca ggcgctgacc aataaagcct atgccaaatc tattgccgat
    34381 caaatcgata tcaataaagc gaagccatcc agcgaaattc gccccggcaa gcttgcgcct
    34441 tatgagagta atcaaactac ccattactca gtggtggata aagacggcaa cgcggtagcg
    34501 gtgacctata cgctgaacac caccttcggg acgggcattg tcgcgggcga gagcggtatt
    34561 ctgcttaata accagatgga tgatttctcc gccaaacctg gcgtaccgaa cgtttacggg
    34621 ctggtgggtg gtgacgccaa cgcggtcggg ccgaataaac gcccgctgtc gtcgatgtcc
    34681 ccaaccattg tggtgaaaga cggtaaaacc tggttagtga ctggtagccc aggcggtagc
    34741 cggatcatca ctaccgtgct gcaaatggtg gtgaacagca tcgattatgg tatgaatgtc
    34801 gccgaagcga ccaatgcacc gcgtttccac catcagtggt tgccggacga gctgcgtgtc
    34861 gaaaaagggt ttagcccgga tacgctcaag ctgctggaag caaaaggtca gaaagtggcg
    34921 ctgaaagagg cgatgggtag tacacaaagc attatggttg ggccggacgg cgagttgtac
    34981 ggcgcatccg atccgcgctc ggtggatgat ttaacggcgg gttattaagg ttagcggccc
    35041 tcttcgtggg aagagggccg tattgtcagg gcaagccgaa ggtagccttt tttatttcat
    35101 aaacctgtag atattcttcc agcggctttg ccgttccaag ggaatgaatt tcaccactgt
    35161 ctttatcaat aataaaaggt gcgttaccgg ctaaacgcgc ggcctcatct ccagattcaa
    35221 gaaattcttg tgattcgaaa cagaaatacc agccctggct aaaacgtcca tgcagagtga
    35281 tgacggcagg gagatctgca tcatcaaggt aatggttcgc tttcgcgaat gcgtcgtgat
    35341 aagtaatcat aattataata aatattatta ttgatcgtta tattattatt cttgcgttat
    35401 actttgagaa aagacgcgtt catcttcgtc aaagatatta ttaaatgctg gttttgaaaa
    35461 aagttcatcg gataatatac agtcacgaaa taagtatctg ctaatatttt gtgatttctt
    35521 tttcagaacg tcgaacggga ttttcttatt taagatatca gaatcttatg attcgggtga
    35581 aattctaact aaatcaacat gtcactaaaa tcatcatagc cattagctat ttcggctaaa
    35641 atagagatta catgtcttcg gtccatctca cttaaggagt gtagttccgt tgtaagtttt
    35701 tccatagctt gcattgctaa gtttcgaaca aggaattttc tgctggtaat ctctaaaaag
    35761 atggcatggt ttacaatgat ttttgttat
//
LOCUS       DACBVI010000031        35739 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.031, whole genome shotgun sequence.
ACCESSION   DACBVI010000031 DACBVI010000000
VERSION     DACBVI010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.031"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            192..1847
                     /locus_tag="IU325_07610"
     CDS             192..1847
                     /locus_tag="IU325_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP5808900.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMANAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIQKLPFSKDDVHAVRGGEQEAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDIFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLSAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(1949..3184)
                     /locus_tag="IU325_07615"
     CDS             complement(1949..3184)
                     /locus_tag="IU325_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HAP5808901.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASETQNEVAFTWQGGEPTLLGLDFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSALQPLQVYDFLCDAGAEFIQFIPVVERLADETAAHAGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMVDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(3331..3407)
                     /locus_tag="IU325_07620"
     tRNA            complement(3331..3407)
                     /locus_tag="IU325_07620"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3371..3373),aa:Pro,seq:tgg)
     gene            complement(3450..3536)
                     /locus_tag="IU325_07625"
     tRNA            complement(3450..3536)
                     /locus_tag="IU325_07625"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3500..3502),aa:Leu,seq:cag)
     gene            complement(3557..3632)
                     /locus_tag="IU325_07630"
     tRNA            complement(3557..3632)
                     /locus_tag="IU325_07630"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3597..3599),aa:His,seq:gtg)
     gene            complement(3691..3767)
                     /locus_tag="IU325_07635"
     tRNA            complement(3691..3767)
                     /locus_tag="IU325_07635"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3731..3733),aa:Arg,seq:ccg)
     gene            complement(3870..5255)
                     /locus_tag="IU325_07640"
     CDS             complement(3870..5255)
                     /locus_tag="IU325_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP5808902.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPTAMAKVSRNGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKIFGL
                     NRHGKAHKLEE"
     gene            complement(5446..6186)
                     /gene="rffM"
                     /locus_tag="IU325_07645"
     CDS             complement(5446..6186)
                     /gene="rffM"
                     /locus_tag="IU325_07645"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HAP5808903.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQMLFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(6189..7541)
                     /gene="wzyE"
                     /locus_tag="IU325_07650"
     CDS             complement(6189..7541)
                     /gene="wzyE"
                     /locus_tag="IU325_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen assembly polymerase"
                     /protein_id="HAP5808904.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIHGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(7538..8617)
                     /gene="rffT"
                     /locus_tag="IU325_07655"
                     /gene_synonym="wecF"
     CDS             complement(7538..8617)
                     /gene="rffT"
                     /locus_tag="IU325_07655"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HAP5808905.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSDHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLALFSEENLQILNEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(8614..9864)
                     /gene="wzxE"
                     /locus_tag="IU325_07660"
     CDS             complement(8614..9864)
                     /gene="wzxE"
                     /locus_tag="IU325_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HAP5808906.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(9866..10996)
                     /gene="rffA"
                     /locus_tag="IU325_07665"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(9866..10996)
                     /gene="rffA"
                     /locus_tag="IU325_07665"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HAP5808907.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPVGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(11001..11675)
                     /gene="rffC"
                     /locus_tag="IU325_07670"
                     /gene_synonym="wecD"
     CDS             complement(11001..11675)
                     /gene="rffC"
                     /locus_tag="IU325_07670"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HAP5808908.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVGDSGAEVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGAIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(11653..12534)
                     /gene="rfbA"
                     /locus_tag="IU325_07675"
     CDS             complement(11653..12534)
                     /gene="rfbA"
                     /locus_tag="IU325_07675"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP5808909.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTQGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKLVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(12553..13620)
                     /gene="rffG"
                     /locus_tag="IU325_07680"
     CDS             complement(12553..13620)
                     /gene="rffG"
                     /locus_tag="IU325_07680"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP5808910.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPACVMHLAAESHVDRSIDGPAAFVETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYHDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(13620..14882)
                     /gene="wecC"
                     /locus_tag="IU325_07685"
     CDS             complement(13620..14882)
                     /gene="wecC"
                     /locus_tag="IU325_07685"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HAP5808911.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSDRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(14879..16009)
                     /locus_tag="IU325_07690"
     CDS             complement(14879..16009)
                     /locus_tag="IU325_07690"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HAP5808912.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPNKKMILVTGHRRESF
                     GRGFEEICHALANIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(16065..17111)
                     /gene="wzzE"
                     /locus_tag="IU325_07695"
     CDS             complement(16065..17111)
                     /gene="wzzE"
                     /locus_tag="IU325_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HAP5808913.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIVFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(17123..18226)
                     /gene="wecA"
                     /locus_tag="IU325_07700"
     CDS             complement(17123..18226)
                     /gene="wecA"
                     /locus_tag="IU325_07700"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HAP5808914.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(18454..19713)
                     /gene="rho"
                     /locus_tag="IU325_07705"
     CDS             complement(18454..19713)
                     /gene="rho"
                     /locus_tag="IU325_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP5808915.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(19798..19899)
                     /gene="rhoL"
                     /locus_tag="IU325_07710"
     CDS             complement(19798..19899)
                     /gene="rhoL"
                     /locus_tag="IU325_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide rhoL"
                     /protein_id="HAP5808916.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(20040..20369)
                     /gene="trxA"
                     /locus_tag="IU325_07715"
     CDS             complement(20040..20369)
                     /gene="trxA"
                     /locus_tag="IU325_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HAP5808917.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            20500..21765
                     /gene="rhlB"
                     /locus_tag="IU325_07720"
     CDS             20500..21765
                     /gene="rhlB"
                     /locus_tag="IU325_07720"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HAP5808918.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            21901..23385
                     /gene="gppA"
                     /locus_tag="IU325_07725"
     CDS             21901..23385
                     /gene="gppA"
                     /locus_tag="IU325_07725"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HAP5808919.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLAQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLTVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIDQESQWQSYVHWPLEVH"
     gene            complement(23432..25453)
                     /gene="rep"
                     /locus_tag="IU325_07730"
     CDS             complement(23432..25453)
                     /gene="rep"
                     /locus_tag="IU325_07730"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HAP5808920.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGAELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            25671..26120
                     /locus_tag="IU325_07735"
     CDS             25671..26120
                     /locus_tag="IU325_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808921.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGQFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            26272..26553
                     /locus_tag="IU325_07740"
     CDS             26272..26553
                     /locus_tag="IU325_07740"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase C"
                     /protein_id="HAP5808922.1"
                     /translation="MAKTAAALHILVKEEKLALELLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(26599..28074)
                     /gene="ilvC"
                     /locus_tag="IU325_07745"
     CDS             complement(26599..28074)
                     /gene="ilvC"
                     /locus_tag="IU325_07745"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HAP5808923.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            28224..29114
                     /gene="ilvY"
                     /locus_tag="IU325_07750"
     CDS             28224..29114
                     /gene="ilvY"
                     /locus_tag="IU325_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HAP5808924.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLDNSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(29111..30655)
                     /gene="ilvA"
                     /locus_tag="IU325_07755"
     CDS             complement(29111..30655)
                     /gene="ilvA"
                     /locus_tag="IU325_07755"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HAP5808925.1"
                     /translation="MADSQPLSGTPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(30658..32508)
                     /gene="ilvD"
                     /locus_tag="IU325_07760"
     CDS             complement(30658..32508)
                     /gene="ilvD"
                     /locus_tag="IU325_07760"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HAP5808926.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDMIAIDIPNRGIQLQVSDAELV
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(32573..33502)
                     /gene="ilvE"
                     /locus_tag="IU325_07765"
     CDS             complement(32573..33502)
                     /gene="ilvE"
                     /locus_tag="IU325_07765"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HAP5808927.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(33522..33785)
                     /gene="ilvM"
                     /locus_tag="IU325_07770"
     CDS             complement(33522..33785)
                     /gene="ilvM"
                     /locus_tag="IU325_07770"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HAP5808928.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(33782..35428)
                     /locus_tag="IU325_07775"
     CDS             complement(33782..35428)
                     /locus_tag="IU325_07775"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HAP5808929.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPVTCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQVHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(35431..35481)
                     /locus_tag="IU325_07780"
     CDS             complement(35431..35481)
                     /locus_tag="IU325_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808930.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(35568..35666)
                     /gene="ilvL"
                     /locus_tag="IU325_07785"
     CDS             complement(35568..35666)
                     /gene="ilvL"
                     /locus_tag="IU325_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HAP5808931.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
BASE COUNT         8680 a         9821 c         9052 g         8186 t
ORIGIN      
        1 gccttacata tcacctgcgc cagaggtagg attgaaaacg ctctcctgat tttccaattc
       61 attatctgga taaataaata atttattttc atcactatta tttatgcaat catcctgtca
      121 gggagaggga tctcaattat caatgcttaa ttacgtcaac attttgacga catgaaaccg
      181 gagcattttg catggaattt tcgttttcac ccaaacttct tgttgtggct gtcgccgccg
      241 ctcttccact catggccaac gcagcagata ccccgtcaac cgccaccgca cgcaaaggct
      301 ttgccggata cgatcaccca aaccagtatc tggttaaacc ggcgaccact attgccgaca
      361 acatgatgcc ggtgatgcag catcctgcac aagacaaaga aacccagcag aagctggcag
      421 aacttgagaa aaaaaccggt aagaaaccga acgtggttgt tttcttgctg gatgatgtgg
      481 gctggatgga tgttggcttt aacggcggcg gcgtggcggt gggtaatcct acaccagata
      541 tcgacgccgt tgccagccag gggctgattt taacttcggc gtattctcaa ccgagttctt
      601 ccccaacccg cgccacgatt cttaccggac aatactccat ccaccacggc attctgatgc
      661 cgccaatgta cgggcaaccg ggcggtctgc aagggttaac cactctgccg cagttgctgc
      721 acgatcaggg ctacgtcact caggccatcg ggaaatggca tatgggggaa aacaaagagt
      781 cgcagccgca gaacgttggc tttgatgatt tccgcggctt taactcggta tccgatatgt
      841 ataccgaatg gcgtgatgtt cacgtcaacc cggaagtcgc cctgagcccg gatcgttctg
      901 aatacatcca aaaactgccg ttcagcaaag atgacgttca tgccgtgcgc ggcggtgaac
      961 aggaagccat tgccgacatt acgccgaaat atatggaaga cctggatcaa cgctggatgg
     1021 aatatggcgt taagttcctc gacaagatgg cgaagagcga taagcctttc ttcctctact
     1081 acggcactcg cggctgtcac ttcgataact acccaaatgc gaaatatgcg ggtagctctc
     1141 cggcgcgcac ctcttacggc gactgcatgg tggagatgaa cgatatcttc gccaatctgt
     1201 ataaagcact ggagaaaaac ggtcagcttg ataacacgct gattgtgttc acctctgata
     1261 acggtccgga agccgaagtt ccgccacacg gtcgcacgcc gttccgcggt gcgaaaggtt
     1321 ctacatggga aggcggcgtt cgtgtgccaa ccttcgttta ctggaaaggc atgatccaac
     1381 cgcgtaaatc tgacggcatt gtcgatctgg cggatctctt ccctacggcg ctggatctgg
     1441 cggggcatcc tggggcgaaa gtagcgaatt tagtaccgaa aaccaccttt atcgatggtg
     1501 tggaccagac atctttcttc ctgggaacaa atggtcagtc taaccgtaag gccgagcact
     1561 acttcctcaa cggtaaactt tctgctgtgc gtatggatga gttcaagtat cacgtcctga
     1621 tccagcaacc gtacgcgtat acccagagcg ggtatcaggg tggattcacc ggcacagtaa
     1681 tgcaaacggc gggatcgtcg gtgtttaacc tgtacaccga tccgcaggaa agcgactcca
     1741 tcggcgtgcg ccatatccca atgggtgtac cgctacagac cgaaatgcac gcgtatatgg
     1801 agatcctgaa aaaatatcca ccacgcgcgc agattaaatc tgattaagcc ggcgctatcg
     1861 ctacatcggg ccacactgac cttgccggat gcgacgtgaa cgccttatcc ggcaattaac
     1921 cgcaggcggc cacaaaatgg ccgctttttt acttattcac caccatcaaa tgcgcctgca
     1981 taatgtcgct ggccggacga ccgtgcgcca gcaaatcagc cattgcttta agatatggcg
     2041 gtagatggcg gaaataacgc tgatacccgg cacacaaata attcagcccg ggtttgccgc
     2101 tggcatcgag cataaagcgg tgtttcggac agcctcccca gcacgctttt aacacgttgc
     2161 aactgcgaca ctgcgccggt aactgcttaa atttatcttc accaaacacc tgctgttgcg
     2221 gggaatctac catttctgca attgtttgct ggtgcatatt ccccagtcga tattgcggat
     2281 aaacatagtg atcgcaggcg taaacgtcgc cgttgtgttc aacaatcacc gagcgcccac
     2341 aggttggctg atgatggcaa accgcacctg gcgcaccaac aaaattggca aacgcccatt
     2401 cgatattcat cacgaaaatc ttgccgacgt cgcgtttgat ccagtggtcg aatatcgcca
     2461 ccaaaaattc accgaactca tcggggcgta ccgaccattc cgtcagttcg ccctgaatat
     2521 cgccgggagc atgtagctta agtccagcat gagcagccgt ttcatcagcc aggcgctcga
     2581 ccaccggaat aaactggatg aactcagcgc ctgcatcaca cagaaaatca tagacttgca
     2641 aaggttgtaa cgcactggtg cgattaacgc agaccagcac gttatagtcg acatgatgtt
     2701 tttgcaggag cgtcagggca cgcatcacca gcttatgcgt tggtctgccg cctttggtca
     2761 cgcgatattg attgtggatc tcagccggac catccagcga taaccctaca agaaaatgat
     2821 tttctgccag aaatgcacac cattcgtcat caagcagaac gccgttagtc tggaagctgt
     2881 tacttatctt cctgccagca ccatatttcg cctgtagcgc cacggcacgg cggtaaaaat
     2941 ccagcccgag taacgttggt tcgccgccct gccaggtaaa agccacttcg ttttgggttt
     3001 cgctggcagc gatatagtga cggacatacg cttccagcgt gtcatcgtcc atatgcgtga
     3061 ctggcttttc gcggtaaagg gattgttttt cgagataaaa acagtagtca cagttcagat
     3121 tacaatcgga accgctcggt ttcgccatca catgaaaagc acgcgttgga acctgttgca
     3181 gcatggtcgc tccttgcctg aagtcatcct tcaataagtt aagggcgaaa tcataccgtg
     3241 gcgcaatgag ttattacggg cgtttattgc gaagggaaag agacgaaatg cacagaaaac
     3301 aaaaaacccc gccgaagcgg ggttcaaaat tggtcggcga gagaggattc gaacctccga
     3361 cccactggtc ccaaaccagt tgcgctacca agctgcgcta ctcgccgaaa tactgctttt
     3421 tgaattttta gttcaattct ttaaagtcgt ggtgcgaggg gggggacttg aacccccacg
     3481 tccgtaagga cactaacacc tgaagctagc gcgtctacca attccgccac cttcgcattg
     3541 tcacaacttc taataatggg gtggctaatg ggattcgaac ccacgacaac tggaatcaca
     3601 atccagggct ctaccaactg agctatagcc accactacaa atcttgttac gcggtattac
     3661 taccaccgca gctcaagcgc cgggactaaa tggcgcgccc gacaggattc gaacctgaga
     3721 cctctgcctc cggagggcag cgctctatcc agctgagcta cgggcgctta gcgccgttgc
     3781 gggggtggat aatacggact tcccacccct ctgtctagtc cctttttaaa taaattgcgc
     3841 gtttggttac gatttgtgcg ttttgctgct tattcctcca gtttatgcgc tttaccgtgg
     3901 cgattaagcc caaaaatttt ataaatcgcc gtcaccgcca gcataaagat gatgccgaca
     3961 aacagcgaca tacgcgtatc ttcattaaag tacatgccga tcaaaacgca aatcaggaat
     4021 gccattgtta cgtagttggc ccacgggaac aggattgagc ggaacggatg gctggcaatc
     4081 gccgctttat gcgcacgacg aaaacgcagc tggcttatca atatcacaaa ccatggcacc
     4141 atccccggaa gcacgctggc actgtagacg tagacaaaca cacgctgcgg attgggaatg
     4201 atgtagttca ggcatgagcc aattaacagg atagcaatag atactgccac acccgcaacc
     4261 ggtacgccgt tacgggaaac tttcgccatc gccgtcggta actgacggtt tttcgccagt
     4321 gcgtagagca tacgtccgca actgtacatg ccgctgttac agccagagag cgcagccgtc
     4381 agcaccacaa agttgataat gcccgccgct gcggtaatgc cgattttggc gaaagtcagt
     4441 acgaacgggc taccgttgct gcctatttca ttccacggga agatagtaac gataacgaaa
     4501 atcgcgccta cgtagaaaat cagaatcctc cacagcactt tgcctactgc actgcgcagc
     4561 gtcacctgcg gattcttcgc ttcaccagcg gtaatgccaa tcagctccac gccctggtag
     4621 gacgccacca caatacacaa tgcggtcagg aaccctttcc agccccccgc aaagaaaccg
     4681 ccatgctctg tgaggttgct aaaaccaatc gactgcccgc cattgccaaa gccaaagaaa
     4741 atcacgccca ggccaatgac aatcatcacg ataatcgtgg tgactttgat catcgcgaac
     4801 cagaactcga tttcgccgta caaccgcacc gccgccagat tcgccaacgc caccagcgcc
     4861 accgcgatca atgcgggtat ccactgcgcc atctccggga accagaactg gacataaacg
     4921 ccaatggcgg tgatttctga gatccccacc gccatccaca taaaccagta agaccaggcg
     4981 gtgagatagc caaagaacgg gctcatataa cgatgcgcat aaacggcgaa cgaaccggta
     5041 accggttcga ggaataacat ttcgcccatt gaacgcatga tgaaaaagac gaacagcccg
     5101 gcgatgatat aggccaacaa tacggatggc ccggcccatt tcagggtact ggcggccccc
     5161 ataaacaggc cgacgccaat ggtgcccccc agggcgatga gttcgatatg tcgagcttcc
     5221 agcccacgct gtagctctgg tttgttatct gccataaatc ctcgtgttgt gtttgcatgc
     5281 tttccggtgt taccggttat cgttatgggt acatcgagtg ttgcgaatgt tttcgtaatt
     5341 caggagaaat ggcaaataaa gcattaaaaa tttgaatgct ttgtgtaata aaaaagcagg
     5401 aaggcgacga agtgaccact ccgtcgcttt acagagagag gaaaatcaga ggttgccggt
     5461 gtaatgccag cgtaaataac gcagcaaacg aagctgacgc ttaatgcggc tcggctgcga
     5521 aagcaggcgg tagagccact ccagccccag cgtttgccag attttcggtg cacgttttac
     5581 gtgaccggtg aaaacatcat aagtcccgcc aacgcccata tacagcgcat ctggatgcac
     5641 cagacggcag tcgcgcatga agatctcctg ctttggcgat cccatcgcaa cggtgacgat
     5701 ttgcgctccg ctggcatgaa tgcgttcaaa cagcatctga cgctgctcgg gtttaaaata
     5761 accatcctga ctgccaacga tattcacatt ccactggttg cgtagtttag cttcagtttg
     5821 cgccagcact tcaggtttac cgcccaccag aaataccggc gtcccttctt tgcctgcgcg
     5881 cgccatcagc tcttcccaga gatcggcccc ggcaacgcgg gaaacctgcg cctgcgggta
     5941 ctttttacgt actgaacgta caacgctgat gccatccgca tatttaaatt cggcagcgtt
     6001 aattaactcc ctgacctcgg cgttatcttc aatggtcagc attttttcag cattaatggc
     6061 aaccagcgtt ccctgcttaa gctgcccgtc agcgaacaga taatcgaggg cgtgctgcat
     6121 atcacgccaa ccgatcaact gtaagccacg cagcgtatag gtcggtgccg tggtgttgtt
     6181 attcattgtt atccttcaac ctgtgtccgg agcgatgatt ttgtacgttt atgaatgagt
     6241 ccggcgcttt caaaaagcca gtacagcagt tttgcgatca tcagacatgc gccgaagacc
     6301 acgataaaaa agactacgcg tgagacaaac gaatccagcc cttcacgcgc cagcacgatc
     6361 atattgaaga tcgccccaaa gcagaaactg tgcaatatcg cagctttata gcgattaggc
     6421 tcgcggttgc ccagctcata cagccagtcg aaccatttga tgatcagacc aaccacgatc
     6481 gccccgagcg ggatgaacaa cgcgccgccc atcaccacca gtgagcctat aagcgtaggc
     6541 gagatcgcca gcccggagtg gttattcagc acttcccagg taaagtagtt ggcggagttc
     6601 agtaccatac tcgggcgacc cggccacagc caggaaggga taaagacata gaaatcgcgg
     6661 acaatgggag ccagaccctg gaagtcgatg ttgtcgtagt tctgcaacag caatgccaga
     6721 ttctcccacg gcgaaaaggt atcgcgggtg agatagagaa acgtatagaa cgcttcatca
     6781 ccgctcacat tcattccata gcgttttagc gccagccaga acatgccaac aatccccagc
     6841 acgcccgctg ccgccagcat ccacaacgaa atccagccgt gaataatgcc aataaacagg
     6901 aagatagcga atgcgataat gatattggcg cgagtgccgc cgacaatcat ataagtcagc
     6961 aagccaaaag cgaccgtgct gacgaggaaa aacagccacg ctttgctgtc ctggcgcaga
     7021 aagtagacca ccagcatcgc cgggatgaaa aagtaaaaga agcgttttaa cgccacgccg
     7081 gagacttcac tggagaagat ctgactgtag gagttaagcc ggaacagcaa aaatccgttg
     7141 tgcatgaaga agatgccgac gcttaccagc gcgatgccca tcaggatcac ccacgtgaga
     7201 ttggtctcca cgcggttcat ggtaaacagc ggacggcgcg gtacatcggc aacgcgtttg
     7261 cgtaggcggg ttttgtaggt gacgtagtaa accgcgtaga agcagcccgc agaaagcaac
     7321 gcctgcaaca agatttctgg cggcgcgaca ccaacatcaa agcgaaatac cagcacgctg
     7381 gtcagcggga agccgaagaa aaaggtgagc aaaaacagca atgaaaagaa gacattgaag
     7441 ttaaaacgca cacggcgaaa ctcaaaccag gtcagcgtgg caataaacag cgtgcagagc
     7501 agccagacaa caaacaagcc actgaattgc agcagactca tgcgacctcc ccggcggcaa
     7561 tcgccaacgc ccgctgccag ccttgcagat agttcgggct aaagaaggca atggtgtttt
     7621 tatccaccga cgccaactgg cgctgcgctt cacgcacaat atcctcgttg agatcgtcgg
     7681 tagtaaacag caccggcaaa tgctgttccg tcatatcctg ccagaacgga ttttcccggt
     7741 taagcacaca aggaatgccc gcctgaatca gtaagcacag cgtaccaatg ccctgctggc
     7801 gggcaaaaat aaagtaacca agatcgcact gacgaagtag cgccagatag gcgtcaaatt
     7861 ccagtttttc gttcagaatt tgcagatttt cttcgctgaa taacgctagc cccgcctgac
     7921 gaacttcctc gatgtacgct tcgttattag gcggatatcc catcggcacc accactttta
     7981 ccgtatcgcc aaattgctga tgaacggcgc gcaaggcagc aatatgctca ttgctgcggt
     8041 cgccggagtt gcccaccaga atggtcattt tcccttcacg ttgccgatcg ttcgccatcg
     8101 tattgagcga aggatccatc cgcgtcggga agtacagcag ttcgccccgc acctttgggt
     8161 gcgttttggc aaaaaagctc aaatcaccgc gggtggcaaa tacacagccg actcgctttt
     8221 gcgccaggcg acgtagtggg taaaaaagct tatatctcaa gccactggaa agctcataca
     8281 ggtctgcccc ccagatatgc cagaaaaact ggctgggctt aatgccacca ctcagcagag
     8341 ccagccacag tgtgggattg aactgaccgt ggaaaaaaaa tcgctgctga cggttagctt
     8401 ttgctttcgc gatgaccgct tccgccagcg attttttccc agggaaaaat tgcacagaaa
     8461 gcgccggaca gctatcactt aagccgtcgt ccttgccaac aaccataaac tcgcgcgcgt
     8521 gatcgctcgt cgcggccagc gcgtcgttga aaaaccgcaa aacggttcgg ttatggtgag
     8581 ggatatccga tcccagtacg tgaatcagta cagtcatgcc cgcctacgcc agagtaaaaa
     8641 cacgccacaa caaagagaaa aatagacgat ataggttgcc atataggcct gcgccgcgcc
     8701 cagtgcacca tgcgcaggga ttagccagtg ggcaaatacc atcaataaag tgaactggct
     8761 gacttccgcc agaatataaa accgcagtga cgctttggcg atcaccagat aaccaaagac
     8821 ataagcgccc acttttaaca catcacccac caactgccag gcaaagagat cgcgcatagc
     8881 ggtaaattta ttcgacaaca gcagccagat agcaaaatca cgcagcaacc agacggtaaa
     8941 actcgccgcc gccactgccg gtaagacgaa ttttagcgat ttaaccactt cccgggtgat
     9001 atcgcgcttt tccgttagcc gcgacaacgt gggcagcaaa tatacgctga acgatgccgt
     9061 aataaattgc aggtaggcat cggaaatact gctcacccct tgccagatcc ccacctcatc
     9121 ccagctatac tgcgccgcca gcagtttacg catcatgatg taagcaacag gcaaggtcac
     9181 cgacgtaatc aacgccatga gcgtaaattt gctcaactgc cctgccagac cgttatccca
     9241 gctgggtttc agatagctta acgggatgac accacgtttg atcaacatga tggcggcagg
     9301 aattaccacc agcgcgggaa tcagcgccag acccagcaac gccccttcat aaccgcccaa
     9361 acggtaactg acgtagtacg cgagaacgcc aatcaggctg ccgacaatca gggataacgc
     9421 attacctgcg gcatcgcgaa agcctttcat caacgccagt aacaggttgc cccaggcaat
     9481 ccccatttgc accagcgcca ctaaacgcac cagtccctga tagtcggtat taccaaacaa
     9541 tccctggctg attggcgctg ctgccagcac aaaaaccagc gccattagcg tagagaagcc
     9601 aagtaccatc gctgatgaag tgccgaccac gcggcgcagc tgttggggat tatcatggta
     9661 ctgagcaacg tatttggtta cgccattaaa gatgccagcc ccggcaagca cgccgagcac
     9721 ggtaatcaac tggcggaaat ttgccgccag cccaagcccc gccggaccaa atgacaccgc
     9781 cagcaactta ccgaccagta acccggcacc aatcttgacc agtgtactgg ccgccgtcca
     9841 caaggacgct tttgccaacg acatatcagg agaagtagtt caacaaagtc gcaattaccg
     9901 tacgctgatt gacgggcgac aggttgtaga acagcggcag gcgcagcagg cgctcactct
     9961 ctttggtggt gtagcgatct tcaccgtgga actcaccaaa gcgttccccc gcagggcaac
    10021 cgtgcagcgg aatgtaatgg aacaccgcca tgatttccgc ttctttcaga aagttaatca
    10081 acgcgctccg gtcatcaata tcccgcagtt taatgtagaa catatgcgcg ttctgcacgc
    10141 agccaacggg aatcgacggc agctcgatac gcccggcttt cgccagaggt gctaacgcat
    10201 cgtagtagtt ttgccacagc gccagacgtt gctgattgat acgatctgct gcttccagtt
    10261 gcgcccacag gtacgcagct tgcagatcgg acatcaaata gctggagccg atatcgcgcc
    10321 aggtatattt atcgacctga ccacggaaga actggctgcg gtttgtgcct ttttcacgga
    10381 tgatctcggc tcgttcgatc aacgctttat cgttaatcag cgtcgcgccg ccttcaccgc
    10441 ccgccgtgta gtttttggtt tcatggaagc taaagcagcc aatatgacca atggttccca
    10501 gtgcacgccc tttgtaagtg gacatcacgc cctgagcagc atcttccacc acaaacagat
    10561 tatgcttttt cgccaacgcc ataatggtgt ccatttcgca ggccacaccc gcgtaatgga
    10621 ccggcacgat aacgcgcgtt ttgtcggtga tcgctgcttc aatcaacgtt tcgtcgatgt
    10681 tcatggtgtc cgggcgaaca tccacaaaaa cgatttttgc gccacgcagc acaaaggcat
    10741 tggcagtgga gacaaaggtg tagctcggca tgatcacttc atcgccaggc tggatatcga
    10801 gcagcagcgc cgccatctcc agcgaagcgg tgcaggatgg cgtcagtaac actttggcgc
    10861 tgccaaaacg ttgctccagc cactgctggc agcgacgggt aaaaccgcca tcgccacaca
    10921 gtttgccact acccattgcc gactgcatat agtcgagttc ggttcccacc accggcggtg
    10981 cgttaaatgg aatcatgtga tcacctgtat aaccagtacg cggtgctttc tacattcgca
    11041 ccactttgta tgtatcgttt tagcgcggcg gtgttgccca tttgggtcgc cacccgcaaa
    11101 gttgttttac cgcgagcata cgcccagttt agcgccgttt gcatcagctc agcacctgca
    11161 ccgcgtccag ccagcaggcc aattcgcgca tctgtcgcat tgagttcccg taaagagaca
    11221 tagccgcgaa tagcgccgga cgccgcacgt aaaatcagac attgatgatc aaaggtgccg
    11281 cgcacggcat tttcaatcca ctgtgcataa aagcgaccgc tggcgtcagg cgcataccac
    11341 ggcgcacgaa aacggctttg cgcaaatgcg gcgctggcta actgacgtaa tgcgggaata
    11401 tctgtctctt gtgccacttc tgcgccgcta tcaccgacat tgttcacggg tagcgccaaa
    11461 tcaacttcac cttctaccag ggagaatccc agctgttgca gggcatccag ttcacccgta
    11521 tttgatgccg caattttggc ctgcacccgt gaccacggcg ctaacgcgtc tggcgtcagg
    11581 agcggtgctt cagacgtgat gcgcacgatg gcgctgttaa caccaaagaa ggcgttttcc
    11641 caggttagtg gctcaatact ggcgcggacg ggcacgaagt aactccagca gatattggcc
    11701 gtagccagtt ttcgccagcg agctggcggc gcgcttcacg ccttcgtcat caagccagcc
    11761 gttacgccag gcgatctctt ccaggcaggc aatcttaaag ccctggcgtt tttccaccgt
    11821 ctgtacaaag gtgctggctt caatcaggct gtcgtgagtg ccagtatcca gccaggcaaa
    11881 tccgcgcccg agcagttcaa cggtcaggtt gcccgcctcg aggtacatct ggttgatgga
    11941 ggtaatctcc agttcaccac gctccgacgg cttcaccagc tttgcgtact ccacgacttt
    12001 actgtcgtag aaataaagcc cggtcaccgc ccagtttgac ttcggctgtt ttggcttttc
    12061 ttccagcgag atagcgcgga aattgtcgtc aaactccacg acgccaaagc gttccggatc
    12121 catcacctga tagccaaata ccgtcgcgcc ttgcgtgcgc gccgcaacat gacgcaactt
    12181 cggactgaag ccctgtccga agaagatgtt atcgcccagc accagacaag aaggttcacc
    12241 gttgaggaag gtttcaccga tgataaaggc ctgcgccaga ccgtccggac tgggctgttc
    12301 ggcatattcc agctggatac cgaactcact accatcgccc agcaggcgct ggaaataacc
    12361 tttatcttcc ggcgtagtga tgatgagaat ttcgcggata ccggccagca tcagcaccga
    12421 cagcggatag taaatcattg gcttatcgta aatcggcaac agttgcttcg atacgccgcg
    12481 cgtaatcgga tgcaatcggg tgccggaacc gcccgccagg ataatacctt tcatgtttgc
    12541 ctccgctgaa aattagcctt tcagacctaa acgctcgccc tgatagctgc cgtcctgcac
    12601 ctgcttccac cagctttcat tagccagata ccactgcacc gttttacgca ttccactttc
    12661 aaaggtttcc tgcggcagcc agccaagttc acgggcaatt ttcgaagcat caatggcata
    12721 acgcagatca tgccccggac ggtcagcgac aaaggtgatc aggtcatgat agtgcgccac
    12781 gccgtgcggc ttgttcggag ccagttcttc cagcagctcg caaatggttt ccacaacatc
    12841 gagattctta cgctcgttgt gaccaccaat attataggtt tcaccgactt tcccggtggt
    12901 cgccacgcaa tacagcgcgc gggcgtgatc ttccacatac agccagtcac ggatttgctg
    12961 cccgttgcca tataccggca gcggtttacc cgccagcgcg ttgaggatca tcagcgggat
    13021 cagtttttcc ggaaagtggt aaggaccgta gttattcgag cagttggtga taagcgtcgg
    13081 cagaccatag gtccgcagcc aggcgcgcac caggtggtcg ctgctggctt ttgaagcgga
    13141 ataggggctg ctcggcgcat acggcgtggt ttcggtgaag aaatcatccg tcgagtgcag
    13201 gtcaccatat acttcgtcag tggagatgtg atgaaaacgg aacgctgatt ttttatcttc
    13261 cgtcagcgca ttccagtaag cccgcgccgc ttcaagcaat gtataagtcc cgacaatgtt
    13321 ggtttcaaca aacgctgccg ggccgtcaat agaacggtca acatggcttt cggctgccag
    13381 atgcatgaca caggctggct gatgctcagt gaatacgcgc gccagttctg cccgatcgca
    13441 gatatcaact ttctcaaagg caaagcgctc gctttgcgcg accggtgcca gcgacatcag
    13501 gtttccggcg taggtcagct tatcgaccac taccaccgca tcgctcgttt cgttgatgat
    13561 ataacgcacc agcgccgagc caataaaccc ggcaccacct gttatcagaa tctttctcat
    13621 cagcgccaga ctcctttggc atcgacgaca tactgctgat ggacgttgtc gccattgata
    13681 actttgaact gactatgatc gaccagcatc accagcacat ctgccgttgc cagcgcctcg
    13741 tcaagctgcg ccagagtaca aagcccggtc agtttcttcg gcaactggtg gatgttaggc
    13801 tcaacaacca gcgtttcgcc gctgtgccac tgggcgatca gttcagcgat ttccatcgcc
    13861 gggctttcgc gcaggtcatc aatattcggt ttaaacgcca gaccaaagca ggcgattttc
    13921 agttcactgg cgcgtttatc ggtagccgcc agacaatcag ccaccgccgc tttcacctga
    13981 tcgataaccc agaacggttt gtgatcgttc acttcgcgcg cggtacggat aagccgcgcc
    14041 tgctgcgggt tctgtgccac aataaaccac ggatcaacag cgatgcagtg accgcccacg
    14101 ccagggccag gctgaagaat attgacgcga gggtgacgat tcgccaggcg aatcagttcc
    14161 cagacgttaa tcccctgatc ggcacaaatc agcgacaatt cattagcaaa agcgatattc
    14221 acatcgcgga agctgttttc ggtgagctta cacatttccg ccgtccgcga gttagtgacg
    14281 acacactcac cttcgaggaa aatcttgtac agttcgctgg cgcggtccga gcaaaccggc
    14341 gtcatgccgc caatcacgcg atcgttttta atcagctcga ccattacctg tcccggcaac
    14401 acgcgttccg ggcagtaggc aatattgacg tcagcctgct cgcccacctg ctgtgggaaa
    14461 gtgagatctg gacgcatctc tgctaaccat tctgccatct tctcggttga ccccaccggc
    14521 gaggtggatt caaggatcac caacgcgcct tttttcagca ctggcgcaat ggagcgagca
    14581 gccgattcaa cgtaggtcat atctggctca tgatcgcctt taaacggcgt gggtacagca
    14641 atcagccagg catccgcttc aactggcgtc gtgctcgctc gtaaaaaacc gccttctacg
    14701 gcagttttta cgacgctcgc caaatcaggc tcgacgatat ggatttcgcc acgattgatg
    14761 gtatcaaccg cgtgttggtt gatatcgaca ccaatcacct gtttttgccg tgaggcaaac
    14821 gcagctgctg taggcagccc gatgtaaccc agtccgataa cagaaatggt cgcaaaactc
    14881 atagtgatat ccgattattt tttaacgctt ccagaatgcg agagcatgcc tgaccatcac
    14941 catacgggtt atgggcgcgg ctcatagctt gatattcgtt ttcgtctttt aaaagacgcg
    15001 tcacttcctc gacgattcgc tgcttatccg tgccgaccag acgtaccgta cccgccgtca
    15061 ccgcttccgg acgctcagtg gtatcgcgca ttaccagcac cggtttcccc agcgaaggcg
    15121 cttcttcctg aatgccgcct gagtcggtca aaatcagcca ggcgtggttc atcagccaga
    15181 caaacggtaa atactcctgg ggatcgatca gaatgacatt tttcacatgc cccagaatgc
    15241 gattgaccgg ctctctgacg ttcgggttga gatgcactgg atagacaatc tggatgtcct
    15301 ggtgcgtggt ggcgatgttt gccagcgcat ggcagatttc ttcaaagcca cgaccgaagc
    15361 tctcacgcct gtgaccggtc accagaatca tttttttatt ggggtcgata aacgggtaat
    15421 ttgccgccag ttctgaacgc agcgtgtcgc tgctcatcac ctggtcacgc acccataaca
    15481 gtgcatcaat aactgtatta ccggtaatga agattcggct gtcagcaaca ttttcacgca
    15541 gcaagttttg ccgggaagtt tcggttggag agaagtgata catcgccaga tgcccggtca
    15601 atgtacggtt agcctcttcc ggccacggcg aatagagatc gcccgtgcgc agaccagcct
    15661 caacgtgacc aacaggaata cgctgataaa acgccgccag gctggttgcc agcgttgtag
    15721 tagtatcgcc gtgaaccagc acgacgtctg gtttgaactc ggctagaatt ggttttagcc
    15781 cttccagaat ccgacaggtt atctctgtca ggccctgtcc tggctgcatt atgttgagat
    15841 cgtagtcagg tacaatggaa aagagtttca gcacctgatc gagcatctcc cgatgctgcg
    15901 cagtgacgca aactttagcc tcaaaaaaag gatcttttgc caacgcatgc accaacggag
    15961 ccatcttgat ggcttccggg cgcgtaccaa atacagtcag tactttcaca tcgattctct
    16021 tcgaataagc ggcgagcgcc tttgcgctca ccgcagcagt gttgctattt cgagcaacgg
    16081 cgggttaatg cgacaccagc cccgatcagc cccccgacaa tgccccacat aatcatcagg
    16141 aaggcacgac gtgggctatc gcgttttacc ggttcttccg gcgtacgcaa atagcgatag
    16201 gtctgaaaac gcggatccag ggttggacca acattcaggg tgtttaacat ggcccgattc
    16261 tgatcatagt cgagatcaaa ggccggaccg acggcctgta aattttccag tcgagcctgg
    16321 agcattggac gcccaagcag gaacatttct gaatcaggta attcctcggc aggcacatct
    16381 gtcgcactgc gcgaaatatt atgctgctca gcaattttca gcgcctgttc aatactgttc
    16441 atccggcggt cgtagatggc tttcgccacc tcttcctgac gcttcacctg tgctttcatc
    16501 tggatggtac gcgccgccca tgcgcctttc agctcatcat tcagatggct ggctgcacgc
    16561 tggctggcaa aagcaacata ctgacgtaac aggttattag cgtcaggcgc ggtttcggca
    16621 atcagcttaa cgctgtcatt aaccgcgcgg gtgaagtcac cgggaataaa cacgatgttg
    16681 ttaatcattt catccagcaa cgccgcatcg gctttactgt tgcccaccat ccgctgcttg
    16741 taatagtcgg tttgcagcca gaactctctg cgggtatccc acgaggccag ttgcataaca
    16801 aactctttat aggcttcgtc catgaccgat ggttggtcgg cagaagccat gtttgaacgg
    16861 acatccaggt tacgcaaaaa ttgctgctgt gagtaatatc cccccagcat attcaccgtt
    16921 ggacgatcgg taatcgccgt cgagctccac tcctgacgag caaaaaacgt atacgccagc
    16981 gcgattaacg caaacgccag ccccatgcca ataatccata gcttcccagc ccacaaggta
    17041 cgaaacaacc cacgaatatc cagttcattt tcagcgtctt cggccggttt cccaggcatt
    17101 ggttgtgtca tcacatcctc atttatttgg ttaaattggg gctgccacca cgatttctac
    17161 gcagtctgcg ttttacgcgc ttaataaagc gagcaacttt ccaggcacgc ttaatgcaat
    17221 atccatagag gaagaatgct agcaaaaaga gcaccagcat gacccactcc gggacaaaat
    17281 gagaatattc tgccagcacc ccaatggagg cgagcagagc tgcggcaagg gtaatcagca
    17341 caaacgcctg gcgggaagta aacccggcac gcatgatcaa atggtgaata tgctgacggt
    17401 caggagagaa tgggctcatg cctttacgca ggcgacggta cataatcgcc accatatcca
    17461 ttagcggaat ggcgattatc cacaaagcgg taaccgggct gatgggatgg gttttgccct
    17521 gggtcgtttc gagcaggatc cagataacgg taaaaccaat cagcgtactg cccgcatcac
    17581 ccataaagac tttgtagcgg cgacccagga taccaaggtt aagcatgatg tatggcagga
    17641 tggcggcgat catcgcgaag caccagattg cgaggctggt ttgcccgtcg aaccacaaaa
    17701 tcataccgat tgctgcaaac gagacgcagg acaacccgcc cagcaagcca tcaatgccat
    17761 caaccatgtt gaacgcatta atggccgccc agacggcaaa tagcgtcagg aagtagccaa
    17821 acggtccgag caccatctcc caggagccaa agatataacc caggctactg agataaagtt
    17881 tgccgaacac catcataaca atgccaacag cggcctgtat ggtggcacgg atttttacgc
    17941 tgatatcaaa acggtcatcc agcgcgccaa tgaaaacaag cacaccggca caagcgagat
    18001 agagagatgc atgcggaata tagtaatcga caattccgaa cgtgaagcaa atccctgcgt
    18061 aaaccgaaat ccccccaacg agaggtatca atccctggtg acgtttgcgg aagttaggtt
    18121 tatccactaa accgactttt tttgccacct tacgggcaaa aaacagaaac agtgtcgtga
    18181 ataaaaaaat actgatgaga tcagtactca ctgtcagtaa attcacaatg catgctctca
    18241 gagaaaatta ttagcagaag tataaccacg aagaccatta ttcagaaggg aaactctgct
    18301 ctgaacccac caaccttgtg caaaaaacaa ccacgtgatt cgtgttatgc gcaataaaca
    18361 aacataactc gtatttgtta taacgccgtt aagattacag atataaaaac aaaacgccac
    18421 gtaaacacgt ggcgtttttg gcataagaca aatttatgag cgtttcatca tttcgaagaa
    18481 atcgtcattg gtcttggtca ttgccagttt attaatgagg aattccattg catcgatttc
    18541 gcccatcggg tgaatgattt tgcgcaggat ccacattttc tgcagttctt cctgagtagt
    18601 gagcagctct tctttacggg taccggaacg gttgtagtcg attgccggga agacgcgttt
    18661 ttcagcgatc ttacgagaga ggtgcagttc catgttgcct gtacctttaa actcttcgta
    18721 gataacttcg tccatcttgg aaccggtatc gataagcgcg gtagcgatga tggtcaggct
    18781 gccgccctct tccacgttac gcgccgcacc gaagaagcgt ttcggacgat gcagggcgtt
    18841 ggcatccaca ccaccggtca gcactttacc tgacgccgga acaacggtgt tgtaagcgcg
    18901 cgccagacga gtgatggagt cgagcaggat gataacgtct ttcttgtgct caaccaggcg
    18961 tttcgccttc tcgatcacca tttccgcaac ctgaacgtgg cgagatgcgg gctcatcaaa
    19021 ggtggaagca acaacttcac cttttaccag acgctgcatc tcggttactt cttccggacg
    19081 ttcgtcgatc agcagaacca tcaacacaca atccgggtgg ttgtaagcaa tgctctgagc
    19141 aatgttctgc agcagcatgg ttttaccggc tttcggcggt gccacaatca gaccacgctg
    19201 accacgaccg ataggtgatg ccagatccag tacgcgagca gttaaatctt cagtagaacc
    19261 gttaccacgt tccatacgca gacgagagtt tgcgtgcagc ggggttaagt tctcaaagag
    19321 gattttgttg cgggcgtttt caggtttgtc gaagttaact tcgttaactt tcagcagcgc
    19381 aaaatagcgt tcaccttctt tcggcgggcg aatcttacca gagatggtat caccagtgcg
    19441 gaggttgaaa cggcggattt ggctagggga aacgtagatg tcatcaggac cggcgaggta
    19501 ggagctgtct gcggaacgga gaaaaccaaa tccatcctgc aatatctcca gtacgccatc
    19561 accaaagata tcttcgccac tctttgcgtg ctgcttcagg atggcaaaaa taatgtcctg
    19621 cttacgcata cgagccaggt tttccagccc catattttcg ccgagagtga tcagctcaga
    19681 aaccggcgta ttctttaatt cggtaagatt catagtggtg tgagttctta aacttggggt
    19741 aaatctcgaa cttaatgttg tgaatggtat ggcaggatca tccatgcctg tttacggtca
    19801 tcgactcatg tcttttcgct gcctggtcac aggagagtac gcagaactga aacgacaaga
    19861 cggattgagt gacgagccag aaatctgttc acttcgcatt taagataaaa cgggaagcgt
    19921 tggaaattac aagattcaaa cttaataagg tatgtttaat acgaagtcaa cactaagtta
    19981 gcatgactca cgccgggcgt ccagttttta gcgacggggc acccgaacat gaaattccct
    20041 tacgccaggt tagcgtcgag gaactctttc aactgacctt tagacagtgc gcccactttg
    20101 gttgccgcca cttcaccgtt tttgaacagc agcagagtcg ggataccacg gatgccatat
    20161 ttcggcgcag tgccagggtt ttgatcgatg ttcagttttg caacggtcag tttgccctga
    20221 tattcgtcag cgatttcatc cagaatcggg gcgatcattt tgcacggacc gcaccactct
    20281 gcccagaaat cgacgaggat cgccccgtcc gctttgagta catccgtgtc aaaactgtcg
    20341 tcagtcaggt gaataatttt atcgctcata tataactcca caggaataag cctggcgtgt
    20401 tggtttcgtt gttggtgtaa cattaaccaa ctaaaggttg actttatttc accggatacg
    20461 ctttcgtaaa gcaatagtaa gctgatattc taccacacta tgagcaaaac acatttaaca
    20521 gaacagaagt tttccgactt cgcgctgcat ccgaaggttg tagaagccct tgaaaaaaaa
    20581 gggtttcata actgtacgcc cattcaggca ctggcccttc cgctgacgct ggcgggtcgt
    20641 gacgtagccg ggcaggcgca aaccggtacc gggaaaacga tggcgtttct tacgtcaacg
    20701 tttcattatc ttctctctca tcccgcgatt gccgatcgca aggtgaatca accgcgtgcc
    20761 ttaattatgg caccgacgcg tgaacttgcc gtgcagatcc acgccgacgc ggaaccgctg
    20821 gcggaagcta ccggcctgaa gctgggtctg gcttacggtg gtgatggcta cgacaaacag
    20881 ctgaaagtgc tggaaagcgg cgttgacatt ctgattggca ccacggggcg tttaattgac
    20941 tacgccaagc agaaccacat taacctcggt gccattcagg tagtggtact ggacgaagcc
    21001 gatcgtatgt acgatctggg ctttattaaa gatatccgct ggctgttccg ccgtatgccg
    21061 cctgcaaacc agcgcctcaa catgctgttc tccgccacgc tttcgtaccg ggtacgtgaa
    21121 ctggcgttcg agcagatgaa caatgccgaa tatattgaag tggaaccgga acagaaaacg
    21181 ggccaccgta ttaaagaaga gcttttctac ccttctaacg aagaaaaaat gcgtttgctg
    21241 caaacgctga tcgaagaaga gtggccagac agagcgatta ttttcgccaa caccaaacac
    21301 cgttgtgaag agatctgggg ccacctggcg gcagatggtc atcgtgtcgg tttgttgaca
    21361 ggtgatgtcg cgcagaaaaa acgtctgcgt attcttgatg aatttacccg tggggatctg
    21421 gatattctgg ttgccaccga cgttgccgcg cgtggcctgc atatcccggc tgtgacgcac
    21481 gtctttaact acgatttacc cgatgactgt gaagattacg ttcaccgtat tggtcgtaca
    21541 ggtcgtgcag gcgcaagtgg tcattccatc agcctggcat gtgaagagta tgcattgaat
    21601 ttgcctgcta ttgagaccta tattggtcac tcaattccgg taagcaaata caatccggac
    21661 gcattgatga ccgatctgcc taaaccgctg cgcctcacgc gcccgcgtac aggcaatggt
    21721 ccgcgtcgta ctggcgctcc gcgtaatcgt cgtcgttcag gttaaataaa aaatgtcata
    21781 ctcaaaatca ttcaaactgt atcaagatgg caagagaatg aaccccgatg agcttactgt
    21841 agtaagtgat tcaggtgaaa gaatgcagcc aacacagaga cagattgaag gatgaagagt
    21901 atgggttcca cctcgtcgct gtatgcagcc attgatctcg gttcgaatag ttttcatatg
    21961 ctggttgtgc gcgaggtggc tggaagcatc cagacgctga cgcgaattaa acgcaaagtg
    22021 cgtctggctg ctggcctgaa cagcgaaaat gccctgtcta atgaagcaat ggagcgcggt
    22081 tggcaatgtt tgcgcctgtt tgctgaacgt ctgcaagata tccctccctc gcaaattcgc
    22141 gttgtcgcta cggcgacatt acgactagcc gtcaatgcgg gtgattttat cgccaaagca
    22201 caggaaatcc ttggctgccc ggtacaagtc atcagcggtg aagaagaagc ccgcctgatt
    22261 tatcaggggg tggcccacac aacaggcggt gccgatcagc gcctggtggt ggatataggc
    22321 ggtgccagta ctgaactggt aaccggcacg ggtgcacaaa ccacctcgtt gttcagcctg
    22381 tcgatgggct gcgtcacctg gctggaacgc tattttgccg atcgtaatct ggcgcaggaa
    22441 aattttgatg ctgcagaaaa agcggcacgc gaagtgttac gtccggttgc cgatgaatta
    22501 cggtatcacg gctggaaagt gtgcgttggc gcttccggca ccgtgcaggc gttacaggaa
    22561 atcatgatgg cacaggggat ggatgaacgc attaccctgg aaaagttgca gcaactgaaa
    22621 caacgcgcca ttcattgcgg tcggctggaa gagctagaga ttgacgggct gacgctggaa
    22681 cgtgcgttag tgttcccgag tggtctggcg atcctgatag ccatttttac cgaactgaat
    22741 attcagtgta tgaccctggc gggcggtgcg ctgcgtgaag gcctggtcta cggtatgttg
    22801 catcttaccg tcgagcagga tattcgcagc cgtacgctgc gtaatattca gcgccgcttt
    22861 atgatcgata ttgatcaggc acagcgcgta gccaaagttg cggctaactt cttcgatcag
    22921 gtggaaaatg aatggcatct tgaagcaata agccgcgatt tgctcatcag cgcctgccag
    22981 cttcatgaaa tcggcctgag cgttgacttc aaacaagcgc cgcaacacgc tgcttatctg
    23041 gtacgtaatc tggatcttcc cggttttacc cccgcacaga aaaaattgct ggcgacgcta
    23101 ctgctcaacc agacgaatcc ggtcgatctc tcatcgctgc atcagcaaaa tgccgtaccg
    23161 ccgcgcgtcg cagaacaact ctgccgttta ctccgcctgg ccatcatttt tgccagccgt
    23221 cgtcgtgacg atctcgtgcc agagatgaca ttacaggcta accatgaact gttgaccttg
    23281 acgcttccgc aaggttggtt aacccaacat ccgctgggta aagagattat tgatcaggaa
    23341 agccagtggc agagctatgt ccactggccg ctggaagtgc attaatgagt aagtgtcgga
    23401 tgcgatgctg acgcatcttt tccggccttg attatttccc tcgttttgcc gccatcatcg
    23461 ctttcagatt cgccagatgg ctttgccctt tctgcatccg ttcttcggcg ctgaccactt
    23521 tacgctcctg ttcccaaatc aaatcatcct gcggcagctc caataagaat cggcttggtt
    23581 ccgggcgcac cagttcgccg tactgacggc gttctttaca cagcgtaaag gtcaattcct
    23641 tctgggcacg agtaatgccg acataggcca gccgccgctc ctcatcgata ttatcttcat
    23701 cgatgctgct ctggtgcggc aaaaaaccct cttccatacc gaccatgtag acataaggaa
    23761 actccagccc tttcgacgcg tgcagagtca tcagttgtac ctgatccagc tcttcctcgc
    23821 tctcgccgcg ctccatcatg tcgcgcaaag taaagcgcgt caccacctgg gtgagcgtca
    23881 tcggctcatc cagttcgctg ccttccagca tctccgtcat ccagctaaac agttggttga
    23941 cgttcttcat gcgcatttcg gcagctttcg ggctgggcga tgtttcgtac agccaggatt
    24001 cataatccat gccgtggatc agatcacgca ccgcggcaat cggctcccgc tccgccaggc
    24061 gctggatttc tgccaaccag tgagtgaagc gggtcaatgc ttcataacca cgtccgctaa
    24121 gcgtctgact caggcccata tcaaagctgg cggtaaacat gcttttattg cgcgtcatcg
    24181 cccactcacc cagctttttc agcgtcgccg ggccaatctc tcgcttcggc gtgttaacga
    24241 tacgcagaaa tgcgctgtca tcgtccgggt tagtcagcac gcgcagataa gccagcaagt
    24301 ccttgatttc aggacgagag aaaaacgacg taccaccaga tattttgtac gggatgcggt
    24361 tttgcatcag gaatttttca aatacccgcg actgatggtt gccgcgataa agaatggcat
    24421 aatctttgta ctgcgtttta ttgacgaagt gatgcgcgat cagctcgccg gtgactctct
    24481 cggcttcatg ttcttcgtta ttcgcgctta aaacttttag ctccgcgcca tagcccagtt
    24541 cggagaacag acgcttttca aagacgtgcg ggttattggc aatgaggatg ttcgccgctt
    24601 tcagaatacg tccggaagag cggtagttct gctcaagctt aatcaccttc agtgccggga
    24661 aatcctgact cagcagcacc aggttttgcg gacgtgcacc gcgccaggag tagatcgact
    24721 ggtcgtcatc gccaactacg gtaaagcgcg cgcggctgcc caccagcagt ttcaccagtt
    24781 catactggct ggtgttggtg tcctgatact catccaccag caggtagcga attttgttct
    24841 gccagcgctc gcggacttct tcattgcgtt gcagcagtaa cgtcggcaat aaaatcagat
    24901 catcgaaatc gagaacgtta caggctttca ggtgtgcatc atacagccca taacaatggg
    24961 caaaaatacg gtcccgctcg ccaatcgcac tggctgccgc ctgggacggt gttttgagat
    25021 cgttcttcca gttagagatg gtcgaaatca gctgttgcag gaggactttg tcatcttcaa
    25081 tcagcccctc ggtcagctct ttaagcaaag caagctgatc ggtatcgtca aacaacgaga
    25141 agtttgcttt catcccaagc gccgcatact cgcgtttgat gatatccagc cccagtgtgt
    25201 ggaaggtgga gatcatcagc ccacgcgcct ctttgcgacc caacgtctgc ccgacacgct
    25261 ctttcatctc gcgcgccgcc ttattggtaa aggtcaccgc cgcgatatgt cgcgcctgat
    25321 aaccgcaacc gcggatcaga tgggcgattt tattggtgat gacacgagtt ttaccggaac
    25381 ccgcgcctgc cagcaccagg caggggccgg taacgaattc gacagcttgt tgttggccgg
    25441 ggtttagacg cataggtgta ttgctcaatc ttcgaacggg ggggaggatt gtagcagaat
    25501 cgcatttgaa tgtgaccgag tgaaaaccgc taaccggatg gctgcggatg tttaaatcag
    25561 agagggaagg ccgaaaccgg agttcatcat gaatccatta gacttagaaa atatcgggtc
    25621 gtaacgttgt gttattactt tttcagagtt tactaaggag aggtttactc gtggcgatta
    25681 atttcagccc caaggttggt gaaatactgg aatgcaactt tggtaactat cctgttagcc
    25741 agaatggaca gttcagcacg acgtattacg atggccgcat tccacctgag atgataaaaa
    25801 ataggttggt tgtcgtgctc aacggaaaaa ttaatggcaa cgcatgtatc gtcgttccat
    25861 tatcaaccac tcgcgaccat gacaagttaa atcgtggaat gcatgttgaa atagccagta
    25921 atgttatcaa tgacctgcaa ttctttgatc agcagatacg ttgggctaaa gccgacctgg
    25981 tacaacaagt cagtcgaaac cgattaaaca gagcaaggac ttatcggggg tatcttaatc
    26041 aatgtttgcc acatgagttg gttgcagaca tacagagggc agtcatcaag tcaatcaatg
    26101 ccatatcgct cataaattga caataaaatc accatacccg ataatgactc tgtagcccat
    26161 agaggcccta agttttctcc ccttgaggga cctgcgaagc ccggattttc cgggcttttg
    26221 ccatatatgt cccccctccc tgactgacaa tgtttaattc aaggcacgat catggcaaaa
    26281 acagcagcag cactgcatat ccttgtaaaa gaagagaaac tggctctgga acttctcgag
    26341 cagattaaga acggtgccga tttcggcaag ctggcgaaga aacactccat ttgcccgtca
    26401 ggtaaacgcg gtggtgattt aggtgaattc cgccagggcc agatggttcc ggcgttcgat
    26461 aaagtggttt tctcttgtcc ggtactggag ccaaccggtc cgctgcatac ccagttcggg
    26521 tatcacatca ttaaggtgct ttaccgtaac taacagcaag gccttctccg ggagaaggcc
    26581 ttgaactctg aatcgcactt aacctgcaac agcaatacgt ttcatatccg tcatatagcc
    26641 gcgcagtttc ttacctacct gctcaatcgc atgactgcga atcgcttcgt tcacatcacg
    26701 caactgcgcg ttatctaccg caccttccgg aatagcttta cccaggtcgc ccggttgcag
    26761 ctctgccata aacggtttca gcagcggcac acaagcgtaa gagaacagat agttaccgta
    26821 ttcagcggta tcagaaataa ccacgttcat ttcatacaga cgcttacggg cgatggtgtt
    26881 ggcaatcagc ggcagttcgt gcagtgattc atagtaagca gactcttcga tgatgccgga
    26941 atcaaccatg gtttcaaacg ccagctcaac gcccgctttt accatcgcga tcatcagtac
    27001 gcctttatcg aagtactcct gctcgccgat tttgccttca tactgcggcg cggtttcaaa
    27061 cgcggttttg ccggtttctt cacgccaggt cagcagtttc ttatcatcgt tggcccagtc
    27121 cgccatcatg ccggaggaga attcgccgga gatgatatcg tccatatgtt tctggaacag
    27181 cggtgccatg atctctttca gctgttcaga cagcgcgtaa gcacgcagtt tcgccgggtt
    27241 agagagacgg tccatcatca gggtgatgcc gccctgtttc agcgcttcgg tgatggtttc
    27301 ccaaccgaac tgaatcagtt tttctgcgta tgccggatcg gtgccttctt ccaccagctt
    27361 gtcgaagcac agcagcgagc cagcctgcaa cataccgcac agaatggttt gctcgcccat
    27421 caggtcagat ttcacttccg caacgaagga cgattccagc acgcccgcac ggtgaccacc
    27481 ggttgctgcc gcccatgctt tagcgatcgc cataccttcg cctttcggat cgttttcagg
    27541 gtgaacggca atcagcgtcg gtacgccgaa tccacgtttg tactcttcac gtacttcggt
    27601 gcctgggcat ttcggcgcaa ccatgactac ggtgatgtct ttacggatct gctcgcccac
    27661 ttcgacgatg ttgaaaccgt gcgagtaacc cagcgccgcg ccgtctttca tcagtggctg
    27721 tacggtgcgc actacatcag agtgctgctt gtccggcgtc aggttaacca ccagatccgc
    27781 ctgcgggatc agttcttcgt aagtaccaac tttaaaaccg ttttcggtcg ctttacgcca
    27841 ggatgcgcgc ttttcggcaa tcgcttcttt acgcagagcg taggagatat cgagaccaga
    27901 atcacgcatg ttcaggccct ggttcagacc ctgtgcgcca cagccgacga tgactacttt
    27961 tttaccctga aggtagctcg cgccatcggc gaattcatcg cgccccataa agcgacattt
    28021 gcccagctgt gccagctgct ggcgcagatt cagtgtattg aagtagttag ccatggtgat
    28081 tcctcgtgat gttgtgcttc ttattgttcg gttcgcgttt gcgagattga attcactata
    28141 tgacaggaaa tttattgcgg aaattgatat attcacaaca tcacattgca atttctgcaa
    28201 cgccaacatt gagggctgcg ccattggatt tacgcgatct gaaaaccttc ctgcatctgg
    28261 cggaaagccg ccattttggc cgtagcgcgc gggcaatgca cgttagcccg tctacgcttt
    28321 cgcgacaaat acagcgcctg gaagaagatc tcggtcagcc gctgtttgtg cgtgataacc
    28381 gcacggtaac gctgacggaa gcgggcgaag aactgcgcgt tttcgcccag caaacgctgt
    28441 tgcaatatca gcaattgcgt cacaccatcg atcagcaagg gccgtcgctc tctggcgaat
    28501 tacatatctt ctgctcggtg accgctgcct acagccatct gccgccgatt ctggatcgct
    28561 tccgcgcgga acacccgtca gtggagatca aactcactac cggtgatgcg gccgatgcga
    28621 tggaaaaggt ggtcactggc gaagcggatc tggcgattgc ggggaaaccg gaaaccttac
    28681 ccggcgcagt ggcgttttcg atgctggaga atctggcggt ggtgctgatt gctcccgcgc
    28741 tgccctgccc ggtgcgtaat caggtatctg tcgaaaagcc cgactggtct acagtgccgt
    28801 ttatcatggc cgatcaggga ccggtacgcc gccgcattga actgtggttt cggcgtaaca
    28861 aaatcagcaa cccaatgatt tacgccacgg ttggcgggca tgaagcgatg gtatcgatgg
    28921 tggcgctcgg ctgtggcgtg gcgctgttgc cggaagtggt gctggataac agcccggaac
    28981 cggtgcgtaa ccgcgtgatg attttagagc gcagtgatga aaaaacgccg tttgagctag
    29041 gcgtgtgtgc gcaaaaaaag cggctgcatg agccgctgat tgaggcattc tggaagattt
    29101 tgccgaacca ctaacccgcc aaaaagaacc tgaacgccgg gttattggtt tcgtcgtggc
    29161 aatcgtagcc cagttcattc agccgggttt caaaatccgg ttcatgatcg ccaagctcga
    29221 acgccgccag tacgcgcccg tagtcggtac cgtggctgcg ataatggaac agcgagatgt
    29281 tccagtgcgt acccagcgtg ttgaggaagc gcagcagcgc gcccggtgat tccgggaatt
    29341 cgaagctgta gaggcgttcc tgcaacggat gcgatggacg cccgccaacc atatagcgca
    29401 catgtagctt cgccatttcg tcgtcggaga gatcaaccac gctgtagcca ccgtcgttga
    29461 gcatctgcaa aatttcttta cgctcttcga gaccgcggct caggcgcacg ccgacaaaaa
    29521 tgcaggcgtt tttggcatca gcaaagcggt agttgaactc ggtgaccgag cgcccaccaa
    29581 gtaactggca gaatttgagg aaactgccct tttcttccgg aatggtcacc gccagtaacg
    29641 cttcacgctg ttcgcccagt tcgcagcgtt ccgagacgta gcgcagaccg tgaaagttca
    29701 cgttggcacc ggaaagaata tgcgccagcc gttcaccgcg aatgttgtgc tgggcgatgt
    29761 attttttcat ccccgccagc gccagcgcgc cggaaggttc cgccaccgcg cgcacatctt
    29821 cgaataaatc cttcatcgcc gcacagatcg catcactatc gacggtgatg atgtcgtcga
    29881 gatactcctg gcataaacgg aaggtttcgt cgccgatgcg ttttaccgcg acaccttcgg
    29941 cgaacagtcc cacgcggggc agatcaacgg gatgacccgc atccagcgcc gctttcaggc
    30001 aggcggaatc ttccgcttcc acggcgatta ctttgatttg cggcatcagt tgtttgatca
    30061 gcaccgccac acccgctgcc agaccgccgc cgccgaccgg tacaaatacg cggtcgagat
    30121 gggcgtcctg ctggagcagt tccagcgcca gcgtgccttg cccggcgatc accatcggat
    30181 ggtcgaacgg cggtacccag gtgaaaccct gctgctgtga cagttcgatc gctttcgctt
    30241 tcgcttcatc gaaatttgcg ccgtgaagca gcacttcgcc gccaaagccg cgcaccgcat
    30301 caactttgat atcggcggtg gcggttggca tgacgatcag cgccttcacg cctaaccgtg
    30361 cggaagaaaa cgcgacaccc tgcgcgtggt tacctgcaga agcggtaatc acgccgtgcg
    30421 ctttttgttc ttccgtcagg cccgccatca tggcgtatgc gccgcgcaac ttaaagctat
    30481 gaactggctg gcgatcttcg cgcttcacca gaatcacgtt atcgagacgc gacgacagtt
    30541 tttccatttt ctgtagcggc gtgacctgcg ccgcttcgta gaccggcgcg cgcagcaccg
    30601 ctcttaaata ttcggcacct tccggggtac cggacagggg ttgcgagtca gccattatta
    30661 accccccagt ttcgatttat cgcgcaccgc acctttgtcg gcgctggttg ccaggctggc
    30721 gtaggcacgc agcgcaaagg agacctgacg ttcgcgattt ttcggcgtcc aggctttgtc
    30781 gccacgagct tcctgcgctt cacgacgcgc caccagttcg gcatcgctta cctgtaactg
    30841 aatgccacgg ttcggaatgt cgatggcaat catgtcgccg tcttcaatca gaccaatgct
    30901 gccgccgctt gccgcttccg gcgagacgtg gccgatggaa agaccagagg tgccgccgga
    30961 gaagcgtccg tcggtgatca gcgcgcaggc tttaccgagc cccattgatt tcaggaagct
    31021 ggttgggtag agcatttcct gcatgccggg gccgcctttc gggccttcgt aacggatcac
    31081 caccacatcg ccagcgacaa ctttgccgcc gagaatcgct tctaccgcgt cgtcctggct
    31141 ttcgtacact ttcgccggac cggtgaattt gaggatgctg tcatcgacgc ccgcggtttt
    31201 aacgatgcag ccgttttctg cgaaattacc gtacagcacc gcaaggccgc cgtctttgct
    31261 gtaggcgtgt tccagcgaac ggatgcagcc atttgcgcga tcgtcatcga gagaatccca
    31321 acggcaatcc tgcgagaatg cctgggtggt acgaatgccc gccgggccag cgcggaacat
    31381 attttttacc gcgtcatcct gggtcagcat aacgtcgtat tgttccagcg tttgcggcaa
    31441 cgtcaggcca agtacatttt tcacatcacg gttcagtaat cccgcacgat ccagctcgcc
    31501 gagaataccg ataacaccac cagcccggtg aacatcttcc atatggtatt tttgggtgct
    31561 cggcgcaact ttacacagct gcggaacctt gcgggaaagc ttatcgatat cactcatggt
    31621 gaagtcgatt tccgcttcct gcgccgccgc cagcaggtga agtacggtgt tggtagagcc
    31681 gcccattgcg atatccaacg tcatggcgtt ttcaaacgcc gctttgctag caatattgcg
    31741 cggcagtgca ctttcgtcat tttgctcgta gtaacgtttg gtcaattcaa caatgcgttt
    31801 accggcattg aggaacagct gcttacgatc ggcgtgagtt gccagcagcg agccgttgcc
    31861 cggctgcgac aggcccagcg cttcggtcag gcagttcatt gagttagcgg taaacatccc
    31921 ggagcaggag ccgcaggtcg gacacgcgga acgttcaacc tgatcgctct gggagtcaga
    31981 aactttcggg tctgcgccct ggatcatcgc atcaaccaga tcgagcttga tgatctgatc
    32041 ggacagtttg gttttcccgg cctccatcgg gccgccggaa acaaagatca ccggaatatt
    32101 caggcgcagg gaagccatca gcatccccgg ggtgattttg tcgcagttag agatgcagac
    32161 catggcgtcg gcgcagtggg cgttgaccat atactcaacg gaatcagcga tcagttcgcg
    32221 agatggcagt gaataaagca tccccccgtg gcccatggca atcccatcat ccaccgcaat
    32281 ggtgttgaac tctttggcaa cgccgccagc cgcttcaatt tgttcggcga ccagtttacc
    32341 aagatcgcgc agatggacgt gacccggtac aaattgggtg aacgagttca caaccgcgat
    32401 aatcggctta ccgaaatcgg cgtcggtcat tccggtggcg cgccacagcg cacgagcacc
    32461 cgccatatta cgaccatgag tggtggtggc ggaacggtac ttaggcatac tttatttact
    32521 cccagtgtct gtctcgtaaa tgggacggtg cgtgccgtcc cattttttgt atttattgat
    32581 taacttgatc taaccagccc catttatctt cggtttcgcc agtgaagagg ccgaagaagg
    32641 cttgctgaat gcgtttggta accgggccgc aacggccttc accaacctga ataccgtcta
    32701 cactgcgcac tggcgtgatt tctgccgcag taccggacat aaacacttca tccgccaggt
    32761 acagtgattc gcgcgacagc acctgctcac gcacttcaat tcccagctct ttcgccagtt
    32821 tgatgatggc gtcacgggta atacccggca gcgcggagga ggtgaacggt ggggtgaaca
    32881 gcacaccatc tttcacttca aacaggtttt cgcctgcgcc ttcagagata taaccgttca
    32941 catccagcgc gataccttcc tgataaccgt ggcggcgcgc ttcgctaccc accagcaggg
    33001 aagagaggta gttaccaccg gcttttgccg ccgtcgggat ggtgtttggt gctgcgcggt
    33061 tccaggagga aaccatcgca tcgatcccct gctccagcgc ttctgcaccc agataggccc
    33121 cccacgggaa agcggcaata atcacatcgg ttgagtatcc cgctggcggg tttacgccca
    33181 tgccaacatc accaacgaag atcagcggac ggatataggc gctggtgaga ttgtttttgc
    33241 ggatcacgtc acgacaagct tccatcagct catcaatgct ctgcgaaacc gggaagcgat
    33301 agattttggc ggagtcatgc agacgctgca tatgctcacg atggcggaat acaaccggtc
    33361 ctttgtgcga gtcgtagcaa cggatgcctt caaaaaccga ggtgccatag tgcagcgcgt
    33421 gcgacatcac atgcaccttc gcgtcttccc agcgaaccat ctccccattg aaccaaatgt
    33481 aatcagcttt cttcgtggtc atttttatat tccttttgcg ctcaggcgcg gatttgttgt
    33541 gatgtggttg tgctctggca gatggcaacg tgtgcgacgt ccaccagttt atttaactga
    33601 ctaaacagta agtcgaccga ccgtgggctg gcaacggtca attcgatatt tatattttgt
    33661 gcatcgctgg cggcggccat attcattgaa cagacgtgga aaccacgatg acgcaccacg
    33721 cgtaaaacac gttctaaggt ttccggattg aagcgagccg atacattgac ctgatgttgc
    33781 atcatgataa tttctccaac atttctgaat tactggcacc aggcggcacc agcggccaga
    33841 cgttctcaag ttcgtcgatt gagacatgaa gcaggtatgg cccatcactg ttcagcatgg
    33901 tgtcgagtgc cgcttcaacc tggtctttac gggtgatgtg ttggccaggg atgccgaagg
    33961 cgctggctaa catgaggaaa tcggggttat cagtaagggt tgtttcgctg tatcgttcct
    34021 ggaaaaacag ttgctgccat tgtcgaacca tccctaaccg ttggttatcg agtaagacga
    34081 ttttcaacgg taactgcttg cgttttacgg tgcccagctc ttgcacattc atcatgaaag
    34141 agccgtcacc ggagatacag acgacggtat cgttcggtcg cgcgacttgt gcgccaaccg
    34201 ccgcaggtaa accaaaaccc atggtgccta agccgctgga ggtgatgaaa ttttccgggc
    34261 gagtgtgggc gatgtgctgg gcagcccaca tctggtgctg ccccacatct gtggtcacga
    34321 cgcaatccgc aggttttcga tccgacagtt gttttaacaa caacggcgcg tagatagcgt
    34381 caccgggatg gtcgtaacgc caggtatgtt catcacgcag ctgcgcgcag tattgctgcc
    34441 agtcattgat atttaacggc tgctgtaatg ctggtaaaag agcatttaaa tcaccctgta
    34501 aagccacatg tacctgacgc agcttgttca tttctgccgg atcgatatcc atatggataa
    34561 cgctggcgtg tggtgcgaag gtgttcagtt tgccagtcac ccggtcatca aaacgtgcac
    34621 ccacggcgat cagcagatcg cactcctgca ctgcgaagtt cgccgctttg gtgccgtgca
    34681 ttcccagcat gcccagatag tacggataat ccgcttcaac tgcgcccagc cctttcagcg
    34741 tgcaggtgac aggcattttt gtggtagcga gaaattcacg caaagccgga actgcctgcg
    34801 ccatgccaac gccaccgcca acgtacagca tcggtttttg cgcttttgcc agcatctggc
    34861 gcgcttgctc gacttcggca tgggggaaag tcacttcgtt ttcaacggtg gtgaaccacg
    34921 gttccagatc gccgctggct aactggatat cttttgggat atcgaccaga accggaccag
    34981 gacgacctga gctggcaacg tcgaatgctt cagccatgat gcgcggcaac tcttccagcg
    35041 actgcaccag aaagctgtgc ttggtacagg ctaatgacaa tcccagtaca tccacttcct
    35101 gaaatgcgtc agtgccgata aacggtgcag acacctgacc ggtgatggca acgacaggga
    35161 tggaatctaa cagtgcgtcc gcaagcccgg ttatcaggtt ggttgcacct ggaccagacg
    35221 tggcgataca tacgccagtt ttgccggtag cacgggcata accgatagcc gccattgctg
    35281 caccttgctc gtgtcggcac agcaagtgtt ccacgccacc gtcatacaat gcatcgtaaa
    35341 ccggcataat tgcgccaccc ggataaccga aaacggtgtt cacaccctgt gcccgcaacg
    35401 catgtaccac ccactgtgcg ccattcatag ttagttcccc gtcctgaatc ttgagaaaca
    35461 gaattttgtg ctgttattca ttgtctgctc ctcagttatg tttttaaggt caaaaaaaac
    35521 ccccggacct ttcggtgcgg gggtcttagt tcgttaaggc ttgatctcta agcctttcct
    35581 cgtccaagtg cagccccgca cggtgggata ataatcacca ccacgctaat cacgaccagg
    35641 ctaatcactc gtagaagggc tgtcattttg tcttttcttg catcttgttc gaaggaatgc
    35701 ctaaagagtt accatagatt ttgcgaatag cacaagata
//
LOCUS       DACBVI010000032        35736 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.032, whole genome shotgun sequence.
ACCESSION   DACBVI010000032 DACBVI010000000
VERSION     DACBVI010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.032"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(145..357)
                     /gene="rpmE"
                     /locus_tag="IU325_07790"
     CDS             complement(145..357)
                     /gene="rpmE"
                     /locus_tag="IU325_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HAP5808932.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            560..2758
                     /gene="priA"
                     /locus_tag="IU325_07795"
     CDS             560..2758
                     /gene="priA"
                     /locus_tag="IU325_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP5808933.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSNVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVAMLEF
                     NDAALQALRKKGLCDLASETPEFSDWRKNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTHRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2914..3939
                     /gene="cytR"
                     /locus_tag="IU325_07800"
     CDS             2914..3939
                     /gene="cytR"
                     /locus_tag="IU325_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HAP5808934.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSRAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4031..4990
                     /gene="ftsN"
                     /locus_tag="IU325_07805"
     CDS             4031..4990
                     /gene="ftsN"
                     /locus_tag="IU325_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HAP5808935.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5083..5613
                     /gene="hslV"
                     /locus_tag="IU325_07810"
     CDS             5083..5613
                     /gene="hslV"
                     /locus_tag="IU325_07810"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HAP5808936.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5623..6954
                     /gene="hslU"
                     /locus_tag="IU325_07815"
     CDS             5623..6954
                     /gene="hslU"
                     /locus_tag="IU325_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HAP5808937.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTITIDADYVSKHLDALVADEDLSRFIL"
     gene            7021..7947
                     /gene="menA"
                     /locus_tag="IU325_07820"
     CDS             7021..7947
                     /gene="menA"
                     /locus_tag="IU325_07820"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP5808938.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8040..8525
                     /gene="rraA"
                     /locus_tag="IU325_07825"
     CDS             8040..8525
                     /gene="rraA"
                     /locus_tag="IU325_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HAP5808939.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8610..8855)
                     /gene="zapB"
                     /locus_tag="IU325_07830"
     CDS             complement(8610..8855)
                     /gene="zapB"
                     /locus_tag="IU325_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HAP5808940.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9280..10125
                     /gene="glpF"
                     /locus_tag="IU325_07835"
     CDS             9280..10125
                     /gene="glpF"
                     /locus_tag="IU325_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HAP5808941.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10148..11656
                     /gene="glpK"
                     /locus_tag="IU325_07840"
     CDS             10148..11656
                     /gene="glpK"
                     /locus_tag="IU325_07840"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP5808942.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            11791..12801
                     /gene="glpX"
                     /locus_tag="IU325_07845"
     CDS             11791..12801
                     /gene="glpX"
                     /locus_tag="IU325_07845"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAP5808943.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            12898..13644
                     /locus_tag="IU325_07850"
     CDS             12898..13644
                     /locus_tag="IU325_07850"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HAP5808944.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAVGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13649..14077)
                     /gene="uspD"
                     /locus_tag="IU325_07855"
     CDS             complement(13649..14077)
                     /gene="uspD"
                     /locus_tag="IU325_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HAP5808945.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(14104..14403)
                     /locus_tag="IU325_07860"
     CDS             complement(14104..14403)
                     /locus_tag="IU325_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HAP5808946.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14615..15055)
                     /locus_tag="IU325_07865"
     CDS             complement(14615..15055)
                     /locus_tag="IU325_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAP5808947.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTEDVKYKADNKSSN"
     gene            15156..15755
                     /locus_tag="IU325_07870"
     CDS             15156..15755
                     /locus_tag="IU325_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1454 family protein"
                     /protein_id="HAP5808948.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            15863..16630
                     /gene="tpiA"
                     /locus_tag="IU325_07875"
     CDS             15863..16630
                     /gene="tpiA"
                     /locus_tag="IU325_07875"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP5808949.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16685..17440)
                     /locus_tag="IU325_07880"
     CDS             complement(16685..17440)
                     /locus_tag="IU325_07880"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HAP5808950.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRHDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17547..18536)
                     /locus_tag="IU325_07885"
     CDS             complement(17547..18536)
                     /locus_tag="IU325_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP5808951.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(18856..19818)
                     /gene="pfkA"
                     /locus_tag="IU325_07890"
     CDS             complement(18856..19818)
                     /gene="pfkA"
                     /locus_tag="IU325_07890"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP5808952.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(19999..20901)
                     /gene="fieF"
                     /locus_tag="IU325_07895"
     CDS             complement(19999..20901)
                     /gene="fieF"
                     /locus_tag="IU325_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HAP5808953.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21050..21550)
                     /gene="cpxP"
                     /locus_tag="IU325_07900"
     CDS             complement(21050..21550)
                     /gene="cpxP"
                     /locus_tag="IU325_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HAP5808954.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSVDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21700..22398
                     /gene="cpxR"
                     /locus_tag="IU325_07905"
     CDS             21700..22398
                     /gene="cpxR"
                     /locus_tag="IU325_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HAP5808955.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22395..23768
                     /gene="cpxA"
                     /locus_tag="IU325_07910"
     CDS             22395..23768
                     /gene="cpxA"
                     /locus_tag="IU325_07910"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HAP5808956.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24024..24698)
                     /locus_tag="IU325_07915"
     CDS             complement(24024..24698)
                     /locus_tag="IU325_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HAP5808957.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGLVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDEQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24847..25830)
                     /gene="kdgT"
                     /locus_tag="IU325_07920"
     CDS             complement(24847..25830)
                     /gene="kdgT"
                     /locus_tag="IU325_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HAP5808958.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPEAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26090..26710)
                     /gene="sodA"
                     /locus_tag="IU325_07925"
     CDS             complement(26090..26710)
                     /gene="sodA"
                     /locus_tag="IU325_07925"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HAP5808959.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            26995..28029
                     /gene="rhaT"
                     /locus_tag="IU325_07930"
     CDS             26995..28029
                     /gene="rhaT"
                     /locus_tag="IU325_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HAP5808960.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGID
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGMGMAN"
     gene            complement(28026..28964)
                     /gene="rhaR"
                     /locus_tag="IU325_07935"
     CDS             complement(28026..28964)
                     /gene="rhaR"
                     /locus_tag="IU325_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HAP5808961.1"
                     /translation="MAFCNNANLLTVFVRHIANNQLRSLAEVATVAHQIKLLKDDFFA
                     SDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADD
                     KHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVI
                     GQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAAS
                     LKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQIRVCHAQYLLQHSRLLISD
                     ISTECGFEDSNYFSVVFTRETGMTPSHWRHLNSQKD"
     gene            complement(28948..29784)
                     /locus_tag="IU325_07940"
     CDS             complement(28948..29784)
                     /locus_tag="IU325_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HAP5808962.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30072..31541
                     /gene="rhaB"
                     /locus_tag="IU325_07945"
     CDS             30072..31541
                     /gene="rhaB"
                     /locus_tag="IU325_07945"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP5808963.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVGSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRNIYQRSGIQFLPFNTLYQLHALTEQQPELIPHIAHALLM
                     PDYFSYRLTGEMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFTINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNALLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            31538..32797
                     /gene="rhaA"
                     /locus_tag="IU325_07950"
     CDS             31538..32797
                     /gene="rhaA"
                     /locus_tag="IU325_07950"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP5808964.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLKSVRAYEKEILSRRG"
     gene            32880..33704
                     /gene="rhaD"
                     /locus_tag="IU325_07955"
     CDS             32880..33704
                     /gene="rhaD"
                     /locus_tag="IU325_07955"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP5808965.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTHEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            33714..34028
                     /gene="rhaM"
                     /locus_tag="IU325_07960"
     CDS             33714..34028
                     /gene="rhaM"
                     /locus_tag="IU325_07960"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP5808966.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            complement(34069..35463)
                     /locus_tag="IU325_07965"
     CDS             complement(34069..35463)
                     /locus_tag="IU325_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HAP5808967.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQATAESAKKNSTSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKLDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKQNDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNLYFELNTLLEDGTSLYNDGNYHGRD
                     VEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPENGVVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
BASE COUNT         8810 a         9264 c         9171 g         8491 t
ORIGIN      
        1 aaataaataa gaaccagaag cctagcgcca gtcaacaaca ggaaatgata atgagttaac
       61 accccggaat ttttcagaat ttataaaagg caaattacag gcacaaaaaa agcgccgtgc
      121 ggcgcttttt tcggaaatcc ggtcttattt gctgcccggg atgttgaaac gcttgttgaa
      181 gcggtcaaca cggccaccgg tagcaacatc acgctgtttg ccagtgaaga acgggtggca
      241 cttgctgcac acgtcgaggt tcaggtcatg accaacggtg gagcggattt tcattacgtt
      301 accgcaagag cagctagcag taatttcttc gtatttcggg tgaatatctt ttttcatggg
      361 aaaacctcgg tttaaggccg cgtcgctctt ccagccctaa cgccagacac cacgcgatgt
      421 taaaagtata gcttcaatac gatcatttcg tacgaaggcg cgaaatcata cagaaattaa
      481 ccagcgtatg caaactgatc cgcactcttc tacggcaatg tgtatactaa cccaccgaat
      541 ttcaagtcag gatgatgcta tgcccgttgc ccacgttgcc ttgcccgttc cgcttccccg
      601 tacctttgac tatctgctgc cagaaggcat gacggtcaaa gccgggtgtc gcgtgcgcgt
      661 gccgtttggc aaacagcagg agcgcatcgg ggttgtggtg tcagttagca atgtcagcga
      721 gctgccgctt aacgagctga aagcggtagt tgaagtgctg gacgttgagc cggtatttac
      781 tcactccgtc tggcgattac tgctctgggc ggcagattac tatcatcatc cgattggcga
      841 tgtgctgttt catgccttgc cgattttact acgccagggg cggcctgcgg cgaacgcgcc
      901 gatgtggtac tggtttgcca ctgaacaagg tcaggcggtg gatctgaaca gcctgaaacg
      961 ctcacccaag caacaacagg cgctggcagc gttacggcaa ggcaaaatct ggcgcgacca
     1021 ggttgccatg ctcgaattta atgatgccgc gttgcaggcg ctgcgcaaaa aaggtctgtg
     1081 tgatttagca agtgaaacac cagagtttag tgactggcga aagaattatg ccgtttctgg
     1141 tgagcggttg cgattgaata ctgaacaggc caccgccgtt ggcgcaattc atagtgcggc
     1201 ggataccttc tctgcctggc tgctggcggg cgttaccggt tccggtaaaa cggaggttta
     1261 tctcagtgta ctggaaaacg tgctcgctca gggcaaacag gcgctggtga tggtgccgga
     1321 aatcggcctg acgccgcaaa ctatcgcccg ttttcgtgaa cgttttaatg cccccgtgga
     1381 agttctgcat tccggcctga acgacagcga gcgtctttcg gcgtggttga aagcgaaaaa
     1441 tggcgaggcg gcgattgtca tcggcacccg ctccgcgctg tttacgccgt ttaaaaatct
     1501 cggcgtgatt gttattgatg aagagcacga cagttcctac aaacagcagg aaggctggcg
     1561 ctatcatgcc cgcgacctgg cggtgtatcg cgcgcatagc gagcaaatcc caattattct
     1621 tggctccgca acgcccgcac tggaaacgtt atgcaacgtg cagcagaaaa aataccgcct
     1681 gctgcgcctg acgcatcggg cggggaatgc gcgtccggca attcaacacg tgctggattt
     1741 aaaaggtcag aaggtgcagg ctggtctggc tccggcgtta atcacccgta tgcgccagca
     1801 tttacaggca aacaatcagg ttatcctctt tcttaaccgc cgtggctttg cgcctgcctt
     1861 gctgtgccac gactgcggct ggattgccga atgtccacgt tgcgatcact actacacgct
     1921 gcatcaggcg caacagcatt tgcgctgcca ccattgcgac agtcagcgtc cggtgccgcg
     1981 ccagtgccct tcctgcggtt ccacgcacct ggtacccgtg gggctgggca ccgaacagct
     2041 tgaacagaca ctcgcgccgt tgttccctgg tgtccccatt tctcgtatcg accgcgatac
     2101 caccagccgc aaaggcgcac tggaacaaca actggcggaa gtacatcgcg gtggcgcgag
     2161 gattttgatc ggcacgcaaa tgctggcgaa aggtcaccac tttccggatg tgacgctggt
     2221 ggcgttactg gacgtagacg gcgcgctgtt ttctgccgat tttcgctcgg cagagcgttt
     2281 cgcccagctt tacacccagg tcgccggtcg tgccgggcgt gcgggtaaac agggcgaagt
     2341 ggtgctgcaa acgcaccatc cggaacatcc tctcttgcaa acattgctct ataaaggcta
     2401 cgacgccttt gccgaacagg cgctggctga acgacgcatg atgcaactgc cgccgtggac
     2461 cagccatgtg attgtgcgtg cggaagatca taacaatcag cacgcgccgc tgttcctgca
     2521 acaactgcgt aatctgatcc tctccagccc gctggcagac gacaaactgt gggttctcgg
     2581 tcccgttccg gcactggcac ctaaacgtgg cggccgctgg cgctggcaaa ttttgttgca
     2641 gcacccttcc cgcgtgcgat tacagcacat catcaatggt acgctggcgc ttattaatac
     2701 aataccggat tcccgtaagg tgaaatgggt gctggatgtc gatcccatcg agggctaatg
     2761 cgctcacgat gcgaggcgga tcgaaaaatt caatattcat cacacttttc atgaaaattc
     2821 tgtaaccgtt ttcacgcgct atctgctaaa aatgttgccg atgtgaagta aacatggaag
     2881 tagtacgcct gacgtgccag gcgaggagtg agtgtgaaag cgaagaagca ggaaactgcc
     2941 gcgaccatga aagacgttgc cctcaaggca aaagtctcta cagcgactgt ctcccgagca
     3001 ttaatgaatc ccgataaagt ctcccaggcc acccgtaatc gggttgaaaa agcggcccgg
     3061 gaagtgggtt atttaccgca gcctatgggg cgcaacgtca agcgtaatga atcccgcacc
     3121 attctggtga ttgtcccgga tatctgcgat cccttcttta gcgaaattat tcgcggtatc
     3181 gaagttacgg cggcaaatca cggatatctg gtgctgattg gcgactgtgc gcatcaaaat
     3241 cagcaggaaa aaacctttat cgatttgatc atcaccaagc aaattgatgg catgttgttg
     3301 ctgggttcaa ggctgccgtt tgatgccagc attgaggaac agcgtaatct gccgccgatg
     3361 gtgatggcga acgaatttgc accggaactg gagctgccta ccgttcatat cgacaatctg
     3421 accgccgcat ttgatgcagt aaattattta tatgagcaag ggcataaacg gattggctgt
     3481 atagccggtc ccgaagagat gccgctgtgt cactaccgcc tgcaaggcta tgttcaggcg
     3541 ctgcgtcgct gcggcattat ggttgatccg caatacatcg cccgtggcga cttcaccttc
     3601 gaagccggaa gcagagcaat gcaacagctg cttgatcttc cacaaccgcc tactgctgtc
     3661 ttctgccata gcgatgtgat ggcgctcggc gcactttctc aggcaaaacg tcaggggctg
     3721 aaagtcccgg aagacctttc cataatcggt tttgataaca tcgacctgac gcaattttgt
     3781 gatccgccgc tgacaaccat cgcgcagccg cgttacgaaa tcggtcggga agctatgctg
     3841 ttattgcttg atcaaatgca ggggcaacac gttggcagtg gctctcgttt aatggactgc
     3901 gaacttatca tccggggatc aacacgcgcg ttaccttaaa gtaaaccttt aagactgccg
     3961 tgactggtca aaggcccgcc gcttaagtaa catggcgggc tgacgaacga ataaatacag
     4021 cgaaacgata gtggcacaac gagattatgt acgccgcagc caaccggcac cttcgcggcg
     4081 aaaaaagagc acctcacgga aaaagcaacg aaatctgcct gcggtttctc ccgctatggt
     4141 cgctattgct gccgccgttc ttgtgacctt tatcggtggt ctgtacttca ttacgcatca
     4201 caagaaagaa gagtccgaga cgctgcaaag ccagaaagtg accggaaacg gactaccacc
     4261 aaaaccagaa gaacgctggc gctacattaa agagctggaa agtcgccagc cgggtgtgcg
     4321 tgcgcccaca gaaccttctg ccggtggtga agtgaaaacg ccggagcaac tgacaccaga
     4381 acaacgccag cttcttgaac aaatgcaggc tgatatgcgc cagcagccaa cgcagctggt
     4441 tgaagttccg tggaatgagc agacgccgga acagcgtcag caaacgctac agcgccagcg
     4501 tcaggcgcag caattagcgg agcagcaacg cctggctcag cagtcgcgta cgactgaaca
     4561 aagctggcag cagcagacgc gtacgtcgca agccgcaccg gtacaggcgc aaccgcgcca
     4621 gtctaaaccg gcttctactc agcagccgta ccaggatctg ctgcaaactc ctgcgcacac
     4681 gaccgcgcag tcgaagccgc agcaagctgc gccggttgct cgtgccgctg acgcgccaaa
     4741 accgacggcg gagaaaaaag acgaacgccg ctggatggtg cagtgcggtt cgttcagagg
     4801 cgcggaacag gcagagacgg tacgtgctca gctggcgttt gaaggctttg actcgaagat
     4861 caccaccaac aatggctgga atcgtgtggt cattggcccg gtgaaaggca aagagaacgc
     4921 agacagcacc ctcaatcgct tgaagatggc gggtcataca aactgcattc ggctcgccgc
     4981 cgggggttga aaccctcaaa atccccccca tctataattg cattatgccc cgtacttttg
     5041 tacggggttt gtactctgta ttcgtaacca aggggtcagc tcgtgacaac tatagtaagc
     5101 gtacgccgta acggccatgt ggtcatcgct ggtgatggtc aggccacgtt gggcaatacc
     5161 gtaatgaaag gcaacgtgaa aaaggtccgc cgtctgtaca acgacaaagt catcgcgggc
     5221 tttgcgggcg gtactgcgga tgcttttacg ctgttcgaac tgtttgaacg taaactggaa
     5281 atgcatcagg gccatctggt caaagccgcc gttgagctgg cgaaagactg gcgtaccgat
     5341 cgcatgctgc gcaaacttga agcactgctg gcagtcgcgg atgaaaccgc ttcgcttatc
     5401 atcaccggta acggtgacgt ggtgcagcca gaaaacgatc ttattgctat cggctccggc
     5461 ggcccttacg cccaggctgc ggcgcgcgcg ctgttagaaa acactgaact tagcgcccgt
     5521 gaaattgctg aaaaggcgtt ggatattgca ggcgacattt gcatctatac caaccatttc
     5581 cacaccatcg aagaattaag ctacaaagcg taaggatctc ccatgtctga aatgacccca
     5641 cgcgaaatcg tcagcgaact ggataagcac atcatcggcc aggacaacgc caagcgttct
     5701 gtggcgattg ctctgcgtaa ccgctggcgt cgcatgcagc tcaacgaaga gctacgccat
     5761 gaagtgaccc cgaaaaatat cctgatgatc ggcccgaccg gtgtcggtaa aactgaaatc
     5821 gcccgtcgtc tggctaagct ggcgaatgcg ccgttcatca aagttgaagc gaccaaattc
     5881 accgaagtgg gctatgtcgg taaggaagtg gattctatta ttcgcgatct gaccgatgcc
     5941 gccgtgaaaa tggtacgcgt ccaggctatc gagaaaaacc gttatcgcgc tgaagaactg
     6001 gcagaagaac gtattctcga cgtgctgatc ccacctgcta aaaacaactg gggacagacc
     6061 gaacagcagc aggaaccgtc cgctgctcgt caggcattcc gcaagaaact gcgtgaaggc
     6121 cagcttgatg acaaagaaat cgagatcgat cttgccgcag caccgatggg cgttgaaatt
     6181 atggctcctc cgggcatgga agagatgacc agccagctgc agtccatgtt ccagaacctg
     6241 ggcggccaga agcaaaaagc gcgtaagctg aaaatcaaag acgccatgaa gctgctgatt
     6301 gaagaagaag cggcgaaact ggtgaatccg gaagagctga agcaagacgc tatcgacgct
     6361 gttgagcagc acgggatcgt gtttatcgac gaaatcgaca aaatctgtaa gcgcggcgag
     6421 tcttccggtc cggatgtttc tcgtgaaggt gttcagcgtg acctgctgcc gctggtagaa
     6481 ggttgcaccg tttccaccaa acacgggatg gtcaaaactg accacattct gtttatcgct
     6541 tctggcgcgt tccagattgc gaaaccgtct gacctgatcc cggaactgca aggtcgtctg
     6601 ccaatccgcg ttgagctgca ggcactgacc accagcgact tcgagcgtat tctgaccgag
     6661 ccgaatgcct ctattaccgt gcagtacaaa gcactgatgg cgactgaagg cgtaaatatc
     6721 gagtttaccg actccggtat taagcgcatc gcggaagcgg catggcaggt gaacgaatct
     6781 accgaaaaca tcggtgctcg tcgtttacat accgttctgg agcgtttaat ggaagagatt
     6841 tcctacgacg ccagcgattt aagcggtcaa accatcacta ttgacgcaga ttatgtgagc
     6901 aaacatctgg atgcgttggt ggcagatgaa gatctgagcc gttttatcct ataatcgcgt
     6961 tcaatcattt tcatcattgt ttgatggggc tgaaaggccc catttttatt ggcgcgtatt
     7021 atgactgaac aacaaattag ccgaactcag gcgtggctgg aaagtttacg acctaaaacc
     7081 ctccccctcg cctttgctgc aattatcgtc gggacggcgc tggcatggtg gcaaggtcac
     7141 ttcgatccgc tggtcgccct gctggcactg attaccgccg ggctattaca gatcctttct
     7201 aacctcgcca atgattacgg cgatgcggta aaaggcagcg ataaacctga ccgtatcgga
     7261 ccgctacgcg gcatgcaaaa aggggtcatt acccagcaag agatgaaacg ggcgctcatt
     7321 attaccgtcg tgctcatctg tctctccggg ctggcactgg ttgcagtggc gtgccatacg
     7381 ctggccgatt ttgtcggctt cctgattctt ggcgggttgt cgatcattgc cgctatcacc
     7441 tacaccgtgg gcaatcgtcc ttatggttat atcggtctgg gtgatatttc cgtactggtt
     7501 ttctttggct ggttgagcgt catgggcagc tggtatttac aggctcatac attgattccg
     7561 gcactgatcc ttccggcgac cgcatgcggc ctgctggcaa cggcagtact gaacattaat
     7621 aacctgcgtg atatcaatag cgaccgcgaa aatggcaaaa acacgctggt ggtgcgctta
     7681 ggtgaagtga acgcgcgtcg ttatcatgcc tgcctgctga tgggctctct ggtgtgcctg
     7741 gcgctgttta atctcttttc gctgcatagc ctgtggggct ggctgttcct gctggcggca
     7801 ccattactgg tgaagcaagc ccgttatgtg atgcgggaaa tggacccggt ggcgatgcga
     7861 ccgatgctgg aacgcactgt caagggagcg ttactgacta acctgctgtt tgttttaggg
     7921 atattcctaa gccagtgggc agcataactg acaaatatca attaacaatt gatgattttg
     7981 ccaacagcct acatagcgcg atatactgaa aattctcgca gcaactgaat gttaagccta
     8041 tgaaatacga tacttccgag ctttgtgaca tctatcaaga agatgttaac gtcgtggaac
     8101 cgctgttctc caactttggc ggacgggcgt cgtttggcgg acaaataatc acggtaaaat
     8161 gtttcgagga caacgggttg ctgtacgatc tgctcgaaca gaatggccgt ggtcgtgttc
     8221 ttgtcgtcga tggcggtggt tctgttcgtc gcgcactggt cgatgctgaa ctggcgcgtc
     8281 tggcagtaca aaatgaatgg gaaggtctgg tcatttacgg cgcggtgcgt caggtagatg
     8341 acctggaaga gttggatatc ggcatccagg cgatggcggc aattccggtt ggtgctgctg
     8401 gcgaaggtat tggcgaaagc gatgtccgcg tcaattttgg cggtgtcacc ttcttctccg
     8461 gcgaccatct ttatgccgac aataccggga ttattctttc agaagatccg ttggatattg
     8521 aatgatagaa aaggcaccgc aaggtgcctt ttttctgcgt tacctgttgg cctacacagt
     8581 aaagaaatta cgcggaagat gaagcgtaat cagacctctt ccatgcgacc cagcagggcc
     8641 tgcagacgtt cctgccagcc gttctgctgt tctttcagat ggttgttctc acgctccagc
     8701 tcttcgcgct gatgctgggc attttgaact tcctgcgaca gtgagttgtt tttttctttc
     8761 agctcttcga tttccatctg caacagagtg atggtatcaa tcgcctgctg tacttttgct
     8821 tccagtttct caaacacttc taatgacatt gtcatacctc tcctgaattg caaggcgttg
     8881 atggataaaa atcctcgtcc cgattaccgg tgacgcctta ataaatacga gcgcacttta
     8941 gttagctccg attgtatgaa gccgcgccat cgctgtccag cggcacgcct tgcagattac
     9001 ggtttgccac acttttcatc cttctcctgg tgacataatc cacatcaatc gaaaatgtta
     9061 ataaatttgt tgcgcgaatg atctaacaaa catgcatcat gtacaatcag atggaataaa
     9121 tggcgcgata acgctcattt tatgacgaag cacacacatt ttaagttcga tatttctcgt
     9181 ttttgctcgt taacgataag tttacagcat gcctacaagc atcgtggagg tccgtgactt
     9241 tcacgcatac aacaaacatt aactcttcag gatccgatta tgagtcaaac atcaaccttg
     9301 aaaggccagt gcattgctga attcctcggt accgggttgt tgattttttt cggggtgggt
     9361 tgcgttgcag cactaaaagt cgctggtgcg tcttttggtc agtgggaaat cagtgtcatt
     9421 tggggactgg gagtggcaat ggccatctac ctgaccgcag gggtttccgg cgcgcatctt
     9481 aatcccgctg ttaccattgc attgtggctg tttgcctgtt tcgacaagcg taaagttatt
     9541 ccttttatcg tttcacaagt tgccggcgct ttctgcgctg cggctttagt ttacgggctt
     9601 tactacaatt tatttttcga cttcgagcag actcatcaca ttgttcgcgg cagcgttgaa
     9661 agtgttgatc tggctggcac tttctctact taccctaatc ctcatatcaa ttttgtgcag
     9721 gctttcgcag ttgagatggt gattaccgct attctgatgg ggctgatcct ggcgttaacg
     9781 gacgatggca acggtgtacc acgcggccct ttggctccct tgctgattgg tctactgatt
     9841 gcggtcattg gcgcatctat gggcccattg acgggttttg ccatgaaccc agcgcgtgac
     9901 ttcggtccga aagtctttgc ctggctggcg ggctggggca atgtcgcctt tactggcggc
     9961 agagacattc cttacttcct ggtgccgctt tttggcccta tcgttggcgc gattgtaggt
    10021 gcatttgcct accgcaaact gattggtcgc catttgcctt gcgatatctg tgttgtggaa
    10081 gaaaaggaga ccacaactcc ttcagaacaa aaagcttcgc tgtaatatga ctacgggaca
    10141 attaaacatg actgaaaaaa aatatatcgt tgcgctcgac cagggcacca ccagctcccg
    10201 cgcggtcgta atggatcacg atgccaatat cattagcgtg tcgcagcgcg aatttgagca
    10261 aatctaccca aaaccaggct gggtagaaca cgacccaatg gaaatctggg ccacccaaag
    10321 ctccacgctg gtagaagtgc tggcgaaagc cgatatcagt tccgatcaaa ttgcagctat
    10381 cggtattacg aaccagcgtg aaaccactat tgtctgggaa aaagaaaccg gcaagcctat
    10441 ctataacgcc attgtctggc agtgccgtcg taccgcagaa atctgcgagc atttaaaacg
    10501 tgacggttta gaagattata tccgcagcaa taccggtctg gtgattgacc cgtacttctc
    10561 tggtaccaaa gtgaagtgga tcctcgacca tgtggaaggc tctcgcgaac gtgcgcgtcg
    10621 tggtgaattg ctgtttggta cggttgatac gtggcttatc tggaaaatga ctcagggccg
    10681 tgtccatgtg accgattaca ccaacgcctc tcgtaccatg ttgttcaaca tccataccct
    10741 ggactgggac gacaaaatgc tggaagtgct ggatattccg cgcgagatgc tgccagaagt
    10801 gcgtcgttct tccgaagtgt acggtcagac taacattggc ggcaaaggcg gcacgcgtat
    10861 tccaatctcc gggatcgccg gtgaccagca ggccgcgctg tttggtcagt tgtgcgtgaa
    10921 agaagggatg gcgaaaaata cctacggcac tggctgcttt atgctgatga acaccggcga
    10981 gaaagcggtg aaatcagaaa acggcctgct gaccaccatc gcctgtggcc caactggcga
    11041 agtaaactat gcgctggaag gtgcggtgtt tatggcgggc gcatccattc agtggctgcg
    11101 cgatgagatg aagttgatta acgacgcgta cgattccgaa tatttcgcca cgaaagtgca
    11161 aaacaccaat ggtgtatatg tggttccggc atttaccggg ctgggtgcgc catactggga
    11221 tccgtatgcg cgcggggcga ttttcggtct gactcgtgga gtgaacgcta accacattat
    11281 ccgcgcgacg ctggagtcta ttgcttatca aacgcgtgac gtgctggaag cgatgcaggc
    11341 cgattctggt attcgtctgc acgccctgcg cgtggatggc ggcgcagtgg cgaacaattt
    11401 cctgatgcag ttccagtccg atattctcgg tacgcgcgtt gagcgcccgg aagtgcgcga
    11461 agtcaccgca ttgggtgcgg cctatcttgc tggtctggcg gttggcttct ggcagaacct
    11521 cgacgagctg caagagaaag cggtgattga acgcgagttc cgtccaggca tcgaaaccac
    11581 tgagcgtaat taccgttacg caggctggaa aaaagcggtg aaacgcgcga tggcgtggga
    11641 agaacacgac gagtaatgta aatgccggat gaagcgttta tgccgcatcc ggtagtcctg
    11701 aatcgtgcgg gggcaacccc gcacacatca ataatcccgc ccttcccctg tgctacactt
    11761 cgcgccattc cttactgctt agagtttgct atgagacgag aacttgccat cgaattttcc
    11821 cgcgtcaccg aatcagcggc gctggctggc tacaaatggt taggacgcgg cgataaaaac
    11881 accgcggacg gcgcggcggt aaacgccatg cgtattatgc tcaaccaggt caacattgac
    11941 ggcaccatcg ttattggtga aggtgaaatc gacgaagcac cgatgctcta cattggtgaa
    12001 aaagtcggta ctgggcgcgg cgacgcggta gatattgctg ttgatccgat tgaaggcacg
    12061 cgcatgacgg cgatgggcca ggctaacgcg ctggcggtgc tggcagtagg cgataaaggc
    12121 tgtttcctca acgcgccaga tatgtatatg gagaagctga tcgtcggacc gggagccaaa
    12181 ggcaccattg atctgaacct gccgctggcg gataatctgc gcaatgtagc ggcggcgctc
    12241 ggcaaaccgt tgagcgaact gacggtaacg attctggcta aaccacgcca cgatgccgtt
    12301 atcgctgaaa tgcagcaact cggcgtacgc gtatttgcta ttccggatgg cgacgttgcg
    12361 gcctcaattc tcacctgtat gccagacagc gaagttgacg tgctgtacgg tattggtggc
    12421 gcgccggaag gcgtggtttc tgcggcggtg atccgcgcat tagatggtga catgaacggt
    12481 cgtctgctgg cgcgtcatga cgtcaaaggc gacaacgaag agaatcgtcg cattggcgag
    12541 caggagctgg cacgctgcaa agcgatgggc atcgaagccg gtaaagtatt gcgtctgggc
    12601 gatatggcgc gcagcgataa cgtcatcttc tctgccaccg gtattaccaa aggcgatctg
    12661 ctggaaggca ttagccgcaa aggcaatatc gcgactaccg aaacgctgct gatccgcggc
    12721 aagtcacgca ctattcgccg cattcagtcc atccactatc tggatcgcaa agacccagaa
    12781 atgcaggtgc acatcctctg attgatttga tcgattgagc cttccagtcc ttcgggactg
    12841 gaattttttt gttcggagaa cgaagataag gcaagtcaat caaaacagga gaaaaacatg
    12901 gctgattggg taacaggcaa agtcactaaa gtgcagaact ggaccgacgc cctgtttagt
    12961 ctcaccgttc acgcccccgt gcttccgttt accgccgggc aatttaccaa gcttggcctt
    13021 gaaatcgacg gtgaacgcgt ccagcgcgcc tactcctatg ttaactcgcc cgataatccc
    13081 gatctggagt tttacctggt caccgtcccc gatggcaaat taagcccacg attggcggca
    13141 ctgaaaccag gcgatgaagt gcaggtggtt agcgaagcag ctggcttctt tgttctggat
    13201 gaagtaccag attgcgaaac gctatggatg ctggcaaccg gtacagcgat tggcccttat
    13261 ttatcgattc tgcaactggg caaagattta gatcgcttca aaaatctggt cctggttcac
    13321 gccgcacgtt atgccgccga cttaagctat ttgccactga tgcaagaact ggaaaaacgc
    13381 tacgaaggca aactgcgcat tcagacggtg gtcagtcggg aaacggcagc ggggtcgctc
    13441 accggacgga taccagcgtt aattgaaagt ggtgaactgg aaagcgcggt tggcctgccg
    13501 atgaataaag aaaccagcca tgtgatgctg tgcggcaatc cacagatggt gcgcgatacg
    13561 caacagttgc tgaaagagac ccggcagatg acgaaacatt tacgtcgccg accgggccat
    13621 atgacagcgg agcattactg gtaagcggtt acttatcgat aaacggcacg atgagcaaat
    13681 ccgcactcaa cttattgatc atcccgcgat atgccggcat caaacggttg ataaatgagt
    13741 ggtgatgacc acagacaagg aggtcgcact gctctttttg cataatttcc agcagtgttt
    13801 ccggcatttc tccgcgttca atacgcagtt ttgtcttcgg ccattgaata tttttcgtca
    13861 gtttatacag cttgttatcc gacttattct tcaacaattg aagaatatct tctgttgcag
    13921 ggaagtagat acccggatac aactcgctta agccatcatc aatatgaatt aacgtcaggt
    13981 gagcgtcatt atgtctggcg agctccaggg ctttattcac cagtaaggca tcttcttcat
    14041 tcccggaaat tgccacgcca atgtgtttat aagccatgtt taactccttc taaagcaact
    14101 ccatcaagct aacgaacgca gtgataactc aaaaataatc atctcggcct ggcatgagaa
    14161 ggtgaaggcc gcatcaagct caacttgccc ctccagatcc ttaatcttat aggccacgct
    14221 ggccccttca tctgcagagg atgcagcggc ggcaagctct ttcagtttgg tcataaagga
    14281 ctctgcttct tcgcgggtgg caaaaaagcg cgaaaactta ctggtacagt tgtcgttatc
    14341 aatcaccgta ccgatatcaa tcgcacatcc tttagtactg catttatcta cgacatcttt
    14401 catagggcac ctctgtatat tcgcctctct gtcgattacg acttaagaat aatgcgccgt
    14461 taagggaaga acaaacaagt aatgatctta tggattacca ttttttttga tattgcttaa
    14521 ggtgaagagt tcagaaggat tttccgggta ttgtcatttg catcgtggaa ataacaatcc
    14581 cggaaaaaaa tagcagtaag agatatatta aaggttaatt acttgattta ttgtcggctt
    14641 tatacttcac atcctcggta tctttaccat atttattttc gccttgtgtg ccaacaaacg
    14701 caccaagatc gataagcatc atcaccagaa tcaacgtcgg gacaaaacgc cccaccgccc
    14761 attgccagac acccggtaaa atcgcccagt tacccgccag cagcatccac gccacaatca
    14821 tcagaaatgc ccatgcgccg gaacgcccgc gatcatgcaa gcgcttaacg gttactgccg
    14881 ctgttggcca gagcaagcac acaaggcaaa acgccgcagt ctgaatatcg agtaaatttt
    14941 tacccgccag tgagaaaagc accagcatgc ctgcgaacca caggcctatc caaatccaga
    15001 aatcacggcg tccaatacgc cctttaaatg agaataacca ttgctgtatg gtcatgtaag
    15061 ttccttgatg gttgtctttt ccaggattct acccttttga caagggcgac agatatcgtt
    15121 ttaatcggag ccagtcataa caaaaggtac tgtcaatgaa gccagggtgt acgctgtttt
    15181 ttctcttatg ttctgcatta accgttacaa caacggcgca tgcacaaaca ccagatacgg
    15241 caacgaccgc gccttatctg ctggctggag cccctacttt cgatctctct atcagccagt
    15301 ttagagaaga ctttaacagc cagaatccca gcctgccact gaacgaattt cgtgccatcg
    15361 acagcagtcc cgacaaagcc aatctcactc gtgctgccag taaaattaat gagaacttgt
    15421 atgcttctac agcgctggag cgcggtacct taaaaatcaa aagcattcaa atgacctggc
    15481 tacccatcca ggggccagag caaaaagccg cgaaagcgaa agctcaggaa tacatggcag
    15541 cggtgatccg cacactcacc ccattaatga ccaaaacaca aagccagaaa aaactgcagt
    15601 cgctactaac ggcggggaaa aacaaacgtt attacaccga gacagaaggt gcactgcgtt
    15661 atgttgtcgc ggacaacggc gaaaaggggc tgaccttcgc tgttgaaccg attaagctgg
    15721 cgctatctga atcgcttgaa ggtttgaata aatgacaaaa agcaaagcct ttgtgccgat
    15781 gaatctctat actgtttcac agacctgctg ccctgcgggg cggccatctt cctttattcg
    15841 cttataagcg tggagaatta aaatgcgaca tcctttagtg atgggtaact ggaaactgaa
    15901 cggcagccgc cacatggttc acgagctggt ttctaacctg cgtaaagagc tggcaggtgt
    15961 tgctggctgt gcggttgcaa tcgcaccacc ggaaatgtac atcgatatgg cgaagcgtga
    16021 agctgaaggc agccacatca tgctgggtgc gcaaaacgtg gacctgaacc tgtccggcgc
    16081 attcaccggt gaaacctctg ctgctatgct gaaagacatc ggcgcacagt acatcatcat
    16141 cggccactct gaacgtcgta cttaccacaa agagtctgac gaactgatcg cgaaaaaatt
    16201 cgcggtgctg aaagagcagg gtctgactcc ggttctgtgc atcggtgaaa ccgaagctga
    16261 aaacgaagcg ggcaaaactg aagaagtttg cgcacgtcag atcgacgcgg tactgaaaac
    16321 tcagggtgct gcagcattcg aaggtgcggt aatcgcttac gaacccgtat gggcaatcgg
    16381 tactggcaaa tctgcaactc cggcgcaggc acaggctgtt cacaaattca tccgtgacca
    16441 catcgctaaa gttgacgcta acatcgctga acaagtgatc atccagtacg gcggctctgt
    16501 aaacgcgtct aacgctgcag aactgtttgc tcagccagac atcgacggcg cgctggttgg
    16561 cggtgcttct ctgaaagctg acgctttcgc agtgatcgtt aaagctgcag aagcggctaa
    16621 acaggcttaa gtctgacagg tgccggattt catatccggc acttactttc cttaactcct
    16681 cgccttaacg caaaatctca cactgatgat cctggatttc ctcggctgaa gcacggttca
    16741 gcgtcagtag atttcgttgt gtcgccagca aaacaaacga attatcactc tgccgcacca
    16801 tcgccagccc gtagcttccc atatgttccc gcgcctcagg cacttcctct gcaagcatca
    16861 taaatgggct gcgttgtacc agttcgcttt ccgttacccg acgcgccagg tactcatgcc
    16921 cacgcaaacc acctggcagt ggcaaccagc ggctgctgat gttagccaga ttgttatcca
    16981 gctgttcgcg cacatcatga cgaatacaag agatatgaat atgaaaatgg ttttgcgtac
    17041 gcccggtgcg agagttgatc gccaaagaaa ccgcgcgatc gggaaccggc tggccgtatt
    17101 ttttgctcat aaaatcacgc gcctgccagg ccaaccaaaa gaagttcggc gttgaaggat
    17161 cggtcaacaa aggactttcc gtgccgttaa tacgatacgt tggcatcaac agatattgca
    17221 gtgggccatt aagatctttt aaaaccacgt atccggcatt gggtttgact tccgcacatg
    17281 gcgaaggatt ttgattttgc tgttgattgg gcaaacattc ctcaaggaca atcttacgta
    17341 gtgtatccga ctcttcaccg gttaatttcc agtaaccaat accggcagcc acaacggcga
    17401 taactatcat caccaaaaaa agaagacccg cttttttcat ctttttttcc ctgtacctca
    17461 aagagatgca agggtaacgc aaaatcgtga caaataaaaa acccggtcgc gagaaaccac
    17521 cgggtttgat aattatcctg gggaaatcag cgtttgctga tctgatcgaa cgtaccgccg
    17581 ttagcaaaat gctctttttg cgctttcgtc cagccgccga actcttcatc aatggtgaat
    17641 aacttcagct ttggaaacgc attttcgtac tttttcgcca cctcagcatc gcgcggacgg
    17701 tagtagtttt tcgcggcaat ttcctgacct tctggcgagt agagatattt cagataggct
    17761 tccgccacct ctttggtgcc ttttttctcg accactttat cgaccaccga cacggttggc
    17821 tctgcgagga tagactcact cggcgtaacg atttcgaatt tatctttccc cagctcattc
    17881 gctgccagca gagcttcgtt ttcccaggcg atcagtacat cgccaatccc gcgctcgaca
    17941 aaggtattag tggagccgcg cgcgccagaa tccagaactt cgacgttttt atacagtgcc
    18001 cgaacaaaat cctgtgcttt tgcctgatcg ttgttgttgt gatgcagcgc gtagccccag
    18061 gctgccaggt agttccagcg cgcgccacca gagcttttcg gattaggcgt gatcaccgaa
    18121 acacccggtt taatcagatc gttccagtca tggatctgct tcggattgcc cttacgcacc
    18181 aggaaaacaa tggtggaagt gtacggtgcg gagttatccg gcagacgttt gatccactct
    18241 ttatcaatcc gcccgcgttc cgcaatcgcg tccacgtcat aggccagagc cagagtgaca
    18301 acatcagctt caataccatt gattaccgac gtcgcttgtt tgcctgaacc accgtgtgac
    18361 tgacgaatca ccacgttatc gccagtttgc tgtttccagt gggcgctgaa tgccttgttg
    18421 tactgttcgt acaattcgcg cgttggatca tatgaaacgt taagaagctg aatatccttt
    18481 gccataacgc tggttgccgc cagcaaaaat gttaacccta cgccccactt gttcatcgcc
    18541 cgactctctt atgttgtgtt gtgatgagca aagcgtgcca gaaggttaac caaacattaa
    18601 agaataaaaa aagattggct ataacttgcg ggtatatgtt gggggattaa aaaggcggaa
    18661 gaaatccgcc tcatattgct gacaaagtgc gctttgtcca tgccggatgc ggcacaaacg
    18721 ccttatccga cctataaaag tttgcaaatt caatagatta cagaattcat gtaggcctga
    18781 taagcgaagc gcatcaggcc tttttgcttc tgtcatcggt ttcaggctaa aggaatctgc
    18841 ctttttccga aatcattaat acagtttttt cgcgcagtcc agccagtcgc ctttgaacgg
    18901 acgcttcatg ttttcgatag cgtcgatgat gtcgtggtga accagctgtt cgttctggat
    18961 acctacgcaa cgaccgccgt aacctgccag cagcagatcg atagcgtaag cgcccatacg
    19021 ggaagccaga atacggtcgt aaggcaccgg agaaccaccg cgctggatgt ggcccagcac
    19081 agttgcgcgg gtttcacgac cggtttcttt ctcgatgaaa tgcgccagtt cgtcaacatc
    19141 acacatatgt tcggtaatcg ccacgatcgc gtgtttttta cctttcgcga tacccgcttt
    19201 gatttcgttt accaggtctt cacggctgaa ttcaacttcc ggaaccacaa cgaattcaca
    19261 gccaccggca atggccgcag ccagggtcag atcgccacaa taacggccca tcacttccac
    19321 cacggaaata cgctggtgag aagaagaggt gtcacgcaga cggtcgatcg cttctacaac
    19381 ggtgctcagc gcagtgaaga aaccgatagt gtagtcagtg cctttgatgt cgttgtcgat
    19441 agtgcccggc agaccgatgc acgggaagcc catttcggtc agacgcattg cacccatgta
    19501 ggaaccgtca ccgccgataa ccaccagcgc gtcgatacca cgttttttca ggttttcgat
    19561 agccacggcg cggatgttct cgtcgcggaa ttccgggaaa cgcgcagaac cgaggaacgt
    19621 accaccacgg ttgatcatgt cagaaacgct gtaacggtct agctgtacca tacggtcttc
    19681 atacagaccc agatagccgt cataaatgcc cattacttcc agaccttctg tcagcgcaga
    19741 acgaacaacc ccgcgaattg cggcgttcat gcctggcgca tcaccgccgc ttgtcaacac
    19801 accgattttc ttaatcatga ctacctctga actttggaat gcaaaatgaa atctgttgcc
    19861 ggaagtcttc ttgcacatcg aagtgatcca acgaatgtgc aaatagtata acaatcactt
    19921 cctgctgaat tgattcaggt caggccaaat ggcggtattt tatacacaaa atgcgggtct
    19981 ggctctcttt tatactgatt atgaaagcat agaccgttta ccctccctgg gtacgacgga
    20041 acaggggtcc tgatggataa tcacatccga tcccggaaaa cgccgtaaaa tagcctgctc
    20101 tacctgatcc gccaccatat gtgcctgaac caaaggcaga gagtcttcca tttccaaatg
    20161 aatctgaata aagcgggtcg gccctgactg ccgcgtgcga agatcgtgag cgccgctaac
    20221 acccggccag gaagtcacga tatcaataat ttcttgccgt tcttcatcag gcaatgcgcg
    20281 atccagtaat gactgtaccg cctcatatcc catgcgtaac gcgctatata aaatatagat
    20341 gccgattccc aatgcaaaca gagcatcggc gcgatgccag ccgtaccagg acaaccccag
    20401 cgccagcaga attgcgccgt tcatcataac atcagactgg taatgtagca tatcagcccg
    20461 caccgcctgg ctttgcgtcc ggcgcaccac ccaccgctga aacgagacaa ggataatcgt
    20521 acaaattagc gccacaattg tcacgattac cccgacgcct ggatctgtca tcggtgttgg
    20581 agatatcaga tgttgaatac ccgtcaaaaa gaggaatagt gccgaaccgg agataaacat
    20641 actttgcgcc agcgccgcga gggactcagc tttaccgtga ccaaacgagt gattatcgtc
    20701 ggcaggttgc agggaatatc gcaccaccag taaattcgtc aacgacgcgc cgatatccac
    20761 cagcgaatcc accagcgcgg cgagaatact caccgacccg gtataccacc atgcaaaaat
    20821 tttaatcagc aatagcagtg aagccatcgc cgttgcagca atcgccgccc gactgaccag
    20881 ccgtccataa gattgattca taaatactcc cgctatcaac tgacgctagt ataacggaag
    20941 caaatcatct gcaatgcatt aagcagcagg caaattgagg ataaaaaaaa cccccacatc
    21001 atgtggggga agacagggat ggtgtctatg gcaaggaaaa cagggtttac tactgggaac
    21061 gtgagttgct actactcaat agcttcaacg atgaactttt ttgccattgc gtcacgtcac
    21121 gcaactgctc cattcgttgt tgatgtttct catttaaaac cgcttgctgc tccggcgtta
    21181 acaggcgata catttggttg cggacttttg ccatctcaac ctgacgagca atttgctcat
    21241 tcgccatttt ttctgcctgt gcgcgcacag cgttttcatc aaaattttct gcggtgacaa
    21301 ggcgatgcat tgtctccagt tcgctaacat taacaggagg ctgttcgtgc cgggcctgtt
    21361 gcataagatc tcgcatctgc tgacgctgat gttcggttaa acttatgccg tcgaacatat
    21421 ggctctgcgt actgcgctgc gtaagttctt cacccggatg ccagttatcg actgaaccga
    21481 cttcagcagc gtggcttaat gaactgactg ccagcgttga ggccatgacg gcagcggtaa
    21541 ctatgcgcat catttgctcc caaaatcttt ctgtcgcgat tcaacgatag agagtttacg
    21601 attcaggctg caaacatgcg tcagggggtg taaaacaacg taaagtcatg gattagcgac
    21661 gtctgatgac gtaatttctg cctcggaggt atttaaacaa tgaataaaat cctgttagtt
    21721 gatgatgacc gagagctgac ttccctatta aaggagctgc tcgagatgga aggcttcaac
    21781 gtgattgttg cccacgatgg ggaacaggcg cttgatcttc tggacgacag cattgattta
    21841 cttttgcttg atgtaatgat gccgaagaaa aatggtatcg acacattaaa agcacttcgc
    21901 cagacacacc agacgcctgt cattatgttg acggcgcgcg gcagtgaact tgatcgcgtt
    21961 ctcggccttg agctgggcgc agatgactat ctcccgaaac cgtttaatga tcgtgagctg
    22021 gtggcacgta ttcgcgcgat cctgcgccgt tcgcactgga gcgagcaaca gcaaaacaac
    22081 gacaacggtt caccgacact ggaagttgat gccttagtgc taaatccagg ccgtcaggaa
    22141 gccagcttcg acgggcaaac gctggagtta accggtactg agtttaccct gctctatttg
    22201 ctggcacagc atctgggtca ggtggtttcc cgtgaacatt taagccagga agtgctgggc
    22261 aaacgcctga cgcctttcga ccgcgctatc gatatgcaca tttccaacct gcgtcgtaaa
    22321 ctgccggatc gtaaagatgg tcacccgtgg tttaaaacct tgcgtggtcg cggctatctg
    22381 atggtttctg cttcatgata ggcagcttaa ccgcgcgcat cttcgccatc ttctggctga
    22441 cgctggcgct ggtgttgatg ttggttttga tgttacccaa gctcgattca cgccagatga
    22501 ccgagcttct ggatagcgaa cagcgtcagg gtctgatgat tgagcagcat gtcgaagcgg
    22561 agctggcgaa cgatccgccc aacgatttaa tgtggtggcg gcgtctgttc cgggcgattg
    22621 ataagtgggc accgccagga cagcgtttgt tattggtgac caccgaaggc cgcgtgatcg
    22681 gcgctgaacg cagcgaaatg cagatcattc gtaactttat tggtcaggcc gataacgccg
    22741 atcatccgca gaagaaaaag tatgggcgcg tggaactggt cggtccgttc tccgtgcgtg
    22801 atggcgaaga taattaccaa ctttatctga ttcgtccggc cagcagttct caatccgatt
    22861 tcattaactt actgtttgac cgcccgctat tactgctgat tgtcaccatg ttggtcagta
    22921 cgccgctgct gttgtggttg gcctggagtc tggcaaaacc ggcgcgtaag ctgaaaaacg
    22981 ctgccgatga agttgcccag ggaaacttac gccagcaccc ggaactggaa gcggggccac
    23041 aggaattcct tgccgccggt gccagtttta accagatggt caccgcgctg gagcgcatga
    23101 tgacctctca gcagcgtcta ctttctgata tctctcacga actgcgcacc ccgctgacgc
    23161 gtctgcaact gggtacggcg ttactgcgcc gtcgtagcgg tgaaagcaag gaactggagc
    23221 gtattgaaac cgaagcgcaa cgtctggaca gcatgatcaa cgatctgttg gtgatgtcac
    23281 gtaatcagca gaaaaatgcg ctggttagcg agaccatcaa agccaaccag ttgtggagtg
    23341 aagtgctgga taacgcggcg ttcgaagccg agcagatggg caagtcgttg acggttaact
    23401 tcccgcctgg gccgtggccg ctgtacggca acccgaacgc cctggagagt gcgctggaaa
    23461 acattgttcg taatgccctg cgttattccc atacgaagat tgaagtgggc tttgcggtag
    23521 ataaagacgg tatcaccatt acggtggacg acgatggtcc tggcgttagc ccggaagatc
    23581 gcgaacagat tttccgtccg ttctatcgta ccgatgaagc acgcgatcgt gaatctggcg
    23641 gtacaggttt ggggctggca attgttgaaa ccgccattca gcagcatcgt ggctgggtta
    23701 aagcggaaga cagcccgctg ggcggtttac ggctggtgat ttggttgccg ctgtataagc
    23761 ggagttaaac tcggcgcaga gccgtcccct tcggggtgaa ggcaacaggt cagcactgtg
    23821 cgaactgata ccctcacccc ggccctctcc ctgaaagggc gagggggaaa aacgtagccc
    23881 tgttcagcaa cggagctgag caccgctccg gacagtcccc tcgcccctct ggggagaggg
    23941 ttagggtgag gggaaaaatt cagcacaatg caaacggcac tgctctcacc ggaaaccatg
    24001 ccagatttcg tattaaggca gacttatttt ccccacaacc ggcgggaaaa atcttcgatc
    24061 ttgccgctca gtcggcgctt ttgcatcgtc cgcgtccagc ttttcgataa cccggcagcg
    24121 gagagtaaac ggtgatactg ctcgtcatca aacggcatat gccatgcgat ggcggcagcc
    24181 tcctgcacgg tcacatcact gacacgggaa accaactcca gcggagcatc agcagatacc
    24241 aaccctggcg caatcacgct atacaaccac cccactttac ccgtgttttg catcaactgc
    24301 gctatatcgc tgatatcaaa atggtaattg agtttatagc aaggtgagcg tggctggctg
    24361 acctgaatta atgcctctcc ccagcggaaa atatcgccaa tataaacatt actttccgtc
    24421 agaccgtcgg ttgagagatt ttcgccaaac gcaggcgcaa caaacaactc cgcctgttcc
    24481 ggaaattccc gcgcccagta aagataatgc tcacgaggat aatgacacag cgctctgtct
    24541 ggcccgccgt gaaccttttt ctccgcctgc tcgtcacctt ccagccccag ctccgtcagc
    24601 atcaactcac catcaacctg gattttggca attgcgctgg gtttgccttc gggataagcc
    24661 tgaatcttgc ctgtgtatac atcaaccgga tatcgcatct gttccctctc cacgagaaat
    24721 ttccccatta gaccatattt attttatgtc aggcgtgctg ttttcgcggt aagtatccag
    24781 gcaggaactc aatagcaaac cggacgaacg ttagttcatc cggctgtggg gattaaggaa
    24841 gcaggtttat ttcaccgtac ctaaaatttc gattttcgct gctctggctt tgactttacg
    24901 tgaccagata gaggtaagaa ttggcaccag aatcgaagtc acaatgaccg ccgtcgcgac
    24961 cagcgaagtt gctgccggag ccatcggttt aaacgcaggc accatttctg caatcagcac
    25021 aggtgtcgct accgcggccc ctgcggaact ggaagcggca attccggcag tgccatcgcc
    25081 accgccaatc aatttatcag cgataatcaa cggaataccg gtcacgataa ttactgccac
    25141 acccaacagg atccccagca aacctgtctg ggcaattaca gtcaaatcaa tggtattacc
    25201 cagtgcgaag gcaaagaatg gaatcagcgt ctgcaccgct ttgctgaaaa attctcgtaa
    25261 ttcagggtca aggttcccaa gggcaaagcc caccagaaac ggtaatactg cgccgacgaa
    25321 aacatgcggt tcaaacgagg caatcccggc agtgcccaga ataatcatcg tcatgagcgg
    25381 cccggactcc aatgacatca acacaaatgc cccagcttct tcttttgtgc cgtactgctg
    25441 catgatggaa gcgtaaagtc cgccgttggt catatccatc gccgccacca gcgccagcgt
    25501 tgaaagtccg gcaaagaatc caacttcaac accatgttcc ggaataatgc gcgaggcaat
    25561 tgccgcaacc acccacgcga cggcaatttt agttaccacc agcgtaccgg atttacgcaa
    25621 taccgttccc gtcgcgctta attttattga cgcccccatg caaaaaaacc acaccgccag
    25681 aatgggcacc gtaccggtaa tcatcccgtt ggtgaatgat ccaaaatatt tccccgcctc
    25741 cggcgagaag gtgtggcaca gtgcgccaag gaatagcggg acgagcatca tcccccccgg
    25801 gattttctca atcgagcgtt ttatctgcat ttccatcacc tgtcggcata tttttaaggg
    25861 ctgatgcgtc attcctcaat ggagcatcag tgttgatgga aacgaagata gacattcatt
    25921 taatcaataa aagtgatcaa aaccacatca attgaaacac tgttttattt ttatcggatc
    25981 attgttttta ttttgaaatt gatcacaaaa aaaccaccgc cgttggcgat ggttcatttt
    26041 ttaagctgat atgcggcgta aacgcctcat tgcagcaggc ggcaaatgat tattttttcg
    26101 ccgcgaaacg tgccgctgct tcgtcccagt tcaccacgtt ccagaactct ttgatgtagt
    26161 caggacggcg gttctggaat ttcaggtagt aagcatgttc ccacacatcc aggcccagaa
    26221 tcgggaagcc ggaagcgcca gaaatagctt cacccatcag cggggaatcc tggttagcag
    26281 tagaaaccac cgccagttta tcgcctttca gcaccagcca tgcccagccg gaaccaaagc
    26341 gggaagctgc cgctttctca aattctgctt tgaagttatc aacggagccg aagtcacgtt
    26401 cgatagccgc tttcaggtca ccctgtaagg tggtgccttt tttcagacct ttccagaaca
    26461 ggctgtggtt agcgtgaccg ccagcgttgt tgcgcagtac tgttttcttg tctgctggca
    26521 gctggtccag tttggtgatc agctcttcaa ccggcaggtt ggcaaattct ggcaggcttt
    26581 ccagcgccgc gttggcgttg ttgacatagg tctgatggtg tttggtgtgg tggatttcca
    26641 tggtctgctt atcgaagtgc ggttccaggg catcgtaagc atacggcagg gatggcaggg
    26701 tatagctcat attcatctcc agtattgtcg ggcggccgat tgttaatgcc gcgtaagcag
    26761 ttggttcatt atagttaatt aaatgatatt gaaaatgatt atcaatgccg tacttttcgt
    26821 aagggtatgg ttttgcagga aaatgcccga gatgtgaagc aaatcaccca cttaatgccg
    26881 tgattgccag taaatcgaca acggcggcaa caggcgaaag gttaatcgac agcacgattt
    26941 ttacactcat ctcgtcggag atgtgacgcg acgaaaaatg atgaggataa gaagatgagt
    27001 aacgcgatta cgatggggat attttggcat ttgatcggcg cggccagtgc agcctgtttt
    27061 tacgctccgt tcaaaaaagt aaaaaaatgg tcatgggaaa ccatgtggtc agttggtggg
    27121 attgattcgt ggattattct gccgtgggcc atcagcgccc tgttactacc gaatttctgg
    27181 gcgtattaca gctcgtttag tctctctacg ctgctgcctg tttttctgtt cggcgctatg
    27241 tgggggatcg gtaatatcaa ctacggcctg accatgcgtt atctcggcat gtcgatggga
    27301 attggcatcg ccattggcat tacgttgatt gtcggtacgc tgatgacgcc aattatcaac
    27361 ggcaatttcg atgttctgat taataccgaa ggcggacgca tgacgttgct cggcgttctg
    27421 gtggcgctga ttggcgtagg gattgtgact cgcgccgggc agttgaaaga gcgcaagatg
    27481 ggcattaaag ccgaagagtt caacctgaaa aaagggctgg tgctggcggt gatgtgcggc
    27541 attttctctg ccggaatgtc ctttgcgatg aacgccgcaa aaccgatgca tgaagccgct
    27601 gccgcacttg gcgtcgatcc actgtatgtc gctctaccaa gctatgttgt catcatggga
    27661 ggcggcgcga tcatcaacct cggtttctgc ttcattcgtc tggcaaaagt gaaggatttg
    27721 tcgctaaaag ccgacttttc gctggcaaaa ccgctgatca ttcacaatgt gttactctca
    27781 gcactgggcg ggttgatgtg gtatctgcaa ttctttttct atgcctgggg ccacgcgcgt
    27841 attccggcgc agtatgacta catcagctgg atgctgcata tgagcttcta tgtattgtgc
    27901 ggcggtatcg tcgggctggt actgaaagag tggaacaatg caggtcgccg tccggtaacg
    27961 gtgttgagcc tcggttgtgt ggtgattatt gtcgccgcca acatcgtcgg catgggcatg
    28021 gcgaattaat ctttctgcga attgagatga cgccagtggc tgggcgtcat cccggtttcc
    28081 cgggtaaaca ccaccgaaaa atagttacta tcttcaaagc cacattcggt tgaaatatca
    28141 ctgattaaca ggcggctatg ctggagaaga tattgcgcat gacacactct gatctgccgc
    28201 agatattgat tgatggtcat gccagtctgc tggcgaaatt gctgacgcaa aacgcgctca
    28261 ctgcacgatg cctcatcaca aaatttatcc agcgcaaagg gacttttcag gctggccgcc
    28321 agccgggtaa tcagcttatc cagcaatgtt tcgctggatg ttggcggcaa cgaatcgctg
    28381 gtgtaacgat ggcgattcag caacatcacc aactgcccga acagcaactc tgccatttcg
    28441 ttagcaaacg gcacatgctg actactttca tgctcaagtt gaccgataac ctgccgcgcc
    28501 tgcgccatcc ccacgctacc taagcgccag tgtggttgcc ctgcgctggc actaaatccc
    28561 ggaatcgccc cctgccagtc aagattcagc ttcagccgct ccgggcaata aataatattc
    28621 tgcaaaacca gatcgttaac ggaagcgtag gagtgtttat cgtcagcatg aatgtaaaag
    28681 agatcgccac gggtaatgcg ataagggcga tcgttgagta catgcagacc attgccgcgc
    28741 cagacaatta ccagctcaca aaaatcatgt gtatgttcag caaagacatc ttgcggataa
    28801 cggtcagcca cagcgactgc ctgctggtcg ctggcaaaaa aatcatcttt gagaagtttt
    28861 atctgatgcg ccaccgtggc tacctcggcc agagaacgaa gttgattatt cgcaatatgg
    28921 cgtacaaata cggtgagtag attcgcgtta ttgcaaaaag ccatcccgtc cctgacgaat
    28981 atcacgcggt gaccagttaa actctcggcg aaaaagcgtc gaaaagtggt tactgtcgct
    29041 gaatccgcag cgataggcga tgtcagtaac gctggcctcg ctgtggcgta gcagatgtcg
    29101 ggctttcatc agtcgcaggc ggttcaggta acgctgaggc gtcagtcccg tttgctgctt
    29161 aagctgccga tgtagcgtac gcagtgaaag agaaaattga tccgccacgg catcccaatt
    29221 cacctcatcg gcaaaatggt cctccagcca ggccagaagc aagttgagac gtgatgcgct
    29281 gttttccagg ttctcctgca aactgctttt acgcagcaag agcagtaatt gcataaacaa
    29341 gatctcgcga ctggcggtcg agggtaaatc attttcccct tcctgctgtt ccatctgtgc
    29401 aaccagctgt cgcacctgct gcaatacgct gtggttaacg cgccagtgag acggatactg
    29461 cccatccagc tcttgtggca gcaactgatt cagcccggcg agaaactgaa atcgatccgg
    29521 cgagcgatac agcacattgg tcagacacag attatcggta tgttcataca gatgccgatc
    29581 atgatcgcgt acgaaacaga ccgtgccacc ggtgatggta tagggctgcc cattaaacac
    29641 atgaataccc gtgccatgtt cgacaatcac aatttcatga aaatcatgat gatgttcagg
    29701 aaaatccgcc tgcgggagcc ggggttctat cgccacggac gcgttaccag acggaaaaaa
    29761 atccacacta tgtaatacgg tcatactggc ctcctgatgt cgtcaacacg gcgaaatagt
    29821 aatcacgagg tcaggttctt accttaaatt ttcgacggaa aaccacgtaa aaaaagtcga
    29881 tttttcaaga tacagcgtga attttcagga aatgcggtaa gcatcacacc accacaattc
    29941 agcgaattgt gaacatcatc acgttcatct ttccctggtt gccaatgacc cattttcctg
    30001 tcagtaacga gaaggtcgcg aagtcaggcg ctttttagac tggtcgtaat gaaattcagc
    30061 aggatcacat tatgaccttt cgcaattgtg tcgccgtcga tctcggcgca tccagtggac
    30121 gcgtgatgct ggcgcgttac gagcgtgaat gccgcagcct gacgctgcgc gaaatccatc
    30181 gttttaacaa tgggctgcat agccagaacg gttatgtcac ctgggatgtg ggtagcctgg
    30241 aaagtgccat tcgccttgga ttaaacaagg tgtgcgagga agggattcgt atcgatagca
    30301 ttgggattga tacctggggc gtggactttg tgctgctcga ccaacagggt cagcgtgtgg
    30361 gcctgcccgt tgcttatcgc gatagccgca ccaatggcct gatggcgcag gcacaacaac
    30421 aactcggcaa acgcaatatt tatcaacgta gcggcatcca gtttctgccc ttcaatacgc
    30481 tttatcagtt gcatgcactg acagagcaac aacctgagct tattccacac attgctcacg
    30541 ctctgctgat gccggattac ttcagttatc gcctgaccgg cgagatgaac tgggagtaca
    30601 ccaacgccac gaccacgcaa ctggtcaata tcaatagcga cgactgggac gagtcgctac
    30661 tggcgtggag cggtgccaac aaagcctggt ttggtcgccc gacgcatccg ggtaatgtca
    30721 taggtcactg gatttgcccg cagggtaatg agattccggt ggtcgccgtt gccagccatg
    30781 ataccgccag cgcggttatc gcctcgccgt taaacggttc acgcgccgct tatctctctt
    30841 ctggcacctg gtcattgatg ggcttcgaaa gccagacgcc atttaccaat gacacggcgc
    30901 tggccgccaa tatcaccaat gaaggtgggg cggaaggtcg ctatcgggtg ctgaaaaata
    30961 ttatgggctt atggctgctt cagcgggtgc ttcaggagcg gcaaatcaac gatctcccgg
    31021 cgcttatcgc cgcgacacag gcactgccgg cttgccgctt caccatcaat cccaatgacg
    31081 atcgctttat taatcctgag gcgatgtgca gcgaaattca ggctgcgtgt cgggaaacgg
    31141 cgcaaccaat cccggaaagt gatgctgaac tggcgcgctg tattttcgac agtctggcgt
    31201 tgctgtatgc cgatgtgttg catgagctgg cgcagctgcg cggtgaagat ttctcgcaac
    31261 tgcatattgt cggcggtggc tgccagaacg cgctgctcaa ccagttatgt gctgatgcct
    31321 gcggtattcg ggtgatcgcc gggcctgttg aagcctcgac gctcggcaat atcggcatcc
    31381 agttaatgac gctggatgaa ctcaacaatg tggatgattt ccgtcaggtc gtcagcacca
    31441 ccgcgaatct gaccaccttt acccctaatc ctgacagtga aattgcccac tatgtggcgc
    31501 agattcactc tacacgacag acaaaggagc tttgcgcatg accactcaac tggaacaggc
    31561 ctgggagctg gcgaaacagc gtttcgcagc ggtagggatt gatgtcgagg aggcgctgcg
    31621 ccaacttgat cgtttacccg tgtcaatgca ctgctggcaa ggcgatgatg tttccggttt
    31681 tgaaaacccg gaaggttcgc tgaccggggg gattcaggcc acaggtaatt atccgggtaa
    31741 agcgcgtaat gccagcgagc tgcgtgccga tctggaacag gctatgcggc tgattccggg
    31801 gccgaaacgg cttaatttac atgccatcta tctggaatca gacacgccag tctcgcgcga
    31861 ccagatcaaa ccagagcact tcaaaaactg ggttgaatgg gcgaaagcca atcagctcgg
    31921 tctggatttt aacccctcct gtttttcgca tccgctaagc gccgatggct ttacgctttc
    31981 ccatgccgac gacagcattc gccagttctg gattgatcac tgcaaggcca gccgccgcgt
    32041 ttcggcctat tttggcgagc aacttggcac gccatcggtg atgaacatct ggatcccgga
    32101 tggcatgaaa gatatcaccg ttgaccgtct cgctccgcgc cagcgtttgc tggcagcact
    32161 ggatgaggtg atcagcgaga agctggatcc ggcgcaccat atcgacgccg ttgagagcaa
    32221 attatttggt attggtgcgg agagctacac ggttggctcc aatgagtttt acatggggta
    32281 tgccaccagc cgccagacgg cgctgtgcct ggacgccggg catttccacc cgactgaagt
    32341 gatttccgac aagatttccg ccgccatgct gtatgtgccg cagttgctac tgcacgtcag
    32401 tcgtccggtt cgctgggaca gcgatcacgt ggtgctgctg gatgatgaaa ctcaggcgat
    32461 tgccagtgag attgttcgtc acgatctgtt cgaccgtgtg catatcggtc tcgatttctt
    32521 tgatgcctct atcaaccgca ttgccgcgtg ggtcattggt acacgcaata tgaaaaaagc
    32581 cctgctgcgt gcgttgctgg aacctaccgc tgagctgcgc aagctggaag cggcgggcga
    32641 ttacactgcg cgtctggcac tgctggaaga gcagaaatcg ttgccgtggc aggcggtctg
    32701 ggaaatgtat tgccaacgtc acgatacccc agcgggtagc gaatggttga agagcgtgcg
    32761 ggcttatgag aaagaaattt tgagtcgccg cgggtaaaca ctgccggatg cggcgcgagc
    32821 gccttattcg gcctacgggt cgtgcatccg acaacaccga atttacagga acacagaaca
    32881 tgcaaaacat tacccagtcc tggtttgtcc agggaatgat caaagccacc accgacgcct
    32941 ggctgaaagg ctgggacgag cgcaatggtg gtaacctgac actgcgcctg gatgacgccg
    33001 atatcgcacc gtatcacgac aacttccacc aacaaccgcg ctatatcccg ctcagccagc
    33061 ccatgccttt actggcaaat acaccgttta ttgtcaccgg ctccggcaaa ttcttccgca
    33121 acgtccagct tgatcctgcg gctaacttag gcgtcgtaaa agtcgacagc gacggcgcgg
    33181 gctaccacat tctctggggg ctaactcacg aagccgttcc aacctctgaa ctcccggcgc
    33241 acttcctctc ccactgcgaa cgcattaaag ccaccaacgg taaagatcgg gtgatcatgc
    33301 actgccatgc caccaacctg atcgccctta cctacgtact ggaaaacgac accgcggtct
    33361 tcactcgcca actgtgggaa ggcagcaccg aatgtctggt agtgttcccg gatggcgtcg
    33421 gcattttgcc gtggatggtg cccggcacgg acgaaatcgg ccaggcgacc gcacaggaaa
    33481 tgcaaaaaca ttcgctggtg ttgtggccct tccacggcgt cttcggcagc ggtccgacac
    33541 tggatgaaac attcggttta atcgacaccg cagaaaaatc agcacaagta ttagtgaagg
    33601 tttattcgat gggcggcatg aaacagacca tcagtcgtga agagctgata gcgctcggcc
    33661 agcgtttcgg cgttacacca ctcgccagtg cgctggcgct gtaaggagca aacatgatcc
    33721 gcaaagcctt tgtaatgcag gttaaccccg acgcccacga agagtatcag cgtcggcata
    33781 accccatctg gccggaactg gaagcagtgt tgaaatctca cggtgcgcat aactacgcca
    33841 tttacctcga caaagcgcgt aatctgctgt ttgccacggt agagattgaa tctgaagaac
    33901 ggtggaatgc ggtagccagc actgatgttt gccaacgttg gtggaaatat atgaccgacg
    33961 ttatgccagc taacccggat aacagcccag tcagtagcga gctgcaagaa gtgttttacc
    34021 tgccctaatg agttgcggtg ccggaaaata tccagcaccg cgccttcatc agaagaagta
    34081 tttaaagcga acgcgtgcgc cgtaacggtc atcagtatca tcaccgtgtt ttttatcacc
    34141 gtcgctatcc agaatggagt aatacgtccc gaggtaaata ttaaagttct ccatatccat
    34201 cacattagcg aactgataag acgcatgaat ggtgtgaatg gtgtacgtcc cttcatcatc
    34261 aatccagcag tcgttatcac aaactacacc gctgaattca tcaatcttat tgtgcgcgta
    34321 gatataaccc agctcgaaac gtttccacag cgcgttaatc ccggcagtga aatctttctc
    34381 attattagca tctaaatagg cggtattaag attaacaacg acgccatttt ccggatccgt
    34441 tttcaggcca ttccaggtca tgctcatacc atagccggta cgatcggact gatcgacaaa
    34501 attacccttg ctatcggtat aaccataagc attatttacc acgttcgctt ccatcgctgc
    34561 ggaaacggta aattcttccg tcggcgacca ggcaattacc ggacgcagat aagcaacatt
    34621 tttctgctgc tccacatcgc gtccatgata attaccgtca ttatataaag atgttccgtc
    34681 ttcaagtaac gtgtttaatt caaaatacaa gttatcaagt tgtttgctga cgaggaaatt
    34741 accgccagcg ttagagcgcc cgcggccctc tttcatcata tagatataac cgctgccatc
    34801 gtcataaaga tcgttcgcag tattaccgga atgttcaaca aaggtatcct gattcagcgg
    34861 gaacatatcg taggcttcaa aacggcctac tttgaccttc cagtcgtttt gtttgccaaa
    34921 gaagaatacc gcatcatcga tatttactga accgtgcatg tcccccagcg gttgtgcgga
    34981 gaacccggcg aaatagccat tatccagttt tcgcatacca tcaaaaccta acagaatacg
    35041 accattgaga ttccactgtt cattagtggg atcgctattc acatcagcgt tggtcattgc
    35101 cagcaggcca tgattacttt ccgcatccat attaaattct acatcgccgt agatttttaa
    35161 atcgccataa cctgagagca aaagattagg tgaggtggaa tttttctttg cagattccgc
    35221 cgttgcctgt ccatttaccg gcgtattgcc ctgggcagcc ttaatttcgc ggatctcagc
    35281 ggcttgttgt gtctgcagct gtttaacctg gctctctgct tttgctgccc gcatttctgc
    35341 cgcttccagg cgttcttcca gcaatgccat gcgttgttcc agcgttaaat gctgtgtttt
    35401 tgccattccg gtactacatg ccaataataa accgatggct aaagataatg tcgatttttt
    35461 cattatttac gttccctgaa ataaaagaga ccctacgctt tccaaaagga atacgtatat
    35521 agagaaatat aaggtgtgca ctttcgaatt tttaatccaa cttcagcagg ccgatggata
    35581 gtatcccgct gcataatatg agtaaatccg ctgatttcct ggaacgtgag gaatataaca
    35641 atagtgccgt ttttaactaa atatcgtaaa aaatattaga tataattcac gctgactcat
    35701 aagccacaaa tataaataac cttgctgcaa aacatg
//
LOCUS       DACBVI010000033        35470 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.033, whole genome shotgun sequence.
ACCESSION   DACBVI010000033 DACBVI010000000
VERSION     DACBVI010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.033"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            268..2973
                     /gene="malT"
                     /locus_tag="IU325_07970"
     CDS             268..2973
                     /gene="malT"
                     /locus_tag="IU325_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HAP5808968.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALINRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(3016..4032)
                     /gene="rtcA"
                     /locus_tag="IU325_07975"
     CDS             complement(3016..4032)
                     /gene="rtcA"
                     /locus_tag="IU325_07975"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HAP5808969.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVHGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFICRVLEPLLAKMGIHQQTTLLRHGFY
                     PAGGGVVAAEVSPVALFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLVASFSL
                     HEQNIHSLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFSLVEADGVTR
                     VSIE"
     gene            complement(4036..5262)
                     /locus_tag="IU325_07980"
     CDS             complement(4036..5262)
                     /locus_tag="IU325_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HAP5808970.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSVTQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            5449..7047
                     /locus_tag="IU325_07985"
     CDS             5449..7047
                     /locus_tag="IU325_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP5808971.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYAFQ
                     PENEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEMNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLLQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTQLLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEALQDQHSSS"
     gene            complement(7029..7787)
                     /locus_tag="IU325_07990"
     CDS             complement(7029..7787)
                     /locus_tag="IU325_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HAP5808972.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(7804..8634)
                     /gene="glpG"
                     /locus_tag="IU325_07995"
     CDS             complement(7804..8634)
                     /gene="glpG"
                     /locus_tag="IU325_07995"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HAP5808973.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADSRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(8679..9005)
                     /gene="glpE"
                     /locus_tag="IU325_08000"
     CDS             complement(8679..9005)
                     /gene="glpE"
                     /locus_tag="IU325_08000"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HAP5808974.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            9195..10700
                     /locus_tag="IU325_08005"
     CDS             9195..10700
                     /locus_tag="IU325_08005"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HAP5808975.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYSARLRRRYPFLTESLARHYARTYGSNSELLLCNAGAIGDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQRLSLAS"
     gene            complement(10915..11515)
                     /locus_tag="IU325_08010"
                     /pseudo
     CDS             complement(10915..11515)
                     /locus_tag="IU325_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033835.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11518..12510)
                     /locus_tag="IU325_08015"
     CDS             complement(11518..12510)
                     /locus_tag="IU325_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP5808976.1"
                     /translation="MNKFTCVLLLGTALLAGCVDREGYYNSVREEDSHGLTSLRGQPE
                     LRYNDDWSRWPRVYGATALYPLYASAYYELVPEPKDKDRTSLAWQAYGLQQTRTAEAY
                     DSLIKGTATVIFVAQPSEGQKKRAEEAGVKLKYTAFAREAFVFIVDIKNPVNSLSEQQ
                     VKDIFSGKVSRWNKVGGGDEGIKIWQRPEDSGSQTVMKGLVMQDTPMLPAKKSTVIDL
                     MGGLITEVADYQNTPSSIGYTFHYYVTRMNDNMLKMRKQIKLLAINGVAPTEENIRNG
                     TYPYIIHAYMVTHENPTLETQKFVDWFLSPQGQQLVEDVGYVPIYDASSESSGQ"
     gene            complement(12536..14044)
                     /locus_tag="IU325_08020"
     CDS             complement(12536..14044)
                     /locus_tag="IU325_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP5808977.1"
                     /translation="MMQNGKWILTSLVMTFFGIPILAQFLAAVVAMLGAGLAAILEFC
                     NLLFTPTIYLLLNVFMLTLGALLLFFSGRVWAGDSAPENREIAAWRQCLFLVPALLTL
                     VGWIITLHLADYQFRQMGAGWLANLMLPWLGVFTVSFVGGEYWWIVIIPVGAHISFSL
                     GYGWPTRHPLTGTSGLRCRNLLLFILLLLGIVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHVWDYLD
                     NSRTQEAYNKIVNGDADIIFVAQPSDGQKKRAEKSGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYVVDAFMVTRDNMTSETQKLVEWFLTPQGQSLVEDVGYVPLYPTMK"
     gene            complement(14170..16617)
                     /locus_tag="IU325_08025"
     CDS             complement(14170..16617)
                     /locus_tag="IU325_08025"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HAP5808978.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYERQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(16636..18069)
                     /gene="glgA"
                     /locus_tag="IU325_08030"
     CDS             complement(16636..18069)
                     /gene="glgA"
                     /locus_tag="IU325_08030"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HAP5808979.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(18069..19364)
                     /gene="glgC"
                     /locus_tag="IU325_08035"
     CDS             complement(18069..19364)
                     /gene="glgC"
                     /locus_tag="IU325_08035"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HAP5808980.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(19382..21355)
                     /gene="glgX"
                     /locus_tag="IU325_08040"
     CDS             complement(19382..21355)
                     /gene="glgX"
                     /locus_tag="IU325_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HAP5808981.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCIFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(21352..23538)
                     /gene="glgB"
                     /locus_tag="IU325_08045"
     CDS             complement(21352..23538)
                     /gene="glgB"
                     /locus_tag="IU325_08045"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HAP5808982.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNVGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(23811..24914)
                     /gene="asd"
                     /locus_tag="IU325_08050"
     CDS             complement(23811..24914)
                     /gene="asd"
                     /locus_tag="IU325_08050"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP5808983.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYSHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            25106..25699
                     /gene="yhgN"
                     /locus_tag="IU325_08055"
     CDS             25106..25699
                     /gene="yhgN"
                     /locus_tag="IU325_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HAP5808984.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(25745..27040)
                     /locus_tag="IU325_08060"
     CDS             complement(25745..27040)
                     /locus_tag="IU325_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HAP5808985.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPADLLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTADLRYTQQLAAKLAQQGICVISGGARGIDECAIASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVLVKPNQDVQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPSHTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISMWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(26947..29043)
                     /locus_tag="IU325_08065"
     CDS             complement(26947..29043)
                     /locus_tag="IU325_08065"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HAP5808986.1"
                     /translation="MEKHGAELLLQRMLGNTTATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLQGEVDCLLVSPERLANQDFIETVLYPIADRIGLLVVDEAHCISDWGHDF
                     RPDYRRILDILRQLPANTPVLGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDA
                     LVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLRKNGISAFAYYSGVTCE
                     GAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQV
                     GRAGRAIDSAVGILLCGGEDRAIHQFFRESAFPAEAQIHEILNVLSENDGLTLRGIEQ
                     RTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELAD
                     VESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPDIDSGLLHAANLFIKHA
                     DLPLNLNKQVAAGAFTQYGFKGNLPAGLQGSTGRILSRWGDSGWGKQVAQEKKTGRLS
                     DELVEACAEMVRQRWNPHPEPTWVCCVPSLRHLDLVPDFARRLAAKLGLPFIDAIEKV
                     VDNPPQKMQQNRFHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQAG
                     CPTVYPLALASTSVKN"
     gene            complement(29183..30523)
                     /gene="gntU"
                     /locus_tag="IU325_08070"
     CDS             complement(29183..30523)
                     /gene="gntU"
                     /locus_tag="IU325_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAP5808987.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(30527..31054)
                     /gene="gntK"
                     /locus_tag="IU325_08075"
     CDS             complement(30527..31054)
                     /gene="gntK"
                     /locus_tag="IU325_08075"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP5808988.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(31193..32188)
                     /locus_tag="IU325_08080"
     CDS             complement(31193..32188)
                     /locus_tag="IU325_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GntR"
                     /protein_id="HAP5808989.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            32266..32415
                     /locus_tag="IU325_08085"
     CDS             32266..32415
                     /locus_tag="IU325_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5808990.1"
                     /translation="MFTIIRYTLNQLHRIVLTTRQNPGSVHQHATGASEEACKKAGFK
                     DAVEF"
     gene            complement(32412..33107)
                     /locus_tag="IU325_08090"
     CDS             complement(32412..33107)
                     /locus_tag="IU325_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP5808991.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVYQDM
                     ELYRWALVKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(33230..34267)
                     /locus_tag="IU325_08095"
     CDS             complement(33230..34267)
                     /locus_tag="IU325_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP5808992.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITNGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            34602..35090
                     /locus_tag="IU325_08100"
     CDS             34602..35090
                     /locus_tag="IU325_08100"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP5808993.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFIDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            35326..>35470
                     /locus_tag="IU325_08105"
     CDS             35326..>35470
                     /locus_tag="IU325_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HAP5808994.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSF
                     LNSI"
BASE COUNT         8837 a         9550 c         8698 g         8385 t
ORIGIN      
        1 tttcatcagg aatgaagaag aaacccttgc ttaaatgaat ctgatgaaca taagggaaat
       61 accgtacaac ccagcattca cgctgtatca gcgtcgtttt aggtgagtca ttaataaaga
      121 tttggaattg tgacagagtg caaattcaga cacataaaaa aacgtcatcg cttgcattag
      181 aaaggtttct ggccgacctt ataaccatta attacgaagc gcaaaaaaaa taatatttcc
      241 tcattttcca cagtgaagtg attaactatg ctgattccgt caaaattaag tcgtccggtt
      301 cgactcgacc ataccgtggt tcgtgagcgc ctgctggcta aactttccgg cgcgaacaac
      361 ttccggctgg cgctgatcac aagtcctgcg ggctacggaa agaccacgct catttcccag
      421 tgggcggcag gcaaaaacga tatcggctgg tactcgctgg atgaaggtga taaccagcaa
      481 gagcgtttcg ccagctatct cattgccgcc gtgcagcagg caaccaacgg tcactgtgcg
      541 atatgtgaga cgatggcgca aaaacggcaa tatgccagcc tgacgtcact cttcgcccag
      601 cttttcattg agctggcgga atggcatagc ccactttatc tggtcatcga tgactatcat
      661 ctgatcacta atccagtgat ccacgagtca atgcgcttct ttattcgcca tcaaccagaa
      721 aatctcaccc tggtggtgtt gtcacgcaac cttccgcaac tgggcattgc caatctgcgt
      781 gttcgtgatc aactgctgga aattggcagt cagcaactgg catttaccca tcaggaagcg
      841 aagcagtttt ttgattgccg tctgtcatcg ccgattgaag ccgcagaaag cagtcggatt
      901 tgcgatgacg tttccggttg ggcgacggca ctacagctaa tcgccctctc cgcccggcag
      961 aatacccact cagcccataa gtcggcacgc cgcctggcgg gaatcaatgc cagccatctt
     1021 tcggattatc tggtcgatga ggttttggat aacgtcgatc tcgcaacgcg ccattttctg
     1081 ttgaaaagcg ctattttgcg ctcaatgaac gatgcactca tcaaccgtgt gaccggcgaa
     1141 gaaaacgggc aaatgcgcct cgaagagatt gagcgccagg ggctgttttt acagcggatg
     1201 gatgataccg gcgagtggtt ctgctatcac ccgctgtttg gtaacttcct gcgtcagcgc
     1261 tgccagtggg aactggcggc ggagctgccg gaaattcacc gtgctgccgc agaaagctgg
     1321 atggcccagg gatttcccag cgaagcgatt catcatgcgc tggcggcagg cgatgcgctg
     1381 atgctgcgcg atattctgct taatcacgcc tggagtctgt tcaaccatag cgaactgtcg
     1441 ctgctggaag agtcgcttaa ggccctgccg tgggacagtt tgctggaaaa tccgcagtta
     1501 gtgttattgc aggcgtggct gatgcaaagc caacatcgct acggcgaagt taacaccctg
     1561 ctagcccgtg ctgaacatga aatcaaggac atcagagaag gcaccatgca cgcagaattt
     1621 aacgctctgc gcgcccaggt agcgattaac gatggtaatc cggatgaagc ggaacggctg
     1681 gcaaaactgg cactggaaga actgcctccg ggctggttct atagccgcat tgtagcaacc
     1741 tcagtgctgg gtgaagtatt gcactgcaaa ggcgaattga cccgctcact ggcgttaatg
     1801 cagcaaaccg aacagatggc gcgccagcac gatgtctggc actacgcctt atggagttta
     1861 atccagcaaa gtgaaattct gtttgcccaa gggttcctgc aaaccgcgtg ggaaacgcag
     1921 gaaaaagcgt tccagctgat caacgagcag catctggaac agctgccaat gcatgagttt
     1981 ctggtgcgca ttcgtgcgca gctgttatgg gcctgggcgc ggctggatga agccgaagcg
     2041 tcggcgcgta gcgggattga agtcttgtcg tcttatcagc cacagcaaca gcttcagtgc
     2101 ctggcaatgt tgattcaatg ctcgctggcc cgtggtgatt tagataacgc ccgtagccag
     2161 ctgaaccgtc tggaaaacct gctgggaaat ggcaaatatc acagcgactg gatctctaac
     2221 gccaacaaag tccgggtgat ttactggcaa atgaccggcg ataaagccgc cgctgccaac
     2281 tggttgcgtc atacggctaa accagagttt gcgaacaacc acttcctgca aggtcaatgg
     2341 cgcaacattg cccgtgcaca aatcttgctg ggcgagtttg aaccggcaga aattgttctc
     2401 gaagaactca atgaaaacgc ccgcagtctg cggttgatga gcgatctcaa ccgtaacctg
     2461 ttgctgctta atcaactgta ctggcaggcc ggacgtaaaa gtgacgccca gcgtgtgttg
     2521 ctggacgcat taaaactggc gaatcgcacc ggatttatca gtcattttgt catcgaaggc
     2581 gaagcgatgg cgcaacaact gcgccagctg attcaactca atacgctgcc ggaactggaa
     2641 cagcatcgtg cgcagcgtat tctgcgagaa atcaatcaac atcatcggca taaattcgcc
     2701 catttcgatg agaatttcgt tgaacgtctg ctaaatcatc cggaagtgcc agaactgatc
     2761 cgcaccagcc cgttgaccca acgtgaatgg caggtactgg ggctgattta ctccggttac
     2821 agcaacgagc aaattgcagg cgagctggaa gtggcggcaa ccaccatcaa aacgcatatc
     2881 cgcaatctgt accaaaaact cggtgtcgcc caccgtcagg ccgcggtaca acacgcgcag
     2941 aaactgctga agatgatggg atatggggta tgagttcagc cggatattac gtcggatccg
     3001 gcttacatct ttatatcatt caatgctcac ccgcgttacg ccatcagcct ccaccagact
     3061 aaaccgcact ggcaagaaac gctccaccac cgcgatattg gtcagcagat ggcatgaggg
     3121 atgggcgacc gtaaattctc ccgcgcccgc cagcgccatc ggtagcacca gctggtcggc
     3181 gagatattcc cccactgccg ccgggcttgc caggtagcgt ttcacctctt tcactaactg
     3241 cgcggctacc acttcggcac tgacgcgctt ttcaccgacg acgaaaaagc gttcagtgat
     3301 attttcactt tcgacttcca gcgagacggt attacccggc ccctggtcgc gcggcaggct
     3361 atgaatattc tgctcatgca gggaaaaact tgccaccagc gtagcgattt cacgctcagc
     3421 aacatgcctc ggcacgccag ctaatagcac ctctccacgc atctgcacaa tgttcccgcg
     3481 ctcgccaagt tgcaaggtgt taaacaatgc cacgggcgag acttccgctg ccaccacccc
     3541 gcctccagca ggataaaaac cgtggcgtaa taatgtggtt tgctgatgaa tgcccatttt
     3601 cgccagcagc ggctccagca cccgacagat aaaatccgca ggcggtgccg acgggttatc
     3661 ggtgccaccg ctcacttcaa cacgcgaagg tccatcagca aaccacagcg cgggcagcac
     3721 cgtttgcagt accagcgtac aacttccggc gctaccgata gcaaagcggt aatcgccgcc
     3781 gtgcacggta cctggccgga agaccagacg ctgcgaccct agctccgcgc cttccaccgt
     3841 tgccctacaa atttccgcag ccgcttttac cgcggtcaga tgctggcgca acagccccgg
     3901 tttcgcccgc ccggcacgaa tgccagtgat ggtaaatggc tggccagtta tcatcgacag
     3961 gctcagcgcc gagcgcagga tctgcccgcc gccttcgccc tgtgcgccat ccagcgcaat
     4021 catccttttc atcatttatc cttttacgca caccacctga cgcagggtat agataacttc
     4081 caccagatcg ctttgtgccg ccatcaccgc atcaatatct ttatacgcca tcgggatttc
     4141 gtcgatcact tcggcatctt tacggcattc cacatgcgcg gtggcacgaa tttgatcttc
     4201 cacgctgaac agttttttcg ctttggtacg gctcattacc cgcccggcac cgtggctgca
     4261 cgaacagaac gattcttcat ttcccagccc acggacgata aagcttttcg ctcccatcga
     4321 ccctggaata attccatatt gacccgcacg cgcagacacc gcgcctttac gcgtcacgta
     4381 gatctcttca ccaaagtgct gttctttttg cacatagttg tgatgacagt tgatctcttc
     4441 catcgccagc gtttgtggct gtcttaccgt tttctgcgta acgctctgca acgccgttac
     4501 cacgttttcc atcatcgcat cgcggttaag gctggcaaaa agctgcgccc aggccacggc
     4561 tttcaggtaa tcatcaaagt attccgtacc ttccataaag tacgccagat cacgcgacgg
     4621 caacgtctca agttggtcct gcatctcttt ttgcgccaga tcgataaagt aagtcccgat
     4681 ggcgttacca attccgcgtg aaccggagtg cagcataatc cacacctggt ccgactcatc
     4741 aaggcagatt tcaataaagt ggttacccgt tcccagcgtt cccaggtgtt tatagttatt
     4801 ggtattcagg aaacggggat atttttgcgt taaccactga taaccggctt ccagctcagc
     4861 ccatttcgca tcgacgttaa caggcggatt ttcccatgcg cctttatcac gtttacaacg
     4921 tccggtagta cgtccgtgcg gcacggctgt ttcaatcgcc tgacgcagct ccgccaggtt
     4981 ttcaggcagg tctgccgccg ttaacgcggt acgcagcgcg ttcattccac aaccgatatc
     5041 cacgcccacc gccgccggaa taatcgcccc tttggttggg atcacgctac caatggtgga
     5101 acctttaccc aggtgtacat caggcattac cgcaatatgt ttgaaaataa acggcatctt
     5161 cgccgtatta ataagttgct gacgcgcatc ggcctctacc ggcacgcctt tggtccacat
     5221 ttttaccggg gcattttcag tggtcagtaa ttcgtaattc attttgtttt tctcttttcg
     5281 ttggtatgtc ctgataattg caaccgtcgt accagaaaat taaataagca gcttaatttt
     5341 ttaattcatt gtttttaaaa aaattatacc tttacgtccg taaacggaga tttcccgcaa
     5401 agccaattta ccgataatga aatatcgtct tttataagga tatctaagat gcgtaaaaca
     5461 gtggcttttg gttttgtcgg aacggtgctg gattatgccg ggcgcggcag tcagcgctgg
     5521 tcaaaatggc gtccgacact ctgtctatgc cagcaagaat cgttagttat cgatcggctg
     5581 gaattattgc acgacgcccg ctcgcgctcg ctattcgaaa cgcttaaacg cgatatcgcc
     5641 agcgtttcgc cagaaacaga agtggtgggc gttgagatag aactgcataa cccgtgggat
     5701 ttcgaagagg tttacgcctg cctgcatgat ttcgcccgtg gttacgcgtt ccagccagaa
     5761 aatgaagatt atttaattca catcaccacc ggcacccacg tcgcgcagat ttgctggttt
     5821 ctactggcag aagcgcgtta cctgcccgcc cggttgatac aatcttcacc accgcgcaaa
     5881 aaagaacaac cgcgcggcgc aggtgaagtg acgattatcg atctcgattt aagccgttat
     5941 aacgctatcg ccagccgctt tgccgaggaa cgccaacaaa cgctcgattt cctcaaatca
     6001 ggcatcgcca cgcgtaaccc acacttcaac cgcatgattg agcagatcga aaaagtggcg
     6061 atcaaatccc gcgcaccgat cctgcttaac ggcccgaccg gggcgggtaa gtcatttctg
     6121 gcgcgacgca tctttgaatt gaaacaggcg cggcatcagt ttagcggcgc gtttgtggaa
     6181 atgaactgcg ccaccctgcg tggcgatacc gccatgtcga cgctgtttgg tcatgtgaaa
     6241 ggcgcgttta ccggggcgcg ggagtcgcgg gaaggattat tacgcagcgc caacggcggg
     6301 atgttatttc ttgatgagat tggcgaattg ggcgcagacg aacaggcaat gctgctgaaa
     6361 gccattgaag agaaaacctt ttatccgttt ggcagcgatc gccaggtgag tagcgacttt
     6421 cagcttatcg ccggaacggt gcgcgatttg cgtcagctgg ttgccgaagg caaattccgc
     6481 gaagatctgt acgcgcggat caatctctgg accttcaccc tgccgggtct actccagcgc
     6541 caggaagata ttgaaccgaa cctggattat gaagtggagc gccacgcctc actcactggt
     6601 gacagcgtgc gttttaacac cgaagcgcgg cgcgcctggc tggcctttgc gacatctccc
     6661 caggcaacat ggcgcggtaa ctttcgcgaa ctttctgcca gcgtcacgcg gatggccacc
     6721 tttgccacta gcggacgcat cactctggac gtggttgaag atgagataaa ccgtctgcgc
     6781 tataactggc aggagagccg tccctccgcg cttacacaac tgctgggcgc agaggcagaa
     6841 aacatcgatc tcttcgaccg tatgcaactg gaacacgtta tcgctatctg ccgccaggca
     6901 aagtcgcttt ctgctgccgg acgccagctt tttgacgttt cgcgccaggg caaagccagc
     6961 gtcaacgacg cagatcggct acgcaaatac ctggcgcgtt ttggtctgac gtgggaagcc
     7021 ttgcaggatc agcacagctc cagttgaata tgatggtccg tcagcacctg catgacgctt
     7081 actggcggcg gggcgtcggt gtagacagca tcaaccatgc tgatgctccc catattgacc
     7141 atcgcattac ggccaaattt cgagtgatcg acaaccagca taacgtggcg cgagttctca
     7201 ataatagcgc gtttggtgcg aacttcgtgg taatcgaatt ccagcaacga gccgtcgcta
     7261 tcgatgccgc tgatccccag aatgccgaaa tcaaggcgga actgggagat aaaatcgagc
     7321 gtcgcttcgc ctatgatccc accatcgcgg ctacgtaatt cgccaccggc gagaataata
     7381 cggaaatctt cttttaccat caacgtgtta gcgacgttga gattgttggt gacaatgcgc
     7441 aaattgctgt gattaagcag cgcgtgcgcc accgcttccg gggtggtgcc gatatcgata
     7501 aacagcgtcg agccgttggg gatttgctcc gccactttgc gggcgatgcg ctctttttct
     7561 tcggtctggg tggccttgcg atcgtgccac ggggtgttaa ccgaactgga aggcagcgcc
     7621 gcgccgccat gatggcgcag gatcaggttt tgctctgcca gctcattgag gtcacgacga
     7681 atagtctgcg ggctgacgga gaagtgctcc accagttcct cggtactgac ataaccttgc
     7741 tgtttaacca gttcgataat accgttgtga cgttgtgttt gtttcattta taaatccctg
     7801 gaattatttt cgttttcgcg cattgagcga atcaacaaaa gccatcgcta aacccacggc
     7861 taacccggcg atgtgtgctc cgttcgccat cgacatccca aacaaatcaa accatccggc
     7921 gacaatccag atcagcgcaa agataattaa cccacgttgc aggtaaatgc cactttgcgg
     7981 atcgcgttcg ccacgtagcc agacgtagcc catcagcgca tacaccacgc cagaaagccc
     8041 gccaaaccac ggcccgctga atttttgctg cacatagccg cttaacaggg cgctaatgag
     8101 agtaatgaca attagcttac cgctaccgag gcgtttttcc accgcaccgc cgagatacca
     8161 ccaccagagc aggttaaaga ggatatgcat cagcgagaag tgcattaacg cgtgggtgaa
     8221 gtaacgccag aactcaaatt tcagtgttgg atcgaatggc caggccagcc ataacatcac
     8281 ttcctgatcg ccgagaattt gcatggcaat aaacaccacc acgcaggcga tcatcatcac
     8341 ccaggttacc ggacctgcgc gttcacgcaa ggcagcaaag aaaggataac ggcgataatg
     8401 caggccactg ccggtatggc ccgcctgcca gctggccgcc agataacgcg aatctgccgg
     8461 gttttcgaga aaacgcgcca gctccgcccg tacgcgctcg gcctgggact catccgccag
     8521 ccagacgtcg ctttggttat gttgttgaat cgtgaggata acaccctgcg tcgccatgta
     8581 atcaacaaac gcctgcgcca cgcgggggtt agcaaaagag gtaatcatca acattgttgc
     8641 tgtcgcttat tccacacaaa aggggacagt ataaagcgtt acgcgccgta cgccacctct
     8701 gcgggaaact gtcgctgcca ggcttcaaag ccgccgtcaa tgctatagac cacatcgtag
     8761 ccctgttgca gcagatactg cgccgcccct ttgctgctat tgccgtgata acacatcacc
     8821 atcaccggag tgtcaaagtc gttatcacgc ataaacgcgc ccagtgtgtc gttggttaaa
     8881 tggaaagcct gcaccgcatg ccccattgcg aaactctgtg gatcgcgaat atcgaccagc
     8941 accgcctctt tttcctgcaa cttctggtgc gcgtcggcaa cgttaataca ttcgaactga
     9001 tccatgcgtc tctctttctt tacaaacaag tgggcaaatt taccgcacag tttacgtcga
     9061 agcggcagat aaacgccata atgttataca tatcactcta aaatgttttt tcaatgttac
     9121 ctaaagcgcg attctttgct aatatgttcg ataacgaaca tttatgagct ttaacgaaag
     9181 tgaatgaggg cagcatggaa accaaagatc tgattgtgat aggtggcggc atcaatggtg
     9241 ctggtatcgc ggcagacgcc gctggacgcg gtttatccgt gctgatgctg gaggcgcagg
     9301 atctcgcttg tgcgacctct tccgccagtt caaaactcat tcacggtggc ctgcgctacc
     9361 ttgagcacta tgaattccgc ctggtcagcg aggcgctggc tgaacgtgaa gtgctactga
     9421 aaatggcccc gcatatcgcc ttcccgatgc gttttcgcct gccgcatcgt ccgcatctgc
     9481 gcccggcgtg gatgattcgg attggtctgt ttatgtacga tcatctgggt aaacgcacca
     9541 gcctaccggg atcaaccggt ttgcgttttg gcgcaaattc agtattaaaa ccggaaatta
     9601 agcgcggatt cgaatattcc gactgttggg tagatgacgc ccgtctggtg ctcgccaacg
     9661 cccagatggt ggtacgtaaa ggcggtgaag tgcttacccg gactcgtgcc acttctgctc
     9721 gccgcgaaaa cggcctgtgg attgtggaag cagaagatat cgataccggc aaaaaatata
     9781 gctggcaagc gcgcggcctc gtgaacgcca ccggcccgtg ggtgaagcag ttcttcgacg
     9841 acgggatgca tctaccctca ccttatggca ttcgcctgat caaaggcagc catattgtgg
     9901 tgccgcgtgt gcatacacaa aagcaggcct acattctgca aaacgaagat aaacgtattg
     9961 tcttcgtgat cccgtggatg gacgagtttt ccatcatcgg caccaccgat gtggaataca
    10021 aaggtgatcc aaaagcagta aaaattgaag agagtgaaat caattacctg ctgaaagtgt
    10081 ataacacgca ctttaaaaag cagttaagcc gggacgatat cgtctggacc tactccggcg
    10141 tgcgtccgct gtgtgatgat gagtccgatt cgccgcaggc tatcactcgt gattacaccc
    10201 ttgatatcca tgatgaaaac ggcaaagcgc cgctgctgtc ggtattcggc ggtaagttga
    10261 ccacctaccg caaactggcg gaacatgcgc tggaaaaact gacgccgtat tatcagggca
    10321 ttggcccggc atggacgaaa gagagcgtgc tgccgggtgg tgccattgaa ggcgaccgcg
    10381 acgattactc agctcgtctg cgccgtcgtt acccattcct gaccgaatcg ctggcacgtc
    10441 actacgcgcg cacttacggc agcaacagcg agctgctgct ctgcaatgcg ggggcgatag
    10501 gcgatctcgg ggaagatttc ggtcatgagt tctacgaagc ggaactgaaa tacctggtgg
    10561 atcacgaatg ggtccgccgt gccgacgatg ccctgtggcg tcgcacaaaa caaggcatgt
    10621 ggctgaatgc ggatcaacaa tctcgtgtga gtcagtggct agtggagtat acgcagcaga
    10681 ggttatcgct ggcttcgtga tttaacgtaa ggtggtcagg tcagatttca atctgacctg
    10741 agactgatga taaacaaaaa aactgcctga tgcgctacgc ttatcaggcc tacgtggttt
    10801 atgcaatata ttgaatttgc atgattttgt agacaaaata aggcgttcac gcagcatccg
    10861 gcataaacaa agcgcacttc gtcaacaatc tgacctactt tttgattcaa ggaattaccg
    10921 caaagcccac gcaatcatac ccaacgtaac aaaactcaga cacattaccc actcgcccca
    10981 cttcttcctc tgacttctat ttttgctacg ccactggcaa taatatccca atgcaaaacc
    11041 aagctgggcg cttatcggac cgataaagtc agtccacggc tgagacccaa attgaaaaaa
    11101 ttcaggggta accagacaat ggtcagaaag aaattgcgca tattttccag caaagcaatt
    11161 tgcgctggcg cgacatagaa aatgatattc cacaggagca accagaaata aactggtgca
    11221 aataccaacc aacaatggag tctgcttttc agttcagatt gagctctgca agaaaattgc
    11281 caaccaacgc aacacatggc acagaaaaaa aaagtgcata ttcagccaat aacccaagtt
    11341 ggaattccag aagtgaaata cgcctgaaca tgagcgggat agagaacagt atttctccca
    11401 taaaactcag cgtgataaca caagcagcca gcgcaaccac cgtcggccac accaacaacg
    11461 gcacaataac aaaaccaaga attaacggag gaatataaag tctgatttta ttcatcttta
    11521 ttgtcctgat gattcggatg atgcgtcata aatcggcaca tagcccacat cctctaccaa
    11581 ttgctgtccc tgcgggctta aaaaccagtc gacgaatttc tgcgtttcca gcgtagggtt
    11641 ttcgtgcgtc accatataag catgaattat gtacggataa gtgccgttac ggatattttc
    11701 ctctgtaggt gcaacgccat ttatagccag cagtttgatc tgtttgcgca ttttgagcat
    11761 attgtcattc atacgtgtga cgtaatagtg gaaggtgtat ccaatagaag atggtgtatt
    11821 ttggtagtcg gcgacttcgg taattaaacc gcccataaga tcgataacag tggatttttt
    11881 ggctggcagc attggagtat cttgcataac cagtcccttc ataaccgttt ggcttccaga
    11941 atcttctggc cgctgccaga tttttatacc ttcgtcaccg ccgcccactt tattccagcg
    12001 actcacttta ccgctaaaaa tgtctttgac ctgctgttcg ctaagtgaat taaccgggtt
    12061 tttgatatca acgataaaga caaaggcttc gcgggcgaaa gcggtatatt tgagtttaac
    12121 acctgcttct tctgcacgtt ttttctgtcc ttccgacggt tgtgcaacaa aaataaccgt
    12181 cgcggtaccc ttaatcagac tatcgtaggc ttcagctgtt cgggtttgtt gcaaaccata
    12241 cgcctgccag gccagcgagg ttcgatcctt atcttttggc tcgggtacta attcataata
    12301 cgcggaggca tacagcgggt ataaggctgt agcgccgtac actctcggcc atcttgacca
    12361 atcatcgttg taacgtaatt caggttgccc ccgcagagac gtcagtccat ggctgtcttc
    12421 ttccctgacg ctgttatagt acccttcccg gtcgacacat cccgccagta gcgccgtacc
    12481 taatagtaaa acacaggtga atttattcat tgcgctgatt cctttctctc gagcgttatt
    12541 tcattgttgg atacagcggc acatagccca catcttccac cagactctgc ccctgcggcg
    12601 ttaaaaacca ttcaaccagt ttttgtgttt ctgacgtcat attgtcccgc gttaccataa
    12661 atgcatcgac gacataggga tatttgccat tgcgaatatt ttctgcagtc ggtgcaatac
    12721 cgttaatcgc tagcagtttt atatttttat cggcattcat ttgtgttgcg taatagcgga
    12781 aggtgtagcc tattgcgttg tttgtattac ggtattcggc aacgacttta atcattccct
    12841 ccatcatgct tgccacttcg gtttcctgcg gcgagatcat gcggacattt ttcatgacct
    12901 gcgattgcat cactgtctgg ctgccagagt cttccgggcg ttgccaggtc tggatctcct
    12961 gatcgttacc gccaaccgtg cgccagttgg taattgcgcc actaaaaatg tcacgcactt
    13021 gttgttcagt cagggaatta accggattat ccgcattgac gatgaaaaca aacgcttcac
    13081 gggcaaatgg cgtgtacagc aaagtgacgc ccgatttctc agcgcgtttt ttttgcccat
    13141 cggaaggttg cgccacgaaa ataatatcgg catcaccgtt aacgattttg ttatatgctt
    13201 cttgcgtacg agagttatcc agataatccc aaacgtgaaa atcctctggt attacactta
    13261 atgcataaaa tgcagaggca taaatggggt acgccgccgt agcgccatcg agtcgcggcc
    13321 agttttgcgt aaattgaatt tgtggtttac cacgcagtgg cgtcagttga ttattaagtt
    13381 tatcgggtcg ccaggcccag gtatcaatat tttcacgcac accgacgccg ggattaagct
    13441 gtttatataa ataagcctga taaccagcga caatacccag taagagaaga atgaacagaa
    13501 gtaaattacg gcaacgtaga ccggaggtgc cggttaaagg gtgtctggtc ggccagccgt
    13561 atcccagtga aaaactgata tgcgcgccaa cgggaataat gactatccac cagtactcac
    13621 caccgacgaa tgagactgta aaaacgccca accagggaag cataaggttt gccaaccaac
    13681 cggctcccat ctggcgaaat tgataatctg ccagatgtag cgtgattatc cagccaacca
    13741 gggttaataa agcgggaact aaaaaaagac attgtcgcca ggcggctatt tctctgtttt
    13801 ctggtgcgct atcgcccgcc cacactcgcc cagagaaaaa tagcagtaat gcgcccagcg
    13861 tcagcataaa gacgttgagt agaagataaa ttgttggtgt aaagagtaaa ttgcaaaact
    13921 caagaatagc ggcaagcccg gcccccagca tggcaacaac cgctgccaaa aattgcgcca
    13981 gtatggggat gccaaaaaaa gtcattacca gcgaggtcaa aatccatttt ccgttttgca
    14041 ttattctttc cattcttgct gaatggcgaa attatactcc ccgataaccc tcgccccctc
    14101 tggacacttt tcctaaataa aggcgaaaaa aaacgggacc ttttggcccc gttctgtttt
    14161 ttagcgaact tacaatctca ccggatcaat atgccagata tgatcggcgt actctttgat
    14221 cgtacggtca gaagagaagt agcccatatt ggcaatgttc agcatcgctt ttgcggtcca
    14281 ctcttcctga cgctcgtaga gttcgtcgac tttatcctga caatcgacat agctgcgata
    14341 atccgccagt acctggtagt gatcgccgaa gttgatcagc gaatcgacca gatcgcgata
    14401 gcgacccgga tcttccggac tgaatacacc gctgccgatt tgcgtcagca cctgatgcag
    14461 ctcctcatct ttctcgtagt attcacgcgg tttgtagccc tgacgacgca gttcttccac
    14521 ttcttccgct gtgttaccaa agataaagat attgtcagca ccgacatgat ccagcatctc
    14581 gacattcgca ccgtccagcg taccgatagt cagcgcgccg ttaagcgcaa acttcatgtt
    14641 actggtgccg gaagcttccg tccccgccag cgaaatctgt tcagatagat cggccgccgg
    14701 aatgatcaac tgcgccaggc taacactgta gttcgggatg aacacgactt tcagcttgtc
    14761 gccaatctgc ggatcgttgt tgatcacttt cgctacgtca ttgatcaaat gaataatgtg
    14821 cttcgccatg taataggccg aagccgcctt accgccaaaa atattcacgc gcggtaccca
    14881 cttcgcatcc gggtcggcct tgatgcggtt atagcgggtg atcacgtgca acacattcat
    14941 caattgacgt ttgtattcgt gaatacgttt aatttgcaca tcgaacagcg ctttcggatt
    15001 taccaccaca ttcagctgct gggcgatata ctccgccaga cgctttttgt tctccagctt
    15061 cgcctgatgc accgcgtgat taaccattgg gaaatcacag tgttgttgca gctcattaag
    15121 caggctaagg tcggtgcgcc agttacggcc caggtgttcg tccagcacag ctgaaagcga
    15181 tgggttcgct accgccagcc agcgacgcgg ggtcacaccg ttggtaacgt tggtgaaacg
    15241 acccgggaag attttcgcaa agtcggcaaa caacgattgc accatcaggt tagagtgcag
    15301 ttccgataca ccgttaactt tgtggctcac cacaaccgcc agccaggcca tacgcacacg
    15361 acgaccgttg gattcatcaa tgatcgacgc ccgtcccagc agatcggtat cgttcggata
    15421 ctgttcctgc aaggttttaa ggaaatagtc gttgatttca aagatgatct gcaggtgacg
    15481 cggcagaatt ttacccagca tatcgactgg ccaggtttcc agcgcctcgc tcattagcgt
    15541 gtggttggtg taggagaaga cctgacaaca cacttcaaac gcgtcgtccc agctaaattg
    15601 gtgctcatcg atcagcagac gcatcatctc aggaatcgac agcaccggat gggtatcgtt
    15661 gaggtgaatc gcaattttat ccgccaggtt atcgtaggtt ttatgcaact gataatggcg
    15721 gcttaaaatg tcctgaatgg tcgaggaaac caggaagtat tcctgacgca gtcgcagctc
    15781 acgcccggag taggtggagt cgtccggata cagtacgcga gatacgttct cggagtggtt
    15841 tttatcttcc actgccgcga agtagtcgcc ctggttgaat ttaccgaggt taatttcgct
    15901 actggcttgg gcactccaca aacgcaacgt gttggtcgcg tcggtgtcgt aacctgggat
    15961 tatctgatcg taagctacgc ccagaatctc ttcggtttca atccagcgcg tttttttacc
    16021 ttcctgctga atgcgaccgc caaaacggac tttatagcgc gtgttgtggc gtttgaattc
    16081 ccacgggtta ccgtattcca gccagtagtc aggcgactct ttctggctac cgttaacgat
    16141 gttctgcttg aacataccgt agtcatagcg gatgccgtaa ccgcgccccg gcaaccctaa
    16201 cgtcgccaga gaatcaagga agcaagccgc cagacgtccc aggccaccgt taccgaggcc
    16261 cgggtcattt tcttcatcaa tcagctcttc gagatttaac cccatcgctt ccagtgcgcc
    16321 ctgtacatct tcgtaaattc ctagcgacaa catggcgttg gaaagcgtac ggccaatcaa
    16381 aaactccatc gacaggtagt aaacctgacg agtttcttgc gacaactggg cacggtttga
    16441 acgtaaccag cgctccacga gacgatcgcg cacagcaaat aacgttgcgt tcagccattc
    16501 atgtttattg gcgacgaccg ggtcctttcc aatcgtaaac atcagcttgt aagcgataga
    16561 gtgcttaaga gcttctacgc taagcgtggg cgatgaatat gtaaacggag cattcatata
    16621 ggcgtttcct gaaaactatt tcaagcgata gtaaagctca cggtacgact tcgccgcgac
    16681 ctgccagcta aaatccattg ccatagcctg acgttgcaca aaccgccaca gtgaaggacg
    16741 agaccacagt acaaaagcac gtcgaatagc ccgtaacagc gaccaggcat tactatcttc
    16801 aaagacaaac ccactggcga cgccatctgc aaggttctca agagaacaat cagaaaccgt
    16861 atcagcaagc ccaccggtgc gccgcactaa cggcagcgta ccgtacttca atccataaag
    16921 ttgcgttaag ccgcacggtt caaaacgact gggcaccaga atgacgtccg cgccgcccat
    16981 aatgcgatgc gaaaatgctt cgtgatagcc aatctgaacg cccacctggc cggggtattc
    17041 cgctgccgcc gcaaggaaac cttcctgcag caccggatcg cccgcgccga gtagcgccag
    17101 ttgcccgccc tgctccagaa gacccggtaa ggcctccagc accaggtcga gacctttctg
    17161 gctggtcaga cggctcacca ctgcaaaaag cggcacttta tcgtcaacct taagccccat
    17221 tgcgatttgt aactggcgct tattttccgc tttatcttcc aacgtatcgc gggtgtaacg
    17281 cgaggccaac agtaagtccg tctctggact ccagattttc tcgtccacgc cgttcagtac
    17341 gccggaaaga cgcccttcac ggtgacgctg ttgcaacaga ccttccatac cgtaggcaaa
    17401 ctgcggttcg gtgatctcgc gagcgtaggt tgggctgact gccgtaatgt gatcggcata
    17461 gtacagaccg gccttcagga aagagatttg tccgttgaat tccagcccat gaatattaaa
    17521 gaacgaccat ggcaattgga tgtcattcat gtgatgtgca taaaacatgc cttgataggc
    17581 caggttgtgc acagtaaaca ccgacttcgc cggacgcccg cgcgccgcca gatacgcagg
    17641 cgcaaggcct gcatgccagt cgtgcgcatg caccacatca ggacgccaga atgggtcaag
    17701 cccgctggcc atttctgccc caacccaccc cagcagcgca aaacgcaaca cgttgtcggt
    17761 ataggcaaat aagttggtat cgtgatacgg gcttcccgga cgatcataga gatgcggcgc
    17821 gtcaatcagg taaatgccaa ccccgttgta atgaccgaac aacagcgtga tatgtccggc
    17881 gaaggtatca cgacgggata ctacctgcgc atcggtcacg ccacggcgaa tatcgggaaa
    17941 tgcaggcaac agtacgcgag cgtcaacgcc gtctgcgatt tgtgctgcgg gtaatgcccc
    18001 aataacatca gccagaccgc cggttttaag cagcgggaac atctctgaac atacatgtaa
    18061 aacctgcatt atcgctcctg tttatgccct aacttccgta gcatttcgcg cgttaccagc
    18121 acgatgcctt cttctgaacg atagaaacga cgtgcatctt cctctgcgtt ttcaccaatc
    18181 accatgcctt ccggaataac acaagcacga tcgatgacgc agcggcgcag acggcacgag
    18241 cgacctaccc atacttccgg taacaatacg gcggaatcaa tgttgcagaa tgaattcacg
    18301 cgaacgcgcg agaacagaac ggactgcacc accaccgaac cggagatcac acaaccgccg
    18361 gataccagtg agttaagggt catcccgtgg ctaccggagc gatcctgcac gaatttcgct
    18421 ggcggtaatg attcattgta ggtgcgaatt ggccaattgc gatcgtacat atccagttcc
    18481 ggcaccacag aggccagatc gaggttcgct ttccagtaag cttccagcgt acccacatcg
    18541 cgccagtacg gctcggcatc cgggtcggat tgtacgcaag agagcgggaa tgggtgtgca
    18601 taggccagac cggcttcggt gatcttggga atcaaatctt tgccaaagtc gtggctggag
    18661 ttctcatcgc gatcgtcttc ttccagcagt tcatacagat agtcggcgtc aaagacgtag
    18721 atacccatac tcgccagaga tttgctcgga tcgttcggca ttgacggcgg gttagctggt
    18781 ttttcaacga attcgataat tttatcgttc tcatcaaccg ccataacgcc aaatgcggag
    18841 gcttcttcaa tcggtactgg catacaagca acggtgcaac gtgcgccttt ttcgacgtgg
    18901 tcgataagca tacgcgagta gtcttgcttg tagatatggt cgcccgccag gatcaccacg
    18961 tattccgctt tataacggcg gataatgtcg aggttttggg taactgcatc tgcggtgccg
    19021 cgataccagt tttccccttt cattctctgc tgtgctggca gcagatcgac aaactcgttc
    19081 atttcttcat tgaagaatga ccagccgcgc tgaatgtgct gcaccagagt gtgggactgg
    19141 tactgggtga tcacgcccat acgacggatc ccggagttga tgcagttaga cagcgcaaag
    19201 tcgataatgc ggaacttacc gccgaagtgt acggccggtt ttgctcgctt attggttaaa
    19261 tccttcaggc gggtaccacg tcctcccgcc agtatcaggg caacagattt caatggcagc
    19321 tggcgcgcca acattaagtg atcgttcttc tctaaactaa ccatgactaa ctcctttttt
    19381 atcatctctg gaacacacac aatccgtgtg caggtccctg ccagacagcc gtaatcactg
    19441 ggttatcctc tccagcgaat gggggaatgg cgtgccactc cccagcaggt aaaacaatct
    19501 ctgttacctc aagcgtggcg ttaattgcga tcaaaaagcg atccgagagc agaatttgca
    19561 gctgtttcgg cccgttttgc cactcatccg tgcttaaagg ttgagcatat cgatttagcc
    19621 aacggacatt gccgtcgcct tcttcccacc agcgattctc caccaaagcg ggaatgcgct
    19681 tgcgcagatg gattaacgcg gcggtaaatg cggttaaacc actgcttgcc tgcgaccagt
    19741 ccaaccaggt taattggtta tcctgacagt aggcattgtt attgccatgc tggctgtgac
    19801 catgttcgtc accggccagt aacatcggcg taccctggga gagcaacaac gttgttaaca
    19861 gggcgtgaat gctgtcgcga cgccgttcaa ccaggtcaag agtaccgcct aacccttctt
    19921 taccatgatt gttactataa ttgttgttgg tcccgtcgcg attttcttct ccgtttgctt
    19981 cattatgttt atggttgaag caaacgcagt cgcgaagcgt aaaaccgtca tgcgcggtga
    20041 cgagattaat cgcggcactc ggcagacgac cattacgttt aaaaacatcg ctggaggcag
    20101 caaaacgccc ggcaaacgcc cccagaggca aatcataatg aagccagaaa cgacgggcag
    20161 catcgcggaa atgatcgttc cactcggcaa acagcggcgg gaaatttccc acctgataac
    20221 caccaggagc aatatcccac ggttcagcaa ttaacttcac ctgcgagagc accgggcagt
    20281 tctggatagc ggtaaacaac ggcgcatcct gacggaactc tggcgtacgg cccatgactg
    20341 ccgccagatc aaagcggaaa ccatcgacgt ggcaggtttc tacccaataa cgcaggcaag
    20401 cgctggcata atccactacc gccggatgac tcaaattgag cgtgttgcca caaccggtcc
    20461 agttgtgata atcgccgtct tctcttatcc aataatagct acggttgtcg atcccacgca
    20521 gagagaataa cggaccgtcg aggtccagtt ccgcactatg gttgagcacg atatcaagaa
    20581 tgacttcgat acccgcttta tgcagtgctt tgattgcatc gcgaaactca tccagcgccg
    20641 tttctgggga gcaggcatac gccggatgca gcgcaaacat cgccaccggg ttataacccc
    20701 agtagttact tagacccata cgttgcaggc gtggttcgct ggcaaactgc gccaccggca
    20761 gcagttccag cgcggtaatg cccaactgtt tcaaatagtt gatcatcacc ggatgcccca
    20821 gggctttata agtgccacgg atctcgaccg ggatctccgg atgcaggtac gttaatcctt
    20881 tgacatgggc ttcataaatg atggtgctgc cccacggcgt gcgcggcggg gcatcatctt
    20941 cccagtcata gtgatcaacc accactacgc atttcggcgc aatggcggcg ttgtcgcgat
    21001 agtcaggttc attatgaccg gcgtgcagca gcgggttatc tttaaattcc ccgtcaattt
    21061 gccgcgcgca aggatcaatc aacaacttcg ccgggttaaa gcgatgcccc tcggcgggtt
    21121 gccaggggcc atgaacgcga taaccataac gcaaacccgg acgcgcatcc ggcagataac
    21181 cgtgccaaat gtcgccactg tgccctggca agtcatagcg atgttcctgg ccattggcgt
    21241 caaagataca cagttctacc cgctcggcat gagcggagaa aagtgtgaag ttgacgccct
    21301 gaccgtcgta atgcgcgccg aggggtgcgg gtttgccaat ggcgagttgt gtcattctgc
    21361 ctcccgaacc agccaaatag tggccagcgg tggtagtgtc aggcttaatg aatgctgacg
    21421 accgtggctg gcaatctcat cgctgtgtac cgtgccgcca ttgccgacat tactgccgtg
    21481 atagtgcatg gaatcggtat taaggatttc acgccatttg cccggctggt taatgccgaa
    21541 gcgataatca tgacgcggca ccggagtaaa gttactggca acgatgattt cgttaccctc
    21601 tttatcgcga cgcacaaaga tcagcaccga gcgttctttg tcatccacca ccagccattc
    21661 aaagccgtac gggtcaaaat ccagttcatg cattgcttta tggtggcggt aggtgaggtt
    21721 cagatcgcgc accagacgct ggacaccgtg gtgccagtta tcgccgcctt ccaacagatg
    21781 ccagtcgagg ctggcgtcat ggttccactc gcggccctgg gcaaattcgt tccccatgaa
    21841 cagcagtttc ttgcccggga atgcccacat ccagccgtag taggcgcgca ggttagcgaa
    21901 tttctgccat gcgtcgcccg gcatccggtc gagaatcgat tttttaccgt ggaccacttc
    21961 atcatgcgac aacggcagga cgaagttttc agtgtagttg tagagcatcc cgaaggtcag
    22021 tttatcgtga tgatactgac gataaaccgg gtcgagcttc atgtagtcca gggtgtcatg
    22081 catccatccc aggttccact tgtaccagaa gcccagaccg cccatgtcct gcggacgaga
    22141 aacgccaggg aaatcggtag actcctccgc cattgtcacc gcaccggaaa cctgctcacc
    22201 aagaatacgg ttggtattac gcaagaattc aatcgcttca agattctcgc gcccgccaaa
    22261 ttcgttcggg atccactccc cctctttacg gctgtagtcg cgataaatca ttgacgccac
    22321 cgcatcgacg cgcagcgcat caataccaaa acgttcaatc cagtaaagcg cgttaccgac
    22381 aaggaagtta ctgacttcac ggcgaccata gttgtagatc agcgtattcc agtcctgatg
    22441 atagccttcg cgcggatcgc tgtgttcata caagttcgtg ccatcaaatt cggcaagcgc
    22501 aaagtcatcg gtcgggaagt ggcctggcac ccagtcgaga atcacgttca gaccagctgc
    22561 gtgtgcggca tcaatgaaat aacggaagtc gtcgcgagta ccaaaacggc gggtcggtgc
    22621 atacaggccg gttggctgat aaccccaact gccatcgaag ggatgctcgt taatgggcag
    22681 cagttcgagg tgggtaaagc ccatccattt agcataaggc accagttgat cggccagctc
    22741 gcggtagctc aaccagaaat tgttgtcggt gtggcgacgc caggagccca ggtgaacttc
    22801 ataaatagag attggcgcat caaactgatt cgcttttttg cgctcttcag tctgcacaac
    22861 cttttccggc agaccgcaaa taagagacgc ggtttccggg cgcatttgcg cttcgaaggc
    22921 ataagggtcg gacttcagac gcaagttgcc attggcatca atcatctcat atttatagag
    22981 ctgaccgtta tgcgccccag ggataaacag ttcccagatg ccgctctctt tacgcaggcg
    23041 catcggatga cggcgaccgt cccagtagtt gaattgccca accaccgaga cccgacgggc
    23101 gtttggagcc cagacagaaa aacgcgtacc tgtgacgcca tccatagtat ctgcatgcgc
    23161 gcctaaggtt tcatacgggc gcaggtgagt accttcagat aatagccagg catccatttc
    23221 ctggattagc ggaccaaaac ggtaaggatc atctatcagg ttttgctgac catgccagac
    23281 aacagccaac tgatagcgga aaaaattctt acgtcgcgga atgacaccgc taaagaatcc
    23341 acgggagtcg agacactcca gttttgcgag tttgcgcccg gttttcggtt caatcaccca
    23401 cacatcggta gcgtcgggta aaagggcacg gacttccagt cccgcggtgg ttttatgcat
    23461 ccccagtacg gaaaaaggat ccgcaaaatg gcctgcaatt agcgcgttaa tcacgtctct
    23521 atcgatacga tcggacatgc ttgtcttcct gttttattgt gtcaccccat ccaactggat
    23581 ttttgacttc tggttgtgac atttttttga cctgaacggc gcagcactct gtgcatcctc
    23641 tctgcgtcgt cctcacttca ggtaaggctg tgaatactca tgtattcagc cacccttaaa
    23701 gaatagccaa tgctctattt aactcccggt aaatcatgaa acatctgcgc ttactcctgt
    23761 attacgcact agcaggggcg gcatcgcgcc ccagatttaa tgaataaaga ttacgccagt
    23821 tgacgaagca tccgacgcag cggctccgcg gccccccaca gcagctggtc gcccacggta
    23881 aaggctgaca ggaactctgg tcccatattc agcttacgca ggcggcctac cggcgtggtc
    23941 agcgtgccgg taacggcagc tggggttagc tcacgcatag tgatttcccg atcgttcgga
    24001 acgactttcg cccacggatt gtgcgcagcc agcagttctt ccacggtcgg aatagacaca
    24061 tcttttttca atttaatagt gaatgcctgg ctgtggcagc gcaatgcccc gacacgcaca
    24121 cataaaccat ctaccggaat tacggaagat gtgttgagga tcttgttggt ttccgcctgc
    24181 cctttccact cttcgcggct ctgaccgtta tcaagctgtt tgtcgatcca cggaatcagg
    24241 ctacccgcca gcggcacgcc aaagttatcc accggcagct caccgctacg ggttaaggtt
    24301 gtgactttgc gttcgatatc gagaatagca gaggacgggg tcgcgagttc atctgccaca
    24361 tggctataca gatggcccat ctgggttaat aactcacgca tatgtcgcgc accaccgccg
    24421 gaagcggcct ggtaggttgc aacggacacc caatcaacaa gatcattggc gaataaacca
    24481 cccaacgaca tcaacatcag gcttacggta cagttaccgc caacaaaagt cctgatgcca
    24541 ttatttaatc cgtcggtaat gacgtcctga ttgacggggt caagaatgat gatggcgtca
    24601 tctttcatgc gcagagacga tgctgcgtca atccagtaac cttgccatcc gctttcacga
    24661 agctttggat agatttcgtt ggtataatcg ccgccctgac aggtcacaat gatatcgagg
    24721 gcctttagcg cctccagatc aaaggcatcc tgaagtgtgc cagtggttcc gccaaaagac
    24781 ggcgcagcct gaccaagctg agaagtagaa aagaagacag ggcgaatggc gtcgaagtcg
    24841 cgctcttcaa ccatgcgttg catgagaacg gagccgacca taccgcgcca gccgataaaa
    24901 ccaacatttt tcataagcgt tttttcctgc aaagatgtgt gctgtataaa tgtgccggtc
    24961 tcctcttggc acatctttca ccatacaaaa agcagccaaa gtcgcaagtg aaattaatca
    25021 atgatagcga agccatcagt aatgcgactt atcctgcttt gttagcacgc agaaagtccg
    25081 cggcaattat cagggaattt gagttatgaa tgaaatcatt tctgcagcag ttttattgat
    25141 cctgattatg gatccgctcg gaaacctacc tattttcatg tccgtactga aacatactga
    25201 accgaaaaga cggcgggcaa tcatggtgcg agagttgctt attgctctcc tggtgatgct
    25261 ggtgttcctg tttgcgggtg agaaaattct ggcatttctt agcctgcggg cagaaaccgt
    25321 ctccatttct ggcggcatca ttctgtttct gatcgccatt aagatgattt tccccagtgc
    25381 ttcaggaaat agcagcgggc ttccggcagg tgaagagccg tttatcgtgc cgttggcaat
    25441 tccgttagtc gccgggccga ctattctcgc cacgctgatg ttgttgtctc atcagtaccc
    25501 gaatcagatg ggacatttgg taattgctct gctgctggcc tggggtggca cctttgtcat
    25561 cctgctacag tcttcgctat ttttacgtct gctgggcgag aaaggggtga acgcacttga
    25621 acgcctgatg ggattgattc tggtgatgat ggcaacccag atgttcctcg acggcattcg
    25681 aatgtggatg aaggggtaag gttcaaacag gtgctgcaca caaccagagg cggcacctgt
    25741 tttattacct gaactgataa tgaccttcgc ccaatcggat aacccttccc tcttcttctg
    25801 cgcggttcag ccacatacta atttgttcga tggttaaacc agtacaagac gccagccttt
    25861 caatacttat cgattccttt gcaaggatcg ccagttccgc cacaaaaagt tgataaaaat
    25921 cgacaggggt atgcgagggc atcactgcgt aatttgcgtc atcttcgtgc agcgaaaaca
    25981 attctggttg agcgttacgg agtgctctgt catacatcag cccaggaacg tctggcagtc
    26041 tgatcgtcac attctcagcc tgttcagcac tccatgacgc cccattttct actaatcgtg
    26101 aattggctga ttgcacatcc tgatttggtt taaccagaac aggcagccac tgatgtttta
    26161 acgcctccat cgctccggtt atcgttccac cggtcattcc cgcacgtaca accattgcgc
    26221 tttcagcaag gcaataaata tatttattcc gcgccatcgc attgccgacg gtgaaacgta
    26281 cctctgggta aaacggtgaa atcaacacca ggttgcctgc tataagccct tcacgccatt
    26341 tcactaacgt gctcgttttt aacaagctat cagctaatac gccaacggca gttcctccgg
    26401 cctccagcgc cgacgctatt gcacattcat caatacctcg cgcaccacca gagataacgc
    26461 aaatcccctg ttgggccagt ttcgctgcca gttgttgggt atagcgcaaa tcggcagtcg
    26521 gagcatctcg cgagccaaca atcgccatac cttccgcttg cagtaatgct ttattcccgc
    26581 agccaaataa aacgggaggt gcatccgttc gcaatcggtt tttcaagcga acgggataat
    26641 cggcatctcc gcgggttaag atccataaac cggcgcgttg ccacttatct accgccaacg
    26701 ccagactatg accacgcgcc agcaagccaa gaatacgctc ctgagaaata cgcggatcgc
    26761 tccagcctgt aagtttctct tgcggttgcg gcaccagaag atcggcggga ctgatacgtt
    26821 ggtgcttcag ccataatgca aacttccccc attcactatt actaagaggt ttatactcac
    26881 tcgccgccgc gcgagaaaaa tcgctggtta gcaggagagt cgcttgtgca ttggctgaaa
    26941 gattcatcaa ttttttaccg aggtagaagc aagggcaaga ggataaaccg tcgggcaacc
    27001 tgcctggcgt aacagtgctg tcagaactgt cagcgtccat gcagaatcca cgatatcgtc
    27061 aaccagcaac gccggacctg gcatcaaagg aggggtaatc acaaacgccc cgtcgagatt
    27121 ttgacactgg tgaaaacggt tttgctgcat tttctgcggt ggattgtcca cgactttttc
    27181 aatggcatca ataaaaggta agccaagttt cgccgccagt cgccgggcaa aatcaggaac
    27241 caggtcgagg tgccttaatg aaggaacgca gcatacccag gttggttcag gatgcggatt
    27301 ccagcgttgg cgaaccattt ccgcacatgc ttctaccagc tcatcactta agcgccccgt
    27361 ttttttctcc tgtgctacct gctttcccca cccggaatct ccccaacgag aaaggattcg
    27421 ccccgtagat ccttgtaaac ccgcaggcaa gttcccttta aatccgtatt gcgtaaaagc
    27481 cccagctgcc acctgtttat tgagatttaa cggcaggtca gcgtgtttaa taaataaatt
    27541 tgccgcatgg agtaagccgc tatcaatgtc gggcgacaat aacggatgct gaagacaact
    27601 gctacattta ccacaacgtt cggcacttgg ctcatcgagt gcacggcgca gaaattgcat
    27661 tttgcactcc ttggtcgtga tatagctttc aacgtctgcc agttcacttt ttctctgatt
    27721 catcaaatgg ttaattcgtt catgatcagg agaaaaactg acgatagttc tgcgccataa
    27781 tttctcggta tacaccacag gcgatggatt ttccgctacc aataatttca gtgctttttc
    27841 tatttgccca taacgaagat tcgtccgttg ttcaatgcct cgtagggtaa gaccgtcatt
    27901 ctcgctaagt acgttgagta tttcatgaat ttgcgcctcc gcaggaaagg cgctttcacg
    27961 gaagaattga tgaatagcgc ggtcttcacc accacaaagc aatatgccaa ctgcggaatc
    28021 tatagcacgc ccggcacgcc ccacctgttg gtagtaaccg acaatagacc ccggcatctg
    28081 ataatgaatg acgaaaccta aatcaggttt atcaaacccc atacctaatg ccgtcgtcgc
    28141 gaccagcact ttgattttat ttgccagcag tgcctgctcc agatattccc tggcagtatt
    28201 agaatcttcc gcgccttcac aagtcacgcc gctgtagtaa gcaaatgcac tgataccatt
    28261 tttccttaac cactcggcga caagttcagc atcgcgagtc gtcagggtat aaacaattcc
    28321 cgatttggaa aactgaggga taaccgttgc taaccatgcc agacgggatg actgttctcc
    28381 taagactaag gcatcaagcg ccaaactttc gcgagccagc gttccacgct gaatcacaat
    28441 gtcacccaat tgctgacgga tatcctcaac aacacggtta ttcgctgtcg cggttgtacc
    28501 cagaacaggg gtattcgcag gcagttggcg caaaatatct aatatacgtc ggtaatccgg
    28561 gcgaaaatcg tggccccaat cagagatgca gtgcgcctcg tcgaccacaa gcaagccaat
    28621 acgatcggct ataggatata aaaccgtttc gataaagtcc tggtttgcca aacgttccgg
    28681 ggaaaccagc aagcaatcga cctcaccttg cagcaactta tcgctaatgc gctgccattc
    28741 ttccctgttt gtagaattca acgtttcagc cgtaatacct aaccgttcag cggcggcaac
    28801 ctggttacgc atcagggcaa gcaaaggaga gatgataatt gtcgggccag cgccgcgatc
    28861 gcggaagatt ttactggcga tgaagtacac ggcacttttc ccccagccgg tgcgctgtac
    28921 aaccagcagt tttcgccgct gattaaccac tgcatctatg gcttcccatt gcccttccct
    28981 gaaagtagcg gtggtatttc ctaacatccg ctgtaaaagc aattcagctc cgtgcttttc
    29041 catctttatc tcctgcgatc tttaggcagt gattagaacg gattattagc aggtaaattt
    29101 gcgagagaga tcaaaaagat gaagagagga gataagagaa atgaattact gcgagcaagc
    29161 tcgcagtaat ttgtcgcagt aattaactca acagctggaa cgcaatcatc ccaacgatgg
    29221 caccaacagt gccgagtatg gtttccatca tggtccaggt tttcagcgtt tcggcttcgg
    29281 tcgcgccggt aaatttaccg aacaaccaga aaccggcgtc gttaacgtgg ctgacaacaa
    29341 tcgaaccacc ggcgatacaa atcgacagcg ccgccatttg cgcaccggag tagttcagtt
    29401 gttcaataac cggcatcacc agtcccaccg ccgttaaaca ggcaacggtg gcagaaccct
    29461 gaatgatgcg cactgcagct gccagcacga agcaggtgat ggcaatcggc aggcccatgc
    29521 cggttaacgc ttcgcccagt gccggaccta cgccagagtc aaccagcacc tgtttgaaca
    29581 cgccgcctgc accaatcacc agcagaatga tccccgccgg ttgcagcgcg tgaccgcaaa
    29641 tctccatcac tttgtctttt ggcatgccct gacgcattgc caggccataa atcgccacca
    29701 gacaagcaac cagaatcgcg gtaaacggat gaccaataaa ctcgaaccat tcgtaagcgg
    29761 tagagccttc tggcacaaaa cgcgcggcaa tggttttcag ccctaccagc accagcggca
    29821 acaggatcag cgacaggctg aatccgaaag atggcatttt gccttcgccg agatgtggtt
    29881 cgctgatgtc gtcaggaata tgcagctcca cgtagcggct gatgaaatta ccccacagcg
    29941 gcccggcaat aatcattccc ggaattgccg cacacaggcc aatcaggatc atccagccaa
    30001 agtcggcatt catctgcgat gccagcagca ttggcgctgg tccaggcacc aggaacgcag
    30061 ccgcggccgc cacgcctgcg aataatggga ttaccagctt caccaggttc gtaccggtgt
    30121 ggcgcgccat tgagaaagca acgctaatca gcagaacaat cgccacttca aagaacagcg
    30181 gtagcgcaca gaccagcccc gcaaggccga tggcataatg cgcgcggctg tgaccgaagg
    30241 atttgagcat tttgacggca atctgatcga ctgcgccggt ttcatgtaag atcttgccaa
    30301 acatagctcc cagggcgaca accaccgcca ggaagccgag ggtgcctccc atcccttttt
    30361 ccatcgtcgc tgcgatttta tcgagcggca taccggaaaa aaggccagcc cccatggaca
    30421 ccaccatcaa agccaggaaa gcgtgcatac gcgccttcat gactaaaaac agcagcagta
    30481 aaacagaccc tactgctgtt aaaacaagcg ttaatgtagt cactacttat ttgccttttt
    30541 taataacctc aatggtgctt gccacaacac cttccagcgg ttgatcgata tccaccacca
    30601 gtacatcggt ttcgtccgca cccggctcct gcagcgtttc aaactgcgtc accaacattt
    30661 gggttttaaa gaaatggcct ttgcgcgctt tcaggcggct ttcaatcaca tcaaaatcgc
    30721 ctttcaggta gatgaacgag agattcggat taccttcacg cagcaagtcg cgatagtgtt
    30781 ttttcagtgc agaacagacg atcagcgaca ctttattggt gcgctgcata gcaaacgcgg
    30841 cgtcgttcag cgcctgcaac cacggtttgc gatcgtcgtc attcagcggt tcaccagacg
    30901 ccattttttc gatattgcgc cttggatgga ggaaatcgcc atcaagaaac gcggcatgaa
    30961 gttgatgcgc cacttcactg gcgaccgcag atttgccgct gcccgatacg cccatcaaga
    31021 cgtaaatgtg gtgatcatgg ttagtcgtgc tcaaagtggt gcccccacaa tacaagaaat
    31081 aaaaatgtta cgggtaactg ttatcggtaa cattgtccgg ccggacaata tcagaagcaa
    31141 tatccatccg tgccttaagt gtataagtgt gagctacttc aaatttgtgg gcttaaatag
    31201 atccgcccgg tgacaaggtg aaacctaaat ctaacatttt cggtgtcaca gattcgccac
    31261 gaatacgcgc cagcaggcgt tcagcgccaa tactgcccat ccgctcacgc ggcgtcagta
    31321 cgctcgccaa ccgtggttcc atcacctgac caatgtcatg accatggaaa ccggcaatcg
    31381 ccatatcgtc aggaactttc aaccccagac gctggcattc aaacgccgcg ccgaccgcca
    31441 ggtcatcatt agtacagaac acgccatcca gctgcggata ttcccgcctc gcctggcgaa
    31501 tcagttcaat accggaagag taagaagaag attgctcaac catcacgcta tacggcacca
    31561 ggcctgcatc cagcatcgcc tgttcgtatc ccttctgttt gatgatagta cgttcgtcga
    31621 gacgtgcgcc gagataggca atgtggcgat gcccgcgagc aataatggcg gtggtcatct
    31681 ggcgtgccgc ttcaaagtta tcaaaaccga cggcaatatc gaggcacggc gacttgctgt
    31741 ccatcaactc caccaccgga atccccgcca cttcaatcat ctttaaggtg cgtggcgtgt
    31801 gggtacgttc ggtgaggatc agtccatcga tattccatga gagcattgat tcgaggcgtt
    31861 cttgctccat ttccggttta taaccgtagt gcgccagcat agtttgataa ccgtgcgcgt
    31921 cggtgacgct ttcgattccg cgtaatactt ccgcgaaaac ctggttggtg agagaaggta
    31981 acaggacgcc aatcgcccgg ctggtggcat tagagaggat atcgggcgcg cgattgggaa
    32041 tataacccag ttcatcaaga gcggcggcaa tcttgccgcg tagagcgacg gaaacctgct
    32101 ccgggttgcg taaaaaacgg ctgaccgtca ttttggtcac gcctacacgg tcagccacat
    32161 cctgaagtac gggtcttttc tttttcatcg tcctgaaggt acaaaagaga tagattctct
    32221 tagtttaaca cggacgcatc agaaccttcc cggggaaaac agtgcttgtt tacaattatt
    32281 agatatacac taaatcaatt gcatcgcatt gtgctaacga cgcggcaaaa ccccggaagc
    32341 gtacatcagc acgcgaccgg agcgagtgag gaagcctgca aaaaggcagg cttcaaagat
    32401 gccgttgagt tttaaaccgg cggcagatcg aacagtaaca cttcgctgtc gctatccgca
    32461 tgaatggaga ttgcctgctc atcccagatt gccagaccat cgctggtcga ggctttcacg
    32521 ccgttaatgg tgacattgcc tttcaccacc tgaatccaga cgcggcgttc agcggcaatc
    32581 tgatgcaccg actgctcatc ttttaccagc gcccagcggt acagttccat atcctgatac
    32641 actttcagcg aaccatcacg cgcatccggc gagagcacca gctgtttgcc ctgtacggca
    32701 tcgaagcgac gctgttcata acgcggcgta ataccgtttt cttcaggcat gatccagatt
    32761 tgatacagat gcagacgctc ggtgctgctt gggttgtact ctgagtgacg aatacccgta
    32821 cccgcactca taatctggaa ctcacccgcc ggaacctgct ctttattgcc catgctgtcc
    32881 tgatgctcaa cagtaccttc cagcacgtag gtcaaaattt ccatatcttt atgcgggtga
    32941 gtgccgaagc cctgccctgc ttcaatcacg tcgtcgttaa tcacgcgcag cgcggagaag
    33001 cccataaagt tcggatcgta atagttggca aaagagaaag tatgccagga gtccagccag
    33061 ccatgatttg catgaccacg ttcatttgct ttgcgtaagt agatcatttt tattcgcccc
    33121 ctgaatgatt tcgatgggct tagtgtggac ccgatccgcc tgggatgata gagggtgaaa
    33181 attgacccct ctgttcaaaa aaattgaact attcgagggg ccatcaaact tacttcgcga
    33241 gagtcactgt ggagggagag gcttgctcaa atcctcgttc aaggatttcc agattggtaa
    33301 gaacttcaga ttccttgacg taatttggcg caccgttggt gatggtttga tacaacgcat
    33361 cataaacgcg cccgtaatcg cccatctccg gcttcatctc ttctctgacc gtcacaccct
    33421 cgtcattgac atactccagc acaccgaccg aatcatccgc tgcgaatccc ggttcgcccg
    33481 gcataatatt agccttgagg ctggtttcct gctggtcgat accgtattta ataaacgaac
    33541 ctttcttacc gtgaacgata aatttcggat aatcgatttt caccagatgg ctggttttga
    33601 cgatggcttt caggtcgcca taaaacagct gcgcttcaaa ggtgtcgtca ggattggctt
    33661 tattacgcag gctgcggatg tcataagcga cgtgatccgg gcgaccgaac agagaaataa
    33721 tctggtccat cgtatgcaca ccaagaccat agaacgcgcc atcctgcggc agcccaggtt
    33781 tggtttctgc caccgggcgg taatagtcaa aatggctttc cacttcgaca atctctccca
    33841 acttgccact ttcaatcgct tttttcgctg tcaggaagca ggaatcaaaa cgacgattct
    33901 gatacggcgt gacggtcagt cctttgcttt tcgccagcgc aaacagctcc ttcgcctggg
    33961 caagcgtcgg ggtgaacggt ttttcgacca gcacattttt cccggcttcc agcgcgcgct
    34021 tcgcgtactc gaagtggctg tctgcatggg tgcagaccac aaccagctta acatcgggat
    34081 cgtttagtac ttcgtcgaga tcgctggtga aatggatatg agagtaaatg ggggcctgtt
    34141 cttccggctt cgcatggcgg cgaaaaatat gcgcgacatg ccagctatcc ttgcggttaa
    34201 gtacatacgg cagatggtaa cgggtggtgc ttttgccgaa gccaataaag gcgcagttga
    34261 tgaccatgat ttcgtccttt ttaaggtgat tatttacacc ttagcgcaaa gcggacgtgg
    34321 ttcctactgg agtgcgcata acgaacacaa gcacttcccg tggataaatt gaaaacgaaa
    34381 gatcaaaaaa aaagccagca cccggctggc taagtaatac tggaagcaat gtgagcaatg
    34441 tcgtgctttc aggttctccg cgagggtctt cctgatcgca agacaataat aatcattctc
    34501 attcgcactt gtccaacact ttttgcaaaa aaaatgcatt tgactcgcat ttgaaagtca
    34561 atgatgttga aagggacatt taccccaaag aggacaaagg aatgagtgag atagtgatac
    34621 gccatgctga aacgcgcgat tacgaggcca tcaggcagat tcacgcccag ccggaggtgt
    34681 attgcaacac gctacaggtg cctcatcctt ccgatcatat gtggcaggag cgactcgccg
    34741 atcgtcccgg catcaagcaa ctcgtcgcct gtattgatgg agacgtcgtg ggccatctca
    34801 ccattgacgt gcaacagcgc ccacgccgca gtcatgttgc agattttggt atttgtgtcg
    34861 actcccgttg gaagaatcgc ggcgtcgcca gcgccctgat gcgagagatg attgaaatgt
    34921 gcgacaactg gttgcgggta gatcgtattg agctaacggt gttcatcgat aacgcgccag
    34981 caattaaagt ctataaaaaa tttggatttg agattgaagg gactggcaag aagtacgcat
    35041 tacgtaatgg tgaatatgtc gatgcgtatt atatggcgcg ggtgaagtaa gatagtgccc
    35101 tttttctgag atggaaaaag ggtgtcattc aaaatcggca taccttcctt taatgtattt
    35161 attttcccaa taaatatata agatttacct cataattacg tcctaaagtg taatatttta
    35221 ttttttaata tatacgccta caatttcctg gagtaaataa ataacaatta acaagcataa
    35281 tagtgccatt gataaaatag catgccataa aaggactttt catggatgag taatattgtt
    35341 tacctgacag taacgggaga acaacaagga agcatctccg caggttgtgg gacttctgag
    35401 tctacaggta atcgctggca gagcgggcat gaggatgaaa tatttacatt ctcattctta
    35461 aatagtatta
//
LOCUS       DACBVI010000034        35307 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.034, whole genome shotgun sequence.
ACCESSION   DACBVI010000034 DACBVI010000000
VERSION     DACBVI010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.034"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            267..959
                     /gene="araD"
                     /locus_tag="IU325_08110"
     CDS             267..959
                     /gene="araD"
                     /locus_tag="IU325_08110"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP5808995.1"
                     /translation="MDTLKEKVLAANLSLPLHQLVTFTWGNVSGIEREKGVMVIKPSG
                     VEYSQMGIADMVVVDLQSGEVIDGHRKPSSDTDTHLALYRAFPDIGGIVHTHSRHATI
                     WAQAGKDLTAFGTTHADYFYGIIPCTRPMKDDEINGQYEYETGRVIIETFRQREISPA
                     DIPAVLVNSHGPFAWGTDPANAVHNAVVLEEIAYMNLFTQQLHPHLTAIQQPLLDKHF
                     LRKHGANAYYGQ"
     gene            complement(1009..2382)
                     /gene="argH"
                     /locus_tag="IU325_08115"
     CDS             complement(1009..2382)
                     /gene="argH"
                     /locus_tag="IU325_08115"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HAP5808996.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVENAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(2443..3219)
                     /gene="argB"
                     /locus_tag="IU325_08120"
     CDS             complement(2443..3219)
                     /gene="argB"
                     /locus_tag="IU325_08120"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HAP5808997.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINTLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(3227..4231)
                     /gene="argC"
                     /locus_tag="IU325_08125"
     CDS             complement(3227..4231)
                     /gene="argC"
                     /locus_tag="IU325_08125"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HAP5808998.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLNLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            4385..5536
                     /gene="argE"
                     /locus_tag="IU325_08130"
     CDS             4385..5536
                     /gene="argE"
                     /locus_tag="IU325_08130"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HAP5808999.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     NLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            5838..8489
                     /locus_tag="IU325_08135"
     CDS             5838..8489
                     /locus_tag="IU325_08135"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HAP5809000.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            8672..10405
                     /gene="cptA"
                     /locus_tag="IU325_08140"
     CDS             8672..10405
                     /gene="cptA"
                     /locus_tag="IU325_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HAP5809001.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            10554..11405
                     /locus_tag="IU325_08145"
     CDS             10554..11405
                     /locus_tag="IU325_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP5809002.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(11392..11733)
                     /locus_tag="IU325_08150"
     CDS             complement(11392..11733)
                     /locus_tag="IU325_08150"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAP5809003.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(11735..12613)
                     /locus_tag="IU325_08155"
     CDS             complement(11735..12613)
                     /locus_tag="IU325_08155"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HAP5809004.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIKQIHLLPFHQYGEPKYRLLGKTWSMKEVAAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(12579..14876)
                     /locus_tag="IU325_08160"
     CDS             complement(12579..14876)
                     /locus_tag="IU325_08160"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP5809005.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAHILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASRKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14927..15247)
                     /locus_tag="IU325_08165"
     CDS             complement(14927..15247)
                     /locus_tag="IU325_08165"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAP5809006.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(15262..16341)
                     /locus_tag="IU325_08170"
     CDS             complement(15262..16341)
                     /locus_tag="IU325_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HAP5809007.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            16650..19151
                     /gene="ptsP"
                     /locus_tag="IU325_08175"
     CDS             16650..19151
                     /gene="ptsP"
                     /locus_tag="IU325_08175"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP5809008.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPRIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSEGLSCAEAIVAS
                     AHHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTGMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRQLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            19163..19825
                     /gene="fsa"
                     /locus_tag="IU325_08180"
     CDS             19163..19825
                     /gene="fsa"
                     /locus_tag="IU325_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP5809009.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDTIPGIVVKIPVTSEGLAAIKILKKE
                     GITILGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            19836..20939
                     /gene="gldA"
                     /locus_tag="IU325_08185"
     CDS             19836..20939
                     /gene="gldA"
                     /locus_tag="IU325_08185"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HAP5809010.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDDGEFDRYLMLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            21270..21887
                     /locus_tag="IU325_08190"
     CDS             21270..21887
                     /locus_tag="IU325_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HAP5809011.1"
                     /translation="MKASLALVSLLTAFTSNSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFSRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21914..22819)
                     /gene="yijE"
                     /locus_tag="IU325_08195"
     CDS             complement(21914..22819)
                     /gene="yijE"
                     /locus_tag="IU325_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HAP5809012.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSALLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSAVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKNI"
     gene            complement(22912..25092)
                     /gene="katG"
                     /locus_tag="IU325_08200"
     CDS             complement(22912..25092)
                     /gene="katG"
                     /locus_tag="IU325_08200"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HAP5809013.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGGGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(25421..26311)
                     /gene="metF"
                     /locus_tag="IU325_08205"
     CDS             complement(25421..26311)
                     /gene="metF"
                     /locus_tag="IU325_08205"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HAP5809014.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(26693..28246)
                     /locus_tag="IU325_08210"
     CDS             complement(26693..28246)
                     /locus_tag="IU325_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP5809015.1"
                     /translation="MKIKILAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIA
                     DGKRDIGGWANITTLVKQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPF
                     DAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNVFYQNSSKSFWDKPYTIIEKDGVKIG
                     VIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQS
                     SMGGTDVRRALDKDIQTASQVKGLDILITGHAYVGTPEPIKVGNTLILSTDSGGIDVG
                     KLVLDYKEKPHDFTVKNFELKTIYADEWKPDPQTKQVIDGWNKKLDEVVQQTVAKSPV
                     ELKRAYGESASLGNLAADALLVAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPF
                     PNELVTMDLTGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSSKPVGQRVITLTLNGKP
                     IEDATIYHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDYFKAGNTITDEQI
                     NGMRVKDIK"
     gene            28379..29509
                     /locus_tag="IU325_08215"
     CDS             28379..29509
                     /locus_tag="IU325_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HAP5809016.1"
                     /translation="MSTLFTINACKSFGCRNLGQPTSTDYSWPDYRLGYPALHCRACG
                     SYPPLFDEQQFRDWLSVHLSTFATEKGHFCPVCYGTKTICYGHNPQGSQRIQCRNCKK
                     VWTPKQYQKEITPPEIIETVALLVPFQGASGGQKLYVLISFDALRGNILHLSTNYTQH
                     QAGESLHYRYRGNAEPELHESNIVQRVDMREAQFLRRSQFDEIQYGSAALKRNAKGVI
                     LRPVITAHGHFRVLNILFPTVKTHVISHECFLRGAIITAWADLFRQQQGEIWFIEEEI
                     ADDTDNMPWRFQGTTYHGWWKNQWQLWVQGKNRKMVCALTGGNNSKAEMLSLATSRHF
                     IDWLHKQAVFTHSAPLSAGRVTQILLSLAQDYNNCARRLISD"
     gene            complement(29473..31905)
                     /locus_tag="IU325_08220"
     CDS             complement(29473..31905)
                     /locus_tag="IU325_08220"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP5809017.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYDIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(31908..33068)
                     /gene="metB"
                     /locus_tag="IU325_08225"
     CDS             complement(31908..33068)
                     /gene="metB"
                     /locus_tag="IU325_08225"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP5809018.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAENPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            33345..33662
                     /gene="metJ"
                     /locus_tag="IU325_08230"
     CDS             33345..33662
                     /gene="metJ"
                     /locus_tag="IU325_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HAP5809019.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            33722..34330
                     /locus_tag="IU325_08235"
     CDS             33722..34330
                     /locus_tag="IU325_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HAP5809020.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVKQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSSPQV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            34475..35150
                     /locus_tag="IU325_08240"
                     /pseudo
     CDS             34475..35150
                     /locus_tag="IU325_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1H family transposase"
BASE COUNT         8580 a         9728 c         8826 g         8173 t
ORIGIN      
        1 gctttattct gtcacctaac gaaaaattta aatacaaaaa caaaaataat cgggcatagc
       61 aatctcttcc attcatgacc gaatacgccc gaataaaacc gtttacgccc acttaccctc
      121 tttcattgcc ttcttacatg ccttgaatat tttacatttc ttatattcag cgtaatttag
      181 gcaacataac ttacgagcaa aaatagccaa aacccaaaag ttaaagttta tttatataat
      241 acatacagat tatcgtggag ttaaaaatgg atacgctaaa ggaaaaagtt cttgctgcga
      301 atttatcact accccttcat cagttagtga cgtttacctg gggaaatgtt agcggtattg
      361 agcgcgaaaa aggtgtcatg gttattaaac cttctggggt cgaatacagc caaatgggca
      421 ttgcagatat ggtggtagtg gatttacaaa gtggtgaagt tattgatgga cacaggaaac
      481 cctcttcaga cacagatact catctggcgc tataccgtgc tttcccggat attggaggta
      541 tcgttcatac ccattcccga cacgccacaa tatgggctca ggccggaaaa gatctcacgg
      601 cctttgggac aacccatgca gattatttct acggcattat cccctgtaca cgccccatga
      661 aggatgacga aattaatgga caatatgaat atgaaacggg gcgagtgatt attgaaacgt
      721 tcagacaacg agaaatatct cccgctgaca ttccggctgt tttagtaaac agtcatggcc
      781 cctttgcctg gggaactgat cccgcgaacg ccgtgcataa cgccgttgta cttgaagaaa
      841 tcgcctatat gaatctcttt acacaacagc tacaccctca tcttacggct attcaacagc
      901 ctctactgga taagcatttt ttacgcaagc atggtgccaa tgcttattac gggcagtaac
      961 cctctaagca tggcctggcg ttttgccagg ccacgaatcg cccaggactt accccaaccg
     1021 cgcctgcgca aaagcaatcg cctgcgccac ctgctgcggt gagacaccgc cttttgccgc
     1081 acgcttgtcg aggcacgatt gcagcgacag aatcggatag acatcttcgc caatcacctg
     1141 gctgaatttc tgcaactcac tgagcggcag atcttccagc ggtttgccct gacgaatggc
     1201 ttccaccacc gcttcaccca caatatgatg cgcctcgcgg aacggcacgc ctttcgccac
     1261 caggtaatcc gccagttcag tggcgttcgc atacccctgc tgcgccgctt cctggcaacg
     1321 cggacgcttc acctgaatgc cgtccagcac cagcgccgcc atatgcaggc agtccagcca
     1381 ggtatcgagc gcgtcgaaca gaccttcttt gtcttcctgc atatctttgt tgtaagccag
     1441 cggcaaacct ttcagcgtca tcatcatgcc ggttaacgcg ccctgcacac gaccgcattt
     1501 accgcgaatc agctccagcg catccgggtt tttcttctgc ggcatcaatg atgaaccaga
     1561 agtcacgcgg tcagaaagct cgacaaaccc cgcttcgccg gtgttaaaga aaatcagatc
     1621 ttcagcaaaa cgcgacagat gcaccatgcc gatagcggca gcagaaagca gttccagcac
     1681 gtggtcacgg tcagaaacgc tgtcgagact gttacgggtc gctgaggcaa atcccagcca
     1741 gcctgctaac tgttcacggt cgatttcata ggccgttccc gccagtgcgc cacagcctaa
     1801 cgggctgaca tccagacgct taagcgcatc ctgcaagcgg ctttcatcac gcgccaacat
     1861 ctcaacatag gccaggcacc agtgcgcgaa cgtcaccggc tgggcgcgtt gcaggtgagt
     1921 gtaacctggc atcaccgcgt cctgattgtt ttgtgcgttt tccaccagcg ccgattgcag
     1981 ctgccggtta gccgtcagca actcgctaac ggtatctttg caccacagtt tcaggtcagt
     2041 cgctacctga tcattacggc tacgcccggt gtgcagtttt ttgcctaact gacccacttt
     2101 gtcgatcagt ttgccttcca cccagctatg gatatcttcg gcgtcgcttt caaggatttg
     2161 ttgtggcctg gcgcgaacat cttccagcag cacgttcagc gcctcttcca gttgcgcctg
     2221 ttcttctgcg gtcagcacac cgaccgttac cagggctttg gaccaggcca cagagccaac
     2281 aatatcctgc tccgccagac ggtaatcaaa acgcagtgag tcgttgaatt gtttgaaccg
     2341 ttgatctgct gcctgggtaa aacgcccgcc ccaaagtgcc ataactctgt ttccttattt
     2401 tgaaatttaa tgccggaaag ctgcgcctcc ggctaacaaa acttaagcta aaatccgcgt
     2461 acccatcggc ataccgttaa acagtgccgg aagctgttcc gcatgacgcc aggaggcgat
     2521 atccaccggg cggcccagcg tgcgcgcagc atccagcgcc gcgttcactt tcactatcat
     2581 gccgtcagta ataatgccct gctcaatcag ttgttctgct ttcgcggcag tcatttcggc
     2641 aatgcgttgc cctttgccgt cgagaatgcc actgacgtcg gagagcaaaa tcagatccgc
     2701 gcccagcgtt gccgccagcg ccgtcgctgc ctggtcagca ttgacgttca tcagttgccc
     2761 ttcgtctgtt acgccaatgg agctgaccac cggcagataa ccgttctcca gcaatgtgtt
     2821 gataagctta ggcgaacctg gctgcgccag tccaacatgg cctaactctt catcaagctg
     2881 ggtcactttg acgctgtcgc cgtcaccgag aaacaaacct acggccgcaa tctgatgttt
     2941 cttcgcccat gccagcaggg ttttatttgc cgttcccgcc agtgctccgg tgataatgtc
     3001 aatctgatca gcaggcgtca cccgcaggcc gtttttcttt ttcaccggca gattcagccc
     3061 tttcatcagc tcatccacca cgcaaccgcc gccgtgcaca atcaccagcg gacgctgatg
     3121 cgactcacga taattcacca gtgcgctaaa cagacgttcc agcgcctctt cactatccag
     3181 cagtacgccg cccagtttga taattaatgg attcatcatt gcacccttaa ataagagact
     3241 gcgtttccgc atagccgaaa cgaatattgg cgcactgtac cgcttgtgcc gccgcgcctt
     3301 tcagtaagtt gtcttcggtc gccacaataa tcaaatgctc gccctgaacg gcaaacccga
     3361 tatcgcaaaa tggcagccca acgacatttt tcagcgccgg aacgcctttg tcatacagcc
     3421 gcaccagcgg tttatgggca tacgcctgct gtaacgcttg cgcgacttgc gcctgggtca
     3481 cacccgattt caggcggcag gtaatggttt cgagaatgcc gcgcgggaaa ttgcccagat
     3541 gtggggtgaa gataacgtca gcaccgaggt gtgtggcgat ctctggttga tggcgatgag
     3601 taaagacgcc atacggttgc aggctaactt cacaaaaact atttgaaatc gctgccttac
     3661 gtcccgcacc gctcacgccg ctggtggcgt tgatcaccgg ccactgattg aggttaagaa
     3721 gatcggcatc aatcaacggt ttcagcgcca gctgtgccgc cgtcggataa cagcccggca
     3781 ccgcaatcaa gttcgcttct tttaatttat taccgcacca ctccgccaga ccgtaggctg
     3841 cctgttccaa cagttccggg tattgatggg taaagccgta atatttttca tagaaggtgg
     3901 cgtcgttaac acgaaacgcg ccggaaaggt cgaacaccac gcagcccgct tcaagaaatt
     3961 gcggcgctaa atcgtggcta acttcatggg cggtggcgag aaacaccacg tccacccctg
     4021 ggctaaactc gctgatatcc gacatcggct gcaacggcag atcaacgatg ccttttagct
     4081 gcggatgcaa atcggagatt aactttcccg catcattgct ttgcgctgaa acagtcaaag
     4141 cggttatgtt catatgcgga tggcgattta cataggtcac tagctctgcg ccagcgtagc
     4201 cgctggcacc cacaatcagc gtattcaaca tcggggctat tcaccttctt atgtctggtt
     4261 gccaggttaa acgtaaaaca ttcaccttac ggctggtggg ttttattacg ctcaacgtta
     4321 gtgtattttt attcataaat actgcatgaa tattgatact atcatgacca gaggtgtgtc
     4381 aacaatgaaa aacaaattac cgccatttat cgagatttac cgtgctctga ttgccacacc
     4441 ttcaataagc gccacggaag aggcactcga tcaaagcaat gcagatttaa tcactctgct
     4501 ggcggactgg tttaaaaatt taggcttcaa tgtggaagtg cagcctgtgc caggaactcg
     4561 caacaaattt aatatgctgg ccagttgcgg acagggggct ggcggcttgt tgctggcggg
     4621 gcataccgat acggtgccat ttgatgacgg acgctggacg cgcgatccgt ttacactgac
     4681 ggagcatgac ggcaagcttt acggcttagg caccgccgac atgaaaggct tttttgcgtt
     4741 tatccttgat gcgctacgcg atgtcgacgt cacgaaactg aaaaaaccgc tctacattct
     4801 ggcgactgcc gatgaagaaa ccagtatggc cggagcgcgt tattttgccg aaactactgc
     4861 cctgcgcccg gattgtgcca tcattggcga accgacgtca ctacaaccgg tacgcgcaca
     4921 taaaggtcat atctctaacg ccatccgtat tcagggccag tcggggcact ccagcgatcc
     4981 agcacgcgga gttaacgcta tcgaactaat gcacgacgcc atcgggcata ttttgcaatt
     5041 gcgcgataac ctgaaagaac gttatcacta cgaagcgttt accgtgccat accctacgct
     5101 caacctcggg catatccacg gtggcgacgc ttctaaccgt atttgcgctt gctgtgagtt
     5161 gcatatggat attcgtccgc tgccaggcat gacactcaat gaacttaatg gtttgctcaa
     5221 cgatgcattg gctccagtga gcgaacgctg gccgggtcgt ctgacggtcg acgagctgca
     5281 tccgccgatc cctggctatg aatgcccacc gaatcatcaa ctggttgaag tggttgagaa
     5341 attgctcgga gcaaagaccg aagtggtgaa ttactgtacc gaagcgccgt ttattcaaac
     5401 gttatgcccg acgctggttc tggggcctgg ctcaattaat caggctcatc agcctgatga
     5461 atatctggag acgaggttta tcaagcccac ccgcgaactg atcacccagg tgattcacca
     5521 tttttgctgg cattaaaacg taggccaaac aatgtcggat gcgatgcttg cacatcttat
     5581 ccgacctaca cctttggtgt taattgagac gattttttaa cattcccata agttacgctt
     5641 atttaaagcg tcgtgaattt aatggcgtaa attcctgcta tttattcgtt tgctgaagcg
     5701 atttcgcagc atttgacgtc accgctttta cgtggcttta taaaagacga cgaaaagcaa
     5761 agcccgagca tattcgcgcc aatgcgacgt gaaggataca gggctatcaa atgataagat
     5821 ggggtgtctg gggtaatatg aacgaacaat attccgcatt gcgtagtaat gtcagtatgc
     5881 tcggcaaagt gctgggagaa accatcaagg atgcgttggg agaacacatt cttgaacgcg
     5941 tagaaactat ccgtaagttg tcgaaatctt cacgcgctgg caatgatgct aaccgccagg
     6001 agttgctcac caccttacaa aatttgtcga acgacgagct gctgcccgtt gcgcgtgcgt
     6061 ttagtcagtt cctgaacctg gccaacaccg ccgagcaata ccacagcatt tcgccgaaag
     6121 gcgaagctgc cagcaacccg gaagtgatcg cccgcaccct gcgtaaactg aaaaaccagc
     6181 cggaactgag cgaagacacc atcaaaaaag cagtggaatc gctgtcgctg gaactggtcc
     6241 tcacggctca cccaaccgaa attacccgtc gtacactgat ccacaaaatg gtggaagtga
     6301 acgcctgttt aaaacagctc gataacaaag atatcgctga ctacgaacac aaccagctga
     6361 tgcgccgcct gcgccagttg atcgcccagt catggcatac cgatgaaatc cgtaagctgc
     6421 gtccaagccc ggtagatgaa gccaaatggg gctttgccgt agtagaaaac agcctgtggc
     6481 aaggcgtacc gaattacctg cgcgaactga acgaacaact ggaagagaac ctcggctaca
     6541 aactgcccgt cgaatttgtt ccggtgcgtt ttacctcgtg gatgggcggt gaccgcgacg
     6601 gcaacccaaa cgtcactgcc gatatcaccc gccacgtcct gctactcagc cgctggaaag
     6661 ccaccgatct gttcctgaaa gatattcagg ttctggtttc tgaactgtcg atggttgaag
     6721 cgacccctga actgctggcg ctggttggcg aagaaggtgc cgcagaaccg tatcgctatc
     6781 tgatgaaaaa cctgcgttct cgcctgatgg cgacacaggc atggctggaa gcgcgcctga
     6841 aaggcgaaga actgccaaaa ccagaaggcc tgctgacaca aaacgaagaa ctgtgggaac
     6901 cgctctacgc ttgctaccag tcacttcagg cgtgtggcat gggtattatc gccaacggcg
     6961 atctgctcga caccctgcgc cgcgtgaaat gtttcggcgt accgctggtc cgtattgata
     7021 tccgtcagga gagcacacgt cataccgaag cgctgggcga gctgacccgc tacctcggta
     7081 tcggcgacta cgaaagctgg tcagaggccg acaaacaggc gttcctgatc cgcgaactga
     7141 actccaaacg tccgcttctg ccgcgcaact ggcaaccaag cgccgaaacg cgcgaagtgc
     7201 tcgatacctg ccaggtgatt gccgaagcgc cgcaaggctc cattgccgcc tacgtgatct
     7261 cgatggcgaa aacgccgtcc gacgtgctgg ctgtccacct gctgctgaaa gaagcgggta
     7321 tcgggtttgc aatgccggtt gctccgctgt ttgaaaccct cgatgacctg aacaacgcca
     7381 acgatgtcat gacccagctg ctcaatatcg actggtatcg cggcctgatt cagggcaaac
     7441 agatggtgat gattggctat tccgactcag caaaagatgc gggcgtgatg gcagcttcct
     7501 gggcgcaata tcaggcacag gatgcattaa tcaaaacctg cgaaaaagcg ggtattgagc
     7561 tgacgttgtt ccacggtcgc ggcggttcca ttggtcgtgg cggcgcacct gctcatgcgg
     7621 cgctgctgtc acaaccgcca ggaagcctga aaggtggcct gcgcgtgact gaacagggcg
     7681 agatgatccg ctttaaatat ggtctgccag aaatcaccgt cagcagcctg tcgctgtaca
     7741 ccggggcgat tctggaagcc aacctgctgc caccacctga gccgaaagag agctggcgtc
     7801 gcattatgga tgaactgtca gtcatctcct gcgatctcta ccgtggctac gtacgtgaaa
     7861 acaaagattt tgtgccttac ttccgctccg ctacgccgga acaggaactg ggcaaactgc
     7921 cgttgggttc acgtccggcc aaacgtcgcc cgaccggtgg cgttgagtca ctgcgcgcca
     7981 ttccgtggat cttcgcctgg acgcaaaacc gcctgatgct ccccgcctgg ctgggtgcag
     8041 gtacggcgct gcaaaaagtg gtcgaagatg gcaaacagag cgagctggag gctatgtgcc
     8101 gcgattggcc attcttctcg acgcgtctcg gcatgctgga gatggtcttc gccaaagcag
     8161 acctgtggct ggcggaatac tatgatcaac gtctggtaga caaagcactg tggccgttag
     8221 gtaaagagtt acgcaatctg caagaagaag acatcaaagt ggtgctggcg attgccaacg
     8281 attcccatct gatggccgat ctgccgtgga ttgcagagtc tattcagcta cggaatattt
     8341 acaccgaccc gctgaacgta ttgcaggccg agttgctgca ccgctcccgc caggcagaaa
     8401 aagaaggcca ggaaccggat cctcgcgtcg agcaggcgtt aatggtcact attgccggga
     8461 ttgcggcagg catgcgtaat accggctaat cttcccctcc tgcaaaccct cgtgcttttg
     8521 cgcgagggtt ttctgaaata cttctgttct aacaccctcg ttttcaatat atttctgtct
     8581 gcattttatt caaattctgg atatcccttc agatatcctt aaggaattgt cggtacattc
     8641 ggcgatattt ttttcaagac aggttcttac tatgcattcc acagaagtcc aggctaaacc
     8701 tctttttagc tggaaagccc tgggttgggc actgctctac ttttggtttt tctctactct
     8761 gctacaggcc attatttaca tcagtggtta tagtggcact aatggcattc gcgactcgct
     8821 gttattcagt tcgctgtggt tgatcccggt attcctcttt ccgaagcgga ttaaaattat
     8881 tgccgcagta atcggcgtgg tgctatgggc ggcctctctg gcggcgctgt gctactacgt
     8941 catctacggt caggagttct cgcagagcgt tctgtttgtg atgttcgaaa ccaacaccaa
     9001 cgaagccagc gagtatttaa gccagtattt cagcctgaaa attgtgctta tcgcgctggc
     9061 ctatacggca gtggcagttc tgctgtggac acgcctgcgc ccggtctata ttccaaagcc
     9121 gtggcgttat gttgtctctt ttgccctact ttatggcttg attctgcatc cgatcgccat
     9181 gaatacgttt atcaagaaca agccgtttga gaaaacgttg gataacctgg catcgcgtat
     9241 ggagcctgcc gcaccgtggc aattcctgac cggctattat cagtatcgtc agcaactaaa
     9301 ctcgctaaca aagctactga atgaaaataa tgccttgccg ccgctggcga atttcaaaga
     9361 tgaatcgggt aacgaaccgc gcaccttagt gctggtgatt ggcgagtcga cccagcgtgg
     9421 ccgcatgagt ctgtacggtt atccgcgtga aaccacgccg gagctggatg cgctgcataa
     9481 aaccgatccg aatctgaccg tgtttaataa cgtggtgacg tctcgtccgt acaccattga
     9541 aatcctgcaa caggcgctga cctttgccaa tgaaaagaac ccggacctgt acctgacaca
     9601 gccgtcgctg atgaacatga tgaaacaggc gggttataaa accttctgga tcaccaacca
     9661 gcagacgatg accgcccgca ataccatgct gacggtattt tcgcgccaga ccgacaagca
     9721 gtactacatg aaccagcaac gtacgcagag tgcgcgtgaa tacgacacca acgtgctgaa
     9781 gccgttccag gaagtgctga atgaccctgc gccgaagaaa ctgatcatcg ttcatctgct
     9841 gggtacgcat atcaaataca aataccgcta cccggaaaat cagggcaagt ttgatggcaa
     9901 taccgatcat gttccgccgg gattaaacgc ggaagagctg gagtcatata acgattatga
     9961 caacgctaac ctgtataacg atcatgtggt tgccagcctg attaaagact ttaaagcagc
    10021 agacccgaac ggtttcctgg tttacttctc tgaccacggt gaagaggttt acgacacgcc
    10081 gccgcacaaa actcaggggc gtaacgaaga caacccgacg cgtcatatgt acaccattcc
    10141 gttcctgctg tggacgtcgg aaaaatggca agcgactcat ccgcgtgatt tctcgcagga
    10201 tgttgatcgt aaatacagcc ttgcggaact gatccacacc tggtcagatt tggcgggctt
    10261 atcttacgac ggttacgatc caacccgttc agtggtgaat ccgcagttca aagaaactac
    10321 ccgctggatt ggtaacccgt acaagaaaaa cgcgctgatc gattacgaca cgctgccata
    10381 tggcgaccag gtaggtaatc agtaaatgac ctttgtatcg cgtctggcgc tatttgccgg
    10441 atgcgatact gccgcaatgt ttacatcgaa ctcccctcag gccacacttt tcaggtggcc
    10501 ttttttcata atgccagtta ctgttttccc gtctacatag ctaagggaat aacatgtatc
    10561 acgacgtcag ctatctgctc tcccgcctga tcaacggccc gctgtccctg cgccagattt
    10621 actttgccag cagcaacggt ccggtccccg atctcgcgta tcaggtcgat tttccacggc
    10681 tggaaattgt gctggaaggt gagtttgttg ataccggcgc tggagcaacg ttagttcccg
    10741 gcgatgtgct gtacgttcct gctggtggct ggaattttcc acaatggcaa gcccccgcca
    10801 ctacctttag cgtgctgttt ggcaaacagc aactcggctt cagcgtcgtg caatgggatg
    10861 gcaaacaata tcaaaatctg gcgaagcaac acgtcgcccg gcgcggccca cgcataggtt
    10921 cttttctgct acaaacgctc aatgaaatgc aaatgcagcc gcaggagcag caaacggcaa
    10981 ggcttatcgt cgccagcctg cttagccact gccgcgattt gcttggcagc caaatccaga
    11041 ccgcctcacg cagtcaggca ctatttgaag ctattcgtga ttatatcgac gaacgctatg
    11101 cctccgcgct tacccgcgaa tctgtcgcac aggcattcta tatttcgcca aattacctct
    11161 cgcacctgtt tcaaaaaacg ggggccattg gttttaacga atacctcaat cacacgcgac
    11221 tggagcacgc taagacgtta ttgaaaggct atgacctgaa ggtaaaagaa gtggcgcacg
    11281 cctgcggctt tgtcgacagc aactacttct gccgtctgtt tcgtaaaaat accgaacgct
    11341 caccgtcgga atatcgtcgg cagtaccaca gccaactgac agaaaaaccg actactccag
    11401 aatgagatgg gtttgctgcg gcgcagaaag cactttgcgt accgcgctca tcacccgctg
    11461 cggctcacgc aggaaagcgt agatgctgca ttgcacatag cggcaatgtt caaatcgctc
    11521 agcaccggca agctcgatat ccgtaatcaa cagcgcaaca tccgcccggc ggatatcctc
    11581 gtccgctaaa cgattctccg ttcccaacgc gccctgggtt tcaatgctga cgccccactt
    11641 ctctaactgg cacaactttt ccagccgttc cgccgccata tatgtatgcg ccacgccgct
    11701 tacgcaggcg gttactgcca ccaggtatgc cattttaacc tcccacggta acctgaaatc
    11761 cggcccgttc tgccatttca cgcatcgttg ccacatcggc tgacgacggc gcagcaacct
    11821 ctttcatcga ccatgttttc cccagcaggc ggtatttcgg ctcgccgtac tgatgaaacg
    11881 gtaacagatg gatctgcttg atattcagcg ggatcagcac gtccagcgcc tgctgcatat
    11941 tctcccggct gagcgtgaaa ccagggatca acggtaaacg cgggatcacg ttgacgccct
    12001 cactcaccag caaacgcaga ttctccagca cgcgtggcag gttcatcttc accacatccc
    12061 gcgcctgagt cgcgtccata atttttaaat cgaacaacac ttcatcgcac aatttcgcca
    12121 gcggtaacag cttgctggct ggcgcgtctc cggcagtttc aatggcacat gacacacccc
    12181 acagccgcag tcgctgtaaa aaacgggtag caaattccgc ctgcattaac acttcgccgc
    12241 cagaaagcgt gacgccgccg ccggacgtgc gaaagaaaat gtcatctttc atcacttccc
    12301 gttccagcgc gtcaaggctg atatcgcgac caatccgttc aaacgccccg gaggggcatt
    12361 catccgcatc acgcaaacat ttcgcacagt gcagacattt cgcctctctg cgtacagtct
    12421 ggattttgcc ggagatcgat tccggattag cacaccacgg gcaaagatgt ggacagcctt
    12481 taaaaaagac caccgtacga atgccctcgc cgtcattcag tgaataacgc tgaatgttga
    12541 aaatgcgcgc cacatcattg cggcgcgttt ccacaacgtt acagctgatg cgctgtccgg
    12601 cggatgatgt catcctggat ctccttcgac agttcgacaa agaaggcgct gtatccggca
    12661 acgcgcacca ccagcccggc ataatcttgt gggcgctgtt gcgcttcccg caacgtgtcg
    12721 gcgttcacca cgttaaactg aatatgttgt aacttaagct gggtaaacgc ccgtaagaag
    12781 tcggccagtt tgcgtaatcc cgcttcacct tccagggtcg ccggagtgaa tttcacgttc
    12841 agcaacgtac cgttagacag cagcgtatta tcgagcttac tgactgactt cagtaccgcc
    12901 gttggccctt gtgcgtcctg acccagcata ggtgacaagc cgccgtctgc cagctgttcc
    12961 ccggcaaaac gaccgtctgg cgtcgcgcca accaccgatc ccaacgggac gtgagccgaa
    13021 acggtatacg atcccggcgt gaagtagccg ccgcgcgggt tctggtattt ttccacttct
    13081 ttgcagtagt ggcgtaacag ttcggcgctg atgttatcca cctcgtcgat atcgttaccg
    13141 tatttctcga agcggttaat taagcgagca cggacttttt cgccttctgg cgttgcgaag
    13201 ttggctttta ataccgacag caattcgtca aaacttaaac gctgttgctc aaaaaccata
    13261 cctttcaacg catggagaga atcgctcagg ttggcgatac cgatcccctg tacgccggag
    13321 aagttataac gcgcaccgcc atcggtaata tcgcggcctt tttccagaca atcgctgata
    13381 aacgatgaga gcagcggtac aggtgcccag tcgcgatggc cgatatcaca aatattgctg
    13441 ccctctacca tcagggtgat gtagtggctg atcttggcgc ggatctgttc cagtaaacct
    13501 tcataagtca gcgcagcgtt gccttcattt tcatgcaggc agatttccat cactttcagc
    13561 aggttaaaca tcgcgatgtc atgcaagccg taggttctgc cgggaataga taattccacg
    13621 cagcccacta cggaatagtc gcgcgcatct tccagcgaaa cgccacggtt gaggaacgct
    13681 ggcaccacca cttcatcgtt aaagatttgc ggaataccgg taccgaggcg aatagtttct
    13741 gcggttttca tcaggaacgg cgtgtcgatc aaggcgttag tgcgcacccc caggttcggt
    13801 tgcggtaatt gcacactttg ataggcgtcg agacagagga acgaaagtac gttcaccgcg
    13861 ctacgtccgt tctcggttaa cccgccgagc agtgcggtat agccggtcgg gaaaccggcg
    13921 aaataacgcg cgctactggt ggagcgcaac agcacgatgt cgttgcattt cacccataaa
    13981 gattcgagca gttctttcag gaacgccgga tcttcgccct gggttaatga tgcctgatag
    14041 aacggcaaca tatactggtc gaagcgccct aaagacagag aactggcgtt ggattcgtat
    14101 tgcagaatga tgttcatgta ccagaataac tggcacgcct gccagaacgt ctgcggctta
    14161 tgttcggcgt tatggcggga gatctccgca atagtcagca gttcttcgcg acgctgggca
    14221 tctgtgcagt ttgccgccat cgtttccgcc agttcagcgt aacgcagaat gtgtttccgc
    14281 gaggcttcca gcagtaacag cgctgcctga taaaagtgat tctccggctg ttgctgacaa
    14341 tgttgctgca tctgtgccac cagctcaccc agcccgtgat tcagcaggcg cgggtaatca
    14401 ataataatgt gcccctggcc tttatccgtc tggttgatgc tgaaaatctg cgtgctggtc
    14461 gcggctttta cttcatccgt catctgcccg ttaatgaaat ctttcatcga acgtttttcc
    14521 cagtacggga acaactcttc gcggtagata cgtttatctt cttcgctgat agcaaagcgg
    14581 tcctgcggac gcgtcgggaa ttgatccagc tctttcagca gccagtaagg gtccatttcc
    14641 ggcgacataa ttccggcgcg cggtttcacc gtacggttac cggcaatcag ttcttcatca
    14701 cgaatcgaaa tttcaacatg ttcaaggata tgcgctgttg ctttcgcccg gcgcaatatc
    14761 accggttcgc cttcggtttg ccgatggctg gcggtataaa gcagcgcccg ctccagcgag
    14821 atttcacggg tattggcaaa cagtgcagtt ttgaggcgag agatacgatt cgtcatgagg
    14881 aactcctgtg ggaaagcgaa cccgccctta cgggcgggat aagaacttaa gcggtttgtg
    14941 ccaggtgagc ttcaattttg ctcataatgg catcagcgcg ttttaccgcg tcgctgatgt
    15001 tgacgcgcac gatggtcttg ccagcaaaac gctcttcaaa cttgatgccg atatctttgg
    15061 tcagaatcac catatcggcg ctcgccacgt cttccgcagt cagttcattt tccagaccaa
    15121 tcgagccctg ggtttctact ttcacttccc agcctttcgc tttcgcggca ctttccagcg
    15181 cctctgccgc catataggta tgggcaacac cagatggaca cgcggttact gcaataattt
    15241 tcgtcatggc tcgttcctca attaattaat ttcaaaatcc aaatccaggt cgtcttcttt
    15301 ttcatcagtc gaagacccat ttttacgcgc cagacttttc agcacgttca cacaaacagc
    15361 cgtcaccact gctccgacag ctactgctgc gatataaccc agcttgcctt caaccaccgg
    15421 cagcacaatc agaccgcccc agcctgcata gcactgcgca ccgaccagcg ccgcagttac
    15481 tgcaccacaa actgaaccga ccatgatgga aggaataaca cgcagcggat cggcagcggc
    15541 gaaaggaatc gccccttcag taaccccaac gcaccccatc accaacgccg ccttgccggt
    15601 ttcgcgctct tctgcggaga aatttttacg accaatcagc gtcgccagcc ccatccccag
    15661 cggcgggata caaataccca cggcagcaat ggccaccacg gtatatacac cctgagcaac
    15721 gcaaatcagc atgaaggcat aggccacttt gttaaccgga ccgcccatat cgaacgccag
    15781 catcagaccc atgatcaccg ccagcataac aatgctgccc tgctgcatcc cctgaagcca
    15841 ctgagtcagg ctgttggtca atgcccccac aggctcgccc agcccccaca tcatgatgcc
    15901 tgcggtaatc agtgtgccga cgatcggaat gatgaagatt ggcatcaccg agcgcagaac
    15961 tttatgcacc ggaattttct tcaggtaatg caccacgatg ccgccaataa tcccggcgat
    16021 cagcgcacca aagaagcccg caccaaagct gttgccgacc caggcaccga tagcgcacgg
    16081 ggccagcgca gagcgttctg caatggaata accaatgtaa gcagcgagga aaggcaccat
    16141 cagcgtcaaa cctgcaacgc cgatatcaaa cagttttttc agattcggat cggctacggc
    16201 atccggcact gcgcctttgc catacaacat gacggaaacc gccagcaaaa taccgcccga
    16261 taccacgaag ggaatcatgt gtgaaacgcc cgtcattaag tgctgacggg tatttttcag
    16321 gatctgcacc aactcattca taagctgctc ccgggcttgc aaaagcccat gtccatattt
    16381 ttgatggagg aaacaatagg caatcgcagg cggagcaaac agtggataaa aaggccattt
    16441 actggacttt tgtaatatca gtacaaaaat gcgatccgcc tcataacttg cggcaacccg
    16501 gaagcatgcc gcaaaacggc gtcttgtgag acgcgtcgca aattgacggt tacattacat
    16561 ttgtccagct tttggcagat ttgtcacatg gcaggccgtt gccgtgcgct cttatcctgt
    16621 aaacagaaaa ccgtaccggg agagaggcaa tggccctgat tgtggaattt atttgtgagc
    16681 tacctaacgg cgtacatgcg cgcccggcaa gccacgttga aacgctgtgt aatacttttt
    16741 catcacaaat tgagtggcat aacctgcgca ctgaccgcaa gggcaacgcc aaaagcgccc
    16801 ttgcgctgat tggcaccgat acgctggtgg gcgataactg ccagttactg atttccgggg
    16861 ccgacgaaca ggaagcgcac cagcgtttaa gccaatggct gcgcgatgaa ttcccccact
    16921 gcgacgcacc gctggcggaa gtaaaatctg acgaactgga accactgccg gtttcactga
    16981 ccaatctgaa tccgcgaatt atccgcgccc gcaccgtgtg cagcggtagc gcaggcggca
    17041 ttctgacgcc gatctcttct ttagatctca atgcgctggg taatcttccc gctgccaaag
    17101 gcgttgatgc cgaacaatcc gcactggaaa acggcctgac gctggtactg aaaaacatag
    17161 agtttcgtct gctggatagc gacggtgcta ccagcgcgat tctggaagcg caccgatccc
    17221 tggctggcga tacttccctg cgcgaacatt tactggcagg cgtcagcgag ggattaagct
    17281 gtgccgaagc gattgtcgcc agtgcacacc acttctgcga agagttcgcc cgttccagca
    17341 gcagctacct gcaagaacgc gccctggacg tacgcgacgt ctgcttccag ttactccagc
    17401 aaatctacgg tgagcaacgc ttcccggcac cgggcaaact gacgcagccc gccatttgta
    17461 tggctgatga actaaccccc agccagttcc tcgaactgga taaaaatcac ctcaaaggat
    17521 tgttgctcaa aagcggcggc accacctcac ataccgtaat tcttgcccgt tcgttcaaca
    17581 ttccgacgct ggttggtgtg gatattgatg cccttacccc gtggcaacat caaacgattt
    17641 atatcgacgg caacgccgga gcgattgtgg ttgagccagg ggaagccgta gcccgttatt
    17701 atcagcaaga agcccgcgta caggatgccc tgcgtgagca acagcgtgtc tggctgaccc
    17761 aacaagcccg taccgctgat ggtatccgca ttgaaattgc tgctaacatc gctcactccg
    17821 tggaagcgca ggccgcattc ggcaatggtg cggaaggagt tggtttgttc cgcactggaa
    17881 tgctctatat ggatcgcacc agcgcaccgg gcgaaagcga gctgtacaac attttttgtc
    17941 aggcgctgga gtccgccaac ggacgcagca ttattgtgcg cactatggat attggcggtg
    18001 acaaacccgt tgattatctg aacattcccg cagaggcaaa cccgttcctc ggttatcgcg
    18061 ccgtgcgtat ttatgaagag tacgcgtcgt tatttaccac acaactacgg tcgattctcc
    18121 gtgcctccgc tcacggcagc ctgaaaatca tgatcccgat gatctcttca atggaagaga
    18181 tcttatgggt gaaagaaaaa ctggcggaag ccaaacagca actgcgcaat gaacatattc
    18241 cgtttgatga gaagatccag cttggcatca tgcttgaagt gccgtcggtg atgttcatca
    18301 tcgatcaatg ctgtgaagag attgatttct ttagtattgg tagtaatgac ctgacgcaat
    18361 atctgctggc agtagatcgc gataacgcta aggttactcg tcactacaac agcctgaatc
    18421 cggcattctt acgggcgctc gattacgccg tgcaggcggt gcatcgccag ggcaaatgga
    18481 ttggtctgtg cggtgaactg ggagcgaaag gttccgtgct gccgttgctg gtcggtttag
    18541 ggctggatga gctgagcatg agcgcaccat caattccggc ggcgaaagcg cggatggcgc
    18601 aacttgatag ccgcgaatgc cgccagttgc tcaaccaggc aatggcctgc cgtacttcgc
    18661 tggaagtgga acacctgctg gcgcaattcc gcatgaccca acaggacgcg ccgctggtca
    18721 ccgccgagtg catcacgctg gaaagcgact ggcgcagcaa agaagaagtg ctgaaaggca
    18781 tgaccgataa cctgctactg gcgggccgct gccgctatcc gcgtaaactg gaagccgact
    18841 tgtgggcacg cgaggccgtt ttctctaccg gtctgggctt tagttttgcc attccccaca
    18901 gcaaatcgga acatattgag caatccacca tcagcgtagc gcgtctgcaa gcgccggtgc
    18961 gctggggcga tgatgaagca caattcatca ttatgttaac cctgaacaaa catgctgcgg
    19021 gcgatcagca catgcgcatt ttctcgcgcc tcgctcgccg catcatgcac gaagaattcc
    19081 gtaacgcgct ggttaacgcc gcctctgccg acgctatcgc cagcctgctg caacatgaac
    19141 tggaactgta aaaggaaaca tcatggaact gtatctggac accgctaacg tcgcagaagt
    19201 cgaacgtctg gcacgtatat tcccgattgc cggggtgaca actaacccga gcattatcgc
    19261 tgccagcaag gagtccatct gggaagtgct gccgcgcctg caaaaagcga tcggtgatga
    19321 gggcattctg tttgctcaga ccatgagccg tgatgcgcag ggaatggtgg aagaagcgaa
    19381 gcgcctgcgc gacaccattc cgggcattgt ggttaaaatc ccggtgactt ccgaaggtct
    19441 ggcagcaatt aaaatactga aaaaagaggg tattactata cttggcaccg ctgtatatag
    19501 cgccgcacaa gggttattag ccgcactggc aggggcaaaa tacgttgctc cgtatgttaa
    19561 ccgcgtagat gcccagggcg gagacggcat tcgtacggtt caggagctgc aagcgctgtt
    19621 agaaatgcac gcaccagaaa gcatggtgct ggcggccagc tttaaaacgc cgcgtcaggc
    19681 gctggactgt ttactggcag gatgtgaatc catcaccctg cccttagatg tagcgcaaca
    19741 aatgctcaac acccctgcgg tagagtcagc tatagagaag ttcgaacacg actggaatgc
    19801 cgcatttggc actactcatc tctaaaggag caattatgga ccgcattatt caatcaccgg
    19861 gtaaatacat ccagggcgct gatgtgatta atcgtctggg cgaatacctg aagccgctgg
    19921 cagaacgctg gttagtggtg ggtgacaaat ttgttttagg ttttgctcaa tccactgtcg
    19981 agaaaagctt taaagatgct ggactggtag tagaaattgc gccgtttggc ggtgaatgtt
    20041 cgcaaaatga gatcgaccgt ctgcgtggca tcgcggagac tgcgcagtgt ggcgcaattc
    20101 tcggtatcgg tggcggaaaa actctcgata ctgccaaagc actggcacat ttcatgggtg
    20161 ttccggtagc gatcgcaccg actatcgcct ctaccgacgc accgtgcagc gcattgtctg
    20221 ttatctacac cgatgatggt gaatttgacc gctatctgat gttgccaaat aacccgaata
    20281 tggtcattgt cgacaccaaa atcgtcgctg gcgcacctgc acgtctgtta gcggcgggta
    20341 tcggcgatgc gctggcaacc tggtttgaag cacgcgcctg ttcccgtagc ggcgcgacca
    20401 ctatggcggg cggcaagtgt acccaggcgg cgctggcact ggcagaactg tgctacaaca
    20461 ctctgctgga agaaggcgaa aaagcgatgc ttgccgccga acagcatgta gtgactccgg
    20521 cgctggagcg cgtgattgaa gcgaacacct atttgagcgg cgttggtttt gaaagcggcg
    20581 ggctggcggc ggctcacgcg gtgcataacg gcctgaccgc tatcccggac gcgcatcact
    20641 attaccacgg tgaaaaagtg gcatttggta cgctgacgca gctggttctg gaaaatgcgc
    20701 cggtggagga aatcgaaacc gtagctgcac ttagccatgc ggtaggtttg ccaataactc
    20761 tcgctcaact ggatattaaa gaagatgtcc cggcgaaaat gcgaattgtg gcagaagcgg
    20821 catgtgcaga aggtgaaacc atccacaaca tgcctggcgg cgcgacgcca gatcaggttt
    20881 acgctgcact gctggtagcc gaccagtacg gtcagcgttt cctgcaagag tgggaataac
    20941 ctactccaaa ctcccggatt gtccgggagt ttgagagtgc aggcaaagtg gctcactcgt
    21001 cactgcgggc atgtttttcc ccctcgccct ttcagggaga gggtcgggat gagggtaaat
    21061 attcgccccg gagctggcct gttcccctca ccctaaccct ctccccaaag gggagagggg
    21121 actgttcgag cactttttat acgttatcac ccgtctgaaa ctcacacgtt gttcaggaat
    21181 tttttatccg cttctggaca ttttctctac agaacaatcg ttagcccgga aggtcgaatc
    21241 tgtgactctt attttcacgg acgaaccaga tgaaagcttc actggcgctg gtcagcctgc
    21301 taacggcgtt taccagcaac tccttaaaat ctccggccgt tccaccaacc gtggtacaaa
    21361 tccaggccaa caccaacctt gccatcgcag acggtgccag acagcaaatc ggcagcacgc
    21421 tattttacga tccggcgtat gtgcagctta cctatcctgg cggtgatgtc ccgcaagaac
    21481 gcggcgtatg ttccgatgtg gtgatccgcg ccttgcgcag ccagaaggtc gatttgcaga
    21541 aactggttca cgaagatatg gcgaagaatt ttgctgagta cccgcaaaaa tggcagttaa
    21601 agcgcccgga cagcaacatc gatcaccgtc gggtgcctaa tctggaaacc tggtttagcc
    21661 gccatgataa aacacgcccc accagcaaga accccagtga ctatcaagcg ggcgatattg
    21721 tctcctggcg actggataac gggctggcgc atattggggt ggtgtcagat ggctttgccc
    21781 gcgacggtac gccgctggtg atacataaca ttggcgcagg cgcgcaggaa gaagacgtgc
    21841 tgtttagctg gcggatggtg gggcactatc ggtattttgt taaataagcg atcgccccac
    21901 atgaagaaaa aattcagatg ttttttacac tgacagcttc ttttttctta cggctcacca
    21961 gcgccagtgc cagcacaatc agcacaatac cgctaccttc aacggccccc ggattctcac
    22021 cgagcaacca ccaggaaaag agtacgccgc aaacgggaac ggccagtgtg cttaagctgg
    22081 caatactggc aggcaagttt ttcaatacaa acaaccataa gctccacgcc agcgccgtcg
    22141 ccagaatcgc actgtaggcc agagcccaga acacggtggg ctgccagtca atttcacgtt
    22201 gtggcaccag taaagcgacc gcactcatca ccagcgccgc ataaagcatc tgccaggatg
    22261 ttaacgacaa taaatccacg cgtggatggc gggcgtatag gcgtttcgcg acaatcgcgc
    22321 tcgcccccca actaacaccg gagaggattg ccagcagggc acttttcatc gaagagaaat
    22381 cgagttgcca cggctgcaac accaaaaata agccgaaagc ggcaatcagt atcgcgaaat
    22441 attgcccacg tcgcaggcgt tcaccgagaa acaacgcggc gaaaatcacc acccagaacg
    22501 gcatggtata gctcaggatc gccaccttcc ccgctcctcc gctaaccagc gcccactgcg
    22561 ccaggccaac catcccacag gtttgtaaca gggcaatggc taaggtgtat ttaaacggtg
    22621 tcgggcgcat tccgcgacca cgtaataaaa ggacaatgaa taaaacgaga gcgccgaaaa
    22681 tgcagcgtaa ggcggtgaag tcgaaggcac cgatgtagct ggtgacttgc ttcatgaaaa
    22741 tccagctata actccagata agtgtgagca caaccaggcc actgattgcc agtgggttgc
    22801 tctttcctgc ggcagacatg agagatccag tgtgtagttt tcctgtggac actatacgcc
    22861 ggtaaagaaa cttcagcgac tgccatcagg cagtcgctga agaaggtcag attacagcag
    22921 gtcgaaacgg tcgaggttca tcactttcac ccatgccgcc acgaagtctt taacaaactt
    22981 ctcgtgggca tcgctgctgg cgtaaacttc cgccaccgca cgcaggacag agttagaacc
    23041 aaagaccaga tccgcacggc tggcagtgta tttcacttcg ccggtttcgc ggtcacggcc
    23101 ttcgaacagc tctttcgatt cgtcggtcgc tttccactcg taacgcatat ccagcaagtt
    23161 cacgaagaag tcattactca atacaccaac gcggtcagtg aagacgccgt ttttgctgcc
    23221 atcgaagttg gcacccagta cacgcatccc gcccaccagc gcggtcattt ccggcgcggt
    23281 cagcgtcagt tgctgtgctt tatcaatcag cagtgactcg gtggtagaaa catccagacg
    23341 agcgcggtag ttacggaaac cgtcagcaat tggctccagc agttcgaaca tttcaatgtc
    23401 agtctgatcc tgacgcgcat caacgcgacc cggcgcaaac ggtacatgaa tgctcaaacc
    23461 tgcggcgctt gcggctttct caacgccaac cacacctgcc agcacgatga tatccgccag
    23521 cgaagctttg ccagactctt tctgaatttt ctccagaact ggcagagcac gaacggctgc
    23581 ggcgttcaca tcccagtcgc gctgcggcat taatgccaga cgtgcaccat tggcaccacc
    23641 acgtttgtcg ccaccacgga aggtagaagc agacgcccag gccaccgata ccagttcgct
    23701 gacagacaga ccagaatccg caatcgcgaa tttcagatcg ataatgtcct gctcggtcgg
    23761 gttgtagatc ggctgcggca gcggatcttg ccagatcaga tcttctttcg gtacttccgg
    23821 cccgatgtag cgagatttcg gccccatatc cctgtgcgtc agtttgaacc aggcacgggc
    23881 aaaggcttcg ttgaacgcct gcggatcgtt gaggaaacga cgagaaatct tctcgaactc
    23941 aggatcaaaa cgcagcgtca ggtcggtcac cagcattgtc ggtttacgtt tcttcgacgg
    24001 atcgaacgga tccgggataa tttccggtgc gtctaccgct tcgaactgga ttgcgccagc
    24061 cggactgcgg gtctgtaccc actcatactt gaacaggttc tcgaagaaat agttgctcca
    24121 ctgggtcggc gtctgggtcc agactacttc cagaccagag gtaatggcat ctgcgccaac
    24181 gccgctgccg taagtgctcg cccagcctaa accttgttct tcaatcggtg cagcttctgg
    24241 atcaggacct acatttgatg tcggaccggc accgtgggtt ttacccagcg tatgaccacc
    24301 cgcaatcagc gccacggttt cttcgtcgtt catgcccatg ttgccgaagg tcgcgcggat
    24361 agctgctgcc gcagaaagcg gttcgccgct gtgatccggg ccttccgggt taacgtaaat
    24421 caggcccatc tcggttgcgc ccagcggtgc tttcgccagt gcttccggat gacggtgggt
    24481 cagccaggct ttttcatcac cccagttaac atccagatcc ggttcccaga cgtcttcacg
    24541 accggcacca aaaccgaagg tacggaagcc ggagttttcc agcgccacgt tacccgcgag
    24601 gataaacagg tcggcccagg agattttctg accatatttc tgtttgattg gccacaacag
    24661 gcgacgcgct ttatcgaggc ttacgttatc cggccaggag ttcagcggtg caaaacgttg
    24721 ctgaccacga cccgcgccgc cgcgtccgtc gatggaacgg taagtacccg cgccgtgcca
    24781 ggccatacga ataaacagac cggcgtagct gccccagtcg gcaggccacc acggctgaga
    24841 ttctgtcagc agggctttca gatctttttt cagaccgtag taatctaatt tgctgaattc
    24901 tttgcggtag tcaaagtcct cacccagcgg gttagaacga ttagaatgtt ggtttaacag
    24961 gtcaacacgg agttgatttg gccaccagtc gcgcgtggtt gtgcccccac ctgcgctctg
    25021 gtcgtgaccg ccctgatgga acgggcattt gccggtggct gtcgtgttat ggatatcgtc
    25081 tgacgtgctc atcaatgtgc tcccctctac agtgttaccg ttacgataca cagcgttaga
    25141 gagaagttat aattgaatta atccacggat gcgatagttg agatcttaca tattgttggt
    25201 taaagagatg tagatcaaat tgatcgtaat taagactttt tgattatgtc cgaattcgga
    25261 caaatgcttt ataaaaaagg gttttagctt catatccttc aagtaatgag ggagaaaaca
    25321 aggctgacaa ccttattttc ccgccctcat ttcgaggcag cattttgtgc tctgtttaaa
    25381 atttgtgatc actgtgtgat tttcacaaaa gccacactat ttataaacca ggtcgaacac
    25441 ccagcgtatg gcaaatcgcg taactcattt cagcacggtt aagcgtatag aagtggaaat
    25501 ctttcactcc ttcacggctt aaaatcttca ccatatccat cgcaatattc gcgcccacca
    25561 gtttgcgggt ttcggcatcg tcatccagcc cgtcaaacat ttgcgccatc cacgccggaa
    25621 tacgcacgtt ggtcatgtcg gcaaatttct tcgcttgttt aaagttcgag accggcaaaa
    25681 ttcccggaat gatttccaca tcaatgcccg ccgatacgca gcggtcacga aaacgcaggt
    25741 agctttcgac atcaaagaag aactgagtaa tcgcgcggtt ggctccggca tccactttgc
    25801 gtttcagatt aagcaaatcc gcctgagcgc tttttgcttc cgggtgaact tccggatacg
    25861 ccgccacgga tatatcgaaa tctgccactt cttttaacag cgtcaccagg tcagaagcat
    25921 acatttctgg cttaccgctt cccggcggca gatcgccacg cagcgccacg atatgacgaa
    25981 taccgttatt ccagtagtca cgtgcaatgg tgcgcagctc gtcgggcgtc gcatcaatgc
    26041 aagtaagatg cggtgccgct tccagaccag tgcgatcttt aatgccttta ataatgctgt
    26101 gcgtacggtc gcgctcgccg gagttcgcgc cataggtcac cgatacaaac ttcggtttca
    26161 ggctgctaag gcgatcgatg gagttccaca gggtctgctc catttcactg gtgcgcggcg
    26221 ggaaaaactc gaacgaaacg ttaatctgcc cctggacttc tgccagactc tgattcaggg
    26281 catcccgctg gctggcgtga aaaaagctca taccttacct catcaatcgc ttgtagttat
    26341 atgttgtgtt gtgaacatct atccatttag acgtccagat gtaaagatga aggaaaagcc
    26401 gaggggcgtc aaccgaaaaa tgaccaatga tggtgaggaa aaatcaggga agttgaaaaa
    26461 aactcatctt aattagaggc gataaaagat ttctctatag cgcaatccac ctcgcacgtt
    26521 ttgactgata ttctgacaac acagcgacac ctgggcttat tcgaaaaaca acgtcctgcc
    26581 accggatggt gatgctaagg catctgattc ggcctgcgcc ctcgcgtagg ccgaatactg
    26641 gatctgcaaa atcaactggt aacaaacaga tgaatcaact caatacgtac ttttacttga
    26701 tatctttcac gcgcataccg ttgatctgtt catccgtgat ggtgttaccc gctttgaagt
    26761 aatcaaccac ggcgtgataa acgtaataac cgcccgttgt gttacgcgct ttcccttcgg
    26821 taaaggcggt aaaaccatct ccaccatcag caaggaaact ctgagtagca atgtggtaaa
    26881 tcgtcgcatc ttcaatgggt ttgccattca gagtcagcgt gattacccgc tgaccaaccg
    26941 gcttactgct gtcgtacttc atttccaggc ctttcgatac ctgtaaaacg ccattactta
    27001 aacttgcgcc gtgttccatc aaactgcgta attgtttacc cgtgagatcc atcgtcacca
    27061 gttcgttggg aaacgggaag gtactgatga cgccgcccat cgtaatcgcc cctgcgggga
    27121 tctcattgcg aatgccgcca gagttggtta acgccaattg ggtgttttta cccgccgcta
    27181 ccagtaaagc atcagccgcc aggttcccga gggaagccga ttcaccatag gcacgtttta
    27241 attcaaccgg cgatttcgcg accgtttgtt gcaccacttc atcaagcttt ttattccagc
    27301 cgtcgatcac ctgttttgtt tgcggatcgg gcttccactc gtcggcgtaa atggttttaa
    27361 gctcgaagtt tttcaccgta aaatcatgcg gcttctcttt gtagtcgaga accagtttgc
    27421 ccacatcaat cccgccgctg tcagtggaga ggatgagcgt attgccgact ttaatcggtt
    27481 ccggcgtacc cacatacgca tgtccggtga tcaaaatatc cagccctttc acctgacttg
    27541 ccgtctgaat atctttatcc agcgcgcgac gcacatctgt gcctcccata ctggactggc
    27601 gggccggaac accttcatgg atcagggcga cggtcaaatc aaccttgcct ttaagttcat
    27661 cgatataacg ttgtagccat ttaatttcat cacgcgcttc aatccccact cgcgttgccg
    27721 cagataccgt gtcattaaag gcaaatacac cgtgcaaacc tataacgcct attttcacgc
    27781 cgtctttttc gatgatggta tatggcttat cccagaatga tttactgctg ttctgataaa
    27841 aaacattgcc ctgcacaata gggaattttg cctgactcaa ctgtaataat gtgttgtccc
    27901 agccgtggtc aaattcatgg ttgcctatcg tgacggcatc aaacggcata gtattcataa
    27961 tatcgataat agccttacct ttggtcaggc tgctgatata tggcccggta aaataatcac
    28021 ccgcatcaaa aaaccatgtc gctttatttt ttgctttttc ttgcttaacc agcgtagtga
    28081 tatttgccca gccgccaata tcccgtttac catcagcgat ccacggcact ttataaggct
    28141 cgacatgcgc atggaggtcg ttggtataaa tgatggtgac atctttggcg caagcccaga
    28201 acggcaatgt taatgcgata cccgcagcaa gtattttaat tttcataaat agtccctttc
    28261 agtgatttaa aggattgcta cattacgttc tgaaagagtt atttatgtga gagtaatcac
    28321 atccgcaggg gatagttgcc ggacaaaatc aacgaccttg tgaaatcgtt ggtgatatat
    28381 gtcgactctg tttacgatta atgcctgcaa gtcgtttggt tgtcgtaatt tagggcaacc
    28441 aacatcgaca gattacagct ggccagacta tcgactgggt tatccggcac tgcactgtcg
    28501 ggcatgtgga agttacccgc cgttgtttga cgaacagcaa tttcgtgact ggttgtcagt
    28561 tcacttatct acttttgcaa ctgagaaagg tcatttctgc ccggtatgtt atggcacaaa
    28621 gacgatttgt tatggccaca atccacaggg gtcgcaacgc attcaatgtc gtaactgcaa
    28681 aaaagtctgg acacctaaac aatatcagaa agaaataacg cctcctgaaa taatcgaaac
    28741 ggttgctctt ctggtgccat ttcagggagc cagcggcggg cagaaactgt atgttttaat
    28801 cagttttgat gctcttcgcg gtaatattct tcatctttct actaattaca cgcagcatca
    28861 ggctggagaa agtctgcatt atcgatacag agggaatgca gaaccagagt tacacgagag
    28921 taatattgta caacgcgttg atatgcgcga agcacaattt ctacgccgca gtcagtttga
    28981 tgagattcaa tatggcagcg cagcgctcaa acgcaatgct aaaggtgtta ttctgcgtcc
    29041 ggttatcact gcacacgggc atttcagagt actgaatatt ttattcccta cagtaaaaac
    29101 acatgtaatt tcacacgagt gttttttaag aggtgccatt attacggcat gggctgatct
    29161 gtttcgccaa cagcaaggtg agatttggtt tattgaagag gaaatagctg acgataccga
    29221 taatatgccc tggcgttttc agggaacaac ttatcacggc tggtggaaaa atcagtggca
    29281 actctgggtt cagggcaaaa atcgtaaaat ggtatgtgca ttaacgggcg gaaataacag
    29341 caaagcagaa atgctctctt tagcaaccag cagacatttt atcgactggc tacataaaca
    29401 agcagtattt actcacagcg ccccgctttc cgcagggcgc gttacgcaaa tactgctttc
    29461 tctggctcag gattacaaca actgcgccag gcggttaata tccgactgaa tcgcccctgc
    29521 ggtgacgtcg cgccccgcgc caggcccacg gatcaccaga gggttatcgc gataccagcg
    29581 gctttcgatg gcaaagacgt tatcgcacgg cagcagtgat gccagcggat ggtcttcacg
    29641 caccgcttcc acgcccacac gcgctttgcc attggcatcg aaacgcgcca catagcgcag
    29701 caccagcccc atttcgcggg ccgcttccag tcgttgcacc atctgctcgt taagttcatc
    29761 gccgttttca aagaaatggt cgatgctacc gccttcgcaa tgggcaggca ccagcgattc
    29821 cacgcgcacc tgatctggtt cgatgtcgta acccgcttca cgcgccagaa tcaccagctt
    29881 gcgcatcacg tctttgccgg aaaggtcatc acgcggatca ggctcggtta agccctgctg
    29941 ccacgcctga tccaccagct cggtaaacgg cacgctaccg tcgaattgca ggaacagcca
    30001 ggagagcgtg ccggagaaga tcccactgat cgacaaaata gtatcgccgc tgtcgatcag
    30061 atcgcgcacg gtatggttga tcggcaatcc cgcaccgacg gtggcgttgt acagccagtg
    30121 acgcccggtt ttttcgaagg cgtcgtggat ctggcgatat ttattgctgt cgctggctcc
    30181 cgccagtttg ttggcgctga tgacgtggaa accgtggctg gcaaaatcga gatactgatc
    30241 agcaagctgc tggctggcgg taacgtccaa caccactaaa tcatcatacg gatgggcgcg
    30301 catccacagg aacagcgact cttcatcctg ctcgaccgct tcatcgttga agaaggctaa
    30361 cgcgcggctg gcgtccagcc cgtcatagct caacaggctg cggcggctgt ccaccacgcc
    30421 tgccagcaca aattcaaagc cggtacgcgc cgaaagcgtg ctctgctcac gggcgaacag
    30481 ttccagccag cgggaaccga tattgccctt gccgaacaat accaggccga tgcgtttttc
    30541 tgcgcggaag acggactgat gcagcccctg aatcaggctt tcggtcggtc cggtgcgcag
    30601 tactgccacc aggctgatgc cgtcatcgga ctgccaggta aactcgaccg gttggccttt
    30661 cagttgctgc cagaagcggt gacaatgcag cgggttacgg gtgacgcccg ccccgaccat
    30721 cgccaccagc gccagcccct ggcgcagacg cagttcgcca ggtaatcccg cttcgtcgag
    30781 gattttcagc gcactgtcgg ccacttctga ggtgtagcaa aattgcagca actggcgatc
    30841 gttatgcacg ccaactgcca gtggacgcac ctgcgcgcgt ttcaggatct ggtcgatctc
    30901 tttatgcgcc agtttgaaat cctggctggc gggcacctga aactcaatca aacagacatc
    30961 atcgtggctg gtgacaatac gcgcaccagt accggaggcc agcacgcgtt caatgcgcgt
    31021 ggagccttga tccggcgtgt agctacagcg cagctgcagg tcgatttcgc tgccagaaac
    31081 tggctgtaaa gtacgggcgt gaagaacggg agccgccaga cgcgccagtt cgctagcttc
    31141 atccagacgc agcaatggca gcagacaggc atctttcact ttacgcggat cggcactgta
    31201 taccccggcg acgtcgctcc agatggttac acgagaaaca cccgccagcg caccgatttg
    31261 tgtcgcggaa tagtcggaac cgttacgccc cagcagcacc gtttcaccag cgttgttacg
    31321 gctgataaac ccggtcacca ccagacgttt acccggatgt tgtaccagta gctgttgcag
    31381 caacgggtaa gaaagccctt catcaacctg cggttgcgca gcgcgttcag cgcgtaaaaa
    31441 ctcgcgggca tcaagccagg ccgctggcag cccttgttga ttaagtaccg cagacatcag
    31501 gcgcgccgac cacacttcgc cgtggcccac cacttccgca tacaccgcgt cgttaatacc
    31561 gctgtcgagc agcgccgcca ggcgttccag atcgctgaca aaagcgctaa tgaggctatc
    31621 ggcttcttcg gcgggtaaca ggccgctaat cagatcgcac tgatagcgac gcagcgtttg
    31681 ttgaacctga tgcgcagaga gacgatcggt ctggcttagt ttcaaccagt taatcaactg
    31741 gttagtggtg ctaccggcgg cggaaaccac catcatatcg tcaggctgag agtactccgc
    31801 cataatgccc gcgacacgca aataacactt cacatcagcc agactactgc caccaaattt
    31861 atgcagttga cgacctttcg cccctgcctg cgcaatcaca ctcattttta ccccttgttt
    31921 gcagcccgga agccattttc caggtcggca attaaatctt cgccatcttc aataccggtg
    31981 gagatacgca gcagcgtctc ggagatcccg gcggcggcac gcgcttctgg tgccatgcct
    32041 gcatgtgtca tggttgcggc gtgagagatt aaactttcca ctccccctaa tgattccgcc
    32101 agcgtaaaca acgacagccc gcccaggaaa cgacgcagcg tctgctcatc gccatccagt
    32161 tcaaaactca acattgcgcc aaagcctttt tgctggcgcg cggcaatttc atgcccctga
    32221 ttttccggca gcgacgggtg atacagtttt ttcaccaacg gctgggtttg cagatatttc
    32281 acaatcgcct gcgcgttgcg ctgggccagc tccatacgcg gcaccagcgt tcgcaaacca
    32341 cgtagcagca gatagctgtc aaacgcgccg cccgtcacgc caatattgtt tgcccaccag
    32401 gccagttcgg tgacaacgtc cggatcttta gcaatcacca cgccggccac cacgtctgag
    32461 tgaccgttca gatatttcgt gcatgaatgc aacaccagat cggcacctaa tgccagcgga
    32521 ttttgtaatg ccgggcttaa gaaggtgtta tccaccacgc tcaccgcccc gacttccctt
    32581 gccagatggc agattttcgc aatatccacg acgcgtaaca atggattact tgggctttct
    32641 accagtacca gtttggggtt ttctgccagc gctgcccgta atgcctgttc atcgccttga
    32701 tcaacaaaca acacgcgata gcaaccgcgt ttcgccaggc tgtcgaacag gcgatagctg
    32761 cctccgtagc agtcgtgcgg cgcaaccagc agatcgccag gtttcaaaaa gacggtcgtt
    32821 accaggtgaa tcgcggacat gccggtatta gtaagtactg caccagcacc gccttccagt
    32881 tctgccagcg cacgctgaac cacatcgcgc gttgggttgc cgcgccgcga gtaatcatgc
    32941 gcgcgcggtt cattaaatcc ggtaaagtta taggtgctgg aaagatggat gggagggaca
    33001 acgcaaccat actgttcgtc gtcatttaac ccgctacgca ctgcgatggt ggcctgttta
    33061 cgcgtcatgt gatgaagttc cctgggcttt gtcggtgaaa tgtcaggcac cagagtaaac
    33121 attgtgttaa tggacgtcaa tacatctgga catctaaact tctttgcgta tagattgagc
    33181 aaatcccaaa tagccgttaa aattatatgc attatcacgc cgacaggtgc attacacgat
    33241 gtcacggtag cgcctgtacg gtaaactatg cgggtttacg gtcagtaccc acataaattg
    33301 tgtggtctgg tctcaattta ttgacgaaga ggattaagta tctcatggct gaatggagcg
    33361 gcgaatatat cagcccatac gctgagcacg gcaagaagag tgaacaagtc aaaaagatta
    33421 cggtttccat tcctcttaag gtgttaaaaa tcctcaccga tgaacgcacg cgtcgtcagg
    33481 tgaacaacct gcgtcacgct accaacagcg agctgctgtg cgaagcgttt ctgcatgcct
    33541 ttaccgggca acctttgccg gatgatgccg atctgcgtaa agagcgcagc gacgaaatcc
    33601 cggaagcggc aaaagagatc atgcgtgaga tggggattaa cccggagacg tgggaatact
    33661 aaagcggaaa agagcggcgc ggagttaacc agccgctttt ttcatacatg gacgtttaac
    33721 gatgaaaatc aggctactga tcctcagcct gctggtttct gtgcctgcat ttgcctggca
    33781 accacaaacc ggcgacatca tctttcagat ctctcgctca tcgcaaagta aagcgatcca
    33841 actggcgacc cattccgatt acagccacac cggtatgctg gtgatacgca acaaaaagcc
    33901 ctacgttttt gaagcggtcg gcccggtgaa atacaccccg ctcaagcagt ggattgccca
    33961 tggtgaaaag ggcaaatacg ttgttcgccg cgttgaaggc ggactgagtg ttaaacaaca
    34021 gcaaaaactg gcgcaaacgg caaaacgtta tctcggcaaa ccgtatgatt tcagcttctc
    34081 gtggagcgac gatcgccagt actgttcgga agtggtgtgg aaggtttatc agaatgcgct
    34141 gggaatgcgc gtgggcgaac agcagaagtt gaaagagttc gacctgagca gcccacaagt
    34201 tcaggcaaag ctcaaagaac gttacggcaa gaatattccg cttgaggaaa cggtggtctc
    34261 gccacaagcc gttttcgatg cgccacaact caccactgtt gccaaagaat ggccgctgtt
    34321 ttcgtggtaa tggatgataa aaaaaataat tcccgccgta gtctgtttgt tgctttgtag
    34381 ttgcacgacc tttccgcaag ctgtcgctcc ggtaaacggt gatgcctcca attagttgaa
    34441 ttaaggatat aatactcgct tttaaggttg tttcatggtc acagttaacc ttcattgtcc
    34501 ccgttgccag tccgttcagg tttaccgtca cgggcagaac cccaaaagac atgatcgatt
    34561 ccgcggccgt gactgtcacc gggtatttca gctcagttac tgttacgagc cccgtaaacc
    34621 aggagttaaa gaccaaatcg ctgaaatggc tttcaacggt gccggggttc gctatactgc
    34681 ccgtacgctg aaaatcggca ttaatacagt catccgaact ttaaaaaact ctcgccacga
    34741 aaaataacgt catccccggt cgctcatgct gatgtggcac tcatctgcga acttagtcag
    34801 caatggagct ttgtcggcag taaagcgcga cagcactggc tctggtacgt gtacaacacg
    34861 aaaactggtg gtgttctggc ctacactttt ggcccccgga ctgatgaaac ctgtcgtgaa
    34921 ctgctggcgc tgcttaccct tttaccatcg gcatgttgac cactgacgaa tggggtagtt
    34981 acgctagaga gttaccaaag gagaagcatc tgactggtaa gatttttact cagcacattg
    35041 agcgtaacaa tctgaccctg agaacccgaa tcaaacgtct ggcccgcaga accatctgtt
    35101 tctcgcgttc tgtagaactt catgaaaaag tcatcggtgc ttttatcgaa aatatatgtt
    35161 ctactaattg gagtcatcac ccttctgatg agctttcctt ggatgattac cagactgaat
    35221 acacagccaa agatttttac ctcattctgc tcttttttct caaatgacat atattatatt
    35281 ttacatcata ttcataggca tatgaca
//
LOCUS       DACBVI010000035        35130 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.035, whole genome shotgun sequence.
ACCESSION   DACBVI010000035 DACBVI010000000
VERSION     DACBVI010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.035"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(239..1180)
                     /gene="fabY"
                     /locus_tag="IU325_08245"
                     /gene_synonym="yiiD"
     CDS             complement(239..1180)
                     /gene="fabY"
                     /locus_tag="IU325_08245"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HAP5809021.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1225..1662)
                     /gene="dtd"
                     /locus_tag="IU325_08250"
     CDS             complement(1225..1662)
                     /gene="dtd"
                     /locus_tag="IU325_08250"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP5809022.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1659..2531)
                     /locus_tag="IU325_08255"
     CDS             complement(1659..2531)
                     /locus_tag="IU325_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HAP5809023.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSMQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2525..3124)
                     /gene="yihX"
                     /locus_tag="IU325_08260"
     CDS             complement(2525..3124)
                     /gene="yihX"
                     /locus_tag="IU325_08260"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HAP5809024.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3223..4008)
                     /locus_tag="IU325_08265"
     CDS             complement(3223..4008)
                     /locus_tag="IU325_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP5809025.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4042..4938)
                     /gene="yihV"
                     /locus_tag="IU325_08270"
     CDS             complement(4042..4938)
                     /gene="yihV"
                     /locus_tag="IU325_08270"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HAP5809026.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDAK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATGGNLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5106..6002
                     /gene="yihU"
                     /locus_tag="IU325_08275"
     CDS             5106..6002
                     /gene="yihU"
                     /locus_tag="IU325_08275"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HAP5809027.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRSVLFGENGVCESLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6026..6904
                     /locus_tag="IU325_08280"
     CDS             6026..6904
                     /locus_tag="IU325_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HAP5809028.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRRINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLSASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            6920..8161
                     /locus_tag="IU325_08285"
     CDS             6920..8161
                     /locus_tag="IU325_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HAP5809029.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYAIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWDYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGLAPAVAAGLLDINAK"
     gene            8229..9131
                     /locus_tag="IU325_08290"
     CDS             8229..9131
                     /locus_tag="IU325_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HAP5809030.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQDYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVTAPYGVGI
                     HPYLTCNLTSVDDYLFQLPAEQVYAVDEHANPTTLHHVDELNLNFTRATKIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKTHRLFFNIHGQHN"
     gene            9192..10067
                     /locus_tag="IU325_08295"
     CDS             9192..10067
                     /locus_tag="IU325_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HAP5809031.1"
                     /translation="MDFFTLFSPDGAMVETSVVCIVIALMFLYTFVGICAGFGGGLTT
                     MPLVTLMLPVKMATPLSVIVGTATALYATWLSRKETDWRSAMVLIIFSFLGIPVGLYA
                     LSYLPDHFMKVGLGGFLILYSFYSMFIPRLPIYDRNWIAAPIGAVAGALGAAFSTNGP
                     PMVIYGMLRNLGPAAFRGTLNAFFTANNIAVIGGLATSGILTVSIFKLVLFCIPTMIL
                     GSLVGQYVHKRISVKVFRVMVFLLLIASGSMLIKGALGIPAAAALLPPFTLLVILQLA
                     FGKKVAAIRRAEESK"
     gene            10175..12211
                     /gene="yihQ"
                     /locus_tag="IU325_08300"
     CDS             10175..12211
                     /gene="yihQ"
                     /locus_tag="IU325_08300"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HAP5809032.1"
                     /translation="MDTPRPQLPDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLRISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTDSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAQTYTLKYQYLLGRDILVAPVHEQGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            12257..13642
                     /locus_tag="IU325_08305"
     CDS             12257..13642
                     /locus_tag="IU325_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5809033.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQIGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTE"
     gene            13683..15086
                     /locus_tag="IU325_08310"
     CDS             13683..15086
                     /locus_tag="IU325_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5809034.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLSIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFSLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTTP
                     SGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            15153..15845
                     /gene="ompL"
                     /locus_tag="IU325_08315"
     CDS             15153..15845
                     /gene="ompL"
                     /locus_tag="IU325_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HAP5809035.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFTPWFNLTVRNRYNHNNYSSTDLNGELDNNDTYEVGTYWNFKITEKF
                     SYTFEPHYFMRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(15937..17202)
                     /locus_tag="IU325_08320"
     CDS             complement(15937..17202)
                     /locus_tag="IU325_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5809036.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASKNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(17304..18284)
                     /locus_tag="IU325_08325"
     CDS             complement(17304..18284)
                     /locus_tag="IU325_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HAP5809037.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEEGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(18292..19002)
                     /locus_tag="IU325_08330"
     CDS             complement(18292..19002)
                     /locus_tag="IU325_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HAP5809038.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KKRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(19219..21042)
                     /gene="typA"
                     /locus_tag="IU325_08335"
     CDS             complement(19219..21042)
                     /gene="typA"
                     /locus_tag="IU325_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HAP5809039.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            21415..22824
                     /locus_tag="IU325_08340"
     CDS             21415..22824
                     /locus_tag="IU325_08340"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HAP5809040.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            22998..24047
                     /locus_tag="IU325_08345"
     CDS             22998..24047
                     /locus_tag="IU325_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HAP5809041.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            24059..25468
                     /locus_tag="IU325_08350"
     CDS             24059..25468
                     /locus_tag="IU325_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HAP5809042.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            25580..25690
                     /gene="yshB"
                     /locus_tag="IU325_08355"
     CDS             25580..25690
                     /gene="yshB"
                     /locus_tag="IU325_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HAP5809043.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(25880..27253)
                     /gene="hemN"
                     /locus_tag="IU325_08360"
     CDS             complement(25880..27253)
                     /gene="hemN"
                     /locus_tag="IU325_08360"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP5809044.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RKVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFILALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKHYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEQQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(27442..27951)
                     /gene="yihI"
                     /locus_tag="IU325_08365"
     CDS             complement(27442..27951)
                     /gene="yihI"
                     /locus_tag="IU325_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HAP5809045.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            28533..29165
                     /locus_tag="IU325_08370"
     CDS             28533..29165
                     /locus_tag="IU325_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP5809046.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(29547..32333)
                     /gene="polA"
                     /locus_tag="IU325_08375"
     CDS             complement(29547..32333)
                     /gene="polA"
                     /locus_tag="IU325_08375"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP5809047.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            32335..32577
                     /locus_tag="IU325_08380"
     CDS             32335..32577
                     /locus_tag="IU325_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809048.1"
                     /translation="MSVPVYQIMGKGCHRQDENMSFSPLFVKIFCRSSGSLANRHVDN
                     FVHKLFTSLLIYYHAFYSMMFVFVFHEVKNKTIPNN"
     gene            32697..33629
                     /locus_tag="IU325_08385"
     CDS             32697..33629
                     /locus_tag="IU325_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP5809049.1"
                     /translation="MANFLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHLQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPISDELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(33670..35100)
                     /locus_tag="IU325_08390"
     CDS             complement(33670..35100)
                     /locus_tag="IU325_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HAP5809050.1"
                     /translation="MIRKSATGVIVAFAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSHFQMQITFDNGAPDLNIKPGQKVAFDVDVEHGPLPI
                     TMLMRGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGSSFSTTLDVAP
                     AEYGKFSFGKGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDTFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         8533 a         8990 c         8586 g         9021 t
ORIGIN      
        1 ctatcaaaaa tcgctcaaac ctgaactaag atgatgaatt aagcattatc agcgataatt
       61 gccactcagc tctacaaata atatttaaat gctcctgaac agtgatgtag caagacacgt
      121 atggtgccaa cgaagaataa aaatttatag agttcttatt caatataacc ggaatagtca
      181 gagactgaag taaatgacct gaaagaatga gagaggcagc accgcccctc tccatcagct
      241 actcttcttc gttcccgccc tcttcatacg ggccaaatgg cttcgcgggc agaacgatat
      301 acgtgccttc aaacactgca cccggcgtct cgtcgccaaa gatttcgacc tgcatttgta
      361 ctcgcgcttt gcgcccgcgc gccagacggt cgagatcgcc gcttaaggca ccgaggtcgg
      421 ctaccgcatg aggtttaccg ctaatcggct tgctgtagcg aatatgcgca tccgccagaa
      481 taatcgttcc gcccagatgg cgttcacgca gcatcagcca gataagcccc caaccggtga
      541 gcgttgccag tgagaataaa ctcccggcaa acagcgtatg gtgcggattc tgattaccgg
      601 tttccggcat ggtggtgata aatttttgcc cggtatattg ctgaatgcgc acgcccattt
      661 tttcactcag cgggatatgt tcgtaccacg cctgttgcag ctgcgcgcac cagtcgccgc
      721 gatgcagaat gtcatccaga gtggcgacag gttttatcat caaaaaatgg cgaatcggcg
      781 tggtggttgg cgtggtgatc tccccttgat tgacaaaccc cagcttggcg aaaaactcca
      841 ccgcgtcttc gcgggcgcta caggtcacgc gcttaacacc ttcctgacgc gccacagatt
      901 cgagggtcat cgccatcagc gttcctaacc ctttgtcctg cacgtcggga tgaacggcca
      961 taaagcgaat ggacgcttca ttgtcggcat taatatacag tcggcctacc gccaccagat
     1021 taccctgctc gtcgacgacc atctggtgat gcgccatcgc atcccacgcg tcgcgttccg
     1081 aacctttcgg ttgatgcagg ggcttacgca acatttccca gcgaaactga tagtaacgct
     1141 ctaattcttc ttctgtttgt ggaacccgaa ggtgatacat agcgatactc tctcttgttt
     1201 cccgtgacaa ccctggaagc tggctcatac ctgcaaccag aatgtcacgg ggccatcatt
     1261 gaccagcgat acctgcatat cggcagcgaa gcgtcctgtt tgcgtgttca tctcttgctg
     1321 acggcagcgt tcgacgaaat agtcatataa cgcctctgcg cgatccggtg atgcaccttt
     1381 ggagaaactt ggacgcatcc cccgttcggt atctgcggcg agggtaaact gggaaaccac
     1441 cagtacactg ccgcccgcct gttgcacgtt gaggttcatc ttgccttcgg catcgctaaa
     1501 gatgcgatag ccgagcacac gctcgcacag acggtttgct ttctgttcgt cgtcatcctt
     1561 ttcgacaccc aataacacca aaagtcccgc gccaatttcg cccgtcactt ctccctccac
     1621 ggtgacgctg gcacgggtta cgcgttgaat taatgcaatc atggttcgtc gtcttcttct
     1681 tgttcagctg cttgttttag tttgcggtat tccccgagag tgacagtaat ttccgcacca
     1741 agcaagacga tacaccatgt ccagtagacc caaacaaaga gaatggggat caccgccagc
     1801 acaccgtaaa tgagctgata tgaggggaac atggtgatat aaagcgcgaa acctttcttt
     1861 cctgcttcga acaggagtgc ggcgacaaac gcgccgacaa tcgcgtcgcg attaggtacg
     1921 cggatagtag gaacaatgct gtacagcaac cagaaggaga tccacgacaa cagcagcgga
     1981 aaaatacgca gcacgttgtc gatgacagta ttgagatcgc tcgcccagcg cagggagagc
     2041 aaataggaac tgatcgccag actggcccct gccagcagcg gccccagcgt taaaatcatc
     2101 cagtacacgg caaacgagta aatttttggt cgcgctcgtt tactgcgcca gatggtattc
     2161 aacgcgctat cgatggagta catcaacaat aacgcggtga cgatcagccc gcacgcccca
     2221 acggcggtca tcttgttgga attggcaaca aattgttcga tataccgctg aataacatcg
     2281 ccagtagcag gcagaaagtt ggcaaaaata aagtgacgca actgcatgct gacgtcggaa
     2341 aacatgggaa aagcggcgaa aagcgcaaaa acaacggcaa caagcggcac taatgagagc
     2401 aacgaaacat aggcaaggtt acctgccagg gttgtcatgt tgtcctcatc aatgcgttgc
     2461 cagagtagtt ttagccaggc ccatagtgga cgggtacggt gcctggcttt gtcctgaatg
     2521 gtttttagca taacaccttc gcgaaatagt ccgggatggt ggttttatct ttcaccagaa
     2581 tactggtaat gcctagctga ttcgctcctt ctatattatc ggcgttatcg tcgaaaaaga
     2641 ccgtatcgct gggtgaaaaa ccttccgcct gcaaaacatg ctgataaatt cgtgcttcag
     2701 gtttgcgcat ccccagatct tgcgacagat agatatggtc agcagcatca cggatttccg
     2761 ggtattcctc cggccagaag gtagtatgca ggcggttagt attggaaagc accaccacgc
     2821 gatgcccctg ctcacgcagt ttatgcatga tggcgatcac ttccgggcgc agcgcaacaa
     2881 acaccgcctg ccagccgtgg gagaactgct catagcttag cggcagggcc atctcatgac
     2941 acagcgcctc tgcgaacgct tcgtcgctaa tttccccacg ctcatgctga tgaaacgcct
     3001 cgcccatgtg gaaactcttc ttaagcgtag ccagtggaac acgcgttaaa tcgctccagg
     3061 ctcccagcac acggttaaag tcgatatcga caatcacatt acctaaatca aagatataga
     3121 gcatttttgc ctcctttcgc gccacaagaa attaactgta gcgggaaagg atgggtttgg
     3181 ctatgtggtc aggtatgaag caaggtcaac ggtcacaaaa ttctacgcgt cttcctgggg
     3241 aaggatgatt tcaatttggc tgtcttgtaa gcgtgatttt agcgcagcgg gcggcagctc
     3301 gtcggtaaag agcgcggtaa cctgtgccac gttaccaatt tcaaccgccg ccgaagcatg
     3361 atacttagtg tgatcggcga ccagcaggat atttctcgcg tgcgccatca tcgttttcac
     3421 cacgttagct tcgttaacat caaactccat caacgcgcca tcgctctcaa tcgccccaac
     3481 gcttgttacc agataatcag cacgaaaatc agccacaaag gacgccgctg aagggccaat
     3541 gatcccgcta ttatgagagc gcaacgtacc gccgggcacc atcacttcaa agcgcgggtt
     3601 gtggtaaaga atatgcgcca cacgcaggct gttggtgatt atccgcaaat gattgtggtt
     3661 aagtaacgcc cgggcaacat gttcaacagt cgtaccgatg gtgataaata ttgtcgatcc
     3721 atcaggaata tagtctgcca ccgcttcggc aatcgctttt ttttcctcgg tttgcgaaac
     3781 ctcacgctgc tcgaacgccg tattaacgac gctggaagcc cgacccgcgc caccgtgatg
     3841 gcgcgtaatc agtccttgct cgcttaattt acgaatatcc cggcggaccg tctgcgtgga
     3901 gacatccagc agatttgcca gctcatcaat attcatatac ccacgctcgg cgatcagcat
     3961 gaggagttgg tcgtgccgcg ggttaccggt tagttcggta aggctcatga aaattcctcg
     4021 aaaaaccatc acccgggcat tttatacaaa aagtgacaaa aaagatcggg tttgatcaca
     4081 gtcagggatc ccggctcgtc caccgggacg cgtgcatttt aacgccgcca caccgctggc
     4141 gaagcgaact gactccgcta aattcccacc ggttgccaat gccaccgcca gcgcaccgtg
     4201 aaaaacatca cccgcaccgg tggtatctac cacatcaact ttgaaagccg gttgatgttg
     4261 acgcccgcca ttttccagcc agtcgcagcc ggcactcccc tgggtcacat agacatgacc
     4321 atttgtgagc gtttgtgcct gttttagcgc actggccatc tctttcacgc ccgttaagcg
     4381 cgccagaccc ggttctgaaa aggccgcgtg atcgcttaat gccaccagct cgctgatatc
     4441 ctgcggcgta atgtccccgt ccagaacggt catcacgccc gcctgccggg ccagagtgaa
     4501 ggctttttta gcaccgtcgt gccagcgtac atctgccagc acaacatccc actgagagaa
     4561 atcaatttcc tccaaccact ctgcgtcagg cagcagatcc gggctggggt agttaatgat
     4621 tatccgctcg cctttggcat ccaccatgat ggcggattgc gaagatttcg cctggttata
     4681 ccgtttggtg taacgggtgt taacgcccca ggattccagc tctgccagca ggctgttgcc
     4741 cgtgtcgtca tcaccgacgc gaccaataaa atcgacctgc gcccccagcc ttgccgccgc
     4801 aaccgctgcc gtcgccgctg gcccgccacc aacttccgta taatttctcg ccacgtattt
     4861 accgctctcc gtcggtaatc cttccacgta ataaatacga tccatcacgg taatacctac
     4921 acaagcaaca cgaatcatgg tcattcctta agcattttgg gatgcgttca ttttaatttc
     4981 accaattgtt taaaaagtga cggctgtcaa ttttttgacg ataaattaca aatacgatca
     5041 aaaacagaca agcaaagcat gtcataaatg acaaaaagtg acatcactgt attcaggaga
     5101 gggttatggc agcaatcgcg tttatcggtt taggacaaat gggttcgcca atggcgagca
     5161 atttattgca gcaagggcat caacttcgcg tctttgatgt gaacgccgaa gctgtgcggc
     5221 atctggtaga caaaggtgcg actcccgccg ccaacccggc gcaggccgct aaagatgccg
     5281 aatttatcat taccatgttg ccgaatggcg atctggtgcg cagcgtgttg ttcggtgaaa
     5341 acggcgtttg cgaaagctta tctaccgatg cgctggtcat cgatatgtcg accatccacc
     5401 cgctgcaaac cgataaattg attgccgata tgcaagccaa aggcttcagc atgatggatg
     5461 ttccggtagg ccgtacttct gcaaatgcca ttaccggtac tctgttactg ctggctggcg
     5521 gcaccgctga acaagttgaa cgcgccacac cgatcctgat ggcgatgggc agtgagttga
     5581 tcaacgcggg cggtccgggc atggggatcc gcgttaagct catcaacaac tacatgagca
     5641 tcgcgctcaa tgcgctttcg gcagaagccg ctgttttgtg cgaagccctg aatcttccct
     5701 tcgatgttgc cgtcaaggtg atgagcggca ccgccgccgg taaaggccac ttcaccactt
     5761 cctggccaaa caaagtcctc agcggggatc tttctcccgc cttcatgatc gatcttgccc
     5821 ataaggatct tggtatcgcc cttgatgtcg ccaaccagct gcatgtgcca atgccgctgg
     5881 gggccgcctc acgggaggtt tatagccagg cgcgcgcagc gggtcgcggt cgccaggact
     5941 ggtccgccat tctggaacag gtccgtgtca gtgccgggat gactgccaaa gtaaaaatgt
     6001 aacgactgga taaaggaata aatgaatgaa taagtacacc atcaacgaca ttacacgcgc
     6061 atcgggcggt tttgccatgc tggcggtcga tcaacgcgaa gccatgcgca tgatgtttgc
     6121 cgcggctggg gcacccgctc cagtagccga tagcgtttta actgatttca aagttaacgc
     6181 tgcaaaggcc ctctcgcctt atgcctcggc gattctggtg gatcagcaat tctgttaccg
     6241 ccaggtggtt gagcaaaacg cgattgccaa aagttgcgcc atgattgtcg ccgccgatga
     6301 gttcattcct ggcaacggta ttccggtaga tagcgtggtc attgaccgca gaatcaatcc
     6361 gctacaaatc aaacaagacg gtggcaaagc cttaaaactg ctggtgctgt ggcgtagtga
     6421 tgaagatgcg cagcaacgtc tggatatggt gaaagagttt aacgaactgt gccactcaca
     6481 cggcctggta agcattattg agccagtcgt ccgcccacca cgtcgtggcg ataaattcga
     6541 tcgcgaacaa gcgatcatcg atgccgccaa agaactgggc gacagtggcg ctgacctcta
     6601 caaagttgaa atgccgcttt atggcaaagg tccgcaacaa gagcttctca gtgcttcaca
     6661 acgtctgaat gaccatatca atatgccgtg ggtgatcctc tcatccggtg tcgacgaaaa
     6721 actgttcccg cgtgccgtac gcgtggcaat gacagcgggt gcatcgggct tcctggctgg
     6781 tcgtgccgtc tgggcatcgg ttgtcggttt accagacaac gagctgatgc tgcgtgacgt
     6841 ttgcgcacct aaattacaac aacttggcga tatcgtcgac gaaatgatgg ctaaacgccg
     6901 ttaagatggg gatacaaaaa tgaaatggtt taacacctta agtcacaacc gttggctgga
     6961 acaggaaacc gaccgcatct ttgattttgg taaaaattcc gtagtaccga ccggatttgg
     7021 ctggttaggc aataaagggc aaatcaaaga agagatgggc acccatctgt ggatcaccgc
     7081 tcgtatgttg cacgtttatt ccgttgctgc ggcgatgggt cgacctggcg cttactcgtt
     7141 ggttgatcac ggtatcaaag ccatgaacgg cgcactgcgc gataaaaaat atggcggttg
     7201 gtatgcctgc gtgaatgacg agggcgtggt ggatgcctcc aaacagggct atcaacactt
     7261 ctttgctctg ctgggtgctg ccagcgccgt cactaccggt cacccggaag cccgtaaact
     7321 tctcgattac gccattgaaa ttattgagaa atacttctgg agcgaagaag agcagatgtg
     7381 cctggagtcc tgggacgaag ccttcagcaa aaccgaagag taccgcggcg gcaatgccaa
     7441 catgcacgcg gtggaagcct tcctgattgt ctatgacgtc acccatgaca aaaaatggct
     7501 ggatcgcgcg attcgcgtgg cttccgtgat tatccacgac gtcgccagaa ataatcatta
     7561 tcgcgttaac gagcacttcg atacccagtg gaatccgctg ccggattaca acaaagataa
     7621 cccggcgcac cgcttccgcg cattcggtgg cacgccgggc cactggatcg aatggggccg
     7681 tttaatgctg cacatccacg cggccctgga agcccgttgt gaacagccgc ctgcctggct
     7741 gctagaagat gccaaaggtc tatttaacgc caccgtgcgc gatgcctggg cacccgatgg
     7801 cgcggacggg attgtttata ccgttgactg ggaaggcaaa ccggtggttc gcgaacgtgt
     7861 gcgttggcct atcgtcgaag cgatgggtac ggcttacgcg ctctacaccg tcaccggcga
     7921 tcgccagtat gaaacctggt atcagacatg gtgggactac tgcattaagt acctgatgga
     7981 ctatgaaaat ggttcctggt ggcaggagct ggatgcggac aataaagtca ccacgaaagt
     8041 ctgggacggc aaacaggata tttatcacct gctgcattgc ctggtgatcc cgcgtatccc
     8101 gttagcccct ggtctggcac cggctgttgc cgcaggcctg ctggacatta acgcgaaata
     8161 actgagctga aataacggca tatcaggcta catgccgggt atgtcgttat tcaataaagg
     8221 tgcctcgtat gcaaatcacc aacatgcatt gcagtggaca aaccgtttca cttgccgcgg
     8281 gggattatca cgcgactatc gtgacggttg gtgctggtct ggctgaatta acctttcagg
     8341 gttgtcattt agtcatcccg cacaaacccg aagagatgcc gctggcgcat ctgggtaaag
     8401 tgcttattcc gtggccgaac cggatcgcca acggctgtta ccgctatcag gggcaggact
     8461 atcagttacc cattaatgaa catagttcaa aagccgctat tcacgggctg cttgcctggc
     8521 gtgactggca aattagcgaa ttaaccgcca ccagcgttac cctgacagct tttctgccgc
     8581 ccagttacgg ctatccgttt atgctggctt cgcaggttgt ttattcgctc aacgctcgca
     8641 cgggcctttc cgttgagatc gcaagccaga atattggtac tgtgaccgca ccttatggtg
     8701 tgggtattca tccttattta acctgcaact taacatctgt tgatgactat cttttccagc
     8761 ttccggcaga acaggtttat gccgtcgatg agcacgcaaa tccgacaacg cttcaccacg
     8821 ttgatgagct gaatttaaat ttcacgcggg cgacaaaaat agccgcgaca aaaatagacc
     8881 atacctttaa aaccgctaat gatctgtggg aaataaccat tacccatcca caacaggcgc
     8941 tttcggtgag cctctgttcc gatcaaccct gggtgcaggt ttatagcggt gaaaaattac
     9001 agcgtcaggg actggcagtt gaacctatga gttgcccgcc taacgccttt aattcaggta
     9061 tcgacttatt attactggaa ccaggaaaaa cgcaccgcct gttttttaat attcacgggc
     9121 agcacaatta attttattaa gaaaagaacg agatcattgt ttagggttgt atttacacaa
     9181 caggaaatat tatggacttt tttacgttat tttcgccgga tggggccatg gtggaaacaa
     9241 gcgtggtatg tatcgttatc gcgttaatgt ttctttatac ctttgtgggg atatgcgcgg
     9301 gatttggcgg tggtttaacc actatgccgc tcgtcacgtt aatgctcccc gtaaaaatgg
     9361 caactccgct ctcggtgatt gtcggcaccg cgaccgcctt gtacgccacc tggctgtcac
     9421 gtaaagaaac ggattggcga tcggcaatgg tactgattat tttctcgttt ctgggaatac
     9481 ccgtcggttt atatgcgctg tcgtatttac ccgaccattt tatgaaagtg ggcttaggcg
     9541 gattccttat cctttactcg ttttacagca tgtttattcc tcgcctgccg atttatgacc
     9601 gtaactggat cgccgcacct atcggcgcag tagctggcgc attaggcgcg gcgttctcaa
     9661 ccaacggacc gccgatggtt atctacggta tgctgcgtaa cttaggcccg gcggcatttc
     9721 gtggcactct gaacgctttc ttcaccgcaa acaacatcgc ggtgatcggc gggctggcga
     9781 ccagcggaat tttgactgtt tctatcttta aactggtgct gttttgtatt cccaccatga
     9841 tcctcggttc actggttgga caatatgtgc ataaacgcat ttcagtcaaa gtgttccgtg
     9901 tgatggtctt tttgttgctg attgcctccg gttctatgtt gataaaaggc gcactgggaa
     9961 ttcccgccgc tgccgcactt ctgcctccgt ttaccttgtt agttatactg caactggcat
    10021 ttggtaaaaa agtggcggca atccgccggg ctgaagaaag taaataatca cttatgcggg
    10081 ttttaattta tacccgcaca cattctttaa ttaatgaaac ctgtaacaac aggatggcat
    10141 gaaatattag ctttatactc tatggagatt atttatggat acgccacgcc cacaattacc
    10201 agattttcag tttcatcaga ataacgacag ttttacccta cattttcaac agcgtcttat
    10261 tttaacccat agcaaagata atccttgttt atggattggt tcaggtattg cggatatcga
    10321 tatgttccgc ggcaatttca gcattaaaga taaactacag gagaaaattg cgcttaccga
    10381 cgccatcgtc agccagtcac cggatggttg gttaatccat ttcagccgtg gttctgacat
    10441 tagcgccacg ctgcgtatct ctgctgacga tcagggccgt ttattgctgg aactacaaaa
    10501 cgacaacctt aaccacaacc gcatctggct gcgccttgcc gctcaaccag aggaccatat
    10561 ctacggctgc ggcgaacagt tttcctactt cgatctgcgc ggcaaaccgt tcccgctatg
    10621 gaccagtgaa caaggcgttg gtcgcaacaa acaaacctat gtcacctggc aggccgactg
    10681 caaagaaaat gcgggcggcg actattactg gactttcttc ccacagccta cgtttgtcag
    10741 cacgcagaag tattactgcc atgttgataa cagttgctac atgaacttcg actttagcgc
    10801 cccggaatac catgaactgg cgctgtggga agacaaagct acgctacgtt ttgaatgtgc
    10861 tgacacatac atctccctgc tggaaaaatt aactgccctg ctgggacgcc agccagaact
    10921 gcccgactgg atttatgacg gggtaacgct cggcattcag ggtgggacgg aagtgtgtca
    10981 gaagaaactg gacaccatgc gtaacgcggg tgtgaaggtt aacggtatct gggcgcagga
    11041 ctggtccggg atccgtatga cctcttttgg caaacgcgta atgtggaact ggaagtggaa
    11101 cagcgaaaac tatccgcaac tggactcacg tattaagcag tggaacaaag agggcgttca
    11161 gttcctggcc tatatcaacc catatgtcgc cagcgataaa gatctctgcg aagaagcggc
    11221 acaacacggc tatctggcaa aagatgcctc tggcggtgac tatctggtgg agtttggcga
    11281 gttttacggc ggcgttgtcg atctcactaa tccggaagcc tacgcctggt tcaaggaagt
    11341 gatcaaaaag aacatgattg aactcggctg cggcggctgg atggctgact tcggcgagta
    11401 tctgcccacc gacacttacc tgcataacgg cgtcagcgcg gaaattatgc ataacgcctg
    11461 gcccgcactg tgggcgaaat gtaactacga agcccttgaa gaaacgggca agcttggcga
    11521 gatccttttc tttatgcgcg ccggttctac cgatagccag aaatactcca ctatgatgtg
    11581 ggcgggcgac cagaacgtcg actggagtct cgacgatggc ctggcgtcgg tcgtcccggc
    11641 agcgctgtcg ctggcaatga ccggacatgg tttgcatcac agtgacattg gcggttatac
    11701 caccctgttt gagatgaagc gtagcaaaga gctgctgctg cgctggtgtg atttcagcgc
    11761 cttcacgccg atgatgcgca cccacgaagg caaccgcccg ggcgacaact ggcagtttga
    11821 cggcgacgcg gaaactattg cccattttgc ccgtatgacc accgtcttca ccaccctgaa
    11881 accttacctg aaagaggccg tcgcgctgaa tgcgaagtcc ggcctgccgg ttatgcgccc
    11941 gctgttcctg cattacgaag acgatgcgca gacctacacc ctgaaatatc agtacctgtt
    12001 aggccgcgac attctggtcg ctccggtgca tgaacaaggc cgtagcgact ggacgctcta
    12061 tcttccggag gataactggg tccacgcctg gacaggtgaa gcgttccggg gcggggaagt
    12121 gaccgttaat gcgcccatcg gtaaaccgcc ggtcttttat cgcgccgata gcgaatgggc
    12181 ggcactgttc gcgtcgttaa aaagcatcta atcacgtccg cccgtgggga aactcacggg
    12241 caaagggaga acaacgatga gtcacatcac aacggaagat ccggctactt tacgtctgcc
    12301 ctttaaagag aaactctctt acggtatcgg cgatctggcc tctaacatcc tgctggatat
    12361 cggcacgctt tatcttttga agttttatac cgacgttctg gggctgcccg gcacctatgg
    12421 cggcattatc tttttgatct cgaaattctt taccgccttt accgatatgg gtaccggcat
    12481 catgctcgat tcccggcgca agatcggtcc aaaaggcaaa ttccgccctt ttattctgta
    12541 tgcatcattc ccggtcactc tgctggcgat cgccaacttt gtcggcacac cgtttgatgt
    12601 caccggtaaa acggtgatgg ccactattct gtttatgctc tacgggctgt ttttcagcat
    12661 gatgaactgc tcctacggcg caatggtccc cgctattacc aaaaacccca acgaacgcgc
    12721 atcgctggca gcatggcgtc agggtggcgc tacattgggc ttactgctgt gtacggtggg
    12781 attcgtgccg gttatgaatc ttatcgaagg taatcagcaa attggctata tcttcgctgc
    12841 cacgctgttt tcactgttcg gcctgctgtt tatgtggatc tgctactcgg gcgtgaaaga
    12901 gcgttatgtc gaaacccaac ctaccaatcc ggcgcaaaag ccaggcctgt tgcaatcttt
    12961 ccgcgcaatt gccggtaacc gcccgctgtt cattctgtgc attgccaacc tctgcacttt
    13021 aggggcgttt aacgtcaagc tcgccatcca ggtctattac acccagtacg tacttaacga
    13081 tcccatcctg ttgtcgtata tgggattttt cagcatgggc tgtattttca tcggcgtgtt
    13141 cctgatgcct ggcgcagtca gacgttttgg taagaagaag gtctatatcg gcggcctgct
    13201 gatttgggtg ctgggcgatc tgctcaacta tttcttcggc ggcggctcgg tcagctttgt
    13261 ggcgttctcc tgcctggcat tcttcggctc ggcgtttgtt aacagcctga actgggcgct
    13321 ggtttccgac accgtcgagt acggcgagtg gcgcaccggt gtgcgttcgg aaggtacggt
    13381 ctacaccggc ttcaccttct ttcgcaaagt atctcaggcg ctggcaggtt tcttcccagg
    13441 ctggatgctg acgcaaatcg gctatgtgcc aaacgtcgcc caggctgacc ataccatcga
    13501 aggtttacgc cagctgatct tcatctaccc aagcgcactg gcggtagtca ccattgttgc
    13561 gatgggttgt ttctacagtc tgaacgagaa aatgtacgtc cgcattgttg aagaaataga
    13621 agcccgtaaa cgcacggaat aaataataaa caacgccctg cggggcgtta taaggagtga
    13681 ttatgtctga ccataatcca ctgacattaa aactgaatct gcgggaaaaa atcgcctatg
    13741 gcatgggcga cgtcggttcg aatttaatgc ttagcatcgg cactctgtat ctcctcaaat
    13801 tttataccga tgaattaggg atgcctgctt actatggcgg gattatcttt ctggtagcga
    13861 agttttttac cgcgtttacc gatatgctca ccggattttt actcgactcg cggaaaaata
    13921 ttgggcctaa aggcaaattt cgtcccttta ttttatatgc agcggttccg gcggcgttaa
    13981 ttgcaacgct ccagtttatc gccaccacct ttagtttgcc ggttaaaaca acgattgcca
    14041 cagcactgtt tatgatgttt ggactttcat acagtctgat gaactgctcg tatggtgcga
    14101 tgatcccggc aattaccaaa aaccccaatg agcgcgcgca gctcgcagct taccgccagg
    14161 gtggtgcaac catagggcta ttgatttgta ccgtggcgtt tattccgttg cagtcgcttt
    14221 tttctgactc caccgtcggt tatgcctgcg cggcactgat gttctccatt ggtggcttta
    14281 tttttatgat gctgtgctac agaggcgtca aagagcatta tgtggacacg acgccaagcg
    14341 gacataaagc cagtattctc aaatcttttt gcgcgatatt tcgtaatccg ccattactgg
    14401 ttttgtgcat tgctaacctg tgtaccctgg cggcatttaa tatcaaactg gcgattcagg
    14461 tctattacac ccagtatgtg ctgaatgata ttaatttatt gtcgtggatg ggatttttca
    14521 gtatgggatg tatcctcgtc ggcgtattac tggtgccatt aactgtaaaa tgttttggta
    14581 aaaaacaggt ttatttagct ggcatggtgc tatgggcggt tggtgatatc ctgaattatt
    14641 tctggggaag taactctttc actttcgtca tgttctcttg tgtcgccttt tttggcacgg
    14701 cctttgtaaa cagcctgaac tgggcactgg tgccagatac cgtagattac ggtgaatgga
    14761 aaaccggtat tcgtgccgaa ggttctgttt ataccggtta taccttcttt cgtaaaattt
    14821 ctgccgcact tgctggcttc ttgccaggca ttatgctgac gcaaatcggt tatgttccca
    14881 acatcgcgca aagtgatgcg acattacagg gtttgcgtca gctcattttt atctggcctt
    14941 gtgcgctggc aattattgct gcattaacga tgggattctt ttacacactc aatgaaaaac
    15001 ggtttgcatt gattattgag gaaatcaacc aacgcaaaaa taaagagatg gcgacagaag
    15061 aaaaaacagc ttccgtaacg ttataaatgt caatagccgc tatttccatc ctggtgggtg
    15121 gcggcctccc tacgtttaaa aatggactta ttatgaaaaa gattaatgca ataattctat
    15181 tatcctctct gacctctgcc tcggtatttg ctggcgcata cgtcgaaaac agggaggcat
    15241 ataatctcgc ctccgatcag ggcgaagtta tgctacgcgt gggttataac ttcgatatgg
    15301 gcgcgggtat tatgttaacc aatacctaca cctttcagcg agaagatgaa ctcaaacatg
    15361 gctataatga aattgaaggt tggtatcctt tatttaaacc aaccgataaa ttaaccatcc
    15421 agcccggtgg cttaattaat gacaagagta tcggttcggg tggtgccgtt tatctggatg
    15481 ttaactacaa atttactccg tggtttaacc tgacggtgcg taatcgctat aaccataata
    15541 attacagctc tacagatttg aacggcgaat tggataataa cgacacctat gaagttggca
    15601 cctactggaa ttttaaaatc accgagaaat tctcctatac atttgaacca cattacttca
    15661 tgcgagtaaa tgactttaat agcagcaatg gaaaagatca tcactgggaa atcactaaca
    15721 ccttccgtta tcgcattaat gaacactggc tcccttattt cgaattacgc tggttagatc
    15781 gtaatgtcga accgtaccac cgcgagcaaa accagattcg tatcgggacg aagtatttct
    15841 tctgatgtaa aactgccgga tgcggcgaaa acgccttatc cggcctacgg ttgatacggt
    15901 ttccgttggc cggattaagc cacatccggc cattaattaa gcttcagccg ccagcatctc
    15961 tttcttacgt tggctaatac gcttaaccag cattacggaa accaccgcac cacagaatgc
    16021 gaagcaggcc ataatgccaa acacgatttt gtagccaata atccccggat ttaaatccag
    16081 aatgtagcca tacagactga agcagaacat cgccggggcg taaccaataa agctacccaa
    16141 cgccatcgcc gcgcctgttt tattttcagc aatttttgct tcgccgatag gtgcaaaaaa
    16201 tacggctcgc tgtgtaaaga ctatcgcgcc aaagcccagc gtacatgcca tccctaaata
    16261 taccggcata ctttcgtgcg gcagcataat caacagtacg agcgcagcgg tactgatgat
    16321 aaaggtgtag catagatatt tactcggcga tttcaggatc ttatctgaaa tcatgccacc
    16381 aatcggtcca ccaatcattt tcagacagta ttggttaatg atgccgtaag cccccaccag
    16441 cgcaacgggc aatagataga tgtttttcag gaatggaatg aagaatgtca ggccacagta
    16501 aaccgcgtaa acgaagaaga cgttgaaagc gataagccag atagttttgt ctttcagcac
    16561 cgaggtcata ctggtgtttt tcgatgctcc gtcttctttt ttcacctcaa cggacggtgc
    16621 ctcttctttg tcattcagaa caaagaaaat gataatgcct acggcaatca caataaggga
    16681 atagaaccag atgcctgctt taaaacccaa taagccactg ccaaaccagg taaatactgc
    16741 caacgcagaa aatgccacca cggtatcgac aatgccacgc cctgtttcga agaagccaaa
    16801 caaccgacct tgttgttcgc tattgcccaa tcgacttacc gacttgagca agaccggcca
    16861 gttcatcatg tcgcaagtaa cgccaaatag cgcccagaca aagaggatgc cccaataccc
    16921 cggcattgtc gtcaaataaa cacccagtaa tcctgtcgca atgagtgaga atgacatggt
    16981 gtatctgcgc ggtagtttat cggcaaaata aatagacaga aaaaagccca ctgtcgtgac
    17041 aaatgagttt accgacatag cattaccaat ttgaccattg gtcaaatgga aatattcctg
    17101 catcgggata taaaacgcat ctttcagcga cggtaattta taaattgtac cgccacacaa
    17161 tgttaataga ctaaataacg cccatttctt tttcgtgagc ataacatccc ctaagcgatt
    17221 tttaaaatca ctgttttaaa ggcaataaga taccgaccat tcatatggaa tggttttatt
    17281 aactgcgtat gagttaaaac ttattagtgg ttcagaagat ggtttgcctc ttgtttgatt
    17341 tgttgtagta cgttacgcac atgatttatc tgattaattg catcttcttt ttctttacgt
    17401 aaccgcgcat ccagtaaatg attttctggt aggggtgttt cactcatctg gcgataaggg
    17461 atccacggat tatcatcttc gtcttcaaag cggaacgccg gcgcatagta atcaacctct
    17521 tcttccaggc catatgccgt cacctgcggc tcatcatcac ccaggcagat aaggtgcgtt
    17581 aataacgctt tgaacggcat atcaccctga cctgaaatac aggctttatg acccaggcca
    17641 ccctcttctt taacgatcaa ggcatctttg atatggacct gggtaatatg cggtgccatc
    17701 gtttttaaag cgtcgatggg atgctcattt gcattgatca tgttcgcaaa atcaaacagt
    17761 aaggaaagag attccatctc actttctttg accagcgaca ccagctcatg gctttttaaa
    17821 tcttcatgct gttcgatcgt aaaagtcagg ccgctgtcct gatacgtttc ccgtacatag
    17881 gcaatgtcgt tagcgatgat cgataatacg tcgcgcaaat taccttcata acgtggataa
    17941 aaacgtacgg acgatgcccc agttttcaac gcaatggcga cggcttcgtc aatagatgcc
    18001 ttatctgaag cgctggtttc aatatgaata tcaaggttca gacggcgggc tttatcccca
    18061 aaggcagaga gttctttatc gtccatatta ccaagagaaa aacgctcgcc atccagaaca
    18121 tggattttga cgccacgtag attattttca cttgcgatat caagtaaatc agcaggcaac
    18181 actctttcca gccgcatatt taaatgaaag gcataagcat gtaaataaag aggaagagtg
    18241 tcgacacgtt gtagaatctt tcttgcgtta tttattgtca ccataaaaca cctacagatt
    18301 aatgatgagg tctacggcct caaatcgcca atattcaata tctatctgga caatattgcc
    18361 ttgctggtcg gcgcgatgct tttcaattaa gatcgctggc atacccgcag aaccgccaag
    18421 atacttgctg atatcgcccg gcattctgac gggtttgaaa atcaattttt ttactaccgt
    18481 ctctttttgg tagcactttt cccagactgc agaaaatgag tggttttcaa gttgttcaat
    18541 aaaacccgga gcaacttcag gattaatata tgtttcgtga tagaaaactt tatgtccttc
    18601 cagcgccccc cagccggtga tgcgatagag ttcagttgat ggtgtcactg caatcagtgg
    18661 cgcgagcgtc tcgggaatat cgctggtacg ttttttctcg gtaaaccccc aggaaggctc
    18721 tcgtccttgc tcaattgcgg cccgctgaaa gctcgctgac agctccggac tgtaattaaa
    18781 tcgcggctgg gtcacgaacc accccttacg atccttacgg aagattttgg attctgcctc
    18841 gaggttcaac aacgcctggc gcagcgtcat acgtttaatg cccagcagtt ctcccagctc
    18901 tctttctgaa gggagttttc cacccggcgt ggtgatgcca tctttcaacc accgatccag
    18961 tttctctttt gcattttcta cggtggattg attctccgcc attgtttaat ccttttggtt
    19021 taaaccagat gaagcatctt acgcttacgc caaaaaagaa agcaattaac ggtgtatgac
    19081 tttgcagata attgtgacaa agctctctat tgacgtaatc acagacttat aagggagata
    19141 ctcgcattgg tgtgcgctca cgacaggtaa aaaaaaacct gccagcgatg gcaggttttt
    19201 tatgactgaa aagcgaaatt aatcgtcttt cggtgcgcgg ttggcgcggc gacgatcgtt
    19261 ttccgtcagg tgacgtttac gaatacggat agaggtcgga gtcacttcta ccagttcgtc
    19321 atcatcgata aactccagag cttgttccag agtcatgcgg ataggcggaa ccagaacaac
    19381 ggcttcgtca gtaccggaag cacgcatgtt ggtcagtttc ttaccggtca ggcagtttac
    19441 agtcaggtcg ttagagcggc tatgaatacc gataatctga ccttcgtaaa cttctgcacc
    19501 gtgaccgagg aacagcttac cgcgatcctg cagaccgaac aacgcgaacg ctaccgcttt
    19561 accctgaccg ttagagatca gtacgccgtt ctgacgctga cccacttcac ccggacgtac
    19621 gtcgtcgtag tggctgaagg tggagtacag cagacccgta ccggaagtca tggtcatgaa
    19681 ctcagaacgg aagccaatca gaccacggct tgggatcacg tagtcgagac gtacgcggcc
    19741 tttaccgtct ggattcatgt ttttcaggtc gcctttacgt tcgcccagcg cctgcatcac
    19801 agaaccctga tgctgttctt caacgtccag cgtcacgttt tcatacggct cttgtttacg
    19861 accgtcgatt tcacggaaga ttactttcgg acgggatacc gccagttcga aaccttcacg
    19921 acgcatgttt tcgataagaa cagacaggtg cagttcgcca cgaccagaaa cgcggaacgc
    19981 atcggcgtct tcggtttctt ctacgcgcag cgcaacgttg tgtaccagtt ctttgttcag
    20041 acgatccagg atctgacgag acgttacgaa cttaccttct ttaccgcaga acggcgaggt
    20101 gttaacgcag aagaacatag aaacggtcgg ctcatcaacg gagagtgccg gcagcgcttc
    20161 aacgttttgc gtgtcgcaaa cggtgtcaga aatgttcagt tcgcccaggc ccgtgatcgc
    20221 aacgatatcg ccagcttccg ccagatcggt ttcaatacgt tccagaccga ggtggcccag
    20281 cactttaccg actttcgcgt tacgggtttt gccttcgcta tcgatgatag tgacctgctg
    20341 gttcggcttc actttaccgc gcttgatgcg gccaatgccg ataacgccaa catagctgtt
    20401 gtaatcgagc tgagaaatct gcatctggaa cggaccgtca aggtcaacgt ccggcgcagg
    20461 aacgtggtca acaatcgcct gatacagcgg ggtcatgtct tccgccatat cttcgtggtc
    20521 cagacccgcg ataccgttca gcgcagaagc gtaaacgatc gggaagtcca gctgctcgtc
    20581 ggtcgcgtcg aggttaacga acagatcgaa tacctgatcc acaacccaat caggacgcgc
    20641 gccagggcgg tcaactttgt tgataacaac aatcggcttc aggccgtaag caaacgcttt
    20701 tttggttacg aagcgcgttt gcggcatcgg gccgtcaaat gcgtcaacca ccagcagcac
    20761 tgagtctacc atggacatta cacgttcaac ttcaccaccg aagtcggcgt gccccggggt
    20821 atcaacgatg ttgatacggt aatcattcca tttgatagcg gtgtttttcg cgaggatggt
    20881 aatcccacgc tctttctcca aatcgttgga gtccatcacg cgctcttggg tttcggcacg
    20941 agagtcgaac gtaccggatt gttggagcag cttgtctacc agggtggttt taccatggtc
    21001 tacgtgcgcg atgatggcga tattacgcaa tttttcgatc acaactttgc ctcaggcatt
    21061 agaaatagcg cgttattgta cacggattaa tcgcactaca aaacaggatc acaaacatcc
    21121 tccgcaaaca agtattgcag agtccctttg tgatcgcttt cacggagcat aaaaagggtt
    21181 atccaaaggt cattgcacca atatggtgct taatgtttcc attgaagcac tatattggtg
    21241 caacattcac atcgtggtgc agcccttttg cacgatggtg cgcatgataa cgccttttag
    21301 gggcaattta aaagttggca cagatttcgc tttatctttt ttacggcgac acggccaaaa
    21361 taattgcaga tttcgttacc acgacgacca tgaccaatcc aggagagtta aagtatgtcc
    21421 gctgaacacg tactgacgat gctgaacgag cacgaagtga agtttgttga tttgcgcttc
    21481 accgatacca aaggtaaaga acagcacgtc actatccctg ctcatcaggt gaatgctgaa
    21541 ttcttcgaag aaggcaaaat gtttgacggc tcctcgattg gcggctggaa aggcattaac
    21601 gaatccgaca tggtgctgat gccagacgca tccaccgcag tgattgatcc gttcttcgcc
    21661 gattctaccc tgattatccg ttgcgacatc cttgaacctg gtaccctgca aggctatgac
    21721 cgtgacccgc gctccatcgc gaagcgcgct gaagattacc tgcgttccac tggcattgcc
    21781 gacaccgtac tgttcgggcc agaacctgaa ttcttcctgt tcgatgacat ccgtttcgga
    21841 tcttctatct ccggttccca cgttgctatc gacgatatcg aaggcgcatg gaactcctcc
    21901 acccaatatg aaggtggtaa caaaggtcac cgtccggcag tgaaaggcgg ttacttcccg
    21961 gttccgccgg tagactctgc tcaggatatt cgttctgaaa tgtgtctggt gatggaacag
    22021 atgggtctgg tggttgaagc ccatcaccac gaagtagcga ctgctggtca gaacgaagtg
    22081 gctacccgct tcaataccat gaccaaaaaa gctgacgaaa ttcagatcta caaatatgtt
    22141 gtgcacaacg tagcgcaccg cttcggtaaa accgcgacct ttatgccaaa accgatgttc
    22201 ggtgataacg gctccggtat gcactgccac atgtcgctgt ctaaaaacgg cgtcaacctg
    22261 ttcgcaggcg acaaatacgc aggtctgtct gagcaggcgc tgtactatat tggcggcgta
    22321 atcaaacacg cgaaagcgat taatgccctg gcaaacccga ccaccaactc ttacaagcgt
    22381 ctggtcccgg gctacgaagc accggtaatg ctggcttact ctgcacgcaa ccgttctgcg
    22441 tctatccgta ttccggtggt ttcttctccg aaagcacgtc gtatcgaagt acgtttcccg
    22501 gatccggcgg ctaacccgta cctgtgcttt gctgccctgc tgatggccgg tcttgatggt
    22561 atcaagaaca agatccatcc gggcgaagcc atggacaaaa acctgtatga cctgccgccg
    22621 gaagaagcga aagagatccc acaggttgct ggttctctgg aagaagcact gaacgaactg
    22681 gatctggacc gcgagttcct gaaagccggt ggcgtgttca ctgatgaagc gatcgatgcg
    22741 tacatcgctc tgcgtcgcga agaagatgac cgcgtgcgta tgactccgca tccggtagag
    22801 tttgagctgt actacagcgt ctaatagttg aagttgtact acccggcgca acaacgccgg
    22861 gatttagttg ccgtggaaac tttcagccca tctctgcatg ggcttttttc tccgtcaatt
    22921 ctctgatgct tcgcgctttt tatccgtaaa aagctataat gcactaaaat ggtgcaacct
    22981 gttcaggaga ctgctttatg gcaacaggca cgcagcccga tgctgggcag atcctcaact
    23041 cgctgattaa cagtattttg ttaatcgatg acaacctggc gatccattac gccaaccccg
    23101 ccgcgcaaca actgctcgcc caaagctccc gcaaattgtt tggtacaccg ttaccggaac
    23161 tgttgagcta cttctcatta aatatcgagc tgatgcaaga aagtctggag gcggggcaag
    23221 gttttactga taacgaagtg acgctggtca tcgacgggcg ttcgcatatc ctttctgtga
    23281 cggcccagcg tatgccggac ggcatgatcc tgctggagat ggcaccgatg gataaccagc
    23341 gccgcttaag tcaggaacag ctacagcacg cccagcaggt tgctgcccgt gatttagtgc
    23401 gcggcctggc acatgagatt aaaaatccgc ttggcggttt acgtggcgcg gcgcagctgc
    23461 tcagcaaagc attacctgac ccgtcactac tcgaatatac caaagtgatt atcgaacagg
    23521 cggaccggct gcgaaatctg gtcgaccgtc tgttgggacc gcaactgccc ggtacgcgcg
    23581 ttaccgaaag tattcataaa gtggctgaac gcgtggtgac gctggtgtcg atggaactgc
    23641 cggacaacgt gcggttgatt cgtgattacg accccagcct gccggaactg gcgcacgacc
    23701 cggatcaaat tgagcaagtg ctcctgaata ttgtgcgcaa tgcgctacag gcgctggggc
    23761 cagagggtgg tgaaatcatt ctgcgtaccc gcacagcttt ccaactgacc ttacacggcg
    23821 agcgttatcg gctggcggcg cgaattgatg tggaagataa cgggccaggt attccgcctc
    23881 atttgcagga tacactgttt tacccgatgg tcagcggccg cgaaggtggc accgggcttg
    23941 gcttatccat cgcccgtaat ttgattgatc agcattcagg caaaattgaa tttaccagtt
    24001 ggccaggtca taccgagttc tcggtttacc tgcctatcag gaaataaagg tgacgtttat
    24061 gcaacgaggg atagtctggg tagtcgatga cgatagttcc atccgttggg tgcttgaacg
    24121 tgcgctcgct ggagcgggtt taacctgtac aacatttgag aacggcgcgg aggtactgga
    24181 ggcgctggcg agcaaaacgc cggatgtgct gctttcagat atccgtatgc cgggaatgga
    24241 cgggctggcg ctgctcaagc agattaaaca gcgccatccg atgcttccgg tcatcattat
    24301 gaccgcacat tccgatctgg atgctgccgt cagcgcctat caacaagggg cgtttgatta
    24361 tctgcccaaa ccgtttgata tcgacgaagc cgtggcgctg gttgagcgcg ccatcagcca
    24421 ttatcaggaa cagcagcagc cgcgtaatgt tcagcttaac ggcccaacga ccgatatcat
    24481 cggcgaagcg ccagccatgc aggacgtgtt ccgtattatc ggtcggcttt cgcgttcttc
    24541 tattagcgtg ctgattaacg gcgaatccgg caccggtaaa gaactggtcg ctcatgccct
    24601 gcatcgccac agtccgagag ccaaagcgcc atttatcgcg ctgaatatgg cggctatccc
    24661 gaaggatttg atcgaatcag aactgtttgg tcacgagaaa ggcgcgttta ccggcgcgaa
    24721 taccattcgt caggggcgtt ttgaacaggc cgatggcggc acgttatttc tcgacgaaat
    24781 tggtgatatg ccgctggatg tgcagacgcg tttgctgcgc gtgctggcag acggtcagtt
    24841 ttatcgcgtt ggcggctatg cgccggtgaa agtggatgtg cggattatcg ctgccactca
    24901 ccagaatctt gaacagcggg tgcaggaagg taagttccgt gaggatctgt tccaccgcct
    24961 gaacgtcatc cgcgttcatc tgccaccact gcgtgagcgt cgggaagata ttccccgcct
    25021 ggcacgccat tttttacagg ttgccgcgcg cgaactgggc gtagaagcga agttactgca
    25081 tccggaaacc gaagccgcgc tgacgcgtct ggcgtggcca ggcaacgtgc gccagctgga
    25141 aaacacctgc cgctggctaa cggtaatggc tgccggacag gaagtgttga ttcaggattt
    25201 gcctggcgaa ctgtttgaat caacggttgc ggagagtact tcgcaaatgc aaccggacag
    25261 ttgggcgacg ctgttagcgc agtgggcaga cagggcgctg cgttccggtc atcaaaatct
    25321 gctttccgaa gcgcagcctg agctggagcg gacgttactg accaccgcgc tgcgacatac
    25381 gcaggggcat aaacaggaag cggcgcggct actcggctgg gggcgtaaca ctctgacgcg
    25441 gaagttaaaa gagctgggga tggagtgatt cacggcttgt gtgtaaagat tgattattga
    25501 gcgcaaattg ctggtatttt gcgctttact gttccgataa gttcagtatg atcttgcccg
    25561 gaaaacgggg agagtcatta tgctggaatc aataattaat ctggtatcga gtggcgcagt
    25621 tgacagccac acaccgcaaa ctgccgttgc tgccgtgctg tgtgccgcaa tgattgggct
    25681 gtttagttga gtttcagatt gctgatgaag tgctctttgt tcatgccgga tgcggcgtga
    25741 acgccttatc cggtctacaa aagcaatatt ttcaacaggt tttgtaggcc tgataagcgt
    25801 agcgcatcag gcaatcttgt tctcagcaca ttgatggcgc tttcgttttc tactttgtaa
    25861 acgaagcgcc attcactatt taaatcaccc gagagaactg ctgcatccgc gctttctggc
    25921 gcagataggt atcaaagcac atgcaaatgt tgcggatcag caagcgacct ttcgccgtca
    25981 cctgaatccc cttctcatcc acatccacca gcccatcttt tgctaacggg gcgagcagct
    26041 tgagatcttc cgcaaagtaa tcagcgaagt gcaaatccca ctgttgctca atgggagcgt
    26101 aatccagacg gaagttgcag atgagcgact taatcacatc gcggcgaata cagtcatcac
    26161 gcgttagcgc aataccacgc cacagcgcat tgccttgttc atccacttgc tgatagtagt
    26221 gcttcaactc tttctggttc tgcgcatagc agtcgccaat catactgatg gcggaaacgc
    26281 ccatccccag cagatcggta tcgccctgag tggtgtagcc ctggaagtta cggtgcagca
    26341 cgccttcacg ctgggccacc gccagctcgt catccggacg ggcaaagtga tccataccga
    26401 taaactgata gcccgattgc gtcaggaagg cgatggtttc ctgcaggata tcgagttttt
    26461 gctgcggact cggcaggtca gcatctttga ttttgcgctg agcagcaaaa atggtcggca
    26521 gatgcgcgta gttaaagaca ctcagacgat cggggttcag ctccgccacg cgtttcaggg
    26581 taaaagcgaa actctccggc gtttgtttcg gcaggccgta aatcaggtcg atgttggtgg
    26641 aggtaaagcc aatctcacgc gcatggttaa gcagtgccaa gatgaattct tcatcctgct
    26701 cgcggttaac cagacgttgc acttctttgt tgaagtcctg cacgcccata ctcagacgat
    26761 taaagccctc ggcgcgtaaa tgatcgagta catccagttc gatttcgcgc ggatcgactt
    26821 cgatcgaaat ctccgcatcg gcattgaact ggaagttttc gcgcagcagc ttcatcagac
    26881 ggctgatttg cgctttgttc agatacgtcg gcgtaccacc gccccagtgc agctggctca
    26941 ccttgcgccc ggcaaaaagc ggtgcacgat ggacgatttc ttgctccagc gcgtccagat
    27001 actgatcggc cttgtgctgc tggcgagtaa caatcttatt gcaaccgcag aagtaacaaa
    27061 gcttatggca gaacgggata tgtacgtaga gagataatgg acgctcagga tagcgcgcca
    27121 cggcttgtaa aaacgcctgt tcgccgaagt cttctgaaaa ctccagcgcg gtcgggtacg
    27181 aggtgtatcg tggcccggaa tagttatatt tctggatcag ggccagatcc cagtcgattt
    27241 gctgtacaga catgctcact ccttccgatg gcgtctctgg cggctacggc gtaccggcgg
    27301 atgcccgttt cgggtcatca gccggttgcg cagccacttt tgtcgccgcg acaaccgctg
    27361 tagtttaacg aataaccaca ccagataaca tataaccgga agggttatca gcagaacggg
    27421 aagacccacg gcggcaatcc gttagttgcc ccgcagcaga cgcatcatgt cttcttgctt
    27481 ctcgtcttct tcctcttctt cgtcatcgtc ataagagagg ccgagtttct gcatcaactc
    27541 atcaatacga tccagtttgg catccaccca ggattgctct tcggcgctca gggtttcgcc
    27601 tgcttccaga cgttccagca gcgcatccag acgctcatcc gtttccagta actccaactc
    27661 cgcctgcggt gaaagcatag gtttctcact cttcggtttg tgctgtttgg tgactttttc
    27721 agtcacgccc aatggaatag gggttttact gccaatacgt ggatctttcg gtgcgttctg
    27781 gcctttactg cctgacgtgg tgttaccgcc cgctgcgcgg ctgcccggcg catgaccacg
    27841 acgttttttc tgacgcttac ggtcacgagc ttcctgatcc agctcctcgc gtgtttttcg
    27901 acgcgctttt gcatgacctt tgctgcgtga gtttgaagat gatggtttca taatggttcg
    27961 tcttttagcc gttttattca gtatagattt gcggcggaat ctaacagaaa gcaagcaaag
    28021 aaaaaaggcg acagattact ctgtcgcctt ttttcctgac tcataaccct taacgggtct
    28081 tacaatcctt gcaggccgcc aacacaccct gtttttccat tagccggaac aatccgtgtt
    28141 gattccattt ccgtttaatt acgtctccgg acgtttgtct tcctgacaat cctctgtctt
    28201 cgcctcctgg cgctcctgac cctcatcctg agtcattgtt cctgttagcg tcctcgctct
    28261 atgggtgcta ctttacgcct ttgctttaaa caaacaatca accgccatca gtatttgcac
    28321 attttcagag ttttatataa aagcaattaa ctgatttata tagcttattt gacatgcatc
    28381 gcagaatttt ctctgctatt tcgcataaag actatcggca atgtctcaca aagcgtatgg
    28441 cttttactta caacgctcac gccgaaaaat ccgcctttta ttatggttca ggtataatcg
    28501 ccgcaaagct ttgactgacg gagacgaccg ctttgactaa tttgaattat caacagacgc
    28561 attttgtgat gagtgcgcct gatattcgcc acctaccttc cgataccgga attgaagtgg
    28621 cttttgcagg ccgttccaac gcaggtaaat ccagcgcgct gaacacgctg actaaccaga
    28681 aaagcctggc tcgtacctca aaaaccccag ggcgcaccca gcttatcaac ctgtttgaag
    28741 tggctgacgg caagcgtctg gttgacctgc ccggctatgg ctacgcggaa gtcccggaag
    28801 agatgaagcg caaatggcaa cgcgcgctcg gcgaatacct cgaaaaacgt cagagcctgc
    28861 aaggtctggt ggtgctaatg gatattcgcc atccgctgaa agatttggat caacagatga
    28921 ttgagtgggc ggtagacagc aatatcgccg tgctggtgct gctgaccaaa gcggacaaac
    28981 tggcaagcgg cgcacgtaaa gcgcaattga atatggtgcg tgaagcggtg ctggcgttta
    29041 acggtgatgt gcaggttgaa acgttctctt cactgaagaa acaaggcgtg gacaagctgc
    29101 ggcagaaact ggatacctgg tttagcgaga tgcagcctgt agaagaaacg caggacggcg
    29161 aataattttc ttgccttaat gcttgtgccg gatgtggcgt atccggcccg taaattcaat
    29221 agattacact tctaccgttg gcctgataaa catggcgcat caggcattac ggatcttttc
    29281 tttcgcccaa taaaaaacgc cccagtcatt actgactggg gcggctaaaa tattcagcca
    29341 aatccgatta cgtgaagtaa aaggtctgaa agatagaaca tcttacctct gtaccctacg
    29401 cgactaactt tactcttttt tgttcagttc agaaagcatt ttttgtaatt ttttttcagt
    29461 tgttgcatag gaatttctaa tagccatcac aaaacgtgac agcttatgtt gcttacttac
    29521 gaaaaaaggg catattcagg cgaatcttag tgcgcctgat cccagttttc gccactcccc
    29581 acttccacca gcaacggcac atccagacgg gtacagtttt ccatcagttg atgaatctgc
    29641 ttcgcgacgg catcgacatc atctttatga acttcaaata ccagttcatc gtgtacctgc
    29701 atgatcatac gtacacgcgg ttgctcagcc tgtagccacg catcaacggc aatcatcgcc
    29761 cgtttgataa tgtcggcggc ggttccctgc attggcgcgt taatggctgc acgttcagcc
    29821 gctgcacgac gagccccatt gctggatttg atatccggca gatacagacg gcgtccgtcc
    29881 agcgtttcaa cgtagccctg ctctttcgcc tgagcacggg tgcgttccat atactccagc
    29941 acgccagggt agcgttcgaa gtaaaggtcc atgtacttct gcgcttcttt acgtggaatg
    30001 ttcaattgcc gcgccagacc gaaagcactc atgccataaa tcagaccaaa gttgatcgct
    30061 ttcgcgctac ggcgttgctc gctggttacg gtttccagtg gcaaaccaaa cacttctgcc
    30121 gccgttgccc ggtggatatc ttttccttcc gcgaatgcgg tcagcaagcc tttgtcacgc
    30181 gagagatgcg ccataatgcg cagttcaatc tgcgagtagt ccgccgagac aatcacataa
    30241 tcctctggcg caataaacgc ctggcggata cgacgacctt cttcgttacg caccggaatg
    30301 ttttgcaggt taggatcggt tgacgataaa cgtcccgttg cagttactgc ctggtgataa
    30361 gaggtatgca cacgcccggt tttcgggttg atcatcagcg gcagcttgtc ggtgtaggtc
    30421 gatttcagct tcgccagacc acgatactcc agaatcactt ttggcaacgg atagtccagc
    30481 gccagttctt ccagtacctc ttccgacgtt gacggcgcgc cacccggcgt tttcttcagc
    30541 ggtttaattc cctgtttttc aaacagaatg gtttgcaact gcttggtgga ggaaaggtta
    30601 aattcttcac ctgcaatttc atgcgctttc ttttccagct cagccagacg aagagtgagc
    30661 tcttcagaat gattgtgcag cactttcgga tcgatcttca caccgttacg ttcaatgcgt
    30721 gaaagcaccg gcactagcgg catttcgata ttctcgaaga cgttcaacgg ccctttgtgt
    30781 ttttgcagat ccggccacat tttcagatgc agctgcaagg tgacatctgc atcttcggcg
    30841 gcgtaacgtc cggcctcttc gagggcaatc tggttaaagg tcagttgatt tttaccttta
    30901 ccagcaatct cttcaaaagt gatggtttta tgcttcaacc aacgttccgc gaggctgtcc
    30961 atatcgtggc gaccggcaac gctgttgaga atgtaggact ccagcatggt atcaaatgca
    31021 atcccccgca gttcaatgcc gtagttcgcc agaataccgc gatcgtattt caggttttgc
    31081 ccaaccttca gcgccttttc atcttccagc agcggtttta gcaactcgag tgcacgctcg
    31141 cgagagattt gatcgggcgc atcaagataa tcatgagcaa ccggaatata tgccgctacg
    31201 cctggctcga tagcaaaaga aagcccgacc aggttagcag agatgttatc aaggctgtcg
    31261 gtttcggtat caaatgcaaa taccggcgct ttttccagct tcgcaatcca cgctttcagt
    31321 gtttcttcat caaggatggt gacgtagttg tcataagaaa tcaccgttgc cgtcacttct
    31381 ggtgcttcgt ctgcaacact ggtttcctgt ggcttcgcgg ctggttttgc ccctttggcc
    31441 tgtaaccatt tgcccgcttc gacatcagca gtccagcgtt tgaactcata ctttttgaac
    31501 agccccaaca actcttccgc tgccggttgc tgcacttcca gttgttcgca ggtcagctcc
    31561 agttcaacgt cggttttaat cgtcgccagc tggtatgaga gataagcaac ttctttgttt
    31621 tgctcgagct tcgctgccat tgttttcgcg ccacggaagc tcaacccggc aattttttct
    31681 ggctcggcat acagcgtatc cagtccgcca agaccttgca gcaatgcctg tgcggttttt
    31741 tcaccgacgc ccggtacgcc tggaatgtta tcagaggagt cacccatcag cgccaggaag
    31801 tcgatgatca attctggcgg cacgccgtac ttattcacca cctcttccgg tccgaggatg
    31861 gtattcgtca tagtgttgat aagcgtaata tttggcgtca ccagctgcgc catatcttta
    31921 tcgccagtgc tgatcagcac cggacgcccg gctttttcgg cttcgcgcgc cagagtaccg
    31981 ataacgtcgt ccgcttctac gccagaaacc gccagcagcg gcagtcccat cgctttaacc
    32041 atcgcgtgca aaggttcgat ttgtgcacgc agatcgtccg gcattggcgg gcgatgtgat
    32101 ttgtaatgtt caaacagttc atcacgaaag gtttttccct tggcgtcaaa gaccaccgct
    32161 gcatgcgtcg gtttatattg catgatcaga ctgcgcagca tgttgaggac accatacatc
    32221 gcaccggtcg gctcgcctgc gctgttagtc agcgggggaa acgcgtgata tgcgcgataa
    32281 agataagatg aaccatctac aaggataagt ggattttggg ggatctgaac cataatgtcc
    32341 gtgcctgttt atcagattat gggtaaagga tgccacagac aggatgaaaa catgagtttt
    32401 tcaccgcttt tcgtgaaaat cttttgtaga tcttctggat cgctcgcaaa tcgtcatgtg
    32461 gataactttg tgcataaatt atttacatcg cttttaattt attatcatgc tttttacagc
    32521 atgatgtttg tctttgtttt tcatgaagtt aaaaataaga ccatccctaa taattgagat
    32581 aatgggattt tttctaatgt ggatagatat gaattatttt tctccttaag gatcatccgt
    32641 tatttgggtc gttttctgct aaaatctgcg ccttgcgctt ctttagcgca ctgtctatgg
    32701 ctaacttttt gaataaattc attatgacga gaatactcgc tgcgataacc cttttgctga
    32761 gtatcgtatt gaccattttg gtcactattt tttgctccgt cccgatcatc attgccggga
    32821 ttgtaaaact attgcttcct gtgccagtca tttggcgaaa ggtttcgcgt ttttgtgatt
    32881 ttatgatgta ttgctggtgc gaaggtctgg cggtattact gcatctgaac ccacacctgc
    32941 aatgggaagt tcacgggctg gaagggttaa gtaagaagaa ctggtatctg cttatttgta
    33001 accaccgtag ttgggcagat attgtcgtac tgtgcgtgct gtttcgtaag cacattccga
    33061 tgaataaata tttcctcaag cagcagctgg cctgggtgcc ttttcttggc ctggcgtgct
    33121 gggcgctgga tatgccgttt atgaagcgtt attcccgcgc ttatttatta cgtcatccgg
    33181 agcgacgtgg taaagatgtc gaaaccactc ggcgttcttg tgaaaagttt cgcctgcatc
    33241 ccaccaccat tgtcaatttc gttgaaggct cccgtttcac gcaagaaaaa catctgcaaa
    33301 cccactccac ttttcaaaac ttgttgccac caaaggcggc aggcattgcg atggcgctaa
    33361 atgtactggg taaacaattc gataaactgc ttaatgttac gctgtgttat ccggacaata
    33421 accgtcagcc gttcttcgat atgttaagcg gtaaactgac gcgaattgtc gtacatgtgg
    33481 atttgcagcc tatttccgat gagttacatg gcgactacat caatgacaaa agctttaagc
    33541 gccattttca gcaatggctg aactcactgt ggcaggaaaa agaccgactt ctgacgtcgt
    33601 tgatgtcatc acagcgtcag aataagtaag ttttactcgc ctctgaatga gcagaggcga
    33661 gtgagtattt taatgaacaa aacgtccggc acgagacata aattcttctt cgctcatttc
    33721 ctgtccgtta aaaacaactt tacccggtgt ataacgcagt tgcagcgagg cggtattgtt
    33781 gtcaatcgtg attaactgaa acatctgacc taatgtcatc atcccgctga tttgtttatc
    33841 agcccgcttt tgcgcttttt cagcatccat tccttcagat aaattaagct gttttgcggt
    33901 ttctgtgacg acattaagcg gtaacttcac atcaaaattg agcgatttaa tatctttgtt
    33961 tgtggatgat gaggatttgg ctgggtcggc aatactgata tccagattgg catttagttc
    34021 gccgagtgcg ttcttccatc ttaccggttg tttaatggtc ggctcacttt tttgtaataa
    34081 cggtaaatac tctttgaaca atgcagcatt cacttcttgc agagcaactt catcgtttgc
    34141 taactcaggg tgtgcagcaa gctgcttctg catcgcaatg ttatactgga tgataaattg
    34201 gcgcaccgct gaaggatcaa tagattcagc aatcaatgac cactcaccac taccgagttg
    34261 ctgattccct tttgttaact tatcaagttc gtaggtcaga ttgacattga tataatcttt
    34321 gtctttaacg cgatccagcc tggtttttgc aacgaaagta tcgatttggg caacgtcttc
    34381 cccgtgattg ataatgttaa tctgattaaa tttcgactcg ctttcaccaa ccggaaattt
    34441 cttttcttct aatcgcgcca gagaatcaat ggtaaagctt ttcgccgtta ctttgttcat
    34501 tggtgataat tgcagaacaa tatcttcgac tttgccttca atatccaggt tagacaatga
    34561 actaccatca ccattaaaag taaactggcc cttaccaaag gaaaactttc catactctgc
    34621 aggggcaaca tctaatgtcg tggagaatga gctaccaaac gcgaacctca atgtcgcttc
    34681 cacgggcgat ttatttttcg cggcgataaa tagcggttgt gtcagttcat tattcactaa
    34741 gttcactttt gccgctgcca gtgcggggat gacattacca cgcattaaca ttgtgatggg
    34801 caacggaccg tgctcaacat ccacatcaaa tgcaactttc tggcctggct tgatattgag
    34861 atcgggtgca ccgttatcaa atgttatttg catttgaaaa tgagaattaa aaaagccttt
    34921 gtcaaagttt ttataactta acgtcatatc gtaggcatgt tcacctttct ttttagcatc
    34981 gttaaaatct ttaatgaact tttcgacacc aggctgaatt tgcgtacctg tgtaccatgt
    35041 gccaccaccc cagattacgg caaacgcaac aataacacct gtagctgact tacgtatcat
    35101 ataaatattc cctgataaaa cagcaaaact
//
LOCUS       DACBVI010000036        34930 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.036, whole genome shotgun sequence.
ACCESSION   DACBVI010000036 DACBVI010000000
VERSION     DACBVI010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.036"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(200..448)
                     /locus_tag="IU325_08395"
     CDS             complement(200..448)
                     /locus_tag="IU325_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809051.1"
                     /translation="MFIYASGGNGGSAGGACANTSRLQGYVGGTLISVNASNNPAYGK
                     TAFISFAVPAGTSYQITSYPTENTSCGAGVFSVFGYQT"
     gene            complement(1153..1308)
                     /locus_tag="IU325_08400"
     CDS             complement(1153..1308)
                     /locus_tag="IU325_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon protein C'"
                     /protein_id="HAP5809052.1"
                     /translation="MNHFIGGKSGGSIYYKPIQCPTGYIMTGTRMYGIGDGVDEEHVD
                     AYCCPFN"
     gene            1436..2590
                     /locus_tag="IU325_08405"
     CDS             1436..2590
                     /locus_tag="IU325_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP5809053.1"
                     /translation="MPSPRIRKMSLSRALDKYLKTVSVHKKGHQQEFYRSNVIKRYPI
                     ALRNMDEITTVDIATYRDVRLAEINPRTGKPITGNTVRLELALLSSLFNIARVEWGTC
                     RTNPVELVRKPKVSSGRDRRLTSSEERRLSRYFREKNLMLYVIFHLALETAMRQGEIL
                     ALRWEHIDLRHGVAHLPETKNGHSRDVPLSRRARNFLQMMPVNLHGNVFDYTASGFKN
                     AWRIATQRLRIEDLHFHDLRHEAISRFFELGSLNVMEIAAISGHRSMNMLKRYTHLRA
                     WQLVSKLDARRRQTQKVAAWFVPYPAHITTIDEENGQKAHRIEIGDFDNLHVTATTKE
                     EAVHRASEVLLRTLAIAAQKGERVPSPGALPVNDPDYIMICPLNPGSTPL"
     gene            2741..3565
                     /locus_tag="IU325_08410"
     CDS             2741..3565
                     /locus_tag="IU325_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP5809054.1"
                     /translation="MRLNTTGIAARMMLSLDKKAIGEKLINGRQINPTPLQVRYPQGV
                     REVLGIMSEQLAISTADLTRILLEDALHNMFMPADNTTGNIISRIEYIMLSHDINAPL
                     LATLLSPWNIRSTVIQDPARLADYLSADALEHLAECFYLNPDWLNGHENYPIALSGEW
                     PDTADNFRMLISDSSNTEVIFWHSFPFAGNTKREYCGVILRQKKEINGSVIYPALSLS
                     PTLLNDEKRKWLTEYTTRQNTTMSLRRVTLRPGLAGNLITGQILPVSLFNTSLLPW"
     gene            3650..4852
                     /locus_tag="IU325_08415"
     CDS             3650..4852
                     /locus_tag="IU325_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HAP5809055.1"
                     /translation="MMKKNHITRTIIASAVLFSFNAAAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKHNSNDDFSLLLPSVGAQVADPDDVSNKADDVKDDWDLFDSAVD
                     NQHGVQQAAANLKHRLQEFRNINADAQVGVSAVAAMANDTLPFALMVKSYGTVSVNGK
                     VNDADLDYLDKVANGTITDVDKNALTSRAFGRAAVITDVGISFAKELETAGQKWSLGV
                     TPKYQRVDLFNYNVTVRDYDKDDFDGDKYHNTKNGFNADIGAYTDLNDNWTVGLVAQN
                     IIPRSIDTKVVNGFKETFKVRPQATAGVSWHNDLFTTALDIDLTPASGFTSDSKRQFA
                     SVGAEFNAWKWAQLRAGYRQNMASNSGSAFTAGVGISPFDVVHIDVSGLVGTDHDYGA
                     MAQLQFTF"
     gene            4912..5496
                     /locus_tag="IU325_08420"
     CDS             4912..5496
                     /locus_tag="IU325_08420"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase"
                     /protein_id="HAP5809056.1"
                     /translation="MMKPRSSYSKTAFILLFSVFLVAAVTKAKSSLPDITLEQAKEIN
                     ADNTVIFLFRHGERCDRSDMPCYSDKSGITITGTEKAQQEGIKFATIFSEYDIYSSNA
                     VRTIQTAKFFSGKEPVVMDSLSDCNNDLYKTLESIARESHKRNIVIMTHNHCLSFLAR
                     DRLGKKFKPAYLDALIMHYDGTRLILDGKYNKEA"
     gene            5891..6349
                     /gene="traH"
                     /locus_tag="IU325_08425"
     CDS             5891..6349
                     /gene="traH"
                     /locus_tag="IU325_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraH"
                     /protein_id="HAP5809057.1"
                     /translation="MNRIIPAGCLAALFLAGCNSPSQHTPVPRNYGAASDVAVTRHTQ
                     YTLWDKGVFSGQLPVSTGSHLLTANSDRLSFDWEGDAIELLNELARVRGMQFNYNGVR
                     LPLPVNLHVRDMTFSNTLRLIEAQTAWRATIHQYPGLLQVSFMQPENRKK"
     gene            6346..7164
                     /gene="traI"
                     /locus_tag="IU325_08430"
     CDS             6346..7164
                     /gene="traI"
                     /locus_tag="IU325_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraI"
                     /protein_id="HAP5809058.1"
                     /translation="MKALTSLLLPVILLTGCSSSPTISPGGVNTPPPDINAWLKPRSE
                     KPDSLSETRWKMLTDAGKTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISPEG
                     WLPPVIDEAQDVAHITPDQIRTSSRVWTIIRPERFVSNPPGWRDWLLRGLSTTATPGT
                     EGSVVPEDSVQRKVWETALRQGWQEGRQNADLTLEANQKTLTRDYRGMMLYSLLWRQG
                     MITRPDVSDQMQTVTGDGKKLVTGDRVRRLKNHAEFNLQKSHWRPLIGTEGGSR"
     gene            7161..8309
                     /gene="traJ"
                     /locus_tag="IU325_08435"
     CDS             7161..8309
                     /gene="traJ"
                     /locus_tag="IU325_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HAP5809059.1"
                     /translation="MNIPDEFGLFPFQRFTADELRHFFVWCAAHKVSDVDLTGGSPVS
                     VSRFGRRVRCSSATLPTTLMSSLIDELFGREVIPRVLAGNPVDRTIQINGDASGRYGL
                     KRGERIRLRSHLIQGTSGAEEKAISITMRVIPTEIPDILSMNIEPDLLEAMVCKSGLG
                     FVCGETGSGKSTLCSALYRYIMDNFPDAKIVTYEDPVEYILGNENDLLPPHQAEIGRD
                     VVSFAAGLRSAVRRNPEIIGVGEIRDNETADAAVQAGNTGHYCLSTMHTKSPGETLAR
                     LLGLFPPVIRDSMAWAVLSLLQFILVQVLVRTNDGGRKAVREYIVINDELRDNLSGMP
                     HAEWGHHIDAIIRQEKRRIRDQILEMYIRNEVDRREAILFIPPGELRS"
     gene            8306..8596
                     /locus_tag="IU325_08440"
     CDS             8306..8596
                     /locus_tag="IU325_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP5809060.1"
                     /translation="MKREGFWRYATPPVNIQGVPLPFTMIYLFICPFPSKTTFWICTG
                     IILFFVILDRSGWTVRTICTRIFSILRGAIASGRPWWYRHHTESPRDWTGLD"
     gene            8611..9162
                     /locus_tag="IU325_08445"
     CDS             8611..9162
                     /locus_tag="IU325_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1669 domain-containing protein"
                     /protein_id="HAP5809061.1"
                     /translation="MSGKKTIVTLLRVSLLACPLLFTTPSFAMIDTPSVKVGFSPEGS
                     ASALVLDTINSAESSIRMMAYSFTDPDVMHALAKAKKRGVDVRIVVDDKGNTNRASQE
                     AMKYINLLDIPLRTVDAFPIHHDKVIIVDGNTVETGSYNFSRAAARKNSENVVVLKNM
                     PDVAAQYLEHWQDRWNKGTDWKP"
     gene            9252..13019
                     /locus_tag="IU325_08450"
     CDS             9252..13019
                     /locus_tag="IU325_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP5809062.1"
                     /translation="MPSVSRYRTWLAVPADEIEDLKKAHPPMNGHTPVIWDKEHKLWF
                     ARPGADLSRLDRWLPRPQDVSMNGSDPVTEFAQVLENAGLVLKELPVMDGKIHRVPTA
                     DDKKGQKSGAYRGFLDGRPAGWYRDYRSADDSPITWTFSGGEQTDPRARLHLKAHSMQ
                     RREDAERELKAQYNRQAAYARRYVNKWPQATAHEYLTRKGIQAAPGVRVNNKNELVIP
                     FSNRNGAIRSYQRIPVTGGKDARILKDSEKTGNWFALGTPRNGQPVLFAEGYATAASL
                     HEATGLPVLMTVDGGNMIAVAENARQKWTQSPFIFCADNDHAIRVNKGIVSATKAAEL
                     TGGTVIFPAFTDAEKAQGLTDFNDLDASRGRAAFQHVINAQLEHIGVSTPNSNTPEIR
                     EALVIGNLVFTPVHTEEKTMTPTEYPETSPDTGHSHDQGPSLPSATQQEQPAASSSNI
                     ADETQSFTSHATENNGKDERHADNVQAQTESSVVQDSPAESPASPESTTASAPDEPAH
                     PAEPPEKVVSVATDKDWREFEAELSQPEKNESQQESGTSPEIPAPAAAPASPEDSPSS
                     ETMNESAEPSPSPDAVKEEMTVMTEPDDKQSTGISQGTPREEAVNHVTEDIPLQPEPF
                     LPESDGPEEYENYSAYQELMNNENPEHLQENSDMPSQPDTDHVQADESELQATTDTAE
                     PAVRDEPTPQQPVQSTSPSDNTSSFLDKARGFFTRKKTDSQAHENSDPTPSAETTTTP
                     PIPDSIVYAPERSDAPISLNMDEILKELDWEKRADRTVLYKLDGKPAFIDRVNRLEMV
                     NGASNDDRSVLAALAVATKFYGGVIELTGSDAFKQKAMQLIIEHNIDVRMKLPDQRAE
                     LEKLRKEMAVSKDAVVTHKPTPELNRNTPEQPAVPDPVQEKEATQSPASPVAPEASTV
                     STVPASSPAEPGKTADAAQGEEPPGKLRPGESVTAVLHNFGRAEYAPGKGESFFVELK
                     NRSGSKLYWGEQLESLVKNHQKGDVVTLTLQNREQFILPGEQKARFRNKWSMESVTNG
                     ISVSHDNPDKGQRIQAIPVETFMKVAAQISQGWPEEMKTLRMPENVGSHLFIGEDRHP
                     VSAPQNANQVTEITSAAPDKLTPVLGSVDKDTRELNLLLVQSADEHLQGVVRLNGTLY
                     PALATPSADNSQLVINALTDKGLRFAGYGEAVNHDADSTNRPAPELMQFHLKTREEPL
                     FAAVYTPEKQPDALYRNLGFEQSWQQWSNSQKPEDRQEKTLHQDLSHSPGR"
     gene            13037..13384
                     /locus_tag="IU325_08455"
     CDS             13037..13384
                     /locus_tag="IU325_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001629514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP5809063.1"
                     /translation="MNMHKSLVAVLGLILISSTASAATCRAGSAAQAGSNTGYERARA
                     AADAWSQRENDVSSSLQSCLSRIKKISINLPQFPSLDDILSQLENQVCDAVVDKVNEK
                     LPGNIDPWKDYNL"
     gene            13381..14073
                     /locus_tag="IU325_08460"
     CDS             13381..14073
                     /locus_tag="IU325_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraM"
                     /protein_id="HAP5809064.1"
                     /translation="MTENTEPSSPSHPAPDPEILKSTIRAMKQSEQRANNVPALIKAL
                     LCTGTCLLISITGNAIQYWHSTNVEREYFATDNGRLVRLAPTNQPAWSQNDAMAFGSQ
                     ALATAFNLDFVHYRSQISSLSPRFSDEGFAGYVNALQASNILETIKKEKMNLTATTGA
                     GVLVRQGQMSDGVWFWTFQYPVRMRLVGQTTSKPEQSFVFEITIQRVDPRLKPSGMEI
                     RQMISRNAGPNS"
     gene            14084..15067
                     /gene="traN"
                     /locus_tag="IU325_08465"
     CDS             14084..15067
                     /gene="traN"
                     /locus_tag="IU325_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraN"
                     /protein_id="HAP5809065.1"
                     /translation="MQSRYLLSTLLLVCSAATSADNAGWQNARTPQTTNSASDHVQNA
                     SQNTGGVPATLVKGELPAPGQASPLVQDAARLDSELSADEIRSLRSLMADNERAINAP
                     ITSVVPRISSLTVNLSPGASLPLVRTAMNNLSVVTFTDINGSPWPQSDPPYNAAPKLF
                     DVQYNENMVTITPLRPWASGNISVYLKGLSVPVILNVTSGETDTPSSSQEMDSRLDLR
                     IPRQGPTSPVVSIPTDKIALHDATLQAFLDGIPPRDPSVKRLKFTGNVPDTTIWQHGD
                     DLLVRSRAMLRDEFEQTLSSADGTHLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            15070..16359
                     /gene="traO"
                     /locus_tag="IU325_08470"
     CDS             15070..16359
                     /gene="traO"
                     /locus_tag="IU325_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HAP5809066.1"
                     /translation="MSAEQDAGKSGKKLAALLGLGSIILFGVGYIAFSKLSGSNSDMQ
                     SAVNINSAASGGTRSVTETPHYRELLRADNERGAAAAARNNQTFIASLPQGLDIPDTP
                     EKQQQPAAKPENYAHRQASGTPQEDRAASEKRMERLQKLIVRIKDQHPAGSTPTIATA
                     MWNKSPAETTGQNGTQQFALQNASLSTPVAEKGIQLIPALTRIPAYIDTAVDSDNPSS
                     KVIATIPAGPWAGATLFSPGVKLVGNGVEIHFDRMSWNGMDLKVNAYAQREDNLMSSV
                     ASNVNTRWFKHIILPSVLGGVGSIGTLYKDANTQVIQGNYGTVTGRVGMPSGEAVAGV
                     IAGGMAERGSQILTRQAESEPYKQVEVYQHEVVSILFVDPVMTNDARSSSLSSGISPS
                     VNRTSQTEQRSQARMQTAMEQRKAVMQRRYDEQPETP"
     gene            16359..17063
                     /gene="traP"
                     /locus_tag="IU325_08475"
     CDS             16359..17063
                     /gene="traP"
                     /locus_tag="IU325_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraP"
                     /protein_id="HAP5809067.1"
                     /translation="MKPETEIDETGSFGEPEEKPAPFWKRSVWGISVATWGLCAVVLL
                     AAIWYLFLRAPSETGMPPFNDADAGVQTWQTTQESSPSVQSTETMTVRTGEDMSQLAR
                     DVKTELDNRDEKIQATLNMLHDSINKLGEAIKKDEEYAQETRRQLDDIRSHLNGMMTQ
                     KSVTESSSTPHPAKKKTSSVLNGMKIMSMETGMAWIRWQGSTWAVREGQTLGNVVIQR
                     IDPTTRTIITSAGTLR"
     gene            17063..17590
                     /gene="traQ"
                     /locus_tag="IU325_08480"
     CDS             17063..17590
                     /gene="traQ"
                     /locus_tag="IU325_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HAP5809068.1"
                     /translation="MNMDALTAIENFASSIFSAGMDFLFTWGEFIGVISMITLFARAR
                     SAGPVKMSPGKFIAGMLTSCMLVSLPAMINAGGVQMGFRADSFGPIAYVQPQTFGAAA
                     GAANAVLSLAKLAGVGFVMNGISIWRKAGLDGHTALSASESVSKGNVKFIAGVLLVFI
                     DRVLNALLASIGIVF"
     gene            17641..18045
                     /gene="traR"
                     /locus_tag="IU325_08485"
     CDS             17641..18045
                     /gene="traR"
                     /locus_tag="IU325_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraR"
                     /protein_id="HAP5809069.1"
                     /translation="MSRIRVVAARLYAAFIHAQLFVMSCRSKLTKFLLLIPMMLLPKA
                     VLADGDLADMVRNVEQGAKTAQSSSLTIAQFIGVILFLGGLIGLKKVGKQGGMGLAGC
                     IVSIVIGALLVAGPEMMSRSQKQLGISTISIG"
     gene            18109..18297
                     /gene="traS"
                     /locus_tag="IU325_08490"
     CDS             18109..18297
                     /gene="traS"
                     /locus_tag="IU325_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative conjugal transfer protein TraS"
                     /protein_id="HAP5809070.1"
                     /translation="MSAWSPEHITLRRTENGNVIAHDSRDDQEFIFAECCSDEIANII
                     LEAVKRYTSTEPAHATQH"
     gene            18281..19081
                     /gene="traT"
                     /locus_tag="IU325_08495"
     CDS             18281..19081
                     /gene="traT"
                     /locus_tag="IU325_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraT"
                     /protein_id="HAP5809071.1"
                     /translation="MQHSIKDLWLYPFPEIDVVHTQEPLLPEPELTTPGRCICCRQNV
                     RHRFRLDDSWPLRQLTDTISDTRVRLNKATEHLDKLKKRGEPVATGEKEKYNTAVKAA
                     ERALEQARLSARRLSLRHVQKAEITSTESLSEKEQELFHEDGPPYSLCAFCHAWHSLN
                     GYTAAQGVMVWLPDLHPSTVVALNRRSLQEVFSNDKFRVRRGREALSALMQNRLAVED
                     KFRSFRPADFADVFRRYPPSGRSPLREKMNGIALILTPDSFIKKEYVD"
     gene            19171..22215
                     /gene="traU"
                     /locus_tag="IU325_08500"
     CDS             19171..22215
                     /gene="traU"
                     /locus_tag="IU325_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraU"
                     /protein_id="HAP5809072.1"
                     /translation="MNINRLIFTIEDCLNTLSRFSVASSFVEYCDLRTVIGLDRQDRE
                     RRPWLNSPYIAATKRGEYLSVFEVSGAFREMDEASDQTGPGSLESLITSMSDSLNTAY
                     KNSGHKISFVFERDPDMGKEEIEDMVAPQKRSLANTGIQLQDVVDEKVTTLSPWLVRE
                     RCWLAIWSGPDLISNSDRTAHDELVRRLAERVPKARFAQSPWQWTLSALKIRHEAFLD
                     NVEQALRHSSDGLILRLLDIHEVGREIRRQTERHSTPRNWQPHLPEDAQPAGYRWTDD
                     ESVLHAPSLHLQLFNTQVTTQGNLVQAGGLWHGMVSITLPPQNLQTFNELVRAVPRAV
                     PWRIRMDLMPGGMKALNLKKTLLTYSSFISAVRPMYESVMTLAATDEKEPVCIMTIMA
                     STWGKTREICTRNQAILKSAIEGWGVCGTTTTFGDPRRAWVNTILAASGGSGPVPLYP
                     PLSHAISLFPLNRAGSVWRGKGNLMLHTEDGSAFEVGLASSQQNKHTELAPGDPGLGK
                     SVLINTLSEIQISSAQKNLPFIAYIDKGYSAQGLVQLIRDSLPQERKDEAVGIILSND
                     PDYTRNLFDVMYGAKKPITPEKNFMSSVLCALCVDTGTGQPCNPGDTRQIINQLIELA
                     FKEYGENNPRLYRASTEELVDSALQDSGLYEKHDAAWWARSTWFEVRDMLHNAGYIMA
                     AQRAHYQAMPQLPEVSSMLGHTSLRDVFGTVQRDGSNELLLDYIRRALEQGHNDYPMI
                     SGYTRFMINPETRVIAVDLNNVAGDKTPAGRLKTGIMYLLAGQIAGGDFTLPQYRDEV
                     LKQLPREYHEIALKRINQLDQEVKTKVYDELHNARGIDFIWENLDTQEREQRKFAIRT
                     VLSTQYLRDYPESVLKSANTLWLLRYKPEDIPVLRDNFNVPEFMLKRFLKMPEGPAPD
                     GSGVPVLGVFRVKSGTLARILKFTVGPLELWALNSSPKDSALRKTLTNKLGSVRARKI
                     LAENFPRGSATSLIEHRAGQHNSDNVIEELASELIRKQGYNL"
     gene            22215..22829
                     /gene="traV"
                     /locus_tag="IU325_08505"
     CDS             22215..22829
                     /gene="traV"
                     /locus_tag="IU325_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraV"
                     /protein_id="HAP5809073.1"
                     /translation="MCTIHITPRCRRPARGQVFLKKLEVKGDSFSFTLPSRVFRLHPA
                     PNPRVIYASDARTQGKVTGMRLLLPSSTDELITTAIWQVSVPDEAPFDVQHVIQWILP
                     SPLASLMSDDTWLWPSVPGTPALSAGDWPVMDTDPSTLRRVRRRSFSIIDNRNRKGYI
                     TRRFQCFPLAPLPENRKYDSLPDLVLHPEQETIKCKEKHYSQPL"
     gene            22796..23998
                     /gene="traW"
                     /locus_tag="IU325_08510"
     CDS             22796..23998
                     /gene="traW"
                     /locus_tag="IU325_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraW"
                     /protein_id="HAP5809074.1"
                     /translation="MQRKTLLAALIATLSGTACQAHAYSVTVVASRPVEEQVIPRMEA
                     IKDVLGNILSTQTATGTAINQNSEKLASVIAQNGQATRQQMIFSNETQRLEEARKSFT
                     VPDSICSESASGIATESKSASASAASKLSKGGGVSNRSIRDRLASAANSPVREAYDGA
                     AIHASYCTEAEYARFGGTAVCPSVGEIPGGDSQVRSIYHGAGTADTPAALTWDQKQID
                     AATAYMKNTSRPSAGRALGKGEVNTQSGRTYVGLQNEYNGIIDSASNPQLTLIADSTP
                     NESTRKALAETLQSDSAAAYFDQVASPEAKARGYMSTREFEAFEAGRRYANTAYLVDL
                     QEMQGDNLLRELVRITAQMNWQLNDLKEQIRQGNVISGQQLALTARQYYEKQLGSLEK
                     TINQANAR"
     gene            24027..24611
                     /gene="traX"
                     /locus_tag="IU325_08515"
     CDS             24027..24611
                     /gene="traX"
                     /locus_tag="IU325_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraX"
                     /protein_id="HAP5809075.1"
                     /translation="MNKLPENSDQTKNVKKSPSITRIATRGIINIFVPLMETRLITNS
                     VQHMLEQQKRRLDRFRQVNNKKAQLHLTWEEALQASHMSVDDLDRRFRRRRTVWRFCC
                     WSLLAIALFLSGMLFAASSLPLTTLVRAISTLVLILSGVALCASRALIVTYRLWQLHE
                     RKVSEPEQGTFRNFLNDRNGWRNATLIAVTSKQY"
     gene            24708..26876
                     /locus_tag="IU325_08520"
     CDS             24708..26876
                     /locus_tag="IU325_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HAP5809076.1"
                     /translation="MKILLRALCAGLAISSLPAMASVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLSTSAPTLIGSMFGAFNSIIAVLAVVWFMFIGIRHVVRSGHQGQVFST
                     GRDIVGTLSVVAGFLMIVPTGNGWSIAQLIMLWGASIMGVGSANVMVQLAADNIANGY
                     SMTVQPVQASTRTAARGIFEMELCKYAVNAGLNDFNQTVKSSTSLMTESAKTASGNYT
                     VTVSNGSGICGSASLSVEGNGTTDQSTIGKFFNPFSKNEYSGVISAQRAAMDNMISDM
                     DNAASEFVTTFLEKRNSGNGTLPDIETRIQRAADEYERAVQKSLPIDNGEQSRKEALK
                     SYLTTYGWVTLGAWYQTFATANQRLAELADRAPAVTSMSSLGEVGDTDLFSAVMSAYR
                     TQLQNSSYTPPLGTVVSSSEQRMANAQDPKSALSEIMGPLVSLTNRIATETSGTGTTS
                     AQINPLIKMKNIGDITMVSAEGIWTIYTTARVVVAGGKDSILVKFFNSLTGAASMLTA
                     LFEALAPPVYFLLFLMFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCTAGPLWAA
                     THLGTSEDRGSRAAYGYIYLIDSMIRPPIMVFGFFFASVAIIAVGTILNALFGAALVN
                     VQFNSLTGIFSLAGFLLIYARICTTTVAAIFALQAYLPDHVINFLGGRDGANTLGSMA
                     NSVKEIFIGGSRNIRHTPGMKTDRLKDNTKGSDDKDGIKG"
     gene            26947..27609
                     /gene="excA"
                     /locus_tag="IU325_08525"
     CDS             26947..27609
                     /gene="excA"
                     /locus_tag="IU325_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HAP5809077.1"
                     /translation="MKAKRETTDRFPTWWLFYYVLRKAYFFLGIPFFLFCALGFTEML
                     CSDRYFGNKVEDYVVTFGSWFLLLAPGIWMYSRAKTRREKIRKVVQTIKESGFYSPEK
                     GYEGLSLTQGAYFGIDLKNGTMLYVRIYPGNIMDVIGFDIHNFTRTVTDDKTLEIHTK
                     YINLPMVPIPSWCTHPETASNTMHAMASRGYDYPVDFPRLIQEKRKEWEQIAGVPVAE
                     VF"
     gene            complement(27681..27890)
                     /locus_tag="IU325_08530"
     CDS             complement(27681..27890)
                     /locus_tag="IU325_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HAP5809078.1"
                     /translation="MSMPPAIANTFLFEMMKSKSKDITLAAIYALGEGRCQADNIISE
                     LERLSQSDDMEIKIAAIKALGRIYR"
     gene            29057..29308
                     /locus_tag="IU325_08535"
     CDS             29057..29308
                     /locus_tag="IU325_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809079.1"
                     /translation="MSEKWSKEFDSCFQVGSIEEIADALEKRSGSEENALAMLAMTAF
                     TLMTRRGICEMQNVAPDGKGIRLELIGFRENETIPDTLH"
     gene            complement(29380..29532)
                     /locus_tag="IU325_08540"
     CDS             complement(29380..29532)
                     /locus_tag="IU325_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HAP5809080.1"
                     /translation="MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATL
                     AYEVKR"
     gene            29824..31032
                     /gene="trbA"
                     /locus_tag="IU325_08545"
     CDS             29824..31032
                     /gene="trbA"
                     /locus_tag="IU325_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HAP5809081.1"
                     /translation="MSYNRQPVAEDPMQIWGAVGVLLILLLFVIWLFLPEVVYASCLI
                     LHTLWGLVDWGPFHNYAAPRYNLLAMTGNNAANISYSQWVNVMEQTIGILWMYLLPVT
                     LWCLWEWYQHPGQSRFTRRPVDITRLPHIFASLSPAIAPVLADGDPEKLFHGGKRPER
                     RVALTPEAFVEQHTLITNMQLDVAAARRCFMAQLGKPLTSWKDMAPHEKALFAIFGLQ
                     YFLDDRKAALKLMDTLNLSCRIKSKRDSGKFCTPVYSLAKSAFQRVIKSNEAQQWLKQ
                     HRYVRSGLVWLYAHDLRLTPPNWIWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     EAEAMRFGLPCPEPCVDEAVEGLRRDMLSLGLIWDEPQPDRDRKRRILTNWSLTDDIL
                     PRTPATDNEF"
     gene            31051..32121
                     /gene="trbB"
                     /locus_tag="IU325_08550"
     CDS             31051..32121
                     /gene="trbB"
                     /locus_tag="IU325_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbB"
                     /protein_id="HAP5809082.1"
                     /translation="MTIEYFATRIAKHISVQAIWPNGRTEIIAVLPRDALSGLVTRNN
                     QLIHIAFDAMGTRNRETVLIDKQEHNADQILTAIDSCLRLEYLMERRFSRTPLFRGII
                     ASVVLFVMATIGFSLFRYVDRVFWDDTTPEAVQTAGEPRLLPPHLNHTVPLNEGIQLP
                     VPPKKDVQVPEKTITSKNPEAAAARHNLAAVLKRNADRGMFTINLSSGHERTLYAFLD
                     PACPNCRLLEPALKRLASDFNVVIYPVSVIGGEESTDRVAPLLCEKDAQKRAAGWHRL
                     YSADNGMMTPSEETTPADETCLKAARAAIDVNNVAFRKFGFAGTPWVLSDTGWHLPTG
                     ILQETGTLNLFLKTTDSESGHE"
     gene            32114..34405
                     /locus_tag="IU325_08555"
     CDS             32114..34405
                     /locus_tag="IU325_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HAP5809083.1"
                     /translation="MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIW
                     PLALPACLLFTLITMLVFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQY
                     EVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINP
                     LCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTR
                     PQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTS
                     AHTTSALGRMFITQKSMILARDLGYRLEGTDSDALEVKKYKGRFPYLCFLDEVGAYYT
                     DRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLMQNTGTKFAGRIVSEGSTA
                     RTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELHPGEFFS
                     IFRGETVPSASFFIPDDEKSCSSDPIVINRYISVDAPRLDRLRRLVPRTAQRRIPSPE
                     NVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESA
                     LWETAVNTLKTTTREERRIRYITLNRPELPETNEENQISVRAERAGINLLTLPQDNNH
                     PTGRPVNGFHHKKNNRPDWDGMY"
BASE COUNT         9524 a         8834 c         8357 g         8215 t
ORIGIN      
        1 ggacaatagg tggaaaactc aaagttactc agctttccac cacaggttat ctggggcaat
       61 tcgacttctg tgccattgcc agaatgggca acgcagagga tgcccactac tgccaggtag
      121 ttgagagccc agcaggttca cgcaaatggt acaaatacga gcataagaca ggttgtattg
      181 cgtcgtgtgt aacgctcaat taagtttgat atccaaatac tgaaaatacc ccggcaccac
      241 atgatgtatt ttctgttgga taggatgtta tctgatagga agtacctgca ggtacagcaa
      301 agctgataaa ggctgttttt ccatatgcag ggttattgct ggcattcacg ctaattagcg
      361 tcccaccaac atatccctgc agtcgggatg tatttgcaca tgcacctccg gcagatcctc
      421 cattacctcc agatgcataa ataaacaatg tactgccccc tgaattccgc cctgagaatg
      481 aacctctatg tgaacctaag tttgtgtaag aaccattgag cgaaccagaa gtcttccacg
      541 taccggattg gcactggcct acgtgccaat ccggtacttg gcgtaaaagc aattctggta
      601 gtacggtgat aactggtcgt atagcaaatg gtcagcaaat accgctgcca acaggatttt
      661 ctgccagtca gtgctcatgg agtgtgagca atgcagaaaa ccctcaaggt tggaagccga
      721 attactttgc aggatcagtt gcaacttatg atgcaaatcg aattgttaaa tgtggttttt
      781 atgatgaata caatttccat aaagggacat ttagagctga tttaaccgga aaatgtagtt
      841 atgtcgtcgc gtgtcagaac tgagattaaa ttgctggcgc tgctggccta cgtgccaatc
      901 cggtacctgg ggcgctccta aaattcaatt cacaacgcag acctacaaca tagctaaaaa
      961 cactcgtaat ttaaggctcg gggtccatgc atattgctca tggacttacc tgaatggctc
     1021 accctttggt ggttttcaac aggtatattc cgaccaaaac aacgtttggt atgtgagtaa
     1081 ttatgcttgg ggaaattatg agtctggtgg gaccatatca gtcacatgcc tcaatcttcc
     1141 tggtgctgga gcttaattaa aggggcaaca gtaggcatcg acatgttcct catcaacacc
     1201 atcaccaata ccgtacatgc gtgttccagt cataatgtat ccagtcggac attgaatcgg
     1261 cttgtaatag atcgaaccac cactcttacc gccgataaag tgattcattg ctacggatga
     1321 tttgtaccaa ttacatgctg agtaattaat tttattaccc cctgaccacc gaccggattg
     1381 gcacgcttgc agagaatgat tgttggcatt attttttgcc tcttttgagg ttagcatgcc
     1441 gtctccacgc atccgtaaaa tgtccctgtc acgcgcactg gataagtacc tgaaaacagt
     1501 ttctgttcac aagaaagggc atcaacagga gttttaccgg agcaatgtta tcaagcgata
     1561 tcccattgcc cttcggaata tggacgaaat aacaaccgtt gatattgcta catacagaga
     1621 cgttcgttta gcagaaatca acccccgaac gggtaaaccc attacaggta atactgtacg
     1681 tcttgaactc gcccttctgt catctctgtt caatattgct cgtgttgaat ggggaacctg
     1741 tcgtactaac ccggttgaac tggttcgcaa gccgaaagta tcctcgggac gagatcgccg
     1801 gctaacgtct tcagaagaac gtcgcctttc tcgctatttc cgcgaaaaaa atctgatgtt
     1861 gtatgtcatt ttccatcttg cccttgagac agccatgcgg cagggcgaaa tactggcctt
     1921 acgttgggag cacattgatt tgcgccacgg tgtggctcat ttacctgaaa ccaaaaacgg
     1981 tcactcacgg gatgttcctc tgtccagacg tgcccgtaac tttcttcaga tgatgcccgt
     2041 taatctccac ggcaatgttt ttgattacac cgcatccggc tttaaaaatg cctggagaat
     2101 agccacacaa cgacttcgca tcgaggacct gcattttcac gatctacggc atgaagcaat
     2161 aagccgcttc ttcgaactgg gtagcctgaa tgtaatggag attgctgcaa tatcaggaca
     2221 tcgttccatg aatatgctga aacggtatac tcatcttcgt gcatggcaac tggtcagtaa
     2281 gcttgatgcc cgccggcggc agacacaaaa agtggcagca tggtttgtgc cgtatcctgc
     2341 ccatatcacg accatcgatg aagaaaatgg gcagaaagcg catcgtattg agatcggtga
     2401 ttttgataac cttcacgtca ctgccacaac gaaagaggaa gcagttcacc gcgccagtga
     2461 ggttttgttg cgtacactgg ccattgcagc acagaaaggc gaacgtgtcc catctcccgg
     2521 agcgttacct gttaacgacc ctgactacat tatgatttgc cctctgaacc cgggcagcac
     2581 cccgctgtaa acatcattca aaacacatac agaaaggcga gttgcaacaa ctcgccaaac
     2641 acattcgatc tgcatgatcg aaattattcg tttcaccaga aaaaacaccg aagatacaat
     2701 aaccacatga gctgaaaaga ctttttcagt cggaggaaca atgagactga atacgaccgg
     2761 tattgcggcc cgaatgatgc tctcgctgga caaaaaagca atcggcgaaa aactcatcaa
     2821 cggtcgtcag ataaacccga cacctctgca ggtccgttac ccccaagggg taagagaagt
     2881 tctggggata atgagcgaac aattagccat atccaccgct gatttaaccc gaatcctgct
     2941 tgaggatgct cttcataata tgtttatgcc tgcggataac accacaggaa acataataag
     3001 cagaattgaa tacatcatgc tgtcgcatga tataaatgcg ccattactgg caacattgct
     3061 gtcgccatgg aatatccgct ccacggttat acaggacccg gccagacttg cagactatct
     3121 ttcagctgac gctctcgaac atcttgctga atgtttttat ctcaatccgg actggctgaa
     3181 tggccatgaa aactatccga tagcgttgtc cggtgaatgg ccagatactg ctgataattt
     3241 cagaatgctt attagtgata gcagcaacac tgaagttatt ttctggcata gtttcccctt
     3301 cgccggaaac acgaaaagag aatattgcgg ggtaatattg agacagaaaa aagaaataaa
     3361 tggctctgta atatatccgg cactatcatt atcaccaaca cttctgaacg atgaaaaaag
     3421 aaaatggtta actgaatata caaccaggca gaatacaaca atgtctttgc gacgcgtcac
     3481 gttgagacct ggccttgccg ggaaccttat tacagggcaa atactacctg ttagcttatt
     3541 taatacgtct ttattaccct ggtaaatacc agatagtacc aaagtaaaaa ttacggcaca
     3601 tccgtgtcat agcgttgttt tgtctaaatt ctgataaaca ggaaattaaa tgatgaagaa
     3661 aaatcatatt acccgcacca tcattgcatc tgctgtatta ttttccttta acgctgcagc
     3721 agcgacgtca tattttgaag cgcgtaacga tgcaatgggt ggtaccgggg tagcatcttc
     3781 ccattatggt gtggcacctc tggcaaaccc ggctttactg acaaagcaca acagcaatga
     3841 tgatttcagc cttcttctgc cgtctgttgg ggcacaagtt gctgatcctg atgatgtatc
     3901 aaataaagct gatgatgtta aagatgactg ggatttgttt gacagtgctg tagataacca
     3961 gcatggcgta caacaggcag cggcaaatct taaacatcgt ctgcaggaat tccgcaatat
     4021 caatgcagat gctcaggtag gtgtatcagc tgtagcagcc atggcaaatg acactctgcc
     4081 gtttgccctt atggtcaagt cttacggaac tgtcagcgta aacggaaaag taaacgatgc
     4141 agacctggat tatctggaca aagttgccaa tggaacaatt accgacgtcg ataagaatgc
     4201 cctgacctca cgcgcattcg ggcgtgcagc tgtaattacc gacgtcggaa tttcatttgc
     4261 caaagaactg gaaacggccg gacaaaaatg gtctcttggc gtcacaccaa aatatcagcg
     4321 tgtggacctg tttaactaca acgtaacggt tcgtgattac gacaaagacg attttgacgg
     4381 tgacaagtac cacaacacga aaaacggatt caacgccgat attggtgctt atacagacct
     4441 gaatgacaac tggactgtcg gtcttgttgc ccagaacatc attccacgca gtattgatac
     4501 taaagtcgtc aatggcttta aagaaacctt caaagttcgt ccgcaggcaa ccgccggtgt
     4561 atcctggcat aatgacctgt ttaccactgc tcttgacatt gatctgactc ctgccagtgg
     4621 cttcacttct gacagcaaac gtcagtttgc aagtgttggt gctgaattta acgcatggaa
     4681 atgggcgcaa ttgcgtgccg gttatcgtca gaacatggca tcaaactccg gaagcgcctt
     4741 caccgctggc gttggtatct ctccatttga cgtcgtacat atcgatgttt ccggcctggt
     4801 aggaactgac cacgattacg gtgcaatggc acaacttcag ttcactttct gattactgtc
     4861 cccgcccccg gcactaaacc cgggggtatt tttatattca gtttaaataa catgatgaaa
     4921 ccacgttctt cttattcaaa aacagcattc attcttttat tttctgtttt cctggtagcg
     4981 gcagtgacaa aagcaaaaag ctcgcttccc gatatcaccc tggaacaggc aaaagaaatt
     5041 aatgctgata acaccgttat tttccttttc cgccatggag aacgttgcga ccgttcagat
     5101 atgccatgct attcggataa aagcggaatc actattaccg gtacagagaa agcacagcag
     5161 gaaggtatta agttcgcaac aatcttcagt gaatatgata tttactccag caatgcggta
     5221 agaaccatac aaactgcaaa attcttctcc ggtaaagaac ccgtcgttat ggacagttta
     5281 tcagactgca ataatgatct ttataagacg cttgaatcaa tcgcaagaga atctcataaa
     5341 aggaacatag taataatgac acataatcat tgtttatcat ttctggccag agataggctc
     5401 ggcaagaaat tcaaaccggc gtaccttgat gccctgatca tgcattacga tggtacccgg
     5461 ctcatacttg acggaaaata caacaaagag gcatagagga tatttgaatt tacataaatt
     5521 ctgatatact cagtaagata caagagtgta cgggcacatg tgcctgtctg aattgcaggt
     5581 tatccggacg agggccggga actgtttcag ggacaagcgc cggtacgttc gattcataaa
     5641 tcgtcgagtc atccatcatg gatcgattcg gcgcggagcg ttaaagatta cgctcatatt
     5701 cgttttatgt ctgacgacac atcaaccagc cagtatcagg aataatcacc ttcagtaaac
     5761 tatgaggcta tttacctggc cacactggca tttatttccg gtaacgcaag tatagccgga
     5821 aacaaacatc atcctgtctg taccggagca ccggtatatc ccattctgat ttaactgctc
     5881 aggaattatt atgaaccgta ttatacctgc ggggtgtctt gccgcccttt ttctggctgg
     5941 ctgcaactcc ccttctcagc atactcctgt tccgcgtaac tacggtgctg cctctgatgt
     6001 ggcggttacc cggcacactc agtacacatt atgggacaaa ggtgtatttt ccggccaact
     6061 tccggtttca acggggtctc atcttctgac agctaacagc gatcgccttt cctttgactg
     6121 ggaaggtgat gcaattgaat tactgaacga actggccaga gtcaggggga tgcagtttaa
     6181 ctacaatggt gtccgcctgc cgttaccagt caaccttcac gtccgggata tgacgttttc
     6241 caatacgttg agacttattg aagcccagac agcctggaga gcaacaatcc atcaatatcc
     6301 gggcttgtta caggtcagct tcatgcagcc ggagaaccgg aaaaaatgaa agcactcacc
     6361 tctctccttt tgccagtaat actgcttact ggttgttctt cttcccctac catctcgcca
     6421 ggtggtgtca atacgccacc acctgacatt aatgcatggc ttaaaccacg aagtgaaaag
     6481 ccggatagtc tgtcagaaac acgctggaaa atgctgaccg acgcaggtaa aacgcttgga
     6541 tttcgtggtg gcaaggctca gcgttcatgg gagctgattc aggcgctcaa cgcccgggaa
     6601 agcacactta atgccttata cgatttccgg ccactgatta gcccggaagg ctggctacca
     6661 ccggttattg atgaagcaca ggacgtagct catatcacgc cagatcaaat ccggacctct
     6721 tcccgggtct ggaccatcat tcgccctgaa cgctttgtca gcaatccacc gggctggcgt
     6781 gactggcttc tccgggggct ttcaacaaca gccactccgg gtacagaagg cagcgtcgtt
     6841 ccagaagaca gcgtgcaacg aaaagtgtgg gaaaccgcat tacggcaggg atggcaggaa
     6901 ggaagacaaa acgccgacct gacgcttgaa gccaaccaga aaaccctcac ccgtgattac
     6961 cggggcatga tgctgtactc acttctctgg cgtcagggca tgatcacccg tccggatgtt
     7021 tctgatcaaa tgcaaaccgt tacaggtgac gggaaaaaac tggtcactgg agatcgtgtt
     7081 cgtcgcctta aaaatcatgc ggaattcaat cttcagaaat ctcactggcg tccgcttata
     7141 ggcactgaag gaggctcccg atgaatattc ctgatgagtt tggtcttttt ccgtttcagc
     7201 gtttcactgc agatgaacta cggcatttct ttgtctggtg tgccgcacat aaagtcagtg
     7261 atgtggatct gaccggcggg tcacctgtat ctgtcagtcg ctttggccga cgggtgcgtt
     7321 gttccagtgc aacgttacca accacactca tgtcctccct gattgatgaa ctgttcggtc
     7381 gggaagtcat tccccgcgtt ctggcaggca atccggttga caggacgata cagattaacg
     7441 gggatgcatc cgggcgctat ggtcttaaac gcggagaacg catccggtta cgctctcacc
     7501 tcattcaggg cacatctggt gcggaggaga aagcaatctc aataaccatg cgtgtcatcc
     7561 cgacagaaat accggacatt ctctccatga acattgaacc agacctgctt gaagcaatgg
     7621 tctgcaaaag cggtctggga tttgtatgtg gtgaaaccgg gtcaggaaaa tcaacgctgt
     7681 gttcagcact gtaccgttac attatggaca atttccccga cgccaaaatc gtgacatatg
     7741 aagacccggt ggaatatatc ctgggaaatg aaaacgatct gctcccaccg catcaggcag
     7801 aaatcgggcg tgatgtggtc agttttgctg ccggactgcg ttctgctgta cgacgtaatc
     7861 ccgaaattat cggtgttggt gagatcaggg ataacgaaac cgccgacgca gcggtacagg
     7921 caggcaacac cgggcattac tgcctgagca ccatgcatac gaaatctcct ggtgaaacgc
     7981 tggcacgtct tcttgggctg tttccccctg tcatcaggga ttcgatggcc tgggctgtcc
     8041 tgtcactgtt gcagttcatt ctggttcagg ttctggtcag aacaaatgac ggtggtcgta
     8101 aagccgtccg ggaatatatc gtcattaacg atgagcttcg cgacaatctt tccggaatgc
     8161 cgcatgcaga gtgggggcat cacattgatg ccataatccg gcaggagaag cgccgtatcc
     8221 gggaccagat tctggaaatg tatatccgga atgaagttga ccgaagagaa gcgattcttt
     8281 ttatcccgcc aggggagtta cggtcatgaa aagagaaggt ttctggcgat atgcaacacc
     8341 gccagtcaac atccagggcg tacctctgcc gttcaccatg atttatctgt ttatatgccc
     8401 ctttccctcc aaaacaacct tctggatttg caccggcatc attttatttt tcgttattct
     8461 cgaccgttca ggctggactg tcaggactat ctgtacacgc attttcagca ttctgagagg
     8521 ggccatcgcc tccggtcgtc catggtggta tcgccaccat acggaatcac cacgagactg
     8581 gactggcctc gattaaattc tggagtcttt atgtcaggta aaaaaactat cgttactctg
     8641 ctccgggtat cgctactggc atgcccgtta ctgttcacga caccatcgtt tgcaatgatc
     8701 gacacgccat ccgtgaaggt cggcttttct cctgaaggaa gcgcatccgc tctggtgctt
     8761 gacacaatca acagtgcgga aagctccatc aggatgatgg catattcctt caccgacccg
     8821 gatgtaatgc atgcgctggc aaaagcgaaa aaacgcggag tggacgtccg tattgttgtt
     8881 gatgacaagg ggaatacgaa ccgggcaagt caggaagcga tgaaatatat caacctgctg
     8941 gacattcctc tccggactgt agatgccttc cccatccatc acgacaaagt catcattgtt
     9001 gacggaaaca cggttgaaac tggctcctat aacttctctc gtgctgctgc acgcaagaat
     9061 tcagagaatg tcgttgtgct caagaatatg ccggatgtag ccgcacaata tcttgaacac
     9121 tggcaggatc gctggaataa gggtacagac tggaaaccct gacaaacatt tctcccccga
     9181 ttttctatcc ccccagccac ccccggcttc cggcggtggt ttttttccgt cttttttcag
     9241 gagaagcact catgccgtct gtatcccgct accgcacctg gcttgccgtg cctgcagatg
     9301 aaatagaaga tctcaaaaaa gcacatcccc cgatgaacgg gcacacgccc gtcatctggg
     9361 acaaggagca caaactctgg ttcgcccgcc cgggggcgga cctgtccagg ctggaccgct
     9421 ggctgccccg gccacaggac gtctccatga acggcagtga tccggtcacc gagtttgccc
     9481 aggttctgga gaatgccgga ctggttctga aagaactgcc ggtcatggac gggaaaatcc
     9541 accgtgtccc gaccgccgat gacaaaaaag gacaaaaaag tggcgcatat cgcggattcc
     9601 tggacggcag acctgccggc tggtacaggg attatcgcag cgcggatgat tcgccgatca
     9661 cctggacctt ctccggcgga gaacagacag atccccgcgc aagactccac ctgaaagcgc
     9721 attcaatgca acgcagggag gacgcggagc gggaactgaa agcacagtac aaccgacagg
     9781 ccgcgtatgc ccgccgctat gtcaacaaat ggccgcaggc cacagcacat gaatacctga
     9841 cccggaaagg cattcaggct gcaccggggg tgcgggtaaa caacaaaaat gaactggtta
     9901 ttcccttcag taaccgtaac ggcgcgatac gctcttacca gcgtattccg gtcaccgggg
     9961 ggaaggatgc ccgcatcctg aaagattcag agaaaaccgg taactggttt gcgctgggca
    10021 caccgcgcaa cggtcagccg gtactgtttg ccgagggcta tgccactgcg gcctccctgc
    10081 acgaagccac gggccttccg gtactgatga cggtggatgg aggcaacatg attgccgttg
    10141 ccgaaaatgc ccggcaaaag tggacgcaaa gcccctttat cttctgtgcc gacaacgatc
    10201 atgcaatccg ggttaacaaa ggtatcgtca gtgcaacaaa agccgcagaa ctgacaggag
    10261 gaacggtcat ctttcctgcg ttcaccgatg ccgaaaaagc gcaggggctg accgatttca
    10321 atgacctgga cgcaagccgg ggacgggccg cttttcagca tgtcatcaac gcccagttag
    10381 aacacatcgg ggtttccacc cccaacagta acaccccgga aatccgcgag gcactggtga
    10441 tcggcaacct cgtttttaca ccggtccaca ctgaggaaaa aaccatgact ccgacagaat
    10501 atcctgaaac atcacccgat acagggcaca gccatgacca gggaccttct ctgccgtccg
    10561 ccacgcagca ggaacaacca gctgcttcca gcagtaacat tgctgatgaa acacagtcat
    10621 ttaccagtca cgccactgaa aataacggca aagatgagcg gcacgctgat aacgttcagg
    10681 cgcaaacgga aagctctgta gttcaggact cccctgcaga atcaccggcg tccccggaaa
    10741 gtacaacggc atctgcccct gatgaacccg ctcatccggc ggaacctccg gaaaaagttg
    10801 tctcggtggc cacagataaa gactggcgtg aatttgaagc cgaactgtca caaccggaaa
    10861 aaaacgagtc acaacaggaa tccggcactt cacctgaaat accggcccct gctgccgccc
    10921 ccgcatcccc tgaagacagt ccctcatctg aaaccatgaa tgaatcagcc gagccatcac
    10981 cttcccctga tgcagtcaaa gaggaaatga ccgtgatgac ggagcctgat gacaaacaaa
    11041 gcaccggaat atcccagggt actccccggg aagaggcggt caaccatgta actgaagaca
    11101 ttccccttca gccagagccg ttccttcctg agtcagatgg ccccgaagaa tacgaaaact
    11161 acagcgcata tcaggagctg atgaacaatg aaaatcctga gcatctgcag gaaaacagtg
    11221 atatgccgtc gcaaccggac acagaccacg ttcaggcaga tgagtcagaa cttcaggcca
    11281 caacagatac tgcagaaccg gctgtccggg atgaacccac tccacagcaa cctgttcaga
    11341 gcacatcacc ttctgataat acttcatcgt ttctcgacaa agcgcgtggc ttttttacgc
    11401 gtaaaaaaac ggattcacag gcacatgaga acagtgatcc cacgccctca gcggaaacca
    11461 cgacgactcc accgatacct gacagtattg tctacgcccc ggaacgttct gatgcaccga
    11521 tatcgctcaa catggacgaa attcttaaag agctcgactg ggaaaaacga gcagacagaa
    11581 ccgtcctgta caaactggat ggcaaacctg ccttcattga ccgggtaaac cggctggaaa
    11641 tggtcaacgg tgccagcaat gacgatcgca gcgttctggc cgcacttgct gtggccacca
    11701 aattttatgg gggcgtcata gagctgacgg gcagtgatgc ctttaagcaa aaagcaatgc
    11761 agttgattat cgagcacaac atcgatgtcc ggatgaaatt gccagatcaa cgggctgaac
    11821 tagaaaaatt acgcaaggaa atggctgtca gtaaggatgc tgttgtcacc cataagccaa
    11881 caccggaact taaccggaat acccctgaac aaccagcagt cccagaccct gttcaggaaa
    11941 aagaagccac acaatccccg gcgtccccag tagcccctga agcatcaacc gtcagcaccg
    12001 tccctgcatc atcaccggct gaacctggca aaacggcaga tgcggcacaa ggtgaagaac
    12061 caccaggaaa acttcgcccg ggagagagcg tgactgccgt actgcacaat tttggtcgtg
    12121 ctgaatatgc acccggcaaa ggagagagtt tctttgttga gcttaaaaac cgcagtggca
    12181 gcaaactcta ctggggggag caactggaaa gtctggtcaa aaaccatcag aaaggtgacg
    12241 tggtgacact gacacttcag aaccgcgaac aattcatcct cccgggagag caaaaagcac
    12301 gtttccggaa taagtggtcc atggagtcag ttaccaacgg gatctcggta tcacatgata
    12361 atccggataa aggtcagcgt attcaggcga ttccggtcga aacctttatg aaagtggctg
    12421 cgcaaatcag ccaagggtgg cccgaagaga tgaaaacgtt acgtatgcca gaaaacgtcg
    12481 gctcacatct gtttatcggt gaagaccgac atccggtctc cgcgccacag aacgcaaatc
    12541 aggtaacaga aataaccagc gcagcccctg acaagctgac acctgttctg ggatcagtgg
    12601 ataaggatac gcgtgaactc aacctgctac ttgtccagag tgccgatgaa catctgcagg
    12661 gtgtggtccg gctgaacggc acattgtatc cggcactggc caccccatca gccgacaaca
    12721 gtcagctggt tatcaatgcg ctgaccgaca aaggtttacg ctttgcaggt tatggtgaag
    12781 cggtaaacca cgatgctgac agcacgaatc gccccgcacc ggagcttatg cagtttcatc
    12841 tgaaaacccg ggaagaaccg ctttttgctg ctgtctacac gccggaaaaa caacctgatg
    12901 cgttgtaccg gaacctgggc ttcgagcagt catggcagca gtggtccaac agccaaaagc
    12961 cggaggacag gcaggaaaaa actctgcatc aggatctctc ccacagcccc ggccgatgag
    13021 ccggtaagga agccatatga acatgcataa gtctctggtt gccgtgctcg gccttatcct
    13081 gatttcatcc acggcaagtg ctgcaacctg tcgtgcgggt agcgcggcac aggccggtag
    13141 taatacagga tatgagcgag cacgtgcagc tgcggatgca tggtcacaac gtgaaaacga
    13201 tgtttcctca tccctgcagt cctgtctgtc acgaatcaaa aaaatcagca taaacctccc
    13261 ccagttcccc agccttgacg acattctgtc gcagctggaa aaccaggtct gcgacgcggt
    13321 cgtggacaag gtgaatgaaa agctgccggg gaatattgat ccctggaagg actacaacct
    13381 atgaccgaaa ataccgagcc atcctcgcca tcacatccgg cacctgaccc ggaaatactg
    13441 aagagcacaa tcagggctat gaagcagtca gaacagcggg caaataatgt ccctgcgctg
    13501 ataaaagcgc ttttatgtac gggcacatgt cttcttatca gtattactgg taacgccatt
    13561 cagtactggc actcaacgaa tgtggagcgc gaatatttcg ctacagacaa tggtcgtctg
    13621 gtcaggctgg caccaacaaa tcagccagca tggagtcaga atgacgcaat ggcctttggc
    13681 agccaggcac tggcgaccgc attcaacctg gattttgtgc actatcgttc acagatttcc
    13741 tctctgtctc cgcgtttttc tgacgaaggt tttgccggat atgtgaatgc attacaggca
    13801 tctaacattc tggagacgat caagaaagag aagatgaatc tgaccgccac gacaggcgct
    13861 ggcgttctgg tacgacaggg acaaatgtcc gacggcgtat ggttctggac atttcagtat
    13921 cctgtccgta tgcgtctggt cggccagaca accagcaagc cagaacagtc ttttgttttc
    13981 gaaatcacca tccagcgtgt tgaccccaga ctgaaaccat ccggtatgga aatccggcag
    14041 atgatctccc gtaacgccgg accgaactcc tgagataagt aatatgcaat cacgttattt
    14101 actgagcaca ctgctcctgg tgtgcagcgc cgccacgtct gcagataacg caggatggca
    14161 gaacgcacgc acaccacaaa cgacaaattc tgccagcgat cacgttcaga acgcctccca
    14221 aaacaccgga ggtgttccgg caacactggt aaaaggtgag ctgcctgccc ccgggcaggc
    14281 cagcccgctg gtacaggatg cggcaagact tgattctgag ctctccgccg atgagattcg
    14341 ctcactacgc agtctcatgg cggataacga gcgggccatc aatgcgccca tcaccagtgt
    14401 tgtaccccgt atcagctctc tgacagttaa tctgtccccc ggtgccagcc ttcctcttgt
    14461 tcgcacagcc atgaacaacc tgagcgtagt gacatttacc gatatcaacg gttcaccatg
    14521 gccacaatcc gatcctcctt ataacgcggc accaaaactc tttgatgtgc agtacaacga
    14581 gaacatggtg acaatcacac cattgcggcc atgggcatcc ggaaacattt ctgtctacct
    14641 gaaaggtctg tcggtaccgg tcatcctgaa cgtaaccagt ggcgaaacag atacgccgtc
    14701 atccagtcag gaaatggaca gtcgtctgga tctgcgaata ccacggcagg gaccaaccag
    14761 cccggttgtc tccatcccga cggataaaat cgccctgcat gatgcaacat tacaggcatt
    14821 tctggatggt attcctcccc gggacccttc agttaagagg ctgaaattca caggtaatgt
    14881 cccggataca acgatctggc aacacggcga tgacctactg gttcgttccc gggcgatgtt
    14941 gcgcgatgaa tttgaacaga ccttgtcctc tgccgacgga actcatctct ggaaacttcc
    15001 cgtcacccca ctactgactt tctctgtgaa tggccagtcc gttcacgtga caccagaact
    15061 ggagtaatca tgagtgctga acaggatgcc ggtaaatccg gaaagaaact ggctgccctg
    15121 cttggactgg gcagcatcat cttatttggc gtcggctata tcgcgttttc caaactatca
    15181 gggagtaaca gcgatatgca gtcagccgtg aacattaaca gtgcagcaag cggcggaacg
    15241 cgcagcgtta ccgaaacgcc tcactatcgt gaactgttaa gagctgataa tgaacgaggg
    15301 gccgcagctg cagcccgcaa taatcagacg ttcattgcca gtcttccaca ggggcttgat
    15361 attccggaca ctccggaaaa gcaacaacaa cctgcagcaa aaccggaaaa ctatgctcat
    15421 cgccaagctt ccggcacacc acaggaggat cgagcagcca gtgaaaaacg tatggagcgg
    15481 ctgcaaaagc tgattgtaag aataaaagat cagcatcctg ccggtagtac accaaccata
    15541 gcaacggcta tgtggaataa atctccggca gaaacaacag ggcagaatgg tacgcagcaa
    15601 ttcgcattac agaatgccag cctgtccacg ccggtagcag aaaaagggat acaactgata
    15661 cctgctctga cccgtatacc agcatatatt gatacggcag tggattctga taacccgtca
    15721 tccaaggtta tcgcaaccat tcccgctggc ccctgggcag gggcaacgct gttcagtccg
    15781 ggagtcaaac tggtgggtaa cggcgtggag atccatttcg accggatgag ctggaacggc
    15841 atggacctta aggtcaatgc atatgctcaa cgggaagaca acctgatgtc atccgtggcc
    15901 agcaatgtga atacacgctg gtttaaacat atcattctgc cgtcagtgtt agggggtgtg
    15961 ggcagtattg gcacacttta taaagatgcc aacacgcagg ttattcaggg taactatggc
    16021 acggttaccg gacgcgtggg aatgcccagt ggtgaagctg ttgcgggcgt tattgccggt
    16081 ggtatggcgg aaagaggaag tcagatatta acccgccagg cagaaagtga gccctataaa
    16141 caggtagaag tgtatcagca tgaagtcgta tcaattctgt ttgttgaccc ggtaatgaca
    16201 aacgacgcca ggtcatcttc cctttccagt ggcatttccc cttcagttaa tcgtacatca
    16261 cagacggaac aacgctcgca ggcacgaatg cagacagcca tggagcaaag aaaagctgtg
    16321 atgcaacgtc ggtatgatga acaaccggag acaccataat gaaacctgaa acagaaatag
    16381 atgaaaccgg cagcttcggt gaacccgaag agaaacccgc ccctttctgg aaacgttccg
    16441 tctgggggat atctgtagca acatgggggc tatgtgcagt cgtattgctc gcagccatat
    16501 ggtatctgtt cctgagagca ccatcggaaa caggcatgcc tccgtttaat gatgcggatg
    16561 ctggcgtaca gacctggcaa accacacaag aatcatcacc ttccgttcag tccactgaga
    16621 ccatgactgt ccggacagga gaagatatga gccagcttgc ccgggatgta aaaacagagc
    16681 tggataaccg cgatgaaaaa attcaggcaa cactgaatat gcttcacgac agcattaaca
    16741 agctgggcga agctataaaa aaggatgaag aatacgcaca ggaaacgcgc cgtcagctgg
    16801 atgatatccg gagccacctg aacggaatga tgacacagaa aagcgtcacc gaatcctctt
    16861 ccacaccgca tccagcaaag aaaaaaacgt cgtctgtact taacggaatg aaaatcatgt
    16921 cgatggaaac tggcatggcc tggattcgct ggcagggtag tacctgggct gtcagagaag
    16981 gtcagactct gggcaatgtg gtgatccagc ggattgatcc gacgacccgg accatcatca
    17041 ccagtgcagg gacgttacgc tgatgaacat ggatgccctg accgccattg agaactttgc
    17101 cagcagtatt ttcagtgccg gaatggattt cctgtttacc tggggagaat tcatcggggt
    17161 gatcagcatg attaccctgt ttgcccgcgc ccgttcagcc ggtccggtaa aaatgtctcc
    17221 cggaaaattt attgcgggga tgttaaccag ctgcatgctc gtatcactcc cggcaatgat
    17281 taacgccgga ggcgtacaaa tgggattcag ggctgactca tttggcccga ttgcatatgt
    17341 acaacctcag acgtttggcg ctgctgctgg cgcagcaaat gcagttctgt cgctggccaa
    17401 gctggccggt gttggatttg taatgaacgg gatatccatc tggcgcaagg ccggactcga
    17461 tggacatacc gcactgagtg ccagtgaatc cgtcagtaag ggaaacgtca aatttattgc
    17521 cggcgtcctg ctggtcttta tcgatcgtgt cctcaatgcc ctgctggcaa gcattggaat
    17581 cgtcttctga tttttttcac cttcatataa accactgata tatccattta aggagaaact
    17641 atgtcacgta ttcgtgtcgt agcggcccgt ctgtacgccg catttatcca cgcacaactg
    17701 tttgtaatgt catgccggag caaactgact aagttcctgc tgctcattcc catgatgttg
    17761 ctgcccaaag ccgttcttgc cgatggagat cttgccgata tggttcgcaa tgtcgaacaa
    17821 ggtgccaaaa cagcacagtc atcatcactg actattgcgc agttcatcgg cgtaattctg
    17881 tttctcgggg gcctgattgg cctgaaaaaa gttgggaaac aaggggggat gggactggct
    17941 ggttgcattg tatctatcgt cattggggcg ctacttgtcg ccggacctga aatgatgagt
    18001 cgcagccaga aacagctggg gatttccaca atttccattg gctaatcttg tccagggcac
    18061 tggcatttat ccggtgccct tcctgttcgg caatacaagg aggcacatat gtctgcctgg
    18121 agcccggagc acataacgtt acgacgaaca gaaaacggca atgtgattgc ccacgacagt
    18181 cgggacgatc aggaattcat ttttgcagaa tgctgttccg atgaaatcgc aaacatcatt
    18241 ctggaagccg tgaaacgtta caccagtacg gagcctgctc atgcaacaca gcattaaaga
    18301 cttgtggttg tatccctttc cagaaattga tgtggttcat acacaggagc cactgttacc
    18361 ggaaccggaa ttaacaacgc ccggtcgatg tatctgctgc cggcaaaacg tcaggcaccg
    18421 tttcagactg gatgactcat ggccactcag acagcttacc gacacaatca gtgacacacg
    18481 agttcgtctt aataaggcca cagaacatct ggataagtta aaaaagcgtg gtgaaccagt
    18541 cgcaaccggc gaaaaggaaa agtacaacac ggcagtaaaa gccgctgaac gggcgcttga
    18601 gcaggcccgt ctgtctgcca gacgtctttc actccgccat gtacagaaag ctgaaatcac
    18661 cagtacagag tcgttgtcag agaaagagca ggaactgttt catgaagacg gcccgcctta
    18721 cagcttatgt gctttctgcc acgcctggca ttcactgaat ggttataccg cagcacaggg
    18781 cgttatggtg tggctacccg accttcatcc atccacggtc gtggccctta accgtcgttc
    18841 attacaggaa gttttcagta atgataagtt ccgggtccgg cggggacggg aagcgctctc
    18901 tgcactgatg caaaacagac tggcggttga ggataaattc cgcagtttcc gcccggccga
    18961 tttcgctgac gtttttcgtc gctatccacc ttccggtcgt tcgcccctgc gtgaaaaaat
    19021 gaacgggatt gccctgattc tgacaccaga ttcattcatc aaaaaagaat acgttgattg
    19081 atttttcttc atgccactcc agcgcggggt ggcattaaaa aaatcaaact gcacaaacct
    19141 ccctcctgta agtccggagt tccctcacct atgaacataa accgtttaat cttcactatc
    19201 gaagactgtc tgaacacgct atcccgtttc tctgtcgcaa gcagttttgt tgagtattgc
    19261 gatttacgta ctgtaatagg tctggacaga caggatcgtg aacgtcgccc atggctgaat
    19321 tcgccctata ttgcagccac aaagcgtgga gaatatctct ctgtctttga agtcagcggt
    19381 gctttcaggg aaatggatga agcatccgac cagactggcc caggttcact ggaatcgctg
    19441 atcaccagca tgagtgacag cctgaatacg gcatataaaa acagcggtca taaaatcagc
    19501 ttcgtttttg agcgtgatcc tgacatgggg aaagaagaaa tcgaagacat ggtcgcccca
    19561 caaaagcgct cactggctaa tacaggtatc cagttacagg atgtggtcga tgagaaagtc
    19621 acaacactgt ccccatggct ggtccgggag cgttgctggc tggcgatctg gagtggaccc
    19681 gaccttatca gcaacagcga tcgcaccgcc catgatgagc ttgtcagaag actggctgaa
    19741 cgggtaccga aagcacgctt tgcccagtcc ccctggcagt ggaccttatc tgcactgaaa
    19801 atccgccacg aagcttttct tgataatgtg gaacaggcac tgagacattc atctgatggc
    19861 ctgattctcc ggctactgga tatacacgag gtcgggcgtg aaatacgtcg tcagacagaa
    19921 cggcacagca caccgcgtaa ctggcaaccc cacctgcctg aagatgccca gccagcggga
    19981 tatcgctgga ctgacgatga atccgtacta catgctcctt cattgcattt gcagttattc
    20041 aacactcagg tgaccacaca gggcaacctg gttcaggcgg gtggactctg gcacggaatg
    20101 gtatcgatta ctttacctcc gcaaaatctg cagacgttta acgaactggt ccgtgccgtc
    20161 ccccgcgcag tcccctggcg catcagaatg gatctgatgc ccggcgggat gaaggctctt
    20221 aatctgaaga aaacgcttct gacttacagc agcttcattt ctgccgttcg tccaatgtat
    20281 gaatccgtta tgaccctggc ggcaacggat gaaaaagaac ctgtctgcat aatgaccatc
    20341 atggcctcca cgtggggaaa aacacgtgaa atatgcacac gaaaccaggc cattctgaaa
    20401 tcggccattg aaggctgggg cgtatgtggc accacgacaa catttggcga tccccgacgc
    20461 gcctgggtga ataccattct cgcggcatcc ggaggctccg gcccggttcc cctgtatccc
    20521 ccgttgtcac atgccatctc gcttttcccg cttaacaggg caggctctgt ctggcgaggc
    20581 aaagggaatc tgatgttaca tacagaagac ggttccgcat ttgaggttgg tctggcgtca
    20641 tcccaacaaa ataaacatac agaactggca ccaggcgatc cgggactggg taaatcagtt
    20701 ctgattaata cgttgtcgga aatacagata tcatctgctc agaaaaacct gcccttcatt
    20761 gcctatatcg ataagggata cagtgctcag ggactggttc agcttattcg cgacagtctt
    20821 cctcaggaaa gaaaagacga agctgtcggt attattctga gcaatgatcc ggactacacc
    20881 cgtaaccttt ttgacgtgat gtacggcgct aaaaagccta ttacgccgga aaagaatttc
    20941 atgtcatcgg tgctgtgtgc tctctgtgtc gataccggta ccggtcaacc ctgtaacccc
    21001 ggagacaccc gacaaattat caaccagctt atcgagctgg cttttaaaga gtacggtgaa
    21061 aataatccgc gactttatcg cgcctcaacg gaagaactgg tggattcagc actgcaggac
    21121 tcgggactgt atgaaaaaca tgatgcagcc tggtgggcca gatcaacctg gtttgaagtc
    21181 cgggatatgc tccacaatgc cgggtatatc atggcggctc agcgggcaca ttatcaggca
    21241 atgccccagc ttcccgaagt gtcctcgatg ctgggccata ccagtctcag ggatgttttc
    21301 ggtactgttc agcgtgatgg ttcaaacgag cttctgctgg actacatccg ccgcgccctg
    21361 gagcagggac ataacgacta ccccatgata tccgggtata cccggtttat gatcaacccg
    21421 gaaacccggg tcatcgctgt cgatctgaat aatgttgccg gtgacaaaac gcctgcaggc
    21481 aggcttaaaa ccggcatcat gtacctgctt gcaggtcaga tcgccggtgg tgacttcacc
    21541 ctgccacaat acagggatga agtgttaaag cagcttccac gggaatacca tgaaatcgct
    21601 cttaaacgta ttaaccaact tgatcaagag gttaagacaa aagtttatga tgaactgcat
    21661 aacgcccggg gcattgactt catctgggaa aatctggata cccaggagcg tgagcaacgc
    21721 aagtttgcta tccgcacggt tctgagtacg cagtatctga gggactaccc ggaaagcgtt
    21781 cttaagtcag caaatactct ctggttactg cgctacaaac ccgaagatat ccccgtactg
    21841 agggataact tcaacgttcc tgaatttatg ctgaaacgct tcctgaaaat gccggaaggc
    21901 ccggcaccag atggtagtgg tgttcctgtt ctgggagtat tcagggtaaa aagtggcacg
    21961 cttgcgagaa tactcaaatt taccgtcggt ccactggagc tatgggcgct gaactcatcg
    22021 ccaaaagaca gtgccctgcg taaaacactg acaaataagt taggttcagt tcgggcacgt
    22081 aagattctgg ccgaaaactt ccccagaggt tctgcaacgt cattaattga gcaccgggcg
    22141 ggccaacata acagtgacaa tgttattgaa gaactggcct cggagctcat ccgaaaacag
    22201 ggatacaatc tctgatgtgt acaattcata tcaccccacg ctgccggaga cctgccagag
    22261 ggcaggtatt tcttaaaaaa cttgaggtaa aaggtgacag tttctcattc actctgccgt
    22321 cacgtgtctt caggttacat ccggccccca atcccagagt gatatacgcc tcggatgcca
    22381 gaacacaggg aaaggttaca ggcatgcggt tgttgttgcc gtccagcaca gatgagctga
    22441 taacaacggc aatatggcag gtttctgtcc cggatgaagc accattcgac gttcagcatg
    22501 tcatccagtg gatactccct tcaccgctgg cttcgctgat gtccgatgat acctggttat
    22561 ggccatccgt tcccggaacg ccggcactga gtgccggtga ctggcctgtt atggatactg
    22621 acccttccac cttacggcgc gtcagacgtc gttctttcag catcatcgat aaccggaaca
    22681 ggaaaggtta tatcaccagg cgatttcagt gtttcccact ggccccacta cctgaaaaca
    22741 gaaaatacga cagccttcct gaccttgtgc tccatcccga gcaggagaca ataaaatgca
    22801 aagaaaaaca ttactcgcag cccttatagc caccctgagt ggaacggcat gccaggctca
    22861 tgcttattca gtcaccgtcg tcgccagccg gcctgttgaa gagcaggtca tccccagaat
    22921 ggaagccatc aaagatgtcc ttggcaacat actgagtacc cagacagcca cgggtaccgc
    22981 tatcaatcag aactctgaaa agctggcaag tgtcattgcc cagaacggac aggcgacacg
    23041 tcagcagatg attttcagta atgaaactca gcgcctcgag gaggcacgga aaagcttcac
    23101 cgtgccagac tccatctgca gtgaatccgc atcagggatt gcaacagaaa gcaaaagtgc
    23161 ctcagcatcc gcagcctcaa aactcagtaa aggtggcggt gtgagcaacc gtagcatccg
    23221 cgaccgtctg gccagcgcag caaactctcc cgtgcgggaa gcttatgatg gcgcggccat
    23281 acacgccagt tactgtacag aagcagaata tgcacgtttt ggaggaaccg ccgtttgtcc
    23341 ttctgtgggc gaaataccag gtggagacag tcaggtacgc tcgatttacc acggcgcagg
    23401 tacagcggac acacctgcag cactgacatg ggatcagaag caaatagatg cagcgacggc
    23461 ttacatgaaa aacacttccc gcccctctgc cggtcgtgct ctgggtaaag gagaagtgaa
    23521 tacccagagt gggcgaacct acgtcggact ccagaacgaa tacaacggca tcattgattc
    23581 tgcgtcgaat ccccaactga cgctgattgc tgacagcacg cctaatgaaa gcacacgtaa
    23641 agcactggct gaaacactgc agtcagactc agcggccgct tacttcgacc aggtggcttc
    23701 accagaagca aaagcacggg gatatatgag tacccgtgag tttgaagctt ttgaggctgg
    23761 ccggcgctac gccaacacag catacctggt ggatttacag gaaatgcagg gagataacct
    23821 gctccgtgaa ctggtaagaa tcactgcgca aatgaactgg caactgaacg acctgaaaga
    23881 acagatccgc cagggtaatg tcatctctgg acagcaactg gctctgaccg cccgccagta
    23941 ttatgaaaaa caactcggta gccttgagaa aacaatcaat caggcaaacg cacgttgaaa
    24001 gctgacgtca gaacaggact gaaaaaatga ataagttacc ggaaaattca gaccagacga
    24061 aaaacgtcaa aaaatcacca tccataaccc gcatagccac ccgggggatt attaacattt
    24121 ttgttcctct gatggagact cgcctgataa cgaattctgt acagcatatg ctggaacaac
    24181 aaaaacgcag gctggacaga tttcgtcagg taaataataa aaaggcgcaa ctccacctca
    24241 cgtgggaaga agctctgcag gccagccata tgtcagtaga cgatctggat cgtcgtttcc
    24301 gccggcgcag aacagtatgg cgattctgtt gctggagtct gctggccatc gctttgtttc
    24361 tcagtggtat gctctttgcc gcctcttcat tacctcttac gactctggta cgcgccatca
    24421 gcactcttgt gttgatactg tcaggcgtcg ctctttgtgc ttccagagca ctgattgtta
    24481 cataccgcct ctggcagttg catgaacgaa aagtcagcga acccgagcag gggactttcc
    24541 gtaattttct gaacgaccgc aatggctggc gcaatgccac attaattgcc gtaaccagta
    24601 aacaatactg atcacgtcca gatgccagta ccactggcat gtcgccatcc cccttaatta
    24661 aatcctgtgt ggcctttgcc catacgcaat tctattccgg agttactgtg aaaattttac
    24721 ttcgcgcact ctgtgcaggc cttgctatat cctcactgcc ggcaatggct tcagtgacct
    24781 atcaggatat tgtgtctgcc gcaaccaatc ctgacgatct gtcccgtcag gcgctggtca
    24841 cgatattcgg tgatgtagtc accaatccac tgtcaaccag cgcccccact ctgatcggaa
    24901 gcatgttcgg ggcgttcaac agtatcatcg ccgtacttgc cgttgtctgg tttatgttca
    24961 ttggtatccg tcatgttgtg cgttcggggc atcagggaca ggtattcagc accggacgcg
    25021 atatcgtcgg cacactgagt gtcgttgcag gttttctgat gatcgttcca accggaaatg
    25081 gctggagtat cgctcagctc attatgttat ggggcgcatc gataatgggg gttgggtccg
    25141 ccaatgtgat ggtgcaactt gccgctgata atatcgcaaa tggttattca atgactgtac
    25201 agccagttca ggcatccaca cgaacagcag cgcgtggcat ttttgaaatg gaactgtgta
    25261 agtatgcggt taacgcagga ctaaatgatt ttaatcagac ggtcaaatcc tcgacttcac
    25321 tgatgacaga atcagctaaa accgccagtg gtaattacac tgtcacggta tccaatggca
    25381 gcggtatctg tggttcagcc agcctgtcag tagaaggaaa cggaacaaca gatcaatcga
    25441 cgattggaaa attctttaat cccttcagta aaaacgaata cagcggtgtt atctcagcac
    25501 aacgtgcagc catggataat atgattagcg acatggacaa cgctgccagt gagtttgtga
    25561 caacatttct ggaaaaaaga aattctggca acggaacatt gccggatatc gagacacgga
    25621 ttcagagagc agccgacgag tatgaacgtg ctgtacaaaa atcattacct attgataacg
    25681 gagaacaatc caggaaagaa gctctcaagt cttatctcac aacctatgga tgggtgacgc
    25741 ttggcgcgtg gtatcagacc tttgccacag ctaaccagcg tctggcagag ctggctgaca
    25801 gagctccagc tgtaacttca atgtcatcac tgggagaagt tggtgatact gacttattca
    25861 gtgcggtcat gagtgcctat aggacgcaac tgcagaattc ctcctatacg ccaccgctgg
    25921 gcacagtagt atcatccagt gaacaacgca tggcaaatgc acaagatccg aaatctgcct
    25981 tgtctgaaat tatgggcccc cttgttagtc tcactaacag gattgcaacg gaaacaagtg
    26041 gcacaggcac aaccagtgct caaattaatc ctctgataaa aatgaaaaat atcggagata
    26101 taacaatggt aagtgcagaa ggaatctgga ctatatatac aacggccaga gttgtcgttg
    26161 ccggtggcaa agattcaatt cttgtaaaat tctttaactc actaacaggt gccgcttcga
    26221 tgcttaccgc gctgtttgaa gcacttgcac cacctgttta ttttcttttg ttcctgatgt
    26281 tctgtgctgg attttcctta tcaatttacc tgccattcat tccgtttatt ttctggatga
    26341 caggcatcgg gaactggata gtcagcgtat taataggatg taccgccggg ccgctgtggg
    26401 ctgcaacaca tctcgggaca tcagaagaca gaggaagtcg ggcagcctac ggttatatat
    26461 acttaattga tagtatgata aggccgccca tcatggtctt tggttttttc tttgcttctg
    26521 tagccatcat cgccgtaggc acaatactga atgcattatt tggcgctgcc ctggttaacg
    26581 tacagttcaa ttcgctgacc ggtattttca gtcttgctgg tttcttgctg atatacgcca
    26641 gaatttgtac aaccactgta gctgccatat ttgcccttca ggcctaccta cctgatcatg
    26701 ttattaactt cctaggggga cgtgatggag ccaacacgct tggcagcatg gcaaattctg
    26761 ttaaagaaat atttattgga ggaagtcgca atatccgaca tactcctgga atgaaaacag
    26821 atcgtcttaa agataataca aaaggaagtg acgacaagga cggaataaaa ggataatatc
    26881 aatataaata ttgcacaacc tccccatcgg cattaaaata atagatagta cgaggggagg
    26941 cattatatga aagctaaaag agaaacaaca gacagatttc caacctggtg gctattttat
    27001 tatgtattaa gaaaagctta tttctttctt ggaatccctt tttttctgtt ctgcgcatta
    27061 ggctttaccg aaatgctatg ttctgatcgt tatttcggaa ataaggtcga agattatgtt
    27121 gtcacttttg gtagttggtt cttattactt gcgccaggca tatggatgta ctcacgtgca
    27181 aaaacaagga gagaaaaaat aagaaaagtt gtacaaacaa taaaagagtc aggcttctac
    27241 tctccagaga aggggtatga aggattatct ttaactcagg gagcttattt cggtattgac
    27301 cttaaaaatg gcacaatgtt gtatgtccgt atttatccag gcaacattat ggatgttatc
    27361 ggttttgaca ttcataattt cactcgtact gtaacagacg acaaaacact ggaaattcat
    27421 acgaaataca tcaatctgcc aatggttcct attccatcat ggtgtactca tccagaaacc
    27481 gcatcaaaca ccatgcacgc tatggcaagt cgaggatatg attaccctgt cgactttcct
    27541 cgtctgattc aggaaaagcg taaggagtgg gagcagattg caggcgtgcc tgtagccgaa
    27601 gttttttaag ataagctccc cccataacag ccggttcaaa tgagccggct tttgttcatt
    27661 acaacagata acttatgccg ttaccgataa attcgaccaa gtgctttaat cgcagcaatt
    27721 tttatttcca tatcatcact ttgactcaat ctctctaatt cactaatgat attatccgcc
    27781 tggcatcgac cttcacctaa tgcatagatc gcagcaagcg taatgtcttt tgatttactc
    27841 ttcatcattt caaacaagaa agtattagct atcgcaggag gcatgctcat aatcaatcct
    27901 cttcatgaaa taaacaatgg cactcactta caaacaggag tccactatcg caagcgactc
    27961 aacttagtaa tatctctcaa tttgaatgaa aaaaccagat ggcatgtgac agggaaccgt
    28021 ccgcatgatg tccgtgacca tttaacccca taaactgata aaaaatgaat accggtatct
    28081 caatgcgaag aaataatctc cgtagaatcc cccatgagaa aaatatcata tgggagaaaa
    28141 tcgcaattat cacagccagc gcattcatta ttttcgctgt gttctggcat taattatttt
    28201 caacacaaaa aggaaaaaac aatgaatctg gtagacgcct ttgtcaaaaa agtaatatcc
    28261 ggtccatatg aagaatatgg aaaatggtgg atagatgttg aatacatttc atggggtgtt
    28321 tccgggaaaa ctagactgat gtttgactca aaggagcagg cactggaagt taaggaaggg
    28381 tacaaatttt taacgtgaga tctctttttg ttaaagggca ggaagcggta actccctgcc
    28441 cttagttacg tttccaggct tatcagtcga caagttcccg gatcagttcc agaacagcaa
    28501 tcagcgcgtt tgcaacggca actactgccc tgagaacgat gattaacaga ttaatcatcc
    28561 gcttctccat cactggagga aatcaccgtt gcttactctt acattgcctg tcacaacggc
    28621 actttcagtg ttagttgaaa agagacttcc tcctgccaga gcactgaaca cagcaccacc
    28681 tgtttgttca gccaggactt aaaatcttgc atccatagtc ccgatggata tcacggcaga
    28741 agtgtcgcca gacagagcta acctgcggat tataagggtg gttgctatca aatgaacagc
    28801 tcgctattat aagaacgtta gttgaaaaga gatttcctcc ggtcagggcc acccactctg
    28861 taacggaaca gtaaaaaaac cgacctcacc agtcggtttt tttacatctg ttgtttctca
    28921 aaatccgccc aacatgcccg tcacaccggg gaaactcatg ctctgaatca gaaccaacca
    28981 tctcaacttt gccgtctcct gaaaaaactg gcacattcag ttcgataact acccataaat
    29041 acagagagaa aatccgatgt cagaaaagtg gagtaaagaa ttcgactctt gctttcaggt
    29101 ggggagcata gaagaaattg cagatgctct cgagaaacgc agcggaagcg aggaaaatgc
    29161 gttagccatg ctggcaatga cagccttcac actcatgacc cgacgtggta tctgcgaaat
    29221 gcagaacgtg gcccccgatg gcaaaggcat tcggctggag ctaatcggct tcagggaaaa
    29281 tgaaacaata ccggatacgc ttcattgaag aagaaagggc gcaatgaggt cagccttcgc
    29341 aacaaagcgg cggcagaaaa cctgccgccg tgttgcccgt taacgtttaa cttcgtaggc
    29401 taacgttgcc acaagcactg tgtttccctg ctggagccgg agtccgcaaa gcgaatccct
    29461 caccatccaa acaaaacaca gaatcgtcac acagatgacg attaacatca ttaaaaacgt
    29521 tcgctgtggc atcatagccc ctccattgcc ttacggcggt tatcgggcta ctataatgtt
    29581 gtctaggcat tacagtagag cccttggttg attgataaaa aaatctccag gggctttctg
    29641 ctgtataccg tccacaaaac gcttttggca tacagcctca agcgcccgca gctattctaa
    29701 cgactttcgt atctgcaaca atccttttat cgcacggcta attcctctgg ttagctctta
    29761 tcgcctgcaa ataaattttt aacctttccg tttgatttaa attccagaaa tggagacttt
    29821 gtcatgtctt acaatcgaca gcctgtcgca gaagatccta tgcagatatg gggcgctgtt
    29881 ggtgtccttc ttatcctgct gctattcgtc atatggttat tcttgccgga agtggtttac
    29941 gccagctgcc tgattcttca taccttatgg ggactggtcg actgggggcc atttcataac
    30001 tatgcagcac cacgatacaa cctgcttgcc atgaccggta acaacgccgc caacataagc
    30061 tatagccagt gggttaacgt catggagcag accataggta ttctgtggat gtacctctta
    30121 cctgtgacac tctggtgttt gtgggaatgg tatcaacatc cgggacaaag ccgtttcacc
    30181 cgcagaccag tagatattac ccgactgcca cacatctttg cttcgttgtc ccctgctatc
    30241 gcccctgtac tggcagatgg tgatccggaa aaactctttc atggtggcaa acgacctgaa
    30301 cggcgggttg ctctcacccc ggaagcattt gtcgagcagc atactctcat caccaatatg
    30361 cagctcgatg ttgcagctgc aagacgatgt ttcatggccc agttgggtaa acctttaacg
    30421 tcctggaaag acatggcacc acatgaaaaa gcgctctttg ccatatttgg tcttcagtat
    30481 ttcctggatg atcgtaaggc agcattaaag ctcatggata cactgaacct ctcatgcaga
    30541 attaaaagca aacgggacag tgggaaattc tgtacccctg tttattcact ggccaaatca
    30601 gctttccaga gagtgattaa aagcaacgaa gcacagcaat ggctgaaaca acatcgctac
    30661 gtccgcagtg ggctggtctg gctgtatgca catgacctgc ggcttacgcc acccaactgg
    30721 atttggctga aaggcgtgga caggacgctg ttttatgcat tacaccgcgc caataccacg
    30781 aaaggattta ttgaaggcgc tggcgtggtg gccgttgcac gtgcagaggc cgaagccatg
    30841 cgttttggcc ttccctgccc tgaaccctgt gttgatgaag cggtggaagg cttacggcgg
    30901 gatatgctca gtctcggtct tatctgggat gaaccacaac cagatcgcga ccggaaacgc
    30961 cgcatcctga ccaactggtc cctcaccgac gacatcctgc cccgtacacc tgctacagac
    31021 aacgaattct gattatccgg agaactgaaa atgacaatcg agtactttgc aacacgcatt
    31081 gcaaaacata tttccgtgca ggcaatctgg ccgaatggca ggacagaaat tattgccgtc
    31141 cttccacggg atgccctgtc agggctggta acacgaaata atcaactgat tcatatagcc
    31201 tttgacgcca tgggcacccg taacagagaa accgtcctga ttgataaaca ggagcataac
    31261 gctgaccaga tacttacagc tattgattcc tgcctgcgtc ttgaatacct gatggaacgc
    31321 cgcttcagca gaacaccttt gttccgtggc ataatcgcca gcgttgtgct gttcgtaatg
    31381 gcaacgattg gcttctctct gttccgctat gttgatcgtg tattctggga tgatacaacg
    31441 cctgaagcag tacagacagc aggtgagcca cgactgcttc cccctcacct gaatcacact
    31501 gtgccattaa atgaaggcat tcaactcccg gttcccccga aaaaagacgt ccaggtaccg
    31561 gagaaaacaa tcacaagcaa aaatccggaa gccgcagctg cgcgacataa tctggctgct
    31621 gttctgaagc ggaacgccga tcggggcatg tttaccatca acctgtcatc cggtcatgaa
    31681 cgaaccctgt acgcatttct cgatcctgcc tgtcctaact gcagacttct ggagccggcc
    31741 ttaaaacgcc tggccagcga cttcaatgtc gttatctacc cggtttcagt tattggtgga
    31801 gaggaatcca ccgaccgtgt ggcaccactg ctgtgtgaaa aagatgctca gaaacgtgct
    31861 gccggctggc accgactgta ttccgcagat aacggaatga tgacgccatc agaagaaaca
    31921 acgcctgcag atgaaacatg cctgaaagcg gccagagctg caattgatgt aaacaatgtc
    31981 gccttccgta agttcggatt tgccggtacc ccatgggtgc tgtccgacac cggctggcat
    32041 ctgcctacag gaattctgca ggaaactggc acgcttaatt tattcctgaa aaccaccgac
    32101 agcgagtctg gccatgagtg aacatcgcgt taaccccgag cttttacacc gtacagcctg
    32161 gggaaatcct gtctggaatg cactccagag tctcaatatc tatggctttt gcctggtggc
    32221 aagtcttgtg gccagtttta tctggccact tgcattaccg gcatgtctgt tgttcacact
    32281 gataacaatg ctggtattta gtctgcagcg atggcgctgc cctcttcgta tgcccatgac
    32341 gctggaatgt gctgacccgt cacaggacag aatgattaaa agaagtcttt tcagcttctg
    32401 gccaaccctg ttccagtatg aggtcattct ggagtcaccg gccagcggca tattctatgt
    32461 tggttatcaa cgcgtcaggg atataggtcg ggagttgtgg ctgagtatgg acgatctgac
    32521 ccgccatata atgtttttcg ccaccaccgg tggtggtaaa acggaaacca tctttgcctg
    32581 ggcgattaac ccgctttgct gggcaagagg attcaccctt gttgatggca aggcacagaa
    32641 tgatacggca agaacaatct ggtatctggc cagacgcttt ggccgtgaag acgatgtgga
    32701 agtcatcaac ttcatgaacg gaggaaaatc ccgcagtgaa atcattctga gtggtgaaaa
    32761 aacgcgtcct cagtcaaata cctggaatcc cttctgttac agcactgagg cattcacagc
    32821 ggagacaatg cagtcaatgc tgccccagaa cgtccagggg ggcgagtggc agtcccgggc
    32881 catagccatg aacaaggcac tggtatttgg tactaaattc tggtgtgtgc gcgaaggcaa
    32941 aacaatgtca ctgcagatgc tccgagaaca tatgactctc gaagggatgg ccaaacttta
    33001 ctgtcgtggc ctcgatgatc agtggccgga agaagctatc gcaccattac gtaactacct
    33061 tcaggacgtt cccggtttcg acctgtcgct ggtcaggaca ccatcagcct ggacggaaga
    33121 acccagaaaa caacatgctt atctctccgg gcagttttct gaaactttca gcacattcac
    33181 ggaagcattc ggggatattt ttgctgaaga ttccggtgat atcgatatcc gtgacagcat
    33241 tcacagcgac aggattctga tggtaatgat ccccgctctg gatacatcag cgcatacaac
    33301 gtctgctctg ggacgaatgt ttatcaccca gaaaagtatg attctcgccc gtgatttggg
    33361 ctaccgcctc gaaggaactg attccgatgc actggaagtc aaaaaatata aggggcgttt
    33421 cccttatctg tgtttccttg atgaagtggg ggcatactat acggaccgta ttgctgtgga
    33481 agccactcag gtgcgttcac tggattttgc tctgattttg atggcacagg accaggaacg
    33541 tatagaagga cagacaacag ccacgaatac tgcaacactg atgcagaaca cggggacaaa
    33601 atttgccggc aggattgtca gcgagggtag tacggccaga acgctgaaaa gcgccgccgg
    33661 agaagaagcc agagcgagaa tgaacaacct gcagcgtcag gacggcatat ttggtgaatc
    33721 atggattgac agcccacaaa tcagcatcct gatggaaagc aagataaatg ttcaggagtt
    33781 gattgaactt catcctggcg agttcttttc tattttccgg ggagaaaccg taccatcagc
    33841 atcattcttc atccctgacg atgagaaaag ctgcagcagc gatcccattg ttataaacag
    33901 gtatatcagc gtggatgcgc cacgcctgga ccgtctccgc cgtctggttc cccgtactgc
    33961 tcaacgtcga atcccgtcac cggaaaatgt cagtgccatc attggcgtcc tgactgcaaa
    34021 accctccaga aaaagacgaa aaattcgcac agagccacac acgatcgtgg atacattcca
    34081 gcaacgcatt gcaggacgtc aggcggctat ggcaatgctg gaagagtacg acacggatat
    34141 caacgcacgt gaaagtgcct tatgggaaac agcggtgaat acgcttaaaa cgaccacccg
    34201 ggaagagcga cgaattcgct atatcacact gaacagacca gaactacctg aaacaaatga
    34261 ggaaaatcag atatcagtga gagcagaaag agccggtata aacctgttaa cgcttccaca
    34321 ggacaataac cacccgacag ggcgtcctgt aaacgggttt catcataaaa aaaacaaccg
    34381 tccggactgg gacggaatgt actgaactgt gaccatactt tggtcttccc gcaaaaaacc
    34441 tatttaaaaa tgctggtact agataccagc attcagatta cagattaacc acatcattgt
    34501 aggtacatat ggaacgtcta atttttatat tctttaaccc tagatcatct aattttgacc
    34561 gaagctgttc atcagattgt tcaaataaag gccccaacac aatctctcta attgccgtaa
    34621 tattgttaca ggtaaatttc accttagtat aatacgcaat tccgtaatca gtactacgtt
    34681 tttcaagttt gtatggtggg aatgtacgtt catctacttc aataagtcct cggacctcat
    34741 tttcacattc aaaactctct ctcttatata atgaacatag tctgataaga tgtgcatgaa
    34801 gtatgccacg tgcatattta atatttaaac ttgagcgatt aatataatca tgcaatgcgt
    34861 ttcttacata ttctcgaaac agttcttctg aatagataac aggatgaaag acaactctac
    34921 cactaatagg
//
LOCUS       DACBVI010000037        34076 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.037, whole genome shotgun sequence.
ACCESSION   DACBVI010000037 DACBVI010000000
VERSION     DACBVI010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.037"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            467..2485
                     /locus_tag="IU325_08560"
     CDS             467..2485
                     /locus_tag="IU325_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP5809084.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELSHEEILQLIDNFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYPNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVGLAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQSFDETILASGIVPRIPPIDGIDHPKVLSYLDVLRDKAPVGKKVAIIGCGGIGF
                     DTAMFLSQPGESTSKNIAEFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(2603..3739)
                     /gene="rlmG"
                     /locus_tag="IU325_08565"
     CDS             complement(2603..3739)
                     /gene="rlmG"
                     /locus_tag="IU325_08565"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HAP5809085.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            3824..4327
                     /locus_tag="IU325_08570"
     CDS             3824..4327
                     /locus_tag="IU325_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HAP5809086.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEQQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            4404..5096
                     /locus_tag="IU325_08575"
     CDS             4404..5096
                     /locus_tag="IU325_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HAP5809087.1"
                     /translation="MLRAFARLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNTS
                     KQLTWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            5175..6161
                     /locus_tag="IU325_08580"
     CDS             5175..6161
                     /locus_tag="IU325_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP5809088.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFAELVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            6445..7410
                     /gene="alx"
                     /locus_tag="IU325_08585"
     CDS             6445..7410
                     /gene="alx"
                     /locus_tag="IU325_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HAP5809089.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            8026..9270
                     /gene="sstT"
                     /locus_tag="IU325_08590"
     CDS             8026..9270
                     /gene="sstT"
                     /locus_tag="IU325_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP5809090.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVIF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(9274..9825)
                     /locus_tag="IU325_08595"
     CDS             complement(9274..9825)
                     /locus_tag="IU325_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HAP5809091.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPSAVDERG"
     gene            complement(9908..11395)
                     /locus_tag="IU325_08600"
     CDS             complement(9908..11395)
                     /locus_tag="IU325_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP5809092.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            complement(11410..12822)
                     /gene="uxaC"
                     /locus_tag="IU325_08605"
     CDS             complement(11410..12822)
                     /gene="uxaC"
                     /locus_tag="IU325_08605"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP5809093.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            13305..14603
                     /locus_tag="IU325_08610"
     CDS             13305..14603
                     /locus_tag="IU325_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5809094.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVDTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            14733..15509
                     /gene="exuR"
                     /locus_tag="IU325_08615"
     CDS             14733..15509
                     /gene="exuR"
                     /locus_tag="IU325_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HAP5809095.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDARTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            15854..16516
                     /gene="yqjA"
                     /locus_tag="IU325_08620"
     CDS             15854..16516
                     /gene="yqjA"
                     /locus_tag="IU325_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HAP5809096.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            16520..16903
                     /gene="mzrA"
                     /locus_tag="IU325_08625"
     CDS             16520..16903
                     /gene="mzrA"
                     /locus_tag="IU325_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HAP5809097.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            17050..17418
                     /gene="yqjC"
                     /locus_tag="IU325_08630"
     CDS             17050..17418
                     /gene="yqjC"
                     /locus_tag="IU325_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HAP5809098.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLTEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            17456..17761
                     /locus_tag="IU325_08635"
     CDS             17456..17761
                     /locus_tag="IU325_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HAP5809099.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            17764..18168
                     /locus_tag="IU325_08640"
     CDS             17764..18168
                     /locus_tag="IU325_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809100.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            18158..18457
                     /locus_tag="IU325_08645"
     CDS             18158..18457
                     /locus_tag="IU325_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809101.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            18553..19035
                     /locus_tag="IU325_08650"
     CDS             18553..19035
                     /locus_tag="IU325_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HAP5809102.1"
                     /translation="MILSIDSNDANTAPLHKKTISSLSGAVESMMKKLEDVGVLVARI
                     LMPILFITAGWGKITGYAGTQQYMEAMGVPGFMLPLVILLEFGGGLAILFGFLTRTTA
                     LFTAGFTLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW
                     "
     gene            19105..20091
                     /locus_tag="IU325_08655"
     CDS             19105..20091
                     /locus_tag="IU325_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAP5809103.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPSALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVVKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            20383..20748
                     /locus_tag="IU325_08660"
     CDS             20383..20748
                     /locus_tag="IU325_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAP5809104.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            20990..21346
                     /locus_tag="IU325_08665"
     CDS             20990..21346
                     /locus_tag="IU325_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAP5809105.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPVIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(21397..22293)
                     /locus_tag="IU325_08670"
     CDS             complement(21397..22293)
                     /locus_tag="IU325_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP5809106.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            22398..23099
                     /locus_tag="IU325_08675"
     CDS             22398..23099
                     /locus_tag="IU325_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAP5809107.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEVLLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            23122..23286
                     /locus_tag="IU325_08680"
     CDS             23122..23286
                     /locus_tag="IU325_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809108.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTANNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(23346..24656)
                     /locus_tag="IU325_08685"
     CDS             complement(23346..24656)
                     /locus_tag="IU325_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HAP5809109.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCDEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVTEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGNWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(24684..26015)
                     /locus_tag="IU325_08690"
     CDS             complement(24684..26015)
                     /locus_tag="IU325_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809110.1"
                     /translation="MEIVSNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKIPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(26291..27655)
                     /gene="tdcG"
                     /locus_tag="IU325_08695"
     CDS             complement(26291..27655)
                     /gene="tdcG"
                     /locus_tag="IU325_08695"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAP5809111.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(27727..28116)
                     /locus_tag="IU325_08700"
     CDS             complement(27727..28116)
                     /locus_tag="IU325_08700"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HAP5809112.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(28130..30424)
                     /gene="tdcE"
                     /locus_tag="IU325_08705"
     CDS             complement(28130..30424)
                     /gene="tdcE"
                     /locus_tag="IU325_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HAP5809113.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(30458..31666)
                     /gene="tdcD"
                     /locus_tag="IU325_08710"
     CDS             complement(30458..31666)
                     /gene="tdcD"
                     /locus_tag="IU325_08710"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HAP5809114.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVCNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNASAEFA"
     gene            complement(31692..33023)
                     /gene="tdcC"
                     /locus_tag="IU325_08715"
     CDS             complement(31692..33023)
                     /gene="tdcC"
                     /locus_tag="IU325_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HAP5809115.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(33045..34034)
                     /gene="tdcB"
                     /locus_tag="IU325_08720"
     CDS             complement(33045..34034)
                     /gene="tdcB"
                     /locus_tag="IU325_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HAP5809116.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
BASE COUNT         8189 a         8913 c         8644 g         8330 t
ORIGIN      
        1 accagccccc atgtcaaaaa catccgaagt ttcaggtctt cacagccctt actgatggat
       61 ggaaatcaga gtgcggaaca tgcgcctggc ccgcaattgc cctccgtcct ggcaagacag
      121 aaagggacca aaaagccccc attatcgacg aagtcattct gaaatggtcg aagatgcccc
      181 cggatgagcg ggctaaagat agcgtgattg actatctccg tagcctgccg tcctgtcagg
      241 acgaccacca cgttacctat taatttttcg aacaccgtac ttgcacaacg agaggtgtcc
      301 atatacagat aagacgcgac aagcgtcgta tccggcattt ccaatgggcg tattcttttt
      361 gaatcccatc acaaaccccg cactcccttt ttcccttttc tctggcgacg gctaaattag
      421 aactcatccg accacataac aattatttta catactggac aattttatga gctacccgtc
      481 gctgttcgcc ccgctggatt taggcttcac cacgttaaaa aaccgagtac tgatgggttc
      541 aatgcacacg ggactggagg aatacccgga cggcgctgaa cggctggcag cgttttacgc
      601 ggaacgcgcc cgtcatggcg tggcgttgat tgtcagcggc ggtattgcac cagatttaac
      661 aggcgttggc atggaaggcg gcgcaatgct caatgacgca agccagatcc cgcaccatcg
      721 caccataacc gaagccgtac atcaggaagg cggcaaaatc gccctgcaaa ttttgcatac
      781 cgggcgctac agctaccaac cgcatctggt cgctccttcc gcattgcagg ctcccatcaa
      841 ccgtttcgtt ccccatgagt taagccatga agagatcctg caactgatcg acaatttcgc
      901 ccgctgcgcg caactggcgc gggaggccgg atacgatggc gtagaagtga tgggttccga
      961 agggtatttg atcaacgagt ttctgaccct gcgcactaac cagcgtagcg accagtgggg
     1021 cggcgattac cctaaccgga tgcgctttgc agttgaagtg gtgcgagcag tgcgcgaacg
     1081 cgtcggcaat gacttcatta ttatctaccg tctgtcgatg ctcgacctgg tagaaggcgg
     1141 cggtactttt gccgaaacgg tagggctggc gcaggccatt gaagcggcgg gcgcgaccat
     1201 tatcaacacc ggcattggct ggcatgaagc acgtattccg accattgcca cgcccgtgcc
     1261 gcgcggtgcc tttagctggg tcacgcgcaa actgaaaggc cacgtctcgc tgccgctggt
     1321 gaccaccaac cggattaacg atccgcaggt tgccgacgac attctctcgc gcggtgatgc
     1381 cgatatggta tcgatggcac gaccgtttct tgctgatgcg gagctgctgt caaaagcgca
     1441 atcggggcga gccgatgaga tcaacacctg tattggctgc aaccaggcct gtctcgatca
     1501 gatcttcgtg ggcaaagtca cctcgtgcct ggtgaatcct cgcgcctgcc acgaaaccaa
     1561 aatgccgatc cttcccgccg tgcagaaaaa aaatctggcg gtggtcggcg ctggtccggc
     1621 tgggctggcg tttgccatta acgcggcggc acgtggtcat caggtaacgt tgtttgatgc
     1681 tcacagtgag atcggcggtc agtttaatat cgccaaacag atccctggca aagaggagtt
     1741 ttacgaaacg ctgcgctatt accgccggat gatcgaagta acaggcgtga cgctgaaact
     1801 caatcacacc gtgacggcgg atcagttaca gtcgttcgat gaaacgatcc tcgctagcgg
     1861 gatcgtaccg cgcattccac ctatcgacgg aatcgatcat ccgaaggtgt tgagttatct
     1921 cgacgtactg cgcgacaaag cgcctgtggg caaaaaggta gcaatcatcg gttgtggcgg
     1981 gattggtttt gatacggcga tgtttttaag tcagccgggt gagtccacca gcaagaacat
     2041 tgccgagttc tgtaatgagt gggggatcga cagcagcctg caacaggctg gtggcttaag
     2101 cccgcaggga atgcagatcc cgcgtagccc acggcagatt gtgatgctcc agcgcaaagc
     2161 cagcaaaccg ggacaggggt taggcaaaac caccggctgg atccatcgca ccaccctgct
     2221 ctcgcgcggc gtgaaaatga tcccaggcgt aagttatcag aagatcgacg atgacgggct
     2281 gcatgtggtg atcaacggcg aaacgcaggt attagcagtg gacaatgtgg tgatctgcgc
     2341 agggcaagag ccaaaccgcg cgctggcgca accgctgatt gatagcggga aaactgtgca
     2401 tttaattggc ggctgcgatg tagctatgga gctggacgca cgacgggcaa ttgcccaggg
     2461 aacacggctg gcgctggaga tttaaatcgt gcattgaagt gccggatgcg acgcttgccg
     2521 cgtcttatcc gacctggggg taatgtgctg aacgtatgcc agataaggcg tttacgcctt
     2581 atctggcagg tagctgccaa tcttaccgac gacgccccag cttcactgct ttcagcacca
     2641 caaatttatt attcgtggcg atggtggtgc agttgccgaa aatcttcttc agtttatgga
     2701 agtaatccag gtgacggttg gcaacgatat acagctcgcc gttgattttc agacagcggc
     2761 gggcgtggtg gaacatctcc caggcgacgt tatcggtcag cgcatgttgc tgatgaaacg
     2821 gcgggttgca gagcacagca ttaaagcgga aaggctcaac gccggagagc gcgttgttga
     2881 tcataaattc gcagcgatcc aatgcctcgg gcatgttggt ttcaacgttc aaacggctgg
     2941 aagcaaccgc catcggcgat tcatcgacaa acaccacttt cgcctgcggg tttttatcaa
     3001 gcagcgtcag accaataacg ccattaccac aaccgagatc gacaatctca ccttcgagat
     3061 tctctggcag atgctgcata aagaagcgcg cgccaatatc cagcccggtg cgggagaaga
     3121 cattcgcatg gttgtggata gtccagtcag taccttccag tttccagcta acggtctgcg
     3181 gcgcatcggc cagcggcggc tcgttgaaag tacagttaat taagcgtgcc ttcttccatg
     3241 ccagcgtggt ggtggtcggg ccgagcactt tttcgaacag ttccagcgtg gaagtgtgga
     3301 tgtcacgggc tttggcaccg gcaataatac gcgtatctgg cgtgaccact ttgcgcagcg
     3361 cacgcagttg ctgttccagc aatgccagtg ttttcggtac tttgatcagc accactcccg
     3421 gctgttgcgg gtagtcagcg gtgctgtcga gaaacttcac gctcgattca tcaatcccgt
     3481 tgaggcgtaa attctcgcgc gtcgccagtt cgctgatgta tgagtcgcca atgctgtatg
     3541 gcttatgttc cgccagcgca caacttaacg caccaaaggc atcattcagg atcaacaccg
     3601 ggccgcggat ttctgtgtcg tccaactgtt gcagcaaata ttcatccgcc gcttcccacg
     3661 cctgtagcgg gttaacgtca tccgtcgccg gaaaacgttg tagtgtcagt gaacggaaac
     3721 cgttgtctaa gtggctcatc ggccctcctg gatgataaaa tttcgacgtt gcccctgaaa
     3781 agggtgcgtg agtataccct ttttctcttt ttcgtgggcg gttatgagca atcttactta
     3841 tcttcagggt tatcccgagc agctactttc ccaggtgcga acgctgatta acgaacagcg
     3901 tctgggcgat gtgctggcaa aacgttatcc gggaacacac gactacgcca ccgataaagc
     3961 cctctggcaa tatacccagg atctgaaaaa tcagtttctg cgcaatgccc cgccgatcac
     4021 taaagtgatg tatgacaaca agatccatgt gctgaaaaac gcgctcgggt tacataccgc
     4081 tgtttcgcgt gtgcagggcg gcaagctgaa agcaaaggcg gagatccgcg tcgctaccgt
     4141 gtttcgcaac gcgccggaac cgtttttgcg catgatcgtg gtgcacgagt tggcgcacct
     4201 gaaggagaaa gagcataaca aagcgttcta tcaattgtgc tgtcatatgg agcagcagta
     4261 ccaccagctt gaattcgaca ctcgtctgtg gctaacgcag ttgtcacttg ggcaggacaa
     4321 aatctgaaaa tgatgtcagg cgttatcttt tcttccttta gcgcctaaaa tcgacctccc
     4381 ccctttcgtt aaggataacg accatgctgc gcgcctttgc ccgccttctt ctccgtattt
     4441 gtttttctcg ccggactctt aagattggct gtttattgtt gcttatagca ggagcgacca
     4501 tttttattgc cgatcgcgtt atggtcaaca ccagcaagca gctgacctgg agcgatgtca
     4561 acgcagttcc ggcgcgtaac gtgggtttat tgctgggagc caggccgggt aatcgctact
     4621 ttacccgacg tattgatacc gcagcagcat tgtaccacgc cggaaaagtg aaatggctgt
     4681 tagtcagtgg cgataacgga cgtaaaaatt acgatgaagc atcaggtatg cagcaggctt
     4741 tgatcgctaa aggcgtaccg gcgaaagtga tcttctgcga ctatgccgga ttctcaacgc
     4801 tggattcggt agtgcgtgcc aaaaaggtgt ttggggaaaa ccatatcacc attatctcgc
     4861 aagaatttca taaccaacgc gccatctggc tggcaaaaca gtacggcatc gacgctatcg
     4921 gttttaacgc gccagacctc aatatgaagc acggttttta tactcaactg cgggagaaac
     4981 tcgctcgcgt cagcgcggtg attgacgcaa aaatcctcca tcgccagccg aaatatctcg
     5041 ggccatcggt catgattggg ccgtttagtg agcatggctg tccggcgaaa gaataatgcg
     5101 tatctgcgca cgtcgaagat gaaaaaggcg tgctacattg acgacagaat ccctttatgg
     5161 agtatccacg cgttatgata cgtttcgctg tgattggtac gaactggatc actcgccagt
     5221 tcgtcgaggc cgcccatgag agcggtaaat acaagttaac cgccgtttat tcccgcagcc
     5281 ttgagcaggc ccagcacttc gccaatgatt tttctgtcga gcatctgttt acctcgctgg
     5341 aagcgatggc agaaagcgat gccattgacg cggtgtatat tgccagcccg aatgccctgc
     5401 acttttccca gacacaactt ttccttagcc acaaaattca tgtgatttgc gagaagccgc
     5461 tggcatcgaa tctggcggaa gtggatgccg ccattgcctg tgcgcgggaa aatcaggtgg
     5521 tgctgtttga ggcatttaaa accgccagcc tgccgaactt tcatctgtta cgccaggcac
     5581 tgccgaaagt cggcaaactg cgtaaagtct ttttcaacta ttgccagtat tcctcgcggt
     5641 atcaacgtta cctggatggt gagaatccca acacctttaa tccggcattc tctaacggtt
     5701 caattatgga tatcggcttt tactgcctgg cgtcggcggt ggcattattc ggtgaaccga
     5761 aaagcgtgca ggcaaccgcc agtttgctgg caagcggtgt ggatgcccac ggcgtggtgg
     5821 tgatggatta cggtgatttc agcgttacgt tgcagcactc aaaagtgagc gattctgtcc
     5881 tggcaagcga gattcagggc gaagcaggat cgctggtgat tgaaaaactg tctgaatgcc
     5941 agaaagtttg ctttgttccg cgtggcagcc agatgcagga tctcacccaa ccgcagcata
     6001 ttaataccat gctctacgaa gcagagctgt ttgctgaact ggtggatgaa catctggtgg
     6061 atcatcctgg gctggcggtc agtcgcatca ccgccaaact gctgaccgag atccgccgcc
     6121 agactggggt gatttttccg gcagatagcg taaaactata attgccaaag taaagcagtg
     6181 taaaaggcat gtaacagacc attgactgac tgaatggtct gtcatacttt gttacctgca
     6241 aaggggagta acttcattgc cggtcgatcg tcattacgat gtgtgaaaaa ccacatccgg
     6301 tcaccgggca acccgaaagg aatacgcaga cgtattcctt ttttgttgta agtgagacct
     6361 tgccggaagg cgaggtctat gcataaaaag cagcggctga cgtcttccga cgttggccgt
     6421 tttttttatg tgtaaggaac ttctatgaat actgtcggca cgccgttgct atggggcgga
     6481 ttcgctgttg ttgtcgccat tatgctggct atcgacctgt tgttgcaggg gcgtcgtggg
     6541 gcacatgcca tgaccatgaa acaggctgcg gcctggtcgc tggtctgggt gacgctgtcg
     6601 ttactgttta acgccgcttt ctggtggtat ctggtgcaaa ccgaaggtcg cgccgttgcc
     6661 gatccacagg cactggcctt tctcacgggt tatctgattg agaaatcgct ggcggtcgat
     6721 aacgtctttg tctggctgat gttgttcagc tatttctctg ttccggcggc attacaacgc
     6781 cgcgtgctgg tgtatggcgt gctcggggcg attgttctgc gtaccatcat gatcttcact
     6841 ggcagctggc tgatttcgca gttcgactgg atcctgtata tcttcggtgc cttcctgttg
     6901 tttaccggcg tgaagatggc gctcgcccat gaagatgaat caggcattgg cgacaagccg
     6961 ctggtgcgct ggctacgcgg tcatttgcgc atgaccgaca ccatcgacaa cgagcatttc
     7021 tttgtgcgta agaatggctt gctgtatgcc acgccgctga tgctggtgct gattctggtg
     7081 gaattgagcg acgtgatttt cgccgtggat agcatcccgg ctatcttcgc cgtgaccacc
     7141 gatccgttta ttgtgctgac ctcaaacctg tttgcgatcc tcggcctgcg tgcgatgtat
     7201 ttcctgctgg cgggcgtagc agaacgtttc tcgatgctca aatatggcct ggcggtgatt
     7261 ctggtgttta tcggtatcaa gatgctgatt gtcgacttct accatattcc aatcgccgtc
     7321 tcgctgggcg tggtgtttgg cattctggtg atgacattta ttatcaacgc ctgggtgaat
     7381 tatcggcatg ataagcagcg gggagaatga ttcgctgtat attaagccat tcctagagat
     7441 aactcattcc agacaaaggt gagctattaa ttatgtaatc ggcttatagc tctttacaaa
     7501 aagaccgcac gggtgttggt ggcgtcaaaa tcgccgaagc gttccctgga ggccggggcg
     7561 acgcgcatgg atgcgcggcg agggcgcatc ttacagggac gttcccttgc gcccgacccg
     7621 gtagccgcaa gggataagct gagggaaccg cgcagcggcg attttgttcg ccagagcctg
     7681 ggggtgcagg gggcggcggc gattggccgc cccctgcgcg ctccctgcgc cagtgacttt
     7741 atatggcata gcacattaaa ggagcgcaac cgttctcata ttccaaatag ttttgcccat
     7801 atgttatcta atcagcctta gtagccagtc cttccctctg tttatgtcaa ataaatgtaa
     7861 ccattaagtt gcaaaatatg acctctcttt aaaatccagc atttttcgct tcccgaagct
     7921 gtaactttcc ttatactcga ccttgcaaac actttgttac atcctgaaag atgcgtcgac
     7981 agaacgcacc agggatgtgc gacaacacaa tgaaaggatc gaaaaatgac tacgcaacgt
     8041 tcaccggggc tattccggcg tctggctcat ggcagcctgg taaaacaaat cctggtcggc
     8101 cttgttctgg ggattcttct ggcatggatc tcaaaacccg cggcagaagc tgttggtctg
     8161 ttaggcactt tgttcgtcgg cgcactgaaa gctgtagcgc caattctggt gctgatgctg
     8221 gtgatggcat ctattgctaa ccaccagcac gggcagaaaa ccaatatccg ccctattttg
     8281 ttcctctatc tgctgggcac cttctctgct gctctggccg cagtaatctt cagttttgcc
     8341 ttcccttcta ccctgcactt gtccagtagc gcgggtgata tttcgccgcc gtctggtatt
     8401 gtggaagtga tgcgcgggct ggtaatgagc atggtttcca accccatcga tgcgctgctg
     8461 aaaggtaact acatcgggat cctggtgtgg gcaattggcc tcggtttcgc actgcgtcac
     8521 ggtaacgaga ccaccaaaaa cctggtcaac gatatgtcga atgccgtcac ctttatggtg
     8581 aaactggtca ttcgcttcgc accgatcggt atttttggtc tggtttcttc taccctggca
     8641 accaccggtt tctccacact gtggggctac gcgcaactgc tggtcgtgct ggttggctgt
     8701 atgttactgg tggcgctggt ggttaaccca ttgctggtgt ggtggaaaat tcgtcgtaac
     8761 ccgttcccgc tggtgctgct gtgcctgcgc gaaagcggcg tgtacgcctt cttcacccgc
     8821 agctctgcgg cgaacattcc ggtgaatatg gcgctgtgtg aaaagctgaa tctggatcgc
     8881 gatacctatt ccgtttctat tccgctgggt gccaccatca atatggcggg cgcagcaatc
     8941 accattaccg tgttgacgct ggctgcggtt aatacgctgg gtattccggt cgatctgccc
     9001 acagcgctgc tgttgagcgt agtggcttct ctgtgtgcct gtggcgcatc cggcgtggcg
     9061 gggggttctc tgctgctgat cccactggcc tgtaatatgt tcggtatttc gaacgatatc
     9121 gccatgcagg tggttgccgt cggctttatc atcggcgtat tgcaggactc ttgcgaaacc
     9181 gcgctgaact cttcaactga cgtgctgttc actgcggcag cttgccaggc ggaagacgat
     9241 cgtctggcaa atagcgccct acgtaattaa tgtttaaccc ctttcgtcca cggcggaagg
     9301 ggttttctcg actttaaacg gatcaattcc ccttttctgc atccgccaga aacgaatgat
     9361 attcaggcca ttcatgagca gaaaactccc ctcaatcatc gtgccgccta tcgaccccgc
     9421 ccagaagttg tgaatcaccc agcaacacgt tgaaaaccac attacgcagc gcatggtcag
     9481 ccctttacag cggaacagcg cccaggtact gacaatcgtg ccgattaccg gcaatagttc
     9541 gacaggatga tggaacttcg cgaggccaat tccgccagtc agcacaataa aaatcgccat
     9601 tacccataag ctgcgcgtgc gtaaagtaat cagcgtacga atggcattaa ggatggcact
     9661 ggcaccggcg ggataggtgc ccagaagaaa aaaatgtacg ccaataacgg cgctatagac
     9721 cgaaagctgc tttttaaagc ggcgttcgtc acggttgaaa aatgttgtga taccaatcag
     9781 aaaggcgatg acacccacgc cctgggccag ccaatacgcg gtcatgataa atccttagca
     9841 ggtctggaaa agcaaacggc gcttcacatt atgaaacgcc gttttttatt aacaactcat
     9901 ttcgacttta tagcgttacg ccgcttttga agatcgccag ttcgcggaag tcgttacgct
     9961 cgttacaggt ttgcttaccg ttggcaaact caacgatggt atcgatgaat tcttccagca
    10021 actgcggcat cgctttaccg tggatcagct gacccgcgtc aaagtcgatc cagtgttttt
    10081 tcttcgccgc cagttcgctg ttggtggcga ttttcaccgt cggtacaaag ccgccgtatg
    10141 gcgtgccgcg accagtactg aacagcacca tatggcagcc cgcacccgcc agggcgctgg
    10201 tcgctacggc atcgttaccc ggcgcactta acaagttcag ccccggtgtt ttcagacgct
    10261 caccgtaacg cagcacgtca accacgacgc tggaacccgc tttctgggta cagccaagtg
    10321 atttgtcttc cagcgtggtg ataccgcccg ctttgttccc cggcgatggg ttttcataga
    10381 tcggctggtc atgggcaata aagtactgct tgaagtcatt gaccatggtg accagttttt
    10441 caaacgttgc ttcgtcgcgg caatggtcca tcagcaactg ctctgcgcca aacatctccg
    10501 gcacttcggt cagtacggta gtaccgccgt tagcaatcac gtagtcagag aaacgcccca
    10561 gcatcgggtt agcagtaata ccagaaagac cgtcagaacc accgcactcc agaccaaact
    10621 tcagttcgct gagtttgcct ggctcgcgtt tatcgttgcg catcacgtta tacagctgat
    10681 gcaaatgctc gatcccggcc tcgatctcat catcctgctg ttggcagatc atgaaatgaa
    10741 cgcgttcagg atcgatatcg cccagcgttt cacggaatgc ggcaacctgg ttgttttcac
    10801 agcccagacc aatcaccagc actgcgcctg cgttcgggtg gcgcaccatg ttttgcagca
    10861 tggtgcgggt attaatgtga tcgtcgccca gctgtgagca gccgtaggtg tggctgaaga
    10921 ggaacacgcc gtcggtacct tcggcgttgt tggtctcttt caggaaacga ttttggatct
    10981 gccgcgcgat gccgttgaca cagcccacgg ttggcaggat ccacagctca ttgcgcaccc
    11041 cgacatcgcc gttagcgcga cgatagatct gcacttcacg atctgccgct tgcgcaggca
    11101 gatcctgaaa atcaggttga tagcgatact gatccagatc gctcagattc gtgcgcgtat
    11161 tgtgggcgtg aacgtgttcc cccgccgcaa tatccgccaa tgcataacca atcggcaggc
    11221 catatttgat gacgttggcc ccttttgcga tatccgttaa cgcaaattta tgtccacgag
    11281 caacatcctg gcgcagcgta acagtctggt tgtcgacact gacttctgtg ccttccgcca
    11341 aatctgctaa agcgaccgcg acgttatcca gcgcatggat cttgatgtat tgcatatcaa
    11401 ccccagacct tagttcagtt caatggcgaa gtagtcacgc gcattgttaa agcaaatatt
    11461 tttcaccatc tcgcccagca ggttgatatc cgccggcgct tcgcccgctt ccacccagcg
    11521 accgatcatc tggcacagaa tgcggcggaa gtattcgtga cgggtgtatg acaggaagct
    11581 acggctgtca gtcagcatac cgacaaagcg gctcaacaga ccgagctgcg ccagttgggt
    11641 catctgacgt tccataccgt ctttctgatc gttaaaccac cagccggaac cgaactgcat
    11701 tttgcccggc ataccttcgc cctggaagtt accgatcatg gtgcccagca cttcgttatc
    11761 gcgcgggttc aggcagtaga gaatggtttt cggtagcagg ttttcttcgt tctgcttgct
    11821 cagcagctta gacagctctt ccgccatcgg acggtcgttg atggagtcaa agccgacatc
    11881 cggccccagc agtttaaact gacgcaggtt gttattacgc agtgcgccaa tgtggtactg
    11941 ctgtacccag ccgcgacgtg catattcggc accaaggaac accagcaccg cagttttgaa
    12001 ctgtgccact tcgtgctcgc tcagggtttc gccagccaga cgacgcgcca gaatgctgtc
    12061 cagttccgct tcgttcgctt cagcaaacat caccacatcc agcgcgtggt cagacacttt
    12121 acagccgtga gcggcgaagt gatccagacg tttggtcagg gcagtttgca gatcagcaaa
    12181 gcggcggatg tcggtatcgg aaacttcgcc cagcttcgcc atgtagtcgt taaaggtcgc
    12241 ctgttcgatg ttgaaggctt tatcaggacg ccagctcggc agcactttaa tggtgaaaga
    12301 gccgtctttg gcgatctctg cgtgatgctc cagagaatcg atcgggtcat cggtggtgcc
    12361 gaccattttc acgttcatct gctgcatgat gccgcgcgcg gagaagttat cctgcgccag
    12421 cagttcatta cattcgttcc agatttcatc ggcagttgac ggagaaagca atttgccagt
    12481 gataccaaac ggacgacgca gttcgaggtg cgtccagtgg tataacgggt tgccgatagt
    12541 gtgcggaaca gtcgccgccc aggcgtcaaa tttttcacgg tcagacgcat caccagtaca
    12601 cagacgctca gccacaccgt tggtacgcat agcgcgccat ttgtagtgat cgcccttcaa
    12661 ccagatgtca tacaggtttt taaaacgata gtcttccgca atctgctgcg gcggcaaatg
    12721 gcaatggtaa tcgaaaatcg gctggtcttt tgcgtagtcg tgatacagac ggcgggcaaa
    12781 ttcggtatct aacaggaaat cttcagtcat aaacggagtc attttcgtct tcctctcaac
    12841 gagtgagcta gcttgcttat ggtgcgatgc tgacaaagtt atcacaccaa tttccagagt
    12901 ccgaagatat tttcgtgaga tagatcaata aacgtagtta aaaaaattac tctcaaagtg
    12961 gtaaatctcg ctgcaggccg cgccagtact ggcctcgctg tcgtcaggta atgtccctac
    13021 aaatattccc acatttgtga tggctctcac cttttaaagt tgtatgacaa gttatctttc
    13081 tgccgtcgca aatcataagt cgacggaatg caaattgccg attcattcat ttgttagatg
    13141 aatcgggtta accggtacgg aagccgaatt agcacgaaac tttcatggca acgttcgggg
    13201 cgtgccggtt ttttttcggt tacccggtcg taactaacat cttcagcctc tggcgggatg
    13261 atggccgcgc ttcctgcgga tataacaaaa cgatgaggtt ttacatgcgt aaaattaaag
    13321 ggttacgttg gtatatgatc gcactggtga cgctcggcac cgtgcttggt tacctgacgc
    13381 gtaacactgt ggcggcagct gcgccaactc tgatggaaga gttaaacatc tccacccaac
    13441 agtattccta tatcatcgca gcctattctg ctgcttatac ggtcatgcaa ccggtagcag
    13501 gttatgtact ggatgtgctg ggtacgaaaa tcggttatgc aatgtttgct gtgctgtggg
    13561 ccgtgttctg tggtgcaacc gcgctggcag gtagctgggg cggcctggct gttgctcgtg
    13621 gtgcggtcgg tgccgcggaa gcagcgatga tcccggcggg tctgaaagcc agctccgaat
    13681 ggttcccggc gaaagagcgt tccatcgcgg taggttactt taacgtaggt tcttctattg
    13741 gtgcgatgat tgcaccgccg ctggtggtat gggcaatcgt aatgcacagc tggcagatgg
    13801 catttatcat ctccggtgcg ttgagcttta tctgggcaat ggcatggctg attttctata
    13861 aacatccgcg cgaccagaag catctgaccg atgaagaacg cgactacatt attaatggtc
    13921 aggaagccca gcaccaggtt gacacagcga agaaaatgtc cgttggtcag atcctgcgta
    13981 accgtcagtt ctggggtatc gcgctgccgc gcttcctggc agaaccggca tggggtactt
    14041 ttaacgcgtg gatcccgctg ttcatgttta aagtttacgg ctttaacctg aaagagatcg
    14101 cgatgtttgc ctggatgccg atgctgtttg ctgacctcgg ttgtatcctc ggtggttacc
    14161 tgccgccgct gttccagcgt tggtttggtg tgaacctgat cgtttcccgt aagatggtcg
    14221 taacgctggg tgctgtgctg atgattggcc cgggtatgat cggtctgttc accaacccgt
    14281 atgtcgcgat tatgctgctg tgtatcggtg gttttgctca ccaggcactg tctggcgcgc
    14341 tgattacgct ctcttctgac gtgtttggtc gtaacgaagt ggcaacggca aacggtttaa
    14401 ccgggatgtc cgcatggctg gcaagtacgc tgtttgcgct ggttgtcggt gcactggctg
    14461 acaccatcgg cttcagcccg ctgttcgcag ttctggcagt attcgacctg ctgggtgcac
    14521 tggttatctg gacggtgttg cagaacaaac cggcgattga ggtggcgcag gaaacacata
    14581 acgatcctgc accgcaacat taaggcttcg tgtaagtttt agtttccctt caaagccgcc
    14641 ttctcaggcg gctttttcat cactgtgagt agagctaaac tcgcaaaagt ggtataacaa
    14701 atatagtctg ccgtatcatg cctggagcgc atatggaaat cactgaacca cgccgtttgt
    14761 atcaacaact tgccgctgat ctgaaagagc gcatcgaaca gggcgtctat ctggtgggtg
    14821 ataaactgcc tgccgaacgc tttattgccg atgaaaaaaa cgtcagccgc accgtggttc
    14881 gtgaagccat catcatgctg gaagttgaag gctatgtcga agtgcgtaaa ggttcaggta
    14941 ttcatgtggt ttccaaccag ccgcgccatc agcaggcggc tgacaataat atggaattcg
    15001 ccaattacgg tccgtttgag ttgcttcagg ctcgccagct catcgaaagt aatattgccg
    15061 aattcgcggc aactcaggta acgaaacagg acatcatgaa actgatggcc atccaggagc
    15121 aggcgcgcgg cgaacaatgc ttccgtgatt ccgagtggga tttgcagttc cacattcagg
    15181 tagccctggc gacgcagaac tccgccctgg cggctatcgt ggaaaaaatg tggacccagc
    15241 gtagtcataa cccgtactgg aaaaaactgc acgaacacat tgatgcccgt accgtcgata
    15301 actggtgtga tgaccacgat caaatcctca aggcgttaat tcgcaaagat cctcatgctg
    15361 ctaagctggc aatgtggcag catctggaaa acaccaaaat catgttattt aacgaaacca
    15421 gcgacgattt cgagttcaat gccgaccgct atctgttcgc agaaaacccg gtagtgcatc
    15481 tcgatacggc caccagcggc agcaaatgaa atttccatcc gacaggcgct gcaatgcgcc
    15541 tgtttgcttc gtcggtaagc gaaaagtata aagtgtcagc ctgtgtaaat cctctcgcca
    15601 ccctcccctg caatcagcaa aatcagactc tacggacatg taattttgat aacgaacaac
    15661 gttgaccttt gttacaatta gattcaattt gaatttatgc ttttgaatgc tttcttatct
    15721 cacgatttaa cagggaatag ttcaggctgt gttgatgtat caaacccgca aaacatacca
    15781 aaacagcaat aacattgcgg tagtgcatct tttaaaacca gcgtggcgtt aaccgattca
    15841 ccaggaataa tgaatggaac ttttgaccca attgctgcaa gccctgtggg cgcaggattt
    15901 tgaaaccctg gccaatccgt cgatgattgg catgttgtat tttgtcttgt ttgtaatttt
    15961 gttccttgaa aacggcttgc ttccggcggc ctttttaccg ggcgacagtt tactggtgtt
    16021 ggtcggcgta ttgattgcga aaggcgcgat gggctatccg caaacgattc tgctgctgac
    16081 cgttgccgcc agcctcggtt gctgggtcag ctatattcag gggcgatggt tgggcaatac
    16141 ccgcactgta caaaactggc tatctcattt acccgcgcat tatcatcaac gcgcacacca
    16201 tctttttcat aaacacggtt tatcggcgct gttaattggt cgctttattg catttgtcag
    16261 aacactgctg ccgacgattg ccggattatc agggctgaat aacgcgcgct ttcagttctt
    16321 caactggatg agcggtctgc tgtgggtatt gatcctgaca actctgggtt acatgctcgg
    16381 caaaacgccg gtatttttaa agtacgagga ccagctgatg tcatgcctga tgctgctccc
    16441 ggtggtgctg ctggtgtttg gcctggcagg ttctctggtc gtgttatgga aaaagaaata
    16501 tggaaatcgg gggtaaggga tgcaaatacc tcgcatgtcg cttcgccagc tagcctggtc
    16561 cggcgctgtt ttacttctgg tcggcacact gttgctggcc tggtccgcgg ttcgccagca
    16621 agagtctacg ctggcgattc gtgccgttca tcaagggaca acgatgccag acggtttttc
    16681 aatctggcat caccttgacg ctcacggcat tcctttcaaa agtatcaccc ccaaaaatga
    16741 cactctgtta attacctttg actccagcga ccagagtgcc gccgcaaaag cggtcctcga
    16801 cagaacattg ccccatggct acatcatcgc gcagcaggac aataatagtc aggctatgca
    16861 gtggctgacc cggttgcggg ataactctca tcgcttcgga taacttcctg ttatccgaaa
    16921 catatcactg actttggtga tttcaccgta actgtctatg attaatgagg cggtggttaa
    16981 cactaccgct tcgttgtctg gacaatcgtt cctttgtaat aggtccgaat cacaatggaa
    17041 ggttcaagaa tgaaataccg catcgcttta gctgtttctc tctttgctct tagtgccggt
    17101 agttatgcca ctaccctgtg tcaggaaaag gagcaaaata tccttaagga gatcagctat
    17161 gccgaaaaac accaaaacca gaatcgtatt gacggtctga ataaagccct gagtgaagtc
    17221 cgggccaact gttcagatag ccagctgcgt gccgatcatc agaagaaaat cgcaaagcag
    17281 aaagatgagg tggcggaacg ccagcaagat ttaaccgagg cgaagcaaaa aggcgatgcc
    17341 gataagattg ccaaacgcga acggaaactg gcagaagcgc aggaagagct gaaaaagctg
    17401 gaagcgcgcg actactaact cacaatagtc actacttact cacctggaga aaactatgtc
    17461 gaaagaacac actacggaac atctgcgtgc tgagttgaaa tccctttccg atacgctgga
    17521 agaggtactt agctcatctg gcgagaagtc gaaagaagag ttgagtaaga ttcgtagcaa
    17581 agcggagcag gcactgaaac agagccgtta tcgcctgggt gaaaccggtg atgccatcgc
    17641 caaacaaacc cgtgtcgcgg cggcgcgtgc cgatgagtat gtgcgtgaaa acccgtggac
    17701 gggcgtgggc attggcgctg caatcggtgt agtgctcggc gttctgctgt cgcgtcgtta
    17761 attatggcgg acactcatca cgcgcaaggg cccggtaaaa gcgttctggg catcgggcag
    17821 cgaattgttt ccatcatggt tgaaatggtg gaaacacgtc tgcggctggc ggtggtggag
    17881 ctggaagagg aaaaagcgaa tctctttcaa cttttactga tgctgggcct gacgatgctt
    17941 ttcgctgcat ttggtcttat gagcctgatg gtgctgatta tttgggcggt tgatccgcaa
    18001 tatcgcctga atgcgatgat tgccaccacc gtggtgttgc tgctactggc actgattggc
    18061 ggtatctgga cgttacgtaa atcgcgtaaa tccaccctgc tgcgccatac acgccatgag
    18121 ctggcaaacg atcggcagct gctcgaggag gagtcccgtg agcagtaaag tcgaacgtga
    18181 acgacgtaag gcgcaactgc tgagccagat ccagcaacaa cggctggatc tttccgccag
    18241 tcgtcgtgaa tggctggagg caacaggcgc ttacgatcgt cgctggaata tgctgctaag
    18301 tctgcgctcc tgggcgctgg taggcagtag cgtgatggcg atctggacga ttcgccatcc
    18361 taatatgctg gtccgctggg ccagacgcgg ttttggcgta tggagcgcct ggcgtctggt
    18421 gaaaacgacc ctcaagcagc aacaacttcg cggttaactt ccctctggcc ggagccattc
    18481 cggccttatc cctcaaattt tttgaagatt tttgacagtt ttccttgcta acattcatca
    18541 ttcaccacgt ttatgattct ctccatcgac agcaacgacg ctaataccgc gcccttgcac
    18601 aaaaaaacaa tcagcagcct gagtggcgca gtggagagta tgatgaaaaa attagaagat
    18661 gttggtgtac tggtagcgcg cattttaatg ccgattctgt ttattaccgc aggctgggga
    18721 aaaattactg gctacgcggg tacccaacaa tatatggaag cgatgggtgt cccaggcttt
    18781 atgctgccgc tggtgattct gcttgagttt ggtggtggtc tagcaatcct gttcggtttc
    18841 ctgactcgca ccacagccct gtttactgcg ggctttacgc tgctgacggc atttttattt
    18901 cacagcaact ttgctgaagg cgtcaactcg ctgatgttca tgaaaaacct gacaatttct
    18961 ggcggattcc tgctgctggc aattaccggt ccgggcgcgt atagcatcga ccgcctgctg
    19021 aataaaaagt ggtaagcacg agctatactt aacgataaaa aacgaggagg aagctcctcg
    19081 tttttgctat tggaggagag aaatatgggt caactgattg acggcgtctg gcatgacacc
    19141 tggtacgata ccaaatctac cggcggtaaa tttcaacgtt cagcttccgc atttcgtaac
    19201 tggctcactg ccgatggcgc gccaggcccc actggcacag gcggttttat cgcagagaaa
    19261 gatcgttatc atctctatgt ttcactcgcc tgcccgtggg cgcaccgcac gctgatcatg
    19321 cgtaaactca aaggactgga accgtttatt tccgtttccg tagtgaaccc gctgatgctg
    19381 gaaaacggct ggacatttga tgacagtttt ccgggtgcaa ccggagatac gctctatcaa
    19441 catgaatttc tgtatcagct ttatctccac gccgatccac actacagcgg acgagttact
    19501 gttcccgtgc tgtgggacaa aaagaaccac accatcgtca gcaacgaatc agcagaaatc
    19561 atccgcatgt tcaataccgc gtttgatgcg ctgggcgcga aagcgggtga ttactaccca
    19621 tcagccctgc aaacgaaaat tgacgaactt aacggctgga tttatgacac cgttaacaat
    19681 ggcgtgtata aagccggttt tgccaccagc cagcaagcct acgacgaggc ggtagtgaaa
    19741 gtgtttgaat cgctggcgcg actggagcag attttaggcc agcatcgtta cctgaccggc
    19801 aaccagctaa ccgaagccga tattcgcctg tggaccacgc tggtgcgttt tgatccggtg
    19861 tatgtcaccc acttcaagtg tgataagcac cgcatcagcg attacctgaa cctgtatggt
    19921 ttcctgcgag atatctacca gatgccgggg attgccgaaa ccgtcaattt cgatcacatc
    19981 cgtaatcatt acttccgcag tcataagacc atcaacccta cggggattat ttcaattggt
    20041 ccgtggcagg atctcgatga accgcatgga cgagatgttc gcttcggtta aaaaaaggca
    20101 ccttacgggg tgcctctgat tattagctaa gtgcgtgtta tttatgtcgg atgcggcgtg
    20161 aacgtcttct ccaacctaca aaacgtttat attcaatatg ttacaatgcc gcgtaggcca
    20221 gataagcgta gcgcatctgg cgattttgcg cttgtcatca gtcttagcgc gccttcacgg
    20281 cactttatta attccttgaa cgaatattta ctgccatttc attcacgtct attcttaatt
    20341 tgctgcttta aaaacaagtg attgagcaaa attgaggcaa aaatggactg gtatctgaaa
    20401 gtactaaaaa attatgtcgg tttccggggt cgtgcgcggc gcaaagagta ctggatgttt
    20461 attctggtca acatcatctt tacgttcgtg ctggggctac tggataaaat gttaggctgg
    20521 caacgtgccg gtggcgaagg catcctgacg acaatctacg gtattctggt gtttttacca
    20581 tggtgggcag ttcagttccg ccgcctgcac gacaccgacc gctcggcgtg gtgggcactg
    20641 ctattcttaa tcccgtttat cggctggctt attatcatcg tctttaactg ccaggcgggt
    20701 acgccaggcg aaaaccgctt tggacctgat ccaaaactgg aaccttaaaa caaatctact
    20761 cgccggatgt ctgactcatc cggcttttat catcatgttg atgaatgaat aatttactaa
    20821 tgtttattta aaatatttca atgtcgttat ataaattaca tataaaaaat aaccatgtat
    20881 ttatcttaaa tatcaaaacc tttataacat ccgaaaaaga aagatacgaa tagcatttac
    20941 acaatacccg gaatagactt tcgtcacttt taattaaagg gatgttttta tgcagtggta
    21001 tttagccgta ttaaaaaatt atgttggttt ctctggtcgc gcacgtcgta aagagtactg
    21061 gatgtttact ctgattaacg ccatcgtcgg cgctattatc aatgtcattc aattgatttt
    21121 aggtttagag tttccattcc tttctctcat ttacctggca gcaacaatca ttccggtcat
    21181 tgctctgtgc gttcgtcgtc tgcacgatac tgaccgttca ggtgcgtggg cgcttctcta
    21241 tctggtaccg gtcattggct ggttagttct gtttgtattt gcctgtttag aaggcaattc
    21301 tggcagcaat cgttacggaa acgatccgaa gtttggttca aattaatctt agaattgggg
    21361 cgatatttcg ccccttttta ttaacaataa caaatattat tttccgctaa aaagtttggg
    21421 aatttcccgc aaacaccagg atttcgcttc ccccatactg tcacgacgcc aggccataat
    21481 aatatcgatc tcgctggtcg attctgggct gacgacacgc aaccgccctt ccgcaatatc
    21541 tttttcgacc atcggatacg gcatcgtcgc cacgccaagc cccgccagta acgcctgacg
    21601 tttatcttca atcgtgctca ccgttaagcg cggctgtttg tccagcagct gtacggtcaa
    21661 caccgggcgc tcacgagcgg tatccgctac cgcaattcca cgatatttca cgcgcgtcac
    21721 ttcagataac ggttctggct cctgatgaat cgggtgatca ggcgcggcaa catagacgtt
    21781 cattaaggta tagagtttgc gcgagttgat ctccgacgag gaacgaaaat gcatatccgg
    21841 cgcgataacg atatccgccc gcccctgctc cagccgttcc catgcccccg ccagcacttc
    21901 ggtgatgatt gccagttggg tattcgcttt tgccgccagt ttgtcaatta acgggaaaaa
    21961 ggcaggtgtc ggtaccagcg cttcggtcac aatggtgaga tgcgtttccc aaccgcgcgc
    22021 gagagcttcc gcatcggtag tcagtttatc tgcggcttcc agcaaaacgc gccctcgctc
    22081 cagcaacatc ctcccgacat tggtgaattt ggtgcgatgg cccgagcggt caaacagcac
    22141 cacatccagc tcttcttcca gtttttgcat ggtgtagcta agtgcggaag gcacgcgtcc
    22201 cagctcatcc gccgccgccg caaaactgcc ccggcgatcg atcgcatcca taacccgtag
    22261 tgcttccagc gttaatgccc tttctttggc catttcgttc tcattcagga aatttgaaca
    22321 taccgggcag aatatctggc taacaatgta gcgtccagcc ccttaccata aaaggaagta
    22381 aagagaggtc aagaattatg attactaccc gaactgccag gcagtgtgga caagcagact
    22441 acggatggtt gcaggcccgg tatacttttt cctttggaca ctacttcgac ccgaaattgt
    22501 taggctatgc ctccctgcgc gtgcttaacc aggaagtgct ggccccaggt gccgcctttc
    22561 agccgcgaac ctatcccaaa gtcgatattt taaatgtgat tctggatgga gaagcagagt
    22621 atcgcgacag cgaaggcaat catgttcagg ccagcgctgg tgaggtgttg ctgctctcta
    22681 cccagccggg tgtcagctat agcgaacaca atctcagcaa agacaaaccg ttaacgcgaa
    22741 tgcagctttg gctggatgcc tgcccgcagc gagagaatcc actgattcaa aagctggcgc
    22801 ttaatatgga caagcagcaa ttaatcgcct cgccagaggg tgcgatggga agcctgcaat
    22861 tgcgccagca agtgtggctg catcatattg tgctcgacaa aggcgaaagt gcgaatttcc
    22921 agttgcatgg gccacgcgcg tatttgcaat cgattcacgg gaaatttcat gcacttacgc
    22981 atcatgaaga gaaagcggcg ctgacctgcg gtgatggggc gtttattcgt gacgaggcta
    23041 acattacgct ggttgccgat tccccactgc gcgctttgct gatagatttg cctgtctagt
    23101 tgttttaaca gggagatgat gatgagtaaa aaatcggcca aaaagcgtca gccagtgaag
    23161 cccgtggtgg cgaaagaacc tgctcgcacc gccaataatt ttggctatga agagatgttg
    23221 agcgagctgg aagccatcgt cgcggatgct gaaacgcgtt tagccgagga tgaagctact
    23281 gcgtaaagac aggtgccgga tgcggcgtaa acgccttatc cggcacgata cccaaaacct
    23341 ggcgattatc tggccttgct cgccataatc tcgataatct gccgatccgt ttgctgcatc
    23401 gaatggcttg ctaacgcaca caggttggca atcgactgct caacatcatg cgccacaatc
    23461 ccttcattgc cggtcacggc ggtatcatcc agcgccatta acaccgcttt ccacgcagcc
    23521 gaagcactgg tcgaaacctt catcgcgcag ctgttcgacg ctccatcgca aatcatgccg
    23581 ctgacatcgc cgatcatact gctgatcgcc atcgaaatgg tttcataacg cccatccacc
    23641 agccatgcca tcccggcggc ggcccccatt gctgcggtcg ttgcggcaca aagcgcagac
    23701 aaacgcggta actggttatg gatgtaaatc gcgctcaaat gcgaaagcat cagcgcacgc
    23761 gccagccgtt catcatccgc tccgaagtgt tctgctacca ccaccacagg cattgttgcg
    23821 gtgatcccct ggttacccga gccggagtta ctcattgccg gaagcgtagc gccgcccata
    23881 cgcgcatcgg atgccgcgct ggtacgaatc acaatggatg aagagagatc tttcgccagc
    23941 aaaccgcgcg cgcactgttt ttccagcgtc gcaccaatat gcagccccca gttaccgctc
    24001 aaaccttcct gtgataacgc gcaatttaac ttcgcagaat cgagaataaa gcggatcgcc
    24061 gcaaacggga cttcattgac gaacttcagg atctcagcca gcgtcgtcct ggaaagcacc
    24121 gtcagcggcg actcttgctc gccctctgtc acacacgcct gctgagtaaa caccacacca
    24181 ttgtgcgtct caatatgcac aatgttggta tgcccgccga cgatggtgac acacgcccac
    24241 ttctcaccgt tccagacttt ggcgcgtgag aagaggattt catcgcaagg ttcctggatc
    24301 ttaactgaga ctttccccgc cgccagcagt gctttggcat cggcaattgc ctgcgcagtt
    24361 gcgtctttca gcacttccag cccggcgtta gcatttccac ctaacgcccc aagcgccgcc
    24421 gcaatcggca gccccaccat tcctgtaccg ggaacggtga cacccagacc gttcttcatc
    24481 agatttggcg aaacccaggc ttctacacgt tcaaccggac cttccagttc agccgcagca
    24541 accgccgccg ccagcgccag tgaaatcggt tcagtacacc ccagcgccgg ttttacctcc
    24601 tcctgaacgg cgaggatgta acgctgccat aacggattta aagtcgaatc aaacatatta
    24661 aaaaccttaa aatttcaggt aaatcaagaa aatgccagga acggagaaac acaaagcaac
    24721 aaaccagtga cgataatcag gtacagagac atccctttgt atttgtgcaa tgccggtact
    24781 ttgtaaacca gccacgccgg gatcaggcac cctaccatgc cgaaaatcgg gctacagata
    24841 gaggtgaagc tcaacaccgg ggcgttcagt acgatggcgc tccaggccag caaaatggcg
    24901 aaaatcatga tgccgcgctg aacgagattt tcgttaatct tctcggcagg tatcttgcga
    24961 cgcaggatgt tcattacgat cccttgcgtt gcttcgcgaa agcctaaata gacgccaaag
    25021 aatgcagtca ttacagcaaa gatattgaga atgacgctga ccactttcac ccacgctgcg
    25081 ccgtcaccgc taataaactg tgcggcaatc gccagcgcgg aaatattctg ctcgtaggct
    25141 tttaccgctt cgtcatgtcc catcgccagc gtgaacgaca cggcgtagaa aaagacggtg
    25201 acaaacaaaa tgccaaacgc gatattcatt gcccgcaatg ctttatgccg cgctacttca
    25261 atcgattttt cccgcgagcg ataagaaatc accatcgggc ttaacgtctg gataaacaga
    25321 atcgacgtca gggtaaacgg cagcgtaata atggcgtttt tcaccagcag ccccagcggc
    25381 ggtagcgaac cgacgttata cagatgccac attcccacca tcgacacgcc cagcgccgcg
    25441 accaccagca gcttggtcag caccatcccc gtcgaaattt tgaataacaa tttctcgccg
    25501 cgtgaggaga tcgccaccag aatgcaaatc agtaccagac cataaaaggg actgtctgac
    25561 agcaaccctt ccgtcacgcc gaaggtatgc aggtaggaag cactatcgtt ggtgatggcg
    25621 gtggaataga cgaacatcca aatcaccagc attacgaaat agagtgcacc taacaggatg
    25681 ccccagtttt tacctaaata accgctaatg acgctcgggt aatctttaca ttctggtgat
    25741 tctgccagcg tattaataaa caaccgctga aacagataca ttgccgggta accaatcacc
    25801 gatgagagca aaaataccca caatcccatc aaaccgacct gcaccgggag aaaaacaatc
    25861 cccgcgccaa tcgccatccc gatactcata atcacccagc cggtgtcagt actgtcgaat
    25921 ttgatcgctt ctcgccactc gctctcactc attccagcac gacccgccgg ggtcgaagcg
    25981 tctgcaatga cgcctttatt cgatacaatt tccataattt ctcgctcaat attttgtagg
    26041 gcttattatt tttttccgag ccgcatcaag gcgatatgcg gtctacgttt ttgcaggcga
    26101 gttattagaa taaagaaatg atacgcgcga ggtcagagaa aatcttcaca atcaagactt
    26161 tgaaataacg gagatggata agaattttct actaaattaa tcacaagaga atattcattc
    26221 tctatttgcg gcagatcaca aaaaaaaagg tgcacatttg tgcacccaag gatgaaagct
    26281 gacagcaatg tcagccgcag accactttaa tggccagtcc tccgcgtgat gtttcgcggt
    26341 atttatcgtt catatctttg ccggtttcat acatcgtctc gatcacttta tcgagtgaaa
    26401 cacgcggtgc cgaggtgcgg cgcatcgcca tccgcgcggc gtttactgct ttcacggcat
    26461 taatggcatt acgttcaatg cacgggattt gtacctgtcc ggcaaccgga tcgcaggtca
    26521 gcccaaggtt atgctccatc gcgatttccg ccgcattgca tacctgcgcc ggactgccgc
    26581 ccaggagttc agttaaccct gccgccgcca ttgaacaggc cacgccaatt tccccctgac
    26641 agccgacttc cgcgccggag atggaggcgt tcattttata cagcgcgcca atagcccccg
    26701 cggccagaaa atagcgggca attgaccgct cgtttaccgg acgacggaac ttatcgtaat
    26761 aagccagcac tgccggaata atgccgcacg caccgttagt cggtgccgtt accacgcgcc
    26821 cgccagctgc gttttcttca ctaaccgcca gcgcgtacat attgatccag tcgatgacat
    26881 tcatcggatc gttagagatg ttatcgctgg aaaccagctg acgacgcagc gctacggcac
    26941 gacgcggcac attgagcgga ccaggcagca cgccttcagt gttcatcccg cgttcaatac
    27001 cgtcatgcat cacttgccag atacgggcaa aaccggcgtc aatttccgct ttgctacgca
    27061 gcgccagctc gttgtgcatc atcagaccag atatagacag gccgttgtaa tcacacattt
    27121 tcagcagttc acctgctgag tggaaatcgt aaggtacgga cgtttcgaca tcgtgcgaca
    27181 ggccgaagtg ttcttcttcg acaataaacc cgccgccgac agagtaatag gttttactta
    27241 atagcgcttc ctgtcccttc caggcagtga tccgcattcc gttctcatgg cgaggcaaca
    27301 tttcgggatg gaagataatg ttctttgcta caggaaaatc aacaatatgc gcaccagatg
    27361 ccactggcag ccgcccgctg cgcgttacta actctataaa tgcagggatc tcatcaatga
    27421 caacatcctg cggactgttt cctgccagtc ccatgataat ggcgacatcc gtggcatggc
    27481 ctttgcccgt cagtgacaac gacccgtaca gatcgaccac aatatggctc gtcgcggtta
    27541 ataagccgct actttccagc cgatcaataa aactttttcc ggcattcatt ggccccacgg
    27601 tatgcgaact ggagggacca atcccaattt tgaaaatatc gaatgcacta atcatatcca
    27661 caccctcgga ttgccgttca gtgaagtgga gcggaacgac cttgcgaccg tcccgctcac
    27721 gaggctttac gcactacgta ctgcgatggc ttcaatttcc agcttcacat ctttcggcaa
    27781 acgcgcgacc tgtacacagc tccgggtcgg ataggtcgcc tgatgctcat cgaagaactg
    27841 cttatagact tcgttgatgg tggcaaaatc attcagatcg gtgatgaaca cggtcatctt
    27901 gatgatatcg cccacgctca gcccggcagc aaccacgatc gctttgacgt tttcgaggct
    27961 taaacgcgcc tgatcttgca catcagccgg gatctcaccg gtttgtgggc aaaccggaat
    28021 ttgcccggag gtgaagacca tgctgcctaa atcaacgccc tgaacataag ggccgattgc
    28081 gcctggggca cgttgcgttt cgataatctt tttcatacat cctccggcgt cagagcgcct
    28141 gggtaaaagt acgtgaaata acatcctgtt gctgttcacg ggtcagtgcg ttgaagcgca
    28201 cggcgtagcc agagacacgg attgtcaggt taggatattt ttccgggtgc tcgatggcat
    28261 ccagcagcat ttcccgattc attacgttga cgttgaggtg ttgaccgcct tcgacatccg
    28321 cttcgtggtg gaaataccca tccagcaggc cgacaaggtt ggttttacgt actggatctt
    28381 ctttgcccag cgccgcaggc acaatcgaga aggtgtacga gatcccgtct ttggcgtagg
    28441 tgaacggcag tttcgccacc gacgtcaatg aggccacggc acctttgcga tcacgaccat
    28501 gcatcgggtt agcgcccggc gcgaacggtg ttccggcgcg acgaccgtcc ggcgtattac
    28561 cggttttctg gccgtacacc acgttagaag tgatagtcag aatcgactgg gtagggacgg
    28621 cgttgcgata ggttggcagc gctttaattt tcttcataaa gcgttcaacc agatcgcagg
    28681 caatgctgtc tacgcgctcg tcgttgttac cgtactgcgg atattcgccg tcgatttcaa
    28741 agtccaccgc caggccgttt tcgtcacgga ttggtttcac gcgggcatat ttgatggcag
    28801 acagggagtc cgtcgccacc gacaggcccg cgatgccgca tgccatagtg cgatagacat
    28861 cacgatcgtg cagcgccatc agcgaagctt cgtagctgta cttgtcgtgc atgtagtgaa
    28921 tgatattcag cgcactgatg tactgcaccg ccagccagtc catgaagtga tcgagactgt
    28981 ccatcacttt gtcgtaatcc agcacgtcgt ccatcaacgg tgctgttttc ggcccgacct
    29041 gaatcttcag cttctcgtcc accccgccgt taattgcgta cagcagtgtt ttcgccaggt
    29101 tagcgcgtgc accaaagaac tgcatttgct taccaatcac cattgggctg acgcagcagg
    29161 caatcgcgta atcgtcgctg ttgaagtcag tacgcatcag atcgtcgttt tcatactgca
    29221 aggaagaggt gacgatcgac acctgcgcgg catatttttt gaaggcaatc ggtaattctt
    29281 ccgaccaaag aatggtcagg ttaggttccg gtgcaggccc catagtgtgc agggtgtgca
    29341 aatagcggaa ggagtttttg gtcaccagcg tacgaccgtc cagccccatc ccgccgatca
    29401 cttccgtcgc ccagattggg tcgccggaga acagcgaatc aaattccggt gtacgcaaga
    29461 agcgcaccat acggatcttc ataatgaagt ggtcgatcag ttcctgtgcc tgctgctcat
    29521 tgagtacgcc agctttaaag tcgcgctcaa tgtagatatc gaggaacgat gccgtgcgac
    29581 ccagcgacat cgcgccgccg ttctgtgatt tcactgccgc caggtaagcg aagtagagcc
    29641 actgcaccgc ttcctgcgca ttctgcgccg ggcgagagat atcaaagcca tatttcgccg
    29701 ccatttcctg aatctgcaac agcgcatgac gatgctctgc cagctcctca cgcagacgga
    29761 tggtggcttc cagatcctcg cctttttcca gacgagactg gagatcggca aattgcagtt
    29821 cgcgttcacg caccagataa ctgatgccat acagcgctac gcggcgatag tcaccgataa
    29881 tgcgcccacg accatagcca tctggtaaac cggtcagcac gccagattta cggcagcgca
    29941 gcatatccgg tgagtaaaca tcaaatacgc cctggttatg ggttttacgc agatcggtaa
    30001 acagatattc aaattcactg tccatttcac gaccataggc gtggaatgaa cttttaatca
    30061 tattaatgcc accgaacggg tgtaacgcac gtttcaacgg cgcatccgtt tgcaggccaa
    30121 caattttttc cagcggctgg ttaatatatc ccgcatcatg agcggtaatg gtggtggcaa
    30181 tattggtatc gaaatcaacc ggcgcgtggg ttgcgttttc aatacgaatg ccttccatca
    30241 ccttttccca caattccgtg gtggctggcg tcgcttcagc gaggaaagat tcatcgcctt
    30301 catacggtgt atagttatgt tgaataaaat cgcggacatt aatttcgttt ttccagtccg
    30361 tacctttaaa gccaagccat gcgtcggcgt acagcttatc gctggtatca atatctacct
    30421 tcatgaaaaa taatctctct acaatacttc aactaaatta tgcaaattct gcggacgcgt
    30481 tcactttgcc taaatgaatg gcatccaaag caatcatttt ttcttcgttg gttgggataa
    30541 cggcgcaaat gacacgggca ttttcactgg aaacaattcg ctctccacag gagttagagc
    30601 gattattcat ttctgtatca atctctacgc ctaatacagc caaatgttcc atgaccagac
    30661 gacgaattaa acttgaattt tctcctattc cgccggtgaa tataattcca tccaggcgac
    30721 gtaatgaggc tgcgtgtccg gcaatatgac gggcaattcg gtgaacaaag gttttaattg
    30781 ccagttgcgc gcgttcgtga ccttcatgcc aggctttttc cagaacacgt aaatccgaag
    30841 aaagaccgga aatacctaat aatcccgact ctttattcac tacgcgttcc aggtcaccca
    30901 ggctctggtt ggtttggctg gcgacccagg acatcgcacc aaagtcgaca tcaccactgc
    30961 gggtacccat catcaagcct tccagcggcg tcattcccat tgaagtgtca acactctgac
    31021 cgttgcaaac tgcgcagatt gacgcgccat tgccaagatg cgccacaacc aggccggagt
    31081 catcttccgc cagattcagc aaagaatgcg cgcgctggga aacatagcgg tgcgacgtgc
    31141 cgtggaaacc ataacggcgt acacctaact cttcataata tttccacggc aggccgtata
    31201 aataagcttc cggagccatc gtctggtgga aactggtatc aaataccgcc acctgagtta
    31261 cgccaggaaa taattgctgc gccgattcaa taccacttaa attggcgtaa ttatgcaggg
    31321 gtgccagtgg agaaacgcga cggatattat caatgacttc atcggtaata atggcggact
    31381 cggtaaaaat actgccgccg tgagcgatgc ggtggccaat taaggccaca ctgtcattta
    31441 aattccgttt ttccagttca aatgcaattg ccttcaatgc accttcgtag ctgtggtgag
    31501 ccagcggtgc tggctctccc ccatttacgg ataagaatgc attttccgag ttaataccgt
    31561 cggcaatacc tgacattaat acttcacagt cgctggcgtc gagtacggaa aacttaatcg
    31621 aagacgaacc acagttaata accaaaacaa ccggaaattc attcatctct tttctcatcc
    31681 tgagttacgg attaaaacag tttgtatacg atgttcagga tggtcagcag accaatcacg
    31741 gtaacaaaca cgttatccag acgaccacgg tatttcgcca gagacggcgc tttacggatg
    31801 gcatacatcg gcaacaggca cagcagggat gcgataatcg gtgcgcccat ggcttcaatc
    31861 aggtcgagga tgttcgggtt ggcgtaggca acaacccagg tggagcccat gatgaagatc
    31921 atgctgagag tattcagttt acccagcgac actttggttt tgtcaccttt ataaccgaac
    31981 ttcagaatca ggccattcaa gccttccagt gtccccagat agtgaccgaa gaaagatttg
    32041 aagatagcca cgagtgcgat gatggaagcc gcatattcca gtgtaatcgc gaacgttgtt
    32101 ttggtaccgg tcatggacgc aaagtggtta gccagataag aaagcactgg aatattctgc
    32161 gctttggctt ccgccatgtt ggccggagac agcgtaaaca ggcagctaaa ggcaaagaac
    32221 atcaccactg caaccatcag catgctggca cgagaaatga tttgggaaca tttacgttcg
    32281 gtgaagtcgc gaccgaagtc tttctcatac tcttcacgtt tagaaaccac gaaggaagag
    32341 acgattggcg agaagttaaa ggagaaaacc atgatggaaa tccccagcca gacagtgatc
    32401 aggataccgt catgaccggt taacgacagc gaaccgaggt caacctggtc gataactgca
    32461 gagttccagt aagggatcag cgacagagaa atcagcacca ggctggcgat aaacggccat
    32521 accaggtagc tcatcacttt aaccatcaga tccttaccaa accagatgac gaaagccatc
    32581 agcagcaaca ggaacagcgc cacaaagccg cgattcagcg gtgcaaagcc gagctggttt
    32641 tcccagaacg tcataaaggt attggtaata gtaacgccat aaatccacag cagtgggcaa
    32701 atcgcgaaga agtacaggaa cgtgataacc acgccgccag ttttaccaaa atgctcttcc
    32761 accgtttccg taatgttgcc ggaagggtta gagccagaaa gacacagacg cgccagcgcc
    32821 cggtggcaat aaaatgcgat ggggtatgct aataccaaca tcagaagaat cgggatcagt
    32881 ccgccaaaac ctgcgcggat agggaagaac agcaccccgg cgccgattgc cgtaccaaac
    32941 aagcctaacg tccatgtggt atctgattta cgccatgacg attgttttgt ctggctggat
    33001 acaatgctat ctgaagtact catatcctat cctcaacgaa ttaattaagc gtcaacgaaa
    33061 ccggtgattt gagagacgcg agaaagatcg atattgccgc cggaaataat actgacggtt
    33121 tttctgtttt gaatatattg gtctaattta ccgcttaata atgcagcaca tgccagagcg
    33181 cctgcgcctt cggtgacgac tttattgcgc tgaattaagg caatcatact gtttctgatt
    33241 tcgtcttcgc tgaccagcac gatgtcatcg actaattcac gaacgatttc gtaagttaaa
    33301 ttacccgggc gggagacatc acaaccatcc gccagggtgc cggtagttcg gtgcgtggtt
    33361 atttctccgg agtggaaaga agccgccatg ccgtgaacgt tttctgactg tacgccaata
    33421 acacgaatgg tcgggttaat agattttatt gccaccgcaa taccagcaat taaaccgcca
    33481 ccaccaattg gcacaatcac attatcgaca tcatagagat cttccataat ttccaggcca
    33541 atcgttccct ggccagcaat cactttcgga tcatcataag gtgggataaa aatacggcct
    33601 tccatttcga caatttcgct cactttagcg atagtgtcgt tgaagttatc accatgcaga
    33661 acgacttctg cagagtagtc gcacgttgcc gctactttgg atttcggcgc gccttttggc
    33721 atcaccactt taccgtcgat acccagcatc gcgcaggaga gggaaacccc ttgcgcatgg
    33781 ttgcccgcag agcaggccac tacaccttta cgtttttccg catcggtcag tgaacttaat
    33841 ttattaaatg cgccacgaat tttaaatgaa ccggtacgct gcatgttttc gaatttgagg
    33901 aatatttcac ctttgcaacg ttcactaaaa tagttggagc gaggcatgcc tgttttataa
    33961 attcgcccag ccagtcgttg tttcgcttca atgatgtcat caatagcaac cgggagatcg
    34021 tatgtaatat gcattataaa acctcttagc ctgaattaaa tatgtaggta accgta
//
LOCUS       DACBVI010000038        33521 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.038, whole genome shotgun sequence.
ACCESSION   DACBVI010000038 DACBVI010000000
VERSION     DACBVI010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.038"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(83..559)
                     /gene="radC"
                     /locus_tag="IU325_08725"
     CDS             complement(83..559)
                     /gene="radC"
                     /locus_tag="IU325_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAP5809117.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(575..1060)
                     /locus_tag="IU325_08730"
     CDS             complement(575..1060)
                     /locus_tag="IU325_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAP5809118.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(1115..1933)
                     /locus_tag="IU325_08735"
     CDS             complement(1115..1933)
                     /locus_tag="IU325_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HAP5809119.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVEVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            complement(2033..2266)
                     /locus_tag="IU325_08740"
     CDS             complement(2033..2266)
                     /locus_tag="IU325_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HAP5809120.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            complement(2345..2800)
                     /locus_tag="IU325_08745"
     CDS             complement(2345..2800)
                     /locus_tag="IU325_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809121.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFNPQDYHPLLITVSGKNVN"
     gene            complement(2876..5392)
                     /locus_tag="IU325_08750"
     CDS             complement(2876..5392)
                     /locus_tag="IU325_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809122.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLMLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYNSFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPT
                     VRLALQKGTGAGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNENLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGNMVNRLEEALPPELPA"
     gene            complement(5513..8635)
                     /gene="ag43"
                     /locus_tag="IU325_08755"
     CDS             complement(5513..8635)
                     /gene="ag43"
                     /locus_tag="IU325_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HAP5809123.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKGAGVAVALSLAA
                     VTSVPALAADSIVQAGETVNGGTLENHDNQIVLGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGVATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGIARDTLIHVGGDQSVHGRALNTTLNGGYQYVHKDGLALNTVINEGGW
                     QVVKAGGAVGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVTGINRLGAFSV
                     VEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTL
                     TQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFVQGNRL
                     QAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRIVAGSRSHQTGVNG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLMRTEVAGMSVTAGVY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLWADIVALGTRHSMKAST
                     DNNDFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGKDNAGYVKFGHG
                     SAQHVRAGFRLGSHNDMTFSEGTSSRAPLRDSAKHSVSELPVNWWVQPSVIRTFSSRG
                     DMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGSSAEGY
                     NGQATLNVTF"
     gene            complement(9006..9878)
                     /locus_tag="IU325_08760"
     CDS             complement(9006..9878)
                     /locus_tag="IU325_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HAP5809124.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQRVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDS
                     VVSVARAVWNWIFF"
     gene            11109..12593
                     /locus_tag="IU325_08765"
     CDS             11109..12593
                     /locus_tag="IU325_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HAP5809125.1"
                     /translation="MSFGQPCDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSG
                     PLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNALLNILES
                     QFRLSVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
     gene            complement(12915..13517)
                     /locus_tag="IU325_08770"
     CDS             complement(12915..13517)
                     /locus_tag="IU325_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809126.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLAYRTTVDDKRT"
     gene            complement(13604..13768)
                     /locus_tag="IU325_08775"
     CDS             complement(13604..13768)
                     /locus_tag="IU325_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HAP5809127.1"
                     /translation="MAFITQLTGLTDKWFYKLIKDGDFPAPIKMGRSSRWLKSEVEAW
                     LQARIAQSRP"
     gene            13807..15020
                     /locus_tag="IU325_08780"
     CDS             join(13807..14101,14101..15020)
                     /locus_tag="IU325_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP5809128.1"
                     /translation="MIFSPQHKTGDLMNKKTKRTFTPEFRLECAQLIVDKGYSYRQAS
                     EAMNVGSTTLESWVRQLRRERQGITPSATPITPDQQRIRELEKQVRRLEEQNTIFKKG
                     YRALNVRLAERFTIAARLSDSHTVVSLCSALEIHRSSYRYWRKRRDTVNPARVRLCSE
                     IRRAWNQSRGSAGARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQE
                     HTSLPNLLERQFAVPEPDRVWCGDITYLWAGNRWCYLAVVMDLFARRVIGWSLSAHAD
                     TALISSALRMAYETRGQPRDVVFHSDQGSQYTGLKYQQLLWRCRINQSVSRRGNCWDN
                     SPMERFFRSLKTEWVPTNGYAGKDEARQQINDYILNYYNSVRPHHYNGGLTPEESENR
                     YHFYCKTVANIT"
     gene            complement(15410..15610)
                     /locus_tag="IU325_08785"
     CDS             complement(15410..15610)
                     /locus_tag="IU325_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HAP5809129.1"
                     /translation="MVIRKKKCRDCGNAITHNTVYCPYCGSVDPFGYYRNTDRIVTIL
                     LALIIVVLLTTVSVSVYILCSW"
     gene            15810..16379
                     /locus_tag="IU325_08790"
     CDS             15810..16379
                     /locus_tag="IU325_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HAP5809130.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRDPGSLAGMIKQAWCSALGVDAGCYATLVHFPVRPAVWLERDDETGFQ
                     QVLERAGYLAKEHTKARGTGERNFGCSRG"
     gene            16546..16944
                     /locus_tag="IU325_08795"
     CDS             16546..16944
                     /locus_tag="IU325_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809131.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGGFSLPRTAQQLSCT"
     gene            complement(16948..17916)
                     /locus_tag="IU325_08800"
                     /pseudo
     CDS             complement(16948..17916)
                     /locus_tag="IU325_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847259.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            18102..18506
                     /locus_tag="IU325_08805"
     CDS             18102..18506
                     /locus_tag="IU325_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809132.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKNNRQDVLCCVSCGCPLTLKR
                     GNDGQPPWFEHDQMTVAAKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRERGAAGGQQEG"
     gene            18797..20415
                     /locus_tag="IU325_08810"
                     /pseudo
     CDS             18797..20415
                     /locus_tag="IU325_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            20459..20787
                     /locus_tag="IU325_08815"
                     /pseudo
     CDS             20459..20787
                     /locus_tag="IU325_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420459.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltranspeptidase"
     gene            complement(20827..21799)
                     /locus_tag="IU325_08820"
                     /pseudo
     CDS             complement(20827..21799)
                     /locus_tag="IU325_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
     gene            complement(21802..22767)
                     /locus_tag="IU325_08825"
                     /pseudo
     CDS             complement(21802..22767)
                     /locus_tag="IU325_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462450.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22824..23582)
                     /locus_tag="IU325_08830"
     CDS             complement(22824..23582)
                     /locus_tag="IU325_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HAP5809133.1"
                     /translation="MNNTKKSLKVLFIGESWHIHMIHSKGYDSFTSSKYEEGATWLLQ
                     CLKNSQVDVTYMPAHTVQIAFPEDVAQLEQYDAIVISDIGSNTFLLQNDTFYQLRIKP
                     NALELIKEYVNNGGGLLMIGGYLSFMGIEAKANYKNTVLADVLPVTMLDGDDRVEKPE
                     GVIAQPSQPEHPVIKGFSEYPFFLGYNRAIAKENAEVVLTINNAPLLVFGNYHNGIIA
                     CFMSDCSPHWGTQQFMSWPFYTALWVNILTHIAR"
     gene            complement(23596..24806)
                     /locus_tag="IU325_08835"
                     /pseudo
     CDS             complement(23596..24806)
                     /locus_tag="IU325_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462448.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
     gene            complement(25002..25344)
                     /locus_tag="IU325_08840"
                     /pseudo
     CDS             complement(25002..25344)
                     /locus_tag="IU325_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            26861..27406
                     /gene="cobU"
                     /locus_tag="IU325_08845"
     CDS             26861..27406
                     /gene="cobU"
                     /locus_tag="IU325_08845"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HAP5809134.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            27403..28146
                     /gene="cobS"
                     /locus_tag="IU325_08850"
     CDS             27403..28146
                     /gene="cobS"
                     /locus_tag="IU325_08850"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HAP5809135.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLVLMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGESILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            28158..29237
                     /gene="cobT"
                     /locus_tag="IU325_08855"
     CDS             28158..29237
                     /gene="cobT"
                     /locus_tag="IU325_08855"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HAP5809136.1"
                     /translation="MQILADLLNTIPAIDSTAMSRAQRHIDGLLKPVGSLGKLEVLAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AEQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     QELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            29299..30234
                     /gene="ldtA"
                     /locus_tag="IU325_08860"
     CDS             29299..30234
                     /gene="ldtA"
                     /locus_tag="IU325_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAP5809137.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPDTVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(30290..30365)
                     /locus_tag="IU325_08865"
     tRNA            complement(30290..30365)
                     /locus_tag="IU325_08865"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30330..30332),aa:Asn,seq:gtt)
     gene            30692..31609
                     /gene="nac"
                     /locus_tag="IU325_08870"
     CDS             30692..31609
                     /gene="nac"
                     /locus_tag="IU325_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator"
                     /protein_id="HAP5809138.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            31711..32661
                     /gene="cbl"
                     /locus_tag="IU325_08875"
     CDS             31711..32661
                     /gene="cbl"
                     /locus_tag="IU325_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HAP5809139.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGIVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(32699..32774)
                     /locus_tag="IU325_08880"
     tRNA            complement(32699..32774)
                     /locus_tag="IU325_08880"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32739..32741),aa:Asn,seq:gtt)
     gene            32779..>33521
                     /locus_tag="IU325_08885"
     CDS             32779..>33521
                     /locus_tag="IU325_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN/FAD transporter"
                     /protein_id="HAP5809140.1"
                     /translation="MLRHILTAKNLLSNPIFNFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLL"
BASE COUNT         8241 a         8055 c         8624 g         8601 t
ORIGIN      
        1 ccacgagtga taattttcat aatgggatac ctctgaagac agaagataaa agtgaaaaca
       61 ggtgtgatgt ggttgtgacg ggttaaagca gaccatgttc ggcaaaggag aaaacctggt
      121 tgccaccgac tatcagatgg tccggcaccc ggatatccac caggcccagt gcctgtacca
      181 gacgttcggt gataagccgg tctgccttac tgggtgtgac ttcaccggac gggtgattgt
      241 gcgccagcac cacggcagcg gcattgtggt acagggcgcg tttaatcact tcccggggat
      301 ggacttccgt gcggttgatg gtgccggtga agagggtttc accggcaatc agctgattct
      361 ggttattcag atacagtacc cggaattctt cacgctccag tcccgccatg ttcagaatca
      421 gccattcccg tgccgcacgg gtggaggtga aggccacacc gggttcatga agatggcggt
      481 ccagggtttt cagagcccgc agaatgagac tgcgctcgcc gggcgtcatt tctccgggca
      541 gaaaggaaag ctgctgcatt gtccctgcct ccattcagtc gatgatgcgc ataatggcgc
      601 tgcattccgg atgctgcagg gcgtaatccc gcagccggta ataatggacc gtcatggcat
      661 aacactccgt acgacaggca tgatgactgt acgtcatcag acaggcagca atgccagcgg
      721 cttccgggct catttcagcg cggttgccgt tcatggcatt gaacagtacc catgtttcgt
      781 catcatcgtc atccggttcg ggggccataa atgccccgcc gttgttcaga gtgtacagat
      841 tccagatacc accgcagtag tcttcgcaca gacggtccat ccagccgaaa acacggggct
      901 ccagggtcac ccactgtgga atgaggccaa aatgctgcgg ccagaagctg atgcgctgtt
      961 catcagggac tatggtggca accagctgag gctggtcatt ccctgatgca gcggttacgg
     1021 aaacagaaga tgtggtggaa ttatgtgaaa tggttttcat gaaatgactc cttaaaggac
     1081 aaataaatgc gtggttttca gggtaatggc tgaatcagcg caggctctcc agcagggttt
     1141 ccgccatcac ccacaatgcg cggttgagct taatgtcggt gtcgatgctg tgaatggcac
     1201 gggtatggat acgttttcct ttagcgctgc gacctgaaat tccgcctttc agcatattct
     1261 cctgaatggt ctgataagca ctccacaggt ccttaccgta atcctcccgg cgtcgtggcg
     1321 tcagaatgtc ggcggtggtg acgggctgat gttcgtcacc ataacggtaa gtcagtgccg
     1381 cctgtgccag cgcctggcgt gccggtggcg gcagaatcag cgactgcatg gcatcacgct
     1441 tttcctcaat ccggtcaaaa acctccacca cctcgtaagc cccttcaatg actctgtcca
     1501 ccacatttcc ccggtgcgga acacgcactt cccccagaga ctgaccacag acgcatccgt
     1561 tctggcagac gaacctgaag taacccggca gcatctggta gctggaggta ccgtcatgag
     1621 agttgagcag aataatttca gggacatgtt ctccgtttat ctctccggcc cgccgcagac
     1681 gcagcatgtg ttttgtgtat ccccggcggc ccgggtcgcg cacacgggtc tggcaggcga
     1741 agaatggctg aaagccttcc cgctgcaggc tttccagtac ggtgatggtg gggatgtacg
     1801 tatagttctg gcttcgggac gtgtgtttat cttccccgaa aatccccggc acatagtgca
     1861 tcagttcttc gtgtgtcagc ggacggtcac ggcgtatctg gttcgcataa ccaaaacgac
     1921 tggctagtcg cataatttgc tccttatcgg tggttaagat ttactggtgt aataaatgaa
     1981 aaagccacgt ctcccggaga agacgcggcc tgatgggtga aatgaatgac gtttattgtc
     2041 tgagaagacc gtcactggcg agccgcgtat taagcacctt tccggcatcg ggttcagaat
     2101 tccggcagcg ccagcgtcgc tgaccttccg cgttgcggat aacccgacgg aaatgcattc
     2161 ccgggtcatc ctggataaac acattgcggt aagcggttat gacagcaacc gcctgcccgt
     2221 atgagaaaga tccttcagcc aggacatact ctgtgtaacc cggcatatcg gttttctcct
     2281 gataaataaa aagcctctgc cacgcgttgt gacagaggct gaagcatgaa actgacttca
     2341 gggatcagtt aacgtttttt ccggaaacgg taatcagcag tggatggtag tcctggggat
     2401 tgaaaaccga taacggcaga ctgacacgat ggccgttact ttcttcagtt gctttaatga
     2461 tttcggttgt ggcgacattt tccacgcact ccgtttccag aaatgcgtct gtggttcgcg
     2521 tggcattact gtcaccaaaa gcttccgttt ccatttttct ggtcaccagc gtctgaccat
     2581 atttgtcttt gagttgcaga gtgatggtga gggggccaaa tccttcatcg tttccgccat
     2641 tatccagccg gaactggtaa gcacaaatat ttcccgggag ccatatcgta tctgtattgc
     2701 gtatactgat gtaacgttga tcctgtgccc ggagtggggc agaccacgtt aaccccagaa
     2761 tgaaggcggt aatcatgcag gttttgaaca ggtgaatcat ggtatttacc tctctgagtc
     2821 atgacgatga cactgacaaa tcaggtgata aagcgtaaaa ggcgcagaat agccgttatg
     2881 ccggtaactc cgggggtaat gcttcttcca gtcggttaac catattgccg agatgggatg
     2941 catcatattc catgacgggg cgttgcctga tgatactgac caccagtggt ttgattaaca
     3001 tattggtcgc ggcccgttgt tgtataccgg cggcgaaaat gatctccaga gcattctgat
     3061 cgataacacg ggccagattc tcgttcagat tttcaatctc atgtccggtc agcaggaaac
     3121 tgccggcaag ccatgtcacc tgctcctgaa tcattgccag aattttgacg cgatcttctt
     3181 caaccagcgc tccggcaatt tttcctgata cagcggaggc aatcattccc cccagaacac
     3241 cgccaaccac acgccctgtc agtgcaccaa atggtcccag tggactgaac agtatcccgc
     3301 ccgccactga cccgacagct ccacctgcca caccggaaga tgccacggca agattcctga
     3361 tgaactgcgc accggagatc cgtccccgca acattttgat catgtcaggg ccggttgtga
     3421 cagctaccag tgccagagat gtcaccagcg tacccttcat tactttgttc agggcactga
     3481 tatttcctgc accggtcccc ttctgaagag ccagtcggac agtcgggctg gcagtcgaaa
     3541 aatcaatgtg cttcagcata ccctgaacaa cactgagccg gtggagttgc tgggttgtga
     3601 cgtagacagc cagtgtgcgg gtgaaggttt ttcctgcctg gacagcagct gtctgcaggg
     3661 cagcatcgcg atcgccggtg ctgagccaga agaccgatgc cgtcagggca aaactgatcc
     3721 ctccggccgc cagactgaca accgaaccct cggcaatatc ataagtgacc gattcgatgg
     3781 tcccgaaccg ggtgatatta cgggcctgag tataagtcag atgtccccga cgaatcagcc
     3841 gggacgcttc tgcgggatcg gttacaccgg gtactttacc ttcgcggatc ttattcctca
     3901 tggtttcaac agcgccggca tactgatcac gggggacttc cagttgcatg gggccattat
     3961 ttcccatgta acgatactgt ccgttttgtc cgtcgaatgc cgcaccgacg ctacgggctg
     4021 cagttgaaca atatttcgtc tggatttctg ttccgctgac cagcctgtct gctccatttc
     4081 tggcattatt atcaccgagg atccgtgcgt ttttcagacg gactctgtcg acaatatggt
     4141 ttccccgttc agcggcaaac ccatgtccct gccctccgga agcaaacatt ttctcatttg
     4201 tactccaggt ttccggcagt ttcatgagaa tcagaccatt cgccgttgca tcggaaataa
     4261 agctgttgta agggcgttca taaatttcct gacacagtgt ttctgtttcg cgacaaagag
     4321 aggagcagta ccattccgga tccggcttat gattttccgg ggcatgatgt acttctgtca
     4381 gcccgcagga ggcacagata cgaagctcat ccacgtgatg gaatgataca aaacggcgac
     4441 cttctgaaaa gggataccag tcaccgccgt ccaggcaacg atcgagaaaa tcgtcattcg
     4501 cttccggtga taaattcagg cggttccgga agagcgtttt cagttcatca ggtgtgaatg
     4561 acaggttctc tttcaactgt tcgttaaacc ctgccatgtt tctttcatac caggcattgt
     4621 tcagtaacca gtttccgtct gccagttcaa attccctcag ctctccccgg gaaatccgtt
     4681 gagaaatgag catcagccag atgcgctccg tcagtgagtc aggaaggctg taatcgtttt
     4741 gcaaactggt cagccacgat ttactgttga gcaccggttg ttcttccagt gtctggctga
     4801 tggcacaggt cagttttttc agcgcctgga tctggctggc cgcccatagc agcgtcacta
     4861 atgcagccag ccagtacaac aacccgcccc accacatcag tgacggtgaa atatcgtact
     4921 gtaacgcgat caatggtgcg catgcagaac acgccataag gaaaaaggca gtataaaaac
     4981 agcagtaata cccgttaatt tccttgctga tatcttctgg tgtgatcccg ttctgctgaa
     5041 tgtttttgat ctgcagggga acagccagca gaccaccggc gagagcggtg gtcagaataa
     5101 atttcacaga gtcaaaggtg tgagcctcca gcgcgatatg tttaccatca agtccccaca
     5161 tgaagctgat gatcattccg gcaatcgtca gaaatacgcc tgccagccag cgaatatgga
     5221 acaaccgggt tatgcgttcg gtgatatagc cagccagtaa ggccgggccg aacagacaca
     5281 gcaatatgag ccctgccagc gcagaagccg tgctgattaa tgctctgaac aaaaggcgaa
     5341 gaatggcaaa tcctgcgatc agcaggatca gcgcgccgac tatctgtaac atagtcactc
     5401 cgtgatgaat atcatgttat tgttaatgcc agcgaatgtg gcgttgaaga tccgttgcgc
     5461 cggtccgggt cccggtcatg atgaccggga ccacagagag gcgatggttc tgtcagaagg
     5521 tcacattcag tgtggcctga ccgttatacc cttcagcgct gctgccgctg acgctgtggg
     5581 cataaccggc ctgaacgccc agggtgatat tttcccggac acgggcttcc agtccggcct
     5641 gcaggtccag tgatgtgcca ttctgtgagg gagagaacgt catcccgctg cctgcagtgg
     5701 aagtccccac acgcatatct ccccgggagc tgaaggtgcg gataacagaa ggctgtaccc
     5761 accagttcac cggtaattca ctcacactgt gttttgcact gtcacgcagg ggggcacggg
     5821 atgaggtgcc ttcgctaaag gtcatatcgt tgtggctgcc cagacggaaa ccggcacgca
     5881 catgttgtgc actgccatgc ccgaacttca cataaccggc gttgtcctta ccgtcatcca
     5941 gggaaagtcc ctgccaggta tactgcagtt gtggctccag catcaggttg tcagtgatac
     6001 tgaagggcag accggtttcc agtgaaccca gccagcccca gccccgggcg cggaagtcgt
     6061 tattgtccgt tgacgctttc atgctgtggc gggttcccag ggccacaatg tcagcccaca
     6121 gtccggaggc gttgtgtatc agattcaggt atccgcccag gctgccggca tcatcccgga
     6181 ccgtgccggc acgggagccg tcatcatcct taacatcaac ggaagaatgg ccagcagcac
     6241 catatacccc cgcggtcaca gacataccgg caacctctgt tctcatcagg tcaccctcca
     6301 gacggacgaa tccatagctg ccgctgcttt ccggcgtggc cccacgggca ataccgccat
     6361 tgttatcgtg accgagatga ccgccctgaa tgctgagacg gacgctgttg ttttcaccat
     6421 ttacaccggt ctgatggctg cgggagcctg ccacaatccg gtcatagtcc attgcctgtg
     6481 tcagcatgga ggcatacagg gggacttctg cacgataagc attttcactg cgcagatacc
     6541 agctctcatc actgtcccgg ttgagggagt agttaaaggc accggcctgc agcctgttcc
     6601 cctggacaaa ggccccttcc tccgtggtgg caccgttaat ggcttcaacc acctgaatac
     6661 ccttaccgct ggtcgccagc cccgacgcac tgttgccggc gttcaccagg ttcaggatgg
     6721 tttttccggt tgccctgccg ccgtcaatga ccagtctgtc agcattgttc tgtgccatat
     6781 ccgggcgtac acgcaggctg atggtgccat tctgtccgtt caggtttttc actttcaggg
     6841 ttgccggtac gaacttccct gtgcgggtgg aggtgaaatg aatctgtccg gcatggctga
     6901 ggtcatccac caccgactgc accgtggcgt tatcggggat attccaggtg gcaccggagg
     6961 cgagagtgac attcgtgggg tcaatggcac cgttcagcac agtgctgccg gtcagcttca
     7021 gggctgtacc gcgctgagca atgacatccg tggtgacggt actgtcgttc agcgtcagcg
     7081 tgccttcatt caggttgacg gctttctggg tgagtgtggt gttgctgaca gtgagtgtgc
     7141 cacttcctga tttttccacg ctgccgttac cggtgagaga gcctccctgc aggagtgcat
     7201 cgccttcacg gatggtcagg gtatcgttgt tcaccgtctt cccggaaagg gtaagtatgg
     7261 cgccgttatt cagcgtggtg gtgcccgcca gtgtgccgcc cctggcggtg aacagtccgc
     7321 catttaccgt ggtatccgtg gccgtatcac cggcgttcag cgtgaatgaa ctgccttttt
     7381 ccagcatcag ggcatccgcc tgaccgcctc cgatactgaa tgcctttccg tcgctccggg
     7441 taccactgac agcggcaccg gaatcggcca gcagtacacc gccatttccc atggatacgg
     7501 tggtggcggt accaccgttg cggacatcca gcgttccgcc atcatccacg cgggtattag
     7561 tggctgtgtg tccggtcagc acatccaggc gtccgccatt ttccagtacg acattatcag
     7621 ctttaccctc cacaaccgag aatgctccaa ggcggtttat gccggtaacg gttgcagccg
     7681 tactggtaac cagtgcaccg cccgtgttct gggtgactgc agtggcttcc ccgccggcat
     7741 gtaccctcag ttcaccgttc tgatttatgg tggtgttacc gacagcacca cctgccttaa
     7801 caacctgcca gcccccctcg ttaattaccg tgttcagcgc aagtccgtcc ttgtgcacat
     7861 actggtaacc gccattcagt gtggtattca gggcccttcc gtgtaccgac tggtcaccac
     7921 ctacgtgaat gagagtgtca cgggctatcc cggaaaataa gataatctgc cgtccatttt
     7981 tgttgatggt ggtggattct gctgttccct ggaccttctg acccgtgtac gagttgtcag
     8041 aatccgggcc gccttctgcg ccggtgttga tgatggttcc tgttgccacc ccgccacttt
     8101 taacgctctg gtaaccgtcg cggttgataa ttgtaccggt ggcaaccccg ccctcatgaa
     8161 cccactgctc gcctctgtta ttcagcgtgg tgttcaccgc cagcccgttc aggctctgtc
     8221 ccccgccgtc acgaataacc gtatcactgg ctgttccccc ctccagcacg acctgacgac
     8281 catttgtggt aacagtggtg tttccggcta tcccgccatt ctgtatccat tgcccaccgg
     8341 tgttttcttc actgtccggc cccagttcca gcccggtact gatggtcatt ccgttggccg
     8401 taccgaggac aatctggttg tcatgatttt ccagtgttcc gccgttcacg gtttcacctg
     8461 cctgtacaat actgtcagca gccagggccg ggactgacgt gacagcagca agagacagcg
     8521 caaccgccac accggcgcct tttccccgtg agcgggccag ttcggaggcc accaccaggg
     8581 tgcccgtaat gtgattccat accagcctgt agctggtgtt cagatgtcgt ttcatcagct
     8641 tttccttaca gagggtgaat aaaaaagccg gtacccacaa atgtggatac cggcaggtac
     8701 aaaacagcat ggtcagataa agtggttttt cccggacata aatgcttccc ctctctcatc
     8761 cgccagagaa gtatcagtcc gttgtgtgca attccgttct gcatgatgaa gagattcagc
     8821 tgccttttcg caggccatta accactataa acgatcgata tcagcgatcg atacctgttt
     8881 tatcgatcgt tttattctat tgatagcagg gcggtcacgc aagagaaaca ggagggtatg
     8941 agggggttgt gtcatgtttt cagttggtta acgtaatgta atcaggtatt tccctggcag
     9001 actgttcaga agaaaatcca gttccataca gcgcgggcaa cagagaccac cgagtcacgg
     9061 acggcacgca gaaccgtgcg tgcaacagag gcaatacaga cgctctccgc tgtgtcaaat
     9121 atccggtcca ccgcaccggt aaactgttca cgggcctgag accggacaga ttccgtgcgc
     9181 agctcgtcct gcagtcgggt catcagggga ctggcggcat ggtcgggaag cgctgtcatg
     9241 agcgcactga ccagcgtatc cagttcccag ccggtgcggg ccgatacggc cacaaccgga
     9301 tgtacgggcc ggaacagacg gaataccgcc tccgtttttt cgcgaatatt ctgtgcctgt
     9361 gcaggagaag gctgaatacc ggccatatcc cattcatggc agggctccgt tttgtcggcc
     9421 tgcgtcacca caaacagtac ccgctgatgt ccccggtgca ggatgtgtcg ccagaaatac
     9481 tcatccacag acagggcacg gtcatcggct ttaatcagcc acagtaccag gtcaagttca
     9541 ggcagaatgt cacggtacag ggcctcatac tctgcatccc tgtcccggct ctcgcccacc
     9601 ccgggcaggt cagtgataac catgctgtga ccgtggccac tcagacggaa gcgccgtact
     9661 tcccgggtgc cggcgtgaac atcactgacc ggggtgacct ccccctgaaa cagtgcatta
     9721 cagagtgagg atttaccggc cccgctttta cccataatgc caatcacggg ttcgtgacgg
     9781 gtgagtttgc gcagatgttc caggatgtga cgggaaagcg agtaaggcag ggaggagagc
     9841 ggtttttcaa ttgcctcaat ggcatcagac ggattcatac atgtattccc ggtgaaaaaa
     9901 gataaaaccc cgcgcaccgg aaaggtggcg ggggattcac ggagataata tctgtctgaa
     9961 attgttttga atcgatggga ttgttgtgta ttaacatgaa ggagatcagg acttatccgt
    10021 tactgggtga tctccttcca tgaaaatacg ccgttattgt taaacagcag gctgatttct
    10081 ccgtaaaggg gaaaatattt atcggctgca tgattatcag gtgtgtctgt gtttactgct
    10141 gtgagtattt ttcagtgtat tgtgggggag atgtcagtgg gagatattaa atttacgttt
    10201 ataattatct tacttcttct gtgtgttttt tataagagtg cacacgtaat gatatctgtt
    10261 gataccatgt ggttaagtgt ttcccttcct ggtcatgcgt aatgattaaa tgcttttgtg
    10321 acaccattct tccggtctga atatttatcc cgttcttttt tatttgtgca gtgatactgt
    10381 cggaagagaa ttcagtaatg tgaggtgtcc tggtcaggat aatatctgct gagatattat
    10441 tagttgagtg tgtttttact gagcaaaagc tggcttttaa ttcctgtact gaaacgctgt
    10501 cgctcgtccg gggttaactg aaactggatg atgatgtaaa atgaaggctg tttttgtgtt
    10561 tgtcgaagaa gtgttatttt aatccattct catcattggc tggcgattat acagaagtat
    10621 ttgaagaaaa aatggtgcat tttatctgtg tcagcaggga agaagtattt tattgatgta
    10681 tcagtattaa atggtttttg ttatttataa aaatgtcatc ctgtccataa aatattgtac
    10741 tcagcattca gtgcaggaac tgtagtcttc tttattttat tcttcttcag gaggggggtg
    10801 attcctgaag gcgttattcc cggtgttctg tttatgattc ttcccggcag gaaaccggta
    10861 tctgcaatac ccgggatgat gctttttgtt cgtacgtggt tgtgtaaaat ttgaatagtg
    10921 gatgtgctct gaagggaagg aggggacaca cagattcatt ttgtctttct tcagcagcaa
    10981 aacacactgt ggttattatt taaccattaa atcaacaaaa atttttacct catattgatt
    11041 tgactgctat ttttgctatt aatagccaga attgcagtgc ttttatatct gtgtgtagtg
    11101 aggttactat gtcattcggt cagccttgtg atgagtttcc cctgagttcg ctgcctccac
    11161 tcattcgtga tgccgttatt gaagctcagc aaattacgca ggctcccctt gggcttgtgg
    11221 cagcgtctgc cctgggcgct gtttcacttg tctgccagaa tctgattgat gtctgcagac
    11281 tgaatacact gcgggggccg gtctctcttt ttttcctgac gcttgccgag tccggagaac
    11341 gaaaaaccgc agtggacaaa ttattaatga agcccctgta ccagcaggag atgcagttat
    11401 attccagata caaaagtgaa ctggctgtct ggaaaaacaa ggaagagctt ttaaaggctc
    11461 agaaaaaagc attgttgtca aaactgaata aagaactccg caaaggcgcg gatgagtcag
    11521 aaaccctcag acaactggaa gtacttcaga aaaacagtgc ggaagaacct gtgaggtata
    11581 aattcatttt taatgatgcc acgactgcgg caattaagaa tcagctttgt ggtaaatggc
    11641 gttcagtggg tattatgtca gatgaagccg gtattatatt tgatggatat accctgtctg
    11701 agttgccttt tattaacaaa atgtgggatg gctctgtatt gtctgtggac agaaagaatg
    11761 aacccgaaca gatgattgaa aatgccagaa tgacgttatc cctcatggtg caacccggtc
    11821 tttttgaccg atatatggag cgaaaaggtt ctgttgccag ggacagtggt tttcttgccc
    11881 gttgtcttat cagtaaacct gcaaccacgc aggggaaacg ctttattaac ggagctgtga
    11941 ttccgggagg atcattaacg gcatttcatg aacgattaat ggaactggcc agaggcagta
    12001 ttgaaaaatc cagtgaggat gaacggtatt gtctgcattt ttcgcctgaa gcgcaaaaaa
    12061 tatttattga gcactataat gttctggagc aggatttatc cccttccgga cccttaagcc
    12121 cgtttcgggg acatgtttca aaaaaaacag aaaacattgc aagaattgca gcgttatttc
    12181 agtatttcag ctatggtgaa gggaaaatct ctgccgatat catgacgtct gctgtcgtaa
    12241 taagttcgtg gtacacagat gaatataaaa aattatttgc gttacctgat gaatctgaac
    12301 tgcagcagaa ggacgccgag gagctgttcg actggctcat tgaggagtgc cgcggagaat
    12361 gcccgccccg tgtcaggaaa aattatattc tccagtgtgg cccgggccgg ttcagaaaca
    12421 ggaaaaaact gaacgcattg ctgaacattc ttgaatctca gttcaggctt tctgtcgtgc
    12481 cggaaggaaa aaccatgtat gttctgcttc cccagatagc cagtttaaag ttgtctgatg
    12541 tctctggtat ttttacctcg ggttatcact acaataagct gagagcgaag taaccggtga
    12601 gcagtttttc tccattatgc ttcagactta gatgattgat tattatgggg atttcatcac
    12661 cgatattcag atgtatgaat atcggtgatg aactaaagct caggagctgc gtggtggtga
    12721 tgaatcaaca ggcatataac tgatacccgc cggtcagaag ttccagcctg cgactttcct
    12781 gtctgcgtgc ccgcagtccg gcgttcaggt gcagcgtgtg acagaacgca cggagagcga
    12841 aacggcaggc agacaggccg ttcagtcagc cctggcaccc tgcccggata ccagcttccc
    12901 tgaaacaact ggcatcaggt gcgcttatcg tcaaccgttg tgcggtatgc caggagcacg
    12961 tactgatcca tcagatccgt cagtgctgcg ctctgcttct gacggttgat gatccattcc
    13021 tccagctctt tttctgattt ccgtttcaga ttctccgtcc agtcgtcaag gcagtttccg
    13081 gtcattaaat caagccagca aagccagacc agtttgaggc gcaactccgt gccaccaaac
    13141 tgacggaaga ggctgatcag tttatttgcc agcgaacgct gcctcagacg ttcacattca
    13201 gacaggagaa tgccttccag catcggatga tgaaccagca gatgttcact ggtgcataaa
    13261 ttcactatca gatcatcatc caggggcggc gtggtccgtt tgtccggacc ataaatcagc
    13321 cggtgcagac tctggggtac gatggcaatc tccggttttt caccattgaa tgacacccac
    13381 ccgggcgtca gattatggaa gtagtagata ttcggatgac acacgcccca ggatacggcg
    13441 tgccgggtga agccggtcag ccatttctgg tagctttcgt ggagcagctc gtaacgggtc
    13501 tgatactgtg gtttcatttt tatattcctc acgggggata acaggcagca gaacgccgcc
    13561 ggcaggccgg agactgcgtc tgctgcagat gaacggttaa gaattacgga cgggactgtg
    13621 caatacgcgc ctgcagccag gcttccactt cgcttttcag ccatcgggag ctgcggccca
    13681 ttttgatggg cgcaggaaag tcaccatcct tgatgagttt gtaaaaccac ttatcggtca
    13741 ggccggtcag ttgagtgata aatgccatgt tgtagtggca cactaaattt ggccacctga
    13801 tgaaaggtga tattctcacc acaacacaaa acaggtgact taatgaacaa gaaaaccaaa
    13861 cgaaccttca cccctgagtt caggctggaa tgtgcacagc tgattgttga taagggctac
    13921 tcatatcgac aggccagtga agcgatgaat gtcggttcaa ccacgcttga gagctgggta
    13981 cgccagctca ggcgagagcg ccagggtatt acgccctctg ccacacccat tactccagac
    14041 cagcaacgta tccgcgagct ggaaaagcaa gttcgccgtc tggaggaaca aaatacgata
    14101 ttaaaaaagg ctaccgcgct cttaatgtcc gactcgctga acggttcacg atagccgcca
    14161 gactaagtga cagccacacg gttgtcagcc tgtgttctgc tctggaaata caccgcagca
    14221 gttaccggta ctggcgaaaa cgacgcgata cggttaatcc ggcacgagtc aggttgtgca
    14281 gcgaaatacg ccgggcgtgg aaccaaagtc ggggctctgc gggcgcgcgc actctggctg
    14341 aaatgctgac tcaaaacggc gtcccgatga gccgttaccg tgccggacgt ctgatgaaat
    14401 atctgaacct gagcagttgt cagcccggaa aacatcagta taaaaatgcc cgtcaggagc
    14461 ataccagcct gccgaatctg cttgagcgtc agttcgcagt accggagcca gaccgggtat
    14521 ggtgtggaga tattacgtat ctctgggcag gaaatcgctg gtgctatctg gcggttgtta
    14581 tggatctttt tgcccgcagg gttatcggct ggagtctgtc agcgcatgcc gataccgcac
    14641 tgataagcag tgccctgcgg atggcctatg agacgcgtgg ccaaccccgg gatgtcgtgt
    14701 tccatagcga ccagggaagc cagtatacag gccttaaata tcaacaactt ctctggcgtt
    14761 gcagaataaa tcaaagcgtc agtcggcggg gaaactgctg ggataacagc cccatggaac
    14821 gcttcttccg cagtctgaaa acagaatggg tgccaacgaa tggttacgca ggcaaggacg
    14881 aggcccggca gcaaattaat gattacatat tgaactacta caacagcgtc agacctcacc
    14941 attataacgg tgggctgacg ccggaagagt cagagaacag ataccatttt tactgtaaaa
    15001 ccgtggccaa tattacttga ccactacagt tatccgcttt gtgattctta ttggctcttc
    15061 tcaggttatt aaaagctgaa tatgttcctg ttgtcgggaa ccaggataat accgggaacg
    15121 ttaagcggta tagcgacaac aaagaattcg aaatcaaaag ggaatggtgc cggaggcgtg
    15181 ggcggtaatg ggctgatttt caggtattac cggcttacag cgtgtggttg atattgaggg
    15241 gctgtgagtt tcacgtattt gtcagaaacc cagacgagtt ttcctgtatg ggacgaaata
    15301 aggcagcggc aatacatgga aaaatttaat atggtatgaa ggagaacctg tcggttacgc
    15361 ttaatataac cggtacactg tcagctcagg ggatacatga accgttatct caccagctac
    15421 acagtatata cacactgacg ctgacagtag tcagcaggac cacaataatc agtgccagca
    15481 gtatggtaac gatacggtct gtattccggt aatagccgaa ggggtcgaca gaaccacaat
    15541 acgggcaata cacagtatta tgagtaatcg cgtttccaca gtccctgcac ttctttttgc
    15601 gtatcaccat aacgccctcc tcacagcctt acccgtaacc ggttttttac attaaaaaaa
    15661 caacgctgaa aattcaaccg tcagtccgga gacgaccgtt cgggttatca cagagtgcct
    15721 gagacagtgc cctgccggag gtcacttcag ggactctgcg cattttttac gacgtggcta
    15781 ttccgtatga accatacgga gatttaacca tgacctacaa atacaacccc ttctggcagc
    15841 aacgtattcg tgagacagtg cggcacgcac tgaatgttca tccccgcctg acggcattgc
    15901 gggttgacct gcgtttcccg gatgtaccgg cagcaacgga cgcagctgtg atatcccgct
    15961 tcatcaatgc cctgaaagcc cgaatcgacg cttaccagaa acgtaagcat cgggaaggta
    16021 aacgcgtgca tcccaccact ctgcattacg tctgggcccg ggagtttggg gagtgcaaag
    16081 gtaaaaaaca ctatcatctg atgctgctgg tcaaccggga tacctggtgt cgtgccggcg
    16141 attaccgcga tccggggtca ctggccggaa tgattaaaca ggcctggtgc agtgccctgg
    16201 gggtggatgc cggatgttat gccacgctgg tgcattttcc ggtcaggccg gcggtgtggc
    16261 tggaacgcga tgatgaaacc ggctttcagc aggtgctgga acgtgctggc tatctggcga
    16321 aggagcatac caaagctcgc ggcaccggtg agcgcaactt tggctgcagt cgtggctgag
    16381 cccgacagac gacttatctg accttagtgt gatgacgcgt tcacagcgac cacactgatt
    16441 ttcccttctg agactgaact gaaaaccgct attcacacct gaccacgctg cccgtaccgg
    16501 gacggctgtt gcctgcgtgt cgtctggcgg ctgaggaata tgactatgta cgcaaaatcc
    16561 tttatcgctc ttgatggcaa cggacgtctg acgggcgccc gtacagcaca ggccgcacct
    16621 tatgctaact acacctgcca cttgtgtggc agtgcactca gataccatcc gcaatacgac
    16681 actgaacttc cctggtttga acacactgac gacaggctga cagagcacgg tcaacagtgc
    16741 ccttatgtca ggccggagcg cagagaaata cagttgatta aacgtctgca gcaattcgta
    16801 ccggatgcct tacccgtggt gcgtaaagcc agctggcact gcagacaatg tcaccacgat
    16861 tattatgggg agcggtactg cacacactgc cagaccggag gattcagtct tccccggaca
    16921 gctcagcaac tgtcttgcac gtaaaaatta tggagcaacg tgatggtacg attcattcca
    16981 ttcttcgttc tttctgagca ttgcgttcag gatagtcagc agattgcgta tacaggtaac
    17041 cagcgccact tttttgggtt ttcctgcggc cagcagtcgc atgtaaaaca cctttattac
    17101 cgggttaaag cgggttgcga caagcgctgc catgtacagg gctgttcgca caccagccct
    17161 gccgccaaag atggttcgtc gccctctcat cgtgcccgag tcccggttta ctggaaccat
    17221 accgacaagc gcactgattt cacgcctgga gaggttaccc tgttcgggta cttccgccag
    17281 cagcgcagca gccgtcatgg tgccaacacc tttaatactg ctcagtcgct tagcaagctc
    17341 tttgaagtgg ctatggatat ggctgttcat gtcagcatct agtcgcgcaa gctcgtcttc
    17401 cagtgctttg atgatgatat taatgctttt cctgttctat ggatgagcag ggtgcaaccg
    17461 gtttctctcg gcaaccagca tcgtaatcaa ctgccgccta cgaactacca ttgcagtgag
    17521 aacctgacat tcagtatccg gcagtgctct gataaagcgt tcccgctccg gatgccggtt
    17581 gagaacctca gccatctggg aaagcactct ggcatcaata ctatcagttt tcgcgaggta
    17641 tcccatggcg cgggcaaagt cacgagcttg tctggggtta accacggcaa gctcaaaacc
    17701 ttctgcctga agcgcacaga ctatggcggt ttcaagtcct ccagtggctt tcattaaaac
    17761 cagcgcaatg gcgtacattc tcaactcagc aatgatggcg tcaaagccat cactgtcatt
    17821 gccgacagaa aattgttcaa tagcgctgct ggcggcaata tccagtatcg ctttcgaaac
    17881 atcgataccc acacaaagtg aacctggctg actcatgatt acccatcctt gcaaatgcgt
    17941 tatggcgtac tgacaactgt tcgggtttct gatgagtggc tcagtgtatg cgccattcgc
    18001 tgagctacgg gctatgaacc ccttgaggga tcgggctgca tacaccttgc caaatctaat
    18061 cacagttatt ttaaccaatt gtcaagatac aaggaggaga tatgcgaatt ctgaactgct
    18121 atatggcgaa tgacagcaaa ggccattttg ttacggcgaa agaagccgcg aagaacaacc
    18181 gacaggacgt tttgtgctgt gtgtcctgtg gttgtccgtt aacacttaag cggggcaatg
    18241 acggacaacc gccgtggttt gaacatgacc agatgactgt tgctgcgaaa atcctgctgc
    18301 gatgcacctg gcttgacccg gcagagaaag aggcccgtcg tttgcatctg cagggtatga
    18361 cggttccgga ttatacggtg aaggtgagaa agtggttttg tgtgatgtgt gacgaagatt
    18421 atgaggggga aaagtgctgc ccgcgttgtg gtaccggggt atacagcagg gagagggggg
    18481 ctgcaggagg gcaacaggaa ggataggaac tgagcggttc agtcgcggaa atcagttgca
    18541 gagctgtaat gatatctcct ggagtgctgc gttggtaggg gcatcccggg atagagaaat
    18601 tttagcactt tgccctgaat gattgtagac gtcccgagta ctcgtaccgg cctgagttat
    18661 tacagttaga tattcgcttt ttctcccgca tacttcacag ctgtcaacga cggatgaaaa
    18721 gtggttcttc cgcaacttgg gtggaagaaa atggatgaaa ctttctggtg aacaaggatc
    18781 taagctgatt taagttatgg gcaacgcaat gcactctctt aagacacttc taaagttacc
    18841 ttgcggatgg cgatgcagtc gacaaattat tagctctgac ggtataaccc tccatctcca
    18901 cggaaaacgc aaaacagcac aatgtcctga atgctttaag cgtagcgact ctgttcatag
    18961 ttgtcgtcgg cgccggatac agcatctacc ctgctcaggg cagacgctat ggcttgtatt
    19021 ttccgtccgc cactggtact gccgtaaccc ggcttgtcac gtaaaatttt tgccgagtcg
    19081 cttgctccct tcgccagttc acaccagcag tcttcacagg cgttacaaaa ttaacaacgt
    19141 caactgggat taatagctgg aggtgaggct ggaaaacggg ctgcaacggc agcgggtctc
    19201 cgttgcagtg ctgatactct tcttcgcagg cttatcaata ccccggagac gaaacagtca
    19261 ggcgcgcctc atgtcggtat tgatgagtgg gcgtggcatt ggggccaccg ttacggtacg
    19321 ttaatattca atcttgatac tcaccgcccc ctggtcctgc ttcccggccg tgatcagcgt
    19381 acgctggcga cctggttcag aaaaggtccg gaaatacagg ttgtatcgcg tgatcgcagt
    19441 ggagtctatg cgacagcagc acgtgaaggt gcacctcagg ccagacaggt ggcctatcga
    19501 tggcacctgc taaaaaatat tggtgatgag cctgaacgaa tgatgtacag acatatgcct
    19561 ctgatacgtc ttgttgtcag agagttatca ctgaagaaat cacctgagcc agaaatatct
    19621 gtgcctgtag catcgctccg tcgtctggaa cgccttaaac agcacacccg caaaaaacgg
    19681 catcagcgtt ggacagaggt tatggccctg cataacaagg gatgtagttt cagggaaata
    19741 tcccgtatta caggcctgtc gcgagtgaca gtcagtcgct gggtgggttc aggaacattc
    19801 cctgaaatgt caactaggcc tccaaagcga gggcttctgg acccatggag ggagtggtta
    19861 aaagagcaac gagaaagtgg taattataac gccagccgga tatggcggga aatggtggcc
    19921 cggggattta caggcagtga aaccatcgtc agggatgcgg ttgccaaatg gcgtaaaggc
    19981 tggaacccac cggttactac tgccgtcaga cttccttcag tgtcccgggt aagccgctgg
    20041 ttgatgccct ggagaataat caggggtgaa gaaaattatg cttcccgatt tattagtctg
    20101 atgtgtgaaa aagaaccgga gctgaaaata gcgcagcaac tggtactcga gttctaccgt
    20161 attctgaaaa cccaaaataa atcacagcta agcagttggt tcactcgagt ccacgaaagc
    20221 ggttcagcag aatttcggcg cgtggcggcg gggatggaag ccgatgctgc ggctatatgt
    20281 gaggcaatca gcagtcgctg gagtaatggt gttgtcgaag gtcatgtaaa tcgcctgaag
    20341 atgctgaaac gccagatgta tggtcgagtc ggatttgaac tacttaggcg gagggtaatg
    20401 agtccactgg catgaaaatc ttcacctagg ttgcggaaga gccacttttg cactgttcgt
    20461 gacagtatgg cctttatgta ctcactttgt taccggtagc tggatcatca ctaccgtgct
    20521 gcaaatggtg gtgaacagca tcaattgtgg aatgaatgtt gccgaagcga ccaatgcgcc
    20581 tcgtttccac catcagtggt tgccggacga gctgcgtgtt gaaaaggggt tagtccggat
    20641 acgctcaagc tgctggaagc aaaaggtcag aaagtggcgc tgaatgaggt gttgggcagc
    20701 acgcaaagca ttatggttgg gtcggatggt gagttgtacg gcgcatccga ctcgcgcttg
    20761 gtggatgatt taacggcggg gtattaattg acatcccccg gcgcaatgtg ccgggggata
    20821 atggttttat gcggtcaata atcgtccggg attatcgata cagagttttt tgaggatcgt
    20881 tttatcgacg ccgcgctccg ccagataggc cagaaaaaca tctggaacaa atccccagcc
    20941 attaccgcca tttttagccc acatttgttt aaggaagaca tcgtggctga gaacaagctg
    21001 atcggcgtat cccaactcaa tgagatgagc gacggcatcg gcagtctcct gcacccctgg
    21061 cgctattccc tctttcggga aggtaatgtc taggccaatc atgtcgaatt ccagccagac
    21121 acctctatca agcattttcc gctgatacgc cacgtctttt cctgacggat ccgagtgtgc
    21181 gagagagact ttatttggcg agacgcccat ttcacctaac acaatgtcga gtacttcatc
    21241 accgcgacga agcccgcaag ctgttatgct cggcttcggt aaatgtcggt gaaacaccta
    21301 tttcaccgat cattccggca cgaatatccg tatcgccaat cccttgattc aattctttat
    21361 cgatcgttgt cgccagttca tcaaccgttt tatgaattct ctggctttca aatttttcca
    21421 ggtagggacc ggaggaagca acaatattca gacctgtttt taatgcgact tctcgtaatg
    21481 cctgcgcatc tcgtccgata gactcagacc ctgtggcatc aacaatggtg cgaccaccga
    21541 gcgagataaa gttattaatt tcgaagatca catcttcaat aggtttgcgg tccatattat
    21601 cagcgcagca ataaggatcg tgttttaatg cccactggat ttctgccgtg acttttttat
    21661 caacaagccg ttgtgaaaaa ggatagcaag gagcatccac aacagaggaa agatcgttga
    21721 acagatgttc atgaggaagt gttagcccca tatcttcacg ggcgacgggg cccgtcacag
    21781 tttgtagata atctttcatg attatacctc cattttcttc attgttttcg aggttctgta
    21841 ttccggaata gtggtaacaa ttgcaccgac cagtgcaaac agtgttccga tgatcgtgac
    21901 cagataaacg ctattgccca gcgatggaat taatacgtca atcaatactg atcccagtaa
    21961 ctggcccgcc gttgaagcaa cccccagcat cagtaacccc agacctctga ccaaaatggc
    22021 cattaaaccg atagacatta gtccaagcgg gccgccaaga tacatccacc agacatctgg
    22081 taattgaaca gtgacgtgcc ctaatgcgac acgaatagct agcgctgtgc ctaaaacaca
    22141 aaatccaacg atgaaattct aggtgattga aaccagcatg gatcccgtag cttcagctac
    22201 ttttgcattt ccagcgggct gccatcctgc aagcaaacca gctaagaacg gaagaatggc
    22261 aagtaagatg aaagaggtag aatgccactg aggtgacaca acaaaaatcg tagcaataac
    22321 cgcaaataat gcaccgatga tgcgataagg ggtaaagtat tttttctcgt cgacaccgat
    22381 accaaagcga tcgcaaagta aaccagacag caatagcgcg gagatcagtg cggtttgaaa
    22441 cgttgcgacc ccgagtgcgc tggctgatgc gccttcagaa aataccacca tcgctccaca
    22501 gagaccggca aaccagttcc agagaggaac ttttcggttt ttaatcagag aaggaattgc
    22561 cgcaaattgt tgccgggttt cttttcgcgc cataataata aaaaacatca cgactaaacc
    22621 actggcaaag gagataactg cgcatgcatt accatcctgt aaccagtgac ctaattgacc
    22681 attcaccgct gattgcatag gggagagcat acccgcaaga atcgtggcaa gcatccataa
    22741 gggagttgag tacttattct tttttataca tcaatccttc tattatagta aatggagtaa
    22801 ctggagttac cccattatta ttattatctt gcaatatgag tcagtatatt tacccacaac
    22861 gcggtataga acggccagga cataaactgc tgtgtacccc agtgcggtga acagtcactc
    22921 ataaagcagg ctattatccc gttgtgatag ttaccaaaga ccagtaatgg agcgttgttt
    22981 attgttaata ccacctcagc attttcttta gcgatcgccc ggttataacc taggaaaaaa
    23041 ggatattcag agaacccttt aataactgga tgttcaggtt gtgatggctg tgcaataaca
    23101 ccctctggtt tttcgacacg atcgtcacca tctaacatcg taaccgggag cacatcagcc
    23161 agaactgtat ttttataatt ggcttttgct tcaattccca tgaatgagag atagccgcca
    23221 atcatcaata acccaccacc gttattcaca tattctttaa tcagttcgag agcgtttggt
    23281 tttatgcgaa gctgataaaa agtatcattt tgtagcagga aagtattact accaatatca
    23341 ctaataacga tggcatcata ttgttctaac tgtgccacat cttcagggaa ggcaatttgt
    23401 accgtatgtg ctggcatata ggtgacatct acctgactat ttttcagaca ctggagtaac
    23461 caggtcgcac cttcttcata tttacttgag gtaaaactgt catatccttt tgaatgaatc
    23521 atatgaatat gccaggattc cccaataaag agcactttta gtgatttttt ggtgttattc
    23581 attctctgtc ccttcttaat gcgtaaaaat agtttgctga taatgtatac tctgaattcg
    23641 atgtatcaca gattcatgtt caggcatgtc ggaagcattg cttgtctcca ccgctaggga
    23701 tgcaaaggcg ctggcataac acagtgctga ttccagtgat tttcctttcg caagtcctga
    23761 tgccagagag ccgttaaacg catccccggc tcctgccgta tttttaacga ccgctggaaa
    23821 cgctggcgaa tgaatttttt gccatcaaat gcgagcgagc ccttgctacc gagcgtgata
    23881 accgtgtttt ttaccccttt attgtggata gcttctgcag ctcgtttagc tgattcaaga
    23941 tcgtgtacgt ctatgccgga cagtaaacca gcttctgttt cattgggggt gatataatca
    24001 acatcctgaa taagttcgtt gacgatatca ttatagggtg ccgggttaat aatgaccggg
    24061 atactgtttt tttgcgccaa tgtgatcgct tgttgcagag cggtataatt tgtttcgagc
    24121 tgcagaagta tgacatcaga attaataatc gcttctttct gaatagttat ttcatcggac
    24181 gacattgtca tgttggcacc gggatagatc gcaataacat tatcgccagt accttcatta
    24241 acaaggatcg tggcagttcc ggtttgcgtt tctttcgttt gataaattat acttttataa
    24301 atcttagacg aattcataaa gttgatcgca taatcactaa agtgatctgt acctactttt
    24361 gttatgaaat aaacatcagt atcggcaaag cttgctgcca gcgcctggtt acagcctttt
    24421 ccccccggtg aaaatataaa atcacttgcc agaagcgatt ctccgatagt gggaagcctc
    24481 ggcaaataac tgatgatatc aacattaaac gaaccaataa cgcatacttg acttttcata
    24541 gttgagtggc ctttttttat tccctgggct gtatcataat tttcaagcgc ataagcgatt
    24601 tcatttttcg caatagtatc aagcgagtct aggacgataa aagcaccgcc atgacatcga
    24661 gtcagatagc caagcctggc aagttcatta agatctgaac gaatagtttc tttcgttaca
    24721 ttgaggctat tggacagatc ggatactttt acagaaccga atttactgag tcgtttcaat
    24781 atttcatgat ggcgttcaaa tttcatgctt cacctcactt cagttacgtt aatatctatc
    24841 gatgtatatt gatgtgataa tcaccataaa gtggcgggga aaagcaaaaa aaagcaaaaa
    24901 tagcctgaga aaaggtaagc gttaatggag aatcatattg atgcttattt attggtgaga
    24961 actacgttcc atggcaggag ttcatcaata cggttggaag gttattccgg cagtacgctc
    25021 aggatatggc gcagatacgc ttccggatca atgtcgttca gaaggcaagt gccgataagc
    25081 ccatacaaca gtgcactgcg ctcgccgccg aagaacataa agtttttatt tccgagacag
    25141 atggcacaaa gggctttttc cgcggcatta tgtctgcctc cgcctgaccg tcaacactgt
    25201 aataacagag tgcgccccac tgattcagta cacagctgaa ccattctccc agtctggatt
    25261 tttggatagc gtggcatttt tctccaccat gcatgcatgc agtgagtcca gtaacagttt
    25321 gctcttcttc tgcctgacca caagatgtgc tggactggta aacgatgggg atacctgctt
    25381 acgcgagagc aataagtggc tggccacaat tctcggtaca aaactctgct gaatagcagc
    25441 gattttccca gcgtggtgcg tcgtttgcat tttactggtt ctaagttaac aattatctgt
    25501 tcactaatga ctactctaaa aacttgtatc aggactatca cttatttaag tgatattggt
    25561 tgtctggaga ttcagggggc cagtctataa actttttaca aaaggatgaa tgcagaaggt
    25621 tactctaatg gccagagtga tgacaatgat ggtcagacgt tgacctgcag aaactgcatt
    25681 ttaattggtc tataaagtct gcaagaggaa tcagggtcag atcagtgagt cttatcgcgg
    25741 cccgaagcta tgactgaaag aaggctggat acagaattgc aacaatttgg caatgtcaga
    25801 acacagaagc tttgcaagca ggtgaaaggt tatgtctagc aaaaggagta tatcagaata
    25861 catctactga tgtacaaaac acaacaatag cttcatgata tataaatacg tgatgacgag
    25921 tggtactccg catggttatt taacttctca gcaccactaa tgatcctgag caacttcgta
    25981 tttgggccat tgccatggag tagaaagctg tgtctgagaa tgcagaattt gagaaaagaa
    26041 ttcgctttct tggagagctt ctgaaaatgg cacgctaaca acgttttgtt aaaaaatgtg
    26101 aatcacttgc cggggctgca ttttctattc gaggagaatg ttgacgtaga tattacagaa
    26161 atctcagcgc atcaggataa actgctacct taggtcaacc accaaaagaa aatacaatac
    26221 gtccagtttg tatgccttta ataacacatc ttttaaatgt tgaagaatta tcccgcctgc
    26281 tgaagaacgt tgctctgagt gtgataaacc tttttattct tgatgatgtc agcgagaaga
    26341 tggaatatat ccccgactgg gtcgtggtta cccggttaaa catctggtgg cgctaataaa
    26401 tctggcaagt cacattctgt tgaatgctgt gacagtgcct taccgagaga gcgaaattgt
    26461 ggtgttctag tgcatagtcg ccaccattcc ggataattct attgcgttgt tttacaagtt
    26521 cttttacagc gcagatctgt cgctgacgtt gaatcaatag cctggatttc agaacacaat
    26581 gcccgtttgc tggttggtcc ggcagtagca ttaattcatt atctgaatga aatgtgtcaa
    26641 tccacagaaa ccgctgtcaa tgacacattt tgtcggacgt taacacgaga aagtctggct
    26701 attccgtaat gccatcttat ttcgtaatgg attgagaatg ctggattttt aattatatta
    26761 cgcgcagtat taatcgcgta aatgttttgc ggtcagcaac ggtgggggat ccattgttct
    26821 gttcaatgaa taccatcata catttgaatc aggagtcggt atgatgattt tggtgacggg
    26881 gggcgcacgg agcgggaaga gtcgccacgc agaggcgctt attggggact cttcacaggt
    26941 tctgtatatc gccacctcgc aaatccttga tgatgagatg gctgcacgga tagaacatca
    27001 tcggcaaggc cgcccggagc actggcgcac agtggagcgc tggcaacatc ttgatgaatt
    27061 aattcatgca gacattaacc cgaatgaggt tgtgttgctg gaatgcgtta ccacaatggt
    27121 gactaatctg ttgtttgatt atggcggcga taaagaccct gatgaatggg attatcaggc
    27181 gatggaacag gcgattaatg ctgagattca gtcgttgatt gctgcctgcc aacgttgccc
    27241 cgcaaaggtt gtattagtga ctaacgaagt gggaatgggg attgtgccgg agagtcgtct
    27301 ggcacgacat tttcgtgata ttgccgggcg ggtaaatcag cagttggccg ctgcggcaaa
    27361 tgaagtatgg ctggtggttt cgggtattgg agtaaaaatc aaatgagtaa attattttgg
    27421 gcgatgctct catttattac gcgtctgcct gtaccgcgtc gctggtccca gggactggat
    27481 ttcgagcatt attctcgcgg tattattact tttcctttga ttggattatt gcttggcgcg
    27541 attagcgggc tggtcttcat ggtgctgcag gcatggtgtg gcgcaccact ggcggcactg
    27601 tttagcgtac ttgtgctggt gctgatgacc gggggattcc atctggacgg cctcgctgat
    27661 acctgtgatg gcgtattttc tgcacgtagc cgcgatcgca tgttggaaat catgcgtgat
    27721 agccgtttag gcacccacgg cggtctggca ttgatttttg tggtactggc aaagattctg
    27781 gtgttaagcg agttggctct gcgtggcgaa tcgatccttg catcgctggc ggcagcatgt
    27841 gcggtcagcc gcgggactgc cgcattattg atgtatcgtc atcgttatgc gcgggaagaa
    27901 gggcttggca atgtgttcat tggcaaaatt gatgggcgac aaacctgcgt cacactcggc
    27961 ttagccgcta tttttgctgc cgtattattg ccgggtatgc atggtgtggc tgctatggtg
    28021 gtgacgatgg tggcgatttt catcctcggt cagcttctca aacgtacgct gggcgggcaa
    28081 acgggcgata cgctgggagc agccatagag cttggtgaac tggtcttcct gctggcactg
    28141 ctataaagag acgtgagatg caaatacttg ccgatttact gaatacgatc ccagccatcg
    28201 attctaccgc tatgtcgcgt gcacaacggc atattgacgg gttactcaaa cctgttggta
    28261 gcctgggaaa gctggaggtg cttgccatac aactggcagg aatgccgggg ttgaatggca
    28321 taccgcatgt gggcaaaaaa gcggtactgg ttatgtgtgc cgatcacggc gtctgggagg
    28381 aaggggtcgc tatttctcca aaagaagtga cagccataca ggctgaaaat atgacccgtg
    28441 gaacaaccgg cgtgtgtgtg ctggcagaac aagcgggcgc taacgtccac gtaattgatg
    28501 ttggtattga tactgctgag cctatccccg ggcttatcaa catgcgtgtc gcacgaggta
    28561 gcggcaatat tgcttcagct ccggcaatga gtcgccgtca ggctgaaaag ttgcttttgg
    28621 acgtcatatg ttatacgcag gagctggcaa aaaacggtgt cacgctgttt ggtgtaggtg
    28681 aactggggat ggcaaacacg acaccggcag cagcaatagt cagcacaatc actggccggg
    28741 atcctgaaga agtggttggg attggcgcaa acctgccgac agataaactg gctaataaaa
    28801 ttgatgttgt gcgtcgggcg attacgttga atcaaccaaa tccgcaggat ggtgttgatg
    28861 tcctggcaaa agtgggtgga tttgatttgg tcggaatagc tggagtgatg ttaggtgctg
    28921 cttcctgcgg tttacccgtg ttgctggatg gatttctttc ttatgctgct gcgctcgcag
    28981 cctgccagat gtctcctgcg atcaaaccgt atctcattcc ttctcacctg tcggcagaaa
    29041 aaggcgcgcg tatagcgctc tcgcatttgg ggctggagcc ttatctcaat atggagatgc
    29101 gtttaggtga ggggagtggc gcagctctgg cgatgcccat catcgaagct gcctgtgcga
    29161 tatacaacaa catgggcgaa cttgctgcca gtaatattgt tctaccgggg aatacgactt
    29221 ctgatttgaa cagttaaaaa cgaaaagtct gataaaagct atacttaacg gatagctttc
    29281 gcgacatagg aaagggacat gatgcgtcgt gtaaatattc tttgctcatt tgctctgctt
    29341 tttgccagcc atactagcct ggcggtaact tatccattac ctccagaggg tagccgttta
    29401 gtggggcagt cgtttactgt aactgttcct gatcacaata cccagccgct ggagactttt
    29461 gccgcacaat acgggcaagg gttaagtaac atgctggaag cgaacccggg cgctgatgtt
    29521 tttttgccga agtctggctc gcaactcacc attccgcagc aactgatttt gcccgacact
    29581 gttcgtaaag ggattgttgt taacgtcgct gagatgcgtc tttattacta cccaccagac
    29641 agtaatactg tggaagtctt tcctattggt atcggccagg ctgggcgaga aaccccgcgt
    29701 aactgggtga ctaccgttga acgtaaacaa gaagctccaa cctggacgcc aacgccgaac
    29761 actcggcgcg aatatgcgaa acgaggggag agtttgcccg catttgttcc tgcgggcccc
    29821 gataatccca tggggctgta cgcgatttat attggcaggt tgtatgccat ccatggtacc
    29881 aatgccaatt ttggtattgg gctccgggta agtcagggct gtattcgtct gcgcaatgac
    29941 gatatcaaat atctgtttga taatgttcct gttgggacgc gtgtgcaaat tatcgaccag
    30001 ccagtaaaat acaccactga accagatggc tcgaactggc tggaagttca tgagccgttg
    30061 tcgcgcaatc gtgcagaata tgagtctgac cgaaaagtgc cattgccggt aaccccatct
    30121 ttgcgggcgt ttatcaacgg gcaagaagtt gatgtaaatc gcgcaaatgc tgcgttgcaa
    30181 cgtcgatcgg gaatgcctgt gcaaattagt tctggttcaa gacagatgtt ttaagagcgt
    30241 tggtaattag aaagcaaaaa gcctgctaga aagcaggctt ttttgaattt ggctcctctg
    30301 actggactcg aaccagtgac atacggatta acagtccgcc gttctaccga ctgaactaca
    30361 gaggaatcgt gtgaacgggg cgcatattaa tggcagtgca tggtgatgtc aaagggtgaa
    30421 atgcattttt ttgtttgttt gctgtcaatt tcaacaaagt ggtgagcttt caatcttatt
    30481 gggtaatgaa ccatcgtggt gcataccctc cttttatagg gcaggggaac gcgacagctg
    30541 attaaaggag caaatgacga atctatcata atctcttatt tgacagttgg ttagcttgta
    30601 catcaacacc aaaataaaac tggcaagcat cttgcaatct ggttgtaagt aatggcggca
    30661 cttgggccga ttcttaaaaa ccggaggcaa catgaacttc agacgcctga aatacttcgt
    30721 aaaaattgta gatattggta gcctgaccca ggctgctgaa gtattgcata tcgcacaacc
    30781 agcgctcagc cagcaggttg ccacactgga aggtgagtta aatcaacaac ttttgatccg
    30841 tacaaagcgg ggcgttacac caacagacgc cggaaaaatt ctctataccc atgcgcgggc
    30901 cattttacgt cagtgtgaac aggcccaact ggcggtgcat aacgttggtc aggcattatc
    30961 ggggcaagtc tcgattggct ttgcaccagg aaccgctgcg tcatccatca ccatgccctt
    31021 attgcaggcg gttcgcgctg aatttccgga gatcgttatc tatcttcatg aaaatagtgg
    31081 tgcagtgctt aacgaaaaat tgataaatca ccaactcgat atggcggtga tttatgagca
    31141 ttcccctgtg gctggtgtat ccagtcaggc tttgctgaaa gaagatcttt ttctggtagg
    31201 aactcaagat tgcccggggc aaagcgttga tgtgaatgct attgcgcaaa tgaacctctt
    31261 tctccccagt gattacagtg ctattagact tcgtgttgat gaggcttttt ccctacggcg
    31321 actcacggca aaagttattg gtgaaattga gtctattgcc acgcttaccg cagcgattgc
    31381 cagcggcatg ggcgttgcag tattacccga atcggccgcg cgttcgttat gtggcgcagt
    31441 aaatgggtgg atgtcacgca ttaccactcc ttccatgagt ctctctttgt cattaaattt
    31501 acccgccaga gcgaacttat cgccacaggc acaggcagtg aaagagttgt taatgtcagt
    31561 gattagttct ccagtgatgg aaaaaaggca gtggcaattg gtgagctaag tgttatatcg
    31621 ggatggaata agatgcgggt ttttattatt tgttatgccg ggcattagac tttaacaata
    31681 acgggaaatc tgaactgccc ggagtttacc gtgaatttcc aacaactaaa gataatccgc
    31741 gaggctgcac gtcaggatta caacctgaca gaggttgcga atatgctttt tacctcacag
    31801 tccggcgtca gccgtcatat tcgggaactg gaggatgaac ttggcatcga aatatttgtt
    31861 cgacgaggta agcgactgct gggcatgact gaaccgggca aagcattact ggtcattgca
    31921 gaacgtattc tgaatgaagc cagtaatgtt cgtcggcttg cagacctgtt taccaacgat
    31981 acgtctggcg ttctcactat tgcaacgacg catactcagg cacgttatag cttgcctgag
    32041 gtcattaaag cttttcgcga acttttcccg gaggttcggc tggagctaat ccagggtacg
    32101 ccacaggaaa ttgcgacgtt gttgcaaaat ggcgaagctg atattggtat cgccagcgag
    32161 cgtttgagta atgacccgca gctcgtcgcc ttcccgtggt ttcgttggca ccatagtttg
    32221 cttgttccac tcgatcatcc cttgacgcaa attacaccgt tgacgctgga atcaatagcg
    32281 aagtggccgt taatcactta ccgacagggg attacggggc gctcacgtat tgatgacgca
    32341 tttgcccgca aaggtttgct ggcagatatt gtattaagtg cgcaggattc tgatgtcatt
    32401 aaaacctatg ttgctcttgg gcttgggatc ggaatagttg ccgaacaatc cagcggtgaa
    32461 caagaggaaa agaatttaat ccgcctggat acgcggcatc tttttgatgc taatactgtc
    32521 tggttgggac tgaagcgagg acaacttcag cgtaactatg tctggcgctt tctggaactt
    32581 tgtaatgcag gactttcagt tgaggatatc aagcggcagg tgatggaaaa cagtgaagag
    32641 gaaattgatt atcagatata gcaaaaagcc cgctataaaa gcgggctttt cagaaatttg
    32701 gctcctctga ctggactcga accagtgaca tacggattaa cagtccgccg ttctaccgac
    32761 tgaactacag aggaatcgct gttgaggcac atcttaacgg cgaaaaatct tttgtcaaac
    32821 ccgattttta attttcccaa ctgtttgccg tttctatcaa cagtttgttg catttgcaga
    32881 caatttgttg gcgaaaatct ttgcagcttt gctgattctc cctcattatt tgaaatgtgg
    32941 tttcactttc tgcaattaag gtcggctttg aatatctcct ctgctttacg ccaggttgtt
    33001 cacggcactc gctggcacgc taaacgcaag agctacaaag tgttgttctg gcgcgagata
    33061 accccgcttg ctgttcctat tttcatggag aatgcctgtg tcctgttgat gggggttctg
    33121 agcacttttc tggtcagctg gctgggaaaa gatgcgatgg ccggcgtggg attggcggac
    33181 agcttcaata tggtcattat ggcttttttt gctgctatcg atcttggtac tactgtcgtt
    33241 gtggcattta gtctcggtaa gcgggatcga cgacgagcga gggtggcgac gcggcagtca
    33301 ttggtgatca tgacgttgtt tgccgtactg ttggcaacgc ttattcatca ttttggcgag
    33361 caaattattg atttcgtcgc gggtgatgcc acgacagaag ttaaagcact ggcgttgact
    33421 tatctggagc tgacggtact cagttatcca gcagctgcca tcactctaat tggtagcggg
    33481 gcacttcgtg gtgcagggaa tacgaaaata ccgctactga t
//
LOCUS       DACBVI010000039        33461 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.039, whole genome shotgun sequence.
ACCESSION   DACBVI010000039 DACBVI010000000
VERSION     DACBVI010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.039"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            583..1671
                     /locus_tag="IU325_08890"
     CDS             583..1671
                     /locus_tag="IU325_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAP5809141.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGNNSHKYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(1713..2645)
                     /locus_tag="IU325_08895"
     CDS             complement(1713..2645)
                     /locus_tag="IU325_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP5809142.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFSPNGILTGIYQDLLHREN"
     gene            complement(2737..3234)
                     /locus_tag="IU325_08900"
     CDS             complement(2737..3234)
                     /locus_tag="IU325_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HAP5809143.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSTFWASVIMTVPFSAVV
                     VWQGRFGLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            3492..4097
                     /locus_tag="IU325_08905"
     CDS             3492..4097
                     /locus_tag="IU325_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809144.1"
                     /translation="MTIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDQICSNPFLISCLTGDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHMTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            4137..5000
                     /locus_tag="IU325_08910"
     CDS             4137..5000
                     /locus_tag="IU325_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HAP5809145.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDGLFRHGDKVQFDDQGITVGQHLHIEMSHCLRWLSPTLQMAAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYCGDNPFYQALNKELHIKRRAVIQAVNDKQNT
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAEKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            4990..6537
                     /gene="fdrA"
                     /locus_tag="IU325_08915"
     CDS             4990..6537
                     /gene="fdrA"
                     /locus_tag="IU325_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HAP5809146.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKAGADNEQLLVEIEELFNTKAQTGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINAAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFCGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQEHLELHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            6537..7955
                     /locus_tag="IU325_08920"
     CDS             6537..7955
                     /locus_tag="IU325_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HAP5809147.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            8051..9001
                     /locus_tag="IU325_08925"
     CDS             8051..9001
                     /locus_tag="IU325_08925"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP5809148.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSDSQRDELQKANPDWRFVEDGGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            9011..10393
                     /locus_tag="IU325_08930"
     CDS             9011..10393
                     /locus_tag="IU325_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP5809149.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTTHCDAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENNQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQS
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            10692..11102
                     /locus_tag="IU325_08935"
     CDS             10692..11102
                     /locus_tag="IU325_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP5809150.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNVFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTAELARKEGEGDLSL
                     EYWRNEHRRFFQVEGSYSPEMDVIFEEDTLIDIV"
     gene            11356..12339
                     /locus_tag="IU325_08940"
     CDS             11356..12339
                     /locus_tag="IU325_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP5809151.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMENLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            12388..13872
                     /locus_tag="IU325_08945"
     CDS             12388..13872
                     /locus_tag="IU325_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP5809152.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLSFLMTGQEFEYQVRELWQGKSSTPVLEVRHLSRHGEYQNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSEAKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            13865..14836
                     /locus_tag="IU325_08950"
     CDS             13865..14836
                     /locus_tag="IU325_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP5809153.1"
                     /translation="MAELKKRHEFWLALLIVGLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGGIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            14833..15789
                     /locus_tag="IU325_08955"
     CDS             14833..15789
                     /locus_tag="IU325_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP5809154.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGVNILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFTAVSLGLWILLEKTPLGKSIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            15876..16925
                     /gene="yahK"
                     /locus_tag="IU325_08960"
     CDS             15876..16925
                     /gene="yahK"
                     /locus_tag="IU325_08960"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HAP5809155.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPHDVKIEIAYCGVCHSDI
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKYAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQVGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSESKREAAKALG
                     ADEVVNSRNADEMAAHVKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            17168..17983
                     /locus_tag="IU325_08965"
     CDS             17168..17983
                     /locus_tag="IU325_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809156.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNQWISNRVQELGDRYYPVNKQVKIRYTL"
     gene            18396..18641
                     /locus_tag="IU325_08970"
     CDS             18396..18641
                     /locus_tag="IU325_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809157.1"
                     /translation="MAVQLFITLLNQIPLLSSLQSETLPLFGYSGRGRAMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(18658..19329)
                     /locus_tag="IU325_08975"
     CDS             complement(18658..19329)
                     /locus_tag="IU325_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HAP5809158.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPINAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            19476..19751
                     /locus_tag="IU325_08980"
     CDS             19476..19751
                     /locus_tag="IU325_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAP5809159.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(19852..21438)
                     /gene="prpR"
                     /locus_tag="IU325_08985"
     CDS             complement(19852..21438)
                     /gene="prpR"
                     /locus_tag="IU325_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HAP5809160.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLASERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVTDILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKIPSPSLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            21677..22567
                     /gene="prpB"
                     /locus_tag="IU325_08990"
     CDS             21677..22567
                     /gene="prpB"
                     /locus_tag="IU325_08990"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HAP5809161.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARSQAK"
     gene            22612..23781
                     /gene="prpC"
                     /locus_tag="IU325_08995"
     CDS             22612..23781
                     /gene="prpC"
                     /locus_tag="IU325_08995"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HAP5809162.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            23815..25266
                     /gene="prpD"
                     /locus_tag="IU325_09000"
     CDS             23815..25266
                     /gene="prpD"
                     /locus_tag="IU325_09000"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HAP5809163.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGVMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQVAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILDVSLDRARLEQMPVNEYLDLYVI"
     gene            25306..27192
                     /gene="prpE"
                     /locus_tag="IU325_09005"
     CDS             25306..27192
                     /gene="prpE"
                     /locus_tag="IU325_09005"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HAP5809164.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            27429..28688
                     /gene="codB"
                     /locus_tag="IU325_09010"
     CDS             27429..28688
                     /gene="codB"
                     /locus_tag="IU325_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HAP5809165.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAISGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVIPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            28678..29961
                     /gene="codA"
                     /locus_tag="IU325_09015"
     CDS             28678..29961
                     /gene="codA"
                     /locus_tag="IU325_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine deaminase"
                     /protein_id="HAP5809166.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLLDGKISAIDAQSGVMPVTE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLDQPEAIDYKR"
     gene            complement(30001..30900)
                     /gene="cynR"
                     /locus_tag="IU325_09020"
     CDS             complement(30001..30900)
                     /gene="cynR"
                     /locus_tag="IU325_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HAP5809167.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECAVVGGNESR"
     gene            31010..31669
                     /locus_tag="IU325_09025"
     CDS             31010..31669
                     /locus_tag="IU325_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HAP5809168.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHPDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            31700..32170
                     /gene="cynS"
                     /locus_tag="IU325_09030"
     CDS             31700..32170
                     /gene="cynS"
                     /locus_tag="IU325_09030"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HAP5809169.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDERIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            32176..33357
                     /gene="cynX"
                     /locus_tag="IU325_09035"
     CDS             32176..33357
                     /gene="cynX"
                     /locus_tag="IU325_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HAP5809170.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAMTPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LALQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSAQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
BASE COUNT         8029 a         8270 c         9016 g         8146 t
ORIGIN      
        1 ggtttaattg tctgtgtctg atattttatt gattgtttta aatattgttt ttattgtcat
       61 tgttataatg ttggctatct attgctgaat ggattcagcc ttaaaaagca ggttgttaag
      121 ggataggcat agagtaatga cgtgtatgca taaccaacat ctttactcat tatgtcattg
      181 aatgttgacg ctatgtgttt atgaggttga ggtattttca gttgatctgg attgttaaat
      241 tcatataatg cgcctttact catgaatggg cgtcaatacg tagtgggaaa ttataaatat
      301 tgaaatagtc caactacttc tttattacca aaaatgagta tctgaatttt aatattgcat
      361 tcttgcgtga ttatctcctg agtttgactt gtgattacct ttttaaggta tttagcgtaa
      421 ctgtttttga gcgagcatca gaggtaaaga taatcttttt gatagtgatg tgggatgtta
      481 tgcgtatggc atcgctgatg tttatggtta ccccttatgt gtgctcagga atccacaggt
      541 aatcactcat actgaacagc gataaaagat aaaggtgtgt tcatgaattc atgtgatttt
      601 cgtgtttttc tgcaagagtt cggtacaacg gttcatttgt cattgcctgg tagcgtatcc
      661 gagaaagaac gactgctact caagctgctg atgcagggaa tgtctgtaac agaaatatca
      721 cagtacagaa atcgcagtgc aaagaccatt tcacatcaaa agaaacagct ctttgagaaa
      781 ctggggattc agagcgatat tactttctgg cgggatattt tctttcagta caatccggag
      841 atcatatccg ccacgggtaa taatagtcac aaatatatta atgataatca ctatcaccat
      901 atcgtcacgc ctgaagccat cagtctggcg ttggaaaacc acgaattcaa accgtggatc
      961 caaccggttt tctgcgcgca gactggggta ctgacgggct gtgaggtgct tgtccgctgg
     1021 gaacatccac aaacgggaat tatcccaccg gatcagttta ttcctctggc ggagtcatct
     1081 ggtcttatcg tcataatgac tcgccagttg atgaaacaga ctgcggatat tctgatgccg
     1141 gtaaaacatt tgctgccgga caatttccat attggcatca acgtctcggc gggttgtttt
     1201 ttggctgcgg gatttgaaaa agaatgcctg aacctggtta agaaattagg taacgataaa
     1261 atcaaactgg ttctcgagct aacggaacgt aaccctattc cggtaacgcc agaagccaga
     1321 gcgatatttg acagccttca tcagcacaac attacctttg cgctggatga ctttggtacg
     1381 ggttatgcga cctatcgtta cttgcaggcg ttcccggtcg attttattaa gatcgataag
     1441 tcatttgtgc aaatggcgag cgtggacgaa atatccggtc atattgtgga caatattgtc
     1501 gaactggcgc gtaagcctgg tctgagtatc gtggcggaag gggtagaaac ccaggagcag
     1561 gcggatttaa tgatcggcaa aggcgttcac tttttgcagg gctatttgta ctctccgcca
     1621 gtaccgggta ataaatttat ctctgaatgg gtaatgaaag caggtggttg atgtaaaccg
     1681 ctattcacag cgcatcggga ggttggcagc gattaattct cccgatgcag taaatcctga
     1741 tagatccccg ttaatatgcc atttggcgaa aattgttttt taatccactg cgtcacctga
     1801 cccgtagcag aatgctgagt tgcgagtaac atgcgtgaat cctggcgcgg attatggatt
     1861 tgtcgggtaa ccagcaggga ttgcgccatc gcctcacgga ccatgtaatc cggcaaaaaa
     1921 ccaatccctt cacctaaaat ctggcactga catttggtgt tgaaatctgg caccagaatc
     1981 gactcctgcc catgcagcaa ccagcccact tttttattaa tcgtatgcgc ggtgtcctcc
     2041 accatgatat taggatagag acgcagttgg ctttcggcga tcggctccgg cacaaaggct
     2101 aatgggtgat ccggggcgat agcaaaagcc cagcgaattg cgccaatctc cgtgtaatca
     2161 atgccgccgc cgtccagcag tgtgtcgggc gcgccgatag caatattcgc ctgattatta
     2221 ataatcgcat cccagacgcc gttatacact tcggtcgtca cggtaatctg gcaggttgga
     2281 aactgctttt tcagtacctg taataagcgt gcggtgtgtt tgggcgtata aagcagttgg
     2341 ttaatacaaa tgcgcacccg tgcttctata ccctgcgaaa tagtatcgat gctgcgtttg
     2401 atggcgtgaa aatcattcag caggtcggta gctttgcgga aaaaataacg cccggactcc
     2461 gttaactcaa tgcttcgggt actgcgagtg aacagcacca catccagccc tgtttccatt
     2521 cgcttgatgg tgtagctaat ggcggaagtg gttaaaccca actcttctgc ggctttactg
     2581 aagctgccaa aacgcgcagc ggtagtaaat gccaacagat tctcttcggt aaaaattgag
     2641 ttcattaaac cattcccgcc attatctact atgaatttct ccagccatga ctgtagaggt
     2701 atcggttaaa gatagataat catttttgaa taacttttaa tacccgtcgc gtttagtcgt
     2761 cgcttcgctt aatttttgtc cgccatattc tgaaattatc cccacgcagt tacccgccag
     2821 aaaacccagt aatgtcaccg tccagttcat cccgctggca aaaaatagcg tcatgccgag
     2881 aaagcctccg ggaataaaag acaataaacc gaaacgtcct tgccagacca ctaccgccga
     2941 gaagggtaca gtcataatca cgctcgccca gaacgtggac aatccactca ccgaggccag
     3001 ccagccggca gccagcgcgg cgaaaaacgc ccacaccatc ccggaataat taaccgccag
     3061 acttctgata aaccccgatt taccgccgcc tgcggcataa aaactgcaaa aggcgacgaa
     3121 gccaatggtt cctaataatt cccatccctg agagagccct acatgtgaag acactaactg
     3181 ccaaagccct gcacaaatac cgacggtgac acccgttgcc gtgagtccgt tcatcttgtt
     3241 cccctctggt tagacatgga cgaaagctac tcttacatgt gcagactata gagaaataaa
     3301 acctgattga gtgaaagggt atcgggtcaa agaaacaaat attgcactac cgcacactgc
     3361 gaaaagattg ttgaacatca ttcaacaaaa aggcgaaata cggcgaatta aatttaagcg
     3421 ggttctttta tcagattatc gttgctattc tcaattcatc gaaaacagcc acaaaaatca
     3481 ggggaatgtg aatgactata aaaaatctac ctgccgacta tttattggct gcgcaacagg
     3541 gtgatatcga taaagtaaaa acctgccttg cgctgggtgt cgatattaat acctgcgatc
     3601 gtcaggggaa aacggcaatt acgctggcaa gtttatatca gcaatatgct tgcgttcagg
     3661 cattaattga tgccggagcg gatattaata agcaagatca aatatgttca aatccttttt
     3721 taattagctg cctgaccggc gatttaaccc tgttacggat tattttaccg gctaaacctg
     3781 atcttaattg cgtcacccgt tttggcggtg tcggcctgac gcctgcctgt gaaaaaggcc
     3841 atttaagtat tgtaaaagag cttttggcgc atacggagat taacgttaac cagaccaacc
     3901 atgttggctg gacgccgcta ctggaagcaa ttgtgcttaa tgatggcggg attaaacagc
     3961 aggcgattgt ccagttattg ctggaacacg gtgccagccc gcacatgacg gataaatatg
     4021 gcaaaacgcc gctggaactg gcgcgggaac gaggctttga agagattgcg cagttactga
     4081 ttgccgcagg tgcataaacc gggaggcttg ctatcaacac accagaaaga cggtgtgtgt
     4141 gggcgctaac tgcggatgct gattttctgg cgcagcgggg gcaaggtcag gttgaacagg
     4201 tctttgccag agcggtaaat atcgcacttc cggcccgcca gcagttgctg acgctgcttt
     4261 gtgaagagta cgacaatgcg ccaaacagtt gtcggttggc actcactcac tttgatggtc
     4321 tgttccggca tggtgataag gttcagtttg acgatcaagg cattacggtt ggtcaacatc
     4381 ttcatataga gatgagtcat tgcctgcgtt ggctgtcccc aaccttgcaa atggccgctg
     4441 tgaattttca ccttatcgcc tggcaacagt ggcatgacat tattcatcag cacctggggg
     4501 aaaatgaaac cctgtttaat tattgcggcg ataatccgtt ttatcaggcg ttaaataaag
     4561 aattacatat taaacgacgg gcagttattc aggccgtaaa cgataaacaa aataccgcct
     4621 cagcagtcgc cagtatgatg ggcttaggga ttggccttac gccatcagcc gacgattatt
     4681 taacaggtct ggcgcttatt ttatttattt ccggacatcc ggcggaaaaa tacaaagagg
     4741 aattttatct cggtctgcaa cgcggcagaa ataataccac gttattaagt gccataacgc
     4801 tggaagccgc attacaacaa cgctgccggg aaaatattca tcgttttatt cataacatta
     4861 tttatgacat ccctgggaac gcaactcagg caatagaaaa aattaaacat attggctcca
     4921 gttccggctg cgacatgctg tatggcatgg ccgatggttg tgcgctgagc caaacctacg
     4981 gagggaatta tgtcagttaa aatagtcatt aaaccgaata cctattttga ttctgtctcg
     5041 ctgatgtcta tctccacgcg tgcaaataaa ctcgacggcg tcgagcaggc atttgtggcg
     5101 atggcgaccg aaatgaacaa aggcgtgctg aagaatttag ggctgctgac gccggaactg
     5161 gagcaggcga aaaacggcga cctgatgatt gtcatcaacg gcaaagcggg cgcagacaac
     5221 gagcagttgc tggtggagat tgaagaactg ttcaacacca aagctcaaac tggttcacat
     5281 gaagcacgct atgccactat tgccagcgcc aaaaagcata tcccggaaag taacctggcg
     5341 gtgatttcgg tcaacggtct gtttgccgct cgcgaagcgc gtcaggcgct gcaaaacgat
     5401 cttaacgtga tgctgttttc cgataacgtc tcagttgaag atgaactggc gctcaaacag
     5461 ttggcccacg aaaaagggct gctgatgatg gggccagact gcggtacggc gattatcaac
     5521 gccgcggcgc tctgctttgg taacgccgtg cgtcgcggca acatcggcat tgttggcgca
     5581 tctggcaccg gcagccagga actgagcgtc cgtatccatg aattttgcgg cggcgtttcg
     5641 caactgattg gcaccggcgg gcgcgacctg agcgagaaaa tcggcggtct gatgatgctc
     5701 gatgccatcg ggatgctgga aaacgatccg caaacagaaa ttattgtgct tatctccaaa
     5761 ccgcctgcgc ctgcggtggc ccgcaaagtg ctggaacgcg cacgtgcctg ccgcaagccg
     5821 gtggtggtct gcttcctcgg tcgcgttgaa acgccggtgg atgagcaggg gctacagttt
     5881 gctcgcggca gcaaagaagc ggcgctgaaa gcggtaatgc tctccggcgt gaaacaggaa
     5941 catctcgagc tgcatacgct gaaccagccg ttgattgctg acgtgcgcgc tcgcctgcaa
     6001 ccgcaacaga aatacattcg tggcctgttc tgcggcggca cgctgtgcga cgaaacgatg
     6061 ttcgcggtga tggaaaaaca cggcgatgtc tacagcaaca tccagcccga tcctgaattc
     6121 cgcctgcaag atattaaccg cagcatcaaa cacaccttcc tcgactttgg cgatgacgac
     6181 ttcaccaacg gcaagccgca tccaatgatt gaccccacca accgcatcag ccggttgatc
     6241 gaagaagcgc gcgatccaga agtggcggtg atcgtgatgg attttgtgct cggatttgga
     6301 tcgcatgaag atccggtcgg ttccaccatt gaggcgatca aagaggcgaa agcgatcgcc
     6361 gctgccgaag gacgtgagtt gatcattctc gcctatgtgc tgggtaccga tcttgatacg
     6421 ccatcattag aacaacaaag ccagatgctg cttgatgctg gggtaattct ggccagcagt
     6481 agcaccaata ccggattgct ggcgcgtgaa tttatctgca aaggggagga agcctgatga
     6541 gccagtcact gtttagccaa ccattgaacg ttattaacgt cggcatcgcc atgtttagcg
     6601 atgatctgaa aaagcagcat gtagaagtga cccaactcga ctggacgccg ccggggcagg
     6661 gcaatatgca ggtggtgcag gcgctggaca acattgccga ttccccgctg gcagacaaaa
     6721 tcgccgccgc taaccagcag gctttggagc gcattatcca gtcgcatccg gtgctgattg
     6781 gttttgatca ggcgattaac gtggtgccgg gtatgacgcc gaaaaccatt cttcacgccg
     6841 gaccgccgat cacctgggaa aaaatgtgtg gcgcgatgaa aggcgcggtc accggagcgc
     6901 tggtgttcga agggctggcg aaagatctcg atgaggcggc tgaactggcg gcctccgggg
     6961 agatcacctt ctcgccgtgc cacgaacatg actgcgtggg atcgatggcg ggtgttacct
     7021 cggcctcgat gtttatgcac atcgtgaaaa acaaaaccta cggcaacatc gcttacacca
     7081 atatgagcga gcagatggca aagattttgc gtatgggcgc taacgaccag agcgtgatcg
     7141 accgactcaa ctggatgcgc gatgtgcagg gacccatgct gcgcgacgcg atgaaaatta
     7201 tcggcgaaat cgatctgcgc ttaatgctgg cgcaggcgct gcatatgggc gatgagtgcc
     7261 ataaccgcaa taacgccggg acgacgctgc tgattcaggc gctgacgccg gggattattc
     7321 aggcgggcta ctccgtcgag caacagcgcg aagtgtttga gtttgtcgcc agcagcgact
     7381 acttctccgg cccgacgtgg atggcgatgt gtaaagcggc gatggatgcg gcgcacggca
     7441 tcgaatacag caccgtggtg accacgatgg cgcgtaacgg cgtcgagttc ggcctgcggg
     7501 tcagcggatt accggggcag tggtttaccg gtccggcgca gcaggtgatc ggcccgatgt
     7561 ttgccggtta taagccggaa gattctggat tggatattgg cgacagcgcc atcactgaaa
     7621 cctacggcat tggcggattt gccatggcga ccgcgcctgc tatcgtcgcg ctggtgggcg
     7681 gtacggtgga agaggctatc gatttctccc gccagatgcg tgaaatcact ctcggtgaaa
     7741 accccaacgt caccattccg ctgctcggtt ttatgggcgt gccgtcggca atcgacatca
     7801 cccgcgtggg cagcagcggc attttgccgg tgatcaatac cgccattgcc cataaagatg
     7861 cgggcgttgg catgattggc gcgggcattg tgcatccacc attcgcctgc ttcgagaaag
     7921 ccattcttgg ctggtgcgaa cgttacggcg tctgaccctc caactgcacc ttacgatccc
     7981 ttctcccttc tggggagagg ggcaacctca ctctcataac cccatcaccc tctgtagcgg
     8041 acataacaac atgaaagaac ttgtggtcgt tgccattggt ggcaacagca ttatcaaaga
     8101 taacgccagc cagtcgattg agcatcaggc ggaggcggtg aaagccgtcg ccgatacggt
     8161 gctggaaatg ctggcttccg attacgacat tgtgctgacc cacggcaacg ggccgcaggt
     8221 cgggctggat ttacgccgtg cggagattgc ccacgagcgc gaagggctgc ccttaacgcc
     8281 gctggcaaac tgtgtggcgg atacgcaggg cggcattggc tatctgatcc agcaggcgct
     8341 gaacaaccgg ctggcccgcc acggcgagaa aaaagccgtc accgtggtga ctcaggtgga
     8401 agtggataaa aacgatccgg gatttgccca tcccaccaag cccatcggcg cattctttag
     8461 tgacagccag cgtgacgaat tacaaaaggc aaaccctgac tggcgttttg ttgaagatgg
     8521 cggacggggc tatcgccgcg tagtcgcctc gccggaaccg aaacgtattg tcgaagcacc
     8581 ggccattaag gcgctgatcc aacaaggctt tgtggtgatt ggcgcgggcg gcggcggaat
     8641 tcctgtggtg cgcaccgaag cgggagatta ccaaagcgtg gacgcggtta tcgacaaaga
     8701 tctctctacc gcgctactgg ctcgcgaaat tcacgccgac attctggtga tcaccacggg
     8761 cgtggaaaaa gtgtgtattc attttggcaa accgcagcag caggcgctcg atcgggtgga
     8821 tattgccacc atgacccgct atatgcagga agggcatttt ccgcctggca gcatgttgcc
     8881 aaaaatcatc gccagcctga cgttcctgga acagggcggc aaagaagtga ttatcaccac
     8941 gccggaatgc ctgcctgcgg cgctgcgcgg tgaaacgggc acccatatta ttaaaacgta
     9001 aggacgtaag atgaaagaaa gcaatagccg ccgtgagttt ctgagccaga gcggtaagat
     9061 ggtcaccgcc gccgcgctgt ttggtacctc tgtgccgctc gcccatgcgg cagtctctgg
     9121 cacaacccac tgcgacgcga ataacaccat gaaaatcact gacccgcact actatctcga
     9181 caacgtgttg ctggaaaccg gttttgacta cgaaaatggc gtggcggtgc agacccgcac
     9241 ggcgcgccag actgtggaaa ttcagaacgg caaaattgtt gcactgcgcg agaacaatca
     9301 gcatccggac gccacgctgc cgcactatga cgctggcggt aagctgatgc tgcccaccac
     9361 ccgcgacatg catattcatc tcgacaaaac cttctacggc gggccgtggc gctcgctcaa
     9421 tcgtccggca ggcaccacca tccaggacat gatcaaactc gagcagaaaa tgctgccgga
     9481 gttgcaatcg tacactcagg aaagggcgga aaaactgatt gatttattgc agtcgaaagg
     9541 cactaccatt gcccgcagcc actgcaatat cgaaccggtt tccggcctga aaaatctgca
     9601 aaatttgcag gcggttctgg cgcgacgtca ggcgggcttc gagtgtgaaa ttgtcgcctt
     9661 cccgcagcac ggtttgctgc tgtcgaaatc agaaccctta atgcgcgaag cgatgcaggc
     9721 gggggcgcat tacgtcggcg ggctggaccc gaccagtgtt gatggcgcga tggaaaaatc
     9781 cctcgacacc atgttccaga ttgcgctgga ctacgacaaa ggcgttgata ttcacctgca
     9841 cgaaaccact ccggcaggcg tggcagccat caattatatg gttgaaacgg tggagaaaac
     9901 gccgcaactg aagggcaagc tgaccatcag ccacgccttt gcgctggcaa cgctcaatga
     9961 gcaacaggta gatgaactgg cgcaccggat ggcggcgcag caaatttcta tcgcctcgac
    10021 ggtgccgatt ggcacgctgc atatgccgct caaacagttg cacgacaaag gcgtaaaagt
    10081 gatgaccggc accgacagcg ttatcgacca ctggtcgcct tacggcctgg gcgacatgct
    10141 ggaaaaagcc aatctgtacg cgcagctcta tattcgtcct aacgaacaga acctctcccg
    10201 ctcgctgttt ttagccactg gcgatgtatt gccgctcaac gaaaaaggcg agcgcgtgtg
    10261 gcccaaagcg caggatgatg ccagctttgt gctggtggac gcctcctgtt ccgccgaggc
    10321 ggtggcgcgt atctcgccga gaaccgcaac gttccataaa gggcaactgg tgtgggggag
    10381 tgtggcaggt tgatgcgcat ttgaaacgcc tgcaacggtg aatagtaaga gatttaagcc
    10441 ccagggagtg gggcgggaat ggtttgttaa tgaatgatcc tgaactttat aatctcccgg
    10501 cataatttcc aggggattat ttgagagaaa tacacaaaag tgagttttaa caaaggtatt
    10561 tatcaagcca taaatgtgac ctgcttcgga taaaaaagat taaaaataga aaaaccaatg
    10621 atactcttgc tgatggttat caacagtaat taagatgtga tttatttttc cgtaatcgta
    10681 aaaatattgc catgaccata aaagaaaaat tctcacaaaa atatccccat gcttcctttt
    10741 gtacttttgg cgattcagca gcgttggccg atcacctcgc aacgttgatt gcgactggtg
    10801 ttaaaacagc ttcctgtggt tcgctggcgg gatgtattga agacaacgta tttccgatga
    10861 ttggtgagta taagattgta gaaaatagcc gtggtgaacc tgtatgtgtt atcagagtga
    10921 ttggcctgca tttactgcgc ttttctgatg tcaccgcaga gcttgcccgt aaggaaggtg
    10981 aaggtgacct cagtcttgaa tattggcgta atgaacaccg tcgttttttc caggtagaag
    11041 gaagctattc accagaaatg gacgttatct ttgaagaaga tacccttatt gacattgtgt
    11101 agataactat ttatctacgc gaaataaact cacaaacata aagtatcgct aattttgttt
    11161 attttgctat tttttacgtg atgattgtca cacttattgt tactgcccgt tttgtccata
    11221 tttataacca aatttttaat agctgcctga aatagggctg tttattctgc ataaaaatat
    11281 tcgaaaattc ttattctctc cagcgcgcta atttgggcga acacttcctg actaccctgc
    11341 aatgaggctg aaatgatgaa taaacgtttt gttatcaata tggtgtcttc gctgttatta
    11401 ggcgctgcgc taatttctgc gccgctccag gcagcggaaa aagtcgtggt gaatatctct
    11461 aaagtcgatg gtatgccgtg gtttaatcgc atgggcgaag gtgtggttga ggcgggtaaa
    11521 gcctttggtg ttaatgcgtc acaggtgggg ccttcaagta ctgatgctcc gcaacaagtt
    11581 aaaattattg aggatttaat cgcccgtaag gttaatgcaa ttacaattgt gccaaacgat
    11641 gccaacgtgc tggaaccggt attcaaaaaa gcgcgagatg ccggaattgt ggttctgacc
    11701 aacgaatccc ccggtcagcc aagtgctaac tgggatatcg aaatcattga taacgaaaaa
    11761 tttgccgctg aatatgttga gcatatggcg aaacgcatgg gtggcaaggg cggctatgtg
    11821 atttacgtgg gtagtttgac cgtgccgcag cataacttat gggctgactt gctggtgaaa
    11881 taccagaaag agcattaccc ggatatgcat gaagttaccc gccgcatgcc ggttgccgag
    11941 agtgtggatg actcacgtcg taccacactg gacctcatga aaacctatcc tgacttgaaa
    12001 gcggttgtct cctttggttc aaacggtccg attggcgcgg gacgtgcagt gaaagagaaa
    12061 cgggcgaaaa ataaagtcgc agtctatggc atgatgattc cttcacaggc ggcatcactg
    12121 attaaaagtg gtgatatcac cgaagggatt acttacgatc cggcctctgc aggttacgca
    12181 ctcgcggccg tcgccagcac gttgctgaag ggtgaagaga tcaaacctgg attagaaatg
    12241 gagaatctcg gtaaggccga cgtagatatg gataagcgaa ttatccgttt ccataaggta
    12301 ttactggtca acaaagacaa tatcgactca ctttattaat ccatttcttc ggggcgaaag
    12361 ccccgattct tattgccagg ggatgctatg gaaaccttcc tttcccttcg tcatatcaac
    12421 aaaacgtttc atgccacgcg agcgctgcgc gatgtttctc tcgactttat gtcgggtgaa
    12481 gtgcactgcc ttgccgggca aaacggctgt ggtaaatcga cgttgattaa aattatgtcc
    12541 ggtgtttatc gcccggatga gggagcggaa attacgcttg gtgggaaaaa ctggtcaaag
    12601 ctgacacccg ccgcttcggt ggcgcagggg attcaggtga tttatcagga cctctcttta
    12661 tttcctaacc tgagtgtctg ggagaatatc gccgtgaatc actatcacca cggtctgttt
    12721 gttaaccgac gtcgtctgcg tgaggtggcg caggcggcaa tgaccagtat taacgtcaca
    12781 ttgcctctgg atacgctcgt ttctgagctt tcaattgccc gctgtcagct ggtggcaatt
    12841 tgccgtgcac tggcgcagga cgcacggttg atcgtgatgg atgaaccaac cgcttcgctg
    12901 acccatcagg aagtgcaagg gttattacag gtggtgcatc aattgcggga acgcgggatc
    12961 tgcgtggtct tcgtcagtca tcgtctggaa gaggtgatgg aagtttccga ccgtatttca
    13021 gtgttgaaag atggtgagct ggtcgggacc tttccggccg cagagatgac cacaaaacaa
    13081 ctcagttttc tgatgaccgg ccaggagttc gaatatcagg tgcgagagtt gtggcaggga
    13141 aaatccagca ctccggtgct ggaggtgcgt catttaagtc gtcatgggga atatcaaaat
    13201 attaacttgc gggtggaagc gggcgaagtg gtgtcgattg ttggcctgct tggtgcgggg
    13261 cgcacggaat tatgtctgag tctgtttggt atgacccgac cggatgctgg cgagatcctc
    13321 atcaacggtc agccggttac actgcatagt aaccaggacg ccatccgcca cggtattggt
    13381 tatgtttcgg aagatcgcat gtcgcgcggt ttggtgatgg cgcaatctat tgaagacaac
    13441 atcattagca ctgtttttca caaggtaaaa gatcgcttcg gctttctgag tgaagctaaa
    13501 gtgcgtgacc tggtggacag gctaatcaag gcattgacca tcaaagtttc tgatcctcat
    13561 ctcccggtga atacgctttc tgggggtaac gcgcagcgag tatcgatcgc caaatggctg
    13621 gcgattgggc cacgactgtt gattctcgat agcccgacag tcggtgttga tatcgccaac
    13681 aaagcaggaa tatacggcat tatcagcgat ctggcggctc atggcattgc ggtattgatg
    13741 atctgcgatg aaattgaaga agcgtggtat caaagccacc ggattttggt aatgcaaaaa
    13801 ggtcagatca cccatagctt cctgcctgac agcagttctc aggccaggat tgcggaggtg
    13861 gtaaatggct gaactgaaaa aacgtcatga attctggctg gcattactga ttgtggggct
    13921 gtttgtcggc cttgcctggc gcagcgacga gtttctgaca ttcggtaatt tgtacgatct
    13981 cgccaataac tatgccatgt tgactattct cgcctgtggt ttgtttgtgg tgttaatttc
    14041 cggtgggatt gatatttcgt ttccggcaat gaccatcatt gcgcaatacg gcatggtgct
    14101 gctgctgcaa aaaattggtg gcaactttgc tgtcgcgttt gccctggcgg gcggcatcgg
    14161 cattttactt ggcttaatta acgccttact ggttaatcgc ctacgggtgc cttctatcat
    14221 catcactatc tcgacgctga atatttttta tggcctgctg ttatggttga gtaaaggtgt
    14281 gtggctgtac gactttccgc cgtggtttga gcagggggta atgttgttca agtacaccga
    14341 tgctgatggc tatgactatg gccttggtct gccgctgatc gccatgatta cggtggtgct
    14401 gctgacagcg tttatcatga atttcaccag tgtagggcgc aaaatttatg cccttggcgg
    14461 gaaccgtgaa tccgccagtc gcatcggctt tagcgtgctg aaactgcaac ttttcgtcta
    14521 cggctatatg ggattgatgt ctggtgctgc gggtgtagtg cagtcgtgga cggtgatgac
    14581 tgtcgccccc gattctcttc tgggttatga gctgacagta ctggctgcgg tggtgcttgg
    14641 cggcactagt ttgctcggcg ggcgcggcac gttaaccggt actttgctcg gcgtggtgtt
    14701 gttggcagtg atgcaaaacg ggctaaattt attgggagtc tcgtcttact ggcaaacatt
    14761 gatcaccggc atcatcatcg ttgccagcat tagtgccacg gcgtggagtc agcatcagaa
    14821 ccggagtctg ctatgaaaaa atcgtggcgt aacaacgttg agttttactt gatcggcctg
    14881 ctggtgctaa ctgttgcagc cttcagcatt actatgccgg agattttctg gtcgataagt
    14941 aatttccagt ccgtcgcttc gcaaatgccc gtgctgggca ttctggcgtt ggctatggcg
    15001 gtgaccatgc tttgcggtgg cattaacttg tcaattatag ccactgctaa cgcctgttca
    15061 ctggtgatgg cgtgggtggc gactcagtat ccaccgggta ttgctacggt tgtcgctacg
    15121 ctgctggcgg gcgctggcgc ggcggtgata atcggtttat gtaacggcgt attgattgct
    15181 ggtattcgag tgtcgccaat tcttgcaacg cttgggatga tgacgttgct caaaggggta
    15241 aatattctgg tgacaggcgg tagcgccatt gccaattatc cctcatgggt gctgtggttg
    15301 aatcatgcac agtggttcgg tattccgcta ccgatgtggt tgtttaccgc agtgtcgcta
    15361 gggctgtgga tattgctgga gaaaacgccg ctcggtaaat ccatttatct gattggttct
    15421 aacgaacgag caacacttta ctccggcatt aacactcgtc gggtgctgat ttgggtgtat
    15481 gtcatttccg ccttgctttg tgcggtagcg gcatttttga tgatgtcgaa actcaactcg
    15541 gctaaggcct cttatggcga atcgtacttg ctggtgtcaa tcctcgcggc agtactcggc
    15601 ggggttaatc cggacggcgg cagcgggcgg ataattggta tggtgctggc gctgtttctt
    15661 ctgcaaatta ttgaaagcgg cttcaatatt ctcggtataa gcccctacct gacgatggcc
    15721 ttatggggaa cgttgctgct ctgtttcatt caggctcgcg gcatgttggg gctggatcgg
    15781 gtggtttaaa tattggctat attcaatgga cgcgttttgc cgcgatgaca tatcaggcat
    15841 cgccaaatac acatagctaa tcaggagtaa acacaatgaa gatcaaagct gttggtgcat
    15901 attccgctaa acaaccactt gaaccgatgg atatcacccg gcgtgaaccg ggaccgcatg
    15961 atgtcaaaat tgaaatcgct tactgtggcg tctgccattc cgatatccac caggtccgtt
    16021 ccgagtgggc ggggacggtt tatccctgcg tgccgggcca tgaaattgta gggcgcgtgg
    16081 tagctgttgg tgatcaggta gaaaaatatg cgccgggcga tctggtcggt gtcggctgta
    16141 ttgtcgacag ttgtaaacat tgcgaagagt gtgaagacgg gctggaaaac tactgtgatc
    16201 acatgaccgg cacctataac tcgccgacgc cggacgaacc gggccatact ctgggcggct
    16261 actcacaaca gatcgtcgtt catgagcgat atgttctgcg tattcgtcac ccgcaagagc
    16321 agctggcggc ggtggcacct ttgttgtgtg cagggatcac cacgtattcg ccgctacgtc
    16381 actggcaggt cggtccgggt aaaaaagtgg gcgtggtcgg catcggcggt ctgggacata
    16441 tggggattaa gctggcccac gcgatggggg cgcatgtggt ggcatttacc acttctgagt
    16501 caaaacgcga agcggcaaaa gccctggggg ccgatgaagt tgttaactca cgcaatgccg
    16561 atgagatggc ggctcatgtg aagagtttcg atttcatttt gaatacagta gctgcgccac
    16621 ataatctcga cgattttacc accttgctga agcgtgatgg caccatgacg ctggttggtg
    16681 cgcctgcgac accgcataaa tcgccggaag ttttcaacct gatcatgaaa cgccgtgcga
    16741 tagccggttc tatgattggc ggcattccag aaactcagga gatgctcgat ttttgcgccg
    16801 aacatggcat cgtggctgat atagagatga ttcgggccga tcaaattaat gaagcctatg
    16861 agcgaatgct gcgaggagat gtgaaatatc gttttgttat cgataatcgc acactaacag
    16921 actgaaaaaa ttaataaata ccctgtggtt taacatatta acttcgctct ccacttaact
    16981 ttttagttaa ggagagcgaa ataatatcaa agtagcagta aaacctataa cgtaaattca
    17041 aattgttaaa ttaactccct ccagtacaca atacttcaca cgttagttat gagcgatttc
    17101 tgatagtgcc tggtttaatc agagctttat tatctgcgac gtttattttt atttaagaga
    17161 gatcgcgatg atatcattaa aagctccgca caataatttg atgccatata cgcaacaaag
    17221 catacttaat acggttaaaa acaatcagtt accagaggat atcaaaagct ccctggtttc
    17281 ctgtgtcgat atattcaagg ttttgattaa acaatattat gattatcctt atgattgtcg
    17341 tgatgattta gtcgatgacg acaaactcat tcatctcatg gctgccgtac gagactgtga
    17401 gtggtcagac gataacgcac tcaccataaa tgtgcagttt aatgatttcc ctgggtttta
    17461 tgactggatg gattaccctg atcacccggt taagtttgtt tttcgcatac ttgaaaatca
    17521 aaaagggaca gtttgggtat atgaccagga cgatgcgttt cttgatatta aagcaaatgt
    17581 gcaggctgga cgctttaccg ggcttaagaa attagtgcag tttatcgatt cagtgcgaac
    17641 agattgcaaa tgtattcttc ttgaacacca tatgcctttg ctgagacttt tccctaaagg
    17701 gaaagagtgt atgcatgtgg aaaaatggct tcgcgaaatg tcgtctatcc cggagaccga
    17761 cgcgcccatt aagcaggcgc ttgcgcatgg attattatta cacttaaaaa atatctaccc
    17821 tgtttttcct gaatcactgg tgatgttatt gctctctgtt ctggacgtaa aaacatacag
    17881 agatgatgcg cgattaaatc aatggatatc taatagagtc caggaattag gtgacagata
    17941 ttatcctgta aataagcaag ttaaaatccg ctacactctt tagttcagaa agggttccac
    18001 ctgtgagttg ttattccacc agagagtgaa tcgcaaagca ggtaatcatt tcatctgtat
    18061 atccgtatgc caaatggcat tccaccgcct ttttcgccga tattttgttg tgatcggcga
    18121 cgcttcgctt aaaaaagcat cagcagtggt ttcgcagcta tacggtgtat aaaaaatgat
    18181 ctcatgcaga tgttttgtga atgtgtgggt tgacattcat atgaaaaaaa tcataattcc
    18241 atcatgtttg tgtggggtct tttctgtatc ttacgcatcg cactcaagcc tgacagaaaa
    18301 tatgctgtaa ggctcatatc aaaaaccgcc attagctcat caggaagagc agacgacaac
    18361 caggattgtt gtatggtacg gggttcgagg cctcgatggc ggtccaactt ttcataactc
    18421 ttctgaatca gatcccctta ttatcatccc tgcaaagtga aacattgcca ttgtttggct
    18481 attcaggacg gggcagagca atgaaaaaag cccacacagg ggagagtggg ctgaaatggg
    18541 aagctaaaga ctcaagtaaa cttatcggaa ataaggacca cgcattacgg gggctatcat
    18601 cgccgatggt cgggattcgg caaatcaggt tgattacgta gagagtaaat tatctgctca
    18661 ccgctgcgtc accccttcgt aaatcaggcg tagcgcgaat acaccaataa ttgcaccaat
    18721 aacccgactg gcaacgcgtt gcatacgccc ataagcacga cgcactgcgg gcaaagaaaa
    18781 cgcctgacta agaaaaacgc gccagataat tgatgcgagc acaatccccg cccaggccat
    18841 taaacgtgcc catgttggtg tttcggcatt taatgttact gagaaaatac tgataaaaaa
    18901 taaaacggtt tgcgggttag agagatcggt aattaatccg cggcgaaaaa agacatacca
    18961 gggggcgtta atcggttgtt gtagtgtgct catttgcggt gttgactggc ggcgcatgct
    19021 gcaccacgca aaccataaga gataagcgcc gccgacgatt ctgataagcg aaaaaatctc
    19081 ctcacactgc gtaattagcg ttgcaagacc aaacaaaccc aacccggaat aaaatgcatc
    19141 gcccagcgcc acgcccagcc cggtcagcac ccctgcgcgt cgaccggaag ccaggctggt
    19201 ttgtactacc acaaagagat tggctcccgg attaaaaaaa gtaatcacga acagtcctac
    19261 ggtcaggtaa acggcgtgca aaggatccat agtgatttca tccataaata agtgaagtaa
    19321 cttcatcata tttctaccaa aaataatcgg gtgcgagaga gatcacaaag tgtcttattt
    19381 ccggttactg gcgtttatgc cctgactgaa ctaattatta atcaacccaa taatgtgggt
    19441 gggtaatagt gtgataacaa ctctggagcc gtaatatgaa aataatctct aaaatgttag
    19501 tcggtgcgtt agcgtttgcc gttaccaatg tctatgccgc agaattaatg accaaagcgg
    19561 aatttgaaaa ggttgaatcg caatatgaaa aaataggtga tatttcaacc agtaatgaaa
    19621 tgtcgaccgc agatgcgaaa gaagatttga tcaaaaaagc cgatgaaaaa ggggctgatg
    19681 tgttggtgct gacctctggt caaactgaca ataagattca cggcacggca aatatttata
    19741 agaagaagta attctgaatc ctatgtaaac atccccgatg cgtaagttta tcggtgatca
    19801 tcatctattg aaatttatgc cggataaagc gttcgcgctg cattcggcag ttcagctttt
    19861 cagccgccgc cagaacgtcg tccggctgat acctaaataa ttcgccgctg ctgttttatc
    19921 gccattaaat ttctccagtg cctgttgtgg tgtcagcaag gatggggagg gaattttcgc
    19981 tgactcgcgc gccagctccg gcagtagcag ctgcaaaaac tgcggcgtta aatccggcgt
    20041 cggttccaca cttaaaaaca gcgccagtcg ctccatcata ttgcgcagtt cacgaatatt
    20101 gcccggccag tcgtagtgca ccagcacggt ttcgcttgcc tgtaatccct ggcgtaatgc
    20161 ggcagaaaac ggggcggaga gcgccgccag agacactttc aaaaagcttt ccgccagcgg
    20221 cagaatatcc gtcacccgct cgcgcagtgg tggcaattgc agacgcaaaa tactcagccg
    20281 ataaaacaga tcacggcgaa actgcccttg ccgcatatct tcttccagat tgcagtgagt
    20341 ggcgctaatg acccgcacat ctaccggaac aggctgatgc ccgccgacgc gggtgacctc
    20401 tttttcttcc agcacccgta acaggcgagt ctgcaaaggt agcggcattt cgccaatctc
    20461 atccagaaac agcgtaccgc cgtgggcaat ttcgaacagc ccggcgcgac ctccacgtcg
    20521 cgagccggtg aacgcccctt cctcatagcc aaacagctct gcttccagca gcgattcggc
    20581 aatcgccccg cagttgacgg caacaaacgg atgcgacttt ttgccctgtc gcgcatcgtg
    20641 gcgggcaaaa tattcccgat gaatcgcctg ggccgccagc tctttgcccg tccccgtttc
    20701 cccctcaatc aacaccgccg cactggagcg ggcatacagc aaaatagtct gccgtacctg
    20761 ttccatctgt ggtgattgac cgagcatatc gcccagcacg taacgagtac gcagggcgtt
    20821 gcgggtggca tcgtgagtgt tatggcgtaa cgacatgcgc gtcatatcca gcgcatcgct
    20881 gaatgcctgg cgcacggtgg cggcggaata gataaaaatt ccggtcattc cggcttcttc
    20941 tgccaaatcg gtaatcagcc ccgcgccgac caccgcttcg gtgccgttag ctttcagctc
    21001 gttaatctgc ccgcgcgcgt cttcttcggt aatgtagcta cgttggtcga ggcgcaaatt
    21061 aaaggttttt tgaaacgcca ccagcgccgg aatagtttcc tgataagtca caacgccgat
    21121 agaagaggtg agttttccgg cttttgccag tgcctgtaac acatcgtagc cgctcggttt
    21181 aatcaaaata actggcactg acaggcggct tttcaggtac gcgccgttag agccagcagc
    21241 gatgatggcg tcgcagcgtt cgctagccag tttcttgcgg atgtaggtca ccgctttttc
    21301 aaagccaagc tggatagggg taatgttcgc cagatgatca aactcgaggc tgatatcgcg
    21361 aaacagctcg aacaggcgcg ttacagatac cgtccagata accggtttgt cgtcattcag
    21421 ccgtggtgga tgtgccatag cgcaccgcaa agttaagaaa ccgaatattg ggtttagtct
    21481 tgtttcataa ttgttgcaat gaaacgcggt gaaacattgc ctgaaacgtt aactgaaacg
    21541 catatttgcg gattacttca tgactatatt gctaacaaat tgaaattaaa catttaattt
    21601 tattaaggca attgtggcac accccttgct ttgtgtttat caacgcaaat aacaagttga
    21661 taacaaagga tgggctatgt ctctacactc tccaggtaaa gcgtttcgcg ctgcactgac
    21721 taaagaaaat ccattgcaga ttgttggcac cattaacgcc aaccatgcgc tgctggcgca
    21781 gcgtgccgga tatcaggcga tttatctttc cggcggtggc gtggcggcag gttcgctggg
    21841 gctgccggat ctcggtattt ctacccttga tgacgtgctg acagatattc gccgtatcac
    21901 cgacgtttgt tcgctgccgc tgctggtgga tgcggatatc ggttttggtt cttctgcttt
    21961 taacgtggcg cgcaccgtga aatcgatgat taaagccggt gcggcaggac tgcatattga
    22021 agatcaggtt ggtgcgaaac gctgcggtca tcgtccgaac aaagcgatcg tctcgaaaga
    22081 ggagatggtg gatcggatcc gcgcggcggt ggatgcgaaa accgatcctg attttgtgat
    22141 catggcgcgc accgatgcgc tggcggtaga ggggctggat gcggcgatcg agcgtgcgca
    22201 ggcctatgtt gaagcgggtg ccgagatgtt gttcccggag gcgattaccg aactcgccat
    22261 gtaccgccag tttgccgatg cggtgcaggt gccgatcctc gccaacatca ccgaatttgg
    22321 cgcaacaccg ctgtttacca ccgacgaatt acgcagcgcc catgttgcaa tggcgctgta
    22381 cccgctttca gcgtttcgcg ccatgaaccg cgccgccgaa catgtctaca acgtcctgcg
    22441 tcaggaaggc acgcagaaaa gtgtcatcga caccatgcaa acccgcaacg agctgtacga
    22501 aagcatcaac tactaccagt acgaagagaa gctcgacgac ctgtttgccc gtagccaggc
    22561 gaaataaaac acaacgaaaa atatctcgac cctacaaatg acgaggacaa catgagcgac
    22621 acaacgatcc tgcaaaacag tacccatgtc attaaaccga aaaaatctgt ggcactatcc
    22681 ggcgttccgg cgggcaatac ggcgctctgc accgtgggta aaagcggcaa cgacctgcat
    22741 taccgcggct acgatattct tgatctggcg gaacattgcg aatttgaaga agtggcgcac
    22801 ctgctgatcc acggcaaact gccgacccgt gacgaactcg ccgcctacaa aacgaaactg
    22861 aaagccctgc gcggtttacc agctaacgtg cgtaccgtgc tggaagcctt acccgcggcg
    22921 tcgcacccga tggatgttat gcgcaccggc gtctccgcgc tcggttgcac gctgccagaa
    22981 aaagagggtc ataccgtctc tggtgcgcgg gatattgccg acaaactgtt ggcgtcgctt
    23041 agctcgattc ttctctactg gtatcactac agccacaacg gcgaacgcat ccagccagaa
    23101 accgatgacg attccatcgg cggtcacttc ctgcatctgc tgcatggcga aaagccgtcg
    23161 caaagctggg aaaaggcgat gcatatttcg ctggtgctgt acgccgagca cgagtttaac
    23221 gcctccactt ttaccagccg ggtgattgcg ggcactggct ctgatatgta ttccgcgatt
    23281 attggcgcga ttggcgcact gcgcgggcca aaacacggcg gggcgaatga agtgtcgctg
    23341 gagatccagc aacgctacga aacaccggac gaagccgaag ccgatatccg caagcgcgtg
    23401 gaaaacaaag aagtggtcat tggttttggt catccggttt acaccatcgc cgacccgcgt
    23461 catcaggtga tcaaacgcgt ggcgaagcag ctctcgcagg aaggcggttc gctgaagatg
    23521 tacaacatcg ccgatcgcct ggaaacggtg atgtgggaga gcaaaaagat gttccccaat
    23581 ctcgactggt tctccgctgt ttcctacaac atgatgggcg ttcccaccga gatgttcaca
    23641 ccactgtttg ttatcgcccg cgtcaccggc tgggcggcgc acattatcga acaacgtcag
    23701 gacaacaaaa ttatccgtcc ttccgccaat tatgttggac cggaagaccg ccagtttgtc
    23761 gcgctggata agcgccagta aacctctacg aataacaata aggaaacgta cccaatgtca
    23821 gctcaaatca acaacatccg cccggaattt gatcgtgaaa tcgttgatat cgtcgattac
    23881 gtgatgaact acgaaattag ctctaaagtg gcctacgaca ccgcacatta ctgcctgctc
    23941 gacactctcg gctgcggtct ggaagctctc gaatacccgg cctgtaaaaa actgctgggg
    24001 ccaattgttc ctggcactgt cgtccctaac ggcgtgcgcg tccccggaac tcagttccag
    24061 ctcgaccccg tccaggcggc atttaacatc ggcgtgatga tccgttggct cgatttcaac
    24121 gatacctggc tggcggcgga gtggggccat ccttccgaca acctcggcgg cattctggca
    24181 acggcggact ggctttcgcg taacgcggtc gctagcggca aagcgccgtt gaccatgaag
    24241 caggtgctga ccgcaatgat caaagcccat gaaattcagg gctgcatcgc gctggaaaac
    24301 tcctttaacc gcgtcggcct cgaccacgtt ctgttagtga aagtggcttc caccgccgtg
    24361 gtcgccgaaa tgctcggcct gacccgcgag gaaattctca acgccgtttc gctggcgtgg
    24421 gtggacggtc agtcgctgcg cacctatcgc catgcgccga acaccggcac gcgtaaatcc
    24481 tgggcggcgg gcgatgccac ttcccgcgcg gtacgtctgg cactgatggc gaaaacgggc
    24541 gaaatgggtt acccgtcagc cctgactgcg cctgtgtggg gcttctacga cgtctccttc
    24601 aaaggtgaat cgttccgctt ccagcgcccg tacggttctt acgtcatgga aaatgtgctg
    24661 ttcaaaatct ccttcccggc ggagttccac tcccagacgg cagttgaagc ggcgatgacg
    24721 ctctatgaac agatgcaggt agcaggcaaa acggcggcgg atatcgaaaa agtgaccatt
    24781 cgcacccacg aagcctgtat tcgcatcatc gacaaaaaag ggccgctcaa taacccggct
    24841 gaccgcgacc actgcattca gtacatggtg gcgatcccgc tgctgttcgg gcgcttaacg
    24901 gcggcagatt acgaggacaa cgtggcgcaa gataaacgca tcgacgccct gcgcgagaag
    24961 atcaattgct ttgaagatcc ggcatttacc gctgactacc acgacccgga aaaacgtgcc
    25021 atcgccaatg ccataaccct tgagttcacc gacggcacgc gatttgaaga agtggtggtg
    25081 gagtacccaa ttggtcatgc tcgccgccgt caggatggca ttccgaagct ggtcgataaa
    25141 ttcaaaatca atctcgcgcg ccagttcccg actcgccaac agcagcgcat tctggacgtt
    25201 tctctcgaca gagctcgcct ggaacagatg ccggtcaatg agtatctcga cctgtacgtc
    25261 atttaagcaa acagcggtaa ggcgtaagtt caacaggaga gcattatgtc ttttagcgaa
    25321 ttttatcagc gttcgattaa cgaaccggag cagttctggg ccgagcaggc ccggcgtatt
    25381 gactggcaga cgccctttac gcaaacgctc gaccacagca acccgccgtt tgcccgttgg
    25441 ttttgtgaag gccgaaccaa cttgtgccac aacgctatcg accgctggct ggagaaacag
    25501 ccagaggcgc tggcgctgat tgccgtctct tcggaaacag aagaagagcg cacctttacc
    25561 tttcgtcagc tgcatgacga agtgaacgcg gtggcgtcaa tgctgcgctc actgggcgtg
    25621 cagcgtggcg atcgggtgct ggtgtatatg ccgatgattg ccgaagcgca tattactctg
    25681 ctggcctgcg cgcgcattgg cgctattcac tcggtggtgt ttgggggatt tgcctcgcac
    25741 agcgtggcgg cgcgaattga tgatgctaaa ccggtgctga ttgtctcggc tgatgccgga
    25801 gcgcgcggtg gcaaaatcat tccctataaa aaattgctcg acgatgcgat aagtcaggcg
    25861 cagcaccagc cacgccatgt tttgctggtg gatcgcgggc tggcgaaaat ggcacgcgtc
    25921 agcgggcggg atgtcgattt cgcgtcgttg cgccatcaac acatcggcgc gcgggtaccg
    25981 gtggcgtggc tggaatccaa cgaaacctcc tgcattctct acacctccgg cacgaccggc
    26041 aaacctaaag gcgtgcagcg tgacgtcggc ggatatgcgg tggcgctggc gacctcgatg
    26101 gacaccattt ttggcggcaa agcgggcggc gtgttcttct gtgcctcgga tatcggctgg
    26161 gtggtggggc attcgtatat cgtctacgcg ccgctgctgg cggggatggc gactatcgtt
    26221 tacgaaggat taccgacctg gccggactgc ggcgtgtggt ggaaaatcgt cgagaaatat
    26281 caggttagcc gcatgttctc agcgccgacc gccattcgcg tgctgaaaaa attccctacc
    26341 gctgaaattc gcaaacacga tctctcgtcg ctggaagtgc tctatctggc tggagaaccg
    26401 ctggacgagc cgaccgccag ttgggtgagc aatacgctgg atgtgccggt catcgacaac
    26461 tactggcaga ccgaatccgg ctggccgatt atggcgattg ctcgcggtct ggacgacagg
    26521 ccgacgcgtc tgggaagccc cggcgtgccg atgtatggct ataacgtgca gttgcttaat
    26581 gaagtcaccg gcgaaccgtg tggcgtcaac gagaaaggga tgctggtggt ggaagggcca
    26641 ttgccgccgg ggtgtattca gaccatttgg ggcgacgacg accgctttgt gaagacgtac
    26701 tggtcgctgt tttcccgtcc ggtgtacgcc acttttgact ggggtatccg cgatgctgac
    26761 ggttatcact ttattctcgg gcgcactgac gatgtgatta acgttgccgg gcatcggctg
    26821 gggacgcgcg agattgaaga gagtatctcc agtcatccgg gcgttgccga agtggcagtg
    26881 gttggggtga aagatgcgct gaaagggcag gtggcggtgg cgtttgtcat tccgaaagag
    26941 agcgacagtc tggaagaccg agacgtggcg cactcgcaag agaaggcgat tatggcgctg
    27001 gtggacagcc agattggcaa ctttggtcgc ccggcgcacg tctggtttgt ctcgcaattg
    27061 ccgaaaacgc gatccggaaa aatgctgcgc cgcacgatcc aggcgatttg cgaaggacgc
    27121 gatcctgggg atctgacgac cattgatgat cctgcgtcgt tggatcagat ccgccaggcg
    27181 atggaagagt aggttattgt cggatgcgtc gcctggcgca tccgacacca tgcgccgatg
    27241 cctgatgcga cgcttaccgc gtcttattat ggctacccac tgtttttaca ccgataattt
    27301 ttcccccacc tttttgcact cattcatata aaaaatatat ttccctacga aaacgattgc
    27361 tttttatctt cagatgaata gaatgcggcg gattttttgg gtttcagaca gcaaaaaggg
    27421 ggaatttcgt gtcgcaagat aacaacttta gccaggggcc agtcccgcag tcggcgcgga
    27481 aaggggtatt ggcattgacg ttcgtcatgc tgggattaac cttcttttcc gccagtatgt
    27541 ggaccggcgg cactctcgga accggtctta gctatcatga tttcttcctc gcagtcctca
    27601 tcggtaatct tctcctcggt atttacactt catttcttgg ttacattggc gcaaaaaccg
    27661 gcctgaccac tcatcttctt gctcgcttct cttttggtgt taaaggctca tggctgcctt
    27721 cactgctgct gggcggcacc caggttggct ggtttggcgt tggcgtggcg atgtttgcca
    27781 ttccggtggg caaggcaacc gggctggata ttaatttgct gattgccatt tccggtttac
    27841 tgatgaccgt caccgtcttc tttggcattt cggcgctgac ggttctttcg gtgattgcgg
    27901 ttccggctat cgcctgcctt ggcggttatt ccgtgtggct ggccgttaac ggcatgggcg
    27961 gcctggacgc attaaaagcg gtcattcccg cacaaccgtt agatttcaat gtcgcgctgg
    28021 cgctggttgt ggggtcattt atcagtgcgg gcacgctcac cgctgacttt gtccggtttg
    28081 gtcgtaatgc caaactggcg gtgctggtgg cgatggtggc ctttttcctc ggcaactcgt
    28141 tgatgtttat tttcggtgca gcgggcgcgg cggcactggg gatggcggat atctctgatg
    28201 tgatgattgc tcagggtctg ctgctgcctg cgattgtggt gctggggctg aatatctgga
    28261 ccaccaacga taacgcgctc tatgcgtcgg gcttaggttt cgccaacatt actggtatgt
    28321 cgagcaaaac cctttcggta atcaacggta ttatcggtac ggtctgcgca ttatggctgt
    28381 ataacaattt tgtcggctgg ctgaccttcc tttcggcagc tattcctccg gtagggggcg
    28441 tgatcatcgc tgactatctg atgaaccgtc gccgctatga gcactttgcg accacgcgta
    28501 tgatgagtgt caattgggtg gcgattctgg cggtcgcctt ggggattgcc gcaggccact
    28561 ggttaccggg tattgttccg gtcaacgcgg tattaggtgg cgcgctgagc tatctgatcc
    28621 ttaacccgat tttgaatcgc aaaacgacgg ctgcaatgac gcatgtggag gctaacagtg
    28681 tcgaataacg ctttacagac aattattaac gcccggttac ccggcaaaga ggggctgtgg
    28741 cagattcatc tgctggacgg aaaaatcagc gccattgatg cgcaatccgg ggtgatgccc
    28801 gtaactgaaa acagcctgga tgccgaacaa ggtttagtta taccgccgtt tgtggagcca
    28861 catattcacc tggacaccac gcaaaccgcc ggacaaccga actggaatca gtctggcacg
    28921 ctgtttgaag gcattgaacg ctgggccgag cgcaaagcgt tattaaccca tgacgatgtg
    28981 aaacaacgcg cctggcaaac gctgaaatgg cagattgcca acggcattca gcatgtgcgt
    29041 acccatgtcg atgtttcgga tgcaacgctg accgcgctga aagcaatgct ggaagtgaag
    29101 caggaagtcg cgccgtggat tgatctgcaa atcgtcgcct tcccgcagga agggattttg
    29161 tcgtatccca acggtgaagc gttgctggaa gaggcgttac gcttaggggc agacgtagtg
    29221 ggggcgattc cgcattttga atttacccgt gaatatggcg tggagtcgct gcataaaacc
    29281 ttcgccctgg cgcaaaaata cgaccgtctc atcgatgttc actgtgatga gattgatgac
    29341 gaacagtcgc gctttgttga aaccgttgct gccctggcgc accgtgaagg catgggcgcg
    29401 cgagtcaccg ccagccacac tacggcaatg cactcctata acggggcgta tacctcgcgc
    29461 ctgttccgct tgctgaaaat gtccggtatt aactttgtcg ccaacccgct ggtcaatatt
    29521 catctgcaag gacgtttcga tacgtatcca aaacgtcgcg gcatcacgcg cgttaaagag
    29581 atgctggagt ccggcattaa cgtctgcttt ggtcacgatg atgtcttcga tccgtggtat
    29641 ccactgggaa cggcgaatat gctgcaagtg ctgcatatgg ggctgcatgt ttgtcagttg
    29701 atgggctacg ggcagattaa cgacggcctg aatttaatca cccaccatag cgccaggacg
    29761 ttgaatttgc aggattacgg cattgccgcc ggaaacagcg ccaacctgat tatcctgcct
    29821 gcagaaaatg ggtttgatgc gctgcgccgt caggttcctg tacgttattc ggtacgtggc
    29881 ggtaaggtga ttgccagcac acaaccggca caaaccaccg tatatctgga tcagccagaa
    29941 gccatcgatt acaaacggta atttcctctg tgttgaagag ccactgacct gtgtcagtgg
    30001 ctaccgtgat tcattcccgc caacaaccgc gcattcctcc aacgccatgt gcaaaaatgc
    30061 cttcgcggcg gctgtctgcc agctattttt ccgccgcaat aaaaccgccg ttctctccag
    30121 taacggtggg gcaagggata tagctttaag cccgtcatgt tgtgtggcaa tcgctgctgg
    30181 caacaatgtg gaaagggaag tgcggcgaat cagctccaga accgcgctaa ttgagttcgc
    30241 ctcaatgacc acctgtggat gtagccccgc tttctcgcag tagtggtcaa tttgctctct
    30301 ggtggcaaat tccgcgctga gcaggaccag tttttcatca tgcaagcgac tcaacgccac
    30361 ctgttcacag gcagccagcg gatgatgttg cgccacgact aacgctaaac tttctgtcag
    30421 taaaggaatt gcctccagct ccggcgaatg cacaggcgcg aaggcaatcc caacgtccaa
    30481 ctcgtcgcga caaagcagat cttcgatttt ctcctgcgac atttcctgta gctggagcgt
    30541 gatgccgggg tagcgcgcat agaaatccgc cattaaaggg ccgataaagt agcttgtaaa
    30601 ggtgggagtg acggcgatac gcagcgatcc tcgcgtcaga tcggcaacat catgaatcgc
    30661 ccgtttaccc gcccccagtt cctgtaacgc ccggctggcg tactgtcgcc agacttctcc
    30721 tgcatcagtg agacgaatcg ttcgcccgct acggtcaaac agcggcacgc ctaaactctc
    30781 ctctaactgg cgaatctgct gggaaagcgc aggctgggag acgtgcaacg cactggcggc
    30841 acgggtaaag ctgccatgtt cagccacggc aagaaaataa ttgatatgtc gagagagcat
    30901 tcgccaacct ataagtaaat ccaatagaac tcatcataaa tgagactttt accttatgac
    30961 aatcggcgag tagtctgcct ctcattccag agacagacag aggttaacgg tgaaagagat
    31021 tattgatgga ttccttaaat tccagcgcga ggcatttccg aagcgggaag ccttgtttaa
    31081 acagctggcg acacagcaaa gcccgcgcac actttttatc tcctgctccg acagccgtct
    31141 ggtccctgag ctggtgacgc aacgtgagcc tggcgatctg ttcgttattc gcaacgcggg
    31201 caatatcgtc ccttcctacg ggccggaacc cggtggcgtt tctgcttcgg tggagtacgc
    31261 cgtcgctgcg cttcgggtat ctgacattgt gatttgcggt cattccaact gtggcgcaat
    31321 gaccgctatt gccagctgcc agtgcatgga ccatatgcct gccgtctctc actggctgcg
    31381 ttatgccgat tcagcccgcg tcgttaatga ggcgcgcccg catcccgatt taccgtcaaa
    31441 agctgcggcg atggtacgtg aaaacgtcat tgctcagttg gctaatttgc aaactcatcc
    31501 atcggtgcgc ctggcgctcg aagaggggcg gatcgccctg cacggctggg tctacgacat
    31561 tgaaagcggc agcatcgcag cttttgacgg cgcaacccgc cagtttgtgc cactggccgc
    31621 taatcctcgc gtttgtgcca taccgctacg ccaaccgacc gcagcgtaac cttattttta
    31681 aaccatcagg agatccacca tgattcagtc gcaaattaac cgcaatattc gtcttgatct
    31741 tgccgatgcc attttgctca gcaaagctaa aaaagatctc tcatttgccg agattgccga
    31801 cggcaccggt ctggcagaag cctttgtaac cgcggctttg ctggggcagc aggcgcttcc
    31861 tgccgacgcc gcccgcctgg tcggagcgaa gctggatctc gacgaagacg ccattctact
    31921 gttgcagatg attccactgc gtggctgcat tgatgaacgt attccaaccg acccaacgat
    31981 gtatcgtttc tatgaaatgt tgcaggtgta cggcacaacc ctgaaagcgt tggttcatga
    32041 gaagtttggc gatggcatta tcagcgcgat taacttcaaa ctcgacgtta agaaagtggc
    32101 ggacccggaa ggtggcgaac gtgcggtcat caccttagat ggtaaatatc tgccgaccaa
    32161 accgttctga cagccatgcg caaccatcaa aagacgttca cgatgctgct ggttctggtg
    32221 ctgattggtc ttaatatgcg accactgctc acctccgtcg ggccactgct accgcaattg
    32281 cgccaggcaa gcggaatgag ctttagcgtg gctgccctgt tgaccgctct gccggtggtt
    32341 accatgggcg ggctggcgct ggccggaagc tggcttcatc agcatgtcag cgaacgtcgc
    32401 agcgtcgcca tcagtttgtt actgattgcc gtcggtgcat tgatgcgtga gctttacccc
    32461 caaagtgcgc tgctgcttag cagcgcactg cttggtggag tggggatcgg catcattcag
    32521 gcagtgatgc cttcggtgat taaacggcgg tttcagcagc gcacgccact ggtgatgggg
    32581 ctgtggtccg cggctctgat gggcggcggt ggacttggtg ccgccatgac gccatggtta
    32641 gttcaacata gcgaaacctg gtatcaaaca ctcgcctggt gggcgctgcc tgccgttgtt
    32701 gcgctctttg cctggtggtg gcaaagcgcc cgcgaggtcg cctcttctca caagacaaca
    32761 accactccgg ttcgcgtggt atttactccc cgcgcgtgga cgctgggtgt ttacttcggt
    32821 ctgattaacg gcggttacgc cagcctgatt gcctggttac ccgctttcta tattgagatt
    32881 ggtgccagcg cgcagtacag cggttccttg ctggcattga tgacgcttgg gcaagcagca
    32941 ggggcattgc tgatgcctgc tatggctcgc catcaggatc ggcgcaaact tttgatgctg
    33001 gcgctggcgt tacaactggt ggggttctgc ggctttatct ggctgccgct gcaattgccg
    33061 gtattgtggg caatggtgtg tgggttaggt ctgggcggcg catttccgct ctgtttgctg
    33121 ctggcgctcg atcactctgc gcaacccgct attgctggca aactggtggc gtttatgcag
    33181 gggatcggtt ttatcatcgc cgggcttgcc ccgtggtttt ctggtgtgct gcgtagtatc
    33241 agtggcaatt acttgatgga ctgggcgttt catgcgctgt gcgtcgttgg gctgatgatc
    33301 ataaccctgc gttttgcacc agcacgtttt ccgcagctgt gggtcaaaga ggcatgatgc
    33361 gacgcttgtt tcgccagata atggaagagt gaattattgt cggatgtgcc caatggcgca
    33421 tccgacattt atttcagtgc taatttaaac tgaaggttca a
//
LOCUS       DACBVI010000040        32278 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.040, whole genome shotgun sequence.
ACCESSION   DACBVI010000040 DACBVI010000000
VERSION     DACBVI010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.040"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(186..3335)
                     /locus_tag="IU325_09040"
     CDS             complement(186..3335)
                     /locus_tag="IU325_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HAP5809171.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTNYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3358..4551)
                     /gene="acrA"
                     /locus_tag="IU325_09045"
     CDS             complement(3358..4551)
                     /gene="acrA"
                     /locus_tag="IU325_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HAP5809172.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     AAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4693..5340
                     /gene="acrR"
                     /locus_tag="IU325_09050"
     CDS             4693..5340
                     /gene="acrR"
                     /locus_tag="IU325_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HAP5809173.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5468..8830
                     /locus_tag="IU325_09055"
     CDS             5468..8830
                     /locus_tag="IU325_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lymphostatin Efa1/LifA"
                     /protein_id="HAP5809174.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8869..9033)
                     /gene="rsmS"
                     /locus_tag="IU325_09060"
     CDS             complement(8869..9033)
                     /gene="rsmS"
                     /locus_tag="IU325_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HAP5809175.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LQSDEASQSE"
     gene            complement(9047..9574)
                     /gene="priC"
                     /locus_tag="IU325_09065"
     CDS             complement(9047..9574)
                     /gene="priC"
                     /locus_tag="IU325_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HAP5809176.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNMAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9644..10021
                     /locus_tag="IU325_09070"
     CDS             9644..10021
                     /locus_tag="IU325_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HAP5809177.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10174..10725
                     /locus_tag="IU325_09075"
     CDS             10174..10725
                     /locus_tag="IU325_09075"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP5809178.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10854..12785
                     /locus_tag="IU325_09080"
     CDS             10854..12785
                     /locus_tag="IU325_09080"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP5809179.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    12118..12182
                     /locus_tag="IU325_09080"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12838..13167
                     /locus_tag="IU325_09085"
     CDS             12838..13167
                     /locus_tag="IU325_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP5809180.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13167..13772
                     /gene="recR"
                     /locus_tag="IU325_09090"
     CDS             13167..13772
                     /gene="recR"
                     /locus_tag="IU325_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP5809181.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13882..15756
                     /gene="htpG"
                     /locus_tag="IU325_09095"
     CDS             13882..15756
                     /gene="htpG"
                     /locus_tag="IU325_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HAP5809182.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            15937..16581
                     /locus_tag="IU325_09100"
     CDS             15937..16581
                     /locus_tag="IU325_09100"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP5809183.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            16713..17675
                     /locus_tag="IU325_09105"
     CDS             16713..17675
                     /locus_tag="IU325_09105"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HAP5809184.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQYSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17672..18631)
                     /locus_tag="IU325_09110"
     CDS             complement(17672..18631)
                     /locus_tag="IU325_09110"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HAP5809185.1"
                     /translation="MKLENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18783..20087
                     /locus_tag="IU325_09115"
     CDS             18783..20087
                     /locus_tag="IU325_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965517.1"
                     /note="catalyzes the formation of inosine/guanosine
                     monophosphate from inosine or guanosine and ATP; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HAP5809186.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20217..21893)
                     /locus_tag="IU325_09120"
     CDS             complement(20217..21893)
                     /locus_tag="IU325_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HAP5809187.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22131..23351)
                     /locus_tag="IU325_09125"
     CDS             complement(22131..23351)
                     /locus_tag="IU325_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5809188.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKE"
     gene            23569..25221
                     /gene="ushA"
                     /locus_tag="IU325_09130"
     CDS             23569..25221
                     /gene="ushA"
                     /locus_tag="IU325_09130"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308560.1"
                     /note="catalyzes the degradation of periplasmic
                     UDP-glucose to uridine, glucose-1-phosphate and inorganic
                     phosphate; specific for uridine nucleotides; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAP5809189.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKLGNPENFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQAPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLDVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKINGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25258..25737)
                     /gene="ybaK"
                     /locus_tag="IU325_09135"
     CDS             complement(25258..25737)
                     /gene="ybaK"
                     /locus_tag="IU325_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HAP5809190.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(25941..26735)
                     /locus_tag="IU325_09140"
     CDS             complement(25941..26735)
                     /locus_tag="IU325_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809191.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISTSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDNGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLCAMPPGRYVVAVGALHLYGEGNLSQMLR"
     gene            complement(27164..29668)
                     /gene="copA"
                     /locus_tag="IU325_09145"
     CDS             complement(27164..29668)
                     /gene="copA"
                     /locus_tag="IU325_09145"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HAP5809192.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSVTEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMLGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     NVPLADVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHAVLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            29931..30863
                     /gene="glsA"
                     /locus_tag="IU325_09150"
     CDS             29931..30863
                     /gene="glsA"
                     /locus_tag="IU325_09150"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP5809193.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKVGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            30866..32158
                     /locus_tag="IU325_09155"
     CDS             30866..32158
                     /locus_tag="IU325_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP5809194.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHALLPILIVGTLVMLLVI
                     IGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
BASE COUNT         7777 a         8542 c         8520 g         7439 t
ORIGIN      
        1 attattaaat ttattagcgc tacttaatat aaattaacta acaatcagta ttttttatga
       61 attttatgcg aggttaatac tggttttcgt atgagatcct gagttggtgg ttcaatgact
      121 ccttaatgtt cgtcggttat gcataaaaaa ggccgcttgc gcggcctttg tgattacacg
      181 ttgtatcaat gatgatcgac agtatggctg tgctcgatat cttcattctt gcggctaaag
      241 cggcggcgaa ccaccacaaa gaataccgga acgaagaaga ttgccagtac cgttgcggtc
      301 accatcccgc ccattacacc ggtacctact gcgttctgcg cgccggaacc agcaccagta
      361 ctgataacca gcggcataac gccgaggata aacgccagcg aggtcatcag gatcggacgt
      421 aaacgcatcc gcaccgcatc aagcgtcgct tcaatcagac ctttaccttc tttatccatc
      481 aagtctttgg cgaattcgac gataagtatc gcgttcttcg ccgacaaccc aatggttgtg
      541 agcaggccta cctggaagta aacgtcattg gtcaggccac ggaaggtggc agccagcaac
      601 gcaccgataa cccccagcgg aacgaccagc ataacggaga acggaatcga ccagctctcg
      661 tacagcgccg ccagacacag gaacacgaca atcaacgaaa tcgcgtacag tgaaggtgcc
      721 tggttgccgg agagacgttc ctgataggac atccccgtcc agtcatagcc aacaccggta
      781 ggcagtttgc tcgccagttg ttccatcagc tccattgctt caccggtact tttacccggt
      841 gccgcctggc ctaagatttc catggatggc aggccgttgt aacgttccag acgcggcgaa
      901 ccgtactccc aacgagaaga ggagaacgcc gagaacggca ccatctgacc atcagcagca
      961 cgaacatacc agtcgccgat atcatccggc agcatacggt atttcgcttc tgacatgacg
     1021 taaactttct tcacacgacc gcggtcgata aagtcgttca catagctacc gccccatgca
     1081 gcgcccagag tggtgttaat gtcgttgata gaaacaccca gcgcctgcgc tttttcctgg
     1141 tcgatatcaa tcttaaactg cggggtatct tccagaccgt ttggacgtac gctggtcaac
     1201 atatcagggt gcttcgctgc ttctgcaagc agctggttac gcgcctgagt cagtttttcg
     1261 tgaccaaggc cagcctggtc aatcagctca aagtcaaagc cggttgcagt acccagttcc
     1321 acgattgcgg gcaggttaaa ggcgaaaacc atcgcatctt tgatttgcga gaaagcgcgt
     1381 gttgcacgca tggtaatcgc ttcaactttg ttttcttcac ccggacgatc ggcccagtcc
     1441 ttcaaggaaa cgaacgcaat accggtattc tgaccacgtc ccgcaaagcc gaagccgtta
     1501 acggcgaaca ccgactcaac gttgttcttt tctttggtca gatagtaatt cgttacctca
     1561 ttgagcactt tctgcgtacg ttcctgcgtt gcacctgctg gcagctgaac catggtcata
     1621 aatacgccct ggtcttcatc tggcaagaag gagcttggca gacgcacgaa cagataggcc
     1681 atgccgacca cgatgatcag atacagcacc aggtaacgcc ctgtactgcg cagaataccg
     1741 cctacgctgt cggtgtagtg gtgcgtgctc ttctcgaaca tgcggttaaa ccagccgaag
     1801 aagccttttt taccttcccc gtgatcgcct ttggcaatcg gtttcagcat ggtggcacaa
     1861 agagccggag tcaggatcaa cgccaccagt actgacagcg ccattgctga aacaatggta
     1921 atagagaact ggcgatagat agcaccagta gaaccgccaa agaaggccat cggaacgaat
     1981 accgccgaca gtaccatcgc gataccgacc agagcgccct gaatctgccc catcgactta
     2041 cgggtagctt cttttggcgg caaaccttct tccgccatca cacgctcaac gttttctacc
     2101 acaacgatgg cgtcatccac caacaggccg atggcgagca ccatcccgaa cattgttagc
     2161 gtgtttatcg agaagccaaa ggcggcaagg acggcaaagg tccccagcaa taccaccggt
     2221 acggcaatgg tcggaatcaa cgtcgcgcgg aagttctgca agaacagata cataaccagg
     2281 aacacgagga tgatcgcttc gaccagcgtt ttaaccactt cgtgaataga gattttcacg
     2341 aacggcgtgg tgtcgtacgg gtaaacaatt ttcagacctg acgggaagaa cggttccatc
     2401 ttcgccagtt cagcacggat tgccgcagcg gtatccagcg cgtttgcacc ggtcgccagc
     2461 ttgatcccca gaccggaagc cggttggccg ttaaactctg cgatgatgtc gtagttctca
     2521 ccacccagct caattttcgc cacatcacgc agcagcacgc gggaaccatc ctgattcact
     2581 ttcagcagga ttttgccaaa ctcttcagta gaggtcagac gcgtctgagc aataatagag
     2641 gcgttaagct gttggccttt caccggcggc gtaccaccga gctgacccgc cgcaacctgg
     2701 gcgttctgcg ctttgatggc ggtaatgaca tcaaccggtg ttagttggaa tttgttcagt
     2761 tcattcgggt tcatccagat acgcatcgcg tactgtgaac cgaacaactg aacgtcaccc
     2821 acgcccgacg tacggctgat ggcatctttc atattcgccg ccacgtagtc ggagatatcc
     2881 tcctgcgtca tggtgccatc ggtgttgata acgccgacaa ccatcaggaa gctgctggat
     2941 gatttctcaa cgctcacccc ttgctgctgt acttcttgcg gcagcaacgg catcgccagc
     3001 tgcagtttgt tctgcacctg aacctgcgcg atatccgcat cagtaccaga ctcaaaggtc
     3061 agggtgatct gcacggtacc cgtggagtca ctgttagagg acatgtacat caggttatcg
     3121 ataccgttca tattctgttc gataacctgt gtcaccgtgt cctgcactgt tttcgcatca
     3181 gcgccggggt aggaggcgga gatcgttact gccggcggag caatcgtagg atattgcgcc
     3241 accggcagtt tgaggatcgc cagcccccct gccaacatga tgataatggc gatcacccac
     3301 gcaaaaatcg ggcgatcgat aaagaaatta ggcatgtctt aacggctcct gtttaagtta
     3361 agacttggac tgttcaggct gagcaccgct tgcggcttgc tggttattat cagcggtaac
     3421 ttcttgtgct tttacctgga caccaggacg cactttctgc agcccactta ttactacgcg
     3481 atcgcctgct ttcagacctt ctgtcaccag ccacttatcg ccaatagcct ggcttgcaac
     3541 gatcggacgg gtttccactt tgtcatccgc gccaaccacc agtacggtgg catcgccacg
     3601 cggcgtacgg gttacgccct gttgcgggac taaaatagcg tttggattaa gcccttcttc
     3661 cagacgtgcg cgcacgaaca tacccggcag cagagtgtga tccgggttcg ggaagatagc
     3721 gcgtagggtg atagacccgg tggtctgatc aacggtaacg tcagagaatt ccagcgtacc
     3781 gtcctgcggg aacttaatgc cgtcactggt gatcagcgac actttggctt tgccgttctc
     3841 ttgtttcagc gtgccattcg ccagttcctg tttcaggcgc aggaagtcgt tgctggactg
     3901 ggtcacatca acgtagatcg gatcaagttg ctgcacggtt gccagcgcag tcgcctgacc
     3961 gttctgtacc aatgcgcctt ccgtcacgtt cgacttacca atgcgaccgc taatcggaga
     4021 ggtaactttg gtgtaagcca gattgatccg cgcagtttca acggcagctt tcgccgcagt
     4081 taccgcagca ttcgcctgtt gcgcatcagc cagagcctga tcgtactctt gcttactgat
     4141 gtactgagta ccgagcaatt tctgataacg attcaccgtc aattgcgcga tattggctgc
     4201 agcctgggct ttcgccagat cgcctttcgc gctgtcatat gccgcctgat aggtcgcagg
     4261 atcaatctga tagagagaga cacctgcttc gatgtcgcta ccttctttga aattacgctt
     4321 caggataatc ccgctaactt gaggacgaac ttctgcgatc cggtaggcac tggtgcgacc
     4381 cggaagctcg gttgtgatct gcagaggttc agttttgact gttactacgc caacggcggg
     4441 catctgctgg ccaccttgtt gggcctgttt gtcgtcacat cctgttaggg ctaagctgcc
     4501 tgagagcatc agaacgaccg ccagaggcgt aaaccctctg tttttgttca tatgtaaacc
     4561 tcgagtgtcc gatttcaaat tggtcaatgg tcaaaagtta ataaacccat tgctgcgttt
     4621 atattatcgt cgtgctatgg tacatacatt cacaaatgta tgtaaatcta acgcctgtaa
     4681 attcacgaac atatggcacg aaaaaccaaa caagaagcgc aagaaacgcg ccaacacatc
     4741 ctcgatgtgg ctctacgtct tttctcacag cagggggtat catccacctc gctgggtgag
     4801 attgcaaaag cagctggcgt tacgcgcggt gcaatctact ggcattttaa agacaagtcg
     4861 gatttgttca gtgagatctg ggaactgtca gaatccaata ttggtgaact agagcttgag
     4921 tatcaggcaa aattccctgg cgatccactc tcagtattaa gagagatatt aattcatgtt
     4981 cttgaatcca cggtgacaga agaacggcgt cgattattga tggagattat attccacaaa
     5041 tgcgaatttg tcggagaaat ggctgttgtg caacaggcac aacgtaatct ctgtctggaa
     5101 agttatgacc gtatagaaca aacgttaaaa cattgtattg aagcgaaaat gttgcctgcg
     5161 gatttaatga cgcgtcgggc agcaattatt atgcgcggct atatttccgg cctgatggaa
     5221 aactggctct ttgccccgca atcttttgat cttaaaaaag aagcccgcga ttacgtcgcc
     5281 atcttactgg agatgtatct cctgtgcccc acgcttcgta atcctgccac taacgaataa
     5341 ccctgaatct gactccagga tttttcctgg acattttcgt cgttgctatt ctggttcact
     5401 gcgtcgtgat attcttgcgg tttgactttt tcaggtcgtt cttcaggttc agaaaccttc
     5461 attcatcatg actatgttcc agtattacaa acgatcacgg cattttgttt tttcagcatt
     5521 tattgctttt gtttttgtct tgttatgcca gaacacggcg tttgcgcggg cgtcatcgaa
     5581 tggtgatctg ccgacaaaag cggacctgca ggcacaactt gactcactga ataaacaaaa
     5641 agatctttct gctcaggaca aactggtgca gcaggatctg acagatacat tagccaccct
     5701 cgataaaatc gatcgcgtaa aagaagagac agttcagcta cggcaaaaag tcgctgaagc
     5761 gccggaaaaa atgcgccagg cgaccgcggc gttaactgca cttagcgatg tcgataatga
     5821 cgaagaaacg cgcaaaattc tgagtacgct ttcgttgcgc cagctggaaa ctcgcgttgc
     5881 ccaggcgctg gacgatttgc agaacgcaca aaacgatctg gcgtcttata acagccagtt
     5941 ggtttcgtta cagacgcagc ccgaacgcgt gcaaaatgcg atgtataacg cttcgcagca
     6001 gctgcaacaa attcgcagtc gtctggatgg gactgatgtc ggcgagactg ccttacgtcc
     6061 cagccagaaa gtgttaatgc aggctcagca ggctttgctg aatgccgaga ttgaccagca
     6121 gcgtaaaagc ctggaaggga acacagtctt acaggatacc ttgcaaaagc aacgggatta
     6181 cgtgacggcg aacagcgccc gtctggagca ccagttgcag ctgttgcaag aagcagtaaa
     6241 cagcaagcgc ctgactttaa ccgaaaaaac ggcgcaggaa gccgtctccc cggatgaagc
     6301 cgcgcgtatt caggctaatc cgctggtgaa gcaggaactg gaaattaacc agcagttaag
     6361 tcagcgtctg attaccgcga ctgaaaacgg taatcagttg atgcagcaaa acattaaagt
     6421 caaaaactgg ctggagcggg cgctgcaatc ggaacgcaat attaaagagc agattgccgt
     6481 cctgaagggc agcctgctgt tgtctcgtat cctttaccag caacaacaaa cgctgccctc
     6541 ggcggatgag ctggaaaaca tgaccaaccg catcgcggat ttgcgtctcg aacagtttga
     6601 agttaaccag cagcgtgatg cactcttcca gagcgatgcg ttcgtcaaca aactggaaga
     6661 aggtcacacc aacgaagtca acagcgaagt tcacgatgcg ttattgcaag tagttgatat
     6721 gcgtcgcgaa ttgctggatc agcttaacaa acagttgggt aaccagctga tgatggccat
     6781 taacctgcaa atcaaccagc agcagttaat gagtgtgtcg aaaaacctga aatccatcct
     6841 gactcagcaa atcttttggg tgaacagtaa ccgtccaatg gactgggact ggatcaaagc
     6901 gttcccgcaa agcctgaaag atgaatttaa gtcgatgaaa atcacagtga actgggaaaa
     6961 agcatggcct gccgttttta tcgctttcct cgccggtttg ccgctgctgt tgattgccgg
     7021 gctgatccac tggcgtctgg gctggctgaa agcgtatcaa cagaaactgg cttccgctgt
     7081 gggttccctg cgtaacgaca gccagctcaa cacaccaaaa gcgatcctta tcgacctgat
     7141 ccgtgcgctg ccggtgtgcc tgattattct cgcggttggc ctgattctgt tgaccatgca
     7201 actcaacatc agcgaactgc tatggtcgtt cagtaaaaaa ctggcgattt tctggctggt
     7261 gtttggcctg tgctggaagg tactggagaa aaacggcgtt gccgtgcgtc actttggtat
     7321 gccggaacag cagaccagcc actggcgtcg gcaaattgtc cgcatcagtc tcgcattgct
     7381 gcctatccat ttctggtctg tggtggcaga actttccccg ctgcatctga tggatgatgt
     7441 gctggggcag gcgatgattt tcttcaacct gctgctgatt gccttcctgg tatggccgat
     7501 gtgccgcgaa agctggcgtg ataaagagtc gcacaccatg cgactggtca ccattaccgt
     7561 gctgtcgatt atcccgattg cgttgatggt gctgaccgcc acgggttatt tctacaccac
     7621 gctgcgcctc tccgggcgtt ggattgaaac cgtttatttg gtgatcatct ggaacctgct
     7681 gtaccagacg gtattgcgcg gcttaagcgt agcggcgcgg cgtatcgcct ggcgtcgtgc
     7741 gctggcgcgt cggcagaatc tggtgaaaga gggcgcggaa ggtgctgaac cgccggaaga
     7801 acccaccatt gcactggagc aggttaacca gcagacgctg cgtattacca tgttgctgat
     7861 gtttgcgctg ttcggtgtca tgttctgggc aatttggtcc gatttgatca ccgtgttcag
     7921 ctatctggac agcatcacgc tctggcatta caacggcact gaagctggcg ctgcggtggt
     7981 gaaaaacgtc accatgggca gtctgttgtt tgcgattatc gcctcaatgg tagcctgggc
     8041 gttgattcgc aacctgcctg gtctgttgga agtgttggtg ctctcgcgcc tgaacatgcg
     8101 gcagggcgcg tcgtatgcca ttactaccat ccttaactac atcattattg ccgttggtgc
     8161 gatgacggtg ttcggatcgc tgggcgtctc gtgggataaa ctccagtggc tggcggcagc
     8221 attatccgta ggtcttggtt ttggtttaca agagatcttc ggtaacttcg tctccggttt
     8281 gatcattctg ttcgaacgtc cggtgcgcat tggcgatacg gtaaccattg gtagcttctc
     8341 ggggacggta agtaagatcc gtattcgtgc gacaacgatt accgatttcg atcgcaaaga
     8401 agtgatcatc ccgaacaaag cgtttgttac cgagcgtctg atcaactggt cgttgactga
     8461 cactactacg cgtctggtga tccgtctcgg cgtggcctat ggctccgatc tggaaaaagt
     8521 gcgtaaagtg ttactgaagg cggcgactga gcacccaagg gtgatgcacg aaccaatgcc
     8581 ggaagtcttc tttacggcgt ttggtgccag cacgttggat catgagctgc gtctgtatgt
     8641 gcgtgaactg cgtgaccgta gccgtactgt cgatgagctg aaccgtacta tcgatcagct
     8701 gtgccgtgaa aacgatatca acattgcctt taaccagctt gaagtgcatc tgcacaacga
     8761 gaagggcgat gaggtgacgg aagtcaaacg cgactacaaa ggcgacgatc cgacaccagc
     8821 ggtagggtaa ggacaataaa gggcgacagt gatgccgccc tttatttttt actcgctttg
     8881 cgaagcctca tcgctctgta acttcttttc ataatcacgc ttcacaatat ccagcgcgcg
     8941 cagcacggtg cgggctggga tctgattttc ttccagcagc acaatcaaat cgacagccag
     9001 tttgacatcg tcaggggcat tttccagtga catactctct ccattgctag cgggttaagc
     9061 gcgctagcct gttttcgatt ttttccagcg catggcggca gcgtgccagg cgcgcttcat
     9121 aggcttccac ttcacgatgc agcgtttgct gttccacgag atcggtcacc cgcgccaggc
     9181 gggctcgacg ttcggctacc atctcacgta gccgccgctc aaaatcctga tgctgaatac
     9241 gtttacgctg ccagcgggca attttcggtg gcgcactgtc ccactcgcgt agcgaccagg
     9301 ctgtggcttc acgcgctata gcctccagtt gcgccgccag atgttctgcc agccaggcca
     9361 cttgcggcag ctgttgctgc tcaaccgcat gacgaagcgc agccatgtta tcgcccgcct
     9421 catcgagaca agcctgcagt gttgtcgcac gggtctgaaa aaggtgcctg tcaaagcggg
     9481 cacttagcgt ggcaaattgc gctaccgggg cacaacgctg gcgcagcgtg gcgagctgtc
     9541 cttccagttt ttccagcagc agggcggttt tcatgataag acctcaatga aaatgataat
     9601 tgttatgcta aagtagccac tctgtaagct gacaacactc aatatgcaac gaatcatttt
     9661 aatcatcatt ggctggctgg cggtagtgct gggtacgctg ggcgtggtat taccggtatt
     9721 accgacgacg ccgtttatcc tgctggcggc ctggtgcttt gcccgttctt ccccgcgctt
     9781 tcacgcctgg ttgctgtacc gctcatggtt tggcagctat ctacgtttct ggcagaaaca
     9841 tcatgcgatg ccgcgcggcg tcaaaccgcg ggcgattttg cttattttgc tcacgtttgc
     9901 aatttctctg tggttcgtcc agatgccatg ggtgcgcatc atgttgctgg taattctcgc
     9961 ctgtttgctt ttttatatgt ggcgaattcc ggtgattgat gaaaagcaag aaaagcactg
    10021 aagcacaaca atcgcagttg caattattgc gtacagccag tacattctgg cgttttcgag
    10081 cacaggcgca ggcggtcaaa ggttaaacaa ctgttacttt tgatacgttt aaaacgcgcc
    10141 gtgagtacca ccgtaacaag caggcataca cttatgaccg cgactgcaca gcagcttgag
    10201 tatctcaaaa atagcatcaa aagcattcag gactacccaa aacccggcat tcttttccgt
    10261 gatgtcacca gcttactgga agacccgaaa gcttacgctc tcagcatcga cttgctggtt
    10321 gagcgttaca aaaatgcggg cattaccaaa gttgtcggca ccgaagcgcg tggcttcttg
    10381 tttggcgctc cggtagctct gggtctgggc gttggctttg taccggtccg taaaccgggc
    10441 aaactgccgc gtgaaaccat cagtgaaact tacgatctgg aatacggcac cgatcagctg
    10501 gaaatccacg ttgatgccat caaaccgggc gacaaagttc tggtggtgga cgatctgctg
    10561 gcaaccggcg gcactatcga agcgaccgtt aaactgatcc gtcgtctggg tggtgaagtg
    10621 gctgatgctg cgttcattat caatctgttc gatctcggcg gcgaacagcg tctcgaaaaa
    10681 cagggcatta ccagctacag ccttgttccg ttcccgggcc attaattatc gccagtcttg
    10741 tgctgcccac gctacggaca gcacaagatg tgcattcagc ctcgccgttc tgacggggct
    10801 gtgttagcat taccccttcg tgaatccacc ttccagcgtt tcagagcctg ccaatgagtt
    10861 atcaggtctt agcccgaaaa tggcgcccac aaacctttgc tgacgtcgtc ggccaggaac
    10921 atgtgctgac cgcactggcg aacggcttgt cgttagggcg tattcatcat gcttatcttt
    10981 tttccggcac ccgtggcgtc ggaaaaacct ctatcgcccg actgctggcg aaggggctaa
    11041 actgcgaaac cggcattacc gcgacgccgt gcggcgtgtg cgataactgt cgtgaaatcg
    11101 agcaggggcg ctttgtcgat ctgattgaaa tcgatgccgc ctcgcgcact aaagttgaag
    11161 acactcgcga cctgctggat aacgtccagt acgctccggc gcgtggtcgt ttcaaagttt
    11221 atctgatcga cgaagtgcat atgctgtcgc gccacagctt taacgcactg ttaaaaaccc
    11281 ttgaagagcc gccagagcac gttaagtttc tgctggcgac gaccgatccg cagaaattgc
    11341 cggtgacgat tttgtcacgt tgtctgcaat ttcatctcaa ggcgctggat gtcgagcaaa
    11401 ttcgccatca gcttgagcac atcctcaacg aagaacatat cgctcacgag ccgcgggcgc
    11461 tgcaattgct ggcacgcgcc gctgaaggca gcctgcgaga tgccttaagt ctgaccgacc
    11521 aggcgattgc cagcggtgac ggccaggttt caactcaggc ggtcagtgcg atgctgggta
    11581 cgcttgacga cgatcaggcg ctgtcgctgg ttgaagcgat ggtcgaggcc aacggcgagc
    11641 gcgtaatggc actgattaat gaagccgctg cccgtggtat cgagtgggaa gcgttgctgg
    11701 tggaaatgct cggcctgttg catcgtattg cgatggtaca actttcgcct gctgcacttg
    11761 gcaacgacat ggccgctatc gagctgcgga tgcgtgaact ggcgcgcacc ataccgccga
    11821 cggatattca gctttactat cagacgctgt tgattggtcg caaagaatta ccgtatgcgc
    11881 cagaccgccg tatgggcgtt gagatgacgc tgttgcgcgc gctggcgttc catccgcgta
    11941 tgccgctgcc tgagccagaa gtgccacgcc agtcctttgc gcccgtcgcg ccaacggcag
    12001 taatgacgcc aacccaggtg ccgccgcaac cgcaatcagc gccgcagcag gcaccgactg
    12061 taccgctccc ggaaaccacc agccaggtgc tggcggcgcg ccagcagttg cagcgcgtgc
    12121 agggagcaac caaagcaaaa aagagtgaac cggcagccgc tacccgcgcg cggccggtga
    12181 ataacgctgc gctggaaaga ctggcttcgg tcaccgatcg cgttcaggcg cgtccggtgc
    12241 catcggcgct ggaaaaagcg ccagccaaaa aagaagcgta tcgctggaag gccaccactc
    12301 cagtgatgca gcaaaaagaa gtggtcgcta cgccgaaggc gctgaaaaaa gcgctggaac
    12361 atgaaaaaac gccggaactg gcggcgaagc tggcggcaga agccattgag cgcgacccgt
    12421 gggcggctca ggtgagtcaa ctttcgctac caaaactggt cgaacaggtg gcgttaaatg
    12481 cctggaaaga ggagagcgac aacgcagtat gtctgcattt gcgctcctct cagcggcatt
    12541 tgaacaaccg cggcgcacag caaaaactgg ctgaagcgtt gagcatgtta aaaggttcaa
    12601 cggttgaact gactatcgtt gaagatgata atcccgcggt gcgtacgccg ctggaatggc
    12661 gtcaggcgat atacgaagaa aaacttgcgc aggcgcgcga gtccattatt gcggataata
    12721 atattcagac cctgcgtcgg ttcttcgatg cggagctgga tgaagaaagt atccgcccca
    12781 tttgatcgta agcacagctt acgttcgtca tccttaacgt gattgagaga gaaacctatg
    12841 tttggtaaag gcggtctggg taacctgatg aagcaagccc agcagatgca agaaaaaatg
    12901 cagaaaatgc aggaagagat cgcgcagctg gaagtcaccg gcgaatctgg cgcaggtctg
    12961 gtaaaagtga ccattaacgg tgcacacaac tgccgtcgcg tagagatcga cccgagcctg
    13021 ctggaagacg acaaagagat gctggaagac ttggtggctg cagcattcaa cgacgcagca
    13081 cgtcgtattg aagaaacgca gaaagaaaaa atggcctctg tatcctccgg aatgcagctg
    13141 ccgcctggct ttaagatgcc gttctgatgc aaaccagccc gctgttaaca cagcttatgg
    13201 aagcactgcg ctgtctgccg ggcgttggcc cgaagtcggc gcagcgtatg gcgttcacgc
    13261 tgcttcagcg cgatcgtagc ggcgggatgc gtctggcgca ggcgctcacc cgggcgatgt
    13321 cggaaatcgg ccactgcgcc gattgccgca ccttcaccga acaggaagtc tgtaacatct
    13381 gttcgaatcc gcgtcgtcag gaaaacggtc aaatctgcgt ggtggagagt ccggcggaca
    13441 tctacgccat tgagcagacg gggcagtttt caggccgtta ttttgtgttg atggggcacc
    13501 tgtcaccgct ggacggcatc ggtccggatg atatcggcct tgatcgtctg gaacagcgtc
    13561 tggcagagga aaaaatcact gaagtgatcc tcgccaccaa ccccactgtt gaaggtgaag
    13621 ctaccgctaa ctacattgcc gagctttgcg cgcaatatga cgtggaagcc agccgaatcg
    13681 ctcatggcgt accggttggc ggcgaactgg aaatggtcga tggcaccacg ctgtcacact
    13741 cccttgccgg gcgtcataag attcgttttt aagcaaacga gagcaggatc acctgctctc
    13801 gcttgaaatt attctccctt gtccccatct ctcccacatc ctgtttttaa ccttaaaatg
    13861 gcattattga ggtagaccta catgaaagga caagaaactc gtggttttca gtcagaagtg
    13921 aaacagcttc tgcacctgat gatccattct ctctattcca ataaagaaat cttcctgcgt
    13981 gagcttatct ctaacgcctc cgatgcggcg gacaagctgc gtttccgcgc gctctctaac
    14041 ccggacctgt acgaaggtga tggcgaactg cgcgttcgtg tctctttcga taaagacaag
    14101 cgtacgctga ccatctccga taacggcgta gggatgaccc gcgacgaagt gattgaccat
    14161 ctggggacta tcgctaaatc cggcaccaaa tcattcctcg aatccctggg ttctgaccag
    14221 gcgaaagaca gccagctgat cggtcagttt ggtgttggtt tctactctgc gtttatcgtg
    14281 gccgacaaag tgaccgtgcg tactcgcgcg gcaggcgaaa aaccagaaaa cggtgttttc
    14341 tgggaatcgg ctggtgaagg tgaatacacc gttgccgaca tcaccaaaga agatcgtggt
    14401 actgaaatca ccctgcatct gcgtgaaggc gaagacgagt tcctcgatga ctggcgcgtg
    14461 cgttccatca tcagcaaata ctccgaccat atcgcgctgc cggtagagat cgaaaaacgc
    14521 gaagagaaag acggcgaaac cgttatctct tgggaaaaaa tcaacaaagc gcaggcgctg
    14581 tggactcgta acaagtcgga aatcaccgat gaagagtaca aagagttcta caaacacatc
    14641 gcccacgact ttaacgatcc gctgacttgg agccacaacc gcgttgaagg taagcaggag
    14701 tacaccagcc tgctgtacat tccgtctcag gcaccgtggg atatgtggaa ccgcgatcat
    14761 aaacacggtc tgaaactgta tgttcagcgt gtgttcatca tggacgacgc agaacagttc
    14821 atgccgaact atctgcgctt cgtgcgtggt ctgattgact ccagcgatct gccgctgaac
    14881 gtttcccgtg aaatcctcca ggacagcacg gtaacgcgta acctgcgcaa tgcgctgacc
    14941 aagcgtgtgc tgcaaatgct ggaaaaactg gcgaaagacg acgcggaaaa ataccagacc
    15001 ttctggcaac agtttggcct ggtactgaaa gaaggtccgg cggaagattt cgctaaccag
    15061 gaagcgatcg ccaaactgct gcgttttgct tctacccata ccgattcttc tgcgcagacc
    15121 gtgtctctgg aagactacgt ttcccgcatg aaagaagggc aggagaaaat ctactacatt
    15181 accgcagaca gctatgcggc agcgaagagc agcccgcacc tggaactgct gcgtaagaaa
    15241 ggcatcgaag ttctgctgct ttccgaccgc atcgatgagt ggatgatgaa ctatctgact
    15301 gagttcgacg gtaaaccgtt ccagtcggtg tctaaagttg acgagtcgct ggaaaaactg
    15361 gctgacgaag ttgatgagag cgcgaaagaa gcggagaaag cactgactcc gttcatcgac
    15421 cgtgtgaaag ccctgctcgg cgagcgtgtg aaagatgtcc gtctgactca ccgtctgacc
    15481 gatacgccag cgattgtctc taccgatgcg gacgaaatga gcacccagat ggcgaaactg
    15541 ttcgctgcgg cgggccagaa agtgccagaa gtgaaataca tctttgaact gaacccggat
    15601 cacgtactgg tgaaacgtgc ggcagatact gaagatgaag cgaagttcag cgagtgggta
    15661 gaactgctgc tggatcaggc gctgctggca gaacgcggca cgctggaaga tccgaacctg
    15721 tttattcgtc gtatgaacca gctgctggtt tcctgatgtc atgccggatg accttcgtgt
    15781 catccggcat ttttcttttc attatctgca ctttccgcaa attatctcgc cattaaccgt
    15841 ttcagcccca ggtgcctttc ttgaggcaat cgcctgttgg tggtatcgtt tatcgctttt
    15901 tcaaaaaatt cgacacattt taaggggatt ttcgcaatgc gtatcattct gcttggcgct
    15961 ccgggcgcgg ggaaagggac tcaggctcag ttcatcatgg agaaatatgg tattccgcaa
    16021 atctccactg gcgatatgct gcgtgctgcg gtcaaatctg gctccgagct gggtaaacaa
    16081 gcaaaagaca ttatggatgc tggcaaactg gttaccgacg aactggtgat cgcgctggtt
    16141 aaagagcgca ttgctcagga agactgccgt aatggtttcc tgttggacgg cttcccgcgt
    16201 accattccgc aggcagacgc gatgaaagaa gcgggcatca atgttgatta cgttctggaa
    16261 ttcgacgtac cggacgaact gatcgttgac cgtattgtcg gtcgccgcgt tcacgcgccg
    16321 tctggtcgtg tttatcacgt taaattcaac ccgccgaaag tagaaggcaa agacgacgtt
    16381 accggtgaag aactgactac ccgtaaagac gatcaggaag aaaccgtacg taaacgtctg
    16441 gttgaatacc atcagatgac tgcaccgctg atcggctact actccaaaga agcggaagcg
    16501 ggtaacacca aatacgcgaa agttgacggc accaagccgg ttgctgaagt tcgcgctgat
    16561 ctggaaaaaa tcctcggcta atcaagccgc agcagatctg ctttcgggca ggtctgctca
    16621 ttctcctcag gcaatacctc tcacagcaat tagttcttct tcttcacttt tccgctacaa
    16681 ttatcaacaa gttgaatcga taagaggcgg taatgcgtca gactaaaacc ggtatcctgc
    16741 tagcaaacct gggtacgccc gatgccccca cacctgaagc ggtaaaacgc tatctgaaac
    16801 aatttttaag cgacagacgc gtggttgata cctcacggtt gttatggtgg ccattgctgc
    16861 gcggcgtgat tttgccactg cgctcgccgc gtgtggcgaa gctgtatgcc tctgtctgga
    16921 tggagggtgg ctcgccgctg atggtttaca gccgccagca acagcaggcg ctggcacaac
    16981 gtttaccgga gacgcccgta gcgctgggaa tgagctacgg ctcgccatca ctggaaagcg
    17041 ccgtagatga actcctggca gagcatgtag atcatattgt ggtactgccg ctttatccgc
    17101 aatactcctg ttcaacggtc ggtgcggtat gggatgaact ggcacgcatt ctggcgcgca
    17161 aacgtagcat tccggggata tcgtttattc gtgattacgc cgataaccac gattacatta
    17221 atgcactggc gaacagcgta cgtgcttctt tcgctaaaca tggcgaaccg gatctgctgc
    17281 tgctctctta tcatggcatt ccccagcgtt atgcagatga aggcgatgat tatccgcaac
    17341 gttgccgcac aacgactcgc gaactggctt ccgcactggg gatggcaccg gaaaaagtga
    17401 tgatgacctt tcagtcgcgc tttgggcggg aaccctggct gatgccttat accgacgaaa
    17461 cgctgaaaat gctcggagaa aaaggcgtag gtcatattca ggtgatgtgc ccgggctttg
    17521 ctgcggattg tctggagacg ctggaagaga ttgccgagca aaaccgtgag gtcttcctcg
    17581 gtgcgggcgg gaaaaaatat gaatatattc cggcgcttaa tgccactccg gaacatatcg
    17641 agatgatggc taatcttgtt gccgcgtatc gctaaagctg agcggtaaag aactgagcgc
    17701 cgtcgcgaag agcctcgtcg gcggttttca tcatccgtga ataatgcaaa aaggcgtgca
    17761 gcgtgcctgg gtagagtttg aactcacagg gctgctgatg cgccgctaac gtctggtaaa
    17821 gcagacggct gtcatccagc agcggatcga actccgcccc ggcaataaaa cagggcggaa
    17881 cttcgcgagt gagatcatta ttaaacaggc agtaatacgg cgactcgcgg tccgcgtcgt
    17941 tgcttaaata cgcctcttca tacatctgta aatcctgttg cgttaagcca tcccagacac
    18001 cgcccaacag acgacgagtc acggaatccc gtaatccgta aagcccatac cacagcaaaa
    18061 cgcccgcaac tttaccgcaa tcgatctgtt tatcacgcaa ccacaacgca ctggcgagcg
    18121 ccagcatggc acctgcggaa tcaccggcaa agccaatgcg ggacatattg atttgataat
    18181 cctccgcctg ctggtggaaa taacaacaag cagccacaat ttcctctatc gcttgcggaa
    18241 aacgcgcttc aggtgaaagg gtgtaatcaa taccaatcac cgtacattgg ctgtagcttg
    18301 ccagcaggcg catgatgcga tcgtgggtat cgagattgcc gagaataaaa ccgcctccat
    18361 gcaaataaaa tagcgtcgct gggctatctg gctgcggaca aaagagacgt gtttccacct
    18421 gcccatattt tgttggaacc atgtaagctc tggttgccat ttctggagcg cccgcattcc
    18481 agaatcggcg ctcaagcgtg taatactgtc gttgctcagc aatcgttccc gttgcgggcc
    18541 agggcggtaa atccggctga agagtattca caacggtctt catttcagca gaaataaggt
    18601 ccagaacggg taatttgttt tccagcttca tgcaaaaact cctttcaaat tacgtcattg
    18661 taaggaacca ctgccatgaa aatgcgatcc cgcctgctga tattgaaact ggctgcgtct
    18721 cgcgcgctcc cgtcagattg tgttaacatt cgccgctcag ttaaccaccc gtaaaaacaa
    18781 ccatgaaatt tcccggtaaa cgtaaatcca aacattactt ccctgtaaat gcacgcgatc
    18841 cgctgcttca gcaattccag ccagaaaacg aaaccagcgc cgcctgggta gtgggtatcg
    18901 atcaaacgct ggtcgatatt gaagcgaaag tggatgacga attcattgag cgttatggat
    18961 taagcgccgg gcattcactg gtgattgagg atgacgtagc cgaagcgctt tatcaggaac
    19021 taaaacagaa aaacctgatt acccatcaat ttgcgggtgg cactattggt aacaccatgc
    19081 acaactactc ggtgctcgcg gacgaccgtt cggtgctgct gggcgtcatg tgtagcaata
    19141 ttgaaattgg cagctatgcc tatcgttacc tgtgtaacac ctccagccgt accgatctta
    19201 actatctaca aggcgtggat ggcccgattg gtcgttgctt tacgctgatt ggcgagtccg
    19261 gggaacgtac ctttgctatc agccctggcc acatgaacca gctgcgggct gaaagtattc
    19321 cggaagatgt gattgccgga gcctcggcac tggttctcac ttcttatctg gtgcgttgca
    19381 agccgggtga acccatgccg gaagcaacca tgaaagccat tgagtacgcg aagaaatata
    19441 acgtaccggt ggtgctgacg ctgggcacca agtttgtcat tgccgagaat ccgcagtggt
    19501 ggcagcaatt cctcaaagac cacgtttcta tccttgcgat gaacgaagat gaagccgaag
    19561 cgttgaccgg agaaagcgat ccgttgttgg catctgacaa ggcgctggac tgggttgatc
    19621 tggtgctgtg caccgccggg ccaatcggct tgtatatggc gggctttact gaagacgaag
    19681 cgaaacgtaa aacccagcat ccgctgctgc cgggcgctat agcggaattt aaccagtatg
    19741 agtttagccg cgccatgcgc cacaaggatt gccagaatcc gttgcgtgtc tattcgcaca
    19801 ttgcgccgta tatgggcggg ccggaaaaaa tcatgaacac taatggagcg ggggatggcg
    19861 cactggcagc gttgctgcat gacattaccg ccaacagcta ccaccgtagc aacgtaccaa
    19921 actccagcaa acataaattc acctggttga cgtattcgtc attagcgcag gtgtgtaaat
    19981 atgctaaccg tgtgagctat caggtactga accagcattc acctcgttta acgcgcggct
    20041 tgccggagcg tgaagacagc ctggaagagt cttactggga tcgttaagtt atcgtcggtt
    20101 cgtaggccag ataaggcgtt cacgccgcat ctggcatttg gctctcgatg cctgatgcga
    20161 cgctggcgcg tcttatcagg cctacatatc cgcgcctatt atccggcaac caccgtttac
    20221 cccgtcacca cctcacccgc cggtggcgtt tcaagcagtt ccagcatagt acgggcgatt
    20281 tcacgctcac ccatcactac ctgatttgca ccacgttcgg tgatatacgc cacttcatcg
    20341 tcataatggg cgcgggcaat aatctcaata tccggatttt tcgcgcgggc agatgccaca
    20401 atctcacccg cttcataacc gttgggaatc gtcaggatca gccattttgc acattccaga
    20461 tgcgccagtt gcataatttc ttcgttcgcc gcattgccca atactgcgcg gaccccgcgc
    20521 tctcgcagtt catcaacacg ggttcgtgac gtctcaatca ccactaaagg aatatcagag
    20581 gcgagcaatt tctcccccag caggctgcct acacgaccgt aacctaccag tagcgcatgg
    20641 ttgcaaatat ccactgggat ctgcttctct tcttcgatag cctcttccag cgtctgctct
    20701 tccagcgttt cggtcttcgc cagatatttc tccagcagcg caaacaggac cgggttgagc
    20761 ataatcgaca aaatcgcccc tgccagtacc aggttttgtc cggcctgcgg cagtaaattc
    20821 aatgccattc ccagtcccgc caggataaac gcgaactcac caatctgcgc caggctggcg
    20881 gcgatagtta acgccgtgcg ctgggagtga ccaaacagac gcacaaggaa cagtgccgcg
    20941 accgatttac caaacagaat aatcgccagc gtcgccagca ctgccagcgg ttgctgaatc
    21001 agaattaacg gatcaaacaa catcccgacg gagacaaaaa acagcaccgc aaacgcgtcg
    21061 cgcaatggca gcgtatcgtg ggcggcacgg tgactcagtt cagactcgtt cagcaccatc
    21121 ccggcaaaga acgcaccgag tgcaaaggag acatcaaaca gctccaccgc accaaaggca
    21181 atccctaacg ccagcgccag caccgacagg gtaaacagct cacgggaacc ggttgctgcg
    21241 ctgcgtgcca taatccacgg caccagacgg cgacctacca gcatcataat ggcgataaat
    21301 gcgatcacct tgccgatggt gatccccata tcgacagcaa gagtggcaaa gcccacatcg
    21361 ccttgttcca tcattcctgc cactgcgggc agcaacacca gcgtcagaac cattaccagg
    21421 tcttccacaa tcaaccaacc gatggcgatt tgcccacgct gactgtctat taattgccgt
    21481 tcttcaagtg cgcgcagtaa caccacggta ctggcggtgg aaaggcataa gccgaacacg
    21541 ataccggtca ttaacgacca gcccagcacg gcggagagcg ccatacccag cagcgtcgcc
    21601 acggctatct gggcgatcgc accgggaatg gcgatggcct ttaccgccat caaatccttc
    21661 agcgaaaagt gcaaaccgac gccaaacatc aacagaatga cgcccagttc agccagttcc
    21721 ggcgcaagct tggtatcggc aacaaagccc ggagtgaatg gtcctgccag cacacccgct
    21781 aacagatatc ccaccagagg agaaatacgt agtttattgg ccagcatgcc gaggataaag
    21841 gcgagcacaa ggccgccaac aatggtggtg ataagcgggg tggcgtgatg cattccgtct
    21901 ccttttcctg gtggttattg tccatttttg gccgggaaaa ccaaaattac aggtaatagt
    21961 ttatgacaat ttcattgatg atgtttatga ataattgttg aattttgcag aaaaatggaa
    22021 ttagctgcaa aaaaagcacg gatcggaaaa ctgaaggcgg tttaacagag gaaaggctta
    22081 tggcgactgt aaaatgatgc agccaaagtt tgcctttggc tgcaatgaaa tcactcttta
    22141 tgccggttat caggcaggaa tatggtcaac atccccaata gtggcaggaa agcacagatt
    22201 ttatagacta actcgatgct ggtgtggtcg gcgataagcc ccagaactgc cgctcccaga
    22261 cctcccatgc caaaagcaaa accgaaaaag agtccagaaa ccataccgat acgtcccgga
    22321 agcagctcct gagcgtagac cagaatggca gagaatgccg aagcgaggat aaatccaata
    22381 atcaccgtta aaacccccgt ccagtgcagg ctggcgtagg gtaaaatcag cgtaaacggc
    22441 gcaacgccga ggatagagcc ccaaatcaca tattttcgcc caattttatc ccctacaggc
    22501 ccgccgatca ccgtaccggc cgcaacggca aacaggaagg caaacagatg aagctgggca
    22561 ttctggatag ataatccgaa tttttgcatc agataaaagg tgtaatagct gctgatgctc
    22621 gccatataga aatatttcga gaaaatgagg attaacagaa tgctgaccgc cagtaccact
    22681 ttgttgcgcg gcagtggatt gataatcgtc gcttttggtt ttcctttatt cattcggtgc
    22741 tgtgccgagt accaacggct gatttgcgcc aacaccacga tcgccagcag tgccgcaagt
    22801 acaaaccagg ctacgttgcc tttgccataa ggcgcgataa ttaccgctgc cagcaagggc
    22861 cccagggaac tgccaaagtt gccgccgacc tgaaagatag attgcgccag gccatgacgc
    22921 ccgccagaag ccatacgggc cacgcgagaa gattccggat gaaagaccga tgaaccggta
    22981 ccgaccagcg ccgccgccag cagaactgcg ccaaaactgc ccgccagcgc aagcagcacc
    23041 agaccactta aggtaaagca catgccaatt ggcaacgacc acggcatcgg atatttatcg
    23101 gtccagtaac cgaccaccgg ttgcagtagc gaagaggcga gctggaaggt gagggttatc
    23161 atgccaatct gcataaatgt cagagaaaac tctgactgaa gcagcggata aatcgccaga
    23221 atcagggatt ggatcatgtc gttcagcaga tgtgagaggc tgatagcacc taaaatacca
    23281 aacgatgttc gggccttggt cgttgacgca gccgcgcccg ccacaggctg ggtttgttca
    23341 ctcattgcca taggaaagtc actttttcag ggttgcgatg taaagaatga tcttatttgt
    23401 gattattacc cgactaacat acctgtatgc gtcgcctgaa ggaagtctca acgccgaata
    23461 cagaatttct aatctggatg cagatttatc ttcaccggac gcagacttgt ctatgatgtc
    23521 gcgtcatact atttttcaac acgttgaaat caggtcaggg agagaagtat gaaattattg
    23581 cagcggggcg tggcgttagc actgttaacc acatttacac tggcgagtga agctgctctg
    23641 gcgtatgagc aggataaaac ctacaaaatt acagttctgc ataccaatga tcatcatggg
    23701 catttttggc gcaatgaata tggcgaatat ggtctggcgg cgcaaaaaac gctggtggat
    23761 ggtatccgca aagaggttgc ggctgaaggc ggtagcgtgc tgctactttc cggtggcgac
    23821 attaacactg gcgtgcccga gtctgactta caggatgccg aacctgattt tcgcggcatg
    23881 aatctggtgg gctacgatgc gatggcaatc ggtaatcatg aatttgataa cccgctcacc
    23941 gtattacgcc agcaggaaaa gtgggcaaag ttcccgttac tttctgccaa tatctaccag
    24001 aaaagtaccg gcgagcgcct gtttaaacca tgggcgctgt ttaagcgtca ggatctgaaa
    24061 attgccgtta ttggcctgac gacggatgac acagcgaagt taggcaaccc agaaaacttc
    24121 accgatattg agttccgtaa gcccgccgat gaagcgaagc tggtgattca ggagctgcaa
    24181 cagacagaaa agccagacat tattatcgcg gcgacccata tggggcatta cgacaatggt
    24241 gagcacggct ctaacgcacc gggcgatgtg gagatggcgc gcgcgctgcc tgccggatcg
    24301 ctggcgatga tcgtcggtgg tcactcgcaa gctccggtct gcatggcggc agaaaacaaa
    24361 aaacaggtcg attacgtgcc gggtacgcca tgcaaaccgg atcaacaaaa cggcatctgg
    24421 attgtgcagg cgcatgagtg gggcaaatac gtgggacggg ctgattttga gtttcgtaat
    24481 ggcgaaatga aaatggttaa ctaccagctg attccggtga acctgaagaa gaaagtgacc
    24541 tgggaagacg ggaaaagcga gcgcgtgctt tacactcctg aaatcgctga aaaccagcaa
    24601 atgatctcgc tgttatcacc gttccagaac aaaggcaaag cgcagctgga cgtgaaaata
    24661 ggcgaaacca atggtcgtct ggaaggcgat cgtgacaaag tgcgttttgt acagaccaat
    24721 atggggcggt taattctggc agcccaaatg gatcgcactg gtgctgactt tgcggtgatg
    24781 agcggaggcg gaattcgtga ttctatcgaa gcaggcgata tcagctataa aaacgtgctg
    24841 aaagtgcagc cattcggcaa tgtggtggtg tatgccgaca tgaccgggaa agaggtgatt
    24901 gattacctga ccgccgtcgc acaaatgaag ccagattcag gtgcctaccc gcaatttgct
    24961 aacgttagct ttgtggcgaa agacggcaaa ctcaacgacc tgaaaatcaa cggcgaaccg
    25021 gtcgatccgg cgaaaactta ccgcatggcg acattaaact tcaatgccac cggcggtgat
    25081 ggctatccgc gccttgataa caaaccgggc tatgtgaata ccggctttat tgatgccgaa
    25141 gtgctgaaag cgtatatcca gaagagctcg ccgctggatg tgagtgttta tgaaccgaaa
    25201 ggtgaggtga gctggcagta atccgaaagt gccggatatt tgcatccggc acaatgctta
    25261 atcgcggcgg gcgatatcag caaatttggc atcgaggatc tttgccagat cgcctgccgc
    25321 cagttcgata tccagcccgc gcttgccgcc ggaaacataa atagtggcaa attcttgtgc
    25381 gggggcgtcg ataatcgttg gcagacgttt tttctgcccc agtgggctaa tccccccaac
    25441 cagatatccc gtcgaacgct gcgcaaccat cggatcggcc atctcaactt tcttggcacc
    25501 cagcgctttt gctacttttt taagatccag ttgacctgcg accggcgtaa cggccacggc
    25561 aaggtgtttc atatcaccgt tcactgcgac cagcagcgtt ttgtagacct gatccggatt
    25621 caaacctaat tttttgacga cttcatcgcc aaaattggtt tcagccggat cgtgctcgta
    25681 ggtatggatt tgaaacgaaa tcttgttttt ttcgagtaat ttaactgcgg gtgtcatggt
    25741 aattttcctg ccttaaacaa aaaaacgaag caagcatacg cctgatggcg gtccaaaaaa
    25801 aagagtcatc ttgcctaaga gtattggcag gatggtgaga ttgagcgaca atcgagttac
    25861 accgtcgctt aaagtgacgg cataataata aaaaaatgaa attcctcttt gacgggccaa
    25921 tagcgatatt ggccattttt ttagcgcaac atctgcgaca aattcccttc tccatacagg
    25981 tgtagtgcac cgactgcgac cacatagcgc cccggcggca tggcgcataa tttgtcccgc
    26041 caggcgagat ttcgctgatg catcagcaca tcgtacagcg attgactgaa cgtattaggc
    26101 agcgttattt cattattttg cggcggcgca ttcagccacc agctcatcat ttgttgcagc
    26161 aaccgtgcat tggtgtgcca gtgggtcagc gtatcgtcca gcagtgccag tccgttgtca
    26221 gggagctgga gcaacatggc aatctggttt tcagcccctt ccagttcaat cacgggttta
    26281 tgttgttgct tcgccgcctg caatagctgg taatcgatac cgtattctgc ccgcagcccc
    26341 agtttttgtg cctgcgtcgc ctgaagaacc atcgcgattt gccacagcgg ttgggtagaa
    26401 aagagtgagg tagaaatgcc catctcctgg ctaatgtgct gcaggttttg gagttgctcc
    26461 tcgctaatgc gctcttccag cgcctcgcag gcaggcaaat tagcaaaagg cgtatcgctg
    26521 gtggaaacat ccgcctcgac gatcagcgca tcggcgtttt tgagcttttt gagcaaacgg
    26581 gtgggcaggg gagccatatc gtggctgccc atatgaatac tgccaatcag atgaaaatgg
    26641 cgattgccgg gaagagagat atcgatagca ggccaggtgt aatgattacc gcgcagcgcg
    26701 gcccaaagcg tttttacccg gtacaacaga tccatacgac ctccctttgt gaaatatcat
    26761 gctagcgcgc ggggagggat ggcgcaaatg gagttatgag gcttgttcag tggtagctta
    26821 aatatgatcg aaaaattgtt tttgctttga aacgaaagac tgcaccggtg ttggtggcgt
    26881 caaaatcgcc gaggcgttcc ctgaaggccg gggcagccca catggatgtg ggctgagggc
    26941 gcgttttaca gggatgttac ctcccgcccg acccggtagc cgcaagggat aagctgaggg
    27001 taccgcgcag cggcgatttt gttcgccaga gcccgggggt gcagggggcg gcggcgattg
    27061 gccgccccct gcgcgctccc tgcgccagtc actttatatg gcattactca taaaaggagc
    27121 gcaacaagaa ctggatctca caaacgcatt aagtaaatac caatcattcc ttcggtttaa
    27181 accgcagcaa ccggttggcg ttactcacta cggtaatcga cgagagcgcc attgccgctc
    27241 cggcgaccac cgggttaagc agtgttccag tgaacggcca caaaataccg gcggcgaccg
    27301 gaataccgat actgttgtag ataaacgcgc cgagcaggtt ctgcttcata ttgcgcagcg
    27361 ttgcgcggga aatcgcgagc gcatccgcaa cacccatcag gctatggcgc atcagggtaa
    27421 tcgccgcggt ttcaatggca acatcactgc cgccacccat cgcaataccg acatccgcct
    27481 gagccagcgc tggcgcgtcg ttaatgccgt cgcccaccat tgccacctga cgtccttcac
    27541 tttgcagacg tttgatcgct tcggctttac cgtccggtag caccccggct atcacctcat
    27601 caatccctgc ttctttggcg atcgcattgg cggtggttgg gttatccccg gttagcatca
    27661 ccagacgata tcccgcttta tgcaggcgtt gcagcgccgc cacgctatca ctacgcaatg
    27721 gatcgcgtac agccagcagg gctaccgctt tcccgtcaac cgccagcagc acaggcgttg
    27781 ccccttgtga tgcctgagct gaaatatctg cctcaatcgc tttagtatca acttgctggt
    27841 catttaacag cgcctgattg cccagcaata ccgcatgacc ttcagcttca ccgctcacac
    27901 ccagcccgcg caatgtgcgg aaaccgttga cctgcggtag ctgcatatca cctgctttat
    27961 cgaggatcgc ccgtgccagc gggtggctgg aaccttgctc cagcgccgcc gccagacgca
    28021 atgcctgcgc ttcatcaaca tcagcaaatg tttttactgc gacaacctgc ggcttccctt
    28081 cggtcagcgt cccggtttta tcgaacacca cggtgtcgag cgtactggcg cgttgcagcg
    28141 cgtcggcgtc ccgcaccagc acaccaaact cagccgcccg cccgacgccc gaaataatcg
    28201 acatcggcgt cgccagtccc agcgcacacg gacaggcgat aatcagcacc gtggtggcaa
    28261 tcaccagggt atagacaatc tgcggtgccg gaccaaagaa ataccagatt gccgcactga
    28321 tcagcgcaat gactaccact accggcacaa acacggcgga gattttatcc gccagttgac
    28381 cgatttctgg cttgctgctc tgggcctgtc gcaccatgcg aatgatccgt gacagcgtag
    28441 tatggctgcc gaccgcactg gcacgaaaca gcacactgcc gtcctgtacc actgtcccgg
    28501 catggacgct atcgccttcg cctttttgct gcgggatagg ttcgcccgtc agcatcgctt
    28561 catccagcca cgcttcgcct tgggtaatct caccatctac cggcacacgg tcgccggtcg
    28621 tcaggcgcag taacatacct ggctgaacat ctgccagcgg cacgtttttt tcaccttcgt
    28681 cggtgaccag gcgcgccgtc ggtggggtta aatcgagtaa tttctccagc gccttagaag
    28741 aacgctgacg cgcgcgcgct tccagcatat ggccgagatt gatcagaccg ataatcatcg
    28801 cgctggcttc gtaataaaga tgtcgcgctt ccatcgggaa ccactgcggc cacaggttga
    28861 cgctcatcga atagagccac gccacgccag tacctagcgc caccagcgta tccatcgtcg
    28921 ctgcaccgtt cagcaggctt ttccatgcac tacggtaaaa atggccgccg gcgaaaacca
    28981 tcactgccag ggttatcagg ccgataacta accacaagct gcggttgtca gcggtgacca
    29041 tcatgttatc gccgagcatc ccccagacca tcaccgggat acccaccgcc agtgcgacaa
    29101 ttgcctgcca gcggaagcgc ttcatcgtag cgactgcggt ttcctgctgg cgctcgcggc
    29161 gtttagcgtc atcttcaatc gcttccgcgc cgtagcccgc tttttccacc gcctgcacta
    29221 aatcttgtgg ggaggcactg cccatcacca gcgcagtacg ctccgccagg tttacccgtg
    29281 cctgagtgac gcccggtacg ctttgcaacg cattttgtac gcgggtgaca cagctggcgc
    29341 agctcatgcc gctcagcagc aactgctggc tgtcatcgtc atcggcggtc gctgccggaa
    29401 gcgcctcaga aaccgctgtc agtgcttccg acgggattga tgactccgcc agcggtttag
    29461 cctttgggtg gcttacagat gcgtcataac ccgcttgttt gatggtttcg atcagctgtt
    29521 ctgcactggc agtcccggta acgtgcgctt cagtgacaga cacatccgcc tgctcaacat
    29581 ccgggcgctg ttcgagactt tctttcacgc gtttaacgca gtgaccgcag gacaggccgt
    29641 ccagggtcag gtcgatagtt tgtgacataa aacactcctt ttaagacagt tttgactggc
    29701 tgtgataaag gttaaacctt ccagcaaggg gaaggtcaag aaattaataa accaggcggg
    29761 taaaagtccg taaagattaa aaaatcggct cgatttgcat caggattaga catttatctc
    29821 tttgttttcc tgtagttaag ttgcgggtgc taagttaaat caggatgcct gaaaatcggc
    29881 accggggtga ggaattacct cccgcatcta taaaaaggag ttaacaaaag atgttagatg
    29941 caaacaaatt acagcaggca gtggatcagg cttacaccca atttcactca cttaacggcg
    30001 gacaaaatgc cgactacatt ccctttctgg cgaatgtacc tggtcaactg gcagcagtgg
    30061 ctattgtgac ctgcgatggc aacatctatc gcgcgggtga tagtgattac cgttttgcgc
    30121 tggaatccat ctcgaaagtc tgtacgttag cccttgcgtt agaagatgtc ggcccgcagg
    30181 cggtacagga caaagttggc gctgacccga ccggattgcc ctttaactca gttatcgcct
    30241 tagagttgca tggcggcaaa ccgctgtcgc cactggtaaa tgctggcgct attgccacca
    30301 ccagcctgat taacgctgaa aatgccgaac aacgctggca gcgaatttta catatccaac
    30361 aacaactggc tggtgagcag gtagcgctct ctgacgaagt caaccagtcg gaacaaacaa
    30421 ccaacttcca taaccgggcc attgcctggc tgctgtactc cgccggatat ctctattgtg
    30481 atgcaatgga agcctgtgac gtgtacaccc gtcagtgctc cacgctcatc aatactgttg
    30541 aactggcaac gcttggcgcg acgctggcgg cgggtggtgt gaatccgctg actcataagc
    30601 gcgttcttca ggccgacaac gtgccgtaca ttctggctga aatgatgatg gaagggctgt
    30661 atggtcgctc cggtgactgg gcgtatcgcg ttggtttacc gggcaaaagc ggtgtaggtg
    30721 gcggtattct ggcggtcgtc cctggcgtga tgggaattgc cgcgttctca ccaccgctgg
    30781 acgaagaagg caacagtgtt cgcggtcaaa aaatggtggc atcggtcgct aagcaactcg
    30841 gctataacgt gtttaagggc tgatcatgat gaacacggaa ggtaataacg gtaacaaacc
    30901 cctcggtcta tggaacgtcg tttccatcgg tattggggca atggtggggg cggggatctt
    30961 cgcgctgctg gggcaggctg cgttgctaat ggaagcctcg acctgggtcg cctttgcatt
    31021 tggcggtatt gtggcgatgt tttccggtta tgcctatgcg cgcctggggg cgagctatcc
    31081 cagcaatggc ggtattatcg acttctttcg tcgcggatta ggcaacggcg tcttttcgct
    31141 ggcgctctcg ttactgtacc tgttgacgct ggcggtgagc atcgccatgg tcgcccgtgc
    31201 ttttggcgct tatgccgtgc agtttttgca tgaaggcagc caggaggagc accttatatt
    31261 gctctacgcg ttggggatca ttgcggtgat gacgcttttc aactccttaa gcaaccatgc
    31321 ggtagggcgg ctggaagtga tcctcgtcgg cattaaaatg atgatcctgt tattgctgat
    31381 tattgccggt gtctggtcgc tgcaaccggc gcatatttct gtctctgcgc cccccagctc
    31441 cggtgcgttc ttctcctgta tcgggataac cttccttgcc tatgcgggct ttggcatgat
    31501 ggcgaacgcg gcggataaag tgaaagatcc gcaggtcatt atgccacggg cgtttctggt
    31561 ggcgattggc gttaccacgt tgctttatat ctcgctggcg ctggttttgc ttagcgatgt
    31621 atcggcttta gagttagaaa aatatgccga taccgccgta gcgcaggcag cttctccgct
    31681 gctcgggcat gtgggttatg tgatcgtcgt catcggcgct ttactggcga cggcttcagc
    31741 catcaacgcg aacctgttcg ccgtgtttaa catcatggac aacatgggca gcgaacgcga
    31801 actgccgaag ctaatgaata aatccctgtg gcggcagagt acctggggca acatcattgt
    31861 cgtggtgctg attatgctga tgacggcggc gctgaattta ggctcactcg ccagcgttgc
    31921 cagcgccacc tttttgatct gctatctggc ggtgtttgtg gtggcgatcc gcctgcgtca
    31981 tgatattcac gccttgttgc cgattcttat cgttggtacg ttggtgatgt tgttggtgat
    32041 cattggcttt atctacagcc tgtggtccca gggcagccgt gcgttgatat ggattattgg
    32101 tgcaatctta ctcagcctta ttgtggcaat ggtcatgaag cgcaataaaa ccgtataaca
    32161 cctctctgtg cgcagtacag cctgtactgc gcctttgtct ttaattcaaa ttacctttct
    32221 tattccttta ataaataagt cacaaaaaat gatatatacg cgataaatct gtgatgct
//
LOCUS       DACBVI010000041        31161 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.041, whole genome shotgun sequence.
ACCESSION   DACBVI010000041 DACBVI010000000
VERSION     DACBVI010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.041"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            248..631
                     /gene="secE"
                     /locus_tag="IU325_09160"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="IU325_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP5809195.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="IU325_09165"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="IU325_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP5809196.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="IU325_09170"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="IU325_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP5809197.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="IU325_09175"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="IU325_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP5809198.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="IU325_09180"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="IU325_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP5809199.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="IU325_09185"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="IU325_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP5809200.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="IU325_09190"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="IU325_09190"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP5809201.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="IU325_09195"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="IU325_09195"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP5809202.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12676..13215
                     /locus_tag="IU325_09200"
     CDS             12676..13215
                     /locus_tag="IU325_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
                     /protein_id="HAP5809203.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARIHLKRTILALPES
                     NVIHAGYGSYAVIEVDLNGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13625..14758)
                     /gene="thiH"
                     /locus_tag="IU325_09205"
     CDS             complement(13625..14758)
                     /gene="thiH"
                     /locus_tag="IU325_09205"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HAP5809204.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINSKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRP"
     gene            complement(14755..15525)
                     /locus_tag="IU325_09210"
     CDS             complement(14755..15525)
                     /locus_tag="IU325_09210"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HAP5809205.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVESIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAIADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15527..15727)
                     /gene="thiS"
                     /locus_tag="IU325_09215"
     CDS             complement(15527..15727)
                     /gene="thiS"
                     /locus_tag="IU325_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HAP5809206.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15711..16466)
                     /gene="thiF"
                     /locus_tag="IU325_09220"
     CDS             complement(15711..16466)
                     /gene="thiF"
                     /locus_tag="IU325_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HAP5809207.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINTACVALNTPLITASAAGFGGQLMV
                     LTPPWEQGCYRCLWPDTQDPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRATGCPVCGGSNADPV"
     gene            complement(16459..17094)
                     /gene="thiE"
                     /locus_tag="IU325_09225"
     CDS             complement(16459..17094)
                     /gene="thiE"
                     /locus_tag="IU325_09225"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAP5809208.1"
                     /translation="MYQPDFPPVPFRLGLYPVVGSAQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAITLGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLSAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVMATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17094..18989)
                     /gene="thiC"
                     /locus_tag="IU325_09230"
     CDS             complement(17094..18989)
                     /gene="thiC"
                     /locus_tag="IU325_09230"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HAP5809209.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTQPGVRV
                     PMREIQLSPTLIGGSKEQPQFEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGLLAPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSVQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19070..19170)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19223..19699)
                     /locus_tag="IU325_09235"
     CDS             complement(19223..19699)
                     /locus_tag="IU325_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rsd/AlgQ family anti-sigma factor"
                     /protein_id="HAP5809210.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLVRAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19794..20567
                     /gene="nudC"
                     /locus_tag="IU325_09240"
     CDS             19794..20567
                     /gene="nudC"
                     /locus_tag="IU325_09240"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HAP5809211.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGEIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20607..21671
                     /gene="hemE"
                     /locus_tag="IU325_09245"
     CDS             20607..21671
                     /gene="hemE"
                     /locus_tag="IU325_09245"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HAP5809212.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21681..22352
                     /locus_tag="IU325_09250"
     CDS             21681..22352
                     /locus_tag="IU325_09250"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HAP5809213.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22395..22985
                     /locus_tag="IU325_09255"
     CDS             22395..22985
                     /locus_tag="IU325_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF416 family protein"
                     /protein_id="HAP5809214.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAVFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23172..23444
                     /gene="hupA"
                     /locus_tag="IU325_09260"
     CDS             23172..23444
                     /gene="hupA"
                     /locus_tag="IU325_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HAP5809215.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23457..24152
                     /locus_tag="IU325_09265"
     CDS             23457..24152
                     /locus_tag="IU325_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HAP5809216.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24154..24579)
                     /gene="zraP"
                     /locus_tag="IU325_09270"
     CDS             complement(24154..24579)
                     /gene="zraP"
                     /locus_tag="IU325_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HAP5809217.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24817..26214
                     /gene="zraS"
                     /locus_tag="IU325_09275"
     CDS             24817..26214
                     /gene="zraS"
                     /locus_tag="IU325_09275"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HAP5809218.1"
                     /translation="MRFMQRSKDTLAKWLSAILPVVIVGLVGLFAVTVIRDYGRASEA
                     DRQALLEKGNVLIRALESGSRVGMGMRMHHVQQQALLEEMAGQPGVLWFAVTDAQGII
                     ILHSNPEMVGRALYSPDEMQQLKPEENSRWRLLEDAEKTPALEVYRLFQPMSLPWRHG
                     MHNMPRCNGKAVPQAEAQQAIFIAVDASDLVATQSGEKRNTLIILFALATVLLASVLS
                     FFWYRRYLRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPA
                     GGEAHQLAQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANS
                     REIQLRFTANDMLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKIS
                     VTDSGKGIAADQLEAIFTPYFTTKADGTGLGLAVVHNIVEQHGGTIQVASQEGEGATF
                     TLWLPVNITRKDPQG"
     gene            26211..27536
                     /gene="zraR"
                     /locus_tag="IU325_09280"
     CDS             26211..27536
                     /gene="zraR"
                     /locus_tag="IU325_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HAP5809219.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGQQALAQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHMHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LAQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27533..28822)
                     /gene="purD"
                     /locus_tag="IU325_09285"
     CDS             complement(27533..28822)
                     /gene="purD"
                     /locus_tag="IU325_09285"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP5809220.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHQIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GNKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVSDGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28834..30423)
                     /gene="purH"
                     /locus_tag="IU325_09290"
     CDS             complement(28834..30423)
                     /gene="purH"
                     /locus_tag="IU325_09290"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP5809221.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DANEGSLLLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
     gene            31038..>31161
                     /locus_tag="IU325_09295"
     rRNA            31038..>31161
                     /locus_tag="IU325_09295"
                     /product="16S ribosomal RNA"
BASE COUNT         7379 a         8040 c         8516 g         7226 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaatac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggt agcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacctggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcaccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gcgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca tggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agactccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggctgg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgcgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg ctctgctgaa
     1861 agaactggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaggg tgcaaacgct gaagctgcta aagctgcagg
     2041 tgctgaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgc ggcctgatgc caaacccgaa agtgggtact gtaacgccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccag gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagctgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgta tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtattga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgtgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgacctggat
     5161 cacggcccgt atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgctatg acctgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat cctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc attgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gtggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ctgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatct
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaacatgc aacgtcaggc cgttccgact ctgcgtgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggttgctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctataac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggtagaacg tggcgacgtg
     6481 ctggcagatg gtccgtctac cgacctcggt gaactggcgc tgggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg ggccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtaaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcgtctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 accgacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttctggtca gatccgcctg tacgacggac gcactggtga acagttcgaa
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcacc gttaagtctg atgacgtgaa cggtcgtact
     8041 aagatgtata aaaacatcgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggtgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggtca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggcctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact tcgaatccta tgtggttatt gaaggcggta tgaccaacct
     8701 ggaacgccag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaaaatgg gtgcggaagc aatccaggcc ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaactaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcttgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgatatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggcctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggta tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gccaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg caggtatgcg tggtctgatg gcgaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgcgcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgaa ggtggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatatcctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 aaactctgtc gacgcggtta aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcattaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catcacgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagta ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattacg cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc ctggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacaccc tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggatct gttcgaagca cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc caaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgatggtcc
    11881 ggaagcgcca cacgacattc tgcgtctgcg tggtgttcat gctgttactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgttaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ctggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggggtag tttagggaaa gagcatttgt
    12541 cagaatattt aatgaatttc tggatactca taatcaatgt agagatgact aatatcctga
    12601 aactgactga actaattgag tcaagctcgg caagaattcg atactattcc tgtgtgactt
    12661 tcttaaggaa cgataatgaa acaggaagtg gagaagtggc gaccttttgg acatccggat
    12721 ggtgatattc gtgatttatc atttcttgat gctcatcagg ctgtctacgt tcagcatcat
    12781 gagggcaaag agcctttaga gtatcgcttc tgggttacct actctcttca ctgcttcaca
    12841 aaagattatg aacatcagac taacgaagaa aaacaatcgt taatgtacca cgcgcctaaa
    12901 gaatctcgtc ccttctgcca gcaccgttat aacttagcgc gcatacactt aaaaagaacc
    12961 attttggcgc tgccagaaag caacgttatt catgctgggt atggtagcta tgccgtgatt
    13021 gaagtggatt taaacggagg agacaaggca ttttactttg ttgcgttcag ggctttcagg
    13081 gaaaagaaaa aacttcgttt gcatgtaact agcgcttatc ccatttctga aaaacagaaa
    13141 ggtaaatcag tgaaattttt caccattgcc tacaacttat tgagaaataa gcagcttcct
    13201 cagccctcaa aataacaaaa cccaccttaa ggtgggtttc gccagagaat tatctctggt
    13261 attcagaacg ccattaccgg actttgcctt gaccttgcga taatcgcagg ttgcgggatg
    13321 tctgaatttc ttcagtctgc tgcatcctgg aagatgagaa catgtgttct tattttcgtc
    13381 tctatcatag ttgagtattt actctcttac aatcagatct ctttcattgc tcaacaggcg
    13441 atggcttcag actttgcatt acggaatttt taagaaaggc agggcgaaac gaggaagaag
    13501 cttttcgccc tggacaacat tcctgctgac ggcactacca taaaaaaaca caacgctttc
    13561 aacaaccttg cgtttttcat cttccgtagc ggctcggaat aaatttacgt tcttacattc
    13621 cgtctcatgg cctttgcgag gcgcgtccca gatagctgtc ccagtctttc cataccggct
    13681 gcaaaccctg agcggttaac gcggcagcaa ccgcttccgg tctgcgatcg tcgtgtggtg
    13741 agaactgttc cagctcgggg tgattgtcgg catagccacc tggctgcgtt ttcgagaaag
    13801 cgctgacgtt attaattgcc agcggaatca cgcgatcgcg aaaccacggt gattcccgcg
    13861 tggagagtga cagttcaatc tccggtgcaa gcagccggaa ggcgcagatg gtttgcacta
    13921 actggcgttc atccataatc gacgcaggct caatgccgcc agtacatgga cgcaggcgtg
    13981 ggaaggagac agagtagcgg ctttgccagt aatgctgttg cagccatagc aaatgttctg
    14041 caaccatata gcagtcaacg cgccagctgt cggaaaggcc aattagcgcg ccgaggccta
    14101 tcttatcaat ccccgcacgg cccagccgat ccggcgtttc cagccgccag aagaagtcct
    14161 gttttttgcc tttcagatgg tggcgggcat aagtcgcctc gtgatatgtc tcctgataaa
    14221 ccatcacgcc atccagacca agttgcttta actcggcgta ttccgtctcc gccagcggct
    14281 gtacttccat ctgtagtgaa gagaactgtt cacgcagggc agggagatga cgacgaaagt
    14341 aatccatccc cactttcgcc tgatgttcac cagtgactaa aagcagatgt tcaaagccca
    14401 tctcccgtat agcggcgctt tccctggcaa tatccgcttc atccagcgtt ttgcgcttga
    14461 tgcggttgct catagagaat ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat
    14521 aaagcgggac gtagaagcta acagtgttgc caaatcgttg acgggtcaga cgctgcgccc
    14581 gttgggccag tggttccaga tagccactgg cggctggcga taacagcgcc atcatatcgt
    14641 cgcgggtgag ttgcgaggca tttagcgccc gctctacgtc agcagccgtt ttgctgttga
    14701 tacgcaggcg gatgtcgtcc cagtccagtt gtcgccagcg atcgctgaag gttttcatgc
    14761 cgatgcctcc agaaatccgg tcagcgggct ggtggcatga gcaaaatgac tgcggctgcc
    14821 cggtccggac tgacgtgcca gcaggcctgc ttctactgcc agacgaaatg ccttcgccat
    14881 gttgacggga tcgtccgcga tggcaatcgc cgtattcact aacaccgcgt cggcccccat
    14941 ttccagcgcc tgcgcggcat ggctgggaac gccgatgcca gcgtcgacaa ccaccggaac
    15001 cgtggcttgc tggataataa tctccagcat ggcacgggtt tccagtccct gattcgagcc
    15061 aatcggcgcg ccgagcggca tcaccgctgc acagccgact tcttccagac gtttacacaa
    15121 taccggatcg gccccgcagt aaggcagcac gacaaatccc tgttgtacca gcgtttcggc
    15181 ggctttcagg gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt gaatctctaa
    15241 ttttagccag tttgtgccta atgcttcacg tgccagatgg gcggcgaaaa tggcttcttc
    15301 cgctgttttc gccccggatg tattcggtag cagggtcaca cccgccgcga taagcggttc
    15361 gaggatagcg tcgttgtgct ggcgcaagtc gacacgtttc atcgccagcg tcaccagttg
    15421 gctgccggaa gcgcgaatag actccaccat cagttgtgat gaggcgaatt tcccggtgcc
    15481 ggtaaacaga tgtgaatcaa acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa
    15541 taacctgaaa aagcaggatc tggtcgccat cctgcacgat atgttgcgcc cactgctcac
    15601 gcgggacgat ttgctgatta atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct
    15661 ccagtagttc gtgaaccgtt tgcccggcgg cgcactgcat cggttgatcg ttaaacagga
    15721 tctgcattgc ttcctccgca taccgggcaa cctgtagcgc ggcgcagcgc caggctgcgc
    15781 cactggctag acttaccgtc gaacagtcgg agttctcccg caggcgtctc tataccgctt
    15841 aacaacttaa tggcttccag tgcctgcaaa gtgcccataa ccccgaccac cgggccaacc
    15901 acgcccgccg tgcggcagtt gcgttctgga tcctgggtat ctggccacag gcagcggtaa
    15961 cacccctgct cccagggcgg cgtcagtacc atcaactgac cgccaaatcc ggccgcgctg
    16021 gcggtgataa gcggcgtgtt gagtgccacg caggcggtat taatctcctg acgagtcgcc
    16081 atattgtcgg tacagtcgag caccacatcg gcccgtgcta ccgcatcttt taacgcctca
    16141 cccgttaacc gttgttgtaa tgctgtcagt tgaatatcgg gattcaactg tgtcagtcgc
    16201 tgatggctga cctgcgattt cgggcgatcg atatcttcag tggtaaagag gatttgtcgt
    16261 tgcagattgc ttaaatgcac atcgtcgtca tctgccagta ccagcgtccc gacgccagcg
    16321 cccgccaggt atagcgcagc aggtgtaccc agcccgccaa gaccaataat cagcacctgg
    16381 ctgtcgagca gtttttgctg cccgtcaaga gcgatatcgt cgagcaggat ttggcggcta
    16441 taacgcataa agtcacggtc attcatcgcc aactcctgca atttccagca actgtgccgt
    16501 tgccaaacgc cagtctgcgg cttgagtaat ggcgctgaca acggcgatac tgccgacacc
    16561 cgtcgccatt accgcaggcg cgcgcgccag actgataccg ccaatcgcca cggtgggata
    16621 atccgccagg cgctcaacat gccgtgccag ctgttccagc ccctgcggtg cagaaggcat
    16681 ctgtttggtt tgcgtcggga acacatgtcc cagcgcgata taagaggggc gtgctgccag
    16741 cgcgacgtcg atctccatat cgtcatgggt cgaaacgccc agtcgcagac ctgccgcgcg
    16801 gatggcgctg agatcggtgg cttgcaaatc ttcctgcccc agatggacgc catacgcctg
    16861 atgcttgatc gccagccgcc agtaatcgtt gataaacaat cgcgcgttat agcggcggcc
    16921 cagcgtaatt gccgccacga catcggcttc cacctcttca tcgcgccgat ctttgatgcg
    16981 tagctggaga gtacgcacgc ctgcatccaa cagacgttcg atccactgtg cgctgcccac
    17041 caccgggtac agtcctaaac gaaaaggtac aggaggaaaa tcaggctgat acatcacgct
    17101 tcctccttac gcaggtagat ttctccgcct ctggcgcgga agttctccga catatccgcc
    17161 attcccactt caatggtttg cgcggcggcg taatcacgca cttcctggct gattttcatc
    17221 gagcagaatt tcggcccaca catggagcaa aaatgggcga ctttaccgga ctcttgcggc
    17281 agggtttcat cgtgataagc gcgggcagta aacgggtcga gggccagatt aaactggtct
    17341 tcccagcgaa attcgaagcg ggctttcgac atggcgttat cgcgaatttg cgcgcccgga
    17401 tgccctttcg ccaggtcagc ggcgtgcgcg gcaatcttat aggtgataag cccctgctta
    17461 acatcttctt tattaggcag acccagatgc tcttttggcg ttacgtaaca gagcatcgcg
    17521 cagccaaacc agccaatcat cgccgcacca atccccgacg tgaagtggtc ataacccggc
    17581 gcgatatcgg tagttagcgg ccccagagtg taaaacggcg cttcgtggca gtgctctaac
    17641 tcctcggtca tattgcggcg gatcatctgc atcggcacgt ggcctgggcc ttcaatcatc
    17701 acctgtacgt catattccca ggcgattttg gtcagttcac ccagcgtatg cagctcggca
    17761 aattgcgctt catcgttggc gtcctgaaca gaaccggggc gcagaccgtc gcccagcgac
    17821 agcgaaacgt cataagcggc acagatttca caaatttcgc ggaagtgttg atagaggaaa
    17881 ttttcctgat gatgggagag gcaccatttc gccataatcg aaccgccgcg agagacgata
    17941 ccagtcaggc gtttcgcggt catcggcaca tagcgcagca gtacgcccgc atggatagtg
    18001 aagtaatcca caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta
    18061 agatcttcgg cgatcccgtt aaccttctcc agcgcctggt agatcggcac tgtaccgatc
    18121 ggcaccgggc tgttacgcaa aatccactcg cgggtttcgt gaatatagcg accggtggag
    18181 agatccatca ccgtatccgc tccccagcgc gtggaccata ccagcttttc cacttcttct
    18241 tcgatggaag aggtgaccgc cgagttgccg atattggcgt taacttttac caggaaattg
    18301 cgaccaataa tcatcggctc cgattccgga tgattaatgt tggccgggat aatcgcgcgt
    18361 ccggcagcaa cttcatcacg gacaaattcc gcagtgatat tttccggcag acgtgcgcca
    18421 aagctcattc ccggatgctg gtggcgtaaa acttcgctac gtatgcgctc gcggcccata
    18481 ttctcgcgga tggcgatgaa ttccatttcc ggtgtgatga tgccctggcg ggcgtagtgc
    18541 agttgggtga cgcggcgtcc tgctttggcg cgttttggcg ctaataagcc gctaaaacgt
    18601 agctcgtcga ggccatcatc tgccagtcgc gctttagtgt aatcggaact gcgcacggta
    18661 agttcttcgg tatcgccgcg cgcatcgatc cacggctggc gtagttttgc cagtccttgc
    18721 tgcacgttaa tggcgatctg cggatcacca tacgggccgg aggtgtcgta gaccggaatc
    18781 gcttcgtttt cttcaaactg cggctgttct ttgctaccgc caattagcgt cgggctaagc
    18841 tggatctcac gcatcggcac gcgcacgccg ggttgtgtgc cagtgatata aatgcgtttt
    18901 gagttgggaa aggcggtgcc ttccagggtg tcgataaaat gttgggcctg ggcgcgttgt
    18961 tcgcggcggg tcagttttgt tgcagacata gctcattcca aaaagttaag gacgtggctt
    19021 gtcagacgac ggatgaagca agagacgatc gccgcaaagg cgatgcgata gcagattaac
    19081 tcttgttccc ttcgcaggta ttagcctgat caggttccgc ggatcccgaa taaacggtct
    19141 cagccagtta aggcactccg acaagaaatt agcccccaca aacggggcgt tgaatgtaaa
    19201 ttacgcgtta acagcgcaga actcaagcag gatgtttgac gcgggcggcg tcaagcacca
    19261 gcagaatcag cttatcttcc agcacaaagc gcgcttccag cgcctcgccg atgtcagata
    19321 aaacctgttg aaactcaagg taattatcat gatcgatggc ggtttccagg ctggaatcgt
    19381 agtaatccat aatctgttgg gtattggctt cgagttgcgg ccaaatcttc gcggcgcgca
    19441 cgagttgccc gttgccttcc agcttatgaa gaatgcgctc ataaatactg aaatgtccgg
    19501 cagacaagta atcgaccagg ctctgacaaa aatcatcaag ggctttttcg tttagcctca
    19561 tgtacgattc tttgccaggc ttaatgccaa ccagattgta gtaagccacg agcagatgct
    19621 tacgtacatg tagccagcga tcaaccagtt tgttacttcc tctgacgcgt tccgtcaggt
    19681 tatcgagctg gttaagcatg attgactccg caagtttgta ttcaaaaact gctcagtgag
    19741 aaatgtaaaa accatgttaa acatgccagt gatgcaaagg tagtgcaaga gctatggatc
    19801 gtataattga aaaattagat cacggctggt gggtcgtcag ccatgaacaa aaattatggt
    19861 tgccgaaggg agaattgcca tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg
    19921 cactacaaat cggcgaatgg cagggggaac ctgtttggtt agtacaacag cagcggcgtc
    19981 acgatatggg gtcggtacgt caggtcattg atctcgatgt tgggctgttt caactggccg
    20041 gacgaggcgt acaactggcg gagttttacc gatcgcataa atactgtggt tactgcgggc
    20101 atgaaatgta tccgagcaaa accgaatggg cgatgctgtg cagccattgc cgtgagcgtt
    20161 actatccgca aatcgccccc tgcattattg ttgccatccg tcgcgatgat tcgatcctcc
    20221 ttgcccagca tacccgccat cgtaacggtg tccatacggt acttgccgga ttcgtcgaag
    20281 tgggcgaaac cctcgagcag gcggtcgcgc gggaagtgat ggaagagagc ggaattaaag
    20341 ttaaaaactt gcgttatgtg acttcccagc cgtggccgtt tcctcagtct ttaatgaccg
    20401 cgtttatggc ggaatacgac agcggcgaga tcgttatcga cccgaaagaa ttgctcgagg
    20461 cgaactggta tcgctatgac gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc
    20521 gtctgataga agatacggtg gcgatgtgtc gggcagagta tgagtgatga tacactgacc
    20581 gcctgacgca ctaaggaaca gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg
    20641 cgctgctgcg ccagcccgtt gatgtcactc cagtatggat gatgcgccag gcgggtcgct
    20701 atctaccgga atataaagcc acgcgcgccc aggcgggcga ttttatgtca ctgtgcaaaa
    20761 acgccgagct ggcgtgcgaa gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg
    20821 cgatcctctt ttccgatatc ctcaccgtgc cggacgcgat ggggttaggg ctctattttg
    20881 aagccggaga aggtccgcgt tttacctcgc cagtcacctg caaagccgac gtcgataaac
    20941 tgccaattcc ggacccggaa gatgagctgg gttacgtgat gaacgcggtg cgtaccattc
    21001 gtcgcgaact gaaaggcgaa gtgccgctga ttggtttttc cggcagcccg tggacgctgg
    21061 cgacctatat ggtggaaggc ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt
    21121 atgccgatcc gcaggcgctg cacgcgctac tcgataaact ggcgaaaagc gtcactttgt
    21181 atctgaatgc gcagattaaa gccggtgctc aggcagtgat gattttcgac acctggggcg
    21241 gcgtgcttac cgggcgcgat tatcaacagt tctcgcttta ttacatgcat aaaattgttg
    21301 atggtttact gcgtgaaaac gacggtcgcc gcgtaccggt cacgctgttt accaaaggcg
    21361 gcggacagtg gctggaagcc atggcagaaa ccggttgcga tgcgctgggc ctcgactgga
    21421 caacggacat cgccgatgcg cgccgccgcg tgggcaataa agtcgcgttg cagggtaata
    21481 tggatccgtc gatgctgtac gcgccgccag cccgcattga agaagaagta gcgactatac
    21541 ttgcaggttt cggtcacggc gaaggtcatg tctttaacct tggtcacggc attcatcagg
    21601 atgtgccgcc agaacatgct ggcgtgtttg tggaggcagt gcatcgcttg tctgaacagt
    21661 atcaccgcta aggagcgatt atggatctcg cgtcattacg cgctcaacaa attgaactgg
    21721 cttcttctgt gatccgcgag gatcgactcg ataaagatcc gccggatctg atcgccggag
    21781 ccgatgtcgg atttgagcag ggcggagaag tgacgcgagc ggcgatggtg ctgctgaaat
    21841 acccctcgct tgagctggtg gagtataaag tggcccgcat cgccaccacc atgccttaca
    21901 ttccaggttt tctttccttc cgcgaatatc ctgcgctgct ggcagcgtgg gagatgctgt
    21961 cgcaaaagcc ggatttagtg tttgtcgatg gtcacgggat ctcgcatcct cgccgtcttg
    22021 gcgtcgccag ccactttggt ttgctggtgg atgtgccgac cattggcgtg gcgaaaaaac
    22081 ggctctgcgg taaattcgaa ccgctctcca gcgaaccggg cgcgctggcc ccgctgatgg
    22141 ataaaggcga gcagctggcc tgggtctggc gcagcaaagc gcgctgtaac ccgttgttta
    22201 tcgctaccgg ccatcgggtc agcgtagaca gcgcgctggc gtgggtacaa cgctgcatga
    22261 aaggctatcg tctgccggag ccaacgcgct gggcggacgc ggtggcctcg gaacgtccgg
    22321 cgttcgtgcg ctatacagca aatcagccct aattcaggta aactgcggat aatttccgta
    22381 tttgagaact caacatgtta caaaacccaa ttcatctgcg tctggagcgc ctggaaagct
    22441 ggcagcacgt cacttttatg gcctgcttat gcgaacgcat gtaccctaat tacgccgtat
    22501 tctgtcagca aactggtttt ggtgatgggc agatttaccg ccgcattctc gatctcatct
    22561 gggaaacgct gaccgtcaaa gacgcaaaag taaatttcga cagccaactg gagaaatttg
    22621 aagaagcgat tccttcagcc gacgatttcg atctgtacgg cgtttatccg gcaatcgatg
    22681 cctgcgtggc gttaagtgaa ctggtccatt cgcgtttgag tggtgaaacg ctcgaacacg
    22741 cagtggaagt gagtaagacc tccatcacga ccgttgcgat gctggaaatg actcaggctg
    22801 gtcgcgaaat gagcgatgaa gagctcaaag aaaacccagc tgtagagcaa gaatgggaca
    22861 ttcagtggga aatattccga cttttagctg agtgcgaaga acgcgatatc gagctgataa
    22921 aaggccttag ggcagacctg cgtgaggcgg gcgagagcaa tattggtata atttttcagc
    22981 aataagacca gaaaacgtga tttaacgcct gatttgtcgt acctggagtc ttccctttcg
    23041 ccccccgtct ggtctacatt tggggggcga aaaaaagtgg ctatcggtgc gtgtatgcag
    23101 gagagtgcta ttctggcatt tccgtcgcac tcgatgctta gcaagcgata aacacattgt
    23161 aaggataact tatgaacaag actcaactga ttgatgtaat tgcagagaaa gcagaactgt
    23221 ccaaaaccca ggctaaagct gctctggagt ccactctggc tgcaattact gagtctctga
    23281 aagaaggcga tgctgtacaa ctggttggtt tcggtacctt caaagtgaac caccgcgctg
    23341 agcgtactgg ccgcaacccg cagaccggta aagaaatcaa aattgccgca gctaacgtac
    23401 cggcatttgt ttctggcaag gcactgaaag acgcagttaa gtaagattgc gtggcagtga
    23461 acagttttaa cgaaggggtg gtttcacccc ttttgtcttt ctggcgtcga tcattgatgc
    23521 tggcaggcgc tctgtttctc actgcctgta gtcacaactc ttcacttcct ccctttaccg
    23581 ccagtggatt tgctgaagac cagggcgcgg tacgcatctg gcgaaaagac agcggcgata
    23641 atgtgcatct gcttgccgtg tttagcccgt ggcgcagtgg cgataccacc acgcgagagt
    23701 atcgctggca gggcgataac cttacgctca tcaatatcaa tgtttacagc aaaccgccgg
    23761 tgaacattcg cgcgcgtttt gacgatcgcg gcgatctgag ctttatgcaa cgtgaatccg
    23821 atggggaaaa gcagcagctt tctaacgacc aaatcgattt ataccgttat cgtgctgatc
    23881 agatccgcca gattagcgat gctttacgtc aggggagagt cgtgctgcgc caggggcgct
    23941 ggcatgcgat ggaacagacc gtgaccacct gcgaaggaca aaccattaaa cctgatttag
    24001 attcgcaggc gatagcgcat atcgagcgcc gccagagccg ctcttctgtt gatgtcagcg
    24061 tggcatggct ggaagcgccc gaaggttcgc aattactgtt agtggcaaac tctgatttct
    24121 gtcgctggca acccaacgag aaaacgttct gatttaccag tggcccatac ccatatgacc
    24181 gccaccaccg cagccgccgt agcccatgcc cattccggct ccgcgcggaa tacccgcttc
    24241 agccatcgca atatcccgtt tcacccgtaa ctcatctaac gactgacgca aattctccat
    24301 ctctttggcg accgcgttaa ttttgctgct atccggtggg ttcgcggcta acagggcatt
    24361 gtattcataa cgcttcgtca ccagttgctg ttgcagtgcg ctgctttgag cgtaaaagtc
    24421 attatggatt ttctgccacg ctgtctgttg ttcgctggtc aaaggcgcgg cattttgctg
    24481 ccacataccg tgtccgccgt gagcaaatgc agatgtcgat cccatcgcca ttgctgaaag
    24541 cgccatcatc accagggcaa ttttcgtgtt ccgtttcatg gttaatcctc cagtggttgt
    24601 cttactttgg gtattgcatc tttcgtgcca acaacgaaac gctgatatga cgggtaatct
    24661 ggcatgataa acgagtaaaa atgactcgcc tgttgtgggt agcgagtcat ttttactcat
    24721 tgaaacttaa gtctttgtgt tacagcgcag ggtaagcgct gctaaaagat ggcatgattt
    24781 ctgctgtcaa aaagggatga acaggcaaag aagaagatac gttttatgca acgttcaaaa
    24841 gacaccttag ccaaatggtt aagcgcgatc ctccccgtgg tcattgttgg gctggtggga
    24901 ttgtttgcgg taacggtgat tcgtgattat gggcgggcaa gtgaggctga ccgtcaggca
    24961 ttactggaaa aaggtaatgt gctcattcgc gctctggagt caggaagccg cgtagggatg
    25021 gggatgcgaa tgcaccatgt gcagcaacag gcgcttctgg aagagatggc aggccagcct
    25081 ggggtgttgt ggtttgcggt caccgatgcg cagggcatca tcattcttca cagtaacccc
    25141 gaaatggtcg ggcgtgcact ctattctcct gatgaaatgc agcagttaaa gccagaggaa
    25201 aactcccgct ggagactgct tgaggatgca gaaaaaacgc ctgcgcttga ggtctatcgt
    25261 ttgttccagc caatgtcatt gccttggcgg catggtatgc acaatatgcc gcgctgtaac
    25321 ggcaaagctg tgccacaggc agaagcacaa caggctattt tcatcgccgt tgacgccagc
    25381 gatctggttg caacccagag tggggaaaag cgcaataccc tgattatcct cttcgccctg
    25441 gcgacggtct tgctggcaag cgtgttgtca ttcttctggt atcgccggta tctgcgctcg
    25501 cgccagcttc tacaagatga aatgaagcgc aaagagaagc tggtggcact ggggcatctg
    25561 gcggcaggcg ttgcccacga aatccgtaac ccactttcct cgattaaagg gctggcgaaa
    25621 tactttgccg aacgcgcgcc tgcaggggga gaagcgcatc aactggcgca agtgatggcg
    25681 aaagaggctg accgtttaaa ccgcgtggta agtgagttgc tggaactggt taagccaacg
    25741 catctggctt tgcaggcggt ggatctcaac actctgatta accactcatt acagctggta
    25801 agccaggatg caaacagccg ggagatccag ttacgcttta ccgccaacga catgttgccg
    25861 gaaattcagg ccgatccgga caggctgact caggtcctgt tgaatctcta tctcaatgct
    25921 attcaggcga ttggtcagca tggcgtgatt agcgtgacgg ccagcgaaag cggcgcaggc
    25981 gtgaaaatta gcgttactga tagcggtaag ggaattgcgg cggatcagct tgaagctatc
    26041 ttcaccccgt acttcaccac caaagccgac ggcaccgggt tggggctagc ggtcgtgcat
    26101 aatattgttg aacaacacgg tggtacaatt caggtcgcaa gccaggaggg agaaggcgca
    26161 acgttcaccc tctggcttcc ggtcaatatt acgcgtaagg acccacaagg atgacgcacg
    26221 ataatatcga tattctggtg gtagatgatg acattagcca ctgcactatt ttgcaggcat
    26281 tgctgcgcgg ctggggctat aacgtcgcgc tggcgaatag tgggcaacag gcgctggcgc
    26341 aggtgcggga acaggttttt gatcttgtgc tttgcgatgt gcgaatggca gagatggacg
    26401 gcatcgccac actaaaagaa atcaaagcgt taaacccggc aattccggta ctgattatga
    26461 ctgcgtactc cagcgttgag acggcagtag aggcgctgaa aaccggggcg ctggattatc
    26521 tcatcaagcc gctggatttc gataacctgc aggcgacgct ggaaaaggcg ctcgcacata
    26581 tgcacagtat tgacgctgaa acgcctgcgg tgtctgccag ccagttcggg atggtcggta
    26641 aaagtccggc gatgcaacac ctgctcagtg aaatcgccct cgtcgcgcca tcggaagcca
    26701 cggtgctgat ccacggcgat tccggcaccg gtaaagagct ggtcgccagg gcgattcacg
    26761 ccagtagcgc acggagtgaa aaaccactgg taacgctcaa ctgtgcggca ctcaacgaat
    26821 ccttgctgga atctgaattg ttcggtcacg aaaaaggggc gtttaccgga gccgacaaac
    26881 ggcgggaagg gcgctttgtt gaggcggacg gcggcacgct gtttctcgat gaaattggcg
    26941 atatctcgcc gatgatgcag gttcgtctgc tgcgtgcgat tcaggagcgc gaagttcagc
    27001 gtgtcggcag caaccagacc atctcggttg atgtccggct gattgcggcg actcatcgcg
    27061 atcttgccgc agaggtgaat gccggacgtt ttcgccagga tctctactat cgcctgaacg
    27121 tggtggcgat tgaagttccg tcgctgcgtc agcggcggga agatatccct ctgctggctg
    27181 gccattttct gcagcgcttt gccgagcgta atcgcaaggc ggtaaaaggt tttacgcccc
    27241 aggcgatgga tctgctgatt cactacgact ggccgggaaa tattcgcgag ctggaaaacg
    27301 cggtggaacg ggctgtggtg ctactgaccg gggaatatat ttccgaacgc gagctgccgc
    27361 tggcgattgc gagtacgccg atcccgctgg cgcaaagtca ggatattcag ccgctggtgg
    27421 aagtcgaaaa agaggtgatt ctggcggcac tggagaaaac gggcggcaac aaaaccgaag
    27481 ccgcccgtca gttagggatc acgcgtaaaa cgctattggc aaaactgtcg cgttagttct
    27541 gctcgcgttc gatagcgcgc cagccgatat ctttccggca gaagcagtcg tcccaatgga
    27601 tatcggtcat taaggcataa gcgcgtttct gcgcttctgc cacggtatga cccagcgcgg
    27661 tgacgcacag tacgcgcccg ccgctggtca ctacctgctc gtcatccgcc agtttcgtgc
    27721 ccgcgtggaa cactttgccg tcagacactt cttccagcgg caggccgtgg atcacatcgc
    27781 cggtgcggta atcgcccgga tatccacccg cagccatcac cacgccgaga gaagcgcgtt
    27841 catcccactc ggacgttttc tcgtccagct tgccttcaca ggccgccagg caaagctcaa
    27901 ccagatcgga cttcatgcgc agcataatcg gctgggtttc tggatcgcca aagcggcagt
    27961 taaattcaat aaccttcgga ttgccctgtt tgtcgatcat caggcccgcg tagagaaaac
    28021 cggtgtaggt gttgccttct gccgccatgc ctttcacggt tggccagatg attcgttcca
    28081 tcgtgcgctg atgaacctca tcggtcacta ctggcgcagg ggagtaagcg cccatcccgc
    28141 cggtgtttgg cccggtatct ttattaccta cgcgtttgtg atcctggctg gtggccatcg
    28201 gcagcacatg ctcgccgtcc accatcacga taaagctcgc ttcttcgccg tcgaggaact
    28261 cttcgataac aatgcgatga cccgcgtcgc caaaagcgtt gcccgccagc atatcgtgaa
    28321 cagccgcttc ggcttcttcc agcgtcatcg cgacgataac gcctttaccc gctgccagac
    28381 cgtcagcttt aatgacgatc ggcgcgccct tctcacgcag atacgccagc gcaggttcta
    28441 cctcggtgaa gttctggtat tccgccgtag ggatctgatg gcgggccagg aaatccttgg
    28501 taaacgcttt cgagccttcc agctgcgcgg cacctgcggt tgggccgaag attttcagcc
    28561 cggcggcgcg gaaggtatcc accacgcctt tcaccagcgg cgcttccggg ccgacgatgg
    28621 ttagatcgat cttttcgttt tgcgcgaaat ccagcagcgc cgggatatcg gttacgccga
    28681 tagcgacgtt ttgcagtgca ggctccagcg cagtgcctgc gttacccgga gcaacaaaaa
    28741 cagtttcaac cagcggcgac tgggccgctt tccaagccag cgcgtgctcg cgcccgccgt
    28801 taccaatcac taatactttc atctattgct ccattaatgg cggaagtggc gcatgtcggt
    28861 gaagagcatt gcaataccgt gctcgtcggc ggcggcaatc acttcgtcat cacggataga
    28921 accgccaggc tggattacgc aggtcacgcc cgcagcggcg gcggcatcaa taccgtcgcg
    28981 gaacgggaag aacgcgtcag aagccatcga ggaacctttc acttccagac cttcatcggc
    29041 agctttaata ccggcgattt ttgcggagta cacgcggctc atctggcccg cgccaatccc
    29101 gatggtcata ttgtttttgg catagacgat agcattggat ttcacgaact tcgccacttt
    29161 ccagcagaac agcgcatcac gcagttcctg ttcggtcggc tgacgtttgg tgacgacgcg
    29221 cagttcttct gcaccgacca tccccaggtc tcgatcctga accagcagac cgccgttcac
    29281 gcgtttgaaa tcaaggcccg gaacacgctc gccccactga ccgcaggtca gaacgcgtac
    29341 gttctgcttg gcggcggtga ttttcagggc ttcttcgctg gcggacggcg caataatcac
    29401 ttcgacgaac tgacgagaaa tgatggcctg tgcggtttcc gcatccagct cgcggttaaa
    29461 ggcgataatg ccgccgaatg cggaggtcgg gtcggttttg tacgcgcgat cgtaagcatc
    29521 aagaatggaa ttgccgatag ccacgccgca agggttggcg tgcttcacaa tcacacatgc
    29581 cggctcggcg aactctttca cgcactccag cgccgcatcg gtatcggcga tgttgttata
    29641 agagagggct ttaccctgaa cctgggttgc ggtagcaacg gaggcttctt tcacattctc
    29701 ttctatatag aaggcagcct gctggtggct gttctcgccg taacgcatat cctgcttctt
    29761 aatgaagttc aggttcagcg tgcgtgggaa gcgaccggcg gcttctttgc tttcaccgtg
    29821 gtaagccgga accatgctgc cgaagtagtt ggcaatcata ctgtcgtagg cggcggtatg
    29881 ttcaaaggct ttgatagcga gatcgaaacg ggttgcaagc agaagcgatc cttcgttggc
    29941 atccatctct ttaatgatgg cgtcgtagtc gctgctcttc accacgatgg cgacatcttt
    30001 atggttcttg gcggcggagc gcaccatcgt tgggccgccg atatcgatgt tctcaaccgc
    30061 atcttccagt gagcagcctt cacgggcaac ggtctgggcg aacggataca ggttaacaac
    30121 caccatatcg ataggctgga tctgatgttc ttccataatg gcatcgtcct ggccgcgacg
    30181 acccagaatg ccaccatgta ctttcggatg cagggtcttc acgcgtccat ccatcatctc
    30241 cgggaaaccg gtgtaatcgg aaacttcggt taccggcaga cctttttctg ctaacagacg
    30301 ggcagtgccc cctgtagaca gcagctccac accgcgtgcg gaaagtgcct gggcgaattc
    30361 gacgataccg gctttgtcag aaacactgag cagagcgcgg cggactggac gacgttgttg
    30421 catggtaaat cccctggatt tgactattac agagagcgtt agctgaattt ttcgcgaaaa
    30481 actcagctaa cgcccctaac ggggcatcct tatttttcgc ccgcattgta acgaaaacgt
    30541 ttgcgcaacg ctcgcgaatt tttctctttc aatggtgatc tcaattttga cagtgtttac
    30601 cgtgggaaaa atacagaaat tacattgatg attgtggata actctgtgcg taaaaaggta
    30661 taaagcgggg ttttgctggg gaatgcagca gtcagtcatt tttctgcaat ttttctattg
    30721 cggcctgcgg agaactccct ataatgcgcc tccatcgaca cggcggatgt gaatcacttc
    30781 acacaaacag ccggttcggt tgaagagaaa aatcctgaaa ttcagggttg actctgaaag
    30841 aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagccgcgtc gcaactgctc
    30901 tttaacaatt tatcagacaa tctgtgtggg cactcgaaga tacggattct taacgtcgca
    30961 agacgataaa tgaataccaa gtctcaagag tgaacacgta attcattacg aagtttaatt
    31021 ctttgagcat caaactttta aattgaagag tttgatcatg gctcagattg aacgctggcg
    31081 gcaggcctaa cacatgcaag tcgaacggta acaggaagaa gcttgcttct ttgctgacga
    31141 gtggcggacg ggtgagtaat g
//
LOCUS       DACBVI010000042        30879 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.042, whole genome shotgun sequence.
ACCESSION   DACBVI010000042 DACBVI010000000
VERSION     DACBVI010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.042"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            272..2944
                     /gene="rcsD"
                     /locus_tag="IU325_09300"
     CDS             272..2944
                     /gene="rcsD"
                     /locus_tag="IU325_09300"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HAP5809222.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTENVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            2961..3611
                     /gene="rcsB"
                     /locus_tag="IU325_09305"
     CDS             2961..3611
                     /gene="rcsB"
                     /locus_tag="IU325_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HAP5809223.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(3697..6546)
                     /gene="rcsC"
                     /locus_tag="IU325_09310"
     CDS             complement(3697..6546)
                     /gene="rcsC"
                     /locus_tag="IU325_09310"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HAP5809224.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRTDGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPLTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            6713..8539
                     /gene="atoS"
                     /locus_tag="IU325_09315"
     CDS             6713..8539
                     /gene="atoS"
                     /locus_tag="IU325_09315"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HAP5809225.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDLIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELNPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            8536..9921
                     /gene="atoC"
                     /locus_tag="IU325_09320"
     CDS             8536..9921
                     /gene="atoC"
                     /locus_tag="IU325_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HAP5809226.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEAKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            10117..10779
                     /gene="atoD"
                     /locus_tag="IU325_09325"
     CDS             10117..10779
                     /gene="atoD"
                     /locus_tag="IU325_09325"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HAP5809227.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            10779..11429
                     /locus_tag="IU325_09330"
     CDS             10779..11429
                     /locus_tag="IU325_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HAP5809228.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            11426..12748
                     /locus_tag="IU325_09335"
     CDS             11426..12748
                     /locus_tag="IU325_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HAP5809229.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSDFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            12779..13963
                     /locus_tag="IU325_09340"
     CDS             12779..13963
                     /locus_tag="IU325_09340"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HAP5809230.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERARID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTTEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPAFMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(14037..14813)
                     /locus_tag="IU325_09345"
     CDS             complement(14037..14813)
                     /locus_tag="IU325_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2135 domain-containing protein"
                     /protein_id="HAP5809231.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGAGAIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNSVLKNSGALDMDVTTGYGPEIFAMPAPIHGRYQVYINYYGGRSETE
                     LTTAQLTLITEEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(14818..16467)
                     /locus_tag="IU325_09350"
     CDS             complement(14818..16467)
                     /locus_tag="IU325_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2300 domain-containing protein"
                     /protein_id="HAP5809232.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDTLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GSPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFANGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     SCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(16468..20862)
                     /locus_tag="IU325_09355"
     CDS             complement(16468..20862)
                     /locus_tag="IU325_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HAP5809233.1"
                     /translation="MRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRI
                     VVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKP
                     SRYVQNSQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPL
                     GKQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEIL
                     WTDGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLY
                     IFTDRPLYRAGDRVDVKVMGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTINVTLDA
                     RNGGQGSFRLPENAVAGGYELRLAYRKQVYSSSFRVANYIKPHFEIGLALDKKDFKTG
                     EAVSGKLQLLYPDGEPVKDASVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSDAVSDDK
                     GNVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYSLSTSAQYSNSGE
                     SVVFRYAALESSKQVPVKYEWLRLEDRTSHSGDLPSGGKSFTVNFDKPGNYNLTLRDK
                     DGLILAGLSHAVSGKGSTSHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLER
                     DRVEQQSLLSHPANWLTLQRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAG
                     IKVAVPQLDIRVKTDKTHYQPGELVNVELTSLLKGKPVSAQLTVGVVDEMIYALQPEI
                     APNIGKFFYPLGRNNVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREE
                     VDTAAWMPSLTTDKQGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLY
                     MKWSMPTVYRVGDKPAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQ
                     NIQQSGLLSAELQQNGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALILPEQASN
                     IRLQSSETPQEIFRNNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQ
                     MIQDNRLRLMQLAGPGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQH
                     MLDSYAEQADNMPLLHRALVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTR
                     DINDSLILDTPESPLADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAH
                     TVVLLNSGGDATQAAAILSGLTAEQSTIERALAMNWLAKYMATMPSVVLPAPAGAWAK
                     HKLTGGGEDWRWVGQGVPDILSFGDELSPQNVQVRWREPAKTAQQSNIPMTVERQLYR
                     LIPGEEEMSFTLQPVTSNEIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERT
                     TWGISVNKPNAAKQQGQLLEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVL
                     PPARYVRSYAPAQQSVAAGSEWTGMQVK"
     gene            complement(21006..21629)
                     /locus_tag="IU325_09360"
     CDS             complement(21006..21629)
                     /locus_tag="IU325_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HAP5809234.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFSANEALKVHDSKWLKSNGIASQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(21626..23314)
                     /locus_tag="IU325_09365"
     CDS             complement(21626..23314)
                     /locus_tag="IU325_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 family protein"
                     /protein_id="HAP5809235.1"
                     /translation="MSGEKKAKGWRFYGLVGLGAIVLLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKLNSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERTAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMSNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVNQTQQGEAQIWRREVSSRYGQYPKTQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPDAAWQWLPITWQPL"
     gene            complement(23463..26090)
                     /gene="gyrA"
                     /locus_tag="IU325_09370"
     CDS             complement(23463..26090)
                     /gene="gyrA"
                     /locus_tag="IU325_09370"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HAP5809236.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            26237..26959
                     /locus_tag="IU325_09375"
     CDS             26237..26959
                     /locus_tag="IU325_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HAP5809237.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVEYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(27020..30748)
                     /gene="yfaL"
                     /locus_tag="IU325_09380"
     CDS             complement(27020..30748)
                     /gene="yfaL"
                     /locus_tag="IU325_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HAP5809238.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAVIDSCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMNNNASGGAVFLQQGAEFSLSPENETGMTLFAN
                     NTVSGEYNNGGAIFAKENSTLNLTDVIFSDNVAGGYGGAIYSSGTNDTGVVDLSVTNA
                     VFRNNIANDGKGGAIYTINNDVYLSEVIFDNNQAYTSTSYSDGDGGAIDVTDNNSNIT
                     HPSGFTIINNTAFTNNSAEGYGGAIYTNSVTAPYLIDISVDDSYSQNNGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGVITKTGSG
                     DLVLNANNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQN
                     QAELNVGSTQQTFVHALTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDDGAVLTLEGDAADLAALQDDPQSIVLNGGVLDLSDFATWQS
                     GTSYNDGLEVSGSGGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVIDAGDGLVSLANN
                     NSYLGTTQIASGTLVVSDNSQLGNTDENRQLIFTDSQQQSEMEITADVDTRSEAAGHG
                     RDIEMRADGEVAVDAGVDTQWGGLMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADIFPYASSLWVGDGATFKTGADQDIQSIDVTSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSASDQLVLNGNTAGNTTVVIN
                     PITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEATPPSPPDPDPTPDPTPDPEPTPAYQPVLNAKVGGYFNNLRAANQAFVMERHDHA
                     GGDGQTLNLRIIGGDYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGADGEWMLGAVGGY
                     SDNQGDSRSNMTGTRADNQNHGYAVGLTSSWFQHGNQKQGAWLDSWLQYAWFNNDVSE
                     HEDGVDHYHSSGIIASLEAGYQWLPGHGVVIEPQAQVIYQGVQQDDFTAANHARVSQS
                     QGDDIQTRLGLHSEWRTAVHVIPSLDLNYYHDPHATEIEEDGSTISDDAVKQRGEIKV
                     GVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
BASE COUNT         7421 a         8305 c         7612 g         7541 t
ORIGIN      
        1 gaaaaactat aacgcttgtt aactatttca caaataatta acattagcat aatttccagc
       61 aatcttcgtt tatttgcaat taatttcgtt gggctttttg taggttattt gtacagcaaa
      121 atggcgcttg tacatctatt tcccccaatg caggatgata aatatcacgg gagaatagag
      181 aatcatcaat caggtaagag tctggaattt cacactgtac cctttatact gccctatcac
      241 ttcgcgaagt tttaacaggt cataaacacg aatgcgtcag aaagagacaa cggccacgac
      301 ccgcttttca ctcctaccgg ggagcattac ccgcttcttt ttactgttga tcattgtgtt
      361 actggtgacg atgggagtaa tggtacaaag cgccgttaac gcctggctga aagataaaag
      421 ttaccagatt gtcgacatta cccacgcaat ccaaaagcgc gtcgatacct ggcgttacgt
      481 gacctggcag atctacgaca acattgccgc gacgacctcc ccctcctccg gcgaaggttt
      541 acaagagacg cgcctgaaac aggatgtcta ctatctggaa aaaccgcgcc gcaaaacgga
      601 agcgttaatc tttggctctc acgacaactc aacgcttgag atgactcaac ggatgtccac
      661 ttatctggat acattgtggg gcgcagaaaa tgtaccgtgg tcgatgtatt acctgaatgg
      721 tcaggataac agcctggtgc tgatctcaac cctgcccctt aaagatctca cctccggatt
      781 taaagaatcg accgtcagcg acattgttga ttcacgtcgt gcagagatgt tgcaacaggc
      841 caacgccctc gatgaacgcg aaagcttttc taacatgcgc cgcctagcct ggcagaacgg
      901 tcattacttt accttgcgta ctacctttaa ccagccggga catctggcaa cggtcgtggc
      961 ttttgatctg ccgattaatg atttgatccc accgggcatg ccgctggaca gtttccgcct
     1021 tgagccagac gcgacggcaa cgggaaacaa tgataatgag aaagaaggga cggatagcgt
     1081 cagtatccac tttaacagta cgaagattga aatctcctcg gcactcaact ctaccgatat
     1141 gcgcctggtc tggcaggttc cttatggcac cttactgctg gatacgttgc aaaacattct
     1201 gctgccactg ctgctgaata tcggtttgct ggcgctggcg ttatttggct ataccacatt
     1261 ccgccatttc tccagccgca gtacagaaaa cgtccccagc acggcggtca ataacgaatt
     1321 gcgcatttta cgtgcaatca atgaagagat agtctcactg ctgccgcttg gcctgctggt
     1381 tcacgatcag gaatcgaacc gcactgtcat aagtaacaaa attgccgatc atttgctgcc
     1441 gcatttgaat ctgcaaaaca tcaccaccat ggcggaacag catcagggga ttattcaggc
     1501 gacgatcaat aacgagctgt atgagatccg catgttccgc agccaggtcg cgccgcgcac
     1561 acaaattttc attattcgcg atcaggatcg cgaagtgctg gtaaacaaga aactcaagca
     1621 ggcgcagcgt ctgtatgaga aaaaccagca ggggcggatg acctttatga aaaacattgg
     1681 cgatgcgctg aaagaacctg cacagtccct ggcggagagc gcggctaaac tcaacgcccc
     1741 ggaaagcaaa caactggcga atcaggcaga tgtgctggtg cggctggtcg atgaaataca
     1801 gttagcgaac atgcttgcgg atgatagctg gaaaagtgag acggtgctgt tctccgtgca
     1861 ggatttaatt gatgaagttg tgccttcagt gttgcctgcc atcaagcgta aaggtctgca
     1921 actgctgatt aacaatcatc tgaaagcaca cgatatgcgc cgcggcgatc gcgatgcctt
     1981 acgacgcatt ttgctgctac tgatgcaata tgccgtgacc tcaacgcaat tgggaaaaat
     2041 cacccttgag gttgatcagg atgagtcctc cgaagaccgc ctgacgttcc gcattctgga
     2101 caccggagaa ggcgtaagca ttcatgaaat ggataatttg cacttcccgt ttatcaacca
     2161 gacccaaaac gatcgctatg gcaaggcgga cccgctggca ttctggctga gcgatcaact
     2221 ggcacgtaaa ctgggcggtc atttaaacat taaaacgcgg gatgggcttg gtacacgcta
     2281 ctctgtgcat atcaaaatgc tcgcagctga cccggaagtt gaagaggaag aagagcgttt
     2341 actggatgat gtctgcgtaa tggtggatgt tacttcggca gaaattcgga atattgtcac
     2401 tcgccagtta gaaaattggg gtgcaacctg tatcacaccc gatgaaagat taattagtca
     2461 agattatgat atctttttaa cggataatcc gtctaatctt actgcctctg gcttgctttt
     2521 aagcgatgat gagtctggcg tacgggaaat tgggcctggt caattgtgcg tcaacttcaa
     2581 tatgagcaac gctatgcagg aagcggtctt acaattaatt gaagtgcaac tggcgcagga
     2641 agaggtgaca gaatcgcctc tgggcggaga tgaaaatgcg caactccatg ccagcggcta
     2701 ttatgcgctc tttgtagaca cagtaccgga tgatgttaag aggctgtata ctgaagcagc
     2761 aaccagtgac tttgctgcgt tagcccaaac ggctcatcgt cttaaaggcg tgtttgccat
     2821 gctaaatctg gtacccggca agcagttatg tgaaacgctg gaacatctga ttcgtgaaaa
     2881 ggatgttcca ggaatagaaa aatacatcag cgacattgac agttatgtca agagcttgct
     2941 gtagcaaggt agcctattac atgaacaata tgaacgtaat tattgccgat gaccatccga
     3001 tagtcttgtt cggtattcgc aaatcacttg agcaaattga gtgggtgaat gttgtcggcg
     3061 aatttgaaga ctctacagca ctgatcaaca acctgccgaa actggatgcg catgtgttga
     3121 ttaccgatct ctccatgcct ggcgataagt acggcgatgg cattacctta atcaagtaca
     3181 tcaagcgcca tttcccaagc ctgtcgatca tcgttctgac catgaacaac aacccggcga
     3241 ttcttagtgc ggtattggat ctggatatcg aagggatcgt gctgaaacaa ggtgcaccga
     3301 ccgatctgcc gaaagctctc gccgcgctgc agaaagggaa gaaatttacc ccggaaagcg
     3361 tttctcgcct gttggaaaaa atcagtgctg gtggttacgg tgacaagcgt ctctcgccaa
     3421 aagagagtga agttctgcgc ctgtttgcgg aaggcttcct ggtgaccgag atcgctaaaa
     3481 agctgaaccg cagtattaaa accatcagta gccagaagaa atctgcgatg atgaagctgg
     3541 gtgtcgagaa cgatattgct ctgctgaatt atctctcttc tgtgacctta agtccggcag
     3601 ataaagacta atcatctgta ggccagataa gacgcgtcag cgtcgcatct ggcatttgca
     3661 ctgaatgcca gatgcggcgt aaacgcctta tccgccctac gaatcccgcg atttcctgac
     3721 cctctcggca tataccgtta gcgtctgttt tatcacatcc agcgttaccg gcttcgacag
     3781 gcagctgtcc ataccagact ccagacaccg ctgcttctct tcagccaacg cattagcagt
     3841 tactccgatt accggcaacg tcagtcccaa ctgacgaatg cgttgcgtca agcggtaacc
     3901 atccatattt ggcatgttga cgtcgctaag cacgatatca atatgattct tgctaagtac
     3961 attaagcgca tcgacgccat cattcgcggt tttacattga tagcccaacg atcccaactg
     4021 atctgccagc agacgccggt taatcggatg atcatccacg accagaatca tcatatcgtc
     4081 gttatcgctg accgctttgt ccgttaacgg cagagcgtta gcaggatcgt cgctctccat
     4141 ctcgatcaaa taaatacgcg ccaacaatgc cggtagctca tgcggggcag ccacactgtg
     4201 tacccactcc cctggcgctt tctccagcgg aataccaata tggcgacgac agaaggtcac
     4261 taccgctcta ccctgccatt ttttactcac tacctcgtca gtaattaaca catcttcggg
     4321 agtcggttcc tgcccttcgt atgttgtaac gacgatgccg ctgcgctgca aactggtttc
     4381 caggaactga cagagcgacg cattgcggac cgccagccag cagcgtttac cactcaaccc
     4441 ttccacgcct tttttctgcg ggtactgagc gccgtacaac ggaatacgca cggtaaactg
     4501 gctacccatt cccggttctg aatctaccga gatatcgccg tccatcatgc tgatcagttt
     4561 ttcacaaatc gccagaccca gaccggtccc ctggaagtta cgctgtacgc ccgttccgac
     4621 ctggaagaag ggatcaaaca agcgcaccac ttctttcgcc ggaatcccca cgccggtatc
     4681 gcgaacacgg atagagagat aatcgccatc agtgcgaaca tgcaaaacta tacagccggt
     4741 atcggtgaat tttatggcgt tactcaacag gttggagatg acctgctgta aacgcatcgg
     4801 gtcgccattt aaggccactg gcacatccgg ttcaataaag cagtacaagc ctaactgctt
     4861 gcgtaccacc agcggtaaat agttggcggt gatgtggttc atcacttcac gcggtgaaaa
     4921 ctcacgcggt tcgatcttca actgttccga ttcaatcttc gagaaatcga gaatatcgct
     4981 gataattttc aacaacaggc tggaagagtt gttcattgcc gtcaccagac gatcgacgcc
     5041 tttcggtaac tctttggttt gcaacagatc caggttaccg ataatgccat acagcggcgt
     5101 tcgcagctca tgactgacgg tggcaaggaa catcgatttt gactggctcg cctgttccgc
     5161 tgcttgtgcc atctcctgca acgactcttc catcttcacg cgcgaagaaa catccaccag
     5221 cacacaaatg gccacgtttt cattacgata gcgcgaatgg acgaagctga tttgcagatt
     5281 ggtattgttg ctggtcagga catcaacaaa attgacctgc tgcccacaga taatttgtgt
     5341 cagccgttgg cgatcctcat gcgtaagcat attgagatag gtatgcgcca gttcgttact
     5401 taaaatattg acgccatcag cggtacgcaa aatgcagata cccactggcg cggaggcgac
     5461 aatcttgcga ttaaactgct catgttcttc cagtcgcagg gcgtcgcttt ccgccggaat
     5521 gaaaatacgt cgctcgtaca tccgcgcgag agtaaacaat gcagctccgg caagcacatt
     5581 cagcaaaatt gcgttaagga tcaacatgcg aatgcgttcc agcaccttat caaccggcac
     5641 cgaatacacg atgcttagcg atgagggtgg cagatttttc ttcagcacca gctcccggaa
     5701 cccttccgta tagccaaacc aggagcgttc ctgcatccag cgaggatcgc ccttaatttt
     5761 actttctggt ccggtaagcg aaatcagggt atgaccattt tcatcaagaa tggtaacccc
     5821 catcggcaac gtaccaggta agaaaaaatt ctccatccgg atagtctgct cgacacccaa
     5881 aagcgcctgc aaccggttcg ccagataaac tggcgtcaac gcgtaaaaat acccgacgcc
     5941 agggcgcgga ccttcgctaa tccagtagag gttactgcca ctatcatctt gtggtgcatt
     6001 tcgatattta ttaatgcgtt catgcaacgc tttcaacgcg gtatcgcgtt ccactggcat
     6061 atcacgcaga ccgaaattgg ccatgcagag gttatcgctg ccgattaaaa atacccggtt
     6121 gagatcgtaa gccgcagaga aattatcgcg ccagtagcgc ataaaccacg ccaatgactc
     6181 cagagagcct cgccaggtgt tactcattgc ggaacaatcg gagtcggcaa acagcggttc
     6241 aaacgcaggc acatccgcct gcgtttctcg tccacgcggg gaaagcacac cgttttctgc
     6301 cgataagcga ttttcagcga tgtacttcag ctctttcatc acatcagagg tacgttgaat
     6361 aaagcgctga gcctgatcgg aactcagatt aaattcctga cgaatttccg attctcgctg
     6421 atgtaacgca ttaacgatgt aaaaaacgga tgaaaaagca atcaacagcc agagcactaa
     6481 cgccaatgct ctgaacatgt agcgcgaggc tttcagggtt gtacgaaaag aagcaaggta
     6541 tttcaaaggg gcgaagctcc gcctcaggtg accgatggag tgtggttaag gtagcggtaa
     6601 aagcgtgtta ccgcaatgtt ctctcttctc tggaatatga tacaccgccg agaaatcatc
     6661 accttaacct ctgataatcg tcatataccg gacaagacta gtggatttca gcatgcatta
     6721 tatgaagtgg atttatccac gccgcttacg caatcaaatg attctgatgg caatcctgat
     6781 ggtcattgtc ccaacgctta ctattggtta tatcgtagaa acggaaggac gttcagcagt
     6841 cttatctgaa aaagagaaaa aactttctgc cgtggtcaac ctgcttaatc aggcactagg
     6901 cgatcgctat gatctctaca tcgacttacc acgtgaggag cgtatccgcg cattaaatgc
     6961 agaacttgcc cccattaccg aaaatatcac tcacgccttc cctggcatcg gcgctggtta
     7021 ttacaacaaa acgctggatg cgataatcac ctacgcgcct tcagcgctat atcagaataa
     7081 tgtcggcgtt accattgccg cagatcaccc tggtcgcgaa gtcatgcgta caaatacccc
     7141 tttggtttat tcaggcaggc aggtgcgcgg cgatattttg aattcaatga tccccattga
     7201 gcgtaatggt gaaatcctcg gctatatctg ggccaatgaa ttaaccgaag atattcgccg
     7261 ccaggcctgg aaaatggatg tgaggattat cattgtgctc accgccggtt tgctgataag
     7321 cctgctgttg attgtccttt tctcccgtcg cctgagcgcc aatattgata tcatcaccga
     7381 tggcctctcg actctggcac aaaatattcc cactcgatta ccacaattgc ccggtgaaat
     7441 ggggcaaatc agtcagagtg ttaacaacct cgcccaggca ctgcgtgaaa cgcggacact
     7501 taacgatctg attattgaaa acgctgccga tggcgtcatt gccattgacc gccagggtga
     7561 tgtaaccacc atgaacccgg cagcagaagt catcactggc tatcaacgtc atgaactggt
     7621 agggcagcct tactccatgt tgttcgacaa tactcagttc tacagtccag tactggatac
     7681 gctggaacat ggcaccgaac atgtagcgct ggagatcagt tttccaggcc gtgaccgcac
     7741 cattgaactc agtgtcacta ccagtcgtat tcataacacg catggtgaaa tgataggtgc
     7801 tttggtgatt ttctctgatt taactgcccg caaagaaacc cagcgccgca tggcgcaagc
     7861 agaacgcctc gccacactgg gtgagctgat ggctggcgtc gcgcatgaag tacgtaatcc
     7921 gctaacggct attcgtggtt atgtacagat cttgcgccaa caaaccagcg acctaataca
     7981 tcaggaatat ctgtccgtag tactcaaaga aatcgattca attaacaaag ttattcagca
     8041 attgctcgaa ttttcacgtc cacgccacag tcaatggcaa caagtcagtc tcaatgcatt
     8101 ggttgaagaa actctggtac tggtacaaac cgccggcgta caagcgcggg tcgacttcat
     8161 aagcgaactg gataatgaat taaacccgat taacgccgat cgtgaactgc tcaaacaggt
     8221 actactgaat atcctgatca atgccgtcca ggctatcagc gcacgaggga aaattcgcat
     8281 tcaaacctgg caatacagcg actcacaaca ggccatttcg atagaggaca acggctgtgg
     8341 cattgatctc tcgctgcaaa aaaagatctt cgatcccttt ttcaccacca aagcctctgg
     8401 aaccgggctt ggtctggcgt taagtcaacg catcattaat gcccatcagg gtgatattcg
     8461 cgtcgccagt ttgccgggct acggcgcaac cttcacgctt attttaccga tcaatccgca
     8521 gggaaatcag actgtatgac tgctattaat cgcatcctta ttgtggatga tgaagataat
     8581 gttcgccgta tgctgagcac cgcttttgca ctacaaggat tcgaaacaca ttgtgcgaac
     8641 aacggacgca cagcattaca cctgtttgcc gatattcacc ctgacgtggt gttgatggat
     8701 atccgcatgc cagagatgga cggcatcaag gcactcaagg agatgcgcag ccatgagaca
     8761 cggacccccg ttattctgat gacggcctat gctgaagtgg aaaccgccgt cgaagcgcta
     8821 cgctgcgggg ccttcgacta tgttatcaaa ccgtttgatc tcgatgagtt gaatttaatc
     8881 gttcagcgcg ctttacaact ccagtcaatg aaaaaagaga tccgtcatct gcaccaggca
     8941 ctgagcacca gctggcaatg ggggcacatt ctcaccaaca gccctgcgat gatggatatc
     9001 tgcaaagaca ccgccaaaat tgccctttct caggccagcg tcttgattag cggtgaaagc
     9061 ggcaccggga aagagttgat tgccagagcg attcactaca attcgcggcg ggcaaagggg
     9121 ccgttcatta aagtcaactg cgcggcgctg ccggaatcgt tgctcgaaag tgaactgttt
     9181 ggtcatgaaa aaggtgcatt tactggtgca caaaccttgc gtcagggatt atttgaacgt
     9241 gccaacgaag gtactctgct cctcgacgaa attggcgaaa tgccgctcgt actgcaagcc
     9301 aaattactac gcattctgca ggaacgggaa tttgaacgga ttggcggcca tcagaccata
     9361 aaagttgata tccgcatcat tgctgccacc aaccgtgact tgcaggcaat ggtaaaagaa
     9421 ggcaccttcc gtgaagatct cttttatcgc cttaacgtta ttcatttaat actgccgcct
     9481 ctgcgcgatc gccgggaaga tatttccctg ttagctaatc actttttgca aaaattcagt
     9541 agtgagaatc agcgcgatat tatcgacatc gatccgatgg caatgtcact gcttaccgcc
     9601 tggtcatggc cgggaaatat tcgagagctt tccaacgtta ttgaacgcgc cgtcgtgatg
     9661 aactcaggcc cgatcatttt ctctgaggat cttccgccgc agattcgtca gccagtctgt
     9721 aatgctggtg aggcaaaaac agcccctgtc ggtgagcgta atttaaaaga ggaaattaaa
     9781 cgcgtcgaaa aacgcatcat tatggaagtg ctggaacaac aagaaggaaa ccgaacccgc
     9841 actgctttaa tgctgggcat cagtcgccgt gcattgatgt ataaactcca ggaatacggt
     9901 atcgatccgg cggatgtata acgccaaaac ttgctatgca gatatttgca cagtgcgcaa
     9961 ttttctgcat agccgctcat tctccttata aatcctcatc caatttatcc cttcatattc
    10021 aattagttaa ataacaaaac ccaataatct cattctggca ctccccttgc tattccctga
    10081 ctgtacccac aacggtgtat gcaagaggga taaaaaatga aaacaaaatt gatgacatta
    10141 caagatgcca ccggcttctt tcgtgacggc atgaccatca tggtgggcgg atttatgggg
    10201 attggcactc catcccgcct ggttgaagca ttactggaat ctggtgttcg cgacctgaca
    10261 ttgatagcca atgataccgc gtttgttgat actggcatcg gtccgctcat cgtcaatggt
    10321 cgagtccgca aagtgattgc ttcacatatc ggcaccaacc cggaaacagg tcggcgcatg
    10381 atatctggtg agatggacgt cgttctggtg ccgcaaggta cgctaatcga gcaaattcgc
    10441 tgtggtggag ctggacttgg tggttttctc accccaacgg gtgtcggcac cgtcgtagag
    10501 gaaggcaaac agacactgac actcgacggt aaaacctggc tgctcgaacg cccactgcgc
    10561 gccgacctgg cgctaattcg cgctcatcgt tgcgacacac ttggcaacct gacctatcaa
    10621 cttagcgccc gcaactttaa ccccctgata gcccttgcgg ctgatatcac actggtagag
    10681 ccagatgaac tggtcgaaac cggcgagctg caacctgacc atattgtcac ccctggtgcc
    10741 gttatcgacc acatcatcgt ttcacaggag agcaaataat ggatgcgaaa caacgtattg
    10801 cgcgccgtgt ggcgcaagag cttcgtgatg gtgacatcgt taacttaggg atcggtttac
    10861 ccacaatggt cgccaattat ttaccggagg gtattcatat cactctgcaa tcggaaaacg
    10921 gcttcctcgg tttaggcccg gtcacgacag cgcatccaga tctggtgaac gctggcggac
    10981 aaccgtgcgg tgttttaccc ggtgcagcca tgtttgatag cgccatgtca tttgcgctaa
    11041 tccgcggcgg tcatattgat gcctgcgtgc tcggcggttt gcaagtagac gaagaagcaa
    11101 acctcgcgaa ctgggtagtg cctgggaaaa tggtgcccgg tatgggtggc gcgatggatc
    11161 tggtgaccgg gtcgcgcaaa gtgatcatcg ccatggaaca ttgtgccaaa gatggttcag
    11221 caaaaatttt gcgccgctgc accatgccac tcactgcgca acatgcggtg catatgctgg
    11281 ttactgaact ggctgtcttt cgttttattg acggcaaaat gtggctcacc gaaattgccg
    11341 acgggtgtga tttagccacc gtgcgtgcca aaacagaagc tcggtttgaa gtcgccgccg
    11401 atctgaatac gcaacggggt gatttatgat tggtcgcata tcgcgtttta tgacgcgttt
    11461 tgtcagccgg tggcttcccg atccactgat ctttgccatg ttgctgacat tgctaacttt
    11521 cgtgatcgcg ctttggttaa caccacaaac gccgatcagc atggtgaaaa tgtggggtga
    11581 cggtttctgg aacttgctgg cgtttggtat gcagatggcg cttatcatcg ttaccggtca
    11641 tgcccttgcc agctctgctc cggtaaaaag tttgctgcgt actgccgcct ccgccgcaaa
    11701 gacgcccgta cagggcgtca tgctggttac tttcttcggt tcagtcgctt gtgtcatcaa
    11761 ctggggattt ggtttggttg tcggcgcaat gtttgcccgt gaagtcgccc gccgagtacc
    11821 cggttctgat tatccgttgc tcattgcctg cgcctacatt ggttttctca cctggggtgg
    11881 cggtttctct ggctcaatgc ctctgttggc tgcaacaccg ggcaacccgg ttgagcatat
    11941 cgccgggctg atcccggtgg gcgatactct gttcagtgat tttaatattt tcatcactgt
    12001 ggcgttgatt gtggtgatgc catttatcac ccgcatgatg atgccaaaac cgtctgacgt
    12061 ggtgagtatc gatccgaaac tactcatgga agaggctgat ttccaaaagc agctaccgaa
    12121 agatgcccca ccatccgagc gactggaaga aagccgcatt ctgacgttga tcatcggcgc
    12181 actcggtatc gcttaccttg cgatgtactt cagcgaacat ggcttcaaca tcaccatcaa
    12241 taccgtcaac ctgatgttta tgattgcggg tctgctgcta cataaaacgc caatggctta
    12301 tatgcgtgct atcagcgcgg cagcacgcag tactgccggt attttggtgc aattcccctt
    12361 ctacgctggg atccaactga tgatggagca ctccggtctg ggcggactca ttaccgaatt
    12421 cttcatcaat gttgcgaaca aagacacctt cccggtaatg acctttttta gttctgcact
    12481 gattaacttc gccgttccgt ctggcggcgg tcactgggtt attcagggac ctttcgtgat
    12541 acccgcagcc caggcgctgg gcgctgatct cggtaaatcg gtaatggcga tcgcctacgg
    12601 cgagcaatgg atgaacatgg cacaaccatt ctgggcgctg ccagcactgg caatcgccgg
    12661 actcggtgtc cgcgacatca tgggctattg catcactgcc ctgctcttct ccggcgtcat
    12721 tttcgtcatt ggtttaacgc tgttctgacg gcaaccctac agacagaagg aataaaaaat
    12781 gaaaaattgt gtcatcgtca gtgcggtacg tactgctatc ggtagtttta acggctcact
    12841 cgcttccacc agcgccatcg acctgggggc gacagtaatt aaagccgcca ttgaacgtgc
    12901 aagaatcgat tcactacaca ttgatgaagt gattatgggt aacgtgttgc aagccgggtt
    12961 ggggcaaaat ccggcacgtc aggcactgtt aaaaagcggg ctggcagaaa cggtgtgcgg
    13021 attcacggtc aataaagtgt gtggttcggg tcttaaaagt gtggcgcttg ccgcccaggc
    13081 aattcaggca ggtcaggcgc aaagcattgt ggcggggggt atggaaaata tgagtttagc
    13141 gccctactta ctcgatgcaa aagcacgctc tggttatcgt cttggagacg gacaggttta
    13201 tgacgtaatc ctgcgcgatg gtctgatgtg cgccacccat ggttatcata tggggattac
    13261 cgccgagaac gtggccaaag agtacggaat tacccgagaa atgcaggatg aactggcgct
    13321 acattcacag cgtaaagcgg cagccgcaat tgagtccggt gcttttacag ccgaaatcgt
    13381 cccggtaaat gttgtcactc ggaagaaaac cttcgtcttc agtcaagacg aattcccgaa
    13441 agcggattct acgactgaag cgttaggcgc attgcgcccg gccttcgata aagcaggaac
    13501 agtcaccgcc gggaacgcgt ctggtattaa cgacggtgct gccgctctgg tgattatgga
    13561 agaatctgcg gcgctggcag caggccttac ccccctggct cgcattaaaa gttatgccag
    13621 cggtggcgtg ccccccgcat ttatgggtat ggggccagta cctgccacgc aaaaagcgtt
    13681 acaactggcg gggctgcaac tggcggatat tgatctcatt gaggctaatg aagcatttgc
    13741 tgcacagttc cttgccgttg ggaaaaccct gggctttgat tctgagaaag tgaatgtcaa
    13801 cggcggggcc atcgcgctcg ggcatcctat cggtgccagt ggtgctcgta ttctggtcac
    13861 actattacat gccatgcagg cacgcgataa aacgctgggg ctggcaacac tgtgcattgg
    13921 tggcggccag ggaattgcga tggtgattga acggttgaat taatcaataa aaacacccga
    13981 tagcgaaagt tatcgggcgt tttcttgaac atcgacggcg aaggtaaccc cattaatcac
    14041 cagtcaaaac ttttcaccag cgtcagctcg ccagcattac gcatcggtac aataaatgtt
    14101 tcctgtttct cattgaccga tccttcctcg gtgatcagcg tcagttgggc ggtcgtcaat
    14161 tccgtttcgc tgcgtccgcc atagtagttg atatacacct gataacgtcc gtgaatcggc
    14221 gctggcatgg cgaaaatctc ggggccgtac cccgtcgtga catccatatc cagtgcacca
    14281 ctgtttttca gcacgctgtt accgtaccag gcgtgttcgc catcaggtgt aacaacgtga
    14341 aggtcgaggt cggtattgtc cgtgtcccag gagagaacca gccgtaaacg cgcgcggatc
    14401 gctccagcgc ccggcgttga gtaaaactgc attttttgtc ggctttgccc atccgggctg
    14461 atgacctgca cgctgttgct gccttcagta aaaatataag ggcgtgcaaa tgaaccatcg
    14521 gattcaattc tctgcggcat actggcaccg ttgaccacca gccggccctg ctgaacttta
    14581 cccgccgcag cataattctt tattttgccg cggatctgcg ctgagatatt ttgatcatct
    14641 gccatgttga cggacgacgc cgggtaattg atcgattgtg aaaaactggc atcttcgccc
    14701 tctgccggat gccagccaga gagcggtgca tcaatctcga cttcctgcac accttcactg
    14761 tgagcgacag gcgaaagcgc caccagtaac aacggaagaa aaatctttcg catagcatta
    14821 gtccattaat aattgtcggg tcagcgtttc gatatagttc tcatcaagcc cggtgggata
    14881 accatcaaag gccaggtgca gatactcatg ggttaaatcg agccgatcct gaagcgtaaa
    14941 gaagttacga ataaacagcc gtttctgctg ccgatcggta taggggaaac ctgagactaa
    15001 acgacagacc gcaaacacgt ctggttcgtt gtaccccgtc tcgccttgta atatacgccg
    15061 ccactgcggc atttttttcg ccagccaggc tttcgctttg ggtaataact ggcagcttga
    15121 gcgtggcgct ccccagcggc taaggctgtt gtcgggataa gcaaacgcga gtatctggtc
    15181 gtaacgctct ccctgccccg cctgggccgt ggcttgccgc caggaaagcg tcccttcggt
    15241 agcgcgactg ccatgataat gaaccggatc gcccgcatag ataagatcct gcgtccaggc
    15301 ggtcatcgtt cgcgcccccg tggtcgctgg cgaagcagaa acacgctgcg tggcgctact
    15361 gtcggggatg gtcaggcaat cgccttcccg attggcgttt tgctgtaaaa aagtgcgaat
    15421 cgccacggtc atggctttcg ctgcctcagg gggcgtactt ttcgcctcgc gatccagaac
    15481 tctggcaacg tactcttcac gatcaagatg cgactgcaat ttcagtttgc ccttttcgct
    15541 cagtaaggtc gtctcaccgt ggctgacaaa cgtaatgtgg ttaccattgg caaatgtcac
    15601 acgatagcgc ccattaagta cgccgggttt tactgccgtc gtactctttt ccgctgtgat
    15661 tttcttcagc ggatagcggg caaacagctc cacctctaca cattgcccac tggcaacctg
    15721 cgtcggcact ggcaaaaccc gattaagcac cgtcgcataa cgggttagta ccgttttgct
    15781 ggtgccactg ccagtaaccc acagcggtga accatcggtt aaccagccgg caaaaccacc
    15841 ctgacggatt tcctgtttat catcggcaaa ccagctccag gttttcaccc gtacccgact
    15901 accgagcatg gatgcgacac cgattttcgc ctcgtccagc accacatcca gtaagacttc
    15961 ctgagctttg ttttgcgcgg gtaacgtcgc taatgaatca agcagtgatt taaccgttac
    16021 cgaggtttct ggcttcagcg tagtaagaga cgctaaccac gccggtgcct gacgctgttg
    16081 ccagtattgc ccccacacat ctgcgccaat atgcaaccgc tgcggcgcaa aatagagtcc
    16141 acaggaacgt accagcgcgg tatcgcgcgt tatgctctcc ccagcctgac aacaataaac
    16201 ctcttccggg gagtttccgc gacactggta gggctgctcc ggttgatggg tatcctccag
    16261 ccaggcgtaa acatacagtt tccacaaact ccctaacggc gtggtgagcg tgtcaggtaa
    16321 tgctgagact ttggtcacgc ctgatgacga cagctgatac agttgatcgt gctgcgcacc
    16381 gcgcagcacc agttgtaacg gcgtctcctc cgcaagcgtt ggcagagtaa ccaacgccaa
    16441 tagccagaca attctgcgcc agttcactta tttcacctgc atcccggtcc attcactccc
    16501 tgccgcaaca ctttgctgtg caggtgcata ggaacgcaca taacgagcag gaggcagaac
    16561 gaattgccct ttttgcgaga agcgcagcaa atggcggaaa gtgaccgttc ccgtcagttc
    16621 tttcaccggc accatatagg ccaattcgcc catttcattt cgcgcttttt ccagcaattg
    16681 cccctgctgt ttcgcggcgt tgggtttatt gaccgaaatg ccccatgttg tgcgctcaac
    16741 atcggctccc ggcgggagcg gtacttccac ctgaccatag cgcagaactg catcctgctc
    16801 gctggtaagc gtgatttcat cgagatacag cgcatcgctg tcaatctcat tgctggtcac
    16861 cggctgcaga gtaaagctca tctcttcttc accggggata agccgataca actggcgttc
    16921 aacggtcatc ggaatgttac tttgttgagc cgtttttgcc ggttcacgcc agcggacctg
    16981 cacattttgc ggcgataatt cgtcaccaaa agagagaatg tccggcacgc cctgaccaac
    17041 ccaacgccag tcttcgcccc ctccagttaa cttatgtttg gcccatgcgc ccgcaggcgc
    17101 aggcaacaca accgaaggca ttgtcgccat atatttcgcc agccagttca tggccagcgc
    17161 gcgctcaata gtagattgct cagcggtcaa accacttaaa atagcagccg cctgggtagc
    17221 gtcgcccccg ctattgagca gcacaaccgt gtgcgccagc ggctgattgc tgtttgccgc
    17281 tttatcccac gcatattgct gaacctgtgg catcacagtg gacttcaact gcgctttttt
    17341 cagcaacgtc atggttaaga cgtttgccac cgcatctgcc agtggagatt ccggtgtatc
    17401 gaggatcagg ctgtcattga tatcgcgggt gtcttcctca gagaaatctt cagttttagt
    17461 tccgcgccgg gcgatagctt catccaaccc tttcaacaac gttttgcacg gcagattcat
    17521 ctcctgtgcc cacgccagca ccagcgcccg atgcaataac ggcatgttat ctgcctgctc
    17581 ggcataactg tcgagcatat gctgccagta ttccggttgt tgcgttacgc cgagcgcctg
    17641 gctggcctgc cagtcggcgt accatgccca tgcagtcagg aaggcgtcgc cattgccatc
    17701 ttcaccccac caggtaaagc gtgctccggg ccccgccagt tgcatcagcc gcagacggtt
    17761 atcctgaatc atctgacgaa tgtcgttagc ggcggcactt tgatgatcgg caagcgaacg
    17821 ccaggcgaga ctgagcggga tcagacggct accggtgttg atcaccccac cccacggttc
    17881 atcgactaac gcatcaagat tgttgcggaa aatctcctgc ggcgtttcac tactttgtag
    17941 ccggatattg ctcgcctgct cgggcaatat cagcgcgttc tcgccaccgc cgagcatgac
    18001 atttttctgc tgttcaacgg gccagctgtt atccacaaaa gacagttttg tgctaatgct
    18061 gtcctgcact tgcccatttt gttgcagttc tgcacttaac aagccagatt gctgaatatt
    18121 ctgcgtcagc gaaatataat tcgccccttt gtgcagcgtc agcgtctggc gcatctcagc
    18181 gcctgcaaat ttagtcacca gcgccaccgg ttcgttatcc tgctgactga agataaacag
    18241 tcctgccgcc ggtttgtcgc ccacgcgata caccgttggc atactccact tcatgtagag
    18301 atttttttcc gaacgaagat aagcacgccc ctgcccgacc agcccgtcgc cgttcatccc
    18361 tctggctgta atgcgccaac gggttaacga atcaggcatc aggaacgtga aatacgcttt
    18421 gccttgttta tcagttgtga gtgacggcat ccatgccgcg gtatccacct cttcacgccg
    18481 tggacgttca agcattttta ctcgccgctc gctgcggtta gtcgcgcctg gcgcaaccgg
    18541 ctcgctggag agcgcctggt cgtagctgat aaacgaaaga ctggagctgg tacgcacatt
    18601 gttacgcccc agcggataga aaaatttgcc gatattcggc gcgatttccg gttgcagcgc
    18661 atagatcatt tcatcgacca cgccaaccgt tagctgcgca gaaacaggtt tacctttcag
    18721 caacgaggtt aattcgacat tgaccagttc accaggctgg taatgggttt tgtccgtttt
    18781 cacccggata tccagctggg gaacggcaac tttgatcccg gcgttctgaa aactgtactg
    18841 accgttacgg gtatacagca ccgaaaaagt gatattaggc gcaaaggaat tgctcaccgg
    18901 tacgcgggct tcatactggg tatcgtttaa acgttgtaac gtcagccagt ttgccggatg
    18961 cgatagcagc gactgctgtt caacgcgatc gcgttccagc gtcaataatg cttcatcaat
    19021 tggctccggg aaggtaatca gcatcttcgc ggtttcgcca ggctggtaca gcgttttatc
    19081 tgccacaata tctaccgtac cagtatgcga cgtgctgccc ttaccgctga ctgcatggct
    19141 taacccggcg agaattaagc cgtctttatc gcgtaacgtc agattgtagt tacccggttt
    19201 atcgaaattg acggtaaagg atttgccacc agaaggtaga tctccgctgt ggcttgttcg
    19261 gtcttccagg cgtagccact catacttaac aggcacctgc tttgaagatt ccagcgcggc
    19321 ataacggaaa acaaccgact cgccgctatt actgtattgt gcggaggtgc ttagagagta
    19381 atgcgccaga ccgcgttcaa tgaggatctc tttggtagta gtaacacgat atgccgcgcc
    19441 atcgctggcg gagacggtca gtaaatagcg gctcggttta tcggcggcgg gaaggtttaa
    19501 cgctacattg cctttatcgt cagaaacagc gtcgctacct tccagcgaca ccgggaaacg
    19561 tccggcataa cgcaaatcgt taccaaccat cgaaagttgc tgagcgcgca aactcaactg
    19621 cacgctggca tcttttaccg gttcgccatc cggatagagt agttgcagtt tgccgctgac
    19681 cgcttcgcca gttttgaaat cttttttgtc gagggctaag ccaatctcaa aatgtggttt
    19741 gatgtagttg gctacgcgaa aactgctgct atagacctgt ttgcggtaag ccaggcgtaa
    19801 ctcataacct ccggcgacgg cattttctgg caggcggaaa ctcccctgcc cgccattacg
    19861 cgcatccagc gtgacattga tggtttgcaa cagactgccg ttggcgtcca gcactgaaag
    19921 cttcgccggg gcgctaacga tgggggatga atgcaacggg tcgtggaact cgcggcccat
    19981 cactttgaca tcaacacgat cgcctgcgcg atacagaggg cggtcggtga agatatacaa
    20041 gcgggtgttg taaatttcgc tttcgtagaa aaaattctcg gagacaaaaa cgccgccttc
    20101 agcatcttta cccagaatgt atgaacgttc cggcgatata tgttgtaact gcaaggtacc
    20161 actgtcatcg gtcacaccgc gggtcatcac gccaagaccg tcagtccaca agatctctga
    20221 tcccggcttc gcttcaccct gttttttacc cgcggtccac accaggagct cgttacccga
    20281 cactttgcta agcgccacgg tatcagagac aaataccacc gtcgtcgccc gatatccacc
    20341 gaccatcgcc tcgacgaggt acagtcccgg ctcttgtttg ccgagaggaa tatagatgtt
    20401 acccggctgc ggcgagatga aattgctgga tgcgccttcc agttttaccc cttgctgcgg
    20461 ctcgaccggt ttagcctgcc atagtgggta acggaactgt tccaccagtg gatatttttt
    20521 cagcggtgaa aactggctgt tctgtacata acgggaaggt ttaataatgg cattgccgag
    20581 ctgtaattcg ggtaacgcct gagtcacatt ctgccgtgac tgagaagaga aagtacgctg
    20641 catcacgcgg cgagatttgc cgtaccagtt atcccacagc caggtcagcg tattgttcag
    20701 cccgtcgccc agatattgcg gttgcaccac aatgcgatgc aggtttttct gctggcgcaa
    20761 aaatgccatc gggtcaggaa tacgatacag gcgaacgtcc acgccgccgt actcttccat
    20821 ctgatagcgc cgataatcac gccccggcgc ttccagtcgc actttcgcct cttcactgct
    20881 gctaaaactg ctgtcagcaa gcaaaaagaa tgttccccct gcgggcggcg catagttact
    20941 ggaaggaagc gaatcatcag cattagcaag ccctgttccc acaagagaca gggacaagca
    21001 cgctgctatc gcgccagaaa atttaaacga taaatgccaa tgaaattggg attggaatcg
    21061 ttgggtatcc atcgggtgtc cttccatgtc ataagttgtt gcagactgac tgcgcgcatt
    21121 ccgttgtcag ttttcgtggc gcttccggtg tggtagatga cgtaacgccc catccagacc
    21181 attaagtgct gggcatcgcc ctgatcgaaa aaaatcatat cgccaggcag cgcctggttt
    21241 atgtcctggc caatgaactg gctgttgtac tgaatcaaat taatcgcggt cacatagggg
    21301 ccggtttttc cgttcccctg attccagttt tgcgccagtt gacgctgttc aggtgttagc
    21361 gtcatttcag gcggcaaata ctgacttgct atgccgttac tttttagcca tttgctgtcg
    21421 tgaactttca gcgcctcgtt cgccgaaaat cgcaccaggc ccgcacaatc ctgctgatac
    21481 cagcgcggac ttggcccctg tcggagctgt tcctgtgcaa tacgcacaaa ccaggcgcga
    21541 aacagccccg actgttcgac attcagcatt tcgctatggg caacaacaca acacagccag
    21601 caaatcagcg ccagcagtcc gtgcctcata gcggctgcca ggttatgggt agccactgcc
    21661 aggcggcatc gggttccatc tgtgccagct tcatgacata acgcggttgt tgagataaag
    21721 cgtccagctt cggcattaat aaagtttgtg cggcgttata aaaaaccggt tcgagattct
    21781 tcggcagact ggtcagcgtt tcgttacgca gcagtttcgc cacgccttgt ggattgatat
    21841 agagcggaac gatgccgtca gtgggtatta catccaccat tgccgggcgg gttttattca
    21901 gtgtttgcag cgcgttatta accagcgtgt catcgaggga gaaaagcagc gttttgtttt
    21961 gcatcgccag cgacacgcgg aaaaaataat ccgacattag ttgatctggt tgcgccgcct
    22021 gtgttttcgg atactggccg tatcgggaac tcacttcgcg acgccagatt tgcgcttcgc
    22081 cctgctgagt ctggtttacc ggcaaaacac cttctggcgc tttgctttcg tgcgcaccaa
    22141 tattttgcgt aaacagtttc ccaggcagtt gtgcctgttc ggcagtgcca tcaaactgac
    22201 cgacaaacag cggggtttgt aattttgagt cttcatacca gcacagcccg gctgcaccat
    22261 ctaacgcccc gttcagcttg tcgttttcct ggctgatgtg cgaaagcatc tcttcggcaa
    22321 taccatgcga atacggcacc gccacacaga agctggcccc ggcaggcata ctgttccata
    22381 ccggcgtaaa atcgaaactg gcatctacgc ttgcggattc atcatttaac gccacaaagc
    22441 tatgccagcc gtcgttgctc atttcgaagc gtacgccagc aaaagaaggc attaatcgct
    22501 ggtagccaaa ccccagcaac ctggcgctga ccacgatgcg ctggcgtact ggcgtttttt
    22561 cggcagtacg ctcttccagg ccaaagctgg cttgccagcg ttttttaccg ctcaacaaat
    22621 cacttgcgat cgccgtggct tcggtatcct gctggtcatc tttaaacagc atatccgtac
    22681 tggaaaacac cagcatcttg tcctgataag tcgcgaacat cagggcgtta ttgccgttat
    22741 agcgcaactg ataaaccggg atggtttcac tatttagctt aatactactg atttccgttt
    22801 tgcttaactg gctgtcgctg gtagcggcaa acaacaatgg ctccagcagt ttgcttaaac
    22861 cactgcgctg gatcagcacc atgtaatgtg aaagatggcc ttgtttatcg tgccatagcg
    22921 ccgcctgcgc gggctgatct aagagtgacg aaaagagctt atctttcagc gtgagatcgt
    22981 gttcatagac aatgcggcga atgctgcctt caatgcccag acgatcggca tgattctgat
    23041 aatagaaaac gaaatcttca ctcagaacat cgtggagaaa cggaatggta aggaggtctt
    23101 tgggaagctg gctcagagag tcgctgtcga gaaagaggtc cggctcattg agatcgattt
    23161 gcagattgtt gtgcaccacc agcggcgaca acgttttttc tggcccactg ccagcatatt
    23221 gcaacgccca gacgccagcg gaaagcagta ctattgcgcc caaacctaca agaccataga
    23281 accgccagcc tttcgccttt ttttcaccac tcattgccac attccttgtg taaagccagc
    23341 cattttttat gagcacagca aaactttacc gcgccctaat acgacaaaag cccagacttt
    23401 gcagcctgga cttttcaatt caaacaaggg agatagctcc cttttggcat gaagaagtaa
    23461 aattattctt cttctggctc gtcgtcaacg tccacttccg gagcgatttc atcgtcccct
    23521 tccgcggcac tgccgtcgat ggtatccaga tcttcctcgt caaccggttc agcaacacgt
    23581 tgcagaccca ctacgttttc atcttccgca gtacggatga ggatcacgcc ctgggtgtta
    23641 cggcccacga tgctgatttc cgaaacgcga gtacgtacca gcgtaccggc atcggtgatc
    23701 atcatgatct ggtcgcagtc atctacctgc accgcgccaa ccactaaacc gttacgttcg
    23761 gtaaccttga tggagataac ccctttcgtc gcacgcgact tagtcgggta ttccgccact
    23821 gcggtacgtt taccataacc gttctgcgtt gcggtgagga ttgcgccatc gccacgaggc
    23881 acgatcagag agacgacttt atcgccttcg cctaaacgaa taccgcgaac accggtggtg
    23941 ttgcagccca tcgcacggac agaagactct ttaaagcgca ccactttacc ttcggcggag
    24001 aacagcatta cttcgtcttc gccgctggtc aggtcaacgc cgatcagctc atcgccttca
    24061 acaagtttga tcgccacttt accggcggta cgcagacggt tgaactcggt gaggacagtt
    24121 ttcttcacgg taccgttagc ggtcgccatg aagactttca cgccctcttc aaactcggtc
    24181 accggcagga tggcagtgat acgttcgtcc tgctccagcg gcagcaggtt gacgatcgga
    24241 cgaccgcgcg cgccacgagt ggcttccggc aactgataga ctttcatcga atagacgcga
    24301 ccacggctgg agaagcacag aatatggtcg tgggtgttcg ccaccagcag tcggtcgata
    24361 aagtcttctt ctttaatacg tgcggcagat ttacctttcc cgccacgacg ctgcgcttcg
    24421 tattcagaaa gcggctgata cttcacgtag ccctggtgag agagtgttac caccacatct
    24481 tcctgtgtga ttagatcttc caggttgatg tcggcgctgt tggcagtgat ttcagtacga
    24541 cgtttgtcgc cgaactgttc acgaaccagc tccagctctt cacggatcac ttccatcaga
    24601 cgatcggcgc tacccagaat acgcagcagt tccgcaatct gatccagcag ctctttgtat
    24661 tcgtcgagca gtttttcgtg ctcaagaccg gtcagtttct gcagacgcag atccagaatt
    24721 gcctgggctt gctgttcggt cagatagtac agaccatcgc gcacgccgaa ctctggctcc
    24781 agccattccg gacgcgcagc atcgtcgcca gcacgttcga gcatggcggc aacgttgccc
    24841 agctgccacg gattagcaac cagcgcagtt ttcgcttctg caggcgtcgg cgcatgacgg
    24901 atcagttcga tgatcgggtc gatgttcgcc agcgccacgg ctaatgcttc aaggatatga
    24961 gcacgatcgc gagctttacg cagttcgaaa atagtacgac gggtcaccac ttcacggcgg
    25021 tgacgaacaa acgccgcgat gatgtctttc aggttcatga tcttcggctg accatggtgc
    25081 aatgccacca tgttgatacc gaaagaaacc tgcaactggg tctgggagta gaggttgttg
    25141 agcacaactt caccgaccgc atcgcgtttc acttcaatca cgatgcgcat accgtctttg
    25201 tcagactcgt cacgcagcgc gctgatgcct tccacgcgtt tttcttttac cagttccgca
    25261 atcttctcga tcaggcgcgc tttgtttacc tgatacggaa tttcgtggac gataatggtt
    25321 tcacgtccgg ttttggcgtc aacttccact tcagcacggg cgcggatata taccttgccg
    25381 cgaccggtac ggtaagcttc ttcaataccg cgacgaccgt tgatgattgc cgccgtcggg
    25441 aaatctggcc ccgggatgtg ttccatcagc ccttcaatgc tgatgtcttc atcatcgata
    25501 tacgccagac aaccgttgat gacttccgtc aggttgtgcg gcgggatgtt ggttgccata
    25561 cctacggcga taccggaaga accgttcacc agcaggttag gaattttggt tggcatgacg
    25621 tcgggaattt tttccgtgcc gtcatagtta tcaacgaaat cgaccgtctc tttttcgaga
    25681 tcggccatca gttcatgggc aattttcgcc agacggattt ccgtataacg cattgccgcc
    25741 gcagagtcgc cgtcgatgga accgaagtta ccctgaccgt ctaccagcat gtaacgcagc
    25801 gagaatggct gcgccatacg gacgatcgtg tcataaaccg ccgagtcacc atggggatgg
    25861 tatttaccga ttacgtcacc aacgacacgg gcagattttt tataggcttt gttccagtca
    25921 ttgcctagta cgttcatggc gtaaagtacg cgacggtgta ccggcttcag gccatctcgg
    25981 acatccggca gcgcacggcc aacaatgacc gacatcgcat aatccagata ggagctcttc
    26041 agctcttcct caatgttgac cggtgtaatt tctctcgcaa ggtcgctcat ctaaccgcta
    26101 tccctctact gtatcccgga ttcaaaggtc gcaaattata acacagccgc gcagtttgag
    26161 gtaaacctat acgctttatt cacatccaat gcctgatata ctcgtttgtc ttgccaatta
    26221 cggagtagaa gtgccaatga atgccgaaaa atcgccggta aaccataacg tagaccacga
    26281 agagatcgct aaatttgaag ctgtcgcctc ccgctggtgg gatcttgaag gtgagttcaa
    26341 accgctgcac cgcattaacc cgctgcgtct gggctatatt gccgagcgtg ctggcggttt
    26401 atttggcaaa aaggtgctcg atgtcggttg tggcggcggc attctggccg agagtatggc
    26461 gcgcgaaggc gcgacggtga ccggtctgga tatgggcttt gagccattgc aggtggcaaa
    26521 gttgcacgcg ctggaaagtg gcattcaggt agagtacgtg caggaaaccg tggaagagca
    26581 cgcggcaaaa catgccgggc agtatgacgt ggtgacctgc atggagatgc tggagcacgt
    26641 ccccgatccg cagtcagtgg tcagagcctg tgcgcaactg gtgaaaccgg gcggcgatgt
    26701 ctttttctcg acgcttaacc gcaacggcaa gtcatggctg atggcggtgg ttggcgcgga
    26761 atatattttg cgcatggtgc ccaaaggcac gcacgacgtg aagaaattta taaaaccggc
    26821 agaactgctg ggctgggtgg atcagaccag tttaaaagag cgacatataa ccgggctgca
    26881 ttacaacccg atcactaatt cctttaagct cggccccggc gtcgatgtga actatatgct
    26941 gcacacgcag aataaataaa attgttagcc ggataaggtg ctcgcaccac atccggctac
    27001 aatgccgggc gcaaagcagc taccatttca ccgtcatcga caaaaaccct gccgtctggg
    27061 caaaatcatc actccctttc tgccacgcca cgctgccgcg cagcgaaact cgctgactga
    27121 tattgcccgt gactcccact tttatttcac cccgttgctt caccgcatcg tcactgatag
    27181 tgctgccatc ttcttcaatt tccgtcgcat ggggatcgtg ataataattc agatctaatg
    27241 atggtatgac atgaacagcg gtacgccatt cgctgtgtaa acccagccgc gtttgaatat
    27301 catcaccctg cgattgtgac acgcgcgcat ggttagcggc ggtaaaatca tcctgctgca
    27361 cgccctgata aatcacctgc gcctgtggtt caatcaccac tccatgcccc ggtaaccact
    27421 gatacccggc ttccagcgag gcgataatcc ccgatgagtg gtaatgatcc acgccatctt
    27481 catgttcgga aacatcattg ttaaaccacg cgtattgcag ccagctatcc agccaggccc
    27541 cctgcttctg attaccgtgc tgaaaccagc ttgaggtcag accaacggca taaccgtggt
    27601 tctggttatc ggcgcgagtt ccggtcatat tcgagcggct gtcgccctgg ttatcactgt
    27661 agccaccaac cgccccaagc atccactcgc catccgcgcc ccagcgtccg ctaaacaagt
    27721 cgccgctaag ctgcaccgta gaagtatctt catgctgagc cagttgcccc gctgctgtgt
    27781 aatgataatc tccgccgata atacgtaaat tcagcgtctg accatcgcca ccagcgtgat
    27841 cgtgtcgctc catcacaaac gcctgatttg ccgcccgcag gttattaaaa tatccaccaa
    27901 ctttggcatt caacaccggc tggtaggcag gcgtaggttc cgggtcgggt gttggatcag
    27961 gagtcgggtc tggatcaggt ggcgatggtg gcgttgcttc ttgcgatcgc agataccagt
    28021 cgttgttatc ttccaccagc gtgtagtcat acgctcccat attgacgtag ccgctgcccg
    28081 ccagactgaa ctgcgcattg ttttgaaatt gcgttggatc ggctgcgaaa tcaaccactt
    28141 taatgcctgt agatgtcggc tcaccaatac ccgtaatggg attaattacc acggtcgtgt
    28201 tgccagcagt attaccgttc aacaccagtt ggtcacttgc cgagtcatcg ccgtttaatt
    28261 cgctatcgag cagtagcgta ccaccgccgg tataatcacc gtttaccgtc agcgtgtcgc
    28321 cggcgacgcc attttgcagg ctgaccgcac cggacgtatt ggtcagattg ccattaactg
    28381 ttacgtcgga aagataagtc aggctgtcag tttcaaggtt ggtgttaagc tcgcctgtca
    28441 acgtcacgcc atcggtggca ttcaccagcg tcccatcgcc gttaaacagt gaggcattaa
    28501 gggcgacgga agtatcctgc ccggtcaggc gcaaaaccgt accatcgctg atgtcgatag
    28561 tgccgctgga agtaacatca attgactgaa tatcctgatc cgcgccagtc ttgaacgttg
    28621 ccccgtcacc gacccacagc gacgaagcat aagggaagat atccgcaaca tcgcctttca
    28681 gggtgccttc ttcgacacgt accgccgact gcgtcgtacc gctggcggtc agttccagcg
    28741 tacccgcccc cgttttagtc aatgtgctac cctcatcctg atgctgcccg ctgctgtcag
    28801 ccatcagtcc gccccactgc gtgtctaccc ccgcatcaac cgccacttca ccgtcggcgc
    28861 gcatttcaat atcacgcccg tgacctgctg cctctgaacg agtatcaacg tcagcggtaa
    28921 tttccatctc actttgttgt tggctatcag taaagatgag ctgacggttt tcgtcggtat
    28981 tgccaagctg cgagttgtcg ctcaccacca gcgtaccgga agcgatttgc gtggtgccca
    29041 ggtaactatt attgtttgcc aggctaacca gcccgtcgcc cgcatcgatc accacgtaga
    29101 cgccatcttt cccgtcgccg ccgatatgca aattgtcgcc gcctgcgaga tccaccacat
    29161 cctgactgcc gataaccgta ccgccgctgc cgctgacttc aaggccatcg ttgtatgatg
    29221 tgccgctctg ccaggtggcg aaatcagaga gatcaagcac gccgccgttt aacacgatcg
    29281 actgcggatc gtcctggaga gcggcaagat ctgccgcgtc gccttccagc gtaagcaccg
    29341 cgccatcatc aacgactata tcgccagtta gcgccatcga ctgtgcccct gccagcacgt
    29401 agctgccgtt ttgcgcaatg gtgagctgac cagcaccttc gatggtgcca gcaaaactgc
    29461 cttgattaac agtaacattg ccaccagcat cgatatttaa agtgccattc tgaaagcccg
    29521 tcaatgcgtg cacaaaagtt tgttgggtcg agccaacgtt tagctcagcc tgattctgat
    29581 actgatcaat actccctatc gtcagaccgt agcagtcttg cggatcgtcc tggcaatgcg
    29641 tatcgccgac attcatcagg gagttgctgc ggcccagggt aacttcaccg ttttctatct
    29701 gcatctcgcc agtaaagtca ttgttatttg cattaagtac cagatcgccg gaacctgttt
    29761 tggtgattac cccggtacca gcaatagagt caacagctcc gtcattctct gtattgccga
    29821 taaccagcgt ttttccgtcg gcaatatcaa aggtaacttc acttaagccg agatacataa
    29881 agccgcccgc cgcagtggaa ggaccatccc cataacctgc tgcgctgttg ttctcatcca
    29941 ctaacacgcc gttgttctgg ctgtagctgt catcaactga aatatcaata agatagggag
    30001 ccgtcacgct attggtataa atcgccccac catatccctc agcagagtta tttgtaaatg
    30061 cggtgttatt tattattgtg aaacctgacg gatgcgtgat attgctatta ttatcggtga
    30121 catcgattgc ccctccatcg ccatcactgt aacttgttga tgtatatgcc tggttgttat
    30181 caaaaataac ttcacttaaa tagacgtcat tgttgatggt ataaattgca ccacctttgc
    30241 cgtcattagc gatgttattg cgaaacacgg cgttagtgac acttaaatcg acgacaccgg
    30301 tatcgttagt accagaagaa tagattgcgc caccatagcc acctgcaacg ttatcggaaa
    30361 aaataacatc agtaagattc agcgttgagt tttctttagc aaatattgcc ccgccgttat
    30421 tatattctcc tgagacagta ttattggcaa acagagtcat tccagtttca ttttctggcg
    30481 atagagaaaa ctccgctcct tgttgcaaaa atacggcccc accgctggcg ttattgttca
    30541 tatccgaaaa aaccagccat tgcccatcgg cgatgctcca gtcctgcgta atgcctgaaa
    30601 ggctttgcct gctggcgtga caactcgctt tgatatcata tccctgacat gaatcaatga
    30661 ccgccgcgac accgttagca gagaaaagag atgcaatcat tgacgggagt aaagataaat
    30721 actccttgcg tagaaaaata atccgcatat taatctgacc atcattttct ataagacggc
    30781 atatttaatc gcattatacg atatgggtta tttttctttt gtaaattgtt caacccggtg
    30841 caatggctat tttgttatta tttcatgaat aatatcagt
//
LOCUS       DACBVI010000043        30164 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.043, whole genome shotgun sequence.
ACCESSION   DACBVI010000043 DACBVI010000000
VERSION     DACBVI010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.043"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..1512
                     /gene="mltF"
                     /locus_tag="IU325_09385"
     CDS             <1..1512
                     /gene="mltF"
                     /locus_tag="IU325_09385"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HAP5809239.1"
                     /translation="LLLAVALWPSIPWFGKADNRIAAIQARGELRVSTIHTPLTYNEI
                     NGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNADLLAAGLVYNSERVK
                     NYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVVVNDLQTLKETKFPEL
                     SWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELAVALDITDEQPVTWFS
                     PLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVDTRTFLRAVDAVLPQL
                     KPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLTKNTAQSLGITDRTDA
                     EQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMLDARALTAKTKGNPDS
                     WADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQISLVGYLQEKEKQATEA
                     AMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLFSRKGSEEKQN"
     gene            complement(1509..2045)
                     /gene="tadA"
                     /locus_tag="IU325_09390"
     CDS             complement(1509..2045)
                     /gene="tadA"
                     /locus_tag="IU325_09390"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP5809240.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(2070..2705)
                     /gene="yfhb"
                     /locus_tag="IU325_09395"
     CDS             complement(2070..2705)
                     /gene="yfhb"
                     /locus_tag="IU325_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HAP5809241.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            2914..3762
                     /locus_tag="IU325_09400"
     CDS             2914..3762
                     /locus_tag="IU325_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP5809242.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3818..4078
                     /gene="yfhL"
                     /locus_tag="IU325_09405"
     CDS             3818..4078
                     /gene="yfhL"
                     /locus_tag="IU325_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HAP5809243.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
     gene            complement(4273..4353)
                     /gene="shoB"
                     /locus_tag="IU325_09410"
     CDS             complement(4273..4353)
                     /gene="shoB"
                     /locus_tag="IU325_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HAP5809244.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(4773..5153)
                     /locus_tag="IU325_09415"
     CDS             complement(4773..5153)
                     /locus_tag="IU325_09415"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP5809245.1"
                     /translation="MAILGLGTDIVEIARIEAVITRSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(5153..5884)
                     /gene="pdxJ"
                     /locus_tag="IU325_09420"
     CDS             complement(5153..5884)
                     /gene="pdxJ"
                     /locus_tag="IU325_09420"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HAP5809246.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLAEAGIQVSLFIDADEGQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(5896..6624)
                     /gene="recO"
                     /locus_tag="IU325_09425"
     CDS             complement(5896..6624)
                     /gene="recO"
                     /locus_tag="IU325_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP5809247.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(6636..7541)
                     /gene="era"
                     /locus_tag="IU325_09430"
     CDS             complement(6636..7541)
                     /gene="era"
                     /locus_tag="IU325_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP5809248.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(7538..8218)
                     /locus_tag="IU325_09435"
     CDS             complement(7538..8218)
                     /locus_tag="IU325_09435"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003848606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP5809249.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(8491..9465)
                     /gene="lepB"
                     /locus_tag="IU325_09440"
     CDS             complement(8491..9465)
                     /gene="lepB"
                     /locus_tag="IU325_09440"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP5809250.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(9481..11280)
                     /gene="lepA"
                     /locus_tag="IU325_09445"
     CDS             complement(9481..11280)
                     /gene="lepA"
                     /locus_tag="IU325_09445"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP5809251.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(11478..11957)
                     /gene="rseC"
                     /locus_tag="IU325_09450"
     CDS             complement(11478..11957)
                     /gene="rseC"
                     /locus_tag="IU325_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HAP5809252.1"
                     /translation="MIKEWATVISWENGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLMGLFLIASLFQLLFAS
                     DIAALCGAILGGVGGFLIARGCSRKFAARSEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(11954..12910)
                     /gene="rseB"
                     /locus_tag="IU325_09455"
     CDS             complement(11954..12910)
                     /gene="rseB"
                     /locus_tag="IU325_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HAP5809253.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(12910..13560)
                     /gene="rseA"
                     /locus_tag="IU325_09460"
     CDS             complement(12910..13560)
                     /gene="rseA"
                     /locus_tag="IU325_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HAP5809254.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(13593..14168)
                     /gene="rpoE"
                     /locus_tag="IU325_09465"
     CDS             complement(13593..14168)
                     /gene="rpoE"
                     /locus_tag="IU325_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HAP5809255.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(14165..14320)
                     /gene="rseD"
                     /locus_tag="IU325_09470"
     CDS             complement(14165..14320)
                     /gene="rseD"
                     /locus_tag="IU325_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HAP5809256.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            14576..16198
                     /gene="nadB"
                     /locus_tag="IU325_09475"
     CDS             14576..16198
                     /gene="nadB"
                     /locus_tag="IU325_09475"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HAP5809257.1"
                     /translation="MNTLPDHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGGGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGAESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNICVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMYLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(16183..16920)
                     /gene="trmN"
                     /locus_tag="IU325_09480"
     CDS             complement(16183..16920)
                     /gene="trmN"
                     /locus_tag="IU325_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HAP5809258.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAKRINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECSTPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            17052..18386
                     /gene="srmB"
                     /locus_tag="IU325_09485"
     CDS             17052..18386
                     /gene="srmB"
                     /locus_tag="IU325_09485"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HAP5809259.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(18419..19300)
                     /locus_tag="IU325_09490"
     CDS             complement(18419..19300)
                     /locus_tag="IU325_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP5809260.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIKDVDFTEPGRHNACSFIINEPQCVFRQLFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFTVANELASGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHIFIQCVEECFLPG"
     gene            19403..19990
                     /gene="eamB"
                     /locus_tag="IU325_09495"
     CDS             19403..19990
                     /gene="eamB"
                     /locus_tag="IU325_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HAP5809261.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFRQSTRVLA
                     GMSLGFLIVMLLCVGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRPLNIVLALLLVYCAVRIFY"
     gene            complement(20052..20435)
                     /gene="grcA"
                     /locus_tag="IU325_09500"
     CDS             complement(20052..20435)
                     /gene="grcA"
                     /locus_tag="IU325_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HAP5809262.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            20740..21429
                     /locus_tag="IU325_09505"
     CDS             20740..21429
                     /locus_tag="IU325_09505"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP5809263.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGEKPI
                     DWMPVLPAESE"
     gene            complement(21477..22514)
                     /locus_tag="IU325_09510"
     CDS             complement(21477..22514)
                     /locus_tag="IU325_09510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAP5809264.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSHPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDEALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            22721..23140
                     /gene="trxC"
                     /locus_tag="IU325_09515"
     CDS             22721..23140
                     /gene="trxC"
                     /locus_tag="IU325_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HAP5809265.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            23209..23907
                     /locus_tag="IU325_09520"
     CDS             23209..23907
                     /locus_tag="IU325_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HAP5809266.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSLDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            23939..26599
                     /gene="pat"
                     /locus_tag="IU325_09525"
     CDS             23939..26599
                     /gene="pat"
                     /locus_tag="IU325_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HAP5809267.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            26713..28068
                     /gene="pssA"
                     /locus_tag="IU325_09530"
     CDS             26713..28068
                     /gene="pssA"
                     /locus_tag="IU325_09530"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HAP5809268.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            28114..28437
                     /locus_tag="IU325_09535"
     CDS             28114..28437
                     /locus_tag="IU325_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HAP5809269.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YAQHQGMSRDRSATFGLMFSVGLGASKELWDSRPEGSGWSWKDFTWDVAGATTGYTVW
                     QLTRH"
     gene            complement(28434..29732)
                     /gene="kgtP"
                     /locus_tag="IU325_09540"
     CDS             complement(28434..29732)
                     /gene="kgtP"
                     /locus_tag="IU325_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HAP5809270.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(30041..>30164)
                     /locus_tag="IU325_09545"
     CDS             complement(30041..>30164)
                     /locus_tag="IU325_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP5809271.1"
                     /translation="VEELENAVNEYITYWNQKRIKLSLGGLSPVEYRTEYQKAG"
BASE COUNT         7389 a         7913 c         7491 g         7371 t
ORIGIN      
        1 ctgctgctcg cggtcgctct ctggccatcc attccctggt ttggtaaagc cgacaaccgt
       61 atcgccgcca ttcaagcgcg gggagagttg cgtgtgagca ccattcatac tcccctgacg
      121 tataacgaaa tcaacgggaa accttttggc ctggattacg aactggcgaa acagtttgcc
      181 gattacctcg gcgtaaaact gaaagtgacc gtgcggcaga atatcagcca gctgtttgac
      241 gacctcgata atggtaacgc cgacctgctg gcggcaggcc tggtctataa cagtgagcgt
      301 gtaaaaaatt atcagcctgg ccctacctat tattccgtgt cacaacaact ggtttataaa
      361 gtgggtcagt atcgcccacg tacactgggc aacctgacgg cggagcaact caccgttgca
      421 ccgggtcatg tggtggttaa cgatctccag accctgaaag aaacaaaatt cccggaatta
      481 agttggaagg tagacgacaa aaaaggctct gcggaattaa tggaagatgt catcgaagga
      541 aaactcgatt acaccattgc tgattctgtt gccattagcc tgtttcagcg cgttcatccg
      601 gaactcgccg tagcgctcga cattaccgat gaacaaccgg tgacctggtt tagcccatta
      661 gatggcgata ataccctttc cgccgctctg ctcgatttct tcaacgaaat gaatgaagac
      721 ggtacgctgg cacgcattga agagaaatac ctggggcatg gcgatgattt tgattacgtc
      781 gatacgcgca catttttacg cgccgtcgat gcggtactgc cgcagttaaa gcccctgttt
      841 gagaaatacg ccgaagaaat tgactggcgt ttgctggccg ctattgctta tcaggaatcg
      901 cactgggatg cgcaggccac ttcaccgacg ggtgtgcgcg gcatgatgat gctaaccaaa
      961 aataccgcgc aaagcctcgg cattacggat cgtaccgatg ccgaacagag catcagcggc
     1021 ggcgtgcgtt atttgcagga tatgatgagt aaagtgccgg aaagtgtgcc ggagaacgaa
     1081 cggatttggt ttgccctcgc cgcgtacaat atgggctatg cgcatatgct ggatgcccgc
     1141 gctctgacgg caaaaaccaa agggaatcct gacagttggg ctgacgtaaa acagcgtctg
     1201 cctttactta gccagaaacc ctattacagc aagctgactt acggctacgc tcgtgggcat
     1261 gaagcctacg cttatgtcga aaatattcgt aagtatcaga ttagcctggt gggttatctg
     1321 caggagaaag agaagcaggc tacagaagcg gcgatgcaac tggcgcagga ttatccggcg
     1381 gtatcgccta cggagctggg caaagagaaa tttccttttc tctcgtttct ttcccagtcg
     1441 tcatcaaact atttgaccca ctctccctct ctgctgtttt ccaggaaagg gagtgaagag
     1501 aaacaaaatt aatccgtcga ggattgcgct tttttctgcg ctttaatttc ctggcggcgc
     1561 atgcgaaaga agtcactgag caacgccgcg cactcatccg ccagtattcc ttcggtaatt
     1621 tccactcggt gattcatacc cggatgatgc agcacatcca ttaaagatcc cgcagcgcca
     1681 gttttcgcgt cacgcgcacc aaagaccacg cgaccaatgc gactgtggat catcgctccg
     1741 gcacacatta cacagggttc aagcgtgaca tacaacgtgg cgtcgatcag acgataattt
     1801 tgcatcacca gaccgccctg ccgcagagcc atgatttctg catgtgcggt gggatcatgt
     1861 cgaccaatcg gacggttcca gccttcgcca ataacacgat tgttatgcac taataccgcg
     1921 ccgaccggca cttcccgctc atcccaggca cgctttgcca gcgtcagcgc gtgacgcatc
     1981 cagtattcgt ggctaaattc gacttcagac aaaaagaaaa ctccggttat aaaagcgcgg
     2041 cgcattatac acggacgcta tgctttactc tattccagtt gctggagttc accgcgcggg
     2101 gttactcgcc aacgatgctg acagaaataa agcagcgggt tgtcctgatt actgtcgcta
     2161 tagccactgt acaaccgcag tggcgtgccg attttacgct ccagttgcgc gaccttctca
     2221 tgcccgaggc aacgcatcgt caatacccaa ccaccatagc cacgctgaat ctgactggcg
     2281 ataagattaa cccgcggcag ccagggcgta tcgaaataaa ccgcttcaac cagcggctgc
     2341 ggagagccgg taatcaacca gatatcagca tcggaactta acaggtaggt ggttaatcgc
     2401 tcctgaacca gcggaaaggc ggtaacattg tcacgaaacc agcgtacgaa atcggcctgc
     2461 aacgcctgca aacgcgcttc attgtgacca aaagtgcatc cccacagaag cagactcatc
     2521 ggccagcgtg ccgcacggcc ttttatcaat aacgcaatgg ctatgatcgg caacaacggc
     2581 aggacaagca acgcattcag cggttggcgc cgcagtaaat aacgcaaaaa actgccgaac
     2641 atatcctgct gatgcaatgt tccatctaag tcaaaaaaca ccacacgacg ctcgtgagtt
     2701 gccaaaccat actcctctgg actgaaacat cctgattaat tttcgctact acatagccta
     2761 acagatagat catcactttt ccggcaacaa tcgaccgcat catggctaac tggaattttt
     2821 aattcatgga aattagcggc aatggaatat aaaattcact cgcgtttgtc tcatattgcc
     2881 tgttgtcgcc actatttcat cgcgaggaaa gatatgaacg gcttacttcg tatccgtcag
     2941 cgttaccagg ggcttgccca gagtgataaa aaactggcgg attatctgct gctacaacct
     3001 gatacggcgc gccatttaag ctctcagcaa ctggccaacg aagccggagt cagtcagtcc
     3061 agcgtcgtga agttcgcgca aaaactcggc tataaaggtt ttccggcgct taagttggcg
     3121 ttgagtgaag cgctggcaag ccagccggaa tcgccctccg tgcccattca taaccaaatc
     3181 cgcggtgatg atccgttacg gctggtcggc gaaaaactga ttaaagaaaa tactgccgct
     3241 atgtacgcaa cgctaaacgt taatagtgaa gagaaactgc atgaatgcgt aacaatgttg
     3301 cgctctgcgc ggcggataat tctgaccggt attggcgctt cgggtctggt ggcgcaaaac
     3361 tttgcctgga agctgatgaa gattggcttc aatgctgccg cagtgcgcga tatgcatgcg
     3421 ctactcgcta ccgtacaggc gtcgtcccct gacgatctgt tattagccat ttcttacacc
     3481 ggtgtacgac gcgagttaaa cctggcggca gatgagatgc tgcgagtggg cggaaaagtg
     3541 ctggcgatta ccggctttac tccgaatgcc ctgcaacagc gtgcttctca ttgcctgtat
     3601 accattgccg aagaacaagc gacaaacagt gcttcaatct ctgcttgtca cgcgcaggga
     3661 atgttaacgg atttgctgtt cattgcgctg attcagcagg atctggaact ggctccagaa
     3721 cgtattcgtc acagtgaagc gctggtgaaa aaactggtct gagtaaagaa tgcgcgtata
     3781 atgcccgccc ggtttgtgtt gttttgagag tttccttatg gcgctgttaa tcactaaaaa
     3841 atgcatcaat tgtgatatgt gtgaacccga atgcccgaat gaggcgattt caatgggcga
     3901 tcatatctat gagattaaca gcgataagtg taccgaatgc gtagggcact acgagacacc
     3961 aacctgccag aaggtgtgcc cgatccccaa tactattgtg aaggatccgc aacatgtcga
     4021 gacggaagaa cagttgtggg ataaatttgt gctgatgcat cacgcggata aaatttaata
     4081 ttctgctcct ggagtagact attgttgact ttatagtgtg agccttcagg ttcttatatt
     4141 tattttcgtg atgtgatcat aaatatcaat tatttcatga atgataacac tatctgtata
     4201 agcacttatt tataattatt aaaggcgcgc ctggctggtt aacctgacgc gccttaagta
     4261 cgtttcctta tgttataaga acagaaggat cagctgtaaa acagcaatga tgattttgat
     4321 gacccgttta ataaggtatc ggcaatcagt cattcgtttt tccttaaaca aggaatgcag
     4381 ccgttcagat tagcccttac atctccccaa aactgaacgt gcgagttatt gagggtgcat
     4441 gctgcactcc acaccagagc tttgacgaca ccactcgttt caatggggga attctgtggc
     4501 atggtataaa gcacagcaaa atcgccaata acgaagccaa ttttaatgta cttccgaatt
     4561 ggcggtcacg ttaatctttc catcaatatt gttttcttcg taaaggctcg agtttttatg
     4621 ctaaagattg caagttgctt gtaaaagata accgccctga tccataatcg ctcttgttga
     4681 gggtgcgtgc tgcacaaaat taaagttgta aagtaaaccc cctgttcctt accagttcgg
     4741 gggttttact ttttaaagag aacggtatta ttttaacttt caataattac cgtggcgcaa
     4801 gcatagtggc gttcatctgc cagcgttaca tgcatattta caacgccgag cttttccgcc
     4861 agttttaatg cctcgcccca cagtcgcagc cgtggcttac caagctcatc gttaaatact
     4921 tcaaactgat taaacgccag accattgcgg atcccggtgc caaacgcttt tgctgcggct
     4981 tcttttacgg caaagcgttt cgccagaaaa cgcaccggct ggtggtgcgt tttccaaatt
     5041 tcccattcgt tatcgcttaa tacgcggcgt gccaggcgct caccggatcg ggtgatcacc
     5101 gcttcaatgc gagcgatctc cacaatatcc gtacctaaac ctaatattgc cattagccac
     5161 gcgcttccag catcaaacgc ttcatttctg ccaccgcatc tttcagtccg gtcatcactg
     5221 cacgaccaat aatggcatga ccgatattca gttcatgcat ctcagggatg gcggcaatag
     5281 ctttcacgtt gtgataggtc agaccgtgac cagcgttaac tttcagaccg agactagcgg
     5341 caaaggtcgc ggctttggcg atacgcgcca gctcttgcgc ctgttcggcg tcagttttgg
     5401 catcagcata gcaaccggtg tggatctcga taaacggtgc gccaacctct gccgcagctt
     5461 tgatctgccc ttcatcggcg tcgataaaca gagaaacctg gatcccggcc tccgccagac
     5521 gtttacaggc atcgcgcatt ttgtcacgct gccctgcgac gtccaggccg ccttcggttg
     5581 ttacttcctg acgcttttcc ggtaccagac agcaaaaatg tggcttcgtc tcaacggcga
     5641 tcgccagcat ctcttcggtc accgccatct ccagattcat gcgggtatcc agcgtctgac
     5701 gcaggatgcg cacatcacgg tcagtaatgt ggcggcgatc ttcacgtaaa tgcacggtaa
     5761 tgccgtccgc tcccgcctgc tcagcaataa acgccgcctg caccggatcc gggtaagcag
     5821 taccgcgtgc gttacgcagc gttgcaatat ggtcaatgtt gacgcctaac agtaattcag
     5881 ccatgacaat cctcatcatt cataatgtgt tttcaccgtt cgcttaggca taaactgccg
     5941 gaacagttcc ctgctcttta atggtttacc gccaagatac ggcttaagcg ccatgcgggt
     6001 aaagcgtttc gcggcgcgca gtgtgtctgc gtcaggaaat tcccgcgcgt ttaacgcttt
     6061 taactgcctt ccggtgaacg ttttattgtc gataacgacg cttgcgataa accctttttc
     6121 ttcgcgataa cgatacgtca tggtgtcatc taccggctcg ccgctacccg cacaatgggt
     6181 aaaattgacg ccatagccca gatgcccgag cagtgccagt tcaaagcggc gcagcgcggg
     6241 ttctggcgta ccagtggcac ctgcaagaga ctgaatgcag tgcaagtaat cgaaaaagag
     6301 ttcagagaag cgcgtctcgt attccagtac gcgggagaga agttcgttga tgtacagacc
     6361 gctgtaaagc gtgataccgc ttaatggcag cgccagcgag acggcttcag cactgcgcag
     6421 cgttttgact tcgccacgcc cgccgaaacg tagcaagaga ggggtgaaag gctgtaatgc
     6481 acctttcaga gtagagcgtt tagagcgtgc gcctttggca accagacgca cgcgacccga
     6541 ttcctccgtg aagacgtcca gcatcaggct ggtttcgctc cacgggcgac tatgcaggac
     6601 aaatgcgcgc tgccagcctt ccatcggagt tactcttaaa gatcgtcaac gtaaccgaga
     6661 ctgcgcagtg cgcgttcgtc gtcggcccaa ccggatttca cttttaccca cagctcaagg
     6721 tgaacaggcg cttcgaacat ttcctgcatg tctttacgcg cttcaatccc gatggttttg
     6781 atcttggccc ctttgttgcc aataaccatc ttcttctgcc cttcacgctc aacgagaatc
     6841 aaaccgttga tgtcataacc gccgcgttcg ttagagacga aacgttcgat ctccacggtc
     6901 acggagtacg gcagttcagc gccgaggaaa cgcatcagtt tttcgcggat gatttcagac
     6961 gccataaaac gctgtgagcg atcggtgatg taatcttccg ggaagtgatg agtcgcttca
     7021 ggcagatgct tacgcacgat tgccgcaata gtatcaacat tcaacccggt ttcggcagag
     7081 atcggcacga tatcgaggaa gttcatctgg cttgccagga actgtaagtg tggcagcaga
     7141 tcggcttttt cctgcacgtt atccactttg ttcaccgcga ggattaccgg cgctttgcca
     7201 tcgcgcagtt tgttgagcac catttcatcg tccggcgtcc agcgggtgcc ttctacgaca
     7261 aaaatcacca gctcaacgtc gccgatagag ctgctcgccg ctttgttcat caggcggtta
     7321 atggcgcgtt tttcttccat atgcaggcct ggggtatcaa cgtagatcgc ctgatacgcg
     7381 ccttcagtat ggatccccac aatgcggtga cgagttgttt gcgccttgcg ggaggtaatg
     7441 gagatcttct gccccagcag tttgttcaac aatgtggatt tgccaacgtt cggacgtccg
     7501 acgatagcaa taaatccgca gtaactttta tcgatgctca ttccagctcc agttttttca
     7561 acgcctgttc ggcggcagcc tgctcagcct tacgacggct tgaacctgtg ccaaccaccg
     7621 gttcactcag gccgctgacc tggcagtgga tagtaaattc ctgatcgtgt gcttcgccac
     7681 gtacctggac gaccagataa gtcggcagcg gcagatggcg accctgcaaa tattcttgca
     7741 agcgcgtttt cggatctttt tgtttgtcac ctgggctaat ttcgtccaga cgggtttgat
     7801 accagttaag gattaatttc tcgacggttt gaatatcact gtcgaggaat acgccaccaa
     7861 ttaatgcttc gacggtgtcg gcgagaattg actcacgacg aaatccaccg cttttaagtt
     7921 cacctggccc taaacgtaag cactcgccta actcaaattc gcgcgccagt tctgccagcg
     7981 tattgccacg gaccagcgtg gcgcgcatcc ggctcatatc gccttcatcc acacgaggga
     8041 aacggtgata aagcgcattg gcgataacgt agctcagaat agagtcgcct aaaaattcta
     8101 aacgctcgtt atgtttgctg ctggcactac gatgggttaa tgcctgctgc aacagttcct
     8161 gatgattaaa agtgtagccc agcttccgtt gaagccgatt aattacgatg gggttcatgc
     8221 gataccaata aataaatgcg tcaacaattc agcacacgaa acagacctga tatacatggt
     8281 tctgctaact gcttcgctgc agtttcagtt taccggtata tggggaccaa cgctgtttcg
     8341 tgtgccgtgg caacctggag gtgccaacct taaacttcgg gggaatattc tatacacaac
     8401 gacgggggat gtcgttagcc acgggagatt tatctcataa ataattcacg ttgtcgccat
     8461 aacggcgaca acgtgaacga agatgggcta ttaatggatg ccgccaatgc gacttaagcg
     8521 cacacctgtc ggccattcgc cttcttgctt atcgaagctc atccagatag ccgttgcccg
     8581 accgaccaga ttcgcttccg gcacaaagcc ccagtaacgg ctgtccgcgc tgttgtcgcg
     8641 gttgtcaccc atcatgaagt attgtcccgg cggaacaatc caggttgcca gttgttgccc
     8701 tggctgctgg taatacatcc ccacctggtc ctgcgcaatc ggcactgtca gaatgcggtg
     8761 cgtcacatca cccagtgtct ctttacgctc ggaaagacga attccatttt ctttggtttc
     8821 gtttttcggc acttcaaaga atccgctggt cgcttcccca ccattacggc gtgagaaggt
     8881 ctgaacgaaa tcgctcggtt ccacgtttga gtaggtgacc ggcagcgcgt tttcacatgc
     8941 ctggccggaa ctacatcccg gttgaatcgt cagctctttt gagaccggat cataagtgac
     9001 tttatcgccc ggtaaaccca ccgcgcgctt gatgtaatca agctttggat cttccggata
     9061 tttaaagacc acgatatcgc cgcgtttcgg atgaccggtt tcgatcagcg ttttctggta
     9121 gataggatct ttaatgccat aagcaaactt ctctaccaga ataaaatcac cgattaacag
     9181 agtcggcatc atcgaacctg acgggatctg gaacggttca taaataaacg aacgcacaat
     9241 caatacgata gccagtaccg gaaaaacaga agcaccggtt tccagccagc caggcttcgg
     9301 cgcaaccttt ttcaacgttg ctttatccag tgagtcaccg gcagccgcct gcgccgctgc
     9361 ctgacgttcc cgccgtttag gtgcgaaaaa gaatttatcc acgcaccata aaatgcccgt
     9421 caccagtgtg gcaatcacca gaatcagggc aaacatattc gccatgccaa ctcctaaggg
     9481 ttatttgttg tctttgccga cgtgcagaat ggcgaggaac gcttcctgcg gcagctcgac
     9541 gttaccgatc tgcttcatgc gtttcttacc ttctttctgc ttctgcagca gctttttctt
     9601 acggctgata tcgccgccat aacatttagc cagtacgttt ttacgcagct gtttcacggt
     9661 ggatcgcgca ataatgtgcg taccaatcgc tgcctgaatg gcgatatcaa actgctggcg
     9721 tgggatcaga tctttcatct tctccaccaa ctcgcgaccg cggttttgcg aattatcacg
     9781 gtgggtgatc aacgccagcg catcaacacg ttcaccgttg attaatacgt ctacacgtac
     9841 catgtcggac gcctggaagc gtttgaagtt gtaatccaga gacgcataac cacgcgaggt
     9901 agatttcagg cgatcgaaga agtcgagcac cacttccgcc atcgggatct cgtacgtcag
     9961 cgccacctga ttaccgtggt aaaccatatt ggtctgcacg ccgcgttttt ctacgcacag
    10021 cgtaataacg ttgccgagat atgcctgcgg cagcagcatg tgacattctg caatcggctc
    10081 gcgcagttcg tagatgttat ttaccgctgg cagcttggat gggctgtcga cgtagataac
    10141 ttctcttgac gtggtttcaa cttcatacac tacggtcggc gcagtggtga tcagatccag
    10201 atcgtattca cgttccagac gttcctggat gatctccatg tgcagcaggc cgaggaagcc
    10261 gcagcggaaa ccaaagccca gcgcactgga gctttccggc tcatagaaca gtgaagcatc
    10321 gttcaggctg agtttgccca gcgcgtcacg gaaggcttca tagtcgtcgg aacttaccgg
    10381 gaacagaccg gcgtatacct gcggtttgac tttcttaaag ccaggcagcg ccttttctgc
    10441 cggattacgc gccagcgtta aggtatcgcc tactggagcg ccgtggatat ctttaatcgc
    10501 acataccagc cagcctactt cgccacattt cagttcagtg cggtcaacct gcttcggcgt
    10561 gaagatgccc agacggtcgg cgttataggt ctgcccggta ctcatgactt tcactttgtc
    10621 gcccttacgc agggtgccgt ttttaatacg gataagtgaa acaacgccca ggtagttgtc
    10681 gaaccaggag tcgataatga gtgcctgtaa cgggccttcc ggatcgcctt ccggcggcgg
    10741 aatgtcgcgc accagacgtt cgagaacgtc ctgcacgcca acgccggttt tcgctgaaca
    10801 gcgcaccgcg tcggtggcat cgatgccgac aatatcttca atttcttccg ccacgcgttc
    10861 aggatcggct gccggcaggt caatcttgtt cagtaccggc accacttcga gatccatttc
    10921 catggcggtg tagcagtttg ccagggtttg cgcttctacg ccctgcccgg cgtcgaccac
    10981 cagcaatgca ccttcacagg cagccagcga acgagaaact tcataagaga agtcaacgtg
    11041 gcccggggtg tcgataaagt taagctgata ggtttcgccg tcagacgctt tgtagtccag
    11101 cgtcacgctt tgcgctttga tggtaatgcc acgctcacgc tcaagatcca tggaatcgag
    11161 aacctgcgcc tccatttcac ggtcagacag gccaccgcag atctggataa tacggtcaga
    11221 cagcgtcgat ttaccgtggt caatgtgagc tatgatcgaa aagttacgta tattcttcat
    11281 aaagtatgat tattgtgcct taatgcccgg ttaaccaggc ttttagaagt cgctgttctg
    11341 agcttaacgt ctgtattaat agaaacgccg cattctacac tacaacattg aggcgaggaa
    11401 atgttcatac cgtatggatt gtggtatctg gaaacgtcct cgcatttgtt atgcaaaatg
    11461 caacaaagcc agtgaaatca ctggctcgcg tcttccgaag atgtttcaaa tcgcacaagg
    11521 ccaggcggca aggccacgct taagatgatc ggttgccatt cagaccgggc agcaaacttt
    11581 cgagagcagc cacgtgcaat caggaatcca ccaacgccac cgagaatcgc accgcataat
    11641 gctgcaatat ctgaagcaaa gagtagctga aatagtgaag cgatgaggaa tagtcccatc
    11701 aacggcgaca tataaaccag taatgcggag ctaagcaggc tgccttcggc gattcctaat
    11761 tctacttttt gccccggcac taacggctca tcacagggta cgacaatggt atgcgtggtt
    11821 tgcgggccaa gtttattcaa cacgcggcta ccgcaaccgg cgcgtgaagc gcagctgctg
    11881 catgaggctt taacatcaca actaaccagc gcctgcccgt tttcccagga gatgacggta
    11941 gcccactctt tgatcattgc gctgccccga acttaatatt ctcggcaatg cgtttcgccg
    12001 tttgcggcgg cagttcaccg acaatggtga tttcggcgtt atcacgtacg cttgtactga
    12061 cggttctgcg tccggtgcgc aacatctgat cggtgctcga tggcgtagcg cggttaacgt
    12121 ttaccgaaaa gctgaataat ccgtcggaat agagacgtga ttcgataggc atgttgtcca
    12181 tcgtcggtag cggacgtcga ctactggaaa cttcgctaaa accctgtggc aaccaggttg
    12241 gcgtccagct gaatttagct ttttcaccta caggaacaga aagcaacggc ggcaaatttg
    12301 ccttcgccaa cgtctgcata ctgctgctga tatcctgatt gacattaaaa gcaatcacgc
    12361 gaaattgttc cagcgtttca ccatcgcgat caaggagatc aacccgcatc ggtaatttcg
    12421 attcggtgtc catccacacg atgtagctgt agcgtgtacc atctcgggca accacacgaa
    12481 tgacttcgca aagacgatca gcaatacgcg tgcgcccgac ggagataaag tcgtagtaag
    12541 gagaaaggcg tttgaaatcg gtatagatga gcgatggtag agaatcaaca atgtaatcgc
    12601 cattaagcgt gaacggttca agtcccggtt caaaatagct gatttcattg ccgcgctgta
    12661 ccacttcccg gcgcgggcca tccatttgca acaattgtgc aagaggacgg ttatcgaggc
    12721 gtgcatgtcg ataacgcaga gactcaacac cctgtttatt gatgctgatg aatgacagct
    12781 cgtaattcag tgactgactg gccaggttca tctgctgtaa taacgccccg gacgcgggag
    12841 tggccgaggc gttagcagag aataacaggc tacctgtcac taatgacatg gcaaaccaaa
    12901 gttgcttcat tactgcgatt gcgttcctaa agtttgaatt cctggcacct gtacagcggc
    12961 ttgctgagtt tgcgcctgct caaactgaag ctgttcagag tggagtcggc gttgcagttc
    13021 gtaatcctgc aacattgcat taatgcgacg acgctgctcc tgtacctgct gctgttgacc
    13081 attatttgcg gtcgcttcag aaggtactcc caagctaacc gggctggctt tacccatcat
    13141 cggcagtgta ttaaataccg gcgtttcggg ctgctgggac gtttcagatt gtccattata
    13201 gtgctggacc ccaacgataa ctgcaagcga tacgcatgct gctacgccca tttgggtaag
    13261 ctgtgccgcc cacggacgta ctttctgcca gaatggcatt ttctgccatt gatgcggcgc
    13321 aggctgggct tccgggatca atgtcgccgg ttgacgtact ggctcttctt caatggcggc
    13381 catcacgcgt gaagagatat cgaaatggag cacctcggga gtatcacccc gcattgagtc
    13441 acggattaag tgatagcttt cccaggtttt ctgcatttct gggttatgag ccagttcgtt
    13501 aagcagctca ctatccagcg tttcgccatc cattaaagcg gaaagttgtt ctttctgcat
    13561 gcctaatacc cttatccagt atcccgctat cgtcaacgcc tgataagcgg ttgaactttg
    13621 ttatcaatag cttccctcgc tcggaagata cgtgaacgca ccgtacctac cggacaatcc
    13681 atgatagcgg ctatctcttc atagctcagg ccatccagct cccgcaaggt tattgccatg
    13741 cgtaaatctt ccgggaggga ctcaatagtt cggaaaacta tctgtctcag ttcttctgac
    13801 aacattaagt tctcagggtt cgaaatttct ttcaacgcgc cgccactttc gaagttttca
    13861 gcttcaatgg catccacatc actggaaggt ggacgacgcc cctgagcaac caggtaattt
    13921 ttcgctgtat ttacagcaat ccgatacagc catgtataaa aagcgctatc tccccggaac
    13981 gaatccagcg cacgataggc tttaataaaa gcttcttgta ccacatcggg aacatcaccc
    14041 gacggcacat agcgggaaac cagactcgcc actttatgct gatagcgcac taccagtaag
    14101 ttaaaggctt tctgatctcc cttctggacc cgttcaacca ggacctggtc cgttaactgc
    14161 tcgctcatcc gaggtaaagt ctccccaaac caaatttcca cgcgctatcg aaacgccact
    14221 ccattagctg caatttgagc aagcaaaggg ttagagtgtc tcgtttttgt aaagttccgt
    14281 aacgcatctg tttttgtttg tcatgctgta gacggatcat tatctatcat tataagtcta
    14341 cagaatctga acatcgcatt atctgtgtag aaatgcccat ttaactgcct gaagagtaac
    14401 ccaacggcct ttttatttca ccacctaatc ctccaccagc cagtaacttc tctttttctc
    14461 gccgccctgc gtcagcgtgt ttagccactg taacaaatat taaaatagca ggtgtttaat
    14521 cgcacaacat gatgctatgc tgaccaaacc gtgtttagta aattaaacaa agaaaatgaa
    14581 tactctccct gatcattcat gtgacgtgtt gattatcggt agcggcgcag ccggactttc
    14641 actggcgctg cgcctggctg atcagcatca ggtcatcgtt ctaagtaaag gcccggtaac
    14701 ggaaggttca acattttatg cccaaggcgg tattgccgcc gtgtttgatg aaactgacag
    14761 cattgactcg catgtggaag acacattgat tgccgggggt ggtatttgcg atcgccatgc
    14821 agttgaattt gtcgccagca atgcacgatc ctgtgtgcaa tggctaatcg accagggggt
    14881 gttgtttgat acccacattc agccaaatgg cgcagaaagc taccatctga cccgtgaagg
    14941 tggacatagt caccgtcgta ttcttcatgc cgccgacgcc accggtagag aagtagaaac
    15001 cacgctggtg agcaaggcgc tgaaccatcc gaatatttgc gtgctggagc gcagcaacgc
    15061 ggttgatcta attgtctctg acaaaattgg cctgccgggc acgcgacgag ttgtaggcgc
    15121 gtgggtatgg aaccgaaata aagaaacggt tgaaacctgc cacgcaaaag cggtggtgct
    15181 ggcaaccggc ggtgcgtcga aggtttatca gtacaccacc aatccggata tttcttctgg
    15241 cgatggcatt gctatggcgt ggcgcgcagg ctgccgggtt gccaatctcg aatttaatca
    15301 gttccaccct accgcgctgt atcacccaca ggcacgcaat ttcctgttaa cagaagcact
    15361 gcgcggcgaa ggcgcttatc tcaagcgccc ggacggtacg cgttttatgc ccgattttga
    15421 tgagcgcggc gaactggccc cgcgcgatat tgtcgcccgc gccattgacc atgaaatgaa
    15481 acgcctcggc gcggattgta tgtatctcga catcagccat aagcctgccg attttattcg
    15541 ccagcatttc ccgatgatct atgaaaaatt gctcgggctg gggattgatc tcacacaaga
    15601 accggtaccg attgtgcctg ctgcacacta tacctgcggt ggtgtgatgg tggatgatca
    15661 tgggcgtacg gacgtcgagg gcttgtatgc cattggcgag gtgagttata ccggcttaca
    15721 cggcgctaac cgcatggcct cgaattcatt gctggagtgt ctggtctatg gctggtcggc
    15781 ggcagaagat atcaccagac gtatgcctta tgcccacggc gtcagtacgt taccaccgtg
    15841 ggatgaaagc cgcgttgaga accctgacga acgggtagta attcagcata actggcacga
    15901 gctacgcctg tttatgtggg attacgtcgg cattgtgcgc acaacgaagc gccttgaacg
    15961 cgccctgcgg cggataacca tgctccaaca agaaatagac gaatattacg cccatttccg
    16021 cgtctcaaat aatttgctgg agctgcgtaa tctggtacag gttgccgagt tgattgttcg
    16081 ctgtgcaatg atgcgtaaag agagtcgggg gttgcatttc acgctggatt atccggaact
    16141 gctcacccat tccggtccgt cgatcctttc ccccggcaat cattacataa acagataaaa
    16201 agcctgggtc agcgccgtat acgcttcgga atagttctgg tctggcccac gaatgactaa
    16261 gcgatcgcta aagcattctc ccgcctgcgg ggagaatgcc agcagcaccc gatgcggcag
    16321 tcgcgcttcg ttttccgcca catccgtccg caaacgtaaa tgccagccca tacttaatgc
    16381 cagttcagta aaaccattac caatctgctc tggcagcacc acgcagaaaa atccctcttc
    16441 ggtaatgcac tccgccgcac aggtcagcaa cgatgggtga tcaagcgtag tggtatagcg
    16501 agcctgttcc cgttgaggtg tcgagcactc tactccctgc tgatagtaag gtgggttact
    16561 gatgattaaa tcgaagcgta ctgtctgctg tgtgatccac tgctgaatat ccgccgtatg
    16621 gacgttaatc cgctttgccc acggggactg gttgatattt tcctgtgcct gcgcggcagc
    16681 ttcactttcc agttcaactg catcaatcat tacgctgtca tcggttcgct gcgccagcat
    16741 taatgccagc aacccgctac ccgcgccgat atcaaggcaa cgttttaccc cagccaccgg
    16801 tgcccatgcg cccagcaaaa tgccatccgt tcccactttc atcgcacaac gatcgtgagc
    16861 aacaaaaaac tgtttaaaag taaatccatt acgacgaagc acggatgtag actgtgacat
    16921 gaaaataaaa ccttgcagga aaaacggcga tagcaccggg tgagaacaat acccgagaag
    16981 cgatatccat acaaacagat gaagattgca gccgtaacgt ctataatcag cgccccacac
    17041 agaggtagaa catgactgta acgacttttt ccgaacttga actcgacgaa agcctgctgg
    17101 aagccctcca ggataaaggt ttcactcgcc cgaccgccat tcaggctgcc gccattccgc
    17161 ctgcgctcga tggccgtgat gtactcggtt ctgcgccgac aggcaccggt aaaacggcgg
    17221 cgtatctgct gccagcgttg cagcacctgc tcgatttccc acgtaagaaa tccggtccgc
    17281 cgcgtatttt gatcctcacc ccaactcgcg agctggcgat gcaggtggcc gatcatgccc
    17341 gcgaactggc gaaacatacg catctggata ttgccaccat caccggcggc gtagcctata
    17401 tgaaccacgc ggaagtgttc agcgaaaatc aggacatcgt ggtcgccacg accggacgtc
    17461 tgctgcaata cataaaagaa gagaacttcg attgccgcgc ggttgaaacg ctgatcctcg
    17521 acgaagcaga ccgtatgctg gatatgggct tcgctcagga tatcgaacat attgctggcg
    17581 aaacgcgctg gcgtaaacag accctgctct tttcggcaac tctggaaggc gatgcgattc
    17641 aggactttgc cgagcgtctg ctggaagatc cggtggaggt ttctgccaat ccgtccacac
    17701 gtgagcgcaa aaaaattcat cagtggtatt accgcgccga tgacctggaa cataaaaccg
    17761 cattgctggt gcatctgtta aaacagccgg aagcgacccg ctcaattgtg tttgtgcgta
    17821 agcgtgagcg tgtgcatgag ctggcaaact ggctgcgcga agcgggcatc aacaactgct
    17881 atctcgaagg tgagatggta cagggcaagc gtaacgaagc gatcaagcgt ttgaccgaag
    17941 gtcgcgtaaa cgtactggtt gccaccgatg ttgccgcgcg cggtatcgac attcctgacg
    18001 tcagccacgt ctttaacttc gatatgccgc gcagtggcga tacttatttg caccgtatcg
    18061 gacgtaccgc gcgcgccggt cgtaaaggca ccgcaatttc gctggtagaa gcccatgacc
    18121 atctgctgct gggcaaagtt ggtcgctata ttgaagagcc aattaaagct cgtgtaattg
    18181 atgagttacg cccgaaaacg cgtgcgccaa gcgagaagca gaccggcaag ccatcgaaga
    18241 aagtactggc aaaacgcgcc gagaagaaaa aagctaaaga gaaagagaag ccgcgggtga
    18301 aaaaacgcca tcgcgacacc aaaaatattg gtaagcgccg taaaccgagc ggaacgggcg
    18361 tgccaccgca aacgacagaa gagtaatctc aatgccgggt ttaagcccgg cattattttt
    18421 atcccggcaa aaaacactct tcaacacact gaataaaaat gtgcatcgcc gggctaaccg
    18481 cttttccggc atggtgagcg cacattgccg taatggtctg cgactgttcg ccaaaaggca
    18541 attcgattaa ttcgccactc gcaagttcat ttgccactgt aaagcgcggc aggtaactga
    18601 ccccgatatt tgccgcgaca caacgcttga tgctttcgat acttataagc tcaatggtgt
    18661 tttccaccgt gatccgtcgc tggcgcagcg tactttcaaa tagctggcga aagacacatt
    18721 gcggttcatt gataataaaa ctacaggcgt tatgtcttcc cggctcagta aaatcgacat
    18781 ctttaatttg cggtgaagcc accagcacca gtgattgttc acccaactct cgtcggttca
    18841 gagcatcatc attccctacg cggtaaaaga cgccgacatc agcctcatca ttcagtagcg
    18901 catcacggat cacgtaacag ttcagagact gcaacgataa acgcactttt ggtgcgcgtt
    18961 gccgaaaacg ctgcaacacc tgcggcattc ggtaagaaag caacgtttcg cctgaaacaa
    19021 cgcgtagttc cccgtccgga tccgactctt ttttggcagc ctcacgaagg gtatccatca
    19081 ctctggtgag ttcatagatg tgcggcagca gcttttttcc ttcgcgggta aggcacattc
    19141 gtcgaccaat tttctcaaat aactggactg agaactcctg ctcaagctgc tgaatatgaa
    19201 aagtcaccgt cgattgtgtg cagcacaatt tttgcgaagc tcgcaaaaag gacccctctt
    19261 ccaccacggt tttaagcgta ataaaacggc gcagatccat aaccccaaac ctatcgaaaa
    19321 tatcgaattt agaatgtaaa aacattcatt tttttaaatg ttccgtgtcg ggtactgtct
    19381 accaaaacag aggagataac aagtgacacc gaccctttta agtgcttttt ggacttacac
    19441 cctgattacc gccatgacgc caggaccgaa caatattctc gcccttagct cagctacaac
    19501 gcacggattt cgtcagagta ctcgcgtgct ggcagggatg agtctgggat ttttaatcgt
    19561 catgttgcta tgcgtgggaa tttcgttttc actggctgtt atcgacccgg cagcagtgca
    19621 tctgttgagc tgggcaggag cggcatatat tgtctggctg gcatggaaaa tcgctaccag
    19681 tccaacaaag gaagacggac ttcaggcaaa accaatcagc ttttgggcca gctttgcttt
    19741 gcagtttgtg aacgtcaaaa tcattttgta tggcgttacg gcactgtcga cgtttgtcct
    19801 gccgcaaacg caggcgctaa gctgggtagt tggcgtcagc gttttgctgg cgatgattgg
    19861 gacgtttggc aatgtgtgct gggcgctggc ggggcatctg tttcagcgat tgtttcgcca
    19921 gtatggtcgc ccgttaaata tcgtgctggc gctgttgctg gtctattgtg cggtacgcat
    19981 tttctattga aagacaaaaa gaaaagcgga agaggtcacc ctcttccgct taataacttg
    20041 ctacttaagc cttacaggct ttcagtaaag gtacgagcga taacgtcgcg ctgctgttcc
    20101 ggagtcagag agttaaagcg aactgcataa ccggatacac ggatggtcag ctgcggatat
    20161 ttttccggat gcttaactgc atcttccaga gtttcacgac gcagaacgtt aacgttcagg
    20221 tgttgaccac cttcaacgcg aacttctggt ttcacttcta ctggaacttc acggtattca
    20281 atatcaccca gtttgcttac tgcaaccact tcgtcttctg caaaacctgc tttcgcaacg
    20341 atgcaacgcg cttcgccttt ttcgctgtcc agcagccaga aagagttcag cagatcgtcg
    20401 ttagcggctt tagtaatctg gatacctgta atcatgtgat gcctccccgg caaaattatt
    20461 tgatttgttc agcctgtcgc ggccaattgg taaaaccatt gttgcttgag tgtatatata
    20521 ctcctcaaac acccttgaat ctttgattta aatcaataaa aaccacacat caagtatggt
    20581 tgcaaatgga ttttattgtt ttacatcaac ttatgcaggt gtgaaatttt accaatttac
    20641 atttttttgc actcgtttaa gtctaaaaaa tgagcatgat tttgttctgt agaaagaagc
    20701 agttaagcta ggcggattga agattcgcgg gagagcgaga tggcaaacga attaacctgg
    20761 catgacgtgc tggctgaaga gaagcaacaa ccctattttc ttaataccct acagaccgtc
    20821 gccagcgagc ggcagtccgg cgtcactatc tatccgccac aaaaggatgt ctttaacgcg
    20881 ttccgcttta cagagttggg tgacgttaaa gtggtgattc tcggccagga tccttatcac
    20941 ggaccgggac aggcgcatgg tctggcattt tccgttcgcc ccggcattgc gacaccgccg
    21001 tcactgctga atatgtataa agagctggaa aatactattc ccggtttcac ccgccctaat
    21061 catggttatc ttgaaagctg ggcgcgtcag ggcgttctgc tactcaatac tgtgttgacg
    21121 gttcgcgcag gtcaggcgca ttcccacgcc agcctcggct gggagacctt caccgataaa
    21181 gtcatcagcc tgattaacca gcatcgcgaa ggcgtggtgt ttttgttgtg gggctcacat
    21241 gcgcaaaaga aaggggcgat tattgataag caacgccatc acgtactgaa agcgccgcat
    21301 ccatcaccgc tttctgcgca tcgtgggttc tttggctgca accattttgt gctggcaaat
    21361 cagtggctgg aacaacgtgg cgagaaaccg attgactgga tgccagtatt accggcagag
    21421 agtgagtaaa tttgcaggaa aacgccggat ggcagcgttg ccacccggct gatttatcag
    21481 gctttattct gacgccacca ttcaccaagc aaaacgccag ttgcgacaga gatatttagc
    21541 ccggcaacgt tgccagtacc gtcaatcttc acgcgcagat cgtttggatc gcgtgcggca
    21601 tccggtaacc cttcatattc ctgacccagc accagtacca ttttcgctgg cagactggtt
    21661 ttgaacagcg gtttcccctg ctcgctggaa gtggtcacta cggtgtaacc tgcctgacgg
    21721 aaatcatcca gcacgttaac aatgttgtcg ccagtaatcg gctgaacgtg ctctgcgccg
    21781 ccttctgcgg tacggatagc cgcccccgac tccagcagtg cctcatcctg caccacaacg
    21841 cctttcacgc cgaagtgcgc acagctgcgc atcatgccac ccaggttatg cgggttagat
    21901 tcgttttcca gtgccagaac gcaatcctgc gcgcctgcct ggcttaccca ctgctgcacg
    21961 gttgtaccgt tacgcttttt gatcaggaag caaacgccgc cgtgatgttc cgtgcctgac
    22021 gcttttgtca gttccgcttc atctaccaca tggtacgctt tgcggtttgc tgccatccag
    22081 cgcaaggctt ctttaaaacg cggcgttaca ctctggataa accaggcgcg aacaatcgct
    22141 tccggacggc tctggaacag tgcctgacag gcattttcgc cgtagacgcg ggtttcttcc
    22201 gcacgctgac gacgcaaaac ttccggatca ataaaacttt taccactgat gccaccgtga
    22261 tcggcctttt ccggcgtctc atcacccggc gcgcgggaaa ccgtgcgcca cggcgaatct
    22321 tcccacttgc ggtcacgggg gtgactctgt ttgtcatcgc gggcggggcg acggccaccg
    22381 tcagcacgag attttcctgg acgcccgccc cctttcccgg tacgcgggtt gtgggtacgt
    22441 ttatcagaat catcatcact gcggacatac atcactttga ccttgccgct tttacctttc
    22501 atttcatcgt tcatgctttt ctccaccagc gctgcgcgaa gcgcgcagat tacccgaagt
    22561 ccgcgcggtt cgccatgatt tcgtaccaaa gcctgcgact atcataccta ttgaataaaa
    22621 cagattgttg tctggaacaa tgtccccgat aatatgtaac atattagaaa cataccggcg
    22681 tcgttgccga taagtctcct tactcatccc gaggttagtt atgaataccg tttgtaccca
    22741 ttgtcaggcc atcaatcgca ttcccgacga tcggatcgaa gatgcggcaa aatgcggacg
    22801 ctgcggtcac gacttgtttg acggagaggt gattaatgcg accggtgaaa cgctcgacaa
    22861 attgctgaaa gatgatctac ctgtggtgat cgacttctgg gcaccgtggt gcggcccctg
    22921 ccgtaatttc gcaccaattt ttgaagatgt cgcgcaagag cgtagcggta aagtgcgctt
    22981 tgtgaaagtg aataccgaag ctgaacgtga attgagcagt cgctttggaa ttcgtagtat
    23041 accgacgatc atgattttca aaaacggtca ggttgtcgac atgcttaatg gcgcagtacc
    23101 gaaagcgccg tttgatagct ggctgaacga atctctttaa tcttaccggg gcgcatcttg
    23161 tgccccgttt tctcctctgc gacaatggcg ttttttcgac gctctcttat gaccgaaaac
    23221 gctgttctcc agttacgcgc cgagcgtatt gcgcgcgcaa cacgtccttt tcttgcccgc
    23281 ggtaatcgcg ttcgtcgctg ccaacgctgt cttttgccag agaaattatg tctctgttcg
    23341 acaattaccc cagcacaagc aaaaagtcgc ttctgtttgc tgatgttcga caccgagcca
    23401 atgaagccca gtaataccgg gcgtctgatt gctgatattt tgcctgatac cgttgcgttt
    23461 caatggtcgc gtaccgaacc ctcgctagat ttgctggatc tggtacaaaa cccggactat
    23521 cagccaatgg tggtctttcc cgcctcgtat gctgatgagc aacgggaagt gatcttcaca
    23581 ccacctgccg gtaagcctcc gctatttatc atgctcgatg gtacctggcc tgaagctcgc
    23641 aagatgtttc gtaaaagtcc gtatctggat aatcttcccg tcatttccgt cgatctttcc
    23701 cggctttctg cctatcgcct gcgtgaagcc caggctgaag gccaatattg tactgccgag
    23761 gtagccatcg cactgttaga tatggccggc gataccgggg cggcggcagg gttaggcgag
    23821 cattttaccc gctttaaaac acgctatctg gcaggaaaaa cgcaacatct gggtagcatc
    23881 acagcagaac agttagaaag cgtttaaaat cattcggtca cttctgcggg agaccggtat
    23941 gagtcagcga ggactggaag cactactgcg accaaaatcg attgcggtaa ttggcgcatc
    24001 gatgaaaccc aatcgcgcag gttacctgat gatgcgtaac ctgctggcgg gaggctttaa
    24061 cggaccggta ctcccggtga cgcctgcctg gaaagcggtg ttgggtgtgt tggcctggcc
    24121 ggatattgcc agcttgccct ttacacccga ccttgcggtt ttatgtacca atgccagccg
    24181 taatcttgct cttctggaag agctcggcga gaaaggctgt aaaacctgca ttattctttc
    24241 cgccccggca tcgcaacacg aagatctccg cgcctgcgcc ctgcgccata acatgcgcct
    24301 gctaggacca aacagtctgg gtttactggc tccctggcaa ggtctaaatg ccagcttttc
    24361 acctgtgccg attaaacgcg gcaaactggc gtttatttcg caatcggctg ccgtctccaa
    24421 caccatcctc gactgggcgc aacagcgtga gatgggcttt tcctacttta ttgcgctcgg
    24481 cgacagcctg gatatcgacg ttgatgaatt gctcgactat ctggcgcgcg acagtaaaac
    24541 cagcgccatc ctgctctatc tcgaacagtt aagcgacgcg cgacgctttg tttcggcggc
    24601 ccgtagtgcc tctcgcaata aaccgattct ggtgattaaa agcggacgta gcccggcggc
    24661 tcaacgtctg ttgaacacca cggcaggaat ggatccggcg tgggatgcgg ctattcagcg
    24721 tgccggtttg ttgcgggtac aggatactca cgagctgttt tcggcagtgg aaaccctcag
    24781 ccatatgcgc ccactgcgtg gcgaccggct gatgattatc agcaacggtg ctgcgcctgc
    24841 cgcgctggcg ctggatgcct tatggtcacg caatggcaag ctggcaacgc taagcgaaga
    24901 aacctgccag aaactgcgcg atgcactgcc agaacatgtg gcaatctcta acccgctcga
    24961 tctacgcgat gacgccagca gcgagcatta tgtcaaaacg ctggacattc tgctccacag
    25021 ccaggatttt gatgcgctga tggttattca ttcgcccagc gccgctgctc ccgcaacaga
    25081 aagcgcgcaa gtattaattg aagcggtaaa gcatcatccc cgcagcaagt atgtttctct
    25141 gctgacgaac tggtgtggcg aacattcctc gcaagaggca cgacgattat tcagcgaagc
    25201 cgggctgccg acctaccgta ccccggaagg aaccatcact gcttttatgc atatggtgga
    25261 gtaccggcgt aatcagaagc aactacgcga aacgccggcg ttgcccagca atctgacctc
    25321 caataccgca gaagcgcatc ttctgttgca acaggcgatt gccgaaggag caacgtcact
    25381 cgatacccat gaagttcagc ccatcctgca agcatatggc atgaacacgc tccccacctg
    25441 gattgccagc gatagcaccg aagcggtgca tattgccgaa cagattggtt atccggtggc
    25501 gctgaaattg cgttcgccgg atattccaca taaatcggaa gttcagggcg taatgctcta
    25561 cctgcgtaca gctaatgaag tccagcaagc ggcaaacgct attttcgatc gcgtaaaaat
    25621 ggcctggccg caggcgcgga tccacggcct gttggtgcaa agtatggcta accgtgctgg
    25681 cgctcaggag ttgcgggttg tggttgagca cgatccggtt ttcgggccgt tgatcatgct
    25741 gggcgaaggc ggtgtggagt ggcgtcctga agatcaagcc gtcgtcgcac taccgccgct
    25801 gaacatgaac ctggcccgct atctggttat tcaggggatc aaaagtaaaa agattcgtgc
    25861 gcgcagtgcg ctacgcccat tagatgttgc aggcttgagc cagcttctgg tgcaggtttc
    25921 taacttgatt gtcgattgcc cggaaattca gcgtctggat attcatcctt tgctggcttc
    25981 tggcagtgaa tttaccgcgc tggatgtcac gctggatatc gcgccgtttg aaggcgataa
    26041 cgagagtcgg ctggcagtgc gcccttatcc gcatcagctg gaagaatggg tagaattgaa
    26101 aaacggtgaa cgctgcttgt tccgcccgat tttgccagaa gatgagccac aacttcagca
    26161 attcatttcg cgagtcacca aagaagatct ttattaccgc tactttagcg agatcaacga
    26221 atttacccat gaagatttag ccaacatgac gcagatcgac tacgatcggg aaatggcatt
    26281 tgtagcggta cgacgtattg atcaaacgga agagatcctt ggcgtcacgc gtgcgatctc
    26341 cgaccctgat aacatcgatg ccgaatttgc cgtgctggtt cgctcggatc tcaaagggtt
    26401 aggcttaggt cgacgcttaa tggaaaagtt gattacctat acgcgagatc acggactgca
    26461 acgtctgaat ggtattacga tgccaaacaa tcgtggcatg gtggcgctgg cccgcaagct
    26521 cgggtttaac gttgatatcc agctcgaaga ggggatcgtt gggcttacgc taaatcttgc
    26581 ccagcgcgag gaatcatgag taaggtactg gaaatgttga ccacttaatc gggactggtg
    26641 ttattattgc ccgcttatgt cgtctgcatt gcacagagga cccttcaatg aacagagaag
    26701 aaatgcactg tgatgttgtc aaaatttaag cgtaataaac atcaacaaca ccttgcccaa
    26761 ctacccaaga tttctcaatc agttgatgat gtcgatttct tttacgctcc cgccgacttc
    26821 cgggagacgc tgctggaaaa aatagccagc gcgaagcagc gcatttgcat tgtcgccctg
    26881 tatctcgaac aggatgacgg tggcaaaggc attctgaacg cgttgtatga ggctaaaagg
    26941 cagcgtccgg aactggatgt gcgggtgctg gtcgactggc atcgtgcaca acgtggacgc
    27001 attggcgctg cggcatctaa cactaacgct gactggtact gccgcatggc gcaggaaaat
    27061 ccgggcgtag atgttccggt ttatggcgtt ccaatcaata cccgtgaagc ccttggtgtt
    27121 ctgcacttta aaggctttat catcgacgat agcgtacttt atagcggtgc cagcctgaac
    27181 gatgtttacc tgcatcagca cgataaatat cgctacgacc gttatcatct tatccgtaac
    27241 cgtaagatgt cagacattat gtttgaatgg gttacacaga atattatgaa tggccgcggc
    27301 gttaatcgtc tggatgatgt taatcggcca aaaagcccgg aaatcaagaa cgatattcgt
    27361 ctgttccgcc aggagctgcg tgatgccgct tatcatttcc agggcgatgc cgacaacgat
    27421 cagctttccg taacgccgtt ggtaggtctg gggaaatcga gtctgttgaa caagaccatt
    27481 ttccatctta tgccttgtgc cgagcagaaa ctaaccatct gcacacctta cttcaacctg
    27541 ccagcaatcc ttgtgcgcaa tattatccag ttgctgcgcg aagggaaaaa ggtcgaaatt
    27601 attgttggtg ataaaaccgc gaatgacttc tacattccgg aagatgaacc tttcaagata
    27661 attggcgcat tgccttatct ctatgagatc aatctgcgtc gtttcctgag ccgtttgcag
    27721 tattacgtca acactgacca gttagtggtt cggttatgga aagatgacga caacacctat
    27781 cacctgaaag ggatgtgggt tgatgataag tggatgttga tcactggtaa taacctgaac
    27841 ccgcgcgcct ggcgtctgga tctggaaaac gctattttga tccacgatcc gcaactggag
    27901 cttgcgccac agcgagagaa agaactggag ctgatccgcg agcataccac catcgttaag
    27961 cactatcgcg acctgcaaag tattgccgat tatccggtga aggttcgtaa actcatccgt
    28021 cgtttgcgcc gtatccgcat cgaccgatta attagccgca tcctgtaatc acaaccccgt
    28081 cctgtacggg gtttgttttt tggaggccac gttttgcgta ttctttttgt ctgttcgcta
    28141 ttattacttt ctggatgcag ccatatggct aacgatagct ggagcgggca ggataaagct
    28201 caacacttta tcgcctcggc gatgctttcc gccgccggaa atgaatatgc acagcatcag
    28261 gggatgagtc gcgatcgcag tgccactttc gggctgatgt tctctgttgg gctgggggcg
    28321 tcaaaagaac tttgggatag ccgccccgag gggagcggct ggagctggaa agattttacc
    28381 tgggatgtcg ccggtgcaac tactggctat accgtctggc aactgacccg tcactaaaga
    28441 cgcatcccct tccctttgcg atgtagcatc aaagaaacca gaaacgccac cacggccatc
    28501 aaggtcacat accagaagaa ggctgtttcc attcctattg atttcagcga caacgctacg
    28561 tactccgccg aaccaccaaa tatagcattg gcgaccgcat atgacagacc aacacctaat
    28621 gcacgaacct gtgccgggaa catctcagcc ttcagtattc cactgattga agtataaaaa
    28681 ctcacgatca gcagggcaca catcaccaga ccaaaagcgg cataaggcga ggaaacgttt
    28741 tgcaatgctg agagaatagg aacggtaaaa atggctgcca gagaaccgaa acataacatt
    28801 gaggtacgac gaccaatctt atccgacagc gcgccaatga gtggttgaat aagcatgaat
    28861 acaaacaatg cggcagtcat aatgccactc gccacgttgg catgcattcc cgcagtattt
    28921 accagatact tctgcatata agtggtgaag gtatagaaac aaagggagcc cgcagcggta
    28981 aaaccgagaa ccatgatgaa tgcacggcga ttgcgccata atcctttcag agatccagct
    29041 tcttttaaag cgcgtgtttc ttgttgtgaa gtttcatcta actgacgacg taaccacaac
    29101 gccacaacag ctaacacagc tcctaacgcg aaaggaatac gccatcccca ctctctgagt
    29161 gcagcgtctt ccatggtgtg ttgtaaaacc acgacaacca gtagagctag cagttgcccg
    29221 ccgatcaacg tcacatactg aaatgatgcg taaaaacctt tgcgcccttc aacggcaact
    29281 tcactcatat aggtggcgct ggtgccatat tctccgccaa cggataatcc ctggaataaa
    29341 cgagcaagaa gcaataatgc cggagcccag gtaccgattg tttcgtagcc tggcaaacag
    29401 gcgataacca gtgagccgaa acacatcata cacaccgata acagcatcga ttttttgcga
    29461 ccatgtttat cggctatgcg gccaaatagc caaccgccta ttgggcgcat cagaaatccc
    29521 gcagcaaaaa cacctgctgt ttgcagtagt tgagtcgtcg tgttcccgga agggaagaag
    29581 atgtgagcga agtagagtga acagaacgag tagacataga aatcgaacca ctcgaccaga
    29641 ttacctgaag aggcccccac aattgcccaa atgcggcgac gagtatcact acttgtcagt
    29701 ttgctgtctg ccgttacagt actttcagcc atgccattat gtctcctgcc gtaatccgat
    29761 gcttttgtcg gtcgcttttg tttatttctt ttggtaaaga aaatgttata catttgttgc
    29821 atatcatcat gcaaccttaa ccacgaattt agttagcagg aaaatggtta ttgaggagct
    29881 taaggataaa tttctggtaa ggaggacacg tatggaggtg ggcaagttgg ggaagccgta
    29941 ttcgctggcg tatgtggagg aataagctgc cccctatcgt gcctgcgtaa gctggataag
    30001 acgcgccatg aactgaaccc caaaacctgg acagtttcag ttaaccggct ttttgatact
    30061 cagtccggta ttctaccggg ctgagtcccc caagactgag ttttattctt ttttgattcc
    30121 agtaagttat gtattcgtta acggcatttt ccagctcttc taca
//
LOCUS       DACBVI010000044        30001 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.044, whole genome shotgun sequence.
ACCESSION   DACBVI010000044 DACBVI010000000
VERSION     DACBVI010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.044"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            112..1908
                     /locus_tag="IU325_09550"
     CDS             112..1908
                     /locus_tag="IU325_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002954510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HAP5809272.1"
                     /translation="MFDKYRKTLVAGAVAITLGLSAPGVMAAGFKPAPPAGQLGAVIV
                     DPYGNAPLTALVDLDSHVISDVKVTVHGKGEKGVEISYPVGQESLKTYDGVPIFGLYQ
                     KFANKVTVEWKENGKVMKDDYVVHTSAIVNNYMDNRSISDLQQTKVIKVAPGFEDRLY
                     LVNTHTFTAQGSDLHWHGEKDKNAGILDAGPATGALPFDIAPFTFIVDTKGEYRWWLD
                     QDTFYDGRDRDINKRGYLMGIRETPRGTFTAVQGQHWYEFDMMGQVLEDHKLPRGFAD
                     ATHESIETPNGTVLLRVGKSNYRRDDGVHVTTIRDHILEVDKSGRVVDVWDLTKILDP
                     KRDALLGALDAGAVCVNVDLAHAGQQAKLEPDTPFGDALGVGPGRNWAHVNSIAYDAK
                     DDSIILSSRHQGVVKIGRDKQVKWILAPSKGWEKPLASKLLKPVDANGKPITCNENGL
                     CENSDFDFTYTQHTAWISSKGTLTIFDNGDGRHLEQPALPTMKYSRFVEYKIDEKKGT
                     VQQVWEYGKERGYDFYSPITSIIEYQADRNTMFGFGGSIHLFDVGQPTVGKLNEIDYK
                     TKEVKVEIDVLSDKPNQTHYRALLVRPQQMFK"
     gene            1926..2594
                     /locus_tag="IU325_09555"
     CDS             1926..2594
                     /locus_tag="IU325_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HAP5809273.1"
                     /translation="MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNA
                     DKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVL
                     INKDKAAGISLFDANSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQA
                     DFEAALKEPAVQETLEKWKASYEVAKIQGVPAYVVNGKYLIYTKSIKSIDSMADLIRE
                     LASK"
     gene            2609..3280
                     /gene="dsbI"
                     /locus_tag="IU325_09560"
     CDS             2609..3280
                     /gene="dsbI"
                     /locus_tag="IU325_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide oxidoreductase DsbI"
                     /protein_id="HAP5809274.1"
                     /translation="MGIKGMWKDLRTSPVDTLVRWQEQRFLWLLMAIAMGALIILAHS
                     FFQIYLYMAPCEQCVYIRYAMFVMVIGGLVAAINPKNIILKLIGCVMAFYGSILGLKF
                     SLKLNDIHHAVHNPDPDSLFGVQGCSTDPTFPFNLPLAQWAPNWFKPTGDCGYDAPIV
                     PDGVTLSSTQQWFVEMYQQSEGWYLLPPWHFMNMAQACMLAFGMCLVLLVIMSGAWAL
                     KIIRG"
     gene            complement(3326..4141)
                     /gene="ygiD"
                     /locus_tag="IU325_09565"
     CDS             complement(3326..4141)
                     /gene="ygiD"
                     /locus_tag="IU325_09565"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HAP5809275.1"
                     /translation="MTPLVKDIIMSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMT
                     LPRPQAIVVVSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQHLV
                     ELLAPVPVELDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLA
                     ALRDEGIMLVASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVN
                     YLDHEGGALSNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            4257..5030
                     /gene="zupT"
                     /locus_tag="IU325_09570"
     CDS             4257..5030
                     /gene="zupT"
                     /locus_tag="IU325_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HAP5809276.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(5088..5258)
                     /locus_tag="IU325_09575"
     CDS             complement(5088..5258)
                     /locus_tag="IU325_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809277.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(5520..6173)
                     /gene="ribB"
                     /locus_tag="IU325_09580"
     CDS             complement(5520..6173)
                     /gene="ribB"
                     /locus_tag="IU325_09580"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HAP5809278.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRIMTVRAAIADDAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(6277..6425)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6547..6846
                     /locus_tag="IU325_09585"
     CDS             6547..6846
                     /locus_tag="IU325_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HAP5809279.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNSVADLQSPPAIPPIDKAE"
     gene            complement(6881..7081)
                     /gene="glgS"
                     /locus_tag="IU325_09590"
     CDS             complement(6881..7081)
                     /gene="glgS"
                     /locus_tag="IU325_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HAP5809280.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            7351..7980
                     /locus_tag="IU325_09595"
     CDS             7351..7980
                     /locus_tag="IU325_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HAP5809281.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMISGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACLMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            8007..9668
                     /locus_tag="IU325_09600"
     CDS             8007..9668
                     /locus_tag="IU325_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin"
                     /protein_id="HAP5809282.1"
                     /translation="MDDIVNSVPSWMFTAIISVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQSLPAVIEKSVEPMKSIDGIKIIQVDGLNRGGAAGNAASGSVSGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            9907..9966
                     /locus_tag="IU325_09605"
     CDS             9907..9966
                     /locus_tag="IU325_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAP5809283.1"
                     /translation="MMKLVIILIVLLLVSFATY"
     gene            10283..10342
                     /gene="ibsE"
                     /locus_tag="IU325_09610"
     CDS             10283..10342
                     /gene="ibsE"
                     /locus_tag="IU325_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HAP5809284.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(10462..11895)
                     /gene="hldE"
                     /locus_tag="IU325_09615"
     CDS             complement(10462..11895)
                     /gene="hldE"
                     /locus_tag="IU325_09615"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HAP5809285.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(11943..14783)
                     /gene="glnE"
                     /locus_tag="IU325_09620"
     CDS             complement(11943..14783)
                     /gene="glnE"
                     /locus_tag="IU325_09620"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HAP5809286.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWQQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDCKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTAMDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            complement(14806..16107)
                     /locus_tag="IU325_09625"
     CDS             complement(14806..16107)
                     /locus_tag="IU325_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP5809287.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTKTAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDINSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            16349..16969
                     /locus_tag="IU325_09630"
     CDS             16349..16969
                     /locus_tag="IU325_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAP5809288.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            17033..18271
                     /gene="cca"
                     /locus_tag="IU325_09635"
     CDS             17033..18271
                     /gene="cca"
                     /locus_tag="IU325_09635"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HAP5809289.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLALM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(18352..19173)
                     /locus_tag="IU325_09640"
     CDS             complement(18352..19173)
                     /locus_tag="IU325_09640"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP5809290.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFILAAAVYVVFF"
     gene            complement(19264..19632)
                     /gene="folB"
                     /locus_tag="IU325_09645"
     CDS             complement(19264..19632)
                     /gene="folB"
                     /locus_tag="IU325_09645"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HAP5809291.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            19737..20354
                     /gene="plsY"
                     /locus_tag="IU325_09650"
     CDS             19737..20354
                     /gene="plsY"
                     /locus_tag="IU325_09650"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP5809292.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(20367..21299)
                     /gene="ttdR"
                     /locus_tag="IU325_09655"
                     /gene_synonym="dan"
     CDS             complement(20367..21299)
                     /gene="ttdR"
                     /locus_tag="IU325_09655"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HAP5809293.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            21506..22417
                     /gene="ttdA"
                     /locus_tag="IU325_09660"
     CDS             21506..22417
                     /gene="ttdA"
                     /locus_tag="IU325_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HAP5809294.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIHDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELEVRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLSFENLSHTRSAL"
     gene            22414..23019
                     /gene="ttdB"
                     /locus_tag="IU325_09665"
     CDS             22414..23019
                     /gene="ttdB"
                     /locus_tag="IU325_09665"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HAP5809295.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            23068..24531
                     /locus_tag="IU325_09670"
     CDS             23068..24531
                     /locus_tag="IU325_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HAP5809296.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLNGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPMVLL"
     gene            complement(24574..25587)
                     /gene="tsaD"
                     /locus_tag="IU325_09675"
     CDS             complement(24574..25587)
                     /gene="tsaD"
                     /locus_tag="IU325_09675"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP5809297.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            25825..26040
                     /gene="rpsU"
                     /locus_tag="IU325_09680"
     CDS             25825..26040
                     /gene="rpsU"
                     /locus_tag="IU325_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP5809298.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            26151..27896
                     /gene="dnaG"
                     /locus_tag="IU325_09685"
     CDS             26151..27896
                     /gene="dnaG"
                     /locus_tag="IU325_09685"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP5809299.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            28091..29932
                     /gene="rpoD"
                     /locus_tag="IU325_09690"
     CDS             28091..29932
                     /gene="rpoD"
                     /locus_tag="IU325_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP5809300.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         7460 a         7987 c         7485 g         7069 t
ORIGIN      
        1 ataacgatta ttattaatac attttaattc acaaaacaaa tttattagca ccacctcaac
       61 agtcgatatt catcattatt atatatttgc gttatataag gagatatatc catgtttgat
      121 aaatatagaa aaactctggt agccggagct gtggcgataa ctctgggttt gtcagcaccg
      181 ggggtgatgg cagcaggttt taaacccgca ccacctgccg ggcaactggg tgcggtcatt
      241 gtcgatccct acggcaatgc accactgacc gctttggttg acttagatag ccatgtcatt
      301 tctgatgtca aagttaccgt ccatgggaag ggcgaaaaag gcgtagaaat cagctatccc
      361 gtgggtcagg aatcactaaa aacttatgat ggtgtaccga tttttggtct ttatcagaaa
      421 tttgctaaca aagtgaccgt tgagtggaaa gaaaacggca aggtcatgaa agatgattat
      481 gtggtgcaca cttcggccat cgtcaataat tacatggata accgctctat ctccgattta
      541 caacagacca aagttattaa agtcgcaccg ggttttgaag atcgcctcta tctggttaat
      601 acccacacct ttaccgccca aggttccgat ctccactggc atggtgagaa agataaaaat
      661 gccggtatcc ttgatgcggg tccggcaacc ggcgcactac cttttgatat cgcgcccttt
      721 actttcatcg tcgataccaa aggtgaatac cgctggtggc tggatcaaga cactttctac
      781 gatggtcgtg accgcgatat caacaagcgt ggttatctga tgggtatccg cgaaacgcca
      841 cgcggcacct ttaccgctgt acaaggtcag cactggtacg agttcgacat gatggggcag
      901 gtgctcgaag atcacaaact accgcgcgga tttgctgacg ccactcatga gtccattgag
      961 acgcctaatg gcacagtatt gttgcgcgta ggtaagagca attaccgtcg cgatgacggc
     1021 gtacacgtca ccaccattcg tgaccatatt ctcgaagtcg ataaatctgg tcgcgttgtc
     1081 gatgtatggg atctgaccaa gatcctcgat ccgaaacgcg atgcactgct cggcgcgctg
     1141 gatgcaggtg cagtttgcgt taacgttgac cttgcccatg caggacaaca ggcaaaactg
     1201 gaaccagata caccgttcgg cgacgcgctg ggtgtagggc caggtcgtaa ctgggcgcac
     1261 gttaactcaa tcgcctatga cgcaaaagat gactcaatca tcctctcctc tcgccaccag
     1321 ggtgttgtga aaattggtcg tgataagcaa gtgaaatgga tccttgcgcc ctctaaaggt
     1381 tgggaaaaac cgctggccag caagctgctg aaaccggttg atgctaacgg taagccaatt
     1441 acctgtaacg aaaatggcct gtgcgaaaac tcagacttcg actttaccta cacccagcat
     1501 accgcctgga tttccagcaa aggaacgctc accatttttg ataatggcga tgggcgtcat
     1561 ctggaacaac ctgccttacc aaccatgaaa tactcccgct ttgtggaata taagattgat
     1621 gagaagaaag gcaccgttca gcaagtgtgg gaatacggta aagaacgtgg ctacgatttc
     1681 tatagcccga ttacctccat cattgaatat caagccgacc gtaacaccat gtttggcttc
     1741 ggtggttcta ttcatttgtt cgatgtcggg cagccaaccg tcggtaagtt gaacgaaatc
     1801 gattacaaaa ccaaagaagt gaaagtggaa atcgacgtgc tgtcagataa acccaatcag
     1861 actcactatc gtgcactgtt agtccgtcca caacagatgt tcaaataatt aagaagggaa
     1921 taaaaatgtc gaaattaggg atctcatcac tatttaaaac catactgctt accgcagctt
     1981 tggctgtttc atttaccgct tctgcattta ccgaaggcac ggattacatg gttctggaaa
     2041 aacctattcc gaatgccgac aaaacgctga ttaaggtatt tagctacgcc tgcccgttct
     2101 gttacaagta cgacaaagcg gttaccggtc cggtatcgga aaaagtgaaa gatatcgttg
     2161 cttttactcc attccatctg gaaacaaaag gtgaatacgg caaacaagca agcgaagtct
     2221 ttgcggtctt gatcaataaa gataaagcag caggtatttc attatttgat gctaactctc
     2281 agtttaagaa agccaagttt gcttattacg cggcttatca tgacaaaaaa gaacgctggt
     2341 ctgatggtaa agatccggct gcctttatca aaaccggact ggacgccgca ggtatgagtc
     2401 aggccgattt tgaagccgcg ctgaaagagc ctgcagttca ggaaacgctg gaaaaatgga
     2461 aagcctctta tgaagttgca aaaatccagg gcgttccggc gtatgtcgtc aatggtaaat
     2521 acctgatcta caccaagagt attaaatcta ttgactcaat ggcagacctt atccgcgagt
     2581 tagcaagcaa ataacaggga aacaggctat gggtattaag ggaatgtgga aagaccttcg
     2641 cacttcaccg gtggacacgc tggtaagatg gcaagaacaa cgttttttgt ggttgttaat
     2701 ggccattgcg atgggcgcgt taattatcct ggcgcactct ttcttccaga tctatctcta
     2761 catggcaccc tgtgagcaat gtgtatacat tcgctacgcc atgtttgtga tggttattgg
     2821 aggattggtt gcagcgatca acccaaagaa catcatccta aagctaattg gctgcgtgat
     2881 ggcattttac ggcagcattt tgggactgaa gttttcgctc aaactgaacg atatccacca
     2941 tgcagtacat aatccggatc ccgattcact gtttggcgta cagggttgct ctaccgatcc
     3001 aacttttccc tttaacctgc cgctggcaca gtgggcacca aattggttca agcctaccgg
     3061 ggattgtggc tatgacgcac caatcgttcc tgatggcgtc acactgagca gcacgcaaca
     3121 gtggtttgtg gaaatgtatc agcaatctga aggctggtat ctgctaccac catggcattt
     3181 tatgaatatg gctcaggctt gtatgctggc ttttggtatg tgtctggtgc tgctggtcat
     3241 tatgagtggc gcatgggcgc ttaaaatcat cagaggctaa ctcagcaaca cccgaagcca
     3301 gtcgttctgg cttcgggttc ttttattaac ctatctgcac cgacagcata cttaaactgc
     3361 ccatttctat accatcgaca ggaatggtaa ttggctcctg cccatcccac gcccctaaga
     3421 catacaacaa cggcagatag tgctctggcg ttgggttcga taacgcgcca ccttcatggt
     3481 cgagataatt caccagagga tgttgttcca ctggcccttg ccacgtcaga ttcgctttca
     3541 catactcatt aaacgacgtc gcccacggat acggtgaact atcaccgtgc cacttcactg
     3601 tacgcaggtt atgcaccacg ttgccactgg cgaccaacat tatcccttca tcacgcagcg
     3661 ccgcgagttt gcgccccatt tcgaaatgcc aggcggcagg tttgctactg tcgatactca
     3721 actgcaccat cgggatatcg gcgtcagggt acatcttaat cagcactccc caggaaccgt
     3781 ggtcaaagcc ccaggcttct ttatccagct ccaccgggac cggcgctaac agctcaacca
     3841 gatgctgtgc cagcgcaggc gaacccggag caggataatg cgtgtcgtac agcgcctgcg
     3901 ggaagccacc aaagtcatga atcgtgggcg gcgtctccat cgcggtcact cctgttccac
     3961 gggtaaacca gtgagccgaa accaccacaa tcgcttgcgg gcgtggcaat gtcatcccca
     4021 acttctgcca gctgcgggta tacaaattat cttccagcac gttcatcgga ctaccgtgac
     4081 ctaaaaacaa tgctggcata cgtgttgaag acatgatgat atccttaact aaaggtgtca
     4141 ttttgatatc ctcacaatac gcttgttcgg cggagtaaga acccggataa caatgatgat
     4201 gatcatcagt tattttgacg atctgcctga aggtgaagat ttataaggag ttgtcgatgt
     4261 cagtacctct cattctgacc atactggcgg gggcagccac gtttattggc gcgtttctcg
     4321 gcgttctcgg gcaaaaaccc tcgaaccgct tactggcgtt ttcgctagga tttgcggcgg
     4381 ggatcatgtt gctcatctca ttaatggaaa tgcttcctgc cgcactagca gctgaaggaa
     4441 tgtcgcctgt gttgggttat ggaatgttta tcttcggtct gcttggctat tttggcctgg
     4501 accgcatgtt gccacatgcg catccgcagg atttaatgca aaaatcggtg cagccgttgc
     4561 caaaatcgat caagcgcaca gccattctgc tcactctcgg tatcagtctg cataacttcc
     4621 cggaagggat tgccaccttt gttacagcaa gcagcaacct ggagctgggc tttggcatcg
     4681 cactggccgt tgcgttacac aatattcctg aaggtctggc ggttgcaggc ccggtttacg
     4741 cggcaacggg gtctaaacgc accgcgattc tttgggcggg gatttctgga ctggcagaaa
     4801 ttcttggtgg tgtgctggcg tggttaatcc tcggtagcat gatttccccg gtggtcatgg
     4861 cggcaatcat ggcggcggtt gcaggaatta tggtggcgct ctcggttgat gaattaatgc
     4921 cgctcgccaa agagattgac cctaataata accccagcta tggcgtacta tgtggaatgt
     4981 cagtgatggg attcagttta gtgctgctac aaacggcggg aattggttaa aaacaacatc
     5041 agaagttgtc cagcgcatgg acaacttctg ccagcttcac gaaagcctta ttctaccttt
     5101 tcggctgtct cttctctcgt actgtttaag tatttgttcc gcgcttcaag cagtgcgtct
     5161 ctgtcctggc gaaacgcccg acaataacgt ttcaaatgca ggaaataacc caccacaacc
     5221 agagcaatca atacaatcca gtaccaggcg ataaacatcc ttttaccctt aatatttaac
     5281 aatactaaac atttagcgca taaatttcac atatcctttt tcggatatat tcatatggtc
     5341 gcataataac ggaacaatta tatgattttg atcataatga aaaagaatgc ctgatattaa
     5401 tgaaattttc tcatagaaag agaagcggaa ttaacatctt gcatagcagg gaaaaataaa
     5461 aaccgaagcc tgctacaact tcggtttctt gattaaggca gtaaattaag cagcggtttt
     5521 cagctggctt tacgctcatg tgcctgacga taagccacca ggtcttcaat agtcacgagc
     5581 gccatattgt gtttattggc aaactcaatg cactctggtg cacgcgccat cgtgccatcg
     5641 tcattagtca gctcacacag cacaccagcc ggtttaaagc ctgccagcgt catcagatca
     5701 atagtcgctt cagtatggcc gccacgcgtc agtacaccac cagcctgagc gcgaagtggg
     5761 aaaacgtggc ccgggcgatt cagatcagaa ggttttgcgt catcggcaat cgctgcgcga
     5821 acggtcataa tacggtcagc ggcagaaaca ccggtagtca caccttcagc cgcttcaatg
     5881 gtcacggtaa aaccggtgcc ataggcgctg gtgttatttt ctaccatcat tggcagatca
     5941 agttgtttac ggcgatcttc agtaatgcac aggcaaacaa taccgctacc gtggcgaatg
     6001 gtcagcgcca tctgctcaac ggtcatggtt tctgccggga agatcatatc gccttcgttt
     6061 tcacggtctt catcatcaag caccattaca ccgcgtcctt cacgcagcgc agccagtgca
     6121 ttttcaacac gttcgaaagg cgtaccaaaa gaggaaagta gcgtctgatt catggtaaaa
     6181 aaacctcact aaaattatgg ttaccagaat cagggcagtc ttaggagtgg cggcatatag
     6241 ccaaaataac gtgagcgggt ccatgcccga cagaatcgtt actctctccc atccggactc
     6301 taaccgtcgg ccccggaatt acaccggatc tgctgtcctt tgagttcgca cccaaagcgc
     6361 tcgcgggctt tcaactgagt tgatttaccg ccggtgggga atttcgcccc gccctgagaa
     6421 taagcggatt cactataacg ctaatgatta gcggcagcaa cgcatagctt cacataattc
     6481 tggtttatga cttaccctta tcgcactaca atggcactca acacctatca gtacagggaa
     6541 actacaatga ttgacccgaa aaaaattgag caaatcgctc gccaggttca cgaatcaatg
     6601 cctaaaggaa tcagggagtt cggggaagat gtggagaaaa aaatccgcca aaccctacaa
     6661 gcgcagctga cgcgtctcga tctggtaagc cgcgaagaat tcgacgttca gacgcaagtc
     6721 ctgttacgga ctcgtgaaaa actggcgtta ctggagcagc gcatcagtga actggaagcg
     6781 cgcaataatt cggtagcaga tcttcaatct cctccggcta tcccgcctat tgataaagca
     6841 gaataaagca acacaaaaac gggccagacg gcccgtaata tcagtgttct aattcaagtt
     6901 ctctggtttg catcatctgt tggcaatacc aggcataacg tgcgcaaaac caggttctat
     6961 gttcttcatc catgttacca gtaacggcca gaatatcgac cgggcgacct tctgcgtgca
     7021 ttctggcaaa actacgcgcc aggaaatcga aattatttaa agaattaaga ctatgatcca
     7081 taagcacatc ctccggttat cgcatacctt ttaaggccat atgctttttc tgatttcagt
     7141 attccccaat cagtgtagtg cctttaaaca taacgtgcgt aaatatattg tccccgatca
     7201 cactttttag tgaaaaaata aaaaaccata ttaaaagact ttctcttcca gaacttatct
     7261 aacatgtgtt attattaaca aagataaatg taagcaagga aactatccca aagtaaatat
     7321 tgcgtcacta aatggacatt ggagtgacat atgattttat tcgccgacta taacacccct
     7381 tatcttttcg ctatttcctt tgtactgttg atcggccttt tagaaatttt tgcgcttatc
     7441 tgcggtcaca tgatctccgg tgcacttgat gcacatcttg accattatga ttccataacc
     7501 acaggtcata ttagccaggc acttcattat ctcaatatcg gaagattgcc agctctcgtc
     7561 gttctttgct tactggctgg tttttttggt cttattggca tcctgttgca gcatgcctgc
     7621 ctcatggtct ggcaatcacc actttcgaac ctgttcgtgg ttcctgtcag tttactgttt
     7681 acgattattg cggtgcatta taccggtaaa atagttgcgc cctggattcc acgcgaccat
     7741 agttcagcta ttacagaaga agaatatatt ggtagcatgg cgttaattac cggtcatcag
     7801 gccacttcag gtaatccctg cgaaggaaaa ctcaccgatc aatttggcca aatacattat
     7861 ttactactag agcctgaaga aggaaaaatt ttcactaaag gagacaaagt gttaattatt
     7921 tgccgacttt ctgcaacgcg atatttggcg gaaaacaatc cctggcccca aatattgtaa
     7981 tttattaatc aaaaggaaat ttaataatgg atgatattgt taattctgtg ccctcctgga
     8041 tgtttaccgc gattatttcc gtatgcattc tgtttattat tggaattatt ttcgccaggc
     8101 tctatcgtcg cgcttcggca gagcaagctt ttgttcgtac tggtttaggt gggcaaaaag
     8161 tggtaatgag cggtggcgca atcgtgatgc cgatctttca tgaaataatc cccatcaata
     8221 tgaatactct gaagctggaa gtcagccgct caaccattga tagcctgatt acgaaagatc
     8281 gtatgcgcgt cgatgtcgtt gtcgctttct ttgtgcgggt aaaaccttca gtagaaggta
     8341 ttgccaccgc cgcccagacg ctggggcaac gcaccctgtc gcctgaagac ttacgtatgt
     8401 tggttgaaga taaatttgtc gatgccctcc gtgcaacagc tgcacaaatg accatgcatg
     8461 agttacagga tacccgcgag aacttcgtgc agggcgtaca aaataccgtt gctgaagatc
     8521 tgtcgaaaaa cggcctggaa ctggaaagcg tttcacttac caactttaac cagacgtcga
     8581 aagaacattt caatcctaac aacgcctttg acgccgaagg tttaaccaag ctgacgcagg
     8641 agacggagcg ccgtcgtcgc gaacgtaacg aagttgaaca ggatgtagaa gttgcggtgc
     8701 gtgagaaaaa ccgtgatgca ctttcgcgga agctggagat tgaacaacaa gaagcgttta
     8761 tgacgcttga gcaggagcag caggttaaaa cccgtaccgc tgagcagaat gcgaaaattg
     8821 cggcttttga ggccgagcgt cgtcgggaag cagagcagac gcgaattctg gctgaacgac
     8881 agattcagga aacggaaatc gaccgcgaac aggccgtccg ttccagaaaa gttgaagctg
     8941 aacgtgaagt tcgcattaaa gagatcgaac aacagcaggt caccgaaatc gccaaccaga
     9001 cgaaatcgat cgctattgcc gccaaatcgg aacagcagtc acaagcggaa gcgcgtgcca
     9061 accttgcact tgcagaagcg gtaagcgccc agcaaaacgt agaaacaact cgccagaccg
     9121 cagaagccga tcgtgctaaa caagttgccc taatcgctgc cgcgcaggat gcagaaacca
     9181 aagcggttga actgaccgtg cgggcgaaag cagagaaaga agccgcagag atgcaggcgg
     9241 cggcaatcgt tgagttagcc gaagctacgc gtaaaaaggg tctggcggaa gcagaagcgc
     9301 aacgtgcgct gaacgacgct atcaacgtac tttctgatga acaaaccagc cttaaattca
     9361 aactggcgct attacagtcg ttacctgcag taatagagaa atccgttgag ccgatgaagt
     9421 ctatcgacgg catcaagatt attcaggtcg atggattaaa ccgaggtggt gctgcgggga
     9481 atgcggcatc aggcagcgtt agtggcggaa acctggcaga acaggcatta tcagccgcac
     9541 tctcttaccg cacacaggca ccactgattg actccttgct caatgaaatt ggcgtttcag
     9601 gcggctcact ggcggcattg acttcaccct taacctcaac aactcccgtc gcagaaaacg
     9661 tagaataaat tttcaactat gaacatccct ggcccaaaag gccggggatt ttttatatct
     9721 gcgttccgca aaaaactgca aatgctcagg tcgttgcagc cttttgcgtg accgctcggg
     9781 gaaggcaaaa ctgcctctgg gaaagcattg cgcggggtct gacgctcatc aacaatcggg
     9841 aagtcagcaa agggctgaaa cgggaaagcc cctcccgagg aaggggcctt acagaaggaa
     9901 agggttatga tgaagctcgt catcatactg attgtgttgt tacttgtaag tttcgccact
     9961 tattaacagc caatcagagg ggggagaaat cctccctcac ccttgttcct ttactttagg
    10021 ttgaaaaaaa atcagcgtca agaaggtttc attaacacat atccctggcc caaaaagccg
    10081 gggatttttt atatctgcgt tccgctaaaa ggtgcaaatg atcaggctgt tgcagcgttt
    10141 tgcgtgacca ctcggggaag gcaaaactgc ctcagggaaa gcgttgcgcg gggtccggcg
    10201 ctcatcaaca atcggggagt cagcaagggg ctgaaacggg aaagcccctc ccgaagaagg
    10261 ggccttacag aaggaaaggg ttatgatgaa gttcgtcatc atactggttg tgttgttact
    10321 gttaagtttc ccgacttact aacaactcat cagagggggg agaaatcctc ccttaccctt
    10381 gttcctttac tctaggttga aaaaacaaca gcgtcaatag gcctgccagg tacgaagcga
    10441 gatctgtgaa acgctttccg gttagccttt tttatcctgt tggatcttct tgatgatgtt
    10501 ggtcgtcgag caaccgtctt caaagttgag caccagcact tcgccgccgt tggcccagac
    10561 ttctttactc ccggcaatct cttctggttt atagtcgccg cctttcacca gcaggtctgg
    10621 caagatcccg gcgatcaagc gctgcggcgt gtcttcttca aacgacacca cccagtcgac
    10681 cgcttccagt gcacccagca caatcatacg ctgttcgaga gggttaacgg ggcgggaatc
    10741 ccctttcagc cgtttggtgg aggcatcgct gttcacagcg acaatcaggc ggtcacccag
    10801 tttgcgggca tttgccagat aggagacgtg cccggcatgg agaatgtcga agacgccgtt
    10861 ggtcatcacc actttttcac cgcgtttacg cgctgccgct acagcaagct tcagttcctc
    10921 ttcggtcatc acgccaaagc cggtatctgc acgtccacgt actgcgtttt ccagctcgat
    10981 cggcgaaacc gtggatgtcc ccagtttgcc gaccaccaca ccagccgccg cattggcaaa
    11041 gaagcaggct tcttccagcg aattacccgc tgccagcgtt gccgccagga caccaatcac
    11101 cgtgtcgccc gcaccggtaa cgtcatacac ttcctgcgcc tgggttggca tatgcagcgg
    11161 cgctttaccc ggttgcagca gcgacatacc ctgttcggaa cgggtcacta acagagccga
    11221 gagttcgtaa tcggcaatca gtttcatgcc gcgctcaaca atctcttctt cggtcttaca
    11281 tttaccgaca acagcttcaa attccgagag attcggcgtt aacagcgtag cgccgcggta
    11341 gcgctcaaaa tcggtacctt ttggatcaat cagcaccgga acacccgctt tacgcgccag
    11401 ttggatcatc tgctgtacgc ttgccagcgc acctttggcg tagtcagaaa gcaccagcgc
    11461 gccaatcgaa ctcagcgcct gattaatccg ttcgtgcagc ggttgcggat caacaccttc
    11521 gaaaccttct tcaaaatcca gacggatcag ctgttggttg cgggaaagta cccgtaattt
    11581 ggtgatggtc ggatgcgtcg gtacagaaac gaagtcgcat ttgacgttga cgtcggccag
    11641 agatttactc agcgcgcgcg ctgcatcgtc aatgcccgtc aacccgacca ggcgtgcatt
    11701 agcaccgaga gaagcgatat tcatcgccac gttagccgcg ccgcccggac gttcttcgat
    11761 ggtatttact ttaaccacgg gcaccggcgc ttccggcgag atacggctgg tgggaccgta
    11821 ccagtaacga tccagcatca catcaccaac caccatcact cctgcacgtt caaactctgg
    11881 cagcgttact ttcattcctg tctcctgaga gattcaaaat ttgcgcgcga taataccata
    11941 cttcattctt ccaccagcca cttctgccag cttgcccgca ccagttcacg ctctgcggtg
    12001 aagcaatcct ccgacacatg gcccggcaat tcctgtaatg ccagatgatg aagttcatcg
    12061 cgcagcgtcg tgtaagcacg ggtcagcgcc atcgcttcct gctcttccat aatgtcgttt
    12121 tgcgccagta gttccagaat gcgcacgttg tctgaccagc gcgttaactt cggtttttca
    12181 tgagcgtagc gcaacaccag atattgagta ataaattcga tatcggtaat tccgccttca
    12241 tcggctttga tatcaaagcg atcgcgatgt ttattgccga gatgggcacg cattttctcg
    12301 cgcatttccc gcacttctgt ttgcagggtt ttgccttcgc gcggcagcgt cataatctcc
    12361 cggcgtactg cgtcaaagtg cgcggtgagc tgcggatcgc cgtacactac acgcgcacgc
    12421 accagcgcct gatgttccca cgtccaggcc tcgtttttct gataatcggc aaacgcttct
    12481 gcggatgtca ccagcattcc cgccgccccg gacggacgca accgtgcatc cacctcatac
    12541 aaaatgccgg aagaggttcg cgtactgaac agatgcataa tgcgttgcgc cagacgcaga
    12601 taaaactgtc gcccgtcgat ttcccgctcg ccgtcagtca tcgcatccat cgggcaatca
    12661 tggaggaaga tcaggtcaag atcggaactg tagcctaact cccagccgcc cagcttgccg
    12721 tagccgacca ccgcaaaacc acgcccttcg cgttcgttta ggtgatttgg cttaccgtag
    12781 cgggcaacca tttgaaccca cgcctgctga acgacggcat ctatcatagc ctccgccagc
    12841 caggttaagt gatcgctcac tttcattacc ggtagcgtac cggcgatatc cgctgccgca
    12901 atacgtaaca gttgcgcctg tttgaactga cgcagcgcct caagctgttg ttcttcgtca
    12961 tcttccggca cgcgcagcaa atactggcgc agttcatcac ggtaggcatc catcgccgtc
    13021 ggctggtaga gagtgtttgg atcgagcagt tcgtccagca ataatggata acgcgctagc
    13081 tggctggcaa tcatcggcga tgcggcacac agagaaatca aatgtttaag cgccgcgggg
    13141 aattcactga gcaattctaa ataggtagtg cgggtaacaa ttcccaccag caaggcggta
    13201 atgcgcgaca gcgtgacagc agcgtcttca cgcgcacaga catcacttag cagatgcggc
    13261 atcagatggt cgagcacctg acgcccgcgc ggcccgatgg tacgcttatc cagctctttg
    13321 cgaaaatcgg caatcagcgt cagcacctgt ttgcaatcat cttcgctaag atgcgccagc
    13381 actggcgtag tgtcatcttc ctgcaacgca tcctgccaca gctcacgcca ctgttccgac
    13441 agcgactctt cctgagtttc gctttcgtcg tcgccaatca attcattaaa cacccggcgc
    13501 acattggtca tatgtgcggt cagcgcccca gtcagttgct gccagtcagc aaagtccatc
    13561 gcccacgcca gccgcgcacg gttaagctca tcagaaggaa gcgtctgggt ttgttcgtcg
    13621 ttaatgcttt gcagcaggtt ttccagacgc cgcaggaaca gatacgccac tcgcaattgt
    13681 tcagcatcgt tttcagaaag cagatgcagc gcggcaatga cgctgagcgt tggcagtaaa
    13741 gagcgcgact gcagcgacgg ttcgcgccca ccgcgaatga gctggaacac ctgaacgata
    13801 aattcaattt cgcgaatgcc gcctgcgccg agtttgatat tgtcggtcaa accacgtcga
    13861 cgcacttcac gggcaatcat ccctttcatg ttgcgcagcg actgaatcac gctgaaatcg
    13921 atgtaacgac ggaaaacaaa cgggcgcagc atcgcacgca actcgttagc atagacgcct
    13981 tcgctatcgc ccataatccg cgccttgacc atcgcgtaac gctcccagtc gcgcccctgc
    14041 tcctggtaat aatcctccaa cgcggcaaag ctcagcacca gcgggccact ttcgccaaac
    14101 ggacgcagcc gcatatccac gcgatagacg aagccatcct gcgttggttg atccaacact
    14161 ttaatcagcc gctgccccat gcgggtaaaa aactgcgcgt tatccagttc ccggcgtccg
    14221 ccctgcgtac aaccatgttc cggccaggca aaaatcagat cgatatctga ggagaaattc
    14281 agttccccac cgcccagctt acccatgcct aaaatcagca gcggttgcgc ttcgccctgc
    14341 gcattgcacg gcgttcccca ctcgcggcag caagcgtcat acagccagtc acgcgcggcg
    14401 acaatcagcg tctccgccag atggctgagc tgctgcaata tgctctcttc agtaaccaaa
    14461 gccagcgttt gcgcccaggc gatgcgcacc ataatgcgcc gccggaatag ccgcagctcg
    14521 cgcattaacc cggcttcgtc actcacattg ctgagcgcct cctgcaacca tgccgcgtaa
    14581 tgctgccatt cgtcggcctg cggcgattgg ctttccagtt ccgtcagcca ctctggatgc
    14641 gcaatcacgc tgtcctgcac aaaatcactg aaagtaagta ctgacttcgc ctgtgcgcta
    14701 agtgatttct cggctaaagg ctctggcagc cgctcaacaa cggtctgcca gtactgctgt
    14761 aacggtgaag agagcggctt cataagcgat tttatccttg cctgattaac gttttccgct
    14821 gtgcaaccag aacggttcct gattgtttgc ctcattacgg aaatgttcaa tttcaatgcg
    14881 ttgcccggtc gcaatagcgt gacgcagccc ctgccagttc tccagccagg cttgcgcgac
    14941 gacaggatca tagtaacccg ccagcaacag tattgagtta atatcacgcg tcagacgtgg
    15001 caactggtca cggtagcgat cgcccaacgg ctggcagaaa acgcttttca gttcagcggc
    15061 atggcgggaa agatggatat cggcaaagcg tttgaaggaa tcgctcattt tactctgcgc
    15121 tttggcatct aaaaacggct gccaggcttt gcttaccagc cattcggtca acgccagctt
    15181 cgccattgcc gttttggtag agtagaccgc cgtcacggca gaaaccgcag aagcgatggt
    15241 cgcttcgcat tgagtcagca aatcacgtaa gtgagtgctc gctttgcgcg gcacaatacc
    15301 accgaacagc atcagtgtat gacggaccag accaatggct gccagcacct gttctttcgc
    15361 cgcatcgttg ccgcgtaccc acagttcttc atgatactgc cattgcgcta acgccagctc
    15421 gagcgccgct tccagcccct gttccacatc ggcttttgcc gcaacatgca aaatggtggt
    15481 cggtttgatt tcacgcgccg gattgccctg cgccagatga tagccacgcg ccgctttgct
    15541 caggctgccc tggcgtaatc cggtttgcga taccagttgg ttcgccagtt tcagcaccgc
    15601 gcgcgtgtcg ccgctaagca gttccagttc cagctcacag ataggttcag caaattcccc
    15661 cgctttcacc tccccctgat cgagagcgat ttcaattcgg ctgtcatcga ccgccaccag
    15721 ccatttttcg cgataaaaat cggtgctgaa cagcggttgt acgcgggaag cgagatcggc
    15781 gggcaattcg ccgttcggcc agacttccgt cggtaactgc gccaggtcga gcgtcggttc
    15841 gctcaacgcc acgttatatt ccgggcgctg gtgtaagccg cctgtcactc ttcctgcaac
    15901 tttcatggtc atctcatagc gaccgttttc gccacgaata cgtaagccca tatcgtgccc
    15961 acgcagccag ttatccggcg tttcgtagta aatattcagc aactgcacgg ggtcatggtg
    16021 ctcgccgccc agcgtattga gatggtcacg caacgcctca acggcactgt gattaacaat
    16081 aaactttaat tcgatttcct gagccatggc ctttacttat cggttatgtc acataagtga
    16141 cgatgaacgg cgaacttaat gcgatctttt ttgtcagtag atagtatttt gcgccaaatt
    16201 gccatgcaac gggcaatttg acgggcgtaa aagtttgaag cagtggcaag acctgacaca
    16261 gaggattccg attgatgtcg aatgctttgc gtagcgacac tgatgccact actatcgttc
    16321 cactttccat gacaataacg acagcctgat gccaaaatta cgcctgatcg gattaacttt
    16381 actcgcactt agcgccactg ccgtctcaca cgctgaagaa acgcgctatg tttctgatga
    16441 actgaatacc tgggtccgta gcggtccggg agatcattat cgcctcgtgg gcacggttaa
    16501 cgccggcgag gaagtgacct tattacaaac tgacgccaac accaattatg cccaggtgaa
    16561 agacagctct ggccgtaccg cctggatccc gttgaaacaa cttagcactg agccaagtct
    16621 gcgctcccgt gtgccagatc tggaaaatca ggtcaaaacc ctgaccgata aactcaccaa
    16681 tatcgataac acctggaatc agcgcaccgc agaaatgcag caaaaagtgg cgcagagcga
    16741 cagcgtgatc aacgggttaa aagaagaaaa tcagaaactg aaaaacgagc tgattgtcgc
    16801 gcagaaaaag gtcgatgccg ccagcgtaca gctggatgac aaacagcgca ccatcatcat
    16861 gcaatggttt atgtatggcg gcggcgtgct ggggcttggc ttgctgctcg gtctggtact
    16921 gccgcacctg atcccaagcc gcaaacgtaa agatcgctgg atgaactaaa tcgccttctt
    16981 cgttgcactg tcttattatt aggaattgtg tatttgacga agggatgatg gcgtgaagat
    17041 ttatctggtc ggtggtgctg ttcgggatgc attgttaggg ctaccggtca aagacagaga
    17101 ttgggtggtg gtcggcagta cgccacagga gatgctcgac gcgggctacc agcaggtagg
    17161 ccgcgatttt cctgtttttc tgcatccgca aacgcatgaa gagtatgcgc tggcacgtac
    17221 tgaacggaaa tccggttccg gttacaccgg ttttacctgc tatgccgcac cggatgtcac
    17281 gctggaagat gatcttaagc gtcgcgatct gaccattaat gcgctggccc aggacgataa
    17341 cggcgagatt atcgacccgt acaacggtct gggcgatctg caaaatcgtc tgttgcgcca
    17401 tgtttccccc gcttttggcg aagatccgtt acgcgtactg cgcgtggcgc gttttgctgc
    17461 gcgttatgcc cacctcggtt ttcgtattgc cgatgaaact ctggcactga tgcgcgagat
    17521 gacccatgcg ggtgaactgg aacacctgac gcctgaacgg gtatggaaag agacggaaag
    17581 cgccctcacc acccgcaatc cccaagtgtt cttccaggta ctgcgcgatt gcggcgcact
    17641 gcgcgtttta ttcccggaaa ttgacgccct gttcggcgtt ccggcccctg ccaaatggca
    17701 tccggaaatc gacacgggta ttcatacctt aatgacgctc tcaatggcgg cgatgctgag
    17761 tccgcaggtc gatgtccgtt tcgcaacttt atgccacgat ctcggtaaag gactgacgcc
    17821 gccagaactc tggccgcgtc atcatggtca tggcccggcg ggtgttaagt tagtggaaca
    17881 actatgccag cgtctgcgcg tgccaaatga aattcgcgat ttagccagac tggtggctga
    17941 gtttcacgat ctcatccaca ccttcccaat gctgaaccca aaaaccatcg tcaaattgtt
    18001 tgattccatt gacgcctggc gtaaaccgca gcgtgtcgag caactggcgc tgaccagcga
    18061 ggctgacgtg cgcggcagaa ccggttttga atcggcggac tacccgcaag gccgctggtt
    18121 gcgcgaagcc tgggaagtgg cacagtcagt accaacaaaa gccgtcgttg aagcgggatt
    18181 taaaggtgtg gagattcgcg aggagctgac ccgacggcgg attgcggcgg tagccagttg
    18241 gaaggaacaa cgttgcccaa agcctgatta agcacttaaa tttcaatata ttgcaaacac
    18301 cgtccaggtc ggataagacg cacatgcgtc atatccgaca ctatccctca atcaaaagaa
    18361 cacgacgtac accgcagccg ccaaaataaa gcgataaatg gcgaatggga taaacgaaat
    18421 ccgcttaatc aattgcagga aggttttaat cgctatcagc gccactacga aggcagtgat
    18481 aaacccaacg gcaaacatcg ggatatcgcc ggttgtcagg aagccccagc ttttgtagag
    18541 atcaagcgcc gttgcgccca tcatcatcgg caccgccagc aggaacgaga actcggaagc
    18601 agcgtaacgg ctcacgccca tcagcatccc acctgaaatg gtcgccccgg agcgagagaa
    18661 acccggccac agcgccagac actggaagca accaatcata aacgcctgac gataggtcat
    18721 atcatcgaga cccggcgcac gcggttcttt tggtttcagg cattcggcgg caatcagcaa
    18781 cagaccgcca acgaccagcg catacatcac atttatcggg ttaaacaacg acttaatcgt
    18841 gtcgtggaac aacagcccca gtaccaccgc cggaatcatc cccagcaaaa tgtggatcag
    18901 cgttaaacgg cctttgcttt ctccttcgtg ctgcaacggg cgaccaaagt gaataccaat
    18961 aaggccaaac agacgccgcc agaacatcac tactaccgcc agaattgatc ctaactggat
    19021 cacgacttca aaggttttcg ccgtgtcgcc ctcaaacccc aacaagtgac cgacaataat
    19081 catatggccc gtgctggata ccggcagaaa ttctgtcaat ccttcgacca cacccaatat
    19141 tgccgctatc agcagcgagt gcatatcgct catcaataaa cccctaaatt ataaaaatgt
    19201 aacgcttgtc cgaactactg cagtatgacc aggttataac cgtttggttt aacagctgta
    19261 aaattaatta ttttctttca gattattgcc acgctcaatg atgacgccaa cattcgccgc
    19321 ccgcgccact gcgcctggct tgctgagttt gatacgcacc cacggcgagt tgaagcgtgc
    19381 taacagcagc tccgccacct cttccgccac acgttccacc agcgcaaaac gcgccccctc
    19441 gacgtggctg actaccgttt ctgcaatgtc agcgtaactg aggcaatccg ccacatcatc
    19501 acttttcgcc gctttacggt tatcccacgc catttcgata tcgaacacta acttctgttc
    19561 gatggtctgt tcccagtcgt aaacaccaat agtggtgatt accgaaagtt gctctataaa
    19621 tacaatatcc atcacgtcct gcctgctttt tggctaaccg gataccactt ccggcgaaat
    19681 gtgcgtatta tccacagatt catcgttgaa cacgaatttt caaaacggaa cagcttatga
    19741 gtgcaatcgc gcctggaatg atcctcatcg cgtacctctg cggctccatt tccagtgcca
    19801 ttctggtttg ccgcttgtgt gggctgcccg atccgcgaac cagcggctcc ggcaatcctg
    19861 gcgcaaccaa tgtgttacgt atcggtggca agggagcagc cgtagcagta ctgattttcg
    19921 acgttctgaa aggaatgttg cctgtctggg gggcatatga attaggtgtc agccccttct
    19981 ggctaggctt aattgccatc gccgcctgtc ttggacacat ctggcccgtt ttcttcggtt
    20041 ttaaaggagg aaaaggcgtt gctaccgctt ttggtgccat cgcaccaatt ggctgggatc
    20101 ttactggagt aatggcggga acctggttac tgaccgtgct attgagcgga tattcgtcgc
    20161 tgggagcgat tgtcagtgca ctgattgctc cgttttatgt ctggtggttt aagccacaat
    20221 tcaccttccc ggtttcgatg ctctcttgcc tgatcctgct gcgtcatcat gacaacatcc
    20281 agcgtctgtg gcgtcgtcaa gagacaaaaa tctggacgaa attcaaaaga aagcgcgaaa
    20341 aggatcctga gtgatttttc gcgagtctac ataacctgat agccttcgtc gggcttgccc
    20401 agccgttgct ggcaccatgc cgccagaaat tccacgcaga cacgtaattt catgctgcga
    20461 tagagcggct cccggtaaac agcccagata ttggcgcttt gtgcatactc cggcaatact
    20521 cgcaccagtt tgccactctc cagaaacggc aacacatccc actcggaacg caacataatc
    20581 cctttgccct ccagcgccca ttgcaacact atctcgccgc tattggagga aagatgcccg
    20641 cttaccttca ccgatttttt ctcctgaccg ttccccaact cccatattcc gtgggtcata
    20701 tcgcgctctt tggtcaccag acaatcatga cgacttaatt cctgtaagga ttgtggctgc
    20761 ggatattttt gcagatactc aggtgctgcg cataatattc ttttattttt cgttaacaga
    20821 tgcgcaatat aataatcagg aatttcgtca ttaatacgaa tatccagatc aatattatcc
    20881 tgcactaaat caatttgccg atcgaacaat tcaaaatgca cctgtaactc aggataattg
    20941 cgcatcagtt cggtaatggc tggcgcaata tggctgcgcc caaaaccaaa actgcaacca
    21001 atacgaatca tcccctccgg gcgcgttttg atttgcgtga cgtcatcgac cagtcgctga
    21061 tattgggtga ggatttccag cgcatgctcg taacaacgtt ggccactttc agtcagcgcc
    21121 acgccgcgag cggagcggtt cagcagcgtt gttgccagcg tattttcaag gatttggatc
    21181 cgcttggtga caaatgcagg cgtctgccca agcgtcgccg ctgcggcgct aaaactcccg
    21241 ctgtggacga tttctaccag tacctgcaaa tctttggcta aaggccagct attcagcatc
    21301 gttaattatc cgcagttgtg ataagcgcag tgtatttcgc aaaacattgc cattccctgc
    21361 tctggcgcaa tattcattct tattttcgcg ttgattcacg aactgttaat tacatattca
    21421 cgctggcaaa aaatataaaa ccacattttt agtggtagtt tgttgtggtg aatttttcca
    21481 gacaaataaa gaaactggag ttgccatgat gagcgaaagt aataagcaac aggcagtgaa
    21541 taagttgacg gagattgtcg ctaactttac cgcaatgatt tctacccgaa tgcctgatga
    21601 cgtggtggat aaactaaaac agctaaagga tgccgaaacg tcgtcgatgg ggaaaattat
    21661 ctaccatacg atgttcgaca acatgcaaaa agcgattgac ctgaatcgtc ctgcctgtca
    21721 ggacaccggc gaaatcatgt tttttgttaa ggtcggttcc cgtttcccac tgcttggcga
    21781 gctgcaaagc atactcaaac aagccgtgga agaggcaacc gtcaaagcgc cactgcgtca
    21841 caatgcggta gaaatttttg acgaagtaaa taccggcaaa aataccggta gcggcgtacc
    21901 gtgggtcacc tgggacatca tccacgacaa tgacgatgcg gaaatcgaag tttacatggc
    21961 aggcggcggc tgcacgctac caggccgctc gaaagtgtta atgccgtcag aaggctacga
    22021 aggcgtagtg aaattcgtct tcgaaaatat ctccaccctc gccgtaaacg cctgtccacc
    22081 ggtactggtg ggcgtgggca tcgccacctc ggtggaaacc gccgccgtgc tctcgcgaaa
    22141 agccattttg cgcccgattg gcagccgcca tccaaatcca aaagcggcag agctggaagt
    22201 acgcctggaa gaaggactca accgtctggg gattggtcca caagggctga ctggcaacag
    22261 ttcagtgatg ggcgtgcata tcgaatctgc cgcccgccat ccgtcaacca tcggcgttgc
    22321 tgtttctaca ggctgctggg cgcatcgtcg cggcacactg ctggttcatg ccgatctctc
    22381 ctttgaaaat ctgtctcaca cccggagcgc gttatgaaaa agatcctgac aaccccgatc
    22441 aaagctgaag atctgcaaga tattcgcgtc ggcgatgtga tctacctgac cggtacgctg
    22501 gtgacctgcc gtgacgtttg tcaccgccgc ttgatcgaac tgaaacgtcc gatcccttac
    22561 gatctcaacg gcaaagcgat tttccacgct ggccccatcg tgcgcaaaaa cggcgacaaa
    22621 tgggagatgg tttccgtcgg tccgacaacc agtatgcgta tggaaagttt tgaacgtgaa
    22681 tttatcgaac agaccggcgt gaaactggtg gtcggcaaag gcggaatggg gccgctgacc
    22741 gaagaaggct gccagaaatt caaggcgctg catgtgattt tcccggcagg ctgcgcagta
    22801 ctggcggcaa cccaggtgga agagattgaa gaggtgcact ggacagaact cgggatgccg
    22861 gagtcgctgt gggtctgccg ggtcaaagag ttcggtccgc tgattgtctc tattgatacc
    22921 cacggcaaca acctgatagc cgaaaacaaa aagctgttcg ccgaacgccg cgatcccatc
    22981 gtggaagaga tttgcgagca cgtccactac atcaaataac cctcccggag aggctcaccc
    23041 cctctccttt ttcgcaggca taacacgatg aaaccttcca ctgaatggtg gcgatacctt
    23101 gcgccgctgg cggtcatcgc cattattgct ctaattccgg ttcccgcagg gctggagagt
    23161 catacctggc tctactttgc cgtttttact ggcgtgatcg ttggactgat cctcgaaccc
    23221 gtgccgggtg ccgtggtggc gatggtgggt atctccatta tcgccattct ctcgccgtgg
    23281 ctgctgttta gcccggagca gctcgcccag ccaggtttta agttcaccgc caaatccctc
    23341 tcctgggcgg tttctggttt ttctaattcg gttatctggc tgattttcgc cgcctttatg
    23401 tttggcacag gctatgaaaa aaccgggctg gggcgacgta tcgcgctgat tctggtgaaa
    23461 aagatggggc atcgtacgct gtttctcggc tatgcggtga tgttctccga gctgatcctg
    23521 gcacctgtaa caccgtccaa ctcggcgcgc ggtgcgggga ttatctatcc catcatccgt
    23581 aacctgccac cgctctatca atcacaacca aacgacagca gttcgcgcag cattggctcg
    23641 tacatcatgt ggatggggat tgttgccgac tgcgtgacca gcgccatttt cctgacggcg
    23701 atggcaccta acttgctgtt aattggcctg atgaaaagcg catctcacgc cacactgagt
    23761 tggggcgact ggttcctcgg gatgttgccg ctcagtattt tactggttct gctggttccc
    23821 tggctggctt acgtgctgta cccgccggta ctgaagtctg gtgatcaggt gccgcgctgg
    23881 gcagagacgg aactgcaggc aatgggcccg ctctgttctc gtgaaaaacg gatgctgggg
    23941 ctgatggtag gcgcactggt gctgtggatt ttcggcggtg attatatcga tgccgcgatg
    24001 gtcggttaca gcgtggtggc actgatgctg cttctgcgca ttatcagttg ggacgacatt
    24061 gtcagtaata aagcggcgtg gaacgttttc ttctggctgg cctcgcttat caccctcgct
    24121 accggactca acaacaccgg ttttattagc tggtttggca aactgttagc aggcagctta
    24181 aacggttatt cgccaacgat ggtgatggtg gcgttgattg tggtgtttta tctgctgcgc
    24241 tacttctttg ccagcgccac ggcgtatacc tccgccctcg caccgatgat gattgccgct
    24301 gcgctggcga tgccggaaat cccgcttccc gtgttctgcc tgatggttgg cgcggcaatt
    24361 ggtctgggga gcattcttac gccatacgcc accggaccca gtccgattta ctacggtagt
    24421 ggttatctgc caacggtgga ttactggcga ctgggggcaa tttttgggct gatattcctc
    24481 gtgttgctgg tgattaccgg cttactgtgg atgcccatgg tgttgcttta agtgaggacg
    24541 gtgccggaag tgactctccg gcacccgaga gttttacgca gccggtaact cagccagcgg
    24601 ccagcgcgga cgcacgctaa cgccgagatc cgccgtcgcg cctgctttaa accgcaccat
    24661 tccggcatag gcgatcatcg cgccgttatc agtacaaaat tccggacgcg cgtagaacac
    24721 ttcgccacgg cgttttttca tcatttcagc cagcttcgcc cgtaacgtac ggttagcact
    24781 cacgccgccc gccatgacca gtcgcttaaa gcccgtctga tccagcgccc gcttgcactt
    24841 aatcatcagc gtatcgacca ccgcatcttc aaaggcgcgg gcgatatcag cacgcgtctg
    24901 gtcgtcggtg ccgttgtcac gaatggtatt tgccgcgaag gttttcaggc cggagaagct
    24961 gaaatccagc cccggacggt cggtcatcgg gcgcgggaag acaaagcgcc cggctgtacc
    25021 ctgagccgcc attttcgaca gtaatggccc gccaggataa tccagcccca gcagcttcgc
    25081 ggttttatca aacgcttcac cagccgcatc atcgatagac tcgccgagca gctcgtactg
    25141 accaatgcca gtcacgctga ttaactgcgt atgaccgccg gaaaccagca gcgcgacaaa
    25201 cggaaattcc ggtgggttat cttccagcat cggcgctaac agatgccctt ccatatggtg
    25261 taccgggatc gccggaacgt cccaggcaaa cgccagagaa cgccccacgg tcgcgccaac
    25321 cagcagcgcg ccgactaatc cagggcctgc ggtataggcc acagcatcaa tgtcttttgc
    25381 cgttaaacca gactccttta gcgccgcctg gatcaacggt acggttttac gcacatgatc
    25441 gcgggaggcc agttcaggca cgacgccgcc gtagtcagcg tgcaatttca cctgactata
    25501 caattggttg gctaacaaac ctttttcatc gtcgtaaatg gcgatgccgg tttcatcgca
    25561 ggaagtttca atacccagta cacgcatgac ttgttttacc tcgctttatt accgcgcagt
    25621 gtaggaccaa tgcgggttga tgtaaaactt tgttcgcccc tggagaaagc ctcgtgtata
    25681 ctcctcaccc ttataaaagt ccctttcaaa aaaggccgcg gtgctttaca aagcagcagc
    25741 aattgcagta aaattccgca ccattttgaa ataagctggc gttgatgcca gcggcaaacc
    25801 gaattaatca aaggtgagag gcacatgccg gtaattaaag tacgtgaaaa cgagccgttc
    25861 gacgtagctc tgcgtcgctt caagcgttcc tgcgaaaaag caggtgttct ggcggaagtt
    25921 cgtcgtcgtg agttctatga aaaaccgact accgaacgta agcgcgctaa agcttctgca
    25981 gtgaaacgtc acgcgaagaa actggctcgc gaaaacgcac gccgcactcg tctgtactaa
    26041 ttccccgaga gcgttgctct ccgatcagac cgagttgtag ttgtaaggcc gtgcttccga
    26101 aaggaatgcg cggcttattt tcgtttatga attgctaaaa atcggggcct atggctggac
    26161 gaatcccacg cgtattcatt aatgatctgc tggcacgcac tgacatcgtc gatctgatcg
    26221 atgcccgtgt gaagctgaaa aagcagggca agaatttcca cgcgtgttgt ccattccaca
    26281 acgagaaaac cccatcattc accgttaacg gtgagaaaca gttttaccac tgctttggat
    26341 gtggcgcgca cggcaacgcg atcgacttct tgatgaacta cgacaagctt gagttcgtcg
    26401 aaacggtcga agagctggca gcaatgcaca atcttgaagt gccatttgaa gcaggcagcg
    26461 gccccagcca gatcgagcgc catcaacggc aaacgcttta tcagttgatg gacggtctga
    26521 atacgtttta ccaacaatct ttacagcaac ctgttgccac gtctgcgcgc cagtatctgg
    26581 aaaaacgcgg attaagccac gaggttatcg cccgctttgc gattggtttt gctccccccg
    26641 gctgggacaa cgtcctgaag cggtttggcg gcaatccaga aaatcgccag tcattgattg
    26701 atgcgggcat gttggtcact aacgatcagg gtcgcagtta cgatcgtttc cgcgagcggg
    26761 tgatgttccc cattcgtgat aaacgcggtc gggtgattgg ttttggcgga cgcgtgctgg
    26821 gcaacgatac ccccaaatac ctgaactcgc cggaaacgga cattttccat aaaggccgcc
    26881 agctttacgg tctttatgaa gcgcagcagg ataacgctga acccaatcgt ctgcttgtgg
    26941 tcgaaggcta tatggacgtg gtggcgctgg cgcaatacgg cattaattac gccgttgcgt
    27001 cgttaggtac gtcaaccacc gccgatcaca tacaactgtt gttccgcgcg accaacaatg
    27061 tcatttgctg ttatgacggc gaccgtgcag gccgcgatgc cgcctggcga gcgctggaaa
    27121 cggcgctgcc ttacatgaca gacggtcgtc agctacgctt tatgtttttg cctgatggcg
    27181 aagaccctga cacgctggta cgaaaagaag gtaaagaagc gtttgaagcg cggatggagc
    27241 aggcgatgcc actctccgca tttctgttta acagtctgat gccgcaagtt gatctgagta
    27301 cccctgacgg gcgcgcacgt ttgagtacgc tggcactgcc actgatatcg caagtgccgg
    27361 gcgaaacgct gcgaatatat cttcgtcagg aattaggcaa caaattaggc atacttgatg
    27421 acagccagct tgaacgatta atgccaaaag cggcagagag cggcgtttct cgccctgttc
    27481 cgcagctaaa acgcacgacc atgcgtatac ttatagggtt gctggtgcaa aatccagaat
    27541 tagcgacgtt ggtcccgccg cttgagaatc tggatgaaaa taagctccct ggacttggct
    27601 tattcagaga actggtcaac acttgtctct ctcagccagg tctgaccacc gggcaacttt
    27661 tagagcacta tcgtggtaca aataatgctg ccacccttga aaaactgtcg atgtgggacg
    27721 atatagcaga taagaatatt gctgagcaaa ccttcaccga ctcactcaac catatgtttg
    27781 attcgctgct tgaactgcgc caggaagagt taatcgctcg tgagcgcacg catggtttaa
    27841 gcaacgaaga acgcctggag ctctggacat taaaccagga gctggcgaaa aagtgattta
    27901 acggcttaag tgccgaagag cgatcgggaa gcccccgaca gccgcactga gaggcagcgg
    27961 caaatatata agtacgccct cgtaattatc gttggcggta aacaaccgtt ggatttcagc
    28021 gttaacggct gaaggacatc gggtcaatcg cccaacacca acctcatgaa ataagtgtgg
    28081 ataccgtctt atggagcaaa acccgcagtc acagctgaaa cttcttgtca cccgtggtaa
    28141 ggagcaaggc tatctgacct atgccgaggt caatgaccat ctgccggaag atatcgtcga
    28201 ttcagatcag atcgaagaca tcatccaaat gatcaacgac atgggcattc aggtgatgga
    28261 agaagcaccg gatgccgatg atctgatgct ggctgaaaac accgcggacg aagatgctgc
    28321 cgaagccgcc gcgcaggtgc tttccagcgt ggaatctgaa atcgggcgca cgactgaccc
    28381 ggtacgcatg tacatgcgtg aaatgggcac cgttgaactg ttgacccgcg aaggcgaaat
    28441 tgacatcgct aagcgtattg aagacgggat caaccaggtt caatgctccg ttgctgaata
    28501 tccggaagcg atcacctatc tgctggaaca gtacgatcgt gttgaagcag aagaagcgcg
    28561 tctgtccgat ctgatcaccg gctttgttga cccgaacgca gaagaagatc tggcacctac
    28621 cgccactcac gtcggttctg agctttccca ggaagatctg gacgatgacg aagatgaaga
    28681 cgaagaagat ggcgatgacg acagcgccga tgatgacaac agcatcgacc cggaactggc
    28741 tcgtgaaaaa tttgcggaac tgcgcgctca gtacgttgta acgcgtgaca ccatcaaagc
    28801 gaaaggtcgc agtcacgctg ccgctcagga agagatcctg aaactgtctg aagtattcaa
    28861 acagttccgc ctggtgccga agcagtttga ctacctggtc aacagcatgc gcgtcatgat
    28921 ggaccgcgtt cgtacgcaag aacgtctgat catgaagctc tgtgttgagc agtgcaaaat
    28981 gccgaagaaa aacttcatta ccctgtttac cggcaacgaa accagcgata cctggttcaa
    29041 cgcggcaatt gcgatgaaca agccgtggtc ggaaaaactg cacgatgtct ctgaagaagt
    29101 gcatcgcgcc ctgcagaaac tgcagcagat tgaagaagaa accggcctga ccatcgagca
    29161 ggttaaagat atcaaccgtc gtatgtccat cggtgaagcg aaagcccgcc gtgcgaagaa
    29221 agagatggtt gaagcgaact tacgtctggt tatttctatc gccaagaaat acaccaaccg
    29281 tggcttgcag ttccttgacc tgattcagga aggtaacatc ggcctgatga aagcggttga
    29341 taaattcgaa taccgccgtg gttacaagtt ctccacctac gcaacctggt ggatccgtca
    29401 ggcgatcacc cgttctatcg cggatcaggc gcgcaccatc cgtattccgg tgcatatgat
    29461 tgagactatc aacaaactca accgtatttc tcgccagatg ctgcaagaga tgggccgtga
    29521 accgacgccg gaagaactgg ctgaacgtat gctgatgccg gaagacaaga tccgcaaagt
    29581 gctgaagatc gccaaagagc caatctccat ggaaacgccg atcggtgatg atgaagattc
    29641 gcatctgggg gatttcatcg aggataccac cctcgagctg ccgctggatt ctgcgaccac
    29701 cgaaagcctg cgtgcggcaa cgcacgacgt gctggctggc ctgaccgcgc gtgaagcgaa
    29761 agttctgcgt atgcgcttcg gtatcgatat gaacaccgac cacacgctgg aagaagtggg
    29821 taaacagttc gacgttaccc gcgaacgtat ccgtcagatc gaagcgaagg cgctgcgcaa
    29881 actgcgtcac ccgagccgtt ctgaagtgct gcgtagcttc ctggacgatt aatcggtagg
    29941 ccggatcagg tgttacgccg tacccggcac tagtccctcc gcacaaacgc caccttttcg
    30001 g
//
LOCUS       DACBVI010000045        29092 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.045, whole genome shotgun sequence.
ACCESSION   DACBVI010000045 DACBVI010000000
VERSION     DACBVI010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.045"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            345..1913
                     /gene="cydA"
                     /locus_tag="IU325_09695"
     CDS             345..1913
                     /gene="cydA"
                     /locus_tag="IU325_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HAP5809301.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            1929..3068
                     /locus_tag="IU325_09700"
     CDS             1929..3068
                     /locus_tag="IU325_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAP5809302.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            3083..3196
                     /gene="cydX"
                     /locus_tag="IU325_09705"
     CDS             3083..3196
                     /gene="cydX"
                     /locus_tag="IU325_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HAP5809303.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3196..3489
                     /gene="ybgE"
                     /locus_tag="IU325_09710"
     CDS             3196..3489
                     /gene="ybgE"
                     /locus_tag="IU325_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HAP5809304.1"
                     /translation="MSKIIATLYVVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3639..4043
                     /gene="ybgC"
                     /locus_tag="IU325_09715"
     CDS             3639..4043
                     /gene="ybgC"
                     /locus_tag="IU325_09715"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HAP5809305.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            4040..4732
                     /gene="tolQ"
                     /locus_tag="IU325_09720"
     CDS             4040..4732
                     /gene="tolQ"
                     /locus_tag="IU325_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HAP5809306.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4736..5164
                     /gene="tolR"
                     /locus_tag="IU325_09725"
     CDS             4736..5164
                     /gene="tolR"
                     /locus_tag="IU325_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HAP5809307.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5229..6527
                     /gene="tolA"
                     /locus_tag="IU325_09730"
     CDS             5229..6527
                     /gene="tolA"
                     /locus_tag="IU325_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HAP5809308.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAVADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAA
                     AEKAAADKKAAAAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAG
                     SGNTKNNGASGADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPE
                     GGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            6660..7952
                     /gene="tolB"
                     /locus_tag="IU325_09735"
     CDS             6660..7952
                     /gene="tolB"
                     /locus_tag="IU325_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HAP5809309.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            7987..8508
                     /gene="pal"
                     /locus_tag="IU325_09740"
     CDS             7987..8508
                     /gene="pal"
                     /locus_tag="IU325_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HAP5809310.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8518..9309
                     /gene="cpoB"
                     /locus_tag="IU325_09745"
     CDS             8518..9309
                     /gene="cpoB"
                     /locus_tag="IU325_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HAP5809311.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9474..9549
                     /locus_tag="IU325_09750"
     tRNA            9474..9549
                     /locus_tag="IU325_09750"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9507..9509,aa:Lys,seq:ttt)
     gene            9585..9660
                     /locus_tag="IU325_09755"
     tRNA            9585..9660
                     /locus_tag="IU325_09755"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9618..9620,aa:Val,seq:tac)
     gene            9663..9738
                     /locus_tag="IU325_09760"
     tRNA            9663..9738
                     /locus_tag="IU325_09760"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9696..9698,aa:Lys,seq:ttt)
     gene            9790..9865
                     /locus_tag="IU325_09765"
     tRNA            9790..9865
                     /locus_tag="IU325_09765"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9823..9825,aa:Val,seq:tac)
     gene            9869..9944
                     /locus_tag="IU325_09770"
     tRNA            9869..9944
                     /locus_tag="IU325_09770"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9902..9904,aa:Lys,seq:ttt)
     gene            10091..10166
                     /locus_tag="IU325_09775"
     tRNA            10091..10166
                     /locus_tag="IU325_09775"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10124..10126,aa:Lys,seq:ttt)
     gene            10200..10275
                     /locus_tag="IU325_09780"
     tRNA            10200..10275
                     /locus_tag="IU325_09780"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10233..10235,aa:Lys,seq:ttt)
     gene            10553..11596
                     /gene="nadA"
                     /locus_tag="IU325_09785"
     CDS             10553..11596
                     /gene="nadA"
                     /locus_tag="IU325_09785"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HAP5809312.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            11634..12353
                     /gene="pnuC"
                     /locus_tag="IU325_09790"
     CDS             11634..12353
                     /gene="pnuC"
                     /locus_tag="IU325_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HAP5809313.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASMEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(12350..13291)
                     /gene="zitB"
                     /locus_tag="IU325_09795"
     CDS             complement(12350..13291)
                     /gene="zitB"
                     /locus_tag="IU325_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HAP5809314.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREISEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(13405..13785)
                     /locus_tag="IU325_09800"
     CDS             complement(13405..13785)
                     /locus_tag="IU325_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809315.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQSNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14101..15153
                     /gene="aroG"
                     /locus_tag="IU325_09805"
     CDS             14101..15153
                     /gene="aroG"
                     /locus_tag="IU325_09805"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HAP5809316.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYAARLLTLREELQDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGTPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(15319..16071)
                     /gene="gpmA"
                     /locus_tag="IU325_09810"
     CDS             complement(15319..16071)
                     /gene="gpmA"
                     /locus_tag="IU325_09810"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP5809317.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(16274..17314)
                     /gene="galM"
                     /locus_tag="IU325_09815"
     CDS             complement(16274..17314)
                     /gene="galM"
                     /locus_tag="IU325_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HAP5809318.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGSTLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAYVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAQ"
     gene            complement(17308..18456)
                     /gene="galK"
                     /locus_tag="IU325_09820"
     CDS             complement(17308..18456)
                     /gene="galK"
                     /locus_tag="IU325_09820"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP5809319.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(18460..19506)
                     /gene="galT"
                     /locus_tag="IU325_09825"
     CDS             complement(18460..19506)
                     /gene="galT"
                     /locus_tag="IU325_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HAP5809320.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESKDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDGQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(19516..20532)
                     /gene="galE"
                     /locus_tag="IU325_09830"
     CDS             complement(19516..20532)
                     /gene="galE"
                     /locus_tag="IU325_09830"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP5809321.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(20795..22267)
                     /gene="modF"
                     /locus_tag="IU325_09835"
     CDS             complement(20795..22267)
                     /gene="modF"
                     /locus_tag="IU325_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HAP5809322.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLSPGEDDTGRTT
                     AEIIQDEVKDTPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAELLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIN"
     gene            complement(22335..23123)
                     /gene="modE"
                     /locus_tag="IU325_09840"
     CDS             complement(22335..23123)
                     /gene="modE"
                     /locus_tag="IU325_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HAP5809323.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAMLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAIAQNADNQLPGIISHIERGAEQCE
                     VIMSLPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            23252..23401
                     /locus_tag="IU325_09845"
     CDS             23252..23401
                     /locus_tag="IU325_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump-associated protein, AcrZ
                     family"
                     /protein_id="HAP5809324.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            23568..24341
                     /gene="modA"
                     /locus_tag="IU325_09850"
     CDS             23568..24341
                     /gene="modA"
                     /locus_tag="IU325_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP5809325.1"
                     /translation="MARKWLNLFTGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVIAFYDYLKGPQAAEIFKRYGFTTK"
     gene            24341..25030
                     /gene="modB"
                     /locus_tag="IU325_09855"
     CDS             24341..25030
                     /gene="modB"
                     /locus_tag="IU325_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HAP5809326.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGLSFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            25033..26091
                     /gene="modC"
                     /locus_tag="IU325_09860"
     CDS             25033..26091
                     /gene="modC"
                     /locus_tag="IU325_09860"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HAP5809327.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(26092..26910)
                     /locus_tag="IU325_09865"
     CDS             complement(26092..26910)
                     /locus_tag="IU325_09865"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
                     /protein_id="HAP5809328.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLAKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIARFIYSHLI"
     gene            27065..28060
                     /locus_tag="IU325_09870"
     CDS             27065..28060
                     /locus_tag="IU325_09870"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HAP5809329.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(28101..29054)
                     /locus_tag="IU325_09875"
     CDS             complement(28101..29054)
                     /locus_tag="IU325_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP5809330.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKVGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLVKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
BASE COUNT         6990 a         7131 c         7694 g         7277 t
ORIGIN      
        1 aaatcaattt ttaattcttg aaaaaaatct gcaaaacacg ataaattgtc gtagatcaaa
       61 ttggtgagat cgtgacaatt tcgctataaa ttgatcactg tcgaaaaatg caaatttgct
      121 caataaaaac ctgtttattg taaggatttt gcggcgtaat ataattacgg gatcaatttg
      181 gtttattcat taacatattt gtaacttttt tatcattgtc ttttcctctt ttgatgaaat
      241 ggcaacgata gaggcgagca aataattttg tattggggca tgcgtgtgac cctttctaac
      301 ggggttcact ctcggagtct tcatgcgatg agcaaggagt catgatgtta gatatagtcg
      361 aactgtcgcg cttacagttt gccttgaccg cgatgtacca cttccttttt gtgccactga
      421 cgctcggtat ggcgttcctg ctggccatta tggaaacggt ctacgtcctt tccggcaaac
      481 agatttataa agatatgacc aagttctggg gcaagttgtt tggtatcaac ttcgctctgg
      541 gtgtggctac cggtctgact atggagttcc agttcgggac taactggtct tactattccc
      601 actatgtagg ggatatcttc ggtgcgccgc tggcaatcga aggtctgatg gccttcttcc
      661 tcgaatccac ctttgtaggt ctgttcttct tcggttggga tcgtctgggt aaagttcagc
      721 atatgtgtgt cacctggctg gtggcgctcg gttcaaacct gtccgcactg tggattctgg
      781 ttgcgaacgg ctggatgcaa aacccaatcg cgtccgattt caactttgaa actatgcgta
      841 tggagatggt gagcttctcc gagctggtgc ttaacccggt tgctcaggtg aaattcgttc
      901 acactgtagc gtctggttat gtgactggcg cgatgttcat cctcggtatc agcgcatggt
      961 atatgctgaa aggtcgtgac ttcgccttcg ctaaacgctc ctttgctatc gctgccagct
     1021 tcggtatggc tgccgttctg tctgttattg ttctgggtga tgaatctggt tacgaaatgg
     1081 gcgacgtgca gaaaaccaaa ctggctgcta ttgaagccga gtgggaaacg caacctgcgc
     1141 ctgctgcctt tactctgttc ggcattcctg atcaggaaga ggagacgaac aaatttgcga
     1201 tccagatccc ttacgcgctg ggtatcattg caacgcgttc cgtggatact ccagttatcg
     1261 gcctgaaaga actgatggtg cagcatgaag aacgcattcg taacgggatg aaggcgtact
     1321 ctctgctcga gcaactgcgt tctggttcta ccgaccaggc ggttcgtgac cagttcaata
     1381 gcatgaagaa agacctcggt tacggtctgc tgctgaaacg ctatacgcca aacgtggctg
     1441 atgcgactga agcgcagatt caacaggcaa ccaaagactc cattccgcgt gtagcgccgc
     1501 tgtacttcgc gttccgtatc atggtggcgt gtggcttcct gctgctggca atcatcgcgc
     1561 tctctttctg gagtgtcatc cgcaaccgca ttggcgagaa aaaatggctg ctgcgcgccg
     1621 cgctgtacgg tattccgctg ccgtggattg ctgtagaagc gggctggttt gtggctgaat
     1681 atggccgcca accgtgggct atcggtgaag tgctgccgac agctgtggcg aactcgtcac
     1741 tgaccgcagg cgatctcatc ttctcaatgg tgctgatttg cggcctgtat accctgttcc
     1801 tggtggcaga attgttctta atgttcaagt ttgcacgcct cggcccaagc agcctgaaaa
     1861 ccggtcgcta tcactttgag cagtcttcca cgactactca gccggcacgc taagacagga
     1921 gtcgtcaaat gatcgattat gaagtattgc gttttatctg gtggctgctg gttggcgttc
     1981 tgctgattgg ttttgcagtc actgacggtt tcgacatggg ggtgggcatg ctcacccgtt
     2041 tcctcggtcg taacgacacc gagcgtcgaa ttatgattaa ctccatcgca ccacactggg
     2101 acggtaacca ggtttggctg atcaccgcag gcggcgcact ctttgctgcc tggccgatgg
     2161 tctatgccgc tgcgttctcc ggcttctatg tggcgatgat cctcgtgctg gcgtctttgt
     2221 tcttccgtcc ggtcggtttt gactaccgct ccaagattga agaaacccgc tggcgtaaca
     2281 tgtgggactg gggcatcttc attggtagct tcgttccgcc gctggtgatt ggtgtagcat
     2341 tcggtaacct gttgcagggc gtaccgttca acgttgatga atatctgcgt ctgtactaca
     2401 ccggtaactt cttccagctg cttaacccgt tcggcctgct ggctggcgtg gtgagcgtag
     2461 ggatgatcat tactcagggc gcaacctatc tgcaaatgcg taccgtgggc gaactgcacc
     2521 tgcgtacccg tgcaacggct caggtggcgg cactggtgac actggtctgc ttcgcactgg
     2581 ctggcgtatg ggtgatgtac ggtatcgatg gttatgttgt gaaatcaaca atggaccatt
     2641 acgcagcctc taacccgttg aataaagaag tggttcgtga agctggcgca tggctggtta
     2701 acttcaacaa cacgccaatt ctgtgggcta ttccggcact gggtgtagtt ctgccgctgc
     2761 tgaccatcct gactgctcgt atggataaag ctgcgtgggc gtttgtgttc tcctccctga
     2821 cgctggcctg catcatcctg actgccggta tcgcaatgtt cccgtttgtg atgccgtcca
     2881 gcaccatgat gaacgcaagt ctgacaatgt gggatgcaac ttccagccag ctgacgctta
     2941 acgtcatgac ctgggttgcg gtggttctgg taccgatcat tctgctctac accgcctggt
     3001 gttactggaa aatgttcggc cgtatcacca aagaagatat tgaacgtaac acccactctc
     3061 tgtactaagt aaggagctaa aaatgtggta tttcgcatgg attctgggaa cgcttcttgc
     3121 ctgttcgttt ggggtaatca ccgcgctggc gcttgagcac gtcgaatcag gcaaagccgg
     3181 tcaagaagac atctgatgag taagattatc gcaaccttgt atgtggtaat ggacaagcgc
     3241 cccctgcggg cgctttcctt cgtgatggcg cttctgttag caggatgtat gttttgggac
     3301 ccatcacgtt tcgccgcgaa gaccagtgaa ctggaaatct ggcatggttt attgctgatg
     3361 tgggccgtct gtgctggtgt tattcacggc gtgggctttc gtccgcagaa ggttctctgg
     3421 caagggattt tttgcccttt gcttgccgat attgttctca ttgtcgggct gattttcttc
     3481 ttcttttaaa tcagaattct ctttaaaaat ttatgggccg ctccaggccc ataaattttt
     3541 acgctccctt aacttgccct cattcccaaa cctcaatcgc gcgcgtatag tagcagcgtt
     3601 taaaagctct aacttttgtt gcattaccgg gatgtaaagt gaatacaacg ctgtttcgat
     3661 ggccggttcg cgtctactat gaagataccg atgccggtgg tgtggtgtac cacgccagtt
     3721 acgtcgcttt ttatgaaaga gcacgcacag agatgctgcg tcatcatcac ttcagtcaac
     3781 aggcgctgat ggctgaacgc gttgcctttg tggtacgtaa aatgacggtg gaatattacg
     3841 cacctgcgcg gctcgacgat atgctcgaaa tacagactga aataacatca atgcgtggca
     3901 cctctttggt tttcacgcaa cgtattgtca acgccgagaa tactttgctg aatgaagcag
     3961 aggttctggt tgtttgcgtt gacccactca aaatgaagcc tcgtgcgctt cccaagtcta
     4021 ttgtcgcgga gtttaagcag tgactgacat gaatatcctt gatttgttcc tgaaggctag
     4081 ccttctggtt aaacttatca tgttgatttt gattggtttt tcaatcgcat cctgggccat
     4141 tattatccag cggacccgta ttcttaacgc agcggcgcgt gaagccgaag cgtttgaaga
     4201 taaattctgg tctggaatcg aactctctcg cctctatcaa gagagtcagg ggaaacggga
     4261 taatctgact ggttcggaac aaatctttta cagcgggttc aaagagtttg tgcgcctgca
     4321 tcgtgccaat agccatgcgc cggaagccgt agtggaaggg gcgtcgcgtg ctatgcgtat
     4381 ctccatgaac cgtgaacttg aaaatctgga aacgcacatt ccgttcctcg gtacggttgg
     4441 ctccatcagc ccgtatattg gtctgtttgg tacggtctgg gggatcatgc acgcctttat
     4501 cgccctcggg gcggtaaaac aagcaacact gcaaatggtt gcgcccggta tcgcagaagc
     4561 gttgattgcg acggcaattg gtctgttcgc cgcaattccg gcggtaatgg cttataaccg
     4621 tctcaaccag cgcgtaaaca aactggaact gaattacgac aactttatgg aagagtttac
     4681 cgcgattctg caccgccagg cgtttaccgt tagcgagagc aacaaggggt aagccatggc
     4741 cagagcgcgt ggacgaggtc gtcgcgatct caagtccgaa atcaacattg taccgttgct
     4801 ggacgtactg ctggtgctgt tgctgatctt tatggcgaca gcgcccatca tcacccagag
     4861 cgtggaggtc gatctgccag acgctactga atcacaggcg gtgagcagta acgataatcc
     4921 gccagtgatt gttgaagtgt ctggtattgg tcagtacacc gtggtggttg agaaagatcg
     4981 cctggagcgt ttaccaccag agcaggtggt ggcggaagtg tccagccgtt tcaaggccaa
     5041 cccgaaaacg gtctttctga tcggtggcgc aaaagatgtg ccttacgatg aaataattaa
     5101 agcactgaac ttgttacata gtgcgggtgt gaaatcggtt ggtttaatga cgcagcctat
     5161 ctaaacatct gcgtttccct tgcttgaaag agagcgggta acaggcgaac agtttttgga
     5221 aaccgagagt gtcaaaggca accgaacaaa acgacaagct caagcgggcg ataattattt
     5281 cagcagtgct gcatgtcatc ttatttgcgg cgctgatctg gagttcgttc gatgagaata
     5341 tagaagcttc agccggaggc ggcggtggtt catccatcga cgctgtcatg gttgattcag
     5401 gtgcggtagt tgagcagtac aaacgcatgc aaagccagga atcaagcgcg aagcgttctg
     5461 atgagcagcg caagatgaaa gaacagcagg ctgctgaaga actgcgtgag aaacaagcgg
     5521 ctgaacagga acgcctgaag caacttgaga aagagcggtt agctgctcag gaacagaaaa
     5581 agcaggctga agaagccgca aaacaggccg agttaaagca gaagcaagcg gaagaagcgg
     5641 cagcgaaagc ggcggcagat gctaaagcga aggccgaagc ggatgctaaa gctgcggaag
     5701 aagctgcgaa gaaagcggtt gcagacgcga agaaaaaagc agaagcagaa gccgccaaag
     5761 ccgcagccga agcgcagaaa aaagccgagg cagccgcggc ggcactgaag aagaaagcgg
     5821 aagcggcaga agcagctgca gctgaagcaa gaaagaaagc ggcaactgaa gctgctgaaa
     5881 aagccaaagc agaagctgag aagaaagcgg ctgctgaaaa ggctgcagct gagaagaaag
     5941 cggctgctga aaaggctgca gctgataaga aagcggcagc agagaaagct gcagccgaca
     6001 aaaaagcagc agaaaaagcg gctgctgaaa aggcagcagc tgataagaaa gcagcggcag
     6061 aaaaagccgc cgcagacaaa aaagcggcag ctgcaaaagc agcagctgaa aaagccgctg
     6121 cagcaaaagc tgccgcagag gcagatgata ttttcggtga gctaagctct ggtaagaatg
     6181 caccgaaaac ggggggaggg gcgaaaggga acaatgcttc gccagccggg agtggtaata
     6241 ctaaaaacaa tggcgcatca ggggccgata tcaataacta tgccgggcag attaaatctg
     6301 ctatcgaaag taagttctat gacgcatcgt cctatgcagg caaaacctgt acgctgcgca
     6361 taaaactggc acccgatggt atgttactgg atatcaaacc tgaaggtggc gatcccgcac
     6421 tttgtcaggc tgcgttggca gcagctaaac ttgcgaagat cccgaaacca ccaagccagg
     6481 cagtatatga agtgttcaaa aacgcgccat tggacttcaa accgtaatcg cgatgttgac
     6541 tgttccgacg gtcaacatca ggcaccggtt gccacggggt tctggtagtt ttgtgtattt
     6601 tagtttgtta acattctgct aaattatcgt gggccatcgg tccagataag ggagatatga
     6661 tgaagcaggc attacgagta gcatttggtt ttctcatact gtgggcatca gttctgcatg
     6721 ctgaagtccg cattgtgatc gacagcggtg tagattccgg tcgtcctatt ggtgttgttc
     6781 ctttccagtg ggcgggtcct ggtgcggcac ctgaagatat tggcggcatc gttgctgctg
     6841 acttgcgtaa cagcggtaaa tttaatccgt tagatcgcgc tcgtctgcca cagcagccgg
     6901 gtagtgcgca ggaagtacaa ccagctgcat ggtccgcgct gggtattgac gctgtagttg
     6961 tcggtcaggt cactccgaat ccggatggtt cttacaatgt tgcttatcaa cttgttgaca
     7021 ctggcggcgc accgggtact gtacttgctc agaactcgta caaagtgaac aagcagtggc
     7081 tgcgttatgc tggtcatacc gccagtgatg aagtgtttga aaaactgacc ggcattaaag
     7141 gtgcgttccg tacccgtatt gcctacgttg ttcagaccaa cggcggtcag ttcccgtatg
     7201 aactgcgcgt atctgactat gacggttaca accagtttgt cgttcaccgt tcaccgcagc
     7261 cgctgatgtc tccggcgtgg tcaccagacg gttctaaact ggcttatgtg accttcgaaa
     7321 gcggtcgttc cgcgctggtc atccaaacgc tggcaaatgg cgctgtacgt caggtggctt
     7381 cattcccgcg tcacaacggt gcacctgcat tctcgccaga cggcagcaaa ctggcattcg
     7441 ccttgtcgaa aaccggtagt ctgaacctgt acgtaatgga tttggcttct ggtcagatcc
     7501 gccaggtgac ggatggtcgc agtaacaata ccgaaccgac ctggttcccg gacagccaga
     7561 acctggcatt tacttctgac caggccggtc gtccacaggt ttataaagtg aatatcaacg
     7621 gcggtgcgcc acaacgtatt acctgggaag gttcgcagaa ccaggatgcg gatgtcagca
     7681 gcgacggtaa atttatggta atggtcagct ccaatggtgg gcagcagcac attgccaaac
     7741 aagatctggc aacgggaggc gtacaagttc tgtcgtccac gttcctggat gaaacgccaa
     7801 gtctggcacc taacggcact atggtaatct acagctcttc tcaggggatg ggatccgtgc
     7861 tgaatttggt ttctacagat gggcgtttca aagcgcgtct tccggcaact gatggacagg
     7921 tcaaattccc tgcctggtcg ccgtatctgt gataataatt aattgaatag taaaggaatc
     7981 attgaaatgc aactgaacaa agtgctgaaa gggctgatga ttgctctgcc tgttatggca
     8041 attgcggcat gttcttccaa caagaacgcc agcaatgacg gcagcgaagg catgctgggt
     8101 gccggcactg gtatggatgc gaacggcggc aatggcaaca tgtcttccga agagcaggct
     8161 cgtctgcaaa tgcaacagct gcagcagaac aacatcgttt acttcgatct ggacaagtac
     8221 gatatccgtt ctgacttcgc tcaaatgctg gatgcacacg caaacttcct gcgtagcaac
     8281 ccgtcttaca aagtcaccgt agaaggtcac gcggacgaac gtggtactcc ggaatacaac
     8341 atctccctgg gtgaacgtcg tgcgaacgcc gttaagatgt acctgcaggg taaaggcgtt
     8401 tctgcagacc agatctctat cgtttcttac ggtaaagaaa aacctgcagt actgggtcat
     8461 gacgaagcgg catactccaa aaaccgtcgt gcggtactgg tttactaaga gaattgcatg
     8521 agcagtaact tcagacatca actattgagt ctgtcgttac tggttggtat agcggccccc
     8581 tgggccgctt ttgctcaggc accaatcagt agtgtcggct caggctcggt cgaagaccgc
     8641 gtcactcaac ttgagcgtat ttcaaatgct cacagccagc ttttaaccca actccagcaa
     8701 caactctctg ataatcaatc cgatattgat tccctgcgtg gtcagattca ggaaaatcag
     8761 tatcaactga atcaggtcgt ggagcggcaa aagcagatcc tgttgcagat cgatagcctc
     8821 agcagcggtg gtgcagcggc gcaatcaacc agcggcgatc aaagcggtgc ggcggcatca
     8881 acgacgccga cagctgatgc tggtactgcg aatgctggcg cgccggtgaa aagcggtgat
     8941 gcaaacacgg attacaatgc agctattgcg ctggtgcagg ataaatcccg ccaggatgac
     9001 gcaatggtgg catttcagaa tttcatcaaa aattaccctg attcaactta cctgccaaac
     9061 gccaattatt ggctgggtca gttaaactac aacaagggta aaaaagatga tgcggcgtat
     9121 tattttgctt cggtagtgaa aaactatccg aagtcaccaa aggctgcaga tgcgatgttt
     9181 aaagtcggcg tcatcatgca ggacaaaggt gataccgcaa aagcgaaagc cgtataccag
     9241 caggttatca gtaaataccc tggtactgat ggcgctaaac aggcacaaaa acgtcttaac
     9301 gcgatgtaat gcataacaca cgaccagaag tcgcattatt tctggtcgtg tcgtgcgaat
     9361 cataagcagt tgagtgatct acatcgaaat ttttgttgcg ctcaagtctg aaatcagtaa
     9421 tatatgccgc cgttgccacg ggatatcaaa caaaccgaaa gcaacgaaaa agtgggtcgt
     9481 tagctcagtt ggtagagcag ttgactttta atcaattggt cgcaggttcg aatcctgcac
     9541 gacccaccac taacctagtt agttgtagta tccagcgtag tatcgggtga ttagctcagc
     9601 tgggagagca cctcccttac aaggaggggg tcggcggttc gatcccgtca tcacccacca
     9661 ccgggtcgtt agctcagttg gtagagcagt tgacttttaa tcaattggtc gcaggttcga
     9721 atcctgcacg acccaccact aattacggtg ggttcggtgg aagtagtttg tagtatccag
     9781 cgcagtatcg ggtgattagc tcagctggga gagcacctcc cttacaagga gggggtcggc
     9841 ggttcgatcc cgtcatcacc caccactcgg gtcgttagct cagttggtag agcagttgac
     9901 ttttaatcaa ttggtcgcag gttcgaatcc tgcacgaccc accaatttta acatcaaact
     9961 cagatgttaa gcgtgaagga taacgttgcg ccagcaacgg cccgaagggc gagcgaagcg
    10021 agtcatcctg cacgacccac caattttaaa gattggttcc gagtagaaat ctttcaggta
    10081 acacccgtat gggtcgttag ctcagttggt agagcagttg acttttaatc aattggtcgc
    10141 aggttcgaat cctgcacgac ccaccatcct gaatgattaa agcagcataa tcccgcaagg
    10201 ggtcgttagc tcagttggta gagcagttga cttttaatca attggtcgca ggttcgaatc
    10261 ctgcacgacc caccaatgta aaaaagcgcc ctaaaggcgc ttttttgcta ttcaggcatc
    10321 tctcaatttc aattcttaac cctgatgaca tactccgcgc ttgctgcgta aacaactcta
    10381 cgtcggagga ataagacgca aagtgtggca ttcgtcatct catacgtaat tcgagtgact
    10441 tttccccacc attcgactat cttgtttagc atataaaaca aatttcaccg ataacagcga
    10501 atattacgct catgtcggtt ttaacgttaa gcctgtaaaa cgagatggta agatgagcgt
    10561 gatgtttgat ccagacacgg cgatttatcc tttccccccg aagccgacgc cgttaagcat
    10621 tgatgaaaaa gcgtattacc gcgagaagat aaaacgtctg ctaaaagaac gtaatgcggt
    10681 gatggttgcc cattactata ccgatcccga aattcaacaa ctggcagaag aaaccggtgg
    10741 ctgtatttct gattctctgg aaatggcgcg cttcggtgca aagcatcccg cttctacgtt
    10801 gttagtcgct ggggtgagat ttatgggaga aaccgccaaa attctcagtc cggaaaaaac
    10861 aattctgatg ccgacacttc aggctgaatg ttcactggat ctcggctgcc ctgttgaaga
    10921 atttaacgca ttttgcgatg cccatcccga tcatactgtc gttgtctacg ccaacacttc
    10981 tgctgcggta aaagcgcggg cagattgggt ggtaacttca agcattgccg tcgaacttat
    11041 tgatcatctg gatagtttgg gtgaaaaaat catctgggct cccgacaaac atctggggcg
    11101 ttacgtgcaa aaacagacgg gcgcagacat tttatgctgg cagggtgcct gcattgtgca
    11161 tgatgaattt aagactcagg cgttaacccg cttgcaagaa gaatacccgg atgctgccat
    11221 actggtgcat ccagaatcac cacaagctat tgtcgatatg gcggatgcgg tcggttccac
    11281 cagtcaactg atcgctgctg cgaaaacatt gccacatcag aggcttattg tggcaaccga
    11341 tcggggtatt ttctacaaaa tgcagcaggc ggtgccagat aaagagttac tggaagcacc
    11401 aaccgcaggt gagggtgcaa cctgccgcag ctgcgcgcat tgtccgtgga tggccatgaa
    11461 tggccttcag gccatcgcag aggcattaga acaggaagga agcaatcacg aggttcatgt
    11521 tgatgaaagg ctgcgagaga gggcgctggt gccgctcaat cgtatgctgg attttgcggc
    11581 tacactacgt ggataacgaa taataaggcg taacgttacg ctttggggga aagatggatt
    11641 tttttagtgt gcagaatatc ctggtacata taccaatagg agcaggcggt tatgatctct
    11701 catggatcga agcggtaggc acgatcgctg ggttgctgtg tattggcctt gccagtatgg
    11761 agaagatcag caactacttc tttggcctga tcaacgtcac cttgtttggc attattttct
    11821 ttcagattca gctgtatgcc agcctgctat tacaggtgtt tttctttgcc gcgaatattt
    11881 acggttggta tgcgtggtcg cgacaaacca gtcagaacga ggcggagttg aaaattcgct
    11941 ggttgccatt gccgaaggcg ctcagctggt tggcggtttg cgttgtttcg attggtctga
    12001 tgacggtatt tatcaatccg gtgtttgcat ttttgacccg cgtggcagtc atgatcatgc
    12061 aagcattagg attacaggtt gcgatgcctg aactacagcc ggacgctttc ccgttctggg
    12121 attcatgcat gatggtgtta tccatcgtgg caatgattct gatgacgcgt aagtatgtgg
    12181 aaaactggct gttgtgggtg attattaacg tgattagcgt cgttattttt gcacttcagg
    12241 gcgtttacgc catgtctctg gagtacatca tcctgacctt tattgcgctt aacggcagcc
    12301 ggatgtggat caacagcgca cgcgaaagag gttcacgcgc gctgtcccat taatggtgat
    12361 gatgtgaatg gccagacacc ccttcattca ggtggcagtc tggcccatga caaggttgat
    12421 attccatctg aatagtggcg tgctcaatct gatagtgatc catcaggtag tgttggatct
    12481 gatctaacaa ggcatcgtga tcgtgtggcg ggatcacctg cacatgcagc gtcatcaccg
    12541 gcttctcgcc taccatccat acatgtacat ggtgcacatt gcggacttcc gagatttccc
    12601 ggcacatacg acgcttcagt tcggcgatat ccagcgatac cggtgcacct tcaagtaatt
    12661 cattcacgct atctttcaac agtcgccacg cgctacgcag aaccagaagc gacaccagta
    12721 ttgagagaat ggggtcagca ggtgtccagc cggtccagat aataatcaac gcggcaataa
    12781 tagccccaac cgaacccagc aggtccccga gcacatgcag tgccgctgct cgcacgttga
    12841 ggtttttctc ttcgctgccg tgatgaagta accagaaaga aagtatattt gccagcagcc
    12901 cagccacggc aattgccatc atcatgccgc cctcgaccgg acgcggagtg cggaagcgtt
    12961 ctatcgcctc ccagacaatc aaaatagtaa tcaccaccag ggcgatggca ttcacaaacg
    13021 ccgcgagcgt ggtcaatctt agccaaccga aagtgtggcg aatggtggga ggacgacggg
    13081 aaaattgcac tgcgagtagg gcgaaaagca gggcggcagt atcggtcaac atatgacccg
    13141 catcggccag caatgccaga gaaccggaaa gaaaaccacc aatgacttct acgagcataa
    13201 acccggcagt cacgccgaaa gcatacaaca agcgacgagc attattatct tctggcaggt
    13261 gtgaggaagt gtgtgagtgt gagtgcgcca tgacgagtca tcccttaatt attctcactt
    13321 aactttatga catcatactg cttttagaag ggaaaaatta aaagggagag actccgctct
    13381 cccattattg gctatttcgc agggttactg cgtggtaccg tcggttttgg tatcgacatc
    13441 attgttgatg ccatcaccag tttgtacctt tttattgata tccggacagc gaccatcttt
    13501 gcacatggtg ttcttgtgct cttcatcttt ggtcatgccg tcattgttca ttgaagaacc
    13561 atccggatgc agcattgtgc cgccagaacc ggtatttacc ccgttattgt cgacgttatt
    13621 tggcgcgaca ttttcacggg catcaggggc tacctggccc gcatcagctg cggcgtttgc
    13681 ctggccgtta ttgctttgcg ctccgctatc ggcagccagt gcggcaccgc tggcaaggct
    13741 tagagtggca gtcaaaaata gtgtggccag ttttgtcatt ttcataggat gctcctgtta
    13801 tggtcgttat gtcggataac ctcttccaac agtgcatttg caggtgaata taaggcaatg
    13861 gtttaagatt tcagccaagc aaagataccc agcagagaat cttgaaataa ttaacaaaca
    13921 aaggagttac agttagaaat tgtaggagag atctcgtttt tcgcgacaat ctggcgtttt
    13981 tcttgctaat tccaggatta atccgttcat agtgtaaaac cccgtttaca cattctgacg
    14041 gaagatatag attggaagta ttgcattcac taagataagt atggcaacac tggaacagac
    14101 atgaattatc agaacgacga tttacgcatc aaagaaatca aagagttact tcctcctgtc
    14161 gcattgctgg aaaaattccc cgctactgaa aatgccgcga atacggtcgc ccatgcccga
    14221 aaagcgatcc ataaaatcct gaaaggtaat gatgatcgcc tgttggtggt gattggtccg
    14281 tgctcaattc atgatcctgt cgcggctaaa gagtatgccg ctcgcttgct gacgctgcgt
    14341 gaagagctgc aagatgagct ggaaatcgtg atgcgcgtct attttgaaaa gccgcgtact
    14401 acggtgggct ggaaagggct gattaacgat ccgcatatgg ataacagctt ccagatcaac
    14461 gacggtctgc gtattgcccg caaattgctg ctcgatatta acgacagcgg tctgccagcg
    14521 gcgggtgaat tcctggatat gatcacccca caatatctcg ctgacctgat gagctggggc
    14581 gcaattggcg cacgtactac ggaatcgcag gtgcaccgcg aactggcatc agggctttct
    14641 tgcccggtag gcttcaaaaa tggcaccgac ggtacgatta aagtggctat cgatgccatt
    14701 aatgccgccg gtacgccgca ctgcttcctg tccgtaacga aatgggggca ttcggcgatt
    14761 gtgaatacca gcggtaacgg cgattgccat atcattctgc gcggtggtaa agagcctaac
    14821 tacagcgcga agcacgttgc tgaagtgaaa gaagggctga acaaagcagg cctgccagca
    14881 caggtgatga tcgatttcag ccatgctaac tcgtcaaaac aattcaaaaa gcagatggat
    14941 gtttgtgctg acgtttgcca gcagattgcc ggtggcgaaa aggccattat tggcgtgatg
    15001 gtggaaagcc atctggtgga aggcaatcag agcctcgaga gtggggaacc gctggcctat
    15061 ggcaagagca tcactgatgc ctgcatcggc tgggaagata ccgatgctct gttacgtcaa
    15121 ctggcgaatg cagtaaaagc gcgtcgcggg taaggtttaa ttgtcggatg cgccgtcaga
    15181 gtggcatatc cgatgaatca ccgtagccct gataagacgc gcagcgtagc atcaggcaat
    15241 gtgctccatt gttagcaacg acaaacaaaa aagccgactc acttgcagtc ggctttctca
    15301 tttttaaacg aatgacgttt acttcgcttt accctggttc gcaacggctg ctgctttcgc
    15361 cgcgatctcg tcagcattac ccagataata gcgtttcagc ggtttgaagt tctcgtcaaa
    15421 ctcataaacc agcggcacgc cggtcgggat attcagctca agaatctctt cttcgctcat
    15481 gttatcaaga tatttcacca gcgcacgtaa cgagttaccg tgtgcagcga tgattacgcg
    15541 ctcaccgctc ttcatacgcg gtagaatagt ttcattccag taagggatca cgcggtcaat
    15601 ggtcagcgcc aggctttccg tcagtggcag ttctttctcg ctcagcttcg cgtaacgtgg
    15661 atcgtgaccc ggataacgct catcatcttt agtcagttcc ggcggagtca ctgcaaaacc
    15721 acgacgccac tgtttcacct gctcgtcgcc atacttttca gcagtttccg ctttgttcag
    15781 accctgcaac gcaccgtagt gacgttcgtt cagtttccag gatttctcaa cgggcagcca
    15841 tgcctgatcc agttcgtcca gcacattcca cagggtatga atagcgcgtt tcagcacaga
    15901 ggtgtaagca aagtcaaagc tgtaaccttc ctctttcagc agcttacctg ctgcttttgc
    15961 ttcgcttacg cctttctcag acagatccac gtcgtaccaa ccggtgaaac ggttttcttt
    16021 gttccactga ctttcgccat gacgaaccag aaccagctta gttacagcca tatacttact
    16081 cctcaaatca tcttttaatg ataataattc tcattatatt gccgtgacga agcaacagca
    16141 atgcttacgc ataaccatag cgaaaatagt gtcgcagtgt aaggttgttg tgaatattga
    16201 gttgcaaata tgtcggtgtt tgctggtgat ttgaacaata tgaggataaa gccctcatga
    16261 cgagggctta acattactga gcaataaact gatattccgt caggctggaa tactcttcgc
    16321 caggacgcag gaagcagtct ggttgcggcc attcagggtg gttcgggctg tccggcagaa
    16381 attcgctttc cagagccagc ccttgccagt cggcgtaagg ttcggttccc cgcgacggtg
    16441 tgccgccgag gaagttgccg gagtagaatt gcagagccgg agcggtggtg tagaccttca
    16501 gctgcaattt ttcatctgct gaccagacat acgccgccac tttcttgcca tcgcctttgg
    16561 cctgtaacaa gaacgcgtga tcgtaacctt tcactttgcg ctgatcgtcg tcggcaagaa
    16621 actcactggc gatgattttg gcgctgcgga aatcaaaaga cgttccggcg acagatttca
    16681 ggccgtcgtg cggaatgccg ccttcatcaa ccggcagata ttcgtccgcc agaatctgca
    16741 acttgtgatt gcgcacgtca gactgctcgc cgtcaagatt gaaatagacg tgattagtca
    16801 tattcaccgg gcaaggttta tcaactgtgg cgcgataagt aatggagata cggttatcgt
    16861 cggtcagacg atattgcacc gtcgcgccga gattacccgg gaagccctga tcgccatcat
    16921 ctgaactcag ggcaaacagc acctgacgat cattctggtt cacaatctgc cagcgacgtt
    16981 tgtcgaaccc ttccgggccg ccgtgcagct ggttaacgcc ctgacttggt gaaagcgtca
    17041 cggtttcacc gtcaaaggta taacggctat tggcgatgcg gttggcataa cgaccaatag
    17101 aggcccccag aaatgcggcc tgatcctgat agcattccgg gctggcgcag ccaagcagcg
    17161 cctctcggac gctgccatcg gaaagcggaa tacgggcgga aagtaaagtc gaaccccagt
    17221 ccatcagcgt gactaccatc cctgcgttgt tacgcaaagt taacagtcgg tacggctgac
    17281 catcgggtgc cagtgcggga gtttcgttca gcactgtcct gctccttgtg atggtttaca
    17341 aacgtaaaaa gtctctttaa tacctgtttt tgcttcatat tgttcagcga cagcttgctg
    17401 tacggcaggc accagctctt ccgggatcag cgcgacgata cagccgccaa atccgccgcc
    17461 ggtcatgcgt acgccacctt tgtcgccaat cacagctttg acgatttcta ccagagtgtc
    17521 aatttgcgga acggtgattt cgaaatcatc gcgcatagag gcatgagact ccgccatcaa
    17581 ctcgcccata cgtttcaggt cgccttgctc cagcgcgctg gcagcttcaa cggtgcgggc
    17641 gttttcagtc aggatatgac gcacgcgttt cgccacgatt gggtccagtt catgcgcaac
    17701 agcgttgaac tcttcaatgg tgacatcgcg cagggctggc tgctggaaga aacgcgcacc
    17761 ggtttcgcac tgttcacgac gggtgttgta ttcgctgcca accagggtac gtttgaagtt
    17821 actgttgata atgacgacag ccacaccttt cggcatggag actgctttgg tacccagtga
    17881 acggcaatcg atcagcaagg catgatcttt cttgccgagt gcggaaatta gctgatccat
    17941 gatcccgcag ttacagccaa caaactggtt ttctgcttcc tggccgttaa gcgcgatttg
    18001 tgcgccgtcc agcggcagat gataaagctg ctgcaatacg gttccgactg cgacttccag
    18061 tgaggcggaa gaacttaacc cggcaccctg cggcacgttg ccgctaatca ccatgtccac
    18121 gccgccgaag ctgttgttac gcagttgcag atgtttcacc acgccacgaa cgtagttagc
    18181 ccattgatag ttttcatgtg cgacaatggg cgcatcaagg gaaaactcgt cgagctgatt
    18241 ttcataatcg gctgccatta cgcgaacttt acggtcatcg cgtggcgcac aactgatcac
    18301 ggtttgataa tcaatcgcgc agggcagaac gaaaccgtcg ttgtagtcgg tgtgttcacc
    18361 aatcaaattc acgcggccag gcgcctgaat ggtgtgagtg gcagggtagc caaatgcgtt
    18421 ggcaaacaga gattgtgttt tttctttcag actcatttct tacactccgg attcgcgaaa
    18481 atggatatcg ctgactgcgc gcaaacgctc tgctgcctgt tctgcggtca ggtctcgctg
    18541 ggtctctgcc agcatttcat aaccaaccat aaatttacgt acggtggcgg agcgcaacag
    18601 aggcggataa aagtgcgcgt gcagctgcca gtgttgattc tcttcgccat taaacggtgc
    18661 gccgtgccag cccatagagt aggggaagga gcactggaag aggttgtcat aacgactggt
    18721 cagctttttc aacgccagcg ccaaatcgct gcgctggccg tcggtcaaat cggtgatccg
    18781 caaaacgtgg gctttgggca gtagtagcgt ttcgaacggc caggcagccc agtaaggcac
    18841 tacggctaac cagtgttcgg tttcgacaac ggtacggcta ccgtctgcca gctcgcgctg
    18901 aacataatcc accagcattg gtgatttctg ttcggcaaaa tattcttttt gcaggcggtc
    18961 ttcacgctca gcttcgttag gcaggaagct atttgcccaa atctgtccgt gcggatgcgg
    19021 gttagagcag cccatcgccg cacctttgtt ttcaaagacc tgcacccatg ggtatgtttt
    19081 ccccagttct gcggtttgct cctgccaggt tttgacgatt tccgtcaatg ccgcaacgct
    19141 cagttctggc aacgttttac tgtgatccgg tgaaaagcag atcacccggc tggtaccgcg
    19201 cgcgctctgg caacgcatca gcggatcttt gctttctggc gcatctggcg tgtcagacat
    19261 caaggccgca aagtcattag tgaaaacgta agtcccggtg taatcggggt ttttatcgcc
    19321 tgtcacccgc acattacctg cgcagaggaa gcaatctgga tcatgcgcag gtaacacctg
    19381 tttggctggc gtttcctgcg ccccctgcca ggggcgctta gcgcggtgcg gtgaaaccag
    19441 aatccattgc ccggtgagcg ggttgtagcg gcgatgtgga tgatcaacgg gattaaattg
    19501 cgtcatggtc gttccttaat cgggatatcc ctgtggatgg cgtgactgcc agtgccaggt
    19561 gtcctgcgcc atttcatcga gtgtgcgcgt cacgcgccag ttcagttcac ggtcggcttt
    19621 gctggcgtcc gcccagtagg ccggaaggtc gccctcgcga cgcggtgcaa aatgatagtt
    19681 aaccggtttg ccgcaggctt tgctgaaggc attaaccacg tccagcacgc tgctgcctac
    19741 gccagcgccg aggttgtaga tgtgtacgcc tggcttgttc gccagtttct ccatcgccac
    19801 gacgtgaccg tccgccagat ccattacgtg gatgtaatcg cgtacgccag taccgtcttc
    19861 ggtcggataa tcgttaccaa aaatcgccag cgagtcgcga cggcctacag caacctgggc
    19921 gatgtatggc atcaggttat tcggaatgcc ttgcggatct tcgcccatat cgcccgacgg
    19981 atgtgcgcca accgggttga agtagcgcag cagggcaatg ctccagtccg gctgggcttt
    20041 ttgcagatcg gtgaggatct gttcaaccat cagtttgctt ttgccgtaag ggctttgcgg
    20101 tgtgccggtc gggaagcttt caacgtatgg aattttgggc tgatcgccat aaacggtggc
    20161 agaggagcta aaaataaagt ttttgacgtt cgcggcgcgc atggcgctaa tcaggcggag
    20221 agtaccgttg acattgttgt cgtaatattc cagcggtttt tgtaccgatt cgccaacggc
    20281 tttcagcccg gcgaagtgga tcacggtgtc gatagcatga tcgtgcagga tctcggtcat
    20341 caacgcttcg ttacgaatat cgccttcaac aaacgttgga tgtttgccgc ctaaacgctc
    20401 gataacgggc agtacgctgc gcttactgtt acagaggtta tcaagaatga tgacatcatg
    20461 accgttttgc agtaattgca cacaggtatg acttccaatg taaccgctac caccggtaac
    20521 cagaactctc ataattcgct ccattaggct tatggtatga aataaccata gcataacaaa
    20581 gatgcgaaaa gtgtgacatg gaataaatta gtggaatcgt ttacacaaga atttagccgt
    20641 tttttatgcg cgattaagtg attataaaac agagggttta tgaatgattg cgctttttat
    20701 ctgaaaaaaa agacgcggtt tcatgcctgg atgcgtcgaa ccgttggccg gagagggcgc
    20761 ttaggccgcc tccggcacgg tcagcactac cgactcaatt tatttttgtc agcacatagc
    20821 gatagagtcc gccgtctggc acgaactcaa gacgatgggt aatacaggca ggcgcatctt
    20881 cagcgtggtg cgaaacaaac aacaattgcg tttcaccttc gctaatcagc acatcaacaa
    20941 accggcggat aagctggcga ttgagcggat caagcccctg tagtggttca tcgagaataa
    21001 gcaacgtcgg atgtttcacc agcgcgcgga cgatcagcgc caggcgttgt tgcccccagg
    21061 aaagactatg gaacggagcg tcagccgtgc gtttatcaat gccgagaata tccagccact
    21121 gctgcaccag tttttgctgg cgatccgaaa cggcctgata aatgccaatc gaatcaaaat
    21181 agccagaaag aatcacatta cgcacagtag tactgacccg gtaatccaga tgcaaactac
    21241 tgctgacgta accgatatgc tttttgatat cccagatggt ttcgccgctg ccgcgacgtc
    21301 gtccgaaaag cgtcaaatcg ttgctgtaac cttgcggatg atcgccagta atcaggctta
    21361 ataacgtcga ttttcccgcg ccatttggcc cgacaatttg ccagtgttca cctggattca
    21421 cctgccagct aaggttatta agaatggggc gatcattata agaaaccacg ccattgttca
    21481 gcacaatgcg cggttcgttg gcgggtaagg cgtgacgtgc tgaaggttca tccggctccg
    21541 gcagttgtac accttcaagc tgttcgctat gcgccagttg cgcgacgagt gcttgctgga
    21601 gcagttcctc tttagcgcca gtttccgcta acgtacaatc cgccagcacg ccagcaaact
    21661 ggacaaattc tggtatctca tcgaagcgat tgagcaccag taccagagta ataccggact
    21721 gatgtaacga ggcgagtagc tcagccagct gctgacgtga ggcaacatcc aggccatcga
    21781 acggttcatc aaggatcaac aagtcaggct ccgacatcag cgcctgacac agcagggttt
    21841 ttcgcgtctc gccagtggaa aggtatttaa agcgtcggtc gaggagggcg gtaataccga
    21901 actgctgcgc cagttgcatg caacgcggag tatcctttac ttcatcctga atgatctcag
    21961 ccgtggtacg cccggtgtca tcttcgccag gactaagcat atcggtgtta ttccgctgcc
    22021 attcgtcgct gacgagcttt tgcaattgct cgaaggagag acgagtgatg tgggaaaact
    22081 ggctttgccg ttcacctttc aaaagcggaa gttcccccgc cagcgcgcgg gccagggccg
    22141 atttcccgct tccattcgaa ccgacaaacg cccaactatc acccgcgttt aacgttagct
    22201 gaggcaattg cagcgttttt gtgtcgctaa gacgaaacgt gccttgcaaa atttgcaacg
    22261 atgacatttt atatcccatt ttgtgcagcg attactgaca gggatacgtg tttcataaca
    22321 aattgtcaac acgcttagca cagcgtggcg ataatcacgc tgtcggcatt aaagtaggcc
    22381 gtgacattct gtccttgctg aagagaagtc gcttcattta ccggcactgt ggcgcacagt
    22441 gtttgcccgt cgggtagcga cattattact tcgcactgct ctgcgccacg ctcaatatga
    22501 ctaataatac ccggtaattg gttgtcggcg ttttgcgcga tcgcttcgtc ctgagtaatg
    22561 cctacccacg gcgcttttag caatatcaac acttctttgc cttcatccag ccccagacgc
    22621 gcgccgcttt gtgcggtaat tgcgactttc aggcgtgttt ttccatcagc cagtaagaca
    22681 tcaacatgct gttgaacgtc atcatgatcg cgggcggtga tggtaccgaa ccactggtta
    22741 cgggcgctgg tttgcagtga aaaacgtgag atcgcggcca gcaggctgtt cagcggcagg
    22801 gcgtcatcgt cacttaacac atcaaaggct ttttgctgga tttgcgccag taagtcatag
    22861 agctgaatca gtcgctgacc atagcgagtc agcatcgcgc cgccgccacc tttaccgcct
    22921 gttgcgcgct cgaccagaat atgctcactt aactgattca tttcgttaat ggcatcccag
    22981 gcgcttttat agctgatacc ggcatctttc gctccctggc taatggaacc ggaaagcgca
    23041 atgtgtttta gtagcgaaat gcggcgcggg tcggcgaata atttttgttg gagcttaagg
    23101 gtgagaagga tttcggcctg cataacaata tcctggcaaa agtcttattg tgacggaaaa
    23161 cgaacgccac gcaaagctga ccgcacaaaa ggggagtgct tttctgtgct tagcggttag
    23221 aatagtctca tgactatatc tggagttgac catgttagag ttattaaaaa gtctggtatt
    23281 cgccgtaatc atggtacctg tcgtgatggc catcatcctg ggtctgattt acggtcttgg
    23341 tgaagtattc aacatctttt ctggtgttgg taaaaaagac cagcccggac aaaatcattg
    23401 attccctgaa tgcccgctta gtcgggcatt ttctttttct caacttcctg cttttcctgc
    23461 cgatattttt tctcatctac ctcacaaagg ttagaaataa ctgctgggaa aattccgaac
    23521 ttgccgttat attgacgcct acataacgtt agattaaggg gttaccaatg gctcgtaaat
    23581 ggttgaactt gtttaccggg gcggcactct ctttcgctgt tgctggcaat gcactggcag
    23641 atgaagggaa aatcacggtg ttcgccgccg catcactgac taacgcaatg caggacattg
    23701 ctacgcagta taaaaaagag aaaggcgtgg atgtggtttc ttctttcgct tcgtcatcta
    23761 ctttggcccg tcagattgaa gcgggtgcgc ctgcggatct gtttatttct gccgatcaga
    23821 aatggatgga ttatgcggtt gataaaaaag cgatcgatac ggcttcgcgc cagacactgc
    23881 tcggcaatag cctggtcgtt gtagcaccga aagccagcga gcagaaagat ttcaccatcg
    23941 acagcaaaac taactggact tcgctgctga atggcggtcg cctcgcggtt ggcgatccgg
    24001 aacatgtccc tgctggcatt tatgcaaaag aagcactgca aaaactgggc gcatgggata
    24061 cgctctcccc gaaactggct ccggcggaag atgttcgtgg cgcgctggcg ttggtcgaac
    24121 gcaatgaagc gccgctgggt attgtttacg gttctgacgc agttgccagc aaaggggtaa
    24181 aagtggttgc caccttcccg gaagattcac ataaaaaagt ggaatatccg gttgctgttg
    24241 tggaagggca taataatgcg acagtgatag cattttatga ttatctgaag gggccgcagg
    24301 ctgcggaaat ctttaaacgt tacggattta caaccaagta atgatactga ccgatccaga
    24361 atggcaggca gttttattaa gcctgaaagt ttcttccctg gctgtgctgt ttagcctgcc
    24421 gtttgggatc ttttttgcct ggttactggt acgttgcacg tttccgggca aagctctgct
    24481 cgatagcgta ctgcatctac cgctggtgtt accgcccgtg gtcgtcggtt acttattatt
    24541 agtttcgatg ggacggcgcg gatttatcgg tgaacgtctg tatgactggt ttggtctttc
    24601 tttcgcgttt agctggcgcg gcgcggttct cgctgccgcc gtcatgtcgt ttccgctgat
    24661 ggtgcgggca attcgtctgg cgctggaagg ggttgatgtc aaactggaac aggccgcaag
    24721 aacactgggt gccgggcgct ggcgcgtttt ctttactatc acgttaccgc tgaccttacc
    24781 gggcattatt gttggtacgg tactggcttt tgctcgttct ctcggtgagt ttggtgcaac
    24841 catcaccttt gtgtcgaaca ttcctggtga aacgcgaacc attccttctg ccatgtatac
    24901 cctgatccag actcccggcg gggaaagtgg cgcggcgaga ctgtgcatta tttctattgc
    24961 actggcgatg atctccctgt tgatttcaga atggctggcc agaatcagcc gtgaacgggc
    25021 ggggcgctaa tcatgctgga actgaatttt tcccagacgt tgggcaacca ttgcctgacc
    25081 attaatgaaa cgctgcccgc caatggcatc actgctatct ttggcgtctc cggtgccgga
    25141 aaaacttcgc tgattaacgc catcagtgga ctgacgcgcc cgcaaaaagg gcggattgtc
    25201 ctcaatgggc gggtactaaa tgatgccgaa aaaggtatct gcctgacgcc agaaaagcgt
    25261 cgcgttggct atgtttttca ggatgcacgg ctgttcccgc attacaaagt gcgtggcaat
    25321 ctgcgctacg gcatgtcgaa aagtatggtc gatcagttcg ataagctggt ggcgctttta
    25381 ggcattgaac cgttgcttga ccgtttacca ggcagcctgt ccggaggcga aaaacagcgc
    25441 gtggcgattg gtcgggcttt gctgacagca ccggaattgc tgttgctgga tgaaccgctg
    25501 gcgtcactgg atattccgcg taaacgcgaa ctgttgcctt atctgcaacg gctgacacgg
    25561 gaaatcaaca ttccgatgtt gtatgtcagc cattcgctgg atgagatcct ccatctggca
    25621 gacagagtga tggtactgga aaacggtcag gtgaaagcct ttggcgcgct ggaggaagtg
    25681 tggggcagta gcgtgatgaa tccgtggctg ccgaaagagc aacaaagtag cattctgaaa
    25741 gtgacggtgc ttgagcatca tccgcattac gcgatgaccg cgctggcgct gggcgatcag
    25801 catttgtggg tcaataagct ggacgaaccg ctgcaagctg cgctacgtat ccgcattcag
    25861 gcttccgatg tttctctggt tttacaaccg ccgcagcaaa ccagcattcg taacgtattg
    25921 cgggcaaaag ttgttaatag ttatgacgac aacggccagg tggaagtgga gctggaagtc
    25981 ggcggtaaaa cgctgtgggc gcgtatcagc ccgtgggcca gggatgaact ggcgatcaaa
    26041 ccaggcctgt ggctgtacgc gcaaattaaa agtgtgtcga taaccgcctg attaaatcag
    26101 gtggctataa atgaaccggg caatgctgtc ggtggtgtta tcaccaatca caatgttggc
    26161 gcgcgctttt accgcatcat cggcgttgcc catcgccacg cctgtaccag cggcttccag
    26221 catactgata tcattaaagt tatcgccgaa tgccacgacg ttttccatcg accaaccttg
    26281 tgcctcaacc catttcgcca aacgtttacc tttgctgttg ccgccgcgtg caatatcaac
    26341 ctgatcgtgc caggaccatt cacactcaag tcccagttca tgttcgacat gcttaccaaa
    26401 atgctgcaat tgcggcaggt catcgtgcgt cagggcgaac ttccatacgg cgttaacttg
    26461 ttgcgccgtt tcagccagag aagcgacttg tgtgaaagtc ggacgctgtt ccggcggcag
    26521 ggtttgcgcc cagttagacg tgcgaatgac atgcccggtc gggtgctcat agaccattgc
    26581 atcatcgaca tacatcagac cgtgaatgtg gtgttcattc agcatctcaa tgagttgcag
    26641 ggctttatta acgggcattg ggtccgcttc cagcactgtt tttgcatgat aatcatacaa
    26701 ataggtgcca ttacagcaaa tagcaggtgt atccagcgcc agcgcctgat aaaaaggatg
    26761 aatagcgacg tgatggcgac ctgtgacgat gattaattga tagcctgctt cgcgagcgcg
    26821 ggccagggct tctatcgatg atggaagcaa ggtctttttc ggggtcaata aggtgccgtc
    26881 taagtcgaga gcaatcacgc gtgtggtcat ggcgtattcc agattaaggt taagaatttt
    26941 cttctgcgcg aatggtacac cgataccact atcggacaaa attctgcgtt ttaattcagc
    27001 attcaccgcc aaaagcgact aattttagct gttacagtca gttgctaaat gcaaaggagc
    27061 attcatgaag caaacagttt atatcgccag ccctgagagc cagcaaattc acgtctggaa
    27121 cctgaatcat gaaggcgcac tgacgctgac acaggttgtc gatgtgccgg ggcaggtgca
    27181 gccgatggtg gtcagcccgg acaaacgtta tctctatgtt ggtgtgcgcc ctgagtttcg
    27241 cgtcctggcg tatcgtatcg ccccggacga tggcgcgctg acctttgctg cagagtctgc
    27301 gctaccgggt agcccgacgc atatttccac cgatcaccag gggcagtttg tctttgtcgg
    27361 ttcttacaat gcgggtaacg tgagcgtaac gcgtctggaa gatggcctgc cagtgggcgt
    27421 cgtcgatgtg gtcgaggggc tggatggttg ccattccgcc aatatctcac cggacaaccg
    27481 tacgctgtgg gttccggcat taaagcagga tcgcatttgt ctgtttacgg tcagcgatga
    27541 tggtcatctg gtggcacagg accctgcgga agtgactacc gttgaagggg ccggcccgcg
    27601 tcatatggta ttccatccaa acgaacaata tgcgtattgc gtcaatgagt taaacagctc
    27661 agtggatgtc tgggaactga aagatccgca cggcaatatc gaatgtgtcc agacgctgga
    27721 tatgatgccg gaaaacttct ccgacacccg ttgggcggct gatattcata tcaccccgga
    27781 tggtcgtcat ttatacgcct gcgaccgtac cgccagcctg attaccgttt tcagcgtttc
    27841 ggaagatggc agcgtgttga gtaaagaagg cttccagcca acggaaaccc agccgcgcgg
    27901 cttcaatgtt gatcacagcg gcaagtatct gattgccgcc gggcaaaaat ctcaccacat
    27961 ctcggtatac gaaattgttg gcgagcaggg gctactgcat gaaaaaggcc gctatgcggt
    28021 cgggcaggga ccaatgtggg tggtggttaa cgcacactaa ccgctgattt acccggcgca
    28081 gtctctcctg cgccggtgta ttaacctatc tcctgtaacg catgtctctg gcgttcgacg
    28141 atattggtcc acaaattgtc tttatcgtca gtccacaaat taatcagcaa ggcaaaaaag
    28201 cgttcagctg ccggagaaag cacggcatct ttgcggcgaa taatccccaa tgtccgacgt
    28261 ataacgggtt ccaccagtgg gataccaata atcgaagaat agggcgcatg gggcattgcc
    28321 agaccaggaa gcgccgaaat ccccagtcct gcctccacca gtcctaatga cgtcgaaaga
    28381 tgacgcactt cgtaaaacca gtccagcttc cagggcttgt cggctagctg ttgttctatc
    28441 aacagtcggt tgccgctgga ggagcgtacg ccaatcattt tgtaacccac taactcctgc
    28501 cattctacaa gctgcttttt gaccaatgga tggtcacgcc gacaggccag cacgaacggc
    28561 tcgttgacca gtggggtaaa atcgatggat gaatttgtga cgttgttcat gtttatgcca
    28621 aagtctgatt cgttacacag caccgattcc atgcaattat tcgtgccttg ttccagaatc
    28681 cgcactttaa tattgggata cagctcatta aatttaccga ttgccagcgg taaaaaataa
    28741 aatactgcgg tcggaataca cgctaatgtc accataccac gatgataagc attcatatca
    28801 cgaatattaa aaagcgtttc atcgaatttc tttattaatt cccgcgcctc cgggagcaat
    28861 ctttttccta cttttgttaa cgtaaccttg cgagttgtac gctcaaatag ttggatatgt
    28921 aaatcttcct ccatcttttt tattcttcgc gttaatgcag gttgcgtaat attgagtaat
    28981 ttagcggcat tgttaaatga actggactct gccagtatga caaatgcctt catacttgat
    29041 aattcatgct tcattattac tccggaaaat ggaagcgtcg attttgggtg gc
//
LOCUS       DACBVI010000046        28758 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.046, whole genome shotgun sequence.
ACCESSION   DACBVI010000046 DACBVI010000000
VERSION     DACBVI010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.046"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            238..1008
                     /locus_tag="IU325_09880"
     CDS             238..1008
                     /locus_tag="IU325_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP5809331.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            999..1472
                     /locus_tag="IU325_09885"
     CDS             999..1472
                     /locus_tag="IU325_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP5809332.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSESLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1580..2119
                     /gene="dnaT"
                     /locus_tag="IU325_09890"
     CDS             1580..2119
                     /gene="dnaT"
                     /locus_tag="IU325_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HAP5809333.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELTSFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2122..2859
                     /gene="dnaC"
                     /locus_tag="IU325_09895"
     CDS             2122..2859
                     /gene="dnaC"
                     /locus_tag="IU325_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001731874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HAP5809334.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2908..3402
                     /gene="yjjA"
                     /locus_tag="IU325_09900"
     CDS             2908..3402
                     /gene="yjjA"
                     /locus_tag="IU325_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HAP5809335.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQMLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3656..5947
                     /gene="opgB"
                     /locus_tag="IU325_09905"
     CDS             3656..5947
                     /gene="opgB"
                     /locus_tag="IU325_09905"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HAP5809336.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRHR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHIDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6086..7108)
                     /locus_tag="IU325_09910"
     CDS             complement(6086..7108)
                     /locus_tag="IU325_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP5809337.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7247..8161
                     /locus_tag="IU325_09915"
     CDS             7247..8161
                     /locus_tag="IU325_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP5809338.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8376..9737
                     /locus_tag="IU325_09920"
     CDS             8376..9737
                     /locus_tag="IU325_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5809339.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNAHRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9786..11441)
                     /locus_tag="IU325_09925"
     CDS             complement(9786..11441)
                     /locus_tag="IU325_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAP5809340.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQDDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQRETSAVVKNVTPATPRKMAVADSGENWETF"
     gene            11818..13968
                     /gene="btsT"
                     /locus_tag="IU325_09930"
     CDS             11818..13968
                     /gene="btsT"
                     /locus_tag="IU325_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HAP5809341.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPLIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            14018..14221
                     /locus_tag="IU325_09935"
     CDS             14018..14221
                     /locus_tag="IU325_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenoprotein"
                     /protein_id="HAP5809342.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            14232..15188
                     /locus_tag="IU325_09940"
     CDS             14232..15188
                     /locus_tag="IU325_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HAP5809343.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(15222..15524)
                     /locus_tag="IU325_09945"
     CDS             complement(15222..15524)
                     /locus_tag="IU325_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system, antitoxin component"
                     /protein_id="HAP5809344.1"
                     /translation="MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDE
                     QWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKGYQTRLNAILREAMLRDQNKK"
     gene            complement(15511..15798)
                     /locus_tag="IU325_09950"
     CDS             complement(15511..15798)
                     /locus_tag="IU325_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HAP5809345.1"
                     /translation="MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIEN
                     GEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIISARKADRKERNRYEHG"
     gene            complement(15997..16693)
                     /locus_tag="IU325_09955"
                     /pseudo
     CDS             complement(15997..16693)
                     /locus_tag="IU325_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            17008..18240
                     /gene="mdtM"
                     /locus_tag="IU325_09960"
     CDS             17008..18240
                     /gene="mdtM"
                     /locus_tag="IU325_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HAP5809346.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRVGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPLQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEEQ"
     gene            18281..19561
                     /locus_tag="IU325_09965"
     CDS             18281..19561
                     /locus_tag="IU325_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HAP5809347.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKVDINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            19677..20828
                     /locus_tag="IU325_09970"
     CDS             19677..20828
                     /locus_tag="IU325_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HAP5809348.1"
                     /translation="MSLVTDLPAIFDQFSEARQTGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLKMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            20838..21605
                     /locus_tag="IU325_09975"
     CDS             20838..21605
                     /locus_tag="IU325_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809349.1"
                     /translation="MAYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLEPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVIPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQRF
                     THMLESHLGMPVQTHPDAQFAGAIGAAVIGQRQRKRA"
     gene            21602..21859
                     /locus_tag="IU325_09980"
     CDS             21602..21859
                     /locus_tag="IU325_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HAP5809350.1"
                     /translation="MKEYLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGHTESVYCQQDGGWRCIAHYGISPR"
     gene            21924..22784
                     /locus_tag="IU325_09985"
     CDS             21924..22784
                     /locus_tag="IU325_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809351.1"
                     /translation="MTKSISLSKRIFVIVILFVIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDLIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQESPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDKALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEMSLTKGSRGLSHNIKQAEGVAMDASGNIYIVSEPNR
                     FYRFTPQSSH"
     gene            22852..24030
                     /locus_tag="IU325_09990"
     CDS             22852..24030
                     /locus_tag="IU325_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HAP5809352.1"
                     /translation="MPSSTHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASALASGLLILAMAWL
                     PPFILVLLIRVLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMMLIALTLLMPSKKHAITPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASDSPLLLIISSLGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(24043..24597)
                     /gene="kptA"
                     /locus_tag="IU325_09995"
     CDS             complement(24043..24597)
                     /gene="kptA"
                     /locus_tag="IU325_09995"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HAP5809353.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GLPFWQAENGVWLTSTVAVEFLEW"
     gene            24847..25530
                     /locus_tag="IU325_10000"
     CDS             24847..25530
                     /locus_tag="IU325_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809354.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            25527..25988
                     /locus_tag="IU325_10005"
     CDS             25527..25988
                     /locus_tag="IU325_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809355.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            26001..27173
                     /gene="iadA"
                     /locus_tag="IU325_10010"
     CDS             26001..27173
                     /gene="iadA"
                     /locus_tag="IU325_10010"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP5809356.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            27238..28149
                     /gene="hypT"
                     /locus_tag="IU325_10015"
                     /gene_synonym="qseD"
     CDS             27238..28149
                     /gene="hypT"
                     /locus_tag="IU325_10015"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HAP5809357.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(28142..28534)
                     /gene="iraD"
                     /locus_tag="IU325_10020"
     CDS             complement(28142..28534)
                     /gene="iraD"
                     /locus_tag="IU325_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HAP5809358.1"
                     /translation="MMRQSRQAVLPEISGNKTSSLRKSICSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYSHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(28531..28614)
                     /locus_tag="IU325_10025"
     CDS             complement(28531..28614)
                     /locus_tag="IU325_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809359.1"
                     /translation="MENEHQYSGARCSGQAAYIAKRQECAK"
BASE COUNT         6660 a         7493 c         7280 g         7325 t
ORIGIN      
        1 atctattatc ctgattcgta aaggccaaac aataaattca ttatgatgat aatattcatg
       61 tgatagtttg tttgcggcga gagataattc gctttttatc accgagcttc tcccgataac
      121 caggattaat cagcgcaatt ttgatcttaa cctaccgcaa aacaaccgtg cttcatgata
      181 caaatggatt ctcattatct gaacaatacg cagcacgtct atgacaaagg gcgagttatg
      241 caaactgagc aacagcgagc cgtaacgcgg ctttgtatcc agtgtggatt atttcttttg
      301 caacatggtg cggaaagcgc gttggttgat gagctttcct cacgactggg tcgggcgctg
      361 ggaatggaca gcgttgaaag ttctatctct tcgaacgcca tagtgctgac aaccattaaa
      421 gatgggcaat gcctgacatc gacacgtaaa aatcacgatc gcggcattaa tatgcatgtg
      481 gtgactgaag tccagcacat tgtgattctg gcggagcacc atctgctgga ttacaaaggc
      541 gtagagaaac gatttagcca aattcagcca ttacgttacc caagatggct ggtagcctta
      601 atggttggcc tttcttgtgc ctgtttctgt aaactcaata aaggtggctg ggatggtgcc
      661 gtcatcacct tctttgccag tacggccgcg atgtatatcc gccagctgct ggcacaacgt
      721 catcttcatc cacaaatcaa cttttgcctt accgctttcg ccgccaccac catttccgga
      781 ttgcttttgc aactccccac tttcagcaat acccccacca ttgcgatggc cgccagcgtt
      841 ctgctgctgg tgccgggatt tccgttgata aatgccgtcg ccgatatgtt taaaggccac
      901 atcaataccg gactggcacg ctgggcgatc gccagtctgc tgacactggc tacctgcgtc
      961 ggcgtggtga tggcactgac gatttggggg ctacgcggat gggtgtgatc gagtttctgt
     1021 ttgcattggc gcaggatatg atcctcgccg ccattccagc ggtcggcttt gcgatggtgt
     1081 tcaacgttcc cgttcgggca ttacgctggt gtgcgctgct tggcgcgatt ggtcatggtt
     1141 cacgaatgat cttaatgacc agcgggctga atattgagtg gtcaaccttt atggcttcta
     1201 tgctggtcgg caccattggt attcaatggt cgcgctggta tctggcgcat ccgaaagtgt
     1261 ttaccgtggc ggctgttatc cccatgttcc ctggcatatc ggcttatacc gcaatgattt
     1321 cggcggtaaa aatcagccag ttaggctaca gcgagtcatt gatgattacc ctgttaacca
     1381 actttcttac agcttcatcg attgttggtg cattatccat cggtctttcc attcctggat
     1441 tatggttgta ccgcaagcgc cctcgcgtat aaaattgcct cgcttaaagc acacggatga
     1501 gagacagcct cctctcctcc gtgtgttact ataaaagtta tctcccttct cgttcatcgt
     1561 tccatatttg agaaacagta tgtcttccag agttttgacc ccggacgtcg ttggtattga
     1621 cgccctggta cacgatcacc aaaccgttct ggcaaaagct gaaggcggtg tggttgccgt
     1681 atttgctaac aatgccccgg cgttttatgc cgtcacgcct gcacgcctgg ctgaactgct
     1741 ggcgctggaa gaaaagctgg cgcgtccggg aagcgatgtc gctctggacg atcaactcta
     1801 tcaggaaccg caagctgctc ccgttgctgt acccatgggg aaattcgcca tgtatccgga
     1861 ctggcaaccc gatgccgact ttatccgcct ggcggcgcta tggggcgtgg cactaagaga
     1921 gccggtgacc accgaagaac tgacctcatt cattgcctac tggcaggcgg aaggtaaagt
     1981 ctttcaccat gtacagtggc aacaaaaact ggcgcgcagc ctgcaaatcg gtcgtgccag
     2041 caacggcgga ctgccgaaac gagatgtgaa tacggtcagt gaacctgaca gccaaattcc
     2101 accaggattc agagggtaac gatgaaaaac gttggcgacc tgatgcaacg cctgcaaaaa
     2161 atgatgcctg cccatatcaa acctgcattc aaaacgggtg aagaactact ggcctggcag
     2221 aaagaacaag gagcgatccg ctccgccgct ctcgaacgtg aaaatcgggc gatgaaaatg
     2281 cagcgcacct ttaaccgctc cggtattcgt ccactgcatc agaactgctc ctttgagaac
     2341 tatcgcgttg agtgtgaagg gcagatgaat gcgttaagca aagcgcgcca gtacgtcgaa
     2401 gagtttgacg gcaacatcgc cagcttcatc ttttctggta agccaggaac cggcaaaaac
     2461 catctggcgg cggcaatctg caacgagctg ctgctacgcg gtaaatccgt attgatcatc
     2521 accgtggccg atattatgtc ggcgatgaaa gataccttca ggaatagcgg taccagcgaa
     2581 gaacaactgc ttaacgatct gagcaacgtt gatctgctgg tgatcgatga gatcggcgtg
     2641 cagaccgaat cgaaatacga aaaagtgatc atcaaccaga tcgtcgatcg ccgctcttct
     2701 tccaaacgcc caaccgggat gctgaccaac agtaatatgg aagagatgac caagttgctg
     2761 ggcgaacgcg tgatggaccg tatgcgcctg ggtaacagtt tgtgggtgat cttcaactgg
     2821 gatagctacc gtagccgggt aacaggtaaa gagtattaat ccccgtttct ggcggtatac
     2881 tgcgacacaa ttccaccttg agtaatgatg atgaaaactg ttaaacatct tctgtgctgt
     2941 gccattgccg ccagcgcatt aatttccacc ggggtgcatg ctgcgtcctg gaaagatgcg
     3001 ctctccagcg ccgccagcga acttggcaac caaaacagca cgacacagga aggcggttgg
     3061 tcgctcgcgt cattaactaa cttgcttagc agcggaaacc aggccttaag cgcagataac
     3121 atgaacaatg ccgcaggcat tctgcaatac tgcgcgaagc aaaagctggc ttcggtaacc
     3181 gatgccgaaa acatcaagaa ccagatgctg gaaaagctgg gcctgaacag tgaagagcaa
     3241 aaagaagaca ccaactatct ggacggcatt cagggtttgc tgaaaaccaa agatggtcag
     3301 caactcaatc tggataacat cggaacgact ccgctggcag aaaaggtgaa aaccaaagcc
     3361 tgcgatctgg tgttaaagca ggggctgaac ttcatttcct gaaccgcgaa acgccgcgtt
     3421 ttagctgtta cccggctgac gcggctctct cctgcccttt tccctgctcc tggacggttt
     3481 tccccctgtt acacgggaag cgaccgcgat caaaaaagtc tgtttataaa ctaattccta
     3541 acaacatcgc atttttttga cactagaatt gcagcaatat ggcgacgcca ttacattgtg
     3601 cttctcaaaa agcttaaacg ttggctaaac ggcccggttt agtgaggatc gcccgttgtc
     3661 agaactactc tctttcgccc tttttctcgc ctctgtgctg atttacgcat ggaaagcggg
     3721 acgtaacacc tggtggtttg cagccacatt aacggtgctg gggctatttg tcgttttaaa
     3781 tatcaccctg tttgccagcg actattttac tggcgatggt attaacgacg cggttctcta
     3841 taccttaacc aacagcctga ccggtgctgg cgtcagcaaa tacatcctgc cgggtatcgg
     3901 cattgtgctg gggctgacag cggtgttcgg tgcgctgggc tggatcctgc gccgtcatcg
     3961 ccatcatccg caccattttg gttacagcct gctggcgctc ttactggcgc tgggttcggt
     4021 ggacgccagc ccggcatttc gtcagataac ggaactggtg aaatcccagt cacgcgatgg
     4081 cgacccggac tttgcggctt attataaaga gccgtcgaaa actatccctg acccgaaact
     4141 caacctggtt tatatctacg gcgaaagtct cgagcggacc tattttgata acgaagcttt
     4201 cccggatctc acgcctgaac tgggcgcgtt gaaaaatgaa ggcctggatt tcagccacac
     4261 gcagcagctg ccaggaacgg attacacgat tgcgggcatg gtggcttctc agtgcggcat
     4321 accgctgttt gccccctttg aaggcaacgc ctccgcctct gtctccagct tcttcccgca
     4381 gaacatctgt ctgggcgata tcctgaaaaa ctcgggttat cagaactatt tcgtgcaggg
     4441 cgcgaatctg cgttttgccg gtaaagatgt gttcctgaag tcgcatggct tcgaccactt
     4501 atacggctca gaagagctga aaagcgtggt ggccgacccg cactatcgca acgactgggg
     4561 attctacgac gataccgttc tcgatgaagc gtggaaaaag tttgaagagc tttcccgctc
     4621 aggtcagcga ttctcactgt ttaccctgac agtcgatacc catcacccgg atggttttat
     4681 ctcgcgtacc tgtaaccgca aaaaatatga ttttgacggt aagccgaacc agtcattcag
     4741 cgcggtaagt tgcagccagg agaatatcgc ggcgtttatc aacaaaatca aagcgtcacc
     4801 gtggtttaaa gataccgtca tcgtcgtctc ttctgaccat ttagcgatga acaacacggc
     4861 gtggaaatac ctcaataagc aggatcgcaa taacctgttt tttgtcattc gtggcgacaa
     4921 gccgcagcaa gagacgctgg cagtgaagcg taacacgatg gataacggcg cgacggtgct
     4981 ggacattctc ggtggcgata actatctcgg acttggtcgt agcagtttat ccgggcagtc
     5041 aatgtcggaa atcttcctca atatcaaaga gaaaacattg gcgtggaagc cggatatcat
     5101 ccgcctgtgg aaattcccta aagagatgaa agagttcacc atcgaccagc agaaaaacat
     5161 gattgccttc tcgggtagcc atttccgttt gccgctgctg ttgcgggttt cagacaaacg
     5221 cgtggaaccg ctgccggaaa gcgaatactc agcaccgctg cgtttccagc tggccgattt
     5281 cgctccacgc gacaatttcg tctgggttga ccgttgctat aagatggcac aactctgggc
     5341 tccggaactg gcactctcca ccgactggtg tgtctcgcaa gggcagcttg gcggtcagca
     5401 aattgttcag catattgaca aaacaacatg gcagggcaaa acggcattta aagatacggt
     5461 catcgacatg gcgcgctata aaggcaatgt cgatacgctg aaaattgttg ataacgatat
     5521 tcgctacaaa gccgacagtt tcatcttcaa cgtcgccggt gcgccagaag aggtgaaaca
     5581 gtttagtggg atttcccgtc cagagtcgtg gggccgctgg tccaacgcgc agctgggcga
     5641 tgaagtaaaa atcgagtaca agcatccgct gccaaagaaa tttgacctgg tgattaccgc
     5701 caaagcatac ggcaataacg ccagccgtcc tattccggta cgcgtaggca atgaagaaca
     5761 aacccttgtg ctgggcaatg aagtgaccac caccacactg catttcgata acccaaccga
     5821 tgccgacaca ctggtaattg tgccgccgga acctgtctca accaacgaag ggaatatcct
     5881 cggacactcg ccgcgtaagc tcgggatcgg catggtggaa attaaagtgg tggaacgtga
     5941 aggataattc ctgactgatg tgtaggtcgc ataaggtgcg acctacacac cttctgcggc
     6001 ctacaaaaca tcattgagaa acagaaaaaa acagcgccca acacttcccc ggacgctgca
     6061 tggcaacttt aataatctcg ccttatcaga aagtaattac acccttaatc aactcacgat
     6121 tgttaatcac atcgcgctcg taggtttctg ccagcgtggc gaacggatag cgatgggtta
     6181 acatcatgtc ggcagtgatt tttccttccg ccatcagtcg accgacttta gcaaaatctt
     6241 ccggcgtggc gttgcggctg cccatcatcg tcgtttcttt tttatggaat tccggatcgg
     6301 agaactgcaa ctcaccttta aacaggccga caaataccac cgtgccgccg tgacgaatca
     6361 aattcacggt gttattcatc gcatgttgat tacctgtcgc gtcgatcact ttctgcgcca
     6421 gcgaaccacc aaactgcgcc cgcagctgcg cgtcaaaatc ttcggctgac gggtccagta
     6481 caggtaattc cagacgcgtt gccacatgtt cacggcgcgc cggactggta tccgccacca
     6541 ccacctgtgc gccatcggct ttagcgattg ccgccgcgcc cagaccgatt ggccccgccc
     6601 cgaccaccag cacctgctcg ccgggagcaa tggctgcgcg acgcaccgca tgagcgctaa
     6661 tagcgaaagg ttcaatcaat gctgccgcct gcgggtcaat accgtctgcg ggcaaaatgt
     6721 tcgccaccgg cacgctcaaa tactcactaa aaccgccatc ctgatgcacg ccaatgactg
     6781 aaattttttc acagcaattg gtacgcccgc ttttacacgc cgggcattgc tgacaggcaa
     6841 cataagggat cacagcaact tgctgaccat ttttaagatc agcaatattt ttacccagcc
     6901 caacaatctc cccacatatt tcatggccta aaacacgtgg ataactaaaa aatggttgat
     6961 ttccacccca ggcatgaata tcggtaccgc aaatcccgac agacttaatt tttattaatg
     7021 cttcattgtc acccggaata ggtatctcgc gttgtttcca gactaattct ttcggctgct
     7081 ggcaaattaa aacattcatc gtagacatga ttcggctctc cattttttgt tgccttagat
     7141 atcaatgaaa atgaaaaatg gcagcggaac ggaaaatctg ttttgtgaaa acacacgcat
     7201 aaaaatgttt taaatcgggt tttaatggac tcacaaggag gaaaatatga gtcgttcaca
     7261 aaatttacgc cacaatgtga ttaaccaggt cattgatgat atggcgcggg gtcatatccc
     7321 ttccccactt ccatcgcaaa gtgcgctggc ggaaatgtac aacatcagcc gcaccacagt
     7381 gcgtcatatt ctcagccatt tacgcgaatg cggcgtcttg acgcaggttg gaaacgacta
     7441 tgttattgcg cgtaaacctg accacgatga cggtttcgcc tgtactaccg cttcaatgag
     7501 tgaacaaaac aaagtgtttg agcaggcttt tttcacgatg ataaaccagc gccagttacg
     7561 cccaggggaa actttttctg aactgcaact ggctcgggcc gcgggtgtca gcccggtggt
     7621 tgtaagagaa tatcttttaa aattcggccg ttataatctt attcaaagcg aaaagcgcgg
     7681 ccagtggagc atgaagcaat tcgatcaatc ctacgccgag cagctgtttg agctacgcga
     7741 aatgctcgaa acacattcac ttcagcactt tctcaacctg cctgatcatg atccccgctg
     7801 gctgcaggcg aaaaccatgc tcgaacgtca tcggctactg cgtgacaaca tcggcaatag
     7861 ctttcgcatg ttctcgcaac ttgacagaga ttttcactcc ttactgcttt cagctgccga
     7921 caatatcttt tttgatcaat cgcttgagat catctcagtc atctttcatt ttcactatca
     7981 atgggacgaa agcgatctta aacagcgcaa tatcattgcc gtcgatgagc atatgaccat
     8041 tctcagcgct ctgatttgcc gcagtgacct tgatgccacc cttgcgctcc gtaaccattt
     8101 gaattcagct aaacaatcaa tgattcgatc cattaatgag aacacgcgtt atgctcatta
     8161 agtacggatt aaaaacagca aactattagc gggtgcacgg ttttatcaaa agataaatac
     8221 cgggaaattc ccgcttacct atgctcacaa tcagtcgact aaaaatgttt ttgtgagcgg
     8281 tagtaaagtc ctaaaacttt aacctgccgt taatgtcaac cttgcgtaat gcacaattac
     8341 gtaaacaatt aaaattataa atacaggagt ccggcgtgga aaaagaaaat atcaccctcg
     8401 atccgcgttc ttcatttact ccatcttcgt cggcagatat tcccgtgcca ccagatggat
     8461 tagttcaacg cagtacccga attaaacgca ttcaaaccac cgccatgttg ttattatttt
     8521 ttgcggcggt aatcaattat ctcgaccgca gttcgctgtc ggtagcaaat ttaacgattc
     8581 gtgaagaatt gggattaagt gccaccgaaa tcggcgcttt gctctccgtg ttttcactcg
     8641 cttacgggat tgcgcaactt ccttgcggcc cactattgga tcgtaaaggc ccacgcctga
     8701 tgctgggact ggggatgttc ttctggtcac tgttccaggc aatgtctggc atggtgcaca
     8761 gctttacgca gttcgtgttg gtgcgtatcg gtatggggat tggtgaagcg ccgatgaacc
     8821 catgcggtgt aaaagtcatt aacgactggt tcaacatcaa agagcgcgga cgcccgatgg
     8881 gcttcttcaa cgcagcttct accattggcg ttgccgtaag cccaccgatt ctggcggcga
     8941 tgatgctggt gatgggctgg cgtgggatgt ttattaccat tggtgtgctg gggatttttc
     9001 tcgccattgg ctggtatatg ctctatcgca accgcgagca cgtagaactg actgccgttg
     9061 aacaagctta tctcaatgca ggtagcgtca atgcccaccg agatccgctc agttttgccg
     9121 aatggcgcag cctgttccgt aaccgcacaa tgtggggaat gatgctcgga ttcagtggca
     9181 tcaactacac tgcgtggctg tatctggcct ggcttcctgg ttacctgcaa acagcctata
     9241 acctggattt aaaaagcaca gggttgatgg cggctatccc tttcctgttt ggggctgccg
     9301 ggatgctggt caacggttac gttactgact ggctggtcaa agggggaatg gctccgatta
     9361 aaagccgtaa gatctgcatt attgccggga tgttctgttc tgccgccttt acgctggtcg
     9421 taccgcaagc gacaacatcc atgacggcgg ttctgctgat tggcatggca ctgttctgta
     9481 ttcactttgc cggaacatcc tgctggggct tgatccacgt cgcagttgct tctcgcatga
     9541 cggcgtcggt gggcagtatc cagaactttg ccagcttcat ctgcgcctct tttgcgccga
     9601 tcattactgg ttttattgtt gataccaccc actcattccg tctggcacta atcatctgcg
     9661 gttgcgtcac cgcagcggga gcactggcgt acatcttcct ggttcgtcag ccgatcaacg
     9721 acccgcgtaa agattaaact ccctatgccc gataacattt tgcttatcgg gcatttttat
     9781 ggcgattaaa acgtttccca gttctccccg ctatctgcca ctgccatttt acgcggcgta
     9841 gctggcgtca cgttttttac cacagccgat gtttcacgct gctgttgctg aatccggaac
     9901 actgccacag cttcggtcag gcgactggcc tgctcttcca gcgcggcggc ggcagcggca
     9961 gattcttcca ccagcgcagc gttctgttga gttacccggt ccatctcagc aaccgctaag
    10021 ccaacctgat cgataccacg gctctgctca tcagaagcag aagcaatttc gcccataatg
    10081 tccgtcacgc gggtcacggc actgacaatc tccgccattg tttcccctgc gctttcgacc
    10141 agcgtagagc caacatctac tttccccacc gagtcttcaa tcaggctttt aatttcacga
    10201 gccgcctgag cgctgcgctg agccagatta cgcacttcac ccgcgaccac cgcaaaaccg
    10261 cgcccttgct cacccgcgcg cgctgcttca accgccgcgt tcaaagccag aatattggtc
    10321 tggaaggcaa tgccgtcaat tacgctgata atatcggcga ttttctgcga actggtggag
    10381 atatcgcgca tggtctgtac tacgttatcc accactttgc cgccgcgttg cgccgtttca
    10441 gaagcactta acgccagatg gctggcctga cgcgcattct cggcgttctg tttcaccgtt
    10501 gcggtcagtt gctccatgct ggctgccgtc tcttccagcg aagcggcctg ttgctcggtg
    10561 cgcgaagaga gatcgttatt gccggtggcg atttcgctgg caccgctata gatggcattg
    10621 gccccgttgc gcacatcacc gacggtacgc atcagctctc cctgcatatg gcgcaaactc
    10681 tctgccagtt gccccatctc attagagcca tccacctcaa tcggtttcac cagatcgccg
    10741 cctgcaatat gacgaatgct gtcaatcagg cgattcattg gcgctaccag cgaggcttta
    10801 ataccgaacc agacggcgaa gatgaccgcc agtacgacga tcatcacgcc caccagaatc
    10861 cacatcgcct ggctgtagga ggcattgtta tcgctgacgg cgatatcata gagccgatcg
    10921 tcttgctcca tgtaagctac atactgcttc tcgaaaccgt cctgatatcc ctgggtcggc
    10981 tgatcaaaga actcgttgat tttgcctgca cctaatagtt ggatcagctc cgccagcgca
    11041 ttgtgataaa tatcgtaatt acgtttgatc tctgccgctg cggcggtgct ctgacgcggg
    11101 tcacgcggca acgcttcgta atccgcccag tttttttccg cctgtttcag cgaaatactg
    11161 gcactctgca tcagctcagc aacggttgaa ccgctaccaa tattattctg atccatcatg
    11221 tagcggatac ccgcgcggtt gagggtgtta cgcgtctgca acaacgcgac ccagctgcca
    11281 ttcagcgtgg attgctgctg gcgaatggtt tgtaaaacag tgaaattttc tttgtcattc
    11341 tttaaggcat taaagaacag accgcctgat gtcagttgta aaaggccaaa aacggccaaa
    11401 accagcagta agctggtcac aattttgata cgttttaaca tagtttctct ttcctgtgga
    11461 ccgatgcaag attttcggcc tggaaaggaa aaactttatg aatatcccgc ggagattacg
    11521 ccgagtaatt ttattcacac tctgaattta aaaagccatt atttacataa attattcaca
    11581 atataaatat gtgatatgaa tcacatattt atcgtcactt aaacgacgcc tttgccgctc
    11641 aaccgcaaaa ctgaccgctt acatccctaa aataaccact cagttattta ccttacttta
    11701 cgcgcgcgta actctggcaa catcactaca ggatagcggt caatttacct cctcaaacgc
    11761 aacgcaaacc tagaacggct tcggccaact attaatcaat acatgccagg ttttactatg
    11821 gatactaaaa agatattcaa gcacataccc tgggtgattc tcggaatcat cggtgcattc
    11881 tgcctcgcgg tagttgcatt acgtcggggg gagcacgtca gcgccctgtg gatcgtggtc
    11941 gcctctgtat cggtgtatct ggtggcgtat cgctactaca gtctgtacat cgcccagaag
    12001 gtgatgaaac tcgaccccac gcgcgcgacg cctgcggtta ttaacaacga cggtctgaac
    12061 tacgttccga ccaaccgtta cgtgttgttt ggtcaccact tcgccgctat cgccggtgct
    12121 ggtccgctgg tgggtccggt tctcgccgcg cagatgggct acctgcctgg cacgctgtgg
    12181 ctgctggcgg gggtcgtgct ggccggtgcg gttcaggact ttatggtgct gtttatctcc
    12241 tctcgccgta atggcgcatc tcttggtgag atgatcaaag aagagatggg accagtaccg
    12301 gggactatcg cgctgtttgg ctgtttctta atcatgatca tcatcctcgc cgtcctggcg
    12361 ctgattgtgg ttaaagccct ggccgaaagt ccgtggggtg tcttcaccgt ttgctcaacc
    12421 gtaccgattg cgctgtttat gggtatctac atgcgcttta tccgtccggg gcgtgtgggt
    12481 gaagtctctg tcattggtat cgtgctgctg gttgcctcta tctacttcgg tggcgtgatt
    12541 gctcacgatc cgtactgggg tccggcactg acctttaaag acaccaccat taccttcgcg
    12601 ctgattggct atgcgtttgt ttccgcactg ctgccggtgt ggctgatcct cgcaccgcgc
    12661 gactatctgg caaccttcct gaaaatcggc gttatcgtcg gcctggcgct gggtatcgtg
    12721 gtgctgaacc cggaactgaa aatgcctgcg atgacccagt acattgacgg tactggcccg
    12781 ctgtggaaag gcgctctgtt cccgttcctg ttcatcacca tcgcctgtgg tgcggtatct
    12841 ggcttccacg cgctgatctc ttccggtacg acgccgaaac tgctggctaa cgaaaccgac
    12901 gcgcgtttca ttggctacgg cgcaatgctg atggagtcct tcgtggcgat tatggcgctg
    12961 gttgctgcgt ccatcatcga accgggtctt tacttcgcga tgaatacccc gcctgcgggc
    13021 cttggcatca ccatgcctaa cctgcatgaa atgggtggcg agaacgcgcc gctcatcatg
    13081 gcgcagctga aagacgttac cgcacacgcg gcggcgaccg tcagctcctg gggcttcgtg
    13141 atttcgccag agcagatcct gcaaaccgcg aaagacattg gtgagccttc tgtcctgaac
    13201 cgtgcaggtg gtgcgccgac gctggcggta ggtatcgctc atgtgttcca caaagtgctg
    13261 ccgatggctg acatgggctt ctggtatcac ttcggtattc tgttcgaagc cctgttcatc
    13321 ctgaccgcgc tggatgcggg tacccgttct ggccgcttta tgctgcaaga cctgctgggt
    13381 aacttcatcc cgttcctgaa aaaaaccgac tctctggttg ccggtatcat cggtactgcg
    13441 ggctgtgtgg gtctgtgggg ctacctgctg tatcagggcg tggtcgatcc gctgggcggc
    13501 gttaagagcc tgtggccgct gttcggtatc tccaaccaga tgctggcagc cgtagcgctg
    13561 gtactgggca ccgttgtgct gattaagatg aagcgcaccc aatacatctg ggtaactgtt
    13621 gttccggctg tatggctgct tatctgcacc acctgggcgc tgggtctgaa actgttcagc
    13681 accaacccgc agatggaagg cttcttctac atggcaagcc agtacaaaga gaagattgct
    13741 aacggtactg acctgacggc gcagcagatt gccaacatga accacatcgt tgtgaacaac
    13801 tacaccaacg cgggcctgag tattctgttc ctgattgtgg tgtacagcat catcttctac
    13861 ggtttcaaaa cctggcttgc ggtgcgtaac agcgacaaac gtactgacaa agaaacaccg
    13921 tacgttccaa tcccggaagg cggcgtgaag atctcttcgc accactaacc gtgtttagcc
    13981 ccgcttcggc ggggctttgt tcttatcaga gtgaactatg tttggtaact taggacaggc
    14041 aaaaaaatat ctcggccagg cggcaaagat gttgattggc attccagact acgacaacta
    14101 cgtcgagcat atgaagacca accatcccga caagccgtac atgagctatg aagaattctt
    14161 ccgcgaacgc cagaacgcgc gctacggcgg cgatggtaaa ggcggtatgc gctgttgtta
    14221 atggagagag catgaacccg attgcagtta ccctactcac cggtttttta ggcgcgggaa
    14281 aaaccaccct gctgcgccat attcttaacg aacaacacgg ctacaagatt gccgtgattg
    14341 aaaacgaatt cggcgaagtc tccgttgatg atcaactaat tggcgaccgc gccacgcaga
    14401 tcaaaacgct gaccaacggc tgcatctgct gttcgcgctc caacgagctg gaagacgcac
    14461 tactcgacct gctggataat ctcgacaagg gcaatattca gtttgaccgt ctggtcattg
    14521 aatgcaccgg catggccgat cccggcccga ttattcagac ctttttctcc catgagattt
    14581 tatgccagcg ttacctgctg gacggtgtga ttgcgctggt ggatgcggta catgccgacg
    14641 agcagatgaa ccagttcacc atcgcccagt cccaggttgg ctacgccgac cgtattctgc
    14701 tgaccaaaac cgacgtcgca ggcgaagcag aaaaactgcg tgaacgcctg gcgcgcatca
    14761 acgcccgtgc accggtctac accgtcaccc acggcgacat cgacctgggc ctgctgttca
    14821 acaccaacgg ttttatgctg gaagagaacg tcgtcagcac caaaccgcgt ttccacttta
    14881 tcgcggataa acaaaacgat atttcgtcga ttgtggtgga actggattac ccggtagata
    14941 tcagcgaagt ttcccgggtg atggaaaacc tgcttctgga atcggcagat aaactgctgc
    15001 gttacaaagg gatgctgtgg attgacggcg aacctaaccg cctgctgttc cagggcgtcc
    15061 agcgcctcta cagcgccgac tgggaccgcc cgtggggcga tgaaaaacca catagcacaa
    15121 tggtgtttat cgggattcag ttgccggagg aagagattcg ggcagcgttt gcggggttga
    15181 ggaagtaatt ttggatgatg gccgccatgt ggcggcctga attatttttt gttctgatcg
    15241 cgcagcatcg cttcacgtaa aattgcgttc aggcgagttt gatagccttt acctggtcgc
    15301 ttcaaccatt ccatcacatc agcatcaata cgcaccgaag cctgagtttt caatggccga
    15361 taaaacttac cgcgttcggc gttagaccat tgctcatcgc tggaaggtgg gatatcactg
    15421 taatcaatat cttcgtcgga tttattcgtc agcgccttca gttcggcctc atgctgcaca
    15481 tcgagtgtag aggaggtccc tcgtttatgt ttaaccatgc tcatagcgat tcctctcttt
    15541 acgatctgct tttcgtgcac tgatgattcg aataatttca tttccgcttt cgaaacggat
    15601 ggtatgcgca accagaataa ccacgatgcc gtaaaccagg ccgattgtct gccagcgata
    15661 ctcaccgttt tctatgcgtt cttgctgtga caaatgttgt ggatcatcaa acaataacac
    15721 cgcatcttca aagcggatgc catgtttcac ccgattactt ttggccttgt tttcatccca
    15781 ttcaaactcc attggcataa ttgccatccc taattgtata tacaaaaatg tatctacaat
    15841 tattctgcaa ttatagcgtt gatgttggaa atagcatcac gtttcgaata atcaaccatg
    15901 aaattgacaa tgagtaagtt ggacttgatt catccaatag aatttctcgc ctgacatggc
    15961 acactaaaca cctcgctgtc gggtaatgac tccaacttac tgatagtgtt ttatgttcag
    16021 ataatgcccg atgaccttgt catgcagctc caccgatttt gagaacgaca gtgacttccg
    16081 tcccagcctt gccagatgtt gtctcagatt cagattatgt cgctcaatgc gctgagtgta
    16141 acgcttgctg ataacgtgca gctttccctt caggcgtgat tcatacagcg gccagccatc
    16201 cgtcatctat actacgacct caaaggccga cagcaggctc agaagacgct ccagtgtggc
    16261 cagagtgcgt tcaccgaaga cgtggccaca accgtcctcc gtatcctgtc atacgcgtaa
    16321 aacagccagc gctgacgtga tttagcaccg acgtagcccc aatgttcgtc catttcagcg
    16381 cagataatca catcactgcc cggttgtatg cgcgaggtta ccgactgcgg cctgagtttt
    16441 ttaagtgacg taaaaccgtg ttgaggccaa cgcccataat gcgtgcactg gcgcgacatc
    16501 cgacgccatt catggccata tcaatgattt tctggtgcgt accgggctga gaggcggtgt
    16561 aagtgaactg cagttgccat gttttacggc aatgagagca gagatagcgc tgatgtccgg
    16621 cagtgctttt gccgttacgc accacgcctt cagtagcgga gcaggaagga catctgatgg
    16681 aaatggaagc cacgcaagca ccttaaaatc accatcatac actaaatcag taagttggca
    16741 gcattaccaa attttaatta cacttaaggt gtatattttc tatgcaagcc atcaattaaa
    16801 gaggtgtaat gtgctgatga ctatttgtaa tcgttataca ttctgacctg gagtcagaag
    16861 gtatttctct gtctgtgtat tcgcaggcag tgtggttgat tacatgaaat tcagtacatt
    16921 tgtccttctc gcctgccttt cgtcattacc gacggtattg aatttcgttt tccccattgg
    16981 ggttctccgg acaaggagtt gtttgttatg ccacgttttt ttgcccgcca tgccgccacg
    17041 ctgtttttcc cgatggcgtt gattttgtat gactttgctg cgtatctgtc gacggatctg
    17101 atccagccag ggatcattaa tgtggtccgt gattttaatg ccgatgtcag tctggccccg
    17161 gctgccgtca gtctctatct cgctggaggc atggcattgc agtggctgct ggggccgctt
    17221 tccgacaggg ttggccgcag gccggtgctg attaccgggg cgctaatatt tacccttgcc
    17281 tgcgctgcga caatgttcac aacgtctatg acacagtttc ttatcgcgcg tgcaattcag
    17341 ggcaccagta tctgttttat tgccaccgtt ggttatgtca cggtgcagga ggcgttcgga
    17401 cagacaaaag ggatcaagtt gatggcgatt atcacctcca tcgtactgat tgcgccgatt
    17461 attggcccgc tttccggcgc agctctgatg cactttgtgc actggaaagt cctttttgcc
    17521 atcattgcgg ttatgggttt tatctcattt gttggcttac tgctggcgat gccagagacg
    17581 gtgaagcgcg gcgcggttcc atttagcgcc aaaagcgtct tgcgcgattt tcgtaatgtc
    17641 ttttgcaatc ggctgttcct ctttggcgca gcaaccatct ctttaagcta tatccccatg
    17701 atgagttggg tggctgtctc gccggtgatc ctgatcgacg cgggcggctt aacaacttcg
    17761 cagttcgcct ggacacaggt tccggtgttc ggcgcggtga ttgtcgcgaa tgccatcgtg
    17821 gcgcgtttcg ttaaagatcc gaccgaaccg cgctttatct ggcgtgccgt accccttcaa
    17881 ctggttggcc tcgcgctgtt gattatcggc aatctgctgt caccgcacgt ctggctgtgg
    17941 tcggtgctgg gcaccagtct gtatgctttc gggattggtt tgattttccc gaccttattc
    18001 cgctttacgc tgttttccaa taacttaccg aaagggaccg tctccgcatc gctgaatatg
    18061 gtgatcctga tggtgatgtc tgtctcggtc gaaatcggcc ggtggctgtg gtttaacggc
    18121 ggtcgcttgc cgtttcatct gttagccgtc gtggcgggcg ttatcgtcgt tttcaccctg
    18181 gcgggattgc ttaatcgcgt gcgccaacat caggctgccg agctggcaga ggagcagtga
    18241 tttttgcgcg atcctgccgt caggctctat tcttaacgtt atgaataaac tcattgaact
    18301 cagacgcgcc aaaatgttgg cgctctcttt actgcttatc gccgctgcta cctttgtcgt
    18361 tacgctgttt ttgccgccca atttttgggt gagcggcgtg aaggcgattg ctgaagcggc
    18421 gatggtcggc gcgctggcgg actggtttgc ggtggtggcg ctgtttcgcc gcgtgccgat
    18481 tccgatcatt tctcgccata cggcgattat cccgcgtaat aaagaccgga ttggcgaaaa
    18541 tctcggccag ttcgtgcagg aaaaatttct tgatactcaa tccctggtgg cattgattcg
    18601 acgccacgaa ccggcgttgc tgattggcaa ctggtttagc cagccagaaa acgcccgccg
    18661 cgttggtcag catctgttgc agatcatgag cggttttctc gaacttaccg atgatgcgcg
    18721 tattcagcgc ctgcttaagc gcgcggtcca tcgggcgatt gataaggtcg atctttccgg
    18781 caccagtgcg ttgatgctgg agagtatgac caaaaacgat cgtcatcagg tgctgctgga
    18841 tacgctgatc gcacagttga tcgcccttct ccagcgcgat aaatcgcgca agtttatcgc
    18901 ccagcaaatt gttcgctggc tggagagcga gcatccactg aaagccaaaa ttctccccac
    18961 cgaatggttg ggcgaacata gcgcggagtt ggtttctgac gcggtgaatt ctttgcttga
    19021 tgatattagt cgcgatcgtg cgcatcagat ccgccatgcg tttgatcgcg ccaccttcgc
    19081 cctgatcgac aagctgaaaa acgatccgga aatggcagcg cgagccgatg ccgtaaaaag
    19141 ctatctgaaa gaagatgaag cttttaatcg ctatctcagt gaattgtggg gggatttacg
    19201 ggaatggctg aaagtggata tcaacagtga agattctcgt gtgaaagaac gcatcgcacg
    19261 agcgggtcaa tggtttggcg aaacgttaat tgccgatgat gccttgcggg cgtcgttaaa
    19321 tggtcatctt gaacaagccg cgcaccgcgt cgcgcctgag ttttccgcat tcctgacgcg
    19381 ccacatcagc gatacggtaa aaagctggga tgcgcgggat atgtcgcggc aaatagagtt
    19441 aaatatcggc aaagatctgc agtttattcg tgtcaacggt acgctggttg gcggttgtat
    19501 tgggctaatt ttatatttgc tgtcgcagct cccggccttg ttccccctcg gcaattttta
    19561 gaaatccatg aaaaactaag tggctaaccg cgagagagat caaataaacc atactaaaag
    19621 aatggtaata atgtggcctc ttttcattat ctcccgtggt acggggaagg aaaatcatgt
    19681 cacttgtcac cgatctaccc gccattttcg atcagttctc tgaagctcgc cagacaggct
    19741 ttctcaccgt catggatctc aaggagcgcg gcattccgct ggttggcact tactgcacct
    19801 ttatgccgca agagatcccg atggcagccg gtgcggttgt ggtttcgctc tgttccacct
    19861 ctgatgaaac cattgaagaa gcggagaaag atctgccgcg caacctctgc ccgctgatta
    19921 aaagcagcta cggcttcggc aaaaccgata aatgccccta cttctacttt tcggatctgg
    19981 tggtcggtga aaccacctgc gacggcaaaa agaaaatgta tgaatacatg gcggagttta
    20041 agcctgttca tgtgatgcaa ttgcccaaca gcgttaagga cgatgcctcg cgtgcgttat
    20101 ggaaagccga gatgctgcgc ttgcaaaaaa cggtagaaga acgttttggg cacgagatta
    20161 gcgaagatgc tctgcgcgat gccattgcgc tgaaaaaccg cgaacgtcgc gcactggcta
    20221 atttttatca tcttgggcag ttaaatcctc cggcgcttag cggcagcgac attctgaaag
    20281 tggtttacgg cgcaaccttc cggttcgata aagaggcgtt gatcaatgaa ctggatgcaa
    20341 tgaccgcccg cgttcgtcag cagtgggaag aaggccagcg actggacccg cgtccgcgca
    20401 ttttaatcac cggctgcccg attggcggcg cagcagaaaa agtggtgcgc gcgattgaag
    20461 agaatggcgg ctgggttgtc ggttatgaaa actgcaccgg ggcgaaagcg accgagcaat
    20521 gcgtggcaga aacgggcgat gtctacgacg cgctggcgga taaatatctg gcgattggct
    20581 gctcctgtgt ttcgccgaac gatcagcgcc tgaaaatgct cagccagatg gtggaggaat
    20641 atcaggtcga tggcgtagtt gatgtgattt tgcaggcgtg ccatacctac gcggtggaat
    20701 cgctggcgat taaacgtcat gtgcgccagc agcacaacat tccttatatc gctattgaaa
    20761 cagactactc cacctcggat gtcgggcagc tcagtacccg tgtcgcggcc tttattgaga
    20821 tgctgtaagg agtggcagtg gcatattcga ttggcattga ttccggctca accgccacca
    20881 aagggatctt actggcagac ggcgtgatta cgcgccgttt cctcgttcca acaccctttc
    20941 gcccggcaac agcaattact gaagcctggg aaactctgcg cgaagggtta gagacaacgc
    21001 cgtttctgac gctcaccggc tacggacggc aactggtgga ttttgccgat aaacaagtga
    21061 cggaaatctc ctgtcacggg ctgggcgcac ggtttcttga gccagcaacg cgcgcggtaa
    21121 tcgacatcgg tggtcaggac agcaaagtga ttcagcttga tgatgacggt aacctgtgcg
    21181 atttcctgat gaatgacaaa tgcgcggcgg gcaccgggcg tttcctggag gtgatctcgc
    21241 gcacgcttgg caccagcgtc gagcaactcg acagcattac cgaaaatgtc ataccgcacg
    21301 ccatcacgag tatgtgcaca gtgtttgctg aatcagaagt gatcagcctg cgctctgcgg
    21361 gcgtcgcgcc agaagcgatt ctcgccggag tgattaacgc gatggcgcgg cggagcgcca
    21421 atttcattgc tcgtctctcc tgtgaagcgc cgattctgtt tactggtggc gttagccact
    21481 gtcaaaggtt tacccacatg ctggaaagcc atctgggaat gccggtacaa actcaccctg
    21541 atgcgcaatt cgctggtgca attggcgcgg cggtgatcgg tcagcggcag agaaaacgcg
    21601 catgaaagag tatctgtttt tatttcactc cacggtcggc gtcatacaaa cccgcaaggc
    21661 gttgcaggca gcgggcatga cttttcgcgt cagcgatatt ccccgtgatt tacgcggcgg
    21721 ctgcgggtta tgcatttggc tgacctgtcc gcctggcgag gaaattcaat gggtgatccc
    21781 tgggcatacg gaatcagttt attgtcagca ggatggtggc tggcgctgca ttgcacatta
    21841 cgggatttct ccgcgttaag gattcattaa gatcgctacg tatacttctc gatatcttat
    21901 tgatttgcct taaagggatt accgtgacaa aaagtatttc gttaagtaaa cgcatcttcg
    21961 ttattgtgat tctttttgtc attgtggcag tgtgtacttt cttcgtgcaa tcttgcgccc
    22021 gtaaaagcaa tcatgcagca tcatttcaga attaccacgc caccattgac ggcaaagaaa
    22081 ttgcaggcat aacgaataat atctcttcgc tgacctggtc ggcacaaagc aatactcttt
    22141 ttagcactat caacaaacct gccgccattg ttgaaatgac caccaatggc gatttgatcc
    22201 gcacaattcc tctggatttt gttaaggatc tcgagacgat cgaatacatt ggcgataatc
    22261 agtttgtcat tagcgatgag cgcgattatg ccatttatgt gatttcactg accccaaact
    22321 cggaagtaaa aatcctcaaa aaaataaaaa tcccgctgca ggaatcccca accaattgcg
    22381 gttttgaagg attggcctat tctcgccagg atcacacttt ctggtttttt aaagagaaaa
    22441 acccgattga ggtctacaaa gttaacgggc tgttaagtag caatgaattg cacatcagca
    22501 aagacaaggc actacaacgg caatttactt tagatgatgt gtccggcgca gaatttaacc
    22561 aacaaaaaaa tacgcttctg gtgctgtctc atgaatctcg cgcactccag gaagtaacgc
    22621 tggttggcga ggttatcggt gaaatgtcac taaccaaagg cagtcgaggt ttgtcgcata
    22681 acatcaaaca ggccgaaggg gttgcgatgg atgcctctgg taatatttat atcgttagcg
    22741 aaccaaatcg tttttatcgc tttacccctc aatcatcgca ttaacaggct ggcatgcttc
    22801 acctttttgt tattaacttg ttaacaacaa aaatgacaaa caaggttaaa catgccttcg
    22861 tccacgcatc ccgtagaacg cttttctttc agcaccgcgc ttttcgggat gctggttctg
    22921 accttaggta tgggtttagg ccgcttcctt tatacgccta tgttgcccgt catgatggcg
    22981 gaaggatcgt tttcatttag ccagctctcg tggattgcca gcggcaacta tgcggggtat
    23041 ctggctggca gtctgctatt ttcgtttggc gcatttcacc agccatcgcg cctgcgccca
    23101 tttctgttgg cttctgccct ggcgagcggg ttgttgatcc tcgcaatggc atggttgccg
    23161 ccatttattc tggtgttatt gattcgcgtc ctggcgggtg tcgccagcgc cggtatgctg
    23221 atttttggtt cgacgctgat tatgcagcac acgcgccatc cttttgtgct ggcagctttg
    23281 ttttctggcg ttggcattgg catcgcactg ggcaatgaat atgttctggc aggcctgcat
    23341 tttgacctct cctcgcaaac gttatggcaa ggcgccgggg cgctttctgg catgatgctg
    23401 attgcactta cgcttttaat gccctcgaaa aaacacgcca tcacaccaat gccattggca
    23461 aaaacggagc aacagataat gagctggtgg ttactggcta ttctgtacgg cctggcggga
    23521 tttggttata tcatcgtcgc gacttacctg ccgctcatgg cgaaagatgc aggctcacca
    23581 ttgttaaccg cccatctctg gacgttagtc ggcttatcaa ttgtgcctgg ttgctttggc
    23641 tggctatggg cagcaaaacg ttggggggct ttgccctgcc tgacggcgaa tttgctggtg
    23701 caggctatct gtgtgctgct tactctcgcc agcgactcgc ctctcttgct tatcatcagc
    23761 agtcttggtt ttggcggtac ctttatgggc acgacatctc tggtgatgac tatcgcccgc
    23821 caactgagcg taccgggaaa tctcaatctt ttaggctttg tgacgctcat ttacggcatt
    23881 gggcaaatcc tcggcccggc gctgaccagt atgctcggca atggaacatc ggcgcttgcc
    23941 agcgccactc tctgcggcgc ggcggcgttg tttattgctg cgcttatcag cacagtgcaa
    24001 ttatttaagc tacaagtggt cacttcatga gggaagtttc ccttaccact caagaaactc
    24061 aaccgcaacc gttgatgtca gccagacccc gttttccgct tgccagaaag ggaggcctcg
    24121 tttcgccatt tcctgtgctt tgacggttaa aataaccgga gatccgtgtc ttgctcccac
    24181 tttgcgtgcc gttgcttcat cagcagagag atgtacgtag tgacgctcac ctgcaatcag
    24241 cccctgtttt tttatctcgt caaggaaacg gcttgccgta ccgtggtaga gaaactgtgg
    24301 cggtgttttt tcggcaaagg aaatagcgac ctgcgaagtc gaatgcccct gaaccgcgcg
    24361 gatgcaccta ccatcactgg aataactaaa acgcttttta tcgctggtgg caaccacagt
    24421 atccagcaat gcacgggtta accgcttgcc cgctttttgg gcacaaagta tgagcttatc
    24481 gatatccgcc catccttcac ggtccagtac gataccaatc gcctccggtt tgtgtcggag
    24541 gacaaaactt aaaaacttgc tcgtatccgc cagctctttt tcgttgtact ttgccatcgc
    24601 gttttcgtcc ctggattatc tgttttttat ttactatgtc attgtcactg ggtttatgaa
    24661 ttttctgcaa ctcaatcctg cgtagaacct tttccacaca gcgtaatttt accgctatgc
    24721 aaaaaacgca tagtcagaaa catacttgca ttttccccat acccacatgg ctgataaggt
    24781 ttttcagctc caggaatttt cctggctcaa gcaacacaca aacacaaaaa aacaatgggg
    24841 attgttatga cacaacaagg ggatgctgta gccggggagc tggcgacaga aaaagtgggc
    24901 attaaagggt atctggcttt ttttctcacc ataatctttt tttccggtgt tttttccggg
    24961 acagacagtt ggtggcgcgt ttttgatttt tccgttctga acggatcgtt tgggcaactg
    25021 cctggtgcga acggagccac cacctcgttt cgcggcgcgg gtggcgcggg ggctaaagat
    25081 ggctttctct ttgcgctgga gctggcaccg tcagtcattc tctctctggg gattatttca
    25141 attaccgatg gtcttggcgg attacgcgcc gcgcagcagt taatgacgcc agtgctaaaa
    25201 ccgttgttgg gcattccagg gatctgctcg ctggcgctga tcgctaacct gcaaaacacc
    25261 gatgccgccg caggtatgac caaagagctg gcgcaggaag gtgaaattac cgaacgcgat
    25321 aaagtgattt tcgccgcata tcaaaccagc ggtagcgcca ttatcaccaa ctacttctct
    25381 tccggcgtcg cggtgtttgc ctttctgggc acgtccgtaa tcgtaccttt agcagtcatc
    25441 ctggtgttta aatttgtcgg agccaacatt ttgcgcgtct ggcttaactt cgaagaacgt
    25501 cgcaacccaa cgcaaggagc gcaagcatga caactcaggt ccgcaaaaac gtcatggata
    25561 tgtttatcga tggtgcgcgc cgtggcttta ctatcgccac gaccaacctg ctgccaaacg
    25621 tggtgatggc gtttgtcatc attcaggcgc tgaaaattac cggcctgctc gactgggtgg
    25681 ggcatatctg tgaacccgta atggcgctgt ggggattacc cggtgaagcg gcaaccgtcc
    25741 ttttagccgc attaatgagt atgggtggcg cagtcggtgt tgccgccagt ctggcaaccg
    25801 ctggcgcatt aaccggtcac gatgtcaccg ttttactgcc cgcgatgtat ttaatgggca
    25861 acccggtgca aaatgtcggt cgctgcctgg ggacagccga agtgaatgcc aaatattacc
    25921 cgcatatcat tacggtctgc gtgattaacg cattgctgtc gatctgggtt atgcagctta
    25981 ttgtttaaca aggagttacc atgattgatt ataccgcagc cggttttacc ctgctgcagg
    26041 gagcgcattt gtatgcgccg gaagatcggg gaatttgcga tgtcctcgtc gctaacggca
    26101 aaattatcgc cgttgccagt aatatccctt ctgacattgt accggactgc acggttgtcg
    26161 atctcagcgg gcagatcctc tgcccaggtt ttattgatca acacgtccat ttgattggcg
    26221 gtggcggcga agcaggtccc acgacgcgca cgccggaagt ggcgttaagt cgtctgacgg
    26281 aagcaggcgt cacgtcagtg gttggtctgc tgggcaccga ctctatctct cgccacccgg
    26341 aatccctgct cgccaagacc cgtgcgctca atgaagaagg catcagcgcc tggatgctga
    26401 ccggcgctta tcatgtccct tcccgcacta ttacgggttc cgtggaaaaa gacgtggcga
    26461 ttatcgatcg cgtgattggc gtgaaatgcg ccatctctga tcaccgttct gccgcaccgg
    26521 acgtttatca cctggctaat atggcggcgg aatcccgcgt tggcggtttg ctcggcggta
    26581 aacctggcgt caccgtgttc cacatgggcg acagcaaaaa ggcgttacag cctgtctatg
    26641 acctgctgga aaactgcgat gtgccgatta gcaagttgct gccgacccac gttaaccgca
    26701 acgttccgtt gtttgagcag gcgctggagt tcgcgcgcaa aggcggcacc atcgatatca
    26761 ccagcagcat tgacgaacca gtcgcccctg ccgaaggtat tgcccgcgcc gttcaggcgg
    26821 gtattccgct ggcacgcgtc accctcagct ccgacggcaa cggtagccag ccgttcttcg
    26881 atgacgaagg gaatttaacc catatcggtg ttgccggttt tgaaacgttg ctggaaaccg
    26941 tgcaggtgct ggtcaaagat tatgatttca gtatcagcga tgccctgcgc ccgctcacca
    27001 gtagcgtagc cggtttcctt aacctgagcg ggaaaggcga aattctgcca ggcaatgacg
    27061 cagacttact ggtcatgacg ccagaattac gcattgagca ggtatacgct cgcggcaagc
    27121 tgatggtcaa agacggcaaa gcctgcgtga aaggaacgtt tgaaacggca taaacggttg
    27181 tcagccgccc gaatggacgt agaatgcccg caagggcggc tgacagagta aaacgtaatg
    27241 gatgactgtg gtgcggtttt gcataatatt gaaactaaat ggctttatga ttttctgacc
    27301 ctggaaaaat gccgcaattt ttcccaggca gcagtcagtc gcaacgtctc gcaaccggca
    27361 ttcagccgcc gcatccgtgc gctggaacag gcgattggcg ttgaattgtt taaccgccag
    27421 gtgacgccgc tgcaactctc ggaacaaggt aaaatattcc attcgcagat ccgccatctg
    27481 ttgcaacagt tagagagcaa ccttgcagaa ctccgtggtg gcagcgatta cgcgcaacgc
    27541 aaaattaaaa tagccgctgc acactctctt tccctcgggc tgttaccgtc cattatcagc
    27601 cagatgccgc cgctctttac ctgggcaatt gaagctattg atgtcgatga agcggtcgat
    27661 aaactgcgtg aagggcaaag tgactgtatt ttttcctttc acgacgaaga tttgctggaa
    27721 gcgccgtttg accacattcg cttatttgaa tctcaattgt tccctgtctg cgccagtgac
    27781 gaacacggag aagcactttt taacctcgcg cagccacact ttccgttact gaattacagc
    27841 cgcaactcct acatggggcg attgattaat cgcaccctga cgcgccacag tgagttaagt
    27901 ttcagcacct tttttgtctc ttcgatgagc gagcttttaa agcaggttgc cctcgacggc
    27961 tgtgggattg cctggctgcc ggagtacgcc atccaacaag aaattcgcag cgggcagctc
    28021 gttgtactga atcgggacga actggtgatc ccgattcagg cctatgcata ccgaatgaat
    28081 acccgcatga atccagttgc cgaacgcttc tggcgtgaac tgcgcgagct ggagattgta
    28141 cttagctgac attctcgagc gtcgcactac gccaaccgcc attatcccag accagatcaa
    28201 acgttagcgg ttcatcgtcg tggtacagca ttatctcaag gctgatcgcc agcgtgtggc
    28261 tgtacgcttc tttcagattg acctgcaacg ccgcaatccg tggctcatga aataacagtg
    28321 cagaacgcag ctgacggcaa taccagctct ggagatgttt gtcgctctgg ttatgcactt
    28381 gccactcggg catgcctgaa acaagtaatg acggtaatgc cgaatgagga gaattaaaaa
    28441 gagtgaggag atcgctacat attgatttac gcagcgatga cgttttattg cctgaaattt
    28501 caggtaaaac agcctgacgt gattggcgca tcattttgcg cactcctgac gtttagcaat
    28561 atatgcggct tgccctgaac accgggcacc actgtattga tgctcatttt ccatagccat
    28621 tcccttttgt tcattacatc catgtcagtc tgagacaggc ctgaatcctg tcagcaaagt
    28681 ataaaacctt acttgcctat gaatatctac tgacttttca ggaaaaaagt catgatttga
    28741 taaaaatcat caaactca
//
LOCUS       DACBVI010000047        28152 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.047, whole genome shotgun sequence.
ACCESSION   DACBVI010000047 DACBVI010000000
VERSION     DACBVI010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.047"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..376
                     /gene="cmtB"
                     /locus_tag="IU325_10030"
     CDS             <1..376
                     /gene="cmtB"
                     /locus_tag="IU325_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HAP5809360.1"
                     /translation="AIDFSMASLLDKNYISENYIQAIKDSTINNGSYYTLAPGVAMPH
                     ARPECGALKTGMSLTLLKQGVYFPGNDEPIKLLIGLSAADADSHIGAIQALSELLCEE
                     EILEQLLTASSEKQLADIISRG"
     gene            404..1792
                     /locus_tag="IU325_10035"
     CDS             404..1792
                     /locus_tag="IU325_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP5809361.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYIDKSLEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETENEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKN"
     gene            1807..3084
                     /locus_tag="IU325_10040"
     CDS             1807..3084
                     /locus_tag="IU325_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAP5809362.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLAGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     EKLLPVELAASKGIELVYVNTKGMSDPVQTLRALTDDAGFDDVFVYAAVPSVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3081..4046
                     /locus_tag="IU325_10045"
     CDS             3081..4046
                     /locus_tag="IU325_10045"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAP5809363.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSMPLADNLRNVAKALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKEMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4068..4577
                     /gene="fumE"
                     /locus_tag="IU325_10050"
     CDS             4068..4577
                     /gene="fumE"
                     /locus_tag="IU325_10050"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HAP5809364.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDITWDFISNVNSIIRNASLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4574..5287
                     /locus_tag="IU325_10055"
     CDS             4574..5287
                     /locus_tag="IU325_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HAP5809365.1"
                     /translation="MKIELTVNGLKVQAQYSDDEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFLEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WQELASFCDFSIFIHAPAQILRERLISRKIAGGLSRQEAEAFYARTDGPNVERVLMNS
                     CQANLIVEMTEEGRYHFKS"
     gene            5572..6591
                     /locus_tag="IU325_10060"
     CDS             5572..6591
                     /locus_tag="IU325_10060"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HAP5809366.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSSIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            6641..7804
                     /locus_tag="IU325_10065"
     CDS             6641..7804
                     /locus_tag="IU325_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP5809367.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            8019..9098
                     /gene="fbaA"
                     /locus_tag="IU325_10070"
     CDS             8019..9098
                     /gene="fbaA"
                     /locus_tag="IU325_10070"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HAP5809368.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            9289..10149
                     /gene="mscS"
                     /locus_tag="IU325_10075"
     CDS             9289..10149
                     /gene="mscS"
                     /locus_tag="IU325_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HAP5809369.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            10288..10923
                     /gene="argO"
                     /locus_tag="IU325_10080"
     CDS             10288..10923
                     /gene="argO"
                     /locus_tag="IU325_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HAP5809370.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            11016..11756
                     /locus_tag="IU325_10085"
     CDS             11016..11756
                     /locus_tag="IU325_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HAP5809371.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            11924..12820
                     /locus_tag="IU325_10090"
     CDS             11924..12820
                     /locus_tag="IU325_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP5809372.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTSH"
     gene            complement(12817..14295)
                     /gene="scpC"
                     /locus_tag="IU325_10095"
     CDS             complement(12817..14295)
                     /gene="scpC"
                     /locus_tag="IU325_10095"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HAP5809373.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGEIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(14319..15104)
                     /gene="scpB"
                     /locus_tag="IU325_10100"
     CDS             complement(14319..15104)
                     /gene="scpB"
                     /locus_tag="IU325_10100"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HAP5809374.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(15115..16110)
                     /gene="meaB"
                     /locus_tag="IU325_10105"
     CDS             complement(15115..16110)
                     /gene="meaB"
                     /locus_tag="IU325_10105"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HAP5809375.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGINEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(16103..18247)
                     /gene="scpA"
                     /locus_tag="IU325_10110"
     CDS             complement(16103..18247)
                     /gene="scpA"
                     /locus_tag="IU325_10110"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HAP5809376.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYVESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTELFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGRKDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(18451..19344)
                     /locus_tag="IU325_10115"
     CDS             complement(18451..19344)
                     /locus_tag="IU325_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgP/LysG family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP5809377.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            19486..19716
                     /locus_tag="IU325_10120"
     CDS             19486..19716
                     /locus_tag="IU325_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809378.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLANGELVRLFAPWSGIP
                     RLLYALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            19772..20431
                     /gene="rpiA"
                     /locus_tag="IU325_10125"
     CDS             19772..20431
                     /gene="rpiA"
                     /locus_tag="IU325_10125"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP5809379.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            20688..21920
                     /locus_tag="IU325_10130"
     CDS             20688..21920
                     /locus_tag="IU325_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP5809380.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            22149..22208
                     /gene="ibsC"
                     /locus_tag="IU325_10135"
     CDS             22149..22208
                     /gene="ibsC"
                     /locus_tag="IU325_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HAP5809381.1"
                     /translation="MMRLVIILIVLLLISFPAY"
     gene            complement(22309..22911)
                     /locus_tag="IU325_10140"
     CDS             complement(22309..22911)
                     /locus_tag="IU325_10140"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP5809382.1"
                     /translation="MTQLPELPQTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVQEWDIPLPAVVTPSKVWEW"
     gene            complement(22932..23115)
                     /gene="ssrS"
                     /locus_tag="IU325_10145"
     ncRNA           complement(22932..23115)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IU325_10145"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23157..23486)
                     /gene="zapA"
                     /locus_tag="IU325_10150"
     CDS             complement(23157..23486)
                     /gene="zapA"
                     /locus_tag="IU325_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP5809383.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAANMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            23654..24232
                     /gene="ygfB"
                     /locus_tag="IU325_10155"
     CDS             23654..24232
                     /gene="ygfB"
                     /locus_tag="IU325_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HAP5809384.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            24258..25583
                     /gene="pepP"
                     /locus_tag="IU325_10160"
     CDS             24258..25583
                     /gene="pepP"
                     /locus_tag="IU325_10160"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HAP5809385.1"
                     /translation="MSDISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPIVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSMQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            25580..26758
                     /gene="ubiH"
                     /locus_tag="IU325_10165"
                     /gene_synonym="visB"
     CDS             25580..26758
                     /gene="ubiH"
                     /locus_tag="IU325_10165"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HAP5809386.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITSVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGCVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQRDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            26781..27983
                     /gene="ubiI"
                     /locus_tag="IU325_10170"
                     /gene_synonym="visC"
     CDS             26781..27983
                     /gene="ubiI"
                     /locus_tag="IU325_10170"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HAP5809387.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAANA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATISTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESERLVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELVAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(28057..28140)
                     /locus_tag="IU325_10175"
     CDS             complement(28057..28140)
                     /locus_tag="IU325_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809388.1"
                     /translation="MSKNTKSKIRGIRKYNAKTEVKLVYFK"
BASE COUNT         6701 a         6921 c         7574 g         6956 t
ORIGIN      
        1 agctatcgat ttctcgatgg catcattgct ggataaaaac tatatcagcg agaattacat
       61 tcaggcaatt aaagattcca ccattaacaa tggctcttat tatactctcg caccaggtgt
      121 ggcaatgcct catgcgcgac cggaatgtgg ggcgcttaaa accgggatgt ctttaacgtt
      181 acttaaacaa ggtgtttatt ttccggggaa tgacgaaccg ataaaattac tcatcggcct
      241 ttctgctgcc gatgccgatt cgcacattgg cgctattcag gcgttaagtg aattactgtg
      301 cgaagaagaa atactcgaac aactcttaac agcatcatca gaaaaacaat tagcggacat
      361 tatcagccgc ggataatgac tctctcatct acaggataat attatggaaa acaagtctgc
      421 tcgtgcaaag gtccaggctt ttgggggctt tttgactgca atggtcatcc ccaatatcgg
      481 cgctttcata gcctggggtt ttataacggc gttatttatt cccaccggct ggctgccgaa
      541 tgagcatttc gccaaaattg tcggtccgat gattacctat ttattgcccg tgatgattgg
      601 ttctaccggt ggtcatctgg tcggcggtaa acgcggcgcg gtcatgggcg gaattggtac
      661 gattggtgtg atcgtcggcg ctgaaatccc gatgttcctt ggctcgatga ttatggggcc
      721 gctcggtggt ctggtgatta aatatatcga caagtcgctg gaaaaacgca tacctgctgg
      781 ttttgagatg gttatcaata acttctcatt aggtatcgcg gggatgctcc tttgtctgct
      841 gggttttgaa gtgatcggcc ctgcggtgtt aattgccaat actttcgtca aagagtgtat
      901 tgaggcgctg gtacatgcgg gttatctgcc tttgctgtca gtcatcaatg aaccggcgaa
      961 agtgcttttc cttaataatg cgatcgatca gggcgtctat tatccgctgg gaatgcaaca
     1021 ggcatcagtt aacggcaaat ccatcttctt tatggtggca tccaatccag ggcctggctt
     1081 aggactgttg ctggcattca ctctgtttgg taaagggatg agtaagcgct ccgcacctgg
     1141 tgcgatgatt attcacttcc tcggcggtat ccacgaactg tatttcccgt atgtgctgat
     1201 gaagccactg accattattg ccatgattgc gggcggtatg tctggcacct ggatgtttaa
     1261 cttactggac ggtggtctgg tggctggccc aagtccgggg tctatctttg cttacctggc
     1321 gctgacgccg aaaggctcat tcctggcgac aattgctggt gttacggtag gtaccctggt
     1381 gtcctttgct atcacttcgc tgatactgaa gatggaaaaa acggtggaaa cggagaacga
     1441 agatgagttt gctcagtcag ccaatgcggt taaggcgatg aaacaagagg gtgcattctc
     1501 gttaagccgg gttaagcgta tcgcctttgt ttgcgatgcg gggatgggct ccagcgcgat
     1561 gggcgcaacc accttccgta aacgtctgga aaaagcgggg ctggcaattg aagtaaaaca
     1621 ttacgcgata gaaaacgtgc ctgcggatgc ggatatcgtt gttactcatg ccagtctgga
     1681 agggcgcgtg aaacgtgtga cggataaacc gctgatactg attaataact atattggcga
     1741 tccaaaactc gacactttat ttaatcaatt aaccgccgaa cataaaaact gattgccgag
     1801 gtaaaaatga aaaccaaagt tgctgctatt tatggcaagc gggatgtccg tctgcgcgaa
     1861 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtgatttc tgacagcgtc
     1921 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
     1981 ttagaaaatc atccggtcat taccgggcat gaatgtgccg gggttattgt tgaagtgggt
     2041 aaaaatctcg ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
     2101 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggtaa cgccacctat
     2161 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
     2221 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggcgctta tcatgccaat
     2281 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg ttaaacctgg cggcaatatt
     2341 gcgctgctgg catgtgctgg tccgatgggc attggcgcta tcgattacgc cattaacggc
     2401 ggcatacaac cgtcgcgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggta
     2461 gagaagctgc tgccggtgga actggcggcc agtaaaggca ttgagctggt gtatgtgaat
     2521 accaaaggga tgagcgatcc ggtccagacg ctgcgggcgc tgacggatga tgccggattt
     2581 gatgacgtat ttgtctatgc agcggtgcct tcagtcgtcg agatggctga tgaattactg
     2641 gcggaagatg gctgcctgaa cttctttgcc gggccgacgg ataaaaactt caaagtgccg
     2701 tttaatttct acaacgtcca ttacaacagc acgcatgtcg tcggtacgtc cggcggttca
     2761 acggacgaca tgaaagaggc gattgcgctt agcgccacag ggcagttgca gccgtcgttt
     2821 atggtgaccc atatcggtgg gctggatgcg gtgcctgata ccgtgctcaa tctgccggat
     2881 atccccggcg gtaaaaaact catttataac ggcgtgacca tgccgctgac tgccattgcc
     2941 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agctggcgcg gctggttgag
     3001 gaaacgcacg gcatctggaa tgagcaggcc gagaaatatc tgctggcgca atttggcgtt
     3061 gatatcgggg aggccgcaca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     3121 aggcagcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     3181 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtggg cgagtggtta
     3241 tcggcgaggg agagattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     3301 gcgatgggcc agaagtcgat atcgcggttg acccgattga aggtacgcgg atggtggcga
     3361 tggggcagag caatgcgctg gcggtcatgg ctttcgcccc tcgagatagc ctgttacacg
     3421 cgcccgatat gtatatgaaa aaactggttg ttaatcggct ggcggcgggc gcgattgatc
     3481 tgtccatgcc tctggcggac aacttgcgca acgtggcgaa agcgttaggc aaaccgctgg
     3541 acaagctgcg tatggttacg ctggataaac cgcgcctgag tgccgcaatt gaggaagcaa
     3601 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     3661 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     3721 tgatttctgc ctgtgccgtt aaagcattag gcggggatat gcaggccgag ctgattgact
     3781 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     3841 gttgcaagga aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     3901 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagctagtg aacggtatcc
     3961 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     4021 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg gcgacgctga
     4081 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     4141 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     4201 atgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     4261 acgatattga tgttgcactg cgtttgattt acgcgctggg gaaaatggat aaatggttgt
     4321 acgccgatat cacgcatttt tcccagtttt ggcattacct gaacgaacag gatgaaacgc
     4381 ccggatttgc cgatgatata acctgggatt ttatcagcaa cgtcaatagc ataatccgca
     4441 atgcgagtct ctatgacgcg ttgaaagcga tgaaattcgc cgatttcgcc gtctggtcag
     4501 aggcgcgttt tagcgggatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     4561 aggaattaac gccgtgaaaa tcgaactgac ggtgaatgga ctaaaggtac aggcgcagta
     4621 ctctgatgat gaaattgaaa atgttcataa accgctactg cgtatgctgg cggccttaca
     4681 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     4741 atctacccta accaccttct tggaatatct cgcgcaacaa gacccggaac tacccgctat
     4801 tcagacgctg ccgatggatg gttttcatca ttacaatagc tggctggatg cgcatcaatt
     4861 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     4921 ccaggtcgtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     4981 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggctattact
     5041 ggatgatgaa aagtggcagg agttggcatc tttctgcgat ttttctattt ttattcatgc
     5101 acctgctcaa attctgcggg agcgcttgat tagtcgtaaa attgcgggag gattatcgcg
     5161 gcaagaagcc gaagcttttt atgcccgaac cgatggcccc aatgtcgagc gtgtgctgat
     5221 gaatagttgt caggccaatc tgatagtaga gatgaccgaa gaaggcagat atcacttcaa
     5281 aagctgacaa tttattccag ttattgcagg ggtattatta cgccagcctg gaataaagcc
     5341 ccctacaaaa ggggataaaa gtgtgatgta agtcagataa atgtcttctt tgactggaca
     5401 atcattcctt ttattccacg tttcgcttat cctagctgaa gcgtttcagt cgattaaatg
     5461 ttcgacaatt aaccaatcag tcgcagtttg cgacaggtaa ggtttccccg gacgatttgc
     5521 tggattactc tgtctggcaa atttgctcag ggaaaacctt gcaggagatc tatgaccgta
     5581 cgcgtagcga taaatggctt cggtcgcatc gggcgtaatg tggttcgtgc cttgtatgaa
     5641 tccggacgcc gggcggaaat taccgtggtg gcaatcaacg aactggcgga tgctgcgggc
     5701 atggcgcatt tgttgaaata tgacaccagc catggccgtt ttgcatggga agtacgacag
     5761 gaacgcgatc aactttttgt tggtgatgac gccatccgcg tattgcatga acgttcactg
     5821 caatcgctcc cctggcgtga acttggcgtt gatgtagtcc tcgactgcac cggcgtatat
     5881 ggctcccgcg agcatggcga agcgcatatt gccgccgggg ccaaaaaagt gctcttttca
     5941 catcctggca gtaacgatct cgacgcgacc gttgtttacg gcgtcaatca ggatcaactt
     6001 cgtgcggaac accgcatcgt ttctaacgct tcctgtacca cgaattgcat aattcccgtc
     6061 atcaaattgt tagatgatgc gtacggtatt gagtccggca ctgtgaccac aattcactcc
     6121 gccatgcacg atcaacaggt tattgatgca taccatcctg acctgcgtcg cacccgggca
     6181 gccagccagt cgagcattcc ggtcgatact aaactggccg ccggtatcac acgatttttt
     6241 ccgcaattta acgatcgctt tgaagcgatt gcggtacgtg tgccaaccat aaatgtgacg
     6301 gcaatcgatt taagcgtgac ggtgaagaaa cctgtaaaag ccaatgaagt caacctgttg
     6361 ctgcaaaaag cagcacaagg tgcatttcat ggtatagttg actatacgga attgccgttg
     6421 gtctctgtag attttaacca cgatccgcac agtgccattg tcgatggcac ccaaacccgg
     6481 gtcagtggcg cacacctgat caaaacgttg gtctggtgcg ataacgaatg gggctttgct
     6541 aaccgaatgc tcgacacgac gttagctatg gctactgttg ctttcaggta agacgcaagc
     6601 agcgtctgca aaacttttag aatcaacgag aggattcacc atgtctgtaa ttaagatgac
     6661 cgatctggat cttgctggga aacgtgtatt tatccgtgcg gatctgaacg taccagtaaa
     6721 agacgggaaa gtaaccagcg acgcgcgtat ccgtgcttct ctgccgacca tcgaacttgc
     6781 cctgaaacaa ggcgcaaaag tgatggtaac ttcccacctg ggtcgtccta ccgaaggcga
     6841 gtacaacgaa gaattctctc tgctgccggt tgttaactac ctgaaagaca aactgtctaa
     6901 cccggttcgt ctggttaaag attacctcga cggcgttgac gttgctgaag gtgaactggt
     6961 tgttctggaa aacgttcgct tcaacaaagg cgagaagaaa gacgacgaaa ccctgtccaa
     7021 aaaatacgct gcactgtgtg acgtgttcgt aatggacgca ttcggtactg ctcaccgcgc
     7081 gcaggcttct actcacggta tcggtaaatt cgctgacgtt gcgtgcgcag gtccgctgct
     7141 ggcagctgaa ctggacgcgc tgggtaaagc actgaaagaa cctgctcgcc cgatggtggc
     7201 tatcgttggt ggttctaaag tttctaccaa actgaccgtt ctggattccc tgtctaaaat
     7261 cgctgaccag ctgattgttg gtggtggtat cgctaacacc tttatcgcgg cacaaggcca
     7321 cgatgtgggt aaatccctgt acgaagctga tctggttgac gaagcaaaac gtctgctgac
     7381 cacctgcaac atcccggttc cgtctgatgt tcgcgtagca actgagttct ctgaaactgc
     7441 accggctacc ctgaaatctg ttaacgatgt gaaagctgac gagcagatcc tggatatcgg
     7501 tgatgcttcc gctcaggaac tggctgaaat cctgaagaat gcgaaaacca ttctgtggaa
     7561 cggtccggtt ggcgtgttcg aattcccgaa cttccgcaaa ggtactgaaa tcgtggctaa
     7621 cgctatcgca gacagcgaag cgttctccat cgctggcggc ggcgacactc tggcagcaat
     7681 cgacctgttc ggcattgctg acaaaatctc ctacatctcc actggcggcg gcgcattcct
     7741 cgaattcgtg gaaggtaaag tactgcctgc agtagcgatg ctcgaagagc gcgctaagaa
     7801 gtaaaaaatc acagggcagg gaaacctgcc cttgtttcag cgcgctttta aagcacgcac
     7861 tttttcatac tctaaataat tcgagttgca ggaaggcgac aagagagtga atccccagga
     7921 gcttacataa gtaagtgact ggggtgagcg aacgcagacg cagcacatgc aacttgaagt
     7981 atgacgagta taaggcccga cgatacagga caagagacat gtctaagatt tttgatttcg
     8041 taaaacctgg cgtaatcact ggtgatgacg tacagaaagt tttccaggta gcaaaagaaa
     8101 acaacttcgc actgccagca gtaaactgcg tcggtactga ctccatcaac gccgtactgg
     8161 aaaccgctgc taaagttaaa gcgccggtta tcgttcagtt ctccaacggt ggtgcttcct
     8221 ttatcgctgg taaaggcgtg aaatctgatg ttccgcaggg cgctgctatc ctgggcgcga
     8281 tctctggtgc gcatcacgtt caccagatgg ctgaacatta tggtgttccg gttatcctgc
     8341 acaccgacca ctgcgcgaag aaactgctgc cgtggatcga cggtctgctg gacgcgggtg
     8401 agaaacactt cgctgctacc ggtaagccgc tgttctcttc tcacatgatc gacctgtctg
     8461 aagaatctct gcaagagaac atcgaaatct gctctaaata cctggagcgc atgtccaaaa
     8521 tcggcatgac tctggaaatc gaactgggtt gcaccggtgg tgaagaagac ggcgtggaca
     8581 acagccacat ggacgcttct gcactgtaca cccagccgga agacgttgat tacgcataca
     8641 ccgagctgag caaaatcagc ccacgtttca ccatcgcagc gtccttcggt aacgtacacg
     8701 gtgtttacaa gccgggtaac gtggttctga ctccgaccat cctgcgtgat tctcaggaat
     8761 atgtttctaa gaaacacaac ctgccgcaca acagcctgaa cttcgtattc cacggtggtt
     8821 ccggttctac tgctcaggaa atcaaagact ccgtaagcta cggcgtagtg aaaatgaaca
     8881 tcgataccga tacccaatgg gcaacctggg aaggcgttct gaactactat aaagcgaacg
     8941 aagcttacct gcagggtcag ctgggtaacc cgaaaggcga agatcagccg aacaagaaat
     9001 actacgatcc gcgcgtatgg ctgcgtgccg gtcagacttc gatgatcgct cgtctggaaa
     9061 aagcattcca ggaactgaac gcgatcgacg ttctgtaagt cattcttttc tgcttatctc
     9121 aaggcccgct ctgcgggtct ttttttcgcc aaaagcaagg tgatacagat gagaaatctg
     9181 tgatctattt ggcaaaatta tgctttattg tttacccttg tcagactgcc cgtcataagg
     9241 cggcggagtg tatttctcca ttttgagtca gttgaaaagg aatattgaat ggaagatttg
     9301 aatgttgtcg atagcataaa cggcgcggga agctggctgg tggctaacca ggcgctgctg
     9361 ctaagttatg cagtaaacat cgtggcggca ctcgcgatca tcatcgttgg tttgattatc
     9421 gcgcggatga tttccaacgc ggtgaatcgc ctgatgatct cccgtaaaat cgatgccact
     9481 gttgctgatt ttctctctgc attagtccgt tacggtatta tcgcctttac gctaatcgct
     9541 gcactggggc gcgtgggtgt acaaaccgcg tccgtcattg ctgtactcgg tgccgcaggc
     9601 ttagctgttg gtctggcttt gcaggggtca ctttctaacc tggccgctgg cgtgttactt
     9661 gtcatgttcc gcccgttccg tgccggagaa tatgttgacc tcggcggcgt agccggtact
     9721 gtgctgagtg tgcagatttt ctccaccacc atgcgtactg cagacggtaa aattatcgtt
     9781 attccgaacg gtaaaattat tgccggaaat attattaact tctctcgcga gccagttcgc
     9841 cgtaacgaat ttattattgg cgtggcgtat gattccgata tcgatcaggt taagcagatc
     9901 ctgaccaata ttatccagtc tgaagatcgc attttgaaag atcgcgaaat gactgtgcgc
     9961 ctgaatgaac ttggcgcatc gtcgattaat ttcgtggtcc gcgtctggag caacagcggc
    10021 gatctgcaaa acgtgtactg ggatgtgctg gagcgtatta aacgtgaatt tgatgccgcc
    10081 ggtatcagct tcccgtaccc gcaaatggat gtgaacttta agcgggtgaa agaagacaaa
    10141 gctgcgtaat caacgctgag gccagataat actctctggc ctctctctta ttagtttttc
    10201 tgattgccaa ttaatattat caatttccgc taataacaat cccgcgatat agtctctgca
    10261 tcagatactt aattcggaat atccaacgtg ttttcttatt actttcaagg tcttgctctt
    10321 ggggcagcta tgattctgcc gctcggccca caaaatgctt ttgtgatgaa tcagggcatt
    10381 cgtcgtcagt accacattat gattgcctta ctttgcgcta tcagcgattt ggtcctgatt
    10441 tgcgcgggga tttttggtgg cagcgcgtta ttgatgcagt cgccgtggtt gctggcgctg
    10501 gtcacctggg gcggcgtagc cttcttgctg tggtatggtt ttggcgcttt taaaacagcg
    10561 atgagcagta atattgagtt agccagtgcc gaagtcctga agcaaggcag atggaaaatt
    10621 atcgccacca tgctggcagt gacctggctg aatccgcatg tttacctgga tacttttgtt
    10681 gtactgggca gccttggcgg gcaacttgat gtggaaccaa aacgttggtt tgcgctcggg
    10741 acaattagcg cctctttcct gtggttcttt ggtctggcta ttctcgcagc ctggctggca
    10801 ccgcgcctgc gcacggcaaa atcacaacgc attatcaatc tggttgtggg atgtgttatg
    10861 tggtttattg ccttgcagct ggcgagagac ggtattgctc atgcacaagc cttgttcagt
    10921 taggcgctat ctgatggaaa aataaaacag aggcgctaag cttgcctcca gaggtcctga
    10981 attttaggca agtcaattaa cacggagaga ctaacgtgaa gttcaaagtt atcgccctgg
    11041 cggcattaat gggtattagc gggatggcag cgcaggctaa cgaattgccg gatggaccgc
    11101 atattgtcac ctccggtacg gcaagcgtgg atgcggtgcc agacattgcc actcttgcga
    11161 ttgaagtcaa cgtggccgcg aaggatgccg ctactgccaa gaaacaggcg gatgagcgcg
    11221 tcgcacaata catttccttc cttgaactca atcagatcgc gaaaaaagat atcagctcag
    11281 cgaacttacg cacccagcca gattatgatt atcaggatgg taaaagtatc ctgaaaggct
    11341 accgtgctgt gagaacggtg gaagtcacgc tccgtcagtt agacaaactg aattccttgc
    11401 tggatggcgc gctgaaggcg ggtcttaacg aaattcgttc tgtgtcgctg ggcgtggcgc
    11461 agccggatgc ctataaagac aaagcgcgta aggcagcgat tgataacgcg attcatcagg
    11521 cgcaggaact ggcgaacggc tttcatcgta aactggggcc ggtatatagc gtgcgctacc
    11581 atgtttccaa ctatcagccc agcccaatgg tgcggatgat gaaagccgat gccgcgccgg
    11641 tgtccgccca ggaaacttac gagcaggccg ctattcagtt tgatgatcag gtcgatgtgg
    11701 tcttccagtt agaacctgtg gatcaacaac ccgctaaaac acctgcagca caataatatt
    11761 tatagcccga cgtattcgtc gggctaatgc ctttacccga tattgcctcc tttttctgta
    11821 attttctgta aaaccatgac gtaatcacct atctttgctc gcggtttcat caaggatagg
    11881 ctgactgaaa cggttaagcg gacaaggatg cgggggagcg gggatggaca tttttatttc
    11941 gaaaaagatg cgcaatttca ttttattagc gcaaaccaat aatattgccc gggcggcaga
    12001 aaaaatccat atgacggctt cgccatttgg taaaagcatt gccgcgctgg aagaacaaat
    12061 tggctatacg ctatttaccc gcaaagataa taatatcagc ctcaataaag ccggacagga
    12121 actgtatcaa aaactgttcc cggtttatca acggctttct gcgattgata atgaaatcca
    12181 taactcaggg cgtcgttcac gagaaattgt gattggcata gataatactt atccgacaat
    12241 tattttcgat cagttgatta gtctcggaga taagtatgaa ggcgtgaccg cccagccagt
    12301 tgagttcagc gaaaacggcg ttatcgataa tctttttgac cgtcagttag attttattat
    12361 ctccccacag catgtatctg cacgagttca ggaactggaa aacctgacta tcagtgaact
    12421 accaccgctg cgattgggct ttctcgtttc tcgccgttat gaagagcgac aagagcaaga
    12481 gctgcttcag gagctaccct ggctgcaaat gcgctttcag aaccgggcca acttcgaagc
    12541 aatgattgat gcgaatatgc gaccttgtgg gatcaatcca accattattt atcgtcccta
    12601 cagttttatg gccaaaatca gcgccgtgga gcgcggacat tttctgacgg tgatcccgca
    12661 ttttgcctgg cgactggtga atccggcaac gctgaaatat tttgatgcgc cgcacaggcc
    12721 gatgtatatg caggaatatc tttattcaat cagaaatcat cgttataccg ccacgatgct
    12781 tcagcatatt gctgaagatc gtgacgggac gagtcattaa cccagcatcg agccggttgc
    12841 aattaaatta cggtgtaaat cgaagacgtg gctaagatcg tggtgaatat gtccgccggg
    12901 cgcattttcc agatagcgat gcagataatc ccgatacata ggatgtgcac aattatcaat
    12961 gatggtgcgg gcgcgttgaa gcggggaaag accacgcaga tcggcgatcc cttgttcggt
    13021 gatgatcact ttgacgctgt gttcgctgtg atcaacatgg ctgcacattg gcacgacggt
    13081 tgagatcttg ccttctttag caatcgacgg ggccataaag atcgacagat acgcgttgcg
    13141 ttcaaaatca ccgctgccac cgatgccgtt catcagatcg accccggcta cgtgcgttga
    13201 gttggcatgc ccgtaaatat caaactccag gccgacgttc agagcgatga cgcccagacg
    13261 acggatgatt tccgggttat tggaaatctc ctgcggacgc aacacaatgc ggctggcaaa
    13321 gtaatccata ttgtcgtaaa tcttgcgcag ggaatcggcc gagatagtca ggctggaggc
    13381 gctggccccg ctgattttgc ccgtttccag taaatgcacc accgattcct gtagcacttc
    13441 cgaatacatc atgaacggag gaatttccgg gttttcccct agtcgcgcca tgactgcatt
    13501 attgatattg cccacgccac tttgcagcgg cagaaattcc ggcggaatac gcccatgcgc
    13561 catttcctgc aacaagaacg tgaccacgtt atcggcaatc tgctggcaca tgggattttg
    13621 cttatccagc atattaccgg cgtcaggcaa gttggtttct acgacggcga caatcttttt
    13681 cggatcgatt tgcacatagc gggtaccgac gcgatccatt gcatggaaga tcgacacgct
    13741 attgcgccgc ggtggcgcgc caggaatcac aatatctgcc agttctgcaa cgcgcggatc
    13801 gtgatagtga ttaagttcaa tgattacttt cttcgctcgc agcagccagg tcggcgcatt
    13861 accgatcccg ctggttaacc agactcgacc atccggtgcc agtgccgatg cttcaatgac
    13921 ggcaacatca atttcgccga agaaaccgta attgaccatt tgcgccactt cgctcaaatg
    13981 caggtcaacg aaactcacag cgccctgatt gatcttttta cgtaaaccag acgatgtttg
    14041 atatggcgca cgccaggaaa cagcatcggc gtcagaaagt acatcgtcag cggcggcgct
    14101 gattgacgcc cccgttagca ggcggatttg atacggcttt ttggcctcat gctgttcgtt
    14161 agctctgcgg gcaatcgcgg tgggaagggc tttcggcgaa cccgccgggg taaagccgct
    14221 aaatgccacc atgtcgttat gctggataat ttctgccgct tcatcggcgg tcatccttgt
    14281 ccactgagtt tccattttta ctccttcgtt cgtagggatt aatgaccaac gaaattaggt
    14341 ttacgttttt cgaggaaagc gttcatccct tcctggtaat cttcgctgtc ataaaccgcg
    14401 cggcgcatcc cctgaatacg ttcaaattca tcggagttca tggtgtgtgc ttcgcccagt
    14461 acacgcagct cttctttgat aacggcaatg gctaacggcg ctttctcaga gatgtggtgc
    14521 gccatttgta aggtgaaatc ttccagttct tccacttcca caacatggtt gaggatgccg
    14581 acagccagcg cgcgctgggc ggtgattggc gaagcggtaa aaatcagctc tttgacaatg
    14641 tggaagcccg cgtcgcgggt caggttgtga atgccgacca ggttatacgg gacaccgagg
    14701 tttacaggtg tcattgagaa ggttgaggta ctggcggcga taatcagatc ggaactcatg
    14761 atcatttcaa atgcgccacc ccaaacacta ccttccacca tcgaaatgat cggtttcggg
    14821 aatttttgga tcatgcgggt gatttgacgc aacggatcat cataggagag cggatcgcga
    14881 ccgccagacg gcagttcgtg aatatcgtga cctgcggaga agactttgga tccactcggc
    14941 gcgcgcaaaa tgatacagcg aatttccggc cggttgagat cgcttaacgc ctgcataaga
    15001 tcatcaataa agactttact taaggcatta agttttcggc catagttaaa ctcaatgacc
    15061 gccactttgt tgatagtgac aacgttcaca tactgataag acataaaaat tcctttaatc
    15121 aaaatattgc gtctggataa attcactgag ctgccgcagg ccggtgcgcg gtgagagcgt
    15181 attgtttttg accgctaaaa gcgtctggcg gtaatagcga tcgaaatctt cattcgcgaa
    15241 caggtgattc agtacttctt cttcggtttg tttacgcagc cattccaccg attgttgttg
    15301 ccgcacttgt tgcaaacgac cactggcagt tagcgcggtt ttgaagtcga tgatggcgtg
    15361 ccagatctca ttgattccac gtttttccag tgcgctacaa gtcagaaccc gtggctgcca
    15421 ttcgtcgtat ttacgtcgca gaatatgcag ggcactctcg tacatatgcc gggcaatggc
    15481 gacattggta tggttatcgc catcgtcttt gttgataacg atcagatctg ccacttccat
    15541 cagccccttt ttaatgccct gcagatcatc gccaccaccg gcaatttgca acgagataaa
    15601 acagtccacc atgcgggcga cttccgtttc tgactgcccg acgccaaccg tttcgacaat
    15661 cactacgtca taacccgccg cttcgcataa aagcattaat tcccgcgctc gctgactggc
    15721 accgcccaga tgaccggagg agggtaccgg gcgaataaac gccgcctcgg cacgcgcaag
    15781 gtcattcatg cgggttttat ccccgagaat gctaccgcca gtgaccgggc tgctgggatc
    15841 gaccgcaata accgcaacct ttaatccctc acgaatcaac aacatgccaa aggcttcaag
    15901 aaaggtactt ttccccgcgc cgggtgtgcc ggtaacgccc agtcgcaggg cgttaccaca
    15961 gaacggcata atggcgtcaa gcagctgagt acttagcgcc tgatgacgcg ggtgacggct
    16021 ttccaccaac gtcatggcct gggcgagcgt ggcacgctca ccctgacgta agcggcgaat
    16081 actttctgcc agcgtggctt cattaatcat gatgctggct tatcagattc agtacgtcgc
    16141 gcacgctgtc gagcataggt gtacctggac cataaatcgc cgccacgccg cgctcttgca
    16201 ggaaggcgta atcctgcggc ggaatgacgc cacccgcgac cacgcagata tctttgcgtc
    16261 cccatttttt cagcgcttcg accagttccg ggatcagcgt tttatgaccg gcagccagtg
    16321 aggatgcgcc cactacgtga acgtcatttt ctactgccag gcgggcgatc tcttcaggtg
    16381 tagagaacat cgggcttaaa tctacgtcga aaccgaggtc ggaataggcg ctggcgatca
    16441 cttttgcgcc gcgatcgtgc ccatcctgac ccattttagc gatcagaatg cgcgggcgac
    16501 gaccattgtc ggcaaggaac agctccgttt gcgcaacaat ggcatcgaac tcggaggccg
    16561 atttctcaga ctgatgatag ctttgcgcaa tcacgccggt aacacactgg cttggcacca
    16621 gataacggtc gaaagcggct tccagcgcat cggaaatttc acccagggtg gcgcgaacgc
    16681 gagcggcatt aacagcggca gccagcaggt tttcgttatg ctgtgcggcg tgagtcaggg
    16741 cgttcaacgc ggcggttacg gcggcatcat cacgggtggc gcgaatgcgt tccagcgaag
    16801 caatttgctc gttacgcacc atcacgttgt cgatctcaag gacgtcggtt tcgtcttcgt
    16861 gatccagttt gtacttgttg acaccaacga tgacacgctt gccctggtcg atcaacgact
    16921 gttcgcgcgc ggaagcctct tcgatcattc gttttggcag acctgcttcg atcgctttcg
    16981 ccatgccgcc ggcttcgtcg atctgttgga taatggctct ggcttgtttg acgatttgat
    17041 cggtcagcga ttcaacgtaa taggatccgg ccagtggatc gacggtgcgg cagagttctg
    17101 attcttcctg aatgatgatc tgggtgttgc gggcaatgcg tgctgagaaa tcggtaggca
    17161 aaccaagcgc ttcgtcaaag gcgttggtat gcagtgactg agtgccgccc agcgttgcag
    17221 ccagtgcttc aatggtggtg cggataacgt tgttatacgg atcctgttca gtcaggctcc
    17281 agcctgaggt ctggcagtgg gtacgcagcg ccagtgattt cggatcctgt gcgccaaatc
    17341 cactgaccgc ttcgctccat aaataacgtg ccgcacgcaa catggcgacg ttcataaaca
    17401 gatccatgcc aatgccgaag aagaacgaca ggcgaggagc gaagtcatca attttcagcc
    17461 cggcggagat tgctgctttg atgtactcaa tcccatcagc gagcgtaaat gctacctgct
    17521 gcacgcagtt ggcacccgct tcacccatat ggtaaccgct gatactgatg gtattaaatc
    17581 gcggcatgtt gccggaacac caggcgatga tgtctgcgat aatacgcatt gacggttttg
    17641 gcgggtaaat ataggtgttg cggcaaagat actctttgag gatatcgttc tgaatggtgc
    17701 cggtcagttt atcgggtgta acaccttgct cttctgcggc gacgatataa aacgccagta
    17761 ctggtagtac tgcgccattc atggtcatcg aaaccgacat tttatccagc gggatctggt
    17821 cgaacaggac tttcatatct tccacggtgt cgatagcaac gcccgctttg ccgacgtcgc
    17881 ccgccacgcg cgggttatcg gagtcgtagc ctcggtgggt ggcaaggtca aacgcaacgg
    17941 aaagaccttt ttgcccggcg gccaggttac ggcgataaaa agcgttggac tcttttgctg
    18001 ttgaaaaacc agcatactga cggatggtcc acggttgggc ggtatacata gtggcacgcg
    18061 ggccacgaac gtaggggggc aaaccaggaa gggtacctgt cacctccaga ttatcgagat
    18121 cggcttcggt atacagcggc ttgatggcga tcccttccgc ggtttgctga accagcgagt
    18181 cgacagtttt ctcccgacgg ctcaattcct tgttggcaag ctgttgccac tcctgcacgt
    18241 tagacattat ttgctccgta aaacgcaatc aaaatgaggg tatttgcgcc tacggtgagc
    18301 tttttacgtg ggtttgtcgc cagcaaaaaa aatgcattca gacgtcgttt tttgccgcct
    18361 gagatttgtc tattttgtga tgtgattcaa ggaatgcaaa aagacgtgcg gataacgcct
    18421 gatatgtgct accaggcatt atttgatgga ttaatcctga cgaaggactt tgtgaccata
    18481 atcgagtaac gcatcagtga ctttacgcat catgcggctt tcaggagcaa agcggtgcca
    18541 gtagagcatc cgtcgttgaa atagcccagg cgttaagtca atcagttcac cgctggccag
    18601 ctctttctcg atttgcaggt gcgggatcat acagcaggtg gtgccctggc gagcaagttg
    18661 cacgaacgct tctgaagaat taacgatatg gcagggtacg ctgcctgggg gcagatcgaa
    18721 gttttgctgc aaaaaggcct ggtgcatatc gtcaagatgg tcaaaggcga ccacaggtgc
    18781 tttcaataat gccgaacgcg ttacgccgtt agggaaatat ttttcggcaa acggttttga
    18841 gctgacgaac agatagtcaa gcgcaccgag cttatcgaca agacaactcg gcagcgcctg
    18901 atgttgaata ctcaccgcgc cgaccacttc gccgcggcgc agacgttcct gagtgcgggt
    18961 ttcatcttct acctgcaagt tgaggcggat aggcgaatca gccaacacag gagccagcgc
    19021 cggaagcaac cacgtcgcca gactgtcggc gttgaccgcc agtgaaagca gcagcggagt
    19081 cgaaccggtt tgttcatcgc ccagccactc ttcttccagc aactccacct ggcgtagcag
    19141 tgccagcagt ttttgccctt gttctgtcgg gcgcggcggt acggtacgca ccaacagcgg
    19201 ctgcccgaac atattttcca gttgcttaat gcgctgtgag acggctgatt gtgtaatgca
    19261 cagcttttgt gccgcgcgct caaatcctcg ttcacgtatc accgcatcca gtgcctgtaa
    19321 tgttctgtag tccgggcgtt tcattgctgt tgtctgctcc tggtaaatgc tttttcctgc
    19381 actatgacac aattttgtgt caggttgcat ataccgaagc cgtcggcggg taacatttgt
    19441 attcgtggtt aaacgcgcag cttaaaagat cagggatttg cgctaatgca tttcgctcag
    19501 cgagttcgcg ccctggttgt gttgaatggc gtggcgttat tgccccaatt tgcctgtaaa
    19561 caggggcttg cgaacggtga actggtgcgc ctgtttgcac cgtggagcgg catacccaga
    19621 ttgttgtatg ccttatttgc ggggcgaaag gggatgcctg ccattgcgcg atattttatg
    19681 gatgagttaa ccacgcggct tgccaacggg gtctgaatcg ctttttttgt atataatgcg
    19741 tgtgaaattt cataccacag gcgaaacgat catgacgcag gatgaattga aaaaagcagt
    19801 aggatgggcg gcacttcagt atgttcagcc cggcaccatt gttggtgtag gtacaggttc
    19861 caccgccgca cactttattg acgcgctcgg tacaatgaaa ggccagattg aaggggccgt
    19921 ttccagttca gatgcttcca ctgaaaaact gaaaagcctc ggcattcacg tttttgatct
    19981 caacgaagtc gacagccttg gcatctacgt tgatggcgca gatgaaatca acggccacat
    20041 gcaaatgatc aaaggcggcg gcgcggcgct gacccgtgaa aaaatcattg cttcggttgc
    20101 agaaaaattt atctgtattg cagacgcttc caagcaggtt gatattctgg gtaaattccc
    20161 gctgccagta gaagttatcc cgatggcacg tagtgcagtg gcgcgccagc tggtgaaact
    20221 gggcggtcgt ccggaatacc gtcagggcgt ggtgaccgat aatggcaacg tgatcctcga
    20281 cgtccacggc atggaaatcc ttgacccgat agcgatggaa aacgccataa atgcgattcc
    20341 tggcgtggtg actgttggct tgtttgctaa ccgtggcgcg gacgttgcgc tgattggcac
    20401 acctgacggt gtcaaaacca ttgtgaaatg atctgacggg ggaacctccc cccgttaaaa
    20461 aaattctctt cattaaattt ggtgacatgt gtcacgtttt tacctggcta ttgtcgattg
    20521 ctctaaataa accatctaaa ccagcatatt catccaagaa atacctttgc gtgatatttc
    20581 ctcaacatcg cgacgcaaac gttcatattg ccgcaatatt attttttgat atgttgaaag
    20641 gcggatgcaa atccgcacac aacatttcaa aagacaggat tgggtaaatg gcaaaggtat
    20701 cgctggagaa agacaagatt aagtttctgc tggtagaagg cgtgcaccaa aaggcgcttg
    20761 aaagccttcg tgcagctggt tacaccaaca tcgaatttca caaaggcgcg ctggatgatg
    20821 aacaattaaa agaatccatc cgcgatgccc acttcatcgg cctgcgatcc cgtacccatc
    20881 tgactgaaga cgtgatcaac gccgcagaaa aactggtcgc tattggctgt ttctgtatcg
    20941 gaacaaacca ggttgatctg gatgcggcgg caaaacgcgg gatcccggta tttaacgcac
    21001 cgttctcaaa tacgcgctct gttgcggagc tggtgattgg cgaactgctg ctgctattgc
    21061 gcggcgtgcc ggaagccaat gctaaagcgc accgtggcgt gtggaacaaa ctggcggcgg
    21121 gttcttttga agcgcgcggc aaaaagctgg gtatcatcgg ctacggtcat attggtacgc
    21181 aattgggcat tctggctgaa tcgctgggaa tgtatgttta cttttatgat attgaaaata
    21241 aactgccgct gggtaacgcc actcaggtac agcatctttc tgacctgctg aatatgagcg
    21301 atgtggtgag tctgcatgta ccagagaatc cgtccaccaa aaatatgatg ggcgcgaaag
    21361 aaatttcact aatgaagccc ggctcgctgc tgattaatgc ttcgcgcggt actgtggtgg
    21421 atattccggc gctgtgtgac gcgctggcga gcaaacatct ggcgggggcg gcaatcgacg
    21481 tattcccgac ggaaccggcg accaatagcg atccatttac ctctccgctg tgtgaattcg
    21541 acaacgtcct tctgacgcca cacattggcg gttcgactca ggaagcgcag gagaatattg
    21601 gcctggaagt tgcgggtaaa ttgatcaagt attctgacaa tggctcaacg ctctctgcgg
    21661 tgaacttccc ggaagtctcg ctgccactgc acggtgggcg tcgtctgatg cacatccacg
    21721 aaaaccgtcc gggcgtgcta actgcgctga acaaaatctt cgccgagcag ggcgtcaaca
    21781 tcgccgcgca atatctacaa acttccgccc agatgggtta tgtggttatt gatattgaag
    21841 ccgacgaaga cgttgccgaa aaagcgctgc aggcaatgaa agctattccg ggtaccattc
    21901 gcgcccgtct gctgtactaa atccccttct ctgaaaatca acgggcaggt cactgacttg
    21961 cccgtttttt tatcccttct ccacaccgtt gccgcattgc gcattctact ttgcgagtct
    22021 gctccagaaa ataaatcaaa gtcagatcaa tgcgttgtgt ttatttaatg gggatttacg
    22081 atggcagggc agcatggggc tgtaacggca aagccctcac cgaagcgagg gcttgaagga
    22141 gaagggttat gatgcgactt gtcatcatac tgattgtact gttactcata agtttcccag
    22201 cttattaaca gtcagtctca ggggaggagt catcctccct tacccttact cactaaacta
    22261 ggtcaaagaa tcaacgatgt caatcagggc gatgcaggtg tgtcgccctc accactccca
    22321 gactttcgac ggggtaacca ccgcaggcag tgggatatcc cactcttgaa cggggagttt
    22381 ttccaccaac tgacaatcat gcgcataacc caccggttgc attttatagt gctgccagtt
    22441 ttgtaaggtc cgatcataaa aaccaccgcc cattcccagg cgctgaccgt actcatcaaa
    22501 ggcgaccagc ggtgtgatca acacgtctaa tcgggaaagg ggtagcacgt cacgcacatc
    22561 cagttttggc tcatggatct tcaacctgtt catcaccagt tcgctttgcg gatggtaatt
    22621 caggaacagc aaattaccgg cactaaaggg atgcaaaact ggaagatata cgcgcttacc
    22681 ggcgcgccag agttgttcta tgagtggctg ggtgtcgagt tcgccatcaa aagagaggaa
    22741 tacagcgacc gtatgtgcca tcaccaccgg ggggtaagtc atcatccggg tagcggcttg
    22801 ttgacccatt tcctgctgtt gttccggcgt taacgcacga cgacgttgcc gaatcatttt
    22861 gcggatttct tgtcgggata atgtctgtgg gagttctggt agttgcgtca tggctggtgc
    22921 cagataagaa gggaatctcc gagatgccgc cgcaggctgt aacccttgaa cccttggttc
    22981 aaggtgaatg tgtcgtcgca gttttaaggc ttctcggacg gaccgagcat gctcaccaac
    23041 cgcggagcgc cacattcttg tggtatgaaa tatcggctca ggggactggc ccgcttgcga
    23101 acatctcaga gaaattttgt cttcacggtt actctaccac agtaaaccga aaagtgtcat
    23161 tcaaagtttt ggttagtttt ttcggtgatg cgaccttgtt caagtaacgc ttgttctatg
    23221 gtctgctgca gcatccgaat acgctgttcc atatttgccg cgtagtcacg agtcttcgct
    23281 ttttcttgcg ctaactcata gctgatattc aatgcggcaa tgaagaccaa ctgttcagta
    23341 tttgtgactc tagtgcgttc tttcagatct tgcaaccgtt ggttcagatc gtccgctgcc
    23401 tgattcaacg catccctttg gtcaggcggg cagttcacac gcagtgaacg gccaaaaatt
    23461 tggatatcga cgggttgtgc agacatgcca ccttcctgct gattgactgc gctgccttcg
    23521 tctttagacc catggtctgc gaaggggcga cactatagct accctgatga gaagagacaa
    23581 gcccttttct ggtccaccag ggcccaaagt ggtagcatat catgaatatt cctccctttg
    23641 acgacgaatg cttatgtcta tacagaacga aatgcctggt tacaacgaaa tgaaccagta
    23701 tctgaaccaa caagggacgg gtctgacccc tgctgagatg catggtttaa tcagcgggat
    23761 gatatgtggc ggtaacgatg acagctcatg gctgccgcta cttcacgacc tgacgaacga
    23821 aggcatggct ttcggtcatg agctggcaca ggcactgcgt aaaatgcact ctgccaccag
    23881 cgatgccctg caggatgacg gcttcctttt tcagctttat ctgcctgatg gcgatgatgt
    23941 cagcgttttt gatcgggctg atgcactggc aggttgggtc aatcacttcc tgcttggtct
    24001 tggcgttacg caaccgaagc tggacaaagt gaccggagaa accggtgaag ccatcgacga
    24061 tctgcgtaac atcgcgcagt tgggctacga cgaagacgaa gatcaggaag agcttgaaat
    24121 gtcgcttgaa gagatcatcg agtacgtccg tgttgccgcg ctgttatgcc acgacacctt
    24181 tactcatccg caaccgaccg cgccagaagt acaaaaaccg actctacact aaaaacaaaa
    24241 aacgtaagga gagtgttatg agtgatatat cccggcaaga gtttcagcgt cgccgtcagg
    24301 ccctggtgga gcaaatgcaa cccggcagcg ccgcgctgat ttttgctgca ccagaagtaa
    24361 cacgtagcgc cgacagcgaa tacccctatc gtcagaacag tgacttctgg tacttcaccg
    24421 gctttaatga accggaagcg gtgctggtgc tgattaaaag cgatgacact cataaccaca
    24481 gcgttttgtt taaccgcgtt cgcgacctga cggcagagat ctggtttggt cgtcgcttag
    24541 gccaggatgc cgcgccagag aaactgggcg ttgaccgcgc actggcattc agtgaaatca
    24601 atcagcaact ttatcaacta cttaacggcc tggatgtggt ttaccatgct cagggcgaat
    24661 atgcatatgc tgatgaaatc gtgaacagtg cgctggaaaa actgcgtaaa ggctcgcgac
    24721 aaaatctcac cgcgccagca acgatgatcg actggcgtcc cattgttcat gaaatgcgcc
    24781 tgttcaaatc gccggaagag attgccgtac ttcgccgcgc gggtgaaatc accgccctgg
    24841 cgcatacccg ggcgatggaa aaatgccgtc cgggaatgtt cgagtaccac ctggaaggcg
    24901 aaattcacca cgaatttaac cgccacggcg cgcgctatcc ttcctataac accattgtcg
    24961 gcagcggtga aaacggttgc attctgcact ataccgaaaa cgagagtgaa ctgcgtgacg
    25021 gtgacctggt gttgattgat gcgggttgcg aatacaaagg ttacgctggc gatattaccc
    25081 gcaccttccc ggtcaacggc aaattcactc aggcccagcg tgaaatctac gacattgtgc
    25141 tggagtctct tgaaaccagc ctgcgcctgt atcgtccggg aacttccatg caggaagtca
    25201 ctggtgaagt ggtgcgcatc atggttagcg gcctggtaaa actcggcatc ctgaaaggtg
    25261 atgttgatga actgatcgct cagaacgccc atcgtccttt ctttatgcat ggccttagcc
    25321 actggttagg actggatgtc catgacgttg gcgtttatgg tcaggatcgc tcgcgcattc
    25381 tggaaccggg gatggtactg accgtagagc ctggtctgta tatcgcgccg gatgcggatg
    25441 tgccagaaca atatcgcggt atcggcattc gtattgaaga cgacattgtg attaccgaaa
    25501 ccggcaacga aaacctcacc gccagcgtgg tgaaaaagcc ggaagaaatc gaagcgttga
    25561 tggctgctgc gagaaagcaa tgagcgtaat catcgtcggt ggcggcatgg cgggcgcgac
    25621 gctggcgctg gctatttccc ggttaagtca cggggcgctg ccggtacatt tgattgaagc
    25681 gactgcgcca gagtcacatg ctcatccggg ctttgatgga cgagctattg cgctggcggc
    25741 gggtacctgt cagcaactgg cgcgcatcgg cgtctggcaa tctctggctg attgcgcaac
    25801 cgccatcacc agcgtgcatg tcagcgatcg tggtcatgcc ggattcgtca ccctcgccgc
    25861 agaagattac caactggcgg cgctggggca ggttgtcgaa ttgcataatg tcgggcaacg
    25921 gctgtttgca ttgctgcgca aagcacctgg cgtaacgctg cattgcccgg atcgcgtggc
    25981 taacgttgcc cgtactcaga gtcacgttga agtgacgctg gagagtggcg agacgctgac
    26041 gggctgcgtg ctggtagccg ctgatggcac ccattcagcg ttagccactg tgtgcggcgt
    26101 tgactggcag caggagcctt acgaacaact ggctgtgatt gccaacgttg cgacttccgt
    26161 tgcgcacgaa gggcgcgctt ttgaacgctt tacgcaacat ggcccgctgg cgatgttgcc
    26221 gatgtctgac ggacgctgtt cgctggtctg gtgtcatcca ctggaacggc gcgaagaggt
    26281 actgtcgtgg agcgacgaga agttttgccg tgaactccag tcggcctttg gttggcgact
    26341 tgggaaaatt acccacgctg gtaaacgcag tgcttatccg ctggcgttaa cccgcgccgc
    26401 cagacctatt acccatcgta ccgtgctggt gggcaatgcg gcgcaaactc tgcacccgat
    26461 tgccgggcaa gggttcaacc tcggtatgcg agatgtcatg agcctagcgg aaaccctgac
    26521 tcaggcgcag gagcgcggag aagacatggg tgattacggc gtattgtgcc gttatcagca
    26581 gcgtcgacag cgcgatcgcg aagcaaccat cggcgtcacg gacagccttg tacatctttt
    26641 tgccaaccgt tggaccccgc tggttgtcgg gcgcaacatc gggctgatga cgatggaatt
    26701 atttaccccg gcacgcgatg tgctggcgca gcgcaccctc ggttgggtgg cgcgttgaag
    26761 gcggttttaa ggaataaaca atgcaaagtg ttgatgtagc cattgttggc ggcggcatgg
    26821 tggggctggc ggttgcctgc ggcttacagg ggagcggctt acgcgttgcc gtactggagc
    26881 agcgcgtacc ggaacctctg gcggcgaatg caccaccaca actgcgcgtt tcggctatca
    26941 atgccgccag cgaaaaatta ctcacccgtc ttggcgtctg gcaggacatt ctctctcgta
    27001 gggccagttg ttatcacggt atggaagtgt gggacaaaga cagttttggt cacatttcgt
    27061 ttgacgatca aagcatgggc tatagccatc ttgggcatat cgttgaaaac tcagtgattc
    27121 actacgcgct gtggaacaaa gcgcagcagt cgtcagatat cactctgttg gcccccgcag
    27181 aattacagca ggtcgcctgg ggagaaaacg aaaccttcct gacgctgaaa gatggcagca
    27241 tgttaacggc gcgtctggtg attggcgcgg acggcgctaa ttcctggttg cgcaacaaag
    27301 ccgatattcc gctgactttc tgggattatc agcatcacgc gctggtagcg accattagca
    27361 cggaagaacc gcatgatgcg gtggcgcggc aggttttcca tggcgaaggc attctggcct
    27421 ttttaccgct tagcgatccg catctttgct cgattgtctg gtcactgtcg ccagaggaag
    27481 cgcagcggat gcagcaggca agtgaagacg aatttaatcg cgcgttaaat atcgcttttg
    27541 ataatcgcct gggcttatgt aaggttgaga gtgagcgcct tgtgttccca ctgacggggc
    27601 gttatgcgcg ccaatttgcc gcgcaccgtc tggcgttggt gggcgacgcc gcgcatacca
    27661 ttcacccgct ggcggggcag ggggtaaatc tcggctttat ggatgctgca gagctggttg
    27721 ctgaactgaa acggttgcat cgtcagggga aagacatcgg gcagtacatt tatctgcgtc
    27781 gctatgagcg tagccgcaag cacagtgcgg cgctgatgct ggctggtatg cagggatttc
    27841 gcgatctgtt ttccggtacc aatccggcga aaaaactgct gcgtgatatt ggtctgaaac
    27901 tggccgacac gcttcctggc gttaaaccac aacttatccg ccaggcaatg ggattaaacg
    27961 atttgcctga atggctgcgt taaaaatttc tcttctgctg tttatttgat acccatcaca
    28021 ctttcatctc ccggtttttt cgccgggaga ttttcctcat ttgaaataaa ctaatttcac
    28081 ctccgttttc gcattatatt ttctaatgcc acgaattttt gatttagtgt tttttgacat
    28141 ttttttagtt ct
//
LOCUS       DACBVI010000048        27956 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.048, whole genome shotgun sequence.
ACCESSION   DACBVI010000048 DACBVI010000000
VERSION     DACBVI010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.048"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            73..148
                     /locus_tag="IU325_10180"
     tRNA            73..148
                     /locus_tag="IU325_10180"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:106..108,aa:Lys,seq:ttt)
     gene            complement(259..1143)
                     /locus_tag="IU325_10185"
     CDS             complement(259..1143)
                     /locus_tag="IU325_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP5809389.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVDVLFREKMPAMQMALLERRELDAGIWRMATEPPA
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALGDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKERIPADLYIVY
                     ETQQVTPALVKLLAALAQ"
     gene            complement(1184..1342)
                     /locus_tag="IU325_10190"
     CDS             complement(1184..1342)
                     /locus_tag="IU325_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XapX domain-containing protein"
                     /protein_id="HAP5809390.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            complement(1395..2651)
                     /gene="xapB"
                     /locus_tag="IU325_10195"
     CDS             complement(1395..2651)
                     /gene="xapB"
                     /locus_tag="IU325_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HAP5809391.1"
                     /translation="MSIALRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVSDPDTMFWVMLIN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYVASGASLLLSAYALTLPKIPVAEKKSATSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMMMSMVAWTLRFGFFAYGDPSPTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKITHRSVTH"
     gene            complement(2711..3544)
                     /gene="xapA"
                     /locus_tag="IU325_10200"
     CDS             complement(2711..3544)
                     /gene="xapA"
                     /locus_tag="IU325_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HAP5809392.1"
                     /translation="MSQARFSHNPLYCVDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDADYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHTQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            3793..4557
                     /locus_tag="IU325_10205"
     CDS             3793..4557
                     /locus_tag="IU325_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809393.1"
                     /translation="MKKHLFTLTLSSVLAIPAVTHAEFKGGFADIGIHYLDWTSRTTE
                     KSSTKSHKDDFGYLELEGGANFSWGEMYGFFDWENFYNDRHDKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYSFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSYITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(4595..5521)
                     /locus_tag="IU325_10210"
     CDS             complement(4595..5521)
                     /locus_tag="IU325_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP5809394.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFILHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAQALWDVVRGQGRALMAGREGDPLYQI"
     gene            5611..6609
                     /locus_tag="IU325_10215"
     CDS             5611..6609
                     /locus_tag="IU325_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HAP5809395.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKHQTEQLQAQQESRANKA"
     gene            complement(6606..6824)
                     /locus_tag="IU325_10220"
     CDS             complement(6606..6824)
                     /locus_tag="IU325_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809396.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(6826..8841)
                     /gene="ligA"
                     /locus_tag="IU325_10225"
     CDS             complement(6826..8841)
                     /gene="ligA"
                     /locus_tag="IU325_10225"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP5809397.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGILEGGELPNTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLTQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPVVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(8912..9910)
                     /gene="zipA"
                     /locus_tag="IU325_10230"
     CDS             complement(8912..9910)
                     /gene="zipA"
                     /locus_tag="IU325_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HAP5809398.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVQQPAYQPQPEQPLQQPVSPQVASAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            10140..10901
                     /gene="cysZ"
                     /locus_tag="IU325_10235"
     CDS             10140..10901
                     /gene="cysZ"
                     /locus_tag="IU325_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HAP5809399.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            11086..12057
                     /gene="cysK"
                     /locus_tag="IU325_10240"
     CDS             11086..12057
                     /gene="cysK"
                     /locus_tag="IU325_10240"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP5809400.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            12441..12698
                     /gene="ptsH"
                     /locus_tag="IU325_10245"
     CDS             12441..12698
                     /gene="ptsH"
                     /locus_tag="IU325_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HAP5809401.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            12743..14470
                     /gene="ptsI"
                     /locus_tag="IU325_10250"
     CDS             12743..14470
                     /gene="ptsI"
                     /locus_tag="IU325_10250"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HAP5809402.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            14511..15020
                     /locus_tag="IU325_10255"
     CDS             14511..15020
                     /locus_tag="IU325_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP5809403.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(15062..15913)
                     /gene="pdxK"
                     /locus_tag="IU325_10260"
                     /gene_synonym="thiJ"
     CDS             complement(15062..15913)
                     /gene="pdxK"
                     /locus_tag="IU325_10260"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HAP5809404.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            16018..16386
                     /locus_tag="IU325_10265"
     CDS             16018..16386
                     /locus_tag="IU325_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809405.1"
                     /translation="MKKIICLVITLLMTLPVYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVSAQ"
     gene            complement(16389..17300)
                     /gene="cysM"
                     /locus_tag="IU325_10270"
     CDS             complement(16389..17300)
                     /gene="cysM"
                     /locus_tag="IU325_10270"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HAP5809406.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(17434..18531)
                     /gene="cysA"
                     /locus_tag="IU325_10275"
     CDS             complement(17434..18531)
                     /gene="cysA"
                     /locus_tag="IU325_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HAP5809407.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEALTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(18521..19396)
                     /gene="cysW"
                     /locus_tag="IU325_10280"
     CDS             complement(18521..19396)
                     /gene="cysW"
                     /locus_tag="IU325_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HAP5809408.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(19396..20229)
                     /gene="cysT"
                     /locus_tag="IU325_10285"
     CDS             complement(19396..20229)
                     /gene="cysT"
                     /locus_tag="IU325_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HAP5809409.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRRQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(20229..21245)
                     /locus_tag="IU325_10290"
     CDS             complement(20229..21245)
                     /locus_tag="IU325_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP5809410.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(21416..22207)
                     /gene="ucpA"
                     /locus_tag="IU325_10295"
     CDS             complement(21416..22207)
                     /gene="ucpA"
                     /locus_tag="IU325_10295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HAP5809411.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(22336..23193)
                     /gene="murR"
                     /locus_tag="IU325_10300"
     CDS             complement(22336..23193)
                     /gene="murR"
                     /locus_tag="IU325_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HAP5809412.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     TDSPLRRLAHYTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIER
                     SSELTQRLK"
     gene            23357..24253
                     /gene="murQ"
                     /locus_tag="IU325_10305"
     CDS             23357..24253
                     /gene="murQ"
                     /locus_tag="IU325_10305"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP5809413.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCSAEQAEAALVACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            24257..25681
                     /gene="murP"
                     /locus_tag="IU325_10310"
     CDS             24257..25681
                     /gene="murP"
                     /locus_tag="IU325_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HAP5809414.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPTATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            25686..26987
                     /locus_tag="IU325_10315"
     CDS             25686..26987
                     /locus_tag="IU325_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HAP5809415.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFNAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQ"
     gene            complement(27045..27944)
                     /gene="yfeX"
                     /locus_tag="IU325_10320"
     CDS             complement(27045..27944)
                     /gene="yfeX"
                     /locus_tag="IU325_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HAP5809416.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
BASE COUNT         6793 a         7550 c         6914 g         6699 t
ORIGIN      
        1 agctcagctg ggagagcacc tcccttacaa ggagggggtc ggcggttcga tcccgtcatc
       61 acccaccact tcgggtcgtt agctcagttg gtagagcagt tgacttttaa tcaattggtc
      121 gcaggttcga atcctgcacg acccaccaat gtaaaaaagc gccctaaagg cgcttttttg
      181 ctatctgcaa tttgcgaaat tgcctgatgc gcttcgctta tcagaccaac tatgtccggc
      241 agttcccgtg cctctcatct actgcgccaa cgccgccagc agtttaacca gcgcgggcgt
      301 cacctgctgc gtttcataaa caatatataa gtctgccggg atgcgttctt tgagcggacg
      361 gaatatgaca ccgggccagt tcatctgcgc gtagctgtcc gctatcaatg tgataccaat
      421 acccatactg accatggcga gtaccgtttg cggttcatta acttcgcgaa taacaaccgg
      481 tgaaaatccc acctgctggc aaactcgctg caaaaaatcc cagtcagtgt acacgggcgg
      541 cattgtaaca aaatactcgt cacctagcgc ttccagcgga acggtggaaa atgatgagag
      601 atgatgctct tcaggcatcg ccaccagaaa cgccgattca tgcaaccgta agctggtaaa
      661 accagccggt ggttctgtcg ccattctcca gattccggca tcgagttcac gccgttccag
      721 cagggccatt tgcatcgcgg gcatcttttc gcgaaaaaga acgtcaacgt taggattttc
      781 cctgaggaat cgccgcataa ccgggcgcat ccgtccccac attgccgttc ccacaacccc
      841 gagttcaatc cgccctgctt ctccccgacc tatttgttca acccgagcca atacattatt
      901 tgcattcacc agcaatcgac gcgattcttc catcaagatt ttgcccgcgt gtgtcagtac
      961 gacgctgcgc gaatggcgaa taaaaagctg cgtgccaagt tgattttcca gctctttaat
     1021 atgaatactg agcggaggct gagacatatt taaacgcgct gctgcgcgac caaaatgcaa
     1081 ctcttccgct acggcaagaa aataacggag caacttaaga tctgttctat atacgcgttc
     1141 cataattaag tgctccatat aataaataca tcgtattaaa ttatcatggc ataaagtatt
     1201 tcaccaccag atatccgaca tacataaaca cgatggataa tatacccggc aatacatttg
     1261 gaacaggaat aggtaatcgc acgattgtaa acaaagcacc aatgccaaaa ccaacgccag
     1321 aagaaaataa aatatctttc actaattaac ctttatcata aaagcagccc caaggaacag
     1381 agctgcgaat ccttttaatg tgtaactgaa cgatgcgtta tcttttcagg gtcatgatta
     1441 tatttaaacc caaagaaaaa tatcactgcg agaaaaagag catatcctgc aaacaccagc
     1501 cagatagttt gccagtcttt tacgccatcc accgaaaagt aatcaactgc catgccactc
     1561 agaatcgagc caacccatgc gccgacacca tttaccatgg tcataaagag cccctgcgcg
     1621 ctggcacgaa tgctggaatc aacttcctgt tcgacaaata ccgaaccaga aatattgaag
     1681 aaatcgaatg cacagccata aacaatcatc gacagcagca gcaaaataaa cccggttggt
     1741 gacggatctc cataagcgaa gaaaccaaag cgcagcgtcc aggccaccat actcatcatc
     1801 atgacggttt tgatgccaaa acgcttcaaa aagaacggaa tcgtcaaaat gaaaccgact
     1861 tcagccatct gtgaaactga cagtaaaatg gagggatatt tcaccacgaa gctatcagca
     1921 aactccgggt tacgggcgaa atcatgcagg aacggattac caaaaacgtt ggtaatttgc
     1981 agtaccgcac ccagcatcat ggcaaagagg aaaaagatgg ccatgcgtgg atttttaaac
     2041 agcacgaagg catccagacc cagcttgctg gcaagcgacg tggccgattt tttctccgca
     2101 accggaatct taggcaaagt cagcgcataa gccgacagca acaatgacgc accggacgca
     2161 acatacagct gcaggctgct caattccaaa tgcagtaagc ttaccgccca catcgcgaca
     2221 atgaatccca ccgtaccaaa aacgcgaatg ggtgggaaag ccgtcaccgg atcaagccct
     2281 gcctgggcca ggcaagagta agagacactg ttcgataatg cgatagtcgg cataaatgcc
     2341 atcgcattga ttaacatcac ccaaaacatc gtatccggat cactcaccga cgccgcataa
     2401 aaaagtacgc ccgcgcacac cagatggcac agcatatatg ctcgttcagc gcgcagccat
     2461 ttgtccgcga tgatccccat aatgccaggc ataataatcg cggcaatacc tttggagctg
     2521 taaaccatgc cgacattagc gccggtaaaa tggagggtat taatcatgta agaaccgagg
     2581 gtaaccagcc agctccccca gataaaatat tgcaaaaagg acattacctt taagcgcaaa
     2641 gcgatgctca taatcgtttc cttgtcgcat catgtggcct cacccgtgag gccgtttatt
     2701 gttgttttaa tcaggcaatt ttgcgcagaa acccgcaaat taggttgata aagttctgct
     2761 tagaaagctc cgctgctgcc agcgtttggg tatgcgagag cttcacgtca ctcaggcctt
     2821 ctgccatatt ggtaatcgca gaaacggcca ctaccttcag gtcacaatgg cgtgctgaaa
     2881 taacttcagg cacaacggac attccaacga catccccgcc aataatttgc atcatgcgaa
     2941 tttcagccgc cgtttcgaaa ttcggccccg gatacgaaac aaacacacct tccgtcaggg
     3001 ggaatccctc ttctttcgcc actttttgca acagcgcccg gtaatccgca tcataggcat
     3061 ttgccagtga gaagaaacgc tctccaaaac gatcatcgtt aagtcccacc atcggcgtac
     3121 ctggcatggt gttgatatga tctttcaatg cgaccaggct gcctgccccc acttccggac
     3181 gcagcgagcc tgccgcattg gtgcagaaca gtagctcgca gcccagcaac ttaaaggtac
     3241 gaattgcgtc agtcataatg gtcattccac gaccttcgta gaaatgtccg cgacctttca
     3301 tacataccac cggtacgccc tggagatggc ccagcactaa ctcccccgca tgaccatgta
     3361 ccgtacttac cgggaagccc ggcagctttt cgtaggaaat tgcgaccgcg ttctcaatct
     3421 gatcggccag cgcgcccagc ccggaaccta aaataaaggc cactcgtggc gtgaaatcag
     3481 gtttataagt cttgataata tctacgcaat acagtgggtt atgagaaaac cgagcctgag
     3541 acatatatat cctttttttg tagggtggaa tctaacgcct ttttataacg agattgccct
     3601 acctcatacc aatacacttt tttcatgtat ccgatagtta ttcgatatcg atatatttat
     3661 attttttcac tattaatagg cggaaaaaac tgtattggta ctctcctttc cattgcatta
     3721 acaatatgcc gcttttaatc aaccttttta ttaagcaatc aactaataac gatcggcata
     3781 gggaattatg ctatgaaaaa acatcttttt actctgacac tctcttctgt attggcgata
     3841 ccagcagtaa cccatgcaga atttaaaggc ggttttgctg atataggtat tcattatctt
     3901 gactggacta gcagaaccac tgagaaatcg tcgaccaaat cacacaaaga tgattttgga
     3961 tatctggaac tggaaggcgg cgctaacttt agttggggag aaatgtacgg gttcttcgac
     4021 tgggaaaact tttataatga tcgtcatgat aaaccgggta gcgagcagcg ttatacattt
     4081 aaaaatacca accgtattta cctgggtgac accggattta atctctactt gcatgcgtat
     4141 ggcacctacg gttctgcgaa tcgtgtgaat ttccatgacg atatgttcct ttacgggatc
     4201 ggctacaatt tcaccggcag cggttggtgg ttcaaaccgt tctttgcaaa acgctataca
     4261 gaccaaacct attataccgg cgataacggt tatgtcgccg gttgggttgc aggttacagc
     4321 tttatgctgg gcagtgagaa attcaccctc accaactgga acgagtacga gtttgatcgt
     4381 gatgccacct acgcggcagg taatggcggc aaagaaggtc tgaacggtgc cgtcgcactt
     4441 tggtggaatg caacttcata catcaccaca gggattcagt atcgttatgc ggatgacaaa
     4501 ctgggcgaag atttttacca ggatgcgata atctactcca tcaaatttaa tttctgattg
     4561 attactgccg gggctcccgg cagtatgaat agctctatat ctgatacagc ggatcccctt
     4621 cccgcccagc cattagcgca cggccctggc ctcgtaccac gtcccataac gcttgtgcgg
     4681 cggtggaaag tgaacggttc ttcctgcgca ccagcatcaa ctggcgttcc accaccggcg
     4741 taatgcgctt taccaccagc gggctacctt caggcaatgg tagcgccaga gcgggaagaa
     4801 tactgatgcc gatgcctgcg gctaccatcg ggaacagcgt cgccggatga ccaatctcct
     4861 gcacaatatt cgcctgaata ccgttgcgcg ccagagctgc gtcaatcagc ggtcggctgc
     4921 ctgacgcgta atcctgcaac accaatttcg ctccctgtaa tgcctgccag ggtacataat
     4981 cttccgcggc caaagcgcta tcgcgatggc aaagcaagaa gaaaggctcg gaaagaatcg
     5041 cttcacattg cagatcgccc actgggcctg gatcgatgac gatgccaaaa tcgacatctc
     5101 cctggcgaat actttccatc acccactgct gcggacgatc gtgcaggata aactgaatat
     5161 ctggatagcg gcgatggctt tcagcaatgc attgcggaat aagatgcgcg gaaatggtct
     5221 ggctggcagc gacccgaact tttccgctca gttgttgccc catacgcccg gtatcgcgca
     5281 acgtgctgtt aagttcatcc agcagtcgct caagacgcaa agccaactgc tgccctgcat
     5341 ctgtaagcac cacttcacgc gtggttctgt ccagcaggcg aacaccagta tgattttcca
     5401 gctccttcac actgtgactt actgccgact gactcaggcc gatacgctct cctgcacgac
     5461 taaaactttt ctcctgcgct actgtgacga aaacctttaa ttgctttaaa gaataattca
     5521 tctgttttct tcatgaatgg atgcaataaa tcaattttat ttctcaaact ggaagaagca
     5581 caatagaacc atcgatcatc tggagtcttt atgaaacttt ttcgtatcct cgatcctttc
     5641 accttaaccc tgatcacggt ggtgttgctg gcctctttct ttccggccag aggcgatttc
     5701 gtccccttct ttgaaaatct gaccaccgca gctattgccc tgctgttctt tatgcacggc
     5761 gcgaagttgt cgcgtgaagc gattattgct ggcggtggtc actggcgact gcatttgtgg
     5821 gtaatgtgca gcacctttgt actgtttccg attctgggcg tgctgtttgc ctggtggaaa
     5881 ccggtaaatg tcgacccgat gctctactcc ggttttctct acttgtgcat tctccccgct
     5941 accgtgcagt ctgcaatcgc ctttacttca atggcgggcg gtaacgtcgc ggcagcggtt
     6001 tgttctgcgt cagcatccag cctgttgggg attttccttt caccattgct ggttggtctg
     6061 gtgatgaatg ttcacggtgc gggaggcagc cttgagcagg tcggtaaaat tatgctgcaa
     6121 ctgctgctgc cgtttgtgtt ggggcatctt tcccggccgt ggattggtga ctgggtgtcg
     6181 cgcaataaaa aatggattgc gaaaactgac cagacgtcca ttctgttggt ggtttataca
     6241 gcgtttagcg aagccgtcgt taatggtatc tggcataaag ttggctgggg atcattgctg
     6301 tttatcgtgg tggtcagctg cgttcttctg gctatcgtga ttgtagttaa cgtctttatg
     6361 gcacgccgac tgggcttcaa taaggcagat gaaattacta tcgtcttttg tggttcgaaa
     6421 aagagtctgg caaatggcat cccgatggca aacattcttt tccccacatc ggtgatcggt
     6481 atgatggtgc tgcctctgat gattttccat cagatccaat tgatggtctg tgcggtgctg
     6541 gcgcgtcgat acaaacacca gaccgaacag ttacaggcgc agcaggaaag ccgcgccaat
     6601 aaagcttaaa gaggacgctt caggggctgg accagttgcg tcagcccctc agttttaatc
     6661 agcagcgtga tttgcattag ctcaccgagc ttccctgctg ggaactcatc tttgcgggca
     6721 aaccacagaa gatactcctc cggcaggtcg attaagcgac gccctttgta tttgccaaac
     6781 ggcattatcg tattggctat ttcaatcagc tgctcttttt ccatctcagc tacccagcaa
     6841 acgcagcatt tccgcttcgt cgataacttc tatgcccagt tcctgcgctt tcgccagttt
     6901 agaacctgcc gcttctccag ctatcaccag atcggttttc ttcgacacgc tgcccgcgac
     6961 tttcgcgccc agttcgacca gtcgagcttt agcgtcatca cgtgacatct ggcttaagct
     7021 gcccgtaagc accacggttt taccagcaaa cgggctgtca atctcttccg cgttaatcac
     7081 caccggcgca ggccagtgaa cgccttccgc caacagctcg ctgatgacat tgcggttgct
     7141 ttcttcggca aagaagttgt gaacgtggga tgcaacgaca atgccaacat caggcacctt
     7201 ttgcagctct tcaatcgaag cggcttccag cgcttccagc gtgccgaaat atgccgccag
     7261 acctgctgcg gtggcctcgc cgacttcacg gatgccaagt gcatagagga agcgagcaaa
     7321 ggtggtttct ttcgcttttt ccagcgcatt aaccacgttt tgtgccgatt ttggccccat
     7381 acgctccagt ccggtaagtt tgcctgcggt gagtttgaac agatccgccg gagtgtggac
     7441 atattctttt tcaaccagct gatcgatgat tttgtcgccc ataccgtcaa cgtccatcgc
     7501 acggcgggaa acaaagtgtt tcagcgactc tttacgctgc gcaccgcaaa tcaggccacc
     7561 ggtacagcgg gcaaccgctt caccttccac acgctcaacg tcagaaccac ataccggaca
     7621 atgcgtcggg aatacaacct cacgggtatc ttccgggcgt tcagaaagca cgacgttaac
     7681 cacctgcggg atcacgtcgc cagcgcggcg aatcaccact ttatcgccaa tgcgtaaacc
     7741 aagacgttcg atttcatccg cattgtgtaa ggttgcgtta ctcaccagca cgcctgcaac
     7801 atggacaggt tccagacgcg caacaggcgt aatcgcgcca gtacgcccaa cctgaaactc
     7861 gacgtcacgc acaaaggtca tctgttcctg cgccgggaat ttaaacgcta ccgcccagcg
     7921 cggggcacgc gcgacaaagc caagctgctc ctgctgtgtc agtgagttga ccttaatcac
     7981 cacgccgtcg atatcaaagc ccagcgtcgg gcggtcttct tccactttgt gatagaacgc
     8041 cagcacttct tccgccgatt cacaaagtgt tacccggtcg ctgaccggca aaccccactt
     8101 tttaaattgc agtaaacggc caagatgagt attcggcagc tcgccacctt ccagaatacc
     8161 gacgccatag cagaaaaaag tgagcggtcg cttcgctgta atacgcggat caagctgacg
     8221 cagtgaacca gcagccgcat tacgtgggtt agcaaacact ttcccgcccg tgcgtcgcgc
     8281 atcttcgtta atcttttcga accccgcctg cggcaggaac acttcaccac gcacttccag
     8341 acgcgccggg atattctctc cgtgtagctt cagcggaatg gcgcgaatag tacgcacatt
     8401 agacgtaata tcttccccgg tagtgccatc gccacgcgtc gcggcactga ctaaaacgcc
     8461 attttcatac agaatactga cggcaagacc atccagcttc agctcacagc accaggtgac
     8521 tttctcgttg cttttcagac ggtcctgcac acgtttgttg aaagcaagaa agctttcttc
     8581 atcaaaaacg ttatccagcg acagcattgg cacttcatgg cggatctggc tgaaagccgc
     8641 cagcggcgca gcacctacac gttgggtagg cgaatcaggc gtaatcagtt ctggatgttt
     8701 ggtttccagc tcgcgcagtt cgcgcatcag cctgtcgtat tcagcgtcgg gaatttccgg
     8761 cgcatccatc acatgataaa gatattcatg atggcgaagc gtcgttcgca gttctgtcag
     8821 ttgttgttcg attgattcca tatcgcacca tcaatgctaa aaacccccga caagcggggg
     8881 ttcgaagagg agttaatttg ccttaagtgt atcaggcgtt ggcgtctttg acttcgcgga
     8941 tgatgtcctg gtactcgcgc aatttctgcg gagtcatcat acggcgctga tcgtcaagca
     9001 ctacaccgcc cacttcatcg gcaatatgct gcgcagattg cagcatcagc ttgaagttct
     9061 gcagctcgtc accgtaagac ggtacctgca taaagatagt gacacccgga gtagtgaaat
     9121 ccttcatttc aggatcaaag gttcccggtt tcaccatatt cgccaggcta aataacgccg
     9181 ggccgctgcc atccgggcta agatgacgat ggtaaatatt catatcgcca aaaatgaagc
     9241 ccgcttgttg aatgctgtta agaagcagtt caccgtttag ctcgctaccg tgatgcgccg
     9301 cgacgttcat gataatcacc gcttctttgc gcttcggttt atccataact ggagccggtt
     9361 ccgctacagg ctcaggctgt ggtgccgcta caggctctgc aggctggaaa gcctgttgtg
     9421 ccggttgcgg tgctgaatgc actggctgcg gcgctgatgc gacctgtggc gaaactggct
     9481 gctgcaacgg ttgttcaggc tgcggctgat aggcaggctg ctgcacgggt tgctgcaccg
     9541 gctgcgctgg acgcggagca tgttgcggcg gtacctgcgc ttcaggcggc tgctggaccg
     9601 gttgacgcgg ctgcgcagac gcataaggcg gttggtactg gtgttgcggc gacggacgag
     9661 cagcctcatg ctcctgggcg ttagccgggg catgattcac gcggtgaaca cgaacctcac
     9721 caacgccctc atcatcttcg acatcctcgt cataagaatc gtcgtcacgt tttgacttca
     9781 ttcgttttaa tggccgatcg cggaacatag aagatcgttc tttacggctg gtccagaaac
     9841 catgtaccag taaagcgatt atggcgatcg cgccaacaat gattaatatc agacgcaaat
     9901 cctgcatcat tatattctct gttgttctaa caccttgcca ccacggcaaa catttactca
     9961 ctaagagtat ttgccgatta cctcaagtgc aagtgcacta ttaactttca cagcacaaag
    10021 atagatgaaa tcgtgctttt tgctgttttt tcgaacatat cctaactgtc cattgcgcaa
    10081 ttacccggta aaatacgcag aattttcctg ggattggtca aaaggagctc atcctgatta
    10141 tggtttcatc attcacatct gccccacgca gcggttttta ctattttgcg caaggctgga
    10201 agctcgtctc gcaacctggg attcggcgtt tcgttatttt accgctgctg gtcaatattt
    10261 tgttgatggg gggcgcattc tggtggctct ttacgcaact cgatgtctgg atcccgactc
    10321 tcatgagtta tgttccggac tggttacaat ggctgagcta tcttttgtgg cctctggcag
    10381 tcatctccgt gctgttagtg tttggctatt tcttctcaac gattgctaac tggattgccg
    10441 ctccgtttaa cggtttattg gctgaacaac tggaagcacg attgactggc gctacaccgc
    10501 cagataccgg gattttcggt atcatgaaag atgtgccgcg aatcatgaaa cgcgaatggc
    10561 aaaaatttgc ctggtatctg ccgcgcgcaa ttgtattgct aattctttac ttcatccctg
    10621 gtattgggca aaccgtcgcg ccggtactgt ggttcctgtt tagcgcctgg atgttagcca
    10681 tccagtattg cgattacccc ttcgataacc acaaagtgcc gtttaaagag atgcgcaccg
    10741 ccctgcgcac acgcaaaatc accaatatgc agtttggtgc tttaaccagc ctgtttacga
    10801 tgatccctct gcttaatctg ttcatcatgc ccgttgccgt ttgtggtgcg acggcgatgt
    10861 gggtcgattg ctatcgcgat aaacacgcga tgtggcggta acaatctacc ggttattttg
    10921 taaaccgctt gtgtgaaaca ggggtggctt atgccgcccc ttattccatc ttgcatgtca
    10981 ttatttccct tctgcatata gatatgctaa atccttactt ccgcatattc tctgagcggg
    11041 tatgctacct gttgtatccc aatttcatac agttaaggac aggccatgag taagattttt
    11101 gaagataact cgctgactat cggtcacacg ccgctggttc gcctgaatcg catcggtaac
    11161 ggacgcattc tggcgaaggt ggaatctcgt aaccccagct tcagcgttaa gtgccgtatc
    11221 ggtgccaaca tgatttggga tgccgaaaag cgcggcgtgc tgaaaccagg cgttgaactg
    11281 gttgaaccaa ccagcggtaa taccgggatt gcactggcct atgtcgctgc cgctcgcggt
    11341 tacaaactta ccctgaccat gccagaaacc atgagtattg aacgccgcaa gctgctgaaa
    11401 gcgttaggtg caaacctggt gctgaccgaa ggtgctaaag gcatgaaagg cgcaatccaa
    11461 aaagcagaag aaattgtcgc cagcaatcca gagaaatacc tgctgctgca acaattcagc
    11521 aatccggcaa accctgaaat tcacgaaaaa accaccggtc cggagatctg ggaagatacc
    11581 gacggtcagg ttgatgtatt tattgctggc gttgggactg gcggtacgct gactggcgtc
    11641 agccgctaca ttaaaggcac caaaggcaag accgatctta tctctgtcgc cgttgagcca
    11701 accgattctc cagttatcgc ccaggcactg gctggtgaag agattaaacc tggcccgcat
    11761 aaaattcagg gtattggcgc aggttttatc ccggctaacc tcgatctcaa gctggtcgat
    11821 aaagtcattg gcatcaccaa tgaagaagcc atttctaccg cccgtcgtct gatggaagaa
    11881 gaaggtattc ttgcaggtat ctcttctgga gcagctgttg ccgcggcgtt gaaactacaa
    11941 gaagatgaaa gctttaccaa caagaatatt gtggttattc taccatcatc gggtgagcgt
    12001 tatttaagca ccgcattgtt tgccgatctc ttcactgaga aagaattgca acagtaatgc
    12061 cagcttgtta aaaatgcgta aaaaagcacc ttttcaggtg cttttttgtg gcctgcttca
    12121 aactttcgcc cctcctggca ttgattcagc ctgtcggaac tggtatttaa ccagactaat
    12181 tattttgatg cgcgaaatta atcgttacag gaaaagccaa agctgaatcg attttatgat
    12241 ttggttcaat tcttccttta gcggcataat gtttaatgac gtacgaaacg tcagcggtca
    12301 acacccgcca gcaatggact gtattgcgct cttcgtgcgt cgcgtctgtt aaaaactggc
    12361 gctaacaata caggctaaag tcgaaccgcc aggctagact ttagttccac aacactaaac
    12421 ctataagttg gggaaataca atgttccagc aagaagttac cattaccgct ccgaacggtc
    12481 tgcacacccg ccctgctgcc cagtttgtaa aagaagctaa gggcttcact tctgaaatta
    12541 ctgtgacttc caacggcaaa agcgccagcg cgaaaagcct gtttaaactg cagactctgg
    12601 gcctgactca aggtaccgtt gtgactatct ccgcagaagg cgaagacgag cagaaagcgg
    12661 ttgaacatct ggttaaactg atggcggaac tcgagtaatt tcccgggttc ttttaaaaat
    12721 cagtcacaag taaggtaggg ttatgatttc aggcatttta gcatccccgg gtatcgcttt
    12781 cggtaaagct ctgcttctga aagaagacga aattgtcatt gaccggaaaa aaatttctgc
    12841 cgaccaggtt gatcaggaag ttgaacgttt tctgagcggt cgtgccaagg catcagccca
    12901 gctggaaacg atcaaaacga aagctggtga aacgttcggt gaagaaaaag aagccatctt
    12961 tgaagggcat attatgctgc tcgaagatga ggagctggag caggaaatca tagccctgat
    13021 taaagataag cacatgacag ctgacgcagc tgctcatgaa gttatcgaag gtcaggcttc
    13081 tgccctggaa gagctggatg atgaatacct gaaagaacgt gcggctgacg tacgtgatat
    13141 cggtaagcgc ctgctgcgca acatcctggg cctgaagatt atcgacctga gcgccattca
    13201 ggatgaagtc attctggttg ccgctgacct gacgccgtcc gaaactgcac agctgaacct
    13261 gaagaaggtg ctgggtttca tcaccgacgc gggtggccgt acttcccaca cctctatcat
    13321 ggcgcgttct ctggaactgc ctgctatcgt gggtaccggt agcgtcacct ctcaggtgaa
    13381 aaatgacgac tatctgattc tggatgccgt aaataatcag gtttacgtca atccaaccaa
    13441 cgaagttatt gataaaatgc gcgctgttca ggagcaagtg gcttctgaaa aagcagagct
    13501 tgctaaactg aaagatctgc cagctattac gctggacggt caccaggtag aagtgtgcgc
    13561 taacattggt acggttcgtg acgttgaagg tgcagagcgt aacggcgctg aaggcgttgg
    13621 tctgtatcgt actgagtttc tgttcatgga ccgcgacgcg ctgcccactg aagaagaaca
    13681 gtttgctgct tacaaagcag tggctgaagc gtgtggctcg caggcggtta tcgttcgtac
    13741 catggacatc ggcggcgaca aagagctgcc atacatgaac ttcccgaaag aagagaaccc
    13801 gttcctcggc tggcgcgcta tccgtatcgc gatggatcgt aaagagatcc tgcgcgatca
    13861 gctccgcgct atcctgcgtg cctcagcttt cggtaaattg cgcattatgt tcccgatgat
    13921 catctctgtt gaagaagtac gtgcactgcg caaagagatc gaaatctaca aacaggaact
    13981 gcgcgacgaa ggtaaagcgt ttgacgagtc aattgaaatc ggcgtaatgg tggaaacacc
    14041 ggctgccgca acaattgcac gtcatttagc caaagaagtt gatttcttta gtatcggcac
    14101 caatgattta acgcagtaca ctctggcagt tgaccgtggt aatgatatga tttcacacct
    14161 ttaccagcca atgtcaccgt ccgtgctgaa cttgatcaag caagttattg atgcttctca
    14221 tgctgaaggc aaatggactg gcatgtgtgg tgagcttgct ggcgatgaac gtgctacact
    14281 tctgttgctg gggatgggtc tggacgaatt ctctatgagc gccatttcta tcccgcgcat
    14341 taagaagatt atccgtaaca cgaacttcga agatgcgaag gtgttagcag agcaggctct
    14401 tgctcaaccg acaacggacg agttaatgac gctggttaac aagttcattg aagaaaaaac
    14461 aatctgctaa tccacgagat gcggcccaat ttactgctta ggagaagatc atgggtttgt
    14521 tcgataaact gaaatctctg gtttccgacg acaagaagga taccggaact attgagatca
    14581 ttgctccgct ctctggcgag atcgtcaata tcgaagacgt gccggatgtc gtttttgcgg
    14641 aaaaaatcgt tggtgatggc attgctatca aaccaactgg aaacaaaatg gtcgcgcctg
    14701 tagacggcac cattggtaaa atctttgaaa ccaaccacgc gttctctatc gaatctgata
    14761 gcggcgttga actgttcgtc cacttcggta tcgacaccgt tgaactgaaa ggcgaaggct
    14821 tcaagcgtat tgctgaagaa ggtcagcgcg tgaaagttgg cgatactgtc attgaatttg
    14881 atctgccgct gctggaagag aaagccaagt ctaccctgac tccggttgtt atctccaaca
    14941 tggacgaaat caaagaactg atcaaactgt ccggtagcgt aaccgtgggt gaaaccccgg
    15001 ttatccgcat caagaagtaa ttcttgccgc agtgaaaaat ggcgcccatc ggcgccattt
    15061 tttatgcttc cgccagcggc ggcaaaatca attcatcgct ctcatgctgc tgggtgtagc
    15121 gcatcacttc cagtacgcgc aaccccgcac ggtgcaccgc atcgtttaac gccttccctt
    15181 tcagcaaacc actgataagc tgagcacaaa acaggtcgcc agtccctttc aggtcggttt
    15241 ttacccgtga atgggaaatg acattcacgc tgtcggcact gaccaccacc acctgcatct
    15301 cctgattttc ttcattaccg gaggcgctgg taatcaccac ccattttaat gtgtctgaaa
    15361 gcagactttt tgcggcagca atggcactgt cgagatcgcg gcaattttta ccggtcagga
    15421 tttccagctc aaagatattg ggggtaattc cctgcgccag cggcagtaaa tattgtcgat
    15481 acgcctcggg aaggtcaggt ttgacataaa ttccgctatc aatatcgcca atcactggat
    15541 cgaccatgat caataggtca ggatgatctt tgcgtagcgc agtcagccac tcggcaagga
    15601 ttttgatttg cgatgccgtt cccatatagc ccgtggttac agcacgaagt tggcgcaacg
    15661 catcacgctc ctgaagcgca cgcaaatagc cgctaaacca ttcgtccgga atcgcaccac
    15721 cgtagaaagt gtcataatgc ggtgtattgc tcagcaatac cgtcggcacg gcaaagacat
    15781 tcaggccgtt ctgtttgata gcaggcacgg caatgctgtt gcctacgctg ccgtacacta
    15841 cctgcgactg cacggcgacg atatctgcct gcaatgccct gctcttatcg ttaaacaaca
    15901 acaaactact catttaattt tttctccttg ccgatgatcc tcatcgtatt ccaaccgaaa
    15961 ctttacctga ttctggcagt caaatcggct atcacaaaac aaggataagg taattcaatg
    16021 aagaaaatca tttgtctggt cattacgctg ctaatgacac tccccgttta cgcgaagtta
    16081 actgcccatg aagaagcccg tatcaacgcc atgctggagg gattagcaca gaaaaaggat
    16141 ttgatatttg tgcgtaacgg cgatgaacac acctgtgatg aagcggtttc ccacctgcgt
    16201 ctgaagctcg gcaatacccg taaccgcatt gacactgccg aacaatttat tgataaggtt
    16261 gcttcgtcat catcgattac cgggaagccg tatatcgtga agatgcccgg taagagcgat
    16321 gaaaatgcgc aacctttttt acatgcgctg attgcgcaga cggataaaac ggtgtctgcg
    16381 caataatctt aaatccccgc cccctggcta aagtgttctt ccccaaacac gccggtggag
    16441 aggtagcgat cgccgcgatc gcagatgatc gccacaacca ccgcgccggg gttagctttt
    16501 gccacgcgca gtgctccggc aaccgcgccg ccggagctaa cgccacagaa tattccttcc
    16561 cgcaccgcca gttcacgcat ggtgttttct gcatcgcgct gatgaatatc cagcacctca
    16621 tccaccagcg aagcgttgaa aatccccggc agatattccg caggccagcg gcgaatgccg
    16681 ggaatgctgc tgccctcttc cggttgcagg ccgacaatgg tcaccggttt ggattgttcg
    16741 cgcataaagc gtgacacgcc ggtgatggtg ccggttgttc ccatgctgga gacaaaatgg
    16801 gtaatgcgcc cgccagtttg ctgccagatt tccggcccgg tggtggtgta atgcgcataa
    16861 gggttatcgg gattattgaa ctgatcgagc agctttcctt caccacgatt cgccatctcc
    16921 agcgccagat cgcgcgcacc ttccatgccc tgctctttgg tgacaagaat cagttccgca
    16981 ccataagcac gcatcgccgc acggcgttcc tggctcatgt tgtcgggcat cagcaatttc
    17041 atgcgatagc ctttaagcgc ggcaatcatt gccagcgcaa tgccggtgtt accactggtg
    17101 gcttcgatca gcacgtcacc cggtttaatt tccccgcgct tttccgcctc gacgatcatc
    17161 gaaagtgccg cacgatcttt caccgaacct gccgggttat tgccttccag tttcaaccag
    17221 acttcactgc cgttattcgg ccccattcgc tgcaacttca ccagaggcgt attgccaatt
    17281 gtttgttcta atgtactcac gatctctatc catacgtggt gttgcctgat gcgacgcttg
    17341 cgcgtcttat caggcctaca ggttacaaac cttgccatta aaaagcccgg atcgcgggaa
    17401 gcgcctccgg gcgtttaaca ttcactcaac ctatcaggcg ctttgtgcga gagcaagttc
    17461 ctcatcgcgg gtttcaatac gctcgtcgcc gttatacagc cgcgcatgtt gcagaccaac
    17521 gaataaacgc tcgccacgtt gcggggcgtc gtcgccgtgc atcacgaccg tcagcgcttc
    17581 gttgtaccac cccagcggct gcacgactaa ttgggtgtag tggcctttcg ggctggcttc
    17641 cagtacctgt accggcagcg gcgaatcgag gctggtacgg cggctgatat ccacttccca
    17701 cgggcgcagg aagagatcca ccggcccctg atacgcaggt gtatagccca gcggccagcg
    17761 atgtgcgcca acatggaact gcccgccgcg aatggttccc tgcaggcggt tcacttcgcc
    17821 cataaattcg agcacaaagc gggtcgctgg ttcgcgccat acctgatccg gcgcatcagc
    17881 ctgctcgata ttgccctggc tcatcaccac tacacgatcg gctacttcgg tcgcttcttc
    17941 ctggtcatgg gtcacgaata cgctggtaaa tttcagttct tcatgcagtt gacgcaacca
    18001 gcgacgcagc tctttacgca cctgcgcatc cagcgcgcca aacggttcat caagcagcag
    18061 aatttgcggt tccacagcga gcgcacgcgc cagcgccacg cgctgtttct gcccgccgga
    18121 aagctgtgcc ggataacgat ccgccagatg cgcaagctgc accatctcca gcaattttgt
    18181 cactttcgct ttgatggctg cggcattcgg gcgctcgcga cgcggcagca ccgtcaggcc
    18241 gaaagcgata ttgtcgaaca ccgtcatatg gcggaacagc gcatagtgct ggaacacgaa
    18301 accaacttta cgatcgcgcg catgcaggcg gctgacgtcg gtgccgtgga agcgaatatg
    18361 cccgctggtt tgatgctcca gcccggcgat aatgcgcagc agcgtggttt tcccggaacc
    18421 ggacggcccc agcaacgcga ccatctgacc agaaggaata tccagtgaga tatcgttcag
    18481 cacctgggtg cgaccaaaag acttcttaat attggcaatc tcaatgctca tgatgttcct
    18541 cctgctgcgc gcgtttttcc tgattctcca gacgccactg caacatactc tttaaaaaca
    18601 gggtgataat cgccatcagc gtcaacaacg cggcagcggt aaaggagccg acggtgttgt
    18661 agtcttgctc cagcaattca atctgtaacg gcagcgaaag tgtttcgccg cgaatcgagc
    18721 cggaaaccac cgacaccgca ccaaactcgc caatcgcgcg ggcgttggtc agcaccacgc
    18781 cataaagcag cgcccagcgg atgttcggca gcgtgacgcg gcgaaacatc tgccagccag
    18841 atgcgcccag caaaatcgcc gcttcgtctt cctggctgcc ctggcttaac atcaccggca
    18901 ccagctcacg caccacaaac ggacacgtca cgaagatggt gaccagcacc attcccggcc
    18961 aggagaacat aatttgcaga ttatgttcat ccagccagcc gccgagcggg ccgttagagc
    19021 cgtaaaacag cagatacacc agaccggcaa ccaccggcga cacggcaaac ggaatgtcca
    19081 gcagcgtcag cagcaactgg cgtccgggga agttaaagcg cgtcaccagc catgccagca
    19141 gaatgccgaa caccaggttc accggcacgg caatcagcgc aatcatcacc gtcagccaga
    19201 tggcgtgcag catgtccgga tcggccagat tctgtaaaac tggcatcagc cctttactga
    19261 acgcctgcac aaagatgtaa atcatcggca ccagcaggat gaacgccgaa accagcatcc
    19321 cgatgccaat cagaaaccat ttgccccagt taatcgggcg cgcgtcataa cgcttcaact
    19381 gtgtcacttc cgccattaat gacctaccac acgccgacca aagcgacttt gcagagtgtt
    19441 aattgagaac agcagcagca gggatgccgc gaggatcacc gaagcaatcg cgctcgccgc
    19501 cgggtaatca aactcctgta agcgcacaaa aatcatcagc gacgtcactt ccgtcttcca
    19561 ggcgatgttc ccggcgataa aaatcaccgc gccaaactca ccaagactac gagtaaacga
    19621 cagcgccacg cccgccacca gcgccggaga aagttccggc agcaccactt tgcagaaact
    19681 ctgcctgcgc gttgcgccaa gcgtttctgc cgcttcttca tattccgggc ctaactcttc
    19741 cagcaccggc tgcacggtac gtaccacaaa cggaatgctg gtaaaggcca tcgccaccgc
    19801 aatccccagc caggtatagg tgactttgat atcaaatttc gccagccatt caccgtaaaa
    19861 accgtttaca gaaaagagcg aggccagcgt taaaccggcg actgccgttg gcagcgcaaa
    19921 gggtaaatcc atcagcgcat caagcagcgt gcggcccgga aagcgataac gggttaagat
    19981 ccacgccatc agcaggccga aaacgccgtt aaaaattgat gccacaaacg ccgacagcag
    20041 cgtcactttg taggccgcga ccacttgtgg gttggtgatc acctcccagt actgcgccca
    20101 gctcatctga gccagctgca tcaccagcgc ggagagcggc agcagcaaaa taaggcacac
    20161 aaacagcaga ctggtgccga ggcttaaggt aaagcccggc agcacgcgtc ttgatgaaac
    20221 tgcaaacatt aattacgccc cgccgctaac agcttgtcta actcgccacc gctggtgaaa
    20281 tgggttttca tcacttccgg ccaggagcca aatttgtctt ccacgcggaa cagctcggtc
    20341 tgcgggaatt tatctttcag tttgtccatg acttccgggt tatttacgcg gtagtaatag
    20401 tcggtgatga tggtctgcgc ctgcgggctg tagagccagt tcaggtaagc ttttgctgct
    20461 ttttccgtac cgttggcctg cacgttttta tcgacccacg ccaccgggaa ttccgccaga
    20521 atattggttt tcggaatcac cacttcaaag ccttgcgctt catactgttt gcgaatgttg
    20581 ttcacttccg actcgaagct gatcagcaca tcgcccaggc cgcgctcggc aaaagtggtg
    20641 gtcgcgccac gaccgccagt atcgaacact tcaacgtttt tcaggaactg ggtcataaac
    20701 tgttcggttt tggctttgtc gccaccgtca gctttatccg ctgcgcccca tgccgccaga
    20761 taggtgtaac gtgcgttacc cgacgttttc gggttcggga aaatcagctt tacgtcggag
    20821 cgcaccagat cgttccagtc gtggatattc ttcgggttac ccttacgcac caggaagccc
    20881 atggtggagt agaacggcga gctgttattc ggcaggcgcg tttgccagtc ggccgggatc
    20941 agcttgcctt tgtcgtgcag gatttgtacg tcagtcacct ggttataagt gacaacgtcg
    21001 gcttttaagc cctgcaaaat cgccagcgcc tgttttgatg acccggcatg agattgtttt
    21061 atcgtcagtt tgtcgccgcc gttatctttg gcccattgtt gctcaaacgg cggattcagg
    21121 gcggcaaaca gctcgcggga gacgtcataa gaactgttca gcagttccgt tgcctgtaca
    21181 tggcccgcca gcagcagaga agcgaccagc gcgagtgagt tctttttcag taagttaacg
    21241 gccattgcgc acccttataa atttaatgac tttctaatag ccatcatatt tataacggac
    21301 gtgaaaggag taacggtttt atataccgtt tggtgatttg gaagttgaaa agggaataag
    21361 aaccgggttc aagacgaaat cctccccaca aatgcaggga ggaaacagag gtgaatcaga
    21421 tgccgacgct aaccgtctcc ggcagtgtgc tgccgccatc aatcacattc tgtgtaccgg
    21481 ttaaataact tgattcatcc gatgcgagga aggctgccag ttcgccgact tccagcggat
    21541 cggcgaggcg acgcatcggg attgcttttg ccatttcagt cagcaccgat tctggatctt
    21601 ccgggttcga ctggcgggca atgctttccg ccattggcgt gcgtacgtat cccgggcaga
    21661 tggcgttaac gcgaatacca gactgcgcgt actccaccgc cagcgattta gtcaggccaa
    21721 caatcgccgc tttcgttaag gcatacgccg tttcacccgg atcggccacc atatcgccag
    21781 tgactgaaga catcatcaca atgcgaccat ctttgcgggc aatcatctcc ggcagcaccg
    21841 ccttcgtgac gttccatacg cctttaatat tgatatcaat atggaaatcg cgatcttcat
    21901 cgctcatatc gaggaaactg cccagacgac aaacgcctgc gttattcacc aggatatcaa
    21961 tgcgcccttc tttttccttc gcgcgcttga tagctgcggc gaccgacgcc gggtcacgca
    22021 catcggcgac aaccgccgta cagcgatgac cacgaccaca cagttcgtcc gccagctttt
    22081 cgatctcagg ggagatatcc agcaagatta ggtttgcgcc atgacgcgca aaagttctgg
    22141 caattccttc gccaattccc tgcaatgcgc ccgtaatcag tgctgtcttg cccgtgagtt
    22201 tacccatttt aatgctctcc ttgttaatac agagcgttac actccattcc ctctgaaaat
    22261 acaggataaa caaaatattg ctgcggatca gctcactaat tcaggaaaat ctcaatccag
    22321 tcgctgcttt atgcatcatt tcaggcgttg cgttagctca ctgctgcgct caatcatttt
    22381 caatgactcc acatcattga gttgcacaag gccaacaaac agtaaatcgg taacagaatt
    22441 ttgcgccgtg cgggtggaca tcgacgagct gcgccattcg gtttcgccag aaacggtatc
    22501 gagggtgtaa tgcgccagcc gccgcagcgg ggaatcggtt aacgaggtaa tggcaatcac
    22561 cgttgccccc tgctttcgtg ctgcctcggc gcacaagaca atctcttttt tactgccgct
    22621 gtaagagata gcgatttgta catcgccttt tttcagcgcc tgggaaacgg tggcttgtac
    22681 atgcgtgtca gcttcacagg ccacacgata accaattttc atcagtttga atgaaagatc
    22741 gcgccccacc agcgccgacc cacccaggcc cgttatctgg ataaacggtg ctttactgat
    22801 gacctcaatg attttttgca accgcgcgta gtcgaacaac gcacaggttt gctccagcgc
    22861 cagctctttt tcgcgattca atttgcgggc aatcacttcc agcgaatcat cactggtaat
    22921 cgaactgtgc aggtgcagcg ccgtggcatt cgttttctcc cggctagcac tgtattcgcc
    22981 aattaacgcc atacgcaatt cagtaaatcc ttgcgcaccg agcttctggg caaacttcac
    23041 aatactcgac tggctgatgc cgagctgttt cgccatcttg cgcgaagaga cggatttcag
    23101 ctcactcacg ttggcccgca gaaagtcagc aattttttgc tcgttcccgg taaattcact
    23161 ttccgcattg cgtattttcg tcaggtacaa catcttccac tctcctgggc tggcagcaaa
    23221 aattccgctt aagttcttat gacgctcttc acactctgcg agtgtagact caatgattcc
    23281 tttaagtctg tcaattcggg atatatgatt ccatacatct gcaattattt gatcgtaaat
    23341 agtaaggtca ccaccgatgc aacttgaaaa gatgattacc gaaggctcga acgccgcctc
    23401 ggctgaaatt gaccgcgtat caacgctgga aatgtgccgg attatcaacg atgaagataa
    23461 aaccgtacca ctcgccgttg agcgcgtact gccggatatc gccgcggcga tcgatgttat
    23521 ccacgcccag gtaagcggcg gcgggcgtct gatatatctc ggtgcgggaa catccggtcg
    23581 tctggggatt ctggatgcca gcgaatgtcc gcccacctac ggcgttaaac cgggtctggt
    23641 ggttggtttg attgctggag gcgaatatgc cattcagcac gcggtggaag gcgctgaaga
    23701 tagccgggaa ggtggtgtta acgatctgaa aaacatcggc ttaaccgcgc aggatgtggt
    23761 ggttggcatt gcggccagtg gtcgcacgcc gtatgtaatt gccggactgg aatacgcacg
    23821 ccagctcggc tgccgcacag tgggaatttc ctgtaatccc gggagcgccg tttcaaccac
    23881 cgctgagttt gccattacgc cagttgttgg ggccgaagtg gtcacaggtt cttcgcgaat
    23941 gaaagcaggt acggcgcaga aactggtgct caatatgctc tccaccgggc tgatgattaa
    24001 atccggcaaa gtgttcggca acctgatggt cgatgtggtc gccaccaacg aaaaattgca
    24061 tgtgcgccag gtcaatatcg tcaaaaacgc taccggatgt agcgcagagc aagcggaagc
    24121 ggcattggtt gcctgcgagc gcaactgtaa aacggccatt gtgatggtgc tgaaaaatct
    24181 cgatgccgca gaagctaaaa aacgtcttga tcaacacggc ggctttattc gtcaggtttt
    24241 agacaaggaa taacccatgg ccaaagagat cagcagtgaa cttctgaaca ccattcttac
    24301 ccgtgtcggc ggaccgggaa atatcgccag ttgtggtaac tgtatgacgc gcctgcgtct
    24361 gggtgtacat gacagttcac tggttgatcc caatatcaaa acgctggaag gcgtgaaggg
    24421 cgtcattttg accagcgatc aggtgcaggt ggtttttggg ccgggcaaag cgcatcgtgc
    24481 cgcaaaagcc atgagcgagc tgctgggaga agcaccggta caggatgccg cagaaatcgc
    24541 cgcccagaac aaacgtcagt taaaagccaa acaaacctcc ggcgtgcaac aatttctcgc
    24601 caaattcgcc accatcttca cgccgctgat ccccggtttt attgccgccg gtctgctgct
    24661 ggggatagcg acgttaattg ccacggtgat gcacgttccg gcagatgctc agggcacgct
    24721 acccgacgcg ctgaatttta tgaaggtgtt cagcaaaggt ttgttcacct tcctggtgat
    24781 tctggtgggc tataacgccg cccaggcatt tggcggcacg ggcgtaaatg gcgcgattat
    24841 cgccgcgctc tttttgctcg gttataaccc aactgcaacc accggttact acgccggttt
    24901 tcacgatttc tttggtctgc ccatcgatcc gcgcggcaat attatcggcg tgttgattgc
    24961 cgcgtgggcc tgcgcccgca tcgaaggcat ggtgcgccgc tttatgccgg acgatctcga
    25021 catgctgttg acctcgttaa tcaccctgct gatcaccgct acgctggcct acctgatcat
    25081 tatgccgctg ggcggctggc tgtttgaagg catgtcgtgg ctgtttatgc acctgaacag
    25141 taatccgctc ggttgtgcgg ttttagccgg gctgttcctg atcgccgtgg tgtttggcgt
    25201 gcatcagggc tttattcctg tttacctcgc gttaatggac agtcagggat tcaacagctt
    25261 atttcccatc ctttcgatgg caggcgcggg ccaggtgggc gcggcgctgg cactctactg
    25321 gcgggcgcaa ccgcacagtg cattacgcag tcaggtacgc ggggcgatta ttcccggcct
    25381 gctgggcgtt ggcgaaccgt tgatttatgg cgtcaccctg ccccgcatga aaccgtttat
    25441 taccgcctgt ttaggcggcg cggcgggcgg tttgtttatc ggattgatag cctggtgggg
    25501 tctgccgatg ggcttaaaca gcgcctttgg gccgtctggt ctggtggcgc tgccgctgat
    25561 gacttccgca caaggcatcc ttccggcaat ggcggtttat gcaggcggga ttctggtggc
    25621 atgggtttgc gggtttattt tcaccacgct ctttggctgc cgtaacgtca atctggactg
    25681 ataatatgaa acggacaatg ctctatcttt ctctgctggc tgtcagctgt agcgtcagcg
    25741 ccgcaaaata ccctgttctg acagaaagtt cgccagagaa agcagggttc aacgtcgaac
    25801 ggcttaacca gatggatcgc tggataagcc agcaggtgga tgccggttat cccggcgtaa
    25861 acctgctgat tattaaagat aatcagattg tgtatcgcaa agcctgggga gcggcgaaaa
    25921 agtacgatgg cagcgtattg atggaacagc ctgtcaaagc caccaccggg acgctgtatg
    25981 acctggcttc caataccaag atgtacgcca ctaactttgc tctgcaaaag ctaatgagcg
    26041 aaggcaagct gcatcctgac gatcggattg cgaaatatat tccgggattt gccgatagtc
    26101 caaatgacac catcaaaggc aaaaacacgc tgcggatttc tgacctgctg catcacagcg
    26161 gcggtttccc ggcggaccca caatatccga ataaagcggt cgcgggcgcg ttatattccc
    26221 aggataaagg ccagacgctg gagatgatca agcgcacgcc gctggagtat cagcccggca
    26281 gcaagcatat ctacagcgat gtcgattata tgctgcttgg atttatcgtc gagtcggtta
    26341 ccggtcagcc acttgaccgc tatgttgaag agtcgattta tcgcccgctc ggcctgacgc
    26401 atacggtgtt taatccgcta ctgaaaggct ttaaaccgca acaaattgcc gccacggaac
    26461 tcaacggcaa tacccgcgat ggcgtgatcc atttcccgaa tatccgcacc tccactctct
    26521 ggggtcaggt gcacgatgaa aaagcctttt attcgatggg cggcgtttcc gggcatgcag
    26581 ggttgttttc caataccggc gatattgcgg tgttaatgca aacgatgctg aacggcggcg
    26641 gctatggcga tgtgcagttg ttcaatgcag aaacggtgaa gatgttcacc accagctcta
    26701 aggaagatgc cacttttggc cttggctggc gcgtgaatgg taatgccacc atgacgccga
    26761 cgtttggcac gctggcaagc ccgcaaacct acggtcacac tggctggacc gggacggtga
    26821 ccgttatcga tccggtgaat catatggcga ttgtgatgtt aagcaacaag ccacattcgc
    26881 cagttgccga tccacaaaaa aatcccaata tgttcgaaag cggtcagcta ccgattgcaa
    26941 cttatggttg ggtagttgat caggtgtatg cggcgttaaa gcagtaataa aaaggccagt
    27001 cagttgtgat actggctggc cttaaatcat cgaatcagaa acgattacaa cgccatcaac
    27061 ttgtccagcg acggcgcgaa gtaatagccg ccagtgaccg gtttggtgaa acgcaacatc
    27121 gcatcacgtt tgccatcggt atcgccaaac atgctcagca gttgctgctc aatgttatgc
    27181 agacgcgcgc agtaggcgca gaagtacaga ccgtgagtgc cactggcggt gccgtacggc
    27241 aggctctggc gaacaatctt cagccctttg ccatcttctt tcagatcaac gcgggtcagg
    27301 tgagaggttt ccggacgttc gtcgccgtca atctcttcgt tggcctcttt agtgcgcccg
    27361 atcatcatct cttgatcgtg aacgctcata cggttgagtt gcttcaggtt gtgctcccaa
    27421 cgctgcacaa acacatagct gccgcccgca tccacgccgt ctttgataac cgccacttcg
    27481 cggcgcgtct cttcacccgc cgggttttct gtaccgtcaa cgaagccgct caggtcacgc
    27541 tcttcaaccc aacggaagcc gtggatctct tctttcactt caatgcagtc accaaaggct
    27601 tccatcgccg cctgagcgac agagaagttt acgtcgtgac gcagagaaag aatgtggatc
    27661 aacacatcga actgggtcgt cggcgcaagg cctttaccgt agcccggaaa atctttcagc
    27721 tcttcggccc caacgccgcc gctcagagcg cgccaggtgt tgttaccaaa ggcaaccacc
    27781 gcaccaagat gcgcatccgg gaatttcgct tcaaaagttg ccagtttatc ggcaaatgtt
    27841 ttactggccg cacgcagggc gtcaacttcc cctttcacgt tggcttcgat ccaaatcgcc
    27901 gcgcggcaat gttctggcaa aatgccactc tgaacctgag acattgttcc tcctga
//
LOCUS       DACBVI010000049        27907 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.049, whole genome shotgun sequence.
ACCESSION   DACBVI010000049 DACBVI010000000
VERSION     DACBVI010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.049"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..577)
                     /gene="paaY"
                     /locus_tag="IU325_10325"
     CDS             complement(<1..577)
                     /gene="paaY"
                     /locus_tag="IU325_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HAP5809417.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPDQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPGRKRLVFDENL"
     gene            complement(559..1509)
                     /gene="paaX"
                     /locus_tag="IU325_10330"
     CDS             complement(559..1509)
                     /gene="paaX"
                     /locus_tag="IU325_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HAP5809418.1"
                     /translation="MSKLDTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKATLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEALCQFTR"
     gene            complement(1610..2923)
                     /gene="paaF"
                     /locus_tag="IU325_10335"
     CDS             complement(1610..2923)
                     /gene="paaF"
                     /locus_tag="IU325_10335"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HAP5809419.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGSPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPQDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(2950..4155)
                     /gene="pcaF"
                     /locus_tag="IU325_10340"
     CDS             complement(2950..4155)
                     /gene="pcaF"
                     /locus_tag="IU325_10340"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HAP5809420.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(4155..4577)
                     /gene="paaI"
                     /locus_tag="IU325_10345"
     CDS             complement(4155..4577)
                     /gene="paaI"
                     /locus_tag="IU325_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HAP5809421.1"
                     /translation="MSNKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(4570..5994)
                     /gene="paaC"
                     /locus_tag="IU325_10350"
     CDS             complement(4570..5994)
                     /gene="paaC"
                     /locus_tag="IU325_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaC"
                     /protein_id="HAP5809422.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAADLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYE"
     gene            complement(5996..6784)
                     /gene="paaB"
                     /locus_tag="IU325_10355"
     CDS             complement(5996..6784)
                     /gene="paaB"
                     /locus_tag="IU325_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase"
                     /protein_id="HAP5809423.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLITGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(6784..7551)
                     /locus_tag="IU325_10360"
     CDS             complement(6784..7551)
                     /locus_tag="IU325_10360"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase"
                     /protein_id="HAP5809424.1"
                     /translation="MSELIVSRQQRVLLLTLNRPAARNALNNVLLTQLVNELEAAAID
                     PSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(7548..8618)
                     /gene="paaK"
                     /locus_tag="IU325_10365"
     CDS             complement(7548..8618)
                     /gene="paaK"
                     /locus_tag="IU325_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HAP5809425.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASLNGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDDAEAALKALGMPEKAIHLERFNTAGTRVKRNVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(8626..9123)
                     /gene="paaD"
                     /locus_tag="IU325_10370"
     CDS             complement(8626..9123)
                     /gene="paaD"
                     /locus_tag="IU325_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate degradation protein PaaD"
                     /protein_id="HAP5809426.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTNGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(9138..9884)
                     /gene="paaC"
                     /locus_tag="IU325_10375"
     CDS             complement(9138..9884)
                     /gene="paaC"
                     /locus_tag="IU325_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HAP5809427.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESREPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAIN
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGFNEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMVAEMQYLQRVLPGQQW"
     gene            complement(9893..10180)
                     /locus_tag="IU325_10380"
     CDS             complement(9893..10180)
                     /locus_tag="IU325_10380"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HAP5809428.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(10192..11157)
                     /gene="paaA"
                     /locus_tag="IU325_10385"
     CDS             complement(10192..11157)
                     /gene="paaA"
                     /locus_tag="IU325_10385"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HAP5809429.1"
                     /translation="MNHENWNRKGDDVTQEERFEQRIAQETAIEPQDWMPDAYRKTLI
                     RQIGQHAHSEIVGMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAR
                     EDIYQKMLDGRMKYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAM
                     VKICKEESFHQRQGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSA
                     RSLAWKIKRFTNDELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEF
                     NEVINGRGICNQERLDAKRKAWEEGTWVREAALAHAQKQHACKVA"
     gene            11406..13451
                     /gene="paaZ"
                     /locus_tag="IU325_10390"
     CDS             11406..13451
                     /gene="paaZ"
                     /locus_tag="IU325_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HAP5809430.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGGRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSATTGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQVLVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNTLLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQQHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLENLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWSVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            13699..15972
                     /gene="tynA"
                     /locus_tag="IU325_10395"
     CDS             13699..15972
                     /gene="tynA"
                     /locus_tag="IU325_10395"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HAP5809431.1"
                     /translation="MGSPSLYSARKTALALAVALSFAWQAPVFAHGGEAHMVPMDKTL
                     KEFGADVQWDDYAQIFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDTKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSSHDTGLGQYSKDNESLDNTDAVIWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGAIKKDK"
     gene            complement(16031..17530)
                     /locus_tag="IU325_10400"
     CDS             complement(16031..17530)
                     /locus_tag="IU325_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAP5809432.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTVDANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHGEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGSVCGAALTSHPHVAKISFTGSTATGKGIARTAADRLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVRSFLDDAQAQQAELIRGASGPAGEGYYVAPTLVVN
                     PNAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            17766..18671
                     /gene="feaR"
                     /locus_tag="IU325_10405"
     CDS             17766..18671
                     /gene="feaR"
                     /locus_tag="IU325_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HAP5809433.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGRLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(18843..19172)
                     /locus_tag="IU325_10410"
     CDS             complement(18843..19172)
                     /locus_tag="IU325_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HAP5809434.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVNDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(19177..19362)
                     /locus_tag="IU325_10415"
     CDS             complement(19177..19362)
                     /locus_tag="IU325_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HAP5809435.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(19359..21998)
                     /locus_tag="IU325_10420"
     CDS             complement(19359..21998)
                     /locus_tag="IU325_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HAP5809436.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVEFDSACLAKL
                     PQTEQSLAAPKTLAQWQAMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLSQ
                     EPSLVDAELDWQENSGQLIVVARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVVMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAISGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTNINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            22206..23195
                     /gene="ldhA"
                     /locus_tag="IU325_10425"
     CDS             22206..23195
                     /gene="ldhA"
                     /locus_tag="IU325_10425"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAP5809437.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            23306..23728
                     /gene="hslJ"
                     /locus_tag="IU325_10430"
     CDS             23306..23728
                     /gene="hslJ"
                     /locus_tag="IU325_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HAP5809438.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGKLTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(23725..23991)
                     /locus_tag="IU325_10435"
     CDS             complement(23725..23991)
                     /locus_tag="IU325_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HAP5809439.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            24265..27789
                     /gene="nifJ"
                     /locus_tag="IU325_10440"
     CDS             24265..27789
                     /gene="nifJ"
                     /locus_tag="IU325_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP5809440.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILELIPQAEIDAHRARGLNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMVERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKTARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDTNGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEIRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         6807 a         7522 c         6849 g         6729 t
ORIGIN      
        1 gcagattctc atcaaatacc aggcgtttcc tgccaggttc aacttcccgc aatggctcga
       61 cttgatgtaa cgtctgctta cagcgtgtca ccagcacctg atactcatgc gtgccctgct
      121 ttttccatgc cagttcctgc tcactaagtt cacgaatcgc ttttgccggg ctgccgacaa
      181 tcaggtaatt agcaggcatt tctgctttgg ctttcacaaa tgcggaagcg ccaacaatgc
      241 tgttctcgcc aatcaccgca ccgtccatca ctaccgcgtt catacccact aatgcattgc
      301 ggcggataat gcagccgtga aggatagcgc tatgaccaat atgcccatct tcttccacga
      361 cagtatcctg atcgggaaaa ccgtgcataa cgcaattatc ctgaatgttc gcgccatctt
      421 tcaccacgat acgaccgaaa tcgccacgca ggctggcatt tggcccaacg tacacgccct
      481 tgccgagaat aacatcgccg atcaataccg ctgtcggatg aacaaaactc tcttctggta
      541 caaccggagt cagaccgtct atctggtaaa ttggcataac gcctcctgtt caatgttcaa
      601 accgccaaaa cgttgaaaat acaggcttcc cggcgcaggc agttcaccga ccgaggtttc
      661 acctttttca ctaacgaacg ctaaagcagc aggcgctacg cgctgataaa tgttgataca
      721 cagctgacgc gccgtatgcc ctgcccagtg agccggaagc aactcctccg gcaacaatgg
      781 gtctttaagg acgacacggc gataaaaatg gatcagtaaa agctgaatat gaaatgcacg
      841 ctccggggtt aattcgtctg ccgcctcttt taacagcggc accagcgggc ggaatgactg
      901 aataaaggtt tcgtacatgg cattttgttc agttaaatgc cagcactctt ctactctggc
      961 acgcagtgct gcgcgagaaa gcgccagtgg tatttgcgct tcaaaacaaa tcacgttatc
     1021 cgccacaccc gcttcatgca aaagtgtctg tacatcggcc agtttttgcg acggcgatgc
     1081 catcagactg ggtgccagtg cgccaaaacc ttgccagatc aactgctttt tgacatcagc
     1141 cagcgtcgct ttatctaaac cttccgagag caacaggagc catttaccat cccatgcagg
     1201 ttgctctgcg cgataaattt tactttctgc ccgtcgcgtc aggcgcaagc ctttatcact
     1261 gaggctatag aaactgcgtc gcccgatgcg ggaaacatcc agccagcctt ctttattaag
     1321 acgaaacaaa gcggtgcgga cgaaacgctc accaaatccc agcccttcca gcaaagcagc
     1381 cagactaccc agccagattt caccaccacg atgggaaagc gaatcaccat acagagagga
     1441 gatcaaagat gtgccactga caggaacagc gtttacagca tgttggataa aagtatcaag
     1501 tttactcatt cgattcagtc tgttgttatt gttgtgattt atgaatcata gcacaggtcg
     1561 tatcgacttc tggccctggc gctttacctc accagggcca gacgacacat caggcaccaa
     1621 caatattgcg cagatcaaac acccgacacg ccttgccttc tgaacgcggg atactgccac
     1681 agttcacgat cattacatca gtagagatcc ccaccatcga cttaatccga tggcgtagct
     1741 ggtggcacac ctggcaacgc tgctcatgcg tcaatgtcag gctgctttct ttcaactcca
     1801 ctttcacaga aagtgaatca agatgccccc ggcggttcac ctccagttgg taatgcggcg
     1861 ataaatgttc gaatttgaca atctcttctt ccagttgtga cggaaagaca tttacaccgc
     1921 gaataatcaa catgtcatcg ctgcgtccgc tgatgcgatc catgcggcgc atagtccgcg
     1981 ccgttcctgg taacagtcgg gttaaatcac gcgtgcggta acgaatgacc ggcaatgctt
     2041 ctttggtcag cgtggtgaat aacagttcgc catgttcacc atcggcaagc ggtgtgccgt
     2101 cctgaggatt aacaatttca ggatagaaat gatcttccca aatggttggg ccgtcggtag
     2161 tttccagaca ctccatcgcc acccccggcc ccatcacttc tgacaggcca taaatatcca
     2221 gtgcggtgat ccccaggcga cgctcaattt ctttacgcat ggcctgtgtc cacggctcag
     2281 caccaaatac tccaacccgc agcgagcaac cgctggcatc accgcccaac tgccgctcca
     2341 gctcttcaat caggttaagg caataagatg gcgtaaccat gatcatatct ggttgaaaat
     2401 cacggatcag ttgcgcttgt ttttccgtct gcccgccaga catcgggatc accgtagcgc
     2461 ccagacgttc ggcaccatag tgcgcaccca gcccgccagt aaacaggccg taaccatagg
     2521 cgacatgaat tttgtctttg ggcgagcccc ctgccgcacg caaagaacgc gctacaatat
     2581 tggcccagtt atcaatatcg ttttgcgtat agccgacaac tgtcggttta cctgtggttc
     2641 ccgaagaagc atgaatgcgc accacttgtt ccattggcac ggcaaaggtg tcaaagggat
     2701 agttgtcgcg cagatcctgt ttggtggtac agggaaattt acgcaggtct gaaagttccc
     2761 tgaaatcatc aggatgtacg cctgctgcgt cgaatttgcg ccgatacatc ggcacatttt
     2821 catacgcgtg tttgagcgtc catttcagac gctgtgtttg caacgcctgt aactcatcaa
     2881 cagacgcggt ttcaatcggg tcaagctttg tattggttat cattgtaggt tactcgcagg
     2941 ttgatatgct caaacacgct ccagaatcat ggcgatgccc tgaccgacac cgatgcacat
     3001 ggtgcataat gcataacgac cgttacgccg atgcagctca tggctggcag ccagtgccag
     3061 gcgggcacca ctcattccca atggatggcc taaggcgata gcgcctccgt tggggttaac
     3121 atgtggggca tcatcaggca gccccaattc gcgtagtaca cccaacgcct gggccgcgaa
     3181 cgcttcgttc agttcaatca cgtccatatc gtgaatactc aaccccgcgc gttccagcac
     3241 ccgacgagtt gcaggcaccg gaccaagccc catcaggcgc ggttccaccc cggcggttgc
     3301 catggctacg atacgcgctc gcggtgtcag tccttgcgct gctgccatct gttcactggc
     3361 aataatcaac gcagcggctc catcattcac cccggaggca ttgcctgcgg tgatcactcc
     3421 attggcacga aatggtgctt ttagtccacg taactgttcc agcgtcgttt ccgggcgcag
     3481 atgctcgtca tgttgtattt ctgttacaac accttttttg tttttcaaca caaccggaac
     3541 aatctcttca gctaaaatgc ctgaagattg cgcttttgct gtacgttgct gactgcgtag
     3601 cgcaaaacta tcttgatctt ctcgcgagat ttttaacagt tcagctacat tctctgccgt
     3661 ttccggcatg ctgtcagttc caaattgttg agccatgagc gggttcacaa atcgccagcc
     3721 aatagtggta tcaaacatct cagcctgacg agaaaatgca ctggttgcct tgcccataac
     3781 aaacggtgcc cgagacattg actccacgcc accagcgatc agcaaatcgc catcgcccgc
     3841 tttaatcgcc cgtgcggcga accccagtgc gtccagtccg gaaccacaca agcggttaat
     3901 ggttgtgccg gaaacactct gcggcagccc cgccagtaaa gtcgccatcc gggctacgtt
     3961 acggttatct tctcccgcct gattagcaca gccgaggatc acatcatcga tacattccgc
     4021 atcgagacgc gggtttcgca ccagcagttc ccgcaaaggg atagcagcca gatcatcagc
     4081 ccgaacacct gataatgccc cgccgtagcg accaattggc gtacgaattc cgtcacagat
     4141 aaaggcttca cgcatcaggc ttctcctgta atggtgccgc cgatgcggtg agatttaccg
     4201 cgaaacagcg caaccgtttt ttgttgttgg ttaacaattt cgatgtcgta aacaccggtt
     4261 tgcttgccct gatgacgtac ctgcgcagta gcagttaagg tgtctccggc aaagcctgga
     4321 cgcaaaaaat caatcgtgca ggcagaagcg acggctgcca gcccctggct attgcaggcg
     4381 taggcaaagg cagtatcagc cagtgaaaat agctgcccgc cgtggcaact ttgatgacca
     4441 ttaagcattt gtgcagtgac ggtcatggtc actacagcaa agccttcatc cattgagata
     4501 atgtcgatgc caagcgcttt ggcgcaggca tcgttctcat acattgcatg ggcattttgc
     4561 caggccttat tactcataac cgctctccag aagcgctcgt tggcgcagca atgaacatgg
     4621 gcgatagcgt tcttcgccat agtgatgttg tagattttcc aggagcctta atattcgctg
     4681 ccagccaagt tgcgctcccc aggcaagcgg gccatatgga taattcaccc caagacgcat
     4741 ggcggtatcg atatcctgtt cagaggccac gcctttttga agcgcatcaa gggcttcatt
     4801 gatgatcatt gctaccgttc gccaaatcag cattcctggg taatctgcaa tttgcagcac
     4861 tgttttgccc tgctgttgca ggtaataaat ggccttgcgg gtcgctgagt ccgggttcac
     4921 cgctgccgca gcaatggtca ccaccttgcc cgccattttg tcgaccacta ccaccgggcg
     4981 tgccagtcgt attgccagcg cctgtgccgt ctcgccttgt gtctcaatca ataaaacatc
     5041 gtcaatttcc gtgacaccgt cacttttctt ttctactttc atggggctaa aactgtcgct
     5101 taccgcttcc aggccaacaa ctgcctcacg ttccgcgcgc cagtcgtaca cgcccagccc
     5161 acttttcttg cccaaccgtc caccaatcac cagttcctgt tgcaccagcg aaggtaaaaa
     5221 acgacgctcc tgccagaaag cgttaaacac cgaacaggtg acagcaaaat tgacgtcctg
     5281 accaatcaga tcggttaatt ccagcggccc catcgggaaa ccagcgccat cgcgaagtgc
     5341 agcgtcaatc acttctggtg cagcaacctg ctcttccagt gcccgccagg cctcggaata
     5401 ataaggacgc gcaacacggt taacgataaa tccaggagtc gaatgacagc gcacaggctg
     5461 cttaccccaa ctcaacgtta gttcacacaa ctgctcaaca acttccgctg ccgttgccag
     5521 cccactgacc acctccacca acttcatcac cggtgccggg ttaaaaaaat gcagccccgc
     5581 aacacgttca ggatttttta cctccgcagc aatcgcggtt atagagattg acgaagtgtt
     5641 agtggtcaat agcgtttgtg gcgggcaaac ttccgccagc tgtgcaaaga gcgctttttt
     5701 gacttccaga cgttcagacg ccgcttcaat gaccaggtcc gcagctgcca gcgcgtgaat
     5761 atcggtcacc gggatcaggc gtttcaatgt gcgttcacag gtttcagcag tcagttttcc
     5821 ccgcgtcact cgtgaattta gccgcgcgtg tatcccgtcg attgcgcggg tcagcgcttc
     5881 agcagaaatg tcatacagta aaacctgatg tccatgactg gcggcaactt cagcaatgcc
     5941 agcccccatg gtgccgctcc caatcactgc cacagtttgc acatttatca tcatgctatt
     6001 tccccgtgaa ctgcggtgag cgtttagcca ggaacgcact gacaccttca cgataatctg
     6061 cgctccgtcc ggcaagtcgc tgatagtcac gttccagatc cagttgcgta tcaagcgtat
     6121 tggtttcagc gctatttatc gcttgcttga taagtccaag accaaatgtc ggttgtgtcg
     6181 ccagatgccg tgccagctgt tgcgcggtat ctgccagcgt ttcatcatca acaacctgcc
     6241 agatcatccc ccattcgtgc gcctgttcag cactcagttg attccccagc agtgccagcc
     6301 ccatggcgcg cgctcgtccg gcaacgcgtg gcagtaacca ggtaccaccg caatcgggta
     6361 ccaaacctaa cttactaaac gccatgacga attttgctga acgggcagca ataacgatat
     6421 cgcaccccag tgccagtgtt gcgcctgccc ctgcagccac gccattaact gcacagatca
     6481 ccggttttgg cagttttgcc aggcgacgta ccagtgggtt atagaaacgc tccactgaca
     6541 tacctaaatc cggtgcgggg cctgtgggat caacgttacg atcgttaaga tcctgaccag
     6601 cacaaaatcc acgtccggca cctgtaatta acaagcaacg gatagtgtcg tcgcgctcga
     6661 cctgtttcag gcactctgcc agttgtgcgt gcatctcatc attaaaactg ttcaggcgtt
     6721 ccgggcggtt gagcgttagt gtcatcacgc ccttttctac atgactgagg atgaattcca
     6781 tcattagcgt cctttaaagt cgggcgtgcg tttttgtaag aaagcggaga tgccttcatg
     6841 acgatcttct gttgccgcca gcaaggtgaa taactgtcgc tcctgggcaa gtccggcttg
     6901 caaagccact tcctgcgact ggcgcagcgc ttgctttgcc gcttgtaagg ccagcggcga
     6961 gtgacgtgcc attttcgacg ccagctgtaa ggcgtattcg agggttaaat cgctggggaa
     7021 gacgtcgcta accagcccgg cctgctgtgc ttgctgagcg gtgatacttt ctccgctcag
     7081 caccattttg ctggctaacg atttaccgac actacggatt aaacgttgcg ttccgcctgc
     7141 gccaggcatg atgccgagag tgatttccgg caacccaaaa cgcgcgttct ctccggcaac
     7201 caccacatcg cacaacaatg ccagttcgca acccgcacca agcgcgtaac cgttgacggc
     7261 tgcgatgagt ggtttgttaa aggcctgcaa tcgcgcccat agctgcggac gtgtatcgtt
     7321 taaggtggcc gcgagatctt tttctgccat ttcgttgaga tcggccccag cggcaaaaaa
     7381 gcgtgcatta ccggtaatca cacagaccga aatgctggga tcgatagccg cagcttccag
     7441 ctcatttacc agttgcgtca gcaggacatt attcagcgca ttacgtgcgg caggacggtt
     7501 aagggtcagc aacaatactc gttgctgacg gctgacgatc agttcgctca tgccatcccc
     7561 ttcgcgtcaa agtcaaccac cacatcgctg gtcagcggca gtgcctggca actcaacaca
     7621 tagcctgcag ccagttcatc cggttccaga ctgtaattgg tttccatcgc cactttgcca
     7681 cgcagcactt tgcatttgca ggtcgcacag acgccgcctt tgcaggcata gggcagatca
     7741 gccccctggc gcaatgccgc atcgagaatg ctttcatcgt cggcattaag cacgatttcc
     7801 cgatcccgcc catcctgacg cacagtcact ttttgtccgt cactttgcac gttaacgtta
     7861 cgtttgacgc gcgtgccagc cgtattaaac cgctccagat gaatcgcttt ttctggcatt
     7921 cccagtgctt ttaaggcagc ttccgcgtca tccatcatcg ccgccggacc acaaataaac
     7981 gcctcatcat aaagacgaaa attaatgagc gaggccccaa gtgactgtaa tttttcaccg
     8041 tcaatacgcc cgtgaagcag atcgctgtcg agagtttcct gactgaaaat gcacaacaac
     8101 tgtaaacgct gaggatattt gtctttcagg tctgccagtg cctggcgaaa catcatgctc
     8161 tggctggtac ggttaccgta aatcagagtg aactgacttt caggctcggt ttgtaaagtg
     8221 gtggcgataa tcgccagcat tggcgtaata cccgatcctg ctgcaatagc cagatagcgg
     8281 ccctggcgtt cggcctgtgg ctgatagccg aaatgcccct gcggcaccat gacttccagc
     8341 gtcatcccct ggcggatgtg ttcgcgggca tagcgggaga aacgtccgcc ttcaatggct
     8401 ttcaccgcca cactaatttc gccaggcagg tagctacggc aaatggagta acaacggcgt
     8461 aattcttcac cattaaggct ggcttttaag gtcaaatgtt gaccgggacg aaagcgatac
     8521 gcctcctgca aaggctgggg taccgcaaag gtaatggtta ccgcatcacg ggtttctggc
     8581 tccacttttg ccaccgttaa ggaatgaaac gttgtcatgg catcctcaaa tacatttgaa
     8641 ataatcgaaa ggttcgcgac agctatcgca gcgatacaat gctttgcagg ccgtggaacc
     8701 aaattcactg ataagtgtgg tatggacgct ggcgcaacgc gggcaacgta cttctggcgg
     8761 caaatgggca tggcaactgt gtccggcggg cgggctaatg ccatactgtc gcagacgctc
     8821 acgggcatcg ggggtcatcc agtcggtggt ccacgccggg tcgagttgca gcacaacctg
     8881 aacgggggta aacccgttgg ttgtcattgc ctcacgtatc gcgccaatca aatgttccgt
     8941 tgccggacaa ccggaatatg tcggcgtaaa gccgatcacc catccttctc ccatctgtgt
     9001 cacattacgc accatgccta aatcagtaat ggtcagcacc gggatctccg gatccgggat
     9061 ctggctgagt agcgcccata tctcatgcac ctgtggcggt gcaatggtgg ccagacgttg
     9121 catacccatc tcctctgtta ccattgctga ccgggtaaga cacgctggag atactgcatt
     9181 tctgccacca tgggtccaag gtgttcggta tgcagtcctt ttttaccgcc agtgcgatac
     9241 gcctgctctt gcggtacgtt caatgtggct tcgttgaacc cagcaaaaac ttcggcttcc
     9301 cacgctgcgc gtaaagtgcg tgggtcaacc gcaataccct cttcactcag tgcaatatca
     9361 atctcgtcgg catcgaacag ttcggcggta aaacgccaca acttgttgat ggcctgctgc
     9421 atcttttgcc ctgatacgtc agtaccattg cccagtcgct ccagccagcc acgactaaaa
     9481 cgcaggtgat agcgagcttc tttaattgct ttggcagaaa tcgccgccag ttgcggctcg
     9541 cggctttcca tcagacgggt aaagagcgcc acatgccatg catcgatgaa atactgacgt
     9601 gcaatggtgt cggcaaaatt gccgtttggt tgttcaacca acaataagtt gctgaactgg
     9661 cgctcgtctc gggtgaaggc cagggtatct tcatcgcctt ctcccgctaa ttcagcggca
     9721 tacgataaga agttgcgtgc ctgacctaat aaatcgaggc caatgtttgc cagtgcgaga
     9781 tcgatttcca gttccggtgc gtgaccgcac cattcaccca accgctggga gagcaccagg
     9841 cagttatcgc ccaggcgcaa ggtgtaagcc gttaactgat tcatttccga cctcacatgt
     9901 gctcaatgcc atcagggatg gtgtaaaacg ttggatggcg atagaccttg ctttcagccg
     9961 gatcaaaaaa ttcaccgcgt tcttccggtt gcgaggcaac aatttcactc gccttcacca
    10021 cccaaattga acatccttcg ctacgacggg tataagcatc acgggcattt tccagtgcca
    10081 tccgctcatc ggcagcatgt aaactgccaa catggcggtg tgataagccc tgtttgccac
    10141 gcacgaaaac ttcgtataac ggccagtaaa cattactcat tttgaaactc cttatgcgac
    10201 cttacaggca tgttgttttt gtgcatgggc cagcgcagct tcccgtaccc aggtaccttc
    10261 ttcccaggct ttacgtttgg cgtcgagccg ctcctgatta caaattccgc gaccgtttat
    10321 cacttcgtta aactcctgcc agtcgatctc accaaagcgg tagtgaccgc tttcagtatc
    10381 aaaatgcaga tccgggtcag gaacggtcat accgagcatt tcaacctgtg gaacggtgtt
    10441 atccacgaaa cgctggcgga gttcgtcgtt ggtgaaacgt ttgattttcc aggcgagact
    10501 tctggcgctg tttggcgagt tatcatcgtt tggcccgaac atcattaagg ctggccacca
    10561 gaaacggtta atggcgtctt gcaacatctg cttttgagct tcgctgccct gcgccagtgc
    10621 catgcaggct tcaaacccct gacgctggtg aaaactctct tctttgcaga ttttcaccat
    10681 tgctctggca tacgggccat aagaagtgcg gcataacgcc acctgattga cgatcgctgc
    10741 gccatccacc agccagccga taacaccgat atcagcccaa ctcaatgtcg gataattaaa
    10801 gatggaggaa tatttcatcc gtccgtcgag cattttttgg tagatgtctt ctctggcgca
    10861 gcccagtgtt tcggcggcgc tatagagata cagaccatga cctgcttcat cctgcacttt
    10921 ggccaacaga atggctttac gccgtaaggt tggtgcgcga gtgatccagt taccttcagg
    10981 caacatacca acaatctcgg agtgcgcatg ctgcccaatc tggcggatca atgtctttcg
    11041 gtaagcatcg ggcatccagt cctgcggctc gatagccgtt tcctgggcta tccgttgctc
    11101 aaagcgttct tcttgggtca cgtcatcacc tttacgattc cagttttcgt gattcaatca
    11161 aaaacacgaa tcacattgtt ttatgaaagt tacacagtgg ttaactaaaa tcacaaattt
    11221 tgtatatttg aactgtgacg gatttcgcac cctattttta taaatcatta tttatcagtg
    11281 tattaatgtt tttcgcagtt gctatacaga tcgcacagtt aacatttcgt taaaagacca
    11341 cttgcttttt atgattcgcg atttaactat tagcaacgga aatgtgaaac atctggagag
    11401 tagcgatgca gcagttagcc agtttcttat ccggtacctg gcagtctggc cggggccgta
    11461 gccgtttgat tcaccacgct attagcggcg aggcgttatg ggaagtgacc agtgaaggtc
    11521 ttgatatggc ggctgcccgc cagtttgcca ttgaaaaagg tgcccccgcc ctccgcgcga
    11581 tgacctttat cgaacgtgcg gcgatgctta aagcggtcgc taaacatctg ctgagtgaaa
    11641 aagagcgttt ctatgctctt tctgcacaaa caggcgcaac gcgggcagat agttgggttg
    11701 atattgaagg cggtattggg acgttattta cttacgccag cctcggtggc cgggagctgc
    11761 ctgacgatac gctgtggccg gaagatgaat tgatcccctt atcgaaagaa ggtggatttg
    11821 ccgcgcgcca tgtactgacc tcgaagtcag gtgtggcggt gcatattaac gcctttaact
    11881 tcccctgctg gggaatgctg gaaaagctgg caccaacgtg gctgggcgga atgccagcca
    11941 tcataaaacc agctaccgcg acggcccaac tgactcaggc gatggtgaaa tcaattgtcg
    12001 atagtggtct tgttcccgaa ggcgcaatta gtctgatctg cggtagtgcg ggcgaccttt
    12061 tggatcatct ggacagccag gatgtggtga ctttcacggg gtccgcgacg accggacaga
    12121 tgctgcgagt tcagccaaat atcgttgcca aatctatccc cttcactatg gaagctgatt
    12181 ccctgaactg ctgcgtactg ggcgaagatg tcaccccaga tcaaccggag tttgcgctgt
    12241 ttattcgtga agttgtgcgt gagatgacca caaaagccgg gcaaaaatgt acggcaatcc
    12301 ggcggattat tgtgccgcaa gtattggtta atgctgtcag tgacgctctg gttgcgcgat
    12361 tacagaaagt cgtggtcggt gatcctgcgc aggaaggcgt gaaaatgggc gcactggtaa
    12421 atgctgagca gcgtgccgat gtgcaggaaa aagtgaacac attgctggct gcaggatgcg
    12481 agattcgcct cggtggtcag gcggatttat ctgctgcggg tgccttcttc ccgccaacct
    12541 tattgtactg tccgcagccg gatgaaacac cggcggtaca tgcaacagaa gcctttggcc
    12601 ctgtcgcaac gctaatgcca gcacaaaacc aacaacatgc tctgcaactg gcttgtgcag
    12661 gcggcggtag ccttgcggga acgctggtga cggctgatcc gcaaatagcg cgtcagttta
    12721 ttgccgacgc ggcacgtacg catgggcgaa ttcagatcct caatgaagaa tcggcaaaag
    12781 aatccaccgg gcatggctcc ccactgccac aactggtaca tggtgggcct ggtcgcgcag
    12841 gaggcggtga agaattaggt ggtttacggg cggtgaaaca ttacatgcag cgaaccgctg
    12901 ttcagggtag tccgacgatg cttgccgcta tcagtaaaca gtgggtgcgt ggtgcgaaag
    12961 tcgaagaaga tcgtattcat ccgttccgca aatattttga ggagctgcaa ccaggcgaca
    13021 gcctgttgac tccccgccgc acaatgacag aggccgatat tgtgaacttt gcttgcctca
    13081 gcggcgatca tttctatgca catatggata agattgctgc tgccgaatct atttttggtg
    13141 agcgtgtagt acatgggtat tttgtgcttt ctgcggctgc gggtctgttt gtcgatgccg
    13201 gagttggtcc ggtcattgct aactacgggc tggaaaactt gcgttttatc gaacccgtaa
    13261 aaccaggcga taccatccag gtgcgtctca cctgtaagcg caagacgctg aaaaaacagc
    13321 gtagcgcaga agaaaaacca acaggtgtag tagaatggtc ggtagaggta ttcaatcagc
    13381 atcaaacccc ggtggcgctg tattcaattc tgacgctggt ggccaggcag cacggtgatt
    13441 ttgtcgatta atcggtcaat gaagggcaac ggcgaatagt tgccctttta tttcaccaaa
    13501 ttttgtgacg ttgtcacatt atgcatgatg tgtatctgta ttttcagggc actcactgta
    13561 tgaaaagctg gcacacctgc caaacaacct ggcaggtgca ggcaatcccc tttacatcag
    13621 tactgataat gtgaacctga ctaaaccgcc cacagagcgc ggttgctaac aagaacacaa
    13681 catctgacga ggttaataat gggaagcccc tctctgtatt ctgcccgtaa aacagccctg
    13741 gcgttggcag tcgccttaag tttcgcctgg caagcgccgg tatttgccca cggtggtgaa
    13801 gcgcatatgg tgccaatgga taaaacgctt aaagaatttg gtgccgatgt gcagtgggac
    13861 gactacgccc agatctttac cctgattaaa gatggcgcgt acgtgaaagt gaagcctggt
    13921 gcgcaaacag caattgttaa tggtcagcct ctggcactgc aagtcccggt agtgatgaaa
    13981 gacaataaag cctgggtttc tgacaccttt attaacgatg ttttccagtc cgggctggat
    14041 caaacctttc aggtagaaaa gcgccctcac ccacttaatg cgctaactgc ggacgaaatt
    14101 aaacaggccg ttgaaattgt taaagcttcc gcggacttca aacccaatac ccgttttact
    14161 gagatctccc tgctaccgcc agataaagag gctgtctggg cgtttgcgct ggaaaacaaa
    14221 ccggttgacc agccgcgcaa agccgacgtc attatgctcg acggcaaaca tatcatcgaa
    14281 gcggtagtgg atctgcaaaa caacaaattg ctatcctggc aacccattaa agacgcccac
    14341 ggaatggtgt tgctggatga tttcgccagt gtgcagaaca ttattaacaa cagtgaagaa
    14401 tttgccgctg ccgtgaagaa gcgcggtatt actgatacga agaaagtgat taccacgccg
    14461 ctgaccgtag gttatttcga tggtaaagat ggcctgaaac aagatgcccg gttgctcaaa
    14521 gtcatcagct atcttgatgt cggtgatggc aactactggg cacatcccat cgaaaacctg
    14581 gtggcggtcg ttgatttaga acagaaaaaa atcgttaaga ttgaagaagg tccggtagtt
    14641 ccggtgccaa tgaccgcacg cccatttgat ggccgtgacc gtgttgctcc ggcagttaag
    14701 cctatgcaaa tcatcgagcc tgaaggtaaa aattacacca ttactggcga tatgattcac
    14761 tggcgtaact gggattttca cctcagcatg aattctcgcg tcgggccgat gatctccacc
    14821 gtgacttata acgacaatgg caccaaacgc aaagtcatgt acgaaggttc tctcggcggc
    14881 atgattgtac cttacggtga tcctgatatt ggctggtact ttaaagcgta tctggactct
    14941 ggtgactacg gtatgggcac gctaacctcg ccaattgccc gtggtaaaga tgcaccgtct
    15001 aacgcagtgc tccttaatga aaccattgcc gactacactg gcgtgccgat ggagatccct
    15061 cgcgctatcg cggtatttga acgttatgcc gggcctgagt ataagcatca ggaaatgggc
    15121 cagcccaacg tcagtaccga acgccgggag ttggtggtgc gctggatcag tacagtgggt
    15181 aactatgact acatttttga ctggatcttc catgaaaacg gcactattgg cattgatgcc
    15241 ggtgctacgg gcatcgaagc ggtgaaaggt gttaaagcga aaaccatgca cgatgagacg
    15301 gcgaaagatg acacgcgcta cggcacgctt atcgatcaca atatcgtggg tactacacac
    15361 cagcatattt ataatttccg cctcgatctg gatgtagatg gcgagaataa cagcctggtg
    15421 gcgatggacc cggtggtaaa accgaatact gccggtggcc cacgcaccag taccatgcaa
    15481 gttaatcagt acaacatcgg caatgaacag gatgccgcgc agaagtttga tccgggcacg
    15541 attcgtctgt tgagtaaccc gaacaaagag aaccgcatgg gcaatccagt ttcctatcaa
    15601 attattcctt atgcaggtgg tactcacccg gtagcaaaag gtgcccagtt cgccccggac
    15661 gagtggatct atcatcgttt aagctttatg gacaagcagc tttgggtaac gcgttatcac
    15721 cctggcgagc gtttcccgga aggcaaatat ccgaaccgtt cctctcatga caccggtctt
    15781 ggacaataca gtaaggataa cgagtcgctg gacaacaccg acgccgttat ctggatgact
    15841 accggcacca cacatgtggc ccgtgccgaa gagtggccga ttatgccgac cgaatgggta
    15901 catactctgc ttaaaccatg gaacttcttt gacgaaacgc caacgctagg ggcgataaag
    15961 aaagataagt gattgtttca gactaaaaaa acggcacctg gtgccgtttt ttacttatgc
    16021 gcgaaccaga ttaataccgt acacacaccg acttggtttc acaccaaccg tccagccagt
    16081 ccgggccaaa atcacggcct gttcctgact gcttcatccc accaaacggt aagttagcgt
    16141 caattaaggt atgactgttt acccacaccg tacctgcctg taagcgatcg ctatattcca
    16201 gagcctggga gagattttgc gtccagacgc tggcagttaa gccatattcc gtgtcgtttg
    16261 ccagttgtaa cgcctcttct ccatccgcta cacgcaccag gttaaccacc ggaccaaaca
    16321 cctcttcacg agttaagcgc aatttagcat tgggatttac caccagcgtt ggcgcaacat
    16381 aatatccctc tccggcaggt ccactcgccc cgcgaatcag ctctgcttgc tgtgcctgcg
    16441 catcgtcaag gaatgaacgc actttgtcgc aatgcgcacg agaaaccaaa gggttaatct
    16501 gtgcaacagg tgacatcccc ggtcccactt gcaacgattt taccgcctgc tcaaatccac
    16561 taaccagcgt gtcaaacaac ggcgcttcaa tataaattcg cgaactggcg gcgcatactt
    16621 gcccctgatt caggaaactt ccggtcatca agccttcaat aacccattgc ggatcagcat
    16681 cttttaatac aattgccggg tttttaccgc ccagttccag cgttacacgc gtcaagcgat
    16741 cagcagcagt tctggcaata cctttgcccg tcgcggttga accggtaaaa ctgattttcg
    16801 caacatgagg atgtgacgtc agggccgcgc cgcatacaga acctgacccg gtgaccacat
    16861 taaaaacgcc atcaggaata cctgcctcgc tggccagttc cgccacgcgc aacatagtca
    16921 gtggcgtggt ttccgatggc ttaatcacga ttgaacagcc tgctgccagt gctggcatca
    16981 ccttccacat gccaatcatc aacggaaagt tccatggcac aattcccgct accacgccaa
    17041 ccggctcttt acgcgtccag gcctgataac gcgccccctg gggtaaggga atcgacaagt
    17101 ccagcgtttt acccgcgatt ttggtcgtta acccggcggt ataacgcatc cagttcagcg
    17161 tacagcccac ttcaaaagca cgggaaatag caattgactt accttgctcc agggtttcca
    17221 gctgtgccag ctcctcaccg tgctgttcca ccagatcggc aaaacgtagc aggatacgtt
    17281 cacgctctgc gggtaatcgc ccggcccagc gacgcgagac aaaggcccgc caggcagaca
    17341 tgaccgcgtt atctacatcc gcttcgttgg catcaacagt tgacgcaatt tcttgcccgg
    17401 tggccggatc aaagatcgcc aaccgttttt cactttgtgc ggggccagga cgaccatcaa
    17461 tataaagacc gtgttgacga tcgagaaact gttggacctg gcttaatact gctacatgcg
    17521 gctctgtcat cacttttcct tattatttac ccagtgtgat ttcagtgtac gcagcgacaa
    17581 gaaaagtcac ttttttcgct gtgacattcg ctttgtcagc tgtgacaagc tccgcaaatc
    17641 gtgacaataa ccgcttatgt ctctatgcct gacaagactt atttcctata gtcaggctca
    17701 ttagcttcgt ttattgcaac acaaatgcaa caataaaaat acatttcaca gagcgaaaac
    17761 gtgccatgaa ccccgcagtg gataatgagt ttcagcaatg gctttcccaa atcaatcagg
    17821 tatgcggaaa ttttaccgga cgcctgctga ctgagcgtta cacgggtgta ctggacacgc
    17881 attttgccaa aggactaaag ctgagtaccg tgacaaccag cggggtgaat ttatcccgca
    17941 cctggcagga agtaaaaggc agcgacgatg cctggtttta caccgttttt cagcttagtg
    18001 gtcaggcaat aatggagcag gatgagcgtc aggtgcagat tggcgctggc gatattacgt
    18061 tactcgatgc ctcacgcccc tgttcgcttt actggcagga gtcttctaaa cagatttcat
    18121 tacttttacc acgcactctg ctggaacaat attttcccca tcaaaaacct gtctgcgcag
    18181 aaagactgga cgctgactta cctatggtgc aactcagtca tcgcctgttg caggagagca
    18241 tgaataaccc ggcactttct gaaacagaaa gtgaagctgc gctacaggcg atggtgtgtc
    18301 tgcttcgccc ggtacttcat cagcgggaat ctgttcaacc tcgccgtgaa cgtcagtttc
    18361 aaaaagtggt tacgttgata gacgataata ttcgcgaaga gatattacgc ccggagtgga
    18421 tagccggaga gacaggtatg tcagtacgta gtttgtaccg aatgtttgcc gataaaggtt
    18481 tggtggtcgc gcaatatatt cgtaaccgtc gtctcgattt ttgtgctgat gcgattcgcc
    18541 atgccgcaga tgatgaaaaa ctggcaggta ttggctttca ttggggattt tccgatcaga
    18601 gtcatttttc aacggtattt aagcaacgct ttgggatgac gccaggcgag tatcgacgta
    18661 aattccgcta actaattcgc gttgcatgta cagcaacgcg aattagttaa aacagatatt
    18721 tgactatgca tttttcaggg cgaaaggcaa tttgacagga atttttgaat attcaccttg
    18781 tttccggaaa atagaataat cgccttctcc tgtagcgtta tcccgatatt gcggttttgc
    18841 tattaaaatt ttcgcaccca tttaccgttt atcccttgta catattcccc cggtttagcc
    18901 cgagctacga gcttttgccc ggcgagtttc gcgatatcat ctaccgagac attattttgc
    18961 tttgccagtt gttggtaact cgctttacgt tcggcattaa tgtcgtttac taatttctct
    19021 gtttcagcat ctgttttcag cgcaaccaga taaccataaa atgtttcacc tacccgcccc
    19081 tgagttctgg cttcatccag agtcaatgcc attacattgc tgcttaccag cccaacaaga
    19141 aacgcacaaa gaagtaatgt tttcttcatc atcgcctcag aaaagatcgc tacgagtttc
    19201 aagcagctct tcgacatctt tgtctgcctt gatgatgatc tcatgctcaa ttttaacgtt
    19261 catattgata gtgatcggtt ccttaggtgc agcgacttca atgcgaggcg tacagccaac
    19321 cagcataaaa gatgacgtca acgcagccag taaaattttc attgtttttc ctcacactct
    19381 tttccttgcg gacagtcatt tcccggcaga cgtgcgttct gctccaacca tgcctggaga
    19441 ttatcgccaa agcgtaaact gcgccacagc gtaaaaatat tctcttcatg atggtaatta
    19501 aggtttaccg taccgctttt accatcaacg cgactggtac ctgtaatgtt ggcctgcatg
    19561 gttaataaac cgagattatc taaattaata tttgtcgatg aacggctaat ttccatatag
    19621 cgcaaccagt taattgctga acccgcagtc atattgtctt tcaccaccgc atctgcggtg
    19681 tctttatcca gccgcaaggt cattggcccg ctatttgcca gccagccatc tttcactatc
    19741 catttttcgt tgtttagcca taacggcaat gcaccactaa atgccccgga tatggcgaat
    19801 tgtttcggat tgacggcgct aaccagttcg ctggatgaga gattattcag ccgcaacagc
    19861 gccgggtcat gttgcggcat acgtaattgt ttcatcacca cgttaccgcc taacacatcg
    19921 acgctaacat cagtcaacag caatggttct tcttctgtcc acggatagcg cccttgcaaa
    19981 tcagccgtaa tatttttcgc tgtcaccaga ttaatcactt cggcaattcg caacgtaacg
    20041 gggccgcgag tccccagatg ccaggctcca tcggcaaaac ggaaaggcag gacaaaatcg
    20101 acaccgttaa cctgattatc tggcatccag gcactaccgc ctttcaacac accgtgccct
    20161 cccgcgcgga atccttgttc aggcgcagca gaaaatgcga cctgagcata cagttcgcca
    20221 tcgcgtaagt tcatcttcca gtcgggtggc accagcggct ggaatacggt cagtgattgt
    20281 ttaggccacc aggcgttacc gcgcagacga ataccgtccc agcgaccatt tacccgaacc
    20341 gggccaatct caccagcatg taaatcgcct ttaaagagga aatacgtcgg atcgcgtcca
    20401 tcgacgctaa attttaacgt tgatggcggt aacacactgc cgccagtgaa cagcgtttgc
    20461 ccggcgtcca gtgacagcgc gccgctgaag gagggatgct gtgcgtcacg tacccagacg
    20521 acgggcttgt cgagaattaa tcgcggcttt tctaccgtca tcgtaccgta ttgtaactgg
    20581 tcgaaaccgg tggagagatc ggtcagcgta atcgtgctgt catgccactc accctttcct
    20641 gcgacatccc aggtggcatt catcggtgta aaactgcctt ttccccagta gcgccactgc
    20701 cagcggccag cgtcagggag aaaatcattc gccagtccat ccatatgcag cacgaaatcg
    20761 cccagttcat tttcatgcgc ctgcaaaatg gcctgcaaac gtccgtcaac accacgttgg
    20821 gtgactttta cacccgctaa aggccagcgg atttcatcga tgtccagcga atcgatgact
    20881 cttcccttcg aacgtaataa cgcacctggc tcaaaggcca acgttgggtc agtcagactt
    20941 ccgcttagct gcgcaggtaa acgggcatat aaaatgaggt ccgcctgttt cgcttcaccg
    21001 gtcagttgca gaggcagctg gctgttatcc atgcttaatt ttcctgggcc aaaattaagc
    21061 accgcgttgc ccttacccgc ttgcccctgg gtcagcacac tcagtcgtcc gctgaccaga
    21121 gcgttctcaa gccctgcctg ccagttgtcg actttaacac ccagtcggcc actcaaagga
    21181 aaacctgcat acggccagct ccagcgacca tcgcttacgg tcaattgttg acgagtaatt
    21241 tgccacggca gatcgagcaa cggatcgccg ttatcccgtg ccaccacaat caattgcccg
    21301 ctattttcct gccagtccag ctcggcatcc accagtgaag gctcctgcga caagtttaac
    21361 gtcgcagtag catgaccact tacaggaagt ccatccggca ctagcggcat agtaaattcc
    21421 cccaccagtt ttaccggcgg ctgattttca aacgcgacga catccagttc gctgactgta
    21481 agttgttgcc ctttcagctg gccttgaaat ttaacttttt cgccctgata acgcagttgc
    21541 tggatatcgg aggttaatgc gagagagagt tttccctgcc actcctgcca gggagaaaaa
    21601 atcagtttat cgatattgat ccaggtgtta ggcaacatgg cctgccactg cgcgagggtt
    21661 tttggagcgg caagcgattg ctccgtctgc ggcaatttcg ccagacaagc agaatcaaat
    21721 tctaccgtgc cgacgttcaa taaccagcgg ctgggatgtg aaagactggc gttggtgata
    21781 tgcgcaagct gacaatctcc caccagataa cggagatcgg gaatgattaa acctttacgc
    21841 gtaatgcgtg ggctttcatc taatgcaata cgtgtcccga gcggtagcca gatgcccgcc
    21901 agcgtgggaa cccacaagcc gagcgtcatc agcagcgtca acggcacaag aataatcagt
    21961 aataacagcg cgagaacggc tttatattta cccagcatgg gtagttaata tcctgattta
    22021 gcgaaaaatt aagcattcaa tacgggtatt gtggcatgtt taaccgttca gttgaaggtt
    22081 gcgcctacac taagcatagt tgttgatgaa tttttcaata tcgccatagc tttcaattat
    22141 atttgaaatt ttgtaaaata tttttagtag cttaaatgtg attcaacatc actggagaaa
    22201 gtcttatgaa actcgccgtt tatagcacaa aacagtacga caagaagtac ctgcaacagg
    22261 tgaacgagtc ctttggcttt gagctggaat tttttgactt tctgctgacg gaaaaaaccg
    22321 ctaaaaccgc caatggctgc gaagcggtat gtattttcgt aaacgatgac ggcagccgcc
    22381 cggtgctgga agagctgaaa aagcacggcg ttaaatatat cgccctgcgc tgtgccggtt
    22441 ttaataacgt cgaccttgac gcggcaaaag aactggggct gaaagtagtc cgtgttccag
    22501 cctatgatcc agaagccgtt gctgaacacg ccatcggtat gatgatgacg ctgaaccgcc
    22561 gtattcatcg cgcgtatcag cgtactcgtg acgccaactt ctctctggaa ggtctgaccg
    22621 gctttactat gtatggcaaa acggcaggcg ttatcggtac cggtaaaatc ggtgtggcga
    22681 tgctgcgcat tctgaaaggt tttggtatgc gtctgctggc gttcgatccg tatccaagtg
    22741 cggcggcact ggaactcggt gtggagtatg tcgatctgcc aaccctgttc tctgaatcag
    22801 acgttatctc tctgcactgc ccgctgacac cggaaaacta ccatctgttg aacgaagccg
    22861 ccttcgatca gatgaaaaat ggcgtgatga tcgtcaatac cagtcgcggt gcattgattg
    22921 attctcaggc agcaattgaa gcgctgaaaa atcagaaaat tggttcgttg ggtatggacg
    22981 tgtatgagaa cgaacgcgat ctgttctttg aagataaatc caacgacgta attcaggatg
    23041 acgtattccg tcgcctgtct gcctgccaca acgtgctgtt taccgggcat caggcattcc
    23101 tgacagcaga agctctgacc agtatttctc agactacgct gcaaaactta agcaatctgg
    23161 aaaaaggcga aacctgcccg aacgaactgg tttaatcttg ccgctcccct gcattccagg
    23221 ggagctgatt cagataatcc ccaatgacct ttcatcctct attcttaaaa tagtcctgag
    23281 tcagaaactg taattgagaa ccacaatgaa gaaagtagcc gcgtttgttg cgctaagcct
    23341 gctgatggcg ggatgtgtaa gtaatgacaa aattgctgta accccagaac agctacagca
    23401 tcatcgcttt gtgctggaaa gcgtaaacgg taagcccgtg accagcgata aaaatccgcc
    23461 agaaatcagc tttggtgaaa aaatgatgat ttccggcagc atgtgtaacc gctttagcgg
    23521 tgaaggcaaa ctgtctaatg gtaaactgac agccaaaggg ctggcaatga cccgtatgat
    23581 gtgcgctaac ccgcagctta atgaactcga taacaccatt agcgaaatgc tgaaagaagg
    23641 tgcacaagtg gatctgaccg cgaaccagtt aacgctggcg accgcgaaac agacattaac
    23701 ttataagcta gcggatttaa tgaattaata gctgccacag ctcccggcgg caagtgactg
    23761 ttcgctacag cgtttgccgt tgggtaatgc acacatccca atcgccgtac catccagttg
    23821 acgggcaaca gaaagcgaac cgccgatcat tgcacaattt gcttctccac tactggacat
    23881 cgacgctttt aaacctggcg ctacgtgcgc cgcagtcgcc tgctgaacag gttcactact
    23941 acacgccgac aacaataaag cggcacaccc tacccaaaac gctgctcgca tcttttcttc
    24001 ctctgatctt caagccaaac gacaccgcca taaataatag gcagcacaga gggcgacgtc
    24061 gagagctgtc ctgcgcgttg ccccgcattt ttactttttt atggctattt ttttgccctc
    24121 tgtttgatca aaacattcat tacgctgatg tgggggacac aaaagcgaaa atgcagaaga
    24181 aagccatttg ctaaaattga aggattacta ctgggcgcgc agcaatttcg tgcgcccctc
    24241 atttgcgcaa tgtaagggtg tcatatgatt actattgacg gtaatggcgc ggttgcttcg
    24301 gtggcgtttc gcaccagtga agttatcgcc atctacccta ttacccccag ttccacgatg
    24361 gcagaacagg ctgatgcctg ggccggaaac ggcttgaaaa acgtttgggg agacacacca
    24421 cgcgtggttg aaatgcagtc ggaagcgggg gctatcgcta ccgtgcatgg cgctttgcag
    24481 acgggtgctc tttcaacatc gtttacgtca tcgcagggtt tgctgctgat gatcccgacg
    24541 ctgtacaaac tggcaggcga actcacaccg tttgtcctgc atgtagcggc acgtaccgtt
    24601 gccacacatg cactctctat ttttggcgat cattccgacg ttatggcggt gcgccagacg
    24661 ggttgcgcga tgttgtgtgc ggcaaacgtc caggaagcgc aagactttgc tctcatttcg
    24721 cacatcgcga cgctgaagag ccgcgtgcca tttattcatt tctttgatgg tttccgcacg
    24781 tcccacgaaa tcaataaaat tgtcccgctg gccgatgaca cgattcttga actgataccg
    24841 caggcagaaa ttgatgccca ccgcgcccgc gggctgaatc cggagcatcc ggtgattcgc
    24901 ggtacctctg ctaacccgga cacctacttc cagtcacgtg aagccaccaa cccgtggtac
    24961 aacgcagttt acgaccatgt tgaacaggcg atgaatgatt tcgctgccgc gacaggtcgt
    25021 cagtatcagc cgtttgagta ttacgggcat ccgcaagcgg aacgggtgat tatcctgatg
    25081 ggctctgcca ttggcacctg tgaagaagtg gttgatgaat tgctaacccg tggcgaaaaa
    25141 gtcggcgtgc tgaaagttcg cctgtaccgt cccttctccg ccaaacactt gctacaagct
    25201 ctgccgggat ccgtacgcag cgtggcggta ctggacagaa ccaaagaacc cggtgcccag
    25261 gcagaaccgc tctatctgga tgtaatgacc gcactggcag aagcctttaa taatggcgag
    25321 cgcgaaactc tgccccgtgt cattggtggg cgctatggtc tttcatccaa agaatttggc
    25381 ccggactgtg tactggcggt atttgccgag ctcaacgcgg ctaaaccgaa agcgcgcttt
    25441 acggttggta tttacgatga tgtgaccaat ctgtcactgc cgttgccgga aaacaccctg
    25501 ccaaactcgg cgaaactgga agccttgttt tatggccttg gtagtgatgg cagcgtttcc
    25561 gcgaccaaaa acaatatcaa gattatcggt aattccacgc cgtggtacgc acagggctat
    25621 tttgtttacg actccaaaaa ggcgggcggc ctgacagttt ctcaccttcg cgtgagcgaa
    25681 cagccgattc gttccgctta tctcatttcc caggctgatt ttgttggctg ccaccagttg
    25741 cagtttatcg acaaatatca gatggttgag cgtttaaaac ctggcggcat tttcctgctc
    25801 aacacgccgt acagcgcaga tgaagtgtgg tcgcgcttgc cgcaggaagt tcaggccgtg
    25861 ctaaaccaga aaacagcgcg cttctatgtg attaacgcag cgaaaatcgc ccgcgaatgt
    25921 ggcctggcgg cccgtattaa taccgtcatg cagatggctt ttttccatct gacgcaaatt
    25981 ctgcctggcg atagcgccct cgcagaattg cagggtgcga ttgccaaaag ttacagtagc
    26041 aaaggccagg atctggtgga acgcaactgg caggctctgg cactggcgcg tgaatccgta
    26101 gaagaagttc cgttgcagcc ggttaatccg cacagcgcca atcgaccgcc tgtggtttcc
    26161 gatgccgctc ctgatttcgt gaaaaccgtt accgcggcga tgctcgccgg gcttggtgat
    26221 gccctccccg tttcggcgct gccgccagac ggcacctggc cgatgggcac cacacgctgg
    26281 gaaaaacgca atatcgccga agagatcccc atctggaaag aggaactctg tacccaatgt
    26341 aaccactgcg tcgccgcttg cccacactca gctattcgcg caaaagtggt accgcctgaa
    26401 gcgatggaaa acgcccctgc cagcctgcat tcgctggatg tgaaatcgcg tgatatgcgc
    26461 gggcagaaat atgtcttgca ggtggcaccg gaagattgca ccggctgtaa cctgtgcgtc
    26521 gaagtttgcc cggcgaaaga ccgtcagaat ccagagatta aagctatcaa tatgatgtcg
    26581 cgcctggaac atgtcgaaga agagaaaatc aattacgatt tcttcctcaa cctcccagaa
    26641 atcgaccgta gcaaactgga acgtattgat attcgtacat cgcagctgat tacaccgctg
    26701 tttgaatatt caggtgcttg ctccggttgt ggcgagacgc cgtatattaa attgctgact
    26761 cagctgtatg gcgaccggat gttgatcgct aatgccactg gctgttcttc aatatacggc
    26821 ggtaacctgc cctctacacc gtataccacc gataccaacg gtcgcgggcc ggcatgggcg
    26881 aactcgctgt ttgaagataa cgccgaattt ggccttggtt tccgcctgac ggtcgatcaa
    26941 caccgtgtcc gagtgctgcg tctgctcgat caatttgccg ataaaatccc ggcagaatta
    27001 ctgactgcgt tgaaatcaga cgccacgcca gagattcgtc gagaacaggt cgcagcttta
    27061 cgccagcaac tcaacgatgt tgccgaagca catgaactgc tacgtgatgc agatgcactg
    27121 gtggaaaaat caatctggct gattggtggt gacggctggg cttacgatat cggctttggc
    27181 ggtctggatc atgtattgag tttgacggaa aacgtcaaca ttctggtgct ggatacgcaa
    27241 tgctattcca ataccggtgg tcaggcgtca aaagcgacac cgctgggtgc agtaactaag
    27301 tttggcgagc acggcaaacg taaagcgcgt aaagatcttg gcgtcagtat gatgatgtac
    27361 ggccatgttt atgtggcgca gatttctctc ggcgcgcagc tgaaccagac ggtgaaagca
    27421 attcaggaag cggaagcgta tccgggacca tcgctgatca tcgcttatag cccgtgtgaa
    27481 gagcatggtt acgatctggc tctcagccac gaccagatgc gccaactcac tgctaccggc
    27541 ttctggccgc tatatcgctt cgatcctcgt cgtgccgatg aaggcaaact gccgctggcg
    27601 ctggattcac gcccgccgtc agaagcactg gaagaaacgt tacttcatga gcaacgtttc
    27661 cgtcgtctga attcgcagca gccagaagtg gcagaacagt tatggaaaga tgctgcagct
    27721 gatttgcaaa aacgctatga cttcctggca caaatggccg gaaaagcgga aaaaagcaac
    27781 accgattaaa tgcactggat aaggattatc caattctaaa aaaaagcccg gacgactgtt
    27841 cgggcttgtc tcattatttg ttggataaac agtaacaagg gtccaacaga aaataatctt
    27901 tgtgaca
//
LOCUS       DACBVI010000050        27541 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.050, whole genome shotgun sequence.
ACCESSION   DACBVI010000050 DACBVI010000000
VERSION     DACBVI010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.050"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            227..2650
                     /gene="pgaA"
                     /locus_tag="IU325_10445"
     CDS             227..2650
                     /gene="pgaA"
                     /locus_tag="IU325_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HAP5809441.1"
                     /translation="MYSSSRKRCPKTKWALKILTAAFLAASPAAKSAANNAYDALIIE
                     ARKGNTQPALLWFAQKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLESQNKDYQRGQILTLADAGHYDSALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHEDELRAMTGSLPENALTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWAQYWVASAYLKEHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLNDNWRGFAGFGYADG
                     QFSEGKGIIRDWLAGVEWRSRNIWLEAEYTERFFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPNLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2659..4677
                     /gene="pgaB"
                     /locus_tag="IU325_10450"
     CDS             2659..4677
                     /gene="pgaB"
                     /locus_tag="IU325_10450"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HAP5809442.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHQGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADEQVKFGDEMVD
                     REYFATWQQVREVARSRLVEVASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAKVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYRRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWTR
                     FKSRALTDFTLELSARVKAIRGPHVKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYMEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDVIRPEFSTAWYPKND"
     gene            4670..5995
                     /gene="pgaC"
                     /locus_tag="IU325_10455"
     CDS             4670..5995
                     /gene="pgaC"
                     /locus_tag="IU325_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HAP5809443.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWAIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            5997..6410
                     /gene="pgaD"
                     /locus_tag="IU325_10460"
     CDS             5997..6410
                     /gene="pgaD"
                     /locus_tag="IU325_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HAP5809444.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6460..7383)
                     /gene="phoH"
                     /locus_tag="IU325_10465"
     CDS             complement(6460..7383)
                     /gene="phoH"
                     /locus_tag="IU325_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HAP5809445.1"
                     /translation="MRSRFSAFYPKANYGLQGSQPSDVRAHNRAANGACDEYKQLKVL
                     SMGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEAIGMARDSRDTSPI
                     LARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHKDVDRIIVTRPVLQ
                     ADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIGKVEIAPFAYMRGR
                     TFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLPRGVRSGLSDALER
                     FEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7868..9139)
                     /gene="efeB"
                     /locus_tag="IU325_10470"
     CDS             complement(7868..9139)
                     /gene="efeB"
                     /locus_tag="IU325_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HAP5809446.1"
                     /translation="MQYEDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIASDNLTITLSVGHSLFDERFGLTPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQQVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTPESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(9145..10272)
                     /gene="efeO"
                     /locus_tag="IU325_10475"
     CDS             complement(9145..10272)
                     /gene="efeO"
                     /locus_tag="IU325_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HAP5809447.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDKYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10330..11160)
                     /locus_tag="IU325_10480"
     CDS             complement(10330..11160)
                     /locus_tag="IU325_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HAP5809448.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(11814..13322)
                     /gene="putP"
                     /locus_tag="IU325_10485"
     CDS             complement(11814..13322)
                     /gene="putP"
                     /locus_tag="IU325_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HAP5809449.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPSVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(13481..13690)
                     /locus_tag="IU325_10490"
     CDS             complement(13481..13690)
                     /locus_tag="IU325_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809450.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            13745..17707
                     /gene="putA"
                     /locus_tag="IU325_10495"
     CDS             13745..17707
                     /gene="putA"
                     /locus_tag="IU325_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HAP5809451.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPIAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQSVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDTLHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(17747..18385)
                     /gene="rutR"
                     /locus_tag="IU325_10500"
     CDS             complement(17747..18385)
                     /gene="rutR"
                     /locus_tag="IU325_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HAP5809452.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            18673..19764
                     /gene="rutA"
                     /locus_tag="IU325_10505"
     CDS             18673..19764
                     /gene="rutA"
                     /locus_tag="IU325_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HAP5809453.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGSGKS
                     DFKGDFFTMDDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            19764..20456
                     /gene="rutB"
                     /locus_tag="IU325_10510"
     CDS             19764..20456
                     /gene="rutB"
                     /locus_tag="IU325_10510"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HAP5809454.1"
                     /translation="MTILTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            20468..20854
                     /gene="rutC"
                     /locus_tag="IU325_10515"
     CDS             20468..20854
                     /gene="rutC"
                     /locus_tag="IU325_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HAP5809455.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            20862..21662
                     /gene="rutD"
                     /locus_tag="IU325_10520"
     CDS             20862..21662
                     /gene="rutD"
                     /locus_tag="IU325_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HAP5809456.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVIC
                     YDQRGTGNNPDTLEEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASLTVLVSVNGWLRINAHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            21672..22262
                     /locus_tag="IU325_10525"
     CDS             21672..22262
                     /locus_tag="IU325_10525"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HAP5809457.1"
                     /translation="MNEAISPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSIQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFT
                     GSTLKSNLLINIGYGDSSKLYARLPRLSFEEACGLL"
     gene            22273..22767
                     /gene="rutF"
                     /locus_tag="IU325_10530"
     CDS             22273..22767
                     /gene="rutF"
                     /locus_tag="IU325_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HAP5809458.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            22788..24116
                     /gene="rutG"
                     /locus_tag="IU325_10535"
     CDS             22788..24116
                     /gene="rutG"
                     /locus_tag="IU325_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HAP5809459.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAPVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(24199..24372)
                     /locus_tag="IU325_10540"
     CDS             complement(24199..24372)
                     /locus_tag="IU325_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein"
                     /protein_id="HAP5809460.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            24745..25341
                     /gene="wrbA"
                     /locus_tag="IU325_10545"
     CDS             24745..25341
                     /gene="wrbA"
                     /locus_tag="IU325_10545"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAP5809461.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            25362..25589
                     /locus_tag="IU325_10550"
     CDS             25362..25589
                     /locus_tag="IU325_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809462.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(25627..26868)
                     /locus_tag="IU325_10555"
     CDS             complement(25627..26868)
                     /locus_tag="IU325_10555"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HAP5809463.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(27160..>27541)
                     /locus_tag="IU325_10560"
     CDS             complement(27160..>27541)
                     /locus_tag="IU325_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809464.1"
                     /translation="FFKKEFIDSCLNNEKCILHLPEFIFNDNKNLLALDTDTSDRIKA
                     VKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLINTGNHYDVYLPPEL
                     FAQAYKLNNKEMNAQLDYLNRYAI"
BASE COUNT         6739 a         7115 c         6968 g         6719 t
ORIGIN      
        1 ttatgccgta ttcctgaagt tcctcatcat tggaatggat tttcgggcga gaaaaggatt
       61 ttatatggat actctactca tcatttcttc ttctcatcat caacaattca cgtctctctt
      121 ccgcgtttaa taacggatta tgatgtgcaa aaaaaaatat ctttcgtttc agttacctgt
      181 aattagatac agagagagat tttagcaata catggagtaa tacaggatgt attcaagtag
      241 cagaaaaagg tgcccgaaaa ccaaatgggc tttgaaaatt cttactgccg catttttagc
      301 agcgagtccc gcggcgaaga gtgctgctaa taatgcctat gatgcattga ttatcgaagc
      361 tcgcaaggga aatactcagc cagctttgtt atggtttgca caaaaatcag cactcagtaa
      421 taaccaaatt gctgactggt tacagattgc cttatgggcc gggcaagata aacaggttat
      481 taccgtttac aaccgctacc gtcatcagca attaccagcg cgtggttatg cagctgtcgc
      541 cgtcgcttat cgtaacctgc aacaatggca aaactcgctt acactgtggc aaaaggcact
      601 ctctctggag tcgcaaaata aggattatca acggggacaa attttaaccc tggcagatgc
      661 gggtcactat gattctgcgc tggttaaact taagcagctt aactctggag caccggacaa
      721 agctaattta ctcgcagaag cctatatcta taaactggcg gggcggcatg aggatgaatt
      781 acgggcgatg acagggtcat tacctgaaaa tgccttaacg caacaatatc ccacagaata
      841 cgtgcaggca ttacggaata atcaacttgc tgccgcgatt gacgatgcca atttaacgcc
      901 agatattcgc gctgatattc atgccgaact ggtcagattg tcatttatgc ctacgcgcag
      961 tgaaagtgaa cgttatgcca ttgccgatcg ggccctcgcc caatacgctg cattagaaat
     1021 tctgtggcat gataaccctg accgcactgc ccagtaccag cgtattcagg ttgatcatct
     1081 tggcgcgtta ttaactcgcg atcgttataa agacgttatt tctcactatc agcgtttaaa
     1141 aaagacgggg caaattattc cgccctgggc gcaatattgg gttgcgtcgg cttatctcaa
     1201 agaacatcag ccaaaaaaag cacagtcaat aatgacagag ctcttttatc acaaggagac
     1261 cattgccccg gatttatccg atgaagaact tgcggatctc ttttacagcc acctggagag
     1321 tgaaaattat cccggcgcgc taactgtcac ccaacatacc attaatactt cgccgccttt
     1381 ccttcggtta atgggcacgc ctacgagcat cccgaatgat acctggttac aggggcattc
     1441 gtttctctca accgtagcaa aatatagtaa tgatcttcct caggctgaaa tgacagccag
     1501 agagcttgct tataacgcac caggaaatca gggactgcgc attgattacg cgagtgtgtt
     1561 acaagcccgc ggttggcctc gtgcggcaga aaatgaatta aaaaaagcag aagtgatcga
     1621 gccacgtaat attaatctgg aggttgaaca agcctggaca gcattaacgt tacaagaatg
     1681 gcagcaggca gcggtcttaa cgcacgatgt tgtcgaacgt gaaccgcaag atcccggcgt
     1741 tgtacgttta aaacgtgcgg ttgatgtaca taatcttgca gagcttcgta tcgctggctc
     1801 aacaggaatt gatgccgaag gtccggatag tggcaaacat gatgtcgact taaccaccat
     1861 cgtttattca ccaccgctga atgataactg gcgcggtttt gctggattcg gttatgccga
     1921 tggacaattt agcgaaggaa aagggattat tcgcgactgg cttgcgggtg ttgagtggcg
     1981 gtcacgtaat atctggcttg aggcggagta caccgaacgc tttttcaatc atgagcataa
     2041 acccggcgcg cgcctgtctg gctggtatga ttttaatgat aactggcgta ttggttcgca
     2101 actggaacgc ctctctcacc gcgttccatt acgggcaatg aaaaatggtg ttacaggcaa
     2161 cagtgcacag gcttatgttc gctggtatca aaatgagcgg cgtaagtacg gtgtctcctg
     2221 ggctttcact gatttttctg acagtaatca gcgtcatgaa gtctcacttg agggtcagga
     2281 acgcatctgg tcttcaccat atttgattgt cgatttccta cccaatctgt attacgaaca
     2341 aaatacagaa cacgacactc catactacaa ccctataaaa acgttcgata ttgttccggc
     2401 atttgaggca agtcatttgt tatggcgtag ctatgaaaat agctgggagc aaatcttcag
     2461 cgcaggtgtt ggtgcctcct ggcaaaaaca ttatggcacg gatgtcgtca cccaactcgg
     2521 ctacgggcaa cgcattagct ggaatgacgt gattgatgct ggcgcaacgc tacgctggga
     2581 aaaacgacct tatgacggtg acagagaaca caacttatac gttgaattcg atatgacatt
     2641 cagattttaa ggataaatat gttacgtaat ggaaataaat atctcctgat gctggtgagt
     2701 ataattatgc tcactgcgtg cattagccag tcaagaacat catttatacc gccacaggat
     2761 cgcgaatctt tactcgccga gcaaccgtgg ccgcataatg gttttgtggc gatttcatgg
     2821 cataacgttg aagacgaagc tgccgaccag cgttttatgt cagtgcggac atcagcactg
     2881 cgtgaacaat ttgcctggct gcgcgagaac ggttatcaac cggtcagtat tgctcaaatt
     2941 cgtgaagcac atcaaggagg aaagccgcta ccggaaaaag cagtagtgct aacttttgat
     3001 gacggctacc agagttttta tacccgcgtc ttcccaattc ttcaggcctt ccagtggcct
     3061 gctgtatggg cccccgtcgg cagttgggtc gatacgccag cggatgaaca agtaaaattt
     3121 ggcgatgaga tggtcgatcg agaatatttt gccacgtggc aacaagtgcg agaagttgcg
     3181 cgttcccggc tcgttgaggt cgcttctcat acatggaatt ctcactacgg tattcaggct
     3241 aatgccaccg gcagcttatt gcctgtatat gtaaatcgtg catattttac tgaccacgca
     3301 cggtatgaaa ccgcagcaga ataccgggaa agaattcgtc tggatgctgt aaaaatgacg
     3361 gaatacctgc gtacaaaggc taaggtaaat ccacacgttt ttgtttggcc ttatggcgaa
     3421 gcgaatggca tagcgataga ggaattaaaa aaactcggtt atgacatgtt cttcaccctt
     3481 gaatcaggtt tggcaaatgc gtcacaattg gattccattc cgcgagtatt aatcgccaat
     3541 aatccctcat taaaagagtt tgcccagcaa attattaccg tacaggaaaa atcaccacaa
     3601 cggataatgc atatcgatct tgattacgtt tatgacgaaa accgccagca aatggatcgc
     3661 aatattgatg tgctaattca gcgggtgaaa gatatgcaaa tatcaaccgt gtatttgcag
     3721 gcatttgctg atcccgatgg tgatgggctg gtcaaagagg tctggtttcc aaatcgtttg
     3781 ctaccaatga aagcagatat ttttagtcgg gttgcctggc aattacgtac ccgctcaggt
     3841 gtaaacatct atgcgtggat gccggtatta agctgggatt tagatcccac attaacgcga
     3901 gtaaaatact taccgactgg ggagaaaaaa gcacaaattc atcctgaaca atatcgccgt
     3961 ctctctcctt tcgatgacag agtcagagca caagttggca tgttatatga agatcttgcc
     4021 ggacatgctg cttttgatgg catattgttc cacgatgatg ctttgctttc agattatgaa
     4081 gatgccagtg caccggctat cacggcttat cagcaagcag gctttagcgg gagtctgagc
     4141 gaaattcgac aaaacccgga gcaatttaaa cagtggaccc gctttaaaag tcgtgcgtta
     4201 actgacttca ctttagaact tagtgcgcgc gtaaaagcca ttcgcggtcc acatgttaaa
     4261 actgcacgaa atatttttgc acttccggta atacaacctg aaagtgaagc ctggtttgca
     4321 cagaattatg ctgatttcct aaaaagctat gactggaccg ctattatggc tatgccttat
     4381 atggaaggtg tcgcagaaaa atcggctgac caatggttaa tacaattgac caatcaaatt
     4441 aaaaacatcc ctcaggctaa agacaaatct attttagaat tacaggcaca aaactggcag
     4501 aaaaatggtc agcatcaggc tatttcttcg caacaactcg ctcactggat gagcctatta
     4561 caactgaatg gagtgaaaaa ctatggttat tatcccgaca attttctgca taaccaacct
     4621 gaaatagacg ttattcgtcc tgagttttca acagcctggt atccgaaaaa tgattaatcg
     4681 catcgtatcg ttttttatat tatgtctggt gttatgcata cccctatgcg tagcgtactt
     4741 tcactctggt gaactgatga tgaggttcgt tttcttctgg ccgtttttta tgtccatcat
     4801 gtggattgtt ggcggcgtct atttctgggt ctatcgtgaa cgccactggc cgtggggaga
     4861 aaacgcacca gctccccagt tgaaagataa tccgtctatc tccattatca ttccctgttt
     4921 taatgaggag aaaaacgttg aggaaaccat acacgccgct ttagcacagc gttatgagaa
     4981 cattgaagtt attgccgtaa atgacggttc aacagacaaa acccgtgcca tcctggatcg
     5041 catggctgca caaattcccc acctgcgggt cattcatctg gcgcaaaacc aggggaaagc
     5101 cattgcgctt aaaaccggag ctgccgcggc gaaaagtgaa tatctggtgt gcattgatgg
     5161 cgatgcgtta ttagaccgcg atgcggcggc atatattgtg gaaccgatgt tgtacaaccc
     5221 gcgtgtgggt gccgtaaccg gtaatcctcg tattcgaaca cgttctaccc tggtgggtaa
     5281 aattcaggtt ggcgagtatt cctcaattat tggtttgatc aagcgaaccc agcgtatcta
     5341 tggaaacgta tttaccgttt ccggtgttat tgccgcattt cgtcgcagcg ccctggcaga
     5401 agtgggttac tggagtgacg atatgatcac cgaagatatt gatattagct ggaagctgca
     5461 gttgaatcag tgggcgattt tttacgagcc acgggcgctg tgctggatat taatgcctga
     5521 aacgttaaaa gggctgtgga aacagcgcct gcgctgggct cagggcggtg cagaagtatt
     5581 cctcaaaaat atgacaaggt tgtggcgcaa agaaaacttt cgaatgtggc cgctgttttt
     5641 tgaatactgc ctgacgacaa tatgggcctt cacttgcctg gtcggtttca ttatttacgc
     5701 agtccaactt gccggtgtac cgttaaatat tgaattgaca catatcgctg cgacacatac
     5761 tgccggaata ttattgtgta cgttatgttt actgcaattt attgtcagcc tgatgatcga
     5821 gaatcgctat gagcataatc tgacttcatc gcttttctgg attatttggt tcccggttat
     5881 tttctggatg ctgagtctgg caacgacatt ggtatcattt acacgagtca tgttgatgcc
     5941 taaaaagcaa cgcgcccgtt gggtaagtcc cgatcgcggg attctgagag gttaatatga
     6001 acaatttaat tattacgacc cgacaatcgc cagtacgttt actggttgat tatgttgcca
     6061 caaccatctt atggacatta tttgctttgt tcatattctt attcgcaatg gatctgctga
     6121 cgggttatta ctggcaaagc gaggccagaa gccgacttca gttctatttt ttgctggcag
     6181 tggcgaatgc cgtcgtgtta attgtctggg cgctgtacaa taagctacgt tttcaaaaac
     6241 agcagcatca tgcagcctac caatatacgc cgcaagaata tgctgagagc ttagcaatac
     6301 ctgatgagct ctatcagcaa ctacaaaaaa gccacaggat gagcgtacac ttcaccagcc
     6361 aggggcaaat aaaaatggtg gtttcagaaa aagcgctagt ccgggcgtaa acacccaaaa
     6421 caaagcccgg ttcgcccggg ctctgcgccg ataatacact taactgtatg catgtagcgt
     6481 acgttggcaa agtgccgaac gtacgcaatc ctctttaccg aatcggacga tcccgaccat
     6541 ttcatcttct tcgaaacgtt ccagcgcgtc acttaatccg gagcgcacgc cgcgaggcaa
     6601 atcgcattgc gtgatatcac cgttgacgat aaccgtcacg ttctccccga ggcgggttaa
     6661 aaacattttc atttgcgcgg cagtcacatt ctgcgcctcg tcaagaatga cgactgcatt
     6721 ttcaaaggta cgtccacgca tataggcgaa cggcgcaatt tccaccttcc ctatttccgg
     6781 tcgcaggcag tactgcataa aggaagcccc taagcgccgg accagcacgt cgtagaccgg
     6841 acgaaaatag ggagcaaact tttctgcgat atcaccaggt aagaagccaa gatcttcatc
     6901 ggcttgcaga actggacggg tgacgataat cctgtcgaca tccttatgta tcagggcctc
     6961 tgccgctttt gctgcgctga tccaggtttt tccgcacccg gcttcgcccg tggcgaatat
     7021 cagctgctta ctctcaatag ccttcagata gtgcaattgc gcttcatttc gcgcgaggat
     7081 gggcgaggta tcgcgactgt cgcgggccat accaatggct tctacgccgc tcatctgcac
     7141 aagcgaggtg accgattctt cttcacgctg cttatggctg cgcgaatccc gtctcagcac
     7201 acgttttgcc tcgcgacgag ctttgatcac tgctttttgt cttcccatgg agagcacctt
     7261 gagttgtttg tattcatcac acgcgccgtt ggcagcgcga ttatgcgcac gaacatcaga
     7321 gggttggctt ccttgtaagc catagtttgc ttttggataa aatgccgaaa aacggctacg
     7381 cacaccgttt gcggcgtcgg taacacgagt aaagagagga agtgagaaaa atacagagtg
     7441 acgaagttgc taccggagga gaaatctccg cgagtggtgg agccttgctt aacaaaacat
     7501 tgtccagaac aggacgttac catccgcgat ctccatagtg actgactatc actgccggga
     7561 acttccgctg ctacttaata agtacaacag atctcgcatt cattgcaaca atatattcac
     7621 ttatatttaa ctacaaaaca ccatttcagt gacattagtt tctactggaa agatgacaga
     7681 gtgatgacag tgatgaaaaa agctgtgtgc tttcagcagg attcgcatca ggcatcagcg
     7741 cacgaacgcc ggatgcggcg tgaacgcctg atccagccta ccaaacccgc accgattgta
     7801 ggcatgataa gacgcgcaag cgtcgcatca ggcatctgcg cacgaacgcc ttatccgcct
     7861 aaacacatta aacccgcaat aacgcgcttc cgagataatc gttcgcgtcc ttcactcccg
     7921 gcagcgaaaa aaaataaccg ccgccgatag gtttaacgta ttcctccagc gcttcgccat
     7981 tgagcctttt ttgtactgtc aggaagcctt tttccagatc gtgttgatag cagacaaaca
     8041 gtagccccat atccagttgt ccggagttgg tgacgcccaa cgaataactg tagccacgac
     8101 gcagcatcag gctggactca ctctctggcg tgcggggatt cgccagccgg atatggctgt
     8161 ccagcgcaat caccttcccc tccggatcgc tggcgtaatc gggaacatca tgctcatgct
     8221 gcattcccag cggcgcaccg gtttgtttgt cgcgaccaaa aatcgtctgc tgttctttca
     8281 gtggcgttct gtcccaaaac tccacccgaa actgaatcaa ccgcaccgcc tggtagctgc
     8341 cgccgattgt ccacgcaggc tcctgctgat ctgccgtcac ccacaccact tgttgcatca
     8401 acttatcatt ctggctatcg ggattggcag tgccgtcttt gaaacccagc aaattaatcg
     8461 gcgtctcttt gcctttacta cgcgctgcat gatcggaaat aaacccttcc cgcttccagc
     8521 gcacgctgag caaatccggc gtgtgtttga tgatatcgcg cagcgcatgg atcaccgtgt
     8581 cctgggtgtt ggcgcaaatt tgtagcaaca catcaccatg acataacgcc gcatccagcg
     8641 agtcgttggg gaaacgcgtc attttctgca actttttcgg catctgcggt gtaaggccaa
     8701 accgctcatc aaacaatgag tggcccaccg ataacgttat ggtgagatta tcggacgcaa
     8761 tgtagccgcc aagaatgccg gaatcgagtg gaggcaggcg cggatttggc gtttctggtg
     8821 ccgctccgcc ctgagtcaga aaagcaaaac gctgagtcaa caagcgaaac aacctttcaa
     8881 gatcggcttt atcgctggca agcacatcaa acgccaccag catcattgcg gcctgttgtg
     8941 gcgtcaggat ccctgcctga tgctcaccat aaaacggctg tttctcattg cgtgcatccg
     9001 gtgaaagcgt gcccggtgca ctttgcgttt tttgtgcatg agcgaccgga caacttcccg
     9061 ccagcgccag cgcaccaatc cctttcagta aacgtcggcg tgacggttca ttcacgccgt
     9121 tttcatcttc atactgcata acgtttaatc cagccccagc acaccgcgaa gttgcgccag
     9181 atcttccgcc agcgcagtaa tcggcccttt cagcgcatta cggtcagcat cggtcaattt
     9241 gtcgtaggtt tcaaaaccgt ctttagtacg gtatttcgcc agaatggtat cgaccttttt
     9301 gaaattggca tcgacttttg ccagcagttc cgggttggcc ttttgcagtt gtggacgcag
     9361 cagatcgaca attttctgcg agccttcaac gttagcctgg aaatcccaca gatcggtgtg
     9421 gctgtagcga tcttcttcac cgctgatttt gctggctgcc acttcctcaa tcagtccggc
     9481 tgcaccgccg accacttttg aaggtgggaa agccagttca ctgatgcgtt tttgcaaatc
     9541 gaccacatcg gtataaagct ggtcggcata cttatccatc cctttagtgg tgttatcgcc
     9601 aaacaatgct ttttccagac ggtggaaacc ggtgaatttt ggatcggcgg ctttttgctc
     9661 gtaatcatct tcacgggcgt caatgctacc atccagatcg gagaacagtt cagcaatcgg
     9721 ttcaatgcgc tcatagtgct ggcgcgtcgg cgcatacagt gcttttgctt tttcgatatc
     9781 gcctgcttta atagcgtcgg taaaggcttt ggtgtcggtc accagctgcg tggtttccgc
     9841 catgacatac gctttatatg cagtaattgc accaccaaga cttaacagcg catcactttg
     9901 cgccgcatcc gccgttgcct cacctttgac gatcaacttc cctttcgggt tagtcagcag
     9961 accgcaggtc atatcgtatt cgccaggctg taaattcgcc gtcattttct ggctaaaacc
    10021 aggggcgata ttttcccgct cttccaccac catcacgcct ttgaggatct cccactccag
    10081 cgccttctgg ctgtggttct gaataatgaa ctgtgttttc ccggcgttaa ccgtaatggt
    10141 catcggttcg cactgcttat ccgtcacggt cactttgacc tgcggcacat cagcggcgtt
    10201 agccataaaa gcagaagaaa acagcgcagc cacgctcaac tgcaatgcgt tacggcggaa
    10261 gttaatggtc atgacgagac tatcccttta aagtatgttg taactaagca agagtttgcg
    10321 tcgtatttgc tacgcagagc gagacgctgt cgcccctgcg cgtggtggca gagcaaatgc
    10381 caccagcgcc gggatgagat aaataaacca gacggcgact tcgctgacac ttggcgcttc
    10441 ctgatagcca aaaatccctt ccatcagcgt gccaaacagc gagtgagttg agagcaccgc
    10501 actcatatcg aaggcgattt cctgaaagtg gttccacaat ccggcttcat gaaatgcgcg
    10561 aatggcacca gccgccagcc ctgcggcgac gaagagaata aacaggctgg tccatttaaa
    10621 aaatgcacca agattgaggc gaataccgcc ccagtagagc aggaagccga gcaccacggc
    10681 tgtagcaaga ccgagcattg cacccagcgg cggccagatc ccgacatctt gttgaaacgc
    10741 cgccagcagg aaaaagaccg actccagccc ctcccttgca acggcaaaaa agaccatcat
    10801 caccagcgcc cagccatgat gatttccacg ctgcaatgcg ctatcgactg cctgctccag
    10861 ttgtactttg acgttgcgcg acactttgcg catccagaaa accatccagg taaggatcac
    10921 cacggcgatc accgccacga taccttcaaa cagttcctgt tctttttgcg gaaattcgcc
    10981 ggtggtttcg ttaatgaaga tacccaagcc caggcacaac gcagcggcaa gcaacacgcc
    11041 aatccacatc acaccaatcc atcggcctcg ctgggtacgc ttaagatagc tggcgatcaa
    11101 actgacaatc agcgcggctt caagtccttc gcgcaacata atgagaaatg gaacaaacat
    11161 gccacccacc cttaaacgtt tttctgagtc aatctatgca aagaaaagta aatcgcagtg
    11221 atagcgatta tcattacggc aaagaaaaat acaagcggaa tgtttataaa atgatgttgg
    11281 aatgtaaaca aagagaggta atgccagggg atataaacga ttattccagg tacaaagatg
    11341 ggattgttta caacgagcca tgtgttcttg ccagatgccg cgtaaacgcc ttatccagcc
    11401 gacaaaactg tccagactca atgcatcaga gcttccatgt aaaactgata agcgggtgta
    11461 tcaagcaatt tcgtccatcc gcgattatca aatctaactt tttgcccgga acataataac
    11521 agctcgggtg ttggtggcgt caaagtcgct gaagcgttcc ccgaaggccg gggcagccca
    11581 catggatgtg ggctgagggc gcgtcttaca ggacattacc tcgcgcccga cccggtagcc
    11641 gaaggggata agctgaaggt accgcgcagc ggcgattttg ttcgccagag cccggggtgc
    11701 agggggtggc ggcgattggc cgccccctgc accattcaat ttatatggcg aggcacatta
    11761 agggagcgca acccgctgct gaagccaaac aaaaccgccg caggctaagt cccttagctt
    11821 tcctgcaacc gtgacggcgg agccgaatga tagtgcgcat cggcctcggc aaagcgtttt
    11881 tgcatcgccg ctgacggcgc tttacccagc aaactaaaca ctacaatccc gatactacca
    11941 aagataaagc ccggaataat ttcgtacaga cccagccagc cgaactgttt ccagacgata
    12001 accgtcagcg caccgatgat catccccgcc agcgcaccgt tgcgcgtcat gcgtgaccac
    12061 atcaccgaga acagcaccac cggaccaaac gccgcgccaa agcctgccca cgcgtagctc
    12121 actaagccca gcacgcggtt ttccgggttt gccgccagcg caatggccac cagcgccacc
    12181 accagcacca tcacacgccc tacccacacc agctctttct ggctggcctg tttacgcaga
    12241 aacgctttgt ataaatcttc ggtaatcgca ctggagcaca ccagcagctg gcaacttaag
    12301 gttgacatta ccgccgccag aatcgccgac agcagaatcc cggcaatcca cgggttaaac
    12361 agaatttgcg ccagttcgat aaacacacgt tcagcattct ggtttaccgc acctgctacc
    12421 gacgggtgct cattaaagta agcaatacca aagaagccga cagccaccgc ccctgccagg
    12481 cagaggatca tccaggtcat actgatacga cgcgcatgga caatgctgtg gtgagaatcc
    12541 gccgccataa aacgcgccag gatgtgcggc tgcccgaagt aacccagccc ccagcccatc
    12601 agcgagataa tggcaacaaa gttcagtcct ttgagcatat cgacgttttc gatgctcttt
    12661 tgtttgatca cttccagcga gtcgccaaac ccaccaacgc taatgattac gataaccggc
    12721 gtcaggatca gggcaaaaat catcaggctg gcctgtacag tgtcagtcca gctcactgca
    12781 aggaagccac caataaaggt gtaaaggatc gtcgccgcag cgcccgccca cagagccgtt
    12841 tcgtagctca tgccaaaggt actttcaaac agacgcgcgc ctgccacgat gcccgaagcg
    12901 caataaatgg tgaagaacag caaaatcacc agcgcggaga taatgcgcaa aatacggctt
    12961 ttatcttcaa agcgcccggt aaaataatcc ggcaatgtta aggcgttatt gttgtattcg
    13021 gtatgcacac gcaaccgccc ggccaccagc ttccagttaa tccacgcgcc taatgtcagg
    13081 ccaatggcga tccagctttc ggaaatcccg gaaagaaaaa ccgcgcccgg caaccccatt
    13141 aacagccagc cgctcatatc cgatgcaccc gccgataatg ccgtcacgaa tggcccaagg
    13201 ctacgtccgc ccagaatata gtcgtcaaag tttttcgttg atcgccaggc gataaaccca
    13261 atcaatatca tgccaaagat atagacacaa aatgtcacca acatcggtgt gctaatagcc
    13321 atctaaagtc tccaaaaaat tattatcggc aatgtcgaaa cttgccgtta tatctgccac
    13381 cggaacgggg tagcagagtt tatgttttac cagggcgacc gtatcctgcc ggaagcgctg
    13441 gttattcaca atcgatttaa cacaccattt acattaaatt ttagtgctca gcgacactat
    13501 ttttcatcag gttgcactct ctcacatttt ttgcggttgc acctttcaaa aatgttaact
    13561 gccgcagaga aaaagtctga gttatttttt taatccctgt catatcgatt tcttttatta
    13621 acatttcatt catttttaag cttgctacgc aggtcacatt taacatggtt gcacaaagtt
    13681 gcaacatcat ggatatttca cgataacgtt aagttgcacc tttcagaaca acaggagtaa
    13741 tggcatggga accaccacca tgggggttaa gctggacgac gcgacgcgtg agcgtattaa
    13801 gtctgccgcg acacgtatcg atcgcacgcc acactggtta attaagcagg cgattttttc
    13861 ttatcttgaa caactggaaa acagcgatac tcttccggag ctacctgcgc tgctctctgg
    13921 cgctgccaat gagagcgatg aagcaccgac tccggcagag gaaccacacc agcctttcct
    13981 cgactttgcc gagcaaattt tgccccagtc ggtttcccgc gccgcgatca ccgctgccta
    14041 tcgccgcccg gaaaccgaag cggtttctat gttgctggaa caagctcgcc tgccgcagcc
    14101 gattgctgaa caggcgcata aactggcata tcaactggcc gataaactgc gtaatcaaaa
    14161 aaatgccagt ggtcgcgcag gtatggtcca ggggttattg caggagtttt cgctgtcatc
    14221 gcaggaaggc gtggcgctga tgtgtctggc agaagcgttg ttgcgtattc ccgacaaagc
    14281 cacccgcgac gcgttaattc gcgacaaaat cagcaacggt aactggcagt cacacattgg
    14341 tcgtagcccg tcgttgtttg ttaatgccgc cacctggggg ctgctgttta ctggcaaact
    14401 ggtttccacc cataacgaag ccagcctctc ccgctcgctg aaccgcatta tcggtaagag
    14461 cggtgaaccg ctgatccgca aaggtgtgga tatggcgatg cgcctgatgg gcgagcagtt
    14521 cgtcactggc gaaaccatcg cggaagcgtt agccaatgcc cgcaagctgg aagagaaagg
    14581 tttccgttac tcttacgaca tgctgggcga agccgcgctg actgccgcag atgcacaggc
    14641 gtatatggtt tcctatcagc aggcgattca cgccattggt aaagcgtcca atggtcgtgg
    14701 catctatgaa ggtccgggca tttcaatcaa gctgtcggcg ctgcatccgc gatacagccg
    14761 cgcccagtat gatcgggtaa tggaagagct ttacccgcgt ctgaaatcac tcaccctgct
    14821 ggcgcgtcag tacgatattg gtattaacat cgatgccgaa gaggccgatc gtctggagat
    14881 ctccctcgat ctgctggaaa aactctgttt cgagccggaa ctggcaggct ggaacggcat
    14941 cggttttgtt attcaggctt atcaaaaacg ctgcccactg gtgattgatt acctgattga
    15001 tctcgccacc cgcagccgtc gccgtctgat gattcgtctg gtgaaaggcg cgtactggga
    15061 tagtgaaatc aaacgtgcgc agatggacgg ccttgaaggt tatccggttt atactcgcaa
    15121 ggtgtatacc gacgtttctt atcttgcctg tgcgaaaaag ctgctggcgg tgccaaatct
    15181 aatctacccg cagttcgcaa cgcataacgc ccatacgctg gcggcaattt atcaactggc
    15241 gggtcagaac tactacccgg gtcagtacga gttccagtgc ctgcacggta tgggcgagcc
    15301 actgtatgag caggtcaccg ggaaagttgc cgacggcaaa cttaaccgtc cgtgtcgtat
    15361 ttatgctcct gtcggtaccc atgaaacgct gctggcgtat ctggtgcgtc gcctgctgga
    15421 aaacggtgct aacacctcgt ttgttaaccg tattgctgac acctctttgc cactggatga
    15481 actggttgcc gatccggtca ctgctgtaga aaaactggcg caacaggaag ggcaaactgg
    15541 attaccacat ccgaaaattc ccctgccgcg cgatctttac ggtcacgggc gcgacaactc
    15601 ggcagggctg gatctcgcta acgaacaccg tctggcatcg ctctcttctg ccctgctcaa
    15661 tagtgcactg caaaaatggc aggccttgcc aatgctggaa caatcggtcg cggctggtga
    15721 gatgtcgccc gttattaacc ctgcggaacc gaaagatatt gtgggctttg tgcgtgaagc
    15781 cacgccgcgt gaagtagaac aggcgctgga aagtgcggtt aataacgcgc caatctggtt
    15841 tgccacgcct ccggctgaac gcgcagcgat tttgcaccgc gctgccgtgc tgatggaaag
    15901 ccagatgcag caactgattg gtattctggt acgtgaggcc ggaaaaacct tcagtaacgc
    15961 cattgccgaa gtgcgcgaag cggtcgattt tctccactac tacgccggac aggtgcggga
    16021 tgatttcgct aacgaaaccc accgtccatt agggcctgtg gtgtgtatca gtccgtggaa
    16081 cttcccgctg gcgattttca ccgggcagat tgctgctgca ctggcggcag gtaacagcgt
    16141 gctggcaaaa ccggcagaac aaacgccgct gattgccgcg caagggatcg ccattttgct
    16201 ggaagcgggt gtaccgccag gcgtggtgca attgctgcca ggtcagggtg aaaccgtggg
    16261 cgcgcaactg acgggtgatg atcgcgtgcg cggggtgatg tttaccggtt caaccgaagt
    16321 cgctacgtta ctgcaacgca atatcgccag ccgcctcgac gctcagggtc gccctattcc
    16381 gctcatcgcg gaaaccggcg gtatgaacgc gatgattgtc gactcctccg ccctgaccga
    16441 acaagtagta atagatgtgc tggcctcagc gttcgacagt gcgggccagc gttgttcggc
    16501 actgcgcgtg ctgtgcctgc aagatgagat tgccgaccac acgttgaaaa tgctgcgcgg
    16561 cgcaatggcc gaatgccgga tgggtaatcc tggtcgcctg accaccgata tcggtccggt
    16621 gattgatagc gaagcgaaag ccaatattga gcgtcatatt cagactatgc gtagtaaagg
    16681 ccgtccggtg ttccaggcag tgcgggaaaa cagtgaagat gcccgtgaat ggcaaagcgg
    16741 cacctttgtc gccccgacgc tgattgaact ggatgacttt gccgaattgc aaaaagaggt
    16801 ctttggtccg gtgctgcatg tggtgcgtta taaccgtaac cagctgccgg agctgatcga
    16861 gcaaattaac gcttccggtt atggtctgac gcttggcgtc catacgcgca ttgatgaaac
    16921 catcgcccag gtcactggct cggcccatgt cggtaacctg tacgttaacc gtaatatggt
    16981 gggcgcagtg gtcggggtgc agccattcgg cggcgaaggg ttgtccggca ccgggccgaa
    17041 agcaggcggt ccgctctatc tctaccgtct gctggcgaat cgcccggaaa gtgcactggc
    17101 agtgacgctc gcgcgtcagg atgcagagta tccggtcgat gcgcagttga aagccgcatt
    17161 gactcagccg ctaaatgcac tgcgggaatg ggcggcaaac cgtccagaat tgcaggcgtt
    17221 atgtacgcaa tatggcgagc tggcgcaggc aggaacacaa cgattgctgc cagggccgac
    17281 gggtgaacgc aacacctgga cgctgctgcc gcgtgagcgc gtgttgtgta ttgcggatga
    17341 tgaacaggat gcgctgactc agctcgccgc cgtgctggcg gtgggcagcc aggtactgtg
    17401 gccggatgac acgctgcatc gtcagttagt gaaggcattg ccatcggcag tcagcgaacg
    17461 tattcaactg gcgaaagcgg aaaatataac cgctcaaccc tttgatgcgg tgatcttcca
    17521 cggtgattcg gatcagcttc gcgcattgtg tgaagccgtt gccgcgcggg atggcgcaat
    17581 tgtttcggtg cagggttttg cccgaggcga aagcaatatc cttctggaac ggctgtatat
    17641 cgaacgctcg ctgagtgtga ataccgctgc cgctggcggt aacgccagtt taatgacgat
    17701 cggttaaaca ggtagccgga ggatgttaca acctcctccg gcatctttaa cgtggtcgaa
    17761 tcccctcaat aataatccgc tgcacgtttt caaccgtttg attgaaaaat acctcatcgc
    17821 gcaacgtcgc gcctgtcacc gcctccacct gaggggcgaa atcggcgtaa tgttgagtgg
    17881 aagcccaaat cataaaaatc aaatgctgcg ggtcaatcgg cgcgagtttg ccgcttttta
    17941 cccaaccggc aatcagcgcc gatttctcat caattaatgc cttcaaatcg cccgtcagtt
    18001 catccattaa cagcggcgcg cctgccagca tctccataca gaacaggcgc gaagcctgcg
    18061 gataatcgcg tgagacttcc agcttcagac ggatgtactc tttgatcgcc gccagcgggg
    18121 cgaaatcttc acgaaacgct tttaacggtg ccagccagat atcgagaatc tgccgcagca
    18181 cggcaatata cagcgcctct tttgacggaa agtaatacag cagattggtt tttgaaacac
    18241 ccgccaactc tgcgatctgc tccagccttg tgccgtgaaa accgaattgt gaaaaagtgt
    18301 ccagtgctgc gctaagaatc gcttttttct tcgcgctcac tgcgcgcgaa cgtttacccg
    18361 ttgttttcac tgcgccttgc gtcatgcgct ctcccctctt gtagattggc aacaggatag
    18421 caaaacccct gcccggcctc gaccttctgc actttcatcg cgcacggatg catgttttat
    18481 gtgcaactgt tttgaccgtt tagtccactt ttttccagat attttagaca acttaacatt
    18541 aacatattaa taacattcac tttttaaaaa ctggcatccg ctttgcaaac aaaccattac
    18601 gcggctgaaa aggatatgca ggatgcagcg ccccgtctga ctttcacttt acgagacgaa
    18661 gagaggttga tgatgaaaat tggcgtattc gtacctattg gcaacaacgg ctggctcatt
    18721 tcgacccacg cgccgcagta catgccgacc tttgaactga ataaagccat tgtgcaaaaa
    18781 gcggagcact accatttcga tttcgccctg tcgatgatca aactgcgtgg ctttggcggc
    18841 aaaactgagt tctgggatca caaccttgag tcgttcacct tgatggcggg gctggcagcc
    18901 gtgacctcgc gcattcagat atacgcgaca gctgccacct taacgttacc tccggcaatc
    18961 gtcgcccgta tggccgcaac cattgactcc atctctggcg ggcgttttgg cgtcaacctc
    19021 gtgactggct ggcaaaagcc cgagtatgag cagatgggta tctggcctgg cgatgactat
    19081 ttctcccgtc gttacgacta tctcaccgaa tatgttcagg tgctgcgcga cctgtggggc
    19141 tcggggaaaa gcgattttaa aggcgatttt ttcactatgg atgattgtcg cgtcagtccg
    19201 caaccgagtg tccccatgaa agtgatctgc gccgggcaaa gcgacgctgg catggcgttc
    19261 tccgcccggt atgccgattt taacttctgt ttcggcaaag gcgtaaatac acccacggct
    19321 ttcgccccga ccgctgcgcg gatgaaacag gccgcagagc aaaccggacg cgacgttggc
    19381 tcttatgtgt tgtttatggt gattgccgat gaaaccgacg atgccgctcg cgccaaatgg
    19441 gaacactaca aagcgggcgc ggatgaagag gcgttaagct ggctaaccga acaaagtcag
    19501 aaagataccc gctccggtac tgacaccaac gtccgtcaga tggccgatcc cacttcggcg
    19561 gtaaacatca atatggggac gttagtcggt tcttacgcca gtgtcgcgcg catgttagat
    19621 gaagtcgcaa gcgtgcctgg tgccgaaggc gtgctattga ccttcgatga ttttctgtcg
    19681 ggaatcgaaa ccttcggcga gcgcattcaa ccactgatgc agtgccgcgc ccatctccct
    19741 gcgctgactc aggaggtggc atgatgacaa tcttaaccgc tcgaccggaa gccattacct
    19801 tcgatccgca gcaaagtgcg ctgattgtgg tggatatgca aaacgcttat gccacgccag
    19861 gcggctactt agatctcgcc gggtttgatg tctcaaccac tcgcccggtc attgccaaca
    19921 ttcaaaccgc cgtgaccgca gcgcgagcgg cagggatgtt gattatctgg tttcaaaatg
    19981 gctgggatga acagtatgtc gaggctggcg gccccggctc accaaatttt cataaatcga
    20041 acgccctgaa aaccatgcgt aagcagccgc agctacaggg gaaattgctg gcgaaaggct
    20101 cctgggatta tcaactggtg gatgaactgg tgccgcagcc tggcgatatt gtgctgccta
    20161 agccgcgcta cagcggtttc ttcaatacgc cgctggacag cattttgcgc agccgcggaa
    20221 tacgccatct ggttttcacc ggtatcgcta ccaacgtctg cgtcgaatcg acgctacgcg
    20281 acggcttttt tctggagtat ttcggcgtgg tgctggaaga cgcaacacac caggcggggc
    20341 cggaatttgc acagaaggcc gcgttgttca atatcgaaac cttttttggc tgggtcagcg
    20401 acgtcgaaac gttctgcgac gcgctttctc ccacgtcctt tgctcgtatc gcttaaggag
    20461 tttaacgatg ccaaaatccg taattattcc cgctggcagc agcgcaccgc tggctccctt
    20521 cgttcccggc acgctggctg atggcgtggt gtatgtctcc ggtacgctgg cttttgatca
    20581 acataataac gtgctgtttg ccgatgaccc aaaggcgcaa acccgccacg ttctggaaac
    20641 catccgcaag gtgatcgaga cggcgggtgg cacgatggcg gatgtgacct tcaacagcat
    20701 ctttattacc gactggaaaa attacgccgc gattaacgaa atctacgccg agtttttccc
    20761 gggtgacaaa ccggcgcgat tctgcattca gtgcggactg gtaaaacctg acgcgctggt
    20821 ggaaatcgcc acaattgcgc atatcgccaa gtgaggccgc gatgaaactt tcactctcac
    20881 ctccccctta tgctgatgcg cccgtagtgg tgttgatttc gggtcttggc ggtagcggca
    20941 gttactggtt accgcaactg gcggtactgg agcaggagta tcaggtaatc tgttacgacc
    21001 agcgcggcac cggcaataat cccgacacgc tggaagaaga ttacagtatc gcccagatgg
    21061 cagcggaact gcatcaggcg ctggtagccg cagggattga gcgttacgcg gtggtcggcc
    21121 atgcgctcgg tgcactggtg ggaatgcagc tggcgctgga ttatcccgcg tcgttaactg
    21181 tgctggtcag cgttaacggc tggctacgaa taaacgccca tacgcgccgc tgttttcagg
    21241 ttcgcgagca gttactacat agcggcggcg cgcaggcatg ggtggaagct cagccattgt
    21301 tcctctatcc cgccgactgg atggcggccc gcgcacctcg cctggaagct gaagacgcgc
    21361 tggcactggc gcattttcag ggcaaaaata atttactgcg tcgacttaac gccctcaaac
    21421 gcgctgactt tagtcaccat gcggatcgca tccgctgccc ggtgcaaatc atctgcgcca
    21481 gtgatgatct gctggtgcca acagcatgtt ccagtgaact tcatgccgcc ctgcccgata
    21541 gccagaaaat ggtgatgcgc tatggcggac acgcctgcaa cgtgaccgat cccgaaacgt
    21601 ttaatgctct gttactcaac gggcttgcca gcctgttaca tcaccgtgaa gccgccctgt
    21661 aaggaattgc tatgaacgaa gccattagcc caggtgcgct tagcaccctg ttcaccgatg
    21721 cccgcactca caacggctgg cgggagacac ccgtcagcga tgagacgtta cgggagcttt
    21781 atgccctgat gaaatggggg ccgacatcag ctaactgttc tccggcacgg atcgtgttta
    21841 tccgcactgc agaaggaaaa gaacgtctgc gcccggcgct ttccagcggc aatctgcaaa
    21901 aaaccctgac cgcgcccgtc accgcgatcg tcgcctggga cagtgaattt tatgaacggt
    21961 taccacagct gtttcctcac ggcgatgccc gcagttggtt tacctccagc ccacaacttg
    22021 ccgaagaaac agcgtttcgc aacagttcca tacaggcggc ctatctgatc gtcgcctgcc
    22081 gggcgctggg gctggatacc ggcccgatgt ccggctttga ccgtcaacac gtggacgacg
    22141 ccttttttac gggcagcacg ctgaagagca atctgctgat taatatcggc tatggcgata
    22201 gcagcaagct ttatgcgcgc ctgccacgtc tgtcctttga agaagcctgc gggctgttgt
    22261 aaggagcaaa ctatgaacat tgtcgatcaa caaacttttc gcgatgcgat gtcctgcatg
    22321 ggcgcggcgg tcaatattat caccacggac ggtccagccg ggcgcgccgg gttcaccgcc
    22381 agcgccgtct gcagcgtgac cgatacgccg cccacattac tggtgtgcct gaatcgtggg
    22441 gcgtccgtct ggccggtatt caatgaaaat cgaacgctgt gtgtaaatac acttagcgcc
    22501 gggcaggagc cgctttcaaa cctttttggc ggcaaaacgc ccatggaaca ccgctttgcc
    22561 gccgcccgct ggcagactgg tgtgaccgga tgcccgcaac tggaagaggc actggtttcg
    22621 tttgactgcc gtatcagcca ggtggtgagc gtcggcaccc acgacattct gttttgtgcc
    22681 atagaagcga ttcatcgtca cgccaccccc tacgggctgg tgtggttcga tcgcagttat
    22741 cacgcgttga tgcgccctgc ttgttaacct ccttaaggag acagctgatg gcaatgttcg
    22801 gttttcctca ctggcagtta aaatcgacct ctacagagag cggcgtggtc gcgccggatg
    22861 aacgattgcc gtttgctcag acggcagtaa tgggcgttca acacgcggtg gcgatgtttg
    22921 gcgcgacggt gttaatgccg attctgatgg ggctggatcc caatctttcc attttgatgt
    22981 cggggatcgg cacgttactg tttttcttta ttaccggcgg gcgcgtcccc agttatctcg
    23041 gttccagcgc ggcttttgtc ggcgtggtga ttgccgcgac gggctttaac ggtcagggca
    23101 ttaacccgaa tatcagcatc gcgctgggcg ggattatcgc ctgtgggctg gtttataccg
    23161 tcattggcct ggtggtgatg aaaatcggta cgcgctggat tgaacggctg atgccgccgg
    23221 tggtgacggg tgcggtagtg atggcgattg gcctgaactt agcgcctatc gcagtgaaaa
    23281 gtgtatcggc ttcggccttt gatagctgga tggcggtaat gacggtgctg tgtattgggc
    23341 tggtagcggt gtttacccgt ggcatgatcc agcggttatt gattctcgta ggattaattg
    23401 tcgcctgtct gctgtatggc gtgatgacca acgttttagg gctgggaaaa gccgtcgatt
    23461 ttactctcgt cagccatgct gcctggttcg gtttgccaca tttttccact cctgctttta
    23521 atggtcaggc aatgatgctg attgcgcccg tggcggtgat tctggtggcg gaaaacttag
    23581 gccatctcaa ggcggtcgcc gggatgaccg gacgcaatat ggatccatat atggggcggg
    23641 cattcgtggg cgatgggctg gcgacgatgc tttccggctc tgtcggcggt agcggcgtga
    23701 ccacctatgc tgaaaatatc ggtgtgatgg ccgtgacaaa agtctactcg acgctggtgt
    23761 ttgttgctgc tgccgtcatc gccatgctgt tgggcttttc gccgaaattt ggcgcgctga
    23821 ttcataccat tccagcaccg gtgattggtg gtgcatcaat tgttgtgttc gggctgattg
    23881 ccgtcgcagg cgcaaggatc tgggtacaaa accgtgtcga tttaagccag aacggtaatt
    23941 tgattatggt cgcagtaacc ttagtgctgg gcgcgggtga ttttgctctc acgctaggcg
    24001 gttttacgtt gggagggatc ggtacagcaa cctttggcgc gattttactc aatgcgttgt
    24061 taagcagaag gttggttgac gttccaccgc cagaagttgt tcatcaggag ccataaacag
    24121 aaaaccgccg gggtaaccgg cggtgatgac tttttgacga gcttcagaca aaaatacatt
    24181 cgtcattgat tagcccggct agttgtcgct tttgccgtga ctgctcttgc cacctttttt
    24241 acctgcttca gatgcgcgct gcgggtcatt tttgaaatta cccccgctgt gctgtccacc
    24301 ttttttacct gcttctgatg ctctttcgcg gtcttctgca aaattgccgg aaccgcctcg
    24361 atggtttgcc attactgacc tccgtctggg tatagacatc atattgcata gaattaacgc
    24421 ggcgtatggc ggctgccata ttttcgctac gtgggactaa gcttagtgaa ctgacacggt
    24481 caggcccgca tttagtaata tcctgcaaca gcttcttagc gagaatagat atcatctcca
    24541 ataattatcc ataagccgct cttatgatta aaaaaacgcg atgagataaa aatgtgtcat
    24601 tttgcgacaa aattacgcgc ttgtgaaagt tgttataaat caaataagtg gttgtgaaat
    24661 ttgcactctg aaaaggacgt cttatcttta aaagagtggt agcgaatcgc tacggaatag
    24721 agataacacg aggagtggtt agaaatggct aaagttctgg tgctttatta ttccatgtac
    24781 ggacatatcg aaacgatggc acgcgcagtc gctgagggtg caagcaaagt ggatggcgca
    24841 gaagttgtcg ttaagcgtgt accggaaacc atgccgccgc aattatttga aaaagcaggc
    24901 ggtaaaacgc aaactgcacc ggttgcaacc ccgcaagaac tggccgatta cgacgccatt
    24961 atttttggta cacctacccg ctttggcaac atgtccggtc aaatgcgtac cttcctcgac
    25021 cagacgggcg gcctgtgggc ttccggcgca ctatacggaa aactggcgag cgtctttagt
    25081 tccaccggta ctggcggcgg tcaggaacaa accatcacat ccacctggac gacccttgcg
    25141 catcacggca tggtgattgt ccccattggc tacgcagcgc aggaattatt tgacgtttca
    25201 caggttcgcg gcggtacgcc gtacggcgca accaccatcg caggcggtga cggttcacgc
    25261 cagccaagcc aggaagaact gtctattgct cgttatcaag gggaatatgt cgcaggtctg
    25321 gcagttaaac ttaacggcta atcttcaaca ggaggatacg catgccaact caagaagcga
    25381 aagctcatca cgtcggtgaa tgggcatctc tgcgcaatac gtcgccggaa atagccgagg
    25441 ccattttcga agtcgccggg tacgacgaaa aaatggcgga aaagatttgg gaagaaggta
    25501 gcgatgaagt gttagtcaaa gcctttgcta agaccgataa agattcgctt ttttggggcg
    25561 aacagaccat cgaacgtaaa aacgtttaat acgcgactcc cccgcttctc gcgggggagt
    25621 tttctgttat ttcaccgctt cattcaacac gctatcaaac ttatccattg ggcagaaacc
    25681 gttggcatct atcggacaac cgcttaattc cagcgtcacg cgctgcgcag gtgcctgaag
    25741 ggttaacgcg tcagcattac gtaactgttc tgcactctgg tacacatact cgattttcat
    25801 caggtcgcga ttggctttgc tgtcatgcca acgctggaaa acgattttgc cgccaatcgg
    25861 cgtgcgttcg ttctggtcat gcaactgata cggtttgaaa tccagcgccg ttaacagaga
    25921 ggcaatgttg gagtcgtgcc caactaacac cgtgattttc ggtgcactgg tgcgatcggt
    25981 gaccagggct ttgtcgatat aactgaccag cggtttcgca acattgcgcg ccacttccgg
    26041 tgaggtaaac aggctgtcct ggtaaccgtt tttaagcttc gacaacacct tccactgctg
    26101 gtcagatttg atttctcccc aggccacctg atccatcgga aaaccttcgt agtattgcaa
    26161 agtaaacgca tctaccagcg agttgccgac tttcagcggc ccggaaacac ctggttcttg
    26221 ttgatacttc gcgctaaagg tatttttgcc atccaccagc gaacattgtt gtttctcttt
    26281 acaggcaggg gaatctttat agttaacgat tttttccagt agctggtagc tgtcggtaag
    26341 ctggagtttg ctgagctctt tctccattgc cgccaccgcc tgttcactga atgcggcgga
    26401 atcatcggtg atcactgggt taaaggttgg gtccatagtg cccatttttt cctgatgatg
    26461 aacaggaata tcacacccgg ggaatgcgcc ggtaataaag aactgtgcag tcgcaacggt
    26521 acgttgcaga ctattggcat aggcatatac ggtgtccggc ggcgggcatt cccccgattt
    26581 taccatcccc tgctctgcca gccattcacg catgtaatgg cccatataca cttcgagcac
    26641 gccacctttg gtggtgagtt gcccaccggg gacgtcccat tctggccatt tattcggcgt
    26701 cgactgctcc agcacactgc cattgttcgc cagcggcgca cgtaagttat ggcggctcat
    26761 catgagcact tgctgtagct gatagccttc cggtacggtt tgtgcctgag catttgaagc
    26821 gagtaaaact atccctgcca cagttgcggc gattagcgtt ttgttcattc ctggcacctc
    26881 ttttgttatc aatcactaaa gagtgtgaca gaaatatgac ttttttccgg gaacaggttc
    26941 gcaaaatggc ttgttgaaaa cctgtttttc tatatacatc ttcaaaaaca ggcaaggttt
    27001 gacgcaaact atgcctgatt agcccctgcc tcgcgtacgc cgctgataat tgcacctgtg
    27061 gctaaacaga atatctgcgg cgtttccaat acagagacaa tatttaccaa taaaaaagtg
    27121 tgagactaat aacaatggtt ttagcatatg cctttgccat taaattgcat aacgatttaa
    27181 atagtcaagt tgcgcattca tttccttatt gtttaacttg taagcctgag caaaaagttc
    27241 aggagggagg taaacgtcat aatgattgcc ggtatttatt aaataaccat attcaagatc
    27301 ttcatctgtt gaaaaggatt catttcccaa ggagattgat gccacattac tattaacaat
    27361 aaataatgag caaatgctat ctgaaagaac agcaagaaaa ttcttcaccg ctttaatcct
    27421 atccgacgtg tcggtatcta aagcaagcaa attcttgtta tcattaaata taaactcggg
    27481 taaatgcagg atacattttt cgttattcaa gcaactatca atgaattctt ttttaaaaaa
    27541 c
//
LOCUS       DACBVI010000051        27474 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.051, whole genome shotgun sequence.
ACCESSION   DACBVI010000051 DACBVI010000000
VERSION     DACBVI010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.051"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(47..1297)
                     /locus_tag="IU325_10565"
     CDS             complement(47..1297)
                     /locus_tag="IU325_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HAP5809465.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWMKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMDGAKLLKCSEFGDAIIKNM"
     gene            1879..2328
                     /locus_tag="IU325_10570"
     CDS             1879..2328
                     /locus_tag="IU325_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809466.1"
                     /translation="MDTTEERNGTYFYRGHPNLSKIELLNVIFLEEFCKELGIDSVSG
                     AAILSGQPWLGTRAKPQGATLGTSVVSKYARILMRDARMPFGISVHTPVGVRMQKSNK
                     LAAIIARWVPWIGWASLSMSLYRVSTRTQEKYNLIAKPQDRIKWTSF"
     gene            2328..2663
                     /locus_tag="IU325_10575"
     CDS             2328..2663
                     /locus_tag="IU325_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1493 family protein"
                     /protein_id="HAP5809467.1"
                     /translation="MATTITDEVLALFRDEIPGYQDDHGKEIPLELDSDLFDYPRDDL
                     EDAIRKYKERFGINLDNLDWSLYLPWESAPLLQRWFKLKREEVETTRLPLTVRMFAES
                     AEAGKWLYQ"
     gene            2996..3634
                     /gene="rluE"
                     /locus_tag="IU325_10580"
     CDS             2996..3634
                     /gene="rluE"
                     /locus_tag="IU325_10580"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HAP5809468.1"
                     /translation="MKYFSDSKSSFRKKQVSRLNSQHSTKRKPENQPTRVILFNKPYD
                     VLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPGKRTGK
                     IYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGVELVDEPAWLWPRNPPIRERKSIPT
                     SWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREATD"
     gene            3644..4105
                     /gene="nudJ"
                     /locus_tag="IU325_10585"
     CDS             3644..4105
                     /gene="nudJ"
                     /locus_tag="IU325_10585"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HAP5809469.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEANETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSRQRYPLEMIGDFNWPFTKGVI"
     gene            4159..5265
                     /gene="mnmA"
                     /locus_tag="IU325_10590"
     CDS             4159..5265
                     /gene="mnmA"
                     /locus_tag="IU325_10590"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP5809470.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKDGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            5301..5942
                     /gene="hflD"
                     /locus_tag="IU325_10595"
     CDS             5301..5942
                     /gene="hflD"
                     /locus_tag="IU325_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HAP5809471.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            5946..7316
                     /gene="purB"
                     /locus_tag="IU325_10600"
     CDS             5946..7316
                     /gene="purB"
                     /locus_tag="IU325_10600"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP5809472.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            7485..8156
                     /gene="phoP"
                     /locus_tag="IU325_10605"
     CDS             7485..8156
                     /gene="phoP"
                     /locus_tag="IU325_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HAP5809473.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            8156..9616
                     /gene="phoQ"
                     /locus_tag="IU325_10610"
     CDS             8156..9616
                     /gene="phoQ"
                     /locus_tag="IU325_10610"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HAP5809474.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            9692..10813
                     /locus_tag="IU325_10615"
     CDS             9692..10813
                     /locus_tag="IU325_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP5809475.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(10862..12088)
                     /gene="pepT"
                     /locus_tag="IU325_10620"
     CDS             complement(10862..12088)
                     /gene="pepT"
                     /locus_tag="IU325_10620"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP5809476.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            12338..13474
                     /gene="potA"
                     /locus_tag="IU325_10625"
     CDS             12338..13474
                     /gene="potA"
                     /locus_tag="IU325_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HAP5809477.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            13458..14315
                     /gene="potB"
                     /locus_tag="IU325_10630"
     CDS             13458..14315
                     /gene="potB"
                     /locus_tag="IU325_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HAP5809478.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            14312..15106
                     /gene="potC"
                     /locus_tag="IU325_10635"
     CDS             14312..15106
                     /gene="potC"
                     /locus_tag="IU325_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HAP5809479.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            15103..16149
                     /gene="potD"
                     /locus_tag="IU325_10640"
     CDS             15103..16149
                     /gene="potD"
                     /locus_tag="IU325_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HAP5809480.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            16207..16668
                     /locus_tag="IU325_10645"
     CDS             16207..16668
                     /locus_tag="IU325_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HAP5809481.1"
                     /translation="MSQDSRVFFHIFLGIGLVLILISVVVFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKNGSWYPVVAFRPTPDYTLIFNSSIGSDFYEDSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFISVGLLISMPSSKKTRRKRKSRP"
     gene            16665..17450
                     /locus_tag="IU325_10650"
     CDS             16665..17450
                     /locus_tag="IU325_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP5809482.1"
                     /translation="MKKIIILLSLLILLPLTATSNPLIPIMKTLFTDVTGTVPDAEEI
                     AHKAELFRQQTGIAPFIVVVPGVNEASFRQNGKAMLAHASSSLSDVKGSVLLLFTTRE
                     PRLIMITNGQVESGLDDKHLGLLVENHTQAYLHADLWYQGINNALAVLQAQILKQPTP
                     PLTYYPHPGQQQENDPPGSTTILGLFAWAVAFIVFTAFFYYTTRLNYALKFAVAMTIA
                     NMGYQALCLYIDNSLAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(17588..18409)
                     /gene="cobB"
                     /locus_tag="IU325_10655"
     CDS             complement(17588..18409)
                     /gene="cobB"
                     /locus_tag="IU325_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP5809483.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(18425..19336)
                     /gene="nagK"
                     /locus_tag="IU325_10660"
     CDS             complement(18425..19336)
                     /gene="nagK"
                     /locus_tag="IU325_10660"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HAP5809484.1"
                     /translation="MYYGFDIGGTKIALGVFDSVRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADKRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(19365..20609)
                     /gene="lolE"
                     /locus_tag="IU325_10665"
     CDS             complement(19365..20609)
                     /gene="lolE"
                     /locus_tag="IU325_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HAP5809485.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDKVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(20609..21310)
                     /gene="lolD"
                     /locus_tag="IU325_10670"
     CDS             complement(20609..21310)
                     /gene="lolD"
                     /locus_tag="IU325_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HAP5809486.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(21303..22502)
                     /gene="lolC"
                     /locus_tag="IU325_10675"
     CDS             complement(21303..22502)
                     /gene="lolC"
                     /locus_tag="IU325_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HAP5809487.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            22763..23836
                     /locus_tag="IU325_10680"
     CDS             22763..23836
                     /locus_tag="IU325_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAP5809488.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLSTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATLMTYVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGTN
                     TIAIYTTHRILVEIFSLTLLAPMNAARWSPQVELTLLMVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            23964..27410
                     /locus_tag="IU325_10685"
     CDS             23964..27410
                     /locus_tag="IU325_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP5809489.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRAINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSVKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
BASE COUNT         6777 a         7057 c         6924 g         6716 t
ORIGIN      
        1 aacgggcgta taacacgccc gtttcaatat ttaacacatg tagagattac atgttcttga
       61 tgatcgcatc accaaactct gaacatttca gcagtttagc gccatccatc agacgctcga
      121 agtcataggt tacagtcttg gcgttgattg cgccttccat acctttaaca atcaggtctg
      181 cggcttcagt ccagcccata tggcgcagca tcatctcagc ggagagaata atagagcctg
      241 ggtttacttt gtcctgaccg gcatatttcg gcgcagtacc gtgggtggct tcaaacaggg
      301 cgcattcgtc accgatgttt gcgcctgggg cgataccgat accgccgacc tgtgcagcca
      361 gagcgtcaga aatgtagtca ccgttcaggt tcatacaggc gataacatca tattcagccg
      421 gacgcagcag gatctgttgc aggaatgcgt cagcaatcac gtctttaatg acgatctctt
      481 tgccggtatt cgggttttta actttcatcc acgggccgcc gtcgatcagt tcaccgccaa
      541 actcttcacg cgccagctgg tagccccagt ctttaaacgc gccttcggtg aacttcatga
      601 tgttgccttt gtgcaccagg gtcacagagt cacgatcgtt agcaattgcg tattcgatcg
      661 ctgcacgaac cagacgtttg gtgccttctt ccgaacacgg cttaataccg ataccgcaat
      721 gttccgggaa gcgaattttc ttcacgccca tctcttcacg caggaattta atcactttct
      781 cggcgtcggc agagtcagct ttccattcga tacccgcata aatgtcttcc gagttttcac
      841 ggaagataac catatcggtc agttcaggat gtttaaccgg gcttggggtg ccctgatagt
      901 aacgtaccgg acgcaggcag atgtagagat ccagttcctg gcgcagggca acgttcagag
      961 aacgaatacc gccaccaacc ggagtggtca gagggccttt aatggcaacg cgatattcac
     1021 gaatcagatc aagggtttca gcaggcagcc agacatcctg accataaacc tgtgtggatt
     1081 tttcaccggt gtaaatttcc atccaggaga ttttacgctc gcctttatag gctttctcga
     1141 ctgcagcgtc gaccactttc agcatggctg gggttacatc tacaccgatt ccatcacctt
     1201 caatgtaagg gataatcgga ttttcaggaa cgttgagttt gccgttttgc agggtgatct
     1261 tcttgccttg tgccggaaca actactttac tttccattca cctctccttc gagcgctact
     1321 ggtttgctcg tctgccacca cgttgcatat gcgtttgcgt cctgcgatac ggatgcatta
     1381 gagcaatttt ttgttaatga tttgtaattg gcttgtcaat actaccctat tgtttgccgt
     1441 catgaaagat gcgcgttatt aggctataat gcggcaattc ataatctctg aaaataccat
     1501 gcaaaaaact tcttttagac caaagacagt atttttcaga ccttcaaata aggcattaaa
     1561 tatcaataag ttacaccagt ttttaagcaa taaaaaaaac caccagagat aactaacagg
     1621 atgctatttg gtcaaaattt gacattactc gactcagcga ctatctcact ttgagcacta
     1681 ttgggaaaat tatcaaaagt catggttgaa gcgacaagca acgagtaaat tatggcatcc
     1741 cccctttttg aggttcacat gaaacgcttt gcactgatag ctgtgaaact cgttgccttt
     1801 actggttctg catctgctgc aaccgaagaa atcaacttca agtaacagaa acactatata
     1861 acaatggagt gttgacaaat ggatactacc gaagaacgca atggaacata tttctacaga
     1921 ggacacccga acctgtccaa aatagaacta ctcaacgtca tattccttga agaattctgt
     1981 aaggaattgg ggattgattc ggtatcaggt gcagcaatct tatctggtca accgtggctg
     2041 ggtacaagag cgaagccgca aggggcaacc ctgggaacaa gtgttgtctc aaagtatgcg
     2101 cgtatactga tgagagatgc acgcatgcct tttggtattt ctgttcatac accagtgggt
     2161 gtaaggatgc agaaatctaa taagcttgct gcaattatcg ctcgttgggt cccgtggatt
     2221 ggttgggctt cactgtcgat gagtctttac agggtttcga ctcgtaccca ggaaaaatac
     2281 aatctgattg ccaaacctca agaccgtatt aagtggactt ccttctaatg gcgacaacaa
     2341 tcacagacga agttttagca ctgttccgtg atgagattcc aggttatcag gatgaccacg
     2401 ggaaggaaat tccgttagaa ctggactctg atttgttcga ctatccacga gatgatttag
     2461 aggacgctat acggaaatac aaagagaggt ttgggattaa cctggataat ctggactggt
     2521 ctttgtacct tccatgggag tctgcaccat tgcttcaacg atggttcaag ctcaagcggg
     2581 aagaggtaga aacaacacgt ttaccgttaa ctgtacggat gtttgcagaa tcagcagaag
     2641 cgggaaaatg gctgtatcag tagaataaaa aagaaggctg cgatgcccct cttattactg
     2701 ctgatgatgg tagtggtaaa gcatcatcct gaacttgaac agtgaagtgc tgtcacccat
     2761 cagtaataca gaaacactat ttttcatacc agtatttatt caattcatca atgtaaaatg
     2821 tgttcattgg gccatattta tcaccatgca gccacacaac cgctgctcac taacccaggt
     2881 attgatgaaa ccgcgctgaa gagcctgaac cctgaacctc taacgagtac cttaaaagtc
     2941 caatgcaatc cgacactgtg ctaccctctg cgtatagctc tatttggaat ttaccatgaa
     3001 atactttagc gattctaaat cttcttttag aaaaaaacag gttagtcgac taaactcgca
     3061 acactccact aagcgtaaac ctgaaaacca gcccacgcgc gtgatcctgt tcaataaacc
     3121 ctacgatgtt cttccgcagt tcaccgatga agccggacgc aaaacattaa aagaattcat
     3181 cccggttcag ggcgtttatg cagcaggtcg tcttgaccgc gatagcgaag ggttactggt
     3241 gctgaccaat aacggcgcgt tgcaggcgcg tttaacccag ccgggtaaac gcaccggaaa
     3301 aatctattat gtgcaggtgg aaggtattcc cacacaagac gcacttgaag ccttgcgcaa
     3361 tggcgtaacc ttaaatgatg gccctaccct gcccgcaggc gtagagctgg ttgacgaacc
     3421 cgcgtggtta tggccgcgga atccaccaat tcgtgaacgc aaaagtattc ccaccagctg
     3481 gctgaagatc accttatatg aaggacgtaa tcgccaggtg cgccgcatga ccgcccatgt
     3541 tggtttcccc acgctgcgac tgattcgcta tgcgatgggt gattactctt tggataatct
     3601 tgccaacggc gaatggcgag aagcgacaga ttaaggacgc aaaatgttta aaccgcacgt
     3661 taccgttgct tgcgtggtgc acgcagaagg caaattttta gtcgttgaag agacgattaa
     3721 tggtaaagcg ttatggaacc aacctgccgg gcatctggaa gccaatgaaa ccttagtgga
     3781 agccgccgcc cgtgagctgt gggaagaaac cggcatcagc gcgcagccgc aacactttat
     3841 tcgtatgcat cagtggattg cgccagataa aacgccgttt ttgcgtttcc tgtttgctat
     3901 tgagcttgag caaatatgcc cgacccagcc tcatgacagc gatatcgact gctgccgttg
     3961 ggttagcgcc gaagaaattt tacaggcgtc aaatctgcgt tcgccgctgg tggcggaaag
     4021 tattcgttgt tatcaaagca ggcaacgcta tccgctagag atgattggcg attttaactg
     4081 gccttttaca aagggtgtca tctaaaaggc tgcgtgatag aatacgccgc cttgaagttc
     4141 aatgtcgtga gtgatccaat gtctgaaacc gcaaaaaaag taatcgtcgg catgtccggc
     4201 ggtgtcgatt cctccgtttc tgcctggctg ttgcaacaac agggatatca ggtcgaaggc
     4261 ctgtttatga agaactggga agaagacgac ggtgaggaat attgcacagc ggcagcggat
     4321 ctggctgatg cccaggctgt ctgcgacaag ctcggcattg aactgcacac cgttaacttt
     4381 gctgccgagt actgggacaa cgtcttcgaa ctgttccttg ccgaatataa agccggtcgc
     4441 acgccgaatc cggatattct gtgcaacaaa gagatcaaat ttaaagcctt cctcgaattt
     4501 gccgctgaag atttaggtgc cgattatatc gctaccggtc attacgtacg tcgggcagat
     4561 gtcgatggca agagccgcct gctgcgtggt ctggacagca ataaagacca gagctacttc
     4621 ctttatacgc tcagccatga gcagattgcg caaagcctgt tcccggtcgg cgaactggaa
     4681 aaaccgcagg tgcgtaagat tgctgaagat ctcggtctgg tcaccgcgaa gaaaaaagac
     4741 tctaccggca tttgcttcat tggcgagcgt aaattccgcg agttcctggg ccgttatctc
     4801 ccggcgcaac cgggcaaaat cattaccgtc gatggcgatg aaattggtga gcaccagggg
     4861 ctgatgtatc acaccctcgg tcagcgtaaa ggtctgggta tcggtggcac caaagacggc
     4921 accgaagaac cgtggtatgt ggtcgataaa gacgtcgaaa acaacattct gattgttgcg
     4981 cagggccatg aacacccgcg tctgatgtct gtcggtctga ttgcccagca gctgcactgg
     5041 gtcgatcgcg agccgttcac cggtactatg cgttgcacag taaaaacccg ctatcgccag
     5101 accgacatcc cgtgcaccgt caaggcgctg gacgatgatc gcattgaagt gattttcgat
     5161 gagccggttg ccgccgtgac gccgggccag tctgccgttt tctataacgg tgaagtgtgc
     5221 ctcggtggcg gtattattga gcagcgtctg ccgctgccgg tctgattatt atctttactt
     5281 aaaacaagga agcagtgaac gtggcaaaga attactatga catcaccctc gccctagccg
     5341 gtatttgtca gtcggcacgc ctggtgcaac aactcgctca ccaggggcat tgtgatgccg
     5401 atgcgctaca cgtctcactc aacagtatta ttgatatgaa ccccagctcg acgctggcgg
     5461 tttttggcgg cagcgaagcc aacctgcgcg tcgggctgga aaccttgctc ggcgtgctca
     5521 atgccagcag tcgccagggc ttaaacgctg agttaacccg ctacacactc agcctgatgg
     5581 tgcttgagcg caaactctcc tcagcgaaag gtgcgctcga cactctgggc aaccggatca
     5641 acggcctgca acgccagctc gaacacttcg atttacagtc cgaaacgctg atgagcgcga
     5701 tggctgctat ctatgttgat gtgattagcc cgcttggccc gcgcattcag gtaaccggtt
     5761 cccctgctgt actgcaaagc ccacaagtgc aggcgaaagt tcgcgcaacc ctgctggcag
     5821 gcattcgcgc cgccgtgctc tggcaccagg ttggcggcgg acgtctgcaa ctgatgtttt
     5881 ctcgtaatcg cctgaccact caggcaaaac aaattcttgc tcatttaacc ccggagttgt
     5941 gatctatgga attatcctca ctgaccgccg tttcccctgt cgatggacgc tacggcgata
     6001 aagtcagcgc gctgcgcggg attttcagcg aatatggttt gctgaaattc cgtgtacaag
     6061 ttgaagtacg ttggctgcaa aaactggccg cgcacgcagc gatcaaggaa gttcctgctt
     6121 ttgctgccga cgcaatcggt taccttgatg caattgtcgc cagtttcagc gaagaagatg
     6181 ccgcacgcat caaaaccatc gagcgtacca ctaaccacga cgttaaagcg gttgagtatt
     6241 tcctgaaaga aaaagtggcg gagatcccgg aactgcacgc ggtttctgaa ttcatccact
     6301 ttgcctgtac ttcggaagat atcaataacc tctcccacgc attaatgctg aaaaccgcgc
     6361 gtgatgaagt gatcctgccg tactggcgtc aactgattga cggcattaaa gatctcgccg
     6421 ttcagtatcg tgatatcccg ctgctgtctc gtacccacgg tcagccagcc acgccgtcaa
     6481 ccatcggtaa agagatggca aacgtcgcct accgtatgga gcgccagtac cgccagctta
     6541 atcaggtgga aattctcggc aaaatcaacg gcgcggtcgg taactataac gcccacatcg
     6601 ccgcttaccc ggaagttgac tggcatcagt tcagcgaaga gttcgtcacc tcgctgggta
     6661 ttcagtggaa cccgtacact acccagatcg aaccgcacga ctacatcgcc gagctgtttg
     6721 attgcgttgc gcgcttcaac accattctga tcgactttga tcgtgacgtc tggggttata
     6781 tcgcccttaa ccacttcaaa cagaaaacta ttgctggtga gattggttct tccaccatgc
     6841 cgcataaagt taacccgatc gacttcgaaa actccgaagg gaacctgggc ctttccaacg
     6901 cggtgttgca gcacctggca agcaaactgc cagtttcccg ctggcagcgt gacctgaccg
     6961 actccaccgt gctgcgtaac ctcggcgtgg gtatcggtta tgcgctgatt gcgtatcaat
     7021 ccaccttgaa aggcgtgagc aaactggaag tgaaccgtga ccatctgctg gatgaactgg
     7081 atcacaactg ggaagtgctg gctgagccaa tccagacagt tatgcgtcgc tatggcatcg
     7141 aaaaaccgta cgagaagctg aaagagctga ctcgcggtaa gcgcgttgac gccgaaggca
     7201 tgaagcagtt tatcgacggt ctggcgttgc cagaagaaga gaaagcccgc ctgaaagcaa
     7261 tgacgccggc taactacatt ggtcgcgcca tcaccatggt tgatgagctg aaataagcct
     7321 caaaacagtg ccggatggcg atgctgtccg gcctgcttat taagattatc cactttttct
     7381 tttttcactt tacctcccct ccccgctggt ttatttaatg tttaccccca taaccacata
     7441 atcgcgttac actattttaa taattaagac agggagaaat aaaaatgcgc gtactggttg
     7501 ttgaagacaa tgcgttgtta cgtcaccacc ttaaagttca gattcaggat gctggtcatc
     7561 aggtcgatga cgcagaagat gccaaagaag ccgattatta tctcaatgaa catttaccgg
     7621 atattgcgat tgtcgatctg ggattgccag acgaggacgg tctgtcactg attcgccgct
     7681 ggcgtagcaa tgatgtttca ctgccgattc tggtattaac cgcccgtgaa agctggcagg
     7741 acaaagtcga agtattaagt gctggcgctg atgattacgt gactaaacca tttcatattg
     7801 aagaggtgat ggcgcgaatg caggcattaa tgcggcgtaa tagcggtctg gcttcacagg
     7861 tcatttcgct acccccgttt caggttgatc tctctcgccg tgaattatct attaatgacg
     7921 aagtgatcaa actgaccgcg ttcgaataca ccattatgga aacgttgata cgcaataatg
     7981 gcaaagtggt cagcaaagat tcgttaatgc tacaactcta cccggatgcg gagctgcggg
     8041 aaagtcatac catagatgta ctgatgggac gtctgcgcaa aaaaatacag gcacaatatc
     8101 cgcaagaagt gattaccacc gttcgcggcc agggctatct gttcgaattg cgctgatgaa
     8161 aaaattactg cgtctttttt tcccgctctc gctgcgggta cgttttctgt tagcaacggc
     8221 agcagtagta ttggtgcttt cgcttgccta cggaatggtt gcgctgatcg gttatagcgt
     8281 cagtttcgat aaaaccacgt ttcggctgtt acgtggcgag agcaatctgt tctataccct
     8341 tgcgaagtgg gaaaacaata agttgcatgt cgagttaccc gaaaatatcg acaagcaaag
     8401 ccccaccatg acgctaattt atgatgagaa cgggcagctt ttatgggcgc aacgtgacgt
     8461 gccctggctg atgaagatga tccagcctga ctggctgaaa tcgaatggtt ttcatgaaat
     8521 tgaagcggat gttaacgata ccagcctctt gctgagtgga gatcattcga tacagcaaca
     8581 gttgcaggaa gtgcgggaag atgatgacga cgcggagatg acccactcgg tggcggtaaa
     8641 cgtctacccg gcaacatcgc ggatgccaaa gttgaccatt gtggtggtgg ataccattcc
     8701 ggtggagcta aaaagttcct atatggtctg gagctggttt atctatgtgc tctcagccaa
     8761 tctgctgtta gtgatcccgc tgctgtgggt cgccgcctgg tggagtttac gccccatcga
     8821 agccctggca aaagaagtcc gcgaactgga agaacataac cgcgaattgc tcaatccagc
     8881 cacaacgcga gaactgacca gtctggtacg aaacctgaac cgattgttaa aaagtgaacg
     8941 cgaacgttac gacaaatatc gtacgacgct caccgacctg acccatagtc tgaaaacgcc
     9001 actggcggtg ctgcaaagta cgctgcgttc tctgcgtagt gagaagatga gcgtcagtga
     9061 tgctgaaccg gtaatgctgg agcaaatcag ccgcatttca cagcaaattg gctactacct
     9121 gcatcgtgcc agtatgcgcg gtgggacatt gctcagccgc gagctgcatc cggtcgcccc
     9181 actgctggac aatctcacct cggcgctgaa caaagtgtat caacgcaaag gggtcaatat
     9241 ctctctcgat atttcgccag agatcagctt tgtcggtgag cagaacgatt ttgtcgaggt
     9301 gatgggcaac gtgctggata atgcctgtaa atattgcctc gagtttgtcg aaatatctgc
     9361 aaggcaaaca gacgagcatc tctatattgt ggtcgaggat gatggccccg gtattccatt
     9421 aagcaagcga gaggtcattt tcgaccgtgg tcaacgggtt gatactttac gccctgggca
     9481 aggtgtaggg ctggcggtag cccgcgaaat caccgagcaa tatgagggta aaatcgtcgc
     9541 cggagagagc atgctgggcg gtgcgcggat ggaggtgatt tttggtcgcc agcattctgc
     9601 gccgaaagat gaataaatat gtccatactt cacgcattac gttaagcatc cgttataatc
     9661 ggttgcagat accagcctgt ggatgcttaa catggaatac caactcactc ttaactggcc
     9721 cgattttctt gaacgtcact ggcagaaacg tccggtggtg ttaaaacgcg gctttaataa
     9781 ttttattgac ccgatctctc cagacgagtt ggcgggtttg gcgatggaaa gcgaagtcga
     9841 cagtcgactg gtcagtcacc aggatggcaa atggcaggtc agccacggcc cgttcgaaag
     9901 ctacgatcat ctcggtgaaa ctaactggtc attgttagtg caggcagtga accactggca
     9961 tgagccgacc gccgcgctga tgcgaccgtt ccgtgaacta ccggactggc gtattgatga
    10021 tctgatgatc tctttttctg tacctggcgg cggcgtcggc ccgcatctcg atcagtacga
    10081 cgtgtttatc attcagggta ccggacgtcg tcgctggcga gtgggcgaaa agctgcaaat
    10141 gaaacagcac tgcccacatc cggatctgtt acaggtcgat ccgttcgaag ccatcatcga
    10201 tgaagagctg gagcctggcg atatccttta tattccgcca ggattcccgc atgaaggcta
    10261 cgcgctggaa aatgcgatga actattccgt gggttttcgc gcgccaaata cgcgggaatt
    10321 aattagcgga tttgccgatt atgtgctgca acgtgaactg ggcggcaact actacagcga
    10381 tccggatgtt ccacctcgcg ctcatcctgc ggacgttctg ccgcaagaga tggataaact
    10441 gcgtgagatg atgctcgaat tgatcaacca gccggaacac tttaagcaat ggtttggcga
    10501 gtttatatcc cagtcacgtc atgaactgga tatcgcgccg ccagagccgc cttatcagcc
    10561 ggatgaaatc tacgatgcgc taaaacaagg tgatgtgctg gtgcgcctgg gtggtctgcg
    10621 cgtattgcgc attggcgacg acgtgtatgc caatggtgag aagatcgatt ccccgcaccg
    10681 tccggcactg gatgcgctcg ccagcaacat tgcgctgacc gcggagaatt ttggtgatgc
    10741 actggaagat ccgtcattcc tcgcgatgct cgcagcgctg gtcaatagcg ggtattggtt
    10801 cttcgaaggg taagtttgaa gttaagctgg atgcggcatc acatgccgca tcccttttcg
    10861 cttacttccg ttgcgccgtc aactcggcaa tacggacgat cacctgcacc gctttttcca
    10921 taccttccag agtcacaaac tcatgcttac catgatagtt gtaaccgcca gtgaacaggt
    10981 tcgggcacgg taatcccata aacgacaact gcgcgccgtc ggtaccgccg cggatcggtt
    11041 tcagttccgg ttcaatatcg cagtcacgca tcgcctgctg ggcgatatcg agaatatgcg
    11101 gatgctcaac cactttctcg cgcatattgt agtaactgtc ttcaatcacc aattcaatgt
    11161 agcaatcagg atgtaaccct ttgcccactt ttttggcgat ctccatcatt ttacgtttac
    11221 gcgcttcaaa ctgtttacgg tcgaaatcac ggatgatgta gtgcatatcg gcccgttcaa
    11281 cggtgccttt catgctcgcc aggtgataga aaccttcata gccttctgtc atttccgggc
    11341 tttcatccgc cggaacttcc gcatgaatac gtgccgccag cgacagcgca ttcaccatta
    11401 cacctttcgc tgtgcctggg tgaacgttat tgcctacaat tttgatattg accgacgcgg
    11461 cgttgaagtt ttcaaactcc agttcgccta cgccaccacc atcaacggtg taagcccagc
    11521 gggcatcgaa ggcgtcaaca tcaaaatgtt tcgccccttt gcccacttct tcatccgggg
    11581 taaaggcgac gcgaatatca ccatgcggaa tgtttttctg ttgcaatacc gccagcgcgg
    11641 tcatgatttc tgcaatacct gctttgtcat cggcacctaa caaggtttta ccatcggtgg
    11701 taatcagcgt ctgacccagt agctgatgca gcaccgggaa cataaccggt gataaaactt
    11761 catcgccgat acccagcgca atatcgccac cgcgatagtt ttcaacaatt tgcggattca
    11821 catttttgcc gctgcaatcc ggtgaggtat ccacatgaga aataaagcca atcgccggga
    11881 tatcgccagg gacgttagcc ggtaacgtcg ccatcaaagt gcccttctca cttaaggtca
    11941 cattgataag ccccatctct tcgagctgct ctttcagcag atgcaataac ttccattggc
    12001 cttccgtgct gggaacctgt ctcacccctg cttttgattg ggtatccaga gacacgtagt
    12061 tcaaaaatcg ctcaagtagt ttatccatgt agtcaccctc actttttgtg acaacattat
    12121 taagaagcaa gaaaagacaa atattgcgtc aggtcacttt tacccctgca agcgggaata
    12181 tttatcagca ttaccttaat gaatataaag ctaagccagt aattcacaac aaagattggc
    12241 gattcaagcg gtttgccgta gaattaccgc cctcattaag agtcaccaag gtggttaacc
    12301 acaaaccccg catcggtaag ccatccgttg cgtttacatg ggacagagta aaaaattgaa
    12361 taaacaaccg agttcgcttt caccgctggt gcaattggcg ggaattcgca aatgctttga
    12421 tggtaaagag gtcattcccc agctggatct gactatcaac aatggcgagt tcctcacgct
    12481 gcttggccct tctggctgcg gtaaaacaac cgttcttcgc ctgattgcag gtctggaaac
    12541 tgttgattcc ggacgcatca tgctggataa cgaggacatc acccacgttc cggcggaaaa
    12601 ccgctatgtg aatactgttt tccaaagcta cgcacttttc ccccacatga ccgtgttcga
    12661 aaatgtggcc tttgggttgc gcatgcaaaa aacccccgct gctgaaatta cgccccgcgt
    12721 gatggaagcc ctgcggatgg tgcagctgga aaccttcgct caacgcaaac cgcatcagct
    12781 ctctggtggt caacagcaac gcgtcgccat tgcccgcgcg gtggttaaca agcctcgtct
    12841 gttgttgctg gatgagtcgc tctcagcact ggattacaaa ctgcgcaagc aaatgcagaa
    12901 cgagctgaaa gcgttacagc gtaagcttgg cattacattc gtcttcgtga ctcacgacca
    12961 ggaagaagca ctcaccatgt cggacaggat tgtggtgatg cgcgatggtc gcattgagca
    13021 agacggcacg ccgcgtgaaa tctatgaaga gccgaaaaac ctgtttgttg ctgggtttat
    13081 tggcgaaatc aatatgttta acgccactgt catcgaacgt ctcgacgagc agcgcgtacg
    13141 cgccaacgtt gaaggccgcg aatgtaatat ctacgttaac ttcgccgttg aaccggggca
    13201 aaaactgcat gttctgctgc gcccggaaga cttacgtgtt gaagagatta acgacgacaa
    13261 ccacgctgaa gggctgattg gttacgttcg cgagcgtaac tacaaaggca tgacgctgga
    13321 gtcggttgtt gaactggaaa atggcaagat ggtgatggtc agcgaattct tcaatgaaga
    13381 cgatcctgac tttgaccact ctctcgacca aaaaatggcc attaattggg tagaaagctg
    13441 ggaggtcgta ctggctgatg aagaacacaa gtaagttcca gaatgtagtg attgtcacta
    13501 ttgtcggttg gcttgtgttg tttgtctttc tgcccaacct gatgatcatt ggcaccagct
    13561 ttttgacccg cgacgacgcc agtttcgtca aaatggtctt tacgctggat aactacacgc
    13621 gtctgctcga tccgctctat tttgaagtgc tattgcactc gctgaatatg gcgctgatcg
    13681 ccaccctcgc ctgcctggtg ctgggctatc cttttgcctg gtttctggcg aagctgccac
    13741 acaaggtgcg tccgctgctg ctgtttctgc tgattgttcc gttctggacc aactcattga
    13801 ttcgtatcta cgggctgaaa attttcctca gcaccaaagg ctatctcaac gagtttttgc
    13861 tctggctggg cgttattgat acaccaattc gcatcatgtt cacgcccagt gcggtgatta
    13921 tcggtctggt ttacattctg ctaccgttta tggtgatgcc gctgtactcc agtatcgaaa
    13981 aactggataa gccgttgctt gaggcggcgc gcgatctcgg tgccagcaag ttgcagacct
    14041 ttatccgcat cattatccca ctgacgatgc cgggaattat tgccggatgt ctgctggtga
    14101 tgctgccagc gatgggcctg ttctatgtgt ccgacctgat gggcggtgcg aaaaacctgc
    14161 tgatcggtaa cgtcatcaag gttcagttcc tcaatattcg tgactggccg tttggtgcag
    14221 ccaccagcat tacgctgact atcgtaatgg gcctgatgtt gctggtttac tggcgcgctt
    14281 ctcgtctgct gaacaaaaag gtggaactcg aatgatcggt cgactgcttc gcggcggttt
    14341 tatgaccgct atctacgcgt acctgtatat cccaatcatt attttgattg tgaactcctt
    14401 taacagctcg cgctttggca tcaactggca gggttttacc accaaatggt atagcctgct
    14461 gatgaacaac gacagcctgt tacaggcagc gcagcattca ctaacaatgg cggtgttttc
    14521 ggcgacgttt gctacgctta tcggttcact gacggcagtt gcgctgtacc gttatcgctt
    14581 tcgtggtaag ccgttcgtta gcggaatgct gtttgtggtg atgatgtcac cagatatcgt
    14641 gatggcgatt tcgctgctgg tgctgtttat gctgctgggt attcagcttg gcttctggtc
    14701 gctgctgttc tcgcatatca ccttctgcct gccatttgtg gtggtgacgg tgtactcgcg
    14761 cctgaaaggt tttgacgtgc gaatgctgga agcggcgaaa gatctcggtg ccagcgaatt
    14821 taccattctg cgaaaaatca ttctgccact ggcaatgcca gcggtggcgg cgggctgggt
    14881 gttaagcttt accctgtcga tggacgatgt ggtggtttct tcgttcgtca ccggaccaag
    14941 ttatgaaatt ctgccgttaa aaatttattc gatggttaaa gtcggcgtat cgccggaagt
    15001 taacgcgctg gcaaccatat tactggtgct gtcgctggtg atggtaattg ccagccagct
    15061 tattgctcgt gataaaacga aaggtaacac aggggacgtt aaatgaaaaa atggtcacgc
    15121 cacctgctcg cggcgggtgc tctggcactg ggcatgagcg ccgctcacgc cgatgacaac
    15181 aacacgctgt atttctacaa ctggaccgag tacgtgccgc caggactgct tgaacagttc
    15241 accaaagaaa ccggtattaa ggttatctat tcgacttacg agtcgaacga aaccatgtac
    15301 gcgaagctga aaacgtacaa agacggtgcc tatgacctgg tggttccttc aacctattac
    15361 gtcgataaaa tgcgtaaaga agggatgatc cagaagatcg acaagtcgaa gttaagcaac
    15421 ttcagcaacc tcgatccaga catgctcaac aagccgtttg acccgaataa cgactactcc
    15481 attccgtata tctggggggc gacggcgatt ggcgttaacg gtgatgcggt agatccgaaa
    15541 tccgttacca gctgggccga tctgtggaag cctgagtaca aaggcagcct gctgctgacc
    15601 gacgatgccc gtgaagtgtt ccagatggcg ctgcgcaaac tgggctactc cggtaacact
    15661 accgatccga aagagattga agctgcatat aacgagttga aaaaactgat gccaaacgtc
    15721 gcggcgttta actccgataa ccccgctaac ccgtacatgg aaggcgaagt taacctcggc
    15781 atgatctgga acggttctgc tttcgttgca cgccaggcgg gtacgccaat tgatgtggtg
    15841 tggccgaaag aaggcggcat tttctggatg gacagcttgg cgatcccggc aaatgccaaa
    15901 aacaaagaag gcgcgctgaa attgatcaac ttcctgctgc gcccagatgt ggcaaaacag
    15961 gttgcagaaa ctatcggtta tccaacgcca aaccttgcgg cgcgtaagct gttaagtccg
    16021 gaagtggcga acgataaaac gctctacccg gatgctgaaa ccattaaaaa tggtgaatgg
    16081 cagaatgacg ttggcgcagc cagcagcatt tatgaagagt attatcagaa gctgaaagca
    16141 ggacgttaat tcagcaagcg ggcggtggta ttaccgcccg ttttttattt agcaaggatg
    16201 tggctaatga gtcaggacag tagagttttc tttcatatat tcctcggtat cggtctggta
    16261 ctaatcctca tttctgttgt tgtcttttat aaccagttca cctatagcaa agatgcgatt
    16321 catactgaag gcgtcattgt tgataccgtc tggcactcca gtcattctca tcgcacgggt
    16381 aaaaatggtt cgtggtatcc cgtcgttgcc tttcgaccga cgcctgatta cacgctaatt
    16441 ttcaattcga gtattggcag cgatttttat gaagacagcg aaggcgataa agttaatgtt
    16501 tattattctc caggacatcc tgaaaaggct gagataaata acccgtgggt taattttttt
    16561 aaatgggggt ttatcggcat tatgggcgtt atttttatct ccgtcggttt attaatcagt
    16621 atgccctcct ctaaaaagac acgcaggaaa cggaagtccc gcccatgaaa aaaatcataa
    16681 tattgctcag tttactgata ctcctgcctc tgacggcaac cagtaatcca ttgattccaa
    16741 taatgaagac tctttttaca gatgttacag gtactgtacc ggatgctgaa gagatcgcgc
    16801 ataaagccga gctttttcgc caacaaaccg ggattgcgcc gtttatcgtg gttgtacctg
    16861 gcgttaatga agcaagcttc aggcaaaatg gcaaagcgat gctggcgcat gcgtcatctt
    16921 cattgagtga tgtaaaaggc agcgtcctgt tactttttac cacccgcgag ccacggttaa
    16981 ttatgataac caacggccag gttgaaagcg gtctggacga taaacatctc ggccttctgg
    17041 tagaaaatca cactcaggct tatttacatg cggacctctg gtatcaggga atcaataatg
    17101 cattggctgt tttacaagca cagatattaa aacaaccgac gccgccactg acatattatc
    17161 cgcatcccgg acaacagcag gagaatgatc ctcccggcag taccacgata ttaggtcttt
    17221 ttgcctgggc agttgcattc atcgtcttta ctgcattttt ttactacacg acgcgcctta
    17281 attatgcatt aaagtttgca gtggcgatga ctattgccaa tatgggttat caggctttat
    17341 gtttgtacat cgataatagc ttggcgatta ccagaatatc gccgctctgg gcagggctga
    17401 ttggtgtctg tacgtttatt gccgcgttgt tattgacgag taaacggtaa aagaggcagt
    17461 aaagtgtgcg tttattatgc cggatgcggc gcgaacgcct tatctggccc acaaaacccg
    17521 caaattcaat taattgcgtc cccttgtagg cctgataagc gtagcgcatc aggcaatgct
    17581 tcctgcttta taagcccttc agcaactttt caacaaactc tggcaccacc tggcttgccg
    17641 ggccgtaata tttctcggca aattcattac cgacctggct tggttcaaga ttcagttcca
    17701 cggtgtgtgc gccatgcagt ttcgcttcgt gaacaaaccc agccgccgga taaacatgcc
    17761 ctgatgtacc aatggcaatg aaaatatcgg ccattgacaa cgccatataa atttcatcca
    17821 tgccgagtgg catttcgcca aaccaaacga cgtgcgggcg caagggtgcc gggaactggc
    17881 aacaatggca tttatcttct ggcgtaacgt ctccggtcca gtcgagaacc tgaccacttt
    17941 gtgaacaacg aactttcagc agttccccat gcatatgaat aacattggtg ttaccggcac
    18001 gctcatgaag gttgtcgata ttctgcgtca ccagcaaaaa gcgatcgccg agggcatctt
    18061 gcagtttagc cagcgcaaga tgcgcggcgt taggctgaat ttctggctgc tgtagctgtc
    18121 gacgacgggc gttataaaac gcttgcacca gttcaggatc gcgatcgaaa ccttccggcg
    18181 ttgccacatc ttcaacccga tgttcttccc acaggccatc tgcggcgcga aaggtacgaa
    18241 tacctgattc cgcagaaata cctgcccctg tcagtacgag tactcttggt ttttccattg
    18301 cttccggcac cactttatct ctgaaaaaaa tacgctgacg caaacgctcg cgcagacggc
    18361 gtttattttt acgaaaacga cttaaccgat gaccccgacg cgacagcata gcaacctctg
    18421 ttgtttaatc ggttagatgt aggaaggccg caccacgcat tcctcccgca tcaccgtggc
    18481 gcgcgcgttc aatgcgcgga acacgagcta caggtaagag atgacgaggc agcctgtccg
    18541 ccagttgcgt tgtgattgcc gggaaattcg ataagccacc accaatgacg accaggtcag
    18601 ggtcaacaat ggtcaggata tttcccagac aaaccgctaa taaatccaga taacgctcaa
    18661 cgtgcgccct tgcctgctca tcaccttgat cataaagcgc aataatttcg ggagcctgca
    18721 acggttgatg ataatagtgt tgatacagcc acgcaaaacc gcgaccagac agataatttt
    18781 caatgcagcc atgctgacca cagccgcagc ggcgtaacgg gaaatccagc cccatcatgg
    18841 ttaacgcatc aaccggcaga cgcatatggc caaactcgcc ggtaatatag cttttaccgg
    18901 taattggttt accgttgaaa atcagcccgc cgccaacgcc ggtgccgaga atcaacccca
    18961 tcaccagtgg atattgagta aattcatcat cccaggcttc tgaaagggca aaacagttgg
    19021 catcgttatc aaggcggaca tcgcgatcaa gacgtgcgct caggtcggca cgcagcggtt
    19081 taccgctagc agcggggaca ttggcagcat acagcgtacc atcttctgtt tccggcattc
    19141 ccggaatacc gatgccgaca gaacctttac agccaaaacg cttatcggct tcagccacca
    19201 gctcgcacac tgcatctaaa aatgcgtcat agctgtcacg cggtgtcggc acccgctttt
    19261 cccactgcaa ctgccgaacg ctatcaaaca cgccaagcgc aattttcgtt ccaccaatat
    19321 caaacccgta atacattgcc gctccttgtt ttaatgtact gcctttactg gccgctaagg
    19381 actcgcgcag ggtcaatatt actggcgcgc cgcgccggat accaacttgc caaaagactc
    19441 agcaacaatg ctgtgaccag tacgtagaag acgtccagcc agtgcaattc cgatggcagg
    19501 aagtcaataa aatagatatc gctggagagg aactgatgac cgatcaactt ttcaatccac
    19561 tcaataatcg gggtaagttg cagtgaaaca actacgccga taatcacacc acacaggcta
    19621 ccgaatagcc ctgccagcaa tccataccag acaaagatgg cgcgaattaa accatctttc
    19681 gcccccagcg ttcttaatac tgcgatatcg ccactcttgt ctttcaccgc catcactaag
    19741 gtggagacga tgttgaaaca ggccacgcca atcaccagta ccatcgccag atacataatg
    19801 gcgcggatca tctggatatc gcgatacata tagccgtaag taccaatcca acttttaata
    19861 taaacatagc tgttggtcac ttcacccgca tcgcgtacca gcttattggc gttgaaaaca
    19921 tccgtcattt taagggcaat acctgacacg ctggaaccca tatcaagata ttgttgggca
    19981 tctgccagcg ggatcatggc aaaactgtga tcgagttgac cactcaactg caaaataccg
    20041 gcaatgtgca aacgcacacg ttttggctgc atcagtttat gctcgggatt cgagttgggg
    20101 atcataatcg acacccaatc gccctgcttc actttcagcg catccgccac gcctttgccg
    20161 atgataattt gctgttcgcc cgctttaaaa ttgcgccagg catccccctg aacaaacgag
    20221 ggtaatgcgc tcagacgctg ttcctgttgc gggttaacgc cctttacctg gattgcgcga
    20281 agattcgcgc cactttctac cagcccggtg aaattgatat acggcgcagc ggcggcaata
    20341 cccggcactt tctgcacttt atccagtgct tcctgccagt tagtccacgg ctgatccacc
    20401 gcttcgattt caccatgcgg caccaccgcc agaatgcggt tattcagttc gcgttcaaag
    20461 ccgttcatcg cgcttaagcc gacgatcaat accgccacgc cgagggcaat gccaatggta
    20521 gaaatgacgg agatcagcga caccatgccg ccgcgccgcc gtccgcgact aaaacgcagg
    20581 ccaattaata acgataaagg catcgccatt actccgcccc catcaggctc agttccgccg
    20641 tcagacgacc atcacgcatc tccagttggc ggctcatacg tttcgccagt tgcaggtcgt
    20701 gagtaaccac caggaaggcg gtgccctgca aacgattcaa ctccccaagt aactggaaga
    20761 tgctgtcggc gttacgcgca tcaaggttac cggtaggttc atccgccagt accagacgcg
    20821 ggttattgac cagcgcacgg gcaatcgcca cacgctggcg ttcgccacca gaaagttcag
    20881 atgggcggtg attcgcacga tgctccagcc ccaccgcttt taacatctcc agcgcacggc
    20941 tgttgatttc ggcgggcttt ttcttgccaa tcagcagtgg catagccacg ttttccaggg
    21001 cagtaaaatc cggcagcagg tggtgaaact gataaataaa gcccagcttc tggttgcgca
    21061 gttctgcttt cgccgctgac gacagtttgc tcatcggctg accgttaaag atcacatcgc
    21121 cggaggttgg cgtatccagc ccgcccagca ggtgcagcaa ggtgctttta ccggaaccag
    21181 agctaccgac gatcgccatc atttcacctt cgccgacgct gaaactgaca ttgtgcagta
    21241 catcagtttg cacactgcct tcctgatagc gtttgcacag gttgtcgcat tgcaacagga
    21301 tcttattcat aacgtaaagc ctcagcgggt tgtgtggcgg cagcgcgcca tgaagggtaa
    21361 agcgtagaca gcagcgcgat agccatcgcc accagcgcaa taacaatgac ctgtaaaggt
    21421 tcgatagcga ccggcagcgc cgcgccatca agcaggacgc cgattatcgg catcagatta
    21481 tttaactggc tggcaagcag tgcgccaagc gccgctccga ggattgcacc gataatcccg
    21541 gcgctggccc cttgcaccat aaagaccatc atgatttgcc gcggagtcag cccctgagtc
    21601 tgaagaatgg ccacttcgcc ctgcttctcc atcaccatta accccagcga ggtaataatg
    21661 ttaaacgccg caacggcgac aatcaggctc agcagcaagc ccatcatatt tttttccatg
    21721 cgtacggcct ggaacagctc gcctttacgg tcacgccagt cctgccattt gctgccttca
    21781 ggcagttttt gttgacttaa tgagtcgact ttcagcggct catccagcca caatcgccag
    21841 ccggtaatat tgcctgccgg ataacgcatc agacgcgagg catcctcaat attcaccagc
    21901 atttcatagc catcgacttc actgttggca gcgaaagtgc caatcacatt gaacagacgc
    21961 tggcttggaa tacgccccat cggcgtgaac tggctggcag atggcaccat cacgcggatt
    22021 tgatcaccgc gattgacgcc caactgtgag gcaagctgtt caccgaggat gacattatat
    22081 ttccccggct cgaggtcagt ttgtttcaca ttgaccagat acggcgttag tggatctttt
    22141 tgcgccggat cgatacccag catcaccccg accgccacgc tgcgcgcgct ttgcagtacc
    22201 acatcaccgg tagtaatagg tgcgacgcga ttaacgccgt ccagtttgac tgccgtttcc
    22261 gggagttgct gcgggttaag agagccatgc tcagaagaga gaattgcctg tggcatcagg
    22321 ccaaggatgt tgttttgcag ctcgcgctca aaaccgttca tcactgacaa tactgtgacc
    22381 agcgccatca ccccgagggt aatgccgatg gtagaaagcc aggagacgaa acgaccgaag
    22441 cgatccgctg cacgcccacg catgtaacgc aggccaataa atagagcgac aggttggtac
    22501 atgaaatccg tctggttgct gtagcaaaga cacgagtata taagcgaaag cgggatgggt
    22561 aaacgtctga actgtaagtg atcgtcaggg ctaatctgaa aagtagcagc aaatcaatcg
    22621 actaaagggc ctcattttct cattggcctt gtggcctgat ctttatctga aaaataacca
    22681 aatacagaca gatacgcatt acaccacgcg actggcccct caggatcaac agcatgttaa
    22741 ataaaaagag cgtgttgtcc tgatgaaaca aaaagagcta tggattaacc agatcaaagg
    22801 attatgtatt tgtctggtag tgatttatca ctcggtgatt accttttatc cgcatctgag
    22861 cacttttcag catccgttat cggaagtcct gagcaaatgc tggatctatt tcaatcttta
    22921 ccttgcaccc tttcgtatgc cggttttttt ctttatatcc ggctatttga ttcgccgcta
    22981 tatcgacagc gtaccgtggg gaaattgtct cgataaacgg atctggaaca tcttctgggt
    23041 gctggcgctt tggggcgtgg tgcagtggct ggcgttaagt gcgctgaacc agtggctggc
    23101 acctgagcgc gatttaagca atgcctccaa tgccgcttat gccgattcca ccggtgagtt
    23161 cctgcacggg atgatcaccg ccagcaccag cttgtggtat ctgtatgcgt taattgtcta
    23221 tttcgtggta tgtaaaattt ttagccgtct ggcgctgcca ctattcgcct tgtttgtact
    23281 gctgagtgtg gcggttaatt tcgttcccac gccgtggtgg ggaatgaaca gtgtgatccg
    23341 caatttgcct tattacagcc ttggcgcatg gtttggcgca acattaatga cctacgttaa
    23401 agaggtgccg ttgcgccgcc atctgctgat ggcttctttg ctgaccgttc tggcggtcgg
    23461 tgcctggttg tttactatct cgctgctgtt gtcgctggta tcgattgtgg tgatcatgaa
    23521 gctgttttat caatacgagc aacgtttcgg tatgcgctcc accagcctgc tgaatgtgat
    23581 tggcaccaac accattgcta tctacaccac ccatcgcatt ctggtagaga tattcagctt
    23641 aactctgctt gcgccaatga acgcggcacg ctggtcgccg caagtcgaac tgacactcct
    23701 gatggtttac ccctttgtca gtttgttcat ctgtactgtt gcgggcttgc tggtaagaaa
    23761 actttcacag cgtgcattca gcgatctgtt gttctccccg ccttcactgc ccgctgccgt
    23821 cagttactcc cgctaagcca tcgccccagc cggggcgatt caatttgcta aaccatgtcg
    23881 attacggata atgaacagca ttgcttatca gagatatgcc cccatatgtt gaggcatatc
    23941 ctaacgagaa tctgacaacc gttatgcctg aacaatatcg ttatacgctg cccgtcaaag
    24001 cgggtgagca gcgtctgctg ggcgagttaa ctggcgctgc ctgcgcgacg ctggtagcgg
    24061 aaattgccga acgtcacgcc ggaccagtgg tgcttattgc gccagatatg caaaatgctc
    24121 tgcgtttgca tgatgaaatt agccagttta ccgaccagat ggtgatgaat ctggcggact
    24181 gggaaactct tccctacgac agtttttcgc ctcatcagga cattatctcc tcgcgccttt
    24241 ccacccttta ccagctaccg acgatgcaac gtggtgtact gattgttccg gtgaatacgc
    24301 ttatgcagcg cgtttgcccg cacagttttc tccacggcca tgcgctggtg atgaaaaaag
    24361 gtcagcgcct gtcacgagat gcattacgaa cccaactgga cagcgccggt tatcgccatg
    24421 ttgaccaggt tatggagcac ggcgaatacg ccacgcgcgg cgcgttgctg gatctcttcc
    24481 cgatgggaag tgagctgcct tatcgtcttg atttctttga tgatgaaatc gacagtctgc
    24541 gggtgtttga cgtcgacagc cagcgcacgc tggaggaagt agaagcgatc aatctgctgc
    24601 ccgcgcacga atttccgacc gataaagcgg caattgaact gttccgcagc cagtggcgcg
    24661 ataccttcga agtgaagcgt gatccggaac atatttacca gcaagtgagt aaaggcacat
    24721 tacctgcggg gatcgagtac tggcagccat tgttcttcag cgaaccactg ccgccgctgt
    24781 tcagttattt ccctgccaat accttgctgg tgaatactgg cgatctggaa aacagtgccg
    24841 aacgtttcca ggctgacacg ctggcgcgtt ttgagaatcg cggcgtcgat ccgatgcgcc
    24901 cactgttgcc accacaatcg ctctggctgc gggtggacga gctcttctca gagctgaaaa
    24961 actggccccg agtgcagcta aaaactgaac atttaccgac aaaagccgcg aatgccaatt
    25021 taggtttcca gaaactgcca gacctggccg ttcaggcaca acaaaaagcg ccgctggatg
    25081 cgctgcgtaa gttcctcgag tctttcgacg gtccggtggt gttctcggta gaaagtgaag
    25141 gtcgccgtga agcgctgggt gaactgctcg cgcgaattaa aattgctccg caacgcatta
    25201 tgcgtcttga tgaagccagc gaccgtgggc gttatctgat gattggcgct gccgaacatg
    25261 gttttgtcga tacggtgcgt aatctggcgc tgatctgcga aagcgatctg ctcggtgaac
    25321 gcgttgcccg tcgtcgtcag gattctcgcc gcgccatcaa ccccgataca ctgatccgta
    25381 acctcgcgga gctgcatatt ggtcagccgg tggtccatct ggagcacggt gtcggtcgtt
    25441 atgcaggaat gaccacgctg gaagctggag gcattactgg cgagtacctg atgctcacct
    25501 atgccaacga cgccaaactg tatgttccgg tatcgtcact gcatctgatt agccgttacg
    25561 caggtggcgc ggaagaaaac gccccgctgc ataaacttgg cggcgatgcg tggtcacgcg
    25621 cgcggcagaa agcggcggaa aaagtgcgtg atgtggcggc agaattactg gatatttacg
    25681 cgcaacgcgc cgccaaagag ggcttcgcgt ttaaacacga tcgcgagcag tatcagttgt
    25741 tctgcgacag cttcccgttt gaaaccacgc cggatcaggc acaggccatt aatgcggtac
    25801 ttagcgacat gtgtcagccg ctggcaatgg atcgtctggt gtgcggcgat gttggctttg
    25861 gtaaaacaga agtggcgatg cgcgccgctt tcctggcagt agataaccac aagcaggtgg
    25921 cggtgctggt gcctaccacc cttctcgcgc agcagcatta cgacaacttc cgcgaccgtt
    25981 tcgccaactg gccggtacgt atcgaaatga tctcccgttt ccgcagcgtc aaagagcaga
    26041 cgcaaatcct tgcggaagtg gcggaaggga aaatcgatat tctgatcggt acgcacaaac
    26101 tgctgcaaag tgacgtcaag tttaaagatt taggcctgct gattgtcgat gaagaacacc
    26161 gcttcggggt gcgtcataaa gagcgcatta aagcgatgcg tgcgaacgtg gatattctga
    26221 cgcttaccgc tacgccgatc ccacgtacgc tgaatatggc gatgagcgga atgcgcgatc
    26281 tgtcgattat tgccacgccg cccgcccgtc gtctggcggt caaaacgttt gtccgcgagt
    26341 atgacagtct ggtggtccgg gaggcgatcc tgcgtgaaat tttgcgcgga ggacaggttt
    26401 attatctcta caatgatgtg gaaaacatcc agaaagccgc cgaacggctg gcagaactgg
    26461 tacctgaagc gcggattgcc atcggtcacg ggcagatgcg tgagcgcgaa ctggaacggg
    26521 tgatgaatga tttccatcat caacgtttca acgtgctggt ttgtaccacc attatcgaaa
    26581 ccgggatcga catcccgaca gccaacacca ttatcattga acgcgcggat cacttcggtc
    26641 tggcgcagct gcaccagtta cgcggtcgcg tcggacgttc gcatcatcag gcatatgcat
    26701 ggttgctgac accgcatcca aaagcgatga ctaccgatgc acaaaaacgt cttgaagcga
    26761 ttgcctcgct ggaagatctc ggggcaggtt ttgcgctggc aacgcacgat ctggagattc
    26821 gcggcgcggg tgaactgctt ggcgaagaac aaagcggctc aatggaaacc atcggtttct
    26881 cgctgtatat ggagttgctg gaaaacgccg tcgatgcgct gaaagccgga cgcgagccgt
    26941 cgctggaaga tctcaccagt caacaaacag aagtcgagct gcggatgccg tcgctgttgc
    27001 cggatgattt cattcctgac gtgaatacgc gtttgtcgtt ctataaacgt attgccagcg
    27061 ccaaaacgga aaacgaactg gaagagatca aagttgagct tatcgatcgc ttcggcctgc
    27121 tgccggatcc ggcgcgtacc ctgctggata ttgcccgtct gcgccagcaa gcgcagaaac
    27181 tggggatcag gaagctggaa ggtaatgaga aaggcggcgt gatcgaattt gccgagaaga
    27241 atcacgttaa tccggcatgg ctgattggtt tgctgcaaaa acagccgcag cattatcgcc
    27301 tcgatggccc gactcgcctg aagtttattc aggatttgag cgagcgaaaa acgcgtatcg
    27361 aatgggtacg ccagtttatg cgtgaactgg aagagaacgc gatcgcttaa tagtaaatcg
    27421 ggggcgtttc tgcgccccca tctgcaacat ttacaaattc tttgcacttc cctg
//
LOCUS       DACBVI010000052        27017 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.052, whole genome shotgun sequence.
ACCESSION   DACBVI010000052 DACBVI010000000
VERSION     DACBVI010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.052"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..138
                     /locus_tag="IU325_10690"
     CDS             <1..138
                     /locus_tag="IU325_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HAP5809490.1"
                     /translation="GFGATASSFGVLNDIAKYLMCIAMILGRLEIYPVIILFSGFFWR
                     S"
     gene            276..1064
                     /locus_tag="IU325_10695"
     CDS             276..1064
                     /locus_tag="IU325_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HAP5809491.1"
                     /translation="MSEKLKIVYRPLQELSPYAHNARTHSTEQVAQLVESIKQFGWTN
                     PVLIDEKGEIIAGHGRVMAAEMLKMDSVPVIVLSGLTDEQKKAYRLADNRLPMNAGWD
                     EDLLRMELSDLINADFDVSLTGFGPTEIDELLTDVLPGTGNKEEPYTTKIDTPVYEPS
                     GGKPDISELYDDTKTQELISRIRSASLDPDIEKFLLCAAERHTVFNFSRIADYYAHAP
                     AEIQCLFEESALVIIDYQQAIENGFVRMTQRMVAIMHSGEEEYA"
     gene            1057..1989
                     /locus_tag="IU325_10700"
     CDS             1057..1989
                     /locus_tag="IU325_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809492.1"
                     /translation="MRDDFCAFILTHGRPDKVLTYRTLRRAGYTGKIFIVVDDEDKTR
                     HQYMAEFGEQVLVFSKADIASRFDEADNFGDRRSIFYARNACFDLAKLVGCKYFIQLD
                     DDYHEFQFRVDRNYDQAYFPIRKLDAILSEMLAYYESIPALSIAMSQGGDFLGDNGGH
                     ASWVKRKAMNSFICSVDRPFSFMGRINEDVNTYTNLGRCGELFMTIGAVQLGQKQTQK
                     NSGGMTELYLDSGTYVKSFYSVMYAPSCVKISLMGASHKRIHHQVTWNNAAVKILHEK
                     YRKKTPCISMGVTNDSVFESRVSGSVPDDCTTNR"
     gene            1967..2176
                     /locus_tag="IU325_10705"
     CDS             1967..2176
                     /locus_tag="IU325_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809493.1"
                     /translation="MTAQQIADVLDVDLNRLKENREAMTDFYAAIRKGRAKGEAELRA
                     ALFKLARKGDAFALRELLRVDKNQD"
     gene            2180..3274
                     /locus_tag="IU325_10710"
     CDS             2180..3274
                     /locus_tag="IU325_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HAP5809494.1"
                     /translation="MSRPDWGALQQEYIAEYTRSGISPVAWCEARGLNYATARRYIKK
                     TPKNAQKEMRKTAQKSARKKTAQTAQRQNGKSQKKKAVSDALLIEGDTEEISFCPDEF
                     GISDQQAKFAMLVAQGKKPTEAYRLAGYEGQGATANSNASRMLRNARVYRAISYFRNQ
                     YQKRHTADLDLLVSQLMAIVQADPNQLAQFRRVNCRYCWGENHLYQWRDIAEFDKAAA
                     QASRDGKPEPEYGGLGFVDNAIPNPDCPKCCGEGTGQLYMADTTLLDGDARQLYAGSK
                     LGKYGVEILLEDKAAARRELLRLLSAGGALCADKRLQELEIERRRIENQKLRKEIETV
                     EDNEHPQPVAININVVDARLRSDEDDLSDA"
     gene            3255..4556
                     /locus_tag="IU325_10715"
     CDS             3255..4556
                     /locus_tag="IU325_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP5809495.1"
                     /translation="MISPTLNVPQARFLSMPHKFKAYIAGFGSGKTWVGCGGICKGIW
                     EHPGINQGYFAPTYPQIRDIFYPTVEEVAADWGLNVKINEGNKEVHFYYGRQYRGTTI
                     CRSMEKPQTIVGFKIGNALVDELDILPKEKARTAWRKIIARMRYKIDGLRNGIDVTTT
                     PEGFKFVYEQFVKAVREKTELASLYGLVQASTFDNEKNLPADYIPSLLESYPPELIKA
                     YLLGRFTNLTSGTVYHQFDRKLNNCEEVEQPGEPIYIGMDFNVGKMAGIVHVLRLGLP
                     CAVTEIINAYDTPDMIRLIKERFWPYDGHDYRKVREIYIYPDASGDSRKSSNASTTDI
                     AQLKQAGFNVVVNSSNPPVKDRVNSMNAMFCNANGERRYKVNVKRCPVYAESLEQQVW
                     DDKGEPDKKSGNDHPNDAGGYFIVKQFPIVKPTGRVTSLRI"
     gene            4559..5965
                     /locus_tag="IU325_10720"
     CDS             4559..5965
                     /locus_tag="IU325_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4055 domain-containing protein"
                     /protein_id="HAP5809496.1"
                     /translation="MADISTPNLDYNDMLEAWDINDALMGGTLEMRRQGENYLPKWPN
                     EDEDAYKKRLSVATLLPVYEESIKQNIGRIFAEPTVLSEETPEKIREYAENIDMEGSR
                     LDVWAQQFFSLAFQYGVAHALVDYPRTDMNEIRTKADENAAGGRPYVTMLNPRQVIGW
                     KSKVEKGKVVLTDLRIKEVIIIDGDDFGQKKVEQIRHIMPRRVEIYRRSEGTNGESVW
                     MLHESWNTSRDDIPLVTLYTKKTGFMRGTPPLLNLGLLNIKHWQSQSEQDNILHVARV
                     PLLVAYGLDRNEELTVGASTATIFEDRTKNGLEYVEHSGAAIESGETSLEKLENQMRH
                     AGAKLLRAENTSTKSVDQTNEERMQEHSPLYTMANSLEDALDNILQIMAEWSGESCGG
                     NVDVRTELDVSAQVFDSSSALAVQSLRQGGDIRQIDAVRVLQALKFIDQDSRPEEVID
                     ELKNQSVMLMEINDANRE"
     gene            5949..7061
                     /locus_tag="IU325_10725"
     CDS             5949..7061
                     /locus_tag="IU325_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001645719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809497.1"
                     /translation="MQTVNERLRDESIAHAVWISRYSTGVAARMVKILNDSDAELTAR
                     LLVALDSLDPGSFTVTRLESLLASVREVNRAAINSMFTSLSGELNELAIYEAGYQLSL
                     FDSLLPDFVADVHPLVGISSDALYAAAMARPFQGRLLSEWALDLEADRLRRITNTVRQ
                     GFLLGDTNEQIARKIRGHVSKGFQDGALQMSRANAASIAKTAVGHLAATARESFASAN
                     NDLIKGKQWLSTLDNRTTPQCRIRDRLKYTLDNKPVGHSVPYLHGPGKIHFCCRSTET
                     FILKSAKELGIDVRDISPAERASMDGVVAGDTTYREWFLRQPYTRQKQIVGETRAKLI
                     RDGGMSPDEFYTDKGEWLTLKQLRERDAQVFRKAGI"
     gene            7166..7930
                     /locus_tag="IU325_10730"
     CDS             7166..7930
                     /locus_tag="IU325_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016160818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809498.1"
                     /translation="MKLKTVEINGKQYAEIDTAGLPVYVHDDGKEIGFDAPLAIKKIT
                     ELNGEVKNHRLAKEAAEEKLAKFAAIEDPKKAIEALEMLSKIDQKKLIDAGQVDQVKA
                     EITKNFQQQLDEEKQRSQMLETQLYDSMIGGSFAGSKYIADKIAIPADLLQARFGQAF
                     KVEEGKIVAYDASGNKIYSRAKPGELAQFDEALEFLVENYPQKDYILKASGNNGGGSR
                     PTQHDIGQKTMKRSAFDALDVAGKQNALKDGITIVD"
     gene            8029..9168
                     /locus_tag="IU325_10735"
     CDS             8029..9168
                     /locus_tag="IU325_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 coat - protein 5 family protein"
                     /protein_id="HAP5809499.1"
                     /translation="MAGNTLTGLIPTIYTALDIVSREQVGFIPAVAKNAKADAAAKDQ
                     TVTAPVAPEAKTEDIVPGPSAPNTGDQNIGTVDVKITKSKMAPVKWNGEEQLALGPSG
                     TYNTILADQFKQAFRALANEVEADLGALYFGASRAVGTAGTTPFGVKDDLSDAALARQ
                     VLEDNGAPTTDLQMVLGSTAIANLRGKQSVLFKVNESGTEQLLREGVLGRLEGFNIHS
                     SAGVKRAPKVAATGYLVNGEKKEGDVLISIDTGSGSISAGQIVTFAGDPNQYVVAAAT
                     SNLITLAAPGLRQDLADDTAITVVGSFTANMAFDRNAFLLASRTPAMPEGGDNADDVM
                     NVTDPISGITFQIALYRQYRQVRYEVGLAWGVSSVKPAHGCLILG"
     gene            9391..9786
                     /locus_tag="IU325_10740"
     CDS             9391..9786
                     /locus_tag="IU325_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein singed"
                     /protein_id="HAP5809500.1"
                     /translation="MITYVTCDDVDNAFGNAWTSENAKNKAVLMANAWLNGFNLKINP
                     SRIPEEVKLAGAYAARIASFGKLFQQKNDSGVVISKVVSADGVSVSKSFAELSANSTV
                     LLEPDLQLAIALLKPYGLSRSQVRVVRGG"
     gene            9786..10169
                     /locus_tag="IU325_10745"
     CDS             9786..10169
                     /locus_tag="IU325_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP5809501.1"
                     /translation="MGLREEIQSEVAAAFDEDLADAVSDFSGSYVTHRHWDPVTETGG
                     ESTAIYTGRGVLTRYKLGRIDGINILHGDLKLTALVCEVTDKPAVDHIIEIYDPVLRQ
                     LQRYEVITASVDPSASVYSIQLRRA"
     gene            10170..10550
                     /locus_tag="IU325_10750"
     CDS             10170..10550
                     /locus_tag="IU325_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP5809502.1"
                     /translation="MAKAWDIEPSIFAGMIEEDVGLKIRYIAMQILTAIDIAAPVDTG
                     RFRNNNLVSLQHPDFGISDNVDPNGTIAVQRGNGVISKAANYGVIYIQNNLPYAEDLE
                     NGHSQQAPTGVYANAFHGVLQAYK"
     gene            10547..10939
                     /locus_tag="IU325_10755"
     CDS             10547..10939
                     /locus_tag="IU325_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004916408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HAP5809503.1"
                     /translation="MTFTEIRNTVISRMTAQTIIDGKDVLYPNAPTFDPSGKLIWARL
                     SNIPGQAGVNEIGAGPIVYRTGIIIIQLFVPAGSGSKLITETADKLRELFEFQDDDRL
                     SYQAVSSIAVGEKNGWFQLNLQIPYRAL"
     gene            10966..11928
                     /locus_tag="IU325_10760"
     CDS             10966..11928
                     /locus_tag="IU325_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809504.1"
                     /translation="MSSGAKVISAFIRETVAGTTPASGDWSLLKRTSWGVKPTQNKGE
                     NNEIGGSRMAQGATPGTVDVGGDVGTKFRWGQHDDFLASCFGAEWSGDSLTMGNERIT
                     FSLATYASDVGIASVVRGAQVGSWKMQIPNDGDITATVTFAGLDWESKADDTNFIKGE
                     PVDSAGKLRYSFKEVSAVSLNGVAGGNGFCIDSFDIQFDNKLQTQRCIGTGSPYAGAN
                     IPTTFTPSGTVTLSWSKAAWEIWSKTLTGETVPFSFTLSNGEGAYTFSFPKVQVSGEW
                     PDGGNSDIIQVQLSITAADEAPTITRKKNSPAAVIAKASAEAIS"
     gene            12079..12438
                     /locus_tag="IU325_10765"
     CDS             12079..12438
                     /locus_tag="IU325_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809505.1"
                     /translation="MVASSDNDQYRSRNALIRRHIEKMDASLHVGTKEFDISKVSEVD
                     SVDDLLIDNAARYLLKDWKGVGELVNGVEVALEYTAERGIALLKQNPELYWQILAEAA
                     SIAQGKEQQKQDTIKKP"
     gene            12912..16145
                     /locus_tag="IU325_10770"
     CDS             12912..16145
                     /locus_tag="IU325_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP5809506.1"
                     /translation="MAEQTSRLAIIIDSTGAKNNADNLTSSLVKMTQAGETAANSAGK
                     VTKATEDEKNALAKLKAAIDPVGAAIDTVGRRYSELKKFFDKGLIDKEEYEFLVRKLN
                     ETTEELSGVAQAQREAEKAGKLAAAQQEAQAQAFQRMLDKIDPLAAALRNLEQQQDEL
                     NAAFASGKINGSQFENYSRKIQETRRELTGEAQAEREAAKAHDEQVAALQRLIAQLDP
                     VGTAFNRLVEQQKQLNEAKAKGMLSPEMYEELSGKLRAMRSELEVTQSQLSKTGMSAK
                     QTAFAMRMLPAQMTDIVVGLSTGQSPFMVLMQQGGQLKDMFGGIGPAIKGVGSYVLGL
                     INPFTLAAAAVGVLGLAYYKGSQEQDEFNKSLILTGNQLGTTSGQLADIVQRAGNAAD
                     STTGAAAAVLNQLVRSGKVASSSLEQVTTAIVKTSEVTGISTEQLVNDFNEIAKDPVS
                     AISKLNDQYHFLTIATYNQIKALQDEGNQQEAARIATEAYSSSMIQRTNQIKENLGYL
                     ETAWKAVADSAKWAWDSMLDIGREASLDQKISDVLRQIDEIEKNTRPGVFGLGGIGDG
                     GAQNKRLARLKQQLGVLQAEKIAQDVLNSSINDYNKRQQEGIELRQRADAFSKQYQTR
                     EQQRASELAKLEKLKNQYSKEEYNNLIAQINERYKDPKQPKAKGYSDDAAQRMIDHLN
                     QQNALLSSQAELTVKLSSSEQELVKWRQRIADLESRPSSKLTQDQKSLLLHREEITAL
                     MEKNVAIEKNNRLIKESAEITAWRDSLQASIDNRQQGYDIQIAGYGVGDKNQQRQQEL
                     LRIEHGYNNQRLQLERDYADKSRGMSDHVFQEKMQALNDALEREKEIVRQKNEQLDIQ
                     AGDWVSGASQGFNNWLDDTKDISEQIKSTTTQMFDGMTDALGDFVTTGKANFRSFATS
                     VISDLSRIALKASITGIFDSISNSSSGGILGTIGSAISKFIPNAKGGVYESPSLSTYS
                     NGIYDSPQFFAFAKGAGVFGEAGPEAIMPLTRTSDGSLGVRAINSKSGNGGGDITYAP
                     VYQITIQNDGQNGEIGPQAIKALMGMVDQRVQGNLLNMRRDGGMLSG"
     gene            16138..16476
                     /locus_tag="IU325_10775"
     CDS             16138..16476
                     /locus_tag="IU325_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP5809507.1"
                     /translation="MANTEEFHWLPEDGMKTENKPSIKTVRFGDGYEQRSPNGLNHSL
                     RVFTCDFKVEANERDSFEKFLARHEGYKSFFWRPPGINRKIRVVCRTWSATEHITYTD
                     FSCQFDEVVI"
     gene            16476..17174
                     /locus_tag="IU325_10780"
     CDS             16476..17174
                     /locus_tag="IU325_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HAP5809508.1"
                     /translation="MQDIQQETLNECIRAEQSASVVLWEIDLTEVGGDRYFFCNEQNE
                     KGESVTWQSRQYQAYPIQGSGFEMNGKGASARPTLKVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPVVADEYDQPTSEITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            17180..17923
                     /locus_tag="IU325_10785"
     CDS             17180..17923
                     /locus_tag="IU325_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP5809509.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRAPEGERYFPCVNISGEPE
                     VYFRMAPEDWLQAEMQGEIVALVHSHPGGLPWLSEVDRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTMFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNMEAS
                     GFYRVPLSSAQAGDILLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLAAVSTFV"
     gene            17860..18468
                     /locus_tag="IU325_10790"
     CDS             17860..18468
                     /locus_tag="IU325_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HAP5809510.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLND
                     GWYQVRIAGRDAGETELSARLNESLANGAVIHIVPRLAGAKSGGVFQAVLGAALIAVA
                     WWNPVGWLGAAAVSGMYAAGASMILGGVAQMLAPKARTPTAASTDNGKQNTYFSSLDN
                     MVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            18529..22008
                     /locus_tag="IU325_10795"
     CDS             18529..22008
                     /locus_tag="IU325_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HAP5809511.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGRYTLTVRAVNAWGQQGDPASVSFRIAAPSAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIYMNEVFLKYLTAPTITSGGNP
                     PAFSLTPDGRLTAKNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVG
                     KAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKHEQDHTDIYSSCRLI
                     VRKNGAEIYNRTALDNTLIYTGVIDMPAGSGVMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            22076..22675
                     /locus_tag="IU325_10800"
     CDS             22076..22675
                     /locus_tag="IU325_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HAP5809512.1"
                     /translation="MRKLCAVILSAVVWLVAAGMPASAAEHQSTLSAGYLQTHTDMPG
                     SDDLKGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGMAYSRVSTFSGDYIRVTDNKGKTHDVLTGSDDGRHSDTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            22740..25142
                     /locus_tag="IU325_10805"
     CDS             22740..25142
                     /locus_tag="IU325_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP5809513.1"
                     /translation="MAAVKISGVLKDGAGKPIQNCTIQLKAKRNSTTVLVNTVASENP
                     DEAGRYSMDVEYGQYSVTLLVEGFPPSHAGTITVYEGSRPGTLNDFLGAMTEDDVMPE
                     ALRRFEEMVEEAARNAEAASQSAAAAKKSETAAASSKNAAKTSETNAANSAQAAAASQ
                     TASANSATAAKKSETNAKNSETAAKTSETNAKSSQTAAKTSETNAKASETAAKNSQAA
                     AAESESAAAGSATSAAGSATAAANSQKAAKTSETNAKSSQTAAKTSETNAKASETAAK
                     NSQAAAAESESAAAGSASAAAASATASANSQKAAKTSETNAKVSETAAANSAKASAAS
                     QTAAKASEDAAREYASQAAEPYKYVLQPLPDVWIPFNDSLDMITGFAPGYKSITVGDD
                     VIALPSEKVVSFTRASTATYIDKSGCFAESAINEPRFEKDGLLIEGQRTNTFSYTNTP
                     VSWNYDTANLTITTGVDEYGFSYGLFGVKETSTTERATLISTGYTRVISVSANESVTL
                     SCRVKKVSGDGIITLRPRISYVNDDGSSNTLTAGAYIDCETGDMLSYSGGEAATYNIF
                     RESNGWIRVEFTYKSPEAKNMYGRFEFGAHQRSIKSGDKLMLTTPQFEKGLNASSFII
                     TTEVGATRASDQVIIPIPFNWATPPVSVLMEVNVNWDSEMPNLEGSARLLNISITGAT
                     TEVSDESYMYFGFTTRGKRLIITNGKGTKTEYKAYGNREKRKFVTGFKFTEDKQLQVV
                     VDGILGSSSPSLHTLQRYTAGNINIGGQSSSGNRHLFGHVKNLRIWHKELTEAQMGES
                     IK"
     gene            25139..25420
                     /locus_tag="IU325_10810"
     CDS             25139..25420
                     /locus_tag="IU325_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809514.1"
                     /translation="MKDLTLKFADRADFSAFMESIGYYDDESMQDDILIDVIGNVYKE
                     TGELTEDGEPVCVKEDGYFVNVRIINDAKKSSIFDEYVVAVEHQLRGWM"
     gene            25430..26119
                     /locus_tag="IU325_10815"
     CDS             25430..26119
                     /locus_tag="IU325_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HAP5809515.1"
                     /translation="MATSTVIPEDIKTLKGDVSKTKEDISSINGKVSTLQIDMTSAKQ
                     DISTRYTKTEVDNKLKNKVEANDLESGRYGGDFYPLTGREAFYLWNLATTTAAANLYL
                     NPDPAISSVLRSTSSIRYKHSVETIDSEHADLIFKMRPVWYRSQCENDRRDWGFYGLI
                     AEEVGEIAPQFVHWRPANENDAPEAISSNGLVAEGVMYERLVVPLIDHIQKLTERVEE
                     LESELKLLSTS"
     gene            26145..26438
                     /locus_tag="IU325_10820"
     CDS             26145..26438
                     /locus_tag="IU325_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809516.1"
                     /translation="MDITPFLHALCAVAAQVLVGLFTGNWAYGAIAGCTFFIAREHTQ
                     AEYRWIEMFGHGKRMNMPWWGGFDPRAWDVASLMDFAVPVVACLLVWLLVNRG"
     gene            26458..26577
                     /locus_tag="IU325_10825"
                     /pseudo
     CDS             26458..26577
                     /locus_tag="IU325_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518755.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26651..27017)
                     /locus_tag="IU325_10830"
                     /pseudo
     CDS             complement(26651..27017)
                     /locus_tag="IU325_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586336.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
BASE COUNT         7264 a         5792 c         7438 g         6523 t
ORIGIN      
        1 ggttttgggg ctactgcatc gtcattcgga gtgcttaatg acattgcaaa atatttaatg
       61 tgcatagcta tgattcttgg tcgccttgaa atttatcctg ttattatatt gttttcaggt
      121 tttttttggc gctcctaata tatggctgat ttataattgt gagtttaata ttatattgac
      181 tcactcattg atccaatacc taactttacc agcaacacct ccgcccccag tagcactggc
      241 tgctggggtg cgttttattc ataaagcaag gctgtatgag cgagaaatta aagatagtct
      301 atcgcccatt acaagaattg tcaccgtatg cgcacaacgc caggacgcac agtactgagc
      361 aggtggcaca actggtagaa agtattaagc aattcggctg gactaatccg gtgctgattg
      421 acgaaaaggg cgaaattatt gcgggtcacg gtcgtgttat ggcggctgaa atgctcaaaa
      481 tggattctgt tccggtcatt gttctgtctg gcctgacgga tgagcagaaa aaggcgtacc
      541 gcctggcaga taatcgccta ccgatgaatg ctggctggga tgaagatcta ttgcggatgg
      601 agctgtcgga cctaatcaat gctgattttg atgtctccct gacaggcttc ggcccgacag
      661 aaattgatga actgttgacg gatgttttgc ccggtacagg aaataaggag gagccgtata
      721 cgacgaaaat tgatacgcct gtttatgagc cgtcgggcgg taaaccggat atcagtgaac
      781 tgtacgacga tacgaaaact caggagctga tcagccggat acgttcggcg tcccttgatc
      841 ctgatattga gaaattcctc ctgtgtgcgg cagaacgtca cacggtgttt aatttcagca
      901 gaattgcgga ctattacgct catgcccccg ctgaaattca gtgccttttc gaggagtcgg
      961 cgctggtgat cattgattat cagcaggcta ttgaaaatgg atttgtccga atgacgcagc
     1021 gcatggtggc gatcatgcat agcggggagg aggaatatgc gtgatgattt ttgcgccttt
     1081 attctgactc acgggcgacc ggacaaagtt ctgacttacc ggacgttgcg tcgtgctggc
     1141 tataccggga aaatttttat cgttgttgat gatgaagata agacacggca tcagtacatg
     1201 gctgaatttg gtgaacaggt gctggtattt tccaaagccg atattgccag tcgttttgac
     1261 gaagccgata attttggtga ccgccgctca attttttacg cccgtaatgc ctgtttcgac
     1321 ctggcaaaac tggtcgggtg taaatacttc attcagctcg atgatgatta tcacgagttc
     1381 cagtttcggg tggatcgcaa ctatgaccag gcctatttcc cgataaggaa actggatgcg
     1441 atcctttctg aaatgctggc gtattacgaa tcaatacccg cgctttccat cgctatgtcg
     1501 cagggcgggg attttcttgg tgacaatggc ggccatgctt cgtgggtgaa acgcaaggca
     1561 atgaacagct ttatctgttc ggttgatcga ccgttctcat tcatggggcg cattaacgag
     1621 gatgtgaata cgtacacgaa tctcggtcgc tgtggtgaat tgtttatgac gatcggtgct
     1681 gtccagttag ggcagaaaca gacgcagaaa aacagcggcg gaatgaccga gctgtatctg
     1741 gattccggaa cctacgttaa aagtttttac tccgtcatgt atgcgccgtc gtgcgtaaaa
     1801 atctcactga tgggtgccag ccataaacgc attcaccatc aggtcacctg gaacaacgct
     1861 gcagtaaaaa tccttcacga aaaatacagg aagaagacac cctgcatatc aatgggggtg
     1921 acaaatgatt ccgtattcga aagtcgagtc tctggcagcg tgccggatga ctgcacaaca
     1981 aatcgctgac gttctggatg ttgatctgaa ccgactgaaa gaaaatcggg aagcaatgac
     2041 agatttttat gcggccatcc gtaagggcag agcgaaaggt gaagccgagt tacgagcggc
     2101 attgtttaag cttgccagaa aaggggatgc ctttgctctg cgcgaactac tcagggtgga
     2161 taaaaatcag gactaactga tgagcagacc ggactggggg gcgttgcagc aggagtatat
     2221 tgctgaatac acccgctccg gtatatctcc ggtggcatgg tgtgaagcaa ggggactgaa
     2281 ctacgcaaca gcccgtcgtt acatcaaaaa aactccgaaa aatgcgcaga aagaaatgcg
     2341 caaaactgcg caaaaaagtg cgcgaaaaaa aactgcgcag actgcgcaaa ggcagaacgg
     2401 aaaatctcag aaaaaaaagg ctgtatccga tgcactcctg attgaggggg acacggaaga
     2461 aatttcgttc tgccccgatg aattcggtat ttctgaccag caggcaaaat tcgccatgtt
     2521 ggttgctcag gggaaaaagc cgacagaggc ataccgactg gctggttatg aggggcaagg
     2581 tgcgacagct aacagcaacg ccagccgtat gcttagaaat gccagggttt atcgtgctat
     2641 cagctacttc cgcaatcagt accagaaacg ccataccgca gacctggatt tactggtgag
     2701 ccagttgatg gccattgtcc aggccgaccc caatcagttg gcacaatttc gccgtgttaa
     2761 ctgccgttat tgctggggcg agaatcatct ctaccagtgg cgtgatattg cagaattcga
     2821 taaggctgcg gcacaggcct ccagagatgg taaacccgag ccggaatatg gaggcctcgg
     2881 ctttgttgat aacgccatac ccaatccgga ttgcccgaag tgctgcggtg agggaacggg
     2941 acagctttat atggctgata ccacactgct tgatggggat gcgcggcaat tatatgcagg
     3001 gtcaaagctc ggaaaatacg gtgttgagat cctgctggag gataaggctg ccgcccggcg
     3061 tgaattgttg cgtttgcttt ctgctggcgg ggcattatgt gcagataagc ggctacagga
     3121 actggaaatt gaacggcgca gaatagaaaa ccagaagctg cgcaaagaga tcgaaacggt
     3181 ggaggataat gaacatcccc agcctgtggc gatcaatatt aatgtggttg atgccagatt
     3241 aaggagtgat gaagatgatc tctccgacgc ttaatgtgcc tcaggcgcga tttctttcaa
     3301 tgccccataa atttaaagcc tatattgctg gttttggctc gggcaaaaca tgggttgggt
     3361 gtggcggcat atgcaagggg atttgggagc atccaggtat aaatcaggga tattttgcgc
     3421 caacgtatcc ccaaattcgc gatatttttt accctacagt ggaagaagtt gctgctgact
     3481 ggggattgaa cgtaaaaatt aatgagggaa ataaagaggt tcacttttat tacggacgcc
     3541 agtatcgggg aaccactatc tgcagatcga tggagaagcc acaaacgatc gtcggtttca
     3601 aaattggtaa tgcgctggtg gatgaactgg atattttgcc gaaggaaaaa gccagaacgg
     3661 cgtggcgcaa gataattgcg cgtatgcgtt ataagattga tggacttcgc aacggtattg
     3721 acgttacaac cacgccggaa ggattcaaat ttgtctacga gcagtttgtt aaagccgtgc
     3781 gtgaaaaaac agagctggcc tcactgtatg gtctggtgca ggcatctact ttcgataatg
     3841 aaaagaacct gccagcagat tacattcctt cacttcttga atcataccct ccagagttga
     3901 ttaaagccta tcttctagga cggtttacta acctgacaag tggtactgtt taccatcagt
     3961 ttgaccggaa actgaataat tgcgaagaag tggagcagcc aggggagccg atttatattg
     4021 ggatggattt taacgttgga aagatggcgg gaatcgtcca tgtgctgcgt ttggggcttc
     4081 catgtgcggt aactgaaatc atcaatgcct acgatacgcc ggatatgatc cgcctcatta
     4141 aagaacgctt ctggccgtat gacgggcatg attaccggaa agtgagggag atttatattt
     4201 atccagatgc ttccggagat tccagaaaat caagtaacgc aagtacgacg gatatagccc
     4261 agcttaagca ggcaggtttt aacgttgtgg tgaacagctc gaacccgcca gtaaaagatc
     4321 gcgttaactc aatgaatgca atgttctgca atgccaatgg tgagcgtcgc tataaagtta
     4381 atgtgaagcg ttgtccggta tatgccgaat ctctggagca acaggtctgg gatgataagg
     4441 gggagcctga taaaaaatct ggcaatgatc acccgaatga tgccggaggt tatttcatcg
     4501 ttaagcaatt ccctattgtc aaaccgaccg gaagagtcac atcacttcgg atttaattat
     4561 ggctgatata tcaacaccca acctcgacta taacgatatg ctggaggcgt gggatattaa
     4621 cgacgcattg atgggcggta cgcttgaaat gcgcaggcag ggggaaaatt atctccccaa
     4681 atggcctaat gaagatgaag acgcttataa aaaacgcctg tctgtggcta cgctacttcc
     4741 tgtgtatgaa gaaagcatca aacaaaatat tgggcgcata tttgcagagc cgacagtatt
     4801 gagtgaggaa acgccggaaa aaatcaggga atatgcagaa aatatcgaca tggaggggag
     4861 ccgactggat gtgtgggcgc agcaattttt cagtctcgca tttcagtatg gtgtggcaca
     4921 tgcgctggtg gactatccac gaacggatat gaacgagatt cggacaaaag ccgatgaaaa
     4981 cgcggccggt ggtcgcccgt acgttacgat gctgaatcca cgccaggtta ttggatggaa
     5041 atcgaaagtt gaaaaaggga aagttgttct cactgatttg cgtataaaag aggtcatcat
     5101 tattgatggt gatgatttcg ggcagaagaa ggtggagcag atccgccata ttatgccccg
     5161 tcgagtagaa atttatcgac gcagcgaagg tactaatggc gaatctgtct ggatgcttca
     5221 tgagtcatgg aataccagcc gtgatgatat tcctctggta acactgtata cgaagaaaac
     5281 agggtttatg cgtggtacac cgccattgct taatcttggc ttgctgaata tcaagcactg
     5341 gcaaagccag agcgagcagg acaatattct tcatgttgcc cgcgttccat tgctggtggc
     5401 ctacgggctg gacaggaatg aagaactgac ggttggtgca tccaccgcta cgatttttga
     5461 ggacagaaca aaaaatggcc tcgaatatgt tgaacatagt ggcgcagcga tagaatctgg
     5521 cgaaacatca cttgagaagc tggaaaatca gatgcgtcat gccggcgcta aacttctgcg
     5581 ggctgaaaat acatccacca aatctgttga tcagactaat gaagagcgga tgcaggaaca
     5641 ctcgccgctt tatacaatgg cgaactccct cgaagatgcc ctcgataata ttctccagat
     5701 aatggcggaa tggagcggag agagttgcgg tggcaatgtg gatgtgcgca ctgaactgga
     5761 tgtatctgcc caggtgtttg actcatcctc cgcgctggct gttcagtcat tgcgtcaggg
     5821 cggtgatata cgtcagattg atgcggttcg ggtgttgcag gcgttgaaat ttatcgatca
     5881 ggattcccgt ccagaggaag tgatcgatga attaaaaaat cagagtgtaa tgctgatgga
     5941 aatcaatgat gcaaaccgtg aatgaacggc tgcgtgatga atcaattgct catgcagtct
     6001 ggatatcccg ctatagcact ggtgtggctg ccagaatggt gaaaatactg aatgacagcg
     6061 atgcggagct tacagctcgc ctgctggtag cactggacag cctcgatcct ggtagtttta
     6121 ccgttacgcg cctggagtca cttctggcga gtgtcagaga ggttaaccgc gctgctatta
     6181 acagcatgtt taccagtctc tccggagagc tgaacgagct ggcaatttat gaggctggtt
     6241 atcagttaag tctgtttgat tctctgctac ctgattttgt tgctgatgtt caccctctgg
     6301 ttggtatatc ttctgatgca ctttacgccg ctgcaatggc gcgaccattc cagggacgac
     6361 tgctcagtga gtgggccttg gatcttgagg cggatcggct cagacgcata acaaatacgg
     6421 tgcgtcaggg ttttttgctg ggggatacca atgagcagat cgcaagaaaa attcggggac
     6481 atgtcagcaa gggatttcag gatggtgcat tgcagatgag ccgggctaat gcggccagca
     6541 ttgcaaaaac agcggttgga catcttgctg ctactgcccg tgagagtttt gccagcgcga
     6601 ataatgattt gattaagggt aagcaatggt tatcaacgct tgataatcgt actacgccac
     6661 aatgtcgaat cagggatcgc ctcaaatata cgctggataa taaacctgta ggtcacagtg
     6721 tgccttattt gcatgggccg ggaaaaattc atttctgctg ccgctcaacg gaaacgttca
     6781 ttctgaaatc agcgaaggaa ctgggtattg atgttcgtga tatttccccg gctgagcggg
     6841 ccagcatgga tggcgtggtg gccggagata caacctacag ggaatggttt ttgcgtcagc
     6901 cttacaccag acaaaaacag attgtggggg aaacccgggc aaagctgatt cgggatggcg
     6961 gtatgtcgcc agatgaattt tacaccgata aaggcgaatg gctgacgctg aagcaactcc
     7021 gtgagcgtga tgcacaggta ttcagaaaag cagggattta aataaatcat ttattacaac
     7081 aggctacctt cgggtggcct tttttattgc tgcgatccgg atggtgagca gcgtaactgt
     7141 cggaagactt aaaccaggta ctaatatgaa actgaaaaca gtcgagatta acggaaaaca
     7201 atacgcagaa attgatactg ctggcctgcc agtttatgtg cacgacgatg gtaaagaaat
     7261 cggcttcgat gcaccgctgg cgataaaaaa aattacagag cttaatggcg aggtaaaaaa
     7321 tcatcgcctg gctaaagaag ctgcagagga aaaactggct aagtttgccg ctatcgaaga
     7381 cccgaagaag gcgatcgagg cactggaaat gctgtcaaaa atcgaccaga aaaagctgat
     7441 cgatgcggga caggttgacc aggttaaggc agaaattacg aaaaattttc agcagcaatt
     7501 agatgaagaa aagcaacgct ctcagatgct ggagacgcag ctttacgatt ctatgattgg
     7561 cggtagtttt gcgggttcaa aatatattgc cgataaaatt gcgatcccgg cagatttatt
     7621 acaagcccgc ttcgggcagg cattcaaagt ggaagaaggg aagatcgttg cttatgacgc
     7681 ttccggcaac aaaatttatt cccgcgcgaa gcctggcgaa ctggcgcagt ttgatgaggc
     7741 gctggagttc ctcgtcgaaa attaccctca gaaagactac atcctgaaag ccagtggcaa
     7801 caatggcggc ggctcccgtc cgacacagca tgatattggt cagaaaacga tgaaacgctc
     7861 tgcttttgat gcactggatg ttgcaggtaa gcaaaacgca ttgaaagacg gtatcacaat
     7921 tgttgattaa cacatttgtc agcttccgga tgggagctgg tgtcagggct ggatagctca
     7981 ttactccatc tattcacaat tacgcaaatt tttaaggaat atttaattat ggctggaaat
     8041 accctgaccg ggttgatccc gactatttat accgccctgg atattgtatc ccgtgagcag
     8101 gtaggtttta tccctgcggt agcaaaaaac gcaaaagctg acgccgcagc aaaagatcag
     8161 acggtaaccg cgccagttgc gcctgaggcg aaaaccgaag atatcgtacc ggggccgtca
     8221 gctccgaata ccggtgatca aaatattggc actgttgatg tgaaaattac taaatccaaa
     8281 atggcaccgg ttaaatggaa tggtgaagaa caactggctc ttggcccttc agggacttac
     8341 aacaccattc tggctgatca gttcaagcag gcttttcgcg cccttgcaaa cgaggttgag
     8401 gctgatcttg gtgcgttgta tttcggtgct tcccgcgccg tgggaaccgc agggacaacg
     8461 ccgtttggtg ttaaagatga tctttctgat gctgctctgg ctcgtcaggt tctggaggat
     8521 aacggcgcac cgacaaccga tctgcagatg gtgcttggct ccactgccat tgctaatttg
     8581 cgcggaaaac agtctgtact gtttaaagtg aatgaatccg gcactgaaca gctactgcgt
     8641 gagggcgtgt tggggcgtct ggagggattc aatattcaca gttcggcagg tgtaaaacga
     8701 gcgccaaagg ttgctgcaac tggttatctc gtgaatggcg agaaaaaaga aggcgatgtt
     8761 cttatttcca ttgatactgg ttcggggagt atttctgcag gtcagattgt tacgttcgct
     8821 ggcgatccga atcagtatgt tgtggcagca gcgaccagca atctgattac tcttgcagcg
     8881 cccggactgc gtcaggatct ggccgatgat actgcaatca cagttgttgg ctcctttact
     8941 gcaaatatgg cgtttgatcg caacgcgttt ctgctggcat cccgtactcc ggcaatgccg
     9001 gaaggtggcg ataatgctga tgatgtaatg aatgttacgg acccgatttc agggattacg
     9061 ttccagattg cactgtatcg tcagtatcgc caggtgcgct atgaagttgg actggcatgg
     9121 ggtgtttcat cagtgaagcc ggcacatggt tgtctgattc ttggttaaac attcaaacgg
     9181 ggcttcggcc ccatttttaa tggagggcat atggccggat taactaaaga gcaaagagca
     9241 cagcgtgaag ccgcacaaaa aacgcaaatt gagttagtgg ttatggtgac cgattatcag
     9301 atgtttcccg gcgcaccaac tatcgctaat gttcatcctg atgaagttga caactggaag
     9361 gctatgggct ggaaaactca ggagtaacac atgatcactt acgtgacctg tgatgacgtt
     9421 gataatgcgt ttgggaacgc ctggacgagt gagaacgcta aaaataaagc tgttttaatg
     9481 gctaatgcct ggcttaatgg cttcaacctg aaaattaacc catcccgtat tccggaagag
     9541 gtaaaacttg cgggagcata tgcagccaga attgcctcat tcggtaagtt gtttcagcag
     9601 aaaaatgatt ctggcgttgt tatcagtaaa gtcgtgtcgg ctgacggggt cagtgtatcg
     9661 aaatcatttg ctgagttgtc agcaaacagc actgtattgc ttgaacccga tttacagctt
     9721 gcgatagcac tgctgaaacc gtacggactt agtcgctcac aagtcagggt tgtgaggggg
     9781 ggataatggg gcttcgtgaa gagattcagt cagaggttgc cgctgctttt gatgaggatt
     9841 tagcggacgc cgtgagtgat ttttctggtt cttacgttac gcaccggcac tgggatcctg
     9901 tgacggaaac tggcggcgaa tccaccgcaa tctataccgg gcgaggcgtg ttgacacgtt
     9961 ataagctggg cagaattgat gggattaata ttttgcatgg tgaccttaaa ttaaccgctc
    10021 tggtatgcga ggttacggat aaacccgctg tcgaccatat tattgagatt tatgatcctg
    10081 tattaaggca gttacaacga tacgaggtaa ttacagcgag tgtagatcct tccgcatcag
    10141 tttactctat ccagttaagg agagcgtaaa tggcaaaggc atgggatata gaaccgtcaa
    10201 tatttgccgg gatgattgag gaagatgtgg ggctgaaaat tcgctacatc gctatgcaga
    10261 ttcttactgc tattgatatt gctgctccgg ttgataccgg acgtttcaga aataacaacc
    10321 tggtgtcgtt acagcatccc gattttggta tatctgataa cgtggatccg aacggtacga
    10381 ttgcggttca gcgtgggaac ggggttattt cgaaagctgc aaattacgga gttatttata
    10441 tccagaataa ccttccttat gcagaggatc ttgaaaacgg tcattcacaa caagcgccaa
    10501 ctggcgtgta tgccaacgct tttcatggtg ttttacaggc ttacaaatga cgtttactga
    10561 aatcagaaat acggttattt ccagaatgac ggcacagacg attattgatg gaaaagacgt
    10621 attgtatccg aacgcgccaa cgttcgatcc ttccggtaag ttaatctggg cgcggttaag
    10681 taatattccc gggcaggctg gagttaatga aattggcgcg gggccgattg tttatcgcac
    10741 ggggataatc attattcagt tatttgtccc cgcaggttct ggttcaaaac tcattactga
    10801 gacagccgat aaattgcggg aactgtttga gtttcaggat gacgatcgtc tcagttacca
    10861 ggctgtttcc tcaatagccg ttggcgaaaa gaatggctgg ttccagctta atcttcaaat
    10921 tccatatcgc gcgctctagc gcaattaatg acataggagg ctcctgtgag ttcaggtgca
    10981 aaggttatct cggcatttat ccgggagacg gttgcaggca ctacgccagc aagtggtgac
    11041 tggagtttat taaaacgcac aagctgggga gtaaaaccca cccagaataa aggcgaaaat
    11101 aatgagattg gtggctcccg gatggctcag ggggcgacgc ctggcactgt ggatgtcggc
    11161 ggtgatgttg gtaccaaatt tcgctggggt caacatgatg attttcttgc atcctgtttc
    11221 ggcgcggaat ggtcaggcga ttctctgaca atggggaatg agcgaataac attttctctg
    11281 gcgacctatg cgtccgatgt cggaattgcc tctgtcgtca gaggagcgca ggttggctca
    11341 tggaaaatgc agatccctaa cgacggcgat attacagcga ccgtaacctt tgccggactg
    11401 gactgggaat caaaggccga tgatacgaat tttatcaaag gcgaacctgt ggatagtgca
    11461 ggaaagctac gttattcgtt taaggaggtt tcagcagtaa gcctgaatgg tgttgccgga
    11521 ggtaacggtt tttgtatcga cagttttgat attcagttcg ataacaaact ccagacacag
    11581 cgttgtatcg ggactggctc gccttatgca ggagcaaata ttccgactac ttttacaccg
    11641 tccggtacgg tgacgctttc atggtctaaa gccgcgtggg aaatctggag taaaacactg
    11701 actggagaaa cagttccgtt cagcttcacg ctttcgaatg gagagggggc atacactttc
    11761 agtttcccga aggttcaggt gtcaggtgaa tggcctgatg ggggtaatag cgacattatc
    11821 caggttcaat tgagcattac cgcagcagat gaagcaccta cgataaccag aaaaaaaaat
    11881 tccccggctg ccgtgatcgc aaaagccagt gctgaggcga ttagttgatt ttccgttatt
    11941 cctcctgtgg tactgcccta cagggggacg cattgaatga ggttatggat gtttattctt
    12001 aatcagaaaa ttatcattgg tggagaacgc tggtttacgc caatgaagga cttaaaacct
    12061 gtagacgggt taaaactgtt ggtggcaagc agcgataacg atcagtatcg ctcccgtaat
    12121 gcattaatcc gtcgccacat tgagaaaatg gatgccagtt tgcacgtcgg aacgaaggag
    12181 tttgatattt caaaggtttc cgaggtggat tctgttgatg atttactcat tgataatgcc
    12241 gctcgttatc tgctgaaaga ctggaaaggg gttggtgaac tggttaatgg tgttgaggtt
    12301 gcactggaat atacggcaga acgagggatc gcgctgctta agcagaatcc agagttgtac
    12361 tggcagatcc ttgcagaagc agccagcatc gcccagggta aagagcagca gaagcaggat
    12421 acgataaaaa agccatagct gcccagcggt ggttatcgga gttcggggga gaaagggggg
    12481 aaaaggcaag atggaagcga gaaaaactca ggttgccacc gataccggaa ccagaaatag
    12541 acccggtgct taaggagttg ttgtacgcct attcggtaat atcccgtgcc cgacgttatg
    12601 ctggaatggc tggggtgcct ttgcctttat ctctgacaga gataaatgaa tatttagcca
    12661 ctcatccggt attgattgag cgcgatgaat ttgaagcagt gatctttgca ctggatgacc
    12721 agtattttca ggagcagtgt gtgtagttgt taattacgta cactctgtta cagagatgtg
    12781 atggtgtctt taattaaatc gatgatgctc ctggagaaaa gcattgcgtg gcctcgtaat
    12841 cgctatatct actattatgt cgcctgaaac ccacttcgcg gtgggttttt tgttgtcagg
    12901 agttttaata aatggcagag caaacctcgc gtctcgcaat aattattgat agcactggag
    12961 cgaaaaataa tgctgacaat ctgacctcct cattagtcaa aatgacgcag gctggggaaa
    13021 ctgctgcaaa tagcgcaggg aaagtgacta aggcaacaga agatgagaag aacgcgctcg
    13081 caaaattaaa agcagctatt gatccagttg gtgccgcaat tgatactgtc ggtcgacgct
    13141 attctgaatt aaagaaattt ttcgataaag ggcttattga taaagaagaa tatgaatttc
    13201 ttgtccgtaa acttaatgaa accacagagg aattgagcgg ggttgcgcaa gcgcagagag
    13261 aagccgagaa ggccggaaaa cttgctgccg cccagcagga agcgcaggct caggcctttc
    13321 aaagaatgct ggacaagatc gaccctctgg ctgcggcgct aagaaatctt gaacaacagc
    13381 aggatgaact taatgctgcg tttgcatccg ggaaaataaa tggttctcag tttgagaatt
    13441 atagccgaaa aatacaggaa acacggcgag agcttaccgg agaggctcag gcagagcgag
    13501 aagcagcaaa agcgcatgat gaacaggttg ctgctttgca acgtctgatt gctcaacttg
    13561 accctgtcgg aactgctttt aatcgtctgg tagaacaaca gaaacagctc aatgaagcaa
    13621 aagctaaggg gatgctctct cctgaaatgt atgaggaact ttctggaaaa cttcgtgcta
    13681 tgcggagtga gcttgaggtt actcaatctc aattaagcaa aaccggaatg tcggcaaaac
    13741 aaacggcttt tgctatgcgc atgttgcctg cacaaatgac ggatattgtt gttgggttgt
    13801 ccactggtca gtcgcctttt atggtgttaa tgcagcaggg cggccagcta aaagatatgt
    13861 tcggtgggat aggcccggcg atcaaaggtg ttggttctta tgtgctggga ttaattaatc
    13921 cttttaccct ggccgcagca gccgttggcg tcttagggct ggcttactat aaaggctctc
    13981 aggagcagga cgaatttaat aaatctctta ttcttaccgg aaatcagttg gggacaacca
    14041 gcgggcaatt ggccgatata gttcaacgtg ccgggaatgc ggctgattcg acaactggcg
    14101 ctgcggcggc agtattaaac cagcttgtgc gttcgggaaa ggtagcgagc agttcgctgg
    14161 agcaagtgac gacagcgata gtaaaaacga gcgaagtaac aggaatatca accgaacaac
    14221 tggttaatga cttcaatgaa attgcaaagg atcctgtcag tgccatatca aaacttaatg
    14281 atcagtacca ttttctgaca attgcgactt ataaccagat taaggcgcta caggatgaag
    14341 ggaaccagca ggaggccgcc cgcattgcga cagaagcata ctcatcctca atgatccagc
    14401 gcaccaacca gattaaagaa aatcttggtt atcttgagac tgcatggaaa gctgtcgcag
    14461 actccgcaaa atgggcatgg gattccatgc tggatattgg ccgtgaggcc tcccttgatc
    14521 aaaaaatctc agatgttctc cgtcaaattg atgaaataga aaaaaatacc cgacccggag
    14581 ttttcgggtt aggtggcatt ggagatggcg gagctcaaaa taaaaggctg gcacgattaa
    14641 agcagcaatt gggcgtactt caagcagaaa aaattgctca ggacgtacta aattcatcaa
    14701 taaacgatta caacaagcga caacaggaag gaattgaact cagacagaga gcagatgcct
    14761 tttcaaaaca atatcagacc cgggagcagc agagagctag tgaacttgca aaactggaaa
    14821 agctaaagaa tcagtattca aaggaagaat ataataatct tatcgctcaa ataaatgagc
    14881 gttataaaga tccaaagcaa ccaaaggcga aaggttattc tgatgatgca gcccaacgaa
    14941 tgattgatca tctgaatcaa cagaatgcgt tactaagttc acaagctgag ttgaccgtta
    15001 aattaagttc ctctgaacag gaactggtta agtggcgtca gcggattgcc gacctagagt
    15061 cacgaccatc atcgaaatta acccaggatc agaaatcgct tctcttacac cgggaagaaa
    15121 taaccgcgtt gatggagaaa aatgttgcga ttgaaaagaa taacaggcta atcaaggaat
    15181 ccgccgaaat aacagcatgg cgtgattcat tgcaggcttc gattgataat cgtcagcagg
    15241 ggtatgatat tcagattgct ggttatgggg ttggcgataa aaatcagcaa cgccagcagg
    15301 aattactgcg gattgaacat ggatataaca atcagcgtct gcaacttgaa cgtgactatg
    15361 cagataaatc ccgtggaatg tcagatcatg tttttcaaga gaaaatgcag gctctgaatg
    15421 atgctctgga acgggaaaaa gaaattgtca gacagaaaaa cgagcagctc gatattcagg
    15481 caggagactg ggttagtggt gcctcccagg gattcaataa ctggctggat gacactaagg
    15541 atatcagtga gcagataaaa tcaaccacga ctcagatgtt tgatgggatg accgatgcgc
    15601 tgggtgattt tgtcacgaca ggcaaggcaa attttcgttc tttcgctact tccgtgattt
    15661 cggatcttag tcgaatagca ttaaaggctt caattactgg gattttcgac agcattagta
    15721 acagttcttc tggggggatt ttaggaacta tcgggagtgc tattagtaaa tttattccga
    15781 atgcaaaggg cggtgtttat gagtctccgt cattgagcac gtattcgaac ggtatttatg
    15841 attccccgca attttttgct tttgcaaaag gggctggtgt ttttggtgag gctggaccgg
    15901 aagccattat gccattaaca cgaacttccg atggttctct tggtgtcaga gctattaata
    15961 gtaaaagtgg taatggaggc ggagatatta cctatgcccc tgtataccaa atcactattc
    16021 aaaatgacgg ccagaatgga gagattggcc ctcaggcaat aaaagcactt atggggatgg
    16081 ttgatcagcg ggtgcagggc aatctgttaa atatgcgacg tgatggggga atgttaagtg
    16141 gctaatacgg aagaatttca ctggttacca gaggatggaa tgaaaacaga aaataaacca
    16201 tcgataaaaa ctgtaagatt tggtgatggt tatgagcaac gaagtccaaa tggacttaat
    16261 cattctctgc gcgttttcac ctgtgatttc aaagttgagg cgaatgaacg tgattcattt
    16321 gaaaaatttt tagcccggca tgaaggctat aaatcttttt tttggcgccc gcctggtatt
    16381 aacagaaaaa tcagagtggt gtgtcgaacg tggtcagcga cagaacatat cacctatacc
    16441 gatttttcgt gtcagtttga cgaagtggtg atctgatgca ggacatacaa caggaaacgc
    16501 tgaatgaatg cattcgtgcg gagcagtcgg ccagcgtggt gctctgggaa atcgacctga
    16561 ccgaggtagg cggggatcgc tactttttct gcaatgagca gaacgagaaa ggagagtctg
    16621 tcacctggca gagtcggcag tatcaggcct atcccattca ggggagtgga tttgagatga
    16681 acggcaaagg tgccagtgca aggccaacgc tgaaagtctc taatctgcac ggcatggtca
    16741 ccgggatggc ggaagacctg cagagtctgg tcggcggaac ggtggtcagg cgtaaggttt
    16801 acgcccgttt tctggatgcg gtgaacttcg tcaacggaaa cagtgacgcc gatccggagc
    16861 aggaggtgat cagccgctgg cgtattgagc agtgcagcga actgagcgcg gtcagtgcct
    16921 cctttgtact gtccacgccg acggaaacgg acggcgctgt ttttccgggg cgcatcatgc
    16981 ttgctaatac ctgcacctgg acctatcgcg gtgatgagtg cggttatcac ggtccggtgg
    17041 tcgcggatga atatgaccag ccgacatccg aaatcacgaa ggataaatgc agcaaatgcc
    17101 tgagtggctg taagttccgc aataatgtcg gcaactttgg cggcttcctt tccattaaca
    17161 aactttcgca gtaaatctca tgacagagac agaatcagcg attctggcgc acgcccggcg
    17221 atgtgcgcca gcggagtcgt gcggcttcgt ggtgagagcg ccggaggggg aaagatattt
    17281 tccctgcgtg aatatttccg gtgagccgga ggtgtatttc cggatggctc cggaggactg
    17341 gctgcaggca gagatgcagg gtgagattgt ggcactggtc cacagtcatc ccggtggtct
    17401 gccctggctg agtgaggttg accggcggct gcaggtgcag agtgatttgc cgtggtggct
    17461 ggtctgccgg ggggcgattc acaagttccg ctgtgtgcca catcttaccg ggcggcgctt
    17521 tgagcacggg gtgacggact gttacacgat gttccgggat gcataccatc tggcgggaat
    17581 tgagatgccg gattttcatc gcggggatga ctggtggcgt aacggccaga atctttacct
    17641 ggacaatatg gaggcctccg gcttttatcg ggtgcccctg tcctctgcac aggcgggcga
    17701 tatcctgctg tgctgttttg gttcatcggt gccgaatcac gccgccattt actgtggtga
    17761 cggcgagctg ctgcaccata ttcctgaaca actgagcaaa cgagagaggt ataccgacaa
    17821 atggcagcga cgcacacact ccctctggcg tcaccgggca tggcgcgcat ctgcctttac
    17881 ggggatttac aacgatttgg ccgccgtatc gaccttcgtg tgaaaacggg ggctgaagcc
    17941 atccgggcac tggccacaca gctcccggcg tttcgtcaga aactgaatga cggctggtat
    18001 caggtgcgca ttgccgggcg tgatgcaggc gaaaccgaat tatctgcccg tcttaatgaa
    18061 tcgctggcaa atggtgccgt gatccacatc gtgccgcgtc tggcgggagc taaaagtggc
    18121 ggtgtgtttc aggcggtgct gggtgcggcg ctgattgcgg tggcatggtg gaaccctgtg
    18181 ggctggctgg gtgccgcggc tgtatcgggc atgtatgcgg caggggccag tatgatcctg
    18241 ggcggtgtgg cgcagatgct ggcaccgaaa gccaggacgc ccacggcagc cagtacagat
    18301 aacggcaaac agaacaccta tttctcgtca ctggataaca tggttgccca gggcaatgtt
    18361 ctgccggttc tgtacggtga aatgcgcgtg gggtcacgcg tggtttctca ggagatcagc
    18421 acggcagacg aaggggatgg tggtcaggtt gtggtgattg gtcgctgatg caaaatgttt
    18481 tatgtgaaac cgcctgcggg cggttttgtc gtttatggag cgtgaggaat gggtaaaggc
    18541 agcagtaagg ggcatacccc gcgcgaagcg aaggacaacc tgaagtccac gcagctgctg
    18601 agtgtgatcg atgccatcag cgaagggccg gttgaaggtc cggtggatgg attaaaaagc
    18661 gtgctgctga acagtacgcc ggtgctggac agtgagggga ataccaatat atctggcgtc
    18721 acggtggtgt tccgggccgg tgagcaggag cagtcaccgc cggagggatt tgaatcctcc
    18781 ggctccgaga cggtgctggg tacggaagtg aaatatgaca cgccgatcac ccggaccatc
    18841 acgtcggcaa acatcgaccg tctgcgcttt accttcggtg tacaggcact ggtggaaacc
    18901 acctcaaagg gggacaggaa tccatcggaa gtccgcctgc tggttcagat acagcgtaac
    18961 ggtggctggg tgacggaaaa agacatcacc attaagggca aaaccacctc gcagtatctg
    19021 gcctcggtgg tggtggataa cctgccgccg cgcccgttta atatccggat gcgcaggatg
    19081 acgccggaca gcaccacaga ccagctgcag aacaaaacgc tctggtcgtc atacaccgaa
    19141 atcatcgatg tgaaacagtg ctacccgaac acggcactgg ttggcgtgca ggtggactcg
    19201 gagcagttcg gcagccagca ggtgagccgt aattatcatc ttcgcgggcg cattctgcag
    19261 gtgccgtcga actataaccc gcagacgcgg caatacagcg gtatctggga cggaacgtta
    19321 aaaccggcat acagcaacaa catggcctgg tgtctgtggg atatgctgac ccatccgcgc
    19381 tacggcatgg gaaaacgtct tggtgcggcg gatgtggaca aatgggcgct gtatgtcatc
    19441 ggccagaatt gcgaccagtc agtgccggac ggctttggcg gcacggagcc acgcatcacc
    19501 tgtaatgcgt acctgaccac acagcgcaag gcgtgggatg tgctcagtga tttctgctcg
    19561 gcgatgcgct gtatgccggt atggaacggg cagacgctga cgttcgtgca ggaccgacca
    19621 tcagataagg tgtggaccta taaccgcagt aatgtggtga tgccggatga tggcgcgccg
    19681 ttccgctaca gcttcagcgc cctgaaggac cgccataatg ccgttgaggt gaactggatt
    19741 gacccggata acggctggga gacggcgaca gagcttgtag aggacacgca ggccatagcc
    19801 cgttacggtc gtaacgtcac gaagatggat gcctttggct gtaccagtcg ggggcaggca
    19861 caccgcgccg ggctgtggct gattaaaaca gaactgctgg aaacgcagac cgtggatttc
    19921 agcgtcggcg cagaagggct tcgccatgta ccgggcgatg ttattgaaat ctgtgatgat
    19981 gactatgccg gtatcagcac cggtggtcgt gtgctggcgg tgaacagcca gacccggacg
    20041 ctgacgctcg accgtgaaat cacgctgcca tcctccggta ccacgctgat aagcctggtt
    20101 gacggaagtg gcaatccggt cagcgtggag gttcagtccg tcaccgatgg cgtgaaggta
    20161 aaagtgagcc gtgttcctga tggcgttgcc ggatacagcg tgtgggggct gaagctgccg
    20221 acgctgcgcc agcgactgtt ccgctgcgtg agtatccgtg agaacgacga cggcacgtat
    20281 gccatcactg ccgtgcagca tgtaccggag aaagaagcca tcgtggataa cggggcgcac
    20341 tttgacggtg accagagcgg cacggtgaat ggtgtcacgc cgccagcagt gcagcacctg
    20401 actgccgaag tcaccgcaga cagcggggaa tatcaggtac tggcgcgatg ggacacgccg
    20461 aaggtggtga agggggtgag ctttatgctt cgcctgaccg tggcagcgga tgacggcagt
    20521 gagcggcttg tcagcacagc ccggacgacg gaaaccacat accgcttcag gcaactggcg
    20581 ctggggcgtt acacgctgac agtccgggca gtaaatgcgt gggggcagca gggcgatccg
    20641 gcgtcggtat cgttccggat tgccgcaccg tcagcgccgt cgcggattga gctgactccg
    20701 ggctattttc agatcaccgc cacgccgcat cttgccgttt atgacccgac ggtacagttt
    20761 gagttctggt tctcggaaaa gcggattgcg gatatcaggc aggttgaaac cacagcccgc
    20821 tatcttggca cggcgctgta ctggatagct gccagtatca atatcaagcc gggccatgat
    20881 tattattttt acatccgcag tgtgaacacc gttggcaaat cggcattcgt ggaggctgtt
    20941 ggccagccga gtgatgatgc atccggctat ctggattttt tcaaaggaga gatagggaaa
    21001 acccatctgg ctcaggagtt gtggacgcag attgataacg gtcagcttgc gcctgacctg
    21061 gcggaaatca gaacgtccat cacggatgtc agtaatgaaa tcacgcagac cgtcaataag
    21121 aaactggaag accagagtgc agcgatccag cagatacaga aggttcaggt tgatacaaat
    21181 aataacctga acagcatgtg ggctgtgaag ctgcagcaga tgcaggacgg acgcctttat
    21241 atcgcgggta ttggtgccgg tattgaaaac acccctgacg gcatgcagag tcaggtgctg
    21301 ctggcggcag acaggattgc gatgattaat cctgcgaatg gcaacacaaa gccgatgttt
    21361 gttgggcagg gcgatcagat atacatgaat gaagtgttcc tgaaatatct gacggctccc
    21421 accattacca gcggcggtaa tcctccggca ttttccctga caccggacgg gcggctgacg
    21481 gcgaaaaatg ccgatatcag cggtaacgtg aatgcgaact ccgggacgct caacaacgtc
    21541 acgattaacg agaactgtcg ggttctggga aaactgtccg ccaaccagat tgaaggtgat
    21601 ctcgttaaaa cagtgggcaa agctttcccc cgggactccc gtgcaccgga gcggtggcca
    21661 tcaggaacca ttaccgtcag ggtttatgac gatcagccgt ttgaccggca aattgttatc
    21721 ccggcggtgg cattcagtgg cgctaaacat gagcaagatc atactgatat ctactcctca
    21781 tgccgtctga tagtacgaaa aaacggtgct gaaatttata accgaacggc tctggataat
    21841 acgctgatat atacgggtgt tattgatatg cctgcaggca gtggtgtcat gacactggaa
    21901 ttttctgtat cggcatggct ggtaaatggc tggtatccca cagcaagtat cagcgatttg
    21961 ctggttgtgg tgatgaagaa agccactgca ggcatcacga ttagctgaat tttataaccc
    22021 agatacgggc accagaaatg gtgccttttt tattgcagaa aagcgagagg taattatgcg
    22081 taaattatgt gctgttattc tgtctgcagt agtctggctg gttgccgctg gtatgccagc
    22141 gagcgcagca gagcatcagt ccacactaag cgccgggtat cttcagaccc atactgatat
    22201 gccaggcagt gatgacctga agggcattaa cgtgaaatac cgttatgagt ttacggacac
    22261 actggggctg gtgacgtcat tcagctatgc aggagacaag aatcgccagc ttacccgtta
    22321 cagcgatacc cgctggcatg aagattccgt gcgtaaccgc tggttcagcg tgatggcggg
    22381 gccgtctgtg cgcgtgaatg aatggttcag cgcgtatgcg atggcgggta tggcttacag
    22441 ccgtgtttcg actttctccg gagattatat ccgcgtaact gacaacaagg ggaaaacgca
    22501 cgatgtgctg accggaagtg atgacggtcg ccacagcgac acgtctctgg catggggggc
    22561 tggcgtgcag tttaacccga ccgaatccgt ggccattgat attgcttatg aaggttccgg
    22621 cagtggcgac tggcgcactg acggtttcat cgttggtgtc ggttataaat tctgattagc
    22681 caggtaacac agtgttatga cagcccgccg gttcaggcgg gcttttttgt ggagtggata
    22741 tggcagcagt aaaaatctca ggtgtgctga aagatggtgc gggaaaacca atacagaact
    22801 gcactattca actgaaggca aagcgtaaca gcaccacggt actggtcaac acagtggcct
    22861 ctgaaaatcc ggatgaagcc gggcgttaca gcatggatgt tgagtatggc cagtacagcg
    22921 tcaccctgct ggttgaaggt tttccgcctt cacatgccgg aaccattacc gtctatgaag
    22981 gttccagacc aggtacgctg aatgattttc tcggtgccat gacggaagat gatgtcatgc
    23041 cggaggcatt gcgtcgtttt gaggaaatgg tggaagaagc ggcacgcaac gctgaagccg
    23101 cctctcagag cgcagcggcg gcaaagaaat ccgaaactgc agcggcatca tcgaagaacg
    23161 cggcgaaaac ctcagaaacg aatgcagcta acagcgcaca ggcggcagcg gcctcacaga
    23221 ctgcatcggc aaactccgcg actgcagcca aaaaatcaga aaccaacgcg aaaaacagtg
    23281 agacagccgc aaagacgagc gaaaccaacg caaagtccag ccagacggca gcgaagacca
    23341 gcgaaacgaa tgccaaagcc agtgaaactg cggcgaaaaa cagccaggct gcagcagctg
    23401 aaagcgagag cgcagcagcc ggttctgcga cttcagcagc cggatcagca actgctgcgg
    23461 ctaacagcca gaaagctgcg aagacgagtg aaactaacgc aaagtccagc cagacagcag
    23521 cgaagaccag cgaaacgaat gccaaagcca gcgaaactgc ggcgaaaaac agtcaggctg
    23581 cagcagccga aagcgagagt gctgcagctg gctctgcaag cgcggcggct gcttctgcca
    23641 ctgcatcagc caacagccaa aaagcagcaa aaaccagtga aaccaacgca aaggtgagcg
    23701 aaacagcggc tgcgaactca gcgaaagcat cggcagcaag ccagacggca gctaaagcaa
    23761 gcgaagatgc agccagagag tacgcaagcc aggctgcgga gccgtataaa tatgtcttac
    23821 agccgttgcc tgatgtgtgg ataccgttta acgattcact ggatatgatt accgggtttg
    23881 ctcctggata taagagcatc acagttggtg acgatgttat tgcattgccg tctgaaaagg
    23941 ttgtttcatt taccagggcg tcaactgcaa cgtatataga taagtctggg tgttttgctg
    24001 aatcagcgat aaatgaacca cgttttgaaa aagatggtct gctcattgaa ggtcagagaa
    24061 cgaatacttt ttcttatacg aatacaccag tatcgtggaa ctatgacact gctaacttaa
    24121 ctattaccac gggagttgat gagtatggtt tcagttatgg tttgtttggc gttaaagaaa
    24181 catccacaac tgaaagggcg acattaattt ctactggata taccagggtt atttcagttt
    24241 cggcaaatga atcagttact ttatcctgca gagttaaaaa agtaagtggg gatggtatta
    24301 tcacgttgcg tccaagaata tcatatgtta acgacgatgg ctcaagtaac acactgaccg
    24361 ctggtgcata tattgattgc gagactggcg atatgttgag ttattctgga ggtgaggcgg
    24421 caacttataa catattcaga gagtctaatg gatggattcg tgttgagttt acctacaaat
    24481 caccagaagc aaaaaatatg tatggacgtt ttgagtttgg agcacatcaa cgatcaatca
    24541 agtctggcga taaattaatg ttaacaaccc ctcaattcga aaagggacta aacgcctcat
    24601 cttttatcat cacaacagag gtcggtgcca cgagagcaag tgaccaggta atcataccta
    24661 tacctttcaa ctgggcaacg ccaccagtta gtgttctcat ggaagttaat gttaattggg
    24721 attctgaaat gcctaattta gaaggctctg cgcgtttgct taatatctca attacagggg
    24781 cgacgactga agtttctgat gaaagttata tgtattttgg ttttaccact cgtggtaaaa
    24841 ggctaattat caccaatggc aaaggaacaa aaacagaata taaagcatat gggaatagag
    24901 agaaaaggaa atttgttact ggctttaagt ttacagaaga taaacagttg caggttgttg
    24961 ttgatggaat tttaggtagc agctccccgt ctctgcatac attgcaacgt tatactgccg
    25021 gtaatattaa tatcggtgga caatcatcca gtggcaacag acacctgttc ggtcatgtga
    25081 aaaatttacg catttggcat aaagaattaa ctgaggcaca aatgggggag agtattaaat
    25141 gaaagattta acactcaaat ttgccgacag ggccgacttt tcggccttta tggagagtat
    25201 tggctattat gatgacgagt cgatgcagga tgatattctt attgacgtga taggtaacgt
    25261 gtacaaagaa accggagaac taactgaaga tggcgaaccg gtatgtgtta aggaagacgg
    25321 atattttgta aacgtgcgca tcattaatga tgcaaaaaaa tcgtcaatat tcgatgaata
    25381 cgtggttgct gttgagcatc aacttcgtgg ctggatgtga ggaagaaaaa tggctacatc
    25441 gacagtaatt cctgaagaca tcaaaacgct aaagggagat gtcagtaaaa ctaaggaaga
    25501 tatttcctca attaacggaa aagtatcaac gcttcagatt gatatgacca gtgcaaagca
    25561 ggatatcagc accagataca caaaaactga agttgataat aagctgaaaa acaaagtgga
    25621 agcgaacgat ctggaaagcg gtcgttatgg cggtgatttt tacccgctga caggtcgtga
    25681 agcgttttat ttatggaatt tggccacgac aacagcggcg gcaaaccttt atcttaatcc
    25741 tgaccctgca atttcgtctg tactgcggtc aacatcgtct attcgctata aacattcagt
    25801 agagacgata gattcagagc acgccgatct cattttcaag atgcgccctg tgtggtacag
    25861 gtcgcaatgc gaaaatgaca ggcgtgactg gggattctac gggttgattg ccgaggaagt
    25921 tggagaaatt gcccctcagt ttgtacactg gcgaccagct aacgagaatg atgctcctga
    25981 agctatttcc agcaatggcc ttgttgccga aggtgtaatg tacgaacgtc tggttgttcc
    26041 actgattgac catatccaga aactgactga aagagttgaa gaacttgagt cagaattaaa
    26101 gttgttatca acttcctgaa gcgatattgg ataaaggagg agtaatggat ataacacctt
    26161 tccttcacgc gctttgtgct gtggctgcgc aggtactggt tggtcttttt accgggaact
    26221 gggcttacgg ggcgatagcc ggttgtacgt tcttcattgc gcgtgaacac acccaggcag
    26281 aatatcgctg gattgaaatg ttcgggcatg gcaagcgaat gaatatgccg tggtggggcg
    26341 gttttgatcc gcgcgcgtgg gatgtggcaa gcctgatgga ttttgctgtg ccggtggtgg
    26401 cgtgtctgct ggtctggctg ttggttaatc gtgggtgaaa aaaggtgagc tgtatatgca
    26461 acggaggaag aaacctcgtt gctggaagcc tggaagaagt atcgggtgtt gctgaaccgt
    26521 gttaatacaa caactgcacc ggatattgaa tggccaacag tacccattat cgaatagtgc
    26581 attacgaact gcaggatatt atatgggata atattctgca gtttattggg gctgtatttt
    26641 aaaaaggtgt ttattttgag tgaattatca ccttgttttt tccggttctt ttggcttgat
    26701 ataacgcttt atcggcattg actaacattt cagatggact gaggttattc tcttttgaag
    26761 taaagcaacc aatactgaca gttattttct caggtccatg cgataataat ttttccgagc
    26821 attgctcttc aatgtttttt ctgattcgct ctgcaattga tagcaagagc ttttgagtat
    26881 tacctttaat gataataccg aactcctcac cgcctaagcg ccctaatata tcctctttcc
    26941 tgatggattt tttattattc tggtaaccat tactatgact tgatcaccca tatgatgccc
    27001 ccatttgtca ttgatgc
//
LOCUS       DACBVI010000053        26751 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.053, whole genome shotgun sequence.
ACCESSION   DACBVI010000053 DACBVI010000000
VERSION     DACBVI010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.053"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            8..772
                     /locus_tag="IU325_10835"
     CDS             8..772
                     /locus_tag="IU325_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HAP5809517.1"
                     /translation="MKNTKLLLAIAASAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGAASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFELLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            945..1628
                     /locus_tag="IU325_10840"
     CDS             945..1628
                     /locus_tag="IU325_10840"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP5809518.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            1739..3412
                     /gene="rpsA"
                     /locus_tag="IU325_10845"
     CDS             1739..3412
                     /gene="rpsA"
                     /locus_tag="IU325_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP5809519.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            3572..3856
                     /gene="ihfB"
                     /locus_tag="IU325_10850"
     CDS             3572..3856
                     /gene="ihfB"
                     /locus_tag="IU325_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HAP5809520.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            4063..6327
                     /locus_tag="IU325_10855"
     CDS             4063..6327
                     /locus_tag="IU325_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HAP5809521.1"
                     /translation="MKITTVGVCIICGIFPLLVLPQLPGTLTLAFLTVFACVLAFIPV
                     KAVRYIALTLLFFLWGISAAKQILWAGETLTGATQDAIVEITATDGMTTHYGQVTHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGSFDSQRYAIAQHQPL
                     TGRFLQASVIEPDCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPAFRTVVALAMWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLTFSPWFLLVVWRLNAWRTLPAMCVAGGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEAPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYKWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            6364..8112
                     /gene="msbA"
                     /locus_tag="IU325_10860"
     CDS             6364..8112
                     /gene="msbA"
                     /locus_tag="IU325_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HAP5809522.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            8109..9095
                     /gene="lpxK"
                     /locus_tag="IU325_10865"
     CDS             8109..9095
                     /gene="lpxK"
                     /locus_tag="IU325_10865"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HAP5809523.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVIWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCLPFAEGNWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            9132..10364
                     /gene="ycaQ"
                     /locus_tag="IU325_10870"
     CDS             9132..10364
                     /gene="ycaQ"
                     /locus_tag="IU325_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein YcaQ"
                     /protein_id="HAP5809524.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSNFRLIRH
                     RMLAPEKMGWKYKNAWMQEHEAEIAQLIQHIHDKGPVRSADFEHSRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLERC
                     PQGLFTDCRTGWEIDPVA"
     gene            10416..10598
                     /locus_tag="IU325_10875"
     CDS             10416..10598
                     /locus_tag="IU325_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HAP5809525.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            10595..11341
                     /gene="kdsB"
                     /locus_tag="IU325_10880"
     CDS             10595..11341
                     /gene="kdsB"
                     /locus_tag="IU325_10880"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HAP5809526.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            11495..12388
                     /locus_tag="IU325_10885"
     CDS             11495..12388
                     /locus_tag="IU325_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HAP5809527.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12365..13144)
                     /gene="elyC"
                     /locus_tag="IU325_10890"
     CDS             complement(12365..13144)
                     /gene="elyC"
                     /locus_tag="IU325_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HAP5809528.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            13280..14065
                     /gene="cmoM"
                     /locus_tag="IU325_10895"
     CDS             13280..14065
                     /gene="cmoM"
                     /locus_tag="IU325_10895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HAP5809529.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            14062..15384
                     /gene="mukF"
                     /locus_tag="IU325_10900"
     CDS             14062..15384
                     /gene="mukF"
                     /locus_tag="IU325_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HAP5809530.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            15365..16069
                     /gene="mukE"
                     /locus_tag="IU325_10905"
     CDS             15365..16069
                     /gene="mukE"
                     /locus_tag="IU325_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HAP5809531.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            16069..20529
                     /gene="mukB"
                     /locus_tag="IU325_10910"
     CDS             16069..20529
                     /gene="mukB"
                     /locus_tag="IU325_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HAP5809532.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVLPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            20790..22637
                     /gene="ldtD"
                     /locus_tag="IU325_10915"
     CDS             20790..22637
                     /gene="ldtD"
                     /locus_tag="IU325_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAP5809533.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            22818..23366
                     /locus_tag="IU325_10920"
     CDS             22818..23366
                     /locus_tag="IU325_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HAP5809534.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            23393..24040
                     /gene="gloC"
                     /locus_tag="IU325_10925"
     CDS             23393..24040
                     /gene="gloC"
                     /locus_tag="IU325_10925"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HAP5809535.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVALQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(24091..25281)
                     /locus_tag="IU325_10930"
     CDS             complement(24091..25281)
                     /locus_tag="IU325_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP5809536.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(25466..26539)
                     /gene="ompF"
                     /locus_tag="IU325_10935"
     CDS             complement(25466..26539)
                     /gene="ompF"
                     /locus_tag="IU325_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HAP5809537.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKDNGVNSYGGNGDKTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQKGNK
                     TRLAFAGLKFADAGSIDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERAGIPERSNGDGVGGSISYEFEGFGIVGAYGAAD
                     RTDAQEAEFRGQGKKAEQWATGLKYDANNIYLAANYGETRNATPIEGGFANKTQDVLL
                     VAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVDYIINQ
                     IDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         6475 a         6585 c         7262 g         6429 t
ORIGIN      
        1 gattcacatg aagaatacta aattactgct ggcgattgcg gcctctgcag cattactgac
       61 agggtgtcaa aatacccacg gtattgatac caatatggct atcagctcag gtttaaatgc
      121 ctataaagca gcaacgttaa gcgatgctga tgcaaaagcg attgccaatc agggctgtgc
      181 cgaaatggac agcggcaatc aagtcgcaag taaatccagc aagtacggta aacgtctggc
      241 aaaaatcgcc aaagcattgg gtaacaatat taatggcacg ccggtcaact ataaggttta
      301 tatgaccagc gacgtcaacg catgggcgat ggcgaacggc tgtgttcgtg tctacagtgg
      361 cctgatggac atgatgaacg acaacgaaat tgaaggcgtt ctgggtcatg aactgggcca
      421 cgtcgcgttg ggtcactcgc tggctgaaat gaaagcttct tatgcgatcg ttgccgcacg
      481 tgatgcgatt tccgctacca gcggtgcggc ttcccagctt tctcgctcac aattaggtga
      541 tatcgcagaa ggcgcgatca atgctaaata ctcccgcgat aaagagtccg aagcagatga
      601 tttctccttt gaactgttga agaaacgtgg catcagcacc caggggctgg ttggcagctt
      661 tgaaaaattg gccagcctgg atggcggtcg tacccagtcc atgtttgact ctcacccacc
      721 atcaacagag cgtgcgcaac acatccgtga tcgtatcgcc tctggtaagt aaatcattgt
      781 catctttcgg gctggtcttc tgccagcccg ctataattgc gcaataaatc cccatctgaa
      841 tacagacaaa actggttttt gcacacaacg ttaacgattt gtggcgtcgg cgcgtataat
      901 gcgcgcggtt atgttaacgg tacgcctgtt ttaaggagat aaagatgacg gcaattgccc
      961 cggttattac cattgatggc ccaagcggtg cagggaaagg caccttgtgt aaggctatgg
     1021 cggaagcgtt gcaatggcat ctgctggact cgggtgcaat ttatcgcgta ctggcactgg
     1081 cggcattaca tcaccatgtt gatgttgcat cggaagatgc gctcgtgccg ctggcatccc
     1141 atctggatgt acgttttgtg tcgaccaatg gcaatctgga agtgatcctc gaaggggaag
     1201 atgtcagcgg cgaaattcgt actcaggaag tggcgaatgc tgcttcacaa gtcgcggcat
     1261 tcccacgcgt tcgtgaagca ttattgcgtc gccaacgcgc gtttcgcgaa ttaccaggct
     1321 tgattgccga tggccgcgat atgggaacgg tggtattccc tgatgcgcca gtgaaaattt
     1381 tccttgacgc ctcctcggaa gaacgtgcgc atcgccgcat gctacagttg caggagaagg
     1441 gctttagtgt taactttgag cgccttttgg ccgagatcaa agaacgcgac gaccgcgatc
     1501 gtaaccgagc ggtagcgcca ctggttccgg cagccgatgc tttagtgttg gattccacca
     1561 ccttaagcat tgagcaagtg attgaaaaag cgctacaata cgcgcgccag aaattggctc
     1621 tcgcataagc gaccgaattt gcagtacccc cgttgcaatg gaatgacagc gggtatgtta
     1681 aacaacccca tccggcatgg agccaggtgg acgttaaata taaacctgaa gattaaacat
     1741 gactgaatct tttgctcaac tctttgaaga gtccttaaaa gaaatcgaaa cccgcccggg
     1801 ttctatcgtt cgtggcgttg ttgttgctat cgacaaagac gtagtactgg ttgacgctgg
     1861 tctgaaatct gagtccgcca tcccggctga gcagttcaaa aacgcccagg gcgagctgga
     1921 aatccaggta ggtgacgaag ttgacgttgc tctggacgca gtagaagacg gcttcggtga
     1981 aactctgctg tcccgtgaga aagctaaacg tcacgaagcc tggatcacgc tggaaaaagc
     2041 ttacgaagat gctgaaactg ttaccggtgt tatcaacggc aaagttaagg gcggcttcac
     2101 tgttgagctg aacggtattc gtgcgttcct gccaggttct ctggtagacg ttcgtccggt
     2161 gcgtgacact ctgcacctgg aaggcaaaga gcttgaattc aaagtaatca agctggatca
     2221 gaagcgcaac aacgttgttg tttctcgtcg tgccgttatc gaatccgaaa acagcgcaga
     2281 gcgcgatcag ctgctggaaa acctgcagga aggcatggaa gttaaaggta tcgttaagaa
     2341 cctcactgac tacggtgcat tcgttgatct gggcggcgtt gacggcctgc tgcacatcac
     2401 tgatatggcc tggaaacgcg ttaagcatcc gagcgaaatc gtcaacgtgg gcgacgaaat
     2461 cactgttaaa gtgctgaagt tcgaccgcga acgtacccgt gtatccctgg gcctgaaaca
     2521 gctgggcgaa gatccgtggg tagctatcgc taaacgttat ccggaaggta ccaaactgac
     2581 tggtcgcgtg accaacctga ccgactacgg ctgcttcgtt gaaatcgaag aaggcgttga
     2641 aggcctggta cacgtttccg aaatggattg gaccaacaaa aacatccacc cgtccaaagt
     2701 tgttaacgtt ggcgatgtag tggaagttat ggttctggat atcgacgaag aacgtcgtcg
     2761 tatctccctg ggtctgaaac agtgcaaagc taacccgtgg cagcagttcg cggaaaccca
     2821 caacaagggt gaccgtgttg aaggtaaaat caagtctatc actgacttcg gtatcttcat
     2881 cggcctggac ggcggcatcg acggcctggt tcacctgtct gacatctcct ggaacgttgc
     2941 aggcgaagaa gcagttcgtg aatacaaaaa aggcgacgaa atcgctgcag ttgttctgca
     3001 ggttgacgca gaacgtgaac gtatctccct gggcgttaaa cagctcgcag aagatccgtt
     3061 caacaactgg gttgctctga acaagaaagg cgctatcgta accggtaaag taactgcagt
     3121 tgacgctaaa ggcgcaaccg tagaactggc tgacggcgtt gaaggttacc tgcgtgcttc
     3181 tgaagcatcc cgtgaccgcg ttgaagacgc taccctggtt ctgagcgttg gcgacgaagt
     3241 tgaagctaaa ttcaccggcg ttgatcgtaa aaaccgcgca atcagcctgt ctgttcgtgc
     3301 gaaagacgaa gctgacgaga aagatgcaat cgcaactgtt aacaaacagg aagatgcaaa
     3361 cttctccaac aacgcaatgg ctgaagcttt caaagcagct aaaggcgagt gattctctga
     3421 ctcttcggga tttttattcc gaagtttgtt gagtttactt gacagattgc aggtttcgtc
     3481 ctgtaatcaa gcactaaggg cggctacggc cgcccttaat caatgcagca acagcagccg
     3541 cttaatttgc ctttaaggaa ccggaggaat catgaccaag tcagaattga tagaaagact
     3601 tgccacccag caatcgcaca ttcccgccaa gacggttgaa gatgcagtaa aagagatgct
     3661 ggagcatatg gcctcgactc ttgcgcaggg cgagcgtatt gaaatccgcg gtttcggcag
     3721 tttctctttg cactaccgcg caccacgtac cggacgtaat ccgaagactg gcgataaagt
     3781 agaactggaa ggaaaatacg ttcctcactt taaaccaggt aaagaactgc gcgatcgcgc
     3841 caatatttac ggttaagttt ttactcaaac ttgaacgcga gaaaagcacc tgtcgggtgc
     3901 ttttttcatt tctctaatct ggaactggaa gctgcctcgc agagttttga acagttttca
     3961 ccctctcgtt aaattcttct gaacgtgcct cggggaacgc aaaactccca ccagacaacc
     4021 gctcaacaaa gttgcacact ttccataaat acggaggggt gcatgaaaat aacgacagtc
     4081 ggcgtatgca taatttgcgg aatttttccg ttgctggttt tgccccaatt gcctgggaca
     4141 ttaacccttg cgtttctgac tgtcttcgcc tgcgtactgg catttattcc tgttaaagcc
     4201 gtccgttata tcgcgctgac gttgctgttt tttctttggg gcatatcagc ggcaaagcaa
     4261 attttgtggg caggagaaac cttaactggc gcgacgcagg atgcaatagt tgagatcact
     4321 gctactgacg gcatgaccac tcattacggt caagtcactc atctacaagg tcgacgtata
     4381 ttccctgcgc caggcctcgt gctgtatggc gaatatctcc cgcaagcggt ttgtgccgga
     4441 caactatggt caatgaaact caaagttcgt gcagttcatg gccaacttaa tgatggcagc
     4501 tttgatagcc agcgttatgc cattgcccag catcagccgc tgaccggccg ctttctgcag
     4561 gcaagtgtca ttgaaccgga ttgtagcttg cgtgcacagt atctggcgtc attacaaaca
     4621 acgctgcaac cctatccgtg gaatgcggtt attcttggtt taggtatggg ggaacggtta
     4681 tccgttccca aagaaatcaa aaatatcatg cgcgatactg gaacggcgca tttaatggcg
     4741 atatcgggat tgcatatcgc ttttgcggcg ttgctggctg ccggactcat tcgcagtgga
     4801 caaatttttc tgcctgggcg ctggatccac tggcaaatgc cattaattgg cggaatctgc
     4861 tgtgctgctt tttatgcctg gctgacggga atgcaacctc ctgcatttcg taccgtggtg
     4921 gcgcttgcca tgtggggaat gcttaagtta agtgggcgac aatggagtgg ctgggatgta
     4981 tggatatgtt gtctggcggc aattttgctg atggatcctg ttgccattct ctcgcaaagt
     5041 ttatggctct ctgccgctgc ggtcgcggca ctgatttttt ggtatcagtg gtttccctgt
     5101 cctgagtggc agctgccgcc ggtattgcgt gcggttgttt ccctcatcca tctgcaactg
     5161 ggaatcaccc tcctgcttat gcccgtgcaa atcgtcatat ttcatggcat tagtctgacc
     5221 tcgtttattg caaatctgtt tgcaattccc ctggtgacat ttatcacggt tccattgatc
     5281 ctcgccgcga tggttgtgca tttaagcggg ccgttaatcc tggaacaggg gttatggttt
     5341 cttgccgacc ggtctttggc tttacttttc tgggggttaa agagtttgcc ggaagggtgg
     5401 atcaacattg ctgaacgttg gcaatggcta acattttccc catggttctt actggtggta
     5461 tggcgattaa acgcctggcg aacgttgcca gcaatgtgtg tggctggagg cttgctgatg
     5521 tgctggccgc tgtggcaaaa acctcgacct gacgagtggc aagtgtacat gcttgatgtc
     5581 gggcaagggc tggcaatggt cattgcgcga aacggcaaag cgattctcta tgacacggga
     5641 ctggcctggc ccgaagggga tagtgggcaa caactgatta tcccctggct ccactggcat
     5701 aatcttgaac cggaaggcgt tattctgagt catgaacatc tggatcaccg gggagggctg
     5761 gactcaatat tgcacacatg gccgatgtta tggatcagaa gtccgttaaa ctgggaacat
     5821 catcagccct gtgtgcgtgg cgaagcgtgg caatggcaag gattgcgttt cagcgcgcac
     5881 tggcctttac aagctagcaa cgataaagga aataaccatt cctgtgtggt taaggttgat
     5941 gacgggacga atagcattct tctaaccggt gatattgaag cccccgctga acaaaagatg
     6001 ctaagccgtt actggcagca agtgcaggca acattgcttc aggtacctca ccatggcagt
     6061 aatacctcat catcgttgcc attaattcag cgagtgaatg gaaaagtggc actcgcatcg
     6121 gcatcgcgct ataacgcatg gcgattgccc tccaacaaag ttaagcatcg ctatcaacaa
     6181 caaggttata agtggcttga tactccacat cagggtcaag tgacggtcaa tttttcagcg
     6241 caaggctggc ggattagcag cctcagggag caaattttac ctcgttggta tcatcagtgg
     6301 tttggcgtgc cagtggataa cgggtagaat atgcggctat ttcaacaaat gctggttttt
     6361 tgaatgcata acgacaaaga tctctctacg tggcagacat tccgccgact gtggccaacc
     6421 attgcgcctt tcaaagcggg tctgatcgtg gcgggcgtag cgttaatcct caacgcagcc
     6481 agcgatacct tcatgttatc gctccttaag ccacttcttg atgatggctt tggtaaaaca
     6541 gatcgctccg tgctggtgtg gatgccgctg gtggtgatcg ggctaatgat tttacgtggt
     6601 atcaccagct atgtctccag ctactgtatc tcctgggtat caggaaaggt ggtaatgacc
     6661 atgcgtcgcc gcctgtttgg tcacatgatg ggaatgccag tttcattctt tgacaaacag
     6721 tcaacgggta cgctgttgtc acgtatcacc tacgattccg aacaggttgc ttcttcttct
     6781 tccggcgcac tgattactgt tgtgcgtgaa ggtgcgtcga tcatcggcct gttcatcatg
     6841 atgttctatt acagctggca actgtcgatc attttgattg tgctggcacc gattgtttcg
     6901 attgcgattc gcgttgtatc gaagcgtttt cgtaacatca gtaaaaacat gcagaacacc
     6961 atggggcagg tgaccaccag cgcagaacaa atgctgaagg gccacaaaga agtattgatt
     7021 ttcggtggtc aggaagtgga aacgaaacgc ttcgataaag tcagtaaccg aatgcgtctt
     7081 caggggatga aaatggtttc agcctcttcc atctctgatc cgatcattca gctgatcgcc
     7141 tctttggcgc tggcgtttgt tctgtatgcg gcgagcttcc caagtgtcat ggatagcctg
     7201 actgccggta cgattaccgt tgttttctct tcaatgattg cactgatgcg tccgctgaaa
     7261 tcgctgacca acgttaacgc ccagttccag cgcggtatgg cggcttgtca gacgctgttt
     7321 accattctgg acagtgagca ggagaaagac gaaggtaagc gcgtgatcga gcgtgcgact
     7381 ggcgacgtgg aattccgcaa tgtcaccttt acttatccgg gacgtgacgt gcccgcattg
     7441 cgtaacatca acctgaaaat tccggcaggg aagacggttg ctctggttgg acgctctggt
     7501 tcaggtaaat caaccatcgc cagcctaatc acacgttttt acgatattga tgaaggcgaa
     7561 atcctgatgg atggtcacga tctgcgcgag tataccctgg cgtcgttacg taaccaggtt
     7621 gctctggtgt cgcagaatgt ccatctgttt aacgatacgg ttgccaacaa cattgcttac
     7681 gcacggactg aacagtacag ccgtgagcaa attgaagaag cggcgcgtat ggcctacgcc
     7741 atggacttca tcaataagat ggataacggt ctcgatacag tgattggtga aaacggcgtg
     7801 ctgctctctg gcggtcagcg tcagcgtatt gccatcgctc gagccttgtt gcgtgatagc
     7861 ccgattctga ttctggacga agctacctcg gcgttggata ccgaatccga acgtgcgatt
     7921 caggcggcac tggatgagtt gcagaaaaac cgtacctctc tggtgattgc ccaccgcttg
     7981 tctaccattg aaaaggcaga cgaaatcgtg gtcgtcgagg atggtgtcat tgtggaacgc
     8041 ggtacgcata acgatttgct tgagcaccgt ggcgtttacg cgcaacttca caaaatgcag
     8101 tttggtcaat gatcgaaaaa atctggtctg gtgaatcccc tttgtggcgg ctattgctgc
     8161 cactctcctg gttgtatggc ctggtgagtg gcgcgatccg tctttgctat aaactaaaac
     8221 tgaagcgcgc ctggcgtgcc cccgtaccgg ttgtcgtggt tggtaatctc accgcaggcg
     8281 gcaacggaaa aaccccggtc gttatctggc tggtggaaca gctgcaacag cgcggtattc
     8341 gcgtgggggt cgtatcgcgg ggatatggtg gtaaggctga atcttatccg ctgttattgt
     8401 cggcagatac caccacagca caggcgggtg atgaacctgt gttgatttat caacgcactg
     8461 gcgcgcccgt tgcggtttct cccgtgcgtt ctgatgcggt aaaagccatt ctggcgcaac
     8521 accctgatgt gcagatcatc gtaaccgacg acggtttaca gcattaccgt ctggcgcgtg
     8581 atgtggaaat tgtcgttatt gatggtgtgc gtcgctttgg caatggttgg tggttgccgg
     8641 cggggccaat gcgtgagcga gcggggcgct taaagtcggt tgatgcggta atcgtcaacg
     8701 gcggtgtccc ccgcagcggt gaaatcccca tgcatctgct gccgggtcag gcggtgaatt
     8761 tacgtaccgg tacgcgttgt gacgttgctc agcttgaaca tgtagtggcg atggcgggga
     8821 ttggacatcc gccgcgcttt tttgccacgc tgaagatgtg cggcgtacaa ccggaaaaat
     8881 gtgtaccact ggccgatcat cagtctttga accatgcgga tgttagtgcg ttggtaagcg
     8941 ccgggcaaac gctggtaatg actgaaaaag atgcggtgaa atgcctgccc tttgccgaag
     9001 gaaattggtg gtatttgcct gttgacgcac agctttcagg tgatgaacca gcgaaactgc
     9061 ttgcgcaact aacctcgctg gcttctggca actagttacg ccgcggcagc gttcgattga
     9121 tggagtcatg aatgtcgctg ccgcacctct ctcttgctga tgcgcgtaat cttcacctcg
     9181 ccgcgcaagg cctgttaaac aaaccccgcc gtcgagcatc gttggaggat attccggcaa
     9241 cgatctcccg catgtccttg ctgcaaatcg ataccattaa tattgttgcc cgtagtccat
     9301 atctggtgct tttcagtcgt ctgggaaatt atcctgccca gtggctggat gagtctctgg
     9361 cgcgtggcga attaatggaa tactgggcgc atgaagcctg ctttatgccg cgtagcaact
     9421 ttcgtcttat tcgccaccgc atgctggcac ctgaaaaaat gggctggaaa tacaaaaacg
     9481 cctggatgca ggaacatgag gcggaaattg cacagttaat tcagcatatt catgataagg
     9541 ggccggtacg ttcagccgat ttcgagcatt ctcgtaaagg tgcaagcggc tggtgggaat
     9601 ggaagccgca taaacggcat ctggaaggtt tatttactgc cggaaaggtg atggtgattg
     9661 aacggcgcaa cttccagcgc gtttatgatt taacccaccg agtcatgcct gactgggatg
     9721 atgagcgcga tctcgtttcg caaacagaag cagaaatcat catgctggat aacagtgcgc
     9781 gtagcctggg aatattccgc gaacagtggc tggcagatta ctatcggctg aaacgtccgg
     9841 cactggcggc gtggcgcgaa gcgagggctg aacagcagca aatcattgct gtgcatgttg
     9901 aaaaattggg caatctttgg ctgcatgctg atttgctgcc gctactcgag cgtgcgctgg
     9961 ccggaaagct cactgcaacg cacagcgcgg tactttcgcc ttttgatcct gttgtctggg
    10021 atcgcaaacg cgcagagcag ctttttgatt ttagctaccg gctggagtgc tataccccag
    10081 cgccgaaacg ccagtatggc tattttgttc tgccgttatt acatcgtggg caattagttg
    10141 ggcgaatgga tgccaaaatg catcgccaga caggcatcct tgaagttatc tctctgtggt
    10201 tacaagaagg cattaaacca acgacaacgc tgcaaaaagg gttacgtcag gcgattactg
    10261 atttcgctaa ctggcagcag gcaacgcggg tgacattaga acgctgcccg caaggcctct
    10321 ttactgattg ccgcaccggc tgggaaatag accccgtcgc ataaatgatt atgataagat
    10381 ttatgcattc ataacccgat ccatctggag gaactatgga tcatcgtctg cttgaaatca
    10441 ttgcctgccc ggtttgcaac ggaaaacttt ggtataacca ggaaaaacaa gaacttattt
    10501 gcaaactgga caacctggct ttcccgctgc gcgatggcat tccagtgttg ctggaaacgg
    10561 aagcccgcgt gctgactgct gatgagagta aatcatgagt tttgtggtca ttattcccgc
    10621 gcgctacgcg tccacgcgtc tgcccggtaa accattggtt gatattaacg gcaaacccat
    10681 gattgttcat gttcttgaac gcgcgcgcga atcaggtgcc gagcgcatca tcgtggcaac
    10741 cgatcatgag gatgttgctc gcgctgttga agccgctggc ggtgaagtat gtatgacgcg
    10801 cgccgatcat cagtcaggaa cagaacgtct ggcggaagtt gtcgaaaaat gcgcattcag
    10861 cgacgacacg gtgatcgtta atgtgcaggg ggatgaaccg atgatccctg cgacaatcat
    10921 tcgtcaggtt gctgataatc tcgcccagcg tcaggtgggt atggcgactc tggcggtgcc
    10981 aatccacaat gcggaggaag cgtttaaccc gaatgcggtg aaagtggtcc tcgacgctga
    11041 agggtatgca ctgtacttct ctcgcgctac cattccttgg gatcgtgatc gttttgcaaa
    11101 aggccttgaa accgttggcg ataacttcct gcgtcatctt ggtatttatg gctaccgtgc
    11161 aggctttatc cgtcgttacg tcaactggca gccaagtccg ttagaacaca tcgaaatgtt
    11221 agagcagctt cgtgttctgt ggtacggcga aaaaatccat gttgctgtcg cccaggaagt
    11281 tcctggcaca ggtgtggata cccctgaaga tctcgagcgc gttcgcgctg aaatgcgcta
    11341 atttcacttc acgacactcc agccaatttt gggaggagtg tcgtaccgtt acgattttcc
    11401 tcaatttttc ttttcaacaa ttgatctcat tcaggtgaca tcttttatat tggcgctcat
    11461 tatgaaagca gtagctttta tgagggtaat ctgaatggaa cagctgcgtg ccgaattaag
    11521 ccatttactg ggcgaaaaac tcagtcgtat tgagtgcgtc aatgaaaaag cggatacggc
    11581 gttgtgggct ttgtatgaca gccagggaaa cccaatgccg ttaatggcaa gaagctttag
    11641 tacgcccgga aaagcccgac aactggcatg gaaaaccacc atgctggcaa gaagtggaac
    11701 tgtccgtatg ccgactattt atggtgtgat gacgcatgaa gaacaccccg gccccgatgt
    11761 cctgctactg gagcggatgc gtggtgtttc ggtggaggca ccagcccgaa caccagaacg
    11821 ctgggaacag ctcaaagatc aaatcgttga agccttactg gcctggcacc gtcaggacag
    11881 tcgcggttgc gtcggcgcgg tcgacaatac tcaggaaaat ttctggccct catggtaccg
    11941 gcaacatgtt gaagtgctat ggaccacgct caatcagttc aataacaccg gtctaacgat
    12001 gcaggataag cggatcctgt ttcgtactcg cgaatgtctt ccggcattat ttgaaggctt
    12061 taacgacaat tgtgtgctga ttcacggtaa cttctgttta cgcagcatgc tgaaagattc
    12121 gcgcagcgat cagttactgg cgatggtcgg gccgggacta atgctttggg caccgcgaga
    12181 atacgaactg ttccgactaa tggataattc tctggcggaa gatttgctct ggagttacct
    12241 gcaacgcgcg ccagtggcgg agtcgttcat ctggcggcgt tggttgtatg tgttatggga
    12301 tgaagttgcg caactggtta ataccggacg atttagtcgg cgcaacttcg atctggcatc
    12361 aaaatcactc ttgccgtggc tcgcctgacg gacctttcag ccactgccag atgcggccaa
    12421 gcgtttcata gccaacacga tcgctatgca tcaaccaaac cggagaaggg attgcccgtt
    12481 cccacggatt aagcggcgag tcgatggcca actgattcgc tggggctggt agcggattta
    12541 acccttcctg ctgaaaaaag atcatcgccc gcggcagatg tgatgcggag gtcaccagca
    12601 ggaaaggggc gtcaccaatc gcctgtttca ctgccgcagc ttcttcttcg gtatcttttg
    12661 gcaaatccag agtgataatt tgctcgcgcg gcacgcccag cgattgcgca actctggcac
    12721 ctacttccgc tgtactcacc gtattggttt ttgctacgcc tcccgtgaag atcagttttg
    12781 atcccggatt ttcgcgccat aagcgaatac cttcattcag gcgaggcaga ctgttattga
    12841 ttaaattcga gcttggtgcc cactgcgggt tccaggtata accacctccg agcaccacga
    12901 tatagtccac tttttgcgaa ttattccatg tcggataggt gctttcgatg ggacgcagca
    12961 ggcggtcggc taccggttgc aaactcaata acaagagcgc cagccaccca atgctaataa
    13021 aaatcttccc ggttttttga aaacggctaa accacagcag cgccaggcca gcgccaataa
    13081 tcaacaacat cagcggaagt ggcagcaaca tattgccaat cacttttttc agtgtaaaaa
    13141 gcatcctttc tggttccttt tttaaccata tagcaaggga tcgccagcga tattacacca
    13201 gacaggttca ttctcgccgt ggctgtgaca aaatagcgtc tttatcagaa aacgtcttag
    13261 ccaaccggtg gagaaagcaa tgcaggatcg caattttgat gatattgcgg aaaagttttc
    13321 ccgtaacatt tacggcacca ccaaagggca gcttcgacag gctattctgt ggcaggatct
    13381 cgatcgcgtg ctggcggaaa tgggcccgca aaaactgcgt gtgctggatg ctggcggtgg
    13441 agaagggcag accgcaatca aaatggccga gcgtgggcat caggtcattt tatgcgatct
    13501 ttctgcgcag atgatcgacc gcgcaaaaca ggcggcagaa gcaaaaggtg tgagcgacaa
    13561 catgcaattt atacattgcg ccgctcagga tgttgcttcg catttggaaa cgcccgttga
    13621 tctgatattg ttccatgcgg tgctcgagtg ggtggccgat ccccgcagcg tattacagac
    13681 cctgtggtca gtgttgcgtc caggcggtgt gttgtcgtta atgttctaca atgcgcacgg
    13741 cttgctgatg cataacatgg tcgccgggaa ttttgattac gtgcaggcgg gaatgccgaa
    13801 aaagaaaaaa cggacgcttt cgccagatta tccacgcgac ccggcgcagg tttatctgtg
    13861 gctggaagaa gctggttggc aaattatggg taagacaggc gttcgcgtgt ttcatgatta
    13921 tctgcgcgag aagcaccagc agcgcgactg ctatgaagca ttacttgaat tagaaacgcg
    13981 ttattgccgt caggaaccgt atattaccct ggggcgttat attcatgtca ccgcgcgcaa
    14041 accgcagagc aaggataaag tatgagtgaa ttttcccaga cagtccccga actggttgcc
    14101 tgggccagaa aaaatgactt ctccatctcg ctgccggtag accgactctc ttttctgctg
    14161 gcggttgcca cgctgaacgg cgagcgtctg gatggtgaga tgagtgaagg cgagctggtg
    14221 gatgcattcc gccatgtgag tgatgcgttt gagcaaacca gcgaaaccat cggcgtgcgc
    14281 gccaacaacg cgatcaacga catggtgcgt caacgtctgc tgaaccgctt taccagcgag
    14341 caggcggaag ggaacgcaat ttaccgtctg acgccgctcg gcatcggcat tactgactat
    14401 tacatccgtc agcgcgagtt ttctacgctg cgcctttcta tgcagttgtc gattgtggcg
    14461 ggtgagctca aacgcgcagc agatgccgcc gaagagggcg gtgatgaatt tcactggcac
    14521 cgtaatgtct atgcgccgct gaaatattcg gtagcagaaa tttttgacag tatcgacctg
    14581 acgcaacgtc tgatggacga acagcagcag caggtgaagg acgatatcgc ccagttgctg
    14641 aacaaagact ggcgggcggc gatctccagc tgtgaattgt tgctttcgga aacttccgga
    14701 acgctgcgtg aattgcagga tacgctggaa gcggcaggcg acaaattgca ggctaatctg
    14761 ttgcgcattc aggatgcgac gatgacccat gacgatctgc attttgtcga tcgtctggta
    14821 ttcgatctgc agagcaaact cgaccgtatt atcagctggg gccagcaatc catagacttg
    14881 tggattggct acgaccgcca cgtacacaaa tttatccgta ccgcaatcga tatggataaa
    14941 aaccgcgtct ttgctcagcg gttacgtcag tcggtacaaa cctattttga tgagccgtgg
    15001 gcgctaactt atgccaatgc cgatcgtctg ttggatatgc gtgacgaaga gatggctctg
    15061 cgcgatgaag aagtgacagg agaacttcct gaggatctgg agtacgaaga gtttaacgag
    15121 atccgcgaac agctggcggc gatcatcgaa gaacaactcg ccgtgtacaa aaccagacaa
    15181 gtgccgctgg atcttggtct ggtggtacgc gaatatctgt cacagtatcc gcgtgcacgt
    15241 cactttgacg ttgcgcgcat tgttattgat caggcggtac gtcttggcgt agcgcaagca
    15301 gatttcaccg gactgccagc gaaatggcag ccgattaatg attacggagc caaggtacag
    15361 gcgcatgtca tcgacaaata ttgaacaagt gatgccggtt aagctggcgc aggcgctggc
    15421 gaatccgtta tttccggcgc tggatagcgc cttacgttca ggccgccata ttggcctcga
    15481 cgaactggat aatcatgcat tcctgatgga ttttcaggaa tatctggaag agttttacgc
    15541 gcgttataac gttgagctta ttcgtgcacc ggaagggttc ttctatttac gcccacgttc
    15601 caccacgctg atccctcgtt ccgtcttgtc ggaactggat atgatggtcg ggaaaatcct
    15661 ctgttatctc tatctcagcc cggaacggct ggcgaatgag gggattttta ctcaacagga
    15721 actgtacgac gaactgctca ccctggccga tgaagcgaaa ctgctgaaac tggtgaacaa
    15781 ccgttcaacc ggttcagacg ttgaccggca gaagttgcag gagaaagtac gttcttcgct
    15841 caaccgtctg cgtcgtttgg gcatggtgtg gtttatgggc cacgacagca gcaagttccg
    15901 cattaccgaa tcggtgttcc gcttcggagc cgatgtgcgt gctggcgacg atccccgtga
    15961 agcacagcgt cgcctgattc gtgatggcga agcaatgccg attgaaaatc atctgcaact
    16021 caacgatgaa accgaagaga gtcagccaga tagcggagag gaagaataat gattgaacgc
    16081 ggtaaatttc gctcactgac gctgattaac tggaacggct tttttgcccg aacttttgac
    16141 cttgacgagc tggtcacgac gctttctggc ggtaacgggg cgggtaaatc caccaccatg
    16201 gcggcgttcg ttacggcgct gatccccgac ctgaccctgc tgcatttccg taacactacg
    16261 gaagccgggg ccaccagcgg ttcgcgtgat aaaggtctgc acggtaagct gaaagcgggt
    16321 gtctgttatt cgatgctcga caccattaac tcgcgccacc agcgcgtggt ggtcggtgtg
    16381 cgtctgcaac aggttgccgg acgcgatcgt aaagtggata tcaaaccatt tgccattcag
    16441 ggcctgccga tgtcggtgct gccgacgcag ttggtgaccg aaaccctgaa cgaacgccag
    16501 gcgcgcgtgc tgccgcttaa cgagctgaaa gacaagctcg aggcgatgga aggcgtgcag
    16561 tttaaacagt tcaactccat taccgattac cactcgctga tgttcgatct gggcatcatc
    16621 gcgcgtcgtc tgcgctccgc atctgaccgt agcaaattct atcgtctgat cgaagcctcg
    16681 ctgtatggcg ggatctccag cgccattact cgttctctgc gcgactacct gttgccggaa
    16741 aacagcgggg tgcgtaaagc gttccaggac atggaagcgg cactgcgtga aaatcgcatg
    16801 acgctggaag cgattcgtgt cacccagtcg gaccgtgacc tgtttaagca tctgatcagc
    16861 gaagccacca actacgtggc ggcggactac atgcgtcacg ccaacgagcg ccgtgtccat
    16921 ctcgacaaag ccctggaatt tcgtcgcgag ctacatactt cgcgtcagca attggcggct
    16981 gagcagtaca aacacgtcga tatggcgcgt gagctggcag agcacaacgg tgccgaaggc
    17041 gatctggaag cggattatca ggcggccagc gatcacctga acctggtgca aaccgcactg
    17101 cgtcagcagg agaagatcga acgttacgaa gcggatctcg atgagctgca gatccgtctg
    17161 gaagagcaaa acgaagtggt ggcagaagcc atcgaacgcc aggaagagaa cgaggctcgt
    17221 gcggaagctg ccgaactgga agtggacgag ctgaaaagcc agcttgctga ctatcagcag
    17281 gcgctggacg tccagcaaac gcgcgcgatc cagtataacc aggcgattgc tgcgcttaat
    17341 cgtgccaaag aactgtgcca tctgccggac ttaaccgccg acagcgccgc cgaatggctg
    17401 gaaaccttcc aggcgaaaga gctggaagcg actgaaaaaa tgctctctct tgagcagaaa
    17461 atgagcatgg cgcaaaccgc gcacagccag tttgagcagg cttatcagct ggtggtggca
    17521 attaatggcc cgctggcgcg taacgaggcg tgggatgtcg ctcgcgaact attgcgcgaa
    17581 ggggtcgatc agcgtcacct ggcagagcag gttcaaccgt tgcggatgcg cttaagcgaa
    17641 ctggaacagc gtctgcgcga gcagcaagaa gccgaacgcc tgctggcaga tttctgcaaa
    17701 cgtcagggca agaattttga tattgatgaa ctggaagctc tgcatcagga gctggaagca
    17761 cgcattgcct ctctttccga tagcgtgtct aacgcccgcg aagagcgcat ggcactgcgc
    17821 caggaacagg aacagctgca gtctcgcatt cagagtttga tgcagcgtgc gccggtttgg
    17881 ctggcagcgc aaaacagtct caaccagttg agcgaacagt gcggcgaaga gttcacctcc
    17941 agccaggacg tcaccgaata tctgcaacag ttgcttgagc gtgagcgcga agcgattgtt
    18001 gaacgtgacg aagttggcgc acgcaaaaac gccgtcgatg aagagatcga acgtttaagc
    18061 cagccaggcg gctctgaaga tcagcgtctg aacgcgctgg cggagcgttt tggtggtgtg
    18121 ctgctgtcag aaatttatga tgacgttagc ctggaagatg cgccgtactt ctcagcgctg
    18181 tatggcccgt cacgccacgc catcgtggtg ccagatctgt cacaggtaac cgagcacctg
    18241 gaaggcttga ccgattgccc ggaagatctc tatctgatcg aaggggatcc gcagtcattc
    18301 gatgacagcg tgttcagcgt tgatgagctg gaaaaagctg tagtggtgaa aatcgccgat
    18361 cgtcagtggc gttattcacg tttcccggaa gtgccgctgt ttggtcgtgc tgcgcgtgaa
    18421 agccgtattg aaagcctcca tgccgagcgt gaagtgcttt ccgaacgctt cgccacgctc
    18481 tcctttgatg tacagaaaac ccagcgtctg catcaggcat tcagccgctt tataggtagt
    18541 catctggcgg tggcgtttga gtctgacccg gaagcagaaa tccgtcaact gaacagccgt
    18601 cgcgtcgaac tggagcgggc gttgagtaat catgaaaatg ataaccagca gcagcgtatt
    18661 cagtttgagc aggcgaaaga gggtgttacg gcgctgaacc gcattctgcc gcgtctcaac
    18721 ttgttggctg atgacagcct ggcggatcgc gtcgatgaaa tccgcgaacg tctggatgaa
    18781 gcccaggaag ccgcgcgttt tgttcagcag ttcggtaatc aactggcgaa actggaaccg
    18841 atcgtttcgg tattgcagag tgacccggaa cagttcgaac agttaaaaga agattacgcg
    18901 tactctcagc agatgcagcg cgatgcccgt cagcaggcgt ttgccctgac ggaagtggtg
    18961 cagcgtcgtg cgcactttag ctactctgac tcggcagaaa tgcttagcgg taacagcgat
    19021 ctcaacgaaa aactgcgtga acgtctggaa caggcggaag cggagcgtac ccgcgcgcgt
    19081 gaagcgttgc gcggacacgc agcgcagttg agtcagtaca accaggtgct ggcttcgctg
    19141 aaaagttctt acgacaccaa aaaagagcta ctcaatgatc tgcaacgtga attgcaggat
    19201 atcggcgtgc gagctgatag cggggcagaa gagcgggcgc gtattcgccg tgacgagttg
    19261 cacgcgcaac tgagcaataa ccgttcacgc cgcaatcaac tggaaaaagc gcttaccttc
    19321 tgcgaagcag agatggacaa cctgacccgc aaactgcgca agctggagcg ggattacttt
    19381 gagatgcgcg agcaggtagt gaccgcgaaa gcgggctggt gtgcagtcat gcgcatggtg
    19441 aaagataacg gcgttgagcg ccgcttacac cgtcgtgagc tggcttatct ctccgctgat
    19501 gatttgcgtt ccatgtcgga taaggcgtta ggtgcgctgc gtctggcagt ggcagataac
    19561 gaacatctgc gcgatgtgct gcgcatgtcg gaagatccaa aacgtccgga acgtaaaatt
    19621 cagttcttcg tggcggttta tcagcatctg cgtgaacgta ttcgtcagga tattattcgt
    19681 accgatgatc cggtggaagc tatcgaacag atggagatcg aacttagccg tctgaccgaa
    19741 gagttaacct cccgtgaaca gaaactggcg atcagttccc gcagcgtggc gaacattatt
    19801 cgaaaaacca ttcagcgcga gcagaaccgt atccgtatgc tcaaccaggg gttgcagaac
    19861 gtatcgtttg gtcaggtgaa cagcgtgcgc ctcaacgtga acgtgcgtga aacgcacgcc
    19921 atgctgctgg atgtgctctc tgaacagcat gagcagcatc aggatctgtt taacagcaac
    19981 cgtctgacct tctcggaagc gctggcgaaa ttgtatcagc gtcttaatcc gcagattgat
    20041 atggggcagc gcacgccgca gaccattggc gaagaactgc tggattaccg caactatctg
    20101 gaaatggaag ttgaggttaa ccgtggttct gatggctggt tgcgcgcaga gtctggtgca
    20161 ttgtcgaccg gtgaggcgat tggtaccggt atgtcgattc tggtgatggt ggtacaaagc
    20221 tgggaagatg aatctcgccg cctgcgcggt aaagatatct ctccttgccg cctgctgttc
    20281 ctcgatgaag cagcgcgact ggatgctcgt tctatcgcca cgctgtttga attgtgtgaa
    20341 cgtttgcaaa tgcagctcat catcgcagcg ccggaaaata tcagcccgga gaaaggcacc
    20401 acctataaac tggtgcgtaa agtcttccag aataccgaac acgttcacgt cgtcggcctg
    20461 cgtggctttg cgccgcaact ccctgaaacg cttccaggaa gtgacgaagc gccttctcag
    20521 gcgagttaaa attaaggcgg cagcaatgcc gccttttctt tttccgaaaa ctccgtttct
    20581 gcactaaaaa agtggcacat tacggcgcgc ttttctttaa acttctttac attaggttat
    20641 gtaaaaacgt atcggcgttt atatactgaa gataagcctg atgagtaaca ggcttgctcg
    20701 tcatactttc gtgagtattg gcgttgtaca ggcaagtcgt aaaataacag cctggctatt
    20761 cagagtatga taaaaacagg gggcaaggga tgttgcttaa tatgatgtgt ggtcgtcggc
    20821 tgtcggcaat cagtttgtgc ctggccgtaa cattcgctcc actgttcaat gcgcaggccg
    20881 atgagcctga agtaatccct ggcgacagcc cggtggctgt cagtgaacag ggcgaggcac
    20941 tgccgcaggc gcaagccacg gcaataatgg cggggatcca gccattgcct gaaggtgcgg
    21001 cagaaaaagc ccgcacgcaa atcgaatctc aattacccgc aggttacaag ccggtttatc
    21061 ttaaccagct tcaactgttg tatgccgcac gcgatatgca acccatgtgg gaaaaccgtg
    21121 atgctgttaa agccttccag caacagctgg ccgaggtggc gattgccggt ttccagccgc
    21181 agtttaataa atgggtagag ttactgaccg atcctggtgt taacggaatg gcacgcgacg
    21241 tcgtgctctc tgatgcgatg atgggctatc tccatttcat tgcaaatatt ccggtcaaag
    21301 gcactcgctg gctatatagc agtaaacctt atgcgcttgc aacgccgccg ctttcggtga
    21361 ttaaccaatg gcagctggcg ctggataaag gtcaattgcc gacgtttgtt gcaggactgg
    21421 caccgcagca tccgcaatat gcggcgatgc atgaatcgtt actggcctta ctcagtgaca
    21481 ccaaaccgtg gcctcaactg accggtaaag caacgttgcg cccagggcag tggagtaacg
    21541 acgtaccggc gttgcgcgaa atattgcaac gcacaggcat gttggacggg gggccaaaaa
    21601 ttactctccc tggcgatgac acgccaactg acgcagtagt cagcccatcc gctgttactg
    21661 ttgaaacagc agaaactaag ccgatggaca agcaaacgac gtctcgtagt aaacctgcgc
    21721 ctgccgttcg tgccgcctac gataatgaac tggtggaggc cgttaaacgt tttcaggcat
    21781 ggcaaggatt gggagcagat ggcgctattg gcccggcaac gcgtgactgg ttaaacgtaa
    21841 cgcccgccca gcgtgctggc gtgttggcac tcaacatcca gcgattgcgc ttgctaccaa
    21901 cagagctttc taccgggatc atggttaaca ttccagccta ttcgctggtc tactatcaga
    21961 acggcaatca ggtactggat tcgcgagtca ttgtcggtcg ccccgatcgc aaaacgccga
    22021 tgatgagcag tgcacttaac aacgtagtgg taaacccgcc gtggaacgta ccaccaactc
    22081 tggcacgcaa agatattctg ccgaaagtgc gcaacgatcc gggatacctc gaaagccatg
    22141 gctatacggt gatgcgcggc tggaacagca gagaagcgat tgacccatgg caggttgact
    22201 ggtctacaat cacggcctcg aatttaccgt ttcgcttcca gcaggctcca ggcccacgga
    22261 actcgctggg gcgctataaa ttcaatatgc cgagttcaga ggccatttat ttgcatgaca
    22321 cgccgaacca caatctgttc aagcgtgata cacgcgcatt gagctcaggc tgtgtacgag
    22381 tgaataaagc ttccgatctg gcgaatatgc tgttgcagga tgcaggatgg aatgacaaac
    22441 gtatttctga tgcgctgaag cagggcgata cgcgttacgt caatattcgg cagtcgattc
    22501 cggtgaatct ctactacctg acggcctttg ttggtgcaga tggtcgtacc cagtatcgta
    22561 cagatattta caattatgat ctgcctgcgc gatccagctc gcaaatcgta tcgaaagcgg
    22621 aacaattaat caggtaaatg aagcagttcg gggaaatcag ttgtcgtaat aattagcatg
    22681 atcgggggcg attctctgca gcccccgtca ctgctggggt tgagtcatct tgacgtcagc
    22741 tttactgacg gttaaggtgc ctcttgtgcg ccagaagtgc atataaacga taacattgac
    22801 ctgtagactt gattatcatg gacaaattcg acgctaatcg ccgcaaactg ctggcgcttg
    22861 gtggcgttgc actcggtgcc gccatcctgc cgacccctgc gtttgcaaca ctctctaccc
    22921 cacgcccgcg tattttgaca ctcaataatc ttcataccgg agagtcaatc aaagcggagt
    22981 ttttcgatgg cagaggctat attcaggaag aattggcaaa acttaaccat tttttccgcg
    23041 attatcgcgc gaacaaaata aagtccatcg acccaggatt attcgatcag ttgtatcgcc
    23101 tgcaagggtt gttaggcacc cgcaaaccgg tgcaactcat ttccggttat cgctccattg
    23161 ataccaacaa tgaactacgc gcccgcagcc gtggagtagc gaagaaaagc tatcacacga
    23221 aagggcaggc gatggatttc catattgaag gtatcgcgtt aagcaatatt cgcaaagccg
    23281 cgttatctat gcgcgcaggt ggtgtaggat attacccacg tagtaacttt gtgcatattg
    23341 ataccgggcc agcacggcac tggtagtaat tgcttaacga aacaggggca gtatgaacta
    23401 tcgtattatt ccggtcaccg cattctccca gaactgttca ttgatctggt gtgaacaaac
    23461 ccgtctggcc gcactggtcg atcctggcgg cgatgcggaa aaaatcaaac aggaagttga
    23521 cgccagcggc ctgacactga tgcagatcct gctgacgcat ggtcatctgg accacgttgg
    23581 cgcagcggcg gaactggcgc aacattacgg cgtgccggtt tttggcccgg aaaaagaaga
    23641 tgagttctgg ctgcaaggct tgcctgcgca aagtcgtatg tttggtctgg aagagtgcca
    23701 gccgctgacg ccagatcgtt ggctgaacga aggcgatacc atcagcatag ggaatgtggc
    23761 tttacaggtg ttacattgcc ctgggcatac gccgggtcat gtcgtgtttt ttgatgatcg
    23821 ggcaaagctg ctgatttctg gcgacgttat tttcaaaggc ggagtagggc gcagtgactt
    23881 cccgcgtggc gatcataatc aactgatttc ttcaatcaaa gataagttgc tgccactggg
    23941 ggatgacgtg acatttattc cgggtcacgg accattatcc acacttggtt atgagcgcct
    24001 gcataatcca ttcctgcaag acgaaatgcc cgtctggtaa aggcgcataa aaaagcccgc
    24061 tttttaagcg ggcttcattg tttttaacgc ttacagcact gccacaatcg cttcgcacag
    24121 cggagccatg ttatctggtg tcatcccggc tacgttcaca cgaccagaag caacagcata
    24181 cacgccaaac tcttcgcgca gacgcagcac ttgttctttt gtcagaccac tgaaggagaa
    24241 catgccgttc tgtttgatga taaagctgaa gtcgcggttt gcgccttttt cctgcagcgt
    24301 attgacgaac aactgacgca tacgctgaat acgctggcgc atatcagtca gctcttgttc
    24361 ccaaatcgca cgtaacgcat cgttgctcag gatggtggca acaacagaag cgccgtgtgc
    24421 tggtgggtta gagtagttag cgcgaatcgc cgctttcatt tggctgaatg cgcgatcaac
    24481 ggtttcactg tcagcagcaa ccagagtaca agcgccaaca cgctcgttgt acaggccaaa
    24541 gtttttagag taggaactgg caacaatcag ctctttatgc atagccgcga aagcgcgcag
    24601 tccttcagca tcttcttcca gaccacgggc aaacccctgg taagcgaagt caaacagcgg
    24661 taaccagcct ttctcaacgg aaagttgtgc cagtgtctgc cattgttcca gcgtagggtc
    24721 gataccggtt gggttatggc agcagccatg gaacagcact acgtcgccag cctgagcttc
    24781 gttcaggctg ttaatcagtg catcgaagtc aagggtgtga ttttccgcat cataataagc
    24841 gtattcacga acttccagac ctgcagagtt aaagacgctc ttatggttcg gccagcttgg
    24901 gttgctcacc cacacacgct taacgctggt attttttgcc aggaaatcgg cagccacgcg
    24961 tagtgcgcca gtgccacccg gagtctgtgc cgtgcgagca cgtttgtcat tgatcagggc
    25021 gctaccttta ccaaacagca gttcctgagt gcagcgacca aattcaggga tgccgtcaat
    25081 gccgaggtaa tttttggtgg tttcattttc gagcaaatac tgttcagcct ttttcacgct
    25141 ggtcagtacc ggggttttgc ccgtctcatc tttatagaca ccaatcccga ggttaatttt
    25201 gccgggacgt tcatcggcac gaaacagatc ggccaggccc agaatcgggt cggcaggagc
    25261 ggcggtaatg ttctcaaaca tgacgaggtt ccattatggt tacagaaggg aagtccgcta
    25321 tcagggtaac gggagattta caaaattcca actattactg atgaaaacgc aggctgtttt
    25381 tgcaagacgt gagattgctc tggaaggcat aaaaaaaaca ggaccaacgt cctgtttttt
    25441 cggcatttaa caaagaggtg tgctattaga actggtaaac gatacccaca gcaacggtgt
    25501 cgtcagaacc tacgcccagt ttgttgtcag aatcgatctg gttgatgatg tagtcaacat
    25561 aggtggacat gtttttgttg aagtagtagg ttgcgcccac ttcaaagtag ttcaccagat
    25621 caacatcacc gataccttct acgtctttcg ctttagattt ggtgtaagct atggacggac
    25681 gcagaccgaa atcgaactgg tattgcgcaa ctaacagaac gtcttgcgtt ttgttggcga
    25741 agccgccttc aattggcgta gcgttacggg tttcaccgta gttcgctgcc aggtagatgt
    25801 tgttcgcgtc atacttcaga ccagttgccc actgttcagc ttttttaccc tgaccgcgga
    25861 attcggcttc ctgggcgtcg gtacggtctg ctgcaccgta agcacctacg ataccaaagc
    25921 cttcgaattc gtagctgata gagccaccga cgccgtcgcc gttggaacgc tcaggaatac
    25981 cagcacgttc gtttttgccc aggtactgaa cggcaaagtt caggccgtca accagaccga
    26041 agaagttgga gttacgatag gtagcaacgc cgcctacacg accaacgaag aagtcatcgc
    26101 tgtatgccgt gtcaccgcca aactcaggca gcatatcggt gtaacccagt gcgtcgtaaa
    26161 ccacaccgta gttacgaccg tagtcgatag aacccgcatc agcgaatttc agacctgcga
    26221 atgccagacg agttttgtta cctttctggg catcagcgcc ttcagagttg ttaccctgga
    26281 agttatattc ccactgaccg tagccggtca gatcggaatt gatttgtgtt tcccctttaa
    26341 aaccaagacg ggcataagtt ttgtcgccgt ttccgccgta actgtttaca ccattgtctt
    26401 tagaaaaata atgcagaccg acagctttac cgtacagatc tactttgttg ccatctttgt
    26461 tatagatttc tgcagcgttt gcagtacctg ctactaacag agcagggacg atcactgcca
    26521 gaatattgcg cttcatcatt atttattacc ctcatggttt tttttatgac acctgccact
    26581 gccgtcaata agttctgtca ataaaaattt acggaactat tgatgagagt ttggtgtctt
    26641 tatgtgtctg caggcatctt tccattcaaa ctaacgtttc gctaccgtga aagtgctaca
    26701 aagataaaaa tttggtttca aaaagaaaaa atatgtaacc aaaagtaaaa t
//
LOCUS       DACBVI010000054        26432 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.054, whole genome shotgun sequence.
ACCESSION   DACBVI010000054 DACBVI010000000
VERSION     DACBVI010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.054"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            201..395
                     /locus_tag="IU325_10940"
     CDS             201..395
                     /locus_tag="IU325_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HAP5809538.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /locus_tag="IU325_10945"
     CDS             468..944
                     /locus_tag="IU325_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HAP5809539.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="IU325_10950"
     CDS             complement(941..1408)
                     /locus_tag="IU325_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP5809540.1"
                     /translation="MDATLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="IU325_10955"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="IU325_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP5809541.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="IU325_10960"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="IU325_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP5809542.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="IU325_10965"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="IU325_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP5809543.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="IU325_10970"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="IU325_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP5809544.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="IU325_10975"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="IU325_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP5809545.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="IU325_10980"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="IU325_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP5809546.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="IU325_10985"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="IU325_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP5809547.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="IU325_10990"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="IU325_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP5809548.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="IU325_10995"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="IU325_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP5809549.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="IU325_11000"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="IU325_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP5809550.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="IU325_11005"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="IU325_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP5809551.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="IU325_11010"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="IU325_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013975775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP5809552.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="IU325_11015"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="IU325_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP5809553.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="IU325_11020"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="IU325_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP5809554.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="IU325_11025"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="IU325_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP5809555.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="IU325_11030"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="IU325_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP5809556.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="IU325_11035"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="IU325_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP5809557.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="IU325_11040"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="IU325_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP5809558.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="IU325_11045"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="IU325_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP5809559.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="IU325_11050"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="IU325_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP5809560.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="IU325_11055"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="IU325_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP5809561.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="IU325_11060"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="IU325_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP5809562.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="IU325_11065"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="IU325_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP5809563.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="IU325_11070"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="IU325_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP5809564.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="IU325_11075"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="IU325_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP5809565.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="IU325_11080"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="IU325_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP5809566.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="IU325_11085"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="IU325_11085"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP5809567.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="IU325_11090"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="IU325_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP5809568.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15548..15916
                     /locus_tag="IU325_11095"
     CDS             15548..15916
                     /locus_tag="IU325_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HAP5809569.1"
                     /translation="MWLLDQWAERHITEAQSKGEFDNLPGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYKEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15927..16352
                     /gene="zntR"
                     /locus_tag="IU325_11100"
     CDS             15927..16352
                     /gene="zntR"
                     /locus_tag="IU325_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HAP5809570.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESA
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16408..16626
                     /gene="arfA"
                     /locus_tag="IU325_11105"
     CDS             16408..16626
                     /gene="arfA"
                     /locus_tag="IU325_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome alternative rescue factor ArfA"
                     /protein_id="HAP5809571.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVERNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="IU325_11110"
     CDS             complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="IU325_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP5809572.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="IU325_11115"
     CDS             complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="IU325_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HAP5809573.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="IU325_11120"
     CDS             complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="IU325_11120"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP5809574.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAVAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     VLPGFEEGWVTVQDASAQGCMAWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19896..20843)
                     /locus_tag="IU325_11125"
     CDS             complement(19896..20843)
                     /locus_tag="IU325_11125"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP5809575.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKALPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20858..21367)
                     /locus_tag="IU325_11130"
     CDS             complement(20858..21367)
                     /locus_tag="IU325_11130"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP5809576.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21497..22621
                     /gene="dprA"
                     /locus_tag="IU325_11135"
     CDS             21497..22621
                     /gene="dprA"
                     /locus_tag="IU325_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP5809577.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLSWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVAGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPLGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22593..23066
                     /gene="smg"
                     /locus_tag="IU325_11140"
     CDS             22593..23066
                     /gene="smg"
                     /locus_tag="IU325_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HAP5809578.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23095..23637
                     /locus_tag="IU325_11145"
     CDS             23095..23637
                     /locus_tag="IU325_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809579.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23642..24214
                     /gene="tsaC"
                     /locus_tag="IU325_11150"
     CDS             23642..24214
                     /gene="tsaC"
                     /locus_tag="IU325_11150"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HAP5809580.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGATGGRLNPSEIRDALTGELFRQG"
     gene            24219..25037
                     /gene="aroE"
                     /locus_tag="IU325_11155"
     CDS             24219..25037
                     /gene="aroE"
                     /locus_tag="IU325_11155"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP5809581.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGSKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELTK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            25034..25291
                     /locus_tag="IU325_11160"
     CDS             25034..25291
                     /locus_tag="IU325_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 family protein"
                     /protein_id="HAP5809582.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25267..25821)
                     /locus_tag="IU325_11165"
     CDS             complement(25267..25821)
                     /locus_tag="IU325_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP5809583.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            25850..26086
                     /locus_tag="IU325_11170"
     CDS             25850..26086
                     /locus_tag="IU325_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809584.1"
                     /translation="MTLGALLALCKSFSMQKSTVLCANAAKRVKKQQTEKKDQKSTCA
                     KNWDPYNAPPLRRQRETLHKMVVTTKRKNPEIQG"
     gene            26293..>26432
                     /locus_tag="IU325_11175"
     rRNA            26293..>26432
                     /locus_tag="IU325_11175"
                     /product="16S ribosomal RNA"
BASE COUNT         6796 a         6259 c         6832 g         6545 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagtttcgaa ttaaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaatttca ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgcgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc tatcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atccgacctg cgattagaac tggaaggcgc taaggatctc cgtgaggcta
      781 tcgcgtatgc cgacagcgtt catgactatg ttagccgtga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatt gactggctcg aaactgagct ggatctcatc ggtaaaatcg
      901 gtctacaaaa ttacctgcaa tcacaaataa aagttaaaga ttaacggcct gccagcaaac
      961 tatcccagcc tacaacgaaa cccgcagccg ccagaaatgg cccaaacggc agcgggtttt
     1021 ttaatgactc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggcaagaagg tccatgtatg ccaggcacca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgacg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaataa tcgcccccca taacgcatcg gctaaaccgt
     1261 caggattaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1381 aaatcagaaa gggaagggta gcgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagaa atcgctgatt tgttaacctg gttccggtga
     1501 aaaacgctat cttcacaact ttacaaattt cgtggaatac agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggta
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctattgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa accaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcacaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgtt gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctatcgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtact ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcctgg gtattgtaaa
     5221 accatggaac tctacctggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcgtt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggtaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattgttgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggta
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat tccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct ttctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttacaaa ggcaagggtg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactctg caacaccctg
    10081 ttaaaggtgt tcatacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgtactgg
    10201 ctaaagcgta tggttccacc aacccgatta acgtggttcg tgcaactatc gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcctgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaagtctcgt tctggcggtg gcgtacgtcg cggtttcgag gggggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctgtctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaactac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aatcaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gctacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccgggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 accatgttcc tgatgtggtt gggcgaacag atcactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtatttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cctggttggt gcgctgtaca ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtgcgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacctg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtc gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgatttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgcct ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca acgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agtatcccca cttatctaca atagctgtac tctttttgtt catcccctgg
    15541 agtatttatg tggttacttg accagtgggc agagcgtcat attactgagg cacaatccaa
    15601 aggtgagttt gataatcttc cgggaagtgg cgagccattg attctggatg atgattcaca
    15661 cgtgccaccg gaattacgtg cggggtatcg cttgctgaag aatgccggtt gcttaccgcc
    15721 agaacttgag cagcggaggg aagcaataca gcttctggat atcctcaaag gtatccgtca
    15781 tgatgatccg caatataaag aggttagtcg tcgattgtca ttactggaat tgaagctgcg
    15841 acaagctgga ttgagtaccg attttttacg cggcgattat gctgacaagt tgttggacaa
    15901 aatcaacgat aactagtgga gtatgtatgt atcgcattgg tgagctggca aaaatggcag
    15961 aagtaacacc cgacacgatt cgttattacg aaaagcagca gatgatggag catgaagtgc
    16021 gcaccgaagg tgggtttcgc ctgtataccg aaagcgctct ccagcgattg aaatttatcc
    16081 gccatgccag acaacttggt tttagtctgg agtcgatccg cgagttgctg tcgatccgta
    16141 tcgatcctga acaccatact tgtcaggagt caaaaggcat tgtgcaggaa agattgcagg
    16201 aagtcgaagc acggatagcc gagttgcaga gtatgcagcg ttccttgcaa cgccttaacg
    16261 atgcctgttg tgggaccgcc catagcagtg tttattgctc gattcttgaa gctcttgaac
    16321 aaggggcgag tggcgttaag agtggctgtt gattttttgc actgacagga ttacactcgc
    16381 gccgttaaat aaccaattgg agtttttatg agccgatatc agcatactaa agggcagata
    16441 aaggataatg cgatagaagc attactacat gatcccttat tccgacagcg cgtagagaga
    16501 aataagaaag ggaaaggcag ttacatgcga aaaggaaaac atggcaatcg ggggaactgg
    16561 gaggccagtg gcaagaaagt aaatcacttt tttaccactg gccttctgct ttcaggcgct
    16621 tgttaagagc ggttattctg ctctttcagc aaatcacgaa tttctgtcag taatacttct
    16681 tctttagttg gtgcaggcgc ggctgctggt tcttcttttt tccgattcag tttgttgatt
    16741 agcttaatcg ccataaagat ggcaaaggct acaatcagaa aatcaaagac gttttgaatg
    16801 aagacaccgt aatgcatcac aacagcaggg atatccccct gcgcatcgcg tagcgtgaca
    16861 gcaaactgtt tgaaatcgat cccgccaatt aataagccca gaggtggcat aatgatatcg
    16921 gcaaccagtg aagatacaat cttcccgaat gccgcaccga taatgacacc caccgccaaa
    16981 tccaccacgt tcccgcgcat cgcaaattcg cgaaattctt taataatgct catgttattc
    17041 tccctatgaa gccgacaatc ataagtctaa caaatgttaa gctattttcc tgccaggaat
    17101 ggatttaatt aatccttgat aatcaaaggg aaataataag gcgtcattag acgccttatt
    17161 aattacaaga agaaaggact tggctggaag agtctttcga cgtcggtaat aaacttttta
    17221 tctgtgagga acataattac gtgatcgcct tgctcaatgc gcagattgtc attggcaatc
    17281 atcacgtcgt ttccacgtac caccgctcca ataatcgttc ctggcggtag cttgatttca
    17341 tcaatgactc tgccgacaac gcgtgaggta ctttcatcac cgtgagcaac ggcttcaata
    17401 gcttctgcta cgccgcggcg caatgaggaa acaccaacaa tatctgcttt tcgcacatgg
    17461 ctaagcaacg cggaaatcgt tgcttgttgc ggtgaaatcg caatatcgat cacgctcccc
    17521 tgaaccagat ccacataagc gcgacgctgg atcaatacca tcaccttttt cgcacccata
    17581 cgtttggcga gcatggctga catgatattg gcctcgtcat cgttggtgac ggcaataaac
    17641 agatcaactt gatcgatatg ttcttcggcc agcagttctt gatccgacgc atcaccaaaa
    17701 aagacgatcg tattctgcaa cttttccgcc agttcggcag cgcgttgctg attacgttcg
    17761 atgagtttaa cgctgtaatc tttttccaga cgacgcgcca gccctgcacc gatattaccg
    17821 ccaccaacca gcatgatccg cttatacggt ttttccagtc gctgtaattc actcatcacc
    17881 gcgcggatat gctgtgaagc ggcaataaag aacacttcat caccagcttc aacaatggtc
    17941 gaaccttgcg gacgaatggg gcgatcgtgg cggaaaattg ctgccacacg agtatcgata
    18001 tgtggcatat gttcgcgcat ggtcgaaagt gcattaccaa tcagcgggcc gccataatag
    18061 gctttaacca cagcgaggct gactttaccc tcagcgaagt tcaccacctg caatgcgccg
    18121 ggatactcaa tcagtcggta aatattatcg ataaccaact gctctggtgc gatcagatga
    18181 tcaatcggta cagcatctga atgaaatagc ttatcggcat cgcgcacgta gtctggtgag
    18241 cggatacgag cgatgcgatt aggggtgttg aaaagtgagt aggctacctg gcaggcaacc
    18301 atattggttt catctgaact agttacagca accagcatat cggcgtcgtc ggcacctgcc
    18361 tcccgcaata cgcgtggatg agagccatgc ccctgcacga ctcgcaggtc aaatttatcc
    18421 tgcaaggtcc gcagacgctc accgttggta tcgacaacag taatatcgtt gttctcgcca
    18481 accaggtttt ccgccagtgt gccgccaacc tggccggcac ccagaatgat aattttcatc
    18541 agtcgcgacc cgttatctca tcactttttg attagcttag cgtaaaagaa gccgtcgccc
    18601 tcttcggcac caggcagatt ttgcttaccc ggttgttctg gtgttcctgt ttcgcaaagt
    18661 tcggcatcag cggtacgttg caaaaaggct ttaatctgca ggctattctc ttccggtaac
    18721 accgaacagg tggcatagac cagggttcca ccggatttta aatgcggcca aatggcgtcg
    18781 agaatttcag actgcaattg cgcgagttcc gggatatcgc gatcgcgacg taaccattta
    18841 atatctggat ggcgacgaat cacaccggtt gctgaacaag gcgcatctaa taaaatgcga
    18901 tcaaactgtt gctcgccaca ccattgagaa gggtaacggc catcaccttg tttcacggtc
    18961 gccttcatac caaggcgttt taaattgtcg taaacccgag agaggcgctg ttcgtcgata
    19021 tcaaccgcaa caacctgcgc ttcgggtgcc acctcaagga tatgcgttgt tttaccgccg
    19081 ggggcagcgc aaagatccag aatgtgttca ccgttttgtg gcgcaagcca ggccatgcaa
    19141 ccttgtgctg atgcatcctg aacggtaacc catccttctt caaaacctgg tagcacatga
    19201 acaggtgcag gtgtttccag ccgtacagca tcagggtaat ccgcatgcgg gaaacctttc
    19261 attcctgctt catccagcaa tgcaagccag ctgtcgcggg aatgatgcgt acgattaacg
    19321 cgcagccaca ttggcggacg ctggttattg gcttctacga tggattgcca ctgctctgga
    19381 tacgcttttt gcagacgttt cagcaaccag gaaggatgca gataacgtgc atcactggca
    19441 ttaaactcgg ctaataactc ttcttgctga cgttggaact ggcgtaatac gccgttaatc
    19501 aaccctttaa gttgcggacg cttaattgcg acagcacctt caaccgtttc agccagcgca
    19561 gcatgaggtg gaatgcgggt ataaagcagt tgatacaaac caaccataat caggtaatgc
    19621 acagtgcgct gtttgccggt catcggacgg gccattaact tattaatcag ccagtctaac
    19681 tgtgaaagcg tacgcagtac gccaaagcac aactcttgaa gaagtgcttt gtctttatcg
    19741 gaaacttttt gctggagcgg tggcagaatg ttgcttaatg attgcccttg ctcgacgact
    19801 tgttcaacgg cctgtgccgc catgctacgt aaattacgtt gttttttcat aagtataaaa
    19861 atgcccggca agaccgggct tagaagagtg gactatcaga ccagacggtt gcccggaaca
    19921 aaccattccc gacgagaatt caggaggtct tgcgcgctca tcgctttctt acccgcaggt
    19981 tgtaacgaga gcaggttcag gatgccatca ccagtcgcaa cctgaatgcc ttgtttattg
    20041 gcttcaagga tcgttcctgg tgcagcgttg gttgccgtat caatgaccga cgctttccag
    20101 actttaaccg gctgtccttc aatttccagc cagctcattg gccatggatt gaaagcgcga
    20161 atgcagcgtt caagctgcgc tgccgaaagc gaccagtcaa tacgcgcttc ttctttactc
    20221 aacttttcgg cgtaagtgac aagagtttcg tcctgaactt ctggtttcgc cgtgccgtct
    20281 gccagttgtt tcaatgtggt gataagccct tgtgggccaa gctctgccag cttgtcgtac
    20341 agagtaccac tggtatcttc tgcagtaatc gggcaggaga gcttatagag catgtcaccg
    20401 gtgtctaaac caacatccat ttgcataatg gtcacaccag tttctgcatc acctgcccat
    20461 agtgagcgtt ggattggtgc agcaccgcgc cagcgtggca gcagtgaacc atgaacgttg
    20521 atacagccaa gacgcggcat ctccagcact gctttaggca gaattaagcc ataggcgacg
    20581 acaaccataa catcagcctg tagatcggcg accagttgct ggttttcttg tggacgcagg
    20641 gaaaccggtt gaaaaacggg cagagctttt tcctcagcca gaactttaac cgggctgggc
    20701 atcagttttt taccgcgtcc tgccggtcgg tctggctggg tgaacacgcc aacgacgtta
    20761 tgaccagaag acaacagcgc gtcgagatga cgcgctgcaa agtcaggtgt acccgcaaaa
    20821 ataatacgta gtgattctga cacgttagtt cttatcctta agcccgggct ttcagacgat
    20881 ccagtttttc aactttctga cgaatacgtt gttgtttcag cggtgacaga taatccataa
    20941 acagtttgcc gaccaggtga tccatctcat gctgaataca gatggctaac agaccctctg
    21001 cttccagttc aaatggttta ccgtcgcggt ccagggcgcg aattttaact ttctctgcgc
    21061 gtggcactaa agcacgttgt tcagggatcg acaggcatcc ttcttcaatg cctgtttcgc
    21121 cgcttttttc taaaagctcc ggattgatta acaccagccg ttcgtcacgg ttttccgaaa
    21181 catcaataac aatgatacgt tgatggatat cgacctgggt tgccgccagg ccaatacctt
    21241 cttctgcgta catcgtctcg aacatatcat cgacgatacg ctgaatttct gcattcactt
    21301 cttctaccgg tttagcaact ttgcgaagcc gctcgtccgg aatatgtaac acttgcaaaa
    21361 ctgacataaa tctccagaga tgtgttcagg agttagaaag attatttctt ctattctaga
    21421 caaatccccc tctgattgac agcatcactg accaatcgca aagattgcta aggctgctta
    21481 tggcagggag ataaggatgg tcgatacaga aatttggctg cgtttaatga gcatcagcag
    21541 cttgtacggc gatgatatgg tccgtatagc tcactggctg gcaaaacagt cgcatattga
    21601 tgcggttgta ttgcagcaaa ctgggcttac attgcggcag gcacaacgct ttctttcatt
    21661 tccgcgaaag agtatcgaaa gctcactttc ttggttggag caacccaacc atcatttaat
    21721 tcctgcggac agcgaatttt atcctcctca acttcttgcg acgacagatt accccggcgc
    21781 actgtttgtt gcaggagaac tgcacgcgct gcattcattt cagcttgccg tagtggggag
    21841 tcgggcgcat tcatggtatg gcgagcgatg gggacgatta ttttgcgaaa ctctggcgac
    21901 gcgtggagtg acaattacga gtggactggc gcgtggaatc gatggtgtgg cgcataaagc
    21961 ggccttacag gtaaatggcg tcagcattgc tgtattaggg aatggactta ataccattca
    22021 tccgcgtcgc catgcccgac tggctaccag tttgcttgaa catggtgggg ctcttgtctc
    22081 ggaatttccc ctcgatgttc ctccccttgc ttacaatttc ccacgaagaa atcgcattat
    22141 cagtggtcta agtaaaggtg tactggtagt ggaagcggct ttgcgcagtg gttcgctggt
    22201 gacagcacgt tgtgcgcttg agcaggggcg tgaagttttt gccttgccgg gtccattagg
    22261 gaatccggga agcgaagggc ctcactggtt aataaaacaa ggtgcgattc ttgtgacgga
    22321 accggaagaa attctggaaa acttgcaatt tggattgcac tggttgccag acgcccctga
    22381 aaattcattt tattcaccag atcaggaaga cgtggcattg ccatttcctg agctcctggc
    22441 taacgtagga gatgaggtaa cacctgttga cgtcgtcgct gaacgtgccg gccaacctgt
    22501 gccagaggta gtaactcaac tactcgaact ggagttagca ggatggatcg cagctgtacc
    22561 cggcggctat gtccgattga ggagggcatg ccatgttcga cgtactaatg tatttgtttg
    22621 aaacctatat tcacacagaa gctgagttgc gtgtggatca agacaaactt gaacaggatc
    22681 ttaccgacgc aggatttgat cgagaagata tctacaatgc cctgctttgg ctggaaaagc
    22741 ttgcggatta tcaggaagga ctggcagaac cgatgcaact ggcctctgat ccactctcca
    22801 tgcgtattta tacccctgaa gagtgtgaaa gactggatgc cagctgtcgt ggttttttgc
    22861 ttttccttga gcaaattcag gtgcttaacc ttgaaacccg tgaaatggtg atagagcgag
    22921 tgctcgcgct ggataccgca gagttcgacc tggaagacct gaaatgggta atcctgatgg
    22981 tgttgttcaa tattccgggc tgcgaaaatg cgtaccagca aatggaagaa ttactctttg
    23041 aagtgaatga aggtatgctg cattaatcat ttctttaatt cagcataagt tgttatggcg
    23101 aaatcagcac tgttcacggt gcgtaataat gagtcctgcc caaagtgcgg ggctgaactg
    23161 gttattcgat ccgggaaaca cggtccgttt cttggatgct cacagtatcc ggcgtgtgac
    23221 tacgtccgtc ctctgaaatc ttcagcggat ggacatatcg tcaaagttct ggaggggcag
    23281 gtttgccctg catgtggcgc aaatctggta ttacgccagg gacgctttgg tatgtttatt
    23341 ggttgcagta actaccctga atgcgaacat accgaactta tcgataaacc ggacgaaaca
    23401 gcaattacat gcccccaatg ccggacgggc catctggtcc agcgccgctc ccgttatggc
    23461 aaaacgtttc actcctgtga tcgctacccg gagtgtcaat ttgccattaa cttcaaaccc
    23521 atagctggag aatgccctga gtgtcattat ccgctactca tcgaaaagaa aaccgcgcag
    23581 ggtgtaaaac acttttgtgc cagtaaacaa tgtggaaagc cggtttcggc ggaataataa
    23641 cgtgaataat aacctgcaag gagacgctat cgcagctgcg atagatgtcc tcaatgaaga
    23701 acgtgtcatc gcctatccaa cggaagccgt tttcggtgtc gggtgcgatc ctgatagcga
    23761 aacagcagtg atgcgactgt tggagttaaa acagcgtccg gttgataagg ggctgattct
    23821 aatcgccgca aattacgagc agcttaaacc ctatattgat gacaccatgc tgactgacgc
    23881 gcagcgtgaa actatatttt ctcgctggcc aggtcctgtc acctttgttt ttcccgcgcc
    23941 tgcgacaaca ccgcgctggt tgacgggccg ctttgattcg cttgctgtac gagtcaccga
    24001 ccatccgttg gtggttgctt tgtgccaggc ttatggtaaa ccgctggttt ctaccagtgc
    24061 caacttgagt ggattgccac cttgtcgaac agtagacgaa gttcgcgcac aatttggcgc
    24121 ggcgttcccg gttgtgcctg gtgcaacggg ggggcgttta aatccttcag aaatccgcga
    24181 tgccctgacg ggtgaactgt ttcgacaggg gtaacataat ggaaacctat gctgtttttg
    24241 gtaatccgat agcccacagc aaatcgccat tcattcatca gcaatttgct cagcaactga
    24301 atattgaaca tccctatggg cgcgtgttgg cacccatcaa tgatttcatc aacacactga
    24361 acgctttctt tcgtgctggt agtaaaggtg cgaatgtgac ggtacctttt aaagaagagg
    24421 cttttgccag agcggatgag cttactgaac gggcagcgtt ggctggtgct gttaataccc
    24481 tcaagcggtt agaagatgga cgcctgctgg gtgacaatac cgatggtata ggtttgttaa
    24541 gcgatctgga acgtctgtct tttatccgtc ctggtttacg tattctgctc attggcgctg
    24601 gtggagcatc tcgcggcgta ctactgccac tcctttccct ggactgtgcg gtgacaatca
    24661 ctaatcggac ggtatctcgc gcagaagagt tgactaaatt gtttgcgcac actggcagta
    24721 ttcaggcgtt gggtatggac gaactggaag gtcatgagtt tgatctcatt attaatgcaa
    24781 catccagtgg catcagtggt gatattccgg cgatcccgtc atcgctcatt catccaggca
    24841 tttattgcta tgacatgttc tatcagaaag gaaaaactcc ttttctggca tggtgtgagc
    24901 agcgaggctc aaagcgtaat gctgatggtt taggaatgct ggtggcacag gcggctcatg
    24961 ccttccttct ttggcatggc gttctgcctg atgtagaacc ggtcatcaaa ctattgcaac
    25021 aggagttgtc agcgtgaatc aggccatcca gtttccggat agggaagagt gggacgagaa
    25081 taaaagatgt gtatgttttc ccgctctcgt gaatggtatg caactgacat gtgcgatctc
    25141 tggcgagagt ctggcgtatc gctttactgg agatacgcca gaacagtggt tagcgagttt
    25201 tcgtcagcat cgctgggacc tggaagaaga agcggaaaac ttaattcagg aacaaagtga
    25261 agatgatcaa ggttgggtct ggttaccttg atccagatat tcgtccttcc atttcacgta
    25321 gttatttgcg gaatagcgta acccagcctt ctcttcatca cttaacgggc ggatctgttt
    25381 gacggggcta ccgagataca gatatccgct ctccagccgt ttattttgtg ggaccaggct
    25441 acccgcacca atcatcacat catcttctac tattgcgcca tcaagtaaaa ttgagcccat
    25501 cccaaccaaa actcgattgc caatggtgca gccgtggagc atcaccttgt gaccaacagt
    25561 gacatcttcg ccaatggtta atgggttgcc atctgggttg tacgaggatt tatgagtgac
    25621 atgcaacata ctgccatcct ggatattggt gcgtgctccg atctgtacat aatgtacatc
    25681 tccacgaatc acaacgagcg gccagatccc cacatcatca gccagacgaa cgtcaccaat
    25741 cacgacgctg ctatcgtcga tcattacgcg ctgaccgatt tgtggaaaaa gatcgcggta
    25801 tgggcgtaaa acatcagaca tactcacctc aacaataaat gatttcctaa tgactttggg
    25861 tgcattattg gccttgtgca agtcttttag tatgcaaaaa agcaccgttt tgtgtgcgaa
    25921 tgcagcaaaa agggtgaaaa aacaacaaac agaaaaaaaa gatcaaaaaa gtacttgtgc
    25981 aaaaaattgg gatccctata atgcgcctcc gttgagacga caacgtgaaa cacttcacaa
    26041 gatggtcgta acaacaaaga gaaaaaatcc tgaaattcag ggttgactct gaaagaggaa
    26101 agcgtaatat acgccacctc gcgacagtga gctgaaagct gcgtcgcaac tgctctttaa
    26161 caatttatca gacaatctgt gtgggcactc gaagatacgg attcttaacg tcgcaagacg
    26221 aaaaatgaat accaagtctc aagagtgaac acgtaattca ttacgaagtt taattctttg
    26281 agcatcaaac ttttaaattg aagagtttga tcatggctca gattgaacgc tggcggcagg
    26341 cctaacacat gcaagtcgaa cggtaacagg aagcagcttg ctgctttgct gacgagtggc
    26401 ggacgggtga gtaatgtctg ggaaactgcc tg
//
LOCUS       DACBVI010000055        26420 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.055, whole genome shotgun sequence.
ACCESSION   DACBVI010000055 DACBVI010000000
VERSION     DACBVI010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.055"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            101..643
                     /gene="ecpR"
                     /locus_tag="IU325_11180"
                     /gene_synonym="matA"
     CDS             101..643
                     /gene="ecpR"
                     /locus_tag="IU325_11180"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HAP5809585.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVP"
     gene            718..1305
                     /gene="ecpA"
                     /locus_tag="IU325_11185"
                     /gene_synonym="matB"
     CDS             718..1305
                     /gene="ecpA"
                     /locus_tag="IU325_11185"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HAP5809586.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1362..2030
                     /locus_tag="IU325_11190"
     CDS             1362..2030
                     /locus_tag="IU325_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809587.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIQNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYRFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2056..4581
                     /locus_tag="IU325_11195"
     CDS             2056..4581
                     /locus_tag="IU325_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809588.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGPFGSLGLRAGIQRYNNGDSSANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFDTGLENDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4571..6214
                     /gene="ecpD"
                     /locus_tag="IU325_11200"
     CDS             4571..6214
                     /gene="ecpD"
                     /locus_tag="IU325_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HAP5809589.1"
                     /translation="MRVNLLIAMIIVALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRSGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6183..6893
                     /locus_tag="IU325_11205"
     CDS             6183..6893
                     /locus_tag="IU325_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HAP5809590.1"
                     /translation="MFRRRGVTLTKALLTVVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNLTKRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGEVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7206..7535
                     /locus_tag="IU325_11210"
     CDS             7206..7535
                     /locus_tag="IU325_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HAP5809591.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(7784..8398)
                     /locus_tag="IU325_11215"
     CDS             complement(7784..8398)
                     /locus_tag="IU325_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HAP5809592.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            8804..9493
                     /gene="paoA"
                     /locus_tag="IU325_11220"
     CDS             8804..9493
                     /gene="paoA"
                     /locus_tag="IU325_11220"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit"
                     /protein_id="HAP5809593.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDFSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLDVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPENLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSPPERTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            9490..12237
                     /locus_tag="IU325_11225"
     CDS             9490..12237
                     /locus_tag="IU325_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAP5809594.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGVLAVITASNAGALGKGD
                     KNTARLLGGPTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVS
                     QPPEDTPDKNVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTS
                     NQMIDWYRTDLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVK
                     VMLPRPTIPNNTTHRPATLQHLRIGTDQSGKITAISHESWSGNLPGGTPETAVQQSEF
                     LYAGANRHTGLRLAMLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILN
                     DTQVDPADPTRRFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRN
                     NLLEKSGAWVHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSS
                     FPVSAGSGGQWGANTSTSGVYAACVKLREMIASAVGFDPEQSQFADGKITNGTRSATL
                     HEATAGGRLTAEESIEFGILSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGR
                     ILNPKTARSQVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVI
                     FLDDTDPISSPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDV
                     V"
     gene            12247..13200
                     /gene="paoD"
                     /locus_tag="IU325_11230"
     CDS             12247..13200
                     /gene="paoD"
                     /locus_tag="IU325_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HAP5809595.1"
                     /translation="MSYSLFDKDEHWHKPEQAFLTDDHRAILRFAVEALMSSKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGHYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETEQ
                     IRAPVGIFPKARDAHTLALSVLAEVAAVRLRQEDSCLPPSS"
     gene            complement(13379..14506)
                     /locus_tag="IU325_11235"
     CDS             complement(13379..14506)
                     /locus_tag="IU325_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5809596.1"
                     /translation="MTLCVFVLIASEFMPVSLLTPITRDLGVTEGLAGQGIAISGALA
                     VLTSLTLSTLAGKMNRKFLQLGMTVLMAVSGLIIALASSYLMYMVGRAMIGVAIGGFW
                     SMSAATAIRLVPQHQVTRALAIFNAGNALATVVAAPLGSYLGATVGWRGAFLCLVPIA
                     VVAFIWQCISLPSMDANKSHTSCGAVFRLFSRRMVVVGMIACGLFFMGQFALFTYVRP
                     FLESVTRVNSLGLSSILLIIGVAGFIGTLVVSAFLNRKFYPTLMAIPGLMAVIAIGLM
                     LTGHHVWTVSLLLGLWGMLATAAPTGWWTWIARTLPDNAEAGGGLMVAVIQLAIALGS
                     TAGGIVFDRLGWQSTFAMSSVLLLCAGVLTFVTARQKAGAL"
     gene            complement(14648..15706)
                     /locus_tag="IU325_11240"
     CDS             complement(14648..15706)
                     /locus_tag="IU325_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP5809597.1"
                     /translation="MSYADSTNPNAPVSMTDKWDKTFAESQKVDHRKVSFPNRYGITL
                     VGDLYLPKDRGDRKLAAIAVSGPFGAVKEQSSGLYAQTLAERGFVTLAFDPSYTGESG
                     GYPRNVASPDINTEDFSAAVDFLGLQKEVDRNRIGLLGICGWGGMALNDAAMDTRVKA
                     VATSVMYDMSRAMGHGVGDGKDRYSTADRRAVLQYLNEQRWKDAQSGTFAHGGHDINV
                     DSNGKVSAGERILPETLPANPHPVLKEFFDYYRMPRGFHERSVNSTGAWTATMPLSFM
                     NMPLLSYASEITIPTLIVTGEKAHSRYFAEDAYKAVGSKDKELVVVPGANHVDLYDNV
                     AGKIPFAKFEQFFQTKLK"
     gene            15971..16855
                     /locus_tag="IU325_11245"
     CDS             15971..16855
                     /locus_tag="IU325_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP5809598.1"
                     /translation="MAKRENYNELYLFMQVVREGSFTAAAQRLGLAQSGVSRSVRELE
                     ERLGVQLLVRTTRKLSLTQAGEQLYQKTASGFEMLDLGLATLAHYRETPSGTVRINAS
                     QHAIDKCLLPKLAVFKQRYPDIRLELINESRFVDIIEERFDAGVRLGPEVGQGMVAVR
                     ITPDMEMAIVGTPEHFRRYGFPQTPADLKAHPCIAYQFADGSVYQWELNQDDKKITHR
                     PEGQWALSDSYMEAEAARLGLGLAYVPVELVADDLEHGKLIRVLQRYSLRMEGLFLYY
                     PHRNVSPALRMVIDTLKI"
     gene            complement(17289..18209)
                     /locus_tag="IU325_11250"
     CDS             complement(17289..18209)
                     /locus_tag="IU325_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP5809599.1"
                     /translation="MDLRDSYARQVKLLMAALPYVAKESCFALKGGTAINLFVQDFPR
                     LSVDIDLAYKSFADRDTDLAAINDALKRITASLNGRPGITAIRQENKADEKRIIVNTA
                     DAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
                     PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERIATLYAQEFSG
                     MTQQETSLESLLSVTTLLPQALKSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAI
                     RWKQRNLAVLKDKNPAKYVAAVNKLERVLA"
     gene            complement(18196..18999)
                     /locus_tag="IU325_11255"
     CDS             complement(18196..18999)
                     /locus_tag="IU325_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809600.1"
                     /translation="MSIQRRQLPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYV
                     KNGWLRRIAHGVYCRTGREPDWVDAVYCLQSQWEKPVRVAGLTSLALQGHAHYTEQGK
                     PQVWLALPAYVKLPGWFSAFEQSATFVTLYTSGLTADVTPFTTELKIGDRQLAGSVPE
                     LAAYEIACGVPSLISFEHADALFQGLNLLSPRKLQALLRASHSVKTNRVMLYLARRNG
                     HPYFQYIDQSGIETGTGKRQIIPGGWLEPDYQITVPRAFKPEGVEDGSA"
     gene            19294..22503
                     /locus_tag="IU325_11260"
     CDS             19294..22503
                     /locus_tag="IU325_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017050819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809601.1"
                     /translation="MTYQAIFTGWDDLTIEDLLVAYRKAKADSFFENTFPVAIKFAEY
                     EQELLENLQKLLDLLQSEDGFSSNKKLIGKFRLLPKKLTTKKKHESQNGHVHFSNPKR
                     AADHLFNNFDLIPEFRIIGDFPVDSHIISALWINMVGHKFDASLDNCCYGARLKRIRN
                     DELFSNEQDNPFHISAVGSFSPYFQPYQKWRGDGLKAIRDELEKDRDIIAASLDLKSY
                     YHFIDPLAITSDDLYNTLNIKLTEDEKAFTAQLAVFLKHWSDGAAAFGKKIAYKTPVI
                     NGGLVIGLTASRIISNILLHHWDKLVIEKLSPIHYGRYVDDMFLVIRDTGTITNNHEF
                     MLLLQDRLGNDCVYLKNEQKQIWQIQQGEHFQGKTTIQLQSDKQKLFVLQGRAGIDLL
                     DSIEKEIYELSSEHRLMPSPDQLEHSTAAKVLSAAGSVGENADTLRRADGLTIRRLGW
                     SLQLRYVETLARDLPPSEWKEQREEFYQFAYNHILRADNLFAHFSYLPRLLGFAISMN
                     EWQHAEKIVLKAYESINLLASVITSGKEVNINGCKTRAVNDLWRCIKGTLSWLFVDAA
                     TRYYSPDRLFLDKRSKKEECLADTFFNHISQSLTNLKDLLDLRFDSADFYLKAPLVAR
                     ADLAKEPYKQIVKSQSAEKLVNQRDSKKEVKILKLMSDSSLIDIDVIKLFLKSTKNTR
                     LEKVAKGNRKNESYLPYIFPTRPLTPAEISELAPECVGLPSTSDKKPDERPSTIWAKY
                     TQALRGVWIKPTLLASEQDSDEATKKARPKKFIHIGTDRKHKVVVALTSIKTEEDDWA
                     KMACNKSNLSRSRYQRISELVNATLKLSPKPDYVLFPELSIPLRWVNSIADRLSSAGI
                     SLIAGTEYRHLDDNQLKSEAVLVLSDNRLGYPASVKIWQPKLEPAVGEDEALFSIYGK
                     SWDSTLNVKQRKPVYIHHGVNFGVMICSELQNSKARIRFQGALDALMVLSWNKDLDTF
                     ASLIESAALDIHAYTILVNNRKYGDSRVRSPAKEPFMRDIARVKGGDNDFVVAATLDI
                     DSLRAFQSRAKRWPKGGDKFKPLPEGFQLAKNRKKLPPK"
     gene            22664..22804
                     /locus_tag="IU325_11265"
     CDS             22664..22804
                     /locus_tag="IU325_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809602.1"
                     /translation="MTRQRLFLCCSHGYISMVGRAGASQDAPVSDKAGKANPARFHHP
                     RD"
     gene            22850..23320
                     /locus_tag="IU325_11270"
     CDS             22850..23320
                     /locus_tag="IU325_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809603.1"
                     /translation="MATILTTDSDLLDLLTVPFNATTDFTVLADYCARFAETLIETDD
                     PALRLALCGRLHAALALLQPTLHDPIPPHLTESLTVDTLPATPEHFISEPDSLCEYCL
                     ALLQLLLERPAEPKMEQTLTGLLCELVWYFTEGLKAPRWLRTADGVKFISEVAA"
     gene            23511..23669
                     /locus_tag="IU325_11275"
                     /pseudo
     CDS             23511..23669
                     /locus_tag="IU325_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(23684..23759)
                     /locus_tag="IU325_11280"
     tRNA            complement(23684..23759)
                     /locus_tag="IU325_11280"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23724..23726),aa:Thr,seq:cgt)
     gene            complement(23874..25127)
                     /gene="proA"
                     /locus_tag="IU325_11285"
     CDS             complement(23874..25127)
                     /gene="proA"
                     /locus_tag="IU325_11285"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP5809604.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESSVALHADVAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(25139..26242)
                     /gene="proB"
                     /locus_tag="IU325_11290"
     CDS             complement(25139..26242)
                     /gene="proB"
                     /locus_tag="IU325_11290"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP5809605.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
BASE COUNT         6694 a         6932 c         6736 g         6058 t
ORIGIN      
        1 gatttactaa agaaactgaa tgtacctgta aaaattacag gtttggaaag tagtgacatg
       61 gcaaaatgat tacagcaggg actatgaggt taaaaaccat atggaatgtc aaaaccgttc
      121 tgataaatac atctggtctc cccatgacgc ctacttctat aaaggactat ctgaactgat
      181 tgtggatatc gacagattaa tttatctatc gctggagaaa atcagaaaag atttcgtgtt
      241 tatcaatctc aatacggatt ctttaactga gtttataaac cgtgataatg agtggttatc
      301 cgcggtaaag gggaaacagg tcgtattgat tgcggccaga aagtcagaag ccttagcaaa
      361 ttattggtat tacaacagca atattagggg cgtggtatac gctggactga gtcgtgatat
      421 tagaaaagaa ctggcctatg tgattaatgg caggttcctg agaaaagata ttaagaaaga
      481 taaaatcact gaccgggaaa tggaaattat ccgcatgacg gctcagggaa tgctgcctaa
      541 atcgattgcc agaattgaaa attgtagtgt gaagacagtg tatacccatc ggcgtaatgc
      601 tgaggccaag ctgtactcaa aaatatataa gttggttccg taaactccag gcaagttagt
      661 tttaaaaaat gactcacagg gacatcacgt cctcaattca actcgggaag aaaagcaatg
      721 aaaaaaaagg ttctggcaat agctctggta acggtgttta ccggcatggg tgtggcgcag
      781 gctgctgacg taacagctca ggctgtagcg acctggtcag caacagccaa aaaagacacc
      841 accagtaagc tggttgtgac gccactcggt agcctggcgt tccagtatgc cgaaggcatt
      901 aaaggtttta actcacagaa aggtctattt gacgtagcta tcgagggtga ctcaacggct
      961 accgccttta aactgacctc acgtcttatc accaacacct taacccagtt ggatacctca
     1021 ggttccacac tgaatgtggg cgttgattat aacggcgcgg cagtcgaaaa aactggcgat
     1081 accgtgatga tcgataccgc caacggcgta ctgggcggca accttagccc actggctaac
     1141 ggttacaatg ccagcaatcg taccaccgca caggatggtt tcactttctc catcatcagc
     1201 ggcaccacca atggtaccac cgcagtaacc gattacagca ctctaccgga aggcatctgg
     1261 agcggcgacg ttagcgtaca gttcgacgct acctggacca gttaatctct ctgatgtacc
     1321 agcaggggta cccccctgct ttattcctgg acggaattgt tatgaaaaag caccttctgc
     1381 ctctcgctct gctgttttcc ggaatatctc cggcccaggc gctggatgtc ggcgatatat
     1441 catcgtttat gaacagtgac agcagcacgc tgagcaaaac gatccaaaac agtaccgaca
     1501 gtggccgcct catcaatatc cgtctcgaac ggctctcttc accgcttgac gacgggcagg
     1561 ttatcgcaat ggacaagccg gatgagttgc tactcactcc cgctagcttg ctgctacccg
     1621 cccaagccag cgaagtgatc cgcttcttct ataagggacc agcagatgaa aaagagcgct
     1681 actaccgcat tgtctggttc gatcaggccc tcagtgatgc gcagcgcgat aatgccaacc
     1741 gcagcgctgt agccactgct tccgcccgca tcggcaccat tctggtcgtc gccccccgcc
     1801 aggcgaacta ccgctttcag tacgccaacg gctccctgac gaatacagga aatgcgacgc
     1861 tgcggatcct cgcctacggc ccctgcctga aagccgccaa tggtaaggag tgtaaagaga
     1921 attactacct gatgccgggt aagtcgcgtc gttttacccg cgtggatacg gcggataaca
     1981 aaggacgggt tgcactttgg cagggtgata agttcattcc cgtgaaatag atagctgtgc
     2041 agatggataa cgacaatgcc tttacgacgg ttctccccag gactgaaagc ccagtttgcc
     2101 ttcggcatgg tctttttgtt cgttcagccc gatgccagcg ctgctgacat aagtgcgcag
     2161 caaatcggtg gggtgattat tccgcaggcc ttcagtcagg cgcttcagga cggcatgagc
     2221 gtcccgctct atattcatct tgccggtagc cagggtcgcc aggacgatca gcgaatcggc
     2281 agcgctttta tctggttgga tgatggacag ctacgcatcc ggaaaataca gctggaagag
     2341 agtgaagata acgccagtgt cagcgaacaa actcgacagc agctgatggc tctggccaac
     2401 gccccgttca atgaggccct taccatcccc ctgactgaca acgcgcagct ggatctcagc
     2461 ttgcgccaac tgctgctgca gctggtggtc aagcgcgaag cgctgggcac cgtactacgc
     2521 tcacgtagcg aagacatcgg gcagtccagt gttaacaccc tcagcagtaa tctgagctat
     2581 aacttcggcg tctataacaa ccagttgcgt aacggcggga gcaacacatc cagctatctg
     2641 tcgctgaata acgttactgc gctgcgcgaa catcacgtgg tgctcgacgg ctcactgtac
     2701 gggattggta gcggtcaaca ggacagtgaa ttatataaag cgatgtatga acgcgatttt
     2761 gctggtcacc gatttgccgg tggaatgctc gacacctgga acttgcagtc cttagggccg
     2821 atgaccgcca tttcagcagg gaagatttac ggcctttcct ggggaaacca ggccagctcc
     2881 accatcttcg acagcagcca gtcagccacg ccagtgatcg cctttttacc ggcggcgggc
     2941 gaagtacatc tcacccgtga tgggcgacta ctaagcgttc agaacttcac catgggcaat
     3001 catgaagtgg atacccgggg tctaccgtac ggtatttacg atgtggaagt tgaggtgatc
     3061 gttaacggtc gcgtgatcag caaacgcacc cagcgggtca ataagctgtt tagccggggg
     3121 cgcggcgtcg gtgcaccact ggcgtggcag atatggggcg gtagctttca tatggatcgc
     3181 tggtcggaaa acgggaaaaa gacgcgacca gctaaagaga gttggctggc aggtgcctca
     3241 acctccggct cactgagtac gcttagctgg gcggcaacgg gatatggata cgataatcag
     3301 gcggtgggtg aaacccgtct gacgctgccg cttggggggg cgatcaacgt taacctgcaa
     3361 aacatgctgg ccagtgacag ctcatggagc aacatcgcca gcatcagcgc cactctacct
     3421 ggaggcttca gttctctgtg ggttaaccag gaaaaaaccc gcattggcaa tcaattgcga
     3481 cgtagcgatg ccgacaaccg tgcaatcggc ggcacactca acctgaactc actgtggtcg
     3541 aagctgggta cgttcagcat cagctacaat gatgaccgcc gctacaacag ccattattac
     3601 acggcagatt actatcaaaa tgtctacagc ggtccctttg gttcgcttgg cctgcgggcc
     3661 ggtattcagc gctataacaa cggcgacagc agcgccaata cagggaaata tatcgctctc
     3721 gatctctcgc taccactggg caactggttt agcgcaggga tgacccatca aaacggctac
     3781 accatggcaa acctgtcagc acgcaaacag tttgatgaag gaaccattcg cactgttggt
     3841 gccaatctgt cacgagccat ctccggcgat accggtgatg acaaaaccct cagcggtggg
     3901 gcgtatgcac agttcgacgc tcgttacgcc agcggaacgc tgaacgtcaa tagcgcggcg
     3961 gacggctaca tcaataccaa tttgaccgcc aacggcagcg tcggctggca aggtaaaaac
     4021 atcgctgcca gcgggcggac cgatggcaat gctggggtga tattcgacac tggcctggag
     4081 aacgatggtc agatcagcgc caaaatcaac gggcggattt tcccgcttaa cggcaagcgt
     4141 aactatctcc cgctctctcc ctatggaaga tatgaggtgg agttacagaa cagcaaaaac
     4201 tcactcgaca gttacgatat cgtcagcggt cgcaaaagcc atctgactct ctatccaggc
     4261 aatgtcgctg tcattgagcc agaagtgaag cagatggtta ccgtctccgg tcgtatccgt
     4321 gcggaagacg gcacactgct ggctaacgca cggattaaca accatatcgg ccgaacccga
     4381 accgatgaaa acggcgagtt tgtcatggac gtggataaga aataccccac tatcgatttt
     4441 cgctacagtg gcaataaaac ctgcgaagtg gcactggaac tcaaccaggc gcgcggtgcc
     4501 gtctgggtcg gtgatgtggt ctgcagcggc ctctcatcgt gggcggcggt gacgcagaca
     4561 ggagaagaga atgagagtta acctactgat agcgatgata atcgttgcgc taatctggcc
     4621 tgcaaccgcg ctcagagcgg cagtgagcaa aaccacctgg gcggatgctc cggcacgcga
     4681 gtttgtgttt gtcgaaaaca actcagacga caactttttc gtcactcctg gcggggcgct
     4741 ggatccgcgc ctgaccggtg ccaaccgctg gaccggttta aaatacaatg gttcaggaac
     4801 catctatcag caaagcctcg gctacattga taacggttac aacaccggcc tttataccaa
     4861 ctggaagttt gatatgtggc tggaaaattc accagtttca tctcctttaa ctggcttgcg
     4921 ctgcatcaac tggtacgctg ggtgtaatat gaccaccagt cttatcctgc cgcaaaccac
     4981 cgacgccagt ggattttatg gcgcgacggt gaccagcggc ggcgcgaagt ggatgcacgg
     5041 catgttgtca gacgcgtttt accagtatct gcaacaaatg cccgtcggca gcagctttac
     5101 aatgaccatc aatgcctgcc agacctctgt gaactatgac gccagcagcg gcgcacgctg
     5161 taaggatcag gcctccggca actggtatgt tcgcaacgtc acccatacga aagcagcaaa
     5221 tctacggttg ataaataccc actcgctggc ggaggtgttt atcaacagcg acggagtacc
     5281 gactctgggc gaagggaacg ccgactgccg gacgcaaacc atcggcagcc gttcaggatt
     5341 aagttgtaag atggttaact ataccctgca aacaaacgga ctcagcaaca cctcaatcca
     5401 tatattcccg gcgatcgcca actcgtcgtt agcctcggcc gtcggggcgt acgatatgca
     5461 gttcagtctg aatggcagtt catggaaacc ggtgagcaat actgcctatt actacacctt
     5521 caacgagatg aagagcgcag actcgatcta tgttttcttc tcgagcaact tctttaagca
     5581 gatggtgaac ctcgggatca gcgatatcaa caccaaagat ctattcaact ttcgctttca
     5641 gaacaccaca tcaccggagt ctggctggta tgaattttct acctccaaca cgctgattat
     5701 caaaccccgt gatttcagca tcagtattat ctccgatgaa tatactcaga caccgtcgcg
     5761 ggagggatat gttggcagcg gcgagtcggc actcgatttc ggctatatcg taaccaccag
     5821 cggtaaaaca gctgccgacg aagtgctgat caaggtgacc ggacccgcgc aggtgattgg
     5881 cgggcgctcc tattgtgtct tcagctccga tgatggtaag gcgaaagtac cgttcccggc
     5941 gacgctttcc tttattaccc gcagcggagc tacaaaaacc tacgatgccg ggtgcgatga
     6001 tagctggcgg gatatgaccg atgcgctgtg gctgaccaca ccgtggactg atatctctgg
     6061 cgaagtgggg cagatggata agaccacagt caaattttcg attccaatgg ataacgccat
     6121 ttcgctgcgt acggtagatg ataacggctg gtttggcgaa gtcagcgctt caggagagat
     6181 tcatgttcag gcgacgtggc gtaacattaa ctaaggccct gctgacagtg gtctgtatgc
     6241 tggcggcacc tttgacacag gcgatttcgg tcggcaatct gactttttca ctgccgtccg
     6301 agactgactt tgtcagcaaa cgtgtagtga ataacaacaa aagcgcacgg atataccgta
     6361 ttgccatcag tgctattgat agcccgggca gcagtgaatt gcgcacccga ccggtggatg
     6421 gtgaactgct tttcgccccc cgccagctgg cgttgcaggc tggtgagagc gagtatttta
     6481 aattttacta tcatggtcca cgggataacc gcgagcgcta ctaccgggtc tcatttcgcg
     6541 aggtccccac tcgtaacctg acaaaacgca gccctaccgg cggtgaggtc agcacggagc
     6601 cggtggtggt gatggatacc attctggtag tacgaccacg tcaggttcag tttaaatggt
     6661 ccttcgacca ggtgacagga acggtaagta acactggcaa cacatggttt aagctactga
     6721 ttaagccagg atgtgattcg accgaagagg aaggcgatgc ctggtatcta cgtccgggag
     6781 aggtggttca tcagcctgag ttacgtcagc cggggaatca ttatctggtc tataacgaca
     6841 aattcattaa gattagcgat tcctgtccgg ctaagccccc ttcggcggac taagacttat
     6901 ccatcggtca agggaagaac tccacctcag agactcaaaa atcgtttaaa taatgtggaa
     6961 gcgatcgtca atgtcacctg taacgatggg ctttctcata tgtacaccat agtgttcagg
     7021 agacgcggaa aggtaccagg cagcctgcta acgagtggtt tattcacgaa ttagtacgta
     7081 aaatcggtaa cggctggaaa tcattcaata ctcacactat cgaaagttcc ccagccaacc
     7141 gcggtacgtt cttacatacg atgtaccgct gttctcttta cgatttatag ctgtactggt
     7201 gaattatgag caatctgaat ccatgcatga cgtgtggtgc ctgttgtgca tttttccgcg
     7261 tctcttttta ctgggccgaa gccgacgatg ctggcggtac cattcccgcc aggctcactg
     7321 aacaaatatc cccttttcac cgatgcatga gcggtaccaa tcagaaaaac ccccgatgta
     7381 ttgcccttgc aggaacaccg ggcaaaaatg cctgctgcac gatatataaa aatcgatcgt
     7441 ccacgtgcag agaattcgcc atgtctggtg agaacggagt cgtcaatgag gcttgcaatc
     7501 gtgcaagagc taaatacggg ctgacaccgt tataaatatg caataattaa ttgcactgcc
     7561 ccgccagcta taatcgcggg gcttcgtttt tcagggataa caaaacccga aacttcttcc
     7621 atctgccagc aacaatgcct cttcctctat agtgttctcc ggatctacag catcctgaag
     7681 tagcgcgatg tactcacggg ctatctgctt cagaaagtaa aaagcaatgt ttttaaccta
     7741 taaaaatggc gctgcatttg cgccattttt attattcaat gcattatctg tttgagccta
     7801 aagggatctc agggtctggc tcatgagtaa ttctgtttcg taaatctctg cgaataattt
     7861 caatggacca gaaccagact aaatgtccaa aaatttcaga aacattctca taccacggga
     7921 gatcaaacag aggtggcgtc agtcccatga gagggaatga aatcatatga acaaaaagtt
     7981 gggctaaagc acctgccagt aagccctgcc agagtttaat ttttggaaat acttcagcga
     8041 ccacacaata accgacagca aacactatcg aaaagataat gtgcgtaaca ccaacccagt
     8101 taaagacatg cccggcaaag gtataaacag ccgcattggg atctgtcagc cccaaccagt
     8161 ctcgaagaaa aatatacgga ggattgagaa aattacggga gcaatcaatt tgcccagcag
     8221 ccctgattaa tgattccggg ccacacgctg cattaaacat atccaccggg ctacgtggcg
     8281 gcaatggaac ttcagccccc cacttcacaa atgcggaaac aacgccagca atcagcccaa
     8341 tgaatgcagc aagaccataa cgtctgcggt tcggtggagt ttgttcaaat atattcatat
     8401 ctaccctgct tgtaccatta tgttatacac ctcttcagga gtattcataa aataagacaa
     8461 atgtaaagaa ctgtattgtt ttgtataatg caatggtgac taatcgccaa tgaatataag
     8521 caccatcatt tctccctatt tttatattaa aagtgacaac gatttacagg gtgatgctga
     8581 gctgacaatc acaggtttcc ttatcggttt ttgcatcgca caactaaagc aataaactag
     8641 ctcccgtcat cggcgaatat gaattaatta gccgggctca tagctgcatt cgcgttttaa
     8701 gaataatcct cctgctctca ccgactatgc ttaacgttta aaaaagcatc agcactctcg
     8761 caacgcactc ttattttccc ctttagaata ccggaggcct ggtatgagca accaaggcga
     8821 ataccccgaa gataatcggg ttgggaagca cgagccgcac gattttagtt tgacccgtcg
     8881 cgatctgatt aaagtcagcg ccgcaacggc ggcgaccgcc gtggtttatc ctcattctac
     8941 gcttgcggca agcgttccgg cagctacacc cgcgccagag ataatgcccc tgacacttaa
     9001 ggtgaacggc aaaaccgagc agttggacgt tgatacccga accacgctac tggacgcttt
     9061 gcgtgaaaat ctgcatctga tcggtaccaa gaaaggttgc gatcacggac agtgcggagc
     9121 ctgtaccgtg ctggtcaatg gtcgaaggct taatgcctgc ctgacgcttg cagtcatgca
     9181 tcagggggcc gagatcacca ccattgaagg cctgggttca ccagagaatc ttcaccccat
     9241 gcaggcggcc tttatcaagc atgatggctt ccagtgcggc tactgcacct ccgggcaaat
     9301 ttgctcatca gtagcggtgc taaaagagat tcaggacggc attcccagtc acgtcacggt
     9361 cgatttggtt tcccctccag aaagaacggc cgatgagatc cgtgaacgta tgagcggcaa
     9421 catctgtcgc tgtggtgcat acgctaacat ccttgccgcc attgaagacg ctgcggggga
     9481 gataaaatca tgaaggcgtt tacctatgaa cgtgtgaaca ccccggccga ggcggcactt
     9541 agcgctcagc gcgtacctgg cgcaaaattt atcgctggcg ggaccaatct gctggacctg
     9601 atgaagctgg aaattgaaac gccaacccac cttatcgatg tgaacgggct cgggctcgat
     9661 aagattgaag tgaccgacgc gggtgggctg cgcatcggcg cactggtacg gaacaccgac
     9721 ctggcggctc acgagcgcgt gcgtcgtgat tacgcggtac tctcccgcgc cctgctcgct
     9781 ggcgcgtctg gtcagttacg caatcaggca accaccgcag gtaatctgct ccagcgcacg
     9841 cgctgcccct atttttacga caccaatcag ccctgcaata agcgcctgcc cgggagcggc
     9901 tgcgcggcac ttgaaggctt tagccgtcag cacgcggtgg taggcgtaag cgaagcctgc
     9961 attgccaccc atccgagcga tatggcggtc gcaatgcggt tgctggatgc ggtggtggaa
    10021 accatcacgc cggagggaaa gactcgcagt atcacactgg ctgattttta tcaccctccg
    10081 ggaaaaacgc cgcacattga aaccgccctg cttcccggtg agcttatcgt tgcggtgacg
    10141 ttacctccgc cgctcggcgg aaaacatatc taccgcaagg tgcgcgatcg cgcctcctac
    10201 gcctttgccc tggtatcggt cgcggcgatt attcagcctg acggcagcgg gcgcgtcgcg
    10261 ctgggcgtac tggctgtcat taccgccagt aacgccgggg cactcggcaa aggcgacaaa
    10321 aacaccgcca ggctgttagg cggccccact attgaacact atcatcaggc cattgcgctg
    10381 gtagtggccg agaccttcga acaggcgcga gcggcggcct cgctggtgca ggcgcactat
    10441 cgccgtaata aaggagctta ctccctggcg gacgagaaac aggccgtcag tcagccgccg
    10501 gaagacacgc ccgataaaaa tgttggtgac tttgacgggg ctttcacctc cgctgcggtg
    10561 aagattgatg ctacctacac gaccccggac cagagccata tggcgatgga gccgcatgcc
    10621 tcgatggccg tctgggatgg aaataagctt accctctgga cctcaaatca gatgattgac
    10681 tggtaccgca ccgatctggc aaaaacgctg aaagtgcccg tggagaatgt gcgtattatc
    10741 tccccgtata tcggcggagg gtttggcggc aagctgttcc tgagaagcga tgcgctgctg
    10801 gcggccctcg ccgcccgtgc ggtgaaacgt ccggttaaag tgatgctccc ccgccccact
    10861 attcccaata acaccacgca ccgccccgcc acccttcagc acttgcgtat cggtactgac
    10921 cagagcggga aaatcaccgc tatctcacat gaaagctggt ccggaaacct gcccggcggc
    10981 acgccggaaa cggcggtaca gcaaagcgaa tttctttacg caggggcgaa tcgtcatacc
    11041 ggcctgcggc tcgccatgct tgatttgccg gaagggaacg ccatgcgtgc gcccggcgaa
    11101 gcccccggtc tgatggcgct cgaaatcgcg atcgatgaac tggcggaaaa agcgggcatc
    11161 gatcccgtcg agtttcgcat cctgaatgac actcaggttg accccgccga tccgacgcgc
    11221 cgcttctctc gccgtcagct tatcgagtgc ttgcgcaccg gagcggataa atttggctgg
    11281 aagcagcgca acgccacacc cggacaggta cgcgacgggg agtggctagt cggccacggt
    11341 gttgcggcgg gctttcgcaa taatctgctg gaaaaatcgg gtgcgtgggt tcacctggag
    11401 caaaacggca ccgttaccgt ggaaacggac atgaccgata ttggcactgg cagctacacc
    11461 attctggccc agacggcagc ggaaatgctt ggcgtaccgc tggagcaggt tgcggttcac
    11521 ctcggcgatt ccagtttccc ggtttctgcg gggtctggtg gacaatgggg cgcgaatacc
    11581 tccacctccg gcgtttacgc cgcctgtgtg aagcttcgcg aaatgattgc ctcggcagtc
    11641 gggtttgatc ctgagcagtc gcagtttgcc gacggcaaga ttaccaacgg tacccgaagc
    11701 gccacgctac atgaggccac cgcaggcggc agactgacag cggaagagag cattgaattc
    11761 ggaatactga gcaaggagta ccagcaatcg acctttgccg ggcattttgt ggaggtcggc
    11821 gtgcatagcg cgacgggaga agttcgggtc cggcgtatgc tcgccgtgtg cgcagcgggg
    11881 cgcattctga atccgaaaac tgcacgcagc caggtcattg gcgcgatgac tatgggcatg
    11941 ggcgcggcac tgatggagga gctggcggta gatgaccgtt tgggctactt cgttaatcac
    12001 gatatggcgg ggtatgaagt gccggttcat gcggatatcc caaaacagga ggtgattttc
    12061 ctggatgata ccgaccccat ctcctccccg atgaaggcca aaggtgtcgg tgagctgggc
    12121 ctgtgcggcg tgagcgcggc tatcgccaac gcggtgtata acgccaccgg tattcgggta
    12181 cgggattatc ccatcactct ggataagctg ctcgataagc tgccggatgt ggtttaagga
    12241 ggaacaatgt catactcgct ttttgacaaa gacgagcact ggcataagcc agagcaggcg
    12301 tttctcaccg atgaccaccg ggccattctg cgcttcgccg tagaggcgct aatgtccagt
    12361 aaaggagcgg tgctggtgac gctggtggaa atacgcggcg gcgcggcgcg cccgctcggg
    12421 gcgcagatgg tggtgcgcga agatggtcat tactgcggtt ttgtctctgg cggctgcgtg
    12481 gaggccgctg ccgcttttga agcgctggag atgatgggct caggccgcga tcgcgaaatt
    12541 cgctatggtg aaggttcgcc gtggtttgac atcgttctgc cctgcggcgg tgggatcacg
    12601 ctgacgctcc ataaactacg ctcggcacaa cctctgctcg ccgtgctgaa ccgcctggaa
    12661 cagagaaaac cggcgggtct gcgctacgat ccgcaagcac aatcgttggt gtgcctgccc
    12721 acgcaaaccc ggacgggctg gaatctcaat ggctttgagg tggggttcag accatgcgtc
    12781 aggctgatga tttacggacg ttctcttgag gcgcaggcaa ccgcgagtct tgcagcagcc
    12841 acaggctatg acagccatat cttcgatctt tttccggcct cagccagcgc tcagatcgat
    12901 accgatacgg cggtcatttt gctgtgccat gatctcaacc gggaactgcc agtgttgcag
    12961 gccgcgcgag aagcaaaacc cttttatctc ggcgcattgg gcagctatcg aacccacact
    13021 ttacgtctgc aaaagctcca cgagctggga tggtccaggg aggagacaga gcaaatccgg
    13081 gcacctgtcg ggatattccc caaagcccgg gatgcgcata ctctggccct ttccgtactg
    13141 gcagaagtcg ccgctgtacg tctccgtcag gaggattcat gcctgccccc atcgtcctga
    13201 tcctcgcggc ggggcgtggg gagcgcttcc tcacctccgg gggaaatacc cataaattaa
    13261 gagaaaaatc tgcagtagcc gtttaagaaa atcacctaaa tcgtttaaaa gagcgcccaa
    13321 cccatagtgg gcgcttatcg ccttgtgaaa cttattcagc aacgcgcgcc tcgccggact
    13381 aaagcgcacc ggccttctgg cgtgcggtaa caaaggtcag tacgccagcg caaaggagca
    13441 gcacgctact catagcaaac gtgctctgcc agccaaggcg gtcaaacaca atgcctcctg
    13501 ccgttgatcc aagggcgatg gcgagctgaa tcaccgcgac catgagcccg cctccagctt
    13561 cggcgttgtc aggcaacgta cgcgcaatcc atgtccacca tccggttggc gcggcggtgg
    13621 ccagcatgcc ccagagtcct aacagcagcg agaccgtcca gacgtgatgc ccggtaagca
    13681 tcagaccgat ggcaatgacg gccatcagtc cgggtatggc catcagcgtg gggtagaatt
    13741 ttctgttgag aaatgccgaa acaaccagcg tgccaataaa accggccacg ccgataatca
    13801 gcaaaatcga cgacaagcct aaggaattaa cccgtgtcac cgactccaga aacgggcgca
    13861 cataggtgaa tagcgcaaac tgccccatga aaaacaggcc acaagctatc atgccgacca
    13921 caaccatgcg gcggctaaat aagcggaata ccgcgccaca tgaagtgtgg cttttattcg
    13981 catccatgct tggcaggctg atgcactgcc agataaaggc gacaaccgcg atgggcacca
    14041 ggcacaagaa agcgcctcgc catccgaccg tagcccccag atagcttccc agcggtgcgg
    14101 ccacgaccgt cgccagcgca ttaccggcgt tgaaaattgc cagcgcacgc gtgacctgat
    14161 gctgcggcac cagacgaatc gccgttgccg ctgacatcga ccagaatccg ccaatagcca
    14221 caccaatcat cgcacgaccg accatataca tcagataact ggaagccagc gcaataataa
    14281 gccccgatac ggccatcaga accgtcatcc ccagctgcag gaacttacga ttcatctttc
    14341 cagctagcgt tgaaagcgtc aggctggtca gaaccgccag cgcgccggag atagcaatcc
    14401 cctgccctgc cagcccctcc gtcacgccta aatcgcgggt aatcggcgtg agcaggctga
    14461 cgggcataaa ttcagaagca atcagcacaa acacgcacag ggtcatggca aaaataccgc
    14521 cccagtatgc gcgctgatga tgaggtgata gtgtttggct tagtgtcgac atagtgaaat
    14581 ttcacaaaga tcaggataaa agccaccagc ctatggccgg tggctttgca tcaattcagg
    14641 agaggtttta tttcagtttg gtctggaaaa attgttcgaa cttcgcgaaa ggtatttttc
    14701 cggccacgtt atcgtacagg tccacgtgat ttgcccctgg taccaccacc aactctttgt
    14761 ctttactgcc aaccgccttg taagcatctt cagcaaaata gcgtgaatgt gctttctcac
    14821 cggtcacaat cagcgtcgga atggtgattt cactggcgta gctcaacagc ggcatattca
    14881 taaatgacag cggcatcgtt gccgtccacg ccccggtaga gttaacggaa cgttcgtgga
    14941 agccgcgcgg catgcgatag tagtcaaaga actctttcag taccggatga ggatttgccg
    15001 gcagcgtttc tggcagaatg cgctcgcccg cactgacctt gccgttgctg tcgacattaa
    15061 tatcatgacc accgtgagcg aacgttccgc tctgcgcatc cttccagcgc tgttcattca
    15121 gatattgcag aacagcacga cggtcggcgg tagaataacg gtctttgcca tcccccacac
    15181 catggcccat cgcccggctc atgtcataca tcacgctggt agctaccgct ttaacgcggg
    15241 tatccatcgc cgcgtcgttc agcgccatgc cgccccagcc acaaataccg agcagcccga
    15301 tacggttgcg atctacctct ttttgcagcc ctaagaaatc cactgccgcg ctgaaatctt
    15361 cagtgttgat atccggtgag gcgacgtttc gcggatagcc gccgctttcc ccggtgtaag
    15421 aaggatcgaa cgccagggta acaaaccctc gttccgccag cgtctgcgca tacaggccgc
    15481 tggattgctc tttcaccgcg ccaaatggcc cactgaccgc aatcgccgcc agcttgcgat
    15541 cgccacggtc tttaggcagg taaagatcgc ccactaaggt gatgccatat cggttcggga
    15601 acgagacttt acggtgatcg actttctggc tctcggcgaa cgttttatcc catttatctg
    15661 tcatggacac aggggcatta ggattggttg aatcagcata actcattgtc gtgactccac
    15721 ttaatgatgc gaaaagcagc atggtcggca tcgcggtttt tagcgtgcgg gtgaaggctt
    15781 tcataaaaat ccccataaaa aacaacagag agatacaggt ttccgacgga caacgctgcg
    15841 gtgctttact ttgatggggt cattatagtg agcagaatta gtgctgatta gggggcgatt
    15901 aatgctaaga ttaataccat attgttataa attaatgtgg agattgccgg catgaagggg
    15961 ggatgtttca atggcgaaaa gggagaacta caacgagctg tacctgttta tgcaggtggt
    16021 gcgggaaggc agctttaccg cagcggctca gcggctgggt cttgctcagt caggagtcag
    16081 ccgttctgtt cgcgagcttg aagaaaggct gggtgtccag ctgctagtgc gcaccacgcg
    16141 taaactgtcg ctgacacaag caggcgagca gctctaccag aagacggcat ctggatttga
    16201 aatgttagat cttgggcttg ccacgctggc gcattatcga gagacgccct ccggcacggt
    16261 gcgtatcaat gccagccagc acgcgattga taaatgcctg ctgccaaagc tggcggtatt
    16321 taaacagaga tatcctgata tccggcttga actgataaat gagagccggt tcgtcgatat
    16381 catcgaggaa agattcgatg caggtgtccg cctggggcct gaagtcggcc agggtatggt
    16441 cgcggtacgc atcacgcccg atatggagat ggcaattgtg gggactccgg aacattttcg
    16501 tcgctatggt tttccgcaaa ccccggcgga tttaaaggcg catccctgta tcgcctatca
    16561 gtttgccgac ggcagcgtat accagtggga actcaatcag gatgataaaa aaatcactca
    16621 tcgccctgaa gggcaatggg ccctatccga cagctatatg gaagcagaag ccgcccggct
    16681 gggcctcgga ttggcctatg ttcctgttga actggtcgcg gatgatttag aacacggtaa
    16741 gcttatcagg gtgttgcagc gttacagcct gcgaatggag ggattgtttc tctattaccc
    16801 tcatcgcaac gtctcccccg ccctgcggat ggtcattgat acattgaaaa tctgaacgtt
    16861 aacaacgacc gactcttcac tgtgcaaaat tgtgtcaggt agtccctctg cgttcaattc
    16921 cagggtctgc tttgagcgag aaagagggga ctatccagca atggagatca cccaaaagaa
    16981 aaaaccacct ggctccctcg acaaccttca tccgggcatt tagcctcaat caacattttt
    17041 atatctgtca ggttacttca ttatgaaaac tgatcatcat cgttgttttt gttgcaatag
    17101 tagaatacgc gtatttattc ctccatctct attaataacg gcaataaact gttcacttca
    17161 ctgatattta aaatatgtat ccttttcaag ctgttcagcg gcgtaagaaa aagtattaat
    17221 tatgaattcg atgatttaac catatctgtc tgattggtaa tcatccgtga ttaatgctgg
    17281 tctattgatt acgccagaac cctctctagc ttattcaccg ccgccacata cttcgccgga
    17341 tttttatctt taagcaccgc cagattgcgt tgcttccagc gaatagccgg aagatgctga
    17401 atttcaggat agcgataccg cgaccagtca gggctgtttt gcttaaaact gagcaagaac
    17461 tcgcgatcca gatccgtaag atgtgacttc aacgcctgcg gcagcaaagt cgttactgac
    17521 agaagcgatt ccagcgacgt ttcctgttgc gtcatccctg aaaattcctg cgcgtacaac
    17581 gtggcgatcc gctctgtatc ccagtttgga gccagcaact cggcgatggg gcggggatgg
    17641 cctgccagat aacaaagaaa accgtcaaag atctcccgca tcagtcccgg tttctccagc
    17701 atattcagca catcaaacag atcgcgcggg tgctgacgat caagcgcagc gcagatttta
    17761 ccgccataaa gatccgccag cgagacgaca ctcatggtgg tgaagccata ctccatttcc
    17821 acacgttcgc acacaggcat ttctgccgga ggcaacaaca gcccgcgcca gaccgggcta
    17881 acctcgatct taatctttgc atcagcggta ttcacaatga tgcgcttttc atccgcttta
    17941 ttttcctgcc gaatcgcggt gatccccggt ctgccgttca gcgatgcagt aatcctcttc
    18001 aaagcgtcgt tgatagccgc caaatcagta tcgcgatccg caaagctctt gtacgccaga
    18061 tcaatatcca ccgacagacg gggaaaatcc tgcacaaaca aattgattgc ggtgccgcct
    18121 ttcaacgcga aacaggactc cttcgccaca tacggcagag cggccatcaa cagtttgacc
    18181 tgccgggcat agctatcacg cagatccatc ttcgactcct tccggtttaa atgcgcgtgg
    18241 aacagtaatt tgataatccg gctccagcca gccgccgggt atgatttgcc gctttcccgt
    18301 acctgtttct ataccgcttt gatcaatata ctgaaaataa gggtgtccat tacgccgggc
    18361 aaggtaaagc atcacccggt tggttttaac cgaatggctg gcgcgaagca gtgcctgcaa
    18421 tttgcgtggg ctgagaagat tcaacccctg aaacagagca tcggcatgtt cgaacgagat
    18481 taacgaagga acgccgcagg cgatctcata cgcggccagt tccggaacac tgcctgctaa
    18541 ctggcggtcg ccaattttca gttccgtagt aaatggcgtt acatcagcgg taaggcctga
    18601 cgtatacagc gtgacaaacg ttgcggattg ttcaaaggca gaaaaccatc ccggcagttt
    18661 gacgtaagcc ggaagcgcga gccagacctg cggcttaccc tgttcggtat agtgcgcatg
    18721 accctgaagc gcaaggctgg ttagccccgc cacccgaaca ggcttttccc actgcgattg
    18781 caggcagtaa accgcatcca cccaatccgg ttcacgaccc gtccggcagt atacgccgtg
    18841 agcgatccgc ctgagccagc cattcttcac atagagataa gagagcttac gatctatgcc
    18901 gttgcgggta agccatgact ggagcagcac ctggccgggt agcgtgtgct gcatcagcca
    18961 gtttagatag tttggcaact gtcgcctctg aatagacatt agtacaaata tttaaaccag
    19021 tgtagattca gggatacgcc acagcaagaa atagtttact tattcctcat tttgtcgact
    19081 aaaaatcgac attaaacaaa aaattcaaac ttaatcactt tcgggaaaaa tgtgacaaat
    19141 atatgctcgg actggttgcg gggagcgtgt aacatggata caaatcaaaa ttattgccag
    19201 cctcactgat ggattactgg tgtcaagagc cccccttcgg gcatgaaacg gctggctaat
    19261 tctgtacaga ctgtaatcta aggacgataa cgcatgacat atcaggcaat tttcactggc
    19321 tgggatgatc tgacgattga agaccttctg gtcgcttacc ggaaagcaaa agccgatagc
    19381 ttctttgaga atacatttcc tgttgctatc aaatttgccg agtatgagca ggaattactt
    19441 gaaaacctgc aaaaactctt agatcttttg cagagcgaag atggattcag tagcaataag
    19501 aagttgattg gcaaatttcg tttgttaccg aaaaaattaa ccacaaagaa aaaacatgaa
    19561 tcccaaaatg gacacgtcca cttttctaat cctaaacgag cagccgacca tttatttaat
    19621 aattttgatc tgataccaga gtttcgtatt attggtgact tcccggttga tagtcacatt
    19681 atctctgcac tatggattaa catggtcggg cataaatttg atgccagctt agataactgt
    19741 tgctatggcg cgcggctaaa gcgtattcgt aatgatgaat tatttagcaa tgagcaggat
    19801 aatccattcc atatcagtgc cgtgggttct tttagcccct acttccagcc ctaccaaaaa
    19861 tggcgtggtg atggcttaaa agctatacgt gacgagttgg aaaaagatcg tgacattatc
    19921 gccgcctcac tggatttaaa aagttactat cattttattg atccactggc tataacctct
    19981 gatgatctct ataacacact aaacataaaa ctgactgagg atgaaaaagc gtttactgca
    20041 cagttagcag tattcttaaa gcactggtct gacggcgcag cggcatttgg aaagaaaata
    20101 gcgtacaaaa cacctgttat taatggtggt ctggtcattg gattaacagc cagtcggatc
    20161 atttcaaata tattgctaca ccattgggat aaattagtca ttgaaaaact atcaccaatt
    20221 cactacggtc gttatgtcga tgatatgttc cttgtaatac gcgatacagg gacaattact
    20281 aataatcacg aatttatgtt attgctgcaa gataggcttg gcaatgattg cgtttatttg
    20341 aaaaacgagc aaaaacaaat atggcaaata cagcagggcg agcatttcca gggtaagacc
    20401 accatccagt tacaatccga taagcaaaaa cttttcgtgc ttcaagggag ggctggaata
    20461 gacctgctcg acagtatcga aaaggagatc tacgagcttt ctagtgaaca ccgcttgatg
    20521 ccttcaccgg atcaactgga acactccacc gcagctaaag tcctttccgc tgccggtagt
    20581 gtaggtgaaa atgccgatac tctgcgccgt gcggatggat taaccattcg tcgtttgggc
    20641 tggtcactgc aattacgcta cgttgaaaca ctggcacgag atctgcctcc aagtgaatgg
    20701 aaagaacagc gggaagagtt ttatcagttt gcctacaacc atattcttag ggctgataat
    20761 ctatttgcac attttagtta tctgccaagg ctgcttggct ttgctatcag tatgaatgaa
    20821 tggcagcacg cggaaaaaat tgtacttaaa gcttacgaat ccatcaacct gttggcatcg
    20881 gtgattactt caggtaagga agtgaatata aatggttgca aaactcgagc agtaaatgat
    20941 ctttggcgct gtataaaagg cacattaagc tggctatttg ttgatgcagc gacacgatat
    21001 tacagtcctg acagattatt tcttgataaa cgttcaaaga aagaagagtg ccttgcggat
    21061 acatttttta atcatatttc acaaagtctg acgaatctaa aggatttact ggatcttcgc
    21121 tttgattcag cagattttta tttaaaagcg ccattggtag ctcgagctga tttagcaaag
    21181 gaaccttata aacagatcgt aaagagtcag tcggcagaaa aacttgttaa tcagcgtgat
    21241 agtaaaaaag aagttaaaat actgaaatta atgagcgact catcgcttat tgatattgac
    21301 gttattaagc tatttttgaa atcaaccaag aatacccgac tggaaaaagt ggctaaagga
    21361 aatcgtaaga acgaaagtta cctaccttac attttcccta cacgtccttt aacacccgct
    21421 gaaatatcag aactggcccc cgaatgtgtt ggattaccct ccacatccga caaaaaacca
    21481 gatgagagac cgtccaccat ttgggcaaaa tatactcaag cattacgcgg agtatggatc
    21541 aaaccgacgt tgctagcatc ggagcaggac tcagatgaag cgacaaaaaa agctcggcct
    21601 aagaaattca ttcatattgg cacagacagg aaacataaag ttgtcgttgc gctaaccagc
    21661 attaaaacag aggaggacga ctgggctaaa atggcctgca ataaatctaa cttgtcccgt
    21721 tcaaggtacc agcggatttc tgaactggtt aatgcaacat tgaaactatc tcctaaacct
    21781 gattatgttt tattccctga gctttcaatc ccgttacgct gggttaacag tattgctgat
    21841 cgtttgagtt cggcgggtat cagtctaatt gcgggaacag aataccgcca cttagacgat
    21901 aatcaactga agagtgaggc cgtacttgtc ctttcagata acagactcgg ctatccagcg
    21961 agtgtcaaaa tatggcaacc caagctggaa cccgccgtag gtgaagatga ggcattattt
    22021 tcaatttatg gtaagtcttg ggattcgaca cttaatgtta aacaacgtaa gccggtatat
    22081 attcatcacg gcgtcaattt tggcgttatg atttgctctg aactccagaa tagtaaagcg
    22141 aggatccgtt ttcagggcgc actcgatgca ttaatggtat tgagctggaa taaagatcta
    22201 gatacgtttg catcgttgat tgaatcagca gcgctggata ttcatgccta tactatttta
    22261 gtgaataacc gaaaatacgg cgatagtcgc gtacgttccc cggcaaaaga accctttatg
    22321 cgtgatattg ctcgtgtgaa gggcggtgat aatgactttg tggtcgctgc aacgctggat
    22381 atcgactcgt taagggcatt tcagagcagg gcaaaacgct ggcctaaagg cggcgataaa
    22441 ttcaaaccgt tacctgaagg attccagttg gcaaagaacc gcaaaaagct accgccaaaa
    22501 taagaaactg attttcgcta ttaataatca gggtattttt gcgtgagatg ttggtaaaca
    22561 tgatgtagcc cttgccactc atgaccaatc gcagtatctt tctcccgcgc ctgcaaaatc
    22621 aggcgtcggg attagcctcc tgaagaaatc ttatcggcga cacatgacgc gccagcgtct
    22681 ttttttgtgt tgttcgcacg gttacatctc aatggtgggc cgggcggggg cgtcgcaaga
    22741 cgcgccagtg tccgataagg ctggtaaggc taaccctgcc cggttccacc acccgcgaga
    22801 ttagcctctc cggtggtggc ttgaacaccc acttatcgga ggttgcccta tggctacaat
    22861 cctcaccact gacagcgatt tactcgatct attgaccgtc cccttcaatg ccaccacgga
    22921 tttcactgtc cttgctgact actgcgcccg ctttgccgaa accctgattg aaaccgacga
    22981 tccagcgcta cgactggcgc tctgcgggcg gcttcacgcg gctcttgcgc tactgcaacc
    23041 cacactacac gaccccatcc caccgcatct taccgaaagc ctgaccgtgg atacccttcc
    23101 cgccacacca gagcacttta tttccgaacc tgattccttg tgcgaatact gcctcgcact
    23161 actccagctt ctgctggaac gtccggcgga accaaaaatg gagcaaaccc tgaccgggct
    23221 gctgtgtgag ctggtctggt atttcacgga aggactcaaa gccccacgct ggctgcgtac
    23281 cgccgacggc gtaaaattta tcagcgaggt ggccgcatga tgcaacagga ctggcattcc
    23341 gccgacatta ttgcggtgct gaaaaagcgg ggaacgcctc tggcggcaca gagaaaaact
    23401 cattgcggaa gcgctgtaaa tatcgccaga aatcatctgg ccttcaaggt actttgatag
    23461 caaaggcaag caggctggtt cagtccagtg tttctcttct tgcattttca gaaatgggag
    23521 gctgggagtc tatcgaaatg gttcgtcgat atgctcacct cgcgcctaat caccttaccg
    23581 aacacgcacg gcaaatagac tcagtcctga acccatcggt cccaaatttg tcccagtcaa
    23641 aaaataagga aggtactaat gatgtgtaac ttattgattt taatggtgcc gataagagga
    23701 gtcgaacctc cgaccttcgc attacgaatg cgctgctcta ccaactgagc tatatcggcc
    23761 ctgaaaggac atgttcacga acgtgaatca cggtggacaa ggttaaaact aaccgggcga
    23821 tgcgtcaatg gccttgtgaa tcaaatggct acttttgcat cacccggttt tatttacgca
    23881 cgaatggtgt aatcaccaat gccgatccac ttgtaagtgg tcagtgcttc cagccccatt
    23941 gggccacgcg cgtggagttt ttgtgtgctt accgccactt ccgcacccag accaaactgg
    24001 ccgccatcgg taaaacgcgt agaggcgtta acgtaaacag cggacgaatc cacttcgtta
    24061 acaaaacgct gggcgttgcg catatcgcgg gtcaggatcg catcggagtg ttgtgtgccg
    24121 tgttcacgaa tatgggcgat ggcatcgtca agatcgctga cgattttaac gttcaaatct
    24181 aatgacagaa actcatcgtc atactcttcg gctttaacag ccaccacctt cgcggggcct
    24241 gcctgcaact gcgccagcgc agctacatct gcgtgtaatg ccacgctgct ttccgccatt
    24301 tgcttgctta atgcgggcag gaagctatcg gcgatgtttt tatttaccag caacgtttct
    24361 accgtattac atgtgctcgg acgctgagtt tttgcgttga cgattacttt cagggcttca
    24421 gcgatctctg cactttcatc aacgtaaata tggcatacgc ctataccgcc tgtgatcacc
    24481 gggattgtcg actgttcgcg gcacagctta tgcaggccag cgcctccacg cgggatcagc
    24541 atgtcgatgt atttatccat acgcagcatt tcactgacca gcgcacggtc aggattatca
    24601 atcgcctgca cggcacccgc cggtaagccg caggatttca gggcgtcctg aatcaccgct
    24661 accgttgcag cgttagtgcg acaggtttct ttgccaccac gcaggatcac cgcgttaccg
    24721 gttttcaggc acagcgaagc gacatcaacc gtcacgttcg ggcgcgcttc ataaatcacg
    24781 ccaataaccc ccagcggtac gcgacgacgc tcaagacgca ggccgctgtc cagtacgccg
    24841 ccatcgatta cctgccccac cggatcggcg agattacaca cctgacgcac atcgtcggcg
    24901 atgcctttca gccgtgcggg cgtcagtgcc agacggtcaa gcatcgcttc gctaaggcca
    24961 ttggcacgcg cgtcagcaac atcctgggcg ttagcgttga ggatgatttc gctttgtgct
    25021 tccagttcat cggcgatttt ttccagcacg cgattttttt cgcggctgga gagttgcgct
    25081 aatttatacg aggcttgctt cgcggcaatg cccatttgtt ccagcatcag cctgctcctt
    25141 aacgggtaat catgtcatca cggtgaacgg caaccgggcc atattcatat cccagtattg
    25201 catcaatttc ttgcgagtgg tgtccggcaa tgcggcgtaa tgcatcgctg ttgtaacgac
    25261 tgacgccgtg agcgatatcg cgaccttcga ggttgcaaat gcggatgact tcaccacgcg
    25321 agaaattgcc agtcacgctt ttaatgcctt tcggcaacag ggaactgccg cgttcaagaa
    25381 tggcggcagt tgccccttca tctaccgtga tttcacccgc cggcggcgca ccgaaaatcc
    25441 agcgtttacg gttttcaagc ggagtcgcct gggcatggaa cagcgtaccg acggaaatgc
    25501 cttccatcac atcaccaata acgcccggct tgctgcccgc ggcaataatg gtgtcgatac
    25561 ccgcacggca agccacgtcc gcggcctgca atttggtact catgccgcca gttccgaggc
    25621 ctgaaacgct gtcaccggca atcgcgcgca gtgcgtcatc aatgccgtaa acatctttaa
    25681 tcagttctgc ctgcggattg ctgcgcgggt cagcggtata caaacctttt tgatcggtca
    25741 gcagcaacag tttatcggca cccgccagaa tcgccgccag cgcagaaagg ttatcgttat
    25801 cgccgacctt aatctctgcc gtagcgacag catcgttctc attgattacc ggaacgatat
    25861 tgttatcgag caacgcacgc agggtgtcgc gggcgttcag gaagcgttca cggtcttcca
    25921 tatcagcacg agtcagcagc atttgcccga cgtgaatgcc ataaatcgaa aacagctgtt
    25981 cccacagttg aatcagtcga ctctgcccta ccgccgccag cagttgtttc gaggcgatag
    26041 tcgctggcag ttccgggtaa cccaggtgct cacgtccggc ggcgatcgcg cccgacgtca
    26101 caataacaat ccgatgcccg gcggcatgta actgcgcgca ctggcgaaca agttcaacga
    26161 tatgggcacg gttcaggcgg cgcgatccgc ctgttagcac actggtgccg agttttacca
    26221 ccagcgtctg gctgtcactc atgattctct gccattcaat tttaggaaaa atgatatcaa
    26281 acgaacgttt tagcaggact gtcgtcggtt gccaaccatc tgcgagcaaa gcatggcgtt
    26341 ttgttgcgcg ggatcagcaa gcgtagcggc agttgtttac gcttttatta cagatttaat
    26401 aaattaccac attttaagaa
//
LOCUS       DACBVI010000056        25921 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.056, whole genome shotgun sequence.
ACCESSION   DACBVI010000056 DACBVI010000000
VERSION     DACBVI010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.056"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(245..400)
                     /locus_tag="IU325_11295"
     CDS             complement(245..400)
                     /locus_tag="IU325_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002795307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809606.1"
                     /translation="MAALVVTWFNPVKEAFYMHLPAAGKTKKVALVDCMRKLLTILNA
                     MLRKNEE"
     gene            complement(626..701)
                     /locus_tag="IU325_11300"
     tRNA            complement(626..701)
                     /locus_tag="IU325_11300"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(666..668),aa:Phe,seq:gaa)
     gene            complement(807..1514)
                     /locus_tag="IU325_11305"
     CDS             complement(807..1514)
                     /locus_tag="IU325_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 family protein"
                     /protein_id="HAP5809607.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTTWFA"
     gene            1906..4041
                     /gene="speC"
                     /locus_tag="IU325_11310"
     CDS             1906..4041
                     /gene="speC"
                     /locus_tag="IU325_11310"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HAP5809608.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILTLLKRTGFHLPVFLYSEHVVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRTDMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVVDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVEIGIEARKAILAHCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4087..5343)
                     /locus_tag="IU325_11315"
     CDS             complement(4087..5343)
                     /locus_tag="IU325_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5809609.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(5545..6624)
                     /gene="mltC"
                     /locus_tag="IU325_11320"
     CDS             complement(5545..6624)
                     /gene="mltC"
                     /locus_tag="IU325_11320"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HAP5809610.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPGSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSITINMVPNHLDKRAHKYLGMVRQASRKYGIDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(6689..6964)
                     /locus_tag="IU325_11325"
     CDS             complement(6689..6964)
                     /locus_tag="IU325_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HAP5809611.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6992..8044)
                     /gene="mutY"
                     /locus_tag="IU325_11330"
     CDS             complement(6992..8044)
                     /gene="mutY"
                     /locus_tag="IU325_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP5809612.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHSG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVDRFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8205..8924
                     /gene="trmB"
                     /locus_tag="IU325_11335"
     CDS             8205..8924
                     /gene="trmB"
                     /locus_tag="IU325_11335"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP5809613.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPVLFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8924..9250
                     /locus_tag="IU325_11340"
     CDS             8924..9250
                     /locus_tag="IU325_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF469 family protein"
                     /protein_id="HAP5809614.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLGEVRTSEL
                     FDVWWD"
     gene            9434..10153
                     /locus_tag="IU325_11345"
     CDS             9434..10153
                     /locus_tag="IU325_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 family protein"
                     /protein_id="HAP5809615.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            10329..11375
                     /gene="ansB"
                     /locus_tag="IU325_11350"
     CDS             10329..11375
                     /gene="ansB"
                     /locus_tag="IU325_11350"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HAP5809616.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDISKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            11492..12499
                     /locus_tag="IU325_11355"
     CDS             11492..12499
                     /locus_tag="IU325_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HAP5809617.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     NQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVSIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(12555..13856)
                     /locus_tag="IU325_11360"
     CDS             complement(12555..13856)
                     /locus_tag="IU325_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAP5809618.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRDLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAVLLITLMMITYIPQISLFIPQLLGLM"
     gene            complement(13856..14359)
                     /locus_tag="IU325_11365"
     CDS             complement(13856..14359)
                     /locus_tag="IU325_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HAP5809619.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QPAQEAS"
     gene            complement(14404..15390)
                     /locus_tag="IU325_11370"
     CDS             complement(14404..15390)
                     /locus_tag="IU325_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HAP5809620.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSKGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQQQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
     gene            complement(15704..16840)
                     /gene="hemW"
                     /locus_tag="IU325_11375"
     CDS             complement(15704..16840)
                     /gene="hemW"
                     /locus_tag="IU325_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HAP5809621.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVQTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEANRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFSQYTGLS
                     EEVIRSQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(16833..17426)
                     /locus_tag="IU325_11380"
     CDS             complement(16833..17426)
                     /locus_tag="IU325_11380"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HAP5809622.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(17434..17724)
                     /locus_tag="IU325_11385"
     CDS             complement(17434..17724)
                     /locus_tag="IU325_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HAP5809623.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(17721..18287)
                     /gene="yggT"
                     /locus_tag="IU325_11390"
     CDS             complement(17721..18287)
                     /gene="yggT"
                     /locus_tag="IU325_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HAP5809624.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(18305..19009)
                     /gene="yggS"
                     /locus_tag="IU325_11395"
     CDS             complement(18305..19009)
                     /gene="yggS"
                     /locus_tag="IU325_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HAP5809625.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPSLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            19027..20007
                     /locus_tag="IU325_11400"
     CDS             19027..20007
                     /locus_tag="IU325_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HAP5809626.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLVNTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            complement(20122..20538)
                     /gene="ruvX"
                     /locus_tag="IU325_11405"
     CDS             complement(20122..20538)
                     /gene="ruvX"
                     /locus_tag="IU325_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP5809627.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(20538..21173)
                     /locus_tag="IU325_11410"
     CDS             complement(20538..21173)
                     /locus_tag="IU325_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708713.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HAP5809628.1"
                     /translation="MTAPFFPRILGAVAFLNQETEPLTMNLQHHFLIAMPALQDPIFR
                     RSVVYICEHNTNGAMGIIVNKPLENLKIEGILEKLKITPEPRDESIRLDKPVMLGGPL
                     AEDRGFILHTPPSNFASSIRISDNTVMTTSRDVLETLGTDKQPSDVLVALGYASWEKG
                     QLEQEILDNAWLTAPADLNILFKTPIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(21210..22160)
                     /gene="gshB"
                     /locus_tag="IU325_11415"
     CDS             complement(21210..22160)
                     /gene="gshB"
                     /locus_tag="IU325_11415"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HAP5809629.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(22173..22904)
                     /gene="rsmE"
                     /locus_tag="IU325_11420"
     CDS             complement(22173..22904)
                     /gene="rsmE"
                     /locus_tag="IU325_11420"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP5809630.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(22984..23691)
                     /gene="endA"
                     /locus_tag="IU325_11425"
     CDS             complement(22984..23691)
                     /gene="endA"
                     /locus_tag="IU325_11425"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HAP5809631.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(23786..24283)
                     /locus_tag="IU325_11430"
     CDS             complement(23786..24283)
                     /locus_tag="IU325_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HAP5809632.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MENVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(24360..25754)
                     /locus_tag="IU325_11435"
     CDS             complement(24360..25754)
                     /locus_tag="IU325_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP5809633.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
BASE COUNT         6584 a         6403 c         6658 g         6276 t
ORIGIN      
        1 ttgtattttt tctatatcta tttttcagga gattattatt aaatagaaaa ataacaatgg
       61 tgattatctc cggttgagct ggagtcacgt atgccagcga gtgctttctt ccgggccttc
      121 cttcacttca gagaaagagc ctatggaaaa gaggcaatca tcaggctctt tttgagtcat
      181 atgcgccgat aatcaactgt cttgaacgta aaaattatgg agcaacatga tgctacgatt
      241 catttcactc ttcgttcttt ctgagcattg cgttcaggat ggtcagcaat ttacgcatac
      301 agtcaaccag cgccactttt ttggtttttc cggctgctgg cagatgcatg taaaacgcct
      361 ctttcaccgg attaaaccag gttaccacaa gcgctgccat gtgcagggct gctcttgcct
      421 ccgtacgcgc actggcgtcg ttcggccagc agatatgtgt gggataacag ggatgttatg
      481 tgaactgaac tgtgttttgt tagcggggga tgtttattga tgattcggga gtgtttaaga
      541 cctgatggcg ttatgctccc aagtttattt tatataaaga tccaaaagca aaaacccgcc
      601 ttgtgggcgg gttcttaaga attagtggtg cccggactcg gaatcgaacc aaggacacgg
      661 ggattttcaa tcccctgctc taccgactga gctatccggg caacggggcg cattaaacct
      721 gattcgcctc gtctcgtcaa tcaaatttct tcaatttact gcagactgca caatctatca
      781 tcactttgcc gctaatgcac gcattgtcag gcaaaccagg tggtccaggc ggcggaaagc
      841 ggcattgcca gaaggagtgc tggaagcatg ttgaccaccg ggaacatttt aatgccacag
      901 atgcgtaatc cggttgccag cagcaataaa ccgcctacgg cgctgaagtc tgccatcatc
      961 gacggtgtgg tcagcggtaa tatcagcgcg gcagcccacg ccagcgttag ctggatgatc
     1021 agtaatggga tactaattac cgacaccgca atacccagcg agcaggcgaa gatcatcgcc
     1081 gtaaagaaat caagaaatga cttggcgatt aaaatactcg gatcgccggt catcccttcg
     1141 ttcatcgccc cgaagatccc ggtgccgctg gcgcaaaaca ggacaataat cgcgacataa
     1201 ttctgaataa aagattcatg cgctggcttc ttacgtgagt ggcgaaacag attttgtgct
     1261 ttggtgaccg cggtattgac gcctttttcc agcagacaaa tttcgccgat taatgcaccg
     1321 agtaaggttg ctaaaaccat cgccggaagg ttggcgcatt tcaccaccag taaaatacca
     1381 atccccagcg atgccagacc aaaaattgat gtcatggaga cgcggatacg ttccggtaag
     1441 cgttggctaa ggagtgcacc gagaacgcca cccagtaata ctgcacttgc gttgataaaa
     1501 ggaccgatga ccacaagagt tcctgttagc tgcattgtct gattttctta ttatgaccgt
     1561 ttggctcctt cgatggcttg tttgttcgca aagtcctggc ttgcacgctc tggtgaaagg
     1621 tgccatgatt gcgcgaattt tctcctctct gtacggagtt tgcccgatgc acgccacctc
     1681 cttacattct ctcgcttatc gccgtttcgc gcgaaactcc tccccttttc tgctcatgcg
     1741 gtgaagttaa cgcacgctca ctgcaggaca acagtaaaat cagagcgttt ctgcttttac
     1801 tgatgtctgg cggtcggagc tggtgaccag tttgacccat atctcgtggg gcagggtttt
     1861 tcaccttgtc cggtattctt acttccccga aacgggtttg cgcttatgaa atcaatgaat
     1921 attgccgcca gtagtgaact ggtatcccga ctttctactc atcgccgcgt ggtggcgttg
     1981 ggagatactg attttacgga cgtcgcggca gtcgtcatta ccgctgcgga tagtcgcagt
     2041 ggcattctta cgttgcttaa gcgcaccggt tttcatctac cggtgttttt gtattccgaa
     2101 catgttgttg aattacctgc gggcgttacg gcggtaatca acggcaacga gcagcagtgg
     2161 ctggagctgg aatccgcagc ctgtcagtat gaagagaatt tgctgccacc gttttatgac
     2221 acgctgacgc agtacgttga gatgggcaac agcacttttg cttgccctgg gcatcaacat
     2281 ggtgcgttct ttaaaaagca tcctgccgga cgccattttt acgatttctt tggtgagaac
     2341 gtctttcgca ccgatatgtg taacgctgac gtaaaattgg gcgatctgct tattcatgaa
     2401 ggatcggcga aggatgcgca gaaattcgca gccaaagtct ttcatgccga taaaacctat
     2461 tttgtactga acggcacatc ggcagcgaat aaagtagtga cgaatgcgct gttaacgcgt
     2521 ggcgatctgg tgctcttcga ccgtaacaac cataagtcga atcatcacgg cgcgctgatt
     2581 caggcggggg cgacgccggt ctatctggaa gcttcacgta acccgtttgg tttcattggc
     2641 ggtattgatg cgcactgttt taatgaagag tatctgcgcc agcaaattcg cgacgttgcg
     2701 ccagaaaaag cggagttgcc gcgcccgttt cgcctggcga ttattcagct gggaacttat
     2761 gacggcactg tctataacgc ccgtcaggtg gtcgataccg ttgggcatct gtgtgattac
     2821 attctgtttg attccgcgtg ggtcggttac gaacagttta tcccgatgat ggcggatagc
     2881 tcgccgctgc tgttagaact taacgaaaac gatccgggaa tctttgtgac ccagtcggtg
     2941 cacaaacagc aggcgggatt ctcacagacg tcgcagatcc ataaaaaaga taatcatatt
     3001 cgtgggcagg cgcgtttttg cccgcataag cggttgaata atgcctttat gctccatgct
     3061 tctaccagcc cgttctatcc gttgttcgcc gcgctggacg ttaacgccaa aattcatgaa
     3121 ggagagagtg ggcgtcggct gtgggcggag tgtgttgaga tagggattga agcgcgcaag
     3181 gctattcttg cgcactgtaa gctgttccgc ccgtttatcc cgcccgttgt tgatggcaaa
     3241 ttgtggcagg attatccgac gtcagtgtta gccagcgacc gccgtttttt cagttttgag
     3301 ccgggggcga agtggcacgg ctttgaagga tatgccgcgg atcagtattt tgttgatccg
     3361 tgcaagctgt tactcactac accaggtatc aatgccgaaa ctggcgaata tagcgacttt
     3421 ggcgttccgg cgacgattct ggcgcactat ctgcgtgaga acggcattgt gccggagaag
     3481 tgcgatctca attccattct gttcttatta actccggcgg aaagccacga gaagctggca
     3541 caactggtgg cgatgctggc gcagtttgaa cagcatattg aggatgactc gccgctggct
     3601 gaggtgttgc cgagcgttta taacaaatat ccggtgcgct atcgcgacta caccctgcgc
     3661 cagttgtgtc aggagatgca cgatctttat gtcagtttcg acgtcaaaga cctacaaaaa
     3721 gcgatgttcc gccagcagag tttcccgtca gtggtgatga atccccagga tgcgcatagc
     3781 gcttatattc gcggtgaagt ggagttggtg cggattcgtg atgccgaagg gcgaattgcg
     3841 gcagaagggg cgttgcctta tccacctggc gtgctttgcg tggtacccgg agaagtctgg
     3901 ggtggggcgg ttcaacgtta tttccttgcg ctggaagaag gggtgaattt gttgccggga
     3961 ttttcgccgg agctgcaagg tgtctatagc gaaaccgatg cagatggcat gaaacgattg
     4021 tacggttatg tgttgaagta agaaaaaaac gggtcacctt ctggcgaccc gtttttcttt
     4081 gcgtaattag tggctcaccg tctgtgtacc tgtcgggaca cgaacgtgtt tatatttgaa
     4141 catcgccatg aacgcgaagg ccagaaccac ggagtaacca gcgaaaatca accatacggt
     4201 ctgccagtcg gtaatgccgt tttgggtgta catctcaaca actttaccgc tcacgatgcc
     4261 gccaaggata cagccgaagc cgttagtcat catcaggaac atcccctgcg cactggcgcg
     4321 aattgccggg ctaacttctt tttcgacaaa caccgaacca gagatgttga agaagtcgaa
     4381 tgcgcaaccg taaacgatca tcgacagtac cagcagcacg gtaccgaacg gagtcgggtc
     4441 gccgtaagca aacagcgcaa aacgcaggat ccacgccaca atactgatca tcattacgtt
     4501 cttaataccg tagcggctta agaagaacgg gatggtcaga atgaacaggg tttcagagat
     4561 ctgcgaaatt gacatgatga ttgacgcatg ctgcacgatg aagctgctgg caaacatcgg
     4621 atctttgtcg aagctgtgca ggaaggtgtt accgaacatg ttggtaatct gcagttccgc
     4681 gccaagcagc attgagaaga tgaagaagat tgccatacgc ttgtttttaa acagcgcgaa
     4741 tgcatcgagg cccagcaggg ttgtccagct ctgattcgct tgctgtttag caaccggaat
     4801 atgcggcaga gtcagggtaa acagaaccag aatggcggaa agtgctgcgc caatatacag
     4861 ctgcatgtgg cttaattcga agccagacag gctcaccacc cacattgcca tgataaagcc
     4921 gatggtgccc cagatacgga ttggcgggaa gtcagtaacg atatccatcc cggcattttg
     4981 caggcgatag taagagatgg tgttgattaa cccaagcgtt ggcatataag caaacgagtt
     5041 aatcaatatc acaaggaaca tcgcctccgg cgtcgtgacc tgggccgcca tgaacagcgt
     5101 gatagcgcca atggtgtggc aaatggcata tacccatttc gcacttaacc atttgtcggc
     5161 cacaatcccc agcagcgcag gcataaagac cgcggcgata cccagtgagc tataaactgc
     5221 gccaatagaa gcaccgtcaa acttcagggt aacaaacata taggagccga gggtcgtcag
     5281 ccaacttccc cacagacaga actgcagaaa agagaggatt ttcagctgca gcttaagatt
     5341 catgttaatt tcctcacatc gtgatgcgga tgagtgtttg aaaaagcaca tttgaccgtg
     5401 cgagatgatg cgattctatc aacttcgcgg actggttttt tgttacctgt ggcaaataat
     5461 tgcaaacatt cccaatttgc aaaacgaaat tgtgatgtgg ataacatttt tgcccctgag
     5521 catcgtcagg ggcggttagt ggaattatcg gcggcggtag gatttttgcg cggtattcac
     5581 tttataaaga taacggcgag attccgcaga gggatggcgg gtcgtcagcg tttgataaac
     5641 atcgcccggc gtcatggtgt taataatatt ggccgcctga atcttgtcat tcgaaaagac
     5701 tcgcagcacg ctgcctgcgc cgccgttata ggcggtgatg acggcataac gccgggatgt
     5761 agggttatca attccgccga gataaacgtt gttcagcatc gcgagatacg cggtgccggt
     5821 atcaatattg ctggcaggat caaacaagaa actgcggctc ggcgtgccgg atttcccctg
     5881 cgaacggaac acatctttcc cggcagtatg ttgtaccacc tgcattaacc ccagcgcatc
     5941 ggaacggctg accgcatacg ggttaaagga agattcagtc tgcataattg ccagaatcag
     6001 cgactcatca atgccatatt tccgtgacgc ctggcggacc atgccgagat atttgtgcgc
     6061 acgtttatca aggtggttcg gcaccatgtt aatggtgatg ctatagatga ttcgtagccc
     6121 gttgctacgg ctttttagac ggtttttcag cagataatcc gcgaagttgc tcgcgcgacc
     6181 ttcccagcga atcggctgcc cggtgttgtc caccacctga ccgtaaagga aaggttcttt
     6241 cgaaatcgta atatcatcaa catcggaata gagatcgacc gaacccggat cgtcacccat
     6301 cagcaacgtt ttgataattg cccggcgcag gtgcgcggca ggttctgtcc cggcgatggt
     6361 ttcgatagtg atcgtaccgt catcgaagtt gatgtggctg cgggtctgat attgatcggt
     6421 gtatttcacg tagtccttag gaccggcgat caccacctct ttgaagcccc agatgttctc
     6481 aatattgtgg gcaaattgcc ccatcagaat atcaaaaccg ttggtatctt tgacccaggc
     6541 ttcgttatag gtatcgcctt ttttggtcgt cgaacaggag atgagcaacg gcgcaatcaa
     6601 agccagcgcg agatattttt tcatcattcc gggtgcgtgt tgtgtttgtt tttatgcaag
     6661 ttgccggagg cgtgctccgg cactgttttt attttttatc ttccggcgta tagccctcga
     6721 tatgcacctc tttaccctcg aacaggaagt tgaccatctc ctgttccagc agcttgcggt
     6781 gctcggcatt catcatgttg agtttctttt cattaatcag catggtctgt ttgtgctgcc
     6841 actgcgccca ggcttctttg gagatctcgt tatagatgcg ttttcccagc tcgccggggt
     6901 acagctgaaa atcctgacct tctgcttcac gttgcaggaa agtacaaaaa atcgttctgc
     6961 tcataaatca tcctctttat cgactcacgc gctaaaccgg cgcgccagtg cgtaactgct
     7021 gtaacaaacg ctccacggga gccgccaggc caactgacgg cggttgcgct aagttatacc
     7081 agagcgcatt gccttcatcc atgcagccgg tgaatgacga cacgggaagc cacataggca
     7141 caatatctaa gtggaaatgg ctgaaggtat gccgaaacgc ggtcagctgc gtcaggttat
     7201 cggcagcaat ctgccgttgc gccagccact gccgcaaact ttcttcgtcg gcaaactgcg
     7261 ggaaacagta taaaccgccc cacaatccgc tcggcggacg ctgcgccagc aatacttcat
     7321 cttcgtgctg taacaggaga aagtagccgg tgcgctccgg cagcgtctgc tttggttttt
     7381 tgcccggata aagcgaccag ctattgttgg cggcggcaat acacccgttt tgtagcggac
     7441 agagcgaaca tttcggcttc gagcgcgtgc aaatcatcgc gcccaaatcc atcatcgcct
     7501 gattaaaccg atccacgccg accgcgggcg tcacctgctc gcttaaactc cacagtttat
     7561 tctcgacctc ttttttccca ggccagccgc ttacagcata gcagcgcgcc agcacccgtt
     7621 tgacgttacc gtcgagaatc ggaaagtgct tacccagaga aagcgagaga atcgcgcctg
     7681 cggtggaacg cccgacgccc ggtaacgccg cgacttcttc aaaggtttcc gggaatttac
     7741 cgctgtgtaa ggtggccact tgttgtgccg ctttatgcag attgcgcgcg cgggcgtaat
     7801 agccaagccc ggtccacaag tggagaactt cgtccagcgg cgcattggcg agatcggtca
     7861 ccgttgggaa gcgcgccata aagcgttcaa aataggggat aacggtcgca acctgagttt
     7921 gttgcaacat cacttctgag agccatactt tgtagggcgt cttgtcaatt tgccagggca
     7981 gcgttttccg cccgtattta tcgtaccagt ccagaacctg ggctgaaaat tgcgacgctt
     8041 gcatggtcac cgaattcact gttgttgggg gcaagattgc agcacagcga cggcagggtg
     8101 taaaccggaa ctttccgcag ccacggcctt atcatgcttg catccggcaa ctaactttgg
     8161 ataatgcccg ttttcagaac actttcacaa gcgactaaat ctttatgaaa aacgacgtca
     8221 tctcaccgga atttgatgaa aacggccgcc cactgcgccg tatccgtagt tttgtgcgcc
     8281 gccaggggcg actgaccaaa ggccaggaac atgcgctgga aaactactgg ccggtgatgg
     8341 gcgttgagtt cagcgaagat atgctggatt tccctgtgct ttttggccgt gaagcgccgg
     8401 tgacgcttga gattggtttt ggcatggggg cgtcgctcgt ggcaatggct aaagatcgcc
     8461 ctgagcagga cttcctcggc attgaagtgc attcaccggg cgttggtgcg tgcctggctt
     8521 ctgcgcatga agagggctta agcaacctgc gcgtgatgtg tcacgatgcg gttgaagtgc
     8581 tgcataaaat gattcctgac aattcattgc gcatggtgca gctctttttc cctgacccgt
     8641 ggcacaaagc gcgccataat aaacgccgta tcgttcaggt gccgtttgcc gaactggtaa
     8701 aaagcaaact gcaactgggg ggcgtattcc atatggcgac cgactgggaa ccttatgcgg
     8761 aacatatgct tgaagtgatg tcttctattg acgggtataa aaacctgtca gagagcaatg
     8821 attacgtacc gcgtccggca tcacgtccgg tgacgaaatt tgaacaacgt ggtcatcgtc
     8881 ttggtcacgg agtatgggac ttaatgttcg agagggtgaa ataatggcaa agaaccgtag
     8941 ccgtcgtctg cgtaaaaaaa tgcacatcga cgaattccag gaattaggat tttcggtggc
     9001 atggcgattc ccggaaggta catcggaaga acagattgat aaaaccgttg atgactttat
     9061 taacgaggtt atcgaaccga acaaactggc ctttgacggc agcggttatc tggcctggga
     9121 aggtctgatc tgcatgcagg aaatcggcaa atgcaccgaa gaacatcagg cgattgtgcg
     9181 taagtggctg gaagagcgca aactgggcga ggtacgcacc agcgaacttt tcgacgtttg
     9241 gtgggactaa gaaagcatac gggagatgac aaatgcaaaa ctgcctgatg cgctacgctt
     9301 atcaggcctg gaaagatgca caatcgagta ggacggataa ggcgtttacg ccgcatccgg
     9361 catggatgac atgtactttg ttatcaatct ggggccagca aatgctggcc tgatttgttc
     9421 ttgagggaag actatgatgc gcaaaatgct gctggcggca gcactttcag tgacggcgat
     9481 gaccgctcac gccgactacc agtgcagcgt cacgccgcgt gacgatgtga ttgtcagccc
     9541 gcaaaccgtg caggtgaagg gcgaaaacgg caatctggtg atcacgccag acggcaacgt
     9601 gatgtataac ggtaagcaat attccctgaa cgccgcccag cgcgagcagg cgaaggatta
     9661 tcaggctgaa ctacgtagca cgctgccgtg gattgatgaa ggcgcgaaaa gccgcgtcga
     9721 aaaagcccgt attgcgctgg ataaaattat cgttcaggag atgggcgaaa gcagcaaaat
     9781 gcgcagccgt ctgaccaaac ttgatgcgca gctgaaagag cagatgaacc gcattatcga
     9841 aacgcgcagc gatggcctga cgttccacta taaagccatt gatcaggttc gcgccgaagg
     9901 ccagcaatta gtgaatcagg caatgggcgg aattttacag gacagcatta atgaaatggg
     9961 cgcgaaagcg gtgctgaaaa gcggcggtaa cccattacag aatgtgctgg gaagcctggg
    10021 cggtctgcaa tcctcaatcc aaaccgaatg gaaaaagcag gaaaaagatt tccagcagtt
    10081 tggcaaagat gtttgtagcc gcgttgtgac tctggaagat agtcgcaaag ccctggtcgg
    10141 gaatttaaaa taatcctcta ttttaagacg gcataatact tttttatgcc gtttaattct
    10201 tcgttttgtt acctgcctct aactttgtag atctccaaaa tatattcacg ttgtaaattg
    10261 tttaacgtca aatttcccat acaaagctaa gggataatgc gtagcgttca cgtaactgga
    10321 ggaatgaaat ggagtttttc aaaaagacgg cacttgccgc actggttatg ggttttagtg
    10381 gtgcagcatt ggcattaccc aatatcacca ttttagcaac cggcgggacc attgccggtg
    10441 gtggtgactc cgcaaccaaa tctaactaca cagcgggtaa agttggcgta gaaaatctgg
    10501 ttaatgcggt gccgcaactg aaggacattg cgaacgttaa aggcgagcag gtagtgaata
    10561 tcggctccca ggacatgaac gataatgtct ggctgacact ggcgaaaaaa attaacaccg
    10621 actgcgataa aaccgacggc ttcgtcatta cccacggtac cgacacgatg gaagaaaccg
    10681 cttacttcct cgacctgacg gtgaaatgcg acaaaccggt ggtgatggtc ggcgcaatgc
    10741 gcccgtccac gtccatgagc gcagacggtc cattcaacct gtataacgcg gtagtgaccg
    10801 cagctgataa agcctccgct aatcgtggcg tgctggtggt aatgaacgac accgtactgg
    10861 acggtcgcga tgtcaccaaa accaatacca ccgacgtagc gaccttcaag tctgttaact
    10921 acggtcctct gggatacatt cacaacggta agattgacta ccaacgtacc ccggcacgta
    10981 agcacaccag cgatacgccg ttcgatatct ctaagctgaa tgagctgccg aaagtcggca
    11041 tcgtttataa ctacgctaac gcatccgatc ttccggctaa agcactggta gatgcgggct
    11101 atgatggcat cgttagcgct ggtgtgggta atggtaacct gtataaatcc gtgttcgaca
    11161 cccttgcaac cgccgcgaaa aacggcactg cagtagtgcg ttcttcccgc gtaccgacgg
    11221 gcgctaccac tcaggatgcc gaagtggatg atgcgaaata cggcttcgtc gcctctggca
    11281 cgctgaaccc gcaaaaagcg cgcgtcctgc tgcagctggc tctgacgcaa accaaagatc
    11341 cgcagcagat ccagcagatc ttcaatcagt actaatcacc tcgccccggt aacgtgccgg
    11401 ggctttttca cttcagactt acgtccattg ccaattttca tcaccctaat gataatcacc
    11461 agaataaatt attccgcgcg agggttttcg ggtgaaaaag caatggattg ttggcacggc
    11521 gctgcttatg ttgatgactg gtaatgtctg ggcagatggc gaaccgccaa ctgaaaatat
    11581 cttaaaagat caattcaaaa agcagtatca cggcattctc aagcttgatg ctatcagctt
    11641 aaaaaatctt gatgctaagg gtaatcaggc cacctggtca gcggaaggcg atgtctcttc
    11701 cagtgacgat ctctatacct gggtcggtca gttggcagat tacgaactgc tcgaacagac
    11761 ctggacgaaa gataaaccgg taaaattctc ggcgatgtta accagtaaag gaacgcccgc
    11821 gtctggctgg tcggtgaact tttactcttt tcaggcggca gccagcgatc gtgggcgggt
    11881 ggtcgacgat atcaaaacga ataataaata tctgatcgtg aatagcgaag atttcaatta
    11941 tcgctttagt cagcttgagt ctgcgttgaa taaccagaag aactcgattc ctgcgctgga
    12001 aaaggaagtg aaagcgcttg ataagcaaat ggtggcggcg cagaaagcag cggatgcata
    12061 ttggggaaaa gatgcaaacg gtaagcaaat gacccgcgaa gacgccttta aaaaaattca
    12121 ccaacagcgt gatgagttta ataaacagaa tgatagcgag gcgttcgcgg ttaaatatga
    12181 caaagaggtt tatcaaccgg cgattgcggc atgtcataaa cagagtgaag agtgttatga
    12241 agtgtcgatt cagcagaagc gagatttcga tatcaacgaa caacggcgac agacttttct
    12301 gcaatcacaa aaacttagcc gtaaattgca ggatgactgg ataacgctcg agaaagggca
    12361 atatccgtta acgatgaaag tttcagaaat aaacagtaaa aaagtcgcga ttttgatgaa
    12421 aattgacgat atcaatcagg ctaatgaacg ctggaaaaaa gataccgaac aattgcggcg
    12481 caatggagtg attaagtaac tcatgttaag tacctttgcc ggataatata aaaagttatc
    12541 cggcaaatta ttttttacat tagccctagt agctgtggaa tgaacaagga tatttgcggg
    12601 atgtaagtga tcatcatcaa cgtgattaac aatacggcat aaaatggcag caatggtttg
    12661 atcaaatgtt gaatcttcac acctgaaata gaacaaccaa caaatagcgc actacccacc
    12721 ggcggtgtta acagcccaat acaaaggttg gccaccatca taataccgaa atgtaccgga
    12781 tccattccca gctcttgtgc aatcggcaag aagattggtg tgaagattag tacagccggt
    12841 gtcatgtcca taaagatgcc gacaattaat aagacgatat taatcagcag caatatgatc
    12901 agtgggttat cagaaatccc catcaatgcg tcgctgatca tataaggaat atcagcattg
    12961 gtcattgccc aggacatgcc cacggaaaaa ccgatcaaca gtaatacaat cgaagtggta
    13021 acaaccgatt ccagaatcag tttgggtaaa tcgcgccatt ttacttcccg gtaaatcagc
    13081 accgagagaa taaatgtgta gacaacggca attgctgacg cttcagttgc ggtaaaaatg
    13141 ccgcccagaa tgccgcccat tacaatgaac accagaagca aacttggtaa ggcatccaga
    13201 aacgctttac atgcctgagc aaaggttgct ctttctgaaa gtgggtagcc acggcgttta
    13261 gcaataatgc cgcagactat cattaccgca agacccatta agatccctgg caaatatccc
    13321 gccataaata acgatgctac agaaacgcca ccagctgtca gggaaaacac aattaacacg
    13381 tttgagggcg gtatcagtaa tccagtaata caggatgaga cgtttacagc ggtagaaaat
    13441 gccggatcat aaccttcttt ggtctgaatg ggattcagtg tgccaccaac ggcagcagct
    13501 gccgcaacgg ctgaaccaga aatggaacca aacatcatat tcgccaggac gttcacatga
    13561 ccaagcgaac caggaacgcg gccaaccatt acctgggcga ggttaatcaa acgtatcgca
    13621 ataccgccgc tgttcatcag cgttccggca aagatgaaaa aggggattgc caacagggca
    13681 aagttatcca gtccattagc cagtcgttgc gaaacagtga ttgccgcgat atcccaggga
    13741 aacatcaaaa gcattgaagc tacagtggca ataccgattg agaacgaaat gggcacacca
    13801 ataaagacca gcacaaaaaa acagccaaac agtgtaatcg cgatataact atccattaac
    13861 tggcctcctg cgcaggttgt ttcagttgtt gaatgctttg attaataaaa cacaaggcat
    13921 aaaacatcat caccaatcca cttaatggca gaatgatata tacgtatccc atcggtattt
    13981 gcatcgcggc agatacctgt gatgttgcca gtgttttatc aattaatttc aggccaccag
    14041 tcacaatgac cgaaccggca aaacctaaaa taagaacgtt aatgactata ctaagaagta
    14101 attgcttgcg cttattcagg gcgagagcaa ataaatcgat agagagatgt ctctgcgcgc
    14161 cgacggtata tgcagcacct aataatccta cccagatcat caaaaagcgt gccagctcat
    14221 cggttaatgt acttggctgg ttaagtacgt agcggctaaa tacttgccag actacgcata
    14281 caaccagagc aagcattacg gcgacagaaa acgcggcaat gacgcggtct accgcaagtt
    14341 tcaggcggtt aaatatcatt gtaagcatcc tggaaaatca gggccagaga ctggcccgaa
    14401 atattattga gcagcgttgt cgaatttctc taacaaagca gcttgttttg gatctttttt
    14461 gaagtcgtca aacagaggtt gaactgcggc acggaatggg gctttatcaa ccttaacgat
    14521 ttcaccaccc atagctttcg cctgagcacg tgtgtcagcg tcaatttttt cccacaattt
    14581 ttgttgataa gcttcggatt tcttcgccgc tgtttcgagg atttgttgct gttctggggt
    14641 taatttattc catgttttgg tggagataac caaaaagtca ggaattgagg catgttcatc
    14701 ttcagaaaag actttggcaa tttcgatatg tcgagtctgc acccaggaag gcacgttatt
    14761 ttctgcgcca tctactacac cttgctgcat cgcagtataa acttcgccaa atgaaattgg
    14821 agtgggtgag ccacccatca gttcaatcat tttgatggtg gtcgggcttg gctgaacacg
    14881 aattttcagc cctttaagat cttctggctt agtaatcggt tttttcgcat agaaactgcg
    14941 tgtacccgct acataagctg ataaagcaaa aaagccttta tcttttgttg aatccataat
    15001 ttctttacca acttcaccaa aaaccacttt gttgaagtgc gcctgatctt taaataagaa
    15061 cggcaaatta tagatagcat aaatattatc aaaagactcc agatcactgg cggaaccttt
    15121 ggtcatatcc agtgcaccat tttgcaacaa ctccatagtt tctcgcgcat tacccatctg
    15181 gctgctgggg taaatacgga tcaccatatt accttttgac agctgtttta cttctttcgc
    15241 cagctcttca aaactctgat gtacaacatg acttctttcc aggttgtgtg ctaactttaa
    15301 cgtcactttt tctgcggcgt ttacgccagt gctaaccaga gccagcaagg atgcgcagac
    15361 taatgagcgg gtaaatgacc tcattttcat aaaaaaactc cctgttgaag aatggaaatt
    15421 ggttgatatc taattgtgct tatcagtgct ttgtgcgaca aattggttaa cctgtctgac
    15481 aacttgtcta tgtttatcac aacttgaaat aaagtcagaa attaaatatt aattttgtgc
    15541 gccaggttgt acatgtttat aatcaataaa tgatgagtaa atattcacta aatatatata
    15601 ataaatatcg tcgatattta ttcagtaaga aatatatttt ttccgaagtt aattaacggg
    15661 gggttatgag aagtgattaa tgcgcccgca ggcgcattaa tgattactca gcgaggaata
    15721 attcaagaag tgagtttaag aataatttac catgctcggt tatctgccag taatccgcac
    15781 attctgtgag ataaccttga gcgatagcct cgtctaactg agagcgaata acttcttcag
    15841 aaaggccagt atattggcta aactccacgc gcggcgcggc ctcaagcaga cggaagcgat
    15901 tcataaagaa ctcaaacggt ttatctgcag cttcgacatc acgctggctt tccagatatc
    15961 ttccctgcat aaaaccacgc ggatgacgcg ttttggtggt acgcagaatg cgaccatccg
    16021 ggaaggtgac tttgccgtgc gcgccgcagc caataccaat gtagtcgcca aatcgccagt
    16081 agttgagatt gtgctggcac tgataacctg gtttggcgta agcggatgtt tcatactgct
    16141 gatagcccgc tgcggttaat aactgatgcc cctgttcgaa tatatcccac aacgcatcgt
    16201 cgtccggcag taccggcggg cgagagccaa acagcgtatt aggttcgatg gtcagttgat
    16261 accaggaaag atgcggcgga ttcagttcaa tagcctggcg cagatcgcca agcgcctctt
    16321 ccagtgattg atccggcagc ccatgcatca aatcaaggtt aaaactacgc agccccagcc
    16381 cgcttgcaag ttttgccgct cgtttcgctt cttgcgggcc atgaatgcgc ccaagtcgtt
    16441 tcagcttttc ttcgctaaaa ctctgcacac caatagagat gcggttcaca ccagcacgct
    16501 gataatcaac aaagcgattg gcttctaccg taccagggtt cgcttccata gtaatttctg
    16561 catccgctgc cagcggcaaa cgcgcacgca cgccgtccag cagcgtttgc atcgccgggc
    16621 cggaaagcag gctcggcgta ccaccgccaa taaagattgt ctgcacttca cggccctgag
    16681 cgtaagccac atcgttgtcc agatcgttaa gcagatgctg aacataatcg tcgtgcggca
    16741 cttctccttt caacgcgtga gagttgaaat cgcagtacgg gcatttctgc acgcaccacg
    16801 ggatgtgaat gtagagactc agcggcggta atttaaccat tacgtaaagc gtccagcagc
    16861 agtttcaatg cctgaccacg gtgggaaatg gcgctctttt cttcgcgggt cagttcggca
    16921 gcggttttcc cttcggaagg aacgaagaaa attggatcat aaccaaagcc accagtgccc
    16981 gctggttcac gagtaatcac acccggccag ctgccgtggc acaccagcgg agtgggatct
    17041 tccgcgtgac gcagatatac cagcacgcag tggaaacgcg cctgacgttg gtcgtccggt
    17101 acgtctttca gtgtttccag caatttttgc agattctttt gatcggtcgc gtcttccccg
    17161 gaataacgcg cggagtaaat gcccggcgca ccacccagca catctaccgc cagaccagag
    17221 tcgtcggcaa ttgccggtaa accggtcact tttgccgcat ggcgcgcttt cagaatcgcg
    17281 ttttcgataa aggtcaggcc agtttcttca gcggaatcta cgccgagatc tgtttgggcc
    17341 acgatatcaa gaccgaagtc gctaagcagc gacgccagct cacgcacttt accggcattg
    17401 ccggttgcga ggacaacttt ttgcatagga tacctaatta attaacgccg cgatttctgg
    17461 cgggatttgt tgcggattaa tgattttaat ttgtttgtgg cggccaagtt cgcctttttc
    17521 aattaccacc tggcttttgg ctacccggaa ttgcttaccg agaaacttca ccagatggct
    17581 gttggcctgg ccgtcaaccg gcggcgcggt aatggcgact ttaacttcgt cgccatgtaa
    17641 accgacaata gaatcacggc tggctttcgg ctgaatatag agccgtaaaa ccagaccgtc
    17701 atcattaact gttacggcac tcataacgcc atccacagcc ccggcagcag catgtttccg
    17761 gttgcctgta atacttctgc gacacccata ttgatgacat acagcaacag aacgaggatc
    17821 atcggcgaga aatcaatccc acccattgcc ggtagcaggc ggcgaatcgg gcgcagcagc
    17881 ggatcggcca gctgaatcag cacgtattca atcgggctac gcccctggct tacccagctc
    17941 ataatcgcca tcaccagcag gacccagaaa atcagcaggc cgatggtttt cagcagaatc
    18001 agtaaaccgg caatccaaat gattggcaga aaggtcacca ctttaaacag cacgatggct
    18061 ttgatgaaac tgagaatgta cgcaaccagc agtgaggcgc tgtcaattgg ccccattgcc
    18121 ggaataacgc ggcgcagtgg cccgataatt ggctgcgtta ccttcactac aaactgtgag
    18181 aaggggttgt aaaaatcaca atgagcccac tgcatccaga tgcgtaataa cagcaccatg
    18241 gtatacagct caatgaccgt tgaaagcagg aaagtcaacg tattcatggc gttcctttaa
    18301 ttccttattt tttagagtaa tcacgcgcac caaaaattgc agtgccgata cgaaccatcg
    18361 tgctacctgc cgcaatggcg gcttccatat cgtccgacat tcccagagag agcgtgtcga
    18421 tatgcgggta gcgcgttttc agtccggcaa atgctacagc catttggcgt gcaacttcaa
    18481 actgccttac atattctgac tcaggcgcag ggattgccat cagcccgcgc agacgtaaac
    18541 tcggtagttc agcgaccgca gctgccagct cgtccagttc tgccagttga atcccggact
    18601 tactgttttc atcactaatg ttaatttgaa tcagtacgtt aagaggggga agttctgccg
    18661 ggcgctggtc gttcaggcgg gtagcgatgc gcaaacggtc gatggtatga caccagtcga
    18721 aatgctctgc caccaggcgg cttttattag actgcaacgg gccaataaaa tgccattcta
    18781 atcctgttac gcccagttcc tgaaagtggc gaattttatc taccccttcc tgaacgtagt
    18841 tttcaccaaa ttgacgctgc ccggcatcaa tggcttctgc gatggcgctc gcaggttttg
    18901 ttttactgac tgcaagcagc gtaatttctt ctggagaacg gccgcaacgc gttgcagccg
    18961 ctgagatttt gtcccggacc tgtgccaggt tatgcgcaat atcgttcatt ttccgaggat
    19021 cttagtatga atatggaaga aattgtggcc cttagtgtaa agcataacgt ctcggatcta
    19081 cacctgtgca gcgcctggcc cgcacgatgg cgtattcgcg ggcgaatgga agctgcgccg
    19141 tttgatgcgc cggacgtcga agagctactg cgggagtggc tggatgacga tcagcgggca
    19201 atattgctgg agaatggtca gctggatttt gctgtgtcgc tggcggaaaa ccagcggttg
    19261 cgtggcagtg cgttcgcgca acggcaaggt atttctctgg cattacggtt gttaccttcg
    19321 cactgtccac agctcgaaca gcttggtgcg ccaccggtat tgccggaatt actcaagagc
    19381 gagaatggcc tgattctggt gacgggagcg acggggagcg gcaaatctac cacgctggcg
    19441 gcgatggttg gctatctcaa tcaacatgcc gatgcgcata ttctgacgct ggaagatcct
    19501 gttgaatatc tctatgccag ccagcgatgt ttgatccagc agcgggaaat tggtttgcac
    19561 tgtatgacgt tcgcatcggg attgcgggcc gcattgcggg aagatcccga tgtgattttg
    19621 ctcggagagc tgcgtgacag cgagacaatc cgtctggcgc tgacggcagc agaaaccgga
    19681 cacctggtgc tggcaacttt acatacgcgt ggtgcggcgc aggcagttga gcggttggtg
    19741 gattcatttc cggcgcagga aaaagatccc gtgcgtaatc aactggcagg tagtttacgg
    19801 gcggtgttgt cacaaaagct ggaagtggat aaacaggaag gacgcgtggc gctgtttgaa
    19861 ttgctggtta acacacccgc ggtggggaat ttgattcgcg aagggaaaac ccaccagtta
    19921 ccgcatgtta ttcaaaccgg gcagcaggtg gggatgttaa cgtttcagca gagttatcag
    19981 cagcgggtgg gggaaggacg tttgtgaggc taaagtgatt gattagcttc gtaaatatgc
    20041 ctgatgcgct acgcttatca ggcttacaaa tgtagtcatt cacgccgcat ccggcaataa
    20101 ggagcgatga cgtaaatcgc cttaatatcc ctgctcgaaa taactttcga gaatgataac
    20161 cgcagaggca gagtcaactt tgcctttgtt gagcgcccga tagccgccct gttcaaacag
    20221 accggaacgg gcttccacag tgctaagacg ctcgtcatgg agctttactt caacaccgaa
    20281 acgaccatga atacggttgg caaatttgcg cgctctggcg gttaacggct gttcggtgcc
    20341 gtccatattc agcggcaaac cgacgatgat ttcgtcaggc tgccactctt tcagtaaacg
    20401 ttcgatgagg ttccagtccg gcgtaccgtc ctgcgcttta attgcaggca aagggcgagc
    20461 ggtgccggta atgcgttggc cgaccgctac gccaatgctt ttggtgccga agtcgaaggc
    20521 gagtaaggtt ccactcatca ggcgtgccct gccacaccag gcatggtgag aatatccaca
    20581 ccaatcagtt ttgccgcctc gcgccagcgg tcggcaatcg gtgttttgaa cagaatattc
    20641 agatccgccg gggccgttag ccacgcgtta tcgagaattt cttgttccag ttgacctttc
    20701 tcccaggagg catagcccag agccaccaac acgtcagacg gttgtttatc ggtgccgagc
    20761 gtttccagca catcgcggga agtggtcatc accgtgttgt ctgaaatgcg aatgctggaa
    20821 gcaaaattgg agggcggagt atgcaaaata aacccgcgat cttcagccag cggaccgccg
    20881 agcataaccg gtttatccag acggattgat tcatcacgcg gctccggcgt gatcttcagc
    20941 ttttccagaa tcccttcaat tttgagattt tccagcggct tgttgacgat gatccccatt
    21001 gcaccattgg tattatgttc gcaaatgtag accacggaac gacggaaaat cggatcctgg
    21061 agagcaggca tggcaataag aaagtgatgc tgtaaattca ttgtcagagg ttctgtttcc
    21121 tggttcaaaa aagcaacagc gcccagtatg cgtggaaaga agggcgctgt cactcagagt
    21181 ctcaacgaga tccttctcgc taaggtgggt tactgctgct gtaaacgtgc ttcgatggca
    21241 tccattaaca ttccggtgat cgacaccgga aactctgctt caatctcacg aatacaggtt
    21301 gggctggtga cgttaatttc agtcagacgg tcgccaatga tatccagacc aacaaaaatc
    21361 agcccttttt ctttcagtgt cggcccgatc tgacgggcga ttttccagtc actttccgtc
    21421 agcggacgag gttcaccgcg accaccggca gccagattgc cacgggtttc gcccccctgc
    21481 ggaatacgcg ccaggcagta cggcaccggc tcgccatcca ccaccaacac gcgtttgtcg
    21541 ccatctttaa tggctggcag gtaattttgc gccatgcagt agcgagtgcc atgctcagtc
    21601 agggtttcgg caatcacgcc gaggtttgga tcgccttctt tcacgcggaa aatcgacgcg
    21661 ccgcccatac cgtcaagcgg cttaagaatg atgtcgctgt gtttctccca gaacgctttc
    21721 agctgtgctt tattgcgcgt aaccagcgtt tctggcgtta agtcagagaa ccaggcggta
    21781 aacagtttct cgttacagtc gcgcaggctc tgaggcttgt taacgatcag cgtccctttc
    21841 tcttcggcac gttccagaat ataggtcgcg tagataaact cggtatcaaa cggcgggtct
    21901 ttacgcatca ggatcacgtc gagatcggcc agcggcagat cctgttcacc gacgaacgaa
    21961 aaccactctt cgtagttctg cttcacgttc agcgtgcggg tatgggcgcg ggcttcgcca
    22021 ttgatcagat acagatcgcc catctccata tagtgaagtt cgtaaccacg acgctgtgct
    22081 tccagcaaca tagcgaaact ggaatctttc ttgatgttga tgtttgcgat ggggtccatc
    22141 acgatgccga gcttgatcat tattcttctc cgttagccca aatcgccaaa tcgtacttgt
    22201 agcgcggtaa tggcggtgag cgcagttgtc tctgtacgca aaacgcgagg tcccaacagg
    22261 atatcagtaa attgatagcg ggcagtcatg gcaatttcat ctgccgataa accgccttcc
    22321 gggccaatca gcaggcggac gcgttcaacc ggtaacggca acgtattgat gctgttactg
    22381 gcgcgcgggt gaagattcag tttcagtcct tcatcctgct ctgcacacca ggcttccaga
    22441 tccatcgctg gacggatttc cggcacccgg ttacgaccac actgctcaca ggcagcaatt
    22501 gcaatcttct gccactgctg gagcttcttg ttcagacgtt cactatccag tttaacgccg
    22561 cagcgctcag aaaaaagtgg cgtaatgagg cttacaccga gttcgatcga tttctggatc
    22621 gtaaattcca ttttttcacc acgcgacatc acctgaccga ggtgaatatg cagcggagat
    22681 tcgcgatcgt cgatctggcc ttccagcacc ttcacttcca cgcttttttt gctggcgctg
    22741 gtaatttcgg cgtcaaagac ctggttgcta ccgtcaaaca attgcaacgc ctgtcccggc
    22801 cccatacgca gtacgcgccc gatatggttg gcggcatctt cgcaaagcgc gatgtgagaa
    22861 tggctggtca gtggttcagg atgataaatg cgggggatac gcatagttaa aaatccgcgt
    22921 cgtctcccca cgcggttgta cgcgtggggt aggggttaac aaaaagaatc ccgctagtgt
    22981 aggttagctc tttcgcgcct ggcaagcgcg ttgcacatac gggttatgat tgccctgcac
    23041 cttcgcgatg cgttcatcgc gctcgcactc ccagtcggta accggataca tcttgtccca
    23101 tgcgttgaac agttgcgttt gctggcgaga gagtgtcagg ttgtattggt cacgcatata
    23161 gaagtaggtg cgcgcaatgg caccgcgtgc acgcgctggt ggttcggcgg ctttttcttt
    23221 gaaatcgacc ttcatggcgc attgaccgta ctggccttcg ccgccattcc actggctgta
    23281 cataaagttg ccgcgatcgc cattcacctc gccgactgac ggttgcaggt tatgcatatc
    23341 gctttccatc ttgcgataga ccggatcttt ggcgcagttt ttacgtccac cgtcctgcca
    23401 gcactggcgc tggtgaccga attgccaggc gggaacgacg tgttcccact ctacgcggct
    23461 ggcgcggttt tcatttttgc gcacctgata gccgcacgat tgcagatcaa caacgccttt
    23521 tttgccctgc cagttaattt tacatccgca ataaaacgta ccgggcgcgt cagcgtggac
    23581 ttttaccgcc gcggctttcg cctgagaaaa actattgata ccttcggcca acgccgggcc
    23641 ggaaaatgct gcgctcagta ccaccgcagc aatagacaaa taacggtaca tcacttactc
    23701 cgtgttaaaa cgagccagca acgtagcgaa agttgttttg gtatgcaatc agccagatca
    23761 ggaaagttcc tgatagttca gatggttatt ttgcgaccag ttgttcgccg cagtgaacac
    23821 agcgatagac ggcttcgcca cgaactacgc gattatggcg gcgtacggta agctgatgct
    23881 cctggcactt gcagcggtag gggaaggtgt tgcgacgcac ggattgcagt tcaaattgat
    23941 gcgtacgacg ggcgggaacg cccagcacgt tttccatcat ccacttccac tctttgccat
    24001 gtggcgctac gcggccgaaa tgtttccata ccagcaaatg tgccagttcg tgcggcacca
    24061 cttcttcaat aaaggcttca ctgttttcca acagcaaaac gggattgagg cggatttcat
    24121 agctttccag ccaggctgtt ccggcggagg tgccgcgctg ggtgtaagag agttttggct
    24181 ccgggtagtt acgccctagc ttcaggttgg cctgggcgag tttttcccgc aggcgacgca
    24241 taacggcctg ttggatggcg atagggagac gggatgtttt catagcggca gaggatagag
    24301 cgaagaaaag atgactgcaa gaggtggctt cctccgcgac gggaggaagc ctgaggagat
    24361 taatcgtgag cgccgatttc gcgcagttta cgacctttca tcagattacg ttcaatatgt
    24421 tccagcgaaa cgtgtttggt ttccggcacc agccacaatg tcagcaggat aaacagtacg
    24481 ttcagagccg catacaccca gaaggtgttg gcgttaccca gcgtgttgag catggtcagg
    24541 aacgttgcgc caacgatcat gttggcaatc cagttggtgg cggtggagca ggtgatgcca
    24601 aaatcgcggc ctttcagcgg ctgaatttcg gagcacagta cccaaatcag cggaccggca
    24661 ctcatggcaa agccaacaat aaacatcagc agcatggcga tagcgaaata ctgcgccgac
    24721 ggagagtgga taccgatatg catcattgta ccgagtacgc ccatgccagc agccatcacc
    24781 aggaagccca gcgttagcgt tggtttgcgt ccccagcggt caacaaggcc gattgcgata
    24841 aaggtggcaa gtacgttggt caggccgaca atcaccgtcc cccacatttg ctcggtagtg
    24901 ttggtataac ccgccagttc gaagattttc ggcgcgtaat acatgatgac gttcatcccg
    24961 gtgaattgct gcattacctg caatagtacg ccaaggaaca ccgcgcggcg gaagttgcta
    25021 ttctctttaa acagcgccca gccactctgt ttaacctgca aactttcacg gatttcatcc
    25081 agttcgcgtt tcgcttccgc gctggtgtca cgcaggcgta gcagcacgcg ttcggcatca
    25141 acaaaacggc gtttggcggc aaaccaacgt gggctgtctg gcaggaagaa gacaccaatc
    25201 agcagcaaaa ttgccgggat gataatcaca cccagcatcc agcgccatgc accggtgtag
    25261 ctgaaggcgg tatcagaaag ataagcgccg aggatcccga tagtgatcat caactgatac
    25321 atcgagatca tactgccgcg aattttttcc ggcgcaattt cagagaggta cagcggtgcg
    25381 gtataagagg ccacacccac cgccagcccc agcagaacgc gggaaagaat cagtacttca
    25441 acgtttggcg cagccgcaga gaacagtgaa ccggcaacaa acaagattgc gccgatcatc
    25501 aggctctttt tgcgcccgag tttaaaggag agccagccgc tgcccaccgc accgactgcc
    25561 gcaccgaaca tcatggagct tacgacccat tcttgcgtgt gcgaagtaat ctggaattca
    25621 tctgcaataa acggcagtgc gccagcaatt acaccgatat ccaggccaaa gagtaatccc
    25681 gccagagcgg caaggaagca gacgaaaaac gtcattgcct tgtttgaccg cccctgtttt
    25741 ttagcgtcag gcatgatgcc ctccaatatg gttatttttt attgtgaatt aagataggtg
    25801 agtacgacgt aaaaagatgt gaagcaaatc acatcagtgt aatcgcttac actaacattt
    25861 atcttggtta tgatttaagt gcatgaaaat aattgagtta tatcatcata gattaaacgc
    25921 t
//
LOCUS       DACBVI010000057        25871 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.057, whole genome shotgun sequence.
ACCESSION   DACBVI010000057 DACBVI010000000
VERSION     DACBVI010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.057"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            41..922
                     /gene="rihB"
                     /locus_tag="IU325_11440"
     CDS             41..922
                     /gene="rihB"
                     /locus_tag="IU325_11440"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HAP5809634.1"
                     /translation="MMAAKHPAIDLLGITIVAGNQTLDKTLINGLNVCQKLEINVPVY
                     AGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAESTHAVKYIIDTLMASDGDITLV
                     PVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFTPSAEFNIFADPEAARVVFTS
                     GVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSDIMNFTLKTQFENYGLAGGPV
                     HDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCDELGVLGKPANTKVGITIDTD
                     WFWGLVEECVRGYIKTH"
     gene            1022..2272
                     /locus_tag="IU325_11445"
     CDS             1022..2272
                     /locus_tag="IU325_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP5809635.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(2328..3416)
                     /locus_tag="IU325_11450"
     CDS             complement(2328..3416)
                     /locus_tag="IU325_11450"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP5809636.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNTAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3419..4276)
                     /locus_tag="IU325_11455"
     CDS             complement(3419..4276)
                     /locus_tag="IU325_11455"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP5809637.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVAEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(4350..5399)
                     /locus_tag="IU325_11460"
     CDS             complement(4350..5399)
                     /locus_tag="IU325_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HAP5809638.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            5498..6379
                     /locus_tag="IU325_11465"
     CDS             5498..6379
                     /locus_tag="IU325_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP5809639.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAIEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            6584..8053
                     /gene="lysP"
                     /locus_tag="IU325_11470"
     CDS             6584..8053
                     /gene="lysP"
                     /locus_tag="IU325_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HAP5809640.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            8346..10325
                     /gene="cirA"
                     /locus_tag="IU325_11475"
     CDS             8346..10325
                     /gene="cirA"
                     /locus_tag="IU325_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HAP5809641.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEDGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFTA
                     NGVPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLSDLPF
                     HTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVTKDVK
                     LRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(10357..11193)
                     /locus_tag="IU325_11480"
     CDS             complement(10357..11193)
                     /locus_tag="IU325_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase YeiG"
                     /protein_id="HAP5809642.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALIQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            11451..12119
                     /gene="folE"
                     /locus_tag="IU325_11485"
     CDS             11451..12119
                     /gene="folE"
                     /locus_tag="IU325_11485"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HAP5809643.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            12136..13293
                     /gene="yeiB"
                     /locus_tag="IU325_11490"
     CDS             12136..13293
                     /gene="yeiB"
                     /locus_tag="IU325_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HAP5809644.1"
                     /translation="MERNVTLDFVRGVAILGVLLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLMLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            13435..14475
                     /gene="galS"
                     /locus_tag="IU325_11495"
     CDS             13435..14475
                     /gene="galS"
                     /locus_tag="IU325_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HAP5809645.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            14755..15753
                     /gene="mglB"
                     /locus_tag="IU325_11500"
     CDS             14755..15753
                     /gene="mglB"
                     /locus_tag="IU325_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HAP5809646.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            15814..17334
                     /gene="mglA"
                     /locus_tag="IU325_11505"
     CDS             15814..17334
                     /gene="mglA"
                     /locus_tag="IU325_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HAP5809647.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            17350..18360
                     /gene="mglC"
                     /locus_tag="IU325_11510"
     CDS             17350..18360
                     /gene="mglC"
                     /locus_tag="IU325_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HAP5809648.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(18431..19666)
                     /gene="preA"
                     /locus_tag="IU325_11515"
     CDS             complement(18431..19666)
                     /gene="preA"
                     /locus_tag="IU325_11515"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HAP5809649.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEDNLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQIRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHSVTL"
     gene            complement(19660..20898)
                     /locus_tag="IU325_11520"
     CDS             complement(19660..20898)
                     /locus_tag="IU325_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP5809650.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGNTFTLDQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTINTQNYQTRNPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(21295..21534)
                     /locus_tag="IU325_11525"
     CDS             complement(21295..21534)
                     /locus_tag="IU325_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HAP5809651.1"
                     /translation="MDVQHFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(21537..22256)
                     /gene="sanA"
                     /locus_tag="IU325_11530"
     CDS             complement(21537..22256)
                     /gene="sanA"
                     /locus_tag="IU325_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HAP5809652.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(22406..23290)
                     /gene="cdd"
                     /locus_tag="IU325_11535"
     CDS             complement(22406..23290)
                     /gene="cdd"
                     /locus_tag="IU325_11535"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP5809653.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            complement(23420..24115)
                     /locus_tag="IU325_11540"
     CDS             complement(23420..24115)
                     /locus_tag="IU325_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HAP5809654.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(24112..24510)
                     /locus_tag="IU325_11545"
     CDS             complement(24112..24510)
                     /locus_tag="IU325_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP5809655.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            24749..25696
                     /gene="dusC"
                     /locus_tag="IU325_11550"
     CDS             24749..25696
                     /gene="dusC"
                     /locus_tag="IU325_11550"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HAP5809656.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPIK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMTISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
BASE COUNT         6306 a         6455 c         6753 g         6357 t
ORIGIN      
        1 tctggattgt gatccgggtc atgatgatgc tattgccata atgatggcag cgaaacatcc
       61 ggcaatagat ttattaggca tcaccattgt ggcgggtaat cagacgcttg ataaaacatt
      121 aattaatggc ctgaatgttt gccagaaact ggagattaat gttccggttt atgcggggat
      181 gccacagccc attatgcgtc aacaaatcgt tgccgataat attcacggtg aaaccggact
      241 ggatggcccg gtattcgagc cgctgacccg ccaggcagaa agcactcatg cggtgaaata
      301 tatcatcgat accctgatgg caagcgatgg cgatatcact ctggtgccgg ttggtccgct
      361 ttcaaatatc gcggtggcaa tgcgcatgca gcccgcaatc ctgccgaaaa tccgtgaaat
      421 tgtgctgatg ggcggcgctt acggtactgg caactttacg ccatccgccg agttcaacat
      481 ctttgctgac ccggaagccg cacgcgtggt gttcacctcc ggcgttccgt tggtgatgat
      541 gggcctcgat ctcaccaacc agaccgtttg caccccggac gtgattgccc ggatggaaag
      601 ggcaggcggc cctgccggag agctgttcag cgacatcatg aacttcactc tcaaaaccca
      661 gttcgaaaac tatggcctgg ctggcggccc ggtgcacgac gccacctgca tcggttatct
      721 gatcaaccct gatggcatta aaacccagga tatgtacgtc gaagtggatg ttaacagtgg
      781 cccttgctat gggcgtaccg tctgcgacga gctgggcgtt cttggcaagc cagccaatac
      841 caaagtcggc atcactattg ataccgactg gttctggggc ttagtcgaag agtgcgtgcg
      901 cggctacatc aaaacccatt aagtgttgat aacaagccgg gccggtatcg caagatgcag
      961 gcccggctat ttctgatggg cagaaagccg tcgcccaaaa tgacatgaag agataataac
     1021 tatggatgtc atgagaagtg ttctgggaat ggttgtattg ctggcaattg cgtttttgct
     1081 gtcagtaaac aagaagaaga tcagcctgcg taccgttggc gcggcgttag tgttacaggt
     1141 cgtgattggc ggcattatgc tttggttacc accagggcgt tgggtcgcag aaaaagtcgc
     1201 ttttggcgtg cataaagtga tggcgtacag cgatgcaggt agcgcattta tctttggttc
     1261 tctggtcgga ccgaaaatgg ataccttatt tgatggcgca ggatttatct ttggtttcag
     1321 ggtattaccg gcaattatct tcgttacagc actggtgagt attctctact acatcggtgt
     1381 gatggggatc ttaattcgca ttctcggcgg tatcttccag aaagcattaa atatcagcaa
     1441 gatcgagtca ttcgtcgcgg tcactaccat tttcctcggg caaaacgaaa ttccggcaat
     1501 cgtcaaaccc tttatcgatc ggctgaatcg caatgaatta tttacagcga tttgtagtgg
     1561 catggcctcg attgctggtt cgacaatgat tggttacgct gcactgggcg tgcctgtgga
     1621 atatctgctg gcggcatcgt taatggcgat ccccggcggg atcttgtttg cccgcctgtt
     1681 aagcccggct accgaatctt cgcaggtttc ctttaataac ctctctttca ccgaaactcc
     1741 gccaaaaagc tttatcgaag cagcggcgag cggtgcgatg accggactga aaatcgccgc
     1801 aggtgtggcg acagtggtga tggcatttgt tgcaataatt gcgttgatta acggtattat
     1861 cggcggcgtt ggcggctggt ttggttttga acatgcctcg ctggagtcca ttttaggtta
     1921 cctgttggct ccactggcgt gggtgatggg ggttgactgg agtgatgcga atcttgccgg
     1981 gagtttgatt ggacagaaac tggcgattaa tgaatttgtc gcttacctca atttctcacc
     2041 ttatctgcaa acagctggca ctctggatgc taaaaccgtg gcgattattt ccttcgcgtt
     2101 gtgcggtttc gctaactttg gttctatcgg ggtggtggtg ggggcgtttt ctgcggttgc
     2161 gccacaccgt gcgccggaaa tcgcccagct tggtttacgc gcgctggcgg cggcgacact
     2221 ttctaacctg atgagtgcca ccattgcagg attctttatt ggcttagcgt agtgcgcgat
     2281 ttatgagccg gataagacgc ggtcaagcgt cgaatccggc aagattgtta caccatcggc
     2341 actaatgaaa gcgcgttatc ggcagacagg gtagggttgt ttaagctgcc gctggcgcgt
     2401 gaaattgccg cgcaggccat cgcaaaacgg gcgctgtcac ggaaattgtt tccttccaga
     2461 aagctatata ccaggcccgc cataaaacca tcgtcagcac caaaactgtc tactgtcgta
     2521 tgcgctggtg cggtcagcaa aaattgttct ccatcctttt cgctgcaata aactgactca
     2581 tcgggcaaat aaacaaacag ttgctgaacg ccttgctgat gcaacgcatt cactgcggta
     2641 ttacggtcag catcgctggt gatcgcctgt ccccataaaa tttccagctc cggtaaagtg
     2701 ggtttcaggg tgtgaatatg cgccagccag tgtttgattt tgcccgcttt gaattctgaa
     2761 acggtatcga caaacaccgg gatttcatca gcgagggtaa agacccactc cagcgcctcg
     2821 gctgtcaggt tgcaatcagc cagtaccacg cccgcatgac gaagtaaatc gcgcgacccg
     2881 ttcagtagtt gcggtgtcaa ctgttccagc agatgggtat cgttaatcgc cagcacggtt
     2941 tgatcgtctc gattggcaat tgccagatac gtcgatgtgc tttgaccatg taaacgaacg
     3001 cagccggaga cattcacgcc ggcgcggcgc gtttcttcca ggagcatttc gccataaaag
     3061 tcatcgccaa tcactgaaag caaatggacg tcgcggccta acagcgccag attgtgggcg
     3121 atattgcgtc caacgccgcc tgccgagcaa tgaattgtac cgggatgaga agccgcttgc
     3181 gggtaacgga tatccgccat cccgcgaata tccatattga ttgtccccac cactacgcag
     3241 tattcctgct cggtgagaat gtaacctttg cctttaatcc gacctttgcg cattaaatcc
     3301 ataatatgcg ccgcaacgcg cgaacggctg atttgcagca tatcagcaat ttcgttctgc
     3361 tgaatcagcg ggttacgccg taaaattgca aggatctcct tctcccggtt gttcatcttc
     3421 aggctaccgc tttttcagtt tgttgcgctt tcagccaggc gatctcttct gcccagatat
     3481 ccgggttgat ggtttcaagg atcagcggaa tgccgtcgaa acggtcgtcc tgcatgatcc
     3541 agcggaacgc atcatgaccg atattgcctt caccgaggct atgatggcgg tcaacgcggc
     3601 tgccaaaggt gcttttcgca tcgttaaggt gcatcccgcg cagatactta aagccgacaa
     3661 tacgggcaaa atccgcgaac gttttctcgc attcggctgg agtacgcaaa tcatacccgg
     3721 cagcaaaagc atggcaggta tcaatgcaga cgccgacgcg ggatttatct tccacgccgt
     3781 cgataatcgc cgcgagatgt tcgaatttaa accctaagtt actgccctga ccggcggtgt
     3841 tttcaatcac cgctgtcaca ccttgagttt tatccagcgc aatgttgatg gattcggcaa
     3901 tacgcgcaag gcaatcctct tctgaaatct gcatcagatg gctgccaggg tggaagttga
     3961 gcaaagaaag ccccagctgt tcgcaacgct gcatttcatc tataaacgca tcgcgcgatt
     4021 tttccagagc ttcagcgacc ggatgtccga ggttaatcag ataactgtcg tggggaagaa
     4081 tttgcgccga tgtgtagtga tatttttcac aggcggcttt gaattcatcg atggtttgcg
     4141 tcgtgagcgg tgcggcacgc cactgacgtt ggtttttggt gaacaaggca aacgcggttg
     4201 cgtcgatttc ggcggcgcga attgcggcat ttgccagacc gccagcagca ctaacgtgcg
     4261 ctccaatgta tttcatgcga ggactcctgt taaacccgct ggaggaaaac ggtaatgata
     4321 gcgggttaac aggagcaaga tgtagtggtt tatgcgatga cgctttgaat cacatagtta
     4381 atcgcaccac caccaacaat cagccaggca aacagtacca gtgccatcag cagaggtttc
     4441 gccccggctt ttttcagcgc gctgacgtga gtggtcagac ccagcgccgc catcgccatt
     4501 gccagcagga aggtatccag cgttaccagc atgttcacca cgctctgcgg taacaggtgg
     4561 aacgagttaa agatggcaac tacgatgaac aagatggcaa accacggaat agtgattttg
     4621 cttttctcgc cgctgttcgc cccagacagc tgtttaacac gcgccgccag caggatgagg
     4681 aacggagcca gcatcatcac gcgcagcatt ttggaaataa ctgctgcgtt ttctgcatcc
     4741 gggctgatgg catgacctgc cgccaccacc tgcgccactt cgtgcacagt agaaccaatg
     4801 tagataccga aagtttccgg actaaaccat tgagacatca gcggatatat cgccgggtag
     4861 aggaaaatcg cgacggtccc gaagataaca acggttgcaa cagccacggt tactttactg
     4921 gcttccgctt tcactaccgg ctcagtcgcc agtaccgcgg cagcaccaca aatactgcta
     4981 ccggcaccga tcaaccagct ggtgtgctta tccagaccaa acactttctg ccccaggaag
     5041 caagccagca ggaaggtact ggacagcgtc agcacgtcaa tgatgatccc actgataccg
     5101 acatcggcaa tttgcgagaa cgtcagacgg aagccataaa gaatgatacc cagacgtaat
     5161 aaatattgct tggcaaacag cacaccaccg tcacagcttt tccagatgtg cggatagatg
     5221 gtgttgccta aaaccatccc caacaaaatt gcgagggtga gggcactaaa cccggcaccc
     5281 gcaaccgcgg gaatggaacc accccacagg gcgaccccgg tgataactgc actcagggct
     5341 aaccccggaa taaaatgcca cagtgtacga tgttgtttct gtaaggtgat attcgtcata
     5401 accctctcct ttaaccgggc ataaggttac gactaactgg tttaaaaata aaattgatta
     5461 tatatttata attaatcttt ataagtggta agcgactatg cacatcaccc tccggcagtt
     5521 ggaagttttt gcagaagtat tgaaaagtgg atcaaccacc caggcgtcgg tgatgctggc
     5581 gttgtcgcaa tcagcagtga gcgcagcctt gaccgacctg gaagggcagc ttggcgttca
     5641 actgtttgat cgcgtgggga aaagactggt agttaatgaa cacgggcgac tgctctatcc
     5701 gcgcgcgttg gcactgcttg aacaggcgat cgaaatcgaa caactgtttc gcgaagacaa
     5761 cggtgcgatt cgtatctatg ccagtagtac catcggtaac tacattctgc ctgcagttat
     5821 cgcccgttat cgccatgatt atccgcagtt gccgattgaa cttagcgttg ggaacagcca
     5881 ggacgtgatg caagcggtgc tggatttccg cgttgatatt ggctttattg aaggaccgtg
     5941 ccacagcact gaaatcatct cagaaccgtg gctggaagac gagctggtgg ttttcgccgc
     6001 gccgacttca ccattggctc gtggacctgt caccttagaa cagctggccg ctgcgccgtg
     6061 gatcctgcgt gaacgcggct ccggcacgcg ggagattgtc gattatctgt tgctgtcaca
     6121 tttaccgaag tttgagatgg cgatggaatt aggtaactcc gaggcaatca aacatgcggt
     6181 gcgtcatggg ttgggaatta gttgcctgtc gcggcgggtg attgaagatc aattgcaggc
     6241 aggcacatta agtgaagttg cggtccctct gccgcgcctg atgcgtacgt tgtggcgtat
     6301 acatcatcgg caaaaacacc tttccaacgc gctacggcgc tttctggact attgcgatcc
     6361 cgcgaatgta ccgcgttaag ttgctgtaca agaacatgct ggtgctgtgt cgatttcgtg
     6421 acgcagcgcc ctcagcatgc attcgccaga aaagagattg gctgctttac ttataatccc
     6481 tgggcgatca tgaaggtgtc ttataaccgt gtatttctgc cggaaggatt gccaatcgtc
     6541 tgctacaatc gcgcctcatt tttaagatgg atagcatttt tgtatgggtt ccgaaactaa
     6601 aactacagaa gcgccgggct tacgccgtga attaaaggcg cgtcacctga cgatgattgc
     6661 cattggcggt tccatcggta caggtctttt tgttgcctct ggcgcaacga tttctcaggc
     6721 aggtccgggc ggggcattgc tctcgtatat gttgattggc ctgatggttt acttcctgat
     6781 gaccagtctc ggtgaactgg ctgcatatat gccggtttcc ggttcgtttg ccacttatgg
     6841 tcagaactat gttgaagaag gctttggctt cgcgctgggc tggaactact ggtacaactg
     6901 ggcggtgact atcgccgttg acctggttgc agctcagctg gtcatgagct ggtggttccc
     6961 ggatacaccg ggctggatct ggagtgcgct gttcctcggc gttatcttcc tgctgaacta
     7021 catctcagtt cgcggctttg gtgaagcgga atactggttc tcactgatca aagtcacgac
     7081 agttattgtc tttatcatcg ttggcgtgct gatgattatc ggtatcttca aaggcgcgca
     7141 acctgcgggc tggagcaact ggacaatcgg cgaagcgccg tttgctggtg gttttgcggc
     7201 gatgatcggc gtagctatga ttgtcggctt ctctttccag ggaaccgagc tgatcggtat
     7261 tgctgcaggc gagtccgaag atccggcgaa aaacattcca cgcgcggtac gtcaggtgtt
     7321 ctggcgaatc ctgttgttct atgtgttcgc gatcctgatt atcagcctga ttattccgta
     7381 caccgatccg agcctgctgc gtaacgatgt taaagacatc agcgtcagtc cgttcaccct
     7441 ggtattccag cacgcgggtc tgctctctgc ggcggcggtg atgaacgcag ttattctgac
     7501 ggcggtgctg tcagcgggta actccggtat gtatgcgtct actcgtatgc tgtacacctt
     7561 ggcgtgtgac ggtaaagcgc cgcgcatttt cgctaaactg tcgcgtggtg gcgtgccgcg
     7621 taatgcgctg tatgcgacga cggtgattgc cggtctgtgc ttcctgacct ccatgtttgg
     7681 caaccagacg gtatacctgt ggctgctgaa cacctccggg atgaccggtt ttatcgcctg
     7741 gctggggatt gccattagcc actatcgctt ccgtcgcggt tacgtattgc agggacacga
     7801 cattaacgat ctgccgtacc gttcaggttt cttcccattg gggccgatct tcgcattcat
     7861 tctgtgtctg attatcactt tgggccagaa ctacgaagcg ttccttaaag ataccattga
     7921 ctggggcggc gtagcggcaa cgtatattgg tatcccgctg ttcctgatta tttggttcgg
     7981 ctacaagctg attaaaggaa ctcacttcgt acgttacagc gaaatgaagt tcccgcagaa
     8041 cgataagaaa taagtttcct cccttccttg ctaagccctc tcaaccgaga gggctttttc
     8101 aattccattt ccctgacaaa tcatgcggat ataaaattta acatttggat tgataattgt
     8161 tatcgtttgc attatcgtta cgccgcaatc aaaaaaggct gacaaatcag aggctgttcc
     8221 ggctttctgg gatgatcacc tgcatacaaa taagtccacc gcgatgctgc cgtacgcaag
     8281 gggacatgaa gaagatgtga gcgataaccc attttatttt cgtagttacc tcatggagat
     8341 atggaatgtt taggttgaac cctttcgtac gggtcgggct gtgtttgtcc gctatttctt
     8401 gtgcatggcc tgtgttagcg gtcgatgatg atggcgaaac gatggttgtc actgcatctt
     8461 ccgttgagca aaaccttaaa gatgcacccg ccagtatcag cgtcattacc caggaagacc
     8521 tgcagcgaaa accggtacag aatctgaagg atgtcctcaa agaagtgcct ggcgtacaac
     8581 tgacgaacga aggggataac cgtaagggcg ttagtattcg tggtctggac agcagctaca
     8641 ccctgattct cgtcgacggt aaacgcgtta actcccgcaa tgccgtcttc cgccacaatg
     8701 atttcgatct gaactggatc ccggtcgatt ccatcgaacg tattgaagtg gtccgtggcc
     8761 cgatgtcgtc gctgtacggt tccgatgcgc tcggcggtgt agtgaatatc atcaccaaaa
     8821 aaatcggtca gaaatggtcg ggtaccgtta ccgtcgatac caccattcag gaacatcgcg
     8881 atcgcggtga tacctataac ggtcagttct ttaccagcgg accattaatt gacggcgtgc
     8941 tgggaatgaa agcttacggc agcctggcaa aacgtgaaaa ggatgacccg caaaactcaa
     9001 cgaccaccga taccggagaa acgccgcgta ttgaaggatt ctccagccgc gacggcaatg
     9061 tggaatttgc ctggacaccg aatcaaaatc acgattttac tgccggatac ggtttcgacc
     9121 gtcaggatcg tgattccgac tcgctggaca aaaaccgcct ggaacgccag aactactccg
     9181 tcagccataa tgggcgttgg gattacggca ccagcgaact gaaatactac ggtgagaaag
     9241 tcgagaacaa aaaccctggc aacagcagcc cgataacttc cgaaagcaat acggtcgacg
     9301 gcaaatacac gttgccgctg acggcgatta atcagtttct cacggttggc ggtgaatggc
     9361 gtcacgacaa acttagcgat gcggtgaacc tgaccggggg aaccagttcc aaaacgtctg
     9421 ccagccagta cgcgctgttt gtggaagatg aatggcggat cttcgagccg ctggcgctga
     9481 cgaccggcgt acgtatggac gatcatgaaa cctacggtga acactggagt ccgcgtgcct
     9541 acctggttta taacgccacc gacaccgtaa cggtgaaagg gggctgggcg acggcattta
     9601 aagcaccttc tctgttgcaa cttagccctg actggacgag caattcctgc cgtggcgcat
     9661 gtaagattgt gggtagcccg gatctgaaac cagaaaccag cgaaagttgg gaactggggc
     9721 tttactacat gggcgaagac ggctggctgg aaggggtaga gtccagcgtt accgttttcc
     9781 gtaacgatgt gaaagatcgc atcagcatca gccgtacgtc tgacgtcaat gctgcaccgg
     9841 gctaccaaaa ctttgttggt tttacagcga acggtgtgcc ggtatttagc tactacaacg
     9901 ttaacaaagc tcgtattcag ggcgtggaaa ccgaactgaa aattccgttc aacgatgaat
     9961 ggaaactgtc gatcaactac acctacaacg atggtcgtga tgtcagcaac ggcgaaaaca
    10021 aaccgctatc cgatctgccg ttccatactg ctaacggtac gctggactgg aaaccgctgg
    10081 cgctggaaga ctggtcattc tatgtttctg gtcactatac cgggcagaaa cgagccgaca
    10141 gcgcgacggc taaaacaccg ggcggttata ccatctggaa taccggcgcg gcctggcagg
    10201 tgactaaaga cgtcaaactg cgcgcaggcg tgctgaacct tggcgacaag gatctcagtc
    10261 gtgacgacta cagctataac gaagacggac gtcgttactt tatggcagtg gattatcgct
    10321 tctgatgaga agatgcccgg cgaaccgggc ggactttcac ttcagtaaat attgcgcatg
    10381 gaagcgcaaa tgatcctcta taaaagaggc gatgaagtag taactgtgat catatcccgg
    10441 ctgaatacgc agcgtcatcg gccacgcttt ctggcgcgcg gcttctgcca gcactgcggg
    10501 ttgcagctgg tcggcaagaa actgatcatt atcaccctga tcgataagcg tcgggatcgc
    10561 atcctgcgcg ttactggcat acatcagcgc gcaactgtcc cattccagcc atgcattttt
    10621 gtcctcacct aaataggtgc taaacgcttt gattccccac gggacgctgc acggattcac
    10681 aattggcgca aaggccgaaa cgctagtgta tttacccgga tttttcagcg ccataattag
    10741 cgcaccgtga ccacccattg aatgaccgct aatagcgcag cggtcgctga cattaaattg
    10801 cgactgaatc agcgccggta attcatcgcg cagataatca tacatccggt aatgcgtcgc
    10861 ccaaggcggt tgcgtggcat taagataaaa gcctgcgccc tggcctaaat cgtagccatc
    10921 gtcgttggca acctgttcgc cgcgcgggct ggtgtctggc attaccagta caatccccag
    10981 ttctgccgcc acgcgctggg cacccgcctt ggtggtgaag ttctcgtcat tgcaggttaa
    11041 tccggaaagc cagtacagca ctggaggcgg ggtgtgatca cgaggtggtg ggagaaagat
    11101 actgaacgtc atcgggcagt ttaaggtact ggagtcgtgt cgccagcgtt gctgccagcc
    11161 ttcaaaacag cggtgctctt cgagcatttc catgcgtggc tccttgttgt gttgtttgca
    11221 atttcttcat cataccgttt attcaatgtg ctgtgagtaa ctttcacttc cgtatttgca
    11281 taacgatgtt ttaacatctg ctgatgaaag gcagcggcaa ttacaataat tatcgctgtg
    11341 aatactggat tatgtgcgcc gcctcacgca caataatcag gctgtaaatc agcttaataa
    11401 ctttgccccc acgcagggcg gaggcgtcac acctgcagga gaaatcataa atgccatcac
    11461 tcagtaaaga agcggccctg gttcatgaag cgttagttgc gcgaggactg gaaacaccgc
    11521 tgcgcccgcc cgtgcatgaa atggataacg aaacgcgcaa aagccttatt gctggtcata
    11581 tgaccgaaat catgcagctg ctgaatctcg acctggctga tgacagtttg atggaaacgc
    11641 cgcatcgcat cgctaaaatg tatgtcgatg aaattttctc cggtctggat tacgccaatt
    11701 tcccgaaaat caccctcatt gaaaacaaaa tgaaggtcga tgaaatggtc accgtgcgcg
    11761 atatcaccct gaccagcacc tgtgaacacc attttgttac cattgatggc aaagcgacgg
    11821 tggcttatat cccgaaagat tcggtgatcg gtctgtcaaa aattaaccgt attgtgcagt
    11881 tctttgccca gcgtccgcag gtgcaggaac gtctgaccca gcaaattctc attgcgctac
    11941 aaacgctgct gggcaccaat aacgtggctg tctctatcga cgcggtgcat tactgcgtga
    12001 aagcgcgtgg catccgcgac gcaaccagtg ccacaacaac gacctctctt ggtggattgt
    12061 tcaaatccag tcagaatacg cgccacgaat ttctgcgcgc tgtgcgtcat cacaactgat
    12121 taaaaggcag gaaccatgga gcgcaacgtc acgctcgatt ttgttcgcgg cgtcgccatt
    12181 ctgggggtcc tgctattaaa catcagcgcc tttgggctac caaaggcggc ttatctcaat
    12241 cccgcctggt acggcgctat tacgccgcag gatgcatgga cctgggcatt tctcgatctc
    12301 atcggccagg tgaaattcct cacgcttttt gcgctgctgt ttggtgctgg cctgcaaatg
    12361 ttgctgcccc gtggcagacg ctggatccag tcgcggttaa cgctgttagt cttgctgggc
    12421 tttattcacg gtttattgtt ctgggacggc gatattctgc tggcttacgg gctggtgggc
    12481 ttaatctgct ggcggctggt gcgcgatgcg ccatcagtaa aaagcctgtt taatacaggc
    12541 gtcatgcttt atctggtggg gcttggcgtt ttgatgttat tggggctgat ttccgacagc
    12601 cagaccagcc gcgcctggac gccggatgca tcggctattt tgtatgaaaa atactggaag
    12661 cttcacggcg gcgttgaagc gatcagtaat cgtgccgacg gtgttggcaa cagtttactg
    12721 gcactgggcg cacagtatgg ctggcaactg gcagggatga tgctcattgg tgcggcgctg
    12781 atgcgcagcg gctggctgaa agggcagttc agcttacgtc actatcgtcg tactggtttt
    12841 gtgctggtgg cgattggggt gaccattaac cttcctgcca tcgccctgca atggcagctg
    12901 gactgggcgt atcgctggtg tgccttctta cttcaaatgc cgcgggaact gagtgcgccg
    12961 tttcaggcga ttggctatgc gtcgctgttt tatggtttct ggccgcaatt aagccgcttt
    13021 aagctggtgc ttgcgatcgc ctgcgtcgga cggatggcgc tgaccaacta tctattgcaa
    13081 acgctgattt gtaccacgct tttttaccac ctcggtttgt ttatgcattt tgaccgcctg
    13141 gagctgctgg cgtttgttat tccggtatgg ctggcgaata tcctcttctc tgttatctgg
    13201 ctgcgttact tccgccaggg gccggtggaa tggctctggc gtcagttaac tttgcgtgct
    13261 gccggaccgg caatatctaa aacatcaaga taacgatctg gatcacattc gttaacaaaa
    13321 cggctgtaac cgtttccatt gctgtgactc gattcacgaa gtcctgtatt cagtgctgac
    13381 aaaatagccg ccagcaagca gtcatttact gcaatctcat aacaggtagt gaatatgatc
    13441 accattcgtg atgtagcgcg tcaggctggc gtctctgtgg caacggtttc ccgagtgctc
    13501 aataacagta cgctggtcag tgccgacacg cgtgaagcag taatgaaagc cgttagtgag
    13561 ctggattatc gaccaaatgc caatgcccag gcgctggcaa ctcaggttag cgacaccatt
    13621 ggcgtggtgg tgatggacgt ttctgatgcg tttttcggcg cgctggtaaa agcggtggat
    13681 ctggtcgccc agcagcatca gaaatacgtg ctaatcggca atagctatca tgaagcggaa
    13741 aaagagcgtc acgccattga ggtgttaatt cgccagcgtt gtaatgcatt gattgttcac
    13801 tcaaaagcat tgagtgacga tgaactggca caatttatgg ataacattcc cggtatggtg
    13861 ttaatcaacc gcgttgtgcc ggggtacgcc catcgttgcg tttgcctgga taatctcagc
    13921 ggtgcccgaa tggcgacgcg catgttgctg aataacggtc atcaacgtat tggttatctt
    13981 tcttccagtc acggcattga agatgacgcc atgcgtaaag caggctggat gagtgcgttg
    14041 aaagagcagg atattattcc gccggaaagc tggattggca ctggcacgcc ggacatgccg
    14101 ggcggtgagg cggcgatggt tgaactgctg gggcgcaatc tacaacttac cgctgtattt
    14161 gcttataacg acaatatggc cgctggcgca ctgacagcat taaaagataa tggcattgcg
    14221 attccgttac atctctcaat catcggtttc gatgatattc ccatcgcccg ttacaccgac
    14281 ccgcaattaa cgactgtgcg ttatcccatt gcttcaatgg cgaaattagc caccgaactg
    14341 gccttgcagg gggcagcagg caatattgat ccgcgtgcca gccactgttt tatgccgacg
    14401 ttagtgcgtc gccattctgt cgcaacgcgc cagaatgcgg cggcgatcac taactcaaca
    14461 aatcaggcga tgtaaccgct ttcaatctgt gagtgatttc acagtatctt aacaatgtga
    14521 tagctatgat tgcaccgttt taacgttgta acccgtatgt aacagtgaat aatcactttc
    14581 accgaggtaa tagcgtcata acaacaatta aagccgtttt ctggagcgtt accgggcatg
    14641 gaagaacgaa ttttaaaaag tgagcttcgg cgttcagtaa cacttcatta actctactgc
    14701 cccgccgagc atttatctca agcactaccc tgcataagaa aaaccggaga taccatgaat
    14761 aagaaggtgt taaccctgtc tgctgtgatg gccagcatgt tattcggtgc cgctgcacac
    14821 gctgctgata ctcgcattgg tgtaacaatc tataagtacg acgataactt tatgtctgta
    14881 gtgcgcaagg ctattgagca agatgcgaaa gccgcgccag atgttcagct gctgatgaat
    14941 gattctcaga atgaccagtc caagcagaac gatcagatcg acgtattgct ggcgaaaggg
    15001 gtgaaggcac tggcaatcaa cctggttgac ccggcagctg cgggtacggt gattgagaaa
    15061 gcgcgtgggc aaaacgtgcc ggtggttttc ttcaacaaag aaccgtctcg taaggcgctg
    15121 gatagctacg acaaagccta ctacgttggc actgactcca aagagtccgg cattattcag
    15181 ggcgatttga ttgctaaaca ctgggcggcg aatcagggtt gggatctgaa caaagacggt
    15241 cagattcagt tcgtactgct gaaaggtgag ccgggccatc cggatgcaga agcacgtacc
    15301 acttacgtga ttaaagaatt gaacgacaaa ggcatcaaaa ctgaacagtt acagttagat
    15361 accgctatgt gggataccgc tcaggcgaaa gataagatgg acgcctggct gtctggcccg
    15421 aacgccaaca aaatcgaagt ggttatcgcc aacaacgatg cgatggcaat gggcgcggta
    15481 gaagctctga aagcacacaa caagtccagc attccggtgt ttggcgtcga tgcgctgcca
    15541 gaagcgctgg cgctggtgaa atccggtgca ctggcgggca ccgtactgaa cgatgctaac
    15601 aaccaggcta aagcgacctt tgatttggcg aaaaacctgg ccgatggcaa aggtgcggct
    15661 gatggcacca actggaaaat cgacaacaaa gtggtccgcg taccttatgt tggcgtagat
    15721 aaagacaacc tggctgaatt cagcaagaaa taagactgat tgggcgcagt cgtttactgc
    15781 gcccttattg tatggccaac aaggtaaaat tatatggtca gctcaacgac tccgtcttcc
    15841 ggggaatact tgttggaaat gagcggtatc aacaagtctt ttcctggtgt taaggcactt
    15901 gataacgtta atttaaaagt ccggccacat tctatccatg cattaatggg ggaaaacggc
    15961 gcaggaaaat caacattatt aaaatgcctg tttggtattt atcaaaaaga ctccggcacc
    16021 attttattcc agggtaaaga gatcgatttc cattctgcca aagaagcgct ggaaaatggt
    16081 atttcgatgg tacaccagga gttaaacctg gtattacaac gttcggtgat ggataacatg
    16141 tggctggggc gatatcccac caaaggcatg tttgtcgatc aggacaaaat gtaccgcgaa
    16201 accaaagcga tttttgatga actggatatt gatatcgatc cgcgtgcgcg cgtcggcaca
    16261 ttatccgttt cgcaaatgca gatgatcgaa atcgccaaag cgttttccta taacgcgaaa
    16321 attgtgatta tggatgaacc gacttcttcg ttaaccgaaa aagaggtcaa tcatctgttc
    16381 actattattc gtaaattaaa agagcgcggc tgcggtattg tttatatctc acataaaatg
    16441 gaagaaatct tccagttatg tgatgaagtt accgtattgc gcgacggtca gtggatcgcc
    16501 actgaaccgc tggcaggact gacgatggac aagatcatcg ccatgatggt tgggcgttct
    16561 cttaaccagc gtttccctga caaagaaaac aagccgggcg aagtgattct tgaagtgcgt
    16621 aacctgacgt cgctgcgcca gccgtcgatt cgcgatgtct cgtttgatct gcataaaggg
    16681 gagatcctcg gtattgccgg tctggtgggg gcgaaacgta ccgatattgt tgagacgtta
    16741 tttggtattc gcgagaaatc ggctggcacc attacgttgc acggcaaaaa gatcaataac
    16801 cataatgcca acgaagccat aaaccacgga tttgcactgg taactgaaga gcgccgctca
    16861 acgggaattt atgcctatct ggatatcggt tttaactcgt taatttccaa tattcgcaac
    16921 tacaaaaata aagttggttt actggataac tcgcggatga aaagtgatac ccagtgggtg
    16981 attgattcga tgcgggtaaa aacaccgggg catcggacgc aaattggttc gctctccggt
    17041 ggtaatcagc aaaaggtgat tattggtcgc tggctgttaa cgcaaccaga aatattaatg
    17101 ctcgatgaac cgacgcgcgg tattgatgtc ggggcgaagt ttgagatcta tcagttaatt
    17161 gccgagctgg cgaagaaagg caaggggatt attattatct cctccgaaat gccagagttg
    17221 ttagggataa cagaccgtat tctggtcatg agtaatggtc tcgtttccgg aattgtcgat
    17281 acaaaaacaa caacgcaaaa cgaaattctg cgtcttgcgt ctttgcacct ttaagattag
    17341 gggcttccca tgagtgcgtt aaataagaaa agttttctta cttacctgaa agagggcggt
    17401 atttacgtcg ttcttttagt tttgctggca attattattt tccaggatcc tacattttta
    17461 agtctgttga acttaagtaa tattctcacc cagtcatcgg tgcgtattat tatcgcgctt
    17521 ggtgtggcag ggttaattgt cacccagggg accgaccttt ctgcggggcg tcaagtaggg
    17581 ctggcggcag tggtggcagc gacattattg cagtccatgg ataacgccaa caaagtgttc
    17641 ccggaaatgg cgacgatgcc gattgcgctg gttattctga ttgtctgtgc cattggtgcg
    17701 gtgatcggtt tgatcaacgg tctgattatc gcctatctca acgtgacgcc gttcattacc
    17761 acgctcggca cgatgatcat cgtctatggc atcaactcgc tctattacga ctttgtcggg
    17821 gcgtcgccga tttctggttt cgacagtggc ttctctacct ttgctcaggg ctttgtcgcg
    17881 ctggggagtt tccgtctctc ttacatcacc ttctacgcgc tgattgcggt ggcgttcgtc
    17941 tgggtgttgt ggaacaaaac ccgcttcggc aagaacattt ttgctatcgg cggtaacccg
    18001 gaagcggcaa aagtttctgg tgtcaacgtc ggcctgaacc tgctgatgat ctacgcgttg
    18061 tctggtgtgt tctatgcctt cggcgggatg ttagaagccg gacgtatcgg ctctgccacc
    18121 aacaacctcg gctttatgta tgaactggat gctatcgcgg cgtgcgtggt gggcggcgta
    18181 tcgttcagcg gcggtgtggg gacggtgatt ggcgtggtga ccggggtaat tatttttacc
    18241 gtcatcaact atggcctgac gtacatcggt gtaaacccgt actggcagta catcatcaaa
    18301 ggggcgatta ttatcttcgc cgtggcgctg gattcactga aatacgcgcg taagaagtga
    18361 tgtgattgtg ggtattgccg gatgcgtcgc atccggcaat cggtgccaaa tgccggatgc
    18421 gtcgctggtt ttacaatgtt acagagtgtt ctttctctcc cttcttaaac ttcacttccc
    18481 caagctcaat acaacccacc gggcagacgt gaccacaaag cagacaaccc acgcatttct
    18541 cggtattaca atgcggcgtg cgtgttttct cgctccattc catcgcctgg tgaccgccgt
    18601 cgtaacagga aatataacag cgtccacagc caacacattt atcgagattg atacggggat
    18661 agacaatata actgcggtct aaatcttccg ccggaacaat attgttattc gccagaccta
    18721 ccatctcctg caacgaatca aatccctgat cggcgagata atgcgacaac ccgctcgcca
    18781 tatcttccac tatccgatac ccgtactgca taatgccggt ggtcacctgt aacgttgctg
    18841 cgccgagcaa taaaaactcc gccgcatcct cccaggtttc aatgccgccg ataccgctga
    18901 tcgggaaatc gcgcagctct ggatgggtac gtatttgctg aatgaagcgc agggcgatcg
    18961 gttttaccgc tttaccggaa tatccggaaa tactcgattt accgttaacg atcggcatac
    19021 cgattttctg attaagatcg atattggtga tggatttaac ggtgttaatc gccgcaatgc
    19081 catctgcgcc gccgcgcttc gccgccagcg ccacttcgca catatcaccg atattcggcg
    19141 tcatcttcgc cagcattggc agcgtggaac cacgttttac tgcccgacaa tatttttcta
    19201 ccagctccgg gctttgcccg acatcgctac ccatcgcatg agaagtcatt tgcggacagg
    19261 agaagttaca ctcgatcata tccgcgccag cttcttgcac cagacgcgcc agctcttccc
    19321 attgctgctc attttcgccc atgattgaag cgatcaatac tttgtccggg taatcttctt
    19381 tcagccgacg cagggcggcc agattatctt ccaacggatg ttcagcaatc tgctccatat
    19441 ttttgaagcc gataaaaccg gtatcttctt tcaccagatg atcaaaacgc ggcgagactt
    19501 cgttggcgat aaaaaagccg atcgttttaa acaccacgcc gccccagcca gtgtcgtagg
    19561 ctttggcaca catctcatag cagttgccta ccggcgaaga agagaggcag aacgggttgg
    19621 gaaacttcac gccgcaaaaa gtaatcgaaa gatctttcgt taacatgagc aagctccctc
    19681 taaatagtga tgaatcgcct cggcggcttc tttcccggtt ttcacggcat agaccacggt
    19741 tttgtccccc tcaacaatat cgccagcagc aaagacttgc ggattgcggg tctggtaatt
    19801 ttgtgtattg atggtgttac gctgcggctc taactccgca aaggcatcca gtctggcatg
    19861 ctgaccgacg gcgagaataa ttttatctgc cgccatcgtc agttcgcccg gtaaccgtac
    19921 atgcttaaac gtgactttat tgccttccac ggctactggc gtgaatccat cgatgatcga
    19981 aacgcccagc tcccgagcgc tggcaaactc tttttcgctt gccggaaact catctaactc
    20041 ttcacgcgct acgcaagtta ccgcctgaca gccgagaact ttcagcgtgc tggctacgtc
    20101 catcgcgaca tcaccgccgc cgataattaa tgcgctttgt ggaatgctga tatcgccttg
    20161 cgcctgtcgt gcacgttgca agaagtcgac ggcaatctca acgtcgctat gctcaaacag
    20221 tggtagcccc gaaccgctcg ataaccccac ggtgaccagt accgcgcggt tttccgcttt
    20281 cagctgatcc agggtaaatg tgttaccgat ttcgttattg cacttaatgg tcacgcccat
    20341 tttttcaata cgggcgattt ctgcatccag tactgattgc ggtaaccgga attgcggaat
    20401 gccgttacgc aaccagccac cgggttgtgc ttctttctca tagatcgtga cgtcataacc
    20461 ctggtttgtc agcgtcacac tggcctgcaa tccggcagga cctgcgccaa taatggcgac
    20521 tttgccgagc gttttagtac cgggctgata aatttccatc ccagtttgtt gttcaaaatc
    20581 agtaacaaaa cgctgtaagc gaccgatatc aatgggagta tcaacaccgg cacgggtaca
    20641 accgctttgg cataatttct ccgtcgggca cactctggca caaacggcac cgagggcatt
    20701 attttcgcga attgtctcgg cagcgccttt aaaattacga aagtagattg agcgaataaa
    20761 tttccccgga tcggtctgtg ccgggcaagc ctgactacag ggggcgtcat ggcataataa
    20821 acagcgagag gcttctttaa tcgccagtaa cggtgtaaaa gccggagtga gttcatccag
    20881 ataattttgt tgcggcatgt gatcttcctt aatgctatga gtaattaagg cgaacgctaa
    20941 atatactcgt catacttcaa gttgcatgtg ctgcgtctgc gggttattcg gcccatccat
    21001 gggcctcacc ccttatgggg ccgctgcagg cagcgttcaa atccgctcca tgcggatttg
    21061 tcgctcaccc cagtcactta cttatgtaag ctcctgggga ctctctcact tgtcgccttc
    21121 ctgcaactcg aattatttag agtataaata ttgcgtgctg atattaaggc tatgcgcgtg
    21181 gggaaattta aactgtgaaa ggattcacaa atagctgtgg ttcagaatgt tatatccgca
    21241 gcttattgag tgaagtaata attattctca ataattaaca gaaaagagaa aatattaacg
    21301 atagaaaata agataaacaa tcatgccgat agataaaatt ccgaagccga tatatgccac
    21361 catataagcg aagaagagtc cgggattttt tgctcgccag acatacaccg gttccggtgc
    21421 atttttaacc cgtttatcaa tcgcgcctgc gcgccagcct ttccatgctt cgcggaaaca
    21481 aaatatcgga atgaggaaaa aaacggcaac gacaaaaaaa tgctgtacat ccataactac
    21541 tttccttgtt tcttttgcaa ttcaagtaac tgttcgggcg tgacggcggg atacccctgc
    21601 gcatcttccg gcacctggtg catagccgga atagggacca gcggccctaa aaaacgcggt
    21661 tcacgtttaa aaatataaag gtcagccagc gcaccgaaac gggcggcaaa ttcgcgaata
    21721 cgtactgaca gcatatcctt cggtgacggt acggcataac actgagcctg aatccccata
    21781 tgcagcgcaa taaataatgc ccgctcacag tggaaacgtt gggtgataat aatgaaatca
    21841 ttagtatcga aaactttgcg tgtacgcacg atggaatcca gcgtacgaaa gcctgcgtaa
    21901 tcgagaacaa tatctgatgg gtcgacacca gcagcgatta aatctttgcg catggtcatc
    21961 ggctcattat aactttgcaa tgcgttatcg ccgctcagta atagataatt taccttaccg
    22021 ctgttatagg cattaatcgc tccttgaatg cggtagcgat aatactgatt aattacgcca
    22081 gtacgataat attttgctgt gccgagcacc acaccgacct ggcggtaggg gagatcctgc
    22141 aattcgtcgt agatataagg cgcggttttc cagctcatcc agcgatcgag gccgagcaca
    22201 gtcaacagca gcaagccgat caggactaac aggctgagga acacgcgctt taacatgcag
    22261 gagactcgat ctaaggaaaa tttccatcag gctactttac ccgctggttt tgcgcaagaa
    22321 acggcaaact gtttgccggg attttcgaca ccaggagagg gctggcaagc cggacaaggc
    22381 gttcacgccg catccggcac caggcttaag cgagaagcac gcggtcgata ttgtgacagc
    22441 cgagagcttt cagcgttgcg gaggtggcat cccactgaat caacggcgca tcggcttttt
    22501 ctgccagaac cgcgcgctgg atatccgggt aatcataacc cttgagattc aacagaatta
    22561 acgccccctg caacggtggc agagtcgggt tgaatgcggc gttttcagcg tagctgccgc
    22621 tgaaaatacg accgtcttta cattccagcg cgacaccgct tggcgactta ctgtaaggca
    22681 tgtgcgaacg gtttgccgcc gcaatcgccg cctgagaaag cgcatcaccc gtcagcgcat
    22741 agccgtgatc ctgctcgtcc atcagcagcg ttttaatctc cagatctttc ggcccaaagg
    22801 catccggtag atagtcacgc agcgcgtgtg cctcgcgtcc cggaagatga atacgcagat
    22861 ccagaccgct gttcagttca ttcataaact gacggcagtg accacaaggc gtgtagttaa
    22921 cggtgatcgc tgcaagcgct ttttcaccac tcaaccaggc gtggctgatc gcgctttgtt
    22981 cggcatgaac ggtttgctgc attgtcgcac caataaattc catattggca ccaaaatacc
    23041 aggttccgct cacaccgcgc gcaattgcgc caacattaaa attcgacaat ggcgtacgcg
    23101 cacaggccgc cgccagcgga agtagtgcga atgccagcgc gtcttcgtcc agccccgttg
    23161 cgctcttcag cgatgagact tgctccccgg tcaacaaagc ggggaagtac ttgtctgcca
    23221 gaataggttc cagtgcagat tgcaagttat ccgcaagttg ggcaaaagcg gtttgaaaac
    23281 gtggatgcat ggcgttgcct cataatcatg taatgggata acagtttacg aacccgttat
    23341 ggctttatat gtgatctgaa tctcattaat tatgcaacgc gtgaaactta catcaaaaat
    23401 gcgcgacgca tcgcaaattt tagcccatta ctgccagaat aatcgggaaa aggaacggtg
    23461 cgatcagcga agtaattatc ccgcataaca ccagtgccag cgaactaaac gcaccttcct
    23521 gataatccag ctcggcgcag cgcgccgtac cgagggcgtg cgaggctgtc cccatcgcca
    23581 gaccgcgcgc agctttggta cgaatacgca tcgcattaag caatgtatgg ccaaacacag
    23641 cgccgaggat gccgacgaaa atgacgcaaa ctgcgctgat tgccggaata ccgccaatac
    23701 tgccgccaac cgccattgca ataggcgtgg tgactgattt cggcaggatt gacgcggcga
    23761 tttccggtga agcgcccatc aacaatgcca cagaagtccc cgtcaccatc gcaaccacgc
    23821 tgccgataaa acagatggtg atgatcgatt tccagcgcgc gcggatctgg tgtagctgct
    23881 catataaagg ataggccagc gcgacgaccg ccggttgcag cagatcgttt agcacttcgc
    23941 tacctttaaa gtagctgtcg taagagatgc cggtcagcat taaaaaagga atgatcacca
    24001 ccattgctac cagcaacggg ttaagcaagg gaaacttata ccgtgccgcc agtttgcggg
    24061 cggcaaaaaa gacaatcaac gtcagcggta atgaccacca gatattcgcc atcattcttc
    24121 tgatcctttc tgacctacga ctttacgttc accgtgcacc agttgcgaac tccagctcac
    24181 caccagaaaa accaccagcg tactgactgc acaggaaacc actaccgggc caaactgtgc
    24241 gcggagcaaa tcaaaatatt gcatgacgcc tacgccaatc ggcacaaaca atagcgccat
    24301 atagcgaatc agtacgtagc accccggatt gacccatttt gccggaagaa tttgcaaggc
    24361 cagcaggaca aacaggatca gcatcccgat gatgctgccc ggaatggtta ccggtagcag
    24421 tgaagcaatg aaaatgcctg catacaggca ggcataaatc aggacaaaag cacgtaaata
    24481 ttgccagata atgttcagtg tcttactcat aatcaacacc ccagatgaga tggattcatc
    24541 atacaattaa ttcgtaaaac gtgctaccga tcacattaag aaatcagcgc ttattttttc
    24601 gctcccgggc attaagcaat gagcagcgaa ctcaggcata catcaagcat agagagggat
    24661 tgcagtttgc agcaatccct ctttgcagac ctttaatccg ctgaccatag gcgctaacat
    24721 agcgcctcat ttttttgcgg gtgatgatat gcgtgtgtta ctggcaccga tggagggtgt
    24781 actcgactct ctggtgcgtg aattgctgac cgaagttaac gactacgatc tgtgcatcac
    24841 cgagtttgtc cgcgtggtgg atcaactgct gccaataaaa gtctttcatc gcatttgccc
    24901 tgagctacaa aatgccagcc ggacaccttc cggtacgctg gtgcgcgtgc agttgttagg
    24961 tcagttccca caatggctgg cagagaacgc cgcccgtgcg gtggagttag gttcctgggg
    25021 cgtggatctc aattgcggct gcccgtcgaa aacggttaac ggtagcggcg gtggggcgac
    25081 gttactcaaa gatcccgaac tcatctacca gggtgcaaaa gcgatgcgtg aagctgtacc
    25141 ggcgcatttg cccgtcagcg tgaaagtgcg tctgggatgg gacagcggtg agaagaaatt
    25201 tgaaatcgcc gatgcggttc aacaggctgg tgctacagag ctggtggtgc atgggcggac
    25261 gaaagagcag ggttaccgcg cggagcatat tgactggcag gcgattggcg agattcgcca
    25321 gcggctgaat attccggtga ttgccaacgg tgaaatctgg gactggcaga gtgcgcaaca
    25381 atgcatgacg atcagcggat gcgacgcagt gatgattggt cgcggggcgc tcaatattcc
    25441 caacctgagc cgggtggtaa aatataacga accgcgaatg ccgtggccgg aggtagttgc
    25501 tttgctgcaa aaatataccc gtctggaaaa gcagggcgat accgggttat atcacgttgc
    25561 gcggattaaa cagtggttga gttatttgcg taaagaatac gatgaagcaa cggaattatt
    25621 tcagcatgtt cgggtgttga ataattcccc tgatattgca agggctattc aggcaattga
    25681 tatcgagaaa ctctaaaaaa ccatagtgat gttttttgac aatgtgttag ctctttatca
    25741 ataggccgca cggttgtttg gtggcgtcaa aatcgccgag gcgttccccg aaggccgggg
    25801 cagcccacat ggatgtgggc tgagggcgcg ttttacaggg atgttacctc gcgcccgacc
    25861 cggtagccgt a
//
LOCUS       DACBVI010000058        25649 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.058, whole genome shotgun sequence.
ACCESSION   DACBVI010000058 DACBVI010000000
VERSION     DACBVI010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.058"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            228..335
                     /locus_tag="IU325_11555"
     CDS             228..335
                     /locus_tag="IU325_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HAP5809657.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIMNWLNKRK"
     gene            complement(422..2101)
                     /gene="bcsG"
                     /locus_tag="IU325_11560"
     CDS             complement(422..2101)
                     /gene="bcsG"
                     /locus_tag="IU325_11560"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HAP5809658.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2098..2289)
                     /gene="bcsF"
                     /locus_tag="IU325_11565"
     CDS             complement(2098..2289)
                     /gene="bcsF"
                     /locus_tag="IU325_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HAP5809659.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2286..3857)
                     /gene="bcsE"
                     /locus_tag="IU325_11570"
     CDS             complement(2286..3857)
                     /gene="bcsE"
                     /locus_tag="IU325_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HAP5809660.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEGITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQNSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            4129..4317
                     /locus_tag="IU325_11575"
     CDS             4129..4317
                     /locus_tag="IU325_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809661.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4329..5081
                     /gene="bcsQ"
                     /locus_tag="IU325_11580"
     CDS             4329..5081
                     /gene="bcsQ"
                     /locus_tag="IU325_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HAP5809662.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDDQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDSSQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSKS"
     gene            5078..7696
                     /gene="bcsA"
                     /locus_tag="IU325_11585"
     CDS             5078..7696
                     /gene="bcsA"
                     /locus_tag="IU325_11585"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HAP5809663.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWLILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNNRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            7707..10046
                     /gene="bcsB"
                     /locus_tag="IU325_11590"
     CDS             7707..10046
                     /gene="bcsB"
                     /locus_tag="IU325_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HAP5809664.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVVLLAWVLWRLLRIISRRRLNPDNE"
     gene            10053..11159
                     /gene="bcsZ"
                     /locus_tag="IU325_11595"
     CDS             10053..11159
                     /gene="bcsZ"
                     /locus_tag="IU325_11595"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HAP5809665.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWGRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            11141..14614
                     /gene="bcsC"
                     /locus_tag="IU325_11600"
     CDS             11141..14614
                     /gene="bcsC"
                     /locus_tag="IU325_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HAP5809666.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQWQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWSSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNQLIPLAKSQPPSMESAMVLRDGAKFEARAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKIINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            14696..16684
                     /gene="hmsP"
                     /locus_tag="IU325_11605"
     CDS             14696..16684
                     /gene="hmsP"
                     /locus_tag="IU325_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HAP5809667.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQQRLTEESDILNALENHQFAIWLQPQVEMNSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            16867..18153
                     /gene="dctA"
                     /locus_tag="IU325_11610"
     CDS             16867..18153
                     /gene="dctA"
                     /locus_tag="IU325_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctA"
                     /protein_id="HAP5809668.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            18373..19869
                     /locus_tag="IU325_11615"
     CDS             18373..19869
                     /locus_tag="IU325_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP5809669.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLEAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLSSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMTPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(19965..20894)
                     /locus_tag="IU325_11620"
     CDS             complement(19965..20894)
                     /locus_tag="IU325_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HAP5809670.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            21126..21893
                     /gene="pdeH"
                     /locus_tag="IU325_11625"
     CDS             21126..21893
                     /gene="pdeH"
                     /locus_tag="IU325_11625"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HAP5809671.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLSPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            21963..24023
                     /locus_tag="IU325_11630"
     CDS             21963..24023
                     /locus_tag="IU325_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAP5809672.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     EWNRPFAIRGDLGVVWERQKQETGWRSWVPWPHIHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDGNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(24205..25527)
                     /locus_tag="IU325_11635"
     CDS             complement(24205..25527)
                     /locus_tag="IU325_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5809673.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
BASE COUNT         6036 a         6559 c         7137 g         5917 t
ORIGIN      
        1 tgatggattt cgacgaacaa aaatggccgt agaatcagcg gcaagccgga aaggttccgg
       61 tgaggcgcaa tgttgcgggg gctttatccc tggtggcatt ggttgctgga gagagaaaac
      121 ccccgcacgt tgcaggtatg cacctgacaa caccacgggg gctaatcttg actctagacc
      181 actcaagaat agccgcgaaa cgttgtcatt acaacacagg cggctatatg acgctcgcag
      241 aactgggcat ggccttctgg catgatttag cggctccggt cattgctggc attcttgcca
      301 gtatgatcat gaactggctg aacaagcgga agtaacgtgt catgcgggcg tcaggctgcc
      361 gtaatggcaa tttgcgcccg gatcaggccg caggggtgaa actctgcggc ctttttcgtg
      421 cttactgcgg gtaaggcacc cagtcgccgc cgttcaggcg aacgtacggt ttatcctggt
      481 attgaataac tactgcattt gagttctctg agaccggtgc tgtttgtggc aacccactgg
      541 tgagtttttt ccagtcaaca ttgtcttcgg tgaaaatctt gccatcaaga acgcgaacca
      601 ccagatcgga gatagccagg aagctgctcg gttggtcgat gacaatcggt gccccctgat
      661 gtggtgcctt catgccgaag aatttcaccc caacggggac gtctgtgata gacgggctag
      721 ggatatcacg taggccagat acctgcattc tgtcgccctt cagcgcgccg ccgtgttccg
      781 gcaccacgac caccatcact ttacgacccg atttctccag ttcagtaaag aaggcgtcca
      841 gttcatcaaa gaatttctgc gcccgcgctt tgtaatccgc tgttttgctg acgcccggat
      901 aatggttgcc gtcatgcagt ggaagcgtgt tgtagaacgt ggcactacgg ctgtttttat
      961 ctttttcggt aacgtccagc cagcggttaa gcacggcggt atcgtcataa accggcgaac
     1021 catcaaagcc cagcaaaata accggcagat ttgtttgatc catcaattcg gtctgcatgc
     1081 cgccattttc gcgaacttct ttcaaaaaac cgccgaactg gccgttatgt cccatcatca
     1141 ggtgctgggt aaagcccagt ttcgacaggt tatcaaacag atagcagtcg ttatttgccg
     1201 gttgatacag attagtgtgc gaagtctgcc cgcagctggc gcgcagtaaa cggatcgccg
     1261 ccgggccact gtaggaggtg gcggagttaa agttcttgaa ctcaatatcg aaatgcgacc
     1321 acagcggatg cgacatcaac ccggcggctt caatatccga ccaggaaagc gaacagatat
     1381 taatcaccag tagttcaaat ggttgagcat cagcgggcag cgaagacggg aaggtcgatt
     1441 tacgtttcgc ctccgcgtta tagaaattat tcagccaggc gttaaggttc gccgttgttg
     1501 gcggtgcagt ttgtgcgggc atatcaccca ctaccggtgc gccacccgtc gccgcaacgg
     1561 ttgccgctgc gttgccaccc gtcgttgtta cagtggtcgt cggttgaccg gctggccaca
     1621 aggagaaact tggtcccgcc agggtaagta cgttcagcca tagcagaatg gcaaccacaa
     1681 aaacggtaat gcgaatccat tgtgacagga ataaccaggc cactaataaa acaaaaatgg
     1741 ccccaatcat ctgccagtta ataaagcgtg tgacaaggtc gattaaataa tcggtactga
     1801 accccgccac ctgcgaaccc tggctcatta tgctttccgg gccaggcaac caggtgtcat
     1861 gccagaacaa agcaaagccg atcggcaggg caatccagtg gcgtaagcga tgcaggctgt
     1921 agcgcggaat gggcatcagc agaaacgcgg caaacaccag attgaggagc ggatggaagt
     1981 taagatatcc cgcccacaac aggccgaact taaccagaaa atagaagttc cagccggaaa
     2041 ggccgcgcca gtattgccag agggaagaag gcatggcggt attttgcgta aattgagtca
     2101 ttttttggtt gccctggctt tttccgtgcg gcgtaacgtc ccggccggtt taacataacg
     2161 aggtttagca aagaacaaac gtaaggtgtc gcgaatgcgt cgcaaagagt gccgcgccag
     2221 atagcccagc gggaaaaata tcagtgcgca aacgacaata atttcaatga tatcgctgat
     2281 ggtcatcatg atgagcgctc cacagcatca tctaacagtc gcattggttc tggtattcgt
     2341 cgccagtggc gaccatcgtg ctcggcatta atgaccggtt tagaattttg cgttaaaggc
     2401 agcggcatgc cccattgttc tggggcaagc aggcgcatct gcaccagctc ggcactgatt
     2461 tgatcatctt caaaccagac catacggttt gagaaaatgt cgccagtcgg caatgggaaa
     2521 atatgattca acgcggtatc cagatcgtta atccgacaga atgagagaaa cagcaccagc
     2581 cgattaccgc caatagtcat gatatcgccg gtgcggttag ggcgacacag cgtcagggct
     2641 tgctcaacgc ggatacccgg taccggacgt agggcaacca gaacgccttt accgtgggca
     2701 ggtaatagag ggttattcat catgttgttg acggcattac agaacacatc ccacttctgg
     2761 aaaccacgca gtttgagcgg ctgggtcatt gacagcaagg tagtgatacc ttccggcaca
     2821 tagcgactaa acttctgccc ttgcacgctt tcgatcatcg tcagacagcg ggagagtggg
     2881 gcattccatg ggataaccat atttgcaccg caggccaata acaaacgttc atcggtggcg
     2941 cgcaggctag cggtattttc ccgtacgagg attttcatcg cactaccgcg ctggcgacgc
     3001 agagtatgaa tgctgcgggc cagtggctcg atttgcgcat tttgttgtaa agaaaagacc
     3061 accgtcgccg cctgagcggt acgggcttca ttgaacagga cttcgttatt gttgaacagt
     3121 tgccagtgtt ctgatagcgg cggcgcacct tccagtacag caacattact cagaatgcgt
     3181 ttttcgtcgc tgcgtggttg gatctccgcc tcttcgcttt gaactaatgt ccagatacca
     3241 ttttgttgct gaacgctaag ctgctgacgg gcgctaaccc ctttttcgtt gcaccagaag
     3301 gcaatatcca gcaaatgttg gtcgccctga aaacgcaaac tggcaagacc aaaaagtgaa
     3361 cggtattcct caagcaacaa tgaaaattgt ttatcgttat tatttccggg attaattacc
     3421 aaaagcgaac aatggtttaa cctgctccat ttattcattt tatccaacca tgagcgaagc
     3481 cgctcggcag gaatgttttg ccatgcgtta tttgcgcaaa caagaataaa aaggtaatta
     3541 tggggatcaa tagaacactg caaatcacgg gccaaatagt atagaccttt ttcatgattt
     3601 agcattgaaa ataattttat tttttccggt ccttgagaat catctaattg aaagattttc
     3661 gccggatcgc tgtccatgct aatgaccgcg acgtgtgcgg tttcagcctg ggatgcaatt
     3721 gtttgattcg ccagactgat agcatcttca tggcgatcga cgttaaacca ccagacgccg
     3781 cctgctggca tatgtcgcag ctcatcccat aatgatgaga taccgataga gaatacaggg
     3841 tccacaatgt ccctcatgtc atcattttgt ctgattatca gtttactagc gaaagctaag
     3901 aaataaacct accattgaaa ttaaaactta tcagatttag catgtgaatc aatttagcag
     3961 gtcacaagac gtgtgatttt agtcttttcg tttttaactt tttttgttgc gtaacggaaa
     4021 gtcaaaaagt gagcaaattc ccgtctcgcc gtaaacaata aaccggcgaa atgctcacgg
     4081 ttagaattgt ctcatgaacg gtacggttat ttcataggga tcaagcaaat gaataacaat
     4141 gaaccagata ctctgcctga tcccgcgata ggctatatct tccagaatga tattttggcg
     4201 ttaaagcagg cattttcact gcctgatatt gattatgccg atatttccca acgcgaacag
     4261 ttggccgcgg cattaaaacg ctggccgttg ctggcagagt ttgcgcaaca aaagtagggg
     4321 attggtgaat ggccgtactg ggattgcagg gggtgcgggg aggcgtgggg acaacaacca
     4381 tcaccgccgc attagcctgg tcattacaaa tgttgggaga aaatgtcctg gtggtcgatg
     4441 cctgcccgga caacttgttg cgcctgtcat ttaacgttga ttttacccac cgtcagggct
     4501 gggccagagc tatgctggat gaccaggact ggcgtgacgc cgggttgcgc tatacctcgc
     4561 agctcgattt gctgcctttt ggtcagttat ccattgaaga acaagaaaat ccacagcact
     4621 ggcaaacccg gctgagcgat atttgctccg gcttacagca actaaaagcc agcgggcgtt
     4681 accagtggat tttaatcgac ttaccgcgtg attcctcgca gataacccac cagctgctga
     4741 gtttgtgcga tcactcgctg gcaatcgtca atgtggatgc caactgccat atccggctgc
     4801 atcagcaggc gctgccggat ggcgcgcata ttttgattaa tgacttccgt atcggcagtc
     4861 aagttcagga cgatatttac cagctttggt tgcaaagcca gcgccgatta ctgccgatgc
     4921 tcattcatcg tgatgaagcg atggctgaat gcctggcggc taagcaacca gtcggtgaat
     4981 atcgcagtga tgcgctggcg gctgaagaga tactgacgct ggctaactgg tgcctgttga
     5041 actattccgg gctgaaaacg ccagtcggga gtaaatcatg agtatcctga cccggtggtt
     5101 gcttatcccg ccggtcaacg cgcggcttat cgggcgttat cgcgattatc gtcgtcacgg
     5161 cgcgtcggct ttcagcgcga cgctcggctg tttttggttg atcctggcct ggatttttat
     5221 tccgctggag cacccgcgct ggcagcgtat tcgcgcagaa cataaaaacc tgtatccgca
     5281 tatcaacgcc tcgcgtccgc gtccgctgga cccggtccgt tatctcattc aaacatgctg
     5341 gttactgatc ggtgcatcgc gcaaagaaac gccgaaaccg cgcaggcggg cattttcagg
     5401 tctgcagaat aatcgtggac gttaccatca atggatgaac gagctgcctg agcgcgttag
     5461 ccataaaaca cagcatcttg atgagaaaaa agagctcggc catttgagtg ccggggcgcg
     5521 gcggttgatc ctcggtatca tcgtcacctt ctcgctgatt ctggcgttaa tctgcgttac
     5581 tcagccgttt aacccgctgg cgcagtttat cttcctgatg ctgctgtggg gggtagcgct
     5641 gatcgtacgg cggatgccgg ggcgcttctc ggcgctaatg ttgattgtgc tgtcgctgac
     5701 cgtttcttgc cgttatatct ggtggcgcta cacctctacg ctgaactggg acgatccggt
     5761 cagcctggtg tgcgggctta ttctgctgtt cgccgaaacg tacgcgtgga ttgtgctggt
     5821 gctcggctac ttccaggtag tatggccgct gaatcgtcag ccggtgccat tgccgaaaga
     5881 tatgtcgttg tggccgtcgg tggatatctt tgtcccgact tacaacgaag atctcaacgt
     5941 ggtgaaaaat accatttacg cctcgctggg tatcgactgg ccgaaagaca agctgaacat
     6001 ctggatcctt gatgacggcg gcagggaaga gtttcgccag tttgcgcaaa acgtgggggt
     6061 gaaatatatc gcccgtacca ctcatgaaca tgcgaaagcg ggcaacatca acaatgcgct
     6121 gaaatatgcc aaaggcgagt tcgtgtcgat tttcgactgc gaccacgtac caacgcgatc
     6181 gttcctgcaa atgaccatgg gctggttcct gaaagaaaaa caactggcga tgatgcagac
     6241 gccgcaccac ttcttctcac cggacccgtt tgaacgcaac ctggggcgtt tccgtaaaac
     6301 acctaacgaa ggcacgctgt tctatggtct ggtgcaggat ggcaacgata tgtgggacgc
     6361 cactttcttc tgcggttcct gtgcggtgat tcgccgtaag ccgctggatg aaattggcgg
     6421 cattgctgtc gaaactgtga ctgaagatgc gcatacctct ttgcgcctgc accgtcgtgg
     6481 ctacacctct gcatatatgc gtattccgca ggcggcgggg ctggcgaccg aaagtctgtc
     6541 ggcgcatatc ggtcagcgta ttcgctgggc gcgcgggatg gtgcaaatct ttcgtctcga
     6601 taacccgctc accggtaaag ggctgaagtt tgctcagcgg ctatgttacg tcaacgccat
     6661 gttccacttc ttgtcgggca ttccacgact gatcttcctg actgcgccgc tggcgttcct
     6721 gctgctgcat gcctacatca tctatgcgcc agcgttgatg attgccctgt tcgtgctgcc
     6781 gcatatgatc catgccagcc tgaccaactc caagatccag ggcaaatatc gccactcttt
     6841 ctggagtgaa atctacgaaa cggtgctggc gtggtatatc gcaccaccga cgctggtggc
     6901 gctgattaac ccgcacaaag gcaaatttaa cgtcaccgcc aaaggtggac tggtggaaga
     6961 agagtacgtc gactgggtga tctcgcggcc ctacatcttc cttgttctgc tcaacctggt
     7021 gggcgttgcg gtaggcatct ggcgctactt ctatggcccg ccaaccgaga tgctcaccgt
     7081 ggtcgtcagt atggtgtggg tattctacaa cctgattgtt cttggcggcg cagttgcggt
     7141 atcggtagaa agcaaacagg tacgccgatc gcaccgcgtg gagatgacga tgcccgcggc
     7201 aattgcccgc gaagatggtc acctcttctc gtgtaccgtt caggatttct ccgacggtgg
     7261 tttggggatc aagatcaacg gtcaggcgca gattctggaa gggcagaaag tgaatctgtt
     7321 gcttaaacgc ggtcagcagg aatacgtctt cccgacccag gtggcgcgcg tgatgggtaa
     7381 tgaagttggg ttgaaattaa tgccgctcac cacccagcaa catatcgatt ttgtgcagtg
     7441 tacgtttgcc cgtgcggata catgggcgct ctggcaggac agctacccgg aagataagcc
     7501 gctggaaagt ctgctggata ttctgaagct cggcttccgt ggctaccgcc atctggcgga
     7561 gtttgcgcct tcttcggtga agggcatatt ccgtgtgctg acttctctgg tttcctgggt
     7621 tgtatcgttt attccgcgcc gcccggagcg gagcgaaacg gcacaaccat cggatcaggc
     7681 tttggctcaa caatgatgat aacgcgatga aaagaaaact attctggatt tgtgcagtgg
     7741 ctatggggat gagtgcgttc ccctctttca tgacgcaggc gacgccagca acgcaaccac
     7801 tgatcaatgc tgagccagct gtagccgccc agacggaaca aaatccgcag gtggggcaag
     7861 tgatgccagg cgtgcagggc gctgatgccc ccatcgtggc gcagaacggt ccttcgcgtg
     7921 atgtgaagct gacctttgcg caaattgcgc ccccgccggg cagcatggtg ctacgtggca
     7981 ttaacccgaa cggcagcatt gagtttggta tgcgcagcga tgaagtggtg acgaaggcga
     8041 tgctcaacct cgaatacacg ccatcgccat cgttactgcc tgtccagtcg cagttaaagg
     8101 tttatctcaa tgatgaactg atgggcgtgc tgccagtgac caaagaacag ttgggtaaaa
     8161 aaacgctggc acaaatgccc attaacccac tgtttattac cgacttcaac cgtgtgcggc
     8221 tggagtttgt cggtcattat caggacgtgt gcgaaaaccc ggccagcacc acgctttggc
     8281 tggatgttgg gcggagcagt ggactggatt tgacctatca gaccctgaat gtgaagaatg
     8341 acctgtcaca cttcccggta ccattctttg acccgcgtga taaccgtact aacaccttgc
     8401 cgatggtctt tgcgggtgcg ccggatgttg ggctgcaaca agcatctgcc attgtcgcct
     8461 cgtggtttgg ttcgcgttct ggctggcgtg ggcagaactt cccggtgctc tataaccaat
     8521 tgccggatcg caatgccatc gtttttgcca ccaatgacaa acgcccggac ttcctgcgcg
     8581 atcatccggc ggtaaaagcc ccggtgattg agatgatcaa ccatccgcag aatccttatg
     8641 tcaaactgct ggtggtgttt ggtcgtgacg acaaagacct gttgcaggca gcgaaaggta
     8701 tcgctcaggg taacattctg ttccgtggtg aaagcgtggt agtgaatgaa gtgaaaccgc
     8761 tgctaccgcg taagccgtac gatgcgccga actgggtgcg taccgatcgt ccggttacct
     8821 ttggtgaact gaaaacctat gaagaacagt tacaatccag cggtcttgag ccagcagcga
     8881 ttaacgtttc gctaaacctg ccgccggatc tctacctgat gcgcagtacc ggcattgata
     8941 tggatattaa ttatcgctac accatgccgc cggtgaaaga cagttcgcgg atggatatca
     9001 gcctgaataa ccagttcctg caatccttca acctgagcag caaacaggag gcgaatcgcc
     9061 tgctgctgcg gattccggta ttacaaggtt tgctggatgg caaaacggat gtctctattc
     9121 cggcgctgaa actgggcgcg accaaccagc tgcgctttga ctttgagtat atgaacccga
     9181 tgccgggcgg ttcggtggat aactgcatta ccttccagcc ggtgcagaat catgtggtga
     9241 ttggtgatga ttccaccatc gacttctcga agtattacca cttcatcccg atgccggatc
     9301 tacgcgcctt tgctaacgcg ggcttcccgt tcagccggat ggcggatctg tcacaaacca
     9361 tcaccgtgat gccgaaaacg cctaacgaag cacagatgga aaccttgctg aatactgttg
     9421 gttttatcgg cgcacagacg ggcttcccgg cgattaatct gacggtgacc gatgatggca
     9481 gcaccattca gggcaaagat gccgacatca tgatcatcgg tggtatcccg gacaaattaa
     9541 aagacgacaa gcagatcgac ctgctggtgc aggcgaccga aagctgggtg aaaacgccga
     9601 tgcgccagac cccgttcccc ggcattgtgc cggacgagag cgatcgcgcg gcagaaaccc
     9661 agtcaacgct gacctcttcc ggtgcgatgg cggcggtgat tggcttccag tcgccgtata
     9721 acgaccagcg tagcgtgatt gcgctgctgg cagatagccc acgcggttat gaaatgctta
     9781 acgatgcggt gaacgatagc ggcaaacgcg ccaccatgtt cggttcggtc gcggtgatcc
     9841 gcgagtccgg tatcaatagc ctgcgtgttg gcgacgtcta ttacgttggc catctgccgt
     9901 ggttcgaacg cttatggtat gccctggcaa accatccgat tctgctggcg gtgctggcgg
     9961 caatcagcgt cgtgctgctg gcctgggtac tgtggcgttt gctgcgtatt atcagccgtc
    10021 gtcgtcttaa cccggataac gagtaattga agatgaatgt gttgcgtagt ggactcgtga
    10081 cgatgctgct gctggctgcc tttagtgttc aggcagcctg tacctggcct gcctgggaac
    10141 agtttaaaaa ggattacatc agtcaggaag ggcgcgtcat cgaccccagc gacgcgcgca
    10201 aaatcaccac ttccgaaggg caaagttacg gcatgttctt tgccctggcg gctaacgacc
    10261 gtgcagcttt cgataatatt ctcgactgga cgcagaacaa tctcgctcag ggttctttaa
    10321 aagaacgttt gcccgcatgg ctgtggggca agaaagagaa cagtaagtgg gaagtgctgg
    10381 acagcaattc ggcctccgat ggtgatgtct ggatggcctg gtcgttgctg gaggcggggc
    10441 gtttgtggaa agagcagcgt tataccgaca tcggcagcgc gttgttaaaa cgtattgcgc
    10501 gggaggaagt ggtgacggtg cctgggctgg gctccatgtt gttgccgggc aaagtgggat
    10561 ttgcagagga taacagctgg cgttttaacc ccagctacct gccgccgacg ctggcgcagt
    10621 atttcacccg ctttggcgcg ccgtggacta cgctgcgcga aaccaatcaa cgtttattgc
    10681 tggaaaccgc cccgaaaggc ttttcgccag actgggggcg ctatgagaaa gacaaaggct
    10741 ggcagctaaa agccgaaaaa acattgatca gcagctacga cgcgatccgc gtttacatgt
    10801 gggtaggcat gatgcctgac agcgatccgc aaaaagcgcg gatgctcaac cggtttaaac
    10861 cgatggcgac attcaccgag aaaaacggct atccgccaga aaaagtggat gtggctacgg
    10921 ggaaagcgca gggtaaagga ccggtcggtt tttctgccgc catgctgccc tttttacaaa
    10981 accgcgatgc gcaggccgtt cagcgccagc gcgtggccga taactttccc ggcagcgatg
    11041 cctattacaa ctatgtgctg accctgtttg gacaaggctg ggatcaacac cgtttccgct
    11101 tctcgacaaa aggtgagtta ttacctgact ggggccagga atgcgcaaat tcacactaaa
    11161 catattcacg ctttccctcg gtctggccgt catgccgatg gtcgaggcag caccaaccgc
    11221 tcagcaacag ttgctggagc aagttcggtt aggcgaagcg acccatcgtg aagacctggt
    11281 gcaacagtcg ttgtatcggc tggaacttat tgatccgaat aacccggacg tcgttgccgc
    11341 ccgtttccgt tctctgttac gtcagggcga tattgatggc gcgcaaaaac agctcgatcg
    11401 gctgtcgcag ttagcgccga gttcaaatgc gtataaatcg tcgcggacca caatgttgct
    11461 ttccacgccg gatggtcgtc aggcactgca acaggcaaga ttgcaggcga cgactggtca
    11521 tgcagaagaa gctgtggcga gttacaacaa actgttcaac ggtgcgccgc cggaaggtga
    11581 cattgccgtc gagtactgga gtacggtggc gaaaattccg gctcgccgtg gcgaagcgat
    11641 taatcagcta aaacgcatta atgcggatac gccgggcaat acgggcctgc aaaacaatct
    11701 ggcgctattg ctgtttagta gcgatcgccg tgacgaaggt tttgccgtcc tggaacagat
    11761 ggcgaaatca aacgccgggc gcgaaggggc ctctaaaatc tggtacgggc agattaaaga
    11821 catgcccgtc agcgatgcca gtgtgtcggc gctgaaaaaa tatctctcga tctttagtga
    11881 tggcgatagc gtagctgcgg cgcaatcgca actggcagaa cagcaaaaac agctggccga
    11941 ccctgctttc cgcgctcgtg cgcaaggttt agcggcggtg gactctggta tggcgggtaa
    12001 agccattccc gaactacaac aggcagtgcg ggcgaacccg aaagacagtg aggctctggg
    12061 ggcgctgggc caggcgtatt ctcagaaagg cgatcgcgcc aatgcggtgg cgaatctgga
    12121 aaaagccctc gccctggatc cgcacagcag caacaacgac aaatggaaca gtctcctgaa
    12181 agtaaaccgc tactggctgg cgatccagca gggcgatgct gcgctgaaag ccaataatcc
    12241 tgaccgggca gaacgcctgt tccagcaggc gcgtaatgtc gataacaccg acagctatgc
    12301 agtgctgggg ctgggcgatg tggcgatggc gcgaaaagat tatcccgccg ccgaacgtta
    12361 ttatcagcag accttgcgta tggacagcgg caacactaac gccgtgcgcg ggctggcgaa
    12421 tatataccgc cagcaatcgc cagaaaaagc tgaagcgttt atcgcctcgc tctctgccag
    12481 tcagcggcga agcattgatg atatcgaacg cagcctgcaa aacgaccgtc tggcacagca
    12541 ggcagaggca ctggaaaacc agggcaaatg ggcgcaggcg gcagcacttc agtggcaacg
    12601 actggcgctg gatcccggca gcgtatggat tacttaccga ctttcgcagg atctctggca
    12661 ggccggacaa cgcagccagg ctgatacatt aatgcgcaat ctggcacagc agaagccaaa
    12721 cgacccggag caggtttacg cttacgggct gtatctctct ggtcatgacc aggacagagc
    12781 ggcgctggcg catatcaaca gcctgccgcg cgcgcagtgg agcagcaata ttcaggagct
    12841 ggttaatcga ctgcaaagcg atcaggtact ggaaaccgct aatcgcctgc gagaaagcgg
    12901 caaagaagct gaagcggaag cgatgctgcg ccagcaacca ccttccacgc gcatagacct
    12961 cacgctggct gactgggcgc aacaacgacg tgattacacc gccgctcgcg ctgcatatca
    13021 gaatgtcctg acgcgggagc caactaacgc cgatgccatt cttggtctga cggaagtgga
    13081 tattgctgcc ggtgacaaag cggcggcacg tagccagctg gcgaaactgc ccgctaccga
    13141 taacgcctcg ctgaacacac agcggcgcgt ggcgctggca caggcgcagc ttggcgatac
    13201 cgcagcggcg cagcagacgt ttaatcagtt gatcccgctg gcaaaatctc agccgccgtc
    13261 gatggaaagt gcgatggtgc tgcgcgacgg tgcgaagttt gaagcgcggg cgggcgatcc
    13321 aaagcaggcg ctggaaacct acaaagacgc tatggtcgca tccggtgtga ccacgacgcg
    13381 tccgcaggat aacgacacct ttacccggct gacccgtaac gacgagaaag atgactggct
    13441 gaaacgcggc gtgcgcagcg atgcagcgga cctctatcgc cagcaggatc ttaacgtcac
    13501 ccttgagcac gattattggg gatcgagcgg caccggtggt tactccgatc tgaaagcgca
    13561 cactaccatg ttgcaggtgg atgcgccgta ttctgacggg cggatgttct ttcgcagtga
    13621 tttcgtcaat atgaacgtcg gcagtttctc cactaatgcc gatggcaaat gggatgacaa
    13681 ctggggcacc tgtacattac aagactgtag cggcaaccgc agccagtcgg attccggtgc
    13741 cagcgtggcg gtcggctggc gaaatgacgt ctggagctgg gatatcggca ccacgccgat
    13801 gggcttcaat gtggtggatg tggtcggcgg catcagttac agcgatgata tcgggccgct
    13861 gggttacacc gttaacgccc atcgtcggcc catctccagt tctttgctgg cctttggcgg
    13921 gcaaaaagac tccccgagca ataccgggaa aaaatggggc ggcgtgcgtg ccgacggcgt
    13981 ggggctaagc ctgagctacg ataaaggtga agcaaacggc gtctgggcat cgcttagcgg
    14041 tgaccagtta accggtaaaa atgtcgaaga taactggcgc gtgcgctgga tgacgggcta
    14101 ttactataag atcatcaacc agaataatcg ccgtgtgacc attggtctta acaatatgat
    14161 ctggcattac gacaaagacc tgagtggcta ctcactcggt cagggcggtt actacagccc
    14221 gcaggaatac ctgtcgtttg ccataccggt gatgtggcgg gagcgcacgg aaaactggtc
    14281 gtgggagctg ggggcgtctg gctcgtggtc gcattcacgc accaaaacca tgccgcgtta
    14341 tccgctgatg aatctgatcc cgaccgactg gcaggaagaa gctgcgcggc aatcgaacga
    14401 tggcggcagc agtcagggct ttggctacac ggcgcgggca ttacttgaac gacgtgttac
    14461 ttccaactgg tttgtcggca cggcaattga tatccagcag gcgaaagatt acgcacccag
    14521 ccatttcctg ctctacgtac gttattccgc cgccggatgg cagggtgaca tggatttacc
    14581 gccgcagccg ctgatacctt acgccgactg gtaagttttc agatagcgcc tctcttaatg
    14641 ccgctgcgat cgggtatact cgggcggcaa tctgggattt ccggggggag acaatttgcg
    14701 cgtaagtcgc tcgttaacaa tcaagcagat ggcaatggtg gcagccgttg tcctggtgtt
    14761 cgtttttatt ttttgcaccg ttttgctgtt ccatctggtc cagcagaatc gctataacac
    14821 ggctacgcaa ctggaaagca ttgctcgctc tgtccgcgaa cccttatctt cagctatttt
    14881 gaaaggcgat attcccgaag cggaagctat tcttgccagc attaaaccgg caggcgtggt
    14941 cagccgtgcc gatgtggtgc tgcccaacca gttccaggca ctgcgtaaaa gttttattcc
    15001 agagcgtccg gtgccggtaa tggttactcg cctgtttgag ctaccggtgc aaatctcgct
    15061 gggcgtttac tcgctggaac gtccggcaaa cccgcagcca attgcctatc tggtgctaca
    15121 ggcggattcc ttccgcatgt ataagttcgt gatgagcacc ctctcaacgt tagtgaccat
    15181 ttacttactt ttgtcgctta tcctgaccgt cgccatcagc tggtgtatta atcgactgat
    15241 tttacatcct ttacgcaata ttgctcgcga acttaacgcc atcccagccc aggagcttgt
    15301 tggtcaccaa ctggcattac cgcgtctgca tcaggatgat gaaatcggta tgttggtgcg
    15361 cagttacaac ctcaaccagc aattgctgca gcgccattat gaagaacaga acgaaaacgc
    15421 gatgcgcttc ccggtgtcgg atttgccgaa caaagccttg ctgatggaga tgctggagca
    15481 ggttgtcgcg cgtaaacaaa ccaccgcgct gatgatcatc acctgtgaaa ccctgcgtga
    15541 tactgcgggc gtgctgaaag aggcgcaacg agaaattctg ctgctgacgc tggtggaaaa
    15601 actcaaatcg gtactgtcgc cacgtatgat cctcgcgcag attagtggtt atgactttgc
    15661 tgtcattgcc aatggtgtac aggaaccgtg gcacgcaatc actttaggtc agcaagtgct
    15721 cactatcatg agcgagcgcc tgccgattga acgtattcaa ctccgtccgc actgtagcat
    15781 tggcgtggcg atgttctacg gcgatctcac cgccgaacag ctttacagtc gcgctatttc
    15841 tgcggcattt accgctcgcc ataaaggcaa gaatcagatt cagttctttg atccgcagca
    15901 gatggaagcc gcccagcagc ggttgacgga agagagcgat atccttaatg cgctggaaaa
    15961 tcatcagttt gcgatttggt tacagccaca ggtcgagatg aacagcggta aactggtcag
    16021 tgcggaagtg ttactgcgta tccagcaacc ggatggcagt tgggatctgc cggatggctt
    16081 aatcgatcgc attgagtgct gtgggctgat ggttaccgtc ggtcactggg tgctggaaga
    16141 gtcctgtcga ttgcttgcag cctggcaaga gcgcggcatt atgctgccct tgtcggtaaa
    16201 cctctctgcg ctgcaactga tgcacccgaa tatggtggcg gatatgctgg aactgttaac
    16261 ccgctatcgc attcagccgg gaacactgat tctggaagtg acagaaagcc gacgtattga
    16321 cgaccctcat gctgcggtgg caatcctccg tccgctgcgc aatgccggag ttcgggtggc
    16381 gctggatgat ttcggcatgg gctacgcagg gctgcgtcag ctgcagcata tgaaatcgtt
    16441 gccaatcgac gtgttaaaaa tcgacaaaat gtttgttgaa ggcttgccgg aagatagcag
    16501 catgattgct gcaattatca tgttggcgca aagcctgaac ttacaaatga ttgccgaagg
    16561 cgtggagact gaagcacaac gcgactggct ggcaaaagcg ggcgttggta ttgcccaggg
    16621 cttccttttt gctcgcccac tccctattga aatcttcgaa gagagttacc tggaagaaaa
    16681 gtagctaccc caaactgatt acaaaacttt aaaaagtgct ggtttgtgcg agccagctca
    16741 aactttttaa cctttttgtt tcaattatga tccaggtaca tttctgtgat gttgtctggg
    16801 tgttatttta aggccgcagg taccccataa ccttacaaga cctgtggttt tactaaagga
    16861 caccctatga aaacctctct gtttaaaagc ctttactttc aggtcctgac agcgatagcc
    16921 attggtattc tccttggcca tttctaccct gaaataggcg agcaaatgaa accgcttggc
    16981 gacgggttcg ttaagctcat taagatgatc atcgctcctg tcatcttttg taccgtcgta
    17041 acgggcattg cgggcatgga aagcatgaag gcggtcggtc gtaccggcgc agtcgcactg
    17101 ctttactttg aaattgtcag taccatcgcg ctgattattg gtcttatcat cgttaacgtc
    17161 gtgcagcctg gtgccggaat gaacgtcgat ccggcaacgc ttgatgcgaa agcggtagcg
    17221 gtttacgccg atcaggcgaa agaccagggc attgtcgcct tcattatgga tgtcatcccg
    17281 gcgagcgtca ttggcgcatt tgccagcggt aacattctgc aggtgctgct gtttgccgta
    17341 ctgtttggtt ttgcgctcca ccgtctgggc agcaaaggcc aactgatttt taacgtgatc
    17401 gaaagtttct cgcaggtcat cttcggcatc atcaatatga tcatgcgtct ggcacctatt
    17461 ggtgcgttcg gggcaatggc gtttaccatc ggtaaatacg gcgtcggcac actggtgcaa
    17521 ctggggcagc tgattatctg cttctacatc acctgtatcc tgtttgtggt gctggtactg
    17581 ggttcaatcg ctaaagcgac gggtttcagt atcttcaaat ttatccgcta catccgtgaa
    17641 gaactgctga ttgtactggg gacttcatct tccgagtcgg cgctgccacg tatgctcgac
    17701 aagatggaga aactcggctg ccgcaaatcg gtggtggggc tggtcatccc gacaggctac
    17761 tcgtttaacc ttgatggcac atcgatatac ctgacaatgg cggcggtgtt tatcgcccag
    17821 gccactaaca gtcagatgga tatcgtccac caaatcacgc tgttaatcgt gttgctgctt
    17881 tcttctaaag gggcggcagg ggtaacgggt agtggtttta tcgtgctggc ggcaacgctc
    17941 tctgcggtgg gccatttgcc ggtagcgggt ctggcgctga tcctcggtat tgaccgcttt
    18001 atgtcagaag ctcgtgcgct gactaacctg gtcggtaacg gcgtagcgac cattgtcgtt
    18061 gctaagtggg tgaaagaact ggaccacaaa aaactggacg atgtgctgaa taatcgtgcg
    18121 ccggatggca aaacgcacga attatcctct taatctcacc acttatgccc gcactccctt
    18181 ccccgagtgc gggtgtttgc gcataattga cccctgtgcc tgaacttacc acctgggtga
    18241 gttcaggttt atttcacttc ttccgtgaca ttttcatgtt cttgcggtct aacacgaagt
    18301 gtttttaacg ttatttagac tgaccgacaa cgggcagttt tttattacca ggatagttga
    18361 tcaggggttc acatgcaggg cacaaaaatt cgacttttag cgggcggttt gctgatgatg
    18421 gccactgctg gctatgtgca ggcagatgcg ctccagcctg atccagcatg gcaacagggg
    18481 acgctttcca acggtttaca gtggcaagtg ctgaccacgc cccagcgtcc cagcgatcgt
    18541 gttgaaattc gcctgctggt taataccggt tcgctcgccg aaagtacaca acagagcggt
    18601 tacagccacg ccatccctcg tattgcgtta acgcaaagcg gtggccttga agcagcacag
    18661 gcgcgttcat tgtggcagca ggggatcgac cctaaacgcc cgatgccgcc ggtaattgtc
    18721 tcttatgaca ccacgctgtt taatctgagt ttgcccaata accgtaacga cttgctgaaa
    18781 gaagcgctct cttatctggc aaatgccact ggcaaactga ctatcacgcc agaaaccatc
    18841 aaccacgcgc tgcaaagtca ggacatggtg gcaacctggc ctgccgatac taaagagggc
    18901 tggtggcgct atcgtctgaa aggatcaacc ttgttaggtc acgatcctgc cgatccgctg
    18961 aaacaacccg ttgaagcgga aaagattaaa gatttctatc agaaatggta taccccggat
    19021 gcaatgacgc tgctggtggt gggaaacgtg gatgcgcgct cggtcgtcga ccaaatcaat
    19081 aaaacgtttg gcgaactgaa aggcaaacgt gaaacaccgg ctccggtgcc gacgctttct
    19141 ccgctgcgtg cggaagcggt gagtattatg actgacgcgg tgcgtcagga ccggttatca
    19201 atcatgtggg atacgccgtg gcagccgatt cgtgaatcag ccgcactgct gcgctactgg
    19261 cgtgcggacc tggcccgtga ggcgctgttc tggcatgttc agcaagcgtt aagcgccagt
    19321 aacagcaaag atatcggtct tggatttgac tgtcgtgtgc tgtatctgcg tgcgcagtgt
    19381 gccattaaca tcgaatcacc aaacgacaag ctgagcagca accttaatct ggtggcgcgt
    19441 gaactggcga aggttcgcga taaaggtctg ccggaagaag agttcaatgc gttagtggcg
    19501 caaaagaaac tggagctgca gaaactgttt gccgcctatg cgcgagctga taccgatatt
    19561 ctgatggggc agcggatgcg ttcgttgcaa aatcaggttg tcgatatcgc gccggagcaa
    19621 tatcagaaac tgcgccagga cttccttaac agcctgacgg tggagatgtt aaatcaggat
    19681 ctgcgtcagc agttgtcgaa cgatatggcg ttaatactgc tgcagccgaa aggcgagccg
    19741 gaatttaaca tgaaagcgtt gcaggcggcc tgggatcaaa tcatgacccc atctactgcc
    19801 gctgcgacca cctctgtcgc cacggatgac gttcatcctg aagtgacgga tattccaccc
    19861 gcacagtaat gcctgatggt gatgagttag cacaaccatc aggtatttat ggatgagctg
    19921 gatagagggg taacacttta tccagccttt tgcatatgct gcttttacgc cggcatcgcc
    19981 tcacgcggga taatcgcgcc gcgatactga ataacggtac ttgcggtcag gtgcccgcgt
    20041 ttcgccgcgt tttccgcact gccgcccgtc aaacgtaccg ccagataacc ggcactgaaa
    20101 gagtcgccag ctgcggtggt atcgatcact ttttctttcg gcagtttcac cgccggaaca
    20161 tccactaacc cttcgccagc aatggacacc aggcaagaat ccgccccgcg tttcaccacc
    20221 acttctttca cgcccgcgtt atgggtgcgc gcaatgacgt cttccaccgg ctgttgaccc
    20281 cacagcgcgt cttcgtcgtc cagcgtcagg aaggcgatat ccgtgcattc cagcatttgc
    20341 tggtacacct gctgtgtctc ttctttgctg gcccacaggc gcggacgata gttattgtcg
    20401 aaaatcactt ttccgccgtt ggcgcggcat tcgcgcaaca aagaaagcag tttctcgcgg
    20461 ctggtcgggc ttaagatcgc caggctaatc ccgctcaggt agagataatc gaaattcgcc
    20521 agctcttcgc aaatcgccgc agattgctcg ctctccagcc agaatttggc ggcggcttcg
    20581 ttacgccagt agtagaacgt acgttcgccg gtgctgtcgg tttcaatgta gtaaaggccc
    20641 ggcagacggt tttccatccg ttgggtcagg gaagtatcaa cgttctcgcc gtgccaggcg
    20701 tccagcatct gctggctaaa actgtccgtt cccagcgccg ttacgtaatg aacggttaat
    20761 gccgcaggat cgacctgacg ggcgatatag acggaagtgt tcagggtatc gccgccgaaa
    20821 ccgcgcttaa cgtccgcgcc tttctcggaa agctcaatca tgcattcgcc aatcacggca
    20881 atctttttgg acatagtcgt gaacctgatc tgtaaaaaat taacgttagt ttgcgctgtg
    20941 gtggtttgct ggtcaaccat attaaaacag tgttccatta tttttttgag ccagcacgta
    21001 ttcctgaaag attccgttgt ggagtgggga aatttactcc ggtcaggcgg taaagttctg
    21061 cccttgcgcg ccgataatct ttgtcgagtc cgggcagcat cacttttaaa cacaggacat
    21121 ctttgatgat aaggcaggtt atccagcgaa taagcaaccc tgaagcaagc atcgagagct
    21181 tgcaggaacg gcgtttttgg ttgcagtgtg agcgtgctta cacctggcag ccgatctatc
    21241 aaacatgcgg gcggttaatg gccgtggagc tattaacggt ggtcacgcat cccttgaacc
    21301 cttcgcaacg cctgtcgccg gatcgctatt ttactgaaat caccgtcagc catcggatgg
    21361 aggttgtgaa agagcagatt gatttgctgg cgcaaaaagc cgacttcttt atagagcacg
    21421 gcctgctggc atcggtcaat attgatggac ctacgctcat cgccctgcgt cagcaaccaa
    21481 aaatcctgcg ccagattgag cgtcttccct ggctgcgttt cgaactggtg gagcatatcc
    21541 gtctgccgaa agattcaacc tttgcctcga tgtgtgaatt tggcccgctg tggctggatg
    21601 attttggcac cgggatggca aatttctctg cactaagtga agtgcgttat gactacatca
    21661 aaatcgcgcg agaactgttt gtgatgctgc gtcagtcgcc ggaaggccgc acactctttt
    21721 ctcagctttt acatctaatg aatcgctatt gtcgcggggt gatcgtcgag ggcgtagaaa
    21781 cgccggaaga gtggcgcgat gtacagaact cgcccgcatt cgccgcacaa ggctggtttc
    21841 tttcacgccc ggcaccgata gaaacgctga atacggcggt tctggcgcta taagctgcct
    21901 cattttccga ctggctggac tatctttagg actggcacag gaagaatatg aggaagcgaa
    21961 cgatgagcaa ggcaggcaaa ataaccgctg cgatttcagg ggctttcttg ttgttgattg
    22021 tcgtggcgat cattttgatt gcaacttttg actggaatcg actcaaaccg accatcaacc
    22081 agaaagtctc tgcggagtgg aatcgtccgt tcgctatccg tggcgatctg ggcgtggtgt
    22141 gggagcgaca aaaacaagaa actggctggc gcagctgggt gccgtggccc catatacacg
    22201 cggaagacat cattctcggc aacccaccag atattcccga agtcacaatg gtgcatttgc
    22261 cacgcgtaga ggcaacgctg gccccgctgg cgctgctgac caaaacggtc tggctaccgt
    22321 ggatcaaact ggaaaagccc gacgcacgcc tgattcgcct ctctgaaaag aacaataact
    22381 ggacgtttaa tcttgccaac gatggcaaca aagacgcgaa tgcaaagccg tcggcatggt
    22441 cgtttcggct ggataatatt cttttcgatc aagggcggat cgccattgat gacaaagtaa
    22501 gcaaagcgga tctggaaatt tttgtcgatc cgttaggcaa gccgctgccg ttcagcgaag
    22561 ttactggatc gaaaggtaaa gcggataaag aaaaggtggg tgattacgtt tttggcctga
    22621 aggcgcaggg acgttataac ggtgaaccgc tcactggtac gggaaaaata ggcggtatgc
    22681 tggcgctgcg tggcgaaggt acaccatttc cggtacaggc tgattttcgc tctggtaaca
    22741 cccgtgttgc ttttgatggc gttgtgaatg acccaatgaa gatgggtggt gtcgatttac
    22801 ggcttaaatt ttctggcgat tcgctgggtg atctctatga actgacgggc gttctgctgc
    22861 ccgatacccc gccgtttgaa acggatggtc ggctggtagc gaaaatcgac actgaaaaat
    22921 cgtcggtctt tgattatcgc ggttttaatg ggcgcattgg cgatagcgat atccacggtt
    22981 ctctggtcta caccaccggc aagccacgac caaaactgga aggtgatgtc gagtcgcggc
    23041 aattgcggct ggcggacttg ggaccgttga ttggcgttga ttccgggaaa ggagctgaaa
    23101 agtcgaaacg gtctgaacag aagaagggtg aaaaaagcgt tcagcctgcg ggcaaagtgc
    23161 tgccttatga ccgcttcgaa accgataaat gggacgtcat ggatgccgat gttcgcttca
    23221 aagggcggcg cattgagcat ggcagtagcc tgccgattag cgatctttct actcatatca
    23281 tcctcaaaaa tgctgacctg cgcctgcaac cgctgaaatt tggcatggcg ggcggcagca
    23341 ttgcggcgaa tattcatctg gaaggtgata aaaaaccgat gcaagggcgg gcagatattc
    23401 aggctcgtcg gctgaaactg aaagaactga tgcccgatgt agaactgatg cagaagacgc
    23461 tgggggaaat gaacggtgac gcggaactac gcggtagcgg taactcggtg gcggcgcttt
    23521 taggcaacag taacggcaac ctgaaactgt tgatgaatga cgggctggtg agccgcaacc
    23581 tgatggagat tgtcgggctg aatgtcggta actacattgt cggtgcgata tttggtgacg
    23641 atgaggtgcg ggtgaactgt gcggcggcga atctggatat tgccaacggc gtggcgcgtc
    23701 cgcagatttt tgcttttgat actgagaatg cgttgatcaa tgttaccggc acggcaagtt
    23761 ttgcttcgga gcaactggat ttgactatcg atccagaaag taaagggatt cggattatca
    23821 cgctgcgttc gccgctgtat gtgcgtggga catttaaaaa tccgcaggct ggggtgaaag
    23881 ccgggccgtt gattgcccgt ggtgctgttg cggcggcact ggcaacgctg gttacgccag
    23941 cggcggcgtt actggcgctg atctcacctt ccgaagggga ggctaatcag tgccggacaa
    24001 ttttgtcgca gatgaagaag tgatgataat tgccggatgt ctgacatccg gcaattaaac
    24061 agatttagag tgttggcaaa gtgtgctttg ttcatgccgg atgcggcgtg aacgccttat
    24121 ccgtcctaca aatctttgcg aattcaatat attgtatgga aattcaggcc tgataagcgt
    24181 agcgcatcag gcttttcact cttattacaa cgattgatgt cgcgtctcat gggtcagcag
    24241 tagggcgatt aaggtcaagc cagccatcgc cgccagatat aaccccaccg cacctaaccc
    24301 gtagttagtc tgcaaccagg ctgcgatata cggcgcaacg gaagccccga gaatcgacgc
    24361 tacgttgtaa gagaatgatg ctccggtgta acgcacttct gtcgggaaca gctctggtaa
    24421 cagcgcgccc atcggcccga aggtcagccc catcagactt aaccccagca gcaggaaggc
    24481 aaaaaccaga atcgggttgc cagaaccgag cagcgggtta aaggcgaaca gcgcgaacag
    24541 gatgatcagc gtggtgatga ttaccatgct tttacggcga ccaaaggcat cagccagtaa
    24601 tccggcgact ggtaccatca cgccaaaacc aatcactgcc atcatcagca tccacaacac
    24661 ttcgttacgc ggcaggccaa gcccaactgg cgcggcggcg gtactaaagg tcatagagta
    24721 gaccgtcatg atgtaaaaca gcgtataggt tgccagcatg atgaaggtac cgagcaccgt
    24781 gacgcgtacg tgtttggtca gcagcgtacc cagcgggatc ttcacctgct ttttcgcttt
    24841 agcgactttc tcaaacaccg gcgactcatg cagcgacacg cgaacataca ggccgataat
    24901 gaccagcacc gccgagaaga taaacggcac acgccagccc cagctcataa actgctcgtc
    24961 ggtcagtagc caggaaagca gcaagaaagt gccgttggca aagaagaagc caatcggtgc
    25021 gcccagctgc gggaaggagc catacagtgc acgtttgcgc ggtggggcgt tttcagtcgc
    25081 cagcagcgcc gcgccgcccc attcaccgcc taagcccaga ccctgaccaa atcgagccag
    25141 cgccagcagc agcggggcga aaataccaat cgtggcatag cccggcagca gaccaatcac
    25201 cacggtcgaa atccccatcg ttagcaacga ggcgaccagc gtcgctttac gcccaacgcg
    25261 atcgccaaaa tgaccaaaaa cggcagagcc aatggggcgc gcgacgaagg cgatggcgaa
    25321 ggtggcgagc gactggagcg ttgctgccgt aggatcgccc tgcgggaaga agatatgcgg
    25381 aaacacaata acggccgcag tggcgtaaat gtaaaagtcg aaaaactcaa tggccgtgcc
    25441 aatgagagag gcgacaagga ctttattacg cgagttgatc ggcgtgtatt cttgctcgtg
    25501 gtcgagtgtt gtggctgttg cttgcataat cttttcttat tttttggcta acgaatagcc
    25561 atattacgca cagcaaaagt gatatttcaa tgtgtaacaa atcgcggaaa tggtcaaaaa
    25621 ccaggcaaaa aggggataat gtttaggcc
//
LOCUS       DACBVI010000059        24370 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.059, whole genome shotgun sequence.
ACCESSION   DACBVI010000059 DACBVI010000000
VERSION     DACBVI010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.059"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            106..1791
                     /locus_tag="IU325_11640"
     CDS             106..1791
                     /locus_tag="IU325_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HAP5809674.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(1966..2502)
                     /locus_tag="IU325_11645"
     CDS             complement(1966..2502)
                     /locus_tag="IU325_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP5809675.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            2586..3794
                     /locus_tag="IU325_11650"
     CDS             2586..3794
                     /locus_tag="IU325_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5809676.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            3794..4609
                     /gene="ybjI"
                     /locus_tag="IU325_11655"
     CDS             3794..4609
                     /gene="ybjI"
                     /locus_tag="IU325_11655"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HAP5809677.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFNQ"
     gene            4694..4978
                     /locus_tag="IU325_11660"
     CDS             4694..4978
                     /locus_tag="IU325_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809678.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(5019..6251)
                     /gene="mdfA"
                     /locus_tag="IU325_11665"
     CDS             complement(5019..6251)
                     /gene="mdfA"
                     /locus_tag="IU325_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HAP5809679.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLFVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     LLMVIFLKDKQMGNSHEG"
     gene            6536..7132
                     /gene="ybjG"
                     /locus_tag="IU325_11670"
     CDS             6536..7132
                     /gene="ybjG"
                     /locus_tag="IU325_11670"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP5809680.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            7190..7948
                     /gene="deoR"
                     /locus_tag="IU325_11675"
     CDS             7190..7948
                     /gene="deoR"
                     /locus_tag="IU325_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HAP5809681.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(7995..9197)
                     /gene="dacC"
                     /locus_tag="IU325_11680"
     CDS             complement(7995..9197)
                     /gene="dacC"
                     /locus_tag="IU325_11680"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAP5809682.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            9444..10070
                     /locus_tag="IU325_11685"
     CDS             9444..10070
                     /locus_tag="IU325_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HAP5809683.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEDRDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(10067..11182)
                     /locus_tag="IU325_11690"
     CDS             complement(10067..11182)
                     /locus_tag="IU325_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HAP5809684.1"
                     /translation="MHRQFFFSVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKMTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(11293..11676)
                     /gene="bssR"
                     /locus_tag="IU325_11695"
     CDS             complement(11293..11676)
                     /gene="bssR"
                     /locus_tag="IU325_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HAP5809685.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            11889..13214
                     /gene="rimO"
                     /locus_tag="IU325_11700"
     CDS             11889..13214
                     /gene="rimO"
                     /locus_tag="IU325_11700"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HAP5809686.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWRKICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            13440..13826
                     /locus_tag="IU325_11705"
     CDS             13440..13826
                     /locus_tag="IU325_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP5809687.1"
                     /translation="MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVI
                     KKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSGLSSKGTKEIEDDELAAYKKMAN
                     AFLAFSNKQIEDLIETGFLIEVKNER"
     gene            13816..14145
                     /locus_tag="IU325_11710"
     CDS             13816..14145
                     /locus_tag="IU325_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP5809688.1"
                     /translation="MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAK
                     VKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSKWERNEIKPSGPALRILNTLAIK
                     GPEVFAK"
     gene            complement(14215..15543)
                     /locus_tag="IU325_11715"
     CDS             complement(14215..15543)
                     /locus_tag="IU325_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAP5809689.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYGLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQIVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEFNAKNTIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGVSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSLYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECPTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(15551..17899)
                     /locus_tag="IU325_11720"
     CDS             complement(15551..17899)
                     /locus_tag="IU325_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAP5809690.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEEVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIEKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYLMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMINDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(18077..18988)
                     /gene="gsiD"
                     /locus_tag="IU325_11725"
     CDS             complement(18077..18988)
                     /gene="gsiD"
                     /locus_tag="IU325_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HAP5809691.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAIFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(18991..19911)
                     /gene="gsiC"
                     /locus_tag="IU325_11730"
     CDS             complement(18991..19911)
                     /gene="gsiC"
                     /locus_tag="IU325_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HAP5809692.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(19929..21467)
                     /gene="gsiB"
                     /locus_tag="IU325_11735"
     CDS             complement(19929..21467)
                     /gene="gsiB"
                     /locus_tag="IU325_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HAP5809693.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFNTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(21487..23358)
                     /gene="gsiA"
                     /locus_tag="IU325_11740"
     CDS             complement(21487..23358)
                     /gene="gsiA"
                     /locus_tag="IU325_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HAP5809694.1"
                     /translation="MPHSDELDAGDVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDQMLLRRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGSVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLTGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(23345..24310)
                     /gene="iaaA"
                     /locus_tag="IU325_11745"
     CDS             complement(23345..24310)
                     /gene="iaaA"
                     /locus_tag="IU325_11745"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP5809695.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLMAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
BASE COUNT         6171 a         6310 c         5790 g         6099 t
ORIGIN      
        1 gtttaggtgc gctgaatgaa tctgcgccct gaattctggt aaaaaacatt atcgtaaatt
       61 accatttctt tcaacagctt actagtaaac aagaagttag cctccgtgaa tataaacgtc
      121 gccgaattgt taaatgggaa ttacattctg ttattatttg tggtcctcgc gcttgggcta
      181 tgtctcggga aattacgact tggttcgatc caactgggta attccattgg cgttttagtc
      241 gtatcgctgt tattaggcca acaacatttc agcattaaca ccgacgcgct taatcttggc
      301 tttatgctgt ttattttctg cgtcggggtc gaagccggac cgaacttttt ttccattttt
      361 tttcgcgatg ggaaaaatta cctaatgtta gcactggtga tggttggcag tgcgctggtg
      421 atcgccttag ggttaggtaa gctgtttggc tgggatattg gcctgacggc cggtatgtta
      481 gcaggctcta tgacgtcgac accggttctg gtcggtgctg gcgatacact gcgtcattcc
      541 ggcatggaaa gcaggcagct ctcactggca ctggataatc tgagcctcgg gtatgcctta
      601 acctatttaa tcggtctggt gagtttgatt gttggtgcgc gttacttgcc gaaattgcag
      661 catcaggact tacagaccag cgcccagcaa atcgcccgcg aacgtggcct ggacacagat
      721 gccaaccgta aggtttattt gccggtgatc cgcgcctacc gcgtcggccc ggagctggta
      781 gcctggaccg acggcaaaaa tctgcgtgaa ctgggtattt atcgacaaac cggctgctac
      841 attgaacgta ttcggcgtaa cgggattctg gcaaatccag acggtgatgc cgtactacaa
      901 atgggcgatg aaatagcgtt ggtaggctat cctgacgccc acgcccgact cgatcccagt
      961 ttccgtaatg gcaaagaagt tttcgatcgt gaccttctcg acatgcgtat cgtcactgaa
     1021 gaagtggtcg ttaaaaacca taacgctgtt ggcaaacgcc tggcacaact gaagttgacc
     1081 gatcacggtt gcttccttaa ccgcgtcatt cgtagccaga ttgagatgcc gattgatgac
     1141 aacgtcgtgc ttaacaaagg tgacgtttta caagtcagcg gcgatgcccg ccgggtaaaa
     1201 accatcgccg atcgcatcgg ctttatctcg attcacagcc aggtcactga cttgctggca
     1261 ttctgcgcct tctttgttat tgggctgatg atcgggatga tcaccttcca gttcagcaca
     1321 ttcagtttcg gcatggggaa cgctgccggg ttgttattcg ccggaattat gctgggcttt
     1381 atgcgtgcta accacccgac cttcggttac attccgcagg gtgcattaag catggtgaag
     1441 gagttcggct tgatggtgtt tatggcaggc gttggtctga gcgctggtag cggtattaat
     1501 aacggcctgg gcgcgattgg cggtcagatg ttgattgccg gattgattgt cagtctggtg
     1561 ccagtggtta tctgcttctt gttcggtgct tatgtattgc gaatgaaccg cgcactgttg
     1621 ttcggcgcaa tgatgggcgc acgcacctgc gcgccggcaa tggagatcat cagtgataca
     1681 gctcgcagta atatcccggc gctgggctat gcgggcactt acgcaatcgc caacgtcctg
     1741 ctgacgctgg cagggacaat catcgtcatg gtatggccag gattaggata aaactgaagt
     1801 tgccctgaaa atgaaatttt tttgcacaac cgcagaactt ttccgcaggg catcagtctt
     1861 aattagtgcc actgcttttc tttgatgtcc ccattttgtg gagcccatca accccgccat
     1921 tttggttcaa ggttggtggg ttttttgttg cctaaaattt atctactacc ctgcaaccct
     1981 ctcaaccatc ctcaaaatct cctcgcgcga tagcggctta cggtcggtga caaaatgcag
     2041 cgtcatcccc tcaataaacg catcaagcgc gcgggcggtt ccgggttcaa accattgttc
     2101 gagcgtttgc tgactgcgct gcatccagtt ttgcattacc gtttttaata gcggttttcg
     2161 gctagccagc gcgtagagct ggtacatcag ctccatatta tccggcgttg caacctgtga
     2221 gctgtagatc atatcggtga tagcctggca tgcgcccgga gcatcactaa catcgctaaa
     2281 aaatgcctga tattgccggg acatgatctc agtaaaactg ctgaacgcct ccagcaacaa
     2341 ctcatcaatt cctgaaaaat agtaggtcat cgaccccaac ggtaccccgg caagggtagc
     2401 aattttgcgg tgcgtaacag catgtattcc gtaaagtttc accgcctcca gcgtggcctg
     2461 gataattttt tctcgccgtt gcggatcgtt agcgcgacgc atgtcatttc ctctctctgc
     2521 aattgtgtac aaatgtacac aaccttgcta atgttgtctt ccctcttctt attttgacgt
     2581 gatctatgac cgtaaattct tcacgtaatg cattgaaacg ccgaacctgg gcgctgttta
     2641 tgttcttctt tttgccgggc ctgttaatgg cgtcctgggc aacccgtacg cctgctatcc
     2701 gcgatattct ttctgtttcg atcgctgaaa tgggcggagt cctctttggt ctgtcgatcg
     2761 gttcaatgag cggtattctc tgctcggcgt ggttagtgaa acgctttggg acgcgtaatg
     2821 tcatcttggt cacgatgtcc tgcgcattga tcgggatgat gatactaagt ctggcactct
     2881 ggctgacatc gcccctgctc ttcgcggttg gtctcggcgt ctttggggca agttttggtt
     2941 ctgcggaagt ggcgataaac gttgaaggtg ctgccgttga gcgagaaatg aataagacgg
     3001 ttttgccgat gatgcacggt ttttatagcc tgggcacgct ggcaggcgct ggtgtcggga
     3061 tggcactaac ggcctttggc gttccggcaa cggtgcacat tttattggcg gcgctggtag
     3121 gcatcgcgcc tatttatatc gccattcagg caatccctga tggtacgggc aaaaatgctg
     3181 ccgatggcac ccagcatggt gaaaaaggcg taccttttta tcgcgatatc cagttgctac
     3241 tgattggtgt tgtggtgctg gcgatggcct ttgccgaagg ttctgccaac gactggttac
     3301 ccttattaat ggttgacggt cacggtttta gccctacttc cggctcgctg atttatgccg
     3361 gttttaccct ggggatgacc gttggacgct tcactggcgg ttggttcatc gaccgttaca
     3421 gtcgcgttgc cgtggttcgg gccagtgcac taatgggggc gttaggtatt gggctgatta
     3481 tttttgtcga tagcgcctgg gtcgctgggg tgtctgttgt actctgggga ctgggtgcct
     3541 cgctgggctt cccactgacc atttctgccg ccagcgatac cggccccgat gcaccaaccc
     3601 gcgtcagtgt ggtagcaacg accggttatc tggctttcct cgtcgggccg ccgctgctgg
     3661 gctatctcgg cgaacattat ggattacgta gtgcaatgct ggttgtactg gcgctggtta
     3721 ttctcgcggc tattgtcgcg aaagccgtcg ccaaacccga taccaaaacg cagacggcga
     3781 tggagaatag ttgatgagca ttaaattaat tgcggtagac atggatggta ctttcttaag
     3841 cgatcaaaaa acctataacc gtgagcggtt tatggctcag tatcagcaaa tgaaagcaca
     3901 aggcattcgc tttgtggtcg ccagcgggaa tcaatattat cagttgatct ctttcttccc
     3961 tgaaattgct aatgaaattg cctttgtggc tgaaaacggc ggctgggtag tgagcgaagg
     4021 caaagatgtt tttaatggcg agctgtcgaa ggatgcgttt gctactgtcg tggaacattt
     4081 gctgacgcgc ccggaagtgg aaattattgc ctgcggaaaa aatagcgcct ataccctcaa
     4141 aaaatatgac gatgccatga aaacggtggc ggaaatgtat tatcaccgtc tggaatacgt
     4201 cgataacttt gacaacttag aagatatctt ctttaagttt ggtctgaatc tttccgatga
     4261 actgattcca caagtacaaa aagcattaca tgaggccatc ggcgatatta tggtgccggt
     4321 ccacaccggc aacggcagca tagatctgat tatccccggc gtacataaag ccaatggcct
     4381 tcgccaactg cagaaattat ggggaataga cgacagcgaa gtagtggtct ttggcgatgg
     4441 cggtaacgat attgagatgc tgcgtcaggc aggctttagt tttgcaatgg aaaatgccgg
     4501 cagcgcggtc gtcgcagcgg caaaataccg ggcaggctcc aataaccgtg aaggcgtact
     4561 ggatgtgatc gataaagtgc ttaaacacga agcgccattt aaccaataaa acggcagcac
     4621 gcccggcaac acaatgcctg aatgaagatt gcataattcg cggaaattaa cccgctgcat
     4681 tatttcaggc gaaatgatta tgaaaaattg tctactgttg ggcgcgcttt taatgggctt
     4741 tactggtgtg gcgatggcgc aaagtgtcac cgtcgatgtg ccgagtggat acaaagtggt
     4801 tgtggttcct gattcagtca gcgttccaca agcggtcagc gtcgcgactg tgccgcaaac
     4861 cgtctatgtg gctcctgcgc cagctccggc ttaccgcccg catccttatg ttcgccatct
     4921 tgccagcgta ggtgaaggga tggttatcga acaccagatt gacgaccatc accattaatt
     4981 cgagaatgcc tgatcgcaca aagcaatcag gcattttttt acccttcgtg cgaatttccc
     5041 atctgtttat cttttaaaaa gataaccatc agcaacagcc acaaaattcc gttgacaagg
     5101 ttgaagagat taaacagtcc gttgcccccg ttcagccagg catgtttgct gatttcaata
     5161 ccgacggtaa agatcagcat ttgcagcatc cccatcgccg cagaaaccgt acctttactc
     5221 atatcgctgg caaacagagt taatcgcacc agtcccgcat tcgccagacc aataccgaaa
     5281 gcataaatac ttaacccggc ggtcatccat aaatacgcgt gcgatgagat aaccgttgcc
     5341 gcagcagcga caaacagacc aatcataatc ggccagccgc ccataataat cagcgaacgt
     5401 acggtgcggc gcgaggtcag acgcgctagc agcaagttac ccgcaattaa cgccccgaaa
     5461 ataggcactt gcagcaagcc atattcatag ctgctcaact gctcaccggt aatgatgata
     5521 atcggcgact gggcgatcca cgccagcaac ggcaggctaa cgaatcccag cgccagcgcc
     5581 cccgccacaa agcgaccgtt cttcagcacc agcttatagt cacgaccgag ttctttcagc
     5641 gacagtttct cgcctatacg cgtggcggtt tcaggcatgg ctcgttgcag accgaaaaag
     5701 gagatcgctg ccaatgcggc aaacaaaaca aacatcccct cccagggcag cacatggatc
     5761 cacgccgcgc ccaccagcgg accaagtagt ggagcaatca gcgccacgtt cgccattagc
     5821 gcggtgatct tgatacaaac cgcctcttcg aaggattcct gaattgcggc atatcccaca
     5881 gcgccaatga aacagaggct tatgccctgc aagaagcgca acagggtgaa ttgttcaatg
     5941 ttttgcgcca gtaatattgc cagacaggtg acgataaacc acaccactcc cgccagcatc
     6001 accggacggc ggccaatacg atccgacagt ggccccagga gccattgtaa aaacatcccg
     6061 cccgccagat acgcagtcat cgaagtagga acccaatcaa tgcccgcctg atattgttcc
     6121 accacggcca acataccggg ttgaatcata tcgttgccga tataggttga aaattcgtaa
     6181 agcaccagac agagagggaa aagtaacgcc tgacgtccaa gcctggcacc ggaagctaat
     6241 ttattttgca tgcaatttct tcgccaataa taatcgcgca aagtttaata aaagcgcagc
     6301 taacgagaaa gcgaattttg tagctgaaac cacggttaag cacattctta cattatcgcg
     6361 agtatagcta cgctttcttt aagttttatt taacctatgc ccgttacaat cacccaccgt
     6421 aaacaggccg cttgagggaa ataagacgat gccgctttac ccagtttaac ctgcacttta
     6481 ttctcaacga cttgcctgta ttggctccct tttaatcact ttgcgtcggg aagttatgct
     6541 ggaaaatttg aatctctctc tattctctct tattaacgcg acgccagact ctgcgccgtg
     6601 gatgatctcg ttggcgattt ttattgctaa agatttgatt accgtggtgc cgttgctggc
     6661 cgcggtactt tggctgtggg ggcttacagc acaacggcaa ctggtgataa aaatcgctat
     6721 cgcgctggcg gtcagcctgt ttgtgtcctg gacgatgggg catctttttc cgcacgaccg
     6781 accctttgtc gaaaatatcg gctataactt cctgcatcat gcggcggatg actcattccc
     6841 gagcgatcac ggtacggtga ttttcacctt tgcactggca tttttatgct ggcatcgcct
     6901 gtggtccggc tcacttttaa tggtgctggc cgtcgtcatt gcctggtcgc gcgtttatct
     6961 tggcgtccac tggccgctgg atatgctcgg tggattgctg gcaggtatga ttggctgcct
     7021 tagcgcccag attatctggc aagcgatggg gcataaactc tatcaacgtc tgcaatcgtg
     7081 gtatcgcttc tgttttgcat taccgatccg caaaggctgg gtgcgtgact gatctctgct
     7141 aaaaagtgta gtattgagcg gctcgcttca ataactattc agagggatta tggaaacacg
     7201 tcgcgaagag cgtatcgggc agctgctgca agaattaaaa cgcagcgata agttacatct
     7261 taaagacgcc gccgccctgc ttggggtttc ggagatgacg attcgtcgcg atctgaacaa
     7321 ccacagtgcg cccgtcgttt tgctcggcgg ctatattgtt ctggaaccgc gcagcgccag
     7381 ccattacctg ttaagcgatc aaaaatcccg cctggtggaa gaaaaacgcc gggcggcaaa
     7441 actggctgcg acgctggtag aacccgatca gaccctcttt tttgactgtg gcaccaccac
     7501 accgtggatt attgaagcga ttgataatga aatccctttc accgccgttt gttattcgct
     7561 aaataccttt ctggcactga aagagaaacc ccattgccgc gcgtttctgt gtggtggtga
     7621 atttcacgcc agcaacgcca ttttcaaacc catcgatttt cagcaaacgc tgaataattt
     7681 ttgcccggat atcgcttttt attccgcggc gggggtgcat gtcagtaaag gcgctacctg
     7741 ctttaatctt gaagagttgc cggtaaaaca ctgggccatg tcgatggcgc aaaagcatgt
     7801 gctggttgtc gaccacagta aatttggcaa ggtgcgtccg gcgcgcatgg gtgacctgaa
     7861 acgctttgat attgttgtga gcgattgttg cccggaagat gagtatgtga agtacgcgca
     7921 gacgcagcgt attaagttga tgtattaatg acgtataacc ggatgacgtt tcgcgccatc
     7981 cggttatcac aaaattaaga gaaccagctg ccgaaccact gatggaattt catcatcacg
     8041 aaatcccaca tccgaccaaa gaatccgccc tcttccacat tttccatcac gatcagcgga
     8101 cgctgctcaa tggatttacc gttaagctgg aaatcaatgg tcccgacaac ctgacctttt
     8161 ttcagcggtg cggtaagctg cggttccgtt aacgtgtagc tcgctttcag gtttttcagc
     8221 tgcccacgcg gtatggtcac tgagcccgct tcgcctgccc cgagattcac ttcgctctta
     8281 tctccaaacc agacgcgctg agtcacaaag gtggcatcag gtttaattgg cgtcacggtt
     8341 tcaaagaagc ggaaacccca ggtcaataat ttctcagact cattaaaacg gatacggtca
     8401 gttttcgccc ccagcactac ggagatcaaa cgcatatcgc cctgggtagc cgaagcaacc
     8461 agattatatc ccgcgcctgc cgtggttcct gtcttcatgc catcaacatt cagattgctg
     8521 ctccacagca gacggttacg gttaggctga cgaattttgt tgaaggtgaa ctctttctct
     8581 ttatgaatgg cgtactcttc cggcacatcg tggatcaatg ctttacccag caatgccata
     8641 tcgcgcgcgg tactgaactg ccccggcgca tccaggccgt gcaccgtctg gaaggtagtg
     8701 ttggtcagac ccagtttttt ggcataacca ttcatcagac caataaatga ctcctggctc
     8761 ccggcaacgt aatcagccag cgcaatacag gcgtcattac cggactggat aatcacacct
     8821 ttgttcaagt ctgccaccga aacctgatcg cccggtttga ggaacattac cgatgaacca
     8881 cgcagtgccg gatttcccgt cgcccaggca tctttaccga ccgtcaccat atcggtgagt
     8941 ttaatcttat cggccttaag cgcctgccca accacatagc tggtcatgat tttagtcagg
     9001 ctcgcggggt ccagtttttc atccgcattg ccttctgcca gcactttacc gctggcgtaa
     9061 tccattaaaa tccatgcacg cgcatccacg ctcggcgctt caacggtttg ttccgccgcg
     9121 aatgccgttg gggcaaaaag gaataaaaat gcagaacccg ctgcaagacc acgaaggaga
     9181 gaggagtatt gcgtcataaa tgccacccga gtatccattc aaaaaaaata cgttatatcg
     9241 ccgtatgata agttcggtga ttaataacgc acacacaaac ttaaagaaac cgtaagttgg
     9301 taaagttttt aaagtttatg caatatcctg ccgcttcgct gcttctggtg tcattttttt
     9361 gccagctgtt gcttaaaagt taactttact tcaccatcgg agtcctgttc agcctgtggc
     9421 tgcctgtcgg aaaaggggtg agtatgatta cgctgtgggg tcggaataat tcaaccaacg
     9481 taaaaaaagt attgctgacg ctcgaagaac tggaactacc ttatgagcaa attctcgcgg
     9541 gccgtgagtt tgggataaat cacgatgctg attttctggc gatgaaccct aacgggctgg
     9601 tgccgttgtt gcgtgacgac gaaagtgatc tcattctttg ggaatcaaac gccattgtcc
     9661 gctatctggc ggcacagtac gggcaaaaac gcctgtggat cgactcaccg gcacgtcgtg
     9721 cggaagcaga aaaatggatg gactgggcaa accagacgct cagtaatgct catcgcggga
     9781 tcctgatggg attagtcaga acaccaccgg aagatcgcga tcaggccgcc attgatgcca
     9841 gttgtaaaga gtgcgacgcc ctgtttgccc tgctcgatgc ggaactggca aaagtaaaat
     9901 ggttctccgg cgacgagttt ggtgtgggcg atatcgctat cgcaccgttt atctacaatt
     9961 tgtttaacgt tggcctgacc tggacaccgc gtccaaatct gcaacgctgg tatcagcaac
    10021 tcactgaacg ccccgcagtg cgcaaagtgg tgatgattcc cgttagctaa ttgcgtgggc
    10081 taactttaag taattcccca ctggactcgt cggtgagaac gtataaataa ccgtcgggtc
    10141 cagtgcgaac atcacgaatt cgctgccctc tgtccgttaa aatacggcca tcttctgtca
    10201 ctttgtcgcc gttgacgctc atcacaatga catctttatc tttcagcgcg ccaataaata
    10261 atttttgctg ccactggggg aatttatcgc tgttatagaa ggccatgccg ctcacggcgg
    10321 gcgaatcttt ccagtaaaaa acaggttgct cggtcccggc gacgatctcc cctttcgctt
    10381 ccggtatctt aaagcctgaa tagttgattc cccaggttgc cagcggccag ccgtagtttt
    10441 tgcctttttg cgggatatta atttcatcgc caccgcgcgg gccatgttca ttcagccata
    10501 gtgcattact ccacggattc atcgccattc cttgcggatt acgaatgcca taagaccaga
    10561 tctcggcgcg cgcaccggat tcctttataa aaggattatc atccgggatt tcgccctggt
    10621 cggtcagacg caccagtttg ccctgtaatt tatccagatc ctgcgccgtc gggcgctgat
    10681 tgttttcgcc cagagcaata aaaagataac ctttaccgtc gaataccagc cgcccgccaa
    10741 aatggttgcc ggtagacagt tttggcatct ggcgaaagac ggtgcggaag tcggtcatct
    10801 ttgagagatc atcacttaag cggccataac ccacagcagt tccggctttg ccatcatcgc
    10861 caacttcgga ataacttaac cagatgcggc gagactgagc aaaatcaggc gctaaaacca
    10921 cgtccagcag gccgccctgc ccgtgcgccc aaacgtccgg aactccggaa agcggcgcag
    10981 ataatccttt tcctgcttgc cagtgacgca actcgccgcc gcgcagagtg attaacatac
    11041 cgtgattgtc gggtaaaaag gccagcgccc agggatggtc gagtttgtct tgcagtactt
    11101 cgacatttac cgttgcagga gccgcccaga gagcggaaga aagacaaata aggggcacag
    11161 agaaaaagaa ttgtcgatgc ataacgcctc cggattgact ctggcttaaa gcgtagtcag
    11221 tggaggagat aacaaaatca tttttacaaa aacttaaaca tgaaggggga gacgctttct
    11281 cccccttagt tttcaggcct tctcaagcat ggcgtgcttc tgcaggctct ggatactcag
    11341 cgttaagctc atcagacaat tttcaagctt atcggcgttg acggtaataa cagtcgggca
    11401 atcatggtgc ccactcatca aacatactgc ggctgtcgct aatgcttctt cagcatgatg
    11461 aagagcactc cactcttcct gatccagatg aagattcaac cgcagcgatt tatcgtgcag
    11521 ttcgcgattc agtttaaaaa agttatctcg tagatgattg ctttcgctga cggacatgta
    11581 tccttttgcc ttcctcagtt gcacgtatgc ggcgaggtca acgcgtgcgt cgatcaaccg
    11641 gttcagcaga tcgattcgct gtctgtcaac gaacatactt cgatcctcct ctttccagcc
    11701 ttgttgcaat taaatagtat ggctattttt tgagcaaaat gctgtccagg atcaattctt
    11761 cacgccgaat taacaaaatt tacatttcct tcgcctccgc cagcgaatga gattgcgata
    11821 caaattttat ccagaactct gtagaatccc tgccctgacc tttcctgtaa ctgaatattt
    11881 tttaagctat gagcaaagta actccccagc cgaaaatcgg ctttgtttcc cttggctgtc
    11941 cgaaaaacct tgtcgattca gagcgtattc tcaccgaact acgcactgaa ggttatgacg
    12001 tggtaccgag ctatgacgat gcggacatgg tgatcgtcaa cacctgcggc tttattgaca
    12061 gcgcggtaca agaatcactg gaagccattg gtgaagcgtt gaatgaaaac ggcaaggtaa
    12121 ttgtgaccgg ttgtctgggg gcaaaagaag atcagatccg cgaagtccac ccgaaagtgc
    12181 tggaaatcac cgggcctcat agctatgagc aggttctgga gcacgttcat cactacgtgc
    12241 caaaaccgaa acacaaccca ttcctgagcc tggtgccaga acaaggcgtg aaactgacgc
    12301 cgcgtcatta tgcctatctg aaaatttctg aaggctgtaa tcaccgctgc accttctgca
    12361 ttattccgtc tatgcgcggc gacctggtga gccgtccgat tggcgaagtg ttaagtgaag
    12421 cgaaacgtct ggtagatgcg ggcgttaaag agattctggt gatctcgcag gatacttccg
    12481 cctatggcgt cgatgttaaa catcgtactg gcttccacaa cggcgagccg gtaaaaacca
    12541 gcatggtcag cctgtgcgaa cagttatcga aactggggat ctggacacgt ctgcactacg
    12601 tttaccctta tccgcatgtg gacgacgtca tcccactgat ggcagagggc aaaatcctgc
    12661 cctatctgga cattccgttg cagcacgcca gcccgcgcat tctcaaactg atgaaacgtc
    12721 cgggttctgt agatcgccaa ctggcgcgca tcaaacagtg gcgcaaaatc tgcccggaac
    12781 tgaccctacg ctcaaccttt attgttggct tccctggcga gacggaagaa gatttccaga
    12841 tgctactcga cttcctgaaa gaagcacgtc tggatcgcgt tggctgcttt aaatacagcc
    12901 cggttgaagg tgcagacgcc aatgccctgc ctgaccaggt tccggaagaa gtgaaagaag
    12961 aacgctggaa ccgtttcatg cagctgcagc agcagatttc cgccgagcgc ctgcaagaga
    13021 aagtgggccg tgaaattctg gtgattatcg acgaagtgga cgaagaaggc gcgattggtc
    13081 gcagcatggc agatgcaccg gaaatcgacg gcgcggttta tctcaatggt gaaaccaacg
    13141 ttaagccggg tgatatcctg cgtgtgaaag tcgagcacgc cgatgagtac gatttgtggg
    13201 gtagccgggt ttaattaatc ttaaggcaga tctaacaagg atattgttct tcgatctgcc
    13261 tctgctaccc ttcccgatgt cctaccctta cgcacaaaat cccctgacgc ataaatcgag
    13321 aaaaatgaat cgtactcgag gcaactctca ctttttcaca ccaaattaaa gttttgatcg
    13381 caattacttg aaataagcgt aatggttact atagtaaaca ttaccacaag gatggatgta
    13441 tgggtgtgta taaggcaaga cgattcagcc aaagcacgaa aaaacttggt attcacgata
    13501 aagttctcat ggctgccgct gaagaagtga tgcagggcat atgggaggct gatttaggta
    13561 gcggtgtcat caaaaaacgt ttacccctcc aacaaggaaa gagtggtgga gccagaacaa
    13621 ttatcttttt taaatcagca aatcatgtgt ttttttatga tggttggtcc aagagtggtc
    13681 taagcagtaa aggaaccaaa gaaatcgaag atgatgaact ggcggcctac aaaaaaatgg
    13741 caaatgcatt tctggccttt agcaataagc aaatagagga tttgatcgaa actggctttt
    13801 tgattgaggt gaaaaatgag cgataaatta caaatgatgc tggaagatgc aaaggaactg
    13861 aatgcactgg gcctgatgtc tgatagtgaa gttgatgcca ttgccgtcat ggtcaaagct
    13921 cgtgaagtca gcgcccgtat agccaaagtc aaatcaatga cgggtgaaga gatcaaagcc
    13981 atccgcaccc gatacggcat gtcacaatcc gtgctggcac atacaatggg aatgtcaaaa
    14041 gaaagtgtct cgaaatggga acgaaatgaa ataaaaccga gcggcccggc actacgtatt
    14101 ttgaatacat tggcaataaa agggccggaa gtttttgcaa agtaaaatgt ttgcaaatga
    14161 tttaccacat tcattagtac cccttgatta aggggtacta acactattct aattctactg
    14221 ccagctaatc tgtgccctgc cgtttttctt actcttatac agtcgctcat ccgccagcat
    14281 tttcaagcgt tcaacggtag gacattcatt tgaatgcgct acaccgatac tggtgcttaa
    14341 attaatgctt cccacctgga aatgaacaat ttcttttaga atcgatagca actcttccag
    14401 cgttccaccc gtataaatca ccgcaaattc gtcaccgccg aagcggtaga ctttaccttt
    14461 atctaccggg atctgtgatt taaggatttt caccactttc atcaacactt catcgcccag
    14521 caaatgaccg aaagtgtcgt tgatgttttt gaatttatcg acgtcaaaca ggcacaaata
    14581 tccgcctttt tccggcaggg cttttaaatc ttcgtcaaag gattttctgc ccatcgcttt
    14641 ggtcaggtca tccaggaacg ccgagttata cagactgtag agttttttga catcggcagc
    14701 tgaaaagaaa agcccgccat ccagtacgcg ttcaaaatgg ttattaatga tggcttcttt
    14761 ctccacaata ccacgattaa tcactctgcg taccagatag aggcaactga cacccgatat
    14821 cactgccacc aatgcggcaa agaaaataat caatgtacag acccgcaaat aaacatcttt
    14881 tttatacgca aatacgtaca atccggtcgg caaataggcg aagtgctcca tcgcttccga
    14941 aacggtgaag atggtgtttt tagcattaaa ttcatcaccg gtcaccggtt taccgttctt
    15001 aaacagagag aacatcaggt tctcgctatc catcgccagc gattttgaca gtaaagaaaa
    15061 acgctccaga ccaagcaaaa atatcagact acgattatga gttaccggat agaggtaata
    15121 gacgaaggtt ttaccgtcaa aagagataaa ccctgtattt ttgacatgtc gatacttcac
    15181 aatctgcata atatctgcac aataattttt ctcgaactcc tttacctgaa tacattcgtc
    15241 actggaaatc agataaggac cgacatcgta caaataagag ggtgaaaccg agtcggcgta
    15301 agctgtgcgc aagccaacca acagctgttc ctccgtggga tttagcgtgt tgataagatc
    15361 aattgccacg ctacgccgat attcagcaat atttgccacc gcaaaagcag tatcaataac
    15421 ggactgtttc aggccataca cccgttcctt taccatttga gtgtagatcc cgcaggcaac
    15481 tgctgaaatc atgataaaaa tcagcagact gactgtaagt acgaacttat tgattctgga
    15541 catacgtaaa ctactctttt actaattttc cacttttatc ccaggcggag aacggcatgg
    15601 gtttctggta cagatacccc tgagcctgaa cacatcctaa tttgattaat tcgcgcgcaa
    15661 cgtcttcgct ctctacgcct tccgcaatca ccgggatccg gttatagcgt gccagatcga
    15721 aaataatgcg cacaattttt cccgcagtac cgaaaatatc gccggtaaat tgtgagtcca
    15781 cctttatgct atctggatta atttcataaa aacgtttcag gtttgacaaa ccggtgccaa
    15841 agtcatcaat cgatatccgt accccttgtt tacgcagttg ttcaatattg agataaaaat
    15901 gttcattaat tcgctcgatc acggtttcag tgatctcaac acagaggctg ttcgccttca
    15961 ggccattttc tttcatgatg gtggtaaaac gctcatgaaa gtgtggttcg tttaactgcc
    16021 agggcgaaac attaatatgc agtaagaaat catccttcag cgacgcacgc tgactaaaat
    16081 gtctgaattc acccattgcc gttttaataa tctgataacc cagctcattg ataaacccga
    16141 tatcttcagc aatggtaatg aactttagcg gtgaaatgat cccccgctcc gcagacaccc
    16201 aacggcacaa cgcctccgcg cccagaatgg cgcgggtgtt aatatcaacg atcggttgat
    16261 agacgaggaa tagttcgcca atttccattg catggcgaat attacgcgca atccagatat
    16321 tctcttctat ttcgttgagt ttcgcgtcat cacagaaaat aaactctgag aacatatggt
    16381 cacggatact ggaaatcgcc agtttcagtt tttctgcgta actccaggcg ttttctccct
    16441 gcaaaggttc acaaatggca gacttatgga tgcgcagatt gcggtagcca aagctgctgt
    16501 caaaatcgtt tatcatcgct attttacggg taaatgtctg tacattttcc cggcacagaa
    16561 gtaaatagag atcggcattg aatgtcacga tatcgcagca ttgcgcatat ttctcggcaa
    16621 ttcgctgaca caatttcctg acgattttat tgcgttcatc aacaccaaac accgcttcca
    16681 cggcttccag accacacagt ttgatgatcc ccacagccag atactgatta ttgttggact
    16741 caataatcgc catattttta ataaaaccgc tttcctgatc gtaagagatc agtgagagca
    16801 atttatttga ccgttcaatt tcggcatctt tcatttccgc gatcgaattt ttgatgctga
    16861 taatctcttt cgcatcgcct tcgcgaattt tcagagcctc gatctgatcc gttgagtcgg
    16921 gcaaaaacgc cagcgtatta accagctcgg tcatttcaga aaattgccga cggaacacac
    16981 gagtggtcat taaataaacc accagcaata cgataataat gaaagcaaac ggcagataaa
    17041 aaagtttatc tgatgcgttc aataaattac cgccaggata ataaaaatag atttcgaata
    17101 atgcgttgtt tggcaccgct gatgtttttt cgatatattc agtatcgaca gaatcaataa
    17161 tattataatt accttcgcta ttataaataa tcagtttttc acgcgcgagt tgattcttgg
    17221 caatgatctc atggccttgt ttcaaaaata gaacttcatc attagcgctg atttgatcaa
    17281 gatactcgtt cagatatcgc cagtttaaat cgacatgtat tttatccacc gctaacaaat
    17341 cgtgatcttt gccgttgatg cgtagcgtaa aagagataca ttttttaccc ggacgatcaa
    17401 agcaattgta ggccggattc cagctgttgt tagagtcatc tgaagccacc gaccatggtc
    17461 gtttcgatgg attaaaatcg gcaaaaataa cccgccctga ctcgtcgaca agcggtccat
    17521 ttacttcttc ctgatgataa acaacgattt tatcattcgc cctccgcgaa aagagataat
    17581 atttattatc atcaagtacc agtcccaccg agtctaaaaa tggcatcatt tccattttaa
    17641 ggatcacagc gcgttgaatc tcctctgtat tttttaataa atacagtgag tcctttaatg
    17701 tcagggccga acgttcaata tctttaaaga aatagccaac gtttttctga ataagttcaa
    17761 agcggctatc ggcacgtttt accgcgcgtt ccgataccag ttgatagtta atgatgaaaa
    17821 aaaggccaat aaatgacagt gctgccaata aaaataaaat tatcagcctt atctttatct
    17881 tttcgtataa actcagcatg ttaatacatt taacaaaata actgtctgaa cgctgtgccg
    17941 ctgcaaaaac agtcccttgc tgcggctttt taaccaaatt acccgattct acaaaatcag
    18001 cagaaagtta caacacttgc ttagggcaat aatttcctgt gaggtgatta ccctttcaag
    18061 caatattcaa acgtaactat cctttaattt tcggatccag cgcatcgcgt aaaccatcac
    18121 ccaacaaatt gaacgccagt acagtcagaa aaatagccag ggccggaaaa atagcgacat
    18181 gcggcgcgat aaccatatcc gctcgggcct cattgagcat tgcaccccac tctggtgtcg
    18241 gcggctgcgc accgaggccg agaaatgaca ggctggcggc agagataatc gaggtaccga
    18301 tgcgcatggt gaaaaacacc acgatagaag agacagtccc gggcaagata tggcgcaata
    18361 agatggtcat gtcgctggca ccaatactgc gcgccgattc aataaaagtt tgctgtttca
    18421 acaccagcgt gttgccacgc accagacggg caaaagccgg aatggaaaaa atggcgactg
    18481 caataatcac gttagcaatg ccgcttccca acaccgcaac aacagcgatc gccagtaaaa
    18541 tacccgggaa ggcaaacagc acatcgcaaa tgcgcatgat cagccgatcc caccagcctt
    18601 cataatatcc agccagcaaa cccagcaacg tcccgatcgc cgcaccgata aacacggcaa
    18661 acacgcccgc cgccagcgag atttgcgcac caaccaggac acggctgaaa atgtcacgcc
    18721 ccagtgaatc gacgccaaac cagtgctgca aagaaggtcc gttattcaga ttgtcataat
    18781 caaaataatt ttcggcgtca tagggagcga tccaacgtgc aaaaatggcc accacaatca
    18841 ataaaataac gaataatgcg gcggtcatcg ccatatgctg acggcgaaat cgtcgccaga
    18901 attcatgcca cggtgtacgt acctggtcag gtttgaccag tggcatggcg tttaacaccg
    18961 cctgacgtcg ccagttaaat agtcgcatcc ttacttgtac ctgatagccg ggttaatggc
    19021 ggcgtaaagc acatccacca ctaagttgat aagaataaat tccagcgaga aaagcagaat
    19081 ttccgcctga atcaccgggt aatcgcgcat ttctacggag tcaacgagta agcgtccaag
    19141 tccgggccag ttgaaaactt tctccaccac gatagaacca ccgagcaaaa agccaaactg
    19201 taagcccatc atggtcacaa ccgggatcat cgcgttacgt agcccgtgtt tgaggacaac
    19261 ccaggtttcg ctcacccctt tcgccctcgc ggtacgcata taatcttcgc ttaaaacatc
    19321 gacaaacgac gcgcgggtaa agcgcgccat cacggctgcc actgccgcgc cgagcgtcag
    19381 ggagggtaaa atgtagtgct gccaactgtc tgctcccacg gtaggcagcc agcccagttc
    19441 aacggagaat acctgaatta aaagcatccc cagagcaaat gccggaaacg agatccccga
    19501 caccgcaatg gtcatactca atcgatccgg ccaacgatta cgccagacgg cggcgataat
    19561 tcccgccgcc ataccaaata tcaccgccca gaccatactg gttatggtca gccacagcgt
    19621 tggcataaag cggctggcaa tctcatcggc aaccggacga cgcgacacca tcgacaggcc
    19681 aaaatccccc tgcacagcat tgctgatata gtgccagaac tggtgataca gcggctgatc
    19741 caaccccagc tgctgacgca ccagttctat aacctgcgca tcagcttcgg gcccggcaat
    19801 caatcgcgcc ggatcgccgg gcagcatatg cacaaataaa aacaccagca ccgagacgat
    19861 aaacagcgtt ggaatcaacc ccagtaagcg tttgataacg taattaagca ttccactccc
    19921 tgcgttgctt attgcaaatc cgcgtcttca aagctgaaac cggtgtctgg catgatccaa
    19981 aaaccggtca ggtttttact gtgtgccgac accagttttt ctaccaccag cgggatccac
    20041 ggcgactctt gccagatgat atcctgcgcc gccttatata aacgggtctt ttctgccgga
    20101 tcattagttt tcagtgcctg agccaggaag tcatccacct gtttattgct gtaaaacgcg
    20161 gtattaaaca gcgtcggtgg ccagttctgc gaggcaaaca gcggcgatag tgcccagtcc
    20221 gcttcgccgg ttgaagccga ccagccagtg tagaacatcc gcacgccgct ctctttttgc
    20281 cctttacctt caacttctgc cgcccgctgt ccggcatcca tcgcagtcac ctgggcttta
    20341 atcccgacct gcgctaactg ctgctgggta aattgcagca ctttctgcgc ggtgctgtgg
    20401 ttatgtgacg accacagcgt ggtattgaaa ccgttgggat atcccgcctc tttcagtaat
    20461 tcgcgcgctt tcactggatc gtaaggccac ggtttataac tttgcgcgta ggcgatactc
    20521 ggcggtacca caccagtagc tggcgttgca tagcccgcga aggccacttt caccagcgcc
    20581 gggcggttaa tggcgtaatt cagcgcctca cggaccttcg ggttatcgaa cggcttttgc
    20641 gtcacgttca tactgatata acgctgcata attgacggac tggccatcaa ctcgatattt
    20701 ttgtttttct ccagcagtgc ggcctgctcg tagggaatgg ggaaagcaaa ctgcgcttca
    20761 ccggtttgca gcattgccgc gcgggtgttg ttatccgcca ccggacgcca ggttatgctg
    20821 tccagtttgg gcaatcctgg ctgccagtaa cccgcgaatt ttttcacctt cacaaaatcg
    20881 gtctgattcc aggtatccag ttcatacggc ccggttccca ccggatgaaa accaatctcc
    20941 ttgccatatt tttccagcgc tgccggcgaa atcatcgcgg tcgccggatg ggcaagaata
    21001 ttaataaacg ctgagaacgg ctgtttgagg gtaatcttta ccgttgttgg atcgatcgct
    21061 tccgttttgg caatattctt atacaggttg tagcgtttaa gatgattcgc cggatcgctg
    21121 gcccggtcca gattcgcttt taccgccgct gcgttgaaat cggtgccatc ctggaattta
    21181 attccttccc gcaatttcac ggtgtatgta aggccgtcat cggaaacggt ataactctcc
    21241 gccagcacgt ttttcagttt catctcttta tccagaccaa acagcccctg gtaaaacgat
    21301 ttcgctacgg cctgagataa cgtgtcattt gcgtcatacg gatcgagcgt ggtgaagttc
    21361 gatcctaccg ccaccaccac atctttggca gcgaatgcac aagatgccat caacgctgtc
    21421 gcaatgccca gcgccactaa cccactacgg tgtacagctc ttgccatttt attctccgcc
    21481 tgaatgttat ctacgcatga atgcgtattc tgattgtggt tgtgcgacgt aatgccccgg
    21541 cccgacgcat tgcaacgaga cggctgccac ctcttcgcca cgcagatgaa tattgctggg
    21601 aagatcgtcc gacagcagta cacgctgcgg tcgttgtcgg gacggttcag cgaccggaac
    21661 tgccgccagt aatttacgcg tataaggatg ctgcgggttt tcgaagaccg cgcgccgtgg
    21721 accaatttca acaatttgtc cgagatacat caccgccaca cgatgactaa tccgctctac
    21781 cacggccata tcgtgggaga taaacagata cgcaatgccg aaatcacgct ggagatcgag
    21841 caacaagttg ataatctgcc cgcgaataga aacatccagc gccgagacgg cttcgtcggc
    21901 aatgatcact tttggattca atgccaacgc gcgagcaatg cagatgcgct ggcgctgacc
    21961 gccggaaaac tcatgcgggt aacgccaggc atgttcaggt aacaggccca cgcgctccag
    22021 caaccacgca acgcgtgcag ccgcttcttt acctgtcaat aaaccgtgta cgcgcagcgg
    22081 ttcgataatc gaatcaccga tggtctgacg tgggtccagc gaagcgtaag ggtcctgaaa
    22141 aataaactga atatcccggc gcaatgcctg aagtttgccg ggtgacaagg tatcgattcg
    22201 ctgaccgtta aagataattt cgccgccctg cgattcgacc aggcgcagca acgcccgccc
    22261 ggtagtggat ttaccgctgc cagactcgcc caccagcgat agcgtttcgc caggccagag
    22321 atcaaaactg actttctcaa cggcatgcac ttcccgcgtt acgcgattca acaaaccgct
    22381 gcgcaaaggg aaacgggtga ccagattacg cacccgtaaa acaggttcgc catccaccac
    22441 cgttttctgc tcgatgggtg gttcctgttt cgctggatgt tcaagcgata tcaatgggaa
    22501 acgtcgggga taatctaacc ctttcatcgc accaagttgc ggaacagcag ctaacagcgc
    22561 acgggtgtaa ggatgttgcg gtgcatgaaa aatctgttcg acgctacccg tttccaccgc
    22621 ctcgccctga tacatcacca gtacccgatc ggcaatctct gccaccacgc ccatatcgtg
    22681 agtgataaag ataacgccca tcgacatctc tttttgcaat actttgatta attgcaggat
    22741 ctgcgcctga atagtgacat ccagcgcggt ggttggctca tcggcaatca gcaccgccgg
    22801 gcggcatgac agcgccatcg caatcatcac tcgctggcgc atcccgccag agagttgatg
    22861 cggataacgt gaaagaatgg tttgtgcctc cggaatgcgt acctgatcca gcatccgctt
    22921 cgcctcgacc atcgcttctt cacgactggc gttctgatgc agacgaattg attcggcaat
    22981 ctgttcaccc acagtaaata ccgggttcag cgatgtcatc ggttcctgaa atatcatcgc
    23041 catatccgca ccgcgcacat ggcgcatttg tgcagcgctc tgctcgctaa gttcaatcac
    23101 ttcgcgactg cgccgccgca acagcatttg atcgcactgc actaaaccgc ccgcctgttc
    23161 caacaagcgc atcaatgcca gcgcagtcac tgacttacca gagccggatt cgccaacaat
    23221 tgccagcgtc tcaccgcgtt gcaggctaaa agagagattg cggaccgcag ctattttctg
    23281 ctggtcctgc ataaaggcaa tattcagatt ttcaaccgcc agcacatcac cggcatcaag
    23341 ttcatcactg tgtggcaacg gtgtcccctt tttcacggta gataccggtg gttggcgtat
    23401 cgcctgcgta gccccaggcg cgatacattc cttcggtgtt aaacggtagc gcgacattgc
    23461 cttcatggtc gatagcgatt aagccaccgc taccgccaag cgcagggagt ttttccatta
    23521 ctacccgctc gcaggcttcc gcgagactta atccgccgta atccattaac gcggcgatgt
    23581 catatgccgc cagcgcgcgg atgaagactt cgcccgtgcc ggtacatgaa accgccacac
    23641 tggcgttatt ggcgtagcat ccggcaccca ctaagggact atcgccaact cgtccgggta
    23701 atttattggt cattccgccc gtggacgtgg ctgccgccag attgccgtct aaatccaacg
    23761 ccacggcccc cacggtgccc attttctgtt tttcatccag tggcgcaccg ctatggtcga
    23821 ggactgttgc cccttcctca cgcgctgcca ttagttgttc ataacgcaaa ggcgtggaga
    23881 aaatctccgg tgagacgcgc tccatgccat gagcaaacgc aaaattttct gccccttcgc
    23941 caatcatcat cacatgcggg ctttgctcca tcaccagccg ggcggcaaga accggattac
    24001 gcagatgact aacgcccgcc accgcaccgg ctttcagggt gttaccatcc atcacacagg
    24061 cgtccagttc atgggtttca tcacgcgtaa agacagcgcc aattccggcg ttaaacagtg
    24121 gacactcttc cagcagacgc accgcttccg tcaccacatc cagcgcactt tcgcccgctt
    24181 ccagcatttt ctgcccggtt tcaacaatgg cagacagcgc ctcgatgtag cgtaattcct
    24241 gttgcagact catctgcgcg cggctaattg cacctgcgcc gccatgaatt gcaatgactg
    24301 ctttgcccat agtattcatc cattaagggt ggctcgccac ttttgctata aatatcagaa
    24361 tttttatgaa
//
LOCUS       DACBVI010000060        24038 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.060, whole genome shotgun sequence.
ACCESSION   DACBVI010000060 DACBVI010000000
VERSION     DACBVI010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.060"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            150..1493
                     /locus_tag="IU325_11750"
     CDS             150..1493
                     /locus_tag="IU325_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1338 family protein"
                     /protein_id="HAP5809696.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHSGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            1718..3373
                     /locus_tag="IU325_11755"
     CDS             1718..3373
                     /locus_tag="IU325_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HAP5809697.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            3513..3737
                     /locus_tag="IU325_11760"
     CDS             3513..3737
                     /locus_tag="IU325_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HAP5809698.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            3800..4339
                     /gene="rimL"
                     /locus_tag="IU325_11765"
     CDS             3800..4339
                     /gene="rimL"
                     /locus_tag="IU325_11765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HAP5809699.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNVQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(4331..5311)
                     /locus_tag="IU325_11770"
     CDS             complement(4331..5311)
                     /locus_tag="IU325_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809700.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQDAYISDSVTIRDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIEKHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            5435..6427
                     /gene="tehA"
                     /locus_tag="IU325_11775"
     CDS             5435..6427
                     /gene="tehA"
                     /locus_tag="IU325_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HAP5809701.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            6424..7017
                     /gene="tehB"
                     /locus_tag="IU325_11780"
     CDS             6424..7017
                     /gene="tehB"
                     /locus_tag="IU325_11780"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HAP5809702.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSVANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            7320..7988
                     /locus_tag="IU325_11785"
     CDS             7320..7988
                     /locus_tag="IU325_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HAP5809703.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPHSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(8023..9234)
                     /gene="ydcO"
                     /locus_tag="IU325_11790"
     CDS             complement(8023..9234)
                     /gene="ydcO"
                     /locus_tag="IU325_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HAP5809704.1"
                     /translation="MLRYLYVITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATRAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FAFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALFFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITGMMSALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLFGIGSAFWGLIAGGVCY
                     VVLNLIANRNR"
     gene            9287..9823
                     /locus_tag="IU325_11795"
     CDS             9287..9823
                     /locus_tag="IU325_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP5809705.1"
                     /translation="MENLARFLSTTLKQLRQQHGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQSLNCGKGARFAADVTHVYR
                     NGGEQIVHFHSLIHYPRS"
     gene            9896..11857
                     /gene="rlhA"
                     /locus_tag="IU325_11800"
     CDS             9896..11857
                     /gene="rlhA"
                     /locus_tag="IU325_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HAP5809706.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDFYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVSEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPAELHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTIYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLAGY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(11949..12179)
                     /locus_tag="IU325_11805"
     CDS             complement(11949..12179)
                     /locus_tag="IU325_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2554 family protein"
                     /protein_id="HAP5809707.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQQSRKPEAGQR"
     gene            12401..12577
                     /gene="hicA"
                     /locus_tag="IU325_11810"
     CDS             12401..12577
                     /gene="hicA"
                     /locus_tag="IU325_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HAP5809708.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIK
                     EPLRKAILKQLGLS"
     gene            12623..13039
                     /gene="hicB"
                     /locus_tag="IU325_11815"
     CDS             12623..13039
                     /gene="hicB"
                     /locus_tag="IU325_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HAP5809709.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            13118..14524
                     /locus_tag="IU325_11820"
     CDS             13118..14524
                     /locus_tag="IU325_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP5809710.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLKALELALQEYPVKACWLMTNSQNPLGFTLTPQKKARLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPAPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            14769..15914
                     /locus_tag="IU325_11825"
     CDS             14769..15914
                     /locus_tag="IU325_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP5809711.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVSGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATLFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            15932..16945
                     /locus_tag="IU325_11830"
     CDS             15932..16945
                     /locus_tag="IU325_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP5809712.1"
                     /translation="MTYAVEFGNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQTNGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            16946..17887
                     /locus_tag="IU325_11835"
     CDS             16946..17887
                     /locus_tag="IU325_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP5809713.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            17877..18671
                     /locus_tag="IU325_11840"
     CDS             17877..18671
                     /locus_tag="IU325_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP5809714.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            18693..20117
                     /gene="patD"
                     /locus_tag="IU325_11845"
     CDS             18693..20117
                     /gene="patD"
                     /locus_tag="IU325_11845"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HAP5809715.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(20214..20309)
                     /gene="yncL"
                     /locus_tag="IU325_11850"
     CDS             complement(20214..20309)
                     /gene="yncL"
                     /locus_tag="IU325_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HAP5809716.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            20504..20677
                     /locus_tag="IU325_11855"
     CDS             20504..20677
                     /locus_tag="IU325_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HAP5809717.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            20763..20996
                     /gene="ydcY"
                     /locus_tag="IU325_11860"
     CDS             20763..20996
                     /gene="ydcY"
                     /locus_tag="IU325_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 domain-containing protein"
                     /protein_id="HAP5809718.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(20997..21446)
                     /locus_tag="IU325_11865"
     CDS             complement(20997..21446)
                     /locus_tag="IU325_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA-like transporter family protein"
                     /protein_id="HAP5809719.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAA
                     LVASQLIGGLVLDIFRSHGVPLRALIGPICGAILLVVGAWLVARRTF"
     gene            complement(21443..21961)
                     /locus_tag="IU325_11870"
     CDS             complement(21443..21961)
                     /locus_tag="IU325_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HAP5809720.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTLAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            22142..23179
                     /gene="curA"
                     /locus_tag="IU325_11875"
     CDS             22142..23179
                     /gene="curA"
                     /locus_tag="IU325_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HAP5809721.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVQGFDVCLDHHADDFAEQLAKACPKG
                     VDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            23377..>24038
                     /locus_tag="IU325_11880"
     CDS             23377..>24038
                     /locus_tag="IU325_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP5809722.1"
                     /translation="MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVTLAVE
                     NLTSQDLAELEELLEKLQQAQEKGDMEQIINANRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELRERIENYRLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT         6057 a         5774 c         6222 g         5985 t
ORIGIN      
        1 aaaatattat taacaattaa agcaattatg ttacagcaaa atggataata ttgatgtttt
       61 cgcggcgaga tcacagtttg taaattcttc ccgccggagt gaatgcggtt acctacactc
      121 cagattactg accactggag gcagacacta tggcgaacag catcacggcg gatgagattc
      181 gggaacagtt ttcgcaggca atgtcagcca tgtaccagca agaagttccg cagtacggca
      241 cgctgctgga actggtagct gatgtgaatc tggctgtgct ggaaaacaat cctcaactgc
      301 acgaaaaaat ggtaaatgca gacgagctgg cgcgactgaa tgttgaacgt catggggcga
      361 ttcgtgttgg gactgcacaa gagcttgcta ctcttcggcg gatgtttgcc attatgggga
      421 tgtacccggt gagctattac gatctctcgc aggcgggggt gccggtacat tcgacagcat
      481 ttcggcccat tgatgatgct tctctggcgc gtaatccctt ccgcgttttt acttcattac
      541 tccgccttga gcttatcgag aacgaaattt tgcgccagaa agcggcggag attctgcgtc
      601 agcgcgatat cttcacccca cgttgtcgac aactgttaga ggaatatgag cagcggggcg
      661 gttttaacga aacacaggca caggagtttg tgcaggaagc cctggaaacg tttcgttggc
      721 accagtcagc aacggtagat gaagaaacct atcgcgcctt gcacaacgaa catcggttaa
      781 ttgctgatgt ggtctgtttt cctggatgcc atatcaacca cctgacgcca cgtacgctgg
      841 atattgaccg ggtgcagtcg atgatgcctg aatgcgggat tgaacccaaa attctgatcg
      901 aagggccgcc gcgccgcgag gtaccgattt tactacgcca gaccagcttt aaagcactgg
      961 aagagacggt gttgtttgca gggcagaaac agggtacgca taccgcgcgc tttggtgaaa
     1021 tagagcagcg tggtgtggca ttaacgccga aagggcgaca actgtatgat gatcttctgc
     1081 gtaacgctgg aaccgggcag gataatctca ctcaccaaat gcatttacag gaaaccttcc
     1141 gcacttttcc tgacagtgag tttttaatgc gtcagcaagg gttggcatgg ttccggtacc
     1201 gtctaacgcc ttcaggtgag gcgcatcgtc aggcgattca ttccggagac gatccacagc
     1261 ccttaattga acgtggttgg gtagtggcgc aacccattac ctatgaagat ttcttgcctg
     1321 ttagcgcggc ggggatcttc cagtcaaatc tgggtaatga aacgcaggca cgcagtcacg
     1381 gtaatgccag tcgcgaagca tttgagcagg cgttgggctg cccggttttg gatgagttcc
     1441 agctttatca ggaagcggaa gaacgcagta aacgtcgctg tggtttgctt taaactctga
     1501 ccatccgcct ttgcaaaaat ttgcctgatt ttacaaacga atcaggctca tcccatcgac
     1561 ataaaaaaat tgccgattta tgcatattct ctcagttcaa caattggatt attaataaat
     1621 attgtctaga gtgagcggtc ataaataagc actttcttgc cgctgaaaac gaccagcgcg
     1681 ggaccattca caacaccaga aggactcact ttcaggtatg gatcgtagac gatttattaa
     1741 aggttcaatg gctatggccg ccgtgtgcgg taccagcggc attgcttctc ttttttctca
     1801 ggcggcattc gcggcagatt ctgatattgc cgacgggcaa acccaacgtt ttgacttctc
     1861 cattctacag tcaatggcgc acgacttagc gcaaacagcg tggcgaggtg cgccgcgtcc
     1921 gttacctgac actctggcga caatgacgcc gcaggcttat aacagtattc aatacgacgc
     1981 cgaaaaatcg ctctggcata acgttgagaa ccgtcaactg gacgctcagt tcttccatat
     2041 gggaatggga ttccgtcgcc gcgttcgtat gttttctgta gatcccgcaa cacatctggc
     2101 gcgtgaaatt cactttcgcc cggagttgtt caaatacaac gatgcgggtg ttgataccaa
     2161 acaattagaa gggcaaagcg atctcggttt tgccggtttt cgcgtgttta aagcccccga
     2221 actggcgcgc cgtgatgtcg tatcattcct cggtgcgagt tatttccgcg ccgttgacga
     2281 cacatatcaa tacggtctat cggctcgcgg cctggcgatc gacacttaca ccgacagtaa
     2341 agaagagttc cccgacttta ccgccttctg gtttgatacg gtaaaaccgg gggcaaccac
     2401 ctttaccgtt tatgcgttgc tcgatagcgc cagcattact ggtgcctata agttcactat
     2461 ccattgtgag aaaagtcagg tgattatgga tgtggaaaat cacctgtatg cgcgcaaaga
     2521 cattaaacag ctgggcattg cgccgatgac cagcatgttc agctgcggta ctaatgaacg
     2581 tcggatgtgc gacaccattc atccgcaaat ccatgactct gatcgtctgt ccatgtggcg
     2641 gggcaacggc gagtggattt gccgtccgct gaacaatccg caaaaattgc agttcaatgc
     2701 ttacaccgac aacaacccga aagggtttgg tttattgcaa ctggatcgtg atttctctca
     2761 ttatcaggac attatgggct ggtataacaa acgcccaagt ctgtgggtgg aaccgcgtaa
     2821 caagtggggt aagggcacca tcggcctgat ggaaatccca actacgggtg aaacgctgga
     2881 taacattgtc tgcttctggc agccagaaaa agctgtaaaa gcgggtgatg agtttgcatt
     2941 ccagtatcgt ctgtactgga gtgcgcaacc gcctgttcat tgcccattag cgcgcgttat
     3001 ggcgacgcgt accggcatgg gcggtttccc ggaaggttgg gcgccaggtg aacactatcc
     3061 cgaaaaatgg gcgcgtcgtt ttgccgtcga tttcgttggt ggtgatctga aagctgccgc
     3121 accaaaaggc attgagccgg tgattacgct ttccagtggg gaagcgaagc aaatcgaaat
     3181 tctctatatt gaacccattg atggttatcg tattcagttt gactggtatc cgacttcgga
     3241 ctccactgat ccggtcgata tgcggatgta tctgcgttgt cagggcgacg ctatcagtga
     3301 aacatggctg tatcagtatt tcccgccagc gccggataaa cgtcagtatg ttgacgaccg
     3361 cgtgatgagt taatcgtttt ttcttcggca ccttcttcgg gaggtgccgt ctggttaaac
     3421 acgatcccgc tcgcattttt ccctaagtta aatgagtaat ctgatagtgt gtatttcaga
     3481 tacaccttgt cagccactaa cagggagtgc gtatgtttcc agaataccga gatttaatat
     3541 cccgtctgaa aaacgaaaat cctcgcttta tgtccttgtt cgataaacac aataaacttg
     3601 atcatgaaat tgccagaaag gaaggttccg acggtcgagg gtacaatgcg gaagtggtcc
     3661 gcatgaaaaa acaaaagcta cagttaaaag atgagatgct caaaatcctg cagcaggaga
     3721 gcgtcaaaga ggtgtaaact ttcctaagcc gccagccagg cggctttttt aacaactgca
     3781 tggattgact ggagataaga tgaccgaaac gataaaagta agcgaatcac ttgaattaca
     3841 tgctgttgca gaaaatcacg tcacacctct ttatcagtta atctgtaaaa ataaaacctg
     3901 gttacagcag tcgctaaact ggccgcagtt tgttcaaagt gaagaggaca cgcgaaaaac
     3961 ggtgcagggc aatgtaatgt tgcatcaacg cggatatgcc aaaatgttca tgattttcaa
     4021 agaagatgaa cttatcggcg ttatctcgtt taatcgtatt gaaccactga ataaaaccgc
     4081 tgaaatcggc tactggctgg acgaatctca tcaggggcag gggatcattt ctcaggcgct
     4141 gcaggcattg attcatcatt acgcccagtc tggtgaactt agacgcttcg tgatcaaatg
     4201 tcgggtggac aatgtgcaaa gcaaccaggt cgccttgcgc aatggtttta tccttgaagg
     4261 ttgtctgaaa caggctgagt tcctgaatga tgcctatgat gatgtgaact tatacgcgcg
     4321 tattatcgat tcacaataac cctgccagcg gcgttcgcgt aatgcgctcc tcgccgttaa
     4381 tgacttttgg accacggaca tgaaccgtgt cgccatcaaa ggcttcaacg acggcgtggt
     4441 cggttaactc cacatgtttt tcaattatca ccgcacctgt aatacggctc tcaccttgta
     4501 ttacaacgtg ctcgtcgagc agaatcggtc cgccacgtac cacggcattt ccgccaatca
     4561 atacatgatg ttttaacaca caattacctt ccacaatggc atattccgcc acctgcgaac
     4621 tgtaatgaat cgtcggaatg gcatcttctt ctatgccagc tttcacctgc gcgtgaccgt
     4681 agactttagc gcaatcgcat agccagacgt tgttctcttc attaccttca acgctggcaa
     4741 aatcaaaaac ttcggcgcga tgttcaataa aagcataccg gacgacggca tcgccataaa
     4801 tttgtgcctg gtgaacaata cgcgaggcgc taacccttgc gcgatcataa atttgtagca
     4861 ggagttgatg gtcgggcgtt aagccttgtg cggcgacgat catagaatgt tgatcaatta
     4921 aggcgtgacc aaatattcta cattgaccac aaactaatga gtcacgaatg gtgacgctgt
     4981 cacttatata tgcatcctgg ctgatttcac tattatcgat ccagacgtta tccgttgcgt
     5041 aaacctctcc ccaaagaacg ctggtgccgg tgatacgcgt attaccggaa atcaccgttc
     5101 cgccgaacgc aatagcattc tgatcgtaaa tccagcaatt tccttcctgt gctaacaccg
     5161 tttcgcgatc gatccagccg ccagcggttc ctgctatcac atcgttaaaa tcgctaatgg
     5221 cgataatctg ccgtaataac acactttttt tagtgccatc ttcctgataa ctaaaggctc
     5281 gctgttcttc acttagacga tatttgcgca tagcgttttc ccacaggtga cttactataa
     5341 ccgtagcaaa ttctgcggct ctggctatgc tcgagaaaat tccataaaat gcatttcaaa
     5401 tgtactttat aaattaaaca aaatgagtaa gaagatgcag agcgataaag tgctcaattt
     5461 gccggcaggc tactttggta ttgtgttggg gacgataggg atgggatttg cctggcgcta
     5521 tgccagccag gtttggcagg tcagccactg gttaggggat gggctagtga ttctggcgat
     5581 gatcatctgg ggattattga ctagcgcatt tattacccga ctcatacgct ttccgcatag
     5641 cgtgctggcg gaagttcgcc atccagtgct gagcagtttt gtgagtttgt ttccggcaac
     5701 gacgatgctg gtggcgattg gttttgttcc gtggtttcgt ccactggcgg tgtgcctgtt
     5761 cagttttggt gtcgtggtgc aactggctta tgccgcatgg caaactgcgg gattatggcg
     5821 cggatctcac cctgaagaag ctaccacgcc tggactgtat ctgccgacag ttgccaacaa
     5881 ctttatcagc gcaatggcct gtggtgcgtt gggctacacc gacgcaggtc tggtgttttt
     5941 aggcgcaggc gttttctcat ggctaagcct tgaacctgtg atcttgcagc gtctgcgcag
     6001 ttcgggagaa ttacccacgg cactgcggac ctcactcggc attcagctcg ctcctgcact
     6061 ggtggcctgt agtgcctggc tgagcgtcaa cggcggcgag ggtgacacgc tggcgaaaat
     6121 gcttttcggt tatggactgc tgcaactgct gtttatgcta cgtctgatgc catggtatct
     6181 ctcccagcca tttaatgctt cattctggag tttctcgttc ggcgtatctg cactggcaac
     6241 caccggtttg catctgggga gtggcagcga taatggattt ttccatacgc tggcggtgcc
     6301 gctgtttatc tttaccaatt ttattattgc aatactgctc atccgtactt ttgcgcttct
     6361 gatgcaggga aaattgttag tcagaaccga gcgcgccgtt ttaatgaaag cagaggacaa
     6421 agaatgatca ttcgtgatga aaactatttt actgataaat atgaattaac ccgcacacac
     6481 tctgaagtgc tggaagcggt gaaagtggtt aaaccgggta aaacgctgga tctgggctgt
     6541 ggcaatggtc gtaacagtct ttacctggca gccaatggtt atgatgttga cgcatgggat
     6601 aaaaatgcca tgagtgtcgc caacgtcgag cgcattaagt ccattgaaaa tctggataat
     6661 ttacacaccc gggtcgttga tctgaataac ctcacatttg atggacagta cgattttatt
     6721 ctttcgactg tggtgctgat gttccttgag gctaaaacca tcccagggct gattgccaat
     6781 atgcaacgtt gcactaaacc tggcggttac aacctgattg tggcggcgat ggataccgct
     6841 gattatccat gtaccgtcgg cttcccgttt gccttcaaag agggggaatt acgtcgatat
     6901 tacgaaggct gggagatggt gaaatacaat gaagacgtcg gcgagctgca ccgcaccgac
     6961 gccaacggta atcgtattaa actgcgtttc gccacgatgc tggcacgtaa aaaatgatcc
     7021 ggtaagaaca aagcgcgtga aaattcccca cgctgagatg gtttactgtt cttcttttcg
     7081 gtaagcatat tttttatcga agggatgtga aattaatcac agtagtcgaa gttttttagc
     7141 cgcttaactt actgaaattt aactactgat gattgactta gccccttttt cgacattgac
     7201 tatgtcgtct gaaaaggggc taaaaaattt attttcacca acactttttt tgccacaaca
     7261 cgaagcggcg ctttttgcta taacttagaa agtaatataa tcatctcagg aaactattca
     7321 tgcgtaccac atcatttgcg aaagttgcag ctttatgcgg cttgttggct ctgtctggtt
     7381 gtgcctctaa aatcacccag ccagataaat attctggttt tttaaacaat tactctgatt
     7441 taaaagaaac aacctcggca acaggtaaac ctgttttacg ttgggtagac ccgagttttg
     7501 atcaaagcaa atatgacagc atcgtctgga acccaatcac ttattatccg gtaccgaaac
     7561 cttctaccca ggtagggcag aaagttctgg ataaaatttt gaactatacc aacaccgaaa
     7621 tgaaagaagc gatagcgcag cgtaaaccac tggttaccac cgctgggccg catagtctga
     7681 ttttccgtgg ggccattacc ggtgtagata ccagcaaaga agggctgcaa ttctatgaag
     7741 tggttcctgt tgcattagtg gttgcgggga cgcaaatggc tacaggccac cgtaccatgg
     7801 atactcgcct ctattttgaa ggtgagctga ttgatgcagc gactaataaa ccggttatca
     7861 aagtcgttcg tcagggcgaa ggtaaagacc tgaataacga aagtacgcca atggctttcg
     7921 aaaatattaa acaagttatt gatgacatgg cgaccgatgc caccatgttt gacgttaaca
     7981 aaaagtagtt ccagacgcgc catcgttaga tggcgctttt tattatcggt ttctgttagc
     8041 gattaaattc aacaccacgt aacaaacgcc tccggcaatt aatccccaaa acgcagaacc
     8101 aatcccgaac agcgtcaatc cacttgccgt taccagaaat gccaccaccg ccgcgtctcg
     8161 ctcacgctca ttatgcagcg cctgatacaa actgccgcca atggtactca acagcgccag
     8221 acctgccagc atctggatcc aacttacggg cagggcagac atcatcccgg taatggcact
     8281 accaaacaga cctgcgagca aatagaaaat gcctgcaacg gcagcggcca gccaacgttg
     8341 atctttatcc ggatgcgctt ccgggctttg gcaaatagcc gcggtgattg ccgcaatacc
     8401 gacggaataa acgccgaaag gggaaaaaaa cagtgccagc aatccagtaa atacaattaa
     8461 tggcgaaaca ggagccgaat atccggctgc tttcattgct gcgatacccg gtgcgttttg
     8521 cgatgccatc gtcaccagaa aaaggggaag tgcaacgctc agactgtgag caaacgcaaa
     8581 atcaggggtg atataagtgg ggagaacggg tttaaagaca acatcagttg tgacaacgtc
     8641 accctgcgcg ataacgatga cgatcccaat aatcatcgcg gcaattaccg catagcgcgg
     8701 cgcaacggcc ctggttgcca gccataccag caacatactg ccacacaacg taaattgacc
     8761 atccaggctc gcaaacgcct gtaaaccaaa gcgtaataaa atcccggcaa gcattgccgc
     8821 cgcaagcgag tgcggaataa tgcgcatcag acgagcaaag agtcccgtta tgccgcagag
     8881 gactattaac gcgttggtga caataaaaac gccgatggct tcgttaagtg ttagtccctg
     8941 caatccggtg accaacaaag ccgcgccagg cgttgaccat gcagtgagaa caggtacgcg
     9001 ataccataat gtcagagtca gcgtactgac gcccattgcc agccccagcg ccgtcatcca
     9061 gccagagatt tgtgcagtgg tggctccggc gacaatcgct gcttgccaga ttattgccgc
     9121 tgaactggcg tagccaatta ataccgccag aaaccccgca agtagcgtgg gcggaggaat
     9181 agaaaacaga cgcatagtca cctcgtgcgt tataacgtac aggtaccgta gcatttgtcc
     9241 gttataacgc acaagtgata aacttccgtt ttgccggagg agtcgcatgg aaaatctcgc
     9301 tcgcttttta tccaccacac ttaaacaact acgtcagcag catggctgga gtctttcgcg
     9361 actggcagaa gcgacgggcg tttctaaagc gatgttaggg cagattgagc gtaatgagtc
     9421 cagcccgacg gtcgcgacat tatggaaaat tgccaccggg ttgaatgtgc cattttcgac
     9481 atttatttct ccgccgcagt ccgctacgcc ttcagtttat gatccgcaac agcaggcgat
     9541 ggtgatcact tcgctgtttc cttatgatcc acaactctgc tttgaacact tctcgattca
     9601 gatggcaccc ggtgcaataa gcgaatcgac gccccatgag aagggagtaa tcgaacatgt
     9661 tgtcgtcatt gatgggcaac ttgatctgtg cgttgatggt gaatggcaga gccttaattg
     9721 tggaaaaggc gctcgatttg ctgcagacgt tacgcatgtc tatcgtaatg gtggggagca
     9781 aatcgtacat tttcattccc tcatccatta cccgcgcagt taagcgggaa aactatttcg
     9841 caacgtcgcg cttgtggcta aaatagccgc catttttcag ctactggata agaatgtgac
     9901 cgtatcttct catcgacttg aactgttaag cccggcacgc gatgccgcca ttgcccgcga
     9961 agctattttg cacggtgccg atgctgttta tatcggcggc cctggttttg gtgcccgtca
    10021 taatgccagt aatagcctga aagatattgc cgaactggtg ccgtttgccc atcgttatgg
    10081 tgcaaaaatt ttcgtcacgc ttaacaccat tttgcatgat gatgagctgg aacctgcgca
    10141 acggctgatt accgacttct accagaccgg cgtcgatgcg ctgattgttc aggatatggg
    10201 gattctggaa cttgatattc cgccgattga actgcacgcc agtacgcagt gcgatattcg
    10261 cacagtagaa aaagcgaagt tcctctctga tgttggcttc acgcagattg tgctggcgcg
    10321 agagctgaat cttgagcaga tccgcgcgat tcaccaggcc acggacgcga ccattgaatt
    10381 ctttattcat ggcgcactgt gcgtggccta ttcgggtcag tgctacattt ctcatgcgca
    10441 aacagggcgt agcgccaacc gtggcgattg ctcgcaggcg tgccgtttgc catacacatt
    10501 gaaagacgat caggggcggg tggtttccta tgaaaaacat ctgctgtcga tgaaagataa
    10561 cgatcagact gccaacctcg gcgcgctgat tgatgctggc gtacgctcct tcaagattga
    10621 agggcgttac aaagatatga gctacgtgaa gaatatcacc gcccattatc gccagatgct
    10681 ggatgccatt attgaagaac gtggcgatct gacgcgtgct tcatcaggtc gtactgaaca
    10741 tttctttgtt ccatcgacgg aaaagacttt ccaccgtggt agcacagatt attttgtgaa
    10801 tgcccgtaaa ggcgatattg gcgcgttcga ttcgccgaaa tttatcggcc tgccggtggg
    10861 tgaagtattg aaagtggcga aagatcatct cgatgttgcc gtttccgaac cactggcaaa
    10921 tggcgacggc ctgaacgtga tgattaaacg tgaagtcgtc ggctttcgtg ccaatacggt
    10981 cgagaaaacc ggagaaaatc agtaccgcgt ctggcccaat gaaatgccag cagagttgca
    11041 caaaattcgc ccgcatcacc cactaaaccg taatcttgat cataactggc agcaggcact
    11101 gacaaaaacc tccagcgaac gtcgggtggc ggtagacatt gaactgggcg gctggcagga
    11161 acaactgatt ctgaccctca ccagtgaaga gggtgtcagc atcacgcata cgctggacgg
    11221 gcagttcgac gaagccaata acgccgaaaa agcaatgaac aatctaaagg atggtctggc
    11281 aaaactgggg caaaccatct attacgcccg cgatgtgcaa attaatttgc cgggtgcgct
    11341 gtttgtacca aacagtctgt taaatcagtt ccgccgtgaa gccgccgaca tgctggatgc
    11401 tgcacgtctt gccggttacc agcggggcag ccgtaaaccg gttgctgatc ctgcgccggt
    11461 ttatccgcaa acgcatctga gtttcctcgc gaacgtatac aaccagaaag cgcgtgaatt
    11521 ttatcatcgc tacggtgtgc agctgattga cgcggcgtat gaggcacatg aagagaaggg
    11581 cgaagtcccg gtgatgatca ctaagcattg tctgcgcttt gccttcaatc tgtgcccgaa
    11641 acaggcgaaa ggcaatatca aaagctggaa ggcgacgcca atgcaactgg ttaacggcga
    11701 tgaagtatta acgctaaagt ttgattgccg cccatgcgag atgcacgtca tcggcaaaat
    11761 taaaaatcac attctgaaaa tgccgttacc gggaagtgtt gtcgcctccg taagtccgga
    11821 tgagctgctg aaaacattgc cgaagcgaaa agggtaaaat gccagttttc tggttactca
    11881 caacttattg aatctgcatg atattgttgg ccgggtaagg cgtcacgccg catccggcaa
    11941 taaatgactc aacgttgtcc cgcttctggt ttgcgcgatt gttgccagta aaaatgctcg
    12001 cgcaaacctt ccgccgactc ttccgccaca gcacgcaatt catcgctgtc cgcttcatga
    12061 cgcagctgat gatccacatt ctttacccac acaaattcat gtcctttgtg ccctgccatg
    12121 agttgtcctg agaacagcgc acacgttaat aagacaaccg ataacgcctt cgtaaacatc
    12181 ctgccacctt tttcttacct tttgccgcta tgatgccgat cgtttcttga ggttattatt
    12241 cagttttgca aattagcgca aagaaattat ggaatcttcc ttcctgattt tgcattgcat
    12301 tctgccgttg cggcgattta gtgctatttt cgagcaaatt acacacggag gtaaacacta
    12361 atgtttactt tgttgatata ctcagcggca gggaggcgat gtgaaacaaa gcgagttcag
    12421 acgttggctc gaatctcagg gcgtcgatgt agcgaatggc agcaaccatt taaaactcag
    12481 gtttcacggg aggcgcagtg tcatgccgcg tcaccccagc gaagagatta aagaaccatt
    12541 gcgtaaagcc attctgaaac aactcggttt gagttaatcg ccaattaaaa aggttaatga
    12601 catgcgagag acagtcgaaa ttatgcgtta tcccgtcact cttacacccg cgccggaagg
    12661 cggttatatg gtttcttttg tggatatccc tgaagcgttg acccagggcg aaactgtcgc
    12721 tgaagcgatg gaagcggcaa aagatgcttt actgaccgca tttgattttt attttgaaga
    12781 taacgagctt atccctttac cttcgccatc aaatagtcac gatcacttta ttgaagtgcc
    12841 tttgagcgtc gcctctaaag tattgctgtt aaatgctttt ttacagtcag aaatcactca
    12901 gcaagagtta gccaggcgaa ttggcaaacc taaacaggaa attactcgct tatttaactt
    12961 gcaccatgcg acaaaaatcg acgccgtcca gcttgcggct aaggcgcttg gcaaagagtt
    13021 atcgctggtg atggtttaat tacagttaac gaaaagttgt catttttaac aactgatata
    13081 gactgccgaa tcatctgcac ataattacga ttcgataatg aaaaaatacc agcagcttgc
    13141 agaacaatta cgcgagcaga ttgcgtcggg tatctggcaa cctggcgatc gtttgccttc
    13201 gttgcgtgac caggtggcgc tttctggcat gagctttatg accgtgagcc atgcctatca
    13261 gttgctcgaa agtcagggat atattatcgc gcgaccgcag tcgggttatt acgttgcgcc
    13321 acaggcaata aaaatgccga aagcgccagt cattcctgtc actcgagatg aagcagtcga
    13381 tatcaacacc tatatttttg atatgttgca ggccagtcgc gatccgtcgg tagttccgtt
    13441 tgcctcggcc tttcccgacc cgcgactttt ccccctccaa caactcaacc gctcgctggc
    13501 gcaggtaagc aaaaccgcca cggcgatgag cgtgattgaa aacttaccgc caggaaacgc
    13561 agaactgcgt caggctattg ctcgtcgcta tgccttacag ggcatcacta tttctcctga
    13621 tgaaattgtc attaccgccg gggcgttaga ggcattaaac ctcagtttac aagcggtcac
    13681 tgaaccgggc gattgggtga tagtagagaa tccttgtttc tacggcgcgt tgcaggcgct
    13741 ggagcggtta cggctgaagg cgttatcggt ggcgacggac gttaaagaag ggattgatct
    13801 caaggcgctg gaactggcgt tgcaggagta tccggtgaaa gcgtgctggc tgatgactaa
    13861 tagccagaat ccactcggat ttaccttaac gccgcagaaa aaagcgcggc tggtagcgtt
    13921 gctcaatcag tacaacgtaa cgctgattga agatgacgtg tacagcgaac tttattttgg
    13981 acgggaaaaa ccgctgcctg cgaaagcgtg ggatcgccac gatggcgttt tgcattgctc
    14041 ttcgttttcg aaatgtctgg tgcctggttt tcgtattggt tgggtcgccg ccggaaaaca
    14101 tgcacgtaaa attcaacgct tgcagttgat gagtacgctt tccaccagct caccgatgca
    14161 gcttgcactg gtggattacc tttccacgcg ccgatacgac gcccatcttc gtcgcttgcg
    14221 tcgccagctt gcggagcgta aacaacgtgc ctggcaggca ctgctgcgtt atctgcctgc
    14281 ggaagtgaaa attcatcata atgacagcgg ttattttctc tggctggagc tccccgcgcc
    14341 gttagatgcg ggcgaattaa gcctggcagc actgacgcat catatcagta ttgcgccggg
    14401 taaaatgttt tctaccggtg aaaactggtc acgttttttc cgttttaata ccgcgtggca
    14461 gtggggcgag cgcgaagaac aggcggttaa acaattaggc aaacttattc aagaacgact
    14521 gtaatagcgt ttaatttaat tcctctcagg ttaagtaata tgaatttcga atagcagtca
    14581 tatttcctaa ctccttgtct atactccaga agataacctt acagacggca taatgcgcgg
    14641 tagctcacaa cctgattaaa ttttctcagg ggcgaaggtg tgcctgctag ccgccgtcta
    14701 tggttaaaca aggagatatt tttacggcac ggcggctgaa caattaatta cgacaggagt
    14761 aagactttat gagcaagaca tttgcccgca gcagcctgtg tgcgctcacc atgacaataa
    14821 tgaccgctca cgccgccgaa ccacctacca atttagataa accggaaggg cgactggata
    14881 ttatcgcctg gccgggatac atcgaacgcg gacaaactga taaacaatac gactgggtaa
    14941 cgcaattcga aaaagagact ggctgcgcgg tgaatgtgaa aaccgccgcg acttccgatg
    15001 aaatggtcag tctgatgacc aaagggggtt acgatctggt tacggcatcc ggcgatgcct
    15061 cgctgcgcct gattatgggc aaacgcgtgc agccgattaa taccgcattg attcccaact
    15121 ggaaaacgct cgatccgcgc gtggttaaag gcgactggtt taacgttagt ggcaaagttt
    15181 atggcacacc ttaccaatgg ggtccgaacc tgttgatgta caacactaaa accttcccga
    15241 cgccgccgga tagctggcaa gtggtttttg ttgagcagaa tctgccggac ggcaagagca
    15301 ataaaggccg cgttcaggct tatgatggcc ctatctacat tgcggacgct gcgttgttcg
    15361 ttaaagccac tcagccgcag ttgggcatca gcgatccgta tcaactcacc gaagaacagt
    15421 accaggcggt gctgaaagtg ctgcgcgatc aacatagttt gatccatcgc tactggcatg
    15481 acactaccgt gcaaatgagc gatttcaaaa acgagggtgt ggttgcttcc agtgcatggc
    15541 cctatcaggc caacgccctg aaagccgaag gccagcctgt cgcgacccta ttcccgaaag
    15601 agggcgttac cggttgggct gacaccacca tgctacatag tgaagcgaaa catccggttt
    15661 gcgcctacaa atggatgaac tggtcattaa cgccaaaagt gcagggcgat gtggcggcct
    15721 ggtttggctc gttaccggta gtgccggaag ggtgtaaagc cagtccgtta ttaggcgaga
    15781 aagggtgtga aacaaacggt tttaactatt tcgataaaat cgccttctgg aaaacaccta
    15841 tagcagaagg gggcaagttt gttccctaca gtcgctggac gcaggattac attgccatta
    15901 tgggcggtcg ctaacttcgc tggggtgttt tatgacgtac gcagtggagt ttggcaacgt
    15961 ctcgcggttg tacggtgacg tgcgcgcagt agatggcgtc agtattgcga taaaagatgg
    16021 tgagttcttc tctatgctgg ggccgtccgg ctccggcaaa accacctgcc tgcgcctgat
    16081 tgctggcttc gaacagcttt ccggcggggc tatctctatc tttggtaaac ccgccagcaa
    16141 tctgccaccg tgggagcggg acgtgaatac tgtcttccag gactacgcac tatttccgca
    16201 tatgtcgatt cttgacaacg tcgcctatgg tctgatggtc aaaggcgtga ataaaaaaca
    16261 gcggcatgcg atggcgcaag aggcgctgga gaaagtggca ttggggtttg tacagcaacg
    16321 caaaccgtca caactttctg gtggtcagcg ccagcgagtc gccatcgcca gagcactggt
    16381 gaacgaaccg cgtgtgttat tgctggatga accgctcggc gcactggatc tcaaattgcg
    16441 tgagcagatg cagctggaac tgaaaaaact gcaacagtct ctcggtatca cttttatctt
    16501 cgttacccac gatcagggcg aagcgttatc gatgtccgat cgtgtggcgg ttttcaataa
    16561 tggacgcatt gagcaggtcg attccccgcg cgatctctat atgcgcccgc gcacgccgtt
    16621 tgttgccggg ttcgttggta catcgaatgt ttttgatgga ctgatggcag agaaactttg
    16681 tggcatgacg ggaagcttcg ccctgcgacc ggaacatatc cgcctcaaca cccctggtga
    16741 actgcagacc aatggcacga tccaggcggt gcaatatcag ggcgcggcaa cacgttttga
    16801 actgaaattg aacggcggtg aaaaactgct tgtgagtcag gccaatatga caggcgaaga
    16861 actgcctgcc acgctcacgc ccggacaaca ggtgatggtt tcctggtcgc gtgatgtgat
    16921 ggtgccgctg gttgaggaga ggtgaatggc gatgaatgta ttgcaatcac cttcacgtcc
    16981 aggtctgggt aaggtgtccg gtttcttctg gcgtaatccg gggctggggc tgtttttact
    17041 gctgcttggc ccgctaatgt ggtttggcat tgtctatttc ggctcgctgc tgacactgtt
    17101 atggcaggga ttttatacct ttgacgattt caccatgtcg gtaacgccgg aactgacgct
    17161 ggcgaatatc cgtgcgctgt ttaatccggc gaattacgac atcattctcc gcacgctgac
    17221 tatggctgtg gcagtcacta tcgccagcgc cattctggct tttccaatgg cgtggtatat
    17281 ggcgcgctat accagcggga aaatgaaagc gtttttttat attgcggtaa tgttgccgat
    17341 gtgggcgagc tacattgtta aagcctatgc ctggacgtta ttgctggcaa aagatggcgt
    17401 ggctcagtgg tttttacaac atcttgggct ggaaccactg ctgactgcgt tccttacatt
    17461 acctgcggtg ggcggcaata cgctgtcaac ttccgggctg gggcgctttc tggtgtttct
    17521 ctatatctgg ctgccgttca tgatcctgcc cgtgcaggcg gcgcttgagc gtttgccgcc
    17581 gtcattgttg caggcgtcgg ctgatcttgg cgcgcgtcca cggcaaacct ttcgctatgt
    17641 ggtgctgccg ctggcaatcc ctggtattgc cgctggctct atctttacct tttcactcac
    17701 actgggcgat tttattgttc cgcagctggt tggccctcca ggatatttta tcggcaatat
    17761 ggtttattcc cagcagggag cgattggcaa tatgccgatg gcggcggcat tcaccctggt
    17821 gccgattgtt ctcatcgcac tgtacctggc gttcgtgaaa cgtctgggag cgttcgatgc
    17881 actctgaacg cgcaccgttt ttcctcaaac tggcggcctg gggcggcgtg gttttcctac
    17941 attttcccat cctgattatc gccgcctatg cgtttaacac tgaagatgcg gcgtttagtt
    18001 ttccaccgca gggcctgacg ctgcgctggt ttagcgtggc agcacagcgt agtgatattc
    18061 ttgatgccgt gacactgtca cttaaagtgg cggcgctggc gacattaatg gcgctggtgt
    18121 tagggacgct ggccgctgcc gcgctgtggc gacgagactt tttcggcaaa aacgccattt
    18181 cgctgttact gctgctgccc attgcgctgc cgggcattgt cactggtctg gcgttgttaa
    18241 ccgcctttaa aaccatcaat ctggagccgg gatttttcac catcgtggtc ggtcatgcga
    18301 ctttttgtgt agttgtggtg tttaacaatg tcatcgcccg ttttcgccgc acctcgtgga
    18361 gtctggttga ggcgtcaatg gatcttgggg ccaatggctg gcaaaccttc cgctacgtag
    18421 tgttgccgaa tctcagttcg gcgttactgg caggaggaat gctggcgttt gccttgtcgt
    18481 tcgatgaaat catcgtgacg acctttacgg caggtcatga acgaacgtta ccgttgtggt
    18541 tgctcaatca gcttgggcga ccgcgtgatg taccggtaac caacgtggtg gcactgctgg
    18601 ttatgttggt aacaaccttg ccgatcctgg gggcctggtg gctaacccgc gaaggcgaca
    18661 atggtcaata accactgata caggaatatg ctatgcaaca taagttactg attaacggag
    18721 aactggttag cggcgaaggg gaaaaacagc ctgtctataa tccggcaacg ggggacgttt
    18781 tactggagat tgccgaggca tccgcagagc aggtcgatgc tgctgtgcgc gcggcagatg
    18841 cagcatttgc cgaatggggg caaaccacgc cgaaagtgcg tgcggaatgt ctgctgaaac
    18901 tggctgatgt tatcgaagaa aatggtcagg tttttgccga actggagtcc cgtaattgtg
    18961 gcaaaccgct gcatagtgcg ttcaatgatg aaatcccggc gattgtcgat gtttttcgct
    19021 ttttcgctgg tgcggcgcgc tgtctgaatg gtctggcggc aggtgaatat cttgaaggtc
    19081 atacttcgat gatccgtcgc gatccgttgg gggtcgtggc ttcgatcgca ccgtggaatt
    19141 atccgctgat gatggccgcg tggaaactgg ctccggcgct ggcggcaggg aactgtgtag
    19201 tgcttaaacc atcagaaatt accccgctga ccgcgttgaa gttggcagag ctggcgaaag
    19261 atatcttccc ggcaggcgtg attaacgtac tgtttggcag aggcaaaacg gtgggagatc
    19321 cgctgaccgg acatcccaaa gtacggatgg tgtcgctgac gggctctatc gccactggcg
    19381 agcacatcat cagccatacc gcgccgtcca taaagcgtac tcatatggaa cttggaggca
    19441 aagcgccagt gatagttttt gatgatgcgg atattgaagc ggtggtcgaa ggtgtacgta
    19501 cttttggcta ttacaatgcc ggacaggatt gtactgcggc ttgtcggatc tacgcgcaaa
    19561 aaggtattta cgatacgctg gtggaaaaac tcggtgctgc agtggcaacg ttaaaatcgg
    19621 gtgcaccgga tgacgagtct acggagcttg gacctttaag ctcgctggcg catctcgaac
    19681 gcgtcagcaa ggcggtagaa gaggcgaaag cgacagggca catcaaagtg atcaccggtg
    19741 gtgaaaagcg caagggtaat ggctattact atgcaccgac gttgctggct ggcgcattac
    19801 aggacgatgc catcgtgcaa aaagaggtat ttggtccggt agtgagtgtt acgctcttcg
    19861 acaacgaaga acaggtggtg aactgggcga atgacagcca gtacggactt gcatcctcgg
    19921 tatggacgaa agatgtgggc agggcgcatc gcgtcagcgc gcggctgcaa tatggttgta
    19981 cctgggtcaa tacccatttc atgctggtaa gtgaaatgcc gcacggtggg cagaaacttt
    20041 ctggttacgg caaggatatg tcactttatg ggctggagga ttacaccgtc gtccgccacg
    20101 tcatggttaa acattaagga taatattgca gatcgtaaga gtatgagaag atcttgcgta
    20161 ctgtcagagc actgatttct ctgacagtac gtgctgcgtg caaaatcact acatcaaata
    20221 aaccagccaa atctcataga gataagagta aacaaaccaa cagcagcaaa gacatttatc
    20281 agtactacag ttctactgga aacattcatt tttgccacct tttatcattg cccctatgtg
    20341 tatagtacgg gttaagaaaa tccgcaataa tactcattct gaagatggat aagggcaagt
    20401 tgctgtttga tgattttttc tcaaactggt gataaaactt gatgcacgtc aaaaaatgac
    20461 gcatatttgc gcgcgtttta ttcatctggc tggacgcccg tacatgtctc tctatcaaca
    20521 catgcttgtt ttttatgcgg ttatggcagc aatcgcattt cttatcacct ggtttctttc
    20581 tcacgataag aaacgcatcc gtttcttaag cgcctttctg gtgggggcaa catggccgat
    20641 gagtttcccg gtggcgctgt tgttttcact gttttaaatc ttactcactg gcttgacggc
    20701 accacgcaga cttatatcat ttggatgaat cgataaattt cacaagtggc taaggagaaa
    20761 gtatgtcgca tctggatgaa gtcatcgcgc gcgtggatgc cgcgattgaa gagagcgtca
    20821 ttgcccatat gaacgaatta ttaatcgccc tgagcgatga cgcggagtta agtcgggaag
    20881 atcgctacac ccagcagcag cgtctgcgca cagcgatcgc ccatcacggt cgcaagcata
    20941 aagaagatat ggaagcgcgc cacgaacagt taaccaaagg tggcaccatc ctctgattaa
    21001 aatgtgcgcc tggccaccag ccaggcacca accacaagta aaattgcgcc acaaattggg
    21061 ccgatcaacg cccgtagtgg tacgccatgg ctgcggaaga tatccagcac cagaccgcca
    21121 atcagctggc tggcgaccag tgccgcaata gttgtcgccg ctcccacatt ctgatatccg
    21181 ctgatactgg cgaagacaaa aaacgatccc agcaagccag gtataagtgt ccaccagcgc
    21241 acgctggaca ccagttcgcc aaaccccgcc atgccttgtt taaaccataa aatagaaacg
    21301 aacagcacaa ttcctaccaa agagttcagc aacatggcga tgagaatggt agaggaggtc
    21361 tgggtgatgc gcaccatcag cgtgttctgg actaccagac caattccggc ggcaatcaaa
    21421 aaagcaaggg taagcgactg attcatccaa tcgcgtccgg ttcagtgcgc tcgtcgagtt
    21481 gcaactgcat aaatgtcaga tccagccagc gcccaaattt agtgcctacc tgcggcattt
    21541 gtgcggtgac gacaaatccc aacgactggt gcagatgcaa agacgcctga ttttgcgatt
    21601 cgatcccggc gaccatgaca tgcttcccgc aatcccgcgc ttcatcaatc aatcggctta
    21661 acaatttacg cccaagacct ttgccctgat gatcgggatg gacataaacc gaatgttcca
    21721 cggtatggcg aaaaccatcg aaactacgcc agtcgccaaa cgaggcatat cctgtaacga
    21781 cgccatcttc ttcactcacc agcaccggat aacctgctaa agtccgcgct tcgaaccagg
    21841 caatgcggtt atctgcatcc accgtttggt cattccagat agccgccgta tacaacacgg
    21901 cgtggttata aatttccgca atggcagcac agtcggcttt gcgggcaaaa cggatggaca
    21961 tgtctggcct cgataaaata atgatgatga tgttatcaat actatcgaaa acagcccgaa
    22021 gcaacggatt cctcgatgaa attattgcgt gttgcagagg ggatgtgacg gctgcaaaca
    22081 aaaccaatcc cgtgagcctg ctactattga tggattcatg ccaaaagcac aggagagcat
    22141 tatggggcaa caaaagcagc gtaaccgccg ttgggttctg gcctcgcgtc cgcatggcgc
    22201 accggttccg gagaatttcc gtcttgaaga agatgatgtc gccacaccgg gtgaaggaca
    22261 ggtgttactg cgcacagttt atttgtccct ggacccgtat atgcgtggac gtatgagcga
    22321 tgagccatct tattcaccgc ctgttgatat tggcggcgtg atggtcggcg gcacggtgag
    22381 ccgtgtcgtg gagtcgaatc atcctgatta tcagcctggc gactgggtgc tgggctacag
    22441 tggatggcag gactatgaca tatccagtgg tgatgatttg gtgaaacttg gcgatcatcc
    22501 gcaaaatcca tcgtggtcgc tgggtgtgct ggggatgcca ggctttactg cttatatggg
    22561 cctactggat atcggccagc ctaaagaggg cgaaacgctg gtggtagctg cggcgacagg
    22621 accagtaggg gcgacggtgg gtcaaatcgg caaacttaaa ggttgtagag tggttggggt
    22681 ggccggtggc gcggaaaaat gccgccatgc tactgaggtg cagggcttcg atgtttgtct
    22741 tgaccatcac gcggatgatt ttgccgaaca actggcgaaa gcgtgcccaa aaggcgttga
    22801 tatctattat gaaaacgtgg gcggtaaagt atttgatgcg gtgctaccgt tacttaatac
    22861 atctgcgcgc attcccgtct gcggcttagt gagcagctat aacgctacag agctaccacc
    22921 cggcccggat cgtttacctc tgctgatggc tacggtgctg aaaaaacgta ttcgcttgca
    22981 aggatttatt atcgctcagg attatggtca ccgcatccat gagtttcaga aggagatggg
    23041 gcaatgggtg aaagaggata aaatccacta ccgcgaagaa attactgacg gtttagagaa
    23101 tgcgccacag acatttatcg gcctgctgaa gggtaaaaac ttcggcaaag tggtgatccg
    23161 cgtggcgggt gatgattaaa gatacgacgg cggcgtaagc cgccataaaa atccaggcaa
    23221 aaagcttatt ttcagcttta attaactaac tacgtcattg ttctgctgct aataacaata
    23281 aaggtgagtg gtttatgatt attcacttta ataccccagg tgaactcctt ctgccatgca
    23341 ggcagggttg gacagaaaac acgcgaagga attaaaatgc ctggaacgga aaaaatgaaa
    23401 catgtcagtt tgactctgca ggttgagaat gacctgaaac atcagcttag tattggcgca
    23461 ctaaaacctg gcgcacgcct gattactaaa aatctggcgg agcaattagg tatgagtatt
    23521 acacctgtgc gtgaagcatt attacgtctg gtttcggtga atgcgctttc tgtcgcacca
    23581 gcgcaagcat ttacagttcc ggaagtgggg aaacgtcaat tagacgaaat caatcggatc
    23641 cgctacgagc tggaattaat ggcagttact ctggctgttg aaaacctcac ctcgcaagac
    23701 ctggcggaac tcgaggaatt gctcgagaaa ttacagcagg cgcaagaaaa gggcgacatg
    23761 gaacaaatca ttaatgcgaa caggctattt cgcttagcaa tttatcatcg ttcaaatatg
    23821 cccatcctgt gtgagatgat tgagcaactg tgggtcagga tgggacctgg tttacattat
    23881 ctttatgaag cgattaatcc agcggaatta cgggaacgta tagaaaacta tcgtctatta
    23941 ctcgccgcgt taaaagcaaa agacaaagag ggatgcagac attgtcttgc tgaaattatg
    24001 caacaaaata ttgctatttt ataccagcaa tacaatcg
//
LOCUS       DACBVI010000061        24005 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.061, whole genome shotgun sequence.
ACCESSION   DACBVI010000061 DACBVI010000000
VERSION     DACBVI010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.061"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..739
                     /locus_tag="IU325_11885"
     CDS             <1..739
                     /locus_tag="IU325_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809723.1"
                     /translation="CCLLTEYIKKYKMNNGLSLLDLDLFRGISSNYRVVVQLLISSCL
                     SGDSDTIYDIQDELPFSWGWIPVSIWKDVFQKCWAYLEKQINDKTLALHILQPFIAFM
                     NHRAHIDRRLAPIANMLLTYSELLPAGCNVLPTVSREQFNEAKQMLLRNPDSFGRISI
                     FPKELWSSAITPELKSVFNKLWIEDKYHSRLEKRFNLMLVAALLTQKDNNLIYQLSAL
                     FEFHYQQAPQQLGVIYQYYFEQAGVCH"
     gene            739..7053
                     /locus_tag="IU325_11890"
     CDS             739..7053
                     /locus_tag="IU325_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP5809724.1"
                     /translation="MDVQQLHYAPLLNQLHQRAVRSVVSQLALRSKSLTHYLESQYFQ
                     PPGQEGSLLADPVFESTFGWMPADETMEDLRDKLISSPLVDALSKAPVPFRHQLTAWR
                     TILEDKKSLLVSSGTGSGKTECFMVPVLEDLIRQQKSTRQSLTGTQAIFLYPLNALIN
                     SQQERLREWTRGFKGKIRFALYNGETRHTKYEVQEDQLKVPEQALSREAIYEAPPSIM
                     VTNTTMLEYMLIRRKDAPIIEKSQGILKYIVLDEAHSYIGSQAAELALLLRRVMQAFN
                     VGPGTDKPVQIIATSATIGEDSPEGNKELAKFVADLAGVTERDVKVVRGYRQIPRVSE
                     SLIRHEYPTSLTSLKSLSPQDLYQQLCHYRVAQQLRQALTHPGRQAVRLSELLNVARR
                     TWPDINHRELLQLLDLMARSREGELAFTPLRMHGFIRTLAGLWACSNKQCLHKAHELN
                     QSDWPFGQVWFEQRQYCDCGAPVFEVLRCSGCGSAYLSAKEEVRGDGTNWLVAQPAAA
                     EVDEFALDVDVYSEDEDNDELDNNSQFDRLIAIDGEKYIISLEETTAGKIDSEAQKHY
                     EINLLRPESRGEGRNNSFACVCCGDTQRKNNPLFRPLRLGAPFFLNEIIPTLLEFSPL
                     PQTREEQLGPFNGRRLLTFTDSRQGTARISARLQQDADKATLRALCYQELANINEPSA
                     KLSPAHCALLESFITQFSILPLKNSIDALKKAMVANEELSPADIQALKIIEPMVSSLS
                     PDHAEAMQILQANSGNQQKKTMSWQTLSEQLVGSVDLADRMRGSFKELSGLDLTKQQF
                     ADFCLYSEFGRRPKNAWTLESMGLVAIHYPFIDKVTTCPQDWKTLLPDPDKQLAEWRN
                     LLKITLDFFIRENSAVFYENEQYPRWMGARFPVKMLQGPDKKNKGQKRDQLWPQIRDR
                     HYNRVIKLLIAVFPAIQPEQAHWKSLVNHLMIEVWDAIRPCLRQFESGYQLDIKQQAE
                     FYSPKQVWRCPYTRRALDVTLLGYSPYLPGSKEIAPEKAVLIEMPELPVRHWRLSGGG
                     EIAREERLEWLESNALIQHAREEGLWSTRSDRLALKDSWYRLEEHSAQRTPEQNQFNE
                     KQFKSGKVNVLNCSTTMEMGVDIGGMSLVAMNNVPPAPANYLQRAGRAGRRGESASAA
                     ITLCKNTAHGMEVFKDPLWAFNTTASAPRVRFGSSSIVQRHVNALVLGLFLRAEVPDA
                     TKLSCKWFFEGDESQCLRFLHWLNNQADQLADKLKRLTQGTVLMSLTATQLLTRTQAM
                     MQQVDIRWRSQLAILLENIEALKADNSAWEETPAGKAIAYQLRDYRGAYLFSKLISEA
                     FLPGHGFPVGVVNFNYLTADELEKRRAIKATQADPNEGGESFSRRIEKLPSRDLPTAL
                     REYAPGADVVLGGKVYRSSGIMLGKVLASGQELSGDHHIPWFWHCRKCGAGATSTTRP
                     VECSHCKADIQQLDVKRYLQPVGFATDIRYQAHNDVSMPAQLPWKDPRVLVPSSVWVS
                     LPDAGLGRYRFSHSGELFHFSEGEFGHGYAICLSCGRAESQNQPQRTPENLKNPEREN
                     THYLLRGGSNDRLGSNKLCHGHVHKDLWLGYSSRTDMVELQLNDDNGLLIRDEVAARS
                     LAVALREGLAHKLGIENTELGVTTQQARDINGYTGYSIFIYDNNAGGAGYAVQLIDHW
                     ADVFNYARKLLDCSCDKFCHHCLLSYDSQHYVNRLDRHHALTLLTNVRLQRLNLAPEY
                     QYFGDGSRVETNPLSLRIAQCLNSEIYDSCSLVLAGPQEQWDFAQWPLFKELLQFASS
                     GGNVELLVATPLANLTDSSRHQLSALAAMPGGRLQVKSIATAQLMQGKGRWLAQVTRE
                     GQSQQWAADDSATVAPGELWGQSASSPVVTLKGTSGKTFSGQTLSAEDLLPALPTGAV
                     RINLCEQLDGPLEGFGSRFWSLVTQQHAGWKQAFTRHKEITHVEYSDRYLNSPFTARL
                     LGEILTELVEQGMAERASLTVCVKKLDYNSRQHDALYNAWLNEEDRQQVVTTLLEEGY
                     LGPAWPGAISWLTGDNQSTEHGRELTVTFSDGSQHYVLLDMGLSYWRCIEDTFFDFAL
                     RVPQQVERLANTRARAVASGNDLRSYVIAG"
     gene            7277..10135
                     /locus_tag="IU325_11895"
     CDS             7277..10135
                     /locus_tag="IU325_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP5809725.1"
                     /translation="MNKQNYAPGMRVVIRDAEWRIRRADDSGDGGYLLTCDGISELVR
                     GKEGLFLTKLEQKVEILDPAKTHLVEDESANYQAAQLYIESQLRQRVPTDSKVHFGHL
                     AAMDSMPFQLDPTRMALAQPRQRILIADAVGLGKTLEAGILVSELIRRGRGKRILVLA
                     VKSMLTQFQKEFWSRFAIPLTRLDSAGLQKVRNRIPTNHNPFHYFDKTIISIDTLKQD
                     IEYRHHLENAWWDIIVIDEAHNVAERGTSSLRSKLAKLLAGRSDTLIMLSATPHDGKA
                     ESFASLMNMLDPTAIANPKEYEYADFADKNLVVRRFKKDVKDQMSGEFPERNIVKLTR
                     LASGAEEEAYRRLVESQFRDDDDEQAQSNKGRLFKITLEKALFSSPMACASVVANRLK
                     RLESRKDHNSQSQINELESLLLALNNIDASQFSKYQLLLDTIRKDLAWKANNTEDRLV
                     IFTESIKTLEFLEQQLRADLKLKDDQIATLRGDQGDTVLMETVEAFGKTQSPLRLLVC
                     SDVASEGINLHHLSHKMIHFDIPWSLMVFQQRNGRIDRYGQKHQPQIRYLLTEASEPQ
                     INGDMRVLEVLINKDEQAQKNIGDSSEFTGKFTQEEEEEQVAEFMMQDDGASLFDQLL
                     NSNVSESAEHDLFGEICSAVSSDASMVIETDTSLFASEQAYCERALGYLKASGQTIQY
                     ETLPDNTLSLVAPEELRRRFNQLPPEIAPENWQLFLSQDKTVITDAIARARGEQHAWP
                     DVQYLWQINPVVQWLDDKISSAFGRHQAPVIRLPYLLEPDEDHFILSGLFPNRKSHPM
                     VNPWIVVSFNRESLIGSQPFAEFLQRHLQLSNKLTNSGGKDRNHQRQQDLLEAAIAHA
                     REVFIHDRNAFETHINQQLNEHLQKLDVLRGRQLSQLELDFADNKQQLSVKQSRKEQR
                     QREIEHNFDSYIEWIEDTMTTEKEPYIQVIAVITGAEG"
     gene            10138..15063
                     /locus_tag="IU325_11900"
     CDS             10138..15063
                     /locus_tag="IU325_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809726.1"
                     /translation="MALVGINNENEFYSNHYLGEVFTSDIRDVLEPWIAQENAAREAE
                     RAAREQGKDVEPGYRAPWNQFNSLATEFFRKLAEHEKQRQIPQRLADQRNRWQPLLKA
                     LGYEITPQIQMLDDDTPLPVLARYNSTDGSPWLWIVEAHDQEEGTLDPLALSLLTAQF
                     PADTDKHKRDSLRKKANGEYRSWQDLLSTAVFTQNEPPRFVLLLGNRQLLLLDRTKWA
                     QNRLLRFDFEEILSRRETDTLKATAVLLHKDSLLPGSGAPYLDSLDDNSHKHAFGVSE
                     DLKYALRESIELLGNEAMHYLIDRGLANYTGNRAVDPDELSRECLRYMYRLLFLFYIE
                     ARPELGYAPMTAKTYLQGYSLETLRDLEMIPLTSEEDRNGRYFHDSLNMLFKLVREGY
                     NGGVKMQSDLESGDRITIHSHQFSVPRLESHLFDANNTRILNRVVFRNETLQQIIQAM
                     SLSRPGKGRFNRRGRISYRQLGINQLGAVYEALLSYRGFFASEDLYEVKKAGEEFNEL
                     ETGYFVSKDEIGKYHEDEKVYEKDGSLRIHRKGSFIYRMAGRDREKSASYYTPEVLTR
                     SLVKYALKELFKEQIDPISDPHAKADAILNLTVCEPAMGSAAFLNEAINQLAEAYLFH
                     KQQAEGRRIPQDRYTQELQRVKMYIADNNVFGVDLNPVAVELAEVSLWLNAISGDAFV
                     PWFGYQLHCGNSLVGARRQVFNKSELTYKKAKDPSWLNSEPVELAMNTPREETQIFHF
                     LLPDGGMANYSDKTVKQRYPDDFKALDSWRKEFIKSFAGHEIADVQRISEKVEALWNT
                     YRQQLKAERLKTADSYPVWPAENTEQTRSSLSSKDETFSGRLEDNSAYQKLRWVMDYW
                     CALWFWPIDKADELPDRGTWLFEIETLLDGIVITEKVTEVAEHTTGDLFAEDGLLREE
                     SSLFSGAGRLKTEVLFRHLPRLAIVDALRKQHRFFHWDLEFCDLFAERGGFDLMLGNP
                     PWLKVEWQEAGVLGDYEPEFVLRKLSASKLATLRIDTFNQIPALEAAWRSEYEGCEGM
                     QNFLNAQQNYPVLRGVQTNLYKCFLPQAWRLGAQKGVSGFLHPEGIYDDPKGGLLRAA
                     VYPRLRAHFQFANAYLLFNEVHDQTRFGINVYGPYQQSISFVSMANVFLPQTIDASFA
                     HKNYSEQVPGIKFDSALEAGSSVLWNNNGHNSRLINVGPKELTLFARLYDSEGTPAWQ
                     ARLPALHAKQLVAVLEKFANQPKRLGDLQGQYLSLEMWHETNQQKDGTIERKTQFPED
                     ASQWVLSGPHFFVGTPFYKTPRENCTLNSDYDCLDLLTLPDDYLPRTNYIPACDAQEY
                     AKRTPRVSWKEQDEDEPRKVTDYYRFVNRRMFGGSSERSFISAILPKGFANINTCVAT
                     AFESTNVLVNFTGLSHSIVYDFILKSTGKSDLYGAQLGGFVYTESFGVKIRTLLLNAL
                     SSEYAELWQSCYTPDFNTQRWSRDLPQLPQDFFAKLTPEWQRNCALRSDYSRRQALVE
                     IDVLVAQSLGLTLEELLTIYRVQFPVMRQYEADTWYDQNGRIIFTPSKGLVGVGLPRT
                     ARKADLKNGFVFNVDSPEWTGGDCTDQAIGWDDVKHLKTGTVSVTFDDYTRSDEGERR
                     TVTWQAPFIKPDREDDYKVAWAFFAQDKESA"
     gene            15063..21404
                     /locus_tag="IU325_11905"
     CDS             15063..21404
                     /locus_tag="IU325_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP5809727.1"
                     /translation="MLPSVVSRQVADSVAAFLRAAFPLNSPLFNGEENNNVSMLEQFL
                     AQPETLLKGPYLSAQLPFRKSDLPLNFFPNLTLPFPPHAHQARAFQRLGSDAPQPTLV
                     ATGTGSGKTECFMFPLLNHCAGASQAGVKAIIIYPMNALATDQASRFAKTIASDPQLH
                     GKVTVGLFVGDSEIEPSKKMSAEKVITCKHTLRESPPDILLTNYKMLDYLLMRPGDQP
                     LWRYNQPGSLRYLVVDELHTFDGAQGSDLACLVRRLKHHIGVDNQQFACVGTSATVGD
                     ELGQLLDYAKTIFDQPFTDDAVIREDRYTAAEYLQDYTIAYSQYPGPEVASALDPQGY
                     ATPVAYLNGQIPLWFPDSDLQLPDDLESDDGREKRIALGSLLRRHSVMHVLLHDLQGG
                     ILSERECLENLQVMLAESADHARRVLQSILALIAQARLEVPETEHDRQKRLQAKKARP
                     VLPFVQLRSQLWLREMRRLVASVAKTPRLVFADDVAVEDREHYLPVIHCRDCHATGWA
                     SLRHGQSVQLETDLDGIYRQFFEKGRSIVLAFPDNNEKAVSGAHQKLCPTCLKLNRQS
                     SVQCGHCGHTELLQVLIPDMLKERKDELEFANECPYCNSKQGISILGSRAASLSAVMI
                     HQLYGSQFNEHKKLITFSDSVQDAAHRASFFTARTWPLMIRGQIASVLGEKETLPLDA
                     FAARVCQQTRERSPDDAHFAANFIAPNMQWLNDYQTLKNEGKLAENSDLPELVSLRLD
                     WEVYSEFTLRARIGRTLEATGYAVAGVDKDRFNPAITALHQQLCEELGDEMSGVSVEQ
                     VAHLALGVIWYQRQAGAVMHPAFESYRRDGTTFMMTQKERTSRYMPSIGPKTRKPAYA
                     SFEKINGFHRLIGAKSNPSWYQHWINRTLSNGNNLFISSVAETVLRRLFTALKIAGVV
                     KDFDTKGREAWGLVPSALVVSRDVVQLNCSCCREVVRAPADQGWQWRNAPCLSLRCEG
                     RYQEVDNTATWRWENMDIARVQGAEHTGLLSREDREATEKSFYRGNQPWNINLLSATP
                     TLEMGIDVGDLSTVLLCSVPPAQANYLQRIGRAGRKDGNALNITVAEGNPHDQFFFEE
                     PLEMMQGQVQAPGVFLNATAILERQLAAFCMDNWVKTGVPESAICKNVKQMLDELEFG
                     RKSGFPYNLLRYIEQHHAEIASQFTAIFPDLAAETRQQLLSYLQGAPGQRSLVQRIEE
                     ALKLLVEDRKSLRSRIDKLKRSIDKLENAPRDQNFDSDMRELTSERQALMALVNQINN
                     KQTLNFLTDEGLLPNYAFPEAGITLRSVLWRRKEGGEAREYQNTTFEYERPASTALAE
                     LAPLNNFYAGGHKVEIEQIDLKVSEPENWRICSHCNYSENIDQTGDQHKYCPKCGTPG
                     WADAGQKTTLLKLRQVYARASARDSQISDESDSREPAFFQRQLLVSFEKEDVSAAYAI
                     EEGEVPFGFEFLSKVTLRDINFGKMADDANELMIAGEAKKRTGFKVCLGCGMVQRPRD
                     HEPRHDLSCKYRTEPEKAKFEDYLYLYRQLESEALRILLPVSSYSNDRVVEASLGAAI
                     QLGLKHYFKGNVDHLKGVVYREPENEGESWRQYLVIYDTVPGGTGSLKELMRTPDNLL
                     KLLELAYQAIVECSCNHDTHKDGCYRCVYAYRDRGRMKYVSRDQARLLLAKILQARDS
                     IRVIDSIKNISLEAMMGSELEKRFISCLQENKNLIVSRSYAHQGAGWIINTRSEPMMS
                     WHLKAQVDLGAKEAVGIPCRPDYVLYPLMQPDAIKPVAIFLDGFAFHKNSVAEDVQKR
                     QAIRDSGNFWVWTVTWADLKEPGLKHVQDVLGLGHNPDMKQPKFYNLFHDTNFAALEA
                     SFRERNSFALLLDYLADPGGRTQLWQKMAAAHAWVWLDVKKSQDAGTKQKYAYEMQEN
                     APAWRLAELLPDEPFVFGGLLDSCNSSQQFIELASVLPQQAIKPTTSVAQMRSWLRLH
                     ICFDDRYTQDDGYEAGLNGFWRLVNLLQFLPDITFTSRKAVPLSQESVDTKVSATPEA
                     VPETDESWAEIIEFGLLSAEEIALLQSHSLSAPTLGYELQNDEGEIIAEADLAWPSQK
                     WALIIDNQEFIPLFTTRGWHVAFGPIDENTLQHLSGGDK"
     gene            21401..23515
                     /locus_tag="IU325_11910"
     CDS             21401..23515
                     /locus_tag="IU325_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP5809728.1"
                     /translation="MTVQTPKVALSDGFLGAFARIPKAQQKKVQEFISKFRQDPTSNG
                     LNYEKIHDARSKNVHSVRIDQTYRGIVLKPEQGALYMLMWVDKHDEAYDWARRHDCSI
                     HPVTGAIQVIDISYIKPAAETVLDKPKLFAAYSAEQILALGVPPVFIDQVMALTDEAG
                     LNQLESIMPAEAWEPLHWLAEGLDYQEVLEEFNDHRDEPVDTNDFMEAIERSKRRFHV
                     VENEQELLQMLNAPLEKWRVFLHPSQRKLVESPANGPVRVLGGAGTGKTVVAMHRARW
                     LSQRLADKPGKKVLFTTFTRNLAADIRANLQRLCTREEMARIDVVNIDAWMSDQLKRH
                     GYDFRVVYDSDEGRRKCWNYALQQAPAELGLPDNFYAEEWQRVVQPNAVYTREEYLKV
                     SRVGRGTALSRIQRAKIWPVFEEFRAQMARAKLREMGDAMHEAIVLFKEKQVQLPYSS
                     IVVDEAQDIGAPAFTLIRSLVPESPNDLFIVGDGHQRIYRNKVVLGQCGINVRGRRSK
                     KLKINYRTTEETRQFAVGLLTGVKVDNLDGEADTSNDYLSLLHGEKPMITHAADFKEE
                     AATLVKQIQALLANQVRSQDICITARTKHACDRYASALNDAGIETFNLGNDSGDSDAR
                     PGVRVATMHRIKGLEFQYVFLVGINEGVVPEIKALASDDPVEQRDALFNERALLHVAA
                     TRAVKGLFVSSSGIPSPLLVAD"
BASE COUNT         6093 a         5660 c         6397 g         5855 t
ORIGIN      
        1 ttgctgttta ttaacggagt atattaagaa atacaaaatg aataatggct tatccttgct
       61 ggatctggat ttgttcagag gtatttcgag taattaccgc gtggttgttc agttgttaat
      121 atcatcatgt ctttctggtg atagcgatac gatttatgat atacaggacg aattaccctt
      181 ttcatgggga tggattcccg tttcaatctg gaaagatgtt tttcaaaagt gttgggctta
      241 tctggaaaaa cagattaacg ataaaacatt agcattacat atattgcaac cctttattgc
      301 ttttatgaac catcgtgcac atatcgatcg tcggctggct cccattgcga atatgttact
      361 tacatatagc gaactcctac cagccggttg taatgtcttg ccaactgtta gtcgtgagca
      421 gtttaatgaa gctaaacaga tgctattaag gaaccccgac agctttgggc gtatcagtat
      481 cttccctaaa gaactttggt ctagtgctat tactccagag ttaaaatctg tttttaataa
      541 gctttggatt gaagataaat atcactcacg gcttgaaaaa cgttttaatt tgatgttagt
      601 cgcagcgctg ttaacccaaa aagataataa cttgatatat caactgtctg cgctttttga
      661 attccactat cagcaagccc cgcagcaatt aggggtaatc tatcaatatt attttgaaca
      721 agcaggagta tgtcattgat ggacgttcag caactgcatt atgcgccact tctaaatcag
      781 cttcatcagc gcgcggtgag gtcggtggta agtcagttgg cattgcggag caagtcgttg
      841 actcattatc tggaatcaca atatttccag cctcccggtc aggaggggtc actgcttgca
      901 gacccggtat tcgaaagcac gttcggctgg atgcctgcgg atgaaacaat ggaggatttg
      961 cgggacaagc taatttccag cccgctggtt gatgcattga gcaaagcgcc ggtgccattt
     1021 cgtcatcaac ttacggcgtg gcgaacgatt ctggaagaca aaaaatcctt gttagtgagc
     1081 agcggtacgg gatccgggaa aacagaatgc tttatggtac cggttctaga agatcttatt
     1141 cgtcagcaaa aatcgacccg tcagtcacta acagggacac aagctatttt cttgtatccg
     1201 ttaaatgcat taattaacag ccaacaggag cggcttcgtg aatggacgcg cggatttaag
     1261 gggaaaatac gcttcgctct atataatggc gaaacccggc ataccaaata cgaagttcag
     1321 gaagatcagt tgaaagtacc tgaacaagct ttgagtcggg aagccattta cgaagcacca
     1381 ccttccatca tggtgacgaa taccaccatg ctggaatata tgctgattcg tcgtaaagat
     1441 gccccaatca tcgaaaagtc tcaggggata ttaaagtata tcgtcctgga tgaagcccat
     1501 agttatattg gttctcaggc tgctgagtta gcgctgcttt tacgtcgtgt tatgcaggct
     1561 ttcaatgttg gacctggcac agataaacct gtgcagatta tcgcgacctc tgccactatt
     1621 ggtgaagact ctcctgaagg taataaggag cttgctaaat ttgtcgctga tcttgcgggc
     1681 gttacagaac gtgatgtcaa agtggttcgc ggctatcgac aaataccgcg ggtatcggag
     1741 tcgctgattc gtcatgaata tcccacatct ttaacttctc ttaaatcttt gagccctcag
     1801 gatctttacc aacagctttg tcattaccgt gtagcccagc aattgcgtca ggcgttaact
     1861 catcccggac ggcaggcagt acgtttgagt gagttgctca atgttgccag aagaacctgg
     1921 ccagatatta atcaccgtga gttactgcaa ctcctcgacc taatggcgag atcgcgagaa
     1981 ggtgaactgg cttttacgcc attacgtatg catgggttta tcagaacact ggcgggtcta
     2041 tgggcatgtt caaacaaaca atgcttacat aaagcgcatg aattaaatca gagtgactgg
     2101 ccatttggtc aggtatggtt tgagcagcgc caatattgtg actgtggtgc gccggtattt
     2161 gaagttttac gttgctccgg gtgtggttca gcttatttgt ctgccaaaga ggaggtgcgc
     2221 ggtgatggaa cgaactggtt ggtagcgcaa cccgccgctg ccgaagtgga tgaatttgct
     2281 cttgatgtag atgtttattc tgaagatgaa gacaacgacg aattagacaa taactctcaa
     2341 tttgatcgtc tgattgcgat cgatggtgaa aaatatatca tttcactgga agaaacgacg
     2401 gcggggaaaa ttgatagtga agcgcaaaag cactatgaaa taaacctgtt aagaccggaa
     2461 tctcgaggtg agggacgtaa taattccttt gcttgtgttt gctgtggtga cacccaacga
     2521 aaaaataatc ctttattccg tccgcttcga cttggcgcgc cctttttttt aaatgaaatt
     2581 attcctactc tgctggagtt ttcaccatta ccgcagacaa gagaagagca gttaggaccg
     2641 tttaatggtc gacgtctgct cacctttacg gattcacgtc agggcactgc ccgcatttct
     2701 gcccgcttac agcaggatgc cgataaagcg acgttgcgag ctctttgtta ccaggagttg
     2761 gccaatatta atgagccatc agcaaagctc agcccggcac actgcgcatt gttagagagt
     2821 tttattaccc agttttctat ccttccttta aaaaactcta ttgacgcgct gaaaaaagcg
     2881 atggttgcta atgaagagct tagcccggcg gacattcagg cacttaaaat tatcgagccg
     2941 atggtgagtt ccttaagccc ggatcatgct gaagcgatgc aaatactcca ggctaactct
     3001 ggcaatcagc agaagaaaac catgtcatgg caaacactgt ctgaacagct ggttggcagt
     3061 gtggaccttg ccgatagaat gagaggcagt tttaaagaat tgtctgggct tgatcttact
     3121 aaacaacagt ttgcagattt ttgtttatac agtgagtttg gtcgccgccc taaaaatgcc
     3181 tggaccctcg aaagtatggg tctggtagcc attcactatc catttattga taaagttaca
     3241 acctgcccgc aggactggaa gacattatta cccgatcccg ataagcaatt agcggaatgg
     3301 cgtaatttat tgaagattac tcttgatttc tttatacgtg aaaacagtgc cgttttttac
     3361 gaaaacgagc aataccctcg ctggatgggg gctcgtttcc cggtaaaaat gctccagggt
     3421 ccagataaaa aaaacaaagg gcagaaacgt gatcaacttt ggccacaaat acgcgatcgc
     3481 cattataacc gggtcattaa gctattaatt gcagtctttc cggcaataca acctgagcag
     3541 gctcactgga aatcgttagt taaccactta atgattgagg tctgggatgc aattcgaccc
     3601 tgcttacgtc agtttgagtc gggttatcag ctggatatta aacaacaagc tgaattttac
     3661 agtccaaagc aagtgtggcg ttgcccctat actcgacgtg cgttagacgt cacgttactg
     3721 ggatactctc cgtatttgcc gggtagcaaa gaaattgcac ctgaaaaagc agtgttgatt
     3781 gaaatgcctg agcttcctgt acgtcactgg cgtttgtctg gtggtggaga aatcgcccga
     3841 gaagaacgtc tggagtggct ggaaagtaat gcgctgattc aacatgcccg cgaggaagga
     3901 ttatggtcta ctcgcagcga ccgactggcc ctgaaagata gctggtatcg tctggaggaa
     3961 cactctgcgc agagaacgcc tgagcagaac caatttaatg aaaaacaatt taagtcaggc
     4021 aaagttaatg tgctgaactg ctccacgacc atggagatgg gggtggacat tggtggtatg
     4081 agtttggtgg caatgaacaa cgttcctcca gctccggcaa attatttgca gcgtgcaggc
     4141 cgcgcgggtc gacgtggaga gagtgcatct gctgccatta cgttatgtaa aaacacggcg
     4201 catggaatgg aggtattcaa agaccccctc tgggcgttca acactactgc cagtgcgcca
     4261 cgggttcgtt ttgggagcag cagtattgtt caacgacatg ttaatgccct ggttttaggt
     4321 ctgtttttac gagctgaagt tcctgatgcg acaaaactga gttgcaaatg gttctttgaa
     4381 ggtgatgaga gccagtgtct tcgttttctc cattggttaa ataaccaggc cgatcagctg
     4441 gcggataaac ttaaacgcct tactcaggga accgtcctga tgtcattgac ggcaacccag
     4501 ctcttgaccc gcacacaagc aatgatgcag caggttgata tccgctggag aagccaactt
     4561 gcgatactgt tggaaaatat cgaagcgttg aaggcggata acagcgcgtg ggaagaaacg
     4621 ccagcaggta aagccatcgc ttatcagctt cgtgattatc gcggtgcata tctgttttct
     4681 aaactcatta gtgaagcgtt cctgccgggg catggtttcc cggttggcgt cgtcaatttc
     4741 aattatctga cggcagatga gctggaaaaa cgtcgcgcaa taaaagctac tcaggctgac
     4801 cctaatgaag ggggagaaag cttttctcgt cgtattgaaa aattgcccag ccgtgatttg
     4861 cctacagcat tacgcgagta tgctccaggt gcagatgtgg tattgggcgg aaaagtatat
     4921 cgttcctcgg gcattatgct tggcaaggta cttgccagcg ggcaggagct gtcaggcgat
     4981 catcatattc cgtggttctg gcattgtcgt aaatgtggtg cgggggcaac cagtactacc
     5041 cgtcctgtcg aatgtagtca ctgcaaagcg gatatacagc aacttgatgt gaagcgttat
     5101 ttacagcctg ttggttttgc gacagatatc cgttatcagg cgcataatga tgtatccatg
     5161 cctgcacaac ttccctggaa agatccacgt gttcttgtac cgtcttcggt gtgggtttct
     5221 ttacccgatg ctgggctagg acgttaccgt tttagccata gtggggaatt gttccacttc
     5281 agtgaagggg aatttgggca cggctacgca atttgcctct catgtggtcg ggcagaatct
     5341 caaaatcaac cccagcgtac ccctgaaaat cttaagaacc cagagaggga aaatacgcat
     5401 tacctgttgc gagggggcag taacgatcgt ctgggtagca ataaattgtg ccacggtcac
     5461 gtgcataaag atctttggtt ggggtatagc agtcgtacag atatggttga gctgcaactc
     5521 aatgatgata atggcctgtt gatccgtgat gaggttgccg cgcgttccct tgcggtcgct
     5581 ttgcgtgaag ggttagcgca taaattgggt atcgaaaata ctgagctggg cgtgacgacg
     5641 cagcaagcgc gcgatatcaa tggctatacc ggatattcca tcttcattta cgacaataat
     5701 gcgggtggtg caggttatgc agtgcaattg atcgaccatt gggctgatgt ctttaattac
     5761 gccagaaaat tgctcgattg cagttgcgat aaattctgtc accactgctt gctcagctac
     5821 gacagtcagc attatgtcaa cagattagat cgtcaccatg cattaaccct gcttaccaat
     5881 gtacggttgc agcgacttaa cctcgcgcca gaatatcagt acttcggcga tggtagccgg
     5941 gttgaaacta accctctgag tttacgtatt gcgcaatgcc tgaacagcga gatttatgac
     6001 tcctgctcgc tggttcttgc ggggccgcag gaacagtggg actttgccca atggccatta
     6061 tttaaagagt tgctccagtt tgcctcctcc ggtgggaatg ttgagttgct ggtggccacg
     6121 ccgctggcaa acttaactga tagctccaga catcagcttt ccgcgttggc ggcgatgccg
     6181 ggaggtcgct tacaagtgaa atccattgcc actgcgcagc tcatgcaagg aaaggggcgc
     6241 tggcttgcgc aggtgactcg cgaagggcaa agccaacagt gggccgcaga cgacagcgct
     6301 accgttgcac ctggtgaatt atggggacaa agtgcgtctt caccagtagt gacacttaaa
     6361 ggaacgtccg gaaaaacctt ctcgggtcag accttaagtg cagaagattt actgcccgcc
     6421 ttacccaccg gggcggtgcg catcaatctc tgtgaacaac tggacgggcc gctggaaggt
     6481 tttggttcgc gtttctggtc gttagtcacg caacagcacg ctggctggaa gcaggcattt
     6541 acccgccaca aagagataac gcatgttgag tacagcgatc gttatcttaa tagcccattc
     6601 acggcgcgtt tgttaggtga aatcttaacc gagctggttg aacagggaat ggcggaacgc
     6661 gccagcctca ctgtttgcgt caaaaaattg gattacaact ctcgccaaca cgacgctctc
     6721 tataacgcgt ggcttaatga ggaagatcgt cagcaggttg tgaccacttt acttgaagaa
     6781 ggatatctgg ggcctgcgtg gccgggagca attagctggt taacggggga taaccagagt
     6841 acggagcatg gacgagaact gacagtcacc ttcagcgatg gcagtcagca ttatgtcctg
     6901 ctcgacatgg ggcttagcta ctggcgctgt attgaagaca ccttctttga ttttgctctg
     6961 cgagtaccgc agcaagttga acggctggca aacacgcgag cgcgagctgt tgcctcgggt
     7021 aatgacctta ggagttatgt tatcgctgga taaataattg ctcccctggg gatatcttcc
     7081 aggggagttg acccagctca ctttttaata atttttgttt gaaaatcatg cgcaatttat
     7141 gcgcattccc tcccattttg tcactttgcc gggggccttc atgatatctt taaggccctc
     7201 ttttttatcg cagtgtgccg cgccacattt ttcaggatga cgttaccgcc actgccccaa
     7261 atagacagaa aaaccgatga ataagcaaaa ttatgcacct ggtatgcggg tggttattcg
     7321 tgatgccgaa tggcgtattc gccgggcgga tgacagtggt gatggtgggt atttgctgac
     7381 ctgcgatggt atttcagagc ttgtgcgcgg taaagaaggg ttatttctga ccaaactgga
     7441 acaaaaagta gagatcctgg atcccgcaaa aacgcatttg gtggaagatg aatccgccaa
     7501 ttatcaggcg gcgcagttgt atatcgaaag tcagttacgc caacgcgtac cgacagacag
     7561 taaagtccat tttggtcatc tggcggcgat ggattccatg ccgtttcagc tcgatcctac
     7621 gcggatggcg ctggcacagc cgcgccagcg gatactcatt gccgacgctg tcggtttggg
     7681 taaaacgctg gaagccggta ttctggtttc ggaactgatc cgtcgtgggc gcggaaaacg
     7741 tattctggtt ctggcggtta aatcgatgct gacacaattc cagaaggagt tctggagccg
     7801 ttttgcgatc ccattgaccc gcctggattc tgctggttta caaaaggtgc gtaaccgcat
     7861 ccctaccaac cacaacccgt ttcactattt tgacaagacc attatctcca tcgatacgct
     7921 gaaacaagac attgaatatc gtcaccatct ggaaaatgcc tggtgggata ttattgtcat
     7981 tgatgaggcc cataacgtcg cagaacgtgg aaccagttcg ttacgcagta aactggcaaa
     8041 gctgctcgcc gggcgtagcg atacgctgat tatgctttca gcgacgccgc atgatggtaa
     8101 ggctgaaagt tttgccagcc tgatgaacat gcttgacccg acggcgatcg ccaacccgaa
     8161 agagtatgaa tacgccgact ttgccgataa aaatctggtt gtccgtcgct tcaaaaaaga
     8221 cgtgaaagat cagatgtctg gcgagttccc cgagcgcaat atcgttaaac tgactcgcct
     8281 ggcatccggt gccgaagaag aagcataccg tcgcctggtt gagagtcagt tccgtgatga
     8341 tgacgatgaa caagcccagt ctaacaaagg tcgtctgttc aagataactc tcgaaaaagc
     8401 gctattttcc agcccgatgg cgtgtgccag cgtagtggct aatcgtctga agcgtcttga
     8461 gagccgtaaa gatcataata gccagagcca gatcaacgaa ctggagtcat tgctgttggc
     8521 gctgaataac attgatgcca gccagtttag caaataccag ttgctgctcg acaccatccg
     8581 aaaagacctc gcctggaaag ccaataacac ggaagatcgc ctggtgatct tcaccgaaag
     8641 tattaaaacg ctggagttcc tcgaacagca actgcgagcg gatctgaaat tgaaagatga
     8701 tcagatcgcc acgctgcgcg gcgatcaggg cgatacggta ttgatggaaa ccgtagaagc
     8761 ctttggtaaa acgcagtcgc cgttgcgtct tctggtttgt tcggatgtgg catctgaagg
     8821 gatcaacctg catcacctta gccacaaaat gattcacttt gatattccgt ggtcgttgat
     8881 ggtattccag caacgtaacg ggcgtattga ccgctatggg caaaaacatc agcctcaaat
     8941 ccgctatctg cttactgagg ccagcgaacc acagatcaat ggcgatatgc gcgtactgga
     9001 agtgttgatc aacaaagacg aacaggcgca gaagaacatt ggggacagtt cggagtttac
     9061 cggcaaattt acccaggaag aggaagaaga gcaggttgca gagttcatga tgcaggatga
     9121 tggtgccagc ctgtttgatc aactgctgaa cagcaacgtc tcggaaagcg ccgaacacga
     9181 tctgtttggc gaaatatgca gtgcggtttc cagcgatgcc tcaatggtca tcgaaacaga
     9241 caccagcttg tttgccagcg aacaggcgta ctgtgaaaga gcattgggtt acctgaaagc
     9301 cagtggtcaa actatccagt atgaaacctt gcctgataat accttgtcgc tggtggcacc
     9361 ggaggagtta cgccgccgct tcaaccagct accgcctgaa atcgccccgg agaactggca
     9421 gctcttttta agtcaggata aaaccgtcat caccgacgcg attgcacgcg ctcgcggtga
     9481 gcaacatgcc tggcccgatg tgcagtatct ctggcaaatc aacccggtag tgcagtggct
     9541 ggacgataaa atctcttcgg cttttggtcg tcatcaggca ccggttatcc gtctgccata
     9601 cctgcttgaa cccgatgaag atcacttcat tctttccggg ttattcccga accgtaaatc
     9661 acatccgatg gtgaacccgt ggatagtggt gagctttaac cgtgaatcgc taatcggcag
     9721 tcagccattc gctgagtttt tacaacgtca tctgcagttg agtaacaagc tgactaacag
     9781 cggcggtaaa gatcgtaatc accagcgcca gcaggattta ctggaagcgg ctattgctca
     9841 tgcccgggag gtattcatcc atgatcggaa tgcgtttgaa acgcacatta accagcaact
     9901 gaatgagcat ctgcaaaagc tggacgtttt gcgtgggcgg cagttgagcc aacttgagct
     9961 ggattttgcc gataacaaac agcaattgtc agttaagcag agccgtaaag agcagagaca
    10021 acgcgaaatc gaacacaatt ttgacagcta tatcgaatgg attgaggaca ccatgacgac
    10081 tgaaaaagaa ccctacattc aggtaattgc tgttatcacc ggagcggagg gttaatcatg
    10141 gcgctggtcg gtattaataa cgaaaacgaa ttttactcta accactattt gggtgaggta
    10201 ttcaccagtg atatccgcga tgtgctggaa ccctggatag cccaggaaaa tgcagcgcgt
    10261 gaagcggagc gtgccgctcg tgaacagggc aaagacgtag agccgggata ccgcgctccg
    10321 tggaaccagt ttaacagtct ggcgactgag tttttccgca aacttgccga gcacgaaaaa
    10381 cagcgtcaga tcccgcagcg tctggccgat caacgtaatc gctggcagcc attgttaaag
    10441 gcgctgggct acgaaattac gccgcagatc caaatgctgg atgacgatac accactgccg
    10501 gtactggcgc gttacaacag cactgacggt agcccgtggc tgtggattgt tgaagcacac
    10561 gatcaggaag aaggaacgct ggatccgctg gcgctctctt tactgaccgc gcaattcccg
    10621 gcggataccg acaaacataa gcgcgacagc ctgcgcaaaa aagccaacgg tgaatatcgc
    10681 agctggcagg atctgctctc tacggcggtc ttcactcaaa atgaaccgcc gcgttttgtg
    10741 ctgctgctcg gtaaccgtca gctattgttg ttggaccgta ctaagtgggc gcaaaaccgt
    10801 ctgctacgtt ttgattttga agagatttta agtcgtcgtg aaacggatac gctgaaagcg
    10861 acggcagtgt tgctacataa agattcgctg ctgccgggca gtggggcacc ttatcttgac
    10921 tcgctggatg acaattcgca caaacatgcg tttggtgttt cggaagatct gaaatatgcc
    10981 ctgcgcgaaa gtattgagct gctgggcaac gaagcgatgc attatctgat cgaccgtggc
    11041 ctggcaaact ataccggtaa ccgtgcggtg gacccggatg aactgagccg cgaatgtctg
    11101 cgttacatgt accgcctgct gttcctgttc tacattgaag cgcgcccgga gctgggttat
    11161 gcgccaatga ccgccaaaac ctatctgcaa ggttacagcc tggaaacgtt gcgtgatctg
    11221 gagatgatcc cgctgaccag cgaagaagat cgcaacgggc gctacttcca cgacagcctg
    11281 aatatgctgt ttaaactggt gcgcgaaggc tacaacggcg gcgtgaaaat gcagagtgac
    11341 ctggagagcg gcgaccggat caccatccat agtcatcagt tcagcgtccc gcgtctggaa
    11401 agtcatctgt ttgatgccaa caacacgcgc attcttaacc gcgtggtatt ccgtaacgaa
    11461 accctgcaac agattatcca ggcgatgtcg ttaagccgcc cgggcaaagg gcgctttaac
    11521 cgccgcggac gtatttctta tcgccagttg ggtatcaacc agttgggggc ggtgtatgag
    11581 gcgctgctct cctatcgcgg attcttcgcc agcgaagatc tctacgaggt gaagaaagcc
    11641 ggggaagagt ttaacgagct ggagacgggt tacttcgtca gtaaggatga gattggcaaa
    11701 taccacgaag acgagaaggt ttacgagaaa gacggcagtc tgcgcattca ccgcaaaggc
    11761 agttttatct accgtatggc cgggcgcgac cgtgagaaat ctgcctccta ttacaccccg
    11821 gaagtgctga cccgttcact ggttaaatat gccctgaaag aactgtttaa ggagcagatt
    11881 gatcccatta gcgatccgca cgccaaagct gatgccatct taaacctcac tgtgtgcgaa
    11941 ccagcgatgg gcagcgcggc gtttcttaac gaagccatca accagctggc ggaagcgtat
    12001 ctgttccaca agcagcaggc ggaaggtcgc cgtattccgc aggatcgtta cacccaggag
    12061 ttacagcggg tgaaaatgta cattgccgac aacaatgttt tcggcgtgga cttaaacccg
    12121 gtggcggtgg agctggcgga agtgtcgctg tggctgaacg ccatcagtgg cgatgccttt
    12181 gtaccgtggt ttggttacca gttgcactgc ggtaactcgc tggtgggcgc gcgccgtcag
    12241 gtgttcaaca agagcgaact gacctacaaa aaagccaaag atccgagctg gcttaacagc
    12301 gaaccggtcg agctggcgat gaacacgccg cgtgaagaga cgcagatttt ccacttcctg
    12361 ctgcccgacg gcggtatggc taactacagc gataaaactg ttaagcagcg ttatccggat
    12421 gacttcaaag ccctggacag ctggcgcaaa gagtttatta aaagctttgc cgggcatgag
    12481 attgctgatg tgcagcgtat cagcgaaaag gtggaagcac tgtggaacac ctatcgccag
    12541 caacttaaag cagaacgact gaaaaccgcc gacagctacc cggtgtggcc ggcagaaaac
    12601 accgagcaga cgcgttcttc gctgagcagt aaagatgaaa ccttcagtgg tcgtcttgaa
    12661 gataacagtg cctaccagaa gctgcgttgg gtgatggact actggtgcgc gctgtggttc
    12721 tggccgatcg acaaagccga tgagctaccg gatcgcggca cctggttgtt tgagattgaa
    12781 accctgctcg acgggattgt aatcacggaa aaagtcactg aagttgcgga gcacaccact
    12841 ggcgatctgt ttgccgaaga cggcctgctg cgagaagagt cttcgctgtt ttccggcgca
    12901 ggtcgtctga aaactgaggt gttgttccgt catttgccgc gtctggcgat tgtcgatgcc
    12961 ctgagaaagc agcaccgttt cttccactgg gatctggagt tctgcgacct gtttgccgag
    13021 cgcggcggtt ttgacctgat gctcggaaac ccgccgtggc tgaaagtgga atggcaggaa
    13081 gcgggcgtgc tgggtgatta cgagccggaa tttgtgctgc gtaagctgag tgcatcgaag
    13141 ctggcaacgt tgcgtattga tacctttaac cagatcccgg cgctggaagc ggcctggcgc
    13201 agcgagtatg aaggctgtga agggatgcaa aacttcctca atgcgcagca gaactacccg
    13261 gtactgcgcg gggtgcagac caacttgtat aaatgcttcc tgccgcaagc atggcgctta
    13321 ggggcgcaga aaggcgtgtc aggtttcctg cacccggaag ggatttatga tgacccgaaa
    13381 ggcgggctgt tgcgtgcggc agtgtacccg agactgaggg cgcattttca gtttgcgaat
    13441 gcttatttat tattcaatga agtacatgat caaactcggt ttggtataaa tgtttatggg
    13501 ccttaccaac aaagtattag cttcgtttcg atggctaatg tgtttctccc tcaaactata
    13561 gatgcttcat ttgcgcacaa aaattactct gaacaggttc cggggattaa gtttgatagt
    13621 gctcttgagg caggctcatc ggttctatgg aataataatg gtcataattc tagacttata
    13681 aatgttggtc cgaaagaact tacgttattt gctcgtctgt atgacagcga aggaacgcca
    13741 gcctggcagg cacgtttgcc agccttacat gctaaacaac ttgttgctgt gctggaaaag
    13801 tttgctaacc agcccaagag attaggcgat ttacaggggc aatatttatc cctggaaatg
    13861 tggcatgaaa caaaccagca gaaagacggc actattgaac gtaagacgca gttccctgag
    13921 gatgcatcac aatgggtatt gtctggcccg cacttctttg ttgggacgcc gttctacaag
    13981 actccgcgcg aaaactgtac gcttaacagc gattatgact gcctggactt gctaactctg
    14041 cctgacgatt acctgccgcg cactaactac attccggcat gtgatgcaca ggagtatgca
    14101 aagcgcaccc cacgggtttc gtggaaagag caggatgaag acgagccgag gaaggtgacg
    14161 gattattatc gttttgttaa tcggagaatg tttggtggat cttctgagag atctttcatc
    14221 tccgctattc ttcccaaggg atttgcaaac attaacacat gcgtcgctac ggcctttgag
    14281 tcaactaatg tactggtgaa ttttacgggc ctttcgcatt caattgttta tgattttatt
    14341 ttgaaatcaa ctggaaaatc agatctgtac ggtgcacaac ttggtgggtt cgtatatacc
    14401 gaaagttttg gtgtaaaaat aaggacttta ttattaaatg ccctatcaag tgaatacgcc
    14461 gagctctggc aatcctgcta caccccagac ttcaacaccc agcgttggag ccgcgatctc
    14521 ccgcagctcc cccaggattt cttcgccaaa ctgaccccag agtggcagcg taactgcgcc
    14581 ttacgctccg actatagtcg tcgtcaggcg ctggtggaaa tcgacgtact ggtggcgcag
    14641 tcgctggggt taaccctcga agagctgctt accatttacc gtgtacagtt cccggtgatg
    14701 cgccagtacg aagcggatac ctggtacgat caaaacggtc gcattatctt taccccaagc
    14761 aaagggctgg tgggcgtcgg tctgccgcgt accgcccgta aagctgacct gaaaaacggc
    14821 tttgtcttta acgtcgacag cccggagtgg accggcggtg actgcaccga tcaagctatc
    14881 ggttgggatg atgtcaaaca tcttaaaacc ggtaccgtca gcgtcacctt tgatgactat
    14941 acccgcagcg acgaaggtga gcgccgtacc gtcacctggc aggctccgtt tatcaagcca
    15001 gatcgcgaag acgactacaa agtggcctgg gcgttctttg cacaagataa ggagagcgcc
    15061 tgatgctgcc gtccgttgtc agtcggcagg tcgccgacag cgttgccgcc tttttacggg
    15121 cggcgttccc gctaaacagc ccgctgttta atggtgaaga aaacaataac gtctcgatgc
    15181 tggagcaatt tctggcccag ccggaaacgc tgctgaaagg gccgtatctt tccgcccagt
    15241 tgccgtttcg taaaagtgac ttaccgctga attttttccc taatctgacg ctgccgttcc
    15301 cgccgcacgc gcatcaggcc cgggcatttc agcgtctggg aagcgacgcg ccacagccga
    15361 cgctggtcgc taccggtacc ggctccggta aaacagaatg ttttatgttc ccgctgctca
    15421 accactgcgc gggcgcgtca caagccgggg ttaaagcgat tatcatctac ccgatgaacg
    15481 ccctggcaac cgatcaggcc agccgtttcg ccaaaaccat cgccagcgat ccacaactgc
    15541 atggcaaggt gaccgtcggc ctgtttgtcg gcgacagcga aatcgaaccc agcaaaaaaa
    15601 tgtctgcaga aaaggtcatt acctgtaagc acacgctgcg cgaaagcccg ccggacatcc
    15661 tgttgaccaa ctacaaaatg ctcgactacc tgctgatgcg ccctggcgat cagccgctct
    15721 ggcgttacaa ccagcccggc tcattgcgtt acctggtcgt ggatgaattg catacgtttg
    15781 atggcgcgca agggtcggat ctggcctgtc tggtgcgacg tctgaagcat cacataggtg
    15841 tggataacca acaattcgcc tgcgtcggga catcagctac cgtcggcgac gaattggggc
    15901 aactgctcga ttacgcaaaa acaatctttg accagccgtt taccgatgac gccgttattc
    15961 gcgaagatcg ctacacggca gcggagtact tgcaggatta caccattgcg tacagtcagt
    16021 atccggggcc ggaggtcgct tcggccctgg atccgcaggg ttacgctacc ccggtggcgt
    16081 atctcaacgg acaaatccct ttgtggttcc ccgacagcga cttgcaactc ccggacgatc
    16141 tggagagcga cgacgggcgt gaaaagcgta ttgcgctggg gtcactgtta cgccgccaca
    16201 gcgtgatgca tgtcttactc catgatctgc aaggcgggat cctctctgaa cgagagtgtc
    16261 tggaaaatct ccaggtcatg ctggcagaaa gcgccgacca tgcccgacgc gtgctgcaaa
    16321 gcattctggc gctgatcgcg caggcacgtc tcgaagtacc ggaaacggaa cacgatcggc
    16381 aaaaacgtct ccaggcgaag aaagcgcgcc cggtgctgcc gtttgtgcaa ctgcgttccc
    16441 agttgtggct tcgtgagatg cgtcgtctgg tcgccagcgt ggcgaaaacg ccgagactgg
    16501 tgtttgccga tgatgtggcg gtggaagatc gcgaacacta tttgccggtt atccactgcc
    16561 gtgactgcca cgcaaccggc tgggccagcc tgcgtcatgg gcaaagtgtt cagctggaaa
    16621 cagacctcga cggcatttat cgccagttct ttgaaaaagg gcgctccatc gtcctcgctt
    16681 tcccggataa taacgaaaaa gcggtcagtg gcgcacacca gaaactgtgt cctacctgtc
    16741 tgaaacttaa ccgacagtcc agcgtccagt gtggacattg cggtcataca gagctgttgc
    16801 aggtactgat cccggatatg ctcaaagagc gtaaggacga gctggagttt gctaatgaat
    16861 gcccgtactg caacagcaaa caggggattt cgattctggg gtcacgggct gccagtctgt
    16921 cggcggtgat gattcaccag ctttatggca gccagttcaa cgaacataaa aagctgatta
    16981 cgttttctga ctcggtacaa gacgccgcgc atcgtgccag cttctttact gcccgtacct
    17041 ggccgctgat gattcgtggg caaatcgcca gtgttcttgg cgaaaaagag acattaccgc
    17101 tggatgcgtt cgcggcgcgt gtttgccaac aaacgcgtga acgttcaccg gacgatgctc
    17161 attttgcagc gaacttcatc gcaccaaata tgcagtggct gaatgattat cagacgctga
    17221 aaaatgaagg caaactggcg gagaactccg atctgccgga gctggtttcc ctgcgcctcg
    17281 actgggaggt gtacagcgaa tttaccctgc gcgcgcgtat cggacgtacg ctggaagcca
    17341 ccggttatgc cgttgccgga gtggataaag atcgcttcaa tccggctatt accgccctgc
    17401 atcaacagct ttgtgaagag ctgggtgatg agatgtccgg tgtgagcgtc gagcaggtcg
    17461 cacacctggc gctgggggtt atctggtatc aacgccaggc cggggccgtt atgcatccgg
    17521 cgttcgaaag ttatcgccgt gatggcacga cctttatgat gacgcaaaaa gagcgtacct
    17581 cgcgctatat gccttctatc ggtccgaaaa cgcgtaaacc ggcttatgcg tcgtttgaaa
    17641 aaattaatgg tttccatcgc ctgattggcg cgaagtctaa ccccagttgg tatcagcatt
    17701 ggatcaatcg cacgctgagt aacggcaata atctgtttat cagtagcgtt gcggaaacag
    17761 tgttaagacg cctgtttacg gcgttgaaaa tagccggagt agtaaaagat tttgacacca
    17821 aagggcgtga agcctgggga ctggtgcctt cggcgctggt ggtgagccgt gacgtcgttc
    17881 agcttaattg ctcatgttgc cgtgaggttg tccgggctcc ggccgatcaa ggctggcagt
    17941 ggcgtaatgc gccttgtctc tctttacgtt gtgaaggtcg ttaccaggaa gtcgacaata
    18001 cggcgacctg gcgttgggag aacatggata tcgcgcgcgt gcagggcgca gaacacacgg
    18061 ggctgcttag ccgtgaagat cgcgaagcca ccgaaaaatc gttctaccgc ggcaaccaac
    18121 cgtggaatat caacctgttg tcggcaacac cgacgctgga gatgggtatt gacgttgggg
    18181 atctttccac cgttttactc tgttctgtcc cacctgctca ggccaactac ttgcaacgta
    18241 ttggtcgcgc cggtcgtaaa gatggtaacg cgttaaacat taccgtggca gaagggaacc
    18301 cgcacgatca gtttttcttt gaagaaccac tggagatgat gcagggccag gtgcaggctc
    18361 ccggcgtctt cctcaatgca acggctattc tcgaacgcca gctggcagct ttttgtatgg
    18421 ataactgggt gaaaaccggt gtaccagaat ccgctatctg taaaaacgtc aaacagatgc
    18481 tggatgagct ggagttcggg cgtaaatcgg gttttccgta taacttactg agatatatcg
    18541 aacagcatca tgcggagatt gcttcacaat ttacggcgat cttccccgat cttgccgcag
    18601 aaactcgtca acagttgttg tcttatcttc agggcgcgcc aggacaacgc tctctggtgc
    18661 agcgtatcga agaggcgtta aaactgctgg tggaagatcg taaatctctg cgttcacgca
    18721 tcgataaact caagcgcagt atcgataagc tggagaacgc gccgcgcgat caaaactttg
    18781 acagcgatat gcgcgagtta acttcagaac ggcaggcgct gatggcgctg gtcaatcaaa
    18841 tcaacaataa gcagacgctg aatttcctca cggatgaagg tctgctgcct aactacgcct
    18901 tcccggaagc cggtattacg ttacgttctg tgctctggcg gcgtaaagaa ggcggggaag
    18961 cgcgtgaata tcaaaacacc actttcgaat atgaacgccc agcatcaacg gcgctggctg
    19021 agctggctcc gctgaataat ttctatgccg gtggacataa agttgaaatt gagcagatag
    19081 acttgaaggt ctctgagccg gagaactggc gtatctgtag ccactgtaac tacagcgaaa
    19141 atatcgacca gacgggcgac cagcataaat attgcccgaa atgtggcaca ccggggtggg
    19201 ccgacgccgg acaaaaaacc acgctgctga aacttcgcca ggtgtatgcc cgcgccagcg
    19261 cacgtgatag ccagatcagc gacgagagcg acagccgtga acctgctttc ttccagcgtc
    19321 agctattggt tagtttcgaa aaagaggatg tatcggcggc atacgcgatt gaggaaggcg
    19381 aagttccgtt tggttttgaa tttttaagca aagtcaccct gcgggatatc aacttcggca
    19441 aaatggcgga tgatgccaat gagttgatga ttgccggaga agcgaagaag cgaaccgggt
    19501 ttaaagtttg cctcgggtgc ggcatggtgc agcgtcctcg cgatcatgaa cctcgtcatg
    19561 atttgagttg caaatatcgc acggaaccgg aaaaggcgaa gttcgaagat tatctctatc
    19621 tctatcgcca gctggagtca gaagcgttgc gtattctgct cccggtaagc agttacagta
    19681 atgaccgtgt cgtggaggct tctcttgggg cggcgatcca gctcggcctt aaacattact
    19741 tcaaggggaa tgtcgatcac ctgaaaggcg ttgtgtaccg cgagcctgaa aatgaaggtg
    19801 aatcctggcg gcagtatctg gtgatttacg ataccgtccc ggggggaact ggttctctta
    19861 aagagctgat gcgcactccc gacaatttgc tgaaattact tgaactggcc tatcaggcaa
    19921 tagtggaatg cagttgcaac catgatacgc ataaagatgg ttgttatcgt tgcgtttacg
    19981 cttaccgtga ccgtgggcgc atgaagtatg tctcccgcga tcaggcacgt ctcttgctgg
    20041 cgaaaatttt gcaggcaaga gattcgatac gggtaattga ttccatcaaa aacatttccc
    20101 tcgaagccat gatgggcagt gagctggaaa aacggtttat tagctgcctg caagaaaata
    20161 aaaacctgat tgtcagtcgc agttacgcgc atcagggcgc tgggtggatt attaacaccc
    20221 gcagcgaacc gatgatgagc tggcatctta aagctcaggt ggatttaggc gctaaagagg
    20281 cggtggggat cccttgtcgc ccggattacg tgctatatcc gcttatgcag cctgatgcca
    20341 ttaaaccggt agctattttc cttgatggct ttgcgtttca caaaaatagc gtcgcagagg
    20401 atgtgcaaaa acgccaggcg attcgggata gcggtaattt ctgggtgtgg acagtcacgt
    20461 gggccgatct caaagagcct gggcttaaac atgttcagga tgtcctgggg cttgggcata
    20521 acccggatat gaaacagcct aagttttata acctgtttca cgataccaac tttgcggccc
    20581 ttgaagcgtc gttccgggaa cgcaacagtt ttgcactgct gctggattat ctcgccgatc
    20641 cgggcggaag aacgcagtta tggcagaaaa tggctgctgc acatgcctgg gtgtggttgg
    20701 atgtgaagaa gtcacaggac gcgggaacga aacaaaagta tgcctatgaa atgcaggaaa
    20761 acgcgccagc ctggcggcta gcagagcttt tgccagacga accgtttgtc tttggtggtt
    20821 tactcgatag ctgtaactca tcgcagcaat ttattgagct ggcttcggtg ttgccacaac
    20881 aagccattaa accgacgacc agtgtcgcac aaatgcgcag ctggctgcgt ttgcatatct
    20941 gttttgacga tcgctatacg caggatgacg gttatgaagc gggactcaat ggcttctggc
    21001 gactggtaaa cctgttacag ttcctgccgg atataacctt taccagccgt aaagccgtgc
    21061 ctttatcgca ggaaagcgtt gacactaaag tttcggcaac gccagaggca gtaccagaga
    21121 ctgatgaaag ttgggctgag attattgagt ttggcttatt aagcgctgaa gagatcgcgt
    21181 tgcttcagtc tcattctctc tctgcgccga ctctcggcta cgaattgcaa aatgatgagg
    21241 gtgaaattat tgcggaagcc gatctcgcct ggccatcaca aaaatgggca ttgattattg
    21301 ataatcaaga atttattcca ttattcacaa ccaggggttg gcacgttgca ttcgggccaa
    21361 ttgacgaaaa tacattgcaa catctgtccg gaggtgacaa atgacggtgc aaacgcccaa
    21421 ggtggcactt tctgatggtt ttctgggcgc attcgcgcgg atcccaaaag cacagcaaaa
    21481 aaaggtgcag gaattcatct ccaaattccg tcaggacccg accagtaatg gacttaacta
    21541 tgaaaagatc cacgatgcac gcagtaaaaa tgtccattcg gtgcgaatcg atcaaactta
    21601 tcgcggcatt gttctgaaac ctgaacaggg tgccctgtat atgctgatgt gggtggataa
    21661 acacgacgaa gcatacgact gggcgcggcg gcatgattgt tcaattcacc cggttaccgg
    21721 cgcaattcag gttattgata ttagctatat caaaccggcg gcggaaacgg tgctagataa
    21781 accgaaactc ttcgcagctt acagcgcgga acagattctg gccctgggtg tgccgcctgt
    21841 ctttattgat caggtgatgg cgcttacaga tgaagccggg cttaatcaac ttgaaagcat
    21901 aatgccagca gaagcctggg aacccctgca ttggctggca gaagggctgg attaccagga
    21961 agtgctggaa gagtttaacg atcatcgtga cgagcctgtt gataccaacg actttatgga
    22021 ggcgattgaa cgaagtaaac gtcgcttcca tgtcgttgaa aatgaacagg agctgttgca
    22081 gatgcttaat gcgcctttag aaaaatggcg tgtatttctg catccgagcc agcgaaaact
    22141 ggtggagtcg cccgctaatg gtccggtacg agtactgggc ggtgcaggta cagggaaaac
    22201 ggttgttgca atgcaccgtg cgcggtggtt atctcagcgt ctggcagata aacccggtaa
    22261 gaaggtactt ttcacgacct ttacgcgtaa cctggcggca gatattcgcg ctaatctcca
    22321 gcggttgtgt acgcgagaag agatggcgcg tattgacgtc gttaacattg atgcctggat
    22381 gagcgaccaa ctaaaacgcc acggttatga tttccgcgtg gtatacgaca gcgatgaagg
    22441 gcgacgtaaa tgctggaact atgccttgca gcaggcacct gccgaactgg ggctacctga
    22501 caatttctat gctgaagagt ggcaacgcgt tgtccagcca aatgcggttt acacccggga
    22561 ggaatacctc aaagtcagcc gggttggacg tggtacagcg ttatcccgta ttcaacgggc
    22621 gaaaatctgg ccagttttcg aagaattcag agcacaaatg gcgcgtgcca aactgcgcga
    22681 aatgggcgat gccatgcatg aggctattgt tctgtttaaa gaaaagcagg tccaactacc
    22741 ttatagcagt attgtcgtcg atgaagctca agatatcggt gccccggcat ttactcttat
    22801 tcgcagcctg gttcccgagt cgcccaacga tctgtttatt gtcggggatg ggcatcagcg
    22861 tatttatcgc aataaggtcg ttcttggtca gtgtggtatt aacgtccgtg gtcgtcgcag
    22921 taaaaaattg aagattaact atcggaccac agaagagacg cgtcaatttg ctgttgggct
    22981 gttaactggt gtgaaggttg ataacctcga tggcgaagcg gataccagta atgattattt
    23041 gtcattactg cacggggaaa agcccatgat tacgcatgcg gcagatttca aagaagaggc
    23101 tgcaacactc gtcaagcaaa ttcaggcatt gctggcgaat caggttcgtt ctcaggatat
    23161 ctgcattact gcgcgtacta aacatgcttg cgaccgctac gcatcagcac tcaatgacgc
    23221 cggaattgag acatttaatt taggcaatga tagcggtgac agcgatgcac gcccaggcgt
    23281 gcgagttgca acgatgcacc gtattaaagg gcttgagttc cagtatgtgt tcctggtggg
    23341 gattaacgaa ggcgttgtgc cggaaataaa agcattagca tccgatgatc ccgttgaaca
    23401 gcgtgatgca ttatttaatg agcgcgcgtt actgcatgtg gctgcaaccc gtgccgtaaa
    23461 agggctgttt gtttcatcat ctggtatccc tagtccgcta ttagtggctg attgataata
    23521 tgtccgaaac agaccaggag ccgaaacgct cctggtctat ataaccgttc tggcaactgg
    23581 cgatatttat attgtttagc tcccaacagc cagacaatat cagcttagaa attatattcg
    23641 cttatgcggt tagcatattg cacagtacaa cctgttttct ctatatctaa cgtaacatca
    23701 ggatgtggat gatttttgga tggggtaaca cactggacaa atatagctgg cacgaaaagg
    23761 ctggcatttt ctggtgacca atagtctttg gaatcatgat tataaaacaa acaccttaaa
    23821 ttttaaacgt atgatgccag gattaccaga attataaacg aatgtatgag ttcttttctg
    23881 tttgttcagg ggagggtata aatattgtaa ttgttaatag tatcgtcagg acgataaaaa
    23941 tataatcatg acaaaggaaa ttgaactaaa atgattgacc ttttacaggt ggtttgtcag
    24001 tgatt
//
LOCUS       DACBVI010000062        23583 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.062, whole genome shotgun sequence.
ACCESSION   DACBVI010000062 DACBVI010000000
VERSION     DACBVI010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.062"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(299..1054)
                     /gene="mlaA"
                     /locus_tag="IU325_11915"
     CDS             complement(299..1054)
                     /gene="mlaA"
                     /locus_tag="IU325_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HAP5809729.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            1348..2280
                     /locus_tag="IU325_11920"
     CDS             1348..2280
                     /locus_tag="IU325_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAP5809730.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2356..2430
                     /locus_tag="IU325_11925"
     tRNA            2356..2430
                     /locus_tag="IU325_11925"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2389..2391,aa:Arg,seq:cct)
     gene            2640..2792
                     /locus_tag="IU325_11930"
     CDS             2640..2792
                     /locus_tag="IU325_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809731.1"
                     /translation="MHSLMNSQARDTRDRSLNDDNVLEISEPRIRSTFERLYQNMNEA
                     FGLGDY"
     gene            complement(2872..4119)
                     /locus_tag="IU325_11935"
     CDS             complement(2872..4119)
                     /locus_tag="IU325_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HAP5809732.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCIAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPIPSAI"
     gene            complement(4181..5104)
                     /locus_tag="IU325_11940"
     CDS             complement(4181..5104)
                     /locus_tag="IU325_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HAP5809733.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHAEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELESEK"
     gene            5320..6753
                     /locus_tag="IU325_11945"
     CDS             5320..6753
                     /locus_tag="IU325_11945"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP5809734.1"
                     /translation="MTQSRLHAAQNALAKLHERRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDENDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFVAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQGDMLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWQLG"
     gene            complement(6761..7819)
                     /locus_tag="IU325_11950"
     CDS             complement(6761..7819)
                     /locus_tag="IU325_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP5809735.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPKDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            7999..8217
                     /locus_tag="IU325_11955"
                     /pseudo
     CDS             7999..8217
                     /locus_tag="IU325_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            8235..9563
                     /gene="dsdA"
                     /locus_tag="IU325_11960"
     CDS             8235..9563
                     /gene="dsdA"
                     /locus_tag="IU325_11960"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HAP5809736.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRNAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAEQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMEQYLAKGR"
     gene            complement(9671..11209)
                     /gene="emrY"
                     /locus_tag="IU325_11965"
     CDS             complement(9671..11209)
                     /gene="emrY"
                     /locus_tag="IU325_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HAP5809737.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVMSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(11209..12372)
                     /gene="emrK"
                     /locus_tag="IU325_11970"
     CDS             complement(11209..12372)
                     /gene="emrK"
                     /locus_tag="IU325_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HAP5809738.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            12788..13402
                     /gene="evgA"
                     /locus_tag="IU325_11975"
     CDS             12788..13402
                     /gene="evgA"
                     /locus_tag="IU325_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HAP5809739.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            13407..17000
                     /gene="evgS"
                     /locus_tag="IU325_11980"
     CDS             13407..17000
                     /gene="evgS"
                     /locus_tag="IU325_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HAP5809740.1"
                     /translation="MKFLPYIILLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALTEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKDSVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDETGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPAAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPEHYQVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     DIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEITQQVAAVEAKAEQPMTLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAPIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILKLLNSVKEHIAELDQEIAVFCQQND"
     gene            complement(17056..18201)
                     /locus_tag="IU325_11985"
     CDS             complement(17056..18201)
                     /locus_tag="IU325_11985"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HAP5809741.1"
                     /translation="MTNNECKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(18275..19219)
                     /gene="yfdV"
                     /locus_tag="IU325_11990"
     CDS             complement(18275..19219)
                     /gene="yfdV"
                     /locus_tag="IU325_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HAP5809742.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(19288..20982)
                     /locus_tag="IU325_11995"
     CDS             complement(19288..20982)
                     /locus_tag="IU325_11995"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HAP5809743.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNDIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLVIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAATARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(21036..22286)
                     /gene="frc"
                     /locus_tag="IU325_12000"
     CDS             complement(21036..22286)
                     /gene="frc"
                     /locus_tag="IU325_12000"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HAP5809744.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPDWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(22799..23431)
                     /locus_tag="IU325_12005"
     CDS             complement(22799..23431)
                     /locus_tag="IU325_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HAP5809745.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NVNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         6742 a         5499 c         5146 g         6196 t
ORIGIN      
        1 gtgcactata agaaactcga aggtaaaatt gaagaaaggc ttcaaaattg ccagacggaa
       61 tatcaaaatg aaattaatca actatctgcg ttaggtgtta attttgatga tgtttaaaat
      121 tatttttcca gttttttgat ttttcaacga ctgaatatct gaaatagtca atgacgaaca
      181 tcagtatttt tacatcatta cgtccgggtc attgtcggtt tatctccttt tacttgtggt
      241 aattgaggga taacaacaaa aaaggtgagt tttgcgactc acctttttta tttgtttctt
      301 attcagaatc gatatctttt aaatcatcct gaatcgcttg cgcgttcgga ttttcctgcg
      361 gtttgagttc gccgccatta gcgatgaaat catgacgctg gaagtacgct tcgcgcacca
      421 taatgtaagg atcggacgac tgacgcagca gaccgtcgga atccagcagc tgtgcgcggg
      481 tttcgatacc ttcaagcgtc catttaccca cagacatcgg ccaggtcaac caggaaagaa
      541 ccgggtaaag accatccgcc atatcaccac cgtcatcacg cagcgtgaag ctaccgtaga
      601 acggtaactg aacgtatggc ccataaccca caccataatg accaagcgta ctaccgaagc
      661 ggtgaggttc agtccgttgc agtttcgggt tcgccatccc tgcaacatca ataaagccgc
      721 ccatccccaa aatggtgttc aggaaaaagc gggtaaagtg gaccatcccc tgataagggt
      781 cgccctgcaa gaagtagtta accatcaccg caggttcttc aaggttgcca gtaaagttgc
      841 tcaaaccgtt acgcgccggt tgcggaacat aatcacgcca ggcgacagcg actggtcgaa
      901 caatatacgg gtctaataca ttgaagttga agttgtacat ggtgcggttg aacccttcta
      961 acgggtcaga acgcccttgc tgatctgtac cggaactcgc acaccccacc agaagcgtag
     1021 ttcccagagc aagcgccgac aggcgaagct tcataaatgt ctccctgttt ttttatggct
     1081 tatgcagttt gccatccatg acggaacgat accgtatccg cctgtttagg tgtgggcgat
     1141 tgtaacagca cgtcaactga tgtccagacg ccctgatttg ctgatttgat catagcctgg
     1201 taatcgccgc cctgtaggct acttgattct atagaaacag aaaaaggcaa acgcggcctt
     1261 ttctacattt tcagagtaac tcccgccagt tgcgagcaaa aaagccgcta cgctttagct
     1321 atacgtgcta attcaagaga agagaccatg gacaacgaca aaattgatca acacagcgac
     1381 gaaattgaag ttgagagcga agaaaaagag cgcggcaaaa aaatagaaat agatgaagat
     1441 cgactcccct cccgggcgat ggcaattcat gagcatatcc gccaggatgg tgaaaaagag
     1501 ctggaacgcg acgcaatggc gctactgtgg tcagccattg cggcgggtct gtcgatgggc
     1561 gcttcgctac tggcaaaagg gatatttcat gtcgaactgg aaggcgtgcc gggcagcttc
     1621 ttgctggaga atctcggtta tacctttggt tttattatcg tcattatggc ccgccagcaa
     1681 ttatttaccg aaaacaccgt gactgcggta ctacccgtca tgcaaaaacc gacaatgagc
     1741 aacgttggct tacttatgcg gttatggggc gtcgtgctgc tgggtaatat tctcgggaca
     1801 ggtattgcgg cgtgggcatt tgaatatatg ccaatcttca atgaagaaac tcgcgatgca
     1861 tttgtcaaaa tcggcatgga tgtgatgaag aacaccccca gcgagatgtt tgccaacgcg
     1921 atcatttccg gctggctgat cgccactatg gtatggatgt ttcctgcagc gggtgcggca
     1981 aagattgtgg tgattatatt gatgacctgg cttattgcac tgggtgacac cactcacatc
     2041 gttgtcggtt ctgtagaaat cctctatctg gtgtttaacg gcacgctgca ctggagcgat
     2101 ttcatctggc ccttcgcact acctacttta gcggggaaca tctgcggcgg cacctttatc
     2161 ttcgcgttaa tgagtcatgc acagattcgt aacgacatga gcaacaagcg caaagcagaa
     2221 gcacgccaaa aagcagaacg tgcggaaaac attaagaaaa atgataaaaa cccggcataa
     2281 atggcgaggg tttaagcaat cgagcggcag cgtacttacc ccgcagtcca ttagcgggta
     2341 tactcatgcc gcattgtcct cttagttaaa tggatataac gagcccctcc taagggctaa
     2401 ttgcaggttc gattcctgca ggggacacca gatacccttc taacaatatc taccttcacc
     2461 ccgtaaaaga tgggtttggc agcacacttg ccctatatct actcattttt actgcaacag
     2521 gttgaaatct cagcactgtc agaaagcgct gatgactaaa cagccctggg ccgggcgatg
     2581 taaccatcat acagaatcct gatagcgaaa tatggcgtga ctcgatactt cactctgcaa
     2641 tgcattcctt gatgaattcg caggcccgtg atactcggga caggtcgctg aatgacgaca
     2701 atgtcctgga aatcagcgaa ccgcgtatcc ggagtacatt tgagcgactg taccagaaca
     2761 tgaatgaggc gtttggatta ggtgattatt agttgggcta agcatttttg tattattatt
     2821 ttccggttga gggatatgga gatatcgaca acaaccggaa aaagtttacg tctatattgc
     2881 tgaaggtata ggcgtttcca taactatttg ctcgcgtttt ttactcagga agaaaatgcc
     2941 aaatagcaac atcaggcaga caatacccga aattgcgaag aaaactgtct ggtagcctgc
     3001 gtggtcaaag agtatcccag tcggcgttga aagcagcaca atcccaagcg aactggcaat
     3061 ttgaaaacca atcagaaaga tcgtcgacga caggcgctta tcaaagtttg ccacgctgta
     3121 tttgaagacg gatatgacac aaagtggaac ctcaatggca tgtaacagct tcactaatga
     3181 aataatccag gggttaacga acaacgcgca ggaaaggata cgcaacgcca taatcacaac
     3241 accgataagt aatgcatttt ttggccctac ccgattcaca aagaaaggaa taatcgccat
     3301 gcacagcgct tcgagtacca cctggaatga gttgagataa ccatacaggc gcgttcctac
     3361 atcgtgtgat tcgaataaac ctgcataaaa gacaggaaag agttgttgat caaaaatgtt
     3421 atagaaagac cacgtcccca caataaatat gacaaaaacc cagaagtttc gatccttgaa
     3481 aactgcgata aaatcctctt tttttacccc tcccgcatcc gccgctatgc actggtgatc
     3541 cttatcttta aaacgcatgt tgatcatcat aaatacagcg ccaaatagcg agaccaacca
     3601 gaagttgata tggggactga tactaaaaaa tataccggca aagaacgcgc caatagcata
     3661 gccaaaagat ccccaggcgc gcgctgttcc atattcgaaa tgaaaatttc gcgccatttt
     3721 ttcggtgaag ctgtcaagca aaccgcatcc cgccagatac cccaggccaa aaaagagcgc
     3781 ccccagaatt agacctacag aaaaattgct ttgcagtaac ggttcataaa cgtaaatcat
     3841 aaacggtccg gtcaagacca gaatgaaact catacaccag atgagcggtt tcttcagacc
     3901 gagtttatcc tgaacgatgc cgtagaacat cataaataga atgctggtaa actggttgac
     3961 cgaataaagt gtacctaatt ccgtccctgt taatcctaga tgtcctttca gccaaatagc
     4021 gtataacgac caccacagcg accaggaaat aaaaaagaga aatgagtaac tggatgcaaa
     4081 acgatagtac gcatttctga atggaatatt cagtgccata attacctgcc tgtcgttaaa
     4141 aaattcatgt cctatttaga gataagagcg gcctcgccgt ttacttctca ctttccagtt
     4201 cttgtcgaca tggcagcgct gtcattgccc ctttcgctgt tactgcaagc gctccgcaac
     4261 gttgagcgag atcgataatt cgtcgcattt ctctctcatc tgtagataac cccgtagagg
     4321 acagacctgt gagtaacccg gcaacgaacg catctcccgc cccagtgcta tcgacacaat
     4381 tcacagacat tccagcaaaa tggtgaactt gtcctcgata acagaccacc accccttctg
     4441 cacctttagt caccaacagc atggcgatct catactcttt tgccagggcg catatatccc
     4501 gatcgttctg tgtttttcca ctgataagtc gccattcttc ttccgagagc ttgacgacat
     4561 ccgccagttg tagcgcctgc cgcaaacaca agcggagcaa atgctcgtct tgccatagat
     4621 cttcacgaat attgggatcg aagctgacaa aacctccggc atgccggatc gccgtcatcg
     4681 cagtaaatgc gctggtacgc gaaggctcgg cagacaacgc aattgaacag agatgtaacc
     4741 attcggcatg tcgccagcag ggcaagtctg tcgtctctaa aaaaagatcg gcactggggc
     4801 ggaccataaa cgtaaatgaa cgttctcctt gatcgttcag atcgacaagc accgtggatg
     4861 tccggtgcca ttcatcttgc ttcagatacg tgatatcgac accctcagtt agcagcgttc
     4921 tttgcattaa cgcaccaaaa ggatcatcac cgacccgacc tataaaccca cttgttccgc
     4981 ctaatctggc gattcccacc gcaacgttag ctggcgcgcc gccaggacaa ggcagtagcc
     5041 gcccgtctga ttctggcaag agatctacga ccgcatcccc taaaacccat actttggctg
     5101 acattttttt cccttaaatt catctgactt acgcatagtg ataaacctct ttttcgcaaa
     5161 atcgtcatgg atttactaaa acatgcatat tcgatcacaa aacgtcatag ttaacgttaa
     5221 catttgtgat attcatcgca tttatgaaag taagggactt tatttttata aaagttaacg
     5281 ttaacaattc accaaatttg cttaaccagg atgataaaaa tgacgcaatc tcgattacat
     5341 gcggcgcaaa acgcactagc aaaacttcac gagcgccgag gtaacacttt ctatccccat
     5401 tttcacctcg cgcctcctgc cgggtggatg aacgatccaa acggcctgat ctggtttaac
     5461 gatcgttatc acgcgtttta tcaacatcac ccgatgagcg aacactgggg gccaatgcac
     5521 tggggacatg ccaccagcga cgatatgatc cactggcagc atgagcctat tgcgctagcg
     5581 ccaggagacg agaatgacaa agacgggtgt ttttcaggta gtgctgtcga tgacaatggt
     5641 gtcctctcac ttatctacac cggacacgtc tggctcgatg gtgcaggtaa tgacgatgca
     5701 attcgcgaag tacaatgtct ggctaccagt cgggatggta ttcatttcga gaaacagggt
     5761 gtgatcctca ctccaccaga aggcatcatg cacttccgcg atcctaaagt gtggcgtgaa
     5821 gccgacacat ggtggatggt tgtcggggcg aaagatccag gcaacacggg gcagatcctg
     5881 ctttatcgcg gcagttcatt gcgtgaatgg accttcgatc gcgtactggc ccacgctgat
     5941 gcgggtgaaa gctatatgtg ggaatgtccg gactttttca gccttggcga tcagcattat
     6001 ctgatgtttt ccccgcaggg aatgaatgcc gagggataca gttatcgaaa tcgctttcaa
     6061 agtggcgtaa tacccggaat gtggtcgcca ggacgacttt ttgcacaatc cgggcatttt
     6121 actgaacttg ataacgggca tgacttttat gcaccacaaa gctttgtagc gaaggatggt
     6181 cggcgtattg ttatcggctg gatggatatg tgggaatcgc caatgccctc aaaacgtgaa
     6241 ggctgggcag gctgcatgac gctggcgcgc gagctatcag agagcaatgg caaacttcta
     6301 caacgcccgg tacacgaagc tgagtcgtta cgccagcagc atcaatctat ctctccccgc
     6361 acaatcagca ataaatatgt tttgcaggaa aacgcgcaag cagttgagat tcagttgcag
     6421 tgggcgctga agaacagtga tgccgaacat tacggattac agctcggcac tggaatgcgg
     6481 ctgtatattg ataaccaatc tgagcgactt gttttgtggc ggtattaccc acacgagaat
     6541 ttagatggct accgtagtat tcccctcccg cagggtgaca tgctcgccct aaggatattt
     6601 atcgatacat catccgtgga agtatttatt aacgacgggg aggcggtgat gagtagccga
     6661 atatatccgc agccagaaga acgggaactg tcgctctatg cctcccacgg agtggctgtg
     6721 ctgcaacatg gagcactctg gcaactgggt taacataata tcaggtggaa caacggatca
     6781 acagcgggca agggatccgc gtcactcttc ccccttcacg accttcaata atatgcaatg
     6841 cagcttcccg cccgataatg tcatgtggaa gctgaattgt ggtcagcggc ggtaaaaaca
     6901 gatgcccgac gccaaccaga ttatcaaagc ccattacggc gacatccttc gggatacgta
     6961 cccccttcgc caaaagaacc tgataagcca caaaggctgc gcgatcgtta ccacatatca
     7021 gaacatcaaa atctggtttg cccgatttga agtggtcatt gagtaaactt gcgagatcgg
     7081 tgtagtgatc atcacctgtt gccatgtgaa attgtttcac ctcagccaga tctcgtccag
     7141 catcacgcca ggcctgctca aatccctgcc gacgataccc tgttgccaac gcactttccg
     7201 gtagccagaa gcataacggt tgacgatagc ccgccgcgag caaatgctgt gttgattcat
     7261 attgtgcagt gtaatcatca gggatataac tgggtaacgc tgggtcatcc gccacacagt
     7321 tcgccaatac aatattttca ccatacagag actcaggcag cgtgatatgt cgcagcccca
     7381 ttgtagtata gataatgcca tccggacggt gggcaagcag ctgacgtgcc gcgcgggcag
     7441 cgtcatcttc agaaaaaata ttgattaaaa aactattcca gccgaactcg ctggcggttt
     7501 gctcaatggc aagcagaata tcaacagaga aaggagtggt agcagtgtcc tgcgccagca
     7561 cggcgagagt cgacggctta cgtccttgag cgcgcatctt acgggcggaa agatcaggaa
     7621 cataattcag ggtctggatt gcctgcaata cgcgatcacg cgttgcagga cgcacagatt
     7681 ctgcattatg catcacccgg gagactgtca tcatcgacac tcccgccagg cgtgcgacat
     7741 cctttaatga agccataccc aagccgtttg ccgtaaaacg ggcactgtag cagaaacaga
     7801 cgtcactggc gagatccaac gatattctcg acgccctatc acccgacacg gcaatacaat
     7861 aaaaaataac aataattccc ggacaattgt acccaattcc gcctctgttc tcgccaacga
     7921 gtaaccatgt aggcaagtag gtaaaacatg ccaactggca tcgccaatct ggagaagaaa
     7981 atataaatat ggatcattgt gctgccgctg tatcccgaca tcagcccgga aattattgcg
     8041 attgctatcg gttcaggtgc gattggctgc acgatcgtta cggactcgct tttctggctg
     8101 gcgaagcaat attgcggcgc tacgctcaat gaaacattta aatactatac gacagcgaca
     8161 tttatcgctt cagtcgtcgc tctggcgggc acattcctgc tgtcatttat catctaagcg
     8221 caaagagacg tactatggaa aacgctaaaa tgaattcgct catcgcccag tatccattgg
     8281 taaaggatct ggttgctctt aaagaaacca cctggtttaa tcctggcacg acctcattgg
     8341 ctgaaggttt accttatgtt ggcctgaccg aacaggatgt tcaggacgcc catgcgcgct
     8401 tatcccgttt tgcaccctat ctggcaaaag catttcctga aactgctgct actgggggga
     8461 ttattgaatc agaactggtt gccattccgg ctatgcaaaa acggctggaa aaggaatatc
     8521 aacaaccgat cagcgggcaa ctgctactga aaaaagatag ccatttgccc atttccggct
     8581 ccataaaagc acgcggcggg atttatgaag tcctggcaca tgcagaaaaa ctggctctgg
     8641 aagcggggtt gctgacgctt gaagatgact acagcaaact gctttctccg gagtttaaac
     8701 agttctttag ccaatacagc attgctgtgg gctcaaccgg aaatctgggg ttatcaatcg
     8761 gcattatgag cgcccgcatt ggctttaagg tgacagtaca tatgtctgct gatgcccggg
     8821 catggaaaaa agcgaaactg cgcagccatg gcgttacggt cgtggaatat gagcaagatt
     8881 atggtgttgc agtcgaggaa ggacgtaacg cagcgcagtc tgacccgaac tgtttcttta
     8941 ttgatgacga gaattcccgc acgttgttcc ttgggtattc cgtcgcaggc cagcgtctta
     9001 aagcgcaatt tgccgagcaa ggtcgtatcg tcgatgctga taaccctctg tttgtctatc
     9061 tgccgtgtgg tgttggcggt ggtcctggtg gcgtcgcatt cgggcttaaa ctggcatttg
     9121 gcgatcatgt tcactgcttt tttgccgaac caacgcactc cccttgtatg ctgttaggcg
     9181 tccatacagg attacacgat cagatttctg ttcaggatat tggtatcgac aaccttaccg
     9241 cagcggatgg ccttgcagtt ggtcgcgcat caggctttgt cgggcgggca atggagcgtc
     9301 tactggatgg cttctatacc cttagcgatc aaaccatgta tgacatgctt ggctggctgg
     9361 cgcaggaaga aggcattcgt cttgaacctt cggcactggc gggtatggcc gggcctcagc
     9421 gcgtgtgcgc atcagtaagt taccaacaga tgcacggttt cagcgcagaa caactgcgta
     9481 atgccactca tctggtgtgg gcgacgggag gtggaatggt gccggaagaa gagatggagc
     9541 aatatctggc aaaaggccgc taattaattt tcaattcagc atcgccatcc tttccctggg
     9601 tgagcgatgc tgccgatggc gcagacttaa ggtccctggt cttacctgct ataaccccct
     9661 ccttttatca tcacccaacg cctttcgctg taaacggcgg tttcgcaaac caaaccaaaa
     9721 ctgtaagtaa gataaaagca atagccgcca tacggaaaat ctcatttgca gaaattgaaa
     9781 gtgactgctg agttatttca ttattaattt cattaagaac ccctgaaagc gaaccatagt
     9841 atttatccat aatttgtgac gatgaattaa acacggggtt aaattgatcg atggttgctg
     9901 tcaactgact atggtgtaac gattcgcgtc gtccccacag cgtcattgtc aacgacgtac
     9961 caactgatcc tgacaaggta cgaaaaaaat tactcatact cgaggcattg gcaaatttat
    10021 tatctggcaa acctgaaaac gaaatcgttg ttaagggtaa aaagaaacag gcgacggcga
    10081 atccctgaaa aaactgtggc aaaatgatgc ctgtaaaatc aatcgttggc ataaatgtca
    10141 cagaacgcca gtaatagcaa accgcataca ttaaaaaact aaatgtcacc aacaaccgca
    10201 tatcaatttt gttgccataa cgtcctatca aaggtgatat taatagcggc atgatgccga
    10261 tgggcgcata ggcaagtccg gcccatatcg cattataccc catcgtttcc tggagtaact
    10321 gcggcataag gactatcgcc ccagagtaaa ataaatatgc gcatgtgata ctcacaatac
    10381 caatggtgaa attacgggac ttaaacaaac tgagatcaag aatcgggttc tctgaggtcg
    10441 actcccaaat gactaaagaa atcagagaaa taactgacat tactgttagt attattattg
    10501 tactcgagtt gaaccaatcc agatcgcgcc ctttatcaag cattatctgc aagccaccaa
    10561 caccgagcac taacagggtc agtcctggta gattcatttt gaccggtgaa gtctcagttt
    10621 ctcttccttt aagtaaggtt aagcataatg tcagaacgat aatccccata gggacattga
    10681 ttaaaaatat ccaaccccag ctaaagttat cacaaatata accgcccaat atcggcccac
    10741 atatcggcgc gataatcacg gtcatagacc ataatgccag agcaaatgtt cttttttctg
    10801 gtggataatt ccttaataat aaactctgtg acagtggaat taacggcccc gccattaatc
    10861 cctgaacgac tctaaaaaat atcagcacat caagattggt cgataaacta cacattaagg
    10921 aagacagcga aaaaaaagtg actgaaagta aaaataatct taattcgcct attctttgtg
    10981 ccagcctgcc ggtaacaggg atcgcaatgg catttgctac gccaaacgaa gtgataaccc
    11041 aggtgccttc gtctgttgat gctcccagaa agccagatat tgtcggtatt gcgacgttag
    11101 aaatagtgga atccaacatt tgcataaacg tcgctaatga caatgcaata gtgacgcacc
    11161 ataacgtccc accggttaat ggtgccggag ttgatttagt gattgccatt aaagttgtcc
    11221 attatgcgaa ataatgttgc taatttcttt ttctatcgga ctggtatcga taactaaagc
    11281 cttactggta taagcaggca tggacgtcac ggttgaagcc agctcaggca tctcggcaat
    11341 gtcttcgttc ttcgtatcaa tagttgctgt catcgataaa ccaatacgta agggatgttc
    11401 catgagttct tttggatcaa gagaaacttc aaccggtaca cgctgaacga ttttgatcca
    11461 gttccctgtc gcattttgtg caggtaataa ggagaacgca ttgccggttc ccatattgat
    11521 ccctgtcacc cgaccatgaa acacaacatt ttcaccataa agatcgctga taatattgac
    11581 cgattgacca atccgtacat ccgtgagttg tgtttcttta aagttagcat taacccacat
    11641 ttgacgtgcc ggtacgacag ccattaacga ttgtccggga ctcactgttt cgccgacctg
    11701 aacacttcgc tgggcaatgt agccggtaac cggactctta atatccgtac gtttaagcgc
    11761 caaccaggct tctttggttg catccgcagc ttcaacgact tgtggctgac ggtttaatgg
    11821 tgtgttcatt actaaagctt tattcgcttt ataagcctga atagcagcat tcaatgccgc
    11881 tttgctactt attaacgtat ctttggtatg ctccagcgtt tcttttgaaa taaccccctg
    11941 cttcgctaaa ggtacacgac ggttataatc ttctaaagat tgttgatact gaatacgtgc
    12001 tgaagcgact tcggcactgt attgtttatc ctgtaagtac agtttattcg tttgccgaac
    12061 aatatttgcc agattatttt tagctttatt gagtgcgata gtggcatcag tcttatccag
    12121 tgagactaaa atgtcaccct gtcgaacgta gttcgtatct ttatgattaa cgacagtgac
    12181 gctgcctgaa acttgtgcag aaattggatc tgcattcccc gtgacatagg catcatctgt
    12241 actaatcatg tcttctaatt ccattgacca ataggcatag gcacctgaaa acgcaataaa
    12301 taaaactacc gccaataaag aaaagtattt tcttctgtta gaatgttttt tatttgaatt
    12361 aatctgttcc actattatct ctcatttcgc atagatgaaa tttatgaatt gaatgaaaag
    12421 agtattcatt cttcatatca aacacagtgc atgtgttgca cattgtccca ggaacgttgg
    12481 aggcctaatt taaatgaggt gcaaaaaaaa caacagagga ctaaaccgtg gcttttgcaa
    12541 tacaattctt acgcatgtag gattagtgag aagacttata gtgctaatgt gaaactataa
    12601 atcatcggta caatccctga ttttattgtt gacatttcat ttatgccgac tatttatatg
    12661 gtatacttgt cgaattatct taaaggaagc tctaattttc ttatttttat tgagaaaatg
    12721 agatgatgcc ttatgtctgt attactacag ggagaaggga gatgcttcat tgcaaaggga
    12781 ataatctatg aacgcaataa ttattgatga ccatcctctt gctatcgcag caattcgtaa
    12841 tttattgatc aaaaacgata ttgaaatctt agcggagttg actgaagggg gaagtgccgt
    12901 tcagcgggtg gaaacactta aacctgatat cgtcatcatt gatgtcgata tccccggagt
    12961 taacggtatc caggtgttag aaacgctgag gaagcgccaa tatagcggaa ttattattat
    13021 cgtctccgct aaaaatgacc atttttacgg gaaacattgt gctgatgctg gcgctaatgg
    13081 attcgtgagt aaaaaagaag gcatgaacaa tatcattgcg gctattgaag ctgcaaaaaa
    13141 tggctactgc tatttcccct tctctctcaa ccggtttgtt ggaagtttaa cgtccgacca
    13201 gcaaaaactc gactccttat cgaaacaaga aattagtgtc atgcgatata ttcttgatgg
    13261 caaggataat aatgacattg cggaaaaaat gttcatcagc aacaaaactg tcagcactta
    13321 taaaagtcgc ctgatggaaa aattagaatg taaatcactg atggatcttt acacattcgc
    13381 acaacgtaac aaaatcggct aaccacatga agtttttacc ctatattatt cttctctgtt
    13441 gtggtctttg gtcgaccata agtttcgcag acgaagatta catcgaatat cgtggcatca
    13501 gtagtaacaa ccgtgtcacg cttgatccac tacgtctgag caacaaggaa ttacgttggt
    13561 tagcgagcaa aaaaaatctt gtgattgcag tacacaagtc ccaaacggct actttgttgc
    13621 ataccgattc gcagcaacgg gttcgtggta ttaatgctga ttatttaaat cttttaaaaa
    13681 gagcgttaaa tatcaaatta acactccggg aatacgcaga tcatcaaaaa gcaatggacg
    13741 cgcttacaga aggggaagtc gatatagtgt tatcacattt agttgcttcg ccgcctctta
    13801 atgatgacat tgctgcaacc aaaccgctga taattacctt tccggcgctg gtcaccaccc
    13861 ttcacgattc aatgagaccg cttacctcat caaaaccagt aaatattgct cgggtagcaa
    13921 attaccctcc agatgaggta attcatcaat catttccaaa agcaacaatt atctctttta
    13981 caaatttata tcaggcatta gcatccgtct cagccggaca gaatgattac tttattggta
    14041 gtaacattat taccagcagt atgatttccc gctatttcac tcactcctta aatgtagtga
    14101 aatattataa ctcgccgcgt caatataact ttttcttaac cagaaaagat tcagtcgttc
    14161 ttaatgaagt actcaatcgg tttgttgatg ctttaacaaa tgaagttcgc tatgaagtat
    14221 cacaaaattg gcttgataca ggaaacctgg cctttctgaa caaaccatta gaactcactg
    14281 aacatgaaaa acagtggatt aagcagcatc ccgatttaaa ggtgctggaa aatccttact
    14341 cgccacccta ttctatgaca gatgaaactg gctcagttcg gggcgtgatg ggtgatattc
    14401 ttaatattat taccttgcaa acaggtttaa atttttctcc gatcaccgtt tcacacaata
    14461 ttcatgcggg gacacagctt aaccccggtg gatgggatat attgcccgcc gctatttata
    14521 gtgaagatag agaaaataat gttttatttg ctgaagcctt cataacaacg ccttacgttt
    14581 ttgtcatgca aaaagcgcct gacagtgaac aaacattaaa aaaaggaatg aaagttgcca
    14641 ttccctatta ttatgagctt cattcgcaat taaaagagat gtatccggag gtagagtgga
    14701 taaaagtcga taacgccagc gctgcatttc acaaggtcaa ggaaggcgaa cttgatgctc
    14761 tggtcgcgac acagttaaat tcacgttaca tgatcgacca ttactatcct aatgaacttt
    14821 atcattttct tattcccggc gttccgaatg catcgctttc gttcgctttt cctcgcggag
    14881 aaccggaact taaggatatt attaataaag cactgaatgc aattccccca agcgaagttc
    14941 tgcgcctgac ggaaaaatgg attaaaatgc ccaatgtgac cattgacaca tgggacctct
    15001 atagcgagca attttatatt gtcacgacat tatccgtttt attagttggc agtagccttt
    15061 tatggggatt ctacctgtta cgctcagttc gtcgtcgtaa agtcattcag ggtgatttag
    15121 aaaaccaaat atcattccga aaagcactct cggattcctt accgaatcca acttatgttg
    15181 taaactggca aggtaatgtc attagtcata atagtgcttt tgaacattat ttcactgcgg
    15241 attactacaa aaatgcaatg ttaccattag aaaacagtga ctcacccttt aaagatgttt
    15301 tttctaatgc gcatgaagtc acagcagaaa cgaaagaaaa tcgaacaata tacacacagg
    15361 tatttgaaat tgataatggc atcgagaaaa gatgcattaa ccactggcat acattatgca
    15421 atctgccagc cagcgatcat gctgtttata tttgtggttg gcaagatatt accgagacgc
    15481 gtgatttaat tcatgcactc gaggtagaga gaaataaagc gatcaatgca actgtcgcaa
    15541 aaagccagtt tctggcaaca atgagtcacg aaataagaac accaataagc tccattatgg
    15601 gcttcctgga acttctgtcg ggttctggtc ttagcaagga gcaacgggtg gaggcgattt
    15661 cacttgccta cgccaccgga caatcactcc tcggcttaat tggtgaaatc cttgatgtcg
    15721 acaaaattga atcgggtaac tatcaacttc aaccacaatg ggtcgatatc cctactttag
    15781 tccagaacac ttgtcattct ttcggtgcga ttgctgcaag caaatccatc gcattaagtt
    15841 gcagcagtac gtttccagaa cattaccagg ttaagatcga ccctcaggcg tttaagcagg
    15901 tcttatctaa tttactgagc aatgctctta aatttactac cgagggggca gtaaaaatta
    15961 cgacctccct gggtgacatt gatgacaacc acgctgttat caaaatgacg attatggatt
    16021 ctggaagtgg attatcgcag gaagaacaac aacaactgtt taaacgctat agccaaacaa
    16081 gtgcaggtcg tcagcaaaca ggttctggtt taggcttaat gatctgcaaa gaattaatta
    16141 aaaacatgca gggcgatttg tcattagaaa gtcatccagg cataggaacg acatttacga
    16201 tcacaatccc ggtagaaatt acccaacaag tggcggctgt cgaggcaaaa gcagaacaac
    16261 ccatgacact ccctgaaaag ttgagcatat taatcgcgga tgatcatccg accaacaggc
    16321 tattactcaa acgccagcta aacctattag gatatgatgt tgatgaagca actgatggtg
    16381 tgcaagcgct acacaaagtc agtatgcaac attatgatct gcttattact gacgttaata
    16441 tgccgaatat ggatggtttt gagttgactc gcaaactccg tgagcaaaat tcttccttac
    16501 ccatctgggg gcttacagcc aacgcacagg ctaacgaacg tgaaaaaggg ttaaattgcg
    16561 gcatgaactt atgtttgttc aaaccgttga ccctggatgt actgaaaaca catttaagtc
    16621 agttacacca ggttgcgcct attgcacctc agtatcgcca ccttgatatc gaggccctga
    16681 aaaataatac ggcgaacgat ctacaactga tgcaggagat tctcatgact ttccagcatg
    16741 aaacgcataa agatctaccc gctgcgtttc atgcactaga agctggcgac aacagaactt
    16801 tccatcagtg tattcatcgc atccacggtg cggctaacat cctcaatttg caaaagttga
    16861 ttaatattag ccatcagtta gaaataacac ctgtttcaga tgacagtaag cctgaaattc
    16921 ttaagttgct gaactctgtt aaagaacaca ttgcagagct ggaccaggag atagctgttt
    16981 tctgtcaaca aaatgactaa atagcggctc ccacaatatt caaatgtggg agctatttac
    17041 cagcacatct tacatttatg atgaaaactc ctggcgaatt tgttccccat gttggtcgag
    17101 cgttgccgct cccggcataa catgcgggtc cgcgcagccg ctgattttta tcggattacc
    17161 tggcatcatt attcccccgg cttcaatcaa catatttctc gcctgagttt gtggcaaatt
    17221 aatggcctca gccacactta ataacggcgc gacgggcaca ccaacttcat gtattttggc
    17281 taaccaaact tcagctgcct gcgtttttaa cgttcgctca atatattgtt taagaatagc
    17341 ctggttttgt acgcgtaaaa tattgctgct aaatcgggga tcattaacca gttccgtaag
    17401 ctccagtgcc tggcataacg cagaaaaaag cttgtcatta ccacaacaaa tcgtaatcgg
    17461 cttatcctga gtatcaaaaa catcaaaagg tgccatgtag ggatggcgat ttcccaggcg
    17521 ttgtggtgac ttccctgtcg caatatatgc catcagacca tgctccagaa aactcagcgt
    17581 ggcatcaaac atcgctatat cgacatgcgc ccctctctgg ctcttttcgc ggccataaag
    17641 tgcactcact attccgctga ataaatagac accgccacat aaatccgcaa gagaggtacc
    17701 cacgcgcact ggcggagcat cagggtaccc tgtttccatc attatcccgc tcattgcctg
    17761 aatgatggta tcgtaggctg gagcatcttt tagcgggccg gtatgcccaa aacctgacga
    17821 tgaagcatat atgagacgcg ggttgatttc ttgtagcgtt tcccatgaaa accccagttt
    17881 ttccattgta cctgggcgaa aattctcagc taatacatca gcttgtttaa gcatatttat
    17941 aaatatactt ttatcgtgat cattctttaa atcaagaacc acactctctt tgccatgatt
    18001 aataaaactg taatagagtg actgtccatc cacataggga ccaaatgtgc gggtatcatc
    18061 accatgaccc ggcggctcaa ctttaattac ccttgcgccc atattacaaa gaagttgagt
    18121 tccgaaaggt ccattaagga catgtgtcat atcgataact aataagcctt caaacggccc
    18181 tttgcattca ttatttgtca tttgcttatg ccttgaaaaa agccgaaggt gaaaagacac
    18241 ttcggcattt atgtaaaatc aataacatga ttaattatga aaccagtcga ctgacataaa
    18301 tccacaaagg agccgtgacg acaaagccca gtacgctcac agccagtgac gctgtaccag
    18361 tgcgggtgta gacattaaac cggctggcaa taatgattcc ggagaatgcc ggcggtaagg
    18421 cacctgccag taccatcatc tgcaaatgtt cgctgttcaa atgacatgcc atacccacga
    18481 gaagcagcgc cagtggcatc agaatcagct tcaggaaggt gttataagca atttcggcac
    18541 tgaattcgaa tttatgtgca gccagagtca accccgcagc gaataccgct acccctgagt
    18601 tagccttcgc aatcagatta aaggttgggt cccatgctgc cggaattttt accccaacca
    18661 acaccaggat cgttgccaga acaggtgccc atactactgg ctcctttgcc gcagaaatta
    18721 atgcgctcag attactattc ttcttaccat ccgctcctga ggaaggattc agcaaataca
    18781 gaccaatagg aatagtaatt gcgttaacaa taatagaaat aattgctaca actaaacctg
    18841 ttgatacgga atcgccataa ataggatcga gaactgcaaa tcccaggaat ccgatggtag
    18901 gtgaacctgc aattaatgca catactgctg cttctgcatg agtacgttta aaaaatttgt
    18961 agcaaccaaa ccaggagaaa aagaaacatc cgacaataac cacaagtgat acaagggtca
    19021 ggcgagtgtc cgcaaaaatc atttccctgt ttgcccgggt aatagaaaca aatagggccg
    19081 caggaagagc gtagtttaat accagtttat taaatgcccg ggcttgatct tctgaaaaag
    19141 tttctcgtct gccgctaaaa taacccaaca gcatgattac gataataggc aataaatcgc
    19201 caataaaaaa tgttagcata atagtatttc cgataagggt cccctcacca tcgataatga
    19261 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    19321 ggtaatatgg ccactttcag ttcctgctgc agggtcgatg accacattaa taatggtcgg
    19381 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tagtgacgtt
    19441 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaatagatc
    19501 tgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    19561 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatctccat
    19621 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaggtcac
    19681 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    19741 acggcgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    19801 ttcattaact aaataaatat cctggttctc gcgcaataca tcgcgcacag cacttaatgc
    19861 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    19921 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    19981 tttcaattct gccagcatac cttgcatact ggatgcaata tcgccaacga ctggcacagc
    20041 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    20101 tgccgcccat ccttttttac cgtgtgccag taaccaattc agtcgtgcac cgacaagcat
    20161 gacaacgtca gcatttgcca gggcaaacga acgcgcagtt gccgcagaaa gtggatgtgt
    20221 atcttcgagg atccctttcg ccatagacat tggcaggaat ggaatctggg cactttcaat
    20281 aaattcacga agctgttcat ccgcttgtga atacgccgcg cctttgccaa ggataactaa
    20341 tggtcgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    20401 taatgctggc gacggatttt caacttttac aatcgtggtt aacgcttcgt ctttttccat
    20461 cgtcgcggcc aggacatttg ctggtaaatc aagataaact ccgccagggc gacccgatac
    20521 tgagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    20581 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    20641 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgatcatcg gaaaaccatt
    20701 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag acactgtcag
    20761 gcagatccct ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    20821 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgcg ccatatccgt
    20881 cacaggaata cctacaacac cataaatagt gtcaatatca ttctgtttta atgcttcaac
    20941 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    21001 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    21061 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    21121 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaact
    21181 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    21241 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    21301 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    21361 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    21421 caatctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    21481 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    21541 ccgccaccac ccgcattacc tccgcgggga actgcatcac caaatgtgcc attcggatac
    21601 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcgcg taatttcacg
    21661 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    21721 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagta aatgcattcc ggtgttgctg
    21781 tcacccaacg ctgcagcgct taccagcggc ggaccgtccc aaaaacccgt agtggatgcc
    21841 gcaccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    21901 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    21961 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    22021 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taattcaata
    22081 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    22141 agctggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaatgac gtcagcgcca
    22201 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    22261 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatatgttaa
    22321 tttagcgttt atttaaggca taacgccctc tataaataat ccgcaaaata tcgtcgtagc
    22381 tgcactcgat atttttaaat tcctgtatca agtgctgaaa atcatttcat cgccacttcc
    22441 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattggttc
    22501 gcggtaatca gagagagccg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    22561 ggtgatttac aagtaagggt atgattacgc cttttaaagc gtaccataaa acataacata
    22621 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaga taaaatgcga
    22681 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    22741 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcgtt
    22801 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    22861 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    22921 gagtctgttt gagcggcatc agatattggt tttccattac gccaacaccg gccaggcgca
    22981 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcgttggca atttttatcg
    23041 cggcttcttt ttcgggtgtc gctacataac tctcagaaat tccgacagat gcattaatga
    23101 caatatattg gtcatcattg acattggtct ttttacctgg cttagcgaac tttgcccatt
    23161 cggtactgtc atctgacaac aaagctgaag cttcattggt cagttctttt gccttttccg
    23221 gatcgccatg aaacagagcc aagcgcgcta cctgcacgtc acgcatcgca tataaacctt
    23281 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgaacttgc tgagtttgct
    23341 gttgtgccgc taccggtgca ttatcagccg cccatacagt tgaagatgcc agaattgctg
    23401 tgaccatcgt agccataatt aaacgtttca tacttacctc attctttaac agttttagaa
    23461 aacgccgcca tcatattggg tgagcatgaa atgagaaatc agagagcaac ggtcattttg
    23521 aaagggttat cttacagttg tagttattat cgcaagaagt attttagtct tacaattgta
    23581 gga
//
LOCUS       DACBVI010000063        23031 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.063, whole genome shotgun sequence.
ACCESSION   DACBVI010000063 DACBVI010000000
VERSION     DACBVI010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.063"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            137..1159
                     /locus_tag="IU325_12010"
     CDS             137..1159
                     /locus_tag="IU325_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP5809746.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQFPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFATSPAANLSFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDVPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCPLSDADY
                     QKLVAS"
     gene            1159..2139
                     /locus_tag="IU325_12015"
     CDS             1159..2139
                     /locus_tag="IU325_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP5809747.1"
                     /translation="MRTVNGCILLLAAISITFAAVSGAYHLDIQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALFLGAALAGAGTTYQGMFRNPLVSPDILGVSAGAGLGACAA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLFLLLRGGRQ"
     gene            2136..2894
                     /locus_tag="IU325_12020"
     CDS             2136..2894
                     /locus_tag="IU325_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP5809748.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAIKH"
     gene            2904..3716
                     /locus_tag="IU325_12025"
     CDS             2904..3716
                     /locus_tag="IU325_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP5809749.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLTAKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLKVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAMASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVQPEALR"
     gene            3713..4567
                     /locus_tag="IU325_12030"
     CDS             3713..4567
                     /locus_tag="IU325_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HAP5809750.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFHRQGG
                     CVSGISVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYATPADIKV
                     SLQPAASI"
     gene            4593..6563
                     /locus_tag="IU325_12035"
     CDS             4593..6563
                     /locus_tag="IU325_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAP5809751.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEANLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTQYAYSEGFIEEGRNFWAGIEYTF"
     gene            complement(6613..6867)
                     /locus_tag="IU325_12040"
     CDS             complement(6613..6867)
                     /locus_tag="IU325_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP5809752.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            7068..7802
                     /locus_tag="IU325_12045"
     CDS             7068..7802
                     /locus_tag="IU325_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HAP5809753.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRISWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNNAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AGLHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(7804..8415)
                     /gene="emtA"
                     /locus_tag="IU325_12050"
     CDS             complement(7804..8415)
                     /gene="emtA"
                     /locus_tag="IU325_12050"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HAP5809754.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            8515..9429
                     /gene="ldcA"
                     /locus_tag="IU325_12055"
     CDS             8515..9429
                     /gene="ldcA"
                     /locus_tag="IU325_12055"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HAP5809755.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVDVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPQIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEPRTVT
                     LPLGAHAILTNTREGTQLTISGHPVLKM"
     gene            9524..11260
                     /locus_tag="IU325_12060"
     CDS             9524..11260
                     /locus_tag="IU325_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HAP5809756.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11362..11649)
                     /locus_tag="IU325_12065"
     CDS             complement(11362..11649)
                     /locus_tag="IU325_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809757.1"
                     /translation="MHPVLQADETPLQLLSSLKRVLCGLRQCGRLRGGICSGDVRLGP
                     LMVDGDTGNAGLVILHLLQSEEAIMLTFLSRRFELISQSLQWFVIHNLTFF"
     gene            11910..13346
                     /locus_tag="IU325_12070"
     CDS             11910..13346
                     /locus_tag="IU325_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809758.1"
                     /translation="MASIKDIRPFAPGIPPSSVNPGVRPPGQFFLPVELKNEILRNMK
                     YIGPQIFSWGDDHRQIIDLSTAGEVKIHMVDNTIITLERLNPNPQHNEFMMVAEWCQW
                     AMERDGLQENLQKNLYEILEENQQNKQSEIPQEDLKESLEEIKENILEENLPASRIEN
                     RAEALRRMKECLITRQSMLNLSNLGLTSLPENLPPHLIEFYCSKNVLTALPKVMPKWL
                     LVLDCTDNVLILLPKVQPSKLMVLKCYENCIIWLPELSTNLRVINCSENFLQFLPPSM
                     PQYLYKLSCAGNNIHSIPDEMLENLTRLKVFDCSSNDLISAPRLPPKLIIYYCGENKF
                     KTVQVPQPQSLKVFDCNGNPWDKDNLPTLFKAVEGLKKQQGLKDLLGFLHKEGQMKLE
                     KLEKLEDLDDLMDLELLDDPVLLERVKVQEDLELLNQQLGLLILERQQDSQPVNQHSE
                     HEPESASRVKRDLSEVEFESTMKRKRFM"
     gene            complement(13514..14584)
                     /gene="dadX"
                     /locus_tag="IU325_12075"
     CDS             complement(13514..14584)
                     /gene="dadX"
                     /locus_tag="IU325_12075"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HAP5809759.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMERIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVMTV"
     gene            complement(14594..15892)
                     /locus_tag="IU325_12080"
     CDS             complement(14594..15892)
                     /locus_tag="IU325_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP5809760.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            16222..17754
                     /locus_tag="IU325_12085"
     CDS             16222..17754
                     /locus_tag="IU325_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HAP5809761.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            complement(17804..18523)
                     /gene="fadR"
                     /locus_tag="IU325_12090"
     CDS             complement(17804..18523)
                     /gene="fadR"
                     /locus_tag="IU325_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HAP5809762.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            18745..20286
                     /gene="nhaB"
                     /locus_tag="IU325_12095"
     CDS             18745..20286
                     /gene="nhaB"
                     /locus_tag="IU325_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HAP5809763.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            20432..20962
                     /gene="dsbB"
                     /locus_tag="IU325_12100"
     CDS             20432..20962
                     /gene="dsbB"
                     /locus_tag="IU325_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HAP5809764.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLLVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(21008..22276)
                     /gene="umuC"
                     /locus_tag="IU325_12105"
     CDS             complement(21008..22276)
                     /gene="umuC"
                     /locus_tag="IU325_12105"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
                     /protein_id="HAP5809765.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLAGTDIRFIRKHF
                     NVVLERTVRELHGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPPPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(22276..22695)
                     /gene="umuD"
                     /locus_tag="IU325_12110"
     CDS             complement(22276..22695)
                     /gene="umuD"
                     /locus_tag="IU325_12110"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAP5809766.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT         5528 a         6018 c         5782 g         5703 t
ORIGIN      
        1 attttcttgt ggaacaaagt gttatttaag aaacaagcaa atcattctgt ctctttagac
       61 aaattttgac atcaaacatg atctttttcg ctatatgtgt aagtatatag cggttttaat
      121 tatcaggata ttacttatgt cactaaccag acggaggttt acacagattc ttgcgtcgac
      181 gttgttcctg catcatctgc cgtcctttgc acaatcagtc aaattctggg cctcactgac
      241 gcttcccgaa gcccaaaaca ttacacgcat cgtcagcgca ggcgcgcccg ccgatttact
      301 attgctggct gtcgcgccag aaaaaatggt cggtttttcc tcttttgatt ttgcccgtca
      361 ggcattaatt ccattgccag agcacattcg ccagttcccc aggctgggac gactcgccgg
      421 gcgcgccagc acactctcgc tggaagggct tatggcgtta catcccgatt tggtcgttga
      481 ttgcggcaat acggatgaaa cctggatctc ccaggcacgg caggttagcg aacagacaca
      541 aataccctgg ttattgctta acgggaaact ggaacaatca gcagaacagt taacaacgct
      601 tggcaaaacg ttaggcgaag agcaccgcgc cgcagaacaa gccaatctcg ccagccgctt
      661 cgttggtgaa gctcaggcat tcgccacctc acccgccgct aacctcagct tttatgctgc
      721 gcgcggtcct cgagggctgg aaacgggctt acagggttcg ttgcataccg aggcggcgga
      781 attattaggt ttgcataacg tcgcgcaaat agccgatcgc cacggtctga cacaggtttc
      841 catggaaaat ctcctgcgct ggcagccgga tattattctg gttcaggagg ccgttactgc
      901 agattttatt cgtcgtgatc ctctctggca gggcgtgaaa gcggttgcgg aacaacgcat
      961 cttattttta agtggcctgc cctttggctg gctggatgtc ccgccgggaa tcaaccgtct
     1021 tctgggatta cgcagacttc acgcctggct ggatcccgcc atcaatcgcc agtttaaaag
     1081 tgacatgcag cattacgccc aactgttctg gcattgccca ttaagtgacg ccgactatca
     1141 aaaattggtg gcgagctaat gagaaccgta aacggatgta ttttgctgct ggcagctatc
     1201 agtatcactt ttgccgccgt aagtggggct tatcatctcg atatacagca actgctggcg
     1261 ctcattctgc gtcaagaaaa tgttcctgtg caggaacaaa ttgtcttctg gcaaatccgc
     1321 gtgccgcgta ttcttgctgc gctgtttctg ggcgcggcgc tggcaggggc cggaaccacc
     1381 tatcagggaa tgttccgtaa tcccttagtg tcaccggata ttttaggcgt ctccgccggt
     1441 gccggacttg gtgcctgcgc ggcaatcctg tggggattat ccattgttta catccagctg
     1501 tacgcatttt gcggcgggct gatggtggtc gcgggcgtct ggttgattac gcgtcgggtg
     1561 acccgccacg atccgatctt aaccctggtg ctggtaggca ttgcgttagg aacgttgtgc
     1621 ggagcgggga tatcgctaat aaaaacgctg gccgatcctt acacccagtt accgtcaatt
     1681 accttctggt tgctgggggg gctgtccacc gtgaccttac gcgatctctg ctatgctgca
     1741 ccgatcattc tcataggctc cttgcccctg ttttttctcc gctggcgcat gaatttactc
     1801 acgctttccg acgacgaagc ccgttcgctg ggactgaacg tgacacgtct gcgctttgga
     1861 ttaattgtct gcgccacact aatcaccgcc agcacggtag caatagcggg cataattggt
     1921 tgggtcggcc tggtcgttcc gcacattgcc cgcttgttga ccggtcataa ccatcagcaa
     1981 ctgttgccca tggcaatgtg tactggcgcg attctgctcc tgcttaccga tacgctggca
     2041 cgcagcattg gaacaacaga aatcccactt ggcattctga ccgcttttgt tggtgcaccg
     2101 tttttcctgt ttttgttgtt acgcggaggt cgccagtgac tttgcttgcc gtccgtcatg
     2161 cgtctctggg ttactcccgc catccggtgc tacgcgacgt gtccttcacc ctgtcgcaag
     2221 gaacaatttg ttgtctgtta ggagccaatg gttgcggcaa aaccacactc atgcgctcca
     2281 ttcttggcgt tataccgtta ttaaaaggcg agatcttact ggatagcctc cctgtgcaga
     2341 cactcagcca tcgccagcgc gcgcaggcta ttgcctgggt gccgcaggcg cacgacggca
     2401 tttttgcttt tagtgtgctg gatatggtgt tgatgggcct ggcccccacc ataggcgctt
     2461 tttccgttcc cggtaagcaa gaacgcctga aagcaacgga gcaactggaa aagctgggca
     2521 ttttgcatct ggcgacacga cgctggaata cgcttagcgg cggtgaacgt cagctggctt
     2581 taatcgcccg tgcactcgtc cagcaaccgc gcttgttatt actggatgag cctgcctcca
     2641 gcctcgattt cggtcatcag atccaactac tggacacgct ggcgcagctc aaaaataacg
     2701 gcatgacaat gttaatgtcg actcatcatc cgctccatgc caacgccatt gccgacagca
     2761 ttatccaggt agagccagat ggacgagtaa cacaaggctt accgaccgaa cagttaacaa
     2821 cgaacaaact ggctgccctg taccgggttt ctgccgacca gattcaccat cacctctctg
     2881 caattaagca ctaaggaaaa atcatgctca ttgacgatat tgattttgca gacctttacc
     2941 ttcagcaact caaactggcc caccgcacag aaaaaacgcc ggatcactgg gatcaacggg
     3001 cggaaaaaat ggcggaaaat tgcgcctcgc ctacggacag ctacctgcaa caactgaccg
     3061 ctaaaattga tctgcaaggc gcgcagacct tattcgatat gggctgcgga ccgggtaccg
     3121 tatcgctggc gctggctgac aaactcacga cgatttatgg cgttgattac agtcagggga
     3181 tgctgaaggt tgccgcccgt cgcgccgccg cgctaaaggc agacaatgtc cactggatcc
     3241 agcgcgcctg ggaagaggac tggagtgacc tgccacgctg cgatattgcc atggcttccc
     3301 gctccactct ggtggctgat atgcgccagg caatgagcaa actgaataat caggcacggc
     3361 ttcgcgtcta tacgactcat ctggtcagca cctcgttcgt ctctccagcc attcagcggg
     3421 ctgctggtcg ggaagtgatc gaattaccca actatatctt tgcgctcaac gtcctgtatc
     3481 agatgggaat ttatgctcac gttgatttta tccgtggaca aaattgccaa caggataaca
     3541 gcacctggga acgttttgag cagaacgtca gctggagtct gggcgcgctt aacgacgatg
     3601 agcgtgagcg gttatatcgc tggtatcaac aacaagatgc tcgtgccctc gcccctgcca
     3661 gccgcgactg ggcgctcatc tggtgggata gcgtgcagcc ggaggcactg cgatgatttt
     3721 cctttctcag gcacaaatcg atgcgttact tctggaagat atccagggcg gtgacctgac
     3781 cacgcgagca ttaaatattg gacaccagca tggctatata gagttttttc accgtcaggg
     3841 cggctgcgtc agcggaattt ctgtcgcgtg taagatgtta accacgctgg ggttaaccat
     3901 tgatgacgcg gtcagcgacg gttcacaagc gaacgcgggt cagcggctaa tccgtgcgca
     3961 gggtaatgcc gcagcacttc atcagggatg gaaggcagtc cagaatgtgc tggagtggag
     4021 ttgcggtgtt tctgattatc tcgctcaaat gctggcgtta cttcgtgaac gttaccctga
     4081 tggcaatatc gcctgcaccc gaaaagcgat tccgggcacc cgcttactgg cctcgcaggc
     4141 aattctggct gccggaggac tgattcatcg tgccggatgt gcggaaacca tattattgtt
     4201 tgccaaccac cgtcattttc ttcatgacaa tcaggactgg tcaggcgcaa tcaatcagtt
     4261 acgtcgccac gcacccgaga agaaaatagt tgttgaagcc gacacgccga aagaggcaat
     4321 cgccgcgtta cgcgcgcaac cagacgtact tcagctcgac aaatttagtc cacaacaggc
     4381 aactgaaatt gcgcaaatag caccgtcgct ggctccccac tgcacgctgg ctctgaccgg
     4441 cggaattaat ctgacaacac tcaaaaatta cctcgactgc ggcattcgcc tttttattac
     4501 ctccgcgcct tattacgcga caccggctga cattaaagtc agtctgcaac ccgcagcctc
     4561 tatttaattc tttgttaaac ggaatttatt ctatgagact caaaaaacgt tatctctgca
     4621 cagcattatc acttgccttt acccagcagg ccgtagcggc tcaggagagt gacacgctga
     4681 ccgtatggtc cagtccggta tcatcgacga cgaccaccgt tctcgatcaa cccaccatga
     4741 aggccctgga taaacagaat gtcgctcagg cattaagtgt cgtccccggc gtggtgctgc
     4801 aaaagtcagg tagccgcaac gaagaacagg tcaaagttcg tggctttgat agtcgtcagg
     4861 tgccggtcta tttcgacggt gtgcccattt atgttcccta tgacggcaac ctcgatctgg
     4921 cgcggattct gaccaacaat ttgggggcag ttgaagtttc caaagggtat tcgtcgctgc
     4981 ttcaggggcc taatcagatg ggcggagcca ttaatatcac cacccagaag ccaacaaaac
     5041 ctctggaagc aaatctggga tatcgccagg gatggagccg tagccaggac aatgcctacg
     5101 atatgcatgc ttcatttgcc gccagcagcg atctggggta tttgcaagtc agcggtagcc
     5161 agctaaagca ggattttctc ggcctgccgc atggtgtaaa taatgatatt gcaggcaaac
     5221 acggcaagat gattaattca tcggctgatg ataaacgcgg cattgtgaag ctcggtttta
     5281 caccacgcga aaacgatgaa tacacattga cttacattaa gcaggatggt gaaaaagata
     5341 acccgccata cagtggaaat agtggtcaaa aatcacgcta ctggcagtgg ccagagtatg
     5401 acaaagaaag tttttattat cagggaacga cccaactaaa cgatcgtttt accctgaaaa
     5461 gtcggctgta tcgcgacacc tttgaaaata cgctgatgat gtacaactcg ctggctgatt
     5521 tgaaaaataa aaaaggcagc tacagccatt attccgatta cagcgacggt gccgggttac
     5581 aactggcagc cgatgtgcgt gaaaacgatc tgctgtcgtt tgccgttaac tggaaagatg
     5641 acgtacaccg ggaaaaaggt gcgccgcacg ccgcttacga tcgctatgaa gatcgtacct
     5701 ggtcgctcgc cagtgaatat caatgggctg ctgccgataa tgtcgatgtc gtggctggaa
     5761 tcagctatga ctggcgcgat agcgtagaag cgaaaaaaca tgagaaagat ggaagcatca
     5821 cccattatga cgacaacaat cagtcagctt ttaactggca ggtgatgggg aaataccact
     5881 ttgccaatga agacacgctg gcgctttcgt actatgaccg cacacgcttt ccgacgctga
     5941 aagaacgcta taccacgtcc aaacctgcgt ataaccagat agcgattgtt aacccgcagc
     6001 tcaaaccgga acgcgcgcgc ggggtggatt taacctggaa tggtgccttc acgcacgact
     6061 gggggtttga ggtcagcgtt tactataacc gggtgagtga tgccatcctc tcgcacaata
     6121 tcgatgccga taccattcaa aatcagaaca gcggcacggt ggattacagc ggtctggatg
     6181 ccggtattaa ggggaaaatc agcaatatac tggatgtagg attgagctac gccctgatcc
     6241 acgctgatgc caaacgcaaa gacatcggca agataaccga tctgccaacg cagacaatga
     6301 ccgcatggat gactctcaaa ccgtgggagc cgttaagcgt aacgctgtcg gaagaggcgc
     6361 gttcctccag ctacagcaac agtgacggtt cacaaaaagc cgccggtttt gcggtgaccc
     6421 acattcgagc cgattacacc ttaggtcatg gcttcagcgt caatgcgtcg gtcaataacc
     6481 tgtttgatac ccaatatgcc tacagtgaag ggtttattga agaaggtcgc aatttctggg
     6541 caggtatcga gtatacgttc tgatgtgaaa gcggatagcg gcatctgtat cgctatccgc
     6601 cttatgaaaa tcttattctc ttcgcaggag gcggaatacg cccagcacca gaatagcgcc
     6661 cactaccgcc accaggaagc tgtgcaaatt aaaaccgctg atggagccgc caatgccaaa
     6721 catggtcgcc agccagccgc cgaccaccgc accgactatc ccgagaatac aggtcaggaa
     6781 aaatccacca ccatcacgcc ccggcatgat tagcttggcg ataatgccgg ctatcaggcc
     6841 aaaaataatc caggcaataa ttcccatgtt caggccctcg tttcgatgaa tcggacgcaa
     6901 gaaatgctta cgtcaacgct ctaagtatag gcaagggcgg aatcgatgct gggcgtatgt
     6961 cggctgattt aagaacaaca tgtaaaaaat attcacgcat ttgtattaag ttttgttaac
     7021 tgtgaccgat aaaccaaaga cagtttgtca gtcaggagtt tttccgcgtg agtcattacc
     7081 atgagcagtt cctgaaacaa aatccgttag ccgtcctggg cgtgttacgc gatttgcaca
     7141 aagccgcaat tcctttgcgt atcagttgga atggcgggca gctgatcagc aaaatactgg
     7201 caataacccc ggataaactg gtgctggatt tcggcagcca ggccgaagat aacaacgctg
     7261 tgttaaaggc gcagcacatt accattaccg ccgaaaccca gggcgcgaaa gtcgaattta
     7321 ctgttgaaca actacagcag agtgaatact tgcagcttcc ggcatttatt accgtaccgc
     7381 ctcccacctt gtggtttgta caacgacgcc gatatttccg catctccgcc ccactccatc
     7441 cgccttattt ttgccagacc aaactggcgg ataacagtac gttacgtttc cgcctgtatg
     7501 atttgtcgtt aggcggcatg ggcgcattac tggaaacagc aaagcctgcc ggattgcacg
     7561 aaggcatgcg cttcgctcag attgaagtca acatggggca atggggtgtt tttcactttg
     7621 acgcccagtt aatctcaatc agcgagcgca aagtgattga tggcaagaat gaaaccatca
     7681 ccactccccg tctgagcttc cgttttctta acgtcagccc gacggtggag cggcaattac
     7741 agcggattat tttctctctc gagcgggaag cccgggagaa agcggacaaa gtgcgcgact
     7801 gatttacatc gcgtccagtg cctgctcaag tttgtagata tagcgcggag cctgcggcgc
     7861 aggatgattt cgcgctacgt gatcgaggaa ctcgtcagca tccagatcgt tgattttgct
     7921 gatcgccttt ttccgatctg acgagaaagt ccgtagcagc gcacctgccc cgttagcgta
     7981 tgacaccacc agcgcatatt gcagtacctt cggatcttca atacctgcca gcgggccggt
     8041 ttccagaata ttcaggtaag ccgcccccat tgaaatatta cgctccggat ttttcagctc
     8101 gctggtcgtc ggctcaccac tccagcccat acggcgataa acatcacgtc cggaggttga
     8161 agcttttaac tgcatcaaac caatggcatt cgatttactc accgcattcg gattaccacc
     8221 cgattcgata gcgataatcg ctgtgatcaa ttgtggatcg acgccccagg ctgcaccggc
     8281 tttctggctt attggcatcc actgcatcgc acgttgcacc ggaactttcg cgttccacgg
     8341 cgggttcgtg tagtcatgct ttgatgaaca acctgctaat aacacaatca aaaaggcaaa
     8401 ccatctcaat ttcacttctt ctatccttat agcctggaat cgccgttgag gtgacaacgg
     8461 cacacctaag cggcatgata tacatttcgt ttctgttgtc agcaaggaat tgccatgtct
     8521 ctgtttcact taattgcccc atcgggttac tgcattaaac agcacgccgc gctgcgtggt
     8581 atccagcgcc ttaccgacgc ggggcatcag gtcaataacg tcgatgttat tgcccgtcgt
     8641 tgtgagcgtt ttgcgggtac ggaaacggag cgtctggaag atctcaattc ccttgccaga
     8701 ctgactaccc ccaacactat cgtgctggct gtacgcggtg gttacggtgc cagtcgttta
     8761 ctggcggata ttgactggca ggcactggtg gctcgccaac aacatgaccc gttgctcatt
     8821 tgcggacata gcgattttac cgccattcag tgcggtcttc tggcgcatgg caatgtcatc
     8881 acctttagcg gccccatgct ggtagcaaac tttggcgcgg atgaactgaa cgcctttacc
     8941 gagcaccatt tctggctggc gttacgcaat gaaaccttca ccattgaatg gcaaggggaa
     9001 ggcccgacat gtcagaccga aggcacgtta tggggaggca atctcgcgat gctgatttca
     9061 ctgattggta caccgtggat gccgcaaatc gaaaacggta ttctggtgct ggaagatatt
     9121 aacgagcacc ctttccgcgt cgaacgtatg ctgttgcagc tctatcatgc tggaattttg
     9181 ccacgtcaga aggcgattat tctcggtagc tttagcggca gcacgcccaa tgattatgac
     9241 gcaggttaca acctggagtc agtgtacgcg tttttgcgtt cccgcctgtc gattccactc
     9301 attaccggcc tcgattttgg tcatgaacca cgcacggtta cgctgccgtt gggcgcacat
     9361 gccattctta ctaacacccg ggaaggcact cagttgacga ttagcggtca tcctgttctt
     9421 aaaatgtaaa aaaatcacgc ttcagggcta agtaaaacgc tgtctctgcc gctaatatgg
     9481 tagggtttat ataagagaca gcgtaatcag gagtaaccga ccgttggatg ccacaacaat
     9541 aattagcctt ttcatcttag gatccatcct tgtcaccagc agtatattac ttagttcatt
     9601 ttcttcccgt cttggcattc ctattctggt tatttttctg gcgatcggca tgctggcggg
     9661 agtcgatggc gtcggaggta tcccgtttga taattacccc ttcgcctaca tggtaagtaa
     9721 cctggcgctg gcgattattt tgctcgacgg cgggatgcgc actcaggcca gttcctttcg
     9781 tgtggcgtta ggaccggccc tgtcgctggc gacgctgggc gtgcttatca cctctggttt
     9841 aaccggcatg atggcggcgt ggctgtttaa tcttgatttg attgaaggct tattaatcgg
     9901 cgctatcgtc ggctccaccg atgctgcagc ggtcttttct ctgctgggtg gtaaggggct
     9961 taacgaacgt gttggctcga cgctggaaat tgaatccggc agtaatgatc caatggcggt
    10021 ctttctgacg attaccctga ttgcgatgat ccagcaacat gaaagcagtg tcagttggat
    10081 gttcgtagtc gatattctgc agcaatttgg cctcgggatt gtcatcggtc ttggcggtgg
    10141 ttatttactg ctgcaaatga ttaatcgcat cgccctgccc gccggattat atccattgct
    10201 ggcattaagc ggcggtattt taatttttgc tttaaccacg gcgctggaag gtagcggtat
    10261 tctggctgtt tatctgtgcg gttttctgct gggtaatcgc ccgattcgca accgctatgg
    10321 catcctgcaa aatttcgacg gcctcgcctg gctggcgcaa atcgccatgt tcctggtgct
    10381 ggggctattg gttaacccaa gcgatctgct gcctattgct attccggcgc tcattttgtc
    10441 cgcatggatg atattcttcg ctcgtcctct ttcggtattt gccggattgc taccgttccg
    10501 tggttttaat ctgcgtgagc gcgtgtttat cagctgggtg ggattacgcg gcgcggtgcc
    10561 aatcatcctg gccgtgttcc cgatgatggc ggggctggag aatgcgcgac tgttctttaa
    10621 tgtcgccttc tttgtggttc tggtttcact gctcttgcag ggaacatcac tctcgtgggc
    10681 ggcgaaaaaa gccaaagtgg tcgttccgcc agtgggacgt ccggtgtcac gcgttggtct
    10741 ggatattcat ccggaaaatc cgtgggagca gtttgtttat cagttgagtg ccgataaatg
    10801 gtgcgtgggc gcggcactgc gtgatctgca tatgccaaaa gagacgcgta ttgcggcact
    10861 gtttcgtgat aaccagttgc ttcatcctac cggcagcacc cgactgcgcg aaggcgatgt
    10921 gttgtgtgta attggtcggg aacgcgatct cccggcgctc ggtaaactgt tcagccagtc
    10981 gccgccggtc gcgctggatc aacgcttctt tggtgacttc attctcgaag ccagcgccaa
    11041 atatgcagat gtggcgctga tatatggcct ggaagacggg cgagaatatc gtgataagca
    11101 acaaacgctg ggtgaaattg tccagcagtt gttaggcgcg gcaccggttg tcggtgacca
    11161 ggtagagttt gccggaatga tctggacggt ggccgagaaa gaagataatg aagtgttgaa
    11221 gattggcgta cgggtggcgg aagaggaagc tgaatcttaa atttaataca gcatctatcg
    11281 ccggatgcga tgctttgcga acgcagcctg agcctgcata taaacttgtc atttcctgat
    11341 tttgatatgc tcataccaac atcagaagaa ggttaaatta tggatgacaa accattgcag
    11401 gctctggcta atcaactcaa aacgccggct caagaaagtc agcataatgg cttcttctga
    11461 ctgcaacagg tgaagaataa ccagccccgc attaccggta tccccgtcaa ccatcagggg
    11521 ccccagcctg acatcgcctg aacatattcc cccacgcaac ctgccgcact ggcgtagccc
    11581 acacaatacg cgtttcagac tgctcagtaa ctgtagtggc gtttcatccg cctgtagcac
    11641 cggatgcatg agcgatttct tatagttatt cctttgctta ataacgatta tttccgggtc
    11701 gttcgctctc gtattttctt taatacgtca gagctgcctg ggccgtaata cagcatgtac
    11761 cagacccgac acggcatttc tgaccaaggg aaaaagtcaa cgatgagtcg ttatatcagc
    11821 gcaaatgacg tcctgtggca cctttgcggt gaccacattc acgtctccgg cgctgccaga
    11881 ggatatttat ttcaaggata cgtagcatta tggccagcat taaagacatc cgcccattcg
    11941 cccccggcat tcccccatct tccgttaacc caggcgtacg cccaccaggg cagttcttcc
    12001 tgccggtgga actcaagaat gaaattttgc ggaacatgaa gtatatcggc ccgcaaatat
    12061 ttagctgggg tgatgaccac cggcagatca ttgacctcag taccgcgggc gaagtgaaaa
    12121 tacatatggt agacaatacc atcatcacgt tggagcgcct gaatccgaat ccgcaacata
    12181 acgagttcat gatggtcgcc gaatggtgcc aatgggctat ggagcgggat ggtttgcaag
    12241 aaaacctgca aaaaaacctg tacgaaatcc tggaagaaaa ccagcaaaac aaacagtcag
    12301 aaattcccca agaagacctg aaagaaagtc tggaagaaat caaggaaaat atcctggaag
    12361 aaaacctgcc agcaagccgg attgaaaacc gggcagaagc attgcgcaga atgaaggaat
    12421 gtctcattac aagacagagt atgctcaatc ttagcaacct gggattgact tcactccctg
    12481 agaatttgcc tccacatctg attgaatttt actgcagtaa aaacgtgttg accgcgttac
    12541 cgaaggtgat gccaaagtgg ctgctggtgc ttgactgtac agataacgtc ttgatcttgt
    12601 taccgaaggt gcagccctca aaactgatgg tactgaagtg ctatgaaaac tgtattatct
    12661 ggctgcctga gctgtcgact aacctgagag tgattaactg ttctgaaaac ttcttgcaat
    12721 ttttaccgcc gtcgatgccc cagtacctgt ataaactatc ctgtgccggc aacaatattc
    12781 actcaatacc tgatgagatg ctggagaact tgactcgcct gaaagtattt gactgttcta
    12841 gtaacgattt gatctctgca ccacggctgc cgcccaaact aataatttac tactgtggag
    12901 aaaacaagtt taaaacggta caggtgccac agccccagag cctgaaggtg tttgactgta
    12961 atggtaaccc gtgggacaaa gacaatttac cgacgctgtt caaagccgtc gagggcctga
    13021 aaaaacagca gggcctgaag gaccttttgg gctttttgca caaggagggc cagatgaagc
    13081 tggagaagct ggagaagctg gaggatctgg atgaccttat ggatctagag ctcctggatg
    13141 acccggtact cctggagcgc gtgaaggtac aggaggacct ggagctcctg aatcagcagt
    13201 tgggcctgtt gattctggaa agacaacagg actcgcagcc tgttaatcaa cattctgaac
    13261 atgaacccga atctgcatca agggtgaagc gtgatttatc tgaagtcgag ttcgagtcaa
    13321 caatgaaacg taagcgtttt atgtagaacg cctgtgcggt gaactcaggt tccggggaaa
    13381 tagtgtccat cattgtaatg gacactatca tgggaaatgg ctctgtcaca tcaacaacta
    13441 cagttgctga ccagccggat ggatgatttt ttcgcaacca gaagacgctg cctccgatcc
    13501 ggcttacaac aagttacact gtcataaccg ggacacgtaa cgccagcgcg cacatcaact
    13561 catagcccac cgttccggca gcggcggcga catcatcaat tttgatctcc ttgccccaca
    13621 gctcaaccgg cgtaccaatc cccgcctgcg ggcaaggcgt taaatcaacc gccagcatat
    13681 ccatcgagac ggtccccacc gtcatggtgc gcacgccgtc cactaaaaca ggagtaccgg
    13741 taggcgcatg gcgcggataa ccgtcggcgt atcctgcggc gacaatgccg attcgctgtt
    13801 catcgcgcgc agtatagcga ccgccgtagc ccacacgctc gcccgctttt agcgtctgga
    13861 caccaataat ctcactgctt agcgtcatca ccggacgtaa tccggtattg gcgatatcac
    13921 gccactgacc ggacggcgaa gcgccataca aaataatgcc aggccgaacc cagtcaaaat
    13981 gcgcttccgg gtgccacaga gtcgccgccg aattggacaa cgaacgccga cactccagcc
    14041 cttccgccgc ctgctcaata cgctccatcg cgccggaaat tccatcagga tgttccgcct
    14101 cggcaaaatg cgacatcagg gtcatttcgc caacattcgc catcgcccgc aactgctgcc
    14161 agacggtaag tacgcgatcg ggctggaagc ccaaccgatt catcccacta ttcactttaa
    14221 gataaatatc caacggtgct tttagccgcg cattttgcag tgctttgagc tgccagttgc
    14281 tgtgtacgca ggtggtcagg cggtgctggt cataaatctc cagatcctga gcatggaaaa
    14341 atccttccag catcaggatc ggtcctttcc agccgcgctc acgtaacgtt attgcctctt
    14401 ccaggttaag taatgcaaag ccatcggtgg ccccgagcgc gctccagata cgctcaatac
    14461 cgtgcccgta agcgttcgct tttaccactg accagacgcg cgcgtgcggc gcggcctggc
    14521 ggacaatgga cagattctgt tttaatgcct gcagatcgag gctggcctgt atcggacggg
    14581 tcatctcgtt tccttagctg tgcgcgccat gtaaatggcc cgggcgtgat ggcgtaaatc
    14641 cacggctgta gcgcgctacg cttagatcct catacggaat cgctggcgtg cgaccagaga
    14701 gcagatcgct taacaactga ccggaaccgc aagccatcgt ccagccgagc gtgccgtgac
    14761 cggtattcag ccacagattt ttaaagcgtg tacgcccgac aaccggcgtg ccgtctggcg
    14821 tcatcgggcg cagacccgtc cagaaagtcg cctgctcgac atgaccgccg cgtggataga
    14881 gatcgcgaac caccatttcc agcgtttcac gacgcggttg caacagctcg gtgttaaaac
    14941 caacaatttc cgccattccg ccaacacgaa tgcggttatc gaaacgggta atggcgattt
    15001 tgtaggtttc atcaaggata gtagacaccg gcgcaccatc ttcctgcgca atcggaatag
    15061 tcagcgagta gcctttcagc ggatacaccg gaatatcaac aatgcctttg agcatcgccg
    15121 tcgagtaaga accaaatgcc atcacatatg catcggcctt aatcacttca tcgccacact
    15181 tcacgccgta gatttgctcg ccgtcgcaaa gcagttggtc aacgggcgta ttaaagcgga
    15241 atttaacccc cgcctgctcc gccatccgcg ccagattctg ggtaaacagc tggcagtctc
    15301 cggtttcatc attgggcagc tggagccccc ccgtcagttt gtgcgctact tctgccagcg
    15361 cgggctccac ttccgccagg cggctggatt ccaacagttg atacggtacg ccagcatctt
    15421 ccagcacggc gatatcgcgg gtcgcatttt catactgttg ttcggtacgg aacagttgca
    15481 gcgtcccacc ctgacgccct tcatactgaa tattggtttc ggcgcgcaat gctttcaggc
    15541 aatcacggct gtattccgcc agacgcacca tccgcccttt gttttccatg tagtggctgg
    15601 tgtcgcagtt acgtaacatt tgccacatcc atttcaactg gaactgcgta ccgtcgagac
    15661 gaaccgccag cggcgcatgg cgctggaaca tccatttaat cgcttttaaa ggcacacctg
    15721 gtgccgccca cggtgctgca tatccggggg agatttgccc ggcattcgcg gcactggttt
    15781 ccagggcggc ccccggctct cgatcaatga cggtgacctc atgtcctgcc tgatttaagt
    15841 accaggcgct ggcaacgcct accacaccac ttcccagtat gacaactcgc atagccactc
    15901 cgataacggt agatacagta aaagaataat aatctaatta catcttgata actcagttga
    15961 aaatattatt caacatacgg cttttttatg gtgagctggc tcacatctcc ctgactctgc
    16021 ggcaatatga cgtctttagt atctcctgct gaacgccctt tttatccagc ataaaattcc
    16081 gttcagaagc ggattagtgg cactctgata ataagaaatc gcaaagaaaa aaattctccg
    16141 gcgacgcggt ttttaacagg tgttctatgc ttgaaatgag gtgctccaca cagagagcgc
    16201 cgacaacaat gaggtgcgcg tatggcgacg atcgattcta tgaataagga caccacacgt
    16261 ttgagcgatg gacccgactg gacgttcgac ctgctggatg tttatctggc agagatagac
    16321 cgggtggcga aactctaccg gctggatacc tacccgcacc agattgaagt gataacctca
    16381 gaacagatga tggatgccta ctccagcgtc ggcatgccaa ttaactatcc gcactggtca
    16441 ttcggtaaaa agtttatcga gactgaacgg ctgtataagc acggtcagca aggactggcc
    16501 tatgaaatcg tcattaactc taacccgtgt atcgcttacc tgatggaaga aaacaccatt
    16561 accatgcaag cgctggtgat ggcacacgcc tgctatgggc ataactcttt cttcaaaaac
    16621 aattacttat tccgtagctg gaccgacgcc agttcgattg tcgattatct gatttttgcc
    16681 cgtaaatata ttaccgagtg cgaagaacgt tatggcgttg atgaagtaga acggcttctg
    16741 gactcgtgcc acgcgctgat gaactacggc gtggaccggt acaaacgccc gcaaaaaatc
    16801 tcgctgcaag aagagaaagc ccggcagaaa agtcgcgaag agtatctgca aagtcaggtc
    16861 aatatgctct ggcgtaccct gccgaagcgc gaggaagaga aaacggttgc tgaagcgcgc
    16921 cgctatccgt ccgagccaca agaaaacctg ctctatttta tggagaaaaa tgcgccactg
    16981 ctggaatcat ggcagcgtga aattctgcgt attgtgcgta aggtgagcca gtatttttat
    17041 ccgcaaaaac agactcaggt gatgaacgaa ggctgggcga ccttctggca ctacaccatc
    17101 cttaaccatc tgtatgatga agggaaagta acggaacgtt ttatgctgga gtttttgcac
    17161 agccacacca atgtggtctt ccagccaccc tataacagcc cgtggtacag cggcatcaac
    17221 ccgtatgccc tcgggttcgc catgttccag gatattaaac ggatttgtca gtcgccaacg
    17281 gaagaagaca aatactggtt cccggatatc gccggttccg actggctgga aacgctgcat
    17341 ttcgcgatgc gtgatttcaa agatgagagt tttatcagcc agttcctgtc accgaaagtg
    17401 atgcgtgatt tccgcttctt caccgtgctg gatgacgatc ggcataatta tctggagatt
    17461 tccgctattc ataatgaaga aggttatcgg gagatccgta accggttatc gtctcaatat
    17521 aatttaagta atctggagcc gaatattcag atctggaacg tggatttgcg cggcgaccgt
    17581 tcgctgacgc tgcgttacat tccacataat cgcgcaccgc tagatcgggg gcgcaaagaa
    17641 gtactgaagc atgtgcatcg cctgtgggga tttgatgtaa tgctggaaca gcaaaacgaa
    17701 gacggcagcg tcgagttgct ggaacgttgc ccgccgagaa tgggaaatct gtaaaaacaa
    17761 caaaacccct cgtttgaggg gtttgctttt taaacggaag ggattatcgc ccctgaatgg
    17821 ctaaatcacc cggcagattt ttctgcatcc ggtgccaaat ctcgccgctc tcatgcccat
    17881 agcgacgcac tgtttcgtat acctgatcgt gtgcgccttc actgcacaat gccgacagtt
    17941 tgtggtagaa acccagcgcc agactgcgcg cttccggatt ggcgaaatag tgacgaccaa
    18001 tgcgcgtata cagccctttc atcccgttaa ggatcagacc gtaaatcggg ttgccggaag
    18061 caaacgccag accgcggaat atgttgtaat ccagctcggc aaaggcatcg gcgtgatcgg
    18121 ccacttcatt agcggttgcc agtacttcct gcgctttatc aggatgctga cgaaacgcgg
    18181 tgcgaataaa aatcgttgaa atattggtac gcaccgacag caaattatca ataagctgcg
    18241 gcacactttc gtgatccagt cgtgccagtg tttcaaggat atttaaaccg gaggtttccc
    18301 agaaattatt caccttcgtc ggcttgccgt gttgaatggt caaccagcca tctcgtgcca
    18361 gacgttgtaa cacttcacgt aacgtagtac gcgttacgcc aattaattct gaaagttcac
    18421 gttctgcggg caaaatagtc ccgggaggga agcggttatt ccagatactt tcaataatgt
    18481 actcttccgc gaaacccgcc gggctttgcg ccttaatgac catagtgaga tttccataac
    18541 acagcaaaac aaagttggac tcatcatacc agagggctaa ctacgctgat agcagacaaa
    18601 aagaagaaaa aagggatcaa ggcttcattt tccgcatctc tttacccttg actatcaaga
    18661 cttgcctcac tcctgcctga ccgttaagct ttgcgtttaa acataaaaac atcattgatt
    18721 atttgtaagg taagggaaac catcatggag atctcctggg gccgcgcgtt atggcgcaat
    18781 tttttgggcc agtcccccga ctggtacaaa ctcgccctca tcattttctt aatcgtaaat
    18841 ccgttaattt tcctcatcag ccctttcgtc gctggctggt tgctggtagc ggaatttatt
    18901 ttcactctgg cgatggccct gaaatgctac ccgctactcc ccggtggcct gttggctatc
    18961 gaagcggtat tcatcggcat gaccagcgcg gaacacgtcc gtgaagaggt ggcggcaaat
    19021 cttgaagtct tgctgttact gatgtttatg gtggcgggta tctattttat gaaacagctg
    19081 ttgctgttca tatttacccg tttgctgtta agcattcgct ccaaaatgct gctgtcgctc
    19141 tctttttgcg tggcggcggc gttcctctcc gcgttcctcg atgccttaac cgtcgtggcg
    19201 gtggtgatca gcgttgcagt cggtttttat gggatttatc accgcgtagc ctcttcccgt
    19261 accgaagata ccgacctgca agacgatagt catatcgaca agcattacaa agtggttctg
    19321 gaacagttcc gtggctttct gcgtagcctg atgatgcatg ccggtgtcgg caccgcatta
    19381 ggcggcgtaa tgaccatggt gggcgaacca cagaacctga tcatcgctaa agcggctggc
    19441 tggcattttg gcgatttctt cctgcgcatg tcgccagtga ccgttccggt tctgatttgt
    19501 ggcctgttaa cctgcctgct ggtagagaag ctgcgttggt ttggctatgg tgaaacgctg
    19561 ccggagaaag tccgcgaagt actgcaacag tttgacgatc aaagccgcca ccagcgtacc
    19621 cgtcaggata aaatccgtct gattgtccag gcgattattg gcgtctggct ggtgactgcg
    19681 ctggcgttgc atctggcgga agttggcttg attggtttgt cagtcattat tctggcgaca
    19741 tcattgaccg gtgtcaccga tgaacatgct atcggtaaag ccttcaccga atctctgcca
    19801 ttcaccgcac tgttgacggt gtttttctcg gtagtcgcgg tgattatcga ccaacaactg
    19861 ttttcgccga ttattcagtt tgtgttgcag gcatcggaac atgctcagct gtcgctgttc
    19921 tatattttca acggtctgct gtcatccatt tcggataacg tcttcgtggg gacgatttat
    19981 atcaacgaag cgaaagcggc aatggaaagt ggcgctatca cgttgaagca atacgagctg
    20041 ctggcggtcg ccattaatac cggtaccaat ctgccctctg tcgctacgcc gaacggtcag
    20101 gctgcgttcc tgttcctgct gacctctgca ctcgcgccat tgattcgcct ctcttatggc
    20161 cgcatggtgt ggatggccct gccttacacc ctcgtcctga cactcgtcgg actactttgc
    20221 gtcgagttta cgcttgcccc tgtaaccgaa tggtttatgc aaatgggctg gatagcaacg
    20281 ctttgataac aacttaccgg gcatataaat gcccggtttg ccttttcgcc cgataattgt
    20341 ccaattgcgt cctatttttg ctgcctcctg gtggcgcagc gaatgaattg gtttaaactg
    20401 cgcactctat gcatattgca gggaaatgat tatgttgcga tttttgaacc aatgttcaca
    20461 aggccggggc gcgtggctgt tgatggcgtt taccgctctg gcactggaac tgacggcact
    20521 gtggttccag catgtgatgt tactgaaacc ttgcgtgctc tgtatttatg aacgctgcgc
    20581 attattcggc gttctgggtg ctgcgctgat tggcgcgatc gccccgaaaa ctccgctgcg
    20641 ttatgtagcg atggttatct ggttgtatag tgcgttccgc ggtgtgcagt taacttacga
    20701 gcacaccatg cttcagctct atccttcgcc gtttgcgacc tgtgatttta tggttcgttt
    20761 cccggaatgg ctgccgctgg ataaatgggt gccgcaagtg tttgtcgcct ctggcgattg
    20821 cgccgagcgt cagtgggatt ttttaggtct ggaaatgccg cagtggttgc tcggtatttt
    20881 tatcgcttac ctgcttgtcg cggtgctggt ggtgatttcc cagccgttta aagcgaaaaa
    20941 acgtgacctg ttcggtcgct aatccattca gcgctcctgc gggagcgctt ttttcctgcc
    21001 gctatgttta tttgacccgc agtaaatcag aacttcgcgt tgtataacgt ggtgaaagca
    21061 tagctcgctt catctgccat tgttgttgga tcccctgccc ggcaaaatag agtgttcctc
    21121 tgccctcttt agcattcaga gtatccatta ccgccatcaa ttgctcactc ccggggggcg
    21181 gtgcgttgtc atcgaataaa ttgagctgcg cgactccctg actgaagaaa tctcccagca
    21241 tcacgcccgc tttttggtag cgatggcccg cttgccagat ggcatccaga gatcgcgtag
    21301 cggcgttaat gatatccctg ctgtcctgag tgggcgtcag cagttttacc gacgcgctat
    21361 tgccgtaata aggttcattg agcgcaaatg gtgacgtctt aataaacgtg gaaataaacc
    21421 gacaatattg atgctcactg cgaagttttt ccgccgcccg ggcagcgtaa ctacaaatgg
    21481 cctgccgcat cgacgtataa tccgtgatgc gttcaccaaa cgaacgggaa cagataattt
    21541 cctgcttcgt cggtgcaaac tcttccagtt gcaaacaggg ttcaccgtgc agttcacgca
    21601 ccgttctttc gagcacgaca ttaaaatgtt tacggataaa ccggatatct gtacccgcca
    21661 aatcgagaac ggttttgatc cccatcgcgt ccagtttttt gctgatccgc cgtccaatcc
    21721 cccagacttc atccacgggg agagcagaca ttaatttacg ctggcgttcc agatttgata
    21781 aatccaccac cccacccgtc tgccgctgcc atttttttgc cgcatgattg gcaagcttag
    21841 ccagcgtttt ggtctgggcg atccccacgc caacagtaag atgggtacgt tgtagcaccg
    21901 ttgcgcgaat ttctcggcca aaatcagtca gatcgcgaca attacgcaca ccggtcagat
    21961 cgcagaatgc ctcatcaata ctgtaaatct cgacgcgggg cgataattct tccagcgtcg
    22021 acatcacccg attgctcata tcggcgtaaa gctcatagtt actgctaaag caaaccacgc
    22081 cacagcggcg aaacagatct ttttgtttga accagggatc gcccatttta acgcccaacg
    22141 cctttgcctc agcgtttcgg gcgataacgc aaccgtcatt attcgatagc acaaccaccg
    22201 gtttacccca taaatcaggg cgaaacaccg tctcacagct ggcataaaac gcgtttacat
    22261 cacagagggc aaacatcagc gcatcgcctt aacgacgtgg atcaccacac caaagacatc
    22321 cagcgtatct tcgctactga tggtaatggg tgaatatgca ctgttcatgg gaataagctg
    22381 cactgtcggg cgtagttgca attttttcac cgtaaactcg ccgtcaacag cagcgatgac
    22441 aatatcacca tggctggcgg taatagcgct atcgacaatc agtaaatcac cgtcactaat
    22501 cccaccgtca atcatagaat caccacttgc tttgacgaag taagtcgcgc tgggatgctg
    22561 gatcaacagt tgattcagat cgatgcgctg ttcaacgtaa tctgcggccg gtgaaggaaa
    22621 gccacactga acaagatcgc taaatagcgg aaaagtcaca atttcgcgga gatccgcagg
    22681 cttgataaac aacataataa tctgcctgaa gttatactgt ttttatatac agtagtctgt
    22741 tcttgccagc agatcaatac tgattcaggc tatcaatatt tgtcgctgca tagactgctg
    22801 atttttcgtt ctcttatcct gtgctcacgc ggccttctgg cgacgacgct catccagcag
    22861 aaatgaaaaa tatcacccgg ctaaaaatag aatagaagca ttcgccataa tgacattaaa
    22921 cattgtttga tatttatcat attaatagaa ataaagacat tgacgcatct cgcccgccta
    22981 actatgaatt agatgaagta aaattcatta atagttgtaa aacaggagtt t
//
LOCUS       DACBVI010000064        22714 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.064, whole genome shotgun sequence.
ACCESSION   DACBVI010000064 DACBVI010000000
VERSION     DACBVI010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.064"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(941..1126)
                     /locus_tag="IU325_12115"
     CDS             complement(941..1126)
                     /locus_tag="IU325_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809767.1"
                     /translation="MDRKSNSDSYQRWMIEKADKRERTQISMSFVKMKDVLFNVLAIG
                     TVLAALVVIFYEVQSSL"
     gene            complement(1084..1296)
                     /locus_tag="IU325_12120"
     CDS             complement(1084..1296)
                     /locus_tag="IU325_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809768.1"
                     /translation="MVTQSLADFGRYSYKPRPAFQDWIERLKTGERLPRGRYFVGKKP
                     GGRLVLIDELGGTWTEKVTVIHTNAG"
     gene            complement(1359..1634)
                     /locus_tag="IU325_12125"
     CDS             complement(1359..1634)
                     /locus_tag="IU325_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809769.1"
                     /translation="MHTTNAIILCLVSYPCIVAWVIYKNRFVDYLFLTLLACLGSIFS
                     VVLYKQPDYSLTIYQAVAVGYAAVIMPTVLVLSVVNIFMGKAERTCK"
     gene            complement(1652..1906)
                     /locus_tag="IU325_12130"
     CDS             complement(1652..1906)
                     /locus_tag="IU325_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809770.1"
                     /translation="MGHYTIRTNDDEDQAIKKAQEATGQASASKTFMTAILELQRNRD
                     EMAQLRRELAQEKARSQELVSSVKQFRSSLNNLFDLADNP"
     gene            complement(2016..2837)
                     /locus_tag="IU325_12135"
     CDS             complement(2016..2837)
                     /locus_tag="IU325_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sprT domain-containing protein"
                     /protein_id="HAP5809771.1"
                     /translation="MTRATERAYSELQQAFDFYNQRLFDGELPDCLITFQRGKNTMGY
                     FSYRRFVAADGSGRMIDEIALNPEYFPVYPLIEVMQTLVHEQCHMWQYHYGNPSRKTY
                     HNAQWAAKMESIGLMPSSTGRPGGAKVGQKINDYPIPGGRFQRVTLELFQGQFALSWF
                     DRFPVQVEQQKDMTAVIEQWRETLALAHQNAEAGIDIEAVLSMALLPSVSPQNGSHSD
                     DMAGSNDNDLSVAAFEDKPKRNKVKYQCRGCGAAVWGKAGLNIECGDCELAFIEN"
     gene            complement(2834..3013)
                     /locus_tag="IU325_12140"
     CDS             complement(2834..3013)
                     /locus_tag="IU325_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809772.1"
                     /translation="MTEKITDEELVDLLEALKRAHGMGVCSKAVKLAQRCADVFPAIV
                     AELQEYRNAAKRTSA"
     gene            3286..3936
                     /locus_tag="IU325_12145"
     CDS             3286..3936
                     /locus_tag="IU325_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HAP5809773.1"
                     /translation="MDIKPNSVIHLEPGETLPGYTNLKPVPDKYFDELKALINKLNTQ
                     SGNIYLKLKQMYAFLDRFNKEFVSTFTSCQKGCSSCCKMDVHLTALEATHIAQASKLT
                     ARDNPLTTGHESKCPFLSEKGTCSIYNYRPLLCRTYHVLTPPEMCNDLDAQVMQYGSQ
                     SANMGNHIYKTIAEWIYFQTYHCTGKLETKDIRDYFPYPREDIQRFLHHNPPRPFC"
     gene            complement(3974..4429)
                     /locus_tag="IU325_12150"
     CDS             complement(3974..4429)
                     /locus_tag="IU325_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HAP5809774.1"
                     /translation="MSENETFDATKKLLLNIRSVRVFARETSFEQLLEMQEKLNAVIE
                     ERREEAEREAAERAERERKRQELLQLIAGEGFSPEELLGLSEDAQKTRKKTLPKAPPK
                     YQFDENGETKYWSGRGRTPKPIDEALKAGRSLDEFLIKKDASSTAGDEQ"
     gene            complement(4441..6768)
                     /gene="topB"
                     /locus_tag="IU325_12155"
     CDS             complement(4441..6768)
                     /gene="topB"
                     /locus_tag="IU325_12155"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP5809775.1"
                     /translation="MRLFIAEKPSLAKAIFEGLGGNPNTEKKNGYFEHGSDVVTWCYG
                     HMLELYDPQDYDDKYSNWRYSDLPIKAVYPPKYKIKPESEAQTRIILSLIDKADSIVH
                     SGDPDDEGCLLVDEILGYAGNKKPVQRLLVADLNLAPVQKSLSNLQPNDKFRGMTLSA
                     LARSLCDQGFGYNMTRGCTLKGREKGYDGVLNVGRVQSAVLGLVNMRTLANQNHTESF
                     YYDVFASLSMNGHQIKAKYQTTDDDQIDEKKRLISEAQAAHIAGRVTGKDAIVTIATT
                     KPENSKPPLPLNLSTLQQLCAKRFGYSAKDTLDVMQGLYETHKLLTYPRSDNRYLSDE
                     HYYQAGDIATAIAATVPELASAIVDMDKGQKHKAFNASKIEAHHAIIPTTKSGAGIQL
                     NEKERNVYNLVSVYFIGLFYPDAIRNKTKIHFDIKGDTFTATQSVLVQKGWEALGKDS
                     DDEDETEDAGTDGFDLSLLKFNDSGLCESADVDKKKTTPPRYFTESTLLAAMTSAAKF
                     IDDPALRKALEAKDEGSSDRGSIGTEATRAGILEKLAANTGLISIEKEKGYSELVWKT
                     TKQGQEFCAALPPEITKPDISALWAEKQSQIKAGELTVEEFIKENDEYVQGLIDELDR
                     NGISISSNATPCPVCNNGFLRKRKGQNGFFWGCSCYPECKTTFPDKDGKPDMEAKSRS
                     EGSMSRLEAPCPSCSKEIIIRPKGFFCSGCEFKIWSEVSGKKLTQNQVETLIKKGKTG
                     EIKGFTSNKTGKKFDAAIVLQDKTTGKLGFQFSKK"
     gene            complement(6772..8034)
                     /locus_tag="IU325_12160"
     CDS             complement(6772..8034)
                     /locus_tag="IU325_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP5809776.1"
                     /translation="MGKLTLVEQALRLHKEYYGEDNFGTVVKDCDYYPKVAEIFINRK
                     NRKIYSNLDIEPVASTVVHTGSSPVMAKAGRIKKLMFKDGSGAYRIHLGQNEVVHIIR
                     FILNSKVRMEYAVGTTKSMSMLHNLCEAGRAKLNRSLPRKGIWKIGCYDGVYYHGKAR
                     KEEIENALRFSVHPKFNELENDFNQFFSDIDFYTRYGQSGMRKVLFTGPPGTGKTTIA
                     KALGAKYQDKYVFVYADDYFKDVCYAAAQKKIPVIIIAEEVDELYRADAGTLSFLDGA
                     DTPRNLAGTYVIFSTNYPNRIDPRIRKRPGRIDRIISVGAFRTKAAAACAKMYLPDDI
                     NIDLKELGAVLDRTTPAEIKEIINIAIGMIRGTKNELTVDVIKNARAFLKGTLDLSVQ
                     EAEEDIEEREEIFKKNGAQPDYSSYLED"
     gene            complement(8117..8611)
                     /locus_tag="IU325_12165"
     CDS             complement(8117..8611)
                     /locus_tag="IU325_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="micrococcal nuclease"
                     /protein_id="HAP5809777.1"
                     /translation="MRHFFNVLSFAVALLPATLCAAQIQGKVIRVLDGDTIEVKTLPA
                     KIVVYEVPIRVRLINIDAPEKKQPFGRWSTNQLKALLAGQSVTVSYTQTDRYGRVLGR
                     VVTANGTEANRQQVLKGAAWVYDRYNTDNSLPALQREAQTQKRGLWADSNPVPPWEWR
                     HKQN"
     gene            complement(8608..8934)
                     /locus_tag="IU325_12170"
     CDS             complement(8608..8934)
                     /locus_tag="IU325_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809778.1"
                     /translation="MKIINVPDARSVRIAGIEIIDSVKSVASVFTEILLFMVWFPAIV
                     YGLYRFVIVLWDASLKPQSLIEFINFMVELYSYVLPSLAVIAVALVVVLSIFKRLSAI
                     WEGRKQ"
     gene            complement(9020..9334)
                     /locus_tag="IU325_12175"
     CDS             complement(9020..9334)
                     /locus_tag="IU325_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809779.1"
                     /translation="MKKTVTTAVLLCAFAAGTAHAEEKADPNDPCAMVLCLAGKLDGS
                     SPAECDPMYKSFMSIRKKNKHGFLPDHTADARKKKLNECPAADAGTVSKIISSFGRLK
                     NF"
     gene            complement(9422..9814)
                     /locus_tag="IU325_12180"
     CDS             complement(9422..9814)
                     /locus_tag="IU325_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP5809780.1"
                     /translation="MKKMLMVSVLFLSACSSPPEPPQVDWEQNPETVNTQLMDWQPTY
                     SVIKSDKVNSSWVKVIHNFRPENRLYDDAVFYSVAHSDSVIVETSNGTDFFTAKNWLR
                     ANGANGVIQYRYKMNCFSCRTTSVYLSR"
     gene            complement(9811..11646)
                     /locus_tag="IU325_12185"
     CDS             complement(9811..11646)
                     /locus_tag="IU325_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP5809781.1"
                     /translation="MSLKLPDKGQWAFIGLVMCLVTYYTGSVAVYFLNGKTPLYIWKN
                     FDSMLLWRIITESNIRTDIRLTAIPSLLSGMVSSLIVPVFIIWQLNKTDVALYGDAKF
                     ASDNDLRKSKLLKWEKENDTDILVGAYKGKYLWYTAPDFVSLGAGTRAGKGAAIGIPN
                     LLVRKHSLIALDPKQELWKITSKVREKLLGNKVYLLDPFNSKTHQFNPLFYIDLKEES
                     GAKDLLKLIEILFPSYGLTGAEAHFNNLAGQYWTGLAKLLHFFINYDPSWLGEFGLKP
                     VFSIGSVVDLYSNIDRELILSKREDLEGTKGLDENALYHLRDALTKIREYHETEDEQR
                     SSIDGSFRKKMSLFYLPTVRKCTDGNDFDLRQLRREDITVYVGVNAEDMSLAYDFLNL
                     FFNFVVEVTLRENPDFDPTLKHDCLMFLDEFPSIGYMPIIKKGSGYIAGFKLKLLTIY
                     QNISQLNEIYGVEGAKTLMSAHPCRIIYAVSEEDDAKKISEKLGYITTKSTGSSKTSG
                     RSTSKSSSESEAQRALVLPQELGTLDFKEEFIILKGENPVKAEKALYFLDPYFMDRLM
                     MVSPKLTELTASLNKTKKVLGVKGLKYPSKEKMLSVGELESEVLL"
     gene            complement(11649..12683)
                     /gene="virB11"
                     /locus_tag="IU325_12190"
     CDS             complement(11649..12683)
                     /gene="virB11"
                     /locus_tag="IU325_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HAP5809782.1"
                     /translation="MKDAENRHIVYEIIDEYFHHWLNEVDGLTEIAVNRPDELFVKVS
                     GKWQQHELKMDFKDCMSFAGAISDYHDGGSVTPEYPLRSVTLPGGERVQIVIPPATER
                     ETVSITIRKPSSVFIDHDTFVKQGFYSRLNQGGKFRDKEDELSSMFKDNCFEQFVPEC
                     LVKGKTMVFCAGTGAGKTTFANACLQYIPHHLRCISIEDTDEAKFRFHKNHVKLYYPS
                     EGESSTVSSASLLRSGFRMNPDRILMTEVRGAEAWDFLKGSSSGHAGNLTTVHESTPE
                     DAVLGLVQRCYMNPECQNLPYNIILRRVLSNVDVIMSIKYIDEEDNRFASGIYYRDIH
                     FQEYFEKLKE"
     gene            complement(12680..12877)
                     /locus_tag="IU325_12195"
     CDS             complement(12680..12877)
                     /locus_tag="IU325_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP5809783.1"
                     /translation="MITLDIKEFSMLLGIRESEIYHHIRKGIPINGVPFPKPLKQIKT
                     HRFNYEEVMRFIEDLKGKGEL"
     gene            complement(12879..14093)
                     /locus_tag="IU325_12200"
     CDS             complement(12879..14093)
                     /locus_tag="IU325_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein VirB10"
                     /protein_id="HAP5809784.1"
                     /translation="MSEQENKIPTAVEIEQALRERHQKEIEQNDKTDKGDEDDGKSVK
                     RLGIEKLKKSRKGLVIIVAAFLLLAAGVSVYYIPSIIRAMSSGDDKPASNAVSTGSVK
                     RATGLSDDVDPFNTREEPAKTEERKESSGKAETPPEKVQQNFSRALDVAYGGSSSASS
                     NSGGSSSTASNTRNEGDGSDKQAEVQPVNAGQPAQLSKITRVPYDPNLFIPENTAIKC
                     SLDRRFISDLAGKLVCTINEDVYSANRNVKLIEKGTAAYLMYKTGTFNHGQGAVFIAA
                     TKLRTRKEPFIDIPLIDTQAAGALGEAGASGWIDTHFADRFMGAMMVGMIPDVAQAAS
                     GAAKSNKDNQTDYTANSRQAFADIAREAFANSVNIPPTLYKNQGEIITLIVGQDLDFS
                     SIYKLKMVGTRR"
     gene            complement(14090..15019)
                     /locus_tag="IU325_12205"
     CDS             complement(14090..15019)
                     /locus_tag="IU325_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HAP5809785.1"
                     /translation="MKMNKGALIVALLLVSHVCHAAVTPAASRFDPRNQIVSYNPQNT
                     TVINSAVGYTTTLVFDEDETVISARTGFPQGWEVNKEDNLVYLEVKPVKQTVQKNSTD
                     DNGNQSSEVVSVALDPENDLERWRTNLFVRTTKRNYSMELNARTFRQPDKIAFVVNYQ
                     YPQERRKEQAEIEKKRIEALNKRQEEQAINRSLENAKSPKNWDYWKRVADGSQSISPD
                     YAYDDGRYTWFGFSPLKKIPSVFVMSGEQETLTNPVVKNSGSFTVVGVPVDQRFVLRM
                     GNQVVGVENRGFGKVRLPAGDTVSPNVEKEVIQ"
     gene            complement(15025..15753)
                     /locus_tag="IU325_12210"
     CDS             complement(15025..15753)
                     /locus_tag="IU325_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HAP5809786.1"
                     /translation="MGFKLSGLKLPGFNKKKEVTDSSTTFEEKNVLMQEKMNRIYKFG
                     GIAGIAVGVLSLLALNGALPLKSTVVEAYLINGVTGVAERLTSVKKENLSENEALAKY
                     FITQYVKRREGYNYFSLQHDYDYVLLYSAENVATDYNALINGNQSPKVIYNKAEKTAS
                     VQDNPSVIISPSSRRDDKDMGAYIRFKLTIRNVATGQDDYEYWNVRLTYRIEPQVEMA
                     SGDRNNNPLKFVVTSYVRDKEVKG"
     gene            complement(15948..17003)
                     /locus_tag="IU325_12215"
     CDS             complement(15948..17003)
                     /locus_tag="IU325_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HAP5809787.1"
                     /translation="MATSRYFQAAHDVIVKTLDQSLTGQLDNFSSIASQLGKYGISIY
                     ILWYAYTTIVGKQKTAVQDFLWNLCRFWLILVFVKNMGGWLDSATQAIDGLKETFAGG
                     DPWKWVDELWEKVQQVAAYLMSKDTSKYVKTDGAIASLLTYAGGIIALLLTSIVYLAA
                     EVTIKILTITAPLFIICLSFGFLRQMFNSWLQLIFSSCFIFLFYGLAIKAGMTFLNGI
                     LTISIANADELNLISTGAQAGVAGAFMAWIIWQAKTYASQLAGVGVEGAMQGAAAMGI
                     GAGVFGASRMGRNLLGMGKNAGIGAWKGVRRQDGGFNQSPGVSGKLGNLTGQGVNIGA
                     KRLRQAAIDAAKKKYGG"
     gene            complement(17015..17272)
                     /locus_tag="IU325_12220"
     CDS             complement(17015..17272)
                     /locus_tag="IU325_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HAP5809788.1"
                     /translation="MKKYGLVAALGLTFFLTGCEEVKSVDWWQSHVDDATKKVAECKK
                     SGSDSENCKNAKEGLFRYKQLHPKHVDYKDAFKNVLGKDGS"
     gene            complement(17282..18052)
                     /locus_tag="IU325_12225"
     CDS             complement(17282..18052)
                     /locus_tag="IU325_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HAP5809789.1"
                     /translation="MKKHIVAALIASVTVISGAQAGVPVAIDANPEWAVEAQRWTERL
                     KQWQDTVNHYQKQINAYKQELLTKTGIRDVQGLVQSAQSVSKELENIYDQGNSFIDDY
                     IKNPEATLSEQARSLLADYKVTNTCKGLGYTGDLVRGCEASFLSQLAGIEYGNKLESK
                     LREDNQEMADLIDQVKNAQDTKATQDATNAVSLASLKFNKLKFQYEMYRDKQRDLAEY
                     KEKMLQAAFQEKQLGAVNKETPVVDYKAAFEQQSYEMN"
     gene            complement(18062..20815)
                     /locus_tag="IU325_12230"
     CDS             complement(18062..20815)
                     /locus_tag="IU325_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP5809790.1"
                     /translation="MSTVFKGLTRPALIRGLGVPLYPFLGMCVICVLLGVWIHEAMYA
                     LILPGWYAIKRVTKIDERFFDLLYLRMQIKGNPLANKRFNAVHYAGSSYDAVDISKVD
                     NFMKLKDQSSLEELIPYSSHITDNLIVTRNHDLLATWQIDGAYFECVDEADLALLTDQ
                     LNTLIRSFDGKPVTFYTHRIRVRKEVRPVFDSKIPFVNRVMNDYYESLSAAEYFENKL
                     YLTVCYKPFSAEDKVTHFLSRKKGNKNIFEEPINDMNEICGRLSTYLSRFHSRRLGLY
                     EENNIVYSEQLTLFQKLLSGRWQKVRVTNSPFYTYLGGKDLFFGNDAGQITASDHARY
                     FRCIEIKDYFQETDAGIFDALMYLPVEYVQTSSLTPIDKQSAIKALDDQIDKLEMTDD
                     AAKSLLADLKVGLDMVSSGYISFGKSHQTLIVYADSPERLVKDTNIVTTTLEDLGLIV
                     TYSTLSLGAAYFAQLPGNYTLRPRLSSISSLNFAEMESFHNFFTGKEKGNTWGNNLIT
                     LGGSGNDIYNLNYHMTTEHQNYFGKNPTLGHTEILGTSNVGKTVVMMTKAFAAQQFGT
                     PESFPPERKLKKLTTVFFDKDRAAEPGIRSMGGAYFRVKEGEPTGWNPAALPPTKRNI
                     SFMKDLVRLLCTLNSEPLDDYQNRLISDAVERLMQRSNRSYPISKLRPLILEPDDTET
                     RRHGLKARLNAWVQGGEFGWVFDNPDDTFDVDNLDVFGIDGTEFLDNKVLSSAASFYL
                     IYRVTMLADGRRLLIYMDEFWQWINNEAFRDFVYNKLKTARKLDMVLVVATQSPDELI
                     KSPIAAAVREQCATHIYLANPKAKRSEYVDELEVRELYFDKIKAIDPLSRQFLVVKNP
                     QRKGESDDFAAFARLDLGKAAYYLPVLSASKPQLELFDEIWKEGMKPEEWLDTYLERA
                     NLI"
     gene            complement(20840..21130)
                     /locus_tag="IU325_12235"
     CDS             complement(20840..21130)
                     /locus_tag="IU325_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809791.1"
                     /translation="MLKLNKRYLTLSVFMAALMLCVAEPAFADDVSTKTTGFLQKIID
                     FLTDIRKPAITIIALVIGYIAIFSRQHAAWITPLIIGIIIFIVAPYLPDWLA"
     gene            complement(21114..21758)
                     /locus_tag="IU325_12240"
     CDS             complement(21114..21758)
                     /locus_tag="IU325_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP5809792.1"
                     /translation="MLSTTAFAALALQCAASVHPDTAHEVARVESGFNPYAIAEIIPK
                     VERKPGDKGVVSYFPKTKEAALQIVNQIESRNHRYSVGLMQITSTNFANFNTTAEKMF
                     DPCENLKVSEQILVDCYKRGGDILRGLSCYYSGNPETGTKPESDFNNTSYIQRIGFNP
                     PDNKKNWVVPSVKDAIRKENVTQSIKPKEVTVYPQYAMRGTVLDEKETNDVKTQ"
     gene            complement(21804..22040)
                     /locus_tag="IU325_12245"
     CDS             complement(21804..22040)
                     /locus_tag="IU325_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809793.1"
                     /translation="MTATRKKTATRRKPQRNTLYLPSASREELDKIALEIGYRRGRRI
                     SSSGVVQYLIKKYSSQAIEELINEDDEDDDDFDD"
     gene            complement(21991..22500)
                     /locus_tag="IU325_12250"
     CDS             complement(21991..22500)
                     /locus_tag="IU325_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusG"
                     /protein_id="HAP5809794.1"
                     /translation="MEWYVLQFTTTRFAAVFAHLERLNFSYFCPMETERYRRPDKIIS
                     YRERRLPLFPGYLFIQADFEEVHSTTITAIPYVQRFISFGGEPLPVPEDVMAELLYRQ
                     SHTTAQANLLRKSIPHDFAEILLMDNPQQRSMAFIHYITERSLTHKMKRKKNDCYPKK
                     NRNETQAPT"
BASE COUNT         5931 a         5556 c         4766 g         6461 t
ORIGIN      
        1 ttaccgatgc aagaacaggg gagtttcatg aaatgcctgt ttaggagtga ttaaaggtaa
       61 cttgtgcgca gaaggtcagg tgtgtggtgg ttagtacaga ctagtgaatg ttgtaactaa
      121 actatatttt tattaaaggc cggaggtggt gttcctccgg ccttaattac gtttccgtgt
      181 tcattagtcg atgaactggc ggatcagttc cagaatggca atcagcgcgt ttgcaagcgc
      241 cgctaccgcc ctcagaacga tgattaacag gttaatcatc cgttttctcc ataaatggag
      301 taaagacacc gtggcttgct cttacactgc ctgtcaccac ggcttctctt cgtcttgtca
      361 gttgtagaga gtctcactcc tgccagagca cagataacca gcaccacctg tattacctgt
      421 caggactttg aacctgttca tgccgcattt agcggaatgc agggagagac tcaccacaga
      481 gctaaccctc tgattatacg tttatctaac gttcaggcaa accgttaacg cacagtcact
      541 gcccgtcata ttgcataatc aggcttcacg gattatcata ctattgtcag ttgtagagag
      601 tttcccttcc gggctgagtt accagctcgc accggaaccg taaaaaagcc gacctcacca
      661 gtcggttttt ttacgtctgc ggtttcccgc ttcgtttaaa tctattttaa atgaataaat
      721 tgttctgcgg caaacccaac aacataagga cgttagggtt gatggttgcc gaaatcgcgg
      781 tgcgttacgt gaattctgag gttttgcgag ggggattggg cgcgagaatt tacgagagag
      841 cgccggaaca atgccgggat cacaaaccat cacccgtgaa agaaatcttc cggcattgcc
      901 cggtcaggta gtatgacatt cattgtcaac ggccatcaat ttataagctg ctttgcactt
      961 cataaaagat caccaccagg gctgctaaaa cagtaccgat agccagcacg ttaaaaagta
     1021 cgtccttcat ctttacgaac gacatactga tttgcgtcct ttcccttttg tcagccttct
     1081 ctatcatcca gcgttggtat gaatcactgt tactttttct gtccatgttc cccccaattc
     1141 atcaatgagc accaggcggc caccaggctt cttgccaaca aagtaacggc ctcgcggcag
     1201 acgttcgcca gtcttcagcc gttcgatcca gtcctgaaat gcagggcgcg gtttatagct
     1261 gtagcggcca aaatcagcta aagactgcgt gaccatgact gtttggcagc catgctttct
     1321 catatcacga aacgtatttt ccagtgcagc cagtgtattc atttgcacgt tctctctgct
     1381 tttcccatga agatattaac gacactcaaa actaacaccg ttggcatgat cacggcagca
     1441 tagccgacag caacggcctg atatatggtc aatgagtagt cgggctgttt atacaatacg
     1501 actgaaaaaa tgctccccaa acaagcaagc agagttaaaa acaagtaatc gacaaatctg
     1561 tttttataga taacccatgc aacgatgcag ggatatgaca ccaggcacag gataatcgca
     1621 tttgtggtat gcacgtttcg ctccttgtta tttaagggtt gtcagccaga tcaaacagat
     1681 tgttcaggct ggaacggaat tgttttaccg atgataccag ctcctgactc cgcgcttttt
     1741 cctgcgccag ttcacgccgt agctgcgcca tctcgtctct gttacgctga agctcaagga
     1801 tagccgtcat aaaggttttg ctggctgatg cctgtccggt cgcttcctgc gcctttttaa
     1861 tggcctgatc ttcgtcgtca ttcgttctga ttgtgtaatg ccccatgata cccctctttt
     1921 gctgtcattt ttgcgctgcc ggcgcgaacc cgggaagaaa ctttgctggc aaaaaacact
     1981 ggccaacaga tgtgaccggg aaaatttgct ggcaattaat tttcgatgaa agccagctcg
     2041 caatcgccgc attcaatatt cagtccggct ttgccccaca cggcagcgcc acacccgcga
     2101 cactgatatt taactttatt tcgttttggc ttgtcctcaa atgctgcaac tgacaaatca
     2161 ttgtcattgc tcccggccat atcatctgaa tggctcccgt tctgcggtga cactgacggc
     2221 agcagagcca tagaaagcac cgcttctata tcaatcccgg cctcagcatt ttgatgcgcc
     2281 agagcaagcg tttcgcgcca ttgctctatt acggcggtca tatctttttg ttgttcgacc
     2341 tggacaggga agcgatcaaa ccagctcaaa gcaaattgcc cctggaacag ttccagtgtg
     2401 acgcgctgga agcggccacc agggatcgga tagtcattaa tcttctggcc gactttcgca
     2461 ccaccaggtc tcccggtgga ggagggcata agtccgatac tttccatttt tgccgcccac
     2521 tgcgcattgt ggtacgtctt acgcgacggg ttgccgtagt ggtactgcca catatggcac
     2581 tgctcatgaa ccagcgtttg catgacttca ataagcgggt acaccgggaa gtattcagga
     2641 ttgagggcga tttcatcaat catcctgccg ctgccgtcag cagcaacaaa gcgcctgtaa
     2701 ctgaaatagc ccattgtgtt cttgccgcgc tggaacgtaa tcagacagtc agggagttcg
     2761 ccgtcaaaaa ggcgctggtt atagaaatca aacgcctgtt gtagctcgct gtaagcgcgt
     2821 tccgtggctc ttgtcatgct gaagttcgct tagcggcgtt ccgatattcc tgtaactctg
     2881 caacaatagc agggaacaca tcagcgcaac gctgtgccag cttaaccgcc tttgaacaaa
     2941 cgcccatacc atgtgctctt ttgagcgcct ccaacaaatc taccagttcc tcgtcagtga
     3001 ttttttcagt catctattaa gcttctccat tatctgtatc gtacgataca gataatataa
     3061 accacacctt tgatagcggc aatctttttt gctggcaatt tcacgcccag ggcgggcatc
     3121 gagcgggtaa tattgctggc aaaaatcaac ggccaaaccg gctgcaggaa actggtttgc
     3181 tggcaagcgt gatttactgc tgggcttgac accttgccat tgtatgacgc ttgttttagt
     3241 gtcaaatgaa caaccagcgc gagaagaata taggaagcaa aagcaatgga tatcaaacct
     3301 aactcagtga tccacctgga gccaggcgaa acattacccg gctataccaa tctgaaaccc
     3361 gtacctgata agtatttcga tgagttaaag gcactcataa ataaactcaa tactcaatcc
     3421 ggtaatattt acctcaagtt aaaacagatg tacgcctttt tggatcgttt taacaaagaa
     3481 tttgtgagca cgtttacctc ctgtcaaaag gggtgctctt cgtgctgtaa aatggatgtt
     3541 catttaaccg cgcttgaggc aacacatatt gcacaagcaa gtaaacttac tgccagagac
     3601 aacccgctta cgaccgggca tgagtcaaaa tgtccgtttt tgtctgagaa gggaacatgc
     3661 tcaatctaca attaccgccc gctgttatgc aggacatatc atgtactcac tccgccggag
     3721 atgtgtaatg acctggatgc acaagtcatg cagtatggtt ctcaaagcgc gaacatggga
     3781 aatcacatct acaaaacaat agcagagtgg atctattttc agacgtatca ctgcactggt
     3841 aagttagaaa ccaaagatat cagagactac ttcccttatc cccgcgaaga tatccaaaga
     3901 tttcttcacc ataacccccc gcgccctttt tgctaaaaaa aagcctctga cgcagtgtca
     3961 gaggcttgtt agattactgt tcgtcaccag cagtgctgga agcatctttt ttgatgagaa
     4021 actcatcaag agaacggcca gcttttaacg cttcgtcgat tggttttggt gtccggccac
     4081 gaccagacca gtatttcgtt tcgccgtttt catcaaactg atatttaggc ggcgcttttg
     4141 gcaacgtctt tttgcgtgtt ttttgcgcat cttcggacag gccaagcagt tcttccggtg
     4201 aaaaaccttc tccggcaatc aactggagca gttcctggcg ctttctttcg cgctctgcgc
     4261 gttctgctgc ctcacgctct gcttcttcac gacgttcttc gatcacggcg ttaagctttt
     4321 cctgcatttc cagcagttgt tcaaagctgg tttctcgcgc aaaaacacga acagagcgaa
     4381 tattcaggag tagctttttt gttgcgtcaa acgtttcatt ttcactcatc gagttaatcc
     4441 ttatttttta ctgaactgaa acccaagttt acccgtagtt ttatcctgca aaacaattgc
     4501 agcgtcaaac tttttcccgg ttttattact ggtaaagcct ttaatctctc cggttttacc
     4561 ttttttgatt aatgtctcaa cctgattttg tgtcagtttt tttcctgaca cttcagacca
     4621 gattttaaac tcgcacccgg aacagaaaaa gcctttaggc cggataataa tttctttact
     4681 gcatgaaggg cagggggctt cgagccggga catggaacct tctgatctgg attttgcttc
     4741 catatccggt ttgccgtctt tgtccgggaa tgttgtcttg cattcagggt agcaggagca
     4801 tccccagaaa aagccatttt gccccttacg cttacgcagg aaaccgttgt tacaaaccgg
     4861 gcagggcgtg gcatttgaag aaatcgaaat tccgttcctg tccagctcgt cgattaaccc
     4921 ctggacgtac tcatcatttt ccttaataaa ctcctcaact gtcagctctc cggctttgat
     4981 ctgagactgc ttttcagccc acagagcgga gatatccggt ttagtgattt caggaggcag
     5041 tgcagcgcaa aactcctggc cttgttttgt ggttttccat accagctccg aatacccctt
     5101 ttctttttcg atggagataa gcccggtatt tgccgccagt ttctcaagaa tacccgcgcg
     5161 ggtggcttcc gttccgatac tgccacgatc actgctgccc tcgtctttcg cttccagcgc
     5221 ctttctgaga gccggatcat caatgaattt tgccgcactc gtcatggcgg ccagtaacgt
     5281 tgactctgtg aaatagcgag gtggtgtagt ctttttcttg tccacgtctg ccgattcaca
     5341 caggccgctg tcattaaatt tcagcaatga aaggtcaaaa ccgtccgttc cggcatcttc
     5401 ggtttcgtcc tcgtcatcac tatccttacc cagcgcttcc cagccctttt gtaccagaac
     5461 gctctgtgtg gccgtaaagg tatccccctt aatatcaaag tggattttcg tcttgttgcg
     5521 gatcgcatcc gggtagaaca gcccgataaa gtagacggac accaggttat aaacgttccg
     5581 ctctttttca ttaagctgaa taccggctcc gctttttgtc gtcgggatga tggcgtggtg
     5641 cgcctcaatc ttactggcat taaaggcttt gtgtttctgg cctttatcca tatctacgat
     5701 cgcagacgcc agttcgggta cggtggccgc tatagcagtg gcaatatctc cggcctggta
     5761 ataatgctca tctgaaagat agcgattgtc agaacgtgga taggtcagaa gcttgtgagt
     5821 ttcatacagc ccctgcatca catcgagcgt atcttttgca gaataaccaa atcgctttgc
     5881 gcagagctgc tgaagcgtgg aaaggttaag cggaagcgga ggcttgctat tttccggctt
     5941 tgtcgtcgcg atagtaacaa tagcatcctt acccgtaaca cgtccggcaa tatgcgcggc
     6001 ctgagcttcg gagatcaggc gttttttctc gtctatctga tcgtcgtctg ttgtctgata
     6061 tttcgcctta atctgatgcc cgttcatcga cagactggcg aatacatcgt aataaaagct
     6121 ttctgtgtga ttctggttcg ccagcgtgcg catgttcacc agaccaagaa cggcactttg
     6181 tacccggcca acgttcagaa cgccgtcata gcccttctca cggcctttta atgtacatcc
     6241 ccgcgtcatg ttatagccaa aaccctgatc gcacagggaa cgcgccaggg cgcttaacgt
     6301 cataccgcga aatttatcgt taggttgtaa attcgacagt gatttttgca ctggcgcaag
     6361 gttgaggtcg gcaacaagca gtcgctggac gggtttcttg tttccggcat aacccagaat
     6421 ctcatcaacc agcaaacatc cttcatcatc ggggtcgcct gaatgaacga tgctgtcggc
     6481 tttatcgata agagacaaaa taatgcgggt ctgtgcttct gattcaggct tgattttata
     6541 ttttggcgga taaaccgctt taatgggtaa atcactataa cgccagttgg aatatttgtc
     6601 gtcataatcc tgtgggtcgt aaagctccag catatgacca taacaccagg taacgacatc
     6661 agaaccgtgt tcaaaatagc cgtttttctt ttctgtattg ggatttccgc ccagtccttc
     6721 aaaaatagcc tttgctaaag acggtttttc tgcaataaaa agtctcatta atcaatcctc
     6781 aaggtaactt gaatagtcag gctgcgcacc attcttttta aagatttctt ccctttcctc
     6841 tatgtcttct tctgcctcct gcacactaag gtcaagggtt cctttcaaaa aggcacgcgc
     6901 attcttgatt acatctactg ttaattcgtt ttttgttccg cgaatcatgc caatagcaat
     6961 atttattatc tcctttattt cagcaggagt cgttctgtcc agcaccgcgc caagttcttt
     7021 aaggtcaata tttatatcgt ctggtaagta cattttggca caagcggcag cggctttagt
     7081 ccgaaaagca cctacagata tgattctgtc aattctgcca gggcgttttc tgatgcgcgg
     7141 atcaatccgg ttaggatagt tcgttgaaaa aataacatat gtcccggcaa gatttcgcgg
     7201 tgtatcagcc ccatcaagaa aacttaacgt tccggcatca gcccgataaa gttcatcaac
     7261 ctcttcagct attatgataa cgggtatttt cttttgagct gcggcatagc aaacatcttt
     7321 aaagtaatca tcagcataaa caaatacata tttatcctga tattttgccc ccaaagcttt
     7381 tgcgatagtc gttttccctg tgcccggagg accagtaaaa agcacttttc gcattcctga
     7441 ctgaccataa cgggtgtaga aatcaatatc cgaaaagaac tgattaaagt cattctccag
     7501 ctcattaaac tttggatgaa cagaaaaacg taaagcgttt tctatttctt ccttccttgc
     7561 cttaccgtga taataaacgc cgtcgtaaca cccgattttc cagatacctt tacggggtaa
     7621 agagcggttt aatttagcgc gtccagcttc gcaaagatta tgaagcatcg acatactttt
     7681 ggttgtgcca actgcgtact ccattctgac tttagagtta agaatgaagc gaataatatg
     7741 aaccacttca ttttgaccaa gatgaatccg gtatgctccc gaaccatctt taaacataag
     7801 ctttttaatc ctcccggctt tagccataac agggctactt cccgtatgaa cgacggttga
     7861 agcaacgggt tcgatatcaa gattcgaata aattttacga ttctttctgt ttataaaaat
     7921 ctctgccact ttaggatagt aatcacaatc ctttacgaca gtaccaaaat tatcttcacc
     7981 gtaatattct ttatggagcc gtaaagcctg ctctaccaga gttaatttac ccatatgcgc
     8041 accatgtata ttaaaaaccg tgacgttgcc tgaagatttt cttcaggcag aggaggggtt
     8101 ggacaagcga agcgcgtcag ttttgtttat gacgccactc ccacggagga acgggattac
     8161 tgtcagccca caggccgcgc ttctgtgtct gagcttcccg ttgcagagcc ggtaatgagt
     8221 tatcggtgtt gtacctgtca taaacccatg cagccccttt tagcacttgc tggcggttag
     8281 cctcagtgcc gttcgctgtc accactcgtc ccaggactcg cccgtagcga tctgtttgcg
     8341 tataagagac ggtaactgat tgccctgcca gcagggcttt aagttgattg gttgaccagc
     8401 gaccaaaggg ttgttttttt tccggtgcat cgatatttat caatcggact cgaatcggaa
     8461 cttcatacac aacaattttc gcgggtagtg ttttaacttc gatggtatct ccatcgagaa
     8521 cgcgaataac tttcccctga atctgagcag cacacaatgt agctggcaac agggcaacag
     8581 caaaggaaag aacattaaaa aaatgtctca ttgctttctt ccctcccata ttgctgagag
     8641 tcttttaaaa atggataaaa cgacgactaa agccactgcg ataacagcca acgaaggaag
     8701 tacatagctg tataactcaa ccatgaaatt aataaactca atgagcgatt gcggctttaa
     8761 ggaagcatcc cacaaaacga taacaaaacg ataaagccca tagacaattg ccggaaacca
     8821 gaccataaac aacaaaattt cagtaaaaac agaagcgacc gatttgacac tatcgataat
     8881 ttcaattccg gcaattctca cacttcttgc atcaggtacg ttaataattt tcatcctccc
     8941 acccctgtaa aaaatgttcg caagttccag tctcaaacct gaaacttgcg tattcacatc
     9001 agaattaaat caaacagaat taaaagtttt tcaatcggcc aaagcttgaa ataattttac
     9061 ttactgtacc tgcatcagcc gcaggacatt catttagttt tttctttctg gcatctgccg
     9121 tgtgatcggg taaaaaacca tgtttattct ttttcctgat tgacataaaa cttttataca
     9181 tcgggtcaca ttctgccgga ctacttccgt caagttttcc ggcaaggcat aacaccattg
     9241 cacatggatc gttcgggtcg gccttttcct cagcatgagc agttccggca gcgaacgcac
     9301 aaagcagcac tgccgtggtt acggtctttt tcatatacac ctcatggata acgggcaact
     9361 aacgccctgc ggttgttcaa ccccggctca ataaaattac aagaatttta ttaatccggc
     9421 gttatcgtga taaataaaca ctggtagtcc ggcaagaaaa acagttcatt ttataacgat
     9481 actgtatgac accgtttgca ccgttcgccc tcaaccagtt tttagctgtg aaaaagtctg
     9541 ttccgttact ggtttcaaca atcactgaat cagaatgtgc aacagaataa aaaacagcat
     9601 catcgtaaag gcggttttca ggcctgaaat tatggataac cttaacccac gatgaattaa
     9661 ccttgtctga tttaataact gagtatgtcg gttgccagtc cattaattgt gtattcaccg
     9721 tttcaggatt ctgttcccag tcaacctgtg gtggttcagg cggagaggaa caggcagaga
     9781 gaaaaaggac actgaccatt aacatttttt tcatagtaaa acctctgact ccagctcacc
     9841 gacggagagc attttttctt tagaaggata tttaagccct ttaacaccca atactttttt
     9901 agtcttattc agagaggcgg ttaactcggt aagtttgggg cttaccatca tcagcctgtc
     9961 cataaaatac gggtcgagga agtagagggc tttctcagct ttaaccgggt tttcaccttt
    10021 aagaatgata aattcttctt taaaatccag cgttcccagc tcctgcggga gcacaagggc
    10081 gcgctgcgcc tcactttctg aactgctttt cgacgttgag cgtccgcttg tcttgcttga
    10141 acctgtcgat ttggtggtga tatagcccag cttttccgaa atctttttag cgtcatcttc
    10201 ttcactgaca gcgtagataa tccggcaggg atgcgcactc atcagcgttt tggcaccttc
    10261 aacaccatag atttcattaa gctgactgat attctggtaa atcgtcagaa gtttgagttt
    10321 aaacccggca atataacccg aacctttttt aatgattggc atgtaaccaa tagacggaaa
    10381 ctcatccagg aacatcagac agtcatgttt cagtgtcggg tcaaagtcag ggttttctcg
    10441 taacgtgact tccacaacga agttaaagaa caggttgagg aagtcatagg ccagggacat
    10501 atcttcagca ttaacgccaa cataaacagt gatatcttcc cggcgcaact ggcgaaggtc
    10561 gaaatcattg ccgtctgtac atttgcgaac agtcggaaga taaaacagac tcattttctt
    10621 acgaaacgaa ccatcaatac tcgaacgttg ttcgtcttcg gtttcgtgat actccctgat
    10681 tttggtcagg gcatcgcgca gatgatacag cgcgttttca tcaagtcctt ttgttccctc
    10741 caggtcttct cgcttactga gtatcagctc ccggtcgatg ttgctgtaca aatcgactac
    10801 agaaccaatt gagaaaacag gtttaagccc gaactcacca agccaggagg ggtcgtagtt
    10861 aataaagaaa tgaagcaatt ttgccagccc cgtccagtac tgccccgcga ggttattaaa
    10921 gtgtgcttcc gctccggtca gaccgtaaga cgggaacagg atctcaatca gcttcagcag
    10981 gtctttagcc ccgctctctt cttttaaatc aatatagaaa agcgggttaa actgatgcgt
    11041 tttactgttg aaagggtcaa gaagatagac cttattaccc agcagttttt cacgtacctt
    11101 actggtgatt ttccacagtt cctgttttgg atctaatgca atcagggaat gtttcctgac
    11161 cagcaggttt ggaataccta ttgcagcccc tttacctgcg cgggttcctg cgccaagtga
    11221 tacaaaatct ggtgcggtat accacaggta tttaccttta tatgcaccta caagaatatc
    11281 agtatcgttt tctttctccc atttcagaag tttcgatttt cttaaatcat tatcactggc
    11341 aaacttcgcg tcaccataaa gagcaacatc cgttttattc agttgccaga taataaaaac
    11401 aggcacaatg agagacgaaa ccatacctga taaaagagag gggattgcgg ttaacctgat
    11461 atctgtccgt atattactct ctgttattat ccgccacagc agcatggaat caaaattctt
    11521 ccatatataa agcggcgttt ttccgttcag gaagtaaaca gcaactgagc cagtataata
    11581 cgtcacaaga cacataacca gaccgataaa agcccactgg cctttatcgg gtagtttcag
    11641 agacataatc actccttcag cttttcaaag tattcctgaa aatgaatatc acgatagtaa
    11701 atacccgatg caaatcggtt atcttcctca tcgatgtact taatactcat aatcacatcg
    11761 acgttgctta acaccctccg caatataatg ttataaggca aattctgaca ttcagggttc
    11821 atatagcacc gttgaaccag tccaagcaca gcatcttcag gggtgctctc atggacggtt
    11881 gtaaggttcc ctgcatggcc ggaacttgag cctttgagaa aatcccaggc ttccgcgccg
    11941 cgaacctcag tcatgaggat tctgtccggg ttcatacgaa aaccagaacg taaaagagaa
    12001 gcggaactga cagtgctact ttccccttct gacgggtaat acaatttcac atgatttttg
    12061 tggaacctga actttgcttc atctgtatct tcaattgaaa tacatcgtaa atgatggggt
    12121 atgtactgaa gacaggcatt tgcaaaggtg gttttacccg cccccgttcc ggcacaaaat
    12181 accatcgttt tacccttaac caggcattca gggacaaatt gttcaaaaca attatcttta
    12241 aacattgaag ataattcatc ttctttatcc ctgaatttcc ccccttgatt tagccttgag
    12301 taaaaccctt gtttaacaaa ggtatcatgg tcgataaaca cactggacgg cttacggatt
    12361 gtaatagaca ccgtttcccg ttccgttgcc gggggaataa ctatttgtac acgctcccct
    12421 ccggggagcg taacagaacg taagggatat tctggcgtaa ccgacccacc atcatgataa
    12481 tcagaaattg cccctgcaaa tgacatacaa tctttaaaat ccatttttaa ttcatgctgc
    12541 tgccatttcc cactcacttt aacaaacagt tcatcaggcc gatttaccgc gatttcagtt
    12601 aaaccatcga cttcattaag ccagtggtgg aagtattcat ctattatttc ataaacaata
    12661 tgtctgttct ctgcatcctt cataattcac cttttccttt aagatcttca ataaaacgca
    12721 tgacttcttc gtaattaaat cgatgcgttt tgatttgttt cagtggtttg gggaaaggaa
    12781 cgccatttat tggtatgccc ttacgaatat gatggtatat ttcgctttcc cttattccta
    12841 acagcattga aaactctttg atatccagcg ttatcaaatt acctccgggt acccaccatt
    12901 tttaatttgt agatgctgga gaaatccaaa tcctgaccaa cgatcagagt aatgatttcc
    12961 ccctggtttt tataaagcgt tggagggata tttacgctgt ttgcaaacgc ttcgcgtgct
    13021 atatcagcaa aagcctggcg actgttagct gtatagtcgg tctgattgtc tttattactt
    13081 ttagccgctc cacttgcggc ctgtgccaca tcggggatca tgccaaccat catcgcgccc
    13141 ataaagcgat ctgcaaaatg tgtgtcaatc cagcctgaag ccccggcctc acccagcgct
    13201 cccgccgcct gtgtatcaat cagcggaata tcaatgaaag gttccttacg tgttctaagc
    13261 ttcgtagcag cgataaacac ggctccctga ccatgattga acgtgcccgt tttatacatc
    13321 agatatgcgg ctgttccctt ttcaattagc tttacattcc tgtttgcact gtatacgtcc
    13381 tcattaatcg tacagaccag cttgccagcc agatcagaaa taaatcgtct gtccagcgag
    13441 cacttaatcg cggtattttc aggaatgaaa aggttagggt cataaggaac gcgcgtaatt
    13501 ttagaaagct gcgcgggttg ccctgcatta acaggctgaa cctctgcctg tttgtcactg
    13561 ccgtccccct cgttcctggt attgctggcc gttgatgagc taccaccgga atttgaggaa
    13621 gcagagctgc tccccccata ggccacatcc agcgcacgac tgaagttttg ttgtactttt
    13681 tccggtggcg tttccgcttt gccgctgctt tccttccgtt cttccgtctt agccggttct
    13741 tccctggtgt tgaatggatc aacatcatcg ctgagtcctg tcgcgcgttt tactgaacca
    13801 gtagataccg cattactggc aggcttatca tcaccgctgg acattgcacg aataatcgac
    13861 gggatatagt agacagacac cccggcggcc agcagaagga aagcggccac aataatcacc
    13921 agccccttac gcgatttttt aagcttctcg atacccaggc gttttactga tttaccgtca
    13981 tcttcatctc ccttatccgt tttatcgttt tgctcaattt ctttctgatg ccgttcccgt
    14041 agtgcctgtt caatttctac agcggtcggg attttatttt cctgttcgct cactggatca
    14101 cctctttctc aacgttcggg gaaacggtat ctcctgccgg aagacggact ttgccaaagc
    14161 cccggttttc aaccccaaca acctgatttc ccatacgcaa aacaaagcgc tggtctacag
    14221 gaacaccaac aacggtgaaa ctaccgctgt ttttgactac cgggttagtc agcgtttcct
    14281 gttcgccgct cataacaaag acgctgggga ttttcttcag ggggctgaag ccaaaccagg
    14341 tatagcggcc atcgtcatac gcataatccg ggctgattga ctggctgccg tcagctaccc
    14401 gcttccagta atcccagttc ttgggcgact tcgcattttc aagagaacgg ttgattgcct
    14461 gctcctcctg ccgcttgtta agagcctcaa tccgtttctt ctcgatttcg gcttgctcct
    14521 tccggcgttc ctgcgggtac tggtaattaa caacaaacgc gatcttatcc ggctgccgga
    14581 acgttcgggc gttcagctcc atgctgtaat tacgttttgt tgtgcgaaca aacaggtttg
    14641 ttcgccagcg ttccaggtcg ttttcaggat caagggctac actgaccacc tctgaagact
    14701 ggttgccatt gtcatcggta ctgttcttct gtacggtctg tttgacgggc ttcacttcga
    14761 gatacaccag gttatcttct ttgttcactt cccagccctg gggaaagccg gttctggcac
    14821 tgatgaccgt ttcatcctcg tcaaatacca gtgtggttgt atagccaacc gcactgttta
    14881 tgactgtcgt gttttgcggg ttataactga caatctgatt acggggatca aaacggctgg
    14941 ctgctggcgt aacggctgca tggcagacgt gcgacaccaa cagaagcgcc acaattagcg
    15001 ctcctttatt cattttcata gttttcaccc tttcacttct ttatcgcgta catagctcgt
    15061 tacaacgaat ttaagcgggt tattgtttcg gtcacccgat gccatttcga cctgtggttc
    15121 aatacggtag gtcaaccgga cgttccagta ttcataatca tcctggcctg tcgcgacatt
    15181 tcgaatggtt aatttaaacc ggatatatgc tcccatatcc ttatcatcgc gccgggatga
    15241 cggactgata atcacggacg gattatcctg aacagatgcg gttttttccg ctttattata
    15301 aataaccttt ggtgactggt tgccgttgat taaagcgttg taatccgtcg ccacattctc
    15361 agcgctgtat aacagaacat aatcataatc atgctggaga ctgaaatagt tatacccttc
    15421 acgacgcttc acatactgag taatgaagta tttcgccagc gcttcatttt cagagaggtt
    15481 ttctttttta acagaagtca gtctttcagc aacgccagtg acaccattaa taagataagc
    15541 ttcgacaacc gtactcttta acggtagcgc tccattcagt gccagcagag acaacacacc
    15601 tacggcaatt cctgcaatac caccgaattt ataaatgcgg ttcattttct cctgcatcaa
    15661 tacatttttc tcttcaaaag tagttgaaga gtctgttact tcttttttct tgttaaaacc
    15721 gggaagtttt aatcctgaga gtttaaaccc cattgcgcat tacctctgct gaattaacag
    15781 gttcgctttt tccggatacc ggaggttgtt tatgtgacgc ctggcatccg acaaggcaaa
    15841 gcgtcacgat agccgtgata agtagtttca taatgtgaga acctaaatag aggagtgaat
    15901 atttactgcg gaatactcag agttgacaga tttaaataat aaatcggtta acctccatat
    15961 ttctttttcg ccgcatctat cgcagcctgg cgaagcctct tagcgccaat attaacgccc
    16021 tgcccggtca gattccccaa cttaccggat acaccaggag actggttgaa ccctccatcc
    16081 tgacggcgaa cgcctttcca tgcgccaatc cctgcatttt tacccatacc aagcagatta
    16141 cgccccatac gggaagcgcc gaacacgcca gcaccaatcc ccattgcggc tgccccttgc
    16201 atcgcgcctt caacacctac ccctgcgagc tgggaagcat aggttttggc ttgccagata
    16261 atccaggcca taaatgcacc tgcaaccccg gcctgagcac ctgttgatat cagatttaat
    16321 tcatctgcgt tagctatcga gattgtcaga atgccattaa ggaacgtcat tcccgcttta
    16381 attgccagtc cataaaacaa gaatatgaag catgagctaa atattaactg aagccagcta
    16441 ttaaacatct gccttaaaaa accaaatgaa agacaaataa taaacaacgg cgcggtaata
    16501 gtaagtatct taatagtaac ttcggcggcg agataaacta ttgaagttag caataatgca
    16561 attatccctc cagcatatgt taataaagat gcgattgccc catctgtttt cacatacttc
    16621 gaagtatctt tggacataag atatgcagct acctgctgaa ctttctccca aagctcgtca
    16681 acccatttcc aaggatcacc tcctgcaaat gtttccttca gcccatcaat ggcctgagtt
    16741 gcactatcta accacccccc catatttttg acaaaaacaa gaattaacca gaatcgacat
    16801 agattccata aaaaatcctg cactgcggtt ttttgcttcc caactattgt ggtataagca
    16861 taccacaata tataaatgga aataccatac ttacccaact gagacgctat tgaagaaaag
    16921 ttatctaact gaccagtcaa agactggtca agagttttaa caataacgtc atgagcggcc
    16981 tggaaatacc tagaagttgc catagtggct ccttttaact tccatcttta cccaatacat
    17041 ttttgaatgc atctttatag tcaacatgct taggatgtaa ttgcttatac ctaaacaaac
    17101 cttccttagc gtttttacaa ttttcgctat cactcccaga ctttttgcat tcagctactt
    17161 ttttcgttgc atcgtctacg tgagactgcc accagtctac agatttaacc tcttcacacc
    17221 cggtaagaaa gaatgtaagc cccaacgccg caactaatcc atattttttc aaaatgcccc
    17281 ctcagttcat ttcataactc tgctgctcaa atgctgcctt ataatcaaca actggagttt
    17341 ctttattaac agcccctaat tgtttctcct ggaaagcagc ctgtaacatt ttctccttgt
    17401 attctgcaag atcgcgctgc ttatcgcgat acatttcata ctgaaatttg agcttgttaa
    17461 atttcaggct tgccagagat acagcgtttg tcgcgtcctg cgtggctttc gtgtcctggg
    17521 cgttcttcac ctggtcgata aggtcggcca tttcctgatt atcttcacgc agtttacttt
    17581 ccagcttatt gccgtactcg atccccgcca gttgggacag gaacgaggct tcacagcccc
    17641 gcaccaggtc gcccgtatat cccagcccct tacaggtgtt tgtcacctta taatcggcca
    17701 gtagtgatct ggcttgctca gacagggtgg cttccgggtt cttaatgtaa tcatcaatga
    17761 aggagttccc ctggtcgtaa atattctcca gctccttact gacagattgc gcagactgca
    17821 ccagtccctg aacatcacga atgcccgttt tggtcagcag ctcctgctta taggcattaa
    17881 tctgtttctg gtagtggttc acggtgtcct gccactgttt gaggcgttct gtccagcgct
    17941 gcgcttcgac agcccattca gggtttgcat caatggcaac gggaacacca gcctgagcac
    18001 cagaaataac cgtaacagag gcgataagtg ccgcaacgat atgctttttc atagggattc
    18061 cttaaatcag gtttgcacgt tccagatagg tatcaagcca ctcttccggc ttcataccct
    18121 ctttccagat ttcatcgaac agttcaagct ggggtttcga tgcgctgaga accggtaggt
    18181 aatacgccgc ttttcccaaa tccagtcttg cgaaagccgc aaaatcatca ctttccccct
    18241 tcctctgtgg gtttttgacg accaggaact gacgagataa cgggtcaatg gctttaattt
    18301 tgtcaaaata aagttctctc acttccaact cgtctacgta ctcactacgt ttcgctttgg
    18361 ggttcgccag ataaatatgt gttgcgcact gctcacgaac ggcagccgcg ataggtgatt
    18421 taattagttc atccggcgat tgtgtcgcta cgacaagcac catatccagc ttacgcgcgg
    18481 ttttgagttt gttataaaca aaatctctga acgcttcgtt gttaatccat tgccagaact
    18541 catccatgta gataaggagt cggcggccat cagccagcat ggtgacacgg tagatgagat
    18601 agaatgaagc ggcgctggaa agtactttgt tatccagaaa ctccgttccg tctataccga
    18661 aaacgtccag gttgtccaca tcaaacgtat cgtccgggtt atcaaacacc cagccaaatt
    18721 cccctccctg cacccaggca ttcaggcgtg ctttaagccc atgccgccgg gtttcagtat
    18781 catcaggctc cagaatgagt ggtcgcaact tactgatcgg ataagagcgg tttgacctct
    18841 gcatcaggcg ctcaacggca tcagaaatca ggcggttctg gtaatcatca agcggctcac
    18901 tgttaagcgt acaaagtagc ctcaccaggt ctttcataaa ggaaatattt cgtttggttg
    18961 gtggcaaagc cgcagggttc cagccagtcg gctctccttc tttcacccga aagtaagcac
    19021 ctcccattga acgtataccg ggttccgctg cccgatcttt gtcgaaaaat actgtagtta
    19081 acttctttaa tttcctctca ggtggaaaag actccggcgt tccgaactgc tgggcggcga
    19141 aagccttagt catcatcact accgttttac ccacgtttga cgtaccaaga atttcggtat
    19201 gccccagcgt ggggttttta ccgaagtaat tttgatgctc ggtagtcatg tgatagttca
    19261 ggttataaat atcatttcct gagccgccca gggtgataag gttgttaccc caggtatttc
    19321 ctttctcctt gcccgtaaag aaattatgga agctttccat ttcggcaaag ttcagactgc
    19381 tgatagaact caggcgtggc cgtaacgtgt agttaccagg taactgagca aaatacgctg
    19441 cgccaagact caaggttgaa taggttacga tcagccctaa atcctccagg gtcgtcgtta
    19501 caatattggt gtccttcacc agacgctccg gcgaatctgc gtaaacaatc agtgtctggt
    19561 gacttttccc gaaagaaata tagccgctgg agaccatatc gagaccaact ttcaaatcag
    19621 cgagcaagga tttagcggca tcatcggtca tttcgagttt gtcgatctga tcatccagcg
    19681 ccttgattgc agactgcttg tctatcggag ttaaagagga agtctgtaca tactcaaccg
    19741 ggagatacat cagtgcatca aaaattccgg catccgtttc ctgaaagtag tccttgatct
    19801 cgatgcaacg aaagtaccgg gcatggtcag aggcagtgat ttgtccagca tcgttaccaa
    19861 agaacaggtc tttaccaccc aggtatgtat aaaacggact attggtgact ctgactttct
    19921 gccagcgccc ggatagcagt ttctggaaca gcgtaagctg ttctgaataa actatattat
    19981 tttcttcata taacccaaga cgacgggaat gaaaccggga gagataggtg ctcaatctgc
    20041 cacatatttc attcatatca ttaatgggtt cttcgaagat atttttatta ccctttttcc
    20101 ttgaaagaaa atgcgtcact ttatcttcag cgctgaacgg tttatagcat acggttaagt
    20161 ataatttgtt ttcaaaatac tcagccgccg aaagcgattc gtaataatcg ttcatcactc
    20221 tgttgacgaa tgggatcttg ctgtcaaata caggtcgcac ttcttttcta acccgaatac
    20281 gatgcgtata aaaggtaacg ggtttcccat caaaactacg tatcagcgta ttgagctggt
    20341 cagtaagcaa ggctagatcc gcttcatcta cacattcaaa ataagctccg tcaatctgcc
    20401 aggtcgccag tagatcatgg tttctggtaa caatgaggtt atcagtgatg tgtgatgaat
    20461 acgggattaa ctcttcaaga gaagactggt ctttaagctt cataaaattg tccactttcg
    20521 atatatcgac tgcgtcgtac gaactccccg cataatggac ggcattgaag cgcttgtttg
    20581 ccagaggatt acctttgatt tgcattcgca gataaagcag gtcaaaaaag cgttcatcta
    20641 tcttcgtaac tcgcttgatg gcataccagc cagggaggat gagggcatac atagcctcat
    20701 gaatccagac accaagcaaa acgcagataa cgcacattcc aagaaagggg tagagcggaa
    20761 cgcccagccc cctaatcaaa gcggggcgtg tcagcccttt aaaaacggta ctcattttta
    20821 cgcccccttt tattacatat tacgctaacc agtcaggcag atatggcgca acaataaaga
    20881 tgataatccc gataattaaa ggcgtaatcc acgctgcatg ctggcgtgag aagatagcga
    20941 tataaccaat taccagcgca atgatggtga ttgctggctt acggatatcc gtcagaaaat
    21001 cgataatctt ctgcaagaag ccagttgttt tagtggatac atcatcagca aaggcgggtt
    21061 ctgctacaca aagcatcaat gcagccataa atacagaaag agttaaataa cgtttattga
    21121 gttttaacat cgtttgtttc cttttcatct aaaacagtgc cacgcatggc atattgggga
    21181 tatacggtaa cttctttagg tttgatactc tgtgttacat tctcctttct gattgcatct
    21241 ttaactgaag gaaccaccca gttttttttg ttatcaggcg gattaaaccc tatgcgctgt
    21301 atataactgg tgttattaaa atctgattca ggtttagttc ctgtttctgg attgccggaa
    21361 taatagcaac tcaggccacg taaaatatcg ccaccacgtt tataacaatc caccaaaatt
    21421 tgctctgaaa ccttgagatt ttcgcagggg tcaaacattt tctcagcggt tgtattgaag
    21481 tttgcaaaat tcgtactcgt tatttgcata agaccgactg agtaacgatg atttcgtgat
    21541 tcaatttgat ttacgatctg gagtgcagcc tccttagttt tgggaaagta ggacactacg
    21601 cctttgtcac cgggcttacg ttcaaccttc ggtattatct cggcaatagc atatgggtta
    21661 aaaccagatt caacccttgc aacttcatgc gcagtatcgg gatgaacgct ggccgcgcat
    21721 tgcaaagcaa gcgccgcaaa agcagtagta gataacatga ttttctcccg gccagaacag
    21781 cgcggaaagt ttagactgaa tggttagtca tcgaagtcgt catcgtcttc atcgtcttcg
    21841 tttatcagct cctcaatagc ctggctggaa tattttttta tgaggtactg gacaacacca
    21901 gagcttgaaa tacgtcgccc cctgcgatag ccaatctcca gagcaatttt atccagttcc
    21961 tcacgagaag ctgagggcag gtagagtgta ttacgttggg gcttgcgtct cgttgcggtt
    22021 ttttttcggg tagcagtcat ttttcttccg tttcattttg tgggttaagc ttctctctgt
    22081 gatgtagtgg atgaaagcca tacttctttg ctgcggatta tccatgagta gaatttcagc
    22141 aaaatcgtgt ggaattgatt tccttaaaag atttgcctga gctgttgtat gtgattgtct
    22201 gtacaaaagc tccgccatca catcttctgg tacaggtaaa ggctcaccgc caaatgagat
    22261 aaagcgttgt acgtaaggga tagcagttat tgtagtggaa tgaacctctt caaaatctgc
    22321 ctggataaaa agataaccgg gaaaaagcgg caaacgtctt tctcgatatg aaattatctt
    22381 atccgggcga cgataccttt cagtctccat aggacagaag taagagaagt tcaggcgttc
    22441 gagatgagca aaaactgctg caaatctcgt agtggtgaat tgcaaaacat accattccat
    22501 agtttttcct ttcaggcgct ctctcgactc ggatgacccc gaatcagaga aaaaaaacaa
    22561 aaggacaaat ttacatctca taaaaacttt atcagctaca tgtgctgcgt tcaaccacac
    22621 aagcagatct ttagatcgca tttacgacac atttcatttt gtttgtgctt gtattttatt
    22681 tatatctaga tacatatgct atttgataac aaaa
//
LOCUS       DACBVI010000065        22155 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.065, whole genome shotgun sequence.
ACCESSION   DACBVI010000065 DACBVI010000000
VERSION     DACBVI010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.065"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            179..1548
                     /locus_tag="IU325_12255"
     CDS             join(179..659,659..1548)
                     /locus_tag="IU325_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HAP5809795.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLK
                     VYEERGEAGLRALKIGTKRNIAISVDPEKAASALELSKDRRIEDLERQVRFLETRLMY
                     PKKAESLSSSHEKVKVLNELRQFYPLDELLRAAEIPRSTFYYHLKALSKPDKYADVKK
                     RIGEIYHENRGRYGYRRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKRYRSYRGE
                     VGQTAPNVLQRDFKATRPNEKWVTDVTEFVVNGRKLYLSPVIDLFNNEVISYSLSERP
                     VMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLD
                     NAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQ
                     TYMPRV"
     gene            1650..1802
                     /gene="hokA"
                     /locus_tag="IU325_12260"
     CDS             1650..1802
                     /gene="hokA"
                     /locus_tag="IU325_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HAP5809796.1"
                     /translation="MPQKYRLLSLIVICCTLLFFTWMISDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(1990..2202)
                     /gene="cspA"
                     /locus_tag="IU325_12265"
     CDS             complement(1990..2202)
                     /gene="cspA"
                     /locus_tag="IU325_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HAP5809797.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(2483..2773)
                     /locus_tag="IU325_12270"
     CDS             complement(2483..2773)
                     /locus_tag="IU325_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HAP5809798.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            3207..3917
                     /locus_tag="IU325_12275"
     CDS             3207..3917
                     /locus_tag="IU325_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 family protein"
                     /protein_id="HAP5809799.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(3967..4941)
                     /gene="ghrB"
                     /locus_tag="IU325_12280"
     CDS             complement(3967..4941)
                     /gene="ghrB"
                     /locus_tag="IU325_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HAP5809800.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILVMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETYHLF
                     GAEQFGKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(5045..5704)
                     /locus_tag="IU325_12285"
     CDS             complement(5045..5704)
                     /locus_tag="IU325_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HAP5809801.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            5857..8190
                     /locus_tag="IU325_12290"
     CDS             5857..8190
                     /locus_tag="IU325_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAP5809802.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVLSSRGALTTGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPEKPQGIRGQDEFVRVSWDEALELIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAGICGVNAEKIRELAALFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGTDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELSPFAEFWQANQL
                     IEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYGYQDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDIVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(8159..8599)
                     /locus_tag="IU325_12295"
     CDS             complement(8159..8599)
                     /locus_tag="IU325_12295"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP5809803.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYHAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(8596..9159)
                     /gene="tag"
                     /locus_tag="IU325_12300"
     CDS             complement(8596..9159)
                     /gene="tag"
                     /locus_tag="IU325_12300"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP5809804.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRASFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            9317..10015
                     /locus_tag="IU325_12305"
     CDS             9317..10015
                     /locus_tag="IU325_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HAP5809805.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLADPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            10244..11452
                     /locus_tag="IU325_12310"
     CDS             10244..11452
                     /locus_tag="IU325_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate antiport family MFS transporter"
                     /protein_id="HAP5809806.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            11776..13467
                     /gene="eptB"
                     /locus_tag="IU325_12315"
     CDS             11776..13467
                     /gene="eptB"
                     /locus_tag="IU325_12315"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HAP5809807.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVREGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAREAAAN"
     gene            13559..13635
                     /locus_tag="IU325_12320"
     tRNA            13559..13635
                     /locus_tag="IU325_12320"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13593..13595,aa:Pro,seq:cgg)
     gene            13670..13803
                     /locus_tag="IU325_12325"
     ncRNA           13670..13803
                     /ncRNA_class="other"
                     /locus_tag="IU325_12325"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            13836..13971
                     /locus_tag="IU325_12330"
     ncRNA           13836..13971
                     /ncRNA_class="other"
                     /locus_tag="IU325_12330"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            14600..16207
                     /gene="dppA"
                     /locus_tag="IU325_12335"
     CDS             14600..16207
                     /gene="dppA"
                     /locus_tag="IU325_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HAP5809808.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVAA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            16358..17377
                     /gene="dppB"
                     /locus_tag="IU325_12340"
     CDS             16358..17377
                     /gene="dppB"
                     /locus_tag="IU325_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HAP5809809.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            17387..18289
                     /gene="dppC"
                     /locus_tag="IU325_12345"
     CDS             17387..18289
                     /gene="dppC"
                     /locus_tag="IU325_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HAP5809810.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            18300..19283
                     /gene="dppD"
                     /locus_tag="IU325_12350"
     CDS             18300..19283
                     /gene="dppD"
                     /locus_tag="IU325_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HAP5809811.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLSDGRQSKCHYPLDDAGRPTL"
     gene            19280..20284
                     /gene="dppF"
                     /locus_tag="IU325_12355"
     CDS             19280..20284
                     /gene="dppF"
                     /locus_tag="IU325_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HAP5809812.1"
                     /translation="MSTHEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIEMPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(20314..21585)
                     /locus_tag="IU325_12360"
     CDS             complement(20314..21585)
                     /locus_tag="IU325_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAP5809813.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFTNITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            22061..>22155
                     /locus_tag="IU325_12365"
     CDS             22061..>22155
                     /locus_tag="IU325_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HAP5809814.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWL"
BASE COUNT         5411 a         5410 c         5811 g         5523 t
ORIGIN      
        1 tttttaggat tatatcaagg agaagaaacg aacttattaa gctagaatag ccacgggtgc
       61 ttgagactgt ttgtctcagg tattcaccga aaggcagaca gagaaaagcc ccacctgact
      121 ataaatcaaa gtgtactgca cccattttgt tggacgatga aatggaatag cccctaatat
      181 gtcaaagcca aaataccctt ttgaaaagcg ccttgaagtc gtgaatcact acttcacaac
      241 tgatgatggt tacaggatca tctcggcacg ttttggtgtc ccccgaaccc aggtcaggac
      301 atgggttgcc ctctatgaaa aacatggaga aaaaggttta attcccaaac ctaaaggcgt
      361 tagtgctgat ccagagttgc gtattaaggt cgtgaaagct gtgatcgagc agcacatgtc
      421 ccttaatcag gctgctgctc actttatgct tgctggtagt ggttctgtag ccaggtggct
      481 gaaggtctat gaagagcgcg gagaagctgg tttacgcgcg ctcaagattg gcaccaaaag
      541 aaacattgca atatcagttg atccagaaaa agcggcatca gcattggagc tgtcaaaaga
      601 ccgacgcatt gaggatcttg aaaggcaagt tcgatttctt gaaacgcggc ttatgtatct
      661 aaaaaagctg aaagccttag ctcatcccac gaaaaagtaa aagtactcaa cgagctaagg
      721 cagttttatc ctcttgatga gcttctcagg gctgcggaga taccgcgcag tacgttttat
      781 taccatctaa aggctctcag caagcctgac aagtatgcgg acgttaaaaa gcgtattggt
      841 gagatttatc acgagaatag aggccgatac ggataccgta gggtaacgct gtctcttcat
      901 cgagaaggga aacagattaa ccataaagct gttcagcgcc tgatgggaac cctctcactt
      961 aaagcagcga ttaaggtcaa gcgataccgc tcttacagag gagaggtagg gcaaaccgcc
     1021 cctaatgttc tccaaagaga tttcaaggct acgcggccaa acgagaagtg ggttaccgat
     1081 gttactgaat ttgtagtcaa tgggcgcaag ctgtatttgt ctccagtaat agatctcttc
     1141 aacaacgaag ttatttctta cagcctttcg gaaagaccag tgatgaacat ggttgagaat
     1201 atgctcgatc aggcattcaa aaagcttaat cctcacgagc atcctgttct gcactctgac
     1261 cagggatggc agtatcgtat gagaagatat caaaatatcc ttaaagaaca tggtattaaa
     1321 caaagcatgt ccagaaaagg caattgtctg gataatgctg tggtggagtg tttctttgga
     1381 accttaaagt cggagtgttt ttatcttgat gagttcagta atataagcga actgaaggat
     1441 gctgttacgg aatatattga atactacaac agcagaagaa ttagcctgaa attaaaaggt
     1501 ctgactccaa ttgaatatcg gaatcagacc tatatgcctc gtgtttaact gtccaacttt
     1561 ttggggtcag tacaaagtga ggcccctcta tgcatagaca ccataaggtt agcctcttac
     1621 tcgttggaaa tcaacacagg gggctgggaa tgccgcagaa atatagatta ctttctttaa
     1681 tagtgatttg ttgcacgctt ttatttttca cctggatgat aagtgattca ctgtgtgaat
     1741 tgcatattaa acaggggagt tatgagctgg cggcattttt agcctgcaat ttgaaagagt
     1801 aagagtcttc ggcgggaagt tattcccgcc tttcttacgg cgttgcgcat tctcattgca
     1861 cccaaattta tttttcacaa aaataataat agattttatt acgcgatcga ttatttattt
     1921 cctgaaaaac aaataaaaaa atccccgcca aatggcaggg atcttagatt ctgtgctttt
     1981 aagcagagat tacaggctgg ttacgttacc agctgccggg cctttagcgc cgctttcgat
     2041 ggtgaaggac actttctgac cttcgtccag agatttgtaa ccatcgttct ggatagcaga
     2101 gaagtgtacg aacacgtctt tagagccatc gtcaggagtg atgaagccga agcctttgtc
     2161 agcgttgaac cattttacga taccagtcat tttaccggac atagtgtatt acctttaata
     2221 attaagtgtg cctttcggcg atatggcgtg ctttacagat tttgaagcgt taaaggaatg
     2281 tgcactacga ggggtatcaa cgataactct tgaagggact tgccttacta cactgcttta
     2341 atggtctgta cgtcaaaccg ttgatgtgca ttaagccacg cattggcggg tgatgcaaca
     2401 attatttttc atatttatga ttaatcggtt ggaaaacggt cctgtcatca ggaccgtaaa
     2461 cagcaataaa gtggataaaa gtctattcca tcaactgctt acttaatgcc gggttggctt
     2521 gaatcaaacg catcaatttt agttcggcac ttgaaggctt cacgcgtctg gattcccatt
     2581 cctttaccat ggcgactgat acgcccaaaa cccgggcgaa atcatcgatt tttaatcctg
     2641 tcccttttcg taactgctca atttcggtac aggatgttgt tctgtgcgtc agggtaattt
     2701 tctgcgtttc atctttaaaa acaatctgtt ccaggctgct caacagctca tgcattggat
     2761 ctttatattc cattgaaaac tcctcaaatc acactgcggg atcgtgaatt gcatcgaagc
     2821 tcattaagaa tagtcgggaa aagcagacac agcggggtga agtcagggca tgctctggct
     2881 atgattcgat tcagccagtc atttagcgtg acttatagcc acctaacgat ctgataaatg
     2941 ccgatatatc tcttaccact cccggtttgt aaagatttcg aaaatctaac gttctgcgaa
     3001 ggcgtaaatc tcggtctaca aaaaaagcaa cggcactatc atttattaac aatgaaacaa
     3061 caaattaacc ttgtttcatt ttctccatga ttttaccagg aagattacgt agaaagggta
     3121 tcctgcaagc ctgtcctgga ctatccttgt cacgtcagac acgcgtgtgt cgttgtgcgc
     3181 tttttttggg tgaaaggagt aagaaaatgg cgacaggaaa gtcctgctct cgctggtttg
     3241 cgcctcttgc ggcgttatta atggtagtta gcctgagtgg gtgttttgat aaagaaggcg
     3301 atcagcgtaa agcgtttatc gacttcctgc agaatacagt gatgcgtagc ggtgaacgtc
     3361 taccaaccct gactgccgat cagaaaaaac agtttggtcc ttttgtctct gattacgcga
     3421 ttctgtatgg ttattctcag caggtgaatc aggcgatgga ttccggtctg cgcccggtcg
     3481 tagacagcgt taacgcaatt cgcgtaccgc aggattacgt tacgcaaagc ggcccactgc
     3541 gtgaaatgaa cggttctctg ggcgtactgg cgcaacagct gcaaaatgcg aagttgcagg
     3601 ccgatgctgc gcactctgcg ctaaaacaga gcgatgacct gaaaccggtc tttgatcagg
     3661 cattcaccaa agtggtaacg acgccagctg atgcattgca accgttgatt ccggcggcac
     3721 aaactttcac gcaacaactg gtgatggttg gagactacat tgcccaacaa ggtactcagg
     3781 tgagctttgt cgcaaatggc atccagttcc cgacgtcaca gcaggcaagt gaatataaca
     3841 aactgattgc gccattaccg gcacagcatc aggcgtttaa tcaggcctgg actacagcag
     3901 ttactgcaac ccaataaaga gtaaaagccc gagcgattat cgggctttac tccacgcagt
     3961 cgcggcttag tccgcgacgt gcggattcac acagttcttc tcaacctttc cttgtaacgc
     4021 atcaatcaaa ttatccacgg cacaggcggc catgccataa cgcgtctcat gggtggcaga
     4081 tccaatatgc ggtactgcga cgacatttgc cattgagagc aacggcgaat ctacggaaag
     4141 tggctcttgt tcgaagacat ccagcccggc ggcgtgaatt tcgcctttct gcaatgcagc
     4201 aatcagtgca ttttcatcaa ccaccgggcc acgtccggca ttaatgaaaa tggcggagga
     4261 tttcattttg ccgaattgtt ctgcgccaaa tagatgatac gtctcatcag ttaacggcag
     4321 gatcaggcaa acgaaatctg actcttgtaa cagtgtatcc aaatcgcagt agcgggcgtt
     4381 gaagcgttct tctgcttctt tatggtggcg gcgcgcgtta tagaggatgg gcatgttgaa
     4441 accaaagtgc gcacgttgtg ccagcgccat accgatccgt cccatcccga caatgcccag
     4501 tgttttatgg tgaacgtcag tgccgtacca gtccgggcct atgctcgcgg tccattcgcc
     4561 tgcttttacc cgttctgcta cctccacaac ccgacgagcg gtagacaaca ccagcgccat
     4621 cagcgtatcg gcgacggttt ctgttaatac ggttggcgtg tgcatcacca gaatttttcg
     4681 ggcggtaagc gcatcgacat caaaattgtc atagccgaca gagatcgttg atgtggcacg
     4741 cagtttcggc attttttcca gcaatgcggc atcaacattc tcgtttgaac ccagtaaacc
     4801 ttcagcttcg gcaaaaattg ctgcattttg ttcgacggtt tgtgggctga ggtttgccac
     4861 ctggtgaacg gtgaaatgct cttgcaggcg ttgcagtaaa tcatcaggta aggctttgta
     4921 gaggataacg gacggcttca tgcttctctc cattcactga taaggcgatc accttagcct
     4981 gaaatatgat gaaggaaaac tgaagaatgc caggtgattt ctgcatcacc tggcatgaaa
     5041 gggattacag cgggcttaag gtaatttcta cacggcggtt ttgcgcctta ccttctgcag
     5101 tgctgttgct ggcgattggg ttagccgggc caaggccctg agtacggatg cggctggcgt
     5161 ccacgccctg ggtgatcaac gcgctggcaa cggaatccgc acgctgctgg gagagacgca
     5221 tgttcaggtc gtgaccaccc gtgctgtcgg tataaccaat cacgttaacc gccgttttcg
     5281 gatactcttt cagcaccatt gccacgccgg tcagggtgtt agcgcctgcc ggtttcaggg
     5341 tcgcgctgct gctgtcgaag gtcacattgt tcggcatatt gaggataatg ttatccccgc
     5401 tgcgggttac gctaacacca gtgccacgca ttttgtcacg cagcttcgct tcctgcacat
     5461 ccatgtaata acccacgccg ccgcccagag ctgcacccgc tgctgcgcca atcagcgcgc
     5521 ctttaccgcg atctttcttc gaagaagaga gcgcaccaat acccgcgcca acgagagagc
     5581 ccagacctgc gccgatagca gatttacctg cttcgcgttc gccggtgtaa gggttagttg
     5641 tgcagccaga taccgccaga gcgccactca ctacggcggc aataagataa acacgtttct
     5701 tcattgttaa tccttaataa cctttttatt ctttgccacg ggttccgtgg cgggagatta
     5761 tgccgcgtga acatgaagat tattcctggg aatactcgga aatttgtaag taatatttaa
     5821 ctgctcaata catctaacct ttcaggagtc ttcggtttgg ccaactcatc ctcacgatat
     5881 tccgttctga ctgccgccca ctgggggccc atgctggttg aaactgacgg cgaaaccgtg
     5941 cttagctcgc gtggcgcgtt aaccacagga atggaaaact ccttgcagag cgcggttcgc
     6001 gaccaggttc acagcaatac gcgggtacga tttccaatgg tgcgcaaagg ctttctcgcg
     6061 tcaccggaaa aaccgcaagg cattcgcggg caggatgaat ttgttcgtgt gagttgggat
     6121 gaggcgctgg agcttattca ccaacaacat aaacgcattc gtgaggctta tggtccggca
     6181 tcgatttttg ctggttccta cggctggcgt tcaaacggcg tgctgcataa ggcctcgaca
     6241 ttattacaac gctatatggc gctggcaggc ggttataccg ggcatctggg ggattattcg
     6301 accggtgcag cacaggcgat catgccgtat gtcgtggggg gcagcgaagt ttatcaacag
     6361 caaaccagtt ggcccctggt gctggaacat agcgatgtcg tggtgctgtg gagtgctaat
     6421 ccactcaata cgctgaaaat tgcgtggaat gcatccgatg agcaggggct ttcttacttt
     6481 tccgcactgc gtgacagcgg gaaaaagctg atctgcattg atccaatgcg atcggaaacc
     6541 gtcgatttct ttggcgataa aatggagtgg gtggcaccgc acatgggcac cgatgttgcg
     6601 ctgatgctgg ggatcgccca tacgctggtg gaaaatggtt ggcacgacga agcgtttctg
     6661 gcgcgttgca ccaccggtta tgccgtcttc gcctcttatt tgctgggcga gagtgacgga
     6721 atagccaaaa ccgccgaatg ggcggcaggg atttgtggag ttaacgcaga gaaaatccgc
     6781 gagctggcgg ctcttttcca ccaaaacacg accatgctga tggctggctg gggaatgcag
     6841 cgtcaacagt ttggtgagca aaaacactgg atgatcgtca cgctggcggc aatgttgggg
     6901 caaatcggca cacccggcgg cggttttggt ctttcttatc attttgccaa tggtggtaac
     6961 cccacgcggc gttctgcggt gctctcttcc atgcagggca gcttgcctgg cggcaccgat
     7021 gcggtagata aaatccctgt tgcccgcatt gttgaagcac tggaaaaccc tggtggcgca
     7081 tatcaacaca acggtatgaa ccgacatttc ccggatattc gttttatctg gtgggcgggc
     7141 ggtgccaact ttactcatca tcaggatacc aatcgcctga tccgtgcctg gcaaaaaccg
     7201 gagctggtgg tgatctctga atgcttctgg acggctgcgg cgaaacacgc ggatatcgtt
     7261 ctgcctgcga ccacttcgtt tgagcgtaat gatctcacca tgaccggcga ttacagcaat
     7321 cagcatctgg tgccgatgaa acaagtggtg ccgccacgtt atgaagcgcg taatgatttt
     7381 gatgtctttg ccgagttgag tgaacgttgg gagaagggcg gctatgcgcg gtttacggaa
     7441 ggaaaaagtg agctgcaatg gctggaaacg ttttataacg ttgcccggca gcgcggggca
     7501 agccagcagg ttgaattgtc gccatttgct gagttctggc aagccaacca gttaattgag
     7561 atgccggaaa acccggacag tgagcggttt attcgcttcg ccgattttcg ccgcgatccg
     7621 caggcgcatc cattaaaaac cgccagcggt aagattgaaa tattctcgca gcgtattgcc
     7681 gattatggtt accaggattg ccctgggcat ccgatgtggc tggagccgga tgagtggcag
     7741 ggcaatgccg aaccagaaca gttgcaggta ctttctgctc atccggcaca tcgtctgcac
     7801 agccagctga attacagttc tctgcgcgaa ttgtacgcgg tggcaaatcg tgagcctgtc
     7861 accattcatc ctgacgatgc ccaggcgcgc ggcatacaag atggcgatat tgttcggttg
     7921 tggaacgcac gcgggcaaat tcttgccgga gcggtcatta gcgagggaat taaacctggc
     7981 gtgatttgta ttcatgaagg ggcatggccg gatctggatt taaccgctga cggtatttgt
     8041 aaaaacggcg cggtgaacgt tctgaccaaa gatctcccca gctcgcggct ggggaatggc
     8101 tgtgcgggta atacggcact ggcatggctg gaaaaataca acggtccgga actgacactt
     8161 acagcgtttg aaccaccggc cagctcataa tccaggtggg tagttgggtt tcatcctgcc
     8221 atgcgcagtc gacaatgtga aaaccctgcg catggtaaaa atctatcgcc ggttgatttt
     8281 tttgataaac ctccagcatc agatggggat ggcgctgctg cacatactgc attagcgcct
     8341 taccaatacc gcgtctgacg gccttcggtg cgacaaacat tgcagccaga aatcggcctt
     8401 ccataatgct gacaaaaccg agaagcttac cgtcttcttc ccagacccag ttttgcgcat
     8461 tggcaagata ggcatcccgc accagcggaa tgcaatcacg ccagtaattc gcttttataa
     8521 agggatgccc ccaggttgta ctttccagcc acagttcgag gatcgcgggg agttctgaac
     8581 gttgcgcttc ccgaatcatg gtttatttcc cggatagcaa cagcagccaa ccacatgatc
     8641 attcaccagc ccacatgcct gcataaagga gtaacagatt gtggtaccaa caaacttaaa
     8701 accacgcttt ttcagggctt tagatagagc gtcggaggcg ggcgtagatg tagggatttc
     8761 gctcaacgtt gtggcttgtg tcacctgtgg ttgatgattg acaaacgacc agacaaagtc
     8821 ggcaaacggt tcgccgttct gttccatttg caggtacgcc cgcgcattac caataattgc
     8881 ctgaattttc ccgcgatggc ggataatccc ggcgtcctgt accagtcttt cgacatcctc
     8941 ttcctgcatt gcagccacct tcaccggatc gaactgatga aagctggcgc gatagttttc
     9001 gcgttttttg aggacggtga tccacgataa tccagcctgc tgcccttcaa ggcagatcat
     9061 ttcgaacagt tttttactgt cagtttcagg cacgccccac tcattatcat ggtaggcaat
     9121 ataaagcggg tcctgactca cccagccgca acgttccata ctttccctcg ctatgtaccc
     9181 gatcgtaaaa atttcagccg cttttcttga cgcatcggtg aaaaagtcaa tatttaatac
     9241 agggctacta tgcccgatat ccttcataca atgacaataa tattgccgaa ttcggctctt
     9301 ccctggagtc gtgttaatga tcataaaaaa aagcggtggt cgctggcagc taagcctgct
     9361 ggcgagtgtg gtaatcagtg ccttttttct caacacagct tacgcctggc aacaagaata
     9421 tatcgttgat acgcaacccg gacattccac agagcgttac acctgggata gtgatcatca
     9481 acctgattac aacgatattt tgtcgcaacg tattcaaagt agccaaaggg cgctgggact
     9541 ggaagtcaat ctggcggaag aaactcctgt ggatgtgacc agcagtatga gtatgggctg
     9601 gaattttcct ttatatgagc aggttacaac cggcccggtc gcggcattac attacgatgg
     9661 cacaaccacc tcgatgtata acgagtttgg cgacagtact accacgctgg ccgatccgtt
     9721 atggcatgcc agcgtgagta ccttaggctg gcgtgttgac tcccggcttg gcgatctccg
     9781 accctgggcg caaatcagct ataaccagca atttggcgag aatatctgga aggcgcaatc
     9841 aggcctgagc cggatgacgg cgacaaacca gaacggcaac tggctggatg tcaccgtagg
     9901 cgctgatatg ttgctcaatc aaaatattgc tgcctatgct gcgctatctc aggcagaaaa
     9961 taccactaac aatagcgact atctgtatac gatgggggtt agtgccagat tttaacgtta
    10021 tagatacaat tgaaaccatt ggataacaac agttgtggcg atagtgggtg ctaacttact
    10081 aaataataaa tttggtgaat aattgtcgag tcattcattc ctgaactaat gcatttcatt
    10141 ccgttctgat ggcatttcat gccgtttttc cccaggcata aagtgcactt cgttatggtt
    10201 gtcagcagag atttttcctt tttattactg caggaatact gccatgacac cttcaaatta
    10261 tcagcgtacc cgctggttga cactcatcgg tactatcatt acccagtttg cgctggggtc
    10321 ggtttatacc tggagcctgt ttaacggcgc gctttctgcc aagctggatg cgccggtaag
    10381 ccaggtcgct ttctctttcg gcttgttaag tctggggctg gcaatttcat cttctgttgc
    10441 ggggaaatta caggaacgtt ttggcgttaa acgcgtcact atggcttccg gcattttgct
    10501 gggattaggc ttcttcctga cagcgcattc tgacaacctg atgatgctgt ggttaagcgc
    10561 cggtgtgctg gtcggtctgg cggatggcgc gggttacctg ctgacgctct ctaactgcgt
    10621 gaagtggttc ccggagcgta aaggtctgat ctccgcgttc gctatcggtt cttatggtct
    10681 gggtagcctg gggttcaaat ttatcgacac gcagctgctc gaaacggtgg gtctggaaaa
    10741 aacatttgtg atttggggag cgattgcgct ggtgatgatt gttttcggcg caacgttaat
    10801 gaaagacgca ccaaaacagg aagtgaaaac cagcaatggt gtcgtggaga aagattacac
    10861 gctggcagag tcgatgcgta aaccgcagta ctggatgtta gcggtaatgt tcctgactgc
    10921 ctgcatgagc ggcctgtacg tgattggggt agcgaaagat atcgcccaaa gtctggcaca
    10981 ccttgatgtg gtttccgcag ccaatgcagt cactgttatt tccatcgcca acctttcagg
    11041 tcgtctggtt ctggggatac tgtctgacaa aatcgcccga atccgtgtta tcaccattgg
    11101 tcaggtgatt tcgctggtgg gtatggcggc cctgctgttt gcaccattga atgcagtgac
    11161 gttctttgca gcgattgcct gcgtggcgtt taactttggc ggcactatca cggtgttccc
    11221 gtcactggtc agtgagttct tcggcctcaa taacctggcg aaaaactacg gtgtgattta
    11281 tctcggtttc ggtatcggca gcatttgtgg gtcgattatc gcctcactgt ttggcggctt
    11341 ctatgtgacc ttctacgtga ttttcgccct gctgattctg tcactggcgc tctctacgac
    11401 gattcgtcag ccagagcaga aaatgttgcg tgaggcgcat ggctcccttt aatcatctgc
    11461 cgcgagctac tgggctttag ccttagtgtg aatcaaacgc ttcaacgcaa tgtataaaca
    11521 atgacgcgta aaatttgggc cagttaactg gcccttttta ctgctttttg taacgctaaa
    11581 gtctcttttc aaacttgcat ttttgtaaat ttgtgcttcg tgcacactct ttccccacac
    11641 tttttccctt tgctgtggtc tacttattcg cgcgtgtaga ttttacttat ctgactacct
    11701 ccgcactttt cccctgccgg gcctgaaaag ccgttaagca gggtgttatc acctgtttgt
    11761 ccagggtttg tttgcatgag atacatcaaa tcgattacac agcagaagct gagctttttg
    11821 cttgcaatct atattggcct ttttatgaat ggcgcggttt tttaccgccg cttcggcagc
    11881 tatgcgcacg attttaccgt ctggaaaggc atttctgctg ttgttgaact ggccgccacc
    11941 gtactggtga ccttcttttt actgcgtctt ctttcgctgt ttggccgccg cagctggcgt
    12001 attctggcat cgctggtggt gctcttttcc gcaggtgcca gctattacat gaccttcctt
    12061 aatgtagtca ttggttatgg catcatcgca tccgtcatga ccaccgatat cgacctgtca
    12121 aaagaagttg ttggtctgaa ctttattctc tggttaatcg ccgttagtgc attgcctctt
    12181 atccttatct ggaataaccg ctgtcgctac accttgctcc gacaactgcg aaccccaggg
    12241 cagcgtattc gcagcctggc ggtcgtcgta ctggcgggta ttatggtttg ggcaccgatt
    12301 cgtttgctgg atatccagca gaagaaagtg gagagggcga ccggcgttga tttgccgagt
    12361 tatggcggtg tcgtagcgaa ctcttatctg ccatcaaact ggctttctgc gttggggctg
    12421 tatgcctggg cgcgggtcga tgaatcttcc gataacaatt cattgcttaa tccggcgaag
    12481 aaattcactt atcaggcacc gcaaaacgtt gatgacactt atgtcgtgtt tatcatcggt
    12541 gaaaccacgc gttgggacca tatgggcatt ttcggctatg agcgtaatac cacgccgaag
    12601 ctggcccagg agaaaaatct ggcggcgttc cgtggctatt cttgtgacac ggcgaccaaa
    12661 ctttctctgc gttgcatgtt tgtacgagag ggtggagcgg aagataatcc gcagcgcaca
    12721 ttaaaagaac agaacatttt cgcggtgctg aagcagttag gattcagttc tgacctctac
    12781 gctatgcaga gcgaaatgtg gttctacagc aacacgatgg cagacaacat tgcttatcgt
    12841 gagcagattg gtgcggagcc acgtaaccgt ggcaagccgg tagatgatat gttgctggta
    12901 gacgaaatgc agcaatcgct ggggcgcaac ccggatggca agcatctgat cattttgcat
    12961 accaaaggtt cgcactttaa ctacacccag cgttacccgc gcagctttgc gcagtggaag
    13021 ccggaatgta ttggtgttga tagcggctgt accaaagcgc agatgatcaa ctcctatgac
    13081 aactcggtga cctatgtgga tcactttatc tccagcgtga ttgatcaggt tcgcgataag
    13141 aaagcgattg tgttctacgc agctgaccac ggtgagtcaa ttaatgaacg cgagcacctg
    13201 cacggcacac cgcgtgaact ggcaccgccg gagcagttcc gtgtgccgat gatggtctgg
    13261 atgtcagata aatatctgga aaatccggtc aatgcacagg catttgcgca gctgaaaaaa
    13321 gaagccgaca tgaaagtgcc acgccgtcat gtagaactgt acgacaccat catgggttgt
    13381 cttggctata cttcaccgga tggtggaatt aacgaaaaca acaactggtg ccacatcccg
    13441 caggcaagag aggcggcggc taactaagag gcctgctgac tttctcgccg atcaaaaggc
    13501 attttgctat taagggattg acgagggcgt atctgcgcag taagatgcgc cccgcattcg
    13561 gtgattggcg cagcctggta gcgcacttcg ttcgggacga aggggtcgga ggttcgaatc
    13621 ctctatcacc gaccaaattc gaaaagcctg ctcaacgagc aggctttttt gcatctgcag
    13681 cccatgagga tgagaacctc cgggggcagg gaggttcgac tcgagcgaag cgagagaacg
    13741 ttgcgccagc aacggcccgc agggcgagcc acgaagtggc gagtaatcct ctatcaccga
    13801 ccaaattcga aaagcctgct caacgagcag gcttttttgc atttgtagcc tatgaggatg
    13861 agaacctccg ggggcaggga ggttcgactc gagcgaagcg agagaacgtt gcgccagcaa
    13921 cggcccgcag ggcgagccac gaagtggcga gtaatcctct ggcaccgacc aaattcggca
    13981 ccgcactcat tgtgggatac agcgtatccg tcttatccga tctaaacggc aacactccac
    14041 acatttccct gccaaatttg cgccccatat ctcactatcc attaacggat ttgtgacaga
    14101 aaccattgtc tttttttata tttgcttaaa tctttttttg cgttacttat ggctggcgct
    14161 ggcattttcc gctgtgcggt ttagcgttca tggtattaat cgctcagata tccacctttt
    14221 catcagattt ttttcacaaa atgcataaaa cttaatcacc aattgttgct aattattaag
    14281 ctgattgttc tggttatgtt ggagtagcac aaatttctct gctgcaaata gctgtttgaa
    14341 gtttttttaa tctttgtttg tggattctca ccgttggtgt aaatcccggt gggctgtatt
    14401 gacgttttca cattctgttg acagattgta ggtcacgagg ggcattttat ggaggatccg
    14461 cactgtaaca ctgatgttaa ttagtacggc atccccacct cataacgttg acccgaccgg
    14521 gcaaaaaaca aaaaaggtca ggcagcgaca acccactgca aagggttaaa acaacaaaca
    14581 tcacaattgg agcagaataa tgcgtatttc cttgaaaaag tcagggatgc tgaagcttgg
    14641 tctcagcctg gtggctatga ccgtcgcagc aagtgttcag gctaaaactc tggtttattg
    14701 ctcagaagga tctccggaag ggtttaaccc gcagttgttt acctccggta ctacctatga
    14761 cgcctcttcc gtgccgcttt ataaccgcct ggttgaattt aaaatcggca ccaccgaagt
    14821 gattccgggc ctcgctgaaa agtgggaagt cagcgaagac ggaaaaacct ataccttcca
    14881 tctgcgtaaa ggtgtgaagt ggcacgacaa caaagaattc aaaccgacgc gcgaactgaa
    14941 cgccgatgac gtggtgttct cgttcgatcg tcagaaaaac gcgcaaaacc cgtaccataa
    15001 agtctctggc ggcagctacg aatacttcga aggcatgggc ttgccggagc tgatcagcga
    15061 agtgaaaaag gtggacgaca acaccgttca gtttgtgctg actcgaccgg aagcgccgtt
    15121 cctcgctgac ctggcaatgg acttcgcctc tattctgtca aaagaatatg ctgacgcgat
    15181 gatgaaagcc ggtacaccgg aaaaactgga cctcaaccca atcggtaccg gtccgttcca
    15241 gttacagcag tatcaaaaag attcccgtat ccgctacaaa gcgtttgatg gctactgggg
    15301 caccaaaccg cagatcgata cgctggtttt ctctattacc cctgatgctt ccgtgcgtta
    15361 cgcgaaattg cagaagaatg aatgccaggt gatgccgtac ccgaacccgg cagatatcgc
    15421 tcgcatgaag caggataaat ccatcaacct gatggaaatg ccggggctga acgtcggcta
    15481 tctttcatat aacgtgcaga aaaaaccact cgatgatgtg aaagttcgcc aggctctgac
    15541 ctacgcggtg aacaaagacg cgatcatcaa agcggtttat cagggcgcgg gcgtggcagc
    15601 gaaaaacctg atcccgccaa ccatgtgggg ctataacgac gacgttcagg actacaccta
    15661 cgatcccgaa aaagcgaaag ccttgctgaa agaagcgggc ctggaaaaag gcttctccat
    15721 cgacctgtgg gcaatgccgg tacaacgtcc gtataacccg aacgctcgcc gcatggcgga
    15781 gatgattcag gcagactggg cgaaagtcgg cgtgcaggcc aaaattgtca cctacgaatg
    15841 gggtgagtac ctcaagcgtg cgaaagatgg cgagcaccag acggtaatga tgggctggac
    15901 tggcgataat ggggatccgg ataacttctt cgccaccctg ttcagctgcg ccgcctctga
    15961 acaaggctcc aactactcaa aatggtgcta caaaccgttt gaagatctga ttcaaccggc
    16021 gcgtgctacc gacgaccata acaaacgcgt tgaactgtac aagcaagcgc aggtggtgat
    16081 gcacgatcag gctccggcac tgatcatcgc tcactccacc gtgtttgaac cggtacgcaa
    16141 agaagttaaa ggctatgtgg ttgatccatt aggcaaacat cacttcgaaa acgtctctat
    16201 cgaataatta aaagccatac ccggtggcgc tgtgcctctg cgtgcagcgc catccggcag
    16261 caatacttca ttccctgccc gacctgggcg ggaattgatt tgtgagcaat acagacacgc
    16321 agttccaggc tgcgggtcac tacagagaat ccgggttatg ttgcagttta ttctccgacg
    16381 tttgggactc gtcatcccca cgtttatcgg tattaccctt ctcacatttg cctttgtcca
    16441 catgatcccg ggcgatccgg tgatgatcat ggcgggcgaa cgtgggatct ccccagagcg
    16501 tcacgcgcag ctgctggctg aactcggctt agataaaccg atgtggcagc agtatctcca
    16561 ttacatttgg ggcgtaatgc acggcgattt aggcatttcg atgaaaagcc gcatcccggt
    16621 atgggaagag ttcgtgccgc gctttcaggc cacgctggaa cttggcgtct gcgcgatgat
    16681 ttttgctacg gcagtcggta ttccggttgg tgtgctggct gcggttaaac gcggttccat
    16741 tttcgatcac acggcggttg gcctggcgct gacaggttat tcaatgccta tcttctggtg
    16801 gggcatgatg ctgatcatgc tggtttcggt gcactggaac ctgacgcccg tctccggtcg
    16861 cgtgagcgac atggtgttcc tcgatgattc caatccgtta accggtttta tgctaatcga
    16921 caccgccatc tggggtgaag acggtaactt tatcgatgcc gtcgcccata tgatcctgcc
    16981 cgccattgtg ctgggtacta tcccgctggc ggtcattgta cgtatgacac gctcctcgat
    17041 gctggaagtg ctgggtgaag attatatccg caccgcgcgc gccaaagggt tgacccgcat
    17101 gcgggtgatt atcgtccatg cgctgcgtaa cgcgatgctg ccggtagtga ccgttatcgg
    17161 cctccaggtg ggaacattgc tggcgggggc gattctgacc gaaaccatct tctcgtggcc
    17221 cggtctgggg cgctggttga ttgacgcact gcaacgccgc gactatccgg tagtgcaggg
    17281 cggcgtattg ctggtggcga cgatgattat cctcgtcaac ttgctggtcg atctgctgta
    17341 cggcgtggtg aacccgcgta ttcgtcataa gaagtaaggg gacatcatgt cacaggttac
    17401 tgaaaataaa gtgattagcg caccggtgcc gatgaccccg ttacaggagt tctggcacta
    17461 ttttaaacgc aacaaaggcg cggtcgtcgg gctggtttac gtcgtcatcg tgctgtttat
    17521 cgcgatcttt gccaactgga ttgcacccta taacccggcg gaacagttcc gcgatgcact
    17581 gctcgccccg ccagcctggc aggaaggcgg cagcatggcg cacttgctgg gcaccgatga
    17641 cgtaggccgt gatgtgctgt cgcgtctgat gtacggtgcg cgattgtcac tgctggttgg
    17701 ctgtctggta gttgtgttat cgctgattat gggcgttatt ctcggcctga tcgccggtta
    17761 cttcggaggc ctggtcgata acatcatcat gcgcgtggtc gatatcatgc tggcgctgcc
    17821 aagtttgctg ttggcactgg tgctggtggc aattttcggc ccgtcgattg gtaacgccgc
    17881 gctggcattg accttcgttg ccttgccgca ctatgtgcgc ttaacccgcg ccgccgtgct
    17941 ggtggaggtg aatcgcgatt acgttaccgc gtctcgcgtg gcaggtgccg gggcgatgcg
    18001 tcagatgttt attaacatct tcccgaactg ccttgcgcct ctgattgttc aggcgtcgct
    18061 cggtttctct aacgccattc tcgatatggc tgctcttggt ttcctcggca tgggggcgca
    18121 gccgccaacg ccggagtggg gcaccatgct ctccgacgtg ttgcagttcg cgcaaagcgc
    18181 ctggtgggtc gtgaccttcc cgggtctggc gatcctgctg acggtgctgg catttaacct
    18241 gatgggtgac ggtctgcgtg acgcgctcga tcccaaactg aagcagtaag aggttcgaga
    18301 tggcgttatt aaatgtagat aaattatcgg tgcatttcgg cgacgaaagc gcaccgttcc
    18361 gcgccgtaga ccgcatcagc tacagcgtaa aacagggcga agtggtcggg attgtgggtg
    18421 agtccggctc tggtaaatcg gtcagttcgc tggcgattat ggggctgatt gattatccgg
    18481 gccgcgtaat ggcggaaaag ctggagttta acggtcagga tttacagcgt atctcagaaa
    18541 aagagcgccg caacctggtg ggtgccgaag tggcgatgat cttccaggac ccgatgacca
    18601 gccttaaccc gtgctacacc gtgggtttcc agattatgga agcgattaag gtgcatcagg
    18661 gcggcaacaa aagtacccgc cgtcagcggg cgattgacct gctgaatcag gtcggtattc
    18721 ccgatccggc atcgcgtctg gatgtttacc cgcatcagct ttccggcggc atgagccagc
    18781 gcgtgatgat cgccatggcg attgcctgtc gaccaaaact gctgattgcc gatgagccga
    18841 ccactgcgct ggacgtgacc attcaggcgc aaatcatcga actgttgctg gagctacagc
    18901 agaaagagaa catggcgctg gtgttgatta cccatgacct ggcgctggtg gcggaagcgg
    18961 cacataaaat catcgtgatg tatgcaggcc aggtggtgga aacgggcgat gcccacgcca
    19021 tcttccatgc gccgcgtcat ccatatactc aggcattgct gcgtgcgctg ccggaatttg
    19081 ctcaggacaa agaacgtctg gcgtcgttgc caggtgttgt tcccggcaag tatgaccgcc
    19141 cgaacggctg cctgcttaac ccgcgctgcc cctatgccac tgacagatgt cgcgctgaag
    19201 aaccggcgct gaatatgctc tctgacgggc gtcagtccaa atgccattac ccacttgatg
    19261 atgccgggag gccgacacta tgagtacgca tgaggccacc tcgcaacaac cgctgttgca
    19321 ggctatcgac ctgaaaaaac attatccggt gaagaaaggt atgttcgcgc cggaacgtct
    19381 ggtgaaggcg ctggacggcg tttcgtttaa ccttgaacgt ggcaaaacgc tggcagtagt
    19441 aggtgaatct ggctgcggta aatcgaccct cggtcggttg ctgacgatga tcgaaatgcc
    19501 caccggtggt gagctgtatt accaggggca ggatctgctc aagcacgatc cgcaggcgca
    19561 gaagctgcgt cggcagaaaa tccagattgt cttccagaac ccttacggtt cgctgaatcc
    19621 acgtaaaaaa gtcgggcaaa ttcttgaaga gccgctgctt attaatacca gcttaagcaa
    19681 agaacagcgt cgggaaaaag ccctgtcgat gatggcgaaa gtcggcctga aaaccgaaca
    19741 ttatgaccgc tatccgcata tgttctccgg cggtcagcgt cagcgtatcg ccattgcccg
    19801 tggtctgatg ctcgacccgg atgtggtgat tgccgatgaa ccggtttccg cgctggacgt
    19861 ttcagtgcgt gcgcaggtgc tgaatctgat gatggatttg cagcaggagt tggggctgtc
    19921 ttatgtcttt atctcgcacg acctgtcagt ggttgagcac attgccgatg aagtgatggt
    19981 gatgtacctg ggccgctgcg tggagaaggg aacgaaggac caaatcttca ataatccgcg
    20041 tcatccgtac actcaggcgc tactctccgc gacgccgcgc ctgaacccgg acgatcgccg
    20101 cgagcgcatc aagctcaccg gtgaactgcc aagcccgctc aatccaccgc cgggctgcgc
    20161 gtttaacgcc cgctgtcgtc ggcgcttcgg cccctgcact cagttgcagc cgcagctaaa
    20221 agactacggc ggtcaactgg tagcttgttt tgctgttgat caggatgaaa atccgcagcg
    20281 ttaactcaca aaaccggggc catgcgctcc ggtttactca ataagcttca acagtggcga
    20341 caagcataac aaaatgccgt acagcagaat cagccaggct ttaaacccgc gaagtttttc
    20401 cagttgtgcg actttataga tcaggaaaaa tggaatgagg cacgacacaa tgccatataa
    20461 cgggcttccc aactgaaaga acaccagcac cgatacacga aacgaaaccc aaatcgtcaa
    20521 cgtaatgacg ataaaagcgc agatcgccag agtcagcatg cgtgagttaa ttttcttggt
    20581 atcaataatt cggcttaaca gattgagaat aatgccttta atagcctcgt ggaaaccgag
    20641 ataaatgcca aagaatgcgg tcagtacggc aaagatattc agcaccgtag aggtgatatg
    20701 aatgatatgc ccagggatca cctgcgcggc cagtgccagt gccgagatat tttgttcaaa
    20761 ggcagaaacg gcttcttcgt ggctaattga gaaagtaaac gaaaaggcaa aaaacaggat
    20821 caccgcgatg agcgtgatat aactaattcg gtgggtacgc agcgcgagcc gggttgccag
    20881 tactttatct gcttcccgtt tacgataggc aatattcatt gggttaagta cctgaataaa
    20941 tactgcagaa aagaagcaaa atggaatggt aagcaggaca tcgcggaaaa agacggaggc
    21001 ttgcgggaag gcggttatat tggtgaaatt ccagtgcggg atcatcgcaa aaccgaacac
    21061 gacgataatc cctactttga ccaccaccat tggcccggaa atcttaaaca gtaatcgttc
    21121 accaccagac gcaattgcca ccagtacggc gaaaatggcg actttataaa gtagagattg
    21181 tgaaagatcg gcatcggtta aaccgaaggt tttcaggtac gaggcgctgt cgaaaaccac
    21241 ggagagagag tagataaaaa tcccgtggat aatcatcaaa aagtagataa ccccgaggaa
    21301 aattccccag ttcttcccca ggtaatgact gataatatcg gtgtagtcat tacaggaatc
    21361 gctttcagaa agggttttta aataaatgtc ctgcactacc caggtggcag gataagcaat
    21421 gatcgctgcg gtaataaata cccaaattcc cttcaagcca atttgtactg gcatcagcac
    21481 ggttccggca ccaatcgcca tgcctatgca taataaaacc cagccgaagt cgtagcgtgt
    21541 aaacggcaat ttctgatcgt gtttcggtag tgtgttgtgc tgcataaagt cctgtccaga
    21601 aagaagtcgg cctcgcaaaa atgaataaac ccagtttctc ttgcgcaaca ggtggagaag
    21661 tcgaatacgc agtatgtaaa aaatgaggca acagattcac tggggaggtc gcgaggaggc
    21721 gcaattctac aaaattgcgc atttcactac cagtctggag tcagctcgga tatgaactgc
    21781 aactattgca aataaagaaa ttaagctgga aggcatcgcg caatgaggaa gattgccggg
    21841 tgctgacggt aaaaagcaat cgtaaaatca gcggcaagcc ggaaaggttc cggtgaggcg
    21901 caatgttgcg ggggcttaat ccctggtggc attggttgct ggagagagaa aacccccgca
    21961 cgttgcaggt atgcacctga caacaccacg ggggccattc ttgcatccaa acaatacaag
    22021 aatagccgcg aaacgttgtc attacaactc aggcggctat atgacgctcg cagagctggg
    22081 catggccttc tggcatgatt tagcggctcc ggtcattgct ggcattcttg ccagcatgat
    22141 cgtgaactgg ctgaa
//
LOCUS       DACBVI010000066        22002 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.066, whole genome shotgun sequence.
ACCESSION   DACBVI010000066 DACBVI010000000
VERSION     DACBVI010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.066"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(1..510)
                     /gene="sulA"
                     /locus_tag="IU325_12370"
     CDS             complement(1..510)
                     /gene="sulA"
                     /locus_tag="IU325_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HAP5809815.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVLASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            729..1358
                     /gene="sxy"
                     /locus_tag="IU325_12375"
     CDS             729..1358
                     /gene="sxy"
                     /locus_tag="IU325_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HAP5809816.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(1321..3483)
                     /gene="yccS"
                     /locus_tag="IU325_12380"
     CDS             complement(1321..3483)
                     /gene="yccS"
                     /locus_tag="IU325_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HAP5809817.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILTFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(3493..3939)
                     /locus_tag="IU325_12385"
     CDS             complement(3493..3939)
                     /locus_tag="IU325_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP5809818.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            4062..6116
                     /gene="helD"
                     /locus_tag="IU325_12390"
     CDS             4062..6116
                     /gene="helD"
                     /locus_tag="IU325_12390"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HAP5809819.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPGEKVVRLHGTEWGETQRFYHHLDAHWRRWSSEMSEIASGV
                     LQQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWCKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLETTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESHLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(6148..6606)
                     /gene="mgsA"
                     /locus_tag="IU325_12395"
     CDS             complement(6148..6606)
                     /gene="mgsA"
                     /locus_tag="IU325_12395"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP5809820.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(6702..7364)
                     /locus_tag="IU325_12400"
     CDS             complement(6702..7364)
                     /locus_tag="IU325_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HAP5809821.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            7537..7950
                     /locus_tag="IU325_12405"
     CDS             7537..7950
                     /locus_tag="IU325_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP5809822.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLAEVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(7995..8312)
                     /gene="hspQ"
                     /locus_tag="IU325_12410"
     CDS             complement(7995..8312)
                     /gene="hspQ"
                     /locus_tag="IU325_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HAP5809823.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(8370..9560)
                     /gene="rlmI"
                     /locus_tag="IU325_12415"
     CDS             complement(8370..9560)
                     /gene="rlmI"
                     /locus_tag="IU325_12415"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HAP5809824.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWIFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPNESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGV
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            9655..9933
                     /locus_tag="IU325_12420"
     CDS             9655..9933
                     /locus_tag="IU325_12420"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP5809825.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKKLGLAGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSEELTDFRIR"
     gene            complement(9930..10259)
                     /gene="tusE"
                     /locus_tag="IU325_12425"
     CDS             complement(9930..10259)
                     /gene="tusE"
                     /locus_tag="IU325_12425"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HAP5809826.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(10350..11009)
                     /gene="yccA"
                     /locus_tag="IU325_12430"
     CDS             complement(10350..11009)
                     /gene="yccA"
                     /locus_tag="IU325_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HAP5809827.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(11216..11303)
                     /locus_tag="IU325_12435"
     tRNA            complement(11216..11303)
                     /locus_tag="IU325_12435"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11267..11269),aa:Ser,seq:tga)
     gene            11730..12848
                     /gene="hyaA"
                     /locus_tag="IU325_12440"
     CDS             11730..12848
                     /gene="hyaA"
                     /locus_tag="IU325_12440"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HAP5809828.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            12845..14638
                     /gene="hyaB"
                     /locus_tag="IU325_12445"
     CDS             12845..14638
                     /gene="hyaB"
                     /locus_tag="IU325_12445"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HAP5809829.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALKLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPTTWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELVSVQVR"
     gene            14657..15364
                     /gene="hyaC"
                     /locus_tag="IU325_12450"
     CDS             14657..15364
                     /gene="hyaC"
                     /locus_tag="IU325_12450"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HAP5809830.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDVHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            15361..15948
                     /locus_tag="IU325_12455"
     CDS             15361..15948
                     /locus_tag="IU325_12455"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HAP5809831.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKISLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMRLEEQG"
     gene            15945..16343
                     /gene="hyaE"
                     /locus_tag="IU325_12460"
     CDS             15945..16343
                     /gene="hyaE"
                     /locus_tag="IU325_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HAP5809832.1"
                     /translation="MSNDTPFNALWQRMLARGWTPISECRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            16340..17197
                     /locus_tag="IU325_12465"
     CDS             16340..17197
                     /locus_tag="IU325_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HAP5809833.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGDKLLEDKLEAGCAPLVLWQAATQNVLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPS"
     gene            17331..18875
                     /gene="appC"
                     /locus_tag="IU325_12470"
     CDS             17331..18875
                     /gene="appC"
                     /locus_tag="IU325_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HAP5809834.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQVAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            18887..20023
                     /locus_tag="IU325_12475"
     CDS             18887..20023
                     /locus_tag="IU325_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAP5809835.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGAIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            20036..20128
                     /gene="cbdX"
                     /locus_tag="IU325_12480"
     CDS             20036..20128
                     /gene="cbdX"
                     /locus_tag="IU325_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HAP5809836.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            20208..21518
                     /locus_tag="IU325_12485"
     CDS             20208..21518
                     /locus_tag="IU325_12485"
                     /EC_number="3.1.3.2"
                     /EC_number="3.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AppA family phytase/histidine-type acid
                     phosphatase"
                     /protein_id="HAP5809837.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAHSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLTKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPSQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACALHQDK"
     gene            complement(21506..21718)
                     /gene="cspH"
                     /locus_tag="IU325_12490"
     CDS             complement(21506..21718)
                     /gene="cspH"
                     /locus_tag="IU325_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HAP5809838.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         5359 a         5521 c         5920 g         5202 t
ORIGIN      
        1 ttaatgatac aaattagagt gaatttttag cccggaaagt tgtctcgttg cgtgagagga
       61 tgcgcttacc ggacgcataa taaaccccat agcgttacct tcatttgccg catcaacaag
      121 ttcagcatgc tcttcttcag tcaaatcatc tgccaaccaa ccgatcacca cactgtaatt
      181 gcccgtgcgt aaagcgcgaa ccattgactc cacggtgtgg caaggggaga gctggctaat
      241 ctgcatcact ttcgttaagg gtagcccaga tgccagaacc cattcccgac tcagtttttg
      301 ttgcggtgtt aaccagagtt gccagcgcga ttgctgaccg agttgctgta acaatggcaa
      361 cagtagaagt tgcgtcatca tgggctgatc ttcgcgatag acaacttcac tgataagccc
      421 ggctgtagtg ttttccgtag agacacgcgc aattttactt gctgcggatg agaacgacga
      481 agaacgatgt gcatagcctg aagtgtacat aatcaatcca gcccctgtga gttactgtat
      541 ggatgtacag tacatccagt gacaacaaag atcaacccta ttttcggaaa gagcctcgca
      601 aattttgtcg ttggtgacgg gaaaacataa attaatcttg ccccttaaga ataagttgcc
      661 tattttcgta gttaacggat ccgttaatgt gaatcattct tttatgttat gattttaaaa
      721 ggaattttat gaaaagcctc tcctataagc ggatctataa atcacaagaa tacctggcaa
      781 cgttgggcac aattgaatac cgatcattgt ttggtagtta cagcctgacc gttgacgaca
      841 cggtgtttgc gatggtttct gatggtgagt tgtatcttcg ggcttgtgag caaagtgcac
      901 agtactgtgt aaaacatccg cctgtttggc tgacatataa aaagtgtggc cgatccgtta
      961 ccctcaatta ctatcgggtt gatgaaagtc tatggcgaaa tcaactgaag ctggtgcgtc
     1021 tgtcgaaata ttctctcgat gcagcgctga aagagaaaag cacgcgcaat acccgggaaa
     1081 gactgaaaga tttgcccaat atgtcttttc atctggaagc gattctcggg gaggtgggga
     1141 ttaaggatgt acgggcgtta cgtatacttg gggcaaaaat gtgttggttg cgactgcggc
     1201 agcaaaacag tctggtgaca gaaaagattc tgtttatgct tgaaggggcc attatcggca
     1261 ttcatgaagc tgcgctcccg gtggcacgcc gccaggagct tgcagaatgg gctgactctc
     1321 ttacgccgaa acaggagttt cctgcggaac ttgagtaatc tggcgttgca gacgaccaat
     1381 ctctggcagt aatgcaatca ataacccaac ttgttgtacg accagaggtt ctttgctgtc
     1441 tgcccgcggt tcaagttgct gcatccgctg tttcaggcca gctaatgcct gattgacgcg
     1501 ttcttcatca gcaggttgat gatgtagcgc gtcatcaaca tagcaaactg cgtcatcaag
     1561 aaacgtcaga atttcaggat tagttaactg ctcccggtga gcaccgaggg ctgagatata
     1621 gctggtaaac gtatggttaa ggcacagcaa ccgaaacgcg gcctcgcgaa tttgcggggt
     1681 aacgttcggc tcgctggaca tatttgatac caccgacgcc agttcagcat cacggttgtg
     1741 tgcatcgcgg cgggcaatac gatacgccag acggttatca cgcccctgat ggtattgctc
     1801 cagtatggca tcgagatagc gacagttggc ctcggtggca cgttcgagca tgcgcggcag
     1861 attgcgaaac ttccagtcag gccagatgta gctcaccgcc gcccacgcaa tggcacaacc
     1921 aatcagcgta tcgattacgc gaggtaacgc cacttcaaaa ccttcaccca gcaagttaaa
     1981 acaaagtagc accaaaagtg tgatgaacat cgttgcatgg gcgtattgca cgttacggaa
     2041 ggcaaaaaag agcacgccgg tgataaccag tagcaccagt tgcccttcca gtgacggtac
     2101 aaaccacagc acaggaatgc caatggcgat acctaccagc gtaccaataa tccttaactt
     2161 caggcggtgg cgcgtggcgt tatagtttgg ctggcagaca aacaaacttg tcagcaagat
     2221 ccaatacccg tgatgcattc cggttatctg aatgatggcg tagccgaagc acaacaccag
     2281 cgacattctt accgcatgac ggaagagggc ggattccggc gtgaagtgac ggctaagacg
     2341 cagccagata tcactcaatc cgtgcgggct gtcatcagcg agctcatttt cgtcattatt
     2401 atggggcagt gcctgggcct gttctgattc aattgttgcc agttgggcat caatggcgcg
     2461 taaattgttc agcaaaaatc ccagtgtttt gagtaaatcg gcaggtgcgc cgttatcgcg
     2521 catccgctcc agcgcagcat caatatgcgt aaaagcgcgc tcgaaatgcg gatcatgttg
     2581 ataaggctga cgcaacaaaa tacagcgtga cagttgctgg cacgcctggc cctgcatcga
     2641 catcagccgc tgaaaacgga acagcacgtc gctgtggcga aaatgttcac gcaatgtttg
     2701 atactgaata tgagaagagc tggcacgctc gtgaatatcc tgcgcgacga agtaataatg
     2761 cagcgtgcga cgcgttcccc gttgaccacg atcgccacgt aagcgggtca gcagcgagag
     2821 tttcgtctga ttcaatgtcg ccatcagctg accgttggcg agagccaaat cgtacagcgg
     2881 tgcctggctt tcatcttcaa tatcaggatc aaacatgcgc gacttgagct caaggtaacg
     2941 cgccagttgt tcatagcaac gcgccaggtt gtcctgcagc gggcggaccg ggaacagtag
     3001 atgaccaata agtgtcagga cgttgtacca gacggcaccg gccagcagat acatcggctg
     3061 ctgataccag tgctcataca gtgatgttcc caacatagtg taaatggcga tcagcaatgc
     3121 accgaaggca attgttgcat agcgttgacc cagaccgccg agcaaaatga agccgctggt
     3181 agagagcgtt aagccaatcg caaatagcca gggccaggga aacagcaatt ctactgaggc
     3241 cgaggcgata aaaaagcaga acagcgtaat gatgaggtta cgcaaacgtc ccgccagtcg
     3301 gtcatcgaga tcggtcagcg ccgctgccac catccccagc gttagcggaa tcgtcagttt
     3361 tacatcaccc agccaccacg gaaacgctgt ggttccacaa agcgcaataa aaatacgcgc
     3421 gtaatacagc caggcgctgt tccaggtata gcgtttgagc aaaggactta gcataaaggc
     3481 catattagtg gattattcaa aacgacgacg tgcattggct tcacgcgcag cttgcgctgt
     3541 ttctaccgat accacgcgac gaccgaccgg ccatagtgca atagcggcaa ttttaaagtt
     3601 cgcaatgccg acaggaatgc caatgatgga aatacattgt gcgatgcccg ttgcaatgtg
     3661 catcaggcat aaccaccagc caaagaaaat cagccagaaa atattcaata ccgtaccgcc
     3721 agtatttagc agcacatttt tgccagccgg gttcagttca tcgacatgaa tagcttcatt
     3781 gccataaggc accagagaca gtttagtgat ctcccagcag gatcgtgtca gcggtaaggt
     3841 aaaaatcagc acaatactga ccagagtcgc caacagccag cccagagtgg tggcaaatcc
     3901 gccaagcaca aagttcagaa tgttcaaaac ggtacgcata aaacctcgct ttactgtggt
     3961 tttcagtaat gggcggcaat tgtaacgttt ttttgggctg gagcacgttt tctctgacgg
     4021 ttacactgat aagaaatcat ttcgtgtgga tctacagagt catggaactg aaagcgacaa
     4081 cgcttggaaa acgtctggca cagcaccctt acgatcgggc ggtgatcctc aatgccggga
     4141 ttaaagtctc cggcgatcgc cacgaatacc tcattccttt caatcaatta ctggcgattc
     4201 actgtaagcg cggtctggtg tggggcgagc tggaatttgt actgccgggc gaaaaagtgg
     4261 tgcgtctgca cggtactgaa tggggcgaga cgcagcgttt ttaccatcat cttgatgctc
     4321 actggcggcg gtggagtagc gagatgagcg aaattgcatc tggtgtttta cagcagcaac
     4381 tggatttaat tgccacgcgc accggagaaa ataaatggct gacgcgtgag caaacctctg
     4441 gcgtgcagca acaaatccgc caggctttgt cggcgttgcc gttgccggtt aaccgactgg
     4501 aagaattcga taactgccgt gaggcgtggc gtaaatgtca ggcctggttg aaagatattg
     4561 aaagcgctcg gttgcagcat aatcaggcat ataccgaagc catgcttacc gagtatgcgg
     4621 attttttccg ccaggtcgag tcttcaccgc tgaatccggc gcaggcccgg gcagtcgtta
     4681 atggcgagca ttctctgtta gtgctggcag gtgcaggaag cggaaaaacg tcggtgctgg
     4741 tagcccgtgc aggctggttg ctggcgcgtg gtgaagcgtc ccctgagcaa attttattgc
     4801 tggcgtttgg tcgcaaagcc gctgaagaga tggacgagcg tattcgagaa cgactacata
     4861 ccgaagacat taccgcacgc acatttcatg cgctggcgct gcatattatt cagcagggca
     4921 gcaaaaaagt tccgatagtc agcaaactgg aaaatgatac cgctgcccgt catgaacttt
     4981 ttattgctga gtggtgcaag caatgcagcg aaaagaaagc gcaggcgaag ggctggcggc
     5041 aatggctgac ggaagaaatg cagtggtcag tgccagaagg taacttctgg gatgatgaaa
     5101 aattacagcg tcgtcttgcc tcacgcctcg atcgttgggt aagtctgatg cggatgcacg
     5161 gtggtgcaca ggcagaaatg attgccagtg cacccgaaga gattcgcgat ctgttcagta
     5221 aacgtatcaa gttgatggct ccgttattaa aagcctggaa aggtgcgctg aaggcagaaa
     5281 acgctgtcga tttttcgggc cttattcacc aggcgattgt gattctggag aaaggtcgct
     5341 ttatcagccc gtggaagcat attctggttg atgaatttca ggatatctcg ccgcagcgcg
     5401 cagcgttgtt agcggcatta cgcaagcaaa acagtcagac gacgttgttc gccgttggtg
     5461 atgactggca ggcgatttat cgcttcagcg gtgcgcaaat gtcgctcacc accgctttcc
     5521 atgaaaactt tggtgaaggt gatcgctgcg atttagagac gacttaccgt tttaacagtc
     5581 gtatcggtga ggtggcaaac cggtttattc agcagaaccc aggccagctg aaaaagccgc
     5641 taaacagctt aaccaatgga gacaaaaaag ccgtcacgtt attggatgag agccatcttg
     5701 acgctttgct ggataagctc tctggttatg ccaaaccgga agagcgcatt ctgatcctgg
     5761 cgcgttacca tcacatgagg cctgccagcc tggaaaaagc ggcaacacgc tggccgaagt
     5821 tgcaaatcga ctttatgacc attcatgcca gcaaagggca acaggcggat tacgtcatca
     5881 tcgttggctt gcaggaggga agtgatggtt ttccggctgc ggcgcgggag tcgattatgg
     5941 aagaggcgct actgccaccg gttgaggatt tcccggacgc tgaagaacgg cggttaatgt
     6001 acgtggcgct gacccgggca cgccatcggg tatgggcact gtttaacaaa gagaatccct
     6061 ctccctttgt ggaaatactg aaaaatctgg atgtgccggt ggcgagaaaa ccgtaagaaa
     6121 caggtggcgt ttgccacctg tgcaatatta cttcagacgg tccgcgagat aacgctgata
     6181 atcggggatc agaatatcga ccgcgtcgtt gaaatgcggc gactggatta tgaagtctgc
     6241 cgttgccacg ttggtggcga ccggaatgtt ccataccgtc gccagacgca gcaaggcttt
     6301 cacgtcagga tcgtgcggca cggcatttag tggatcccag aagaaaatca atacatcaat
     6361 tttcccttct gagatcaatg cgccaacctg ctggtcaccc cccattgggc cactcaacat
     6421 cgcgttgacg ttcatgccgg tcgcgcggga aattaagtta ccggtagtgc ctgttgcata
     6481 cagtacgtgt tgttccagta acggttgatg ccgttccacc cagctcatca gcatttgttt
     6541 gcagtgatcg tgtgccacca gcgcaatatg tttccgcgca ggtaaagtgc gagtcgtcag
     6601 ttccataatg tacatccgta gttaactttc ctacagatta ctgtaagcac ttatcgctgc
     6661 aagataaaga ccgaaaaagc ctgcgcacag gcacaaaaat ctcaggaaga tggttgtttt
     6721 tccgcccatt gcaggaaagt atttcgcgtt tgtgggtcag ccagtttaaa ccaatacttc
     6781 agccgttgtt ctgtgagcac ctgagactgc ggtggaatag catccagctc ggaaaccccc
     6841 gaaagcagcg tactgtcatc tgccatcatc gtggcaaatt gcggtagtga agcgcgtttc
     6901 gcggatttgt tatagcgttc aacctcgacc tcataatcaa tcgtttttgc cgttgaggta
     6961 atggcgagaa tatccagctt taccggaatc ggcgtcgcat cgccatccaa caattcaagg
     7021 cgcggcgcag catcaaaatg gttagcttcc cgctcattct ccagacgagg caggcgaaaa
     7081 ttgacctggt tgatgagctg ggtattaaaa ctgaccacca gcggtgggga gatatacagc
     7141 cgttcttcac tattggaaag atgaatcgtc ttctcaacgc gaaacactaa ctgatgcggt
     7201 ccgttatcca gttcaatgct gtccgcaccg cgcagcagag agctggaaac ctttttaccg
     7261 tcgagtacca gaagatcgac atcggttgaa agccgcagcg tggtggcaaa aacggatacc
     7321 ggcaaacata atgcaatcaa ggtggtcacg atgccggttt tcataggtag ctcctgtcaa
     7381 aagaccgccc gaataataat gtgtcaaaat ttttctttaa tcatacttac taacatatag
     7441 acatatttcc cgcttttgct ctcattcatt cgtattagct gcatggttgg catgtcggct
     7501 ttgtcgtaca ctttgttaaa cagtctggag gaaataatga aagaaaccga tattgcaggc
     7561 attttaacgt ccactcacac cattgcgctg gtgggggcaa gcgacaaacc cgatcgtccc
     7621 agctatcgcg tgatgaaata tctactcgac caggggtatc acgtcattcc ggtttcgcca
     7681 aaagttgctg gcaaaacact gctgggccag caggggtatg gcacgctggc agaagttcct
     7741 gaaaaagtcg atatggtcga tgttttccgg aattcagaag cagcgtgggg cgtagcgcag
     7801 gaagcgatcg ccatcggcgc gaaaaccctc tggatgcaac tgggggtgat taatgagcaa
     7861 gcggcagtac tggcacggga tgccggatta aatgtagtga tggatcgctg cccggctatt
     7921 gagatccctc gcctgggcct ggccaaataa aaaatccccg gaaggcaaaa accttccggg
     7981 gatttgttca gggattagtt acgcagacgc ggggcctgga gttgtttgcg gatggtctgc
     8041 gccagctcat ccatggatgg ctgttcagga tgttcatcct gcaattcgct acttagctgg
     8101 gcttcagcca ggtaggtatg aaccggtagg ccgttatcgt cctccatcac cacgtgatac
     8161 cacggagcgg cgcgaagctc gtcattcacc gccagctcat caggcgacgg ttcagagagc
     8221 gaataaaccg ggtcgatatc cacgaccact ccgagataac ctaacaggga atggcggacc
     8281 tgctggccga taccgaattt gctggcaatc atagtcacct cccgggaaat ctttaccttt
     8341 gatataaggg taaaattcca catttcaagt tacatgacgc gacaggcaaa ccctttcaga
     8401 tatagccctt ccggataggt agcgatcacc ggatgatcgg ctgcctgacg gaactgctct
     8461 ataaattgta catcacgacc ggcatcaatt gcggcatccg cgatgatttt ctgaaataaa
     8521 tcgctggtca tcaggccaga acaggagaaa gtcaggagaa ctccgccttc attcagcaac
     8581 tgaatcgcca gcatgttgat gtctttatag ccacgacacg cgcccatcaa ctggctttta
     8641 ttctcaacaa acttcggcgg gtccatcacg ataacgtcaa atttttcacc gcgatcgcga
     8701 taagtacgca gcaatttaaa gacatcatca cggacaaact cagccttgct cagatccagt
     8761 ttgttcagct caacgttctg ccgtgcaata tccagcgctt cctgggaggt atcaacgctg
     8821 acaacctggc tgcaaccgcc catcagtgcc gatacggcga aaccaccggt ataggagaaa
     8881 cagttcagca cacgtttatt ttcaacgtag cggcgggtag ccaggcggct gtcacgctgg
     8941 tccaggtagt agcccgtttt gtgcccgtgc tgtatgtcca ccagcagctt catgccgtgt
     9001 tcttcaatcg gcagcagggc aggcggtagc tctccggtaa cggggccctg ggtcagttcc
     9061 atcccttctt ttttacgtac cgcaacgtcg ctgcgatcgt aaatcgcaca ttccgggtac
     9121 agcgtttgca gggcactaat taatgctgcg cgctgatatt ctgcgccagc actcagcaat
     9181 tgcagcacca gaaaattacc gaaacgatcg atagtaatac ccggcaggcc atcagattca
     9241 ccggcgatta aacgatagct gtcgaggcca tctttttgcg ccagccagtc acgccatttt
     9301 tgtgcttgtt gcaaacggcg ggaaaaaaaa gcaatgtcga tagattcatt cgggtcaaac
     9361 gtccagacgc gcgcccggat ttgcgaagct ggcgaataag cgccgcgtgc taaccatttt
     9421 ccctgatgat caacaatatc gatggtttcc ccgaggctgg ctttaccttc catgcgggca
     9481 acggccccgg aaaagatcca cggatggcga cgaagtaatg atttttcgcg ccctttggct
     9541 aacactaaac gtacactcat aatttacttt tctgtcgatg caaagaaatg ggcgtcattg
     9601 tccggagttt tccatggatt tgcaacgcgt ccattaagga taaggagagc agcaatgtcg
     9661 aaagtctgca taattgcctg ggtttacggg cgagttcagg gcgttggatt tcgctatacc
     9721 acgcagtacg aagcgaaaaa actgggatta gccgggtacg ccaaaaatct tgacgatggc
     9781 agcgtggaag tggttgcctg cggtgaagaa gggcaggtag aaaaattaat gcagtggtta
     9841 aagagtggcg gcccgcgttc tgcgcgtgta gaacgggtgc tcagcgagcc gcatcatccc
     9901 tcggaggaat taaccgattt tcgtatccgt taaatacatt taaccggttt tggcaggcca
     9961 gcaattttgg tggcttgctt agccggacct ttcggaaaca gtcggtataa atagcggctg
    10021 ttaccttttt cttcgccaaa tttattcgcc atcgctttta ccagcatacg aatcgccgga
    10081 gaagtattga attccagata gaaatcacgc acaaaacgca ccacttccca gtgttctggc
    10141 gacagcgaaa tcccttcgtt ctctgcaatc accactgcca gcggctcact ccactggctg
    10201 ctttctttga gatagccttc ggtatccgtt tctatctctt taccttcgaa gatcagcata
    10261 attattacta ccttaatcag actggcggca gtgtaacaaa aaacaaagcc ccgcataaag
    10321 cgaggctcgg taagtaaccc tgggcgagat taatcgcggc tggcgaagcc cagaatgctc
    10381 agcaggctga cgaagatgtt gtacagcgaa acatacaggc taaccgtggc acgaatatag
    10441 ttcgtttcac cgccatgaat gatgttgctg gtttcaaaca aaatagcgcc agaggagatc
    10501 agaatgaaga ccgcgctgat cgccagatgc agagcaggca gttgcaggaa gatattagca
    10561 accataccaa tcagcaccac cacaataccc gccatcagca taccgccgag gaacgacata
    10621 tctttgcggg tggtcagcac atatgcagag cagcagaaga acactaacgc cgttccgccc
    10681 agtgccatag cgattacgtc acccattccg gcagacagat aggtattcag aattggtccg
    10741 aggatataac ccagaaaacc ggtaaaggcg aatgcggaga taatcccggt cggcttattc
    10801 gccgttttat aggtcaggaa catcaaacca tacataccca ccagcgtcag aatcagaccc
    10861 ggagatggca gcatcagcac cgtactggca gttgcggtaa tcgcagaaaa ggccagcgtc
    10921 aggctcagca gaaaataggt attacgcagc accttatggg tgctaagcag cgatgtacgg
    10981 tcatgtgaag aactaacaat acgatccatt gagtcactct ctatgacaga tgtaattaat
    11041 taagcagcat aatgataatg cgtaagggca cccagaagtt ttacccatct ttacgcattt
    11101 gatctggaac aggtttaaca gcggattatc aggtcattaa gcaaatataa cgccctgaga
    11161 atttcgacag gcaaaagaaa aaggggttag catttagcta accccttatc ttatttggcg
    11221 gaagcgcaga gattcgaact ctggaaccct ttcgggtcgc cggttttcaa gaccggtgcc
    11281 ttcaaccgct cggccacact tccggaatga cgcgcactat aaacatcccg atgcggcgtg
    11341 taaaccccta atttgtttgt ttgcctgaaa aacagacaaa agtgcattga tagcgtgaaa
    11401 taacagcaga ttgatcattt catcaccatg aattccttct cttttactcg tttagcaacc
    11461 ggctaaacat ccccactgcc cggcaaaaag aaaaataggt ccatttttat cgctaaaaga
    11521 taaatccata cagtttgtat tgttttgtgc aaaagtttca ctacgcttta ttaacaatac
    11581 tttctggcga cgtgcgccag tgcagaagga tgagctttcg ttttcagcat ctcacgtgaa
    11641 gcgatggttt gccttgctac agggacgtcg cttgccgacc ataagcgccc ggtgtcctgc
    11701 cggtgtcgca aggagaagag acgtgcgata tgaataacga ggaaacattt taccaggcca
    11761 tgcggcgtca gggcgttacc cggcgcagct ttctcaaata ttgtagtctg gctgccacgt
    11821 cgctgggctt aggcgcggga atggcaccaa agattgcctg ggcgctggag aacaaaccgc
    11881 gcattccggt ggtatggatc cacggtctgg aatgcacctg ctgtaccgaa tcttttatcc
    11941 gctccgctca cccgctggcg aaggacgtca tcctttccct gatttccctc gattatgacg
    12001 atactttgat ggctgccgcc ggaacccagg cggaagaagt ctttgaagac atcatcacgc
    12061 aatacaatgg caaatatatc ctcgcagtag aaggtaatcc gccgctgggc gagcagggga
    12121 tgttctgtat cagcagcgga cgaccgttta ttgagaaact caaacgtgcc gctgccggag
    12181 ccagtgcgat tatcgcctgg ggaacctgcg cgtcctgggg ctgcgtgcag gccgcgcgac
    12241 ccaatccgac gcaggcaacg cctatcgaca aagtcatcac cgacaaacct attatcaaag
    12301 tacctggctg cccgccgatc ccggatgtga tgagcgccat cattacttac atggtgacct
    12361 ttgatcgctt gccagatgtc gacagaatgg gccgtccact gatgttttat ggtcagcgta
    12421 tccacgataa atgttatcgc cgcgctcact tcgacgccgg agagttcgtc cagagttggg
    12481 atgatgacgc tgcccgcaaa ggttactgcc tgtacaaaat gggctgcaaa gggcctacca
    12541 cctataacgc ctgttcctcc acacgctgga acgatggcgt ttccttcccg atccagtcag
    12601 gtcacggctg cctgggttgt gcggaaaatg gtttctggga tcgcggttcg ttctacagcc
    12661 gcgtggtcga tattccacaa atgggtactc attccaccgc cgacaccgtc ggtttaaccg
    12721 cgcttggcgt ggtggcagcg gctgttggtg tgcacgcagt cgccagcgcc gttgaccagc
    12781 gcagacgtca taaccagcaa cctacagaaa ccgaacatca gccaggcaat gaggataaac
    12841 aggcatgagc actcagtacg aaactcaggg atacaccatc aataatgccg gacgccgcct
    12901 ggtggtcgac ccgattacgc gcattgaagg ccacatgcgc tgcgaagtga atattaacga
    12961 tcagaatgtg atcaccaatg ccgtctcctg cggcaccatg tttcgcgggc tggagatcat
    13021 cctgcaaggg cgtgacccgc gcgatgcgtg ggcgttcgtt gaacgtatct gcggcgtctg
    13081 taccggcgta cacgctctgg cttcggtcta tgccatcgaa gatgctatcg gcattaaagt
    13141 gccggacaac gctaatatca tccgcaacat tatgctggcg acgctctggt gccatgacca
    13201 tctggtgcac ttttatcagc ttgccgggat ggactggatc gatgtgttag atgcgctgaa
    13261 agccgacccg cggaaaacct ccgaactggc gcaaagtctc tcctcttggc cgaaatcatc
    13321 ccctggctat ttcttcgacg tacaaaatcg cctgaagaaa ttcgttgaag gcggccagtt
    13381 ggggatcttc cgcaatggct actgggggca cccgcagtac aaactgccgc cagaagccaa
    13441 cctgatgggc tttgcccact atctcgaagc tctcgatttc cagcgtgaaa ttgtcaaaat
    13501 ccacgctgtc tttggcggta aaaacccgca tccaaactgg attgtcggcg ggatgccttg
    13561 cgccattaac attgacgaaa gcggcgcggt cggggcggtc aatatggaac gcctgaacct
    13621 ggtgcagtca atcattaccc gcacggcgga tttcattaac aacgtgatga tccccgacgc
    13681 cttagccatt ggtcagttca acaaaccgtg gagcgaaatc ggcacgggtc tttctgataa
    13741 atgcgttctc agttacggcg cgttcccgga tatcgccaac gactttggcg agaaaagtct
    13801 gctgatgcct ggcggcgcgg tgattaacgg cgacttcaac aatgtgctgc cagtggattt
    13861 ggttgatccg cagcaggtgc aggagtttgt cgaccacgcc tggtatcgat atcccaacga
    13921 tcaggtcggg cgtcatccgt tcgatggcat caccgacccg tggtacaacc ccggcgatgt
    13981 caaaggcagc gataccaaca ttcagcagct gaatgaacag gaacgctact cgtggattaa
    14041 agcgccacgc tggcgcggta acgcgatgga agtggggccg ctggcacgca cgttaatcgc
    14101 ttatcacaaa ggcgatgctg cgaccgttga gtcggtcgat cgcatgatgt cggcgttgaa
    14161 actgccgctt tccggcatcc agtcaacgtt gggccgcatt ttgtgtcgcg cgcacgaagc
    14221 gcagtgggcc gcaggtaagt tgcagtattt cttcgataag ctgatgacca atctgaaaaa
    14281 cggcaatctc gccactgctt ccactgaaaa atgggaacct acaacctggc cgacagagtg
    14341 ccgtggcgtc ggctttaccg aagcgccgcg cggggcgtta ggccactggg ctgccattcg
    14401 cgatggcaag attgatctct atcagtgcgt ggtgccgacc acctggaacg ccagcccgcg
    14461 cgatcctaaa gggcaaattg gcgcttatga agcggcgctg atgaacacca aaatggcgat
    14521 ccccgagcaa ccgctggaga tcctgcgtac tctgcacagc tttgatccgt gcctcgcctg
    14581 ttcaacacac gtgctgggcg acgacggtag cgagctggtc tccgtgcagg tgcgttaaca
    14641 gcgaaggaga atcatcatgc aacagaaaag cgacaacgtt gtcagtcact atgtctttga
    14701 agcacccgtg cggatatggc actggctgac ggtgttatgc atggcggtgt tgatggtcac
    14761 cggttacttt atcggcaagc cgctaccttc cgtcagcggc gaggcgacgt atctgttcta
    14821 tatgggctac atcaggttaa ttcacttcag cgccgggatg atttttaccg tggtcttgct
    14881 gatgcggatc tactgggctt ttgttggcaa tcgttactcc cgcgagctgt ttatcgtgcc
    14941 ggtatggcgt aaaagctggt ggcagggcgt gtggtatgaa atccgctggt atctgtttct
    15001 ggcaaaacgt ccgagtgccg atataggcca taaccccatc gcccaggcgg cgatgttcgg
    15061 ctatttcctg atgtcagttt ttatgatcat caccggcttt gcgctgtaca gcgaacacag
    15121 ccagtacgct atttttgcgc cgttccgtta tgtggtggaa tttttctact ggacgggcgg
    15181 caactcaatg gacgttcaca gctggcatcg gctggggatg tggctgattg gcgcgtttgt
    15241 gatcggtcat gtctacatgg cgctgcgtga agatatcatg tccgacgaca cggtgatctc
    15301 caccatggtc aacggctacc gtagccacaa atttggcaaa ataagtaaca aggagcgttc
    15361 atgagcgagc aacgcgtggt ggtaatgggg ctgggcaacc tgctgtgggc cgatgaaggc
    15421 ttcggcgtgc gggtggcgga acggctgtat gcccattacc actggcccga gtatgtggag
    15481 attgtcgatg gcggtactca gggactgaac ttgctggggt atgtcgaaag cgccagtcac
    15541 ttgttgatcc tcgatgccat tgactacggg ctggaaccag gaacgctgcg aacctatgcc
    15601 ggagaacgca ttccggctta tctcagcgcg aagaaaataa gcctgcacca gaacagcttc
    15661 tccgaagtgc tggcgctggc ggatatacgt ggtcatttgc cagcacatat tgccctcgtc
    15721 ggtctgcaac ccgcaatgct cgacgactac ggcggtagcc tgagcgaact ggcgcgggag
    15781 caactgcccg ctgcggaaca atcggcgctg gcgcagctgg ctgcgtgggg aattgtgccg
    15841 caaccggcta atgaatcgcg ctgtctcaat tatgactgtc tgtcgatgga aaattacgaa
    15901 ggcgttcgct tgcgtcagta caggatgagg ctggaggaac agggatgagc aacgacacac
    15961 catttaatgc gttgtggcaa cgaatgctgg cgcgtggctg gacgccgatc agtgaatgcc
    16021 gtctcgacga ctggcttacg caagcgccag acggcgtggt gttattaagc agtgacccga
    16081 aacgcacgcc agaagtcagc gataacccgg tcatgattgg cgaattactg cgcgagtttc
    16141 ccgactatac atggcaggta gcgattgctg accttgagca gagcgaagcc atcggcgatc
    16201 gctttggcgt ctttcgcttt cccgccacat tagtatttac cggcggaaac tatcgcggcg
    16261 tgctgaatgg tattcacccg tgggcggaac tgataaacct gatgcgcgga cttgtcgaac
    16321 cgcagcagga gcgtgcctca tgagcgaaac ttttttccat ctgctggggc caggaacgca
    16381 accgaacgat gacagtttca gcatgaatcc actgccgatc acctgtcagg tgaatgatga
    16441 accgagtatg gcggccctgg agcaatgtgc tcacagcccg caggtgattg cgctattaaa
    16501 cgagttacaa catcaactaa gcgagcgcca gccgccgctg ggcgaggtgc tggcagtcga
    16561 tctgttaaat ctcaacgccg acgatcgtca ctttatcaat acgcttctcg gggaagggga
    16621 agtgtcagtg cgcattcagc aggctgacga cagtgaaagt gaaatacagg aggcgatctt
    16681 ctgcggatta tggcgggtgc gcagacgtcg cggcgacaag ttgctggagg acaaactgga
    16741 ggctggctgc gcgccgctgg tattgtggca ggcggcaacg caaaacgtct tgccgacaga
    16801 ttcgctgtta ccgccgccca ttgatggcct gatgaatggc ctaccgttgg cgcatgagtt
    16861 actggcgcat gtacgtaacc ccgacgcgca gccgcacagc attaatctga cgcaattacc
    16921 catcagcgag gctgatcggc tttttctctc acgtctctgt gggccgggaa atattcagat
    16981 tcgtaccatt ggctatggcg agagctatat caacgccacg gggttacgcc atgtctggca
    17041 tttacgctgt acggacacct taaaaggccc gttactggaa agttatgaaa tctgcccaat
    17101 accggaagtg gtgctggcag cgccagaaga tttggtcgac tctgcgcagc ggcttagtga
    17161 ggtatgtcag tggctggcgg aaggggcacc gtcgtaaaaa gacggtaagt atagctttca
    17221 ggcttatgaa tatcgcaatc ggcgaatacc tctggtcgta gagtttcagg ataaagaggg
    17281 agatctacca ttaacgggtt atttttctct cttcgcctac aggagtgcgc atgtgggatg
    17341 tcattgattt atcgcgctgg cagtttgctc tgaccgcgct gtatcacttt ttatttgtac
    17401 cccttaccct ggggctgatt tttttgctgg ctatcatgga aaccatttac gtggtcaccg
    17461 gcaaaacaat ctaccgcgat atgacgcgct tctggggtaa gctcttcggt atcaactttg
    17521 ctcttggcgt ggctaccggc ctgacgatgg agtttcagtt tggtactaac tggtcattct
    17581 attccaacta tgtgggcgat atttttggcg caccgctggc aatggaagca ttaatggcct
    17641 tcttcctcga gtccaccttt gtcgggctgt tcttcttcgg ctggcagcgg ctgaataaat
    17701 accagcacct gctggtgacg tggctggtgg cgttcggttc aaatctctct gcgttgtgga
    17761 tattgaatgc caacggttgg atgcaatacc cgaccggtgc gcattttgat atcgacaccc
    17821 ttcgtatgga aatgaccagc ttcagcgagc tggtctttaa cccggtcagc caggtgaaat
    17881 ttgtgcacac cgtaatggcg ggctacgtga ccggggccat gtttattatg gcgatcagcg
    17941 cctggtattt actgcgcgga cgggagcgcg atgtcgcatt acgctcgttt gccatcggtt
    18001 ccgtcttcgg tactctggcg attatcggta cgctgcaact cggagacagt tcggcgtatg
    18061 aagtcgcgca agtacaaccg gttaaactgg cggcaatgga aggggagtgg caaacggaac
    18121 ctgcacctgc accgttccat gtggttgcct ggccggaaca ggatcaagag cgtaacgcct
    18181 ttgccatcaa aattcccgcg ctgctaggga tcctcgccac tcactcatta gataaacccg
    18241 tgccgggtct gaagaatttg atggctgaaa cctacccacg cttgcaacgc ggacgtatgg
    18301 cctggctgtt aatgcaggaa atatcgcagg gcaatcgtga gccgcatgtg ttgcaggcat
    18361 tccgggagct ggaaggcgac cttggttacg gcatgttgct ctcccgctat gcgccggata
    18421 tgaatcatgt cacagccgca cagtaccagg tggcgatgcg tggcgcgata cctcaggttg
    18481 cgccggtatt ctggagtttc cgcatcatgg tgggctgtgg ttccctgctg ctactggtga
    18541 tgctgattgc gcttgtccag acgctgcgtg gcaaaatcga ccagcatcgc tgggtgctga
    18601 aaatggcgct ctggagtttg ccgctgccgt ggattgcgat tgaagccggg tggtttatga
    18661 ccgagtttgg tcgtcagccg tgggcgatac aggacatctt accgacatac tccgcgcact
    18721 ccgccttaac cacaggacaa ctggctttct cactgatcat gatcgtaggg ctttacaccc
    18781 tgttcttaat cgccgaagtc tacctgatgc agaaatatgc ccgtctgggg ccgagcgcga
    18841 tgcagagtga acaaccgacg cagcaacagg ggtaaaggag aaaatcatgt ttgattatga
    18901 aacattgcgc ttcatctggt ggctgctgat tggcgtgatc ctggtagtct ttatgatctc
    18961 cgatggattt gacatgggga tcggctgtct gctgccgctg gtggcgcgta atgatgatga
    19021 acgtcggata gtgataaaca gcgttggcgc gcactgggaa ggcaatcagg tctggttaat
    19081 cctcgcaggt ggggcattat ttgccgcctg gcccagagtg tatgcagcgg cgttttccgg
    19141 cttttatgtg gcgatgatcc tggtgctgtg ctcgctgttc ttccgcccgc tggcctttga
    19201 ttatcgcgga aaaattgccg atgcacgctg gcgtaaaatg tgggatgccg gtctggtcat
    19261 cggcagtctg gtgccgccgg tagtcttcgg tattgccttc ggcaacttgc tgctcggcgt
    19321 gccgtttgcc ttcacaccgc aattacgcgt ggaatatctc ggcagctttt ggcaactgct
    19381 gacgccattc cctttattgt gcggattgct cagccttggg atggtgattt tgcaaggtgg
    19441 cgtctggtta caactaaaaa ctgttggtgc gattcatctg cgttcacagc tggcgaccaa
    19501 acgcgccgca ctgctggtga tgctctgctt tttgctggcg ggttactggc tgtgggtcgg
    19561 tattgatggc tttgtactgc tcgcccagga tgctaacggt ccttccaatc cgttaatgaa
    19621 actggtggca gtgctacctg gtgcctggat gaataatttc gtcgaatcgc ccgttctgtg
    19681 gatcttcccg ctgctggggt tcttctgccc attgctgacg gtgatggcga tttatcgtgg
    19741 tcgcccgggt tggggatttt tgatggcgtc attgatgcaa tttggcgtga ttttcacggc
    19801 aggcatcacg ctgttcccct ttgtcatgcc gtcaagcgtg agtccgatct ccagcctgac
    19861 gttgtgggac agtacttcca gtcagctgac gctgagcatc atgttggtaa tcgtgctgat
    19921 atttttgccc attgtgttgc tctacactct ctggagctac tacaaaatgt gggggcgcat
    19981 gacaacagaa actctccgcc gtaacgaaaa cgagctgtac taaggagcag gaacaatgtg
    20041 gtatttactt tggttcgtcg gcattttgtt gatgtgttcg ctctccaccc ttgtgttggt
    20101 atggctggac ccgcgattga aaagttaacg aacgtaagtc tgatgcggcg cattagcgtc
    20161 gtatcaggca atcaataatg tcggatatga aaagcggaaa catatcgatg aaagcgatct
    20221 tgatcccatt tttatctctt ctgattccgt taaccccgca atctgcattc gctcatagtg
    20281 agccggagct gaagctggaa agtgtggtga ttgtcagtcg tcatggtgtg cgtgcaccaa
    20341 ccaaggccac gcaactgatg caggatgtca ccccagacgc atggccaacc tggccggtaa
    20401 aactgggttg gctgacaccg cgcggtggtg agctgatcgc ctatctcgga cattaccaac
    20461 gccagcgtct ggtagccgac ggattgctga cgaaaaaggg ctgcccgcag cctggtcagg
    20521 tcgcgattat tgctgatgtc gacgagcgta cccgtaaaac aggcgaagcc ttcgccgccg
    20581 ggctggcacc tgactgtgca ataaccgtac atacccaggc agatacgtcc agtcccgatc
    20641 cgttatttaa tcctctaaaa actggcgttt gccaactgga taacgcgaat gtgactgacg
    20701 cgatcctcag cagggcagga gggtcaattg ctgactttac cgggcatcgg caaacggcgt
    20761 ttcgtgaact ggaacgggtg cttaatttcc cgcaatcaaa cttgtgcctt aaccgtgaga
    20821 aacaggacga aagctgttca ttaacgcagg cattaccatc ggaactcaag gtgagcgccg
    20881 acaatgtctc attaaccggt gcggtaagcc tcgcatcaat gctgacggag atatttctcc
    20941 tgcaacaggc acaaggaatg ccggagccgg ggtggggaag gatcactgat tcacaccagt
    21001 ggaacacctt gctaagtttg cataacgcgc aattttattt actacaacgc acgccagagg
    21061 ttgcccgcag tcgcgccacc ccgttattgg atttgatcat ggcagcgttg acgccccatc
    21121 caccgcaaaa acaggcgtat ggtgtgacat tacccacttc agtgctgttt attgccggac
    21181 acgatactaa tctggcaaat ctcggcggcg cactggagct caactggaca cttccaagtc
    21241 agccggataa cacgccgcca gggggtgaac tggtgtttga acgctggcgt cggctcagcg
    21301 ataacagcca gtggattcag gtttcgctgg tcttccagac tttacagcag atgcgtgata
    21361 aaacgccgct gtcattaaat acgccgcccg gagaggtgaa actgaccctg gcaggatgtg
    21421 aagagcgaaa tgcgcagggc atgtgttcgt tggccggttt tacgcaaatc gtgaatgaag
    21481 cacgcatacc agcgtgcgct ctccatcaag acaaataaac atttgccgct gttggtcctc
    21541 gcaggccatt tacgcggcaa aattccacgc gtaatcctgg tataagcact tctgcgtcgc
    21601 ggggagtgaa tgcggaaata tggacctgga cttctttacg accgtcggag gggataatga
    21661 atcctttgcc gcttttgcga tcaaaggttt tgacaattcc tgtcatttta cgggacaaac
    21721 aaattcctta ctgaatatac tgcgctgcac tatacggggt taataaaata aagccagcga
    21781 tatttaagac cgccggacgg ctaaaataaa atttgcttaa tctcaattat catgcgttaa
    21841 tagctgcgtc ggtttgaaag acagacagca tacaaagtag tttactaaag cagttctcat
    21901 tatcaggcat tatccccttc ttttgagtct ctctcctgaa cactaagtag tttctgtatt
    21961 aaagccctgt ttgccgaaag gcccaaaatg aaggaagtaa aa
//
LOCUS       DACBVI010000067        21874 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.067, whole genome shotgun sequence.
ACCESSION   DACBVI010000067 DACBVI010000000
VERSION     DACBVI010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.067"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            21..722
                     /locus_tag="IU325_12495"
     CDS             21..722
                     /locus_tag="IU325_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAP5809839.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSTWAAGKGGVGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGP
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLAEAPAKLR
                     CTSLADMVMVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFPLPKGAQNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            747..3347
                     /locus_tag="IU325_12500"
     CDS             747..3347
                     /locus_tag="IU325_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HAP5809840.1"
                     /translation="MPRTHRQHSLLTSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFERGQKITPGVYRVDIVLNQTMVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACAPLAEIIPDASVTFDVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSSDSDSTDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSINSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNDQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMLGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGDYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHTTQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDSGSQQQLNY
                     ALSGSLVAHSRGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIVLDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGVQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINA
                     AITRMDAICR"
     gene            3338..4408
                     /locus_tag="IU325_12505"
     CDS             3338..4408
                     /locus_tag="IU325_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP5809841.1"
                     /translation="MQIIYGEKCVSLLRLFFAAILMLWCAQTMAYTGQCHTAQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDSWYKINDYLAARVLLQVKGNSPTAVPFENVGTGGDTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFIGSVVIPPMTLARLFECYNIPAGDSCTSSGDPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVKVNLSL
                     TATSDPSYPQAIKTSRPGVGIVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            4420..4962
                     /locus_tag="IU325_12510"
     CDS             4420..4962
                     /locus_tag="IU325_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP5809842.1"
                     /translation="MKKKTIFQYVILFFSFLNTHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4970..5485
                     /locus_tag="IU325_12515"
     CDS             4970..5485
                     /locus_tag="IU325_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP5809843.1"
                     /translation="MLKRIIWILCLLGLTGSCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGMQSEMLSI
                     ESGTDAASGLAIAILDDVKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5451..6188
                     /locus_tag="IU325_12520"
     CDS             5451..6188
                     /locus_tag="IU325_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HAP5809844.1"
                     /translation="MPARHLYFIMTNTWNRLTLSIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISISLTNTSQDSWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVSWRTINDYGGLSAKKEQNLL"
     gene            6299..7309
                     /gene="pyrD"
                     /locus_tag="IU325_12525"
     CDS             6299..7309
                     /gene="pyrD"
                     /locus_tag="IU325_12525"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HAP5809845.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSQELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7483..8025
                     /gene="zapC"
                     /locus_tag="IU325_12530"
     CDS             7483..8025
                     /gene="zapC"
                     /locus_tag="IU325_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HAP5809846.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8022..9131)
                     /gene="ycbX"
                     /locus_tag="IU325_12535"
     CDS             complement(8022..9131)
                     /gene="ycbX"
                     /locus_tag="IU325_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HAP5809847.1"
                     /translation="MATLTRLFVHPVKSMRGIGLTHALADISGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            9375..11483
                     /gene="rlmKL"
                     /locus_tag="IU325_12540"
     CDS             9375..11483
                     /gene="rlmKL"
                     /locus_tag="IU325_12540"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HAP5809848.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11495..13402
                     /locus_tag="IU325_12545"
     CDS             11495..13402
                     /locus_tag="IU325_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP5809849.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKIEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            13532..14785
                     /gene="pqiA"
                     /locus_tag="IU325_12550"
     CDS             13532..14785
                     /gene="pqiA"
                     /locus_tag="IU325_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HAP5809850.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14790..16430
                     /gene="pqiB"
                     /locus_tag="IU325_12555"
     CDS             14790..16430
                     /gene="pqiB"
                     /locus_tag="IU325_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HAP5809851.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16427..16990
                     /gene="pqiC"
                     /locus_tag="IU325_12560"
     CDS             16427..16990
                     /gene="pqiC"
                     /locus_tag="IU325_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HAP5809852.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17246..17413
                     /locus_tag="IU325_12565"
     CDS             17246..17413
                     /locus_tag="IU325_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HAP5809853.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17483..18001)
                     /gene="fabA"
                     /locus_tag="IU325_12570"
     CDS             complement(17483..18001)
                     /gene="fabA"
                     /locus_tag="IU325_12570"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HAP5809854.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18070..19830)
                     /locus_tag="IU325_12575"
     CDS             complement(18070..19830)
                     /locus_tag="IU325_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP5809855.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARALQSDAGQLVGGHYEVSGHTIRLR
                     NAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTLTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLPSELLKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20016..20468
                     /gene="matP"
                     /locus_tag="IU325_12580"
     CDS             20016..20468
                     /gene="matP"
                     /locus_tag="IU325_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HAP5809856.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20543..21595)
                     /gene="ompA"
                     /locus_tag="IU325_12585"
     CDS             complement(20543..21595)
                     /gene="ompA"
                     /locus_tag="IU325_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HAP5809857.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFTFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQG
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
BASE COUNT         5583 a         5188 c         5738 g         5365 t
ORIGIN      
        1 ttttttaaga gtgactattt atgaaaactt gcataacaaa gggaattgtg accgtaagtt
       61 taacggcaat attactcagc tgttcgtcca catgggcggc aggtaaaggc ggggttggac
      121 ttgcagcaac acgtcttgtt tattcggaag gtgaagagca aatttcactg ggtgtacgta
      181 acaccagtcc ggatgttcct tatctgattc agtcatgggt gatgacacct gataataaaa
      241 agtcagcgga ctttattatt acaccaccgc tatttgtgct gaatccggca aacgagaatc
      301 tgttacgcat tatgtatatt ggtccgccgt tggcgaaaga cagagaaact cttttcttca
      361 ccagcgtacg ggcagtccct tcaacaacga agcgggaaga aggcaatacc ctgaagattg
      421 ccacacaaag cgtcatcaaa cttttctggc gaccaaaagg tttagcatat cccttagccg
      481 aggctccggc gaaactgcgt tgcacttcgt tagctgacat ggttatggtc agtaacccaa
      541 caccttattt cattaccctg acagacctga aaataggtgg aaaagtagtt aaaaatcaaa
      601 tgatctcccc ctttgataaa taccaattcc ctctgccaaa gggtgctcaa aacagtagcg
      661 taacgtaccg aaccatcaat gactacgggg cggaaacgcc gcaactcaac tgtaaatcgt
      721 aagccgtctt cagttaagag agcgagatgc ctagaactca ccgacaacac agcctgttaa
      781 cctctggtgg agtgccatcg tttattggtg ggctggtggt gtttgtgtcg gcggcgttca
      841 atgcacaagc tgaaacctgg ttcgatcccg cctttttcaa agatgatccc tcaatggtgg
      901 ccgatttgtc ccgtttcgaa agagggcaaa aaataacgcc aggcgtttat cgtgtcgata
      961 ttgttctgaa tcagacaatg gtagatacgc gcaacgttaa ttttgttgag ataacgccag
     1021 agaaggggat tgccgcctgc ttgactactg aaagcctgga tgcaatgggc gtgaatactg
     1081 atgcgtttcc ggcttttaaa caactggaca aacaagcgtg tgcgccattg gcagagatta
     1141 ttccggatgc cagcgtaacc tttgatgtga ataaactccg tctggaaatt tcagtaccgc
     1201 aaattgctat aaaaagtaac gcccgtggct atgttccccc tgaacgctgg gatgaaggga
     1261 tcaacgcact attactggga tattctttta gcggggccaa cagtattcat agcagttcag
     1321 atagtgattc cacagatagc tattttttga atttaaacag tggcgtgaat ttaggtccgt
     1381 ggcgattgcg caataattcc acgtggagtc gtagtagcgg ccaaaccgca gaatggaaga
     1441 atctcagcag ctatttgcag cgggcggtta ttcctctgaa aggcgaactg accgtcggtg
     1501 atgattatac cgcaggcgat tttttcgaca gcgtcagctt tcgtggcgta cagctggcgt
     1561 cagatgacaa catgctgcca gacagcctga aagggtttgc gcctgtggtg cgtggtatcg
     1621 ccaaaagcaa tgcccagata acgattaagc aaaacggcta caccatttac caaacttatg
     1681 tctcgccagg cgcttttgag attagtgatc tctactccac gtcgtcgagc ggtgatttgc
     1741 tggttgaaat aaaagaagct gacggcagca tcaatagtta cagcgtgccc ttttccagtg
     1801 tgccattact ccagcgtcag gggcgaatca aatacgcggt gacgctggcg aaatacagaa
     1861 ccaatagtaa tgatcagcaa gaaagtaaat ttgctcaggc cacgctgcaa tggggcggac
     1921 cgtggggaac gacctggtat ggcggtggac aatatgctga atattaccgt gccgctatgt
     1981 tggggctggg ttttaacctc ggcgatttcg gagcgatttc gttcgacgcg acccaggcga
     2041 aaagtacact ggcagaccaa agtgaacata aagggcagtc atatcgtttt ctgtatgcca
     2101 aaacgctcaa ccaattgggc actaactttc aattgatggg ctatcgctat tcgacgtcag
     2161 gcttctacac cctttccgac accatgtata aacacatgga tggctacgaa tttaatgacg
     2221 gtgatgatga agatacgccg atgtggtcgc gttattacaa tttgttttac accaaacgtg
     2281 gcaaactgca ggtcaatatc tcccagcaat taggcgatta cggttcgttt tatttaagtg
     2341 gtagccagca aacatactgg cataccgatc aacaggatcg gctattacag tttggctaca
     2401 acacgcaaat taaagatctc tcactgggcg tttcctggaa ctacagtaag tcccgtggtc
     2461 aacctgatgc tgatcaagta tttgcactta atttttccct gccgctcaat ctgttgctcc
     2521 ccagaagtaa tgatagctat accaggaaaa aaaattacgc atggatgacc tctaatacca
     2581 gtatcgataa cgaagggcac actacacaaa atcttggttt aacggagaca ctgctcgatg
     2641 acggtaacct gagctacagc gtgcaacagg gatataacag cgaggggaaa acggcgaatg
     2701 gtagcgccag catggactac aaaggggcgt ttgcggatgc ccgagtgggc tacaactaca
     2761 gcgatagcgg tagccaacaa caacttaact acgccctttc aggcagttta gttgcccatt
     2821 cacggggcat taccctgggg caatcactgg gggaaaccaa cgttctgatt gcagcaccag
     2881 gcgcggaaaa tactcgtgtg gcgaatagca ccgggctgaa aactgactgg cgcggatata
     2941 ccgttgtccc ctatgccact tcttatcgtg aaaatcgaat cgtacttgat gctgcatcgt
     3001 taaaacgtaa cgtggatctt gaaaatgcag tagtaaatgt agttcccacc aaaggggcgt
     3061 tggttctggc ggagttcaat gcccatgcgg gggcgagggt attaatgaaa acatcaaagc
     3121 agggtatacc gctgcgattt ggcgcgatag caacgctgga tggggtacag accaatagcg
     3181 gcataattga tgacgatggc tcgctctata tgtccggttt gcccgctcag ggagcgataa
     3241 ctgtacgctg gggtgaagct cccgatcaaa tttgtcatat cagttaccag cttaccgaac
     3301 aacaaattaa cgctgcgatt acgcgaatgg atgccatatg cagataatct atggagaaaa
     3361 atgcgtgtca ttactacgac tattttttgc cgccatctta atgctatggt gcgctcaaac
     3421 tatggcttat accgggcagt gccataccgc tcaggggaat ccgtatattg gcgtcaattt
     3481 tggcgttaaa accctggatg aagaagaaaa tacggccgga gtagttaaag acaaatttta
     3541 tcagtggaac gaatcgaatg attactatgt ttcttgtgat tgcgataaag acaatgtcag
     3601 gagtggcaga tgggcattcg ctgcggattc accgttagtc tatttaggcg acagctggta
     3661 caaaattaat gactatcttg ccgccagggt tttattacag gttaaaggta attctcctac
     3721 ggcggttcca ttcgaaaacg tgggcaccgg gggggatacc cgatggcata tttgcgatcc
     3781 cggcggtcaa cgtttaggcg gccagggggc tagcggtaat agcggtagct tttccctgaa
     3841 aatattgcag ccgttcattg gctcggtcgt cattcctcct atgacgctgg cacgattgtt
     3901 tgaatgctac aacatacccg caggggattc ctgtacgtcg tcaggcgacc cggttttagt
     3961 gtattacctg tctggcacga tcaattcact tggctcatgt tccgtcaatg ccggagagac
     4021 aatcgaggtc gatctgggcg atgtatttgc ggctaatttt cgtgttgtag ggcataagcc
     4081 tcttggggcc agaacggctg aactcgcaat ccctgtcagg tgtaacactg gaaacgctgg
     4141 gttagttaag gtcaacctga gtctgacggc aacttcagac cccagctatc cccaggcgat
     4201 taagacttca cgtcctggcg tgggcattgt ggtgaccgat agccagaaca acattatttc
     4261 cccggcgggt ggaacactac cgctctctat tcctgatgat gcagacagta tcgcgcgaat
     4321 gaatgtctat ccggttagca cgacaggcgt accaccagaa accgggcgat ttgaagccac
     4381 ggcaacggtg agaataaatt ttgattaacg cgtgaacgta tgaagaaaaa aacaatattt
     4441 cagtacgtta tcttgttctt tagctttctt aacacccatg tcgggatggc tgggcctgaa
     4501 caagtcagta tgcatattta tggtaatgtg gtcgatcagg gctgtgatgt tgccaccaaa
     4561 agcgcattac aaaatattca tattggcgat tttaatatca gtgattttca ggctgcgaat
     4621 accgtaagca cagctgctga tttgaatatc gatatcaccg gttgtgccgc tggtattact
     4681 ggcgcggacg tcctttttag cggcgaggct gacacccttg cgccgacact actcaaacta
     4741 actgacacag gcggaagcgg tggtatggca acggggattg ccgtgcaaat tcttgatgcg
     4801 caaagtcagc aagaaatccc gctcaatcag gtccagcctc ttacgccctt aaaagcgggg
     4861 gataacacac tcaaatatca acttcgttat aagtccacaa aggcgggggc tacgggcggt
     4921 aatgcgacgg cggttctcta ttttgatctg gtttaccagt gaaggagtga tgttgaaacg
     4981 gataatctgg attctgtgct tactgggttt aacggggagt tgtgagctat ttgcccatga
     5041 tggcacggtc aacatcagcg gatcgtttcg ccgtaataca tgcgtgctgg cacaggatag
     5101 caagcaaatc aatgtgcagt taggcgatgt ctcgctgaca cgtttttctc atgggaacta
     5161 tggcccggag aaatctttca tcattaattt gcaggattgc ggaacagatg tcagcacggt
     5221 tgacgttacc ttttcaggaa cacccgacgg aatgcagagc gaaatgctgt ccattgaaag
     5281 tggtacggat gcagccagtg ggctggcaat tgctattctg gatgatgtga aaatattgat
     5341 tccgcttaac caggcgagta aagactacag cctacatagc ggtaaggtgc cattaacttt
     5401 ttatgcgcaa ttgcgacctg ttaatagcga tgtgcagtcg ggtaaagtga atgccagcgc
     5461 gacatttgta cttcattatg actaatacgt ggaacagatt gacgctctcg atttttgccg
     5521 ttttatcgct gctggtggcg ggggaattgc aggcaggagt ggtggttggc ggaacgcgat
     5581 ttatcttccc ggcagacaga gaatcgatat ctatttcact gactaatacc agtcaggatt
     5641 cctggcttat taacagtaaa atcaatcgcc caacgcgttg ggccgggggg gaagcgtcga
     5701 cagtgccagc accattactg gccgctccgc cacttattct cctgaagccc ggtactacag
     5761 gcacgttgcg cttgctgaga acggaaagcg acatcttgcc tgtggatcgc gaaacgctat
     5821 ttgagttaag cattgccagc gtaccatccg gcaaagttga aaatcagagc gtaaaagtgg
     5881 cgatgcgctc ggtatttaaa ctgttctggc gacccgaagg tttgccgggc gacccgctgg
     5941 aagcttatca acaattacgc tggacacgga attcgcaggg tgtacaactc actaacccaa
     6001 cgccttacta cattaacctg attcaggtga gcgtaaatgg taaagcgtta agtaatgcgg
     6061 gagttgtgcc acctaaaagc cagcgtcaga caagctggtg tcaggcaata gcaccgtgtc
     6121 atgtctcctg gcggacgata aatgattacg gcgggttgag tgcaaagaag gagcaaaatc
     6181 tgctctgaaa caggctcgga aaacgtttgc gctttttttg ccacaggtca attccctttt
     6241 ggtccgaact cgcacataat acgcccccgg tttgcacacc gggaatccag gagagttcat
     6301 gtactacccc ttcgttcgta aagccctttt ccagctcgat ccagagcgcg ctcatgagtt
     6361 tacttttcag caattacgcc gtattacagg aacgccgttt gaagcactgg tgcggcagaa
     6421 agtgcctgcg aaacctgtta actgcatggg cctgacgttt aaaaatccgc ttggtctggc
     6481 agccggtctt gataaagacg gggagtgcat tgacgcgtta ggcgcgatgg gatttggatc
     6541 gatcgagatc ggtaccgtca cgccacgtcc acagccaggt aatgacaagc cgcgtctctt
     6601 tcgtctggta gatgccgaag gtttgatcaa ccgtatgggc tttaataatc ttggcgttga
     6661 taacctcgta gagaacgtaa aaaaagccca ttatgatggc gtcctgggta ttaacatcgg
     6721 caaaaataaa gatacgccgg tggagcaggg taaagatgac tatctgattt gtatggaaaa
     6781 aatctatgct tatgcgggat atatcgccat caatatttca tcgccgaata caccgggatt
     6841 acgtactctg caatatggcg aagcgctgga tgatctctta accgcgatta aaaataagca
     6901 aaatgatttg caagcgatgc accataaata tgtgccgatc gcagtgaaga tcgcgccgga
     6961 tctttctgaa gaagaattga tccaggttgc cgatagttta gttcgccata atattgatgg
     7021 cgttattgca accaatacca cactcgatcg ttctctggtt cagggaatga aaaattgtga
     7081 tcaaaccggt ggcttaagtg gtcgtccgct tcagttaaaa agcaccgaaa ttattcgccg
     7141 tttatcacag gaattaaatg gtcgcttacc gatcatcggt gttggcggca tcgactcggt
     7201 tatcgctgcg cgtgaaaaga ttgctgcggg ggcctcactg gtgcaaattt attctggttt
     7261 tatttttaaa ggtccgccgc tgattaaaga aatcgttacc catatctaat tatttcttcg
     7321 aattcctctt tataagataa tcagggcttt attttaagcc ctggttgttt tatattcatc
     7381 gctgttgctt atttagacat tttgtacttt tattgttgag gttattaagc gaagcgacaa
     7441 tggattgtgt tgctgcggtt ttatagggta ggggagaggc aaatgcgaat taaaccagat
     7501 gataactggc gttggtatta cgatgaagag cacgatcgaa tgatgctcga tttagccaat
     7561 ggtatgctat ttcgctcacg ttttgcgcgc aaaatgttga ctccagatgc cttttctccc
     7621 gcaggctttt gcgttgacga cgccgcgctc tatttctcat ttgaagaaaa atgccgcgat
     7681 tttaatttat ctaaagaaca aaaagccgaa ctggtgctta atgcgctggt agcaattcgt
     7741 tatctcaaac cgcaaatgcc gaaaagctgg cattttgttt cccatggtga aatgtgggtg
     7801 cccatgccgg gcgatgcggc ctgcgtttgg ttgagcgaca ctcacgagca agtcaatttg
     7861 ctggtcgttg aatctggcga aaatgccgca ctatgcctgc tggcacaacc ctgcgttgtt
     7921 attgcgggtc gcgcgatgca gctgggcgac gccatcaaaa tcatgaacga caggctgaaa
     7981 ccgcaggtta atgttgacag cttcagcctc gaacaggcag tctaacgcgt cagcttaagc
     8041 gcagtcttcg gtacacagct acagcaaaga atggtgccat catcgcccat tgctgatttt
     8101 ttcagcggcg tgacttcgcc ttctaaaagc tgaacacggc aacttccaca aatgcccgcg
     8161 cggcaagaat aagggatacg aattccctga ttttctaatt gctccagcaa cacttgttgg
     8221 ttatttccac gaaatgcctg tccctgccag tcaatatcta catttgcgtc cggttgttgc
     8281 gtgatgttgg cggtatcatc agcggcagct gcgccgtaaa tttttgccgg agccgttgcc
     8341 agaatttcca cctcatcgcc aacgcgaatc acgccgctat tacgggcaat taaattctga
     8401 ccaaaatcga catcgccgtt atcctgggca gtgcggaaag attgcaatgt ttttaatggt
     8461 tcgcctgccg gatgtttttg ccctttttct gggctgacgg tggtgaaaat acagcggcta
     8521 caaggtttaa ccacatcaaa taccacatca ccaatgcgaa tcactttcca gcgatcttct
     8581 tcccaggctg acgcgccgga aaccaccaga ttagggcgga attgctccat ttttacactg
     8641 gccggacaac gttgttggag atcacgtaac gaggcttcgt tagcaagaag gtaaggatag
     8701 ccatcagcaa atgacagagg tacagtgttg tggcgtttca cgcgccgggt catttgtggc
     8761 cccacccagc gcaactgcac ttcgcgggag aaaaatccac ttagccattt gttgatcgcg
     8821 tctggtgcaa ttcgcgcggt aaaatgtgtg ccccaaactt cggttggtgc gtcttgtgtg
     8881 gcgaaatcag caaaacgaac atatgcgcta ctgccatctg gtgcggtgag atgcaagcca
     8941 tcatgcacag gtgaaggagt aaaccgtacc atctggggaa actggcgagc ggtaataaac
     9001 gtaccgtcag gttccgtgat cataaagatg cgatcgaagg ccaggccact gatatctgcc
     9061 agggcatgtg taagaccaat gccgcgcatc gatttaacag gatgaacaaa aagccgggtt
     9121 aatgtcgcca cgatgcggtc ctcaaatgaa aataagccct caactttatg acatgacgcg
     9181 cttattagct ataatgcgca acaattttct tagcgcctgt cccattaggc tattttattt
     9241 gtcagtttgg cccagggaag tgctcgaaat cctcacgtac ttgtgtacgc tccggtttct
     9301 ccgcgctgtc catgtccaga ctgactgcaa caattacgcc tactgcggta ggctcttaga
     9361 gtaaaagtga cgatatgaat tctctgtttg ccagtacggc ccgtgggctg gaagagctgt
     9421 taaaaactga actggaaaac ctgggggccg ttgaatgcca ggtggttcag ggtggggtcc
     9481 atttcaaggg cgacacacgg cttgtttacc agagcctgat gtggagccgc ctggcctcgc
     9541 gtattatgtt gccgctgggc gagtgtaagg tttacagcga tttagacctc tatctcggtg
     9601 ttcaggcgat caactggaca gagatgttta atcctggcgc gaccttcgct gtccacttca
     9661 gtggtttgaa tgacaccatc cgcaacagtc agtacggtgc gatgaaagtg aaagacgcga
     9721 ttgtcgatgc tttcacgcgg aaaaatctgc cgcgtccaaa tgttgatcgc gatgcgccgg
     9781 atattcgcgt taacgtctgg ctgcataaag aaaccgccag tatcgccctt gatctcagtg
     9841 gtgatggttt acatctgcgt ggctatcgcg atcgtgctgg tattgcgccg atcaaagaaa
     9901 ccctggcagc cgcgattgtt atgcgatccg gctggcagcc aggaacaccg ctgctcgatc
     9961 cgatgtgtgg ttccggtacg ttgttgattg aagcggcgat gctggcgacc gatcgcgcac
    10021 caggcttgca ccgtggacgt tggggcttta gcggctgggc acagcatgat gaagccatct
    10081 ggcaggaagt gaaagcagaa gcgcaaaccc gcgcccgtaa aggcctggct gagtatagct
    10141 ctcattttta cggttcggac agcgacgcac gggtgattca acgtgcgcgc actaacgccc
    10201 gtcttgcggg gattggtgaa ctgatcacct ttgaggtgaa agatgtcgcg caactgacca
    10261 atccgctgcc gaaagggccg tacggtacag tgttgagcaa cccgccttac ggcgaacgtc
    10321 tggacagcga accggcgctg attgcgctgc atagcctgct gggccgaatc atgaaaaacc
    10381 agttcggcgg ctggaatctc tctttattca gtgcctcgcc ggatctgtta agttgcctgc
    10441 agttgcgtgc agacaaacag tacaaagcga aaaacggtcc attagactgc gtgcagaaaa
    10501 attaccacgt tgccgaaagc acaccagaca gcaaaccggc aatggtggca gaagactacg
    10561 ccaaccgtct gcgtaagaac ctcaaaaaat tcgagaagtg ggcgcgtcag gaaggcattg
    10621 aatgctaccg cctgtacgac gccgatctac cggaatacaa cgttgccgtt gatcgttatg
    10681 ccgactgggt ggtggtgcag gagtatgcgc cgccaaaaac tattgatgcg cacaaagcgc
    10741 gtcagcgtct gttcgatatt atcgctgcaa ccatttcggt actggggatt gcgccaaaca
    10801 aactggtgct gaaaacccgt gaacgccaga agggcaaaaa tcagtaccag aaactgggcg
    10861 agaagggcga gtttctcgaa gttaccgaat ataacgctca cttgtgggtg aacctgacgg
    10921 attacctcga taccggtctg ttcctcgatc accgtatcgc ccgtcgtatg ctcggtcaga
    10981 tgagcaaagg caaagatttc ctcaacctgt tctcttatac cggcagcgcc accgtgcacg
    11041 cgggattagg cggtgcacgc agcactacca ccgtggatat gtcgcgtact tatctggagt
    11101 gggcagaacg caacctgcgt ctgaatggcc taaccgggcg tgcgcatcgc ctgattcagg
    11161 ccgattgcct ggcgtggctg cgtgaggcta atgaacagtt cgatctgatc tttatcgatc
    11221 caccaacctt ctctaactcg aaacgaatgg aagatgcgtt tgatgttcag cgcgatcatc
    11281 tggcgctgat gaaagatttg aaacgtctgc tgcgtgcagg tgggacgatc atgttctcga
    11341 acaacaaacg cggtttccgt atggatcttg acggactggc aaaactggga ctgaaagcac
    11401 aagaaattac gcaaaaaacg ctctcccagg atttcgcccg taaccgccag atccacaact
    11461 gctggctgat taccgcagcc tgaaaggaat agtaatgtca ttaatcagta tgcatggcgc
    11521 atggctgtcg ttcagcgacg cgccgcttct cgataacgca gaactgcata tcgaagataa
    11581 cgaacgtgtt tgtctggtgg gccgcaacgg cgcaggcaaa tcgacgttaa tgaaaatcct
    11641 caaccgtgaa caagggctgg atgacggtcg cattatttac gagcaagatt tgattgtagc
    11701 tcgtctgcaa caggatccgc cgcgtaacgt tgaggggagt gtttatgatt tcgttgccga
    11761 aggcattgaa gaacaagcgg aatacctgaa acgctatcac gatatttcgc gcctggtgat
    11821 gaacgacccg agcgagaaaa atctcaacga actggcgaag gttcaggaac agctggatca
    11881 ccacaacctg tggcagctgg aaaaccgcat taacgaagtg ctggcgcaac tggggttaga
    11941 tcctaacgtt gcgctgtcgt cgctttccgg cggctggttg cgtaaagcgg cattaggacg
    12001 cgcgctggtg agcaatccgc gcgtattgct gctcgacgaa ccgactaacc acctggatat
    12061 cgaaaccatc gactggctgg aagggtttct caaaaccttc aatgggacga ttattttcat
    12121 ctcccacgac cgttcgttta tccgcaatat ggcgacgcgt attgttgatc tcgatcgcgg
    12181 caagctggtg acctatccag gaaattacga ccagtacctg ctggaaaaag aagaagccct
    12241 gcgcgtggaa gaactacaaa atgccgagtt cgatcgcaaa ctggcgcagg aagaggtgtg
    12301 gatccgccag gggatcaaag cacgccgtac ccgtaatgaa ggccgcgtac gcgccctgaa
    12361 agcaatgcgt cgcgagcgtg gcgaacgtcg cgaagtgatg ggtaccgcaa agatgcaggt
    12421 ggaagaggcc agccgctccg gtaagatcgt tttcgaaatg gaagacgttt gctaccaggt
    12481 cgacggtaag caactggtga aagatttttc tgcccaggtt ctacgtggcg acaaaattgc
    12541 cctgattggt ccgaatggat gcggcaaaac cacgctgctg aaactgatgc tcggtcagct
    12601 tcaggcggac agcgggcgta ttcacgttgg caccaaactg gaagtggctt atttcgatca
    12661 gcaccgtgcg gaactggatc ccgataaaac ggtgatggat aaccttgctg aaggtaagca
    12721 agaggtgatg gttaacggca agccacgcca tgttttgggc tatttgcagg acttcctgtt
    12781 ccatccgaaa cgggcgatga cgccggtacg tgcgctttct ggcggtgagc gtaaccgctt
    12841 gttgttggcg cgtttgttcc tcaaaccaag caacttattg attcttgacg aaccgaccaa
    12901 cgatcttgat gttgaaacgc tggaactgct ggaagaactg atcgacagct accagggcac
    12961 ggtattgctg gtgagccacg atcgtcagtt tgtcgataac accgttacag aatgctggat
    13021 cttcgaaggc ggcggtaaaa ttggtcgtta tgtcggcggt tatcatgatg cccgtggtca
    13081 gcaagaacag tatgtggcgc tcaaacagcc tgcggtgaaa aaaatcgaag aagccgccgc
    13141 gccaaaagca gaaactgtaa aacgcagcag tagcaaacta agctataaat tgcagcgcga
    13201 actggagcag ctaccgcaat tgctcgaaga tctggaggcg aagctggaag ccctacagac
    13261 gcaggtggcg gatgcttcct tcttcagtca gccgcatgag cagacgcaaa aagtgctcgc
    13321 cgatatggct gctgcagagc aggagctgga gcaagccttt gaacgctggg agtatcttga
    13381 agcgttaaaa aatggtggct gatcgcaaaa tagtcgattt aaacagagac agctgacaac
    13441 ggtaaaattg cctgatgaac tgggtttatc aggcatgaaa accaacactt tgttagtcat
    13501 ctgtaaaata gcgcatcatt aaggagtacc aatgtgcgaa catcatcatg ccgcgaagca
    13561 catcctgtgc tcgcagtgtg acatgctggt ggcgttaccg cgccttgagc atggtcagaa
    13621 agcggcatgt ccccggtgtg gcacaacgtt aaccgtggcg tgggatgccc ctcggcagcg
    13681 tcccaccgcc tatgcgttgg ctgcactgtt catgctgttg ctgtccaact tgtttccttt
    13741 tgtgaatatg aacgttgcag gagttaccag tgaaattaca ttactggaaa ttcccggcgt
    13801 gcttttttct gaggactacg ccagcctcgg cacctttttc ctgttgtttg tgcaactggt
    13861 tcccgcgttt tgtctgataa ccattctgtt actggtgaat cgcgcggaat taccggtccg
    13921 tttaaaagag caactggcac gggtgctttt tcaactcaaa acctggggaa tggcggagat
    13981 tttcctcgcg ggtgtgctgg tcagtttcgt taaactgatg gcttacggca gcattggtgt
    14041 aggcagcagc tttctcccat ggtgtttatt ttgtgtcctg caactgcgcg cttttcagtg
    14101 cgttgatcgt cgctggttat gggatgacat cgccccgatg ccagaactgc gccagccgct
    14161 aaaaccaggc gtcacgggga tacgtcaggg gctgcgttca tgctcctgtt gtacggcaat
    14221 ccttcctgct gacgaacccg tatgcccgcg ctgtagtacc aaagggtacg ttcggcgtag
    14281 aaacagcctg caatggacac tcgcgctgct tgttacgtcc atcatgctgt atcttccggc
    14341 taatattttg cccatcatgg tgacggattt attaggctcg aagatgccgt cgacgattct
    14401 cgctggggtc atcctgttat ggagcgaagg atcttatccc gtcgctgcgg ttatctttct
    14461 ggccagtatt atggtgccaa cgttaaagat gatcgccata gcgtggttgt gttgggatgc
    14521 caaagggcat ggcaagcgcg acagtgaaag aatgcatttg atttatgaag ttgttgagtt
    14581 tgtaggccgc tggtcgatga ttgacgtttt cgttatcgcg gtgctctcgg cgctggtgcg
    14641 tatgggaggt ttaatgagta tttatccggc aatgggtgca ttaatgtttg ctttagtcgt
    14701 cataatgaca atgttttctg ctatgacgtt tgacccacgt ttgtcgtggg atcgtcaacc
    14761 tgaatcagag catgaggagt cctgacagta tggaatctaa taatggggaa gccaaaatcc
    14821 agaaagtgaa gaactggtct cccgtgtgga tatttcctat cgtcacggcg ctcattgggg
    14881 cctgggttct tttttatcat tacagccatc aggggccgga agtgaccctg atcaccgcga
    14941 atgcggaagg aatagaaggt ggtaaaacca ccattaaaag ccgtagcgtt gacgtcggcg
    15001 tggttgaaag cgccacactg gctgatgatt tgacgcacgt tgaaatcaaa gcgcggctga
    15061 attccggtat ggaaaaattg ctgcataaag ataccgtctt ttgggtggtt aaaccgcaga
    15121 ttggtcgaga agggattagc ggcctgggaa cgctgctgtc tggtgtttat atcgaactgc
    15181 agccaggcgc gaaaggcagc aaaatggata aatacgattt gctggactca ccaccgttgg
    15241 ccccgcctga tgcgaaaggt atccgtgtga ttctcgatag caaaaaagcc gggcagctct
    15301 cgccaggaga tcccgtgctg ttccgtggct atcgggtagg ttcggttgaa accagcacct
    15361 tcgatacgca aaaacgcaat atcagctatc aactgttcat caatgcacct tatgaccgac
    15421 tggtgaccag caatgttcgc ttctggaaag atagtggcat tgcggttgat ctgacgtcag
    15481 cggggatgcg tgtggagatg ggctcattga caacgctgct tagtggcggt gtcagctttg
    15541 atgtgccgga aggtctggat ttagggcagc cagtggcacc gaaaacagct tttgttttgt
    15601 atgatgatca gaagagcatt caggattcgt tgtacaccga tcacattgat tatctgatgt
    15661 tctttaaaga ttcggtacgc ggtctgcaac cgggagctcc ggtagaattc cggggtattc
    15721 gcctgggtac cgtaagcaaa gtgccattct ttgcgccgaa tatgcgtcag acatttaacg
    15781 atgattaccg tattccggta ctgattcgta tcgagccaga gcggctgaaa atgcagcttg
    15841 gcgaaaatgc ggatgttgtt gagcaccttg gcgaattgtt gaaacgtggt ttacgcggat
    15901 cgctgaaaac cggaaacctg gtcactggtg cactgtatgt tgatctcgat ttctatccaa
    15961 atacgcctgc aataaccggt attcgtgaat ttaatggtta tcagattatc ccgaccgtta
    16021 gcggcggtct ggcgcaaatc cagcaacgac taatggaagc gttggataag atcaacaaac
    16081 tgccattgaa tccgatgatt gaacaggcaa ccagtacgct ttctgaaagt cagcgcacaa
    16141 tgaaaaacct gcaaacgacg ctggatagca tgaacaagat cctcgccagc cagtcgatgc
    16201 agcagttacc gacggatatg cagtcaacgt tgcgtgaatt gaatcgcagt atgcagggct
    16261 tccagcctgg ctccgcagcc tacaacaaga tggtggcgga tatgcagcga cttgatcagg
    16321 tgttgcgaga actgcaaccg gtgctgaaaa cgctcaacga gaagagtaac gcgctggtat
    16381 ttgaagcgaa ggacaaaaaa gatccagagc cgaagagggc gaaacaatga aaaagtggct
    16441 agtgacgatt gcagcactgt ggctggccgg atgcagctcc ggcgaaatta ataaaaacta
    16501 ttaccagtta cctgtggtgc agagcggtac acaaagtacc gccagccagg gtaatcgcct
    16561 gttatgggta gagcaggtca ctgttcctga ctatctggcg ggaaatggtg tggtttatca
    16621 aaccagtgat gtgaagtatg tgattgccaa caacaacttg tgggccagcc cattggatca
    16681 acagttgcgc aacaccctgg ttgccaacct gagcacgcaa ctgcccggct gggtggttgc
    16741 ctcccagcct ctgggaagcg cccaggacac gctcaatgtt accgtaacgg agtttaacgg
    16801 tcgctatgat ggcaaggtca ttgtcagtgg ggagtggctg ttgaaccacc agggacaact
    16861 gatcaaacgt ccgttccgtc tggaaggggt gcaaactcag gatggttacg atgaaatggt
    16921 taaagtgctg gccagtgtct ggagtcagga agccgcttct attgcgcaag agataaagcg
    16981 tctaccttaa ttataaagat ttgtaaatat aaccgcctcc ggtatgttgc ctgaggcggt
    17041 ttttttgttt ctaaggtgcg gaaaaagttg ttcctcttca cattttttgt acaactgaca
    17101 tgcccgtgta gctcacaaat atgacagtgg cgtgaatttt gcgcattgac ggcagttatg
    17161 attcgcggta ttgcttaact gtgattgcac atttagtaat cactgttttc ttttccacca
    17221 gaaaccagta tgagggaaac gaggcatgaa gagacaaaaa cgagatcgcc tggaacgggc
    17281 acatcaacgt ggttatcagg ccggcatcgc cggacgctca aaagaaatgt gtccctatca
    17341 gacgctgaat caaaggtcac aatggctggg aggctggcga gaagccatgg cggacagggt
    17401 agtaatggcc tgattctgtc tctttaaaaa gaaacctccg cattgcggag gtttcgcctt
    17461 ttgatactct gtctgattat aatcagaagg cagacgtatc ctggaacaga ccgactttca
    17521 ggtcgctggc ggtatagatc aaacgaccat caaccagcac ttcgccatcc gccaggccca
    17581 taatcagacg acggttaaca atgcgtttaa agtgaatacg gtaggtcact tttttcgctg
    17641 tcggcagtac ctgaccagtg aatttcactt cgccaacgcc cagcgcgcgg cctttacctt
    17701 cgccgcccag ccagccgagg tagaacccta ccagctgcca cattgcgtcc aggcccaggc
    17761 atcccggcat aaccggatcg ccaataaagt ggcatccgaa gaaccacaga tccggattga
    17821 tatccagttc tgcttcaaca taccctttgt cgaagttacc acccgtttcg gtcattttga
    17881 ccacacggtc catcatcagc atgttcggtg ctggcaattg cgggccttta gcgccaaaca
    17941 gttcaccgcg accagaggca agaaggtctt cttttgtata ggattcgcgt ttatctacca
    18001 tgttctctgt aagccttatt ttattgaagc acgcaggata gctaacacgt gtacgctgaa
    18061 caagtccgat cagttcggaa taaaccagtt cagccaacgt aatggccatg gaaaacggtg
    18121 acgtccttcc tgttgcgatg cttgcgcgat acgttcctgg atggtttgca tcagcgtcgt
    18181 ttggccttcg ccatcccaca ccagatttaa taataacggc agtgcgtcag tcacatcgtc
    18241 tactgcccag atggtgaatt tgccttcctc taccgctttc aacagttcac tgggaagact
    18301 taaatggcga acgttagcag tggggataat gacaccttgt ttcccggtta actcacgttg
    18361 ctggcaaata gcaaagaagc cttcgatttt ctcatttaaa ccaccgaccg gctgggcacg
    18421 accgaactga tcgactgaac ctgtgatagc gatactctga ttcaccggca catcggcgag
    18481 ggcgcttatc agggcgcaga gttcagccat cgaggcacta tcgccatcca cttcactgta
    18541 tgactgctca aatgtcagcg atgctgagaa ggggatctgt tgctcaagct gtagttccga
    18601 catcaggaac gcctgcatga tcatcatccc tttcgcatgg atattgccgc caagctccgc
    18661 tttgcgttcg atgtcggtga actcaccatc gccaatatgc acaacgcagc taatgcgaga
    18721 aggttcgcca aaagcgcgtg gatgacccgg aaattcaatg accgaaaggg cgttaatttg
    18781 cccgatgcgt tcgccttcgg tttcaatcag gatttgctcc tgaaggatct catcctgcat
    18841 gcgttcagcg aggaaacctt cgcgccattc acgctgctgt agcattaagt ttagctgctc
    18901 gccggagaag gtgtcgccat cgcacaggga ggcgacctct ttacactggc ggaggatcca
    18961 ctgcgggcta agcggaagtg tttcttgttc accggtgtag cgtgctgctt cgcggataag
    19021 caccggccag gcatccgctc ccggtgcagg taagtgatta tgtctggcgg tcaatgttac
    19081 ccagcgacac cactggctta ctgactccgc atcgacaatc tgcagagtat cttcaaattc
    19141 gctataaata gcctgctctg aaagctctgg ttccatctcc tggaaatcag ccaatgattc
    19201 gcgttcgcct accagaatga ctttcagctt caatggcatc gaaggcacag agacggggag
    19261 agggcgcgac tcatcaaacg caacccagtc aaaacgctcg cggttaacga tatttttcag
    19321 ccgcatccac agcagaggtt gcgccagcag tgtacgcaaa gagatgatga gaataccgcc
    19381 atttgcctga tgcaccagac ctggctgcag ggtaatgtcg ccattaaact ggcgcaggca
    19441 gccaaagagt tgttccgctt ctacccagtc ggcagcgaca acttgtgtta aagtcgcaaa
    19501 attatcatct gcactcactg cgttacgtaa gcggatggtg tgcccggaaa cctcatagtg
    19561 accgcccacc agttggcctg catcgctttg tagcgcacgc gcggcatcgg caataagatt
    19621 cagatactca gactcttccg gggccttcgc cagcataaaa gaggaggatg ctcgcgtatg
    19681 cagcaactgc tccagcgcaa attgcagtcg cggttgagta tcactgaata aaggatcgtt
    19741 ttcgtcaatc aaatgtggct gagcaaatat ttcctgatag ctatcggtat caggaaccag
    19801 gtcacgccat gcaagtttcg taatggtcaa agttgatgtt ttttagtctg ttgtcaaagc
    19861 cgcgattata ccgtaaccgg cactgcagca cacgtagaaa gcaccgacaa tactcctggc
    19921 atgggcgtta aagctcacag gatggagatt cttttcttca ctggcccaaa aagctgatat
    19981 tctgtaaaga gttacacggt aacattgaga tcgctatgaa atatcaacaa cttgaaaatc
    20041 ttgaaagcgg ttggaaatgg aagtatctgg tgaagaagca tcgcgaaggg gagttaatca
    20101 cccgttacat agaagccagt gccgcccagg aagccgtcga tgtgttgctc tcgctggaaa
    20161 atgaacccgt gctggtaaat ggctggattg acaagcatat gaatccggaa ctggtcaatc
    20221 gcatgaagca aaccattcgg gccagacgga agcgccattt taatgcggaa caccagcata
    20281 cgcgcaaaaa atcgatcgat ctggaattta ttgtctggca acgtctggct ggtcttgcgc
    20341 agcgtcgcgg taaaacgctt tctgaaacga ttgttcagct gattgaagat gcggaaaaca
    20401 aagagaaata cgcgaataaa atgtcttctt tgaagcagga tctgcaggca ttgctgggta
    20461 aggaataact gacgaaagtc agttcaattt tctatagacg aaaaaaaacc ccgcagcagc
    20521 ggggttttta ccagacgaga acttaagcct gcggctgagt tacaacgtct ttgatgcctt
    20581 taacttcgat ctctacgcga cgatccggag ccaggcagtc gatcagtgca gcacgctgtt
    20641 tcacgttgtc acaggtgttg ccagtaaccg ggttggattc gcccatacca cgtgcggaga
    20701 ttttgtctgc cgggatacct ttagagatca ggtaatcaac aacagactga gcacggcgct
    20761 cggacagacc ctggttgtaa gcgtcagaac cgatgcggtc ggtgtaaccc agaacaacta
    20821 cggaaccgtc tttcggatcc aggttgctca gctggctgta cagctgatcc agagcagcct
    20881 gaccttccgg tttcagggtt gctttgttga aggtgaacag aacgtcagac ttcagagtga
    20941 agtgcttggt ctgtacttcc ggtgccggag ctggagccgg agcaactact ggagctgctt
    21001 cgccctgacc gaaacggtag gaaacaccca ggctcagcat gccgttgtcc ggacgagtac
    21061 cgatggtgtg tgcgtcaccg atgttgttgg tccactggta ttccagacgg gtagcgattt
    21121 caggagtgat cgcatactca acaccgccag cgaagaccgg agaaacgccg gtatcgtggt
    21181 ctttaaaaga tgcgccacca ggtacgttag ccttggtgtc tgcacgccat accataccac
    21241 ccagacgagt gtagatgtcc agatcgtcag tgattgggta acccagttta gcggtcagct
    21301 gaacgccctg agctttgtat gcgccgttga tgttgtcgcc tttgtacggc atacgaccta
    21361 accagtcgta acccatttca aagccaacat acgggttaac ctggtaacca ccaaaagcac
    21421 ctgcacccag ttggttttcg tgggtcggac cattgttagg aataaaacca gtgtcatggt
    21481 actgggacca gcccagttta gcaccagtgt accaggtgtt atctttcgga gcggcctgcg
    21541 ctacggtagc gaaaccagcc agtgccactg caatcgcgat agctgtcttt ttcatttttt
    21601 gcgcctcgtt atcatccaaa atacgccatg aatatctcca acgagataac acggttaaat
    21661 ccttcaccgg gggatctgct caatattaac tctaccgata tcttcggctt atgccgagca
    21721 cccctggcga tgtaaagtct acaacgtagt tgaaaactta caagtgtgaa ctccgtcagg
    21781 catatgaaaa aaaagtcttg tataaggtac gtttaatctt ttttgtcagc gacaatttac
    21841 agaagaaaat cgcggaaacc gcttcagaca agcc
//
LOCUS       DACBVI010000068        21698 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.068, whole genome shotgun sequence.
ACCESSION   DACBVI010000068 DACBVI010000000
VERSION     DACBVI010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.068"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..2961
                     /locus_tag="IU325_12590"
     CDS             <1..2961
                     /locus_tag="IU325_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809858.1"
                     /translation="WNATAGAFSAAHGTEATSKITNVKDGDLTAGSTDAVNGSQLKTT
                     NDAVAANTTNIATNTTNITNLTDAVDSLGDDSLLWNATAGAFSAKHGTNGTDSKITNL
                     LAGTVSSDSTDAINGSQLYGLADSFTSYLGGGADISDAGVLTGPTYTIGGTDYNNVGD
                     ALAAINTSFSTSLGDALLWDATAKGGDGAFSAGRGKDNTASIITNVADGAISSTSSDA
                     INGSQLYDTSKYIADTLGGDAEVNADGTITAPTYAIAGGSYSNVGDALEAIDTTLDDA
                     LLWDATANGGNGAFSAGRGVDKTASVITNVADGAISSTSSDAINGSQLYTTNKYIADA
                     LGGDAEVNADGSITAPSYTIANAEYNNVGDALDALDDNALLWDATANDGAGAYNASHD
                     GKASIITNVADGNIGEGSTDAINGSQLFNTNMLIQQNSEIINQLAGNTSETYIEDNGA
                     GINYVRTNDNGLAFNDASASGIGATAVGYNAVASGESSVAIGQGSSSTVDTGIALGSS
                     SVSSRVIVKGSRDTSVSEEGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHD
                     AVTVRQLQNAIGAVATTPTKYFHANSTEEDSLAVGEDSLAMGAKTIVNSNAGIGIGYG
                     AYVDANALNGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSV
                     GSEDGQRQITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLNNQVTNLDTRVTNI
                     ENGIGDIVTTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVANEE
                     NTISVGSSTNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLG
                     GGNGGTTRISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSG
                     GIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNS
                     QGEYSAALGAGIQW"
     gene            3259..4914
                     /gene="lldP"
                     /locus_tag="IU325_12595"
     CDS             3259..4914
                     /gene="lldP"
                     /locus_tag="IU325_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HAP5809859.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVIYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYTAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            4914..5690
                     /gene="lldR"
                     /locus_tag="IU325_12600"
     CDS             4914..5690
                     /gene="lldR"
                     /locus_tag="IU325_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HAP5809860.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGEKEKIQLCFEATLSEDPDLASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGDHNEHSREKNA"
     gene            5687..6877
                     /gene="lldD"
                     /locus_tag="IU325_12605"
     CDS             5687..6877
                     /gene="lldD"
                     /locus_tag="IU325_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HAP5809861.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQELGKELPAALAPIAK
                     GNAA"
     gene            6925..7398
                     /gene="trmL"
                     /locus_tag="IU325_12610"
     CDS             6925..7398
                     /gene="trmL"
                     /locus_tag="IU325_12610"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP5809862.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(7451..8272)
                     /gene="cysE"
                     /locus_tag="IU325_12615"
     CDS             complement(7451..8272)
                     /gene="cysE"
                     /locus_tag="IU325_12615"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP5809863.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(8351..9370)
                     /gene="gpsA"
                     /locus_tag="IU325_12620"
     CDS             complement(8351..9370)
                     /gene="gpsA"
                     /locus_tag="IU325_12620"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP5809864.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(9370..9837)
                     /gene="secB"
                     /locus_tag="IU325_12625"
     CDS             complement(9370..9837)
                     /gene="secB"
                     /locus_tag="IU325_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HAP5809865.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(9900..10151)
                     /gene="grxC"
                     /locus_tag="IU325_12630"
     CDS             complement(9900..10151)
                     /gene="grxC"
                     /locus_tag="IU325_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HAP5809866.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(10293..10724)
                     /locus_tag="IU325_12635"
     CDS             complement(10293..10724)
                     /locus_tag="IU325_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP5809867.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVIVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            10969..12513
                     /gene="gpmM"
                     /locus_tag="IU325_12640"
                     /gene_synonym="pgmI"
     CDS             10969..12513
                     /gene="gpmM"
                     /locus_tag="IU325_12640"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HAP5809868.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            12523..13806
                     /gene="envC"
                     /locus_tag="IU325_12645"
     CDS             12523..13806
                     /gene="envC"
                     /locus_tag="IU325_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HAP5809869.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRSSLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            13810..14769
                     /locus_tag="IU325_12650"
     CDS             13810..14769
                     /locus_tag="IU325_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HAP5809870.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDMSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASAN"
     gene            complement(14756..15790)
                     /gene="waaH"
                     /locus_tag="IU325_12655"
     CDS             complement(14756..15790)
                     /gene="waaH"
                     /locus_tag="IU325_12655"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HAP5809871.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEAATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(16029..17054)
                     /locus_tag="IU325_12660"
     CDS             complement(16029..17054)
                     /locus_tag="IU325_12660"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP5809872.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(17064..18260)
                     /gene="kbl"
                     /locus_tag="IU325_12665"
     CDS             complement(17064..18260)
                     /gene="kbl"
                     /locus_tag="IU325_12665"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP5809873.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            18474..19406
                     /gene="rfaD"
                     /locus_tag="IU325_12670"
     CDS             18474..19406
                     /gene="rfaD"
                     /locus_tag="IU325_12670"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HAP5809874.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            19416..20462
                     /gene="rfaF"
                     /locus_tag="IU325_12675"
     CDS             19416..20462
                     /gene="rfaF"
                     /locus_tag="IU325_12675"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HAP5809875.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            20466..21425
                     /gene="rfaC"
                     /locus_tag="IU325_12680"
     CDS             20466..21425
                     /gene="rfaC"
                     /locus_tag="IU325_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HAP5809876.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTVREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVVCRAPLMNLNNLEAAAVYKKITLI"
BASE COUNT         5311 a         5731 c         5658 g         4998 t
ORIGIN      
        1 tggaacgcta cggcgggggc attcagcgcc gcgcacggta ccgaagccac cagcaaaatc
       61 accaacgtca aagacggtga cctgacggct ggcagcactg acgcggtaaa cggctctcag
      121 ctgaaaacca ctaacgatgc cgtggcagcc aacaccacca atatcgccac gaataccacc
      181 aacatcacca acctgactga cgctgttgac agtctcggtg atgactctct gctgtggaac
      241 gctacggcgg gtgcattcag tgccaaacac ggtaccaacg gtactgatag caaaatcacc
      301 aacttactgg caggcacggt atcctctgat agcactgacg ccattaatgg ctcacaactt
      361 tatggcttag ctgattcatt tacgtcatac cttggcggtg gtgctgatat cagtgatgcg
      421 ggtgtattaa ccgggccaac ctacactatt ggtggtactg actacaataa cgtcggtgat
      481 gctctggctg ccattaacac gtcatttagc acatcactcg gtgacgccct actctgggat
      541 gcgaccgcca agggcggtga cggtgcgttt agcgccggtc gcggaaaaga taacacggcc
      601 agcatcatta ctaatgttgc agacggtgca atttcctcta ccagcagcga cgctattaac
      661 ggctcacagc tctatgacac cagtaagtac attgccgata ctctgggtgg tgacgcagaa
      721 gtcaatgctg acggcacaat caccgcaccg acttatgcca tcgctggcgg cagttacagc
      781 aacgtcggtg acgcgctgga agcgatcgat accacgctgg atgacgctct gttgtgggat
      841 gcaacagcca atggcggtaa tggtgcattt agcgccggtc gcggagtaga taaaacggcc
      901 agcgtcatta ctaacgtcgc agacggtgca atttcctcca ccagcagcga tgctattaac
      961 ggctcacaac tgtataccac caacaagtac atcgctgatg cgctgggtgg tgacgcagaa
     1021 gtcaacgctg atggttctat tactgcgccg agttacacca ttgcaaatgc cgagtacaac
     1081 aacgtcggtg acgccctgga tgcgctcgac gataacgctc tgctgtggga tgcaacggcc
     1141 aatgacggcg caggtgccta caacgccagc catgatggca aggccagcat catcacaaac
     1201 gttgctgatg gtaacattgg cgaaggcagc actgacgcca tcaacggttc tcagctgttt
     1261 aacaccaata tgctgatcca gcagaacagc gaaatcatca atcagcttgc cggtaacacc
     1321 agcgaaacct acatcgaaga taacggtgcg ggcattaact atgtacgtac caacgacaac
     1381 ggcttagcgt tcaacgatgc cagcgcttca ggtattggcg ctacggctgt gggttataac
     1441 gctgtcgcct caggcgaaag cagcgtagcc attggtcaag gtagcagcag caccgttgat
     1501 accggtattg ccctgggtag cagttccgtt tccagccgtg taatagttaa aggttctcgt
     1561 gacaccagcg tgtcggaaga aggtgttgtg attggttatg acaccacgga tggcgaacta
     1621 cttggtgcgt tgtctattgg tgatgacggt aaatatcgtc aaatcatcaa cgtagccgat
     1681 ggttccgaag cccatgacgc cgttacggtt cgccagttgc aaaatgccat tggtgcagtc
     1741 gctaccacgc cgaccaaata cttccacgcc aactcaacgg aagaagattc actggcagta
     1801 ggtgaagact cgctagcaat gggcgcgaaa actatcgtta atagtaatgc gggtattggt
     1861 atcggttatg gtgcctacgt ggacgcgaat gcacttaatg gcattgctat cggtagcaac
     1921 gcgcgtgcaa accatgcaaa cagcattgct atgggtaatg gctcacagac gactcgtggt
     1981 gctcaaactg gctacaccgc ctacaacatg gacgcaccac agaactctgt gggtgagttc
     2041 tctgtcggca gtgaagacgg tcaacgtcag atcaccaacg tcgcggctgg ttcggctgat
     2101 accgatgcgg ttaacgtggg tcagttgaaa gtaacggacg cgcaggtttc ccagaatacc
     2161 cagagcatta ctaacctgaa caatcaggtg acgaatctgg atactcgcgt taccaatatc
     2221 gaaaacggca ttggcgatat cgtaaccacc ggtagcacca aatacttcaa gaccaacacc
     2281 gatggcgtag atgccaacgc gcagggtaaa gacagtgttg caattggttc tggttccatt
     2341 gctgccgctg acaacagcgt cgcgctgggt accggttccg tggccaacga agaaaacacc
     2401 atctctgtgg gttcttctac caaccagcgt cgtatcacca acgttgctgc aggtgttaat
     2461 gccaccgatg cggttaacgt ttcgcagctg aagtcttctg aggcaggcgg cgttcgttac
     2521 gacaccaaag ctgatggttc tatagactac agcaacatca ctctcggtgg tggtaatggc
     2581 ggtacgactc gcatcagcaa cgtctccgct ggcgtcaaca acaacgacgc agtgaactat
     2641 gcgcagttga agcaaagcgt gcaggaaacg aagcaataca ccgatcagcg gatggttgag
     2701 atggataaca aactgtccaa aaccgaaagc aagttgagcg gtggtatcgc ttctgcaatg
     2761 gcaatgaccg gtctgccgca ggcttacact ccaggtgcca gcatggcctc tattggtggc
     2821 ggtacttaca atggtgaatc ggctgttgct ttaggtgtgt cgatggtgag cgccaatggt
     2881 cgttgggtct acaaattaca aggtagtacc aatagccagg gtgaatactc cgccgcgctc
     2941 ggtgccggta ttcagtggta atcatccatt aacttagtgt gactaagggc aattctctga
     3001 tgaggattgc ccttttcttt accagacatc tccctccacc aagaattggc cctaccaatt
     3061 attcgcttat ctgacctctg gttcacaatt ccccaattaa aactcacatc aatgttgcca
     3121 atgcataaca tttagttaac cattcattgt cattatccct acacaacaca attggcagtg
     3181 ccacttttac acaacgtgtg acaaggagat gagcatcaga ctcattacac gatgtgcgtg
     3241 gactccagga gacctgcaat gaatctctgg caacaaaact acgatcccgc cgggaatatc
     3301 tggctttcca gcctgatagc atcgcttccc atcctgtttt tcttctttgc gctgattaag
     3361 ctcaaactca aaggttatgt cgccgcctca tggacggtgg caatcgccct tgccgtggct
     3421 ttgctgttct ataaaatgcc ggtcgctaac gcgctggcct cggtgattta tggcttcttc
     3481 tacggtttgt ggcccatcgc gtggatcatt attgcggcag tgttcgtcta taagatctcg
     3541 gtgaaaaccg ggcagtttga catcatccgc tcgtctattc tttcgataac ccctgaccag
     3601 cgcctgcaaa tgctgatcgt cggtttctgt ttcggcgctt tccttgaggg agccgcaggc
     3661 tttggcgctc cggtagcaat taccgccgca ttactggtcg gtctgggctt taaaccactg
     3721 tacgccgccg gactgtgcct gattgttaac accgcgccag tggcatttgg cgcgatgggt
     3781 attccgattc tggtcgccgg gcaggtaaca ggcatcgaca gctttgagat tggtcagatg
     3841 gtggggcgtc agctgccgtt tatgaccatt atcgtgctgt tctggatcat ggcgattatg
     3901 gacggttggc gcggtatcaa agagacgtgg cctgcggtcg tggttgcagg tggttcgttt
     3961 gccattgctc aatacctcag ctctaacttc attgggccgg aactgccaga cattatctct
     4021 tcgctggtat cactgctctg cctgacgctg ttcctcaaac gctggcaacc agtgcgcgta
     4081 ttccgcttcg gtgacttggg ggcgtcacag gttgatatga cgctggctca caccggttac
     4141 accgcaggtc aggtgcttcg cgcctggaca ccgttcctgt tcctgaccgc caccgtaacg
     4201 ctgtggagta ttccaccgtt taaagccctg ttcgcttcgg gtggtgcgct gtatgaatgg
     4261 gtgatcaata ttccggtgcc gtacctcgat aaactggttg cccgtatgcc gccagtggtc
     4321 agcgaagcga cagcgtatac cgccgtgttt aagttcgact ggttctctgc caccggtacc
     4381 gccattcttt ttgctgccct gctctcgatt gtctggctga agatgaaacc atctgacgct
     4441 atcagcacct tcggcagcac gctgaaagaa ctggctctgc ctatctactc catcggtatg
     4501 gtgctggcgt tcgcctttat ctcgaactat tccggactat catcaacgct ggcgctggca
     4561 ctggcgcaca ccggccatgc attcaccttc ttctcgccgt tcctcggctg gcttggtgtc
     4621 ttcctgaccg gatcggatac ctcatctaac gccctgttcg ccgcgctgca agccactgca
     4681 gcacaacaaa ttggcgtttc tgacctgttg ttggttgccg ccaacaccac cggtggtgtc
     4741 accggtaaga tgatctctcc gcaatctatc gctatcgcct gtgcggcggt aggcctggta
     4801 ggcaaagagt cagatctgtt ccgctttact gtcaaacaca gcctgatctt cacctgtatg
     4861 gtgggcgtga tcaccacgct tcaggcttat gtcttaacgt ggatgattcc ttaatgattg
     4921 ttttacccag acgcctgtca gacgaggttg ccgatcgtgt gcgggcgctg attgatgaaa
     4981 aaaacctgga ggcgggcatg aagttgcccg ctgaacgtca actggcgatg caactcggcg
     5041 tatcgcgtaa ttcactgcgc gaggcgctgg caaaactggt gagcgaaggc gtgctgctta
     5101 gtcgacgcgg cggcgggacg tttattcgct ggcgtcatga cacatggtcg gagcaaaaca
     5161 tcgtccagcc gctgaaaacg ctgatggccg atgatccgga ttacagtttc gatattctgg
     5221 aagcccgcta cgccattgaa gccagcaccg catggcatgc agcaatgcgc gccacaccag
     5281 gcgaaaaaga gaaaattcag ctttgctttg aagcaacgct aagtgaagac ccggatctcg
     5341 cctcacaagc ggacgttcgt tttcatctgg cgattgccga agcctcacat aacatcgtgc
     5401 tgctgcaaac catgcgcggt ttcttcgatg tcctgcaatc ctctgtgaag catagccgcc
     5461 agcggatgta tctggtaccc ccggtttttt cacagctgac cgaacaacat caggcagtca
     5521 ttgacgccat ttttgccggt gatgctgacg gggcgcgtaa agcaatgatg gcgcacctta
     5581 gctttgttca caccaccatg aaacgattcg atgaagatca agctcgccat gcacgcatta
     5641 cccgcctgcc tggtgaccat aatgagcatt cgagggagaa aaacgcatga ttatttccgc
     5701 agccagcgat tatcgcgccg cagcacaacg cattctgccg ccgttcctgt tccactatat
     5761 ggatgggggt gcatattctg aatacacgct gcgccgcaac gtggaagatt tgtcagaagt
     5821 ggcgctgcgc cagcgtattc tgaaaaacat gtctgactta agcctggaaa cgacgctgtt
     5881 taatgagaaa ttgtcgatgc cggtggcgct ggctccggtg ggtttgtgtg gcatgtatgc
     5941 gcgtcgtggc gaagttcagg ctgccaaagc ggcggatgcg catggcattc cgtttactct
     6001 ctcgacggtt tccgtttgcc cgattgaaga agtcgctcca gccattaaac gtccgatgtg
     6061 gttccagctt tatgtgctgc gcgatcgcgg ctttatgcgt aacgcgctgg agcgagcaaa
     6121 agcggcaggc tgctcgacgc tggttttcac cgtggatatg ccgacaccgg gcgcgcgtta
     6181 tcgtgatgcg cattctggta tgagcggccc aaatgcggca atgcgccgtt acttgcaggc
     6241 ggtgacgcat ccgcaatggg cgtgggatgt gggcctgaac ggtcgtccac atgatttagg
     6301 taatatctcg gcttacctcg gcaaaccgac cggactggaa gattacatcg gctggctggg
     6361 gaataacttc gatccgtcca tctcatggaa agaccttgag tggatccgcg atttctggga
     6421 tggtccgatg gtgatcaaag ggatcctcga tccggaagat gcgcgcgatg cagtacgttt
     6481 tggtgctgat gggattgtgg tttctaacca cggtggccgc cagctggacg gtgtactctc
     6541 ttccgctcgt gcgttgcccg ctattgcaga tgcggtgaaa ggtgatatag ccattctggc
     6601 ggatagcgga attcgtaacg ggcttgatgt cgtgcgtatg attgcgctcg gtgccgacac
     6661 cgtactgctg ggtcgtgctt tcctgtatgc gctggcaaca gcgggccagg cgggtgtagc
     6721 taacctgcta aatctgatcg aaaaagagat gaaagtggcg atgacgctga ctggcgcgaa
     6781 atcgatcagc gaaattacgc aagattcgct ggtgcaagag ctgggtaaag agttgcctgc
     6841 ggcactagct ccgatagcga aagggaatgc agcttaaggg ttagacgaat atctgctatc
     6901 ctgcccccgc actaaggggg cagtatgcta aacatcgtac tttacgaacc agaaattccg
     6961 ccaaatactg gcaacatcat ccgtctttgc gctaataccg gctttcgtct gcatatcatc
     7021 gaaccaatgg gatttgcctg ggacgataag cgcctgcgcc gcgcggggct ggactatcac
     7081 gagtttaccg ccgttacgcg tcatcatgac tatcgcgcgt tcctcgaagc agaaaatccc
     7141 cagcgcctgt ttgccctcac cacgaaaggt acgcctgctc acagcgccgt aagctatcag
     7201 gatggcgatt atctgatgtt cggcccggaa acacgcggcc tgccagcaag cattcttgat
     7261 gccctgcccg ctgaacaaaa aattcgcatt ccaatggtgc cggacagccg cagcatgaac
     7321 ctgtccaatg cggtgtcggt cgtggtgtat gaagcctggc ggcagctggg gtatccggga
     7381 gcggtattga gagattagtt actgtatgcc ggatagatga ttacatcgca tccggcacaa
     7441 tcacaggacg tcagatccca tccccatatt caaacgtatg gttaataccg ttgaaatgct
     7501 gatccatgtc cattgacggt ttatcgctgt ctggtttacc gacaatgcgt gccggaacgc
     7561 cagcggcggt ggtatgcggc ggcaccggtt gcagcaccac ggaacctgcg ccaatcttcg
     7621 cgccgcgccc aacttcaata ttgccgagaa ttttcgcgcc cgcgccaatc atcacacctt
     7681 cacgaatttt cggatgacgg tcgccaccag atttacccgt accgccaagc gtcacagatt
     7741 gcagaatcga tacgtcgttt tcaatcaccg ccgtttcacc cacaacgatg cccgtggcgt
     7801 ggtcaagcat gataccgcgg ccaatttttg ctgccgggtg aatatcaacc tggaacgtta
     7861 cagaaacctg gttttgcaga aatattgcca gtgcgcggcg gccctgattc cacagccagt
     7921 gaccgatacg ataggcctgc aaggcatgaa aacctttcag atataacaat ggagtcgagt
     7981 atttatccac cgccgggtcg cgggtacgta ctgcctgaat atcacaggcc gcagaagcga
     8041 tcatttccgg gtcggcggcg taggcctctt ccaccacttc gcggatagca atggcaggca
     8101 taattggcga tgacagcttg ttcgccagca tgtagctcag tgcactgccc aggttttcgt
     8161 gcttgagtag cgtcgcgtgg taaaaactgg ccagcattgg ctcacaatcc gccagcgttc
     8221 tggcttcggc tttaatattg ttccagacaa tttccagttc ttcacacgac attgcttact
     8281 ccacacgatg agataatgac cggcccgttc tgcgcgggcc gggtcatagc ggtaacaaag
     8341 ttccctggga ttagtggctg ctgcgctcgt ccttgcgtgc acgacctaat aatgtcaatg
     8401 ctgcctcgcg cgcgtttttt ccgcaatata atacttgata aatttcctcg gttattggca
     8461 tttcaacgcc gaagcgatgc gccagttcgc ggacttcttt cgtattgcgg tagccttcca
     8521 ccacctgacc aatcttctcc tgcgcgcttt gtacatccat gccctgaccg agcatcatgc
     8581 caaaacggcg gttacgcgac tggttgtcgg tacaggtaag caccagatcg cccagccccg
     8641 ccatgcccat aaaggtggca ggatcggccc ccagcgccgc gccaagacgc gacatttcag
     8701 ccagcccacg ggtgatcagc gccgtacgcg cattcgcacc aaaaccgata ccgtcggaca
     8761 tccccgcgcc aatggcaatg acgtttttca ccgcgccgcc aagctgcacg ccaatgaaat
     8821 ccgggttgct gtaaacgcgg aaacttttgc cgcagtgcag cagctgctgg agatcatcgg
     8881 caaaggtctg gtcggtagag gccagcgaaa tggccgtcgg taaacctgcc gccagttctt
     8941 tcgcaaacgt tgggccagag ataaccgcca gcggaatttg atcgcctaac gcctcacgcg
     9001 ccacgtcctg caacagacgc ccggtttccg cttccagccc tttggtcgcc cacaccagac
     9061 gcgcatcagg acgcatcagc ggtttaatct gacgcagcac ttcaccaaag acatggctgg
     9121 gtacgacgac gagaatatta cggctggctg ccagcgcagt ggcgagatcg ctttcaagat
     9181 ggagcgtatc gggaaaaggc acatcgggga gaaacgcggc gttacagcgg tcgcgttcaa
     9241 gcgttgcgat atgttcaggg tcatggcccc agaggacaac ctcgtggcca tttcttgcca
     9301 gggtgatggc aagagcggtg ccgtacgagc cggcaccgat cacagtcatt gaagcattac
     9361 gttggttcat caggcatcct gatgttcttc agtaccttcg ccagcctgct gctgcaaata
     9421 gttcatgaac agcgcatcga agttaaccgg cgcaaggttc agttgcggga atgtaccgcg
     9481 ggataccatg ctggtgatgc actcacgagc atacgggaac agaatgttcg ggcagtatgc
     9541 acccaggcaa tgtgccatct gggtgccttc gatacccgcg atggagaaaa taccgccctg
     9601 ctgaacttca cacaggaacg cggtttcttc gcccagggag gccgttacgg taacacgcag
     9661 taccacttcg taaacgtcat ctgccagttg agaagatgcc gtatccagat caagtttaac
     9721 ttctggctgc caatctttct ggaaaacgtg cggcgcgttc ggcgcttcga aagaaatatc
     9781 cttggtataa atacgttgga tctggaaagt catttcggtg ttgttttgtt ctgacatgtg
     9841 tagaaaaccc ttaagtgttg tccttaaata ctgcgcaatg taatgccaac gttcacacgt
     9901 tatttcagca ggggatccag tccaccacgt gcatccaatg catacaagtc atcacagccg
     9961 ccaatgtgct gtgcgtcaat aaaaatctgg ggaaccgtgg tgcgaccgct gcgtttgatc
    10021 atctcttcac gcttagcggc gttgccatcg atcggcaact cctgaaaact cacgcccttg
    10081 ctgctcagca gtgctttcgc acgatggcaa tacgggcagg tttctttggt atagatttca
    10141 acattggcca taacttagct cctgtttttt taccctgaaa tttcatgttg cagggcggat
    10201 gcaaaatgga gtgtccccag aagcttataa caataagtcg ctgggggcct aattgcagct
    10261 aacggcctgc atcatgaaag acgacaggta aattatttgc cgcgcaccag aggtaagttt
    10321 tcgccagccc agccagcaac gccctctttc agcacgaata cttgcgcaaa accagctttc
    10381 gtcagcgcgt ttgcaggctc ctggcactgc atgccagaac cgtctaccac gataaccggt
    10441 ttgtctttgt gcttctcaag ctcaccaaca ttgttggctt tgatttcgct cggcaacagg
    10501 ttgatagaac ctgcgatatg gcctttacgg aagtcatcac gctgacgtaa atccacaacc
    10561 acagcatctt ctttgttgat cagacgcgta gcttcaccac gagtaatcac cttcactttc
    10621 gaggtcaggc tcttaaacgt agtcacaata accgccacca gtaacgcgat ccaggcgata
    10681 ctcagtatgg gatgacggcc aacaaattgc ataatttctt gcatgggggg taacaactcc
    10741 cgacgtagtg attaaaaaaa ccaggaaagg agtatacctg cgcggtgcgg caaatacagc
    10801 cagcgcgtta actggaatgc aattttgcgg ggcgcgacga aaaaaaagcg caaatctggc
    10861 atcggcttgc cgcgagcggt cgtatatttt gatcttcaga ggctatttta tcgattcagc
    10921 tgtagtaaaa ttacgcaaat tttgactctt gagtatgagg ttgtcgcaat gtcggtttct
    10981 aaaaaaccta tggtactggt gattctggat ggctatggct atcgtgaaga acagcaggat
    11041 aacgccattt ttagtgctaa aaccccggta atggatgcac tgtgggccaa tcgtccgcat
    11101 accctgatcg acgcttccgg tctggaagtc ggtctgcctg accgtcagat gggtaactcc
    11161 gaagtaggtc acgttaacct gggtgccggc cgcatcgtgt atcaggacct gactcgtctg
    11221 gacgttgaaa tcaaagatcg cgctttcttc gctaacccgg tactgactgg cgcggtagat
    11281 aaagcgaaaa acgcaggcaa agcagttcac attatgggtc tgctgtccgc aggcggcgta
    11341 cacagccacg aagatcacat catggcgatg gtagaactgg cagctgaacg cggcgcagaa
    11401 aaaatctacc tgcacgcatt ccttgacggt cgcgacactc cgccgcgcag tgctgaatcc
    11461 tcgctgaaaa aattcgaaga gaaatttgcc gcgctgggta aaggccgcgt agcgtccatc
    11521 attggtcgtt actacgccat ggaccgcgat aaccgttggg atcgcgttga aaaagcttat
    11581 gacctgctaa ctctggcgca gggtgagttc caggccgata ccgccgttgc tggtttgcag
    11641 gctgcttatg ctcgcgacga aaacgacgaa ttcgtgaaag cgaccgttat ccgtgcagaa
    11701 ggtcagccag acgcggcaat ggaagacggc gatgcgctga ttttcatgaa cttccgtgct
    11761 gaccgcgcac gtgaaatcac tcgtgcattc gtgaacgctg atttcgatgg cttcgcgcgt
    11821 aagaaagtgg ttaacgtcga tttcgtgatg ctgaccgaat acgccgctga catcaaaact
    11881 gcggttgctt acccaccagc ttccctggtt aacaccttcg gcgagtggat ggcgaaaaac
    11941 gacaaaactc agttgcgtat ttccgaaacc gaaaaatatg cccacgttac tttcttcttc
    12001 aacggtggcg tagaagagtc gttcaaaggc gaagatcgca ttctgatcaa ctcaccgaaa
    12061 gtggctacct acgatctgca accggaaatg agctccgcag agctgaccga aaaactggtt
    12121 gcggcaatca agagtggcaa atacgacacc attatctgta actatccgaa cggcgacatg
    12181 gtaggtcaca ccggggtgat ggaagcggcg gttaaagcgg ttgaagcgct ggatcactgc
    12241 gtggaagaag tcgcgaaagc ggttgagtcc gtgggtggac aactgctgat caccgctgac
    12301 cacggtaacg ctgaacagat gcgcgatccg gcaacgggtc aggcacacac ggcacacacc
    12361 aacctgccag ttccgctgat ttacgttggt gataagaacg tgaaagcggt tgaaggcggc
    12421 aaactttctg acatcgcgcc gaccatgttg tcgctgatgg gtatggaaat cccgcaagag
    12481 atgactggta agccgctgtt catcgtggaa taatccctcc ccatgagggg aaaggcgatt
    12541 aataccatga cacgggccgt gaaaccgcgc aggtttgcaa tcaggcccat catctacgcc
    12601 agcgttctta gcgctggcgt attgttgtgc gccttttccg cccacgcgga tgagcgtgac
    12661 caactcaaat ctattcaggc agatatcgcc gcgaaagagc gcgcggtacg ccaaaagcaa
    12721 caacaacgct ccagcctgct tgctcagttg aaaaagcagg aagaagcgat ctcggaagcc
    12781 acccgtaagc tgcgcgaaac gcaaaacacg ctcaatcaac tgaataaaca gattgatgag
    12841 atgaacgcgt cgattgccaa actggagcag caaaaagccg cccaggagcg cagcctcgcc
    12901 gcacaactgg atgccgcatt ccgtcagggc gagcataccg gtattcagct gattctcagc
    12961 ggtgaagaaa gccagcgtgg acagcgttta caggcttatt tcggctatct caaccaggcg
    13021 cgacaagaaa ccattgctca gttgaaacaa acgcgtgaag aagtcgctat gcaacgcgcc
    13081 gagctggaag agaaacagag cgagcaacaa acgctgttgt atgagcagcg cgcccaacag
    13141 gcgaagctga cccaggcgct gaacgaacgt aaaaagacgc tggcagggct ggagtcttcc
    13201 atccagcaag gtcagcaaca gttgagcgag ctgcgcgcca acgaatcccg tctgcgtaac
    13261 agcattgccc gtgcggaagc cgcggcgaaa gcgcgtgctg aacgagaagc gcgtgaggcc
    13321 caggcggttc gcgaccgcca gaaagaagcg acgcgcaaag gcaccaccta caaaccgacc
    13381 gaaagcgaaa aatcgctgat gtcccgaact ggtggcctgg gtgcgccgcg tggtcaggca
    13441 ttctggccag ttcgcgggcc gacgctgcat cgctatggcg agcagctaca gggtgaatta
    13501 cgctggaaag gaatggttat cggtgcctct gaaggtactg aagttaaagc aattgccgac
    13561 ggtcgggtga ttctggctga ctggctgcaa ggctacggtc tggtggtggt ggttgagcat
    13621 ggtaaaggcg acatgagtct ttacggctat aatcagagcg cactggtgag cgttggttcg
    13681 caggttcgcg cgggccagcc gattgcactg gtgggcagca gtggcggtca gggtcggcct
    13741 tcactctatt tcgaaattcg ccgccagggt caggcggtca atccacagcc gtggttggga
    13801 agataagttt tgtttccatt tcgtcgtaac gttcttgcat ttgccgctct gttggcgctc
    13861 tcctcccccg taattgctgg caaacttgcc atcgtcattg atgattttgg gtatcgcccg
    13921 cacaacgaaa accaggtgct ggcgatgcct tccgctatct ccgtcgctgt attacccgat
    13981 tcaccacacg ccagagagat ggcgaccaaa gcgcataaca gcggtcacga agtgttgatt
    14041 catctaccga tggcaccact gagtaaacag ccgctggaga aaaatacgct acgcccggag
    14101 atgagcagcg acgaaattga gcgcattatt cgtagtgcgg tcaataacgt gccctatgcc
    14161 gtggggatca acaaccacat gggcagcaag atgacctcta acctgtttgg tatgcagaaa
    14221 gtgatgcagg cgctggagcg ttacaatctt tacttcctcg acagcgtaac catcggtaat
    14281 acccaagcga tgcgcgccgc acaaggcact ggcgtgaagg tgatcaaacg gaaagtgttc
    14341 ctcgacgatt cgcaaaatga agcggacatc cgtgtgcagt tcaatcgcgc aattgacctc
    14401 gcgcgtcgca acggttcaac cattgccatt gggcatcctc acccttcaac ggtacgcgtg
    14461 ctacaacaga tggtttataa cctgccgcca gacattacgc tggtgaaagc cagcagcttg
    14521 ctgaatgaac cgcaggttga tatgtctaca ccgccgaaaa atactgtgcc cgacacaccg
    14581 cgtaatccgt tccgtggcgt gaagctctgc aaaccgaaga aaccgataga acctgtttat
    14641 gctaatcgct tctttgaagt attaagcgaa agcatcagcc agagcacgct gatcgtttac
    14701 ttccagcatc agtggcaagg ctggggcaaa cagcccgaag cggcaaaact taacgctagc
    14761 gcaaattaaa ggcgctacgc gtaatgcgat ggtgcgaccg cgttttgtcg cgccattgcc
    14821 ataagcggaa agaccacagt aacgcctggt aacccacctt cacgctgcgc acattagtaa
    14881 tcaggcgctt atacataccg gaagtaaata tctcggcaat catccgttgg cgggtaagaa
    14941 tatccggctc tttgcgcacc gcatggcaaa cacgcaatgc ttcgtaagtt atttgctgat
    15001 gaaattccgg ataaatcgta attttgtcgg cataatttcg atttaatttc tccagcaggc
    15061 gggtaatctt aatatagtga cgttgataat taaggttttt attcccttgt ctatgcaacc
    15121 gactcactga cgtattatgc agataatatt tatataatga ttgctcggta tatcgcgcac
    15181 gcagcgcatt aaacatgaat tctgttgtcc agacaatatc ctgatgatgt aatccggcaa
    15241 taaatttaat gttattttta acaataacat cgcggcgata aacccccatc cagacaacgt
    15301 gagtccaacg gcgggaagaa agccccatcc gcagccagtc cgggccggtt aatacaccgg
    15361 ttgagcgaag gcgatcgctg gggatggatt gccaggtttc tcccgtttca cgaaaacacc
    15421 agtcagcgtt gcactgcgcc acgtcgaggt cgtcctctaa cgccatggtc atcagcgttt
    15481 cgtacatggt gggatacacc tcatcgtcag catcgacaaa agcgacatat ttgcccgtcg
    15541 ccgcctcgat cccacgatta cgcgccaccg atgcgccagc attcgcctga tgcaacaaac
    15601 gaacgtgcgg atagttttct gcgtaatgct ttgctatttc aacagaatta tccgttgaac
    15661 catcgttaat aataatgatt tccagagcag tccaggtttg cgtaattaaa gattccatac
    15721 aagtgcggaa atcatcgccc gcattatata acggaataat aacactaagt ttattggtgc
    15781 tgttcatcat aaattaccga cgacctctga agttatcctg acgttttaca ggctttgtat
    15841 taagaaacta ttaagcctgt gagatattat ttttacaggt gagagagtca cttcagggta
    15901 gagagataat gagaacagct atataagaga tcagcgcaat tacgaactct ttattacact
    15961 gacaggattt tacatattta acggattatc ctcaggaaaa agggctggaa taccagccct
    16021 tttttgtgtt aatcccagct caggataact ttcccggact ggcccgaacg catggcgtca
    16081 aagcccttct ggaaatcatc gatagagaaa cgatgagtaa tgatcggcga aagatcgagg
    16141 ccagactgaa tcagcgccgc catcttgtac caggtttcaa acatctcacg accgtaaata
    16201 cctttaatga acaagccttt aaagatcact ttggtccagt cgatagacat atcagacggt
    16261 ggaataccca gcatcgcaat acgaccgccg tggttcatgg tgtcgagcat ggtacgaaac
    16321 gctggcggcg caccggacat ttccagaccg acatcaaagc cttcggtcat gcccagttct
    16381 gccatcacat cattgaggtt ctctttagcg acgttaaccg cacgggtgat acccatttta
    16441 cgcgccaact caaggcggta ttcgttaaca tcagtaatca ccacgttacg tgcaccaacg
    16501 tgtttcgcca ccgccgccgc cataatacca atcgggcctg caccagagac cagcacatcc
    16561 tcgcccacca gatcaaacga cagcgcggta tgcacggcgt taccgaaggg atcaaaaatg
    16621 gaagccagat catcggaaat attgtcgggg attttgaaag cgttgaacgc cggaatcacc
    16681 agatactcgg caaagcagcc cggacggtta acgccgacgc cgatcgtgtt gcggcacaga
    16741 tgggtacgac cgccacgaca gttgcgacag tgaccgcagg tgatatggcc ttcgccagaa
    16801 acgcgatcgc caatcttgaa acctttcact tcctgaccaa tacctaccac ttcaccgaca
    16861 tattcatggc ccacgaccat cggcaccggg atggtttttt gcgaccactc atcccagtta
    16921 tagatgtgaa cgtcagtccc gcagatggct gttttacgga ttttaatcag cagatcgtta
    16981 tgcccgagtt ccggtacagg aacgtcggtc atccagatgc cctcttccgc tttcagtttg
    17041 gataacgctt tcatctcaca tcctcaggcg ataacgccca gttgtttacc aatacgcgtg
    17101 aatgcttcta ctgcacgcgt aatttgctca ggggtatgcg ccgcagacat ctgggtacga
    17161 atacgcgcct gacctttcgg aacgaccgga tagaagaaac cggtgacgta aatcccctct
    17221 ttttgcagct cacgagcaaa tttctgcgct actaccgcat cgccaagcat gaccggaata
    17281 atggcgtgat cggctcccgc cagggtaaag cccgccgccg acatttgctc acggaactga
    17341 cgcgcgttcg cccacaggcg gtcacgcagt tcgctgcccg cttcgaccat ctccagcact
    17401 ttgatagacg cggcaacaat ggccggtgcc agcgagttgg agaacaggta cggacgagaa
    17461 cgctggcgca gccactcaac cacttctttg cgcgccgccg tgtagccgcc agaagcgcca
    17521 cccagcgctt taccgagcgt gccggtgata atatcgaccc ggcccatcac atcgcagtat
    17581 tcatgggaac cacgaccgtt ttcaccgaca aaacctaccg cgtgtgagtc atctaccatc
    17641 accagggcat catatttatc cgccagatcg caaacgcctt tcaggttggc aatcacgccg
    17701 tccatcgaga acacaccatc ggtggcaatc agcacatgac gcgcaccggc ttcacgcgct
    17761 tctttcagac gtgcttccag ctcctgcata tcgttgttgg cataacgata gcgtttagct
    17821 ttgcacagac gcacaccatc aataatagac gcgtggttca gtgcgtcgga gataatggcg
    17881 tcttccgcgc ccaacagcgt ttcgaacagg ccaccgttag catcaaagca ggaagagtag
    17941 agaatcgcat cttccatccc caggaaggcc gccagttttt gttcaagctc tttatggctg
    18001 tcctgagtgc cgcaaataaa acgcaccgaa gccatgccga aaccgtgaga atccattccc
    18061 gcctttgccg ccgcaatcag atccggatga ttcgccagtc cgagatagtt attggcacaa
    18121 aagttaatga cgtggcttcc atcagccaca gtgatatctg cttgctgcgc agacgttata
    18181 atgcgctctt ctttaaacaa cccttccgcc cgtgcggttt ccagatcgtt ggttaactgc
    18241 tgataaaatt ctccacgcat tgcgattctc cagacagggc aaattccagc acatattacc
    18301 caaacttata ggtcgcgacg agatgacgcg ttaacacttc tgcaaaattc aggataaata
    18361 acgtccatca atctcacttt tggcgaatgg ctgaagccaa cgccgtgtag tgatatgata
    18421 gaagtattcg tgtctgagat tgtctctgac tccataattc gaaggttaca gttatgatca
    18481 tcgttaccgg cggcgcgggc tttatcggca gcaacatcgt taaagccctg aatgataaag
    18541 gcatcaccga tattctggtg gtggacaacc tgaaagacgg caccaagttt gtgaacctgg
    18601 tggatctgga tatcgcggac tatatggata aggaagactt cctgatccag attatggctg
    18661 gcgaagagtt cggcgatgtc gaagcgattt tccacgaagg tgcgtgctct tccaccaccg
    18721 agtgggacgg caagtatatg atggataaca actatcaata ctccaaagag ctgctgcact
    18781 actgtctgga gcgcgaaatc ccgttcctgt atgcctcttc cgcagccacc tacggcggac
    18841 gcacctccga ctttattgaa tcccgcgagt acgaaaaacc gttgaatgtc tacggttact
    18901 caaaattcct gtttgatgaa tatgttcgtc aaatcctgcc agaagcgaac tcgcagattg
    18961 ttggcttccg ctatttcaac gtttatggac cgcgtgaagg ccataaaggc agcatggcga
    19021 gcgtcgcttt ccatctcaac actcagctta acaacggtga atcgccgaag ctgttcgaag
    19081 gtagcgagaa cttcaaacgc gacttcgttt acgtaggcga cgtggcagat gtaaacctgt
    19141 ggttcctgga aaatggcgtt tccggcatct tcaacctcgg tactggtcgt gcggaatcct
    19201 tccaggcggt agcagatgct acgcttgctt atcacaagaa aggccaaatc gaatacattc
    19261 cgttcccgga taaactgaaa ggccgctacc aggcgttcac gcaggcagat ctgacaaatc
    19321 tgcgcgcggc gggttacgac aaaccgttca aaaccgttgc cgaaggtgta acggaataca
    19381 tggcttggct gaatcgcgac gcataagagc tctgcatgaa aatactggtg atcggcccgt
    19441 cttgggttgg cgacatgatg atgtcgcaaa gtctctatcg cacgctccag gcgcgctatc
    19501 cccaggcgat aatcgacgtg atggcaccgg catggtgccg tccattatta tcgcggatgc
    19561 cggaagttaa cgaagctatt cctatgcctc tcggtcacgg agcgctggaa atcggcgaac
    19621 gccgcaaact gggtcatagt ctgcgtgaaa agcgctacga ccgcgcctac gtcttaccca
    19681 actccttcaa atccgcatta gtgcctttct tcgcgggtat tcctcatcgc accggctggc
    19741 gcggcgagat gcgctacggt ttacttaacg atgtacgcgt gctcgataaa gaagcctggc
    19801 cgctaatggt ggaacgctat gtcgcgctgg cctatgacaa aggcattatg cgtacagcac
    19861 aagatctgcc gcagccattg ttatggccgc agttgcaggt gagcgaaggt gaaaaatcat
    19921 atacctgtaa tcaattttcg ctttcatcag aacgtccgat gattggcttt tgcccgggtg
    19981 cggagtttgg tccggcaaaa cgctggccac actaccacta tgcggagctg gcaaagcagc
    20041 tgattgatga aggttatcag gtggttctgt ttggctcggc gaaagatcat gaagcgggca
    20101 atgagattct tgccgctttg aataccgagc agcaggcatg gtgccggaac ctggcggggg
    20161 aaacacagct tgatcaagcg gttatcctga ttgcagcctg taaagccatt gttactaacg
    20221 attctggtct gatgcacgtt gcggcggcgc tcaatcgtcc gctggttgcc ctgtatggtc
    20281 cgagtagccc ggacttcaca ccaccgctat cccataaagc gcgcgtgatc cgtctgatta
    20341 ccggctatca caaagtgcgt aaaggtgacg ctgcggaggg ttatcaccag agcttgatcg
    20401 acattactcc ccagcgcgta ctggaagaac tcaacgcgct attgttacaa gaggaagcct
    20461 gacggatgcg ggttttgatc gttaaaacat cgtcgatggg cgatgttctc catacgctgc
    20521 ccgcactcac tgatgcccag caggcaatcc cagggattaa gtttgactgg gtggtggaag
    20581 aagggttcgc acagattcct tcctggcacg ccgccgttga gcgagttatt cctgtggcaa
    20641 tacgtcgctg gcgtaaagcc tggttctcgg cccccataaa agctgaacgc aaagcgtttc
    20701 gtgaagcgct acaagcagag aactatgacg cagttatcga cgctcagggg ctggtaaaaa
    20761 gcgcggcgct ggtgacgcgt ctggcgcatg gcgtaaagca tggcatggac tggcaaaccg
    20821 ttcgagaacc gttagccagc ctgttttaca atcgtaagca tcatattgca aaacagcagc
    20881 acgccgtaga acgcacccgc gaactgtttg ccaaaagttt gggctatagc aaaccgcaaa
    20941 cccagggtga ttatgctatc gcacaacatt tcctgacgaa cctgcctaca gatgctggcg
    21001 aatatgccgt atttcttcat gcgacgaccc gtgatgataa acactggccg gaagaacact
    21061 ggcgagaatt gattggttta ctggctgatt caggaatacg gattaaactt ccgtggggcg
    21121 cgccgcatga ggaagaacgg gcgaaacgac tggcggaagg atttgcttat gttgaagtat
    21181 tgccgaagat gagtctggaa ggcgttgccc gcgtactggc cggggctaaa tttgtagtgt
    21241 cggtggatac ggggttaagc catttaacgg cggcactgga tagaccgaat atcacggttt
    21301 atggaccaac cgatccggga ttaattggcg gatatgggaa gaatcaggtg gtttgtaggg
    21361 ctccgctaat gaatttaaat aatttagaag cagcggctgt ttataaaaaa atcacattaa
    21421 tttaataaat agtaagtggg cattataccc acttacttag tcaacgcttt tattattgtg
    21481 gttcattttt actcaaaaac gatatagaca ggaagaggca cacaatccac gatgccggta
    21541 ttggtttagc atgtgtaatg acatcactaa tgccaaataa cataatagaa gcagggaaaa
    21601 ccaagagtat atattttttc tctcgtatac agtaatagat cattgcaaca tagaacataa
    21661 taagaacaag aagaccaatt tttcctttcg tggaaaga
//
LOCUS       DACBVI010000069        21663 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.069, whole genome shotgun sequence.
ACCESSION   DACBVI010000069 DACBVI010000000
VERSION     DACBVI010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.069"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            99..701
                     /locus_tag="IU325_12685"
     CDS             99..701
                     /locus_tag="IU325_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proQ/FINO family protein"
                     /protein_id="HAP5809877.1"
                     /translation="MGKNQEERKTPVIVVKKRRTFSLPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFSRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGINNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKWLRMQAQQENISGQ"
     gene            757..1251
                     /locus_tag="IU325_12690"
     CDS             757..1251
                     /locus_tag="IU325_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809878.1"
                     /translation="MCDSEAEKILFILNKHERAYRRLIPVYIFLAIVLWILPILLIVY
                     LYFCIVDNEFLRIGFCIAWTAYYFLSLFKYFVKYFFPLDREDLLKLMDLAKDSPDAKQ
                     ELLERLLSGKRMTGRDEMDIKRILKTKEALDNIRNFIDKQQLTNSDTPKKSTVPNEDK
                     KGIR"
     gene            complement(1525..1998)
                     /locus_tag="IU325_12695"
     CDS             complement(1525..1998)
                     /locus_tag="IU325_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809879.1"
                     /translation="MRDKTREAMRLFLGGRCYTAEKLEKDYLAEVANYSNDRWEAPQR
                     ASRLAASVKRYKTSEMLRFIFATIAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVF
                     GEKGRQHRSADSNPEMVEKMAARYRHAAELHWSATLAEIERVKRLYQTKIKKSKK"
     gene            complement(2115..2963)
                     /locus_tag="IU325_12700"
     CDS             complement(2115..2963)
                     /locus_tag="IU325_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HAP5809880.1"
                     /translation="MNNDELATRRAQAIAEDRCFSKGRLRDEFRMKPAPGAEPVKWYK
                     NTYGGRFAVYRIADCVPMREKRPLTSKQQLAGQRLSVLSRLNSTSGRMARQAYDWLSL
                     APLFLDTETTGLDNTAEALEIGLTDAAGQVVFETRLKPTVAIGAQAAAVHGISEQALC
                     GAPSWTDVARQLRHAIGDRPVIIFNARFDIRILKQTAAAHSDPADWLEELTVYCAMEL
                     AAGYYGATNRYGMISLACAASQAGLTWEGQAHSAIADARMTAGVVNAIAAYHLELLQE
                     QARLKI"
     gene            complement(3003..3284)
                     /locus_tag="IU325_12705"
     CDS             complement(3003..3284)
                     /locus_tag="IU325_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HAP5809881.1"
                     /translation="MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLA
                     GQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            complement(3281..3550)
                     /locus_tag="IU325_12710"
     CDS             complement(3281..3550)
                     /locus_tag="IU325_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809882.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVAAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK"
     gene            complement(3605..3763)
                     /locus_tag="IU325_12715"
     CDS             complement(3605..3763)
                     /locus_tag="IU325_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001700046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809883.1"
                     /translation="MTESGYGLAGLGMGLAKSINPYRLTPGFGGFTPVPYATTECRLP
                     CRWRGISW"
     gene            complement(4460..5491)
                     /locus_tag="IU325_12720"
     CDS             complement(4460..5491)
                     /locus_tag="IU325_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP5809884.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATTFLVEPERK
                     RTKRRRGEHSTKPKCENPSWYRPERYKALSGQLGHAYNRLVKKDPVTGEQSLRMHMSL
                     HPFYVQKRTYAGRKYAFRPEKQRLLDAVWPVLVSFSDAGTHTVGMSVSRLAREISPKD
                     SKGKVIPELEVTVSRLSRLLAEQVRFGVLGVSEETLWDRETRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHR
                     RAKAAASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRLAIRELRQLE
                     LTLAAPPPH"
     gene            6464..6727
                     /locus_tag="IU325_12725"
     CDS             6464..6727
                     /locus_tag="IU325_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809885.1"
                     /translation="MPYHKDRNLYFLSSSMVAQAGQALAWPVSDDAGIPTPVWAITNE
                     RRNSVIAFLVVIGDLCYVTSTDTETPGHHSVGRHVSRAINKPA"
     gene            6696..6932
                     /locus_tag="IU325_12730"
     CDS             6696..6932
                     /locus_tag="IU325_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HAP5809886.1"
                     /translation="MFPELSTNQLKVCVFYAMGVPYDAIAQNCRLSPETVRTYLKRSL
                     KNLNLEGYDALRSAVLMRTFVFMISNTAKKNEKM"
     gene            7374..7907
                     /locus_tag="IU325_12735"
     CDS             7374..7907
                     /locus_tag="IU325_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusG"
                     /protein_id="HAP5809887.1"
                     /translation="MNIEHLNNRNWYLAQYNTAGKNRESLFSWLNEQNVVPWTPLITR
                     KIRRADSRRCYRERIFAIFPGYFFILANFDIQPVSALRRHSAFIDFVKFGGEIKPVNK
                     DIVDGLMKIYPDPVLNPGAREELNAASSIWLTKAQYQYLLRMENTLQPESRISLLLEL
                     VSNAEHHGFMERLVNIP"
     gene            8160..8843
                     /locus_tag="IU325_12740"
     CDS             8160..8843
                     /locus_tag="IU325_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /protein_id="HAP5809888.1"
                     /translation="MTTTEHPVRQLLWCALNALKTAQENHNITSETGLRHYLLEWLSG
                     ASRHPEFRSLPGEIMALKALTEKDRNIPIIGTLNTLFLSSATVGECPLFRFRAALGKL
                     QKAGWRTYVCPWPERVFNESIERACSGKRHLLQLSRTEECFLPTGDMRAPVTLQLITP
                     LHRKTMQLLETAELIFSDEGFQVVRGYEHQFGIERRETLFHTLFIGLPSLPEDVWGAT
                     QEQTTITLH"
     gene            9016..9567
                     /locus_tag="IU325_12745"
     CDS             9016..9567
                     /locus_tag="IU325_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809889.1"
                     /translation="MKKQPLLWMTIAAALLSGCTGHREHHPQVTFTVDTVRTDTTASN
                     PICLAEKYGSLLNERHPDLFAGAQGSISRSAFFNDSLTLTPETLHGMIQNTPCVESLV
                     TSVGPDTFTADVSPGVRMQVSPDIHGAPGNITLRLSTTDQKRKQTVEQSISLRPEQSL
                     VVNGFTGDGAGEKRVVLITPHVR"
     gene            9729..9983
                     /locus_tag="IU325_12750"
                     /pseudo
     CDS             9729..9983
                     /locus_tag="IU325_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136193.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
     gene            10026..10478
                     /locus_tag="IU325_12755"
     CDS             10026..10478
                     /locus_tag="IU325_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pili assembly chaperone"
                     /protein_id="HAP5809890.1"
                     /translation="MSFLFKLTIAILIVFAILFPILISSDNKLSALFWKIPKDFWVFL
                     IIMYWAIITVVFIESSEQARIRRQQYLQQYKMPNVVHENVVIENSIISRETTDDESDI
                     NTLYLDSFKSKNNCKSFETNSNLLDKNGKPVEIMVCDVQELHFFVPKN"
     gene            11152..12219
                     /gene="pilL"
                     /locus_tag="IU325_12760"
     CDS             11152..12219
                     /gene="pilL"
                     /locus_tag="IU325_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis lipoprotein PilL"
                     /protein_id="HAP5809891.1"
                     /translation="MKKNCLTLILPSLLSGCAGISGSNNVAPSDTPALVFVDGQISES
                     AGVLALTQQRLSPPKITPPPRVSVPETVKTAQPVSESRPLPSPTISQNFSRLIITGKP
                     GSAPVTLSPTARNRTVSQWIKTLLPEGWKFRQENAATPKLNTKLVSWSANDQWTRSLN
                     RLLEEQHLWGHLDWNNKTLTVTTSAAAPAQDTATTISPAAMVTTSTYPESPTTANSQN
                     KPRNPFRGNSVSSSTPAATPTPTGSTVKSIPLMTGTPVKPVSQGKEWRAPAGTTLREN
                     IIKWAEETKCESMASTHWMVIWPLSVTDYRLDAPLMFRGSFESAMEQVFELYRTAQKP
                     LYAQASRMQCIITVSDTRDGR"
     gene            12219..12656
                     /gene="pilM"
                     /locus_tag="IU325_12765"
     CDS             12219..12656
                     /gene="pilM"
                     /locus_tag="IU325_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HAP5809892.1"
                     /translation="MGWLVMAVGLTILIITGSYQNQKMSETTNAQQYASASVWASQIL
                     MIANRINDIRYVSGQQDGVISSDKLALPVTPDSRIKHQLQQGRLWVWMPEQPGLVETL
                     RSKSRGSALIGIFQNGQLTWLSGTATGLTPPAGITAGSVVYVN"
     gene            12670..14352
                     /locus_tag="IU325_12770"
     CDS             12670..14352
                     /locus_tag="IU325_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HAP5809893.1"
                     /translation="MKKSHQRSMKLAVLPCMIAVALSISGCTFSEINKMQKKAQEDSA
                     HAREKVSALSARKSQALTWLDNQWINPVPVAQVSREKKQTAPACYITQARKGEITLQE
                     LGQRITAVCGIPVIITPDAANSTLEGGATRQMTGTLPAPDENGRLPLSSLGSTTMTTS
                     TQPLTLNNLMWQGDINGLLDLMASRSGLYWRMDNGRIVFYLTETRTYPLHMLNTKTSS
                     SSSVSSGSTSTMGATGGQDNSASGDATSSQSTTVGQEYDLYEDIRKTIEAMLTPEKGR
                     YWLSASSSTLTVTDTPAVQEAVARYVDEQNSIMNRQVALNVQVLSVSNTRNEQFGLDW
                     NLVYKSLHSAGATLNNASGDFTGATSAGVSILDTATGNAAKFSGSSLLIKALSEQGDV
                     SVVTSQESTVTNLTPVPIQMADQTVYVAQSATTTTTDVGATTTLTPGMITTGFNMTLL
                     PLIQKTGNLQLQMNFNLSDPPTIRSFTSKDGNSYIEMPYTKLRSLSQKVNLKEGQSLV
                     VTGFDQNNTTTSKAGTFTPANPLFGGSQTGKNERSTLVIIITPTFPSGGNNG"
     gene            14345..15640
                     /gene="pilO2"
                     /locus_tag="IU325_12775"
     CDS             14345..15640
                     /gene="pilO2"
                     /locus_tag="IU325_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HAP5809894.1"
                     /translation="MADEDINPVVLLADPKVNHRVWAACLKWSPVVKKQRVPSHQKHK
                     PHVKSRRLTSLKVTVGSRSSRGKISRITGTGILARPERNHYFSLALAFCSWVRNGYGV
                     FRYSDKELLFLASINGQPAVMADLSGNDADVAQKVSLFLTMNEEPPEKWQVVSPLEHP
                     DNWESIITRLSSADLRRCKLTVGNRSKFTLPAVLFLVAAGAGTVFWMTQPEPDVVPTP
                     EEIAARARLQFKKPDPPPELPHPWASQPVISDFLKACADLRKPSPVALEGWKLTGGTC
                     TPETFTLIYERQPGGTIEGFLARSKEIFNVIPDFNLKDGARLASVTRPLPSLPRRDEA
                     VPTPSEQLMRVFTWFQKKQLTPAINEIAIPEPLPGNDGEPAPVQKWKEYQFSLSTPVN
                     PDELFPLFQDTGVRLSNIHFELNGGTFSYSSEGHIYASR"
     gene            15627..16079
                     /gene="pilP"
                     /locus_tag="IU325_12780"
     CDS             15627..16079
                     /gene="pilP"
                     /locus_tag="IU325_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HAP5809895.1"
                     /translation="MRPGKLLIIPSIFFFSGFSFATTQPLVTIGELEAQQNRNILLQA
                     KVQGAQLQKQLEESDVTSSSETVSGFSGVTASLPSVSEQPTSKNRKELPIIMEINGKD
                     KRLNAVLRMADGRQTSVTTGSQLPGTSVTVKSISLSGVTLSDGTTLTF"
     gene            16090..17643
                     /gene="cpaF"
                     /locus_tag="IU325_12785"
     CDS             16090..17643
                     /gene="cpaF"
                     /locus_tag="IU325_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP5809896.1"
                     /translation="MDKYNLKDALLFISRGDTHEILIETNQRTRPDVQSNLQELLKLY
                     PDINPKVVSLSELQEAGQDEKNRGPKERIIHLKDLADVSESEKKVLSYFETARKLGAS
                     DIHFLISESIFKVRMRIFGELQTVDEDQPALGYSLCATAILSMADVTETSFFPQREQD
                     ARLSPQLMRKIGIFGARYSHRPTGDGLIAVMRLIPDDGDKVPTFKQLGFIPEQIRLLN
                     IMLRRPEGKIVLSGPTGSGKSTTLRSACRVYLDDNQGRHLLTIEDPLEGQILGATQTP
                     IICDKSDEDAVKLAWSRAISSAMRLDPDAIMEGEMRDLISMMSTTYAAQTGHIVLTTL
                     HTNSALGIPERMITMGMNADLICDAQLLIGMISQRLVPTLCPSCRIPWEKRAPELSDD
                     ERDYLERHCNKDSLCSTDNIWFRNHHGCSECNHDVIINGRKRGEIGKGLTGRTVIAEV
                     IEPDNRLFQILKTRGKVAARKYWLENMKGISRVEHLLRRINEGLVDPLEADRIIPLDE
                     DERLSIDDV"
     gene            17656..18753
                     /locus_tag="IU325_12790"
     CDS             17656..18753
                     /locus_tag="IU325_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001699973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilR"
                     /protein_id="HAP5809897.1"
                     /translation="MREMNFSQRLRRFIVRKTFSAPYRVQFYEALRFLLENKQPLKTA
                     LEQMRDAWTDFGRKWHPFAELATDCIESLRENSGENSLEYTLSLWVPQEEAAVISAGI
                     RSGSIVDALQFATTLTDAKEQIHQAIWQMAIYPVGLLIMMTGTLYVLNTELIPELSKI
                     SSPDSWSGALGFLYGLSVFVDNYGAICAVLFAVITGLISWSLPNWKSPDSVRTFADKI
                     MPWSIYQDIQGATFLLNMAALLKAKMTTLNSLNILQEFASPWLSTRLDSIIYRVRQGD
                     HLGLALRQCGYQFPSREAANFLSLLQGDGATELISNYGQRWLSQTLQRVKKRANVIRL
                     IMLIFLVMSLMLLVFAIMDIQSISDNSMGNF"
     gene            18771..19385
                     /locus_tag="IU325_12795"
     CDS             18771..19385
                     /locus_tag="IU325_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilX"
                     /protein_id="HAP5809898.1"
                     /translation="MLVENINTTLTGNNKKNEPHDKGWAILEQGTIALVVLFVIVVVL
                     GSLYALRTRANVATETANIQTIITSAQSLLKGSDGYTFTSSAKMTGALIQMGVIPSGM
                     TVQGDKTSGTATLYNAWGGAVTVAPASTSGFNNGFTVTYDKVPQDACIQIATRISKTG
                     LTNGITLNSTAHSDGKVTTEEASTQCKADNGSTGTNKLIFTING"
     gene            19395..19955
                     /locus_tag="IU325_12800"
     CDS             19395..19955
                     /locus_tag="IU325_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP5809899.1"
                     /translation="MSGGTVPLRLISLILLFSLIPPARASYVAPKQIWTNKWDNCFAA
                     AGARYQIEPLLLKAISAGESSLRPGAININKDKKTGKASSTDYGLMQINSTHIPKLIN
                     MGVIKKSEDLITKPCLNIHIGSWILARHFQICGVSWNCLGSYNAGFRKDRHETREQYA
                     NKIWRIYRDMKGICFPGQGGRQCRQS"
     gene            19940..20596
                     /locus_tag="IU325_12805"
     CDS             19940..20596
                     /locus_tag="IU325_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP5809900.1"
                     /translation="MQTIVGISLSPVYILPLMFTFSIIGRTLLFRVRYFLSDTGHLWY
                     KTHPAVLSGIWLYSIAVALIILSSSPLLYRIHAVLILSFILQMAVTDALTGLLPGTFT
                     RRFLIAGMLSQITTDIWWFRTTEFATAAIVLFCLHKLVNRHRLNIGTGDLWLIAGITA
                     WSGLYNAIWCVLLGTGGFVLWHSTWCIKGHKEGPLGPWLCFGHVLLLLDNLYQPLWVI
                     "
     gene            20596..>21663
                     /gene="pilV"
                     /locus_tag="IU325_12810"
     CDS             20596..>21663
                     /gene="pilV"
                     /locus_tag="IU325_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HAP5809901.1"
                     /translation="MKKYDRGWASLETGAALLIVMLLIAWGAGIWQDYIQTKGWQTEA
                     RLVSNWTSAARSYIGKNYTTLQGSSTTTTPAVITTTMLKNTGFLSSGFTETNSEGQRL
                     QAYVVRNAQNPELLQAMVVSSGGTPYPVKALIQMAKDITTGLGGYIQDGKTATGALRS
                     WSVALSNYGAKSGNGHIAVLLSTDELSGAAEDTDRLYRFQVNGRPDLNKMHTAIDMGS
                     NNLNNVGAVNAQTGNFSGNVNGVNGTFSGQVKGNSGNFDVNVTAGGDIRSNNGWLITR
                     NSKGWLNETHGGGFYMSDGSWVRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQLERT
                     AVAGASCSPNGLVGRDNTGAIL"
BASE COUNT         5843 a         5315 c         4979 g         5526 t
ORIGIN      
        1 aacggactta ttaaccatga aaaatcacct gacggaaaat ttactaattt atggattgtg
       61 atattattaa agagtaaaac cagccagccg aggtatttat gggaaaaaat caggaagaaa
      121 ggaaaactcc cgtcattgtg gtgaaaaaac gtcgtacttt ttcgctacca tccttatccg
      181 aaaaaacaga tattatcgcc cctgtattta ctgaacagac ggcagaatca gcccccgccg
      241 gaataaattc atctgccgtc gaaacacata tccccgaagc gccagccagg aagaagaaaa
      301 agaaaagaca ccgtttttca cgtccctccc actggacccg tgagtatact cacgagtgcg
      361 tggaaaaaat aaaggccctg tttcctcatc ttcgtgctga aggtggtgga tttattcccc
      421 tgaaaattgg cataaacaat gatatttcgg catttctggc agaacacccg gagacagaac
      481 tgaccatgga cgaatggctc tgtgcagtgt cctgcatcac atcccgccgc gtatatctgc
      541 agcggacagc tgtcgccggc gtcccacgtt atggcctgga tggtcacccc aaagggcagg
      601 tctcagactc tgaagcacag agtgccggtc gcagactggc gacattagaa caaaagtggc
      661 tcaggatgca ggcacagcag gagaatattt ctggacaata aacactcctg ataacaggtg
      721 gacattacgt atcagaaaaa atgggaagag aatgttatgt gtgattcaga agcggagaag
      781 attttattca tcctgaataa acatgaacgg gcatacagac gcctgatccc cgtatatatt
      841 tttttagcta tagtattatg gattttacct attctcctga ttgtatacct gtatttctgt
      901 atagttgaca atgagtttct cagaatcggt ttctgtattg catggacagc atactacttt
      961 ctttcgcttt tcaagtattt tgtaaaatac ttctttcctc tcgatcgtga agatttgctt
     1021 aaactgatgg atctcgccaa agattctcct gatgcgaaac aggagcttct tgagcgtctt
     1081 ttgtccggaa aaagaatgac cgggcgggat gaaatggata tcaagcgcat tctaaaaaca
     1141 aaggaagcac ttgataacat caggaatttt attgataaac agcagttaac gaatagtgat
     1201 actccgaaga aaagcacggt cccaaatgaa gataaaaaag gcataagatg atgatcacag
     1261 gaaatggaat aaacactgta actgtcaatg gaaaagtgaa acacatcaca gaactggatg
     1321 atataacact ttgtttaagc agcgccggga gcagcattat aaaatcatgt acagagttgc
     1381 gtgacgacat gataagttct cattctcaat atgttcttta ccagattata ccagtgatac
     1441 tttaacgcac catcattaat gctaatgtat gcatacatta gcattaatga tgaaggagaa
     1501 gaacaacaat ggcagtcaac ccacttattt tttggatttc ttaattttcg tctgataaag
     1561 ccgctttacc cgttcaattt ctgccagcgt tgcggaccag tgtagttcgg cggcatgacg
     1621 atatctggca gccatttttt ccaccatctc cggattgctg tccgcgctgc ggtgctgacg
     1681 ccccttctcc ccaaaaactt caacaaccag ccactggctt ccggttctgc cgaacaatgc
     1741 cttgcacaaa cgtctcacag tcagcggggt taaatctgga tcgggatcgt aggcaatggt
     1801 agcaaaaata aagcgcagca tttctgatgt cttgtaacgc tttacgctgg cagccaggcg
     1861 tgaggcacgt tgcggtgctt cccagcggtc gttactgtaa ttagccactt cagccagata
     1921 atccttttca agtttttcag ccgtgtagca cctgccgcct aaaaaaagcc gcatggcctc
     1981 cctggttttg tctctcacat atcatccctc attaatgcta atgtacacat acattagcat
     2041 taacatgctg acagcaacaa gcggcggatt tacgtctgga taacgtaaat gattgcggta
     2101 tacaggccag gcggtcagat tttcagccgt gcctgttcct gcagcagttc cagatgatat
     2161 gcagcaatag cgtttaccac ccctgccgtc atccgtgcgt cggcgatcgc tgagtgtgcc
     2221 tgcccttccc aggtcagtcc ggcctggctg gcagcacagg ccagtgaaat catgccatag
     2281 cggttggtgg ccccataata tcctgcagcc agctccatcg cacaatatac cgtcagttct
     2341 tccagccagt cagccggatc gctatgtgcg gcagcagtct gtttcagaat gcggatgtcg
     2401 aaccgggcat taaagataat tactggtcgg tccccgattg catgacgcag ttgccgcgcc
     2461 acatctgtcc atgatggtgc gccgcagaga gcctgctcgc tgataccatg cacagcggcg
     2521 gcctgcgccc cgatggcgac cgtgggcttc agcctggttt caaataccac ctgaccggcc
     2581 gcatcggtca ggccgatttc cagggcttcc gcggtattat ccagtcctgt cgtttcagta
     2641 tccagaaaga gcggtgccag actcagccag tcatatgcct gccgtgccat ccggccactg
     2701 gtgctgttaa gtctggataa aacggaaagt cgctggccag ccagctgttg tttactcgtc
     2761 agagggcgtt tctcacgcat cggaacacag tctgcaattc ggtatacagc gaacctgcca
     2821 ccataagtgt ttttatacca cttaaccggc tcagcaccgg gggctggttt cattctgaat
     2881 tcatcgcgca gacgtccttt cgagaaacac ctgtcttcgg caatcgcctg cgcacgtctg
     2941 gtggccagct catcattatt catggttatt gcatctcctt tccttatatc cggacagccc
     3001 ctctatggcc acttctgtgc agtatgcaac acgcgcagga tatacacttt tccccactgg
     3061 ctgtcgacct cataaaccag aacaaaatgc gggtgaataa ccagttcccg ggtaccttcc
     3121 acacgaccgt tgcggccttt atacggctgc ccggccaggc tgctggctga ataaccaatc
     3181 cgttcgtcca gttcaatggc ggccatcagg ttctgctccg caatgtactc acgaatgcgc
     3241 ttcctgtcct ggctggccag catggtccag aatatctcca tcatttaccg gcgaccttcc
     3301 tgctcatttc atcccgccat gcagcagcag aagattcgac catgtcatgg ggaagcacat
     3361 cgccgcgctc gagttgctga cgtcctgccg caacctggtc acggaaccag gtattatgct
     3421 gccgttcaac ggtctggcgc ataaaatccc ggattaactg agagccatta cggtccatgc
     3481 tttttgcagc ggccataaaa gcatccttca gttcagcgtc gattcttaaa ctcatattaa
     3541 cctgtgccat ttatcacctc gtcattacta cgtatataca cagtatataa cgattcagat
     3601 atcgtcacca ggatatgccg cgccagcggc atggaaggcg gcactccgtt gtagcatatg
     3661 gcacaggagt aaaaccgccg aagcccggcg taagccggta cggattgata gattttgcca
     3721 atcccatccc cagccctgct aggccatatc cgctttcagt catgacagag cttttgtgcg
     3781 cggtcggagt ggtcccgaca agggtttacc cgcagtcggg gcgtatctcc gcgttagcgg
     3841 gccgtcaggg ccgcttacga gcgtgtactg aggacttcca gccagaagac tgacagcgat
     3901 gatgctgctt agttacaaca ttcataatta aaagcgatgt ggttcccgcc cttcgggcgg
     3961 gggcggcctc gcttttcagt tatgaggggt gggcacggtg ctgcgtttgt gtggtgtttt
     4021 ttattcctga tgttttcgcg gtggtttcag aacggtgagg ttcggggccc gcgcgttagc
     4081 gtgggaggtg cttttagatt ctgagcggcg cggccttggc cgctgcacgc gaaggcctct
     4141 ttgtgtttgt tcacagtggt ttcagagata aagactttcc ctgcggcgaa gcaacgcagg
     4201 ggattaaatg atggggttaa gactatattg caggtttgct gtttcaggtt tgcagcttct
     4261 taaagttctt tgtttgcgga tttatgtgga taactgaaac aacgtggcca cgttctgatg
     4321 actgatgcgt ggttttatga gcaaatatgc ccctctgagg ggatttaagg tgattactgc
     4381 ggattttggc ctgagcgata cctgcgccga aagccccgga ggggctggcg atgattcagt
     4441 gctgagggaa tggtgctgtc tagtgcggtg gcggggcagc cagcgtcagt tcaagctgac
     4501 gcaactccct gatggccagt cgcttcagat ggtcatcgga acaaaaatag gcttcatccg
     4561 gcggcagacg cttcctgaga tactctgcca tctcatgaat ctgctggtcg gcaggcagtt
     4621 tcttcaggcg tctggcgcgc ttactggctg ccgcttttgc acgacggtgt ttcagtgcat
     4681 cctggctgcg ctgcagatac cagcgttttc tggccgcatg tacggagata tcttcatcct
     4741 cacgcagaac accttcccga atgagctgct ggcggatttc actttcacgc agtcgtttct
     4801 gctgctgttc gtgaagtttt accatgtcga cgcccagcat ctgccagcct gccggtgtta
     4861 tccagacgta acgtggcaga cgctggcggg tttcacggtc ccacagggtt tcctctgaaa
     4921 cacccagcac accaaaacgt acctgttcgg ccagcaaacg ggaaaggcgg gagaccgtca
     4981 cttccagttc cggaataacc ttccccttgc tgtctttcgg gctgatttct ctggccagac
     5041 gggaaacact catgcctacg gtatgtgtgc ccgcatcact gaagctgacc agaaccggcc
     5101 agacggcatc gaggaggcgt tgtttttccg gacggaaagc atatttgcga ccggcatacg
     5161 ttcgtttctg cacgtaaaaa ggatgcagag acatgtgcat gcgcaggctc tgctcgccgg
     5221 tcaccgggtc ctttttcacc agacggttgt aggcgtgccc gagctgcccg ctcagcgcct
     5281 tatagcgctc aggacgatac caggacggat tttcgcattt gggtttggtg gagtgctcac
     5341 cacgacgacg cttcgtgcgc ttcctttccg gttcaaccag gaaagtggtc gcccttcccg
     5401 cttcatagtc ttcccagaaa cggatctgct cttcgggtgc taactgactc cagtgtactg
     5461 cgttatatgg agtattcttc agacctgcca caagctgtcg cttatggcgg agcgattgtg
     5521 ccgtgtattt atacactgca cagggttgaa tctctggtac ggtttcatta tactttaccc
     5581 gtattctgtg aggcccccac tatctttcgt cgttccgcca agttcgtaag aaagatagtg
     5641 ggggttttcc ttttatggct tccggaacat cttcagtctt ctgacttact gcccgtacat
     5701 gccatctctc ctgaatgcct tatggcgcat tctgccgtcc gactttcgcc gctttgctcc
     5761 acagagtgcc accctctgca cagctaataa cagccgaaat atacccgctt tacgttaatt
     5821 tgaaaagcat tacaacgacc tcttctcttc cagtgcctcc tcactgatac ctctaataag
     5881 ttaatcttga cctgcgtcaa aaaaataaaa tctattttta aaagcagatt tttctgcttt
     5941 agatccttct tttggattaa aagatcgact tttgtcctac tcacgatcat ttatcttaca
     6001 accgatccac ctaacagaat aatattctgt tttaatgcaa ttaaacgatc taaaaaccct
     6061 ttaaaaaatc attttgtaga tcattcgatc gtaaatagaa taattcttag atgataaaac
     6121 catttgttac acaacccgcc aattgtgtaa caacagcctc tacaagagca tatttgttca
     6181 tatgttcgtc agaactgcgg attgtctgga cgagggtcag acaccgtttc gtgcatatgg
     6241 accagcatgt tcgaacgcca caaagcagaa gcatcacgcc tggatcgctt cggcgcggag
     6301 cgttaagttt tgacgctttt ttgtatgttt tttaagctgt tagttgactc ggtgcgttat
     6361 actaagtacc ctttactggt cacaggcaaa actgtgatcg ggcgtaggaa cccgcgtagt
     6421 catcgtcggc gcagaagtta atttcttcat ttggcgtcgt tttttgccat accataaaga
     6481 caggaacttg tatttcttgt cgtcatctat ggtagctcag gcggggcagg cattagcctg
     6541 gccggtttcc gatgacgccg gtattcctac ccccgtctgg gctatcacca atgagcgtag
     6601 gaactcagtg atagcgtttt tagttgtcat cggagacctt tgctatgtca cctcaactga
     6661 tacagaaact cccggccatc actctgttgg aaggcatgtt tcccgagcta tcaacaaacc
     6721 agcttaaagt ctgcgttttt tacgcaatgg gtgttcctta cgacgccatt gctcagaact
     6781 gtcggctttc tcccgaaacg gtacgaacct acctgaagcg aagcctgaag aatttaaatc
     6841 tggaaggata tgatgcactt cgctctgctg tgctgatgcg aactttcgtt ttcatgatta
     6901 gcaatacagc taaaaaaaac gaaaaaatgt gaacaggtgt ccccattaaa ggggacactt
     6961 tcatggaaat tgcattttgc actttacagg cgatcccaaa ttgataaaca taaacaccaa
     7021 gcacaagtta ctttaataaa gcaaatcact tcagacggtg tcccagttat ttgtatctgg
     7081 ctcatgcccg atatatggcg atcttactct gaactgacgt gctgatgttt tatttcgcgt
     7141 atcacaatac tgtgccactg tattgctgat attagtgctt agccgttctt tatctgtgag
     7201 ttcgccaaat caccgataca gaacgtacta gagtctgctg gttatatgcc tgtcagaact
     7261 gcagtttatc cagatatggg ctggacactg cttcagacat atgatttagc atattcgcct
     7321 gacatcgcaa aatcgtccat catggatcgg ttttgcgcgg agcgttgaac ttcgtgaata
     7381 tagaacacct gaataaccgg aactggtatc ttgcgcaata taatacagcc ggcaaaaaca
     7441 gagaatcttt attctcatgg ctgaatgagc aaaacgtcgt accatggact ccattaataa
     7501 caagaaagat aagacgggca gactccaggc gctgttaccg tgaaagaata ttcgctattt
     7561 ttccagggta cttttttatt ctggctaatt ttgacattca accagtatcg gcattacgtc
     7621 gccatagcgc cttcattgat ttcgtgaagt ttggcggtga aataaaaccc gtcaataaag
     7681 atattgttga tggattaatg aagatttacc cggaccctgt gctaaatccg ggagccaggg
     7741 aagagctgaa tgcggcttcc agcatatggc tgacaaaagc acaatatcag tatctcctca
     7801 gaatggaaaa tactctccag cctgagtcga ggatatccct gctcctggaa ctggtatcaa
     7861 atgccgaaca tcacggcttt atggagcgcc ttgtaaacat cccatagcca gtatttcccg
     7921 gccttcgggc cgggcttttt tcaattcaga gaatattctg gagtgaagcc tgcacgaagc
     7981 catccccacc atggactccc cctgtctttt gatggaatcg tgtatttctt aatggcgaaa
     8041 gaaataaaag gcttcacccc agagtattga ttcgttttat caaaggccgg cccgtgccat
     8101 cacgtgtggc ggagagagcg gataccggtc ttcttttcat ctcacagtag gacattcgca
     8161 tgaccacgac agaacatccc gtgcgccagt tgctctggtg tgcactgaat gccctgaaaa
     8221 cggcacagga aaaccacaat ataaccagtg aaaccggatt acggcattac ctgcttgaat
     8281 ggctttctgg tgccagcaga catcctgagt tccgtagtct ccctggcgaa atcatggcgt
     8341 taaaagcatt gaccgaaaaa gatcgcaata ttcccatcat cggaacgctg aatacgctct
     8401 ttctcagctc tgccactgtc ggggaatgtc cgttgtttcg ttttcgggct gcactgggta
     8461 aattacagaa agcaggctgg cgcacatatg tctgcccatg gcctgagagg gtgttcaatg
     8521 aatccattga acgggcctgt tccgggaaac gtcatctgct acaactaagt cgcaccgaag
     8581 agtgttttct accgacaggc gacatgaggg caccagttac gttgcagctg atcactccac
     8641 tacaccgcaa aactatgcag cttctggaaa cggcagagtt aatattctcc gacgaaggat
     8701 ttcaggtggt gagaggatac gaacatcaat tcgggatcga acgcagagaa accctctttc
     8761 acaccctatt tatcgggctg cccagcctgc cagaagatgt ctggggtgca acacaggagc
     8821 agacgactat cacattacac tgaggatgtg taatccccgg cataccccgt ttccagtctt
     8881 cgcagcctgt accgcccttc ctgccgtttc cgacattctt tctgtcacta ctcgcttgtg
     8941 ccattttttc gcaagcggcc cccatcgaca tgacggtgcg agtctttctt tttaccctga
     9001 gaggaaatta acagcatgaa aaaacaacca ctactctgga tgacgatagc cgccgcactg
     9061 ctgtccggct gcacaggcca cagggaacat cacccgcagg tgacattcac ggtcgatacc
     9121 gtgcgaacag acacaacggc cagcaaccca atctgcctgg cggaaaaata cggaagcctt
     9181 cttaatgagc gccatccgga cctgtttgcc ggcgctcagg ggagtatttc acgcagtgca
     9241 ttttttaatg acagcctgac gctgacacct gaaacgctcc acgggatgat acagaatacg
     9301 ccctgtgttg aatcactggt aacgtccgta ggtccggata ccttcactgc agatgtctct
     9361 cccggtgtca gaatgcaggt atcaccggat attcacggtg cgcccggaaa tatcactctg
     9421 cgactctcga ccacagacca gaaacgaaaa cagactgttg aacaaagcat ttctctcaga
     9481 ccggaacagt cccttgtcgt gaatggattc accggagacg gagctggcga aaagcgcgtc
     9541 gttctcatca caccgcacgt gcgttaattc ccaacacaac ccgtttcccg tcttcgcagc
     9601 ctgtaccgcc ctttctgccg tttccggaat tctttctgtc accactcgct tgtgccgttt
     9661 tttcgcaacc ggtacgtgca gttacgacgg tacagctgct tcttttcccg ttccgaaagg
     9721 agtctttgat gccacagcaa catcccggat gactacaggt actggtcgtc gacacgcact
     9781 gcaaacgcaa gctgttttca acgaaaacac agacagatcc cgatgagctt gcccgacgct
     9841 tctgcacgcc ggataactgc cttgtggtgg tactatgcaa caaccgattt ctgttccgcc
     9901 tggaacgtgc acccagtagc cactgccgct ggcgcaaggg tagccgctcc cgccatcagc
     9961 acctgcagga ctggctgtcg taaaaacaac aaaaacaaaa gacctcgtca aaacaggagg
    10021 ggaccatgtc gtttttattt aaattaacca tcgcgatttt gattgtcttt gcaatccttt
    10081 ttccgatttt aatttcatca gataacaaac tgtccgcact tttttggaag ataccaaaag
    10141 atttctgggt tttcctgatt atcatgtact gggcaatcat cacggtggtg ttcatagaaa
    10201 gcagtgaaca ggcacgcatc cgccgccaac aatatttgca acagtacaaa atgccaaatg
    10261 tggtacatga aaatgtggtg atagaaaaca gtattatttc aagagaaaca acagatgacg
    10321 aatctgacat taacaccctg taccttgatt catttaaaag taaaaacaac tgcaaatctt
    10381 ttgaaaccaa ttcaaattta ctggataaaa atggtaagcc tgtggaaata atggtgtgtg
    10441 atgttcagga actgcatttt ttcgttccaa agaattaaac agttaatccc ccttactctg
    10501 ttatcttagt cgccggcatc aactaacatg aaaaataata acgtaccatt atatctatgg
    10561 ttactttggt gtacgttttg cctgtacatc actaccccgt ggcaatactt ccgttatcta
    10621 tacactgacc acatatcact atcttatata ttatatttct gtatagtggt tattatttgc
    10681 ttttccccac tacttttcag atgcgtgctt cttatcgtaa ggtctgtatc taaatacaac
    10741 ttcacattct ttgcgaaaaa aaatggcagt aaagaatcaa gcatacatct tagcccctat
    10801 gccgatacac gttgcccgga gtcaatgagg aaacactggg atgtgttact ttctctgata
    10861 tcaaaagcaa ttaataatga tgaaaccatt gttatgcatt cacatctctt tactcctgca
    10921 agaataaata aacttataaa gagactggaa tcttatggtt tgaatttctc tgttattaca
    10981 tgcccacgga aaacaagtct gtatgaaaaa ttatcaattc cttttgtata tctcttattt
    11041 cagtggtctc tcccgcattt gcattcggac ggaatgacag tcgtcattcg tcctctccag
    11101 ccgcctcaca ccaattaaca aacacgtata tacgaacacg gagtctccgt tatgaaaaaa
    11161 aactgtctga cacttatact gccgtcatta ttgtccggct gtgccggaat ttccggcagc
    11221 aataacgtgg caccatcaga tacgcctgct ctggtttttg ttgacggaca aatcagtgaa
    11281 agtgctggcg ttctggcact tacgcaacaa cgtctttctc cacctaaaat aactcccccc
    11341 ccgagagtca gcgtgccaga gacagtaaaa acagcacagc ctgttagcga atcccgaccg
    11401 ttaccttcac caacaataag tcagaatttc tcccggttaa tcattacagg caagcctggt
    11461 agcgcccctg taacactatc gccgacggcc cgtaaccgga ccgtcagtca gtggataaaa
    11521 acattactac cggaaggatg gaaattcaga caagagaatg cagccacgcc caagctgaat
    11581 accaaactgg tttcatggtc tgccaatgat cagtggaccc gttctctgaa ccggttgctg
    11641 gaagaacaac acctctgggg ccatctggac tggaataata aaacgttgac cgtcacaaca
    11701 tcagctgctg caccggcaca ggacacggca accactatct cgccagcagc catggtaacc
    11761 acatccactt accctgaatc tccaacgaca gcaaatagcc aaaacaaacc acgaaatcca
    11821 ttcaggggaa acagcgtatc ttcgtcaacg cccgccgcca ccccgactcc aaccggctca
    11881 acagtcaagt ctatcccgct catgaccgga acgcctgtta aaccggtatc tcagggtaaa
    11941 gagtggcgtg cgccggcagg caccacatta agagagaaca ttatcaagtg ggcagaagaa
    12001 acaaaatgcg aaagcatggc atcaacgcac tggatggtca tatggccact ttctgtaacc
    12061 gattaccgtc tggatgcgcc tttaatgttc cggggatcat ttgaatcagc catggagcag
    12121 gtatttgagt tatatcggac tgcgcaaaaa cctttatacg cacaggcttc tcgcatgcag
    12181 tgcatcatta cagtttctga tacaagggat ggtcgctaat gggatggctg gtcatggccg
    12241 tcggcctgac cattctgatt atcactggta gctaccagaa tcagaagatg tctgaaacca
    12301 caaacgccca gcaatatgca agtgcgtctg tatgggcaag tcagatacta atgattgcta
    12361 acagaatcaa tgatatcaga tacgtatcag gccagcagga cggagtaatt tcctcagata
    12421 aactggctct ccctgtcact ccggactccc gaattaaaca tcagttacaa caggggcgtc
    12481 tgtgggtctg gatgcctgaa caaccaggtc ttgttgaaac tttacgttca aaaagtcgtg
    12541 gttctgcact aatagggatc ttccaaaacg ggcaactcac ctggctatca ggaactgcaa
    12601 ccggtctgac acccccggca ggcattactg ctggttctgt cgtctacgtt aactaatctg
    12661 gatatattca tgaaaaaatc acaccagcgt tcaatgaagc tggcggtgct cccctgcatg
    12721 atcgccgtcg ctctttccat ctccggatgt actttcagcg aaatcaacaa aatgcagaaa
    12781 aaagcacagg aagactcagc acatgcacgg gaaaaggtat cagccctttc ggcccgtaaa
    12841 tcgcaggctc ttacctggct cgataatcaa tggataaacc ctgttccggt cgctcaggta
    12901 tcaagagaga aaaaacaaac agctccggcc tgctacatca cgcaggcaag aaaaggagag
    12961 atcactctgc aggaactggg gcaacgtatt actgccgtat gcggcatccc tgtgatcatc
    13021 acgcctgacg cagccaattc aactcttgaa ggaggcgcta cccgccagat gacaggaaca
    13081 ctaccagcac cagatgaaaa tgggcgtcta ccgttaagca gcctgggcag cacaacaatg
    13141 actacctcca ctcagccatt aacgctgaat aacctcatgt ggcagggaga tatcaatggt
    13201 cttctggatc tgatggccag ccggagtggt ctgtactggc gcatggataa tggtcggatt
    13261 gtattctatc tgactgaaac cagaacgtat ccacttcata tgctgaacac caaaaccagc
    13321 agcagttcca gtgtcagctc tggctcaaca agcacaatgg gggcaacagg aggccaggat
    13381 aactcagcat ccggtgatgc aacgtcctct cagagcacaa ccgttggtca ggaatacgat
    13441 ctgtatgaag acatccggaa aactattgaa gcaatgctga caccagaaaa aggccgttac
    13501 tggttatctg catcgagctc aacgctgact gtcactgata ctccagctgt ccaggaagcc
    13561 gtcgcacgat atgtggacga acaaaacagt attatgaacc gccaggtagc cctgaacgta
    13621 caggttctga gcgtcagcaa taccagaaac gaacagttcg gtctggactg gaaccttgtt
    13681 tataaatcgc tacattccgc cggagcaacg ttgaacaatg caagcggaga ttttacaggc
    13741 gctacatctg caggcgtatc aattctggat acggcaacag ggaatgccgc caaattcagc
    13801 ggttccagtc ttctgattaa agcgctgagt gaacagggcg atgtcagtgt tgtgacttca
    13861 caagaaagca ctgtcacaaa cctgacgccg gtacctatcc agatggcaga tcagacggtt
    13921 tacgtcgccc agtcagcaac aacaacgact acggatgtag gagcaacaac aacattaacg
    13981 ccgggcatga tcaccaccgg attcaatatg accctgctgc ctttaattca gaaaacgggc
    14041 aatctccagt tgcagatgaa ttttaatctg tcagatcccc caacaatccg tagctttacg
    14101 tcaaaagacg gaaacagtta catcgaaatg ccgtatacca aactgcgttc actgagccag
    14161 aaggtcaatc tgaaagaagg gcaatcactt gtcgttactg gtttcgatca gaacaatacg
    14221 acgacaagta aagccggtac gtttacgcca gcaaatccat tatttggtgg ttcacaaacc
    14281 gggaaaaatg aacgcagcac gcttgtaatc atcattaccc cgactttccc gtcaggaggc
    14341 aacaatggct gatgaagata tcaacccggt tgtgcttctc gcagatccta aagtaaatca
    14401 tcgcgtgtgg gcagcctgtc ttaaatggag tcctgtcgta aaaaaacagc gagtcccttc
    14461 tcatcagaaa cacaagcccc atgtaaaatc cagacggctt acctctctga aagtgactgt
    14521 aggcagcaga agttcccgtg ggaagatcag ccgcattacc ggcacaggaa tactggccag
    14581 gccggagcgc aatcattatt tttcccttgc tctggctttc tgctcatggg tgagaaatgg
    14641 atacggcgtc ttccggtaca gcgataagga gcttttattc cttgcctcca ttaatggcca
    14701 gcctgccgtt atggctgatc tgtcaggaaa tgatgctgat gttgctcaaa aggtcagtct
    14761 ttttctgacg atgaacgaag agccccccga aaaatggcag gtcgtatctc ctctggaaca
    14821 tcctgataac tgggaaagta ttattacccg gttatcatca gcagatctcc gccgttgcaa
    14881 acttactgtc ggcaatcgca gcaaattcac actgcctgcc gtactgtttt tagtagcagc
    14941 cggcgcaggc actgtattct ggatgactca gccagagccg gatgtcgtac cgacgcccga
    15001 ggagatagct gcacgggcaa gattgcagtt taaaaagccc gatccaccgc cagaactccc
    15061 gcatccatgg gcatcacaac ctgtaatcag tgattttctg aaagcctgcg cggaccttcg
    15121 aaaaccatca ccggtcgcgc tagaaggctg gaaacttacc ggtggaacat gtactcccga
    15181 gacattcaca ctgatttacg aacgacagcc aggaggaact attgaaggct ttctggcaag
    15241 aagcaaagaa atctttaatg ttattcctga ctttaacctt aaagatggtg cacgcctggc
    15301 ttctgtaacc cggccactgc cttccctgcc ccgcagggat gaggcagtcc ctaccccctc
    15361 agaacaactg atgcgggtgt tcacgtggtt tcagaaaaaa cagttaacgc cggccattaa
    15421 tgaaatagcc ataccggaac cattaccagg taacgatggt gaaccagccc ctgttcagaa
    15481 atggaaagaa tatcagttca gtttatcaac gcctgtaaat cctgatgagt tgttcccttt
    15541 atttcaggat accggcgttc gtctgagcaa tatccatttt gaattaaacg gcggtacttt
    15601 cagttattcc tcagaaggtc atatttatgc gtccaggtaa attactcatt attccatcca
    15661 tatttttctt ctccggtttt tcatttgcaa caacacaacc tcttgtcaca ataggagaac
    15721 ttgaagcaca acaaaaccgg aatattcttt tacaggcaaa agttcagggc gcacagctac
    15781 agaaacaact cgaagaatca gatgttactt catcatctga aactgtttca ggtttctctg
    15841 gtgtgactgc ctctttgcca tcagtttcag agcagccgac atctaaaaac agaaaggaat
    15901 tgcccatcat tatggaaata aacggcaaag ataaacgtct taatgccgtt cttcgcatgg
    15961 ctgatggacg tcagacaagc gtcaccactg gcagccagct tccaggcaca tccgtcacag
    16021 tgaagtcgat ctcattgtct ggcgtcacgt taagtgatgg caccaccctt actttctgag
    16081 taacgtgata tggataaata taatctgaag gatgctctcc tttttatatc caggggagac
    16141 acccatgaaa ttcttataga aacaaaccag cggacaaggc ctgacgtaca aagtaatctc
    16201 caggagctgc taaaattata tccggatatc aacccaaaag tagtctcgtt gtcggagtta
    16261 caggaggcag gtcaggatga gaaaaacaga ggcccgaaag aacgaattat tcatctgaaa
    16321 gaccttgctg atgtcagtga aagtgagaag aaagttctat cttattttga aactgccaga
    16381 aaacttggtg cttcagatat tcatttctta atttcagagt ctattttcaa agttcgcatg
    16441 agaatattcg gtgaactgca gactgtagat gaagatcaac cagcactggg ctattcactt
    16501 tgcgcaacag ccattctgtc aatggctgat gtcactgaaa ccagtttctt ccctcaaagg
    16561 gagcaggatg cgcgcctgtc cccacaatta atgcgaaaaa taggaatatt tggtgcacgc
    16621 tacagtcaca gacctacggg agacggtctg attgcagtta tgcgcctgat acccgatgac
    16681 ggagataaag ttcccacgtt taaacaactg ggctttatcc cggaacaaat ccggttgctg
    16741 aacataatgc tgcgccgccc ggaaggaaaa atagtcctgt caggtccaac cggttccggt
    16801 aaatcgacaa cgttacgcag cgcctgccgt gtatatcttg acgataatca ggggcgacac
    16861 ctgctaacaa tcgaagatcc gctggaagga cagatactgg gagcaacaca gactccgatt
    16921 atctgcgaca aatccgacga agatgccgtc aaactggcat ggagtcgggc tatttcatcg
    16981 gcaatgcgac ttgaccctga tgccatcatg gaaggggaaa tgcgcgatct tatctcgatg
    17041 atgtcaacca cctacgcggc acagaccggg cacatagttc tgacgacatt acatacaaac
    17101 tcagctctgg gtataccgga gcgaatgatt acgatgggta tgaatgccga tcttatttgc
    17161 gatgcgcaat tactgattgg gatgatcagc cagcgccttg taccaactct gtgtccctcc
    17221 tgccgtattc catgggaaaa gcgagcacct gaacttagcg acgacgagcg tgattacctt
    17281 gagcggcact gcaataaaga ttcactctgc agtacagata atatctggtt ccgaaatcat
    17341 cacggatgct ctgaatgtaa tcacgacgta atcattaacg gcagaaagcg cggtgaaata
    17401 ggtaaaggcc tcaccggcag gactgtaatt gctgaggtga tagaacctga taaccgctta
    17461 tttcagattc tgaaaacacg cggcaaagtt gcagcacgta aatactggct ggaaaacatg
    17521 aaaggcatca gtcgtgtaga gcatcttctt cgccgtatca atgaaggtct ggtagaccct
    17581 cttgaagccg acagaatcat ccctctggac gaagacgagc gcctcagcat cgatgatgtc
    17641 taacaggaga acaacatgcg tgaaatgaat ttcagtcaac ggctgaggcg tttcattgtc
    17701 agaaaaacgt tcagcgcccc ctacagggtg cagttttacg aagccctgcg ttttctgctg
    17761 gaaaataaac agccattaaa gaccgcactg gaacagatgc gggatgcctg gacagacttt
    17821 ggacgaaaat ggcatccctt tgctgaactg gccacagact gcattgagtc tcttcgtgaa
    17881 aacagtggcg aaaactctct ggaatatact cttagtttat gggttcctca ggaagaggcc
    17941 gcagtaatca gcgccgggat aagaagtggt tcaattgtgg atgcactgca attcgccacc
    18001 actcttacag atgcaaaaga acaaatccat caggctatat ggcaaatggc tatttatccg
    18061 gtgggattgt taatcatgat gacggggaca ttatatgttc tgaatacaga gctaatacct
    18121 gaactgagca aaataagctc ccccgattca tggagtggtg ctcttggttt tctttatgga
    18181 ttatctgttt ttgtcgataa ttacggcgct atatgtgccg ttctttttgc agtgatcaca
    18241 ggtttaatat catggtctct gccaaactgg aaaagccctg acagtgttcg tacttttgcc
    18301 gacaagataa tgccatggag tatttatcag gatatacagg gagcaacatt tctcctgaat
    18361 atggctgcac ttctgaaagc caaaatgacg acgctcaact cgctaaacat tcttcaggag
    18421 tttgcatcac catggttaag cacacgtctt gacagcatta tttaccgcgt ccgccagggg
    18481 gatcatcttg gactggcttt gcgtcagtgc ggttatcagt tcccatccag ggaagcagcc
    18541 aacttcctgt ctttattaca gggagatggc gcaacagagc taatcagcaa ttatggtcaa
    18601 cgatggctta gtcaaacact tcaacgtgta aagaaacggg caaacgtgat tcgtttaatt
    18661 atgctgatat ttcttgtcat gtctctcatg ttactggttt ttgccattat ggatattcag
    18721 tctattagtg acaacagcat gggtaacttt taaacaaaac gaggatattt atgttagtag
    18781 aaaatattaa taccacttta actggaaata ataagaagaa tgagcctcat gataaaggat
    18841 gggcaatttt ggagcaggga acaatagcgc tcgttgttct ttttgttata gtcgttgttc
    18901 tgggtagttt gtatgcgttg cgtacaagag ccaatgtggc aactgaaacc gcaaatatac
    18961 aaacaattat cacaagtgcc cagagcctgc taaaaggtag cgatggatat acatttacca
    19021 gcagtgccaa aatgacaggt gccctcattc agatgggtgt cattccctct gggatgaccg
    19081 tacagggcga caaaacatct ggtacagcaa cgctctataa cgcatggggc ggtgccgtaa
    19141 ctgttgcccc ggcttcaaca tcagggttca acaacggttt taccgttacc tatgataaag
    19201 tcccacagga tgcctgtatc cagattgcca cacgaatcag taaaactggt ttaactaacg
    19261 gcattaccct gaacagtact gctcacagtg atggaaaagt cactactgaa gaagccagta
    19321 cgcaatgcaa ggcagataat ggcagcactg gtacaaacaa acttatcttc accatcaatg
    19381 gttaaggagt tttaatgtca gggggtacag tacccctcag acttatttca ttaatcctgc
    19441 ttttttctct tattccaccc gcccgagctt catacgtagc tcccaaacag atctggacca
    19501 acaagtggga caattgcttt gccgctgccg gcgcgcgcta tcaaattgaa ccattattat
    19561 tgaaggctat ttctgccggg gaatcttcgt tgaggccggg cgcaattaat ataaataagg
    19621 acaaaaaaac aggtaaagcc agtagcacgg attatgggtt gatgcaaata aactcaaccc
    19681 atattccaaa gctgataaat atgggcgtaa taaaaaagtc tgaggattta attaccaaac
    19741 catgccttaa tatccatata ggaagctgga tactggcaag acattttcag atatgtggcg
    19801 tcagctggaa ttgccttggg tcatacaatg ccggattccg taaggacaga catgaaacaa
    19861 gagagcaata tgccaataaa atatggagaa tttatcgtga catgaaaggg atatgttttc
    19921 ccggacaggg aggcaggcaa tgcagacaat cgtaggcata agtctatccc ctgtttatat
    19981 actgccattg atgttcacat tttccattat tggacgaact ctgctattca gagttcgtta
    20041 ttttttatct gatacgggac atctctggta taagacacac cctgctgttc tgtcaggaat
    20101 atggctatac agtatagctg tggcgttaat tattttatca tcatcaccac tgctctaccg
    20161 tattcatgct gtccttattc tttcgttcat actgcaaatg gcggtaacgg atgcactgac
    20221 aggtttatta ccaggaacat tcacccgacg ttttttaatt gcaggaatgc tatcacaaat
    20281 cacgactgat atatggtggt tccgtacaac agaatttgcc actgctgcaa ttgtcttatt
    20341 ctgccttcat aaattagtta accgccatcg tcttaatatt ggcactggag acctgtggct
    20401 tattgcaggt attaccgcat ggtcagggtt atataacgcc atatggtgtg tgttgctggg
    20461 cacaggagga tttgtcttat ggcattcaac atggtgcata aaagggcata aagaaggccc
    20521 tctgggtcca tggctatgtt ttggccatgt ccttttgctg ctggataatc tttatcaacc
    20581 actatgggta atctgatgaa aaaatatgac cgaggctggg catcgctgga aactggcgcg
    20641 gctttactga ttgttatgct cctgattgcc tggggagcgg gcatatggca ggactatatt
    20701 caaacaaaag gatggcaaac tgaagcacgt cttgtcagca actggaccag tgcggcccgc
    20761 tcttatatag ggaaaaacta cacgactctc cagggcagta gtaccacaac aactcctgcc
    20821 gttatcacga caaccatgct gaaaaatacc ggctttttgt ccagcgggtt tactgagaca
    20881 aacagcgagg ggcagcggtt acaggcatat gtggttcgaa acgcccaaaa cccggaatta
    20941 ctacaggcaa tggttgtatc cagtggtggc acaccttatc cagtgaaagc acttatccag
    21001 atggctaagg atattaccac tggtcttggt ggatatatcc aggacggcaa aacagccaca
    21061 ggtgcattac gttcctggtc agtagcttta agtaattatg gtgccaaaag cggtaacggg
    21121 catattgccg tattgttatc gacagatgaa cttagtggtg cagctgagga cactgatcgt
    21181 ctttacagat tccaggtcaa tggtcgccct gacttaaaca aaatgcacac ggccattgat
    21241 atgggatcaa ataacctgaa taacgttggg gcagtaaatg cccagacagg taatttcagc
    21301 ggcaatgtga atggtgtaaa tggcactttc agcggtcagg taaaaggcaa tagcggaaac
    21361 tttgacgtaa atgtcaccgc tggcggtgat atcagaagta ataatggttg gttaattact
    21421 cgtaacagta aaggctggct caatgaaact cacggtggcg gattttatat gtccgatgga
    21481 tcatgggttc gaagtgtaaa caacaagggc atctataccg gcggtcaggt gaaaggcggt
    21541 actgttcggg ctgatggtcg cctttatact ggtgaatact tacaactgga aagaactgcc
    21601 gttgctggcg catcatgttc gcctaacggc cttgtaggcc gcgataatac aggggcaata
    21661 ctt
//
LOCUS       DACBVI010000070        21326 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.070, whole genome shotgun sequence.
ACCESSION   DACBVI010000070 DACBVI010000000
VERSION     DACBVI010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.070"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            20..445
                     /locus_tag="IU325_12815"
     CDS             20..445
                     /locus_tag="IU325_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEc46 family
                     transposase"
                     /protein_id="HAP5809902.1"
                     /translation="MSNHDDLLAGFLRKRHSVSKLVVHLIFTTKYRRKLFDSQMIAQL
                     REAFGSAAAKLECEIIEMDGEPDHVHLLVAYPPKLAVSVMVNNLKSVSSRLLRQQNAH
                     LRMQSKTGLLWSRSYFVCSTGEATIETLRAYVQSQSTPD"
     gene            602..2662
                     /gene="ptrB"
                     /locus_tag="IU325_12820"
     CDS             602..2662
                     /gene="ptrB"
                     /locus_tag="IU325_12820"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HAP5809903.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPED"
     gene            complement(2659..3321)
                     /gene="exoX"
                     /locus_tag="IU325_12825"
     CDS             complement(2659..3321)
                     /gene="exoX"
                     /locus_tag="IU325_12825"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HAP5809904.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGHPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(3345..4001)
                     /gene="yobB"
                     /locus_tag="IU325_12830"
     CDS             complement(3345..4001)
                     /gene="yobB"
                     /locus_tag="IU325_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-N hydrolase family protein YobB"
                     /protein_id="HAP5809905.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASTRR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADLGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(4103..4333)
                     /locus_tag="IU325_12835"
     CDS             complement(4103..4333)
                     /locus_tag="IU325_12835"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HAP5809906.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            4472..4846
                     /gene="yobA"
                     /locus_tag="IU325_12840"
     CDS             4472..4846
                     /gene="yobA"
                     /locus_tag="IU325_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HAP5809907.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            4850..5722
                     /gene="copD"
                     /locus_tag="IU325_12845"
     CDS             4850..5722
                     /gene="copD"
                     /locus_tag="IU325_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HAP5809908.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLMFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            5735..6076
                     /locus_tag="IU325_12850"
     CDS             5735..6076
                     /locus_tag="IU325_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2511 domain-containing protein"
                     /protein_id="HAP5809909.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            6472..7128
                     /gene="pphA"
                     /locus_tag="IU325_12855"
     CDS             6472..7128
                     /gene="pphA"
                     /locus_tag="IU325_12855"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HAP5809910.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCGFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHIIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLSERQKVQGITGADHFWFGHTPLRHRVDIGNLHYIDTGVVFGGELTVVQLQ
                     "
     gene            complement(7129..7404)
                     /locus_tag="IU325_12860"
     CDS             complement(7129..7404)
                     /locus_tag="IU325_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HAP5809911.1"
                     /translation="MAGYLSWLFPRCKISPKLNGTAPHFGDEMFALVLFVCYLDGGCE
                     DIVVDVYNTEQQCLYSMSDQRIRHGGCFPIEDFIDGFWRPAQEYGDF"
     gene            complement(7425..7661)
                     /locus_tag="IU325_12865"
     CDS             complement(7425..7661)
                     /locus_tag="IU325_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HAP5809912.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(7779..9218)
                     /gene="rsmF"
                     /locus_tag="IU325_12870"
     CDS             complement(7779..9218)
                     /gene="rsmF"
                     /locus_tag="IU325_12870"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HAP5809913.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTASYGWTLTPIPWCEEGFWIERDDEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGIRNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCRWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPVLPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYSWQHEAVIALASPDNVNAFELTAQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(9298..11931)
                     /gene="yebT"
                     /locus_tag="IU325_12875"
     CDS             complement(9298..11931)
                     /gene="yebT"
                     /locus_tag="IU325_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HAP5809914.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(11900..13183)
                     /gene="yebS"
                     /locus_tag="IU325_12880"
     CDS             complement(11900..13183)
                     /gene="yebS"
                     /locus_tag="IU325_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HAP5809915.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            13313..13810
                     /locus_tag="IU325_12885"
     CDS             13313..13810
                     /locus_tag="IU325_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP5809916.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     TSNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            13907..14605
                     /gene="proQ"
                     /locus_tag="IU325_12890"
     CDS             13907..14605
                     /gene="proQ"
                     /locus_tag="IU325_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HAP5809917.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAVGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            14625..16673
                     /locus_tag="IU325_12895"
     CDS             14625..16673
                     /locus_tag="IU325_12895"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HAP5809918.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSKGGGALTEAVSLSGLFIPSGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            16865..17746
                     /gene="htpX"
                     /locus_tag="IU325_12900"
     CDS             16865..17746
                     /gene="htpX"
                     /locus_tag="IU325_12900"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HAP5809919.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(17792..19165)
                     /locus_tag="IU325_12905"
     CDS             complement(17792..19165)
                     /locus_tag="IU325_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5809920.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIGAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            19342..20133
                     /gene="kdgR"
                     /locus_tag="IU325_12910"
     CDS             19342..20133
                     /gene="kdgR"
                     /locus_tag="IU325_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HAP5809921.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(20277..20516)
                     /locus_tag="IU325_12915"
     CDS             complement(20277..20516)
                     /locus_tag="IU325_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809922.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            20675..20818
                     /gene="mgrB"
                     /locus_tag="IU325_12920"
     CDS             20675..20818
                     /gene="mgrB"
                     /locus_tag="IU325_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HAP5809923.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            20893..21180
                     /locus_tag="IU325_12925"
     CDS             20893..21180
                     /locus_tag="IU325_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809924.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLFIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
BASE COUNT         5516 a         5193 c         5445 g         5172 t
ORIGIN      
        1 ctttaggtaa atatatcaat tgagtaatca tgatgattta ctggcgggat ttctccgcaa
       61 acgacacagt gtcagtaagc tggttgtgca ccttattttt acgacaaagt atcgacgcaa
      121 actattcgac agtcagatga tcgctcagtt gcgtgaggct tttggctccg ctgcggcaaa
      181 gcttgaatgc gaaattattg agatggacgg ggagccagac catgtacacc tgctggttgc
      241 ctacccgcca aaactggcgg tcagtgtgat ggtcaataat ttaaaatcgg tatcatcacg
      301 tttgctacgc cagcagaacg cgcatttacg catgcagagt aaaacggggc ttttgtggtc
      361 gagatcgtac tttgtctgta gcaccggaga ggcaacgatt gaaacgctca gagcctacgt
      421 tcagagtcag tcaacacctg attgatcttt aaagccctgc gggcttttcg ccttatatcc
      481 ccgcccgcat tgggcgaggg tttacggcgt ttttcgctaa gcaatgacta ccctgtttcg
      541 cttgcatctc cggtgagttt tgccaccctt ataagatgtt tcaaccagaa agaacaataa
      601 catgctacca aaagccgccc gcattcccca cgccatgacg cttcatggcg atacgcgcat
      661 cgataattac tactggctgc gggacgatac gcgttctcag ccggaagtcc tggactacct
      721 gcaacaagaa aatagttacg gtcatcgggt gatggcctcg caacaagcct tgcaggatcg
      781 catcttaaag gaaatcatcg accgcattcc gcaaagagaa gtttctgcgc cctacataaa
      841 aaatggctac cgctatcggc atatttatga accaggctgt gaatatgcta tctaccagcg
      901 tcaatcggca ttcagtgaag agtgggatga gtgggaaata ttgctcgatg ccaataagcg
      961 cgcagctcat agtgagtttt attcgatggg cggaatggcg attacgcccg ataacaccat
     1021 tatggcgctg gcagaagatt ttctttcccg acgccagtac ggcattcgtt ttcgtaatct
     1081 ggaaacaggt aactggtatc cggaactgct ggataacgtt gaacccagct ttgtctgggc
     1141 aaatgattcc tggactttct actatgttcg caagcatccg gtgacgctgc ttccttatca
     1201 ggtctggcgt cacgccatcg gtacgccagc atcgcaagat acactgatct acgaagaaaa
     1261 agacgatacc tattatgtca gcctgcataa aacgacgtcg aagcactatg tagtcattca
     1321 tctggccagc gccaccacca gtgaagttcg cctgctggac gcggaaatgg ccgatgccga
     1381 gccgtttgtt tttctgccgc gccgcaaaga tcacgaatac agccttgatc actaccagca
     1441 tcggttttat ctgcgttcca accgccacgg caaaaacttt ggcttatacc gtacccgtat
     1501 gcgtgatgag caacagtggg aagagttaat tccgccacgc gataacatta tgctggaagg
     1561 gtttacgctg tttaccgact ggctggtggt tgaagagcgt cagcgcgggt taaccagttt
     1621 gcgccaaatc aaccgcaaga cccgggaagt cattggcatt gcctttgatg atccggccta
     1681 tgtgacctgg attgcctaca atccagaacc tgaaaccgcg cgattgcgtt atggttattc
     1741 ttccatgacc acaccagaca ctttgtttga actggatatg gataccggtg agcgtcgtgt
     1801 attaaaacaa acagaagtgc ccggttttga tgcggcgaat taccgcagtg aacacctgtg
     1861 gatagtcgcc cgtgatggcg tcgaagttcc ggtttcgctg gtctatcatc gcaaacattt
     1921 tcgcaaagga cacaacccat tgctggtgta tggctatggt tcttacggcg caagtattga
     1981 tgccgatttc agttttagcc gcttgagttt gttagatcgt ggctttgtct acgccattgt
     2041 ccatgttcgc ggcggtggtg agctggggca acaatggtac gaagacggaa aattcctgaa
     2101 gaagaaaaat acgtttaatg attatcttga tgcctgcgat gcattgttaa aactgggcta
     2161 tggctctcct tcgctctgtt atgcgatggg cgggagtgcg gggggcatgt tgatgggcgt
     2221 tgcaattaat caacgcccgg aattgttcca cggcgttatc gcccaggtac cgtttgttga
     2281 tgttgtaaca acaatgcttg atgaatcaat tcctcttacc actggtgagt ttgaagagtg
     2341 ggggaatccg caggatccgc aatattacga gtacatgaaa agctacagcc cgtatgacaa
     2401 cgtcaccgca caggcttatc cgcatttact ggtaacgacc ggtttacacg attctcaggt
     2461 gcaatattgg gaaccggcaa aatgggtcgc taaattgcgc gagctgaaaa ccgataacca
     2521 tcttttattg ctctgtaccg acatggactc aggccatggc ggtaaatctg gccgctttaa
     2581 atcgtacgaa ggcgtagcga tggaatatgc ttttctggtc gcgctggcgc agggaacatt
     2641 acccgctacg cctgaggatt aagtattttc cagataatgt ttcagtgtta aacgcagctc
     2701 cgggctcatg ctgtccaggt tattaaacaa ccagcgcaga tagcccggat cgcgttcggc
     2761 aacgtcggaa accgctttgc cacggtattt gccaaaggtg aaggtcgtca tcaacgacgg
     2821 atgtccggtg atatcggcca tctgttctgc cgtccagccg gaggtgttca taatatcgat
     2881 aagcaacgca gcggtgatat aacagtcata caacgcgcgg tgatgatgca ggcccggcgg
     2941 cgtctgtaca ttgagcttcc gtgttttata taacgccata ttgctgtact tgatccctgg
     3001 ccacaaacga cgggccagtt tcatagtgca aatccactca ccgggcatct caggcagtac
     3061 gcggcggtca aagctggcgt tgtgcgcgac ataccattca ctaccgtaat agtgcgggat
     3121 cacatcttca atccacggtt tatcggcgac catggcttcg gtgatgcgat gaatcgccat
     3181 cgcctgtgga ctaataggac gatcggggcg caccaggtgg ctcatggggt tgacgatttt
     3241 tccgtcaatg acatcaacag aggcaatctc aacgatccct ccctgcaaac cgcaggtttc
     3301 tgtatcgata atgcgcaaca tggcatgctc caggccgaaa aagcctagcg taatggaatg
     3361 atatcgcctt gccaaccctg tgatgaacgc tgaccgacta acaataatga accaagatcg
     3421 gcgcgaacga tgagacgacc atgttcatcc catagtgcac tgttaccacg ggcatttgcc
     3481 atcagcacgg caattgagta ctgatgtgaa aaaaattgta agcggcgggt agatgccagt
     3541 aggtcgggtt cccccagaca ttgccctgtc gtaaacagtg aacaggtcgg atccatatcc
     3601 ataccttccg gttgttcatc gaccaccgtg atggttctgg aacgacggcc cagacatgca
     3661 ccatgactct gatggtagat ccctggtgtt tttcgccacg gtgcgaacac cgcaattcca
     3721 cgaataaagc gatcgttata ttcaacggga aggccggcaa tgatggtcat tcgccaggtc
     3781 gttgcagcat aacaaagcgg gtctaacagc gagagatcgg gtggggcagg aagggcacgt
     3841 cgcgaataat cacaccccag taaagaaagc gatggaaaaa ctaacagctg acattgttgc
     3901 ctggcggcgg ccctcacaaa ctccagatga tgagctacct gttcggtcag tgaggtttta
     3961 cgaggctcat attgcgctgc tgcaactttc cagaacgaca taatgacttc cttttcaaaa
     4021 tgactgcaaa ttatgggtgc ggtttagaaa atactcataa tttagcaggc gttatgtaag
     4081 aaagtgtaac tctgtataag ttttatttaa gtttgggctc gtaaggtaaa cgtgacagat
     4141 tgaccgaagc caaacggtgg gcgataagcc gctcgcgaaa ccagctgcgc aaatgttcag
     4201 gctgttcacg ttcaaccgct tcagcgatca ccggcatatt gtagcgttct ttaaatgcca
     4261 ccccggccgc cgccaaatcg acattcactt tatccatttc tgtttgatcc agtttagcca
     4321 gattcttcag cattcttaat ctcctcatca ttcgcgggca aaagttacta agagtgagcc
     4381 tggatggcaa gccggaattg atgatcctcg actcagacat tagacaaggt tatgctctgt
     4441 aaaaaagtta ttacaaaaag gaatagataa tatggcttca actgcacgct cccttcgcta
     4501 cgcgctggca atcctgacca cttcgttggt gacaccttcc gtctgggcac atgcgcattt
     4561 aacgcatcag tatcctgcgg caaatgcgca agtgacagct gccccgcagg caatcacttt
     4621 aaacttctcg gaaggcgttg aaacaggatt cagtggtgca aaaatcacgg ggccaaaaaa
     4681 cgaaaatatt aaaacattac cggcgaagcg gaatgagcag gaccaaaagc aactgattgt
     4741 tccactggcc gattcgctga aacccggaac ctacaccgtt gactggcatg ttgtttcggt
     4801 ggacgggcat aaaacgaaag gacactacac ctttagcgtg aaataaatca tgctggcgtt
     4861 tacctggatc gcgctgcgat ttatccattt cacctcgctg atgctggtct ttggcttcgc
     4921 aatgtacggt gcctggctgg cccccttaac gattcgtcgc ttgctgacga agcgttttct
     4981 gcgtttacaa cagcacgctg ccgtctggag tttgatcagt gccactgcaa tgctcgccgt
     5041 tcagggcggg ttgatgggaa cgggatggac agatgtattt tcgccaaaca tttggcaggc
     5101 tgttttgcaa acgcagtttg gtggcgtctg gttatggcaa attgttctcg ccctcgtaac
     5161 gctgatcgtc gcccttatgc aaccgcggaa tatgccacgt ttgatgttta tgctcactac
     5221 cgcgcaattt attctgctgg cgggagtcgg gcatgcgacg ctgaatgaag gggtaacggc
     5281 gaaaatccat cagactaatc acgcgattca cctgatctgt gcggcggcgt ggtttggggg
     5341 attactgccg gtgctctggt gtatgcaact catcaaaggt cgttggcgac accaggctat
     5401 tcaggcgctg atgcgttttt cctggtgcgg gcattttgcg gtgatcggcg tactggcaag
     5461 cggcgtgctt aatgcattgt tgattaccgg atttcccccc acgcttacga cttactgggg
     5521 ccaactctta ttgctcaaag ccatcctggt gatgattatg gtggtcatcg cgctggctaa
     5581 tcgttatgtt cttgtaccgc gcatgaggca ggatgaagat cgtgcggcac cctggtttgt
     5641 gtggatgact aaactcgaat gggcgatagg tgcggtagtg ctggtgatca tcagcctgct
     5701 tgcgactctc gaaccttttt gatggattac gaaaatgatg aaaaaaagta ttctggcgtt
     5761 tctgttactc accagttctg cagcggcgct ggctgcaccg caggtgatta ccgttagccg
     5821 ttttgaagtg ggtaaagaca aatgggcgtt taatcgcgaa gaggtgatgc tgacttgccg
     5881 accgggtaat gctttgtatg tcatcaaccc aagtaccctc gtgcagtatc ctttaaacga
     5941 tatcgcacaa aaggaagttg ccagtgggaa gactaaagcc caacccattt cggtgattca
     6001 gattgatgat cctaacaatc ccggcgaaaa aatgagtctg gcaccgttta tagaacgagc
     6061 tgaaaaactc tgttaattac ctaaaatagc cttttgattt ccaataaaaa aaccgcctca
     6121 gttctttcac cagaacgggc ggtttttaac atttaagctg atggccaccg cgctttttat
     6181 tgaccatttt gcacgcaaac tggaaaacct ggcgtcgtca tctattctta aagggcaagg
     6241 caactaagcc tgcattaatg ccaactttta gcgcacggct ctctcccaag agccatttcc
     6301 ctggaccgaa tacaggaatc gtgttcggtc tctttttatc tgttaaaagc cagaagcatt
     6361 cccttcgctg actttatagt caaccataac acacactcta ctgtctgagt ccagcgtttt
     6421 ttaacattct tgttaagatt atgtgatctt tagcgcggga ggaaaatatt gatgaaacag
     6481 cctgcgcccg tttatcagag aattgcgggt catcaatggc gacatatctg gctttcaggc
     6541 gatatacacg gttgtcttga gcagttgcgc cgcaaattat ggcattgtgg ttttgatccg
     6601 tggcgagatt tacttatctc agtgggagac gttatcgatc gtgggccgca aagtttacgt
     6661 tgcctgcagt tactggaaca acattgggtt cgtgcggtaa gaggcaatca tgaacagatg
     6721 gcgatggatg cgctggcatc ccagcagatg tctttatggt tgatgaatgg cggcgactgg
     6781 tttattgcgc tggcagataa tcaacagaaa caagcgaaaa cggcgctgga aaaatgtcag
     6841 catttgccct ttattcttga agtacacagt cgcaccggca agcatattat tgctcatgcc
     6901 gattatccag atgatgttta tgaatggcaa aaggacgtcg atttgcatca ggtcttgtgg
     6961 agccgctcgc gattaagtga acgccaaaaa gtgcagggaa ttacaggtgc tgatcatttc
     7021 tggtttggtc atacaccgtt gcgccatcgc gtggatattg gcaacctgca ttatattgat
     7081 acaggtgttg tctttggggg cgaactgact gttgtgcaat tgcaataatt aaaaatcacc
     7141 gtactcctgt gcaggtcgcc agaaaccatc tataaaatcc tcaatcggaa aacaaccgcc
     7201 atggcggatc cgttgatcgc tcatagaata aagacactgc tgttccgtgt tgtagacatc
     7261 cacaacaata tcttcacaac cgccatccag gtagcaaaca aaaagtacca gcgcgaacat
     7321 ttcatccccg aagtgtggtg ccgtaccgtt aagtttagga gagattttac aacgggggaa
     7381 taaccaggac aaataacccg ccatgccgac gggttctttt tggattaggc aagacgcata
     7441 atccaggcat cagattgttg atcgtaacgg gtacggtaaa ggacagagtg ttcgccattt
     7501 agcagggcag ggatactggt ttcagcatcc caggcatcca gttgcataca taaatcgggg
     7561 tcagatttac aaggaatggt taacgttcga tcacccggcg attcgccgtg taattcgccg
     7621 tcgtcgattt caaaagcgcc tatgcgcaca ctggttttca tcttcgtgct ctccatttag
     7681 ctgccgattg tggtacgacc agtccgatcg caccattttt cagcctagtt gaggcagccg
     7741 aatgtgccag tcaaaaagtg cgcttttttt gtgcgctgtc aggcgttacc ggtaaaaagt
     7801 ttcccatcgc gcaccagttc acgcgggtag ctgtttttca gtcgcgaacc aattcgtttg
     7861 gctaaaccaa tcggctgatg ctggaaagta accaatacat catccgctac tggcgcggct
     7921 tgcgggtaaa catcgcgccc gcgataccac tcctccgctt cctgcgccgt tagttcaaaa
     7981 gcgttcacat tgtcgggtga ggcaagggca ataaccgctt catgctgcca gctataacct
     8041 ttgttatgcg tttcggcaag tttaatcccc aggcgagaaa atcggacttt accgatcagg
     8101 gcttcaatgc ccaccgggaa caaccacagt tctttgtcac gctgccagag gcgcaggttt
     8161 tcatcccagt ttaagccaac acctgcagcc gcctgacgaa tttgtccagc ttcgcgatct
     8221 ttcaccgggc taaacgggaa attgcccact ttgtattttg gggcgggtaa gacggggatc
     8281 gcctgagttt tacgcagacg agcaacgaag aagccttcgc agtcgtaaat ttgtgggaaa
     8341 acatgcaaaa agccttcttc ggtcagcgcc ttatttgcac cagggaagag atcgccaagc
     8401 ggtaaaaact ctactgcatc ggggtaagtc tctttaagcc accggcagac ggcttcattt
     8461 tcttcctggt ttaaggtaca ggtcgagtaa accagcgtac cgccagggcg taatgcatga
     8521 aaggcgctgt cgatcagttc ccgttgggta gctgcgattt cctgattgct ttctggtgac
     8581 cagtttttta gcgcatcggg atctttacgc accacgcctt cgccggagca gggagcgtcc
     8641 agcaaaatgg catcgaacat ttctggcact gccgcaccaa atacacggcc atcaaaatgc
     8701 gtgagcgcaa cattacggat gccacagcgg ctgatattgg catgtaacac ttttacccga
     8761 ctggcggaaa actcattggc aaggatagcc ccttcgttat tcatccgcgc ggcaatttgc
     8821 gtcgttttgg agcctggcgc agcagcgaca tccatcaccc gctgtggtgc attaccgtca
     8881 gcaaacaagg cggcaacggg caacattgaa ctggcttcct gaatataaaa caggccgctt
     8941 aaatgctcgg cggtactacc caatggcaat gcatcttcat cgtcgcgttc aatccagaaa
     9001 ccttcttcac accacggaat tggcgtaagc gtccagccat aagaggcggt taattgcagg
     9061 aaatcagcaa cggagatttt cagcgtatta acgcgaatgc tgcggcgcaa cgggcgctga
     9121 caggcggcaa gaaaatcatc aaaagagagc gtcgaaggca tcgcttcgcg catttgcgtc
     9181 agaaaggcgt ccgggaaata aacggtgtgt tgggccacga gcatatacca ctggcaaaaa
     9241 aacaggcgcg cagtttagca taaacgctcc ggcgcaggca cgccggagca gtgggcatta
     9301 tttgggaagc gcagtacccc attcacgcca ctctttcggt tcactttcct gcaacaggaa
     9361 gtgcttgcct tcctgggctt tcggtgccag tggcgtacct ggaggcgtgg cgaaggcgat
     9421 gccgccacgg ataaactggt taaaggtgcc ggttttcact acgccgcccg tcagaccaaa
     9481 gtccagactg taacccgatg ccaaccagaa gacggaattg ttacgcacca ggtgttgata
     9541 gcgtttactg atgcgcatcg caatcatcac gcgatctgac aatgtcccca gcgtcattcc
     9601 ggtaaccgta ccgacttcca gaccacggaa cagcacaggc gtaccgatgc ctaacgaacc
     9661 ggcttccggc gcttcaacaa taatgcttaa gccatccagg taacgcgaat cagttatggt
     9721 ggcctcttgt aattcaaagt cgcggcgagg attgccccgg cctggttcga cgttgatata
     9781 cggctggagg atagtatcaa gatgctcaac gccagctgcc gaaatttgcg gtgtgaccac
     9841 tgagaagcgt gtaccaccgc gagcaaaggt ctgaacatat tccggataga gcaccgcctt
     9901 tgcctgtact tcattgcgcg cggtaatcag atccagcgtc tggatttgcc cgatatcaat
     9961 accgagatag cgaattggca tcccgaccgc cagttttccg gcatcgaaag cgtgaagcgt
    10021 aatctgcccg ccaaccgcac gggccgctgt ttcggaagca tacagaatac gcttgtcgcc
    10081 tttacgctga ctggcgctgg caccgctgag gttatcgaag ctaatagccc cctttaatgc
    10141 tctggagagc ggggatgcct gtacagtcag accactacca ttcagctgaa ctttcgcccc
    10201 accttctgcc cagaacacgc tgttgctggt cagaaggttg cgatactccg gcttaatatg
    10261 cagatcgata tcaaacgcgt tagctcgcgg acgcacggta ataacttcac caacttcaaa
    10321 tttacggtac agcactaccg atcctgcctg cacatccggc agcgtctctg cactcaaact
    10381 cacggttgtg gtgggtaaat cgctaaggct gttctccagc gctttttcca gattggcata
    10441 cagtggatag ctggctttca tctcgccttt atcgcctggc agaatacgta tcccgccgtt
    10501 aatccattct gaggcgctgg caccaagaaa ctcaacgcca tccagcccca ccttcacgtc
    10561 gacacggctg ttgacgacaa atttgctatc gccttttacc agttcgcgat gctgaggctc
    10621 gatggcgacg gtaaaggtga cgcctttgct ggtgagttta cgatcaataa cctggcctac
    10681 ctgcacgccg tgaagaatga gcggctgacc agcatcaatg ccgtaacttt ctggtgctgt
    10741 cagggtcagt gtcagaacat ccggctcttg tagcagggct ttttcacctg gcacaacaac
    10801 gaactctttg cgtggctcgc catcgccggg caccaactca aaggtttttc cggtcagcag
    10861 ggcgctgaga ttggcatcgc taagggataa tttcgggttg cgtaattcga tgcgggtatt
    10921 ttcacgaagc agggtgacga cgctgggatc aacggtcatt tccccagtga ctttaccacc
    10981 aggatttaaa tccagtttag tcagctgtcc gacttccagc ccctgataca ttaacggcgt
    11041 cgagtcggcg gttaatccgg ccccactcgg cagttccagt tttattatta cgccacgctg
    11101 gctgtgggcg agatcttcat acagaccaaa ggtatcttcc gcctcggcag gtttcgactc
    11161 ttctggtgaa tcgaaggcaa tcgcaccgtt aaccagtgcc gccagacttt ccagtttcac
    11221 cttcgcgcca ctgatactga cgttggcatc aacgccggaa acgttccaga aacggctacc
    11281 ttttttcacc agatcggtaa aacgccgctc gatcaggaca tcaatcacca cgccttgctt
    11341 attgggattg atggcatagt cgtagacttt acccaccggg atcttacgga aatagaccaa
    11401 tgaaccactg ttcagtgaac cgagatcggg ggcttgcagg tggatcatca gatcgccatt
    11461 atccagccga tacttcggtt gggtatcgag tgcgacaaag tgatcctgct ctttaccttt
    11521 acctggcatc atgccgatat agttaccacc gacgagggca tccagcccgg agacacctgc
    11581 cagcgaggcc tttggcgtca ccagccagaa ctgtgtctct tcgcgcagcg catctttcat
    11641 atcagacttg atgctgacct tgacttcaat cttacgaaga tcgtcgctca ggctgatatc
    11701 ctgcacagtt ccgacttcaa cgccctgata acgaacaggg gtgcggcccg ggacaatacc
    11761 atccgccgac ataaagtcga tggtgacggt attaccccgg tcctgataac tgtcccaaat
    11821 cagccaacca gcaatcatta gcgcgatgaa cggcagcagc cagaaaggtg agatacggcg
    11881 tttattttta atctgcgctt cagtcgtcga agcgggcgtt tcctgactca tgtgcatccc
    11941 aaagtaagcg gctgtccagc cattccacag caagaatagt caaaattacc gctgcgccga
    12001 aataaaacgc agccggtccc atagtaaaag cgaggatctg atcgcgatta atcagcgaca
    12061 tggttaaaga tatgacaaac aggtcgagca tcgaccagcg accaatccag gtcaccatcc
    12121 gcagtaacag aatgcgtgtg cgtaaacctt gctggcattt aaaatgaata ctcaacagta
    12181 aagtgaacat gacgatcact ttagtaaacg gtaccagaat actggcgata aacacgattc
    12241 ccgcaacggc aatgttgcta cttgccagcg acataattcc ggaaagaatt gtatcttcct
    12301 gccgtcctcc gttcagataa atgatagaaa taggcaacag gttggcaggt aacaacaaaa
    12361 cgattgacgc taacagcgcc gcccagcatt tttgcagact atgacggcga cgcaggcgta
    12421 gcgggatatg gcaacgcggg cagcgaccac gctgatcagg atagccggta aaatggcacc
    12481 caagacagac acggagtttc tcgtccctac gcgtagcggg gcgctgcgga taaaatcgct
    12541 cccacagttc ttcgacatta agatgtgaca acgtcaccgt cgttaaaatc accaacgcca
    12601 caaaagagaa caagccgaca cccgcctgga tatgggcata atcctgtacc tttatagaag
    12661 caacgccaat gccgaccagg tagatatcca gcattaccca ctctttaagt cgttcaagca
    12721 tcagcagtac cggacgtaaa ttcattccca gtcggttacc aaaccataaa taagctatgg
    12781 aggtcaccag aatgaggggg gcaccgataa cgcagaaaaa gaccatcgac cccgttatcg
    12841 catcgccctg tttggtcatt tgccagatgc cttgcataac gttggcgtca atacgaatgc
    12901 ctaacagcca gatatgcaac agcggttcgc cccaggcaaa cggcataagc aacagcatgg
    12961 tgaaggccat tgccgccagg cgcgttagcg accagtcgcg cccgtcacga atttttgcct
    13021 gacagcgcgg acaataggca ctttgatgag aatttatctc gggcaggcta aatagcatgt
    13081 cacattgcgg gcaacgttgg taatcaccac gcggcagttc ctcaccgata gccctcactg
    13141 ttatcttttt tgtcggcgta atttgtggtg tgttaagagc catgacgtgc caaatgtaat
    13201 aagaattaac attaatctta acccatgatg ggaatgatct tgagcattaa cgcatttaat
    13261 gcttatttta ataggctggc agttcctggt aagacaacca agtgattata taatgaacaa
    13321 aacagaattt tacgcggatt taaatcgcga ctttaacgcg ctgatggcag gagaaaccag
    13381 ttttctggca acgcttgcga acaccagtgc gttgttatat gagcgtctca ctgacgtaaa
    13441 ctgggcaggt ttttatttgc ttgaggacga tacactggtg ctcggaccat ttcagggcaa
    13501 aattgcctgt gtccggatac ccgtcgggcg cggcgtgtgc ggcactgcgg ttgcccgcaa
    13561 tcaagtgcag cgtatcgagg atgttcatgc gtttgacggg catattgcct gtgatgcgac
    13621 gagtaattct gaaattgttc tgccgctggt ggtgaaaaat cagattattg gtgttctcga
    13681 catcgacagt accgtcttcg gtcgctttac agacgaggac gagcaaggct tacgtcagct
    13741 tgtggcacag cttgaaaagg tgcttgcaac gacggattac aaaaaattct ttgcgagcgt
    13801 cgcaggataa tcaacggata acgtagcatt tactgatagc gtcattataa tgacgcctgt
    13861 tcatgcctgc gcttgttggc tacgtccgtt gtaatcagga aatttcatgg aaaatcaacc
    13921 taagttgaat agcagtaaag aagtaatcgc gtttctggcc gaacgttttc cccactgttt
    13981 cagtgcggaa ggtgaagcgc gtccgctgaa aatcggtatt tttcaggatt tggtcgatcg
    14041 tgttgccggg gaaatgaacc tgagcaaaac gcaattacga tccgctttac gtctctacac
    14101 ttcgagctgg cgttatcttt acggtgttaa acccggcgca acgcgtgtcg atcttgacgg
    14161 caacccatgc ggtgagctgg acgagcaaca tgtagagcat gctcgcaagc agcttgaaga
    14221 agcgaaagcg cgtgttcagg cacagcgtgc tgaacagcaa gcgaaaaaac gcgaagctgc
    14281 cgcagctgtt ggtgagaaag aagacgcacc gcgacgcgaa cgcaagccac gtccgaccac
    14341 gccacgccgc aaagaaggcg ctgaacgtaa acctcgtgcg caaaagccgg tagagaaagc
    14401 gccaaaaaca gtaaaagcac ctcgcgaaga acagcacacc ccggtttctg acatttcagc
    14461 tctgactgtc ggacaagccc tgaaggtgaa agcgggtcaa aacgcgatgg atgccaccgt
    14521 attagaaatc accaaagacg gcgtccgcgt ccagctgaat tcgggtatgt ctttgattgt
    14581 gcgcgcagaa cacctggtgt tctgaaacgg aggccgggcc aggcatgaac atgtttttta
    14641 ggcttaccgc gttagctggc ctgcttgcaa tagcaggcca gaccttcgct gtagaagata
    14701 tcacgcgtgc tgatcaaatt ccggtattaa aggaagagac gcagcatgcg acggtgagtg
    14761 agcgcgtaac gtcgcgcttc acccgttctc attatcgcca gttcgacctc gatcaggcat
    14821 tttcggccaa aatctttgac cgctatctga atctgctcga ttacagccac aacgtgctgc
    14881 tggcaagcga tgttgaacag ttcgcgaaaa agaaaaccga gttaggcgat gaactgcgtt
    14941 caggcaaact cgacgttttc tacgatctct acaatctggc gcaaaagcgt cgttttgaac
    15001 gttaccagta cgctttgtcg gtactggaaa agccgatgga tttcaccggt aacgacactt
    15061 ataaccttga ccgcagcaaa gcgccctggc cgaaaaacga ggctgagttg aacgcgctgt
    15121 gggacagtaa agtcaaattc gacgagttaa gcctgaagct ggcaggaaaa acggataaag
    15181 aaattcgtga aaccctgact cgccgctaca aatttgccat tcgtcgtctg gcgcaaacca
    15241 acagcgaaga tgttttctcg ctggcaatga cggcgtttgc gcgtgaaatc gacccgcata
    15301 ccaactatct ttccccgcgt aataccgaac agttcaacac tgaaatgagt ttgtcgctgg
    15361 aaggtattgg cgcagttctg caaatggatg atgactacac cgttatcaat tcgatggtgg
    15421 caggtggtcc ggcagcgaag agtaaagcta tcagcgttgg tgacaaaatt gtcggtgttg
    15481 gtcaaacagg caagccgatg gttgacgtga ttggctggcg tcttgatgat gtggttgcct
    15541 taattaaagg gccgaagggc agtaaagttc gtctggaaat tttacctgct ggtaaaggga
    15601 ccaagacccg tactgtaacg ttgacccgtg aacgtattcg cctcgaagac cgtgcggtta
    15661 aaatgtcggt gaagaccgtc ggtaaagaga aagtcggcgt tctggatatt ccaggtttct
    15721 atgtgggttt gacagacgat gtcaaagtgc agttgcagaa actggaaaaa cagaatgtca
    15781 gcagtgtcat catcgacctg cgtagcaaag gcggtggggc gttgaccgaa gcggtatcgc
    15841 tctccggtct gttcattcca tccggtccga ttgttcaggt ccgtgataac aacggtaaag
    15901 ttcgtgaaga cagcgatacc gatgggcagg ttttctataa aggcccgctg gtggtgctgg
    15961 ttgaccgctt cagtgcttcg gcttcagaaa tctttgccgc ggcaatgcag gattacggtc
    16021 gtgcgctggt tgtgggtgaa ccgacgtttg gtaaaggcac cgttcagcaa taccgttcat
    16081 tgaaccgtat ttacgatcag atgttacgtc ctgaatggcc agcgctgggt tctgtgcagt
    16141 acacgatcca gaaattctat cgcgttaacg gcggcagtac gcaacgtaaa ggcgtaacgc
    16201 cagacatcat catgccgacg ggtaatgaag aaacggaaac tggtgagaaa ttcgaagata
    16261 acgcgctgcc gtgggatagc atcgatgccg cgacctatgt gaaatcaggc gatttaacgg
    16321 cctttggacc ggagctgctg aaggaacata atgcgcgtat cgcgaaagat cctgagttcc
    16381 agaacatcat gaaggatatc gcgcgcttca acgccatgaa ggacaagcgc aatatcgttt
    16441 ctctgaatta cgctgtgcgt gagaaagaga ataatgaaga tgatgcgacg cgtctggcgc
    16501 gtttgaacga acgttttaaa cgcgaaggta aaccggagtt gaagaaactg gatgatctac
    16561 cgaaagatta ccaggagccg gatccttatc tggatgagac ggtgaatatc gcactcgatc
    16621 tggcgaagct tgaaaaagcc agacccgcgg aacaacccgc tcccgtcaag taatatcaat
    16681 caggcacaag aaattgtgcc tgatttttta acagcggcaa gatgccgtaa atcagatgct
    16741 acaaaatgta aagttgtgtc tttctggtga cttacgcact atccagactt gaaaatagtc
    16801 gcgtaaccca tacgatgtgg gtatcgcata ttgcgttttg ttaaactgag gtaaaaagaa
    16861 aattatgatg cgaatcgcgc tcttcctgct aacgaacctg gccgtaatgg tcgttttcgg
    16921 gctggtactg agcctgacag ggatacagtc gagcagcgtt caggggctga tgatcatggc
    16981 cttgctgttc ggttttggtg gttccttcgt ttcgcttctg atgtccaaat ggatggcatt
    17041 acgatctgtt ggcggggaag tgatcgagca accgcgtaac gaaagggaac gttggctggt
    17101 caatactgta gcaacccagg ctcgtcaggc ggggatcgct atgccgcaag tggctatcta
    17161 ccatgcgccg gacatcaacg cttttgcaac cggtgcgcgc cgtgatgcct ctctggttgc
    17221 tgtcagcacc ggtttgctgc agaacatgag cccggatgaa gccgaggcgg taattgctca
    17281 cgaaatcagc cacatcgcca atggtgatat ggtcaccatg acgctgattc agggcgtggt
    17341 gaacaccttc gttatcttta tttcccgtat tctggcgcag cttgccgcgg gttttatggg
    17401 cggaaatcgt gatgaaggtg aagagagcaa cggcaacccg ctgatctact ttgcggttgc
    17461 aacggttctg gaactggtgt ttggtattct ggcgagcatt atcaccatgt ggttctcgcg
    17521 tcatcgtgaa ttccatgctg atgccggttc ggcaaaactg gttggtcgcg agaaaatgat
    17581 tgccgcgctg cagcgcctga aaaccagcta tgaaccgcaa gaagcaacca gcatgatggc
    17641 tttctgtatt aacggtaagt cgaaatcgct cagtgagttg ttcatgactc acccgccgct
    17701 ggataaacgt attgaagctc tgcgtacggg tgaatacctg aagtaatatg aagtaaatac
    17761 taaaaagcgc gtcctgatcg acgcgctttt tttatgccct ggctcgtggc tgagtgatac
    17821 gtagaccact gacacaggca gcaattactg ccagaatagc cgcagccatc agcgagacgt
    17881 gcgtaccatt atcaccaaac tgatttagca tcagcgccac cagcgccgcg ccactactct
    17941 gacccaatag acgagccgtc cctaacatgc cactggcccc accgctacgt tcgcgcggag
    18001 cggaggtaat aatggtgtgg ttattgggtg actgaaataa gccaaatcca gcgccacata
    18061 agatcatcgg ccagataata ttgatatccg caggtgacgc gggcagcaga accagggaaa
    18121 aaagccccgc agccatgatg aacaacccta aagctcccag caatcctgca tgtacgcgtt
    18181 caatcaaata gcctgccagc ggagccatca ccattgttgc taacggccac ggtgtcagaa
    18241 gtaaacctgt ttcgacttca ctacgcccga gcacggtttg caggtaaaag ggcagggaaa
    18301 ccattgccag catttgtgcg cagaaagagc aaacagatgt gcaaatagaa agtgaaaaca
    18361 gcgggatacg cagtaaatcc accggtagca gcggtacggg aagagaaagc tggcggcgaa
    18421 taaagaaaat accaacgaca accattacca ccagttccgc gccaatcaac gtcagcgatt
    18481 gcccctgagc gaaaccactc aatgcagtaa taagcaggcc gaaggttaac gcgttcatca
    18541 cggcgctggg caggtcgaaa cggggtttac tggcacgaga gccattgggt ggcagaaaac
    18601 gcatcgccag aagcagggcg ataataccca acggtacgtt gattaaaaat aaccatttcc
    18661 aggatgcgat ggagaggatt gctgcagcaa ttgtcggacc ggcagcagaa gatacggcaa
    18721 caataaacga gtttatgccc atccctctac ccagaaaacg ttgtggatag atcaggcgga
    18781 taagtgcggt attaacgctc atcaacgctg caccgccgaa accttgtatg acacgcgcaa
    18841 gggtgagcat ttgcagcgaa tcagaaaggg cgcagaacag tgaagacaac agaaaaacga
    18901 ccagaccgca tttataaata cgtcgatagc caaacatatc gcccagaaac gaaaacgaga
    18961 gcagggagat gacaatggcg atttgatagg cgttcactac ccagatggaa ctggctggcg
    19021 tggcatgaag gtccgtggcg attgttggca gggcgacgtt ggcgattgcg ccgtcaagga
    19081 cagccatcga aataccaatc acaatggtta atatcgcacc gtatcgctgg ggcaatggca
    19141 ggccgtcggc ctgaactttt ggcataggaa ttttatatct ttggtgaata atcaacaggg
    19201 gtatgattct agcactgata ttcacggtgg atgtcgcaga attgtaacta accgacaaaa
    19261 gatggattgc gaccaccctg gcgcgaaatt ataataaaaa acagttctga tttttataaa
    19321 acactcgcaa tgaggtgata aatggctaac gcagatctgg ataaacagcc tgattctgta
    19381 tcttccgtgc tgaaagtttt tggcattttg caggcgctgg gtgaagagcg cgaaataggg
    19441 ataaccgagc tgtcgcagcg cgtcatgatg tcaaaaagca ccgtttatcg ctttttacag
    19501 accatgaaaa ccttaggtta tgtggcgcag gaaggggagt cggagaaata ttccctgacc
    19561 ctgaaattgt ttgaactggg cgctcgcgcg ttacaaaacg tcgatttaat tcgtagcgca
    19621 gatatccaga tgcgtgagat ctcccgcctg accaaagaaa ctatccacct cggcgcactg
    19681 gacgaagaca gtattgttta cattcacaaa attgactcta tgtacaattt gcgcatgtat
    19741 tcacggattg ggcgtcgtaa tccgctgtac agcaccgcga ttggtaaggt actgctggca
    19801 tggcgcgatc gcgatgaagt gaagcaaatt cttgagggcg tggagtataa acgcagtacc
    19861 gagcggacca tcaccagtac agaagcgtta ttacccgttc tggaccaggt gcgtgagcag
    19921 gggtatggcg aagataacga agagcaggaa gaagggctgc gatgcattgc ggttccggta
    19981 tttgatcgct ttggcgtggt cattgccggt cttagcatct ccttcccgac gttgcgtttc
    20041 tctgaagagc gtttacagga atatgtcgca atgttgcata ccgcggcgcg caaaatttct
    20101 gcccaaatgg gttatcacga ctatccgttc tgatgagagt aagaacctgt ctgaatatca
    20161 aacagacagg ttctttattt agcatgagaa aaataaagtt gaaggtggcg ttatattaaa
    20221 cgcacttgcg ataagaatat tttactcatg agtgagaatc tggttattta ttacccttaa
    20281 ccattatcga ccacaatatt gcttttacgt aacaacgggc aatctgttat ccccagaaaa
    20341 ccacttttag tgtgcaggta ttgtaccgtg ctggtgcctc tggcggtcag ataggtacat
    20401 tgcaaaccta atcccgcggc attctctttg cttccaatca aaacgccata tccgctgagt
    20461 aataatccta tccataccag tgctatcagc ataactgtgc gaatgatgaa tcgcattaca
    20521 acctcttctc tttttatgtt cgcttaatcg tagcggcaat atgcgctgaa gcaagcgact
    20581 cattctgaaa aagcacgaat atcgacatag ttaggcgctg tttaactaac gcatgctagt
    20641 ttaatgacat aaggtaggtg aaacggagat tggagtgaaa aagtttcgat gggtcgttct
    20701 ggttgtcgtg gtgttggctt gcttgctgct ttgggcgcag gtattcaaca tgatgtgcga
    20761 tcaggatgta caatttttca gcggaatttg tgccattaac cagtttatcc cgtggtgata
    20821 tcattttttc agcactagat tttcttcctc tccagcagtg gtagaatagc gcctctactc
    20881 agaggtggtg aaatgaacga agttgtaaat tcaggcgtga tgaacattgc gtctttggtt
    20941 gtatcggtgg tggttctttt tatcgggctt atcttgtggt tttttatcaa tcgtgccagt
    21001 tcacggacta acgaacagat tgaactgctt gaggcgttgc tggatcagca aaaacgtcaa
    21061 aatgctctgt tacgtcgttt gtgcgaagca aacgaacctg agaaagcaga taaaaagacc
    21121 gttgagagtc aaaaatcggt tgaagacgaa gatattattc gtctggtcgc cgaacgataa
    21181 tccttcctca atcttgagta accccgtcgt tgaagggtta ctcaattcat cctgattata
    21241 cgcactgtca tggtgcaact gcttcaaatt aaaactctgg tctgacttca aataattatc
    21301 atattattta tttgcgtaac gggata
//
LOCUS       DACBVI010000071        21222 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.071, whole genome shotgun sequence.
ACCESSION   DACBVI010000071 DACBVI010000000
VERSION     DACBVI010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.071"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(401..715)
                     /gene="fliE"
                     /locus_tag="IU325_12930"
     CDS             complement(401..715)
                     /gene="fliE"
                     /locus_tag="IU325_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HAP5809925.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTFGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            930..2588
                     /gene="fliF"
                     /locus_tag="IU325_12935"
     CDS             930..2588
                     /gene="fliF"
                     /locus_tag="IU325_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body M-ring protein FliF"
                     /protein_id="HAP5809926.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIATIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            2581..3576
                     /gene="fliG"
                     /locus_tag="IU325_12940"
     CDS             2581..3576
                     /gene="fliG"
                     /locus_tag="IU325_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HAP5809927.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            3569..4255
                     /gene="fliH"
                     /locus_tag="IU325_12945"
     CDS             3569..4255
                     /gene="fliH"
                     /locus_tag="IU325_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HAP5809928.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLARGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            4255..5628
                     /gene="fliI"
                     /locus_tag="IU325_12950"
     CDS             4255..5628
                     /gene="fliI"
                     /locus_tag="IU325_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HAP5809929.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGIGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            5647..6090
                     /gene="fliJ"
                     /locus_tag="IU325_12955"
     CDS             5647..6090
                     /gene="fliJ"
                     /locus_tag="IU325_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HAP5809930.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            6087..7214
                     /gene="fliK"
                     /locus_tag="IU325_12960"
     CDS             6087..7214
                     /gene="fliK"
                     /locus_tag="IU325_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HAP5809931.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVAGNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            7319..7783
                     /gene="fliL"
                     /locus_tag="IU325_12965"
     CDS             7319..7783
                     /gene="fliL"
                     /locus_tag="IU325_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HAP5809932.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            7788..8792
                     /gene="fliM"
                     /locus_tag="IU325_12970"
     CDS             7788..8792
                     /gene="fliM"
                     /locus_tag="IU325_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HAP5809933.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            8789..9202
                     /gene="fliN"
                     /locus_tag="IU325_12975"
     CDS             8789..9202
                     /gene="fliN"
                     /locus_tag="IU325_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HAP5809934.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            9205..9570
                     /gene="fliO"
                     /locus_tag="IU325_12980"
     CDS             9205..9570
                     /gene="fliO"
                     /locus_tag="IU325_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HAP5809935.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     VPKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQPDFQSVMKNLLKRSGRS"
     gene            9570..10307
                     /gene="fliP"
                     /locus_tag="IU325_12985"
     CDS             9570..10307
                     /gene="fliP"
                     /locus_tag="IU325_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HAP5809936.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            10317..10586
                     /gene="fliQ"
                     /locus_tag="IU325_12990"
     CDS             10317..10586
                     /gene="fliQ"
                     /locus_tag="IU325_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HAP5809937.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            10595..11380
                     /gene="fliR"
                     /locus_tag="IU325_12995"
     CDS             10595..11380
                     /gene="fliR"
                     /locus_tag="IU325_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HAP5809938.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            11670..12293
                     /gene="rcsA"
                     /locus_tag="IU325_13000"
     CDS             11670..12293
                     /gene="rcsA"
                     /locus_tag="IU325_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HAP5809939.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(12337..12579)
                     /gene="dsrB"
                     /locus_tag="IU325_13005"
     CDS             complement(12337..12579)
                     /gene="dsrB"
                     /locus_tag="IU325_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HAP5809940.1"
                     /translation="MPEKRNCSTLAQSHRRKTMKVNDRVTVKTDGGPRRPGVVLAVEE
                     FSEGTMYLVSLEDYPLGIWFFNEAGHQDGIFVEKAE"
     gene            12688..12915
                     /gene="yodD"
                     /locus_tag="IU325_13010"
     CDS             12688..12915
                     /gene="yodD"
                     /locus_tag="IU325_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HAP5809941.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            13213..14028
                     /locus_tag="IU325_13015"
     CDS             13213..14028
                     /locus_tag="IU325_13015"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HAP5809942.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(14025..15719)
                     /gene="yedQ"
                     /locus_tag="IU325_13020"
     CDS             complement(14025..15719)
                     /gene="yedQ"
                     /locus_tag="IU325_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HAP5809943.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFVGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSENYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRLLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNSGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(15890..16072)
                     /locus_tag="IU325_13025"
     CDS             complement(15890..16072)
                     /locus_tag="IU325_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HAP5809944.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(16151..17068)
                     /locus_tag="IU325_13030"
     CDS             complement(16151..17068)
                     /locus_tag="IU325_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HAP5809945.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            17241..18161
                     /gene="yedA"
                     /locus_tag="IU325_13035"
     CDS             17241..18161
                     /gene="yedA"
                     /locus_tag="IU325_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HAP5809946.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(18150..18620)
                     /gene="vsr"
                     /locus_tag="IU325_13040"
     CDS             complement(18150..18620)
                     /gene="vsr"
                     /locus_tag="IU325_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch endonuclease Vsr"
                     /protein_id="HAP5809947.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGLGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGCRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(18601..20019)
                     /gene="dcm"
                     /locus_tag="IU325_13045"
     CDS             complement(18601..20019)
                     /gene="dcm"
                     /locus_tag="IU325_13045"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP5809948.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKDGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AIFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(20086..20781)
                     /locus_tag="IU325_13050"
     CDS             complement(20086..20781)
                     /locus_tag="IU325_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP5809949.1"
                     /translation="MDLQHWQTQFENWLKNHHQHQDAAHDVYHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(20821..21178)
                     /locus_tag="IU325_13055"
                     /pseudo
     CDS             complement(20821..21178)
                     /locus_tag="IU325_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
BASE COUNT         5133 a         5641 c         5462 g         4986 t
ORIGIN      
        1 tatttaattg aaccgactga gtttttaacg aaagatcatt taatggaaac atttacactc
       61 ctgtttaata aattatattg cccagggcgc attctaaaac acaataaaat taccactatc
      121 aaaaatcgct catatccata gcccggaata tgaataaaaa catcaacaaa gattaaatta
      181 tccagaggtt actgttgcgt ctgtggtcgt aacaacatcc gacgtggtga tgtgtcaaat
      241 gagacgctct gatgttcagg ggggttatcg cgtatacgga tcgtggatgg cagaaaacgt
      301 tcaggatcag gtatccattt taaaccagaa aatgagtgag tttgccccat ccatgcctca
      361 cgcggtgaga tcggatgtaa tcaacaacag gttacaaaac ctacacctgc atgctcatca
      421 cttcctgata cgccgccacc agcttattac gcacctgaat ccccatttgc atagaaactg
      481 aggctttttg catatcggtc atcacatcgt ttaacgccac gccgggttca ccgaaagtga
      541 atttttctgc ctgcgtgcgg gcggctgttt gtgtatcgct tatgcgatcg agcgcggcgt
      601 gcagctgccc ggcaaaacta atggtcggtt gcggcagtga ttcctgcgca cgcgcactca
      661 tcgccgtagc ctgtaactgg ctgataaccc cttcaatccc ctgtatcgct gacattttca
      721 tctcctggat aatttctggt agcaaagcct accagtaagt caataagaca aaggcgctaa
      781 atagcaacaa aaaaacgggt ttattggcgg atagaaaaaa acgaaagcac aaataatggc
      841 agcgtcaatt tttcgagttt gctgacccgg gagtgagtct tgttccactt tgccaataac
      901 gccgtccata atcagccacg aggtgcgcga tgaatgcgac tgcagcccag acaaaatctc
      961 ttgagtggct taatcgcctg cgtgcgaatc cgaaaattcc attgattgtt gccggttccg
     1021 cggcagtggc ggtcatggtc gcactgatcc tgtgggcgaa agcccccgac taccgcacat
     1081 tattcagcaa tctttccgat caggatggtg gcgcaattgt cagccaactg acgcaaatga
     1141 atattcctta ccgcttcagc gaagccagcg gcgctattga agttccggca gataaagttc
     1201 acgaactgcg tctgcgcctg gcacaacaag gtttgcccaa aggcggcgcg gtcggtttcg
     1261 aactactcga tcaggaaaag tttggtatca gccagttcag cgaacaggtg aattatcagc
     1321 gggcgctgga aggcgagctt tctcgtacca tcgcaactat cggcccggta aaaggggcgc
     1381 gcgtacatct ggcaatgccg aaaccgtctt tattcgtccg tgaacaaaaa tccccttctg
     1441 catcggtgac ggtaaatctg ttacccggcc gcgcactcga tgaagggcaa attagcgcca
     1501 ttgtgcatct ggtttccagc gccgttgctg gtctgccgcc gggaaacgtc acgctggtgg
     1561 atcagggcgg acatctgtta acccagtcca ataccagcgg gcgcgatctt aatgatgctc
     1621 agttgaaata tgccagcgat gtcgaaggcc gtattcagcg gcgtattgaa gcaatcctgt
     1681 cgcctattgt tggtaacggt aatattcacg cccaggtcac ggcgcagctg gacttcgcca
     1741 gtaaagaaca aacggaagaa cagtatcgcc ctaacggtga tgaatctcat gcggcgcttc
     1801 gttcacgcca gcttaatgag agcgagcaaa gcggttccgg ttatccgggc ggcgtaccgg
     1861 gggcgttgtc gaatcaaccg gcacctgcga ataacgcgcc aatcagcacg cctccggcaa
     1921 atcaaaataa ccgccagcag caggcgagca ccaccagcaa tagcgggccg cgtagcacac
     1981 agcggaatga aaccagtaac tacgaagtcg atcgcaccat tcgtcatacc aaaatgaacg
     2041 tgggcgatgt gcaacgtctg tcagtcgcgg tagtggtgaa ttacaaaacc ttgccagacg
     2101 gtaaaccatt gcctctcagc aacgaacaga tgaagcaaat tgaagatctg acccgcgagg
     2161 cgatgggctt ttctgaaaaa cgcggtgact cgctcaatgt cgttaactcg ccgttcaata
     2221 gcagtgacga aagcggcgga gaactgccgt tctggcaaca gcaagcgttt atcgatcagt
     2281 tgctggctgc cggtcgctgg ttgctggtac tgctggtggc gtggctgctg tggcggaaag
     2341 cggtacgccc gcagttaaca cgtcgcgccg aggcgatgaa agctgtacag caacaggcgc
     2401 aggcccgtga ggaagtggaa gatgcggtgg aagtccgcct gagcaaagac gaacaactcc
     2461 aacaacgacg cgctaaccaa cgtctggggg cagaagtcat gagccagcgt atccgtgaaa
     2521 tgtctgataa cgatccgcgc gtggtggcgc tggtcattcg ccagtggata aataacgatc
     2581 atgagtaacc tgacaggcac cgataaaagc gtcatcctgc tgatgaccat tggcgaagac
     2641 cgggcggcag aggtgttcaa gcacctctcc cagcgcgaag tgcaaaccct gagcgctgca
     2701 atggcgaacg tcacgcagat ctccaacaag cagctaaccg atgtgctggc ggagtttgag
     2761 caagaagctg aacagtttgc cgcactgaat atcaacgcca acgattatct gcgttcggta
     2821 ttggtcaaag ctctgggtga ggaacgtgcc gccagcctgc tggaagatat tctcgaaact
     2881 cgcgataccg ccagcggtat tgaaacgctc aactttatgg agccgcagag tgccgccgat
     2941 ctgattcgcg atgagcatcc gcaaattatc gccaccattc tggtgcatct gaagcgcgcc
     3001 caggccgccg atattctggc gttgttcgat gaacgtctgc gccacgacgt gatgttgcgt
     3061 atcgccacct ttggcggcgt gcagccagcc gcgctggcgg aactgaccga agtactgaat
     3121 ggcttgctcg acggtcagaa tctcaagcgc agcaaaatgg gcggcgtgag aacggcagcc
     3181 gaaattatca acctgatgaa aactcagcag gaagaagccg ttattaccgc cgtgcgcgaa
     3241 ttcgacggcg agctggcgca aaaaattatc gacgagatgt tcctgttcga aaatctggtg
     3301 gatgtcgacg atcgcagcat ccagcgtctg ttgcaggaag tggattccga atcgctgttg
     3361 atcgcgctga aaggggccga gcagccactg cgcgagaaat tcctgcgcaa tatgtcgcag
     3421 cgtgccgccg atattctgcg cgacgatctc gccaaccgtg gtccggtgcg tctgtcgcag
     3481 gtggaaaacg aacagaaagc gattctgctg attgtgcgcc gccttgccga aactggcgag
     3541 atggtaattg gcagcggcga ggatacctat gtctgataat ctgccgtgga aaacctggac
     3601 gccggacgat ctcgcgccac cacaggcaga gtttgtgccc atggtcgagc cggaagaaac
     3661 catcattgaa gaggccgaac ccagccttga gcagcaactg gcgcaactgc aaatgcaggc
     3721 ccatgagcaa ggttatcagg cggggattgc cgaaggtcgc cagcaaggtc atgagcaggg
     3781 ctatcaggaa ggactggccc gggggctgga gcaaggtctg gcagaggcga agtctcaaca
     3841 agcgccaatt catgcccgga tgcagcaact ggtcagcgaa tttcaaacta cccttgatgc
     3901 gcttgatagt gtgattgctt cgcgcctgat gcagatggcg ctggaggcgg cacgtcaggt
     3961 catcggtcag acgccaacag tggataactc ggcactgatc aaacagatcc aacagttgtt
     4021 gcagcaagaa ccgttattca gcggtaaacc acagctgcgc gtgcacccgg atgatctgca
     4081 acgtgtggat gatatgctcg gcgcaacctt aagtttgcat ggctggcgct tgcggggcga
     4141 tcccacactc catcctggcg gctgtaaagt ctccgccgat gaaggcgatc tcgacgccag
     4201 tgtcgccact cgctggcaag aactctgccg tctggcagca ccaggagtgg tgtaatgacc
     4261 acgcgcctga ctcgctggct aaccacgctg gataactttg aagccaaaat ggcgcagttg
     4321 cctgcggtac gtcgctatgg gcgattaacc cgcgctaccg ggctggtgct ggaagccacc
     4381 ggattacaat tgccgctcgg cgcaacctgt gtcattgaac gccagaacgg cagcgaaacg
     4441 cacgaagtag aaagcgaagt cgttggcttt aacggtcaac ggctgttttt aatgccgctg
     4501 gaggaagtcg aaggtgtcct gcccggcgcg cgggtttatg ccaaaaacat ttcggctgaa
     4561 ggactgcaaa gcggcaagca gttgccgctc ggtccggcgt tattaggtcg cgttctggac
     4621 ggcagcggta agccgctcga tggcctgccc tcccccgata cgacggaaac cggtgcgctg
     4681 attaccccgc catttaaccc gttgcaacgt acaccgattg aacatgtgct ggacaccggc
     4741 gtgcgcccaa tcaatgccct gcttaccgtt gggcgtgggc agcgtatggg actgtttgcc
     4801 gggtccggca ttggtaaaag tgtgctgctg gggatgatgg cacgttacac ccgcgccgat
     4861 gtcattgtcg tgggtttgat tggtgaacgc ggacgcgaag taaaagattt tattgagaat
     4921 atcctcggtg ccgaagggcg tgcacgctcc gtggtgattg ccgctccggc ggatgtttct
     4981 cctctcctgc gaatgcaggg tgccgcctat gccacgcgca ttgccgaaga ttttcgcgat
     5041 cgtggtcagc atgtgttgct gattatggac tccctcaccc gctacgcgat ggcccagcgt
     5101 gaaattgcgc tggcgattgg cgaaccaccc gccactaaag gttatccccc gtcggtgttt
     5161 gccaaattac cggcactggt cgagcgtgcc ggaaatggca ttagcggcgg cggatcgatt
     5221 actgcatttt ataccgtgct cactgaaggc gatgaccagc aggaccccat tgccgactct
     5281 gcgcgggcaa tcctcgacgg tcacattgtg ctgtctcgcc gactggcgga agccgggcac
     5341 tatccggcta tcgatattga agcgtcgatc agccgcgcaa tgacggcgtt gatcagtgag
     5401 caacattacg cgcgagtgcg caccttcaaa cagctgttgt cgagttttca gcgtaaccgc
     5461 gatctggtta gcgtcggcgc gtatgccaaa ggcagcgatc cgatgctcga caaagccatc
     5521 gccctgtggc cgcagctgga gggctatttg caacaaggca tttttgaacg cgcggactgg
     5581 gaagcgtctc tccaggggct ggagcgtatt ttcccgacag tgtcataacc caggagataa
     5641 cggcagatgg cagaacatgg cgcgctggcg accctgaaag atctggcaga aaaagaggta
     5701 gaggatgccg cgcgcctgct gggtgaaatg cgtcgcgggt gtcagcaggc ggaagaacag
     5761 ctcaaaatgc tgatcgatta tcagaatgaa tatcgcaata acctcaacag cgatatgagt
     5821 gccgggatga ccagcaaccg ctggatcaac tatcagcagt ttatccagac gctggaaaaa
     5881 gccattactc agcatcgcca gcaacttaat cagtggacgc agaaagttga cattgccctg
     5941 aacagctggc gagaaaagaa acaacgtttg caggcctggc agacactgca ggaacggcaa
     6001 tccacggcgg cactgcttgc agaaaaccgc ctcgatcaga aaaagatgga tgagttcgcc
     6061 cagcgcgccg ccatgaggaa acctgaatga ttcgcttagc gcccttaatt accgccgacg
     6121 ttgacaccac cacattgcct ggcggcaaag ccagcgatgc tgcacaagat tttctcgcgt
     6181 tgttgagcga agcattagca ggcgagacaa ctaccgacaa agcggccccc cagttgctgg
     6241 tggcaacaga taagcccacg acaaaaggcg agccgctggt cagcgagatt cttgccgatg
     6301 cgcaacaagc ggatttactg atccctgtgg atgaaacacc gcctgtcatc aacgacgaac
     6361 aatccacatc aacaccatta accaccgctc aaacgatgac gttggctgcg gtggctggca
     6421 acaatacggc aaaagacgaa aaagcggatg atctgaatga agacgtcacc gcaagcctga
     6481 gcgccctttt tgcgatgttg ccgggttttg acaatacgcc caaagtgact gatgtgccgt
     6541 caaccgtgtt accggcagag aaaccaacgc tcttcacaaa actgacttct gcgcaactca
     6601 caacagcaca gcctgatgat gcccccggca cgccagctca gccattaaca ccgctggtag
     6661 cagaagccca gagtaaagcg gaagtcatca gcacaccttc accggtgacc gctgccgcca
     6721 gcccgctaat tactccacac cagacacagc cactgcccac cgtcgccgcg cctgttttga
     6781 gtgcaccgct gggttctcac gaatggcaac aatcattaag ccagcatatt tcgctgttca
     6841 cccgccaggg gcaacaaagt gcagagttgc gtctgcaccc gcaggattta ggtgaagtgc
     6901 aaatctccct caaagtggat gataaccagg cgcaaatcca gatggtttca ccgcatcagc
     6961 atgtacgcgc cgccctggaa gcagcgctgc cggtactgcg tacgcagctg gccgaaagtg
     7021 gcattcagtt agggcaaagc aacatcagtg gcgaaagctt tagtggtcag cagcaggccg
     7081 cttcccaaca acagcaaagc caacgcacag taaaccatga acctctggcg ggggaagaag
     7141 acgatacgct tccggttccc gtctctttac aagggcgcgt aacaggcaac agcggcgttg
     7201 atattttcgc ctaacgtcag aggtagcacc gtaatccgcg tcttttcccc gctttgttgc
     7261 gctcaagacg caggataatt agccgataag cagtagcgac acaggaagac cgcaacatat
     7321 gactgattac gcgataagca agaaaagcaa gcgatcgctt tggatcccga ttctggtatt
     7381 cattaccctc gcggcctgtg ccagcgcagg ttacagctac tggcattcgc atcaggttgc
     7441 cgctgacgac aaagcgcagc aacgtgtcgt gccctcaccg gtcttctatg cgctggatac
     7501 cttcacggtc aatttgggcg atgcggatcg cgtgctttat atcggcataa ccctgcgcct
     7561 gaaagatgaa gctacccgct cgcggctgag tgagtatttg ccggaagtcc gtagccgttt
     7621 gctgttactg ttttcgcgtc aggatgctgc cgtactggcg acagaagaag gcaagaaaaa
     7681 cctgattgcc gagattaaaa ccacactttc caccccgctt gttgccgggc aaccgaaaca
     7741 ggatgtcacc gacgtgctgt ataccgcttt tattctgcga taacgacatg ggcgatagta
     7801 ttctttctca agctgaaatt gatgcgctgt tgaatggtga cagcgaagtc aaagacgaac
     7861 cgacagccag tgttagcggc gaaagtgaca ttcgtccgta cgatccgaat acccaacgac
     7921 gggttgtgcg cgaacgtttg caggcgctgg aaatcattaa tgagcgcttt gcccgccatt
     7981 ttcgtatggg gctgttcaac ctgctgcgtc gtagcccgga tataaccgtc ggggccatcc
     8041 gcattcagcc gtaccatgaa tttgcccgca acctgccggt gccgaccaac ctgaacctta
     8101 tccatctgaa accgctgcgc ggcacagggc tggtggtgtt ttcaccgagt ctggtgttta
     8161 tcgccgtgga taacctgttt ggcggcgatg gacgcttccc gaccaaagtg gaaggtcgcg
     8221 agtttaccca taccgaacag cgcgtcatca accgcatgtt aaagctggcg cttgaaggct
     8281 atagcgacgc ctggaaggcg attaatccgc tggaagttga gtacgtgcgt tcggaaatgc
     8341 aggtgaaatt taccaatatc accacctcgc cgaacgacat tgtggttaac acgccgttcc
     8401 atgtggagat tggcaacctg accggcgaat ttaatatctg tctgccattc agcatgattg
     8461 agccgctacg ggaattgttg gttaacccgc cgctggaaaa ctcgcgtaat gaagatcaga
     8521 actggcgcga taacctggtg cgccaggtgc agcattcaca gctggagttg gtcgccaact
     8581 ttgccgatat ctcgctacgc ctgtcgcaga ttttaaaact gaaacccggc gacgtcctgc
     8641 cgatagaaaa acccgatcgc atcatcgctc atgttgacgg cgttccggta ctgaccagtc
     8701 agtacggcac cctcaacggt cagtatgcgt tacggataga acatttgatt aacccgattt
     8761 taaattctct gaacgaggaa cagcccaaat gagtgacatg aataatccgg ccgatgacaa
     8821 caacggcgca atggacgatc tgtgggctga agctttgagc gaacaaaaat caaccagcag
     8881 caaaagcgct gccgacgcgg tgttccagca atttggcggt ggtgatgtca gcggaacgtt
     8941 gcaggatatc gacctgatta tggatattcc ggtcaagctg accgtcgagc tgggccgtac
     9001 gcggatgacc atcaaagagc tgttgcgtct gacgcaaggg tccgtcgtgg cgctggacgg
     9061 tctggcgggc gaaccactgg atattctgat caacggttat ttaattgccc agggcgaagt
     9121 ggtggtcgtt gccgataaat atggcgtgcg gatcaccgat atcattactc cgtctgagcg
     9181 aatgcgccgc ctgagccgtt agtgatgaat aaccacgcta ctgtgcaatc ttccgcgccg
     9241 gtttctgctg cgccactgct gcaggtgagc ggcgcactca tcgccattat tgccctgatc
     9301 ctcgctgctg cctggctggt aaaacggttg ggatttgtcc ctaaacgcac tggcgttaac
     9361 ggtctgaaaa ttagcgccag tgcttcgctg ggcgcgcgtg aacgggttgt ggtggtcgat
     9421 gtggaagatg cacggctggt gctcggcgtt accgcagggc aaatcaatct gctgcataaa
     9481 cttccccctt cagcaccaac ggaagagata ccgcagcccg attttcagtc ggtcatgaaa
     9541 aacttgctta agcgtagcgg gagatcctga tgcgtcgttt gttgtctgtc gcccctgtcc
     9601 ttctctggct gattacgccc ctcgccttcg cgcaactgcc gggtatcacc agccagccgc
     9661 tgcctggcgg tggacaaagc tggtcgctcc cggtgcagac gctggtgttc atcacctcgt
     9721 tgacgtttat tccggcaatt ttactgatga tgaccagttt cacccgcatc attattgttt
     9781 ttggtttatt gcgtaacgcg ctgggaacac cctccgcgcc acctaaccag gtattgctgg
     9841 ggttggcgct gtttttgacc ttttttatta tgtcgccggt gatcgacaaa atttatgtcg
     9901 atgcgtacca gccattcagc gaagagaaaa tatcaatgca ggaggcgctg gaaaaagggg
     9961 cgcagccgct gcgtgagttt atgctgcgtc agacccgtga ggcggattta gggttgtttg
    10021 ccagactggc gaatactggc ccgttgcagg gacctgaagc cgtgccgatg cgcattttgc
    10081 tcccggccta cgtgaccagc gagttgaaaa ccgcatttca gataggcttc acgattttca
    10141 tccctttttt gattatcgac ctggtgatag ccagcgtgtt gatggcattg gggatgatga
    10201 tggtcccccc cgccaccatt gctctgccct ttaaactgat gctgtttgta ctggtggatg
    10261 gctggcaatt gctggtcggt tcgctggcgc agagctttta cagctagaga ggcaaaatga
    10321 cacctgaatc ggtcatgatg atggggactg aagcgatgaa agtcgcgctg gcactggctg
    10381 ccccgctgtt gctggtggcg ctggtcacgg gccttatcat cagtattttg caggccgcca
    10441 cgcagatcaa cgaaatgacg ctgtcgttta ttccaaaaat catcgccgta tttattgcca
    10501 ttattattgc cggaccgtgg atgctcaatc tgttgctgga ttacgtccgc accttgttca
    10561 ctaacctgcc gtatatcatc gggtagcccg tacaatgatg caggtgacaa gcgatcaatg
    10621 gctttcctgg ctaagtctgt acttctggcc gttactgcgc gtgctggcgc tcatttctac
    10681 cgcgccaatt ctgagcgaac gcagcgtgcc gaaacgggta aaactgggtc tggcaatgat
    10741 gatcacgttc gctattgccc catcattacc tgccaacgat gttcctgttt tttcgttctt
    10801 tgctctgtgg ctggccgtgc agcagatcct gatcggcatt gcgcttggtt tcaccatgca
    10861 atttgccttt gccgctgtgc gaaccgctgg cgaaattatc ggtctgcaaa tggggctgtc
    10921 atttgcgacg tttgtcgatc cggccagcca tctaaatatg cccgttctag cccgtatcat
    10981 ggatatgctg gcgttactgc tgttcctgac atttaacggt catttatggt tgatttcact
    11041 gctggtcgat acctttcaca ccctgccaat tggtggcgaa ccgttgaaca gcaatgcgtt
    11101 tctggcactc accaaagcgg ggagtttgat tttccttaac gggctgatgc tggcgttacc
    11161 gctcattact ctgctgctga ccctgaatct ggcattaggt ttactgaatc gtatggcccc
    11221 gcaattatcc atttttgtta ttggatttcc attaactctg actgtcggta tctctttaat
    11281 ggcggcatta atgccgttaa ttgcgccttt ttgcgaacat ttattcagtg aaatttttaa
    11341 tttgctggct gatattatta gtgaattgcc attaatataa ttccgtaacg tttatcatgt
    11401 tatcctaagg attatccgaa aaataatacc tgcgaacatc ttccaggata ctcctgtggc
    11461 gaaatatttg ttttaagctc actcacatat cgcaacattt actttacttt aagacaattc
    11521 caggcaaatt atacaacact ttacgggata gtaagtccgc ctgaaaaatc gcgagagtgg
    11581 cgcattaggt gacccatgtt gttccgttta gtcatgatga aatattcagg taaggggaat
    11641 tatcgttacg cattgagtga gggtatgcca tgtcaacgat tattatggat ttatgtagtt
    11701 acacccgact aggtttaacc gggtatctgt tgagtagagg ggttaaaaaa agagaaatca
    11761 acgacattga aaccgttgat gaccttgcca tagcttgtga ttcacagcgc ccttcagtgg
    11821 tgtttattaa tgaggactgt ttcatccatg atgcttctaa cagtcagcgt atcaagctca
    11881 tcattaatca acatcccaat acgttattta tcgtttttat ggcaattgcc aatgttcatt
    11941 ttgatgaata tctattggtc agaaaaaatt tattgataag ttctaaatcg attaaaccgg
    12001 aatctctcga cgatatcctt ggcgatattc tgaaaaaaga gacaacgata acctcttttt
    12061 taaatatgcc gacgttatca ttgagccgaa ccgaatcgag tatgttgcga atgtggatgg
    12121 caggtcaggg aacgattcaa atctctgacc aaatgaatat caaagccaag accgtttcat
    12181 cgcataaagg taatattaaa cgtaagatca aaacgcataa taaacaggtt atctaccatg
    12241 tcgtccgact gacggataat gtgactaatg gtatttttgt caacatgcgc taacacattc
    12301 tgactggtgg tttcccacca gtcaggctga ataagattac tctgctttct ccacaaagat
    12361 accgtcctga tgccctgctt cattaaagaa ccagatgccg agcgggtagt cttccagcga
    12421 aaccaggtac attgtgcctt cactaaactc ctcaactgcc agtaccacgc caggacgacg
    12481 cggaccgcca tccgttttga ctgttacccg atcattcacc ttcatcgttt tcctcctgtg
    12541 gctttgtgcc agtgtagaac aatttcgttt ttctggcagc gccaggcgcg cgcgagtgct
    12601 gattttctcg acggtctata cttaagagat gccaggcgga cttaacgact ggcggcaaca
    12661 acagagtaac ggttgcgagg aaagatgatg aaaaccgcaa aagagtacag cgataccgca
    12721 aaacgtgagg tcagcgtcga tgtcgatgcc ctgctggcgg cgatcaatga aattagcgaa
    12781 agcgaagttc atcgcagcca gaacgattct gaacacgtta gcgtcgatgg acgtgaatat
    12841 catacatggc gtgaattggc ggatgccttc gaactggata ttcatgactt cagcgtctct
    12901 gaagtgaatc gttgaatgca caataaaaaa atcccgaccc tgagggggtc gggatgaaac
    12961 ttgcttaagc aagaagcact taaaaaattc gttacaccag gaaatctgat gtgttcatca
    13021 ccttatccgc aatttttttc gctgacaaga aaatattcgc gatatgaatg attaacgcta
    13081 cttatgaata gaaatgtgac gtgcgtcacc ccttggtggc tctggcgtca acgggatcgc
    13141 tcccgaaaaa atcatctttg attatttgtg ctgttttgag aatcatcctg gtaaggggag
    13201 aactgcgcga ccatgttttc aattcaacaa ccactactgg tttttagcga tcttgatggc
    13261 accctgctgg acagtcatag ttatgactgg caaccggctg ccccctggct cagccgttta
    13321 cgcgaagcga atgttcccgt cattctctgt agcagtaaaa cttcagcgga aatgctgtac
    13381 ttgcaaaaaa cgttggggct acaagggtta ccgctgattg ccgaaaatgg cgcagtgatc
    13441 cagcttgctg aacaatggca ggatatagac ggttttccac gcatcatctc aggtattagc
    13501 catggcgaaa tcagccaggt tttaaatacg ctacgcgaga aagaacattt taaattcacg
    13561 actttcgatg atgtcgacga tgcaaccatc gccgaatgga cgggattaag ccgtagccag
    13621 gcggcgctga cgcagctgca tgaggcgtcg gtaacgctaa tctggcgcga cagtgacgag
    13681 cgtatggcac aatttaccgc tcgtctgaac gaactgggct tacagtttat gcagggtgcg
    13741 cgcttctggc acgtcctgga tgcctctgcc ggaaaagatc aggccgccaa ctggattatc
    13801 gcgacctatc aacaattatc aggcaaacgc ccaaccacac ttggcctggg cgatgggcca
    13861 aacgatgcgc ccttactgga ggtaatggat tacgcggtga ttgtgaaagg gctaaaccgt
    13921 gaaggggtgc atctgcatga tgaggatccg acccgcgtct ggcgaacgca gcgtgaagga
    13981 ccggaaggct ggcgtgaagg gctggaccat tttttctccg ctcgttaagc gttatcgctc
    14041 gcgcataccc gattacgccc ggcctgttta gcgagataaa gccgacggtc agccagtgac
    14101 tggagttgtt caaaatcata atcaccggtt tcctcgctgc tgcttacccc cagcgaggca
    14161 ctgatgcgta tcgtcgtact cttagcaatc aacatctctt tttcatttaa cttcaggcga
    14221 atacgttctg cgacttccgc agcctccgtc agactcgcgc ctggcagaat cacacaaaac
    14281 tcctcaccac cgacccgccc ggcaacgtcc tgcgcacgca aggaactgct aattaatccg
    14341 gcagcatgag aaagaacacg gtcgcccgcc tgatgaccaa agcggtcatt aatcgcttta
    14401 aaatggtcaa gatcgacctg aatgacagaa aaaggatgtt ggtgcgtctg acacaattta
    14461 gcgagcggac gggctttttc gaacagtgcg ccactgttat ataaacgcgt taaggtgtcg
    14521 tgccacgcct gccactgcaa cgagctttgc aaaacataca tgttgctgac catccggcga
    14581 atcacatacc aggagatgag taacatggtg gtaaagagcg cccacagcag agttaatgca
    14641 atgctgatac tgccgaaatc accacgcacg ccttcgctta gcgtatggac gcgcaccagc
    14701 acaccgtcga aatggtccag acgttcccag ctaacatagc gactgttcat acgaatgccg
    14761 ccccgcgtgt catgttccat tgcctgcgcc agcaaggcca gttcacgagg gtcaaaaata
    14821 ttccctgttg gatggtcagg attggaagag gtcaataatc tcagcttact gtcatagagc
    14881 tgatactcac catcgaggtt tttatcgatg gcgtttctta aaaattgctg catggtacgc
    14941 acgggaatac tcatccccag cacgccatac cagtagtttt cactgtctac cggaacactg
    15001 acggtaacct gcggttcagt attgctggcg tgttccggtt gtgaagtgaa ccagcgtacc
    15061 gcgcggtgac gattttctcg ttgcgaatgg ccgacaaacc agggttgggt gacatagccg
    15121 taataacgcg ttggtacatt gcgcgtaaat aaggtcggct gcgtcgaaac gtaaaatccg
    15181 gcacgcgaga catacatcgc ctgttcaacc atcgacgagg agttgtgcgc cagtcgcagc
    15241 aagtaaccaa cttccagtgc agcggtaatt tcattgtcga ggctttcatt ttcgcgagac
    15301 aggagattcc cctcgctgac taatgcatcc gacacaccat tgactggcag ggtgcgtcgt
    15361 cggttgagtt cgatttgcca ggcgtgctcg tcgcgatgct gttcaaactc ggtaacggca
    15421 ttacgcagag aggtgaaatc cagtggcgct acgagagctt cgcgcatgcc attacgcaaa
    15481 aagatcagtt tgtcgacgtt atactgcaaa tgcttatcga gcgcgttggc tacgttttcc
    15541 agatgattac gctggctgga gatataggca tcttccagca ccaccacttc acgccaggtg
    15601 agcaaggtgg aaaaaagcaa taccacgata aagcagagat taacgacatg accaggcccc
    15661 aggcggcgtg cgagtttttt caaccagctc tggttttcca tttttgtctc gtgctgcacc
    15721 ctgacgactc ccaacctgct tttttatgat tctggcataa cccggcgata acgtaccgca
    15781 aaacagcgat aattttataa tggtggcatg ataagacgcg actatcattc attgcaggca
    15841 ttggcacacc aggccggatg tgaaccggcc tggtgttaat gatacccttt caaacttctt
    15901 ccgcagaacg actcccctcc cccggaacaa gctcggtttc atgaaaagct tcccgcttga
    15961 ccccgtaacc gtcataccaa cgacactcaa ccataccgct ggagtatccg gtgacaatca
    16021 tccgcgggcc gccctcttta accgtaactt cctcactaac cataaagctc atactcgcct
    16081 ccttttttcg agtgaaactt gttcacctta gttgaagatg gcgaattttg cataatagcc
    16141 tgtgcgagta ttaatgtgcc tgaccgcgca ttttcgctac tgctttcact cccagcacca
    16201 cgatgccgcc gatgataaac ccaagaatca gatttaaaac agtcggtaat atcatcgcca
    16261 ccactgcact ttgctgcccg gcgaaatgtt caatggcgtg atgcagcggc gcaataccat
    16321 gtaccacaat cccgccgccg acgaggaaca tcgccagcgt gccgacaatc gataacgctt
    16381 tcatcaacca gggcgcgata atcaataacc ctttacctaa tgcctgcatc agcgcgctgg
    16441 atttttccgc cagccaatac cccaggtcat caatcttaac gataacccct accagaccat
    16501 agacgcccac ggtcactacc agcgcgatgc ctgaaagcac cagcacctga ttaagcaacg
    16561 gcgcttctgc cacaatcccc agcgtgatgg cgacgatttc cgcagacaat ataaaatcgg
    16621 tacgaatcgc cccttttatt ttgtcctttt caaacttcag cggatcctgc gccgccagct
    16681 tctccagacg ctgctgactc tgcgccggat cttctttatg tttacgcgcc tccagcatat
    16741 gcagcacttt ctctacccct tcaaagcaga gaaacgcgcc gccaatcatc aacagtggcg
    16801 taatcgccca cgggataaac gcactgatga tcagcgccag cggcaccaga atcactttat
    16861 tgatcagcga tcctttcgcc acgccccaga ccacgggaag ttcccggttg gcccgcacgc
    16921 ctgaaacttg ttgcgcattg agcgataagt catcccctaa tacaccggcg gttttcttcg
    16981 ccgccagttt gcccatcacg gaaatatcgt ccagcagtgt ggcgatatca tcgagcaacg
    17041 tcagtaaact actcccggcc aaaagcattc cctctcattt ttattgttga agggtgtaag
    17101 tatgaagcaa aaacggcagc tctgatactt gcttcacagg tcaacatttt tttaacaatc
    17161 aagtgcaatt atatctctcg ccagcagaat gattttcacg tttactatga cccacctttt
    17221 tatttccacc gtgagggatt atgcgtttcc gccagttgtt accgcttttt ggcgcgctgt
    17281 ttgcgttgta tatcatttgg ggctcaacct attttgtcat tcggattggc gtggaaagct
    17341 ggccgccgtt aatgatggca ggcgtgcgat tcctggcagc cggtatttta ttgctggcat
    17401 ttttgctact gcgcggacac aaactccccc cgctacgtcc gctgctcaat gccgcgctga
    17461 ttggcctgtt attgctggct gtcggtaatg gcatggtaac ggttgccgaa catcaaaatg
    17521 ttccttccgg catcgccgcc gtagtggttg caaccgtgcc cctctttacc ctgtgcttca
    17581 gccgcctgtt tggcattaaa acgcgcaaac tggaatgggt gggtattgcc attgggcttg
    17641 ccggaatcat catgctcaat agcggtggaa atttaagcgg caatccgtgg ggcgcgattc
    17701 tgattttaat cggctcgatt agctgggcgt ttggctcggt ttatggctcg cgcattacct
    17761 tacctgtagg gatgatggcg ggtgcgattg agatgctggc ggcaggcgtg gtgttaatga
    17821 tcgcgtcgat gattgcaggt gaaaaactga cggcactccc ttccctttca ggcttccttg
    17881 cggtcggcta tctggcgctg tttggttcga ttatcgccat taacgcttat atgtatttaa
    17941 tccgcaacgt cagtccggct ctcgccacca gctacgccta cgttaacccg gtggtcgcgg
    18001 tcttgctggg tacgggactg ggcggagaaa cgctgtcgaa gattgaatgg ctggcgctcg
    18061 gcgtaattgt cttcgcggtg gtactggtca cgttgggaaa atatctcttc ccggcaaaac
    18121 ccgtagttgc gccagttatt caggacgcat caagcgagta aatgaatccc ctgcgtgtcg
    18181 atctgcgcgc tggcaccttc gccgcagatc cactcttcca gacgctcgct aagcgcttca
    18241 tccgtcagct tctcgcgccc acgtaatgca cactcccaga caatcaatac gcgacagccg
    18301 agttcctgca aacgactgat atcgcggcga tcgcgttcaa catttttacc tatcttctcc
    18361 agccagaact cggttcgagt cgcaggcact ttaaacagat agcagtgatg atgatgccag
    18421 aagcagccgt gggtaaatat cacgcagcga tattcatcaa cgacaaaatc cgggcgtccg
    18481 ggcagactgg cgtcctgaac gcgaaacgcc aggcccagcc cggttaacag actggcgagg
    18541 cgcttctcta tcgccgtatc acgcgtggca atcgcgcgca tatttttgct gcgagtggcc
    18601 ttatcgtgaa cgtcggccat gttgtgcctc ttgctgacgc aacgccaccg cctgtttgat
    18661 ttttggctca agcagttttg ccaccgcggc aaagaccggc acgaccaccg aattaccgaa
    18721 ctggcgatag gcctgagtgt ccgaaaccgg aatgcggaat ttcgcttctc ccggcgcttc
    18781 aaaacccatt aagcgcgcgc attcccgagg cgttaaccga cgtggacgat gttgctgatt
    18841 cagcggatcg tcaaaatctt tctcacccgt ggccatatcc cagccgcgat cgatcaaaat
    18901 ttccgcgcca tctttgtaat aacgcgcaga caacgtacgc gtgacgcttt gcggattgtt
    18961 cggataaacc attccataac cgaagccatt accgcgtgcc tgatgttttt tcgcataacg
    19021 atagaggtac ttccacagca ccggcgtcag gatatatttc gcctcgacca tcgggtccag
    19081 caactgggcc agcgtcactc gctgcgcagg gaaacattcg ctgatatcac gcagagtaaa
    19141 atcggctttc agattcagat cgcgacgaaa acccaccagc acgatgcgtt cacggtgctg
    19201 cggcaggaaa tgtttgccat cgatgatttt cggatcgtcc ggcccgttat cttcagcgtc
    19261 ggcaacgtca tagcccagtt cgtcaagcgt ctgcatgatg atgcggaacg ttttaccctg
    19321 gtcgtggctt ttcaggtttt tgacattttc gagcacaaaa atcgccggtc gacgcgcgtc
    19381 gataatacgc accacatcaa agaacagcgt gccctgggta tcacaggcaa aaccgtgcgc
    19441 ccgcccgagc gagttctttt tcgatacgcc agccagcgaa aatggctgac aaggaaaacc
    19501 ggccagtaat acatcgtgtt caggaatgtg ttgacgaata tgttccgctg ccgcctcatc
    19561 actcacgccg tctttatggc tgagggtgat gtcgcggata tcttcattaa aatgatgcgt
    19621 cgccggatcg caataatggt tggctttata agtgcgaacc gcatgtttgt tccattcgct
    19681 ggtaaagacg cactgtccgc caatcgattc aaaaccgcga cggatgccgc caattccggc
    19741 aaatagatcg ataaagcgaa acgcataatg cggatgatgt gccggaggtt tgggtaacaa
    19801 cgtttgcaga tgggcgaact ccttctcact taaacggtgc catgccgagt cattcgccag
    19861 cgcacgtttt aaaattgccg cgctccagtg attctcaccc acaccattaa gctgcgccac
    19921 cagcgtttta acatcataaa tttgcagcag tttctccagc attgcctgtg cggcattccc
    19981 ggtgctgtat gaatcggtta ctgatatatt ttcctgcata gatttaaccg gccatctgag
    20041 atggccgaca ggttaacata attacagcca tttagggcca cgcgctcagc ccgcgggtga
    20101 aaacgcatct atcaccttgt gatcgacacc ttcattctcc cccgccagtt cggcactgag
    20161 cttcgccata aactcgacta aaaattgcgc gttgtgctgc gccagctgct tgcctcttgc
    20221 ggtttgcatg gtttgcggta gcttcagcaa cttagtctgg aaatgatcca gcgcgtagcg
    20281 tttatcatca agcgggcgat gctgtgcgaa cggatcttca ccatcaaaca gcgccacgcc
    20341 caatgcccct gaaacggcaa acacgcgcgc caggccaatc gcccccaggg cttccagccg
    20401 atccgcatcc tggacgattt tagcttccgt tgttaagggg gcgatttgcg cgctgaaact
    20461 gtgagcggca atggcatgac aaacggcctc gattttctcc gccggaaatt gctcgaactc
    20521 ttcacggagc aggcgacgcg tctcttctgc cgccaggatt gaagaacgct gccgttgcgg
    20581 atgatttttc gccaggctca caatatcgtg gaaataacag gcggttaaaa tcaccagcat
    20641 atcgacgtca tcatctgccg ccagcttctg tgccgtagcc cagacgcggc gaaaatgata
    20701 cacgtcatgg gccgcgtcct ggtgctgatg atggtttttc aaccagtttt cgaactgtgt
    20761 ctgccagtgt tgtaagtcca tacgcgctcc gttgccaaag agtcgccacc ttagcagttt
    20821 ttattcatag cgtctgctac gtgcgggacg aggtcgggtg taccaggcaa tgctgcctaa
    20881 caatgccccg accagagcca taaataaaaa tccgaccagt gcccctaacc actttcccgc
    20941 ggtagagcct aaatcgcttt caaatcccga cacgggggca tccggctcac ccccagatgt
    21001 aacgcgccat tgcccagacg aaatagccac accatgtata gaaagcccac aaagccagtt
    21061 tgccaccagg gctgcgagag agcctctctt ccatctttgt cgaaccgtat tccattttga
    21121 gataacccgg aaaattcaga cttagtacta ttatgtgata aaagtcacat ttttccacat
    21181 tgaatgagcc agtcaagact caatcatttt ttgaacaaat ag
//
LOCUS       DACBVI010000072        21174 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.072, whole genome shotgun sequence.
ACCESSION   DACBVI010000072 DACBVI010000000
VERSION     DACBVI010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.072"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(498..727)
                     /locus_tag="IU325_13060"
                     /pseudo
     CDS             complement(498..727)
                     /locus_tag="IU325_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1613..2494
                     /locus_tag="IU325_13065"
                     /pseudo
     CDS             1613..2494
                     /locus_tag="IU325_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118119.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(2811..4076)
                     /locus_tag="IU325_13070"
                     /pseudo
     CDS             complement(2811..4076)
                     /locus_tag="IU325_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            complement(4262..4346)
                     /locus_tag="IU325_13075"
     tRNA            complement(4262..4346)
                     /locus_tag="IU325_13075"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4310..4312),aa:Leu,seq:caa)
     gene            4542..5561
                     /gene="ahr"
                     /locus_tag="IU325_13080"
     CDS             4542..5561
                     /gene="ahr"
                     /locus_tag="IU325_13080"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HAP5809950.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADY"
     gene            complement(5565..6128)
                     /gene="idnK"
                     /locus_tag="IU325_13085"
     CDS             complement(5565..6128)
                     /gene="idnK"
                     /locus_tag="IU325_13085"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP5809951.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQNRICAKEGSASDQRCE"
     gene            6345..7376
                     /locus_tag="IU325_13090"
     CDS             6345..7376
                     /locus_tag="IU325_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP5809952.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALRFAGDKT
                     QTAKVQLVF"
     gene            7400..8164
                     /gene="idnO"
                     /locus_tag="IU325_13095"
     CDS             7400..8164
                     /gene="idnO"
                     /locus_tag="IU325_13095"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP5809953.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTSVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            8229..9548
                     /gene="idnT"
                     /locus_tag="IU325_13100"
     CDS             8229..9548
                     /gene="idnT"
                     /locus_tag="IU325_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HAP5809954.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGHYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            9615..10613
                     /gene="idnR"
                     /locus_tag="IU325_13105"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             9615..10613
                     /gene="idnR"
                     /locus_tag="IU325_13105"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HAP5809955.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSEHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLEKRVRHKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            10691..12193
                     /locus_tag="IU325_13110"
     CDS             10691..12193
                     /locus_tag="IU325_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HAP5809956.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDVNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASTEQQNNPPAKGKEIAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(12354..13436)
                     /gene="lptG"
                     /locus_tag="IU325_13115"
     CDS             complement(12354..13436)
                     /gene="lptG"
                     /locus_tag="IU325_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAP5809957.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(13436..14536)
                     /gene="lptF"
                     /locus_tag="IU325_13120"
     CDS             complement(13436..14536)
                     /gene="lptF"
                     /locus_tag="IU325_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAP5809958.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            14803..16314
                     /gene="pepA"
                     /locus_tag="IU325_13125"
     CDS             14803..16314
                     /gene="pepA"
                     /locus_tag="IU325_13125"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HAP5809959.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            16668..17111
                     /gene="holC"
                     /locus_tag="IU325_13130"
     CDS             16668..17111
                     /gene="holC"
                     /locus_tag="IU325_13130"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HAP5809960.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            17111..19966
                     /gene="valS"
                     /locus_tag="IU325_13135"
     CDS             17111..19966
                     /gene="valS"
                     /locus_tag="IU325_13135"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP5809961.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVVLRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLTNEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(20022..>21174)
                     /locus_tag="IU325_13140"
     CDS             complement(20022..>21174)
                     /locus_tag="IU325_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HAP5809962.1"
                     /translation="VFHGTGERYFLICVVNVLLTIITLGIYLPWALMKCKRYLYANME
                     VNGQRFSYGITGGNVFVSCLIFVFFYFAILMTVSADMPLVGCVLTLSLLVLLIFMAAK
                     GLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTVFFISTKMLHANSSSS
                     VIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFKVKLDTTYCIKYAILA
                     FLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQRKMIIAQLIYYFGIA
                     VSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAYPLL
                     KIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIMPSLPFL"
BASE COUNT         5427 a         5263 c         5344 g         5140 t
ORIGIN      
        1 aaccaagatg cctatttcgt ttcagcatca actgttaaat tatctggttg ccaacgagaa
       61 tactgagctt ttttgtcccc aagactgacc gtcaacgcca caatctttga ctgtttgaca
      121 ttgtcatgcc cgcaggccag cggatctaaa gacattagac tgcttgcatc agccagttgc
      181 gtatctggct atgtccctta attggtaagg gttcgaggaa ctcaacatta acgcattttt
      241 tagcgcatca ccgacagtgt atatgaacga ttgcactctg acgatggttg atttatttgg
      301 ttgcgtaggg gctagctagt ctggtaccgg aggctagatc tgaagtactg accccaggaa
      361 taacgccggg ctaactcata ttctgaccca ttgtcagccc gcgtgtttac tgctctagct
      421 catttcctgt tgtcaggcgt tcaattccca actgcaggtt atggcccggt gcacaaacac
      481 ctgacggcag gtatcactta cccgaagaag gtaaagccct caagaggcag taactcagcc
      541 aacctctcct ccggccactc cggcagacgc gtcaggacgt ccgtcagcca agcatgtggc
      601 tccagactgc ggttcccaga aggctcatta tctgtgccgc gcggttcccg gccaccagtg
      661 aaccagcgaa caaccaggcc tttcgtccca taacgaccgg acggatagca cgctcgcaga
      721 tattgttgtg tgtgccttga aatgcacgat ctttccgtcg gtgaaagtcc gccccggcta
      781 actctcgctc cgactggaag gacaagaagc ggtccaggag gtaacaaaag ggctgaatct
      841 ctccctgaaa cgacttcttc aggaagtcaa cgggttatgc gggccgtaac gtgagtgaac
      901 actgagcagc catcgaaatg ccgtcgaggg tgctgacacg ccacaataac ggggaaggcc
      961 gacgatgacc gggaagagag tgagaaaggc atcggtcata cccaccgggg ttgtggtgtc
     1021 agcatgcata acaattgatc gatcgtgcaa caagagaagt ccataacggt gtctgtccgg
     1081 gtgacgggca acaacgcctc gcgagggggt gaatcggttg ttatggatgg cagatggggt
     1141 gtagtagtga aaatactgag taatgtcaga ggagcgaagg ccccctcttg cagtaccagt
     1201 gtaacatgca ggaagagccc ggcgattgcc atcagcctca caaccccgct agaaccggtt
     1261 cagaacctac agaaaacact acaggagaaa gcaaagtgaa ccgccccgtg tttcctgttt
     1321 atcattttct ggtcagtgcc gcgattctgg tattcgtggt gattttctgg cggacacacc
     1381 atcgcgacca ccgtaactgg ctggctctac ggctattcgt attatgctct gtaaaccgtt
     1441 ggccattgcg gatggttaaa ggaacagttg tggggacaac tgacacatta cgtgaaatgc
     1501 agcgttatga acaactgagt agcacggggc tgacaactgg aaaatccagc cgggagcacc
     1561 gttatatgat acgattgtta ttgtcactgg tgagagtgtg cgcagggatt atatgtcagt
     1621 gtatgactat cccgtaccaa ccacaccgtg gctgaatacg gcacccggtt tatttattga
     1681 cgattatacc tcgacagcct ccagtacagt gtcttccctg agccggacac tgatttatga
     1741 ctatgagcag aaccctgatt ccggcaacaa tgtggtggcg ctggcagcaa aagcaggata
     1801 cagcacatgg tggatatcca atcaaggaaa actgggggag catgacacac gcatctctgt
     1861 tattgcttct gatgcggagc atgccacttt cctcaagaaa ggcagcttcg cttcccgtaa
     1921 aacagatgac aaactgttgt tacaggaaac agaacgtgcg ctggcggata catcctctcc
     1981 gaagataatt ttcctgcaca tgatgggttc tcatccaaat ccgtgtgaca gccttaactc
     2041 ctagccgaat aattacctgg agcagtatcc ccgaaaaatt gcctgttacc tcgccagcat
     2101 cagtaaactg gataactttc ttggccagct tgatggtatc cttcgccggt actcacgtca
     2161 tttcgccatg ctttactttt ctggccttgg gctgtcggtc agcgacagtg ccaatcctgt
     2221 tcatcattat ggtcatgtgc agggaggcta cagtgtgcca ctgattatta ccgccagtga
     2281 cataacgtct catcagcccg tcagtagaaa aatcagtgcc cgtcatttcg caggtatttt
     2341 tcagtggatg accggtattt gtactgaaaa tataccgcca ttcaatccgc tgacagacga
     2401 agataactaa cctgttatgg tttttaaggg agcgaggaat ataccggcag acagtttgaa
     2461 acctcagcca cttattcttc ctgatcacag gtaatcatat atggcagact gtaatacagt
     2521 cgcttgtact gaaaagtatt ctgtataaaa tatccgtatt catatcagca caagggacca
     2581 tcaaatccct gcagcactcc ttacgtcagt tatcccgcat caccctgtga gtacaggatt
     2641 ttttatgagg ttactttttt attgaatgta tataaataat gaataaccgc ggattctgat
     2701 aatcccccct gcagggagcg gtaaatagta aatacatcag ttaataccgt ttgtcttttt
     2761 taacaaagaa aataatccta gaataattcc cgggcatttg cccgggatga ttactgtttt
     2821 aatggattat taatctttgc atattcaaat ggactgataa acctttctct gtttacatcc
     2881 agaaaatcgg cccaccactg tagcatcaat cgccgttctt ccagatgctc tgctttatgg
     2941 atatacgcgg ccctcactga atttcgcgcc atgtggctca tctgacgttc aacagcatca
     3001 cgagaccaca gacctgattc gaccaatgaa ctacaggcca ttgttcgaaa gccatgacca
     3061 caaacctcta cttttgtatc ataccccatg acccgtaacg cactatttac cgtattctca
     3121 ctcatgggtt tgtgcgaatc gtgatcacca ataaatatca agtcatgggc cccataaaac
     3181 tgttttatct gctttaaaat tgcaagagct tgcgttgaaa gaggcactag atgcgttgta
     3241 cgcatttttg agcctctatg ggaatgtttc actccaggaa taggctcccg ctccggtggg
     3301 atagtccata tagacgcttc gaaatcgatc tctgaccaac gagcaaaacg cagctcactg
     3361 gaccgaataa agatcagcaa agtgagttct atcgcccatc gggttagcgg cctaccagta
     3421 tagctatcta tttttgtaag caactcaggg atgcgcttta attcaagcgc gggacgatgt
     3481 tgtcgattac aggaagcaac cgccccagcc atctcttgtg ccgggttata atcaattaac
     3541 ccactttgca ctgcatagcg catgatggct gtagtgcgct gctgaagacg agcggccact
     3601 tcaagacgtc cagacatttc tacggcctta ataggtgcta ataaatctcg agtttttaac
     3661 tcagcgatat tacgttcacc aagcgctgca aaaagattat cttcaagact ttttagcaca
     3721 cgatgggcgt gatcttcaga ccacttttta ttggtgccat gccactcaat cgcgacttct
     3781 ttaaaggttc gtgctttact ctgttcaacc ttatcatttt tctttttgtc tcccggatcg
     3841 acgccattcg caagcagctt acgcgtctcg tcacgacgta ctctggcatc cgctagtgtg
     3901 atttcaggat aaaccccaag tgccagcatt ttttgctttc cctcataacg gtactgcaaa
     3961 cgccagtact tagaaccatt tggatggaca agcagatgca taccgccacc atcagtcagc
     4021 taatattgct tatccgttgg cttggctgct ctgactttga tatctgttaa tgccatcgta
     4081 ttccctccct gttggtacaa gcattatcga accggaaata ccatcatctg taccaacata
     4141 tgttggtaga tgtacgttga tatcggttga ccttgagaga gttaatatag cgggaaagat
     4201 agataaacac tggatttcag gcataaaaaa agacctcagt tgaggtctat ttacatactt
     4261 ttggtgccga aggccggact cgaaccggca cgtatttcta cggttgattt tgaatcaact
     4321 gcgtctaccg atttcgccac ttcggcactg aagaggtatg cggaaaacgt tgtggattat
     4381 acctgtcgca cgccaccatg caagcgccag cttgctcgca cctcgctaag tgctgaaatt
     4441 ttcagcattg catacagcga tgtgtaacct ttgtcacact ccaggcaccc cgccctgcca
     4501 tgctctacac ttcccaaaca acaccagaga aggaccaaaa aatgtcgatg ataaaaagct
     4561 atgccgcaaa agaagcgggc ggagaactgg aagtttatga gtacgatccc ggtgagctga
     4621 ggccacaaga tgttgaagtg caggtggatt actgcgggat ctgccattcc gatctgtcga
     4681 tgatcgataa cgaatgggga ttttcacaat atccgctggt tgccgggcat gaggtgattg
     4741 ggcgcgtggt ggcactcggg agcgccgcgc aggataaagg tttgcaggtc ggtcagcgtg
     4801 tcgggattgg ctggacggcg cgtagctgtg gtcactgcga cgcctgtatt agcggtaatc
     4861 agatcaactg cgagcaaggt gcggtgccga cgattatgaa tcgcggtggc tttgccgaga
     4921 agttgcgtgc ggactggcaa tgggtgattc cactgccaga aaatattgat atcgagtccg
     4981 ccgggccgct gttgtgcggc ggtatcacgg tctttaaacc actgttgatg caccatatca
     5041 ctgctaccag ccgcgttggg gtaattggta ttggcgggct ggggcatatc gctataaaac
     5101 ttctgcacgc aatgggatgc gaggtgacag cctttagttc taatccggcg aaagagcagg
     5161 aagtgctggc gatgggtgcc gataaagtgg tgaatagccg cgatccgcag gcactgaaag
     5221 cactggcggg gcagtttgat ctcattatca acaccgtcaa cgtcagcctc gactggcagc
     5281 cctattttga ggcgctgacc tatggcggta atttccatac ggtcggtgcg gttctcacgc
     5341 cgctgtctgt tccggccttt acgttaattg cgggcgatcg cagcgtctct ggttctgcta
     5401 ccggcacgcc ttatgagctg cgtaagctga tgcgttttgc cgcccgcagc aaggttgcgc
     5461 cgaccaccga actgttcccg atgtcgaaaa ttaacgacgc catccagcat gtgcgcgacg
     5521 gtaaggcgcg ttaccgcgtg gtgttgaaag ccgattattg aaaatcattc gcagcgctga
     5581 tctgaggcgc tgccctcttt cgcacatatt ctgttttgtc gtatcgccag cacagcctgc
     5641 cgacattgtt cggtgacatt ggcaatatca tggttgatat caatgcgcac aatatcctgt
     5701 tcatctgctt gtggacgctc cagtgcctca aactgacttt ttagtaacgc taccggcata
     5761 aaatgcccag cccgacgctg cattcgcgcg agaatagttt catagtcgcc atctaaccag
     5821 aggaaatgaa catgggggct acccttgcgt aaaatatcac gatactgttt ttttaatgat
     5881 gaacagacaa taaatcctgt ttcattcttt ttataaagac tgtatgaagc atcatttaag
     5941 cgttccagcc agggaagtcg atcttcatca gataatggaa taccctgcga cattttatct
     6001 atatttttgg ctggatgaag atcgtcacca tcaataaatt tagcagataa taacgcggca
     6061 accttgctac caattaatgt tttaccactc cctgaaacgc ccatcaaaat aaagctttca
     6121 cccgccattt aaaatctcct gaatcatctt acctgctgcg atactacgcc tggcggcata
     6181 ataatttcac gtttttttat ttctactgat aagaattaca aggcacatca cgttatgcgt
     6241 aacatagtaa tgtaacaatt ttctgacgtg atcttcatca caaataatga cagttaaaac
     6301 cgcttaaatg cttccgaggt gtctacctga ccagtgagga atttatgcaa gtgaaaacac
     6361 agtcctgcgt tgttgcgggc aagaaaactg ttgccgttac cgagcagacg atagattgga
     6421 ataataatgg aacattagta caaataaccc gaggtggaat ttgcggttcc gatttacatt
     6481 attatcagga aggaaaagta ggtaatttca tgataaaggc accgatggtg ttaggtcatg
     6541 aagttatcgg taaagttatt catagcgact catcagaatt acatgaaggg caaacggtag
     6601 ccattaatcc gtctaaaccg tgcggtcact gcaaatactg cattgaacat aacgagaatc
     6661 agtgtacaga tatgcgtttt tttggcagtg ccatgtattt ccctcatgtt gatggtggtt
     6721 ttacccgtta taaaatggtc gaaacgtcgc aatgtgtccc ttatccggcc aaagctgacg
     6781 aaaaggttat ggcttttgcc gaacctttag ccgtcgcgat tcatgccgca catcaggccg
     6841 gcgagttaca gggcaagcga gtatttattt ctggtgttgg gcccattggc tgcctgattg
     6901 tcagtgctgt gaaaacactg ggggccgcgg aaattgtctg tgccgatgtg agtccccgtt
     6961 ccctttcgct gggtaaagag atgggggcgg atgtgctcgt aaacccacaa aacgacgaca
     7021 tggatcactg gaaagcggaa aaaggctatt tcgatgtcag ctttgaagtg tccggtcatc
     7081 cttcatcagt gaatacctgt ctggaggtca ctcgtgcacg cggcgtaatg gtgcaggtag
     7141 gtatgggagg cgcgatggca gaattcccaa tgatgacatt gattggtaag gagatttcac
     7201 tcaaaggctc tttccgtttt accagcgaat ttaataccgc agtgtcatgg ctggcgaatg
     7261 gcgttatcaa tccactgcct ttactgagtg ctgaatatcc cttcactgac ctggaagagg
     7321 cgctacgttt cgccggtgat aaaacccaga cagcaaaagt ccagcttgtt ttctaagaga
     7381 taaataaagg aataatacaa tgaacgatct attttcactg gcaggaaaaa atatcttgat
     7441 taccggttca gcacagggca ttggcttttt actggcaacc ggcctgggta aatatggcgc
     7501 acaaataatt attaatgata ttactgccga acgcgcagaa cttgctgtag aaaaactcca
     7561 ccaggagggt attcaggccg ttgccgcacc ttttaatgtt actcataaac atgaaattga
     7621 tgccgccgtt gaacatatcg aaaaggacat cggccccatt gatgtgctgg tgaataacgc
     7681 cggtatccag cgccgtcatc cttttactga gttccctgaa caagagtgga atgatgtgat
     7741 cgcagtaaac cagacctccg tgttcctggt atcgcaagcg gtaactcgtc acatggttga
     7801 acgcaaggca ggtaaagtta ttaatatttg ctcgatgcaa agcgaactgg gacgtgacac
     7861 catcacccct tatgccgcat cgaaaggggc ggtaaaaatg ctcacccgcg gcatgtgcgt
     7921 cgagctggcg cgccacaata ttcaggtcaa cggtattgcg ccgggctatt tcaaaacaga
     7981 aatgactaaa gcactggttg aggacgaagc cttcaccgcc tggttgtgca aacggacccc
     8041 cgccgcacgc tggggagatc cgcaggaact gattggtgct gcggtgttcc tttcttcaaa
     8101 agcctctgat ttcgtaaacg gccacctgtt gtttgttgat ggcggcatgt tagtggctgt
     8161 ttaacaccta ctaccctacg tgcagcgcga aagcgctgct tttctggcac taataagaga
     8221 taaagactat gccattaatc attattgcgg caggcgtcgc gctgcttctt atcctgatga
     8281 tcggctttaa agttaacggc tttattgccc tcgttctggt agctgccgtc gtcggatttg
     8341 ccgaagggat ggatgcacag gccgtcctgc actctataca aaatggtatc ggcagcacgc
     8401 tcggtgggct ggcaatgatc ctcggtttcg gggccatgtt aggcaagctg atttctgata
     8461 cgggtgcggc acaacgtatc gccactacgc tgattgctac ttttggtaaa aaacgcgtgc
     8521 aatgggcgct agtgatcact ggtctggttg tgggcctcgc catgtttttt gaagtgggtt
     8581 ttgtcctgct gttgccgttg gtatttacca tcgtagcatc atcaggatta cccctgttgt
     8641 atgttggcgt accaatggta gcagcgctct ctgtaaccca ctgttttctg ccgccacatc
     8701 cagggcctac tgccatcgcg actatctttg aggctaatct cggaacgact ttactgtatg
     8761 gatttatcat taccattccg acagttattg tcgcaggacc gctgttttct aaactgctaa
     8821 ctcgctttga gaaagcacca ccggaaggct tatttaatcc tcatctgttt agcgaagagg
     8881 agatgccctc cttctggaac agtattttcg ctgccgtgat cccggtcatc ctgatggcta
     8941 tcgccgccgt ttgtgaaatt acgttaccga aaactaacac cgtgcgcctc ttctttgaat
     9001 ttgtcggtaa ccctgccgtt gcgctgttta ttgccattgt tattgcgatt ttcacactgg
     9061 gccgacgtaa tggacgcacc atcgagcaaa tcatggatat cattggggat tctataggcg
     9121 ctatcgcgat gattgtgttt attatcgctg gcggcggcgc gtttaagcag gtattagtag
     9181 atagcggtgt cgggcactat atttcacact taatgaccgg aactacgctt tcgccgttat
     9241 tgatgtgctg gactgttgcg gcgctgttgc gtatcgctct gggctctgcc accgtcgcgg
     9301 ccattaccac cgcgggtgtg gtgttgccga ttatcaacgt tacccatgcc gatcccgctt
     9361 taatggtact ggcaaccggt gcgggcagcg tgatcgcgtc acacgtaaac gaccctggct
     9421 tctggctatt taaagggtat tttaatctga cggttggtga aacgttgcgt acctggacgg
     9481 tgatggaaac ccttatttct attatgggtt tgctgggcgt gttagccatt aacgccgtat
     9541 tgcactgaaa gaataaaata ttgtgatgta gtttcggggg aagattgaag attcccccga
     9601 cgttgggaga agttatgcgc aatcacagaa tttctttaca ggatatcgct acgctggctg
     9661 gcgtaacaaa aatgaccgtg agtcgttata tccgctcgcc gaaaaaggtg gcaaaggaaa
     9721 caggcgagcg catcgccaaa attatggaag agatcaatta tatccctaat cgcgctcccg
     9781 gcatgttgtt gaatgcacaa agttataccc tcggtatctt gattccgtca tttcaaaatc
     9841 aactcttcgc tgacattctc gcgggaatag aatccgtcac ctctgagcat aattatcaga
     9901 cccttattgc taattacaat tacgatcgcg attccgaaga agagtcggtg attaacctgc
     9961 tctcttataa cattgacggg attatacttt cggaaaaata tcacaccatc aggacggtga
    10021 aatttctgcg ttcagccacc attccggtcg tggaattgat ggatgtacag ggagaacggc
    10081 tggatatgga ggtcggtttt gataatcggc aggccgcttt tgacatggtg tgtaccatgc
    10141 tggagaagcg agtcaggcac aaaattctgt atctcggttc caaagatgac acccgcgatg
    10201 aacagcgtta tcaggggtat tgcgatgcga tgatgctgca taatctttcc ccattacgca
    10261 tgaatccacg cgccatctca tccattcatt taggaatgca gttaatgcgt gatgccctta
    10321 gcgcaaaccc ggatttagac ggtgtatttt gcaccaatga cgatattgcg atgggtgcgc
    10381 ttctgctgtg ccgcgagcga aacctcgccg tgccagaaca aatttccatt gcgggttttc
    10441 atgggctgga gattggcagg caaatgatcc caagcctcgc cagcgtaatt actccccgtt
    10501 ttgatattgg tcggatggcc gcccaaatgt tgctgagcaa aattaaaaat aacgatcaca
    10561 accacaacac tgttgacctc gggtatcaga tttatcacgg caacacgctt taggttattt
    10621 tctcttcatt acacgtattc tctttgccgg acgttgccta tactccaggc aggaaactgg
    10681 aggatatctc atgagcgaac ccctgttaat tgcccgcacg ccggacacag aactattttt
    10741 actgccggga atggctaacc gtcacgggct gattactggc gcaacgggga cgggtaaaac
    10801 tgtcacgctg caaaaactgg cggagtctct atcggaaatc ggtgttccgg tgtttatggc
    10861 cgatgtgaaa ggcgatctga ccggcatcgc gcaggcagga acggcatcgg aaaaactact
    10921 cgcaaggctt aaaaatatcg gcgtcaatga ctggcaaccg catgccaatc cggtggtggt
    10981 gtgggatatc tttggcgaga aaggccatcc ggtgcgggcg acggtttcgg atctggggcc
    11041 gctgttgctg gcgcggctgt tgaatctcaa cgatgtgcaa tctggcgtgc tgaatatcat
    11101 tttccgcatt gctgacgatc aggggctgtt actgctcgac tttaaagatc tgcgggcaat
    11161 tacccagtac atcggcgata acgccaaatc cttccagaat cagtacggta atatcagtag
    11221 cgcatcggtt ggtgccatcc agcgcgggct gttatcgctg gaacagcaag gcgcagcaca
    11281 cttctttggt gagccgatgc tggatatcaa agactggatg cgtaccgatg tcaacggtaa
    11341 aggcgttatc aatatcctca gcgccgagaa gctttatcag atgccgaaac tgtacgccgc
    11401 cagcctgctg tggatgcttt cagagttgta tgaacaattg cctgaagcgg gcgatctgga
    11461 gaaaccaaaa ctggtgtttt tcttcgacga agcacatctg ctgtttaacg atgcgccaca
    11521 ggtactgctg gataagattg agcaggtgat aaggcttatt cgctcaaaag gcgtgggcgt
    11581 ctggttcgtt tcgcaaaacc cgtcagatat tccggataat gtgctcgggc agctaggtaa
    11641 tcgcgttcaa cacgctttgc gggcttttac gcccaaagat cagaaagcag taaaagctgc
    11701 ggcgcaaacc atgcgggcca atccggcgtt tgataccgaa aaggcaatcc aggaactggg
    11761 gaccggtgag gcgttaatct cgtttctcga tgcgaaagga agtccttcag tggtggaacg
    11821 ggcgatggtt atcgcgcctt gttcgcgaat ggggccggtg acggaagatg agcgtaacgg
    11881 cttgattaat cactccccgg tgtatggcaa gtacgaggat gacgtggacc gtgaatccgc
    11941 ctatgagatg ttgcaaaaag gctttcaggc cagtaccgag cagcaaaata atcctcccgc
    12001 gaaagggaaa gagatagcgg tagatgacgg cattcttggt ggattgaagg atattttgtt
    12061 cggcactacc ggaccacgcg gcgggaagaa agatggtgtg gtgcaaacaa tggcgaaaag
    12121 cgccgcccgc caggtgacga atcagattgt acgtgggatg ttggggagtt tgctgggggg
    12181 aagaagaagg taagtcaggc gcgatttgtc tttgcctgat gcgacgctgg cgcgtcttat
    12241 catgcctaca ccactgcaat atattggatt ttcatgattt ttcaggcatg aacgaagcac
    12301 actttgctaa caataataaa gggagctttc gctcccttta ttcgttcatt cggttacgat
    12361 tttctcatta acagccacag gctgattaag aagaagctgg cgcttggcaa cagtgcgccg
    12421 atgatcggcg ggatgccata aaccaacgtc agcgggccga agatctggtc cagtacgtag
    12481 aagacaaaac cgaagctgat accggtgacc acacgcacgc ccatcggtac gctacgcagt
    12541 gggccaaaga tgaacgacag cgccatcagc atcatcaccg ccacggatag cggctggaag
    12601 attttgctcc acatgttgag ctgataacgt ccagcgtcct gaccgctcga cttcagatac
    12661 ttcacgtagt tgtgcaaacc gctgattgag agtgcatccg ggtccagcgc caccacgccc
    12721 agtttgtctg gcgtgaggtt ggttttccag gtgccgctca ccgtctgcga accggtaatc
    12781 tgtttcggat tggtcagatc agattcatca acctgcgaca gacgccagac tttatgttcc
    12841 gggtcaaact tcgcagtagc ggcatagcgt acggattgca gacgacgatt ctcgttaaag
    12901 gcataaatgc tgatgccacc taactcttcg tcacctttaa cccgctcaat gtagacgaag
    12961 ttgttgccat ctttcgccca taaaccttgc tgggtagaga gcaacgagcc gccgtacatc
    13021 gcctgcgcac ggtagttacg cgccatctgc tcgccctgcg gcgcgaccca ttcgccaatc
    13081 gccatcgtca gcaagaccag cggaatggcg gttttcatca ccgacagcgc cacctgcata
    13141 cgggtaaaac cagaagcctg catcaccacc agttcgctgc gctgcgccag catcccaaga
    13201 ccaagcaacg ccccaagcag agccgccatc gggaagaaga tttgcacatc tttcggcacg
    13261 ctcagcaagg tatacattcc tgcgcctaac gcgtcgtaac tcccctgccc ggcttttttc
    13321 aactgatcga caaatttgat aatgcccgac agcgacacca gcatgaacag cgtcatcatg
    13381 atggtggtga aaatagtttt accgatatag cggtcaagta cgccaaaagg ttgcatcaca
    13441 ccgctccttt acgcgaaaaa ctggcgcgca ggcggcggac cggcacggtg tcccaaaggt
    13501 tgagaacaat cgccaaagcc agataaatca ggttaacggt ccacatccac agcgtcgggt
    13561 ccagcttacc tttaccgccg ttcgatttca gggaggtctg gatcaggaag aaaagtagat
    13621 acagcagcat ggctggcagc atcgacagta cgcgtccctg acgtgggtta accacgctca
    13681 gcggtacgac cataagtgcc atcataaaca cggtgaatac caacgtgata cgccagttca
    13741 gttctgcgcg agcacgatcg gtgtcagtgt tccacaatgt gcgcatgtcc atctggtcgg
    13801 tatcgttcgg gtcgagcgcc accgcctggt gaccaatgat cgcctgataa tcctggaaat
    13861 ccgtaatgcg gaaatcacgt aacagtgcag tgccttcgaa gcgcgttccc tggttgagag
    13921 tgacgacctg ggagccgtcg cgcagctggg ttaaatgtcc ggaatcggcc accaccacag
    13981 aaggacgtgc attacctttt ggtcgaattt gtgcgaggaa cacatctttg aaatcgctgc
    14041 cgtcaacgct ttcgatgaac agcaccgagc tgccattagt cgcttgctgg aattgccctt
    14101 gcgccagcgc cgccatgcca gggttcgctt tcgcttctgc taacacttca tcctgatgac
    14161 gcgatgacca cggtcccgcc cacatcacgt taaccgccgc gacgattgcc gtgaataccg
    14221 caaggatcat tgccgctttc accagaaccg ctttgctcag gccgcaggca tgcattaccg
    14281 taatttcact ttcggtatac agtttgccca gcgtcatcag cagcccgagg aacaggctta
    14341 atggcagaat aagctgcgcc atttccggca cgcccaaccc gagaagggag agcaccagat
    14401 tcgccggaat atcgccgtca accgctgcgc cgaggatcct cactaacttt tgacagaaga
    14461 agatcaaaag caagatgaag agtatcgcca gctggctttt gagcgtctcc cgcaccagat
    14521 atcttatgat tatcacttta aatacgcccg taaaaactcg tcttttgcag gattttagct
    14581 tgtttcatgg cttaaacgtc atttattctc ttgagtcgtc gaaatcgtcg ctaagataat
    14641 tatactcaac ggattcacct ctcagatttt gttctgacgt gccaatgccg taataacgtt
    14701 aagattaaca cgaagtcatc gcaacagcgg acatgagtta cgaaagcttg caattctatc
    14761 tgtagccacc gccgttgtct ttaagattca ggagcgtagt gcatggagtt tagtgtaaaa
    14821 agcggtagcc cggagaaaca gcggagtgcc tgcatcgtcg tgggcgtctt cgaaccacgt
    14881 cgcctttctc cgattgcaga acagctcgat aaaatcagcg atgggtacat cagcgccctg
    14941 ctacgtcggg gcgaactgga aggaaaaccg gggcagacat tgttgctgca ccatgtacca
    15001 aacgtgcttt ctgaacgcat cctccttatt ggttgcggca aagaacgtga gctggatgag
    15061 cgtcagtaca agcaggttat tcagaaaacc attaatacgc tgaatgatac tggctcaatg
    15121 gaagcggtct gctttctgac tgaactgcac gttaaaggcc gtaacaacta ctggaaagtg
    15181 cgtcaggctg tcgagacggc aaaagagacg ctctacagtt tcgatcagct gaaaacgaac
    15241 aagagcgaac cgcgtcgtcc gctgcgtaaa atggtgttca acgtgccgac ccgccgtgaa
    15301 ctgaccagcg gtgagcgcgc gatccagcac ggtctggcga ttgccgccgg gattaaagca
    15361 gcaaaggatc ttggcaatat gccgccgaat atctgtaacg ccgcttacct cgcttcacaa
    15421 gcgcgccagc tggctgacag ctacagcaag aatgttatca cccgcgttat cggcgaacag
    15481 cagatgaaag agctggggat gcattcctat ctggcggtcg gtcagggttc gcaaaacgaa
    15541 tcgctgatgt cggtgattga gtacaaaggc aacgcgtcgg aagatgcacg cccaatcgtg
    15601 ctggtgggta aaggtttaac cttcgactcc ggcggtatct cgatcaagcc ttcagaaggc
    15661 atggatgaga tgaagtacga tatgtgcggt gcggcagcgg tttacggcgt gatgcggatg
    15721 gtcgcggagc tacaactgcc gattaacgtt atcggcgtgt tggcaggctg cgaaaacatg
    15781 cctggcggac gagcctatcg tccgggcgat gtattaacca ccatgtccgg tcaaaccgtt
    15841 gaagtgctga acaccgacgc tgaaggccgc ctggtactgt gcgacgtgtt aacttacgtt
    15901 gagcgttttg agccggaagc ggtgattgac gtggcgacgc tgaccggtgc ctgcgtgatc
    15961 gcgctgggtc atcatattac tggtctgatg gcgaaccata atccgctggc ccatgaactg
    16021 attgccgcgt ctgaacaatc cggtgaccgc gcatggcgct taccgctggg tgacgagtat
    16081 caggaacaac tggagtccaa ttttgccgat atggcgaaca ttggcggtcg tcctggtggg
    16141 gcgattaccg caggttgctt cctgtcacgc tttacccgta agtacaactg ggcgcacctg
    16201 gatatcgccg gtaccgcctg gcgttctggt aaagcaaaag gcgccaccgg tcgtccggta
    16261 gcgttgctgg cacagttcct gttaaaccgc gctgggttta acggcgaaga gtaattgcgt
    16321 caggcaaggc tgttattgcc ggatgcggcg tgaacgcctt atccgaccta cacagcactg
    16381 aactcgtagg cctgataaga cacaacagcg tcgcatcagg caatgctgtt attgtcggat
    16441 gcggcatgaa cgccttatcc gacctacaca gcaccgaact cgtaagcctg ataagacgcg
    16501 gcagcgtcgc atcaggcaat gctgttattg tcggatgcgg cgtgagcgcc ttatccgacc
    16561 tacacacagc actgaactcg taggcctgat aagacacaac agcgtcgcat caggcgctgc
    16621 ggtgtatacc tgatgcgtat ttaaatccac cacaagaagc cccatttatg aaaaacgcga
    16681 cgttctacct tctggacaat gacaccaccg tcgatggctt aagcgccgtt gagcaactgg
    16741 tgtgtgaaat tgccgcagaa cgttggcgca gcggtaagcg cgtgctcatc gcctgtgaag
    16801 atgaaaagca ggcttaccgg ctggatgaag ccctgtgggc gcgtccggca gaaagctttg
    16861 ttccgcataa tttagcggga gaaggaccgc gcggcggtgc gccggtggag atcgcctggc
    16921 cgcaaaaacg cagcagcagc ccgcgcgata tattgattag tctgcgaaca agctttgcag
    16981 attttgccac cgctttcaca gaagtggtag acttcgttcc ttatgaagat tctctgaaac
    17041 aactggcgcg cgaacgctat aaagcctacc gcgtggctgg tttcaacctg aatacggcaa
    17101 cctggaaata atggaaaaga catacaaccc acaagatatc gaacagccgc tttacgagca
    17161 ctgggaaaag cagggctact ttaagcctaa tggcgatgaa agccaggaaa gtttctgcat
    17221 catgatcccg ccgccgaacg tcaccggcag tttgcatatg ggtcacgcct tccagcaaac
    17281 catcatggat accatgatcc gctatcagcg catgcagggc aaaaacaccc tgtggcaggt
    17341 cggtactgac cacgccggga tcgctaccca gatggtcgtt gagcgcaaga ttgccgcaga
    17401 agaaggtaaa acccgtcacg actacggccg cgaagctttc atcgacaaaa tttgggaatg
    17461 gaaagcggaa tccggcggca cgattacccg tcagatgcgc cgtctcggca actccgtcga
    17521 ctgggagcgt gaacgcttca ccatggacga aggcctgtcc aatgcggtga aagaagtttt
    17581 cgttcgtctg tataaagaag acctgattta ccgtggcaaa cgcctggtaa actgggatcc
    17641 gaaactgcgc accgctatct ctgacctgga agtcgaaaac cgcgaatcga aaggttcgat
    17701 gtggcacatc cgctatccgc tggctgacgg tgcgaaaacc gcagacggta aagattatct
    17761 ggtggtcgcg actacccgtc cagaaaccct gctgggcgat actggcgtag ccgttaaccc
    17821 ggaagatccg cgttacaaag atctgattgg caaatatgtc attctgccgc tggttaaccg
    17881 tcgtattccg atcgttggcg acgaacacgc cgacatggaa aaaggcaccg gctgcgtgaa
    17941 aatcactccg gcgcacgact ttaacgacta tgaagtgggt aaacgtcacg ccctgccgat
    18001 gatcaacatc ctgacctttg acggcgatat ccgtgaaagc gcccaggtgt tcgataccaa
    18061 aggtaacgaa tctgacgttt attccagcga aatccctgca gagttccaga aactggagcg
    18121 ttttgctgca cgtaaagcag tcgttgccgc agttgacgcg cttggcctgc tggaagaaat
    18181 taaaccgcac gacctgaccg ttccttacgg cgaccgtggc ggcgtagtta tcgaaccaat
    18241 gctgaccgac cagtggtacg tgcgtgccga tgtcctggcg aaaccggcgg ttgaagcggt
    18301 tgagaacggc gacattcagt tcgtaccgaa gcagtacgaa aacatgtact tctcctggat
    18361 gcgcgatatt caggactggt gtatctctcg tcagttgtgg tggggtcacc gtatcccggc
    18421 atggtatgac gaagcgggta acgtttatgt tggccgcaac gaagacgaag tgcgtaaaga
    18481 aaataacctc ggtgctgatg ttgtcctgcg tcaggacgaa gacgttctcg atacctggtt
    18541 ctcttctgcg ctgtggacct tctctaccct tggctggccg gaaaataccg acgccctgcg
    18601 tcagttccac ccaaccagcg tgatggtatc tggtttcgac atcattttct tctggattgc
    18661 ccgcatgatc atgatgacca tgcacttcat caaagatgaa aatggcaaac cgcaggtgcc
    18721 gttccacacc gtttacatga ccggcctgat tcgtgatgac gaaggccaga agatgtccaa
    18781 atccaagggt aacgttatcg acccactgga tatggttgac ggtatttcgc tgccagaact
    18841 gctggaaaaa cgtaccggca atatgatgca gccgcagctg gcggacaaaa tccgtaagcg
    18901 caccgagaag cagttcccga acggtattga gccgcacggt actgacgcgc tgcgcttcac
    18961 cctggcggcg ctggcgtcta ccggtcgtga catcaactgg gatatgaagc gtctggaagg
    19021 ttaccgtaac ttctgtaaca agctgtggaa cgccagccgc tttgtgctga tgaacacaga
    19081 aggtcaggat tgcggcttca acggcggcga aatgacgctg tcgctggcgg accgctggat
    19141 tctggcggag ttcaaccaga ccatcaaagc gtaccgcgaa gcgctggaca gcttccgctt
    19201 cgatatcgcc gcaggcattc tgtatgagtt cacctggaac cagttctgtg actggtatct
    19261 cgagctgacc aagccggtaa tgaacggtgg caccgaagca gaactgcgcg gtactcgcca
    19321 tacgctggtg actgtactgg aaggtctgct gcgcctcgcg catccgatca ttccgttcat
    19381 caccgaaacc atctggcagc gtgtgaaagt actttgcggt atcactgccg acaccatcat
    19441 gctgcagccg ttcccgcagt acgatgcatc tcaggttgat gaagccgcac tggccgacac
    19501 cgaatggctg aaacaggcga tcgttgcggt acgtaacatc cgtgcagaaa tgaacatcgc
    19561 gccgggcaaa ccgctggagc tgctgctgcg tggttgcagc gcggatgcag aacgtcgcgt
    19621 aaatgaaaac cgtggcttcc tgcaaaccct ggcgcgtctg gaaagtatca ccgtgctgcc
    19681 tgccgatgac aaaggtccgg tttccgttac gaagatcatc gacggtgcag agctgctgat
    19741 cccgatggct ggcctcatca acaaagaaga tgagctggcg cgtctggcga aagaagtggc
    19801 gaagattgaa ggtgaaatca gccgtatcga gaacaaactg acgaacgaag gctttgtcgc
    19861 ccgcgcaccg gaagcggtca tcgcgaaaga gcgtgagaag ctggaaggct atgcggaagc
    19921 gaaagcgaaa ctgattgaac agcaggctgt tatcgccgcg ctgtaatcaa aacacagtga
    19981 tgaaaacgaa ggccggagca tgctccggcc ttttttatct cttacagaaa tggtaaagaa
    20041 ggcataattc cccgtgaaat actggctaag aagcctttat ctggttgttc ttctttattg
    20101 attaaaggaa ggtcatccaa atcgcccagc aaatacgtat tttttgcctg ccagtcaatc
    20161 atccatattt tcagcagtgg ataagccaga ccgcccgtaa tcccggatat caccaccaac
    20221 gcacacatgc gataaagcat accgtggtat gttaaagttg agcgaaaacg aatacgccca
    20281 tcattcagtg acaggttgct cataaaatga tttcgcaacg acaccgttaa ataactggtg
    20341 ctaacagcaa tcccaaaata atagattaac tgcgcgatta tcattttacg ttgcatttcc
    20401 ataaattgct gtaaattctc aatatcatca tttgcataca cagaactatc atacgcattt
    20461 aatatttgat cgaagataat ataaccagca acagcgagaa aaggtaataa agctaaaaat
    20521 gcgagaatgg catattttat acaatacgta gtatctaatt ttaccttgaa acgatgtata
    20581 ccgaaactgg tattgctcca gagaaaactc attaccagac tatataaagt accattaaaa
    20641 ataccaatgg aaacaatacc aactattgcc atcagaacca cagatataat aacactactt
    20701 gaactattgg catgtagcat ctttgtcgag ataaagaaaa cagtccccat cccaatggcc
    20761 attaaaatcg gcaagaaaaa ggttacccac cagaaccctt tcaaagagca attaaaacta
    20821 aatcttacgc cgttgagact agtcatcaag gcctgataac gcagtccttt tgctgccata
    20881 aatataagca aaaccaacag tgacaaagtc aaaacacagc caacaagcgg catatctgct
    20941 gacactgtca ttaagattgc gaaatagaaa aaaacaaaaa taagacaact aacaaaaaca
    21001 ttcccaccgg taatcccata agaaaatcgt tgtccgttaa cttccatatt agcatagaga
    21061 taacgcttac atttcattaa tgcccatggt aaatagatac ctagcgttat aatcgttaac
    21121 aacacattca ccacacaaat aagaaaatat ctctcacctg taccatgaaa aaca
//
LOCUS       DACBVI010000073        21105 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.073, whole genome shotgun sequence.
ACCESSION   DACBVI010000073 DACBVI010000000
VERSION     DACBVI010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.073"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            329..1744
                     /gene="gltX"
                     /locus_tag="IU325_13145"
     CDS             329..1744
                     /gene="gltX"
                     /locus_tag="IU325_13145"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP5809963.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            complement(1795..2166)
                     /locus_tag="IU325_13150"
     CDS             complement(1795..2166)
                     /locus_tag="IU325_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAP5809964.1"
                     /translation="MTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGGRARLILV
                     DTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTHSEQEKVA
                     QFLSREGIRNFLARLDIDESE"
     gene            complement(2189..2533)
                     /locus_tag="IU325_13155"
     CDS             complement(2189..2533)
                     /locus_tag="IU325_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAP5809965.1"
                     /translation="MTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKARLVHV
                     NEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLLREGII
                     GLLQRLGIRDSK"
     gene            2769..2844
                     /locus_tag="IU325_13160"
     tRNA            2769..2844
                     /locus_tag="IU325_13160"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2802..2804,aa:Ala,seq:ggc)
     gene            2884..2959
                     /locus_tag="IU325_13165"
     tRNA            2884..2959
                     /locus_tag="IU325_13165"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2917..2919,aa:Ala,seq:ggc)
     gene            3167..5356
                     /locus_tag="IU325_13170"
     CDS             3167..5356
                     /locus_tag="IU325_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAP5809966.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLSKEKRAFTLS
                     WLAALSVLLLLMCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVLTEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYNISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(5407..6609)
                     /gene="nupC"
                     /locus_tag="IU325_13175"
     CDS             complement(5407..6609)
                     /gene="nupC"
                     /locus_tag="IU325_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HAP5809967.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            6945..8183
                     /gene="mntH"
                     /locus_tag="IU325_13180"
     CDS             6945..8183
                     /gene="mntH"
                     /locus_tag="IU325_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+) uptake NRAMP transporter MntH"
                     /protein_id="HAP5809968.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            complement(8325..8651)
                     /gene="ypeC"
                     /locus_tag="IU325_13185"
     CDS             complement(8325..8651)
                     /gene="ypeC"
                     /locus_tag="IU325_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HAP5809969.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(8766..10022)
                     /locus_tag="IU325_13190"
     CDS             complement(8766..10022)
                     /locus_tag="IU325_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HAP5809970.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPFWIIAILTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVMGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            10226..11191
                     /locus_tag="IU325_13195"
     CDS             10226..11191
                     /locus_tag="IU325_13195"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HAP5809971.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWISLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            11410..11736
                     /locus_tag="IU325_13200"
     CDS             11410..11736
                     /locus_tag="IU325_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HAP5809972.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            11758..13005
                     /locus_tag="IU325_13205"
     CDS             11758..13005
                     /locus_tag="IU325_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP5809973.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            13020..14105
                     /locus_tag="IU325_13210"
     CDS             13020..14105
                     /locus_tag="IU325_13210"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP5809974.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     VSAHILVDSRYYADVEARTQGYQLHLLDATNTLTTIVNQIIADEQLQALGFEGQQVCW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVTAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRV
                     ITEAGYGDYFGHNTGHAIGIEVHEEPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            14105..15142
                     /locus_tag="IU325_13215"
     CDS             14105..15142
                     /locus_tag="IU325_13215"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP5809975.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQSVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIQRLT
                     RETVVQLTDFR"
     gene            15167..17662
                     /gene="ptsP"
                     /locus_tag="IU325_13220"
     CDS             15167..17662
                     /gene="ptsP"
                     /locus_tag="IU325_13220"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP5809976.1"
                     /translation="MLTIQFLCPLPNGIHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMAEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMERDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIITTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQSFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(17665..18522)
                     /locus_tag="IU325_13225"
     CDS             complement(17665..18522)
                     /locus_tag="IU325_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP5809977.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRSDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(18535..19269)
                     /locus_tag="IU325_13230"
     CDS             complement(18535..19269)
                     /locus_tag="IU325_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP5809978.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(19284..20981)
                     /locus_tag="IU325_13235"
     CDS             complement(19284..20981)
                     /locus_tag="IU325_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP5809979.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
BASE COUNT         5015 a         5271 c         5633 g         5186 t
ORIGIN      
        1 gtgatgacgg gatcgaaccg ccgaccccct ccttgtaagg gaggtgctct cccagctgag
       61 ctaatcaccc ccgctgtgtg gagtcgcatt atagggagag ttcaaaatga gtcaacgcat
      121 tttctaaaga aattgttcgt tcgtcgtaaa tttaagcaag atgatcgcaa aacagaccgt
      181 gttgcgcaat ttgtcaacga aaacaataat gcgtaaggtt gcaacccgaa ctacattgag
      241 gaatcaggcg ggagtgatag aatatcgccc actcaatttt tccaggattt gccggttgtc
      301 ggcatctttc taaacgtaag gccatttcat gaaaatcaaa actcgcttcg cgccaagccc
      361 aacaggctat ctgcacgttg gcggcgcgcg tactgctctt tactcctggc tttttgcacg
      421 taaccacggc ggtgagttcg tgctgcgtat tgaagacacc gatcttgagc gttccacgcc
      481 ggaagctatc gaagccatta tggatggcat gaactggctg agcctggagt gggatgaagg
      541 tccgtactac cagaccaaac gttttgatcg ctacaacgcg gtgatcgatc agatgctgga
      601 agagggcact gcttataaat gctattgctc taaagagcgc ctggaagcgc tgcgcgaaga
      661 gcaaatggcg aaaggtgaga agccgcgtta tgacggtcgc tgccgccaca gccatgagca
      721 tcatgctgat gatgaaccgt gtgttgtacg ttttgctaac ccgcaggaag gttctgttgt
      781 ttttgacgat cagatccgtg gtccgatcga attcagcaac caggagctgg atgatctgat
      841 tatccgtcgt accgatggtt ctccaaccta taacttctgt gtggttgtcg atgactggga
      901 tatggaaatc acccacgtta tccgtggcga agaccatatc aacaacacgc cacgccagat
      961 caacattctt aaagcactga aagcgccggt accggtttac gcgcacgttt ctatgatcaa
     1021 cggcgatgac ggtaaaaaac tgtccaaacg tcacggggcg gtcagcgtaa tgcagtatcg
     1081 tgatgatggt tatctgccag aagcactgct gaactatctg gtgcgtctgg gctggtccca
     1141 cggcgatcag gaaatcttca ctcgtgaaga gatgatcaaa tacttcaccc tgaatgccgt
     1201 cagcaaatct gccagtgcgt tcaacaccga caagctgcta tggctgaacc atcactacat
     1261 taacgcgcta ccgccggagt atgttgctac tcacttacag tggcacattg agcaggaaaa
     1321 tatcgatacc cgtaacggcc cgcagctggc tgatctggtg aaactactgg gtgaacgctg
     1381 caagacgctg aaagagatgg cacagagctg ccgttatttc tacgaagatt ttgctgagtt
     1441 cgatgccgac gccgcgaaaa aacatctgcg tccggtagcg cgtcagccgc tggaagtggt
     1501 tcgtgacaaa ctggccgcga ttactgactg gaccgctgaa aacgttcatc acgctattca
     1561 ggcgacggcg gatgagctgg aagtgggtat gggtaaagtt ggtatgccgc tgcgtgtcgc
     1621 cgtaaccggt gcagggcagt ctccggcgct ggatgttacc gttcacgcaa tcggtaagac
     1681 ccgcagtatc gagcgtatca acaaagcgct ggcttttatt gctgaacgcg aaaatcagca
     1741 gtaattagcg cataaaagat aaacggcagg agagataccc ctgccgtttt ttatttattc
     1801 tgattcatct atgtcgagac gagcaaggaa attacgaatt ccttcgcgtg acaaaaattg
     1861 cgcaactttt tcctgctcac tgtgagtcat gttgtctatt gcgctgatga tttgccgata
     1921 agcaggggtg cgaatacccg gtgcgtattc ggcaaggggt tcttcggctt ccagaagcat
     1981 cggtgtgtta tggaaggctg gcgtgttctg aataaactcg caaacttgcg tatcgaccag
     2041 aataagtctg gcacgaccgc ctttcacgcc aggaaatttt tccgttttcc agcctttttc
     2101 tctgatccaa cggttaacag tttgtttagc cacaccgagg cattcagcca gttcttcggt
     2161 ggtcatttta ttgcgtaatc ttttcatatt atttgctatc gcgaatccct aagcgttgta
     2221 ataatccgat aatcccttcc cgcagaagca aggatgtaaa ctgtttttgt tcaactggcg
     2281 tcatctcttt cgccagtgtc accagcaaca cttcaagcgg cgcatcacca gacagggcag
     2341 gcgcttctcc ctgaccttct ggacgttcag cattgcgaat atattcacga acctgttcat
     2401 tgacgtgaac cagtcgggct ttgccaccct ggacgccagg ttttggtgac gttgtccagc
     2461 cttccttgcg tacccattta ttaatggtct gccggctata gccggtcagt ctggcaagtt
     2521 catctggcgt catcctctcc ttgaacataa caattccctg aatgtatgtg ggtctattag
     2581 tagttcattt tataacacca ctttacagga aaccgtgaca tgaataacta ctgaatgcga
     2641 gcggttttcc aaaattgttc atttgcctga tttttcagcg attgaaacta cacgttgcaa
     2701 tttgccgttg acacattcgg gcggaattca tatgatgccg cccgtcaact cgacaagctt
     2761 tacgtgacgg ggctatagct cagctgggag agcgcttgca tggcatgcaa gaggtcagcg
     2821 gttcgatccc gcttagctcc accaaatttt gcacccagca aatttggtac gtaaacgcat
     2881 cgtggggcta tagctcagct gggagagcgc ttgcatggca tgcaagaggt cagcggttcg
     2941 atcccgctta gctccaccaa atttcaaacc ctcgctgtaa agcgggggtt ttttgtctct
     3001 gctttttgcc gcttttgtaa tacagtctac gtccgggtta gcgccgcctg gtgaaagcat
     3061 cattggatga aaaatcggca acaggctggc ccctgtttgc ttcgcgatgc gaataaactt
     3121 attatttgtg tgcctgaaaa ccccgatcag tgagagtagt gtactcatgt ttgtggagca
     3181 taacctgata aaaaatatca agatattcac actagcgttt acgctcaccg tggtacttat
     3241 tcagctatcc cgttttattt cgccacttgc cattatccat tccagttata tctttctggc
     3301 gtggatgcca ctgtgcgtaa tgctgtcaat cttgtttatc tttggctggc gcggtgtcgt
     3361 tcccgtttta tgcgggatgt tttgcaccaa tctgtggaac tttcatctat cttttttaca
     3421 gaccgcggtc atgctcggca gccagacgtt tgttgtgttg tgtgcctgcg caatattacg
     3481 ctggcagctg gggacgcgtt ggcgttatgg attgaccagc cgatatgtct ggcaacgtct
     3541 tttctggctt ggtttggtga cgccgatcgg catcaaatgc agcatgtatc ttgtggggag
     3601 cttttttgat ttcccgctaa agatatccac ctttttcggc gatgcggatg ccattttcac
     3661 agtcgttgat ttactaagcc ttttcaccgc tgtgctgatt tacaacatgc ttttctacta
     3721 tctcactcgc atgattgtaa gtccccactt tgcgcagata ttgtggcgca gggatatcgc
     3781 tccgtcgttg agcaaagaga aacgcgcatt taccttaagc tggctggcag ctcttagcgt
     3841 gctgctactt ctgatgtgca caccgtatga gaatgacttt attgccggtt acctggtacc
     3901 tgttttcttt atcatcttta ccctcggtgt cggtaagctt cgctatccgt ttttaaatct
     3961 cacctgggct gtttcaacgc tttgcctgct gaattacaac cagaactttt tgcaaggggt
     4021 actaaccgaa tattcgctgg catttattct cgcggtgctg atttccttta gtgtttgcct
     4081 gctctatatg gtgcgcattt atcatcgcag tgaatggctt aatcgccgct ggcatttgca
     4141 ggcgctgacc gatccgttaa cgctcctacc caactttcgt gcgttagaac aagcgccgga
     4201 gcaagaggcg ggcaagagtt tttgctgcct gcgcattgat aatcttgagt ttatgagtcg
     4261 tcattacggc ttaatgatgc gcgttcactg tatccgatca atttaccgta cgctgctgcc
     4321 gttgatgcag gaaaacgaaa agttgtatca attgccgggt agtgaactgc tgttagtgct
     4381 gagtggaccg gaaacggaag ggcgactcca gcatatggtt aacatcctga atagtcggca
     4441 aattcactgg aataataccg ggctggatat gggctatggt gctgcctggg ggcgttttga
     4501 tggaaatcag gaaaccctgc aacccttgtt ggggcagtta agctggctgg cggagcaatc
     4561 ctgcgcacat caccatgtgc tggcgctgga tagccgagag gagatggttt ccgggcagac
     4621 cactaaacag gtgctattgc tgaataccat tcgtacggcg ttagatcagg gtgatttgct
     4681 gctctacgcc cagccaattc gcaacaaaga gggtgaaggt tatgatgaaa tcctcgcgcg
     4741 actgaaatat gacggcggca ttatgacccc ggataagttt ctgcccctta ttgctcagtt
     4801 taaccttagc gcgcgttttg atttgcaagt gctggaatcc ttgttgaagt ggctggcaac
     4861 acatccttgc gacaaaaaag gcccgcgctt ttcagtcaat ttaatgccgc tcacgctgct
     4921 gcaaaagaat attgccgggc ggattattcg tctgtttaag cgttataaca tctccccgca
     4981 ggcggtcatt cttgagatca ccgaggagca ggcgttttct aacgcagaaa gcagcatgta
     5041 caacatcgag cagctgcata agtttggttt ccggattgcg attgatgact ttggcaccgg
     5101 ctatgccaac tacgaacggt taaagcgttt gcaggctgat atcatcaaaa ttgatggcgt
     5161 ctttgtgaaa gatattgtca cgaatacgct ggatgcgatg attgtgagat caattaccga
     5221 tctggcgaaa gcgaagtcat tgagtgtggt cgcggagttt gtcgagacgc aacagcagca
     5281 ggcgctattg cataagctcg gggtgcaata tctgcaaggg tatttgattg gtcgcccgca
     5341 gccattagct gattaacagg cgtaaaaaaa accggggaat tatccccggc ttttttatgt
     5401 atggtcttac agcaccagtg ctgcgattga cgcagacagc acactcacca gggtagagcc
     5461 gtaaaccagc ttcagaccga agcgagaaac cacgttacct tgctcttcat tcaggccttt
     5521 aactgcacct gcgataatcc cgattgaaga gaagttagcg aaggaaacca ggaacacaga
     5581 gatgatgcct tcagcacgcg gagagagtgt agaggcaatt ttctgcagat ccatcatcgc
     5641 aacgaactcg ttggaaacca gtttggtcgc catgatactg cccacttgca gtgcttcact
     5701 ggaaggaaca cccatcaccc atgcaatcgg atagaagatg tagcccagga tgccctggaa
     5761 ggagatgctg tagccaaacc agccagtcac ggtggcaaac agtgcgttca gcgcggcgat
     5821 cagggcgata aagccgatca gcatcgcggc aacgataatg gcgactttga aacctgccag
     5881 aatgtattca cccagcattt cgaagaagct ctggccttcg tgcaggttgg acatctggat
     5941 gttttcttca ctggcatcaa cacggtaagg gttgatcagc gacagcacga taaaggtgct
     6001 gaacatgttc agtaccagtg ccgcaacgac gtatttcggt tccagcatgg tcatgtatgc
     6061 accaacgatg gacatcgaca cggtggacat cgccgttgct gccatggtgt acatacgatt
     6121 gcgggagatt ttgccgagga tgtctttata ggcaataaag ttttcagact gacccagaat
     6181 cagggagctg acggcgttaa aggattccag tttgcccatg ccgttgactt tggagagcag
     6241 gaaaccaatt gcgcggataa tcaccggcaa cacgcgaatg tgctggagaa taccgatcaa
     6301 tgcagagata aagacgattg ggcacagcac tttcaggaag aagaatgcca ggccttgatc
     6361 attcatgcta ccaaagacga agttagtccc ttcgttggca aatccgagca gtttttcgaa
     6421 catttcggag aagcctttca cgaagcctaa accaacgtcg gagttcagga agaaccacgc
     6481 cagtaacact tcgataacaa gcagttgaat aacataacgg atacgaattt ttttgcggtc
     6541 gctgcttacc agcagtgcga gaatcgcaac aacggcaagt gccagtacaa aatgaaggac
     6601 gcggtccata tttgctccaa atatgaggca ggttaaattt ccgtgcacat tctatgtaac
     6661 atggttaagg aaaacgagat ctgacacaca ctataatgac gtcctgtgac aagcttcaaa
     6721 aatagtgagc tgtcatacat ttctgctatg ttgtgtatgg aagctgaaag ttatgtaaat
     6781 gtgctaacat taaacataac cataacattc aagtcatcaa tcttttcgta gtcataatcc
     6841 tggtctatca gagaaatcac cacaacccat ttaaatgaat ttgataatca ttctcgtttg
     6901 gcatagcatg aaacatagca aaggctatgt tttagaggca caagatgacg aactatcgcg
     6961 ttgagagtag cagcggacgg gcggcgcgca agatgaggct cgcattaatg ggacctgcgt
     7021 tcattgcggc gattggttat atcgatcccg gtaactttgc gaccaatatt caggcgggtg
     7081 ccagcttcgg ctatcagcta ctgtgggttg tcgtttgggc caacctgatg gcgatgctga
     7141 ttcagatcct ctctgccaaa ctggggattg ccaccggtaa aaatctggcg gagcagattc
     7201 gtgatcacta tccgcgtccc gtagtgtggt tctattgggt tcaggcagaa attattgcga
     7261 tggcaaccga cctggcggaa tttattggtg cggcgatcgg ttttaaactc attcttggtg
     7321 tttcgttgtt gcaaggcgcg gtgctgacgg ggatcgcgac tttcctgatt ttaatgctgc
     7381 aacgtcgcgg gcaaaaacca ctggagaaag tgattggcgg gttactgttg tttgttgccg
     7441 cggcttacat tgtcgagttg attttctccc agcctaacct ggcgcagctg ggtaaaggaa
     7501 tggtgatccc gagtttacct acttcggagg cggtcttcct ggcagcaggc gtgttagggg
     7561 cgacgattat gccgcatgtg atttatttgc actcttcgct cactcaacat ttacatggcg
     7621 gttctcgtca acaacgttat tccgccacca aatgggatgt ggctatcgcc atgactattg
     7681 ccggttttgt caatctggcg atgatggcta cagctgcggc ggcgttccat ttttcaggtc
     7741 atactggtgt tgccgatctt gatgaggctt acctgacgct gcaaccgttg ttaagccatg
     7801 ctgcggcaac ggtctttgga ttaagcctgg ttgctgccgg actgtcctca acggtggtgg
     7861 ggacactggc ggggcaggtg gtgatgcagg gcttcattcg ctttcatatc ccgctgtggg
     7921 tgcgtcgtac agtcaccatg ctgccgtcat ttattgtcat tctgatggga ttagatccga
     7981 cacggattct ggttatgagt caggtgctgt taagttttgg tatcgctctg gcgctggttc
     8041 cactgctgat ttttaccagt gacagtaagt tgatgggcga tctggtgaac agtaaacgcg
     8101 taaaacagac aggctgggtg attgtggtgc tggtagtggc gctgaatatc tggttgttgg
     8161 tggggacggc gctggggcta tagttcaact ggcgtcgtac cagtcatcac tggcggatgc
     8221 ggcgtaaaac gccttatccg ccctacgcgt taaagagacg gtttgtaggc ctgacaagac
     8281 gcgctagcgt cgcatcaggc gttgtgctcc attggcgaat gtgtttaatg gcggtgtcca
     8341 tgtccgcggc cttttccgcg atgatcgtca cgatcgcgcc agccttcacg atagccacgc
     8401 tcatacgctt tacgcttatc ccagccacga tggtagccat tatcatgacg ccaccagcgg
     8461 ttcttacgcc attcataatt gcgatgccag tagtcacggt cgcgccagtg accaccgtcc
     8521 cagtaattac cgtaatgatc gcgatcgcca atttgtaatt tgattgatgg cagtagggtg
     8581 atttcacctg cgtttgccgc aagcggggta aatgccatca gggcggccgc cagaaacagt
     8641 gacctgaaca ttgttattct ccttcacgat cgaagccgtc agcggcctgt taacgcaata
     8701 ttacggggag gtaaagcccc gtatcatcgc cataactctt aaatcataaa ggagagcatt
     8761 ttttgctaaa cgttatcgtg gggtggctgt tgcttcggac gattgaccat catcatcggc
     8821 ttacctgcta ataacagcca tgccggaagc atgacgatac agagcagcgg tagcaatgtg
     8881 gtattgggta caacgactgc cgccataaaa agacttaacc agccatcgcg tgttaccacc
     8941 agcacgatgc cgagaatagc gcaggaaacg gttattgccg ctggaacggc ggggacgtgc
     9001 tcatgcagca tcaaccctaa tgccacgccg acaaacactg ccgggaagat tcgcccaccg
     9061 cgaaagccac tggcggcagc aacgaccagg gcggcaagtt taattaccgc cagcaaaaag
     9121 taatcgctgg tgctgaaagc ctgatttgcc accatctgct gcatctcatc cagcccttta
     9181 aacagagaaa ctggtccacc cataaccccc agaataccga gaataaatcc gccaataccc
     9241 agcacgagca ccggattttt catttgatgc atcatcgcgt gcaaccgtgg taagcaccat
     9301 acggcgacca tccctgctgc gatggcaatc gccgcgacaa ttgcaccgct gagaatatcg
     9361 gtcatttcca tttgtccgta atgcgcaatg ggcagtgaaa aatggggatg gaaaaataat
     9421 ccggtagtaa gtgcaccagc tgctgccgcc attaacggcg caaaaagacg atcccatagc
     9481 ggaacttcac tactaccatt taaggtttgc gaaaatatca acgccgccgc aacaggtgtg
     9541 ccaaacagcg cgccgatggt tccggcagag gctaaaatag tccactccat tcggttgaca
     9601 cgcggtaaca gacgagcgcc aatggcaacc gcaagggcga tattgacggt catgatcgga
     9661 tgttctggcc ccaggctgac gccaccagca agaccaagaa ttaatgctac gataagtcct
     9721 ggtagcgcag agggcggaac tggtgcgccg atcagcggtt cacaggcggg gtctggtccg
     9781 gcatgcccct ggctgaaacg gataaccaac cccaccgcaa tacctgttag cgttaatatg
     9841 gcgatgatcc agaaaggtga atcctgggct atccccagag ttcccggcag tcgttgccag
     9901 agcaaattct gtaataccga ggcgattttc atcaccacaa tcagaataag actggacgca
     9961 atcccaattg ccacggcggg gagcgataat aacaacatgg ttctggctcg cggatggagc
    10021 atattctatt ccttatgcgg ggtcagatac ttagtttgcc cagcttgcaa aaaggcatcg
    10081 ctgcaattgg tgctgaaacg ataaagtaat tgtgtgaccc agatcgatat ttacagggag
    10141 cctgcctttc cggcgttgtt gttatgcccc caggtattta cagtgtgaga aagaattatt
    10201 ttgactttag cggagcagtt gaagaatgac aaagtatgca ttagtcggtg atgtgggcgg
    10261 caccaacgca cgtcttgctc tgtgtgatat tgccagtggt gaaatctcgc aggctaagac
    10321 ctattcaggg cttgattacc ccagcctcga agcggtcatt cgcgtttatc ttgaagaaca
    10381 taaggtcgag gtgaaagacg gctgtattgc catcgcttgc ccaattaccg gtgactgggt
    10441 ggcaatgacc aaccatacct gggcgttctc aattgccgaa atgaaaaaga atctcggttt
    10501 tagccatctg gaaattatta acgattttac cgctgtatcg atggcgatcc cgatgctgaa
    10561 aaaagagcat ctgattcagt ttggtggcgc agaaccggta gaaggtaagc ctattgcggt
    10621 ttacggtgcc ggaacggggc taggggttgc gcatctggtc catgtcgata agcgttggat
    10681 aagcttgcca ggcgaaggcg gtcacgttga ttttgcgccg aatagtgaag aagaggccat
    10741 tatcctcgaa atattgcgtg cggaaattgg ccatgtttcg gcggaacgtg tgctttctgg
    10801 ccctgggctg gtgaatttgt atcgcgcaat tgtgaaagcc gacaaccgcc tgccagaaaa
    10861 tctcaagcca aaagatatta ccgaacgcgc gctggctgac agctgcaccg attgccgccg
    10921 cgcgttgtcg ctgttttgcg tcattatggg ccgttttggc ggcaatctgg cgctcaatct
    10981 cgggacattt ggcggcgtgt ttattgcggg cggtatcgtg ccgcgcttcc ttgagttctt
    11041 caaagcctcc ggtttccgtg ccgcatttga agataaaggg cgctttaaag aatatgtcca
    11101 tgatattccg gtgtatctca tcgtccatga caatccgggc cttctcggtt ccggcgcaca
    11161 tttacgccag accttaggcc acattctgta actccttcct gttatatcgg gaggtaactc
    11221 tcccgataat cttttaaatc atacagttaa ttcaattttc ctttgtgtcc cctcacaagg
    11281 ccgacctgcg tcacacttcc gtacagcggg attaattctc cagtaaatgc attatttgtc
    11341 tggtaacggc gatttgtttt gcacgttcat aatttcactc gtcaactatc actgaacgag
    11401 gaattaacga tgagtaagaa actgattgct ttatgtgcct gcccgatggg tctggctcac
    11461 acctttatgg cagcccaggc gctggaagaa gcggcggtgg aagcaggcta tgaagtgaaa
    11521 attgaaactc agggcgcgga cggcatccag aatcgcctga ccgcgcagga tatcgccgaa
    11581 gcgaccatca tcatccactc cgtggcggtt accccggaag ataacgaacg tttcgaatca
    11641 cgcgacgttt atgaaatcac tttgcaggac gcaattaaaa acgctgcggg catcatcaaa
    11701 gaaatcgaag agatgattgc ctctgaacaa cagtaatatt cgacagggat aattgttatg
    11761 gccattaaaa aacgcagtgc aaccgttgtg cctggcgcat ccggtgcggc agcggcagtt
    11821 aagaatccgc aggcctctaa aagcagcttc tggggtgaac tcccgcagca tgtgatgtca
    11881 gggatttcac gcatggtgcc gaccttaatt atgggcggtg tgatcctcgc tttcagccag
    11941 ctgattgctt atagctggct taaaattcct gctgatatcg gcatcatgga tgctcttaat
    12001 agcgggaaat tctccggttt cgacctttcc ttactgaaat ttgcctggct gtcgcagtcc
    12061 ttcggcggcg tgttgtttgg tttcgccatt ccgatgtttg ccgcttttgt ggcgaactct
    12121 atcggcggca aactggcgtt cccggctggt tttattggcg gcttgatgtc tacccagccg
    12181 acgcaactgc tgaacttcga tcccagcact atgcaatggg cgacctcttc gccggtgccg
    12241 tccaccttca ttggtgcgct gattatttct atcgttgccg gttaccttgt gaaatggatg
    12301 aaccagaaaa tccagttacc ggatttcctg ttagcgttca aaaccacttt tttgctaccg
    12361 attctttccg ccatttttgt catgctggcg atgtactacg tcatcacccc ctttggcggc
    12421 tggatcaacg gcggtatccg taccgtgctg actgccgcag gtgagaaggg cgcgctaatg
    12481 tatgcgatgg ggatcgccgc ggcgacagca atcgaccttg gtggcccgat caacaaagcc
    12541 gcaggtttcg ttgccttcag ctttaccacg gaccacgtat taccggtcac cgcacgttct
    12601 atcgctatcg ttattccgcc gattggtctg ggtctggcga ccattattga ccgtcgttta
    12661 accggcaaac gcctgttcaa cgctcagctt tatccgcagg gtaaaaccgc catgttcctt
    12721 gcctttatgg ggatcagtga aggtgcgatt ccgtttgcgc tggaaagccc catcaccgcc
    12781 attccgtcgt atatggtcgg cgcgattgtc ggctcaaccg ccgctgtctg gctgggcgca
    12841 gtgcaatggt tcccggaatc cgctatctgg gcatggccac tggtcactaa cctgggcgtc
    12901 tatatggcgg ggatcgcgct gggggcagtc attactgcgc tgatggtggt gttcctgcgt
    12961 ctgatgatgt tccgcaaagg taaattgtta atcgatagcc tgtaagaagg acaatcctga
    13021 tgacattact cgcttcgctg cgcgactggc ttaaggcgca acaactggat gcggtgcttc
    13081 tctcctcacg gcagaacaaa cagccgcatc tggggatctc caccggatca ggctatgtgg
    13141 tgattagccg tgtaagtgcg cacattctgg tggattcgcg ctactacgcg gatgtcgaag
    13201 cccgcacgca aggctaccag ctgcatttgc ttgacgcgac gaacacgctt accactatcg
    13261 tcaatcaaat cattgccgat gagcagttgc aagcgctcgg ttttgaaggc cagcaggtat
    13321 gttgggaaac cgcgcaccgc tggcagtctg aactcaatgc gaaactggtt agcgccacgc
    13381 cggatgtgct gcggcaaatc aaaacgccag aggaggtgga gaaaatccgc ctcgcctgtg
    13441 ggattgccga tcgcggtgct gagcatattc gccgctttat tcaggcgggg atgagcgagc
    13501 gcgagatagc cgctgaactg gagtggttta tgcgccagca gggcgcagaa aaagcctctt
    13561 tcgataccat tgtcgccagc ggctggcgtg gggcgctgcc gcacggcaaa gccagcgaca
    13621 agattgttac agcgggcgag tttgtcactc tcgatttcgg tgcgctgtat cagggctact
    13681 gctctgatat gacgcgcacc ttgctggtga atggcgaagg ggtgagcgcc gaatctcacc
    13741 cgctgtttaa cgtctatcaa attgtcctgc aggcacagct cgcagcaatc tccgcgattc
    13801 gccccggcgt gcgctgccag caggttgacg acgccgcgcg ccgggtcatt acagaagcag
    13861 gttatggcga ctatttcggt cataacaccg gtcatgctat cggcattgaa gtccatgaag
    13921 agccgcgttt ttcaccgcgg gataccacga cgctacagcc aggcatgtta ctgaccgtgg
    13981 agccggggat ttatttgcca gggcaagggg gcgtgcgcat cgaagatgtt gtgctggtca
    14041 ccccgcaagg cgcagaagtg ctctacgcca tgccgaaaac agtgttgctc acgggagagg
    14101 cataatggat ttatcgttat taaaagcgtt gagcgaggca gatgcgatcg cctcctcgga
    14161 acaggaagtg cggcagatcc tgctggaaga agcggatcgc ctgcaaaaag aagtgcgatt
    14221 tgacggcctg ggatcggtgc tgatccgcct caatgaatcg acaggtccga aggtgatgat
    14281 ctgtgcgcat atggatgaag tgggatttat ggtgcgcagc atctcccgcg aaggggcgat
    14341 cgatgtgttg ccggttggca atgtacgcat ggctgcccgc cagcttcagt cggtgcgtat
    14401 cactacccgt gaagagtgca aaattccagg cctgcttgac ggcgaccggc aggggaatga
    14461 cgtcagcgcc atgcgcgtgg acattggtgc gcgctcgtat gacgaagtga tgcaggcggg
    14521 aattcgtcca ggcgatcgcg tcacctttga tacaactttt caggttctcc ctcaccagcg
    14581 ggtgatgggg aaagcctttg atgatcgcct cggttgctat ctgctggtga cgttactacg
    14641 tgagctgcac gacgccgaac tgcctgcgga ggtgtggctg gtggccagct ccagcgaaga
    14701 ggtgggatta cgcggcgggc aaaccgctac ccgtgcggtg tcgccggacg ttgcgattgt
    14761 gctggatacc gcctgctggg cgaaaaactt tgattatggc gcggctaacc atcgccagat
    14821 tggtaacggc ccgatgctgg tattaagcga caagtcgctg attgcgccgc caaaacttac
    14881 cgcgtggatt gaaaccgtgg cggcagaaat tggcgtgccg ttacaggcag atatgttcag
    14941 caacggtggc acggacggtg gggcggtgca cttaaccggc actggcgtac ccacagtggt
    15001 gatggggcct gccacccgcc acggacattg cgcggcatcg attgccgatt gtcgcgacat
    15061 tttgcagatg cagcaacttt tatctgccct tattcaacgt cttacgcgtg agacggttgt
    15121 tcaactgacg gatttcagat gatctcctga ttaacccgga gcggttatgc taacgattca
    15181 atttctctgt cctctgccta acggtattca tgctcgtccg gcgtgggaac ttaaagaaca
    15241 gtgcagccag tggcaaagcg aaatcacctt catcaaccat cgccagaacg caaaggccga
    15301 tgcgaaaagt tcgctggcgc tgattggcac cggcacttta tttaatgaca gttgcagcct
    15361 gaacattagc ggcagcgatg aagagcaggc gcggcgcgtg ctggaagagt acatccaggt
    15421 gcgctttatc gacagcgaca gcgtccagcc aacgcaggca gaactgacgg cgcatccgct
    15481 gccgcgttca ttaagccgtc tgaatcctga tttattgtac ggcaatgtgc tggcaagcgg
    15541 cgtcggcgtg ggtacgctga ccctgttaca gagcgacagc ctcgacagtt atcgggccat
    15601 cccggccagt gcgcaagact ccacccggct ggagcacagc ctggcaacgc ttgccgagca
    15661 actgaaccag cagctgcgtg agcgtgacgg cgaaagcaaa actatcctca gcgcccattt
    15721 gtcgctgatt caggatgatg aatttgcagg caatatccgt cgccttatgg cagaacagca
    15781 tcaggggctg ggggcggcga tcatcagcaa tatggagcag gtttgcgcca aactatctgc
    15841 ttctgccagc gattatctgc gcgagcgggt cagcgacatt cgcgatatca gcgaacagtt
    15901 gctgcatatc acctggccgg aactgaagcc gcgcaacaat ctggtgctgg aaaaaccgac
    15961 tattctggtg gccgaggatt taaccccaag ccagtttttg agcctcgatt taaaaaatct
    16021 tgcgggcatg attctggaga aaaccgggcg cacctcgcat accctgattc tggcccgtgc
    16081 ctcggcgatc ccggttctta gcggcctgcc gctggatgcg atcgcgcgct atgccgggca
    16141 accggcggtg cttgacgccc agtgcggtgt gctggcgatt aacccgaatg acgcggtgag
    16201 cggttattat caggtcgcgc agacgctggc ggataaacgc caaaaacaac aggcgcaggc
    16261 tgccgcgcag ctggcctatt cccgtgataa caagcgtatt gatattgcgg cgaatatcgg
    16321 caccgctctg gaagcgccag gcgcgtttgc caacggcgcg gaaggcgttg ggctgtttcg
    16381 taccgaaatg ctctatatgg agcgcgacag cgcgccggac gagcaggagc aatttgaagc
    16441 ctaccagcag gtgctactgg cagcgggcga caagccgatt atcttccgca caatggacat
    16501 cggcggcgat aaaagcattc cttatctgaa tattccccag gaagagaacc cgttcctcgg
    16561 ctatcgtgcg gtacgaattt acccggaatt tgctggcctg ttccgcactc aactgcgggc
    16621 cattttgcgc gccgccagtt tcggcaacgc ccagttgatg atcccaatgg ttcacagcct
    16681 cgatcagatc ttatgggtga agggcgagat ccaaaaagcg atcgttgagc ttaagcgcga
    16741 tggcctgcgt catgcagaga cgattacgct gggtatcatg gtggaagtgc cgtcggtgtg
    16801 ctacatcatc gatcacttct gcgatgaggt agatttcttc agtatcggtt ctaacgatat
    16861 gacccagtat ctgtatgcgg tcgatcgtaa taacccgcgc gtatcgccgc tgtataaccc
    16921 gattacgcca tcgttcctgc gcatgttgca gcagattatt accactgcgc atcagcgggg
    16981 taaatgggta ggcatttgcg gtgaactggg cggtgaaagc cgttatctgc cgctactgct
    17041 tgggctgggc ctggacgagt taagtatgag tagcccgcgc attccggcgg tgaaaagcca
    17101 gcttcgtcaa ctggatagcg aggcgtgtcg ggaactggcg cgtcaggcat gtgaatgccg
    17161 cagcgcgcag gaaattgaag cgttactcac cgcctttacg ccggaagaag acgttcgccc
    17221 actgctggcg ctggagaata tctttgttga tcagtccttt agcaataaag agcaggcgat
    17281 ccagttcctg tgcggtaacc tcggcgttaa cgggcgcact gaacatccgt tcgagctgga
    17341 agaggatgtc tggcagcggg aagagattgt taccacaggc gttggttttg gcgtagcgat
    17401 cccgcacacc aaatctcagt ggatccgtca ttccagtatc agcattgccc ggctagtgaa
    17461 accggttgac tggcagtcag aaatgggcga agtcgaactg gtgatcatgc tgacactggg
    17521 cgctaacgaa gggatgaatc atgtgaaagt cttctcgcag ctggcgcgta aactggtgaa
    17581 taaaaacttc cgccagtcgc tgttcgccgc gcaagatgcg caaagcatcc tgacgctgct
    17641 ggaaacggaa ttaaccttct gaagttagcc ctgaaagcgg gcgctgtact ctcccggcgt
    17701 cagaccaaac tgacgccgga agacgcgaca aaaataatcg ctatccggaa aaccgcaacg
    17761 ctgcgccact tcatgaatcg acagatgata tttctgcaaa atcatcctcg ccttcgccat
    17821 tcgcacccag cgaacatact cgataaaacg catcgtccca tgttgggcaa acagttttga
    17881 cagatgattg ggcgtgatat taaaaaactg cgccacgctc tcgcgggtga gcggctgggc
    17941 ataattatcc tgaacccagt tacaaatgct gtgatagaga aaatcgctgc gcggctgcga
    18001 attgcccggt ggcatattca ccacgttgcg gcaaagatgc agcaaactta acaccagtgg
    18061 ctgaatgata ttctgctcca gcggtgaacg gctaagatgg gtgagggcgg taagcatcgc
    18121 ttcaccttca ccgcgttgca ggctgggaag ctggatctgg cgagcaggat gcaacaacga
    18181 cgtggtgcgg ctatcgtaaa acgataaccc aagccaggtc ggagcgaaca ccaggcttaa
    18241 cagcatcacc ggtttgttgt tgaccggtaa attagccgcg cgtgccggaa taaacagcat
    18301 ttctccttcc accatacgtt gctgctttgc ttccagcaga ttgccgtatt cgccgcgtag
    18361 cacgatatcc agacggggaa aatcaataca taaactgccc actggcaatg aggcaggctg
    18421 gctggcaaac cagacgcgcc ccagtagttg cgggttaagc accaggccgc tgaacagatc
    18481 ggcgaaaaat tgttgatcgg cgggcaagcc tggtgctttc atgaaagttc ccgattaaag
    18541 atgcattaac tggcggaatt ctttaacttt gctgcggctg accggcactt caaaatccag
    18601 atctttcagc cgcagaatat aggtgttatt aaaccacggt tcgatctcgc ggattttgtt
    18661 cagattgaca caaaatgagc gatggcagcg gaaaaaatgc gacggcggca gcttgctgca
    18721 aaactcggta atgttcatcg gcattacgta ggattcacgc cgcgtataga caaaggtcat
    18781 tttctcgtgc gcttcggcgt aatagatatc gttaattggc gtgacaatga ttcgctcatc
    18841 tttcaccaga ttaatggtgt cattctcacg cgttaccgtc gcggcaggcg tactgctggt
    18901 ctgctgttgt tgccaggctg cttccagttt ttgcagcatc ccggtaatgc gtgactcctg
    18961 atacggtttg agaatgtagt caaacgcttc cagttcaaat gcttctaccg catgttcttt
    19021 ccacgcggtg atgaacacaa taaacggttt atgggcgaac tggctgatgt tttgcgccag
    19081 caacacgcca tccagcgacg gaatattgat atccagaaaa atggcgtcga cgcggttatg
    19141 ctgcaaaaac ttcaacacgt ccagaccgtc gtcaaaggtg ccgacaatct ccatctggct
    19201 gtgctcttta attagccagc tcagttcctg ttgtgccagg aattcgtctt caacaatgat
    19261 gactttcaca atatcactcc ggctcaaagc aataacgtag cctgtgaggc gactggggtg
    19321 cgttggttag gaatgtaaaa cgcaatttcc gtccccggtt ccaggcggcg gatatgcagc
    19381 ccctcgccat acaataactt cacgcgatga tggacattca gcaggccaat tttattgccc
    19441 ggcatttcgt tcgcttcgac ccgctcaatc acctttggat cgatgccgtg cccggtatcc
    19501 cgcaccgcaa tgcgtacccg attaccgcac tctgcaacgc tgatggtgac aacgccttta
    19561 cctttgcaag gctgaatacc gtggacaatg gcgttctcca ccagcggctg gatcagcagg
    19621 ctgggaatgc agcaattcac ctcttcatca atatcataga taaccgtcag cttgtcgcca
    19681 aagcgggcct gctcaatggc aatatagtct ttaatttgat acagctcttt tttgatatcg
    19741 atttgctcat cgtcttttaa ttcaatgtta tagcgcagat aacgcgacag attaaagatc
    19801 agctggcgag cggtatccgg attcagacgg attgacgatg aaatagcgtt caaagcgtta
    19861 aacaggaaat ggggattaat tttgctttgc agggcgcgaa gctctgcctt atttgccatt
    19921 tcacgtagct gctcggcgcg tgaaacctcc agttgcgtgg agatgatttg cgacagaccg
    19981 accgccattt cctgtaatga tgacgtaatc tgatgcgcgt ggcagtagta aattttcagt
    20041 gttccggtca caacgccttt ctcccacaat gggatcacca gcatggaatg aatctctggt
    20101 gtgcggtggg cttcatcatt gtttttaatg atgatttttc cgtaattcat cgcctgtcgg
    20161 gtagtcgggc taatgaagtc atcgccattc tgatagttgt gttcacccac gccaacatag
    20221 gccagcacat gatcggtatt ggtaatcgcc accgcatcgg catgaatatc atcgcggata
    20281 atttcacaaa ctttgcgtaa tgactcgcta ttgacatggc gaaacagcgg cagcgttttg
    20341 ttggcgatat ccagcgccag cttggcctgc cgcgcggcgc tggcctcttt ttcaccctca
    20401 acactttgta ccagcaacac aataaagccg atacagacgc tgccgaggat cattggaatg
    20461 ccgattttag agacgatatc gatccccaat gcggtagttg gtgcccagac aatcaccaga
    20521 atcatggtca acgtttcaca taacatgccg ccgagaatac cgacgcgcca gcgctgtgct
    20581 ttggggattt tcaggttgat ccagcccgat atacaacccg ccagaatgct ggtgataaag
    20641 caggggatcg ccgtcacgcc gccgatatca attaaataac gatgaatacc tgcgatcacg
    20701 ccggtaataa tgcctaccca cgggccaaac agaatcccgc ctgacatcac cgcgataata
    20761 cgaacgttca ctagcgagcc ttctacggga acgccggacc aggtgctgaa cagggcaaac
    20821 agcgagaaaa tggcggtaac agcgagcaac tcttttgggg agtgcgccga cttgtgcaac
    20881 agttcgcgaa acaggcggat acggatgaga aagaacaggc agataagcat taacgctgcc
    20941 cgatcgaaga ccgccagcag catgttgaat atttcgtgca cgggtgactc tgaataagga
    21001 ttaaacctct atgataggta acctgaaggc tgatgaccag caggccgttt ttgaggaaaa
    21061 accacaatct taaaagggga ttgaaagata aatggtgtat tagca
//
LOCUS       DACBVI010000074        20839 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.074, whole genome shotgun sequence.
ACCESSION   DACBVI010000074 DACBVI010000000
VERSION     DACBVI010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.074"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            56..430
                     /locus_tag="IU325_13240"
     CDS             56..430
                     /locus_tag="IU325_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HAP5809980.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            427..621
                     /locus_tag="IU325_13245"
     CDS             427..621
                     /locus_tag="IU325_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809981.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            634..747
                     /locus_tag="IU325_13250"
     CDS             634..747
                     /locus_tag="IU325_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HAP5809982.1"
                     /translation="MQGIVPLTTETALLSCIKQAREDVLTLRHLQLLHQNR"
     gene            complement(1036..1164)
                     /locus_tag="IU325_13255"
                     /pseudo
     CDS             complement(1036..1164)
                     /locus_tag="IU325_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1236..1418
                     /locus_tag="IU325_13260"
     CDS             1236..1418
                     /locus_tag="IU325_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HAP5809983.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(1519..1848)
                     /locus_tag="IU325_13265"
     CDS             complement(1519..1848)
                     /locus_tag="IU325_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HAP5809984.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(2020..3078)
                     /locus_tag="IU325_13270"
     CDS             complement(2020..3078)
                     /locus_tag="IU325_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809985.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(3276..3749)
                     /gene="sbmC"
                     /locus_tag="IU325_13275"
     CDS             complement(3276..3749)
                     /gene="sbmC"
                     /locus_tag="IU325_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HAP5809986.1"
                     /translation="MNYEIKQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPGYFTLPENSEGVILTEITGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(3868..5034)
                     /gene="dacD"
                     /locus_tag="IU325_13280"
     CDS             complement(3868..5034)
                     /gene="dacD"
                     /locus_tag="IU325_13280"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HAP5809987.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALPDYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVANWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            5243..6670
                     /gene="sbcB"
                     /locus_tag="IU325_13285"
     CDS             5243..6670
                     /gene="sbcB"
                     /locus_tag="IU325_13285"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HAP5809988.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFNVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYIFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAIIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(6713..6940)
                     /locus_tag="IU325_13290"
     CDS             complement(6713..6940)
                     /locus_tag="IU325_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5809989.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(6954..8012)
                     /gene="yeeE"
                     /locus_tag="IU325_13295"
     CDS             complement(6954..8012)
                     /gene="yeeE"
                     /locus_tag="IU325_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HAP5809990.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHVSGLNQTLQHYSTEHNSIAETFNLSVWPLIAVL
                     LVITFWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(8191..9549)
                     /gene="plaP"
                     /locus_tag="IU325_13300"
     CDS             complement(8191..9549)
                     /gene="plaP"
                     /locus_tag="IU325_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HAP5809991.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(9539..9601)
                     /gene="yoeI"
                     /locus_tag="IU325_13305"
     CDS             complement(9539..9601)
                     /gene="yoeI"
                     /locus_tag="IU325_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HAP5809992.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(9816..10745)
                     /locus_tag="IU325_13310"
     CDS             complement(9816..10745)
                     /locus_tag="IU325_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP5809993.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(10791..11615)
                     /locus_tag="IU325_13315"
     CDS             complement(10791..11615)
                     /locus_tag="IU325_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP5809994.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGSGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGMVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(11698..11952)
                     /locus_tag="IU325_13320"
     CDS             complement(11698..11952)
                     /locus_tag="IU325_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP5809995.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(11949..12200)
                     /gene="yefM"
                     /locus_tag="IU325_13325"
     CDS             complement(11949..12200)
                     /gene="yefM"
                     /locus_tag="IU325_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HAP5809996.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            12483..12533
                     /locus_tag="IU325_13330"
     CDS             12483..12533
                     /locus_tag="IU325_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HAP5809997.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    12509..12632
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            12679..13578
                     /gene="hisG"
                     /locus_tag="IU325_13335"
     CDS             12679..13578
                     /gene="hisG"
                     /locus_tag="IU325_13335"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HAP5809998.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            13584..14888
                     /gene="hisD"
                     /locus_tag="IU325_13340"
     CDS             13584..14888
                     /gene="hisD"
                     /locus_tag="IU325_13340"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HAP5809999.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTHN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            14885..15955
                     /gene="hisC"
                     /locus_tag="IU325_13345"
     CDS             14885..15955
                     /gene="hisC"
                     /locus_tag="IU325_13345"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HAP5810000.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEISCVEQVFDSETNYILARFKASSALFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            15955..17022
                     /gene="hisB"
                     /locus_tag="IU325_13350"
     CDS             15955..17022
                     /gene="hisB"
                     /locus_tag="IU325_13350"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HAP5810001.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLNLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            17022..17612
                     /gene="hisH"
                     /locus_tag="IU325_13355"
     CDS             17022..17612
                     /gene="hisH"
                     /locus_tag="IU325_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HAP5810002.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNSWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            17612..18349
                     /gene="hisA"
                     /locus_tag="IU325_13360"
     CDS             17612..18349
                     /gene="hisA"
                     /locus_tag="IU325_13360"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HAP5810003.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYATQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            18331..19107
                     /gene="hisF"
                     /locus_tag="IU325_13365"
     CDS             18331..19107
                     /gene="hisF"
                     /locus_tag="IU325_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HAP5810004.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            19101..19712
                     /locus_tag="IU325_13370"
     CDS             19101..19712
                     /locus_tag="IU325_13370"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HAP5810005.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPQALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNITPDCDNDTLLLLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(19809..20789)
                     /gene="wzzB"
                     /locus_tag="IU325_13375"
     CDS             complement(19809..20789)
                     /gene="wzzB"
                     /locus_tag="IU325_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HAP5810006.1"
                     /translation="MRLENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVPDLQEALIGRFSSA
                     FSALAETLDNQKEPEKLTIEPSVKNQQLPLTVSYVGQTAEDAQMKLAQYIQQVDDKVN
                     QELEKDLKDNLALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTGEDITQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIH
                     AYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNSK"
BASE COUNT         5367 a         5431 c         5142 g         4899 t
ORIGIN      
        1 tttcaccacg ccacttttcc atttttcatc tctgcatatc aggaaaacct tcagtatgaa
       61 aacattaccc gatacacacg tacgggaggc atcgagctgc ccgtctcccg tcaccatctg
      121 gcagactctg ctctcccgac tactgggcca gcattacggc cttacactga atgacacacc
      181 gttcgccgat gaacgtgtga ttgagcagca tattgaggca ggcatttcac tgtgtgatgc
      241 ggtgaacttt ctcgtggaaa aatacgcgct ggtgcgtacc gaccagccgg gattcagcgc
      301 ctgtacccgc tctcagttaa taaacagtat tgatatcctc cgggcacgcc gggcaaccgg
      361 cctgatgacc cgcgacaatt acagaacggt aaataacatt accctgggta agtatccgga
      421 ggcgaaatga tgacactgga agccgacagc gttaacgtac aggcgctgga tatggggcac
      481 attgtcgttg acattgatgg tgttaatatc actgaactga ttaataaggc cgctgaaaac
      541 ggttattcac tccgcgtggt ggatgaccgt gactctaccg aaacaccggc aacttatgcc
      601 agccctcacc agttgctgta agacaatgca gtgatacagg gtatagtgcc attaaccaca
      661 gaaacagcac tgttgtcctg tattaagcag gcaagagaag atgtccttac cctgcgccat
      721 ctgcagcttc tgcaccagaa ccggtgaatc tcactcaaca ggcttcattt ctcctgacgc
      781 cagcctgaat acagctggcg ttttcattta taaacagaaa ggaaaaccga taataatgga
      841 atggacgccc cgaccatgaa gcaacggtag gatattactg ataaaccttc cagtggaggt
      901 gcgttatggc cgttttaacg attagcattg acctgacaaa aaatgaattc caaatccacg
      961 gtctgggtag gaacagaaaa atctaagctc agaaaacgga ttaaagtaag cgtcaacgga
     1021 gcaccgtatt gacgcttatt tattggtgag aactacgttc catggcagga gttcgtcaac
     1081 acggttggag ggccattccg gcagtatgct cagaatatgg cgcagatacg cttccggatc
     1141 gataccgttc agacggcagg tgcctgcttt tctggcaaca agcaggaatc tcagcgctga
     1201 agcaggaatt agaggtaaaa acgccctacc gggccatgaa tcacccggtc attggagtag
     1261 taaccaaagc agatttagcc agcatggaac aaatatcttt ggtgaaaagt tggttacggg
     1321 aagctggagc gcacaacgtg ttagtaacca gtgcagttaa taacaatgga gttacagaac
     1381 tttttgcctt gctgcataca gaagaaggct gttgttaatt tcgattggtt ctcggatgag
     1441 aatgatgacg gtgatacaca gataagaatc aatgagcaat gagggttgcc gggcaaccct
     1501 cattgaataa aacgggaatt acttcgcttc gccgtttttc atttcgccca tagctttcag
     1561 ctttttggag atatcgcggc gttctttgga gagctcggca tttttgatga tgtaatcgtc
     1621 aacgcgatct tcatagtcac ctttcatgct ggcgatgatg ccctggattg cttcaacgct
     1681 catccccggc ttgatgtaat cgctcaggtt gtccagcagc aggacgcgct tctggttgtc
     1741 acggatcttt ttctcaacgt cctgaatttc acgttgcagt ttgttcttac gacggaacag
     1801 acgaacaaat tccagtacgt cctggaatga aggcttggta gtttccattt ttatacccct
     1861 gataatgtga gagtcggatt cgtttaagca accgctattc gttagggcca acattactca
     1921 tagctgccgc agatgacaat gcttttatcc cttttcacta tcataccctt tatccttgct
     1981 gaatcgaagc agcagcaaga tgattctgaa gttcaggaat tatttgcgca aggcccggca
     2041 aatcagattc gatagcaact caagctgatg cgccgtttcc atgttcagcc acacataacc
     2101 atagattggt gtttccacaa ccttcaggtc atggtggtta ttgagcaact gacgcagctc
     2161 ttccacagca tcgttcaatt tttccgtatt ggcaaaaacc ggctgcggat taccttcgta
     2221 cagcgcatga acaaggctca gcagtatttg ctgcatcatg tgctgggtat cacgaagttt
     2281 ttgcgcgttc aataacacga aatggctggg gcgcgtggcc cagtatgcat tgatttgcaa
     2341 ctccagcata caaaccagat tgcggttaat ggtctggata ccttcatata tcgattttgg
     2401 aatacgggtt tctttgctgg cgggcgcaat cagtccacgc attttcacgg catcggtcag
     2461 gagtttttgt agatggcttt ccagacgtgg gcgttcgagt aagttcggtg agaatgcaga
     2521 ttgatagacc cgattatact cggtcagact tttcgccagt tgaatgcgcc agtggatgaa
     2581 cgcccgttgt ggccagatac cggtaaacaa cattgccagt aaagagccga ggatcacatc
     2641 gccgcttcgc cataacgccg tatcaatttc acctgtcggg gaacccacaa caattgccag
     2701 cgtcacccca atcaataaac cttgatacgg tttcttgccc agcgccagcc aaccgcaaag
     2761 gaacatggcc gccgcgcacc agactaacat cagcggtaac gagattaact ccagttgcag
     2821 agcgataaga cctaaaatcg aacccaacac cgtaccgcca atacgctcaa aggcgcgagg
     2881 gacaacgtta ccccagaacg agattggccc cataatcacc accatggtga ccagcggcca
     2941 ggtgctttcc gggatagtaa acaggcggat aatgagaaaa gtgagcagga atgccagcgc
     3001 gacccgagta ccatgcacaa tgcggtaatg gcggtatacc cggatttcaa acgggcttaa
     3061 tgacttatcg gcacgcacgc agaaactccg taatgagatt aaaagcgctc ataactcatc
     3121 tgtcggccag aaggcgcgcc tctgaattat ccgacggaca aaccgggaac agtgtaattt
     3181 cccggcagga gcgaaaaaat tgaaaggcgc atcttactct tttcgctttc aaaaaaaaga
     3241 gtggtcatcg cgttaacaca ccgccctgag atgaattagt gatgttttgg ctgcaccgca
     3301 acatacattt cgatatccca gtacccatct tccgcgccat tgttcaaata aacctcgaag
     3361 cagggctttg gtaacatttc ataagcactg tcctgcaaga ggctattaaa gaactgatac
     3421 cagggtttag caaaatcatc accgactaca cgagctaccg ccaccgcata ctgaccacct
     3481 gtaatttctg tcagaatgac gccctcactg ttttcgggaa gcgtaaagta acccggcacc
     3541 gtcacgacgg tgtcgcagcg taatttttcg gcgggtgttt catctggatt gtcgtaatag
     3601 acagcaaccc actccttcgg cacaatattt ttgctatcta cccacatcat caactgctca
     3661 aagcctttct ttaccgtctg ttcccacggg ccaacgagat ggaaacctgc aacggtacgt
     3721 ttctcttcct gcttaatctc gtagttcatg acgcctccat tgatactgtt tttatacaca
     3781 gtatagttgc aaaattaaaa ccacaaggaa tgagtgttga ttatgcgagc agactcgcac
     3841 tcctgccagt ctgctgcaaa agaaaggtca ggccttatgg tggaaataat cactcaggcg
     3901 agaaaacatg ctgccttccc cgacagattc cagggtaacc agcggccagt tcgccacctg
     3961 tttatcacgg tcgtaaagtt caatttcccc tacccgctga tgggcgctaa ttggcgcggt
     4021 gagttcttta ccatcaaggg tatatttggc tttgatatgt ggaatttcgg ctttcggtag
     4081 taccatccag aactcttgtt ctgttcccag ggcgatattt tctttatcgc cataccatat
     4141 gcgttccgta ccgacctttt tcccacggtg caaaatttgc accgtagtaa agttttgttg
     4201 cccccaacgc agtaattttc ttgcctcttc ctcacgacct tttgcgctgt cagcacccat
     4261 taccactgca atgagacgac gctgcccatc tacagccgaa gcaatgagat taaacccagc
     4321 accagaagta tgacccgttt tcaggccgtc aacattcatg gttttatccc acaataaccc
     4381 gttacggttt tgctgggtga taccgttcca ggtgaggctt ttctcactgt acatatgata
     4441 aaactcgggc tcgccgtgga tgatagcacg agaaagcaca gctaaatcat aagccgagct
     4501 atgctgacct ggcgcatcca ggccatgcac tgtttcaaaa tgcgtatcct tgagatgcag
     4561 cttctcggca tagttgttca tcatttcaac aaactgccgt tgcccaccgg caatatagtc
     4621 aggcagtgca acacaagcgt catttccgga atccacaatt aaaccacggc ttaaatcacg
     4681 taccgatacg cgatcgccct ctttcaaaaa catcagtgaa gaaccgacaa acaccggatt
     4741 atctttcgcc cacgcatcgc gccccacggt gacaatatcg tctggcgtaa tgcgatgact
     4801 atcgatagcg cgatccacga cataacccgt catcagcttt gtcaggctgg cgggattgcg
     4861 ctgttgatgc tcattacccg cggtgaggat ctgaccggtg gtgtaatcca tcagtaccca
     4921 ggacccggca tgaatctctg gaggctgagg tgaaaaagga atgttttccg ccgcaaaacc
     4981 agacgataag ttaaaaacga acaaagaagc agcaataata agacggcgtt tcaacagcaa
     5041 accctcagga gtttcaaata gctgttcttt ttacggaaat acttatgaac tggctggaat
     5101 aaagtgcaag aaaatgtgac taccctctca tttttatctg acatgatctg ttgccactcg
     5161 ctgccaaatt gtggcgctaa agctgattag cacggtgata tttgatactc tggcagacag
     5221 cagaaataac ggatttaacc taatgatgaa tgacggtaag caacaatcta cctttttgtt
     5281 tcacgattac gaaacctttg gcacgcaccc cgcgttagat cgccctgcac agttcgcagc
     5341 cattcgcacc gatagcgaat tcaatgtcat cggcgaaccc gaagtctttt actgcaagcc
     5401 cgcggatgac tatttgcccc agcctggagc agtattaatt accggtatta ccccgcagga
     5461 agcacgggcg aaaggagaaa acgaagccgc gtttgccgcc cgtattcact cgctttttaa
     5521 cgtaccgaag acctgtattc tgggctacaa caatgtgcgt ttcgacgacg aagtcacacg
     5581 caacgttttt tatcgtaatt tctacgatcc ttacgcctgg agctggcagc atgataactc
     5641 gcgctgggat ttgctggatg ttatgcgtgc ctgttatgcc ctgcgcccgg aaggaataaa
     5701 ctggcctgaa aatgatgacg gtctaccgag ctttcgcctt gagcatttaa ccaaagcgaa
     5761 tggtattgaa catagcaacg cccacgatgc tatggctgat gtgtacgcca ctattgcgat
     5821 ggcaaagctg gtaaaaacgc gtcagccacg cctgtttgat tatatcttta cccatcgcaa
     5881 taaacacaaa ctgatggcgt tgattgatgt tccgcagatg aaacccctgg tgcacgtttc
     5941 cggaatgttt ggggcatggc gcggcaacac cagctgggtg gcaccgctgg cgtggcatcc
     6001 tgaaaatcgc aatgccataa ttatggtgga tttggcagga gacatttcgc cattactgga
     6061 actggatagc gacacattgc gcgagcgttt atataccgca aaaaccgatc ttggcgataa
     6121 cgccgccgtt ccggttaagc tggtgcacat caataaatgt ccggtgctgg cccaggcgaa
     6181 tacgctacgc ccggaagatg ccgaccgact gggaattaat cgtcagcatt gcctcgataa
     6241 cctgaaaatt ctgcgtgaaa atccgcaagt gcgtgaaaaa gtggtggcga tattcgcgga
     6301 agccgaaccg tttacgcctt cagataacgt ggatgcacag ctttataacg gctttttcag
     6361 tgacgccgat cgcgcagcaa tgaaaattgt gctggaaacc gagccgcgta atttaccggc
     6421 gctggatatc acttttgttg ataaacgaat tgaaaaactg ttgttcaatt atcgggcacg
     6481 caacttcccg gggacgctgg attatgccga gcagcaacgc tggctggagc accgccgcca
     6541 ggtcttcacg cccgagtttt tacaaggtta tgcagatgaa ttgcagatgc tggcacaaca
     6601 atatgccgat aacaaagaga aagtggcgct gttaaaagcg ctttggcagt acgcggaaga
     6661 gatcgtctaa tttgaagcct ccgccgctgg tacggcggag gtcaatgttg agtcaggctt
     6721 tttgaacggt gatactccac gctgcatcgc caatctgctg ataatcggtg atggcatgcc
     6781 cttcttctgc cgcccactgt gggattgctt cagttgcctg ggtgcaatca aactcaatta
     6841 ccaactcatc gccgctggcc atttctgcca gtgctgcttt tgcttctatg agtggaaacg
     6901 gacagacctg cgtcaccaca tccagctttt taatcgccat ctttacacct tacttaattt
     6961 gccgcagcag ttgccagtcg cgccttacgc tgcggtcgaa catacacaag ccaggacgca
     7021 gtccagactc cgagaatcat aaatacaagg ccaatccagc cctgccaggt catcattgca
     7081 gtcataacca gtccattacc gatagagcaa ccacctgcaa tactggcacc gaagcccatc
     7141 agtacaccac ctaacccgct acgtaatgtt gtttgtgcat cagctgcgcg aacgcggaac
     7201 tcacggctcg ctttggctgc aataaatgac cccacgaaga tccctaacac caggaaaacg
     7261 ccccagttaa tgtatttcac gtcacccgcg accagaaatt gcagaatatt ggccgttggg
     7321 gaagtgattc ctaacccaaa catgcgtccg gttgcttcac tcaggggcca ggctaaaagc
     7381 gcaatcaaac cgatgagtac agcagtgaca aagggatgcc agcgtttttc aaacagaata
     7441 tgagcgatcc cggttcggcg cggcggtaag gtcgcgactt tgagttttgg cttcttcaac
     7501 tctttcatca ccacccagaa agttatcacc agcaaaacgg caatcaacgg ccacacagac
     7561 agattaaacg tctcggcaat ggagttatgt tcagtactgt agtgctgcaa ggtttgattt
     7621 aaaccactga catgtggaga acgcatcacc gcactcatca ccatataagt gaaaagcgcg
     7681 atccaactgc cgatcaatcc ctcacccgcg cgataccagg tcccggtggc acatccaccc
     7741 gccagaacaa ttcccagccc gaagatatac ccacctataa cagtaccaag ccacgggaac
     7801 gccccggctt cgtaagtcag taggcccgcc tgaattaacg caaaaacccc cacgctttgt
     7861 accgaaattg caatcagcaa ggcgtaaaac atgcgattat ttttcgcgat atacatatcg
     7921 cgaaaaccac ctgtcaggca aaaacgccca cgctgcatga caaatcccag cagagcacca
     7981 caaattagcc cgcttaatat cattgaaaac ataaattaat taaccagatg aatgttaatg
     8041 gggaaattat tcatgactgg tggaatgcag accaataacc aaattgtcta aattagaaca
     8101 aatggttatt aatgaggatg tcgataaaaa agaataaaaa aaccggagcc aaatattcat
     8161 tcgactccgg tttattatta gaaaagatga ttacgctacg tcttcgtact gcggaaccgg
     8221 gttacggaaa ctcttggtga cgcaagccag gtaaatcaga ccgatagcag cccagatcag
     8281 acccagaacc attgagcttt cttccaggtt aacccacagc gcaccaacgg tcagtgcacc
     8341 acacatcggc aggaacagat actggaagtg atctttcagc gtcttattac gcttctcacg
     8401 gatccagaac tgcgagatga ccgacaggtt aacgaaggtg aacgccacca acgcaccaaa
     8461 gttaatcagc gctgtagcca ttaccaggtc gaagttgatt gccagcaggg caatcgcgcc
     8521 aaccaggatg atgttcatcg ccggagtacg ccatttcggg tgtacataac cgaagaagct
     8581 tttcgggaac acgccgtcac gacccattac gtacatcaga cgcgcaacac ctgcgtgcgc
     8641 cgccataccg gacgccagta cggtaatggt ggagaagatc agcgcaccaa cctggaatgc
     8701 tttacctgcc acgtacagca tgatttcagg ctgtgacgca tccggatctt taaagcgaga
     8761 gatatccggg aagtacagct gcaggaagta agttgcaaag atgaagatca tgccgccaat
     8821 cagcgcggtc aggaaaatcg cacgcgggat cacgcgctct gcatctttgg tttcttccga
     8881 caggttgctg atgccgtcaa agccggtaaa ggagaagcac aggattgtcg ccccggtaat
     8941 catcgggata acatgcgcat cgccagacca gaacggacga gtgctcgcca gcgtaccagc
     9001 gccttcacct tcaaatacgc cataaacaac catgcccaga atcaccgcga tcagcactac
     9061 ctgcaacacg acgattacag tgttgaagtt cgctacggat ttcagactac gcaggttaaa
     9121 ggcggtcata aaggccacca gcgccaccac aaacatccac gatgggatgg aaggcaccag
     9181 agcttcaaaa tagattttcg ccagcagaat gttgatcatc ggcgcgaaca gatagtcgag
     9241 cagagaagac caacccacca taaagccgac agtcgggcta atggatttct gggcgtaagt
     9301 atatgcagag ccagcagaag gatagcggcg aaccagcttc ccgtagctca gagccgtaaa
     9361 caggatcgca atcaatgcga acgcataggc tgtcggcaca tgaccatccg tgaggcctga
     9421 aacgatacca aaagtatcaa acagcgtcat cggctgcata taggcaagac ccatcattac
     9481 aaccggaact aacgtaagcg ttttacgcaa ttccacgcga gaggtgtttg gagtaacgtt
     9541 atgcgacatg gtcattctcc tttacggtga taaccgtcgc gtaagcaaaa aattgcccca
     9601 tttttttgga ttcctcagcg acaacaactg tcgattttta gtaaatatct atccggtacg
     9661 aagcccggcc tcttggtatg aatgattggt ttgaagcaaa aaataaccga cgctgatgaa
     9721 acgtcggttt ttagtcattt tttgacagcg gcgcattgtg cctaaatggg ggggaaatga
     9781 caagagaatg agaggcttgt cataataatt tttctttaaa tggctgattt tccgtcatca
     9841 gattttgcat aaacaccggc gatcgcacta tttgctaaaa tttcatctcg ccaccagccg
     9901 ctggcaagcc ctgcggtctg ttcgttccag ccaatccaga cgggttcaaa gagagtttgg
     9961 gcgaccactt tcttctcgat taacgcgcca ctatcgagaa aacgttgcgc cagataacgg
    10021 ggtaaatagc cgcagccgag gccgctaatt tgcagctcca gcttggtttt aaaatcgaag
    10081 acggtaatgg cttcttgttc gtcgagaagt tgtgaggccg tagaagcgcc ggcctgcgca
    10141 gtgtccccca ccacaatcgc ccgatatcgc ttaatgatac gacggtttaa cggttcttct
    10201 tccagggcta acggatgatg aggcgctacc gcaaagactt gttccagatc gcccaaccgc
    10261 gagaaaccaa actcactgga agatggtggc tcatgcatcg cgccaacaat aatatccgct
    10321 cgcccctgag tcagggcatc ccatgaacca ccgagtacgc cattgataaa cttcaggcgc
    10381 gtcacgctat gatgttgata aaaagcttca atgagtggcg caagcagaga aaaaggaaac
    10441 gtgtcgtcca cgccaatcac cagttcgttt tcccagcctt catgcagttt gatcgcctgc
    10501 ttttccagtt ctcgcacggt atgcagaact tcccgccctt tctctaataa catttttccg
    10561 gtgcgggtga atttagcgcg gtgtccgcta cgatcaagca attgaatatt aaggtcgctt
    10621 tccagcttgt gaacggtgta actcagagcg gacggcgtct tatagagttt ggccgacgcc
    10681 gccgcaaaac tgccttcttt ttctaaggca tcgaggatca tcaacacatc cagcagtggt
    10741 ttcatacacg cccccttgca gtgcatgcgg cgtcctgctg gcgtggtcgt ttactccagc
    10801 ggcattacca gcggatcggg atactggtat tcaaatccca gttcattaca aatccgactg
    10861 ccatcaataa tcttaccttt gccgctgtcc agactatttc tgaactgcgg aggctccagc
    10921 cccagtaaac gggccatctg cggatagaaa acattacgcg cagggtgagc tggcgcacat
    10981 atattataga tgtgtccgcc tttaggtgcc tgtaacaaca gagtgatagc gccaataaca
    11041 tcttctaaat ggactaaatt aacaccatgt tcaccatcag gcgcggtttt tccggcaaag
    11101 aagcgtccgg gatgacgccc cggtccgacc aggcccgcaa gacgcagaat atcgaccgaa
    11161 gtaccgggta aattgtgcag ccagtcttcg agttcttcta atactcgtcc actgttggtt
    11221 accggattac gcggggtagt ttctttcacc atgccttgcg catcgccata gacagatgtt
    11281 gagctggtaa aaataattcg gggaatacga tgggccagcg cgctatccac taactcttgt
    11341 accgcttgta aatagaactc atcgccggag ccgctacgac gtgccggaag cgtaatgacc
    11401 agtgcatcgg catccatcag ggcatccaga tcgtcagaat cgcaaactaa ctcaggctcc
    11461 atgcgaagca gatagctatc aatgccactc attcgggccg cttcgacacc atcttgtgtg
    11521 gttttactcc cggtgacttg ccagcctcgc gctgaaagtg acatcgccag cggcatgcct
    11581 aaccacccta acccgacaat tgcgaccttt ttcatccgtt atctcctgat acaccttaga
    11641 tctataaggc tacgctagcg tatcaaaact gacaattcat tctatgaatg aatctgttca
    11701 ataatgataa cgacatgctg caatgagcag tgaatcgtcg gtaaccgcgt ataccagacg
    11761 gtgctcctct gtaatgcgtc gggaccagaa acctgacaaa ttatgtttca ggggttctgg
    11821 cttcccctta ccttcaaatg gcgttctgcg ggtatctttg ataagttcat tgatcttttt
    11881 aacaattcgc ttatctgttt cctgccagta cagataatca tcccatgatt cctcagacca
    11941 gattagtttc actcaataat gtccttttct gttcctttgc ctgatttcag gctatcgatt
    12001 gagtccatca atctccgggc gttagcgggg gaacgcagta gataagccgt ctcttccagc
    12061 gagttgtatt cttcgagtga catcagaaca caagcctctc cattctgacg agtaataagg
    12121 atcggggcat gatcttcaac ggctttcatc attgttgccg acaaattctg acgcgcttcg
    12181 ctgtagctaa ttgtacgcat gtcaatctcc tcttttgtac agttcattgt acaatgatgg
    12241 gcgttaatta actatttatt aattagtttg tagatcaagg tattgtcagt gagacgaaaa
    12301 tccaggcttc gctatttttg gtgccgtcag cttagaggac agtcctctta gcgccctcct
    12361 ttccccgctc attcattaaa caaatccatt gccataaaat atataaaaaa gcccttgctt
    12421 tctaacgtga aagtggttta ggttaaaaga catcagttga ataaacattc aaagagactt
    12481 ttatgacacg cgttcaattt aaacaccacc atcatcacca tcatcctgac tagtctttca
    12541 ggcgatgtgt gctggaagac attcagatct tccagtggtg catgaacgca tgagaaagcc
    12601 cccggaagat caccttccgg gggctttttt attgcgcgat tgataacggt tcagacaggt
    12661 ttaaagagga ataagaaaat gacagacaac actcgtttac gcatagctat gcagaaatcc
    12721 ggccgtttaa gtgatgactc acgtgaattg ctggcgcgct gtggcattaa aatcaatctt
    12781 cacacccagc gcctgatcgc gatggcagaa aacatgccga ttgatattct gcgcgtgcgt
    12841 gacgacgaca ttcccggtct ggtaatggac ggcgtggttg acctcgggat tatcggcgaa
    12901 aacgtgctgg aagaagagct gcttaaccgc cgcgcccagg gtgaagatcc acgctacttt
    12961 accctgcgtc gtctggattt cggcggctgc cgtctttcgc tggcaacgcc ggttgatgaa
    13021 gcctgggatg gcccgctctc cttaaacggt aaacgtatcg ccacctctta tcctcacctg
    13081 ctcaagcgtt atctcgacca gaaaggcatc tcttttaaat cctgcttact gaacggttct
    13141 gttgaagtcg ccccgcgtgc cggactggcg gatgcgattt gcgatctggt ttccaccggt
    13201 gccacgctgg aagcaaacgg cctgcgcgaa gtcgaagtta tctaccgctc gaaagcctgc
    13261 ctgatccagc gcgatggcga aatggaagaa tccaaacagc aactgatcga caaactgctg
    13321 acccgtattc agggtgtgat ccaggcgcgc gaatcaaaat acatcatgat gcacgcaccg
    13381 accgaacgtc tggatgaagt catcgccctg ctgccaggtg ccgaacgccc aaccattctg
    13441 ccgctggcgg gtgaccaaca acgcgtagcg atgcacatgg tcagtagcga aaccctgttc
    13501 tgggaaacga tggaaaaact gaaagcgctg ggtgccagtt caatcctggt cctgccgatt
    13561 gagaagatga tggagtgatc gccatgagct ttaacacaat cattgactgg aatagctgta
    13621 ctgcggagca acaacgccag ctgttaatgc gcccggcgat ctccgcttct gaaagcatta
    13681 cccgcactgt taacgatatt ctcgataacg tgaaagcacg cggcgatgag gccctgcggg
    13741 aatacagcgc gaagtttgat aaaaccacgg ttaccgcact gaaggtgtct gctgaggaaa
    13801 ttgccgccgc tagcgaacgc ctgagcgacg agctaaaaca ggcgatggcg gtggcagtaa
    13861 agaatattga aaccttccac actgcgcaaa aactgccgcc ggtagatgta gaaacgcagc
    13921 caggcgtgcg ttgccagcag gtcacgcgcc cggtcgcttc agttgggttg tatattcctg
    13981 gcggctccgc cccgctcttc tcaacggtat taatgctggc gactccggcg cgtattgcgg
    14041 gctgtaaaaa agtggtgctg tgctcaccgc cgccgattgc cgatgagatc ctttatgcgg
    14101 cgcagctgtg cggtgtgcag gacgtgttta acgtcggcgg cgcacaggcc attgccgcac
    14161 tggcgtttgg tacggaatct gtgccgaaag tggacaaaat cttcgggccg ggcaacgcct
    14221 ttgtcaccga agcgaaacgt caggtgagcc agcgtctgga cggtgcggcg atcgatatgc
    14281 ccgcaggccc gtcggaagtg ttggtcattg ccgacagtgg cgcaacgccg gatttcgtgg
    14341 cttctgattt gctttctcag gctgaacacg gcccggactc acaggtgatt ttactgacgc
    14401 ccgctgctga tatggcgcgt cgcgttgccg aggctgtcga acgccagctg gcagaactgc
    14461 cgcgtgctga aaccgcccgt caggcactga acgccagccg cctgatcgtg actaaagatt
    14521 tagcgcagtg cgtagagatc tccaaccagt acggcccgga gcacctgatc atccagaccc
    14581 acaacgcccg cgatctggtc gatggcatca ccagcgccgg ttcggtattt cttggtgact
    14641 ggtcaccgga atcggcaggc gactatgcct ccggcactaa ccatgttcta ccgacttacg
    14701 gttacaccgc cacctgttcc agcctcgggc tggcagattt ccagaagcgc atgaccgtgc
    14761 aggaactctc gaaagaaggc ttttccgcgc tggcttcgac cattgaaaca ctggccgccg
    14821 ccgagcgcct gaccgcccat aaaaatgccg ttactttgcg tgttaacgcc cttaaggagc
    14881 aagcatgagc accgtgacta ttaccgattt agcgcgtgaa aacgtccgca acctgacgcc
    14941 gtatcagtcg gcgcgtcgtc tgggcggtaa cggcgatgtc tggctgaacg ccaacgaata
    15001 tcccacagcc gtggagtttc agctcactca acaaacgctc aaccgctatc cggaatgtca
    15061 gccgaaagcg gtgattgaaa attacgcgca atatgcaggc gtaaaaccgg aacaggtgct
    15121 ggtcagccgt ggcgcggacg aaggtattga actgctgatc cgcgcttttt gcgaaccagg
    15181 taaagatgcc atcctctact gcccgccaac gtacggcatg tacagcgtca gcgccgaaac
    15241 cattggcgtc gagtgccgca cagtgccgac gctggaaaac tggcaactgg acttacaggg
    15301 catttccgac aagctggacg gcgtaaaagt ggtctatgtt tgcagcccca acaacccaac
    15361 cgggcaactg atcaatccgc aggattttcg caccctgctg gagttaacgc gcggtaaggc
    15421 gattgtggtt gccgatgaag cctatataga gttttgtcca caggcatcgc tggctggctg
    15481 gctggcggaa tatccgcacc tggctatttt gcgcacactg tcgaaagctt ttgctctggc
    15541 ggggcttcgt tgcggattta cgctggcaaa cgaagaagtc atcaacctgc tgatgaaagt
    15601 gatcgccccc tacccgctct cgacgccggt tgccgacatt gcggcccagg cgttaagccc
    15661 gcaggggatc gtcgccatgc gcgaacgagt ggcgcaaatt attgcagaac gtgaatatct
    15721 gattgccgca ttgaaagaaa tctcctgcgt ggagcaggtt tttgactctg aaaccaacta
    15781 cattctggcg cgctttaaag cctccagtgc attgtttaaa tctttgtggg atcagggcat
    15841 tatcttacgt gatcagaata aacaaccctc tttaagcggc tgcctgcgaa ttaccgtcgg
    15901 aacccgtgaa gaaagccagc gcgtcattga cgccttacgt gcggagcaag tttgatgagt
    15961 cagaagtatc tttttatcga tcgcgatgga accctgatta gcgaaccgcc gagtgatttt
    16021 caggtggacc gttttgacaa actcgccttt gaaccgggcg tgatcccgga gctgctaaag
    16081 ttgcaaaaag cgggctacaa actggtgatg atcactaatc aggatggtct gggaacacaa
    16141 agtttcccgc aggcggattt cgatggcccg cacaacctga tgatgcagat cttcacctcg
    16201 caaggcgtgc agtttgatga agtgctgatt tgccctcacc tgcccgccga tgagtgcgac
    16261 tgccgtaagc cgaaagtaaa actggtggag cgttatctgg ctgagcaagc gatggaccgt
    16321 gccaacagtt atgtgattgg cgatcgcgcg accgacattc aactggcgga aaacatgggc
    16381 attaatggtt tacgctacga ccgcgaaatc ctgaactggc cgatgattgg cgagcaactc
    16441 actaaacgag accgttacgc ccatgtagtg cgcaacacca aagagacgca aattgacgtc
    16501 caggtgtggc tggatcgcga aggtggcagc aagattaaca ccggtgttgg cttctttgat
    16561 cacatgctgg atcagatcgc cacccacggc ggtttccgca tggaaatcaa cgtcaaaggc
    16621 gacctctata tcgacgatca ccacaccgtc gaagataccg gcctggcgct gggcgaagcg
    16681 ttaaaaattg ccctcggtga caaacgcggt atttgccgct ttggttttgt gctgccgatg
    16741 gacgaatgcc ttgcccgttg cgcgctggat atctctggtc gcccgcacct ggaatataaa
    16801 gccgagttta cctaccagcg cgtgggcgat ctcagcaccg aaatgatcga gcacttcttc
    16861 cgttctctct cttacaccat gggcgtgacg ctaaacctga aaaccaaagg taaaaacgat
    16921 catcaccgtg tagagagcct gttcaaagcc tttggtcgga ccctgcgcca ggccatccgc
    16981 gtggaaggcg acaccctgcc ctcgtcgaaa ggagtgctgt aatgaacgtg gtgatccttg
    17041 ataccggctg cgccaatttg aactcagtga agtctgccat tgcgcgtcac ggttatgaac
    17101 ccaaagtcag ccgtgacccg gacgtcgtgt tgctggccga taaactattt ttacccggcg
    17161 ttggcactgc acaagcagcg atggatcagg tgcgtgagcg cgagctgttt gatctcatca
    17221 aagcctgtac ccaaccggtg ctgggcatct gcttagggat gcaacttctg gggcggcgca
    17281 gcgaagagag caacggcgtc gatttgctgg gcatcatcga cgaagacgtg ccgaaaatga
    17341 ccgactttgg tctgcccctg ccgcatatgg gctggaaccg cgtttaccca caggcaggca
    17401 accgcctgtt tcaggggatt gaagacggcg cgtactttta ctttgttcac agctacgcaa
    17461 tgccagtcaa ttcgtggacc atcgcccagt gtaattacgg cgaaccgttt accgcggcgg
    17521 tacaaaaaga taacttctac ggcgtgcagt tccacccgga gcgttctggt gccgctggtg
    17581 ctaagttgct gaaaaacttc ctggagatgt gatgattatt ccggcattag atttaatcga
    17641 cggcaccgtg gtgcgtctcc atcagggcga ttatggcaaa caacgcgatt acggtaacga
    17701 cccgctgcca cgcttacagg attacgccac acaaggtgct gaagtgctgc accttgtgga
    17761 tctgaccggg gcaaaagatc cggctaaacg tcaaatcccg ctgattaaaa ccctggtcgc
    17821 gggcgttaac gttccggtgc aggttggcgg cggcgtgcgt agcgaagaag acgtggcggc
    17881 gttactggaa gcgggcgttg cgcgcgtggt ggttggctcc accgcggtga aatcaccaga
    17941 aatggtgaaa ggctggtttg aacgtttcgg tgccgatgcc ttagtgctgg cgctggatgt
    18001 ccgtattgac gagcaaggca acaagcaggt ggcggtcagc ggctggcaag agaactcggg
    18061 cgtgtcgctg gaacaactgg tggaaaccta tctgcccgtc ggcctgaaac atgtgctgtg
    18121 taccgatatc tcacgcgacg gtacgctggc aggctctaac gtctctttat atgaagaagt
    18181 gtgcgccaga tatccgcaag tagcatttca gtcctccggc ggcattggcg acattaacga
    18241 tgttgctgcc ctgcgtggaa ctggcgtgcg cggcgtaata gttggtcggg cattactgga
    18301 aggtaaattc accgtgaagg aggccatcgc atgctggcaa aacgcataat tccatgtctc
    18361 gacgttcgtg atggtcaggt ggtgaaaggc gtacagtttc gcaaccatga aatcattggc
    18421 gatatcgtac cgctggcaaa acgctacgcc gaagaaggtg cagacgaact ggtgttctac
    18481 gatatcaccg cttccagcga tggccgtgtg gtagataaaa gctgggtatc gcgcgtggcg
    18541 gaagtgatcg acattccgtt ctgcgtggcg ggcgggatta agtctctgga agatgccgcg
    18601 aaaattcttt cctttggcgc ggataaaatc tccattaact cccctgcgct ggcggacccg
    18661 acattaatca ctcgcctggc ggatcgcttt ggcgtgcagt gtattgtggt cggtattgat
    18721 acctggtacg acgctgaaac cggtaaatat catgtgaatc aatataccgg cgatgaaagc
    18781 cgcacccgcg tcactcagtg ggaaacgctc gactgggtac aggaagtgca aaaacgcggt
    18841 gccggagaaa tcgtcctcaa tatgatgaat caggacggcg tgcgtaacgg ttacgatctc
    18901 gaacaactga aaaaagtgcg tgaagtttgc cacgtcccgc tgattgcctc cggtggcgcg
    18961 ggcaccatgg aacacttcct cgaagccttc cgtgatgccg acgttgacgg cgcgctggca
    19021 gcttccgtat tccacaaaca aataatcaat attggtgaat taaaagcgta cctggcaaca
    19081 cagggcgtgg agatcaggat atgttaacag aacaacaacg tcgcgaactg gactgggaaa
    19141 aaaccgacgg acttatgccg gtgattgtgc aacacgcggt atccggtgaa gtgttaatgc
    19201 tgggctatat gaacccgcaa gccctggaca aaaccatcga aagcggcaaa gtcaccttct
    19261 tctcgcgcac taaacagcga ctgtggacca aaggcgaaac gtcgggcaat ttcctcaacg
    19321 tagtaaatat tacgccggac tgcgacaacg acacgttact gttgctggcg aatcccatcg
    19381 gcccgacctg ccacaaaggc accagcagct gcttcggcga caccgctcac cagtggctgt
    19441 tcctgtatca actggaacaa ctgctcgccg aacgtaaatc tgccgacccg gaaacctcct
    19501 ataccgccaa actgtatgcc agcggcacca agcgcattgc gcagaaagtg ggcgaagaag
    19561 gcgtggaaac cgcactggca gcgacagtac atgaccgctt tgagctgacc aacgaggcat
    19621 ctgatttgat gtatcacctg ctggtgctgt tgcaggatca ggatctggac ttaacgacgg
    19681 tgattgagaa cctgcgtaaa cggcatcagt gagttgcggg gtaagcggat gcgatattgt
    19741 cgtcgcatcc ggcaaaaaaa acgggcaagg tgtcaccacc ctgcccattt tctttaaaac
    19801 cgaaaagatt acttcgagtt gtaattacgc agagcattac gccccaacac aatccccgcg
    19861 ccaaccatgc cgcccagcag caccgccaga atcaaggtaa ttgccttttt cgggctatcg
    19921 cgacgaatag gtaacgtcgg tttcatcaca taccggtaag catgaatatc aagatcgtca
    19981 acctttaagt tttcgatatc cagcaggttt tgacgagtct gatagtagtt tggcgaaaac
    20041 accaacggac gagtcgcctc atgcttaatc atcgactcca gcgcttcgct ccccaaaagg
    20101 aacaacgtat cttgtgtgat atcttcgcca gtctgttgaa tctgcggttt tgtcacctgc
    20161 gcctgattcg catactgcaa cgcttcctga atctgacgga tacgcagatc tttctgctcc
    20221 tgcgcaacca cttcctgagt tcttaaagag tcctgcaagt ttttccgtcc caaagcaagg
    20281 ttgtccttga gatccttttc tagctcttga tttactttat catcaacttg ctgaatgtat
    20341 tgggccaatt tcatttgtgc gtcctctgca gtttgcccaa cataagaaac agtcaatggt
    20401 aattgctggt tctttacaga aggttcgatg gtaagttttt ctggttcttt ctgattatcc
    20461 agcgtttctg ctaatgcaga gaaggcagaa ctgaagcgac caattaacgc ctcctgtaaa
    20521 tccggcactt taggtgcagc ctgaccatag ataacattca tggcattgtt atagccagca
    20581 atttgcccca cgtcaggctg agtgataatt gctgttgacg tccatttctc cttcgctacc
    20641 gccaaatacc caatagccag ggcaatagca acaatgacag agatgataat tgtcatcttg
    20701 ccacgccaca actgcaccag taaatcaatc aaatcaatct gttccgggtc atggttttgc
    20761 ccagaaacat tattattttc taatctcatc attaccctaa cggacgaaat aattttaagt
    20821 cgccatagtt tagcgataa
//
LOCUS       DACBVI010000075        20823 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.075, whole genome shotgun sequence.
ACCESSION   DACBVI010000075 DACBVI010000000
VERSION     DACBVI010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.075"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..1137
                     /gene="proV"
                     /locus_tag="IU325_13380"
     CDS             <1..1137
                     /gene="proV"
                     /locus_tag="IU325_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HAP5810007.1"
                     /translation="KYIEQGLSKEQILEKTGLSLGVKDASLAIEEGEIFVIMGLSGSG
                     KSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKIAMVFQSFALMPHMTVL
                     DNTAFGMELAGINAEERREKALDALRQVGLENYAHSYPDELSGGMRQRVGLARALAIN
                     PDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMRIGDRIAIMQN
                     GEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRTPNGLIRKTPGFGPRS
                     ALKLLQDEDREYGYVIERGNKFVGAVSIDSLKAALTQQQGLDAALIDAPLAVDAQTPL
                     SELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG"
     gene            1130..2194
                     /gene="proW"
                     /locus_tag="IU325_13385"
     CDS             1130..2194
                     /gene="proW"
                     /locus_tag="IU325_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HAP5810008.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2252..3244
                     /gene="proX"
                     /locus_tag="IU325_13390"
     CDS             2252..3244
                     /gene="proX"
                     /locus_tag="IU325_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HAP5810009.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYGLTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            3697..4881
                     /locus_tag="IU325_13395"
     CDS             3697..4881
                     /locus_tag="IU325_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5810010.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            5005..5742
                     /locus_tag="IU325_13400"
     CDS             5005..5742
                     /locus_tag="IU325_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP5810011.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            5732..6067
                     /gene="ygaH"
                     /locus_tag="IU325_13405"
     CDS             5732..6067
                     /gene="ygaH"
                     /locus_tag="IU325_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HAP5810012.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAIGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(6086..6187)
                     /locus_tag="IU325_13410"
     CDS             complement(6086..6187)
                     /locus_tag="IU325_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810013.1"
                     /translation="MFNGRKRTIHLGMTSLISADIVTTVTNKVNVLF"
     gene            6158..6688
                     /gene="emrR"
                     /locus_tag="IU325_13415"
     CDS             6158..6688
                     /gene="emrR"
                     /locus_tag="IU325_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HAP5810014.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            6815..7987
                     /gene="emrA"
                     /locus_tag="IU325_13420"
     CDS             6815..7987
                     /gene="emrA"
                     /locus_tag="IU325_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HAP5810015.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            8004..9542
                     /gene="emrB"
                     /locus_tag="IU325_13425"
     CDS             8004..9542
                     /gene="emrB"
                     /locus_tag="IU325_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HAP5810016.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(9606..10121)
                     /gene="luxS"
                     /locus_tag="IU325_13430"
     CDS             complement(9606..10121)
                     /gene="luxS"
                     /locus_tag="IU325_13430"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP5810017.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(10271..11827)
                     /gene="gshA"
                     /locus_tag="IU325_13435"
     CDS             complement(10271..11827)
                     /gene="gshA"
                     /locus_tag="IU325_13435"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAP5810018.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDIAGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSKELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(11900..12328)
                     /locus_tag="IU325_13440"
     CDS             complement(11900..12328)
                     /locus_tag="IU325_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAP5810019.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12325..12891)
                     /gene="yqaB"
                     /locus_tag="IU325_13445"
     CDS             complement(12325..12891)
                     /gene="yqaB"
                     /locus_tag="IU325_13445"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HAP5810020.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(13039..13115)
                     /locus_tag="IU325_13450"
     tRNA            complement(13039..13115)
                     /locus_tag="IU325_13450"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13079..13081),aa:Arg,seq:acg)
     gene            complement(13218..13294)
                     /locus_tag="IU325_13455"
     tRNA            complement(13218..13294)
                     /locus_tag="IU325_13455"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13258..13260),aa:Arg,seq:acg)
     gene            complement(13357..13433)
                     /locus_tag="IU325_13460"
     tRNA            complement(13357..13433)
                     /locus_tag="IU325_13460"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13397..13399),aa:Arg,seq:acg)
     gene            complement(13632..13708)
                     /locus_tag="IU325_13465"
     tRNA            complement(13632..13708)
                     /locus_tag="IU325_13465"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13672..13674),aa:Arg,seq:acg)
     gene            complement(13712..13804)
                     /locus_tag="IU325_13470"
     tRNA            complement(13712..13804)
                     /locus_tag="IU325_13470"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13768..13770),aa:Ser,seq:gct)
     gene            complement(14121..14306)
                     /gene="csrA"
                     /locus_tag="IU325_13475"
     CDS             complement(14121..14306)
                     /gene="csrA"
                     /locus_tag="IU325_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HAP5810021.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(14541..17171)
                     /gene="alaS"
                     /locus_tag="IU325_13480"
     CDS             complement(14541..17171)
                     /gene="alaS"
                     /locus_tag="IU325_13480"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP5810022.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLNEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(17299..17799)
                     /gene="recX"
                     /locus_tag="IU325_13485"
     CDS             complement(17299..17799)
                     /gene="recX"
                     /locus_tag="IU325_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP5810023.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(17868..18929)
                     /gene="recA"
                     /locus_tag="IU325_13490"
     CDS             complement(17868..18929)
                     /gene="recA"
                     /locus_tag="IU325_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP5810024.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(19009..19506)
                     /gene="pncC"
                     /locus_tag="IU325_13495"
     CDS             complement(19009..19506)
                     /gene="pncC"
                     /locus_tag="IU325_13495"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HAP5810025.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(19651..20736)
                     /gene="mltB"
                     /locus_tag="IU325_13500"
     CDS             complement(19651..20736)
                     /gene="mltB"
                     /locus_tag="IU325_13500"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HAP5810026.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
BASE COUNT         4834 a         5625 c         5255 g         5109 t
ORIGIN      
        1 aaatatatcg aacaaggact ttcaaaagaa caaattctgg aaaaaactgg gctatcgctt
       61 ggcgtaaaag acgccagtct ggccattgaa gaaggcgaga tatttgtcat catgggatta
      121 tccggttcgg gtaaatccac aatggtacgc cttctcaatc gcctgattga acccacccgc
      181 gggcaagtgc tgattgatgg tgtggatatt gccaaaatat ccgacgccga actccgtgag
      241 gtgcgcagaa aaaagattgc gatggtcttc cagtcctttg ccttaatgcc gcatatgacc
      301 gtgctggaca atactgcgtt cggtatggaa ttggccggaa ttaatgccga agaacgccgg
      361 gaaaaagccc ttgatgcact gcgtcaggtc gggctggaaa attatgccca cagctacccg
      421 gatgaactct ctggcgggat gcgtcaacgt gtgggattag cccgcgcgtt agcgattaat
      481 ccggatatat tattaatgga cgaagccttc tcggcgctcg atccattaat tcgcaccgag
      541 atgcaggatg agctggtaaa attacaggcg aaacatcagc gcaccattgt ctttatttcc
      601 cacgatctcg atgaagctat gcgtattggc gaccgaattg ccattatgca aaatggtgaa
      661 gtggtacagg tcggcacacc ggatgaaatt ctcaataatc cggcgaatga ttatgtccgt
      721 accttcttcc gtggcgtcga tattagccag gtgttcagtg cgaaagatat tgcccgtcgt
      781 acaccgaacg gcttaattcg taaaaccccc ggcttcggcc cgcgttcggc actgaaatta
      841 ttgcaggatg aagatcgcga atatggctac gtcatcgaac gcggtaataa gtttgtcggc
      901 gctgtgtcta tcgattcgct taaagccgcg ttaacgcagc agcaaggtct tgatgcggcg
      961 ctgattgatg cgccgttagc ggtcgatgca caaacgcctc ttagcgagtt gctctctcat
     1021 gtcggacagg ccccctgtgc ggtgcccgtg gtcgacgagg accaacagta tgtcggcatc
     1081 atttcgaaag gaatgctgct gcgcgcttta gatcgtgagg gggtaaataa tggctgatca
     1141 aaataatccg tgggatacca cgccagcggc ggacagtgcc gcacagtccg cagacgcctg
     1201 gggtacaccg gcgactgcac cgactgacgg cggtggtgct gactggctga ccagtacgcc
     1261 agcgccaaac gtcgagcatt ttaatattct cgatccgttc cataaaacgc tgatcccgct
     1321 cgacagttgg gtcactgaag ggatcgactg ggtcgttacc catttccgtc ccgtcttcca
     1381 gggcgtgcgc gttccggttg attacatcct caacggtttc cagcaattgc tgctgggtat
     1441 gcccgctccg gtggcgatta tcgttttcgc tctcatcgcc tggcaaattt ccggtgtcgg
     1501 aatgggcgtg gcgacgctgg tttcgctgat tgccatcggc gcaatcggtg cctggtcaca
     1561 ggcaatggtt actctggcgc tggtgctaac cgccctgctg ttctgtatcg tcatcggttt
     1621 gccgttgggg atctggctgg cgagaagtcc acgagcggcg aaaattattc gtccactgct
     1681 tgatgccatg cagaccacgc cagcgtttgt ttatctggta ccaatcgtca tgctgtttgg
     1741 tatcggtaac gtgccgggcg tagtggtgac gatcatcttt gctctgccgc cgattatccg
     1801 tctgacaatt ctggggatta accaggttcc ggcggatctg attgaagcct cgcgctcatt
     1861 cggtgccagc ccgcgccaga tgctgttcaa agttcagtta ccgctggcga tgccgaccat
     1921 tatggcgggc gttaaccaga cgctgatgct ggccctttct atggtggtca tcgcctcgat
     1981 gattgcagtc ggcgggctgg gtcagatggt acttcgcggt atcggtcgtc tggatatggg
     2041 gcttgccacc gttggcggcg tcgggattgt gatcctcgcc attatcctcg accgcctgac
     2101 gcaggccgtt gggcgcgact cacgcagtcg cggcaaccgt cgctggtaca ccactggccc
     2161 tgttggtttg ctgacccgcc cattcattaa gtaactctgc acttgcccgg tgacgccggg
     2221 cattatcacc ctgccaaaaa aaggaataac aatgcgacat agcgtacttt ttgcgacagc
     2281 gtttgccacg cttatctcta cacaaacttt tgctgccgat ctgccgggca aaggcattac
     2341 tgttaatcca gttcagagca ccatcactga agaaaccttc cagacgctgc tggtcagtcg
     2401 tgcactggag aaattaggtt ataccgtcaa taaacccagc gaagtggatt acaacgttgg
     2461 ctacacctcg cttgcttccg gcgatgcaac cttcaccgcc gtgaactgga cgccgctgca
     2521 tgacaacatg tacgaagctg ccggtggcga taagaaattt tatcgtgaag gagtatttgt
     2581 taacggcgcg gcacagggtt acctgatcga taagaaaact gccgaccagt acaaaatcac
     2641 caacatcgcg caactgaaag atccgaagat cgccaaactg ttcgatacca acggcgacgg
     2701 taaagcggat ttgactggct gtaaccctgg ctggggctgc gaaggtgcga tcaaccacca
     2761 gcttgccgcg tatggactga ccaataccgt gacgcataat caggggaact acgcagcgat
     2821 gatggctgac accatcagtc gttacaaaga gggtaagccg gtgttttact acacctggac
     2881 gccgtactgg gtgagtaacg aactgaaacc ggggaaagat gtggtctggt tgcaggtgcc
     2941 gttctccgca ctgccgggcg ataaaaatgc cgataccaaa ctgccgaatg gcgcgaatta
     3001 cggcttcccg gtcagtacca tgcatatcgt tgccaacaaa gcctgggccg agaaaaaccc
     3061 ggcagcagcg aaactgtttg ccattatgca gttaccagtg gcagatatta acgcccagaa
     3121 cgccattatg catgacggca aagcctcaga aggcgatatt cagggccacg ttgatggctg
     3181 gatcaaagcc caccagcagc agttcgatgg ctgggtgaat gaggcgctgg cagcgcagaa
     3241 gtaaatattg ataaccgcgc agataatgct catgtgagta aaacaattgc agcgatggtt
     3301 ttaatccggc tgaaaatcga tgagacgttt tctgaaggcc ggggcgacgc gcagggatgc
     3361 gcggcgaggg cgcgtcttac agggatgtta cctcgcgccc gtacccgata gccgtaagaa
     3421 ataagtcgaa tgtaccgcgt agcggcgatt ttgtgccaaa agtttgggtc gccagggtgg
     3481 tggcgactga gccaccctgg cacgttcacc agcaaaatag tgacaaagta gctataatct
     3541 ggaagtgaac gtaacatttg ccttagtggc atatttcacc ttcaaatacc ctctccccat
     3601 tacccgcaca ttttaacaat ctatctttcg ccccatattc atcaacatcc gctattattg
     3661 atttccagct taatcatcac ctgatgaaca aaaataatga ctaaacctaa tcatgagctt
     3721 agcccggcac tgatcgtact gatgtctatc gccaccggtc tggcggtcgc cagcaactat
     3781 tacgcccagc cattgctcga caccattgcg cgtaactttt ccctttccgc cagttcggcg
     3841 ggctttattg ttaccgccgc gcagttgggc tatgccgcag gtctgctgtt tcttgttccc
     3901 ctcggtgata tgtttgaacg ccgccgcctg attgtctcga tgaccttact ggcagcgggc
     3961 ggtatgttaa tcactgccag cagtcagtca ctggcgatga tgatcctcgg tacggcatta
     4021 accggtttat tctcagtcgt ggcacaaatt ctggttccgc tggcagcgac gctggcttca
     4081 ccggataaac gcggcaaagt ggtcggcacc attatgagcg ggctgctgtt ggggatcttg
     4141 ctggcacgaa ccgttgccgg gttgctggca aacctcggcg gctggcgcac cgtcttttgg
     4201 gttgcctcgg tgttaatggc actgatggcg ctggcattat ggcgtggtct gccacaaatg
     4261 aaatcagaaa cccacctcaa ctacccacag ttgcttggtt ccgttttcag catgtttatc
     4321 agcgataaaa ttctgcgcac ccgcgcgttg ctgggctgcc tgacctttgc caacttcagc
     4381 attctctgga cctcaatggc ctttttgctt gccgctccac cttttaacta cagcgatggt
     4441 gtaattggtc tgtttggact tgcgggagct gccggagcgt tgggtgctcg tccggcgggc
     4501 ggttttgccg ataagggtaa atcgcaccac accactactt tcggtctgct gctgctatta
     4561 ctttcatggc tagcgatctg gtttggccac acttccgtac tggccttgat tatcggcatc
     4621 ctggtgctgg atctcaccgt gcagggcgtg catatcacta accagacggt aatttatcga
     4681 atacaccctg atgcgcgtaa tcgcctgacc gcaggttaca tgaccagcta ctttattggc
     4741 ggtgccgccg gttcgctaat ttcagcctca gcctggcaac atggcggttg ggctggcgtt
     4801 tgtctggctg gcgcgacgat tgccctggtt aacttactgg tctggtggcg aggttttcat
     4861 cgtcaggaag ctgcaaatta agcaatcagc aaccgttttc gtggctttac acttataagg
     4921 gtgttaagaa gcccatcagt ctgataaggt taagatattc attcagtcta tttataatat
     4981 taacaatcgt taagcgtaca ctctatggaa agccctactc cacagcctgc tcctggttcg
     5041 gcgaccttca tggaaggatg caaagacagt ttaccgattg ttattagtta tattccggtg
     5101 gcctttgcgt tcggtctgaa tgcgacccgt ctgggattct ctcctctcga aagcgttttt
     5161 ttctcctgca tcatttatgc aggcgcgagc cagttcgtca ttaccgcgat gctggcagcc
     5221 gggagtagtt tgtgggttgc tgcactgacc gtcatggcaa tggatgttcg ccatgttttg
     5281 tatggcccgt cactgcgtag ccgtattatt cagcgtctgc aaaaatcgaa aaccgccctg
     5341 tgggcatttg gcctgacgga tgaggttttt gccgccgcaa ccgcaaaact ggtacgcaat
     5401 aatcgccgct ggagcgagaa ctggatgatc ggcattgcct tcagttcatg gtcatcgtgg
     5461 gtatttggta cggtaatagg ggcattctcc ggcagcggct tgctgcaagg atatcccgcc
     5521 gttgaagcag cattaggttt tatgcttccg gcactcttta tgagtttcct gctcgcctct
     5581 ttccagcgca aacaatctct ttgcgttacc gcagcgttag ttggtgccct tgcaggcgta
     5641 acgctatttt ctattcccgt cgccattctg gcaggcattg tctgtggctg cctcactgcg
     5701 ttaatccagg cattctggca aggagcgccc gatgagctat gaggttctgc tgcttgggtt
     5761 actggttggc gcggcgaatt attgcttccg ctatttgccg ctgcgcctgc gtgtgggtaa
     5821 tgcccgccca accaaacgtg gcgcgatagg catattgctc gacaccattg gcatcgcctc
     5881 gatatgcgct ctgctggttg tctctaccgc accagaagtg atgcacgata cacgccgttt
     5941 cgtgcccacg ctggtcggct tcgcggtact gggtgccagt ttctataaaa cacgcagcat
     6001 tatcatccca acactgctta gtgcgctggc ctacgggctc gcctggaaag tgatggcgat
     6061 tatataaccc acaagaatca tttttctaaa acaatacatt tactttattt gtcactgtcg
     6121 ttactatatc ggctgaaatt aatgaggtca tacccaaatg gatagttcgt ttacgcccat
     6181 tgaacaaatg ctaaaatttc gcgccagccg ccacgaagat tttccttatc aggagatcct
     6241 tctgacccgt ctttgcatgc acatgcaaag caagctgctg gagaaccgca ataaaatgct
     6301 gaaggctcag gggattaacg agacgttgtt tatggcgttg attacgctgg agtctcagga
     6361 aaaccacagt attcagcctt ctgaattgag ttgtgctctt ggatcatccc gtaccaacgc
     6421 gacgcgtatt gccgatgaac tggaaaaacg cggttggatt gaacgtcgtg aaagcgataa
     6481 cgatcgccgc tgcctgcatc tgcaattaac ggaaaaaggt cacgagtttt tgcgcgaggt
     6541 tttaccaccg cagcataact gcctgcatca actctggtcc gcgctcagca caacagaaaa
     6601 agatcagctc gagcaaatca cccgcaaatt gctctcccgt ctcgaccaga tggaacaaga
     6661 cggtgtggtt ctcgaagcga tgagctaacg cgtcatctcg ctcaaaaatc cagatttata
     6721 aaagaaaaaa tgactggcca gcatcgcaac atgctggcct ttttggcaag caggtcggct
     6781 cagccgatga gttaagaaga tcgtggagaa caatatgagc gcaaatgcgg agactcaaac
     6841 cccgcagcaa ccggtaaaga agagcggcaa acgtaagcgt ctgctcctcc ttctcacctt
     6901 gctctttata attattgccg tagcgatagg gatttattgg tttttggtac tgcgtcattt
     6961 cgaagaaacc gatgacgcat acgtggcagg gaatcagatt caaattatgt ctcaggtgtc
     7021 tggcagcgtg acgaaagtct gggccgataa caccgacttt gtaaaagaag gcgatgtgct
     7081 ggtcaccctc gacccgacag atgctcgcca ggcgtttgaa aaagccaaaa ctgcactggc
     7141 ttccagcgtt cgccaaaccc accagctgat gattaacagc aaacagttgc aggcgaatat
     7201 tgaggtgcaa aaaatcgcac tcgcgaaagc acaaagcgac tacaaccgcc gtgtgccgct
     7261 gggcaatgcc aacctgattg gtcgcgaaga gctgcaacac gcccgcgacg ccgttaccag
     7321 tgcccaggca caactggacg tcgcgattca acaatacaat gccaatcagg cgatgattct
     7381 ggggactaaa ctggaagatc agcctgccgt gcaacaggct gccaccgaag tacgtaacgc
     7441 ctggctggcg ctggagcgta ctcgtattgt cagtccgatg accggttatg tctcccgccg
     7501 cgcggtacag cctggggcac aaattagccc aacgacgccg ctgatggcgg tcgttccagc
     7561 caccaatatg tgggtggatg ccaactttaa agagacgcag attgccaata tgcgcatcgg
     7621 tcagccggtc accatcacca ccgatattta cggcgatgat gtgaaataca ccggtaaagt
     7681 ggttggtctg gatatgggga caggtagcgc gttctcactg cttccggcgc aaaatgcgac
     7741 tggtaactgg atcaaagtcg ttcagcgtct gcctgttcgt attgaactgg accagaaaca
     7801 gctcgagcaa tacccgctgc gtatcggttt gtccacgctg gtgagcgtca ataccactaa
     7861 ccgtgacggt caggtactgg caaataaagt acgttccaca ccggtagcgg taagcaccgc
     7921 gcgtgaaatc agcctggcac ctgtcaataa actgatcgac gatatcgtaa aagctaacgc
     7981 tggctaatcc agaggtgcgt gtgatgcaac agcaaaaacc gctggaaggc gcgcaactgg
     8041 tcattatgac gattgcgctg tcactggcga cattcatgca ggtgctggac tccaccattg
     8101 ctaacgtggc gatccccact atcgccggga atctgggctc atcgctcagc cagggaacgt
     8161 gggtaatcac ttctttcggg gtggcgaatg ccatctcgat cccgcttacc ggctggctgg
     8221 caaagcgcgt cggggaagtg aaactgttcc tctggtccac catcgccttt gctattgcgt
     8281 cgtgggcgtg tggtgtctcc agcagcctga atatgctgat tttcttccgc gtgattcagg
     8341 ggattgtcgc cgggccgtta attccgcttt cgcaaagtct attgctgaat aactacccgc
     8401 cagccaaacg ctcgatcgcg ctggcgttgt ggtcgatgac ggtgattgtt gcgccaattt
     8461 gcggcccgat cctcggcggg tatatcagtg acaattacca ctggggctgg atattcttca
     8521 tcaacgtacc gattggcgtg gcggtggtgt tgatgacact gcaaaccctg cgcggacgtg
     8581 aaacccgcac cgaacggcgg cggattgatg ccgtggggct ggcactgctg gttattggta
     8641 tcggtagcct gcagattatg ctcgaccgcg gtaaagagct ggactggttt tcatcacagg
     8701 aaattatcat ccttaccgtg gtggcagtgg tggctatctg cttcctgatt gtctgggagc
     8761 tgaccgacga taacccgata gtcgatctgt cgttgtttaa gtcgcgcaac ttcaccatcg
     8821 gctgtttgtg tatcagcctc gcgtatatgc tctacttcgg cgctattgtt ctgctgccgc
     8881 agttgttgca ggaggtctac ggttacacgg caacgtgggc aggtctggcc tctgcgccgg
     8941 taggaattat tccggtgatc ctgtcgccga ttatcggccg cttcgcgcat aaactggata
     9001 tgcggcggct ggtaaccttc agctttatta tgtatgccgt ctgcttctac tggcgtgcct
     9061 atacctttga accgggtatg gattttggcg cgtcggcctg gccgcagttt atccaggggt
     9121 ttgcggtggc ctgcttcttt atgccgctga ccaccattac gctgtctggt ttgccaccgg
     9181 aacgactggc ggcggcatcg agcctctcca actttacgcg tacgctggcg gggtctatcg
     9241 gcacgtcgat aaccacaacc atgtggacca accgcgagtc gatgcaccat gcgcagttaa
     9301 ccgagtcggt aaacccgttt aacccaaacg cccaggcgat gtacagtcaa ctggaaggac
     9361 tgggaatgac gcaacagcag gcatcaggct ggattgccca gcagatcacc aatcaggggc
     9421 tgattatttc cgccaatgag atcttctgga tgtcagccgg gatattcctc gtcttgctgg
     9481 ggctggtgtg gtttgctaaa ccgccatttg gcgcaggtgg cggcggaggt ggtgcgcact
     9541 aagtacaact aagccagttc atttgaactg gcttttttca attaattgtg aagatagttt
     9601 actgactaga tgtgcagttc ctgcaacttc tctttcggca gcgccagttc ttcgttgctg
     9661 ttgatgcgca cgtcacgttc cagaatgcta cgcgcaatat cctgcgcttc ctgcaacgag
     9721 tgcatctggt aagtgccaca ctggtagacg ttcagttccg ggatctgatt ctgatcctgc
     9781 actttcagca cgtcttccat tgccgctttc caggcatcag caacacgctg ctcatctggc
     9841 gtaccaatca gactcatata aaaaccggtg cggcagccca ttggcgagat atcgataatc
     9901 tctacaccat taccgttaag atggttacgc ataaaaccag caaacaggtg ctccagggta
     9961 tggatccctc tttctggcat cacttctttg ttcggcacgc agaagcgcag atcgaacacg
    10021 gtgattgcgt cgccatgcgg ggtgttcatt gttttcgcca cccgaactgc aggcgcttcc
    10081 atccgggtat gatcgactgt gaagctatct aacaacggca tttagccacc tccggtaatt
    10141 tttttaaaaa ttttctgaac tctttcttcc caggcgagtc tgagtatatg aaagacgcgc
    10201 atttgttatc atcatccctg aattcagaga tgaaattttg gccactcacg agtggccttt
    10261 ttcttttctg tcaggcgtgt ttttccagcc acaccgcaaa cggttcggta tcagcggctt
    10321 ccatttcctg ctgacggcgt tcagacgcct cgcgctcggc tacaaaatcc tcttcgcgca
    10381 gaatttccaa cggctcttca cgcagcagat tacggtacgc ttcagcaaac gctttgcctg
    10441 ttccgccaat accagtatca atcatagacc ttaagatacg ggcagagaaa gtcagatcgg
    10501 gattatcgaa gcaggcgacc agttcatcac acactttctg atacgcttcg ccgccgttaa
    10561 tgctatccag cgtttgcgca acgcgtttca gatcgcggaa cagatcttta cccacctgcg
    10621 gtaacgggaa ctgcgcggtt tcgcagccga tacccagcgt caggcccggt ttgcgacctt
    10681 cgaggatcac ccggttccag ttaacgcgtg tacaggcaag ttctttactg ctcatttccg
    10741 gtgcatcagc cagcgcacac cagaccataa acaggtcgag gaatcgcacc tgctgttcat
    10801 ctacaccaat cggcgagaat gggttgatgt ccagcgaacg cacttcaata tattcaatgc
    10861 cgccacgtaa cagcgcatca gaaggcgact cgccgctgcg ggtaacgcgt tttggacgaa
    10921 tcggcgcgta cagttcgttt tcaatctgca gcacgttgct gttgatttgc agcctcttac
    10981 catctttctc aatgccaatc ttggcgtact cttccgatgg cgttttgatt gcctgtttaa
    11041 ggcccgctac gtactcgtaa agatcgttga aggtaatacc tagattgctt tgcgatttat
    11101 tggtatagcc gagatcactc aaacgaagag aggtcgcata cggcaggtaa tacataccgc
    11161 actcggtttt ctcaaacggc agcgatgttg gttttccttg caggaaagaa gaacaaatcg
    11221 ccggagacgc gccaaacaga taaggaatga cccaaccgaa acgatagtaa ttgcggataa
    11281 cgcggaaata gcccgcagaa attttctctt tggcatcagc gcccgcgata tcaccacact
    11341 tcgcttgcca gaatgccatt ggcaaagaga aattgtagtg cacgccggaa atggtttgca
    11401 tcagcgcgcc gtagcgattt ttcagccctt cacggtacag cgttttaaag cgtccggtgt
    11461 tagaagtgcc gtactgtgcc agttcgatgt cctgaccttc tgcgatgtag catggcatac
    11521 ttaacggcca catccgctca tcgcccatat tgcgcgccgt ataacgatgc agatcgcgca
    11581 taaaggtcag catatgttca atatcaccat ccactggtgt aatgaattcc agcaatgctt
    11641 ccgcaaaatc ggtagtaatc catttgtgcg tcagtgcgga acctaatgct tcaggatgac
    11701 ctgttgttgc cagtgtgcca tcagcattaa cacgcaaagt ttcgcgctcc agcccacgct
    11761 gtatcccctt taacgcctga ggatgttttt ccagccaggc cagcgcctgt gatacgtccg
    11821 ggatcaaatt gacctcccgc ctgtcaaaat cgttttaatt agcataactg taatggtgac
    11881 catatgtgca ggcctacaat tagtgccacc acatcatgcc ctgaacggtc gctgctgcaa
    11941 ctgcaacata gcgtaacgct ttaccaaggc acaaaaaaaa gattaccggc ccccaggaga
    12001 tgcgcatcca tcccgctaac agacacagta aatcgccaac cacgggcatc cagcttaata
    12061 atagcgtgac tgcaccatag cgtttcagcc agccggtagc tttctcttgc cagcgcgatg
    12121 ttttacgcaa tggaaagaaa cgcccaagga taacgttagt taaccctcca aggctattac
    12181 ccattgttgc tgttaaaact aaaacccagg gatgactgat cccggaaagc aacattgcca
    12241 ccagcacgac ttcggagttg ccgggtaata gtgtagcgct gagaaaacta ctggcaaaca
    12301 acgagaaaag cgataacgct tcactcacag caagcgaaca tccacagcgt ccatgcctgc
    12361 tgcacgggcc gcctgaatac cgaaatcggc atcttcaaag accacacact gtgtcggttg
    12421 cacgcccata cgctgcgcgc acaacaaaaa tgtgtctggc gcgggtttat ggtgtttgac
    12481 gtgatcagca gcgacgacgg cgtcaaaata acggcgtaat cccaggtgcg ccagcaatgc
    12541 ctcagcgatg gcgctttcac tccccgttcc tacagccatt gggcgacgac catgccagct
    12601 tttcaccact tcaacaagag gaagcggctc gacactatcc agcagcatac ttcttactgc
    12661 ttctgttttt tcacgcgcta acgcatgcgg gtcgagatcg gcctgattca gctcaataat
    12721 cgcctgagca atacgccagg tgggcgagcc attaagcgca atcatcgcct gaacatcgta
    12781 ctgaagaccg tagtgcccta atacttcgcg ccacgcttta cggtgcgtag gctcggtatc
    12841 caggatggtg ccatccatat caaaaattaa acctgcataa cgctcgtaca tggtcttctc
    12901 gcaagtcgaa aaatatgacg ctactttagc gtaattgctt gattttgtcg ctgatggcga
    12961 aggagaagaa aattcaggaa atggataaag tagttattta gaaatgagat atttttgaag
    13021 gaaatttttt ggagaagatg gtgcatccgg gaggattcga acctccgacc gctcggttcg
    13081 tagccgagta ctctatccag ctgagctacg gatgcatcgg gaaaacttat tttactgctg
    13141 atattgatac cgctactaaa gccatatcaa ataagaaaat ccatcttaac catcatcact
    13201 gatgattacg gagaatatgg tgcatccggg aggattcgaa cctccgaccg ctcggttcgt
    13261 agccgagtac tctatccagc tgagctacgg atgcatcggg aaatttattt tactgctgat
    13321 attgataccg ctgctaaagc catatcaagt aagagatggt gcatccggga ggattcgaac
    13381 ctccgaccgc tcggttcgta gccgagtact ctatccagct gagctacgga tgcatcggga
    13441 aacttacttt actgcagatt ttttgatacc gctactaaag ccgtatcaag taagagatgg
    13501 tgcatccggg aggattactc gccttgcggc tcgcccttcg ggccgttgct aaagcaacgt
    13561 tatcctccct ggtgcctgcg gttattaccg ccacctcttc cataccttca tcgctgaaag
    13621 tacgtagaat atggtgcatc cgggaggatt cgaacctccg accgctcggt tcgtagccga
    13681 gtactctatc cagctgagct acggatgcaa atggcggtga ggcggggatt cgaaccccgg
    13741 atgcagcttt tgaccgcata ctcccttagc aggggagcgc cttcagcctc tcggccacct
    13801 caccacacgc ctcttacgag tgcttcgaag aacttgttta tcgctcatcg tcgctgcgtg
    13861 gcgcacatat tactttctga gacttataag tcaaacaatt tttcccacac ttttatcgtt
    13921 tgcacacttc acgctcaatt agtctgtaaa aacgtcaaaa agagtgtttt atcaacagaa
    13981 aaacggaggt ctgtcagata gcagtaatgc aaaaaaatgg agacttaagt tgaatgaacg
    14041 ggagtaaagc gaaaagacaa tggagtgaag gagatatttt gaggggtgcg tctcaccgat
    14101 aaagatgaga cgcggaaaga ttagtaactg gactgctggg atttttcagc ctggatacgc
    14161 tggtagatct cttcacggtg aacagaaact tccttcgggg catttacgcc aatacgtacc
    14221 tggttgccct ttacccctaa aactgtcacg gtgacctcat ccccaatcat gagggtctca
    14281 ccaactcgac gagtcagaat cagcattctt tgctccttga aagattaaaa gagtcgggtc
    14341 tctctgtatc ccggcattat ccatcatata acgccaaaaa gtaagcaatg acaaacacat
    14401 tacatgtaag cagtcatggc attacattct gttaaaccta agtttagccg atatacacaa
    14461 cttcaacctg actttatcgt tgtcgatagc gttaatgcga atgccgtgaa gcgagtccac
    14521 ggcattgcct gacgcttata ttattgcaat ttcgcgctga cccagccttt cacactggct
    14581 aacgctgcag gtaaggccgc agcatccgta ccaccggctt gcgccatgtc aggacgtcca
    14641 ccacccttgc cgcccacctg ctgagcgacc ataccaatca gttcccctgc tttcacacga
    14701 tctgtgacgt ccttagatac gcctgcaatc agagaaacct taccttcggc taccgttgcc
    14761 agcacgataa ttgtcgaccc cagctgattt tttaaatcgt caaccatggt acgcaacatt
    14821 ttcggctcaa caccgctaag ctcgctaacc aacagcttaa caccattaac atcaattgcc
    14881 ttactggaaa gatttgcgct ctcctgtgcg gcagcttgtt ctttaagctg ttgtaactct
    14941 ttttccagct gacgcgtacg ttccagtact gagcgcactt tatcagccag attattgcta
    15001 tcgcctttca gcagatgcgc gacttcgttt aagcgatcgc tgtctgcatg aacggtggcg
    15061 atagcgcctt ctccggttac cgcttcgata cgacgaacgc ctgcagcagt acccgattca
    15121 gagatgatgc ggaacagacc aatatcacca gtgcggctgg cgtgagtacc gccacacagc
    15181 tcggtggaga aatcgcccat gctcagcacg cgtacgcgct catcatactt ctcgccgaac
    15241 agcgccatcg cacctttcgc tttcgccgct tcgagatcca tgatgttggt ttcgatcggc
    15301 aagttacggc gaatctgtgc gttcaccagg tcttcgaccg cacgaatctc ttctggtttc
    15361 atcgcttcgt tgtgtgagaa gtcgaagcgc agcactttgt cgttaaccag tgaacctttc
    15421 tgcgatacat gagtacccag aacctggcgc agcgcagcgt gcatcaggtg cgttgcggag
    15481 tgattcagac gaatacgggc gcgacgagcc tcatcaacat cagcctgcac cgcgtcgccc
    15541 actttcagag aacccgcagc aagtttaccg atgtgaccaa tcgcctggcc gtatttctgc
    15601 gtatcttcca ccgcaaagga gaagttagcg cctttcagtt cgcctttatc accaacctga
    15661 ccgccggatt ccgcatagaa tggcgtttga tccagcacga ccacagcttc ctggcctgca
    15721 ttgatggcat caaccgcttt accatcaaca aacagcgcgg tcactttgcc gttcagttcc
    15781 agatggtcat agcctttaaa ttcagatgcg ctgtcaacac ggatcattgc gttgtaatcg
    15841 gcaccaaagc cgctggcttc gcgcgcgcga cgacgctgct cttccattgc tgcttcgaaa
    15901 ccagcttcgt caactttgat gttgcgctca cgacaaacat cagccgtcag gtcaaccggg
    15961 aagccatagg tgtcgtacag acggaaagcc gtttcgccat ccagcgtatc accagaaagt
    16021 tttgccagct cttcatccag cagcgccaga ccgcgctcca gagtacgggc aaactgctct
    16081 tcttcagtct tcagcacctg ctcaacctgc gcctgctggc gtttcaggtc ttcacccgca
    16141 gagcccatta cgtcgatcag cggaccaacc agtttgtaga agaaggtttc tttcgcgccc
    16201 agcatattgc cgtgacgcac tgcgcgacga atgatacgac gcagtacata accacggttt
    16261 tcattggacg gcattacgcc atccgcgatc aggaacgcac aagaacgaat gtggtcagcg
    16321 attacgcgca gcgatttatt gctcagatcg gtcgcgccag tgactttcgc taccgcctgg
    16381 atcagcgtgc ggaacaggtc gatgtcatag ttagagttaa cgtgttgcag caccgcagca
    16441 atacgctcca gacccatacc ggtatctaca gacggcttcg gcagcggttc catcgtgcca
    16501 tctgcctggc ggttgaactg catgaagacg atgttccaga tctcaatgta gcgatcgccg
    16561 tcttcttccg gacttcccgg agggccaccc caaatgtggt cgccgtgatc gtagaagatt
    16621 tcggtgcacg ggccgcacgg accagtgtca cccatctgcc agaagttgtc agatgcgtat
    16681 ggcgcacctt tgttatcgcc gatgcgaata atacgttcgc gcgggatccc tacttctttt
    16741 tcccagattt cgtaggcttc gtcgtcgctt tcatagacgg taacccacag acgctctttc
    16801 ggcagggcaa accatttttc actggtcagc agttcccatg caaactgaat ggcatcgtgt
    16861 ttgaaatagt cgccgaagct gaagttgccc agcatttcga agaaggtatg atgacgcgcg
    16921 gtgtaaccga cgttttccag gtcgttgtgt ttaccacccg cacgcacgca gcgttgggaa
    16981 gtggtagcgc gggaataatt acgcttgtcg agcccaagga acacatcctt gaactggttc
    17041 atcccggcgt tggtaaacaa caaagttggg tcgttatggg gtaccaggga gctgctggca
    17101 actacctgat gtcccttact atggaaaaag tcgagaaacg cctgacggat ctcagcggtg
    17161 ctcttgctca taattatcct gaaatcaagc taacgaaata tcgccaccag ctccagcgtg
    17221 tcttaaccgc cgggctggta actgaaaagt gggaataaga taagttttct tgactgggaa
    17281 gtaaaatacc gtatgcgttc agtcggcaaa atttcgccaa atatcctgga tatcttccat
    17341 cagatagcca cgatagagca gaaaacgctg gatcttaact ttttctgaaa agacagttgg
    17401 caaaggttcg ccatattttc gcgtcgcctg atcgcgcgcc agtgcgcacc agtcgatgtc
    17461 acattcacgc atcgcttttt ctgtcgcttc gcgggaaata cctttctgat tcagttcctg
    17521 acgaatacgc gcaggtccat aacctttgcg gctacggctg gcgataaagc gcgcaacaaa
    17581 tcggctgtca tcgagatagc catgttcatg gcaccaggcg ataacgcgct cgtaatcttc
    17641 tgccgtggca tcaatctctt ccgggccatt tttgcccata atcggtgccg cgagtttacg
    17701 tcgcagttct tgctcactgt gatcgcgcac cgccagaatg cgtaccgcac gatccaacag
    17761 gcgagcatat gccgggcgac gggacgttga ttctgtcatg gcatatcctt acaacttaaa
    17821 aaagcaaaag ggccgcagat gcgacccttg tgtatcaaac aagacgatta aaaatcttcg
    17881 ttagtttctg ctacgccttc gctgtcatct acagagaaat ccggcgttga gttcgggttg
    17941 ctcagcagca actcacgtac tttcttctcg atctctttcg cggtttccgg gttatctttc
    18001 agccaggcag tcgcattcgc tttaccctga ccgatcttct cacctttgta gctgtaccac
    18061 gcacctgctt tctcgatcag cttctctttc acgcccagat caaccagttc gccatagaag
    18121 ttgatacctt cgccgtagag gatctggaat tcagcctgtt taaacggcgc agcgattttg
    18181 ttcttcacca ctttcacacg ggtttcgcta cccaccacgt tttcgccctc tttcaccgcg
    18241 ccgatacgac ggatgtcgag acgaacagag gcgtagaatt tcagcgcgtt accaccggta
    18301 gtggtttccg ggttaccgaa catcacacca attttcatac ggatctggtt gatgaagatc
    18361 agcagcgtgt tggactgctt caggttaccc gccagcttac gcatcgcctg gctcatcata
    18421 cgtgccgcaa ggcccatgtg agagtcgccg atttcgcctt cgatttccgc tttcggcgtc
    18481 agtgccgcca cggagtcaac gacgataacg tctactgcac cagaacgcgc cagggcgtca
    18541 cagatttcca gtgcctgctc gccagtgtcc ggctgggagc acagcaggtt gtcgatatcg
    18601 acgcccagtt tacgtgcata gattgggtcc agcgcgtgtt cagcatcgat aaacgcacag
    18661 gttttacctt cacgctgcgc tgcggcgatc acctgcaacg tcagcgtggt tttaccggaa
    18721 gattccggtc cgtagatttc gacgatacgg cccatcggca gaccacctgc cccaagcgcg
    18781 atatccagtg aaagcgaacc ggtagagatg gtttccacat ccatggaacg gtcttcaccc
    18841 aggcgcatga tggagccttt accaaattgt ttctcaatct ggcccagtgc tgccgccaac
    18901 gctttctgtt tgttttcgtc gatagccatt tttactcctg tcatgccggg taataccgga
    18961 tagtcaatat gttctgttga agcaattata ctgtatgctc atacagtatc aagtgttttg
    19021 tagaaattgt tgccacaagg tctgcaatgc atacgcagta gcctgacgac gcaccgcatc
    19081 acggtcaccg ctgaagcatt cccgccgggt aataccttcg ccgcgggcgg tggcaaaagc
    19141 aaaccagacg gtgccgacag gcttctcttc actgccgcca tccggcccgg cgataccact
    19201 aatagacaca gcataatcag cacgagccgc tttcagtgcg cctatcgcca tttccaccac
    19261 gacaggttcg ctcaccgcgc catgctgcgc cagcgtctct tcgcgtacac cgatcatttg
    19321 cgctttcgct tcgttactgt aggtgacaaa tccgcgttca aaccaggcgg aactgccggc
    19381 aatatcggta atcactttcg ctacccaacc accggtacaa gactcagcag ttgttacggt
    19441 tgcgccacgg gctttcagcg cctgcccaac ctgttcgctt aactgcatca gttcactgtc
    19501 agtcatcaca acctctgtca gtcaaaaatt tatgccggac aagatagcac tttcgtcagg
    19561 aagatggtgt tggcgttgca attttacgag gaggattcag aaaaaagctg attagccaga
    19621 gggaagctca cgctcccctc ttgcaaatag ttactgcacg cgcgccagcg ccacggcttg
    19681 tcctaactgc cagaccgcca ttgcgtaatg ggtactgtgg ttgtaacggg tgatggtgta
    19741 gaagttcggc agaccgtacc agtactggta accggtaccg acatccagac gcagcaggct
    19801 ggcttgttga tggttgccca gcggctgctg tggcgttaaa cctgccgcgg caagctgcga
    19861 aatgctgtac ttggttttga agccatttgg caagcctgga gcctgaccgt ttgccatcac
    19921 cgcaacctga tcacctttca cccagccgtg cgctttgaaa tagttcgcca cactaccaat
    19981 ggcatcaacc ggatcccaca ggttgatatg cccgtcgccg ctgaaatcta ccgcgtattg
    20041 tttgtaagac gacggcataa actgtccgta gcccatcgcc ccggcaaagg aacctttcag
    20101 attgagcgga tcgtcctgct cgtcgcgcgc catcagcagg aaagtttcca gctcgccaga
    20161 gaaatactcc gcgcggcgtg ggtagttaaa tgacagcgtt gccagcgcat cgaggatgcg
    20221 agttttcccc atcacgcgcc cccagcgcgt ttcaacgccg ataatcccga cgataatttc
    20281 cggcggtacg ccatacacct gccacgcgcg attcagcgca tcttcatact gattccagaa
    20341 aaccacaccg ttctgcacgt tgtccggcgt aataaatttt ttgcgataac gcagccatgc
    20401 gccgttcgga cctgatggcg gtttcaccga tgtggttggt gcctggttat ccatcagccg
    20461 cagtaccgaa tccagacgtt tcgcctggga gagaatttcc tgcaactgct gacgatcgaa
    20521 accgtgtttg ttcaccattt tgtcgatgaa ctgctgggca ttcgggttat tagcgaaatc
    20581 gccgcccatc tgcatcacat tgtgctgcgg ctcaagcagg aagccgccag acggcgttcc
    20641 ggtggtcgtt tcagtctcag taggttttgg cttgctgcta caggcggcaa gcaacacaaa
    20701 aaggggaagc aatgttacat aacgacgctt gaacatgagg ggtccattta acagattcaa
    20761 ccaggggcaa gtatggtaaa gcatcacgcc ccgcacaagg aagcggtagt cactgcccga
    20821 tac
//
LOCUS       DACBVI010000076        20374 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.076, whole genome shotgun sequence.
ACCESSION   DACBVI010000076 DACBVI010000000
VERSION     DACBVI010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.076"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            102..800
                     /gene="yeiL"
                     /locus_tag="IU325_13505"
     CDS             102..800
                     /gene="yeiL"
                     /locus_tag="IU325_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HAP5810027.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(868..2118)
                     /locus_tag="IU325_13510"
     CDS             complement(868..2118)
                     /locus_tag="IU325_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP5810028.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKGISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFTNASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(2212..3150)
                     /locus_tag="IU325_13515"
     CDS             complement(2212..3150)
                     /locus_tag="IU325_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HAP5810029.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSATEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(3138..4079)
                     /locus_tag="IU325_13520"
     CDS             complement(3138..4079)
                     /locus_tag="IU325_13520"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HAP5810030.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHGEFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSVPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(4503..6194)
                     /gene="fruA"
                     /locus_tag="IU325_13525"
     CDS             complement(4503..6194)
                     /gene="fruA"
                     /locus_tag="IU325_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HAP5810031.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKTQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(6211..7149)
                     /gene="fruK"
                     /locus_tag="IU325_13530"
     CDS             complement(6211..7149)
                     /gene="fruK"
                     /locus_tag="IU325_13530"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP5810032.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(7149..8279)
                     /locus_tag="IU325_13535"
     CDS             complement(7149..8279)
                     /locus_tag="IU325_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HAP5810033.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            8647..9828
                     /gene="setB"
                     /locus_tag="IU325_13540"
     CDS             8647..9828
                     /gene="setB"
                     /locus_tag="IU325_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HAP5810034.1"
                     /translation="MHNSPAVSSAKSFDLTSMAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(9825..10079)
                     /locus_tag="IU325_13545"
     CDS             complement(9825..10079)
                     /locus_tag="IU325_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HAP5810035.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            10234..10806
                     /gene="yeiP"
                     /locus_tag="IU325_13550"
     CDS             10234..10806
                     /gene="yeiP"
                     /locus_tag="IU325_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HAP5810036.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            11029..12495
                     /locus_tag="IU325_13555"
     CDS             11029..12495
                     /locus_tag="IU325_13555"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HAP5810037.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQNPEEPHS
                     APGILVEALKRRRERGLAPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLARE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIAQFGAHQALLNTLKI"
     gene            12613..13599
                     /locus_tag="IU325_13560"
     CDS             12613..13599
                     /locus_tag="IU325_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810038.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNERWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKIDGHLLDLPRRNLAELPASAAHSHHHSVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVMRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            13638..14351
                     /gene="lpxT"
                     /locus_tag="IU325_13565"
     CDS             13638..14351
                     /gene="lpxT"
                     /locus_tag="IU325_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HAP5810039.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTNINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            14763..15329
                     /gene="mepS"
                     /locus_tag="IU325_13570"
     CDS             14763..15329
                     /gene="mepS"
                     /locus_tag="IU325_13570"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HAP5810040.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            15510..17066
                     /locus_tag="IU325_13575"
     CDS             15510..17066
                     /locus_tag="IU325_13575"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HAP5810041.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQSLRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            17148..18962
                     /locus_tag="IU325_13580"
     CDS             17148..18962
                     /locus_tag="IU325_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="with YejBEF is involved in resistance to microcin
                     C; Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP5810042.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPLARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKNHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPSSSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            18963..20057
                     /gene="yejB"
                     /locus_tag="IU325_13585"
     CDS             18963..20057
                     /gene="yejB"
                     /locus_tag="IU325_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HAP5810043.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            20057..>20374
                     /locus_tag="IU325_13590"
     CDS             20057..>20374
                     /locus_tag="IU325_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416684.1"
                     /note="part of the microcin C transport system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP5810044.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLEDKGWVLWAPIRFGA
                     TSIN"
BASE COUNT         5010 a         5265 c         4930 g         5169 t
ORIGIN      
        1 tattttttct acagcaaaga aaattcgacc gggccgatga ttgaatctta acaacagcgt
       61 aagtatgcta aatatgagaa atctcatagc ggataaacat cgtgaaagaa atccacaata
      121 atgaccttaa gcagcaattg atgagtgaat ccgcgtttaa ggattgcttt tcaacggatg
      181 tttcagccga tacgcggctg tttcattttt tagcgcgtga ctacattgtg caggaagggc
      241 aacagccgtc ctggctgttt tacctgacgc gaggccgcgc caggctttac gccacgctag
      301 ctaatggtcg cgtgtcgctg atcgatttct ttgccgctcc ctgttttatt ggcgagattg
      361 agttaatcga caaagaccat gaaccgcgtg cggtacaggc tattgaagag tgttggtgcc
      421 ttgcgctccc tatgaaacat taccgtccgc tgttattaaa cgacacgcta tttttacgaa
      481 aactctgcgt caccttaagc cataaaaatt atcgcaatat tgtttcttta actcagaatc
      541 aatcatttcc gttagtgaat cgcctggcag cttttatatt actctcgcag gaaggtgatc
      601 tttatcacga aaagcatacg caagcggcag agtatttagg cgtttcttat cgacatcttt
      661 tatatgttct agcgcaattc attcacgacg gtttattaat taaaagcaag aaagggtatc
      721 taattaaaaa cagaaagcag ttgtcaggac tggcactgga gatggacccg gaaaataaat
      781 tctcagggat gatgcagtaa aaattatttg caatagagcg attgccggat gtgacgctaa
      841 ataagcgcgt acatccggca atgaaactca agctaaacca ataaagaacc cggcaatggt
      901 cgcgctcatc aggttggaaa gcgttgctgc tgccagcgcc cgtaaaccaa gctgggcgat
      961 ttccggtgcg cgtttaggcg aaatagccga aaatgcgcca acgacaacac cgatagaacc
     1021 aaagttagca aaaccacaaa gcgcaaagga gataatcgca atggttttca cttccagcgt
     1081 gccgcccgtt tgcaggtatg gggagaaact caggtaagcg acgaattcgt taatcgccag
     1141 tttctgccca atcaggctac ccgcaagatt ggcatcactc cagtccacac ccatgatcca
     1201 cgccagcggt gccagcacat agccaaaaat actttccaga gaagcattgg taaaaccaaa
     1261 ccagccgcca attccgccga taatgccatt aatcagcgca ataattgcga caaacgccat
     1321 taccaccgtc gccacaccag cggcgatttt caacccggtc atcgcaccgc tcgccgctgc
     1381 ttcgataaag ctttttggcg gtgtttcgct gaacgacaga ttttcaaatg tgacctgcga
     1441 aggctcggtt gccgggctaa gaatacgtgc aaacaaaatc ccgccaggga tcgccatcag
     1501 cgatgccgct aacaggtagt caattggtac gcccattccg gcataaccaa tcatcatcga
     1561 accagcaatg gacgccatcc cgctacaaat tgcggtaaac aactcgttgc gattcatgcg
     1621 atcgataaac ggtttaacga tcgccgggat ctcattttgc ccgaggaaaa tagtggtaac
     1681 cgcaacaaaa gattcgattt tgctgatgtt gagagctttc tggaaaatgc tgccaagaat
     1741 gcgaatcagc agtcccatca cgccaatgta gtacagcagg ctgatgagcg cagtgacgaa
     1801 gataatcgcc gggagtacgc gaaaggcgaa gataaagccc gcgccatcaa acaggacatc
     1861 cattttcggc ccgaccaatg aaccaaaaat aaaggcgcta ccggcatcac tgtaagacat
     1921 cactttatga acgcctaatg ccgcctgttc tactgcccat tttcccggtg ggaagtagag
     1981 catgatgcca ccaatagcga tttgcagcag cagtgcggct ccaaccgtgc gcaaactgat
     2041 gcccttttta ttcactgaca acagaaatgc tattgccagt aacaccacca tccccacaac
     2101 acttctcatt atatccataa tgattttccc ttcatgccag taaacccggc gtcagcgcca
     2161 ggttttggta tgcttgatga gtacgggcga cggctttctg cccgtcagaa attaacccgc
     2221 gagacgctga tattctttgg caatttcgct cgccagaatg gcgttgttga acaccagctg
     2281 gatgttggat ttcaggctgt caccgccagt cagttcagca acacgagcca gcaggaacgg
     2341 tgtgctttct ttaccaataa cgccctgctc ttcagcttca gctaccgcct gatcgatcgc
     2401 cgcattgata gtgtgttccg gcatcgcaaa ctgttccggg atcggatttg ccaccaccag
     2461 gccaccgttc agaccgcttt gccatttcac cgccattgca cgagcgattt ccgtggcact
     2521 atcaaggcga atactaactt caaatgggct ggtacggcag aaaaacgcag gcagcgcttt
     2581 tgtctgatag ccaattaacg gcacaccgaa ggtttctaaa tattctgttg ttaatcctaa
     2641 atcgagaata gattttgcac cggcacaaac aacggtgaca ttagtatttg ccagttcttg
     2701 caaatcggca gaaatatcga aggtatgttc cgccccgcga tgcacaccac caattccccc
     2761 ggtggcaaat actttaattc cggcaagcgc cgcaataatc atcgttgaag caacggtagt
     2821 tgcgccattt tttccggcgg caacaacaaa aggtaaatcg cgacgactaa ctttggtcac
     2881 gttatgccct tcacgaccca gcaattcaat ttcttctttg cttaaaccca ctttcatcac
     2941 gccgccaata atggcgatag tggcaggtac agcgccctgt ttacgaatag tttcttcaac
     3001 ttcaattgcg gtctgggcat tttgtgggaa cggcatcccg tgagaaataa tggtcgattc
     3061 cagcgccaca accggttttt tgttttttaa agcgtcctgc acttccgggg aaatttgtaa
     3121 taattcaggg gaaattttta attcagacat tctgcgttct ccactaacga tataacgttg
     3181 gcaatcgata aatcggggtt attggtgtat tcacaggaga gcgccattga cgaacatccc
     3241 tgtgcgaaac gaacagattc ggcaaacggc attccgtcta cccaacacga agcaagtcca
     3301 gccatcatgg catcgcccgc tccggtaaca ttaatgacat tggttttgat cggcacagac
     3361 caaccacttt caccgctgat atcactgtaa taaacgccgt cgccgcccat gctcaatacc
     3421 agtcggttca ggccatgttg atggaaccag gcagcaactt ttgccacatc ttcacgccct
     3481 gacaaagcaa tcccactcag ggtttccgct tcaaggcggt ttggcttgag agtgtggatt
     3541 tgatttagac ggtcgcgcac tttgacacat ttccacgcgg aaacaggatc gacaaatacg
     3601 ggtacgttgg cggcattatc cagaatccat gccagtgcct cttcactgat attacagtcc
     3661 gcgacaatga cctttgccct ctgaataaat tcaccgtgct gtgcgagata ttcagctgta
     3721 atagcgttgc taatattcat gtcatttata gcaaccagca tttcaccggt attatcgagt
     3781 aatgataaat agctcgacgt attttctccc ggcacaatca ggcatttatc gacataaaca
     3841 ccagattgat tggtttgcgt tagcaacgat tgaccataaa aatcactgcc tactgcgctc
     3901 agtagccagg ctttattccc cagcaacgcc aggttttgtg caatattgcg ccctactcca
     3961 ccaggcgtaa attttatttt acctggattt gaatccgcat aatttaatga ttcatgtgaa
     4021 tatccggcga catcaatatt cgccgaacct ataattacga cataatcctt ttcgcgcata
     4081 gcacgtcctc tggtaaaaaa ttaccaaccc tgtcaatcca caagaatatt gatactgcaa
     4141 agacggttta aacatgtgtt catatttaaa catgtgctca tagtatgcat gttttacgta
     4201 aagtaaatta ccgccagaga ggcaaaatgg gcagatttga gcaggctcac aaaacgcgat
     4261 taaatacctg aattataaaa agggggaatt tgtgacggga tgcacagaac aggaacacta
     4321 aaaaagggca gaaattaatc tgcccttgag gaaataacct tagtgtttga tgaaagtttc
     4381 aggtttccac taaaagataa aaccatttaa attcataaaa ttacattcac aaaccggtca
     4441 ttctctttgc gcgcatcaaa tgttacaggg caggaaattt ctgccctgta acacaccttt
     4501 tattacgctg ctttcgctac tgcgtccact tccggacgtt tcaggaaggc ataggccaga
     4561 cccgccacca gcgtaccggc aataatggct accaggtaac ccaataccgg cgtaatcgcg
     4621 ccagggatca gcagaacaaa cagaccaccg tgcggtgcca tcagtttcgc accaatcgcc
     4681 attgagattg cgccagtcag cgccccaccc acgatacagc acggcagcac tcgcatcgga
     4741 tcacgggcag caaacggaat tgcaccttcc gaaatgaagc acagtcccaa taccagagcg
     4801 gctttgccac cttcctgctg cgctttgtcg aatttacgac gcgccaccat tgttgccagg
     4861 cccattgcca gcggtggcac catacctgcc gccataatcg ccgccatcgg gccataggtt
     4921 tgagtactca gcagacccac accgaacgcg tacgctgctt tgtttaccgg accgcccatg
     4981 tcagtacaca tcatgccacc gaggatcgcc cccagcagaa ccgcattcgc agtccccatg
     5041 gtctgcagcc agtgagtcaa cccttcgaga atgccagcaa ccggtttacc gatcaggtag
     5101 atcatcgcca gaccgaccac cagactggaa attagcggaa tgatcagaat cggtttcagc
     5161 gcctccatac tctgtggcag tttcaattgc gtactgatta acttcgcaat gtaaccagcc
     5221 aggaagcccg caataatacc gccaatgaag ccagaaccgg tgctgaccgc cagcataccg
     5281 ccaatcagac ccggcgtgag acccggacga tcggcaatgg aaaaggcaat ataacctgcc
     5341 agtaccggca ccatcaacgc aaaggctgaa ccaccaccaa tctgcatcag cgccgcagcc
     5401 aacgtacccg gctctttaaa cgcttcgata ccaaaagcaa aagaaagcgc gatacacaga
     5461 ccacctgcaa cgaccatcgg cagcatgtaa gagacgcccg tcagcaagtg acggtaagcg
     5521 cctgcactct ctttcttacc ttcagtggtc gctgtttgag ttttgcccgc cggttcatac
     5581 ggcgttgctt cagcaaccgc tttatccagt tcctgcgcgg ttttcttcag cgccagaccg
     5641 gtagaggtgc gatacatcgg tttaccagca aatttcgcca gatccacttc aatatctgcc
     5701 gccacaatca ccagatctgc tgcggctact tcttcagggg tgattgcatt acccgcgcca
     5761 accgaaccac gggtttcaac tttcacccac cagccacgtt ttttcgcttc ggtttcaatg
     5821 gcttcagccg ccataaaggt gtgtgctacg ccagtcgggc aagcagtcac cgcaactacg
     5881 cgtttcggac cgctggcggc aaccggtgct gtcgcagcga ccggcgcagt gtaaggtttc
     5941 gcatggcctt tggcttcact caggaacagc tcagggtgcg caactgcccg ggaaatatcg
     6001 cccagccaga catttttacc gttcagcgcg ctgtcgttcg ggatggaatc accgagaaca
     6061 atcgccattt cagcgtcgtt cggattgtcg atgatttcca gttttgcttt tcgcgccgcc
     6121 gcgcccagca gggtcttcgc catataggcg cgtgcctgac cgagattagc gtcaataatc
     6181 agcagcgttt tcattatgcc tctcctgctg tcagttaaaa ggttgtaagt cgacgcgcgc
     6241 catcattgcg gccaactgcg gacgatcggt aatacccaca ttgctttgac ttaccgccag
     6301 ggctgcaaca gctgtcgcca gacgcagtgt gtgttcactg gattcacgca tcagcaagcc
     6361 ataaatcagg ccaccaacca tagaatcccc tgcgccaacg gtgcttacga catcgactga
     6421 cggtggtttg gcgatccatt cgccggaggc attaacccaa agcgcgcctt cggcacccag
     6481 tgaaataacg acatgcgcga taccttgttc acgtagcgca tgtgcagctt caatcacatc
     6541 tttcatttca ggcagtttac ggcctgccca gatttccagc tcgcggcggt taggtttcac
     6601 cagccacggt gccgctttca aacctgctac taacgcttca cggctactat caaagataat
     6661 gcaaggacac tgactacgca ggcgagtcat ccagtcggtg aacgcttccg ggctgacgcc
     6721 tgacggtaag cttccgctga cacagaccat atcgaactga ccgagccagc tcagagaatc
     6781 agtcacaaag cgttcccagt cggcgggagt gacttcaaaa cccgagaagt tgaagtcggt
     6841 cacttcgccg tctttttccg tcagcttaac gttaattcgg gtgcgcccct gtacaacctg
     6901 gaaacggttg gcaatgccca gctcgctgaa cagttgctga aaaccatcct gattgtcttt
     6961 accgaggaag ccgccaacag tgacatcaat tcccaggtct tttaacactt tggccacgtt
     7021 gatgccttta cccgccgcat gcagaccggt ggttttcacc aggttcactt cgccgcgttc
     7081 aatttccggg cagaaaccaa caaggtcata agccggatta agggtgatag tagcaacacg
     7141 tctgctcatt atgcgccctc cccaagacca gcagcgatag cgtcgccaat ggctttcagc
     7201 gcctgttcag catctgcacc ctgggcggta aagcgtaggc gatgaccttt cttaacgcca
     7261 agtgccacaa ctttcatcag actacgtccg tttgccggtt tgccggtgcc atcaaggttt
     7321 gtcacggtaa tatcactgtt aaattgttta atggtattga ccagcatggt acctggacga
     7381 gcatgcaggc cgtgttcatt gcgcaccaca aactccgcgc ttaacacgtc gtcggtcggc
     7441 gcatcatcgc tggtcagcag cgccagcaac gttgccgcat ccgctttcag caagcggtca
     7501 gctttattgt cgagtaacaa atcagcgaga cgctttagaa ccgcaatggg ctgatcgtca
     7561 ttcatcgcca cactcaccag catggctgcc gtttcgccgt ccacatcaaa agcatttgcc
     7621 gcacggctta ccgcaatcgc gctacgcaga ttgccttcgg cgctatcgct cagccagata
     7681 ccctgtccga ggttcagcgg ttgttcattg atggctttgg tgacgaaagt ggcgtcaact
     7741 gcccccgcct ctttcagacg agcagcgttc agcgcctgaa gagtcagcag atcgctggcg
     7801 acgatatcca gcgtcagcat ttcgttgtcg agcttcagct gctcactctg cttttcgccc
     7861 atcagcaatg cgcgaagttc ttctgctgtt gttgctgact tcagttgttc agcaacggaa
     7921 tcatcgctca gtacgtgggt cagctggcgt agcaggccca aatgttcatc cgagctggca
     7981 gcaataccga tcgccacgta cgctacctgg ccgtcacccc aggtgacgcc ttccgggaac
     8041 tgaaatacct gaacgccggt tttcagcacc tgatcgcggg tgtcggtagt gccgtgtgga
     8101 atagcaatac cattgccgag gaacgtagag gtttgctgtt cgcgcgccag catgccattg
     8161 acgtagcctt cagctacatt accggcctgc accagcgccg cagcgacctg gcgaatcgcc
     8221 tcttctttgt ctccggcctt ttcgcccgga tggatgtcct gtacggataa ctggaacata
     8281 gttctcctct cttgctgaat tgaaacgatt cagcttctat gagaaaaaaa gcgccaacct
     8341 ggcttagggt taaagacaag atcgcgctga aacgtttcaa gaaagcataa tacttctgtt
     8401 tcagcacgca aggaaagccg taaagttatg agcaaaagtt tgatgtgctg cacaattttt
     8461 tgtcattttt cctaattgct gatgggaaaa tccgttatca gcaatttcat ttcagcagcg
     8521 tcagcagcca atcagcaaca gtgaaatggc attttgatta ttcgtggcgc ttttgatttc
     8581 gctgtctgtt tattttctga caagcagcgt aaactccgcg tcttcctctt ccagtgatcg
     8641 accagcatgc ataactcccc cgcagtctcc agcgcgaaat cgtttgacct gacctcgatg
     8701 gcgtttttaa tcgttgcctt tctcaccggt attgcgggcg ctctacaaac cccgacactc
     8761 agtatttttc ttactgatga agtacatgcc cgcccggcga tggtgggatt cttctttacc
     8821 ggcagcgctg tcattgggat tctggtaagt cagtttctcg ccgggcgctc tgataagcgc
     8881 ggcgatcgca aatcgctgat tgtcttttgc tgcctgttag gcgtgctggc ctgcactctt
     8941 tttgcctgga atcgcaacta ctttgttttg ctattcgttg gcgtctttct tagcagcttt
     9001 ggctcgactg ctaacccgca aatgtttgcc ctcgcccgtg aacatgctga caaaaccgga
     9061 cgtgaagcgg tgatgttcag ctctttttta cgcgctcagg tttcactggc atgggtcatt
     9121 ggcccgccgc tggcttatgc cttagcgatg ggtttcagct ttacggtaat gtatctgagc
     9181 gcagcggtag cgtttattgt ttgcggtgtg atggtgtggc tgtttttacc gtcgatgcaa
     9241 aaagaacttc cactggcgac cggcacggtt gaagcgccgc gccgtaaccg tcgcgatacg
     9301 ctgctgctgt ttgtcatttg tacattgatg tggggctcga acagcctgta catcatcaac
     9361 atgccgctat ttattatcaa cgaactgcat cttcccgaga aactggccgg tgtgatgatg
     9421 gggaccgccg ccgggctgga aatcccgacc atgttgattg ccggatattt cgccaaacgt
     9481 ttgggtaagc gtttcttaat gcgcgttgct gccgtgggtg gcgtctgttt ttacgcagga
     9541 atgctgatgg cgcattcacc tgtcattctg ttgggcttgc agctgcttaa tgctattttt
     9601 attggcattc tgggcggcat cgggatgctc tattttcagg atttgatgcc cggtcaggca
     9661 ggttcagcca ccacgctcta taccaacact tcgcgcgtgg gctggatcat cgcaggatca
     9721 gtggcgggca tcgtcgccga gatctggaat tatcacgctg tgttctggtt tgcgatggtg
     9781 atgattatcg ccactctgtt ttgcttactg cggattaaag atgtttaagg cgcggtcagc
     9841 atttccagat cgataagcca ggtcattgcc tgctgacgcg agttaccgca catttcagcg
     9901 ctggcttgta agcccgcaca aactttcggt cgcagtggcg aggtgaaaat tttacagcgt
     9961 tgctgttcat caagctgaat gcagggcgta ttggcgggct tgccatctgg cataccggga
    10021 atagggctgg aaattgaagg ggcagtgcaa cacgccccgc aacccggacg gcattccata
    10081 cgttgttctc ttaaggttaa ccaccggatt gatgagcgcg cacagtacca ccttttttgc
    10141 accagcaaaa gtgcgaatac cacttgcccg aaaggcccgt cgcgagtact ttgtcgcgat
    10201 atttttgaca ttttcgacta caggaatttt tcgatgccaa gagcgaacga aattaaaaaa
    10261 ggtatggtac tgaattacaa cggcaaactg ctgctggtta aggacattga tattcagtcg
    10321 cccactgccc gcggtgccgc tacgctgtac aaaatgcgtt tttctgatgt ccgtaccggg
    10381 ctgaaagtag aagagcgttt caaaggagat gatatcgttg acaccgtgac tctgacccgc
    10441 cgttacgttg acttctccta tgtcgatggt aacgaatatg tctttatgga taaagaagac
    10501 tataccccgt ataccttcac caaagatcag attgaagaag agttgctgtt tatgccagag
    10561 ggcggcatgc cggacatgca ggtgctgacc tgggacggtc aactgctggc gcttgagctg
    10621 ccgcagaccg ttgatctgga aatcgttgaa accgcacctg gcatcaaagg ggcatctgcc
    10681 agcgcccgta acaaaccggc gacattgagc actggtctgg tgattcaggt accagaatac
    10741 ttaagcccgg gcgaaaaaat tcgtatccat atcgaagaac gccgttatat ggggcgtgct
    10801 gactaacttc agccgcatgc agaaaaggga tagctcaggc tgtccctttt ttaatttatt
    10861 ataccaatct tctattttgc gcttcctgaa caccttacgc ccgcaatatt cactcgttga
    10921 ttgatcgccc tcacacttca tcgcattaac aatccagacc aatttcaatt gctgtcatat
    10981 aactttacac tgtcattgtt aattaatcgt tactaagacg tgactcctat gaatacaatc
    11041 gcctccgtta cgctcccgca tcatgtacac gctccacgct acgatcgcca gcagttgcaa
    11101 tcacgtatcg ttcattttgg ctttggtgcc tttcaccgcg cacatcaggc attactgacc
    11161 gatcgtgtgc tgaatgccca gggcggcgac tgggggatct gtgaaatcag cttgttcagc
    11221 ggcgatcaac tgatgagcca gctccgcgca cagaaccatt tatataccgt gctggagaaa
    11281 ggggcggacg gcaatcaggc gataattgtc ggtgccgttc acgaatgcct gaatgcaaaa
    11341 ctggattcct tagcggcaat tattgagaag ttttgcgagc cacaggtggc aattgtttcc
    11401 ctgacgatta ccgaaaaagg ctattgtatt gacccggcca ccggtgcact cgacaccagc
    11461 aatccgcgga ttattcacga tctacaaaac cctgaagaac ctcactccgc gccgggtatt
    11521 ctcgtcgaag cactgaaacg acgccgtgaa cgcggccttg caccgtttac cgtactctcc
    11581 tgcgacaata ttcccgacaa tggccatgtg gtgaaaaacg cggtgctggg gatggcggaa
    11641 aaacgttcac cagaactcgc cgggtggata aaagaacacg tcagttttcc gggaaccatg
    11701 gtcgaccgca ttgttccggc tgcaaccgac gaatcactag cggaaatcag ccagcatctg
    11761 ggggtgaatg atccctgcgc gattagctgc gaaccgttta tccagtgggt ggtggaagat
    11821 aacttcgtcg ctgggcgtcc tgcctgggaa gtcgcaggtg tacaaatggt gaatgatgtc
    11881 ctgccatggg aagagatgaa actgcgtatg cttaatggca gccactcttt tctcgcttat
    11941 ctgggttacc tctcaggatt cgcccatatc agtgattgca tgcaggatcg cgcatttcgc
    12001 catgccgcca gaacattaat gctggatgag caagcgccaa cactgcgaat taaagatgtc
    12061 gatttaacac aatatgcgga taagttaatt gcacgttttg ctaacccggc gctgaaacat
    12121 aagacctggc aaatcgcaat ggatggtagc cagaaattac cgcaacgtat gctggcaggt
    12181 attcgcatac atctggcgcg cgaaacggac tggtcgttgc tggcattagg cgttgcaggc
    12241 tggatgcgtt acgtcagcgg cgttgatgat gccggaaatg ccattgatgt tcgcgatccg
    12301 cttagcgata aaattcgcga acttgttgcg ggcagtagca gtgaacaacg cgtcaccgcc
    12361 ctgctttccc tgcgtgaagt tttcggtgat gatctgccag ataacccgca ttttgtgcag
    12421 gctatcgaac aagcctggca acaaatcgca caattcggcg cgcatcaggc gctattaaac
    12481 accctcaaaa tttaacgatt tctgcggtta aagcggatga agctcgcctt cgtccgctcc
    12541 ccccttctct tttctgcctt ttttagccag gattaacgct cagttaactt ttaagagtat
    12601 tgcggagccg ttgtgaccag gaccaacctc atcaccggtt ttctcggcag cgggaaaact
    12661 acgtcgattc ttcatctgtt agcccataaa gatcccaacg aaagatgggc ggtactggtt
    12721 aatgaatttg gggaagtcgg aattgatggt gctttgctcg ccgatagcgg cgcattgctg
    12781 aaagagatcc ccggcggctg catgtgctgc gttaatggtt tacccatgca ggtaggattg
    12841 aataccttac tgcgtcaggg aaaaccagac cgcttgttga tagagccgac cgggctgggc
    12901 catccgaaac aaatcctcga tctcttaacc gcaccagtct atgaaccgtg gatagatctg
    12961 cgcgccacgt tgtgcattct cgatccacgc ctgctgctgg acgaaaaaag cgccagcaat
    13021 gaaaacttcc gtgaccagct ggctgccgca gacattattg tcgccaataa atccgaccgt
    13081 gcgacgcccg aaagtgagca agcgctacag cgctggtggc agcaaaatgg tggcgatcgg
    13141 caattaattc acagcgagca tgggaaaatt gacggtcatc ttctggattt gccgcgtcgc
    13201 aatttagccg agttgcccgc cagcgccgca cattctcatc atcatagcgt gaaaaaaggg
    13261 ttagcagcgt taagcctgcc agagcatcaa cgctggcgtc gcagtctgaa cagcggacaa
    13321 gggcatcagg cttgtggctg gatattcgac gctgatacgg tattcgacac cattggcatt
    13381 ctggaatggg cgcgacttgc accggtggaa cgcgtcaaag gcgtgatgcg tattcccgaa
    13441 gggctggtgc gaatcaaccg tcagggcgat gacctgcaca ttgaaacgca aaacgttgcg
    13501 ccaccggaca gtcgtattga gctgatttcc agcagcgaag ctgactggaa tgccttgcag
    13561 agcgcgctgt tgaagcttcg tttagcgact accgcgtaag gttgcctgcg tttttcagta
    13621 agataattag agaaaatatg attaaaaatt tgccgcaaat agtgttgttg aatattgtcg
    13681 gcctcgcgct gtttctttcc tggtatattc ccgttaatca tggattctgg ttgccgattg
    13741 atgcggatat tttttatttc tttaatcaga aactggtcga aagtaaggcc tttttgtggc
    13801 tggttgcatt gaccaacaat cgcgctttcg acggttgttc tctgctggcg atgggaatgt
    13861 tgatgctgag tttctggctg aaagaaaacg cccctggcag acgacgtatc gtgattattg
    13921 gtctggtcat gctattaact gcagtggtat taaaccagct gggtcaggca ttaattcctg
    13981 taaaacgggc cagcccaaca ttgactttta ccaatattaa ccgcgtcagc gaactgctct
    14041 ctgtccccac gaaagatgcc tcacgagata gctttcccgg tgatcacggt atgatgctgc
    14101 ttattttttc ggcattcatg tggcgttatt tcggcaaagt tgcaggcctt atcgccctta
    14161 ttatttttgt ggtttttgca tttcccagag taatgattgg cgcacactgg tttactgaca
    14221 tcattgtagg ttcgatgacc gtgatattga tcggtttgcc ctgggtgttg ctgacgccat
    14281 taagtgatcg attaatcacc ttttttgaca aatcactacc gggaaaaaac aaacatttcc
    14341 aaaacaaata actcacagta attaacatca tcagggttat ttttatagtg aggataatcc
    14401 tgatgatgcg caccgtgctt tcatctatcg aacgcaaaaa tcattctcta agtaaatgaa
    14461 tggattgcat gcgcttcact caatcgtact ttaattgacc aatcccgctt attaactttc
    14521 tgtatcactt tttcttataa aaaatcatgt aaaaccgctc gccaagaccg caccaatcgg
    14581 gtaatctcga actcgttttg cctcggcggt agattatcct cacagcatat aattttgtgc
    14641 gttagtccac agatttggcc ttaaggaatt gtttcaacat gcccaggtaa ttagtctcgt
    14701 gtcgcttggc atttttttat aacgatattt gtcgttaagg acttcaaggg aaaacaaaca
    14761 acatggtcaa atctcaaccg attttgagat atatcttgcg cgggattccc gcgattgcag
    14821 tagcggttct gctttctgca tgtagtgcaa ataacaccgc aaagaatatg catcctgaga
    14881 cacgtgcagt gggtagtgaa acatcatcac tgcaagcttc tcaggatgaa tttgaaaacc
    14941 tggttcgtaa tgtcgacgta aaatcgcgaa ttatggatca gtatgctgac tggaaaggcg
    15001 tacgttatcg cctgggcggc agcactaaaa aaggtatcga ttgttctggt ttcgtacagc
    15061 gtacattccg tgagcaattt ggcttagaac ttccgcgttc gacttacgaa cagcaggaaa
    15121 tgggtaaatc tgtttcccgc agtaatttgc gtacgggtga tttagttctg ttccgtgccg
    15181 gttcaacggg acgccatgtc ggtatttata tcggcaacaa tcagtttgtc catgcttcca
    15241 ccagcagtgg tgttattatt tccagcatga atgaaccgta ctggaagaag cgttacaacg
    15301 aagcacgccg ggttctcagc cgcagctaat aaaccgtttg gatgcaatcc cttggctatc
    15361 ctgacgagtt aactgaaagc actgcttagg cagtgctttt ttgttttcat tcatcagaga
    15421 aaatgatgtt tccgcgtctt gatccaggct atagtccggt cattgttatc ttttaaatgt
    15481 tgtcgtaatt tcaggaaatt aacggaatca tgttcatacg cgctcccaat tctggacgta
    15541 agctcctgct tacctgcatt gttgcaggcg tgatgattgc gatactggtg agctgccttc
    15601 agtttttagt ggcctggcat aagcacgaag tcaaatacga cacactgatt accgacgtac
    15661 aaaaatatct cgatacctat tttgccgacc tgaaatccac tactgaccgg ctccagccgc
    15721 tgaccttaga tacctgccag caggctaacc ccgaactgac tgcccgcgcg gcatttagca
    15781 tgaatgtccg tacgtttgtg ctggtaaaag ataaaaaaac attctgttca tctgcgactg
    15841 gtgagatgga cattccactc aatgaattga ttccggcgct cgacattaat aaaaacgtcg
    15901 atatggcgat cttacccggc acgccgatgg tgccgaacaa acccgcaatc gtcatctggt
    15961 atcgcaaccc tttgctgaaa aatagcggcg tgtttgccgc tctgaatctc aatctgacgc
    16021 cttctctctt ttatagttca cggcaggaag attacgatgg cgtcgccctc attattggca
    16081 atactgcgct atctaccttt tcttcacgtt tgatgaacgt taatgaatta accgacatgc
    16141 cggtccgtga aactaaaatt gcgggcattc ctctgaccgt tcggctttat gcagatgact
    16201 ggacatggaa cgatgtgtgg tacgcatttt tactgggcgg catgagtgga actgtcgttg
    16261 gcctgctctg ctattacctg atgagcgtac gtatgcgccc cggcagagaa atcatgaccg
    16321 ccatcaagcg cgaacaattt tacgtggcgt atcaaccggt ggtggataca caatctttgc
    16381 gagtaacggg cctggaagta ctgctacgct ggcggcatcc tgtcgcggga gaaatacccc
    16441 cggatgcctt cattaacttt gccgaatcgc aaaagatgat tgtgccactg actcagcacc
    16501 tgtttgagtt gattgcccgc gatgccgcag aattagaaaa agtactgccg gtaggcgtca
    16561 aatttggcat taacattgcg ccggaccatc tgcacagcga aagctttaaa gcagatatcc
    16621 agaaactgct cacttccctg cccgcacacc atttccagat tgtgctggaa attaccgagc
    16681 gcgatatgct gaaagagcaa gaagccacgc aactctttgc ctggctacac tcggtcggcg
    16741 tagaaattgc tattgatgac ttcggtaccg ggcacagcgc gcttatctac cttgagcgtt
    16801 ttacgctcga ttatctgaaa attgaccgtg gatttatcaa cgccatcggt acggaaacga
    16861 tcacttcacc cgtacttgac gcggtgctga cgctggcgaa acgtctcaat atgctgacgg
    16921 ttgctgaagg ggtcgaaacg ccggaacagg cgcgatggct aagcgaacgc ggcgttaatt
    16981 tcatgcaagg ctactggatt agtcgcccgt taccgctgga cgattttgtc cgctggctga
    17041 agaaaccgta tacgccgcag tggtaaggtg tgcttacgtc ccttattatt catagtgaaa
    17101 gcatgccgga ttgcggctaa tgatgagtaa aaggaaatcc gttgcagatg attgtgcgca
    17161 tactgctgct gtttatcgct ctgttcacct ttggtgcgca ggcgcaggct atcaaggaaa
    17221 gctatgcctt tgccgtgctg ggcgaacccc ggtacgcgtt taatttcaac cattttgatt
    17281 atgtgaaccc cgccgcgcca aaaggggggc agataacgtt gtcagccctc ggcaccttcg
    17341 ataatttcaa ccgctatgca ctgcgcggca acccgggcgc acgcaccgag cagctgtacg
    17401 acacgctatt tacaacttcc gatgacgaac caggcagtta ttacccgctg attgctgaaa
    17461 gtgcacgcta tgctgacgat tattcctggg tggaggtcgc tattaatccg ctcgcccgtt
    17521 ttcatgatgg ttcgcccatt actgcccgcg atgtagagtt tacttttcaa aaatttatga
    17581 ccgaaggcgt gccgcaattt cgtctggtct acaaaggcac caccgtcaaa gccattgcgc
    17641 cgttaaccgt gcgcattgag ttagctaaac ccggcaaaga agatatgctg agtctgtttt
    17701 cgctgccggt gtttccagaa aagtactgga agaatcacaa acttagcgac ccgctcgcca
    17761 cgcctccgct tgccagtggt ccgtaccgaa ttacgtcctg gaaaatgggg caaaatattg
    17821 tctattcccg cgtgaaagat tactgggcag caaacttacc ggtaaaccgt ggacgctgga
    17881 atttcgacac cattcgctac gattattacc tcgatgataa tgtcgccttt gaagcgttta
    17941 aagccggtgc ctttgatttg cgtatggaaa acgacgccaa aaactgggcc acgcgctata
    18001 ccggtaaaaa tttcgataag aaatacatca tcaaagatga gcaaaagaac gaatcagccc
    18061 aggatacgcg ctggctggcg tttaatatcc aacgtccggt attcagcgat cgccgggtgc
    18121 gggaagcgat cactctcgcc tttgactttg aatggatgaa caaagcgttg ttttacaatg
    18181 cctggagtcg cacgaacagt tattttcaga ataccgaata cgcggccaga aattaccccg
    18241 acgccgcgga gctggtgctt ctggcaccaa tgaaaaaaga tctaccgcca gaagtcttca
    18301 cgcaaatcta ccagccgccg gtatccaaag gcgatggcta cgatcgtgac aacctgttaa
    18361 aagccgacaa acttctcaac gaagcgggct gggtgctgaa gggtcagcaa cgcgtaaatg
    18421 ccacaacggg tcagccgctc agctttgagt tattgctacc ttcgagcagc aatagtcagt
    18481 gggtattgcc gttccagcac agcctgcaac ggctgggtat caacatggac attcgcaaag
    18541 tggataactc gcaaatcacc aaccgcatgc gcagtcgcga ctatgacatg atgccgcgcg
    18601 tatggcgggc gatgccgtgg cccagttccg atttacagat ttcctggtca tcggaatata
    18661 tcaattccac ttataatgcc cccggcgtgc aaagcccggt tatcgactcg ctgatcaacc
    18721 aaattattgc cgcgcaggga aataaagaaa aattactgcc gttggggcga gcactggatc
    18781 gcgtattaac gtggaattat tacatgctgc caatgtggta catggcggaa gaccgtctcg
    18841 cctggtggga taaattctcc caacccgctg tacgccctgt ttacagcctg ggtatcgata
    18901 cctggtggta tgacgttaat aaagcggcca aactgccgtc agccagacaa cagggagagt
    18961 agatgggcgc ttatctgatt cgccgtctgt tgctggtgat cccaacgtta tgggcgatta
    19021 tcactatcaa ctttttcatc gtgcaaattg cgcctggcgg tccggtcgac caggccatcg
    19081 ccgccattga gtttggtaat gccggagtat tacccggcgc aggcggtgaa ggtgttcgcg
    19141 ccagccatgc gcaaacgggc gtcggcaata tcagcgacag taattaccgt ggcggacgcg
    19201 gattagatcc agaagtgatc gccgagatca ctcatcgcta cggtttcgat aagccgatcc
    19261 acgaacgtta cttcaaaatg ctctgggact acatccgctt tgattttggc gacagccttt
    19321 ttcgcagcgc ctcggtactg acgctgatta aagatagtct gccggtttcc atcaccctcg
    19381 gattatggag cacgctgatt atctatctgg tgtcgattcc gttaggcatt cgcaaagctg
    19441 tttataatgg tagtcgcttt gacgtctgga gtagtgcatt tatcatcatc ggctacgcca
    19501 ttccggcctt tttgtttgcc atcctgctga ttgtcttctt cgcaggcggc agctatttcg
    19561 acctgttccc tctacgcggc ctggtttccg ctaactttga ttcgctgccg tggtatcaga
    19621 aaatcaccga ttatctgtgg catatcacgc tgccggtgct ggcgacagtg attggcggct
    19681 ttgcggcgct gaccatgctg acaaaaaact cattccttga tgaagtgcgc aagcaatacg
    19741 tggtgaccgc ccgtgcgaaa ggggtaagtg aaaaaaatat tctctggaaa catgtgttcc
    19801 gcaacgccat gctgctggtg attgccggtt ttccggcgac gtttatcagc atgtttttta
    19861 ccggctcgct gctgattgag gtgatgtttt cactcaatgg tcttggctta ctgggctacg
    19921 aagcgaccgt ctcgcgcgat tatcctgtaa tgtttggtac cttgtatatt ttcaccctga
    19981 ttggcctgct gctgaatatt gtcagtgata tcagctatac gctggtcgat ccgcgtatag
    20041 attttgaggg acgttaatgt cgcgactcag ccccgtcaat caggcccgtt gggcgcgttt
    20101 tcgtcataac cgtcgcggct actggtcgtt atggattttc ctcgttttgt ttggtttgag
    20161 tttgtgttct gaactgatcg ccaacgataa accgttgctg gtacgttatg acggcagttg
    20221 gtatttcccg ttgttgaaaa actacagcga aagcgatttt ggcggcccgc tggcaagcca
    20281 ggctgattat caggacccgt ggctgaaaca gcggttggaa gataaaggct gggtactatg
    20341 ggcaccgatt cgctttggcg ctaccagtat caac
//
LOCUS       DACBVI010000077        20142 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.077, whole genome shotgun sequence.
ACCESSION   DACBVI010000077 DACBVI010000000
VERSION     DACBVI010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.077"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(75..659)
                     /locus_tag="IU325_13595"
     CDS             complement(75..659)
                     /locus_tag="IU325_13595"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP5810045.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            937..1215
                     /gene="ychH"
                     /locus_tag="IU325_13600"
     CDS             937..1215
                     /gene="ychH"
                     /locus_tag="IU325_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HAP5810046.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(1270..2949)
                     /gene="dauA"
                     /locus_tag="IU325_13605"
     CDS             complement(1270..2949)
                     /gene="dauA"
                     /locus_tag="IU325_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HAP5810047.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLMSGVFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNLEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(3074..4021)
                     /gene="prs"
                     /locus_tag="IU325_13610"
     CDS             complement(3074..4021)
                     /gene="prs"
                     /locus_tag="IU325_13610"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP5810048.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(4172..5023)
                     /gene="ispE"
                     /locus_tag="IU325_13615"
     CDS             complement(4172..5023)
                     /gene="ispE"
                     /locus_tag="IU325_13615"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP5810049.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGADISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(5023..5646)
                     /gene="lolB"
                     /locus_tag="IU325_13620"
     CDS             complement(5023..5646)
                     /gene="lolB"
                     /locus_tag="IU325_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HAP5810050.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            5860..7116
                     /gene="hemA"
                     /locus_tag="IU325_13625"
     CDS             5860..7116
                     /gene="hemA"
                     /locus_tag="IU325_13625"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HAP5810051.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            7158..8240
                     /gene="prfA"
                     /locus_tag="IU325_13630"
     CDS             7158..8240
                     /gene="prfA"
                     /locus_tag="IU325_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP5810052.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDVVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            8240..9073
                     /gene="prmC"
                     /locus_tag="IU325_13635"
     CDS             8240..9073
                     /gene="prmC"
                     /locus_tag="IU325_13635"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP5810053.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEITAVDRMPDAVSLAQRNAQNLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            9070..9462
                     /locus_tag="IU325_13640"
     CDS             9070..9462
                     /locus_tag="IU325_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HAP5810054.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIAFDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            9466..10275
                     /locus_tag="IU325_13645"
     CDS             9466..10275
                     /locus_tag="IU325_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HAP5810055.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            10311..11165
                     /gene="kdsA"
                     /locus_tag="IU325_13650"
     CDS             10311..11165
                     /gene="kdsA"
                     /locus_tag="IU325_13650"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HAP5810056.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            11360..11818
                     /gene="tnpA"
                     /locus_tag="IU325_13655"
     CDS             11360..11818
                     /gene="tnpA"
                     /locus_tag="IU325_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP5810057.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKRRAIGSILRKL
                     CEWKSVRILEAECCADHIHMLVEIPPKMSVSGFMGYLKGKSSLMLYEQFGDLKFKYRN
                     REFWCRGYYVDTVGKNTAKIQDYIKHQLEEDKMGEQLSIPYPGSPFTGRK"
     gene            complement(12024..12131)
                     /locus_tag="IU325_13660"
     CDS             complement(12024..12131)
                     /locus_tag="IU325_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic polypeptide LdrA/LdrC"
                     /protein_id="HAP5810058.1"
                     /translation="MTLTQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            complement(12559..12666)
                     /locus_tag="IU325_13665"
     CDS             complement(12559..12666)
                     /locus_tag="IU325_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic polypeptide LdrA/LdrC"
                     /protein_id="HAP5810059.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            complement(13071..14171)
                     /gene="chaA"
                     /locus_tag="IU325_13670"
     CDS             complement(13071..14171)
                     /gene="chaA"
                     /locus_tag="IU325_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HAP5810060.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIGINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIVHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            14441..14671
                     /gene="chaB"
                     /locus_tag="IU325_13675"
     CDS             14441..14671
                     /gene="chaB"
                     /locus_tag="IU325_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HAP5810061.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            14829..15524
                     /locus_tag="IU325_13680"
     CDS             14829..15524
                     /locus_tag="IU325_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAP5810062.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(15568..15921)
                     /gene="ychN"
                     /locus_tag="IU325_13685"
     CDS             complement(15568..15921)
                     /gene="ychN"
                     /locus_tag="IU325_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HAP5810063.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            16107..17501
                     /gene="ychO"
                     /locus_tag="IU325_13690"
     CDS             16107..17501
                     /gene="ychO"
                     /locus_tag="IU325_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HAP5810064.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGSWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(17502..18152)
                     /gene="narL"
                     /locus_tag="IU325_13695"
     CDS             complement(17502..18152)
                     /gene="narL"
                     /locus_tag="IU325_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HAP5810065.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDISVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18145..19941)
                     /gene="narX"
                     /locus_tag="IU325_13700"
     CDS             complement(18145..19941)
                     /gene="narX"
                     /locus_tag="IU325_13700"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HAP5810066.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAEGDGQLAQLQGL
                     QDYWRNELIPTLMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVLVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
BASE COUNT         5080 a         4991 c         5254 g         4817 t
ORIGIN      
        1 ctaacagatt gcagaaatgg aaataacttt gcctattata cacggcactc ggcaaaaatg
       61 ccgcagacaa cgacttattg cgctttaaag gcgtgcaatc ggttcgttgc tttggtcaag
      121 ccatctgtaa accacatttc agtacaacgc gccgcttcgt cgatggcttc atcaattaac
      181 ttctgttcac taacaggcgg tttgcctaat acaaaaccaa caactttatt tttatcgccc
      241 ggatgaccga ttccgatgcg taaacggtga aagttagggt tattacccaa tttactgatg
      301 atgtctttca gtccattgtg accaccatgg ccaccgccca acttaaattt ggcgacgcca
      361 ggaggcagat ccagttcgtc gtgggccacc agaatttcgt ccggattaat gcggaaaaaa
      421 ctggccatcg ccgcaacggc tttgccgctg agattcataa atgtagtcgg gactaacagg
      481 cggacatctt cgcctccaag ggtgactcgc gaagtataac caaagaattt agcctcttcg
      541 cgcagcggag cgcgcaatcg ctctgccagt aagtcaacga accaggcacc agcattatgt
      601 cgcgttgcgg cgtattcagc gccggggttc gccaggccga caatcaattt aatcgtcacg
      661 tttttttgtc ctgagtgtgt acataactgg cgcgtagttt actggttgcg gccccgcttg
      721 acaaaaaact gcgtatcaaa tgcagataac gcaataattg cctgagtgga ctattagaaa
      781 gtcaaggtgt tcaggcgttt atttgtaaag ttttgttgaa ataagggttg taattgtgat
      841 cacgcccgca cataacccac agggtgttgt ctatacttta cacataagga agaggggtat
      901 tccctgttac aacccagaaa gttccggagg tgacatatga aacgcaaaaa cgcttcgtta
      961 ctcggtaacg tgctcatggg gttgggtctg gtggtaatgg tggtcggcgt ggggtattca
     1021 atcctcaacc agttaccaca gtttaatatg ccccagtatt tcgcacatgg tgcagtgcta
     1081 agtattttcg tcggtgccat tctctggctg gcgggtgccc gtgttggcgg gcatgaacag
     1141 gtgtgcgacc gttactggtg ggttcgccac tatgacaaac gttgccgccg tagcgataat
     1201 cgccgtcata gctaacaaat aatgcagttt gctgaccagt cagtttgcgc tgactggtca
     1261 atctcgtact tataaatccg ccatcgccgc gcgacgattc gggaagaatg cgaggcgtcc
     1321 cgggatcggt tgaatgcctg cgcgagccat agtgcgcagt ggctggaatt ccaggttgca
     1381 cacgcgcagt tcacatcctt cgggcagacg ctttacaaaa cgctggaacg catcaagacc
     1441 accagcatca agtaccggaa cggcatccca cttcagaatc acaatccgtt tgccttcaag
     1501 acgtgactcc aggtccgtga acaagccttc agcggcagca aaaaacagcg gaccaataac
     1561 gcgcagaacc agaacatcgt ctggaacgtc tacgactacc ggtgccaggc gagtcatacg
     1621 tgcgatacga cgcataaaca gtagcgatgc cagcacgatc cccacgctga tggcaataac
     1681 catatcaaac agcacagtca gcgacatgca cagcagcatg acgatgatgt catctttcgg
     1741 cgcatgacgc agcaagtcga ccactttgtg cgcttcactc atgttccacg ccaccatcaa
     1801 cagcagggca gccatggcgg aaagcggcag ccaggagagc agcggtgcca gtaccagcag
     1861 ggcaagaata accagaatag agtggatcac cgccgagata ggggaagttg ccccggcacg
     1921 gacgttagcg gcagaacgcg cgatggcagc tgtagcggta ataccaccaa agaacggggc
     1981 gatgatattc cccagtccct gtccaaccag ttcgctgttc gctttgtgtt tcgttccggt
     2041 cataccatcc agtaccacgg cgcagagcag agattcgatt gcgccgagca ttgccattga
     2101 gaatgccgca ggcagcagtg tgcgaataga atcccaggtg agggtaaatt ctgaattggg
     2161 caggtcccac ggtagtacca gttgcggcag cagttgcgga ataccgttac cctgagaacc
     2221 atctgccaga acgtagtgga attgcgatcc gatggtagca acatgcccgc cgagcaggtt
     2281 aacaatcccc atcaccgcgc aaccagccag caaggccgga aggtgaccag gcaaacgaat
     2341 gcccagacgc ggccagaaaa ccagaatgcc aagcgtcaca atgccaatgg cagcatcacc
     2401 cacattaatg gtcggcaacg ccataaataa tgcgccgact ttttgtagat aatgctccgg
     2461 gacatgggcc atttgcagac cgagaaaatc tttaatctgc atggtaccga tggtgatccc
     2521 gatacccgag gtgaaaccta aggtcacgga aaccggaata tattcaatca agcgtccaaa
     2581 acgtgccagc cccatcagga tcaaaaagac acctgacatg agtgtcgcga ccagcagtcc
     2641 tgccagtcca aactgttgcg aaacgggata gagaattacc acaaatgccg cagtcggacc
     2701 ggaaacgcta aagcgtgacc cacccgtcag agcaatgaca atccccgcaa cagctgcggt
     2761 atataaaccg tactggggtg ccacaccact accaatagcc aacgccattg ccagcgggat
     2821 agcaataatc ccgacggtta tcccggcaat caggtcacgg gtaaaccgtg cggcagtata
     2881 tttttctttc cagcaagcgt cgatcagagc gcggaaaggc atcacatgtg aggaaaatat
     2941 tttgttcaca ataatgtttc atccgtgagc gcatcatctg tcaactaaat ggcaggtgaa
     3001 ggaggcatag gtcatacaaa tggatattac agacaaaaaa acccgccgca gcgggtcttt
     3061 gagccgggtt cgattagtgt tcgaacatgg cagagatcga ttcttcgttg ctgatacgac
     3121 gaatcgcttc ggccagcata cctgacaggg tcagagtacg cacgttcggc agtgatttga
     3181 tttcatcgct cagcggaatg gtatcgcaga caacgacttc atcaattaca gagttacgca
     3241 ggttgttcgc cgcgttgcca gagaagatcg ggtgagtcgc gtacgcaaat acacgtttag
     3301 caccacgttc tttcagagct tcagcagctt tacacagcgt accgccagta tcgatcatat
     3361 catcgaccag tacgcagtca cgacctgcaa cgtcaccgat gatatgcatc acctgggaaa
     3421 cgttcgcacg cggacgacgt ttgtcgatga ttgccatatc ggtatcgttc agcagcttag
     3481 cgatagcgcg ggcacgcaca acgccgccga tgtccggaga aaccacaata gggttatcca
     3541 gattcagctg cagcatgtct tccagcagga tcgggctacc aaatacgtta tcaaccggaa
     3601 cgtcgaagaa accctgaatc tgttcagcgt gcagatccac tgtcagcaca cggtcaacac
     3661 cgacgctgga gaggaagtct gcaaccactt tcgcagtgat tggtacacga gcggaacgga
     3721 cgcgacggtc ctggcgcgca tagccaaagt aggggataac agcggtgata cgacctgcgg
     3781 aagcacgacg cagggcatca accataacga ctaattccat caggttgtcg ttagtagggg
     3841 cacaagtgga ctggatgatg aaaatatcac caccgcgtac attttcgtta atttgtacgc
     3901 tgacttcgcc gtcgctaaag cgacctacag cggcgtcgcc gagtgaagtg tacaggcggt
     3961 tggcaatacg ttgtgctagt tccggggtgg cgttaccagc aaaaagcttc atatcaggca
     4021 cgagaagaac ctcaggcatg cgtccattgg tggaaagaat ctgccgaaaa ctgtacgggc
     4081 caggcatgat tctttccagg cggtgtatta aagagcgcga tgcaacgtct ggaacaaggt
     4141 gacgttgtca ccgaaactca gcttgcccgg cttaaagcat ggctctgtgc aatggggaaa
     4201 gattaacgcc tttcgccaca aagccattga gccattccgg ggcttgctct agcacctggc
     4261 gggcttcaga ctctgtatca aattcagcaa agacacaggc ccctgtccca gtcaggcgcg
     4321 acggggcgta ttctaacagc caggaaagca ccgcatcaac ctcgcgaaaa cgttttcttg
     4381 cgataacctc gcaatcattg ctgaattcac attttagcaa cgtttctatt gacctttttg
     4441 gcgtattgcg cgggagttca ggatctttaa aaatcactgg tgtcggaata cttacaccag
     4501 ggtgcgccac cagataccac ttctctggcg gatccaccgg cgttagtatt tcaccaacgc
     4561 cttcggcaaa cgcggcatgc ccccgaacaa agacaggaac atctgcgccc agcgtcagcc
     4621 ccatttccgc cagctcatcc atgcttagcc cgcattgcca gagatgattt aatgccacca
     4681 ggaccgtcgc ggcattggat gaaccaccgc cgagaccgcc gcccatcggc aaacgcttgt
     4741 caatgctgat atccgcaccg cttcccgtcg gaagacgccc gctgtctgcc gcagttttca
     4801 tcaacaatcg tgctgcgcga acgatcaggt tatcttcatg ttccacgcct tcaacgggcg
     4861 ttaacagacg aatatcccca tcgtcacgaa gctcaatgct gatggtgtcg ccgtaatcaa
     4921 gaaactgaaa cagcgtttgc agcgtgtggt aaccatccgc acgctgaccg gtaatgtata
     4981 aaaacagatt aagttttgcc ggagagggcc actgtgtccg cattatttca ctatccagtt
     5041 atccattttt aacttgatgc gttgaccacc gtcggtgagt tccatattgg ctggcatcgc
     5101 aggttgagtt tttgtgtcat aaccaccgta aacaactttc cagtttttgc cattctggct
     5161 gtaggtgatt tcgctcaggc ggtactggtc gtccagtttg tagtcggttg catcacccgg
     5221 taaacctaaa atccactggc gcaagctgtt gagcggaatt ggcattccgg tcaatttgcc
     5281 aatcatctct tcggcgtcat cggcggtata acgctggcct ttattgtcga ctaactgcac
     5341 gttacccggt tgagcattca gctccagttc cgtgctgcct aatgggttag tcagcagcag
     5401 acggtagcga tcctggccag tttgctgcca gaagaaacgg gcgtacactt tttgttggtc
     5461 agaaatataa gcgaacgcgc cgcgagtttg atactgatta agattgcgca cgtcttgctg
     5521 atgctgacgc cattgtggtg aatccgggct tttgccagga cctttgggtg tggtaacgga
     5581 acaggcagtg agcacaagag cagccagcgg tagcaggcgg ataagacgaa aatcgggcag
     5641 gggcatagtg atgacaagtc cttgagatac gttgcagtta taacccttaa tgctagcgtt
     5701 accgtccgct atcgtctatg ttcaagttgt cttaattgcc agaatctaac ggctttcggc
     5761 aattactcca aaagggggcg ctctctttta ttgatcttac gcatcctgta tgatgcaagc
     5821 agactaaccc tatcaacgtt ggtattattt cccgcagaca tgaccctttt agcactcggt
     5881 atcaaccata aaacggcacc tgtatcgctg cgagaacgtg tatcgttttc gccggataag
     5941 ctcgatcagg cgcttgacag cctgcttgcg cagccgatgg tgcagggcgg cgtggtgctg
     6001 tcgacgtgca atcgcacgga actttatctt agcgttgaag agcaggataa cctgcaagag
     6061 gcgttaatcc gctggctatg cgattatcat aatcttaatg aagaagatct gcgtaaaagc
     6121 ctctactggc atcaggataa cgacgcggtt agccatttaa tgcgtgttgc cagcggcctg
     6181 gattcactgg ttctggggga gccgcagatc ctcggtcagg taaaaaaagc ctttgctgat
     6241 tcgcaaaaag gtcatatgaa ggccagcgaa ctggaacgca tgttccagaa atctttctct
     6301 gtagcgaaac gcgttcgcac tgaaacagat atcggtgcca gtgctgtgtc tgtcgctttt
     6361 gcggcttgta cgctggcgcg gcagatcttt gaatcgctct ctacggtgac ggtactgctg
     6421 gtgggtgcgg gcgaaaccat cgagctggtg gcgcgtcatc tgcgcgaaca taaagtacag
     6481 aagatgatta tcgccaaccg cactcgcgaa cgtgcccaaa ttctggcaga tgaagtcggc
     6541 gcggaagtta ttgccctgag tgatatcgac gaacgtctgc gcgaagccga tatcatcatc
     6601 agttccaccg ccagcccgtt accgattatc gggaaaggca tggtggagcg cgcattaaaa
     6661 agccgtcgca accaaccgat gctgctggtg gatattgccg ttccgcgcga tgttgagccg
     6721 gaagttggca aactggcgaa tgcttatctt tatagcgtgg acgatctgca aagcatcatt
     6781 tcgcacaacc tggcgcagcg taaagccgca gcggttgagg cggaaactat tgtcgctcag
     6841 gaaaccagcg aatttatggc gtggctgcgt gcacaaagcg ccagcgaaac cattcgcgag
     6901 tatcgcagcc aggcagagca agttcgcgat gagttaaccg ccaaagcgtt agcggccctt
     6961 gagcagggcg gcgacgcaca agccattatg caggatctgg catggaaact gactaaccgc
     7021 ttgatccatg cgccaacgaa atcacttcaa caggccgccc gtgacgggga taacgaacgc
     7081 ctgaatattc tgcgcgacag cctcgggctg gagtagcagt acatcatttt ctttttttac
     7141 agggtgcatt tacgcctatg aagccttcta tcgttgccaa actggaagcc ctgcatgaac
     7201 gccatgaaga agttcaggcg ttgctgggtg acgcgcaaac tatcgccgac caggaacgtt
     7261 ttcgcgcatt atcacgcgaa tatgcgcagt taagtgatgt ttcgcgctgt tttaccgact
     7321 ggcaacaggt tcaggaagat atcgaaaccg cacagatgat gctcgacgat cctgaaatgc
     7381 gtgagatggc gcaggatgaa ctgcgcgaag ctaaagaaaa aagcgagcaa ctggaacagc
     7441 aattacaggt tctgttacta ccaaaagatc ctgatgacga acgtaacgcc ttcctcgaag
     7501 tccgtgccgg aaccggcggc gacgaagcgg cgctcttcgc tggcgatctg ttccgtatgt
     7561 acagccgtta cgccgaagcc cgccgctggc gtgtagaaat catgagcgcc agcgagggtg
     7621 aacatggtgg ttataaagag atcatcgcca aaattagcgg tgatgttgta tatggtcgtc
     7681 tgaaatttga atccggcggt catcgcgtgc agcgtgttcc tgctacggaa tcgcagggtc
     7741 gtattcatac ttctgcttgt accgttgcgg taatgccaga actgcctgac gcagaactgc
     7801 cggacatcaa cccagcagat ttgcgcattg atactttccg ttcgtcaggg gcaggtggtc
     7861 agcacgttaa caccaccgat tcggcaattc gtattaccca cttgccgacc gggattgttg
     7921 ttgaatgtca ggacgaacgt tcacagcata aaaacaaagc taaagcactt tctgtgcttg
     7981 gtgctcgcat ccacgctgca gaaatggcaa aacggcaaca ggccgaagcg tctacccgtc
     8041 gtaacctgct ggggagtggc gatcgcagcg accgtaaccg tacttacaat ttcccgcagg
     8101 ggcgcgttac cgatcaccgc atcaacctga cgctctaccg tctggatgaa gtgatggaag
     8161 gtaagctgga tatgctgatt gaaccgatta tccaggaaca tcaggccgac caactggcgg
     8221 cgttgtccga gcaggaataa tggaatatca atactggtta cgtgaagcaa taagccaact
     8281 tcaggcgagc gaaagcccgc ggcgtgatgc tgaaatcctg ctggagcatg ttaccggcag
     8341 agggcgtact tttattctcg cctttggtga aacgcagctg actgacgaac aatgtcagca
     8401 acttgatgcg ctactgacac gtcgtcgcga tggtgaaccc attgctcatt taaccggggt
     8461 gcgagaattc tggtcgttgc cgttatttgt ttcgccagcg accttaattc cgcgcccgga
     8521 tacggagtgt ctggtggagc aggcactggc gcggttgcct gaacaacctt gccgtattct
     8581 cgatctcggg acgggtaccg gggcgattgc gctggcgctg gctagcgagc gcccggactg
     8641 cgaaattacc gctgtagatc gtatgcccga tgctgtctct ctggcgcaac gtaatgccca
     8701 gaatctggcg atcaaaaaca tccatattct gcaaagcgac tggtttagcg cgctagccgg
     8761 gcagcagttt gcgatgattg tcagtaatcc gccgtatatt gacgagcagg acccacatct
     8821 tcaacaaggc gatgttcgct ttgagccgct cactgcgctg gttgcggcag acagtggaat
     8881 ggcagacatc gtgcatatca tcgaacagtc gcgtaacgcg ctggtatccg gcggctttct
     8941 gcttctggaa catggttggc agcagggcga agcggtgcga caggcattta tcctcgcggg
     9001 gtatcatgac gtcgaaacct gtcgggacta tggtgataac gagcgcgtaa cgctcggccg
     9061 ctattatcaa tgacaagttt ttctacactg cttagcgttc atctcattag cattgcgctt
     9121 tctgtagggc tattaacctt acgtttctgg ctacgttatc agaagcatcc acaggcattt
     9181 gcgcgctgga cgcgcattgt gccgccggtt gtcgatacgg tgttattgtt aagcggcatt
     9241 gcgttgatgg ctaaagcgca catcctgcca ttttccgggc aggcacagtg gctgactgaa
     9301 aagctgtttg gagttatcat ttatatcgtt ttgggtttta ttgcattcga ttatcgtcgt
     9361 atgcacagtc agcaggcgcg cattattgcc ttcccgctgg cgttggtggt gctgtacatc
     9421 atcattaaac tcgccaccac aaaagtaccg ttactggggt aagtcatgag atcgttagct
     9481 gatttcgaat ttaataaagc gccattgtgc gaaggcatga tcctggcttg cgaagcaatc
     9541 cgccgcgatt ttccctcgca agatgtttac gacgaactgg agcgtctcgt tagtctggcg
     9601 aaggaagaaa tcagccagct tctgccttta gaagagcagt tggaaaaact gatcgcgctg
     9661 ttttacggcg actggggatt taaagcctca cgcggcgttt atcgcctttc cgatgcatta
     9721 tggctggacc aggtgttaaa gaatcgacag ggcagtgcgg tatcattagg tgccgtttta
     9781 ttatgggtcg cgaatcgtct cgatttgccg ctattgccgg tgattttccc gacgcagctg
     9841 atattgcgca ttgaatgtcc ggatggcgaa atttggctga ttaatccttt taacggtgaa
     9901 tcgttaagcg aacatatgct ggatgtatgg ttaaagggaa atatcagccc gtcggcggaa
     9961 ctgttttatg aagaccttga tgaagctgat aacattgagg taatccgcaa attgctggat
    10021 acactcaaag cttcgttgat ggaagaaaat cagatggagc tggcgttacg caccagcgaa
    10081 gctttattac aattcaaccc ggaagatccc tatgaaattc gcgatcgcgg gttgatttat
    10141 gcgcaactgg attgcgaaca cgttgcgttg aacgatttaa gttatttcgt tgaacagtgt
    10201 ccggaagacc cgatcagcga aatgatccgt gcgcaaataa ataacatcgc gcataaacat
    10261 attgtgctgc attaattaat cgacatttta ctcaagatta aggcgatcct atgaaacaaa
    10321 aagtggttag cattggtgac atcaacgtag caaatgacct gccgttcgta ctgtttggcg
    10381 gtatgaacgt gctagaatct cgcgatctgg cgatgcgcat ttgcgagcac tacgtaaccg
    10441 tgacccagaa actgggtatc ccttacgtgt tcaaagcctc ttttgacaaa gccaaccgct
    10501 cctccatcca ctcttatcgt ggaccgggcc tggaagaagg gatgaaaatc ttccaggagt
    10561 tgaagcagac ttttggcgtg aaaattatca ccgacgttca cgaaccaagc caggctcagc
    10621 ccgttgctga tgttgtggat gtgattcagt tgccggcgtt tcttgctcgc cagactgacc
    10681 tggttgaagc catggcgaaa accggtgcgg taattaacgt caagaaaccg cagtttgtca
    10741 gcccgggaca gatgggtaat atcgttgata aattcaaaga aggcggcaac gaaaaagtga
    10801 ttctttgcga tcgcggtgct aacttcggct atgacaacct ggttgttgat atgctgggct
    10861 tcagcatcat gaagaaagtg tctggtaatt caccggtgat tttcgacgtg acccacgcac
    10921 tgcaatgccg cgatccgttt ggcgcagctt ccggcggtcg tcgtgctcag gtggctgagc
    10981 tggcacgtgc cggtatggcg gtaggtctgg cggggctgtt tattgaagcg catccggatc
    11041 cggaacatgc gaaatgtgat ggtccatccg cgctgccgct ggctaaactg gaaccgttcc
    11101 tcaagcagat gaaagcgatt gatgatctgg tgaaaggttt cgaagaactg gataccagca
    11161 agtaatcttt tttgcttgaa aactaaagcc ttagcgttct gcgttaaggc ttttttgtgc
    11221 acagaaaacc cccagctagg ctgggggttc cggaaagctt tcagctttaa gccagttatt
    11281 aaaacccctt ttgatttgtt aaaacacctt gcggtctggc aactgcaagt gtcaaacaag
    11341 aaatcaaaag ggggtcccaa tggggaacga aaagagctta gcgcacaccc gatggaactg
    11401 taaatatcac atagtttttg cgccaaaata ccgaagacag gtgttctaca gagagaagcg
    11461 tagagcaata ggcagtattt tgagaaagct gtgtgagtgg aaaagtgtac ggattctgga
    11521 agctgaatgc tgtgcagatc atatccatat gcttgtggag atcccgccca aaatgagcgt
    11581 atccggcttt atgggatatc tgaaagggaa aagcagtctg atgctttacg agcagtttgg
    11641 tgatttgaaa ttcaaataca ggaacaggga gttctggtgc agagggtact acgtcgatac
    11701 ggtgggtaag aacacggcga agatacagga ttacataaag caccagcttg aagaggataa
    11761 aatgggagag cagttatcga ttccctatcc gggcagcccg tttacgggcc gtaagtaacg
    11821 aagttggatg caaatgtaag atcgtgtgcg cctgttaggg cgcggctggt aagagagcct
    11881 tataggcgca tttgaaaaac ctccggctat gccggaggat attttttcat ggggccggat
    11941 acaaaaaagg ccgcaggctg ttactcctgc ggccggtttc gggcgcatat tgccatcacg
    12001 gcagcctgac gcccgttttc accttacttc cggttacgcc accagctgac aatcgctgcg
    12061 gtaataattc ccgccaggat cggtgctgcc aagtcgtgcc agaaagtcat ggcaaactgc
    12121 gtgagcgtca tatagccgcc ttgttgtaat gacaacattt tgcggctatt cttgaattgt
    12181 tctggttcaa gattagcccc cgttctgttg tcaggttata cctctcaacg tgcgggggtt
    12241 ttctctttcc agcaaccaat gccaccaggg atagagcccc cgcaacattg cgcctcaccg
    12301 gataacgccg gcttggtgtg gatactacga ctcaattcat cttcacttca tccctgaaat
    12361 gtatgcaatg aagagtgcat tccggttttt caacagctgt tacagtcgtt tcatgagtgc
    12421 tctggatgat gcttccagct cgggttgcca atatttactt gtggaagaga taaagacaaa
    12481 aaaggccgca ggctgttacc cctgcggccg gtttcgggcg catattgcca tcacggcagc
    12541 ctgacgcccg ttttcacctt acttccggtt acgccaccag ctgacaatcg ctgcggtaat
    12601 aattcccgcc aggatcggtg ctgccaggtc gtgccagaaa atcatggcaa actgcgcgag
    12661 cgtcatatag ccgccttgtt gtaatgacaa cattttgcgg ctattcttga agtgtctggt
    12721 ttcaagatta gcccccgttt tgttgtcagg ttttacctct caacgtgcgg gggttttctc
    12781 tttccagcaa ccaatgccac cagggataaa gcccccgcaa cattgcgcct caccggataa
    12841 cgccggcttg gtgtggatac tacgtctcaa ttcatcttca cttcatccct gaaatgtttg
    12901 caataaagag tacattccgg cttttcaaca gctgttgcag tggtttcgtg caggttctgg
    12961 tacaggctcg caattctgat taacaacctg ttcaggtagt atgaagaaat acaataagca
    13021 ggaatttata ttatctcccc cgatttctgt catcggagga gatataactg tcaggcaaat
    13081 atcgtcatca aataggcggc aaacagtgcc agatgcgctg cgccattgag cacgttagta
    13141 cgtccggtgg agaaggagat atggcacagc actaaagagg ccaccatcac taccatttct
    13201 ggcgcaccaa gtgcaaactg caattcgtta cccgtcataa aggcaattag cgtgacgaca
    13261 ggtacggtaa gcgaaatggt tgctaacact gaaccaaaga acagattcat cgcgcgctgg
    13321 acctggttgt tcaacactgc ttttaacgca cctaaacctt ccggcgacag aatcaacaga
    13381 gccaccagga agccagtaaa ggcgacaggg gcattcatgc tgtcgagcaa tgtctccagc
    13441 gggctggcgt tcattttggt gactgcaata acggcaatca gatggacaat caaccagata
    13501 gcatgccaaa ggctgctatg ggcagacggt ttaccgtggt gcgggtcgtc atcatcactg
    13561 tcatcttcgt gctcgtagac aaataaactt tgatgcgttt tggtctggat cagcaaaaat
    13621 acgccataca ttgccgcaga aattaatgct accagtaacg cctgaccggt tgaaaaattc
    13681 gccgcaggca gagccattgg aaataccagt acgattatcg ccagggggaa cagcgcaatt
    13741 aaatactgct tgataccaaa cagattcata tattgggtgg caaacttgcg tccgcccaac
    13801 aataatgaaa agccaaccag cccaccggta acaatcataa tgattgaata gagcgtatca
    13861 cgcattagcg ttggcgcggc gtcgccggtt gccattaaag ctgaaatcaa actgacttca
    13921 agaataacca ctgaaaggct aagaataagc gaaccgtaag gttctcccag gcgatgggct
    13981 aatacgtccg catgacggac aacactaaag gcgctactta aaataccaat aagagcaaga
    14041 agattgatgc caatgaccac tggtagtgtc tggctgcttc cccacaggaa cagcactacc
    14101 agcgccagaa ccgggaaaat aagcgaagtc tccttgtggc gggtttttac cgcctcttga
    14161 gcatttgaca ttatggttat ccctttgcag atgaatttat cgaaaatgta aaaaataggt
    14221 aggaaaaata acagaaagtg tctggatatc ggtaacattt tacgaatttt taccccgctg
    14281 tcgatttttt actcatcggg ccataaaaat aagcaatgcg tttagacaat gtttgtttta
    14341 gcgtttctta agaagagtct gacatgaaaa ttcttatgtt ttggcaagag tggatattgt
    14401 tgaccacact taatgttcaa ctttgtaaaa ggagtcaacg atgccgtata aaacgaaaag
    14461 cgatctgccg gaaagcgtaa agcacgtgct accgtctcat gctcaggata tctataaaga
    14521 agcgttcaac agcgcatggg atcaatataa agataaagaa gatcggcgtg atgacgccag
    14581 tcgcgaagaa accgcgcata aagtggcctg ggctgctgtg aagcatgaat atgccaaagg
    14641 ggatgatgat aaatggcata aaaaatcgta aaaccggtag cttagttaaa gcaattcgtg
    14701 cggtgttgcc ttgcaagtgg tccgtggatt gcatattgtc ccgttagtgg tttcaaaatg
    14761 agcagtaaaa atgtccggaa gacaccaaaa agttgtcgca gggaagtatg cagtggcgga
    14821 ggtgtaaggt gataacgcgt gatttcttga tgaatgccga ttgtaaaacg gcatttggtg
    14881 ccattgaaga atcactctta tggtcagcag agcaacgggc ggcttcgctg gcggcgacgc
    14941 tggcttgtcg acctgatgag ggaccggtgt ggatcttcgg ttatggatcg ttgatgtgga
    15001 atccggcact ggagtttacc gagtcgtgca ccggtacact ggttggatgg catcgtgcgt
    15061 tttgcctgcg tctgaccgcc gggcgcggga ctgcgcacca gccgggacga atgcttgcac
    15121 tgaaagaggg cggacgcacc acaggcgtcg cctatcgact gccagaagag acgctggagc
    15181 aggaactaac cctgttgtgg aagcgcgaga tgattaccgg ctgttatctg ccaacctggt
    15241 gtcagcttga tcttgatgat ggacgcacag taaacgccat tgtgtttatt atggacccgc
    15301 gacatccaga atatgaatct gacactcgcg ctcaggtcat cgcgccgttg attgcggcgg
    15361 cgagcggtcc actgggaacc aacgcacaat acctgttttc actggaacag gagcttatca
    15421 aactgggaat gcaggacgat gggctgaatg atttgctggt atcggtaaaa aaactgctgg
    15481 cggagaatta tccggatggt gtgttacggc cgggattcgc ctgagtaaac ttccctcata
    15541 gtggggcgtc agacgccccc tcaaacacta aaatgtgagc actttatcgg ctgacagcgt
    15601 ccattgcgcc agttccacaa gcgtaccgat ttccacccca tcaatcagag gaagtgtgct
    15661 aatcccgcgt ccgtcggtac aggttttgca caatttcacc ggtacattct gagcggtaag
    15721 gatctccagc atttgctgaa tgttgtagcc ttcccctggt ttttgcccgc gcaacccggc
    15781 tgtgaccgca tcagacatca ggaacaggcg cagatccaga ttgctctcct gctctcgtaa
    15841 cgcaatggcc agccgcaagc tgttaaacaa ggattcgctc ccgtaaggtg cgccattggc
    15901 aacgatcacg attttttgca ttatttactc ctgtattcag ggaattagac actcatcttc
    15961 tatcttactg cttctgcagc gtctgaccaa tcggtcacat ttttaaggat tttcctgaaa
    16021 gcgcgagaaa atacgacaaa agttgccagt aatcgttatt ctttagggct atggtttttc
    16081 attttttacc ggaagttacc gacgttttga gccgtttcgt tcctcgcatt attccgtttt
    16141 atttactctt gctggcggca ggcggtacag ctaacgcaca atctaccttc gagcaaaaag
    16201 cggcaaatcc ctttgataat aacaatgatg gtctgccgga tttaggcatg gcacctgaaa
    16261 atcatgatgg ggaaaaacac tttgctgaaa ttgtgaaaga tttcggcgaa accagtatga
    16321 atgataacgg gctggatact ggcgagcagg caaaagcttt cgcattggga aaagtccgcg
    16381 atgcgcttag tcaacaggtt aatcagcacg tagagtcctg gctatcaccg tggggaaatg
    16441 ccagtgttga cgtcaaagtg gataacgaag gacatttcac cggcagtcgt ggaagctggt
    16501 ttgtgccgtt acaagataat gatcgttatc tcacctggag ccagcttggt cttactcagc
    16561 aggatgatgg gctggtgagc aatgtgggcg ttgggcaacg ctgggcgcgc ggcagctggc
    16621 tggtgggtta taacactttt tatgacaact tgctggacga aaatcttcag cgagcgggct
    16681 tcggtgccga agcgtggggc gaatatttgc gactatcggc aaacttttat cagccgtttg
    16741 ctgcatggca tgaacagaca gccacgcagg aacaacggat ggcgcgcggg tacgacctga
    16801 cagcccggat gcgcatgccg ttttatcaac acctcaatac cagtgtcagc gtagaacagt
    16861 attttggtga tcgtgttgat ttgtttaact ctggtacggg ttatcacaat cccgtcgcgt
    16921 tgagtctggg attaaattat accccagtgc cattagtcac tgtgacggcc cagcataaac
    16981 agggtgaaag tggcgagaat caaaataacc tcgggctgaa tcttaattac cgctttggtg
    17041 taccgctcaa aaaacaactt tctgcgggcg aggttgccga aagtcagtcg ttacgtggta
    17101 gtcgctatga caatccgcag cgaaataatc ttccgactct tgagtaccga cagcgaaaaa
    17161 cgttaacggt gtttctggcg acaccgccgt gggatctaaa acctggcgaa acagtgccgc
    17221 tgaaattaca aatccgcagt cgttatggta ttcggcaact gatttggcag ggcgatacgc
    17281 agatattaag tttaacgcca ggcgcacaag ccaacagcgc ggagggctgg acgctgatca
    17341 tgcctgactg gcaaaacggg gaaggggcaa gcaatcactg gcgattgtcg gtggtggtgg
    17401 aagataacca ggggcagcgt gtctcctcca atgagatcac gctaacgctt gtcgaaccgt
    17461 tcgacgcatt gtcaaacgac gaactgcgct gggaaccgta atcagaaaat gcgctcctga
    17521 tgcacccata ccgctgcttc cacgcgagac ttgagcttca ttttcttcag catgtgcttg
    17581 acgtgcactt ttactgtgct ttcggtgatg tccaggcggc gggcaatcat cttgttcggc
    17641 aaaccctggg caatcagctt gagaatatcg cgctcgcgtg gggttaactg gttaacatcg
    17701 cgctcagtag tggcacggtt agcgcgcaag ctggcggcca gaacaggcgt taatgcttcg
    17761 cttaatacca tttcgccagc agctgcctga tgcaatgctt tcagcagatc ttccggttcc
    17821 atatctttta acagatagcc atccgcgccg cgtttcagtg cggtgaccac atcttcttca
    17881 tggttagaga cgctgaatac cacaatgcgc cctgagaggg acttttcgcg gagtttatcc
    17941 agcgtttcca gaccattcat gccgggcata ttgagatcta acaggatcag atcgggatca
    18001 agagattccg ccagttcaat accctgttcg ccattactcg cttcgccaac cacgctgata
    18061 tctggtgcca tactgataag ctgttttacg ccagttcgca gcatcgggtg atcgtcgatc
    18121 agcaggatag tagccggttc ctgattactc atgggtatct ccttggacgt ctgtgaaagt
    18181 tttttcggga ataaaggtga caaccacttc ggtgccacct gattcacgac ggcggacgcg
    18241 gcaatcgcct cgtaaacttt gcgcacggtc gcgcattatt atcatgccgt agtgattgct
    18301 gcggatggca ttttcaggca cgccgcagcc gttatcctgg acggtcagtt tgacctgatt
    18361 atcgttttgc gccaccgtca ccaagacctc actcgcttgc gaatgtttga gggcgttact
    18421 taatgcctca cgggcaattt gcaacaagtg gattgcctga tgcgaaggca ccagacgagg
    18481 tggcaattga taatccagct tcaccgggaa gccaaatttg gcgctgtact cttcgcaact
    18541 cgcctccagc gctggacgta atccaggctc ggtgagctgc aagcggaatg tggtgagcaa
    18601 ttcacgcaac tgcgcccagg atgcattcag ttcgttacgg atctgactta acagttcgcg
    18661 gctgctttct ggcagcgcat cgccctgcat ctgtaaacaa ctcacctgca tcttcatgca
    18721 agagagagat tgggcaatag aatcatgcag ttcgcgcgca atggtggcac gctcttccat
    18781 cacgatcaac tgttgctgac gttcctgatg gcgatccaac gccagcgtgg cggtgagttg
    18841 ttcaaccagg gtatccacca gttgttgttg atcatggcta agatgacgcc cctgcggtag
    18901 ggtcgccagc aaaatgccgt actgcgtatg agagtcagcc agccgccact tcagggttgt
    18961 gccgcgatcg ccaacgggta atacgccgcg cgggcagagc tggcagcctt tatcatcaca
    19021 agtcatatct ggctggcagg taaactcctg atgattctct tcatcatcag tgtcatacac
    19081 ccgcagttcg atatcacgta gcagggtcaa attctgtaag ccgttgagta caggtgacag
    19141 gcgttcacac agcggggcgc gggaatgcaa acggcggtta gcctgccata aaaaagagag
    19201 gatctgattt ttatgctcca gcccggcagt tttctcctga acccgctgct caagtacggc
    19261 ataactttcg gccagttctg cagacatatt gttcaatgca gttccaagca tcgccatttc
    19321 gttgcgcccg ctgatgtttg cgcgttgggt aaaatcgcga tggctgacgg cactcgccat
    19381 tgccagcagt tgccgccacg gttgtagcaa tcgcgcccgc aaccagataa tagtgaacac
    19441 cagtaaaagt gccataaata ccgccattac ccgatggacc agcaccactg tctcaatgcg
    19501 catttccgtg gtgcggtcaa aaccagatac cagctgatca agcccggcaa caaactggct
    19561 gacatccgct gacaccgttt cgcggttttg tgcacgcatc agcgtaggga tcagttcatt
    19621 acgccagtaa tcttgtaaac cctgtaattg cgccagttgt ccgtctcctt ctgctgctcg
    19681 ggttaactcg gcgctaaatg ccgtttgttc catttcttta attaagggct tgtctttctc
    19741 gcttaatggc actgctgcca acagacggta actttgcatg cgcagcgatc ccgctttgtt
    19801 gatcgcatgg gcgctgccct gaacgccttg caccagccag ccagaaaccg ccatccctgc
    19861 cagtccaata gcagtagaaa gcaacacaat aagcgcaacc tgattaacca gggtgagcgg
    19921 agagagacaa cgtttaagca tgtaaacctc ttccttcagg ctttaaatga gcaataacct
    19981 taatgaatgt gacgatacat tctggaatgg cagtattctc ggctattggc tgaagtatac
    20041 ccatacccgg aaagagttac tccttatttg ccgtgtggtt agtcgcttta catcggtaag
    20101 ggtagggatt ttacagcacc gtgaaaaatc tcataatttt ta
//
LOCUS       DACBVI010000078        20109 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.078, whole genome shotgun sequence.
ACCESSION   DACBVI010000078 DACBVI010000000
VERSION     DACBVI010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.078"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            276..947
                     /locus_tag="IU325_13705"
     CDS             276..947
                     /locus_tag="IU325_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HAP5810067.1"
                     /translation="MDKVLDSAILSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            957..2153
                     /locus_tag="IU325_13710"
     CDS             957..2153
                     /locus_tag="IU325_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP5810068.1"
                     /translation="MKTWIFICMAVAILLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFIAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWVGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSATGT
                     EKLSEA"
     gene            2131..2712
                     /locus_tag="IU325_13715"
     CDS             2131..2712
                     /locus_tag="IU325_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP5810069.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQTYLGAQVRTSNHRLDQQPVSVRTPEGIIATECDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2714..3487
                     /gene="yaiO"
                     /locus_tag="IU325_13720"
     CDS             2714..3487
                     /gene="yaiO"
                     /locus_tag="IU325_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HAP5810070.1"
                     /translation="MIKRTLLAAAIFSTLPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3675..3950
                     /gene="frmR"
                     /locus_tag="IU325_13725"
     CDS             3675..3950
                     /gene="frmR"
                     /locus_tag="IU325_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HAP5810071.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3985..5094
                     /locus_tag="IU325_13730"
     CDS             3985..5094
                     /locus_tag="IU325_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HAP5810072.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5187..6020
                     /gene="fghA"
                     /locus_tag="IU325_13735"
     CDS             5187..6020
                     /gene="fghA"
                     /locus_tag="IU325_13735"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HAP5810073.1"
                     /translation="MELIEKHASFGGWQNVYRHYSPSLKCEMNVGVYLPPKAASEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATARKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKEAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6146..6685)
                     /locus_tag="IU325_13740"
     CDS             complement(6146..6685)
                     /locus_tag="IU325_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 family protein"
                     /protein_id="HAP5810074.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6787..7998)
                     /gene="mhpT"
                     /locus_tag="IU325_13745"
     CDS             complement(6787..7998)
                     /gene="mhpT"
                     /locus_tag="IU325_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HAP5810075.1"
                     /translation="MSTRTPSSSSTRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILISSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8174..9187)
                     /gene="dmpG"
                     /locus_tag="IU325_13750"
     CDS             complement(8174..9187)
                     /gene="dmpG"
                     /locus_tag="IU325_13750"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HAP5810076.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGTHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9184..10134)
                     /locus_tag="IU325_13755"
     CDS             complement(9184..10134)
                     /locus_tag="IU325_13755"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HAP5810077.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVQYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10131..10940)
                     /gene="mhpD"
                     /locus_tag="IU325_13760"
     CDS             complement(10131..10940)
                     /gene="mhpD"
                     /locus_tag="IU325_13760"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HAP5810078.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQHDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNETIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(10950..11816)
                     /locus_tag="IU325_13765"
     CDS             complement(10950..11816)
                     /locus_tag="IU325_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxononadienedioate/2-hydroxy-6-
                     oxononatrienedioate hydrolase"
                     /protein_id="HAP5810079.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11834..12778)
                     /gene="mhpB"
                     /locus_tag="IU325_13770"
     CDS             complement(11834..12778)
                     /gene="mhpB"
                     /locus_tag="IU325_13770"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HAP5810080.1"
                     /translation="MYAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPATERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12780..14444)
                     /locus_tag="IU325_13775"
     CDS             complement(12780..14444)
                     /locus_tag="IU325_13775"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HAP5810081.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            14635..15468
                     /gene="mhpR"
                     /locus_tag="IU325_13780"
     CDS             14635..15468
                     /gene="mhpR"
                     /locus_tag="IU325_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HAP5810082.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLH
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            15545..16627
                     /locus_tag="IU325_13785"
     CDS             15545..16627
                     /locus_tag="IU325_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP5810083.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLPPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16750..19824
                     /locus_tag="IU325_13790"
     CDS             16750..19824
                     /locus_tag="IU325_13790"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HAP5810084.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDECWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGKNRLAVMVLRWSDGSYVEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDELRVTVSLWQGETQVASGTTPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPVLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEVPLDVAPQDKQLIE
                     LPELPQPESTGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSAPHAIPH
                     LTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADILADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSVQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            19876..>20109
                     /locus_tag="IU325_13795"
     CDS             19876..>20109
                     /locus_tag="IU325_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5810085.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLW"
BASE COUNT         4744 a         5491 c         5218 g         4656 t
ORIGIN      
        1 agaacatatt tttaatatgt aattaattga caaactaaat cacatctaaa tacaatatat
       61 aaaacaaaat aactatacaa accaaacaca caaacactta acatatgaac tacataaaag
      121 attacgcagc cgtatccccg tacctgaaag ccccttattt ttcagctatc agtaattttt
      181 aacacttaaa aattagcatc tgtctgtgcc agagattacg tctctgcggg catcgttgtg
      241 cttgtagtat aataaataaa atggagtgtc attaagtgga taaggtttta gattcagcca
      301 tcctttcttc ggcaaataaa agaaagggta tccttgctat tggcgcacat cctgatgata
      361 tagaattggg ctgtggcgcg tcgcttgctc gtcttgcgca aaaaggaatt tatatcgcag
      421 ccgtggtgat gactaccggc aactctggca cagatggaat aatcgatcgc catgaagaat
      481 cgcgcaacgc cctaaagata ttagggtgcc accaaactat tcatcttaat tttgctgaca
      541 cccgcgcgca tttacagctc aatgatatga tttccgccct ggaagatatc attaaaaatc
      601 aaattccttc tgatgttgaa atcatgcggg tatataccat gcatgatgcc gaccgccatc
      661 aggatcatct cgctgtttat caagcttcaa tggttgcctg ccgcactatt ccacaaattc
      721 tcggctacga aaccccgagt acctggcttt catttatgcc tcaggttttt gaatccgtta
      781 aagaagaata tttcacagtc aaacttgctg cattaaaaaa acataaaagc caggaacgac
      841 gcgattatat gcgtcatgat cgcctgcgtg cagttgcaca atttcgcggg caacaggtca
      901 atagcgatct gggtgaaggc tttgtaattc ataaaatgat tctttgagca aattgcatga
      961 aaacctggat atttatatgt atggccgtag caatattgct atggttcctg agtacgttaa
     1021 ggcgtaagcc cagccaaaaa aaaggttgta ttgacgccat tatacctgcg tataacgaag
     1081 gcccgtgtct ggcacagtca ctggataatc tactgcgtaa cccttatttt tgccgggtaa
     1141 tttgcgttaa cgacggctcc acggacaata ccgaagcggt catggcggaa gtcaaacgca
     1201 aatggggcga ccgctttatt gccgtcacgc aaaaaaatac cggtaaaggt ggtgcgctga
     1261 tgaatggcct caattacgcc acctgcgacc aggttttttt aagtgatgcc gacacctatg
     1321 ttccgcccga tcaagacgga atgggctata tgcttgcaga aatagagcgc ggtgctgatg
     1381 ccgtaggcgg cattccctct actgcgttga aaggcgcggg tctgttaccg cacatccgcg
     1441 cgaccgtaaa gttgccgatg attgttatga agcgcacgct acagcagctc ctgggcggcg
     1501 caccgtttat tatcagcggt gcctgcggga tgttccgtac tgatgtattg cgtaagttcg
     1561 gtttctctga tcgtactaaa gtcgaagacc ttgatctcac ctggacattg gtggcaaacg
     1621 gctatcgtat tcggcaggcg aatcgctgca tcgtataccc acaggaatgc aacagcccgc
     1681 gtgaggaatg gcgtcgctgg cggcgttgga ttgtgggcta cgcggtctgt atgcgcctgc
     1741 ataaaagact tttatttagc cgcttcggta tcttcagtat atttcctatg ctgttggttg
     1801 tgctttatgg cgttgggatt tatctcacta cctggtttaa tgaattcatc accaccgggc
     1861 cgcatggtgt ggtgttggca atgtttccgc ttatctgggt cggcgtagtt tgtgttattg
     1921 gtgcttttag cgcctggttt catcgttgct ggttgttggt gcctttagcg ccgctttccg
     1981 ttgtgtatgt attattagct tatgccatct ggattattta tggacttatt gcctttttta
     2041 ctggacgcga acctcagcgc gacaaaccca cccgctattc cgcactggtg gaagcgtcaa
     2101 ccgcttattc ccaaccttct gctacaggaa ctgaaaaact atctgaagct taatgttaaa
     2161 gagaaaaata ttcagattgc agaccaggta attattgatg aaactgcagg tgaagttgtt
     2221 atcggcgcga atacccgtat ttgtcacggt gccgttattc agggtccggt agtgattggc
     2281 gcaaactgcc tgataggtaa ttatgcgttt attcgtcctg gcacaataat cagcaatggc
     2341 gtaaaaattg gttttgccac cgaaattaaa aatgcggtta ttgaagcgga agcaacgatt
     2401 ggtccgcaat gctttatcgc cgactcggta gttgcaaacc agacatattt gggcgcacaa
     2461 gtacgtacca gtaatcatcg tctggatcaa cagcccgtat ctgttcgaac tccagaggga
     2521 attatcgcta ccgaatgcga taaattaggt tgttatatcg ggcagcgttc acgccttggc
     2581 gtacaagtta ttattttgcc tgggcgaatt atttccccga acacgcaact tggcccgcgc
     2641 gtgattgtag aacgtaattt acctaccgga acttattcac tccgacaaga acttatccgt
     2701 acaggagatt aaaatgataa aacggacgtt attagcggca gcaattttta gcacattgcc
     2761 cgcttatgcc gggttaactt ccattaccgc gggctacgat tttaccgatt attctggcga
     2821 tcatggcaac cgtaatttag cgtatgctga actggtggcg aaagttgaaa acgcaacgct
     2881 gctttttaat ctttcccagg ggcgtcgtga ttatgaaact gaacatttca atgccactcg
     2941 cggtcagggt gcggtctggt ataaatggaa taactggctg acaacccgaa cgggtattgc
     3001 ctttgcggat aatacgccgg tctttgcccg ccaggatttt cgtcaggata ttaacctggc
     3061 actgttgcca aaaacgcttt tcacgaccgg ttatcgctac acgaaatatt acgatgatgt
     3121 cgaagtcgat gcctggcaag gcggcgtatc actctatact ggcccggtga tcaccagcta
     3181 tcgctatacc cattatgact ccagcgatgc aggtggtagt tacagcaata tgatttccgt
     3241 gcgtctgaat gacccgcgcg gcactggtta tacgcaacta tggctaagcc gcggaacagg
     3301 tgcttacacc tatgactgga cgccagaaac acgctacggc agcatgaaga gcgtcagttt
     3361 gcaacgtatt caaccgctga ctgagcaact taatctcggg ctgacggcag gtaaagtgtg
     3421 gtacgacacc ccaaccgatg attataacgg tctgcaactt gcagcccatc tgacctggaa
     3481 attctgattc cttctgccgc ccgctatccg gggcggcctt ccctgccgat tagccccacc
     3541 ccctttcctc tttgttttcc gaccacattc accggataaa ttttattctc cagtgttata
     3601 tactataggg gggtatgcat tgacatatag aatacccccc tatagtatat tgcatgcaga
     3661 tgatgaggtg cgaaatgccc agtactccgg aagagaagaa aaaggtcctt actcgagttc
     3721 gtcgtattcg ggggcagatt gatgctctgg aacggtcgct ggagggtgat gccgaatgcc
     3781 gtgccatact ccaacagatc gctgccgttc ggggcgcggc taacgggctg atggcagaag
     3841 tgcttgaaag ccatatccgg gaaacgtttg accgaaatga ctgctacagc cgcgaagtca
     3901 gccaatccgt tgacgacact attgaactgg ttcgtgccta tcttaaatag ctgaatctat
     3961 taccatattg aggaagagcg agagatgaaa tcacgtgctg ccgttgcatt tgctcccggt
     4021 aaaccgctgg aaatcgttga aattgacgtt gcaccaccga aaaaaggtga agtgctgatt
     4081 aaagtcaccc ataccggcgt ttgccatacc gacgcattta ccctctccgg cgatgacccg
     4141 gaaggtgtat tcccggtagt tctcggtcac gaaggggccg gcgttgtggt tgaagtcggt
     4201 gaaggcgtaa ccagcgtcaa acctggcgac catgtgatcc cgctttacac cgcagagtgc
     4261 ggcgagtgtg agttctgtcg ttccggcaaa accaacctct gtgttgcggt tcgcgaaacc
     4321 cagggtaaag gcctgatgcc agacggcacc acccgttttt cttacaacgg gcagccgctt
     4381 tatcactaca tgggatgctc tacattcagt gaatacaccg tggtcgcgga agtgtctctg
     4441 gccaaaatta atccagaagc aaaccacgaa cacgtctgcc tgctgggctg tggcgtgacg
     4501 accggtattg gcgcggtaca caacacggct aaagtccagc caggtgattc tgttgccgta
     4561 tttggtcttg gcgcgattgg tctggcagtt gttcagggcg cgcgtcaggc gaaagcggga
     4621 cggattatcg ctattgatac gaacccgaag aaattcgatc tggcgcgtcg cttcggtgct
     4681 accgactgta ttaacccgaa tgactacgac aaaccgatta aagatgtcct gctggatatc
     4741 aacaaatggg gtatcgacca tacctttgaa tgcatcggta acgtcaacgt gatgcgtgcg
     4801 gcgctggaaa gtgcgcaccg cggctggggt cagtcggtga tcatcggggt cgcgggttcc
     4861 ggtcaggaaa tctccacccg tccattccag ttggtcactg gtcgcgtatg gaaaggttcc
     4921 gcgtttggcg gcgtgaaagg tcgttcccag ttaccgggta tggttgaaga tgcgatgaaa
     4981 ggtgatatcg atctggaacc gtttgtcacg cataccatga gcctggatga aattaatgac
     5041 gccttcgacc tgatgcatga aggcaaatcc attcgaaccg taattcgtta ctgattgccc
     5101 gcaggtttac cccgtccagt tcaggcgggg ttcttaatac tctccccggg cagccgtccg
     5161 ggggattaac cgtgagataa tgactgatgg aactcattga aaaacatgcc agctttggcg
     5221 gctggcaaaa tgtgtatcgg cattattccc catcactgaa atgtgaaatg aatgtcggcg
     5281 tctatctccc accaaaagcc gcaagtgaaa aattgccggt gttgtactgg ctttcagggc
     5341 tgacttgcaa cgagcagaat ttcattacta aatcggggat gcagcgttac gcggcggagc
     5401 acaacattat tgttgttgcg ccagacacca gtccgcgagg cagtcatgtc gcagatgctg
     5461 accgttacga tctcgggcaa ggtgccgggt tttacctgaa cgcaacgcaa gcgccgtgga
     5521 atgaacatta caaaatgtat gactatatcc gcaacgagct gccggattta gtgatgcaac
     5581 attttccggc aacggccaga aagtctatct caggtcattc tatgggcggg ctgggtgcac
     5641 tggtgctggc gttacgtaat ccagatgaat atgtcagcgt ctcggcgttt tcgcccattg
     5701 tctccccatc gcaagtaccg tggggacagc aagcctttgc cgcatatctt ggtgaaaata
     5761 aagaggcctg gttggattac gatccggtga gccttatttc acaaggtcaa cgtgttgcgg
     5821 aaatcatggt tgatcagggg ttgagtgatg atttttacgc agaacagctg cggactccaa
     5881 atcttgaaaa gatctgccag gagatgaata tcaaaacgtt aatccgttat cacgagggtt
     5941 atgatcacag ctattatttt gtctccagtt ttattggcga gcatattgcc taccacgcca
     6001 ataaactgaa tatgcgttaa taatagtgca cgactgccgg atgcggcgtg aacgccttat
     6061 ccggcctccg gatggcgcgg gaatttgtag gcatgataag acgcggcagc gtcgcatcag
     6121 gcatctgcgc acgacttatc tatgtttacc acatcaaatc atcaggcact ttgaagtcgg
     6181 catacggatc gtcttcatcc tgctcttcgg cgctgagcgc gctgtgcaac acaatactgc
     6241 tggcatcacg ctgagcaatt ttatcggcaa cgctggcggg aataatggcg tattcacctt
     6301 cgctattgtt atcgaccaac aagcgggcaa tcgccagacg accattaatc aactgcgctt
     6361 gcgtgagctt atcgacaaag attttcttaa tcagattacc gtcagtgaag ttaaaaccaa
     6421 tatcgccatt ggcaatggtg attcggttca tttcgataag ctgcttcacc tgagctttat
     6481 attctttcgc caacgccgct tgtttttgct gctcgctaag ctgtttatca cgctcaagct
     6541 gtgccttttt gttttcttct accgccgccc gagcttcacg cgcctgaaca cgcgattttt
     6601 tcgccgttct ctcgaccttc gccgcttttt tgctggtgac taatcctgct ttgagcaatt
     6661 gctcttgtaa ggtaagtttt gccatctttg tttccaaacc cgctgaataa tttttagcga
     6721 gtatacctgt aaacgccgta ggtgtattgg gggcaagtcc ctttcgcccc tacttaataa
     6781 ggcgcgtcag gcatcggcgc acggctgcat tcgtgatctc cggctcatca aaataaacac
     6841 tgccaggcca gcaacaagaa tacccggcgc agaagcggcc attacgccaa ccgtgccagt
     6901 gcctaatgcc agcattttcc cggccagtaa cggaccgctc atagccccca gacgccctac
     6961 cgccacggct gttcccacac ctgttgcgcg gatctgcgaa ctgtaaaaca acggtgccag
     7021 ggcatacaaa acgctttgcc cacctgtcgc aaacaacccc gcgacgaatc ccgccagcag
     7081 cataccgtta aatgacgaca ccgttcccag cgccagcagc gaagctaaca tgccgctata
     7141 aatcagtagc gacatggtta ctggacgcag cttatccatc aatgcgccca acattaacgt
     7201 cccgcttgcc gcccccattt gcagtgcaaa catcacccct gccgcctgcg atggctggaa
     7261 tccttgctcc accaaaagca gcggtagcca gttgatcaac atgtagacca ccagcagagt
     7321 gaagaaataa cacaaccaca gcagcagcgt cgcggttgcc gtttctggcg caaataacgc
     7381 acgcagtggt ggcgcagcct gtttttcgcc agcgaaaacc gccgactccg gcagccagcg
     7441 cattaatagt ggcaccagaa tcaacggcac tacaccaccg acccaaaaca ccgtttgcca
     7501 ggctaagttt gcccccgcga aacccagtgt cgccgccagc gccgcgccaa tggggacacc
     7561 gcaatacatc aggctcactg ccgtcccacg aaaacgtgga cccgcggctt cagacgtcag
     7621 ggcgatcaga ttcggcaacg ccgcccccag cccgacaccg gtcatcagtc gcgcaaagac
     7681 cagtgagggg aaatcccagg caatcgccgt tgccagtgag aacaaaccaa acagcgcaac
     7741 tgagctaatt aaaatacgct tacgaccata acggtccgcc agcattccgc caaccagcgc
     7801 gccgggtagc aaaccgagta ttccggcgct gaatatccag cccatttgca ttttatcgag
     7861 tgcgaaagcc tgggcgatgc cacccgccgc aatgccagcc gcctgaagat ccagtccttc
     7921 catcagagcg accaaaaaac aaagcccgat agtcagcatc aggcgggttg aagatgatga
     7981 aggggtacga gtcgacataa gatgaacctc gttgcgagtg caggtgaatc gcggctgata
     8041 cattgtcagc cgcgtaatca gaaccattta atgactgcct acgggcgaaa tataggccgg
     8101 ataaggcgtt tacgccgcat ccggcagtcg tgctgggatg ggcatacaaa atgcacgttc
     8161 tcggcagtca tcattatttg ttgttgcgca gatccagcgc cacgtcgacg atcatatcct
     8221 cctggccgcc aaccatccgg cgcttgccca gctcaacgag aatatccacc gcgcttaagc
     8281 cataacgcgc cgccgccgtt tcacagtgac gcaggaagct ggagtaaaca ccggcatatc
     8341 ccagcgccag cgtttcgcga tcgactcgta ccggacgatc ctgcaacgga cgcaccaggt
     8401 cgtcagcggc atccattaac gcatagagat cggtcccatg ctgccaaccc agtttatccg
     8461 cggcggcaat aaacacttcc agcggcgcgt tacctgcgcc cgcccccatt cccgcgagac
     8521 tggcatcgat tcggtcgcag ccctcttcca ccgccgcgat agagttcgcc acgccaagac
     8581 tcaggttatg gtgagcgtgc atgccggttt gcgtttctgg tttcagcact gctttcaggg
     8641 cgcggaaacg gtcacggata tcgctcatgt tcatcgcacc gccagaatcc accacataaa
     8701 tacaggtcgc accgtagcct tccatcagct ttgcctgctt tgcgagattc tccggcgtgg
     8761 tcatatggct catcatcaga aaaccaacgg tgtccattcc gagctcgcgg gcatactgaa
     8821 tatgctgggc ggaaacatcg gcttcggtac agtgcgttgc cacacgaacc acccgcgcgc
     8881 cagcctgcca ggcatttttc aaatcgtgaa tagtgccgat tccaggcagc aacaacgtcg
     8941 cgattttggc gtgcttcacc acatccgccg ccgcttcaat ccattcaagg tcgctatgtg
     9001 tgccgaaacc atagttaaag ctggacccct gcaaaccgtc gccatgggcc acttcaatcg
     9061 aatccacgcg ggcatcgtcc agtgctttgg caatctggcg gacattttcc agcgaatact
     9121 gatgacgaat ggcgtgcata ccgtcacgca acgtgacgtc cgagatataa agttttttac
     9181 cgttcatgcc gcttctcctg ccttgcgcgc cagtgactgg gccatttttt ccgctgtcgc
     9241 cagcgcactg gaagtcataa tgtcgaggtt gcccgcatag gcaggcagat aatgcgctgc
     9301 gccttcgact tccagccaga ccgccgtttt cagcccggag aactgcccca cgcctggtaa
     9361 attgaccggt ttatcctgcg ggatgacttc aaactgcacg cgctgtttca ggcgataacc
     9421 cggtacgtaa gcctgcaccg cctcagccat ttcattgatt gaggcttcga tatcatcttg
     9481 tgaagcttca tcgctcaata catacaccgt gtcacgcatc atcaacggtg gctctgctgg
     9541 gttaagcaca ataatcgcct tcccttttgc cgcgccaccc accacttcaa tggcgcgcga
     9601 agtggtttcc gtaaattcat cgatattggc acgcgtgcca ggtccggcag atttactggc
     9661 aatagaagcg ataatttcgg cgtattgaac acgcgccacg cgtgaaactg ccgccaccat
     9721 tggaatggtg gcctggccgc cgcaggtgac catgttgacg ttcagttgat cgacattcgc
     9781 ctcgagatta acgaccggca cgcagtaggg gccgatggca gcaggcgtca ggtcaattaa
     9841 gcgaatatcc ggtttcgctt cgcgcaaagc ggcatcgttt ttcacatgcg cacccgcgct
     9901 ggtcgcatca aatacaatgt cgatatcagc aaattcaggc atgttcatca gcccgatcac
     9961 cccttcatgg gtggtggcga cgcccatacg tctggcacgc gccagaccgt cggactgagg
    10021 atcaatgccg accatcaccg ccatctccag atgctgaccg tgacgcaaaa ttttaatcat
    10081 cagatcggtg ccaatgttgc cagaaccgat aatggcgact ttacgcttac tcatgacaga
    10141 cttccttttg gggctgcgct tgaaaatgtc gcagcaactg aacctatgcc ttcgatatgg
    10201 gcttcaaaac gatcgcccgc attcaccgcc accatcggac ctaatgcccc ggtaagaatg
    10261 atatctccgg cgcgcagcgg ttcacccagg ctggccattt tgcgtgccag ccagacggcg
    10321 gcattaagcg gatgtcccag gcattcgctg ccgcgcccgc tggaaacctc ttcgttatta
    10381 cgcgtcatct tcatggcgca gtttttcagg tctaaccccg ccggacgttg cgccggaccg
    10441 ccgatgacat acactccaca ggaggcgtta tctgccacgg tatcgacaaa ctgaatcgac
    10501 cagtcgcgaa tgcggctccc caccacttcc agcgccggaa gtacccattc aatggcgtta
    10561 tacaattcgt cgaaggtgat atcggttgcg ggtagatcgc ggttcaacac cagtgcaatc
    10621 tccgcttcaa tgcggggttg cagaacacgg gaaaaaggaa tggtttcgtt atcgccataa
    10681 cacatgtcgg caaataatgt cccgaaatcc ggttgatcaa cgcccagttg ttgctgcact
    10741 ttcggatgcg tcagccccac tttgcgcccc accactcgcc gtccctgagc gacgtcatgt
    10801 tgcacattga tgtgctgaat ggcgtaggcc gcttcagcgt tatcgatacc aatcagatcg
    10861 cgcagcggcg caatcgcttc gccctgctct gcggcgcggc gtaaatccgc cgccagttgc
    10921 tcaagagtat gcttcgtcat gaccgttcct taagggcgtg cgaggaaatt cagcaccagt
    10981 tgattgaaag cgtcggcatg ttcccactgc gcccagtgac cacagtcgcg gaagatatgc
    11041 agttcagaac cggcaatgcc ggacagcaga cgcagacccg catccatcgg cacaaagcgg
    11101 tcgttgcgcc cccagacaat cagggtttgc gctttgattt ccgccagacg tgggccaaaa
    11161 tccgggaact gtttcggatt ggcttccagg ctcttaacga agttttccag gtgatcgcgg
    11221 cgcgacagca tattattcag gcgcgcttca aacagggcgt cggtcaaatc gctggtatca
    11281 aaaacgaaga tatccatcat cagcttcagg ttttcgatag tcggctgacg ataaagctga
    11341 ttcagtcgct taataccttc ggttggcatc ggcgtaaaca aactcatgcc gcccgtaccg
    11401 ccgcccatca gcaccagttt gccgacgcgc tccggccagt taagagtgaa cgccacagaa
    11461 ctgtggccgc ccatcgagtt gcccagcagg tggattttgg cgatatccag ttgatccacc
    11521 acgcttttca ggattcgcgc attaagatcc gatcgcgaac cactattaac gatcgaatcg
    11581 ctcttacccc aacccggaca atccagcagg atcacccgat agcccgcctc taccagcgga
    11641 tcgatattgc ggctgaagtt cgcccagcca gtagcacccg ggccggaacc atgcagcagg
    11701 acaacagttt cgtcgccttg tccgcagtca ttaaaatgga tgcgcagcgt tttacccgct
    11761 tcttctacat tcagaaaacg gctggtggcg gcttcggttt gtggctgata actcatcatc
    11821 ttctcctgca tattcagttc tctgttctgg cgcttaacga gccaaatccg gcaatccact
    11881 ccgggattgg gcggtaataa cgcccttcgc tacgccagtt gccaaacgca gaaatagcgg
    11941 caaaagcggc gacccaggtt ttgatttcat gtgtcgactt tccggcaatg gcggaaagct
    12001 cttcgttact gacggcatcc agttcctgta tgcgtccctg ctccagcaaa gtcatgaact
    12061 ggttatccca aatcgggttg agcggatgca gagttctctg atcctcaaca aacttctcag
    12121 cggcgctaat cacccgctgc tgacgcaatt cacgctcagt ggcgggtaaa tctttcccgc
    12181 tccccaacag acggtcgcgc atatgggcat cggctttcgc cagttctggc accggcggct
    12241 gatgggaaag cccaccggac ccaaggaaca gcacgcgttt attgagagtg ctggtgaaac
    12301 gtccaatggc ttcccccaac atgcgggtac gctggaaacc gggcagcggt gtggcgacac
    12361 cgttgatgaa cacaggcaga accggcacct tatccagccc accgagcagg aactccagcg
    12421 gctgggcgaa cccgtggtcc acctgcatac agtaagaaac ggcaagatcg atcccgctct
    12481 tcatgacggc atgcgcacag gcctccgcca gctccacagg cacgggcagc tctcctgccg
    12541 cactgccgaa atcaccaatt gccgtcgctc caacgcctaa acagaacggt ggcatcacgt
    12601 catagaaaaa gccgttgtag tgatctggcg caaacagcac caccagttca ggggagaaag
    12661 ccgcaatacg ctcgcgggcg ctggcaatca cgccattgac ctcatcgagc acctcttgcg
    12721 ccgggtcgac ataccccacc agcggcgagt gggaaagaca gtgaagataa gcgtacatat
    12781 caggctacct tttcgacaga aacgtcggca tcagggcggg tcagcgtcat caccgacgcc
    12841 agtttattca gggtcttgcc cagggtttgc ggaatggcgg tggcggcaac aaagcgatcc
    12901 gggcgcatca ccaccagcga agcattgtgt tgcgcgaacc agctacgcag gcgaccttgc
    12961 gtatcgccca cgcgtagtac gccgtcgtgg ttatcctgtg cggtatgaat ttgcacttcc
    13021 ggcaccacct gaatgaagcg tgtgcccaac gcgcgccact gctggatttg ctcgtcgctc
    13081 atcccccaca gtggattgca tccccagcca attaccgcga agttcgcgcc gatcgcgtta
    13141 tcgagcagcg tcacgtcgcc gttttccagc gtgactttcg gctgaataaa catcttgccg
    13201 accggagagt gcttcgcttc gccctcacgc accagcgcac cgccgtaata ttgcggcatc
    13261 ggcttgaagc gcatttcgag gaagtagcgt tttactggcg gcagataatt caacagccag
    13321 gaaacgccgt cacgtaacgt accctgccag cgtttcggtg gagccagcac gttgcccgcc
    13381 gtcacggaca ggtcaatcat cgctttggcg tgatcgcgac gttcttgttg ataggtatcg
    13441 agcagcgcat cgcgggcttt cccctggata accaacgcca gtttccatgc gaggttaaag
    13501 gcgtcgcgca taccactgtt atagccctgc ccctgccaca ccggcatgat gtgcgcggca
    13561 tcgcccgcca gcagtacgcg atcaatacga aaacgttgcg ccagtcgcgc gttgtgggtg
    13621 tagacacgct ggcgaatcaa ttcaacattg tccggattag gcagcacttt gcttaacagc
    13681 ttgcgcatat tttgcggctc acgcagctgt tcttcggttt ctcccggcat caccataaat
    13741 tcaaagcgac gtaccgcatg aggcagcgcg gcagaaacat acgggcgcac cggatcacaa
    13801 cacaaataga tatgcggcgt acttaacgga tcgttggcga tatctaccac aatccactga
    13861 tttggcgcag ttttaccttc aaacggcaca ttcagggtgc gacggacaaa acttgcccca
    13921 ccatcacagg ccaccagcca ctgggctttg accgtttccc gctgcccttc tgccgttttc
    13981 aggtgcaagg tcacttcgtc atcttgctgg ctgaaggcct ccagctcgcg ggaaaacagg
    14041 cagcgcacat tcggaaaacg cgacaatcct tccagcatca ccgcatcgac ctgaggctga
    14101 ataaaggcgt tacggcgcgg ccagccaaat tcatcggtca ttggctgaat atcagcaaaa
    14161 cagcgacctt ttggggtgag aaaacgcatc gcgtgccacg gcgtagtgtg cggcagaaca
    14221 ttatcgacca ggccaaccga ctgcatggtg cgcagcgcct cgtcatcaat accaatcgca
    14281 cgcgggtagt cgatcaactt atcgagtttc tccaccacca gcacgtcaat gcccatctgg
    14341 ccgagatagt tcgccatcat cagcccgacc ggaccggcac cagcgatcgc cacctgaacg
    14401 ctatggttaa cagcaggctg gatgtcaggg tgttgtattg ccatttcagt acctcacgac
    14461 tcggacaaaa tgccgttgcg cgcacagtac agcgcaactt attttgttaa aaacatgtaa
    14521 atgatttttt attgtgcgct cagtatagga agggtgtttt cggctacaat caaaacatgc
    14581 ccgaatgtgc accaggtgca ccacgttgtt ttaaatatat aaatgtcaat taatatgcag
    14641 aacaatgagc agacggaata caaaaccgtg cgcggcttaa cccgcggtct aatgttatta
    14701 aatatgttaa ataaacttga tggcggtgcc agcgtcgggc tgctggcgga actcagcggc
    14761 ctgcatcgca ccactgtgcg gcgactgctg gagacgctgc aggaagaggg atatgtccgg
    14821 cgtagtccct ccgatgatag ttttcgtttg accatcaaag tgcggcaatt aagcgaagga
    14881 tttcgtgacg aacagtggat ttctgcactg gcggccccgc tgctgggcga tctgttgcgc
    14941 gaagtggttt ggccaacaga tgtgtccacc ctggatgttg atgcaatggt ggtacgcgaa
    15001 actacccacc gtttcagccg cttatccttt caccgggcaa tggtcgggcg acggttaccg
    15061 cttctgaaaa ccgcctcggg cctgacctgg ctggcctttt gcccggaaca agaacgcaag
    15121 gaattaatcg aaatgttagc ctcccgcccc ggtgatgact atcaactggc acgggaaccg
    15181 ttaaagctgg aagccattct ggcgcgcgcg cgcaaagagg gttacggaca gaactaccgc
    15241 ggctgggatc aggaggagaa gatcgcctct attgccgtac cgctgcacag tgaacaacgg
    15301 gtgattggct gtctgaatct ggtgtatatg gcgagcgcaa tgaccattga acaggcagcg
    15361 gaaaagcatc ttccggcgct gcaacgggtg gcaaaacaga tcgaagaagg ggttgaatcg
    15421 caggctattc tggtggccgg acggcgaagc ggcgtgcatt tacgttgaca ccatcgaatg
    15481 gcgcaaaacc tttcgtggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt
    15541 gaatgtgaaa ccagtaacgc tatacgatgt cgcagagtat gccggtgtct cttatcagac
    15601 cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtaga
    15661 agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa
    15721 gcagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat
    15781 tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt
    15841 agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt
    15901 cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc
    15961 ctgcactaat gttccggctt tatttcttga tgtctctgac cagacaccca tcaacagtat
    16021 tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca
    16081 ccagcaaatc gcgctgttag caggtccatt aagttctgtc tcggcacgtc tgcgcctggc
    16141 gggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga
    16201 ctggagcgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc
    16261 cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga
    16321 gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag
    16381 ctcgtgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac
    16441 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
    16501 gcccgtctca ctggtgaaaa gaaaaaccac ccttccgccc aatacgcaaa ccgcctctcc
    16561 ccgcgcgttg gccgattcat taatgcagct ggcacgacaa gtttcccgac tggaaagcgg
    16621 gcagtgagcg caacgcaatt aatgtgagtc agctcactca ttaggcaccc caggctttac
    16681 actttatgcg tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
    16741 gatacagcta tgactatgat tacggattca ctggccgtcg tattacaacg tcgtgactgg
    16801 gaaaaccctg gcgttaccca acttaatcgc cttgcggcac atcccccttt cgccagctgg
    16861 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctaaatggc
    16921 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtgc
    16981 gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat
    17041 gcgcctatct acaccaacgt gacctatccc attacggtca atccgccttt tgttcccgcg
    17101 gagaatccga caggttgtta ctcgctcaca tttaatattg atgaatgctg gctacaggaa
    17161 ggccagacgc gaattatttt tgatggtgtt aactcggcgt ttcatctgtg gtgcaacggg
    17221 cgctgggtcg gttacggcca ggacagccgt ttgccgtctg aatttgacct gagcgcattt
    17281 ttacgcgccg gaaaaaaccg cctcgcggtg atggtgctgc gctggagtga cggcagttat
    17341 gtggaagatc aggatatgtg gcggatgagc ggcattttcc gtgacgtctc gttgctgcac
    17401 aaaccgacca cacaaatcag cgatttccat gttgccactc gctttaatga tgatttcagc
    17461 cgcgcggtac tggaggcaga agttcagatg tgcggcgaac tgcgcgatga gctgcgggtg
    17521 acggtttctt tgtggcaggg tgaaacgcag gtcgccagcg gcaccacgcc tttcggcggt
    17581 gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca cgctacgtct gaacgtcgaa
    17641 aacccggcgc tgtggagcgc cgaaatcccg aatctctatc gtgcagtggt tgaactgcac
    17701 accgccgacg gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg cgaagtgcgg
    17761 attgaaaatg gtctcctgct gctgaacggc aagccggtgc tgattcgcgg cgttaaccgt
    17821 cacgagcatc atcctctgca tggtcaggtc atggatgagc agacgatggt gcaggatatc
    17881 ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc gaatcatccg
    17941 ctgtggtaca cgttgtgcga ccgctacggc ctgtatgtgg tggatgaagc caatattgaa
    18001 acccacggca tggtgccaat gaatcgtctg accgatgatc cgcgctggct accagcgatg
    18061 agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt gatcatctgg
    18121 tcgctgggga atgaatcagg ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa
    18181 tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg gagccgacac cacggccacc
    18241 gatattattt gcccgatgta cgcgcgcgtg gatgaagacc agcccttccc ggcggtgccg
    18301 aaatggtcca tcaaaaaatg gctttcgctg cctggagaga cgcgcccgct gatcctttgc
    18361 gaatatgccc acgcgatggg taacagtctt ggcggcttcg ctaaatactg gcaggcgttt
    18421 cgtcagtacc cccgtttaca gggcggcttc gtctgggact gggtggatca gtcgctgatt
    18481 aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg cgatacgcca
    18541 aacgatcgcc agttctgcat gaacggtctg gtctttgccg accgcacgcc gcatccggcg
    18601 ctgacggaag caaaacacca gcagcagttt ttccagttcc gtttatccgg gcgaaccatc
    18661 gaagtgacca gcgaatacct gttccgtcat agcgataacg agctcctgca ctggtcggtg
    18721 gcgttggatg gcaagccgct ggcaagcggt gaagtgcctc tggatgttgc tccacaagat
    18781 aaacagttga ttgaactgcc tgaactaccg cagccggaaa gcaccggaca actctggctt
    18841 acggtacacg tagtgcaacc gaacgcgacc gcatggtcag aagccggaca cattagcgcc
    18901 tggcagcagt ggcgtctggc ggaaaacctc agcgtgacac tcccctccgc gccccacgcc
    18961 atcccgcatc tgaccaccag cgaaatggat ttttgcatcg agctgggtaa taagcgttgg
    19021 caatttaacc gccagtcagg ctttctttca cagatgtgga ttggcgatga aaaacaactg
    19081 ctgacgccgc tgcgcgatca gttcacccgc gcaccgctgg ataacgacat tggcgtaagt
    19141 gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct ggaaggcggc gggccattac
    19201 caggccgaag cggcgttgtt gcagtgcacg gcagatatac ttgccgacgc ggtgctgatt
    19261 acgaccgctc acgcgtggca gcatcagggg aaaaccttat ttatcagccg gaaaacctac
    19321 cggattgatg gtagtgttca aatggcgatt accgttgatg ttgaagtagc gagcgataca
    19381 ccgcatccgg cacggattgg cctgacctgc cagctggcgc aggtcgcaga gcgggtaaac
    19441 tggctcggat tagggccgca agaaaactat cccgaccgcc ttactgcggc ctgttttgac
    19501 cgctgggatc tgccattgtc agacatgtat accccgtacg tcttcccgag cgaaaacggt
    19561 ctgcgctgcg ggacgcgcga attgaattat ggcccacacc agtggcgcgg cgacttccag
    19621 ttcaatatca gtcgctacag ccaacaacaa ctgatggaaa ccagccatcg ccatctgctg
    19681 cacgcggaag aaggcacatg gctgaatatc gacggtttcc atatggggat tggtggcgac
    19741 gactcctgga gcccgtcagt gtcggcggaa ttccagctga gcgccggtcg ctaccattac
    19801 cagttggtct ggtgtcaaaa ataataataa ccgggcaggc catgtctgcc cgtatttcgc
    19861 gtaaggaaat ccattatgta ctatttaaaa aacacaaact tttggatgtt cggtttattc
    19921 tttttctttt acttttttat catgggagcc tacttcccgt ttttcccgat ttggctacat
    19981 gacatcaacc atatcagcaa aagtgatacg ggtattattt ttgctgctat ttctctgttc
    20041 tcgctattat tccaaccgct gtttggtctg ctttctgaca aactcgggct gcgcaaatac
    20101 ctgctgtgg
//
LOCUS       DACBVI010000079        20050 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.079, whole genome shotgun sequence.
ACCESSION   DACBVI010000079 DACBVI010000000
VERSION     DACBVI010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.079"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(215..1027)
                     /locus_tag="IU325_13800"
     CDS             complement(215..1027)
                     /locus_tag="IU325_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymethylenebutenolidase"
                     /protein_id="HAP5810086.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKRN"
     gene            1289..2050
                     /locus_tag="IU325_13805"
     CDS             1289..2050
                     /locus_tag="IU325_13805"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HAP5810087.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            2191..3618
                     /gene="rmuC"
                     /locus_tag="IU325_13810"
     CDS             2191..3618
                     /gene="rmuC"
                     /locus_tag="IU325_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HAP5810088.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQKIADRASKLYDKMRLFVDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            3713..4468
                     /gene="ubiE"
                     /locus_tag="IU325_13815"
     CDS             3713..4468
                     /gene="ubiE"
                     /locus_tag="IU325_13815"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HAP5810089.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            4482..5087
                     /gene="ubiJ"
                     /locus_tag="IU325_13820"
     CDS             4482..5087
                     /gene="ubiJ"
                     /locus_tag="IU325_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HAP5810090.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            5084..6724
                     /gene="ubiB"
                     /locus_tag="IU325_13825"
     CDS             5084..6724
                     /gene="ubiB"
                     /locus_tag="IU325_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HAP5810091.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            6803..7072
                     /gene="tatA"
                     /locus_tag="IU325_13830"
     CDS             6803..7072
                     /gene="tatA"
                     /locus_tag="IU325_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HAP5810092.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            7076..7591
                     /gene="tatB"
                     /locus_tag="IU325_13835"
     CDS             7076..7591
                     /gene="tatB"
                     /locus_tag="IU325_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HAP5810093.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            7594..8370
                     /gene="tatC"
                     /locus_tag="IU325_13840"
     CDS             7594..8370
                     /gene="tatC"
                     /locus_tag="IU325_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HAP5810094.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            8412..9194
                     /gene="tatD"
                     /locus_tag="IU325_13845"
     CDS             8412..9194
                     /gene="tatD"
                     /locus_tag="IU325_13845"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HAP5810095.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARALDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLFPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAAITDANVKTLFGIAF"
     gene            complement(9191..9679)
                     /gene="rfaH"
                     /locus_tag="IU325_13850"
     CDS             complement(9191..9679)
                     /gene="rfaH"
                     /locus_tag="IU325_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HAP5810096.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKMVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            9846..11339
                     /gene="ubiD"
                     /locus_tag="IU325_13855"
     CDS             9846..11339
                     /gene="ubiD"
                     /locus_tag="IU325_13855"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HAP5810097.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIISGDDVDLHRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDFQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            11385..12086
                     /locus_tag="IU325_13860"
     CDS             11385..12086
                     /locus_tag="IU325_13860"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HAP5810098.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLSELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(12277..13440)
                     /gene="fadA"
                     /locus_tag="IU325_13865"
     CDS             complement(12277..13440)
                     /gene="fadA"
                     /locus_tag="IU325_13865"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HAP5810099.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRQVRAEDLSAHLMRSLLARNPT
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     NREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDAQFGLATMCIGLGQGIATVFERI"
     gene            complement(13450..15639)
                     /gene="fadB"
                     /locus_tag="IU325_13870"
     CDS             complement(13450..15639)
                     /gene="fadB"
                     /locus_tag="IU325_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HAP5810100.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEAIMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKRAAVLGAGIMGGGIAYQSAW
                     KGVPVIMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            15829..17160
                     /gene="pepQ"
                     /locus_tag="IU325_13875"
     CDS             15829..17160
                     /gene="pepQ"
                     /locus_tag="IU325_13875"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HAP5810101.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            17160..17774
                     /locus_tag="IU325_13880"
     CDS             17160..17774
                     /locus_tag="IU325_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HAP5810102.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            17813..19264
                     /gene="trkH"
                     /locus_tag="IU325_13885"
     CDS             17813..19264
                     /gene="trkH"
                     /locus_tag="IU325_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HAP5810103.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            19276..19821
                     /gene="hemG"
                     /locus_tag="IU325_13890"
     CDS             19276..19821
                     /gene="hemG"
                     /locus_tag="IU325_13890"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HAP5810104.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMSSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKSTLK"
BASE COUNT         4657 a         5087 c         5481 g         4825 t
ORIGIN      
        1 attaatgttt gtttgagaat gggagttgat ggtaaatctc taaggtgtct gccagtgaag
       61 agactcactt atgactatta cttgccgact ttcttaaggt caaatagcgg agtcgtgctc
      121 tccaatcaat tcatactgat tattttttgg gggacagaga tggcagcaaa acgcactcgt
      181 tcactcacca tctcaaatat agactaagag gaaactaatt ccgcttccca ccatactgct
      241 taaaccactc cagcatacgc tgccagccat ctttcgcaga ttcggcatga tagctcgggc
      301 gataatcggc gttgaatgca tgcccggcat ccgggtagac gataatctct gctttcgcat
      361 tagcagcccg cagcgcctgg cgcatggttt caacgctctc ctgcggaatg ctgttatcct
      421 gaccaccata taagccgaga accggcgcgt taagatcggt tgcgatatca acaggttgtt
      481 tcggtgaatt cagcgactta tcgcctgtca gtttgccgta ccatgccacc gccgctttca
      541 gttgcgggtt atgcgcagca tacagccagg tgatacgtcc accccagcag aatccggtga
      601 tcattaaacg atgaacatcg ccgccgttgc gggaagccca actggcgaca tgatcgagat
      661 cggccagcac ctgcgagtca ggcacttttg ctaccagacc gctaagtaac gtggggatat
      721 cggcaaaatc attcggatcg ccttcgcgga agtaaagttc aggtgcgata gccagatacc
      781 cctccagcgc cagacggcga caaatgtcgc ggatatgttc atgcacgcca aaaatttcct
      841 gcactacaat gaccactggc agtgggccat cgctttgctt tggtctggca tggtaagcag
      901 gcatgttatc cccttgtgaa gggatagagg tgaagcccgc cacaatcgcg tcgtccaggg
      961 tctgaacgat ggtcgaagct agaggcgatg cagcaggtgc aaatccagat tgttgtgttg
     1021 ttgccatggt attctccgta cccttataaa aatgttgcgc aatgttaact atagtcagca
     1081 tgcaacaaat cacattgcct gaatcggctc atcttttatg cagtcctgca gaatgaagag
     1141 tgatttatgt gatttgcatc acttttggtg ggtaaattta tgcaacgcat ttgcgtcatg
     1201 gtgatgagta tcacgaaaaa atgttaaacc cttcggtaaa gtgtcttttt gcttcttctg
     1261 actaaaccga ttcacagagg agttgtatat gtccaagtct gatgtttttc atctcggcct
     1321 cactaaaaac gatttacaag gggctacgct tgccatcgtc cctggcgacc cggatcgtgt
     1381 ggaaaagatc gccgcgctga tggataagcc ggttaagctg gcatctcacc gcgaattcac
     1441 cacctggcgt gcagagctgg atggtaaacc tgttatcgtc tgctctaccg gtatcggcgg
     1501 cccgtctacc tctattgctg ttgaagagct ggcacagctg ggcattcgca ccttcctgcg
     1561 tatcggtaca acgggcgcta ttcagccgca tattaatgtg ggtgatgtcc tggttaccac
     1621 ggcgtctgtt cgtctggatg gcgcgagcct gcacttcgca ccgctggaat tcccggctgt
     1681 cgctgatttc gaatgtacga ctgcgctggt tgaggctgcg aaatccatcg gcgcgacgac
     1741 tcacgttggc gtgacggctt cttctgatac cttctaccca ggtcaggaac gttacgatac
     1801 ttactctggt cgcgtagttc gtcactttaa aggttctatg gaagagtggc aggcgatggg
     1861 cgtaatgaac tatgaaatgg aatctgcaac cctgctgacc atgtgtgcaa gtcagggcct
     1921 acgtgccggt atggtagcgg gtgttatcgt taaccgcacc cagcaagaga tcccgaatgc
     1981 tgagacgatg aaacaaactg aaagccaagc ggtgaaaatc gtggtggaag cggcgcgtcg
     2041 tctgctgtaa tcctcttctc ctgtctgaag gccgacgcgt tcggcctttt gtatttttgc
     2101 gtagcgcctc gcaggaaatg cctttccaac tggacgtttg tacagcacaa ttctattttg
     2161 tgcgggtaag ttgttgcgtc aggaggcgtt gtggatttct caatcatggt ttacgcagtt
     2221 attgcgttgg tgggtgtggc aattggctgg ctgtttgcca gttatcaaca tgcgcagcaa
     2281 aaagccgagc aattagctga acgtgaagag atggtcgcgg agttaagcgc ggcaaaacaa
     2341 caaattaccc aaagcgagca ctggcgcgct gagtgcgagt tactcaataa cgaagtgcgc
     2401 agcctgcaaa gtattaacac ctctctggag gccgatctgc gtgaagtaac cacgcggatg
     2461 gaagccgcac agcaacatgc tgacgataaa attcgccaga tgattaacag cgagcagcgc
     2521 ctcagtgagc agtttgaaaa cctcgccaac cgtatttttg agcacagcaa tcgccgggtt
     2581 gatgagcaaa accgtcagag tctgaacagc ctgttgtcgc cgctacgtga acaactggac
     2641 ggtttccgcc gtcaggttca ggacagcttt ggtaaagaag cacaagaacg ccataccctg
     2701 acccacgaaa ttcgcaatct ccagcagctc aacgcgcaaa tggcccagga agcgatcaac
     2761 ctgacgcgcg cgctgaaagg cgacaataaa acccagggca actggggcga ggtagtattg
     2821 acgcgggtgc tggaggcttc cggtctgcgt gaagggtatg aatatgaaac ccaggtcagc
     2881 atcgaaaatg acgcccgctc gcggatgcag ccggatgtca tcgtgcggtt gccgcaggga
     2941 aaagatgtgg tgatcgacgc caaaatgacg ctggtcgcct atgaacgcta tttcaacgcc
     3001 gaagatgact acacccgcga aagcgcgcta caggaacata tcgcgtcggt gcgtaaccat
     3061 atccgtttgc tgggacgcaa agattatcaa cagctgccgg ggctgcgaac cctggattac
     3121 gtgctgatgt ttattcccgt tgaacccgct tttttactgg cgcttgaccg ccagccggag
     3181 ctgatcaccg aagcgttgaa aaacaatatc atgctggtta gcccgaccac gctgctggtg
     3241 gcgctgcgca ctatcgccaa cctctggcgc tatgagcatc aaagtcgtaa cgcccagaaa
     3301 atagccgatc gcgccagcaa gctgtacgac aagatgcgtt tgttcgtcga tgacatgtca
     3361 gcgattggtc aaagtctcga caaagcgcag gataactatc gccaggcaat gaaaaaactc
     3421 tcttcaggtc gcggaaatgt gctggcgcag gcagaagcgt ttcgcggttt aggggtagaa
     3481 attaaacgcg agattaatcc ggatttggct gaacaagcgg tgagccagga tgaagagtat
     3541 cgactgcggt cggttccgga gcagccgaat gatgaagctt atcaacgcga tgatgaatat
     3601 aatcagcagt cgcgctagcc cattgggggt agttaagccg ggtagaaatc tagggcatcg
     3661 acgcccaatc tgttacactt ctggaacaat tttttgatga gcaggcattg agatggtgga
     3721 taagtcacaa gaaacgacgc actttggttt tcagaccgtc gcgaaggaac aaaaagcgga
     3781 tatggtcgcc cacgttttcc attccgtggc atcaaaatac gatgtcatga atgatttgat
     3841 gtcatttggt attcatcgtt tgtggaagcg attcacgatt gattgcagcg gcgtacgccg
     3901 tgggcagacc gtgctggatc tggctggtgg caccggcgac ctgacagcga aattctcccg
     3961 cctggtcgga gaaactggca aagtggtcct tgctgatatc aatgaatcca tgctcaaaat
     4021 gggccgcgag aagctgcgta atatcggtgt gattggcaac gttgagtatg ttcaggcgaa
     4081 cgctgaggcg ctgccgttcc cggataacac ctttgattgc atcactattt cgtttggtct
     4141 gcgtaacgtc accgacaaag ataaagcact gcgttcaatg tatcgcgtgc tgaaacccgg
     4201 cggccgcctg ctggtgcttg agttctcgaa gccaattatc gagccgctga gcaaagccta
     4261 tgatgcatac tccttccatg tgctgccgcg tattggctca ttggtcgcga acgacgccga
     4321 cagctaccgt tatctggcag aatccatccg tatgcatccc gatcaggata ccctgaaagc
     4381 catgatgcag gatgccggat tcgaaagtgt cgactactac aatctgacgg caggggttgt
     4441 ggcgctgcat cgtggttata agttctgaca ggagaccgga aatgcctttt aaacctttag
     4501 tgacggcagg aattgaaagt ctgctcaaca ccttcctgta tcgctcaccc gcgctgaaaa
     4561 cggcccgctc gcgtctgttg ggcaaagtgc tgcgcgtgga ggtaaaaggc ttttcgacat
     4621 cattgattct ggtgttcagc gaacgccagg ttgatgtact gggcgaatgg gcaggcgatg
     4681 ctgactgcac cgttatcgcc tacgccagcg tgttgccgaa acttcgcgat cgccagcagc
     4741 ttgccgcact gattcgcagt ggtgagctgg aagtacaggg cgatattcag gtggtgcaaa
     4801 acttcgttgc gctggcagat ctggcagagt tcgaccctgc ggaactgctg gccccttata
     4861 ccggtgatat cgccgctgaa ggtatcagca aagccatgcg cggaggcgca aagttcctgc
     4921 atcacggcat taagcgccag caacgttatg tggcagaagc cattactgaa gagtggcgta
     4981 tggcacctgg tccgcttgaa gtggcctggt ttgcggaaga gacggctgcc gtcgagcgtg
     5041 ctgttgatgc cctgaccaaa cggctggaaa aactggaggc taaatgacgc caggtgaagt
     5101 acggcgccta tatttcatca ttcgcacttt tttaagctac ggacttgatg aactgatccc
     5161 caaaatgcgt atcaccctgc cactacggct atggcgatac tcattattct ggatgccaaa
     5221 tcggcataaa gacaaacctt taggtgagcg actacgactg gccctgcaag aactggggcc
     5281 ggtatggatc aagttcgggc aaatgttatc aacccgccgc gatctttttc cgccgcatat
     5341 tgccgatcag ctggcgttat tgcaggacaa agtcgctccg tttgatggca agctggcgaa
     5401 gcagcagatt gaagctgcaa tgggcggctt gccggtagaa gcgtggtttg acgattttga
     5461 aatcaagccg ctggcttctg cttctatcgc ccaggttcat accgcgcgat tgaaatcgaa
     5521 tggtaaagag gtggtgatta aagtcatccg cccggatatt ttgccggtta ttaaagcgga
     5581 tctgaaactt atctaccgtc tggctcgctg ggtgccgcgt ttgctgccgg atggtcgccg
     5641 tctgcgccca accgaagtgg tgcgcgagta cgaaaagacc ttgattgatg aactgaattt
     5701 gctgcgggaa tctgccaacg ccattcagct tcggcgcaat tttgaagaca gcccgatgct
     5761 ctacatcccg gaagtttacc ctgactattg tagtgaaggg atgatggtga tggagcgcat
     5821 ttacggcatt ccggtgtctg atgttgcagc gctggagaaa aacggcacta acatgaaatt
     5881 gctggcggaa cgcggcgtgc aggtgttctt cactcaggtc tttcgcgaca gctttttcca
     5941 tgccgatatg caccctggca acatcttcgt aagctatgaa cacccggaaa acccgaaata
     6001 tatcggcatt gattgcggga ttgttggctc gctaaacaaa gaagataaac gctatctggc
     6061 agaaaacttt atcgccttct ttaatcgcga ctatcgcaaa gtggcagagc tacacgtcga
     6121 ttctggctgg gtgccaccag ataccaacgt tgaagagttc gaatttgcca ttcgtacggt
     6181 ctgtgaacct atctttgaga aaccgctggc cgaaatttcg tttggacatg tactgttaaa
     6241 tctgtttaat acggcgcgtc gcttcaatat ggaagtgcag ccgcaactgg tgttactcca
     6301 gaaaaccctg ctctacgtcg aaggggtagg acgccagctt tatccgcaac tcgatttatg
     6361 gaaaacggcg aagcctttcc tggagtcgtg gattaaagat caggtcggta ttcctgcgct
     6421 ggtgagagca tttaaagaaa aagcgccgtt ctgggtcgaa aaaatgccag aactgcctga
     6481 actggtttac gacagtttgc gccagggcaa gtatttacag cacagtgttg ataagattgc
     6541 ccgcgagctt cagtcaaatc atgtacgtca gggacaatcg cgttattttc tcggaattgg
     6601 cgctacgtta gtattaagtg gcacattctt gttggtcagc cgacctgaat gggggctgat
     6661 gcccggctgg ttaatggcag gtggtctgat cgcctggttt gtcggttggc gcaaaacacg
     6721 ctgatttttt catcgctcaa ggcgggccgt gtaacgtata atgcggcctt gtttaatcat
     6781 catctaccac agaggaacat gtatgggtgg tatcagtatt tggcagttat tgattattgc
     6841 cgtcatcgtt gtactgcttt ttggcaccaa aaagctcggc tccatcggtt ccgatcttgg
     6901 tgcgtcgatc aaaggcttta aaaaagcaat gagcgatgat gaaccaaagc aggataaaac
     6961 cagtcaggat gctgatttta ctgcgaaaac tatcgccgat aagcaggcgg atacgaatca
     7021 ggaacaggct aaaacagaag acgcgaagcg ccacgataaa gagcaggtgt aatccgtgtt
     7081 tgatatcggt tttagcgaac tgctattggt gttcatcatc ggcctcgttg ttctggggcc
     7141 gcaacgactg cctgtggcgg taaaaacggt cgcgggctgg attcgcgcgt tgcgttcact
     7201 ggcgacaacg gtgcagaacg aactgaccca ggagttaaaa ctccaggagt ttcaggacag
     7261 tctgaaaaag gttgaaaagg cgagcctcac taacctgacg cccgaactga aagcgtcgat
     7321 ggatgaacta cgccaggccg cggagtcgat gaagcgttcc tacgttgcaa acgatcctga
     7381 aaaggcgagc gatgaagcgc acaccatcca caacccggtg gtgaaagaca atgaagctgc
     7441 gcatgaaggc gtaacgcctg ctgctgcaca aacgcaggcc agttcgccgg aacagaagcc
     7501 agaaaccacg ccagagccgg tggtaaaacc tgctgcggac gctgaaccga aaaccgctgc
     7561 accttcccct tcgtcgagtg ataaaccgta aacatgtctg tagaagatac tcaaccgctt
     7621 atcacgcatc tgattgagct gcgtaagcgt ctgctgaact gcattatcgc ggtgatcgtg
     7681 atattcctgt gtctggtcta tttcgccaat gacatctatc acctggtatc cgcgccattg
     7741 atcaagcagt tgccgcaagg ttcaacgatg atcgccaccg acgtggcctc gccgttcttt
     7801 acgccgatca agctgacctt tatggtgtcg ctgattctgt cagcgccggt gattctctat
     7861 caggtgtggg cgtttatcgc cccagcgctg tataagcatg aacgtcgcct ggtggtgccg
     7921 ctgctggttt ccagctctct gttgttttat atcggcatgg cgttcgccta ctttgtggtc
     7981 tttccgctgg catttggctt ccttgccaat accgcgccgg aaggggtaca ggtatccacc
     8041 gacatcgcca gttatttaag cttcgttatg gcgttgttta tggcgtttgg tgtctccttt
     8101 gaagtgcctg tggcaatagt gctgctgtgc tggatgggga ttacctcgcc agaagactta
     8161 cgcaaaaaac gcccgtatgt gctggttggt gcattcgttg tcgggatgct gctgacaccg
     8221 ccggatgtct tctcgcaaac gctgttggcg atcccgatgt actgtctgtt tgaaatcggt
     8281 gtcttcttct cacgctttta cgttggtaaa gggcgaaatc gggaagagga aaacgacgct
     8341 gaagcagaaa gcgaaaaaac tgaagaataa attcaaccgc ccgtcagggc ggttgtcata
     8401 tggagtacag gatgtttgat atcggcgtta atttgaccag ttcgcaattt gcgaaagacc
     8461 gtgatgatgt tgtagcacgc gctcttgacg cgggagttaa tgggctactc atcaccggta
     8521 ctaacctgcg tgaaagccag caggcgcaaa agctggcgcg tcagtattca tcctgttggt
     8581 caacggcggg cgtacatcct cacgacagca gccagtggca agctgcgact gaagaagcga
     8641 ttattgagct ggccgcgcag ccagaagtgg tggcgattgg tgaatgtggt ctcgacttta
     8701 accgcaactt ttcgacgccg gaagagcagg aacgcgcttt tgttgcccag ctacgcattg
     8761 ccgcagaatt aaacatgccg gtatttatgc actgtcgcga tgcccacgag cggtttatga
     8821 cattgctgga gccgtggctg gataaactgc ctggtgcggt tcttcattgc tttaccggca
     8881 cacgcgaaga gatgcaggcg tgcgtggcgc atggaattta tatcggcatt accggttggg
     8941 tttgcgatga acgacgcgga ctggagctgc gggaactttt gccgttgatt ccggcggaaa
     9001 aattgctgat cgagactgat gcgccgtatc tgttccctcg cgatctcacg ccaaagccat
     9061 catcccggcg caacgagcca gcccatctgc cccatatttt gcaacgaatt gcgcactggc
     9121 gtggagaaga tgccgcatgg ctggctgcca tcacggatgc caatgttaag acactgtttg
     9181 ggattgcgtt ttagagtttg cggaactcgg tgttcttcac actgtgctta atctctttat
     9241 tgataagatt aagtaacagc atggagcgag cctcgccgtc gggttcggtg aaaatggcct
     9301 gaaaaccttc gaacgcgcct tcggtaataa tcaccttatc acccggataa ggggttgccg
     9361 gatcgacaat gtctttcggt ttatataccg atagctgatg aatcaccgcc gatgggacta
     9421 tcgctggcga tgcgccaaag cgcacgaagt ggctgacacc gcgggtcgcg ttgatagtcg
     9481 tggtatgaat cacttctgga tcaaattcca caaacaggta gttggggaac aatggttcac
     9541 tgactgcagt acgtttgcca cgcaccattt tttccagggt gatcatcggt gccaggcaat
     9601 tcacagcctg tctttcgagg tgttcctggg cacgttgaag ttgcccgcgc ttgcagtaca
     9661 gtaaatacca ggattgcata atgactctta tccgtttaat cggggcgcaa ggatagcaaa
     9721 agctttacgc taagttaatt atattccccg gtttgcgtta taccgtcaga gttcacgcta
     9781 atttaacaaa tttacagcat cgcaaagatg aacgccgtat aatgggcgca gattaagagg
     9841 ctacaatgga cgccatgaaa tataacgatt tacgcgactt cctgacgctg cttgaacagc
     9901 agggtgagct aaaacgtatc acgctcccgg tggatccgca tctggaaatc actgaaattg
     9961 ctgaccgcac tttgcgtgcc ggtgggcctg cgctgttgtt cgaaaaccct aaaggctact
    10021 caatgccggt gctgtgcaac ctgttcggta cgccaaagcg cgtggcgatg ggcatggggc
    10081 aggaagatgt ttctgcgctg cgtgaagtcg gtaaattatt agcctttctc aaggaacctg
    10141 aaccgccaaa aggttttcgc gacctgtttg ataaactgcc gcagttcaag caagtattga
    10201 acatgccgac aaagcgactg cgtggtgcgc cgtgtcagca aaaaattatc tccggcgatg
    10261 acgtcgatct tcaccgtatc cccattatga cctgctggcc ggaagacgcc gcgccgctga
    10321 ttacctgggg gttgaccgtg acgcgtggcc cgcataaaga gcggcagaat ctgggtattt
    10381 atcgtcaaca gttgattggt aaaaataaac tgattatgcg ctggttgtcg catcgcggcg
    10441 gcgcgctgga tttccaggag tggtgtgcgg cgcatccggg cgaacgtttc ccggtttctg
    10501 tggcgctggg tgccgatccc gccacgattc tcggtgcagt cacccccgtt ccggatacgc
    10561 tttcagagta tgcgtttgcc ggattgctgc gcggcaccaa aaccgaagta gtaaagtgta
    10621 tttccaatga tctcgaagtg cccgccagtg cggagattgt gctggaaggg tatatcgaac
    10681 aaggcgaaac tgcgccggaa gggccgtatg gcgaccacac cggttactat aacgaagtcg
    10741 atagtttccc ggtatttact gtgacgcata ttacccagcg tgaagatgcg atttatcatt
    10801 ccacctatac cgggcgtccg ccggatgaac ctgcggtgct gggtgtcgca ctgaacgaag
    10861 tgtttgtgcc gattctgcaa aaacagttcc cggaaattgt cgatttttac ctgccgccgg
    10921 aaggctgctc ttatcgcctg gcggtagtga caatcaaaaa acagtacgcc ggacacgcga
    10981 agcgcgtcat gatgggcgtc tggtcgttct tacgccagtt tatgtacact aaatttgtga
    11041 tcgtttgcga tgatgacgtc aacgcacgcg actggaacga tgtgatttgg gcgattacta
    11101 cccgtatgga cccggcgcgg gatactgttc tggtagaaaa tacgcctatt gattatctgg
    11161 attttgcctc gcctgtctcc gggctgggtt caaaaatggg gctggatgcc acgaataaat
    11221 ggccggggga aacccagcgt gaatggggac gtcccatcaa aaaagatcca gatgttgtcg
    11281 cgcatattga cgccatctgg gatgaactgg ctatttttaa caacggtaaa agcgcctgat
    11341 gcgcgtttgt tttgccctat ttatcgatcc gacagagaaa gcgcatgaca accttaagct
    11401 gtaaagtgac ctcggtagaa gctatcacgg ataccgtata tcgtgtccgc atcgtgccag
    11461 acgcggcctt ttcttttcgt gctggtcagt atttgatggt agtgatggat gagcgcgaca
    11521 aacgtccgtt ctcaatggcc tcgacgccgg atgaaaaagg gtttatcgag ctgcatattg
    11581 gcgcttctga aatcaacctt tacgcgaaag cagtcatgga ccgcattctt aaagatcatc
    11641 aaatcgtggt cgatattccg cacggtgagg catggctgcg cgatgatgaa gaacgtccaa
    11701 tgattctcat cgcgggcggc accgggttct cttatgcgcg ctcgattttg ctgacggcgc
    11761 tggcgcgtaa cccaaaccgt gatatcacca tttactgggg cgggcgtgaa gagcagcatc
    11821 tttatgatct ctccgagctt gaggcgcttt cgcttaagca tccaggtttg caagtggtgc
    11881 cggtggttga acaaccggaa gcgggctggc gtgggcgtac tggcaccgtg ttaacggcgg
    11941 tattgcagga tcatggtacg ctggcagagc atgatatcta tattgccgga cgttttgaga
    12001 tggcgaaaat tgcccgtgac ctgttttgca gtgagcgtaa tgcgcgggaa gatcgcctgt
    12061 ttggcgatgc gtttgcattt atctgagata taaaaaaccc gcccctgaca ggcgggaaga
    12121 acggcaacta aaactttata cagtggcatt tagatctatg tcgcatccgg cattcggcac
    12181 acgatgcctg atgcgacgct ggcgcgtctt atcaggccta cattggtgcc ggatcggtag
    12241 accggataag gcgttcacgc cgcattcggc aagtggttaa atccgctcaa acaccgtcgc
    12301 aataccctga cccagaccga tacacatcgt cgctagacca aactgggcat ctttgcgctc
    12361 catcagattc agcagcgtgg tgctgatacg cgcaccggaa caacccagcg gatgacccag
    12421 cgcgatcgcg ccaccgttga ggttgatctt ctcgtcaatc tgctcaatca gtcccagatc
    12481 tttaatacat ggcaggatct gcgcggcaaa ggcttcattc atttcaaaca cgccgatatc
    12541 actgacagaa agccccgctt ttttcagtgc cagcttcgag gccggaaccg ggccgtaacc
    12601 cataatcgat gggtcacaac caacgaccgc catcgaacgc acgcgggcgc gcggcttaag
    12661 acctaattca tgggcgcggc tttcactcat caccagcatg gcagctgcgc catcggaaag
    12721 cgcagaagat gtacccgccg ttaccgtacc gctgacggga tcaaacgccg gacgcagcgt
    12781 ggcgagggct tccacggtgg tttccgggcg aatcacttcg tcgtaattaa actgcttcag
    12841 gacgccgtcg gcatcgtgac caccggtcgg gataatttca tttttaaatg cacccgactg
    12901 cgtggcagcc caggcgcggg cgtgtgaccg cgcggcaaag gcatcctgca tttcacggtt
    12961 gataccgtgc atacgcgcca gcatttctgc cgttaagccc atcatgcccg ccgctttggc
    13021 gacattgcgg ctcaggccgg ggtgaaaatc gacgccgtga ctcatcggca catggcccat
    13081 atgctccacg ccgccaacca gacatgcctg cgcatcgccg gtcatgatca ttcgtgctgc
    13141 atcatgcagt gcctgcatgg atgaaccaca caagcgattg acggtaaccg ccgggacaga
    13201 gtgtggtact tctgccagca gcgccgcgtt acgggcgata ttaaaaccct gctccagcgt
    13261 ctgctgcaca caaccccagt aaatatcgtc gagggccgcc gcttccagcg tcgggttacg
    13321 cgccagcagg ctacgcatta aatgagcgga gagatcttct gcacgcactt gacgaaaagc
    13381 accgcccttc gaacggccca tcggggtgcg aattgcatcg acaatgacaa cctgttccat
    13441 tgtgactcct taagccgttt tcaggtcgcc aaccggacgg gctggctcaa ccgggggata
    13501 gtacggttcg ttatgacgcg ctttattacg cagaccttcc ggcacttcat acagcgggcc
    13561 gaggtgctga tattgctgtg ccatatcgag gtattttgcg ctaccgaggg tgtccagcca
    13621 gcggaacgcg ccaccgtgga acggagggaa gcccaggccg tagaccagcg ccatatccgc
    13681 ttctgccgga gtggcgataa tgccttcttc cagacagcgc accacttcgt tgaccatcgg
    13741 gatcatcatg cgggcgataa tctcttcttc gctgaaatcg cgcttcggct ggctcacttc
    13801 tgccagcagg tcgtcaacag cggcgtcttc ttctttcttc ggcttacctt tgctgtcttc
    13861 tttataacgc cagaaaccga ggccgttctt ctgaccaaag cggttggcat caaacagcgc
    13921 gtcgatggca tcgcggtaat ctttctgcat ccgctgcggg aagcctgctg ccatgacagc
    13981 ctgggcatga tgcgcggtgt caatgcccac aacgtccagc agatatgccg gacccatagg
    14041 ccagccaaac tgtttttcca tcactttgtc gatcttgcgg aaatccgcgc cgtcgcgcag
    14101 cagctggctg aaacccgcga agtaggggaa cagcacgcgg ttaacgaaga atccggggca
    14161 gtcgttaacc acaatcggcg ttttgcccat cttgctcgcc caggcgacga ctttcgcgat
    14221 ggtttcgtcg gagcttttct cgccgcgaat aatttctacc aacggcatcc ggtgtaccgg
    14281 gttaaaaaag tgcattccgc agaagttttc cggacgttcc agcgcgttgg ccagctcgct
    14341 gataggaatg gttgaagtgt tagacgccag cacggtatcc gggcgtactt tttgctcggt
    14401 ttctgccagc acggcttttt tcactttcgg gttttcgaca accgcttcta ccacaacatc
    14461 cacgcggtca aatccggcgt agtcgagcgt tgggtggatt gtggagatca cgccagccag
    14521 tttcagacca tcgatcttgc cgcgctcaag ctgcttgttc agcagtttcg cggcttcagt
    14581 catgccgagg gttaacgact tgtcgttgat atctttcatg ataaccggca cacctttcca
    14641 cgcagactgg taagcgatgc cgccgcccat aatgcctgcg cccagcacag cggcccgttt
    14701 cggcgtttca acgtctttgg tgagtttctt cgctttgcct tttacatatt gatcgttaag
    14761 gaatatgccg accaatgcgc gagcttcgtt ggtatgcgcc agcgggacaa aacttttgtt
    14821 ttccaggttt agggcttctt cacgaccaaa acgggccgca gcttcaatgg tttttactgc
    14881 ggtgatgggg gccggataat gtttccccgc cgtttgtgcg accatccctt tagcgatggt
    14941 gaagctcatg atggcttcaa tcttgctcag ttttagcggt tccagcttcg gctgacgttt
    15001 cgctttccag tcgaggtcgc cgttaatggc ctggcgtaaa atcgccatcg ccccttcaat
    15061 cagtttttct gctttgacta caccatccac cagaccgatt ttcagcgcct gatccgcgcc
    15121 gacatcttta ccggcggcaa tgatttccag cgcactgtca gcgcccagca tacgtggcat
    15181 acgtacagaa ccgccaaagc ccggcatgat gcccagtttg gtttccggca gaccgatgcg
    15241 cagatccggc gtcgccagac gataatcggt cgccagcacg cattcgcagc caccgcccag
    15301 cgcatagccg ttgacggcag caatggtcgg caccgggaga tcttccaggc gattaaacac
    15361 gctattggca aagtgcagcc actgacttaa ctgttcttca ggaacgagga acagggacaa
    15421 aaattcggtg atatcagcac cgacgataaa ggctgctttg ttcgaacgca gcagcagccc
    15481 ttttagatct gattgctgtt ccagcacgcc gatggcctcg ccgaggctgg cgacggtcgc
    15541 agtgtcgagt ttattaactg aacctggggc atcaaatacc agttcggcaa tgccatcttc
    15601 cagccagtca aggtacaggg tgtcgccttt gtaaagcatg tcagtctcct gaatccgcaa
    15661 ggtgatctgg tcgtaccaga tgcatcgaag tgtgtatttt gtgttaaaaa tatgcaaata
    15721 aaagattaaa gaaatgccga tctgatcacg ctcggcagaa atcacgctct ggatgaacga
    15781 tgtgctaaga tgcggagact taaggtcaaa aaaacagaag ggtaaaaaat ggaatcactg
    15841 gcctcactct ataaaaatca tatagctacc ttacaagaac ggactcgcga tgcgctggcg
    15901 cgcttcaagc tggatgcgtt acttattcac tccggcgaac tgttcaacgt ttttctcgac
    15961 gatcatccct atccgtttaa agtgaatccg caattcaaag cgtgggtgcc ggtaactcag
    16021 gtgccaaact gctggctgtt ggtggatggc gtgaacaagc cgaaactgtg gttttatctg
    16081 ccggttgatt actggcacaa cgtcgaaccg ctgccgacct ccttctggac tgaagatgta
    16141 gaagtgatcg cgctgccgaa agccgatggc attggtagcc tgctgcctgc tgcgcgcggg
    16201 aatatcggtt atatcggtcc ggtgccggag cgtgcgctgc aactgggtat tgaggccagc
    16261 aatatcaacc cgaaaggggt gatcgactac ctgcattact accgctcctt caaaaccgag
    16321 tacgaactgg cctgtatgcg tgaagcgcag aaaatggcgg tcaacggtca tcgcgcggca
    16381 gaagaagcgt tccgttctgg catgagcgag ttcgatatca atattgccta tctgaccgcg
    16441 accggtcatc gtgataccga cgtaccttac agcaacattg tggcgcttaa cgaacacgct
    16501 gcggtgttgc attacaccaa actggaccac caggcaccgg aagagatgcg cagcttcctg
    16561 ctggatgccg gggccgaata taacggctat gcggctgacc tgacccgtac ctggtcggca
    16621 aaaagcgata acgactacgc acagctggtg aaagacgtaa atgatgaaca actggcgctg
    16681 atcgccacca tgaaagcagg cgtcagctat gtggattacc acatccagtt ccatcagcgc
    16741 atcgccaaac tgctgcgtaa acatcaaatc atcaccgata tgagtgaaga agcgatggtc
    16801 gaaaacgatc tcaccggacc gtttatgccg cacggtatcg gtcatccgct gggcctgcag
    16861 gtgcatgacg tcgccggttt tatgcaggat gatagcggta cacacctcgc ggcaccggca
    16921 aaatatccgt acctgcgctg cacccgtatt ctccagccgg gcatggtgtt aaccatcgag
    16981 ccgggtatct acttcatcga atcgctactg gcaccgtggc gtgaagggca gttcagtaaa
    17041 cactttaact ggcagaaaat tgaagcactg aaaccgttcg gcggcattcg tatcgaagac
    17101 aacgtggtga tccacgaaaa caacgtggaa aacatgaccc gggatctgaa actggcgtga
    17161 tggaaagctg gttaattcct gcggcaccag tcacggtcgt tgaagagatc aaaaagagcc
    17221 gcttcataac gatgttggcg cataccgatg gcgttgaggc ggcgaaagcg tttgttgaat
    17281 cggtgcgggc agaacacccc gatgcccgcc accattgcgt ggcgtgggtg gcgggtgcgc
    17341 cggatgattc tcaacagctc ggtttctctg acgacgggga accggcggga acggccggta
    17401 aaccgatgct cgcccaacta atgggcagcg gcgtcgggga aattaccgct gtggtggtgc
    17461 gctactacgg cggcatattg cttggtaccg gtgggttagt gaaagcgtat ggcggcggag
    17521 tgaatcaggc gctgcgccag ctaacgaccc aacgcaagac gccattaacc gaatatactt
    17581 tgcaatgtga atatagtcag ttaaccggca ttgaagcgtt gctggggcag tgtgacggca
    17641 aaattatcaa cagtgattat caggcattcg ttctgctgcg ggtggcgctt ccggcggcga
    17701 aagtggctga attttccgca aaactggcgg attttagccg tggttcattg caattgttag
    17761 cgattgaaga ataatcccca cttcgttttg cagaactaag gaagcggcag agatgcattt
    17821 tcgcgccatt acccgaatcg ttggactact ggtcatctta ttttcaggga ccatgattat
    17881 ccctgggctg gtagcactca tctaccggga tggagcgggc cgcgctttta cccagacctt
    17941 ttttgtcgcc ctcgccattg gctctatgct gtggtggccg aaccgtaaag agaagggcga
    18001 actgaaatcc cgtgaggggt ttctgatagt ggtgctgttc tggaccgtgc tgggtagtgt
    18061 cggtgcgctc ccttttatct tctcggaaag cccgaacctc acgattaccg atgcgttttt
    18121 tgaatctttc tctggcctga ccaccacggg ggccactacg ctggtggggc tggattcgct
    18181 ccctcacgcc atcctatttt atcgccagat gctgcaatgg tttggcggga tggggatcat
    18241 cgtgttggcg gttgcgatac tgcctatcct cggcgtgggt gggatgcagc tctatcgcgc
    18301 agaaatgccc ggcccgctga aagataacaa aatgcgcccg cgaattgcgg aaacggcgaa
    18361 aaccctgtgg ttgatttatg tcttgctgac cgtcgcctgt gcgctggcgt tgtggtttgc
    18421 cggaatggat gcctttgatg ccattggcca tagctttgcg actatcgcta ttggcggctt
    18481 ctcgacacat gatgccagta tcggttattt cgacagcccg actattaaca ctatcattgc
    18541 tatcttcctg ctgatctccg gctgtaacta cggtctgcac ttttcgctgt taagtgggcg
    18601 tagtctgaag gtttattggc gcgatccgga atttcgcatg tttatcggcg tacagtttac
    18661 gctggtggtt atttgtaccc tcgtactgtg gtttcataat gtctacagtt cggcgctgat
    18721 gacaattaac caggcgtttt tccaggtggt gtcgatggcg acaaccgccg ggtttacgac
    18781 tgacagcatt gctcgctggc cgctcttttt accggtactg cttttatgtt cagcttttat
    18841 cggcggttgt gccgggtcaa cgggcggtgg cctgaaagtg atccgcatcc tgctgctgtt
    18901 taagcagggg aaccgtgagc tgaaacgact ggtgcatccg aacgccgtct atagcattaa
    18961 gctggggaat cgcgcactgc cggaacgtat cctcgaagcc gtttggggat ttttctccgc
    19021 ctatgcattg gtgtttattg tcagtatgct ggcgattatc gccacgggcg tggatgactt
    19081 ttctgccttt gcctcagttg ttgcgacatt gaataacctg gggccagggc ttggcgtggt
    19141 tgctgataac tttaccagta tgaacccggt ggctaaatgg atcctgattg ccaacatgct
    19201 gtttggtcgt ctcgaggtct ttacattgct ggtgctcttt accccgactt tctggcgtga
    19261 atgatggagt aatacgtgaa aacattaatt cttttctcaa caagggacgg acaaacgcgc
    19321 gagattgcct cctacctggc ttcggaactg aaagaactgg ggatccaggc ggatgtcgcc
    19381 aatgtgcacc gcattgaaga accacaatgg gaaaactatg accgtgtggt cattggtgct
    19441 tctattcgct atggtcacta ccactctgcg ttccaggagt ttgtcaaaaa gcatgcgacg
    19501 cggctgaatt cgatgccgag cgccttttac tccgtaaacc tggtggcgcg taaaccggag
    19561 aagcgtactc cacagaccaa cagctacgcg cggaagtttc tgatgagttc acaatggcgt
    19621 cccgatcgct gtgccgtcat tgccggggcg ctgcgttatc cacgttaccg ctggtacgac
    19681 cgttttatga tcaagctaat tatgaagatg tcaggcggtg aaacggatac gcgtaaagaa
    19741 gttgtctata ccgattggga gcaggtggcg aatttcgccc gagaaatcgc ccatttaacc
    19801 gacaaatcga cgctgaaata agcataaaga ataaaaaatg cgcggtcgga aaattatttt
    19861 aaatttcctc ttgtcaggcc ggaataactc cctataatgc gccaccactg acacggaaca
    19921 acggcaaaca cgccgccggg tcagcggggt tctcctgaga atctcaacag agaaaagcaa
    19981 aaaaatgctt gactctgtag cgggaaggcg tattatgcac accccgcgcc gctgagaaaa
    20041 agcgaagcgg
//
LOCUS       DACBVI010000080        19973 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.080, whole genome shotgun sequence.
ACCESSION   DACBVI010000080 DACBVI010000000
VERSION     DACBVI010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.080"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..738)
                     /gene="aroD"
                     /locus_tag="IU325_13895"
     CDS             complement(<1..738)
                     /gene="aroD"
                     /locus_tag="IU325_13895"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP5810105.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVDSVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVL"
     gene            complement(769..1635)
                     /gene="ydiB"
                     /locus_tag="IU325_13900"
     CDS             complement(769..1635)
                     /gene="ydiB"
                     /locus_tag="IU325_13900"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HAP5810106.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1647..2912)
                     /locus_tag="IU325_13905"
     CDS             complement(1647..2912)
                     /locus_tag="IU325_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5810107.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYCLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIALDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3139..4353)
                     /locus_tag="IU325_13910"
     CDS             complement(3139..4353)
                     /locus_tag="IU325_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5810108.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTARATT
                     LLMLYTFISFTALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKETR
                     HHSLKENVAPGG"
     gene            complement(4452..4808)
                     /locus_tag="IU325_13915"
     CDS             complement(4452..4808)
                     /locus_tag="IU325_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1870 family protein"
                     /protein_id="HAP5810109.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKYAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4944..5051)
                     /gene="rprA"
                     /locus_tag="IU325_13920"
     ncRNA           complement(4944..5051)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="IU325_13920"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5237..6349)
                     /gene="ydiK"
                     /locus_tag="IU325_13925"
     CDS             complement(5237..6349)
                     /gene="ydiK"
                     /locus_tag="IU325_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HAP5810110.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVGQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGETEKPNK"
     gene            6738..9794
                     /locus_tag="IU325_13930"
     CDS             6738..9794
                     /locus_tag="IU325_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HAP5810111.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDETLIDERVNALCARLDELIASQQAGVIGWQVCSEL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTYDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPVKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALSAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRVAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALDSQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9791..10201
                     /gene="menI"
                     /locus_tag="IU325_13935"
     CDS             9791..10201
                     /gene="menI"
                     /locus_tag="IU325_13935"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HAP5810112.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10301..10489
                     /locus_tag="IU325_13940"
     CDS             10301..10489
                     /locus_tag="IU325_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810113.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11038..11406
                     /gene="sufA"
                     /locus_tag="IU325_13945"
     CDS             11038..11406
                     /gene="sufA"
                     /locus_tag="IU325_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HAP5810114.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            11415..12902
                     /gene="sufB"
                     /locus_tag="IU325_13950"
     CDS             11415..12902
                     /gene="sufB"
                     /locus_tag="IU325_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP5810115.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGVVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12912..13658
                     /gene="sufC"
                     /locus_tag="IU325_13955"
     CDS             12912..13658
                     /gene="sufC"
                     /locus_tag="IU325_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP5810116.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13633..14904
                     /gene="sufD"
                     /locus_tag="IU325_13960"
     CDS             13633..14904
                     /gene="sufD"
                     /locus_tag="IU325_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP5810117.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTSDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAHSVTHIAVKRGQRPVKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14901..16121
                     /gene="sufS"
                     /locus_tag="IU325_13965"
     CDS             14901..16121
                     /gene="sufS"
                     /locus_tag="IU325_13965"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HAP5810118.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGTKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            16134..16550
                     /gene="sufE"
                     /locus_tag="IU325_13970"
     CDS             16134..16550
                     /gene="sufE"
                     /locus_tag="IU325_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HAP5810119.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSS
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16699..17703
                     /gene="ldtE"
                     /locus_tag="IU325_13975"
     CDS             16699..17703
                     /gene="ldtE"
                     /locus_tag="IU325_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HAP5810120.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKGVDQKLVDKALYRRAGYPVAVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17767..18003)
                     /locus_tag="IU325_13980"
     CDS             complement(17767..18003)
                     /locus_tag="IU325_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major outer membrane lipoprotein"
                     /protein_id="HAP5810121.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18314..19726)
                     /locus_tag="IU325_13985"
     CDS             complement(18314..19726)
                     /locus_tag="IU325_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase I"
                     /protein_id="HAP5810122.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5188 a         4817 c         5136 g         4832 t
ORIGIN      
        1 taatactgtg cgcaaatcat ttaccgagat ttgccctggt gcagaggcct tttttaccgc
       61 accaaaagtt gccgccgagc caaatacttc accagccaga cgagaaatta cgccagtttt
      121 tgccattgac atcgtgatga ttggacgatc ggcatactgc tcctgcatct ccagggtcgc
      181 ggcaagcaac gtcagcacat cgctggtact ttgcggcatc agcgcaatct taggaatatc
      241 ggcgtcgaag gactgcattt tgcgcagacg ggcaatgatt tcttcggctt ccggcgtttt
      301 atggaagtca tggttggaca tgacaacttt cacatcatgc gcgtgggcgt aggcgacggt
      361 ttctttaacc tgatcatcgc cggtaaataa ctccagatcg atcatatcaa ccaggccgct
      421 gtcgatggct gcacgattga gtgcaatata agcctcggtg gaaatcgcct gctcgccgcc
      481 ttctttggca ctgcggaagg taaacagcag cggtttttct ggcatggttt cacggagaat
      541 ttttgccgcc gccatgacag aatccacatt ggagaggtcg gcatagtggt caacacgcca
      601 ttccagaata tcaaagtccg cttcacgata ggcgagagct tcagatttca cgctggcgat
      661 atctttcgcc atcagcgaga cgatgatttt aggtgcgccc gtaccaatga cgagatcttt
      721 tacagttacg gttttcattt tttacccttt ctgcacgcgg tcagcctgtc aggcaccgaa
      781 ccccatgacc tgtttaacat attccagagg gaaatctttg ccagtccata acgtgaactg
      841 ttcagcccct tgccacaaca acatgccgta tccatcaatc gttttgcaac cagcttgttg
      901 cgcctgctgc aataacttcg tcatatgcgg gttatacacg cattcagtga ccagaagtcc
      961 cggatgtaac agactgatat cattaaccaa tgattcattc tcaaggggtt tcatacccac
     1021 ttttgtgcca ttggttaaaa tgtcggcgga agccagggct tcagcaaagg cttgctgatc
     1081 ggcgagatcg gtaaccgtga cgacacaatc ggtgttttcg ttaacccgct gcgcgaaggc
     1141 gagggcttta tcgaagaact catcccgacg gttaaagagt ttaatttctt ttaaaccttc
     1201 aattgccccc tgcgcgccaa tcgccgttga ggcacctccg gcccctaaca gcaccatcgt
     1261 tttgcctttg atatcaaaac cgctctcttt aatggcgcga atatggcccg tgccgtcggt
     1321 gttatagcca cgcagatagc catcatcatt aacgatggtg ttgatggcac ccaccagttt
     1381 ggccgctggc gttaattcat caacatattc acacgccagt tgtttgttcg gcattgatac
     1441 accagttccg cgcattttga gggcttttaa tccttcaatt gctccgggaa aactatcgtt
     1501 atccacttca aaagccatat aggtaaatgg caatcccgct ttttctaagg ctttattctg
     1561 catttcaggc gataaactgt ggcggatggg atatgccatc agcccaatca attcgtattt
     1621 tgcggtaaca tccataatta actcctttaa cctctatgct taattgtgtt taaccgggtg
     1681 ctaaatttac gctcgccaaa ccgcacatca ttttcaggaa taatgaaaac gcggtaatag
     1741 cggataaaga caataattgc ggtaatcagg gccagcagcg cgaaagtaaa atcgagagca
     1801 atgatatatt gcaggccgat gttcgacaga taaccggtaa tcagcggaat aacaaaatta
     1861 gccagtccac ccatcatcat atatatactg gtgactttgg ctttgctttt tgggaaaaac
     1921 tctgacatga ccgaaacgcc gagctgtaaa atgccgccag ctgctgagaa accgataaca
     1981 aaggctccgg cattacacac caatggagaa gggtacaggt agataatggc agctgttatt
     2041 gttgccagtg cagagttaaa tacattagcc cagatgggcc ggaccatttt tttcagcagt
     2101 gcggcaaaaa taaagacaca gaccaacgag cccatactgt aataagagat ggtttttaat
     2161 gcctcagctt ctgacatacc agcaaaagcc atcgcatatt tgggcatcca caccacaatc
     2221 acataaaagg tcgagaatgc ggctacaccg aacagtactg atgaaacacc ttccagccag
     2281 actaacggtt tgctgttcat ttgcggtaat tcattagcta cgctggcgtc caccaactgg
     2341 ctggggaatt tgcttttcaa cagcatcagt gtgatcagta caaacagaat acctggaata
     2401 atcagcccat agccgtacca gatgttattg agcaacatat agctcaccag cattgggtag
     2461 aacatttgcc cgaatgacac catcgcttta accagtatga ccgccgaacc agaggctttc
     2521 ggaaagcatt ccatgagcgc ggggtagcca cccgtatcca gcgctgagtt agcgatacct
     2581 acgcacactg ccagacagta ggcgagagtt aaattcgggc aagcgggaat accaaagaag
     2641 aatagcagat acattattac tgccattaat atcaccgccc gacgaccaaa cttatcggag
     2701 atcacaccga agaataaaat actgatcaat cgccccaaac cgataccgga aattaaatag
     2761 gcaatgcccg cgttgtcagt ggaaaacttt tctgccagag atgacatatt ttgggcaagc
     2821 gtaataacac taatgccgtg caggaaatag ctgaagtaaa tacaaagaac agccaggata
     2881 aatggcgtgc tgaaagcctt attttgagac attttcacag ctccttgctg aaagataatt
     2941 tcgttaccgt acgtattgtt ctccgacggt tcacataatt actatactga agatgatttt
     3001 ccctgggtac atggcataaa gtttatgttt ccaggaatat tgcatgtcat agtttgtttt
     3061 ttatcgcgat tttcgttagt tggcgcagaa agagttattt atccgtaaga tcttgcatga
     3121 cctgaaaaga atattgcatt acccacccgg agcgacattt tcctttagcg agtgatgccg
     3181 cgtttctttg cggctgcgta agccgataaa cagtgccagt aaaaagccga tggcagcgat
     3241 ggcggtatcg aaccacataa tatcggcaat acttctttgg gacagatgag ccgtaatcaa
     3301 cggaatagta aaggtcgcaa tactgcccgc actgtaatag atccctgtag ctttaccttt
     3361 agcgtagggg aaacgttcag ccattaatgt caggccaatt tgcacaacac ctccagcaga
     3421 ggtaaaacca atgacaaaag caaatattat caccacataa aatgtgggat gcaggcagac
     3481 ggtaaacaga gcagtaaatg agataaaggt gtacagcatg agtaatgttg ttgctcgagc
     3541 ggtattacga atgagtggag cggtaataaa tacacaaagc agcgaaccca cggtgtagat
     3601 actgagtagt ttgatcgaca tagtgtatga catgcctgca acaaattgtc cgtactgtgc
     3661 cagccactgg ctaaccagat aaaacgttgc cattgagata tagccatata aggtataact
     3721 ggctaaatcg attattgagc agcgatgttc cgtagagctg gtggttttct ttatgacagg
     3781 taagcgacga cccggatggg gtgggaacgt acaacggtat aaaaacagag cgttaataaa
     3841 cataatgcct gcggcaatca taaaggacca accgaaccac agttcagccc acactaacag
     3901 gctaatgatt agcggtaata aaaattgccc gctggaaaca aatgctttaa ttaaaatatt
     3961 ggctgtaccc ggtgagcgtg gaaaagcttc catcaaactg ggataggtgc ctgcatcgag
     4021 aaaactgttt gccattcccg ccagaaagcc aaaaacataa gcgataatga tgttattggt
     4081 gtgcaggatg ccaaaaaaga aggccatata gcaacacatc ccgagcatga taaaagggcg
     4141 gcgaccaaag cgatcggata ataatcctgc aaaaagcaag acacttaatc gaccaatacc
     4201 cagcgatgag ataactattg agacgcctgc ggcattagtc tgccaaagcg tctccagcga
     4261 ggccatattc aggctcatca aaatgacgcc cataccatgc accaggtaat taaaatacaa
     4321 ccccagtgcg gtagggtaat agggattttt cataggttct acttagcaaa ggtgctttaa
     4381 aaagtgaagc gatgttgtgt cagcatccgt tcattattgt tgtaacaacg acaattcctt
     4441 tctttggaaa attaacaaag gcgctccaga tcgtgtgcat ataattcagc ggctaccgat
     4501 tgatagatct tccagtcaat aaaattgcca tcagggtaga cttgttgaaa cgcggttaag
     4561 tcggggaaaa aacgcatggt gttgttgccg atgcggttat ttattttctc tatgatggca
     4621 tgaagatgtt tttttctttg ctgacgcata gccaacagtt gctcgactac acgatcagga
     4681 atggcgtatt tgccattttc ccattgacgc cagctttcac tgtcaccggt ctgggcaatc
     4741 caggtcgcgc attcatcaat agtcatggca aaaatatggc ggagtgcctg gagttcataa
     4801 gcgttcatcg tgtaatggct gcatgaaata aacaataaaa tataaataac attatcagcc
     4861 tgctgacggc ttgaagagag tcacagtatc ttgtgcaaca ttattggaaa gcgtgagggg
     4921 cgaggtagcg aagcggaaaa atgttaaaaa aaagcccatc gtgggagatg ggcaaagact
     4981 acacacagca attcgttgtt tcactcaggg gatttccatg cttataaatc aatatgttga
     5041 tttataaccg tgagctaata gtaggcatac ggactttttg cgtcgatcag attcgtctca
     5101 ttagtttaaa aactgtaaag aatttgcgac aggtactacc agctgaatgg cggtaagcag
     5161 gaaaatatgg ggaagagcaa gcaaatatca attagcggtg gttaacaatc aggcggcaga
     5221 gccgcctgat gagaaattac ttattcggtt tctcggtttc gccaagttct tcgagaattt
     5281 cttccggttg gtccgtcggc ggtgggacct cttccaccca cgcggcaaac agacgccagg
     5341 aaacggctaa aagaaccgga ccaataaata gaccgatcat cccgaaagca atcaaaccac
     5401 caataacacc agagaggatc aggatcagcg gtaaatcggc acccatgcga attaacattg
     5461 ggcggatgac gttatccagc gtgccaacca caccgctcca cactaacaat accgttcccc
     5521 aggtggtatc accggtccag tagagccaga taatcgccgg aatcagtacc ggcaacggtc
     5581 caagctggac aaggcaggag aggatcatta acaccgtgag caacgttgca taaggtacgc
     5641 cggatacggc gaggccgata ccgccaagca ctgcctgtac taacgctgtc accacaacac
     5701 ccagcgccac cgcgcggata gcttgtgccg ctagcagcac ggcggcatct ccacgaacgc
     5761 ctgccagacg ggttgcaaaa tggcgaatgc cttgccccac ttgttcaccg cgccagtaca
     5821 gcagggcgct gaagagaagc atcagcgcac aatgcaccat aaagcgcccg atatgcgccg
     5881 cctgcccaac gaaccaggtg gtggtggtgc caatataagg acggactttt gccatgatcg
     5941 ccgtcccccc catatccagc aagttgtgcc agcctgcata cagcttcgcg ccaatcaccg
     6001 gaatggtatt aagccacgcc agatcgggta acgtcatgtc accgctggaa atggctttaa
     6061 ttagcgggcc gctgccgtcg acgatactgt taaccagcaa agcaataggg atgataaaca
     6121 ccatcactaa taacagcgtc atcaccagaa cggcgagaga gcggcggcca aacatgatct
     6181 tttgcaaacg taacaatacc ggccaggtgg cgataaccac cgtaccggcc catgcaaagc
     6241 cgagaataaa gggttgaaca atccacagac atgccacaat catgatggct aaaaacagca
     6301 ccgaaagcag aatttgtgcg acatccctgg gctgacgaac atttaccata acaacttttc
     6361 accttttttg tgcgtcaaat cgtcgacgcg acgtgaacac gcgaaactca cctgcataat
     6421 gatgagcaat ttcagcggtt tttaacaggc cgattctgcc cttaattctg atgggcgtgt
     6481 tagaaaaaaa tgtggtacaa caactcaggc aacacgcaaa cgatttactc gtcgtatttc
     6541 aagttgcatg tgctgcggct acgttcgttc accccagtca cttactattg taagctcctg
     6601 gagttcactc gcttgccgcc tttctgcaac acgaaatcca tagagtaaac aaacatcacc
     6661 tttcagtgcg ctattgcagc agcctgaaag aatagtgtat cgacctacat cacagaccgc
     6721 aggaaagggt caatataatg attccacaga tttcccaggc acccggcgtc gttcaactgg
     6781 tgcttaattt tttgcaagag ctggagcaac aggggtttac cggcgatacg gcgacaagtt
     6841 atgccgatcg tctgacaatg tcgaccgaca acagtattta ccaacttctc cccgatgcgg
     6901 tggtatttcc gcgttcaacc gcagatgtgg cgctgatcgc ccgtcttgcc gcgcaggaac
     6961 gctattcatc gctgatcttt accccccgcg gcggcggcac cggcactaac ggtcaggcgc
     7021 tcaaccaggg gattattgtt gatatgtccc gccatatgaa ccgcatcatc gaaattaacc
     7081 ctgaagaggg ctgggtgcgc gttgaggccg gggtgataaa agatcaactc aatcagtacc
     7141 tgaaaccgtt cggctacttt tttgcgccgg aactttcgac cagcaaccgg gcaacgctcg
     7201 gtgggatgat caataccgat gcatccggtc agggatcgct ggtctatggc aaaacgtcag
     7261 atcacgtact tggcgtacgc gcggtgttgt tggggggcga tattctcgat acgcaacctt
     7321 tacccgtcga gctggcggaa acgctgggta aatccaatac cacaatcggg cgaatttata
     7381 acacggttta tcaacgttgc cgtcagcaac gccagttaat catcgacaac ttccccaaac
     7441 ttaaccgctt tcttaccggt tacgatctgc gtcatgtctt taacgacgag atgaccgagt
     7501 ttgacctgac gcgcattctg acgggttcag aagggacgct ggcctttatt accgaagcgc
     7561 ggctggatat tacgcccttg cctaaagtgc gccgtctggt gaacgtcaaa tatgactctt
     7621 ttgactccgc gctgcgtaac gcgccgttta tggttgaggc gcgggcgctt tcggtagaga
     7681 cggtggactc aaaagtgctg aatctggcgc gggaagatat tgtctggcat tccgtcagcg
     7741 agttgattac cgatgtgcct gacaaagaaa tgctcgggct gaacattgtg gaatttgctg
     7801 gtgatgatga gacgctgatt gatgagcgag taaatgcact ctgtgcgcgg ctggatgaac
     7861 ttatcgccag tcagcaagca ggtgtgatcg gctggcaggt atgcagtgag ctggcaggtg
     7921 ttgagcgtat ttatgcgatg cgcaaaaaag ctgtcggcct gctcggtaat gccaaaggag
     7981 cagccaagcc aattccgttt gctgaggata cctgcgtacc gccggaacat ctggcagatt
     8041 atattgctga atttcgcgcg ctgctcgaca gccacggctt aagctacggt atgtttggtc
     8101 acgtcgatgc aggtgtcctg cacgtccgtc cggcactgga tatgtgcgat ccgcaacaag
     8161 agattttgat gaagcaaatc tctgatgatg tagtggcgct taccgcgaaa tacggtggtt
     8221 tgttgtgggg cgagcacggc aaaggttttc gcgccgaata cagcccggcg tttttcggtg
     8281 aggaactttt tgcagaactg cgtaaagtga aagcggcatt tgacccgcat aaccgactca
     8341 acccagggaa gatttgcccg ccagaaggtc tcgatgcacc gatgatgaaa gtggacgcgg
     8401 tgaagcgtgg cacatatgat cgacagatcc ccattgcggt acgccagcag tggcgtggtg
     8461 cgatggagtg taacggcaac ggtttatgct tcaactttga tgcccgcagt ccgatgtgcc
     8521 cgtcgatgaa gattacccag aaccggattc attcaccgaa agggcgcgca acgctggtgc
     8581 gtgaatggct gcgtttgttg gcagatcgcg gtgttgaccc agtcaaactg gaacaagagc
     8641 tgcctgaatc aggcgtcagt ttgcggacgt taattgcccg cacgcgcaat agctggcatg
     8701 cgaataaagg cgaatatgac ttctcccacg aagtcaaaga ggcgatgtcg ggctgtctgg
     8761 cctgtaaagc ttgttcgacc cagtgcccaa tcaaaattga cgtgcctgag tttcgttctc
     8821 gctttctgca actctatcac acccgttatt tgcgcccgct gcgcgaccac ctcgtcgcca
     8881 cggtcgagag ctacgcaccg ctgatggcac gcgcgccgaa aacctttaac ttcttcatta
     8941 accagccgct ggtgcgcaaa ctctcggaaa aacatatcgg catggttgat ttgccgctgc
     9001 tgtcggtccc ctcgctacaa caacaaatgg tggggcatcg ctcggcaaac atgacgctgg
     9061 aacagcttga agctctcagt gcagagcaga aagcgcgcac ggtgctggtg gtgcaggacc
     9121 cctttaccag ctattacgat gcgcaagtgg tggcggattt tgtccgtctg gtcgaaaaat
     9181 taggtttcca gccggtgtta ctgccatttt cgccaaatgg caaagcccag catatcaaag
     9241 gctttcttaa tcgttttgcg aagacggcga aaaagacggc ggatttcctc aaccgtgtgg
     9301 cgaagctggg gatgccaatg gtgggagtcg atccggcgct ggtactttgt tatcgcgatg
     9361 aatataaact ggcgctgggc gaggaacgtg gcgagtttaa cgtcttactg gcgaatgaat
     9421 ggctggcaag cgcacttgac tcacagccag tggctacagt cagcggtgaa tcatggtatt
     9481 tctttggtca ctgtaccgaa gttaccgctt tgccgggcgc gccagcacaa tgggccgcga
     9541 tatttgcccg ttttggcgcg aaactggaaa atgtcagcgt gggttgctgc ggcatggcag
     9601 ggacttacgg acatgaagcg aaaaaccata aaaattcgct cgggatctat gagttatcct
     9661 ggcatcaggc gatgcagcga ctgccgcgta atcgctgtct ggcgaccgga tattcctgcc
     9721 gtagccaggt aaaacgggtc gaaggcacgg gggtacgcca tcctgtgcag gctttactgg
     9781 agattattaa atgatatgga aacgaaaaat caccctggaa gcactgaatg ccatgggtga
     9841 aggaaacatg gtggggttgc tggatattcg ctttgaacat attggtgatg acacccttga
     9901 agcgacaatg ccagtagact cgcggacaaa gcagcctttc gggttgctgc atggaggggc
     9961 atccgtggta ctggccgaaa gtatcggttc cgttgccggt tatttatgta ccgaaggtga
    10021 gcaaaaggtg gttggtctgg aaatcaatgc taaccacgtc cgctcggcac gagaagggcg
    10081 ggtgcgcggc gtatgcaaac cgttgcatct cggttcgcgt caccaggtct ggcagattga
    10141 aatcttcgat gagaaagggc gtttgtgctg ttcgtcacga ttgacgaccg ccattttgtg
    10201 acgttgtcac cagaaaagtg tgacgcatat tgcttttggt cattatctgc acagtaaacg
    10261 gtggtatact gtgcaggtct tgtctaaaag cgaggatgct atgtctactc aacttgatcc
    10321 cacccagctg gccattgaat ttttacgccg tgaccaaagc aatctttcac ctgcccaata
    10381 tctcaaacgt ctgaaacaac tagaacttga atttgctgac ctgcttactc tctcttctgc
    10441 tgaattaaaa gaagaaatct actttgcctg gcgtctgggc gtgcattgat cctgacttga
    10501 tgccgcaatt tcgccaaatc gacctggatt aacctccgtc caggttcgat ggccttctta
    10561 atactgctta tctacccgcc tgatattgtt caaaaataat actaatcgca gtttgtgtta
    10621 aaacggcggg ttagctttat gagattattc ttatcgcccc ttcaagagct aagccactga
    10681 gagtgccgga gataagcgcc ggatggggta gaaaccctta agcctgtgtc gcacagactt
    10741 aagggttttc ttatttctga acgctgcact tttttaccta tctcttaaat agtcattttc
    10801 aatacaactt tttctttttc atcacagcct taatgctcct tagataataa tcattatcta
    10861 acaatgagat acctaattct tagcgtgcct gttaacccac acatcagggt ctatgcttat
    10921 taaaagcagg aaaaaagcat gttttacggt aaagcccctg cgtttgctgg gttgaactga
    10981 taatcattat cactaacatg ctgttatacg ctgaaagcga tgaagtgagg taaatcgatg
    11041 gacatgcatt caggaacctt taatccacag gatttcgcct ggcagggctt aacgctgaca
    11101 cccgcagccg ctgtccacat ccgcgagctg gtggcaaagc agccgggtat ggtcggcgtg
    11161 cgcttaggcg tgaagcaaac gggctgcgcg ggctttggct atgtgctcga cagtgttagc
    11221 gagccggaca aagacgatct gctgtttgaa cacgacggcg cgaagctgtt tgtcccgctg
    11281 caagcgatgc cgtttattga tggcacggaa gtcgattttg ttcgtgaagg acttaatcag
    11341 atattcaaat ttcacaaccc taaagcccag aatgaatgtg gctgtggcga aagctttggg
    11401 gtataggcgg tactatgtct cgtaatactg aagcaactga cgatgtcaaa acctggaccg
    11461 gcggcccgct gaattataaa gaaggattct tcacccagtt agccaccgat gagctggcaa
    11521 aggggataaa cgaagaggtg gtgcgcgcaa tttcggcgaa gcgtaatgag ccggagtgga
    11581 tgctggagtt tcgtctcaac gcctatcgcg catggctgga gatggaagaa ccgcactggt
    11641 tgaaagcgca ctacgacaag ctgaattatc aggattacag ctactactca gcaccatcgt
    11701 gcggtaattg tgacgacact tgtgcgtctg aacccggcgt ggtgcagcaa actggcgcga
    11761 acgccttttt aagtaaagag gtggaggcgg cgtttgagca gttgggcgtt cccgtgcggg
    11821 aaggcaaaga ggtggcggtg gacgccattt tcgactctgt ttcggttgcc accacttatc
    11881 gcgaaaaact ggcggagcag ggaattattt tctgttcctt tggcgaggcg atccacgatc
    11941 acccggaact ggtgcgtaaa tatctcggca ccgtggtgcc ggggaatgac aacttctttg
    12001 ccgcacttaa tgcggcggta gcctctgacg gtacgtttat ttatgtgcct aaaggcgtgc
    12061 gctgcccgat ggaactttcc acctattttc gtattaacgc ggaaaaaacc gggcagtttg
    12121 agcgcaccat tctggtggcc gacgaagaca gctacgttag ctacattgaa ggctgttccg
    12181 ctccggtgcg tgacagctat cagttacacg cggcggtggt ggaagtcatc atccataaaa
    12241 acgccgaggt gaaatattcc acggtacaaa actggttccc tggcgataac aacaccggcg
    12301 gtattctcaa cttcgtcacc aagcgtgctt tgtgcgaagg cgaaaacagc aaaatgtcat
    12361 ggacgcaatc agaaaccggg tcagcgatta cgtggaaata tcccagctgt attttgcgcg
    12421 gcgataactc cattggtgag ttttactcag tggcgctgac cagcggtcat cagcaagcgg
    12481 ataccggcac caagatgatc cacatcggta aaaacactaa atcgaccatt atctcgaaag
    12541 ggatctctgc cggacatagt cagaacagtt atcgcggctt agtgaaaatc atgccgacgg
    12601 caaccaatgc gcgcaatttc actcagtgcg actcaatgct gattggcgct aattgtgggg
    12661 cgcatacctt cccgtatgtc gaatgtcgca ataacagcgc acaactggag cacgaggcaa
    12721 caacatcacg tattggtgaa gatcaactgt tttactgcct gcaacgcggg atcagcgaag
    12781 aagacgccat ctcgatgatt gttaacggtt tctgcaaaga cgtgttctca gaactgccgc
    12841 tggaatttgc cgttgaagca caaaaactcc tcgccatcag tcttgaacac agcgtcggat
    12901 aaggaataaa catgttaagt attaaagatt tacacgtcag cgtggaagat aaagctatcc
    12961 tgcgtggatt aagcctcgac gttcgtcccg gcgaagttca cgccattatg gggccaaacg
    13021 gttctggcaa aagtacctta tcggcaacgc ttgccgggcg agaagattat gaggtgacgg
    13081 gcggcacggt tgagttcaaa ggcaaagatt tgcttgcgct gtcgccggaa gatcgtgcag
    13141 gtgaaggcat ctttatggcc ttccagtatc cggtggaaat tcccggcgtc agtaaccagt
    13201 ttttcctgca aacggcactt aatgcggtgc gcagctatcg cggccaggaa acgctcgacc
    13261 gctttgattt tcaggatttg atggaagaga aaatcgctct cctgaagatg ccggaggatt
    13321 tattaacccg ttcggtaaac gttggttttt ccggcggcga gaaaaagcgc aacgatattt
    13381 tgcaaatggc ggtgctggaa ccggagttat gcattcttga tgagtcggac tccgggctgg
    13441 atattgacgc attaaaagtg gttgccgatg gcgtgaactc gctgcgtgac ggcaagcgct
    13501 cattcatcat tgttacgcac taccaacgca ttctcgacta catcaagcct gattacgtcc
    13561 atgtactata tcagggacga attgtgaaat ccggcgattt cacgttggtc aaacaactgg
    13621 aggagcaggg ttatggctgg cttaccgaac agcagtaacg cgctgcaaca gtggcatcac
    13681 ttgtttgaag ctgaagggac aaaacgctca ccgcaagcac agcagcattt acaacaattg
    13741 ctgcgtaccg gactgccgac acgtaaacat gaaaactgga aatatacgcc gctggaaggg
    13801 ctgaccaata gccagtttgt cagcattgcg ggagagatat ccccacagca gcgtgatgcc
    13861 ttagcgttaa cgtcagacgc cgtgcggctg gtgtttgttg atggacgtta cgtgcccgcg
    13921 ctgagcgatg caactgaagg cagcggatat gaagtgagca ttaacgacga ccgtcagggg
    13981 ttacccgacg ctattcaggc ggaagtgttt ctgcatttga cggaaagcct tgcgcatagc
    14041 gtgacgcata tcgccgtgaa gcgcggtcaa cggccggtaa agccattgct gttaatgcat
    14101 atcacccagg gcgtggcagg tgaagaggtg aacactgccc attaccgaca tcatctggat
    14161 ctggcggaag gtgccgaagc aacggtgatc gaacattttg tcagccttaa tgatgctcgt
    14221 cattttaccg gggcacggtt cactatcaat gtcgcagcga acgcccactt gcagcatatc
    14281 aagctggcgt ttgaaaaccc gctcagtcac cactttgccc ataacgattt gttgctggct
    14341 gacgatgcca ccgcatttag ccacagtttc ctgctgggtg gcgcagtgtt acgacacaac
    14401 accagtacgc aactcaatgg cgaaaacagc acgctgcgga tcaatagcct ggcgatgccg
    14461 gtgaaaaacg aggtgtgtga tactcgtacc tggctggaac acaataaagg tttttgtaac
    14521 agccgacagt tgcacaaaac tatcgtcagc gacaaaggcc gcgcggtatt taacggtttg
    14581 atcaacgtcg cgcagcacgc catcaaaacg gatggtcaga tgaccaacaa taatctgctg
    14641 atgggcaaac tggcggaagt ggatacgaaa ccgcagctgg aaatctatgc agatgatgtg
    14701 aaatgcagcc acggcgcgac ggtggggcgc attgatgacg aacagatgtt ttatctgcgc
    14761 tcgcgcggga tcaatcagca agatgcccag cagatgatca tctacgcctt tgctgctgaa
    14821 ctgacggaag cactgcgtga tgaggggctt aaacagcagg tgctggcccg aatcggtcaa
    14881 cggctgccag gaggtgcaag atgacttttt ccgtcgacaa agtgcgggcc gactttccgg
    14941 tgctttcgcg tgaggtaaac ggtttgccgc tggcttatct cgacagcgcc gccagtgcgc
    15001 agaaaccgag ccaggtgatt gatgccgagg ctgagtttta tcgtcatggc tacgcggcgg
    15061 tgcatcgcgg tattcatacc ttaagcgccc aggcgaccga gaaaatggag aacgtgcgca
    15121 agcgggcatc gctgtttatt aatgcccgtt cggcggaaga gctggtgttc gtccgcggca
    15181 cgacggaagg gatcaatctg gtcgccaata gctggggtaa cagcaatgtg cgggcgggcg
    15241 ataacatcat catcagtcag atggagcacc acgctaacat tgttccctgg cagatgcttt
    15301 gcgcacgcgt tggcgcagag ctgcgtgtga tcccgctcaa tcccgacggt acgttgcaac
    15361 tggagacgct acctacgctg tttgatgaga aaactcgcct gctggcaatt actcatgtct
    15421 ccaacgtgct gggaacggaa aatccgctgg cggaaatgat cacgcttgcg catcagcatg
    15481 gcacaaaagt actggtggat ggcgctcagg cggtgatgca tcatccggtg gacgttcagg
    15541 cgctggattg cgatttttac gtgttctccg ggcataaact gtatggcccc actgggattg
    15601 gcattcttta tgtcaaagaa gccttgttgc aggagatgcc gccgtgggaa gggggcggtt
    15661 ctatgatcgc caccgtcagc ctgagtgaag gcactacctg gaccaaagca ccttggcggt
    15721 ttgaagccgg tacacccaat accgggggca tcattggtct tggcgcggcg ctggagtatg
    15781 tgtcggcgct ggggcttaat aatatagccg agtatgaaca gaatctgatg cactacgcgc
    15841 tatcacagct ggaatctgta ccggatctca ctctctatgg tccccagaac agacttggcg
    15901 ttattgcttt taatcttggt aaacaccacg cctatgatgt tggcagtttt ctcgataatt
    15961 acggcattgc tgtgcgtacc ggacatcact gcgctatgcc attaatggcc tattacaacg
    16021 ttcctgccat gtgtcgggcg tcgctggcta tgtataacac ccatgaagaa gtggatcgtc
    16081 tggtgaccgg cctgcaacgt attcaccgtc tgctgggata acagggaggc actatggctt
    16141 tattgccgga taaagaaaaa ttgctgcgta attttttacg ctgcgccaac tgggaagaga
    16201 aatatctcta cattattgag ctgggccagc gtctgccaga attacgcgac gaagacagaa
    16261 gctcacaaaa tagcattcag ggctgccaga gtcaggtgtg gattgtcatg cgccagaatg
    16321 cgcagggaat tattgaattg cagggcgaca gcgatgcggc gattgtgaaa gggcttattg
    16381 cggtcgtctt tattctctac gatcagatga cgccgcagga tattgtcaat ttcgatgtgc
    16441 gtccgtggtt tgaaaaaatg gcgctcactc aacatctcac cccatctcgt tcacaaggtc
    16501 tggaagcgat gattcgcgca attcgcgcca aagccgctgc acttagctaa actaaaggaa
    16561 cagctttcat ccggttggct cacattgccg gatggtcaat gtttacactg acctgcacag
    16621 gagtgttttg gtttcaggtg aacataagcg ccatccgacg tttcagcgtg agtctccggc
    16681 aaatacagga ggtttacaat gaaacgcgcg tctttgctta cactcacgct tatcggcgct
    16741 tttagcgcca tccaggctgc ctgggcggtt gattatccgc tacctccaac cggaagccga
    16801 ctggttgggc aaaatcaaac gtatacggtg caagaagggg ataaaaacct tcaggccatc
    16861 gcccgacgtt ttgatactgc ggcaatgttg atccttgaag ccaataacac tatcgccccg
    16921 gtgccaaaac ctggtacgac gataactatt ccttcgcaac tgttattacc tgatgcgccg
    16981 cgtcagggga ttatcgttaa cctggcagag ctgcgccttt attattatcc gccgggagaa
    17041 aatatcgtgc aggtctatcc gataggtatt ggtttgcagg ggctggaaac gccagtgatg
    17101 gaaacgcgtg tggggcagaa aatccctaac ccaacttgga cgcctacggc gggcattcgt
    17161 cagcgttcgc tggagcgtgg cattaaatta ccgccagtcg ttcctgccgg gccaaataac
    17221 ccgctaggac gttacgcact gcgcctcgcg catggtaatg gcgaatacct cattcatggc
    17281 accagtgcgc cggacagcgt cggtttgcgc gtcagttcgg gctgtattcg catgaatgcg
    17341 ccggatatta aagccttatt ctccagcgtg cggacgggaa cgccggtgaa agtgatcaac
    17401 gaaccggtga aatattccgt ggaaccgaac gggatgcgtt atgttgaagt acatcgacca
    17461 ctatcagcag aagaacagca gaacgttcag acaatgccat acacactgcc agcaggcttt
    17521 acgcaattta aagacaataa gggtgtagat cagaaattag tcgataaagc gttgtatcgt
    17581 cgggcagggt atccggttgc ggtgagcagt ggagcaactc ccgcagccag caatgcgcct
    17641 tcagtagagt cagcgcagaa tggtgaacca gagcaaggga atatgttacg cgcgacgcag
    17701 tagcggtaaa tggcaggcaa aaaaaatggc gcacaatgtg cgccattttt cacttcacag
    17761 gtactattac ttgcggtatt tagtagccat gttgtccaga cgctggttag cacgagctgc
    17821 gtcatcttta gcagcctgaa cgtcggaacg cattgcgttc acgtcgttgc tcagctggtc
    17881 aactttagcg ttcagagtct gaacgtcaga agacagctga tcgattttag cgttgctgga
    17941 gcaacctgcc agcagagtag aacccaggat taccgcgccc agtaccagtt tagtagcttt
    18001 cattattaat accctctaga ttgagttaat ctccatgtag cgttacaagt attacacaaa
    18061 cttttttatg ttgagaatat ttttttgatg ggaaggcact tatttttgat cgttcgctca
    18121 aagaagcatc gaacgctggg ttttagataa aaaaattgag agaaaacagg atgcttccat
    18181 cggattcatc ttagataaag tgagattata tagtgaaatc cgatttgatt ttttattgct
    18241 tctggttatc gattaaataa aaaaagcgcc catcagggcg cttcgatata caaattaatt
    18301 cacaaaagca atattacagg acgtgaacag atgcggtgtt agtagtgccg ctcggtacca
    18361 gtgcaccaga aaccataact acgacgtcac ctttgtgtgc cagaccgctc tgcagagcca
    18421 gttctttacc cagacggtag aaatcatcag tagaagtgat ctctttaacc agctgcggca
    18481 caacgccttt gctcagtacc aactgatgag ccgttttttc gttggtggtc agtgccagga
    18541 tggtggcatc cgggaagtat ttacgtactg cgcgagcaga tttaccgccc tgggtagcaa
    18601 ccacgatcag cggggcatcc agtttttcag cagtttcaac ggcaccacgg cataccgctt
    18661 cggtaatgcg cagtttacgg ttgtcattgt tgaactcgag acggctgttc atcacgcggt
    18721 cggtacgttc gcagatggtc gccatgatag aaaccgcttc cagcgggtat ttaccttttg
    18781 cggattcacc agacagcatc actgcgtcag taccgtcgag gatggcgttt gcaacgtcac
    18841 cggcttctgc gcgagtcggg cgtgggtttt tgatcatgga atccagcatc tgggtcgcag
    18901 tgataacgac tttacgtgca cggatacatt tttcgatcat catcttctgg gcgaagataa
    18961 cttcttctac cgggatttct acacccaggt cgccacgcgc aaccatgatg ccgtcagagg
    19021 cttcgaggat ttcgtcgaag ttgttgaggc cttcctggtt ttcgattttg gagatgatgt
    19081 ggatgttttc gccgccgtgc gctttcaggt gctcacggat ttcgataacg tcagaacgct
    19141 tacgaataaa ggaagcagcg acaaagtcta cgccttgttc gcaaccaaag atcaggtcct
    19201 gtttgtcttt ttcagccagt gctggcaggg caatggaaac gccaggcagg ttcacacctt
    19261 tgttttcacc caggtcgccg ttgttcagca ctttacagat aactttgtta ccttcaatgg
    19321 cagtaacttc cataccgatc agaccatcgt caaccagtac ggtgttgcca acagacaggt
    19381 cagtagtgaa accttcatac gttaccgcaa ccatttcgct gttgccgata acagatttat
    19441 cagtggtgaa agtaaaggtc tgacccgctt tcagagaaac gtcgttaccg ccttccagtt
    19501 tcatggtgcg gatttccgga cctttggtat caagcaggat agcggcggtt ttaccagttt
    19561 tgctcatcac gttgcgcaga ttctgaatgc gctgaccgtg ttctgcatag tcaccatgag
    19621 agaagttcag acgcataacg ttcatgccag cgtccagcat tttagctaac atctcttcag
    19681 attcggtttt cggtccgatg gtgcaaacaa ttttggtctt tttcatgaca gtcttagtct
    19741 ttaagttgag aaggatggga gaaacttgct ttctgggcgc actgccggaa agcgaatccg
    19801 gtattacgaa agtggtggtg cctcgctcta aggataggtg acatcgaaaa agcgtgcaga
    19861 ggaatgtgtg cttgtgtctc agcccaacgt gagtgcctgt ttttgagtcg ttgagttcta
    19921 cagtccaaag tgctgaaacc attcaagagt caattggcgc gcattatacg ctg
//
LOCUS       DACBVI010000081        19726 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.081, whole genome shotgun sequence.
ACCESSION   DACBVI010000081 DACBVI010000000
VERSION     DACBVI010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.081"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            1..657
                     /locus_tag="IU325_13990"
                     /pseudo
     CDS             1..657
                     /locus_tag="IU325_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(677..742)
                     /locus_tag="IU325_13995"
                     /pseudo
     CDS             complement(677..742)
                     /locus_tag="IU325_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
     gene            975..1079
                     /locus_tag="IU325_14000"
                     /pseudo
     CDS             975..1079
                     /locus_tag="IU325_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1417..1674
                     /locus_tag="IU325_14005"
     CDS             1417..1674
                     /locus_tag="IU325_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HAP5810123.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
     gene            complement(1837..2046)
                     /locus_tag="IU325_14010"
     CDS             complement(1837..2046)
                     /locus_tag="IU325_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810124.1"
                     /translation="MLPSETMIWQPEFTDKTLSRKPGAVHLAACPLLLDKIITIHRVK
                     QVVTASLKLRDYDGQVAEAMPMIPK"
     gene            complement(2012..2143)
                     /locus_tag="IU325_14015"
                     /pseudo
     CDS             complement(2012..2143)
                     /locus_tag="IU325_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2425..3783
                     /locus_tag="IU325_14020"
     CDS             2425..3783
                     /locus_tag="IU325_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HAP5810125.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(4022..5407)
                     /locus_tag="IU325_14025"
     CDS             complement(4022..5407)
                     /locus_tag="IU325_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP5810126.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGENTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVPGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(5457..5804)
                     /gene="tnpB"
                     /locus_tag="IU325_14030"
     CDS             complement(5457..5804)
                     /gene="tnpB"
                     /locus_tag="IU325_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP5810127.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSMTML"
     gene            complement(5801..6181)
                     /locus_tag="IU325_14035"
     CDS             complement(5801..6181)
                     /locus_tag="IU325_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAP5810128.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            complement(6536..6970)
                     /locus_tag="IU325_14040"
     CDS             complement(6536..6970)
                     /locus_tag="IU325_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810129.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARVDNPLQRL"
     gene            complement(6958..7359)
                     /locus_tag="IU325_14045"
     CDS             complement(6958..7359)
                     /locus_tag="IU325_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810130.1"
                     /translation="MYAKSFIALDSNGRLTGARTAQAAPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(7619..8188)
                     /locus_tag="IU325_14050"
     CDS             complement(7619..8188)
                     /locus_tag="IU325_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HAP5810131.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGEFKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCYATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            complement(9051..9227)
                     /locus_tag="IU325_14055"
     CDS             complement(9051..9227)
                     /locus_tag="IU325_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HAP5810132.1"
                     /translation="MAARNTSGDAGKVNSHTRYKLTLAELEAFNSAVDNRLAELTMNK
                     LYDRAPASVWKYVT"
     gene            9935..10141
                     /locus_tag="IU325_14060"
     CDS             9935..10141
                     /locus_tag="IU325_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP5810133.1"
                     /translation="MATPVSLMDDQMVDMAFLTQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            10236..10838
                     /locus_tag="IU325_14065"
     CDS             10236..10838
                     /locus_tag="IU325_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001760115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810134.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            12041..12958
                     /locus_tag="IU325_14070"
     CDS             12041..12958
                     /locus_tag="IU325_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810135.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKEKNIALDNLASFPGSADIYHA
                     IHLSFDIWVTHPSASPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRARRLSTGEMLHDLIVEEYKRYRHSR"
     gene            13042..13914
                     /locus_tag="IU325_14075"
     CDS             13042..13914
                     /locus_tag="IU325_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HAP5810136.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNTLFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRAQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            14286..17132
                     /gene="ag43"
                     /locus_tag="IU325_14080"
     CDS             14286..17132
                     /gene="ag43"
                     /locus_tag="IU325_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HAP5810137.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVLGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQAVKSGAMATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATHVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNAGGQASNTTVGHRGTLMLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPDAALSRAVAKGNAPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYTS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRVGTVRDDAGSLGGYLNL
                     THTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     QYTWQGLSLDDGQDNAGYVKFGHGSTQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSAK
                     HRVRELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            17203..17361
                     /locus_tag="IU325_14085"
     CDS             17203..17361
                     /locus_tag="IU325_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HAP5810138.1"
                     /translation="MPGCTSRLLPEGPFSREQAVAVKTAYRNVFTEDDQGTYSRLVIR
                     NAEGQLRW"
     gene            17516..18334
                     /locus_tag="IU325_14090"
     CDS             17516..18334
                     /locus_tag="IU325_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HAP5810139.1"
                     /translation="MRLASRFGYTNQIRRDRPLTHEELMHYVPGIFGEDRHTSRSKRY
                     AYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSARGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            18673..19146
                     /locus_tag="IU325_14095"
     CDS             18673..19146
                     /locus_tag="IU325_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAP5810140.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCENYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            19162..19638
                     /gene="radC"
                     /locus_tag="IU325_14100"
     CDS             19162..19638
                     /gene="radC"
                     /locus_tag="IU325_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAP5810141.1"
                     /translation="MQQLSFLPGNMTPGERSLIQRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGNQVFSFAEHGLL"
BASE COUNT         4764 a         5151 c         5112 g         4699 t
ORIGIN      
        1 tgaaggcgtg gtgcgtaacg gcaaaagcac tgccggacat cagcgctatc tctgctctca
       61 ttgccgtaaa acatggcaac tacagttcac ttacaccgcc tctcagcccg gtacgcacca
      121 gaaaatcatt gatatggcca tgaatggcgt cggatgtcgc gccagtgcac gcattatggg
      181 cgttggcctc aacacggttt tacgtcactt aaaaaactca ggccgtagtc ggtaacctcg
      241 cgcatacaac cgggcagtga tgtgattgtc tgcgctgaaa tggacgaaca gtggggctac
      301 gtcggtgcta aatcacgtca gcgctggctg ttttacgcgt atgacaggat acggaggacg
      361 gttgtggcgc acgtcttcgg tgaacgcact ctggccacac tggagcgtct tctgagcctg
      421 ctgtcggtct ttgaggtcgt ggtatagatg acggatggct ggccgctgta tgaatcacgc
      481 ctgaagggaa agctgcacgt tatcagcaag cgttacactc agcgcattga gcgacataat
      541 ctgaatctga gacaacatct ggcaaggctg ggacggaagt tactgtcgtt ctcaaaatcg
      601 gtggagttgc atgacaaggt catcgggcat tatctgaaca taaaacacta tcagtaagtt
      661 ggagtcatta cctcctcccg aactcaaagc tatcatacag ataattactg gttaacccta
      721 tatagggcat tttaaagtgt tgtgatctta cccagcaata gtggacacgc ggctaagtga
      781 gtaaactctc agtcagaggt gactcacaag acaaaacagt ttcaaccagt aaaaaaacgt
      841 aaacaccatt cgcatgaatt tcgtagttaa gatctgaagc ttgcagaatg catcggtgtt
      901 actgccggag tccgtgaact tagtctgcat gaatcaccac agctttacat ctgtcgcagt
      961 aaacagcaaa atcagcaaac ttacttaatt atatcgagtg cgattacaat cgctggcgat
     1021 ggtacagttg gtgtggcggt ctcagtccgg aacaatttga aaatcagaat ctcacttaaa
     1081 acggcatcca ttttacaaaa gatggccgtc tgtgtattta taatgaatac cttctacatg
     1141 tttagagtgc ataatccacg acaaggcgat atcctaaaaa ttagctagca ggcaactttg
     1201 atggcataga taatgttttt agtaaaaaag agttgactga atataataca gcctcttcct
     1261 acataagaag gaatgtgctc acaaaaaaac cattagtctg cttcctgttc ttacaataat
     1321 cagtattatg tattttattt atatagcaaa atatgcttgt tacacctgtc ataattaggc
     1381 atggtaaaat tattgactga accctggggg agaaagatgc acagtgatta ttataatatg
     1441 gtttttggtg aaaaattggc caatatttta tatgaagcta actctcaatt tttttacgag
     1501 agaaatgtca tagaagaggc agtcaacgct ttattctgtg agcgtgaaat aataaacaac
     1561 aagaacataa taaagaagct gatgttcttt ttgtcagatg ttaatcacac caagaaagat
     1621 gttgttcaga gcgcacttaa tattatcatt gacattacat caggtgatat ttgagatttt
     1681 acataaaaga taatctctca aggagtctta tctgaaaacg aaatatatag tccctctcgt
     1741 tttgtaatat cgattggctt tgttgaatcg gttacgtacc ccggagccgg tcatattttc
     1801 aggtaataca cacgctttca gacatttcgg ctttcgtcat tttggtatca tgggcatagc
     1861 ttctgctacc tgaccatcat aatcacgcag cttcagtgaa gcagtgacca cctgtttaac
     1921 ccggtgtatc gttatgatct tatccagcaa taatggacac gcggctaagt gaaccgcccc
     1981 gggtttcctg gagagtgttt tatctgtgaa ctcaggctgc cagatcatcg tttccgatgg
     2041 aagcataata agctttttct gcttctgccg gtgggatatg gccctgcctt tccagcaatc
     2101 gtcgattgtt ataccagtcc acccacgtga gtgtggtcag ctcatcatac tgacacgtgc
     2161 attaccccta ccgcgataat gcaaccgtac aatatagggc gtttcctaaa cgcagccctc
     2221 atcactccaa tccaacaaga cacagcattt ttgatcacga agaaagacaa ataatttccg
     2281 taaatgtttc cgttgttaca aataacaaca gtgttttaca tttctttggc ctcaggcccg
     2341 cgcatgttgc cattcgatgt cattttcctg actcgtttct tctttatggt gtagtgtgat
     2401 cttcacttta tggatgctaa caatatgaaa agaaaaatta ttcccgtgct aatcggttgt
     2461 acgctctctt tctctgccct ggcggcacaa cctacggctg agcgctatgt cgtcagtttt
     2521 ccagaaggca cgcacgtgaa ctacgccggc gcatttgcca gtgcattccc gaacggcctt
     2581 ccggttggga taggttctgg cctgctgttc accggtaaac agggcgatgc cctgacattc
     2641 gcaaccatta ccgatcgcgg tccgaacgcg gattcaccga aagaggggaa aaacgaaact
     2701 aaaattttcg ttacgccaga tttcgcgccg ctgctgatga cgattcgtgt acaaaacggc
     2761 aaagcggagg ccatcgatcc ccgtcctctg catgacgata aaggcgcgat caacggtctg
     2821 ccgctggcaa gcgacgtgat tggctccacc aatgaagtgg ccttcagcga cacgttacat
     2881 agactgaagg gggataatcg cgggctggat acggaaggga tcactccgga tggtaaagga
     2941 ggttactggc tgtgcgatga gtatggcccg ttcctgatta acatcgacag taaagggaaa
     3001 atcctggcaa tccacggtcc gcaggcggcg gaaggggaaa aagcgattgc gggcggtttg
     3061 ccaaatatcc tcaaatggcg tcaggcgaac cgcggctttg aaggcctcac ccgtatgccg
     3121 gacgggcgta ttattgtcgc cgtgcaaagt acgctggata tcgacgctaa gagcaagaaa
     3181 aaggcgctgt ttacgcgtct ggtgagcttc gatccggcaa gcggaaaaac cgcgatgtac
     3241 ggttatccta tcgacagcgc ggcctacagc aaaaacagcg acgctaaaat cggagacatc
     3301 gtggcgctcg ataaccagca catcctgctg atcgaacagg gaagagataa aaacaacaga
     3361 atgcgtaacc tgatttacga ggtcgatctc aataaggcga gcgacctgtc aggcttcgac
     3421 aagccgggtg agtatccgga gtttgatgat gagaaaaccc tgtcacagcg cggcattacc
     3481 cttgcgcaga aaacgcaggt tgttgatctg cgctccttgg gctggcagca ggagaaggct
     3541 gaagggttgg cgctgatcga cagcaaaacg ctggcggtgg cgaacgataa cgactttggc
     3601 gtgaaggtgg cgatgcaaca tccggtcgag ggcaagacat tcaaggatta tcgggtgaac
     3661 gcggaaggaa agctgacgct tgacgacaag caggttgaaa cgaccctcag agtgaagcca
     3721 ctggagaaac cggagtctga cagtgaactg tggattgtga cgctgccgga agcgttgaaa
     3781 taagtttata aggtacggac agcagtatcg cttagttttt caaattcatg tatgtgaaca
     3841 atattggggt tccggcgtag acccccaatg aaagcgtcac cacgtgaaaa catgcctgca
     3901 cggtttacac gtcatctcat ggtggcagcc ggttaacttt cctgctacac cagtggaaaa
     3961 aggccataag cagtccgaac aaaagtaagc gtacagcgag ggccgtattg acggggatgt
     4021 gttattcagc tggcagtgct atgcgccacg gaagcagttc gctgacccgg ttgaccggcc
     4081 agtctgctat gacgccaggc acatggcgaa ggtagctttc tggatccacg tcattcagtt
     4141 tgcacgtccc gatcaggctg tacagtagcg ctccccgctc accaccatgg ccagagccga
     4201 agaacagggt cttcatcttt tcacgcaacc agctttccag ggatttcaac agcggtttcg
     4261 tttttcgctg acgttcagca agccgctgct ctgccggcat tccccttata tccgcctcta
     4321 tggcgtacaa ctgaccgatc tgctctaggg cttcttccgt cagtgctgac gggatgcgga
     4381 cgtgcacatc gtggatcttt cggcgggcat gagcccagca ggcagcttcc gttatcccac
     4441 cattgcgata cagctcgttg aacccggcgt acgcatccgc ttgcagcaca ccgctgaagc
     4501 aagcaagatg agtctgcgga tggatgcctt ttctgtccgg gctgtaagcg aaccacactg
     4561 caggtgccaa cgctgacccg gcattgcggt catcacgaac atacgcccac aaccgcccgg
     4621 tcttcgtctt cttattaccc ggcagcagta cctggaccgg ggtatcatcg gcatggagtt
     4681 tgccgtcagt catgacatag ccatgaagcg cctcttccag cggagacagc agccggcagc
     4741 atgcatccac ccagcccgac agcagtgaac ggctcagctc cacaccttgc cggccgtata
     4801 tttctgactg gcaatacagc ggggtgtgct ctgcatactt cgaggtcagc acgcgggcca
     4861 gcagccccgg tccggcgata ccccgctcga tgggccgcga aggtgcaggt gcctgcacga
     4921 tggcatcgca ctgagtacag gcatgttttt cccgtaccgt ccggataacc cggaaggcgc
     4981 tacgcatcaa ctccagctgt tcggcggtat tctcgcccag atagctcagt gaaccgccgc
     5041 agttcgggca gcacggcgcc gcaggcaaca gtcgcttttc gtcacggggt agtgattcag
     5101 ggaacggctt acgggtgcgg gtctgacgca acggacgctg tactgccggg tcatacaccc
     5161 taccagtcag cgtatcgctc tctttctgaa gccggttcag atcggcttcc atttgtgcga
     5221 tacggcggga gactttttcg gaacgactgc cgaagttcat ccggcggagt ttatccagct
     5281 gcgcctgcag atggtctatt tcgcgctccc ggttgctcag cttttcctgc agggcgtgga
     5341 tcagcgcttc ctgttcggcc aggcgctgtt tcagcaggaa gatgtcgtca gaagagatgt
     5401 cgttcataag cccgtatttt accgggctta ttctgtgaca accaggataa agagatttac
     5461 agcatggtca tggaggtcag cagccgcttg ggctgtcgcc agtcgatacc ttccagcagc
     5521 atcgccagct gcgcctgtgt aaggaacact ttgccatcac gggctgacgg ccaggcgaag
     5581 cgcccacgct ccagccgttt ggtcaggagg cacagtccgt caccggtgga ccacagcagt
     5641 ttaacctgac tgccgctgcg gccccggaaa atgaaaacat ggccggacat gggatcgtct
     5701 ttcagcgccg tctgtacttt cgcagccagg ccgttgaagc catttctcat atcggtgata
     5761 ccggcaacca gccaaatttt ggtcccggaa ggtaacggga tcatcgcttc agttcctgta
     5821 tcagcagagt caggagcttt tcgctgacat tgccattgaa gcggagcgtc ccgtgccgga
     5881 acgttacctc acagctgata ctgagggttt ccgggtcctc tgcgagcgat tctggctgtt
     5941 cggcagctgc atcgagagtc acaggaagta gctgggggct ctctgaagaa ggtaatagca
     6001 gctttccctc gcgccattgt tgtcgccatt tgaacaacag attggcgtta atgccatttt
     6061 caagagcaag ttttgagatg gatatcccgg gttcacagga ggcagcaacg agctgctgtt
     6121 taaattcggg aggataatta gggcagcctt ttcgcctgcc gggagtcaca tttttctgca
     6181 tatctgacac tttggttccc actacttatt tggtggacac cactttgtct aattcgtcag
     6241 attctgacca gacggttcag gctgtacgct taccactatt ttatccctgt tgaatttggt
     6301 taagccggtg cagacgcgcc gggaaacgca cacctgaacc ggcacaataa aatacaatac
     6361 aaccccctgt tactggagat acgaatggaa tgacgaggca gcctgtcagt cacagggggc
     6421 tgttggcata aaatcaacgg aaaggccacc aacaccggaa ttaagctcca cagtccccgc
     6481 actggcaatc cattcccggg aaaggcatta ccgcaaactg ctccggaggc aaccattaca
     6541 gcctctgtaa cggattatca accctggcat cttccgacgg cacctcctgc cgcccccacg
     6601 ccctgctgta taccccggta ccgcagcgtg ggcagcactt ttccccctca taatcttcgt
     6661 cacacatcac acaaaaccac tttctcacct tcaccgtata atccggaacc gtcatgccct
     6721 gcagatgcaa acgacgggcc tctttctctg ccgggtcaag ccaggtacat cgcagcagga
     6781 ttttttcagc gacagtcatc tggtcatgtt caaaccacgg tggttgtccg tcattgcccc
     6841 gctgaagtgt taacgggcat ccacaggaca cacagcacaa aacgtcctgt cggttgtgct
     6901 tcgcagcttc tttcgccgta acaaaatggc ctttgctgtc attcgccata taacagttca
     6961 gaattcgcat atttcctcct gagttgtccg gggaatactg aatcctccgg tctggcagtg
     7021 tgtgcagtac tgctccccat aataatcgtg gtgacattgt ctgcagtgcc agctggcttt
     7081 acgcaccacg ggtaaggcat ccggtacgaa ttgctgcaga cgtttaatca actgtatttc
     7141 tctgcgctcc ggcctgacat aagggcactg ttgaccgtgc tctgtcagcc tgtcgtcagt
     7201 gtgttcaaac cagggaagtt cagtgtcgta ttgcggatgg tatctgagtg cactgccaca
     7261 caagtggcag gtgtagtgag cataaggtgc ggcctgtgca gtacgggcgc ccgtcagacg
     7321 tccgttgcta tcaagagcga taaaggattt tgcgtacata gtcatattcc tcagccgcca
     7381 gacgacacgc aggcaacagc cgtcccggta cgggcagcgt ggtcagggtg tgaatggcgg
     7441 ttcagttaaa atctggactg gagtttgggt aaagcaggct ggttgctgtt acagcactgt
     7501 ggttgtcaca caatggaaca ctgacgtaaa aaacagtcag tggacgagtg gatacatcgt
     7561 tgccgccaga caacaaccca tacttttggt ttgccggata ttggcgcgcc agtcgtgctc
     7621 aactccggct acagccaaaa ttgcgctcac cggtgcagtg agctttggta tgctccttcg
     7681 ccagatagtc agcacgttcc agcacttgct gaaagccggt gtcatcatta cgcgccagcc
     7741 acaccgccgg ccaggccgga aaatgcacca gcgtggcata gcacccgaca tccactccca
     7801 gggcactgca ccacgcctgt ttaatcatcc cggccagaga ctccggagcg cggtaatcac
     7861 cggcacgaca ccaggtatcc cggttgacca gcagcatcag gtgatagtgt tttttacctt
     7921 tgaactcccc aaactcccgg gcccagacgt aatgcagggt tgtgggatgc acgcgtttac
     7981 cttcccgatg cttacgtttc tggtaagcgt cgattcgggc tttcagggca ttgatgaagc
     8041 gggatatcac agctgcgtcc gttgctgccg gtacatccgg gagacgcagg tcaacccgca
     8101 atgccgtcag gcggggatga acattcagtg cgtgccgcac cgtctcacga atacgttgct
     8161 gccagaaggg gttgtatttg taggtcatgg ttaaatctcc gtatggttca tacggaataa
     8221 ccacgtcgta aaaaatgcgc agagtccctg aggtgacctc cggcaggaca ctgtctcagg
     8281 cactctgtga taacccgaac ggtcgtctcc ggactggcgg ttgaattttc agcgtcgttt
     8341 tttaatgtaa aaaaccggtt acgggtaagg ctgtgaggag ggagttatgg tgatacgcaa
     8401 aaagaagtgc agggactgtg gaaacgcgat tactaataat acagagcctg tttaagattc
     8461 tgtgtaaatg ccttgtctca gacgtgaccg tccaggcggt caccgaactc gataataaag
     8521 cggctcattg ccattcgcca gtccttcaac ggcatcgtcc agtgtgttgc ccgtattgtg
     8581 gttctgtcga ccccttcggc tactaccgga atacagaccg tatcgttacc atactgctgg
     8641 cgctgattat tgtggtcctg ctgactactg tcagcgtcag tgtgtgtata ctgtgtagct
     8701 ggtgagataa cggttcatgt atcccctgag ctgacagtgt accgtgtata ttaagcgtaa
     8761 ccgacaggtt ctccttcata ccatattaaa ttttccaggt attgccgctg ccttatttcg
     8821 tcccatacag gaaaactcat ctgggtttct gacaaatacg cgaaactcac agcccctcaa
     8881 tatcaaccac acgttgtaag ccggtaatac ctgaaaatca gcctattacc gcccacgcct
     8941 ccggcaccat tcccttttga tttcgaattc tttgttgtcg ctataccgct taatgttccc
     9001 ggtcctccgg tgcagataac accggagtca tcgtagcgct gcttcaatat tcaggtgaca
     9061 tatttccaga cggaagccgg cgcgcgatcg taaagtttgt tcattgtcag ttctgccagc
     9121 cggttgtcaa cggcagagtt aaaggcttcc agctccgcaa gggtaagttt ataacgcgtg
     9181 tgggaattca cttttcccgc gtctcctgac gtattacggg ctgccactta cctggtgtcc
     9241 cgcaatacag ccttagtgtt ccgttatcgg ggatccggac gcaccgcaac aaccggggaa
     9301 gtatttctgg tttagagcat tgtcaagagg gatttctcag cccctcactc accggctcag
     9361 tctcagggca ggagcattaa tacgctcagt tcatgtctta tacaggaatg ggcacagggg
     9421 gagctggcgt gtcactgctg attttacgca tcagcgttca tgtatctgca ctctgataca
     9481 tgaacgattc acagcgctct gttaccggag agatttactc tcttagctta ctctggcaaa
     9541 tcctttacgt tactctctga tgacttctta tatttataat accaatcaga cagccttatc
     9601 ccatcggtga tatcagtctc ctcctcgcca ggaacgggct gactcactgt aaccacctcc
     9661 gaaaacaact ctgcaattat cagaacgcct gcttctctcc ctgtcctcac gaaaactatc
     9721 ccctctttat cgcgcgtgcg tgcggaagca tcttttcgca acaaccaccc ggtataccgg
     9781 tacggctcag ccaccgcaac ctccccgttt atctcctggc ggtcacattc ccgattattt
     9841 tttacgtttc tccccggttg ttatgccggt gaaggtggcg cgctgttttc atgaccacac
     9901 cggctggttt aacaacatcc ggaggaacat tctcatggct accccagttt cactgatgga
     9961 tgaccagatg gtcgacatgg catttctcac tcaactgacc ggcctgacag ataagtggtt
    10021 ttacaggctc atcagggatg gggcctttcc ggctcccatc aagctgggcc gcagctcccg
    10081 ctggctgaaa agtgaagtgg aagcctggct acaggcgcgt attgcacagc cccgcccgta
    10141 atttctgacc gttatccgtt cacccgcagc agacgtaatc cccggcctgc cggcggcatt
    10201 ctgctgcctg ttatatcccc gtgaggaata tgaaaatgaa acaacagtat cagacccgct
    10261 acgaatggct ccacgaaagc taccagaaat ggctgaccgg cttcacccgg cacgccgtat
    10321 cctggggcgt gtgtcatccg aatatctact acttccataa tctgacgccc ggttgggtgt
    10381 cattcaatgg tgaaaaaccg gagattgcca tcgtacccca gagcctgcac cggctgattt
    10441 atggccctga caaacgggcc acgccgcccc tggatgatga tctgatagtg aatttatgca
    10501 ccagtgaaca tctgctggtt catcatccga tgctggaagg cattctcctg tctgaatgtg
    10561 aacgtctgag gcagcgttct ctggcaaata aactgattag cctcttccgt cagtttggtg
    10621 gcacggagtt gcgcctcaaa ctggtctggc tttgctggct tgatttaatg accggaaaca
    10681 gccttgaaga ctggaaagag aacctgaaac ggaaatcaga aaaagagctg gaggaatgga
    10741 tcatcaaccg tcagaggcag agcgcagcac tgacggatct gatggatcag tacgtgctcc
    10801 tggcataccg cacaacggtt gacgatagcc gcaactgatg ccggttgctt ccggaaggtg
    10861 tgacaaaata cggaaatgat gatgctcccg gccctgcatg ctcacctgca gagcgtgttc
    10921 gtcgtcatca tactgagcca tgacgggcgt cttaagggca tcctgtcatt gctgagcagg
    10981 caacaccacc agaggagcca cggacaggga tgttctttac agcccgatac acgccaggtt
    11041 ctcagcagac tgaatgtgca ggtgcggtta ttcaccagaa atggcatgct gcggtacata
    11101 cccatcctgg tgacaatatg tacgtggcga cagcgatccc ggcactgtgg aagagactca
    11161 ctcagcctgc aggatattct ttccggcatc cgggaatgtc cgcctgaatg caaacaggat
    11221 gatatttgaa tccgtaatac tgttctttca tcataaataa tttatgcaga tacagttaac
    11281 cgttacagta gcaggtttgt tttaccggga aataatatcc gtcagactga tgccggaaca
    11341 tatctgaaac acgttatgat gttttgttat cagaaatgtt gtatattctt atccgtggcg
    11401 acagtttaat gccctctggt atcaccttgg ataatgcata tcctgcgatg attcgttata
    11461 tttacgggac acgcctcttt tcattgtgct ccttttcaga cttcattttc cgtaagtgtt
    11521 ccccggcata aaggaggtac ctttctggcg tttataactc tctttatcgg tgacgccggt
    11581 aatacaggga ataccacacc gacaaacgag gtattaacag acgatatttc agtgtccata
    11641 atttttctgt ccggttaaat aacgtcagtc tgctgccctc tgactgtaat catcaaagcc
    11701 ggaatgcata ccgggttaca acgtaaaaaa agtgatgctg gcccccggtg cagctggcgg
    11761 gcgaaacatg tttacctttc aataccccct gtctgcagtc tccactcttc catcctctta
    11821 agctacgtcg gccacatgaa tgcacttcca tgataacagg tattcctgtc agcataacga
    11881 agccggttac cttcccttcc tcctgcactt cctgtcataa tgactataca gcgcgcagat
    11941 tgccttttta ttgaccacct taatatacca ttataaactt ctttttatca tgttattatc
    12001 accttgttat gtttaatttt taagcaaccg gagaaagggg atggttcaga aaattttgtc
    12061 ggataaggtt atgaatgaaa gaacaaatgc gtattactct tattatcttg gagagcgtaa
    12121 tatctccgta ctgcccctta atgtttatga tcccccggaa cgttttatcg cttacataaa
    12181 aaaaaacaga gaaaatctga acatcacact ttcagatttt gaacttgaac aaataatttc
    12241 tggtatgcgt ctgaaggcac tggccttcct ggtccccctg gaaaaaatat cctggatcgc
    12301 gggcagtgaa cgcgcatgct tattttcctg gtatctgttg atgcagttta tccagaataa
    12361 cagggcaaaa ataagtgcag acttgcttca aaagaataaa ctgtatctta aagaagagta
    12421 tttagaaggg aatgccttcc cgtcggactc atcgacacag ttcagacaaa tactcagagt
    12481 tctcgatatt ctttctgata agaatttgcg ggatgagtgg ataatccaga ccaaagacag
    12541 atggatgcgg gcatttaaat caaagagccc tttcagttac ctcctcccgg agaacgaaca
    12601 tgaatgcata tggacatgga attatttaaa ggaaaaaaat attgcactgg acaacctggc
    12661 cagttttcct ggttctgctg atatttacca cgcaattcac ctttcatttg acatatgggt
    12721 tacccatcca tctgcctcac ctgacgatat aaaaaatttc aggaacagtt tcaataaggc
    12781 caaggcacag cgcaagtaca aaaaaatgca ggaagataaa gtcaatgtac agttttttct
    12841 tgacgcagaa accagggcgc agctcaaaga attatcgagg gccagaagac tcagtactgg
    12901 cgaaatgctg catgacctga ttgttgaaga gtataaaagg taccgacatt ccagataatt
    12961 tcagacatat attatctccg tgaatccccc gccacctttc cggtgtgtgg ggttttgtct
    13021 tttttcaccg ggaatacatg tatgaatccg tctgatgcca ttgaggcaat tgaaaaaccg
    13081 ctctcctccc tgccttactc actttcccgt cacatcctgg aacatctgcg taaactcacc
    13141 agtcacgaac ccgtgattgg cattatgggt aaaagcgggg ccggtaaatc ctcactctgt
    13201 aatacactgt ttcaggggga ggtcaccccg gtcagtgatg ttcacgccgg cacccgggaa
    13261 gtgcagcgct tccgtctgag tggccatggt cacagcatgg ttatcactga cctgcccggg
    13321 gtgggcgaga gtcgtgacag ggatgcagag tatgaagccc tgtaccgtga cattctgcct
    13381 gaactggacc tggtactgtg gctgattaaa gccgatgacc gggccctgtc tgtggatgag
    13441 tatttctggc gacacatcct gcaccgggga catcagcagg tgctgtttgt ggtgacgcag
    13501 gccgacaaaa cggagccctg ccatgaatgg gatatggccg gcattcagcc ttctcccgca
    13561 caggcacaga acattcgcga aaaaacggag gcggtattcc gtctgttccg gcccgtacat
    13621 ccggttgttg ccgtatcggc ccgcaccggc tgggaactgg atacgctggt cagtgcgctc
    13681 atgacagcgc ttcccgacca tgccgccagt cccctgatga cccgcctgca ggacgagctg
    13741 cgcacggaat ctgtccgcgc tcaggcccgt gaacagttta ccggtgcggt ggaccggata
    13801 tttgacacgg cggagagcgt ctgtgttgcc tctgttgccc gtacggttct gcgtgccgtc
    13861 cgtgacacgg tggtctctgt tgcccgcgct gtatggaact ggattttctt ctgaacagtc
    13921 tgccagggaa atgcctgata acattacgtt aaccaactga aaacatgaca caaacccctc
    13981 atactcctcc tgtttctctt gcgtgaccgc cttgtcatta ataggataaa acgatcgata
    14041 aaacaggtat cgatcgttac tatcgatcgt ttatagtggt taatggcctg cgaaaaggca
    14101 gctgaatctc ttcatcatgc agaacggaat tgcacacaac ggactgatac ttctctggcg
    14161 gatgagagag gggaagcatt tatgtccggg aaaaaccact ttatctgagc atgctgtttt
    14221 gtacctgccg gtatccacat ttgtgggtac cggctttttt attcaccctc tgtaaggaaa
    14281 agctgatgaa acgacatctg aacaccagct acaggctggt atggaatcac attacgggca
    14341 ccctggtggt ggcctccgaa ctggcccgct cacggggaaa acgcgctggt gtggcggttg
    14401 cgctgtctct tgctgctgtc acatcagtcc cggcactggc tgctgacaag gttgtacagg
    14461 cgggagaaac cgtgaacgac ggaacactga caaatcatga caaccagatt gtcctcggta
    14521 cggccaacgg aatgaccatc agtaccggtc tggagtatgg gccggataac gaggccaata
    14581 ccggcggaca atggatacaa aatggcggta tcgccaacaa cactactgtc accggtggtg
    14641 gtcttcagag agtgaatgcc ggaggaagcg tttcagacac ggttatcagt gccggaggcg
    14701 gacagagcct tcaggggcag gcagtgaaca ccactctgaa cggcggtgag cagtgggtac
    14761 atgaaggcgg gattgcaacg ggtaccgtca ttaatgagaa gggctggcag gccgtcaaat
    14821 ccggcgcaat ggcaaccgac acggttgtga ataccggcgc ggaaggggga ccagatgcag
    14881 aaaatggtga taccgggcag tttgttcgcg gaaatgccgt acgcaccacc atcaataaaa
    14941 atggtcgtca gattgtggct gctgaaggaa cggcaaatac cactgtggtt tatgccggcg
    15001 gcgaccagac tgtacatggt cacgcactgg ataccacgct gaatgggggg taccagtatg
    15061 tgcacaacgg cggtacggcg tctggcactg ttgtaaacag tgacggctgg cagattatca
    15121 aggaaggtgg tctggcggat ttcaccaccg ttaaccagaa aggcaaactg caggtgaacg
    15181 ccggtggtac cgccacacat gtcaccctga aacagggtgg agcactggtt accagtactg
    15241 cggcaacggt tcttggcagc aaccgtctgg gcaatttcac ggtggaaaac ggtaaggctg
    15301 acggcgttgt tctggagtcc ggcggtcgcc tggatgtact ggaaggccat tcagcgcaga
    15361 aaacacgggt ggatgacggc ggaaccctgg cagtgtctgc cggtggtaag gcaacaggtg
    15421 tcaccatgac atccggtggt gccctgattg cagacagtgg tgccactgtt gaggggacca
    15481 atgccagcgg taagttcagt attgatggca tatccggtca ggccagcggc ctgctgctgg
    15541 aaaatggcgg cagctttacg gttaatgccg ggggacaggc cagcaacacc actgtcggac
    15601 atcgtggaac actgatgctg gctgccgggg gaagtctgag tggcagaaca cagctcagta
    15661 aaggcgccag catggtactg aatggtgatg tggtcagtac cggcgatatt gttaacgcag
    15721 gggagattcg ctttgataat cagacgacac cggatgccgc gctgagccgt gctgttgcaa
    15781 aaggcaacgc cccggtaacg ttccataaac tgacaaccag taacctcacc ggtcagggtg
    15841 gcaccatcaa tatgcgtgtt cgccttgatg gcagcaatac ctctgaccag ctggtgatta
    15901 atggtggtca ggcaaccggc aaaacctggc ttgcgtttac aaatgtcgga aacagtaacc
    15961 tcggggtggc aacctccgga cagggtatcc gggttgtgga tgcacagaat ggcgccacca
    16021 cagaagaagg tgcgtttgcc ctgagtcgcc cgcttcaggc cggcgccttt aactacaccc
    16081 tgaaccgtga cagcgatgaa gactggtacc tgcgcagtga aaatgcttat cgtgctgaag
    16141 tccccctgta tacatccatg ctgacacagg caatggacta tgaccggatt ctggcaggct
    16201 cccgcagcca tcagaccggt gtaaacggtg aaaataacag cgtccgtctc agcattcagg
    16261 gcggtcatct cggtcacgat aacaacggcg gtattgcccg tggagccacg ccggaaagca
    16321 gcggcagcta tggcttcgtc cgtctggagg gtgacctgct gagaacagag gttgccggta
    16381 tgtctctgac gacaggggtg tatggtgccg caggccattc ttccgttgat gttaaggatg
    16441 atgacggttc ccgcgtcggc acggtccggg atgatgccgg cagcctgggc ggatacctga
    16501 atctgacaca cacgtcctcc ggcctgtggg ctgacattgt ggcacaggga acccgccaca
    16561 gcatgaaagc gtcatcggac aataacgact tccgcgcccg gggctggggc tggctgggct
    16621 cactggaaac cggtctgccc ttcagtatca ctgacaacct gatgctggag ccacaactgc
    16681 agtatacctg gcagggactt tccctggatg acggccagga taacgccggt tatgtgaagt
    16741 tcgggcatgg cagtacacaa catgtgcgtg ccggcttccg tctgggcagc cacaacgata
    16801 tgacctttgg cgaaggcacc tcatcccgtg acaccctgcg cgacagtgca aaacaccgtg
    16861 tgcgtgaact gccggtgaac tggtgggtac agccttctgt tatccgcacc ttcagttccc
    16921 ggggtgacat gagcatgggg acagccgccg ccggcagtaa catgacgttc tcaccgtccc
    16981 ggaatggcac gtcactggac ctgcaggccg gactggaagc ccgtgtccgg gaaaatatca
    17041 ccctgggcgt tcaggccggt tatgcccaca gcgtcagcgg cagcagcgct gaaggttata
    17101 acggtcaggc cacgctgaat gtgactttct gataatcagg tcactctcct tcaggccccg
    17161 gtcatcatga ccggggcttt ttactttcag ggagcaacag atatgccggg ttgtacatca
    17221 cgtttattgc ccgaaggccc tttctcacgc gaacaggctg ttgccgtcaa aacagcttac
    17281 cgcaatgtgt ttactgaaga tgaccaggga acatattccc ggctggttat ccgtaatgct
    17341 gaaggtcagt tgcgctggtg atgccggaac acagcttaat actcagctcg ccagttaagg
    17401 gcttctcaga caataaacgt cattcatttc atctgtcagg ccgcgtcttc tccgggagac
    17461 gtggcttttt catttattac accagtaaat cttaaccacc gataaggagc aaattatgcg
    17521 actagccagt cgttttggtt atacgaacca gatacgccgt gaccgtccgc tgacacacga
    17581 agaactgatg cactatgttc ccggtatttt cggtgaagac cggcatactt cccgcagtaa
    17641 acgatatgcg tacattccca ccatcaccgt actggaaagc ctgcagcggg aaggctttca
    17701 gccattcttc gcctgccaga cccgtgtgcg cgacccgggc cgccggggat acacaaaaca
    17761 tatgctgcgt ctgcggcggg ccggagagat aaacggagaa catgtccctg aaatcattct
    17821 gctcaactcc catgacggta cctccagcta ccagatgctg ccgggttact tcaggttcgt
    17881 ctgtcagaac ggatgcgtct gtggtcagtc tctgggggaa gtgcgtgttc cgcatcgggg
    17941 aaatgtggtg gacagagtca ttgaaggggc ttacgaggtg gtgggcgttt ttgaccggat
    18001 tgaggaaaag cgtgatgcca tgcagtcgct ggtcctgccg ccaccggcac gccaggcgct
    18061 ggcacaggcg gcgctgactt accgttatgg tgacgaacat cagcccgtca ccaccgccga
    18121 cattctgacg ccacgacgcc gggaggatta cggtaaggac ctgtggagcg catatcagac
    18181 catccaggag aatatgctga aaggcgggat ttccggtcgc agtgccagag gaaaacgtat
    18241 ccatacccgg gccattcaca gcattgatac cgacattaag ctcaatcgcg cattgtgggt
    18301 gatggcagaa acgatgctgg agagcctgcg ctgatgcagc ctgtccctga aggcgtgatt
    18361 caggtaccag aatcacccga cgccccggag gcgctgccgg ggtgtcgttc catttctgat
    18421 gatgacctgt gcacctggtg tacccgcctg ctttaccgtt ccggagagct cagcctgtgc
    18481 agactgagta caggcaatgg catctgacca tccgtctgtg accggaacgg ttacgcgtac
    18541 agctgtccgg aatttcagcc gaatatcatt catccctgat attaccgggt ctcacggtca
    18601 gtgatgaacg tgatgttgcc agctcccgac ctttactccc tctcatttat tcacattaca
    18661 aggatttcat acatgaaaac attgtctcag aacaccaccg cttcggcctg tgcaccggag
    18721 actggcctgc aacaactggt tgccaccatt gtccctgatg aacagcgcat cagcttctgg
    18781 ccgcagcatt ttggcctcat tccacagtgg gtcaccctgg aaccccgtgt cttcggctgg
    18841 atggaccgtc tttgcgaaaa ctactgcggt ggtatctgga atctgtacac cctgaacaac
    18901 ggcggggcat ttatggcacc cgaaccggat gacgatgatg acgaaacatg ggtactgttc
    18961 aatgccatga acggtaaccg cgctgaaatg agcccggaag ccgccggcat tgctgcctgt
    19021 ctgatgacgt acagtcatca tgcctgtcgt acggagtgtt atgccatgac ggtccattat
    19081 taccggctgc gggattacgc cctgcagcat ccggaatgca gcgccattat gcgcatcatc
    19141 gactgaatgg aggcagggac aatgcagcag ctttcctttc tgcccggaaa catgacgccc
    19201 ggcgagcgca gcctcattca gcgggccctg aaaaccctgg accgccatct tcatgaaccc
    19261 ggcgtggcct tcacctccac gcatgcggca cgggaatggc tgattctgaa catggcggga
    19321 ctggagcgtg aagaattccg ggtgctgtat ctgaacaacc agaatcagct gattgccggt
    19381 gaaaccctct tcaccggcac catcaaccgc acggaagtcc atccccggga agtgattaaa
    19441 cgcgccctgt atcacaatgc cgctgccgtg gtgctggcac acaatcaccc gtccggtgaa
    19501 gtcacaccca gtaaggcaga ccgccttatc acggaacgtc tggtacaggc actggccctg
    19561 gtggatatcc gggtaccgga ccatctgata gttggtggca accaggtttt ctcctttgcg
    19621 gaacacggtc tgctttaacc cgtcaccgtt acaaccacat cacacctgtt ttcactttta
    19681 tcttctgtct tcagaggtat cccactatga aaatcatcac ccgtgg
//
LOCUS       DACBVI010000082        19549 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.082, whole genome shotgun sequence.
ACCESSION   DACBVI010000082 DACBVI010000000
VERSION     DACBVI010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.082"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(132..878)
                     /locus_tag="IU325_14105"
     CDS             complement(132..878)
                     /locus_tag="IU325_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP5810142.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGVARVLTSGQKSDALQGLSK
                     IMELIAHGDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(892..1458)
                     /locus_tag="IU325_14110"
     CDS             complement(892..1458)
                     /locus_tag="IU325_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP5810143.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEKIVASEQSA"
     gene            1674..3407
                     /gene="argS"
                     /locus_tag="IU325_14115"
     CDS             1674..3407
                     /gene="argS"
                     /locus_tag="IU325_14115"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP5810144.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3460..3852)
                     /locus_tag="IU325_14120"
     CDS             complement(3460..3852)
                     /locus_tag="IU325_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HAP5810145.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGVTLNHRGESMSSAPLS
                     TRQPAAGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIPAIEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(3852..5930)
                     /gene="flhA"
                     /locus_tag="IU325_14125"
     CDS             complement(3852..5930)
                     /gene="flhA"
                     /locus_tag="IU325_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HAP5810146.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGTGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(5923..7071)
                     /gene="flhB"
                     /locus_tag="IU325_14130"
     CDS             complement(5923..7071)
                     /gene="flhB"
                     /locus_tag="IU325_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HAP5810147.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRIFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPQVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(7273..7917)
                     /gene="cheZ"
                     /locus_tag="IU325_14135"
     CDS             complement(7273..7917)
                     /gene="cheZ"
                     /locus_tag="IU325_14135"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HAP5810148.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(7928..8317)
                     /gene="cheY"
                     /locus_tag="IU325_14140"
     CDS             complement(7928..8317)
                     /gene="cheY"
                     /locus_tag="IU325_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheY"
                     /protein_id="HAP5810149.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(8332..9381)
                     /gene="cheB"
                     /locus_tag="IU325_14145"
     CDS             complement(8332..9381)
                     /gene="cheB"
                     /locus_tag="IU325_14145"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HAP5810150.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(9384..10244)
                     /gene="cheR"
                     /locus_tag="IU325_14150"
     CDS             complement(9384..10244)
                     /gene="cheR"
                     /locus_tag="IU325_14150"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HAP5810151.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10263..11864)
                     /gene="tap"
                     /locus_tag="IU325_14155"
     CDS             complement(10263..11864)
                     /gene="tap"
                     /locus_tag="IU325_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HAP5810152.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRGLQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDLVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(11910..13571)
                     /gene="tar"
                     /locus_tag="IU325_14160"
     CDS             complement(11910..13571)
                     /gene="tar"
                     /locus_tag="IU325_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HAP5810153.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(13716..14219)
                     /gene="cheW"
                     /locus_tag="IU325_14165"
     CDS             complement(13716..14219)
                     /gene="cheW"
                     /locus_tag="IU325_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HAP5810154.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14240..16204)
                     /gene="cheA"
                     /locus_tag="IU325_14170"
     CDS             complement(14240..16204)
                     /gene="cheA"
                     /locus_tag="IU325_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HAP5810155.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16209..17135)
                     /locus_tag="IU325_14175"
     CDS             complement(16209..17135)
                     /locus_tag="IU325_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein MotB"
                     /protein_id="HAP5810156.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17132..18019)
                     /gene="motA"
                     /locus_tag="IU325_14180"
     CDS             complement(17132..18019)
                     /gene="motA"
                     /locus_tag="IU325_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HAP5810157.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLATVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18146..18724)
                     /gene="flhC"
                     /locus_tag="IU325_14185"
     CDS             complement(18146..18724)
                     /gene="flhC"
                     /locus_tag="IU325_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HAP5810158.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(18727..19077)
                     /gene="flhD"
                     /locus_tag="IU325_14190"
     CDS             complement(18727..19077)
                     /gene="flhD"
                     /locus_tag="IU325_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HAP5810159.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4632 a         5441 c         4802 g         4674 t
ORIGIN      
        1 cacccgctca acagatcgcg gtaatcgttc attttttata ctattcaggg ataaaaaaag
       61 gcccctgttg aaattgcagg ggcctggtac gagcaagcat catattgggc gacatgatgc
      121 aacggtaaaa atcatttggc ctgatggcgt tcaatgattc ctttcatttc agcaaccgcc
      181 gccccgtcta cgacataacg cgaatactcg tccgcgtgtg catctgatga catggacaat
      241 ccttgattac gataacgcat tggtgaggct tgccacgctc ccgcggagct atggacttcc
      301 agcactccgg catcgaggaa gtggtgcagg ttttctgcac ggactcctgc tccggccata
      361 atgattggag catcaccatg ggcaataagt tccataattt ttgataaacc ttgcagtgcg
      421 tctgattttt gccctgatgt cagtacccga gcaacgccca attccgcaag attattgagt
      481 gtatttaaag ggttagcgca catatcgaag gcgcgatgaa aagtcactgc cagcggaccg
      541 gcagcagcca ttattttttc cattcgtggc atatcgacat tcccgtcaac atcgagaacg
      601 cccgtcacca gtccaggaaa acctaattcg cggaccgtgc gcacatcctc aagaatggcg
      661 gcaaactcac cgtcgctgta gcaaaaatca ccaccgcgtg ggcggataat cggatgcaca
      721 gggatcgtca cccgctggcg tacggatttc agtaccccca acgacggcgt taagcccccc
      781 tcttttgggg ctgcgcataa ttcaactctg tctgcgccgt tttgctgcgc cgttagtgca
      841 cattccatgc tgtaacagca aatttccagt aatgccattt ttactcctta attacgccga
      901 ctgctcgctg gcaacgatct tttcaatgga ccacggatga aacttaatag ttgttttgcc
      961 atcggtgacc gccagcgtcg gattcggtaa tcgctcatgc tcaccttttg gcgaactggt
     1021 tttcactgag atccccggca ggctgagtcc ttcatcagaa agcaatgcca gcgcacgggc
     1081 attcagcgtt tctggatcgc ccggcagaac gatttcaata tgttcccaac cttcgtgcgg
     1141 gtaacgtttt tccccgggcc acggtagctc cacaatggaa aactgccagt gcgcaacctg
     1201 taccggttca tgcaatttaa acagacaaat cggcctgcca ttgatcatat tttctgacaa
     1261 aagctcgccg cactgttcaa acccgcgacg ccagcgctca gcggtggcgt tttgatggca
     1321 acgcaaagaa atgtgatcgg cagtcagcgg agtgatatcc aaccccaaac ggcgggaaag
     1381 ttcatctaat gcgtgggtaa atcgcggtaa atccgatgca atatcctgca gctcatcgat
     1441 agattgccag ttcgccataa tcactcttcg tctttcagta aaagcgttaa tttaccctgt
     1501 tgccctgtgt caaccaaccg ctgatttcac gccgcttctg atgcaatagt gaaaacggca
     1561 atacgccacg cgcacgttgc tgacgaaaac agccatttgc agtatactcc cgccctaatt
     1621 tctttaactg gtgcgggcaa tttttgctcg cttcatcaat gtaaggtatt ccggtgaata
     1681 ttcaggctct tctctcagaa aaagtccgtc aggccatgat tgcggcaggc gcgcctgcgg
     1741 attgcgaacc gcaggttcgt cagtcagcaa aagttcagtt cggcgactat caggctaacg
     1801 gcatgatggc agttgctaaa aaactgggta tggcaccgcg acaattagca gagcaggtgc
     1861 tgactcatct ggatcttaac ggtatcgcca gcaaagttga gatcgccggt ccaggcttta
     1921 tcaacatttt ccttgatccg gcattcctgg ctgaacatgt tcagcaggcg ctggcgtccg
     1981 atcgtctcgg tgttgctacg ccagaaaaac agaccattgt ggttgactac tccgctccga
     2041 acgtggcgaa agagatgcac gtcggtcacc tgcgctctac cattattggt gacgcggcag
     2101 tgcgtactct ggagttcctc ggtcacaaag tgattcgcgc gaaccacgtc ggcgactggg
     2161 gtactcagtt cggtatgctg attgcgtggt tggaaaagca gcagcaagaa aacgccggtg
     2221 aaatggagct ggctgacctt gaaggtttct accgcgatgc gaaaaagcat tacgacgaag
     2281 atgaagagtt tgccgagcgt gcacgtaact acgtagtcaa actgcaaagc ggtgacgaat
     2341 atttccgcga gatgtggcgc aaactggtcg acatcaccat gacgcagaac cagatcacct
     2401 acgatcgtct gaacgtgacg ttgacccgcg atgacgtcat gggtgaaagt ctctacaacc
     2461 caatgctgcc gggtatcgtg gcggacctga aagccaaagg tctggcagta gaaagtgaag
     2521 gcgcgaccgt cgtattcctt gatgagttca aaaacaagga aggcgaaccg atgggcgtga
     2581 tcattcagaa gaaagatggt ggctatctct acaccaccac tgatatcgcc tgtgcgaaat
     2641 atcgttatga aacactgcat gccgatcgcg tgctgtatta catcgactcc cgtcagcatc
     2701 aacacctgat gcaggcgtgg gctatcgtcc gtaaagcagg ctatgtaccg gaatccgtac
     2761 cgctggaaca ccacatgttc ggcatgatgc tgggcaaaga cggcaaaccg ttcaaaaccc
     2821 gcgcgggtgg tacagtgaaa ctggcggatc tgctggatga agccctggag cgtgcacgtc
     2881 gtctggtggc agaaaagaac ccggatatgc cagccgacga gctggaaaaa ctggctaacg
     2941 cagttggtat tggtgcggtg aaatatgcgg atctctccaa aaaccgcacc acggactaca
     3001 tcttcgactg ggacaacatg ctggcgtttg agggtaatac cgcgccatac atgcagtatg
     3061 catacacgcg tgtattgtcc gtgttccgta aagcagaaat tgacgaagag caactggctg
     3121 cagctccggt tatcatccgt gaagatcgtg aagcgcaact ggcagctcgc ctgctgcagt
     3181 ttgaagaaac cctcaccgtg gttgcccgtg aaggcacgcc gcatgtgatg tgtgcttacc
     3241 tgtacgatct ggctggtctg ttctctggct tctacgagca ctgcccgatc ctcagcgcag
     3301 aaaacgaaga agtgcgtaac agccgtctga aactggcaca actgacggcg aagacgctga
     3361 aactgggtct ggatacgctg ggtattgaga cagtagagcg tatgtaatcg cgttatacgg
     3421 caaaagccga tgtcatggca gacatcggct tttttatcat taacgataat tcacaatcac
     3481 ttcattccgt tgtaccttta gcggcggaat taaccgccca ccgccaggca cttcccagat
     3541 aaatcgcaac ggttctatcg ccggtatacc ggaaaaggcc acggtggttc cactctgccc
     3601 ttctaattcg acacaacgag attgtgaaca caagcgaacc cgcagtcctg caggtgtcgg
     3661 gccgataagc tgataacgcc acgccaccag cgtcatcaat cctgcagcag gttgtcgagt
     3721 agaaagaggc gctgacgaca tcgactcacc acggtgattc agcgtaacgc ccacactact
     3781 cgcctgccac atcccctccc cggcggcttg caccagcagc ggaaacaata atattgctaa
     3841 taaggttctc atcatttacc gccaattgtc gccgtcatgc ggatatgtcg gttatcagat
     3901 agttccagat tcgacagcac cactaactgc ggcaagctgc ggcgcaggaa gcgagacaat
     3961 aatggtcgca gcgcgtggtt caccaataaa actggcggcg cacccaacat ctcctgacgg
     4021 gatagagctt cctgagtttg cgccagtaaa cgatccgcca gccctggctc cagtccgccc
     4081 ccgccctgca acgcctgtag caacaaacgt tccagcggtg tatcgaggcc aataacatga
     4141 acttcatctt tgccaggaaa ccactgctgg gtaatcgccc gtcccaacgc cacgcgcacg
     4201 acggcggtta attcatgtgg atcgctttgg atgggcgcat gttccgccag cgtttcgaga
     4261 atagtgcgca tatcgcgaat cggcactttt tcatcgagca gattttgcag cactttatgc
     4321 agtgtggtga gcgtgacgac gccaggaacg agatcttccg tcagctttgg catctcctgg
     4381 gcgacgcgat ccaacagctg ttgcgcctcc tgacgaccaa acagctctgc ggcatgctgg
     4441 ctaatgaggt ggttaagatg cgttgccacc accgtgctgg cctcaaccac ggtgtacccc
     4501 tgaatctgcg cctgttcttt aagcgcactt tcaatccaga tagcattcag gccaaatgcc
     4561 ggatcgacgg tcgcctcacc aggtaacgtc ccggcagcgg tacctgggtt aattgccagc
     4621 cagcgccccg gataggcatc gccagtgcca atctctacgc ctttcatcaa aatgcgataa
     4681 cgtgcaggtt gcagatccat attgtcgcga atgtgcacca ccggcggcag aaaccccatc
     4741 tcctgagcaa atttcttacg aatactacga atacggccca gcaactcgcc atcttgttgg
     4801 aaatcaacca tcgggatcag tcgataaccc acttccattc ccaaggaatc ttccagttgt
     4861 acatcgttcc acgtcgcttc gacaacggta ttattctctg ccatttttac cggttttggt
     4921 tcggcaggcg ctttttgttc acgtccgcgg atccaccagg ccagcccaag caatccggca
     4981 gtgaacagca aaaataccag gttcggcatt ccaggcacca ggccgagtaa accgagcacg
     5041 gcggcgctta acaacataac gctcgggtta ctgaaaagct gattcaccat ctgctcgcca
     5101 acatcctgat cggtgctgac acgcgtaacg atgacccccg cggcggtaga aatcaccagc
     5161 gccgggattt gtgccaccag accgtcgcca atggtcaata gcgtataact ttccgcggcg
     5221 tgtcccatgc tcatgccatg ttgcagcacg ccgaccagca acccgccgac aacgttaatg
     5281 accatgatga ggatcccggc gatggcatcg ccgcgaacaa acttacttgc cccgtccatt
     5341 gagccgtaaa aatcggcttc ctgagtcact tcggagcggc gttttttcgc ctcatcttca
     5401 ccaatcaatc cggcgttaag gtcggcgtca atcgccatct gcttacccgg cataccatcg
     5461 agaacaaagc gcgcccccac ttctgcgata cgcccggcac ctttggtaat gaccataaag
     5521 ttgatgatca cgagaatgac gaacaccacg ataccgatag cgaaattgcc accaacgagg
     5581 aagtgaccga acgcttcgac caccttccca gccgccgccg cgccggtatg cccttccatc
     5641 aaaatgatgc gggttgaagc tacgttaagt gccagacgca acaacgtagt aaacaataga
     5701 atggtcggaa acgcagcaaa ctcaagcgtg cgctgggtaa acatcgccac cagcaacacc
     5761 atgatcgaca aggcaatatt gaaggtaaac aacaggtcga gtatgaatgc gggcagtggc
     5821 agcaccatca tcgacaagat caacaggatc aaaatcggtc cggcaaggat ctgccattgt
     5881 gtcgatttca ggtttgcagg caggcgcagc atcgcggcca gattactcat gggtcggttt
     5941 ctcgttaata aaatccaggg cttccggcac cggaagatga gtaggttgta cagggcgctg
     6001 tccaccagcc agacgccagc gtttcagttg ccagacccag gccagcactt ccgccaccgc
     6061 ggcgtacagt tgacccggga tttgttgacc gatctccgca tgtcgataca gcgctcgcgc
     6121 cagcggcggc gcttcaagcg tagggacgtt attttcagcg ccaatttcac gaatgcgtaa
     6181 tgccaccagc cccgcgccct tggcaacgac ttttggcgca ctcattttgt tttcgtcgta
     6241 ctgcaaagcc actgagtagt gagttgggtt attgacgata acgtccgctt tcggcacatc
     6301 ggccatcatc cgacgccgtg cggccgctcg ctgcatctga cggatccgcc ctttaacttg
     6361 ggggtcgcct tcgctttgtt tgaactcatc acgaatatcc tgccgcgaca ttcgcagctt
     6421 tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc accatcggaa taacaccaag
     6481 taccaccagc agcgcgcata gccctaccag atccatcgca ttacccatgg cggtaatcgg
     6541 agactcggcc attaagcgca tcatctgcgg ccagtgatgc cagagaaaaa atcccgtcac
     6601 gctgccaacc aggatggttt tcaaaattgc cttaagcaac tccgcgccag tctgagccga
     6661 gaatatccgt ttaatgcccg gtagcgggtt gagtttggaa aacttcggct gcaaggattt
     6721 tccgctaaat accagccctc ccagcatgac cggagaaatg agcgccacca gcaccacgcc
     6781 gctaatcagc ggcagcagcg ccagcatggc ttctctgatc agcagaataa tttgcccgag
     6841 gatcagattc gggtcattga taatactgtg atcaaaatgc agcccagcgg agagcatgcc
     6901 cgacaatcga cgggccagcg acacaccgcc aaaccagata acactaacgc ccactaacaa
     6961 aatcagcagt gaagtcagtt cacgggaacg cgggatttgc ccctcttccc gcgctttttc
     7021 tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc tcgtcagaca cgtcgccaat
     7081 cctggaaaga gtattaagcg taaatgatgc cagagcgcaa agcgttcaat ggtttgagta
     7141 aggggcaaaa caggcgggat ttagggcttt tactgccaca catcaagcat agtatgcgtt
     7201 tgtcggatgc ggcgtcatcg cgttatacga ccgcctgata tgacgtagtc acgccacatc
     7261 aggcaataca aatcaaaatc caagactatc caacaaatcg tccacctgat cctgactggc
     7321 taccacaccg gctttgctgg tatcgacctg aggtccatta agcaaactct ggttttcacg
     7381 ttttggacgc gactcctgtt cggggatgtt ttccaacagc accatcagca actgacgttc
     7441 gatctcctga atgacatcca tcatccgctt aatgacctgt ccggtgagat cctgaaaatc
     7501 ctgcgccatc atgatttcca gcagctgcgc gttagtaaag ctggtatgcg cggggacatc
     7561 tgccagaaat tgccgcgtat ctgttaccag ttctcgggcg tcggcaaggt caatcggatc
     7621 ggcaaaccag tcatcccaac gttgggttaa cgcttttgct gatttctcca tttgatcctg
     7681 atgcggttgt gacgcctcaa cactgttcag cgcccgctcc gcagcctggg cggtcatctg
     7741 cacaacatag tacaaacgat cgcgcgcatc ggggatggct tccgccgctt cggcaatggc
     7801 ctgatccagc cccagctccc gcaaactgtc gcgcagcata cgcgtcaggc tgccgatgcg
     7861 cgcaatgata tcgccagctg aatgctcgtc agcaggtttg attgatggtt gcatcatagt
     7921 cgcatcctca catgcccagt ttctcaaaga ttttgttgag tttttcctcc agcgtcgcgg
     7981 cggtaaatgg cttcaccaca tagccactgg cccccgcttg cgccgcagca atgatgttct
     8041 ctttcttcgc ttctgcagtc accattaaca ctggcaatgc cgacatcgcg ccatccgcac
     8101 gaattgtttt cagcaattcc aggccatcca tattgggcat gttccagtcg gagataacaa
     8161 atccataacc gcctgcctgc aacttattga gagcgtcgac gccatcttcc gcttcctcaa
     8221 cattattgaa tcccagctct ttcagcaggt tacgcactat gcgtcgcatg gtggaaaagt
     8281 catccacaac caaaaattta agttctttat ccgccatttc acactcctga tttaaatacg
     8341 tatcgcctgt ccggcactaa tttttgccaa catttgctgg cttacctggc taagatcgac
     8401 cacttcgcag acaccaccca tattgatggc ctcgcgcggc atgccgaaca ccacgcaact
     8461 tgcttcgttt tgcgcaaggg tccatgcccc cgcctgacgc atcgccaaca ttcccgccgc
     8521 gccgtcgttg cccataccgg tcaggatcac cccaaccgca ttacgccccg cctgtttggc
     8581 gacagaatgg aacaacacat ctaccgaagg ccgatgacgg ttaaccgccg ggccatcgtg
     8641 aattttgatt tggtaatttg cgccactacg cgacagctcc atatgccgat cgcccggcgc
     8701 aatataggca tgccccggca agacacgttc tccgtcttcg gcttctttaa ccccgatctg
     8761 gcaaagctta ttaagtctgt cggcaaaaga gcgggtgaaa ccgggcggca tatgttgggt
     8821 aattaacaat gccgggctgg aaagcggcaa cggttgcagt acgtgacgaa ttgcctcagt
     8881 tccacccgtt gaagcaccaa tcgcaatcag tttttcagaa ctcaacaacg gacccgcctt
     8941 cagcgttgtc ggtgctgaca atggcttatg tgctgcaagg ctcgcctttg ctgccgtacg
     9001 caccttttca gcaatcattt cgctatacgc cagcattcct tcgcgaatac ccagttgcgg
     9061 tttggtgaca aaatctattg cccccagctc cagcgcgcgc agcgtgactt ctgatccttt
     9121 gccggtcagg gaagaaacca tcacaacggg cattggacgc aaacgcatta atttttcgag
     9181 gaaatccagt ccgtccatcc gcggcatttc aacatccagc gtcagcacat cgggattgaa
     9241 tttcttaatc aaatcacgcg cgaccagcgg atcaggcgcg gtcgccacca tttccatgtc
     9301 gctatggctg ttgatgattt ctgtcatgat ctggcgcatc agtgccgaat catcgacaga
     9361 taacaccctg attttgctca tcgttaatcc ttacttagcg catacaccgt ctgaccacgc
     9421 agcgtgaagc ggcgctcaag gtggctaaag ttttcagagt gacccgcaaa cagcaatccg
     9481 tcgggtttaa ggagcggaac aaagcggcgc aaaatctcct gctgggtatt ttgatcgaag
     9541 tagatcatga cgttacgaca gaagatcgca tcaaacggcc ccggcacggt gtactgtttc
     9601 gccagtagat tcagcggggc aaaatcaaca tagttcgcca gctcctgacg cacgcgtacc
     9661 agcccttcat gcggtcccgt ccctcgcatg aaataccgtt gcagttgctg cggcgtcagg
     9721 tttttcaact cttcatggcg ataaataccg cttctggctt tttccaacac ttcggtgtcg
     9781 atatcactgg caaacacttt ccagcgtccg ggcgcggtgc ccaatgtgtc agccagcgtc
     9841 atcgcaatgc tgtacggctc ttcgccggtc gaagccgccg cgctccatac gcgatactcg
     9901 ccagaacggc gacgtgcgtg atccgcgagc agagggaaat ggtgtgcctc acggaaaaat
     9961 gccgtcagat tcgtggtcag cgaattgata aacgcctgcc actcaccgct gtgctgatta
    10021 gattccagca agttcagata atgaccgaaa tccgtcagtc ccagcgaacg caaacgacga
    10081 accagtcggt tgtaaaccat gtcgcgttta tggtcagcca gaacgatccc ggctcgttga
    10141 tagatcaatt gacttatccg ccgaaaatgc gcgtcggaaa gcgccaggcg ctcggtcatc
    10201 tgtaacaata aagacgtttg cccacagggc agagatgaag tcatagcgcc ttctcaatca
    10261 cttcaggata ccactggcgc aatttgtaac tgcgccgact catgtcgtgc tacttcatgt
    10321 tcttcaaggg taaataccgc cacacgcgac gaaagatggt cggcctgatt cgccagttgt
    10381 tccgttgcca ccgccgcttc ttctaccagc gaggcgttct gctgagtcac cagatccatc
    10441 tggctgacag cctgtgcaac ctgctcaatc ccccgctgtt gttcttccga cgcagaggca
    10501 atttctccca taatgtcgtt cacgcgggtc accgaactga caatatcgat catggtcgcg
    10561 gcggcgttat tcaccagttt cgaaccctgc tggacacgat tgactgactc ttcgatcagc
    10621 cctttgatct cttttgctgc ctgcgcgcta cggctggcaa gattgcgcac ttcacctgcc
    10681 actaccgcaa aaccacgccc ctgctctccg gcgcgagccg cttccactgc cgcattcagg
    10741 gccagaatat tggtctggaa agcaattccg tcgataacgc tgataatgtc gccaattttt
    10801 tgcgagctgg tggcgatctc ctgcatggtg tgagtcatgg tactgacctg aacaccgccc
    10861 gcctgcgccg ttgtcgcggc attttttgcc agttccgacg cctgtcgtgc gttatcggcg
    10921 ttttgcccta ccgtggcggt taattgctcc atactggcgg ccgtttgtgc cagcgatgcc
    10981 gcctgctgtt cggtacgact tgagagatcg ttattgcctg cgacaatctc cgcgatacca
    11041 atgtgcatct cctggcttcc cttacgcaca tcacttaccg tcccacgcaa agcctgctgc
    11101 atggtcttca gactggcaaa aatggcggtg atctcattac gaccatatac cgcaatcgga
    11161 cgcgccagat tacccgcagc aatgctgtca aaatggctac cgataatggc cagtggttga
    11221 acaatcatct tgcgcgtcca ccacagcgca ctgctgatgt agatcgccgc aacaataatc
    11281 atgctgataa acaccagtgc cgaaatctga tagttacgct ggctttgcgc actggcggct
    11341 tccagcacat ggttgatctc cagctgccag gcctcaaagt taacgtcaaa cgcattctgt
    11401 gatccctgca ccggcgcagt gaggaaatcc gaaagttgat tactttcaag ccaggtcgcc
    11461 tgatgttcga gatcgttgtg ccagcgggca aaacttttct ccgtctcttt ttgcaatccc
    11521 ctgacgtgct cgttgcccgc agtcatcgcc ataaaacttt taaacagcgt ggtggattgc
    11581 gtcagactgg cgcgcgccgt cgtcatcaac gttttaatgt catccgccgg atagctaagt
    11641 gccgtcagag tgcccgcttt gttcagcgcg gtactggcct gtaacattac cgcccgagtt
    11701 tgcgctaatg ccgcacgttg ctgattgctc tgctcgacct gattcagtcg ttgcaaatcg
    11761 tcgcgaaatg cccaaaaaga catgccgtta ctgccaatct gcaagatccc gcagagaatc
    11821 aaaattaaaa acagcgtggt cgaaattcga atacgattaa acatcaacgc tccccatcag
    11881 gcggcaatga ccgcgttagt aaatactcgt caaaatgttt cccagtttgg atcttgttca
    11941 gcaattcgca gtcgtggctg tgccggtggt tgctcactgg caggacggga tggtgtttgc
    12001 ggtttattgg tgagtgggct ggctgccaga cggaacgcgg aaaccgcttg cgttaaacga
    12061 ctcgcctgtt cttccagcgc agcggcggcg gcagctgatt cctgcaccag cgatgcgttc
    12121 tgttgcgtga cgcgatccat ttccgaaacc gccaatgcga cttgatcgat gccacggctc
    12181 tgttcatccg atgccgatgc aatctcgccc ataatgtcag tcacgcgagt gacagcattg
    12241 acgatattgt tcattgtttc cccggcgctt tcgaccagca ccgaaccggt atcaacgcgt
    12301 gagacggagt cttcaatgag ggctttgatc tcttttgccg cctgggcgct gcgactggca
    12361 agattacgca cttcacccgc caccacggca aaaccacggc cctgttcacc cgcacgcgcg
    12421 gcttcaaccg cggcattcag cgcgaggata ttagtctgga aggcaatacc gtcgataacg
    12481 ctgataatgt cggcaatttt cttcgaacta tcggcgatct catgcatcgt tttcactacg
    12541 ccatccacca ctttgccgcc gtgctgggcg gtgtcggagg cactttgcgc cagttgcgag
    12601 gcctggcggg cgttatcggc gttttgcttc actgtcgcgg tgagctgctc catgctggcg
    12661 gcagtttctt ccagcgcgga tgcctgctgt tcagtacggg aggaaagatc ggtgttgccc
    12721 gccgcaattt cacgggtacc ggcatagatg gcatctgaac cttcgcggac atgagtgacg
    12781 gtgtcagtca aagagcgttg catatgtgaa acgctctgcg ccaggtcgcc catttcactg
    12841 cgcccgtcaa tggtcagggt attcgccagg ttaccaccgg cgatttcgcg aatgtgagca
    12901 ataatttttg ccagcggagt aagcaacata cggcgaatgc cgtaccacgc caccagcaga
    12961 atcaatacca ccaccagcgc gataaccgcc agttgccact gggcaaatcg gtaatcatct
    13021 gcgttgtcag tgacgatatc gcgatacagt ttttcactgc tgagggcgta ctgagcaaac
    13081 gcttcgccca ttgcattttg cattccctgg gttggctgag cgaaataagc tccagtattg
    13141 ccataatcta gataatcaat cagttcagtt aacgctgtgt aatagttttt atatttttca
    13201 tcaatattac gactggtagc gaccatttca ggtaacggtg ccatgctttt gaatttttta
    13261 taatgcgtcg ctgcctgcgc caatgttttc ctggcgctat cgagcaattc aactttggcg
    13321 ttactttgtt gattggagga atccatcatc atccgtaccg ctgaacgact caggttaatg
    13381 cgcgtttgca gcattaaatc ccaggttgac gtcagctcgc cctgctgttc ccgtaattga
    13441 ttggaaacca caaagctctt ctggctatgg tgaagggaag aaaaaaacag actgccggaa
    13501 ataagctgta acagtgcgaa tacccccagc accattacca acagcgtgac tacgcggata
    13561 cggttaatca taaggcacct tcctgaaaac aagttgatct cgttatcggc aaggaggggg
    13621 gaaactttat tgctgatgcc acccgccgcg aaattgaaat aaaaaacccg atgcgcagat
    13681 catcgggttc atttcaattg aggaaatcgg gagaattacg ccacttctga cgccgcgcta
    13741 tctaacagcg ccatctcttc gctgttcagc agtttttcga tgttcaccag aatcaacatc
    13801 cggtcgccca gtgcgcccag tccagtgaga tattctgttg aaagcgtcac ggcaaattcc
    13861 ggtgccggac gaatttgctc cgccgtcaat gaaagcacgt ctgagacgcc gtcaaccacg
    13921 atgccgacca cccgctgtcc gagattcagg acgataacta ccgtgttgtc gttatagtcc
    13981 acatccacct ggctgaactt aattcgtaag tcaacaatcg gcacaataac gccgcgcaga
    14041 ttcgtgacgc ctttgataaa cgctggcgtg ttcgcaatcc gtgttacctg atcgtagcca
    14101 cggatctcct gcactttcag gatatcaata ccgtactctt catcaccaag ggtaaatacc
    14161 agaaattcct ggcctgacgg ctcgctggcc agctttgtta cattcgtcat accggtcata
    14221 ttgttacctt tttactcatt caggcggcgg tgttcgccat acgttgttcg cggtttatcg
    14281 cctgcaaggc ggagacatca acaatcagtg ccacgctgcc gtcgccaaga atggtcgcag
    14341 cagaaatgcc ggggactttg cgatagttac tttcaaggtt tttaaccaca acctggtgtt
    14401 gaccaattaa ttgatccacc agcaaggcgt agcggcgacc gccactttgt aagatcacca
    14461 caattccctg ggtggcttcg gttttcgcgc ccgcgacgtt gaacactttc cacagttcga
    14521 cgatgggcag atattcaccc cgcacttcca gcacccgctc gccgccggcc agtggatgga
    14581 gatcggcttc acggggttgc agtgattcca taacagcatt cagcggcaga atgaaaactt
    14641 catccgcaac gcgtacggac atgccgtcga ggatggccag cgtcagcggc agtaaaatgc
    14701 ggatcgtagt gccagtaccc tgcttcgact ggatttcgac atgaccgccc atctcctgga
    14761 tattacgttt aacgacgtcc atgccgacgc cgcgcccgga gacgtcggtg acctgctctg
    14821 ccgtggagaa gccaggtgca aatatcagca tcgcgacttc gtcgtcgctc atgttttcgc
    14881 tgacagtcaa accttgcgag gccgcttttg ccagaattcg ctcacggttt agccccgccc
    14941 catcgtcggt cacttcaatg caaatgttgc cgccctgatg ttcggcagac agaattaaat
    15001 ttccgacgct gtttttacct gcggcgagcc gtttttctgg cagttcaata ccgtgatcga
    15061 ggctattgcg taccaggtgg gtcagcgggt cgataatgcg ttctatcagg ctcttgtcga
    15121 gctcggtgga actgcccacc agcgtcagtt ctacctgctt gccgagtttt cccgccagat
    15181 cacgcaccag ccggggatag cgactaaaga catattccat cggcatcatg cgaatcgaca
    15241 tcactgattc ctgcaaatca cgggcgttac gttgtaactg ccccatgctg gtaatcaaat
    15301 caccatgatt aaccgggtcc agttcgctgg aacgctgggc aagcatggac tgggtgataa
    15361 ccagctcgcc gacgaggtta attaattgat caaccttttc taccgctaca cggatgctgg
    15421 tggattcact gctgcgcgtc gttttttccc gctccacgcg gccggttggc gcttgttcgg
    15481 ctgccagttt aagcacaggt ggggtagcta tttttggcga gacttctact gtttcaaagg
    15541 taatctgatc ggcttcaatc acaaaacaga gtaccgctgt gatgtcatct tcggcgatgt
    15601 cgcccggtaa tattgccgag agcgaatccg cccctttcac cacgtcagtt aaggttgtca
    15661 gatgtcccag ttcttcttcc agcaggtcga cttccccggc cttcaggcgc gaaaggataa
    15721 ttcgtcgcgg cgactgactg cgactctgct catcttgcgg ttcacttttg gcaaccacac
    15781 ttaatcgggt cactgcggat ggcgtttcgc cttttgcttc taatgccagt tgacgcaagg
    15841 cctggcagat ataatcgaag ctggcggcat ccggctcttg cgactgttta taagcgtcga
    15901 gctgttcttg catgatgtcc ttcgtttcca aaaacagatt gataatgtcg gtgttgagtt
    15961 gcatctcacc tcgtctggct tcatcgagca ggttttccat cagatgcgtg gtttcctgca
    16021 aaacgctgaa gccaaaagtt cctgcccctc ctttgatcga gtgggcagcc cgaaagatgg
    16081 cattcaattg ttcggcatct ggcgcttccg gctgcaaaac cagcagatgc tgctccatat
    16141 cagccaacag ttcgtccgct tcatcaaaaa atgtctgata aaaatcgctt atatccatgc
    16201 tcacgctgtc acctcggttc ggctgatggc attgtgggaa cactgacctg tggtgcaacc
    16261 tcaggttttt ccagggcgct tactggctca ttctggcttt cggcgttttc atgcaaaatg
    16321 gcctgttcgg cttgtttgtt cagtaccagc aggctgatgc gacggttgac ggcatcatca
    16381 ggtccgcgat cgcttaagcg catcgttgcc gccatgccga cgacacgtaa cactttgccg
    16441 ctatccaacc ctccgaccat cagttcgcgg cgggatgcat tggcccgatc ggcagaaagc
    16501 tcccagttgc tgtaaccttt ctcaccactg gcgtagggga aatcatcggt atgacctgaa
    16561 aggctaatac ggttgggaat accgttcagt acaggcgcaa tggcgcgcag aatgtcgcgc
    16621 atatagggtt cgacatcggc actgccggtt ctaaacatcg ggcgattctg gctatcgatg
    16681 atctgaatac gtagaccttc ctggaccaga tcgattttga gatggggacg taacgcccgc
    16741 agtttcggat cggactctat caactggtcg agatcacccc gcaatttccg caatcgactt
    16801 tgctccatgc gttttttcag ctcttcgatg ttcggctgct tattcacttc cccctggctt
    16861 tgggtgtaat catcaccacc gccgggaatt gggctttcac tattagaaat gcgatcgccg
    16921 cccgtaaccg cagtcgccag tggagtccgg aagtactccg caatctgaat cagctctttt
    16981 gggctggaga tggagatcag ccacatcacc agaaaaaagg ccatcatcgc agtcataaag
    17041 tcggcataag caatcttcca cgatccatgt gctgccccgt ggcttttggc tttgcgtcgt
    17101 ttgacgacaa taatcggatg cgcttgattc ttcatgcttc ctcggttgtc gtctgttgtt
    17161 gcggattttt caccgcacgc acatgctctt ccagttcaat aaacgacgga cgttcgctgg
    17221 aatagagcgt tttgcgacca aactcaacgg cgataggcgg tgcgtaaccg ttcagattag
    17281 aaagcagagt gactttgacg cactgcatca ttttgctggt ttcggcgctt ttctgacgta
    17341 aaacagtcgc taatggggaa ataaatccgt aagccaataa aatgccgagg aaagtcccca
    17401 ccatcgcatg tgcgataagc gcacccagct cggcggcagg acgatcggct gaacctaacg
    17461 cgtgaacgac ccccattaca gccgcaacaa taccaaacgc cggaagtgag tccccgacca
    17521 gcgccagact gttcgccggg acttctgcct cgctttcgtg cgtctcaatc tcttcatcca
    17581 tcagagcttc gatttcgaag gtgttcatgt gaccgctgat aatcaggcgc agataatcga
    17641 cgataaaatc aagcatgacg ctatccgcga ggatgcgtgg gtagctggcg aagatctcgc
    17701 tctcacgggg attttcaata tcacgttcca gcgaaaacat tcccatctgc cgcgatttcg
    17761 ccatcaaccg ataaagcaga gccagcagat ccatatacat tgctttggtg tatttggagc
    17821 gacgaaacag caacggcagc gccttcagcg tgcctttaat cgctttgcca ttattgccga
    17881 cgataaacga cccaatccct gcaccggcaa taatcaccag ttcagcgggt tgatagagtg
    17941 ctccaaggct tccaccggtc atcaaataac cgccgaaaac tgtaccgaga acaaccaggt
    18001 aacctaataa gataagcacg acatcatcct tccactgttg accatgacag gatgttcagt
    18061 cgtcaggcgt taacgcgcga ttggggcaaa aaaaagcagc ggtacgtcgt taccgctgct
    18121 ggaatgttgc gcctcaccgt atcagttaaa cagcctgtac tctctgttca tccagcagtt
    18181 gtgggataat atcggcagga ttctgggaaa gtttacgtct ttttactgcc cgggatggcg
    18241 gttgacataa gctgcaggca aagctgccaa caggctggtg agcgtgggta ataaaattgc
    18301 cgccgcagca gttgcagctg gaaagttgca gtaatccact ttcaacaaac cgcaccaatg
    18361 tccaggcacg ggttaatgcc agcagtggtc cttcttctgc ttgtgggcac tgttcaaggt
    18421 ataaacggta ggctttgatc accgcatcga cgccattaca caaaccggtt ttcagtaaaa
    18481 actgccatgc attacagaac atcgaagcat gaacgttttg ttcccaggtc ataaaccagt
    18541 cggttgagaa tggcagcatg cctttcggcg gtgggcttcc gcgcagttct ttataaagtt
    18601 ttatcaggcg tccgcgactt aactgtgttt cgctttccag catctgcaaa cgagcgccca
    18661 gggtgatcaa ttccattgcc agctgaatat cccgcgcttc ctgaacaatg cttttttcac
    18721 tcatgatcag gcccttttct tgcgcagcgc ttcttcaggc tgattaacat cattcagcaa
    18781 acgtgttgag agcatgatgc cggtatgaat ttgctggaga tcgtcaacgc gggaatcttg
    18841 cgtcaactga gtaatcgtct ggtggctgtc aaaacggaag tgacaaacca gttgattggt
    18901 ttctgccagc ttaaccattt gcggaagagt cagtgccgct aacgttgtcg ccatttcttc
    18961 atttatgccg agacgaaaca tagcggacgc tttgtcctga acaatcaaac gctgtgcaag
    19021 tagtaaatat gacaagttga tgtcataaat gtgtttcagc aactcggagg tatgcattat
    19081 tcccacccag aataaccaac tttattttta tgcggtttca ccgcaccccg tgatgtcgcc
    19141 gggaagcccc ggtaaaaaat aattagcatt agaatagttg cgataagctg caataagcag
    19201 aaccaccttt ttggtttaat atgtccttac aaatagaaat gggtctttac acttatctaa
    19261 gatttttcct aaatcgacgc aactgtactc gtcactacac gcacatacaa cggagggggg
    19321 ctgcgatttt caataatgtg tgatgcagat cacacaaaac actcaattac ttaacataaa
    19381 tgtataagtc atacttttgt tttgggtgta tttcaatctg ttaaaaagtt tttcgctacg
    19441 ctagcaagca aaaatgaaac aggaataatc gaaatgggat gttgcgcaca gtcaaaataa
    19501 ctcaccgtaa ataatcatct gctataaata atcactttca tgcaatacc
//
LOCUS       DACBVI010000083        19247 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.083, whole genome shotgun sequence.
ACCESSION   DACBVI010000083 DACBVI010000000
VERSION     DACBVI010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.083"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            284..2959
                     /gene="adhE"
                     /locus_tag="IU325_14195"
                     /gene_synonym="adhC"
     CDS             284..2959
                     /gene="adhE"
                     /locus_tag="IU325_14195"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP5810160.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            complement(3262..3879)
                     /locus_tag="IU325_14200"
     CDS             complement(3262..3879)
                     /locus_tag="IU325_14200"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP5810161.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALEVGSLTAIQERHRHD"
     gene            4483..4896
                     /locus_tag="IU325_14205"
     CDS             4483..4896
                     /locus_tag="IU325_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HAP5810162.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(5041..5949)
                     /gene="galU"
                     /locus_tag="IU325_14210"
     CDS             complement(5041..5949)
                     /gene="galU"
                     /locus_tag="IU325_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP5810163.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(6151..7164)
                     /gene="rssB"
                     /locus_tag="IU325_14215"
     CDS             complement(6151..7164)
                     /gene="rssB"
                     /locus_tag="IU325_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HAP5810164.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(7256..8161)
                     /gene="rssA"
                     /locus_tag="IU325_14220"
     CDS             complement(7256..8161)
                     /gene="rssA"
                     /locus_tag="IU325_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HAP5810165.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            8274..8732
                     /locus_tag="IU325_14225"
     CDS             8274..8732
                     /locus_tag="IU325_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HAP5810166.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMK
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDVDNIGFVSFVARYTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            8782..9624
                     /gene="purU"
                     /locus_tag="IU325_14230"
     CDS             8782..9624
                     /gene="purU"
                     /locus_tag="IU325_14230"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HAP5810167.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            9783..9867
                     /locus_tag="IU325_14235"
     tRNA            9783..9867
                     /locus_tag="IU325_14235"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9817..9819,aa:Tyr,seq:gta)
     gene            9902..9986
                     /locus_tag="IU325_14240"
     tRNA            9902..9986
                     /locus_tag="IU325_14240"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9936..9938,aa:Tyr,seq:gta)
     gene            10033..10164
                     /locus_tag="IU325_14245"
     ncRNA           10033..10164
                     /ncRNA_class="other"
                     /locus_tag="IU325_14245"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            10211..10342
                     /locus_tag="IU325_14250"
     ncRNA           10211..10342
                     /ncRNA_class="other"
                     /locus_tag="IU325_14250"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            10390..10521
                     /locus_tag="IU325_14255"
     ncRNA           10390..10521
                     /ncRNA_class="other"
                     /locus_tag="IU325_14255"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(10706..11383)
                     /gene="narI"
                     /locus_tag="IU325_14260"
     CDS             complement(10706..11383)
                     /gene="narI"
                     /locus_tag="IU325_14260"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HAP5810168.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(11383..12093)
                     /gene="narJ"
                     /locus_tag="IU325_14265"
     CDS             complement(11383..12093)
                     /gene="narJ"
                     /locus_tag="IU325_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HAP5810169.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLCARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(12090..13628)
                     /gene="narH"
                     /locus_tag="IU325_14270"
     CDS             complement(12090..13628)
                     /gene="narH"
                     /locus_tag="IU325_14270"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAP5810170.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(13625..17368)
                     /locus_tag="IU325_14275"
     CDS             complement(13625..17368)
                     /locus_tag="IU325_14275"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAP5810171.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPIEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLLGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     ILLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAKKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATFERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKELGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(17762..19153)
                     /gene="narK"
                     /locus_tag="IU325_14280"
     CDS             complement(17762..19153)
                     /gene="narK"
                     /locus_tag="IU325_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HAP5810172.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWV
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         4923 a         5085 c         4613 g         4626 t
ORIGIN      
        1 tataatggcg aaaagcgatg ctgaaaggtg tcagctttgc aaaaatttga tttggatcac
       61 gtaatcagta cccagaagtg agtaatcttg cttacgccac ctggaagtga cgcattagag
      121 ataataactc taatgtttaa actcttttag taaatcacag tgagtgtgag cgcgagtaag
      181 cttttgattt tcatcggtta agcaaatcat caccgcactg actatactct cgtattcgag
      241 cagatgattt actaaaaaag tttaacatta tcaggagagc attatggctg ttactaatgt
      301 cgctgaactt aacgcactcg tagagcgtgt aaaaaaagcc cagcgtgaat atgccagttt
      361 cactcaagag caagtagaca aaatcttccg cgccgccgct ctggctgccg cagatgctcg
      421 aatcccactc gcgaaaatgg ccgttgccga atccggcatg ggtatcgtcg aagataaagt
      481 gatcaaaaac cactttgctt ctgaatatat ctacaacgcc tataaagatg aaaaaacctg
      541 tggtgttctg tctgaagacg acacttttgg taccatcact atcgctgaac caatcggtat
      601 tatttgcggt atcgttccga ccactaaccc gacttcaact gctatcttca aatcgctgat
      661 cagcctgaag acccgtaacg ccattatctt ctccccgcac ccacgtgcaa aagatgcaac
      721 caacaaagca gctgatatcg ttctgcaggc tgctatcgct gccggtgctc cgaaagatct
      781 gatcggctgg atcgatcaac cttcggttga actgtctaac gcactgatgc accacccaga
      841 catcaacctg atcctcgcga ctggtggtcc gggcatggtt aaagccgcat acagctccgg
      901 taaaccagct atcggtgtag gcgcgggtaa cacgcctgtt gttatcgatg aaactgctga
      961 tatcaaacgt gcagttgcat ctgtgctgat gtccaaaacc ttcgacaacg gcgtaatctg
     1021 tgcttctgaa cagtctgttg ttgttgttga ctctgtttat gacgcagtac gtgaacgttt
     1081 cgcaacccat ggcggctatc tgttgcaggg taaagagctg aaagctgttc aggacgttat
     1141 cctgaaaaac ggtgcgctga acgcggctat cgttggtcag ccagcctata aaattgctga
     1201 actggcaggc ttctctgtac cagaaaacac caagattctg atcggtgaag tgaccgttgt
     1261 tgatgaaagc gaaccgttcg cacatgaaaa actgtccccg actctggcaa tgtaccgcgc
     1321 taaagatttc gaagacgcgg tagaaaaagc agagaaactg gtagctatgg gcggtatcgg
     1381 tcatacctct tgcctgtaca ctgaccagga taaccaaccg gctcgcgttt cttacttcgg
     1441 tcagaaaatg aaaacggctc gtatcctgat taacaccccg gcttctcagg gtggtatcgg
     1501 tgacctgtat aacttcaaac tcgcaccttc cctgactctg ggttgtggtt cctggggtgg
     1561 taactccatc tctgaaaacg ttggtccgaa acacctgatc aacaagaaaa ccgttgctaa
     1621 gcgagctgaa aacatgttgt ggcacaaact tccgaaatct atctacttcc gccgtggctc
     1681 cctgccaatc gcgctggatg aagtgattac tgatggccac aaacgtgcgc tcatcgtgac
     1741 tgaccgcttc ctgttcaaca atggttatgc tgatcagatc acttccgtac tgaaagcagc
     1801 aggcgttgaa actgaagtct tcttcgaagt agaagctgac ccgaccctga gcatcgttcg
     1861 taaaggtgca gaactggcaa actccttcaa accagacgtg attatcgcgc tgggtggtgg
     1921 ttccccgatg gacgccgcga agatcatgtg ggtcatgtac gaacatccgg aaactcactt
     1981 cgaagaactg gcgctgcgct ttatggatat ccgtaaacgt atctacaagt tcccgaaaat
     2041 gggcgtgaaa gcgaaaatga tcgctgtcac caccacttct ggtacaggtt ctgaagtcac
     2101 tccgtttgcg gttgtaactg acgacgctac tggtcagaaa tatccgctgg cagactatgc
     2161 gctgaccccg gatatggcga ttgtcgatgc caacctggtt atggacatgc cgaagtccct
     2221 gtgtgctttc ggtggtctgg acgcagtaac tcacgccatg gaagcttatg tttctgtact
     2281 ggcatctgag ttctctgatg gtcaggctct gcaggcactg aaactgctga aagaatatct
     2341 gccagcgtcc taccacgaag ggtctaaaaa tccggtagcg cgtgaacgtg ttcacagtgc
     2401 agcaactatc gcgggtatcg cgtttgcgaa cgccttcctg ggtgtatgtc actcaatggc
     2461 gcacaaactg ggttcccagt tccatattcc gcacggactg gcaaacgccc tgctgatttg
     2521 taacgttatt cgctacaacg cgaacgacaa cccgaccaag cagactgcat tcagccagta
     2581 tgaccgtccg caagctcgcc gtcgttatgc tgaaattgct gaccacctgg gtctgagcgc
     2641 accgggcgac cgtactgctg ctaagatcga gaaactgctg gcatggctgg aaacgctgaa
     2701 agctgaactg ggtattccga aatctatccg tgaagctggc gttcaggaag cagacttcct
     2761 ggcgaatgtg gataaactgt ctgaagatgc attcgatgac cagtgcaccg gcgctaaccc
     2821 gcgttaccca ctgatctccg agctgaaaca gattctgctg gatacctact acggtcgtga
     2881 ttatgtagaa ggtgaaactg cagcgaaaaa agaagccgct ccggctaaag ctgagaaaaa
     2941 agcgaagaaa tccgcttaat cagtagcgct gtctggcaac ataaacggtc ccttctgggg
     3001 ccgttttttt gttttaccca aagcaacttt cccataaacc gacagcattt gccttcatca
     3061 tatttacggc gatgtataac gcctacacac agcgatattg tactttacag gtcacaagtc
     3121 aatgtcactg taaaagcgct ctgtgagggg tatagtggcg ttaaagaact gtcgagaagg
     3181 gtatatacac cggatgaagt gagtaaaacg aactgacagg ataaaagcgc cctgctcacc
     3241 ctgtcagtaa agaaattctt attaatcgtg gcgatgcctt tcctgaatag ccgttaatga
     3301 gccgacttct aacgcttctt tatagtgttt acggcataca gaaacgtagc gttcattacc
     3361 accaataacc acctgctcac cttcgttata aggtctgcct gcttgatcaa gacgcagcac
     3421 catgcttgct ttgcggccac aaaaacagat ggtttttaat tcaaccagtt tgtcggacca
     3481 tgccagtaag tattggctgc caataaataa ctcacctcga aaatcggtac gtaaaccata
     3541 acaaagtacg ggtatatcga gttgatcgac aacctccgat aattcatata cttgttgtct
     3601 ggttaaaaac tggcattcat caaccagtac gcaatgaatt gcctgctgtt catgttccgc
     3661 acgaatctca tcaaataatg atgaattttg gttaaataat tttgcaggcg atgacaaacc
     3721 tatacgcgag ctgactttcc cggcaccaaa gcgatcatca atttctgccg tatatacgac
     3781 agtgcgcatg ccgcgttcct ggtaattgta tgaagattgc aacaatgctg tagacttacc
     3841 cgcattcatt gcggaatagt agaaatatag ctgtgccatc agccacaggc cctcaatgat
     3901 atgaattaaa gttggctgga gtttatcata attcgtcagt ttttcagctc aacagtatgc
     3961 acagaagtgg tatatgcgtt ctcccttacg aagccttgca taatccttct gagctatcat
     4021 tacaactgcc tcgcttgtta taagcgggta aatgactgct ggtaactatt cacaatcttt
     4081 aacctgttgc gcaagtaata gccctctgtt gacctcctgg agatagtgca atactaagtc
     4141 cctgctctta ttgcgactgt tctacttttc atcattcgct taatagggaa ttctcgtaaa
     4201 cacgactaat acagaagact gaaaggtcgt cagcctacga taatctcccc ataaaatgtg
     4261 acatgaatca ggaagtttta acctcacgtg ctgcgaaatc atcggcgcaa atagggctat
     4321 atgccgcgtc ttttctggct aattttatga aaagatattt attggcggca caaaataaag
     4381 aacaattttg aattccttac attcctggct attgcacaac tgaatttaag gctctattat
     4441 tacctcaaca aaccacccca atataagttt gagattacta caatgagcga agcacttaaa
     4501 attctgaaca acatccgtac tcttcgtgcg caggcaagag aatgtacact tgaaacgctg
     4561 gaagaaatgc tggaaaaatt agaagttgtt gttaacgaac gtcgcgaaga agaaagcgcg
     4621 gctgctgctg aagttgaaga gcgcactcgt aaactgcagc aatatcgcga aatgctgatc
     4681 gctgacggta ttgacccgaa cgaactgctg aatagccttg ctgccgttaa atctggcacc
     4741 aaagctaaac gtgctcagcg tccggcaaaa tatagctacg ttgacgaaaa cggcgaaact
     4801 aaaacctgga ctggccaggg ccgtactcca gctgtaatca aaaaagcaat ggatgagcaa
     4861 ggtaaatccc tcgacgattt cctgatcaag caataatctt ttgtagattg cacttgctta
     4921 aaaatcccgc catcggcggg attttttatt gtccggttta agacaattta ataagaataa
     4981 gctataaaaa aacggcgtcg attgctcaac gccgtttcgt ggataacacc gatacggatg
     5041 ttacttctta atgcccatct cttcttcaag ccaggcttta aattccgtgc caagggtgtt
     5101 atgacgaata ccgtattcaa cgaaggcctg catgtaacct aatttattac cgcagtcatg
     5161 gctcttccct ttcatatgat aggcttccac cgtttctttt tcgatcagca tatcaattgc
     5221 gtcggtgagc tgaatttcat caccagctcc cggaggggtt tttgccagca acggccaaat
     5281 atccgcgcta agtacgtaac gacccacaat agcgagatta gacggcgcaa catccgcttt
     5341 cggtttttct accacaccaa ccatcggtac gctttcaccc ggcgctaatt caacgccttt
     5401 gcaatccaca acgccatatg cggtaacatc agcaaccggt tcaaccatga tctggctatg
     5461 acccgtttca tcaaagcggc ggatcatctc tgccaggtta tcctgtgaca aatcggattc
     5521 atattcatcc agaataacgt caggcaaaat aacagccaca ggttcatcac ccactaccgg
     5581 gtgagcacac aataccgcat gtcccaggcc tttcgccagc ccctgacgaa cttgcataat
     5641 agtcacgtgc ggcggacaaa tagactgcac ttcatcaagc agttgacgtt ttacacgttt
     5701 ttccagcatt gcttccagtt caaaactggt atcaaagtgg ttttcaatcg agtttttaga
     5761 tgagtgagta accagcacaa tttcagtaat gccagccgca atacattcat tcacgacgta
     5821 ttgaattaat ggcttatcga caagtggcag catctctttc gggatggctt tcgtcgccgg
     5881 caacatcctg gttcctaatc ccgcaacggg gataacggct tttttgactt tcgtattaat
     5941 ggcagccatt taaattctcc tggactgttc gtgttttgaa cgtgttcata tttctgtatc
     6001 gcatcccagt atatcagcac cccgaagggg ctaagttctg aaaaggaggc gagttagcat
     6061 aattaagaca attacttata cgaatggcga cgatagtaac accagccgac attagcaggt
     6121 aatgcaaatt tagcccgcgt tatcgtttgc tcattctgca gacaacatca agcgaagccg
     6181 accaccggtt ccccatattt ggcattgcca ggcatcgcat cgctggctca attgattcaa
     6241 ataagcgtta cctaaagtgc ctaacggaac accattactg atttgcacct ggtgttcgcc
     6301 agtatttaat gtcgcattca gacccgcaga aaccagaatg agatttttca gttcgcgatg
     6361 ataatagcca actaatagcg gaaactgccc cggtagattg gcctgacgga gtaaatggtt
     6421 tacctgcttc aataacgcgc ccaactctgg caaccgttga ttttgatgtg caagctgttc
     6481 ctgtaataat ccgttaaaca atgcgcgtaa taacaaggca gcaagtacgc cattatgtcc
     6541 agctcgggtg acatcaaggc aataaaatgc cagatcgttt tccgaaagtg cggcaatatc
     6601 aagcaccagg ccgggtttgt ccgcggcaac caattgacga taattaaccc ggcaatggga
     6661 aatcacctgc tgaaccggcg gttgcaattc ctgtaataat ttcgccgctg cggcagggtt
     6721 atcaaccatt gcatcccagt cgcgaaaaag cctctcctct tcctcaacgc gggaattaaa
     6781 catgctggga tagagacagg caaaaaccat ctcgcgcaag cgattcagat ctttaactgg
     6841 tttcagcaaa acatcttcaa cgcccagacg taacgctttg gcaatatctg ccatattttc
     6901 agtggcagat atcaccagaa caggtgtctg gtcgcctctg ttacgtatat gctccagcag
     6961 tttaagcccg ttcattcgtg gcatcgcgat atcacatatc atcaggtctg gagtgaaacc
     7021 tcccagcaac tcaagggcat ccaccccatc agccgccagt accgttgtcg ctcccaatga
     7081 ggaaaaccac gaatccagaa gcgagcgaaa tacctgctca tcttcaacaa tgagaatctg
     7141 ttttccgacc aatggctgcg tcatgttctc tcccctgact ggctttactc aatagtggca
     7201 tgctattgcc acctgcgcct gtcagaatta acttaagtgt agataaaaat tctggtcaaa
     7261 tgttggtgcg taccaacggc aaaagttcat ccattttctt ttccactgcc agctgcccag
     7321 ccgcaatggc agcgtgcgcg cgatggaaat caagcgtaga tatttgcggg caaacaggtt
     7381 gaatcagaat atcgggcgga tcacctgcca tgcggttcct tttaaggcgg ttctccagca
     7441 cttggataga agtggtcata atctctgttg ccgttggcgc tgtcaccgcc cgacgtgtcg
     7501 ttatgctgcc caacctttct ttcagacgcg catgccacgg cagagaatca ccattctcgc
     7561 tattttcttc actaacatta aaggagagca gatcttgttg catcaaatga gcatcgtgct
     7621 gcaggtcgac cgctatcaca atatcagcac ccaatgcacg cgtgagcgaa ataggaattg
     7681 ggttaactac agcgccatca accagccagt agccgttatg cgcaacgggt gccattagtc
     7741 ctggaatact gcatgatgca cgaatagcaa gatggagatc gccttcagta aaccataatt
     7801 cgcgcccagt acttaaattg gtggcaacag ccgcgaagcg acgggaacaa ttttcgatct
     7861 ctgtttccgg cattatttcg cgatattgat tgaagacacg ctcgccgcgc agtaacccac
     7921 cgcgctgcca ggagagatcc atcaggcgta aaacatccca ataactgaaa gaggtcaccc
     7981 aatcttccag cgcagatagt cgatcgcatg cataggcagc gcccaccagc gaaccaattg
     8041 aacatcctgc aacgatatca atttcaatac ccactttttt tagcgcatta ataacgccaa
     8101 tatgcgacca acctctcgcc gcgccagatc ccagcgccag ccctattttt atctttctca
     8161 ttattcccgc aagattcccc tggaatgcaa tcgtagcaac atcgctactg ctcggttaac
     8221 atagtgctac tcaggcggtt aatacgccgt tattgtttcc agggagatta tttgtgtctc
     8281 agctttgtcc ctgtggtagt gctgtcgagt atagcctatg ttgccaccct tatgtgtctg
     8341 gtgaaaaggt tgcacctgat ccagaacatc tcatgcgttc gcgttactgc gcttttgtga
     8401 tgaaagacgc agattattta ataaagacct ggcatccatc ttgtggagcg gcggcgttac
     8461 gtgccgaatt gattgccgga tttgcgcata ctgaatggtt aggattgacc gtatttgaac
     8521 attgttggca ggacgtcgac aatatcggat ttgtcagttt tgttgcgcga tataccgaag
     8581 gtggcaaaac aggtgcaatc attgaacgtt cgcgattttt gaaagaaaac ggtcaatggt
     8641 actatattga cggtacacgt ccgcagtttg gccgcaacga tccctgccct tgtggttcag
     8701 gtaaaaaatt taaaaagtgc tgcggccaat aatggttgac ggtacggttt agcaaacact
     8761 ctcaacaagg tttttccagc aatgcattca ctccaacgta aagttctgcg tactatttgt
     8821 ccggaccaaa aaggtctgat cgcacgtatt accaatattt gctacaagca cgagttaaat
     8881 atcgtacaga acaatgaatt tgttgatcac cgtaccgggc gcttttttat gcgcacggaa
     8941 ctggaaggaa tttttaatga ttccaccctg ctggcggatc tcgacagcgc attgccagaa
     9001 ggctccgtac gtgagctgaa tcctgccggt cgtcgccgga tagtgattct ggtcactaaa
     9061 gaggcgcatt gcctcggcga tttgttgatg aaagccaatt acggcggcct ggatgtcgaa
     9121 atcgcggcag ttattggtaa ccacgatact ttacgttctc tggttgagcg ttttgatatt
     9181 ccgtttgagc tggtaagcca tgaagggtta agccgcaacg agcacgatca aaagatggcg
     9241 gatgccattg atgcttatca gcctgactac gtggtgctgg cgaagtatat gcgggtatta
     9301 acgccagaat ttgtggcacg cttcccgaat aagatcatca atattcacca ttcattcctg
     9361 ccagcgttta tcggcgcacg tccttatcac caggcctatg aacgtggcgt gaagattatt
     9421 ggcgcaaccg ctcactatgt gaatgacaat ctggacgaag gtccaatcat catgcaggac
     9481 gttattcatg tcgatcatac ctacacagct gaagatatga tgcgcgcagg tcgtgacgtc
     9541 gagaaaaacg tcttaagtcg cgcactctac aaagtactgg cgcagcgcgt ctttgtttac
     9601 ggtaatcgga cgattattct ttaatcgcca gcaaaaataa ctggttacct ttaatccgtt
     9661 acggatgaaa attacccaac cagttcattt ttctcaacgt aacactttac agcggcgcgt
     9721 catttgatat gatgcgcccc gcttcccgat aagggagcag gccagtaaaa gcattacccc
     9781 gtggtggggt tcccgagcgg ccaaagggag cagactgtaa atctgccgtc atcgacttcg
     9841 aaggttcgaa tccttccccc accaccatct caagcattac ctcaaaattt gaattacccc
     9901 tggtggggtt cccgagcggc caaagggagc agactgtaaa tctgccgtca tcgacttcga
     9961 aggttcgaat ccttccccca ccaccatcac tttcaaaagt ccgtgaactc tcaaacgaat
    10021 ccgcgattaa atattctgcc catgcgggga aggatgagaa gcttcgacca aggttcgact
    10081 cgagcgccag cgagagagcg tggccgcagg caacgacccg aagggcgaag cgcgcagcgc
    10141 tgagtaatcc ttcccccacc accataactt tcaaaagccc cggaattctc aaacgaatcc
    10201 gcaatcaaat attctgccca tgcggggaag gatgagaagc ttcaaccaag gttcgacttg
    10261 agcgccagcg agagagcgtt gccgcaggca acgacccgaa gggcgaagcg cgcagcgctg
    10321 agtaatcctt ctcccaccac catcactttt caaaagtccg tgaactctca aacgaatccg
    10381 caattaaata ttctgcccat gcggggaagg atgagaagct tcgaccaagg tgcgactcga
    10441 gcgccagcga gaaagcgttg ccgcaggcaa cgacccgaag ggtgaagcgc gcagcgctga
    10501 gtaatccttc ccccaccacc atcacttcct gtaaatactc aaaacctgtt actccgagct
    10561 aattctaccc aatggcaccg cgtttaccgg acctacaata tcctcccctg taggtcggat
    10621 aagcgaagcg catccgacaa ctcaccccgt accgtgccca taaaaaaacc ccgccgaagc
    10681 ggggtctatg caaactaaaa ttcgcttagt gacgagcgcg caccagctga tacttacgtg
    10741 tcagatactc caccggtacg ctccagatgt gcaccagacg tgagaacggg aacagcaaga
    10801 ataacgtcat ccccagcacc aggtgcagac ggaagataaa cgccacacca tcaaggtgtt
    10861 gagaagcgcc accgtggaag gtcactaccg actgcgccca gccaaccagt ttcatcatct
    10921 cgctaccgtc catatgctgg gcggagaacg gaatggtcag caagcccaga gcgcactgga
    10981 taaccagcag agacaggatc aggatatccg ctccggtagt cgttgcacgc acgcgtgggc
    11041 tgaacagacg acgtttcagc aacagcacgc cgccaatcag acacagcacg ccgctggcac
    11101 caccagcaaa catcgccatt ttctgtttca cttcaatcgg cagccaggct tcatacatcc
    11161 agtgcggcgt cagcatacca aagaagtgac cgacaaaaat ccccagaata ccgatatgga
    11221 acaggttcga cgccaggttc atccctttgc gatccagcat ctggctggac gccgcgcgcc
    11281 aggtgtactg cccgtagtca taacgcagcc agctaccaat caggaagacc gccccggcta
    11341 tgtacgggta gatatcaaag aagaacatat tcaggaattg cattagtgct gtcctccggt
    11401 ggtgatattc agatattgcg gcgcgacggc accggcaaag cgacgctgat gagcagtgat
    11461 tgctgaatca ccgcagcctt tgtcagcaaa gaatttaacc tgctcttctt cccagacagc
    11521 atccagcgcc tgcggcgtat cgtcgcgcgc ttcgtcggca attttttccg ccactttgtc
    11581 gctgtcgatt gcggtattcg ccagtttcag cagcagatca aacagcacgg cataacggct
    11641 ttcacgctgt tgcagacgcg cgcagagcaa tgccagaatc ggcgcgatat ctttcaaacc
    11701 ttccacggct tcgctttgcg gcagctgcgc cagatactcc agatacagcg gcaaatggtc
    11761 gggcaattcg cggctgttta actgcaaacc gtgctgctcg tactgtgcca gcaggtccac
    11821 catcgcctga ccgcgatcgc gggattcccc gtgaacgtgt tcaaacagca acagcgacgt
    11881 ggcgcgacca cgatcgaaca gttcgctgta ctgcgcctgg gcatcgagcg gatccatcgt
    11941 cgttaaatcg cgcaggaaaa tgcccagcgc atgggcatct tctttcgaca gatttttcga
    12001 cgcagcaatt gcctcaaaca tctcttgttg atgctgccat aaagcagcat ccggatattc
    12061 aaggagacgc gatacaatca cgagttcgat catgggtgcg gctccgtttt gctggttacg
    12121 tcgatggcat cgatacggcg gctgttgaac agattgaatt tggtatctga accgtggcag
    12181 ccatcaccaa aggtaaagcc gcagccattt ttctccggga aggcttcccg cgccagttca
    12241 cgatgactgc tcggcaccac aaagcgatct tcgtagttag caatcgccag ataacggtac
    12301 atctcctgtg cctgggcttc ggtcagaccg acctcttcca gcgcacgggt atcgacttta
    12361 ccgtcaacgg tttcagcacg tttgtaatga cgcatcgcca gcatacgttt cagcgcgcgc
    12421 agtaccggtt tggtatcacc ggcggtcagc agattcgcca gatactgtac cggaatacgc
    12481 aggctttcga cgtctggcag aatgccgttg ctgcccagct cacccgcgtc tgctgcagac
    12541 tgaatcggag acagaggcgg cacgtaccag accatcggca gtgtgcgata ttctggatgc
    12601 agcggcagcg ccagtttcca ttccatcgcc attttataaa ccggcgactg ctgtgcggct
    12661 tcaataacgc tcagcggaat accgtcttta attgcctgct cgatgacttt cggatcgttc
    12721 ggatcgagga acacctccag ctgacgctgg tagagatctt tctcgttctc ggtgctggct
    12781 gcacgttcaa tggcgtcggc atcgtacaac agcacgccga gataacggat acgaccgaca
    12841 caggtttcag agcacacggt cggctgaccc gcttcaatac gcggatagca gaagatgcac
    12901 ttctcagact taccgctctt ccagttgaag tagatttttt tgtatgggca tccggtgatg
    12961 cacatacgcc agccacggca tttatcctgg tcgatcagga cgatgccatc ttcttcacgc
    13021 ttgtaaatcg caccgctcgg gcaggtcgcc acacatgccg ggttcaggca gtgttcgcac
    13081 aggcgcggca aatacatcat gaaggtgttt tcgaactggc tatacatcgc cttctggatg
    13141 ttgtcgaagt tcttgtcttt cgccagcttg tcaaactcac cgcccaggtc atcttcccag
    13201 ttcggccctt tttcgatttt cgccatccgt tccccggtaa tcagcgaacg cggacgggca
    13261 atcggctgcg atttgctgcc ttccggcgcg gtatgcaggt tctgataatc aaaatcgaac
    13321 ggctcgtaat aatcgtcgat ccccggcaga tgcgggttag cgaagatttt acccagcagc
    13381 atggcgcggt tacccatgcg cggttgcagt ttgccattga ttttacggat ccagccgcct
    13441 ttgtattttt cctggttttc ccagtcagtc gggaagccct ggcccggctt ggtttccacg
    13501 ttgttgaacc acgcgtattc cacgccttcg cggctggtcc agacgttttt acaggtaact
    13561 gaacaggtgt ggcagccgat gcacttatcg agattcagca ccatgccgac ttgtgaacga
    13621 attttcattt tacgctctcc tgtacctggt cattgccttc gccatctaac cagtcaatgt
    13681 tcttcatctt acgcactaca acaaactcat cgcggttaga acctacggtg ccatagtagt
    13741 taaagccgta tgccagatgg gcatagccgc cgatcatatg cgtcggtttc ggcgtgatac
    13801 gggtgaccga attatggata ccaccacgct gttgggtaat ttccgaacca ggcaggttaa
    13861 cgatacgttc ctgcgcgtgg tacatcatgg tcatccctgc cggaacacgc tggctgacaa
    13921 ccgcacgggc agtcagtgca ccgttgctgt tgaagacttc aatccagtcg ttatcggcga
    13981 tacccagctc tttggcatcg gcttcactca gccagaccac cggaccaccg cgacctaaag
    14041 tcagcatcag caggttgtcg ctgtaggtgg agtggatacc ccacttctgg tgcggcgtca
    14101 ggaagttgag cgctttttcc gggttgccgt tggatttctg gcccatcact tctttcaccg
    14161 aacgggtgtc gatcggcgga cgataaacca gcaggctttc accgaaatca cgcatccact
    14221 ggtgatcctg atacagctgc tgacggccag agagcgtacg ccacgggatc agctcgtgaa
    14281 cgttggtgta accggcgttg taagaaacgt gttcatcttc aaggccagac caggtcgggc
    14341 tggagatgat tttgcgcggc tgtgcctgaa tatcgcggaa gcgaatcttc tcgtcttctt
    14401 tattcagcgc cagatgcgta tggtcacgac cggtaaactc gctcagggca gcccaggctt
    14461 ttacagctac ctgaccattg gtttccggtg ccagcgtcag gatcatctct gccgcatcaa
    14521 ttgcggtatt gagcatcggc tgaccttttg ccggaccttc cgctttggtg tagttgagct
    14581 tacgcagcag atccatctcg ctctgggtgt tccaggcaat ccctttaccg ccgttaccga
    14641 ttttctccat cagcgggccg atagaggtaa agcgttcgaa agttgccgga taatcgcgct
    14701 ctacgaccat aatgtgcggt gcagttttac ctgggatcag atcgcattcg ccttttttcc
    14761 agtctttcac atccagcggt tgcgccagtt cagcggcaga gtcgtgctgg ataggcagcg
    14821 tgacgatgtc agtttcttta cccagatgac caacgcacac ttcggagaat ttcttcgcga
    14881 tggctttgta gatttcccag tcgcttttcg cttcccaggc cggatcgacc gccgcagaca
    14941 gcgggtgaat aaacggatgc atatccgaag tattcatgtc gtctttttcg taccaggtcg
    15001 ccgtcggcaa aatgatgtcg gaatagagac aggtgctcga cagacggaag tccagcgtca
    15061 ccaccagatc cagcttgcct tccagaccgt tgtcctgcca gtccacttct tccggcttca
    15121 cgccgccctg ctgccccagg tctttgccct ggataccgtg ctccgtcccc agcaggtact
    15181 tgagcataaa ctcatgacct ttaccggaag aaccgagcag gttagaacgc cagatgaaca
    15241 ggttacgcgg gtggttttta ccgttttctg gttgttctgc cgcaaaacgg atagaaccct
    15301 ctttcaggga tttcacggtg taatccaccg gattcatgcc cgcttttttc gcttcgccag
    15361 cgatagttag cgggttagtg cctaactgcg gcgcagacgg cagccagccc atacgttctg
    15421 cacggacgtt aaagtcgatc aagtgtccgg tataacggga tttatccgcc atcggcgaca
    15481 acaactcttc tgcggtcacg gtttcataac gccactggct ggagtggtta tagaaataag
    15541 aggtgctgtt catgtgacgc gccggacgct gccagtcaag agcaaacgcc agcggctgcc
    15601 agccggtttg cggacgcagt ttttcctgac ctacatagtg cgcccagcca cccccgctct
    15661 gaccgacaca gccgcagaaa atcagcatgt tgatcagacc acgatagttc atatcgaggt
    15721 gataccagtg gttcagcccc gcaccgacga taatcatcga acgaccgtgc gtcttgtcag
    15781 cgttatcggc aaattcacgg gcaatacgga taatctggct acgagaaacg ccagtgattt
    15841 gctcggccca cgccggagtg taagctttca catcgtcata gctggttgcg cagttaacgt
    15901 cgttcaggcc acgttccaga ccgtagtttg ccagtgtcag atcataaacg gtggtcacca
    15961 gtgcggtgct gccatcagcc agttgcaggc gttttaccgg cagtttgtgc agcagaatgt
    16021 tttccagctc cactttgttg aagtgttccg tgccgtcgcc accaaagtac gggaagccca
    16081 cctcagcgat ctcatcctgg ctacccagca gactcagttg cagctcggtt tcttcgccag
    16141 ttttgccgtc gcgctgctca agattccatt tgcccttctc gccccagcgg aagccaatag
    16201 aaccgttcgg cgcaaccatt tcgccattgg tattaaaggc gacagttttc cattccggat
    16261 tgttttcctg gcccagcgca tccaccagat cagcagcacg cagcatacga cctgcggcgt
    16321 agtaaccgtc gcgttcttcc agcatcacca gcatcggcat gtcggtgtag cgacgcacat
    16381 agtcggtgaa atactggctt gggttgtcga ggtggaattc acgcagcatt acgtggccca
    16441 tcgccagcgc cattgctgcg tcggtgccct gtttcggtgc cagccacaga tcgcacagtt
    16501 tggcgatttc agcgtagtcc ggtgtgacgg caacagtttt ggtgcctttg taacgcactt
    16561 cagtaaagaa gtgagcatcc ggagtacgcg tctgcggcac gtttgaaccc caggcgatga
    16621 tgtagctgga gttgtaccag tcagcagatt ccggtacgtc agtctgctcg ccccaggttt
    16681 gcggagacgc aggaggcagg tcgcagtacc agtcgtagaa gcttaagcaa gtaccgccaa
    16741 gcagcgagag ataacgcgca cccgacgcgt aagaaaccat cgacattgcc ggaattggcg
    16801 agaagccagc gacacggtcc gggccgtagt ttttgatggt gtaaacgtta gatgcggcga
    16861 tcagttcgtt cacttcctgc caggaagaac gaacaaaacc accgcgtcca cgcgcctgct
    16921 taaagctttt cgctttatcg gcgtcttcaa tgatagaagc ccatgcctca atcggatcgc
    16981 tatgcagcgc cttcgcttca cgccacattt tcatcaggcg tttgcgcatc atcgggtatt
    17041 tcaggcggtt ggcactgtaa agataccagg agtagctggc accgcgcggg cagccgcgag
    17101 gttcatggtt tggcagatcc ggacgggtac gaggatagtc agtctgctgg gtttcccagg
    17161 tcaccagacc gtttttgacg tagattttcc agctgcagga gccggtgcag tttaccccgt
    17221 gggtagagcg gacgattttg tcatgctgcc aacgctggcg atatccatcc tcccagtcac
    17281 ggttggtatt gagaagctgg ccatgcccat cggcaaaggt ttcacccttc tgtttgaagt
    17341 agcgaaaccg gtccaggaat ttactcatcg gttttctcct gtgggagcct gtcggccctc
    17401 tgataaatcg acattgctaa tgtttaaggg caaggtaacg ctctgaaaca gcgtgagaat
    17461 tgataacgat caagagtgat ggggatgaaa aataaagtaa attcctaaga taccccttta
    17521 ggataattaa cagccacaac ataacagact gtaaattaag acgttttata cctgtagtta
    17581 tttaaactat taagattttt atgcagatgg gtattaagga gtattcccca tgggtaacat
    17641 attaatggct cttaatgaaa tccggcgaaa taagccactc acacatgagt atgttgtaac
    17701 taaacgacag ataaaaaaag cgcgacatca tgccgcgcaa aggataatca aatattaaga
    17761 tttatttttt agaatgccga ccatataccg cccaggtaat cactacacag gcgatataga
    17821 aaatcaaaaa tactttcatt gcgccgactg gcgaacccgt taatgccagc gagctaccga
    17881 acgctttcgg gataaagaag ccaccaatcg cgccaatcgc agagatgaaa cccagcgccg
    17941 ccgccgtgtc ggttgccgct tcacgcatcg cacgttcgtc agaaccccct tctgctttca
    18001 cgcgatccat tgtcagttta cggaagatca cggaaatcat ctggaaagtg gaaccactac
    18061 ccagcccagc tgtcaggaac agcgccagga agaccgcgaa gaacgccatg aagcttccgc
    18121 cctgcccgtc agtcggtaag gtcaggaaca gcaggccgct gaaaatcgcc atcaggataa
    18181 agttcaccag tgtgacacga gttccgccca gacggtcaga taatgcacca cctgcagaac
    18241 gcgccagcgc accaataaac ggcccgaaga aagcgtattg cagaatctga acatccggga
    18301 actgcgtttt tgacagcatc gcaaagcccg cggagaagcc gatgaaggag ccgaaggttg
    18361 ccagatacag caggctcata atccacagat gaccccgttt gagtaccggc aactgctcct
    18421 tgatggaggc tttcgaggta gcaagatcgt tcatgccaaa ccacgccgca atggtgaaga
    18481 tggcaaggaa cggcacccat acccaggacg cattcgccag atacagctca gtcccatccg
    18541 gctgtttgac accctggcta ccaaatactg cgaaaatcga cagtgatacc accagcggag
    18601 caaccaactg catgacgctg acgcccatgt tgcccagacc accattcaga cccagcgcgc
    18661 caccctgctt ctgtttcgga aagaagaagc tgatgtttgc catactggaa gcgaagttcg
    18721 cgccagcaaa gccgcacagc agagagatga tgatgaagac gctataaggc gtggaggtat
    18781 cctgcacggc aaaacccagc cagacgcaag gaataatcag aataccggtg ctgaacgccg
    18841 tccagcgacg accaccgaag ataggaacca taaaggagta tggaacacgt aataacgcgc
    18901 cagaaaccga aggcagcgca gtcaacataa atagctgatc ggtcgtaaaa ttaaaaccaa
    18961 cttttggtaa gttcaccgca acagcgctga acaacatcca tacgcaaaac gccagcagca
    19021 gacagggaac ggaaatccac aggttgcggc tggcaatacg ttgaccgcgt tgttgccaga
    19081 acgcaggatc ttccggtcgc caatctgtaa tgacagctcc agtagccctt tcgggggcgg
    19141 atgagtgact catagacacc tctgatactc gtttcgatgt ctgccacctt agtgtctgta
    19201 gctaaaggca atttgatgta aatcaaacga taagataact ttatcta
//
LOCUS       DACBVI010000084        19204 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.084, whole genome shotgun sequence.
ACCESSION   DACBVI010000084 DACBVI010000000
VERSION     DACBVI010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.084"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            4..2394
                     /locus_tag="IU325_14285"
     CDS             4..2394
                     /locus_tag="IU325_14285"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound PQQ-dependent dehydrogenase,
                     glucose/quinate/shikimate family"
                     /protein_id="HAP5810173.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(2441..3991)
                     /gene="cueO"
                     /locus_tag="IU325_14290"
     CDS             complement(2441..3991)
                     /gene="cueO"
                     /locus_tag="IU325_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HAP5810174.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNNPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPATHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            4157..4504
                     /locus_tag="IU325_14295"
     CDS             4157..4504
                     /locus_tag="IU325_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HAP5810175.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKTLGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            4610..5476
                     /gene="speE"
                     /locus_tag="IU325_14300"
     CDS             4610..5476
                     /gene="speE"
                     /locus_tag="IU325_14300"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HAP5810176.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDASVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTNQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            5492..6286
                     /gene="speD"
                     /locus_tag="IU325_14305"
     CDS             5492..6286
                     /gene="speD"
                     /locus_tag="IU325_14305"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HAP5810177.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(6324..6686)
                     /locus_tag="IU325_14310"
     CDS             complement(6324..6686)
                     /locus_tag="IU325_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0231 family protein"
                     /protein_id="HAP5810178.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVED
                     AARTLKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(6883..9480)
                     /gene="acnB"
                     /locus_tag="IU325_14315"
     CDS             complement(6883..9480)
                     /gene="acnB"
                     /locus_tag="IU325_14315"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HAP5810179.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            9835..11688
                     /locus_tag="IU325_14320"
     CDS             9835..11688
                     /locus_tag="IU325_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HAP5810180.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEAQSTQ
                     LTVSDAAAVTLPATVSAPPVTPAVVKSAFNTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKTVPVTQTVTAPVIPSN
                     TVLTANHVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNDWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRDGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARERIQSASPEQRQ
                     AVREKMQTNPQNQQRREAARERIQSATPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFREKVQESRPQRLNDSDHTVRLNNEQRSAVRERLSERGARRLER"
     gene            complement(11759..13183)
                     /gene="lpdA"
                     /locus_tag="IU325_14325"
     CDS             complement(11759..13183)
                     /gene="lpdA"
                     /locus_tag="IU325_14325"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP5810181.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(13508..15400)
                     /gene="aceF"
                     /locus_tag="IU325_14330"
     CDS             complement(13508..15400)
                     /gene="aceF"
                     /locus_tag="IU325_14330"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP5810182.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAGPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAVKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAVTGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(15415..18078)
                     /gene="aceE"
                     /locus_tag="IU325_14335"
     CDS             complement(15415..18078)
                     /gene="aceE"
                     /locus_tag="IU325_14335"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HAP5810183.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(18239..19003)
                     /gene="pdhR"
                     /locus_tag="IU325_14340"
     CDS             complement(18239..19003)
                     /gene="pdhR"
                     /locus_tag="IU325_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HAP5810184.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         4419 a         5325 c         4834 g         4626 t
ORIGIN      
        1 tttatggcaa ttaacaatac aggctcgcga cgattactcg tcacgctaac agcccttttt
       61 gcagcgcttt gcgggctgta tctactcatt ggcggaggct ggctggtcgc gattggcggc
      121 tcctggtact accctatcgc tggccttgtg atgctcggcg tcgcctggat gctgtggcgc
      181 agtaaacgcg ccgcgctttg gctatacgcc gccctgctgc tcggcaccat gatttggggc
      241 gtctgggaag ttggtttcga cttctgggcg ctgactccgc gcagcgacat tctggtcttc
      301 ttcggcatct ggctgatcct gccgtttgtc tggcgtcgcc tggtcattcc tgccagcggc
      361 gcagttgccg ctctggtggt ggcgttgctg attagtggcg gaatcctcac ctgggcggga
      421 tttaacgatc cgcaggaaat caacggcacc ttaagcgccg atgccacacc tgccgaagcc
      481 atctcaccgg ttgccgatca ggactggcct gcgtatggtc gcaatcagga aggtcaacgc
      541 ttttcgccgc tgaaacagat taacgccgat aacgtccata acctgaaaga agcctgggtg
      601 ttccgtactg gagatgtgaa gcagccgaac gatccgggcg aaatcaccaa tgaagtgacg
      661 ccgattaaag tgggcgacac cctttacctg tgtaccgctc accagcgcct gtttgcgctc
      721 gatgccgcca gcggcaaaga gaaatggcat tacgatcctg aactgaaaac caacgagtct
      781 ttccagcacg taacctgccg tggcgtctct tatcatgaag ccaaagcaga aacggcctcg
      841 ccagaagtga tggcggattg cccgcgtcgt atcattcttc cggtcaatga tggtcgcctg
      901 attgcgatta acgctgaaaa cggcaagctg tgcgaaacct tcgctaataa aggcgtgctc
      961 aatctgcaaa gcaatatgcc agacaccaaa ccgggtctgt atgagccgac ttcgccgccg
     1021 attatcaccg ataaaaccat cgtgatggcc ggttcagtca ccgataactt ctcaacccgc
     1081 gaaacgtctg gcgtgatccg tggttttgat gtcaacaccg gggagctgct gtgggctttt
     1141 gatccgggcg cgaaagatcc gaacgcaatc ccgtctgacg aacacacctt tacctttaac
     1201 tcaccaaact cgtgggcacc agcggcctat gacgcgaagc tggatctggt ctatctgccg
     1261 atgggcgtga ccacgccgga tatctggggc ggtaaccgca caccggaaca ggaacgttat
     1321 gccagctcga ttctggcgct gaatgccaca accgggaaac tggcgtggag ctaccagacc
     1381 gttcaccacg acctgtggga tatggatctt ccggcacagc cgacgctggc ggacatcacc
     1441 gttaacggtc aaaaggttcc ggtcatttat gctccagcga aaaccggcaa catttttgtg
     1501 ctcgatcgtc gtaacggcga actggtggtt ccggccccgg aaaaaccggt tcctcaaggt
     1561 gcagcgaaag gcgattacgt caccccaact caacccttct cggaactgag cttccgtccg
     1621 acgaaagatt tgagcggtgc ggatatgtgg ggagccacca tgtttgacca gctggtgtgc
     1681 cgcgtgatgt tccaccagat gcgctatgaa ggcattttca ccccaccatc tgaacagggt
     1741 actctggtct tccctggtaa cctggggatg ttcgaatggg gcgggatttc cgttgatccg
     1801 aatcgtgaag tggcaattgc caacccaatg gcactgccgt ttgtttcgaa actgatcccg
     1861 cgcggtccgg gtaatccgat ggaacagccg aaagatgcca aaggcacggg tacggaatcc
     1921 ggcattcagc cgcagtacgg tgtaccgtat ggcgtcacgc tcaatccgtt cctctcaccg
     1981 tttggtctgc catgtaaaca gccagcatgg ggctatatct cggcgctgga tctgaaaacc
     2041 aatgaagtgg tgtggaagaa acgtattggt acgccgcagg acagtatgcc gttcccgatg
     2101 cctgtaccgg tgccgttcaa tatgggtatg ccgatgctgg gcgggccaat ctccacagcg
     2161 ggtaacgtac tgtttatcgc cgctacggca gataactacc tgcgcgctta caacatgagc
     2221 aacggtgaaa aactgtggca gggccgttta ccggcgggtg gtcaggctac gccaatgacc
     2281 tatgaagtga atggtaagca gtatgtggtg atctccgcag gcggtcacgg ttcatttggt
     2341 acgaagatgg gcgactatat tgtggcttat gcgctgccgg atgatgtgaa gtaaccataa
     2401 tgcccggaga gatccgggca tatttccgaa tacggtcttt ttataccgta aaccctaaca
     2461 tcatccccgt atcttcatgc tccagcaaat ggcagtgcgc catataagca tgttctttcg
     2521 gtgcatcgtg attaaacttc accagcactt cgctgacatt accttctacc ttaacggtat
     2581 ctttccagcc tgcgcgatga gtcgctggag gtttgccatt ttctgacaag atacggaact
     2641 gcgtgccgtg aatatggaac ggatgcagca tcatgtcgcc cacgccagag ataacccaac
     2701 gttcgtactg ccctttcgcc gccgcaaaca tcggcttatt catatcaaac gcctgaccgt
     2761 tgattttatt ggcatggtgg aaatcgaact tcccgccgtg gttcatatga ttcatattgc
     2821 cgtgccccat atggcccatc atctggctgt gatccatccc ggccatcgcc tgatcgccat
     2881 atttctccat cagcatctgc atccccatca tatcgagcat cgggtccata gagagttgca
     2941 gcttacgtac cgtcagccct tccagcgaag gtaatgctgg caggctactt aatgtgtctg
     3001 gcaaagcacc agaggcacta atggcaatcg gctgaatccg cattaccgga tgcggcttat
     3061 caaaaggcgc aatcgccatt cccatctggc tgaccggcag cgtcaccagg tcaaaggggt
     3121 tgttgtcgtt aacctccacc agcacttcaa aacgctcgcc catcagcacc ggcagctcgc
     3181 tcaccttcac cggttcaggt agtagaccac cgtcgctggc aatcacatac agcgggcgat
     3241 tgtcgctggt ggcgaaattg agcgagcggg cgttacagcc attgagcaaa cgcaggcgca
     3301 gccaaccacg cggggcagcg tgttgtgggt agattgcacc gttggtcagc aacgtatcgc
     3361 caaaccagcc cacggcggcg gtcatcacat ccagttgata atcaatctgc ccgtcagcgc
     3421 taaatttctt atcctgaacg atcaccggaa catcatcgat accccactgt tttggcagca
     3481 ttaatttcag gatctcgtca tcttcaatca ccaccagacc agccagcccc atcgccacct
     3541 gtcgcccggt cttgccatgt tgatgtggat ggaaccagca ggtagcggca ggttgatcaa
     3601 cgttcaacgt caccgagcgc ttgccacctg gcggaataat tccctgcggg ccgccgtcga
     3661 cttcacccgg tacttccagc ccgtgccagt gcaacgtcgt ctcttccgtc agttggttat
     3721 agatatcaac cgttaccgct ttgccgcgct gtaatttcac cgccggcccc agcagattgc
     3781 cgttatagcc ccaggtagtt gcggttttcc cgccaaaggt ggactggcct gcgccaatag
     3841 ttaactgaat gcgattacgg gcatcggtcg tgagcaaatc agggatcggt aacgttgggc
     3901 gttccgccgc aaatactgcg cggctccaca gcggtaaagc cgaagccacg cccagcgcga
     3961 cggaatattt taagaaatca cgacgttgca tagttatttc cttattctta agcaggcgaa
     4021 acaaaatcaa acgttgagca tagtccttcc ccttacggga aggtcaagcc gcagacacaa
     4081 tttaatcgac aataatggtc gtcgcctcgc tcgcagtgtg ctaacgttta tcttctttaa
     4141 gcccctggta gaagcaatga agacgttttt cagaacagtg ttattcggca gcctgatggc
     4201 cgtctgcgca aacagttacg cgctcagcga gtctgaagcc gaagatatgg ccgatttaac
     4261 ggcagttttt gtctttctga agaacgattg tggttaccag aacttaccta acgggcaaat
     4321 tcgtcgcgca ctggtctttt tcgcccagca aaaccagtgg gatctcagta attacgacac
     4381 cttcgacatg aaaaccctcg gtgaagacag ctaccgcgat ctcagcggca ttggcattcc
     4441 cgtcgctaaa aagtgcaaag ccctggctcg cgattcctta agcctgcttg cctacgtcaa
     4501 ataatccctg ataccttttt gtagaaatat tgaccgtgca tccgcggtca atgttagcta
     4561 ttatgttgcg cccttttttt acgggtgtta acaaaggagg tatcaaccca tggccgaaaa
     4621 aaaacagtgg catgaaacgc tacacgacca gtttgggcag tactttgcgg tagataacgt
     4681 tctgtatcat gaaaagaccg atcaccagga tctgatcatt tttgagaacg ctgcatttgg
     4741 ccgcgtaatg gcgctggatg gcgtagtaca aaccaccgag cgcgacgagt ttatctatca
     4801 tgagatgatg acccatgttc cgctactggc ccacggtcac gcgaaacatg tgctgattat
     4861 cggcggcggc gacggtgcca tgctgcgtga agtgacccga cataaaaacg ttgagtcaat
     4921 cacgatggtg gaaatcgatg cgagtgtcgt gtcgttctgc cgtcagtatc tacccaacca
     4981 taacgctggt agctacgacg atccgcgctt taaactggtg atcgacgatg gcgtcaattt
     5041 cgttaatcaa accaaccaga cttttgatgt cattatctcc gactgcaccg atcctatcgg
     5101 tcccggcgaa agtctgttca cctcagcatt ttatgaaggt tgtaaacgct gcctgaatcc
     5161 tggcggtatc ttcgtcgcac aaaacggcgt ctgcttttta cagcaggaag aggctatcga
     5221 cagtcatcgc aaactcagcc attacttcag cgacgttggc ttttatcagg cggcgatccc
     5281 gacctattac ggcggcatca tgacctttgc atgggcgaca gataacgatg ccttacgcca
     5341 tctctcaacc gaaattattc aggcgcgttt tctcgcttct ggcctgaaat gccgttatta
     5401 caatccggca atccatacgg cagcttttgc cttacctcag tatctgcaag acgcactggc
     5461 ttcacagccg tcctaaggag aagataagaa attgaaaaaa ctgaaactgc atggctttaa
     5521 taatctgacc aaaagtctga gtttttgtat ttacgatatc tgctacgcca aaactgccga
     5581 agaacgcgac ggttatattg cttatatcga tgaactctat aatgccaacc gtctgaccga
     5641 aatcttgtca gaaacctgtt ccattatcgg ggccaatatt cttaacatcg cccgccagga
     5701 ttacgaacca cagggtgcca gcgtcactat tctggtgagt gaagaaccgg ttgacccgaa
     5761 actcatcgac aaaacagaac accccggccc actgccagaa acggtcgttg cccatctcga
     5821 taaaagtcat atttgcgtac atacctaccc ggaaagtcat cctgaaggcg gtttatgtac
     5881 cttccgcgcc gatattgaag tctctacctg cggcgtgatt tctccgctga aggcgctgaa
     5941 ttacctgatc caccagcttg agtccgatat cgtaaccatt gattatcgcg tgcgcggttt
     6001 tactcgcgac attaacggta tgaagcactt tatcgaccat gagattaatt cgattcagaa
     6061 ctttatgtct gacgatatga aggcgctgta tgacatggtg gatgtaaacg tctatcagga
     6121 aaatatcttc cataccaaga tgttgcttaa agagttcgac cttaagcact atatgttcca
     6181 caccaaaccg gaagatttaa ccgacagcga gcgccaggaa attaccgctg cgctgtggaa
     6241 agaaatgcgc gagatttatt acgggcgcaa tatgcccgct gtttaacggc tctggcggag
     6301 ctcgcgggct ccgccagatt tatttacttt tgctgcacga aattacgata agcagccacc
     6361 acctgcagaa aatcctcaac gccgcatagc gacaggcttt cttcgtcgta gtagttcatc
     6421 ccctcttcca tttcatcgcc agcgaattcc agttgattgg cgcgaaccat cacttcttcg
     6481 ccgtccatcc atagcgtgta ctcatgccct gcccgctgcc aggaacgttc gctacctttg
     6541 agcgtgcgcg cggcgtcttc tacttcatca agcaaggcca ggttttcttt cacctcttcg
     6601 ttaaaccagt gcccgaccac ttcatgcccc atggacatac gcacctttac cactccggta
     6661 atatcacgca gaaattcgta atccatagtg tgttcctctg ctcccggcaa tgcgccatta
     6721 ctgcattgca ccttaggttt aattatcgca gcactatgca agattaaaag gtggaaaaga
     6781 ataaggaata aaaataaacc ccctttcctt gagcagaaag ggggtttaaa gaagtgttac
     6841 ttgtcaggta ttcaggagaa cacctgagct attttcaggc tgttacaccg cagtctggaa
     6901 aatcaccccg tccgcttttt cggtgtactg agaaagctgg ttgaagttca ggtaacggta
     6961 agtatcaacg gcagttttat ccacctgtgc cacgtaggtc tggtactctt ccggcgtcgg
     7021 cagtttgcca atcagcgccg caacagcagc cagttccgca gaagccagga agacattcgc
     7081 gccagtaccc aaacggttcg ggaagttacg ggtagaggtg gaaaccaccg tcgcaccgtc
     7141 tgctacacgc gcctggttac ccatacacag ggaacagcca gggatctcga tacgcgcacc
     7201 actcttaccg aagacgctgt agtagccttc ttccgtcagt tgcgcggcgt ccatacgggt
     7261 tggcggtgcc acccacaggc gggtcggcaa ctggccttta tgcgcatcca gcagtttacc
     7321 cgcagcacgg aagtgaccga tgttggtcat gcaggaaccg ataaacactt cgtcgatctt
     7381 ctcaccctgt accgcagaca gcggacgtgc gtcatccgga tcgttcggag cacacaggat
     7441 tggctcttta atatccgcca gatcgatgtc gatcactgcc gcgtattccg catctgcatc
     7501 ggcttccagc agctcaggat tcgccagcca tttttccatg ccctgaatac ggcgttccag
     7561 ggtacgacga tcgccgtaac cttcggcaat catccacttc agcaggacga tgttagagtt
     7621 caggtattca atgatcggtt ctttgttcag cttaatggta caaccggcag cagaacgctc
     7681 ggcggacgca tcggtcagct caaaggcctg ctcaactttc agatccggca gaccttcaat
     7741 ttccagaatg cgacctgaga agatgttttt cttgcctttc ttctcaacgg tcagcagacc
     7801 ttgtttgatc gcatacagcg gaatagcgtg taccagatcg cgcagggtga tgcccggctg
     7861 cattttgcct ttgaagcgca ccagaacgga ttccggcata tccagcggca tgacgcctgt
     7921 tgcggctgca aatgccacca gaccagaacc cgccgggaaa gagataccga tcgggaaacg
     7981 ggtatgggag tcaccaccgg taccgacggt atccggcagc agcatacggt tcagccagga
     8041 gtggattacg ccgtcacccg gacgcagcga cacaccacca cggttcataa tgaagtccgg
     8101 cagcgtgtgg tgcgtgttca cgtcaactgg cttcggatac gccgcggtgt ggcagaaaga
     8161 ctgcatcacc aggtcagccg agaagcccag gcacgccagg tctttcagtt catcacgggt
     8221 catcgggccg gtggtgtcct gtgaacctac agaagtcatt ttcggttcgc agtacgcgcc
     8281 cggacgaatg cctttcacgc cgcaggcacg acctaccatt ttctgcgcca gcgagaagcc
     8341 gcggtcgctc tcagcgacat ctttcgcctg acggaacaca tcgctgtgcg gcagaccaag
     8401 tgcttcacgc gctttggtgg tcaggccacg cccgataatc agcggaatac gaccaccggc
     8461 acgcacttca tcaatcagca cgtcggtttt cagctcgaag gtcgccagca gttcgccggt
     8521 ttcgtggtta cgcacttcac ctttgtacgg gtaaacgtca attacgtcac ccatgttcag
     8581 gttagagacg tccacttcaa ttggcagtgc acccgcatct tccatcgtgt taaagaagat
     8641 gggtgcaatt ttaccgccga ggcacaaacc accgccgcgt ttgttcggca catgtggaat
     8701 atcatcgccc ataaaccaca gaacggagtt cgtggcggat ttacgcgatg aacccgtacc
     8761 cacaacgtca ccgacgtacg ccagcggaaa acctttctgt tgcagagctt caatctgttt
     8821 gatcggacca acaacacctg gctggtctgg ctcaatacct tcacgggcgt ttttcagcat
     8881 cgccagcgcg tgcagtggga tatccgggcg tgaccacgca tccggtgccg gagagaggtc
     8941 atcggtgttg gtttcgccag tgactttaaa gacggtaacg gtcagttttt cagccagcgc
     9001 cgggcgattc aggaaccatt cggcatccgc ccaggactgc attacctgct tagcatattc
     9061 attgcctgct ttggctttct cttctacgtc atagaagtta tcgaacatca gcagtgtgtg
     9121 agaaagtgct ttggcagcaa tcggtgccag tttggcatca tccagcgcgt cgatcagcgg
     9181 atgaatgttg taaccaccct gcatggtgcc cagcagttcg atagcttttt ccggagtcag
     9241 cagaggggat ttggcttcgc ctttagcaat agcagccagg aagcctgctt ttacataggc
     9301 ggcttcatcg acgcctgggg gaacacggtt ggttaacaga tctaacagga attcttcttc
     9361 gcccgcgggc gggtttttca gcagctctac aagcgcggcc atttggtttg catccagggg
     9421 tttgggcgca atcccctcag cggcacgctc agctacgtgc ttacggtatt cttctagcac
     9481 gacggttctc ctcgctctca ttgtcatagt gcggcaggcg attctcttca cgctcctgtg
     9541 agacagcagt ttgtagggta aatgcccagt accgcaacgg gcagaatagc aggattttga
     9601 cgatgtgtta atccgtttac aaaaaagcaa cataaaaaag tggctgaatc gttaaggatg
     9661 gtctagagat tgtttccctc cgtaaaaact tcgttaattc gcatggtgat aatcaccttt
     9721 ctcaacgaat cccaagatta gtcaaaattt aaactaccgc ctctttatac tcggattctc
     9781 agcacctgcg ggtggcagtt cgccgaccat tgcgatttcc ttgagatccg aattatgaaa
     9841 atgactttgc cgtttaaacc ccatgtgctg gcgctaattt gcagtgccgg gctttgtgcc
     9901 gcctctgccg ggctatatat aaaaagccgc acagtggaag cgcctgtgga agcgcaatcg
     9961 acacaactga ctgtgtctga cgctgccgcg gttacgcttc ctgcaacggt ttccgcgcct
    10021 cccgtaacac ccgccgtcgt caaatccgca ttcaacactg cacaaataga tcaatgggtt
    10081 gcgcctgtcg cgctgtaccc cgactctctg ctttcacaag tgttaatggc atcaacctat
    10141 ccgacaaacg ttgcgcaagc agtgcaatgg tcgcacgata atccacttaa acaaggcgat
    10201 gctgctattc aggcggtatc tgaccagccg tgggatgcaa gcgtcaaatc actggtagcc
    10261 tttccacagt tgatggcgtt gatgggcgaa aacccgcaat gggtgcaaaa cctgggcgat
    10321 gcttttctgg cgcagccgca ggacgtgatg gactcggtac agcgattgcg gcaactggcg
    10381 caacaaaccg gctcgctgaa gtcatcaacc gaacagaaag ttattaccac aacgaagaaa
    10441 actgtaccgg taacacagac agtcacggct cccgtcatac catccaatac cgttttaact
    10501 gccaaccacg tcattacaga gccggcaaca accgtcattt ctattgagcc cgccaatcct
    10561 gatgtggtct atattcccaa ctacaaccca accgtggttt acgggaactg ggccaatact
    10621 gcgtatccgc cggtttatct gccaccacca gccggagaac cgtttgttga cagctttgtg
    10681 cgcggattcg gctatagcat gggcgttgct accacgtacg cgctattcag cagcatcgac
    10741 tgggatgacg acgatcatga ccatcatcat catgacgatg atgattatca tcaccacgat
    10801 ggcggtcatc gtgacggtaa tgactggcaa cacaacggcg acaacatcaa tatcgacgtc
    10861 aataatttca accgtatcac cggtgagcat cttactgata agaatatggc atggcggcac
    10921 aatccaaact accgtgatgg tgtgccctat catgatcagg atatggcaaa gcggtttcat
    10981 caaaccgatg tcaacggcgg catgagcgcc acgcagttac ctgctccaac acgcgacagc
    11041 cagcgtcagg cggcagcaag tcagtttcag caacgaacac acgccgcccc cgtcattacg
    11101 cgagataccc aacgtcaggc agcggcacag cggtttaatg aagctgaaca ctatgggagc
    11161 tatgacgact tccgcgactt cagccgtcgc caaccactga cccagcaaca aaaggacgcc
    11221 gctcgtcagc gttatcagtc ggcctctcct gagcagcgcc aggcagtccg cgagaaaatg
    11281 cagactaacc cgcagatcca gcagcgaaga gaggcagcgc gtgagcgtat tcagtccgcc
    11341 tctcctgagc agcgccaggc agtccgcgag aaaatgcaga ctaacccgca gaaccagcag
    11401 cgaagagagg cagcgcgtga gcgtattcag tctgcaactc ccgaacagcg ccaggtgttt
    11461 aaggaaaaag tacagcagcg cccactgaac caacagcaac gtgataacgc ccgccagcgt
    11521 gttcaatcag catcacctga gcaacgtcag gtttttcggg agaaagttca ggagagccgc
    11581 ccacaacgtc taaacgacag tgaccatact gtcaggctga ataacgagca acggtcagca
    11641 gtacgcgaac gtctctctga gcgcggagca aggcgactgg aaaggtaaat tacagacgta
    11701 aaaaaagcgg cgtggttagc cgctttttta attgccatat gtcccggcaa acgaagaatt
    11761 acttcttctt cgctttcggg ttcggcaggt cggtaatgct accttcgaac acttctgccg
    11821 ccaggcccac agactcgtgc agagtcgggt gcgcatggat ggtcagtgcg atgtcttcag
    11881 catcacaacc catttcgatt gccaggccga tttcacccag cagctcgccg ccgttagtac
    11941 cgacaatcgc accaccgatc acacggtgag attctttgtc gaaaatcagc ttggtcatac
    12001 cgtctgcgca gtcggaagcg atcgcacgac cagaagcagc ccacgggaag gtggcagttt
    12061 catagctgat gcctttctct ttcgcttctt tctcggtcag acccacccat gcaacttctg
    12121 gttcggtata ggcgatggac gggataactt tcggatcgaa gtagtgtttc ttaccggcga
    12181 taacttcagc ggcgacgtga ccttcgtgaa cacctttgtg tgccagcatc ggctgaccga
    12241 cgatatcgcc gatagcaaag atgtgcggta cgttggtacg cagctgtttg tcaacgcgga
    12301 tgaaaccacg gtcgtccact tccacgccag ctttgcctgc gtcgaggttt ttaccgttcg
    12361 gcacacgacc aatcgctacc agcacggcgt cgtaacgctg cggttcagcg ggtgcttttt
    12421 tgccttccat cgtcacataa atgccgtctt ctttcgcttc aacggcggta actttggttt
    12481 ccagcatcag gttgaatttc ttgctgatac gcttggtgaa gactttaacg atgtctttgt
    12541 cagctgccgg gataacctgg tcgaacattt caaccacatc aatctgtgaa cccagcgcgt
    12601 ggtatacggt ccccatttcc agaccgatga taccgccacc cattaccagc aggcgttctg
    12661 gtacttcttt cagttccagc gcgtcagtgg agtcccagat acgcggatct tcatgcggaa
    12721 taaacggcag ttggatcggg cgagaacccg ctgcaatgat cgcgttgtcg aagttgatca
    12781 cggttttgcc gttctcacct tcaacttcca gggtgttagc cccggtgaat ttacccagac
    12841 cgttgaccac tttgactttg cggccttttg ccataccagc cagaccaccg gtcagctgat
    12901 tgatcacttt ctctttccag gtacgaatct tgtcgatatc ggttttcggt tcgccgaaga
    12961 cgataccgtg ttcagccagc gctttggctt cttcgatgac ttttgctacg tgcagcagtg
    13021 ctttagaagg gatacagccg acgttcaggc aaacaccgcc aagggtgttg taacgttcta
    13081 cgattacggt ttccagacct aaatcagcgc aacggaaggc agcggagtaa cctgcggggc
    13141 ctgccccaag taccacgacc tgagttttga tttcagtact catcatgacc tctatatatt
    13201 tatctccggc ggtcataccc gtcgtctttc aggccgcagc ggcgttagct tcacgccctc
    13261 accccagtca cttacttatg taagctcctg gggattctgg cgcttgctac cttgctgcac
    13321 ccagaaatcc atagggtatg tgaagtaata ccctaaccac caccgggtcg ttctatccgt
    13381 cggtatttta caaaattgtt aacaattttt aaacaacaaa cggcaaccga tttgtctatt
    13441 cgctaataac ctcaatacat tcatgagatt accagaaaaa agccggccgt tgggccggct
    13501 cttttactta catcaccaga cggcgaatgt cagacagcgt gttgttaatg atggtaatga
    13561 aacgggcacc atcagcaccg tcgatcacgc ggtggtcgaa ggagagagaa atcggcagca
    13621 tcagacgcgg cacgaactct ttaccattcc acaccggctc catcgcggac ttggaaacgc
    13681 cgaggatagc cacttccggc gcgttcacaa tcggcgcgaa gtgggtagta cccaggccgc
    13741 cgatgctgga gatggtgaag caaccgccct gcatttcgcc cgcagtcagc ttaccgtcac
    13801 gcgctttctt agaaatagtc atcagctcgc gagacagctc gatgatgcct ttcttgttga
    13861 cgtctttgaa taccggaaca accagaccgt tcggggtatc caccgccaca ccgatgttga
    13921 tgtatttctt cagggtcaga cgctgaccgt cttccgacag cgaactgttg aagcgaggca
    13981 tctgctcaag agctgcagca acggctttca tgatgaagac aactggggtt atcttcacat
    14041 ccagcttacg tttcgccgct tcttcgttct gctgtttacg gaacgcttcc agctcggtga
    14101 tatcggtttt gtcgaagtgg gtaacatgcg ggatcatcac ccagttacgg ctcaggttcg
    14161 caccagaaat tttctggata cggcccagtt ccacttcttc gatttcacca aacttgctga
    14221 agtccacttt cggccacggc agcatgcctg ggataccgcc gccagtcacc gccggagccg
    14281 cttctgcacg tttgatagct tctttcacgt aagcctgaac gtcttcgcgc aggatacgac
    14341 ctttacggcc agtgcccttc actttcgcca ggttaacgcc aaactcgcgt gccagacggc
    14401 ggatcagcgg agtcgcgtga acgtaagcgt cgttttcagc aaattcagat ttgccttccg
    14461 ctttcacagc cggtgctgct gccggggctt cagcttttgc tgccggagcc ggcgctgccg
    14521 cttcctgttt cgcaggagct gccgcaggcg ctgcgccttc aacttcgaag atcataatca
    14581 gcgagccagt tttcacttta tcgccaacgt tgactttcag ttccttcacg acgcctgcaa
    14641 acggagccgg gacttccata gaagctttat cgccttctac ggtgatcagt gactgttcag
    14701 cggcaacttt gtcgcccact ttcaccatca cttcggtcac ttcaacttcg tcaccgccga
    14761 tatccggaac gttaacttct ttcacgccag ctgctgatgc aggggccgct gccggagctg
    14821 cttcctgttt agccgccgga cctgccgcgc ctgcttcacc cgcgacttcg aagaccataa
    14881 tcagcgagcc ggtagacact ttgtcaccca cgttcacttt gatctctttc acggtgcctg
    14941 caaacggagc aggaacttcc atagaagcct tatcgccttc tacggtgatc agcgactgtt
    15001 cagcttcaac tttatcgcca actttcacca ggatttcggt cacttcaact tcgtcgctgc
    15061 cgatatccgg aacgttaacg tcttttgccg ccgcagccgc tggtgctgct gccggagctg
    15121 cttctttctt ctcttctgcc tgagcaggtg cagcgtctgc tgcaccgtcg gcggaatcga
    15181 aaatcataat cagtgcgccg gtctgggttt tatcgccaac agagactttg atctctttaa
    15241 cgatacccgc ctgcggagat ggtacttcca tagaggcttt gtcgccttct acggtgatca
    15301 gcgactgttc ggcttcaact ttgtcgccca ctttgaccag gatctcggtg atttcaactt
    15361 catcagcccc gatgtccggt actttgattt cgatagccat tattctttta cctcttaagc
    15421 cagacgcggg ttaactttat ctgcatcgat gttgaatttg gcgattgcgt cagcaaccac
    15481 tttcttatcg atttcgccac gtttagccag ttcgcccagc gccgcaacca cgacataaga
    15541 agcatcaact tcgaagtggt gacgcaggtt ctcacggctg tcggaacgac cgaagccatc
    15601 agtacccagt acgcggtagt cgtcagccgg tacgtaagta cggacctgct cagcgaacag
    15661 tttcatatag tcggtagatg ccactgccgg agcgtcgttc atcacctgag cgatgtatgg
    15721 aacgcgcgga gtttccagcg ggtgcagcat gttccagcgt tcacaatcct gaccatcacg
    15781 cgccagctcg gtgaaggagg tcacgctata aacgtcagaa cctacgccgt aatctttcgc
    15841 caggatctct gccgcttcac ggacgtgacg caggatagaa ccggagccca gcagctgaac
    15901 tttacctttg ctaccttcaa tggtttcgag tttgtagata cctttacgga taccttcctc
    15961 agcaccttcc ggcattgccg gcatgtggta gttttcgttc agcgtagtga tgtagtagta
    16021 aacgttctct tgtttttcac cgtacatacg ctccagaccg tcatgcatga tgacagcaac
    16081 ttcgtaagcg taagccgggt cgtaagagat acagttcggg atagtcagcg actgaatgtg
    16141 gctgtgacca tcttcgtgct gcagaccttc gccgttcagg gtggtacgac cggaagtacc
    16201 gccgatcagg aagccacgcg cttgctggtc gccagccgcc cagcacagat cgccgatacg
    16261 ctggaagccg aacatcgagt aatagatgta gaacggaatc atcggcagat tgttggtgct
    16321 gtaagaggtc gccgctgcca gccaggaaca ccctgcgccc agctcgttga tcccttcctg
    16381 cagaatctga cctttctcgt cttctttata gtaagcaacc tgctcgcggt cctgcggggt
    16441 gtactgctga ccgttcgggc tgtaaatacc aatctgacgg aacagacctt ccataccgaa
    16501 agtacgcgct tcgtcggcga tgatcggtac cagacgatct ttgatcgact tgttcttcag
    16561 catcacgttc agggcacgaa cgaaagcgat agtggtagag atctctttgc tctgctcttc
    16621 cagcagcgcg ccgaagtctt gcaggctcgg cagctcaagc ttctcggtga agttcggctg
    16681 acggcttggc agataaccgt gcagtttctg acgctgagcg tgcagatagg tatgctcttc
    16741 agaaccttcc gggaaggtga tgtacggcag tttttcgata tctgcatcag acaccggcac
    16801 attgaaacgg tcgcggatat gacgcacgcc gtccatgttc attttcttaa cctggtgcgc
    16861 gatgttttta ccttcagccg cgtcgcccat gccgtaacct ttaatggtat gagcaaggat
    16921 taccgtcgct ttgcctttgg tttcctgcgc tttcttgaat gcagcgtaga ttttcttcgg
    16981 atcgtgaccg ccacggttca gcgcccagat ctgctcgtca gtccagtctg caaccagagc
    17041 tgcggtttcc ggatatttac cgaagaagtg ttcacgaacg tacgcaccat ctttcgattt
    17101 gaaggtctgg tagtcgccgt caacggtttc gttcatcagc tggatcagtt taccgctggt
    17161 atctttacgc agcagttcat cccaacggct accccacatc actttgatca cgttccagcc
    17221 agcaccttcg aagatgcctt ccagttcgtt gatgatcttg ccgttaccgg tgaccgggcc
    17281 gtcaagacgc tgcaggttac agttgataac gaagaccagg ttatccagtt tttcacgggt
    17341 agcgatggtg atcgcacctt tggattccgg ttcgtccatt tcaccgtcgc cgaggaaggc
    17401 gtaaacggtc tgtttagagg tatctttcag gccacggtgt tccagatatt tcaggaattt
    17461 agcctggtaa atagcaccaa tcggacccag acccatagat acggtcggga actgccagaa
    17521 ttccggcatc agtttcgggt gcggatagga agagaggcca ttgccgtgaa cttcctgacg
    17581 gaagttatcc agctgctcct gagtcagacg accttccagg aaagcacgag cgtaaacgcc
    17641 cggggagatg tggccctgga agtaaaccag atcgccgcca tcctgctcgt tgcgtgcacg
    17701 gaagaagtga ttaaagcaca catcataaat ggttgcggaa gactggaagg acgccatgtg
    17761 gccgcccagt tcgaggtctt ttttcgacgc acgcagaacc gtcatgatgg cgttccagcg
    17821 gatagctgaa cgaatacggc gttccagttc cagattaccc ggatactccg gttgttcttc
    17881 aacggggatg gtgttgatgt agttgctgat accggtgcct gcggctacgt tgacaccgcc
    17941 tttgcgggct tcagcaagca gttggtcgat cagatactga gcacgctcaa caccttcttc
    18001 acggatgacc gattcgatcg cctggagcca gtcgcgagtt tcgatcggat ccacgtcatt
    18061 tgggaaacgt tctgacatgg gttattcctt atctatctaa taacgttgag ttttctggaa
    18121 cctgtcccat tgaactctcg ccggaaagct caataagaca ggttctacgt ttagttgccg
    18181 cgcttttata tgcgcttgat ttacaacatc ttctggataa tttttaccag aaaaatcact
    18241 aattctttcg ttgctccaga cgacgcagag aacgctcacg gcggctctct tcacggcttc
    18301 tgtcgagcaa aatttcttcg ataaaggcca gatggcgatg cgatgcttcg cgcgcttctt
    18361 ccggcttacc ggccataatc gcttcaaata tgcgggtgcg gtgactactc accagcggca
    18421 gcatctcgcg acgcgaatag agcaattcga agttctggcg gacattctgg gccaacatcg
    18481 gctccataca ccttagcaga tgaagcagaa ccacattgtg ggccgcttca gtgacagcaa
    18541 tctgatactg gagtacggcg tttgattccg cgtccagatc gccagactgc tgcgccagct
    18601 ctatggcgtg atggagttca cggatgcgtt ccttgtcttc atcggtacta cgcagcgcgg
    18661 cgtaataagc ggcgatacct tccagggcgt gtcgtgtttc gagcaagtca tactgtgatt
    18721 caggatgatc ggagagcagc tctaccagcg gatcgctgaa gctttgccat aggctgctct
    18781 ggacaaaagt gccgccaccc tggcgacgaa gcaacaagcc cttcgcttcg agacgttgaa
    18841 tcgcctcacg caaggaggga cgggagacgt caaactgttt tgccagttcg cgttccggtg
    18901 ggagtttttc gcccgggcgg agagtgcctt cgaggatcaa aaactccagt tgctgctcaa
    18961 tcacatcgga gagttttggt tggcggattt tgctgtaggc catgagttcc tgtcttaagc
    19021 cacttgccga agtcaattgg tcttaccaat ttcatgtctg tgacgctaaa gtaacaaagt
    19081 attcacctta tgtccataca ggttttgatt gaaatcatga aactgtgcac attttaacaa
    19141 cttgacatat ataacgtttc aaagttgtaa ctatgcacaa atgtagactt tacgtaggaa
    19201 aggg
//
LOCUS       DACBVI010000085        19174 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.085, whole genome shotgun sequence.
ACCESSION   DACBVI010000085 DACBVI010000000
VERSION     DACBVI010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.085"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..894
                     /locus_tag="IU325_14345"
     CDS             <1..894
                     /locus_tag="IU325_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP5810185.1"
                     /translation="LYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQSLQR
                     LRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGIEQTINIVNKSELKKNFIIYGP
                     QLISCTNNNMLIRCLRQDTSIDIECHDILLSAENAEELLVQRKTDLAITLQPVISRSV
                     ICMPLHTIRNSLICSNKHPRITDTSNYEQIIAEEFTLLISKSAGIDEIQMDIDERFMN
                     RKVSFRSSSLLTIINSISVTDLLGIVPTELYDLHRDFLKLQEIKLEQPLPAVKLYISY
                     NKASLNNLVFSRFIDRLNDSF"
     gene            1041..2261
                     /locus_tag="IU325_14350"
     CDS             1041..2261
                     /locus_tag="IU325_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HAP5810186.1"
                     /translation="MSIYKIPLPLNILEAAKERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YASHQRLCNPLYNIMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCDND"
     gene            2234..2863
                     /locus_tag="IU325_14355"
     CDS             2234..2863
                     /locus_tag="IU325_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HAP5810187.1"
                     /translation="MGDTMRQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(2864..4024)
                     /gene="ybdL"
                     /locus_tag="IU325_14360"
     CDS             complement(2864..4024)
                     /gene="ybdL"
                     /locus_tag="IU325_14360"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HAP5810188.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRIALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALSESRLEILPCEGTYFLLVDYSAVSSLDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            4133..5221
                     /gene="hcxA"
                     /locus_tag="IU325_14365"
     CDS             4133..5221
                     /gene="hcxA"
                     /locus_tag="IU325_14365"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HAP5810189.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSLNHLHDFFTDEQLSRAVWIYGER
                     AIAAAQTKLPPAFERPGAKHILFRGHCSESGVQQLAADSGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHFEIFDDANFMVLVEPEIIL
                     NAPQEYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAICDVLLNSSEQALS
                     DQQKQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDNVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(5231..5428)
                     /locus_tag="IU325_14370"
     CDS             complement(5231..5428)
                     /locus_tag="IU325_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenoprotein"
                     /protein_id="HAP5810190.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(5556..7661)
                     /gene="cstA"
                     /locus_tag="IU325_14375"
     CDS             complement(5556..7661)
                     /gene="cstA"
                     /locus_tag="IU325_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HAP5810191.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7842..8255)
                     /gene="entH"
                     /locus_tag="IU325_14380"
     CDS             complement(7842..8255)
                     /gene="entH"
                     /locus_tag="IU325_14380"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HAP5810192.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTLDGQCVVGTELNATHHRPVSEGKVRGIC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(8258..9004)
                     /gene="dhbA"
                     /locus_tag="IU325_14385"
     CDS             complement(8258..9004)
                     /gene="dhbA"
                     /locus_tag="IU325_14385"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase"
                     /protein_id="HAP5810193.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(9004..9861)
                     /gene="entB"
                     /locus_tag="IU325_14390"
     CDS             complement(9004..9861)
                     /gene="entB"
                     /locus_tag="IU325_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HAP5810194.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9875..11485)
                     /gene="entE"
                     /locus_tag="IU325_14395"
     CDS             complement(9875..11485)
                     /gene="entE"
                     /locus_tag="IU325_14395"
                     /EC_number="2.7.7.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase"
                     /protein_id="HAP5810195.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNRAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(11495..12670)
                     /gene="entC"
                     /locus_tag="IU325_14400"
     CDS             complement(11495..12670)
                     /gene="entC"
                     /locus_tag="IU325_14400"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HAP5810196.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAKGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPDQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLHKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSHENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12859..13815
                     /gene="fepB"
                     /locus_tag="IU325_14405"
     CDS             12859..13815
                     /gene="fepB"
                     /locus_tag="IU325_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP5810197.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIVAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQALLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIVQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13819..15069)
                     /gene="entS"
                     /locus_tag="IU325_14410"
     CDS             complement(13819..15069)
                     /gene="entS"
                     /locus_tag="IU325_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HAP5810198.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVICLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            15180..16184
                     /gene="fepD"
                     /locus_tag="IU325_14415"
     CDS             15180..16184
                     /gene="fepD"
                     /locus_tag="IU325_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HAP5810199.1"
                     /translation="MSGSVAVTRAIAVPGLLFLLIIATALSLLIGAKSLPASVVLEAI
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            16181..17173
                     /gene="fepG"
                     /locus_tag="IU325_14420"
     CDS             16181..17173
                     /gene="fepG"
                     /locus_tag="IU325_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HAP5810200.1"
                     /translation="MIYVSRRLIITCLLLITACVVAGIWGLRSGTVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            17170..17985
                     /gene="fepC"
                     /locus_tag="IU325_14425"
     CDS             17170..17985
                     /gene="fepC"
                     /locus_tag="IU325_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HAP5810201.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTAKI"
     gene            complement(17982..19115)
                     /gene="fepE"
                     /locus_tag="IU325_14430"
     CDS             complement(17982..19115)
                     /gene="fepE"
                     /locus_tag="IU325_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HAP5810202.1"
                     /translation="MSSLNIKQGSDAHFPEYPLASPSNNEIDLLNLIAVLWRAKKTVM
                     AVIFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSTPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTNEEAQTVLSGYIDYISALVVKESIENERNRLELKTQFE
                     KEKLAQDRIKMKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSAMIGGMVACGGVLLRYAMASRKQDAVMEEHLV"
BASE COUNT         4580 a         5375 c         4866 g         4353 t
ORIGIN      
        1 ctttatgacc taaaaaaatt tgatctgaat ttactggtca tatttgagtg tatttatcaa
       61 catctcagca ttagtaaagc ggcagaatca ttatatataa cgccctctgc tgttagtcag
      121 tcgttacaac gtttacgtgc gcaatttaat gacccgttat tcattcgctc agggaaaggt
      181 atcgcgccga ctacgacagg gttaaatctg catcatcatc ttgagaaaaa tctcagaggc
      241 attgaacaaa cgatcaatat tgttaataag tctgagctaa agaaaaattt tattatctac
      301 ggtcctcagc ttatttcttg tactaataac aacatgctaa tccgctgtct tagacaagat
      361 acttcaatcg atatagaatg ccatgacata ctgctttcag ctgaaaatgc cgaagaactg
      421 ttggttcaac gcaaaactga cctggcaatt acactacaac cagtaattag ccgctctgtc
      481 atctgcatgc cgttacatac tattcgcaat tctcttatat gtagcaacaa acatcctcgt
      541 attaccgata ccagtaacta tgaacaaatt atcgccgaag aattcacctt gttgatatct
      601 aaatcagcag ggattgacga aattcaaatg gacatagatg aacgttttat gaaccgtaaa
      661 gtttcatttc gcagttcttc tttgttgacg ataattaata gtatttctgt tactgactta
      721 ttgggcattg tgcccaccga actgtacgat ttacatcgtg attttttgaa gttacaagag
      781 ataaaactgg aacaaccact tcctgcggtt aaactttata tttcctataa caaggcttcg
      841 ttgaacaacc ttgtcttctc acgattcata gatcgtttaa atgatagctt ctgatttatt
      901 accactgata aatgttatat tacgcatcaa ttgattcatt gtgtaaatat aaaataatgt
      961 gctctatctt gcttcgttta ccctataatt agcggattca tctcacgagt caccccgtaa
     1021 cgttcagaga gcgttttcag atgtctattt ataaaatccc gcttccactg aatattctgg
     1081 aagctgcaaa agaacgtatt acctggacac ttaacaccct accccgcgtc tgcgtttctt
     1141 tttcgggtgg taaagattcc ggattaatgc tgcatctgac cgccgaactt gcccgacaaa
     1201 tgggcaaaaa aatttgtgtt ttatttatcg actgggaagc tcagttctct tgcaccatta
     1261 actatgttca gtccctgcgc gagttgtaca ccgatgtcat cgaagagttt tactgggttg
     1321 cgctcccgct tacaacgcaa aattccctat cacaatacca acccgaatgg cagtgctggg
     1381 aacctgatgt cgaatgggta cgtcagcctc cgcaagatgc gataaccgat cctgactttt
     1441 tctcctttta ccagccaggc atgaccttcg aacaatttgt gcgtgagttt gccgaatggt
     1501 tttcacaaaa acgtccggcg gcgatgatga tcggcatccg tgcggatgag tcctacaacc
     1561 gttttgtcgc catcgccagt ttaaataaac aacgttttgc cgatgataag ccctggacca
     1621 ctgccgcacc aggcggtcat agctggtaca tttaccccat ctacgactgg aaagtcgctg
     1681 atatctggac ctggtatgct agccatcaac gcctctgtaa cccactgtat aatataatgt
     1741 atcaggcagg cgttcctcta cgtcatatgc gaatttgcga accttttggt ccggaacaac
     1801 ggcaaggatt atggctctat cacgttatcg aaccggatcg ctgggctgct atgtgcgcac
     1861 gagtcagcgg ggtaaaaagt ggcggcattt acgccggaca tgacaaccat ttttatggtc
     1921 atcggaaaat cctcaagccc gaacatttag actggcagga gtatgcatta ttgctactca
     1981 acagcatgcc ggagaaaaca gctgagcatt atcgcaataa aatcgccatt tatttgcact
     2041 ggtatcagaa aaaaggcatc gaggttccac aaacccagca aggggacatt ggcgcgaaag
     2101 atatcccctc ctggcggcga atatgcaaag ttttactcaa taacgattac tggtgtcggg
     2161 cattatcatt tagcccgacc aaagcgaaga actatcagcg ttataacgaa cgaataaaag
     2221 gaaaacgtca ggaatggggg atactatgcg acaacgatta acgcaggatt taacccaatt
     2281 tctcgccagc ttgccggaag acgatcgcat caaagcgatt aatgaaatcc gcatggcgat
     2341 ccatcaggtt agcccctttc gagaagagcc tgtggattgc gttctgtggg ttaaaaacag
     2401 ccaacttatg cctaatgatt acaacccaaa caacgtggca ccacccgaaa aaaaactgct
     2461 gaaaaaatcc attgaaatag atggttttac ccaacccatt gtggtcacgc atacggataa
     2521 aaatgccctg gaaatcgtgg atggttttca ccgccatgaa attggtaaag gctcatcatc
     2581 gttaaaatta cgcttaaaag gttatttgcc ggtgacctgt ctggaaggta cgcgtaatca
     2641 acgcatcgcc gcgactattc gtcataatcg cgcccgtgga cgtcaccaaa tcactgccat
     2701 gtcagaaatc gtccgtgaac tcagccagtt gggatgggac gataataaaa ttggcaaaga
     2761 gctgggaatg gacagcgacg aggtgctgcg cctgaaacaa attaatggcc tgcaagagtt
     2821 gtttgccgac cgtcaatatt cccgtgcctg gactgtcaaa taactacagc tggcgcaggc
     2881 gttcagctgc tgccagcaac gtcgattcct tcttggcaaa acagagacga atcagtttat
     2941 gggggaaggg atcggcgcaa aataccgaca gcgggatcgc cgctacaccg tgctcccgcg
     3001 tcagccactg gcaaaactca acgtcatcca gcgaagaaac cgcgctgtaa tccaccagca
     3061 aaaagtaagt gccttcgcac ggtaaaatct ccagccgact ctcacttaag gcattcacca
     3121 gaatatcgcg tttttggcga taaaagtccg gtaacgcaag ataatgctca ggttctgcac
     3181 gtagcatatc cgcaagtgcc agctgtgccg gggtattcac cgaaaaggtc agatactggt
     3241 gtaccttgcg aatttcagcg ctgatgggcg ctggcgcaac gcaataaccc actttccagc
     3301 cggtcatatg ataggtcttg ccaaatgaag aaactgccac cgcccgctca cgcagctgcg
     3361 gatgcgccag cacgctggca tggccctgtt gtgaaaagtt gatgtgctcg taaacttcat
     3421 cgctaatgac aaaaatctcg tgcccggcaa ttgcctgcca caaagcggcg aaatcagcct
     3481 gctgccagac agttgcactg gggttatgcg gggtgttgag gatcaccagt cgggtgcgct
     3541 cgcttaacaa tgcggcaaat tcctgccagt ccacgcgaaa atgcggtggt tgcagtgcaa
     3601 tacgtttcac tattcccccc gaaagcgcga tggcgggcgc gtaactgtca tagctgggat
     3661 caaagcagat cacttcatca ccattgcgca ccaatgcggt aatcgctgca tataacgctt
     3721 ccgtcgcccc tgccgttacg gtgatatcgc tatcggcatc cggttgatag ccatacagac
     3781 gttccgtttt ctgagcaatc gcctcgcgca aggcctgcac gccagtcata ggcgcgtatt
     3841 ggtttgcccc ctgggcaacg tggtacgcca ggcgctcctg caaatagcgc ggaccatcaa
     3901 aatcaggaaa gccttgcgac aggttaattg cctggtgttg ctgcgccagc gcgctcatct
     3961 gggtgaaaat agtggtgcca agttgtggaa gtttgctttg tggaatcaga gggttatttg
     4021 tcatttttat tgtacctgac gaagttgcgt gttgaaggca ctataacacg atgctagtat
     4081 ttggcaatca agacgtttag atgtctaaat aaaacaataa ggacaacaca acatgcctca
     4141 caatcctatc cgcgtggtcg tcggcccagc taactacttt tcacatccag gcagtctcaa
     4201 tcacctgcat gattttttca ctgatgaaca gctttctcgc gcggtgtgga tctacggcga
     4261 acgcgccatt gcagcggcgc aaacaaaact tccgccagcg tttgaacggc caggggcaaa
     4321 gcatattttg tttcgcggtc attgcagcga aagcggtgta caacaactgg cggctgactc
     4381 cggtgacgat cgcagcgtgg tgattggcgt cggcggcggc gcactgctcg acaccgcaaa
     4441 agccctcgcc cgccgtctcg gtctgccgtt tgttgccgtt ccgacgatag ccgctacctg
     4501 cgccgcctgg acaccgctct ccgtctggta taacgatgcc ggacaggcgc tgcattttga
     4561 gattttcgac gacgccaatt ttatggtgct ggtggaacca gagattatcc tcaatgcgcc
     4621 gcaagaatat ctgctggcgg ggatcggtga cacgctggcg aaatggtatg aagcggtggt
     4681 gctggctccg caaccagaaa cgttgccgct aaccgtgcgg ctggggatca ataatgcgca
     4741 ggccatttgc gacgtcttgt taaacagtag cgaacaggcg ctgagcgatc agcaaaaaca
     4801 acagttaacg caatcatttt gcgatgtggt ggatgctatt attgctggtg gtgggatggt
     4861 cggtggtctg ggcgatcgtt ttacgcgtgt ggcggcggcc catgccgtgc ataacggtct
     4921 gaccgtgctg ccgcaaaccg agaagtttct tcacggcacc aaagtcgcct acggaattct
     4981 ggtgcaaagc gccttgctgg gtcaggatga tgtgctggca caattaaccg gggcgtatca
     5041 gcgttttcat ctgccgacta cgctggcgga gctggaagtg gatatcaata atcaggcgga
     5101 gatcgacaac gtgattgccc acaccttacg tccggtggaa tccattcatt acctgccagt
     5161 cacgctgaca ccagatacgt tgcgtgcagc gttcgaaaaa gtggaatcgt ttaaagcctg
     5221 acgtacacat ttagcagcag cgcgcgccgc cttttccacc gtagcgcgcg tcctgccgct
     5281 cacggaaaaa ctcttcgtag gtcatcggcg tttgatcggg atggttaacc cgcatatgtt
     5341 cgacatagtt gtcgtaatca ggcataccaa tcatcagctt cgccgcctga cctaaatatt
     5401 ttccggcttt tgccagtgaa tcaaacatca atacctccgg taaacaggtt gctgaaatat
     5461 tgccggatac ggcgcgaaca ccttatccgg cctggaattg ctgagagtaa ggggaaccct
     5521 cggcactatg ccaacattcc cccctcactc tgactttagt gcgcgccttt tgcctgcgcc
     5581 acgatctcct cgacattttc cggcattggc tcatacggcg tttctttcgc cgttggcttc
     5641 ggctctttca atgccgccag tgccgtctta atcgagaaca gtgccagaac caccacgacc
     5701 accataaaga agatggttaa cccggcatca agacggttgt tgaacaccag ttgcgccagt
     5761 tgtgactcag tatactgcga cggaatattg ccgctgtcga tcattgcctg gaacttatta
     5821 gcaatcgcca ggaagccgac tttcgcatcc gggctaaacg ctttctgcca gcctgcggtc
     5881 agggtacaaa tcagcagcca ggccgttggt accagcgcca cccaggcgta acgttgacgt
     5941 ttcattttga acagcaccac agcacagagc atcagcgcca tccctgccag catctggttg
     6001 gcaataccaa acagcggcca caaggtgtta ataccgccca gcggatcgac cacgccctga
     6061 tgcaggaagt agccccacgc cagcacgcac agcgccgttg ccagcaggtt agcaggcagt
     6121 gaatcggtcc gtttcaggcc cggagacacc acgcccagca gatcctgcaa cataaagcgc
     6181 gcagcacgcg tacctgcatc caccgccgtc agaataaaca gtgcttcaaa cagaatggcg
     6241 aagtgatacc agaacgccac atccatcatg ccgcccagcg caccgtgcag aatgtaggcc
     6301 atccccaccg ccagggtcgg cgcaccgccc gcacgggaaa tgatcgactg ttcacccact
     6361 tcgctggcaa tctggtttag cgtatccggg gtaatcgcaa agccccagct actcaccacc
     6421 tgcgcggcag aagcaaccac atccgccgtc cctgccggag ccagcaccgc catcgggctg
     6481 ttcatggcga agtacacacc cggatcgatg atacaggcgg aaaccagcgc cataatcgct
     6541 acgaaggatt ccattaacat cccgccgtag ccgataaaac acgcctgccc ttcgttcgcc
     6601 agcatcttcg gcgtggtgcc ggaagagatc agcgcatgga agccagacac cgcgccacag
     6661 gcgatggtga taaacaggaa cgggaacagg ttaccggtcc ataccgggcc agtaccatca
     6721 acaaatttgg tcagcgcagg catggtcagc gtcgggcgca taatcaaaat gcctaccgcc
     6781 agaccgacga tagtcccgat tttcaggaag gttgagaggt agtcacgcgg agccagcagc
     6841 agccacaccg gcagcaccgc cgccacaaaa ccgtaaccca ccagcatcca ggtcagctgc
     6901 acgccggtaa agtcaaagta cggtgcccag gtcgggcttt ctgccaccca gccgccagag
     6961 ataatggcga aaatcaggaa caccagacca atcaccgaca cttcgccaat acgccccgga
     7021 cgcaaatagc gcaggtagat ccccataaac agcgccagcg gaatggtgaa cgcaacggta
     7081 tacgttcccc acgggctatg agtcagggct ttcaccacga tcatcgccag tactgcaagg
     7141 ataatgacca tgatcataaa gcaggccacc agcgcaatca ccccggcggt tggccccatc
     7201 tcttctttga ccagctcacc cagcgagcga ccgtcacggc gcgtagaaac aaacagcacc
     7261 atgaaatcct gcaccgcacc ggcgagaacc accccggcga gcagccagat catccccggc
     7321 aggtagccca tttgcgccgc cagcaccggc ccaaccagcg gacctgctcc ggcaatggcc
     7381 gcaaaatggt gaccgaacag cactttcttg tccgtcggca catagtccag cccgtcgtta
     7441 tggcgcacag ctggcgtcat acgcgtcggg tcaaccgcca gcacattttt ggcgatatac
     7501 aggccataaa aacggtaagc gatcagatag atacagaccg acgccaccac aatccacagc
     7561 gcgttgatct gttccccacg atttaaagca atgtatccca gagcaaatgc tcccattaca
     7621 gagagcactg tccagacgag gtatttccct gatttgttca tagttgttat ccgtgtgcgt
     7681 gtccatagag atgttacatt ttgtttctat aacatctttg tacaatttaa caacacagca
     7741 accatgtaaa tcggagttga aaccaatatt taacttaaca atgttaactc gatcactccg
     7801 ttaaccgagt tttcctgcaa tctcatttaa ttctgtcggg ctcatcccaa aactgccgta
     7861 cccagccgac aggtgcagca acgccgcccc tgttcatcga aaacaacgat ttcccagctc
     7921 tgattttgcc gcccaagatg cagcggctgg cagatgccgc gtaccttgcc ttcagacacc
     7981 gggcgatgat gtgtggcgtt aagttccgtg cccaccacac actgtccgtc gagggtcatc
     8041 ataaatccgg ccatcgatcc cagcgtttcc gccagcgccg ccgacgcgcc gccatgtagt
     8101 aaaccgaacg gctgatgagt acgggtatcg accggcattt cggcttcgag tacatcatcg
     8161 cccagacggg tatacacaat tcccagatgc gccaccattg tgttatcgct ggtggcgttc
     8221 agctcgtcga gcgttaaatg gcgtttccag atcatgctta tgcccccagc gtcgagccgc
     8281 catctaccac aatatcctgt agcgtgatat ggctggcgag gtcagaggcg aggaacaaaa
     8341 tcgtgttggc gatctcttgt ggacgggcga ttttccccag cggaatgccg agtttaaact
     8401 gctcgccaaa gccgcgaata cgctgctctt cggcgtcatc gctcacccac agcgtgcgtt
     8461 gcatatcggt gtcggtggag cctggcgaaa ccacattaca gcgcacgccg ctacccgcca
     8521 gttccagccc aacgctcaac gccaggcttt tcagcgccgc tttcgacgcg ccataagcac
     8581 tcataccaat gcgcggcgtg tgtgcggcgt cggacgcaac agtaacaatc gccccgcccc
     8641 gctgacggcg aaactggttc atggtctgct ggaacaggtt aaacgcaccg ccgacattga
     8701 ccgcaaaagt ctgctgccag tcctccttac tgagctgatc ggtcgcgccc atgcgcaaaa
     8761 ttcccgccgc attgaccagc acgtccagtc gctccgtttc agctaacagt cgctgacata
     8821 cctgcgccac ctgcgcagca tcggcaacat ccattacttc ggtcgcaaaa ggatattgct
     8881 cctgagtaaa cgcttgatca aaacctgtaa ctttcgcccc cgcctcaaca aatgccagcg
     8941 ccgtagcata gccgatacct ttccccgcgc cagttaccca gacattttta ccgctgaaat
     9001 ccattatttc acctcgcggg agagtagctt ccaccaggcg tcgatggtcg ggtttttcgc
     9061 cagcatgaca aagtcgatat cgccatgcac tttgcgccag cgcgccgcca gcgccatcat
     9121 gcgcaccgaa tccagaccgt agtcgatcag gttgtcgtca tcgaacggtt catcggactc
     9181 gtccagcaac ggcaggatca cctcacgcag cgccgctttg ctggcgggga taggtgctgg
     9241 cagtaattct tcggtcatca ccacccggcc agaacgtccg gccacatatt tcagcgacat
     9301 caaatgctcg tcacggctga aatcagccag cgcatccgcc accataaacg gtttaatatc
     9361 gcgcataaat gcgtcggttg cggtggtcat acagccaatg tgggcatata ccccggtaat
     9421 aatcagctgg ttacgtccac tctctttgag catttgctcc agcggggaac gatgaaacgc
     9481 gctgtagcgc cacttcacca gcaccgtgtc gtcggcatct ggcgtcaggc gatccaccac
     9541 cttttgctgt tccggcgaac gggtcaggcc aggcccccac atatcattca acagcgcccg
     9601 atcttcatcg ctctgctctt tcggctgggc ggtgtaataa accgggatat tgtgctgttt
     9661 gcagtagtcg cgtagtgccg caatattcgc gatcacctgc tccatcatcg ggcagttctc
     9721 gccccagaag ctgacaaaat agtcctgcat atcatggatt aacaacgcgg cacgttgcgg
     9781 ttcaaaggcc cagtcaactt tattctgcgg aatatcgtga gactccggca gtgcgtaagc
     9841 ctgtaatttt ggaatagcca tcgtgttctc tccttcaggc tgatgcgcgt gacgccagcc
     9901 actgacgtaa ttgtttttta tcgactttcc cgaccgccgt cagcggaagt gaatccacac
     9961 actccacgcg atccggtaat ttaaattcgg caataccctg ttcacgcagg aaacgacgca
    10021 cctgcaccgc gcgtagaggc tcttttacca ccagataagc acagcttttt tcgcccatca
    10081 gctcatcttc catgctcacc agtgcggcat agatcaccgc cggatggcgc agcagcaggt
    10141 tttcgatctc ttcggcagcg atcttctcgc cgccacggtt gatctgatct ttctcgcgcc
    10201 cctgcacggt gatgtaaccc tctggatcaa tagagatcag atcgccggaa cagtaaaaac
    10261 cgttggcatc aaaggcactg gcattgtgct gcgggctttt gtaataaccg cggaaggtgt
    10321 acggcccgcg cgtcatcagg cgtccaactt ccccttgcgg cagtggattt ccttcggcat
    10381 cggcaaccca cacttcatcg tccgggcaca ttgggtaacc ctgggtatgg ataattttct
    10441 ccgcgctatc atcaagacgg gtgtagttca ccagcccttc cgccatgcca aacacctgct
    10501 gcaactggca gccaatctca gcgggaatac gcgccgcaag cgtggcagaa agacgtgcgc
    10561 cgccgacctg taacagtttc agcgaggcaa gctgcgcccg gctttcgcct tcggtcagcg
    10621 cctgtaacca caggctgact gcgggtggca ccagcgcggt gacgttcacc tgatgttttt
    10681 caatcaatgg gaaacagagc gtggcgctgg gatcggccgc cagaacgacc gttcctccgg
    10741 caagaaagac gcccagcgat cccggcgaac tcatggcgta gttatgagcc gccgggatcg
    10801 cgcacaggta gcgtgtctgt tgtgtgaact gacaaatctc gacgctacga cgcacgctgt
    10861 agtagtagtc gttatgagtg cgcgggatca gtttcggtgt gccggtggtg ccgccggaaa
    10921 gctggaaata ggccacttca tcagcaggtg acggcgtggc agtaaaatcc tcagccggat
    10981 ggttaatcgc atcctgcaaa ttatgctcac cgctgtcgtt gtgcagttgc accacgcgaa
    11041 tggaggaatg ctctgcaaca aatgtattga ggaaatcatc cccgctaaac agcgcatgct
    11101 ggcgatcggc aatcagtaac gccggttcaa tctggctggc ataggcgttc agttcactac
    11161 gctgatggct gaacagcgcc agcaccggcg caacgcccag cttcagcagc gcgaaaaagg
    11221 taatatacaa ttcagcgacg ttacccagct gtaccagcgc ggtttcacca ggtttaatgc
    11281 cctggcggcg caaactacag gcgaggttat ctgccgcccg attcagctcc cggtaactca
    11341 actgccgctc gccgtcgatg accgcgatgc tgtcgcttgc ggcatgacgg gtcagaatgt
    11401 cggtcagcgg caaatcctgc cagtagcctt tttcccgata gcgacgggca aactcttccg
    11461 gccagcgggt gaatggaatg ctcatcctca ctccttaatg caatccaaaa acgttcaaca
    11521 tggtagaaag tttgacgccc gtttcgcgcc actcacccaa cggtgacgac gcaggcacaa
    11581 tccccgctcc ggcaaacaga cgcacctgat tttcccgcag cttcgcgcag cggatggtca
    11641 ccacccactc gccgttacct tcgctgtcac accaacccac aatgccgcca aacagttcgc
    11701 ggtcgaacgg ctccagttca gcaataacct gggtcgcggc ctgatgcggg aaaccgctca
    11761 gcgcgggggt cggatgcagc agacaggcca gcgtcagtgc gttctcatgc gaattcgctt
    11821 taccttcaaa gggagttgcg agatgccaca gcgtcggcgt ggtgatcagt tgtggagaag
    11881 aagggacgtg taactcacta cttcgttcgc gcagtacctc tttcatcgcc tgagtcacca
    11941 gttcatgttc atggcgatct ttttctgacg ccagcagacg attacccgct tcgcgatcga
    12001 gcacttcatc cggctgacga cgcgcggaac cggctaacgg aatggagcta aaacgctcgc
    12061 cgtctttatg tagcagcagt tccgggctgg cccccagcag aacgccacca tcagccagcg
    12121 ggacatggaa gttgtaacta accgggtttt gcgcaatcaa ccgttccagc aatacgccac
    12181 tatcaatggc ggcgtcagtg gtgatatcaa tcaaccgtga caacaccact ttgtcgacct
    12241 gcggcgtggc ggtaagtgcg gcggcgcgag caaccatctg ttcaaacgtg gtttgatccg
    12301 gaattgcctg acgttccacc acgttcagcg actggctgcg ggtgaaacgg cgtgcggaag
    12361 cttgtttttc ctggcgggag aacgactgcc aggattcagg aatatacagc gatgaaggct
    12421 gacgtggatc gaagggaatc gccccgacca tcaccggatt tttgatgccc ttcgctttgg
    12481 catcggcaaa cagcgcggcg agtttttgct ggaagggact gtcgggcgaa tccccgttca
    12541 cagccggttc atcgaagcgg gcgaaacatc ctgacgtcgt aaaactgcgg tacggcgaca
    12601 taaagaaaaa gcgattgggc gcaagtgttg ccatggtctg ctgtacttcc tcagccagtg
    12661 acgtatccat atcatcctcc acaaaatgat aaaggcttta ataatgatta tcatttatat
    12721 tttcggtcgc taacctaaaa gcaaacgcgc actatgtcaa ctcttgaggt aacgcaatct
    12781 gcaactcggg gttgcatctg gttgtggtta caatgaaaat gagaagcatt aacttcatta
    12841 ataacaggaa gctgacttgt gagactcgcc ccgctctacc gcaacgccct tctattaaca
    12901 ggacttttgc tttcaggaat agtcgcagtt caggccgccg actggccgcg tcagattact
    12961 gacagccggg gcactcatac cctggaaagc cagccgcagc gtattgtttc caccagcgtc
    13021 accctgaccg gctcactgct ggcgattgat gctccggtga ttgccagcgg cgcgaccacg
    13081 ccgaataacc gcgtcgcgga tgaccagggc tttttacgcc agtggagcaa ggtggcgaaa
    13141 gaacgcaaac tgcaacggct ctatatcggc gaaccgagcg ccgaagccgt tgccgcgcaa
    13201 atgccggatc tgattttaat tagcgcaact ggcggggatt cggcgctggc gctgtacgat
    13261 cagctttcta ccatcgcccc aacattaatc atcaattacg acgacaaaag ctggcaggcg
    13321 ctgttaacgc aacttggcga aattaccggg catgagaaac aagcggcaga gcggattgca
    13381 cagtttgata agcaactggc ggcggcgaaa gagcaaatca aattaccgcc gcagccggtc
    13441 actgccattg tctataccgc cgctgcacac agtgccaatc tctggacgcc ggaatcagca
    13501 caagggcaga tgctggaaca actcggcttt acgttggcga aattgcccgc agggttaaac
    13561 gccagccaaa gccagggcaa acgtcatgat atcgttcagc ttggtgggga aaatctggct
    13621 gcagggttaa atggcgagtc actattcctg ttcgccggtg atcagaaaga tgccgatgct
    13681 atttatgcta atccgctgct cgcgcacctg cctgcagtac aaaacaagca ggtttatgcg
    13741 ctgggaaccg agacgttccg actggattac tacagcgcca tgcaagtgct ggaacgactt
    13801 aaggcgctgt tttaagcatt aactgtcgga cgctgtcacc tgcggcggcg tctggcgaaa
    13861 acgtcgcaac tccaccagca ccagcaataa caacacaccg ataatcaaca aaccaaaacc
    13921 gctcgcgctt gcggaagcaa ccggtgtcat catcgctccc agaccgccta acagcgccgc
    13981 acctatggca tcgcccgtca cgttttgcgc cgtccacaaa ccgttaatcc gccctaacat
    14041 cgcttccggg gtttgcgttt gcagcattgt gtattgcagc aacgagctga ccgcgctcag
    14101 ccagccgaat agcgccagac aaatcacgcc taaaatccac atcggcatca ggccaaacag
    14161 cccaatggcg aggaacgatc ccagcgtgga gagcagcatc aataaccctg gtcgcgcgct
    14221 atgtgccagc ttcccgctgg ttaacgcgcc aatagccgcg ccgagcggga tcgccgcata
    14281 gagaaaacca atctgtgccg ccgacatctg ccagttgtca gccagcgccg gatacagtac
    14341 ccgcaccgcg ctcgccatcg tcaataaacc acccagcagc gcaatccctc ccaccagcgg
    14401 gctggcgagc agaaaacgaa atcctgccag taatgatttc aacggatgct cacgcggctg
    14461 aggtggcggt ggcaacgccg gaaggcttaa taacggcagc aaggtaataa acgtgcccgc
    14521 cgccgccagc ccgtagttcc aggctacgcc gccggtcgcc agcaataaac cgccaatcat
    14581 gggcgaaatc accgacccca ggcgcacggt caacatggtg atcgccccgg cctgcattaa
    14641 gttttcacgc cctaccagtg caggtgtcgc cgccagtagc gccgtaacgc caagtgatgc
    14701 gaaaaaacca tcccataaac caagtaaata gattgccaac aatgacggct ccggcagcag
    14761 tgcattaagg cacagtccaa tgaagccaat gccacaggtg ccgcgcgcca gcaaaatcac
    14821 ttttttgcgt tcatagcgat ccgccagcac accgccgacc atcaggccaa caaacatcgc
    14881 gccgccggtc agcgtcaccg aaagccccac ctgccaggta gaatgcgtca tcatctggat
    14941 ctgcaccggc accgcgacgc cgagcaaacc cagagacaca attgagataa aacgagcgag
    15001 gaatactgcg cgaaacgccg ggtgcgtttt caacaggctg aggttaagca gccaggattg
    15061 tttattcatt acaatgcctt gccatcaaat attttaaata cctattctta ggctgcacat
    15121 gctaacatat ccaaataaga tcgataacga taattaattt cattatcatg gaagttcgta
    15181 tgtctggttc tgttgccgtg acacgcgcca ttgccgtgcc cggattgctg ttcttactga
    15241 ttatcgcgac ggcattaagc ctgctcattg gggcaaaatc actccccgct tccgtagtgc
    15301 tggaggccat ctccggcacc tgccagagcg ccgactgcac catcgtgctc gacgcgcgac
    15361 tgccgcgcac ccttgccggt ttactggccg gcggtgcgct tgggcttgca ggggcgctga
    15421 tgcaaaccct cacccgaaac ccacttgccg accccggctt gcttggcgtg aacgccggag
    15481 ccagctttgc cattgtgctg ggtgcggcgc tgtttggtta ctcttccgca caggaacaac
    15541 tggcgatggc cttcgccggg gcgctggtgg cctcattgat tgttgccttt accggcagcc
    15601 agggcggcgg gcagttaagt ccggtgcgtt taaccctggc gggcgtggca ctggcggcgg
    15661 tgctggaagg gctgaccagc ggcatcgccc tgcttaatcc tgacgtctac gatcagttgc
    15721 gtttctggca agccggttcg ctggatattc gcaatctaca taccttaaaa gtggtgctga
    15781 tcccggtgct gatcgccgga gcaaccgcgc tattactgag ccgcgcgctg aacagtttaa
    15841 gcctcggcag tgacaccgcg acggcgctgg gtagtcgcgt ggcgcgcaca cagttgattg
    15901 gtctgctggc gattaccgtg ctttgtggta gtgcgacggc ggtagttggc ccgattgcct
    15961 ttattggcct gatgatgccg catatggcac gttggttggt gggtgccgat catcgctggt
    16021 cgctgcccgt cacgctactt gccacccctg ccctgctgct gtttgccgat atcatcgggc
    16081 gggtgattgt gcccggcgag ttgcgcgttt ctgtggtcag tgcctttatt ggcgcaccgg
    16141 tgctgatctt cctcgtgcga cgtaaaacgc gaggtggcgc atgatttacg tctctcgccg
    16201 attaatcatc acctgtttgc tgctaattac cgcctgtgtg gttgcaggta tctggggatt
    16261 acgcagcggt accgtcacgc tggaaacctc gcaggtattc gccgcgctga tgggcgatgc
    16321 gccgcgcagt atgacgatgg tggtcaccga atggcgttta ccacgcgtgc tgatggcgct
    16381 gttaattggc gcggctctgg gcgtcagcgg cgcgattttt cagtcgctga tgcgtaaccc
    16441 gctcggcagc cctgacgtaa tgggctttaa caccggggcg tggagcggcg tgctggtggc
    16501 gatggtgctg tttggtcagg acctgacggc tatcgccctg gcagcaatgg tgggcggcat
    16561 tgtcacctcg ctgctggtct ggctgctcgc ctggcgtaac ggcatcgaca cctttcggtt
    16621 gattattatc ggcatcggcg ttcgcgccat gctggtggcc tttaatacct ggctgttgct
    16681 gaaagcgtct ttagaaacgg cgctaacagc aggtttgtgg aatgccggat cgctcaacgg
    16741 cctgacgtgg gcaaaaacct cgccttccgc acccatcatt atattgatgc tcattgccgc
    16801 cgccttactg gtacgccgga tgcgcttgct ggaaatgggc gatgataccg cgtgtgcgtt
    16861 gggcgtcagc gtcgaacgtt cgcggctgtt aatgatgctg gtggcagtgg tgctcaccgc
    16921 tgcggcaacg gcgcttgccg ggccgatttc ctttattgct ttagtcgcgc cgcacattgc
    16981 ccgacgcatt agcggcaccg ctcgctgggg gctgacccag gcggcgctgt gcggggcgct
    17041 gttactgctg gcggccgatc tctgcgccca acaactgttt atgccgtatc aacttccggt
    17101 tggcgtcgtt accgtcagcc tcggcggtat ttaccttatc gtcttgttaa ttcaggagtc
    17161 tcgcaaaaaa tgaccgaatc agtagcccgt ttgcgtggcg aacagttaac cctcggatat
    17221 ggcaaatata ccgttgcgga aaatctgact gtagaaattc ctgatggtca cttcacggca
    17281 attatcgggc caaatggctg cggtaaatcc acgttactgc gtaccttaag ccgcctgatg
    17341 acgcctgctc acgggcatgt ctggctggat ggcgagcaca ttcaacatta cgccagtaaa
    17401 gaggttgcac gccggattgg cctgttggcg caaaacgcca caacgccggg agatatcacc
    17461 gtgcaggagc tggtggcgcg tgggcgttac ccgcatcaac cgctgtttac tcgctggcgc
    17521 aaagaagatg aagaagcggt aacgaaagcg atgcaggcca cgggaatcac tcatctggca
    17581 gatcaaagcg tggataccct ttctggcgga caacgccagc gagcgtggat agcaatggtg
    17641 ctggcccagg aaacggcaat tatgctgctc gatgaaccaa cgacctggct ggatatcagt
    17701 catcagattg atctcctgga actgttaagc gaactgaacc gcgagaaagg ctataccctg
    17761 gcggcggtgc tgcacgatct taatcaggcc tgtcgttacg ccagccattt gattgcattg
    17821 cgggaaggga aaattgttgc tcagggagcg ccgaaggaga ttgtcactgc tgaattgatc
    17881 gagcgcattt atggtctgcg ctgcatgatt attgacgatc cggtagccgg aactccgctc
    17941 gtggtgccgc tcggacgaac ggcaccttca actgccaaga tttaaactaa gtgctcttcc
    18001 atcacggcat cctgttttct ggatgccatc gcatagcgca acagcacgcc accacaagcc
    18061 accatcccgc cgatcatcgc agaaaggatc acaataatcg ccttacccgg accgtctttt
    18121 ttcactggca atgacgggct taactgatat ttaaacggcg taaaattcac atcgttgata
    18181 tttgcttttg ttaattgctc gacaagatac tgacgattac gtaattcacc gttcagttcc
    18241 gcaacgtcag taaccgcttt ttcaatttcc agtttgcgtt caataccgtc tgcgccgaga
    18301 gaaatagaga aatcggggtc atctttaacg gcctgaccat tactgtaaac gggcttttta
    18361 attcctgccg cgttggcaat gtcgagtgaa taattgaggc gctgaatgtt tgcatcaagt
    18421 tgatttttca ttttaatgcg gtcctgagcc agtttttctt tttcaaactg ggttttaagc
    18481 tccagtctat tgcgctcatt ttctatcgac tctttcacca ccagagcaga gatataatcg
    18541 atatacccgc tcaaaacggt ttgcgcctct tcattggttg gcgcggtaaa acttagcgtc
    18601 caggaggtat aaagtgacgg ttcatctttt ttcttactgg cattgtcatc aaccgctttc
    18661 attttttcgc tcaatgcgac aattgcgcga tgcaaatcca gttcgtcgat tttcgcctct
    18721 tttaattggt ccatcacata aggtgttgag cgcaggtact cttccagcaa gctaaccgac
    18781 tgaaacttct tgataaacag gttaaatgct tctgtacgat caattttgat atccagatcc
    18841 agcactcgaa gcttagtgaa tgttttctcc agctcttgcc actgaacagg ttctggaggc
    18901 gtgacaaccg ccgcgctggt ccatttttgc ggcaggatga aagagatcag caagcctgcg
    18961 caggcaaacg caaaaatgac cgccatgacc gtttttttgg cccgccataa aactgcgatt
    19021 agattaagta aatcaatttc attattactg ggcgacgcca gaggatactc aggaaaatga
    19081 gcgtcacttc cctgtttaat attcagtgat gacatggggt ataacctgaa aatgagtcca
    19141 ataggggaaa tctccacggc gctcacttta ccag
//
LOCUS       DACBVI010000086        18866 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.086, whole genome shotgun sequence.
ACCESSION   DACBVI010000086 DACBVI010000000
VERSION     DACBVI010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.086"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            114..449
                     /gene="eutS"
                     /locus_tag="IU325_14435"
     CDS             114..449
                     /gene="eutS"
                     /locus_tag="IU325_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HAP5810203.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            462..941
                     /gene="eutP"
                     /locus_tag="IU325_14440"
     CDS             462..941
                     /gene="eutP"
                     /locus_tag="IU325_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HAP5810204.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGDYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            916..1617
                     /gene="eutQ"
                     /locus_tag="IU325_14445"
     CDS             916..1617
                     /gene="eutQ"
                     /locus_tag="IU325_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HAP5810205.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESTPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            1614..2417
                     /gene="eutT"
                     /locus_tag="IU325_14450"
     CDS             1614..2417
                     /gene="eutT"
                     /locus_tag="IU325_14450"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HAP5810206.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGD
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            2414..3430
                     /gene="pta"
                     /locus_tag="IU325_14455"
     CDS             2414..3430
                     /gene="pta"
                     /locus_tag="IU325_14455"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP5810207.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWQAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPQLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            3469..3762
                     /gene="eutM"
                     /locus_tag="IU325_14460"
     CDS             3469..3762
                     /gene="eutM"
                     /locus_tag="IU325_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HAP5810208.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            3869..4156
                     /gene="eutN"
                     /locus_tag="IU325_14465"
     CDS             3869..4156
                     /gene="eutN"
                     /locus_tag="IU325_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HAP5810209.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIIDEVVSGGQVIFHK"
     gene            4168..5571
                     /locus_tag="IU325_14470"
     CDS             4168..5571
                     /locus_tag="IU325_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAP5810210.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     IAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAGKIA
                     AAIGLKVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            5582..6418
                     /gene="eutJ"
                     /locus_tag="IU325_14475"
     CDS             5582..6418
                     /gene="eutJ"
                     /locus_tag="IU325_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HAP5810211.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGIY
                     AK"
     gene            6408..7595
                     /gene="eutG"
                     /locus_tag="IU325_14480"
     CDS             6408..7595
                     /gene="eutG"
                     /locus_tag="IU325_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HAP5810212.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGINKRLADVGATTGHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            8111..9337
                     /gene="eutH"
                     /locus_tag="IU325_14485"
     CDS             8111..9337
                     /gene="eutH"
                     /locus_tag="IU325_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HAP5810213.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVLIVAVLVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATAAKTEVEAQS"
     gene            9334..10737
                     /gene="eutA"
                     /locus_tag="IU325_14490"
     CDS             9334..10737
                     /gene="eutA"
                     /locus_tag="IU325_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HAP5810214.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMTLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLDPNSDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            10749..12110
                     /locus_tag="IU325_14495"
     CDS             10749..12110
                     /locus_tag="IU325_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HAP5810215.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            12131..13018
                     /gene="eutC"
                     /locus_tag="IU325_14500"
     CDS             12131..13018
                     /gene="eutC"
                     /locus_tag="IU325_14500"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HAP5810216.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTTCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            13028..13687
                     /gene="eutL"
                     /locus_tag="IU325_14505"
     CDS             13028..13687
                     /gene="eutL"
                     /locus_tag="IU325_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HAP5810217.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            13700..14200
                     /gene="eutK"
                     /locus_tag="IU325_14510"
     CDS             13700..14200
                     /gene="eutK"
                     /locus_tag="IU325_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HAP5810218.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVVESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            14246..15298
                     /gene="eutR"
                     /locus_tag="IU325_14515"
     CDS             14246..15298
                     /gene="eutR"
                     /locus_tag="IU325_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HAP5810219.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSMTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(15304..16203)
                     /gene="hemF"
                     /locus_tag="IU325_14520"
     CDS             complement(15304..16203)
                     /gene="hemF"
                     /locus_tag="IU325_14520"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HAP5810220.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLSAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEDDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTNAYLPIVE
                     RRKTMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYHPEDGSPEAALSEFIKVRDWV"
     gene            complement(16207..17076)
                     /gene="amiA"
                     /locus_tag="IU325_14525"
     CDS             complement(16207..17076)
                     /gene="amiA"
                     /locus_tag="IU325_14525"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HAP5810221.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSRKR"
     gene            17290..17715
                     /locus_tag="IU325_14530"
     CDS             17290..17715
                     /locus_tag="IU325_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase YpeA"
                     /protein_id="HAP5810222.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            17702..18151
                     /locus_tag="IU325_14535"
     CDS             17702..18151
                     /locus_tag="IU325_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 family protein"
                     /protein_id="HAP5810223.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRIWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            18212..18787
                     /locus_tag="IU325_14540"
     CDS             18212..18787
                     /locus_tag="IU325_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HAP5810224.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSNIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
BASE COUNT         4167 a         4958 c         5574 g         4167 t
ORIGIN      
        1 tctctccatt cttagtgatc tacctcacct tttaaacgta cttgccgaat tttgttattt
       61 actctgacga aaaattgtca cgatacacga aagtttttca caggcggcga ctcatggata
      121 aagaacgcat cattcaggaa tttgtgcccg gcaaacaggt cacgctggcg catctcatcg
      181 cgcatcccgg cgaggaactg gcgaagaaaa tcggcgttcc ggatgctggc gcaatcggca
      241 ttatgacgct aactcctggc gaaacggcga tgatcgccgg tgatttagca ttgaaagccg
      301 ctgatgtgca tatcggtttt ctcgaccgct tcagcggtgc attggtgatt tatggctcgg
      361 tgggtgcagt agaagaagcg ttatcacaaa ccgtcagcgg tttagggcgt ttattaaatt
      421 atacgctttg tgaaatgacc aaaagttaat acgaggcatt catgaaacga attgcgtttg
      481 tgggtaccgt cggggcagga aaaacaacgt tatttaatgc gttgcaggga gattataccc
      541 tcgccagaaa aacgcaggcc gtagaattta atgataaggg tgatattgat acgccgggtg
      601 aatattttag ccatccccgc tggtatcacg ccttaattac cacgctacag gatgtggaca
      661 tgctgatcta tgtccacggc gcgaatgacc cggaaagtcg gttgcctgcc gggttgctgg
      721 atattggcgt cagcaaacgg caaatcgccg tcatcagtaa aacggacatg ccagatgctg
      781 acgtcgccgc aacgcgaaaa ttattgcttg agacgggctt cgaagagccg attttcgagc
      841 taaacagcca cgacccgcaa agcgtgcagc aactggtgga ttatctggca tctctcacca
      901 aacaggagga agcaggtgaa aaaactcatc acagcgaatg atattcgtga agcacacgcg
      961 cgcggcgaac aggcaatgtc cgtggttctg cgcgccagca ttattacccc ggaagcccgt
     1021 gaagtggcag atctactggg ttttaccatc actgaatgtg atgaatcgac cccggtaacg
     1081 gcgtccgttc ccgccagcgt gccagccgat aaaaccgaaa gccagcgcat ccgcgaaacc
     1141 atcattgccc agctgccgga aggccagttt accgaaagcc tggtcgcgca gctgatggaa
     1201 aaagtgatga aggaaaaaca gtcgctggag cagggcgcaa tgcagccgag cttcaaatcg
     1261 gtgaccggca aaggcggtat caaagttatt gatggcagca gcgtcaagtt tggtcgtttc
     1321 gacggcgcac agccacactg cgtcggatta acggatctgg tcaccggaga cgacggcagc
     1381 agcatggccg ctggttttat gcagtgggag aacgccttct tcccgtggac cctgaactat
     1441 gacgaaatcg acatggtact ggaaggggaa ctgcacgttc gccacgaagg cgaaaccatg
     1501 atcgccaaag caggcgacgt gatgtttatc ccgaaaggct ccagcatcga atttggtacg
     1561 acatccagcg tgaaattcct gtatgtcgcc tggccggcta actggcaatc cctatgaaag
     1621 atttcatcac cgaagcatgg ctaagagcga accatacgct cagcgaaggc gcagagatcc
     1681 atctccctgc ggacagtcgc ctgacgccct ctgcccggga gttgctggaa agccgccatc
     1741 tgcgcatcaa gtttattgac gagcagggcc gcctgtttgt tgacgatgaa cagcagcagc
     1801 cgcagcccgt tcatgggctg accagtagcg atgaacatcc gcaggcgtgc tgcgaactgt
     1861 gccgccagcc ggtggcgaaa aagccggata cgctgactca cctgtcggcg gaaaaaatgg
     1921 tcgccaaaag cgatccgcgc ctgggttttc gcgcggttct cgacagcacc attgcgctgg
     1981 cagtgtggct gcaaattgaa ctggcggaac cgtggcagcc gtggctggcg gatatccgtt
     2041 cgcgtcttgg caacattatg cgtgccgatg cgctggggga accgctgggc gatcaagcga
     2101 ttgtcgggct ttctgacgaa gatctgcatc ggctttctca tcagccgctg cgctatctcg
     2161 accacgatca tctggtgccg gaagccagcc acgggcgcga tgcggcgttg ctcaatctgc
     2221 tacgcaccaa agtgcgggaa accgaaaccg tagcggcgca ggtctttatc acccgcagct
     2281 ttgaagtatt acgcccggac atcttgcagg cgctgaaccg cctttccagc acggtctacg
     2341 tgatgatgat cctgagcgta acgaagccgc cgctaacggt gaagcagatc caacaacgac
     2401 tgggggaaac gcaatgatta ttgaacgttg tcgtgaactg gcgttgcgag cgcccgccag
     2461 agtggttttt ccggatgcgt tagatcagcg tgtgctgaaa gcggcgcaat atttacatca
     2521 acaaggtctg gcaacgccca ttctggtcgc taatccgttt gaacttcgtc agtttgcgct
     2581 cagtcatggc gtagcgatgg acgggctaca ggtgattgat ccgcatggca acctcgcaat
     2641 gcgggaagaa tttgctcatc gctggctggc ccgcgcgggc gaaaaaacgc cgccggatgc
     2701 gctggaaaaa cttaccgacc cgctgatgtt cgccgcagca atggtcagcg ccggtaaagc
     2761 ggatgtctgt atcgcgggca acctctcttc cacggcgaat gtgctgcgtg ccggattacg
     2821 cattatcggc ttgcagccag gctgtaaaac gctctcatcc attttcctga tgctgccaca
     2881 gtacagcggt ccggcgttgg gttttgccga ttgcagcgtg gtgccacagc cgacggcggc
     2941 gcagttggcg gatatcgcgc ttgccagcgc cgaaacctgg caggccatca ccggagaaga
     3001 accgcgcgtg gcgatgttgt cgttttccag taacggcagc gcccgtcacc cctgcgttgc
     3061 caacgtccag caggcgacag aaatcgtccg tgagcgcgcg ccgcagttgg tagtcgatgg
     3121 cgagttgcag tttgacgccg ccttcgtgcc ggaagtggcg gcgcaaaaag cgcctgccag
     3181 cccgctacaa ggcaaggcca atgtgatggt ttttccgtcg ctggaagccg gaaatattgg
     3241 ctacaaaatc gcacaacgac tcggcggata tcgtgccgtc gggccattga tacaaggact
     3301 tgccgcgccg atgcacgatc tctctcgtgg ttgtagcgtg caggaaatta tcgagctggc
     3361 gctggtggca gctgtgccgc gtcagacaga agtgaaccgc gaaagcagtt tacaaacact
     3421 ggttgaatga acggtcccgt tctggaccct gataaagagg aaagcacaat ggaagcatta
     3481 ggaatgatcg aaacccgggg cctggttgca ctgattgagg cttctgacgc gatggtgaaa
     3541 gctgcgcgcg tcaagctggt tggtgttaag cagattggcg gcggcctgtg tactgccatg
     3601 gttcgtggtg atgttgcagc ctgtaaagcc gcgaccgacg caggtgctgc agcggcacag
     3661 cgtatcggcg agctggtatc tgtacacgtt atcccgcgtc cgcacggcga cctggaagaa
     3721 gtcttcccga tcggcctgaa aggcgatagc agcaacctgt aatcaccctg tgtgacgtcg
     3781 attcgggcgt cacgctctct cctttttcag tttaccttcc gcggagggta gggggagctt
     3841 ttacctgaaa tacgccacgg aggcgggtat gaaactggca gtcgtcactg gacaaattgt
     3901 ttgtaccgta cgccatcacg gactggcgca tgacaaattg ctgatggtgg aaatgattga
     3961 tccacaaggt aatcctgacg ggcaatgcgc cgtcgccatc gacaatattg gcgcgggaac
     4021 cggggagtgg gtgttgctgg tgagtggcag ttccgcccgc caggcgcata aaagcgaaac
     4081 gtcaccggtc gatctgtgcg tgattggcat catcgatgag gtggtgtctg gcggtcaggt
     4141 aattttccac aaataaggca gaacatcatg aatcaacagg atattgaaca ggtggtgaaa
     4201 gcggtactgc tgaaaatgca aagcagtgat acgccgcccg ccgccgttca tgagatgggc
     4261 gttttcgcgt ccctggatga cgccgttgcg gcagccaaaa ttgcccagca agggttaaag
     4321 agcgtggcaa tgcgccagtt agccattgct gccattcgtg aagcaggcga aaaacacgcc
     4381 agagatttag cggaacttgc cgtcagtgaa accggcatgg gccgcgtcga agataaattt
     4441 gccaaaaacg tcgctcaggc gcgcggcaca ccgggcgttg agtgcctctc tccgcaggtg
     4501 ctgactggcg acaacggcct gactttaatt gaaaacgcgc cctggggcgt ggtggcttcg
     4561 gtaactccgt ccaccaaccc ggcggcaacc gtaattaata acgccatcag cctgattgcc
     4621 gcgggcaaca gcgtcatttt tgccccgcat ccggcggcga aaaaagtctc ccagcgggcg
     4681 attacgctgc tcaaccaggc gattgttgcc gcaggcgggc cggaaaactt actggttact
     4741 gtggcaaatc cggatatcga aaccgcgcaa cgtttgttca agtttccggg catcggcctg
     4801 ctggtggtga ccggcggcga agcggtagta gaagcggcgc gtaaacacac caataaacgt
     4861 ctgattgccg caggcgctgg caacccgccg gtagtggtgg atgaaaccgc tgacctcgcc
     4921 cgtgctgctc agtccatcgt caaaggcgcg tctttcgata acaacatcat ttgtgccgac
     4981 gaaaaggtac tgattgttgt ggatagcgta gccgatgaac tgatgcgtct gatggaaggc
     5041 cagcacgcgg tgaaactgac cgcagagcag gcgcagcagc tgcaaccggt gttgctgaaa
     5101 aatatcgacg agcgcggaaa aggcaccgtc agccgtgact gggttggtcg cgacgcaggc
     5161 aaaatcgcgg cggcaatcgg ccttaaagtt ccgcaagaaa cgcgcctgct gtttgtggaa
     5221 accaccgcag aacatccgtt tgccgtgact gaactgatga tgccggtgtt gcccgtcgtg
     5281 cgcgtcgcca acgtggcgga tgccattgcg ctggcggtga aactggaagg cggttgccac
     5341 cacacggcgg caatgcactc gcgcaatatc gagaacatga accagatggc gaacgccatt
     5401 gataccagca ttttcgttaa gaacggaccg tgcattgccg ggctggggct gggcggggaa
     5461 ggctggacca ccatgaccat caccacgcca accggtgaag gggtaaccag cgcgcgtacg
     5521 tttgtccgtc tgcgtcgctg tgtattagtt gatgcgtttc gcattgttta aggagtaacc
     5581 gatggcgcac gacgaacaat ggctcacccc gcgtctgcaa acggcggcaa cgctgtgtaa
     5641 ccagacgcct gccgcaacgg aatctccgct gtggctgggg gtggatctgg gcacctgcga
     5701 tgtggtgtcg atggttgtcg accgcgacgg tcagccggtg gcggtatgcc tcgactgggc
     5761 tgatgtggtc cgcgacggca tcgtctggga tttcttcggt gcggtcacca ttgttcgtcg
     5821 ccatctcgac accctcgagc agcaattcgg tcgccgtttt agccatgcgg cgacctcttt
     5881 tcctccaggt acggacccgc gtatttccat caacgtgctg gaatctgccg gactggaagt
     5941 gagccatgta ctggatgagc caaccgcagt cgcggatctg ctgcaactgg acaacgccgg
     6001 tgtagtggat atcggcggtg gcactactgg tattgccatc gtgaaaaagg gcaaggtgac
     6061 gtactcggcg gatgaagcga ccggcggaca tcacatctct ctgactctcg ccggaaatcg
     6121 tcgtatttcg ctggaagagg cagagcagta caagcgcggt cacggtgaag agatttggcc
     6181 cgcggtgaaa ccggtgtacg aaaaaatggc ggacattgtt gcccggcata ttgaagggca
     6241 gggcattact gatttatggc tggcgggcgg ctcttgtatg caaccgggcg tggcggaact
     6301 gtttcgcaaa caattcccgg cgttacaggt gcatttaccg cagcacagtt tgtttatgac
     6361 cccgctggcg atcgccagta gcgggcgaga gaaagcggag gggatctatg caaaatgaat
     6421 tgcagaccgc gctctttcag gcgttcgata ccctgaatct gcaacgggta aaaacattta
     6481 gcgttccacc ggtgacgctt tgcggtccgg gctcggtgag cagttgcgga cagcaagcgc
     6541 aaacgcgtgg gctgaaacat ctgttcgtga tggcagacag ctttttgcat caggcaggga
     6601 tgaccgccgg gctgacgcgc agcctggccg ttaaaggtat cgccatgacg ctctggccat
     6661 gtccggtggg cgaaccgtgc atcaccgacg tgtgtgcagc cgtggcacag ttgcgtgaat
     6721 caggttgtga cggggtgatc gcgtttggcg gcggctcggt gctggatgca gcaaaagccg
     6781 tggcgttgct ggtgacgaac ccggatagca cgctggcaga gatgtcagaa accagcgttc
     6841 tgcaaccgcg cctgccgttg attgccattc caacgaccgc cggaaccggc tctgaaacca
     6901 ccaacgtaac ggtgattatc gacgcggtga gcgggcgcaa gcaggtgtta gcccatgcct
     6961 cgctgatgcc ggatgtggcg attctcgacg ctgcgctgac cgaaggcgtg ccgtcgcatg
     7021 tcacggcgat gaccggtatc gacgcgttaa cccatgctat tgaagcgtac agcgcgctga
     7081 acgccacacc gtttaccgac agtctggcga ttggtgccat tgcgatgatt ggcaaatcgc
     7141 tgccgaaagc ggtgggctac ggtcacgacc ttgccgcccg cgagagcatg ttgctggctt
     7201 catgtatggc gggaatggcg ttttccagtg cggggcttgg gttgtgccac gcgatggcgc
     7261 atcagccagg ggcggcgctg catattccgc acggtctggc gaacgccatg ctactgccaa
     7321 cggtgatgga gtttaaccgg atggtctgtc gtgaacgctt tagtcagatt ggtcgggcgc
     7381 tgcgaactaa aaaatccgac gatcgtgacg ctattaacgc ggtaagtgag ctgattgcgg
     7441 aagtcgggat caacaagcgg ctggcagacg tgggcgcaac caccggacat tacggcgcat
     7501 gggcgcaggc cgcgctggaa gatatttgtc tgcgcagcaa cccgcgtacc gccagcctgg
     7561 agcagattgt cggcctgtac gcagcggcac aataaatgcc ggatgcgacg cttgctgcgt
     7621 cttatccggc ctacaggatt gcacatgtag ggcggataag gcgttcacgc cgcatccgcc
     7681 agtggcgcgg tgcaattgcc ggatgcgacg cttgccgcgt cttatccggc ctacaggatt
     7741 gcacatgtag ggcggataag gcgttcacgc cgcatccgcc agtggcgcgg tgcaattgcc
     7801 ggatgcgacg cttgccgcgt ctgatccggc ctacaggatt gcacatgtag ggcggataag
     7861 gcgttcacgc cgcatccgcc agtggcgcgg tgcaattgcc ggatgcaacg cttgctgctc
     7921 ttatccggcc tacaggattg cacatgtagg gcggataagg cgtttacgcc gcatccgcca
     7981 gtggcgcggt gcaattgctg gatgcgacgc ttgctgcgtc ttatccggcc tacaggattg
     8041 cacatgtagg gcggataagg cgtttacgcc gcatccgcca ataaataaca actaacaggg
     8101 agtaaaggcg atgggaatta acgaaatcat catgtacatc atgatgttct ttatgctgat
     8161 agctgccgta gacaggatcc tgtcgcagtt cggcggttct gctcgtttcc tcggtaagtt
     8221 cggtaaaagt atcgaaggat ccggcgggca gtttgaagaa ggctttatgg cgatgggcgc
     8281 actgggcctg gcgatggttg gtatgaccgc gctggcacct gttctggccc acgtactcgg
     8341 gccggtgatt atcccggttt acgaaatgct cggcgcaaac ccgtcaatgt tcgccggaac
     8401 actgctggcg tgcgatatgg gcggcttctt cctcgccaaa gagctggcgg gcggcgacgt
     8461 agcagcgtgg ctatactctg gattaattct cgggtcgatg atgggtccaa cgattgtgtt
     8521 ttccattccg gtggcgctcg gcattatcga accttctgac cgtcgttatc tggcgctcgg
     8581 cgtgctggcg ggcattgtga ccattccgat tggctgtatt gccggtggtc tggtggctat
     8641 gtattccggt gtgcagatca acggtcagcc agtggaattc acctttgccc tgatcctgat
     8701 gaacatgatc ccggtactta tcgtcgcggt actggtggcg ctggggctga aattcatccc
     8761 ggaaaaaatg atcaacggct tccagatctt cgccaaattc ctcgttgcat tgatcaccct
     8821 cggtcttgcc gctgcggtag tgaaattcct gctcggttgg gaactgatcc ccggtctgga
     8881 tccgatcttc atggctccag gcgacaaacc cggtgaagtg atgcgcgcca ttgaagttat
     8941 cggttctatc tcctgcgtgc tgctgggggc gtatccgatg gtgctgctgc tgactcgctg
     9001 gtttgaaaaa ccgctgatga gcgtcggtaa ggtgctgaat atgaacaaca tcgcggcagc
     9061 tggcatggtc gcaacccttg ccaacaatat cccgatgttc ggcatgatga agcagatgga
     9121 tacccgcggc aaagtgatca actgtgcctt cgccgtttcc gctgctttcg ccctgggtga
     9181 ccacttaggc ttcgccgctg ccaacatgaa cgccatgatc ttcccgatga ttgtcggcaa
     9241 gttgatcggc ggcgtaacgg cgattggcgt ggcgatgatg ctggtgccaa aagaagacgc
     9301 gaccgcggcc aaaaccgaag tggaggcaca atcgtgaaca ctcgccagct actgagcgtc
     9361 ggtatcgata tcggcaccac caccacccag gtgatcttct cccacctgga gctggttaac
     9421 cgtgcggcgg tgtcgcaggt gccgcgctac gaattcatca aacgcgaaat tagctggcaa
     9481 agcccggtgt tctttacccc tgtcgataaa cagggtggtt taaaagaagc ggaactgaaa
     9541 accttaatcc tggagcaata tcaggctgcg ggcattgcgc cggaaagtgt tgattctggt
     9601 gccatcatca tcaccggtga aagcgcgaaa acccgcaacg ctcgcccggc ggtgatgacg
     9661 ctctcccagt cgctgggcga ttttgtcgtc gccagcgccg ggccgcatct cgaatccgtg
     9721 atcgccggtc acggtgccgg ggcgcaaacc ctttctgaac aacggctgtg tcgggtactg
     9781 aatatcgaca tcggcggtgg caccgcgaac tacgccctgt tcgatgccgg aaaaatcagc
     9841 ggcactgcct gcctcaacgt cggtggtcgc ctgctggaaa ccgacagcca ggggcgcgtg
     9901 gtttacgctc ataaaccggg gcagatgatt gtggatgagt gcttcggcgc aggcaccgac
     9961 gcccgttcgc tgaccggcgc gcagctggtg caggttaccc ggcggatggc agagctgatt
    10021 gtcgaagtga ttgacggaac gctctcgccg ctcgcgcagg cattaatgca aaccggtttg
    10081 ctgcccgcag gtgttacgcc cgaaatcatt acgctttctg gcggcgtggg cgaatgttat
    10141 cgccaccagc ccgccgatcc gttctgtttt gccgatatcg gcccgcttct ggcaacggcg
    10201 ctgcatgacc atccgcgcct gcgtgagatg aacgtgcagt ttccagcgca aaccgtacgc
    10261 gccacggtga ttggcgcggg tgcacatacc ctttcgctct ctggcagcac aatctggctg
    10321 gagggcgtac aactgccgct gcgcaatttg ccggtggcga tcccaattga tgaaacggat
    10381 ctggtgagtg cctggcaaca ggcgctgatt cagctggatc ttgatcccaa ttctgacgcg
    10441 tacgtgctgg cgcttcccgc ctcgctgcct gtgcgttacg ccgcggtact gacggtcatc
    10501 aacgcgctgg tcgatttcgt cgcgcgtttt ccgaatccgc atcccctgct ggtggtggcc
    10561 gggcaggact ttggtaaagc tctgggcatg ttgttgcgcc cacagctaca acaactcccg
    10621 ttggcagtca ttgacgaagt gattgtccgc gcgggggact atatcgacat tggtacgcct
    10681 ctttttggcg gatcggttgt gccggtgacg gtgaaatcac tcgcatttcc ttcctgaggg
    10741 aacgacttat gaaactaaag accacattgt tcggcaatgt atatcagttt aaggatgtaa
    10801 aagaggtgct ggctaaagcc aacgaactgc gttcggggga tgtgctggcg ggcgttgcag
    10861 cggcaagctc acaggagcgc gtggcggcaa agcaggtgtt gtcggaaatg accgtagcgg
    10921 acatccgcaa taatccggtg attgcctatg aagatgactg cgtgacgcgg ctgattcagg
    10981 acgacgttaa cgaaacggcc tacaaccaga ttaaaaactg gagcatcagc gaactgcgtg
    11041 agtatgtgct gagcgatgaa accagcgtgg acgacattgc ctttacccgc aaagggctga
    11101 cctcggaagt ggtcgcggcg gtagcgaaga tttgctccaa cgcggacctg atctacggcg
    11161 cgaagaaaat gccggtgatc aaaaaggcca ataccaccat cggtattccg ggcaccttca
    11221 gcgcccgctt gcagccaaac gacacccgtg acgacgtgca aagtatcgcc gcgcaaatct
    11281 acgaagggct ttctttcggg gtgggcgatg cggtgatcgg cgttaacccg gtgactgacg
    11341 acgtggaaaa cttaagccgc gtgttggaca ccatctatgg cgtgatcgac aaattcaaca
    11401 ttccgaccca gggctgcgtg ctggcgcacg tcaccaccca gattgaagcg atccgtcgtg
    11461 gcgcaccagg cgggctgatt ttccagagta tctgcggcag cgaaaaaggg ctgaaagagt
    11521 ttggcgtgga actggcgatg ctcgacgaag cgcgcgcagt gggcgcggag ttcaaccgta
    11581 tcgccgggga aaactgcctc tacttcgaaa ccggacaagg ctctgcgcta tccgctggcg
    11641 ctaacttcgg cgcagaccag gtgacgatgg aagcgcgtaa ctacgggctg gcgcgtcatt
    11701 acgatccgtt tatcgtcaac accgtggtcg gctttattgg gccggagtat ctctacaacg
    11761 accgccagat tatccgtgcg ggcttagaag atcactttat gggcaagctg agcggcatct
    11821 ctatgggctg tgactgctgc tacaccaacc acgctgacgc tgaccagaac ctcaacgaaa
    11881 acctgatgat cctgctcgcc accgcaggct gcaactacat catggggatg ccgctgggcg
    11941 atgacatcat gctcaactat cagaccaccg cattccacga cactgccact gtgcgtcagt
    12001 tactcaacct gcgcccgtca ccggagtttg aacgctggct ggaaagcatg ggcattatgg
    12061 caaacggtcg cctgaccaaa cgggcgggcg atccgtcact gttcttctga tgacgcggag
    12121 ataacacatc atggatcaaa aacagattga agaaattgta cgcagcgtga tggcgtcaat
    12181 gggacaagcg gcccccgcgc cgtcagaagc aaagtgcgcc accaccacct gtgcggcacc
    12241 ggtgacctcg gaaagctgcg cgctggattt aggctctgcg gaagcaaaag cgtggattgg
    12301 cgttgaaaac ccgcatcgcg cagacgtatt aacagaactg cgtcgcagca ccgtggcccg
    12361 cgtttgtacc ggtcgtgccg gtccgcgtcc gcgtacccag gcgttgctgc gtttcctggc
    12421 cgatcactct cgttcgaaag ataccgtact gaaagaagtg ccggaagagt gggtgaaagc
    12481 gcagggcttg ctggaagtcc gctctgaaat tagcgacaaa aatctctacc tgactcgtcc
    12541 ggatatgggc cgccgcctgt gtgcagaagc tgttgaagcg ctgaaagcgc agtgtgttgc
    12601 caatccggac gtgcaggtcg ttatttctga tggcctgtca actgatgcga tcaccgtgaa
    12661 ctacgaagag atcctgccac cgctgatggc gggcctgaaa caggccgggc tgaaagtcgg
    12721 tacgccattc tttgttcgtt atggtcgcgt gaagattgaa gatcagatcg gtgagatcct
    12781 cggcgcgaaa gtagtgatcc tactggtggg ggaacgcccg ggattagggc agtcagaaag
    12841 cctctcctgc tacgccgttt actcgccgcg tatggcaacc accgttgagg ccgaccgtac
    12901 ctgtatctct aacattcacc agggcggcac gccgccggtt gaagccgcag ccgtcattgt
    12961 ggatttggcg aaacgtatgc tggagcagaa agcatccggc atcaacatga cccgataagg
    13021 aggcatcatg ccagctttag atttgattcg accgtcggtc accgccatgc gggtgattgc
    13081 ctctgttaac gccgaatttg cgcgtgaact gaaattgccg ccgcatattc gtagccttgg
    13141 gctgatttct gctgattccg atgacgtcac ctacattgcg gctgacgaag cgaccaagca
    13201 ggcgatggtc gaagtggtgt atggccgctc gctgtatgcg ggagctgcac acggaccgtc
    13261 accgactgcc ggtgaagtgc tgattatgct cggtgggcca aacccggcgg aagtgcgtgc
    13321 tggtctggat gcgatggttg cgcatattga aaatggcgcg gctttccagt gggctaatga
    13381 cgcagaaaac acagctttcc tggcacatgt agtttcgcgt accggttctt atctctcatc
    13441 aaccgccggg atcacgcttg gcgatccgat ggcgtatctg gtggcaccgc cgctggaagc
    13501 gacctacggc attgatgcgg cgttgaaatc tgccgacgtg cagctggtga cctatgtccc
    13561 gccgccgtct gaaaccaact actcggcagc atttttaacc ggtagccagg ccgcgtgtaa
    13621 agcagcctgt aacgctttta ccgatgcagt gctggaaatc gcgcgtaatc caatccagcg
    13681 tgcgtaacgg aggttgccga tgatcaatgc actgggattg ctggaagtgg acggcatggt
    13741 cgccgcgata gatgcagcgg atgccatgct caaagcagct aacgttcgtc tgctcagtca
    13801 cgaagtgctt gaccctggtc gcctaacgct ggtggtggaa ggcgatctgg cggcgtgtcg
    13861 tgcggcgctg gacgcaggtt gtgctgccgc gatgcgtacc gggcgtgtca tcagccgtaa
    13921 ggagatcggt cggccagacg atgacaccca gtggctggtt actggcttta accgccagcc
    13981 gaagcaaccc gtaaaggaac ccgacgcgcc agttatcgtc gtggaatctg ctgacgagtt
    14041 gttggcgctg ttaacatcag tacgtcaggg aatgacggca ggagaagtgg ctgcccactt
    14101 tggctggccg ctggaaaaag ccagaaatgc gctcgaacag ctcttttctg ccgggacgtt
    14161 acgtaaacgc agtagtcgtt atcgtctcaa gccccattaa cctgtcggag gtgccgggtg
    14221 tcctacaaca cccggcatta acatcatgaa aaagacccgt acagccaatt tgcaccatct
    14281 ttatcatgaa cccttacccg aaaacctgaa gctcacgccg aaggtcgaag tggataatgt
    14341 tcatcaacga cagacaacgg atgtctatga acatgctttg acgattaccg cctggcagca
    14401 gatttacgat cagctgcatc cgggcaagtt tcatggtgaa tttacggaaa ttctactcga
    14461 tgatattcag gtttttcgtg aatacaccgg tctggcgctg cgtcagtcgt gcctggtctg
    14521 gccgaattcg ttctggtttg gcattccggc gacgcgcgga gagcagggat ttatcggttc
    14581 gcaatgtctg ggaagcgcag aaatagcgac gcgccctggt ggaaccgagt ttgaattaag
    14641 tacgccggat gattacacga tccttggcgt agtgctttct gaagatgtca tcacccgtca
    14701 ggctaacttt ttgcataatc cggatcgggt attacatatg ctgcgtagcc agtcggcgtt
    14761 ggaagtgaaa gagcagcata aagccgcgct gtggggcttt gtccaacagg cgctggcgac
    14821 attttgcgag aacccggaaa atcttcatca gcctgctgtg cgtaaagtgc tgggggataa
    14881 tttgctaatg gcgatggggg cgatgctgga agaagcgcag ccaatggtga cggcggaaag
    14941 catcagtcat cagagttacc gtcggttgct ttcccgcgcc cgtgaatatg tgctggaaaa
    15001 catgtccgaa ccggtgacgg tgctggactt gtgtaatcaa ctgcatgtca gccgccgcac
    15061 gctacaaaac gcgtttcacg ctattttagg cattggccca aatgcgtggc tgaaacgcat
    15121 tcgcctgaac gccgtgcgcc gcgaactgat aagcccgtgg tcgcaaagta tgacggtaaa
    15181 agacgccgcc atgcagtggg gattctggca tctggggcaa ttcgccacgg attaccagca
    15241 gctgtttgcc gagaagccgt cactgacgct gcatcagcgg atgcgggaat gggggtgagg
    15301 gagttacacc caatccctga ccttaataaa ctcactcaac gccgcttccg ggctgccatc
    15361 ttctggatga taatcgtact cccagcgtac cagcggcggc attgacatca ggatagactc
    15421 ggtgcgcccg ccagtttgca aaccaaacag cgtgccacga tcccagacca gattgaactc
    15481 gacataacga ccgcgacgat ataactggaa attgcgctcg cgctcgccgt aggccatcgt
    15541 tttacgtcgc tctacaattg gtaaataagc gttggtgtag cctttgccta ccgcctgcat
    15601 aaaggcaaaa cagtggtcga agtctggcgt gttcaggtca tcaaagaaca ggccgccaat
    15661 gccgcgttgt tcgttacgat gtttgaggta gaagtattcg tcgcaccact ttttgtaacg
    15721 cggataaacg tcttcgccaa atggcaggca caggtcacgg gcggtgcggt gccagtgaat
    15781 agcatcgtct tcaaagccat agaaaggggt taagtcgaag ccgccgccaa accaccagac
    15841 gggttcggca cccggttttt cggcaataaa aaagcgcaca ttcgcgtggc tggtgggaac
    15901 atacggatta tgcggatgca ctaccagtga aacgcccatc gcctcgaaac tgcgcccggc
    15961 aagttccggg cgatgagcgg tggcggaagc aggcatcgcc tcaccgtgga catgtgaaaa
    16021 gttgacaccc gcctgttcga aaacaccacc attacgcaac acccggctac gtccgccgcc
    16081 gccagcttcg cgctgccagc tatcttcgac aaactctgcg ccatcgacgg cgctcagctg
    16141 ctgacaaatt gtatcctgaa ggttgagcag gaactgttta acctggtgtg cgtcgggttt
    16201 catcacttac cgctttctcg agtgtgcttt ctggttgtca aaccagtgga aataactgat
    16261 cacgccttcg gcaatcgcag tggcgatttt ctggcgaaat gccgccgtgc ctaacagccg
    16321 ctcttcttcc gggttggtga taaacgaggt ttccaccagc accgaaggaa ccgacggcga
    16381 tttcaacacc acaaacgcgg cttgttcggt gttgcggctg tgcagtttat gcaccggctt
    16441 aatcttcttc agaatatgcg agccgagcgt caggctgttt ttaatagtat cggtttgcac
    16501 cagatcaaac agcacttgct gcaataggtg atccttgtca gtcgcctttt taccagcaac
    16561 ttcatcggcg cggttttcgc gttcagacag gtatttcgcc attgcgctac ttgcaccgcg
    16621 gttagagagg gcaaataccg aagcaccggc agctttcggg ttggtaaagc cgtcggcatg
    16681 aattgacata aacagatctg cgccatgttt atgggcgatt tcaacgcgat cgtaaagtgg
    16741 gataaacgta tcgccagaac gcgttaaacg cgcatcaatc ccatgattac gcaaaatgga
    16801 acggacgttt ttagcaatcg ccagcaccac atgtttttct ttcgaaccgt tgcgtccgat
    16861 cgctccggta tcgatcccac cgtggcctgg atcgagaaca acgacacgtt tgccgccaga
    16921 ttttttggct ttcggcttgc tgtgtccgtt gctggttttt aaaggttcgt ctttggcgat
    16981 ggcttgcgac attcctgaca acgtcagggc agccaaaccg gctttcagca cctggcggcg
    17041 cgaagtgagt gtttttagtg gtttaaaagt gctcatacgg cctgagttgt aataataaag
    17101 ttccagatgt tatatcgtat cgcgtaatcc gttacgaccg tttgctgatg agaattgttt
    17161 tacttttcat ttcaatagtt gacaactccc cattatgcca ctttttcaac cgattttcgt
    17221 cagatattgg ctaattcgac cgttcacgcc ataatcagcc ttttaacccg caaaaaggta
    17281 acgaatacca tggagatacg cgtatttcgc caggaagatt tcgaagaggt catcaccctt
    17341 tgggagcgtt gcgacttgct gcgtccgtgg aacgatccgg aaatggacat cgagcgtaag
    17401 atgaaccatg acgtcagttt gtttctggtc gctgaggtga acggtgaagt ggtcggaacg
    17461 gtgatgggcg gttatgacgg gcatcgcggg tctgcttatt atcttggcgt gcatccagag
    17521 tttcgtgggc gtgggattgc taatgcgttg cttaatcggc tggagaaaaa gctgattgct
    17581 cgcggctgtc cgaaaattca gatcaacgtg ccggaagata acgacatggt gctcggaatg
    17641 tatgaacgcc tgggatatga acacgccgac gtgctgagtc tgggtaagcg tttgattgaa
    17701 gatgaagagt actgagtttc atcctgtcca ttatgatgcg cacggtcgcc tgcgtttacc
    17761 cttgctcttc tggcttgtgc tattgcttca ggcgcgaacc tgggtgctgt ttgtcattgc
    17821 tggtgcgtcg cgcgagcagg gtactgcgct gctaaatctg ttttatcccg atcacgataa
    17881 tttctggctg gggttgattc ccggcattcc ggcggtgctg gcgtttttgc tgagtggtcg
    17941 gcgggcttca tttccccgta tctggcatgt gctctatttt ctgttgctgt tggcgcaggt
    18001 ggttttactt tgctggcaac cgtggctgtg gctgaacggt gaatccgtta gcggtatcgg
    18061 gctggcgctg gtagtggcgg atattgtggc gttaatctgg ctgctgacca atcgacgttt
    18121 acgcgcttgt tttaatgaag aaaaagaata acggcacttt ttggtgaatt tgcactccaa
    18181 gcaacgttat tgaataacca aaggaagtga catgaaatcg ctgcgtttaa tgttatgcgc
    18241 tatgccgttg atgctgaccg gctgttccac gatgtcgtca gttaactggt ctgccgctaa
    18301 cccgtggaac tggtttgggt cgtccaccaa agtgagcgag cagggcgttg gtgaattaac
    18361 ggcgtccaca ccactgcaag aacaagccat tgccgatgcg cttgatggcg attatcgcct
    18421 gcgcagcgga atgaaaaccg cgaacggcaa cgtggtgcgc ttttttgaag tgatgaaagg
    18481 cgacaacgtg gcgatggtga ttaacggcga tcagggtacg atcagccgca ttgatgtgct
    18541 ggatagcaat attcctgctg acaccggtgt taaaatcggt acaccgttta gcgaccttta
    18601 cagcaaagca tttggcaatt gccaaaaagc tgatggtgat gataatcgtg ccgtcgaatg
    18661 taaagccgaa ggcagtcaac atattagcta ccagttcagc ggggaatgga gtggtcctga
    18721 agggttaatg ccttcggacg ataccctgaa aaactggaaa gtcagtaaaa ttatctggcg
    18781 gcgttaattt gcgtctgaac aaaccgccgc cgcgaaaaaa cacgtaaaat agcgctcaac
    18841 aatgccacgg attgcgtggc attctt
//
LOCUS       DACBVI010000087        18600 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.087, whole genome shotgun sequence.
ACCESSION   DACBVI010000087 DACBVI010000000
VERSION     DACBVI010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.087"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..905
                     /locus_tag="IU325_14545"
     CDS             <1..905
                     /locus_tag="IU325_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HAP5810225.1"
                     /translation="PWVNALPPTPRHASPEQLEKTVRYLTQTVHPRSADNIDNLNRSA
                     EYIKEVFVSSGARVTAQDVPIAGGPYKNIVADYGPADGPLIIIGAHYDSVSSYENDQL
                     TYTPGADDNASGVAGLLELARLLQQQAPKTGVQLVAYASEEPPFFRSDEMGSAVHAAS
                     LERPVRLMIALEMIGYYDSAPGSQDYPYPAMSWLYPDRGDFIAVVGRMQDINAVRQVK
                     AALLSSRDLSVYSMNAPGFIPGIDFSDHLNYWQHDIPAVMITDTAFYRNKQYHLPGDT
                     ADRLNYQKMAQVVDGVITLLYNSK"
     gene            1008..1511
                     /locus_tag="IU325_14550"
     CDS             1008..1511
                     /locus_tag="IU325_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HAP5810226.1"
                     /translation="MGMIGYFAEIDSDKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPANNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLEA
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1579..3036)
                     /gene="nuoN"
                     /locus_tag="IU325_14555"
     CDS             complement(1579..3036)
                     /gene="nuoN"
                     /locus_tag="IU325_14555"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HAP5810227.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3043..4572)
                     /gene="nuoM"
                     /locus_tag="IU325_14560"
     CDS             complement(3043..4572)
                     /gene="nuoM"
                     /locus_tag="IU325_14560"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HAP5810228.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4738..6579)
                     /gene="nuoL"
                     /locus_tag="IU325_14565"
     CDS             complement(4738..6579)
                     /gene="nuoL"
                     /locus_tag="IU325_14565"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HAP5810229.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARSHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     ITHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6576..6878)
                     /gene="nuoK"
                     /locus_tag="IU325_14570"
     CDS             complement(6576..6878)
                     /gene="nuoK"
                     /locus_tag="IU325_14570"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HAP5810230.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6875..7429)
                     /gene="nuoJ"
                     /locus_tag="IU325_14575"
     CDS             complement(6875..7429)
                     /gene="nuoJ"
                     /locus_tag="IU325_14575"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HAP5810231.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7441..7983)
                     /gene="nuoI"
                     /locus_tag="IU325_14580"
     CDS             complement(7441..7983)
                     /gene="nuoI"
                     /locus_tag="IU325_14580"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HAP5810232.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7998..8975)
                     /gene="nuoH"
                     /locus_tag="IU325_14585"
     CDS             complement(7998..8975)
                     /gene="nuoH"
                     /locus_tag="IU325_14585"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HAP5810233.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8972..11698)
                     /gene="nuoG"
                     /locus_tag="IU325_14590"
     CDS             complement(8972..11698)
                     /gene="nuoG"
                     /locus_tag="IU325_14590"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HAP5810234.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11751..13088)
                     /gene="nuoF"
                     /locus_tag="IU325_14595"
     CDS             complement(11751..13088)
                     /gene="nuoF"
                     /locus_tag="IU325_14595"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HAP5810235.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13085..13585)
                     /gene="nuoE"
                     /locus_tag="IU325_14600"
     CDS             complement(13085..13585)
                     /gene="nuoE"
                     /locus_tag="IU325_14600"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HAP5810236.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13588..15390)
                     /gene="nuoC"
                     /locus_tag="IU325_14605"
     CDS             complement(13588..15390)
                     /gene="nuoC"
                     /locus_tag="IU325_14605"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HAP5810237.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15484..16146)
                     /locus_tag="IU325_14610"
     CDS             complement(15484..16146)
                     /locus_tag="IU325_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017490251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HAP5810238.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16162..16605)
                     /gene="nuoA"
                     /locus_tag="IU325_14615"
     CDS             complement(16162..16605)
                     /gene="nuoA"
                     /locus_tag="IU325_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HAP5810239.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17235..18173)
                     /gene="lrhA"
                     /locus_tag="IU325_14620"
     CDS             complement(17235..18173)
                     /gene="lrhA"
                     /locus_tag="IU325_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HAP5810240.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4621 a         5162 c         4827 g         3990 t
ORIGIN      
        1 aaccctgggt caatgcatta ccgccaacgc cccgacacgc aagtcctgag caattagaaa
       61 aaacagttcg ttatcttaca caaactgtgc atccacgtag tgccgacaat attgataatc
      121 tgaataggtc cgcagagtat ataaaagagg tctttgtcag tagcggtgcc agagttaccg
      181 cgcaggacgt ccccattgcg ggtggcccct acaaaaacat tgttgctgat tatggtcctg
      241 ccgatggacc gctgattatt attggtgcgc actatgactc tgtcagtagt tatgaaaatg
      301 atcaattgac ctatacgccg ggcgcggatg ataacgccag cggcgtggca ggattactcg
      361 aactggcacg tttgttacag cagcaagcac cgaaaactgg cgtgcagctg gtcgcctatg
      421 cgtcggaaga accgcccttc tttcgtagcg atgaaatggg gagcgcggta catgcagctt
      481 cgcttgagcg tccagtgaga ttaatgatag cgctggagat gattggctat tacgactctg
      541 cgcctggtag tcaggattac ccttacccgg caatgtcctg gctttatccc gatcggggag
      601 actttattgc cgtggtcggc agaatgcagg atatcaacgc cgttcgtcag gtaaaagcgg
      661 cattgttgtc atcccgggat ttatctgttt attctatgaa tgccccaggg tttattcccg
      721 gtattgattt ctctgaccac ctgaattatt ggcaacacga tattcccgca gtaatgatta
      781 ctgacaccgc tttttatcgt aataaacaat accacttgcc cggtgatacc gcagacagat
      841 tgaattatca gaaaatggct caggtagtgg atggggttat aactttgtta tacaacagta
      901 aataataaaa atttctcgag gcgggtccag aaaaagcgac atgtacattt tattttccct
      961 cgtctgtttt aagatgcgaa acatgaaatt caacggagga aaataaaatg ggaatgattg
     1021 gctattttgc agaaattgac tcagataaga ttaaccaact gcttgagtct actgaaaaac
     1081 ccttaatgga caatattcat gacacgcttt caggattacg ccgactggat attgataaac
     1141 gctgggattt tttacacttt gggctgacag gcacctctgc ttttgatccc gcgaataatg
     1201 atccgctgag tcgtgccgtg cttggtgaac acagcctcga agatggcata gatggcttcc
     1261 tgggattgac ctggaatcag gagttagccg cgacaatcga ccgactggaa gctctggatc
     1321 gaagtgagct gcgtaaacag ttttccatta aacgactcaa tgaaatggag atttaccctg
     1381 gtgtgacttt ttcagaagag ttagaggggc aactctttgc ttcgatcatg cttgatatgg
     1441 aaaagctgat tagtgcgtat cgacgtatgc tgcgacaagg gaatcatgcg ctgacggtta
     1501 ttgttgggtg atggggtcag ggcaccaaaa aaaaaccgcc gaacatgtcg gcggctttct
     1561 gacttacaaa ctgactgctt acatcagcgg catggccaac cgcacaatgc taatcagcgg
     1621 ttgtggccat acacccagca ccagtaccaa cagtgcagag atcagcacca cgataccgcc
     1681 cgcgctgtac tgccagtttg aaggtgcatc gcgacccggt tgttccgggg cgtgaagata
     1741 caagctcacc gccacgcgca ggtagtagta gaggccgatt gccgaaccga caaccacggc
     1801 acccaccagc caccacaagt gtgcctggac accgaccgcc agcacgtaga acttaccaat
     1861 aaagcccagc gtcatcggaa taccggccag agacagcatc atcaccgtca tcaccgccgc
     1921 gaggatcggg cgatgccaga acagaccgcg gtaagagaac agggaatcag catccgggcc
     1981 acgatacggg ctggacatca ggctgaccac gccgaacgcg ccgaggctgc tgaacagata
     2041 accggccagg taaaccccta ccgcttccat cgacatctcg ccggtttgca gcgcaatcag
     2101 cgccaccagc agatagccga ggtgagagat agatgagtaa ccgagcaaac gtttgatatt
     2161 ggtctggctc agcgccatca ggttaccgaa gatgatggag gcaaaggcga taatcgccag
     2221 caccacccga atcgcttcgc tgtcacccac cggtgcgtag aggaacagac gcatcaccac
     2281 accgaagata gcgattttgc tcgccgtcgc caggaaagtg gaaaccggcg caggcgcgcc
     2341 ctggtatacg tctggcgtcc acaggtggaa cggcaccaga gagagtttga agccgaggcc
     2401 aacaatcatc aggccgaaac ctgccagcaa cagcggctcg ttgagcatac cgtcgccgag
     2461 gtttttaccc agcgcgacaa acgacaggtc gccagactgc gcatacacca gcgccatacc
     2521 gaacagcagg aaagaagacg ctgcggcaga gagaatggtg tatttgatac ttgcttccag
     2581 cgaacgtttc tggcggaaag cgtaaccgac caggccaaac agcggcaaag agatcagttc
     2641 aataccgagg aacaaagacg ccagatggtt ggcattcgcc agcaggatcc cgcccagcgc
     2701 ggcaattaac accaacaggt agaactcgtc cttgttgtcg ttatagcctt caagccacgg
     2761 gtaggcgaaa gtacaggtgg cgaggctcgc caacaatacc agcccggtgt aaagcatggc
     2821 gaaaccatca acgcgcatca gtggcgtaac gtccatagcg cccgcctggc cgacaaacca
     2881 gagcgaaacc agcgccgcgt taagcccaat aaccgagagc gtagcgttga ggaaatgatt
     2941 gcgtcgccac gcaatggaga gcatcacaac caccaccgtc aagccgacga tcagcaacgg
     3001 tagcagtgcg atcaggtttt gtggagttat tgtcatggcg atttacggcc ttgtagtagt
     3061 aacggaatta acaaaccact gctggatatt gccaatcgcg gagtgcgagg tatccagaat
     3121 cggctgcgga tagaagccca gcagtaccag cagcaccacc agcaacagga tcataaacag
     3181 ctcacgcagc gacatccctg gcagttcctg gctggcaatc tggcttttcg ctttaccgaa
     3241 gtaagcgcga tgtaacatcg ccagcgaata aacagaggca aagaccagcc caaaggtaga
     3301 gataacggta atcaccggga caacctggaa gctgccgaac agaatcataa attcgccgac
     3361 gaagttaccg gtgccaggca tcccaagcgt tgccaccgca aagaacagcg acagtgctgg
     3421 cagccatttc atcttgctcc acagaccgcc catcatgcgc atgtcgcggg tatggatacg
     3481 ttcataaagc tgaccgcaga gaataaacag acccgccgct gacaagccgt gcgcaatcat
     3541 ctggattacc gcgccctggt aggccaactg gctgccagtg tagatagcaa tcagcacgaa
     3601 gcccatgtgg gaaaccgagg tgtaggcgat cagacgtttg atatcggtct gggcgaaggc
     3661 catccacgca ccgtagaaga tgccgataac acccagccac atggcgattg gcgcgaactc
     3721 tgccgacgcg ttcgggaaca gcggtaggga gaaacgcagc agaccgtaag cggcggtttt
     3781 cagcaagatc cccgcgaggt ccacggaacc ggcggttgga gcctgtgagt gcgcatccgg
     3841 cagccagcca tgcagcggaa ccaccggcat tttcaccgca aatgcgatga agaagcccag
     3901 catcaacaga tattccacac cactggacat tggcgtattc aacagctctt catagttgaa
     3961 ggtccagacg ccggtcgcat tgtagtgaac aaaaaccagc gccaggatgg cgatcaacat
     4021 caccagacca ctcgcctggg tgtaaatgaa gaacttggtt gccgccgtga tacgcgtttt
     4081 accgtcagag gctttatgcc cccacagtgc gatcaggaag tacatcggca ccagcatcat
     4141 ttcccagaag aagaagaaca ggaacatgtc gatggcaagg aacacgccga taacgccgcc
     4201 caggatccac atcaggttga ggtggaagaa gccctgatat ttttcgatct ctttccacga
     4261 acagagtacc gccagcacac cgagcagacc agtcagcacg accatcagca gcgatagccc
     4321 gtcaatggcg agatggatag agataccaaa acgcgggatc cacggcatgt cgaattcaga
     4381 ctgccactgc ggaattccgg cggattgcgt cagtgaataa ccgccctgca accacagttg
     4441 cagcgacagc gccagcgtca atcccatggt gatcagcgcg atccagcgcg gcaccttgac
     4501 gccaaagcgt tcggtctgcc agcacaggaa gccgccgata aagggaatta atattagcca
     4561 gggtagtaac atggcgatct ttattccttg taaaagtccc gtcaggaccg gattttcaac
     4621 gaattctcac gacagaaaat tctcttcgga ttgtggttgt agtgtcggat gcgccgcgaa
     4681 ggcggcttat ccgacctacg ttcttatatg cccaggggca agcccacaat ccttaactca
     4741 acgcagtacc atcaacagtg ccagcaccac gaccgcaccg atgctcatgg atgccacata
     4801 ccagcgcaga taaccgttct cgcttaacag cagaccttta cctgcaaagc gggaaaggac
     4861 agccgggatg ttcatcattg agttcagcgg atcgcgtttc agcaaccagg caatacccag
     4921 gaacggcttg acgaacactt tgtcgtacag ccagtcaaag ccccaggcgt tgtaccacca
     4981 ggtgcccagc agacggcccg gcgcactgtt ggcgatggaa gtcaccagag tacgtttacc
     5041 cagccacagc caggctgcca gcagaatgcc gaccaccgcg accacgccag aggtaatttc
     5101 cagggtcaac atgctgccgt gcgccagctc cgtcgtttgc gggagcacgc cctgcagcgg
     5161 cggtacaatc agtgcgccaa cgaaggtgga aaggatcagc agcacaatca gcggcaggct
     5221 gtgagttatc cctttcacgg cgtgagcgtg aatttgttct tttccgtgga agacgatgaa
     5281 aatcatacgg aaggtgtaga gcgaggtcat aaacgcaccg accagaccag ccaccatcag
     5341 attgatatga ccattcgcca tcgcacccgc gaggatctca tccttactga agaagcccgc
     5401 agtgaccagc ggcagtgccg acagtgctgc gccgcccacc aggaagcaga gataaaccag
     5461 cggaatagat ttacgcagac cgcccatctt gaagatgttc tgttcgtgat ggcaggccag
     5521 aatgacggaa ccggatgcca ggaacagcag cgctttaaag aacgcgtgag tcatcaagtg
     5581 gaaaatcgcc gcatcccatg cctgcacgcc aagcgcgagg aacatgtagc caatctggct
     5641 catggtagag taagcgagaa cacgtttgat atcggtctgt accagcgcgg caaaaccggc
     5701 cagcagcagc gtaaccgccc caacaatgcc caccagatgc agaacttccg gcgtcatcag
     5761 gaacaggccg tggctacggg cgatcaggta gacacccgcg gttaccatgg ttgcggcgtg
     5821 gatcagcgcg gagacaggcg tcgggcccgc catcgcgtcg gcaagccatg tctgcaacgg
     5881 caactgcgca gatttaccga ccgcaccgcc cagcagcatc agcgtcgccc acatcagcat
     5941 gttattgccg tcagcaaagt gcgctggtgc cagttccacc atttcgcgga agttcagggt
     6001 gcccagttcg ttgtaaagaa tgaacagcgc gaaagcgagg aagacgtcac ccacacgggt
     6061 cacgacgaat gccttcattg ccgctgcgcc attcttcgga tcggtgtaat agaaaccgat
     6121 cagcagatag gagcacaggc ccacgccttc ccagccgagg tacatcagca gcaggttgtc
     6181 ggcaagcacc agaactacca tgctggcgat gaacaggttg gtgtaagcga agaagcgaga
     6241 gtagccctct tcaccgcgca tataccagga ggcgtacatg tgaataagga aacccacacc
     6301 agtgaccacc gagagcatgg tcagcgacag gccgtccagc accaggttaa aaccgatgtt
     6361 aaagtcgcct accgacatcc acgtccacag cggctggctg tatgcctgct caccgttagc
     6421 gaagaagtca acgccgataa aggcagttac cagcgccgcc aggcccacag agccaacgcc
     6481 gacgatcgcc gagacgtttt cagaccagcg cccacgggag aacgccagca ggacgaagcc
     6541 aatcaatggc aaaataatgg ttaaggcaag catgttcatc cgcgcatctc acttactgaa
     6601 tcaatgttca ggttctggcg acgacggtga agttgcagca gcagcgctag gccgatactc
     6661 gcttctgccg ccgcgaggct gatggcgaga atgtacatca cctgaccgtc ggtctggccc
     6721 cagtagcttc cggcgaccac aaaggccagc gcggaggcgt taatcatgat ttccagacca
     6781 atcagcataa acagcagatt gcgacggata accagaccag ttaagccaag aatgaataag
     6841 attgccgcga ggatcagtcc atgttgtaag gggatcatgc gtgctcctcc gtttttcttt
     6901 tcgcgctgtc gtctttacga ttgctcagca cttcacccgc acgctcttca cgaccgacgt
     6961 ggaaggccac aaccagaccc gcgagcagca gcatagaagc cagttccacc gccagtacgt
     7021 aaggcccgaa cagcgtaata cccactgctt tagcactgat tggcgtaccg tcgataccct
     7081 gatcgttaac accgaggatg gcgtaaacaa tcaccaccag catgatggcc gacaaaattg
     7141 ccggaccaat ccacacctgc ggtttcagcc actggcgttc ctgttcaatt tctgaaccgc
     7201 ccaggttgag catcatcacc acgaacacga acagcaccat aatggcaccc gcgtagacga
     7261 taatttccag cgcaccggcg aagtaagcgc ccagtgagaa gaacacccca gagatcgcca
     7321 gcagcgaaat aatcaggtac agcagtgcgt gtaccggatt ggtatgggtg atcactcgca
     7381 aggtcgcaag tatggctatc aggccacaga tataaaaagc gaactccatt gcccctctcc
     7441 ttacggtaac aggctcttga cgtcgatagg cttggcttcg ttctctgctt cgcccttatc
     7501 tttgccgtcg attgccatac ctgccatccg gtagaagtta tattccgggt atttgcccgg
     7561 accggagatc agcagatcct ctttctcgta aaccagatcc tggcgcttgt attcccccat
     7621 ttcgaaatcc ggggttaact gaatcgccgt ggtcggacag gcttcttcgc acagaccaca
     7681 gaaaatgcag cgtgagaagt tgatgcggaa aaattccgga taccagcgac cgtctttggt
     7741 ttctgctttt tgcagcgaga tacagccgac cgggcaggct accgcgcaga ggttacaggc
     7801 tacgcaacgc tcttcgccgt ccgggtcgcg ggtcagaacg atacgaccac gataacgggg
     7861 cggcagatag accggttctt ccgggtacat tcgcgtttcg cgtttggcga acgcgtgcag
     7921 gccgatcatc cagatactac gaacctgggt gccgaatcct actaacaatt cttttaaggt
     7981 catggtctta ttgcccctta ttgcgcctgc cagagaatga cagccgccgt taccagcaag
     8041 ttgatcagcg tcagcggcag gcagattttc cagccgaagg acattacctg gtcataacgc
     8101 ggacgcggta acgacgcacg aatcaaaatg aacatcatca taaagaacgc ggttttcagc
     8161 gcgaaccaga tgaatggcgg taacaacggg ccttgccagc caccgaagaa cagcgtcacc
     8221 atcagtgcag agatggtcac aatcccgatg tattcgccca cgaagaacag accgaacttc
     8281 atgccggaat attcaatgtg gtaaccatcc gccagttcct gctcggcttc cggctggtca
     8341 aacgggtgac ggtgacacac cgccacgccc gcgatggcaa aggtaataaa accaaagaat
     8401 tgcgggatga cgttccacac atgcgcctgg ctgttgacga tgtcggtcat gttgaatgaa
     8461 ccggcctgcg ccaccacgcc catcagggaa agcccgagga acacttcgta gctcagagtc
     8521 tgcgcagaag cacgcatcgc acccagcagc gagtatttgt tgttacttga ccagcccgca
     8581 aacagcaccg cgtagaccgc cagaccggcc atcatcagga agaacaaaat cccgatgttc
     8641 aggtcggcaa ccacccaacc tgggctgact ggcacaatcg caaaggccag cagcagcgag
     8701 gtaaaggcaa tcatcggtgc cagggtaaag atgacgcgat ccgagaattt cgggatccag
     8761 tcttctttaa agaacatctt gatcatgtcc gcgaccagct ggagcgaacc gccccagcca
     8821 acacggttag gtccgtaacg gttctggaac agacccagca ggcgacgttc gccaaagctc
     8881 atgaatgccc cacaggtgac aaccaccagc aggatcacca ccgctttgag gatggtcagc
     8941 aggatctcaa tcagttccgg tgatatccaa ctcattgttg tgcctccttg agatcctcaa
     9001 gatgcgcgcc cgccagcacc ggagcaatgc cggacatacc catcggcaag cccacctgcc
     9061 ctgccgtcag cccttcggcg atttcaaccg gcagcgtgac cgtgttgcca tcgtaactaa
     9121 aggagacgcg tgtacccgcg ttcacaccca acttcgcggc atccgctggg ttgagtttga
     9181 tgtacggctg cggcatacgg ctctggaaga ccggagcacg ctgtgacaat tcatcgctgc
     9241 caaacaggtg gtaatacggc gcgatacgcc atttcccgtc ctgcggctgg aagcgtgccg
     9301 gtacgctggt gaagtaatcc agaccgtttt cgctggtttc aaacagacgc acgcccggat
     9361 cgccaaagcg cagtttgccg ccaacttcgt cctggaattt gttccacgcc tgcggggagt
     9421 tccagcctgg cgcccaggca aacggcactt gcgaacggtg cgcggtcggc tggttgttac
     9481 cttccatcga gaaggtgaac atggtgtcaa tatcctgcgg ctgacgcggc tcatgaacgc
     9541 tgatattggc gcgcatggcg gtacgaccgc tgtaacggtg cggttcacgg gccagtttct
     9601 gcccacgaat acggaatgtc gcatccggcg cagcatcttt gatacccgcc agttccggga
     9661 ttttcgccac aacagcgtca atcacatggt cgagctgcgt ccagtccact tcacggctca
     9721 gcaaggtgct atgcagcgag tgtaaccagc gccagctttc cagcatgaca gttttgctgt
     9781 cgtaataagc cggatcgtaa acctggaaga aacgttgggc gcgaccttca ttattaatta
     9841 ccgtaccgtc gctttcagca aagctggcag cagaaagtac cagatgggcg ttttccataa
     9901 tcgctgtgcg ttgatggtca accaccatca ccagcggcgc tttagccagc gcagcattca
     9961 cgcgggtagc ggaagcgtga cgatgcagat cgttttccag caccaccacc gcgtcggcgc
    10021 gtccggtttc cagttcggtc aacgcttctt caagcgaacc gccccccata atgcccagcc
    10081 ccatgctgtt tacagagcga gcaatcatgg tgataccaac gtcagcgccg cgacctttca
    10141 gggctttcgc gacgttagcc gccgcctgaa tcacctctaa gctaccggcg ttcgtcccgg
    10201 agataatcaa cggtttcttc gcaccagcca gtgcctgcac gatgacgtcg attttgcttt
    10261 gcagctcagg ttcaataccg tcaaccgctg gtgcggagtt gtccagcgca tgggcgatgg
    10321 caaaacctaa acgcgcctga tcttcaaccg gtgcgcggta agtccacgcc gcgatatcat
    10381 ccagacgggt gtcatcaacg ttggtaacaa acagcggatg cttcgcacgt tgaccgatgt
    10441 tgaggattgc cgcaatctgc cagtcagcca ctttctgtgc tgccgccatt tcgcgcgctt
    10501 tacctttcac cgcctgacga actgccagcg cgacgcgcgc gccggtctgg gtaacgtctt
    10561 cgcccagcac cagtaccgca tcgtaagatt cgatttcgcg cagtgccggc gtataaatgc
    10621 cgccttcacg tagcactttc agcgccagtt gcagacgttc ctgctcaccg tgagcgatac
    10681 cggtatagaa gttttcttcg cccaccagtt cacgcagcgc aaagttgctt tctacgctgg
    10741 cacgcggaga accgatgccg atcactttct tcgactggcg cagaatatct gctgcgccct
    10801 gcattgcctg ttcggcattg agggtaatga aatcatcgcc acgacgctgt actggctgac
    10861 gcggacgatc tttcaggttg acgtaaccgt aaccgaaacg accacggtcg cagaggaagt
    10921 agtggtttac cgtaccgttg taacggtttt cgatacgacg cagttcgcca taacgctcgc
    10981 cggggctgat gttacagccg atggaacatt gctggcagat gctcggcgca aactgcatat
    11041 cccatttacg gttgtaacgc tcggagtgcg ttttgtcggt gaatacgccg gtcgggcaaa
    11101 tttcgaccag gttaccggaa aattcgcttt ccagcgtgcc gtcttccggg cgaccgaagt
    11161 agacgttgtc gtgcgcaccg taaacgccca ggtccgtgcc gtcagcgtaa tctttgtagt
    11221 aacgcacgca gcggtagcag gcgatgcagc ggttcatttc gtgagagatg aatggcccca
    11281 aatcctgatt acggtgggta cgtttggtga aacggtagcg acggaagctg tgtccggtca
    11341 tcacggtcat atcctgaaga tggcagttac cgccttcttc acataccgga cagtcgtgcg
    11401 ggtggttggt cattaaccac tcgaccacac tttcacggaa ctgtttcgct tcttcatcgt
    11461 caatggaaat aaaggtgcca tcggaagccg gtgtcataca ggacatcacc aggcgaccac
    11521 gcgtgtcttc cgcgttttgg tattgcttca ccgcacactg gcggcaagca ccgacgcttc
    11581 ccagcgccgg atgccagcaa aagtaaggaa tatcaaggcc cagagacaga caagcttcca
    11641 gcaagttgtc cgctccgttg acctcgtatt ctttgccgtc tacatgaatt gtagccatta
    11701 gcatgcttcc agttttctca gtcagagact gagcgttaat cgaaattcgg ttaccagcgc
    11761 tctttcagca ggttcggctg aatcccatta atcaaatggg tattgctgaa cggctgtttg
    11821 attcccgcct caaattcttc gcggaaatat ttgatggcgc tctgtaacgg ctccactgca
    11881 ccaggtgcgt gggcacagaa agttttaccc gggcctaaga atcgacacag ttgctcaagt
    11941 gtttcgatat cgcccggctg accttcgcca cgctccagcg cacgcagaat tttcacgctc
    12001 cacggcagac cgtcgcggca cggcgtacac cagccacagg actcacgggc gaaaaactct
    12061 tccaggttac gcaccagcga caccatgttg atctcatggt caaccgccat cgccagcgcc
    12121 gtacccagac ggcttcccgc tttaccgata ctttcgaatt ccatcggcag atcaaggtgc
    12181 gcttcggtca ggaagtcggt gccagcgccg cctggctgcc aggctttgaa tttcagaccg
    12241 tcacgcatac caccggcata atcttcgagg atctcgcgtg cggtggtgcc gaatggcagt
    12301 tcccacaggc ccggattttt cacccgaccg gagaagccca tcagcttggt gccagcatct
    12361 ttacttttcg agatgttctg ataccactcc acgccgttag cgaggatcgc cggaacgtta
    12421 cacagggttt cgacgttgtt gacacaggtc ggtttgcccc atacgccgga ggttgccggg
    12481 aaggggggtt tcgagcgtgg gttagcacga cgcccttcca gggagttgat taacgcggtt
    12541 tcttccccgc agatgtagcg ccctgccccg gtatggacga acagttcgaa gtcaaaacct
    12601 gttcccataa tatttttgcc aagcagacct gcttcggtgg cttcggcaat ggcacggcgc
    12661 agattcactg ccgcttcgat atattcgcca cgcaggaaga tgtagccacg gtaagctttc
    12721 agcgcaaacg cggagataag cataccttcc accagcaggt gcggcagttg ctccatcagc
    12781 aggcggtctt tataggtgcc cggctccatt tcatcggcgt tacatagcag gtaacggatg
    12841 ttcatggatt cgtctttcgg catcaggctc catttcaggc cagtcgaaaa gcccgcgccg
    12901 ccgcgccctt tcagaccagc gtcttttacc tgattaacaa tttcgtccgg agacagcccg
    12961 gtcagcgcct tacgcgcgcc ttcgtaaccg tttttgctgc ggtattcatc cagccacact
    13021 ggctgtttgt catcgcgcag acgccaggtc agcggatgcg tttcgggagt acggataatg
    13081 tttttcattt ataccgctcc agcagttcag ggatcgcttc cggggtcaga tgcgcgtgag
    13141 tgtcctcatc gatcatcatg tttggccctt tatcacagtt ccccaggcag caagttggca
    13201 gcagcgtaaa gcggccatca aatgtcgttt gccctggttt gatgttcagc tttttctcga
    13261 gcgccgcctg aataccctga taaccgttga tatgacagac cacgctgtcg caataacgga
    13321 tcacatggcg accaaccggc tggcggaaga tctggctata aaacgttgcc acaccttcga
    13381 cgtcgctcgc cggaataccc agcacatcgg cgatcgcgtg gatcgcgcca tccggcaccc
    13441 agccacgctg cttctgaacg attttcagcg cttcaatgga cgccgcacgc gggtcttcgt
    13501 agtggtgcat ttcgtgctca atcgcttcac gctctgccgc actcagctca aaagcctcgg
    13561 tttgtggttg ttgattctcg tgcataatta gcggtccaca tctgacataa caaaatcgat
    13621 actgcccaga taaacaatca ggtcagacac caggctgccg cggatcgccg ccggaatttg
    13681 ctgcaaatgc gcataactcg gcgtacggat acgggtgcga taactcatgg tgctaccgtc
    13741 gctggtcagg tagtaactgt tgatcccctt ggtcgcctca atcatctgga aagattcatt
    13801 ggctggcatc accggacccc acgacacttg caggaagtgg gtgatcaggg tttcgatatg
    13861 ttgcagcgtg cgctctttcg gcggcggcgt ggtcagcggg tgatccgctt tgaacgggcc
    13921 ttccggcatg ttgttgaggc actgctcaag aatgcgcaga ctctgacgca gctcttccac
    13981 tttaagcatt acgcgggtgt agcagtcaga aacaccacca cccaccggga tttcaaagtc
    14041 gaagttttca tagccagaat acggacgcgc cttacgcacg tcgaagtcga tcccggtagc
    14101 acgcaggccc gcgccggtgg tgccccactc cagcgcctct ttcgcgccat aggcggcaac
    14161 gccctgggaa cgacctttca gaatggtgtt ttgcagcgcc gctttctcgt aagacgccag
    14221 acgtttcggc atccagtcga ggaactcacg cagcaggcga tcccagccac gcggcaggtc
    14281 gtgcgctacg ccgccaatac ggaaccacgc cgggtgcata cggaaacccg tgatcgcttc
    14341 caccagatcg taaattttct gacgatcggt aaaggcgaag aacactggcg tcattgcgcc
    14401 gacgtcctga ataaaggtcg agatatacag cagatggctg ttgatgcgga acagctcaga
    14461 aagcattacg cgaatgacgt taacgcgatc cggcacggtg atcccggcca gtttctctac
    14521 agccagcacg taaggcattt cgttaacgca gccgccgagg tattcgatac ggtcggtata
    14581 cggaatgtag ctatgccagg actggcgctc gcccattttc tccgcaccac ggtggtggta
    14641 gccgatgtct ggtacgcagt cgacaatctc ttcgccatcg agctgcaaaa cgatacggaa
    14701 agccccgtgc gccgacgggt ggttcgggcc gaggttgagg aacatgaagt cctcgttttc
    14761 ggttccgcgc ttcatccccc actcttccgg tttgaaggtc agggcttcca tctccagatc
    14821 ctgtttggct ttggtcagct caaacggcga gaattcggta gcgcgcgccg ggtaatcttt
    14881 acgcagcggg tgacctttcc aggtttgcgg catcatgatg cggcgcaggt tcgggtgacc
    14941 gtcgaaagta atgccaaaca gatcccaggt ctcacgctca taccagttag cgttcgggaa
    15001 cagtttggtg aaggtcggta cgtgcaggtc gttttctgcc agcgccacct tcaacatgat
    15061 gtcgcggtta cgatcgatag aaatcagatg gtagaaaacg gaaaaatccg cggcaggtaa
    15121 cccttcgcgg tgtgtgcgca gacgttcgtc catgccgtgt aagtcaaaca gcatgacgta
    15181 aggtttcggc agtttcttta agaaatcgcc aacttccagt aattgttcac gcttgatcca
    15241 cacaacggga accccggtgc gagtcgcctg aacagtaaag gcatccggcc caaaacggtt
    15301 gcgcagttcg ccaatcaccg gatcatcaag atgatcgcgg gtctgccagg cgggttcttg
    15361 cgcggttaag tcggtcatat tgttcaccat tgcaaatggt ccgtggtgac tgtcggcgca
    15421 gtaagcttcg cgaaatagat ttaggaataa gcgaagaaaa atccctttgc cgacaggcgc
    15481 aaattaaatc tcgtcaggtg tacgcaggtt ggttacggca atgcgttcac cgcgcttgcg
    15541 ttcgcgctct gattgcatat tggcgcgata aacgccctga tcgccaacca cccaggagag
    15601 cggacgacgt tctttgccga tagattcctg caacagcatc agtgcctgca tgtacgcttc
    15661 aggacgcggc gggcagcccg ggatatacac atcaaccggg atgaatttat caacgccctg
    15721 cacaacggaa taaatatcgt acataccgcc agagttggca caggcaccca ttgagataac
    15781 ccattttggc tccagcatct ggtcatacag acgctgaata accggtgcca ttttggtaaa
    15841 gcaggttcct gccaccacca tcaggtcagc ctgacgcggc gaagcacgca atacttctgc
    15901 gccaaaacgc gccacgtcat gcaccgcggt aaacgaagtc accatctcaa cgtaacagca
    15961 ggaaagaccg aagttatacg gccaaattga gtttttacga ccccagttaa ccatgtcatt
    16021 gagcttgccc ataaacacgt ttttgttaac ttcttgctcc agagggtcgg ttacgatctc
    16081 ctgcttttgc agggggtaac ggtcgttctc accgttggga tctatgcggg tgagcgtata
    16141 atccatctta atgcctcgcg gttagcgttg acgattagcg atactgttcg tttccgggtt
    16201 catacgctcg cggcgtgaac gcgcgggcgt ccagtccagc gcgccaatac gcaccagata
    16261 aaccagacct gccagtaaca caaaaataaa aattgcagct tccacaaagc ctacccagcc
    16321 gctttcgcgg atagaggttg accatgcgaa cagatacagc gcttcaacgt cgaagataac
    16381 gaagaacatg gccaccagat aaaacttggc ggacaggcgt aagcgggcgg agccgaccga
    16441 gtcgataccg gattcaaacg gcacgttttt cgacctcgcg cgtgcgcgac cgcctaaaaa
    16501 ccaaccgcct accagcatca ggcaacacag gccaatggca acgataagaa agatagcgaa
    16561 tgcccagtga tgagcgatga cttcagtgga tgttgacata ctcattgctt actcatcaaa
    16621 agtagcgcca gattcactgc tcttcacggc agatggacgc cacatcgatt catggggagg
    16681 aataaaaaaa accttacaat cactgtagaa attcttttat acagctaatt gatgtggtct
    16741 tttactcctt tctataacct tttgtcaact ttaacaaaag tttcttcaca ttagtttaca
    16801 taatatcaac accattagca tttaatgccc tttcacccca gatccttgac gactccagga
    16861 taattagatg ttgttgaatc gtgtccgttg tgaagcaatg gaaaaaatac gggtctattt
    16921 tgacaggaat ttgtgtcgat tcctcccccc aaaagagagt attttcttga tctgtgacac
    16981 gcttttgtca ttccataaca aaaacgcagc aacaaattta cgtatttttt aacatcattg
    17041 tagcaggtga tttttttcag gcgattattt gtgcgttcgg gacgtgaatc tctggtgatt
    17101 gaaaaatgaa cagttttgta tgttctgcac gatacgagga aggcttttta ctaaaaaaaa
    17161 gccgctgggg tttaaaacac ccccagcggc tcgtttttac actattgtct cagaaattat
    17221 ctatggtccg tcgattactc gatatccctt tcaatcaaca atgagtcatc gccttccgga
    17281 gcagacattg ggctgtattg ccacggatta tggtagctct ccatcgcctg atagatcacc
    17341 tgggccagct cattattact ggacgggtca tagcacagca aatattcggt atcgggtagc
    17401 ggcggtaagc catctactcc actcagcaca cgcaggtctg gactcatcat ctcgaccggc
    17461 cttgccgtca cgccaagacc ggcttttact gccgcacgaa ctgccggaag cgtcgaggcg
    17521 acataagcca gtcgccatgg aatatctgct ttattaagcg tcgccagcac catatcgcga
    17581 aacgggctag gatcatccag caatacaaga gggatcggct cgcctttttg caatacgtat
    17641 tccgctgcgc agtaccagtg tgttggcgag gtacgcaggt tcaatgcctt aaacgttgaa
    17701 ggacgatggg tggttaccat caaatccact tcctgggatt ccagcatttc tgccatatag
    17761 gcattacgtt taacgcggac atccagcgct agtttcggat aaaccgaact cacgcgattt
    17821 aacaggaaag gtaagatcgt atcggcagat tcatctgaag ctccgatagt taacacgccc
    17881 tgaagattac tgaacattaa tgatgagcag gcctcatcat taaaacgcag gattttcctg
    17941 gcgtaaccaa gaagttgaat gccatgttca gttaacagtt tgttgcgacc gtgacgagcg
    18001 aacagttctt tcccaacgag ttgttccaga cgctgcattt gctgacttac ggcggactga
    18061 gtacgacaca cagcggcagc tgcggcagca aaagtgttca gatcggcaac agcaacaaat
    18121 gttctcagca gatcgaggtc gaggttaatt atcggacgat ttgcacttat catatattat
    18181 cacttactgg cggctcatac tgagctggtt aatgctgtgc acacacaaac aagcaattcc
    18241 atttgtaatg tgcctccctg gcagtttcat ccggaacccg gacgaaagta aaaatgcata
    18301 tgagttgcac taaaaaagcg actcacattg ttccgttata atgcctgaag tggatcacag
    18361 aatatatctt caggaatcgc atatctatta agttactcac tcttttgtat ttatgacatg
    18421 cgcgtgtttg tataaatgta aatgtgagtc cttgttccac tctcgtgcag catcgctggt
    18481 catacgcgaa cacgtacaaa cagcaatggt gaggcggcat caagagcagg gatccgtcat
    18541 ttatgcgagc attttacccc aaaaaccttt tatttataag ggtcattgcg aattatctga
//
LOCUS       DACBVI010000088        18418 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.088, whole genome shotgun sequence.
ACCESSION   DACBVI010000088 DACBVI010000000
VERSION     DACBVI010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.088"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..1168
                     /gene="tnpB"
                     /locus_tag="IU325_14625"
     CDS             <1..1168
                     /gene="tnpB"
                     /locus_tag="IU325_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP5810241.1"
                     /translation="LRATKVRIYPTPEQAEFINAQFGAVRFAYNKALHIKKHSYQRHR
                     VNLNPRKDLKPLLAVAKKSRRYGWLKEYDSIALQQAVINLDVAFSNFFNPKLKAGKPT
                     FKSKHGRQSSYHCVGIKVLDAAIKIPKILPIEARLHREITGMLKSITLSRSATGKYYA
                     SLLCDDGMEAPAKPALISNVTGLDMGLAHFVIKSDGNKIASPRHLINASRNLRRKQKA
                     LSRKKKGSANRRKARIQLAAVHERVAGARADFQHKLSRAIVDENQAVIVETLKMANMM
                     KNHHLARAIGDAGWSGFITKLEYKATEKGVHLVKLDQWFASSKTCHCCGHKMSEMPLH
                     KRIWQCPECGVEHDRDINAALNIKRKGILELQAAGLVVSAHGGQRKSVTQTVAA"
     gene            complement(1332..1991)
                     /locus_tag="IU325_14630"
     CDS             complement(1332..1991)
                     /locus_tag="IU325_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HAP5810242.1"
                     /translation="MANWLNQLQSLLGQSSSSASSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(2318..2674)
                     /gene="yebF"
                     /locus_tag="IU325_14635"
     CDS             complement(2318..2674)
                     /gene="yebF"
                     /locus_tag="IU325_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HAP5810243.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(2741..3031)
                     /gene="yebG"
                     /locus_tag="IU325_14640"
     CDS             complement(2741..3031)
                     /gene="yebG"
                     /locus_tag="IU325_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HAP5810244.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            3165..4343
                     /gene="purT"
                     /locus_tag="IU325_14645"
     CDS             3165..4343
                     /gene="purT"
                     /locus_tag="IU325_14645"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HAP5810245.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADNENLFRKAVAEIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAHAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSQLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSEN
                     VTFDNVQNAVGAGLQIRLFGKPEIDGSRRLGVALATAESVDEAIERAKHAAAQIKVQA
                     "
     gene            complement(4398..5039)
                     /gene="eda"
                     /locus_tag="IU325_14650"
     CDS             complement(4398..5039)
                     /gene="eda"
                     /locus_tag="IU325_14650"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP5810246.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(5076..6887)
                     /gene="edd"
                     /locus_tag="IU325_14655"
     CDS             complement(5076..6887)
                     /gene="edd"
                     /locus_tag="IU325_14655"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HAP5810247.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(7122..8597)
                     /gene="zwf"
                     /locus_tag="IU325_14660"
     CDS             complement(7122..8597)
                     /gene="zwf"
                     /locus_tag="IU325_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP5810248.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            8935..9804
                     /gene="hexR"
                     /locus_tag="IU325_14665"
     CDS             8935..9804
                     /gene="hexR"
                     /locus_tag="IU325_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator HexR"
                     /protein_id="HAP5810249.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            9932..11374
                     /locus_tag="IU325_14670"
     CDS             9932..11374
                     /locus_tag="IU325_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase II"
                     /protein_id="HAP5810250.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(11506..12477)
                     /gene="lpxM"
                     /locus_tag="IU325_14675"
                     /gene_synonym="msbB"
     CDS             complement(11506..12477)
                     /gene="lpxM"
                     /locus_tag="IU325_14675"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAP5810251.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(12597..13919)
                     /gene="mepM"
                     /locus_tag="IU325_14680"
     CDS             complement(12597..13919)
                     /gene="mepM"
                     /locus_tag="IU325_14680"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HAP5810252.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(13935..14867)
                     /gene="znuA"
                     /locus_tag="IU325_14685"
     CDS             complement(13935..14867)
                     /gene="znuA"
                     /locus_tag="IU325_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HAP5810253.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLENVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTEKQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLNQLANQYASCLKGD"
     gene            14946..15701
                     /gene="znuC"
                     /locus_tag="IU325_14690"
     CDS             14946..15701
                     /gene="znuC"
                     /locus_tag="IU325_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HAP5810254.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            15698..16483
                     /gene="znuB"
                     /locus_tag="IU325_14695"
     CDS             15698..16483
                     /gene="znuB"
                     /locus_tag="IU325_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HAP5810255.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(16633..17643)
                     /gene="ruvB"
                     /locus_tag="IU325_14700"
     CDS             complement(16633..17643)
                     /gene="ruvB"
                     /locus_tag="IU325_14700"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP5810256.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATVRAWNHFGITPPE
                     MP"
     gene            complement(17652..18263)
                     /gene="ruvA"
                     /locus_tag="IU325_14705"
     CDS             complement(17652..18263)
                     /gene="ruvA"
                     /locus_tag="IU325_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP5810257.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT         4450 a         4771 c         4601 g         4596 t
ORIGIN      
        1 gttaagagca acaaaagtac gcatctaccc gacaccagaa caggctgaat ttatcaacgc
       61 ccagttcggt gcagtgcgtt ttgcgtacaa caaagctctg catatcaaga aacattctta
      121 ccagcggcac cgcgtaaatt taaacccgcg taaagacctc aaaccactgc ttgcagtggc
      181 gaagaaatcc cgccggtatg gatggcttaa agaatatgat tctattgcgt tgcagcaggc
      241 ggtaattaac ctcgacgtgg ctttttctaa ctttttcaat ccgaaactga aagcaggtaa
      301 acctaccttt aaaagtaaac acggcagaca atcgagctac cattgtgttg gcataaaagt
      361 ccttgatgct gcgataaaaa taccgaaaat cttgccgatt gaagcgcgtt tacatcgtga
      421 aattacggga atgctgaaaa gtatcacgtt gagccgcagc gcaaccggga aatactacgc
      481 gtcgttactc tgtgacgacg ggatggaagc gccggcaaag cccgcactga tatcaaatgt
      541 gacagggctg gatatggggc tggcgcattt tgtcatcaaa tcggacggca ataaaattgc
      601 cagtccccgc cacctaatca atgcaagtcg caacctgcgc cggaagcaga aagcactgtc
      661 ccgtaagaaa aaagggagcg caaacaggcg caaagccaga atacaacttg cagccgttca
      721 cgaacgggta gccggtgccc gtgccgattt ccagcacaag ttatctcgcg caattgttga
      781 cgaaaaccaa gcggtaattg tcgagacatt gaaaatggca aatatgatga aaaatcacca
      841 tctggctcgt gcgataggcg atgctggatg gtctggattc atcacaaaac tggaatacaa
      901 agcaacagag aaaggtgtgc atctggtgaa actggatcaa tggttcgcca gttcgaagac
      961 ctgccactgc tgcggtcaca aaatgtcgga gatgccatta cataagcgca tctggcagtg
     1021 tccagagtgt ggagttgaac atgaccgcga tatcaacgcg gcgctcaaca tcaaacgcaa
     1081 gggcatactg gaattacaag cggcgggact cgtcgtttct gcccatggag gccagcgtaa
     1141 atccgtcaca cagacggttg cggcttaaga agtgggaagc ctcgcccgac agggcgggga
     1201 gcagtcacat gatgacaggt aatggcgcgg atatcgaatg ttatgcagac agagaaaatc
     1261 agcctgctca aatggctgtg cggttctgga tagcccgaaa tagtcaactt caggctatcc
     1321 agagagcgga attattccgc caaagtgcgt ttttgttgtt cgagatcgcg ttcaatgcct
     1381 tcacgaacgt cctgggggat tttcagcgcg tcacccagtg cattcaggta actgcgttcc
     1441 ataaaatggt caatatcaat agccgcgcag ctcaggaaat agatttccag cgcctcttct
     1501 tcattgcgga ccccggtagc caggcgttgt ggatccagcg gttgttcgat tgcctgctca
     1561 atgagtacac gcccctgctc ttccacgccg gcttcacgca attgctgatc gattgccgca
     1621 cgttctttgg catcaatatg accatcactt ttagcggcaa agaccagcgc aaggatcaaa
     1681 cgttcagtac gctcatccag cggcgtactt tgcgcgccaa actgcggttc gtcctgatgc
     1741 gccgcgcgaa ttttatcttt gtatttattc cacagcaccg tacccgctac cgctccgccg
     1801 ccaaccagca acgcgttggt gccatatttc gtaagaagtt tacgtgctga tttatttgcg
     1861 accagcagcc cagccagtcc gcctaatgcg cccggcacta aaagtttgcc caatccctga
     1921 tccgcagacg aggaggcaga agaactgctt tgcccaagca gggattgcag ttgatttaac
     1981 cagttagcca tatttgctcc tcaataacca tttttagtgc ctgtagcata gcggatgagg
     2041 atgtcagaaa atgtatatcg agacaaaaga tgcgcaaata agcggggcgg ttggccgtta
     2101 ttcaaagaaa ttcccgccag gtaaggtgtt cactgtatat cagatgataa gggtatttta
     2161 ggtgaaagta gtttgacgtt ttgagtagca ctgtttgatg ctgattgccc gatgcgacgc
     2221 tgttgcgtct tctcaggcct acgattgtgg tgcgttgtgg ggcggataag gcactcgcgc
     2281 cgcatccgcc aacggtcaat cgaattatca gaagccatta acgccgctga tattccgcca
     2341 ttcccgcttt gcagtccacg ctgacctgat aatgaatatc ggcactttta ccacgcacgg
     2401 ttagcggtac tgaccattta tcatctttac cctgaatgtc ctgcaaactg acccaagcca
     2461 cgggatcggc ctgaccgaca attttttgat catctgccca gcgcgccacg cgattttgtt
     2521 gataatcacg ttttacgctc gcggcaattc cggcagcatc cagaccttca cactttggga
     2581 aagtgaccga cttgctggtt tcattattgg cagcgaaaac tgatgcgcag gcagaaacca
     2641 acaacagccc taaaaacgcc cctctttttt tcatgttttt ctccatagca caatgattca
     2701 ggagaaagca tggtacaaat tgtcaggagc gcaagttgct tcaggccgcg tgtgaggcat
     2761 cttcctcttc ggattcagca ccgacgacct gtggggacgg taatttgccg gttttcagaa
     2821 tggtgctcag cacatctttt tgttctgcca gccatagcga aagggcttca cgttgctcgt
     2881 cttccatttg cactggagaa tttgtcagcc aggtgtcaag cagatccgcc gtatcaagca
     2941 ttttgtcata agcatcggct tcctttttgc tggtaaacga cattttctct tcgccctcac
     3001 gaatgactac gtatttaact tcaaccgcca tttgcagcct ctcataataa ctgtgatttt
     3061 atacagtata tttcttttcg gttggaaaat caatatcagc aataaagaca cgcgcaaacg
     3121 ttttcgttta tactgcgcgc ggaattaatc aggggatatt cgttatgacg ttattaggca
     3181 ctgcgctgcg tccggcagca actcgcgtga tgttattagg ctccggtgaa ctgggtaaag
     3241 aagtggcaat cgagtgtcag cgtctcggcg tagaggtgat tgccgtcgat cgctatgccg
     3301 acgcgccagc tatgcatgtc gcgcatcgct cccatgtcat taatatgctt gatggtgatg
     3361 cattacgccg tgtggttgaa ctggaaaaac cacattatat cgtgccggag atcgaagcta
     3421 ttgccaccga tatgctgatc caacttgaag aggaaggact gaatgttgtc ccctgcgcgc
     3481 gcgcaacgaa attaacgatg aatcgcgagg gtatccgtcg cctggcggca gaagagctgc
     3541 agctgcccac ttccacttat cgttttgccg ataacgaaaa ccttttccgc aaggcggttg
     3601 ctgaaatcgg ctatccctgc attgttaaac cggtgatgag ttcctccggc aaggggcaga
     3661 cgtttattcg ttctgcagag caacttgctc atgcatggga gtacgctcag caaggcggtc
     3721 gcgccggagc gggccgcgta attgttgaag gcgtcgttaa gtttgatttc gaaattaccc
     3781 tgctaaccgt cagcgcggtg gatggcgtcc atttctgtgc accggtaggt catcgccagg
     3841 aagatggcga ctatcgtgaa tcctggcaac cacagcaaat gagccagctg gctttggaac
     3901 gtgcgcaaga aattgcccgt aaagtggtgc tggcactggg cggttatggg ctgtttggtg
     3961 tcgagctgtt tgtctgtggt gatgaggtga ttttcagtga ggtctcccct cgcccacatg
     4021 ataccgggat ggtgacgtta atttctcagg atctctcgga gtttgccctg catgtgcgag
     4081 cctttctcgg acttccggtt ggcgggatcc gtcagtatgg tcctgcagct tctgccgtta
     4141 tcctgccaca actgaccagt gagaatgtca cgtttgataa tgtgcagaat gccgtaggcg
     4201 caggcctaca gattcgttta tttggtaagc cggaaattga tggcagccgt cgtctgggtg
     4261 ttgcgctggc gaccgctgag agtgttgatg aagcgattga gcgcgcaaag cacgccgcag
     4321 cacagataaa agtacaagct taaccccggc gaaaaacgct accaaaatgc ccgatcgagg
     4381 atcgggcatt ttgactttta cagcttagcg ccttctacag cttcacgcgc cagcttagta
     4441 atgcggtcgt aatcgcccgc ttccagcgca tctgccggaa ccagccagga accaccgatg
     4501 cacagcacgc ttttcagcgc caggtagtca cggtagttag ccggagaaat accacccgtc
     4561 gggcagaaac ggacctggga gaacggaccc gcgatcgcct gcagggcttt cacaccgccg
     4621 ttagcttcag ccgggaagaa tttgaactct ttcaaaccgt agtccatacc cagcatcagt
     4681 tcggaaacag tgctgatccc cggaatcaga ggaatagtcc cttcggtagc agccttcagc
     4741 agcggctcgg tcagacccgg gctaattgcg aactgtgcac ccgcttcggt gacttctgcc
     4801 agctgctgtg gattcagcac cgtaccggca cccacaatcg cttcaggcac ttctttggcg
     4861 atagcacgga tagcgtcaac tgcacactcg gtacgcagag tcacttccag aacgcgcacc
     4921 ccaccagcaa ccaacgcttt tgccatcggc accgcgtgtt ccagtttttt taccacgata
     4981 accggtacaa ccgggccggt ggtcaggatt gattctgcac ttgttttcca gtttttcatc
     5041 agagttttct ctcgcctgat tacaaatttg tcgtcttaaa aagtgataca ggttgcgccc
     5101 tgttcggcac cggacagttt ttcacgcaag gcgctgaata attcacgtcc tgttcccacg
     5161 cgtgacgcgc tcaggtcagg aatgtgtggt tcgcgagcag ccagttccgc ttcgtctacc
     5221 agcagcgtca gttcgcctgt ctgtccattc acacgaatga tgtccccgtc gcgcactttt
     5281 gccagcagcc cgccatcgta ggcttctggt gttacgtgga tagctgacgg cactttacct
     5341 gaagcgccgg agagtcgtcc atcagtaact aacgcaattt tgaaacaccg gtccaataat
     5401 acaccaagtg gcggcatgag tttatgtaat tctggcattc cgttcgcttt tggcccctga
     5461 tgacggacaa caacgacaca atcgcggtcc agcaaacccg cttcaaaagc cggcataacg
     5521 tcatgctggc tttcaaaaac aaccgcaggc gcttcaatca cctggttctc aaccggcacg
     5581 gcagaggttt tcataaccgc acggcccagg ttaccgctta acacttttgt cccaccatga
     5641 tgagagaaag gttgttcgaa ggaagcgatc acattgctgt cgagtgattt ttccgcccct
     5701 tcccgccagt ccagttcacc attattcagc catggttcaa gggtataacg agacagacca
     5761 aaaccagcca ccgtattgac atcttcatgc agcaggcctg ctttgagcag ttcacgcacc
     5821 agaaccggta cgccacctgc cgcctggaag tggttaatat cggccggacc gttcgggtag
     5881 agacgtgcca tcagcggtac aacatcagaa aggtcagaga agtcatccca gttaatctga
     5941 ataccggccg cgcgagccat cgccaccagg tgcatggtgt ggttagtgga accaccggtc
     6001 gccagcagtg caacgatacc gttcaccacc actttctcat cgatcatctt accgatcggc
     6061 atccattcat taccattacc ggtcatgcgt gtaacctggc gcgcagcggc tgcggtcaaa
     6121 gcatcgcgca gcggagaatc cggatgaaca aaagaagagc ctggcaactg catccccata
     6181 aactccacca ccatctggtt ggtgttggca gtaccgtaga aagtacatgt tcccggcgca
     6241 tgataagacg cggcttctga ctccagtaat gccatgcggt ccactttacc ttcggcataa
     6301 agctggcgaa tacgcacttt ttctttattt ggcaaaccgc ttgccatcgg tccagacggc
     6361 acaaacaccg caggcaaatg cccaaacgac agggctgcca tagtcagacc cggaacaatc
     6421 ttgtcgcaca ctccgaggaa cagcgcacca tcaaacatgt tatgggacaa ccccaccgcc
     6481 gcagacatcg caatcacttc gcggcttaac agcgacaatt ccattccatc ctgcccctgg
     6541 gtgacaccat cacacatcgc cggaacaccg cccgcaacct gaccaaccgc atttgcttca
     6601 tgcagggctt tacgaatgat ttctggatag tgttcataag gctggtgcgc ggagagcatg
     6661 tcgttatagg aggtgatgat ggcgatattg ttacgcaaca tgcttttcaa agaggctttg
     6721 tcttctggct ggcaggcagc gaaaccgtgt gccaggttac cacatgccaa ctgcgaacga
     6781 tgaacggtcg aagttttcgc ttgttctatc cgggcgaggt aagcagagcg agtctcgcgc
     6841 gaacgttcaa tgattcgatt tgttacgcgt aacaattgtg gattcataaa ggctcctgaa
     6901 attgagttgt cagagcagga tgattcacaa cgcgcttcat tcagaggatt tatgactgaa
     6961 acgcctgtaa ccggagctca tagggcaaaa acgtttcagt cagtgtaata aaaaaagcct
     7021 cgtgggtgaa tccgcacgag gcctgaaagt gtaaaaattg ttctacaatc tgtgcaagat
     7081 catgttaccg gtaaaataac cataaaggat aagcgcagat attactcaaa ctcattccag
     7141 gaacgaccat cacgggtaat cattgccacc gaggcaacgg gtccccaggt tccggcctga
     7201 tacggtttcg gcgcatcatt gtccatcgcc cacgcctcag taatggagtc tacccatttc
     7261 caggcttctt ccacttcatc acgacgaaca aacagtgcct gaataccacg catggtttcc
     7321 agcagcagac gttcataggc atccgccaga tgcgtctgat taaaggtttc tgaatagctc
     7381 aggtccagct tggtgatttg caggttatgt ttgtggtcaa ggccaggaac tttattcagt
     7441 acctggatat ccacgccttc atcaggttgc agacggatag tcagtttatt ttgcggtaga
     7501 tcctgccacg actctttaaa cagattcagt tcaggtgttt tgaaatagac cacgacttca
     7561 gagcatttgg tcggcagacg tttaccagta cgcaggtaga atggcacacc ggcccagcgc
     7621 cagttatcaa tgtcgacgcg gatcgccacg aaggtttctg tattgctgct cttgttcgcg
     7681 ccctcttctt ccagatatcc cggcactttt ttgccctggg cgaagcccgc agtatattgc
     7741 ccgcgtacgg ttttttcgcg tacgttggag cggtcaatac ggcgcagaga cttcagtact
     7801 ttcacttttt catcgcggat gctgtctgca ctcaggtcag acggcggaga catcgcaatc
     7861 atgcaaagaa tttgcagcag gtggttctgg atcatgtcgc gcatctgacc ggctttatca
     7921 aaatagcccc agcgcccttc gatccccact tcttctgcca cggtaatctc aacatgatcg
     7981 atggtgcgat tgtcccagtt gttcacaaac agagagttag caaaacgcag cgccaacagg
     8041 ttcagcaccg tttctttacc aagatagtgg tcgatacggt aaacctggca ctcctcgaag
     8101 tattcgccaa cctgatcgtt gatttcctgc gaggtcgcca gcgacgtccc cagcggtttc
     8161 tccatgacta cgcgtgccgg tttagcattc agttttgcct cgccaagccc tttgcaaatt
     8221 gcgccaaaag tgctgggcgg catggcaaag tagttaatgg tgatacgatt tttttgatcc
     8281 agcatcgcgc cgagacggct gaatgcagca gtgtcattga catcgagatt acaaaaatcc
     8341 agacgtgcac tcagggtgtc ccataaacct tcatcaatgg tttctttcat gaaagtttcg
     8401 agcgcctcgc ggacaacttt ggtataagcc gctttatccc agtcagcacg ccctacgccg
     8461 ataatccggg tgtccgggtt gagctgaccg gctttttcca gttgatacag ggaaggcagc
     8521 aatttacgac gcgcaaggtc gcctttcgcg ccgaaaatga ccaggtcaca ggcctgggct
     8581 gtttgcgtta ccgccatgtc attctcctta agttaactat cccggtactt aagccagggt
     8641 atacttgtaa ttttcttacg gtgcactgta ctgcttttac gagcttgcga aaactgtaaa
     8701 cgcttatcca cccgtgcgat tacggtaaaa gcgcgcaaag tgcggcaaaa ctgataaaaa
     8761 aatcatcgtt ttccattggg taaaaatctg acactgatca tgttatgaaa aaaaataaca
     8821 acttttttat ctgcttttgt cattaacaca gcacacaggc gtaatatctg acaaaactgc
     8881 atttcgattt ctttcagtgc ggaaatcgtc attacccgtg agtctcttta catcatgaat
     8941 atgctggaaa aaatccagtc tcagctggaa catttgagca aatcagagcg caaagttgcc
     9001 gaggtcattc tggcttcgcc cgataacgcg atccattcga gtattgctgc tctggcgctg
     9061 gaagccaatg ttagcgaacc gacggtgaat cgtttctgtc gcagcatgga cacgcgcggt
     9121 tttcctgatt ttaaacttca tctggcacaa agtctggcga atggcactcc ctatgttaat
     9181 cgcaacgtca atgaagatga cagcgttgaa tcatacacgg ggaaaatatt tgagtccgca
     9241 atggcaacgc tcgatcatgt ccgtcattca ttggataaat ctgccatcaa ccgcgccgtc
     9301 gacttactca ctcaggcaaa aaaaatcgcc tttttcggat taggctcttc tgccgctgtt
     9361 gcccacgatg cgatgaataa gttctttcgt tttaatgttc cggtggtgta ctccgatgat
     9421 attgtgctgc aacgcatgag ttgtatgaat tgtagcgacg gagacgtggt ggtgctgatt
     9481 tctcacacgg gaagaacaaa aaatctggtc gagctggcgc agctggcacg cgaaaacgac
     9541 gccatggtga ttgccctcac ctctgcaggt accccgctcg cccgggaagc cacgctagca
     9601 attaccctcg acgtaccgga agatactgac atttatatgc ccatggtttc tcgacttgca
     9661 cagctgaccg tgatagatgt gctggcgaca ggatttactt tgcgacgcgg tgcaaaattc
     9721 agagataact tgaagcgggt caaggaagcg ctgaaggaat cgcgttttga taagcagtta
     9781 cttaatttaa gtgacgatcg ctaaaaacga ctgtcactgt cctaatctta tacgacatcc
     9841 gaatgagatt aatttatagc catcgcggcg ttatttcatt cggatttcat gttcaagcaa
     9901 cacctggttg tttcagtcaa cggagtatta catgtccaga aggcttcgca gaacaaaaat
     9961 cgttaccacg ttaggcccag caacagatcg cgataataat cttgaaaaag ttatcgcagc
    10021 gggtgccaac gttgtacgta tgaacttttc tcacggctcg cctgaagatc acaaaatgcg
    10081 cgcggataaa gttcgtgaaa ttgccgcaaa actggggcgt catgtggcta ttctgggtga
    10141 cctccagggg cccaaaattc gtgtatccac ctttaaagaa ggcaaagttt tcctcaatat
    10201 tggggataaa ttcctgctcg acgccaacct gggtaaaggt gaaggcgaca aagaaaaagt
    10261 cggtatcgac tacaaaggcc tgcctgctga cgtcgtgcct ggtgacatcc tgctgctgga
    10321 cgatggtcgc gtccagttaa aagtactgga agttcagggc atgaaagtgt tcaccgaagt
    10381 caccgtcggt ggtcccctct ccaacaataa aggtatcaac aaacttggcg gcggtttgtc
    10441 ggctgaagcg ctgaccgaaa aagacaaagc agacattaag actgcggcgt taattggcgt
    10501 agattacctg gctgtctcct tcccacgctg cggcgaagat ctgaactatg cccgtcgcct
    10561 ggcacgcgat gcaggctgtg atgcgaaaat tgttgccaag gttgaacgtg cggaagccgt
    10621 ttgcagccag gatgcaatgg atgacatcat cctcgcctct gacgtggtaa tggttgcacg
    10681 tggcgacctc ggtgtggaaa ttggcgaccc ggaactggtc ggcattcaga aagcgttgat
    10741 ccgtcgtgcg cgtcagctta accgcgcagt aatcacggcg acccagatga tggagtcaat
    10801 gattactaac ccgatgccga cgcgtgcaga agtcatggac gtagcaaacg ccgttctgga
    10861 tggtactgac gctgtgatgc tttctgcaga aactgctgct ggtcagtatc cttcagaaac
    10921 tgttgcagcc atggcgcgcg tttgcctggg tgcggaaaaa atcccgagca tcaacgtttc
    10981 taaacaccgt ctggacgtgc agttcgacaa tgtggaagaa gctattgcca tgtcagcaat
    11041 gtacgcggct aaccacctga aaggcgttac ggcgatcatc accatgaccg aatcgggtcg
    11101 tacggcgctg atgacctccc gtatcagctc tggtctgcca attttcgcca tgtcacgcca
    11161 tgaacgtacg ctgaacctga ctgctctcta tcgcggcgtt acgccggtgc actttgatag
    11221 cgctaatgac ggcgtagcag ctgccagcga agcggttaat ctgctgcgcg ataaaggtta
    11281 cttgatgtct ggtgacctgg tgattgtcac ccagggcgac gtgatgagta ccgtgggttc
    11341 tactaatacc acgcgtattt taacggtaga gtaagtacgt tgccggatgc ggcgaaaacg
    11401 cctcatccgg cctacagttc aattatagtt caacagattt cgaatcttct gaagcaaact
    11461 tgaacttatc acaaggcgga ggcctctcct cgcgagaggc tttttttatt tgatgggata
    11521 aagatctttg cgcttatacg gttggatttc gcccggcttg cgagttttca gcaattttaa
    11581 tatccaggtg tattgttctg gtcgcggacc aacaaaaatc tcgacttctt cattcatccg
    11641 ccgcgcaatc gtatggtcat ctgcctctaa cagatcatcc atcggtgggc gcacctgaat
    11701 cgtcaggcga tgcgtcttgc catcataaat cggaaacagc ggtacaacgc gcgcacggca
    11761 cactttcatc aaacgaccaa tcgcgggcaa cgtcgcttta taggtggcaa agaaatcaac
    11821 aaattcgctg tgctctgggc catgatcctg atcgggtaaa taatatcccc agtacccctg
    11881 acgtaccgac tggatgaatg gtttaatacc atcatttctc gcatgcagac gaccgccaaa
    11941 acgacgacgc accgtgttcc agacataatc aaaaaccggg ttgccctgat tatggaacat
    12001 cgctgccatt ttctgccctt gcgaggccat cagcatggca ggaatatcga cggcccaacc
    12061 gtgcggcacc agaaagatca ctttctcgtt attacgtcgc atctcttcga taatctccag
    12121 cccttgccag tcaacgcgcg gctgaatttt ctccggcccg cgtattgcca actcagccat
    12181 cattgccatc gcttgcggcg cggtggcaaa catctcatca acaatcgctt cgcgttcagc
    12241 ttcactacgt tctggaaagc agagcgacaa attgattaac gcacgacggc gtgagctttt
    12301 tcccaggcgt ccggcaaaac gtcccagccg tgccagaatg gggtcgcgaa actttggcgg
    12361 cgttaaagca atacccgcca tcgctgctac gcccagccat gctccccagt agcgcgggtg
    12421 gcgaaaggat ttatcaaact caggaatgta ttcgctatta ttttttttcg tttccatgct
    12481 tttccagttt cggataaggc aaaaatcaat ctggtgatag tgtagcggcg caacttgccc
    12541 cgcaccaaat aaaaaaagcc ggtactgact gcgtaccggc tgcgaatgga tgttaattaa
    12601 tcaaaccgta gctgcggcac aatctctttg gcctgcgcca ggaattcgcg acgatcggag
    12661 ccggtcagcc cttcggtacg cggcagtttt gccgtcagcg ggtttacggc ctgctggttt
    12721 atccatactt catagtgcag atgcggcccg gttgaacgtc cggtattacc ggaaagcgcg
    12781 atacggtcgc cacgtttcac cttctgtccc ggtttcacca gaatcttgcg caagtgcata
    12841 taacgcgtgg tgtagctgcg accatgacga atagccacat aataacctgc tgcgccactg
    12901 cgtttggcaa ccaccacttc accgtcaccc actgaaagca ccggcgtacc ctgtggcatg
    12961 gcgaaatcaa cacctctgtg tggcgcaaca cgaccagtca ccggattagt acgacgcggg
    13021 ttaaagttag acgagatacg gaactgtttc gccgtcggga atcgcaagaa tcctttcgcc
    13081 agaccagtac cgttacgatc atagaatttg ccatcttcag cgcggattgc gtaataatct
    13141 ttaccttctg aacgcaaacg tacgcccagc agttggcttt gctcacgttt accatcaagc
    13201 atttctcgtg acattaacac cgcaaattca tcgccttttt tcagtttgcg gaaatccatt
    13261 tgccactgca tggctttaat cactgcgctc acttcggcgc tggttaaacc ggcgtttctg
    13321 gcgctggcaa caaagcttcc cccgacggta cctttcagca gattgttgac ccactctcct
    13381 tgctgcattt cgctggtcat tttaaaaccg ttagcggcag tacggtcata ggttcgggtt
    13441 tcacgacgag acacttccca ggtgaggcgc tgcagttcgc cgtccgcggt taatgtccag
    13501 gagagttgtt gaccgatttt caggttacgc aattctttgt cggcagcagc cagttgggtg
    13561 atatcaccca tatcaatacc atactgattg agaatgctgc ttagcgtatc gccagtggaa
    13621 acaacatatt catgcacgcc cgcttcaccg gcgattttgt catccagttc atcctgggga
    13681 atggcttcat cttcttgtgc agcttgatca atcggctcac tggcttcagg taagagcgaa
    13741 cgaatttcgt tctgttccag ctcaatggtt ttgacaattg gcgtggcatc gcggtgataa
    13801 acatagggcc gccagacagc gacggccaga gtaagaacgg tgagcgaccc caacataacg
    13861 cggtgtggtc gcggtaaatt attaaacgcc agggcgacag agcgggctat ctgttgcacg
    13921 taatcacttc ctcattaatc tcctttcagg cagctcgcat actggttggc taattgattc
    13981 aggaattctg aatagcttgt tttacccagt ttgatattcg tccccagggg atccaacgtt
    14041 cccatacgaa cggatgtccc tcgtgcgacg ctctcaacga ccgctggcct gaactgtggc
    14101 tcagcaaaaa cgcaggttgc tttttgctca accaactgtg ttcttatttc atgtaaacgc
    14161 tgcgcgccag gttgaatctc agggttaacg gtaaaatgac caagcggtgt cagtccgaac
    14221 tgtttttcga aatagccgta agcatcgtga aaaacgaaat aacctttccc cttgagcggc
    14281 gcgagctcgt taccaacctg cttttcggtt gaggctaatt gtgcctcaaa atccttcagg
    14341 ttggcgtcaa gtttggctcg actttgcggc ataagttcca ctaattttcc atggattgca
    14401 accgctgtag cccgcgctat ctctggggaa agccaaagat gcatgttgaa atcgccgtga
    14461 tggtgatctt cgtcactttt ttccgcgtgg tcgtgatcat catcatcgcc gtgaatactt
    14521 ttcatcagca gcggtttcac attctctagc tgcgcaatcg ttacctgttt cgcttcaggt
    14581 aatttactta ccggtttttg catgaacgct tccatctccg ggccaaccca aacgactaag
    14641 tccgcgttct gtaagcgttt tacatctgat ggacgcagtg aataatcatg ttctgaagcg
    14701 ccgtcaggta gtaaaacctc cgtttctgtt accccatcag caatggcaga agcgatgaac
    14761 ccaacgggtt taagcgaagc gacaacggca gcatctgcgg cctgtgttgc accgccccag
    14821 agagcggcgg ataatgctgc gaaaagaagc gtttttttat gtaacataat gcgaccaatc
    14881 atcgtaatga atatgagaag tgtgatatta taacatttca tgactactgc aagactaaaa
    14941 ttaacatgac aagtctggtt tccctggaaa atgtctcggt ttcttttggc caacgccgcg
    15001 tcctctctga tgtgtcgctg gaacttaaac ctggaaaaat tttgacttta cttgggccaa
    15061 atggcgcagg taagtcgaca ctggtacggg tagtgctcgg gctggtaaca cccgatgaag
    15121 gggttatcaa gcgcaacgga aaactgcgca tcggctatgt accgcagaag ctgtatctcg
    15181 acaccacgtt gccacttacc gtaaaccgtt ttttacgctt acgccctggc acacataaag
    15241 aagatatttt gcctgcactg aaacgtgtcc aggccgggca tctgattaac gcaccgatgc
    15301 aaaagctctc gggtggcgaa acacagcgtg tattgttagc gcgagcattg ttaaatcgac
    15361 cgcaattatt agtgctggat gaacccactc agggcgtgga tgtgaatggt caggtggcgt
    15421 tatatgacct tattgaccag ttgcgtcgcg aactggattg tggcgtttta atggtctctc
    15481 acgatctgca tctggtaatg gcaaaaaccg atgaagtgct ttgcctgaat caccacattt
    15541 gttgttccgg tacaccggaa gttgtttccc tgcatccgga gtttatttct atgtttggtc
    15601 ctcgtggtgc tgaacaactg ggtatctatc gccatcatca taatcatcgt cacgatttac
    15661 agggacgaat tgttttgcgt cggggaaatg atcgctcatg attgaattat tatttcccgg
    15721 ttggttagcc gggatcatgc tcgcctgtgc cgcgggtccg ctgggttcgt ttgtggtctg
    15781 gcgtcgtatg tcttatttcg gtgatacgct ggcccatgcc tcattacttg gtgtcgcgtt
    15841 tggtttgttg ctggacgtga atccattcta tgcggtgatt gcagttacgc tgctgctggc
    15901 gggcggtctg gtatggctgg agaagcgtcc acagctggcg atcgacacgt tattagggat
    15961 aatggcgcac agtgccctgt cgctgggcct ggtggtcgtt agtctgatgt ctaatattcg
    16021 tgttgatttg atggcttacc tgttcggtga tttgctggca gtgacgccag aagatctcat
    16081 ctctattgca attggcgtgg tcatcgtggt ggcgattttg ttctggcaat ggcgcaattt
    16141 gctgtcgatg acgattagcc cggatctggc gtttgttgat ggtgtgaaat tacagcgcgt
    16201 gaaattgttg ttgatgctgg tgacggcatt gacgattggt gtagcgatga aatttgtcgg
    16261 cgcgttgatt attacttcgt tgctaattat tcctgctgct actgcacgtc gctttgcccg
    16321 cacgccggaa cagatggcag gtgtcgctgt tttggtgggg atggtggcag tgactggcgg
    16381 tttaaccttt tccgcatttt acgatacacc agcaggcccg tcggtggtgc tatgcgcggc
    16441 actgttattt attctcagta tgatgaaaaa acaggccagc taatctttcg ctgaacacat
    16501 ttgtcggatg cggcgcgagc gccttatccg acctgtggtt cgctatctct ggtatatgcc
    16561 tgataagacg cgaatagcgg cgcatcaggc aacactgcca gtgtcggatg cgccgcgagc
    16621 gaccgatccg acttacggca tttctggcgg cgtgatgccg aagtggttcc acgcccgcac
    16681 tgtcgccata cgcccgcgcg gtgtacgctg caaaaagcct tgctgaatca aataaggttc
    16741 cagcacatcc tcaatggttt cacgttcttc gccaatggca gccgccaggt tatccagacc
    16801 taccggccca ccaaagaact tatcgattac cgccagcaac aatttgcggt ccatataatc
    16861 gaaaccttcg gcatcgacat tcagcatatc cagcgcctga gcagcgatat cagccgagat
    16921 ggtgccatcg tgcttcactt cggcgaagtc acgcactcga cgcagcagac ggttggcaat
    16981 acgtggcgta ccgcgagcgc gacgagcaac ttccagcgcg ccgtcatcac tcatctcaag
    17041 ccccataaag cgtgcgctgc gactgacgat atattgcaga tccggcacct gataaaactc
    17101 cagacgctgc acaataccaa aacgatcgcg caacggtgat gtcagcgaac ctgcgcgcgt
    17161 ggttgcacca atcagggtaa acggcggcaa atcaatttta atggagcgtg ccgccggacc
    17221 ttcaccaatc atgatatcca gttggtagtc ttccattgcc gggtacagca cttcttcaac
    17281 aactggcgat agacggtgga tctcatcaat aaacagcacg tcatgcggtt caaggttagt
    17341 gagcatcgct gccagatcgc cggccttttc cagcaccgga ccagaagtcg tgcgtaaatt
    17401 aacgcccatt tcattggcga caatgttggc aagcgtagtt ttacccaacc ccggaggacc
    17461 aaaaatcaac agatgatcga gggcatcgcc gcgcagtttc gctgctttga tgaaaatctc
    17521 catctgtgaa cgaacctgcg gctgaccaac atactcttcc agtaatttag ggcgaatggc
    17581 gcgatctgcc acatcttccg gcaaagtggt accggcagaa atcagacggt ctgcttcaat
    17641 catcctttac ctcataacgc ggcgcgtagg gcttcgcgaa ttaatgtttc actgctggcg
    17701 tcagggcgag cgattttgct caccatgcgg cttgcttctt gtggtttata gcccagcgcc
    17761 accagcgcgg caaccgcttc ttgttcagca tcgtcggtcg ccgggctggc aggagacgtg
    17821 agtaccaggt cggcggctgg cgtaaagaga tcgccatgca aacctttaaa tcggtctttc
    17881 atttcaacaa tcaagcgttc ggcggttttt ttgccaatac ccggcagttt caccagtgcc
    17941 cccacttctt cacgctcaac ggcattaacg aactgctgcg ctgacattcc ggagaggatc
    18001 gccagcgcta acttcgggcc gacgccgttg gttttgatca actctttgaa caatgtgcgc
    18061 tcttgtttat tgttaaaacc gtacagcagt tgcgcgtctt cacgcaccac aaagtgggtg
    18121 aaaacgatcg cttcctgacc cgcttcaggg agttcataaa aacaggtcat cggcatatgc
    18181 acttcatagc ctacgccgcc cacttcaatt aacaccagcg ggggttgttt ttcaatgatg
    18241 atgcctctga gtctgcctat cacatgacgc tcctgcgtaa tgaatcaaag ataatgctgt
    18301 atgataaaaa aatgctggat agatatccag cgaaggatga agaaaacttg cgaggtgtcc
    18361 cgatgatctg aaaaatggca cagtataatt tgttctacag gttatattgg aagcaaat
//
LOCUS       DACBVI010000089        18390 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.089, whole genome shotgun sequence.
ACCESSION   DACBVI010000089 DACBVI010000000
VERSION     DACBVI010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.089"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            39..464
                     /gene="nudI"
                     /locus_tag="IU325_14710"
     CDS             39..464
                     /gene="nudI"
                     /locus_tag="IU325_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HAP5810258.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLAHYDLNVATRKTLRLKGLL"
     gene            complement(503..1105)
                     /locus_tag="IU325_14715"
     CDS             complement(503..1105)
                     /locus_tag="IU325_14715"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase"
                     /protein_id="HAP5810259.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            1413..2552
                     /gene="arnB"
                     /locus_tag="IU325_14720"
     CDS             1413..2552
                     /gene="arnB"
                     /locus_tag="IU325_14720"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HAP5810260.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARLLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIALQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVNEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            2556..3524
                     /gene="arnC"
                     /locus_tag="IU325_14725"
     CDS             2556..3524
                     /gene="arnC"
                     /locus_tag="IU325_14725"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HAP5810261.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTKACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            3524..5506
                     /gene="arnA"
                     /locus_tag="IU325_14730"
     CDS             3524..5506
                     /gene="arnA"
                     /locus_tag="IU325_14730"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HAP5810262.1"
                     /translation="MKTVVFAYHDMGCLGIEALLSAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPEDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHSHAPAAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMHETIDETLDFFLRTVDLTDKPS"
     gene            5503..6393
                     /locus_tag="IU325_14735"
     CDS             5503..6393
                     /locus_tag="IU325_14735"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HAP5810263.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTLVDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGMPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGTLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            6393..8045
                     /gene="arnT"
                     /locus_tag="IU325_14740"
     CDS             6393..8045
                     /gene="arnT"
                     /locus_tag="IU325_14740"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HAP5810264.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLAAVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVASQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFAMDDAQHRAPFWYYLPVIIAGSLPWLGLLP
                     GALYAGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRNEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            8042..8377
                     /gene="arnE"
                     /locus_tag="IU325_14745"
     CDS             8042..8377
                     /gene="arnE"
                     /locus_tag="IU325_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HAP5810265.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            8377..8763
                     /gene="arnF"
                     /locus_tag="IU325_14750"
     CDS             8377..8763
                     /gene="arnF"
                     /locus_tag="IU325_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HAP5810266.1"
                     /translation="MGLMWGLFSVIIASAAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(8767..9024)
                     /gene="pmrD"
                     /locus_tag="IU325_14755"
                     /pseudo
     CDS             complement(8767..9024)
                     /gene="pmrD"
                     /locus_tag="IU325_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
     gene            complement(9134..10489)
                     /gene="menE"
                     /locus_tag="IU325_14760"
     CDS             complement(9134..10489)
                     /gene="menE"
                     /locus_tag="IU325_14760"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HAP5810267.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQDVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGEHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMAEGYWRNGQLVALVNDEGWYAT
                     RDRGELHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     D"
     gene            complement(10486..11448)
                     /gene="menC"
                     /locus_tag="IU325_14765"
     CDS             complement(10486..11448)
                     /gene="menC"
                     /locus_tag="IU325_14765"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HAP5810268.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDALPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPRKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLDKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVEVDALERLL"
     gene            complement(11448..12305)
                     /gene="menB"
                     /locus_tag="IU325_14770"
     CDS             complement(11448..12305)
                     /gene="menB"
                     /locus_tag="IU325_14770"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HAP5810269.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(12320..13078)
                     /gene="menH"
                     /locus_tag="IU325_14775"
     CDS             complement(12320..13078)
                     /gene="menH"
                     /locus_tag="IU325_14775"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HAP5810270.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEVFANYSRLY
                     VDLPGHGGSAAIRVDGFDDVTGLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVIVEGGHPGLQNAEQRTERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDEQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAAECHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(13075..14745)
                     /gene="menD"
                     /locus_tag="IU325_14780"
     CDS             complement(13075..14745)
                     /gene="menD"
                     /locus_tag="IU325_14780"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HAP5810271.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWHDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     EQHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKNERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTNGAQTLQQLLAQVSHL"
     gene            complement(14834..16129)
                     /gene="menF"
                     /locus_tag="IU325_14785"
     CDS             complement(14834..16129)
                     /gene="menF"
                     /locus_tag="IU325_14785"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HAP5810272.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQTFLRQHPKHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQRLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(16208..16513)
                     /gene="elaB"
                     /locus_tag="IU325_14790"
     CDS             complement(16208..16513)
                     /gene="elaB"
                     /locus_tag="IU325_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HAP5810273.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(16568..17029)
                     /locus_tag="IU325_14795"
     CDS             complement(16568..17029)
                     /locus_tag="IU325_14795"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP5810274.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMNKTLET
                     CTRHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            17094..18011
                     /gene="rbn"
                     /locus_tag="IU325_14800"
     CDS             17094..18011
                     /gene="rbn"
                     /locus_tag="IU325_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HAP5810275.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIVQPLTIYGPQGIREFVE
                     TTLRISGSWTDYPLEIVEIGAGEIFDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            18199..>18390
                     /locus_tag="IU325_14805"
     CDS             18199..>18390
                     /locus_tag="IU325_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HAP5810276.1"
                     /translation="MMVTVVSNYCQLSQTQLCQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKA"
BASE COUNT         4355 a         4898 c         4642 g         4495 t
ORIGIN      
        1 attctcatgg tttcttttta tttataagga atacccctgt gcgacaacgg actattgtat
       61 gccctttgat tcaaaatgat ggtgcttatt tgctgtgtaa aatggccgac gatcgcggcg
      121 ttttccccgg tcaatgggcg ctttcgggtg gcggcgtgga gcctggcgaa cgaattgaag
      181 aggcactacg ccgcgaaatt cgcgaagaac tgggagaaca gctgcttttg acagaaatca
      241 cgccgtggac cttcagcgat gatattcgca ccaagacgta tgcagatggt cgcaaggaag
      301 agatttatat gatttacctg atttttgact gcgtttctgc caaccgagaa gtgaaaataa
      361 acgaagagtt tcaggactac gcgtgggtaa aacctgaaga tctggcgcac tatgatttga
      421 atgtcgccac ccgaaaaacg ttacgtttga aaggtcttct gtaataatga tgacagccat
      481 tactggctgt cagattgaaa atttagtggt taacgaattc cccatccaga taaactttgc
      541 ctttttccac atgcatgact aaaccatcca gataatcagg tttaaatgtc gcatcacgct
      601 tatctttagc aatatatgtc aggcaatgat tatgggtgaa aataacgata tttttatcag
      661 gcgctttgct ttgtaagtcc ttaattgcac tataaatctc attaccgcac tgaagaagtc
      721 gtttatctac cgtcagcttt ttacccgctg aaaaccaggt cgccgactga atggtccgga
      781 cggtattact ggaataaaga tcgaaatcag ggatatcagc actaaaagcg ttgcccagtt
      841 cacgggcatc ctgggtacct ttgaccgtaa tacctgtttt atctgacaag cattgattgg
      901 ttgaacgatc gcaacgttca gcatggcgaa acaaaacgac aaccggatgc tgctgtgcca
      961 gtctggccag cgttttattg tcgatacgtg gcaaaccatt gctactccag gcggcatgag
     1021 tacccagtcc ggcaattgca gcgagcgcca gtaaaatgat gaaatatttt tttgacttca
     1081 acgaagagcg gcaaaaagct aacacgtaaa ctccacctat agacaagcgc aaccagacaa
     1141 ttaccgtgaa attgagcgac atttctggcg ataattagca gttggtgtaa tattaaaaat
     1201 cctgcgatgc cggcaaaatg cctcaaaatt ttgccaaatg caaagcctaa ataagaaaaa
     1261 atataaaaat ttcaatattt acgtctaata ttagtttctt aaggttaagt taatattcta
     1321 tccttaaaat tttgctccaa atggcaaaat atacacaaca ctctttatag caaatataag
     1381 tggacaggta ttcaatggcg gaaggaaaag caatgtcaga atttttgcct ttttcgcgac
     1441 cagcaatggg cgtggaggaa ctcgctgcag ttaaagaggt tctcgaatcc ggttggatca
     1501 caaccggtcc gaagaatcag gcgcttgaac aagccttttg ccagttgacg ggaaatcagc
     1561 atgccatcgc ggtcagttca gccaccgccg gaatgcatat cacgctaatg gcgttggaaa
     1621 ttggcaaggg cgatgaagtg attacgcctt ccctgacctg ggtttcaacc ctcaatatga
     1681 tttccttgtt gggtgcaacg ccggtaatgg tggatgtcga ccgcgatacg ctgatggtca
     1741 cgcctgaagc gatcgagtca gccattacgc cacgcactaa agccatcatt ccggtgcatt
     1801 atgccggtgc gccagcagat attgacgcca ttcgcgccat tggcgaacgt tacggcatcg
     1861 cagttatcga agatgctgcc catgccgtcg gtacgtatta caaagggcga catattggcg
     1921 caaaaggtac cgctattttt tcgtttcacg ccatcaaaaa tattacctgt gcggaaggtg
     1981 gcctgattgt tacagataat gaaaaccttg cccgcctgct acggatgctg aaatttcacg
     2041 gtctgggtgt cgatgcctat gacagacaaa cctggggccg tgcaccgcaa gctgaagtct
     2101 taacgccggg ctataagtat aatctgaccg atattaacgc cgcgattgcc ctgacccagt
     2161 tagccaaatt agagcacctc aacacccgtc ggcgcgaaat tgctctgcaa tatcagcaag
     2221 cactggcagc tctccccttt cagccattaa gccttcccgc ctggccgcac gttcacgcct
     2281 ggcatctgtt tattattcgt gtcaatgaac aacgttgtgg tatcagtcgc gatgcgttga
     2341 tggaagcgtt aaaagaaaga ggcattggta ccgggttaca tttccgcgcc gctcacacac
     2401 aaaaatatta tcgcgagcgt tttcccacgc tgtcgttacc gaataccgaa tggaatagcg
     2461 aacgcatctg ttcgttgccg ctgttcccgg atatgaccac cgccgatgcc gaccgcgtca
     2521 tcacagccct tcagcaactc gcaggacaat aagccatgtt tgaaatccac cctgttaaga
     2581 aagtctcggt ggttattccc gtttataacg agcaggaaag cttaccggaa ttaatcaggc
     2641 gcaccacaaa agcctgtgaa tcactgggga aagagtatga gatcctgctg attgatgacg
     2701 gcagtagcga taattccgcg catatgctgg tcgaagcctc acaagcggag aacagccata
     2761 ttgtgtctat tttgcttaac cgcaattacg ggcaacattc agcgattatg gcgggattca
     2821 gtcacgttac tggcgactta attattaccc ttgatgccga tctccagaat ccgccagagg
     2881 agatcccccg cctggtggca aaagccgatg aaggctacga cgtggtagga actgtgcgtc
     2941 agaaccgcca ggacagctgg tttcgtaaaa cggcatcgaa gatgattaac cggcttattc
     3001 agcgcaccac cggcaaagcg atgggtgact acggttgtat gctgcgcgcc tatcgccgtc
     3061 atattgtcga tgcgatgttg cactgccatg aacgcagcac ctttatcccg attctggcga
     3121 atatcttcgc ccgccgtgcc attgaaattc cagtgcacca tgccgagcgt gagtttggtg
     3181 aatccaaata cagttttatg cgcctgatta atttgatgta cgacctggtg acctgcctta
     3241 ccacaacgcc gctacgtatg ctgagtctgc ttggcagcat tattgcgatt ggaggtttta
     3301 gcattgcggt gctgctggtg attttacgcc tgaccttcgg accacaatgg gcggcagaag
     3361 gcgtctttat gctatttgcc gtgctgttta cttttattgg cgctcagttt atcggcatgg
     3421 gattgctcgg tgaatatatc ggcaggatct acaccgatgt ccgcgcccgc ccccgctatt
     3481 ttgttcagca agttatccgt ccatccagca aggaaaatga ataatgaaaa ccgtcgtttt
     3541 tgcctaccac gatatgggat gcctcggtat tgaagccctg ctgtctgccg gttacgaaat
     3601 tagcgccatt tttacccata ccgataatcc cggtgaaaaa gccttttatg gttcggtggc
     3661 tcgtctggcg gcggaaagag gcattccggt ttatgcgccg gataacgtta atcatccgct
     3721 gtgggtggaa cgtattgccc aactctcacc agaggtgatt ttctcttttt attatcgcca
     3781 tcttatttgc gacgagattt tgcagctcgc tcccgcaggt gcatttaatc tgcatggttc
     3841 gctgttacca aaatatcgtg gtcgcgcgcc gctgaactgg gtgctggtga acggtgaaac
     3901 ggaaactggc gttacattgc accgaatggt gaaacgtgcc gatgccgggg ccattgtggc
     3961 gcaactgcgc attgccattg cgccagacga tatcgctatt acgctgcatc ataaattgtg
     4021 ccatgccgcg cgccagctac tggaacagac attacccgcc attaaacacg gtaatattct
     4081 ggaaatcgcc cagcgcgaaa acgaagccac ctgttttggt cgcagaacac cggaagacag
     4141 tttcctcgaa tggcataaac cggcatccgt gctgcacaac atggtgcgtg ccgttgccga
     4201 tccgtggccg ggtgccttca gctatgttgg taatcagaaa tttaccgtct ggtcgtcacg
     4261 cgtgcattct catgcgcccg cagcacaacc ggggagcgtg atttctgttg cgccactgct
     4321 gattgcctgt ggcgatggcg cgctggaaat cgtcaccgga caggcgggcg acggcattac
     4381 tatgcagggc tcgcaattag cgcagacgct gggcctggtg caaggttcac gcttgaatag
     4441 ccagcctgcc tgtgccgccc gacgccgtac ccgggtactc atcctcgggg tgaatggctt
     4501 tattggcaac catctgacag aacgcctgct gcgcgaagat cattatgaag tttacggtct
     4561 ggatatcggc agcgatgcga taagccgttt tctgaatcat ccgcattttc actttgtcga
     4621 aggcgatatc agtattcatt ccgaatggat tgagtatcac gtcaaaaaat gtgatgtcgt
     4681 cttgccgctg gtggcgatag ccacgccgat tgaatatacc cgcaacccac tgcgcgtatt
     4741 tgaactcgat tttgaagaga atctgcgcat tatccgctac tgcgtgaagt accgtaagcg
     4801 aatcatcttc ccgtcgactt cagaagttta tgggatgtgt agcgataaat acttcgatga
     4861 ggatcattct aatttaatcg tcggcccggt gaataaacca cgctggattt attcggtgtc
     4921 taaacaatta cttgatcggg ttatctgggc ctatggcgaa aaagagggtt tacagtttac
     4981 cctcttccgc ccgtttaact ggatgggacc acgactggat aaccttaatg cggcacgaat
     5041 cggcagctcc cgcgctatta cgcaactcat tctcaatctg gtagaaggtt caccaattaa
     5101 gctaattgat ggcggaaaac aaaaacgctg ctttactgat atccgggatg gtatcgaggc
     5161 gttgtaccgc attatcgaaa atgcgggaaa tcgctgcgac ggcgaaatta tcaacattgg
     5221 caatcctgag aacgaagcga gcattgagga actgggcgag atgctgctgg cgagcttcga
     5281 aaaacatccg ctgcgccatc acttcccacc gtttgcgggc tttcgtgttg tcgaaagtag
     5341 cagctactac ggcaaaggat atcaggacgt agagcatcgt aaaccgagca tccgcaatgc
     5401 ccgccgctgc cttgactggg aacccaaaat tgatatgcat gaaaccatcg acgaaacgct
     5461 agatttcttc ctgcgcaccg ttgatcttac ggataaacca tcatgaccaa agtcggctta
     5521 cgcattgatg tcgatacctt tcgtggcacc cgtgaaggcg tgccgcgtct gctggaaatc
     5581 ttgagtaagc ataatattca ggccagcatt tttttcagcg tcggcccgga caatatgggc
     5641 cgccatctct ggcgactggt gaagccacag tttttgtgga agatgctgcg ctcaaacgcg
     5701 gcatcgcttt atggctggga tattttactg gcaggtacgg cctggccggg taaagagatt
     5761 ggtcatgcca atgccgatat cattcgtgaa gcagctaaac atcacgaagt cggcctgcac
     5821 gcctgggatc accatgcctg gcaagcccat agcggtaact gggatcggca aacattggtc
     5881 gacgatattg cacgcggcct tcgcaccctg gaagagatca taggtcaacc ggtaacctgt
     5941 tctgccgctg cgggctggcg tgccgaccag caggtgatcg aagcaaaaga agcgttccat
     6001 ttgcgctaca acagcgattg tcgtggggcc atgccgtttc ggccactgct cgaatcagga
     6061 aaccctggca ctgcgcaaat tccggtgacc ttacccacct gggatgaagt gattggtcgg
     6121 gatgtgaaag cagaagattt taacggttgg ttactcaacc gcatcctgcg agataaaggc
     6181 atgccggttt ataccattca tgcagaagtt gaaggctgcg cttatcagca taattttgtg
     6241 gatctcctga aacgggcagc ccaggaaggc gtgacatttt gccctttaag cgaactgtta
     6301 tcagggacgt tgccgctcgg acaagttgtt cgcggaaata ttgccggacg tgaaggctgg
     6361 ctgggttgcc aacaaattgc gggtagtcgt tgatgaaatc ggtacgttac cttatcggcc
     6421 tcttcgcgtt tattgcctgc tattacctgt taccgatcag cacgcgtctg ctatggcaac
     6481 ccgatgaaac gcgttatgcg gaaatcagtc gggaaatgct ggcatccggc gactggattg
     6541 tgccccatct gttagggcta cgttatttcg aaaaacccat tgccggatac tggattaaca
     6601 gcattgggca atggctattt ggcgcgaata actttggtgt gcgggcaggc gttatctttg
     6661 cgaccctgtt aactgccgcg ctggtgacct ggtttactct gcgcttatgg cgcgataaac
     6721 gtctggctct actcgccgca gtgatttatc tctcattgtt tattgtctat gccattggca
     6781 cttatgccgt gctcgatccg tttattgcat tctggctggt agcgggaatg tgcagcttct
     6841 ggctggcaat gcaggcacag acgtggaaag gcaaaagcgc gggattttta ctgctgggaa
     6901 tcacttgcgg catgggggtg atgaccaaag gttttctcgc ccttgccgtg ccggtattaa
     6961 gcgtgcttcc atgggttgca tcgcaaaaac gctggaaaga tctctttatt tacggctggc
     7021 tggcggttat cagttgcgta ctgacggttc tcccctgggg actggcgata gcgcagcggg
     7081 agcctgactt ctggcactat tttttctggg ttgagcatat tcaacgcttt gcaatggatg
     7141 atgctcagca tagagctccg ttctggtact acttgccggt catcattgcc ggtagcctgc
     7201 cgtggctggg attactcccc ggagcactgt acgcaggctg gaaaaaccgc aagcattccg
     7261 caaccgtcta tttgttgagc tggacgataa tgccgctgct gtttttctcc gtcgctaaag
     7321 gcaaattgcc cacctatatt ctttcctgct ttgcacctct ggcaatgctg atggcgcatt
     7381 acgctttgct ggcagcaaaa aataatcctc tggcgctgcg gattaatggc tggattaaca
     7441 tcgcttttgg tgtcactggc attattgcca catttgtggt ctccccatgg ggaccaatga
     7501 acacgccagt gtggcaaacc ttcgagagct ataaagtctt ttgtgcctgg tcgatttttt
     7561 cgctatgggc atttttcggc tggtacacct taacaaacgt cgaaaagacc tggtcttttg
     7621 ccgcgctttg cccgctgggg ctggcgttgc tggtaggatt ttcaattcct gacagagtca
     7681 tggaaggaaa acatccgcaa ttttttgtcg agatgacaca agaatcactt cagccaagcc
     7741 gctatattct tactgatagc gtcggtgttg ccgcaggtct ggcatggagc ctgcaacgcg
     7801 atgacatcat catgtatcgc cagacaggtg agttgaaata cggccttaat tatccggatg
     7861 cgaaagggag atttgtcagc ggtgatgagt tcgcaaactg gcttaatcaa catcgtcagg
     7921 aggggattat tacactcgtg ctttcggttg accgcaatga agatatcaac agtctcgcca
     7981 ttccgcccgc agatgccatc gatcgtcagg agcgtctggt gctgattcag tatcgtccca
     8041 aatgatctgg ctaacattag tctttgccag cttacttagc gttgccgggc agttgtgtca
     8101 gaaacaggca acctgctttg tggcgataaa caaacggcgc aaacatatcg tgctgtggct
     8161 gggactggcg ctggcttgtc ttggtcttgc catggtgctc tggctgctgg tcttgcagaa
     8221 cgtaccggta ggcattgcct acccgatgtt aagtctgaat tttgtctggg tgacgctggc
     8281 tgcagtaaaa ctgtggcacg aaccggtatc gccgcgtcac tggtgtgggg tggcgttcat
     8341 tattggtggc attgtgatcc tcgggagtac cgtgtaatgg gcctgatgtg gggattattc
     8401 agcgtcatta tcgcctccgc tgcgcaatta agcctgggtt ttgcggcgag tcatctgccg
     8461 ccgatgacgc acttatggga ttttatcgcg gcgctgttag cttttggcct ggatgcccgg
     8521 atactgttac tcgggctgct gggttatctg ctttccgttt tttgttggta taaaacacta
     8581 cataaacttg cactaagcaa agcctatgcc ttgctcagta tgagttatgt gctggtatgg
     8641 attgcttcaa tggtacttcc cggctgggaa gggacttttt cgctgaaagc actactggga
     8701 gtagcctgta ttatgagcgg gttgatgctg attttcctgc ccacgacaaa acaacgttac
     8761 tgagttttcc cctgccactt tacactgccg ttcccactca tcggggctat aactgcttgc
     8821 cgagaggact ttcaccgtat gcagcttttc acgattaata caataaagcg cattctgcaa
     8881 aggcgagagt aaatccccca ctttcacggc gaagtcactc ttcacttcgg cgatcatttt
     8941 tatcgtgcca ccagcatcac aaagcttaag tacatgtctg ttatcttgtt tatcacagca
     9001 tgacttttta accagccatt ccattgcacc accctgcttg ataagaattt tcacattaat
     9061 ttacagatta acagataaac ctattatcaa cggcaatgga atggcaaaat tgcgaagtga
     9121 aactaatgat ttcttaatcc tggcgttgca cccactcttt tagcgcctga cgtgaaatct
     9181 taatgccgcc gtttttcagt tcaggcggca gcgttaacca acgcaccggt tgctgaaaac
     9241 gggccagctt atctttcacc cattcactaa gatcaacggt ttgctggtca tactccacca
     9301 ccgctaccgg ccgatgacca aactccttgt cggcaacggg gacgataaac acctgcaaaa
     9361 ccgcaggatg tgcagcaata acgcgctcga cttcttccgg ctgaataccc tcaccgccac
     9421 tgaagaacag attgtctaaa cgcccgacaa tggtcagctt gccattatgc agctcaccgc
     9481 ggtcgcgcgt agcgtaccag ccttcgtcat taaccagtgc caccagttgc ccgttacgcc
     9541 agtaaccttc agccatactg gcagcccgca gccacacttc atcattaacg attttcactt
     9601 cccgacccgg cagcggcgaa ccaacgtctg ccagaccgtc ggcttctttc gcacacaccg
     9661 tggaggcaaa ctcggtcaga ccatagccgc aaaagcaacg aatcccctgc tcgcgcgcct
     9721 gttccgtcaa ctcgaccggg atagccgcgc cgccaagtag caccgctttc agggaaacgg
     9781 aactacggtt aaccagcaaa cgccagagtt gtgttggcac cagtgaagcg tgagtacagc
     9841 ctgccagcat ttgctccaat ggctgtttat ctcgtaccgt catccgcgca ccagcgtata
     9901 accagcgcca cataattccc tgaccggaga cgtgaaacag cggtaaagag agcaaccagt
     9961 catcgtgctc gccaaacggt atcagcgata acacaccttg cgcactggca agatgggcct
    10021 gataagtatg aacagcggct ttcggcaaac cggtagagcc ggaggtcaac gtcattgagc
    10081 acagacgcgt cggctgccac gcagcggcat gtgcgccttc aaccagctga atgtgcagcg
    10141 acgttaacgc cggaaacgtg ttttccccat ccggcaccag cgcaaattgc agcgtcagat
    10201 tgggcagcaa ttcttcaagc aacggttgcg gcagctgagg gttcacgggc agcacccgcg
    10261 ccccgcattg cagtaacgcc agccaggcga gcagcgtttg cggcgtattc cacgcccgca
    10321 acatcacgcc gctgccctca acaacgtcct gcgccgcaaa gccggaggct aattcatcga
    10381 cgcgagcaca aagctcgcgc cagttaagtt gctcgtcatt aagacgtaag gcgatggctt
    10441 ctccccgcac ttgccgccag tgacgccacg gccagtcaga gaagatcata acagccgctc
    10501 cagtgcatca acttccacta acggtagcgg gctacccggc cagcgacgaa cctgctgcgc
    10561 ctgcatcaga tccagcgtgt ccagccccgg aatggtgtct ggcgttaacc aggcggcaat
    10621 ccgcgccagt tgcgttaagc ctaagctcga ctcaatagaa gaactgatca ccgccgtcag
    10681 ccccaacgcg tgcgccgcct gcacctgctc gcgtacttta tccaggctgc ccgtgagcgt
    10741 gggcttgata accaccgcgc gcacgccctc ttcagctaca aaggcaaaat ctggctcgcg
    10801 caggctttca tcccaggcaa tggcaatgcc ggtttcacgg gcaaacgctc gcgaatcatc
    10861 gcgggttttg cacggctctt cgagaaaagc aatgcggtgg cgataatccg ggttaacgta
    10921 tttggcaaac tgctggcctt tccgcggcgt ccaggcgcgg ttcgcgtcaa gacgcagatg
    10981 cagatccgga attgcctcca gcaacagatt gaccaccatg ccgtcgcgca cggcttcgta
    11041 caatcccact ttgaccttcg ccactttctc gccaggcata tcggcaagtt tgaggatcag
    11101 atcgtccgga tcgccattac acagcggtgc cgcacggtag ttggctgctt gcggcaacgc
    11161 atcggtcagt tctgccaatg cacagcttac gccaaaggcc acggaaggca tctgcggtag
    11221 ctcgcaatcg cctgccagcc agttatttac ccaggcaagc agcacgcttt gcgcctcttc
    11281 ccaggtttcc tggctgaagc ccggcagtgg ggagatctcc ccccaccctt cgcgctcgcc
    11341 ctcacgcagg caaacgtaca gcccgtcgcg ggtttttaac cgcctgtcgc gcagaaccac
    11401 ccccgcgtcc atggggatct gccagcggta tacctgcgcg ctacgcatta cggattccgt
    11461 ttgaatttgc tgaagtcagg ctgacgtttc tggttgaaag cgttgcgacc ttcctgacct
    11521 tcttccgtca tatagaacag catggtggcg ttacctgcca gctcctgcag acctgcctga
    11581 ccgtcacagt cggcgttcag tgcagctttc aggcagcgca gcgccatcgg gctgttttgc
    11641 agcatttcgc ggcaccaacg gacggtttct ttttccagat ccgccagcgg taccacggtg
    11701 ttcacaaggc ccatatccag cgcctgtttt gcgtcgtact gacggcacag gaaccagatt
    11761 tcacgcgctt ttttctgccc gacgatgcga gccatgtagg aagcgcccca gccgccgtcg
    11821 aaggaaccga ctttcgggcc agtctgaccg aagatggcat tatctgccgc gatagtcagg
    11881 tcgcacatca tgtgcagaac gtgaccgccg ccgatggagt agccagccac catcgcgaca
    11941 accggtttcg gacaggtacg gatctgacgc tggaagtcca gcacgttcag gtgatgtacg
    12001 ccggaatcat ctttatagcc gccgtaatca ccacgcactt tctggtcacc accggagcag
    12061 aacgctttat cgcctgcacc agtcagaatg atcacgccga tgttgtcgtc ataacgcgca
    12121 tctgccagcg cctggatcat ctcttttacc gtcagaggac ggaaggcatt gcgcacctgc
    12181 ggacgattaa tggtgatttt tgcgataccg tcggtggatt tttcataacg aatgtcctcg
    12241 aaaccttcgg agcagtcgtg ccattcaacc ggtgcgtaaa gcattgcttc atcaggataa
    12301 atcatattgt gtcctttagt cagaaacgca agatctgcgc cagacttgcg attacgccag
    12361 cgggattttc ccgatgcgcg ttatgtccgg cgcgaggaat gacatggcat tcggcagcca
    12421 gttccgccgc cagggcgcgg aatttgctgt cacgttcacc acataaataa taaaacgcaa
    12481 atgtgcgggc gctaaggtta gcacgtaaat cgggctggac ggcgagagaa gtcgcctcca
    12541 gcatggcggc aagcgttgcg ccattattgt tgctgcgcag cgcaaccagc tcccggcgtt
    12601 gttcgtcatt gagtgaggca aaaacaggct gttgatacca gtcagcaaat accgccgtta
    12661 acggttctgt gcgaaaacgc tgcgcccatt ggcgatcgga acgctgacgt tccgtgcgtt
    12721 gttcagcatt ttgcaaccca gggtgcccgc cttcgacaat aaccccacaa agccccggca
    12781 tcccctgaca agccgccatc atcgccaccc gtccgccaag cgagtacccc accagccaga
    12841 agttaaggat gttgtaacta accagagttt tgcgcagtaa gccggtgaca tcatcaaatc
    12901 catcgacgcg aatcgccgcc gaaccaccgt gacctgggag atcaacatac aaccgcgagt
    12961 agttggcaaa cacctcgccc acttcttgcc attcatggca atcgccggaa aaaccgtgca
    13021 aaaacaccag ccagggtaaa cctggtttac cgtgttttgc ctgcgcgtgc aggatcataa
    13081 atggctaacc tgcgccagca gttgctggag ggtttgcgca ccattggtgt cgttaaccac
    13141 catttcaatt accgtggtgg tcggcgtgcg ccaggcgtcg gcaagtgccg tttcaagttc
    13201 ctgccagttt tgcggacgat gatatttcag ctcgaacatc gcggcggcgt gctcaaaatg
    13261 gacgttttgc ggcatcagat agaaacgctc gcgctcgttt ttcggcgttg gcaacagcga
    13321 gaaaatttgc ccgccattgt tgttcaccac aattaatacc agcggcgcgg aaacctgacg
    13381 taataacgcc agcgcgttga gatcgtaaag tgctgagaga tcgcccacaa tcgccagcgt
    13441 cggtttgcca ctggcccgct gaacgccagc agcagttgag agcagcccgt cgataccgct
    13501 ggcacctcgg ttgctgtaca ccgggtaacc tgccggaagt tgcgaaagcg catcaatcag
    13561 acgtaccacc aggctgttac cgacaaacaa ttgcccctgt tcaggcaaat agtcgctgat
    13621 gcgatgcgcc agttgcgctt cgccaaacgc atcgcgacgg gcaataaccg cctgcattgc
    13681 ctgttcagcc aagcgcggga tttcaacgca ccagggctgg cgtttttctg ccggatgttg
    13741 ctccagccag tcggcaatat tggcaattaa gcgacgtccg cgatggtgtg ccggatcaag
    13801 tcgcccttca atgtcatcaa caatccagta ctcttcgggt tcacagcttg cctgccattg
    13861 caggagccgt ttgcccgtca ggctgcttcc cagttgcacc acaatttgcg cctgttgcag
    13921 ttcgctggtc gctttggcat tgcctaacca gagatcggca cacggcagcg gctgtccggt
    13981 ttgcgacagc acgtcaccga tcagcggcca gccaagagtt tgcgcccaca gtgcgacttt
    14041 tttgccctct tccgcactca tgcgcccggc aaccaccacg ccgcgctttt gtcgccagaa
    14101 gaaccagtcg cgctgttttt cactttccag acgaggcgct tcacgcagcc acggtttgtc
    14161 gtcatgccac cagtcgccca gccgctgttg ccagctaagc ccggtgtcgt ccatttcgcc
    14221 atacagcggt tcagcaaacg ggcagttgat atggactcca ccagcatgaa gcgtaccgag
    14281 agcgtggtcg atggtagaaa ccagccaacg tgcggggata tcctgggtcg ggcgcggcag
    14341 tgaaatactg tgcgtggggt gagaggcgaa cattcccggc tgacgaatcg cctgattcgc
    14401 gccgcagtca attagctccg gcgggcgatc ggcggttaac aggatcagtt tttctccggt
    14461 taacccagct tcaatcagtg caggatagag atttgctacc gccgtgccgg aggtcacaat
    14521 caccgccacc ggctgcttgc tgactttcgc cagccccagc gccagatgcc ccagtccacg
    14581 ctcatcgaaa tgggtgtggt gaatgaatgc ggaattctcc gccgccgcta acgttaacgg
    14641 tgtagaacgc gagccagggg cgatacagat gtgtcttacg ccgtgacgcg ttaatgcttc
    14701 cagaatgacc gccgcccagc gtcggttaaa tgcgcttact gacatgagtt tgtccggtat
    14761 caatattgcg gctaagtata aggagctaca aaaatagatt attgatatga atcggtaatg
    14821 atgcgactca ttactattcc atttgtaata aagtacgcag ccctgccgct ttgttgtcga
    14881 tttcctgcca ctcttgctcg gggtcggaac cacggacaat gcccgcgcca gcatatagtc
    14941 gcacgacatt gccactgatt tttgctgagc gcagggaaac gcagaattcg ctttgttgta
    15001 aagagagata gcccgcagaa ccggcgtacc attctcgggt gaacggttcg tgacgggcga
    15061 taaactgtcg cgccagatcg cgcggtaagc cagcaactgc tgccgttggc tgcaactgat
    15121 gtaaacagat cacatcatcc gctttgttga gtgaagtcca gatacagcgg cgaagatgct
    15181 gcactttacg cagacgcagt acctgcggcg gtaaaacatc cagcgtttgg gtatcggcct
    15241 gtaatcgttg gcagatatct tccaccacca acatgttctc acgctggttt ttatcatccg
    15301 ccatcagcca ctcacccaac cgctgcgcct gcttatcatc aggatgattt gctaccgttc
    15361 ccgccagcgc ttcagtacgc agcgctttgt cacgccgccg ccatagccgt tccggtgaag
    15421 agccaagaaa agcattttcg ccatcaaagg ccatataaaa atgatagcag ttcagattaa
    15481 gccgacgact ggcagccatc atcgctgccg cattgaccgg acttgcgaaa tgcaggtcag
    15541 ttgcccgagc aagcaccact ttgtcgagct caccttcggc gatggttttc gttgccagtt
    15601 cgattaattg cgtccacccc gttttgtccg gccagtgttg ttctcgggtg gtggttaaat
    15661 gtaacccagg caagggcttg atactcacca gtgtggcgat aaattctttt gccttaatcg
    15721 catcatgctg aagggagctt tcgctgaata gcgtcagcct cagcgtggct ttaccgccac
    15781 agcgtcgcca ttccaggcgt ggtaaaagta aattgccctg cgacgggtca aacgcattca
    15841 gcccccagat gcgtaagtcg gcgtgtttcg ggtgctgacg aagaaacgtt tgtgcctggt
    15901 ccaacgacgt aaaacgggta atcgcgccca ggacggcagc ttcttcatca ccattacgtt
    15961 gttgccagta gaattgcggg tacgtttgct gactggccag ccagctcaag gcatcaaaag
    16021 cgtctttgag agggaaagga atatcaataa cccgaatgcc gggtgtcgcc ggaatctctt
    16081 gcgacaaatg gcgcagtaga ttttccagcg ccgtagtaag tgattgcacg cggacctctc
    16141 cctgttaaaa gcctcacatt atacggggta ctacaaaaaa aatgcagtac cccggtgtag
    16201 ggaggtttta acggcgtgcc agcaacagtc ctagtaccag cccaacggcc gcgcccacac
    16261 caattccttg ccagggtttt tcgtggacgt agtcatcagc acgataaaca gcctgcttcg
    16321 cccgatagta ataactgtct gaagcctggc taacccgttt tttcacatca tccagcgctt
    16381 tttctgcacg cgctttcagc tcaacatatt tctgatcggc gggatcgcca gaggagcgga
    16441 gcacctcttc cagtgtttca ctaagcagcg tcaggtcatc atcgatacgt gtatcaccaa
    16501 actgattaga cattctcgtt ctccatttgc gtaaaacctg tcactaacta tagacaaggg
    16561 ttattgatta cgcctgaatt acctcgcgtg ccatcccaat gtgtgggata ccatcttctt
    16621 catagacctc cgtcacaggg ataaagccaa aactctggta gaagttttgc agatgcgcct
    16681 gcgccccgag gtaaacaggt ttatcaggcc agtgacgcgt acatgtttcg agtgttttat
    16741 tcatcagctg ctgccccact ttttcgccac gcaacgcttc actaacaatc acccgaccaa
    16801 taacgaccgg ctcaagatca tcatcacttt tcagaatcct cgcatacgcc accagttcat
    16861 catttttcca accgagaata tggcgattat cccccgtcaa atcatcacca tcgatatcct
    16921 gataagggca attctgctca acaacaaaca ccgcgcaacg cagttgtaat aaggcataaa
    16981 gctgggaaac cgataattca gagtgatgca gatcttgcca ttcaatcata atgctctcct
    17041 tttagatgta tgccttatac tagccggaat attttttgaa acgtgatgaa ctcatggaat
    17101 taattttttt aggtacttca gccggtgtgc caacccgtac gcgcaatgtc acggcaatat
    17161 tgcttaacct gcaacatccg acccagagcg gtctttggtt gtttgactgc ggtgaaggca
    17221 cccagcatca gctactgcat accgccttta accctggaaa actggacaag atttttatca
    17281 gtcaccttca tggcgatcat ctctttggtt tacccggctt gctgtgcagt cgttctatgt
    17341 ctggtattgt ccaaccctta acgatttatg gtccgcaagg tatccgtgaa tttgtggaga
    17401 ccacgctgcg gattagcggc tcatggaccg attatccgct ggaaattgtc gaaattggcg
    17461 ctggcgaaat tttcgatgat ggcctgcgca aagtgaccgc ttatccgctg gaacacccac
    17521 tggaatgcta tggctatcgt attgaagaac atgataaacc gggtgcatta aatgcccagg
    17581 cattaaaagc tgctggcgta ccgcctggcc cactgtttca ggaattaaaa gcgggcaaaa
    17641 caatcatgct ggaagatgga aggcagatta acggcgcaga ttacttagcc gctccagtgc
    17701 caggtaaagc gctcgctatt ttcggcgata ccggcccctg cgatgccgca cttgacctgg
    17761 ctaaaggtgt cgatgtcatg gtgcacgaag cgacgctgga tataaccatg gaagccaaag
    17821 ccaatagtcg cggccacagc tctacacgcc aggctgcgac actggcccgt gaggctggag
    17881 tcggtaagct aatcattacc cacgtcagct cgcgctatga tgacaaaggt tgtcagcacc
    17941 tgttacgtga atgcaggtca attttcccgg cgactgaact ggcgaatgat ttcaccgtgt
    18001 ttaacgttta actctgctta ttattaaaaa cagggcgaaa cttgccctgt tatcgcaacc
    18061 cgcgctacat atatcccccc acacgggcat attgagcgat ttttgatatt tattcatctc
    18121 cttacgcact atattcagag caataagttg cctcttttta tcttcattca gcacaacctt
    18181 tgcaactaaa aggagcctat gatggttaca gttgtcagca attattgtca attatctcaa
    18241 acccaactct gtcagacatt tgcagaaaaa tttactgtga ccgaggaatt actgcagtct
    18301 ttaaaaaaaa cagcgttatc cggagatgaa gaaagtattg agttactgca taacattgcg
    18361 ttaggttatg atgaatttgg gaaaaaagct
//
LOCUS       DACBVI010000090        18032 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.090, whole genome shotgun sequence.
ACCESSION   DACBVI010000090 DACBVI010000000
VERSION     DACBVI010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.090"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            297..1196
                     /gene="yegS"
                     /locus_tag="IU325_14810"
     CDS             297..1196
                     /gene="yegS"
                     /locus_tag="IU325_14810"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HAP5810277.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VRIPEALDKALKLAIAGDAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1278..2057)
                     /locus_tag="IU325_14815"
     CDS             complement(1278..2057)
                     /locus_tag="IU325_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP5810278.1"
                     /translation="MTMNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRF
                     LEQKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMT
                     VILDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMH
                     GSIAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKF
                     NRRGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2157..3197)
                     /gene="gatD"
                     /locus_tag="IU325_14820"
     CDS             complement(2157..3197)
                     /gene="gatD"
                     /locus_tag="IU325_14820"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP5810279.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(3246..4601)
                     /locus_tag="IU325_14825"
     CDS             complement(3246..4601)
                     /locus_tag="IU325_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol permease IIC component"
                     /protein_id="HAP5810280.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(4605..4889)
                     /gene="gatB"
                     /locus_tag="IU325_14830"
     CDS             complement(4605..4889)
                     /gene="gatB"
                     /locus_tag="IU325_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HAP5810281.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(4920..5372)
                     /gene="gatA"
                     /locus_tag="IU325_14835"
     CDS             complement(4920..5372)
                     /gene="gatA"
                     /locus_tag="IU325_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HAP5810282.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(5382..6644)
                     /gene="gatZ"
                     /locus_tag="IU325_14840"
     CDS             complement(5382..6644)
                     /gene="gatZ"
                     /locus_tag="IU325_14840"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HAP5810283.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDTANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(6673..7527)
                     /gene="gatY"
                     /locus_tag="IU325_14845"
     CDS             complement(6673..7527)
                     /gene="gatY"
                     /locus_tag="IU325_14845"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HAP5810284.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(7837..8889)
                     /gene="fbaB"
                     /locus_tag="IU325_14850"
     CDS             complement(7837..8889)
                     /gene="fbaB"
                     /locus_tag="IU325_14850"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HAP5810285.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            9145..10422
                     /locus_tag="IU325_14855"
     CDS             9145..10422
                     /locus_tag="IU325_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5810286.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAALMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWAGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10419..11423
                     /locus_tag="IU325_14860"
     CDS             10419..11423
                     /locus_tag="IU325_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAP5810287.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGCIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLERKGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGINAIDPALKAELDAVNQLDFNRYATALAKYRQQREVV
                     "
     gene            11420..12385
                     /locus_tag="IU325_14865"
     CDS             11420..12385
                     /locus_tag="IU325_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP5810288.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(12359..13105)
                     /locus_tag="IU325_14870"
     CDS             complement(12359..13105)
                     /locus_tag="IU325_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HAP5810289.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(13157..13975)
                     /locus_tag="IU325_14875"
     CDS             complement(13157..13975)
                     /locus_tag="IU325_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HAP5810290.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYSYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFHGTVEELQAFVDGIKETP"
     gene            complement(14040..14840)
                     /gene="thiD"
                     /locus_tag="IU325_14880"
     CDS             complement(14040..14840)
                     /gene="thiD"
                     /locus_tag="IU325_14880"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP5810291.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTQNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(14837..15625)
                     /gene="thiM"
                     /locus_tag="IU325_14885"
     CDS             complement(14837..15625)
                     /gene="thiM"
                     /locus_tag="IU325_14885"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HAP5810292.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAIEQAKRSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENIASACHWMKQAGERAVASSEGPGSFVPHFLDALWQLTPEVQA"
     regulatory      complement(15660..15756)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15848..16120)
                     /gene="rcnR"
                     /locus_tag="IU325_14890"
     CDS             complement(15848..16120)
                     /gene="rcnR"
                     /locus_tag="IU325_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HAP5810293.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            16241..17065
                     /gene="rcnA"
                     /locus_tag="IU325_14895"
     CDS             16241..17065
                     /gene="rcnA"
                     /locus_tag="IU325_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux transporter RcnA"
                     /protein_id="HAP5810294.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            17284..17622
                     /gene="rcnB"
                     /locus_tag="IU325_14900"
     CDS             17284..17622
                     /gene="rcnB"
                     /locus_tag="IU325_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HAP5810295.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(17704..>18032)
                     /locus_tag="IU325_14905"
     CDS             complement(17704..>18032)
                     /locus_tag="IU325_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP5810296.1"
                     /translation="LGQQNQTSRKMQVYLSSSDLWGSSNTILKGAEDNGVGFILESNG
                     SPVTLLNITNSSKGYTNLKEVAAKSKLTDTTVSIPITASYYVYDTNKVKSGALEATAL
                     INVKYD"
BASE COUNT         4434 a         4645 c         4355 g         4598 t
ORIGIN      
        1 taggaattac aaacatgaag ccctgcccag gtatccccat gagggcttta atatatttag
       61 aataagatag tgaagatgcc actgtttagt tgaatattag gtatatgctc ttttttgaaa
      121 tttaccggtg gctaccgtta acattcactg tccccattgc aagctcctgg tggtaaccac
      181 tgaatcctcc atacttgaac tgatttttta tcctccgact ttcatcctgt tctgactcca
      241 ccttttcttt tctgctctac actatctaca gaccaatcat aaaggcacat acgatcatgg
      301 cagaatttcc cgccagctta ctgattctta atggcaaaag tactgacaat ttaccattgc
      361 gcgaagcaat tatgctgttg cgtgaggaag gaatgacgat ccatgtgcgg gtcacctggg
      421 agaaaggcga tgccgcacga tatgtagagg aggcccggaa gttgggcgtc gcaacggtga
      481 ttgccggtgg tggcgatggc accattaatg aagtttctac ggcgttgatt cagtgtgagg
      541 gggatgacat acccgcgcta ggaattttgc cattaggaac cgccaatgat tttgccacca
      601 gtgtaaggat tcctgaggca ctggataagg cgctgaaact ggcaattgcc ggtgacgcca
      661 ttgcgataga tatggcgcag gtcaacaaac aaacctgttt tattaatatg gcgacaggcg
      721 gatttgggac gcgtattacc acagaaacgc cggaaaaatt aaaagccgcg ctgggtggcg
      781 tctcttacat cattcatggc ttaatgcgca tggatactct gcaaccggac cgttgtgaga
      841 tccgcggtga aaactttcac tggcaaggtg acgccctggt cattggtatt ggtaacgggc
      901 gtcaggccgg tggcggtcag caattgtgcc cgaacgcgtt aattaacgat ggcttgctgc
      961 aactgcgcat ttttaccggc gatgaaattc ttccggctct cgtatcaaca ttaaaatctg
     1021 acgaagataa cccgaatatt atcgaaggcg cttcgtcgtg gtttgatata caagccccac
     1081 acgaaatcac ttttaatctt gatggcgaac cgttgagcgg acaaaacttt catattgaaa
     1141 tacttccggc ggcgttgcgt tgtcgattac caccagattg cccattgttg cgttaatcac
     1201 actattctct cttgaaccag ggagaatccg taatgccgga tgcggtgtaa aaactttatc
     1261 cggcatacag agcaatacta caccgtaatt aatttcaccc gtgtcttctg caatgccagt
     1321 ttatccacct ctgacacggc atcatcggta ataataatgt caattttttc gattggcagt
     1381 acctgattaa aaccgcgacg attaaatttc gatgaatcga gaacggcaat cactttatta
     1441 gcggctgtga ccatcgcacc gctaacggaa taaccctcat taaaggtcgt aataccatta
     1501 accgcatcaa taccatcagc accgacaaac attaaatcgg cattaatatc ctgcaatgaa
     1561 cgctcggcaa tggaaccatg catcgagcgc gttttatggc ggaccgtgcc accacagacg
     1621 accagagtaa tgtctttatt ttcggaaagg gcaaacgctg ccgggagact gttggtaatc
     1681 accgtgatat ttttagcggt cattaatcct tcggcgataa gcattgtggt acttccgctg
     1741 tcgagaataa cggtcatccc ttcatggatc attttgactg ccgcctgcgc tattctgttt
     1801 tttggcgcgc tggcgagctg aaatcgctct ttaaacccga cttcctgggt ctcggtttca
     1861 ctattaccgg acattatttt cgccgcaccg ccatgaaagc gcgtaaccac gcctttttgt
     1921 tcgagaaagc gcaaatcggc acggattgtc gcttccgagg cagcaaatac tcccgccaga
     1981 tcctgaacaa gcacggttcc ctgttcattc actaattgga tgatcttatt ccttcgctcg
     2041 aatgaattca tcgtcattta cccatattca attgtagcta gtgtaaacga agggggacca
     2101 ttggtgaatg atctgttttg cacaataccg ggccagcatc acgctggccc gcggtttcag
     2161 ggaatgagca acactttgcc cggcatggca ttacgagcga tgtcacgcac cgcctgggcg
     2221 aagctttcaa agcttccacg gtgagcgatt aatggctcca ggcttaactt acgttctgtc
     2281 agcaaccggc tcgccgtttc ccactcctgc cccggccaag ggctggagta gttcatccaa
     2341 ctgccgataa ccgtcagctc tttacgcaat attttgccaa acgttgccga tgttaaatgc
     2401 agatcctgat gcaacgtgcc caccagcgcc agttgggcat gaggaccggc aatctctacc
     2461 gccagttcga cagtttgcgg tacgccagcc gtctcgagga taagctgatt aaagcgcagt
     2521 tcgcgtaaaa cgctctgcat ttgcggcgcg ctcatttcgc tactgttaaa tgtttgcatc
     2581 gcaccgaaag attttgccag tgccagtttt tctgagctaa tgtcgatagc cgtcacactc
     2641 tttgctccca gcgcgacagc gcactgaata gccagcaggc caatggttcc ggcaccaata
     2701 ataataacgt ttttattctc acaaccttgc gctaaatgaa aagcatgcag tccaacggta
     2761 atcggctcaa taaaagcccc atcctcaata ggcatatccg tgggtagagc aaagacattt
     2821 tttcgcttaa cgacaatata ttcagcaaat ccaccatcac gccgcgagcc aataaaatca
     2881 tattttgcgc actgggaata aaaccctttc aaacactctg gacaagtaaa acagggtaat
     2941 aacggcacac aggcaaccgc atcgccaggg tgtaaatcat caacaccgga tcccaccgca
     3001 tcaatatagc cgctaaattc atggcctaac gttattggat aataatgtgc accattttta
     3061 aatatcctgg gtaaatcgga accacataag cccgagctgg caatttttac ccgtacctca
     3121 tcctgatgtt taatttcagg aatgacgctt tctgcaacgc gcacgatacc atcagtatca
     3181 ttcaccactg atttcataaa aactcctgat tgttaagggg ggataaactc cccctcaggt
     3241 aaaaattatt ctgcgagaac gactttctct tgtttaataa agccacgcgc tctacgccag
     3301 gtcatgaaaa taccggtcag ataaattgca ccgataataa tgaaaccggg aatattttgc
     3361 ggggagaaaa cctgaatcag taaccaggta atgggagaac cgccctgatc cattgaagcc
     3421 accatacccc cggcttttaa cgccccagca ttagccgcca gttgggtgtg caaaccaata
     3481 gtttgcgtcg cgatccacag ggtgatgctc ataatgatga cacccgagat taaggtgcgg
     3541 aacagatttc cacgatgcac ggcgaccgcc atcgccacga agaagccgat ggtggcaaga
     3601 tcgccaaacg gcagcacctg attacccggc acacaaacag caattaaaat ggtgagtggg
     3661 ataaaaatca ggcttgccga taccaccgcc gtatgtccca gcagcaacgc cggatcaagg
     3721 ccaatcagga actcctgacc gccgaacttc gcctgtaaac gactacgagc ctgcttagcg
     3781 atgggcgtta aaccatccat gatgggttta atcacccgtg gcattagcag catcactgcc
     3841 gccgttttta ccgccagctg caatacacct ttgacatcgt aacccgcgag gatgccgata
     3901 atcagcccca tcacaaaacc cacggtgaca ggctcgccaa atggaccaaa tttgcgctga
     3961 atatcgtcgg cgctaaattt aattcggtta acgcctggga ttttctcgat gatagcatcg
     4021 accagcaccg caatcggccc catatacgcc gacgtaccgt gcggaatagc aataccttcc
     4081 agctcaaaga aatttcgggt atcgcgggca aaccagtcgc cgagcttata aacaaacgcc
     4141 gcgtgaatta ccacacctgc catccctatc atccatgaac cggttgccag gtgcagcaac
     4201 gcgccggtga aggtcatatg ccagatattc cagatatcaa catttaccac ccgcgtcata
     4261 cgggtcagta acatcgccac gttaaccaat atcgcaatcg gaatcgccac cagcgcaatt
     4321 tgcgacgccc aggtcattgg tgaagagccc ggccagccga catcgaccac atgcagattc
     4381 aggtcgaaat tttccgccat cgctttcgcc gccggaccaa tggaatccag cattaagcca
     4441 atcacaaggc caatgccaac aaagccaatc ccgatatgca gacccgcttt aaagcaatcg
     4501 cctgccttca tgcctaatat tttagaaaaa ataatgatga caaccggcag catcactgta
     4561 gggccgaggt cgagaatata acgcatgact tctgaaaaca taggtcaccc ctgtaagata
     4621 gtcagaattt tattttgtaa tgcttcgata ccgacaccag aaataaaagg catgccgtga
     4681 actaacggaa tatcgccaaa actacgatcc actttggcag tggtgcatat caaatgcaca
     4741 ccatccatat aggtttctat ttcattaacc cgacactgga ttaattcaac aggaatatta
     4801 tgattctgac acaactcttt aatttcttcc gccgccatcg tagaggtcgc aaccgcgcct
     4861 ccgcaagcga caataatctt gcgtttcata aatagtcctt tttattgtta cagagggatt
     4921 tattcgtctg aatctaaaac atactttgtg aagtattcct ttaactgggt ttcaggaaga
     4981 gtgattagtg tctcgacgat atcgggctgt tgtaacttgc caaataaaca gcgtaaaagt
     5041 ttcaattgct gctgcggatt ttccacaatc aacgcaataa ccaacgatac cgccacgtcg
     5101 ttatcatcat ccgcttgctg aaaatgaact ttatttgttg gccttaacag ataaatggct
     5161 gacgacttag catgaatcgc ctcacaatgc ggtattgcaa tggcgtgctg ctcaagcatt
     5221 atcccggtag ggaattctgc ttctctggca attaatgcct gtggccaggt atcatgaacc
     5281 acacctttgg cgagcatctc attaccgata tgggttaaaa cttcgctacg atcgacaaaa
     5341 gaaattccgc tacgaacaaa caggttagtc atataccgtc cttattccgc acagccgtag
     5401 cgataggcgc gcaaaacatc ataaatttta tccataatca gctgatgcgg tattgctgat
     5461 aattccccgg actgaatgcg ttcaaattgt ttgggaagat actgactaat catgcccagt
     5521 gggatgtcca cgccttcaag attcaccatc atcgtttcga cgctattttt aatccgacta
     5581 tgcggccagt aataacgaat acgatccgac aggctgtaac gaatatccag taatgaatcg
     5641 ttaaaacccg tacgataata ttttttccag tattgcggtt cgtcgagcat cacttcttca
     5701 attaccgcca ggcaaccgct gcgattttca ggggcgataa gttcctgctc aatttgtgcc
     5761 agtgcaaata tcgcctcgcg taaagcaaag gttaatgcgg gaccgacttt caatattgca
     5821 aagtgatcgc ggactaattc ccaataagcc gtccgggtct ggtaatcggt agaatgtgct
     5881 tcataaacca ttcgggtgtt ttctatccat tgcgccagcg gctgcgcttc ctgcggctga
     5941 taatggataa tattgctgtg atcaaattcc acgcccggct gcaccacgat ggcaatcacg
     6001 cgtgttaacg cctctgtcag cccacgggca ataaaggcct tttgatgcgt acgtaaagta
     6061 ttggcggtat cttcaacatg ggtgatgtgt actgactgaa tggcgctggc ctcaccgccc
     6121 ggaaccggaa cttcggtgcc aatgacatag ctcagttgct cacgctggca atctgtcgcc
     6181 acactttccg cagcaaagca aagcacagca gctcgttccg caaccgtttc tggtgctaac
     6241 ggtatgggat cccccgcgca ggacattgac gcatcaagat gaattttact gaagccggca
     6301 cgaacatatt cctttaccag ctcgacggat ttttccatcg ccgcatccgc attttcttgc
     6361 tgccagcagt ttggccccag atgatcgccg ccgagaataa tgcgttcgcg tgcaaaccca
     6421 actttatcgg caatcgtaaa aacaaattcg cgaaagtctg ccggtgtcat tccggtataa
     6481 ccgccaaatt gattgacctg gtttgacgtt gcttcaatca gcactttgcg cgtgctgttg
     6541 cgatcaaatg ccagcgccgc ttcgataacc aacggatggg cagaacagac tgaacatatg
     6601 ccgatatgtt caccagcttt atgccgggca attaacgttt tcatgttttt tccttattaa
     6661 atggcaggtg cgttatgccc tgccctcgca gccacaatcg gcaatcactt tgctcaccac
     6721 atcgcgcatt gcggatttag ccgactgcaa ataatcccgg ggatcggtcg cttcagggtg
     6781 ctcggtcagg taatttttta acgcctgcga gaaggcattt ttcagctccg ttgcaacgtt
     6841 gattttgcat atccccagtt tgatggtttg ctgaatatcc ttagtcgata accctgacgc
     6901 gccatgcagc accagcggta agttcaccca ctggcgaatg ttctccagtc gagaaaaatc
     6961 aagcgccggt gcgctggcat acatcccatg agccgtgccg atggcgaccg ccagggaatc
     7021 aattccggtt gcctcggcaa attcacgcgc ctgagcgggg ttggtataaa aagcatcggc
     7081 ttcattgact tgcacatcat cttcctggcc gccaagttgc cccagctccg cttcgacgct
     7141 gacatcaaag cgatggcaaa aatccaccac ctctttgacc cgtgaaatat tttgcgcaaa
     7201 aggcaaatgc gaggcgtcaa tcatgactga gcgcacgcca gaacgaacct tctgagcgat
     7261 atcgtcaaat ttcgtgtgat ggtcgagatg aattgccagt ggatggtgat attgcttcgc
     7321 catcgcgctg accagcgcca acagattttc tgtaccagca tgagtaaatg tgccaggcgt
     7381 tccggcgatg atgaccggcg catgcaggtt ggcagcggtt tccaccacca cttgcatcgt
     7441 ttcgagattg tgaatattga atgccggaac cgcataaccg ccgcgctgtg cgttgttcag
     7501 catctgcttt gtcgatacca cgtacatttt catatcctgt cgtttgtttt cgatttcaaa
     7561 atataatgaa atgatttgtt tttaaatgtc gagataacga tcacaaaaac gacaatgtga
     7621 aaattgttcg aggagtaaaa ggcaaaaaaa aacggcctcc cgatagggaa gccgtagcaa
     7681 agtgcgcgtg tttttatgcc ggatgcggtg taaacatacg tggcagtgct atgtaggcat
     7741 gataagacgc ttccgcgtcg catcaggcat aggttgccgg atgcagtgta aacgccttat
     7801 ccgtcctacg gggtggtgct gttcgtatga ggcgtatcag gcaatagtaa ttttgctatc
     7861 gagataaacg tcctgcacgg cattaatcag tttcacgccg tcagccatcg atttcttgaa
     7921 cgccttacgt ccaagaatca gccccattcc gccagcgcgt ttgttgataa ccgcagtacg
     7981 cactgcatca ctgaggtcag tttcaccgcc tgcagcaccg ccggagttaa tcagccctgc
     8041 gcgccccata tagcagttag ctaactgata acgcaccaga tcaatcgggt tttcgctggt
     8101 taacttgctg tacacgcgat cgtcggtata accgtaatta attgctttat agccgccgtt
     8161 attttccgcc attttttgtt tgacgatatc tgcaccgatg gttgccgcca gatggtttgc
     8221 ctgaccggtc aggtcggcgg aaacatggta atcaacgcca tctttcttaa aggcggagtt
     8281 acgcaaatag gcccacagca ctgtcaccat acccagctcg tgcgcacgtt caaaagccgc
     8341 agaaatttct tcaatctggc gacgtgactc ttccgagcca aaatagatag tcgcaccaac
     8401 cgcaaccgcg cccatgttga acgcctgctc cacgctggca tacagcgttt gatcgtaggt
     8461 attcgggtaa cttagcgtct cgttgtgatt aagtttgacg aggaatggaa tgcgatgcgc
     8521 ataacgccgc gataccgacg ccagcacgcc gtaagttgac gccacacagt tacagcccgc
     8581 ttcgatcgcc agttcaacaa tgtttttcgg gtcaaagtag agcgggttag cagcaaatga
     8641 agctccggca gagtgctcaa cgccctggtc aaccggcaga atagaaagat atcctgtgcc
     8701 agccagacgc ccggtgttgt acaacgtctg catattacgt aacaccgctg gcgggcgatt
     8761 attgtcaatc attacgcggt ctacgtagtc atgtccgggg agataaagct ggtcagaagg
     8821 aattgtcata caacggtgct gtaaaaggtt gtcggcgtct ttgccaagca actgcgcaat
     8881 atctgtcatt actatgctcc cgtaaattcc gattggatat cggctatgga ttgtcctggc
     8941 ccgccttttg cgggcaatca taatcctggt cgttacgggc gagtttttcc atcttttgga
     9001 tgtttttcag cgttttctgt tggctcgatt catcagaaaa aatgttatcg cggtcaagtt
     9061 ttcaccgcaa aggtatttaa aaggtattat taaatggtat tgtcatcgcg taccttacat
     9121 tacctgtcat gaaggaatta aaagatgaaa acaacagcaa agctgtcgtt catgatgttt
     9181 gttgaatggt ttatctgggg cgcgtggttt gtgccattgt ggttgtggtt aagtaaaagc
     9241 ggttttagtg ccggagaaat tggctggtcg tatgcctgta ccgccattgc ggcgatcctg
     9301 tcgccaattc tggttggctc catcactgac cgctttttct cggcgcagaa agtgctggcg
     9361 gtattgatgt tcgctggtgc ggcgctgatg tatttcgctg cgcaacagac cacttttgcc
     9421 gggttcttcc cgttactgct ggcctactcg ctaacctata tgccgaccat tgcgctgact
     9481 aacagtatcg cttttgccaa cgtgccggat gtggagcgtg atttcccacg cattcgtgtg
     9541 atgggcacta tcggctggat tgcctccggt ctggcatgtg gatttttgcc gcaaatgctg
     9601 ggttatgccg atatctctcc gactaacatc ccgctgctga ttaccgccgg aagttctgct
     9661 ctgctcggtg tgtttgcgtt tttcctgccc gacacgccgc caaaaagcac cggcaaaatg
     9721 gacattaaag tcatgctcgg cctggatgcg ctgatcctgc tgcgcgataa aaacttcctc
     9781 gtctttttct tctgttcatt tctgtttgcg atgccactgg cgttctatta catctttgcc
     9841 aacggttatc tgaccgaagt tggcatgaaa aacgccactg gctggatgac gctcggccag
     9901 ttctctgaaa tcttctttat gctggcattg ccgtttttta ccaaacgctt tggtatcaaa
     9961 aaggtattat tacttggtct ggtcaccgct gcgatccgct atggcttctt tatttacggt
    10021 agtgcggatg aatatttcac ctacgcgtta ctgttcctcg gcattttgct tcacggcgta
    10081 agttacgatt tttactacgt taccgcttac atctatgtcg ataaaaaagc ccccgtgcat
    10141 atgcgtaccg ccgcgcaggg gctgatcacg ctctgctgcc agggcttcgg cagtttgctc
    10201 ggctatcgtc ttggcggtgt gatgatggaa aagatgttcg cttatcagga accggtaaac
    10261 ggactgactt tcaactgggc cgggatgtgg acttttggcg cggtgatgat tgccattatc
    10321 gccgtgctgt tcatgatttt tttccgcgaa tccgacaacg aaattacggc tatcaaggtc
    10381 gatgatcgcg atattgcgtt gacacaaggg gaagttaaat gaaaacagaa cgtattctcg
    10441 gtgctcttta tgggcaggcg ttaggggatg cgatggggat gccctccgag ctttggccac
    10501 gcagccgcgt taaagcacac tttggctgta ttgaccgttt tctgcctgga ccaaaggaga
    10561 ataacgcggc ctgttatttt aaccgcgccg aattcaccga cgatacctcg atggcgctgt
    10621 gtctggcgga tgcgttactg gaacgtaaag gcaagatcga tccggatctg attgggcgta
    10681 atattctcga ctgggcgctg cgtttcgacg cctttaacaa aaacgtacta ggtccgacct
    10741 cgaagattgc gcttaacgcc attcgcgacg gtaaacccgt tgctgaactg gaaaacaatg
    10801 gcgtgaccaa cggcgcagcg atgcgcgtct cgccattagg ttgtttgctt ccggcgcgcg
    10861 atgttgattc ctttattgat gatgtggcgc tggcgtccag cccgacccat aaatccgatc
    10921 tggcggttgc aggtgcggta gtcatcgcat gggcgatttc ccgtgccatt gacggagaaa
    10981 gctggtcagc gattgttgat tctctaccct cgattgcgcg acatgcacag caaaaacgca
    11041 tcaccacctt cagcgcctca ctggcggcgc ggctggagat tgcgctgaaa attgtgcgca
    11101 atgccgacgg caccgaatcc gccagcgaac agctttacca ggtcgttggc gcaggtacca
    11161 gcactattga gtccgttccg tgcgccattg cgctggttga actggcacaa accgatccga
    11221 accgttgcgc cgtcctctgc gctaaccttg gcggcgatac agacaccatc ggtgctatgg
    11281 cgacggcaat ctgcggcgcg ttgcatggca ttaacgctat cgatcctgcg ttaaaggcgg
    11341 agctggatgc ggtaaatcag cttgatttca accgctatgc cacagcgttg gcgaaatatc
    11401 gtcaacaacg ggaggtggta tgagcggcgc tcgattacac acgctgctgc ctgaattaac
    11461 cacgcgtcag ccggtgatgg tcgtcggcgc ggcggtcatt gacgtgatcg ccgacgctta
    11521 tgccctcccc tggcgtgggt gcgatatcga actgaaacag cagagcgtta acgttggcgg
    11581 ctgcgcactg aatattgccg tggcgttaaa gcgcctcggc atcgaagcgg gtaatgcctt
    11641 gccgctcggt cagggcgtgt gggcggagat tattcgcaac cggatggcaa aagagggctt
    11701 aatcagcctg atcgataacg ccgaaggtga taacggctgg tgtctggcgc tggttgagcc
    11761 ggatggcgaa cgcactttta tgtcattcag cggtgtggaa aatcagtgga atcgccagtg
    11821 gctggcgcga ttaaccgttg cgcctggcag cctgctctat ttttccggtt atcaactggc
    11881 ctcgccctgc ggcgaattgt tagtggaatg gctggaagag ctacaagacg tgacgccgtt
    11941 tatcgatttt ggcccacgta ttggcgatat cccggatgca ttactggcgc ggatcatggc
    12001 ctgtcgacct ttagtgtcgc tcaatcgtca agaggctgag attgccgccg aacgttttgc
    12061 tttatccgca gagataacaa cacttggcga gcaatggcag gagaaatttg ccgcgccgtt
    12121 gatcgttcgt ctcgataaag aaggcgcatg gtatttcagc aacgacgctt ctggctgcat
    12181 tccggcattt ccaacgcaag ttgtagacac cattggggcg ggcgacagtc atgcaggtgg
    12241 cgtacttgcc gggctggcct ctggtctgcc actggcggat gccgtattac tgggcaatgc
    12301 agtggcgtcg tgggttgtcg ggcatcgggg cggtgattgt gcgccaacgc gcgaggaact
    12361 actcctcgca cacaaaaacg tatagatcgc tgcgacagtg gctaatgctg tactcaatag
    12421 gccgttgctg ttggtcaagc gccacttgct tgatcaccag caccggtatt ttgctatcta
    12481 actgaatatg tgactgaaac tcggcatccg gcatccgggc gctaacgcgg gaacgcgtac
    12541 gttgtgggta aatgtgctgg ctgcggaaat agtcataaag tgaaatcccg atggcatcaa
    12601 catcatgaat taaatgcgcc ggaacccacg attcctcaat cgataccgct tcttcatcga
    12661 catagcgaat acgcttaagc aaaaagacat cacttcctgc ttcgaccgcc agctgctgcg
    12721 cgacttcttc gggacatttc acgacacgct tgttaaccca tagcgtatcg ggctttttcc
    12781 cgcgcaatac cacctgctga gaaaaacctc gcgcttcttt caacgagtat tcgaagatgt
    12841 tgttgatctg cgtgccgtaa ccgcgcgaac gcgtcaccac accctcttct tccagcgcct
    12901 gcatcgcctt gcgcaccgta atgcgcgaaa cgccggttaa ctgacttaaa tcacgctcac
    12961 cgggcaaaat attgccatgc tccagcaccc cgctgcgcac ggcatttttt accgtttcgg
    13021 caaacttaat ataaagcggc gtgttatccg ccgctaaaat acgctcgttc agttgggcaa
    13081 tcagctgggt atgcgcttgt tccatttgtc tttttcctgc cgaggagtct ggtgggagta
    13141 tatcactgct tcttatttaa ggcgtttctt taatcccatc aacgaatgcc tgcaactcct
    13201 ccaccgtgcc atgaaataca ttaaaatcca ccggaccatt gatgccatct acctgtccac
    13261 ggtcggaatg ctgccagaag cgccaggcca tgccgtcatt gtccggacga cgttgataat
    13321 agtgagccac ccaccacgga tattcattga aatagcccgc cagattggtg tgataaaaaa
    13381 cggctcctga gtaaataatc ggcttttttc ccgtactttt ttcgaccatt tttagccact
    13441 gacttactcg tttgcgtaat tcttttgccg ataattttcc ccgttcttct acgtccagta
    13501 ccgcagggaa atcgccttgt gagaaatcca ccgtttgcag aaataatcgt gcctgaaccg
    13561 gcgctgctac cgacggggag aaataatgat acgccccgcg cagcaggcca ttttcgcggc
    13621 ttagttgcca gttacgcgaa aaatacgggt ccaccagctt ttcgccttcc gtcgccttaa
    13681 taaaagcaaa ctgtaagcgg ataccgttgt cgcgcatttt tgccacccgc tgccagtcga
    13741 tccgctcctg ccagcgcgaa acatctattc cgtgaatggt ataactggca ggaatgcgaa
    13801 ttgcgaaaga tttaaccggg cgataactgt agaagtggat gtagtctgta atctgaactg
    13861 ctgtcgaata gaaaaaattc accgtctgac gcggataaat tgcaacgatg gaaattagcc
    13921 caagagcgca taatagagag gtgagttttt ttcgactggt aattcttagc tgcattggtt
    13981 tcatccctga atgtcagtgc caaaggctga caataaccaa agcagctata gtacggtgct
    14041 caccaccagg cgtggaagtg gtgaaccgga ccaataccgt gaccaacttc cagcgtgtcg
    14101 gcctgggcta acgccgatga aagccagctt tttgcctcct gtacggtgtc agcccagttt
    14161 gtatggcgcg ggcgtagtgc agccaacgcc gcagagagcg tacaaccagt gccgtgggtg
    14221 ttttgggtca taatgcgcgg tgcagtaaat cgttgttccc cctcacgagt aaacagccag
    14281 tcagggcttt gctcatcatc cagatgacca cctttcatta gcactgcgcc acagcccatc
    14341 gccaacagcg atcgcccttg ttccagcatt tcctgttcgg tgcgcgcgtg tggcgcgtcg
    14401 agcaaggcag cagcttcggg caagtttggc gttattaatg aaacctgtgg caataatcga
    14461 ctgcgcagcg tagcaaccgc cgaaggtgaa agcagcgggt cgccgctttt tgccagcata
    14521 acggtgtcga gtaccacgtt ttggatctga taacgttgca accgttctgc caccgcttca
    14581 acaatatcgg tttccgccaa cataccgatt ttagtggtgt cgattcgcac atcgctgaac
    14641 accgaatcga gctgggcagc gacaaaatca ggctcaatgc gatacaccga ctgtacgcca
    14701 cgggtatttt gcgccaccag tgcagtaatc actgagcaac cataagcgcc aagtgccgag
    14761 aaggttttaa gatcggcctg aatccccgca ccaccactcg gatcagtacc ggcaatcgtc
    14821 agagcgttaa ttcgtttcat gcctgcacct ccggcgtcaa ttgccagagc gcatcaagga
    14881 aatgtggaac aaaactgcct ggcccctcgc tactggcgac tgcgcgttct ccggcttgtt
    14941 tcatccagtg acaggcagat gcgatatttt ccagcgtatc gcctggtagc gcacaacagg
    15001 cagcgacaac cgccgataat gcacagccag ttcctaccac tttggtcatt aacggatcgc
    15061 caccgtgaat accaatgata cgatgtccat cggtaacata atccatctcg ccagtgacca
    15121 cgacgattgc gccagtttcc cgtgccagtg tttgtgcagc gggtatcgca ttagctgcgg
    15181 cgtcagtggt atccactccc cgtccaccat tagcaacgcc agctaatgcc atgatttccg
    15241 aagcattacc acgtatggcg gttggcttat gagacagaag ttccagacaa aaacggcggc
    15301 gataatcgag tgcacccacc gctactggat caagcgtcca gggtgtttga gagcgttttg
    15361 cctgttcaat ggcggcacgc atcgcctgtg cgcgtggctg cgtcagcgtg ccaacgttaa
    15421 tcagcaacgc actggcgata gccgcaaact gactggcctc ttcggtttcg ataaccatcg
    15481 ctggggatgc accgagcgcc agcaaggtat tggcagtaaa ggtttgcacc acatcgttgg
    15541 tcatgcagtg cacaagaggg gaatgttggt gaaaaagatg taacgcgtgc gcagattgcg
    15601 cggaactcag caggtcgact tgcatagttt gctcctgcca taacgtgaag aagcaatgac
    15661 ctggtggtcc gtgacttccc tacgctggca ttatccagat caggtgatac gggtatttct
    15721 cagccttcac gcagaagggc accccgagtc gtttgattgc gatgataaat cgcagaggag
    15781 gatggtaatg tccagcgcac gcgttgtaaa cgagtaaaaa ccgcagtttt acaatcgcgt
    15841 taataaatta tttgatataa gaatccagca ccttcagaac gacatccaga tcttcttcac
    15901 gttttagctc atccccctgg tgaacgatgt gttccgtcag atgaccttta atcacttccc
    15961 gcatcagacc gtttaccgcg ccacggatag cagcaatctg ttgtaaaact gcagcgcatt
    16021 cgtgcggctc gtcgagcatt ttcttgagcg ccacgacctg gccctgaatc ttactggcac
    16081 gcgctttcag tttctgttta tcacggatgg tatgagacat ggcaacacct ggttaataag
    16141 aatatgaaaa atcatagcac tattaatcta ctggggggta gtatcaggta ctggggggga
    16201 gtagaatcag attgccgaat taatactaag aattattatc atgaccgaat ttacaactct
    16261 tcttcagcaa ggaaacgcct ggttcttcat ccccagcgcc atcttacttg gtgcgcttca
    16321 tggcctggaa ccagggcact caaaaacgat gatggcggcg tttatcatcg ccatcaaagg
    16381 caccattaaa caggcggtga tgctcggact ggcagcaacc atttcgcata ccgcagtggt
    16441 ctggttgatt gcctttggcg ggatggtgat cagcaagcgc tttactgctc aatcagcaga
    16501 accgtggctc cagttgattt ccgcagtgat cattattggc accgcgttct ggatgttctg
    16561 gcgtacctgg cgcggcgaac gaaactggct ggagaatatg cacgagcatg atcatgagca
    16621 tcatcaccac gatcacgaag atcaccacga ccatggacat catcaccatc acgaacatgg
    16681 cgagtatcag gatgcccatg cacgagccca tgccaatgac attaaacgac gctttgatgg
    16741 tagagaggtc accaactggc aaattttgct atttggctta accggtggcc ttatcccctg
    16801 cccggcagca attaccgtgc tgttgatatg cattcagttg aaagccctga cactgggcgc
    16861 aacactggtc gtcagtttca gcattggcct ggcgttaacg cttgtcaccg taggcgttgg
    16921 cgcagcaatc agcgttcagc aggtcgcaaa acgctggagc ggatttaaca ctctcgctaa
    16981 acgcgccccc tatttttcca gtctgttgat tggcttagtt ggtgtgtata tgggcgtaca
    17041 tggcttcatg ggcataatgc gataactcaa tcctttaggc ttgcatgtta tcttcagccc
    17101 aggatgattc ttaaatcagc tatttccgct gactctctgc tcgctaagga tgcttttagg
    17161 gcatcctttc atttacactt tttacgaaat catgggatca ctaacaaaat atcgcttgcc
    17221 agttatattg tatggcagga aagatatgcg actgatatta cagatcccca aagtggagag
    17281 tttatgacca ttaaaaataa gatgttgctg ggtgcgcttt tgctggttac cagtgccgcc
    17341 tgggccgcac cagccaccgc gggttcgacc aatacctcgg gaatttctaa gtatgagtta
    17401 agcagtttca ttgctgactt taagcatttc aaaccaggcg ataccgtccc agagatgtac
    17461 cgcaccgatg agtacaacat taagcagtgg cagttacgta acctgcccgc gcctgatgcc
    17521 gggacgcact ggacctatat gggtggcgcg tacgtgttga tcagcgacac cgacggtaaa
    17581 atcattaaag cctacgacgg tgagattttt tatcatcgct aaaaaaagcc ccctcatcat
    17641 gagggggaaa tgcagacacc ttgttatttt ttattattag ccacttgctc gtcttgcttg
    17701 ttattagtcg tatttcacgt tgattaatgc ggttgcctcc agtgcgccag atttaacttt
    17761 gtttgtatcg tagacgtagt aactggcagt tatcggaatt gaaaccgttg tatctgtaag
    17821 ttttgacttc gcagcaactt cctttaaatt tgtatatcct ttactgctgt tagtgatatt
    17881 taaaagtgtg actggcgaac cattactttc aagaataaac cctacgccat tatcttccgc
    17941 acctttcagg atcgtgttac tactgcccca caaatcagaa cttgataaat acacctgcat
    18001 ttttcgtgat gtttgatttt gttgacctaa ta
//
LOCUS       DACBVI010000091        18012 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.091, whole genome shotgun sequence.
ACCESSION   DACBVI010000091 DACBVI010000000
VERSION     DACBVI010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.091"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(152..1744)
                     /gene="lsrK"
                     /locus_tag="IU325_14910"
     CDS             complement(152..1744)
                     /gene="lsrK"
                     /locus_tag="IU325_14910"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HAP5810297.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNND
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1823..2776)
                     /gene="lsrR"
                     /locus_tag="IU325_14915"
     CDS             complement(1823..2776)
                     /gene="lsrR"
                     /locus_tag="IU325_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HAP5810298.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIASAMKGGYINALVTDQDTAAAILRS"
     gene            3025..4560
                     /gene="lsrA"
                     /locus_tag="IU325_14920"
     CDS             3025..4560
                     /gene="lsrA"
                     /locus_tag="IU325_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HAP5810299.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYTRLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTIELSTDDIIQAITPALREKSLSASQKLWLELPGNLAQHAAGTSMLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4554..5582
                     /gene="lsrC"
                     /locus_tag="IU325_14925"
     CDS             4554..5582
                     /gene="lsrC"
                     /locus_tag="IU325_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HAP5810300.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILMAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGMVLLAVLVFDGRLRCALERNLRRQKYARFMMPPPSVKPAS
                     SGKKREAA"
     gene            5582..6574
                     /gene="lsrD"
                     /locus_tag="IU325_14930"
     CDS             5582..6574
                     /gene="lsrD"
                     /locus_tag="IU325_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HAP5810301.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6586..7608
                     /gene="lsrB"
                     /locus_tag="IU325_14935"
     CDS             6586..7608
                     /gene="lsrB"
                     /locus_tag="IU325_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HAP5810302.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADELLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7635..8510
                     /gene="lsrF"
                     /locus_tag="IU325_14940"
     CDS             7635..8510
                     /gene="lsrF"
                     /locus_tag="IU325_14940"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HAP5810303.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVDKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8534..8824
                     /gene="lsrG"
                     /locus_tag="IU325_14945"
     CDS             8534..8824
                     /gene="lsrG"
                     /locus_tag="IU325_14945"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HAP5810304.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFFIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8881..9639
                     /locus_tag="IU325_14950"
     CDS             8881..9639
                     /locus_tag="IU325_14950"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HAP5810305.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIKYVADLGCGPGN
                     STALLHQRWPAARITGVDSSPAMIAEARSALPDCLFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLSPLGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     KRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9643..10557)
                     /locus_tag="IU325_14955"
     CDS             complement(9643..10557)
                     /locus_tag="IU325_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810306.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10764..12215)
                     /locus_tag="IU325_14960"
     CDS             complement(10764..12215)
                     /locus_tag="IU325_14960"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP5810307.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12442..13389)
                     /locus_tag="IU325_14965"
     CDS             complement(12442..13389)
                     /locus_tag="IU325_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAP5810308.1"
                     /translation="MLADWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALI
                     VSVIASVVIGGAGSLAFPLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISV
                     GSPFSIPEMFSARLGIATMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGL
                     YEALKSPSLKQTQHLTVMLLDIDYFKSINDNYGHKCGDKVLSVFARHIQKIVGDKGLV
                     ARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASY
                     RTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(13611..13860)
                     /locus_tag="IU325_14970"
                     /pseudo
     CDS             complement(13611..13860)
                     /locus_tag="IU325_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005153809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(13999..14358)
                     /locus_tag="IU325_14975"
     CDS             complement(13999..14358)
                     /locus_tag="IU325_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HAP5810309.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLDKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRCCLAKWHNIPQGVSLSEQQQR
                     YIVAVIYHWLVIQMNQP"
     gene            complement(14358..15284)
                     /gene="glsB"
                     /locus_tag="IU325_14980"
     CDS             complement(14358..15284)
                     /gene="glsB"
                     /locus_tag="IU325_14980"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HAP5810310.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15348..16736)
                     /locus_tag="IU325_14985"
     CDS             complement(15348..16736)
                     /locus_tag="IU325_14985"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HAP5810311.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITSEMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAITVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGAHLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETRARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16837..17718
                     /locus_tag="IU325_14990"
     CDS             16837..17718
                     /locus_tag="IU325_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP5810312.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTLPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            17796..>18012
                     /gene="yneK"
                     /locus_tag="IU325_14995"
     CDS             17796..>18012
                     /gene="yneK"
                     /locus_tag="IU325_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HAP5810313.1"
                     /translation="MVTPVSISNYISLPDNFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTFLNINSATKKIFNNLDNLSYIQRT"
BASE COUNT         4507 a         4602 c         4489 g         4414 t
ORIGIN      
        1 aatagcaatt tccccttgaa tatattactg gcgatggaac gatgaatctg caagctggca
       61 tttaacaaac cagctctaaa agaagggaaa tgagaatacc aatactcaaa aactaacagc
      121 cacggtcatc atgatgtggc tgtcaatgaa actataaccc aggcgctttc cataacgacg
      181 tcgtcagccc gtgatcaacc agccccagct gatcctgata aactgcctgc cacttatcgc
      241 gtgaatcctg ataaagttca tgcttttccg ggtctggtgt gtgcgtccgt tcccagcgaa
      301 ccaggcgttc tccggtttct gccattgatg aaaaaattcc ggcaccgacg ccagctgcaa
      361 tggcacatcc taatgcagtg gcttctttga ccaccggaat attgacgggt aatcccgaga
      421 catcagcgag aatttgactc cataatttcc cttttgaacc tccgcctgca aagactaacg
      481 atgaaggatg aatattcgag aaatcagcga tttgctgcaa gttacacgct gatactatcg
      541 ccgcattttc ttccagcgca cggaacaatg tcgctttgtt acatttatcc ggatcaatag
      601 acaaattaat aaaggaaggc gcagcgtgat accaggtttt aaagcgcatt ctgtcggaga
      661 atatcggcat tacgccccac gacccaggcg gcacccgact tgccatctct tcaagcagcg
      721 tataggtgtc gatgcctaaa cgttcggcaa tcagtttttc ttcggcacag aaagcatcgc
      781 ggaaccagcg catggtgagt ccggtaaaaa agcttataga ttcagcttgt accatgccag
      841 gaataacatg aggattaacg cgcacgttca tttctgggtc tgtcaccggc gcggctaagt
      901 ttacaacttg ctgccagaat gtgccgccaa gaacagcggt ttgtgccgga cggacaacgc
      961 ctaacccaag gcaaccaagt tgcacgtcgc ctcctccaac gaccaccgga gtgcccgcct
     1021 tcagaccgca gagttccgcc gcttgtgaac ttaccacgcc cagcaatgtg ccggtttctt
     1081 tgacaggaga aagaatatcg gcccgcagtc cggccatatc cagcaatgca ggtttccagt
     1141 cacgggtggt tagatcaaga agtcccgtgg tgccagcgtt agagggatcc accgccagtt
     1201 cgccgctgag catataggcc agccagtcgc tgatcatggt gatggtcgac gcctgtcggt
     1261 agatatcaga acgatggtgc gccagccaaa gtaatctggg aatagcgctt aaagccagcg
     1321 tttgtccggt ggcgcgataa acttcgtttt caaaggtatt gttgtgcagt tctttaagtt
     1381 cgctaacttc gcgcgccgct ctggcatcca cattggcgca ggcccagatc ggtgttccat
     1441 cattgttata taaaacaatg ccttcacgca tcgaacatgc cgaaacggca gcgatatact
     1501 ccggggctat gccggcgttg tgcagcgcct ggcgcataca ctcacacgcc agttgccagt
     1561 ttttgttgag atcaaattcc atagaaccag gaacgtccgg tactgccagg tgccgccact
     1621 ccgcctgtcc cacggctatt tgattgcctt ccaggtcgaa tatcacagcc cgaatacttc
     1681 cggtacctgc atccagcgcc atcaggtaat actttgattc tgaaggggta aagagtcgag
     1741 ccattagatt atcctcggct tatgcgttct gtctgttcct ctgtacgttc tccatcattc
     1801 ccggtaataa ggtcatgcaa atttaactac gtaaaatcgc cgctgctgtg tcctgatcgg
     1861 taaccagtgc gttgatataa ccgcctttca ttgcagaggc aatggcttcg gctttatttt
     1921 ctccccctgc tacgccaacc cggacgggaa tggtcttcag cgcgcttaaa ggtaagccaa
     1981 tcagttcgtt atgtattttg atatccgtga caacgtcacc ttttgcatca aaaaagtagc
     2041 ctaagatgtc gccaaccgcc ccttttcggc caatcattaa ctgttcgccc tggctgatat
     2101 aaccggagcg aatgatcgtc gcgtcgtctt gttgactcac ggccccaatg ccgacaatcg
     2161 ccacatccgc tgcttgcgcg gctaacagaa catctttgac gcaattttca ttttttagcg
     2221 tacgggcaat gtcagcggag gatgcccgca gcggagccgg aataatattc acactgcacg
     2281 ccgcgttaag ctgtccgata cccgtcatat aagaaccgac gccaccggag agcgtgacca
     2341 ggcgaatttg ctgcgacgaa ataaaaccac ttaagcgttg cagcgtattc atggttgcct
     2401 cgccaaaacc aatcgccagc atctgttgtg gttgaagtaa actcatcaac atatgcgccg
     2461 cgcctatccc tagtcgcccg ccgacatcag catccgcaag ccctgggatc acccggacat
     2521 gttgcagcga aaactgacga cgtaattgtg tttcatattc cagacagcct tcaaagcgag
     2581 aattaatctg tacgcgaata atgccggatt gatgcccttt ctccagtaat cgcgacactt
     2641 tcaaacgggt caggccgaga cgatcgctga tctcactctg ggtcagcccg tcgtggtagt
     2701 aaaaccacgc aatccgcgcg acctgttctt cttcacacat tccctgttct gaaattaccg
     2761 aatcgttgat tgtcataatt cattcttcac tttgaacata cttaaatctt taatgcaatt
     2821 gttcagttat tgcgcattta tatctgtgat ggcaaccgca gtttgactct acgagcatga
     2881 acaaacgcaa ccgtgaaaat caaaatagca taaattgtga tctattcgtc ggaaatatgt
     2941 gcaatgtcca cctaaggtta tgaacaaatt aaaagcagaa atacatttgt tcaaaactca
     3001 cctgcaaaac tgaacggggg aaatatgcaa acgagtgata cccgcgcgtt accgctactt
     3061 tgcgcccgct cggtttataa acagtattca ggggttaatg tcctgaaagg catcgatttt
     3121 acgttgcatc agggggaggt ccacgccctg ctcggcggca atggtgccgg taaatcgacg
     3181 ttaatgaaga ttattgccgg tattacccct gctgatagcg gtacgcttga gattgggggc
     3241 aacaactaca ccagattaac gccagttcat gctcatcagt tgggtattta tctcgttccc
     3301 caggaaccgc tgcttttccc aagcctgtcg ataaaagaaa acatcctgtt tgggctggca
     3361 aaaaaacagc tctccatgca gaaaatgaag aacttgctgg cggcgctggg ctgccagttt
     3421 gatctgcata gtctggcagg atcgctggat gtcgccgatc gccaaatggt ggaaatcctc
     3481 cgcgggctga tgcgcgactc gcggattctg atcctcgatg aacctaccgc ctcgcttacc
     3541 cctgcggaaa ccgaacgctt gtttactcgc ttgcaagagc tgcttgctac tggcgtgggt
     3601 attgttttta tctcgcataa gctgccggaa attcgccaga ttgccgatcg gattagcgtg
     3661 atgcgcgacg gaactatcgc cttaagcggc aaaaccatcg aactgtctac cgacgacatt
     3721 attcaggcca tcacgccagc gctacgggaa aaatcgctct ctgccagcca aaaattatgg
     3781 ctggagttac ctggcaacct cgcacaacat gccgccggaa cgtcgatgct gacactagaa
     3841 aatctgaccg gcgaaggttt caggaatgtc agcctgacgc tcaatgccgg agaaattctg
     3901 gggctggctg ggctggtggg ggccggacgc acagaactgg ccgagacgct ctatggtctg
     3961 cgtactttgc gtggcggacg cattatgctg aatggtaaag agatcaataa attatccact
     4021 ggagaacgtt tactgcgcgg tctggtttat ctgccggaag atcgccagtc atccggactg
     4081 aatctcgatg cttcgctggc ctggaacgtc tgcgccctta ctcataacct tcgtggattc
     4141 tgggcgaaaa ccgcgaaaga taatgccacc ctggaacgtt atcgtcgggc gctgaatatt
     4201 aaatttaacc aaccggaaca agctgcacgc acattatccg gtggcaacca gcaaaaaatc
     4261 ctcattgcca aatgcctgga agcttcgccg caagcattga ttgtcgatga gccgacgcgc
     4321 ggcgtggatg tctcggcccg taatgatatc taccagctgt tgcgcagcat cgccgcacaa
     4381 aatgtggctg tgctgcttat ctcctcagac ctggaagaga tcgaactgat ggcagatcgt
     4441 gtgtatgtga tgcatcaggg cgaaattacc cactctgcac tgaccgggcg cgatattaat
     4501 gtcgagacta ttatgcgcgt tgccttcggc gatagtcagc gtcaggaggc atcatgctga
     4561 agtttattca gaacaaccgt gaaatcacgg cattgctggc ggtggtgctg ctgtttgtat
     4621 tacccggttt tctcgaccgc cagtatttaa gtgtgcaaac gctgaccatg gtttatagca
     4681 gcgcgcaaat cctgatcctg ctggcaatgg gcgccacgct ggtaatgctt acgcgcaata
     4741 ttgatgtttc agtgggttcg attaccggaa tgtgcgcggt gctgttgggg atgttactga
     4801 acgcggggta ttcactacct gttgcttgtg tcgcgacttt actgcttggt ttgctcgcgg
     4861 gatttttcaa cggtgtcctg gtcgcgtggc taaagatccc tgccattgtc gccacccttg
     4921 gcacgttagg gttgtacaga ggcatcatgt tgctgtggac tggcggtaaa tggattgagg
     4981 gattacccgc cgaactgaaa caactctccg ccccgctgct gtttggcgtt tccgcgattg
     5041 gttggttgac gataattctg atggcattta tggcctggct gctggcgaag acggcgtttg
     5101 gacgcagttt ttatgccacg ggcgataatt tacagggcgc tcgtcaactg ggcgttcgta
     5161 cggaagccat tcgcattgtg gcattttcgt tgaacggctg catggcggca ctggcgggaa
     5221 ttgtgtttgc ttcgcagatt ggttttatcc ccaaccagac cggtaccggg ctggagatga
     5281 aagcaattgc agcctgcgtg ctgggcggca ttagtttgct cggcggttcc ggcgcgatca
     5341 tcggtgcggt actcggcgca tggttcctga cgcagatcga tagtgtactg gtgctgttgc
     5401 gcattccggc atggtggaat gattttatcg cgggcatggt tctgctggcg gtgctggtgt
     5461 ttgatggacg cctgcgttgt gcgctggaac gtaatctgcg gcggcaaaaa tatgcccgct
     5521 ttatgatgcc accgccatcc gttaaacccg cttcgtcagg taaaaaacgg gaggccgcgt
     5581 aatgcgtatt cgctacggtt gggaactggc tcttgccgca ctgctcgtta ttgagattgt
     5641 tgcatttggt gcaattaacc cgcggatgtt agatctcaat atgttgctgt tcagcaccag
     5701 tgactttatt tgcattggca ttgtcgccct accgctgacg atggtgattg tcagtggcgg
     5761 gatcgatatt tcgtttggtt cgactatcgg cctctgcgcc attgcattgg gcgtactgtt
     5821 tcaaagtggt gtgccgatgc cgctggcgat tcttctgacc ttactgctcg gcgcattgtg
     5881 cgggttgatc aacgccggat taattatcta taccaaagtt aacccgctgg tgattacgct
     5941 tggcacgctg tatctgtttg ccggaagcgc cctgctgctt tccgggatgg ccggagcgac
     6001 ggggtacgaa ggtattggtg gattcccgat ggcgtttaca gatttcgcta acctggatgt
     6061 gctgggactc cctgttccgc tgattatctt cctgatatgt ctcctcgttt tctggctctg
     6121 gctgcataaa acccatgccg gacgtaacgt gtttttgatt gggcaaagcc cgcgcgtggc
     6181 gctttatagc gcgattccag ttaaccgtac cttatgtgcg ctctatgcca tgacggggct
     6241 ggcgtctgcg gtcgccgctg tgctgctggt gtcgtatttt ggttcagcac gttccgatct
     6301 cggtgcgtcg tttctgatgc ccgccatcac cgccgtggtg cttggcggtg ccaatattta
     6361 tggtggttcc ggttccatta tcggcaccgc cattgcggtt ttactagtgg gatatttgca
     6421 acaaggtttg caaatggcag gagtgccaaa tcaggtgtcc agcgcccttt ccggtgcgct
     6481 acttatcgtc gttgtcgtag gtcgttccgt tagcctgcat cgccagcaaa ttaaagagtg
     6541 gctggcgcgt cgggccaata acccattgcc ataaaggata tcttcatgac acttcatcgc
     6601 tttaagaaaa tcgccttact tagcgcgctt ggcattgccg caatctctat gaatgtgcag
     6661 gccgcagagc gtattgcatt tattcccaaa ctggttggcg tgggattttt taccagcggt
     6721 ggcaacggcg cacaacaagc gggtaaagag ctgggcgttg atgtgaccta cgacgggccg
     6781 acagaaccca gtgtttctgg tcaggtacag ttgattaata actttgtcaa tcaaggttat
     6841 aacgccatta tcgtttctgc ggtttcgcct gatggcttgt gtccggcact gaaacgcgcc
     6901 atgcaacgtg gtgtgagagt gctgacctgg gactctgata ctaaaccgga gtgccgctct
     6961 tactacatta atcagggaac gcccgcccag ttgggaggta tgttggtgga tatggcggcg
     7021 cgtcaggtga ataaagacaa agccaaagtc gcgtttttct actcaagccc caccgttacg
     7081 gaccaaaacc agtgggtgaa agaagcgaaa gctaaaatcg ccaaagagca tcctggctgg
     7141 gaaattgtca ctacgcagtt tggctataac gatgccacta aatcattaca aaccgcagaa
     7201 ggaatattaa aagcgtatag cgatctcgac gccattatcg cccccgacgc caacgccctg
     7261 cccgctgccg cacaagccgc agaaaacctg aaaaatgaca aagttgcgat tgtcggattc
     7321 agtacgccaa acgtgatgcg tccgtatgtg gaacgcggca cggtgaaaga atttggcctg
     7381 tgggatgtgg ttcagcaagg caaaatttca gtttatgtcg cggatgaatt attgaaaaaa
     7441 ggatcaatga aaacgggcga caagctggat attcagggcg taggtcaggt ggaagtctcg
     7501 ccaaacagcg ttcagggcta tgactacgaa gcggatggta atggcatcgt actgttaccg
     7561 gagcgcgtga tattcaacaa agagaatatc ggcaaatacg atttctgatg tgcattactt
     7621 aaccggagta cgttatggca gatttagacg atattaaaga tggtaaagat tttcgtaccg
     7681 atcaaccgca acaaaatatc ccttttaccc tgaaaggttg cggtgcactg gattggggaa
     7741 tgcagtcacg cttatcgcgg atatttaatc cgaaaacggg taaaaccgtg atgctggcct
     7801 ttgaccatgg ttattttcag gggccgacta ccggacttga acgcattgat ataaatatcg
     7861 ccccgctgtt tgaacatgcc gatgtattaa tgtgtacgcg cggcattttg cgcagcgtag
     7921 ttcccccggc gaccaataag ccggtggtac tgcgggcgtc aggtgcaaac tctattctgg
     7981 cggaattaag taatgaagcc gtggcgttat cgatggatga cgccgtgcgc ctgaacagtt
     8041 gcgcggtggc ggcgcaggtt tatatcggta gcgaatatga acaccagtcg atcaaaaata
     8101 ttattcaact ggtcgatgcc ggaatgaaag tgggaatgcc caccatggcc gtgacaggcg
     8161 tgggcaaaga tatggtgcgc gatcagcgtt atttctcgct ggctacccga atcgccgctg
     8221 aaatgggggc gcagattatc aaaacttatt atgtcgataa aggttttgaa cggattgttg
     8281 ccggatgtcc ggtacccatt gttattgctg gcggtaaaaa gttaccggag cgcgaagcgc
     8341 ttgaaatgtg ctggcaggct atcgatcagg gcgcttctgg tgtggatatg gggcgtaata
     8401 ttttccagtc tgaccatccg gtggcgatga tgaaagccgt acaggcggtg gtacaccata
     8461 acgaaacggc tgatcgggca tatgaactct atctgagtga aaaacagtaa ctgcggattt
     8521 aaggagaaga attatgcacg tcacactggt tgaaattaac gttcatgaag acaaggttga
     8581 cgagtttatc gaagtttttc gccagaacca cctgggctcg gtacaggaag aaggcaattt
     8641 gcgcttcgat gtcttacagg acccggaagt gaattcgcgc ttttttatct acgaagccta
     8701 taaagatgaa gacgcagtgg cgttccataa aaccacgccc cactacaaaa cctgtgtcgc
     8761 gaaactggaa tctttaatga ccgggccgcg taaaaaacgt ctgttcaatg ggttgatgcc
     8821 gtgaggcgaa tttatcaatt ttatctacaa ttggggtaac gcgctgacgg gagtaaaaaa
     8881 atgtctgact ggaacccctc tttatatctg cactttgccg ctgaacgatc gcgtccggcg
     8941 gtggagctgc ttgccagagt gccgctggag aatatcaaat acgttgccga tcttggctgt
     9001 ggcccaggta acagcaccgc cctgctgcat caacgttggc ctgcggccag gataacaggc
     9061 gtcgactcat ctccggcgat gattgctgaa gcgcgcagtg ctttgccaga ctgcctgttt
     9121 gtggaagcag atatccgcaa ctggcaaccg gaacaggcac tcgatctgat ttttgctaat
     9181 gcctcactgc aatggctgcc cgaccactac gaattgtttc ctcatttggt ttcgttactc
     9241 agtccgcttg gcgtgctggc agtacagatg ccagataact ggctggagcc gacccatgtg
     9301 ctcatgcgcg aagttgcctg ggaacaaaac tacccggatc gcgggcgtga gccgttggct
     9361 ggcgttcatg cttactacga tattttgagc gaagccggat gtgaggtcga tatctggcga
     9421 accacctact atcaccagat gccgtcacac caggcgatta tcgattgggt gaccgccact
     9481 ggattacgtc cgtggttaca ggatctgacc gagagcgaac agcagctttt tcttaagcgc
     9541 taccatcaga tgctggaaga gcagtatcca ctgcaagaga acggacagat actgctggca
     9601 tttccgcgtc tttttattgt tgcccgccgt acggagtaaa tatcacgtca gctggtaatg
     9661 acgatcggga agaatttttg ctggaatttc ggcttcatcg ttcatctgta acaggtcaat
     9721 ttcaatagcg ttgcagatgg catccagcgg taaatcattg ttttcagtac cgaacggatc
     9781 ttccagttct tccgccagac aatccagcga aataaaagtg taggaaatca gcacagagat
     9841 aaaaggcgtc atgtaatgca ggtccacgac cagcgcgaac ggcagcatga tacaaaacag
     9901 ataaacggta cgatgcaaaa tcagggtgta ggcaaaggga attggcgtat aggcaatgcg
     9961 ctcgcatcct gccaggaccg ctgaaatatc attaagccga tcgttgaggc taataaacag
    10021 gatatctgaa agctgcccat tgcggcgctg aaccgccaac cactccccca ttattaacaa
    10081 gatacggtta gctggagagt tcgaagccag tacaggctga agatcttcag tcttgagata
    10141 atgagccagc acttccgcct gtggctgttt gcgtaatgtc atgcgtaaac agtgggcgaa
    10201 ggcgatttgc aaccgggcaa actcccttac acttgccgaa tccggcaatg tcgtttttac
    10261 ctcgcgcagt aacgaccgtg aggcaatcat caactgcccc caaagttttc gcgcttcaac
    10321 gtaacgggcg tacccggcat tattacgaaa accaagaaaa atggcgatgg cgacaccgag
    10381 aatgctgaac ggtgcgaggg tgaatttgat gcccagatgc gtgtaccagg gcagcatgaa
    10441 aataacagcg atagaaaaga gaaaattgag tagtaagcgc gatgatatct tggataatac
    10501 tgagccgtgc cagacaaaaa tacggcgcag ccagtgttgt tgtggacgaa caatcatggt
    10561 tatcttcagg cgtgaaaaag tcgccctatt aaacgtgatt acgatcacat tctcaagacg
    10621 ctatttacaa attacctaca agcttacaac attaacaggc gctgcatgtg gcatctgtta
    10681 atgataaagg cacataggat gttgtaacta atatagtgaa ataagaaacc cggtcgaaac
    10741 cgggttcaga agtaacggtg ttattagcac aacggacgta cagcttcgcg catccctttt
    10801 tcaagaatcg catccaggtc attagcaacc tgctcgacca gaccaggcac ttgcgtcagg
    10861 tcctgctccc agtggtcttt ttctgccaac acaatcgcta ccagttcctg ggtaccgata
    10921 acacggtcac gatgctggct ccagagttgc tgataacgtt ccagccagtg tgcatcgtcc
    10981 tgtaccggat atgtttctcc gttacgctca ccacgataga acgcaatcaa tgccgctaat
    11041 gcgaaagtaa ggcgcgccgg aagtgtgccg tttgccttct gccctgccag cagctgcggc
    11101 aggatgcggg tgcggaactt ggtcataccg ttgagcgcga tagacagcag ctgatgctta
    11161 atgtacgggt tacggaaacg cccggtgact gcactggcga aagattccag ttcatcacga
    11221 ggcaaatcca gtaccggaat aatttcttcg tagatggctt tttcaacgaa tgcgcaaatt
    11281 tcagcatcgt tcatcgcttc acctacggta tccagccctg cctggaacgc caccggcacc
    11341 agcgcggtgt gcgcaccgtt gaggattgcc actttgcgtt ctttatacgg tttaatatcg
    11401 tcaacaatca gcacgttgag cggatatttg tccaggcgca gctcagtcgc taaggatttc
    11461 ggcccctgaa tcacaaacag gtaaaagtgt tcagcggtgt cgaggaaacc atcgtgataa
    11521 cccagttctt cttccagttt agccacttca tcgcgcggat aaccggtaac gatacggtcc
    11581 accagcgtag aacagaagct gttagcctga tccagccatt gaataaatgc ttctggcagt
    11641 gcccactctt gcgcatagcg cagcaccagt tcacgcaacg cgtcgccatt gtagtcaatc
    11701 aactcacacg gaatgatgat ccaaccttta tccagcgcac cgttgaaatg gctaaagcgt
    11761 tcgaacagca gacgggtcag ttttgccgga tagcttaccg ctggtgcgtc atcgaattta
    11821 tcgcccgcat ggtagctgat ccccgcttcg gtggtgttag agaaaacaaa gcgcatttcc
    11881 gggttgtgcg ccagtttcag gaattcatcg tattcactgt agacgctgat ttcacgatta
    11941 accgagcgaa tcagacgcgc gtcgctgacc gcttccccct tctcattcag gccacggata
    12001 atggtggtgt acagaccatc ctgcgtgctc agtgacggcg ggaatgaagt ttcaatcgga
    12061 cgaacaacga ccacgccaga attcagatcg gtgtgctcat tcaggagatc gatttgccag
    12121 tcaacaaagg cgcgcaggaa gttaccttca ccaaactgaa tgatacgttc tggatactgt
    12181 gcaccgggaa aatcgcgacg atttagtgtt ttcacaatgg gttcccttct gattagtcat
    12241 acaacctgtt tgaattggta cgacaggtta gcaaacttta atacgccgaa cccctgtttt
    12301 gatcaactcc tgatgattaa tgagcagttt tatgagaaaa gtgtggcgcg gatcatggtt
    12361 taatcgagga aatacgcctt ttcctggatc ataaagtggt agaacacatt gcattcaaat
    12421 cgcgcggaat gaataaagat gtcagacaac ttcctcaccg taacgcatag tgctggtacg
    12481 gttgcgccca tctttcttcg agcgatacag acacgtatcg gcttcgacca tcaatttatt
    12541 aaaatcatcg gtcagcgttc ggtacgatgc gcgaccacta ccgacgccaa tacttaccgt
    12601 gagataaagc gttttttgtt gccaggtgaa tggttgcagt tcaacgcctt tacggatttt
    12661 ttccgccatt agcagaccat cgacaggatt caccgacggc actgcaacag caaattcttc
    12721 gccgcccatg cgcgccacca gccccttatc accgacaatc ttctgaatat gccgggcaaa
    12781 cacgcttaac actttatcgc cacatttatg tccatagtta tcgttaatac ttttgaaata
    12841 gtcgatatca agcagcatga cagtcagatg ttgtgtctgt ttcagcgatg gacttttcag
    12901 cgcctcataa agaccggacc gtgagtaaac ctgagtcaga aaatcaaagt cggctcgcag
    12961 cgcaacttgc ttcattagcg aattgatcgc tgccacgcta aaagaaacca taattgggca
    13021 tatcgccatc gtggcaatac cgagacgtgc ggagaacatt tccggaatgg agaacggcga
    13081 accgaccgag atatcaatca ccgaattagc caccagtacg atttccactg caccggtaac
    13141 aaaggtcaac agacatgtta cctgcggcgt atagcgcact gcacaccaga ttaatgcagg
    13201 cagcgggaac gccagactcc ccgctccgcc aatgactacc gaggcaataa ccgacacaat
    13261 aagcgcgatg gcgggcatca tctgttctgc tttaaagcgc ggcagcactc caggaattgc
    13321 caacgtcagc atacaaggca cgatcaacac gcccgttgag aattgctcac tgaaccagtc
    13381 cgcaagcaaa ggccagaaat ccagactatc aatactgacc gaaccaatcg ccccgacaat
    13441 agcgcataat aatgcggcaa tcagacagta attaaatagc cgtaaggcac ttactggctc
    13501 atacttattt ttccccagac gcttatcccg cacgaccagt aaggcgacgg taacaataaa
    13561 catcatattg gagaaattga tagccagtga aaccagtccc cattcggtgg ttatggcatc
    13621 ataaaccagc atcgccacat aactgatcgc atagtaatgc agtcgattaa gccagacata
    13681 gcgggcaaac acccccgcca ttacgccatt cagcggccag aaaagagaaa gttcgtgaac
    13741 cagccgtaac tctgcaccaa taaaatagaa cagcgtggtt agcgcaaaaa tagcgatgct
    13801 attacgtagc agatgacctt cctggaacaa acggaaagtt gaaatggggt gtacatgcat
    13861 taattatctg tccatacaat tgcgggtggc aattatctca acgtaataag taaattctca
    13921 taaaggatag catgttcgct gacagcggcg gcaaataaag gtaccctagg aatgctattt
    13981 tatcgttttc cgcaattatc atggctgatt catctgtata accagccagt gatagattac
    14041 cgccacgata taacgttgtt gttgctcgct taacgaaaca ccctgcggaa tgttatgcca
    14101 cttagccaga caacagcgac aacaggttgc agtggcgtgc tgagcgataa acaccggatg
    14161 cccgcgcatg ggggtttgct taccgtcatt agccggtaat gccggagcaa gacgtttagc
    14221 gacaaaatcc gccgcatgtt gttcgatgac tggcgcacct ttatccagac aatactgacg
    14281 ctctttcatg cccaaacgaa agcgagatcg aaattttgaa cgggataaac gcgcgaagag
    14341 tggatcgaga gactgcatta ataaaccgaa cgccctaact gtttcgtcaa ttgttcaagg
    14401 acggcaatgc ccgcaagcga gttaccagca tcatccagtt ccggactcca gactgcgatg
    14461 gccatttcat gcggaacaat cgccacaata ccgccaccaa cgccagattt cgccggtagt
    14521 cccacccgcc aggcaaactc ccccgcgttc tggtacatac cactggtcgc catcagcgcg
    14581 ttaatttgcc gtgcctgcat tggcgtcacc acaggttcat caatatgaat agctttcccc
    14641 tgattagcca gaaagacaaa cgtccgggcc agctctacac agctcatttt cagagcgcag
    14701 taatgaaagt agttttgcag aacggttgtc acgtcatgat ggaaattgcc aaacgacttc
    14761 atcagccagg cgatagccgc atttcgcgcg gaatgttcaa attcggaacg cgctaccacc
    14821 gtatcgtagg aaatatcaga cacaccgctt aagccgcgca cgacttccag catacgttgc
    14881 cgtggtgcgc ttaatcgccc ttgcaacata tcgcagacca ccagcgcacc ggcattaata
    14941 aacggattgc gcggaatacc ctgctccatt tccagttgca ctaaagaatt gaacggtgat
    15001 ccagacggat ctttgccgac gcgttgccag atttcctctt cggagtaatg acgcatggcg
    15061 acaacgagac tcagcacttt ggaaatagac tgaatggaaa aacgttcttg cgcgtctccg
    15121 gcctgaaaaa gctgtccgtc aacggtacag atagcaatgc ccaatcggga accgtctacc
    15181 gtagccagcg ccggaatata atccgcgact ttaccctgac caatgagcgg ccgcacttgc
    15241 cgcaagatgt tttctaaaat tgcattatcc atggcgactg ccactttcta ctcctggacc
    15301 gcaggtctga aaagacctgc gagtatatca gagctaaata tgtcgcgtca gatccggtct
    15361 ttccacaccg tctggatatt acagaattcg tgtaagccga aatgggaaag ctcacgacca
    15421 aagccactct ttttcacgcc accaaaggcc actcgcgcgt cgctggcaca ataaccattg
    15481 ataaacaccc caccgcattc cagacgtgcc gccatctgtc tggcccgtgt ttcgtcggtg
    15541 gtaaaaatgg tcgctgaaag gccgaactca ctatcattag ccagttccag tgcatgttct
    15601 gcatctttcg caatggtgat tgccgcaacg gggccaaaca tttcttcccg aaatgcggtc
    15661 atttctgggg taacattcgc cagaaccgtt ggcggatagt agttacctgc cccagccatc
    15721 ttttccccgc ccagtaacaa atgcgcaccc tgcgccaggg ttttctccac ctgatgatgc
    15781 agctcatcac gtaaatcaaa acgagccatt ggtccgagag tgttctcttc gtcacgggga
    15841 tcgcccattt tcaaggctgc cgcagctgcc acaaaacgtt cggtaaatgc cgaagcaatt
    15901 ccctcttcga tgataaaacg ttttgccgct gcacaaacct gtccggtatt ctgataacgt
    15961 ccggctaccg ccgctttcac cgccagttcc agatcggcat cgttaagcac aataaacgga
    16021 tccgaaccgc ccagttccag tacgcatttt ttcagtgccg ctccagcctg tgcgccaata
    16081 gccgctccag cacgaacact tccggtcacc gtgatagcag caatgcgcga gtctttaatc
    16141 atctgactga caccgtcgtt gtcggcattc agccagccat atacgccttg tgggataccc
    16201 gcatctttaa acacctgggc aatgagctgt gcacagccca tcacattcgg cgcatgttta
    16261 agtaagtagc cgttacctgc aagaatgatg ggaacagcgc cacgcatcac ctgccataac
    16321 ggaaaattcc acggcataat cgccagaatc gtccccaacg gtcgatactc aataaccgcc
    16381 tgctgatttt ccaccagcgt aggttccgcc ttcagcattg ccggaccatg ttctgcatac
    16441 cagtcacaca aattcgccga tttcgccact tcagcgcgcg cctggttgat tggtttgccc
    16501 atttcgctgg tgatcatttg cgccatttct tcgctacgag cgcgcagagc cttaccgata
    16561 tcacgcagtt tttcagcacg ataatctata tttgtctcgc gccagtcacg aaagcctgct
    16621 gccgccagct gaagtgcgtt ttcgatatca tcagcgccag cccacggcaa cacagaaagt
    16681 tgttcacccg tggcaggatt tatcgaaatt gcatgagttg ccggagtaat ggtcatcggg
    16741 gtatctcctt tatgagtcat ggtatgaaga tacgcagatt tactcttgct ttaaaatgaa
    16801 taatattaag ccacttattc acgaatcgag aatgttatgg atctgaccca actggagatg
    16861 ttcaacgcgg ttgccgaagc tggcagcatt acgcaggctg cagcaatagt gcatcgcgtg
    16921 ccgtcgaatc tcactacccg tctacgccag ctggaaacag aactgggggt agatctgttt
    16981 attcgcgaga atcagcgttt acgtctctct cctgccgggc ataacttttt acgctatagc
    17041 caacaaattc tcgcgttagt ggatgaagcg cggagcgttg tcgctggcga tgagccgcaa
    17101 ggtttatttt ctctgggttc gctggaaagc accgcagcag ttcgcattcc ggccacgctg
    17161 gcggaattca accgtcgtta tcccaaaatt cagttttcac tttccaccgg cccttccggc
    17221 actatgctgg aaggagtgct ggagggaaaa ctgaatgcgg catttattga tgggcccatt
    17281 aaccatactg ccattgacgg gataccggta taccgcgagg agctgatgat cgtcacgcca
    17341 caaggtcatg cgccagtaat ccgcgccagt caggttaacg gcagtaacat ttatgccttc
    17401 cgcgccaatt gttcgtatcg tcgccacttc gagagctggt ttcatgctga cggtgccgct
    17461 ccgggaacta ttcacgagat ggagtcgtat cacgggatgt tggcctgtgt ggtcgcagga
    17521 gcaggcattg cgcttattcc acgctctatg ctggaaagta tgccggggca tcaccaggtt
    17581 gaagcgtggc cgttagctga gcaatggcgt tggttaacaa cctggctggt ctggcgtcgt
    17641 ggtgcgaaaa cgcttccgct tgaggcattt attcaactac tggatgttcc tgactcggca
    17701 aaacagggtt accaatgagc tatttttgat agttcttgca gctcaaatgc cctatatagt
    17761 gatgttccga tgacttatga ctatatgggg caaatatggt tacgccagta agcatcagca
    17821 attacatatc tcttcctgat aattttcccg ctcgtaatat tgcacctcag gtaaaagaag
    17881 ttttaaagga ttttattgat gcactcagta ccataatatg tgatgaagag tggcgcacgt
    17941 ttttaaacat caactcagcc acaaaaaaga tatttaacaa ccttgataac ttatcatata
    18001 ttcagaggac tt
//
LOCUS       DACBVI010000092        17959 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.092, whole genome shotgun sequence.
ACCESSION   DACBVI010000092 DACBVI010000000
VERSION     DACBVI010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.092"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            170..2410
                     /gene="fepA"
                     /locus_tag="IU325_15000"
     CDS             170..2410
                     /gene="fepA"
                     /locus_tag="IU325_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HAP5810314.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            2585..3205
                     /gene="entD"
                     /locus_tag="IU325_15005"
     CDS             2585..3205
                     /gene="entD"
                     /locus_tag="IU325_15005"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HAP5810315.1"
                     /translation="MKTTHTALPFAGNTLHFVKFDPASFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAGVYGSISHCGATALAVVSRQ
                     PIGIDIEEIFSAQTAAELTDNIITPAEHERLAECGLTFSLALTLAFSAKESAFKASKI
                     QAAQGFLDYQIISWNKQQIIIRLEDEQFAVHWQIKEKIVITLCQHD"
     gene            complement(3327..3479)
                     /locus_tag="IU325_15010"
     CDS             complement(3327..3479)
                     /locus_tag="IU325_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HAP5810316.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVRDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            3922..5040
                     /locus_tag="IU325_15015"
     CDS             3922..5040
                     /locus_tag="IU325_15015"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAP5810317.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRSLTEDTLRLLEKIVPSAHKMGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            5106..5354
                     /locus_tag="IU325_15020"
     CDS             5106..5354
                     /locus_tag="IU325_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 family protein"
                     /protein_id="HAP5810318.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            5419..5787
                     /locus_tag="IU325_15025"
     CDS             5419..5787
                     /locus_tag="IU325_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAP5810319.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            5881..6534
                     /gene="nfsB"
                     /locus_tag="IU325_15030"
     CDS             5881..6534
                     /gene="nfsB"
                     /locus_tag="IU325_15030"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HAP5810320.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKMLDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRRFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            6642..7889
                     /locus_tag="IU325_15035"
     CDS             6642..7889
                     /locus_tag="IU325_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HAP5810321.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIFLHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHKEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(7970..9346)
                     /locus_tag="IU325_15040"
     CDS             complement(7970..9346)
                     /locus_tag="IU325_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HAP5810322.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(9448..12591)
                     /gene="cusA"
                     /locus_tag="IU325_15045"
     CDS             complement(9448..12591)
                     /gene="cusA"
                     /locus_tag="IU325_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HAP5810323.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAAIMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(12603..13826)
                     /gene="cusB"
                     /locus_tag="IU325_15050"
     CDS             complement(12603..13826)
                     /gene="cusB"
                     /locus_tag="IU325_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HAP5810324.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFDRAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(13842..14174)
                     /gene="cusF"
                     /locus_tag="IU325_15055"
     CDS             complement(13842..14174)
                     /gene="cusF"
                     /locus_tag="IU325_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HAP5810325.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KTIDLESKKITIHHDPITAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(14198..15580)
                     /gene="cusC"
                     /locus_tag="IU325_15060"
     CDS             complement(14198..15580)
                     /gene="cusC"
                     /locus_tag="IU325_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HAP5810326.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLNQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNVSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            15737..16420
                     /gene="cusR"
                     /locus_tag="IU325_15065"
     CDS             15737..16420
                     /gene="cusR"
                     /locus_tag="IU325_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HAP5810327.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            16410..17858
                     /gene="cusS"
                     /locus_tag="IU325_15070"
     CDS             16410..17858
                     /gene="cusS"
                     /locus_tag="IU325_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HAP5810328.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLKRVLNHPEETQARRLMTLEDIVSGYSNVLISLADSQGKTVYHSP
                     GAPDIREFTRDAIPDKDTRGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEIGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         4374 a         4764 c         4555 g         4266 t
ORIGIN      
        1 ttgcatttgc aatagcgtaa tggcgcgccg tgggaagcgc ggacattaat taaccaactg
       61 cactgcgtgt ctttcaggat caaaggtttt cgcggtagcg ggatgcgtcg tgttgatgac
      121 gaccatgccc gacagttgca attcgtggca aaaatgcagg aataaaacaa tgaacaagaa
      181 gattcattcc ctggccttgt tggtcaatct ggggatttat ggggtagcac aggcacaaga
      241 gccaaccgat actcctgttt cacatgacga taccattgtc gttaccgccg ccgagcagaa
      301 cttgcaggca cctggcgttt cgaccatcac cgcagatgaa atccgcaaaa acccggttgc
      361 ccgcgatgtg tcggagatca tccgtaccat gccaggcgtt aacctgaccg gtaactccac
      421 cagtggtcag cgtgggaata accgacagat tgatattcgc ggcatgggtc cggaaaacac
      481 gctgattttg attgacggca agccggtaag cagccgtaac tcggtgcgtc agggctggcg
      541 tggcgagcgc gatacccgtg gtgatacctc ctgggtgcca cctgaaatga ttgaacgtat
      601 tgaagttctg cgtggtccgg cagctgcgcg ttatggcaac ggcgcggcgg gcggcgtggt
      661 taacatcatt accaaaaaag gcagtggtga gtggcacggc tcgtgggacg catatttcaa
      721 tgcgccagaa cataaagagg aaggtgccac caaacgcact aactttagcc tcaccggtcc
      781 gctgggcgac gaattcagct tccgcttgta tggcaacctc gacaaaactc aggctgacgc
      841 gtgggatatc aaccagggcc atcagtccgc gcgtgccgga acgtatgcca cgacgttacc
      901 agccgggcgc gaaggggtga tcaataaaga tattaacggc gtggtgcgct gggacttcgc
      961 accattacaa tcgctggaac tggaagcagg ttacagccgc cagggtaacc tgtatgcggg
     1021 cgatacccag aacaccaact ctgactccta tacccgctcg aaatatggcg atgaaaccaa
     1081 ccgtctgtat cgccagaact actcgctgac ctggaacggc ggctgggata acggcgtgac
     1141 caccagcaac tgggtgcagt acgaacacac ccgtaactcg cgtattccgg aaggtctggc
     1201 gggcggtacc gaagggaaat ttaacgaaaa agcgacacag gatttcgtcg atatcgatct
     1261 cgatgacgtg atgctgcaca gcgaagttaa cctgccgatt gatttcctcg ttaaccagac
     1321 gctgacgctg ggtacggagt ggaatcagca gcggatgaag gacttgagtt ccaacaccca
     1381 ggcgctcacc gggacgaata ccggcggcgc tattgatggc gtgagtgcca ccgaccgtag
     1441 cccgtattca aaagcagaaa ttttctcgct gtttgccgaa aacaatatgg agctgactga
     1501 cagcaccatc gtaacgccgg ggctgcgttt cgatcatcac agcattgttg gcaataactg
     1561 gagtccggct ctaaacatat cgcaaggttt aggcgatgac ttcacgctga aaatgggcat
     1621 tgcccgtgct tataaagcgc cgagtctgta ccagactaac ccaaactaca ttctctacag
     1681 taaaggtcag ggctgctatg ccagcgcggg cggctgctat ctgcaaggta atgatgacct
     1741 gaaagcggaa accagcatca acaaagagat tggtctggag ttcaaacgcg acggctggct
     1801 ggcgggtgtg acctggttcc gtaacgatta tcgcaataag attgaagcgg gctatgtggc
     1861 tgtagggcaa aacgcagtcg gcaccgatct ctatcagtgg gataacgtac cgaaagcggt
     1921 ggttgaaggt ctggaaggat cgttaaacgt accggttagc gaaacggtga tgtggaccaa
     1981 taacatcact tatatgctga agagtgaaaa caaaaccacg ggcgaccgtt tgtcgatcat
     2041 cccggagtat acgttgaact caacgctgag ctggcaggca cgggaagatt tgtcgatgca
     2101 aacaaccttc acctggtacg gcaagcagca gccgaagaag tacaactata aaggtcagcc
     2161 agcggttgga ccggaaacca aagaaatcag tccgtacagc attgtgggtc tgagcgcgac
     2221 ctgggatgtg acgaagaatg tcagtctgac cggcggcgtg gacaacctgt tcgacaaacg
     2281 tttgtggcgt gcaggtaatg cccagaccac gggcgatctg gcaggagcca actatatcgc
     2341 cggtgccggg gcgtatacct ataacgagcc gggacgtacg tggtatatga gcattaatac
     2401 tcacttctga tgctaacgtc agattgttga caaagtgcgc gtagttcatg ccggatgcgg
     2461 cgtgaacgcc ttatccggcc tacaaaaatc ttgccaattc aatatattgc aggaccacgt
     2521 aggcctgata agcgtagcgc atcaggcagt tttgcgtttg tcatcagtct ctaatatggt
     2581 cgacatgaaa actacgcata ccgccctccc ctttgccgga aatacgctgc attttgttaa
     2641 gttcgatccg gcgagttttt gtgagcagga tttactctgg ctgccacact acgcgcaact
     2701 gcaacacgct ggacgtaaac gtaaaacaga gcatttagcc ggacggatcg ctgcagttta
     2761 tgcgctgcgg gaatatggct ataaatgtgt gcccgcaatc ggcgagctac gccaacctgt
     2821 gtggcctgcg ggggtatacg gcagtattag ccactgtggg gctacggcat tagccgtggt
     2881 atctcgtcaa ccgattggca ttgatattga agaaattttt tctgcgcaaa cagcggcaga
     2941 attgacagac aacattatta caccagcaga acacgagcga ctcgcagaat gcggtttaac
     3001 cttttctctg gcgctgacac tggcattttc cgccaaagag agcgcattta aggcaagcaa
     3061 gatacaggcc gctcaaggtt ttctggatta tcagataatt agctggaata aacagcagat
     3121 catcattcga ctagaggatg agcagtttgc tgtgcactgg cagataaaag aaaaaatagt
     3181 cataacgctg tgccaacacg attaattgac aacatctggt acgattcgcc cgcagccatt
     3241 actgaccgcg ggcgaaagtg taaagcaggt gccttaccat cctgacctga caaccggata
     3301 tgcggggatt gctccccgca caacggctac ttcttcggtt cgtaagcgag aacagcctta
     3361 aactcaatat tacgttcctt caccgtaaac tcacacagcg agtcgcggac cagaagcgta
     3421 aatcccagca ccgttaaaca cagcactatg actgccacaa gggcatattt cgtcagcatg
     3481 tttatattgc ctccatgcga agaagggact accatccaaa ttgtttgggt ttggagtggc
     3541 agccccaggg ttgatagaaa tatcgtcctg gggcttcatc tttccagacc tcagagtaaa
     3601 cctgaaaccg gaaagcttca ggcacccgcc gttatcttac ccttccttag caaaataact
     3661 gtttatttat acagtaagtc ctgtttcgga cttctcaatc ctcatttgat caactgaagc
     3721 tgaacccgtt tttttctgca tgcctcaatc gtcaaaattc ttcgccacca gtccgcactg
     3781 ccataacttg tcatattgca aaatgtagcc aatttgcttc agtcaccatc atcaaaaaaa
     3841 gaaacattcc agaacttccg catctcgccc agtcgctccc acttctatac tgaatagaaa
     3901 acgccaacat aagagaaacc tatgccatta cccgattttc atgtttctga accttttacc
     3961 ctcggtattg aactggaaat gcaggtggtt aatccgccgg gctatgactt aagccaggac
     4021 tcttcaatgc tgattgacgc ggttaaaaat aagatcacgg ccggagaggt aaagcacgat
     4081 atcaccgaaa gtatgctgga gctggcgacg gatgtttgcc gtgatatcaa ccaggctgcc
     4141 gggcaatttt cagcgatgca gaaagtcgta ttgcaggcag ccgcagatca tcatctggaa
     4201 atttgcggcg gtggcacgca cccgtttcag aaatggcaac gtcaggaggt atgcgataac
     4261 gaacgctatc aacgaacgct ggaaaacttt ggttatctca ttcaacaggc gaccgttttt
     4321 ggtcagcatg tccatgttgg ttgcgccagt ggcgatgatg ccatttattt gctgcacggc
     4381 ctgtcacggt ttgtgccgca ctttatcgcc ctttccgccg cgtcaccata tatgcaagga
     4441 acggatacgc gttttgcctc ctcacgaccg aatatttttt ccgcctttcc cgataatggc
     4501 ccgatgccgt gggtcagtaa ctggcaacaa tttgaagccc tgtttcgctg cctgagttac
     4561 accacgatga tcgacagcat taaagatctg cactgggata ttcgccccag tcctcatttt
     4621 ggcacggtgg aggttcgggt aatggatacc ccgttaaccc ttagccatgc ggtaaatatg
     4681 gcaggattga ttcaggctac cgcccactgg ttactgacgg aacgcccgtt taaacatcag
     4741 gagaaagatt acctgctgta taaattcaac cgtttccagg cctgtcgcta tgggctggaa
     4801 ggcgtcatta ccgatccgca tactggcgat cgtcggtcac taacggaaga caccttgcgt
     4861 ttactggaaa aaatcgtccc ttctgcacat aaaatgggtg catcgagtgc gattgaggcc
     4921 ctgcatcgcc aggtcgtcag cggtctgaat gaagcgcagc tgatgcgcga tttcgtcgcc
     4981 gatggcggct cgctgattgg gctggtgaaa aagcattgtg agatctgggc tggtgactaa
     5041 gccgggaatt gccttacaga agaatatccc tgacaatggt gttttaactt acatttaaca
     5101 atgttatgaa acacccttta gaaaccttga ccaccgcagc aggcattttg ctgatggctt
     5161 tcctttcttg cctgctgcta cccgcccccg cactggggct gacgctggca caaaaactgg
     5221 tgaccatgtt ccatctgatg gatcttagcc agctttacac tttattgttt tgtctgtggt
     5281 ttttagtgct gggcgctatt gagtattttg ttctgcgctt tatctggcga cgctggttct
     5341 cgctggcgga ttaaacgtcg attatcgccg tacttcgcgg catactttgc ttattctcct
     5401 tcgcctaaag gaacgtttat ggataagcaa tcactgcacg aaacggcgaa acgcctggcc
     5461 cttgagttac cctttgtcga gctttgctgg ccttttggcc cggagttcga tgtttttaaa
     5521 attggcggca agatttttat gctgtcgtcg gagctgcgcg gcgtcccctt tatcaatctg
     5581 aagtccgatc cacaaaaatc cctgttaaat cagcaaatat atccgagcat taagccaggg
     5641 tatcacatga ataaaaagca ctggatttcg gtgtatcccg gagaggaaat ctccgaagcg
     5701 ttacttcgcg atctgatcaa cgattcgtgg aatctggtgg ttgatggtct ggctaaacgc
     5761 gatcaaaaaa gagtgcgtcc aggctaaagc gggaatctat agcgcatttt tctcgcttac
     5821 catttctcgt tgaaccttgt aatctgctgg cacgcaaaat tactttcaca tggagtcttt
     5881 atggatatca tttctgtcgc cttaaagcgt cattccacta aggcatttga tgccagcaaa
     5941 aaactaaccc cggaacaggc cgagcagatc aaaactctcc tgcaatacag cccatccagc
     6001 accaactccc agccgtggca ttttattgtt gccagcacgg aagaaggtaa agcgcgtgtt
     6061 gccaaatccg ctgccggtaa ttacgtgttc aacgagcgta aaatgcttga tgcctcgcac
     6121 gttgtggtgt tctgtgcaaa aaccgcgatg gacgatgcct ggctgaagct ggttgttgac
     6181 caggaagatg ccgatggccg ctttgccacg ccggaagcga aagccgcgaa cgataaaggt
     6241 cgcaggttct tcgccgatat gcaccgtaaa gatctgcatg atgatgcaga gtggatggca
     6301 aaacaggttt atctcaacgt cggcaacttc ctgctcggcg tggcggcgct gggtctggac
     6361 gcggtaccca tcgaaggttt tgacgccgca atcctcgatg cagaatttgg tctgaaagag
     6421 aaaggctaca ccagtctggt ggttgttccg gtgggtcatc acagcgttga agattttaac
     6481 gctacgctgc cgaaatctcg tctgccgcaa aacatcacct taaccgaagt gtaattctct
     6541 cttgccgggc atctgcccgg ctatttcccc tcagattctc ctgatttgca taaccctgtt
     6601 tcagccgtca tcataggctg ctgttgtata aaggagacgt tatgcaggat ttaatatccc
     6661 aggttgaaga tttagcgggt attgagattg atcacaccac ctcaatggtg atgattttcg
     6721 gtattatttt tctgaccgcc gtcgtggtgc atattttttt gcattgggtg gtactgcgga
     6781 ccttcgaaaa acgtgccatc gccagttcac ggctatggtt gcaaatcatt acccagaata
     6841 aactcttcca ccgtttagct tttaccctgc aggggattat cgtcaatatt caggcggtat
     6901 tctggctgca aaaaggcacc gaagcggcag atattctgac tacctgcgcg cagttgtgga
     6961 tcatgatgta tgcgctgctt tcagtcttct cgttgctgga tgttattttg aatctggcgc
     7021 agaaattccc ggcggcatct cagttaccgc tgaaagggat atttcagggg attaaactga
     7081 tcggcgcgat tctggtcggc attttgatga tctcgctgct gattggtcag tcgccagcga
     7141 ttctgatcag cggtcttggt gcaatggctg ccgtgctgat gttggtattt aaagatccga
     7201 ttcttggtct ggtggcaggt attcagcttt ccgcgaacga tatgctgaaa ctgggcgact
     7261 ggctggagat gccgaaatac ggcgcggatg gcgcggtgat cgatattggg ttaaccaccg
     7321 tcaaagtgcg taactgggac aataccatta ccactattcc cacctggtct ctggtctctg
     7381 actcctttaa aaactggagc gggatgtcag catctggcgg gcgacgtatt aagcgcagta
     7441 tcagtattga tgtcaccagt attcgttttc ttgatgaaga cgaaatgcaa cgtctgaata
     7501 aagcgcattt gttaaaacct tatttaacca gccgccacaa ggaaattaat gagtggaatc
     7561 gccagcaagg ttctacggag tcggtattaa acctgcgccg aatgaccaat attggaacct
     7621 ttcgtgccta tctgaacgaa tatctgcgga atcatccgcg gattcgcaaa gatatgacct
     7681 taatggtgcg ccaattagct ccgggcgata acggtttacc gcttgagatc tacgcgttta
     7741 ctaataccgt ggtgtggctg gaatatgaaa gcattcaggc tgatatattc gatcacatat
     7801 ttgcgattgt cgaagagttt ggtctgcgac ttcatcagtc gccaaccggc aatgatattc
     7861 gctctctggc gggtgcattt aagcagtaat taaaaaaacc gctcttatcg aatggatgag
     7921 agcggtttcg gatggttgac atcattttgt cggatacggc gtgaatgcct tatttccgac
     7981 gcagcgtttt aaacgctaca aacaggaata caatccacac tggcagcaga atcgctgaca
     8041 agcgcatatc atccatcgtg cacatcagca gcaaaatcat gccgaggaag gcaatgcaga
     8101 gatagttgcc gaacggataa agcagcgcct taaactgtgt ttcacgtccc tgacgtcgca
     8161 tcgccgcgcg aaaacgcaga tgcgccaggc agatcataat ccagttcaac agcagcgttg
     8221 ctaccaccag cgccatcagc agaccaaacg ctttttgcgg cagcagatag ttgattaaca
     8281 ccaccagcga agtgatcgct ccggaaagca tcagcgagtt aatcggcacg ccgcgacggc
     8341 tgacgcgagt caaaaacttc ggcgcattac cctgcacaga aaggccaaac agcatgcggc
     8401 tgttagagta aaccccgctg ttatacaccg ataatgatgc taccagaatg acgaagttca
     8461 gcgcagaagc taccacgttg ctgtcgagat tatggaaaat catcacaaaa gggctactgt
     8521 tggatttcac ttccacccac ggatagagtg ccagtaaaac caccagtgaa ccgatataaa
     8581 acagcaggat gcgatacacc acctgattca ctgctttcgg gatgcttttt tccggatcgc
     8641 gcgcttcagc cgcagtaatc ccaatcagct ccagaccgcc gaaggagaac ataattaccg
     8701 ccagcgacaa aatcagccca ttccagccgg tggcgaagaa accaccgtag cgccagaggt
     8761 tgtcgatact ggctttttcg ccgccgtgcc cagaaaacag cagccacagg ccaaagccga
     8821 tcataccgat gatcgccagc actttaatca gcgcaaacca gaattcggtt tcgccatata
     8881 agcgcacgtt taccaggtta acggcgttga tgataataaa gaaggcggca gcccaaatcc
     8941 acgttggaac atccgggaac cagtactgca tatagatgcc cgcagcggtc agctctgcca
     9001 ttcccaccag cacgaacatc acccagtagt tccagccaga gaggaagccc gcaaacggtc
     9061 cccagtattt ataggcaaag tgggcaaatg aaccagatac cggctcttcg accaccatct
     9121 cgccgagctg gcgcataatc aggaaagcga tgatcccggc gacgccgtag cccagcaata
     9181 cagccggacc cgccatctga atcgccgggc caatgccaag aaacagacca gtaccaattg
     9241 cgccacccag cgcaatcagt tgaatatgac ggttatgtaa tccgcgatga agcgtcggct
     9301 cttgattcga cgcagtatct tccgatacgg ttgacgcgtt tttcacgcct ttcccctgtg
     9361 tgtctttttt gttgaggggc accttttaac atttagtgcc catcgtcgca agacacaatc
     9421 cacacggtta aaccgggtat cctgctttta tttccgtacc cgatgtcggt gcagccacat
     9481 cagcttatat gccgccggga taataaacag cgatagcaaa ggcgcggtga tcatgccgcc
     9541 aatcatcggc gcggcaatcc ggctcatcac ctctgaacct gctcccgttc cccacagaat
     9601 cggcagcaga ccagcgataa tcaccgccac cgtcatcgct ttcgggcgca cgcgcaggac
     9661 cgcgccgtga tacaacgcct catccagctt ctgctcgctg aatgtttgcg gattattcaa
     9721 cgacggctcg gcctctatgg cgtgacgtaa atacatcagc atcaccacgc caaattcggc
     9781 ggcaaccccg gcgagggcaa taaaaccggt gcccgtcgcc acggaaagat gaaagcccat
     9841 ccaccacagc aaccagatgc cgcccaccag cgcaaacggt acgctgctga taatcagcaa
     9901 cgcttcgccc acccgacgga acgccagata taacagcacg aagatgatca tcagcgtcat
     9961 cggcaccatg agtttcagct tatggttggc gcgttccagt agctcgaact gcccggagaa
    10021 tgccacgctg gtgcccggtt ttaactgtac tttttcagct atcgcttttt gcagatcgtg
    10081 aaccaccgac accatgtcac gatcgcgggc atcgatataa atccagctcg tcgggcgcgc
    10141 attctcggtt ttcagcatcg acggtccggt agagacttta acgtcggcca cgtctgccag
    10201 ggtgatttgc tgcttcatcg gcgtcaggat cggcaactgg cgcagcgcct gcggactatc
    10261 gcgccagctt tgcggataac gcagattaat tggataacgg gcaatccctt ccaccgtttc
    10321 gccaaccatc gccccgccca ccgcagaagt cacaaacaat tgcacatccg ccaccgtcat
    10381 accgtaacgc gcggcttttt cacggttaat ctcaacgttg atatagcgcc caccttccag
    10441 acgctcagca agcgcagaag ctacgcctgg caccgttcgt gccacttctt caatttgctc
    10501 agccatcgcg tcgatatccg ccagcacagt accggaaact ttaatgccga tggggctttt
    10561 aatgccagtc gagagcatat cgatacggtt acgaattggc ggcacccaca gattcgccag
    10621 ccccggcagc cgcacggtgt tatccagttc ctcgatgatt ttgtccatcg tcatgcctgg
    10681 ccgccactgc tcttgtggct taagctggat ggtcgtttct accatctcca gcggagcaga
    10741 atcggtggcg gtttccgctt tgccggtttt gccaaatacc cgcgccactt caggtacgct
    10801 cataatcagc ttgtcggttt tttgcagcat aatcgccgcc tctgctgcgg aaatccccgg
    10861 cagcgtcgat ggcatataca acaagtcacc ttcattgatc tgcggtaaaa actccccgcc
    10921 aactttattg agcggccaga gaaccgtcag caccgaaagc gctgccacca gcagcgtggt
    10981 tttcggccag tgcagtactt tcagcaacag cggatgataa acacgaatca aaaagcgatt
    11041 gagcgggtta ctgctttccg gcggaatttt gccacggatc cagtagccca tcaggatagg
    11101 gatcactacg atcgccagca gcgccgcacc cgccatcgca tacgttttgg tgaacgccag
    11161 cgggccaaac agacgccctt cctgcccttc cagggtgaag atcgggataa acgacaacgt
    11221 gataatcagc agactgataa acagcgccgg ccccacttca acagatgcat cggtgatcac
    11281 ctgccagcgc gttttattat ccagcgtggc gtcaggatgc tggtgctgcc actcttccag
    11341 ccgtttatgc gcattctcga tcatgacgat agcggcatcg accatcgccc cgacggcaat
    11401 agcaataccg cccagcgaca taatattggc gttcagtccc tgaaagtgca tgacaataaa
    11461 agcaatacac aaccccagcg gcaacgaaat aatcgccacc agcgccgagc gcacatgcca
    11521 gagaaacagc gcacagacta ccgccaccac aataaactct tccagcaact tgccgctgag
    11581 gttgtcgatg gcgcggtcaa tgagctggct gcgatcgtat gttgtaacta tctccacgcc
    11641 ttccggcaga ctacttttca gcgtttccag tttgtctttc acggcggcga tcacttctcg
    11701 ggcgtttttg ccggagcgca ggatcaccac cccgcccgcc acttcgcctt cgccgttcag
    11761 ttcggcaatg ccccggcgca tttccgggcc aatctggacc ttcgcaacat cacgcagata
    11821 aacgggcacg ccattttcac tggcttttaa aacgatgtga ttaaagtcgt cgagcgtttg
    11881 cagatagccg ctggctcgca ccatatattc cgcttccgcc agttcgatcg acgaaccgcc
    11941 cgcttcctgg tttgaagcat ccagcgcgct ttttacttcg gcgagactga tgccatattg
    12001 cgccaggcgc tgaggatcga taaccacctg atactctttc accacaccgc ctaccgacgc
    12061 cacttccgca acgtcaggaa tggttttcag ctcatatttg agaaaccagt cctgcaatga
    12121 gcgtaaatcg gccagatcgt gcttaccgct gcgatccacc agtgcatatt catagatcca
    12181 gccaacaccc gtggcatctg gccccaactc ggcgctgact cccgcaggca gcttaccctg
    12241 tacctggttg aggtactcca gcacccgcga gcgcgcccag tacggatcgg tgccatcttc
    12301 gaaaatgaca tacacataag agtcgccaaa ctgcgaaaaa ccgcgcacag tcttcgcgcc
    12361 aggcaccgac aacatggtgg tggttagcgg ataagtcacc tgattttcaa cgatttgcgg
    12421 tgcctgaccg ggatagctgg ttttaataat cacctgcaca tcggagagat ccggcagcgc
    12481 atccactggc gtattaatga tggtccaggt gccccagata ctcagaaata acgcgcccat
    12541 cagcaccaga aaacggttcg ccaccgagcg acgaataatc cattcaatca ttggttattc
    12601 cctcaatgtg catgggtagc actttcagag cgcatccgct ccagtgcgcc agaaatattg
    12661 gcttcggaat caatcaggaa caggccgctg gaaaccacct tttcaccttc cgccagacca
    12721 gagcgtaacg cggtgacgcc ttgcgatgcc tggaaaacgg caacgcgttt cggtacaaag
    12781 cgcccgtcgg catcaacggt aatcacccgc tgttcgttgc cggtatcaat cagcgcctgt
    12841 gacggaatga gcagcatcgg ttcgctggcg gtgttgagtt gcaaccaggc gttcattccc
    12901 ggttttagcg cctcgtcggc gttgtcgact tccagacgca gctgcaacgt gcgggtcgcg
    12961 gcatccacgc caggtagtag cgtccatttg cggatggtga gtgttttatc aggtcgcgcc
    13021 ggaacggtga gcgtaaactg cgaggcatct ttcaccagcc aggcgatgga ctccgggatc
    13081 gcagcagtga cccacaccgg gtccataccc tgaattttcg cgaccacgtt atctttggcg
    13141 atattcattc ccgcgcgcag atcaaacgcg gtgatcacgc catcaatggg cgctttgagc
    13201 gtaaagcgag tctgtatttt ttgcgtggcg atcaggcggc gaatatccgc ctccggcatt
    13261 cccgccagtc tcagccgctc aagaatgcct tcagtctggg tcgccgtacc gccggtttcg
    13321 cgcagcagta aatactcact ctgcgcttcc acccagtcgg gaatggtcag gtcgagaagc
    13381 ggtgtgccct tttgtacttt atcgcccacg gtaagcggat acaccttgtc gataaacccg
    13441 gcagcgcgag cctgcacaat ggcatactga tactcgttgt aactgacatt cgccgggaaa
    13501 ctctgggcaa aagtcagcgg tccgcgcgtg acggtagccg ttttcacccc cagattctgc
    13561 gtctgagtcg ggtcaatgcg cacaccagac gcagaactct cttcatcggc atatttcggc
    13621 accagatcca tatccataaa cggcgattta cctggtttat cgaaccgcgt attgggatac
    13681 atcgggtcgt accagaataa gattttacgt tctgcggtcg acgttttttc tgcgggcggt
    13741 tcagcccggt caaaccaggt aaaacctgcc gcagaaataa taccgcccgc gatcatgctg
    13801 ccgataataa gcgcgatttt tttcatctca ttaaacctgg gttactggct gactttaata
    13861 tcctgtaata aagaaaggtt gccctgctgg acaaaattaa acgccacttt gtcgccagtt
    13921 ttaatttcac tcattttcgt ctgcggagtg atggtaaagc gcatggtcat ctccggccag
    13981 ttcaccgcgg taattggatc gtggtggatg gtgatttttt tgctttccag atcaatggtt
    14041 tttaccaccc cagtggcgct aatgacctgt ggttgtgcct cgctcatggt ttcatgatga
    14101 tgttcgttag cctgggcatt aaagccaatc acggtaaaca gactgaacat tgcgacttgc
    14161 agtgcttttt tcatttattc tctcctggag ttaaaaatta ttgctgccaa ccgccaccaa
    14221 gtgcggtata taaagaaatt tcgttgacct gacgggcata attcaggtca agtaaggttt
    14281 gtcgggttgc aaataaagaa cgctcggcat ccagcacttc cagataactg actgcgccgt
    14341 gctgatataa tgcccgcgcc cgttgcaaag taatttgcag cgacgccaga taacgctgct
    14401 gggcgctgat ttgatcgttc aggctttgac gtaatgccag cgcatctgcc acttctttaa
    14461 aggcgttctg gattttctgt tcataattca ctaccgactg ctgctggcga atttcggcga
    14521 tatccagatt ggcctggttg cgtccggcat taaaaatggg gatctcaatt ttaggaataa
    14581 aattccacat cccgctgctg gcgttaaata atgacgacag atcgctgctg gcggttgata
    14641 ttccgctggt caggcttata gatgggaaaa atgccgcgcg tgcggcccca atattggcat
    14701 tagccgccat taacgcgtgt tcagcttcca taatatccgg gcgctgcaat aagatttgcg
    14761 acgacaagcc cgccggtaat ttaacgcttt gcaggctgtc gctgtttact gtctgcgctt
    14821 gcggcagctt gccgtagctt cccaataata gttgcaatgc attattcgcc tgcgccagtt
    14881 ccccctgacg tttagcgatg tcgctgcggg tactttcaat caccccgcga gcctgttcca
    14941 gcgccagaac attgctgcta ccggtcaaca gttgtttttc gacaaacgca tatgactgct
    15001 gataattacg cagcgtttct tcggctattt gcaattgcgc atacgccagc tgctgattga
    15061 aatagctttg cgcgacatta gaaaccagca gaatatgcac cgcgcgctgg gcttcctcag
    15121 tggctaaata attttgtcgc tcggcttcgc tcacgttctt taagcgaccg aaaaaatcga
    15181 gatcaaagct ggcgttaagg ccagtcgaga actcccgcgt cgtggctgaa tcgcctttaa
    15241 gattgccgct ccagctgccg ctgccctcgc cattgagctg tgggtagcgg tcggcatcgg
    15301 tcagacgata ttgcgcccgc gcttcctgca ctttcagcgt cgccatgcgc aaatcccggt
    15361 tattcaccag cgcctcgcta atcagcgtct tcacctgatt atcaacaaaa aaggtgcgcc
    15421 agcccgcgtt ctggtagtta tctgctgcgt taaccaggcc gttctggttg agtgagaact
    15481 gctgcggcac gggcattgcc gggcgctgat aatccggtgc cagtgaacaa ccggttagcg
    15541 caagggccac acaaaatggc agaagtttac aaggagacat aggctcataa tttctggtga
    15601 ttttatgccg ccaactttac tcgccaggct ctgattttcc ggtgacagga aaatgacaaa
    15661 attgtcattt tgccaataag cgattgccat ctgatcccgc tactctagaa ttgcccgggc
    15721 aacatgcgga ggaaatatga aactgttgat tgtcgaagat gaaaagaaaa ccggagaata
    15781 cttgaccaaa gggttaaccg aagccggttt tgtggtcgat ttggccgaca atgggctgaa
    15841 tggctaccat ctggcgatga ccggtgatta tgacctgata atcctcgata ttatgctgcc
    15901 ggacgtgaac ggctgggata tcgtgcgcat gttgcgctcc gccaataaag ggatgccgat
    15961 tctgttgctt accgcgcttg gtaccattga acatcgcgtc aaggggctgg agttgggggc
    16021 ggatgactac ctggtgaagc cgttcgcttt tgctgaactg ctggcgcggg tgcgcactct
    16081 actgcggcgc ggggcggcgg taattatcga aagtcagttt caggttgccg atttgatggt
    16141 cgatctcgtc agccgcaaag tcacccgtag cggcacgcgc atcacgttga ccagtaaaga
    16201 atttactttg ctggagtttt tcctccgcca tcaaggcgaa gtgctgcccc gctcgcttat
    16261 cgcctcgcag gtgtgggaca tgaatttcga cagcgatacc aacgccattg atgtggcagt
    16321 gaagcggctg cgcggcaaaa tcgacaacga ctttgagccg aagctgattc aaaccgtgcg
    16381 cggcgtgggt tacatgcttg aggtgccgga tggtcagtaa gccatttcag cgcccgtttt
    16441 cgctggcaac ccgcctgacc ttttttatca gcctggccac catcgcggcg tttttcgcct
    16501 ttgcctggat catgattcac tcggtaaaag tgcattttgc tgagcaggat attaatgatt
    16561 taaaagagat tagcgccaca ctcaagcgcg ttcttaatca tcccgaagag acacaagccc
    16621 gacgcttaat gacgctggaa gatatcgtca gtggttattc caatgtgttg atttccctgg
    16681 cagatagcca gggtaaaacg gtgtatcact cccccggtgc gccggatatc cgcgagttta
    16741 cgcgtgacgc catacccgat aaagacacgc ggggcggcga ggtgtatctc ctttccggcc
    16801 cgacgattat gatgccgggc cacggtcacg ggcatatgga acacagcaac tggcggatga
    16861 ttaacttgcc ggttggcccg ttggtggacg gcaaaccgat ttatacgctc tacatcgcgc
    16921 tttcgatcga ttttcatctt cattacataa atgatttgat gaataaactt attatgaccg
    16981 catcgatcat cagcatcctg atcgtcttta tcgtgctgtt ggcggtacat aaaggtcacg
    17041 caccgatccg cagcgtcagc cgtcaaatcc agaacattac ctcgaaagat ctcgacgttc
    17101 gccttgaccc gcagaccgtg ccgattgagc tggaacagct ggtactgtcg ttcaaccata
    17161 tgatcgagcg tattgaggat gtctttactc gccagtccaa tttctcagcg gatatcgctc
    17221 acgaaattcg tacaccaatt acgaatctca taacgcaaac cgaaatcgcc ctcagccagt
    17281 cacgcagcca gaaggagctg gaagatgtgc tctactctaa tctcgaagag ctgacgcgaa
    17341 tggcgaaaat ggtcagcgat atgctgtttc tcgcccaggc cgataacaac cagctaatcc
    17401 ccgaaaagaa aatgctcaac ctggcggatg aaatcggcaa agtgttcgat tttttcgagg
    17461 cgctagcgga agatcgcggc gtggaactgc ggtttgttgg tgacgagtgt caggttgcgg
    17521 gcgatccgct gatgctgcgt cgggcgttaa gcaacctgct ttcgaacgcc ctgcgttata
    17581 cgccgcccgg agaggcgatt gtagtgcgct gccagacggt cgatcaccag gtgcaagttt
    17641 ccgtcgaaaa ccccggtacg cccattgcgc ccgagcactt accgcgattg tttgaccgtt
    17701 tctaccgcgt tgacccctcc cgccagcgaa aaggcgaagg tagcggtatt gggctggcga
    17761 tagtgaaatc gattgttgtc gcgcataaag gcacggttgc agtaacgtca gatgctcggg
    17821 ggacaaggtt tgtgattatg ctgccggaga gagagtgatt attaagttca aattcagggc
    17881 gtggtgcatc acctcctggt attgttgatc aacaaattag agaaaaactt attaaacata
    17941 tgaaatatcg aaggctttt
//
LOCUS       DACBVI010000093        17955 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.093, whole genome shotgun sequence.
ACCESSION   DACBVI010000093 DACBVI010000000
VERSION     DACBVI010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.093"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(513..1514)
                     /locus_tag="IU325_15075"
     CDS             complement(513..1514)
                     /locus_tag="IU325_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP5810329.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGKGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWVTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIEKPVVNCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSEWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQMLF"
     gene            1622..1918
                     /locus_tag="IU325_15080"
     CDS             1622..1918
                     /locus_tag="IU325_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HAP5810330.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(2041..2466)
                     /gene="arsC"
                     /locus_tag="IU325_15085"
     CDS             complement(2041..2466)
                     /gene="arsC"
                     /locus_tag="IU325_15085"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HAP5810331.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIVYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQQGAFTKEDGEKVVDEAGKRLK"
     gene            complement(2479..3768)
                     /gene="arsB"
                     /locus_tag="IU325_15090"
     CDS             complement(2479..3768)
                     /gene="arsB"
                     /locus_tag="IU325_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HAP5810332.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALLTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYALVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFAVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLAENGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3823..4176)
                     /gene="arsR"
                     /locus_tag="IU325_15095"
     CDS             complement(3823..4176)
                     /gene="arsR"
                     /locus_tag="IU325_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HAP5810333.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPSWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSEDSKNICS"
     gene            4919..5002
                     /locus_tag="IU325_15100"
     CDS             4919..5002
                     /locus_tag="IU325_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HAP5810334.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(5056..6408)
                     /gene="gorA"
                     /locus_tag="IU325_15105"
     CDS             complement(5056..6408)
                     /gene="gorA"
                     /locus_tag="IU325_15105"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP5810335.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(6480..7322)
                     /gene="rlmJ"
                     /locus_tag="IU325_15110"
     CDS             complement(6480..7322)
                     /gene="rlmJ"
                     /locus_tag="IU325_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HAP5810336.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDNRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            7525..9567
                     /gene="prlC"
                     /locus_tag="IU325_15115"
     CDS             7525..9567
                     /gene="prlC"
                     /locus_tag="IU325_15115"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HAP5810337.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            9575..10327
                     /gene="rsmJ"
                     /locus_tag="IU325_15120"
     CDS             9575..10327
                     /gene="rsmJ"
                     /locus_tag="IU325_15120"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HAP5810338.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(10376..11845)
                     /gene="dtpB"
                     /locus_tag="IU325_15125"
     CDS             complement(10376..11845)
                     /gene="dtpB"
                     /locus_tag="IU325_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HAP5810339.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGLSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(12161..12595)
                     /gene="uspA"
                     /locus_tag="IU325_15130"
     CDS             complement(12161..12595)
                     /gene="uspA"
                     /locus_tag="IU325_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HAP5810340.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12986..13321
                     /gene="uspB"
                     /locus_tag="IU325_15135"
     CDS             12986..13321
                     /gene="uspB"
                     /locus_tag="IU325_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HAP5810341.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(13392..14891)
                     /gene="pitA"
                     /locus_tag="IU325_15140"
     CDS             complement(13392..14891)
                     /gene="pitA"
                     /locus_tag="IU325_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HAP5810342.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            15123..16325
                     /locus_tag="IU325_15145"
     CDS             15123..16325
                     /locus_tag="IU325_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HAP5810343.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVEKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(16640..17692)
                     /locus_tag="IU325_15150"
     CDS             complement(16640..17692)
                     /locus_tag="IU325_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HAP5810344.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGIPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     SSKEHWLWCYWVIILGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4687 a         4501 c         4585 g         4182 t
ORIGIN      
        1 ttttcttcta cacacaataa atgtaatgcg ttatctcttc ctgatataat tacctttgat
       61 ttccctttta tctccacccg gtaatatttg tgtataaaaa aagggcagtg atagtatcgt
      121 aaaggaaaac gttaacaact atcaaaaagc gctcatttta aaaagttatg atgttcaccg
      181 atagtgtgtg aagtgatatt tttgtgatgt tttagatgtg taataatgaa ccaggttaaa
      241 gtgtatttaa aaatgtgttg tattggcgac tgaaatctgc cctttaaaag ggggatagca
      301 ctgaaacaaa gagacattgc ttctccgata aaatgtcaga gaagcatgat tgcgtggtga
      361 ttgtcgattg atgacttact gacggatccg tcatcattct caaagcatta gttaagttat
      421 ttgtgatttt taaggattat cctatctatg attttctact cagaaggata aggcactcac
      481 aatgctcctt atagtttact gctggcatct atttaaaata gcatttgagt caaatagccc
      541 atcaagatag ccatacctaa gataaccgtc aggaatgcag ctatcatcgg cattctgaac
      601 attgatttaa gtaatatcac ttccgtcagg cttgctccgg cactgccgat gattaatgcc
      661 attaatgctc ccagccccat tccttttgtc atcagaacag atgccagagg gataaccgcc
      721 tctgcccgga tatacagcgg aattccgaca acggcgctca atgggatggc gaagggatta
      781 tctgcccctg catgagcggc aatccactct gagggaataa agccataaat aaaagagcct
      841 attaataccc ctaacaaaag atatggcagt acatctttaa actgttgcaa cgcatctttg
      901 gtggctagtt ttatgggatt aatacttacc acagctttgg tactgcaaca gtttactaca
      961 ggtttctcaa tggctttagc cgctggacta cagcaactca cttttattgg cgtatatgtc
     1021 gtccccggcg aagttttggc tggagcacaa caatttgctg atgagctttt actggcaatg
     1081 atatgacgtt caaatcccag ggaatccagg ataatactgg caagtacgga gacgccggcg
     1141 gcgataatcg cgtacaacaa ggtaactttc cagccaaagg tcacccacat taacccgacg
     1201 ataatgggat taagtaatgg ggaaacaaat aaaaaagtga gagtcggacc aaacccggct
     1261 ttcgctgata acaatccacg taacatgggg attgtcgagc aactacagaa cggggtaacg
     1321 gctcccaaca gagcagccag gagatagcct ttcccttttc ttgcccccat catctgctgg
     1381 attttatggt ctggcacctt ttgtcttatc aggctgacac cggcactaat cactataaac
     1441 agcaaagaaa gctcgacggc gagaaacaca aacatctctg cggcatcttg cagcatcgca
     1501 agccaactac tcatacgtaa cctcattgaa tgaatccata tttccagaat aatcgaaatg
     1561 aaattcagat caagatattt ctggtattat cgaaatatca accagacaaa gggggtgtca
     1621 tatgcagttg gaagaagttg ctaaagcgct gaaagagcta ggtcacccga cccgtttgtt
     1681 catattcaaa cacctggtca aagcggggga acaaggtttg ccggtcggtg agttgcaaaa
     1741 gcagcttggg atccccggct ccacactcag tcaccatatt tcagcactgg tatcggtagg
     1801 gctggtcaaa caaaaccgtg agagtcgaac gctgatgtgt gtttcgcaat acgcaatgct
     1861 ggaagcgata attgaattct tacgtgagga gtgctgcgta aatagcaaaa ttgcctgatg
     1921 cgctacgcgc tacagatttc atgccatgca cggttttgta ggtcggataa gacgcgttag
     1981 cgtcgcatcc gacataaaca aagcgcactt taccaactat gggcagtgga tatcgccgct
     2041 ttatttcaag cgtttacccg cttcatcaac cactttctcg ccatcttcct tggtgaacgc
     2101 gccttgttgc gcatctggca gaatttccag cacgacttct gaagggcggc acaggcgagt
     2161 tcccagcggc gtcaccacaa tcgggcgatt aatcagaatc gggtgctgaa gcataaagtc
     2221 gattaaccga tcgtcagtaa atttatcttc tgcaaggccc agttcctcat acggctcgac
     2281 gtttttacgc agcagcgcgc gtacggaaat ccccatatcg gcaatgagtt tgaccagttc
     2341 atcacgcgtc ggtggggttt ccagataata gacaatggtc ggttctgtgc cgctgttgcg
     2401 gatcatctcc agcgtatttc gcgacgtgcc gcaggctggg ttgtgataaa tggtaatgtt
     2461 gctcatatca atatctcatt acaaagtgaa agagagacgt agcgccagcg cagccagcgt
     2521 cacaaaaagc accggcaggg tcatgataat ccctgtacgg aagtaatatc cccagctgat
     2581 agtcatattc ttctgcgaaa gtacgtgcag ccagagcagc gtcgccaggc taccaattgg
     2641 ggtaattttc ggccccaaat cgcagccaat cacgttggca taaaccatcg cttctttgat
     2701 aacgccagat gccgtgctgc catcaatgga caacgcgcca accagtaccg tcggcatatt
     2761 gttcataata gaagagagga aggcggtgag gaatccggtg ccgagcgtcg cggcccacag
     2821 gccgttttcc gccagcacgt tgagtacgcc agaaagatat tccgttagtc cggcattgcg
     2881 caggccataa accaccagat acatgccgag cgagaagatg acaatctgcc agggcgcacc
     2941 gcgtagcacc ttacccgtat taattgcatg accgcgttta gcgacggcaa ataatatcag
     3001 cgcgcccact gccgcaatgg cgctgaccgg aatgccgagc ggttccagaa cgaaaaatcc
     3061 caccagcaga agcagtaaaa caacccagcc agttttgaac gtagcaggat ctttaatcgc
     3121 ttctgcggga gatttcagca gcgccatgtc gtagttctgc ggaatatctt tgcggaaata
     3181 gagatgcaac atcaccagcg tggcaacaat cgcggcgata tccaccggca ccatcaccaa
     3241 ggcatattcg cgaaagccga ggccaaagaa atctgcggaa acgatattca ccaggttgga
     3301 gacaataagc ggcaggctgg cggtatcggc aatgaacccg gccgccatca cgaacgcaag
     3361 cgtcgtgcct ttactgaacc ctaaagccag cagcatggcg atgacaatcg gtgtcaaaat
     3421 aagcgccgcg ccatcattgg caaacagagc tgcaacggca gcaccgagca ggacaatcca
     3481 ggtaaacagc aagcgaccgc gtccattgcc ccagcgtgag acgtgcagcg ccgcccattc
     3541 aaaaaagccg gactcatcca gcagcaggct gatgataatg acggcaataa acgccgccgt
     3601 cgcgttccag acgatattcc acaccaccgg aatatcaccc ggatgaacca cgcccgtaag
     3661 caacgccagt accgcgccga gcgttgcact ccagccgatg cctaaacctt tcggctgcca
     3721 gataaccaat acgatggtca ggacaaaaat agcgcctgcc agtaacataa tgcctcccgg
     3781 ataaaacaca tctgaaaatt catatgtgtt tagataaatt ttttaactac aaatgttctt
     3841 actgtcttcg gaacaatttt gtcgagccag gttgcggaca atcgcctgaa ccttttcctg
     3901 ttcacatcgc caggcctcat caataatttt cgcggcccat gatggaatat gcggtgataa
     3961 gcggtaatga acccacttac cctgcttgcg atccagcaat agcccacttt cacgcagcaa
     4021 tgccaggtgg cgggatatct tgggctgcga ctgatcgagg gcagtacaga gatcgcagac
     4081 gcataattct cccagctcgc tgagcagtaa aacgatgccc agacgggttt catcagcaag
     4141 aattttgaac agttggatgg gtaacagaaa tgacatattg cgctcctgat tgttgcaggt
     4201 agtgtctctc ttcgaagcgg ataagtcaaa aacatatatg acttaacgaa tgtgtaagcg
     4261 cagaagaagg taataggtgt gaattttgag ttgggtattc atttgaaagg aggtctgaat
     4321 caggacgtaa aaaaaccgac tttgcgtcgg ttttttactt tccagccctg agttggtggc
     4381 tctggccgga gtgagaatgc ccacatctaa cggttcaata ctattagcca gtggctaaca
     4441 ttgcaagcgt tagcgatttc ttcagctggc gaagggacag tttgctatac atgttggtga
     4501 aagaggattt atatactttc atcgggtcgc tgccgtacca ggccttgtcc aaatccggac
     4561 gtaaaaaaac cgacttcgcg tcggtttttt actttgcagc ccagagttgg tggctctggc
     4621 tggagtgaga atgccatcat ctaacgcatc aatgctaata ccatactgaa actattgcaa
     4681 ggacatgctg gttttataac ctgtatgtac tgtatgatta tccagttagt tctgaggcat
     4741 tttcactctg gtaatgcgca taaacgcttt caaagtcctg gtcagaagta cgggtggtgc
     4801 cgttaactga tgctctggtc ggagtgagaa tgcccacatc taacaatggg gcatgcgccg
     4861 tgacaggcag tggatgagta agcggatgca ttctcactcc atcgcatgga gaaaacgggt
     4921 gattgataaa gcaatcatcg ttcttggggc gttaattgcg ctactggaac tgatccgctt
     4981 cctgcttcag cttctgaact gatagcggaa acgtagttaa gggcaaagag cacaccactc
     5041 tttgcccttt aacatttaac gcatggtcac gaactcttct gccgccgtcg ggtgaatggc
     5101 gacggtattg tcgaagtctt ttttggtcgc ccccatcttc agcgccaccg cgaagccctg
     5161 caacatttca tccataccaa aaccaatgcc gtgaattccg acaatcttct cttccggtcc
     5221 aacgcatacc agtttcatgc ggcacggctg gcggtgagtg gtgacggcgg tatacatcgc
     5281 ggtgaaagag gatttataca ctttcacctg atcgtcgccg tactgctcgc gcgcctgcgg
     5341 ttccgttaaa ccaacagtac caatcggcgg atggctgaag accacggtcg gaatgttgct
     5401 gtaatccaga tgctcatcag gcttgttatt aaacaggcgt tcagagagac ggcgacccgc
     5461 tgcaactgcc accggtgtca gttccactgc ccccgtgtta tcgcccacag cgtaaatacc
     5521 ttcaacgttg gtattttgat atttatcgac gacgatatag cctttttcgt tagttttaac
     5581 gccagcggct tccaggttga tgttgtcatt ggcaggctcg cgaccaatcg cccaaatcag
     5641 gcaatctacc gtttcactgc gaccatcttc cagctccagc gtcaggctgc cgtcggcatt
     5701 tttcaccacc gctttcggga tggcgttggt gtgcagctgc gggccttcgg cgttcatcac
     5761 ttcgaccagc gtttcagaga tcatcgggtc gaagctgcgc agcggcgcat gtttacgcac
     5821 aaacagatgc gttttcgcgc cgaggccgtt aatcacgccc gccagctcaa cggcgatgta
     5881 acccgcgcca acaaccgcca cgcgctctgg caaagcagga agggcgaaga agccatcaga
     5941 atcaataccg tattccacgc ccggaatatc cgggtggctc ggacgaccgc cggtggcgat
     6001 cagaatatga tcggccgtga tggtttcgcc gtttacctcc agcgttttgg catcaacgaa
     6061 gcgggcaaag cctttgatta catcaacgtt atttttaccg agcacgtttt cataggaagt
     6121 atgaatacgg tcgatatagg cggtacggct ggcgatcaac gtttcccagt tgaatttatt
     6181 tatagtggta tcgaaaccat aatccggacc gtacatgtgg atcgcttcac ggatttgcgc
     6241 cgcgtgccac atcactttct tcggtacgca gccgacgttc acgcaggtgc cgcccagctc
     6301 tttggcttca atcagcgcgc atttctggcc gtacatagcc gcgcggttga tggaggcgat
     6361 accgccgctg ccgccgccga tggcgatgta atcatagtgt ttagtcatga cagagtgtcc
     6421 ttatcgttga ttaccgcgat tgtagcgcga gacgtaatag gtgccagcaa tggctgcaat
     6481 tactccggca cgatccagct tacggtggcg tgcccggtgc ctgccggaac cagtttgctg
     6541 tgcagccacg gcagcacgtt attcatctgc tgttccagct tccacggcgg gttaatcaca
     6601 atcatgccgg aagcggtcat gccacggcga tcgctgtctg gcagtaccgc cagttcaatt
     6661 tgcagaattt tgcgaatgcc ggtcgcttcc aggtcgtgga tcatgcgctt aatttgctgg
     6721 cgcaacacca ctggatacca cagtgcgtaa gtgccagtgg cgaaacgttt atagccttct
     6781 gctatcccgc tgaccaccgc ctgatagtcg gttttcattt catacggcgg gtcgataagg
     6841 attaaaccac ggcgggaaac cggcggcagt ttggccttaa gctgctggaa accatcggct
     6901 ttttcgacgc gcgcacgatt gtctttctga aattcagaac gtagcaacgg gtaatcgctc
     6961 gggtgcagtt cggtcagttg caggctgtcc tgttcacgca gtaactgacg agcaatcaac
     7021 ggcgaaccgg ggtagtaacg caactgaccg ctacggttga agtgttttac cacattgatg
     7081 tacgcctcca gctctgcagg caaatcgtcc tgctgccaga tacgggcgat accttcgaga
     7141 tattcgccgg tacgctcggc atgttcgctg cctaactgat aacgcccggc ccctgcgtgg
     7201 gtatcgagat agagaaacgg tttatctttc tctttcagcg actcgatgat caggctctga
     7261 acggtatgtt taaggacgtc ggcgtggttg ccagcgtgaa agctgtggcg ataactgagc
     7321 atgggtaaag gtgttccggt aagtaaaaat cggcccgtat tcggggcgca caaaagccta
     7381 tcaggacagt ataccgaaaa gaggccgccg ccgcgaaagc gtaacgtttc tcattgaaat
     7441 tcactacact taaccccatg ctacaaacat tatgtaaagc gcctgttgag cgcttcctta
     7501 acctctttaa ccaggactgc gctaatgacg aatccgttac tgactccctt tgaattgcct
     7561 ccgttttcta aaattctccc ggaacatgtc gttccagccg tgactaaggc gctgaacgac
     7621 tgccgcgaaa atgtggagcg cgtagtagcg caaggggcac cgtacacctg ggaaaatctc
     7681 tgccagccgt tggcggaagt ggacgatgtg ctggggcgta tcttctcccc ggtcagccac
     7741 ctgaactcgg tgaaaaatag cccggaactg cgtgaagcgt acgaacaaac cctgccgctg
     7801 ctgtcagaat acagcacctg ggtaggacaa catgaagggc tgtataaagc atatcgcgac
     7861 ctgcgcgatg gcgatcatta cgccacgctg aacacggcgc agaagaaagc ggttgataac
     7921 gcactgcgcg actttgaact ctctggcatc ggtctgccga aagagaaaca gcagcgttat
     7981 ggtgagattg ccactcgtct ttccgagctg ggcaaccagt acagcaacaa cgtcctcgat
     8041 gcgacaatgg gctggaccaa actcgttacc gacgaagcgg agctggcggg gatgccagaa
     8101 agcgcgctgg ctgcggcaaa agcccaggct gaagcgaaag aacttgaagg ctacctgctg
     8161 acgctggata tcccaagcta cctgccggta atgacctact gcgacaacca ggctctgcgc
     8221 gaagagatgt atcgcgctta cagcacccgc gcctccgatc aaggtccgaa cgccggtaag
     8281 tgggacaaca gcaaggtgat ggaagagatc ctcgcgctgc gtcacgaact ggcgcaactg
     8341 ctgggctttg aaaactacgc ctttaaatcc cttgctacta aaatggcaga aaacccgcag
     8401 caggtgctgg atttcttaac cgatctggca aaacgcgcgc gtccacaagg cgaaaaagag
     8461 ctggcgcaac tgcgtgcctt cgctaaagcc gaatttggcg tggatgagtt gcagccgtgg
     8521 gatatcgcgt actacagcga aaaacaaaaa cagcacctct acagcatcag tgacgaacag
     8581 ctgcgtccgt acttcccgga aaacaaagcg gttaacggct tgtttgaagt ggtgaaacgt
     8641 atttacggca tcaccgctaa agagcgtaaa gatgttgatg tctggcatcc ggatgtacgt
     8701 ttcttcgaac tgtatgacga gaacaacgaa ctgcgcggca gcttctacct cgacctgtat
     8761 gcccgtgaaa acaagcgcgg cggggcgtgg atggatgact gtgtaggcca gatgcgtaaa
     8821 gccgacggtt cgctgcaaaa accggtcgcg tatctgacct gtaacttcaa ccgcccggta
     8881 aatggtaaac cggcgctgtt tacccatgac gaagtgatca ccctgttcca cgagttcggt
     8941 cacggcctgc accatatgct gacccgcatt gaaactgctg gtgtatctgg tatcagcggg
     9001 gtgccgtggg atgcggtcga actgccgagc cagtttatgg aaaactggtg ctgggagcct
     9061 gaggcgctgg catttatctc cggtcactat gaaaccggcg aaccgctgcc gaaggagctg
     9121 ctggataaaa tgctggcggc taagaactac caggcggcgc tgtttattct gcgccagctg
     9181 gagttcggcc tgtttgattt ccgcctccat gccgagttcc gcccggatca gggagcgaaa
     9241 atcctcgaaa ctctggcaga aatcaagaaa ctggttgccg tagtaccgtc tccatcctgg
     9301 ggccgtttcc cgcacgcttt cagccatatt ttcgccggtg gttatgccgc aggttactac
     9361 agctacctgt gggccgacgt gctggcggca gatgccttct cgcgctttga ggaagagggc
     9421 attttcaacc gtgaaaccgg acagtcgttc ctcgacaaca ttctgagccg tggcggttca
     9481 gaagagccga tggatctgtt caaacgcttc cgtggtcgtg aaccgcagct ggatgcgatg
     9541 ctggagcatt acggcattaa gggctgatca ttcagtgaaa atctgcttaa ttgatgaaac
     9601 aggcaccgga gacggtgcct tatctgttct ggcggcccgc tgggggctgg agcacgatga
     9661 agacaacctg atggcgctgg tattaacgcc ggagcatctg gaactgcgca agcgtgatga
     9721 gccaaaactt ggcggcatct tcgttgattt tgttggcgga gcgatggcgc accgacgcaa
     9781 attcggtggt ggtcgcggtg aggcggtggc gaaagcggtg ggcattaaag gcgattattt
     9841 gccggatgtc gtcgatgcta ccgcagggct ggggcgcgat gcctttgtgc tggcttcagt
     9901 cggctgccgc gtgcggatgc tggagcgtaa tccggtggtt gctgcattgc tcgacgacgg
     9961 cctggcgcgt ggttatgcgg atgcggaaat cggcggctgg ttgcaggagc ggttgcagtt
    10021 aattcacgcc tccagcctga cggcgctgac cgatattacc ccacgcccgc aggtggtcta
    10081 tctcgatccg atgttcccgc ataagcagaa aagcgctctg gtgaaaaaag agatgcgtgt
    10141 ctttcagtcg ctggtggggc cggatcttga tgccgatgga ttactagagc ctgcacgctt
    10201 gctggcgacc aaacgcgtgg tggtaaagcg cccggactac gcgccgccgc tggcgaatgt
    10261 cgccacgcca aacgcggtag tgaccaaagg gcatcgcttt gatatttatg ccggaacgcc
    10321 ggtgtaaaag taaagcaggg tagcgttatc gctaccctgc tttctgcaag aataattaat
    10381 ggctttcggg cgtcgcaatc attcgtttca gccacggcac catcagcagc atcaccaccg
    10441 caacgcccag cgtaaccagg ccaatcttac caaacacgtt ggtatagacg ggcaacgtct
    10501 caagcggatc ggtaatgttg tctggtactg cggtaaatgt tgccacatag ccgcccagca
    10561 agaacgcggc agcctgcgtc aggaaccaca tcccgagaat aaagcccatc aaatgctgcg
    10621 gcaccagggc agcaatcatc gctagaccca gggcgctaat aaacaattca cccaggctct
    10681 ggaataagta aaccagcaca ataaaccacg gcgatgtcag tccttgggca tccgcaaacc
    10741 acattcccgc agctgcagcc gtcaaaaagc ccagtgagca cataaacatg ccgagagtaa
    10801 atttcatcgg catagagagg tctttgcctt tgttacccag atgcgtgtaa atgcctgcca
    10861 gtattgggct ggcgagtacc acccagaacg ggttaagcgc ctggaagctg accgggttga
    10921 tggaaaagcc gagaatttca tgatgcacgt tgttgatggc aaagaagttc agcgacgttg
    10981 gcatctgggc gtagagaatg taaaacacca ccgcttcgag catcaggaca aaggcgacaa
    11041 acattttatt gcgcccggtt ttatccagct tgaatgcctg acgaaagaaa atgatggtga
    11101 cgacgataga gagaacaatc agcaccagat tggcgacttc tacgttgtgc atcagccatg
    11161 cacagacgaa gatcatcacc acgctgccaa gtaacacgta caacagtttg ctgaagctca
    11221 tcggccggaa gtcgggttca gaaccaatgt ctttcaccat tccgcgacag gcgatgtaaa
    11281 ccaatagcgc gataattaac cccgcaccgc acaggttgta ggtgactgaa taaccgaatc
    11341 tatcagcgat cacgggggcc agcgataacg ctatcaacga gccgatgttg atcgacatat
    11401 agaacagggt aaatgcgcca tcaagccgcg gatctttcgg cggatagcac ttcgaaagca
    11461 agctggctgg gttagcttta aacaggccgt taccgacagc gatagtcccc agggcgatga
    11521 aaatcaggtc aggcttaagt agcgacaggc cggtcatgaa gtagccaatc gccagcacaa
    11581 gtgctccgag aacaatggtg cgtttggtcc ccagcaggtg gtcgccgaca tagccgccaa
    11641 tggaaatgag gccatagacc agcgcagcaa aagcaccaaa agtgacaaaa gcctgttctt
    11701 gcgagaatcc aagctgctta acgaagaaaa ccgccagtac gccctgcacg ccgtagtagc
    11761 cgaatcgctc ccataactcg acaaaaaaga tcatgaaaaa tgggcgaggt tgctgcagca
    11821 tccccatggg tgttgttgta ttcatattta ttaaccttcc aataccatcc tgaaaagtat
    11881 gaacgcgtaa gggataacgc gcgaaaaagt tttcatctgt cagagattct taatattgat
    11941 gcggtgaata gattaataca ctattacaac agaaaataac cagctgatca cactaatgat
    12001 ggaaaatatg atggtgttaa atgttgtatt ggcaggggat tttgattgcg tacgcagacc
    12061 gtaggccaga taaggtgttt acgctgcaag gtaacaaggc gcgccaaaaa gcaaaacgcc
    12121 cgcatatgcg ggcgttcagg aacacgtcgt agagggaaga ttattcttct tcgtcgcgca
    12181 gcggaacaat cagcatatca acgtgaacgg tgttgatcag ctgacgtgcg gaagacatca
    12241 gtttgctcca gaagtcctgg tggtgaccac aaaccaccag atccatatcg tatttcttga
    12301 ttgcatcgac cagaacctgg cccagatcgc cgctgccgct cagggtttca gtgattgggt
    12361 agcctgcatt agtggaaagc tcggtcagcg catgatgtgt ctcttcagag atgcgtttct
    12421 gcatatcacc cagattcaca tcaataagcc cggtgtatag gtcagagtag tttacatcta
    12481 cgtggatcag agaaactttc gcattgtagg ggcgagccat agagactgct ttctctacca
    12541 gaactttgct ttccggggag agatcgaccg cgatgagaat gtgtttataa gccatagtgt
    12601 tactccttcc ataaagttgt cgatgactgg ccagctagcg tttcttgtgc cgttacgtca
    12661 cccgcgtcct gcgaactacg tgcgcggggc taacccagcc gtaaagattc agtgttaaga
    12721 ccatccttac cttatagcga cccggatgat ccgtcaatcc gccttgctta cccgttagat
    12781 aaacaaattt tcgacatgaa tatattgata gtggttaacc ttctggaaaa aaaacaacct
    12841 gatctcctac actatctata gagccgctcg gatgttggtc accacaaaag cggtttctcc
    12901 aggacctggt gttaactgat tggcagcgta tcgggagcgg tagccccggg gtggaactcc
    12961 gcgggcaggt cgccggggag gaggtatgat aagcaccgtc gcattatttt gggctttatg
    13021 tgtcgtttgc attgttaaca tggcgcgcta tttctcatca ctacgcgcgt tgttagtggt
    13081 actgcgtaac tgcgatccat tgctctatca atatgttgat ggagggggct tttttacctc
    13141 acatggccaa cccaacaaac aggtgcgtct cgtttggtat atctatgccc aacgttatcg
    13201 cgatcatcac gatgatgagt ttattcgccg ctgtgagcgg gtgcgtcggc agtttattct
    13261 gaccagcgca ttgtgtggtc tggtggtggt cagtctgatt gcattgatga tttggcatta
    13321 agcacttcag caaaaataac gcataaaaaa agcgggccag taaactgacc cgctcgtttt
    13381 ggtgcgtgcg attacaggaa ctgcaaggag agccagtaaa gcccgccgga aagcagtacc
    13441 gcagccggaa gggtaaacac ccaggccatc agaatactgg tcacggtttt acgctgtaag
    13501 ccgccgccat ctaccaccat cgtccccgcg acagaagagg agagtacgtg ggtagtggaa
    13561 accggcatcc cggtataact cgccaggccg atagacactg ccgcagtcat ctgggcagac
    13621 atcccctgag cgtaggtcat gcctttctta ccgattttct caccgatagt cgttgccaca
    13681 cgacgccagc caatcatcgt accgatacct aacgccagcg cgaccgccat gatgatccac
    13741 accggcgcat actcgatggt gctaagcata tcggatttca gtttcttcaa caggcgctga
    13801 tcgtcagcac tcacgccagg catcttcacc actttgtcga tagtgtcaga aacgcacagc
    13861 ataatgcggc gcatctggct acgttgatca agcgacagct tgtcgtagct ttccacatcg
    13921 gtagtcaaca tgcctttcag gcggttgagc gcgttaatgg tattcgacgg atggcaatgg
    13981 aactccgcag gttgcgttgc gccagcttcc ggagccggta ctaactgatc agcaccggta
    14041 gcctgtttca gcagcgcagg gtgctgctca aagtaagctt ccacgttgtt gatggcatca
    14101 cgggtacggg tgatttcgta gccagtggca ttcatgttca ccacgaagcc tgctggtgcg
    14161 acgccaatca ataccaacat aaccagacca atgcctttct gaccatcgtt cgcgccgtgc
    14221 gaaaacgcca cgccgatagc ggaaaggatc agcgcaatac gcgtccagaa cggcggcttt
    14281 ttcttgccgt ctttcttttc acgctccgct ggggtcaggt ggatacgggc gcgtttcttg
    14341 gtgccgctcc agtagcgacg cagcaagaaa atcagaccgc cagcaaacac caggccgaca
    14401 ataggggaaa cgatcagaga accgaaaata cttaatactt tcgggatatt gagtgcatcc
    14461 accactgacg tcccggtcat caacgcattg gttaaaccaa tcccgatgat cgcgccaatc
    14521 agcgtatgag agctggatgc aggtaaacca aagtaccagg tacccaggtt ccagataatc
    14581 gccgccagca acatagagaa caccatggca aggccatgag acgatcccat attaagcagc
    14641 agatccgtcg gcagcatatg cacaatggca taggcaacac tcagaccacc cagcaaaaca
    14701 cccaaaaagt tgaacaccgc cgccataacc acggcgagct gagaacgcat cgcgcgggta
    14761 tagataacgg ttgccacggc gttggctgtg tcatggaaac cattgatggc ttcgtagaac
    14821 agcacaaaag ccagtgcaag caataataac agcccggtat gcaaatccag gccagcaaac
    14881 aaatgtagca taggacgtta cgccattttg aggacatgaa cgcggcgcat tatcagtgac
    14941 tttcgcggcg cgggcaaagt gaaatataga ctttttttga tttgcctcct gtatggattt
    15001 cactcaaaaa ataattatct tatataattc aggtaaatac ttccttttag taatattgat
    15061 gctggtgcga ccactgagga atctttacaa ttcacgcccg ttttttctta gaggagcgca
    15121 acgtggaaag gtttgatgcc attattatag gcgctggtgc ggcgggtatg ttctgttctg
    15181 cgctggcagg tcaggcagga cgccgggttc tgctgatcga taatggtaaa aaaccagggc
    15241 gcaaaatcct catgtctggc ggtgggcgct gcaactttac caatctttat gtcgaaccgg
    15301 gcgcttatct gagccagaat ccgcattttt gtaagtctgc actcgcgcgt tttacccagt
    15361 gggatttcat tgatctggtc aataaacacg gcatcgcctg gcacgagaaa acgttagggc
    15421 aactcttctg cgatgactcc gctcagcaga ttgtcgacat gctggtggat gagtgcgaga
    15481 agggcaatgt gaccttcaga ttgcgtagcg aagtgctgag cgtggagaag gatgatacag
    15541 gcttcacgct tgaactgaac ggcatgactg tcggttgcga aaagctagtc atcgcgaccg
    15601 gtggactgtc aatgccgggg ctgggcgcgt cgccgtttgg ttataagatt gccgaacaat
    15661 ttggcctcaa cgtgctgccg acccgcgcgg gtctggtgcc attcactctg cataaaccgt
    15721 tgctcgaaga gttacaggtg ctggcaggcg tggcggtgcc ttccgtgatt accgctgaaa
    15781 acggcaccgt tttccgtgag aacttactct tcacccaccg cggcttatct ggaccggcgg
    15841 tgttgcaaat ttccagctac tggcaaccgg gtgaatttgt cagtatcaat ctgctaccgg
    15901 atgtggacct cgaaaccttc ctgaatgagc agcgtaatgc acatccgaat cagagcctga
    15961 aaaacacact ggcggttcat ctaccgaagc ggttggttga acgtttacaa caactcgggc
    16021 aaatcccgga tgtttcgcta aaacagctca acgtgcgtga ccaacaggca ctgattagca
    16081 cattgaccga ctggcgcgta caacccaacg gcactgaagg ctatcgcact gccgaagtga
    16141 cgctcggcgg cgtggacacc aacgaactct cttcacggac gatggaagcg cgcaaagtgc
    16201 ctgggctgta cttcatcggc gaagtgatgg acgtcaccgg ttggctgggg ggctataact
    16261 tccagtgggc gtggtcgagt gcgtgggctt gtgcgcagga tttgattgca gcgaagtcgt
    16321 cctgagctat ttcctaaaga tgaaaattat tagagtcgca ttaaaaatga gctaattttg
    16381 atagtggtta tcttgtgatt attttctaat gagcctgtga actgagaccc tccatgctta
    16441 atataaggtg gagggaaagg tgattaaaac tcccccagtg gccagatctt tataaaatat
    16501 gaacaggttg aaaaaatcag caacttagct gtttatttat acttaaccac tatgcattaa
    16561 tgctgcgtta tttcatgata cctaagaaaa accagcgtta cgcaaataat caacgctggt
    16621 tttatccggt acgttgcaat tattttttag cagaacccgc ttctaatatt gaaacgattg
    16681 agaacaacgt aaagcatacc gctcccaaac cgaccagaac ccgcgaagga ataaaatatc
    16741 ccatatcggt ctgcgccatt tccgcaagaa acgatgccag gaaaaggcaa aacaggcagg
    16801 tgaagacggg aatcatcggt attctgttgg ctaacgaaca ggtacgtctc cagaccagtg
    16861 ccagtagcca gacttttgag aatatgctgt aacagatcat tccgaggcaa atgaggataa
    16921 tgccgggagc cagtcgggcg cttgcatcgg agctgactaa cacgtatatg ccctgcagca
    16981 ccgtgattga gccgagaata ataacccaat aacaccacag ccagtgttct ttggatgaga
    17041 acgtattacg tgtttgatgg acgattgtgg caacaaggcc aattagacag gcacagattg
    17101 cggttaaccc aagcaatacg tgaccggcga cataatgcgg agttatgtcg gcactacgta
    17161 acagcgtaat agaccaaatg aaaccgagca gggtgagtaa cacgggaacg gcaatgaggc
    17221 aattaccgat taatgacgaa taagcctgta ccggtattcc atcgctcttg ctccctgctg
    17281 catttttggg aatgagcaga aagtgaccgg atgacgctgc cactgtcgat acacaggcgg
    17341 caatcatacc aacgccaaaa ataacatggc cggcaacaaa ctcgtctgcc atcacatcat
    17401 tgcctgctaa cagtgcccaa ccccatatca tggtgataat tgaccccgca tagccaataa
    17461 tggggaacaa tgtattgtaa aacgtattaa cgccgcgcgt gagctgcgaa atgataataa
    17521 atgcggtagt gaataatgcc aggcatatgg ctgccagaga aatcagcaca tgacccgcga
    17581 cgaaatactc actttgccca gaactttcca gcacaaagcc acctaacgcg atacaaatta
    17641 agcccataat taaagggatt aatttgaaaa gccacccgat atatatgttc actttaaact
    17701 cctcaggtat ccctgctaat attgaaatgt gcattaatga taatttcagc ctgttaatat
    17761 aatgacgaga tatcattaaa gacaaatgca tagcataact atagcgggat ttactttaca
    17821 gagcgctact tttcagcgtt caaaaggcca tggtaaataa atatttatcc taagattaat
    17881 tcttattctg ttgtttttta caattatctt ccagggaggg attctttttc attatttggt
    17941 aaaagaccgg atggt
//
LOCUS       DACBVI010000094        17595 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.094, whole genome shotgun sequence.
ACCESSION   DACBVI010000094 DACBVI010000000
VERSION     DACBVI010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.094"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(375..1571)
                     /gene="hemY"
                     /locus_tag="IU325_15155"
     CDS             complement(375..1571)
                     /gene="hemY"
                     /locus_tag="IU325_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HAP5810345.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADDGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1574..2809)
                     /gene="hemX"
                     /locus_tag="IU325_15160"
     CDS             complement(1574..2809)
                     /gene="hemX"
                     /locus_tag="IU325_15160"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAP5810346.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQQETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPAPQADTPAAAPQGE"
     gene            complement(2831..3571)
                     /gene="hemD"
                     /locus_tag="IU325_15165"
     CDS             complement(2831..3571)
                     /gene="hemD"
                     /locus_tag="IU325_15165"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HAP5810347.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKIADNADNDALLRALQ"
     gene            complement(3568..4509)
                     /gene="hemC"
                     /locus_tag="IU325_15170"
     CDS             complement(3568..4509)
                     /gene="hemC"
                     /locus_tag="IU325_15170"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HAP5810348.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4896..7442
                     /gene="cyaA"
                     /locus_tag="IU325_15175"
     CDS             4896..7442
                     /gene="cyaA"
                     /locus_tag="IU325_15175"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HAP5810349.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRREVLTQLVKEWEWDDARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7482..7802)
                     /gene="cyaY"
                     /locus_tag="IU325_15180"
     CDS             complement(7482..7802)
                     /gene="cyaY"
                     /locus_tag="IU325_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HAP5810350.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7850..8110)
                     /locus_tag="IU325_15185"
     CDS             complement(7850..8110)
                     /locus_tag="IU325_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810351.1"
                     /translation="MIGTSRQDGFMHYRNVVCLLACSLFLSSAWGCRLDEPEHNIYQK
                     QGKGVVYLRPYEKTNLSLYRDIIIPLTDDSCQNAEDEQTLKP"
     gene            8269..8472
                     /locus_tag="IU325_15190"
     CDS             8269..8472
                     /locus_tag="IU325_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810352.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8509..9333
                     /gene="dapF"
                     /locus_tag="IU325_15195"
     CDS             8509..9333
                     /gene="dapF"
                     /locus_tag="IU325_15195"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP5810353.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTNDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9330..10037
                     /locus_tag="IU325_15200"
     CDS             9330..10037
                     /locus_tag="IU325_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HAP5810354.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            10034..10930
                     /gene="xerC"
                     /locus_tag="IU325_15205"
     CDS             10034..10930
                     /gene="xerC"
                     /locus_tag="IU325_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP5810355.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQYDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            10930..11646
                     /gene="yigB"
                     /locus_tag="IU325_15210"
     CDS             10930..11646
                     /gene="yigB"
                     /locus_tag="IU325_15210"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HAP5810356.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            11730..13892
                     /gene="uvrD"
                     /locus_tag="IU325_15215"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             11730..13892
                     /gene="uvrD"
                     /locus_tag="IU325_15215"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HAP5810357.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTTIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(13937..14839)
                     /locus_tag="IU325_15220"
     CDS             complement(13937..14839)
                     /locus_tag="IU325_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HAP5810358.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAQTLGIDSVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(14922..15685)
                     /locus_tag="IU325_15225"
                     /pseudo
     CDS             complement(14922..15685)
                     /locus_tag="IU325_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16055..17005
                     /gene="corA"
                     /locus_tag="IU325_15230"
     CDS             16055..17005
                     /gene="corA"
                     /locus_tag="IU325_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HAP5810359.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(17046..17537)
                     /locus_tag="IU325_15235"
     CDS             complement(17046..17537)
                     /locus_tag="IU325_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HAP5810360.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQMLYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFKK
                     DNF"
BASE COUNT         4067 a         4559 c         4652 g         4317 t
ORIGIN      
        1 tgcgcaggag aaatattcac tcaggaagtt attactcagg aagcaaagag gattacagaa
       61 ttatctcata aaaagtgtta agggatgtta tttcccggtt ctctgtggca taataaacga
      121 gtagatgctc attccatctc ttatgttcgc cttagtgcct cataaactcc ggaatgacgc
      181 agagccgttt acggtgctta tcgtccactg acagatgtcg cttatgcctc atcagacacc
      241 atggacacaa cgttgagtga agcacccact tgttgtcata cagacctgtt ttaacgcctg
      301 ctccgtaata agagcaggcg tttttttatg tatcagaaag gtcccggagg tgcttgcctc
      361 cgggtgagaa agaactactg tggcgggttg ttctgcaacg ttaacatcaa accgtcgcgg
      421 cgcatcgctg cagcttcttc tggtttgtgc agtctgtcca gcgcgtcggc gagccatgcg
      481 taatcgtagg cgtccggacg ttgtttcagc gccgcgcgga aggcgagcga tgcttcctgc
      541 cattcaccgt gcttcatcag tgactgaccc aatgtgctcc acaacagcgg gcgatcaccg
      601 acgtttttga tttgctggcg tagcactttt tccagctgtt ccgggttatt ggttttcaga
      661 cgcgggatcg gcagcagcag acgatcatca tactggcgtt tcaggccgtc gatgataatt
      721 tgctgagcag tatcatgatc gtcacattca ataagatgtt ccgccattgc cacctgtaac
      781 gccacctgat gacgcgtttt ccggctttgg tttttccacc agtttcgcaa accttcgcta
      841 ccgtcatcgg cgcgcgcctg atccatcagg ccaatccatg cctgttgttc cagcattgca
      901 cgatgttctt catcgccaac gtgggctttc gccattgatg ggatgatatc cagcagcgaa
      961 ctccatgcac ctgtgcggat atacgcctgt tccgccagac gtaatacttc cggatggcgt
     1021 ggcgtaactt ccagcagctt atccacgccg tggcgtgcag catggttttc attacgggcc
     1081 agttgaagac gtacacgggt gatttctacc ggaatggtgt cgttgccggc cagctccgct
     1141 gcacgttcca gatgttggtt ggcgcgtgct tcatcaccac gttgttgcgc ggcttcggca
     1201 gccagtagat agttcaccac cggttgttca gcgtgatcgg catttttcgc catcagcttt
     1261 tcaacttgct gataatcgcc ttccgctagt ttcagcagcg cctgctcggt ctgcttacgg
     1321 gcacggcgac gcttacgtcc gacaaaccac ccacgggtgt gtgcgcctgt gcggaagatc
     1381 cgccgcagta gccactcaat ggcaaacagc actaccatcg ccagaatcaa aataatcgcc
     1441 aggccagtga cgctggtttc gatattgtag ttgtcggtct ggatcagcac gtaaccctga
     1501 tgaccggcaa tcatcgggcc aaccactatc cccgcaatca gcaaaacaaa gagcaataac
     1561 acttttagca tggttattct ccttgcggcg cggctgccgg agtatcagct tgcggtgcag
     1621 gtgcaggtgc aggtgcaggt gcaggtgcag gtgcaggtgc aggtgcaggt gcaggtgcag
     1681 gtttagcttc cgttgtcccc gctgccggtt gtgccagcag gttacgcacg cgagtctgca
     1741 tcagtttttc cagcatcgcc tggctttgca gggtttccgg aagatccatc gagatatttt
     1801 gctggcttaa ctggtccacc tcgtcgagga acgctttggt ggtggcatca tcagtatcgt
     1861 agtaagcacg tacccaggtg gagacgttct ccagcgcctg gcgataagtc tcttgctggt
     1921 gacgcggtac agcttgtgct gcgaccagca ggcgagagcg aatattttcg cgcagataga
     1981 tatcctgatt tggcgctaac agcggtacgg cggtgtcatc acgacggcga atcgtaatga
     2041 agttgtccat aaaattctgc cagctttttt gtagattgat acgccattcg ctgatggaac
     2101 tggaaagctc ttcaccgtcg gaatccatcg gcgaaccatc gctgtcatta tcggccagac
     2161 gcaggttatc tacctgattt gaaagctgat taagcttaag gatgatgccg tcataatcca
     2221 cctgcgatac cgcagaaagg ctggcgatat catcggtaat tgcccgacga acggtaatca
     2281 gactcgggtc attcatatcc gccaggctgg catctgcact tttcagcaac gctgcagcgg
     2341 tcgtgacgtc ttgatcgctc cacagcttcc gtcctgcgag tttcaccaga aaatcggcct
     2401 gagccagcag ccaggtttta gcatcgctgc cggaaatggt ggcgaccttt tgttggactt
     2461 catccaactg ttttgccagc gtttcttgct gacgattcgc ctgttcaagc tgcgtagcct
     2521 gttgcttgat aatgccttcc agctcggctt tttggctctc ctgggctttt tgcaatgccg
     2581 tcagttggtt agccagggca tcgctggtgg cggtctgatt gacggcctgt tgtttacccc
     2641 agccatacaa accgacgccc gccgccagag caatagcgat agccaccgcg ctgagaatca
     2701 atgcggtatt gttcttactc tttttttctg ttgcgacagg ttgtgacgtg gtgtccacgg
     2761 cctccctggt ctcttcaacc acggcggagg ttttttcttg ttccgtcatt atggcttcct
     2821 gttatgagag ttattgtaat gcccgtaaaa gcgcatcgtt gtcagcgtta tcggcgatct
     2881 taatgtcttg ccagcccagt tcccgggcga gtttcgccaa acgctcactg acgaccaata
     2941 gtcgacagtg tagtaaccag tgctcacgat accattgtgg gatcagcgac cagagttgct
     3001 gcaacatttc accgctggta acaacgaccg tcgtcacctc gcgggattgc cagcgcatcg
     3061 cttcttctgc accatcgtaa tggattgcgc atcgttgata acattcacaa aaagtgacct
     3121 cagcaccgcg cgccgtcagg gtatccccaa ttagctcacg accgccattg ccacgtaata
     3181 tcagcgcacg tttgcccgca atattttgta attcaggtaa ttgtagcaag acttcgctga
     3241 tttcccgatc ctgcgggtag agaatcttct gtccgcttac agtatgtagc gccagtgcgg
     3301 tggtgcgtcc aatggcgaaa taatcaggta gtcggggcca tttacgatct tgctgatgca
     3361 gctgtgattg ggcaaaagca accgcgtgtt gcgagagggc aaacaacaga tcgctctccc
     3421 ccagcgctgc aagttgatca gcaagttgcg gtaattgtcg acccggagaa aactcaatca
     3481 gcggaaaatg ccaggccacc tgccccagtg tgcgcagacg gctcactaac tcttctccag
     3541 cgggagacgg gcgggtgacc aggatactca tgccggggcg tctccgttat agacttcagc
     3601 aaggatctcg cgcgcgccgt tattcagtag ctcttctgcc agcgaaatcc ccatttgttc
     3661 ggcatcttgc ggcgcaccgc ggcgttcacc gcgaataatc tgcgaaccgt ccggcgcgcc
     3721 gaccagcgca cgcagccaga tttcgccatc aataagctcg gcgtagctac caattggtac
     3781 ctgacatccg ccttcgagac gggtattcat ggcgcgttct gcggtaacgc gcagtgcagt
     3841 ttcgtggtga ttcagcgcgg caagcagctc gcgagtgcgt gtatcatcaa ggcggcattc
     3901 aataccgacc gcaccttgtc ctaccgccgg aagagaaatc tcgggtggca acgcggcgcg
     3961 aatgcgtgac tccagaccta aacgttttag tccggctacg gcaagaatga tggcatcgta
     4021 ttcgccgtta tccagtttgc tcaggcgagt gccgacgttg ccgcgcaggg agcggataat
     4081 cagatccggg cggcgttcag ccagttggca ctggcgacgt aaactggacg tcccgacgat
     4141 actgcctgcc ggtaacgcat ccagactgtc atagttattg gacacaaagg catcgcgagg
     4201 atcttcacgc tcacaaatag tgaccagtcc cagaccttgc gggaattcaa ccggcacatc
     4261 tttcattgaa tgtacggcga tatcggcgcg attttcgagg agcgcgactt ccagctcttt
     4321 tacaaataag ccttttccgc ctactttcgc cagcggcgta tcaagaatca catcgccgcg
     4381 cgtcaccatc ggtaccagtt caacgaccag gcccggatgg ctcgccatca acttgtcttt
     4441 gacatagtgt gcctgccaga gtgcaagtgg gctttggcgt gtggcaattc ttaaaacatt
     4501 gtctaacatg cttgttaccg tcattatcat ccgtggccca tcctaacatc cttgccagag
     4561 cgatgtcagt gttgtggtga aacgtagacg cctgcgcaaa ccgtaaaatg aggtctggca
     4621 gtggatcctg acaggcgttt cacgccgtcg taataaggaa tttacagaga ataaacggtg
     4681 ctacacttgt atgtagcgca tctttcttta cggtcaatca gcaaggtgtt aaattgatca
     4741 cgttttagac cattttttcg tcgtgaaact aaaaaaacca ggcgcgaaaa gtggtaacgg
     4801 ttacctttga catacgaaat atcccgaatg ccgcgtgtta ccgttgatgt tggcggaatc
     4861 acagtcatga cgggtagcaa atcaggcgat acgtcttgta cctctatatt gagactctga
     4921 aacagagact ggatgccata aatcaattgc gtgtggatcg cgcgcttgct gctatggggc
     4981 ctgcattcca acaggtctac agtctactgc cgacattgtt gcactatcac catccgctaa
     5041 tgccgggtta ccttgatggt aacgttccca aaggcatttg cctttacacg cctgatgaaa
     5101 ctcaacgcca ctacctgaac gagcttgaac tgtatcgtgg aatgtcagta caggatccgc
     5161 cgaaaggtga gcttccaatt actggtgtat acaccatggg cagcacctcg tccgtagggc
     5221 aaagttgttc ctctgacctg gatatctggg tctgtcatca atcctggctt gatagcgaag
     5281 agcgccagtt actacaacgt aaatgcagcc tgctggaaag ctgggccgcc tcgctgggcg
     5341 tggaagttag cttcttcctg attgatgaaa atcgcttccg tcataatgaa agcggcagcc
     5401 tggggggcga agattgtggc tccacccagc atatactgct gcttgacgaa ttttaccgta
     5461 ccgccgtgcg tctcgctggt aagcgtattc tgtggaatat ggtgccgtgc gacgaagaag
     5521 agcattacga cgactatgtg atgacgcttt acgcgcaggg cgtgctgacg ccaaatgaat
     5581 ggctggatct cggtggctta agctcgcttt ctgctgaaga gtactttggt gccagccttt
     5641 ggcagctcta caagagtatt gattccccat acaaagcggt gctgaaaaca ctgctgctgg
     5701 aagcctattc ctgggaatac ccgaacccac gtctgctggc gaaagatatc aaacagcgtt
     5761 tgcacgacgg cgagattgtg tcgtttggcc tcgatcctta ctgcatgatg ctggagcgtg
     5821 ttactgaata cctgacggcg attgaagatt tcactcgtct ggatttagta cgccgctgct
     5881 tctatttaaa agtgtgcgaa aaactcagcc gtgaacgcgc ctgcgtaggc tggcgtcgtg
     5941 aagtgctaac ccagttagtg aaagagtggg aatgggacga tgctcgtctg gcaatgctcg
     6001 ataaccgcgc taactggaag attgatcagg tacgtgaggc gcacaacgag ttgctcgacg
     6061 cgatgatgca gagctaccgt aatctgattc gctttgcgcg ccgcaataac cttagcgtct
     6121 ccgccagtcc gcaggatatc ggcgtgctga cgcgtaagct gtatgccgcg tttgaagcat
     6181 tgccgggtaa agtgacgctg gtaaacccgc agatttcacc cgatctctcg gaaccgaatc
     6241 tgacctttat ttatgtgccg ccgggccggg ctaaccgttc aggttggtat ctgtataacc
     6301 gcgcgccaaa tattgagtcg atcatcagcc atcagccgct ggaatataac cgttacctga
     6361 ataaactggt ggcgtgggca tggtttaacg gcctgctgac ctcgcgtacc cgcttgtata
     6421 ttaaaggcaa cggcattgtc gatttgccta agttgcagga gatggtcgcc gatgtgtcac
     6481 accatttccc gctgcgcctg cctgcaccga cgccaaaagc gctctacagc ccgtgtgaaa
     6541 ttcgccatct ggcgattatc gttaacctgg aatatgaccc gacagctgcg ttccgcaatc
     6601 aggtggtgca ttttgatttc cgtaagctgg acgtcttcag ctttggcgag aatcaaaatt
     6661 gcctggtagg tagcgtggac ctgctgtacc gcaactcgtg gaacgaagtg cgtacgttgc
     6721 acttcaacgg cgagcagtcg atgatcgaag ccctgaaaac cattctcggt aaaatgcatc
     6781 aggacgccgc accgccagat agcgtggaag tcttctgcta cagccagcat ctgcgcggat
     6841 taatccgtac tcgcgtgcag caactggttt ctgagtgcat tgaactgcgt ctttccagca
     6901 cccgccagga aaccgggcgc ttcaaggcgc tgcgcgtttc tggtcaaacc tggggcttat
     6961 tcttcgagcg cctgaatgta tcggtacaga aactagaaaa tgccatcgag ttttatggcg
     7021 ctatttcgca taacaaactg cacggcctgt cagtgcaggt tgaaaccaat cacgtcaaat
     7081 taccggcggt ggtggacggc tttgccagcg aagggatcat ccagttcttc ttcgaagaaa
     7141 cgcaagacga gaatggcttt aatatctaca ttctcgacga aagcaaccgg gttgaggtgt
     7201 atcaccactg cgaaggcagc aaagaggagc tggtgcgtga cgtcagtcgc ttctactcgt
     7261 catcgcacga ccgttttacc tacggctcaa gcttcatcaa cttcaacctg ccgcagttct
     7321 atcagattgt gaaggttgat ggtcgtgaac aggtgattcc gttccgcacc aaatcgattg
     7381 gcaacatgcc gcctgccaat caggatcacg atacgccgct attacagcag tatttttcgt
     7441 gatgaacgtg ccggaaagcg aggcttatcc ggcatgcaat cttagcggaa actgactgtt
     7501 tcacccgcct gctgcgtcgc cgcctgttcc agcaaatccc agaaggtttc gccgctacga
     7561 tcgcaaatcc actcatcgcc tttcagatca aaatggtagc cgccttgttt ggttgccagc
     7621 catacctggt gcagcggctc ctggcggttg ataatgattt tgctgccgtt ttcaaaggta
     7681 atggtcagta caccgccgtt gatttcacag tcgatatcgc tgtcgccatc ccagtcgtcc
     7741 agacgctctt caatggtcag ccacagttga tcagccaggc gatgaaattc actgtcgttc
     7801 attgttgtat cctgttttta agtgatggcg gcagtatagc ggcatggggt cagggcttca
     7861 aagtttgctc gtcttctgcg ttctggcatg aatcatccgt caacggaata ataatgtctc
     7921 tatatagcga aagattggtc ttttcataag ggcgcaaata caccacgcct tttccctgtt
     7981 tctggtaaat attatgttcc ggctcatcaa gccgacatcc ccatgccgac gataaaaaca
     8041 acgaacaagc caacaaacaa accacattgc gatagtgcat aaagccatcc tggcgcgagg
     8101 tgccgatcac gaaactacca gcaaaacata aatcgccacg agtaagcgtt atactcgcag
     8161 catttcctca cttttcagac ttcataaaga gtcgctaaac gcttgctttt acgccttctc
     8221 ctgcgatgat agaaagcaga aagcgatgaa ctttacaggc aatccataat gaaaaacgtg
     8281 tttaaggcac tcactgtatt acttactctc ttcagcctga cgggctgcgg tctgaaaggt
     8341 ccgctctatt tcccgcctgc agataaaaac gcaccgccgc cgaccaaacc ggtagagacg
     8401 caaacgcaat ccacagtgcc ggataaaaac gaccgcgcca ctggcgatgg tccatcccag
     8461 gtgaattact aaaagtcagt ttctgtaccc gcgtgattgg agtaaatgat gcagttctcg
     8521 aaaatgcatg gccttggcaa cgattttatg gtcgtcgacg cggtaacgca gaatgtcttt
     8581 ttttcaccgg agctgattcg tcgcctggcg gatcggcacc tgggggtagg gtttgaccaa
     8641 ctgctggtgg ttgagccacc gtatgatcct gaactggatt ttcactatcg cattttcaat
     8701 gctgatggca gtgaagtggc gcagtgcggc aacggcgcgc gctgctttgc ccgttttgtg
     8761 cgtctgaaag ggctgaccaa taagcgcgat atccgcgtca gcaccgccaa cggacggatg
     8821 gttctgaccg tcaccaatga tgatctggtc cgcgtcaata tgggcgaacc caacttcgaa
     8881 ccttctgccg tgccatttcg cgctaacaaa gcggaaaaga cctatattat gcgcgccgcc
     8941 gagcagacaa tcttatgcgg cgtggtgtcg atgggaaatc cgcactgcgt gattcaggtc
     9001 gatgatgtcg ataccgcggc ggtagaaacg cttggtcctg ttctggaaag ccacgagcgt
     9061 tttccggagc gcgccaatat cggttttatg caagtggtta agcgcgagca tattcgttta
     9121 cgcgtttatg agcgtggggc aggagaaacc caggcctgcg gcagcggcgc gtgtgcggcg
     9181 gtggcggtag ggatccagca aggtttgctg gccgaagaag tacgcgtgga actccccggc
     9241 ggtcgtcttg atatcgcctg gaaaggtccg ggtcacccgt tatatatgac tggcccggcg
     9301 gtacatgtct acgacggatt tattcatcta tgaagcaacc aggggaagaa ctgcaggaaa
     9361 cactcacgga gcttgatgac cgggcggttg tcgattatct gattaaaaat cctgagtttt
     9421 ttatccgcaa tgcgcgcgca gttgaagcga tacgtgtgcc gcatccggta cgcggcaccg
     9481 tttcgttggt cgaatggcac atggcccgcg cacgtaatca tattcatgtt ctggaagaga
     9541 acatggcgct gttgatggaa caggctatcg ccaacgaagg cctgttttat cgcttactct
     9601 acctgcagcg tagtctcacc gccgccagca gtctcgacga tatgctgatg cgctttcacc
     9661 gctgggcgcg cgatctcggc ctggcaggtg cgagtctgcg cctgtttcca gatcgctggc
     9721 gcttaggtgc gccgtcgaac cacactcatc tggcattaag ccgtcagtct ttcgaaccgc
     9781 tgcgtattca gcgtctgggg caggaacagc actatctggg gccgcttaac ggaccggagc
     9841 ttctggtggt gctaccggaa gcgaaagcgg tgggatcggt ggcgatgtcg atgctgggaa
     9901 gcgatgctga tttgggtgtt gtgctgttta ccagtcgcga tgccagtcac tatcaacaag
     9961 ggcagggaac gcagttactt catgaaattg cgctgatgtt gccggagctt ctggagcgtt
    10021 ggattgaacg cgtatgaccg atttacacac cgatgtagaa cgctacctac gttatctgag
    10081 cgtggagcgc cagctaagcc cgataaccct gcttaactac cagcgtcagc ttgaggcgat
    10141 catcaatttt gccagcgaaa acggcctgca aagctggcag caatatgatg tgacgatggt
    10201 gcgcaatttc gctgtacgca gtcgccgtaa agggctggga gctgcaagtt tggcgttacg
    10261 gctttctgcg ctacgtagct tttttgactg gctggtcagc cagaacgagc tcaaagctaa
    10321 cccggcgaaa ggtgtttcgg caccgaaagc gccgcgtcat ctgccgaaaa acatcgacgt
    10381 cgacgatatg aatcggctgc tggatattga tatcaacgat cccctcgctg tgcgcgatcg
    10441 ggcaatgctg gaagtgatgt acggcgcggg tctgcgtctt tccgagctgg tggggctcga
    10501 tatcaaacac ctcgacctgg agtctggtga agtgtgggtg atgggaaaag gcagcaaaga
    10561 gcgccgcctg ccgattggtc gcaacgctgt ggcgtggatt gagcactggc ttgatttgcg
    10621 cgacctgttt ggaagcgaag acgatgcgct ttttctgtcg aaactgggca agcgtatctc
    10681 cgcgcgtaat gtgcagaaac gctttgccga atggggcata aaacaagggc tgaataatca
    10741 cgttcacccg cataaattac gtcactcgtt cgctacgcat atgctggagt cgagcggcga
    10801 tcttcgtggt gtgcaggagc tgctgggtca tgccaacctc tccaccacgc aaatctatac
    10861 tcatcttgat tttcaacacc ttgcctcggt gtacgatgcg gcgcatccac gcgccaaacg
    10921 ggggaaataa tgcgttttta ccggcctttg gggcgcatct cggcgctcac ctttgacctg
    10981 gatgataccc tttacgataa ccgtccggtg attttgcgca ccgagcgaga ggcgcttacc
    11041 tttgtgcaaa attatcatcc ggcgctgcgc agcttccaga atgaagatct gcaacgcctg
    11101 cgccaggcgg tacgggaagc ggaacccgag atttatcacg acgtgacgcg ctggcgtttt
    11161 cgttcgattg aacaagccat gctcgacgcc gggctgagtg ccgaagaagc cagtgcaggc
    11221 gcacacgcag caatgatcaa ctttgccaaa tggcgcagcc gaatcgatgt tccgcagcaa
    11281 acccacgaca ccttaaaaca gctggcgaag aaatggccgc tggtggcgat caccaacggt
    11341 aacgcccagc cggagctgtt tggtttaggg gattattttg agtttgtgct gcgcgctggc
    11401 ccgcacgggc gctcaaaacc gttcagcgat atgtactttc tggctgcgga aaaactcaac
    11461 gtgccgatcg gcgagatctt acatgttggg gacgatctca ccactgacgt gggtggggca
    11521 attcgcagcg gaatgcaggc ttgttggatc agaccggaaa atggcgatct gatgcaaacc
    11581 tgggacagcc gtttactccc gcatctggaa atttcccggt tggcatctct gacctcgctg
    11641 atataatcag caaatctgta tatataccca gctttttggc ggagggcgtt gcgcttctcc
    11701 gcccaaccta tttttacgcg gcggtgccaa tggacgtttc ttacctgctc gacagcctta
    11761 atgacaaaca gcgcgaagcg gtggccgcgc cacgcagcaa ccttctggtg ctggcgggcg
    11821 cgggcagtgg taagacgcgc gtactggtgc accgtatcgc ctggctgatg agcgtggaaa
    11881 actgctcgcc atattcgatt atggcggtaa cgtttaccaa caaagcggcg gcggagatgc
    11941 gtcatcgtat cgggcagctg atgggcacca gccagggcgg catgtgggtc ggcaccttcc
    12001 acgggctggc gcaccgtctg ctgcgtgcgc accatatgga cgccaatctg ccgcaggatt
    12061 ttcagatcct cgacagcgag gaccagctac gcctgctcaa acgcctaatc aaggcgatga
    12121 acctcgacga gaagcagtgg ccgccgcgcc aggcaatgtg gtacatcaac agccagaaag
    12181 atgaaggcct gcgtccacat catattcaaa gctacggtaa tccggtggag cagacctggc
    12241 agaaggtgta tcaggcgtat caggaagcgt gtgaccgcgc gggcctggtg gacttcgcgg
    12301 aactgctgct acgcgcgcac gagctgtggc ttaacaagcc gcatattctg caacactacc
    12361 gcgaacgttt tactaccatc ctggtagacg aattccagga taccaacaac attcagtacg
    12421 cgtggatccg cctgctggcg ggcgacaccg gcaaagtgat gatcgttggc gatgacgacc
    12481 agtcaatcta cggctggcgt ggggcgcagg tcgagaatat tcagcgtttc ctcaatgatt
    12541 tccccggtgc tgaaactatc cgtctggagc agaactaccg ctctaccagc aatattctga
    12601 gcgccgcgaa cgccctgatt gaaaacaaca acgggcgttt gggtaaaaaa ctgtggaccg
    12661 atggcgcgga tggtgagcct atttccctct attgcgcttt taacgaactc gatgaagcgc
    12721 gttttgtggt taaccgtatc aaaacctggc aggacaacgg cggggcgctt gccgagtgcg
    12781 ccattctcta ccgcagcaac gcccagtcgc gtgtgctgga agaagcactg ttacaggcaa
    12841 gtatgccgta ccgtatttac ggcgggatgc gattcttcga acgccaggaa atcaaagatg
    12901 cgctctcgta tctgcgcctg attgccaacc gtaatgacga cgcggccttt gagcgcgtgg
    12961 tgaatacacc aacgcggggt attggtgacc ggacgctgga cgtggtacgt cagacatcgc
    13021 gcgatcgcca gttaacactc tggcaggcat gtcgtgaact gttgcaggaa aaagccctcg
    13081 ccggacgtgc tgccagtgcc ttacagcggt ttatggaact gatcgacgcc ttagcgcagg
    13141 aaactgccga tatgccgctg catgtacaga ctgaccgggt aattaaagac tccggcctgc
    13201 gcacaatgta cgagcaggag aaaggcgaaa aaggccagac gcgtatcgaa aacttagagg
    13261 aactggtgac ggcgacgcgc cagttcagct acaacgaaga agacgaagat ttaatgccgc
    13321 tgcaggcgtt cctctcccat gcggcactgg aagcgggcga ggggcaggcg gatacctggc
    13381 aggatgcggt gcagttgatg acgctacact cggcgaaagg tctggagttc ccgcaggtgt
    13441 ttatcgtcgg tatggaagag ggcatgttcc caagccagat gtcgctggat gaaggtgggc
    13501 gtctggaaga agaacgccgt ctggcctacg ttggcgtaac ccgcgcgatg cagaaactga
    13561 cgctgaccta cgcggaaacc cgccgtctgt atggcaaaga agtttaccat cgcccgtcgc
    13621 gctttatcgg cgagctgccg gaagagtgtg tggaagaggt gcgcctgcgc gccacggtaa
    13681 gccgcccggt cagccatcaa cggatgggta cgccgatggt cgagaacgac agcggctaca
    13741 agctcggcca gcgcgtacgc cacgctaagt ttggtgaagg caccattgtc aatatggaag
    13801 gcagcggtga gcatagccgt ttgcaggtgg cgttccaggg ccagggtatt aaatggctgg
    13861 tggcggctta tgcccggttg gagacggtgt gacgttgcgg gatgcggtgc gccacaccgc
    13921 attccgcatc agcaccttac ttagcgcact tcttcacatc aatattccag tcagcaccag
    13981 acgcaacctg atgtttctgc gcaaaattat ccatattcag cgccacggaa acattcaata
    14041 agctgttcag atacaccatc ggctggcctt ccggcacatc gccgaagctt gcgacatacg
    14101 gcattttccc tgcgtatttt tgctgggaac cttcggaaat ggtcacacac aacgtgtcgt
    14161 tgagtttgat tcctgcctga ttcagcaact cgtcgctgat attgctccag acgttgccgt
    14221 actgaatatc gagaatcggg atattgccct tcacttcacc ttttgttgct tttgctttct
    14281 gataagaaag ttcgacaact tttgcaggaa gctctggccc cacctgttcg aacgtaatcg
    14341 ctccggaagc cagacgcgca ccagtgtagg catatacatc gcgaccatgg aaggtatatg
    14401 acttttcaga acctttcagg cggttagctt tttcgtcgat ttcccggacg ctatcaatac
    14461 ccagagtttg tgccaccaaa gtgagcgtac cgttatctgg tgaaacgaag tattgaccgt
    14521 ttttggtttt cagcactacc gatttacggt cagtgcctac gcccggatcg accacgctaa
    14581 caaacaccga accttgcggc cagtaactgg cagtctgata caggcgataa gcgccttccc
    14641 agatgttata cggtggaatt tcgtgtgtta aatcaaagat tttaagatta tggtcaacgc
    14701 cgaatgcgac ccctttcatc gccgagacgg caccatcttt caggctaaaa tcagtttgta
    14761 aaatcaaagc gttatccgca taggcggatg atgatcccag acacgttaat aaaaataacg
    14821 ttgcgagcgt ttttttcatt tttgtttccc ttcatttatc tgatgatttt ccagctgtgt
    14881 agccagtccc agtttgtctg gcctcctcga gtgtacaatg cttaaccttt ccgctcgacg
    14941 gaaatcatgg tgacaaaagg atagcgttgc cacgggattg ccccgccttt catatacata
    15001 tgtgaaattg tgccatcaag ataaagcaac tgctcaacgt tcagtttcgc tttggcataa
    15061 caggcaaaat cataaaaatt tgtcgcctgc tggctcaaca aaaacacggc gttcccttgt
    15121 ttattaatcc tacaccgtta cgaattttac gcgaggcaac attcgggtga atgcgtggat
    15181 taattacgcc gttttctaat aacattggcc ctgactgcac cgcaaactga atctctttac
    15241 tggttttaaa ggcatccaga cgaacgatgc cgactttatc tcccgcgaca taaaacacgc
    15301 cgccaggacg aataaagaaa ttcccttcac ctgaagcgag atttaacgcc accttctgct
    15361 gaccgttttc gatgtacaaa ccgagcggcg catagctttc atcatagatg ccgccgttca
    15421 tcgccatctg cacctgaccc tgactattca tatccgccag cagagcatgt aacgttcccc
    15481 acgcttcgcc attggctttt tgccagtaca ttttcacccg ctctgtttga ggattaacgg
    15541 tatacgcctg tacggtcagc gtcggatctg agagtgcgca atcatcagcg gcaacagcaa
    15601 acaagggaag taaggtgagg gcgagaaaaa tccgtttgag attcaaggtg atcattcctt
    15661 taccaatgag tagccgatgc gccattatag gtcctggatg tgggatttct ttatcctgtt
    15721 agcgaccttg acgagtacca aaaagcgcga agttcaacta ttgttctgtg gtgttctgtt
    15781 gcgtgttgac ggcaaaattt tgctggcgta acatgcgcgc acgatcactc taagaggaca
    15841 ttcgccttgg acacacccag tagatactgg ctcactatcc tgtcatccag gatcaactcc
    15901 taaggctatc cctttttgct gatagcctta gcggttgtca gcgacctcaa tttttcccgt
    15961 cgcgctgagt caggctgttt aatggtctga aacccaattt gtttctgtgt gcccaccgaa
    16021 ctgtccgata ttttaagcat tgggagtccc ggtcatgctg agcgcatttc aactggaaaa
    16081 taaccgactg acccggctgg aagtcgaaga gtcacaaccc cttgtaaatg cagtatggat
    16141 tgatcttgtc gaaccggacg acgacgagcg actgcgcgta caatctgaac ttggtcagag
    16201 cctggcaacc cgcccggaac tggaagacat cgaagcatcg gcacgtttct ttgaagacga
    16261 tgacggcctg catattcact ccttcttctt ctttgaagat gcggaagatc acgccggtaa
    16321 ctccactgtg gcatttacca tccgtgatgg tcgtctgttt actctgcgtg agcgtgaact
    16381 gcctgcattc cgtctgtatc gtatgcgtgc ccgtagccag tcgatggtag acggtaatgc
    16441 ctacgagctg ctgctggatc tgttcgaaac caaaatcgaa cagttggcag atgaaattga
    16501 aaatatctac agcgacctgg agcagttgag ccgggtaatt atggaagggc atcagggcga
    16561 tgagtacgac gaggcgctct ccacgctggc ggaactggaa gatatcggct ggaaagttcg
    16621 cctgtgtctg atggataccc agcgtgcgct caacttcctg gtgcgtaagg cgcgtttacc
    16681 gggcgggcaa ctagagcagg cgcgtgaaat cctgcgagat atcgaatccc tgctgccgca
    16741 taacgaatct ctgttccaga aggtgaactt cctgatgcag gcggcgatgg gctttatcaa
    16801 catcgagcag aaccgcatca tcaaaatctt ctcggtggta tccgtcgtgt ttctgccgcc
    16861 aacactggtt gcttccagct acgggatgaa ctttgagttt atgccagaac tgaagtggag
    16921 tttcggctac ccaggcgcga ttatctttat gatcctcgcg ggcctggcac cgtatctgta
    16981 ctttaagcgg aagaactggt tgtaatatga gtgccggata aagctatttt ttatccggct
    17041 tatttctaaa aattgtcttt tttaaagtca tgcggattga aaactcgctc tattttattt
    17101 tcttgtgtta attgatactc agtaaaatcg ttaataccaa tatgagtgtt acgataaggg
    17161 tattcattaa taaccattaa gtaatttttc acgatgcaat tatcattgct cttaattgtc
    17221 gggaaacagt agagatcttc atacggccct tcgatatatt ttttatagtc agtgaagcca
    17281 ttaccgatat caatgacata ccaggtatgc caactacctt ctggtagggg atgttgattt
    17341 ttaatttcat tctgatgctt cagataccaa tttattttat cgctgtcagt ccatggcaga
    17401 tgatcaacga ccagaaagac tgaattgcca gcatcagagt atgtatacaa catctgtaca
    17461 ggacgcaatg tccataaatt atagatgcag catagaagaa ataaacataa tataattttc
    17521 tggagtttac ttttcataga taatttttga ttctttcgat agtgttaaaa ttggtgaaaa
    17581 aaggtttgaa agcaa
//
LOCUS       DACBVI010000095        17397 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.095, whole genome shotgun sequence.
ACCESSION   DACBVI010000095 DACBVI010000000
VERSION     DACBVI010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.095"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            94..459
                     /locus_tag="IU325_15240"
     CDS             94..459
                     /locus_tag="IU325_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HAP5810361.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISA
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            609..1127
                     /locus_tag="IU325_15245"
     CDS             609..1127
                     /locus_tag="IU325_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4154 domain-containing protein"
                     /protein_id="HAP5810362.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSHFSNALQENGTTSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            1117..2343
                     /gene="dgcN"
                     /locus_tag="IU325_15250"
     CDS             1117..2343
                     /gene="dgcN"
                     /locus_tag="IU325_15250"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HAP5810363.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGSAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIVQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTACILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEVAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            2359..2841
                     /locus_tag="IU325_15255"
     CDS             2359..2841
                     /locus_tag="IU325_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAP5810364.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(2918..3265)
                     /gene="rplS"
                     /locus_tag="IU325_15260"
     CDS             complement(2918..3265)
                     /gene="rplS"
                     /locus_tag="IU325_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP5810365.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(3307..4074)
                     /gene="trmD"
                     /locus_tag="IU325_15265"
     CDS             complement(3307..4074)
                     /gene="trmD"
                     /locus_tag="IU325_15265"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP5810366.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKKEHAQQQHKHDGMA"
     gene            complement(4105..4653)
                     /gene="rimM"
                     /locus_tag="IU325_15270"
     CDS             complement(4105..4653)
                     /gene="rimM"
                     /locus_tag="IU325_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP5810367.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4672..4920)
                     /gene="rpsP"
                     /locus_tag="IU325_15275"
     CDS             complement(4672..4920)
                     /gene="rpsP"
                     /locus_tag="IU325_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP5810368.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(5169..6530)
                     /locus_tag="IU325_15280"
     CDS             complement(5169..6530)
                     /locus_tag="IU325_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP5810369.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRYITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6622..7488
                     /locus_tag="IU325_15285"
     CDS             6622..7488
                     /locus_tag="IU325_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311501.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HAP5810370.1"
                     /translation="MQRLEQRSPDAILLLFLIAQTVDITMPVFALLALVAYSVSLALI
                     VPGLLQKNGGWRRMAIISAVIALVCHAIALEARILPDGDSGQNLSLLNVGSLVSLMIC
                     TVMTIVASRNRGWLLLPIVYAFALINLALATFMPNEYITHLEATPGMLVHIGLSLFSY
                     ATLIIAALYALQLAWIDYQLKNKKLAFNQEMPPLMSIERKMFHITQIGVVLLTLTLCT
                     GLFYMHNLFSMENIDKAVLSIVAWFVYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLA
                     YFGSRIVQQLIS"
     gene            7509..8795
                     /locus_tag="IU325_15290"
     CDS             7509..8795
                     /locus_tag="IU325_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF21 domain-containing protein"
                     /protein_id="HAP5810371.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8849..9442)
                     /gene="grpE"
                     /locus_tag="IU325_15295"
     CDS             complement(8849..9442)
                     /gene="grpE"
                     /locus_tag="IU325_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP5810372.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPDASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            9565..10443
                     /gene="nadK"
                     /locus_tag="IU325_15300"
     CDS             9565..10443
                     /gene="nadK"
                     /locus_tag="IU325_15300"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HAP5810373.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            10529..12190
                     /gene="recN"
                     /locus_tag="IU325_15305"
     CDS             10529..12190
                     /gene="recN"
                     /locus_tag="IU325_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP5810374.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALAVMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            12339..12680
                     /gene="bamE"
                     /locus_tag="IU325_15310"
     CDS             12339..12680
                     /gene="bamE"
                     /locus_tag="IU325_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HAP5810375.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12742..13032)
                     /locus_tag="IU325_15315"
     CDS             complement(12742..13032)
                     /locus_tag="IU325_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HAP5810376.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDTVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(13022..13498)
                     /gene="ratA"
                     /locus_tag="IU325_15320"
     CDS             complement(13022..13498)
                     /gene="ratA"
                     /locus_tag="IU325_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HAP5810377.1"
                     /translation="MILFVGFLLMEIVMPQISRTALVPYSAEQMYQLVNDVQSYPQFL
                     PGCTGSRILESTPGQMTAAVDVSKAGISKTFTTRNQLTNNQSILMNLVDGPFKKLIGG
                     WKFTPLSQEACRIEFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            13630..14112
                     /gene="smpB"
                     /locus_tag="IU325_15325"
     CDS             13630..14112
                     /gene="smpB"
                     /locus_tag="IU325_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP5810378.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            14327..14689
                     /gene="ssrA"
                     /locus_tag="IU325_15330"
     tmRNA           14327..14689
                     /gene="ssrA"
                     /locus_tag="IU325_15330"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            14856..16085
                     /locus_tag="IU325_15335"
     CDS             14856..16085
                     /locus_tag="IU325_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003845866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP5810379.1"
                     /translation="MARQTKKLTDTEIKNTKPADKEINLFDGDGLLLRIAPSSKGGKK
                     NWYLRYPVPVTKKRTKMSLGTYPHLSLAKARALRDEYLSLLANGIDPQAHITEKTNAL
                     KDATEHTLQAVARKWLDEKIKTAEITEDYAKDIWRSLERNIFPGLGDVPVKEIRPKML
                     KQHLDPIEQRGVLETLRRIISRLNEIFRYAATEELIEFNPADNLAQRFSKPKKQNMPA
                     LPPDELPRFMVALSNASIRLETRMLIEWQLLTWVRPGEAVRARWCDIDEESGFWNIPS
                     EFMKMKRPHKVPLSKEALRILENMRPISGHREWVFPSIKAPLSHMHEQTANAAIIRMG
                     FGGELVAHGMRSIARTAAEESGKFRTEVLEAALAHSKKDEIVAAYNRAEYLAERATLM
                     QWWGDYVQTQKFKALAA"
     gene            16658..>17397
                     /gene="dcm"
                     /locus_tag="IU325_15340"
     CDS             16658..>17397
                     /gene="dcm"
                     /locus_tag="IU325_15340"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP5810380.1"
                     /translation="MPYQTDIYRKVMSKAPVVIDLFCGCGGFGLGAKLAGFDVIAAVD
                     IDATLQSAYARNFPNTKIINGDISLLGESEWNSIILSKKIDGIIGGPPCQGYSRMGHS
                     DVNDPRRSLLRHFFRTVNIIKPKFFVMENVEGLMDEKNVYELKSALKILDADYQVLDP
                     MIIDASDFGAPTRRRRVVVIGFIPEEFECGLNSETLKAGNYERNTVRNAISDLPAPLM
                     KSKNNDITDFGWHKYRDVNVENLSKYAK"
BASE COUNT         4557 a         4426 c         4087 g         4327 t
ORIGIN      
        1 acaatttact cgcgcttttg atgcttattt cgtacaaata aatttacacc tcctttaaaa
       61 acaatccgct atgctttgaa aaaggagaaa gaaatgatga aaaagtttat cgcccccttg
      121 ttggctttac tggttagcgg atgtcagatt gatccttata ctcacgcgcc aaccttgacc
      181 agcaccgact ggtatgatgt cggtatggaa gatgcgatat cggccagcgc cataaaagat
      241 gacgatgcat ttagcgattc acaggcggat cgcggtctat accttaaagg atatgccgaa
      301 ggacaaaaga aaacttgcca gaccgatttt acttatgccc ggggcctttc cggtaaaagc
      361 tttcctgcca gctgcaacaa tgttgaaaat gccagtcaac tgcatgaagt atggcaaaaa
      421 ggggctgatg aaaatgccag cacaatacga ttaaattaat taacccccca caaataaatt
      481 aaacatgaga ttctactcat tgtgttttat ttctcacatt gatgacgacc gcatgaaaga
      541 atgatatttt aatgagcatt ctgaaaaaat atcgatgcat ttcgagcgaa gatggtgagg
      601 atccctgaat gcgtttttct caccgactgt tcttactgct gattctcctg ctgacgggag
      661 caccaatact cgctcaggaa ccttctgacg ttgcgaaaaa cgtgcgtatg atggtttccg
      721 gtattgtcag ttacacccgc tggcctgcgc tatcagggcc accaaagtta tgtatctttt
      781 catcttcaca ttttagtaac gccctacaag agaatggtac gacatctttg ccttatctgc
      841 ctgtcatcat tcacactcaa caagaggcga tgatttcagg ctgcaatggt ttttattttg
      901 gcaacgagtc accgacattc cagatggaat taaccgaaca atatccgtca aaggcgttat
      961 tattaattgc cgaacaaaat accgagtgca ttattggtag cgccttttgt ctgatcatcc
     1021 acaataatga cgtcagattt gccgtaaacc tggatgcctt atcgcgtagc ggtgtaaagg
     1081 tcaacccgga tgtcttgatg ctcgcacgga agaaaaatga tggataacga taattctctt
     1141 aataagcgcc ccacgtttaa aagagcatta cgcaacatca gtatgaccag catatttatc
     1201 actatgatgc tgatctggtt gctgctttcc gtgacctcgg tgctgaccct gaaacagtac
     1261 gcgcaaaaaa acctggcact gacagcagca acaatgactt acagtctgga agcagcggtc
     1321 gtttttgccg atggctctgc agcaactgaa acactggcag cgctggggca gcaaggccaa
     1381 ttttcaactg cagaagtgcg tgataagcag caaaatattc tggcatcctg gcattacacc
     1441 cgtaaggatc caggcgatac tttcagtaat ttcataagcc actggctctt ccctgccccc
     1501 atcgttcagc cgattcgtca caatggtgaa accattggcg aagttcgctt aaccgctcgc
     1561 gacagttcaa tcagccattt catctggttt tcgctcgctg tcctgaccgc ctgcattctg
     1621 ctggcatcag gcatcgcaat taccctcacc cgccatttgc acaatggtct ggttgaagcg
     1681 ttaaaaaaca tcaccgatgt cgtacatgat gtgcgttcca accgcaattt ttcccgacga
     1741 gtttcggaag aacgtatcgc agagtttcac cgcttcgctc tcgacttcaa cagtctgctg
     1801 gatgaaatgg aagagtggca gcttcgttta caggccaaaa atgcgcagct tctgcgtacc
     1861 gcgctacatg acccattaac cgggctggct aaccgcgcag cgtttcgtag cggtatcaat
     1921 acgttgatga acaattccga tgcccgaaaa acgtcggcgt tactatttct tgatggcgat
     1981 aatttcaaat atatcaatga tacctggggt catgcgacgg gcgatcgtgt cttgattgag
     2041 gtagcaaaac ggttagctga atttggcggg ctgcgacata aagcataccg cctgggcggc
     2101 gatgaattcg cgatggtgct ctatgatgta cagtcggaat ctgaagtgca gcagatatgc
     2161 tcagcactga cacaaatctt taatctcccg tttgatcttc ataatggcca tcagaccacc
     2221 atgacattaa gcattggtta cgcgatgacc attgagcacg cctctgcgga aaaattacaa
     2281 gagcttgccg atcacaatat gtatcaggcc aaacaccagc gtgccgaaaa gctggtgaga
     2341 taacaaggat atatcgatat gataaagcac ctggtagcac ccctgatttt cacctcacta
     2401 atactgactg gctgccagtc ccctcaggga aagtttactc ctgagcaagt cgccgctatg
     2461 caatcttatg gatttactga atccgccggc gactggtcgc tgggcttatc agatgccatt
     2521 ctgttcgcaa aaaatgacta caaattgctc ccggaaagcc agcaacagat ccaaaccatg
     2581 gcagccaaat tggcctcgac agggctaaca catgcccgta tggatggaca caccgataac
     2641 tatggtgaag atagttacaa cgaaggctta tcattgaaac gggcgaatgt cgtggccgat
     2701 gcatgggcta tgggtggaca aattccgcgc agcaatctca ccacacaggg tttaggaaaa
     2761 aaatatccca tagccagtaa caagaccgcc cagggccgcg ccgagaaccg ccgcgtcgca
     2821 gtggtgatta ctacccctta aaacacgagc aaacagataa aaaaagggcc agccaattgg
     2881 ccagcccttc ttaacaggat gtcgcttaag cgaaacctta gttaagacgc tctttgatac
     2941 gagcagcctt accagtacgc tcacgcaggt agtacagttt agctttacga acagcaccac
     3001 gacgtttgac agaaatgctg tcaactaccg gagagtgagt ctggaagaca cgctcaacgc
     3061 cttcgccgtt ggaaatttta cgaacagtga atgcagagtg cagaccgcgg ttacgaatag
     3121 cgataaccac gccctcgaat gcctgcagac gttttttgga accttcaaca acccatactt
     3181 tcacttccac ggtatcaccc ggacggaagg aaggtacgtc ctgcttcatc tgctcttgtt
     3241 caagttgctt aataatgttg ctcataattt aatctcttat cctgggtaaa ctgatatctc
     3301 gggggcttac gccatcccat catgtttatg ttgctgttgt gcgtgttcct ttttgaactc
     3361 cgccagcaac cttgcttgct cttcagtcag agccaggttt tccagaagtt caggtcttct
     3421 aagccaggta cggcccagcg actgtttcaa acgccagcga cgtatctcgg catggttgcc
     3481 cgacagtaac actggcggaa cttccatccc ttctaacacc tcaggccgcg tatagtgcgg
     3541 gcaatccagc aatccttcag caaaggaatc ttccgttgct gaggcttcat gtcccagtac
     3601 tcccggaata aaccgggaaa cggagtcaat cagcgtcatt gctggtaact caccaccact
     3661 gagaacgtaa tcgccgattg accattcttc gtcaatttcg gtttggatca cgcgctcatc
     3721 tataccttcg tagcgaccgc acaccagaat caatttttga ttcgttgcca gttcgctgac
     3781 gcccgcttga tcaagcttgc gcccctgtgg tgaaagataa atcacctttg cgccttcacc
     3841 cgccgcggct tttgctgcat gaatggcgtc ccgcaaaggt tgcaccatca ttaacatccc
     3901 cggtccgccg ccgtaaggac gatcgtccac ggtacggtgc cggtcatgcg tgaagtcgcg
     3961 aggactccag ctctggatgc tcagcaggcc atttttaact gcccggccag ttaccccgta
     4021 atcggtaatt gcgcggaaca tttcaggaaa caggctaatt atgccaatcc acatagcgcc
     4081 gtcttttacc gtttatccgg tggtttaaaa accaggatcc caatctactt cgattgaacg
     4141 agtagtgaga tcgactttct tgataacctg cccatcgagg aacggtacga gacgttcctt
     4201 gataccaaac gcatctttca ggtttgcctt aatgacgaga acgtcattag atccggtttc
     4261 catcatatcg acgactttac cgagatcgta gccttcagtg gttactacct ggcagcccat
     4321 caggtctttc cagtagtagt ccccctcttc aagctgaggc agctgcgatg aatccacgac
     4381 aatttcacaa ttcgtcagca ggttcgccgc atcacgatcg tcaacgcctt tcagcttgat
     4441 gatcatgtcc tgattgtggt gcttccagct ttccagctgg acttgctgcc actgacccgc
     4501 cttctggata aaccagggct gatagtcaaa aatgctttcg gcgtcttcgg tggaagaaaa
     4561 cactctgagc cacccacgaa taccgtaaga cgaacccatt tttcccaaaa cgatgggatc
     4621 aacaggtgct tgcgcggtga gttgtttgct catcatgacc accgtgacag attaagctgc
     4681 tttgtttact tctttgatca gcgcagcaac gcgatcagaa atagttgcgc cctggccaac
     4741 ccagtgagcg atgcgatcca gatccaggcg agtgccttct tctttttcgc tagcgattgg
     4801 gttgaagaaa ccaacgcgct cgatgaagcg accgttgcgt gcattacggc tgtcagcgac
     4861 aacaacctgg tagaacggac gctttttagc gccgtgacgt gctaaacgaa tagttaccat
     4921 aacatcctct tgtgtgaata aaacaaccgg accccatcga ggaacggagt ccggtgtcat
     4981 attaaaagcc cgaaaatttt actcattttt gcggaaattg caatcaacag ttgctaactc
     5041 tgctgtaaaa ggccgtcggc ggtgcagcca gtttggtgcc ggagtgcgcg cagtcaccgg
     5101 agcgtacacg cagtacgtga ggatgacgag cacaccccgg tgccaaaatg acaaacaagc
     5161 caggccgatt agcgaccagg aaatcccggc ggcatcatcc ccttcatgct tctcatcatc
     5221 ttcgccattc cgcccttctt cattttcttc atcatgcgct gcatgtcgtc gaactgcttc
     5281 agaaggcggt taacgtcctg tacctgcatc ccgcaaccgg cggcgatacg acgcttacgc
     5341 gaacctttga tgatttctgg cttagcgcgc tctttcatcg tcatcgagtt gatgatggct
     5401 tccatacgca ccagcacttt atcgtccatc tgcgacttga cgttatccgg gatctgcccc
     5461 atgcccggca gcttgcccat caggctagcc atgccgccca tatttttcat ctggcgcagc
     5521 tgctcaagaa agtcgttgag atcgaagccg tcgccttttt tcagcttgct ggctaatttc
     5581 tctgcctgcg cgcggtcaac tttgctttca atatcttcga tcagtgacag tacgtcgccc
     5641 atgccgagaa tacgcgacgc gatgcggtcc ggatggaacg gctccagcgc ctcagttttc
     5701 tcgccaacac cgaggaactt gatcggtttg ccagtgatgt aacgaataga gagcgccgca
     5761 ccaccgcggg catcgccgtc cactttggtc aacactacgc cggtaagcgg taacgcttca
     5821 ttaaatgctt ttgccgtatt ggccgcatcc tgaccggtca tggcgtcaac cacaaacagg
     5881 gtttcaaccg gattaatcga cgcatggact tgtttgatct cgtccatcat cgcttcgtca
     5941 acgtgcagac gaccagcggt atccaccagc agcacgtcgt agaatttcag tttggcttct
     6001 ttcagcgccg cgttaacaat atcaaccggc ttctgaccaa catcagaagg gaagaaatca
     6061 acgcccacct gctctgccag cgtctcaagc tgtttgattg ccgccgggcg ataaacgtcg
     6121 gcggaaacca ccagcacttt cttcttgtgc ttctcgcgca ggaacttacc gagtttacca
     6181 acgctggttg ttttaccggc accttgcagg cctgccatca gcacgaccgc aggcggttgc
     6241 gcagccaggt tcagggtctg gttctcttcg cccatcgccg caaccagttc gttacggacg
     6301 attttgacga actcctgccc cggcgtcagg ctcttattaa cttcatgacc aaccgctttc
     6361 tcttttacgc gattgataaa ctcacgcact accggcagag cgacgtccgc ctccagcagc
     6421 gccatgcgca cttcgcgcag cgtatctttt acgttgtctt cagtgaggcg tccacggcca
     6481 ctgatattgc gcagcgtgcg cgacaaacga tcggttaaat tatcaaacat tgtctctcgc
     6541 ctggggtgga aacggttggc cgcaatcgcg acacatcatc agtattttgc cgcagtataa
     6601 catgaaggcg tctttgttgt tatgcaacgg ttggagcagc gttcacctga cgctatactg
     6661 cttctctttc ttattgctca aactgtcgac attactatgc ccgtttttgc tctgctcgcg
     6721 cttgtcgcct actccgtcag tcttgcgctg attgttcccg gtctgctgca aaaaaacggc
     6781 ggctggcggc gcatggctat tatttctgcg gtcattgcgc tggtctgcca cgcaatcgct
     6841 ctggaagccc gcatcctgcc cgacggtgat agcggacaaa acctcagcct gctgaacgtt
     6901 ggttcattgg tcagtttgat gatctgtacg gtaatgacca ttgtggcttc tcgcaatcgt
     6961 ggctggttgc tgctacccat tgtctatgcc tttgcgctta tcaacctggc gctggcgacc
     7021 ttcatgccca atgaatacat cacccatctg gaagcaacgc ctgggatgct ggtgcacatt
     7081 ggcttatcgc tcttttccta tgccacgcta attatcgccg ccctgtacgc gctgcaactg
     7141 gcgtggattg attaccaact gaagaacaag aaactggcgt ttaaccagga aatgccgcca
     7201 ttgatgagta tcgagcgtaa aatgttccac atcacgcaga ttggcgtggt gctgctaacg
     7261 ctcacgcttt gcactggcct gttctacatg cacaacttat ttagcatgga aaatatcgac
     7321 aaggctgtgc tctctatcgt ggcgtggttt gtctatattg tgctgctgtg gggacattat
     7381 catgaaggat ggcgtggacg ccgcgtcgtc tggtttaacg ttgcgggtgc agtcattctg
     7441 acactggcct acttcggcag tcgaattgtc cagcagttaa tcagctaaac ccagaaaagg
     7501 agtttcccct ggaacacatt tctactacta cgttgatcat tattctgatc atcatggtgg
     7561 tcatttcagc ctatttttcc gggtccgaaa ccggaatgat gaccctcaac cgctatcgtc
     7621 tgcgacatat ggcgaaacag ggtaatcgct cggccaaacg cgtcgaaaaa ttgctgcgta
     7681 agccagaccg cctgattagc ctggtgttaa tcggcaataa cctggtcaat attcttgcct
     7741 ccgcgctcgg cactattgtt gggatgcgtt tgtacggcga tgcgggcgtg gcaattgcga
     7801 ctggtgtgct gacttttgtc gtactggttt ttgctgaggt attgccgaaa accattgccg
     7861 cgctgtaccc ggaaaaagtc gcttatccga gtagttttct gctggctccg ctgcaaattt
     7921 tgatgatgcc gctggtctgg ttgctgaatg ctatcacccg tatgctgatg cgcatgatgg
     7981 gtatcaaaac cgatatcgtg gttagcggct ctttgagcaa agaagagttg cgcactatcg
     8041 tgcacgaatc gcgctcacaa atttcccgtc gcaatcagga tatgctgctg tcggtgctcg
     8101 atctggaaaa gatgaccgtt gatgacatca tggtgccgcg cagtgaaatt atcggtattg
     8161 atatcaacga tgactggaaa tcgattctgc gccaactatc ccactcacct cacgggcgca
     8221 tcgtgctcta ccgtgattcg ctggacgacg ccatcagtat gctgcgagta cgtgaagcct
     8281 ggcggttgat gtcggagaaa aaagagttca ccaaagaaac catgctgcgc gccgcggacg
     8341 agatctattt tgtcccggaa ggtacgcccc tcagcacgca gttggtgaag tttcagcgca
     8401 acaaaaagaa agtcggcctg gtcgtcaacg agtatggaga cattcaaggg ctggtgacgg
     8461 ttgaagatat tctggaagag atagttggtg atttcaccac gtcgatgtcg ccaacacttg
     8521 ccgaagaagt cactccgcaa aacgacggtt cggtgattat cgatggcacc gccaacgtgc
     8581 gggaaatcaa caaagccttt aactggcatc taccggaaga tgatgcccgc acggttaatg
     8641 gcgtcattct tgaggcactg gaagagatcc ctgtcgcagg cacccgcgtg cgtattggcg
     8701 agtacgatat cgatattctc gacgtacagg acaatatgat taagcaggta aaagtttttc
     8761 ctgtaaaacc gctgcgcgag agtgtggcgg agtaaaaaaa cggcccggca ttcgcatgct
     8821 gggccgttat ttattacgaa aacagaaatt aagcttttgc cttcgctaca gtcaccatcg
     8881 ccgcacgaat tgtacgaccg ttcagcgtat aacccttctg cataatgccc agtacgttac
     8941 ctggcgcaac gtcatcagat tccaccattg cgatggcctg atgcacattc gggtccagcg
     9001 ggacgttagt ttcggcgatc acttcaacgc caaacttacg cacaacatcc agcatcgact
     9061 tcagcgtcag ctcaatacct tcaaccatcg cggacatatc cgggttagct ttatcagcca
     9121 cttccagcgc acgatccagg ctatcaatca ccggcagcaa ttcgttgatg aatttctcca
     9181 gcgcgaattt gtgggctttt tcaatatcca gttcagtacg acgacgcagg ttttccattt
     9241 cggcttttac gcgcaaaatg ccgtcacgtt cacgagtctg ggcttcagcc agctgagctt
     9301 cgagattcgc aattttttca tcgcgcggat ccacctgctc agcagaagcg tctggctcaa
     9361 ctgcctcaat ctcttcgtgc tgatccatga taatttcttc cggggcttgc ccctcaggcg
     9421 ttttctgttc tttactactc atgaatttct ccgcgttttt ttcgcattca tctcgctaac
     9481 ttcgcttatt atggggatca gtttcagggt ttcaagggaa gcactcacat tgtcatcaat
     9541 cttcgcaaca aggacctcgg aaaaatgaat aatcatttca agtgtattgg cattgtggga
     9601 cacccacggc accccactgc actgacaaca catgaaatgc tctaccgctg gctgtgcaca
     9661 aaaggttacg aggtcatcgt tgagcagcaa atcgctcacg aactgcaact gaagaatgtg
     9721 aaaactggca cgctcgcgga gattgggcaa ctggctgatc tcgcggtagt cgttggtggc
     9781 gacggtaata tgctgggcgc ggcgcgcaca ctcgcccgtt acgatattaa agttattgga
     9841 atcaaccgtg gcaacctggg tttcctgact gaccttgacc ccgataacgc ccagcaacaa
     9901 ttagccgatg tgctggaagg ccactacatc agcgagaaac gttttttgct ggaagcgcaa
     9961 gtttgtcagc aagattgcca gaaacgcatc agcacggcga ttaacgaagt ggtacttcat
    10021 cctggcaaag tggcgcatat gattgagttc gaagtgtata tcgacgagat ctttgcgttt
    10081 tctcagcgtt ctgatggcct gattatttcg acgccaacag gctccaccgc ctattccctc
    10141 tctgcgggcg gtcctattct gacaccctct ctggatgcaa ttaccctggt gcccatgttc
    10201 ccgcatacgt tgtcagcgcg accgctggtg ataaacagca gcagcacgat ccgtctgcgt
    10261 ttttcgcatc gccgtaacga cctggaaatc agttgcgaca gccagatagc actgccgatt
    10321 caggaaggtg aagatgtcct gattcgtcgc tgtgattacc atctgaatct gattcatccg
    10381 aaagattaca gttatttcaa tacattaagc accaagctcg gctggtcaaa aaaattattc
    10441 taattttacg ccagcctctt tactgtatat aaaaccagtt tatactgtac acaataacag
    10501 taatggtttt tcatacagga aaacaactat gttggcacaa ctgaccatca gcaactttgc
    10561 tatcgttcgt gagcttgaga ttgattttca tagcggcatg accgtaataa ctggcgagac
    10621 cggcgcgggt aaatctattg caatagatgc cctcggcctt tgtctcggtg gtcgcgctga
    10681 agccgacatg gtgcgtaccg gcgctgctcg tgccgacctg tgcgcccgtt tttctctgaa
    10741 agatacgcca gcggccctgc gctggctgga agaaaaccag cttgaagacg ggcatgaatg
    10801 tttgcttcgt cgcgtaatca gcagcgatgg tcgctcccgt ggttttatca acggtacagc
    10861 ggttcctctt tcacaactgc gcgaactggg tcagttgctg attcagatcc atggtcagca
    10921 cgctcatcag ttactcacca aacccgagca ccaaaaattc ctgcttgatg gctatgccaa
    10981 tgaaacctct ctactccagg aaatgaccgc acgttatcag ttgtggcatc aaagctgccg
    11041 tgacctcgcg catcatcaac agttaagtca ggaacgcgcc gcccgtgcag aactgctgca
    11101 ataccaatta aaagaactta acgaatttaa tccgcagccc ggagagtttg agcaaatcga
    11161 cgaagagtac aaacgtctgg cgaacagcgg tcaattgctg actaccagcc agaatgcatt
    11221 ggcagtaatg gccgacggtg aagacgcaaa cctgcaaagt cagctttaca cggctaaaca
    11281 actggtgagc gaattgattg gcatggacag caaactgtcc ggcgtacttg atatgctgga
    11341 agaagctacc atccagattg ctgaagccag cgatgaactg cgccactact gcgatcgtct
    11401 ggacctcgat cctaaccgac tgtttgaact tgaacagcgc atctcaaaac agatttcgct
    11461 ggcacgtaaa catcacgtca gccctgaggc attgccacag tattaccagt cgctactgga
    11521 agaacagcag caactggacg atcaggccga ctcacaagaa acgctcgcgc tggcggtaac
    11581 gaaacatcat cagcaggcac tggaaacggc gcgcgcgtta caccagcaac gccagcacta
    11641 tgcagaagaa cttgcacagc tgatcaccga cagtatgcat gcactctcta tgccacatgg
    11701 gcagtttacg attgatgtta aatttgacga gcatcacctg ggcgctgacg gtgccgatcg
    11761 tattgagttt cgggtaacca ccaacccagg tcagccaatg cagcctattg ccaaagttgc
    11821 atccggtggt gaattgtccc gcatcgcatt ggcaattcag gtcatcacgg cgcgtaaaat
    11881 ggaaaccccg gcactgattt ttgatgaagt ggatgtaggg attagcggcc caacagcggc
    11941 agttgttggc aaactgctgc gtcaactcgg cgaatcaact caggtgatgt gtgttaccca
    12001 cctgccacaa gtcgcgggat gtggtcatca acactatttt gtcagcaaag aaaccgatgg
    12061 tgcgatgaca gaaacgcata tgcaatccct ggataaaaaa gcgcggttac aagagctggc
    12121 gcgcctgctt ggtggcagtg aagtcacacg taacacactg gcgaatgcga aagaactgct
    12181 tgcggcgtaa acttttttcc tgcttcacgg tcagagtaaa cagcaaaacg ccgtaagacc
    12241 ggaaagcaaa aggttttaaa gtgatgaaag gtctattatc atcggcatat tacagatgag
    12301 ccacgtactg ctcgggcccg aaaaggaatc aaatcactat gcgctgtaaa acgctgactg
    12361 ctgcagcagc agtactattg atgttgaccg caggctgttc cactctggag cgagtggttt
    12421 accgtcctga catcaaccag gggaactatc tgaccgctaa cgacgtatcc aaaatacgtg
    12481 ttggcatgac gcaacaacaa gttgcgtacg cattgggtac accgctgatg tccgatccat
    12541 ttggtacgaa tacctggttc tatgtcttcc gccagcaacc aggtcatgaa ggtgtaacgc
    12601 agcaaacgct gacgctgacc tttaacagta gcggagtgtt gaccaacatt gataacaaac
    12661 ccgcgctgag tggtaactaa tcataaagtt gccctcaaag acgttaaaaa aggtgctcaa
    12721 tgagcacctt ttttctgtct gttatttatt cgctgatttt tctgctcgtt gcctgcgcag
    12781 ctctttcgga tcggcaatga gaggacgata aatctccacc cgatcgccat catgcacggt
    12841 atcgctgagt tttgccggac ggctgtaaat gccgactttg tttttagtta aatcgatatc
    12901 ggtacgcaat tccagcaagc cactggcgcg aatagcttct tcaaccgtcg cgccctcctg
    12961 cagcgtcacg cgctgcaggt actgcttctc aggtagcgca taagccacct caacggcaat
    13021 tttacctggc actgtaaacc tctttcgccc gaaccgtaaa agcctggacc atattagccg
    13081 ccagctcttt aaacacgcga ccaaaggcga gttcaatcaa cttattggta aactcaaagt
    13141 cgagatgaaa ctcgatacga cacgcctcct ggctcagcgg cgtaaacttc cagccaccga
    13201 tcaatttctt gaacggccca tccaccagat tcataagaat actttggtta ttggtcaact
    13261 ggttgcgggt agtaaacgtt ttgctgatcc cggccttaga gacatctacc gccgcagtca
    13321 tctgcccagg agtggactcc agaatccgac ttccggtaca acccggcaaa aactgaggat
    13381 aagactgaac gtcattcact aactgataca tttgctccgc gctgtagggt accagtgcgg
    13441 tccggctaat ctgaggcata acaatttcca tcaacaaaaa tccaacaaat aatatcattt
    13501 atcctgctgt aaaaaaaacg ctatcccggc gctgggtaac atcgggttca tgctaagata
    13561 gagccttgtc ccccgcagga ttgatatggg gtgttttcga tttcagatta ccgatgattc
    13621 acgacgctta tgacgaagaa aaaagcacat aaacctggtt cagcgaccat cgcgcttaac
    13681 aagcgcgccc ggcacgaata ctttatcgaa gaagagttcg aagcgggact tgccctgcaa
    13741 ggctgggagg ttaaatccct gcgcgcagga aaagccaata tcagcgacag ctacgtcctt
    13801 ctacgtgatg gagaagcatt tctgtttggc gctaacatca cgccaatggc tgtggcctcc
    13861 acgcatgtgg tgtgcgatcc tacccgtacc cgcaagttac ttctcaacca gcgcgaactg
    13921 gactcattgt acggtcgcgt caatcgagaa ggctataccg tagtggcgct ctccctgtac
    13981 tggaaaaatg cctggtgcaa agtgaaaatc ggcgtcgcca aaggtaagaa acagcacgat
    14041 aaacgttcag atatcaaaga gcgcgaatgg caggtggata aagcgcgtat catgaaaaac
    14101 gcccaccgtt aaacctgcac tccaattatt gaccagttcc tcaccgcgcc tccctctccg
    14161 gcggcgcgaa tgaacatctt attggctatc acatccgaca caaatgttgc catcccattg
    14221 cttaatcgaa taaaaatcag gctacatggg tgctaaatct ttaacgataa cgccattgag
    14281 gctggtcatg gcgctcataa atctggtata cttgccgtta cacattgggg ctgattctgg
    14341 attcgacggg atttgcgaaa cccaaggtgc atgccgaggg gcggttggcc tcgtaaaaag
    14401 ccgcaaaaaa tagtcgcaaa cgacgaaaac tacgctttag cagcttaata acctgcttag
    14461 agccctctct ccctagcctc cgctcttagg acggggatca agagaggtca aacccaaaag
    14521 agatcgcgtg gaagccctgc ctggggttga agcgttaaaa cttaatcagg ctagtttgtt
    14581 agtggcgtgt ccgtccgcag ctggcaagcg aatgtaaaga ctgactaagc atgtagtacc
    14641 gaggacgtag gaatttcgga cgcgggttca actcccgcca gctccaccac tttttagtaa
    14701 aacaaagtct actaaagact atcaaggccg cacctcgcaa gggttgcggc ctttttcatg
    14761 tctagcgaag tctactgaga attgctagaa gctacgcatt atgggaccct tatatggacc
    14821 caccggttgg gtccaataat ttgagggtcc aatacatggc aaggcagact aaaaaactca
    14881 cagacactga aatcaaaaac accaagccag cagacaaaga gatcaatctc ttcgatggtg
    14941 atgggctact gctacgaatc gctccttcat caaaaggagg gaagaagaac tggtatctca
    15001 gatatccagt accagtgacc aagaagcgca ctaagatgag cttgggaacc taccctcacc
    15061 tctcattagc aaaggcaaga gctttacgtg atgaatatct gtctttgctt gctaatggta
    15121 ttgaccctca agcccacatc actgaaaaaa ctaacgctct gaaagatgcc acggagcata
    15181 cgctacaagc tgttgcccga aagtggttag atgagaagat aaaaaccgct gaaataacgg
    15241 aagactatgc aaaagatata tggcgtagtc ttgaacggaa catttttccc ggcttgggcg
    15301 acgttcctgt caaagagatc cgccccaaaa tgctcaagca gcatctagac ccaattgagc
    15361 aacgaggtgt acttgaaact ctacgccgca tcatttctcg cctgaatgaa atttttcgct
    15421 atgctgccac ggaagagctt atagagttca atcctgccga caatctcgct cagcgtttca
    15481 gtaagcctaa gaagcaaaac atgcctgccc ttccacccga tgagctacca cgcttcatgg
    15541 ttgctttatc taacgcatct atacggttag aaacccgtat gctgatcgag tggcaattat
    15601 tgacgtgggt aaggcccggt gaagccgttc gggcaagatg gtgtgatatt gatgaagaga
    15661 gcggattttg gaatatccct tcagaattca tgaaaatgaa acgcccacat aaggttccat
    15721 tgagtaaaga agctcttcgc atacttgaaa acatgcgccc tatcagtggt catagagaat
    15781 gggttttccc cagcatcaaa gctcccctca gtcatatgca tgagcaaaca gcaaacgccg
    15841 ccataatcag aatgggattc ggtggtgaat tggtagctca tggtatgcgg tctatcgcca
    15901 gaacagcagc agaggagtct ggtaagttca gaacagaagt tctcgaggcg gctcttgctc
    15961 actcgaagaa agatgagatc gtagccgctt ataaccgtgc cgagtatcta gcagaacgag
    16021 caacactaat gcaatggtgg ggtgattatg ttcagacgca gaaatttaag gctctagcgg
    16081 catgataatg aaaatactgc atggattcag atcaacatgc cgagattgat ttgacgaaac
    16141 tactaactaa ccgtgttaga gtcattgaag tcaccagttg gcagacaacg tcaaagtagc
    16201 ttatcaacat aggactttat ggcctaaact ggtggcacta atggataact ggaccacttg
    16261 ccttgcaaat gcctagctgt cgatttaaca ccatcaataa tacacatgat caaaggtgct
    16321 atgacaacga tgcagacccc gtggtcattg cttctgcata cccgattgag tctggattac
    16381 cgtcagtgga cgctgctaag tgccagatga gcaatcagaa gcgtaacagt gtcgtgctgc
    16441 ttacatcaat cttgctgaac atctcgatga gcgcaggttg atgctacaaa agcaggcata
    16501 aaattcgaat atgataacgc aacaaaaata cattactcat tgattttaaa tgattttccc
    16561 actccacaag gaaatgtaaa tatcacatct atattcccat aagtttagaa agttgctaaa
    16621 atatctctta atattaaggg catagtaaag aataacagtg ccctaccaaa cggatattta
    16681 taggaaagtt atgagcaaag cacccgtagt aatagatctt ttttgcggat gtggtggttt
    16741 tggacttgga gccaagttag caggctttga tgtcatagcc gctgttgata tagatgctac
    16801 tttgcagtca gcttatgccc gcaattttcc aaatactaaa attataaatg gcgatatttc
    16861 attattggga gaatctgaat ggaactcaat aatattgtca aagaaaattg atggaataat
    16921 aggtggcccc ccttgtcaag gctacagccg aatggggcat tctgacgtta atgatccacg
    16981 ccgttcactt ctgagacatt tttttagaac agttaatata ataaaaccta aattttttgt
    17041 tatggaaaat gttgaaggct taatggatga gaaaaacgta tatgaattaa aatcagcact
    17101 aaagatattg gatgctgatt atcaagtcct agaccctatg atcattgacg cttctgactt
    17161 tggtgcacca acgagaagac gcagggttgt ggttataggt tttattcctg aagagtttga
    17221 atgtggatta aatagtgaga cgctcaaagc tggtaattat gagagaaata ctgtcaggaa
    17281 tgcgataagt gatcttcccg caccactaat gaaatcaaaa aacaatgata taactgattt
    17341 cggttggcac aagtatagag atgtaaatgt agaaaattta tcaaaatatg caaaaga
//
LOCUS       DACBVI010000096        16720 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.096, whole genome shotgun sequence.
ACCESSION   DACBVI010000096 DACBVI010000000
VERSION     DACBVI010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.096"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(253..789)
                     /locus_tag="IU325_15345"
     CDS             complement(253..789)
                     /locus_tag="IU325_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP5810381.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSAGYYVLIWQPLSERIEQQETILQQLAAMNTRLKSAAPDIIAARKSATTTPAQV
                     SRVISDSASVHSVVIKRIAERGENIQVWIEPVVFNDLLNWLKALDEKYALRVTQIDVS
                     AAEKPGMVNVQRLEFGRG"
     gene            complement(791..1969)
                     /gene="gspL"
                     /locus_tag="IU325_15350"
     CDS             complement(791..1969)
                     /gene="gspL"
                     /locus_tag="IU325_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HAP5810382.1"
                     /translation="MSSMLEIFIPLCASDPIRWQHRTTDVEHGIWSDVDNEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQANGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGRTLAPVMFRAQLNEQASPISLTVVGIAPEELSAWAGPDAERLTVTALPAITTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRAQA
                     EKQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALAADYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(1966..2943)
                     /gene="gspK"
                     /locus_tag="IU325_15355"
     CDS             complement(1966..2943)
                     /gene="gspK"
                     /locus_tag="IU325_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HAP5810383.1"
                     /translation="MITLPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPIAVQQLIALISRLNVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(2940..3545)
                     /gene="gspJ"
                     /locus_tag="IU325_15360"
     CDS             complement(2940..3545)
                     /gene="gspJ"
                     /locus_tag="IU325_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HAP5810384.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSVVADH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESGGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTTQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRITLHSPQWGEIERIWLLRGPQLS"
     gene            complement(3542..3913)
                     /gene="gspI"
                     /locus_tag="IU325_15365"
                     /pseudo
     CDS             complement(3542..3913)
                     /gene="gspI"
                     /locus_tag="IU325_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
     gene            complement(3910..4473)
                     /gene="gspH"
                     /locus_tag="IU325_15370"
     CDS             complement(3910..4473)
                     /gene="gspH"
                     /locus_tag="IU325_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HAP5810385.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSESPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRHGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLSQCDERMP"
     gene            complement(4477..4932)
                     /gene="gspG"
                     /locus_tag="IU325_15375"
     CDS             complement(4477..4932)
                     /gene="gspG"
                     /locus_tag="IU325_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HAP5810386.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADARNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(4949..6172)
                     /gene="gspF"
                     /locus_tag="IU325_15380"
     CDS             complement(4949..6172)
                     /gene="gspF"
                     /locus_tag="IU325_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HAP5810387.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RLNASTGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRALIAM
                     SDALQASGVYWLAGLLGLLVLGQRLLKNPAMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRATLAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(6172..7665)
                     /gene="gspE"
                     /locus_tag="IU325_15385"
     CDS             complement(6172..7665)
                     /gene="gspE"
                     /locus_tag="IU325_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HAP5810388.1"
                     /translation="MVPVAQETSANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNASFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSHELTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(7665..9725)
                     /gene="gspD"
                     /locus_tag="IU325_15390"
     CDS             complement(7665..9725)
                     /gene="gspD"
                     /locus_tag="IU325_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HAP5810389.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGTTTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPNTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(9755..10714)
                     /gene="gspC"
                     /locus_tag="IU325_15395"
     CDS             complement(9755..10714)
                     /gene="gspC"
                     /locus_tag="IU325_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HAP5810390.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSLNTDKEHLRKIVRAM
                     FWLMLLIISAKVAHSLWRYFSFSAEYTAVSPSANKPPRADAKTFDKNDVQLISQQNWF
                     GKYQPVAVPVKQPEPAPVAETRLNVVLRGIAFGTRPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLAEEERSTVAVTNKKAISDEAKQAVAEPAASAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            complement(10732..11142)
                     /gene="gspS2"
                     /locus_tag="IU325_15400"
     CDS             complement(10732..11142)
                     /gene="gspS2"
                     /locus_tag="IU325_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HAP5810391.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGAQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(11208..12017)
                     /locus_tag="IU325_15405"
     CDS             complement(11208..12017)
                     /locus_tag="IU325_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP5810392.1"
                     /translation="MFFDVFQQYPAAMPVLATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSTQSKISLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASVIDLDNQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGSW
                     VGALSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATLYLQALF"
     gene            complement(12157..>16720)
                     /gene="sslE"
                     /locus_tag="IU325_15410"
     CDS             complement(12157..>16720)
                     /gene="sslE"
                     /locus_tag="IU325_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HAP5810393.1"
                     /translation="KKFKYKKSLLAAILSATLLAGCDGGGSGSSSDTPPVDSGTGSLP
                     EVKPDPTPNPEPTPEPTPDPEPTPEPTPDPEPTPEPEPEPVPTKTGYLTLGGSQRITG
                     ATCNGESSDGFTFTPGDKVTCVAGNNTTIATFDTQSEAARSLRAVEKVSFSLEDAQEL
                     AASDDKKSNAVSLVTSSNSCPANTEQVCLTFSSVIESKRFDSLYKQIDLAPEEFKKLV
                     NEEVENNAATDKAPSTHTSPVVPVTTPGTKPDLNASFVSANAEQFYQYQPSEIILSEG
                     RLVDSQGYGVAGVNYYTNSGRGVTGENGEFSFSWGETISFGIDTFELGSVRGNKSTIA
                     LTELGDEVRGANIDQLIHRYSTTGQNNTRVVPDDVRKVFAEYPNVINEIINLSLSNGA
                     TLDEGEQVVNLPNEFIEQFKTGQAKEIDTAICAKTGGCNQARWFSLTTRNVNDGQIQG
                     VINKLWGVDTSYKSVSKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARNDKNY
                     WLAFGEKRAWDKNELAYITEAPSLVEPENVTRDTATFNLPFISLGQVGEGKLMVIGNP
                     HYNSILRCPNGYSWNGGVNKDGQCTLSGDSDDMKHFMQNVLRYLSDDKWTPDAKASMT
                     VGTNLDTVYFKRHGQVTGNSAEFGFHPDFAGISVEHLSSYGDLDPQEMPLLILNGFEY
                     VTQVGNDPYAIPLRADTSKPKLTQQDVTDLIAYLNKGGSVLIMENVMSNLKEESASGF
                     VRLLDAAGLSMALNKSVVNNDPQGYPNRVRQQRATGIWVYERYPAVDGALPYTIDSKT
                     GEVKWKYQVENKPDDKPKLEVASWLEDVDGKQETRYAFIDEADHKTEDSLKAAKAKIF
                     EKFPGLKECKDPTYHYEVNCLEYRPGTGVPVTGGMYVPQYTQLSLNADTAKAMVQAAD
                     LGTNIQRLYQHELYFRTNGRKGERLSSVDLERLYQNMSVWLWNKIEYRYENDKDDELG
                     FKTFTEFLNCYANNAYSEGTQCSADLKKSLVDNNMIYGDGSSKAGMMNPSYPLNYMEK
                     PLTRLMLGRSWWDLNIKVDVEKYPGAVSAEGEKVTETISLYSNPTKWFAGNMQSTGLW
                     APAQKEVTIESTASVPVTVTVALADDLTGREKHEVALNRPPKVTKTYDLKANDKVTFK
                     VPYGGLIYIKGNSPKNESAEFTFTGVVKAPFYKDGEWKNALNSPAPLGELESDSFVYT
                     APKNNLNASNYKDKGGLKQFAKDLDTFASSMNDFYGRNDEDGKHRMFTYKNLTGHKHR
                     FTNDVQISIGDAHSGYPVMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPGA
                     TEVANNVLALYMQDRYLGKMNRVADDITVAPEYLEESNGQAWARGGAGDRLLMYAQLK
                     EWAEKNFDIKTWYPDGNLPEFYSEREGMKGWNLFQLMHRKARGDEVGNNKFGSKNYCA
                     ESNGNAADTLMLCASWVAQTDLSEFFKKWNPGANAYQLPGATEMSFEGGVSQSAYNTL
                     ASLDLPKPKQGPETINKVTEYSMPAE"
BASE COUNT         3684 a         4753 c         4237 g         4046 t
ORIGIN      
        1 aatgatttat tgcatctatt aataatcata atgtgtcaaa acacttaagc aactttgcag
       61 tctgagaaag gtgtcgatcc acaaggacta catagtgaaa aagaattcct acagtattgt
      121 cgaacattcc gtggtggtct ggagggatat aattcttgct acatcggaac tcagcccggt
      181 gagcgggttt gacagttccg cactcaccat tgggctaagg gttatcaggt ggggttaagg
      241 aaatggcaat acctaccccc gtccaaactc cagtcgctgc acattcacca tcccaggctt
      301 ctcagcagca ctgacatcaa tttgtgtcac ccgcagcgca tatttttcat ccagtgcttt
      361 taaccagttc agcaggtcat taaacaccac aggttctatc cagacctgaa tattctcccc
      421 gcgctcggct attcgcttga tgaccacaga gtgcacggaa gcactgtcac tgatgacccg
      481 tgatacctgc gcaggcgttg ttgtggcaga ttttcgcgct gcgataatat ccggtgcggc
      541 gctcttcagt cgggtgttca tcgccgccag ctgctgcaat attgtctcct gttgctcaat
      601 ccgttcgctc aaaggctgcc agatgaggac gtaatatccg gcgctaaaca ggaacaccac
      661 cgctgccagt aacatgccct tttcacgcgg cgaacgcccc gccaggtgtt gtgccagcca
      721 gtgttcgcca cggcttaact ggcgttcacg ccattgctga aaatagtgaa taaatttatc
      781 gcgtaacatg ttatttcctc cgcaacgtta cgccgccgga aaccgcatca ccctctttct
      841 gtaacgcgtc ctgttgcaca acataatctg ccgccagtgc gctacgcagt ttgtcgaagc
      901 tggcaaagtt cgcagcccgt agctggaggt gaagcgtctg gcgtttttga tcaaaggtga
      961 aaccacgcat ctcgatgtcg gaaagtgacg ctgatttcag ggtactggcg atcgctgaca
     1021 attctgcgag cagccgggta tcgtcggcct gtgggcgata ttttttcagc gccatcgtca
     1081 cctgagagcg taaattcaca atcctttttt gttccgggaa cagcgttaag aactgtttct
     1141 ccgcctgggc gcggctttgc gccacctgtt cgctgacact ccataacgtc acgccccgtt
     1201 ccactgccag cgcaaccaga atcagcaata tcggcagaat catcacccgc cagcgcgccc
     1261 actgttttcg gtagctgacg cggggctgcc acggccctgt tagcaggttc ccttccggtt
     1321 cgccataagt ggtaatggcg ggcagagccg taacggtcag acgttcagcg tctgggccag
     1381 cccatgcaga tagctcttcc ggcgcgattc cgactacggt cagcgaaatc ggtgacgcct
     1441 gctcattgag ctgggcgcgg aacatgaccg gggccagcgt ccgcccggcg ctccatcccc
     1501 ggcattcatc gatgcggcag ataacccgtt gcgcatcgcc agccataaac ccacaaggaa
     1561 tggacatcca gtccggcgcg acggtagcgc gggtgatgcc gtttgcctgc aaccactgcg
     1621 caatgttgcg catatgctgt tggtgaatca ctgctacggt tgccagttgc tggtcgattt
     1681 tcaacggggc gaaatgcagt tcatcgatat cctggttcag ctcttcttcc agtaaggcgg
     1741 gcagaatagt cggtatctgc ttgcgggcga catcaggcag ttcaacctgc cagacgctga
     1801 tccattcgcc gggaatgtag agtcgaatcg catcagtttg cagccattgc tggagctgtt
     1861 cgttgtcaac gtcagaccag atgccgtgct ccacgtcggt ggtacggtgc tgccaacgga
     1921 tgggatcgga ggcgcaaagc gggataaaaa tctcaagcat ggaactcact cactttctcc
     1981 tgtctgatgc cagagaacag aaaagtgttg tgggcccatg cggacaatta acgaattcat
     2041 cgtcagttca atctcattca cggtgatatc tgaacgcagc cagaagtaat tgctgtccac
     2101 gctcaggacg gtttttagct gttttttagt acgctcatcg acgtcagcaa gtagcggctg
     2161 cgcaagaaac tgatcgacat cttcccagcc tttcgccgga cgttgttgta ataacgcccg
     2221 cgcctgaaca gggcttaacc acgggtcaaa cagcgcctca agaatcacac tttgcgtgac
     2281 gtctaaggta ttgatgttga tttgctggcg ggtcatcggc agcgcacaga ccagcggttt
     2341 cagtttttga taaagtccgg cgtccattcc ctgcaccacg cgcatctcgc tgatatcagc
     2401 cagcggttga ttggcagcgt aaaacggcac cgaacgggcg agatactcgc tgtcttcacg
     2461 gcccagacgc gtctgcacgc tgcggtcttc gtcaataaac tcccacaggc tttcggctat
     2521 cagttcggcc cgataagcag gcacattgag gcgtgatatc agggcaatca gttgttgtac
     2581 cgcaatagga cgcgacgccg tcgtcggctg agcgagggca ttcaggttaa agcaggcctg
     2641 ggcgtcacgc agagtgacgg cgatttgccc ctgcggcagt gggaaaaaac gagggccgga
     2701 agcccacggc tgcgccagat gcacgcgctt ttcatttttc aggctcagac tgagcgcgct
     2761 cagcgccagg ctttccgcac tggcgctgta ccacagcgcc tgctggtact cctgctggct
     2821 gcgcgttcgc ccaagttgtt gctgcattcg cccggaaagc gtgatggtca ccagcatcat
     2881 aaccgccagc aataccagca ccacgaccag tgccattccg cgttttggtg gtaaggtgat
     2941 catgataatt gcggcccgcg taacaaccag atgcgttcaa tttcgcccca ttgcggcgaa
     3001 tgcagggtta tgcgtactgc cacaggaatc gcctgcactg atgaccagct ctcctgccag
     3061 cgcgtgccgt cgtagaactg caaacggagc gaatccgccg ggattaattt ttgcgttgtt
     3121 ggcttcacgc tgcctgccgc atcggtcagt ggccaggcta accgttcgag atagccgtca
     3181 tgaatgcggt aaccgacggt gagcagatta ctgcgcggca gacgcatcaa cggattaacc
     3241 acgccgccgc gcacaaaacg cattcctcca ctctcagacg ccagaacgcc agcgcccgcc
     3301 agtaacgctg gttcacgctg gccctgatcg cctcttaccg gacgcggcat catttgtgtc
     3361 agatcgtggg tcagaaaact catcgtttgc tgcatgaggt ttagtttttg atcgtgatcg
     3421 gcgacgacac tattcacgcg tgtgacgccg ttagtcacct gctgcgccat cagtgccagc
     3481 gaggcaaaaa tggctatcgc caccagcatt tccagtaacg tgaaaccagc gcgagtcctt
     3541 ctcactgttg gcctcccacg gcgctaaacc aggcgcgtcg taactgaatc accgacgaaa
     3601 agtcttcgtg acggctgact tcaatatcca ctgcctggag cagcgcgtta cctgtattca
     3661 gcggtgtggt tcaccagtac cagcggcttc ctgccatatc actctcgccc tggtgcctga
     3721 ccgcccgctg ctcgcgggtc atcaggtaaa gcagcgcggt ctggttttca gctacccagc
     3781 ccgctaccgt tttttcctca agaacgtgct gattactcag cgcgctgctg gcaatctgta
     3841 acaccgccgt ggcagccagc gcaaaaatcg ccagcgcgag catcacttcc agcaaggtaa
     3901 atccacgctt catggcatcc tctcatcaca ttgactgagg gataacgcgc catcgtgtgc
     3961 cagttttacc gcccaacatg cgttttgcgc cgccgagtag aagcgcagcg taaacggcgt
     4021 ggcgggttca aaaggcgaaa aacggatctg cggcgtcttc tttttcgcct ctttttgcag
     4081 ctccagttca atatcgtgca gcgtcaggcg acgacgctgc agctccagcg catactcctt
     4141 ctgccagata tcactgccgg gttgtaacag catctgcacc tgttttggca ccgtaacctg
     4201 tgccgataac cgggtcgcag aaacgggcag ccactgcccg tgacgacgct gcataaactg
     4261 atagccaggc gggtcgatta gtacaccgag tgtttgccct tcgatcactg ccctgtcctt
     4321 aaactgcgca aagcgagtca gaaaatcctg cgccgctttt ttcgcgggcg attcagaatc
     4381 ggtcgcaaac gtctgtacca cgcccgcact ggcaaggccg ataaggaaaa tcaccagcat
     4441 gatttccaga agtgtgaatc cgcgttcagg cactgcttac tgaaactcct gcaaattcca
     4501 gttaccgata tctgcgccag cgccttcccc gttttcctga ccatctgccc ccagggtata
     4561 aacatcaaac agcccttttt cacccgggct gagatactga taatcattgc cccacggatc
     4621 ttttggcagt cgtttaatgt atccaccggt acggtagttg cgggcgtccg ccatattggc
     4681 cggttgctgg atcaacgcct ccagtccctg ttcggtggtc ggataacgcc cgttatccag
     4741 tcggtacata tccagcgcat tctccagcgc cacgatatcg ctgatggctt tttgccgatc
     4801 tgctttctct ttgttgccca acaggttagg caccaccaga cttgccagga cgccaagaat
     4861 aacaatcacc accatcactt ccagcagggt aaaacctgcc cgtggttttt gtgtgcggga
     4921 taacgaattc atgtgataac tccgtaaatt acattccaac catattgttc agttgcagca
     4981 tcggctcgag gatggcgatg acgataaaca gcaccacgcc cgccatcatc accaccagag
     5041 caggctcaaa cagccccaac gccagcccca cctgggtatc aaattctcgt tcctggttaa
     5101 ctgcggcctg ttcaagcatg gtttccagtt caccgctctg ttcgccggag gcgatcatgt
     5161 acagcatcat cggtgggaac aggcgcaact ccgccagcgt agcgcgcaga ctgcttcctt
     5221 cgcggacgcg atctgccgcc agcagcagtt gttgctcgac ataacgattt gccgacacgg
     5281 cggcagcggt ctgaatgcct tccagtagcg gaacactgct ggcggtgagg atgcttaatg
     5341 tgcgggaaaa ccgcgccgta ttcagcccgc gcgcaacacg ccccgtcaca ggcaggcgca
     5401 gcaaggtttt atcccagcgc aggcgcatcg caggattttt gagtagccgt tgccccaaca
     5461 ccagaagccc cagcaatccc gccagccagt aaacgccgct ggcctgtaac gcgtcactca
     5521 tggcgataag cgcgcgggtg gaggcgggta gcgcgtgtcc gaggtgatcg aactgttcga
     5581 tgattttcgg caccactgcc gtcagcaaaa tagtgaccac gcccgttgcc accaccagca
     5641 ataccagcgg gtagagcatg gcctgcaata gacgtgattt caggcgctgt cgctgttcgg
     5701 tgtaatccgc caggcgatta agcaccacgt cgagatgtcc ggatttttct ccggcagcga
     5761 ccatcgaaca aaacagagaa tcaaagacgc ggggatgttc gcgcaggctg tccgacaggg
     5821 tataaccttc ctgaatccgg ctgcgcagcg ccattccgag gctttttaca tgcagttttt
     5881 cactttgctc actgaccgcc tgtaagcagg tttccagcgg cattgctgcc tgtactaacg
     5941 ttgccagttg gcgtgtgaac agcgccagat ctgccgccgc cacgcgacga tgtgcgtgcc
     6001 gccgacgctg caacattccc cctgtcgaag cattcagcct ggcttcaatg tgcacgggga
     6061 taagctcttt accgcgcaac aactggcggg catgacgcgc tgaatccgcc tcaatcatac
     6121 ctttggtttt gcgaccatta cgctccagcg cctgatagta aaacagtgcc attacgcctc
     6181 catggttacc cgcagaactt catcgagaga ggtttctccg gcgagcactt tctcaatgcc
     6241 gttactgcgg atacccgcag agtgttgccg gacataacgt tccagctcca gttccccggc
     6301 ctgacggtgg atcaaatcac gcaatgtggc gtccaccacg atcaactcat ggatggcagt
     6361 acgcccgcgg aaacctttgt gattacaggc ggggcagccc tgtggatggt acagagtgac
     6421 ggtacgcgcg tcggtaattc ccagcaggcg tttttcttcg tcagtggcag gtgcgggctg
     6481 gcggcagtcg gggcacagcg tgcggaccag tcgctgcgcc atcacacccg tcagactgga
     6541 agagagcagg aaaggctcca cgcccatatc ctgcaaacgc gtaatcgccc ccaccgccgt
     6601 gttggtatgc agtgtggaaa gtaccaggtg ccccgtgagc gacgcctgaa cggcgatttc
     6661 tgcggtttcg gtatcgcgga tttcaccgac catcaccaca tccgggtcct gacgcaaaat
     6721 cgcgcgcagg ccacgggcga aggtcatgcc gacgcgggtg ttaacctgcg tctgaccgat
     6781 cccttcaatc atgtattcga tggggtcttc aaccgtgaga atgttgcgcg aatggttgtt
     6841 cagctcctgc aatccggcgt acagcgtggt gcttttgccg gaacccgtcg gtcccgtcac
     6901 cagaaagatg ccgtgcggtt tgtgcagtag ctggcgcaac tgcgcggtca gttcgtgact
     6961 taaacccaga cgctccagcg tcaggcgggc ctggtttttg tccagcagtc gcagcaccac
     7021 gcgctcgccc caggcagaag gcatggtgga gacgcgcacg tcaatcgccc gaccgcccag
     7081 cagcagcgca atacggccat cctgcggtac gcgcttttcg gcgatatcca gccgtgccat
     7141 caccttaata cgcgatacca gcagcgaggc cagtttgcgc cccggacgca gcatttcatg
     7201 taatgtgccg tcaacgcgaa aacggatcac cagactcttt tcaaacgtct cgatgtggat
     7261 atccgaagcg ccttctttaa tcgcctctgc cagcatggcg ttgatcagtt tgatgatcgg
     7321 cgcatcgtcg tcactttcca gcagatcttc cgtttccggc agttcttctg cgagggtaaa
     7381 aaagtcctcg gcagaaccga gatcttccat caactggcgg gcctcggaag agtcccgctg
     7441 ccagaccgca ttcagccgct gttcaaattc ggcctcgtcg atttgcctca gcgtaaaaga
     7501 cgcgttcagc ccccgctgca gctcctgcaa aacagagagc gacaacggat gaacatggag
     7561 gatctccagc gacgcttcgc accatgccac caggctaaac cgacggctga aactgtaggg
     7621 cagacgcacg gtgttagcgg aggtttcctg tgctacaggc accattaacg cgttctcccg
     7681 gcattgagga acgcgcgaac ttccggcggt aaggcctgat tttgcgctgg tagtaccggc
     7741 tgcgcagtgt tcggcatcag gcttaagcct tgctcatcgc gatagatctg ctcggcgcgc
     7801 atatagttat atttgcgctg cgacacgccg tctgccgcca taccgtcacg cagaatggtc
     7861 ggacggataa acaccatcag gttacgtttt tcttttttat ccgccgtcga tttaaacagg
     7921 ttaccaatca acgggatatc gcccaacaac ggcactttcg ccacgctttc tcccgcctgg
     7981 tcgtccatca gaccgccaag cacgatcagc tcaccatcgt tagccagcac ggtagtcttc
     8041 agtttgcgct cgccaaacac cacatcgagg ctggtctgtc cttccacctt cgacacttcc
     8101 tgctcaatca ccatctgtac cgcgttacct tcgttaatct gcggcgtgac cttcagcatg
     8161 atgccgactt ttttcctttc tactgtattg aaaggattgc tgttattgga gccaacggta
     8221 gagccggtta ataccggaac gtcctggccc accatgaaga aggcttcctg gttgtccagc
     8281 gtggtgatgc tcggcgtgga gagcacgttc gagctggagt cgtttttaac cgcctgtacc
     8341 agtgccatcc agtcgccttt caccacgcca accgccgtac cgctaaagcc ggaaagaagc
     8401 tgggcgagcg tggagagatc gccgttggta tccgggttaa tggtggtagt gccgttttca
     8461 ctgattaccg tcgagccttt ctgcggtttt gcctgagaaa tggctgcacc cagcgtgcca
     8521 atagggatct gcgtaccgtt agcaaactgc attaatccgg catctttcga cgcccactgc
     8581 acgccgaagt tgatattgct gccttcggca acttccacga ttaacgcctc gacatgcacc
     8641 tgagcacggc gaatatccag ttgttcaatc acgctttgca gcgactgcat gatgtcctgc
     8701 ggcgcggtaa caatcagggc attactgtgt ttgctggcgg cgatggagac aacctcacgc
     8761 ccgctaccga ccgtgccttc cgcctcttct ttagccgccg tgagcgtgcc gctgacctgc
     8821 ttcagtacat cgaccagatc ttcggctttg ctgtatttga gatagaaaac ctggctgttg
     8881 ccgctgcgct ccatttctga gtccaggcga cggatcagac ggcgcatttt gtcccgcgtg
     8941 gcggggtcac cactgacaat cacactgttg gtgcgttcat cggcgacaat ttgagatttc
     9001 agcgttgctg gctggttctc gccgctgttt ttggtcaggc tttccagcac gcgggcgatt
     9061 tccgaagccg aggcgttatc cagcgggatc acctcttcag tgcgattacc cgcgtgatcc
     9121 acgcgctgga tcacttctgt cagccgctcc acgacggagg cgcgtccggt aagcataatc
     9181 acgttggagg gatcgtaatt aacaacgttg cctgagcctg cgctgtcgat catctggcgc
     9241 aaaatcggtg ccagttcgcg gaccgacaca ttacgtaccg gcactacttt ggtgaccatt
     9301 tcatcgcccg cgtagttgtc gctgccttca ccgaccaacg gcagtggctc gactttcgcg
     9361 gcgctggatt tcaccacctt cagcacgtcg ttttccatcg gtacgaccgc atacccctgc
     9421 gcttccagca ggttaaggaa tagctggtaa tactggcgtt cattgagtgg agtcatagtg
     9481 cgaatactca ctttcccctg tacgcccggc cccataatga tggttttatt aaggttagcg
     9541 ccgacggttt cgatgaacga tttcaggtcg gtatctttaa aattagcggt gaaagtggct
     9601 tcttccgccc agaccggtga actgcataat gccgctgcca gcaccagcgg cagtaaacgc
     9661 ttttttgttt tgaggccagt tgtcttctta cgccagaccg acaacgtaat atcacgccaa
     9721 aacacgatga atgattctcc tggattaaat gcggttagcg cagcgcgatg gaaatgtcgt
     9781 ggcgcgcacc cttgcgtaaa accgtaagtt gaatggaatc cattgaaggt aactgccgca
     9841 tcagagcaat cattgctcgt ggatcagtga aatcctgctg atttaacgca atggcgatat
     9901 cgccttcctt gaaaccgctg gcatcgaaca gagaacgatc tgcccccggc ttcactgcat
     9961 aaccgacaat cccctcctta cgcacaggcg taagctggat atagttaaaa attttctgcg
    10021 gatctttcgc cagtgcctga cgcacagcag tcgggatctc aactggcgca ctggcagcag
    10081 gctcagcaac agcttgcttt gcttcgtcac tgatggcttt tttgttggtc acggctacgg
    10141 tggaacgctc ctcttctgcc aggctcaggc gctctatttt tccctgatag cgcagcatca
    10201 catggtcgcg gttgatttcc tcaatcaccg cgttgtgaga accaagccgt tccccctgca
    10261 aatagacctg ctgtttgccg ccttcttcaa taaccgcgcc aggtctggta ccaaaggcga
    10321 tcccgcgcag caccacatta agacgtgttt ctgccacagg cgcaggttca ggttgtttta
    10381 ccggcacggc gacaggctga tatttgccaa accagttttg ctggctgatt aattgcacgt
    10441 catttttatc gaacgttttt gcatctgcac ggggcggttt attcgccgat ggggaaaccg
    10501 ccgtatattc cgcagaaaag gagaaatagc gccagagtga atgcgccact tttgcagaaa
    10561 taataagcag catcagccag aacatcgcgc gtacaatttt tcgcagatgc tctttgtctg
    10621 tattaagaga ttgtctctgg ttaagagtgt gacgaatttt tgtcagccat tgaatgagat
    10681 aaattcgtgc gtcacgaaaa acaacccgcg ccaatttata tctacccgac gttatgcttt
    10741 gactattcca caggtggtac gatccagttt ccgctgatac tggttacctg tacgtgtatc
    10801 attaatcgtt atgctgtaat taattccctc ggtaatcatt aatttcaccg tcgggtcagc
    10861 gcacatttgt cgttgatagc tggttaaaaa ggcatcaggc gtctgcgtgg tctgagcacc
    10921 cgcttcgctg ataatggtca tttttaccgt cgtgccgcta ctttgcgcca gcactaaggt
    10981 atatcccgca gatttaatcg gcaggttttg gctgatattt tgcgcctgtt ttttcgccag
    11041 taaactggca ttttcattat gactggcaca gccacttaat aatcctgtaa cgctcaacaa
    11101 tagcgatatt aataccctcc ctggcatttg ttttatcgac atggtaaata atctctaagg
    11161 ttattaataa gagtaaaaat gtcacttcga taatgacgtg gttatcatta aaacaatgcc
    11221 tgtagataaa gtgttgctat accgcccaga cttaaacacg gtccaaaagg cagtgtggtt
    11281 gatcctcttt ttgtaataac ggcatatatc aggccgcagc atgaggcgat taaagcaacg
    11341 ttgggcagtg ataacgcccc cacccaacta cctaacgcag cgaacaataa tacatcgccc
    11401 atgcctaatg cttctttacg cagaactatt ccggctatcc agcgcagaga gtaaaaagtg
    11461 ataaacccca ccaggacgcc ggtaactgca tcttgtagtg ttaacggact ctgttgcgcc
    11521 catgccgcaa tcagcccggt ccacaatacg ccctgagtaa aaacatcggg cagccattgg
    11581 ttgtcgaggt cgatgacgct cgcagcaatc agccaggcgg ataatatcat caccgccagc
    11641 gcccatccac tttccggcca gaccagactc gccagcaaaa aagcgagtgc tgtcaataac
    11701 tcgaccagcg gataacgctt gctgattttc gcctgacaat cacggcagcg ccctttgagc
    11761 atcaaccagg agagcagcgg aatattgtca cgaacgcgga tagtctgctg gcaatgcgga
    11821 cagtgcgagc gtggcagcgc caggcttatt tttgactgcg tactcggcat ttcaccgtga
    11881 aactccgcca tttgttggcg cagcatgatg gggtaacgcc aaatcaccac attcaaaaaa
    11941 ctgccgataa tcaatcctcc gacggtagcc aggacgggca tcgccgcggg gtattgctga
    12001 aaaacatcaa aaaacatggt taaaggttat ttattgtaac ttgccggatg cgggcctgca
    12061 ggtgtatgcc atacggcttt cctccaggcc cgataagctg gcgcaacccg atgcgcctta
    12121 tttcatgccg gatgcggcgc gaacgcctta tccgggttat tcagcaggca tcgaatattc
    12181 ggtaacctta ttaatggttt ccggcccttg cttcggtttc ggcagatcga gtgacgcgag
    12241 cgtgttgtaa gccgactggc tcacaccgcc ttcgaagctc atctccgtcg ctcccggcaa
    12301 ctggtaagca ttcgcgcccg gattccattt cttaaagaac tccgaaagat ccgtctgggc
    12361 gacccaggag gcacacagca tcagcgtgtc ggcagcgtta ccgttagatt cagcacagta
    12421 gttcttactg ccgaacttgt tattgccaac ctcatcgccg cgcgctttac ggtgcatcaa
    12481 ctggaacagg ttccagcctt tcatcccttc acgctcgctg tagaactctg gcagattgcc
    12541 atctggatac cacgtcttga tatcaaagtt tttctctgcc cactctttca gctgtgcgta
    12601 catcagcaga cggtcccccg caccgccgcg cgcccatgcc tgaccgttgc tctcctccag
    12661 atattccggc gcgacggtaa tgtcgtcagc gacacggttc atcttgccga gatagcgatc
    12721 ctgcatgtac agcgccagca cgttgttcgc tacttcagtt gcgccaggaa cagtcagcgg
    12781 cgtttcggcg gcgttgtgac caacttcgtg ccagatcagc cagtcgttca gcggcgtcgt
    12841 cggcagcgtg gtgctgttcg tcgagaagct gctgttcatt accggataac cagagtgcgc
    12901 atcaccgatg gagatctgca catcgttggt gaaacgatgc ttgtgccccg tcaagttttt
    12961 ataggtaaac atccggtgct taccgtcttc atcattacga ccgtagaaat cattcatcga
    13021 gctggcaaag gtatccagat ctttagcgaa ttgtttcagg ccacccttat ccttgtaatt
    13081 gctggcgttc aggttgttct tcggtgcagt gtagacgaaa gaatctgact ccagctcacc
    13141 caacggcgca ggggagttca gagcgttttt ccattcgccg tctttataga acggcgcttt
    13201 taccacacca gtaaaggtga attcggctga ctcattcttt gggctgttgc ccttgatata
    13261 aatcagacca ccgtaaggca ccttgaaggt caccttatca ttggctttca gatcataggt
    13321 tttcgtcact tttggcggac ggttcagcgc gacttcatgc ttctcgcgtc cggtcagatc
    13381 gtcggccagc gccacggtga cagtcacagg aactgatgca gtagactcaa tggtgacctc
    13441 tttctgagcc ggagcccaca ggccagtaga ctgcatgtta cccgcaaacc atttggtcgg
    13501 attcgagtac aggctgatgg tttcagtaac cttctcacct tctgccgata ccgctcccgg
    13561 atacttctcg acatcaactt tgatgttcag atcccaccag gaacgaccca gcatcaggcg
    13621 cgtcagcggt ttttccatat agttgagcgg atagctcggg ttcatcatgc ctgctttgct
    13681 gctaccgtca ccgtagatca tgttgttatc gacaagcgat tttttcagat ctgcagaaca
    13741 ctgcgtgcct tcgctatagg cattgttggc gtagcaattc aggaactcag tgaacgtttt
    13801 aaagcccagc tcgtcatcct tgtcgttttc atagcgatat tcaattttat tccacagcca
    13861 gaccgacatg ttctggtaca gacgttccag atcgacgctg ctcagacgct cacctttgcg
    13921 accattggtc cggaagtaga gctcatgctg atacagacgc tgaatgttgg tgcctaaatc
    13981 cgcagcctgc accatcgctt ttgccgtgtc ggcgttaagg cttagttgcg tatactgtgg
    14041 aacatacatg ccaccagtaa ccggaacccc cgtgccagga cgatattcca gacagttgac
    14101 ctcgtagtgg taagttgggt ccttacactc ctttaatcca ggaaacttct caaagatttt
    14161 tgccttcgca gccttcagag aatcctctgt tttatgatcg gcctcatcaa taaaggcata
    14221 acgcgtttcc tgtttgccat ctacatcttc cagccagctg gcaacttcca gcttcggttt
    14281 gtcatcaggt ttgttttcta cctgatattt ccacttaact tcccctgtct tactatcgat
    14341 ggtgtacggc agcgcaccat ctacggcagg ataacgttca tagacccaaa tgcccgttgc
    14401 gcgctgctga cgaacgcggt tcggataccc ttgcggatcg ttatttacta ccgacttgtt
    14461 cagtgccatc gacagacctg cggcatcaag cagacgtaca aagccagacg cgctctcttc
    14521 cttaagattg ctcatcacgt tttccatgat cagcaccgat ccacctttgt tcagataggc
    14581 gatcagatcg gtcacatcct gctgagtcag cttcggtttg ctggtatctg ctcgcagcgg
    14641 gattgcataa ggatcgttac caacctgagt cacatactca aagccgttga ggatcagcag
    14701 cggcatttcc tgcgggtcga gatcgccata gctacttaaa tgctcaacag agatacccgc
    14761 aaaatccgga tgaaagccga actcagcgct atttcctgta acctgaccat gacgtttgaa
    14821 atagacagta tccaggttgg tgcctacggt catgctggct ttcgcgtccg gagtccattt
    14881 atcgtcggac agatagcgca gtacgttctg catgaagtgc ttcatgtcat ctgaatcacc
    14941 gctgagcgta cactgcccgt ctttattaac gcccccgttc cagctgtaac cgttcggaca
    15001 acgcaaaatg ctgttgtagt gcgggttacc gataaccatc agtttgccct caccgacttg
    15061 ccccagcgaa ataaacggca ggttaaaggt ggcggtatcg cgcgtaacgt tttccggctc
    15121 aacaagagaa ggcgcttccg taatgtacgc cagctcgttt ttatcccagg cgcgtttctc
    15181 gccgaaggcc agccagtagt ttttatcatt acgcgccatc agaatcggga aggccgcgtt
    15241 agagatattc accaccgcct gaccgcgcgc attaccggtg ctgccataga agttggtgga
    15301 gtcatggaat acatggaact tgctgacaga tttgtaactt gtatccacgc cccacagctt
    15361 gttgataacg ccctgaatct ggccgtcatt aacattgcgc gtcgtcagcg agaaccagcg
    15421 tgcctggttg caaccgccag ttttcgcaca gattgcggta tcgatctctt tggcttgacc
    15481 cgtcttaaac tgctcaataa attcgtttgg cagattaaca acttgctcac cttcatccag
    15541 cgtcgcaccg ttggataacg agagattgat aatctcgttg atcacgttgg gatattcggc
    15601 aaagaccttg cgtacatcgt ccggaacaac acgggtatta ttttgcccgg tcgtcgaata
    15661 gcgatgaata agctgatcaa tattcgcccc gcgaacttca tcacccagtt cagtcaatgc
    15721 aatggtcgac ttattgccgc gcactgagcc cagttcaaag gtatcgatac caaaggagat
    15781 ggtttcgccc cagctaaagg aaaattcacc attttcccct gtcacgccac ggcctgaatt
    15841 ggtgtagtag ttgacgccag caacaccata tccctggcta tcgaccagtc ggccttcgga
    15901 gagaatgatt tccgaaggtt gatactgata aaactgttcc gcgttagccg acacgaagga
    15961 agcgttcaga tccggttttg ttcccggcgt ggtgacaggc acaaccggtg aggtatgagt
    16021 ggatggcgct ttatcggtcg cagcattgtt ttccacctct tcattgacca gctttttgaa
    16081 ctcttccggt gccagatcga tttgcttata cagtgagtcg aaacgtttac tctcgatcac
    16141 agaggagaac gtcaggcaaa cctgttctgt attcgccgga cagctgttac tggacgttac
    16201 cagcgaaacc gcattgcttt tcttgtcatc ggaagccgcc agttcttgcg catcctcaag
    16261 actaaacgac actttttcaa ccgcacgcag gctacgcgca gcttctgact gggtgtcgaa
    16321 ggtagcaatt gtcgtgttgt tccctgccac acaggtgact ttgtcgcctg gcgtaaaggt
    16381 aaagccatcg ctggattcac cattacaagt agcaccagtt atccgctggc ttccgcccag
    16441 ggtcagataa cccgttttcg taggaacagg ttctggctcc ggttctggtg ttggctcagg
    16501 atcaggtgtc ggttccggcg taggttctgg gtccggcgtt ggctcaggcg tcggctccgg
    16561 gtttggtgtt ggatcaggtt tcacttccgg caaagaccct gttccagaat ctacaggcgg
    16621 cgtatcggag gaagatccgg aaccaccgcc atcacaaccg gctaacaggg ttgcgctcaa
    16681 aatagccgct aaaagcgatt tcttatattt aaatttctta
//
LOCUS       DACBVI010000097        16497 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.097, whole genome shotgun sequence.
ACCESSION   DACBVI010000097 DACBVI010000000
VERSION     DACBVI010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.097"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            141..788
                     /gene="ychE"
                     /locus_tag="IU325_15415"
     CDS             141..788
                     /gene="ychE"
                     /locus_tag="IU325_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HAP5810394.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(946..1242)
                     /locus_tag="IU325_15420"
     CDS             complement(946..1242)
                     /locus_tag="IU325_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810395.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLLIQSNRDWL
                     SMSLFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            1526..3157
                     /gene="oppA"
                     /locus_tag="IU325_15425"
     CDS             1526..3157
                     /gene="oppA"
                     /locus_tag="IU325_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HAP5810396.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKATDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            3243..4163
                     /gene="oppB"
                     /locus_tag="IU325_15430"
     CDS             3243..4163
                     /gene="oppB"
                     /locus_tag="IU325_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HAP5810397.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            4178..5086
                     /gene="oppC"
                     /locus_tag="IU325_15435"
     CDS             4178..5086
                     /gene="oppC"
                     /locus_tag="IU325_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HAP5810398.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            5098..6111
                     /gene="oppD"
                     /locus_tag="IU325_15440"
     CDS             5098..6111
                     /gene="oppD"
                     /locus_tag="IU325_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HAP5810399.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSSPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            6108..7112
                     /gene="oppF"
                     /locus_tag="IU325_15445"
     CDS             6108..7112
                     /gene="oppF"
                     /locus_tag="IU325_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HAP5810400.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGNYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTFQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(7165..7494)
                     /locus_tag="IU325_15450"
     CDS             complement(7165..7494)
                     /locus_tag="IU325_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF440 family protein"
                     /protein_id="HAP5810401.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(7529..8989)
                     /gene="cls"
                     /locus_tag="IU325_15455"
     CDS             complement(7529..8989)
                     /gene="cls"
                     /locus_tag="IU325_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP5810402.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            9132..9305
                     /locus_tag="IU325_15460"
     CDS             9132..9305
                     /locus_tag="IU325_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HAP5810403.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(9360..10613)
                     /locus_tag="IU325_15465"
     CDS             complement(9360..10613)
                     /locus_tag="IU325_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HAP5810404.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(10914..11210)
                     /locus_tag="IU325_15470"
     CDS             complement(10914..11210)
                     /locus_tag="IU325_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HAP5810405.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            11434..12153
                     /gene="tonB"
                     /locus_tag="IU325_15475"
     CDS             11434..12153
                     /gene="tonB"
                     /locus_tag="IU325_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HAP5810406.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(12193..12591)
                     /gene="yciA"
                     /locus_tag="IU325_15480"
     CDS             complement(12193..12591)
                     /gene="yciA"
                     /locus_tag="IU325_15480"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HAP5810407.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(12696..13235)
                     /locus_tag="IU325_15485"
     CDS             complement(12696..13235)
                     /locus_tag="IU325_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HAP5810408.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATALVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(13265..14008)
                     /locus_tag="IU325_15490"
     CDS             complement(13265..14008)
                     /locus_tag="IU325_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HAP5810409.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSTLRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(14036..14125)
                     /locus_tag="IU325_15495"
                     /pseudo
     CDS             complement(14036..14125)
                     /locus_tag="IU325_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            14365..15003
                     /gene="ompW"
                     /locus_tag="IU325_15500"
     CDS             14365..15003
                     /gene="ompW"
                     /locus_tag="IU325_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HAP5810410.1"
                     /translation="MKKLTVAALAVTTLLSGSAYAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(15063..15569)
                     /locus_tag="IU325_15505"
     CDS             complement(15063..15569)
                     /locus_tag="IU325_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HAP5810411.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPTIEAILNEEKEMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(15615..16115)
                     /locus_tag="IU325_15510"
     CDS             complement(15615..16115)
                     /locus_tag="IU325_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HAP5810412.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(16201..16380)
                     /locus_tag="IU325_15515"
     CDS             complement(16201..16380)
                     /locus_tag="IU325_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810413.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
BASE COUNT         4354 a         3838 c         4001 g         4304 t
ORIGIN      
        1 cacaaccaga taacaataac gaatgatagc aattttaagt agttaggagg tgaaaaatgc
       61 tgtcaaaagg cgtattgtca gcgcgtcttt ttaaccttat ttatggctaa cattatccgg
      121 cttttgcttc ggagctaacc gtgattcaga ccctttttga ttttcccgtt tacttcaaat
      181 ttttcatcgg gttatttgcg ctggtcaacc cggtagggat tattcccgtc tttatcagca
      241 tgaccagtta tcagacagcg gcagcgcgaa acaaaactaa ccttacagcc aacctgtctg
      301 tggccattat cttgtggatc tcgctttttc tcggcgacac gattctgcaa ctttttggta
      361 tatcaattga ttcgttccgt atcgccgggg gcatcctggt ggtgacgata gcgatgtcga
      421 tgatcagcgg caagcttggc gaggataaac agaacaagca agaaaaatca gaaaccgcgg
      481 tacgtgaaag cattggtgtg gtgccactgg cgttgccgtt gatggcgggg ccaggggcga
      541 tcagttctac catcgtctgg ggtacgcgtt atcacagcat tagctatctg tttggtttct
      601 ttgtggctat tgcattgttc gctttatgtt gctggggatt gttccgcatg gcaccgtggc
      661 tggtacgggt tttacgccaa accggcatca acgtgatcac gcgtattatg gggctattgc
      721 tgatggcatt ggggattgaa tttatcgtta ctggtattaa ggggattttc cccggcctgc
      781 ttaattaatt cctttcaaat gaaacggagc tgtcatgctc cgtttacttc gtcattattt
      841 ttactttgtt cccgcgcagt tatcaaaagc aaaaggaatg ggtgaaaata ttcttctcaa
      901 attacagttg gttataagga tttccttaac tgcttctcct caccatcatg ttattttcgc
      961 cacatcataa tcctgggctt gctgaagaat aattgaaatg atattagtaa ttccactgcc
     1021 tttggtagag gaaagtgcta aataataatc aattgttaaa ttattgtgca tttcactact
     1081 ggaactgtaa tcagaaaaga gagacatgct taaccaatct ctatttgatt gaattaaaag
     1141 atgtttgtta agacatggat gcaagctata gattctgata cggtcaataa aagagaattg
     1201 cttaacaatt ttgcaaaatg tattggcgag taagaaccgc atttggtact ttccgggcaa
     1261 ccgccagacg attctttatt ggtaatgaga ataattaaca attaaagagc gtcgcgaaag
     1321 aataatgtgt ctcgacaggg gagacacagt acgaatcgac ataaggtgat cgtctgaatc
     1381 accagaataa ataaagtcgg tgatagtaat acgtaacgat aaagtaacct gacagcagaa
     1441 agtctccgag cctgtgcagg gtcccaatcc gggattacac atgctggtta ataccagtaa
     1501 ttataatgag ggagtccaaa aaacaatgac caacatcacc aagagaagtt tagtagcagc
     1561 tggcgttctg gctgcgctta tggcagggaa tgtcgcgctg gcagctgatg tacccgcagg
     1621 cgtcacactg gcggaaaaac aaacgctggt acgtaacaat ggttcagaag ttcagtcatt
     1681 agatccgcac aaaattgaag gtgttccgga gtctaatatc agccgagacc tgtttgaagg
     1741 cttactggtc agcgatcttg acggccatcc ggcacctggc gtcgctgaat cctgggataa
     1801 taaagacgcg aaagtctgga ccttccattt gcgtaaagat gcgaaatggt ctgatggcac
     1861 gccagtcaca gcccaagact ttgtgtatag ctggcaacgt tctgttgatc cgaacaccgc
     1921 ttctccgtat gccagttatc tgcaatatgg gcatatcgcc ggtattgatg aaattcttga
     1981 agggaaaaaa ccgattaccg atctcggcgt gaaagctatt gatgatcaca cattagaagt
     2041 caccttaagt gaacccgttc cgtacttcta taaattactt gttcacccgt caacttcacc
     2101 ggtgccaaaa gccgctatcg agaaattcgg cgaaaaatgg acccagcctg gtaatatcgt
     2161 caccaacggt gcctatacct taaaagattg ggtcgtaaac gaacgaatcg ttcttgaacg
     2221 cagcccgacc tactggaaca acgcgaaaac cgttattaac caggtaacct atttgcctat
     2281 tgcttctgaa gttaccgatg tcaatcgcta ccgtagtggt gaaatcgaca tgacttataa
     2341 caacatgccg atcgaattgt tccagaagct gaaaaaagag atcccggacg aagttcacgt
     2401 tgatccatac ctgtgcactt attattacga aattaacaac cagaaaccgc catttaacga
     2461 tgtacgtgtg cgtaccgcac tgaaactggg tatggaccgc gatatcattg ttaataaagt
     2521 gaaagcgcag ggcgacatgc ccgcctatgg ttacactcca ccgtatactg atggcgcaaa
     2581 attgactcag ccggaatggt ttggctggag ccaggaaaaa cgtaacgaag aagcaaaaaa
     2641 actgctggct gaagcgggtt ataccgcaga caaaccgttg accatcaacc tgttgtataa
     2701 cacctccgat ctgcataaaa agctggcgat tgcagcctct tcattgtgga agaaaaacat
     2761 tggtgtaaac gtcaaactgg ttaaccagga gtggaaaacg ttcctcgaca cccgtcacca
     2821 gggcactttt gatgtggccc gtgcaggctg gtgtgcagac tacaacgaac caacttcctt
     2881 cctgaacacc atgctgtcga acagctcgat gaataccgcg cattataaga gcccggcctt
     2941 tgacagcatt atggcggaaa cgctgaaagc gactgacgag gcgcagcgca cagctctgta
     3001 cactaaagca gaacaacagc tggataagga ttcggccatt gttcctgttt attactacgt
     3061 gaatgcgcgt ctggtgaaac cgtgggttgg tggctatacc ggcaaagatc cgctggataa
     3121 tacctacacc cggaatatgt acattgtgaa gcactaatgg caatacgtgg ggcaggcgtg
     3181 tcctgctcca cggtgtctga tttttatcgc attacagaag gcacaggcca gaaggtaggg
     3241 caatgttaaa atttattcta cgtcgctgtc tggaagcgat tccgacgcta tttattctta
     3301 ttactatttc gttcttcatg atgcgcctcg cgccgggaag cccttttacc ggcgaacgta
     3361 ctttaccgcc agaagtgatg gccaatatcg aagcgaaata tcatcttaat gatccgatca
     3421 tgacacagta tttcagctac ctgaaacaac tggcgcatgg cgatttcggt ccatcgttta
     3481 aatataaaga ttattcggtc aacgacctgg ttgcatccag ttttcccgtt tctgccaaac
     3541 tgggagccgc agcatttttc cttgcggtaa tactgggtgt tagtgctggc gttattgccg
     3601 cattaaaaca aaacaccaaa tgggactata ccgtgatggg gctggcaatg accggggttg
     3661 ttatccctag ttttgtggtt gcgccattat tagtcatgat atttgcgatc attttgcatt
     3721 ggctgccggg cggtggctgg aatggtgggg cgcttaaatt catgatattg ccgatggtgg
     3781 cgttgtcact cgcctatatc gccagtattg cgcgtattac ccgtggctct atgattgagg
     3841 ttttacactc caactttatt cgtactgccc gggcgaaagg gttacctatg cggcggatca
     3901 ttttacgcca cgcattaaaa cctgctctgt tacccgtgct ctcctatatg ggccccgcat
     3961 ttgtcggcat tattaccggt tctatggtta tcgaaaccat ttatggtttg ccggggattg
     4021 ggcaactgtt cgttaatggc gcattgaacc gtgactattc cttagtgtta agcctgacta
     4081 tcctggttgg tgcgttaacc attttgttta atgccattgt cgatgtgcta tatgcggtta
     4141 tcgacccgaa aatccgttac tgatactgga gctcgcgatg atgttaagta agaaaaacag
     4201 cgagacgctg gaaaatttca gtgaaaagct ggaggtcgaa gggcgcagct tgtggcagga
     4261 cgcacgtcga cgttttatgc ataaccgtgc ggcggttgcc agtctgatag tgctggtgct
     4321 gatcgcgtta tttgtaatcc tggcaccgat gctttcgcag tttgcctatg acgatactga
     4381 ctgggcgatg atgtccagcg ccccggatat ggagtccggt cactactttg gtactgactc
     4441 atccggtcgc gacctgcttg tacgcgttgc gattggcggg cgtatctcac tcatggtcgg
     4501 tgttgctgcg gcactggtgg ctgtggtcgt cgggacactt tatggttcgc tttccggtta
     4561 tctgggcggt aaagtggatt cggtaatgat gcgtctgctg gaaatcctca actccttccc
     4621 attcatgttc ttcgtcattt tgctggtgac ctttttcggt caaaacatcc tgctgatttt
     4681 cgtggcgata ggcatggttt cctggctgga tatggctcgt attgtgcgtg ggcaaaccct
     4741 gagtctgaag cgcaaagagt ttattgaggc ggcacaagtt ggcggtgtat cgacgtcggg
     4801 cattgttatt cgccacattg tgccgaatgt actcggtgtg gtggtggtct acgcatcact
     4861 tctggtgccc agcatgatcc tctttgaatc tttccttagc ttcctggggt tgggtacgca
     4921 agagccgtta agcagctggg gtgcattgct gagtgatggc gcgaactcga tggaagtctc
     4981 tccatggtta ctgttgttcc cagcgggatt cctcgtggtg acactgtttt gtttcaactt
     5041 tatcggcgat ggcttgcgtg atgccctcga cccgaaagat cgttaaggag tgcagccatg
     5101 agcgtaattg aaactgcaac tgtgccgctc gcacaacaac aggctgacgc actgctgaac
     5161 gtgaaagatt tgcgtgtcac ctttagttcc ccggacggcg acgtcacggc ggtcaatgat
     5221 ttgaattttt ccctacgtgc cggagaaacg ctgggcattg taggtgagtc tggttcgggt
     5281 aaatcgcaaa ctgcatttgc gttgatgggc ctgttggctg ccaacggacg tattggcgga
     5341 tcggcaacct tcaatgggcg tgaaatcctc aatttgccag agcatgaact caataaactg
     5401 cgcgctgaac aaatctcaat gatttttcag gacccaatga cttcgttgaa tccctatatg
     5461 cgtgtcggtg agcagttgat ggaagtgctg atgctgcata agaacatgag caaagctgag
     5521 gcgtttgaag agtcggtgcg gatgctcgat gcggtaaaaa tgccggaagc gcgtaaacgc
     5581 atgaaaatgt acccgcacga attttctggc ggcatgcgtc agcgagtcat gattgcgatg
     5641 gcattgctat gtcgacctaa gctgctgatt gcggatgaac ccactactgc gctggacgtc
     5701 accgtacagg cacagatcat gacgctatta aatgaactga agcgggaatt taataccgcc
     5761 atcattatga tcacccacga tcttggcgtg gtggcgggga tctgcgacaa agtgctggta
     5821 atgtacgccg ggcgcacgat ggaatatggc aatgcgcgcg atgtctttta tcaacccgtt
     5881 catccttatt ctatcggttt gctcaacgcg gtgccgcgtc ttgatgcgga aggtgaaaca
     5941 atgttgacca tcccgggtaa tccgccaaac ttgctgcgat taccgaaagg ttgcccgttc
     6001 cagccacgtt gtccgcatgc gatggaaatt tgtagtagcg caccgccgct ggaagagttt
     6061 acgcctggcc gtctgcgtgc ttgctttaaa ccggtggagg aactgttatg aatgctgtaa
     6121 ctgaaggaag aaaagtcctc cttgaaatcg ccgatctgaa agtgcacttt gaaatcaaag
     6181 atggcaaaca gtggttctgg caaccgccga aaacgctcaa agccgtcgat ggtgtcactc
     6241 ttcgcctgta tgaaggggaa actttaggtg tggtagggga atcgggatgc ggtaagtcca
     6301 cctttgctcg cgccatcatc ggtttggtca aggcgaccga cggtcatgtt gcctggttag
     6361 gtaaagagtt gctgggcatg aagcccgatg aatggcgtgc cgttcgcagt gatattcaga
     6421 tgattttcca ggatccgttg gcatcgctta acccgcgtat gaccatcggc gagatcatcg
     6481 ctgaaccact gcgtacttat catccgaaaa tgtcacgcca ggaagttcgc gagcgcgtga
     6541 aggcgatgat gctgaaagtc gggttattgc ctaacctgat taaccgctat ccgcatgagt
     6601 tctctggtgg gcagtgccag cgtatcggga ttgctcgtgc tcttattctt gaaccgaagc
     6661 tgattatctg tgatgagccg gtgtcggcgc tggacgtgtc aattcaggcg caggtggtca
     6721 acctgctcca gcagctgcaa cgcgagatgg gattgtcatt aatttttatc gctcacgacc
     6781 tggccgtggt aaaacacatt tccgatcgtg tgctggtgat gtatctcggc catgcggtag
     6841 aactggggaa ctatgatgag gtctaccaca atccactaca tccttacacc cgggcattga
     6901 tgtcggcagt ccccatacct gatccggatc tggagaagaa caaaaccttc cagttactgg
     6961 aaggggaatt accgtcgccg atcaacccgc cttccggttg tgttttccgt acccgttgcc
     7021 cgattgccgg tccggagtgc gccaaaacac gtcctgttct ggaggggagt ttcagacacg
     7081 ccgtttcttg cctgaaagtc gatccgcttt aaacacaata agggctgaca gttgtcagcc
     7141 ctttttcacg ccgaaagcga ttatttattc ccgccagatg atatggcaca gtttgtgatc
     7201 tttttcgcgg cataacagaa tgcgggcgaa aacatcgttg atttcaccat cttcactgtc
     7261 cgccagacca atcaccactt cggcaaaaaa gtccgggttc aaatcgaaat caacatgttc
     7321 ctgccagtct tctgccggat cgaataactc cgcgccgccg cgctcttcaa actgaagatt
     7381 gaacagcaga acatcagctg gatcgagatt gtcggcagcc agttcgagaa aaatatcgta
     7441 ggcctgctca agcgtttcat cttcagtcag gcgattgttt agatccatat ccatagtcac
     7501 tacctgttta acctctgttg gcgacgtttt acagcaacgg gctgaagaag taaaacagtc
     7561 gctcggcgac acgttgccat aatggacgtt ttagccataa acgggcatcg agcagacgag
     7621 aacgcgaaat ataatcgtcc tgaacggcgg cgaggtcagc accaaaccct ttatcgtcga
     7681 ttgccagggt aatctcgaaa ttcagccaca gactacgcat atcaaggtta acggtaccaa
     7741 ccagacttaa ttcgccatcg accagcacgc tcttggtatg caataacccg ccttcaaact
     7801 gataaatttt aaccccagca gccagcagtt ccgtaaagaa cgcgcgactg gcccagccaa
     7861 ccagcattga atcatttttt cgcggaagga taatactgac atccaccccg cgctgcgccg
     7921 ccgtgcaaat cgcatgaagt aaatcatcgc tgggcacaaa gtagggcgtc gtcatgatca
     7981 aatattcacg tgccgaataa gccgcagtca gtaatgcctg gtgaatgaga tcttccggaa
     8041 agccggggcc agaagcaatt gtgtgaatgg tgtgaccgct ggcctgctca aacggcataa
     8101 tattgacatc tggtggtggc ggcagaatac gtttcccggt ttcaatttcc cagtcgcagg
     8161 aataaataat ccccatcgcg gtagcgatgg gaccttccat acgcgccatc agatcaatcc
     8221 attgccctac gcccgcatct tgtttgaagt agcgagggtc gaccatattc atgctgccgg
     8281 tgtacgcgat gtaattatcg atcatgatca tcttgcgatg ttggcgcagg tccatacggc
     8341 gtaaaaacac acgcatcaga ttgaccttta aggcttcgac cacttcaata ccggcattac
     8401 gcattagctc gggccagggg ctgcggaaaa aagccacact cccggcggag tcgagcatca
     8461 atcggcagtg aatgccgcgt cgcgcagcgg ccattaatga ttcagccacc tggtccgcca
     8521 tgccgccggg ctgccagata taaaacacca tctcaatatt atggcgcgcg agctggatgt
     8581 cgcggattaa cgcctgcatc acatcatctg actcggtcat cagttgtagc tgattccctt
     8641 tgacccctgc gatcccctga cgacgctcgc aaagcttgaa taatggcgca gcgacactgc
     8701 tattttcttc ggcgaagata tgcttacagg ctttaaggtc gttaagccat tttgcggtgg
     8761 aaggccacat cgctctggcg cgctcagcgc ggcgtttgcc taaatggagc tcgccaacgg
     8821 caagataggc aataattccg actaacggca gaatgtaaat aatcaacagc caggccatcg
     8881 cggagggaac tgcgcgtcgt ttcattagaa tgcgtaaagt tacgcctgca atgagcaacc
     8941 agtatcccag aatggccaac caactcacca acgtataaac ggttgtcata gattagaaat
     9001 ccttttgaaa gcgcattgtt atgagtttac gcatatggat tcatctggca aataaaaacg
     9061 cgggaaaagc actggtcagc gtcgggggcg ggggtataat gaccattctg ttattgcata
     9121 gagtagttaa catgaagcgg agtagaacgg aagtggggcg ctggcgcatg cagcgtcagg
     9181 ctagccgacg taaatcgcgt tggcttgagg ggcaatcgcg ccgaaatatg cgtatccaca
     9241 gcatcaggaa gtgcattcta aacaaacagc gtaactcgtt attgtttgcg atctacaata
     9301 tctaaatgta ctgggcacca ttgcggtgcc caataagttt actcttccgt ctttaaactc
     9361 tatttttgcg ccgattcttt actgtcagct ttcgtttctt gttcatcgtt atcgctgaaa
     9421 atgccatgac cggagttcaa caacattgaa acgagcatat cattattaat ctcttcacca
     9481 tttaaaactc gcgccagaat ttcgctgcca aacagttgcg gcgagagaat gatatccgga
     9541 tgtaccatct taatcttatt caggtttttg ctatcactga cggcgagaac tgttttgaca
     9601 tcactgctca tatcttttgc cgagagtaca acaaacgcgt tatctgcatc gttatcactc
     9661 agcgccagaa tggcccggca acgatcgatt cccgctttct tcaagactga actgtcatta
     9721 ctgtcaccgg ggataacatc agcgttatcg cctaagcgtt gctcaagttg cttgatatca
     9781 tcttcaggca agttgctgat aaccgttacg ttttgtccgc gttgattcag ttgcaggatc
     9841 gtattgatgg cgagaatcga atgtccgcaa acaataaaat gatctttacg atgcattgta
     9901 tgattgtttc cttttacaag tttgttgaat cccccgcgga taagcggacc aaaaattgag
     9961 gtcatagagg tggcaaaaac ggtaatgccg gaaataatga ccgaaatagt gaacaatcgt
    10021 gctgattcag aaacagggac aatatcgcca tagccgacgg ttgacatggt ttctatcgaa
    10081 aaatagaacg cggtcatcaa actttctatt cgcggattaa aaccttcgct taaataaagc
    10141 gcaccgtagg tggagtaaaa cagtaacgtc gtgaaactaa taaatgcgaa aattgtcccg
    10201 gctgcggcgc tactgtggga gaagtctttg cgcagtatca gcaaaaacac cagcgtaaaa
    10261 atgcaaaatc caatactaaa tttcagccag ggataaaaat gcagggtgta aattagcgct
    10321 atcaacaaca aaatgatact gattgcccat gcgagctttg cccggaacag taagccaatg
    10381 gagttcagga caagaaagac gccgagcata aagagtgggg cgtttgccag caaggagaga
    10441 tccaactctg aaaatgcttt gatatggaaa atatctagca aattgaccga cataccatag
    10501 attgttttaa aaataagcaa tccatttaaa aagacggcca gcgcgagaat gtcgtgccgt
    10561 agggtcaccc ataaattagt tgctgtttgt ttgaatgtag cccagtgact cactgatacc
    10621 tctgtaacca caattttgtt taattatctg agcgtgcaca ttttctgaac atacatgcag
    10681 cgcgtgaatg tgttaaacaa tagtagatga ctttatccgc agtaatcaaa ggttttgagt
    10741 gagcaactct gcgtcaggaa agggcatttt tcctgaatat gcttacatca tataaagaaa
    10801 tacagataaa gttattatct gcttgtgggg gtgaatgcac tgaccgacct ataaggaaag
    10861 gccaaacaag aacacggttg caaaaaccgt gcccttaaat attgaatctc tattcagaac
    10921 actttcttaa atggtttcac tgaaacgtgt tcatagactc ctgccgctac gtacgggtca
    10981 gcatcggccc aggcctgagc tgcttccagc gattcaaatt cagcaataac ggttgagcca
    11041 gtaaatcccg cagcccctgg atcgttactg tctaccgctg gcattggacc agctgtcaac
    11101 aaacgacctt catcatgcag taactgtaaa cgtgctaaat gtgccggacg aacggaaagg
    11161 cgtttttcga ggctatcagc tttgtcttga gcgtaaataa catacaacac gggcacaact
    11221 ccttgttcgg gaaagttgta aagtacgtta tttgaaaggg cgaagatctg caacggaaag
    11281 atgatgtctt tgttaagacc atgcataaag taaggtcaat tacgccaaaa atgacatttt
    11341 cactgatcct gatcgtcttg ccttattgaa tatgattgct atttgcattt aaaatcgagg
    11401 cctggttttt ctactgaaat gattatgact tcaatgaccc ttgatttacc tcgccgcttc
    11461 ccctggccga cgttactttc ggtctgcatt catggtgctg ttgtggcggg tctgctctat
    11521 acctcggtac atcaggttat tgaactacct gcgcctgcgc agccaatttc tgtcacgatg
    11581 gttgcgcctg cggatctcga accgccacaa gccgttcagc cgccaccgga gccggtggta
    11641 gagccagaac cggaacctga gccgatcccc gaaccgccaa aagaagcacc ggtggtcatt
    11701 gaaaagccga agccgaaacc taagccaaaa ccgaagccgg tgaaaaaggt acaggaacag
    11761 ccaaaacgcg atgtcaaacc cgtagagtcg cgtccggcat caccgtttga aaatacggcg
    11821 cccgcgcgcc cgacatcaag tacggcgacg gctgcaacca gcaagccggt taccagtgtg
    11881 gcttcaggac cacgcgcatt aagccgtaat cagccgcagt atccggcacg agcacaggca
    11941 ttgcgcattg aagggcaggt taaagttaaa tttgacgtca cgccggatgg tcgcgtggat
    12001 aacgtacaaa tcctctcagc caagcctgcg aatatgtttg agcgtgaggt gaaaaatgcg
    12061 atgcgcagat ggcgttatga gccgggtaag ccaggcagtg ggattgtggt gaatatcctg
    12121 tttaaaatta acggcaccac cgaaattcag taagcataaa gtcaaaagcc tccgaccgga
    12181 ggcttttgat tattactcaa caggtaaggc gcgaggtttt ccttcaggat caaccgcgac
    12241 atacttaaat aatgcttctg tcgctttata gcgttgccca atgggttcag acgcgacttt
    12301 tttcacccac acttcaatat taatgctaac cgatgtcgtc cctttctgga cacagcgtgc
    12361 atagcagcac accacatcgc cgaccgcaac cggtcgtaag aaagtcattc cttcaacccg
    12421 cacggtcact acgcgaccgt gggcaatttc tttcgccaga atagcgccgc caatatccat
    12481 ttgtgacatt aaccaaccac caaagatgtc accattggca ttggtatcgg cgggcatggc
    12541 taaagtacgt aaaacaagat cgccctgagg gacgttatgt gttgtagaca tggtaaaacc
    12601 gacttaaaag gaaaatcaca ggcgcgatgc tactatgatt ttcctgagga gaacagagga
    12661 gatacagcgc cagccccgaa ggactggcag tctggttagg atttatcttc ctgcggcatg
    12721 tggcggtaga tatagatacc gcttaacaat gtgaagatta aggtaagggc ggtcaggcca
    12781 aagactttaa agttgaccca aatattttgc ggcagccaga atgcgatgta gatgtttgcc
    12841 agaccgcaaa ggataaagaa aacagcccag gccagattca gcttcgacca taccggttgc
    12901 ggcagcgtga gttctttacc cagcatccgc tgaattagcg gctttttcat cacccattgg
    12961 ctgactaaca gggcacccgc aaacagggca taaatgacag taaccttcca tttaataaac
    13021 tcatcattgt ggaagaacag ggtcaagcca ccgaagacga ccaccagaac aaaagtgatc
    13081 agggccatct tctcaacctt acgaaagcga acccagctat atataagcac aagcgccgtg
    13141 gcgacgatca gcgccgcagt agccgcatag atgtcataaa tcttgtaaaa cgcgaaaaag
    13201 acaaccagcg gtaaaaaatc aagaaactgc ttcattttac gattccgtaa tcaaatgctt
    13261 aaggttattg gcgaatcaac atatacaggc ggaacagata gatgagcagt atggctgaaa
    13321 tcaggttgct caaggtgttc gcaagtacgg caccaatttc cggggttaat gcggcaaaag
    13381 aagaggcaaa aagcagcaac agtgtttttg ccagcaacca gctcagtact gcgggtgcca
    13441 ccagacgcat attcgcccaa gtcagccgca tactgctacg catcgaggca aaaacgccca
    13501 ttttgtcctg taccaacatc accggtgcca gggccagtaa aatggccatg ataattcccg
    13561 gaacgaccac cagcatgatg ccaatctgta ctaaaagggt agtcagaaaa atcagaataa
    13621 ataactttgg caatatcggc gcactggcac caatagctcg cagcgtactg actctctgac
    13681 ccgcagacac cagctggata attaatatta cgcctccggc gagaatggcg ttaccgatta
    13741 atcctgaaaa agtggacgcc gctgaagcct gcagcaaaat ttgttgctgt tccggtgaca
    13801 tattctgaac caggtcgaac aacccactac tgccgctaac gggcacgccg tcattgagct
    13861 gcgcaagctg tgcatcactg ggtgagaaaa catgccctaa caccactgtg ataaacgcac
    13921 atagcaacga taccaacaga atggtcataa attgattacg gaagaaattt ccggtgtcac
    13981 ggtatacgga ctgcgccgtg atagacatgc actctccttg agttttatgc aggtgttaat
    14041 tagcgggcaa ttgtaccctg gttaagcctc gggtggcagc atcaggtctt gtatggaaaa
    14101 gcatatcttt gtaaagcgga ggtaattgct ggccttcagg cgtgggcaaa ccgcggcaag
    14161 tggcgaacga gttaatattt gcgtagcgaa aatatttaaa aattgattta aatcacatta
    14221 accaggattc tcaatgcaac ttctaaatta atccagatca ataaatggtg aattatcata
    14281 tgtaatgtga tctatgtagg atcatttgtt actccaatgt aggtatattc gtcacgtttt
    14341 tataaccata acgacggagc ggatatgaaa aagttaacag tggcggcttt ggcagtaaca
    14401 actcttctct ctggcagtgc ctatgcgcat gaagcaggcg aattttttat gcgtgcaggg
    14461 tctgcaaccg tacgtccaac agaaggtgct ggtggtacgt taggaagtct gggtggattc
    14521 agcgtgacca ataacacgca actgggcctg acgtttactt atatggcgac cgataacatt
    14581 ggtgtggaat tgctggcagc gacgccgttc cgccataaaa tcggcacccg ggcgaccggc
    14641 gatattgcaa ccgttcatca tctgccgcca acactgatgg cgcagtggta ttttggtgat
    14701 gccagcagca aattccgtcc ttacgttggt gcaggtatta actacaccac cttctttgat
    14761 aatggattta acgatcatgg caaagaggcg gggctttccg atctcagtct taaagactcc
    14821 tggggagctg ccgggcaggt gggggttgat tatctgatta accgtgactg gttggttaac
    14881 atgtcagtgt ggtacatgga tattgatacc accgccaatt ataaactggg cggtgcacag
    14941 caacacgata gcgtacgcct cgatccgtgg gtgtttatgt tctcagcagg atatcgtttt
    15001 taattccgca caaaaacgac cccgtaatat acggggtcaa taaggacatg gtataaaggg
    15061 tattatttct tcgcttctac gccatcagtt tcagagcgaa ttaaaaattt ctcagttgtt
    15121 tgcggaatat gctgaatcag ccagtcggcc atttcttttt cctcatttaa aatcgcttcg
    15181 atggttggaa ttgaagctgt atcaccggca ttttttgctg ctgctaatag tgaggtgtaa
    15241 caggcgattt caaattgctc gaagacatat ccgctaatag agcctttgac tatttcatca
    15301 gaagggaata taccaccgat tgactgccca agcgcagcca ttttactcat ggaatcttta
    15361 atgactgaac gtgaaatgtc attacgatca agaatagttt ccagttgaac aatctggttt
    15421 ttggtttcac taagatgttg ttcaatacga gcgcgtagtt caggataatt atctatacga
    15481 ctagccatgg attcaagcat agattcggct tgcttttcca ttgcgtgggc gtcacgtaac
    15541 cagtcatgat aatgttcaat acgattcatg aaaatgtcct ctttataaaa tgactaaaag
    15601 ttaaagttca tatttcaggc tttattttcg gctttcttat ttacgttatt aagtgccaga
    15661 tcagtcagtt tgatgtcggt ggccttttct tcttcaagag tttctttcag aagcttcgct
    15721 gctttacggt aacctaattg ttcagccagc gtcgctaatg tcccgtaact ggcgatctca
    15781 tagtgctcga ctttctgcgc tgcggcaatc agtgcggcat cacgcacttc gtttttctcg
    15841 gtgctttcga taacctcatt agcttcttca atgagacctt ccattgccac acatttcatg
    15901 cgcttaattt tcaggttcga ttcagattcc acaacttgat caatacgttc aatctgtcca
    15961 tgagtttcct cgaggtgcgc atgaaaagcc tgacttaatt tttcgtttga tgttgctctt
    16021 gcgagttttg ccagtgcccg ggttaattgt ttttctgcgc tgtaggtatc tgaaagcagg
    16081 tgaataaata catcttcaat ggtcttcata ttcatatttt tctccagtga aatcactgcg
    16141 agccatacgg cccgcagatg caattatctt tatcagctga tattaataat taacggtgaa
    16201 tcaggatttg cctgatttat taccaccgct ttgttgaccg ccttttttac ccgcttcaga
    16261 tgcgcgttgc ggatcatttt taaaattacc gccgctatgc tgaccgcctt tacggccagc
    16321 gtcggatgcc ttttcacggt cttcggcgaa atttcctgaa ccaccacgat gttcggccat
    16381 gttatttctc ccgttgcgtt gcattgtttc attaatatga gtgttgtgtg tcgacactca
    16441 ttaaaattag tcgctaatga aaattagtca aattaagcgc aacgagaaga aagaggg
//
LOCUS       DACBVI010000098        16422 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.098, whole genome shotgun sequence.
ACCESSION   DACBVI010000098 DACBVI010000000
VERSION     DACBVI010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.098"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(34..852)
                     /gene="cof"
                     /locus_tag="IU325_15520"
     CDS             complement(34..852)
                     /gene="cof"
                     /locus_tag="IU325_15520"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HAP5810414.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGCVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            952..2652
                     /locus_tag="IU325_15525"
     CDS             952..2652
                     /locus_tag="IU325_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HAP5810415.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSADGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTV
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPQWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            2717..3412
                     /gene="queC"
                     /locus_tag="IU325_15530"
     CDS             2717..3412
                     /gene="queC"
                     /locus_tag="IU325_15530"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HAP5810416.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(3464..3862)
                     /locus_tag="IU325_15535"
     CDS             complement(3464..3862)
                     /locus_tag="IU325_15535"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HAP5810417.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(3968..4339)
                     /locus_tag="IU325_15540"
     CDS             complement(3968..4339)
                     /locus_tag="IU325_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810418.1"
                     /translation="MKHGIKALIITLSLACAGMSHSALAAAPAAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(4490..6361)
                     /gene="ppiD"
                     /locus_tag="IU325_15545"
     CDS             complement(4490..6361)
                     /gene="ppiD"
                     /locus_tag="IU325_15545"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP5810419.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLE
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDVAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEEQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(6553..6825)
                     /gene="hupB"
                     /locus_tag="IU325_15550"
     CDS             complement(6553..6825)
                     /gene="hupB"
                     /locus_tag="IU325_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HAP5810420.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(7034..9388)
                     /locus_tag="IU325_15555"
     CDS             complement(7034..9388)
                     /locus_tag="IU325_15555"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HAP5810421.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(9576..10850)
                     /gene="clpX"
                     /locus_tag="IU325_15560"
     CDS             complement(9576..10850)
                     /gene="clpX"
                     /locus_tag="IU325_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HAP5810422.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(10976..11599)
                     /gene="clpP"
                     /locus_tag="IU325_15565"
     CDS             complement(10976..11599)
                     /gene="clpP"
                     /locus_tag="IU325_15565"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP5810423.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(11846..13144)
                     /locus_tag="IU325_15570"
     CDS             complement(11846..13144)
                     /locus_tag="IU325_15570"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP5810424.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASIRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(13488..13805)
                     /gene="bolA"
                     /locus_tag="IU325_15575"
     CDS             complement(13488..13805)
                     /gene="bolA"
                     /locus_tag="IU325_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HAP5810425.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            14110..14688
                     /locus_tag="IU325_15580"
     CDS             14110..14688
                     /locus_tag="IU325_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP5810426.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTMTLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNIEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            14732..16207
                     /gene="ampG"
                     /locus_tag="IU325_15585"
     CDS             14732..16207
                     /gene="ampG"
                     /locus_tag="IU325_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HAP5810427.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         3746 a         4415 c         3940 g         4321 t
ORIGIN      
        1 ccgggcaatt gcccggtgct ggaagggatc tcgttattcg ggggaataag gtagatgtgg
       61 atagtccagc cagtgcgtca aatagtgaga gacagcctga tttcggcaat gtccaatcac
      121 cggtaaatgc gggagctccg cgcgcaattg cggcatcgca ttgcccataa taaatccgct
      181 accgacgcag cctaacattt cgcgatcgtt catcgcatca ccaaaggcca tgcaatcgcg
      241 caacgataaa cctaaatgtt gggtcagcac cgtcaatgca gcgcctttat tgcagcccac
      301 cggcagcact tcgaggcaat ccgtagcgga aaaacacaaa tgtgcacgct cgcctaatgc
      361 ttcgtatagc tggatctgca agcgtgtaag atcgtcgtga tcgccacaga agcagatctt
      421 ggtgacgctg ccgagtggca tttttttgac atcgattatc tgataacgaa aaccgctata
      481 gacaaatgcc tgcaacaacg cagggatctc tttcccggta aaccaaccgt cgtcattgaa
      541 gatatgcatg ctggctcggg tatcccattg ctgatacagc accagctccg cgacatccgc
      601 aggtaaatca tcacgatgta aaagttcacc ttccagagaa tgcacgcgcg ttccgttgcc
      661 ggtaatcaaa tatgcatcca gcgatagcgc cccgagaata tgctgcatct ccagcgcatg
      721 acgccccgtg gcaaaagtga gggtaatgtc gcgttcacgc agtcgcgcca aagtagagag
      781 ggttttctca cctaaatgat ggtcaggcat caataaagtg ccatccatat caaatgctgc
      841 cagacgagcc atttctttct ccactctgtg acacggttaa tggtgattga gtatcacctg
      901 atatatacgg aagtaatagt gaatagttaa ataatattgt tccgggttta tatgcgactg
      961 ctcaaccgtc ttaaccagta tcaacgtctg tggcaacctt ccgccggaaa gccgcaaacc
     1021 gtcaccgtca gcgaactggc cgaacgctgt ttttgcagcg aacgccatgt tcgtacgctg
     1081 ttacgccagg cacaggaggc gggatggctg gagtggcagg cgcagtcagg acgcggaaag
     1141 cgcgggcaac tacgctttct ggtcacgcca gaatcgctac gcaatgcgat gatggaacag
     1201 gcgctggaaa ccggaaagca gcaagatgtg ctggagctgg cgcaactggc cccaggtgag
     1261 ctgcgcactc tgttacagcc gtttatgggc ggacaatggc aaaacgatac gcccacgttg
     1321 cgtattccct actatcgccc gctcgaaccg ctacaaccgg gctttttgcc cggccgtgcc
     1381 gagcagcatc tcgccggaca gatattttcc ggcctgaccc gcttcgataa taatacccag
     1441 cgcccgattg gcgatttagc gcatcactgg gaaacctctg ctgacgggtt acgctgggac
     1501 ttttatcttc gttcaaccct acactggcat aacggcgatg cagtaaaagc ctcacactta
     1561 caccagcgat tattgatgct gttacaactg ccagcactgg atcaattatt tattagcgtg
     1621 aagcgtattg aagtcaccca tccgcagtgt ctgaccttct ttttacatcg ccccgattac
     1681 tggcttgcgc accggctggc gagctattgc agccatctgg cgcatccgca attccccctg
     1741 atcggcactg gtccttttcg cttaacacaa ttcacagtgg aactggtgcg cctggagagt
     1801 catgattatt accatttacg tcatccgctg cttaaagcgg ttgagtactg gataactccg
     1861 ccgcttttcg aaaaagattt gggaaccagt tgtcggcatc ccgtgcaaat cactatcggc
     1921 aaaccggagg agttgcaacg ggtcagccag gtcagtagtg gcatcagttt aggtttttgc
     1981 tatttaacgt tgcgcaaaag tccccgactg tccctctggc aggcgcgaaa agtgatctcc
     2041 attattcatc aatccggttt attacaaacg ttagaagtcg gagaaaacct gatcaccgcc
     2101 agtcatgcat tactgccagg ctggactatt ccgcaatggc aagtaccgga tgaggtcaaa
     2161 ctaccgaaaa ccttgacact ggtttatcac ctaccgatag aacttcatac catggcagaa
     2221 cgtctacagg cgacactggc agcggaaggc tgtgaactca caattatttt tcataacgca
     2281 aaaaactggg acgacacgac cctactggca cacgcagacc tcatgatggg cgacagatta
     2341 attggcgaag caccggaata taccctggag caatggctgc gttgcgatcc actatggcca
     2401 catgttttcg acgctccagc atatgcacat ttgcaatcga cactggacgc ggtgcaagta
     2461 atgcctgatg aggaaaaccg atttaatgcc ctaaaagcgg tttttagcca gttaatggca
     2521 gacgcgacgc tgacgccgct attcaactat cactatcgca ttagtgcccc tcccggcgtg
     2581 aacggtgtgc gactgacacc gcgcggctgg tttgaattta ccgaagcctg gcttcccgcg
     2641 ccgtcgcaat gaatagctgg tccgggttaa tcgcaggcgt taccataacg cttttattgt
     2701 tcaagcagga ttatctatga aacgtgctgt cgttgtgttc agtggaggcc aggattccac
     2761 cacctgtctg gtgcaggcat tacaacaata tgatgaagtc cattgcgtga cgttcgatta
     2821 cggtcagcgg catcgcgcag aaatcgacgt ggcacgcgaa ctggcgctga aactgggggc
     2881 acgcgcgcat aaggtgctgg atgtcaccct gctcaacgag ctggcggtca gtagcctgac
     2941 gcgtgacagc attccggtgc ctgattatga acctgaagct gatggcatcc cgaatacgtt
     3001 tgtccctggg cgtaatattt tgttcctgac gctggcggca atatatgcgt atcaggtaaa
     3061 agcagaagcg gtgattaccg gcgtctgtga aacggatttc tccggctacc cggattgccg
     3121 cgatgagttt gtgaaagcac taaaccatgc cgtcagtttg ggcatggcga aagatattcg
     3181 ttttgaaacg ccgctgatgt ggattgataa agcggaaacc tgggcgctgg cagattatta
     3241 cggcaaactg gatttagtcc gtaacgaaac gttgacctgc tataacggca ttaaaggcga
     3301 cggttgcggt cattgtgcgg catgtaattt acgcgccaac ggtttgaatc attatctggc
     3361 cgataaaccg acggtgatgg cagcgatgaa gcagaaaacc gggttgaggt aattattccg
     3421 ggtgtcgccg gatgcggctt gagcatccgg caccacaaaa cgtttactta accatctgct
     3481 cgagcttttc gcgcaattcc ccttccagag ctaacgcttt ctgcgtttta agatcaatac
     3541 aaacaaacgt aataagcgca tccgctacca cctgcccttc cggctccagt gtaatgacct
     3601 ggcttaagat gccgctttta ccgtttaatt gctgcaactg actggtaatg gttaacaggt
     3661 cgcttaatac cgccgggcga cgatagttaa tattgatatt gaccacgacg aaggcgatgt
     3721 tatgggccgt catccactga aaactgtcgc tattttccaa cccatcccag cgggcttctt
     3781 cgagaaactc cagatagcga gcgttgttta cgtgctggta aacgtcgaga tgatatccac
     3841 gaactttgat ttgtgtttgc atagcgcaat aaccttacgt tgtttttata aaaactgtta
     3901 taagtacaga ggccataact ggtatgacct cttcagtctg gcaaaaaatt gccactatgc
     3961 aaattaatta cagggttaat accgccagat tacgttccac cagcgaattg cccatccctg
     4021 gcacctgctt gagatcctcc accgttttaa acggaccgta ctcttcgcga taactgacaa
     4081 tcgcctgcgc tttcttcagg ccaacgccat tcatcgcgcg ggctagctct tccgcgctgg
     4141 cattattaat gctgacccga gtgccttctt cgtcactggc tttcgccggt actgctgctt
     4201 tactttgtgc tgcaggagct tccgctttgg tttctaccgt tgtcggtttc gccgcaggag
     4261 ctgccgccag cgcgctatga gacattccgg cacaggccag agacagggta atgatcagtg
     4321 ctttaattcc gtgtttcata ctgttttctc cttgtttgtt aacagtggaa tcaccttaac
     4381 ggcagtgaaa acagtaaaca aatggcaatg ttcagaaatg gaaaaggccg cgaaagcgac
     4441 cttttcttgt tacagctgca aagcattttt ttgcgaggcg gcttcaggat tattgctgtt
     4501 ccagcgcatc gccaattttg attttcgcct ctttacgcag gttactcatc agagcttcaa
     4561 agacgatttg tgcgttgttc tgggtgatac cctgcaccat cgcttttttc tgttcttccg
     4621 gcattgaacc ttgtttcact tcatccagcg ccagcagaac cacattacct tgcatatcgg
     4681 tcgccatacc gtagctcggt ttgtctttcg ctggcagtgg cagtgcaaac gccgcctggc
     4741 taatcgggtc acgaccggaa cggcttaagg ttttcggctc gccaaatttc agaccggcag
     4801 cctgcatagc ttccgcacct ttgccggctt tcaaatcaac cagcagtttc tcagcatcca
     4861 ctttcgcctg ttgttcagct ttgttatgct gaaccagtgc cttaacttgt tcctgaacat
     4921 ctgccaacgg tttcaccgct tccggtttgt gctcgctgat gcgcagcacg aatgcgcggt
     4981 cgccatcgac ggtgatgatg tcagagttga tgcccggcgc gccgttttca cctaccagac
     5041 cgccgttgaa gatagcgtca gcaaccggct tgaagttcaa ctcttccggc aggttatctt
     5101 tgctgaacca acccgtctga gtggctttaa cgccggctgc ttgctctgca ccggccagag
     5161 actcggtgtc gttgcttgcc gcatcgctca ctttctgttg cagtgcgtag tacgcatcga
     5221 gggctttttc gtgtttcact ttcgccgcga catcgtcacg cacttcgtct aacgacttca
     5281 ctttcgctgg ctgaatgtcg tccagacgta caatcaggaa accgaccgaa gatttgatga
     5341 caccagacag ttggcctttt tctttcaggc cagcattttt tagttcgtcc gggatagtgg
     5401 catcttctaa ccaacccata tcgccgccgt tacgggcaga gataatatcg gcagattttt
     5461 ctttggctaa cgcagcaaaa tcaccgcctt tattcagctc atcaagtacc gctttcgctt
     5521 catcttcagt tttggtctgg atgatgctgt agcgggtacg ctgcggctgg gtgaattgat
     5581 cctgatgctg atcgtagtag ctctggatat ccgcatcgct aaccggttgc tgcatcgttg
     5641 cggcatccag cttgatgtaa ctcacgcgga attgttccgg cgtcatgaaa ttgtttttgt
     5701 tttgttcgta gtagctggca atttcctgtt cggtcacagg ctgcttcgcc gccagcgcgt
     5761 taacatcgat agtcgcttca cgcaccacgc gttgttgagc taccagtgcc gccagctcgt
     5821 cggtttcacc tttcagcata aaatcggtac cggcaacgcc gttaatcagc tgttgggtag
     5881 tgagctggtt acgcagcgcc tgggcgtact gatcggcggt catccccatc tggttgagga
     5941 taccgttata gcggctgtta tcaaatttgc cgtcaacctg gaaggctggg gtcgcgaaaa
     6001 tcgcctgttt aacctgctca tcgctgatac ccagtttcag ctcacgagcg tactgctcca
     6061 gcagcgcctc gtcgatcaga cgattcagca cctgttgacg cagggttttc atatagcctt
     6121 cgttcgctgc aagctcagag tattgatcgc ccagctgttg ctgcatgcga ttacgctcgc
     6181 tgttgaaggc gttctcgaat tgcccacggc tgatttcctg gtcattcact tttgcggcgt
     6241 agttattgcc tccgccaatc aggtaaccac tcacgccggt caatatgaac gacacgataa
     6301 tgataccgaa aataatcttg agcacgagac tgtttgcagc cgtgcgtaag ctgtccatca
     6361 tggtgtaaca acactccgct gtagatgact gtgtacctca cgcaacgtat cgatgcgcgc
     6421 aaaggcctat tgtgacaaga aacgggggca attgtcagcc cacaagacgc agaaagtcac
     6481 cgaatacaaa taaaaaaggc acatcagtag atgcgccctt gaacttcgtc acatccccac
     6541 tggggacaac gcttagttta ccgcgtcttt cagtgcttta cctgcacgga agctcggtac
     6601 tttagcggca gcgatggtga tctctttacc ggtctgcggg ttgcggccag tacgggcagc
     6661 acgctcttta acggcaaaag taccaaaacc taccagtgct acatcatccc cttctttcag
     6721 agattcagtt acggaagcaa taatagcatc taacgcacgg ccagccgcag ctttagagat
     6781 atcagcccct gcagcaatct tgtcgatcaa ttgagattta ttcactcttc tcttcctctt
     6841 tataatttat atcgcacttg aatccttcaa ggtacgaacg cgcagcagtt atatcaggcc
     6901 agccatcccc ttacaacacc ccttaataag ggcaagcccg atccgccatc taacttagcg
     6961 agacaaaaaa aggctggcaa gcccgaatta gcctgccagc cctgttttta ttagtgcatt
     7021 ttgcgcgagg tcactatttt gcagtcacaa cctgcatgcc agacggttca ttttgcagcg
     7081 ccagagtcag aacttcctca atgcgtttca caggatgaat gtccagatcg gcaattacgt
     7141 tgtcaggaat ctcttccaga tcgcgtttat tttcgaacgg aattaacact gttttaatcc
     7201 cgccgcgatg cgctgccagt agtttttctt tcaaaccacc gataggcagt acctgaccac
     7261 gcagagtgat ctcaccggtc attgccacat cggcacgaac cgggttaccg gtcaggcaag
     7321 aaaccagcgc ggtgcacata gcaataccgg cactcggacc atctttcggc gtcgcacctt
     7381 ccggtacgtg gacgtggatg tcgcgttttt cgtaaaaatc agggttgatc cccagttttt
     7441 ccgcacgcgc acgaaccacc gttaacgccg cctgaatgga ctcctgcatc acttcaccga
     7501 gcgaaccggt ataggtcagt ttgcctttcc ccggaacaca tgcggtttca atggtcagca
     7561 agtcaccgcc cacttccgtc cacgccagac cggttacctg accgacacgg ttttcgttat
     7621 cagcgcgtcc atagtcgaaa cgctgaacac cgaggtagtc atgcaggtta tcaccgttaa
     7681 tttcgatatg ttttaatgac ttatcgagca gtaactgctt aaccgctttg cgacacagtt
     7741 tggagatttc acgctccaga ccacgcacgc ccgcctcacg ggtgtaataa cgaataatgc
     7801 cgataatggc gctatcgtcg acggtcagct cacctttttt cagtgcatta cgttcaatct
     7861 gcttcggcag caggtgacgt ttggcgatgt tcagtttttc atcttcggta taaccggaga
     7921 ggcgaatcac ttccatacga tccagcagcg gtgccggaat gttcatggag ttcgacgtcg
     7981 cgacaaacat cacgtcgctg agatcgtaat ccacttccag gtagtggtcg ctgaacgcta
     8041 cgttctgctc tggatccagc acttcaagca gtgcggaggc cggatcgcca cgcatgtcag
     8101 aagacatttt gtcgatctca tcaagcagga acagcgggtt tttcacgccc actttcgcca
     8161 ttttctggat cagtttaccc ggcatagaac cgatgtaagt acggcggtga ccacggattt
     8221 ccgcttcatc acgcacgccg cccagcgcca tacggacata tttacgcccg gttgctttgg
     8281 cgatggactg acccagggag gttttaccta cccccggcgg ccctaccagg cagaggatcg
     8341 gtcccttgat tttgttgaca cggctttgaa ccgcaagata ctcaaggatg cgatctttca
     8401 cgcgctccag accataatgg tcggtatcaa ggatttcctg cgcctgacgc aggtcttttt
     8461 tgaccttgct acgcgcattc cacggcacct gtaccatcca gtcgatataa ccacgcacta
     8521 cggtcgcttc tgccgacatc ggagacatca ttttcagctt ctgcaactct gcttccgctt
     8581 tctcttttgc ctctttcggc attttcgccg cgtcgatttt gcgcttcagg gcttcgtttt
     8641 cgtccggcgc gtcgtccatt tcaccgagtt ctttctgaat agctttcatt tgctcgttca
     8701 gatagtactc acgctgggat ttctccatct gctttttaac gcggttgcga atgcgtttct
     8761 caacctgcag cagatcgatt tccgattcca tcattgccat cagatattcc agacgttcgt
     8821 taacgtcgga catctccaga acggactgtt tgtcagccag tttcagcggc atatgtgcag
     8881 caatggtatc cgccagacgc gccgggtcgt cgatgctatt cagcgacgtc agcacttctg
     8941 gtgggatttt tttgttcagc ttgatgtagc cttcgaactg gctgattgca gtacgcacca
     9001 gcacttcctg ttcccgctca tcaatggtcg gcgactccag atactccgcc ttcgcagaaa
     9061 agtgttcgcc attgtcagag agcgcagaaa tacgcgcgcg ctgtaacccc tcgaccagca
     9121 ctttgacggt gccgtcaggc agtttcagca tctgcaatat agaggccacg gtcccgacgg
     9181 tgaaaagatc gtttacaccc ggctcatccg ttgaagcttc tttctgcgcg accagcataa
     9241 tttttttatc atggtccatc gccgcttcca gacaacggat agatttttcc cgcccgacaa
     9301 ataaggggat gaccatgtgc ggataaacca ccacatcgcg cagcggcaat acggggattt
     9361 caatgcgttc agaacgctca ggattcatag agctctctct tagtttaatt tccgccaggt
     9421 aatcagatga cacgactgtg cttcacgcca tctattaaca tgtacgtcag tatatgggga
     9481 tgtttccccc acattcaacg ccgagaatag aggaaaaatt aaaggggaga taaaatcccc
     9541 cctttttggt taactaattg tatgggaatg gttaattatt caccagatgc ctgttgcgct
     9601 tccggcttgc cataaatcag caacggcttg ctttgaccat caattaccga ttcgtcgata
     9661 accacttttt cgacgtcttc catggacggc agatcgtaca tggtatcgag cagtgcggct
     9721 tctacgatgg aacgcaggcc acgggcaccg gttttacgcg ccatcgcttt cttagcgata
     9781 gcatccagcg cctcgtcacg gaattccaga tccacgcctt ccagattaaa cagcgcctga
     9841 tactgcttgg tcagggcgtt tttcggctct ttgaggatct gaatcagagc ttcttcgctc
     9901 agttcattca acgttgcgac aaccggcaga cgaccaataa actcagggat aagaccaaac
     9961 ttgatcagat cttccggttc aacctgcgcc agcagctcgc cttcgcttgc tttgtcggac
    10021 ttcgctttta ccgtcgcgcc aaaaccaatg ccggagccgg tttctacacg atgggaaatc
    10081 actttatcca gaccggcaaa cgcaccgcca caaataaaca ggatcttaga ggtatcaacc
    10141 tgcaagaatt cctgctgcgg atgtttacgc ccaccttgcg gcggaacagc agctaccgta
    10201 ccttcgatca gtttcaacag tgcctgctgt acgccttcac cggaaacgtc tcgggtaatg
    10261 gacgggttgt ctgacttacg agaaatcttg tcgatttcat cgatgtagac aatcccgcgc
    10321 tgcgctttct gtacgtcgta atcgcacttc tgcaacagct tctgaatgat gttttcaacg
    10381 tcttcgccca cataaccggc ttcggtcagc gtggttgcgt cggccatggt gaacgggacg
    10441 tccagcaggc gcgccagcgt ttcggccagc agcgttttac cggaaccggt cggaccgatc
    10501 agcagaatgt tacttttgcc caactcgaca ccattgctgg tatcgccgtt gcgcagacgt
    10561 ttgtagtggt tgtataccgc gaccgccagc acttttttcg cctgttcctg gccgataacg
    10621 taatcgtcca ggtggttacg aatttcatgc ggcgtcggta gcgcactgcg ttcacgatgc
    10681 ggtgcaactt ctttaatctc ttcgcgaatg atgtcgttac ataaatcaac acattcgtcg
    10741 cagatataca cggatggacc ggcaatcagc ttgcgcactt catgctggct tttgccgcaa
    10801 aaagagcaat acagcaattt gcctgagcca tctttgcgtt tatctgtcat gggtcaaaac
    10861 ctcttctttg ttctttgtgc cgcacacgac gacgcaaatg ccattctcag gcgcaagccg
    10921 cttacagcgt tgtgccgccc tggataagta tagcggcaca gttgcgcctc tggcatcaat
    10981 tacgatgggt cagaatcgaa tcgaccagac cgtattccac cgcttcaggg gcggaaagga
    11041 agcgatcgcg ctcggtatca cgttcaatct gttctaatga ttgacccgta tgaagcgcca
    11101 taagttcatt catgcgtcct ttaactttca gaatttcacg ggcatgaatt tcgatatcgg
    11161 tcgcctggcc ctggtagccg cccaacggct ggtgaatcat cacgcgcgaa ttcggcaggc
    11221 agaaacgttt accttttgcc cctgcagtca gcaagaaagc gcccatcgaa gccgcctggc
    11281 ccatacagat ggtgctgaca tcaggcttga taaactgcat ggtgtcatag atagacatcc
    11341 cggcagtaat caccccgcct ggggagttga tgtacagata gatatctttt tctgggtttt
    11401 ctgcttccag gaacagcatc tgcgccacaa tcaggttagc catgtggtct tcaacctggc
    11461 cagtcagaaa aatgacgcgt tccttaagta gacgagaata gatatcaaaa gagcgctcac
    11521 cacgtgaggt ctgttcaatg accatcggca ccagcgccat atggggtgca aagttatctc
    11581 gttcgccgct gtatgacatt tccgtctcct ggataaaatt gaaaaaacct gctgtaccga
    11641 ttgtaacctt atggacggat tatcagctag tccctgtgtt atgggtacgg catcaccata
    11701 tttcaagcat aacaatcttt tgttgttacg ctaacaccga aagaggcttt tcgcacggtt
    11761 ccatgcaaaa ttcatgacaa aaaaaaagcc caccacctgg cggtgacggg cctttgtgcg
    11821 aatttagcgc gttatgctgc gtaaattacg cctgctggtt catcagctcg ttgaaagtgg
    11881 tttctttttc agtcactttc gctttcgcca gtacagcttc aacagcctgt tcttccagag
    11941 caacattgcg catgttgtcc atcagttctt tgtttttgct gtagaactcg ataacttctt
    12001 tcggatcttc gtacgcagaa gccatctctt cgatcaggcc tttcacgcgc tcttcgtcag
    12061 ctttcagctc gttggtgcgg ataacttcgc ccagcagcag gccaacaact acgcgacgtt
    12121 tagcctgttc ttcgaacagt tcgcgcggca gttccagagc ttgtttttcg ttgccaccga
    12181 aacgctgtgc agcctgacga cgcagaacgt cgatttcact gtcgatcagc gcagccggta
    12241 cgtcgatgtc gttagctttt accagacctt cgatcgcctg agacttaacg cggttacgga
    12301 tggcgctctt cagctcgcgc tccatgtttt tacgcacttc agcgcgcaga ccttctacgg
    12361 aaccatcttc aacgccgaaa cgtttgatga attctgcagt cagttccggc agttcacgct
    12421 cttcaacttt cttcaggttg atagcgaatt tcgctgcttt acctttcagg ttttctgcgt
    12481 ggtattcttc cgggaaggtc acgtcgatgg tgaactcttc gccagctttg tggcctttga
    12541 taccgtcttc aaagcccggg atcatacgac cctggcccat cgccagtacg aaatcagacg
    12601 ctttaccgcc ttcgaactct tcgccgtcta cagaaccggt gaagtcgatg gttacgcggt
    12661 cttctgcttc aacagcgccg tctttttctt tccaggtcgc ctgctgttta cgcagagtat
    12721 ccagcatgcc gtcaacgtca gcgtcggtca cttcaacgat cggtttttca acttcgatcg
    12781 cttccagacc ctgcagttca acttccggat aaacttcaaa ctctacagag taggtgaagt
    12841 cttcacccag cttgtattcg cccggaacat aagtcggtgc gccagccgga ttgatttttt
    12901 ctttaatgat ggcgtcaatg aagttacggc tcatcaggtc acccagaacg tcctggcgta
    12961 tagacgcgcc ataacgctga gcaacgatat tcattggcac tttgcctttg cggaagccgt
    13021 caatacgtac ttttttcgca acgttgacca gctcgctttt aacagcggtc tcgatgctgt
    13081 cagcagcgat agtaatcgtt acacggcggc caaggccttg agtggtttca actgaaactt
    13141 gcatcttgtt acctcaaaaa atcacagtgc tcggtcaact cttaccccgc aagcacaggt
    13201 tgttggcttc gcggaaccgg gatgttctta taatcaatca cattccctgt taccagaatc
    13261 atcccgaaag cattcaaata ggacgcggca ttatagcggc atcactttta tgagtcgaga
    13321 acggttattg cgcgctgtcg cgtatttttt cacaattccc gggcaatttt cgtctgaatg
    13381 ctgggcaaaa agcgcaaaat ccagacaaaa caaaacggcc cgcaggccgt ttttcataaa
    13441 atctttagca acatactgga aaagcgccga cagttgcaaa tgcgttttta cgcgatgctt
    13501 cctgctccac gacagggagg agaggcaaag acggtgtcct gcaacccttc ccactcctta
    13561 atagtgtaag tatgcagagc cagcgcatga acggtagtag agagttcctc cgctaaagta
    13621 ctgtaaatca ttcgatgacg attcagaaaa cgttcacccg taaaacgatc gctgaccagc
    13681 acaactttaa aatggctttc agagccggct gggacattgt gacgatagct ttcatccact
    13741 acttcgagga atacgggttg gaacgccgcc cttaattttt cttctatccg ctcacgtatc
    13801 atcatgaaat atctcctccg acaacgctga gatgtcatcc atccctttaa atactagccg
    13861 cttttaccgt ttccattgca gcttaacaac aaatatttag gtttcgtcct ggtttttctg
    13921 tcaagcgtat gaagtttact gatttaaaga ttgaaacaca ggcctcgcat cagcgtttct
    13981 tttaccaggg gcgattattt cgtcaacatg atgaatttac atgccttacc cacttcccct
    14041 cgccgttggc aatgttatga tggcgggaat ttttaccaaa cacgcttaag agtctctgag
    14101 agcccgaaca tgttcaaaaa aatcctcttc ccgttagttg ctctgtttat gcttgcagga
    14161 tgcgcaaaac cgccaacaac tattgaagtt tccccgacga tgacactgcc acagcaggat
    14221 ccaagcctga tgggcgtcac cgtaagcatt aatggtgccg atcagcgtac cgatcaggcg
    14281 ctggcaaaag tcacccgcga taatcaaatc gttaccctga ccgcctcccg cgatctgcgt
    14341 ttcctgctgc aagaagtgct ggaaaaacag atgaccgcgc gtggttacat ggtcggtccg
    14401 aatggcccgg ttaatctgca aatcatcgtt agccaactgt atgctgatgt gtcccagggc
    14461 aacgtgcgct ataacatcgc gaccaaagcg gatatcgcca tcatcgctac cgcgcagaat
    14521 ggcaacaaaa tgaccaagaa ctatcgtgcc agctacaaca ttgaaggtgc gttccaggcc
    14581 tccaacaaaa atatcgccga tgcggttaac agtgtgctga ccgataccat cgctgatatg
    14641 tctcaggaca ccagcatcca cgaattcatc aagcagaacg cgcgttaatt tctgccctct
    14701 ggcccggtgc aagccgggcc tgtagacgcc catgtccagt caatatttac gtatttttca
    14761 acagccgcgt tcagccatat tgctgatcct gggttttgct tccgggctac cgctcgccct
    14821 gacatccggc accttacagg cctggatgac ggtcgagaat atcgacctca aaaccattgg
    14881 tttcttctcg ctggtaggcc aggcttacgt ttttaaattc ctctggtcac cgctgatgga
    14941 ccgctacacg cccccatttt ttgggcggcg gcgcggttgg ctgctcgcca cgcaaatcct
    15001 gttattagtc gccattgcgg cgatgggttt tctcgaacct ggcacccaac tccgctggat
    15061 ggcggcactg gcagtggtga tcgctttttg ctctgcctcc caggatattg tcttcgatgc
    15121 gtggaaaacc gatgtgctcc cggcagaaga acgtggcgcg ggcgcggcaa tcagcgtgct
    15181 gggttaccgt ttagggatgc tggtttccgg cggcttagcc ctatggctgg cagataaatg
    15241 gctgggctgg cagggcatgt actggttgat ggcggcgctg ttgatcccct gtattatcgc
    15301 gacgttgctt gcaccagaac caaccgacac cattcctgtg ccaaaaacgc tggaacaagc
    15361 ggttgttgca cctctgcgag atttctttgg tcgcaataat gcctggctta ttttgcttct
    15421 tatcgtgctg tataagctgg gcgacgcatt cgccatgagc ctgacaacca cgtttttgat
    15481 tcgtggcgtc gggtttgatg cgggtgaagt aggcgtggtt aacaaaacgc ttggcttact
    15541 ggcgaccatt gttggcgcat tgtacggtgg gattttgatg cagcgcctgt cactgttccg
    15601 ggcgctgcta attttcggta ttttacaagg tgcgtctaac gccggttact ggctgctgtc
    15661 gattactgat aagcatctct acagcatggg cgcagccgtc tttttcgaaa acctctgtgg
    15721 cgggatgggt acatcagcct ttgtcgcgct gttaatgacg ctgtgtaata agtcattttc
    15781 cgctactcaa tttgccctgc tctcagcgct ttctgctgta gggcgagttt atgtcggccc
    15841 cgtggcgggt tggtttgttg aagcacacgg ctggtcgaca ttctatctgt tctccgtcgc
    15901 cgctgccgta ccagggctta ttttgctgct ggtttgccgc cagacgcttg aatatacacg
    15961 agtaaatgac aactttatct cccgtaccga atatcctgca ggttatgcct ttgccatgtg
    16021 gacactggcg gcgggcgtca gcctgttggc cgtgtggtta ctgctgttga cgatggacgc
    16081 gctggatttg acgcacttct ctttcctgcc tgctctgctg gaagtcgggg ttttagtcgc
    16141 cctttctggc gtcgtgcttg gtggtttact ggattatctg gcgctacgaa aaacgcatct
    16201 gacgtaatct gtaaatatta tttagctgta gtaatcactc gccgtaatta ttacggctaa
    16261 ataaacatca gcatcgctta ttaatatttg ttcttttgtg cggcttagcg tctggaaatt
    16321 attgacgcca tttatgaatt ctatttttct tacacgattc agctaatgag tctttatttt
    16381 ctcactaccc agttgtcacc ttaatgataa ttatttgtta aa
//
LOCUS       DACBVI010000099        16413 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.099, whole genome shotgun sequence.
ACCESSION   DACBVI010000099 DACBVI010000000
VERSION     DACBVI010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.099"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(363..1088)
                     /gene="phoU"
                     /locus_tag="IU325_15590"
     CDS             complement(363..1088)
                     /gene="phoU"
                     /locus_tag="IU325_15590"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP5810428.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDPKE"
     gene            complement(1103..1876)
                     /gene="pstB"
                     /locus_tag="IU325_15595"
     CDS             complement(1103..1876)
                     /gene="pstB"
                     /locus_tag="IU325_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HAP5810429.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(1967..2857)
                     /gene="pstA"
                     /locus_tag="IU325_15600"
     CDS             complement(1967..2857)
                     /gene="pstA"
                     /locus_tag="IU325_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP5810430.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(2857..3816)
                     /gene="pstC"
                     /locus_tag="IU325_15605"
     CDS             complement(2857..3816)
                     /gene="pstC"
                     /locus_tag="IU325_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HAP5810431.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(3903..4943)
                     /gene="pstS"
                     /locus_tag="IU325_15610"
     CDS             complement(3903..4943)
                     /gene="pstS"
                     /locus_tag="IU325_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP5810432.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAITKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVLPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(5257..7086)
                     /gene="glmS"
                     /locus_tag="IU325_15615"
     CDS             complement(5257..7086)
                     /gene="glmS"
                     /locus_tag="IU325_15615"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP5810433.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(7248..8618)
                     /gene="glmU"
                     /locus_tag="IU325_15620"
     CDS             complement(7248..8618)
                     /gene="glmU"
                     /locus_tag="IU325_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP5810434.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQADKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(8971..9390)
                     /gene="atpC"
                     /locus_tag="IU325_15625"
     CDS             complement(8971..9390)
                     /gene="atpC"
                     /locus_tag="IU325_15625"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP5810435.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(9411..10793)
                     /gene="atpD"
                     /locus_tag="IU325_15630"
     CDS             complement(9411..10793)
                     /gene="atpD"
                     /locus_tag="IU325_15630"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP5810436.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(10820..11683)
                     /gene="atpG"
                     /locus_tag="IU325_15635"
     CDS             complement(10820..11683)
                     /gene="atpG"
                     /locus_tag="IU325_15635"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP5810437.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(11734..13275)
                     /gene="atpA"
                     /locus_tag="IU325_15640"
     CDS             complement(11734..13275)
                     /gene="atpA"
                     /locus_tag="IU325_15640"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP5810438.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(13288..13821)
                     /gene="atpH"
                     /locus_tag="IU325_15645"
     CDS             complement(13288..13821)
                     /gene="atpH"
                     /locus_tag="IU325_15645"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP5810439.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(13836..14306)
                     /gene="atpF"
                     /locus_tag="IU325_15650"
     CDS             complement(13836..14306)
                     /gene="atpF"
                     /locus_tag="IU325_15650"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP5810440.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(14368..14607)
                     /gene="atpE"
                     /locus_tag="IU325_15655"
     CDS             complement(14368..14607)
                     /gene="atpE"
                     /locus_tag="IU325_15655"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HAP5810441.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(14654..15469)
                     /gene="atpB"
                     /locus_tag="IU325_15660"
     CDS             complement(14654..15469)
                     /gene="atpB"
                     /locus_tag="IU325_15660"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP5810442.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPHNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(15478..15858)
                     /gene="atpI"
                     /locus_tag="IU325_15665"
     CDS             complement(15478..15858)
                     /gene="atpI"
                     /locus_tag="IU325_15665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HAP5810443.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         3979 a         4411 c         4067 g         3956 t
ORIGIN      
        1 aagaacactt tggtgttgtt ttgatacgat tatgactaaa ccacctatga atactgtaaa
       61 aaatcgctca ttttttatgt tccgatatag ggcttttacc ctggtttttt gttttctttt
      121 ccattttctg tgcgggtaac gatataaatg aaaggaataa caaaaaaaat tttataacgg
      181 ggcactacat taaaagtaaa ataattcaaa tgaaataata tatttgcatt gcactgggga
      241 gagtttcgag gtgagtttgt gtggcaatat ttcagggaga aagtgtatca gcccggtaac
      301 tatgtatgac cgggctgatt gccagattcg gcgtaaaagc cttatctgac ctacagttag
      361 cgttactctt tcgggtcttt tccggccagc agtttatcca gctcatcacc gcctacatga
      421 cggaaatcct gccccttcac gtagtagaag ataaactcac aaatattctg gcagcggtcg
      481 ccgatacgtt cgattgaacg cgcacagaat agcgcggtaa gtacgctcgg aatggtgcgc
      541 gaatcttcca tcatgtaggt catcagttga cgaacaatac cttcgtactc ctgatcgact
      601 tttttatctt cacgataaat acgtaccgct tcgtcaatgt ccatccgcgc gaacgcgtcc
      661 agcacgtcgt gcagcatctg gatggtatga cggcccagcg actccagact taccagcaac
      721 ggctgatgct gctgggagaa tttctccagc gcagtacggc agattttgtc cgccacgtcg
      781 ccaatacgct ccagctcggc aatggtttta ctgatcacca taaccagacg caggtcgctc
      841 gccgtcggct gacgtttggc gataatgcgc acgcaggctt catcgatcgc cacttccatc
      901 atgttgacgt tcttgtcgcc ttcgatgacg cgcttcgcca gatcgctgtc ctggttatgc
      961 atcgcggtga ttgcatcaga aagctgctgc tccaccatgc cgcccatggt catcacctgc
     1021 gtgcggatgc tttccagttc ggcgttgaac tggccggaaa tatgtttatt aagattgaga
     1081 ctgtccataa cgcactcctg aatcaaccgt aacgaccggt gatgtagtct tctgtttgtt
     1141 tcttcgctgg cttggtgaac agatcgtccg tgttgctgaa ctcaatcaat tcgcccaggt
     1201 acataaacgc cgtgtggtcg gaacaacgcg cagcctgctg catgttgtgg gtgacgatca
     1261 ccacggtgta atcctgcttc agttcggtga tcagctcttc aatacgcccg gtagagatgg
     1321 ggtcgagtgc cgaacacggt tcgtcgagca gcagcacttc cgggcgaatg gcgataccac
     1381 gcgcaatgca cagacgctgt tgctgaccac cagagagaga gtaaccgctc tgatgcaatt
     1441 tatctttggt ttcgttccac aatgcggctt tggtcaatgc ccactgcacg cgctcgtcca
     1501 tgtcggcacg ggagagcttc tcaaacagac gcacaccaaa ggcgatgttg tcgtagatgg
     1561 acatcgggaa cggcgtcggt ttctggaaca ccatgcccac tttcgcacgc agcagggcga
     1621 tatcctgaga gttggtcagg atgttgtcgc catcgagcag aatttcacct tccgcacgct
     1681 gttccgggta cagttcaaac attttattga aggtacgcag cagcgtcgat ttaccgcagc
     1741 cggacggccc gataaacgcc gttacctggt ttttagcgat atccaggttg atgtttttca
     1801 gggcatggaa tttgccgtag tagaagttca aattacgaac ctgaatttta ctcggggcag
     1861 tttcaaccat actcattgca atcttttcct catcgtgcct gatgcacttc gctaatcagg
     1921 cttaccgtag gccggatcag gcgttcacgc cgcatccggc aaagtgttaa ccgtgtttat
     1981 tcttcgcaaa aacaacgcgc gccagaatgt tcagcagcag gacgcacagg gtaatgatca
     2041 ataccccggc ccaggccagt tgctgccatt ccgcaaacgg actcatcgca aacttaaaga
     2101 tcgtcaccgg caggttggcg atcggctgca tcatgtccgt gctccagaac tggttggaga
     2161 gcgcggtaaa cagcagcggc gcggtttcac cggcaatacg ggcaatcgcc agcaggatac
     2221 cggtcataat cccggacacc gacgctttca gcgtaatcgc ggagatcatc ttccacttcg
     2281 gcgtacccag cgcataagcc gcttcacgca ggctgtccgg caccagtttc agcatgttct
     2341 cggtggtgcg gataacaatc ggcacctgca acagcgccag cgcaatcacg cccgcccagc
     2401 cggagaagtg ctccatctgc gccaccacga tggtgtaaac aaacagacca actacaatcg
     2461 acggtgcgga gagcagaatg tcgttaataa agcgaatcac ttctgccagc caggatttac
     2521 gaccatattc cgccagataa atccccgcca taatgcccag cggcgtaccg aataccgtgg
     2581 cccacaaaat taacagcccg ctacccgcca gggcgttcgc cagaccaccg ccttccgtat
     2641 tgggcggcgg cgtcatttca gtgaacagcg ccagcgacat accatcgata ccgcgagtga
     2701 tggtggacat taaaatccag atcagccaga acaggccgaa ggccatcgtc gccattgaga
     2761 gcgtcagcgc aatacggttt ttgaggcgac gacgcgcctg cattttgcgg cgagattcag
     2821 ccagcgccgc agtggtttgc atttcaacca tagccattag cgtgccccct cattcttagc
     2881 caggcgcata atcataaact tcgatgcggc gaggacgatg aaggtaatca caaacaggat
     2941 caggcccagt tccatcagtg cggcaacgtg cagaccggat tccgcttcgg caaattcgtt
     3001 cgccagcgca gaggtgatac tgttgcccgg catgtacagc gaggcgctgt cgagctgata
     3061 ggtgttaccg atgataaagg tcaccgccat ggtttcaccg agcgcacgcc ccagccccag
     3121 catgatgccg ccgataaccc catttttggt gaacggaaga acgatacgcc agataacttc
     3181 ccaggtggtg cagccaatac cgtaggccga ctctttcatc atcaccgggg tttgttcgaa
     3241 cacatcgcgc attaccgccg caatgtacgg aataatcatg atggcgagga tcacgcctgc
     3301 cgcaaggata ccgataccaa atgcggggcc agagaacagc gcgccgacaa tcgggatgtt
     3361 cgacatgata ttgccgaccg gctcctggaa gtaaacggcg aacagcggcg caaagataaa
     3421 caggccccac atgccgtaaa cgatacttgg aatggcagcc agcagctcaa tggcgatacc
     3481 cagcgggcgt ttcagccagc caggcgcaag ctcggtcagg aacagggcga taccaaaact
     3541 caccggaacg gcaatcagca gcgcgataaa cgaggtcacc agcgtaccgt agatcggcac
     3601 cagcgccccg tagatatcgt tcggcgcatc ccactctttg gtccacagga aagccagacc
     3661 aaatttctga atgctcggcc aggaggagat gatcagagag acaataatgc cacccaacat
     3721 caatagcaca atcagcgccg ccagttttac tagcacgctg aaaattatgt cgcccttttt
     3781 acccggtggg ttaaaagcag gcttggttgc agccataagt tactcttcag ttaaacgcgt
     3841 ttacaaagtc attttgtaat gccggatgcg gcgtaaaaca ccgtacccgg cctggagttt
     3901 tattagtaca gcggcttgcc gctactgtct ttaatattgg tcttccacgc agcgcgaacc
     3961 tgctcaacta cgctatccgg caggctggca taatccaggt cgttcgcctg tttagccccg
     4021 gttttgtatg cccagtcgaa gaacttcagc acttcggtgc cttgttctgg tttcttctga
     4081 tctttatgga tcagaatgaa cgtggtagag gtaataggcc atgcatcttc gcctttctgg
     4141 ttggtcagat cctgagcgaa tgttttgctc cagtctgcgc cttttgcagc gttagcgaag
     4201 ttttcttcgg tcggcaaaac aggtttacca tcagcggaga tcagtttggt gtacgccagg
     4261 ttgttctgct tcgcgtaagc atattcaacg taaccaattg cacctggcag acgctgaacg
     4321 aacgcggcga taccgtcgtt acctttaccg cccagaccaa tcggccattt tacggtagag
     4381 cccgtaccaa cgttgttttt ccactcttcg ttcactttcg ccaggtagct ggtgaagacg
     4441 aaggacgtcc cggagccatc tgcgcggcgt actacagcaa tgttctgtga cggcagtttt
     4501 aagcctggat tcagtttggt gatggcttca tcatcccact tcttgatttt gccgaggtag
     4561 atgtcaccaa gggttttacc atccagcacc agctcgccag acttcaaccc tggaatgtta
     4621 accgccagta ccacgccgcc aatcacggtc gggaactgga acaaaccttc ctgagccagt
     4681 ttttcgtcag acagcggcgc gtcagaggca ccgaaatcaa cggtatttgc gataatctgt
     4741 tttacgccac cggaagaacc gataccctgg tagttaactt tattaccggt ttctttctgg
     4801 taagtgtcag cccatttggc atacaccggc gcagggaagg ttgcacctgc acctgtcagg
     4861 cttgcttctg caaacacaga gaaagcactc atcgataagg tcgcggcgac aacagttgcg
     4921 acggtggtac gcataacttt cataatgtct cctgggagga ttcataaagc attgtttgtt
     4981 ggctacgaga agcaaaatag gacaaacagg tgacagttat atgtaaggtt tatgacagtt
     5041 ttatgacaga gagagaaagt ctttagtctg atttaaataa gcgttgatat tcagttaatt
     5101 acaaatatta ataacgaaga gatgacagaa aaattttcat tctgtgacag agaaaaagta
     5161 gccgaagatg acggtttgtc acatggagtt ggcaggattt ttgattaaaa acataacagg
     5221 aagaaaaatg ccccgcttac gcagggcatc catttattac tcaaccgtaa ccgatttcgc
     5281 caggttacgc ggctggtcaa cgtcggtgcc tttgatcagc gcaacgtggt aagccagcag
     5341 ctgcagcgga acggtgtaga agatcggtgc aatcacctct tccacatgcg gcatctcgat
     5401 gatgtgcatg ttatcgctac ttacaaaacc cgcatcctga tcggcgaaga catacaactg
     5461 accgccacgc gcgcgaactt cttcaatgtt ggatttcagt ttttccagca attcgttgtt
     5521 cggtgcaacg acgataaccg gcatatcggc atcaatcagc gccagcggac cgtgtttcag
     5581 ttcgcctgca gcgtaggctt cagcgtgaat gtaagagatc tctttcagct tcaatgcgcc
     5641 ttccagcgcg attgggtact gatcgccacg gcccaggaac agcgcgtgat gtttgtcaga
     5701 gaaatcttct gccagtgctt caatgcgttt atcctgagac agcatctgct caatacggct
     5761 cggtagcgcc tgcagaccat gcacgatgtc atgttcaatg gaggcatcca gacctttcag
     5821 acgagacagc ttcgccacca gcatcaacag cacggttaac tgggtggtga atgctttagt
     5881 ggacgccacg ccgatttctg tacccgcgtt ggtcattagc gccagatcgg attcgcgtac
     5941 cagcgaagaa cccggaacgt tacagattgc cagtgaacca aggtaaccca gctctttcga
     6001 cagacgcagg ccagccaggg tatccgcggt ttcgccagac tgtgacaagg tgatcatcag
     6061 gctgttacga cgcacggcag atttgcgata gcggaattca gaggcgattt cgacgtcgca
     6121 cggaatacct gccagcgatt caaaccagta gcgggaaacc ataccggagt tataagaagt
     6181 accacaggcg aggatctgaa tatgctcaac cttcgacagc agttcgtcgg cgttcggtcc
     6241 cagctcgctt aaatcaacct gaccgtggct gatgcgcccg gtaagggtgt ttttgatcgc
     6301 gttcggctgt tcgtagatct ctttctgcat gtagtggcgg taaatacctt tatcgcccgc
     6361 gtcatattgc agattggatt cgatatcctg acgttttact tccgcgccag ttttatcgaa
     6421 gatgtttacc gaacggcgag tgatttccgc aatatcgccc tcttcaagga agataaagcg
     6481 acgagtcact ggcaacagcg ccagctggtc agaagcgata aagttttcgc ccatacccag
     6541 gccaatcacc agcggactac cagaacgtgc cgccagcagg gtatccggat gacgggagtc
     6601 catgatcact gtaccgtacg caccacgcag ttgcgggata gcacgcagaa cagcctcacg
     6661 cagagtaccg ccttgtttca actcccagtt caccagatgg gcaatcactt cggtgtcggt
     6721 ttcagaaacg aaggtatagc cacgcgcttt taactcttca cgcagcggtt catggttttc
     6781 gatgatgccg ttatgcacca ccacaatgtg ttcagaaaca tgcggatgcg cgttagcttc
     6841 tgaaggttca ccgtgggtcg cccagcgagt atgagcaata ccggtgccgc catgcagagg
     6901 atgttcttcc gctgcctgag ccagcatctg gactttaccg aggcgacgca ggcgggtcat
     6961 atgaccttct gcatcaacaa cggccagacc ggcagagtca tatccgcggt attcaagacg
     7021 acgtaaacct tcaagaagga tttctgctac atcacgttgc gcgatcgcgc caacaattcc
     7081 acacatagtt tttgattccg atttatatcg ttgtcggtca acctgtatgc ccgttttttc
     7141 gggcaccccg agccttgtag agagtggggt tatttttata gttactgctt gtgggcggga
     7201 gattatgtta tctcctcatc ccatatgacc gtattacccg gccagaatta ctttttcttt
     7261 accggacgac gccagccttc tttttgagtc tgcggcacac ggctgatcgc cagggcgttt
     7321 tcgccgacat tacgcgtcac agttgtaccc gcagcaatgg tcgcgccttt gcctactgtt
     7381 accggggcca ccagctgagt gtcggaaccg acaaacacat cgtcaccgat aatggtctta
     7441 aatttattcg caccatcgta gttgcaggta atggttcccg cgccgatgtt aacgttatcg
     7501 ccaatttccg catcgcccag gtaagtcaga tgaccagctt tcgagccttt acccagacgc
     7561 gcttttttca tctcaacgaa gttaccgacg tgtgcacctt ccagcaactc agcaccagga
     7621 cgcagacggg caaacgggcc aatggtacag gccgctgcca gattcgcatc ttccacaacg
     7681 gtatacgggc taatttcgca atcatcgcca atcacgctgt ttttaatcac gcaaccggtg
     7741 ccgattttca cgcgatgacc gagagtcacg ttgccctcga tgataacgtt agtatcaatt
     7801 tcaacatcgc gcccgtgagt aagcgtaccg cgcagatcaa aacgcgccgg atcgcgcagc
     7861 ataacgcctg ctaacagcag tttatcagcc tgttcggact ggtaaacgcg ctccagtcgg
     7921 gagagttgca ggcggttatt cacgccttct acttcgctta aacgttgcgg atgaacggcg
     7981 acgatttcac gcccttcctg atacgccagc gcaataatgt cggtgatgta gtattcaccc
     8041 tgagcgttat tgttggtcag cttcgccagc cagcgtttca tatctgcgcc gttggcaatc
     8101 agaatgccgg tgttgatctc ctgaatctga cgctgctcgt cggtggcgtc tttgtgctca
     8161 acaatgccgg taactttgcc gttttcacgg gtgatacgtc cataaccggt cggatcatcc
     8221 agttttaccg tcagcagacc aatgccaccc tgcggtttag catcacgcag gcgctggagt
     8281 gtttcgacag agatcagcgg cacgtcgccg tagagcatta aaatgtcttc atcatcggca
     8341 aagaaaggtg cggcctgctg catcgcatga cctgtaccca gctgttctgc ctgaagcacc
     8401 cagttcaggt tgtcatcttt cagcgcctgt ttaagcagat cgccgccgtg accgtacacc
     8461 aggtgaacgt gcgccgcgcc taattcattc gcagcatcaa tgacatgctg aaccatcgct
     8521 ttcccggcaa gggtatgcag cactttcgga agatcggaat acatgcgcgt gcctttgcct
     8581 gcggcaagga tcactacgct catagcatta ttcaacatac gcgtcctgac tgtaatttga
     8641 gaacgaattt aaaccgcttc accttgaaaa aactacattt ttttcatcgt gaaatggaca
     8701 gaggataaat tgttcaataa cggattatcc ccgctgacga aagtgccatt ttcgaccatc
     8761 gtcacgcctt ttgtctgcag aaaattaagg cagtaccttc atttaagcgt caggtggatg
     8821 tttttgctct tatttcgatc aatgaataaa cgggaaaaca gggtgtttta tatcagcagg
     8881 atctatgtga acgctcttca ggacgggtca atacgcgtaa aaaaaagcca gcctgttacc
     8941 agactggctt ttgtgctttt caagccggtg ttacatcgct tttttggtca actcgataac
     9001 gcgcagctgc gcgatcgctt tggccagttc cgcagacgcc tgagcgtaat ctacgtcgcc
     9061 gtgagagctg ctaatgtgct cttcagcctt acgtttcgct tccatggctc gcgcttcgtc
     9121 gagatcctgg ccgcgaattg cggtgtcggc cagaacggtc acgttgccag gctgcacttc
     9181 aagaatgccg ccagacagat agataaactc ttcgtgaccg tgctgtttca cgatgcgaat
     9241 cataccaggc ttaatggcgg tgagcagcgg tgcgtggcca gggtagatcc ccagttcacc
     9301 ttcgctaccc gttacctgga ttttctcgac cagaccagag aacatttgtt gctctgcact
     9361 gacgacgtcc aggtggtaag tcattgccat atcaccctcc gattaaggcg ttaaagtttt
     9421 ttggcttttt ccacagcttc ttcgatggaa ccgaccatgt agaacgcctg ctccggcagg
     9481 tgatcgtatt cgccttccat gatgccttta aagccacgga tggtgtcttt cagggagacg
     9541 tatttacccg gagaaccggt gaatacttct gccacgaaga acggctggga caggaagcgc
     9601 tggatcttac gagcacgcgc taccaccagt ttgtcttctt cagacagttc atccataccc
     9661 aggatggcga tgatgtcttt cagttcctga taacgttgca ggatggactg aacgccacgc
     9721 gcggtgtcgt agtgttcctg accaaccacc agcgggtcca gctgacggct ggtggagtcc
     9781 agcgggtcaa cggccgggta gatacccaga gacgcgatct gacggctcag taccacggtt
     9841 gcgtcaaggt gcgcaaaggt ggttgccgga gacgggtcag tcaagtcatc cgcaggtacg
     9901 tataccgcct gtacggaggt gatagaacca gttttggtgg aggtgatacg ttcctgcaga
     9961 acgcccatct cttccgccag ggtcggctga taacctaccg ctgaaggcat acggcccagc
    10021 agtgcggata cttccgtacc ggccagggtg taacgataga tgttgtcaac gaacagcaga
    10081 acgtcacgac cttcgtcacg gaatttctca gccatggtca gaccggtcag agcaacgcgc
    10141 agacggtttc ccggcggctc gttcatctgg ccatacacca gggatacttt gtcgataacg
    10201 ttggagtcgg tcatttcgtg gtagaagtcg ttaccctcac gagtacgttc acctacgccc
    10261 gcaaacacag agtaaccgga gtgctcgatc gcgatgttac gaataagctc catcatgttt
    10321 acggttttac ctacgcccgc accaccgaac agaccaactt taccgccctt agcgaacgga
    10381 cacatcaggt cgataacttt gataccggtt tccagcagtt cctgagagtt tgacagctct
    10441 tcgtaggaag gtgctgcgcg gtgaatcgcc caacgctctt cttcaccgat ctcgcctttc
    10501 atgtcgaccg gttcacccag tacgttcatg atacggccca gagtcgcttt acctaccggg
    10561 acttcgatcg ggtgttcgag gtcttttaca tccagaccgc gacgcagacc gtcggaggaa
    10621 cccattgcga tggtacgcac gataccgccg ccgagctgct gctgaacttc cagcaccaga
    10681 cgctcattac cattttgcac ctcaagagca tcgtacacgc gcggtacggc atcctgaggg
    10741 aattcgacgt caactacggc gccgattacc tggacaatct ttccagtagc catcttaaat
    10801 cctctacgaa ataacctgtt taaaccgcgg cggcccccga gacgatctcg gtgagttcct
    10861 gagtaatgct ggcctgacga gctttgttgt ataccaactg cagctcttta atcaggctgc
    10921 cgccattgtc ggtcgcggct ttcatcgcca ccatacgggc ggcctgctcg ctggccaggt
    10981 tttcaaccac gccctgataa acctgagatt cgacataacg acgcagcagg gtatccagca
    11041 acgccttcgg atcgggttcg tacaggtaat cccaggattt gtgtttcaga tcatcatcat
    11101 ctgatgccgg taacggcagc agctggctga tggtcggaac ctgagacatg gtgttaataa
    11161 atttgttgct gacaatgtaa agcttgtcca gacggccttc gtcgtaggcc tgcaacatca
    11221 cttttaccgg accgatcagt tcggacaggg aagggttatc ccccatgcca gtgacctggg
    11281 caacaacatt gccgcccacg gagttgaaga acgacacgcc tttcgagccg atcattgcga
    11341 ggtcgcattg aacgcctttg tcggtccagg tcttcatttc cgccagcagt tttttgaaca
    11401 ggttaatgtt caaaccaccg cacaaaccac ggtcggtcga caccaccagg tagcccacgc
    11461 gtttaacgtc gcggtcttcc aggtaagggt gcttatattc cagattaccg tgtgcaaggt
    11521 gaccaatcac tttgcgcatg gtttctgcat aaggacggct ggccgccatg cgatcctgcg
    11581 atttacgcat tttggaagcg gcgaccatct ccatcgcttt agtgatcttt tgcgtgttct
    11641 ggacgcttgc gatcttacta cgtatctctt ttgcgccggc catgagcttc tcctcaatgc
    11701 cttgcggcct gccctaaggc aagccgccag acgttaccag gattgggttg ctttgaagga
    11761 atcgaggatg cctttcagct tgccttcgat ttcgtcgttg tagccaccgg tctggttgat
    11821 ctcttgcatc aacggagcgt gatcacggtc gacgtaagcc agcagagcgg cttcgaagct
    11881 gccgattttc gacagttcaa catccgccag gtaaccacgt tctgctgcga acagaaccag
    11941 agactgttgc gctacggaca tcggcgcata ctgtttctgt ttcagcagtt cggtcacttt
    12001 ctgaccgtgg tcaagctgct tacgtgttgc atcgtcaagg tcggatgcaa actgagagaa
    12061 cgctgccagt tcacgatact gtgccagagc ggtacggata ccaccggaca gttttttcat
    12121 gatcttggtc tgtgctgcac caccaacacg ggatacggaa atacccgggt taaccgcagg
    12181 acgaataccg gcgttgaaca ggttggtttc caggaagatc tgaccatcgg taatggagat
    12241 tacgttggtt ggaacgaacg cagaaacgtc acccgcctga gtttcgataa tcggcagcgc
    12301 agtcagagaa ccggttttcc ctttcacttc acctttggtg aaggcttcaa cgtattcggc
    12361 gttaacacgc gcagcacgct ccagtagacg agagtggagg tagaatacgt cgcccgggaa
    12421 tgcttcacgt cctggcggac gacggagcag cagggagatc tgacggtaag caacagcctg
    12481 tttagacagg tcatcgtaaa tgatcagcgc atcttcaccg cggtcacgga agtattcgcc
    12541 cattgcgcaa ccagcgtacg gtgccaggta ttgcagtgca gcggattcag acgcggttgc
    12601 taccacaacg atggtgttag ccagtgcgcc gtgctcttcc agtttacgta ccacgttaga
    12661 aatggtggac gctttctggc cgatagcgac atagatacat ttgataccgg aatcgcgctg
    12721 gttgatgatg gcatcgatag ccaatgcggt tttaccggtc tgacggtcac cgatgatcaa
    12781 ttcacgctga ccacgaccga ttgggatcat ggagtcaacg gctttataac cggtctgtac
    12841 cggctgatct acggactgac gttcgataac gcccggagcg attgcttcta cagcagagaa
    12901 gccgtcgtga tccagcggac ctttaccgtc gattggtgca cccagagtgt taaccacacg
    12961 gcccagcagg ccacggccaa ccggaacttc caggatacgg ccagtacact taactttcat
    13021 gccttcggca aggtcagcgt acggacccat aacaaccgca cctacagagt cgcgctcgag
    13081 gttcagtgcg atagcgtaac ggttacccgg cagggagatc atttcaccct gcatacaatc
    13141 ggccaggccg tgaatgcgga taacaccgtc acttacagaa acaatagtac cttcgttgtg
    13201 agcttcactc acaacattga actgagcaat gcgctgcttg atcagttcgc tgatttcggt
    13261 ggaattcagt tgcatgctcc agtcccctta agactgcaag acgtctgcaa ggcgctcaag
    13321 acgaccgcgt acgctgccat caatgaccat atcacccgct cggatgataa cgcctgccat
    13381 tacagactta tcgattttgc aattcagctt aactttgcgt gacagacgtt tttccatcgc
    13441 agcagaaatt ttcgcgagct gttgttcact cagtgcggca gcggaaatga cgtctacctc
    13501 agcggtagcc tcactcacgg cacgcaggtg aataaactgc tccagaacat ccgggagcgc
    13561 gttaagacga ccattttcag ccattacccg aatcaggttc tgaccgtttt cgtccagttg
    13621 ctcaccacaa actgcgataa acgactcggc gagcgtttct ggcgcaagcg cgccagagag
    13681 aagctctgcc atttgttcgt ttttggttac ctcggcggca aacgccagca tgtcctgcca
    13741 gcgttctaca ctttggtgtt cgacggcaaa gtcaaaagct gctttggcgt aggggcgagc
    13801 taccgtaata aattcagaca tcagcccctc cctccttaca gttcagcgac aagtttatcc
    13861 acgatgtcgc tgttagcagc ttcatccacg gaacgttcga tgatcttctc ggcgccagca
    13921 acagccagga tagcaacttg cttacgcagc tcttcacggg cacgtttacg ctcggcttca
    13981 atttccgcct gcgcctgggc cacgatttta gtacgttcct gttctgcctc agctttcgct
    14041 tcgtccagaa tctgcgagcg gcgtttgtta gcctgctcga tgattacctg ggcttccgct
    14101 ttcgcttttt tcagctggtc ggtcgcgctg gcctttgcaa ggtcaaggtc cttatgtgct
    14161 cgttctgcgg aagcaagacc gtcagcaatt tctttttgac gtttttcgat ggctgccatt
    14221 aatggcggcc atacgtactt catgcagaac agaacgaaca ggacaaacgc gatggcctgg
    14281 ccgaggattg ttgcgttaag attcacagca caatgcctct atttagttaa cgttctgata
    14341 ttgctcttta aataaaagca acgcttacta cgcgacagcg aacatcacgt acagacccag
    14401 acctacagcg atcatcggga tagcatccac cagacccata acgataaaga actgagtacg
    14461 cagcagagga atcagatcag gttgacgcgc tgcgccttcc aggaatttac ccccgaggat
    14521 gccgataccg atcgcagcac cgattgccgc cagacccatc atcacagcgg cagccatgta
    14581 cagcagatcc atattcaggt tttccatgac agtctccagt ttgtttcagt taaaacgtag
    14641 tagtgttggt aaattaatgt tcttcagacg ccatcgacag atagacgatc gtcagaacca
    14701 tgaagatgaa ggcttgcagc gtaatgatca ggatgtggaa aatggcccac ggcacattca
    14761 gaatccactg tgaccaccac ggcaacagac cagcaatcag aatgaaaatc agctcaccgg
    14821 catacatgtt accgaacagt cgcaaaccga gtgaaactgg tttggacagc aggcttaccc
    14881 cttcaaggat taagttgaca ggaatgaacg cccagtgatt gaacggctgc agcgtcaact
    14941 ctttcgtgaa gccgccgatg cctttcattt tgatgctgta gaacagaatc aggataaata
    15001 cacccagtgc catagacagc gttacgttca cgtccgcaga cggaaccaca cgcagtgcag
    15061 gcagacccag tacatgttca gcaatgtacg gcagcaggtc gataggcagt aaatccatca
    15121 ggttcatcag gaatacccag acgaagatcg tcagggccag cggagcaatc agcttgcttt
    15181 tgccatggta catgtctttc acgctaccat taacaaagcc gatcaccagc tcaatcgcgg
    15241 tctgaaactt acctggcaca ccgctggtcg cctttttggc tacgctacgg aataaaacca
    15301 ggaacaacag acccagcacc accgagaaga acatggagtc aatattgatt gtccagaagg
    15361 tggctggggg gttatgtgga tccaccagcg agaatgtacg caggtccagc tgaaggttgt
    15421 tcaggtggtg tcctatgtaa tcctgcggcg tcatattttc tgaagccatg atgcctttta
    15481 ccctttgttg ttaattacag ccggtgccag tatctgaacc accagcacca aaacccacgt
    15541 aacgatcagc ggcaagaata ccgcctttaa aaccgccaac gccaccacca gtaacaccaa
    15601 catcgccaga actttgaaag cttcgccaaa tgcgaatgtc caggccaccc ggcctttcgc
    15661 tggtgtatgc gcctggtgac gccaggcaaa tatcataaac aaaacgttag gcagaaagac
    15721 tgccaggccc ccgcttattg cagagacgcc ccagaagggg tctttgaggc tgaacagcaa
    15781 tccacttgct atcaccacca gtaactgaac gagcagaagc ttccgagcaa cgtttcgact
    15841 cacgagcgac acagacatca cgtttttcac tcctgctccc ttcgaggtat gccgcgtgtc
    15901 gtataaaact ttctttaagg cttagagtca agcatcaaaa agcggtcaaa ttatacggtg
    15961 cgcccccgtg atttcaaaca ataagtagcc aaaaggtgaa taaatgttta aatatttttc
    16021 cagtgcatac aattgcgact tttctgcaaa ccctgttcga tcatgaaaaa ctgtaaataa
    16081 cgcgtaaaca ctggtgataa agcgtgcttc agatcacata ttgagcatgt tcgcgcacag
    16141 catatttatt tacttggcaa atgatgcctt tgcaagttta tgatatttca gtctaaaaac
    16201 agatactgtt ttaataaatg acatttacac aacaaaaacc acccattgac atttttaata
    16261 atgttttaac agccaatgat gattcttagc gccgattttt agcagactga tattttcact
    16321 tatgacttat tttctgctta ccaaaaaaag ccacgttact ttgttgatgc aaaagagtga
    16381 acgtggcgtt aaatgtaacc agttatatca gta
//
LOCUS       DACBVI010000100        16295 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.100, whole genome shotgun sequence.
ACCESSION   DACBVI010000100 DACBVI010000000
VERSION     DACBVI010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.100"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            48..698
                     /gene="zinT"
                     /locus_tag="IU325_15670"
     CDS             48..698
                     /gene="zinT"
                     /locus_tag="IU325_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HAP5810444.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            1041..1571
                     /locus_tag="IU325_15675"
     CDS             1041..1571
                     /locus_tag="IU325_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HAP5810445.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(2141..2230)
                     /locus_tag="IU325_15680"
     tRNA            complement(2141..2230)
                     /locus_tag="IU325_15680"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2194..2196),aa:Ser,seq:cga)
     gene            2324..3121
                     /gene="mtfA"
                     /locus_tag="IU325_15685"
     CDS             2324..3121
                     /gene="mtfA"
                     /locus_tag="IU325_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HAP5810446.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHRTNDTDSFSATNVH"
     gene            3222..3297
                     /locus_tag="IU325_15690"
     tRNA            3222..3297
                     /locus_tag="IU325_15690"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3255..3257,aa:Asn,seq:gtt)
     gene            3610..11593
                     /locus_tag="IU325_15695"
                     /pseudo
     CDS             3610..11593
                     /locus_tag="IU325_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin-like protein
                     YeeJ"
     gene            complement(11855..12907)
                     /locus_tag="IU325_15700"
     CDS             complement(11855..12907)
                     /locus_tag="IU325_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase family
                     protein"
                     /protein_id="HAP5810447.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNVLKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMLFISQLKAKTNFQVVDLTMKCYSPVCKNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADISESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            13222..14538
                     /gene="shiA"
                     /locus_tag="IU325_15705"
     CDS             13222..14538
                     /gene="shiA"
                     /locus_tag="IU325_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HAP5810448.1"
                     /translation="MDSTLISTRPDERTVSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAMYLLAGCLISAITALLMKDSQRA"
     gene            14640..16094
                     /gene="amn"
                     /locus_tag="IU325_15710"
     CDS             14640..16094
                     /gene="amn"
                     /locus_tag="IU325_15710"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HAP5810449.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         4483 a         3873 c         3788 g         4151 t
ORIGIN      
        1 caatataaca ttacacatca tatacaatga ctctggagga aactgttttg gcgattcgtc
       61 ttcacaaact ggctgttgct ttaggtgtct ttattgttag cgctcctgcc ttttcgcatg
      121 gtcatcactc acacggcaaa cccttaacag aggtcgaaca aaaagctgct aatggtgttt
      181 ttgatgatgc caatgtacaa aaccgaacgc tcagtgactg ggatggagtc tggcaatccg
      241 tttatccttt actgcaaagt ggcaaacttg accccgtctt tcagaagaaa gcggatgcag
      301 ataaaactaa aacatttgct gaaattaaag attattatca caaaggttat gcaacagata
      361 tcgagatgat tggcattgag gacggcattg ttgaattcca tagaaataat gaaacaacat
      421 cctgtaaata tgattacgat ggatacaaaa tactcaccta taaatcaggc aagaaaggcg
      481 ttcgttactt attcgaatgt aaagatcctg aaagcaaagc ccctaaatat atacaattta
      541 gcgatcatat aattgcacca cgaaaatcat ctcattttca catttttatg ggtaatgatt
      601 cacagcaatc tttattaaat gaaatggaaa actggccaac gtattatcca tatcagttga
      661 gtagcgaaga agtggtcgag gaaatgatgt ctcattgagt attctcatga taacgcctcg
      721 atgccgcttt agtaagtcat cataactgcc actggtccat ccacaaacgc cactgaacgc
      781 aagctagcta cagacacgct catcactatg acgtgtctgt atattaataa gctaacccgc
      841 attgagttaa ccaataacgg attccataca caatacggcc aacagagaaa gatacctgtg
      901 ctcacgccat tgcttatatt ggctggttac aatgtgcact atcaattttt taaataacaa
      961 attcttaaaa ccacatctgt ataaggattt tttaaggttc gtgggatagc ttgactgtaa
     1021 aaattacagg agctacaaaa atgaaccgat tctcaaaaac tcaaatttat ttacattgga
     1081 taacgctgct tttcgttgca ataacctatg ccgcgatgga actccgtggc tggtttccta
     1141 aaggtagtag tacttatctg ctgatgcgag aaacacatta caatgcgggt atattcgttt
     1201 gggtgttaat gttttcacgc ctgattataa aacaccgtta tagtgatcct tctattgtgc
     1261 caccgccacc tgcctggcaa atgaaagcgg cttcgctaat gcacatcatg ctttatataa
     1321 ccttccttgc acttcctctg ctggggattg ctttgatggc ttacagtgga aaatcgtgga
     1381 gtttccttgg tttcaatgtg tctccctttg ttaccccaaa cagcgaaatt aaagcactga
     1441 taaaaaatat tcacgaaacc tgggcaaata taggttactt tttaatcgca gctcacgctg
     1501 gcgcagcact ctttcatcac tacattcaga aagataatac tctgttacga atgatgcctc
     1561 gccgcaaata agtcgatccc ctgacaagga aatgtgaacc atggacatca acgcgtcata
     1621 aatgtttacg caagtgcaca atgcaccctt tccactatca acaaggagga gcagaaactg
     1681 tgtaaatcgt acaatggtaa gacttatcaa aaatacaggt taattttggt atttaaacct
     1741 tttttacttg ttagtatgat acaaaggctt tcaaaaaagc tgggaaattc aacaactctt
     1801 ggaaattatg gactttgtcc cgcggcttta atctgacggc cgcgttcctt ttttgactca
     1861 attattgacc ccttctctac gcaacttcag ttcccaccac caactttgcg gcagtttcat
     1921 aggatgaatg tctaaaagaa taatggtgag cgataagaaa acgactgaat aactgcacat
     1981 tttcgctcga aagcttcctg tcagatccat agcgaatcaa gtgctgaatg tcacagtatc
     2041 gaacagaaaa cagtgacgat ctaacccttc aagaatattc tacgattgtt ctgtttagga
     2101 aaagcaaggc gggaagtcgg gagataagtc attgataaag tggcggagag agggggattt
     2161 gaacccccgg tagagttgcc cctactccgg ttttcgagac cggtccgttc agccgctccg
     2221 gcatctctcc gttcagatgg ttgccatgat gccaggaaat ttggcatttt aacagtccct
     2281 gtccgtgcaa ttttgttcaa gtgacgagtt tgcgagcaaa acgatgatta agtggccctg
     2341 gaaagtacaa gaatcagcac atcaaactgc ccttccctgg caggaagcac tatcgatccc
     2401 ccttttaacg tgtctgacgg aacaggaaca aagcaaatta gtcactcttg ccgaacgttt
     2461 tttacagcaa aagcggcttg ttcctttaca gggctttgaa ctggattcat taagaagctg
     2521 ccggatagca cttctatttt gcctacccgt tctggagtta ggactggaat ggctggatgg
     2581 ttttcatgaa gtcttaattt atcctgcgcc atttgtggtc gatgatgaat gggaagacga
     2641 tatcggtctg gtgcataacc aacgtattgt tcagtcaggt cagagctggc agcaagggcc
     2701 tatcgttttg aactggttgg atatacaaga ttcttttgat gcttctggtt ttaacctgat
     2761 tattcatgaa gtcgctcata agctggacac ccgtaacggc gatcgcgcca gcggagttcc
     2821 ctttattccg ttgcgtgagg ttgctggctg ggaacacgat cttcatgctg caatgaacaa
     2881 cattcaggaa gaaatcgaat tagttggcga gaatgcggcg agcattgatg cttatgctgc
     2941 cagtgatcct gctgaatgtt ttgccgtact ttctgaatat ttctttagcg ccccagaact
     3001 ttttgctcct cgtttccctt cattgtggca acgtttctgc caattttatc aacaagatcc
     3061 tttgcagaga ctgcatcgca ctaatgatac agactcgttt tcggcgacga atgttcatta
     3121 attaacaact ttgcagatta attaaccaat tgaaatgact tatgaaattt agtgttgaca
     3181 gacaaggtac cgctaagtaa tattcgcccc gttcacacga ttcctctgta gttcagtcgg
     3241 tagaacggcg gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaaat
     3301 tctaaaaatt cgcttcttag cgcaatgtca ctaaccttag ttgaacattg tttattaacg
     3361 gatagcgggt ttttaacatc ttaagcgccc tcgaccttta tggttgaggg cgttttgcta
     3421 tgaacgccat caccattttc ccctcgatta taaaacttga gttattcagt agtctcccct
     3481 cttgcaactc acacccaaaa ctgcctaacg aaaagttatt aattttcaat catattgcta
     3541 tcaggattta cattttttcg ctgtgctaga aagggcgcat ttatgttagc tcgttcaggg
     3601 aaggtaagca tggctacgaa gaagagaagt ggagaagaaa taaatgaccg acaaatctta
     3661 tgcgggatgg gaattaaact acgccgctta actgcgggta tctgcctgat aactcaactt
     3721 gcgttcccta tggctgcggc agcacaaggt gtggtaaacg ccgcaaccca acaaccagtt
     3781 cctgcacaac tcgccattgc aaatgccaat acggtgccct acacccttgg agcgctggaa
     3841 atcggcccaa agcgttgccg aacgtttcgg tatttcggtg gctgagttac gcaaactcaa
     3901 ccagtttcgt acgtttgctc gaggttttga taatgtccgc cagggtgatg aactggatgt
     3961 cccggcacaa gttagtgaaa ataatttaac cccgccaccg ggtaatagca gcggcaacct
     4021 tgagcaacag atagccagta cttcacagca aatcgggtct ctgctcgccg aggatatgaa
     4081 cagcgagcaa gcggcaaata tggcgcgtgg atgggcctct tctcaggctt caggcgcaat
     4141 gacagactgg ttaagccgct tcggtaccgc aagaatcacg ctgggcgtgg atgaagattt
     4201 tagcctgaag aactcccagt tcgattttct ccatccgtgg tatgaaacgc ctgataatct
     4261 ctttttcagt cagcatactc tccatcgtac tgacgagcgt acacagatta acaacggctt
     4321 aggttggcgt catttcactc ccacatggat gtcgggcatc aacttctttt tcgaccacga
     4381 tcttagccgt taccactccc gcgccggcat tggcgcggag tactggcgcg actatctaaa
     4441 attaagcagt aacggctatt tgcgactgac caactggcgc agcgcacctg aactggacaa
     4501 cgattatgaa gcacgcccgg ccaatggctg ggatgtacgc gcagaaggct ggctacccgc
     4561 ctggccgtac cttggcggta aactggtcta tgaacagtat tatggcgatg aagtggccct
     4621 gttcgataaa gacgatcggc aaagtaatcc tcatgccata accgctggac ttaactatac
     4681 ccccttcccg ctaatgacct tcagcgcgga gcaacgccag ggtaaacagg gcgaaaatga
     4741 cacccgtttt gccgtcgatt ttacctggca acctggcagc gcaatgcaga aacagcttga
     4801 cccgaatgaa gtcgctgcac ggcgtaacct tgcaggcagc cgttatgatc tggtggatcg
     4861 caacaacaac atcgttctgg aatatcgcaa aaaagaactg gtccgcctga ccctgacaga
     4921 ccccgtgaca gggaagtcag gagaagtgaa atcactggtt tcgtcgctac aaaccaaata
     4981 tgccctgaaa ggctataacg tcgaagccac cgctctggaa gctgccggtg gtaaagtggt
     5041 tacaacgggt aaagatattc tggttaccct gccggcgtac cggttcacca gtacgccaga
     5101 aaccgataac acctggccga ttgaagtcac cgctgaagat gtcaaaggca atttgttcga
     5161 atcgtgaaca gagcatggta gtcgttcagg cacctacgtt aagccagaaa gattcctcgg
     5221 tatcgttaag tacccagacg ttgagcgcgg attcccattc aaccgccaca ctgactttta
     5281 ttgcgcatga tgcagcaggt aatcctgtta tcgggctggt gctctcgacg cgtcacgaag
     5341 gtgttcagga catcaccctt tctgactgga aagataatgg tgacggaagc tatacccaga
     5401 tcctgaccac aggtgcgacg tctggcacgc tgacgcttat gccacagctg aacggtgtgg
     5461 atgcggctaa agcccccgcc gtggtgaata tcatttctgt ttcgtcatcc cggactcact
     5521 cgtcaattaa gattgataag gaccgttatc tctccggcaa tcctatcgag gtaacggtag
     5581 aactgagaga tgaaaatgac aaacctgtta aggagcaaaa acagcaactg aataccgcag
     5641 tcagcatcga caatgtgaaa cctggtgtca ctacagactg gaaagaaacc gcagatggcg
     5701 tctataaggc aacctatacc gcctatacca aaggcagtgg gcttactgcg aagctattaa
     5761 tgcaaaactg gaatgaagat ttgcataccg ctggatttat catcgacgcc aacccgcagt
     5821 cagcgaaaat tgcgacatta tctgccagca ataatggtgt gctcgccaat gagaatgcag
     5881 caaacaccgt ctcggtcaat gtcgctgatg aaggaagcaa cccaatcaat gatcataccg
     5941 tcacgtttgc ggtattaagc ggatcggcaa cttcctttaa caatcaaaac accgcaaaaa
     6001 cggatgttaa tggtctggcg actattgatc tgaaaagtag taagcaggaa gacaacacgg
     6061 ttgaagtcac ccttgaaaat ggcgtgaaac aaacgttaat cgtcagtttt gtcggcgact
     6121 cgagtaccgc acaggttgat ctgcagaagt cgaaaaatga agtggtcgct gacggcaatg
     6181 acagcgccac aatgaccgcg acagttcggg atgcaaaagg caacctgctc aatgacgtca
     6241 aggtcacctt caatgttaat tcagcagcag cgaaactgag ccaaaccgaa gtgaatagcc
     6301 acgacgggat cgccacagct acgctgacca gtttgaaaaa tggtgattat acggttacgg
     6361 cctctgtgag ctctggttct caggctaatc aacaggtgaa ttttatcggt gatcaaagta
     6421 ctgcagccct gaccctcagt gtgccttcag gtgatatcac tgtcaccaac acagctccgc
     6481 aacatatgac tgcaaccttg caggataaaa atggcaaccc actaaaagat aaagaaatca
     6541 ccttctctgt gccaaacgac gtcgcaagtc ggttctcgat tagcaacggc ggaaaaggca
     6601 tgacggatag taacggggtt gcaatcgcct ccctgaccgg cacgttagcg ggcacgcata
     6661 tgatcacggc tcgtctggct aacagcaatg tcagcgatgc acagccaatg acgtttgtgg
     6721 cggataaaga cagagcggtt gtcgttctac aaacatcgaa agcggaaatc attgggaatg
     6781 gcgtggatga gacgaccctg acagcaacag tgaaagatcc ttttgataac gtggttaaaa
     6841 atctgtcagt agtcttccgc acctcccccg cagacacgca actgagtctg aacgcgcgta
     6901 atactaatga gaacggtatt gctgaagtta cccttaaggg cacggttttg ggtgttcata
     6961 cagccgaagc catactgctt aacggcaaca gagatacgaa gatagtcaat attgcgcccg
     7021 atgccagcaa cgcgcaggtc accctgaaca tccctgcaca acaggtggtg acgaataaca
     7081 gcgacagcgt gcagctgacg gcgacggtga aagatccgtc gaatcatccg gtggcgggaa
     7141 taacggtgaa cttcaccata ccacaggacg tggcggcaaa ctttaccctt gaaaataacg
     7201 gtattgccat cactcaggcc aatggcgaag cgcatgtcac cctcaaaggc aaaaaagcgg
     7261 gcacgcatac ggttaccgca acgctgggta acaataatgc cagcgatgcg caaccagtca
     7321 ccttcgtggc ggataaggac agcgcggttg tcgttctgca aacatcgaaa gcggaaatca
     7381 ttgggaatgg cgtagatgag acgactctga cggcaacagt gaaagatcct tttgataacg
     7441 cagtaaaaga tctacaggtc accttcagta ccaaccccgc agatactcaa cttagtcaga
     7501 gcaaaagcaa tactaacgac agtggtgtgg ccgaagttac ctttaagggc acggttttgg
     7561 gtgttcatac agccgaagcc acactgccta acggcaacaa cgatacgaag atagtcaata
     7621 ttgcgcccga tgccagcaac gcgcaggtta cgctgaacat ccctgctcaa caggtggtga
     7681 cgaataacag cgacagcgtg cagctgacgg cgacggtgaa agatccgtcg aatcatccgg
     7741 tggcgggaat aacggtgaat ttcaccatgc cacaggacgt ggcggcaaac tttaccctcg
     7801 aaaataacgg tattgccatc acccaggcca atggggaagc gcatgtcacg ctcaaaggta
     7861 aaaaagcggg tacgcatacg gttaccgcaa cgctgagtaa taacaatacc agtgattcac
     7921 agccggtaac gtttgtggcg gacaaaacct cggctctggt tgttcttcag atatcaaaaa
     7981 atgagatcac aggtaatggc gtcgatagcg caacgctaac tgcaacggtc aaagatcagt
     8041 tcgacaatga ggtgaacaat cttccggtaa cattcagcac agcttcttca ggcctcaccc
     8101 tgaccccagg ggaaagtaat accaatgagt ctggcatcgc gcaggccact ctcgcaggcg
     8161 ttgcctttgg tgagcagact gttactgcat cactggctaa tactggtgcc agcgacaaca
     8221 aaactgtgca ctttattggc gacacaacgg cggcaaaaat tatcgagttg acgcctgacc
     8281 caggcagcat aatcgccggt accccacaga acagcaccgg tagcgtaatc accgctacag
     8341 tcattgataa taatggcttt ccggtgaaag gtgtgactgt aaacttcacc agcaacgcag
     8401 cgacagccga aatgacgaat ggcggccaag ccgtaacgaa cgaacagggt aaggctaccg
     8461 tcacttatac caatacccgc tcctcgatag aatcaggagc gagaccggat accgttgagg
     8521 ccagtctgga aaatggtagc tccacgctta gcacatcaat taatgtcaac gctgatgcgt
     8581 ctacagcaca tctcaccttg ttacaggcac ttcttgatac agtctccgca ggcgacacta
     8641 ccaatctgta tattgaggtg aaggataatt acggcaacgg tgtaccccag caggaggtac
     8701 cctcagcgtt tcaccaagtg aaggcgtgac tcccagtaat aacgctatat atacgaccaa
     8761 tcacgacggc aatttttacg caagctttac cgctacaaaa gccggggttt atcaagtgac
     8821 ggcaaccctc gaaaatggcg attcgataca acaaacggtg acctatgtgc cgaacgtcgc
     8881 gaatgccgaa atcacgctgg cagcctcgaa ggatccggtg attgccgaca ataacgatct
     8941 cacgacacta acagcaacag tcgctgatac agagggcaat gcgatagcca acactgaagt
     9001 aacatttact ctgccggaag atgtgaaggc gaacttcacg ctgagcgatg gcggtaaagc
     9061 ggttactgat actgaaggca aagcgaaagt cacgctgaaa ggtacaaaag caggcgctca
     9121 tactgttaca gcatcgatgg ctggcggtaa gagtgagcag ttggtggtga attttattgc
     9181 ggatacgctc acagcgcagg ttaatcttaa cgttaccgag gacaacttta tcgctaataa
     9241 cgtcgggatg accaggctgc aggcaacagt gactgatgga aacggcaacc cgttagccaa
     9301 tgaggcggtg acattcacgc taccggcaga tgtgagcgca agctttactc tcggacaagg
     9361 cggttccgcc attactgaca tcaacggcaa ggctgaagtt acactgagcg gtacaaaatc
     9421 cggcacctac cccgtgacag ttagcgtgaa caattatggt gtcagtgata cgaaacaggt
     9481 gactttgatt gccgatgctg gtaccgcaaa actagcctcc ttaacctctg tatactcatt
     9541 cgtcgtcagc acgaccgagg gcgcgaccat gactgcaagc gtcactgacg ctaacggcaa
     9601 cccggtagaa ggcataaaag ttagtttccg cggaacctcc gtcacgctaa gcagcaccag
     9661 cgttgaaacg gatgatcggg gtttcgctga aattcttgtg acaagcaccg aggtcggact
     9721 gaaaacagtt tcagcctctc tggcagataa acctactgaa gtcatctcgc gattactgaa
     9781 tgcaaaagca gatattaatt ctgcaacgat taccagtctg gagatacctg aaggtcaggt
     9841 catggtcgca caagacgtag cagttaaagc tcacgtcaac gaccagtttg gcaatccgat
     9901 tcttaatgaa tctgtaacat tcagtgcaga gccaccagag cacatgacca tcagccaaaa
     9961 tattgtctct actgatacgc atggtatagc cgaggtcact atgacgcccg aaagaaacgg
    10021 ttcgtatatg gtgaaagcat ccctggcgaa tggatcctct tatgagaagg atctggtggt
    10081 aatcgatcaa aaactgacac tctcggcgtc cagcccgctt atcggtgtca attccccaac
    10141 aggtgcaact ctgacggcaa cgctaacttc tgcaaatggc actccagtgg agggtcaggt
    10201 catcaacttt agcgtaacgc cagaaggtgc gacgttaagt ggcggaaaag tgagaaccaa
    10261 ctcttcaggt caggctccag tcgttctgac cagcaataaa gtcggtacat atacggtgac
    10321 tgcatcgttc cataacggtg taacaataca gacacagaca atcgtgaaag tcactggcaa
    10381 ctcaagcacc gcccatgttg ctagctttat cgctgctcca tcgactatcg ccgccaccaa
    10441 cagtgattta agtaccttaa aggcaacggt tgaggatggc agtggtaacc tgatcgaagg
    10501 tttaactgtg tacttcgcct taaaaagcgg ctctgccaca ttaacgtcat taacagcggt
    10561 gaccgatcaa aacggaatcg cgacaacaag cgtgaaagga gcgatgacag gtagcgtcac
    10621 ggtaagcgca gtcacgaccg ctggtggaat gcaaacagta gatataacgc tggtggctgg
    10681 cccggcagac acctcgcagt ccgtccttaa gaacaatcgg tcatcattga aaggagactt
    10741 taccgatagt gctgagctac atcttgttct gcacgatata tcaggccatc cgatcaaagt
    10801 ttctgaaggg ctggaatttg tgcagtcagg taccaacgtg ccctatgtgc aagttagtgc
    10861 aattgactac agtaaaaatt tctcaggcga gtacaaagcc actgttacag gcggcggaga
    10921 gggtatcgca acgctgatcc ctgtattgaa tggtgttcat caagctggtc tgagtaccac
    10981 aatacaattc actcgcgcag aagacaaaat aatgagcggt acagtgtcag tcaatggtac
    11041 tgacctaccg acaactacat tcccttcgca gggattcacc ggggcgtatt atcagttgaa
    11101 taatgacaac tttgcccctg gaaaaacggc ggctgattat gagttttcaa gctctgcctc
    11161 ctgggtcgat gttgatgcta ccggtaaagt gacatataaa aatgtcggca gcaattggga
    11221 aaggattacg gcgacgccaa aatcaggagg ccctagctat gtatacgaaa tccgtgtgaa
    11281 gagttggtgg gtgaacgccg gcgatgcttt catgatatac agccttgctg aaaatttttg
    11341 cagtagcaat ggctacacac ttccccgtgc agatcattta aaccatagtc gttcccgagg
    11401 catcgggtca ctgtacagtg aatggggaga tatggggcat tacacgactg aggctggttt
    11461 tcaatcaaat atgtattggt catctagtcc cgcaaactca aacgaacaat acgtagtttc
    11521 cctggcaaca ggtgatcaaa gcgtatttga aaagcttggg tttgcttatg cgacatgtta
    11581 taaaaacctc tgattttccg ttataatttc ttaaagagtg ctccattcgg ggcactcttt
    11641 tttacattcc ctctatagag cttgctcttc tctcaccgaa aatgaataaa taaaaatgcg
    11701 ataccgccag attcgcgttc ggtgtgacta tgatcgtatc cggtgagtac agctttgatc
    11761 caaaggctgg ttttctgtga tgattgacca gcctcagagt attgatgaca ccaccgttca
    11821 taacagatcg gcattactat gcataagtac tgtctcatat tctcgccaaa acacttttta
    11881 atgcctttgc gaattcatcc agacttatat tgtcaatttt ttgcccaacg acgactgact
    11941 ccgaaatatc ggccattgcg ggccatcgcg ttttgtaatc agcataaatt gcaacaacgg
    12001 gggtattata ggcacttgcc atatgtaata ttgaagtgtc aggagttatt gcgatataag
    12061 catcttttat gattgctgct gaacgtttaa tcgagggtga taaggataaa cggtagacat
    12121 tattataaca atccactaat accctggctt tatcttcccc ttttggtcca tgaacgatca
    12181 ctattggtat atctgtttca ctttgaattt ttgctattag attttctgca atcgatagtg
    12241 aaaatgtacg ttcctgcgag ctaccttcta aattaagcgc aatgtaagat cctaacgatc
    12301 gcatttcctc gcgcacctca tcgagtacat cctcacttag tggcaattca tatattggcc
    12361 tgactgctgg aaaacccgcc tcacgcatca taaatgccca tgtcatgggt acgggagccc
    12421 ggagattctg atccatccgg gacgcgttct tacacacagg ggaatagcac ttcatggtta
    12481 aatcaacaac ctgaaaattc gtttttgctt tcaactgact gataaataac atcgttttca
    12541 ggttcttttt acgcatcgcc tctatacaaa gatccggcgt accgtattgc tgtgttatgt
    12601 tcttcgctaa atcttttatt tcttttaatg ttgcgtgatc ctgcatagtc attgtgacta
    12661 atgttaattt agtctgttca agtttgagca cattaaagac ttctaaatta attgtcgacg
    12721 taacaattaa tagatgctta attttatgca attcaagcgc ccgaataaca ggaaagatgg
    12781 ccatagcatc gccaatctga tcgggaatat ggatgacaac aaagtctgtt ttttcaatat
    12841 tgaaattata agctttataa tcgtagtagc taaatgcaat acgtctcaac aatgatacta
    12901 aaaacataac taacctcgcc tccctactgg ttataatgca atgcagtcta tcagaatcat
    12961 cagggtgcca ttttgtgcat atgcggactt ttatgtttca tatctcgaac ctgtgggtcc
    13021 tctgcttaat ccttaaacaa caccaacaac tcccgcgctt tcatcttcca gcgaattttt
    13081 catgttgccg ctaatcagcc ataaaatcat ttgcagatgc gctctgtcga ggtagtctca
    13141 taaggttcgt ttatagatcg acggcaatgt gagttacctt ttccatacta attataaaaa
    13201 gacagtacaa acaggatcat tatggactcc acgctcatct ccactcgtcc cgatgaaagg
    13261 acggtttcgt taagtcgcgc ccgacgagct gcgttaggca gtttcgctgg tgccgtcgtc
    13321 gactggtatg attttttact ctatggcatc accgccgcac tggtgtttaa tcgcgagttt
    13381 ttcccgcaag taagcccggc gatgggaacg ctcgccgcat ttgctacctt tggcgtcgga
    13441 ttccttttcc gtccgctcgg cggtgtcatt ttcggtcact ttggcgaccg actgggacgt
    13501 aagcgcatgt taatgctgac tgtctggatg atgggcatcg cgacagcctt gattggtatt
    13561 cttccttcat tctcgaccat tgggtggtgg gcacctattt tactggtgac gctgcgtgcc
    13621 attcagggat ttgccgtcgg cggcgaatgg ggaggcgcgg cgttgctttc cgttgaaagt
    13681 gcaccgaaaa ataaaaaagc cttttacagt agcggtgtac aagttggcta cggtgtaggt
    13741 ttactgcttt caaccggact ggtttcattg atcagtatga tgacgactga cgaacagttt
    13801 ttaagttggg gctggcgtat tcctttcctg tttagcatcg tactggtact gggagcattg
    13861 tgggtgcgca atggcatgga ggagtccgcg gaattcgaac aacagcaaca taatcaagct
    13921 gccgcgaaaa aacgcatccc ggttatcgaa gcgctgttac gacatccagg tgctttcctg
    13981 aagattattg cgctacggct gtgcgagttg ctgacgatgt acatcgttac cgcctttgca
    14041 cttaattatt caacccagaa tatggggtta ccgcgcgaac ttttccttaa tattggtttg
    14101 ctggtaggtg gattaagctg cctgacaatt ccctgttttg cctggcttgc cgatcgtttt
    14161 ggtcgccgca gggtttatat cacaggtgcg ttaatcggaa cgttgagcgc atttcctttc
    14221 tttatggcgc ttgaagcaca atctattttc tggatagttt tcttctccat aatgctggca
    14281 aacattgcgc atgacatggt ggtgtgtgtg caacaaccga tgtttaccga aatgtttggt
    14341 gccagttatc gctatagtgg cgctggagtc ggttatcagg ttgccagtgt ggttggcgga
    14401 ggatttacac cttttattgc cgctgcactc atcacttact ttgccgggaa ctggcatagc
    14461 gtcgccatgt atttgctggc tggatgtctg atttctgcaa taaccgcttt gttgatgaaa
    14521 gacagtcaac gcgcttgata gcctggcgaa gatcatccga tcttcgcctt acacttttgt
    14581 ttcacatttc tgtgacatac tatcggatgt gcggtaattg tatggaacag gagacacaca
    14641 tgaataataa gggctccggt ctgaccccag ctcaggcact ggataaactc gacgcgctgt
    14701 atgagcaatc tgtagtcgca ttacgcaacg ccattggcaa ctatattaca agtggcgaat
    14761 tacctgatga aaacgcccgc aaacaaggtc tttttgtcta tccatcactg accgtaacct
    14821 gggacggtag cacaaccaat ccccccaaaa cgcgcgcatt tggtcgtttt actcacgcag
    14881 gcagctacac caccacgatt actcgcccta ctctctttcg ttcgtatctt aatgaacaac
    14941 ttacgttgct gtatcaggat tatggtgcgc atatctcggt gcaaccctcg cagcatgaaa
    15001 tcccttatcc ttatgtcatc gatggctctg aattgacact tgatcgctca atgagcgctg
    15061 ggttaactcg ctatttccct acaacagaac tggcgcaaat tggcgatgaa actgcagacg
    15121 gcatttatca cccaactgaa ttctccccgc tatcgcattt tgatgcgcgc cgcgtcgatt
    15181 tttccctcgc gcggttgcgc cattataccg gtacgccagt tgaacatttt cagccgttcg
    15241 tcttgtttac caactacaca cgttatgtgg atgaattcgt tcgttgggga tgcagccaga
    15301 tcctcgatcc tgatagtccc tacattgccc tttcttgtgc tggcgggaac tggatcaccg
    15361 ccgaaaccga agcgccagaa gaagccattt ccgaccttgc atggaaaaaa caccagatgc
    15421 ccgcgtggca tttaattacc gccgatggtc agggtattac tctggtgaat attggcgtgg
    15481 gaccgtcaaa tgctaaaacc atctgcgatc atctggcagt actacgcccg gatgtctggt
    15541 tgatgattgg tcactgtggc ggattacgtg aaagtcaggc cattggcgat tatgtacttg
    15601 cacacgctta tttacgcgat gaccacgttc ttgatgcggt tctgccgccc gatattccta
    15661 ttccgagcat tgctgaagtg caacgtgcgc tttatgacgc caccaagctg gtgagcggca
    15721 ggcccggtga ggaagttaaa cagcggctac gtactggcac tgtagtaacc actgatgaca
    15781 ggaactggga attacgttac tcagcttctg cacttcgttt taacttaagc cgggccgtag
    15841 caattgatat ggaaagtgca accattgccg cgcaaggata tcgtttccgc gtgccatacg
    15901 ggacactact gtgtgtttca gataaaccgt tgcatggcga gattaaactt cccggccagg
    15961 ctaaccgttt ttatgaaggt gctatttccg aacacctgca aattggcatt cgggcgatcg
    16021 atttgctgcg cgcagaaggc gaccgactgc attcacgtaa attacgaacc tttaatgagc
    16081 cgccgttccg ataataagtt atgaaccttc ttatcaaaaa tagatgtgaa ggattaagta
    16141 agacatctgg caaatagccc gcaataaacg cgggctgttt gcttccttgg gcggatacga
    16201 gttttattat cgtcttaatg atttccacat attaaaagca agtatgcttt caaaacacaa
    16261 ttataaaaaa tcccaccaac aatataagtt tatat
//
LOCUS       DACBVI010000101        15965 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.101, whole genome shotgun sequence.
ACCESSION   DACBVI010000101 DACBVI010000000
VERSION     DACBVI010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.101"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..620
                     /locus_tag="IU325_15715"
     CDS             <1..620
                     /locus_tag="IU325_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP5810450.1"
                     /translation="LYGTRISVLFGLMLTLCSSVMGVLAGALQGYYGGKVDLWGQRFI
                     EVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSLVGVVRAEFLRTRNFDYIRAA
                     QALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTLTSLDFLGFGLPLGSPSLGEL
                     LLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDAFDPNKAV"
     gene            622..2211
                     /gene="yejF"
                     /locus_tag="IU325_15720"
     CDS             622..2211
                     /gene="yejF"
                     /locus_tag="IU325_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HAP5810451.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDNTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(2215..2559)
                     /locus_tag="IU325_15725"
     CDS             complement(2215..2559)
                     /locus_tag="IU325_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810452.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(2892..4082)
                     /gene="bcr"
                     /locus_tag="IU325_15730"
     CDS             complement(2892..4082)
                     /gene="bcr"
                     /locus_tag="IU325_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HAP5810453.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(4110..4805)
                     /gene="rsuA"
                     /locus_tag="IU325_15735"
     CDS             complement(4110..4805)
                     /gene="rsuA"
                     /locus_tag="IU325_15735"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HAP5810454.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            4955..6715
                     /locus_tag="IU325_15740"
     CDS             4955..6715
                     /locus_tag="IU325_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP5810455.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            6840..7124
                     /gene="rplY"
                     /locus_tag="IU325_15745"
     CDS             6840..7124
                     /gene="rplY"
                     /locus_tag="IU325_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HAP5810456.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAVELDH
                     DKVMNMQVKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLLHIDFVRA"
     gene            complement(7263..8270)
                     /gene="yejK"
                     /locus_tag="IU325_15750"
     CDS             complement(7263..8270)
                     /gene="yejK"
                     /locus_tag="IU325_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HAP5810457.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            8452..8679
                     /locus_tag="IU325_15755"
     CDS             8452..8679
                     /locus_tag="IU325_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1414 domain-containing protein"
                     /protein_id="HAP5810458.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            8699..10459
                     /gene="pbgA"
                     /locus_tag="IU325_15760"
     CDS             8699..10459
                     /gene="pbgA"
                     /locus_tag="IU325_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HAP5810459.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAASNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNSQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            10534..10610
                     /locus_tag="IU325_15765"
     tRNA            10534..10610
                     /locus_tag="IU325_15765"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10568..10570,aa:Pro,seq:ggg)
     gene            complement(10712..13303)
                     /gene="yejO"
                     /locus_tag="IU325_15770"
     CDS             complement(10712..13303)
                     /gene="yejO"
                     /locus_tag="IU325_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HAP5810460.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGISSNTEINGGYQYIEMNGTAEYSILNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANEPTIKGGRLIIEKDGITVFAAIEKGGLLEVKEGG
                     LAFAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKDGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTRVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSDSAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTRGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNIQ
                     AGVVWTF"
     gene            13623..14270
                     /gene="narP"
                     /locus_tag="IU325_15775"
     CDS             13623..14270
                     /gene="narP"
                     /locus_tag="IU325_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HAP5810461.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(14305..15357)
                     /locus_tag="IU325_15780"
     CDS             complement(14305..15357)
                     /locus_tag="IU325_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="cytochrome c-type biogenesis protein; involved in
                     the attachment of heme to cytochrome c; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcmH"
                     /protein_id="HAP5810462.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGFAVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGLALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(15354..15911)
                     /gene="dsbE"
                     /locus_tag="IU325_15785"
     CDS             complement(15354..15911)
                     /gene="dsbE"
                     /locus_tag="IU325_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HAP5810463.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
BASE COUNT         3918 a         4257 c         3864 g         3926 t
ORIGIN      
        1 ttctctatgg cacgcggatc tcagttctgt ttggcctgat gctgaccctc tgctccagcg
       61 tgatgggcgt gctggcgggg gcgctacaag gctattacgg cggtaaagtc gatctctggg
      121 ggcaacgttt tattgaagta tggtcgggga tgccgacgct gtttttgatt attttacttt
      181 ccagcgtcgt acagcctaac ttctggtggc tgttggcaat taccgtcttg tttggctgga
      241 tgagtctggt cggcgtggtg cgggcggagt ttttacgtac ccgtaatttc gactacatcc
      301 gcgcggcgca ggcgcttggc gtcagcgatc gcagtatcat cctgcgtcat atgttgccaa
      361 atgccatggt cgctaccctc acctttttac cgtttatttt atgtagttcg atcaccaccc
      421 tgacctcact cgatttcctc ggcttcggtc tgccgctcgg ttcaccgtca ctcggcgaac
      481 tgctgttaca agggaaaaat aaccttcagg ccccgtggct tgggatcacc gccttcttgt
      541 cggtggcgat attgttgtct ttgctgatct ttattggtga agccgtccgc gacgcatttg
      601 atcctaataa ggcggtgtag catgacgcaa actctgttag cgattgaaaa tttgtcggtg
      661 ggttttcgcc atcagcaaac cgtacgtaca gtagtcaatg atgtttcact tcagattgag
      721 gctggcgaaa cgctggcgct ggtgggtgag tcaggttcag gcaaaagcgt taccgcgctg
      781 tcaattttac gcctgctccc ttccccgccg gttgaatatc tctccggcga tattcgtttt
      841 catggcgaat cgctgcttca tgccagcgat caaacgttgc gcggtgtacg cggtaataag
      901 atcgccatga tttttcagga gccgatggtg tcattaaatc cattgcatac cctggaaaaa
      961 cagctttatg aagtgctttc actccaccgc gggatgcgtc gggaagcggc tcgtggcgaa
     1021 attcttaact gccttgatcg cgtcggtatc cgccaggcgg caaaacggct gacagattat
     1081 ccgcatcagc tctccggcgg cgaacgccag cgggtgatga ttgcgatggc gctgttaacg
     1141 cgacctgaat tattaattgc cgatgaacca accaccgcac tggacgtctc tgttcaggcg
     1201 caaattttac agctgttgcg cgaactgcaa ggcgagctga atatgggtat gctgtttatt
     1261 actcataacc tcagcattgt cagaaaactg gcccatcgcg tggcggtaat gcaaaacggt
     1321 cgctgtgtcg agcaaaataa cgccgctacg ctatttgcct cacccactca tccttacaca
     1381 caaaagctac tcaacagtga accgtcaggc gacccggtac cattgccaga acctgcctca
     1441 acgttgctgg atgttgaaca gcttcaggtt gccttcccca ttcgcaaagg gattttgaag
     1501 cgcattgtgg atcataatgt ggtggtgaaa aacatcagtt ttacgctacg agcgggtgaa
     1561 acactgggtt tagtaggcga gtcaggttcc gggaaaagca cgacgggact ggcgctgctg
     1621 cgactgatta actctcaggg cagcattatc tttgacggtc agccactgca aaatttaaat
     1681 cgtcgccagc tgttacctat tcgtcatcgc attcaggtgg tatttcagga tccaaactcc
     1741 tcgctcaacc cacgactcaa cgttttgcag attattgagg aaggcttacg ggttcaccag
     1801 ccgacgcttt ctgccgcaca acgcgaacaa caagtgatag ccgtgatgca tgaagtggga
     1861 ttagatcctg aaacacgcca ccgttatccg gcggagttct ctggtggtca gcggcaacgt
     1921 attgcgattg ccagggcgtt aattcttaag ccctcgctga tcattcttga tgaaccaaca
     1981 tcatcgctcg acaacacggt acaggcgcaa atattgacgc tattgaaatc attgcaacaa
     2041 aagcatcaac tggcctattt gtttatcagt cacgatttgc acgttgtccg cgcgttatgt
     2101 catcaggtta tcgtactgcg acaaggggaa gtagtggaac aaggaccgtg cgcgcgcgtg
     2161 tttgccgcac cgcagcagga gtatacgcgt cagctactgg cgttgagctg acgcttaaaa
     2221 aggattgtag tttgagaagg gttcggcaat tgccacacca aaatttttca atcggcatgt
     2281 tgcagcaaac tcgtcctggc gatttacaaa caggcacggc tcaccttcac actccagtac
     2341 cgaacatgga acttcgatgt cgcttaacgc ctggctgagt ttgtacatca ccgaccatgc
     2401 attatcacca tccgggctaa gcaatttaag cgccaccagg ctgttgtcac cgcacggtcc
     2461 attttgcgga atcggttcaa ccttcgaacc tgcgaaacca gcagaccagc gatagttctg
     2521 cggcagtcga tggacgattg agagttgtaa tgaagtcact tattttcccc ggaagcacat
     2581 ttacttcaca atttgtttac atcaatttta acacatcatc aacaatccgt ctttatacag
     2641 atcgtaaaga ttgatgcctc gccagcgtgg cctctatggc tctgatttaa ataatatctg
     2701 cgggcgtggt cacgtttgcg gggctatatg tacccgtttc tgcgatctaa ctcaaccttt
     2761 ttaactacaa tgatgtgact ttttacataa attgatttta cataaaataa acatatatcg
     2821 ggggaatgat ggatttgtgg ttgacatgca tcaacaaagg aagcctttta gcttcctcgt
     2881 tgtgcaatag atcaccgttt tttcggccga ctggcgtaca gacagaagag aatggagctg
     2941 gttgcgcaga atgcaattga ccaaatcatc ggccatgcag agttaaaggt cgcaagagaa
     3001 agtaatgcgc caacaattgc ccctatgccg aaacggaagg ttcctgccag cgaagatgcc
     3061 gttcccgcca tatggggaaa ctcatcaaga atgaccgcca tcgcattaga tgacaccatc
     3121 gacacgcagc ccacaaacgc cgcaacgcca accaccagcg accagaatcc cagccccagc
     3181 agcgcactga tgaccatcca cgccgccata ataaactgta tccacaaccc cgagcggaac
     3241 atatttaacg cgccaattcg gcggacgaag cggctgttaa agatagtcat cacgaacagg
     3301 aaaacaatgt ttagcgcaaa gtaataacca aagttttccg gcgcgacgtg gttaatttca
     3361 atgtagacaa acggtccggc gcttaagaac gagaacatcc cggcaaagct gaaaccactg
     3421 gcaagcatat aactcaggac acgcttatga cggaacagcg ccgcaaagtt accaatagtg
     3481 gtacgaatgt gaaatggctg acgacgctct ggcggcaacg tttctttaat caggaagaaa
     3541 atcattgccg aagccagaat cgccgctaat gccaggatcc agaagatgta atgccagctc
     3601 agccacacca gcacccaacc gccgacaatc ggtgccatca acggtgcaat agtggtcacc
     3661 agcatgacaa acgacatcat ccgcgagaac tcttctttcg ggtaaatatc gcgcatcagg
     3721 gcattaatga ccacgctggc cgcagccgca gccagcccgt ggaagaaacg catcacaatc
     3781 agctgatcga tggtgtttgc caacgcacac gccaccgcgg cggcggcaaa caccagcgta
     3841 ccgccaagaa ccaccggctt acgcccgaag ctgtctgcca tcggcccgta gattaactgc
     3901 cccaacgcaa agcccagaat ataagtactg agggtcatct gcgtactgcc tgccggtacg
     3961 ccaaactgcg ctgaaattac cggtagcgcg ggcagataca tatcaatcga cagcggcatc
     4021 aacatggcca gcaggccaag gataaaaaca atagcaaacg acgaatgctg tcgggtggtc
     4081 acaacgggct cctgaaagtc attgaaaagt tagacgacgc tggcaatttc ttcttcagtt
     4141 aacggacgat attcaccggg agctaaatca gcatccagcg taatgccgcc aatacgttca
     4201 cgatgcagct caaccacatg gttacccacg gcggcgaaca tacgtttcac ctgatgataa
     4261 cgcccttcgc tgatggtcaa acgaacctgc gttggggtaa tcacttccag caccgccggc
     4321 ttagtgagat ctttttcgtt atgcagctgc acgcctttag caaattgctc tgccgtatcg
     4381 tcagctacgg gtgattccag cgtcaccaga taggtcttct cgcaatgatg gcgcggagaa
     4441 gtaatgcggt gcgaccactg accatcatct gtcattagta ccagaccggt ggtatcgata
     4501 tccagacgcc ctgcagcgtg cagtttccac gctaccggtt catcaagaaa atagagcacc
     4561 gttgggtgat ccgggtcgtc cgtggagcaa acatagccct gaggtttatt gagcatgaag
     4621 taacgtggac cgtgttgctg cgccagcggg ttgccatcgt aagcgacatc atgttcaggg
     4681 agcagtttga acgctgcatt acggacgatt tcgccatcga cggtgacacg attgccgcgg
     4741 atttcacgcc cggcaatagc acggctaacg ccgagttgct gtgcgataaa tttatcaagt
     4801 cgcatgtgtg tgattttgcc tgtaaaaaac ggaggtcggg taatgacccg aaaatctgaa
     4861 ctgttgtctg cccagtatag cggtctaata acttccctca agggaaaaag atccgtggca
     4921 tactattagc agaataatct acctacgcga gaccatgatt tttacactcc gcccatatca
     4981 gcaagaagcc gtggatgcca cgctcaacca ttttcgtcgt cataaaaccc ctgccgttat
     5041 cgtgctgccc accggtgcag gtaaaagcct ggtgatagcg gaactggcgc ggctggcgcg
     5101 tggtcgcgtg ctggtgctgg cacacgttaa agaactggtg gcgcaaaacc atgcaaaata
     5161 tcaggcgctg gggctggaag ccgatatttt tgccgccggg ctaaagcgca aagagagcca
     5221 cggtaaagtg gtatttggca gcgtgcagtc ggtcgcccgt aatcttgatg cctttcaggg
     5281 tgaattttcg ctgttgattg tcgatgaatg tcaccgtatt ggtgacgatg aagagagcca
     5341 gtatcagcaa atcctcactc acctgacaaa agtgaatccc cacttacgcc tgctggggct
     5401 gactgccacg ccttttcgat tgggcaaagg ctggatctac cagttccatt atcacggcat
     5461 ggtacgcggc gatgagaaag cccttttccg tgactgcatt tatgagctgc cgctgcgtta
     5521 tatgattaaa cacggctatc tgacgccgcc agaacgactg gatatgccag tagtgcaata
     5581 cgatttcagc cgcttgcagg cacagagtaa cgggcttttc agcgaagccg atctcaaccg
     5641 cgagctgaaa aaacagcaac gtattacccc gcacatcatc agccagatta tggagttcgc
     5701 cgaaaagcgt aaaggagtga tgattttcgc cgccaccgtt gaacacgcca aggagatagt
     5761 gggactgctg cctgccgaag atgcagcact gattactggc gacacacccg gcgctgagcg
     5821 cgatgtgtta attgaagatt ttaaagccca gcgttttcgc tatctggtca acgtcgcggt
     5881 actgaccact ggatttgacg ccccgcacgt cgatcttatt gctattctgc gccctaccga
     5941 atcggtgagt ctttaccaac aaattgtcgg gcgcggtctg cgtctcgctc caggcaagac
     6001 tgattgctta attcttgatt atgcgggtaa ccctcacgat ctctacgcgc cggaagttgg
     6061 tacaccgaaa ggcaaaagtg acaacgttcc ggtacaggtt ttctgccctg cctgcggttt
     6121 tgccaacacc ttttggggga aaacgaccgc cgacgggaca ttgattgaac actttggtcg
     6181 ccgctgtcag ggatggtttg aagatgacga cggtcatcga gaacagtgtg atttccgttt
     6241 ccgttttaaa aattgcccgc aatgtaacgc ggaaaacgat attgccgccc gccgctgccg
     6301 cgaatgtgac accgtactgg ttgatccgga tgatatgtta aaagcggcgc tacgactgaa
     6361 agacgcgctg gtattacgct gtagcggcat gtctttgcag catgggcacg acgagaaagg
     6421 cgaatggttg aaaatcacct attacgatga agacggcgcg gatgtgagtg agcgtttccg
     6481 tctgcaaacg cccgctcagc gtaccgcctt cgagcagctt tttatccgcc cgcatacgcg
     6541 cacaccgggc atccctctgc gctggatcac cgccgctgat atcctcgccc agcaagcatt
     6601 actgcgacac ccggattttg tcgtcgcccg catgaaaggt cagtactggc aagtgcgtga
     6661 aaaagtgttc gattacgaag gtcgttttcg tcgggcgcac gaattacgcg gttaatggta
     6721 gttttgattg atgtataagg cgattgagta tagaatctcg cccgcttttg catacgcaaa
     6781 gcagatcact tacctgttgc tgggtcgcct gtagcaggat taatttaaga gagaaagaaa
     6841 tgtttactat caacgcagaa gtacgtaaag agcagggtaa gggtgcgagc cgccgcctgc
     6901 gtgccgctaa caagttcccg gcaatcatct acggtggcaa agaagcgccg ctggctgtcg
     6961 aactggatca cgacaaagtc atgaacatgc aagttaaagc tgaattctat agcgaagttc
     7021 tgaccatcgt tgttgacggt aaagaaatca aagttaaagc tcaggacgta cagcgtcacc
     7081 cgtacaaacc gaagctgctg cacatcgact tcgttcgcgc ttaattgctg aataagttgt
     7141 aaaaaacccc gctccggcgg ggttttttgt atctgcagaa tatgcctgat tacggtattg
     7201 ctattttttg caggccagat aaggcgttca cgccgcatct ggcattcaga acacaaaacc
     7261 gattaattgc cgccagacgt ccggcgctgc aattgatcgc gcaaattcgg cggcgtgcct
     7321 ttaatggtca gggtatcggt tgctggatcc cagaagatgc gttcacctaa cagcatcgca
     7381 tcaaagttaa tagtcaagcc accgccgcta ccggcaaact tcgtcaactg acgcagtgtg
     7441 ctacggtctg ccgggaagct ctcttccagt tcgtagcctt tttcagccgc aaactccgtg
     7501 aagctcactt cgctgacgcc tgccagctct ttcgacagcg attccagttc aatctcttcg
     7561 cctgcctgca gctgctcatt acagtagcta taaacctgct ggcgcacgtt ctgtcgctct
     7621 gctttatcca gttgtgcttc ggcagtgaaa tcatcaaccg cttgcaacag cccacggttt
     7681 tgcgctttcg cgtttaaccc ttcgctggcc ccgaggaaat ccataaagaa atccgccact
     7741 ttgcgcccta ctcgcccttt caggaaagtg agatagcggg tcgactctgg attggtttcc
     7801 cactcggtta aatcgatacg cgcaacaata tccgcatgat tgatatcaag ataatgggtt
     7861 gggttgatgt cgagattttc gttaacacgc atactgctca ggttgctcag aactgccacc
     7921 agcagatact ccaccgccag atagcgatag tggcaaaaca gcacgaatcc accgtcagca
     7981 aaaggatatt tcgctaattc gtcacgcagg cgaccggttg ccgcgcggct aaatgccaga
     8041 aagtcctctt cgccctgacg ctgtaagcgc agtgtctgcg ccagttcact ctcttcgcta
     8101 aacaagccgt aagctttatt tttggcgcta tagacccgat gcagttctgc caccatctca
     8161 acgacggttt ctgtcggttc cagcaatgaa tcgcgcaaca ccagctcaag gttctgctca
     8221 tcacgcttga taagctggtg cagagcaatc tggttgatat ccagactcat gataaactct
     8281 cctttaagac cgggcggtat tcaaccaccg ccggttaagc gacgcaataa aagatggcgg
     8341 tcattttaca gaaggcggcg taacgagaat gatttaacgc ataaaaaagc agaaaaaaaa
     8401 ccgttgctac ggtaatatgt tgccctttca tcaactaact gatttcgatt tatgccacaa
     8461 atttcccgct acagtgatga acaggttgaa caattgctcg cagagctgct caacgtactg
     8521 gaaaaacata aggctccgac cgacctttcc ctgatggtgt taggcaacat ggtcactaac
     8581 cttatcaaca ccagcattgc cccggcccaa cgccaggcaa ttgccaactc ttttgcccgc
     8641 gccttacagt cctctatcaa cgaagacaaa gcgcactaag ggaaacagat aacaggttat
     8701 ggtaactcat cgtcagcgct accgtgaaaa agtctcccag atggtcagtt gggggcactg
     8761 gtttgcactg ttcaatattc tgctttcgct cgtcattggc agccgttacc tgtttatcgc
     8821 cgactggccg acaacgcttg ctggtcgcat ttattcctac gtaagcatta tcggccattt
     8881 cagcttcctg gtgttcgcca cctacttgct gatcctcttc ccgctgacct ttatcgtcgg
     8941 ctcccagagg ctgatgaggt ttttgtccgt cattctggca acggcgggaa tgacgctatt
     9001 actgatcgat agcgaagtct ttactcgttt ccatctccat cttaatccca tcgtctggca
     9061 actggttatc aacccagacg aaaatgagat ggcgcgcgac tggcagctga tgttcatcag
     9121 cgtgccggtt attttattgc ttgaactggt gtttgcgacg tggagctggc aaaagctacg
     9181 cagcctgacg cgtcgtcggc gcttcgcgcg cccgctggcc gcattcttat ttatcgcctt
     9241 tatcgcctcg catgtggtgt atatctgggc cgatgccaac ttctatcgcc cgatcaccat
     9301 gcagcgcgct aacctaccgc tttcgtaccc gatgacggcg cgacgttttc ttgagaagca
     9361 tggtctgctt gatgcgcagg agtatcaacg ccgtctcatt gagcaaggta atccagacgc
     9421 cgtttccgtt cagtatccgt taagcgaact gcgctatcgc gatatgggca ccgggcagaa
     9481 tgtcttgttg attactgtcg atggcctgaa ctactcacgc ttcgagaagc agatgcctgc
     9541 gctggcaggt tttgctgagc aaaatatttc gttcacgcgc catatgagct ccggcaacac
     9601 tacagacaac ggcatctttg gcctgttcta tggcatctcg ccgagctata tggacggcat
     9661 tctgtcgacc cgtacgcctg cggcgttaat tactgcgctt aatcagcaag gctatcagct
     9721 ggggttattc tcgtcagatg gctttaccag cccgctgtat cgccaggcat tgttgtcaga
     9781 tttctcgatg ccgagcgtac gcacccaatc cgacgagcag accgccacgc agtggatcaa
     9841 ctggctgggc cgctacgcac aagaagataa ccgctggttc tcgtgggttt ctttcaatgg
     9901 cactaacatt gacgacagca atcagcaggc atttgcacgg aaatatagcc gggcggcaag
     9961 caatgtcgat gaccagatca accgcgtgct caatgcacta cgtgattctg gcaaactgga
    10021 caatacggtg gtgattatca ctgccggtcg gggtattccg ctgagcgaag aggaagaaac
    10081 ctttgactgg tcccacggtc atctgcaggt gccattagtg attcactggc caggtacgcc
    10141 ggcgcagcgt attaatgctc tgactgatca taccgatctg atgacgacgc tgatgcaacg
    10201 cctgctacat gtcagcacac ctgccagcga atattcgcaa ggtcaggatt tgttcaactc
    10261 tcaacgccgt cattactggg ttaccgccgc ggataacgat acgctggcaa ttaccacccc
    10321 gaaaaagacg ctggtgctga acaataacgg taaataccgt acttacaact tacgtggtga
    10381 aagagtgaaa gatgaaaaac cacagttaag tttgttattg caagtactga cagacgagaa
    10441 gcgttttatc gctaactgat taattataaa tcagttagca aaatatctta cttgcaatcg
    10501 gtgtggaaaa cggtagtatt aacagccacg agtcggcacg tagcgcagcc tggtagcgca
    10561 ccgtcatggg gtgtcggggg tcggaggttc aaatcctctc gtgccgacca aaaatcccca
    10621 aaaaaaccaa cctttacggt tggttttttt atatctgcat ttaattcgat aaacagaccg
    10681 cgacacatca cggcctgttt attttctgtt atcagaacgt ccagaccaca cccgcctgaa
    10741 tattccacgg cgattctacc cctgcgccat tgccataact gactgacaaa tgaccgctta
    10801 acgtcggggt aaacgatgac cttatcccag cctgataaac gccacgggtt ccagccacat
    10861 cattattaaa tttgccatcg tcattaactt tcacctggtt agaatcggcg tattcctgac
    10921 gcacggcggc tttcagccag ggttccaacg tcgtaccgtt ttgcaggtcc atgtgatagc
    10981 ttaccgccgt tcccgcttca gcgcgtaata tccgggtatt tcccacatcc gcgcgcatgc
    11041 catttgataa cgtgtagtcc tgaccatctg tggtaaagcc ggtaaaggcc agatagggtc
    11101 taacactcca caatccgtca acccaacgga acccgctctc gacatgagca cccgcgccgt
    11161 tactattgta atcgccaaac gctgttgctc cattactcat cttgccatgg atggtgttgg
    11221 caaaacggtc aactttcacc actccatcaa cataggcacc gttctgatgc tcccaaccgg
    11281 cataagcccc cagggtgtag ctatcgatat tacctttgcc gccgcgatca aaaccaatat
    11341 cagaatgaga gtaaccaaag aacaagccgc gaattgtgct gctttcttca cgggagaaac
    11401 ggctatcgat accgagcgtc aggcccgtca atgtttgctc aaagccagct cccgcatcag
    11461 tggtcacgtt gttgcgggtg ttaattgccg aactccacat cgccgtatcg taactaacgc
    11521 cttttacgct accaagacgc tcacgcacgg tgtccagttc tgcatcaaat accagcggtt
    11581 gtgctgctgc catgttcagc acatcagtgg ttgaaggggt aatttgcgcg cgattctctg
    11641 cgaggctcca gctatggttg ccattatcca gcaaggtata ttcatacgta ccgatatcaa
    11701 caacgcctcc ggcattgccc aacgtaaatg cagcatcacc gccgcccgtt gttaccagtg
    11761 taaggctatc tcctgctgcc gggctggcac cggtgtccgt cacgaatatt ttgaaatcac
    11821 ctgttgcctg accggtgacg ttgagctgat cgctctgatg attagccata tcggtacgca
    11881 tataaaaatt gccatttccc gacagggtat tggtggtcaa cgtaatgaaa ttttccgcac
    11941 ttgccgtcac agatgaacga gtcactggct cagccatcat aatcacattt gcgccagaca
    12001 tcgcaaggtt ggctaagttg aggcgagtgt tttggttcgt cgtagtgata tcggcaatga
    12061 tggtccatgc gctattttcg agcgaaacat ccgctttgct ggtatccacg gcaccatgcg
    12121 ttctaaaaga agcgctatca gaaaccacta ccttacccac caacattgcg ggcgcataat
    12181 ttatttgcgg cttcatgacc tcaagaatgc catcattccc tctgactgaa acgttaacca
    12241 ttgtgccagc ccagacgtta gcgcggccat tgttgataac catattttca gagatggctt
    12301 ttgaggaata cgtgatactt ccattctgat ctgatcgacc gagatcatat accgcatttg
    12361 cctgcactct cgtaccagta tcctttccca gcgattgcat agtggcatgc tcatcaagga
    12421 tagtatcaat ggcctgcgtg tcctccataa caatgagccc ggaaccatca tccagttcat
    12481 aattttttga cacaccatct tttatactaa attggccttt actgtttgta ccactcactt
    12541 ccagcgcatt cgttgagaca attaattttc cgcctgcttt tttatcaacg ccagttgcag
    12601 tcccgccatc cataacctcc agtaagccgc cactatccac agtggtatta taagcgaagt
    12661 cattttcttc aactcgcaaa cttccgccgt tttccaacag catattatta gcaataccat
    12721 ccttgatatc gaactgaccg agacggtttg ttccgaatac ctccatggcc cgggtggttg
    12781 ttttaatcgc accgccggct ttctgatcta ccgcaaatgc taatcccccc tctttaacct
    12841 ccagtaatcc tcccttttcg atagcggcaa agacggtaat cccatctttt tcaataatta
    12901 agcgcccgcc tttaatcgta ggttcattcg ctgcgccata aacctgtaac acaccattat
    12961 tgagggtagt ctggtttgcc gcaccaccca tttgaacaat ttgataacca tcatttaata
    13021 ttgagtattc tgcggtgcca ttcatttcaa tgtactgata cccgccgtta atttcagtat
    13081 tactacttat accaaattct ttaatatgct gttctccgcc aggattgatt ttcgtattat
    13141 tggcggtccc ataaaccagc tggatatctt tttccaggac aacaccatca actgtctcac
    13201 catactcaac tgttgatgcc aatgctatgg gtgaatataa cgctgtagcc accagaacag
    13261 atattgcgga acgacaaaga gaaacagaac cagattgatg cattgagctt tcatcctatg
    13321 aaattaattg ctgttaaaag cattgggtag agaaaatacc catagctcca tacccggagt
    13381 cagtttttaa aaactgttta aagaaatgca caagtattgt gattgatttt ttagttgttt
    13441 ttcttgatga gaagctgatg caaaattccg tctttataat gaaagtgatg ccaaagcgaa
    13501 cgacaaggtt gtagttttcg ctacatgtcc atacataaaa tggggtaaca ttcacgcgcc
    13561 tggtagcgtt accaacgcta cgctcaaaca taatgattct aataaaacct caggagacta
    13621 ctatgcctga agcaacgcct tttcaggtga tgattgtgga tgatcatcca cttatgcgac
    13681 gcggtgttcg tcagttactg gagcttgatc ctggctttga agtggtcgcc gaagcgggcg
    13741 acggcgcgag cgcgatcgat ctggcgaata gactggatat cgacgtgatc ttgctggatc
    13801 tcaatatgaa aggtatgagt ggcctggata ccctcaatgc cttgcgcagg gatggcgtta
    13861 ccgcgcaaat tattatcctg accgtatccg atgcctccag cgatgtcttt gcgctgatag
    13921 acgcaggcgc agacggttat ctgttgaaag acagcgaccc ggaagtgttg ctggaagcga
    13981 ttcgtgccgg agcgaaaggc agcaaagtct ttagcgaacg cgtcaatcag tacttacgtg
    14041 aacgtgaaat gtttggcgcg gaagaagatc ccttcagcgt gctgacggag cgcgagctgg
    14101 atgttctgca cgagctggca caggggctgt caaataaaca gattgcctcg gtgttgaata
    14161 tttccgagca gacagtaaaa gtacacattc gcaatctgct gcgtaaactc aatgtccgct
    14221 cacgcgtggc ggccaccatt ctgttcctgc aacaacgcgg ggcacaataa aaacagcccg
    14281 atggcatcac catcgggcct ctttttattt actctcctgc ggcgacaaat gttgcatcgc
    14341 ctgcgcgata ctacgttcaa tcaccgcacg gcgagtatcg ttggcaggta agagtttcaa
    14401 catcatctcc cacgcggcaa cggcttcgcc aaatcgctgc tgctcaaagg cgttaaacgc
    14461 atacatgctt aacacacgaa tattgctatg gtccgttctc accaactggc gcagcagttc
    14521 gccgccgagg cggttgtcgt tgggatcaga tgaacgtgtc aacgcttcag cgtatcccag
    14581 tgcagcatcg ctgttcttcg gatcgaggcg atacgcagta gcgtatgcat cggtggcgat
    14641 actggcgtta cccagcgcca ggccaacgcg gcccaacata atccagcctt ctatatctcc
    14701 cggatttttt tgcagttgag tacgcatccc cagcgccagg cgcgacatct cttcttcgtt
    14761 gagcggatcg gctttcggat ccagcgccct gtccagtaac gccggagcct gtgccgtggc
    14821 ctgctgccag attttcacct gctgatagtt gccagtctgg tagtagctga cgccagccac
    14881 aattaacgcc acaacaatac ccggcaggta aacagcaaat ccggcacgct taccttccgg
    14941 cacgctttgt tcaggaaacg cttccggcac cacgcgtacc cgacgccgcg aacgggcgta
    15001 aatgacccaa ccgccaatgc caatagccac taccggaagc acccacagca gcacggtcag
    15061 cggcgttaac ggcggatcgt aagtgacgaa gttgccgtaa cgcgccacca tataatcgac
    15121 aatctctttc ttacttttac cttcctgcat cagttcatac actttctggc gcaggtcggt
    15181 ggcaatcatc gagttggaat cggcaatgct gttgttctgg catttcgggc agcgtagttc
    15241 ttcagtgagt tgccggaact gctgctcctg cgcttcatcc ttaaactgca acacgtcgat
    15301 ggtcgccagc gctgagccgg agatcatcag catcagcacg cccaataaaa acctcattgt
    15361 gcggcctcct tactgtattt ctcccacagc ggcttgatct cttcttccca gacgcgagga
    15421 ttgagatcgc ccgcatggcg atagcgaatg atgccgttgc cgtcgatgag gaacgtttct
    15481 ggcgcgccat agacaccgag atccagtcct aacatgccgt cgccatcaaa caggcttagt
    15541 gcgtaaggat tgcccagctc tttcagccag ctgattgcct tctggcgatc gtctttatag
    15601 ttcatgccga ccacgcggat gccctgcgca gaaagctgat tcagatattg atgttccgca
    15661 cggcaggtcg gacaccaggt cgcccagacg ttaagcagca ctggtttgcc ctgagtcagc
    15721 acatccgcct gataaaactg ccccggattg tccagtgatt cgagacgaaa cttcggcact
    15781 ggcttgccaa tgagcgccga ttccagattg gtcggatcat ccccttcggc attacgcgcc
    15841 agctgccaca gcagcgccgc ggcaatcgcc aggaagataa tcaacggaat taacaatact
    15901 ttgcgcttca tacagcctcc ggcgcagttt tctgcggatt cacgcgctta cgatagcgag
    15961 gatca
//
LOCUS       DACBVI010000102        15960 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.102, whole genome shotgun sequence.
ACCESSION   DACBVI010000102 DACBVI010000000
VERSION     DACBVI010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.102"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            95..523
                     /gene="uspC"
                     /locus_tag="IU325_15790"
     CDS             95..523
                     /gene="uspC"
                     /locus_tag="IU325_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HAP5810464.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(530..1954)
                     /gene="otsA"
                     /locus_tag="IU325_15795"
     CDS             complement(530..1954)
                     /gene="otsA"
                     /locus_tag="IU325_15795"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HAP5810465.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(1929..2729)
                     /gene="otsB"
                     /locus_tag="IU325_15800"
     CDS             complement(1929..2729)
                     /gene="otsB"
                     /locus_tag="IU325_15800"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HAP5810466.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(2896..3885)
                     /gene="araH"
                     /locus_tag="IU325_15805"
     CDS             complement(2896..3885)
                     /gene="araH"
                     /locus_tag="IU325_15805"
                     /EC_number="7.5.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter permease AraH"
                     /protein_id="HAP5810467.1"
                     /translation="MMSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVP
                     NFATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTES
                     LWIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIED
                     ESFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPV
                     VRTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGK
                     ISYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(3897..5411)
                     /locus_tag="IU325_15810"
     CDS             complement(3897..5411)
                     /locus_tag="IU325_15810"
                     /EC_number="7.5.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HAP5810468.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(5481..6470)
                     /locus_tag="IU325_15815"
     CDS             complement(5481..6470)
                     /locus_tag="IU325_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP5810469.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            7267..7770
                     /locus_tag="IU325_15820"
     CDS             7267..7770
                     /locus_tag="IU325_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HAP5810470.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLDSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDASTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(7850..8101)
                     /gene="yecJ"
                     /locus_tag="IU325_15825"
     CDS             complement(7850..8101)
                     /gene="yecJ"
                     /locus_tag="IU325_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HAP5810471.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8217..8303)
                     /gene="azuC"
                     /locus_tag="IU325_15830"
     CDS             complement(8217..8303)
                     /gene="azuC"
                     /locus_tag="IU325_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HAP5810472.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            8564..8887
                     /locus_tag="IU325_15835"
     CDS             8564..8887
                     /locus_tag="IU325_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810473.1"
                     /translation="MRLLILTLSLITLVGCTVTRQAHVGEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9058..9555
                     /gene="ftnA"
                     /locus_tag="IU325_15840"
     CDS             9058..9555
                     /gene="ftnA"
                     /locus_tag="IU325_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HAP5810474.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(9593..9832)
                     /locus_tag="IU325_15845"
     CDS             complement(9593..9832)
                     /locus_tag="IU325_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2492 family protein"
                     /protein_id="HAP5810475.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFTPSEDGFSTDQSKICRH"
     gene            10024..11235
                     /gene="tyrP"
                     /locus_tag="IU325_15850"
     CDS             10024..11235
                     /gene="tyrP"
                     /locus_tag="IU325_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HAP5810476.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRRHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11297..11962)
                     /gene="yecA"
                     /locus_tag="IU325_15855"
     CDS             complement(11297..11962)
                     /gene="yecA"
                     /locus_tag="IU325_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HAP5810477.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPSLEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12158..12244)
                     /locus_tag="IU325_15860"
     tRNA            complement(12158..12244)
                     /locus_tag="IU325_15860"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12208..12210),aa:Leu,seq:taa)
     gene            complement(12257..12330)
                     /locus_tag="IU325_15865"
     tRNA            complement(12257..12330)
                     /locus_tag="IU325_15865"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12296..12298),aa:Cys,seq:gca)
     gene            complement(12384..12459)
                     /locus_tag="IU325_15870"
     tRNA            complement(12384..12459)
                     /locus_tag="IU325_15870"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12424..12426),aa:Gly,seq:gcc)
     gene            complement(12611..13159)
                     /gene="pgsA"
                     /locus_tag="IU325_15875"
     CDS             complement(12611..13159)
                     /gene="pgsA"
                     /locus_tag="IU325_15875"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP5810478.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(13216..15048)
                     /gene="uvrC"
                     /locus_tag="IU325_15880"
     CDS             complement(13216..15048)
                     /gene="uvrC"
                     /locus_tag="IU325_15880"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP5810479.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(15045..15701)
                     /gene="uvrY"
                     /locus_tag="IU325_15885"
     CDS             complement(15045..15701)
                     /gene="uvrY"
                     /locus_tag="IU325_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HAP5810480.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
BASE COUNT         3995 a         4086 c         3712 g         4167 t
ORIGIN      
        1 tatttacctg tttgaccgag ttgggattgc gtcgtttctc cattaggagt aaagctttaa
       61 tgtcacctga agttcacaga ataaggaaca ggttatgagc tatagcaata ttcttgtcgc
      121 tgttgcggtt acaccggaaa gtcagcaact gctggcaaaa gcggtatcta tcgccaggcc
      181 agtaaaggga cacatcagtt taattactct cgcttccgac ccggaaatgt acaatcaatt
      241 agctgcgccg atgcttgaag atttacgcag cgtgatgcag gaagaaacgc aaagctttct
      301 tgataagtta attcaggatg cggggtatcc cgttgacaag acgtttattg cctacggtga
      361 gttaagcgaa catattctgg aagtatgtca caagcatcat ttcgatttgg tgatttgcgg
      421 taatcacaat cacagtttct tttcgcgagc atcctgctcg gcgaaaagag ttattgcctc
      481 aagtgaggtc gatgtgctgt tagttccact tacgggagat taaccgctcc tacgcaagct
      541 ttggaaaggt agcaacttta tcgcgctgct ggctttccgc gcttcgcgga actatctgct
      601 ttaggtcgct aatgaagcac tcctgccagt ggttaatatc gtttttcacg ataacgtcca
      661 gcatttctgc atgacgggaa atacgttccg ccagcgacat agtcaatgca cgatccagcg
      721 cagctgcaac ttcgtcacga tcgtaggggt taacaattaa cgccgacgtt aactcgtttg
      781 ccgctcccgc aaattgcgaa agaacaagaa cgcccggatt ggctgggtcc tgagcagcaa
      841 catactcttt tgctaccagg ttcatcccgt cacgcagtgg cgtcactaag cccacgtcag
      901 agtagcggaa tattttcatc agtaatttac ggtcaaaatg ctgattcaaa taataaagcg
      961 gcgtccagcc taattgcccg tatttaccat taattcgtcc agcttcattt tcgagctgat
     1021 gacgaatatc ctgataggct tgcacatcac cacgcgacgt tggtgcaatc tgggtataac
     1081 gaattttacc atgatgctgc ggatattttt ccagcaacgc ttcataggcg agaaaacgct
     1141 ctggcaaacc tttggaataa tccagccgtt cgacagaaaa gatattttgt acgtttttca
     1201 gttccgcttt aagttgcgcc agttttggcg gcagtggccc ggcagcctgt ttggctattt
     1261 ctttcggttc aatgccgatc gggtagactt ctgttcgaaa tgctttgccc caggctgtat
     1321 ggctttttgc gctacgtgtc gtgacgcggg tcaggttaga aagacaatcc aggaacgcca
     1381 gacgatcgtt ttctgtctgg aaacccagca aatcataatc acaaagctgt tcaagcaagg
     1441 tgtcatatgt cggcagcgcg ttgaagattt ccggtgtcgg gaaaggaata tgcagaaaga
     1501 aaccaatgcg attattcact ccccgtttgc gtaattcatg cgcaaatggc aacaggtgat
     1561 aatcgtggat ccagataatg tcatcgtctt gcaacagcgg cagtaattta tctgccagca
     1621 acgcatttac gcgtagatag ccgtcccagg caggacgctg aaattgcacc agatcgagcc
     1681 gataatgaaa agcgggccag agaacggcat tggagaattg gttgtagtat tcgtcaaggt
     1741 cctgttcgct gaggttaaaa gaggcccacg taatgttacc ttttttcacc ttttttagcg
     1801 gctgatcctc attccctgtt tcaccactcc agccaaacca cagtccgcct gcggctttca
     1861 gtgcccccag tatgccaacg gcaaggccac cggcactggc ggcgtgctcg tctggtggtg
     1921 caatccggtt agatactacg actaaacgac tcatagtcat cactcctgtt attttctctt
     1981 ttttgttgta atgcggtggt tatcatttca agccagctcc agacatccgg cacacccgcc
     2041 agtcgccatg atgcctgagt tgcacctgtg ccaattttta ctgacattcc gcccagtcgg
     2101 ttaacgactg cgaagccaga ttcatcggtt aaatcatcgc ccagaaatac gggcgttcgc
     2161 ccgataaagg gagcttcctg cataaaagct gcaattgcct cacctttact ggtacctctc
     2221 ggtttgatct cgacaacaca ctttccctgc tgtaacgcca tttgtggcca gatctgagta
     2281 atacgttgcg ctaatgtcat taatgcgtct tcatgctgcg gagcctgacg ataatgcagc
     2341 gcaaaagcca tccctttcgc ctccagctcc gcgccgggat actgagcgat gactgtatgc
     2401 agttgcacgc taatatcacg cgcaatcgca tccggcagat gaacgatatg tgttttacca
     2461 ttgatgtcac ggcgctccgc cccatgcacg cccgctaacg ggaagcgata aggttttgcc
     2521 agtgcgtcaa gctccaccat tgagcgccct gatatcaatg ccaatgcacc atcacttgcg
     2581 gttgccagta gctgtagtcc ttgcagaata ttgtcaggca cgacgacctg atcgggatgc
     2641 ggtttgattt ccgccagcgt tccatcaaga tcaaaaaacc aggcatattt cgcggatagt
     2701 tcaggggttt cggttaacgg ttctgtcacc cggttctcct ccttctcttt tcttattgtg
     2761 ttgcgcagaa cgcgctttac agacatgtaa gtatagacag tgtgacgggg gtcgccattt
     2821 gcaacgaaga acagccaacc gttatggcgg ttggctatgg tgggaaaaaa cgctaaattg
     2881 ttgcagaaaa aagcatcaga cagtgcgttt cgctttttgc ttgtaacggt cgaagatcac
     2941 cgctgccagc aggattaagc cgcgaaccac gtactgcgcg aaaggagaaa tattaagcag
     3001 gttcatggcg ttttccacgg tgcctaaaat taagataccc gccaccacat atgagatttt
     3061 tccgatgcca cctttcagag aaacgccacc taaaacgcag gcggagataa caatcagctc
     3121 ataaccaatc gacgtcattg gctgcccact ggtcatacgt gaagccagaa taattccggc
     3181 tatcgctgat accaggcctg agagaacaaa gataataatt ttggtgcgaa caaccggtac
     3241 acccgccaga cgcgcggcct cttcgttccc gccaatcgcc agggtgttac gaccaaaggt
     3301 ggttttattc agcagcaaac caaagataat cagacacgcg acggtgagcc agattggcgc
     3361 aggcagaccg aaccagttgg cgtaaccaag ggcaaagaag ctttcatctt cgataccgac
     3421 cgctttaccg tctgaaatga tgtacgccag acctcgaaca atctgcatcg ttgccaatgt
     3481 cgtgatcaga gcatttattt tcagtttggc gataacaaag ccattgacca ggccacagag
     3541 aacgcccagc aacaaccccg ctgccacgcc aatccacagg ctttcagtca ggttaataac
     3601 caccgccgtg gtgacacccg cacaggcaat tacggaggcg acagaaaggt caaagtcacc
     3661 ggaagcgagg cagaacaaca tgccacaagc caccatcccc gacatggaaa ttgccaggcc
     3721 caaccctttc atattaatga aggtggcaaa atttgggaca aaaatggcac aggcgataaa
     3781 gagcaccgca aacaccacca gcatgccgta ctgatcccag atacgcccga agctgaatga
     3841 cgacttaggt gcgccagacc ccgatgtaga aacagaagac atcatactct ccttactcag
     3901 gcaacagcct ggctgacttt aggcatcgca aggctcagtg cctgacgctc atctgcctgc
     3961 tcgtgtaaca attcaccggc gatttcacct tcccgcatca ccacaatccg gtcggcaacg
     4021 ccgaggactt caggtaagtc gctggaggca aacagcaccg ccacgccctg cgccgccagc
     4081 gcataaatta cgttatatat ttcgtgctta gcgccaacat caatgccgcg cgtaggttca
     4141 tccagcaaaa tgaccttcat ctcttccgat aaccagcggc ccagaatggc tttttgctga
     4201 tttccgcctg agagattcat gatcagttgc tccgcgcccg gcgttttgat gttgagcgaa
     4261 cgaatgtggt gatcggcatt gttttcttcc caaccgttgt tgattacaca accgccgagc
     4321 acatgtttac gtctggcact gatgttgata ttgtcgcgaa cggagtgcac gggaataatg
     4381 ccttccgctt tgcgatcttc cgggcagagc atcatgcctg cggcaatggc gtggctcggt
     4441 ttacgaatat cgatcggctg ttggtcgata taaacctgac cggcggtgat ttgcgtcccg
     4501 ccaaacatgc ctttcattaa ttcgctacgc cccgccccta ccagaccaaa cagcccaaca
     4561 atttcaccac tgcgaaccgc cagacttatt ggcgtacgca cgcctggtgc tttcacagca
     4621 tcaagacgca ggcgctcctc gccataacta cgcggttgcc agccgtagat atcgccaatg
     4681 tcgcgcccga ccatcgcctg caccagcgcg tcgtggtcaa cctgctgcat atcggtaaag
     4741 gttttgacat aacgtccatc tttaaagaca gtaatggcat cgctgagggc aaatatttct
     4801 tccatacggt gagaaacgta taagattacc cgcccctctt ttcgcagttc acgaataacg
     4861 cggaaaagat tgtcgatttc acgggcagag agggagctgg ttggctcatc aaaggcgata
     4921 attttggcgt tacgcgccag cgctttggcg atttcaacca tctgccactg accaatggag
     4981 agatatttca gcggcgtgtc cgggtcaata tccataccaa gatgtttaag ttgtaaaccc
     5041 gcctcataat tcagcaatga gcgattcaca atgccgcctt tatgcggcag ctggccgaga
     5101 tagatgtttt ccgcgacggt catttccggc acgagatgca gttcctggta aataatcgcc
     5161 acgcccgcgt taagtgctgc ggtcgtgtcg gaaaaggaca tttcctgccc attaatcact
     5221 acagaacccg tggttggcgc atagttgccg ctgaggattt ttaagagagt tgattttcct
     5281 gcgccatttt cacccatcaa cgcatgaacc tgaccggcat agcagtcaaa actaatatcc
     5341 gtcagcgcct taacgccggg aaacgtttta ccgatgccgc gaaatgagag atacggggta
     5401 gactgttgca taacgtctcc gtgaatcact ggtcgttcac accccctctg catcatgcag
     5461 aggggaattt ttccggcaaa ttacttaccg cctaaacctt ttttctccag ttcttcttta
     5521 aagttgtcac gcgtgatcag taccacgtcg gtaacttcgg taaattttgg cggttcaacg
     5581 tcttttgcta cccagttgta aagcatttcg ctggatttat agccatgtac gtccgggctt
     5641 ggcagcaggg aaccgtagaa gccggttgcc tgtgctttag acagttcgct caccgcatcc
     5701 acaccgttaa tgccaatgcc gatgatatcg gccgctttaa agccctgacc ttccgtcgcg
     5761 cgtacgccgc ccagcacggt gctgtcgttc ataccgacga tcagccaatg tttaacttcc
     5821 ggatgttgaa ccagcattga gttggcagcg tcaaatgccc ccgggatgtc gttagatttg
     5881 gtaggtacct gataaatttg tttttccggg aatccggccg ctttcagcgc atccatagat
     5941 cccgtagtac ggcggcgggc ggtatccagt tcgttggcgg taatcgccat caccgcgctt
     6001 tctttgacat cccagccacg tttctgcatc tctttataca gttcctggcc ctgacgttcg
     6061 ccaattttag tcgccgccat catcaccagc ggaacggtat ccattggctt acctttggcg
     6121 ttaacaaact ggtcatccac ggcaatgact ttcatatcgt agccacgcgc tttcgccacg
     6181 atggcagagc cgagtttcgg gtccggtgta caaataacga aaccttttgc gccactggcg
     6241 gccaggctat cgatcgcgtt caatgttttt tcgccatccg gcacggcaat cttaataacc
     6301 tcaaacccta aatccttccc ggctttatcg gcaaacttcc attcggtctg gaaccatggc
     6361 tcttccggtt gcttcaccag aaaaccgagc ttcaggttct ccgccatagc ggattgtgac
     6421 ataacggctg ccagaccaat ggctgccagg gctttagtaa atttgtgcat ggttctctcc
     6481 agctttagtg tcgttttgtg tagggcaaaa acgaatgaca ttcgttaaat taatcggaaa
     6541 acaaagcatt accttttaac taaaagataa gtgactgtgt tgacatagtt ttagcgagaa
     6601 attaattctc cataggagag caatatcaca tcgcagaatt acagtgagaa cgtgcataaa
     6661 tttagcggga aaagacataa gggaaagcca atttgtcaga caaattgtcg aatgcacagc
     6721 agattaatcc ataagattag cctggaaatc cttgttgtct ttggtaccca tgcgggatgt
     6781 cttcttttta accagtcaat aggccgcatt acctggcgtt gagtttttga aatggtgtaa
     6841 taaccacaac tcaaagatgt ggaaaatgcg cgtcattcat ttcgccatta attatcactg
     6901 tgctcattaa ttaacagaac acgtataatg agagccatct cgcaaaaatg aaaaaacgtt
     6961 ttataaaatc atcacttcat catgaattca aattcattga ttaatatcaa caagatacaa
     7021 aaagcactat cattaaaatt cattgcagtt acattgattt catcaatgaa atgtaaaaat
     7081 atataaactt gatgatttaa gcattttctt atacccgttc agacgttatt cttatttcag
     7141 atcatcgtca gaattgactc cacgatcaca tttcggaccg gcagaaagga attattctgc
     7201 aaacagtaat tatggtgttt tgatttatct tgcacctctc cacttctgga tataaggata
     7261 ttaggtatgg caaccgctgg aatgcttctc aaactcaact ctcaaatgaa ccgcgagttt
     7321 tacgcatcca atctctacct tcacctgagt aactggtgtt ctgaacagag tctgaacggc
     7381 accgccactt tccttcgcgc ccaggcacag agtaatgtga cccaaatgat gcgcatgttt
     7441 aactttatga agagtgtcgg cgctaccccc atcgttaaag ccattgatgt tcccggtgaa
     7501 aaactggact ctctggaaga actgttccaa aaaacgatgg aagaatacga gcaacgttca
     7561 agcacgctgg cgcagttagc cgatgaagcg aaagaactga atgatgcttc aaccgtcaat
     7621 ttcctgcgcg atctggaaaa agaacagcag catgatggcc tgttgctgca aaccattctt
     7681 gatgaagtgc gcagtgcgaa acttgcgggt atgtgccctg tgcagaccga ccaacatgtt
     7741 ctgaatgtcg tgtcacacca gctgcattga tcatacatcg gcgctaatgc attgcgccga
     7801 tgaagttttt gagaaaccgc tgcctcatct gtttgaagca gcggtttttt taatgggatt
     7861 caccctgtgg ggtaaacttg agctcaatca gcgcgatggc tttttggatt gcccgcatgg
     7921 tgaccgggtc tgcggcagcg ggatgatgag taaagtcgat attcttcagc tgactggaca
     7981 ttttttcacg aacttcaacg ggcgcgatta catcgagaac atccagaatt tgtttgataa
     8041 ccaactggca agcaaccaca tcagaaacca gttcctgatc ggcattcagc ggctgggaca
     8101 tcgtaaactc ctgatagcat tttgaaagcc gttatagtag cggcttcaca tcttcagcga
     8161 tagtcacatc caccgtcatc aggacacaaa aaaacctgcc ggagcaggtt tttttgttat
     8221 cggaacatat tgcctggcgg tacgtctttg aacgtcttgc aatagttatt gaacatactt
     8281 ttcaggattt tgcgcagttt catcgcggca ctccgactat ttgttatgca ggtgttattg
     8341 tctttgcact tataatatga caaccatcac aaaaatcaat ctttatgtga tgcaaatcac
     8401 ataaataccc ctttaatgtt ataaaaatga taatcaaaaa gcgccccccc tttctgacac
     8461 ctacagatgg cgagaaatag cgcctgccag gcgtcttttc cggccattgt cgcagcgctg
     8521 taacgcgtaa aatagtgctt tcttttactc ttctggctgg accatgagac ttctgattct
     8581 gactctttca ttaataacgc tggtggggtg cacagttact cgccaggctc atgtgggcga
     8641 agttgacgcg gcaacaggta ttgttcggtt ggtttatgat caggcttttt tgcagcatgc
     8701 ccataccgat cgttatgtca gtcgcgggat cgccgatcgc gcatgccagc aggaaggcta
     8761 tacccacgcg atcccctttg gtcagccagt aggcaactgt agcctttttg cgggttcctt
     8821 atgtttgaat acggaattca ctttgtcata tcagtgccat cattccgcct tccctgtctt
     8881 tctctaaata ttttaatcag cgaggggatc ttcgctgatt aaagaaatag cggtaatgcg
     8941 ttttattccc attcgtattt ttaataattg aagtttatat tttacctttt gcaaataata
     9001 aaataacaaa ttatagtgac gccacagcaa caaatataac ctttgtggag cactatcatg
     9061 ctgaaaccag aaatgattga aaaacttaat gagcagatga acctggaact gtactcttca
     9121 ctgctttatc agcaaatgag cgcctggtgc agctatcata ccttcgaagg tgctgccgcg
     9181 ttcctgcgcc gtcacgccca ggaagagatg acgcatatgc agcgtctgtt tgattacctg
     9241 actgataccg gcaatttacc gcgtattaat accgttgaat ctccgtttgc tgaatattcc
     9301 tcacttgatg aattattcca ggaaacctat aaacacgaac aattaatcac ccagaaaatt
     9361 aacgaactgg ctcatgctgc aatgaccaat caggactacc caacatttaa tttcctgcaa
     9421 tggtatgttt ctgagcagca tgaagaagag aaactgttca aatcgattat tgataaatta
     9481 agcctggcag gcaaaagcgg cgaaggtctg tattttatcg acaaagaact ctctaccctc
     9541 gacacacaaa actaatgctc tcaggcggca ggttttctgc cgccgtttca aatcagtgac
     9601 ggcaaatctt actctgatcg gtcgaaaaac cgtcttcaga aggagtaaat ttgccttttg
     9661 ctgccagaaa cgccaccagc tctcccgctg tcatcccttc tgccgagcaa gtgtgaaaac
     9721 gtgcctgttc accaaaacgc gctttaatcg cagcttccag actggcatgc gtatattgct
     9781 cgcctgattc aatcatcata tttaacactt catgaccgtg aatagagtcc atcgttcctc
     9841 ctcaaaaaaa agcctagcgt agcgattgcc gcttatgaag actttgcgcc agcgcaggac
     9901 tgaatgcttt ttattgtaca tttatattta caccatatgt aacgtcggtt tgacgaagca
     9961 gccgttatgc cttaacctgc gccgcagata tcactcataa agatcgtcag gacagaagaa
    10021 agcgtgaaaa acagaaccct gggaagtgtt tttatcgtgg cgggaaccac aattggcgca
    10081 ggcatgctgg caatgccgct ggctgcggcc ggtgttggtt ttagcgttac gttaatcttg
    10141 ttgattgggc tttgggcgtt gatgtgctac acggcgctat tactgctgga ggtgtaccag
    10201 catgttccgg cagataccgg tctgggcacg ctggcaaaac gctatctggg acgctacggt
    10261 caatggctga cgggcttcag tatgatgttc ttaatgtatg ctctgactgc ggcatacatc
    10321 agcggtgccg gtgaattgtt ggcctccagc atcagcgact ggacaggtat ttcaatgtcg
    10381 gcaaccgctg gcgtgctgtt gttcactttt gttgccggtg gcgtggtttg tgtcgggaca
    10441 tcgctggtcg atttatttaa ccgttttctg ttcagcgcca aaattatttt tctggtggtg
    10501 atgctggtat tgctgctacc gcatattcac aaagtgaatc ttttaaccct gccgttgcaa
    10561 caggggctgg cactgtctgc aatcccggtg atttttacgt cgtttggttt tcacggtagc
    10621 gtgccgagta ttgtcagcta tatggatggc aacattcgta agctacgctg ggtgtttata
    10681 accggtagtg cgatccccct ggtggcatat attttctggc aggtggcgac gcttggcagc
    10741 attgattcaa caacctttat gggattgctg gctaatcatg ccggattaaa cgggctgtta
    10801 caggcgttac gcgaaatggt ggcctctccg catgttgagc tggcagtgca tttatttgct
    10861 gatttagccc tcgccacgtc atttctcggc gttgcgttag gcttatttga ttatctggct
    10921 gatttgtttc agcgttcaaa taccgttggt ggacgattgc aaactggagc aattaccttt
    10981 ctgccgccgt tggcgtttgc actgttttat ccacgaggat ttgtgatggc gctgggttac
    11041 gccggtgtgg cgctggcggt actggcattg attatccctt ctctgttgac ctggcaaagc
    11101 agaaggcaca atcctcaagc gggttaccgg gtcaaaggtg gtcgtccggc gctggtggtg
    11161 gtgtttctct gtggtattgc tgtgattggc gtgcaatttt tgattgcggc agggttgtta
    11221 ccagaagtgg ggtgatcaga tagcctcaaa ttccttattg ggtgccagaa ttaacgctga
    11281 cacccaattt gacttcttaa tgcaggcagc actgcttaaa tttcttacca ctaccgcacg
    11341 ggcaaggatc gttacgcccc ggtttctctt ctgctttgat cggttgctga acagcttttt
    11401 cctgcggatg cgccatccag tacgcatgta gatcaagcgc cgccagtcga atggcatcta
    11461 cgctctcttc aaacgcttct ggcgacatct tctctacccg ctcgaagttt tcctcagtac
    11521 cgtgcagcgc aatcgcctcc agcgatggtt ttaacgaatc gggcaatgtt gaccagtcag
    11581 aaagtgccac gccccgcata tagccaaagc accactcctc aacaatcgtc agctcgctgc
    11641 catcaacttc tcgcaagccg aataacggct caaactgctc cgggaattcg ttcagacgct
    11701 ctgcggtatc ggccatatgt tgaaaagcca ggttcataaa gcgcgtcatc tctttctctg
    11761 acgcccagcg cggcacatag tcagccccac cccacacggc aaccagccac tgtgccggtt
    11821 caatctcttg cggagaactc aacaccgccg tcaataaacc gtccagctcc gccacatcaa
    11881 ggatggcgtg gtcagtgttg tatttggtca gaatatcgtc cagccattcc aactcacttt
    11941 cgtttaacgg tcccgttttc atacgctttt ccttgtggat ctcaactcgc cagcacctat
    12001 cttacatgcc ggtccgtatc agagatactt tttgagtggc tttgccggtg attaaaaatt
    12061 aaggagggtg taacgacaag ttgcaggcac aaaaaaacca cccgaaggtg gtttcacgac
    12121 actgcttatt gctttgattt tattcttatc tttcccatgg tacccggagc gggacttgaa
    12181 cccgcacagc gcgaacgccg agggatttta aatcccttgt gtctaccgat tccaccatcc
    12241 gggctcggga agaaagtgga ggcgcgttcc ggagtcgaac cggactagac ggatttgcaa
    12301 tccgctacat aaccgctttg ttaacgcgcc aaattcttca ggcctttcag ccagacatcc
    12361 gctgacgccg atgtctttta aactggagcg ggaaacgaga ctcgaactcg cgaccccgac
    12421 cttggcaagg tcgtgctcta ccaactgagc tattcccgca ttcatcaagc aatcagttaa
    12481 tcacttgatt ttattatcgt ctggcaatca gtgccgccgt tcgatgcgtt gcattctact
    12541 tacctggcgc gatgagtcaa cgatattttt caccactttt gatcgtttgc tgaaaattgc
    12601 gccgaaacga tcactgatca agcaaatctg cacgcgcagc gctcaaatat tgcaacattg
    12661 accacagagt cagtaccgca gccacaaaga aaagtgcaat accggcgtac tcaacccaaa
    12721 tgttcggacg ccacagcagc catgccaacg ccaccatctg ggcagtggtt ttcactttcc
    12781 caatccagga gacagccacg ctactgcgtt tacccaactc cgccatccat tcgcgtagcg
    12841 cagaaataat aatttcacgg gcgatcatcg ttgccgccgg taaggtcacc caccagctgt
    12901 ggtaatgctc ggttaccagc accatggcga tagccacgag aactttatct gctacagggt
    12961 caaggaaagc accaaaccgg gtactctggt tccagcggcg tgccagaaaa ccatcgaacc
    13021 agtcagtcac cgccgcgacg cagaaaatga gcgcggcggc aaacggcgac caggtgacag
    13081 gcagataaaa gaccaataca aagaatggga taaggatgac acggaacagt gtaagcaacg
    13141 tagggatatt aaattgcata atgacgggta actatctgtt gtcagtaaga ttacccctat
    13201 gttgctacag agacatcaat gtttcaacga ccagaagatc ttttctgcca gaccttgcga
    13261 aatacccggc acttttgcaa tttcctcaac gctggcgtta cgtaaacctt gcaaaccgcc
    13321 catatatttc aacaacattt gccgacgttt tggcccgacg ccttcaatgg tttccaggga
    13381 actggtattt ttgaccttcg cccgtttttt acggtgcccg ccaatcgcgt gatcatgtga
    13441 ttcatcgcga atatgctgga taacatgcag cgcgggagaa tctggcggca aactgaatcc
    13501 ctcaccttcc ggctcaaaga acagcgtttc cagcccagcc ttacgatctg ctcctttggc
    13561 aacgccaagt agcagcggat gatttttatc ccatgagaca tccagttcgg cgaagacatt
    13621 tttcgcctgc gcaagctggc ctttgccgcc gtcgataagg atcacatccg ggatcttact
    13681 gtcgtcaatg gctttaccat aacgccgacg cagcacctga ttcatcgccg cataatcatc
    13741 gcccggtgtg atgccagtaa tgttatagcg ccgatactcc gcacgcagcg ggccgttagc
    13801 atcaaacacc acacaggaag cgacggtttg ttcacccatg gtatggctga tgtcaaagca
    13861 ctccatccgc ttcacttccg gcaatttcaa cacgctggca agcgcggtca gccgctggtg
    13921 aacggtagat tgctgcgaaa gtttgctggt taaggccgtc gccgcattgg tgcgcgcgag
    13981 tttcagataa cgcgccctat cgccacgagg tttggtttga acattaatct tgcgtcccgc
    14041 cagttctgaa agggaatcgg cgagcagcgt tttatcgcta agattaaaat cgagcaggat
    14101 ctcacccggt aaggtgcgca tctggctgcc ttgtaaatag aactgaccta cgaaggtttc
    14161 caccacctcg ctcagttccg taccgccagg cactttcggg aaatagctgc ggctgccgag
    14221 cactttaccc tgacgaatga acaatacgtg gacacaagcc atgcccgcat cgaacgccac
    14281 accaataacg tcaaggtcgt cgccggtatt ggaaacaaac tgtttttcgg tgacgcgtcg
    14341 caccgcctga atttggtcgc gaatacgtgc cgcttcttca aactccagat tctgactggc
    14401 agtttccata cgactaatga gttgcgtaag cacctgatca tctttgccag acaaaaacag
    14461 gcgcacatac tcgacctgct gagcgtattc ttcttcactc accagtcctt caacgcacgg
    14521 ccccagacag cgccctatct gatattgcag acatggacgc gagcgattgc gataaacact
    14581 attttcgcac tggcgaatgg ggaaaatctt ttgcagtagc gccagtgttt cacgtacggc
    14641 atagccattc gggaacgggc cgaaatattc acctttggca tgctttgcgc cacgatgcat
    14701 cgccagacgc gggtgggtat caccactcag gaagataaaa ggatatgatt tatcatcgcg
    14761 tagcaaaacg ttgtaacgcg gctgatagag tttgatgtag ttgtgttcca gcaacagcgc
    14821 ttcggtttct gtgtgagtaa ccgttacatc aatttgctgg atctgggcga ccagcgcttc
    14881 ggttttgcgc gaagcgaggt tgctacggaa atagctggaa agccgttttt tcaggtcttt
    14941 cgctttgccg acatagataa ccgtaccacc agcatcgtac atgcgataaa cgcctggctg
    15001 gctggttacg gtttttaaaa acgcttttgc gtcaaactga tcactcactg acttgataat
    15061 gtctccgcat tacacagacc atggcgaatt gccaggtgag tcagctcaac atcgccatga
    15121 atgtttagtt tactgaacat acgatagcgg tagctgttca ccgttttcgg actgagattg
    15181 agctgttctg agatctcatt gaccttctgg cccttggtga tcatcagcat aatctgcaat
    15241 tcacgttcag acaaactggc aaatgggctt tctgtttttt ctggttcgat ctggcttaac
    15301 gccatttgtt gagcgatgtc agaagcaatg taacgctgcc ctgaatagac agaacgaatc
    15361 gcactcacga cttcctgcgg agccgcgcct ttgctgaggt agcccgcagc accggcctgc
    15421 atgactttcg ctggtaaagg gttttctgta tggacggtaa gcatgataat ttttacatca
    15481 gctgtggaac gcgcgatttt acgcgtcgcc tcaagaccgc caatgcccgg catactcatg
    15541 tccattagca ccacgtcaac ggcatttgtc cggcaccact taacggcgtc ttcaccgcac
    15601 gatgcctcac cgacgacttt tatacccttt atatcttcca gaatgcgtcg tatccctgcg
    15661 cgcaccagtt cgtggtcatc aacaagtaga acgttgatca aaggaatatc tccagaaata
    15721 gggataacgc tactgatagt tagtcattcg tatattagcg gtttttattg caactttgaa
    15781 acgttaaaaa tggtgggtat tcgatttttc tctcgttttt gtcaattcga ttgtccacag
    15841 ttatggtcac gcccgtcacc aacggcttcc gggatcgttt cacagtaagc actgcgacga
    15901 aatgtgacaa aaattgactt tattcagcaa aaatgaaaat cagcccgctg aatatgtaac
//
LOCUS       DACBVI010000103        15943 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.103, whole genome shotgun sequence.
ACCESSION   DACBVI010000103 DACBVI010000000
VERSION     DACBVI010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.103"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            103..1437
                     /locus_tag="IU325_15890"
     CDS             103..1437
                     /locus_tag="IU325_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="involved in the transport or adenine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP5810481.1"
                     /translation="MNNDNTDYLSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGQFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1490..1942
                     /locus_tag="IU325_15895"
     CDS             1490..1942
                     /locus_tag="IU325_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 family protein"
                     /protein_id="HAP5810482.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2058..2381)
                     /locus_tag="IU325_15900"
     CDS             complement(2058..2381)
                     /locus_tag="IU325_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP5810483.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSSVDAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LEVLL"
     gene            complement(2365..2718)
                     /locus_tag="IU325_15905"
     CDS             complement(2365..2718)
                     /locus_tag="IU325_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP5810484.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADALGGGVFKKR
                     LHQNRERAIILAKGGINWFYTFLYAKQDMSNINSQELAGFRELAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            2951..4135
                     /gene="nepI"
                     /locus_tag="IU325_15910"
     CDS             2951..4135
                     /gene="nepI"
                     /locus_tag="IU325_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HAP5810485.1"
                     /translation="MSEFIAENRGADVITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MK"
     gene            4336..15465
                     /locus_tag="IU325_15915"
     CDS             4336..15465
                     /locus_tag="IU325_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810486.1"
                     /translation="MAGKAHGNGDRRGDNTICGLGDRLRRLTAGICLITQTIFPVMAA
                     APTHINPAHSDTAASLILPNVKTIPYTLGALESPPTVAARFGITVDELRRLNQFRTFA
                     RGFDNVRQGDEIDVPLINSNSPEARNLKAMQMERDGKDPQMQVAEMAQQSGTLLARDM
                     DSEQAASMARGWVASSASAQATDWLSRWGTARVSLGVDEDFSLKSSSFEFLHPWYETP
                     DNLVFSQHTLHRTDNRTQTNHGIGWRYFTSSWMSGVNMFIDHDLTRYHTRTGMGVEYW
                     RDYLKLSGNGYLRLSNWRSAPELDNDYEARPANGWDLRAEGWLPAWPQLGGKVVYEQY
                     YGDEVALFGKDERQNDPHAITAGLSYTPVPLISFSAEQRQGKQGENDTRIGMELTLQP
                     GHSLQKQLDPAEVAARRSLVGSRYDLVDRNNNIVLEYRKKELVRLTLTDPLKGKPGEV
                     KSLVSSLQTKYALKGYDIEAASLQSAGGKVAVSGKDIQVTIPPYRFTAMPETDNTYPI
                     AVTAEDSKGNFSRREESMVVVEKPTLSLAGSTLSVDLQILLADGKSTSTLTYTARDSS
                     GKPIPGMTLKTQVKGLQDFALSEWKDNGNGTYTQIVTAGKTSGALSLMPQFNGDDIAK
                     TPALIAIVANTASRADSTIETDQDNYVAGKPIVVKVTLRDDNGNGVTGRKELLKQTVK
                     VDNTKADAVSAWTEESEGIYKASYTAHLIGDKLTAQLTMPGWQTKHSDAFSIAGDKDT
                     AKIAAMQITANNAVARRDHNTVAVTVRDVHQNLLQGQNVTFTVVNGAAVFADPNGGIV
                     TTDKDGIASVNLASDQAVNSLIKAETNGSSQSVEVSFITGDISQLTSTIKTDDVSYTA
                     GGKIKVSVTLMDEQKNLVKGMASLLAGSGVVEVSGTDKNETGNWSEESDGVYTTTRTA
                     KIAGDRHYATLKLSTWSSAQQSDAYAIRESGAVLAYSSIVTDKTTYTAGGAIKVTVTL
                     KDSYENLVGGQRDAINLAIQLPNTKAESIAWNEDQKGIYTATYTALLPGTGLKAQLQM
                     SGWANALTSNDYSISGDAASAQIVAMQVTTGNPDVLANGSDRHTVNVRVEDQFGNVLP
                     EQTVTFTVTKGAAVFANAGQSAGIRTDAHGMAEVDLSSTVADASTVEAKINQSSDSKT
                     VNFVADVSTAQVAELVVIKDGSEADGSTANTLRVKVTDAFGNTLAGQTVSVLADNGAT
                     VAPTVITEPDGTVEISVTSQTAGTSVVTASVNNSSQSRNVTFVADVRTAKIADLVVTR
                     DNSVADGAMANTLRVRVTDAFGNTLAGQTVSVMADNSATVSPTVTTEPDGTVEISITS
                     QTAGTSTGTASINNSSLSRNVTFIADVRTAKIADLVVIKDDSVADGVMANMLRARVTD
                     AFGNVLAGQTVSVTADNGATVAPVVITGPDGTVEISVTSQTAGTSAITASINNSSLSR
                     NVTFVADVRTAKIADLVVTRDNSVADGAMANTLRVRVTDAFGNTLNGQTVSVLADNGA
                     TTAPTVTTQPDGTVEISVTSQTAGVSTVTASINSSSLIRNVTFVADVRTAQIASLEVT
                     RDNSVADGAMANTLRVKVTDAFGNALNGQTVSVMADNGATVAPTVITEPDGTVEISVT
                     SQTAGVSAVTATINSSSQSQNVTFIADVSTAKIADLVVIKDGSEADGSTANTLQVKVT
                     DAFGNALAGQTVSVMAGNGATTAPTVTTQPDGTVEISVTSQTAGASTVTASINNSSLS
                     QNVTFVADVSTAKIADLVVIKDGSEADGSTANTLRARVTDAFGNALAGQTVSVMAGNG
                     ATVAPTVITEPDGTVEISVTSQTAGISAVTASINSSSQSRDVTFIADVRTAKIAELEV
                     IRDNAVADGSTANTLQVKVTDANGNTLAGQAVSVLAGNSATVASTVTTKPDGTVEISV
                     TSQTAGTSTVTASINSSSLSRNVTFVADVSTAKIADLVVIQDNSVADGAMANTLRMRV
                     TDAFGNTLGGQTVSVTADNSAMVASTVITGPDGTVEISVTSQTAGISIVTASINNSSL
                     SRDVTFVADVRTAKIADLVVIKDGSEADGSTANTLQVRVTDAFGNALAGQTVSVLADN
                     GATVAPTVTTQPDGTVEISVTSQTAGVSAVTATINSSTQSQNVTFIADVRTAKIADLV
                     VIKDGSEADGSTANTLRARVTDAFGNALAGQAVSVMAGNSATVTPTVTTQSDGTVEFS
                     VTSQTAGTSTVTASINSSSLSRDVTFIADVRTAQIAVLEVTQDYAVADGSTANTLRAR
                     VTDAFGNALAGQTVSVLGGNGATVSPTVITGPDGTVEISVTSQTAGASTVTASINSSS
                     LSRNVTFVADVRTAQIAVLEVTQDYAVADGSTANTLRARVTDAFGNALAGQTVSVTAG
                     NGATVSPTVITGPDGTVEISVTSQTAGVSAVTATINNSTASQNVMFIADVRTAKIADL
                     VVTRDNSVADGAMANTLQVKVTDANGNTLAGQTVSVLADNSATTAPTVITEPDGTVEI
                     SVTSQTAGTSTVTATINSSSQSQNVTFIADIRTAQIADLVVIKDGSVADGSTANMLRV
                     RVTDAFGNALGGQTVSVLADNGVTTAPTVITEPDGTVEISVTSQTAGVSAVTATINSS
                     SQSQNVTFIADVSTAKIADLVVIKDGSEADGSTANTLRVRVTDAFGNTLAGQTVSVLA
                     DNGATTAPTVITEPDGTVEISVTSQTAGVSAVTASINSSSQSRNVTFVADVRTAQIAD
                     LVVIKDGSEADGATANTLRARVTDAFGNALAGQTVSVLADNGATVAPTVTTQPDGTVE
                     ISVTSQTAGISAVTASINNSSLSRNVTFIADVSTAKIADLVVIKDGSEADGSTANTLQ
                     VKVTDANGNTLAGQTVSVLAGNSATVTPTVTTKPDGTVEISVTSQTAGISAVTASINS
                     SSQSRNVTFIADVRTAKIADLVVIKDDSVADGAMANMLRARVTDAFGNALAGQTVSVL
                     AGNSATVAPTMTTKPDGTVEISVTSQTAGTSTVTASINNSSQSQNVTFVPGDASQLTS
                     IVETNKSNYTVGETITITVTLRDAFDNLVTGAASQLAADGVLTVAGTDPSEMGSWVES
                     GGVYTTTRMATIASTNQHANLQLQTWSDGVTSDRYDIQSGSPAQATSTIATDKNAYTA
                     GDTITVAVTLKDAHGNLVEGGESLLSGDNVIVEGAVRSGGWSENAGVYTATWSAQMAG
                     DSHHATLKLSEWGSSKQSESYSIHSGAPVQANSAIRTDKSAYIAGEPLTVTITLRDEF
                     GNPALGLTSEVIESYIDSFAVGGATPDSMRWVEQNNGEYTIVWTAWVADENLVASLKL
                     KTWATEIKSSLYGIQPGAAAKTQSTIVADKTIYIAGDSITVTVVLKDAQGNFITDGVV
                     QLNEENVQVRNADPIQGNNWVYNGNGQYQRQYMAHFAEANLNAQLKMAGWSDANYSNN
                     YTIKPGEVSPLGSQLRIREVLVVEGADLPVSALLVDDFGNPVDNGLDLLDDAVYLQNV
                     EKKEGEKWRYVGDGIYERTYMAYQEGENLTSFMEIKGWRIYGQPSYTILPFVEVELLS
                     VNGVKFRATDGFPETGFDGAKFTLLLTHNMKNTDYNWTAGIYGINVDSNGEVTLSVLI
                     RSEVTITGKPKNGKGNDVVFKFKIKKWFTSLGATSSNTWDIINTSCSYGQMPSSLELA
                     QRPSGGVVPRKVGTLWGEYGNLKTYGNAFSGTDYWTSTQLMGVHEKFNPETGISELGT
                     GKSSGLCVEYY"
     gene            complement(15517..>15943)
                     /locus_tag="IU325_15920"
     CDS             complement(15517..>15943)
                     /locus_tag="IU325_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP5810487.1"
                     /translation="ISFALNLNSQNTCNNPIITSILAMFMALKGHNNKAKSLLLKLEP
                     EHGLDYTCVNSLYTKFLIYGASIKNDIMKLLANINTNKINGVILPLIYTVYGKKEYEK
                     RWQQLIKDNDLWSNVLLHDPRLLSVKNEFNTIGVMRTSA"
BASE COUNT         3896 a         3787 c         4608 g         3652 t
ORIGIN      
        1 tatccattaa tgtgcaattg aaatgtgata attatcactg aaccgttgtt gtcatttgtt
       61 ttacaaaagc aagggattaa atctcctcca atggacaaaa aaatgaataa tgacaatacc
      121 gattacttga gtaatgaatc agggacgctt tcgcgattat ttaaactacc tcagcatggg
      181 accaccgtcc gcacagaatt gattgcgggg atgaccactt ttttaaccat ggtgtacatc
      241 gtttttgtga acccgcaaat cctcggcgcg gcacaaatgg acccgaaagt ggtgtttgtt
      301 accacctgtt tgattgccgg tatcggcagt attgcgatgg ggatatttgc taacttaccc
      361 gtggcgctgg ctccggcaat ggggctgaac gccttctttg cgttcgtggt cgtgggggcg
      421 atgggtatct cctggcagac cgggatgggc gcgatattct ggggcgcaat tgggctattt
      481 ttgctcacgc tgtttcgtat ccgttactgg atgatctcca acattccatt aagtttacgt
      541 attggcatca ccagcggaat cggattattt attgccttaa tgggattaaa aaacactggc
      601 gttattgtcg ccaataaaga caccctggtg atgattggcg atttaagttc tcacggcgtg
      661 ttgttaggta ttttagggtt ttttattata accgtgttgt catcacgtca ttttcatgcc
      721 gcggtgcttg tttctattgt ggtgacgtct tgctgtggat tatttttcgg tgatgttcat
      781 tttagcggtg tctattccat tccgcctgat attagcggcg tcattggtga agtagatttg
      841 agcggcgcgt taacacttga actcgccggt atcattttct cctttatgct gatcaaccta
      901 tttgattcat caggaacatt aattggtgta actgataaag ctggcttaat agatagtaac
      961 ggtcaattcc ccaatatgaa taaagcgctg tatgttgata gcgtcagttc ggtggcaggc
     1021 gcatttattg gtacctcatc tgttactgcc tatattgaaa gtacttctgg tgtggcggtc
     1081 ggtggtcgca cggggctgac ggctgtcgtg gtcggcgtta tgttcctgtt agttatgttc
     1141 ttctcaccgc tggtggcgat ggttcctcct tacgcaaccg ccggagcgtt aatctttgtt
     1201 ggcgtgctga tgacttcgag cctggcgcgc gttaactggg atgattttac cgaatcggtg
     1261 cctgcgttta ttaccacggt gatgatgccc tttactttct cgatcaccga agggattgca
     1321 cttggcttta tgtcgtactg catcatgaaa gtatgcaccg ggcgctggcg cgatctgaac
     1381 ctgtgtgtgg tggtggtcgc agctctgttt gcactgaaga ttattttggt ggattagtaa
     1441 ctggcgaggg tttccagata tcatgagttc tgattacgca ggagaactca tgatctggat
     1501 aatgctcgcc acgctggcgg tagtgtttgt ggttggtttt cgggtgctga catccggggc
     1561 cagaaaagcg attcgccgtc tcagcgatcg gctgaatatc gatgtcgtac ccgtggagtc
     1621 gatggtcgat caaatgggaa agtcagccgg tgacgaattt ttacgttatt tgcatcgccc
     1681 ggatgagtcg cacctgcaaa acgccgcgca ggtattgctc atctggcaaa ttgtcattgt
     1741 cgatggtagc gaacagaacc tgctgcaatg gcatcggatt ttacaaaaag cccgccttgc
     1801 cgcgccgatt accgatgctc aggtcaggct ggcgctgggt tttctgcgcg aaaccgaacc
     1861 tgaaatgcag gatattaatg cttttcagat gcgctataac gcgttctttc agcctgccga
     1921 gggcgttcac tggctgcatt aaaaccgctt cgtcttatca gcctacagac cgcgcctgac
     1981 ctgtagggtg gataaggcgt tcacgccgca tccggtatcg gaactgacag ttggtgaacg
     2041 cattatctac gttctattta cagcagcacc tccagcccgt gtttttgcac aatgctgagc
     2101 agttttaatg aaatgccatt ggggcgttta gcgccacttt cccatttttg tacagttgaa
     2161 acgctggtat tgagatagtg agcgaagacg ggctgactca cttttaattt ttcccgcagt
     2221 gcttttatct caacaggttg taagtcttgc acgctggtca ggcaggcttt gtcaaaagta
     2281 cgcatcgttt cggcagaaat cgcatcgacg ctggacaatc cagccgctgc gctgtgaata
     2341 gcctcaaatg caggactttt aaatttagtt tttgcagtca tagcagatct ctgtcagttc
     2401 tttagtgtta atcattgctg tcagttgggc tttcgtcagg aaggcataat gctttgcaag
     2461 ttcacgaaac ccagccagtt cctgactatt aatgttcgac atatcctgtt ttgcatataa
     2521 aaacgtgtaa aaccaattga ttcccccttt cgccagaata attgcacgct cacgattttg
     2581 gtgaaggcgc tttttaaaaa cgccaccgcc aagtgcatca gctttacctt gttccgtttc
     2641 taaaatggcc cggcacaact cttcatctgt tattgcatga gacctggctt cccttgcaaa
     2701 ccatttggtt ttaaacacgc gcatatcgtt gagatcctgt tattaaagta tatcaccaag
     2761 tgcaatactt tatcccttgt tcccaacaca atcgcgaaaa tacatatcac cttgccctat
     2821 gatttttatt tcttcgagca gcctcgaact ttcccacaac accgtaaaag ttgcaaaaac
     2881 gttaaatgcg tcacacattt caatgttaaa gtggtcggct tttcccctga aacatgccac
     2941 gggtaacacc atgagtgaat ttattgccga aaaccgcggc gcggatgtca tcacccgacc
     3001 gaactggtca gccgttttct cggtggcgtt ttgcgtcgcc tgtctgatta tcgttgagtt
     3061 tttgcccgtc agtttgttga cgccaatggc ccaggattta ggcatttcgg agggggttgc
     3121 cgggcaatcg gtgaccgtga ccgcctttgt ggcaatgttt gccagtttgt ttattaccca
     3181 gacaattcag gccactgacc gccgctacgt tgttattttg tttgccgttt tgctgacgct
     3241 ctcctgtttg ctggtttcct ttgccaactc attcagtttg cttttaatcg gtcgtgcctg
     3301 tctggggctg gcgctgggcg ggttctgggc gatgtcggcg tcgctgacca tgcgtctggt
     3361 accgccgcgt acggtgccga aggcgctgtc ggtgatcttc ggcgcggttt ctattgcgct
     3421 ggtgattgcc gcgccgttgg gcagtttttt aggcgagctt atcggttggc gcaatgtctt
     3481 taatgcggcg gcggtgatgg gcgtgctgtg tattttctgg attatcaaat cattgccttc
     3541 actgccaggc gaaccctcgc atcagaaaca aaatactttc cgcttattac aacgtccggg
     3601 tgtgatggca gggatgatcg ccatcttcat gtctttcgcc gggcagtttg ctttcttcac
     3661 gtatattcgc ccggtgtata tgaacctggc gggattcggc gtggatggct taacgctggt
     3721 gctgttgagt tttggcattg ccagctttat tggtacgtcg ctttcgtcgt tcattcttaa
     3781 acgttcggta aaactggcct tagcaggcgc gccgttaata ctggctgtga gtgcgttggt
     3841 gctgacgttg tggggaagcg ataaaatcgt tgctaccggc gtggcaatta tctgggggct
     3901 aacttttgca ttggttccgg tcggctggtc aacgtggatc acccgctcac tggccgatca
     3961 ggcagaaaaa gccgggtcaa ttcaggtggc ggttattcag cttgctaata cctgtggcgc
     4021 ggcaatcggc ggttatgcgc tggataatat tggtctgact tcgccgttga tgttgtccgg
     4081 cacattgatg ttgctgacgg cgttattggt caccgcaaag gtgaaaatga agtaacgtat
     4141 ttacaggagg ggttactgct ctggcgggaa gctctcctgt tcttcccctg ataaccattg
     4201 atcaggtatt gttcttatct tgttgttatt tccattattc cgcctgtatt catcatttgc
     4261 taatcatgaa tcatcatttt ctctcatgtt aaagaaaggt catttaatct gaacgcatag
     4321 cgacagggat gacaaatggc agggaaagcg catgggaatg gcgatcgtcg gggcgataac
     4381 accatatgtg gattggggga tcgtttacgt cgtctgaccg caggtatttg cctgataaca
     4441 caaactattt tccctgttat ggctgctgcc cctacgcata ttaatcctgc gcactcggat
     4501 actgccgcgt cactgatcct gccgaacgtt aaaactattc catataccct gggtgcgttg
     4561 gaatcgccac caacggtcgc ggcacgcttt ggtatcaccg ttgatgaatt acgtcgcctg
     4621 aatcagttcc gtacctttgc gcggggcttt gacaatgtgc gtcagggcga tgaaatagat
     4681 gtgcccctca ttaacagtaa tagccccgaa gcgcgcaacc tgaaagcaat gcagatggag
     4741 cgcgacggca aagatccgca aatgcaggtc gcggaaatgg cgcagcaaag cggaacactt
     4801 ttagctcgcg atatggacag cgaacaggcg gcgagtatgg cgcgtggctg ggtggcttct
     4861 tctgcatctg cgcaggcaac ggactggctc agtcgttggg gaactgcccg ggtatctctt
     4921 ggtgtggatg aagatttcag ccttaaaagc tcgtcatttg aatttctcca tccctggtac
     4981 gaaacgccgg ataacctggt cttcagccag cacacacttc accgtacgga taatcgcact
     5041 caaaccaacc acggtatcgg ctggcgttat ttcacatcga gctggatgtc tggcgtcaat
     5101 atgtttatcg atcatgacct gacccgctat cacacccgaa ccgggatggg cgttgaatac
     5161 tggcgcgatt acctgaaact gagcggcaac ggttatctgc gcctgagtaa ctggcgtagt
     5221 gcaccggaac tcgacaatga ctatgaagca cgtccggcga acggttggga tcttcgcgcc
     5281 gaaggctggt taccggcctg gccgcagctg ggcggcaagg tggtctatga acaatattat
     5341 ggcgatgaag tggcgctgtt tggtaaagac gagcgtcaaa atgatcctca cgcgattacc
     5401 gcaggtttga gttatacccc tgttcctctg atctccttta gtgccgagca acggcagggc
     5461 aaacagggag aaaacgacac ccgaattggt atggagttga cgttgcaacc cggccattca
     5521 ctgcaaaaac agctcgatcc ggctgaagtt gcagcacgac gtagccttgt gggcagtcgc
     5581 tatgacctgg tcgatcgtaa taacaatatt gtcctcgaat atcgcaagaa agagctggtg
     5641 cggctgacgc tgaccgatcc gttgaaaggc aagccgggtg aggttaaatc gctggtttct
     5701 tccttacaaa ccaaatatgc gctgaaaggt tatgacattg aagccgcctc cctgcaatca
     5761 gcgggcggaa aagtagctgt ttccggcaag gatattcagg tcactattcc tccctaccga
     5821 ttcacggcaa tgcctgaaac cgacaacact tacccgatag cggttacagc ggaagattcg
     5881 aaaggaaatt tctcccggcg tgaagaaagt atggtggtcg tggaaaaacc cacattaagc
     5941 ctcgccggtt caacgctttc tgtcgatctg caaatcctgc ttgctgatgg aaaatcaacc
     6001 tcaacgctga cctataccgc gcgagattcc agcggcaagc caatcccagg tatgacgtta
     6061 aaaacacagg tgaaaggttt gcaggatttc gccttaagcg aatggaaaga caacggtaac
     6121 ggaacctata cccaaatagt taccgccggg aagacgtctg gcgcgttatc gctgatgccg
     6181 caatttaacg gcgatgatat cgctaagacg ccagcactga ttgctatcgt ggcgaataca
     6241 gcatcccgcg ctgactccac gattgagaca gaccaggata attacgtcgc gggtaagccg
     6301 atagtcgtta aagtgaccct gagagatgat aacggcaacg gggttaccgg acgaaaagaa
     6361 cttctcaaac aaaccgttaa agttgacaac acgaaggctg atgccgttag tgcctggacg
     6421 gaagaaagtg agggaattta caaagcaagc tatacggccc atctcattgg cgacaaatta
     6481 acggcccagc tgacgatgcc cggctggcaa accaaacatt cggatgcgtt cagtattgcg
     6541 ggtgataaag ataccgccaa aatcgctgcg atgcagatca ccgcgaataa cgcagttgcc
     6601 agacgcgatc acaataccgt cgctgtgacg gtcagggatg tgcatcaaaa tttactgcag
     6661 ggacaaaatg ttaccttcac ggtcgtgaat ggtgcggcgg tttttgccga tccaaatggc
     6721 ggtattgtga cgacagataa agacgggatt gccagcgtta accttgccag tgaccaggcg
     6781 gtaaacagtc ttatcaaggc cgagaccaat ggcagtagtc agtctgtgga agtgagcttt
     6841 attactggcg atatctctca gctgacctcc accatcaaga cagacgatgt gtcatacacc
     6901 gcgggtggca agataaaagt gtcagttacc cttatggatg agcagaaaaa cctggtgaaa
     6961 gggatggctt cgctgctggc gggtagcggt gtggtcgagg tgagcggtac agataaaaat
     7021 gaaacgggta actggagcga agagtctgat ggcgtttaca ccaccacccg aaccgcgaag
     7081 attgctggcg atcgccatta cgccacgctg aaactatcga catggagtag cgcgcagcaa
     7141 tccgatgcgt atgccatccg cgaaagcgga gctgtgttgg cttattcctc catcgttacc
     7201 gacaaaacga cctacaccgc gggcggcgcg ataaaagtta cggtaacact caaggatagc
     7261 tatgaaaacc tggtcggggg gcaacgagac gctatcaacc tggctatcca gctgccaaat
     7321 acaaaagcgg aaagcatcgc ctggaatgaa gatcaaaaag gaatttatac cgcaacgtat
     7381 accgccttac tccctggcac cggtttaaaa gcacagctac agatgtccgg ctgggcgaac
     7441 gctctgactt caaatgatta cagtattagt ggcgatgccg ccagcgcaca aattgtcgcg
     7501 atgcaagtga caacgggtaa cccggatgtg ctggcgaatg gcagcgatcg acatacagtg
     7561 aatgtccggg tggaagatca gtttggcaat gtgctgccgg agcagaccgt cacctttacg
     7621 gtgacgaagg gggcggctgt atttgccaat gcagggcaga gcgcaggtat caggacggat
     7681 gcgcatggca tggcagaggt tgatctgagc agtaccgtag cggatgccag cactgttgag
     7741 gctaagatca atcaaagcag cgatagtaaa acggtgaact ttgttgctga tgttagcacg
     7801 gcgcaggtcg cggagctggt ggtcattaag gatggttcag aggcggacgg ttcgacggcg
     7861 aacacgctgc gggtgaaggt caccgatgcg ttcggtaata cgcttgccgg gcagacggtc
     7921 agcgtgttgg cggacaacgg tgcaacggtc gctccgacgg tgattacaga gccggacggc
     7981 acggtggaga tctcagtcac cagccagacc gccggaacca gtgtggtcac cgccagcgtc
     8041 aacaacagca gccagagccg gaacgtgacg tttgtcgccg atgtcaggac ggcgaagatc
     8101 gctgatctgg tagtcacccg ggataactca gtggcagacg gtgcaatggc gaacacgctg
     8161 cgggtgaggg tcacagatgc gttcgggaat acgcttgccg ggcagacggt cagcgtgatg
     8221 gcagacaaca gcgcaacggt gtctccgacg gtcactacag agccggacgg cacggtggag
     8281 atcagtatca ccagccagac agccggaacc agtacaggca ctgccagcat caacaacagc
     8341 agcctgagcc ggaacgtgac gtttattgcg gatgtcagga cggcgaagat cgctgatctg
     8401 gtggtcatta aggacgactc agtggcagat ggtgtaatgg cgaatatgct gcgggcgagg
     8461 gtcaccgatg cgttcgggaa tgtacttgcc ggccagacgg ttagcgtgac ggcggacaac
     8521 ggcgcaacgg ttgctccggt ggtcatcaca gggccggacg gcacggtgga gatctcagtc
     8581 accagccaga ccgccggaac cagtgcgatc accgccagta tcaacaacag cagcctgagc
     8641 cggaacgtga cgtttgtcgc cgatgtcagg acggcgaaga tcgctgatct ggtagtcacc
     8701 cgggataact cagtggcaga cggtgcaatg gcgaacacgc tgcgggtgag ggtcacagat
     8761 gcgttcggga atacgctgaa tgggcagacg gtcagcgtgt tggcagacaa cggtgccacg
     8821 actgctccga cggtcaccac gcagccggac ggcacggtgg aaatcagtgt caccagccag
     8881 acggcgggag tcagtactgt cactgccagc atcaacagca gcagcctgat ccggaacgta
     8941 acgtttgtcg cggatgtcag gacggcgcag atagcgagtc tggaggttac ccgggataac
     9001 tcagtggctg acggcgcaat ggcgaacacg ctgcgggtga aggtcaccga tgcgttcggg
     9061 aatgcgctga atggacagac ggtcagcgtg atggcggaca acggcgcaac ggttgccccg
     9121 acggtgatca ccgagccgga cggcacggtg gaaatcagtg tcaccagcca gacggcggga
     9181 gtcagtgcag tcactgcaac catcaacagc agtagtcaga gtcagaacgt gacatttatc
     9241 gccgatgtca gcacggcgaa gatcgctgat ctggtggtca ttaaggatgg ttcagaggca
     9301 gacggttcga cggcgaacac gctgcaggtg aaggtgaccg atgcgttcgg gaatgcactt
     9361 gccggacaga cggtcagcgt gatggcaggc aacggtgcca cgactgctcc gacggtcacc
     9421 acgcagccgg acggcacggt ggagatcagt gtcaccagtc agaccgccgg agccagtaca
     9481 gtcaccgcca gcattaacaa cagcagcctg agccagaacg tgacgtttgt cgccgatgtc
     9541 agcacggcga agatcgctga tctggtggtc attaaggatg gttcagaggc agacggttcg
     9601 acggcgaaca cgctgcgggc gagggtaacc gatgcattcg gtaatgcact tgccggacag
     9661 acggtcagcg tgatggcagg caacggcgca acggttgccc cgacggtgat caccgagccg
     9721 gacggcacgg tggaaatcag tgtcaccagt cagacggcgg gaatcagtgc agtcacagcc
     9781 agcatcaaca gcagcagtca gagccgggac gtgacattta tcgccgatgt caggacggcg
     9841 aagatcgctg aactggaggt catccgggat aacgcggtgg cagacggttc gacggcgaat
     9901 acgctgcagg tgaaggtcac cgatgctaac ggtaatacgc ttgccggaca ggcggtcagc
     9961 gtgttggcag gcaacagcgc aacggttgct tcgacggtga ccacaaagcc ggatggcacg
    10021 gtggagatca gtgtcaccag tcagacggca ggcaccagta cagtcactgc cagcatcaac
    10081 agcagcagcc tgagccggaa cgtgacgttt gtcgccgatg tcagcacggc aaaaatagcg
    10141 gatctggtcg ttatccagga taactcagtg gctgacggcg caatggcgaa cacgctgcgg
    10201 atgagggtca cggatgcatt cggtaatacg cttggcggac agacggtcag cgtgacggca
    10261 gacaacagtg caatggtcgc ttcgactgtc atcacagggc cggacggtac ggtggagatc
    10321 agtgtcacca gccagacggc ggggatcagt attgtcactg catccatcaa caacagcagc
    10381 ctgagccggg acgtgacgtt tgttgccgat gtcaggacgg cgaagatcgc tgatctggtg
    10441 gtcattaagg atggttcaga ggcggacggt tcgacggcga acacgctgca ggtgagggtc
    10501 accgatgcgt tcggtaatgc gcttgccggg cagacggtca gcgtgttggc ggacaacggc
    10561 gcaacggtcg ccccgacggt caccacgcag ccggacggta cggtggaaat cagtgtcacc
    10621 agccagacgg cgggagtcag tgcagtcact gcaaccatca acagcagtac tcagagtcag
    10681 aacgtgacat ttatcgccga tgtcaggacg gcgaagatcg cggatctggt ggtcattaag
    10741 gatggttcag aggcggacgg ttcgacggcg aacacgctgc gggcgagggt gaccgatgcg
    10801 ttcggcaatg cacttgccgg gcaggcggtc agcgtgatgg caggcaacag cgcaacggtc
    10861 actccgacgg tcaccacgca gtcggacggt acggtggagt tcagtgtcac cagccagacg
    10921 gcaggaacca gtacagtcac cgccagtatc aacagcagca gcctgagccg ggacgtgacg
    10981 tttattgcgg atgtcaggac ggcgcagatc gcggtgctgg aagtgacgca ggattacgcc
    11041 gtggcagatg gttcgacggc gaacacgctg cgggcgaggg tgaccgatgc gttcggtaat
    11101 gcgcttgccg ggcagacggt cagcgtgttg ggaggcaacg gcgcaacggt gtctccgacg
    11161 gtcatcacag ggccggacgg cacggtggag atcagtgtca ccagtcagac ggcgggagcc
    11221 agtactgtca ctgccagcat caacagcagc agcctgagcc ggaacgtgac gtttgtcgcg
    11281 gatgtcagga cggcgcagat cgcggtgctg gaagtgacgc aggattacgc cgtggcagat
    11341 ggttcgacgg cgaacacgct gcgggcgagg gtgaccgatg cgttcgggaa tgcacttgcc
    11401 gggcagacgg tcagcgtgac ggcaggcaac ggcgcaacgg tgtctccgac ggtcatcaca
    11461 gggccggacg gcacggtgga aatcagtgtc accagccaga cggcgggagt cagtgcagtc
    11521 actgcaacca tcaacaacag caccgcgagc cagaacgtga tgtttatcgc cgatgtcagg
    11581 acggcgaaga tcgcggatct ggtggtcacc cgggataact cagtggctga cggcgcaatg
    11641 gcgaacacgc tgcaagtgaa ggttaccgat gctaacggta atacgcttgc cgggcagacg
    11701 gtcagcgtgt tggcggacaa cagcgcaacg actgccccga cggtaatcac agagccggac
    11761 gggacggtgg agatctcagt caccagccag accgccggaa ccagtactgt cactgcaacc
    11821 atcaacagca gtagtcagag tcagaacgtg acgtttatcg ccgatatcag gacggcgcag
    11881 atcgctgatc tggtggtcat taaggacggc tcagtggcag acggttcgac ggcgaacatg
    11941 ctgcgggtga gggtgacgga tgcgttcggt aatgcgcttg gcggccagac ggtcagcgtg
    12001 ttggcggaca acggcgtaac gactgcccca acggtgatca ccgagccgga cggcacggtg
    12061 gaaatcagtg tcaccagcca gacggcggga gtcagtgcag tcactgcaac catcaacagc
    12121 agtagtcaga gtcagaacgt gacatttatc gccgatgtca gcacggcgaa gatcgctgat
    12181 ctggtggtta ttaaggatgg ttcagaggcg gacggttcga cggcgaacac gctgcgggtg
    12241 agggtgaccg atgcgttcgg gaacacgcta gccgggcaga cggtcagcgt gttggcggac
    12301 aacggcgcaa cgactgcccc gacggtcatc acagagccgg acggtacggt ggagatctca
    12361 gtcaccagcc agacggcggg agtcagtgca gtcactgcca gcatcaacag cagcagccag
    12421 agccggaacg tgacgtttgt cgccgatgtc aggacggcgc agatcgcgga tctggtggtt
    12481 attaaggacg gttcagaggc ggacggtgcg acggcgaaca cgctgcgggc gagggtgacc
    12541 gatgcattcg gtaatgcgct tgccgggcag acggtcagcg tgttggcgga caacggcgca
    12601 acggtcgccc cgacggtcac cacgcagccg gacggtacag tggagatctc tgttaccagc
    12661 cagacggcgg ggatcagtgc ggtcacagcc agcatcaaca acagcagtct gagccggaac
    12721 gtgacattta tcgccgatgt cagcacggcg aagatcgctg atctggtggt cattaaggat
    12781 ggttcagagg cagacggttc gacggcgaat acgctgcagg tgaaggtcac cgatgctaac
    12841 ggtaatacgc ttgccggaca gacggtcagc gtgttggcag gcaacagcgc aacggtcact
    12901 ccgacggtga ccaccaagcc ggacggcacg gtggagatat ctgtaaccag ccagacggcg
    12961 ggaatcagtg cagtcactgc cagcatcaac agcagcagtc agagccggaa cgtgacgttt
    13021 atcgccgatg tcaggacagc gaagatcgcg gacctggtgg tcattaagga cgactcagtg
    13081 gcagacggtg caatggcgaa tatgctgcgg gcgagagtca ccgatgcgtt cggtaatgcg
    13141 cttgccgggc agacggtcag cgtgttggcg ggcaacagcg caacggttgc tccgacgatg
    13201 accacaaagc cggatggcac ggtggagatc agtgtcacca gccagacggc gggcaccagt
    13261 acagtcaccg ccagcatcaa caacagcagc cagagccaga acgtcacctt tgtacctggt
    13321 gatgcatcgc aactcacttc aatcgttgaa acaaataagt cgaactatac ggttggagag
    13381 acaatcacca tcacggtaac gctccgggat gcgttcgata atctggtaac cggtgcagct
    13441 tcacagttag ccgctgatgg tgtgctgacg gtggctggca ctgacccgtc agaaatggga
    13501 agctgggttg aatcaggtgg cgtgtatacc acaacccgaa tggcgacgat tgccagtacc
    13561 aatcagcacg ccaacctgca attgcagacg tggagtgatg gcgtaacgtc agatcgctat
    13621 gacatccagt ccggttctcc ggcgcaagcc acatcaacta tcgctacgga taaaaacgcc
    13681 tacaccgctg gcgacactat aaccgtcgcc gtgacgctga aagacgcgca cggtaacctg
    13741 gttgaagggg gcgagtcctt actgtctggt gataacgtaa tagtggaagg agccgtacgt
    13801 tcaggaggat ggtctgaaaa cgccggtgtt tatactgcta catggtcggc gcaaatggcg
    13861 ggagactctc accacgcgac gctgaagttg tctgaatggg gcagcagtaa acaatcagag
    13921 agttattcca tccacagcgg tgctccggtg caggcaaatt ctgctatcag gacagataaa
    13981 tcggcatata tcgccgggga gccattaacc gtcacaataa cgttgcggga tgagtttggt
    14041 aaccctgctt tagggctgac atcagaagtc atcgagtctt atattgatag tttcgcggta
    14101 ggcggcgcaa ctcccgattc tatgcggtgg gtcgagcaga ataatggtga atataccatt
    14161 gtctggacag catgggttgc cgacgaaaat ctggttgcca gtctgaaatt aaaaacatgg
    14221 gcaacggaga ttaagtcctc gctatacggg atacaaccag gtgcagcggc aaaaactcaa
    14281 tcaacgattg tcgcggacaa aacgatatat atcgctgggg atagcataac ggtgaccgtt
    14341 gttcttaaag atgcccaggg taactttatt actgatggtg ttgttcagct taatgaggaa
    14401 aacgtacaag tcaggaatgc ggatcctatc cagggcaata attgggttta caacggcaat
    14461 gggcaatatc aaagacagta tatggcgcat tttgcggaag cgaatttgaa tgcgcaactg
    14521 aagatggctg gttggagcga tgccaattat tcaaacaatt acactattaa accaggtgaa
    14581 gtgagtccgc ttggttcaca gcttcgtata cgagaagttt tagtagtgga aggggcggat
    14641 ttacctgtca gtgctttatt agttgatgat tttggaaatc cggtggataa cggtctggat
    14701 ttgctggatg atgcagtgta cttacaaaat gtagaaaaaa aagaagggga aaaatggaga
    14761 tatgtgggtg atggaatata tgaacgtaca tatatggcct accaagaagg agaaaattta
    14821 acttcattta tggagattaa aggttggcgt atatacggac aaccatccta taccatcctt
    14881 ccttttgtgg aagtcgagtt actgagtgtt aatggtgtaa aattcagggc gacagatggt
    14941 tttccagaaa cagggtttga tggtgcaaaa ttcacgctgt tactaaccca taatatgaaa
    15001 aatacggatt ataattggac ggctgggatt tatggaatta atgttgacag taatggagaa
    15061 gtaacattat ctgtgctgat aagaagtgaa gttactatta ctgggaaacc caagaatggt
    15121 aaagggaatg atgttgtatt taaattcaag attaaaaaat ggtttactag tttaggtgct
    15181 accagtagta atacctggga tatcataaat acttcatgta gttatggtca aatgccgtca
    15241 tctttagaat tagcgcagcg gccaagcggt ggggtagtac cgcgaaaagt aggtacgttg
    15301 tggggcgaat atggtaattt aaaaacttat gggaacgctt ttagcggtac ggattattgg
    15361 acaagcactc agctcatggg agtacacgag aaatttaatc ctgaaactgg tatatcagag
    15421 cttgggacag gaaagtcttc tggcttgtgc gtagagtatt attaacgaag taattactaa
    15481 attgccctgc atcagcaggg caatttatat tcgttattac gcagaagtac gcatcacacc
    15541 gatagtatta aattcatttt ttacactgag caaacggggg tcatgaagca aaacatttga
    15601 ccataaatca ttgtctttaa ttaattgttg ccagcgcttt tcatattctt ttttaccata
    15661 cacagtgtaa atcaacggca aaatcacacc attgattttg ttggtgttaa tatttgccag
    15721 taacttcata atgtcatttt ttatagatgc gccatagatc agaaatttcg tataaagcga
    15781 gttaacacag gtgtaatcaa gaccatgttc aggctcaagt ttaagcaata aacttttggc
    15841 tttgttatta tgtcctttca gtgccatgaa catagccaga atagaggtaa taattggatt
    15901 attacaggtg ttttgactgt taagatttaa tgcaaaagat atc
//
LOCUS       DACBVI010000104        15626 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.104, whole genome shotgun sequence.
ACCESSION   DACBVI010000104 DACBVI010000000
VERSION     DACBVI010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.104"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            28..813
                     /gene="caiD"
                     /locus_tag="IU325_15925"
     CDS             28..813
                     /gene="caiD"
                     /locus_tag="IU325_15925"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HAP5810488.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMSAEEALRWGVVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            819..1409
                     /gene="caiE"
                     /locus_tag="IU325_15930"
     CDS             819..1409
                     /gene="caiE"
                     /locus_tag="IU325_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HAP5810489.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLIGTPARAVRNVSDEELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(1528..1923)
                     /gene="caiF"
                     /locus_tag="IU325_15935"
     CDS             complement(1528..1923)
                     /gene="caiF"
                     /locus_tag="IU325_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HAP5810490.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(2184..5402)
                     /gene="carB"
                     /locus_tag="IU325_15940"
     CDS             complement(2184..5402)
                     /gene="carB"
                     /locus_tag="IU325_15940"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP5810491.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNADFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECESNPSDREKIMVLGG
                     GPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLED
                     VLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAVE
                     RLKLKQPANATVTTIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQTA
                     VSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPAY
                     TLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKAT
                     GVPLAKVAARVMAGKTLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRSTG
                     EVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELDA
                     THGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIRR
                     SALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(5420..6568)
                     /gene="carA"
                     /locus_tag="IU325_15945"
     CDS             complement(5420..6568)
                     /gene="carA"
                     /locus_tag="IU325_15945"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP5810492.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAETYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(7024..7845)
                     /gene="dapB"
                     /locus_tag="IU325_15950"
     CDS             complement(7024..7845)
                     /gene="dapB"
                     /locus_tag="IU325_15950"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP5810493.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(8012..8926)
                     /gene="rihC"
                     /locus_tag="IU325_15955"
     CDS             complement(8012..8926)
                     /gene="rihC"
                     /locus_tag="IU325_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HAP5810494.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(8992..9942)
                     /gene="ispH"
                     /locus_tag="IU325_15960"
                     /gene_synonym="lytB"
     CDS             complement(8992..9942)
                     /gene="ispH"
                     /locus_tag="IU325_15960"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HAP5810495.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(9944..10393)
                     /gene="fkpB"
                     /locus_tag="IU325_15965"
     CDS             complement(9944..10393)
                     /gene="fkpB"
                     /locus_tag="IU325_15965"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAP5810496.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(10481..10975)
                     /locus_tag="IU325_15970"
     CDS             complement(10481..10975)
                     /locus_tag="IU325_15970"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HAP5810497.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(10975..13791)
                     /gene="ileS"
                     /locus_tag="IU325_15975"
     CDS             complement(10975..13791)
                     /gene="ileS"
                     /locus_tag="IU325_15975"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP5810498.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKELLGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLFAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(13834..14775)
                     /gene="ribF"
                     /locus_tag="IU325_15980"
     CDS             complement(13834..14775)
                     /gene="ribF"
                     /locus_tag="IU325_15980"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HAP5810499.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(14783..15001)
                     /locus_tag="IU325_15985"
     CDS             complement(14783..15001)
                     /locus_tag="IU325_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 domain-containing protein"
                     /protein_id="HAP5810500.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            15104..15367
                     /gene="rpsT"
                     /locus_tag="IU325_15990"
     CDS             15104..15367
                     /gene="rpsT"
                     /locus_tag="IU325_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP5810501.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         3667 a         4329 c         3992 g         3638 t
ORIGIN      
        1 ctgaaaaagc aaaaatggag aaaaggaatg agtgaatcat tacatctgac ccgcaatgga
       61 tcaattctgg aaattaccct tgatcgccca aaagcgaatg ctattgatgc aaaaaccagc
      121 tttgaaatgg gcgaagtatt tctaaatttc cgtgacgatc cgcaattacg tgtcgccatt
      181 attaccggtg ccggagagaa attcttttcc gcgggctggg atttaaaagc ggcagcagaa
      241 ggcgaagcac cggatgctga ctttggcccg ggtggttttg cgggattaac cgaaattttc
      301 aatctcgaca aaccagttat cgccgctgtg aacggctacg cctttggcgg cggctttgaa
      361 ctggcactgg cggcagattt tattgtctgt gccgataacg ccagcttcgc cctgccggaa
      421 gccaaactgg gcatcgttcc tgacagcggc ggcgtgctgc gtctgccgaa gatcctgccg
      481 cctgccatcg tcaatgaaat ggtgatgacc ggcagacgaa tgagcgcaga agaggcgctg
      541 cgttgggggg tagtcaaccg cgtggttagc caggcggaac tgatggataa cgcccgcgaa
      601 ctggcgcagc agctggttaa cagcgctccg ctggcgattg cggcgctgaa agagatctac
      661 cgcaccacca gcgaaatgcc ggtagaagaa gcgtatcgct atattcgcag cggcgtgttg
      721 aaacactatc cgtcggttct gcattcggag gatgccattg aagggccgct ggcgtttgcc
      781 gagaagcgcg atccagtgtg gaaaggacgt taacaaccgt gagctattac gcctttgagg
      841 ggttaattcc ggttgttcac ccgacggcgt ttgtccatcc cagtgcagtc ttgattggcg
      901 atgtgattgt gggagccggt gtctacatcg gcccactcgc ctcactgcgt ggtgactacg
      961 ggcggttgat cgtgcaagcg ggagccaata ttcaggatgg ctgcattatg catggctact
     1021 gcgacactga caccatcgtt ggggaaaacg gccatatcgg gcacggagcg atcctgcatg
     1081 gttgtgtgat tggtcgcgat gcactggtcg ggatgaacag cgtgattatg gatggcgcgg
     1141 tcattggcga agagagcatt gttgccgcca tgagctttgt caaagcaggc tttagcggcg
     1201 agaaacgcca gttgttgata ggtacgccag cccgcgctgt acgcaatgtt agtgacgaag
     1261 agttacactg gaaacggttg aataccaaag agtatcagga tcttgtcggg cgctgtcatg
     1321 catcgttgca tgaaacgcag ccattgaggc aaatggagga aaatcgcccc cgtttgcagg
     1381 ggacgacgga tgtgacgccg aaacggtgat cttgccggat gcggcgtaaa cgccttatcc
     1441 gacctggctg gaaagcaacc gtaggccgta taagatgcgc cagcgtcgca tccggcgacc
     1501 aggtcgcacg gtatcaacga aacgacatta acgacgcata ctctttgaca acatcatctg
     1561 ccacttctgc tcgcggttaa gttccgtgag cggaacggca ggaatacgcg gcgtctttct
     1621 tacgccgact aatttttccc gactgttatt gcgcaggcgc gcgtaaattt gctcatcgat
     1681 atcgaccact ttaaccagtc gctgacactg gcatccccgc ccttccagct tattagggat
     1741 catcttaact tcgcagctta tttctgtgac ttccgacaga atataagtca gggtattaac
     1801 cgccttgctg tgttcaatat cgaaatgaat agagatctct cttgctatca cccaccgatt
     1861 ttcagccatc atccattcgg cgattaacaa gtagagtggt ttttcaacat atccttcaca
     1921 catacgaact ccagtttcac aaattaaaag tctggctatg actcgttttg aagcaattaa
     1981 acaaggccat attaaaattg tggatatatc gatagtatgc aacttcaccg tcgataagtg
     2041 tgagctaaat cctgcttatg tcatcaaaaa tatcgattcc ccaaaagatt tacttaatgg
     2101 gcaaaaaaaa cggtcctcat cagagaaccg tattagttat tcgatcaaat tagcggatga
     2161 aaaatatccg ccatgacacg ctattatttg atctgcgcgt gcatttcctg caccgaaatt
     2221 actttttcag tcgcatcggc attcagcgcc atcgcggtag cgaaaccacc gttcagggtg
     2281 gtgtcataat gcactttata ttgcagcgca ctgcgacgga tcacgcggga gtcttcaatc
     2341 gcacgacggc ctgaggtggt gttgatgatg taggtatatt cgccattctt gatacggtcc
     2401 tgaatgtgcg gacgaccttc atgcaccttg ttcaccagac gcggattaat gcctgcttcg
     2461 cccagcacaa tcgccgtgcc gtgggtcgca tccagctcga agccctgttt cagcagtttt
     2521 gccgccaggt ccaccacgcg ttctttgtcg ccttcgcgca cggaaagcag cgcacgaccg
     2581 tgtttcttca tggtggagtt gctgcccagc tgcgctttgg cgaacgcttc agcgaaggtg
     2641 cggcccacgc ccatgacttc cccggtagag cgcatttctg gccctaacag cgggtcaacg
     2701 cccgggaatt tattgaacgg cagcaccact tctttcaccg agtagtacgg agggataact
     2761 tctttggtta cgccctgctc ggccagcgtt ttgcctgcca tcacgcgcgc cgccactttc
     2821 gccagcggta cgccggtggc tttggagacg aacggaacgg tacgcgctgc acgcgggtta
     2881 acttcaatca ggtagacttc gttgtttttc accgcaaact gcacgttcat caggccgcgt
     2941 acctgcaatt cgaaggccag tttctgcacc tgctggcgca tcacatcctg aatttcctga
     3001 cttaaggtgt aggctggcag cgaacatgcg gagtcaccgg agtgcacgcc cgcctgctca
     3061 atatgctcca tgatgccgcc aatcagcacc atttcgccgt cgcagatagc atccacgtca
     3121 acttctactg cgtcatcaag gaagtggtcc agcaacactg gcgcatcgtt agatacgctg
     3181 accgctgtct ggaagtagcg acgcaggtca gcttcgtcat agacgatttc catcgcccga
     3241 ccgccgagaa cgtaagacgg acgcaccacc agcgggtagc caatctcttt cgctttctca
     3301 accgccattt cgatggtggt aacggtggcg ttcgccggtt gtttcagttt cagacgctca
     3361 accgcatgct ggaagcgttc acggtcttcc gcacggtcga tggcatccgg gctggtaccg
     3421 ataaccggta cgccagcagc ttccagcgcg cgcgccagtt tcagcggggt ctgaccgccg
     3481 tactggacga taacgccttt cggcttctca atacgcacaa tttccagcac gtcttccaga
     3541 gttaccggct cgaagtagag gcggtcggaa gtgtcgtaat cagtggagac ggtttccggg
     3601 ttacagttaa ccataatggt ttcgtaaccg tcttcgcgca gcgccagtga ggcgtgtacg
     3661 caacagtagt cgaattcgat accctgaccg atacggttcg ggccgccgcc gaggaccatg
     3721 attttttcac ggtcagatgg gttggattcg cactcttctt cataagtgga gtacatataa
     3781 gcggtgtcgg tagcgaactc tgccgcacag gtatccacgc gcttgtaaac cgggtgcagg
     3841 tcatactggt cacgcagctt acggatttct gcttcacgca cgcccgcgag ttttgccaga
     3901 cgcgcgtcgg caaagccttt gcgtttcaac tggcgcagga agtcagcgtt caggccagtg
     3961 atgcccactt ccgccacttt ctcttccaga cgcaccagct cttcaatctg taccaggaac
     4021 cagcggtcaa tgttggtcag gttgaagacg ccgtctacgg acaggcccgc gcggaaagca
     4081 tcggcgatat accaaatacg ctctgcgcca gcgtctttca gctcgcggcg gattttagtc
     4141 agcgcttccg ggtcgtcgag actcactttc ggatcgaagc cggtcgcgcc cacttccagg
     4201 ccgcgcagcg ctttttgcag ggattcctgc tgcgtgcgac caatcgccat cacttcgcca
     4261 accgatttca tctgagtggt cagacggtcg ttcgccccgg cgaatttctc gaagttgaag
     4321 cgaggaattt tggtgaccac gtagtcgatg gacggctcga aggaggccgg agtacgcccg
     4381 ccggtgatgt cgttcatcag ctcgtcgagg gtgtaaccca ccgccagttt cgccgccact
     4441 ttggcaatcg ggaaaccagt ggctttcgac gccagcgccg aagaacgcga cacgcgcggg
     4501 ttcatttcga taacaatcag gcgaccgttt ttcgggttca ccgcaaactg gacgttggaa
     4561 ccaccggttt caacgccgat ttcacgcagc accgccatcg aggcgttacg catgatttga
     4621 tattctttgt cggtcagcgt ttgggctggt gcgacagtga tggagtcacc ggtgtggatg
     4681 cccattgcat cgaagttttc gatagagcag acgatgatgc agttgtcgtt tttatcacgc
     4741 accacttcca tctcgtactc tttccagccg atcagcgact catcaatcag cagttctttg
     4801 gtcggagaga gatccagacc gcgggcgcaa atttcttcaa actcttcgcg gttataagcg
     4861 ataccgccac cgctaccacc catggtaaag gatgggcgaa taatgcacgg gaagcccacg
     4921 tcagcggcaa ccgccagcgc ttcttccatc gtgtgtgcga taccggaacg cgcggtttcc
     4981 agaccaattt tcttcatcgc tacgtcgaaa cgacggcggt cttctgcttt atcaatcgca
     5041 tcggcagtgg caccaatcat ggtgacgccg aactcttcca acacgccctg acgttccagc
     5101 tccagcgcac agttcagcgc cgtctgaccg cccatcgttg gcagcaccgc gtccgggcgc
     5161 tctttttcaa taatcttgcg taccacttcc cagtgaatcg gctcgatgta ggtcgcatca
     5221 gccatttccg ggtcggtcat gatggtcgcc gggttggagt tcaccagaat gacgcggtaa
     5281 ccctcttcac gcagggcttt acacgcttgc gcgccagagt agtcaaactc acacgcctga
     5341 ccgataacaa tcgggcccgc acccagaatc aggatacttt ttatatctgt acgttttggc
     5401 atggctcttt tactcctgat tacttagcgg ttttacggta ctgctcaatt aactcgataa
     5461 agtggtcgaa caacggcgct gcgtcgtgtg gcccagggct ggcttcaggg tgaccctgga
     5521 agctgaacgc cggtttatcg gtgcgatgaa tgccctgtaa cgtaccgtcg aacagggatt
     5581 tatgcgtgac acgcaggttt gcaggtaatg ttgcttcatc caccgcaaaa ccgtggttct
     5641 gggcggtgat catcaccacg tttttctcca catctttaac cggatggttg ccgccgtggt
     5701 gaccaaattt cattttgaca gtcttcgcac cgctcgccag cgccagcagc tgatgaccga
     5761 ggcagatgcc gaagaccgga atatcggttt cgaggaattt ctggatggcg gtaatggcgt
     5821 aatcgcacgg ggccgggtcg ccaggaccgt tggagaggaa gatgccgtct ggattcattt
     5881 tcaacacatc ttccgcagaa gtttgcgccg gaacgatggt caggcggcag cctctatcca
     5941 ccaacatgcg caggatgttg cgcttggcac caaaatcata agccacaaca tggaacggca
     6001 gctcgtcttc tttcttcgct tctggcaggc cgccggtcag cgtccagctc ccttgtgtcc
     6061 agctatacgt ttctgcggtg gtcacttctt ttgccagatc catgccgttc agacctggga
     6121 acgcgcgggc tttttctaac gccagcgccg catccgggtt atcgcccgcg ataatgcagc
     6181 cattctgtgc gcctttctcg cgcagtaaac gcgtcagctt acgggtatcg atatcggcaa
     6241 tcgccacgat gttatggcgc ttcaggtaag aagagaggtc ttcggtatta cggaagttgc
     6301 tggcaatcag cggcaggtcg cgaatcacca gaccttgtgc atgtacctga gaagattctt
     6361 catcggcgtc attagtgccg acattgccaa tatggggata agtaagagta acgatttgac
     6421 gagaatagga aggatcagtg aggatttctt gataaccggt cattgaagta ttgaaaacga
     6481 cttccccaac cgccgaacct gttgccccta tggcccgacc gtgaaactgg gttccgtctt
     6541 ccagaaccaa tagcgctgac ttaatcaaaa caccctccag agaatattca ctcactttat
     6601 ttgcatatta attcacaatg atgacgtaaa tcaatgcaaa tctgcttacc gacgaatttc
     6661 tggcaaacgg cggcattctg gagatatggg agctaaaagt caacttaatg gcgggatttt
     6721 ttatattatt ttgtggctca tgcatgtttt acaggcatta agcgacaaaa agatcatata
     6781 acatgttctg gaaaacgctt gcgcagcaag aagattgcat gaaatcaaaa tgagcagaaa
     6841 aaagtggacc aaacggtcaa aatttacatt aaaacaacaa aaacagaata caaaacatgt
     6901 agttttcagc acttgcatta aatttaaaag cataacacca aaaataaaag ggctaaaaaa
     6961 taaagccctt tatattcaat gcattaaatg tgttattttt gcatcactat aaatattttg
     7021 tggttacaga ttattgagat caagcacatc tcgcatatca aaaagaccac tttccttacc
     7081 actcaaccac aacgccgatc ttaccgcacc gttagcaaat gtcatgcggc tggatgcctt
     7141 atgggtgatc tccagacgct cgccaatatc ggcaaacatc gcggtatgtt caccaacgat
     7201 gtcacctgca cgcacggtgg caaaaccaat ggtgccaggc acacgttcac cggtatggcc
     7261 ttcacgactg tagaccgcgc aatctttcag atctttatca agagcgtggg cgatcgcctc
     7321 tcccattgcc agtgcggtgc ctgacggcgc atcaacttta tgtctatgat gtgcttcaat
     7381 aatttcgata tcggtgtagt cccccatcac tttggctgct ttctccagca gtttaagcat
     7441 gacgttaacg ccaacgctaa aattggcggc aaagacaatc gcaatatcgg cggctgcgtc
     7501 acgaatcgcc tgtttaccgg cttcgtcaaa ccccgtagtg ccgatcacca tccctttgcc
     7561 atgctggcga caaaaagcga gatgattcag cgtaccttcc ggacgggtaa aatcgataaa
     7621 cacatcaaaa tcatctttta ccgcatcgag gctgctttgc acggtaacgc ctgttttccc
     7681 tgctccagcc agctcaccgg cgtcgctgcc cagtaaagaa gatccttcgc gctccagcgc
     7741 cgcgcccagc tgcacgcctt ctaatgccag cgccgcctga atcaactggc ggcccatacg
     7801 ccccccggct cccgcgatgg caacgcggat gtttgcatca tgcatagcta ttctcttttg
     7861 ttaatttgca tagaccgttt tcagagtaac cagcgcatcg ctacgccgcc agccaaaaac
     7921 gctgataatt agaaattaat gataaacagg gagggatatc agtgaaaggc atgagtcgat
     7981 gaatgactgc atgccgataa catgtgacag gttacggcac cagagccagc acctcagcta
     8041 cccactgctg gaagcctttt acattcagat ccaatgccac ctgtacattg gctggtttgc
     8101 ccaggcaacc gtcgatatca accaccgtcg tgccagaggt aaattcgccc tgcgtttcca
     8161 ctgccacaaa acacggcttg agagtgaaca ggtccgggcg caccagccag gcgatggcgc
     8221 agagatcgtg cattcgcagt ccactttgca tactgccgct acggtagtga ctaaacaggg
     8281 cgtggagcat ttttccggtg cgattcaact ccggcaaagt agcgagatag tcaggggtta
     8341 atattgcctg attggtgaca tccagaccgc acatgacgat ttcgatgccg ctacggaaga
     8401 cacaggctgc cgcttctgga tcggcagcga tattaaactc ggcgtttggt gtgcagttgc
     8461 cgcgaccagc agaaccgccc atgatcacca gacgacgaat atacggttta cattccgggc
     8521 attgtgaaag taacagcgca atattggtta acgggccgat ggccaccagg gtaacaggct
     8581 caggcgcacg catcagggca tcacgaatcg ccagaaacgc tggtatccca agtggctttc
     8641 ggttgtgctc aacaaagtcg tagccagcca ttcccgattc gccgtgcaca gatgccgcat
     8701 cacgcggtgc gcgtaccagc ggcacagcgg ctccctgagc gagcggaatc tccgcattcc
     8761 agaaatgcag cagttgcagg gcgttgcggg tggttttctc aaccgagaca ttacccgcga
     8821 cggtggtcat cagttgcagg tcgagttcgg gggcaaaaat cgcggcagca atggcgacgg
     8881 catcgtcaat gccggggtca gtatcgagga agataggtaa acgcatgttt tctccataaa
     8941 aaatgccggt aacaagaccg gcattttcgc ataacttagg ctgctaatga cttaatcgac
     9001 ttcacgaata tcgacacgca gctctttcgg cacttcgaaa acaatgtttt cttcacggcc
     9061 ttccagcgga atggcttcac caccgcccag ctgctgcaaa cgtgccacca cattctgcac
     9121 cagaatatcc ggagccgatg cgcccgcagt cacgccgacg catttaacct ctttcaccca
     9181 ctcttcctgg atatctttcg catcgtcaat caaaaacgcg cgtttgccca tccgctgagc
     9241 cagctccgcc agacggttgg agttagagga gtttttcgaa ccgaccacca gcaccacttc
     9301 cgcctgctct gccagggcgc gtactgcttc ctgacggtta gtagtggcgt agcagatgtc
     9361 atctttgcgc ggaccgacaa ttttcgggaa gcgtttacgc agcgcgtcga tcacatcaga
     9421 cgtgtcatcc accgacagcg tggtttgggt cataaaggag agcttctctt cgtttttgac
     9481 cgtcagtttc cacacatcgt ctggcgattc gaccagatac attccccctt ccgggttgct
     9541 gtactgaccc atcgtccctt ccacttccgg gtgcccggcg tgaccgataa gaatagattc
     9601 ttcgccacga cggctggcgc gggcgacttc catatgcact ttggtcacca gcggacaggt
     9661 ggcatcaaac accgtcaaat cgcggctttt tgcttcgtta cgtacggcct gagaaacacc
     9721 gtgtgcggag aaaatcagga ttgcgccgtc cggcacttcg ctaatctgct caataaagat
     9781 agccccacgc tcacgcaagc tatcgaccac gtagcggtta tgcaccactt cgtgacggac
     9841 atatatcggt gcgccgtaaa tggccagcgc gttttcaaca atgctgatag cgcggtctac
     9901 cccggcacaa aaaccacgcg ggttggccaa caggatctgc atgttacgcc tccagtgccg
     9961 gatcgatttc cagcacttca atatcaaaat gaacggtctg cccggccagc ggatggttga
    10021 aatcaacggt aatggagtcg ccgttaattt cgcggatcac gccaggcatc tcactgccat
    10081 ccattgcggt aaaaagcatg attgcgccaa tttctggctc gcctgcatcc ataaactcac
    10141 ggcgggagaa gtactgaatc agatccggtg acggcacgcc aaacgccgca tctggctcca
    10201 gcgagaaagt ggttttatcg cccactttca gccccagcag gtgttgctcc agcccttcgg
    10261 aaagagaagc atcgcccagg cggaatagcg ccggtttacc gttgttgcgg gtagactcgg
    10321 cggtggtgcc atcgtcgagt tttagcgtga aatgcaccag gacggcgcta ttgctctgta
    10381 cagattcaga catgcaggtt tgctcttata ttttgaggat ttttgccgga tgctgcctac
    10441 gatttcgtag caatctgtag gccgcatccg gcagggttta ttattgtttt ttcgctttag
    10501 aaggtaaaaa cccttccagc acgatcagtg ccgcaccgac acaaatggca gtatcggcaa
    10561 ggttgaaggt ggcgaagtgc cagtcgccga cgtagaagtc gatcatatcg acaacgaagc
    10621 cgtgccacag gcggtcgaac aggttgccca gcgcgccgcc aataatcagc gcgtaagcga
    10681 tattgtttag cttctgcgtg gccttcgagc gatacatcat cactgccagg atcacgctaa
    10741 taccaatcgc aataccggca aagaaccaac gctgccagcc gccgctatcg gcaaggaaac
    10801 taaacgccgc gccatagtta cgcgcataat gcagattaag cgacgggaac agcgggaccg
    10861 tatcccccag agcaaagttc tggaggatca ggtacttgct gcccagatcg ataatcagca
    10921 cgactaccac cagccacagc cagcgtagcc ctgttgaaca gatcgattga ctcatcaggc
    10981 aaacttacgt ttttcaccgt caccggcgac gttgctgaca cagcggccgc agatttctgc
    11041 gtgttccgct accttgccga catcctgggt gtagtgccag cagcgtgggc acttctcacc
    11101 ttcggcttta ctcaacgcga ctttcagccc tttgagtact tcgctctgct gggcatcagc
    11161 aggtgcgtcg ttatagtctg caacggtagc gccggaggtc aacaggacaa atcgtaattc
    11221 atcgcccagc gcggtcagtt tcgccgccag ttccggttct gcgaacaagg tcactgccgc
    11281 ttccagcgag ccacccactt tcttgtcggc acgcgcttgc tcaatgactt tgttcacttc
    11341 gccacgcact ttcaacagct cgtcccagaa cgcatcgttc atcgcttcac tgtctgccag
    11401 accaaacagg ccttcgtacc actcgccggt gaagacgtat ttttcacgtt cacccggcag
    11461 gtagccccac acttcatcag cggtgaagga gaggattggt gccatccagc gcaccagcgc
    11521 ttccgcgatg tgatacagcg ctgtctggca gctacgacgc gccacactgt ccgctttggc
    11581 ggtgtactga cggtctttga tgatgtcgag gtagaaggaa cccatctcaa cggagcagaa
    11641 gcgcatcaga cgctgtacca cttcgtggaa atcgtatgct tcgtacgcct tgaggatgtc
    11701 ttcctgtgcc gctttcgcac aacctacggc ccagcgatcc agtaccacca tctcttccgg
    11761 tttcaccata tcttttgccg gatcaaaacc gttcaggttt gccagcagga agcgcgcggt
    11821 gttacggata cgacgatagc tgtcggcagc acgtttcagg atctcgtcag aaacggccat
    11881 ttcaccggtg tagtcagttg atgccaccca cagacgcaga atatccgcgc ccagtttgtt
    11941 catcacatcc tgcggcgaaa cggtgttgcc gatggattta gacatcttgc ggccctgacc
    12001 atccacggta aagccgtggg tcagtacctg acgatacggt gctttgccct tcatcgcggt
    12061 agagatcatc agagaggaca tgaaccagcc acggtgctgg tctgaacctt ccagatacat
    12121 gtccgcagcg tgaccggcaa attccggacg cacgtcaaca acggaagagt gggtagatcc
    12181 ggagtcaaac catacatcca gcgtatccgg cactttcacg tactgatcag cttcgtcgcc
    12241 gaggagctct ttcgcatcga gatcccacca cgcctggatg ccgtcaactt caacgcgttt
    12301 agccacttct tccatcagtt cgagggtacg cggatgcagc tcttccgtgt ctttatgcac
    12361 gaacagtgac atcggtacgc cccaggtgcg ctgacgggag atacaccagt caggacggtt
    12421 agcaaccatc gactcgatac gcgcctggcc ccagtccggg atccactgca cgcctttgat
    12481 ctctttcagt gactgcgcac gcagaccttt ctgatccatg ctgacgaacc actgcggcgt
    12541 cgcgcggaag atgatcggcg ttttgtgacg ccagcagcac ggatagctgt gctgcatttt
    12601 ctcaacgtgc aacagcgcgc ctttttcctg cagcagcgca acaacgatgt cgttcgcttt
    12661 gaaaacgttc acgccatcca gcgtcgggta agtacccggc agataagtgc cgtccgggcc
    12721 aaccgggtta gcggtttcca ggccgtattt ctgaccgatc acatagtcgt ccgggccgtg
    12781 gccaggcgcg gtgtgaacgg caccggtacc ggcatccagg gtaacgtgat cgccgaggat
    12841 tgccggaacg tcgaagccca taaacggatg ggtaaagcgc agcagctcaa gctccgcacc
    12901 ttttaccgtg ccgagaatgg tgtaatcggt cacgccgata cgctgcatta cgctttcaac
    12961 cagatctttc gccagaatca cggcctgacc gtcgatctgc accagcgcat agtcgaaatc
    13021 aggtgcaata gagattgcgc ggttcgcagg cagagtccac ggcgtagtgg tccagatcac
    13081 cagcgagatt gggccgttaa cgttgcttac ggcaaatttt gctttcagcg catcctgatc
    13141 gaccgcctgg aaagcaacgt cgatggacgg agaagttttg tcgtaatact caacttccgc
    13201 ttccgccagc gcagaacggc agtcaacgca ccagtgtacc ggcttcgcgc ctttgtgcag
    13261 gtgaccgttg ccgatgattt tgcccagcgc gcggatgata ttggcttcag ttttgaagtc
    13321 catggtcagg tacgggtggg accagtcacc cagcacgccc agacggataa agtctttgcg
    13381 ctgaccgtca acctgggtcg cagcgtattc gcggcacttg gcgcggaact cagcggcggt
    13441 gaatttctcc cccggcttac cgtattcttg ctctactttc agttcgattg gcagaccatg
    13501 acagtcccag ccaggcacat acggcgagtc ataaccggaa agccctttgg acttcacgat
    13561 aatgtctttc agaatcttgt taaccgagtg accaatatga atgctgccat tcgcataagg
    13621 agggccatca tgcagaatga aggttttttt gcctttttta gccgcacgga tgatgccgta
    13681 cagatcatca tcagtccaac gcgccagcat tcccggttcg cgcttggcga gatcgccacg
    13741 catcgggaac cctgtttccg gcaaattcag ggttgattta tagtcactca tcagattctc
    13801 ggttccgtgt ttcggtttga ttacataaca ggcttaagcc ggttttgtta gcccaaaaaa
    13861 ttcgcgggcg gttaattcat cacgcgcaat ctgcgctttc agttcgtcca gcgacgcaaa
    13921 tcgctgctca ttgcgtattt ttttacgcag cactacttgt atatggcgac cgtaaaggtc
    13981 cattgcaaca tctaacaaat gcacttccag ctgctggcga ataccggcaa ccgttgggcg
    14041 tgttccgatg tttgccacgc cgggtaacgg cttttcaccg aggcccagca cttctaccgc
    14101 ataaacccct ttcaccgggg aaacctgacg gcgcagcggt acattcgccg tcgggaaacc
    14161 tatagtgcgc cctaattcat caccgtggac tacacgcccg gagatggcaa acgggtgccc
    14221 cagtaaactt tctgccagag ccagattgtc atccgcaagg gcctgacgca cggcggtgct
    14281 gctgatacgc accccacctt cgcaaaaagt ttgcgtactg gtgatatcga agccgtattc
    14341 catgccagct ttctgtaata acaagaaatc gccttcacga ccagcgccaa agcggaaatc
    14401 atcacctacg gcaagaaatt ttacgcgcaa atgcttcacc agaagatcgc tgatgaaatt
    14461 ttgcgcggtt aacgccgcga aacgcctgtc gaaacgtacg cacagcacgt aatcaacgcc
    14521 acactctgca aggtaacgca gtttttcccg cagccgggtc agccgtgccg gggctttatc
    14581 ggttgcaaac agttccagtg gttgaggttc aaaaagcatc accatcaccg gtaagttgcg
    14641 cttgcgccct tcttcctgca agccctgtaa cagcgcgcga tgaccgcgat gcacgccgtc
    14701 gaaattacca atagtcagca cacacccttc ttgcggggcc tggctgagat tatgtatgcc
    14761 gcgtatcagc ttcatgtctg gctcaaaaca gtgaaaatcg tccgagtata ccttgtacag
    14821 cggtcaaggt taaccggcga ttgagtaccg aaatcagaaa gaagtggctt ttcattgcca
    14881 tggcgcaaat cacgggaaga aactgaccgc ctgctgcaat ttttatcgcg gaaaagctgt
    14941 attcacaccc cgcaagctgg tagaatcctg cgccatcact acgtaacgag tgccggcaca
    15001 ttaacggcgc ttatttgcac aaatccattg acaaaagaag gctaaaaggg catattcctc
    15061 ggcctttgaa ttgtccatat agaacacatt tgggagttgg accttggcta atatcaaatc
    15121 agctaagaag cgcgccattc agtctgaaaa ggctcgtaag cacaacgcaa gccgtcgctc
    15181 tatgatgcgt actttcatca agaaagtata cgcagctatc gaagctggcg acaaagctgc
    15241 tgcacagaaa gcatttaacg aaatgcaacc gatcgtggac cgtcaggctg ctaaaggtct
    15301 gatccacaaa aacaaagctg cacgtcataa ggctaacctg actgcacaga tcaacaaact
    15361 ggcttaatcg ccaatttgct gaagctttgt gaaaaagccc gcgcaagcgg gtttttttat
    15421 gcctgctgct tttgtgatgc gtgagaaaac acactgccgg ttgccagata gtcaaaagtt
    15481 acaggttctt gcagacatgc agttacactc tctgtactct tcgtgactta agaatcgtag
    15541 agtcatcggc tgatatattc tgaataagaa tatctactca atcttcagac tccccctaca
    15601 cagaatgtgg agattgtgat acacat
//
LOCUS       DACBVI010000105        15522 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.105, whole genome shotgun sequence.
ACCESSION   DACBVI010000105 DACBVI010000000
VERSION     DACBVI010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.105"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            478..2406
                     /gene="thrS"
                     /locus_tag="IU325_15995"
     CDS             478..2406
                     /gene="thrS"
                     /locus_tag="IU325_15995"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP5810502.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2410..2952
                     /gene="infC"
                     /locus_tag="IU325_16000"
     CDS             2410..2952
                     /gene="infC"
                     /locus_tag="IU325_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP5810503.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3049..3246
                     /gene="rpmI"
                     /locus_tag="IU325_16005"
     CDS             3049..3246
                     /gene="rpmI"
                     /locus_tag="IU325_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP5810504.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3300..3656
                     /gene="rplT"
                     /locus_tag="IU325_16010"
     CDS             3300..3656
                     /gene="rplT"
                     /locus_tag="IU325_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016169860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP5810505.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    3773..3897
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3779..3823
                     /locus_tag="IU325_16015"
     CDS             3779..3823
                     /locus_tag="IU325_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl--tRNA ligase operon leader peptide"
                     /protein_id="HAP5810506.1"
                     /translation="MNAAIFRFFFYFST"
     gene            3962..4945
                     /gene="pheS"
                     /locus_tag="IU325_16020"
     CDS             3962..4945
                     /gene="pheS"
                     /locus_tag="IU325_16020"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP5810507.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGNWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            4960..7347
                     /gene="pheT"
                     /locus_tag="IU325_16025"
     CDS             4960..7347
                     /gene="pheT"
                     /locus_tag="IU325_16025"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP5810508.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7352..7651
                     /gene="ihfA"
                     /locus_tag="IU325_16030"
     CDS             7352..7651
                     /gene="ihfA"
                     /locus_tag="IU325_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HAP5810509.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7752..8732
                     /gene="btuC"
                     /locus_tag="IU325_16035"
     CDS             7752..8732
                     /gene="btuC"
                     /locus_tag="IU325_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HAP5810510.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWS
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            8795..9346
                     /locus_tag="IU325_16040"
     CDS             8795..9346
                     /locus_tag="IU325_16040"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP5810511.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYLDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9346..10095
                     /gene="btuD"
                     /locus_tag="IU325_16045"
     CDS             9346..10095
                     /gene="btuD"
                     /locus_tag="IU325_16045"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HAP5810512.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10173..10637
                     /locus_tag="IU325_16050"
     CDS             10173..10637
                     /locus_tag="IU325_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP5810513.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            10884..11597
                     /gene="ydiV"
                     /locus_tag="IU325_16055"
     CDS             10884..11597
                     /gene="ydiV"
                     /locus_tag="IU325_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HAP5810514.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLDEGKDNRDLFSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEIMAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            11660..13096
                     /locus_tag="IU325_16060"
     CDS             11660..13096
                     /locus_tag="IU325_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HAP5810515.1"
                     /translation="MTLSFITRWRDELPATYTTLSPTPLNNARLIWHNAELANTLGIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQRLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVATSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLADDEDKYRLW
                     FTDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQYSAASPLRDEFIDRAAFDDW
                     FAHYRVRLQQDEVTDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13100..13291)
                     /gene="hemP"
                     /locus_tag="IU325_16065"
     CDS             complement(13100..13291)
                     /gene="hemP"
                     /locus_tag="IU325_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HAP5810516.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13422..14468)
                     /gene="aroH"
                     /locus_tag="IU325_16070"
     CDS             complement(13422..14468)
                     /gene="aroH"
                     /locus_tag="IU325_16070"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HAP5810517.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDSRF"
     gene            complement(14625..15458)
                     /gene="ppsR"
                     /locus_tag="IU325_16075"
     CDS             complement(14625..15458)
                     /gene="ppsR"
                     /locus_tag="IU325_16075"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HAP5810518.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT         3863 a         3686 c         4134 g         3839 t
ORIGIN      
        1 taattattat cgccagaacg aaatgtgcac gaaaaattgc tataagaatt gtgtcatttt
       61 tatcattgtg atatgaccac ttcgtcatta atcttatgat cgtttaaata gtacctccaa
      121 atcacgcaac gaactttgct ggattcagtt tatgctgctg caaatccgct cgcagtaaac
      181 ctttcagacg ctccgtgatg ttatcagtgg ttctttaagc gttttgctgg tgtactcact
      241 acaaacgaat tgcgaatcga atcaatgtga aacggaaagg tacaatctcc ctttttttag
      301 tgttaacatc gctcaaccgg gtggaaggtg agaggttaat gtctttcatt tgtgtacctg
      361 attgcgaacc aatttagcat ttgttggcta gatggtttcg caatgaactg ttaataaaca
      421 aatttttctt tgtatgtgat ctttcgtgtg ggtcaccact gcaaataagg atataaaatg
      481 cctgttataa ctcttcctga tggcagccaa cgccattacg atcacgctgt aagccccatg
      541 gatgttgcgc tggacattgg tccaggtctg gcgaaagcct gtatcgcagg gcgcgttaat
      601 ggcgaactgg ttgatgcttg cgatctgatt gaaaacgacg cacaactgtc gatcattacc
      661 gcgaaagacg aagaaggtct ggagatcatt cgtcactcct gtgcgcacct gttagggcac
      721 gcgattaaac aactttggcc gcataccaaa atggcaatcg gcccggttat tgacaacggt
      781 ttttattacg acgttgatct tgaccgcacg ttaacccagg aagatgtcga agcactcgag
      841 aagcggatgc atgagcttgc tgagaaaaac tacgacgtca ttaagaagaa agtcagctgg
      901 cacgaagcgc gtgaaacttt cgccaaccgt ggggagagct acaaagtctc cattcttgac
      961 gaaaacatcg cccatgatga caagccaggt ctgtacttcc atgaagaata tgtcgatatg
     1021 tgccgcggtc cgcacgtacc gaacatgcgt ttctgccatc atttcaaact aatgaaaacg
     1081 gcaggggctt actggcgtgg cgacagcaac aacaaaatgt tgcaacgtat ttacggtacg
     1141 gcgtgggcag acaaaaaagc gcttaacgct tacctgcagc gcctggaaga agccgcgaaa
     1201 cgcgaccacc gtaaaatcgg taaacagctc gacctgtacc atatgcagga agaagcaccg
     1261 ggtatggtat tctggcacaa cgatggctgg accatcttcc gtgaactgga agtgtttgtt
     1321 cgttctaaac tgaaagagta ccagtatcag gaagttaaag gtccgttcat gatggaccgt
     1381 gtcctgtggg aaaaaaccgg tcactgggac aactacaaag atgcaatgtt caccacgtct
     1441 tctgagaacc gtgaatactg cattaagccg atgaactgcc cgggtcacgt acaaattttc
     1501 aaccaggggc tgaagtcgta tcgcgatttg ccgctgcgta tggccgagtt tggtagctgc
     1561 caccgtaacg agccgtcagg ttcgctgcat ggcctgatgc gtgtacgtgg ttttacccag
     1621 gatgacgcgc atatcttctg tactgaagaa caaattcgcg atgaagttaa cggatgtatc
     1681 cgtttagtct atgatatgta cagcactttt ggcttcgaga agatcgtcgt caaactctcc
     1741 actcgtcctg aaaaacgtat tggcagcgac gaaatgtggg atcgtgctga ggcggacctg
     1801 gcggttgcgc tggaagaaaa caacatcccg tttgaatatc aactgggtga aggcgctttc
     1861 tacggtccga aaattgaatt taccctgtat gactgcctcg atcgtgcatg gcagtgcggt
     1921 acagtacagc tggacttctc cttgccgtct cgtctgagcg cctcctatgt gggcgaagac
     1981 aacgagcgta aggtaccggt aatgattcac cgcgcaattc tggggtcgat ggaacgtttc
     2041 atcggtatcc tgaccgaaga attcgctggt ttcttcccga cctggcttgc gccggttcag
     2101 gttgttatca tgaatattac cgattcacag tctgaatacg ttaacgaatt gacgcaaaaa
     2161 ctatcaaatg cgggcattcg tgttaaagca gacttgagaa atgagaagat tggctttaaa
     2221 atccgcgagc acactttgcg tcgcgtcccg tatatgctgg tctgtggtga taaagaggtg
     2281 gaatcaggca aagttgccgt tcgcacccgc cgtggtaaag acctgggaag catggacgta
     2341 aatgaagtga tcgagaagct gcaacaagag attcgcagcc gcagtcttaa acaattggag
     2401 gaataaggta ttaaaggcgg aaaacgagtt caaacggcgc gccctaaccg tatcaatggc
     2461 gaaattcgcg cccaggaagt tcgcttaaca ggtctggaag gcgagcagct tggtattgtg
     2521 agtctgagag aagctctgga gaaagcagaa gaagccggag tagacttagt cgagatcagc
     2581 cctaacgccg agccgccggt ttgtcgtata atggattacg gcaaattcct ctatgaaaag
     2641 agcaagtctt ctaaggaaca gaagaaaaag caaaaagtta tccaggttaa ggaaattaaa
     2701 ttccgtcctg gtacagatga aggcgactat caggtaaaac tccgcagcct gattcgcttt
     2761 ctcgaagagg gtgataaagc caaaatcacg ctgcgtttcc gcggtcgtga gatggcgcac
     2821 cagcaaatcg gtatggaagt gcttaatcgc gtgaaagacg atttgcaaga actggcagtg
     2881 gtcgaatcct tcccaacgaa gatcgaaggc cgccagatga tcatggtgct cgctcctaag
     2941 aagaaacagt aaggccttca agtagcactg tctgtggagc ctttgggctt cacaggcatt
     3001 gttcgccttt gtttcgttta ttaacaatgc gaagtggaag ttattaaaat gccaaaaatt
     3061 aagaccgtac gcggtgctgc taagcgcttc aaaaaaaccg gtaaaggtgg ttttaagcac
     3121 aagcacgcta acctgcgtca cattctgacc aaaaaagcga ccaaacgtaa acgtcacctg
     3181 cgtccgaaag ccatggtttc caaaggcgat ctgggcctgg taatcgcgtg cctgccgtac
     3241 gcataagccg ttaacgtttt ttaacttttt aattagaata tagatacagg agagcacata
     3301 tggctcgcgt aaaacgtggt gttattgcac gcgcacgtca caagaaaatt ttgaaacaag
     3361 ctaaaggcta ctacggtgcg cgttctcgcg tataccgcgt tgccttccag gctgttatca
     3421 aagctggtca gtatgcttac cgtgaccgtc gtcaacgtaa gcgtcagttc cgtcaactgt
     3481 ggattgcgcg tatcaacgca gcagcacgtc agaacggtat ttcttacagc aaattcatca
     3541 atggcctgaa aaaagcctct gttgaaatcg accgtaagat cctggctgat atcgcagtat
     3601 tcgacaaagt agcgttcacc gctctggttg aaaaagcgaa agcagctctg gcataagcca
     3661 gttgaaagag ggagctagtc tccctctttt cgtttcaacg ccatcaaaac attgactttt
     3721 atcgccgtag ccttttcaat aaaggtcttt tgaaaagtac caaaaggtaa cgcaagcaat
     3781 gaatgctgct attttccgct tcttttttta ctttagcacc tgactccagg aggctagcgc
     3841 gtgagaagag aaacggaaaa cagcgcctga aagcctccca gtggaggctt tttttgtatg
     3901 cgcgttttga aaaaattaag tccaacgaac cagtgtcacc actgacacaa tgaggaaaac
     3961 catgtcacat ctcgcagaac tggttgccag tgcgaaggcg gccattagcc aggcgtcaga
     4021 tgttgccgcg ttagacaatg tgcgcgtcga atatttgggt aaaaaagggc acttaaccct
     4081 tcagatgacg accctgcgtg agctgccgcc agaagaacgc ccggcagccg gtgcggttat
     4141 caacgaagcg aaagagcagg ttcagcaggc gctgaatgca cgtaaagcgg aactggaaag
     4201 cgcagcgctg aatgcgcgtc tggcggcgga aacgattgat gtctccctgc cggggcgtcg
     4261 tattgaaaac ggcggtctgc atccggtgac ccgtaccatc gaccgtatcg aaagtttctt
     4321 cggtgagctt ggctttaccg tggcaaccgg gccggaaatc gaagacgatt atcataactt
     4381 cgatgctctg aacattcctg gtcaccaccc ggcgcgcgct gaccacgaca ctttctggtt
     4441 tgacgctacc cgcctgctgc gtacccagac ctctggcgta cagatccgca ccatgaaagc
     4501 tcagcagcca ccgattcgta tcatcgcgcc tggccgcgtt tatcgtaacg actacgacca
     4561 gactcacacg ccgatgttcc atcagatgga aggtctgatt gttgatacca acatcagctt
     4621 taccaacctg aaaggcacgc tgcacgactt cctgcgtaac ttctttgagg aagatttgca
     4681 gatccgcttc cgtccttcct acttcccgtt taccgaacct tctgcagaag tggacgtcat
     4741 gggtaaaaac ggtaattggc tggaagtact gggctgcggg atggtgcatc cgaacgtgct
     4801 gcgtaatgtt ggcatcgacc cggaagttta ctctggtttc gccttcggaa tggggatgga
     4861 gcgtctgacc atgttgcgtt acggcgtcac cgacctgcgt tcattcttcg aaaacgatct
     4921 gcgtttcctc aaacagttta aataaggcag gaatagatta tgaaattcag tgaactgtgg
     4981 ttacgcgaat gggtgaaccc ggcgattgat agcgatgcgc tggcaaatca aatcactatg
     5041 gcgggcctgg aagttgacgg tgtagaaccg gttgccggta gcttccacgg cgtggtcgtt
     5101 ggtgaagtgg ttgagtgtgc gcagcatccg aacgctgaca aactgcgtgt gacaaaagtg
     5161 aatgtcggcg gcgatcgcct gctggacatc gtctgcggtg cgccaaactg ccgtcagggc
     5221 ctgcgtgtgg cggtagcgac cattggtgct gttctgccgg gcgatttcaa aattaaagcg
     5281 gcgaaactgc gtggcgaacc gtctgaaggg atgctgtgct ccttttctga gctgggaatt
     5341 tctgacgatc acagcggcat tatcgagctg cctgcagatg cgccgattgg cacagacatc
     5401 cgcgaatacc tgaaactcga tgacaacacc atcgaaatca gcgtaacgcc aaaccgtgcc
     5461 gattgcttag gcattatcgg cgtggcgcgt gatgttgccg tgctgaacca gctgccgctg
     5521 gttgaaccgg aaatcgttcc ggttggtgcg accatcgacg acacgctgcc gattacagtc
     5581 gaagcgccgg aagcctgccc gcgctatctt ggccgtgtgg taaaaggcat taacgttaaa
     5641 gcgccaactc cgctgtggat gaaagaaaaa ctgcgtcgtt gcgggatccg atctatcgat
     5701 gcagtcgttg acgtcaccaa ctatgtactg cttgaactgg gccagccgat gcacgctttc
     5761 gataaagatc gcattgaagg cggcattgtg gtgcggatgg cgaaagaggg cgaaacgctg
     5821 gtgctgcttg atggcactga agcgaagctg aatgctgaca cccttgtcat cgccgaccac
     5881 aacaaggcgc tggcgatggg cggcatcttt ggtggcgaac actctggcgt gaatgacgaa
     5941 acgcaaaacg tcctgctgga atgcgctttc ttcagcccgc tgtctatcac cggtcgtgct
     6001 cgtcgtcatg gcctgcatac cgatgcgtct caccgttatg agcgtggcgt tgatccggca
     6061 ctgcagcaca aagcgatgga acgtgcgacc cgtctgctga tcgacatctg cggcggtgag
     6121 gctggcccgg taattgatat caccaacgaa gcaacgctgc cgaagcgagc caccattact
     6181 ttacgtcgca gcaaactgga tcgcctgatc ggccatcata ttgccgatga gcaggtaact
     6241 gatattctgc gtcgtctcgg ctgcgaagtg accgaaggca aagacgaatg gcaggcagtt
     6301 gcgccgagct ggcgtttcga catggagatt gaagaagatc tggtcgaaga agtcgcgcgt
     6361 gtttacggct acaacaacat ccctgatgag ccggtgcagg caagcctgat tatgggtact
     6421 caccgcgaag cagatctgtc gctcaagcgc gtgaaaacgc tgctcaacga caaaggctat
     6481 caggaagtga tcacctacag cttcgttgat ccgaaagtgc agcagatgat ccatccaggc
     6541 gttgaagcct tactgctgcc aagcccgatt tccgttgaaa tgtcagcaat gcgtctttcc
     6601 ctgtggaccg gcctgctggc aaccgtggtg tacaaccaga accgtcagca gaaccgtgtg
     6661 cgcattttcg aaagtggtct gcgtttcgta ccagatactc aggcaccgtt gggcattcgt
     6721 caggatctga tgttagccgg tgtgatttgc ggtaaccgtt acgaagagca ctggaacctg
     6781 gcaaaagaga ccgttgattt ctatgatttg aaaggcgatc tggaatccgt tctcgacctg
     6841 accggtaaac tgaatgaggt tgagttccgt gcagaagcga atccggcact gcatccgggg
     6901 caatccgcag cgatttatct gaaaggtgaa cgtattggtt ttgttggggt tgttcatcct
     6961 gaactggaac gtaaactgga tcttaacggt cgcactctgg tgttcgaact ggagtggaac
     7021 aagctcgcag accgcgtggt gcctcaggcg cgcgagattt ctcgcttccc ggcgaaccgt
     7081 cgtgacatcg cggtggtggt cgcagaaaac gttcccgcag cggatatttt atccgaatgt
     7141 aagaaagttg gcgtaaatca ggtagttggc gtaaacttat ttgacgtgta ccgcggtaag
     7201 ggtgttgcgg aggggtataa gagcctcgcc ataagcctga tcctgcaaga taccagccgt
     7261 acactcgaag aagaggagat tgccgctacc gtcgccaaat gtgtagaggc attaaaagag
     7321 cgattccagg catcattgag ggattgaacc tatggcgctt acaaaagctg aaatgtcaga
     7381 atatctgttt gataagcttg ggcttagcaa gcgggatgcc aaagaactgg ttgaactgtt
     7441 tttcgaagag atccgtcgcg ctctggaaaa cggcgaacag gtgaaactct ctggttttgg
     7501 taacttcgat ctgcgtgata agaatcaacg cccgggacgt aacccgaaaa cgggcgagga
     7561 tattcccatt acagcacggc gcgtggtgac cttcagaccc gggcagaagt taaaaagccg
     7621 ggtcgaaaac gcttcgccca aagacgagta atctgatcta actaaaaagg ccgctttgcg
     7681 gccttttttc ttttcactgt cgaagagtca ccgtaaaatc aacgccatga cacttcagca
     7741 gaacggatac catgctgaca cttgcccgcc aacaacagcg acaaaatatt cgctggttat
     7801 tatgcctgtc agttttgatg ctgctggcgc ttctcttaag cctttgcgcc ggtgaacaat
     7861 ggatttcgcc cggtgactgg tctactcctc gtggcgaact gttcgtctgg caaattcgcc
     7921 tgccacgtac gctggctgta ttgctggttg gtgcggcgct ggctatatcc ggcgcagtaa
     7981 tgcaggcgtt gtttgaaaat cctctggcag aacctggact acttggcgtc tctaacggcg
     8041 caggcgtagg gcttatcgcc gcggtgttgc ttgggcaagg gcaactccca aactgggcac
     8101 tagggctgtg tgcgattgct ggcgcgctta tcatcacttt aatactctta cgtttcgccc
     8161 gtcgtcatct ttcgaccagt cggttattgc tggctggcgt tgcattaggg attatctgta
     8221 gcgcactaat gacgtgggct atctactttt ccacctctgt tgatttacgt cagctgatgt
     8281 actggatgat gggcggtttt ggcggcgtag actggcggca aagctggctg atgctggcat
     8341 tgatccccgt gttgttgtgg atctgttgtc agtccaggcc gatgaatatg ttagcacttg
     8401 gcgagatctc ggcgcggcaa ctgggtttac ccctgtggtt ctggcgcaat gtgctggtgg
     8461 cagcgaccgg ctggatggtt ggcgtcagtg tggcgctggc gggtgctatc ggctttattg
     8521 gtctggttat cccacatatt ctccggctgt gtggtttaac cgatcatcgc gtattacttc
     8581 ccggctgcgc gctggcaggg gcgagcgcat tgctgctggc cgatattgta gcgcgcctgg
     8641 cattagctgc cgcagagctg cctattggcg tggtcaccgc aacgttgggt gcgccggtgt
     8701 ttatctggtt attgttaaaa gcaggacgtt agccgcaaaa agacggtcta tgattaaaag
     8761 ctaagatttt tactgaccac acccaggaga aacgatgcaa gattccattc tgacgaccgt
     8821 agtgaaagat atcgacggtg aagtgaccac gctggagaag tacgccggta atgtgctgtt
     8881 gattgtcaat gtcgcctcaa agtgtggctt aacgccgcaa tatgagcagt tggagaatat
     8941 tcagaaagcc tgggccgatc gtggttttgt ggtgctggga ttcccgtgca accagtttct
     9001 ggagcaagaa ccgggcagcg atgaagagat taaaacttac tgcaccacta catggggggt
     9061 gacgttcccg atgttcagta agattgaagt taatggcgaa ggacgccatc cgctgtatca
     9121 aaaattgatt gccgcagcgc cgaccgcagt cgcgccggaa gagagcggat tctatgcccg
     9181 tatggtcagc aaaggtcgtg caccgctgta ccttgatgat attttatgga attttgagaa
     9241 atttttagtt ggcagggacg gattggtcat ccagcgtttt tccccggata tgacgccgga
     9301 agatcccatt gtgatggaaa gcattaaact ggcgttggcg aaataatgtc tattgtgatg
     9361 cagttacagg atgttgcgga atctacccgc ctggggccgc tttctggcga ggttcgggct
     9421 ggggagatcc tgcatctggt ggggccgaac ggcgcgggta agagtacctt actggcgcga
     9481 atggccggaa tgaccagcgg taagggaagc attcagttcg cggggcaacc actggaagca
     9541 tggtccgcaa caaaactcgc gctgcatcgc gcctatcttt cacaacaaca gacgccaccg
     9601 tttgcaatgc cggtctggca ctacctgaca ctgcatcagc acgataaaac gcgtaccgaa
     9661 ctactgaatg atgtcgcagg ggcgctggct cttgatgaca aactcggacg tagcaccaat
     9721 caactttccg gcggcgaatg gcaacgcgta cgtcttgctg cggtggtgct gcaaatcaca
     9781 ccacaagcca atcccgcagg tcaattgctg cttcttgatg agccgatgaa cagtcttgat
     9841 gttgcgcaac aaagtgcgtt agacaaaatt ctgagcgcgc tgtgtcagca aggactggcg
     9901 attgtgatga gcagtcacga tctcaaccat acattgcgtc atgcgcatcg ggcatggttg
     9961 ctaaaaggtg gaaaaatgct ggccagtgga cgcagggaag aggtgctcac gccgccaaat
    10021 ctggcgcagg cctatgggat gaattttcgc cgtctggata tcgaaggtca cagaatgctg
    10081 atttcgacca tctgatttca ttagtatctt gaaaatcggg taattacgac gctaaattaa
    10141 tgcaagaata aaaaacagag gaaccgtacg gaatgcgttt ctgccttatt ttgatcacag
    10201 cactgtttct ggccgggtgt agccaccata aagcaccgcc gccaaatgcc agactttctg
    10261 attcgattac cgttattgcc ggtttgaacg accagctaca aagctggcat ggcacgccgt
    10321 atcgttatgg tggcatgacg cggcgcggtg tggactgttc gggatttgtg gttgtgacga
    10381 tgcgcgatcg tttcgatttg cagctgcccc gagaaaccaa agaacaagcc tctatcggca
    10441 cgcaaattga taaagacgag ttgctgcctg gtgacctggt ctttttcaaa acgggttccg
    10501 gacaaaatgg tttgcatgta ggtatttacg ataccaacaa ccaatttatc cacgcctcta
    10561 ccagcaaggg agtgatgcgt tcctcactcg ataatgtcta ttggcagaaa aatttctggc
    10621 aggcgagacg aatctagtat gtcgaaaaat ggacggcgat gatacatctc cgtccattta
    10681 ctcctgaata cagtagtatt ttacagacaa aaattccgta aatggattat tcgtttataa
    10741 tcaggataat ctaataaaag gacccctgaa aaagtggtta acatggaaca aatgaaagtt
    10801 actccttctg taattgttat gtcttatggc ctcatccctt ttagccggat actgaaaaac
    10861 atccttcgag agggacggtt accatgaaga tttttttgga gaatctttat cattctgatt
    10921 gctatttcct gccgataaga gataaccagc aagaccttgt cggcgttgag ttaattaccc
    10981 atttctccag tgaagatggt acggttcgta tcccgacgag tcgggttata gcgcaactta
    11041 ccgaggagca acattggcaa ttattttcag agcagttgga attactaaaa tcatgccaac
    11101 acttttttat tcaacataaa ctctttgctt ggcttaattt aacgccacaa gtggctactt
    11161 tattattaga cagggataat tacgcaggcg aactattaaa atatccgttt attgaactgt
    11221 tgattaatga gaattacccg catctcgatg aaggaaaaga taatcgcgat cttttttcct
    11281 tatcgcaaat gtatcccctt gtgttaggta atttgggcgc aggcaatagc acaatgaaag
    11341 cggtttttga tggtcttttc acccgcgtca tgctggataa aagttttatt cagcaacaaa
    11401 tcacgcatcg ctccttcgaa ccgtttattc gtgcgatcca ggcgcaaatt tccccatgtt
    11461 gcaattgcat catcgcgggg ggcattgata cgccagaaat aatggcacag attaccccct
    11521 ttgatttcca tgctctccag ggatgcctgt ggcctgccgt tcccataaat cagataacga
    11581 cgttggttca gcgataaccc tcctgtttgc tggtgtttaa gacgagagta accgtctaca
    11641 ctatcaaaca ggaggatcta tgaccctgtc ttttattacc cgttggcgcg atgaattgcc
    11701 agcaacctat acaacacttt cccctacgcc tttaaataat gcccggctga tttggcataa
    11761 tgccgaactg gctaacacgc tgggtattcc atcgtcactg tttaaaaatg gcgcaggtgt
    11821 ctggggtggc gaaaccctac tgcctggtat gtcaccactg gcgcaggttt acagtggtca
    11881 tcagttcggc gtctgggcgg gccaactggg tgatgggcgc ggcattttac tcggcgaaca
    11941 acggcttgct gatggcacta caatggactg gcatctgaaa ggtgctggcc tgacgcctta
    12001 ttcgcgaatg ggtgatggac gggcggtttt acgttcgacg atacgagaaa gtctcgccag
    12061 tgaggcgatg cattatctgg gcattccgac gacccgcgct ttaagtatcg tcaccagcga
    12121 ttcgccagtg tatcgggaaa cggtggagcc aggcgcgatg ctgatgcgtg tggcaacaag
    12181 tcatctgcgc tttggtcatt tcgaacattt ttactatcgc cgcgagccgg aaaaggttcg
    12241 tcagttagct gactttgcca ttcgtcatta ctggtcacat cttgcagatg atgaggacaa
    12301 ataccgtctc tggtttaccg atgttgtcgc acgtaccgca tcgttaattg cccaatggca
    12361 gacggtcggc tttgctcatg gggtgatgaa taccgacaac atgtcgctgt tggggctgac
    12421 gcttgattac gggccgtttg gttttcttga tgattacgaa cccggtttta tttgtaatca
    12481 ctcggatcac caagggcgtt acagctttga taatcaacct gccgtcgcgt tgtggaattt
    12541 acagcgtctg gcgcagacat tgtcaccatt tgtcgccgtt gatgccctga atgaggccct
    12601 ggatagctat cagcaggttt tgttgacgca ttatggacaa cggatgcggc agaaactggg
    12661 cttcatgacg gagcaaaaag aggataacgc gctactgaat gaattattca gtctgatggc
    12721 gcgagagcgc agcgattata cccgcacatt ccgcatgctg agtctgaccg agcagtacag
    12781 cgcggcgtca ccgctacgtg atgagtttat cgatcgtgcg gcatttgatg actggtttgc
    12841 ccattatcgg gtacgtttgc aacaagacga ggttaccgat agtgagcgtc agcagctgat
    12901 gcaaagcgtt aacccagctc tggtgttgcg caactggttg gcgcaacggg cgattgaagc
    12961 ggcagaaaag ggtgatatga cggaattgca ccgcctgcat gaggcgttgc gaaatccttt
    13021 cagcgacaga gatgatgact acgtcagtcg tccacctgac tggggtaaac ggctggaagt
    13081 cagctgctcg agttaaaggc tacttggtca acagcagctt gccagcctga gttttacgga
    13141 gcagatattc ttgcccgtca tgatcgataa tcagtttgcc atccggacct aacagtgtct
    13201 ggctggtaat tcgccggatg ggctgcgggg aagcggtctt gtgattggca tccgtttcag
    13261 gcgtgagttt tctgctatcc gtataacgca taaaaattaa taataattga tataacaatg
    13321 attcttatta tcaatattat ggcggccaga gcaagcagta atttgttgag aagacaacaa
    13381 gttgcgcaga aatgcccatt ccgtcaggaa tgggcacgca ttcagaagcg gctatctacc
    13441 gcagaggcga gtttttcgac caggcgttcg gtatcttccc agcccagaca cgggtcggta
    13501 atcgattgac cgtaagtgag cggctgaccg ccgacgattt tttgcgttcc ttcgcgcagg
    13561 aaactttccg ccataattcc agcaatcgcc gtagagccat tgcggatttg ctgacaaata
    13621 tcctcacaaa cttctaactg gcgacggtgc tgcttctggc agttaccgtg gctgaaatcc
    13681 accaccagat gttcaggtaa atcaaactcg tgcagcgtat cgcaggctgc ggcgatatca
    13741 tcggcatgat aattcggttt tttgccgcca cgcataataa tgtggccata cgggttgccg
    13801 ctggtctgat agatggtcat ctgaccgttt ttgtctggcg agaggaacat atggctggcg
    13861 cgggctgcgc ggatagcatc tacggcaatc cgcgtgttgc catcggtacc atttttaaaa
    13921 cctaccggac aggagagtgc cgaagccatt tcacggtgga tctgactttc ggtagtacgt
    13981 gcgccaatcg cgccccaact gattaaatca gcaataaact gaccggtcac catatcgagg
    14041 aactcagtcg cggtagggac gcccagctca tttacctgta aaagtaattt gcgcgccagc
    14101 tccagaccgt gatttacccg atagctgccg tttaaatctg gatcggagat tagccctttc
    14161 cagccgacaa cagttcgtgg tttttcaaaa taggtgcgca ttacgatttc cagccgtgac
    14221 tggtactggt tgcgcagcga ctgcagacgg gtggcgtact ccattgcagc ggtgagatcg
    14281 tggatcgagc aggggccaat aatgaccaac agtcgcttat cttcaccatt cagtattttt
    14341 tcaattctgc ggcgggagtc ggtgacatgg gtggcgacgc caggcgttac gggataccgt
    14401 agcgcgagtt cggcgggcgt taccaggctc tcaatacgcg cagtacggag ttcgtcagtt
    14461 ctgttcatta caagtctcag aaatttgttg cttctacggc aggactaccg ggaagtgatg
    14521 cgcatcacga taaaccaatc gcagctgatt tcaagtgcga ggtgtgtcac acctcgccgg
    14581 gtggttagca tgataacaaa aaaataagct aatgcactag ttctctagta cattcggcgg
    14641 ctaaggccca tgatatcgag gatcttggtg gcaatctctt ctaccgaata attggtactg
    14701 ttaatccacg ggatctgatt tttacggtac aaggcttcta cttccgcgac ttccatcctg
    14761 cactgacgaa gcgaggcata gcgactgttc tcccgacgtt cttcgcgaat cgccgccaga
    14821 cgttccgggt cgatagtcag gccgaacaat ttatgctgaa gcggtttgag cgacgcgggt
    14881 agcaccagat tatccatgtc gtcggcaata aaggggtagt ttgccgcgcg gataccaaat
    14941 tgcattgcca gatacagact ggtgggggtt ttaccacagc gagaaacacc gagcaggatc
    15001 acctgagcct gatccagatt gcgcaacgaa atgccgtcat cgtgggcgag ggtgtaatca
    15061 atcgccgcaa tgcgcgcatc atatttattg agattattag ggttaaggcc atgggtacga
    15121 tgagcaatcg gcgttggatc cagtttcatc tcttgttgta gcggggcaac cagcgcctga
    15181 acgatatcct ggcaaaagcc ttcactttgc aagatgatgg cgcgaatctc cggcaacacg
    15241 atggagtaga agaccagcgg gcgcacgcct gtctggtgat aaattgcgtc aatctgatcc
    15301 ttcactgccc gtgcacggct ctcattttcg acaaacggca gcgtaatgct gctgatagtg
    15361 acgggaaatt gtgacattac tgcgtgtcct aatacctccg cagttattgc cgtaccatca
    15421 gaaatataaa aaacgtggcg atcaacagca ttatccattt tgttcttccc gtgatgcaga
    15481 cataattcta tgaaagcata aattaaaaac gcacagaagc gt
//
LOCUS       DACBVI010000106        15396 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.106, whole genome shotgun sequence.
ACCESSION   DACBVI010000106 DACBVI010000000
VERSION     DACBVI010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.106"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            1..398
                     /locus_tag="IU325_16080"
                     /pseudo
     CDS             1..398
                     /locus_tag="IU325_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(503..1552)
                     /locus_tag="IU325_16085"
     CDS             complement(503..1552)
                     /locus_tag="IU325_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAP5810519.1"
                     /translation="MVIRGMTISLPWFAFINVSFALIILLRRVLFNDLTPPWLNEKSL
                     IHSIDISATGILLICSGLLLIPRQKTLPIQVLLVALSLLWSWCSYHFIAYWTLQFAYP
                     LCVLLMLSGVVALYFHTPSLLAFVIPLWFTTPIASLMLNQQINIHFAVVWCIFSLALY
                     GGRLILLRWFEEAWVQNSYNNQLINRLDALAHRDPLTGIANRRAMNSILHDAMDNGGS
                     FALIMLDVDFFKRYNDTYGHPAGDRCLIQVADALQRSTRQSEDVVVRYGGEEFAIILF
                     NATLPEAETVAARVKQELKLAAIPHQASAVNAFVTVSQGITCSAPAKTAEQIISDADA
                     ALYRAKESGRNRWAL"
     gene            complement(1647..3623)
                     /locus_tag="IU325_16090"
     CDS             complement(1647..3623)
                     /locus_tag="IU325_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP5810520.1"
                     /translation="MQLNHIVKSLLITGLFTTSSLPLLAAEAPKDATAATQQANNLLY
                     NQLPFADNTDFTDAHKGFIAPIPQDVIKGEQGNVIWDPQHYAFIKEGDKAPDTVNPSL
                     WRQAQLINISGLFEVTEGVYQIRNLDLSNMTIIEGKEGITVVDPLVSAETAKVGMDLY
                     YKNRGKKPVVAVIYTHSHVDHYGGVRGVIDEADVKSGKVKIYAPAGFLEEAVSENIMA
                     GNVMSRRASYMYGNLLKPDVKGQVGAGLGTTTSAGTVTLIAPTNYITKTGQIETIDGL
                     TYDFLMAPGSEAPSEMLWFIEEKKLIETAEDVTHTLHNTYSLRGAKIRQPLPWSKYIN
                     EALNLWGDKAEIILAQHHWPTWGNDNVVKLLKSQRDLYRYINDQTLRMANQGMTRDEI
                     AANFKLPSSLANTWANRGYYGSVSHDVKATYVLYLGWFDGNPATLDELPPEEGAKKFV
                     EYMGGADAILQKATQDYDQGNFRWVAQVVSKVVFADPNNQAARDLEADALEQLGYQAE
                     SGPWRNFYLTGAQELRNGVQKLPTPNTASPDTVRAMTPEMFFDYLAVHINGEKAADAK
                     TVLNFDFGEDGGTYKVELENGVLNHTAGVEASDADATITLSRDVLNKIVLKEETLKEA
                     TAKGDVKITGNEEKLNELLGYMDNFEFWFNIVTP"
     gene            3929..4156
                     /gene="pptA"
                     /locus_tag="IU325_16095"
     CDS             3929..4156
                     /gene="pptA"
                     /locus_tag="IU325_16095"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HAP5810521.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISISL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(4160..4729)
                     /locus_tag="IU325_16100"
     CDS             complement(4160..4729)
                     /locus_tag="IU325_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810522.1"
                     /translation="MSRFIPVELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGRDEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQEKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            4904..5749
                     /gene="nhoA"
                     /locus_tag="IU325_16105"
     CDS             4904..5749
                     /gene="nhoA"
                     /locus_tag="IU325_16105"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HAP5810523.1"
                     /translation="MTPILNHYFARINWLGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVTARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVEVEGEKWIADVGFGGQTLTAPIRLVPELVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHTVEQRNLPDVASLYAVMQEQFGLGVDDVKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            complement(5833..6726)
                     /locus_tag="IU325_16110"
     CDS             complement(5833..6726)
                     /locus_tag="IU325_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HAP5810524.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGPPGFEPPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLHVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEF"
     gene            complement(6805..7485)
                     /gene="narI"
                     /locus_tag="IU325_16115"
     CDS             complement(6805..7485)
                     /gene="narI"
                     /locus_tag="IU325_16115"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HAP5810525.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAVKQQMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMIILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            complement(7482..8177)
                     /gene="narW"
                     /locus_tag="IU325_16120"
     CDS             complement(7482..8177)
                     /gene="narW"
                     /locus_tag="IU325_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HAP5810526.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTRNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREASWYALFDTLLQLAGS
                     TLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            complement(8177..9721)
                     /gene="narH"
                     /locus_tag="IU325_16125"
     CDS             complement(8177..9721)
                     /gene="narH"
                     /locus_tag="IU325_16125"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAP5810527.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRAHDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            complement(9718..13458)
                     /locus_tag="IU325_16130"
     CDS             complement(9718..13458)
                     /locus_tag="IU325_16130"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAP5810528.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMVRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKNNPV
                     LVRQLPVKNLTLADGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMHYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKIS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     QELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKREGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            complement(13553..14941)
                     /locus_tag="IU325_16135"
     CDS             complement(13553..14941)
                     /locus_tag="IU325_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK family nitrate/nitrite MFS transporter"
                     /protein_id="HAP5810529.1"
                     /translation="MALQNEKTSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            complement(15256..>15396)
                     /locus_tag="IU325_16140"
     CDS             complement(15256..>15396)
                     /locus_tag="IU325_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAP5810530.1"
                     /translation="RNIKIKNMTLDEINNTYCNNDYYNEAIREEPIDFLDRTFSSSSW
                     PF"
BASE COUNT         3901 a         3868 c         3815 g         3812 t
ORIGIN      
        1 gaaggctggg aagatataga ggattttggg gaaacacatc tcgatttttt gaagcaatat
       61 ggtgattttg ataatggtat tcctgttcac gataccattg ccagagttgt atcctgtatc
      121 agtcctgaaa aatttcatga gtgctttatt aactggatgc ttgactgcca ttcttcagat
      181 gataaagacg tcattgcaat tgatggaaaa acgctccggt actcttatga caagagtcgt
      241 cgcaagggag cgattcacgt tattagtgtg ttctcaataa tgcacagtca gattccttgg
      301 aagatcaaga cgaatgaaaa aaacaatgag attacagctg tttctgaatt tcttaacata
      361 atgggtatta aaggaaaaat aattgcaccg atccaatgtg ataccagaaa gacatcgcta
      421 agaagatagc tatagacaat tattaccctc cgtcagccct tgttaagcga ggagagacgt
      481 aatctgtcca gtcccctaat gcttataaag cccatcgatt tcgcccactt tctttcgccc
      541 ggtataacgc agcgtcggca tctgaaataa tttgttcagc cgtttttgcg ggagcagaac
      601 aggtaatccc ctgactaacc gtaacaaatg cattaacagc agatgcctga tgtgggatag
      661 ctgccagttt aagttcttgc ttcactctgg ctgctaccgt ctctgcttcc ggcaatgttg
      721 cattaaaaag tatgatggca aactcttcgc caccataacg gacaacaaca tcctccgact
      781 ggcgagttga acgctgtaga gcgtcggcaa cttgtattag acaacgatcc ccagcaggat
      841 gcccgtaagt gtcgttgtaa cgtttaaaaa aatcgacatc cagcataata agagcaaatg
      901 acccgccgtt atccatcgcg tcgtgtaaga tactgttcat tgcacgtcgg tttgctatcc
      961 cagttaaagg atcacgatgt gctaatgcat caaggcgatt aattaactgg ttgttataac
     1021 tgttttgtac ccatgcttct tcaaaccaac gtaacaaaat gagtcgccca ccatatagcg
     1081 ccagtgaaaa aatacaccat acaacagcaa aatgaatatt gatctgttga ttaagcataa
     1141 gactcgctat tggagtggtg aaccataagg gaatgacaaa tgcgagcaat gatggggtat
     1201 ggaaataaag tgcgacaacg ccactaagca ttaataatac acatagtgga taagcaaact
     1261 gcagcgtcca gtacgcaata aagtgataac tacaccatga ccatagcagg ctcagggcaa
     1321 ctaatagcac ctggataggt aatgtttttt gccgagggat gagtagtaaa ccactgcaaa
     1381 tcagcagaat acccgttgca gatatatcaa tagaatgtat aaggcttttt tcatttaacc
     1441 agggtggagt aaggtcatta aacagaacac ggcgcagcaa aattatcaat gcaaaactga
     1501 cattgataaa agcaaaccat ggcaaactaa tcgtcatgcc gcgaataacc atatcgcggc
     1561 gggttttcca tgagtgatga gtttttcttc gttcagccat tttttaacca aaaaagggca
     1621 ggatacctgc ccggtatgca gtaaatttat ggtgtcacta tattgaacca aaattcaaaa
     1681 ttatccatat aacctaacag ctcgttgagt ttttcctcat tgccagtaat tttgacatct
     1741 cctttggccg tggcttcttt cagcgtctct tctttcagta caattttgtt caatacatca
     1801 cgagacagag tgatagtggc atcagcatcc gaagcctcta caccagcggt atgattgaga
     1861 acaccatttt caagctccac tttataggtg ccgccatctt caccaaaatc gaaattcaac
     1921 acggttttgg catctgccgc tttctcacca ttaatatgga cggcaagata atcaaagaac
     1981 atctccggcg tcatcgcacg cacggtgtct gggctggcag tatttggtgt tggtaatttt
     2041 tgtacgccat tacgcaattc ctgtgcacca gtgagataga aattacgcca cggcccagat
     2101 tcagcctgat agcctaactg ctcaagcgca tcagcttcca gatctctcgc tgcctggttg
     2161 tttggatcgg caaacaccac cttactaaca acttgggcaa cccagcggaa attaccctgg
     2221 tcataatcct gtgtggcttt ctgtaaaatg gcatcagcac cgcccatata ctcgacgaat
     2281 ttttttgcgc cttcttctgg cggcagttca tcaagcgtcg cggggttacc atcaaaccag
     2341 ccgagataaa gcacgtaagt tgctttcacg tcatgactta ccgaaccgta atagccgcga
     2401 ttcgcccagg tgttagccag tgaagagggc agtttaaagt ttgcggcaat ttcatcacgg
     2461 gtcatccctt gattagccat gcgcagagtt tgatcattga tgtaacgata caaatcacgc
     2521 tggcttttaa gcagtttgac cacgttatcg ttgccccagg ttggccagtg atgttgagcg
     2581 agaataatct cggctttatc accccacaaa ttgagagctt cattaatata ttttgaccac
     2641 ggcagcggtt ggcgaatttt agccccacgc agcgaatagg tgttatgaag ggtgtgcgtg
     2701 acatcttcag cggtttcgat cagtttcttt tcttcaataa accacagcat ttcagaaggc
     2761 gcttcagaac ctggcgccat caggaaatca taagtcaacc cgtcaatagt ttctatttgt
     2821 ccggttttgg tgatgtagtt agtaggagca atcaacgtga ctgttcctgc tgaagtagtc
     2881 gttcccagtc cagcccccac ctggccttta acatcaggtt tcaataagtt gccatacata
     2941 taactggctc gtcggctcat aacattgcca gccatgatgt tttcggaaac ggcttcctcc
     3001 aaaaatccgg ctggcgcata gattttaact ttgcctgatt taacgtcagc ttcatcaatt
     3061 acgccacgaa caccaccgta gtgatcgaca tgactgtgtg tatagataac cgccactacc
     3121 ggttttttgc cgcgattttt gtaatagaga tccataccca ctttggctgt ttctgctgaa
     3181 acaaggggat caacgacggt aataccctct ttgccttcaa tgatggtcat atttgataga
     3241 tcgagattgc gaatctgata gacaccttca gttacttcga ataacccgct gatatttatc
     3301 agttgcgcct gacgccataa actcggattc accgtatcag gggctttatc gccttctttg
     3361 ataaaagcgt aatgttgtgg atcccagata acattccctt gttcgccctt aatcacgtct
     3421 tgagggatag gggcaataaa acctttatgg gcatcagtaa agtcagtgtt atcggcaaac
     3481 ggcaactggt tgtagagtaa attgtttgct tgttgcgtgg ctgcggtggc gtctttagga
     3541 gcttccgccg cgaggagagg taaagaactg gtggtgaata atcccgttat tagcagactt
     3601 ttaacgatat gattaagctg catcttaagc gtctccagaa tggattttta ttgggggtaa
     3661 ttacggatga aaatttttag tgagggcaag aataactata atgggcgtta acaaattggc
     3721 aagccgggaa attgatgttt tcttaatggc atgttggcga agaatatatt ctgcaagtta
     3781 acgttttcat tggcgcttta tttaaaagtg tgtagtacaa atatgtacaa tttcaagtag
     3841 gcattattgc cgtaaaaacg cattaccgga cacacgacat tcacaacgtg tgttaatttt
     3901 actgacatct ttcaaaagga gcacaatcat gccgcacata gacattaaat gttttccgcg
     3961 tgaactggac gaacaacaaa aagcagcact tgctgcagat attaccgacg ttattattcg
     4021 ccatctgaac agtaaagata gctcgataag catttctcta cagcagattc aaccagaatc
     4081 ttggcaagct atctgggatg ccgaaatcgc gccccaaatg gaggctttga taaagaaacc
     4141 cggttacagc atgtaatgct taacctgccg taacacgctt gccgctggta acaaacgtgc
     4201 cagcacctaa atgatgcaac gtattgagtt tgtcatcatc aaactgccag cggccttcga
     4261 caaatactcg tgcgtctgcc gctgcagata ccacttcgcc aaatagcgta tcgtattttt
     4321 cttgcgcgga agttgcaggt agcaggcgac actccatcca cgccagacat ttttcctcga
     4381 ccagcggcaa accaagcacc ggacctttca caaccggaat accatagcaa ttaaacttat
     4441 cttcatcacg ccccgacaca cttcccaccg cccacgtcca gttagttgct gcaacacccg
     4501 ggataacgat gccaaactta ccattacgtt caatcaactc cctggtccat gtcgatttat
     4561 ctaccacaat cgccacgcgt ggcggttcaa actccaccgg cattgaccag gcggctgcca
     4621 taatgttacg ccgctgggat tgctcatcga aactggtgat caagacagtt gggccatgat
     4681 ttaacagacg gctggcatgg tgtaattcga cagggatgaa tcggctcacg gtatcgcctc
     4741 gctatgaagg gtgttttaaa agcgtagctc gtaacgcaat aagtaacgaa attaacggga
     4801 ttggcgattt gcgaacgtga tgcatgtccg cgatcgcaca aaatagccgg tgcagcgtct
     4861 attccaggtt ataagttgag aaaaccacta agggaaacgc ctgatgacgc ccattctgaa
     4921 tcactatttt gcccgtatta actggttggg agctgctgcg gtcaatatag atacgctgcg
     4981 tgcattgcac ctgaaacaca attgcaccat tccgtttgaa aacctcgacg ttttgctgcc
     5041 gagggaaata cagcttgatg atcaatcgct ggaagagaaa ctggtgacag cccgtcgtgg
     5101 tggttactgt tttgagcaga atggcgtgtt tgagcgggtg ttacgcgagc tggggtttaa
     5161 cgttcgcagc ttgttagggc gcgtagtgtt atcaaatccg ccagcattac cgccgcgcac
     5221 ccatcgtttg ttgttggtgg aagtggaagg ggaaaaatgg attgctgatg tcggtttcgg
     5281 tggacagacg ctaaccgcgc cgattcgatt agttcccgag ctcgtgcaga ccacgccaca
     5341 cggagagtat cggttgttgc aggagggtga tgattgggtg ttgcaattta atcatcatca
     5401 gcactggcag tcgatgtacc gttttgatct ctgcgagcag caacaaagcg attatgtaat
     5461 gggcaatttc tggtctgcgc actggccgca gtcgcatttt cgccatcatt tgctgatgtg
     5521 ccgccatttg ccggacggcg gcaagctgac actgaccaat tttcatttta cccattatga
     5581 aaatgggcac acggtggagc agcgaaatct accagatgtg gcgtcgttat atgccgtgat
     5641 gcaggaacag tttggtctgg gcgtggatga tgtgaaacat ggctttactg tggatgagtt
     5701 agcgctggtg atggctgcgt ttgatacgca cccggaggcg ggaaaataat ttatgtcagg
     5761 ttgccggatg cggcgtaaac gccttatccg gcatacatag acctgataag cgaagcgcat
     5821 caggcaacgg tttcagaatt caattgccca ttcggcatga aacaaaatca ccgctgtgcc
     5881 agaaatggtg actttctccg gttggttatc gcgaattgtc accgtcactt caatcacgcc
     5941 gtcacgccct aatgcgcgcc cctgatggcc tttaacatgc aacacgttgc catcgtgggg
     6001 caatacgttg tgatgtacca accatgcacc catcgggcca ttggcattgc cggtcaccgg
     6061 atcttccaca ataccaatgg caggcgagaa catacgaccg tcggtttcgt ttttgcctgg
     6121 gcggatctgg aacgggaaga aaccattgca gccaatctgt ttgctgatag cggtcagcgc
     6181 attaaggtca ggcgaaaggg cgtcgatatc cacttccggt ttcagtggga tcatcacttt
     6241 tgagtgtcct gtggttgcca cctgaattgg caagcctggc agaatatcgt cttcagtgag
     6301 atgaagcgcg ttgataatcg ccgcacgtgt ttcaccttcc agcggcggtt caaagcccgg
     6361 cggaccttgt tccagcgaaa tacgataatc atcgttgtgc ttttcgatag tcacgcgatg
     6421 ttttcctgcc agtgacgttt gccagatcgt gcaatttcct aaacctaaca ccttcgcacg
     6481 tacatagtgc gcagctactg tcgcgtgacc gcaaatcggc acttcaaccg ttggcgtaaa
     6541 gtagcggatg cgtacatcgc tgtcgtcgct gtgcagcaga aaagcagttt ccgaatgacc
     6601 taattcgcgg gcgataagct gcatttgcgc ttcactgaga ttatcggcgg ggaaaacaac
     6661 accggcagaa ttgccgcgaa acggttgtga ggtaaaagca tcgacgtggt acacctgcgg
     6721 tttcattaac gttctcctgt ggctggagaa ctatcatagc ctgcaagtgg ccggagagcg
     6781 aagggctatc cggccagggt gaaattatcg ccgcgagcgc acaatctgat accgacgagt
     6841 aaagtactca aacggcgcgc tccacacatg caccagtcgg gtgaacggga agagcaggaa
     6901 gatggtcatt cccaacacca gatgcaggcg gaaaacgaac gctacgccgt taagcatttc
     6961 tgacgatcca ccacggaaag tgacaatgct ttgcgcccaa cccaccagct tcatcatttc
     7021 gctaccatca ggatactgtg ctgaaaacgg tatggtactt agccccagca gacactgaat
     7081 cagcaaaatg atcatgatga taatatccgg cgtagtggaa gtggcacgca cgcgctgatt
     7141 tgtcagcctg cgccacagca gtcctgcgcc gccaatcagc gtcaatacac cgcaaatacc
     7201 accgagcacc atcgccatct gctgttttac cgccaccggc agaaaccacg cgtacatcca
     7261 gtgcggcgtt aacatgccga acaggtgccc gaagaaaatc cccaaaatgc cgatatggaa
     7321 cagattcgac catatcacca tcccgcgttt atcgagcatc tgactcgagg aggcgcgcca
     7381 ggtatactgc ccgtagtcgt agcgcagcca gctgccgagg aaaaacaccg tcgcacaaat
     7441 gtacgggtag atatcgtaaa aaaagacgtt cagatactga atcatttccc acctcccgca
     7501 ctgatgtcga catactgcgg cgcgacatcc tggctaaagc gtcgctgata ttgattaagc
     7561 ggcgagctgt cacacgccgt ggcgttatct tcaataaact tcacctgttc ctcttcccac
     7621 accgcatcca gtgcctggcg ggtgtcatcg cgctcctcac tgttcacctg ttttgtgaca
     7681 ctgtcacttg aaagtgtact tcctgccagt tgcaacaggg tgtcaaataa cgcgtaccac
     7741 gatgcctcgc gttgttttaa gcgtccgcca agcagggcga ggatcggcgc aacgttgagc
     7801 aagccttctt tcgcctgatc gtccggcagt acgcttaagt actccagata cagcggtaga
     7861 taatcgggca gttctcgaca atccaactgc aggccgacct tttcatactc cgccagcagg
     7921 tccaccattg cctggccgcg atcgcgggac tcggcatgaa catgttcgaa cagcagcagc
     7981 gatgtggtac gtccacggtc aaacacttcg caccattcgg cctgtttatc cagcagcggc
     8041 gcgttaagca ggttgcgggt aaaatccgta agcatcggtg cgtcgcggcg gatcaacgcc
     8101 agcgcgtcct ccttacattc ccacaacagc tcgtctggat actccatcag caggccgatc
     8161 actttgagga tctgcattat tcgccctccg ctttgtcgcg cacttcggtg atgttgatgg
     8221 catcgatacg gctactgttg aacaggttga atttactgtc cgaaccgtgg caaccgtcgc
     8281 caaaggtaaa accgcagcca ttgcgttctg cgaaggcatc gcccgccatt tcccgatggc
     8341 tggtcgggat aacaaaacga tcttcatagt tggcaatggc gagatagcga tacatttctt
     8401 cgacctgggc gacgctcagg cctacttcgt cgatggcacg agtatcagta acgccttcca
     8461 cggtttgtga acgcatatag tggcgcatcg ccatcatccg tttcagcgcc cgcagtaccg
     8521 gaccggtatc gccggcactc aacatattgg cgagatattg caccggaata cgcaggcttt
     8581 cgatggcggg cagcacgcct tcgcttttcg gcaaaccgcc cgcatctgcg taggactgaa
     8641 tcggtgacag cggaggaacg taccagacca tcggcagggt gcggtattca gggtgcagcg
     8701 gtaacgccag tttccagtcc atcgccattt tgtagactgg cgaacgctgg gcagcgtcaa
     8761 tcacattttg tggaatacct tgtttcaggg cttcttcgat cactgaggga tcgtgcggat
     8821 cgaggaacac ttcgcactgg cgttcataga ggtcaacttc gcgctcggtg ctcgccgctt
     8881 cctcgatgcg gtcggcgtcg taaagcagca cgcccagata ccggatgcga cccacgcagg
     8941 tttctgagca cacggtgggt tggcctgact caattcgtgg gtaacagaag atacattttt
     9001 ctgacttgcc gcttttccag ttgaagtaga tttttttgta cggacaaccg ctaatgcaca
     9061 aacgccagcc acggcattta tcctgatcga tcagcacaat gccgtcttct tcgcgtttgt
     9121 agatagcgcc gcttgggcag gtcgccacgc aactgggatt gaggcagtgt tcgcacaagc
     9181 gcggcaggta catcatgaag gtgttttcaa actgcccgta catctccttt tgcatggcct
     9241 cgaagttgcg gtcatgggcg cgtttttcga attcgccgcc caacaattct tcccagtttg
     9301 gcccccagat cactttgtcc atccgtttgc cgtcaatcag tgaacgcggg cgagcagtag
     9361 ggatatgttt gccttccggt gcgctatgca aatgttcgta gtcgaaggtg aaaggttcgt
     9421 agtaatcatc aatctgcggc accaccgggt tggcgaagat tttggttatt acgcccatct
     9481 tgctgcccag acgcgggcgg atcttgccat tcacatcacg cacccagccg ccttgccact
     9541 cttcctgatc ttcccagttt ttcggataac caatgcccgg tttggtttcg acgttgttaa
     9601 accatgcgta ctccatgcct tcgcgaccgg tccagacgtt tttacaggtc accgaacagg
     9661 tatggcagcc gatacattta tcgagattaa gcaccatgcc gacttgcgaa cgtattttca
     9721 ttttttcgcc tcctgtacct gatcgcgacc ttcatcatcc agccagttaa cgttcttcat
     9781 cttgcggatc atgatgaact cgtcacggtt cgagccgacg gtaccgtagt agttaaagcc
     9841 ccaggccagc tgcgcgtaac cgccaatcat atgcgttggt ttcgggcaaa cgcgggtgac
     9901 cgagttatga atgccgccgc gcatgccagt tacttccgaa ccaggaatat tcataatgcg
     9961 ttcctgggcg tgatacatca tggtcatgcc cggcggtaca cgttggctga ccaccgcgcg
    10021 ggccgtcagc gcgccgttgg cgttaaatac ttccacccag tcgttatcga caatggtcag
    10081 ttctcgggca tcggtttcgc tgatccagac aatcggtcca ccgcgagaga gcgtcagcat
    10141 tagcaggttt tcactgtagg ttgagtgaat gccccatttc tggtgcggcg tcaggaagtt
    10201 aagtgctttt tccgggaagc cgtttggcgg gatctggcgc atctcactga cgctacgggt
    10261 gtcgataggc gggcgataag ccaccaggct ttcaccaaaa gcacgcatcc acggatgatc
    10321 ctgatagagt tgctggcgtc ccgacagcgt gcgccacgga attaactcat gaacgttggt
    10381 gtatcccgcg ttataggaga cgtgatcgct ttccagacca gaccatgtgg ggctggagat
    10441 aattttacgt ggctgcgcct gaatatcgcg aaagcgaatc ttctcgtcct ctttgtgcag
    10501 cgccagatgg gtatgttcgc gtccggtgat ctcgcccagc gcctgccacg ctttgactgc
    10561 aacatgaccg ttggtttccg gtgccagagc cagaatcact tctgatgcgt caatggcggt
    10621 gtcaatcagc ggacgcccct gtgccgggcc ttcacgcttg gtgtagttga gtttgccgag
    10681 gaaatcgatt tcatcctggg tattccacga aatcccttta ccgccgttgc caagtttgtc
    10741 catcaatggc ccgagcgagg taaagcgttc ataggtagca gggtagtcgc gttccaccgc
    10801 cacaatattg ggcgcagttt tacctggaat cagatcacat tcgcctttgc gccagtcgag
    10861 cacttcacac ggctgtgaga gttctgccgg agagtcatgc agcagtggtt gtaataccac
    10921 gtcggtttct ttgccaagat ggcccacgca cacttgcgaa aaggctttgg caataccttt
    10981 gtagatttcc cagtcgctgc gtgattccca cgccggatcg accgccgcag aaagcggatg
    11041 aataaacgga tgcatatccg aggtgttcat atcgtctttt tcgtaccagg tggcggtggg
    11101 cagaacgata tcggagaaca ggcaggtact ggacatacgg aagtcgagcg tcaccagcag
    11161 gtcgagcttg ccttcaatcg ccgcagtttg ccactcgact tcttctggtt tgatcccgtc
    11221 gctggcaccg agttcctgac cctgaatccc actttcggtt cccagcagat acttctgcat
    11281 atactcgtgg cctttgccgg aggagccaag caggttagaa cgccagacaa acaaattacg
    11341 cggatgattg ctgccgctat ctggttgttc gcaggccatg cgtaaatcgc ccgatttcag
    11401 cgcctgggcg gtaaattctg tgggggataa tccggccttg tcggcttcag ctttaatccc
    11461 gagcgggtta cgccccagct gcggcgcaga aggtagccag cccatacgtt cggcacgaac
    11521 gttgaaatca atcagatgac cgctgtactt actggcatcg gcgagcggtg aaagtaactc
    11581 ctgagcagag attttttcat agcgccattg gctggaatga ttgtagaaaa acgaggtgct
    11641 gttcatctgg cgcggtgggc ggttccagtc gagcgcaaag gccagcggca accagccggt
    11701 ttgtgggcgc agtttttcct gaccgacata gtgtgcccat ccgccaccgc tttgcccgac
    11761 acaaccgcag aagatcagca tgttgatcat cccacggtag tgcatgtcca tgtggtacca
    11821 gtggttaaca cctgcgccga ggataatcat cgagcgccca tgcgttttat gggcagtatc
    11881 ggcaaattca cgagcgatgg tttcaatata ctggcgcggc acaccggtaa tttgctcacc
    11941 ccaggctggg gtgtacggtt tgatttcagc gtaatctttc gcgctgtttt catcttccag
    12001 cccgcgatcg aggccgtaat tcgccagtac caaatcataa acgctgacca ccggacaggt
    12061 gctgccatcg gctaatgtca ggtttttaac gggtaattga cgcaccagga caggattgtt
    12121 ttttacgctg cgaaaatgcg gattttcaat gccgccaaag taggggaagg ccacgcctgc
    12181 aacggcatca tgttgaccga gcagagttaa cgacaattcg gtttccgtac cggcggcaat
    12241 ggattccaga ttccatttgc ctttttctcc ccagcgaaaa ccaatcgaac cgttcggcac
    12301 caccagttca cctgcggtat taaccgccac ggttttccac tgcggattgt tgctttcgcc
    12361 cagtccatcc accaggtcag atgcgcggac catgcgcccg ggaacgtagc tgccgtcgtc
    12421 gcgaggttcc agcattacca gcatcggcat gtcgctgtag cggcggcagt agttgataaa
    12481 ataatcgctg ggattatcga gatgaaactc ttttaaaatg acatggccca ttgccattgc
    12541 cagggcacta tcagtgcctt gtttcggtgc cagccactgg tcgcacaatt tggccacttc
    12601 agagtagtca ggggtaatgg cgatggtttt agtgcctttg tagcgtactt cggtaaagaa
    12661 atgggcgtcc ggcgtacgtg tctgcggtac gttagacccc caggcgatga tgtaactgga
    12721 gttataccag tcggcagatt ccggcacgtc ggtttgttcg ccccaggtca tcggcgaggc
    12781 gggcggcagg tcgcaatacc agtcgtagaa gcttaaacag gtgccaccta atagtgaaag
    12841 ataacgtgtt ccggcggcgt aagaaaccat cgacatcgcc gggatcggcg agaaaccggc
    12901 aacgcgatcc gggccgtaag ttttgatggt ccagacgtta gcagcggcaa tcagctggtt
    12961 tagctcctgc cagttggagc ggataaagcc gccgcgtcca cgtacttgtt tgtagcttag
    13021 gcacttttgc ggatcgttca taatcgatgc ccacgccagt accggatcgc tgtgttgctt
    13081 gagggcttcg cgccacagtt caatcagtcg tttacgaatg agagggtatt tcaggcggtt
    13141 agcgctgtag agataccagg agtaacttgc gccgcgcggg cagccgcgag gttcatgatt
    13201 gggcaggtca gggcgagtgc gcgggtagtc ggtctgttgg atttcccagg tcaccagacc
    13261 atttttaacg tagattttcc agctacagga gcctgtacag ttaacaccgt gggtggaacg
    13321 cacgattttg tcgaactgcc aacgctggcg atagctgtcc tcccagtcgc ggttgctatg
    13381 catcacctgt ccgtgaccat cggcaaaggt ttcgcccttt tgtttgaagt agcgaaagcg
    13441 atccaaaagt ttactcatga cataactcct gttccaggaa tgaattggga tctgatgcct
    13501 tgtcggatgc gacacggttg tagcctgata agacgtgtca ggcatcgatc tcttattttt
    13561 ggctgaactt ccgccgacca taaaccagcc aggtcagcag cacacaaacg atgtagaaga
    13621 ttaaaaacac tttcatcgcg cctaccggag agccggtcat attgagcgac atgccaaacg
    13681 cctgcggaat gaaaaagccg cccactgcgc caatggctga gataaagccc agagccgccg
    13741 ccgtttcggt gacggcttct ttctgagctt gctcatcact accgcctttc atcttcaccc
    13801 gataaatggt tatctggcga aagatgacgg cgatcatctg gaaagtagaa ccacttccca
    13861 gacctgcggt cagaaacaac cccataaata cggcgtaaaa tgcgatgaaa ttaccggagc
    13921 ctgtgcccgg taaggtaagg aacagcaggg cgctgaaaat cgccataaaa atgaagttga
    13981 tcaacgtcac ccgcacgccg ccgaatttat cggaaatagc accaccaacc gaccgcgcga
    14041 tggcaccaat aaatgggcca aagaacgcca ggcgcagaat attcacatca gggaactgag
    14101 ttttcgccag catggcaaaa cccgcagaaa aaccgataaa cgaaccgaag gtggcaaggt
    14161 aaagcaggct cagcagccag agatgcaggc gttgtaacac tggcagctgg tcggcaattg
    14221 acgcgcgtga actggcgata tcattcatcc ctgaccaggc ggcgatcgtg gcaatcgcca
    14281 gtaatggcac ccaaatccat gcggcattcg ccagcgacat caccgaaccg tcggcctgcg
    14341 gtacgccatt gacgccgaga aaggcaaata cgggtacaaa aatgaccagc ggtgcaacca
    14401 gctgcatcac acttacacct aagtttccta acccgccatt aatcccaagt gcgctccctt
    14461 gtttggcttt tgggaagaag aaactgatat tgcccatgct cgaagcaaag ttcgcacctg
    14521 caaaaccgca tagcaaagcg ataacgataa atatcccaaa aggagtattc ggattctgca
    14581 cggcaattcc gagccagacg caaggaataa tcaggattgc agtactaaaa accgtccatc
    14641 ggcgtccacc aaatataggc accataaagg agtaggggac acgcaataat gcgccagaaa
    14701 cggagggtaa tgcggttaat aaaaagagtt gatcggtagt gaaattaaaa ccgattttat
    14761 tgagattaac agtaactgcg ctaaatagca tccagacaca gaaggcaaga agtagacaac
    14821 tgactgatat ccagaggttt cttcgagcaa tatgttttcc tttattttcc cagaaggccg
    14881 gattttctgg tttccagtcg cgcaaaagat aacgactagt tttctcattt tgcagtgcca
    14941 tattgttcct cacatgtaca cattggtaat gaaaaaaaga caaaacagga ggtaaggcgc
    15001 aatagccagt tattagaatt aaggatgaat taggtgaagt gctgattgaa agaatagata
    15061 agaaagcgta accgcggggg cagaatgggg attaaacaga cagatatgtg ttactaaatg
    15121 taactaacaa gctaaccatt aaacggatgt taagctatgt atgcatgtca tgatccaacc
    15181 cttcagatga tgaatccaaa gggtttatat ttttaacaag accagacact tatagaatta
    15241 gtgataattt attcactaaa aaggccatga gctggaggaa aacgttctgt ccagaaagtc
    15301 tatcggttcc tctcttatcg cctcattgta ataatcgtta ttacagtaag tattgtttat
    15361 ttcatctaag gtcatatttt taatttttat attcct
//
LOCUS       DACBVI010000107        15108 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.107, whole genome shotgun sequence.
ACCESSION   DACBVI010000107 DACBVI010000000
VERSION     DACBVI010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.107"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..360)
                     /locus_tag="IU325_16145"
     CDS             complement(<1..360)
                     /locus_tag="IU325_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810531.1"
                     /translation="MMKTITKQPILFTDVPVADLRNSMKHDLNQNLIERLWNKIRDFF
                     LGSDKQKAFKSIHKYINTLSVLNYNSASSPDPNFNIDASSDLDSYLNLKFDNLSPKQK
                     QTTLCCFWNKIASSLPEP"
     gene            complement(591..1565)
                     /gene="yajO"
                     /locus_tag="IU325_16150"
     CDS             complement(591..1565)
                     /gene="yajO"
                     /locus_tag="IU325_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HAP5810532.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEGIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(1620..3482)
                     /locus_tag="IU325_16155"
     CDS             complement(1620..3482)
                     /locus_tag="IU325_16155"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP5810533.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(3507..4406)
                     /gene="ispA"
                     /locus_tag="IU325_16160"
     CDS             complement(3507..4406)
                     /gene="ispA"
                     /locus_tag="IU325_16160"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HAP5810534.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            complement(4406..4648)
                     /gene="xseB"
                     /locus_tag="IU325_16165"
     CDS             complement(4406..4648)
                     /gene="xseB"
                     /locus_tag="IU325_16165"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015571292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP5810535.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            4854..6302
                     /gene="thiI"
                     /locus_tag="IU325_16170"
     CDS             4854..6302
                     /gene="thiI"
                     /locus_tag="IU325_16170"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP5810536.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(6356..6946)
                     /gene="yajL"
                     /locus_tag="IU325_16175"
     CDS             complement(6356..6946)
                     /gene="yajL"
                     /locus_tag="IU325_16175"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HAP5810537.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETIKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6909..7820)
                     /gene="panE"
                     /locus_tag="IU325_16180"
     CDS             complement(6909..7820)
                     /gene="panE"
                     /locus_tag="IU325_16180"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP5810538.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            7988..8479
                     /locus_tag="IU325_16185"
     CDS             7988..8479
                     /locus_tag="IU325_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP5810539.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(8607..9971)
                     /locus_tag="IU325_16190"
     CDS             complement(8607..9971)
                     /locus_tag="IU325_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5810540.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETAGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(10120..11010)
                     /gene="cyoE"
                     /locus_tag="IU325_16195"
     CDS             complement(10120..11010)
                     /gene="cyoE"
                     /locus_tag="IU325_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HAP5810541.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGRIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(11022..11351)
                     /locus_tag="IU325_16200"
     CDS             complement(11022..11351)
                     /locus_tag="IU325_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HAP5810542.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(11351..11965)
                     /locus_tag="IU325_16205"
     CDS             complement(11351..11965)
                     /locus_tag="IU325_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HAP5810543.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(11955..13946)
                     /locus_tag="IU325_16210"
     CDS             complement(11955..13946)
                     /locus_tag="IU325_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HAP5810544.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(13968..14915)
                     /locus_tag="IU325_16215"
     CDS             complement(13968..14915)
                     /locus_tag="IU325_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HAP5810545.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMNMTQPEGEHSAHEGMEGMDMSHAESAH"
BASE COUNT         3767 a         3961 c         3861 g         3519 t
ORIGIN      
        1 tggctctggc aatgaggatg caattttatt ccagaaacaa caaagcgtgg tctgcttttg
       61 tttgggcgag aggttatcaa atttcaggtt taaatatgag tctaaatctg acgatgcatc
      121 tatattaaaa ttgggatctg gagatgacgc tgaattataa tttagaactg acaatgtatt
      181 gatgtattta tgaatcgatt taaatgcttt ttgtttatca ctgcccagaa aaaaatcacg
      241 aattttgttc catagccttt cgataagatt ttgatttaaa tcgtgcttca ttgagtttct
      301 taaatctgca accggtacat cagtaaacag gatcggttgt ttggtgatag ttttcatcat
      361 gattgtaatc ctcttacgtt gactaaataa gacagtttta gggagtatta cctggagaaa
      421 ctatcaaaaa tcgctcaaag ggatgaagat gatgaatagc tgcaaactca ttcacgaaga
      481 atttctcttg cagaggtgga ttatggttac agaagatgat gggaggtacg ttgtccccat
      541 catcgcagac gatggggaat tcagcagata aaatggctga aaagcgaaat ttacttaaat
      601 cctactaccg gatgcggttt atacggcgtt tccagttcag caatctgttc cggcttcaag
      661 gtgatatcca ccgcgttcag cagctcatca agctgttctt cccgcgaagt tccgataatc
      721 ggtgcggcaa tgcccggttt actcaacaac caggccagcg caacttgtgc tcgtgttgca
      781 cccagttctt cactgacgcc cgttaaccgc tctgcgatct gcgcgtcatt ttcatcgctt
      841 tctttataga gatttttccc cacctcatca gacaccagtc gtgcggtagt ttctccccac
      901 ggacgtgtca gacgaccccg cgccagcggg ctccatggaa tcaccgctac gccctcctgg
      961 taacacagtg gcagcatctc gcgctcttct tctcgataaa tcagattgta gtgatcctgc
     1021 atactgacaa actgcgccca gccgtgctgt ttttgtagtt ccagtgcctg agcaaactgc
     1081 gaagcgtgca tggatgacgc gccgatataa cgcgctttcc cggcttttac cacgtcgttg
     1141 agggcttcca gcgtctcttc gatcggcgtg ttgtaatccc agcgatgaat ttgcaggata
     1201 tcgacataat ccatgccgag acggcgcagg ctgtcgtcga tagagcgcaa tatttgcgca
     1261 cgggataatc cttccggcaa atcaccaacg cgatggaaca ctttggtggc aacgaccacg
     1321 tcttcacgac gggcgaaatc ccgcagtgcg cgaccaacga tcccttcgct gctgccatcg
     1381 gaatagctat tggcggtatc aaagaaattt atgccgcctt ccagcgcgcg tttaatgatg
     1441 ggacggctgc tttcttccgg cagtgtccat gcgtgattac cacgatctgg ctcgccaaag
     1501 gtcatacagc cgaggcaaag tcgggaaacg cgaaggtcgg tttttcctaa ggggttgtat
     1561 tgcatgctgc cactcctgcc atgaataatt tcttaagcat agcaggagta gagtagggat
     1621 tatgccagcc aggccttgat tttggcttcc ataccggcgg catcgaggcc gagttcggcg
     1681 cgcatttctt cctgtgttcc ttgtggaata aagaagtcag gcaggccaat gttcagcacg
     1741 ggtactggtt tacgatgggc catcagcact tcgttcactc cgctgcctgc gccgcccata
     1801 atggcgtttt cttctacggt gaccagcgct tcatggctgg cggccatttc cagaattaac
     1861 gcttcatcaa gcggtttcac aaaacgcata tcgaccagcg tagcgttcag cgattcagcg
     1921 actttcgccg cttctggcat cagcgtacca aagttaagga tcgccagttt ctcgccacga
     1981 cgcttcacaa tgcctttgcc aattggcagt ttttccagcg gcgtcagttc cacgccaacc
     2041 gcgttgccac gcgggtagcg taccgctgac gggccatcgt tatagtgata gccggtatag
     2101 agcatctggc gacattcgtt ttcatcgctc ggggtcataa tgaccatttc cggaatacag
     2161 cgcaggtaag agagatcaaa agcgccctgg tgggtttgac catcagcacc aacaatgccc
     2221 gcgcggtcga tggcgaacag gaccgggagt ttttgaatcg ccacgtcatg cagcacctga
     2281 tcataggcgc gttgcaggaa ggtggagtag atcgcgacaa tgggtttgta cccaccaatc
     2341 gccagacccg cagcaaaggt cactgcgtgt tgctcggcaa ttgccacatc gaagtagcga
     2401 tccgggaatt tacgtgaaaa ctcgaccatg ccggaacctt cacgcatcgc cggagtaatc
     2461 gccatcagct tgttatcttt cgctgccgtt tcgcacaacc agtcgccaaa gatttttgaa
     2521 tagcttggta aaccgccgct acttttcggc aaacaaccgc tggagggatc aaatttaggc
     2581 acggcgtgga aggtgattgg gtctttttct gccggttcat aaccacgacc ttttttggtc
     2641 atgatatgca ggaactgcgg gcctttcagg tcgcgcatgt tcttaagcgt ggtgataagc
     2701 cccagcacat cgtgaccgtc aaccgggccg atgtagttaa agcccagctc ttcaaacaac
     2761 gtgccaggca ccaccatgcc tttaatatgt tcttcggtac gtttgagcag ctctttaatg
     2821 gggggcacgc cagagaaaac ttttttcccg ccttcgcgca gtgaagagta aagcttaccg
     2881 gaaagcagct gcgccagatg gttgttgagc gcgccaacat tttcggaaat cgacatttca
     2941 ttgtcgttga gaaccaccag catatcagga cggatatcgc ccgcgtgatt catcgcttca
     3001 aacgccatgc cagcggtaat cgcaccgtcg ccaatcacac aaacggtgcg gcgatttttg
     3061 ccttcttttt cggcagcgac cgcaatacca attccggcac tgatggaggt tgatgaatgc
     3121 ccgacgctta atacgtcata ttcgctttcg ccgcgccacg ggaacgggtg cagaccgcct
     3181 ttctgacgga tggtgccgat cttgtcgcgg cgtccggtca aaattttatg cggataagcc
     3241 tgatgcccca catcccaaat caattggtca aacggggtgt tgtagacata gtgcagcgcc
     3301 acggtcagtt cgaccgtgcc cagcccggag gcgaagtgcc cgctggaacg gctcacgctg
     3361 tcgagtaaat agcggcgcag ttcgtcgcag agtttcggta aactctcttt cggcaacagt
     3421 cgtaactcct gggtggagtc gaccagtgcc agggtcgggt atttggcaat atcaaaactc
     3481 atcaggggcc tattaatact tattgtttat ttattacgct ggatgatgta gtccgctagc
     3541 gcttccagtg ccgaggtatc gagtgactgc tcagccagtt gtttcagcga ctgacgggca
     3601 tcgtcgatca gatcccgggc tttcttccgg gcttgctcaa gacccagaag tgcagggtag
     3661 gtacttttac caagttgctg gtcggcaccc tggcgttttc ccaacgttgc agtatctcct
     3721 accacatcca ggatgtcatc ctgaacctgg aaggcaaggc cgatgctctc tgcgtacttg
     3781 tcgagtactg gcagagcacg acgtccttta tctccggcgc ttaatgcacc aaggcgaacg
     3841 gcggcgcgaa tcaatgcgcc ggttttatga cgatgaatac gctcaagcgc gtccagaggt
     3901 acgtgtttgc cttccgcgtc taaatctaat gcctgaccac cgcacattcc ggcaataccg
     3961 ctggcgctcg ccagttctga aatcatcgaa attctgtcgc gatccgacac ttccggcata
     4021 tcggcatcgc ttaaaatcga gaacgccagc gtttgtaaag cgtcaccagc gagaatcgcg
     4081 tttgcttcgc caaacttcac atggcaggtt ggcaaaccgc gacgcagatc gtcatcatcc
     4141 attgccggta aatcatcatg aattaatgag taagcgtgga tacactctac ggcggcagcg
     4201 ggcgcgtcca gcgtgtttgt gctaacgccg aacatatgac cggtggcata aaccaggaaa
     4261 ggtcgcaggc gcttaccacc taataatgcg ccatactgca tggtttcgac cacgggagtg
     4321 ttctgaaagg gcagtggggc gataaaacgg ctcagcgcct ggttggcctg cttaacgcag
     4381 gcttcgagtt gctgcggaaa gtccattact cattgtccgg tgtaaaaggg gttagagagg
     4441 cgtcttcatt gtcagacagc agaatttgta cgcgctgttc ggcttgttgt aatttggcct
     4501 gcccctgacg tgccagctgc acgccgcgtt cgaactcgtt cagcgcctct tccagcggca
     4561 ggtcaccact ttccagacgg gttacaatct gttccagctc gctcagcgcc ttttcaaagc
     4621 tggcgggcgc ctcatttttc ttcggcataa tgaatgtctg actctcaata tttttcgccc
     4681 cgtcatggta acggactcag ggcaaatagc aaataacgcg caatggtaag gtgatgtgcg
     4741 cagcaaagcg atgttagtgg tatacttccg cgcctggatg cagccgcagg tgtgggctgc
     4801 tgtatttttc cctatacaag tcgcttaagg cttgccaacg aaccattgcc gccatgaagt
     4861 ttatcattaa attgttcccg gaaatcacca tcaaaagcca atctgtgcgc ttgcgcttta
     4921 taaaaatcct taccgggaac attcgtaacg ttttaaagca ctatgatgag acgctcgctg
     4981 tcgtccgcca ctgggataac atcgaggttc gcgcaaaaga tgaaaaccag cgtctggcta
     5041 ttcgcgacgc cctgacccgt attccgggta tccaccatat tctcgaagtc gaagacgtgc
     5101 cgtttaccga catgcacgat attttcgaga aagcgttggt tcagtatcgc gatcagctgg
     5161 aagggaaaac cttctgcgta cgcgtgaagc gccgtggcaa acatgatttt agctcgattg
     5221 atgtggaacg ttacgtcggc ggcggtttaa atcagcatat tgaatccgcg cgcgtgaagc
     5281 tgaccaatcc ggatgtgact gtccatctgg aagtggaaga cgatcgtctc ctgctgatta
     5341 aaggtcgcta cgaaggtatt ggcggtttcc cgatcggcac ccaggaagat gtgctgtcgc
     5401 tcatttccgg tggtttcgac tccggtgttt ccagttatat gttgatgcgt cgcggctgcc
     5461 gcgtgcatta ctgcttcttt aacctcggcg gcgcggcgca tgaaattggc gtgcgtcagg
     5521 tggcacatta tctgtggaac cgctttggca gctcccaccg cgtgcgtttt gtcgctatta
     5581 atttcgagcc ggtcgtcggg gaaattctcg agaaaatcga cgacggtcag atgggcgtta
     5641 tcctcaaacg tatgatggtg cgtgccgcgt ctaaagtggc tgaacgttac ggcgtacagg
     5701 cgctggtcac cggcgaagca ctgggccagg tgtccagcca gacgctgact aacctgcgcc
     5761 tgattgataa cgtctccgac acgctgatcc tgcgtccgct gatttcttac gacaaagagc
     5821 acatcatcaa cctggctcgc cagattggca ctgaagactt tgcccgcacg atgcccgaat
     5881 attgcggtgt gatctccaaa agcccgacgg tgaaagcggt taaatcgaag attgaagcgg
     5941 aagaagagaa gttcgacttc agcattctcg ataaagtggt tgaggaagcg aataacgttg
     6001 atatccgcga aatcgcccag cagaccgagc aggaagtggt ggaagtggaa accgtcaatg
     6061 gcttcggccc gaacgacgtg atcctcgata tccgttctat cgatgaacag gaagataagc
     6121 cgctgaaagt cgaagggatt gatgtggttt ctctgccgtt ctataaactg agcaccaaat
     6181 ttggcgatct cgaccagaac aaaacctggc tgctgtggtg tgagcgcggg gtgatgagcc
     6241 gcctgcaggc gctctatctg cgcgagcagg gctttaacaa tgtgaaggta tatcgcccgt
     6301 aatttgtgtt ttttacgtcg catctggtca gatgcgacgt ttgccgcatc cgacactact
     6361 cgtaataatt ataaatccct gccgccatca ccagttgtga tgccacttca tgggcttttt
     6421 cacgcccaac cagcaggtcg ataattttca gaccaaagtc gatagctgta cccggcccct
     6481 gactggtcag caattttacc cgtgcatccc agacgacgcg cttgtcctgc cattgttcgg
     6541 cgggtatttt gtctttcagc gtcgggaagc cggtcatatt gccaatcggg aagatatcgt
     6601 gcgggaccag cacggtggct ggtgcggcgc aaatagccgc gacgatacgc ccggaacggt
     6661 ggaactgttt aatggtttca accagcaggg tgctatcgcg aaaacactct gcgcctttaa
     6721 tgcctccagg cagcacgatc acgtcatatt cgccatcagc tacttcgacc agcggcgcat
     6781 ccgccagcag cttcacgccg cgcgagcagg taatcgccag gttaccatcg ctggcgacgc
     6841 tggcagtggt gactttgata ccgccgcgaa ccagcagatc gatagtggtg acggcttcag
     6901 tctcttcact accaggggcg aggcaaacca gtgccgatgc gctcatattc actctccttt
     6961 ctttttacca tttcaaacag gcgggtgttt tccggtacgg caatcccatg cgcgcgggcg
     7021 cggcgtaaga gaaaaccatt gatatagtcg atttcggtgt ggcgcagcgc gcggatatcc
     7081 tgcaacatcg acgagatatt ttccgctgtg gcatcaatca cctgcatcac gtaatcacgc
     7141 aaatcttctg ctgaagtatg atgcccttcg cgttcgatta ctgccgcgac ttcttcgcat
     7201 atctgcataa tttcttgcgg atgatgacgt aattcaccat tcgggcaatt ccagatggcg
     7261 gtcagtggat taatcacaca gttgactgcc agcttgcgcc acagctcggc gcgaatattg
     7321 ttatgccacg cgacgtcagg taacacggtt tgcaaaatat ccgccagata actgtaatcg
     7381 ccgtcctgtt gccgtgccgg accaatatgc gtgattccgt ttgccacatg aataatgaca
     7441 ttgccgtcgc ggcgggcggc atgggtggtg gtgcccatca gtaatggctg ctgaatgttt
     7501 tgcaactctt cgatggtgcc catgccgttg tgaattaaca gtattggcgt agttacaggc
     7561 agtgtggacg cgaggctttt gacggcatcg gaaacctgcc atgctttcag cgtcaccagg
     7621 agcagatcgc tggtggcgag aaaatcggga tcgttggcgg tcagcgattc gttaaatatc
     7681 gaaccatctg tctcaaccag attcacacta caataaggtt gtggtacgcg cagccagccc
     7741 tgaacctcgt gaccctgttt acaaagtgct gtaagccata attgccctaa ggcaccgcat
     7801 cccaatacgg taattttcat tgttcctcct cacccgcaac cactccgggt gttcaataag
     7861 gctatccctt aattgtgcat gctgttgcga ctatgcacaa ttaagggata cgtcctggtg
     7921 caggactgtt ggttatttaa ctttgcaggt attatgcttc gcatcaaaaa tgaagggaga
     7981 ggaaaatatg ccatctttcg atattgtctc tgaagttgat cttcaggaag cacgtaacgc
     8041 ggtcgataac gcgagccgcg aagtggagtc ccgttttgac ttccgtaatg ttgaagcctc
     8101 atttgagctg aacgacgcca gcaaaaccat caaagtgttg agcgagtccg atttccaggt
     8161 caatcagttg ctggatattc tgcgtgccaa gctgctgaag cgcggcattg aaggcagttc
     8221 actggatgta ccggaaaata tcgttcatag cggtaaaacc tggtttgtgg aagcgaaact
     8281 gaaacagggc attgagagcg cgacccagaa gaaaatcgtc aagatgatca aagacagcaa
     8341 actgaaagtg caggcgcaaa ttcagggtga tgagatccgc gtaacgggta aatctcgtga
     8401 tgatttgcag gctgtcatgg cgatggtacg tggtggcgat ctcggtcagc cgttccagtt
     8461 caaaaacttc cgcgattaat cgcgacaatg tgcgttttgt tcatgccgaa tgcagcatga
     8521 acagctaatt cggcctacaa cgatttgtaa ctcaggaact ttgtaggcct ggtaagcgta
     8581 gcgcagcagg caattcgcct cgttttttat gcctgacgaa ttgcctgttc aacctcaaag
     8641 cgattcgtca ctttgctgtc gattttcaca taagctgaat gttcttcttc tgcaatcaac
     8701 acttctttga cgcccgcggt ttccagcaaa cgcactttta acgcctcgtt tgcggcaatg
     8761 tccgccggga tttcaatgcg caagctgctg acatacggcg gttctttcat ggtactggcg
     8821 actgccagcc acactgcggc cagcattgcg ccagcgagaa atactccctg accgtcaaac
     8881 atgccgtcaa tccagccgcc cagcgaaccg ccaatcgcca cgccaagaaa ctggctggtg
     8941 gagtaaacac ccatcgccgt acctttgtaa cctgctggcg actctttact gataagtgaa
     9001 ggcagaaggg cttccatcaa attaaacgct acaaagaaaa gctgcacgcc gaccaccagt
     9061 tgccagaact gcgtttgtgc gttccacaac acaatttccg caaccacgat caacccaacg
     9121 cagaagacaa agacttgctt cattttgcgc ttaacttcag cgtagataat gaaaggcacg
     9181 accgagccaa aggcgattag cattgtcgcc agatagacct tccagtgttc agccgccggg
     9241 aaccccgcat cagccagttg tccgggcagg gcaacaaacg tcgacatcag caaaatatgc
     9301 agacacataa tgccaaagtt gagtttcagc agccgcggtt ccgccagcac tttactgaaa
     9361 ctgcctttca ccattccgga ctcacgatta agtacgtgag tgctactgtt gggcacaacc
     9421 caaatggtca acgcaatgcc ggtcgttgcc agaatagcga tcatccagaa cagcgcgtgc
     9481 agcccaagtt tgtgagtgat gatcgggcca agcaccatcg caatggcaaa ggtaatgcca
     9541 aagctcacgc cgataaacgc cattgctttg gtgcggtttt gttcgcgcgt gagatcggaa
     9601 agcagcgcca taacggcagc ggcaatcgca ccggagcctt gtagcgcccg gcccagaata
     9661 attccccaga tggagtcaga gagcgcagcg ataacgctac cggcggcaaa cactgccagc
     9721 ccaccgacaa ttaatggttt gcgaccaatg cggtcggaaa gcaggccaaa cggaatctga
     9781 aaaacggcct gagtcagacc ataaatacca atggcaatac cgattaatgc ttcgctggca
     9841 ccttgcagag ccatgccgta cgtggtcaga accggcagaa ccataaacat gcccagcatg
     9901 cgcaacgaga atacggtccc taaaccccag gtcgcgcgcc gctcacctgg cgtcatttta
     9961 taatcgttca ttaccacctc tgttttaaat tcgcgactag tgtaaagcgg caaacgaatg
    10021 gggtaaacat acggattttt tagtaaatat ttcgagaggg atatgtttct aatgctaaga
    10081 aaaaaggtgc cgcagcacct ttttaactga gaggttgtat taccacacag cagccagcag
    10141 cgtatgcgaa tccggcacca taaaatcaac ggacatcatc accgagaggg cagtgatggc
    10201 gatgatagag aagccgaaca gcttgcgcgc ccagattctg tcatcagcaa ctttataacc
    10261 gcgcagggcc atacctaacc accagacgct aaccgccgcg gcgactacca gatatttata
    10321 cccagcgtaa ccgccaagag agagcatcag cgtggcaacg gcaaaggcga tgatatacag
    10381 cgtgatgtga ttcttcgcca ccgaaatgcc ttttaccact ggcaataccg gaatgtttgc
    10441 cgcctggtaa tccttaaagc ggaaaatggc gatggcatag gagtgaggca tctgccacag
    10501 actgaagata gcaagcagga tcgctgcgcc actatcgaac tcgccggtta ctgcacagta
    10561 gccgatcacc ggcggcgcag cgccggagag cgaaccaatc aatgtgccgt agactgagtg
    10621 gcgtttcatg tacaggctat aaacgccgac gtaaaccaca aagcccatca cccccagcca
    10681 gcaggccagc ggattcgcgc caaaccacag cagcataaag ccagcaatac ccagcaaggt
    10741 ggcgtacacc agcgagacag caggagatat caggccttta accagcaccc gattcttcgt
    10801 cctttccatc tttctgtcga tatccctgtc gatgtagttg ttaaacacac aacccgacgc
    10861 cacaaccagt gacaccccaa ccagcgtgta gataaacagg ggataatcaa tgcggccctt
    10921 tgaggccagc aggaatcccc caatcaccga gatcaggttg ccaaagatga tgcctggttt
    10981 cgttacttgc aggtattgct taaacatcat aaccgccgct cttagtgcat catcatgttg
    11041 tagttaaggt tccacataat ccagatggag cctacaacca ggatagcgat gattagcacg
    11101 gtgaagacaa acgccgtcat gttccagcct tcatctgatt tggtattcat gtgcaggaag
    11161 cacaccagat gcaccagaac ctgtaccact gccattgcca ggattgttcc cagaattacg
    11221 gccggagagg cagcccctgt catcaccatc cagaacggaa tcaccgtcag aatgatcgac
    11281 aggataaagc ctgtcatgta ggtttttacg ctgccatggg acgcgccgct gtgatcggta
    11341 gaatgactca ttacatcgcc cccatcagat aaacaacagt gaacacacag atccaaacca
    11401 catccaggaa gtgccagaac aggctcaggc acatgatgcg ggtacggtta gtgctggtca
    11461 ggccgcgacg ggcgatttgc accatcaaca ccgccatcca gataagaccg gaagtgacgt
    11521 gcagaccgtg cgtgccgacc aacgcaaaga acgctgacag gaagccgctg cgatccggac
    11581 ccatgccgtt aacaatcagg tgatggaatt catagatttc catcccgata aatccggcac
    11641 caaacaacca ggtcaacgcc agccaggaga taacctggct tttgttgttt ttgtacatgg
    11701 cgatagccgc catgccgtag gtgatggagc tgaacaacag caagaaagtt tcaaccagaa
    11761 cgaacggcag ttcgaaaatg tccttacctg tcgggccgcc tgcggtgccg ttaaccagaa
    11821 cggcataggt agcaaacaag atagagaaca gaatgcagtc gctcatcagg tagatccaaa
    11881 atccgaagat tttggttccg cctgcatcgt ggtgcccgtg ttcgtgcgcg tgggcagtcg
    11941 cgtgcgtcaa agtatcagtt gccatttttc agccctgcct tagtaatctc atcgaaatgc
    12001 tggttttcca gtttttcgat ttctgccacc ggcacgtagt aatccacgtc ctcgtcgaag
    12061 cttttcacga tccaggtgat gatcatgcct gcgaagccaa caatcgccag ccaccagata
    12121 tgccagatca tggcgaaacc gaagatggtg gagaaagctg caatgacgat acctgcaccg
    12181 ctgtttttcg gcatatgaat ttcttcatag tggtcaggct ttttgtacgc ttcgcctttc
    12241 tctttcattt cccagaatgc atcacgctcg tgaacgtgcg gcacaacggc aaagttatag
    12301 aacggaggcg gggaagaggt tgcccactcc agcgtacggc caccccacgg gtcgccagtc
    12361 aggtcacggt tctggtcgcg gtcacgaata gaaacgtaca tctgaataac gaggcagaga
    12421 atacccagcg caatcagtac tgcaccgctg gctgcaatca tcagcatggt gtggaactgc
    12481 gggtcaatct gctggctcaa acgacgggtc atgcccatga agcccagcgc atacagcggc
    12541 ataaaggcaa cgaagaagcc gatgatccag aaccagaacg cgcgtttacc ccaggtttcg
    12601 ttcagtttga aaccgaacgc tttaggccac cagtaggtca tccctgcgaa gcagccgaag
    12661 accacgccgc cgatgatcac gttatggaag tgcgcaatca ggaacaggct gttatgcaga
    12721 acgaagtccg cgcccggtac tgccagcagc acgccagtca tcccgcccac cgagaaggtg
    12781 acgataaaac cgatagtcca cagcatcgca gaatggaaca cgatgcggcc ctgatacatg
    12841 gtgaacagcc agttgaagat cttcaccccg gtcgggatgg cgataatcat ggtggtgata
    12901 ccaaagaagg cgtttacgtt cgcgcccgca cccatcgtaa agaagtggtg cagccagacg
    12961 atgaacgaca gcacggtgat acagacggtt gcccatacca gcgaggtata accaaacaga
    13021 cgtttacgcg agaaggttgc cgcaatttcg gagaacacac cgaaaacagg caggatcagg
    13081 atgtaaactt ccgggtggcc ccaggcccaa atcaggttga tgtacatcat catgttgcca
    13141 cccatatcgt tggtaaagaa atgggtgccc agatagcgat ccagggtcaa caacgcgacg
    13201 gtaaccgtca gaattgggaa ggaagcaata atcagaacgt tcgcgcacag tgatgcccag
    13261 gtaaatactg gcatcttgaa catggtcatg cccggtgcgc gcatcttcag aatggtaacg
    13321 aagaagttga taccggttag cgtcgtacct ataccggata gctggagact ccatatccag
    13381 taatcgacac cgactcccgg actgtactct attcccgata gcggtggata ggccagccag
    13441 ccggtctgcg caaattcgcc cacgccgaga gaaacgttaa ccagaatcac accaacaacg
    13501 gtaaaccaga agcttaggtt gttgaggaac gggaacgcaa cgtcacgcgc gccgatctgc
    13561 agcggaacca ccaggttcat cagaccgata acgaaaggca tcgccacgaa gaagatcata
    13621 atcacgccgt gcgcggtgaa gatctgatcg tagtggtgag gtggcaggaa gcccgcttcg
    13681 cccgccgagg caagagcctg ctggctacgc atcataatgg cgtcagcaaa accacgcagc
    13741 aacatcacaa tcgccacgat gatatacatg ataccgaggc gtttatggtc gacggaggtc
    13801 agccactctt tccacaggta ggtccactta ccgaagtaag tgatcaggcc aacgagcgcc
    13861 agacctccca aaataatgcc agcgatcgta accatgacga taggttcatg gaacgggact
    13921 gcatcaagtg ataattttcc gaacatcttt attcttcctc aaccccttta atgggcggat
    13981 tccgcgtggc tcatgtccat gccttccata ccttcgtgtg cgctgtgctc accttctggc
    14041 tgggtcatgt tcatgctctt accgtgagcc ataaacttgt taatcacatc ggcaaacaag
    14101 tctggtttca cgttggagaa atattccacc tggttgtatt cgctaggcgc ggccagtttt
    14161 tcgaacgctg ccatgtcaga catggtgttc ggcgactgtt tcgcttttgc gacccactgg
    14221 tcgaatgcgg cgcgatccgg tgttgcaata gctttgaact tcatgcctga gaagcccggg
    14281 ccgctgtagc tggcggagat accgtcataa gtaccgggtt cgttggcgat cagatgcagg
    14341 cgagtctgca taccggccat ggcataaatc tggctaccca aacgcggaat gaagaaggag
    14401 ttcatcacgg agttggaggt cactttgaag tacaccggag tgttcgccgg gaaagcgatt
    14461 tcattcacgg tagcaatgcc ctgttccggg tagatgaaga accatttcca gtccatggaa
    14521 accacttcga tggtaatggg cttctcgtcg tgtgccagcg gcttgctagg ctcaagagcg
    14581 tgagtggttt tccaggtcag taccgcaagg aagatgatga ttaagatagg taccgtccag
    14641 accacagctt ccactttatt ggagtgtgac cagttcgggc tgtacttagc atctttattg
    14701 ctcgcacggt acttccaggc gaaaccaaca gccatcaaga ttgcgggaat aacgacaatc
    14761 aacatcaggc caaatgccgt cagtatcagt gaacgttgct ccagaccaat ctgtcctttg
    14821 ggatctaaca gcgcagaatt acagccactg agcaatacag tgcctgcaaa taatgacaac
    14881 catcccaaac ttttattgta tttcctgagt ctcatttaac gacctcaatt ccacgggatc
    14941 tggtggcgtt taaagtgtgt gggcatttta cgggaaggtt acattactgt aaacatgatt
    15001 aaattcctgt tacttggatt tggcttccag gtcacatatg ttgcaaaaca tatcaggttg
    15061 attttaagaa gctgttgcaa aagggcgtta taacaaaagg ggagtgca
//
LOCUS       DACBVI010000108        15086 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.108, whole genome shotgun sequence.
ACCESSION   DACBVI010000108 DACBVI010000000
VERSION     DACBVI010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.108"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            60..332
                     /locus_tag="IU325_16220"
     CDS             60..332
                     /locus_tag="IU325_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HAP5810546.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(333..1205)
                     /gene="rluF"
                     /locus_tag="IU325_16225"
     CDS             complement(333..1205)
                     /gene="rluF"
                     /locus_tag="IU325_16225"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HAP5810547.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTVGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1553..2500
                     /locus_tag="IU325_16230"
     CDS             1553..2500
                     /locus_tag="IU325_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP5810548.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQVQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIVGALRGKYINCLVTNSSTAELLLK"
     gene            2570..3376
                     /locus_tag="IU325_16235"
     CDS             2570..3376
                     /locus_tag="IU325_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP5810549.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3386..3793
                     /locus_tag="IU325_16240"
     CDS             3386..3793
                     /locus_tag="IU325_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HAP5810550.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPDENAGDIV
                     AKLEKLVSIHNHDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQSCVVWRQVATAEEDF"
     gene            3793..4287
                     /locus_tag="IU325_16245"
     CDS             3793..4287
                     /locus_tag="IU325_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP5810551.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4353..5150
                     /locus_tag="IU325_16250"
     CDS             4353..5150
                     /locus_tag="IU325_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose family PTS transporter
                     subunit IIC"
                     /protein_id="HAP5810552.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTQGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALLYIQLNPQWRKAEPQLQATTSTALDQLDD"
     gene            5161..5985
                     /gene="manZ"
                     /locus_tag="IU325_16255"
     CDS             5161..5985
                     /gene="manZ"
                     /locus_tag="IU325_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HAP5810553.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            6037..7266
                     /locus_tag="IU325_16260"
     CDS             6037..7266
                     /locus_tag="IU325_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAP5810554.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLMSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            7648..8337
                     /gene="pepE"
                     /locus_tag="IU325_16265"
     CDS             7648..8337
                     /gene="pepE"
                     /locus_tag="IU325_16265"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HAP5810555.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8427..10058)
                     /locus_tag="IU325_16270"
     CDS             complement(8427..10058)
                     /locus_tag="IU325_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HAP5810556.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10278..13961)
                     /gene="metH"
                     /locus_tag="IU325_16275"
     CDS             complement(10278..13961)
                     /gene="metH"
                     /locus_tag="IU325_16275"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP5810557.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14161..14985
                     /gene="iclR"
                     /locus_tag="IU325_16280"
     CDS             14161..14985
                     /gene="iclR"
                     /locus_tag="IU325_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HAP5810558.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3578 a         3968 c         3967 g         3573 t
ORIGIN      
        1 gttctgctcg caaacgaatt cccgtacact gaactctctg gctcactgag ggcaccagca
       61 tggcactccc ccgtattacc caaaaagaga tgactgaacg cgagcagcgt gaacttaaaa
      121 cactgctgga tcgggcgcgg attgcgcatg gtcgcgtact gaccaactcc gaaaccaaca
      181 gcattaagaa agagtacatc gataaattaa tggtcgagcg tgaggctgaa gcgaaaaaag
      241 cccgccagtt aaagaaaaag caggcttata aaccggatcc tgaagcgtca tttagctggt
      301 cagcaaatac gtcgacgcgc ggcaggcgct gattagcgcc ccttcttttt acgccccggc
      361 gaggtaaaac gcttaccgtt ggacgccggg cgaccgcctt tttccgccgt tttttccatc
      421 ttaacgactg gacgtttaat gcctacagtt ttcggtttgg ccttcgcttt aggttttgcc
      481 tcggaagagg aattttcaat gagcttaaag aggtcgatta actcatcgtc agttaaatca
      541 cgccattccc ccagcggaat gccgcttaaa ctaacgttca tgatgcgcgt acgttccagc
      601 tttttcactt cgtaaccaaa gtgctcgcac atgcgacgga tctgacggtt cagcccctgc
      661 accagggtaa tgcggaagac aaacggcgct tcttttttga ctttacactt tttcgtcacc
      721 gtcccgagga tcggcactcc cgcccccata ccacgaataa attcatcggt aatcggttta
      781 tcgaccgtca ccagatactc tttctcatga tcgttacccg cacggaggat cttattcacc
      841 agatcgccgt gattggtgag gaaaatcagc ccctgggagt ctttatccag gcggccaatc
      901 gggaacacgc gtttgctgtg gttaacgaaa tcgacaatgt tatcgcgctc gccatcttcg
      961 gtggtgctga caataccgac gggcttgttc agggcgataa gtaccaaatc ttcggcttcc
     1021 cgaggctcaa tcaactgacc gtttactttc acaacgtcgc cgggtttcac ctgatcgcca
     1081 atggtggctc gcttgccatt aaggaacaca ttgccttgct cgatatagcg atccgcttcg
     1141 cggcgtgagc aaattccgct ttcgctgatg tatttattta aacggactga tgagtcgggc
     1201 agcataaatt ctcctgtaaa acgcggaata taccgtacct cttagcgggg aaaaacaggg
     1261 ttatgcggca acgtcgaaat taaatgtgat ctgccacgcc ttttatcaca aaagtgtctc
     1321 cggaagtacg atgcaaattt gcctggcaaa ataaggataa tcaacatatt acgaaccgct
     1381 gcacacctgt gctgatgact actacgcaga gaaacaaatg tgcaagagaa agcgctattt
     1441 ctccccatgc aaagagtgaa aaatcgcttt gaaaggccat catgatgggg ttatatcaga
     1501 tcaagcggtg agaaagcgta tttgcacaaa tgtgcagaac caggaggcgg caatggaaaa
     1561 cagtgacgat atccgtttga ttgtgaagat tgcccaactc tattacgaac aggatatgac
     1621 gcaggtgcaa atcgcgcgcg aactgggtat ttaccgcacc accatcagcc gcttgcttaa
     1681 acgaggccgc gatcagggaa ttgtcaccat cgccatcaac tatgactaca acgaaaatct
     1741 ctggctggag cagcaactga agcaaaagtt tggcctgaaa gacgttgtgg tggtgtcggg
     1801 aaatgatgag gatgaagaga ctcaactggc gatgatgggg ttacacggcg cgcaactgct
     1861 ggatcgcttg ctggagcctg gcgatattgt cggtttttcc tggggtcgcg cggtgagcgc
     1921 actggttgaa aacttgccgc aggcggggca atcgcggcag ttaatctgcg taccaattat
     1981 tggcggaccg tccggtaaac tcgaaagccg ctatcacgta aacacattaa cctacagcgc
     2041 ggcagcgaag ctgaaagggg aatcgcatct cgcagatttt ccggctctgc tggataaccc
     2101 cttaattcgt aatgggatca tgcagtctca gcactttaaa accatctctg cctactggga
     2161 taatctggat gtcgccctgg tgggaattgg ctcaccggcc attcgcgacg gcgctaactg
     2221 gcatgcgttt tatggtggtg aagagagtga cgacctgaat gcccgccagg ttgctggcga
     2281 tatttgctcg cgcttttttg atattcacgg cgcaatggtt gaaacgaata tgagcgaaaa
     2341 aacactctct atcgaaatga ataaattaaa gcaggcacga tattccattg gcattgccat
     2401 gagtgaagaa aaatacagcg gaattgttgg tgcactgcgt ggaaaatata ttaattgtct
     2461 ggtaacgaat agcagcacag ctgaactatt actgaaataa aaatcttccc gatgtttacc
     2521 gtaataaaaa ttacggcggg tccctattat ctaaaaacag gagtctgtta tgcaaacgtg
     2581 gttaaatttg caggataaaa tcattatcgt caccggcggc gcatccggta taggtctggc
     2641 gattgtagag gaattattag cacaaggcgc gaatgtacag atggtcgata ttcacggtgg
     2701 cgatggacaa tatgaaggtc ataaaggcta tcagttttgg ccgaccgata tttccagcgc
     2761 caaagaggta aatcatacgg tagcagaaat tatccagcgt tttggtcgca tcgacggtct
     2821 ggttaataac gccggggtca atttcccacg tctgctggtc gatgagaaag cgcctgccgg
     2881 gcagtatgaa ctcaacgaag ctgcattcga aaaaatggtc aatatcaacc agaaaggcgt
     2941 ttttctgatg tcgcaggcgg tggcgcgaca gatggtcaaa caacacgatg gcgtgattgt
     3001 gaatgtttcc tcagaaagtg ggctggaagg ctcagaaggc cagagctgct acgccgccac
     3061 taaagctgcg ctcaatagct tcactcgttc ctggtcgaaa gagctgggta agcacggtat
     3121 ccgtgtggtc ggtatcgcgc cggggattct ggaaaaaaca ggactgcgca cgccggaata
     3181 tgaagaagcg ctggcgtgga cgcgcaatat caccgtcgag cagctgcgtg aaggctatac
     3241 caaaaacgcc attcctattg ggcgcgccgg aagattagca gaagtcgctg attttgtttg
     3301 ttatctgctg tctgaacgcg ccagctacat caccggagta accactaaca ttgcgggcgg
     3361 caaaacgcgc ggataaggag gctttatggt caacgccatt ttttgtgccc acggcaaatt
     3421 agcctgcgcc atgctggaat cggttcagat ggtttacggc aacgccaatg ttgaagccgt
     3481 tgaatttgta cccgacgaga acgccggaga cattgtggca aagctggaga agttagtgag
     3541 cattcacaat catgacgagt ggctaatcgc cgtggattta cagtgcggca gcccgtggaa
     3601 tgcggcagcg atgcttgcga tgcgcaatcc tcgcctgcgg gtaatcagcg gcctttctct
     3661 gccgctggcg ctcgaactgg ttgataacca ggacagcatg aatgttgatg aactgtgtga
     3721 acacctgacg caaatcgcaa agcaatcctg cgtcgtctgg agacaggtgg caacggcaga
     3781 ggaggatttc tgatgaacat tacgctcgcc cgcattgatg accgcctgat ccacggccag
     3841 gtcactaccg tctggtctaa agtggctaac gcccagcgca ttattatctg caatgacgaa
     3901 gtttataacg atgaagttcg ccggacatta ttgcgtcagg ctgcgccacc gggaatgaag
     3961 gttaacgtgg tcaatattga aaaagccgtc gcggtttatc acaacccgca atatcaggac
     4021 gaaaccgttt tttatctttt cactcgccca caggatgcct tagcgatggt tcgccagggg
     4081 gtaaaaatag gcaccctgaa tattggcggc atggcctggc ggcccggtaa aaaacagtta
     4141 accaaagcgg tttcattaga tgatgatgat attaacgcgt ttcatgagtt aaataatctt
     4201 ggcgtcatac tggatttacg cgtcgtagcg tcagatccct caatcaatat tatcgacaaa
     4261 ataaacgaac agttaatcgc aaattaaata atagcgcctg tgtacatatg ctttaacagg
     4321 cagggatgac gacacttaaa aggtgacaaa tcatggaaat aagtacccta caaatcattg
     4381 ctatatttct tttttcctgt attgccggaa tgggcagcgt gctggatgaa tttcagactc
     4441 atcgcccatt aattgcctgt acggtgattg gtttaattct cggtgattta aaaaccggaa
     4501 taatactcgg tggtacgctg gaattgatag ctctcggctg gatgaacgtc ggcgcggcgc
     4561 aatctccgga ttctgcactc gccagcataa tctccgccat tctggttatc gttggtcagc
     4621 aaagcatcgc caccggaatc gccatcgcgt tgcctgtggc tgcggcaggc caggtgctga
     4681 cggtgtttgc ccgtaccatc accgtagtgt tccagcacgc ggcggataaa gcagcggaag
     4741 aggcgcggtt tcgcactctc gatattctgc atgtctccgc gcttggcgtg caggcgctgc
     4801 gcgttgctat tccggcactg attgtctcgc tgttcgtcag cgccgatatg gtcagcaata
     4861 tgttaaacgc cattcccgaa tttgtgaccc aaggactaca gattgctggc ggttttatcg
     4921 tggtggtcgg ttacgccatg gtgctacgta tgatgggtgt gaaatatttg atgcctttct
     4981 ttttcctcgg tttcctcgca ggtggctacc tcgatctcag tctgctggcg ttcggtggcg
     5041 tcggcgtgat catggccctg ctctacatcc agctaaatcc acagtggcgt aaagctgaac
     5101 ctcaactcca ggccaccacc tccaccgccc ttgaccaact tgacgattaa cggagccatc
     5161 atggaacaga gaaaaattac acgcagcgat ctggtaagca tgtttctgcg ctccaacctg
     5221 caacaggcgt cctttaactt tgaacgtatt cacgggctgg gtttttgcta cgacatgatc
     5281 cccgccatca agcgacttta cccattaaaa gaggatcagg ttgcggcgct caggcgacac
     5341 ctggtgttct tcaataccac gccagccgta tgtggcccgg tcatcggcgt cactgccgcc
     5401 atggaagagg cgcgggccaa cggcgcggaa attgatgacg gtaccattaa cggcatcaaa
     5461 gtcggtctga tgggaccatt ggcaggagtt ggcgatccac tggtctgggg aacgctgcgc
     5521 ccgattaccg ccgcgctcgg cgcatctctg gcactttcgg gcaacattct cggcccgctg
     5581 ctgtttttct ttattttcaa cgcggtgcgt ctggcgatga agtggtatgg cctacaactc
     5641 ggctttcgca aaggggtgaa tatcgtcagc gatatgggcg ggaatgtgct gcaaaaactc
     5701 accgaaggcg cgtcgattct cggactgttt gtgatgggcg tgctggtcac caaatggacg
     5761 tcaatcaacg taccgttggt ggtttcacaa acgcatgccg ccgatggctc caccgtcacc
     5821 atgaccgtgc agaacattct cgaccaactt tgccctggtt tgctggcgct cggtctgacg
     5881 ctactgatgg ttcgtctgct caacaaaaaa attaacccgg tatggctgat tttcgccctg
     5941 tttggcttag ggattatcgg caatgcgctg ggcttcctgt cctgattctt cgccccggca
     6001 cgactgccgg ggccatcgct caacatgagg tggtttatga aaacaacagc tctgcgtctt
     6061 tatggtaaac gtgatttacg cctggaaacc tttgatcttc ctgaaatgca ggaggatgaa
     6121 atcctcgcga cggtggtcac cgacagcctg tgcctctctt cctggaaaga ggccaatctg
     6181 ggtgaaaacc ataaaaaagt acccgacgat gtggcgacca accccatcat catcggccac
     6241 gagttttgcg gcgatattct ggccgtgggt aaaaagtggc agcacaaatt ccagccgggt
     6301 cagcgttatg tgattcaggc caacctgcaa ctccccgacc gcccggactg ccccggctac
     6361 tccttcccgt gggtaggcgg cgaagccacg catgtggtta ttcccaacga ggtcatggaa
     6421 caagattgcc tgctggcata cgacggcgaa acctattttg aaggctcgct ggttgaaccg
     6481 ctttcctgcg tgattggcgc gttcaacgcc aactatcatc ttcaggaagg tagttataac
     6541 cacacgatgg ggattcgccc gcaagggcgc acgctgatcc tcggcggcac cggaccaatg
     6601 ggactgttgg cgattgatta tgcgctacat ggacccgtta acccgtcgct gctcgtcatt
     6661 accgataccg acaacgacaa attgagttat gcgcgcaagc actatccatc agaaccgcaa
     6721 acactgattc attatctcaa tgccgccgat gcagcatttg atacgttaat ggcactgagt
     6781 ggcggtcacg gcttcgatga tattttcgtc tttgtgccta atgaaggact ggtgactctc
     6841 gcctcttcct tgctggcgac agatggttgc ctgaatttct tcgccggacc gcaggataaa
     6901 catttcagcg cgccaattaa tttctacgat gtgcattatg catttaccca ctacgtgggc
     6961 acgtcaggcg gcaataccga tgacatgcgc gcagcagtca aattgattga agagaaaaaa
     7021 gtgcaggccg caaaagtggt aacacatatt cttgggctga atgccgcggg cgaaaccacg
     7081 cttgaattgc ctgccgtcgg cggcggcaaa aagctggtgt ataccgggaa atacctgccg
     7141 cttatgtcac tcacgcagat tcaggatcaa gcactggcgg caattctggc gcgtcatcag
     7201 gggatctggt ccggtgaggc ggagcaatat ctgctcactc atgcagaggc aatttcccat
     7261 gattaatcgc gctacttaac tctgcatgtc gctggctggt cgccgcggca acttcgcgac
     7321 cggatgtgcg gctgattgct gaagcagcac aatttgcatg agaaggataa aaaatatcag
     7381 cgccttaagg ggcgctcaaa ttggtggcaa agtacacatt gtttatgccg gatgcggaat
     7441 gaagccttat tcggcataca taatctcgaa aattcaatat attgcagtta tctggtagga
     7501 ctggtaagcg tagcgcatca ggcaatgctg tgtttgtcat cagtctcagc gcccggtgca
     7561 atacgtgaca tcgtgagcca tgtcagtaag tggttaaatt cgcagtggta gcatgcctcc
     7621 cgtgatttct tactaaccgg agaagtaatg gaactgcttt tattgagtaa ctcgacgctg
     7681 ccgggtaaag cctggctgga acatgcactg ccgctaattg ctgaacagtt gcagggtcgc
     7741 cgttcagcgg tgtttatccc tttcgctggc gtaacgcaga cctgggatga ttacacagcg
     7801 aaaacggctg cggttctcgc tccgctgggt gtttctgtca ccggtattca tagcgttgtc
     7861 gatcccgttg ccgcgattga aaatgctgag atcgtgattg tcggcggcgg gaatactttc
     7921 cagttgctga aacagtgccg cgagcgcggg ctgctggcac caattactga cgtggttaaa
     7981 cgtggcgcgc tgtatattgg ctggagcgca ggcgctaacc ttgcttgccc aaccattcgt
     8041 accaccaacg atatgccgat tgtcgatcca caaggtttcg atgcgctgaa tctgttcccg
     8101 ttgcaaatca acccgcactt caccaacgcg ctgccggaag gccataaagg tgaaacccgt
     8161 gaacagcgta ttcgcgaact gctggtcgtc gcgccagaac tcacaattat tggtctaccg
     8221 gaaggtaact ggatcacagt gagtaaaggt cacgcaacgc tgggtggccc gaacaccact
     8281 tatgtgttta aggctggtga agaagcggtg ccgctggaag cgggtcaccg tttttaagtg
     8341 aattgccgga tgcatgatcc ggcatcttgt acatagtgcc tgatgcgacg ctttacgcgt
     8401 cttatcaggc cttcaggggc gcagtattaa tactcgtccc gtccttcatc ttcatcaggc
     8461 tgttccagca cactgtaagc gactgaacaa aacagcgagt tcaggcgctg catatctccc
     8521 agtaagccta aatgtagcga actggtttca atgctctgca cgttttgctg atgcaggcga
     8581 tcgacgtgag cgtgggaata gcggcgatta agaatgcgaa aacgatgttt gctgcggcgc
     8641 aaacgacggg cgctggtaac atcgccagag aagaaaaccg acattgccag ttttaaatta
     8701 ctgagcaatt gctcatagag cgcatccagt tccttcaagc catcaagcga aaatgctcgc
     8761 cttgcagcca gcgatttgtc ggcaatttca ctacccatgc gctcgacgat atcggaggct
     8821 tgttcaaggt ttagcgacat ttcgatgatc tccgcccagc ggcgcgactc ttcttctgcc
     8881 agttcctctt ttggcattcg cgccagataa agtttaatcg cggtatagag cacgttgata
     8941 tcatccgcca gcttgcgcag ctctttctcc tgccgtggct cgccgtgcat cactttattc
     9001 aacccttcca tcatctgttc catggcgtcg ccaatgcgca gggtttcgcg cgcggcgttc
     9061 gccagagcaa gcgtgggcgt atccagcgcg ctgacatcca gatgtttagg ccgtagctgg
     9121 gtatccagtt ccggttcatc gcgaataatc gttttgcaaa accgtgccat cgggtcaaca
     9181 aatggcagca tgaccaggca acgtacaagg ttgtagaaga cgtggaaata gatcaccagt
     9241 tccgcttttg gcaatgacaa cttccccatt gtctctgcca gcaaatggac aaacggcagg
     9301 ataatcaggc tacccaccag cttaaacagc agactaccca gcgcgacacg gcgggctgcg
     9361 gcattggcgg cactgttgtt gagcatcgcc agcaggccgg aaccgaggtt agcaccaatc
     9421 accagacaga gcgccacggg gaaggagata atgcctgcag cggtcagagt cgcggtcagc
     9481 agtacagcag caaggctgga gtagctgata atggcgaaca tcgcgccaat cagcgcatcc
     9541 agcagaatat cgccggtcag cgaggcaaag atcacctgaa cgccgtttgc ctgggtgatc
     9601 ggcgttacgg cctgcacaat caactccaac gccagcaaaa tcagcccaag accaataccg
     9661 acgcggccca gttgcccggc gcgtgactgt ttgcgtccga ggaaaaaaat cacgccgata
     9721 aaaataagta atggtgacag ccaggataaa tcgaaggtga gaatacgcgc cattagcgcc
     9781 gtcccgacat ctgcacccag cacaatcacc agagccggtg cgagggctac cagatcctga
     9841 gcgacgaacg aggtcaccag catggtggtg gcattactgc tctgtaccag tgcggtaacg
     9901 ccgatccccg cgcaaaaggc gagcggcttt ttttcgacgc tccggctaag gacagtacgc
     9961 aaacgcgcgc cgaagacgcg cattacgccg gttcgaacaa tatgagtccc ccagaccagc
    10021 agggcgacgg cagaaagcag gtgaagcagc gttaacacga gttcaggttc tccttatcgt
    10081 tatatgttcc tgaagcaatt gaatgaccaa aggaccggaa acacgctgac tggcatcttt
    10141 actgtgcctt ttgaccttcg tttttccggc taagattatt ccagcctgat tccagtataa
    10201 gtgtttcata ttaataaaga gacagggcac tcattgagcg ccctgtttgt tgtaaggaaa
    10261 agtgacagat ttgtgaatca gtccgcgtca taccccagat tcggtgccag ccagcgctca
    10321 acatctgaga cgctcatacc tttacggcgg gcatagtctt caacctgatc gcgctggatt
    10381 tgcgccaccg cgtaatactt gctgtccggg tggctgaagt accaacccga aaccgatgca
    10441 ccaggccaca tggcgaaaga ttctgtgagt ttcatgccag tgtgtttttc cacttccagc
    10501 agctcccaga tggtggcttt ttccgtatgt tccgggcagg ccggatagcc cggtgccgga
    10561 cggatgccct ggtagttttc gcggatcagc tcttcgttgc tgaggttctc gttcggcgca
    10621 tagccccagt agactttacg cacacgctca tggagatact ccgcaaaggc ttcggctaaa
    10681 cggtcggcaa gcgctttcac catgattttg ttgtaatcat cgtgctgcgc ttcaaaggca
    10741 tcagccagtg cgtcctcttc cagcccgcca gtcacggcaa atgcgccgat gtaatctgct
    10801 ttaccagaaa gcttcggcgc aacgaagtca gcgagacagt agttagcgaa gccggttttt
    10861 tcggtctgtt gacgcagatg gtggctgacg ttgatcacat gggtacgcgt ttcgtcacgg
    10921 tagatttcaa tgtcatcgcc cacacggttt gccgggaaca ggcccaccac gccacgcgga
    10981 ttcagcgttt tttcggcgct taatttatcc agcatgtcgt tggcgtcttt aaacagccgc
    11041 tgcgcctcaa cgcccaccac ttcatcttcc agaatgcgcg gatacttccc ggccagcgac
    11101 caggtcataa agaacggtgt ccagtcgatg taattacgca gcgtttcgat gctggcttcg
    11161 acttcctgca cgccgagacg gtgcgccacc ggcggcgtat aagcctgcca gtcaaaagcg
    11221 aagtcgttat cacgcgccgc ttccagcgtg accggtggtg tgcgcggttt cttgcgcccg
    11281 tgctgaatac gtacggtttc gtactccttg cgggtacgag cgacaaaatc atcacgttga
    11341 gtatcggaaa gcagcgccgc caccacgcca acggtgcgcg atgcgttctg tacatacacc
    11401 gtcgggccgc tgtagttctg ctcaattttc accgccgtgt gcgcttttga ggtggtcgca
    11461 ccgccaatca gtaacggaat ggtgaagccc tgacgctcca tctctttcgc cacgttaacc
    11521 atctcgtcca gcgacggcgt gataagcccc gaaaggccaa tcaaatcagc attcacttct
    11581 ttagcggtac ggagaatttt ttccgcaggc accatcacac ccagatcgac aatttcgtag
    11641 ttgttacatt gcagcaccac gccaacgata tttttaccga tgtcgtggac atcgcctttc
    11701 acggtggcga tcaccatctt gccgttggtt ttaccctgct ctttgctggc ttcaataaac
    11761 ggttcgagat aggccaccgc ctgtttcatc acgcgcgccg atttgaccac ctgtggcagg
    11821 aacattttgc cttcgccgaa cagatcgccg accacgttca tgccgtccat caacgggcct
    11881 tcaatcactt caatcgggcg cgtagcctgc tggcgggctt cttcggtatc ctgctcgata
    11941 aactcggtaa tgcctttgac cagcgagtat tccagacgtt tattcacttc ccacgagcgc
    12001 cactccgcct gctgggcgtt ggcggtgtcg tcggttttgc tgccgcgata tttctcggca
    12061 agctccagta aacgctcagt gccatcgtcg cgacggttaa gaatcacatc ttccaccgcg
    12121 tcgcgcagtt cagcgggcaa gtcgtcgtaa atcgccagct gcccggcgtt gacgatcccc
    12181 atatccatgc cattgcgaat agcgtagtag aggaacaccg cgtgaatggc ttcgcgcacc
    12241 ggatcgttac cacggaatga gaaagaaacg ttagatacgc cgccggaaat cagcgcgtgc
    12301 ggcagttcgc gtttgatgtc ttcacatgcg ccgataaagt cctgcgcata gttgttgtgc
    12361 tcttcaatgc cggtggcgac cgcgaagatg ttcgggtcga agatgatatc ttctggcggg
    12421 aagccaacct cttcggtgag gattttgtac gcccgacggc aaatctcgat tttccgtgcg
    12481 cgagtatcgg cctgtccctg ttcgtcgaag gccattacca ccactgccgc accatagcga
    12541 cgcaacagct tcgcgtgatg gataaaggcg tcgacgcctt ctttcatcga aatagagtta
    12601 acaatgcctt tgccctggat acacttcaga cctttttcaa tgacatccca ttttgaggaa
    12661 tcgatcataa tcggcacgcg agcgatatcc ggttcaccgg caatcagatt gagaaaacgc
    12721 accatcgccg cttcggcgtc gagcatccct tcatccatat tgatatcgat aatctgcgcg
    12781 ccgttttcca cctgctggcg ggccacatcc agtgcctcgc tgtatttctc ttctttgatc
    12841 aggcgcttga acttagcgga accggtgacg ttggtgcgtt cacccacgtt cacaaacagg
    12901 ctatcttcgc caatgttcag cggctccagg ccggacaaac ggcaggctac ggggatttcc
    12961 ggcagtttgc gcggcgctaa tccttctact gcacgactca tcgcggcaat atgttgtggc
    13021 gtggtgccac agcagccgcc gacgatattg agaaagcccg cttgcgccca ttcacgtatc
    13081 tgttttgcca tcgtgtcggc gtcgagatcg tactcaccaa aggcgttggg tagcccggcg
    13141 ttcgggtgcg ctgtgacgta gcattccgca atccgtgata attcctgcac gtactggcgc
    13201 agttcgtctg gccccagcgc acagttaagg ccgaaagtca gcgcttcggc gtggcgcaga
    13261 gagttataaa acgcttcggt ggtctgcccg gagagcgtgc gcccggaggc gtcggtgatg
    13321 gtgccggaga tcataatcgg cagctcaacg cccagcgctt caaactccgt tttcaccgca
    13381 aagaccgccg ctttggcgtt aagggtgtcg aaaacggttt caatcaggat cagatccgca
    13441 ccaccttcca ccagcgcttt ggtggattcg cgataggccg ccaccagctg gtcaaaagtg
    13501 atgttgcgga atgccggatc gttgacgtcc ggagaaatag acgccgtgcg gttggtcggg
    13561 ccgagaacac cggcaacgta gcgcggtttc tccggcgtgc gcgcggtcca ctcatcagca
    13621 caggcgcgcg ccagtttcgc cgccgcaaag ttgatttccg ccgacaggga ttccatctgg
    13681 taatccgcca tcgcaatggt tgtggagttg aaggtgttgg tttcgatgat atccgcgccc
    13741 gcttcaaagt aggcgttgtg gatagcggcg atcacttccg gtttactgag taccagcagg
    13801 tcgttgttgc ctttgaggtc gcatggccag tcggcaaagc gttcaccacg aaaatcggct
    13861 tcgttcagtc gatagctctg gatcatggtg cccataccgc cgtccagcac cagaatacgt
    13921 tcatttaact gcgcacgcag ttgttccact ttgctgctca cacttgctcc cgacacgctc
    13981 aacccagatt taataaaaat tcaacaaacc atactggcat aaacgcatct gtggtaaaag
    14041 cgaccaccac gcaacatgag atttgttcaa cattaactca tcggatcagt tcagcaacta
    14101 ttgcattagc taacaataaa aatgaaaatg atttccacga tacagaaaaa ggagactgtc
    14161 atggtcgcac ccattcccgc gaaacgcggc agaaaacccg ccgttgccac cgcaccagcg
    14221 actggacagg ttcagtcttt aacgcgtggc ctgaaattac tggagtggat tgccgaatcc
    14281 aatggcagtg tggcactcac ggagctggcg caacaagccg ggttacccaa ttccacgacc
    14341 caccgcctgc taaccacgat gcaacagcag ggctttgtgc gtcaggttgg cgaacttgga
    14401 cactgggcga tcggcgcgca tgcttttatg gtcggtagca gctttcttca gagtcgtaat
    14461 ctgttagcca ttgtccatcc tattctgcgc aatctgatgg aagagtccgg cgaaaccgtg
    14521 aatatggcgg tgctggatca aagcgatcat gaagcgatta ttatcgatca ggtacaatgt
    14581 actcacctga tgcgtatgtc cgcgcctatc ggcggtaaat taccgatgca tgcttccggt
    14641 gcgggtaaag cctttttagc ccaattaagt gaagagcagg tgacgaagtt actgcatcgc
    14701 aaaggcttac atgcctatac ccacgcgacg ctggtgtctc ctgtgcattt aaaagaagat
    14761 ctcgcccaaa cgcgcaaacg gggttattca tttgatgatg aagaacatgc cctgggactg
    14821 cgttgtctcg cggcatgtat tttcgatgag caccgagaac cgtttgccgc aatttcaatt
    14881 tccggaccga tttcacgtat taccgatgac cgcgtgaccg agtttggcgc gatggtgatt
    14941 aaagcggcga aggaagtgac gctggcgtac ggtggaatgc gctgactttt tctggcgggc
    15001 agaggcaata ttctgcccgt catacccgac tggcaataga ataaggggtc tgttaatcgc
    15061 attgacgcca aaataactta ttgtca
//
LOCUS       DACBVI010000109        15051 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.109, whole genome shotgun sequence.
ACCESSION   DACBVI010000109 DACBVI010000000
VERSION     DACBVI010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.109"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(44..2323)
                     /locus_tag="IU325_16285"
     CDS             complement(44..2323)
                     /locus_tag="IU325_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HAP5810559.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRSDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2612..3562
                     /gene="tal"
                     /locus_tag="IU325_16290"
     CDS             2612..3562
                     /gene="tal"
                     /locus_tag="IU325_16290"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAP5810560.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGRKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3582..5585
                     /gene="tkt"
                     /locus_tag="IU325_16295"
     CDS             3582..5585
                     /gene="tkt"
                     /locus_tag="IU325_16295"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP5810561.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKVHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5688..6731)
                     /locus_tag="IU325_16300"
     CDS             complement(5688..6731)
                     /locus_tag="IU325_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HAP5810562.1"
                     /translation="MRYRIFLLFFFALLQTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLNGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNSGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6857..7432)
                     /gene="nudK"
                     /locus_tag="IU325_16305"
     CDS             complement(6857..7432)
                     /gene="nudK"
                     /locus_tag="IU325_16305"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HAP5810563.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDSQRANAGGGVEDEDIEVLELPF
                     NKALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7500..9479)
                     /gene="aegA"
                     /locus_tag="IU325_16310"
     CDS             complement(7500..9479)
                     /gene="aegA"
                     /locus_tag="IU325_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HAP5810564.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9685..11385
                     /gene="narQ"
                     /locus_tag="IU325_16315"
     CDS             9685..11385
                     /gene="narQ"
                     /locus_tag="IU325_16315"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HAP5810565.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11549..14662
                     /gene="acrD"
                     /locus_tag="IU325_16320"
     CDS             11549..14662
                     /gene="acrD"
                     /locus_tag="IU325_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HAP5810566.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14761..14820)
                     /gene="ypfM"
                     /locus_tag="IU325_16325"
     CDS             complement(14761..14820)
                     /gene="ypfM"
                     /locus_tag="IU325_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HAP5810567.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3524 a         3992 c         3937 g         3598 t
ORIGIN      
        1 cttaccctga atattcaggg taagcgtgag agttaaaaaa gaattacagc ggttgggttt
       61 gcgcttcaac cacggccagc gccaccatgt tgacgatacg acgtaccgat gcgatcggcg
      121 ttaacacgtg aaccggtttc gccacgccca tcagcaccgg accgacagtc acaccttccg
      181 agctggaaac acgcagtaag ttgtaactaa tgcgggcagc ttccatgttc ggcatcacca
      241 gaatattagc ggaacctttc aaagggctgt ccggcatacg gtcgctgcga atcgcttcca
      301 ccagcgctgc atcgccgtgc atttcaccat caatcatcag ttctggtgca cgttccctga
      361 ccagttccag cgcctgacgc attttgctcg acgacgggca gtcagaagaa ccaaagttgg
      421 agtgagacaa cagagccaca cgcggctcaa taccaaaacg acgaacagtt tctgccgcca
      481 tcaaggtgat ctccgcaagc tcttctgcgt ctggttcgtc attgacgtag gtatcggcaa
      541 taaaggtgtt accactcggc agcagcagcg cgttcatggc acctgcggtg tgaacgccat
      601 cgcgataacc aaagacattt ttcaccacgc taaaatgttc atgataatcg cccaccgtac
      661 cgcagatcat tgcatcggct tcgccacgct gaaccatgat cgcgccaatc actgtcgggt
      721 tactgatcaa tgcacgctgc gcctgttcct gagtgacacc gcgacgcttc atgatctgga
      781 agtattcggt ccagtactct ttaaagcgcg gatcggattc gttattgacg atctcaaaat
      841 caacacccgc tttgatctgc aagcccagtt tctgaatgcg catttcgatc acgttcggac
      901 gaccgataag gatcggtttc gccagcccca gcgttaccag ttcctgagtg gcatgcagaa
      961 cgcgcgcctc ttcaccttcc ggcagaacga cgcgcttcgg cgctttgcga gcctgggaaa
     1021 aaatcggctt cataaacagg ttggttttgt aaacgaactc cgtcagcttg tcgatgtaga
     1081 cgtcgaaatc agcaatcgga cgcgtcgcca cgccggactc catcgctgct ttagcgaccg
     1141 caggagcgat cttaacgatc aagcgcggat caaacggttt tggaatgatg tattccggac
     1201 caaagctcag atcctgatcg ccataagctg aagccaccac ttcactctgt tccgcatggg
     1261 ccagttctgc aatcgcgcgt accgccgcca gtttcatctc ttcgttgatg gcggttgcgc
     1321 caacgtccag cgcgccacgg aagatgaacg ggaagcaaag aacgttgttc acctggttcg
     1381 ggtagtcgga acggccggta cagatgatgg catccggacg cacttctttc gccagcggcg
     1441 gcagaatttc cggttccggg ttcgccagcg ccaggatcat tggcgcacgg gccattttct
     1501 tcaccatttc ctgggtcagc actttcgggc cggaacagcc gaggaaaata tccgcacctt
     1561 caatcacatc atcgagggta cgtttgccgt catccaccac cgcatatgcg gctttggttt
     1621 ccgccatgtt tggctcacgg ccctgataga taacgccttt tgagtcgcaa accacgatgt
     1681 tatgtttttg cagacccagc gctaccagca ggttcataca ggcgattgct gcggcacccg
     1741 cgccggaaac caccatccgc acgtcggaga tgtttttctc caccacgcgc aaaccgttga
     1801 ggatggcggc agtgctgatg attgccgtgc cgtgctgatc gtcgtggaat accggaatat
     1861 tcatccgctc gcgcagtttc tgttcaatat agaaacattc tggcgcttta atgtcttcga
     1921 ggttgatgcc gccgaaggtc ggttcaagcg cggcgacgac ttcaataaat ttgtccgggt
     1981 cgagttcatc aacttcaatg tcaaatacat caatcccggc gaatttctta aacagaacgc
     2041 ccttgccttc catcaccggt ttgcctgcca gcgcgccaat gttgcctaac cccagcactg
     2101 ccgtaccgtt agagatcacc gccaccaggt taccacgcgc ggtatatttg taggctttta
     2161 acgggtcttt ttcgatttca agacaaggtg cggcaacgcc tggtgagtag gccagcgcca
     2221 gatcgcgctg cgttgccaga ggcttggttg gagaaacctg gattttccct ggaactggaa
     2281 attcatggaa atcaagtgca ctttgtttta actggtcatc catttggttg ttcctttcac
     2341 gtaacgttca caaataaagt gtgtgggcaa cagcccctgc ccacaacgtg gcgcacatta
     2401 ttaccttgcc ggagtctaca gactttgagc aagtccaaac tctcaccatt aatataatgt
     2461 tttggtaata atcctataac actgatgtta cctgcttaat ccagcaatac catgcctgtc
     2521 tgctatgctt ttttgatgcg tttagcgaaa tttctcagaa gtgtgaatta acgcactcat
     2581 ctaacacttt acttttcaag gagtatttcc tatgaacgag ttagacggca tcaaacagtt
     2641 caccactgtc gtggcagaca gcggcgatat tgagtccatt cgccattatc atccccagga
     2701 tgccaccacc aatccttcgc tgttactcaa ggctgccgga ttatcacaat atgagcattt
     2761 aatagacgat gctatcgcct ggggtagaaa aaatggcaaa acccaggaac aacaggtggt
     2821 cgcagcgtgt gacaaactgg cggtcaattt cggtgctgaa attctcaaaa tcgtacccgg
     2881 tcgcgtgtca acagaagttg atgcacgcct ctcttttgat aaagaaaaga gtattgagaa
     2941 agcgcgccat ctggtggact tgtatcagca acaaggcgtt gagaaatcac gtattttgat
     3001 caagctggct tcaacctggg aaggaattcg cgcggcagaa gagctggaaa aagaaggtat
     3061 taactgcaac ctgacactgc tgttttcttt tgcacaggcg cgggcctgtg cagaagcagg
     3121 cgtatttcta atttcgccgt ttgtcgggcg tatttatgac tggtatcagg cacgcaagcc
     3181 gatggacccg tatgtggtgg aagaagatcc aggcgttaaa tcagtacgca atatctacga
     3241 ctactataag caacaccact atgaaaccat tgtgatgggc gcgagcttcc gtcgcaccga
     3301 acaaatcctc gccttaaccg gctgcgatcg actgactatc gcaccgaatt tgctgaagga
     3361 gctgcaggaa aaagtttcgc cagtggtacg taaattaatc ccaccttctc agacgttccc
     3421 acgccccgct cccatgagcg aagcggagtt ccgttgggag cacaatcagg atgcgatggc
     3481 ggtagaaaaa ctgtctgaag gcattcgtct gttcgccgtt gatcaacgca aactggaaga
     3541 tcttcttgcc gccaaactat aaaccagcca cggagtgtta tatgtcccga aaagaccttg
     3601 ccaatgcgat tcgcgcactc agtatggatg cggtacaaaa agccaattct ggtcatcccg
     3661 gcgcgccgat gggcatggct gatattgctg aagtgctgtg gaacgatttt cttaaacata
     3721 accccaccga cccaacctgg tatgatcgcg accgctttat tctttccaac ggtcacgcgt
     3781 cgatgctgct ctacagtttg ctgcatctga ccggttacga cctgccgctg gaagagctga
     3841 agaacttccg tcagttgcat tcaaaaaccc caggtcaccc ggagattggt tatacgccag
     3901 gtgttgaaac caccaccggt ccacttggac aaggtttggc gaacgccgtc ggactggcga
     3961 tagcagaacg tacgctggcg gcgcagttta accagccgga tcatgaaatt gtcgatcact
     4021 tcacctatgt gtttatgggc gacggctgcc tgatggaagg tatttcccac gaagtctgtt
     4081 cgctggcggg cacgctggga ctgggcaagc tgattggttt ttacgatcac aacggtattt
     4141 ccatcgacgg tgaaaccgaa ggctggttta ccgacgatac ggcaaaacgt tttgaagcct
     4201 atcactggca tgtgatccac gagatcgacg gtcacgatcc gcaggcggtg aaggaagcga
     4261 tccttgaagc gcaaagcgtg aaagataagc cgtcgctgat tatctgccgt acggtgattg
     4321 gctttggatc gccgaataaa gcaggtaagg aagaggcgca cggcgcacca ctgggggaag
     4381 aagaagtggc gctggcacgg caaaaactgg gctggcacca tccgccattt gaaatcccta
     4441 aagagattta tcacgcctgg gatgcccgcg aaaaaggcga aaaagcgcag cagagctgga
     4501 atgagaagtt tgccgcctat aaaaaggtcc atccgcaact ggcagaagag tttacccgtc
     4561 ggatgagcgg cggtttaccg aaggactggg agaaaacgac tcagaaatat atcaatgagt
     4621 tgcaggcgaa tccggcgaaa atcgccaccc gtaaggcttc gcaaaatacg cttaacgctt
     4681 atggcccctt gctaccggag ctgctcggcg gttcggcaga tctggctccc agcaacctca
     4741 ccatctggaa aggttctgtg tcgctgaagg aagatccggc gggcaactac attcactacg
     4801 gggtgcgtga atttggcatg accgctatcg ccaacggcat tgcgcaccac ggcggctttg
     4861 tgccgtatac cgcgacgttc ctgatgtttg ttgaatacgc ccgtaacgcc gcgcggatgg
     4921 cggcactgat gaaagcgcgg cagattatgg tttataccca cgactcaatt ggtctgggcg
     4981 aagatggtcc tacgcaccag gctgttgagc aactggccag tctgcgctta acgccaaatt
     5041 tcagcacctg gcgaccgtgc gatcaggtgg aagcggcggt gggctggaag ctggcggttg
     5101 agcgccacaa cggaccgacg gcactgatcc tctccaggca gaatctggcc caggtggaac
     5161 gtacgccgga tcaggttaaa gagattgctc gtggtggtta tgtgttgaaa gacagcggcg
     5221 gcaagccgga tattattctg attgccaccg gttcagagat ggaaatcacc ctgcaagcgg
     5281 cagagaaatt agcaggagaa ggtcgcaatg tacgcgtagt ttcgctgccc tcgaccgata
     5341 ttttcgacgc ccaggatgag gaatatcggg agtcggtgtt gccttctaac gttgcggctc
     5401 gcgtggcggt ggaagcaggc atcgccgatt actggtacaa atatgttggt ctgaaagggg
     5461 caattgtcgg gatgaccggc tatggggaat ctgctccggc agataagctg ttcccgttct
     5521 ttggctttac cgccgagaat attgtggcaa aagcgcataa ggtgctggga gtgaaaggtg
     5581 cctgatggtg atcgccggat gctatatgtt cccctcaccc taaccctctc cccagagggg
     5641 cgaggggacg gtccgtgcgc tttttagggg cgcatctggg attttgttta acgggtgatc
     5701 cacaacgtgg gccaggcgtc tggcccatgc cagttatcgc aggtgggttc tgcggcgtaa
     5761 cgcaccaggc gaaaacgctg accgtcaaag cgccagcgcg cctgaatgcc acaatcgctt
     5821 aatccgcgcc ctttcgctaa ggtcaccagt tcacgcgatt tttcatcgaa tgttgcgttc
     5881 atcagttcca gttcattcgt ctcctgaccg ctgttgaacg gcaaacgtaa ccgaaccggg
     5941 cgcgaggcca gtggcttttt acgcgacaca atccaggcca aatcaatggt gttataggcc
     6001 cctgcctcac agctaatcat catcagcgct ttatcatcgg tcagcgcagt gacatttacc
     6061 tctcgacgca atggatcaag cgagcagcgc agaccattca tccgccagtt tccataatcc
     6121 agcaaatcgt tgcgttcttc gagtgagagt ggcgtcggcg ttgggttaac caccgcgacc
     6181 tctttcagcg caggcgcggg cggtacgctg agcggcggtt cgtccccttt ctttatccac
     6241 gcggtttcac tgccaacgcg cttttgctga gcatcaataa acaacaacgc cgcttttaag
     6301 ccattcagag aaatggtctg attgccatcg cgtaaggtga ttgccttccc ttcctgaatc
     6361 atctgcaaaa acgcggtgat ggttgccgta tcatcggtta ctaataacca tggtgaaatc
     6421 cgccacttgt caccacttaa cgccaacggc tcgccatcta acagcagccg tggcgctatc
     6481 tccccttcag acgcatccgg cgacttcaac ccgccgcgct caatacgtaa aacggcatcc
     6541 gtatgcgccc cggcgctgcg gctcagggtc atcaccagtc cgttatgatc gcccgtatta
     6601 cgcgccacgc agaagttttg gttattacag gtgacctgcc agtcggaaaa cgcccgttgc
     6661 gctggtgccg cccacaccaa agacgtctgc aacaaagcga aaaaaaagag aaggaaaatg
     6721 cgatagcgca tggacggtac gaccccagaa gttcaaacaa acagcacaag tcgtatcttc
     6781 gttgcgcagg tagcgcggct caatcggatt tatcggattg agccaataat aaacaacgaa
     6841 atattattgt tatttttcag tccattaaat gtgacatttg caaatagtta agcaataaca
     6901 ccgtcttacc gtcacgtatc tcgccagttt tgatcatctc cagcgccttg ttgaacggca
     6961 gctcgagcac ttcaatatct tcatcttcga caccaccgcc ggcgttagcg cgctggctgt
     7021 cactgtattc ggcgataaaa aagtggatta gctcagtcac accgccaggc gacatataca
     7081 gttcaaataa tttgcgcact tcgcccactt catagcccgt ctcttcaatc gcttctttgc
     7141 gaatgcacac ttccggttcg tcgttatcca gcagcccggc gcaggtttca atcagctgcc
     7201 cgctttcatt gccattaacc caggtagcga cgcggaactg acgaatcaga actacagtct
     7261 ttttcttcgc gttgtacagg aggatcgttg caccattacc gcgatcgtaa acttcacgtt
     7321 tatgacggat aacttcgcca tctttgcgcg tgagatcgta agtaatgttg tgcagggtga
     7381 aatagttatc ggagagaatt ttgtctttaa tgagggtgat ttgttgcgtc ataccgactc
     7441 cacagcgcga aatgaacaat tatcttacgc tgtgaagccg ggtttgtctg cgcaggctat
     7501 cagtgagatt tgactgattt tacccccagc cagtcaataa tcccctgtgc cgcatgacgt
     7561 ccttctgcca ttgcggtaac caccagatcc gcaccacgca cggcgtcacc accggcgaag
     7621 attttcggat tcgtggtctg ataacggtac tggctttcca catccgcgat gatgcggccc
     7681 catttgtcta ccgttacacc gtgcgactcc agccacggca tcccgtgcgg attgaagcca
     7741 aacgccataa tcaccgcgtc ggctggcatg acaaattcac tgccttccac cggtactgga
     7801 cgccgacgcc cctgggcatc cggctcgcca agacgcgtac gcaggaaacg aatcccgcaa
     7861 acgtgacctt gttcattcag ctcaagcgcc accggctgga cgttaaattc gaagttggcc
     7921 ccctcttcgc gggcgttctt cacctctttc ttcgagcctg gcatgttagc ttcatcacga
     7981 cgataagcgc aggtaacgtt actcgcgccg tggcgcagcg cagtacgcac acagtccatc
     8041 gcggtgtcgc cgccgcccag taccacgacg ttgagtccgg cggtattgat aaacggctct
     8101 tccggtagct cttcgagtcc catcacctgt ttagtgttgg cgatgaggaa cggcagggcg
     8161 tcataaacgc ccggcgcatc ttcattgggt aaacccgctt tcatggaacg gtaagtgcct
     8221 acgccaacga agaccgcgtc gtattgttcc aaaagcgaat ccaaagagac atctttaccc
     8281 acttcacaat tgagttcgaa gtgaatcccc atcgcgctga agatttcccg acggcgtgcc
     8341 agcagggatt tatccagttt gaaagaagga atgccgaaag tgagcaagcc accaatttct
     8401 ggatggcgat cgtacaccgt caccgccacg ccattgcggg tcagaacatc cgcacaggcc
     8461 agccctgccg gacctgcacc gataatcgcc acccgcttgt ccactttggt gacatggctt
     8521 aagtcaggac gccaaccttt cgccaacgcc tgatctgaaa tgtagcgttc aatgttgccg
     8581 atagttactg caccgtgctc atcgcgaata gtacaggctc cttcacacaa acggtcttgc
     8641 ggacaaacgc gtccggtaat ttccggcagg gtgttggtct ggtgagaaag ctcgacggcg
     8701 gcgtcgatgt ttccggcttt caccagttca atccactgcg gaatatggtt atgcagcggg
     8761 caggtccatt cacaaacgct gtgctcgccg cacttaaggc agcgcgaggc ttcccgttgt
     8821 gcctgatcgg cacggaatgg cagataaatt tcatcaaaac cggttttacg cgcttcaatc
     8881 gccagtttgt ccggctcgcc acgcgcgggc gttgcctgca tttgctcgac tttactcatt
     8941 accggcattt cttgcgccgc ggtactggca tgccacggtt gatgttcctg acgtgcggta
     9001 cgcaagcggc gggatttcgc catgcctgag agtgcggcgt cagtgaccag ttgcagtgcg
     9061 tccgctgggc aattctcgac acaagcagga ccgttttcgc gccctgcaca aaggtcgcat
     9121 ttatgcgccg tggcttttac ttttcctgcc gcgacggggg taaggacgat ttgcatcgta
     9181 ccaaatggac aggccaccac gcaggattta cagccaatac acttttgctg attgacctga
     9241 atgctgtcat caacgtggct gattgcgcca ttagggcagc tacgggcgca gggcgcatct
     9301 tcgcaatggt gacaggtcac tgcactacgt tgctgctgat gtttgataac cgtaattcgg
     9361 ggatgaaaat ggtgttggct caggacatgt tgctcatcat tgtgagccat gacacaggcg
     9421 atttcacaag catgacaacc cagacattgc tgactgttgg ccataataaa acgattcata
     9481 acgaccttct tttttggttg taaaaacctt attctttata tgagtgttgt tattacccga
     9541 cttacagggg aatcggcaat gttcatgtgc ccagaataag taactatttc gctataaact
     9601 gtggcagatc aaataatccc atcactgact aaattgcgtt tcagcgaact ggaacattaa
     9661 tgattttttg tggagaagac gcgtgtgatt gttaaacgac ccgtctcggc cagtctggcc
     9721 cgggcctttt tttacattgt cctgctgtcg attctttcca cgggtatcgc tctgctaact
     9781 ctggcgagca gtttgcgcga cgctgaggct atcaatattg ccggatcgct gcgtatgcag
     9841 agttaccgcc tgggctacga tttgcaaagt ggcagtccac aactcaatgc acatcgccag
     9901 ttatttcagc aggcactgca ttcaccggta ttaaccaatc tcaacgtctg gtatgtgcca
     9961 gaggcagtaa aaacccgcta tgcgcatctg aatgccaact ggctggagat gaataatcgg
    10021 ctcagcaagg gcgacttgcc gtggtatcag gccaatatta ataattatgt taatcagata
    10081 gacctgttcg tactggcttt acagcactac gctgaacgca aaatgctgct ggtggtggcg
    10141 atttccctgg ctggcggcat cggtattttc acgctggtct tttttacttt gcgccgcata
    10201 cgccatcagg tggttgcccc gctgaatcag ctggttaccg ccagtcagcg tattgaacac
    10261 gggcagttcg actcgccgcc gctggatacc agcctgccga atgagcttgg cctgcttgcc
    10321 aaaaccttta accagatgtc gagcgagctg cataaattgt accgttcgct ggaggcgtca
    10381 gtagaagaaa agacccgcga cctccacgag gccaagcgtc gcctggaggt gttgtatcag
    10441 tgttcgcagg cgctgaacac cagccagatt gatgtgcatt gtttccgcca tattttgcag
    10501 attgttcgcg acaacgaagc ggctgaatat ctggagttaa atgtcggtga caactggcgg
    10561 attagcgaag ggcagccaaa cccggaattg ccgatgcaga ttttaccggt gacaatgcaa
    10621 gagacggttt acggcgaact gcactggcaa aatagtcacg tttcatcatc agaaccgctg
    10681 cttaacagcg tttcgtcgat gctgggacgc ggtttgtact ttaatcaggc gcagaagcat
    10741 tttcagcagt tattgttgat ggaagaacgt gcgaccatcg cccgcgaatt gcacgactcg
    10801 ctggctcagg tactttctta cttacgtatc cagttgacgt tactgaagcg ttcgataccg
    10861 gaagataacg ccaccgcaca aagtatcatg gccgattttt cccaggcgtt gaatgatgct
    10921 tatcggcagt tacgcgagct gttaactact ttccgcctga cgctgcagca ggcggatctc
    10981 ccctctgcgc tacgtgaaat gctggatacg ttacaaaatc aaaccagcgc caaactgacc
    11041 ctcgactgcc gtctgccaac cctggcgctg gatgcgcaaa tgcaggtgca tttgttgcaa
    11101 attattcgcg aagcggtgct gaatgcgatg aagcacgcca acgccagcga aatcgccgtt
    11161 agctgcgtca ccgcgccgga tggcaatcac acagtctata ttcgcgacaa cgggattggc
    11221 atcggtgaac cgaaagaacc cgaaggtcat tatggcctga atatcatgcg cgagcgcgcg
    11281 gaacggctgg gtgggacgct gactttttca caaccttccg gcggcggcac gttagtgagt
    11341 attagctttc gctccgcgga gggtgaggaa agtcagctga tgtaatgcct ccgactgacc
    11401 aaagaatact ggcccttaaa gttcagtata aaagggcatg ataatttaca ttaactcctt
    11461 tttttctcca cgattggctc gtaccttgcc gctacagtga agcaagtcaa gcctacaacg
    11521 atacgcagaa acacgaggtc ctcttttaat ggcgaatttc tttattgatc gccccatttt
    11581 tgcctgggtg ctggcaatcc tgttgtgtct gacagggacc ctggcgattt tctcattgcc
    11641 cgttgaacaa taccccgatc tcgcgccacc gaatgtgcga gtgaccgcta actatcccgg
    11701 cgcatcggcc cagacgctgg aaaacaccgt gacccaggtt atcgagcaaa atatgaccgg
    11761 cctcgataat ctcatgtata tgtcgtctca gagcagtggc accggccagg catctgtcac
    11821 gttaagtttt aaagcaggca ccgatccgga cgaagccgtg cagcaagtac aaaaccagct
    11881 gcaatcagcc atgcgaaagt taccgcaggc ggtgcaaaat cagggcgtga cggtgcgtaa
    11941 aaccggcgat accaatattc tgaccattgc cttcgtctct accgatggtt cgatggataa
    12001 acaggatatt gccgattatg ttgccagtaa tattcaggac ccgttaagcc gcgtgaatgg
    12061 cgtcggggat atcgatgcct atggttcgca atattccatg cgtatctggc tggacccggc
    12121 gaaactcaac agtttccaga tgacggctaa agatgtcact gatgccattg agtcacagaa
    12181 cgcgcagatt gcggttgggc aacttggtgg tacgccttcc gtcgataagc aggcgctcaa
    12241 cgccaccatt aacgcccagt cactgctgca aacaccagaa cagttccgcg atatcacctt
    12301 gcgggtgaat caggacggct cagaggtaag gctgggcgat gtcgccaccg tcgaaatggg
    12361 ggcggagaaa tacgattatc ttagccgctt caatggtaaa ccagcctccg ggttgggggt
    12421 aaaactggcc tccggcgcga acgaaatggc gacagcggag ctggtgctca atcgtcttga
    12481 cgagctggcg cagtatttcc cgcatggact ggaatacaag gtggcgtatg aaaccacctc
    12541 gtttgttaaa gcctccattg aagacgtggt gaaaacgctg ctggaagcta tcgctctggt
    12601 tttcctcgtt atgtatctgt tcctgcaaaa cttccgcgcc acgctgatac ccactatcgc
    12661 cgtgccggtg gtgttgatgg gaaccttctc cgtactttat gccttcggtt acagcgtcaa
    12721 caccttaacc atgttcgcga tggtgctggc gattggtctg ctggtggatg acgccatcgt
    12781 ggtggtggaa aacgtcgaac gtattatgag tgaggaagga ctcactcctc gcgaagccac
    12841 acgtaaatcg atggggcaga tccagggggc actggtcggg attgcgatgg tgctttcggc
    12901 ggtatttgta ccaatggcct tcttcggcgg caccaccggt gccatctatc gccagttctc
    12961 tattaccatt gttgcggcga tggtgctgtc agtattggta gcgatgatcc tcactccggc
    13021 tctgtgtgcc acgctactta agccactgaa aaagggtgag catcacgggc aaaaaggctt
    13081 ttttgcctgg tttaaccaga tgtttaaccg caacgccgaa cgctacgaaa aaggggtggc
    13141 gaaaattctc caccgtagcc tgcgctggat tgtgatttat gtcctgctgc ttggcggcat
    13201 ggtgttcctg ttcctgcgtt tgccgacgtc gttcttgcca ctggaagacc ggggcatgtt
    13261 tactacctcg gtacagttgc ccagcggttc aacccaacaa cagaccctga aagtcgttga
    13321 gcaaatcgag aaatattact tcacccatga aaaagacaac atcatgtcgg tgtttgccac
    13381 tgttggttct ggccctgggg gtaacgggca aaacgtggcg agaatgttta tccgcctgaa
    13441 agactggagc gaacgcgaca gtaagaccgg cacctcgttt gccattatcg agcgtgcaac
    13501 gaaggcgttt aacaagatta aagaagctcg cgttatcgcc agcagcccgc cagcaattag
    13561 cggtcttggt agttctgcgg gttttgatat ggagttgcag gaccacgctg gagcaggtca
    13621 cgatgcgctg atggcagcac gtaaccagtt gctggcgctg gcggcggaaa acccggagct
    13681 aacccgtgtg cgccataacg gcctcgacga cagtccgcag ttgcagattg atatcgacca
    13741 gcgtaaagct caggcgctgg gcgttgctat cgacgatatt aacgacacac tgcaaaccgc
    13801 ctggggttcg agctatgtga atgactttat ggatcgcggt cgcgtgaaga aagtctatgt
    13861 gcaggcagct gcgccgtatc gcatgctgcc agatgatatc aatctctggt atgtccgaaa
    13921 taaagatggc ggcatggtgc ccttctctgc tttcgcgacc tcacgctggg agacaggctc
    13981 gccgcgtctg gaacgctata acggttattc tgcggttgag attgttgggg aagccgcacc
    14041 gggggtcagt accggtacgg cgatggatat tatggaatcg ttagtgaagc agttgccaaa
    14101 cggctttggt ctggagtgga cggcgatgtc gtatcaggag cggctttccg gcgcgcaggc
    14161 tccggcgctg tacgccattt ccttgctggt ggtattcctg tgtctggcgg cgctgtatga
    14221 aagctggtcg gtgccgttct cggtaatgct ggtcgtgccg ctgggggtta tcggcgcgct
    14281 gctggcaacc tggatgcgcg ggctggaaaa cgacgtttac ttccaggtgg gcctgttaac
    14341 ggtcattggt ttatcggcga aaaacgccat cctgatcgtc gagtttgcta acgagatgaa
    14401 ccaaaaaggc cacgacctgt ttgaagcgac gctccacgcc tgccgtcagc gtttacgccc
    14461 gattctgatg acctcgctgg catttatctt cggcgtattg ccaatggcaa ccagcacggg
    14521 tgccggttcc ggtggtcagc atgcggtggg tactggcgta atgggcggga tgatttcggc
    14581 cactattctg gctatttact tcgtgccgct gttctttgtg ctggtgcgcc gccgcttccc
    14641 gctgaagccg cgcccggaat aagcaataaa aaaggcgaca tgccaatgtg tcgccttttt
    14701 caactttccg attaagaacc tgctcagcag gttcttgctg tttgtacttt gtctcaggaa
    14761 ttacttacga agcataactt cgataaagtc tttccagttc cccagttcac gttcaatcat
    14821 aacaacctct cttataatta tgggtattct acggaaacaa tataccgtgg tgaagctaat
    14881 ttactcgatt gctgcgatga ctacctccgg gggacaaacc ttatgtaaat actatggtcc
    14941 tgcggtgatg atttgtatgt gatacacagc aacatttcga gatattcata cggcatctaa
    15001 tacttattta attctggtta aaatacagac agataacaag atgaatattc t
//
LOCUS       DACBVI010000110        14763 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.110, whole genome shotgun sequence.
ACCESSION   DACBVI010000110 DACBVI010000000
VERSION     DACBVI010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.110"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            221..787
                     /locus_tag="IU325_16330"
     CDS             221..787
                     /locus_tag="IU325_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP5810568.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSNNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            838..3297
                     /locus_tag="IU325_16335"
     CDS             838..3297
                     /locus_tag="IU325_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAP5810569.1"
                     /translation="MDTVNIYRLSFISCLVMAIPSALAVEFNLNVLDKSMRDRIDISL
                     LKEKGGIAPGEYFVSVAVNNNQISNGQKIDWKKNGDQTIPCINDLLVDKFGLKPEVRQ
                     SLPRLNQCVDFSSRPEILFIFDQASQQLNITIPQAWLAWHSDNWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSNSTNLNAYGTAGINAGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTVSHSGRVIYQKKVLPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRTSMSHQTENETFFSNEVSWGMLSNTSLYGGLLISGDN
                     YHSAAMGIGQNMLWLGALSFDVTWASSQFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDNDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGNWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSNHSTTHRMSWNDTLDERNSWGMSTGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVADNVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQLPPLGADIRQEDSGISVGIVGEEGHAWLSGVAENQQFTVIWGDSQR
                     CSIHLPEHMEDTANRLILPCH"
     gene            3312..4043
                     /locus_tag="IU325_16340"
     CDS             3312..4043
                     /locus_tag="IU325_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAP5810570.1"
                     /translation="MTFMKGLPLLLLVASLCSYAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTAKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQA
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGSYSGRQFYLGYMD
                     DYGALRMTTLNCSGQCRLQAVEAKK"
     gene            4040..5101
                     /locus_tag="IU325_16345"
     CDS             4040..5101
                     /locus_tag="IU325_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP5810571.1"
                     /translation="MSAGKGVLLVICLVFLPLKSALALNCYFGSSGGSVEKSEVIQPF
                     AVPGNAKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDASLGKSRIDTNQCIDSKNINIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLIYEKTYTAELSSIPG
                     TPIEAGPFDTVVLFKINYN"
     gene            5253..6299
                     /locus_tag="IU325_16350"
     CDS             5253..6299
                     /locus_tag="IU325_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP5810572.1"
                     /translation="MPILQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLTILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRTA"
     gene            complement(6296..7087)
                     /locus_tag="IU325_16355"
     CDS             complement(6296..7087)
                     /locus_tag="IU325_16355"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HAP5810573.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKSYQSRLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVETGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLSAARLDALAHALLDIPRLSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7123..7857)
                     /gene="pxpA"
                     /locus_tag="IU325_16360"
     CDS             complement(7123..7857)
                     /gene="pxpA"
                     /locus_tag="IU325_16360"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HAP5810574.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRRAMQLPPETVYAQTLYQIGALAAITRAQGGVMCH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALEQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKGIVVAA"
     gene            complement(7847..8779)
                     /gene="pxpC"
                     /locus_tag="IU325_16365"
     CDS             complement(7847..8779)
                     /gene="pxpC"
                     /locus_tag="IU325_16365"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HAP5810575.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8773..9429)
                     /gene="pxpB"
                     /locus_tag="IU325_16370"
     CDS             complement(8773..9429)
                     /gene="pxpB"
                     /locus_tag="IU325_16370"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HAP5810576.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9452..10195)
                     /locus_tag="IU325_16375"
     CDS             complement(9452..10195)
                     /locus_tag="IU325_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 GTP cyclohydrolase I"
                     /protein_id="HAP5810577.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGISVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10466..11947
                     /gene="dtpD"
                     /locus_tag="IU325_16380"
     CDS             10466..11947
                     /gene="dtpD"
                     /locus_tag="IU325_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HAP5810578.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDTSGAINY
                     SINAYIEVFDQITWGALACVGVVLMIWLYQALKFRNRALALES"
     gene            complement(11990..13408)
                     /gene="phrB"
                     /locus_tag="IU325_16385"
     CDS             complement(11990..13408)
                     /gene="phrB"
                     /locus_tag="IU325_16385"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HAP5810579.1"
                     /translation="MTTHLVWFRQDLRQHDNLALAAACRNSSARVLALYIATPRQWAA
                     HNMSPRQAEFINAQLNGLRIALAEKGIPLLFREVDDFAASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVQVERTLRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGVPECVAAPKVRSSGSIDPALSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLNEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPTYLKAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEEFDREGEFIRQWLPELRDVPGKVVHEPWKWAQKAGVTLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13405..13914)
                     /locus_tag="IU325_16390"
     CDS             complement(13405..13914)
                     /locus_tag="IU325_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1722 domain-containing protein"
                     /protein_id="HAP5810580.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALNDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVIVLLSHPANRRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMAFYPDAWLSEQRYFELWPRV
                     INLRHAGVL"
     gene            complement(14056..14748)
                     /locus_tag="IU325_16395"
                     /pseudo
     CDS             complement(14056..14748)
                     /locus_tag="IU325_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759736.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3592 a         3916 c         3539 g         3716 t
ORIGIN      
        1 tatactttcc tgccggtagc attgttaacc caatgaaaat tatgcatatc catcccaccc
       61 catctttcat ctcattaaac aataaattta tgcactcaat tattccattc tttctataac
      121 aagcttttta atattgttaa ataaaaccaa atacattcaa ctgtcagtta atatattctt
      181 gcctcgcaac actctatctc attatttaaa ggactcagct atgtttaaag gacaaaaaac
      241 attggccgca ctggccgtat ctctgctgtt cactgcacct gtttatgcgg ctgatgaagg
      301 atccggtgaa attcacttta aaggtgaagt tattgaagca ccgtgtgaaa tacatcagga
      361 tgatattgat aaagaggttg aactcggtca ggtgaccacc agccacatta atcagtcgca
      421 tcacagcgat gccgttgctg tcgacctgcg cttagtcaac tgcgatctgg aaaactccaa
      481 caacggttcc ggcggcaaga tttctaaagt tgcagtcacc tttgacagct cagcgaaaac
      541 caccggcgca gatccgattc tcaacaacac cagcacgggt gaagcgaccg gtgtcggcgt
      601 gcgtttaatg aataaagatc aaagcaatat cgttttaggc accgctactc cagatatcga
      661 cctggcacca acctccagcg aacaaacgtt aaatttcttt gcgtggatgg aacaaattga
      721 tcaggctacg cccgtaacac caggtgcagt tacggcgaat gcaacctacg tgctggatta
      781 taaataagta tcactttggc ggcgctatcg ccgcctttct tcaaatattt atcaggtatg
      841 gacaccgtga atatttatcg actctctttt atatcctgcc tagtcatggc gataccttcc
      901 gcattggcgg tcgaattcaa cctgaatgtc ctcgataaat cgatgcgaga tcgcattgat
      961 atttcattat taaaggaaaa agggggcatt gctcccggtg aatattttgt tagcgttgca
     1021 gtaaataaca accaaatcag taacggacaa aaaattgact ggaaaaagaa cggtgaccaa
     1081 accattccgt gtattaatga tttactggta gataaatttg gtttaaaacc tgaagttcgt
     1141 cagtcgttac cacggttaaa ccagtgcgtc gattttagct cccggcctga aattctcttc
     1201 atttttgatc aggctagcca gcaactgaat atcactattc cacaagcctg gctggcgtgg
     1261 cactcagata actggactcc cccctcaaca tggaaagaag gtgtcgccgg cgtcctgatg
     1321 gactacaacc tgtttgccag cagctaccgc ccacaggatg gtagcaacag cactaacctg
     1381 aacgcctatg gaacagccgg aattaacgcg ggagcatggc gtttacgcag tgattaccaa
     1441 ttgaatcaga ccgatagcga tgataaccat gaacagtcag gcgaaatatc acgcacctat
     1501 ctttttcgtc cattaccgca attaggttca aaattaaccc ttggtgaaac ggatttcagt
     1561 tccaatattt tcgacggctt ttcttatacc ggcgcggcac tggcaagtga cgatcggatg
     1621 ttaccgtggg agctacgcgg ctacgcccca caaatcagcg gcattgcaca aaccaacgcc
     1681 accgtcaccg tgagtcactc agggcgagtt atttaccaga aaaaagtcct accaggtccg
     1741 tttatcattg atgaccttaa tcagtccgtc cagggtacgt tggatgtcaa agtgacggaa
     1801 gaagatggtc gggtgaacaa tttccaggtt tcggcagcat cgacgccttt ccttacgcgc
     1861 caggggcagg ttcgctataa acttgctgcc ggtcagccgc gaacatctat gtctcatcaa
     1921 actgaaaatg aaaccttttt tagcaatgaa gtttcctggg ggatgctgtc aaatacctca
     1981 ctgtacggcg gcctgctgat ttcgggtgac aactaccatt ctgccgcaat gggtatcggg
     2041 caaaatatgc tctggcttgg tgcattgtcg tttgatgtca cctgggccag tagtcagttt
     2101 gatactcagc aggacgagcg gggcttaagt taccgcttta attacagcaa acaagtggat
     2161 gccactaaca gcacgatttc gctcgccgct tatcgcttct cagatcgtca ttttcacagc
     2221 tacgccaact atctggatca caaatacaac gacaacgatg cgcaggacga aaaacagacg
     2281 atcagcttat ccgtggggca accgattacc ccactgaacc tcaatctgta cgccaacctg
     2341 ctgcatcaaa cctggtggaa tgcagacgcc tccacgaccg ccaacatcac ggcgggtttt
     2401 aatgttgata ttggtaactg gagagatatc tcgatctcga cgtcattcaa tacaacccat
     2461 tacgaagata aagatcgcga caatcagatt tacctgtcga tttcgctccc cttcggtaac
     2521 ggggggcggg tcggttatga catgcaaaac agtaatcaca gcaccacgca ccgcatgtcg
     2581 tggaatgata cgttagatga acgtaatagc tggggcatgt ctaccggact gcaatccgac
     2641 cgtccggata atggagccca ggtgagcggt aactatcagc acctgagttc agcgggtgag
     2701 tgggatattt ctggtaccta tgccgccaat gattacagtt ccgtcagcag cagctggagc
     2761 ggttctttca cggcaactca atatggtgcg gcgttccatc gccgcagctc caccaatgaa
     2821 ccacgcctga tggtcagcac cgatggcgtg gcagatattc cggttcaggg caatctcgac
     2881 tacaccaacc attttggcat tgcggtggtg ccgttgattt ccagttacca gccttccacc
     2941 gtggcggtga acatgaatga tttacccgac ggcgtaacag tggcagataa cgttatcaaa
     3001 gaaacgtgga ttgaaggcgc gataggttac aaatcgctgg cttcccgttc cggtaaagac
     3061 gttaacgtca tcattcgcaa cgccagcggt cagctccccc ccctcggtgc ggatatccgc
     3121 caggaggata gcggcattag cgtgggaata gttggtgagg aaggacacgc ctggttaagt
     3181 ggagtcgctg aaaatcaaca atttaccgtg atctggggtg atagccagcg ttgctcgatc
     3241 catcttcctg aacatatgga agacaccgca aatcgcctga ttttaccttg tcattaatta
     3301 aggaaatgaa aatgacattt atgaaaggac tacccctttt gttgttggtt gccagtctgt
     3361 gcagctacgc tgcgctacaa cccgatcgca ctcgcattgt gtttaacgct aatgataaag
     3421 ccaccagcct gcgagtggat aatcgtagcg ataaactccc ctacctcgct tattcctggc
     3481 tcgaaaatga aaaaggggag aaaagcgatg atcttctggt cgccctgcca ccgatacaac
     3541 gtctggaacc gaaagcaaca acgcaggtgc ggatagtcaa gcaagcctcg accgcgaaat
     3601 tacctggcga tcgcgaaacg ctatttttct acaatatgcg tgaaattcct cccgccccag
     3661 aaaaaaatag cgaccatgcc gttctgcaag tcgccattca gagtcgtatc aaggtattct
     3721 ggcgaccggc tgcattacgc aagaaagcag gagaaaaggt cgaactgcag ttacaggtca
     3781 gccagcaggc caaccaactg acactgaaaa atcctaccgc gtattatctg accattgcct
     3841 atctggggag aaatgaaaaa ggcgttctcc ccggcttcaa aaccgtgatg gttgcgcctt
     3901 ttagtacggt caacacaaac accggaagtt atagcggcag gcagttttat ctcggttata
     3961 tggatgatta cggcgcgtta cgcatgacga cgctcaactg cagcggacaa tgccgtttac
     4021 aagcagtgga ggcgaagaaa tgagtgctgg caagggagtg ttgctcgtca tctgtctggt
     4081 atttctgccg ttgaagtctg cgcttgccct gaactgctac tttggttcat ccggtggttc
     4141 ggtagaaaaa tcagaagtga ttcaaccttt tgctgtgcca ggtaatgcca aacccggcga
     4201 taagatctgg gaatctgacg atattaaaat tcccgtctat tgtgacaaca ataccaacgg
     4261 caactttgaa agcgaacatg tctacgcatg ggtaaacccc tacccggggg tacaggatcg
     4321 ttattatcaa ctcggcgtga catacaatgg tgtcgattac gatgccagtc tggggaaaag
     4381 ccgcatcgac accaaccagt gtatcgacag taagaatatt aacatttata cccctgagca
     4441 gattatcgcg atgggctggc agaacaaaat atgctcgggt gaccccgcta atattcacat
     4501 gtcgcgaacc tttctcgctc gtatgcggtt atacgttaag attcgcgaaa tgccgccgca
     4561 tgattatcaa agtacgctta gcgactacat cgtcgtgcaa tttgatggtg ccggtagcgt
     4621 taacgaagac cctaccgccc aaaacctgaa ataccatatt actggtctgg aaaacattcg
     4681 tgtactggat tgcagcgtca atttttctat ttcaccagaa acacaggtga ttgattttgg
     4741 taaatttaac ctgctggata ttcgccgcca cacaatgtcg aaaacattca gtattaagac
     4801 gacaaaatca caaaatgacc agtgcaccga cggatttaag gtcagctcct ccttttatac
     4861 cgaagaaacg ttggttgaag aagataaagc attgttgatt ggtaatggtc tgaagctacg
     4921 tttgctggat gaaaacgcct cgccttatac cttcaataaa tatgctgaat acgccgattt
     4981 caccagcgat atgctgatct acgaaaaaac ctatacggct gaactttcgt ccattccagg
     5041 tacccccatc gaggctggcc ccttcgatac agtggtgctt tttaagatta actataactg
     5101 atttgccatg atgccctttg cagccggaaa gggcatctct ttcagaaaat tgctgatttt
     5161 ctccttttcc ttgattgctg ttattttttc ctggcccggc tttctttatg ctgtatcgat
     5221 acaccaaaga aaaaataatg agggaacgaa ggatgccaat tttgcagtgg ggaatgttat
     5281 gtgtgctctc acttctcctt tctattggtt tcctcgcggt acatctcccg gcggcgctat
     5341 tacttggacc gatgatcgcc gggatcatct ttagtatgcg cggaattact ttacaactcc
     5401 cccgctccgc ttttctcgcc gcgcaggcca ttcttggctg tatgattgcg caaaacctca
     5461 ctggttcgat tctcaccacg ctggccgtca actggccgat cgtgcttaca attttactgg
     5521 tgacgctgct ttccagcgcc attgttgggt ggctactggt gcgctatagc tcattaccag
     5581 gaaatactgg tgcctggggt tcttctcctg gcggcgcggc ggcaatggtc gctatggcgc
     5641 aagattacgg cgcagatatt cgcctggtgg cgtttatgca atatctgcga gtgctgttcg
     5701 tcgcaggggc cgctgttctg gtcacccgca tgatgctggg cgataacgct gaagcggtta
     5761 accagcagat cgtctggttt ccgccagtga gcattaatct cctgcttacg attttgctcg
     5821 cggtcgttgc tggtacggcg ggatgcttgc tacgtcttcc ttccggcacg atgctcatcc
     5881 cgatgctggc gggcgcagtg ctccagtctg gtcagctcat caccatcgaa ctaccagaat
     5941 ggctgctggc gatggcgtat atggcaattg gttggcggat tggtcttggt ttcgataaac
     6001 aaatattact gcgggcattg cgcccactgc cacaaatcct gctgtcgatt tttgctctgc
     6061 tggctatttg tgcaggtatg gcgtggggac tgacccggtt tatgcatatt gattttatga
     6121 ctgcctacct cgccaccagc cccggcgggc ttgataccgt ggcggtcatc gccgcaggga
     6181 gcaatgccga tatggcgctt attatggcga tgcaaaccct gcgcctgttc agtattttgc
     6241 tgacgggacc tgccattgcg cggtttattt caacctatgc gccgaaacgt acggcctagt
     6301 gctgacagcc tgggcaccag taaaacgggc gagatgacag cgtggttttc tcaatgatgc
     6361 tgccacagcg ttcacacggt tcgccatcac gatgaaaaac cttaaagcga aacaacgccc
     6421 catgatgttt attctcatcc acctgccccc gcgtagcgta agaaagtcga ggaatatcca
     6481 gtaacgcgtg tgcgagtgca tctagtcgcg ccgcactgag atctttcgct ttatgattgc
     6541 cggttaaccc aacctgccag aggatctcca cccgcaaata attgccaagc ccagccagaa
     6601 acgcctgatc gagtagtaat ccagcaaact gacggttacg aaagcgcggc gacaataacc
     6661 gttcctttac cacctccggc gtcagattcg gatccagcac atcgggacca acgcgttgta
     6721 aaaacggatg cgtggtcagt tgttccgggg tcaacatctc aatatccgag gcgctataaa
     6781 gcagaatggt tttgtcagcc gtttgcagtt ttacccgcaa tactcgcgtg gtctgcggct
     6841 cttcgccggt ttcaaccacg cgccagacgc cgtaaagctg attatggctg tagagcgtta
     6901 agtcgttgga aaaatgagtt aacaacgcct taccacgcgt ttccacatgg gtaacgtgtt
     6961 gaccgataag tcgtgattga taagatttta actgcgggaa ggcaaaccag acatcagtta
     7021 gtggtttgcc tttgatcgcc gcctccaggt tatccgctgc acggcggatc tccgggcctt
     7081 caggcatggt gatatccttt tttcattctt tattaagcgg ggttatgctg cgacaacaat
     7141 ccccttttcg gcaaatgtag agcgtagtcg gcgggcgaac gccagtgcgt gctcgccatc
     7201 gccatgcagg cagaccgttt gcgccgtgac cgttgcccat tcgccggtga tgcttttgac
     7261 tctgccgtgt tgcaccatct ccaacgtttg ctccagcgcc tgttcttcgt tttcaatcaa
     7321 cgcgcctggc tggcttcgcg gcaccagcga accgtctgcc tgataaccgc gatcggcaaa
     7381 cacttcctcg cgcgttgtca ggccatattg cttgcctgca cgaatcagct cgcttcctgc
     7441 cagcccgacg agaatcaatg ccggatcgca agcgtatacc gctctggcga tggcgtctgc
     7501 cagttgtgcc tctttcgccg cctggttgta taacatgccg tgcggtttga catgacacat
     7561 tacgccgcct tgcgcacggg taatcgctgc cagcgcgcca atttgataca gcgtctgggc
     7621 gtaaacggtt tctggcggca gctgcatggc tctgcgacca aaattttccc tgtcgggaaa
     7681 actcggatga gcgccaatcg caacaccatt ttttattgct tcacgcacgc aagcctgcat
     7741 ggtttgcgca tcgcctgcat gaaatccaca agcaatattg gcagaggaaa ccagcgttaa
     7801 tagctctgcg tcgctggcgc agccttcgcc cagatcggcg ttcaggtcaa ttttcattgt
     7861 gcagccgcca cgctaattgt tcgaaataac gttgttgatc ttgccgcgct ttcagtgcct
     7921 cttccagtga acactggaca aaatgaatcg gctgaccgag cggaatttgc gccagatggt
     7981 acatatcagc ctcaatgata caggcaatac gcggataacc accggtggtc tgtgcgtcgt
     8041 tcatcaatac aatcggctgg ccgttatgcg gcacttgcac cacacccggc aacaaaccgt
     8101 gagataacag ttcgcgatcg gtggtgcgtt ttaaaatttg cccctgtaag cgatagccca
     8161 ttcggttact ttgcgagcta agctgccagg gcgaacgcca gaatgcatcc tgcgaggcgc
     8221 gatcgaactc atgatattcc ggccccggca aggcgcgaat gcggttgccc cacagcagct
     8281 gtttaacgcc ctgcgcttcc ataaaatcac gcttcgcttt gccaatcggg agtcggtcac
     8341 catccttcag taaacggcct tccagcccgc caatccccac tttgagatcg gtgctgcatg
     8401 accccattac cggcggaaca tcaataccac ccgcgaccgc cagataactg cgcatcccgt
     8461 gctgcgggcg tttaagcgtt aaacgctggc ctgctttcat cggcaatcgc cagccagtcc
     8521 agacggcatt atcatccagc cgcgcttcgc aacctgcccc cgtcagggca aaccacccat
     8581 cagtttcgaa ctctaccgtt aactgaccga gcgtgatctc cagcgcgggg gcattggcgt
     8641 cattacccac cagcaggtta gcaatgcgta acgcaggcat atccagtgcg ccgcagtggc
     8701 tgatacccga ctggcgaaaa ccgtgacgac cgccatcctg cacagtggta tacatgcccg
     8761 cacgaataat cttcagcata ctccctcctt ctgcggtaca aagcgcacgc tgtctcccgg
     8821 acgtaataag atgggttcgt cacgcgccgg atcaaacagg ctgagtgagg tatgaccaat
     8881 caactgccag ccacccggcg ttgccagcgg ataaacacca gtctgcggcc cgccgatccc
     8941 gacagaacct gccggaacga gcaagcgcgg ttcagcgcga cgtggcgtgt gtagttgttc
     9001 cggcaaactc ccgagatacg ggaagcccgg ttgaaaacct aaaaaccaga ccacgtattc
     9061 cacggaggag tgcaattcaa caacctgttt ttcgctcaac ccacaatgcg ccgcgaccac
     9121 cgccaaatcc ggtccgcctg cgccaccgta aacaaccgga atttcaataa aacgagactc
     9181 cggctccagc gcctcactct cctcccacca gcgttgcaaa cgctctatgg catccagcgc
     9241 cagcgactca ggattacgta aaatcaccgt gatattgttc atgccgggaa tggcttcaac
     9301 cacattcggc atatccacca gacgctgtgc cagtcgccag atccgtttct ggctagccag
     9361 cgtcaccggc ggttccagtt ccagcactac cgccgtttca cctatcagat aacaacgcgc
     9421 tcgttgcact ttcgatccct ctgattattc gttatgcagg attaggaata tcaataaagg
     9481 tcacatcaag atcggtattt tcattcagcc actcgctcaa cgcgcgaata ccaccacgtt
     9541 cagtggcatg gtgacccgca gcataaaaat gcaatccctg ctcgcgggct gaatgaatgg
     9601 tctgttcaga aacttcgcca gtaataaaag catccacgcc aaaacgcgcg gcgctatcga
     9661 taaaactttg cccgccgccc gtgcaccagg caacgcgctg gaccacctca ggtccggtat
     9721 cgccacacca taacggctta cgtcccagac gcgcttcaat ccaggaggct agttccagtc
     9781 ccggcacagg catggtcagt tcgccccacg gcaccaacgg ctcaatttcg cccatgaccg
     9841 agatccccag taacgccgcc agctgtgcgt tattgcccag ctcaggatgt gcgtcaagcg
     9901 gcaaatgcca gccatacagg ttgatatcat ttgccagcaa cgtttttaaa cggttacgct
     9961 tcatgccgcg aatgaccgga gattcgcctt tccagaagta gccatgatgc acaatgaccg
    10021 catccgcgcc cagacgcact gcctcatcga gcaaagcctg gctggcagtg acaccggtaa
    10081 caattttttg caccgtctct ttgccttcca cctgcaaacc attcggcgcg taatcactaa
    10141 tcgccgcgct gttcagtttt tcgttaatca gttgttccag ttcggtgttt ttcatcttcg
    10201 cgccttccgt attctgatag ctacaccgtt aacatagcgt tacctcgcct gttcgtcgat
    10261 agccaatctt ttgacatatt aagaaacaaa cccgacgcca aaagacgcaa attgtttata
    10321 tcatcacgct tcagctaatg tatatttatg caatcagaca gcatattgca taaataatca
    10381 gatctgtatc aaagttttaa ctttaaggaa acacaagcac gccgcgcaac tattacgtga
    10441 cgttgcagtc aattgtggaa ttattatgaa taaacacgca tcacagccgc gcgctattta
    10501 ctatgtcgtt gcgctgcaaa tctgggaata ttttagcttt tacggcatgc gtgccctgct
    10561 gattctctat ctcaccaatc aactaaaata caacgatact cacgcctacg agttatttag
    10621 cgcctactgt tcgctggtgt atgtcacgcc aatcctcggt ggctttttgg cggataaagt
    10681 tctcggtaat cgcatggcgg tgatgctggg ggcgttgttg atggcgatcg gtcatgtggt
    10741 gctgggtgcc agtgagatcc atccgtcatt cctctatctg tccctggcga ttatcgtttg
    10801 tggctatggt ctgtttaaat ccaacgtcag ttgcctgctc ggcgagctgt atgagccaac
    10861 cgatccgcgt cgtgatggcg gtttctcgct gatgtatgcg gcgggtaacg tggggtctat
    10921 tatcgcacct atcgcctgtg gttacgccca ggaagagtat agctgggcga tgggctttgg
    10981 cctggcggcg gttggcatga tcgcgggtct ggtcattttc ttatgtggca atcgtcattt
    11041 cactcatacc cgcggcgtta acaaaaaagt actgcgtgcg acaaactttc tcctgcccaa
    11101 ctggggatgg ctgctggttc tgctggtggc aacgcctgcg ctgatcaccg tactgttctg
    11161 gaaagagtgg tcggtatacg ccttgattgt cgcgactatt attggcctgg gtgtattggc
    11221 aaaaatttat cgcaaagcag aaaaccagaa acagcgtaag gagctggggc tgattgttac
    11281 cctcaccttc ttcagcatgt tgttctgggc attcgcacaa caggggggta gctcgattag
    11341 cctttatatc gaccgcttcg ttaaccgcga tatgtttggt tataccgttc cgactgctat
    11401 gttccagtcg attaatgcct tcgcagttat gctgtgcggt gtgttcctgg cgtgggtggt
    11461 taaagagagc gtcgcaggta atcgtaccgt gcgcatctgg ggaaaatttg ctcttggcct
    11521 tggcctgatg agcgccggat tctgcattct gaccttaagc gcccgctggt ccgcaatgta
    11581 tggtcactct tctctgccac taatggtgtt aggcctggcg gtgatgggct ttgcggaact
    11641 gtttatcgac ccggttgcta tgtcgcagat tacgcgcatt gaaatccctg gcgtgactgg
    11701 cgtattaact ggcatttata tgctgctttc cggcgcgatt gcgaactatc tggctggcgt
    11761 gattgccgat cagacatcgc aggcttcgtt tgatacttcc ggggcgatca actactccat
    11821 caatgcatat attgaagtat ttgatcaaat tacctggggc gcactggcgt gtgtaggagt
    11881 ggtgttgatg atttggctgt atcaggcgct gaaattcaga aaccgcgcgc tggcgctgga
    11941 gtcttaacag gccagcaccg atgcctgata aaacgctcgc tgcgttttat caggctcctt
    12001 tcctcgcttc ttcgtacgct gccagcgttc gcagccttgc ctctttgtga tcaactatcg
    12061 gttgcggata atccagcgtc acacctgctt tctgcgccca cttccacggc tcatgcacca
    12121 ctttccctgg cacatcgcgc agttccggta gccactggcg gataaactcg ccttcgcggt
    12181 caaattcctc gccctgggtt gtcgggttaa aaatacgaaa atacggcgct gcatcggttc
    12241 cggttgaagc ggcccactgc cagccaccgt tattggctgc caaatcacca tcaatcagct
    12301 gcgacatgaa atatcgctca ccttcgcgcc agtcgatcaa taaatctttc accaaaaaac
    12361 tggctgtaat catccgtagc cggttatgca tccagccagt gctgttaagc tgacgcatgg
    12421 cggcatcgac aatcgggtat ccggttttac cttcctgcca ggcctttaaa taagtgggat
    12481 tgctttgcca ctgtacacga tccgtccagg caataaatgg acgatgttta cacaacgaag
    12541 ggtaatacgt catcagatga cggtagaact cgcgccagat cagctcatta agccagacac
    12601 taccggcccc accgtccagc gcctgcggct gttccgccaa caggcgatgc aaacactggc
    12661 gaggcgataa cccgcccgtt gccagactgg cggacaaacg actggtgcct tccactgccg
    12721 gaaaatctcg ttgttgctca tactctccgg caccgttctg gcaaaactgg cgtaattgcg
    12781 caatcgccgc tttttcttcc accgggaaat gcgcagtatc gaaagactga cgagggtaat
    12841 tcagcgtaat tgatagcgcg ggatctatcg atccactact acgtactttt ggcgcagcga
    12901 cgcactccgg caccccttct cgcaaccgtt tcagccaggc gtttttaaat ggcgtaaaga
    12961 ctttgtacat ctcatgattg ccagtcatca ccgcccccgg cggcaggatc acgctgtcgt
    13021 caaatccttc acacaccacg ttacgcagcg ttctctcaac ctgcacatcc cgcgcccgct
    13081 cattcacttc atactgatag ttataaaaaa gatgagtaac gctgttttcc gcgcacacct
    13141 gtttaactat ttcgacactg gcggcaaagt catccacttc gcggaacaat aaaggaatgc
    13201 ctttttccgc cagcgctatt cgtagcccgt tcagttgtgc attgataaat tcagcctgac
    13261 gcggcgacat attatgcgcc gcccactggc gtggtgtagc gatatacaac gccagcacgc
    13321 gtgcagacga attgcggcag gcggcagcca gcgcgagatt atcgtgctga cgtaaatcct
    13381 ggcgaaacca gaccagatgg gtagtcataa aactcctgca tggcgcaagt taatcacacg
    13441 cggccaaagt tcgaaataac gctgctctga aagccaggcg tcaggataaa acgccatata
    13501 gtgtttgata cgcatcagcg gcgcaagaag cggttgctcg ccacggcgat aactgtcgat
    13561 aagcgccgcc agctgctggc gttctgtgga atcaatatgt gggcgaaaat atccctgaac
    13621 gtgcatcaaa acgttagtat gatcacgccg gttggcaggg tgagaaagca aaacaattac
    13681 gcgttggcgg tactggttgt aatagtcatc cagattttgc cactggtgaa tatcagccac
    13741 aaacgggcca agtttgcggt actccggctg ggaatgggcc agaaagacga gtttatagcg
    13801 gctgtgataa tcgttgagtg ccccgcgcgt taagccatgt tctttcagtc gatgtaactc
    13861 atgaagggcg aagatacgga ttagggtgct gtcatcaaat cgttgctgat tcattactgc
    13921 tcctgaacgg tttacaggag taagtgtaga cgttgggaaa tgatatttgt gaagttaatc
    13981 ctgttgtagc agggcaagat tacgaaagcc cgctccccgc aaggactgac gtcaggtagt
    14041 ttctgtccat ggctgccctt tccattcaaa cgtgaaatca ataagttcat cagggacatc
    14101 gcaaacaata tgaagacgga tttcttctct gccgtgactc ttttcactca ttgcgtaact
    14161 gtcatgctct ggattattta attctttcag tgggaatttt tcctcaaagg ctttatttag
    14221 tcgtccctga tttcctttta cagcgaatac ataatcaccg ccctgttttt gtatcttctc
    14281 tgcaatatct ttctggcaac ccatcgcatc agttgtgatg atttttcctt taatatccaa
    14341 catgttaaga agttcaggga tagctgtgat ctcattggat ttctcatccg tcctgatctg
    14401 tccgatgacc agactgtgca ttgttgagaa cgcactaatg acatgaatcg ctcccctgcg
    14461 gcgactcttg tcataagaat gccggagcgt ttttccatca attgcaataa cgtctttatc
    14521 atctgaagaa tggcagtcac gcatccagtt aataaagcac tcgtgaaatt ttgaaggact
    14581 gatacaggat acaactctgg cgatggtatc gtgaacagga ataccatttt aaaaatcacc
    14641 atattgtttt aaaaaatcga gatgtgtttc cccaaaatcc tctatatctt cccaaccgtc
    14701 tgcacaagaa ataacggcac aaatagtcaa cagtagaata tccgacaatt tatgttccac
    14761 ttt
//
LOCUS       DACBVI010000111        14638 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.111, whole genome shotgun sequence.
ACCESSION   DACBVI010000111 DACBVI010000000
VERSION     DACBVI010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.111"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            388..903
                     /locus_tag="IU325_16400"
     CDS             388..903
                     /locus_tag="IU325_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP5810581.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLEQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            1121..1849
                     /gene="artP"
                     /locus_tag="IU325_16405"
     CDS             1121..1849
                     /gene="artP"
                     /locus_tag="IU325_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HAP5810582.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            1867..2598
                     /locus_tag="IU325_16410"
     CDS             1867..2598
                     /locus_tag="IU325_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP5810583.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGRYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            2605..3321
                     /gene="artQ"
                     /locus_tag="IU325_16415"
     CDS             2605..3321
                     /gene="artQ"
                     /locus_tag="IU325_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HAP5810584.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPMGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPG"
     gene            3321..3989
                     /gene="artM"
                     /locus_tag="IU325_16420"
     CDS             3321..3989
                     /gene="artM"
                     /locus_tag="IU325_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HAP5810585.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLAVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            4215..4946
                     /locus_tag="IU325_16425"
     CDS             4215..4946
                     /locus_tag="IU325_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP5810586.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQEQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(4975..6102)
                     /gene="rlmC"
                     /locus_tag="IU325_16430"
     CDS             complement(4975..6102)
                     /gene="rlmC"
                     /locus_tag="IU325_16430"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HAP5810587.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLAKQ"
     gene            complement(6143..6631)
                     /locus_tag="IU325_16435"
     CDS             complement(6143..6631)
                     /locus_tag="IU325_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2593 family protein"
                     /protein_id="HAP5810588.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILLLLFVPSTSRRFFQ
                     LQ"
     gene            complement(6691..7536)
                     /gene="potI"
                     /locus_tag="IU325_16440"
     CDS             complement(6691..7536)
                     /gene="potI"
                     /locus_tag="IU325_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HAP5810589.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(7533..8477)
                     /gene="potH"
                     /locus_tag="IU325_16445"
     CDS             complement(7533..8477)
                     /gene="potH"
                     /locus_tag="IU325_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HAP5810590.1"
                     /translation="MSTLEPAAQPKPFKLWLSQLQMKHGRKLVIALPYIWLILLFLLP
                     FLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQSLQV
                     AAISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKNNGV
                     LNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAALDL
                     GARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQEFF
                     NNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(8487..9620)
                     /gene="potG"
                     /locus_tag="IU325_16450"
     CDS             complement(8487..9620)
                     /gene="potG"
                     /locus_tag="IU325_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HAP5810591.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFECVLKERQEDGLVLYSPGLV
                     HPLKVDVDASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(9715..10827)
                     /gene="potF"
                     /locus_tag="IU325_16455"
     CDS             complement(9715..10827)
                     /gene="potF"
                     /locus_tag="IU325_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HAP5810592.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(11178..11654)
                     /locus_tag="IU325_16460"
     CDS             complement(11178..11654)
                     /locus_tag="IU325_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HAP5810593.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(11742..12644)
                     /gene="rimK"
                     /locus_tag="IU325_16465"
     CDS             complement(11742..12644)
                     /gene="rimK"
                     /locus_tag="IU325_16465"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HAP5810594.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(12705..13427)
                     /gene="nfsA"
                     /locus_tag="IU325_16470"
     CDS             complement(12705..13427)
                     /gene="nfsA"
                     /locus_tag="IU325_16470"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HAP5810595.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALRDELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENNYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(13411..13698)
                     /locus_tag="IU325_16475"
     CDS             complement(13411..13698)
                     /locus_tag="IU325_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HAP5810596.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSGSLSLPEPFSRPEV
                     QILMIFLGVLLMLPTAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            13858..14115
                     /locus_tag="IU325_16480"
     CDS             13858..14115
                     /locus_tag="IU325_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxA family glutaredoxin"
                     /protein_id="HAP5810597.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(14145..14522)
                     /locus_tag="IU325_16485"
     CDS             complement(14145..14522)
                     /locus_tag="IU325_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810598.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
BASE COUNT         3738 a         3885 c         3591 g         3424 t
ORIGIN      
        1 tctcttatta catgtgaaaa attctgtaat gattagacaa taaccgcgtt catttatcac
       61 gcgttataaa tgtgaaactg gcttcttgca gatgctctta acctctacag gaggatttat
      121 cccgccattt cagaactatc atatttacct taaatgatca tttcagccat ataacacgct
      181 caacctcaca aatggtttac actgtctatt gtttgggtaa tttaagtaag tcagctacga
      241 gcaaccttga gtgggtagcc agcaatggac tcaaacctaa tcaggtgttc agattgtttc
      301 agaattgtac taaaaacaat gtattaaata atctgatagt gtttggtcgt agtgtgacac
      361 agacagcaag ctaataagga aatgattatg cgctaccgtt cactatctct acttattcca
      421 tgtgcactgc tgctgagtgc ctgtaccacc gtcacgccag cttataaaga taacggcccc
      481 cgcaccggtt cctgtgtcca gggtggccct gacagcgtgg ctcaacaatt ctatgactac
      541 cgcattcaac atcgcagcaa tgacattacc gccctgcgcc cctatttgag tgacaaactg
      601 gcaaccctac ttagcgatgc cagccgcgat aatagtcatc gtgaattgtt aagcagcgat
      661 ccgttctcca gtcgaaccac cttgccagac agcgcccacg ttgccagtgc atcgactatc
      721 cctaatcgtg atgcccgtaa cattccattg cgcgtggatc tcgaacaggg tgaccaggga
      781 tggcaggatg aagtgttaat gattcaggaa ggccagtgtt gggtcatcga cgatgtgcgc
      841 tacttaggcg gtagcgttca cgccacagcg ggtacgctcc gtcagtccat cgagaatcgc
      901 taaagccttg atcaaccaat aaaccccgta aattgtccgg catttcgtga ggaatgccga
      961 catttatgct cgccgaccac cgcccccgtt attttgtgct atgtttattg aataatgcgc
     1021 tttgctttta acttttaaag cagaaatatt gcataattat tctgtcaaag gtactatctt
     1081 cgacctcaat agctatcaga ctgccagtat acgagtgtca atgagtattc aattaaacgg
     1141 cattaattgc ttctacggcg cgcatcaggc gctgttcgat atcacgctgg attgcccaca
     1201 gggcgaaacg ctggtgttac ttggccccag cggcgcgggt aaaagctcgc tgctgcgtgt
     1261 actcaatctg cttgagatgc cgcgctccgg tacgctcaac attgcaggca accatttcga
     1321 tttcaccaaa acaccctctg acaaagcgat tcgcgatttg cgtcgtaacg ttggcatggt
     1381 gtttcagcaa tacaacctgt ggccgcatct gaccgtgcag caaaacctga ttgaagcgcc
     1441 ctgccgtgta ctggggttga gcaaagatca ggcgctggcc cgtgcagaaa aactgctgga
     1501 acgtctgcgt ctcaaacctt atagcgatcg ttacccgctg catctttctg gtggtcagca
     1561 gcagcgtgtt gctattgccc gtgcgttgat gatggaaccg caggtactgc tgttcgatga
     1621 accgaccgcc gcactggacc cggaaattac ggcacaaatc gtcagcatca ttcgtgagtt
     1681 ggcagaaacg aatattaccc aggtgatcgt cacccacgaa gttgaagtgg cgcgtaaaac
     1741 cgccagccga gtggtgtata tggaaaacgg tcatatcgta gaacaaggcg acgcgagctg
     1801 ctttaccgag ccgcaaaccg aagcatttaa aaactatctc tctcactaat attcgggata
     1861 acaacaatga aaaaagttct gattgccgcg ttaattgcag gttttagtct ttccgccaca
     1921 gctgccgaaa ctattcgttt tgctaccgaa gcctcctatc ctccgtttga atcgattgat
     1981 gcaaacaacc agatcgttgg ttttgacgtc gacctggcac aggcactgtg taaagagatt
     2041 gatgcaacct gtactttctc taaccaggcg tttgacagcc tgatcccaag cctgaaattc
     2101 cgtcgcgtag aagcagtgat ggcgggcatg gatatcactc cggagcgtga aaagcaggtg
     2161 ctgtttacca ccccttacta tgacaactct gccctgtttg tgggtcagca aggcagatac
     2221 accagtgttg atcagctgaa aggcaaaaaa gtcggcgttc agaacgggac gacacaccag
     2281 aagttcatta tggataagca cccggaaatc accaccgttc cgtatgacag ctaccagaac
     2341 gcaaaactgg atctgcaaaa tggtcgtatc gacagcgtat ttggtgacac cgcagtggta
     2401 accgaatggc tgaaagacaa cccgaaactg gcggcagtgg gcgataaagt gaccgataaa
     2461 gattatttcg gtactggcct cggcatcgcg gtacgtcagg gcaacactga actgcagcag
     2521 aaactcaaca ctgcgctgga aaaagtgaag aaagatggca cttacgaaac catctacaac
     2581 aaatggttcc agaagtaatt cctgatgaat gaattttttc ctttagcaag cgccgccggg
     2641 atgaccgtcg gccttgccgt ttgtgcattg attgtcgggc tggcgctggc gatgttcttt
     2701 gcggtatggg agtctgcaaa atggcgtcct gtcgcgtggg caggttcagc gctggtaacc
     2761 attctgcgtg gcctgccaga aattctggtg gtgctgttta tctattttgg ctcctcgcag
     2821 ctgctgctga cgctttcgga tggtttcact atcaatcttg ggttcgtgca gatcccggtg
     2881 cagatggaca ttgagaactt cgacgttagc ccgttccttt gtggtgtcat cgctctgtcg
     2941 ctgctgtatg ccgcctatgc ctcgcaaacg ctgcgcggcg cgttgaaagc ggtgccgatg
     3001 ggtcagtggg aatccggcca ggcgctgggg ctgtcgaaat cggctatctt tttccgtctg
     3061 gtgatgccgc agatgtggcg tcatgcgctg cctggtctcg gtaaccagtg gctggtgctg
     3121 ctgaaagata ccgcgctggt cagtttgatt agtgtgaatg atttaatgct acaaaccaaa
     3181 agcatcgcta cccgtaccca ggaaccattt acctggtaca ttgtggcggc ggcgatttac
     3241 ctggtgatca ccctgctcag tcagtacatt ctcaaacgca ttgacctgcg cgcgacacgt
     3301 tttgagcgga ggcctggcta atgtttgagt atttacccga actgatgaaa gggctgcaca
     3361 ccagcctgac gctaaccgtt gcctcgctga ttgtggcact gattctggca ttgattttta
     3421 ccatcatcct gacgctgaaa acgccggtac tggtgtggct ggtgcggggt tatattacgc
     3481 tgtttaccgg tacgccgctg ctggtgcaga ttttcctgat ttattacggg ccgggccagt
     3541 tcccgacgtt gcaggagtat ccggcactgt ggcatttgct gtcagaaccg tggctgtgtg
     3601 cgctgattgc gttgtcgctg aacagtgcag cgtataccac gcagctgttt tacggtgcca
     3661 ttcgtgcgat cccggaaggt cagtggcagt cctgtagcgc cctgggaatg agcaaaaaag
     3721 atacgctggc gatcctgctg ccgtatgcct ttaaacgctc gctctcttct tattccaacg
     3781 aagtggtgct ggtgttcaaa agtacctctc tggcttacac cattacgctg atggaagtga
     3841 tgggatacag ccagttgttg tacggacgca cctacgatgt catggtgttc ggtgcggcag
     3901 ggattattta cctggccgtc aacggcctgc tgacgctgat gatgcgtctg atcgagcgca
     3961 aagcactggc attcgaacgg cggaattaat gtggctgcgt gctgatgccg gatgcggcgt
     4021 gaacgcctta tccggcctac atagtgcata aattcttata atgaagacgg acaacccact
     4081 aagttgtccg tcttttttta ttccatgtaa attatttaat catatttatt gcatataaat
     4141 tcacttgatg gcattgttat cccatgccgc agacacggcc aaaaatcata agattgacag
     4201 acgggagttc catcatgaaa aagttagttc ttgccgcttt acttgcttcc tttactttcg
     4261 gtgcttctgc cgcagagaaa atcaattttg gcgtttcagc cacctatcca ccctttgaat
     4321 ctataggtgc taataatgag attgtcggct ttgatatcga tctggcaaaa gccttgtgca
     4381 aacaaatgca ggcagaatgt acttttacga atcacgcgtt cgacagcctg atcccgtcac
     4441 tgaaattcag aaaatatgac gccgtaatct ccggtatgga tatcaccccg gagcgtagca
     4501 aacaggtatc gtttaccacg ccctactatg aaaactcagc cgtcgtgatt gccaaaaaag
     4561 atacctacaa aacgtttgcc gatctgaaag gcaaacgtat tgggatggaa aacggcacca
     4621 cgcaccagaa atatattcag gagcagcacc cggaagtgaa aactgtctcc tatgacagtt
     4681 atcagaatgc ctttatcgac ctgaaaaatg gtcgtattga tggggtattt ggtgacacag
     4741 cggtggtaaa cgaatggctg aaaaccaatc cacaactggg cgttgcgact gagaaagtga
     4801 ccgatccgca gtacttcggc accggcctgg gtatcgctgt acgtccggat aacaaagctc
     4861 tgctggagaa actgaataac gcgctggcgg caattaaagc tgacggtaca tatcagaaaa
     4921 tcaatgacca gtggttccca cagtaagatt cagaaagccg cacctgcggc ttttttattg
     4981 cttcgccagc agcgtcagca cttcatagtg cgcggtgtgc gggaacatat caaaaagctg
     5041 tacccgttca atacggtaac ctggcagttc gcggatatct ttcgccatgg tttgggcgtt
     5101 acagctggag tagataataa aacgcggtgc catcgttgag agataatcac acagcggttt
     5161 accaatgcca cgtcgcggcg ggttaaccag caccagctca ggcacttccc cctgggcggt
     5221 ggcaaactga gtggagtcca gcgcctgaaa ttgcaaacgc gttaagccca gttcagcggc
     5281 tgactgcttc gcacaggcaa tggcctctgg tgcaatttcg atcccggtta actgcatgtc
     5341 aggcgtcgcg cagtgtaaac caaagccccc cacgccacag aacagatccc acatatgttt
     5401 aactggcagc tgccgtaccc agtcgcgcgc ggtagcgtac agctggctgg cgaccgccgg
     5461 attagtctgg aagaaacttt gcggacggat ccacagcggt acgtcattaa aacgctctgc
     5521 cagcgcctgt tgttcagtca ggtagatctc cgtctcccct tccataatcg ccatatgtac
     5581 cggctgaata ttgacggtaa taactttcag ctgcggtagt tgttcctgta accacggcag
     5641 cgccttacgc agttgcgcca gcttggtatc agaacgcagt acaaagcgca gcatcatgcc
     5701 gccatcgctc tggctttcag tcagcagaat gtatttcagt tcgccacgct tacgcgccac
     5761 gttgtagggc gttaaccccg ctcgggcgat aaacggtttt agcgccgcaa aaacgggcgc
     5821 aaatgaggca ggataaagcg ggcagtcaca aaggtcttcc ggtgtgccgt ctcgatgcag
     5881 cataccgagc agtggttttt caacgctacc actcaccacc attttggctt tattacgaaa
     5941 cccttgttcc gggcctgaca ctggcgcaca ccattcctca accggaaagt cggcgagcag
     6001 atttttaaga tcggcggttt tagcggagag ttgctctgga atcggctgcg ttatccactg
     6061 acaggaacga cagcgacccg cgtcgtaaag tgcgcactgc atacattgac cttcacatca
     6121 tcaggggcga cgattataca cattattgca actggaagaa ccgccgactg gtcgagggaa
     6181 cgaacagtag caacaggatg agcatatccg gcaacttctg cagcatcagg ctatggaaga
     6241 tttcacgttt tgattcaccg ggaatgctga acagctccgg ataaccatac cccagcgaag
     6301 ctgcccacaa gtaacttgcg gctgtgattt gtgtcaacag atacagccag cgcgcccagc
     6361 gacgcccttt aaccagcgaa aacgcacacc agatctcaat gaaaaccagc accagactgc
     6421 tcaaaaagac cagcgttaaa ctccaggttt gtacgctgcg atgaatgaac tcgccaatac
     6481 cgcgcacgcc cagcgtattg aaaatcatca gcacgtcgag gccacggatc ataataatgg
     6541 cgagcgccgc cacctgcacc agcgcaggca cattcaggcg agcatgagat gaagatgttt
     6601 tcttaaaaaa tcccaacgtt tcgtcttcca tgaaaacaat gccgcgacat gcgcggcatt
     6661 atgtagccag gttggcaaat tttagtgtct tcagccacgt cttgcacgct ggatgtcgcg
     6721 tatccgctgt ttttctgcgc gcgccatcag ataccaggcg ataaatccga caattccgac
     6781 cgcgccgagg atcaatgttg ccagggcgtt gatttccgga ttcaccccca tccgcacgct
     6841 ggaaaagacc agcatcggta acgtggtggc tcccggcccg gaaacaaagc tggcgatcac
     6901 cagatcatca agcgacaaag taaaagccag taaccagcca gaaatgatcg cgggcatgat
     6961 catcggtagc gtaatgacga aaaacacttt caacggcgtc gcaccgagat ccatcgctgc
     7021 ttcttctatc gagcgatcca gttcccgcag acgcgacgaa ataacgactg ccacataagc
     7081 cgtacagaaa gtgacatgcg ccagccagat ggtgagcata ccgcggtccg caggccagcc
     7141 aatggcatga gcaagcgcga cgaataacaa caatagcgac aagcccgtga tgacatctgg
     7201 catcaccagc ggcgcggtga tcataaaggc aaaaccattt gatccgcgaa acctgccaaa
     7261 gcgcaccagc acgaccgccg caatcgtccc gaggatcgcc gccgccgtcg ccgcacaggc
     7321 cgcaattgtc aggcttagac caaccgcact catcattgcg tcatcgcgca ataactcacc
     7381 ataccagcgt gttgaccagc cggcccacac cgtcaccagc ttcgagctgt tgaatgaata
     7441 gatcaccagc atcagcattg gcgcgtagag aaaggtgaag cccagcagca aaatcacaat
     7501 ccgccagggc gaacgaacta ccggtaaatt attcatccgt gttctcccac gcttttttgc
     7561 tgatgcttgt ggaaccacat tatcggcaca attagcagca gcaacatgat gatcgctacc
     7621 gccgaggcca ccggccagtc gcggttgtta aagaactcct gccacagcac gcgcccgatc
     7681 atgatgctgt ccgggccacc gagcagttca gggatcacaa actcgcccac agccgggata
     7741 aacaccagca tcgatccggc aataatcccg cctttggtca gcggcacgat cacagtaaag
     7801 aacgttttca gcggtcgtgc accgagatcc agtgccgctt ccaccagcga ataatcaata
     7861 cgaatcaagg cggtgtagat cggcagaacc ataaacggca cgtaggcata aacaatgccg
     7921 atataaacgg ccagattggt atgcagaatg gtcagcggct ggtcgataac ccccagccac
     7981 agcagaaaat tattcagcac gccgttattt tttaatattc ccatccaggc ataaacgcga
     8041 atcagaaacg aggtccacga cggcaggatc accagtagca ataagatatt acgggtcgaa
     8101 ggcttactgt gcgccaccgc ccacgccagc ggatagccga tcagtaaaca gcaaatagtc
     8161 gaaatcgccg ccacctgtag cgactggaga taagcatcga aatagagcgg atcgtcggtc
     8221 agttgcagga aattaccgag attaagagtg atggaaagtt gcccgtcagc ccactccatc
     8281 agttcggtat aaggtggaat ggcgcgcgcc atctccgcca ggcttatttt aaagacaatc
     8341 agaaatggca gcagaaacag caggattagc cagatatacg gcagcgcaat caccagtttg
     8401 cgcccatgct tcatttgcaa ctgcgacagc cacagcttaa agggttttgg ctgggcagca
     8461 ggttcaagtg tactcatctt tgctccttaa accgtcagca ccacacagct gtccacttcc
     8521 cagcacaaac gcacttcatc gccccaggtc ggtaaccctt tacgatgacg atgggcgttt
     8581 tgtagctggg cgctaatcat ctgcccactt ttcagacgaa cgtgatacac cgaaagatcg
     8641 ccgagatagg caatgtgtat cacctccccc accgcgaagt tacaaccatt ggcgggcggc
     8701 tcttcgcaaa gcatgatttt ttccggacgc agcgctacgt gtaccggcac gttatcgacc
     8761 accgaagcat ctacatcgac tttcagtgga tgcaccagcc ccggcgaata aagcaccaga
     8821 ccatcttcct ggcgctcttt gagtacacat tcaaagacat taaccgagcc aataaattcg
     8881 gcgctgtagc gggtggtcgg atgctcatag atctcttccg gttcgccaat ctggacaaat
     8941 ttcccacgat tcataatggc gatgcgcccc gccatggtca tcgcctcttc ctgatcgtgg
     9001 gtgaccatca cacaagtcac accgacacgc tccagaatat ccaccacttc aagctgcatc
     9061 cggtcacgca gctttttatc cagcgcgccc ataggctcat cgagcagtaa tagtttcggg
     9121 cgtttggcaa ggcttcgggc cagggccaca cgctgtcgct gaccaccgga aagctgatgc
     9181 ggtttgcgtt tggcgaactc ctgcatatgc accaggccga gcatctcatt gacccggctg
     9241 gcaatttccg ctttcggcaa tttgtcctgt ttcaggccaa aagcgatatt ctgttccacg
     9301 gtcatatgcg gaaacagcgc gtaagactga aacatcatat tgatggggcg caggtaaggc
     9361 ggaacctgtg acaaatcgac gccatcgagc ataatctgcc cggcagaagg ttgttcgaaa
     9421 cctgccagca tacgcagcag cgtggacttg ccacagccgg atgcgcccag cagcgcgaag
     9481 atttcgcctt tatagatggt caggctgaca tcatcgaccg catgctgacc atcgtaggat
     9541 ttggtgaggt tgcggatttc taatagcggc gtcagcgcct tacgggtttt cgcctgcggg
     9601 cgagggatag cgtcattcac ggcagctctc cggcattgaa tgtgtgcaag cgcaccataa
     9661 tggtgcgaat tgccggcggg tgtggtgcgc ccgtccggca tctacgactg cggattattt
     9721 tccgctcttc actttggtcc acgcgcgggt gcgcacacgg tcgattttcg gatcctgtac
     9781 tttcagagtg aacagcttcg cacgaacatc cgcaggcggg taaatacccg ggttatcacg
     9841 gacttccgca ctcaccagcg gcgtagctgc tttattggcg ttagcataga acacatggtc
     9901 ggaaatatgc gccaccacat ccgggcgcag caggtaattc aggaactgat aggcttcgtc
     9961 tttatttttg gcatccgcag gcatggcgaa gacatcaaag aacgccatcg ccccttcttt
    10021 cggaatcgag aacgagacat taacgccatt cttcgcttcc ttcgcgcggt ttgacgcctg
    10081 ccagacatcg cccgcccagc caatagcgac gcagatatcg ccgtttgcca ggtcgttaat
    10141 gtattgcgat gaatggaaat aacgaatgtt cgggcgcagc tttaacagca gatcggttgc
    10201 cggtccggtg taatcatctg ctttagtgct gttgggatct ttaccgagat aattcagcac
    10261 ggtagcaaac acttcttccg gtgcatcaag gaaagagacg ccgcagcttt tcagtttttc
    10321 cagattttca ggcttgagga tcaagtccca gctatcgacg ggcgcgtttt cgcccagcac
    10381 cgctttaact ttatcaacgt tatagccaat cccggtcgtc gcccacatat agggcatagc
    10441 aaatttatta tccgggtcgt gtttggcaac caacttcagc agttccgggt cgagattctt
    10501 ccactccggc aatttacttt tatccagcgg ctggaaaact cccgcagtca actggcgttc
    10561 aagaaagctt gctgacggca ccaccaggtc aaagccggtg ctacccgcca ttaatttgcc
    10621 ttccagtact tcgttagagt cgaaaacatc gtagacgact ttaataccgg tttctttttc
    10681 aaaattggcc accgtgtccg gggcgatata atcagaccag ttataaatgt ggagtgtttt
    10741 ttgttcagcc gcgagcgtgc cgacagagac ggccatcaga gcacccgcaa ccagacccga
    10801 tagccatttt ttatttaagg cggtcatatc tctttccttc tgaaagttcg ttaacaaacg
    10861 aacaaaagtt gtcacactga aagatatgca ataaacgtgc atatttttta tcggcgcggt
    10921 gtaagaaaag agaactctct cccggccagc actgctcgct atataaaagc atcgcaagaa
    10981 taactttagc cagggtttga gactatagct cggattaaaa aacggtattt aacaaatttt
    11041 tatgcgtatt tgctctatgt gataaagcca aatcggcaca agagaagtga aataatcttt
    11101 aagaaaaggt taaaaacagc agataatttt gcagcacgca gtcactgcgg cagcgactgc
    11161 gtactcgaaa ggagttatca gtggaggaaa tagttttcgg taacgttgtt ttgcccttca
    11221 tcgtcagtcg gtaacagcag ttgatgggca ttagcttcaa gaatgaccat cgaaatctgc
    11281 tcttcgctct gacgcacgaa ccatgcaaac tgctcatagg ttacgccctg cataacggat
    11341 aaagactggc aaaccaccag ctttggcaga ttatcatcct gcatatcaag aaatgctttc
    11401 acggtcagcg aactggcatt gatggcagat aaatctgccg ccaggggcaa tacggctgaa
    11461 ggtcggactt ccgccatggc agaaaacagg atcgtgttat cgatcagatc gattttggca
    11521 tcaaagacac cgtcgaaatt ctgcatatgg ggcagatgca gagcctgaca gttatcacat
    11581 tcaaaaaaac tcatccccag gtcatcgagc cattgacgca gcgtatccag accaggaacg
    11641 accagcgatg tcataatttt gtacaacctc tttcgataaa aagaccggca cagcttacgc
    11701 aaaaagcgca ggcaaaacca tgatcagtaa tgtgattgcg actaaccacc cgttttcagg
    11761 caatattctg tcgtagcgtg gcgttcgatc cagcggatca ttttacccgc gatgtcgata
    11821 ccggtggttt tttctattcc ttccagcccc ggcgacgcat tcacttccat caccaacggc
    11881 ccgcgattag cacgcagaat atccacacca gcaacgtcca gcgccatcgt tcgcgcggct
    11941 tttatcgcga tttcacgctc ctgtggtgtg atactggcga cgcttgccgc gccgccacga
    12001 tgcaaattgg aacgaaaatc gccctctttc gcccgccgtt caatcgcagc gaccacttca
    12061 tcgccaacaa ccagacagcg gatatcgcac ccttgcgcct ctttgatata ttcctgcacc
    12121 agaatatgcg cgttcagacc gcggaaagcg tcaatcacgc tttcggccgc ctgacgcgtc
    12181 tccgccagca cgacgccaat tccctgcgtg ccttcaacca acttgaccac cagcggcgca
    12241 ccaccgacca tgtcgattaa atcgctggta tcatccggcg aatgcgcaat gcccgtgaca
    12301 ggcaggtcga tgccctgacg cgccagcagt tgcatggaac gcaatttgtc acgcgcccgg
    12361 gcaatggcga ccgactcatt gagcggatag ctccccagca tctcgaactg gcgcagtgcc
    12421 gccgtcccat aaaaggtaat ggcggtgcca atacgcggga tcactgcgtc aaaatggggt
    12481 aacttgcggc ctttgtagtg aatagaagac gccgcaggat ttatgttcat gtagcaagaa
    12541 agcggatcaa gaatttcaac caggtgaccg cgctgtatag cggcttcacg cagccgctta
    12601 cacgaataga gcgttccatc ccgggacaat atggcaattt tcaccctgca cctctctgtc
    12661 agacctggtc aaaagcccgc gtatcataca tcggcgtggc ggttttagcg cgtagcccaa
    12721 ccctgtttgt gcaaataatc cagtataaat gggcggcttt ctttaatgat tgttcggcgg
    12781 atatgatcgc tccaggtatc ccggcgatta ttgctgccac gggtgaggta atattccgcc
    12841 agttgttcgt catactgcgc cagtgcgtct ttatccagcg gttgatagtt gttttcatgc
    12901 accaaaatgg aggccggtaa acgcggctta agatccggat tatccgcagg ccagccaagg
    12961 cacagcccaa acagcggcag aacatgctgc ggtaatttca gcagttccgt caccgcttca
    13021 atattattgc gcaggccgcc gatatatacc ccgcccaatc ccagcgattc cgctgcggtt
    13081 aacgcattct gcgccatcat tgccgtatca acgacaccga gcagcaattg ttccgccagg
    13141 ccgagctgag catccggaca gatctgtaaa tggcggttaa agtcggcaca gaacacccag
    13201 aactccgccg cttgcgctac gtgtttttgc ccgccggtca gcgtcaccag ttcatcacgt
    13261 aacgctttgt cggtaatgcg aataatgcta ctgcactgca aaaaactgga actggacgtc
    13321 gcacgggcgc tgttaataat cgcctcacgc tgcgcttcgg aaatgggttc atcagtgaaa
    13381 tggcgaatgg agcgatggcc acaaataagt tcaatggttg gcgtcattat ctttttctct
    13441 ttctgaacgt gaatattgcg gtggacggtt catcagctgt ggggcaagac gttttgccac
    13501 ctgaagaata accaccaccg ctgtgggaag catgagcaaa acaccgagaa aaatcatcag
    13561 aatctgcact tctggccgag aaaatggctc aggcagcgac agggagccgc ttaccgacag
    13621 cagcgccacc gccagcaaca tcattccgat aaattccagt atcaacacgc ttttaggcaa
    13681 tttaccgatc gcgcgcatac gcttccctct gcaaagtgag ccttcagtct aaaacttttc
    13741 actgtattgt gtttaacagt tatagctttt agcaattaat gcaacaggtt aaacctactt
    13801 tcagcgaata cattttagcg tgatcattac aggcataaat ctatgaggag agaaataatg
    13861 caaaccgtta tttttggtcg tccgggttgc ccttactgtg tgcgtgcaaa agatctggct
    13921 gagaaattga gcaatgaacg cgatgatttt cagtatcagt atgttgatat tcgtgcggaa
    13981 gggatcacta aagaagatct acaacaaaag gcaggtaaac ccgtagaaac cgtgccgcag
    14041 atttttgtcg atcagcaaca tatcggcggc tataccgatt ttgctgcatg ggtgaaagag
    14101 aatctggacg cctgatcgtc tgacaagccc tcgcgttgag ggctttattg attttttctg
    14161 tgctgtggtt taaacaaact actgataaat aagaaacaca gtgcccccag cgcacaccag
    14221 aacaccgcac ttaataacca tgccagctct tgccagaatg agcgcgtcgg tgaaaaaaac
    14281 agccgcataa taagcattga acagggtgcc gccagcattg cgccaaacag aggtttcagg
    14341 acttctctac gctgtgaaaa gaagctggca actgctccag gaagaatgaa aaacagcaag
    14401 ccgatttcag gatgcccggc agcccgaaaa gcgcctttca tatgcgtcgc cagaaaaagg
    14461 cacaccacaa tgaagaggac aaaacagcag attgcccccg cccaacgttg tttatgtttc
    14521 actcgttcct cctgacactg cgtctatcga acacattttt cgccagtgtg gcgttcagta
    14581 agataaagcc gcttcgcatt ccatgctaat ataggccaac gcaattcata tagccgtt
//
LOCUS       DACBVI010000112        14592 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.112, whole genome shotgun sequence.
ACCESSION   DACBVI010000112 DACBVI010000000
VERSION     DACBVI010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.112"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(29..1483)
                     /gene="xylB"
                     /locus_tag="IU325_16490"
     CDS             complement(29..1483)
                     /gene="xylB"
                     /locus_tag="IU325_16490"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HAP5810599.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVAAQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRLLRPAILWNDGRCA
                     QECALLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDDMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAAVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLNNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLTDISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1555..2877)
                     /gene="xylA"
                     /locus_tag="IU325_16495"
     CDS             complement(1555..2877)
                     /gene="xylA"
                     /locus_tag="IU325_16495"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HAP5810600.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFHHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3243..4235
                     /gene="xylF"
                     /locus_tag="IU325_16500"
     CDS             3243..4235
                     /gene="xylF"
                     /locus_tag="IU325_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP5810601.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKETVVKDGFHKESEL"
     gene            4313..5854
                     /gene="xylG"
                     /locus_tag="IU325_16505"
     CDS             4313..5854
                     /gene="xylG"
                     /locus_tag="IU325_16505"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HAP5810602.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAVEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5832..7013
                     /gene="xylH"
                     /locus_tag="IU325_16510"
     CDS             5832..7013
                     /gene="xylH"
                     /locus_tag="IU325_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HAP5810603.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7091..8269
                     /locus_tag="IU325_16515"
     CDS             7091..8269
                     /locus_tag="IU325_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP5810604.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(8377..9201)
                     /locus_tag="IU325_16520"
     CDS             complement(8377..9201)
                     /locus_tag="IU325_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HAP5810605.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9521..11551
                     /gene="malS"
                     /locus_tag="IU325_16525"
     CDS             9521..11551
                     /gene="malS"
                     /locus_tag="IU325_16525"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAP5810606.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTINFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPT
                     LMISIQNAVEPVASLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            11729..12982
                     /gene="avtA"
                     /locus_tag="IU325_16530"
     CDS             11729..12982
                     /gene="avtA"
                     /locus_tag="IU325_16530"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HAP5810607.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLRDKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPDKIEAGVKILAEEIERAWAESH"
     gene            complement(13133..13606)
                     /locus_tag="IU325_16535"
     CDS             complement(13133..13606)
                     /locus_tag="IU325_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAP5810608.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(13708..14556)
                     /gene="yiaJ"
                     /locus_tag="IU325_16540"
     CDS             complement(13708..14556)
                     /gene="yiaJ"
                     /locus_tag="IU325_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HAP5810609.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWKSHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
BASE COUNT         3665 a         3731 c         3612 g         3584 t
ORIGIN      
        1 ccccacccgg tcaggcaggg gataacgttt acgccattaa tggcagaagt tgctgataga
       61 ggcgacggaa cgtttctcgt cgtggctgat aagcggcata acgctgcgca tctggtagat
      121 gcgactgttc taacggcagt tgtggcaaca actcaatgag cgatttctct ggattcgccg
      181 caatctgcgc cagccttgct gcgcccagtg ctggccccac atcccccccc gtgcggtaat
      241 cgagttgctg accgctgata tccgtcagca tctgacgcca gtactcacta cgcgccccgc
      301 caccaatcaa cgtaacactt tgcggtttaa tgccgcaggc atgcacgaca tccatgccat
      361 ctgccagcgc ataacccacg ccttccagca ctgctcgcgc cagttcattg gggccatgtt
      421 gatgagtcaa accaaagaaa acccccttcg cctggggatt attgtgtggt gtacgctcgc
      481 cggaaagata aggcagaaac caaactggct cggcactttc atcagcctgt tgagcagccg
      541 cgattaaagc tgggacattg ttcaggcccg ttaatttcgc agcccaatcc agacacgacg
      601 ctgcactcag catcacagac attaaatgcc aacgctgcgg tagcgcatgg caaaagctgt
      661 gtacggcgct ttctggcttg cttaagaacc cttcgctgac agcaaaatag actcccgacg
      721 tccccagcga taacattgcc tgattagcat caaccattcc tacaccaacc gcaccagctg
      781 cattatcacc accgcctgcg acaactggca ccgccgccat accccacgct ttcgcaactt
      841 caggtagcaa agcaccagta atttcgctgc cttcgtataa tgcgggcatc tggtcacgag
      901 ataagtggca agcctgcagc atgtcgtcac tccagtcacg ctttgcgaca tccagccaca
      961 tggtgccagc tgcgtcagac atatcgctgg caaactcccc cgtcatacgc agacgcaagt
     1021 aatctttcgg taacaatact ttgtcgattt gacggaatat ctccggctca tgccgctgaa
     1081 cccatagcaa tttaggcgca gtaaatccgg gcatcatcag gttaccggta atcacccgcg
     1141 attgcggaac tcgcgcttcc agcaaagcgc actcttgcgc acagcgcccg tcgttccaca
     1201 aaatggcagg gcgtaataac cgttgctggg catccagtaa ggttgcaccg tgcatctggc
     1261 cggcaatacc caatgcttta acgtcctgca gagaatgctg atcgcccagg gctttcattg
     1321 cgcgatcagt tgcctgccac cactgttccg ggtcttgttc cgaccagagt ggatgcgggc
     1381 gcgaaacggt cagcttttcc gtttgcgcag caaccacctc gccctgctcg ttgagcaaaa
     1441 taacttttac gcccgaggtg ccaagatcta tcccgatata catatcgatc gttccttaaa
     1501 aaaatgcccg gtatcgctac cgataaccgg gccaacggac tgcacagtta gccgttattt
     1561 gtcgaacaga taatggttta ccagattttc cagttgctcc tggcgaccac tctgatgcac
     1621 cggagacaaa ttgtgttcct gagcatattt ggctaaatct gccagtgaca tttggccttt
     1681 caggatttgc tggcctaatt cgctattcca gccggaataa cgctgcgcga tgcgtttatc
     1741 cagctcgcca tcttcaatca tgcgtgctgc aattttcagc gccaacgcca tcgtatccat
     1801 cgcgccgata tgaccgtaaa acagatcata tttatcagta ctttgacgac gtactttggc
     1861 atcgaagttc agaccgccgg tggtgaaacc gcctgctttg agaatttcat acatcaccaa
     1921 cgcattctct tccacactgt tcgggaactg gtcggtgtcc cagcccagtt gtgcatcgcc
     1981 acggttagcg tcgacagaac cgaacaggcc aagcgcaatg gcggtggcta tttcatgatg
     2041 gaaagagtga cctgccagcg tcgcgtggtt agcttcaatg ttcagtttaa tctctttttc
     2101 cagaccaaac tgtttcagga agccatagac tgtcgcagca tcgtaatcat attgatgttt
     2161 ggtcggttct tgcggtttcg gttcgataag caacgtaccc tgaaagccga ttttatgttt
     2221 atgctcaacc accatttgca taaagcggcc cagctgttca cgctcctgac gcaagtcggt
     2281 atttaacaac gtttcgtaac cttcacgacc gccccacagg acatagtttt caccgcccaa
     2341 tttatgggtt gcttccatcg ctgtaacaac ttgcgttgcc gcccagctga agacttctgg
     2401 atctgggttc gtcgccgcac ccgcgccgta gcgagggttt gtaaagcagt tagcggttcc
     2461 ccacagcagc ttcacgccgc tctcttcttg cttgcctgcc aggacatcaa ccatttgcgc
     2521 aaaattattg atgtactctt ttaacgacgc gccctcaggg gaaacatcca cgtcgtggaa
     2581 gcaataaaat ggcacatgta acttgtggaa aaactcaaat gcgacatctg ctttacgctt
     2641 cgccaacgcc agtgcctcac caggctgctg ccacggacga ttaaacgccc ccacaccaaa
     2701 catgtccgcc ccgttccagc agaaggtgtg ccagtagcag gcggcaaaac gcaagtgctc
     2761 ttccatacgc ttgcccaaca ccagttcgtc gggattatag tgatggaatg ctaacgggtt
     2821 agaggatttt gagccttcat aacgaacgcg atcgagctgg tcaaaatagg cttgcatatt
     2881 gaactccata atcaggtaat gccgcgggtg atggatgatg tcgtaatatt gggcactccc
     2941 tttcagttgc tcaattatgt tatttcacac tgctattgag ataattcaca agtgtgcgct
     3001 cgctcgcaaa ataaaatgga atgaggaaac tgggtaattc ctcgaagaga aaaatgcaat
     3061 aagtacaatt gcgcaacaaa agtaagatct cggtcataaa tcaagaaata aaccaaaaat
     3121 cgtaatcgaa agataaaaat ctgtaattgt tttcccctgt ttagttgcta aaaattggtt
     3181 acgtttatcg cggtgattgt tacttattaa agctgtcctc tacctacaga aggccctaca
     3241 ccatgaaaat aaagaacatt ctactcaccc tttgcacctc acttctgctt accaacgttg
     3301 cggcacacgc caaagaagtc aaaataggta tggcgattga tgatctccgt cttgaacgct
     3361 ggcaaaaaga tcgagatatt tttgtgaaaa aggcagaatc tctcggcgcg aaagtatttg
     3421 tacagtctgc aaatggcaat gaagaaacac aaatgtcgca gattgaaaac atgattaacc
     3481 ggggcgtcga tgttcttgtc attattccgt ataacggtca ggtattaagt aacgttgtaa
     3541 aagaagccaa acaagaaggt attaaagtat tagcttacga ccgtatgatt aacgatgcgg
     3601 atatcgattt ttatatttct ttcgataacg aaaaagtcgg tgaactgcag gcaaaagccc
     3661 tggtcgatat tgttccgcaa ggtaattact tcctgatggg cggctcgccg gtagataaca
     3721 acgccaagct gttccgcgcc gggcaaatga aagtgttaaa accttacgtt gattccggaa
     3781 aaattaaagt cgttggtgac caatgggttg atggctggtt accggaaaac gcattgaaaa
     3841 ttatggaaaa cgcgctaacc gccaataata acaaaattga tgctgtagtt gcctcaaacg
     3901 atgccaccgc aggtggggca attcaggcat taagtgcgca aggtttatca gggaaagtag
     3961 caatttccgg ccaggatgcg gatctcgcag gtattaaacg tattgctgcc ggtacgcaaa
     4021 ctatgacggt gtataaacct attacattgt tggcaaatac tgccgcagaa attgccgttg
     4081 agttgggcaa tggtcaggaa ccaaaagcag ataccactct gaataatggt ctgaaagatg
     4141 ttccctcccg cctgctgaca ccgatcgatg tgaataaaaa caacatcaaa gaaacggtag
     4201 ttaaagacgg attccacaaa gagagcgagc tgtaagcgtt acgccccggc ggggagcggg
     4261 ggcgtgattt ctctccatgc cgcgtgaatg aattggctta ggtggagtcg ttatgcctta
     4321 tctacttgaa atgaagaaca ttaccaaaac cttcggcagt gtgaaggcga ttgataacgt
     4381 cagcttgcgg ttgaatgctg gcgaaatcgt ctcactctgt ggggaaaatg ggtctggtaa
     4441 atcaacgctg atgaaagtgc tgtgtggtat ttatccccat ggctcctacg aaggcgaaat
     4501 tatttttgcg ggagaagaga ttcaggcgag tcacatccgc gataccgaac gcaaaggtat
     4561 cgccatcatt caccaggaat tggccctggt gaaagaattg actgtgctgg aaaatatctt
     4621 cctgggtaac gaaataaccc acaatggcat tatggattat gacctgatga cactacgctg
     4681 tcagaagctg ctcgcacagg tcagtttatc catttcaccc gatacccgcg ttggcgattt
     4741 agggcttggg caacaacaac tggttgaaat tgccaaggca cttaataaac aggtgcgttt
     4801 gttaattctc gatgaaccga cagcctcatt aactgagcag gaaacgtcgg ttttactgga
     4861 tattattcgc gatctgcaac agcacggtat cgcctgtatt tatatttcgc acaaactcaa
     4921 cgaagtcaaa gcgatttccg atacgatttg cgttattcgc gacggtcagc acattggtac
     4981 gcgtgatgct gccggaatga gtgaagacga tattatcacc atgatggtcg ggcgagagtt
     5041 aaccgcgctt taccctaatg aaccacatac caccggagat gaaatattac gtattgaaca
     5101 tctgacggca tggcatccgg ttaatcgtca tattaaacga gttaatgatg tctcgttttc
     5161 cctgaaacgt ggcgaaatac tgggtattgc cggactcgtt ggtgccggac gtaccgagac
     5221 cattcagtgc ctgtttggcg tgtggcccgg acaatgggaa ggaaaaattt atattgatgg
     5281 caaacaggta gatattcgta actgtcagca agccatcgcc caggggattg cgatggtccc
     5341 cgaagacaga aagcgcgacg gcatcgttcc ggtaatggcg gttggtaaaa atattaccct
     5401 cgccgcactc aataaattta ccggcggtat tagccagctt gatgacgcgg tagagcaaaa
     5461 atgtattctg gaatcaatcc agcaactcaa agttaaaacg tcgtcccccg accttgctat
     5521 tggacgtttg agcggcggca atcagcaaaa agcgatcctc gctcgctgtc tgttactcaa
     5581 cccgcgcatt ctcattcttg atgaacctac caggggtatc gatattggcg cgaaatacga
     5641 gatctacaaa ttaattaacc aactcgtcca gcagggtatt gccgttattg tcatctcttc
     5701 cgaattacct gaagtgctcg gccttagcga tcgtgtactg gtgatgcatg aagggaaact
     5761 aaaagccaac ctgataaatc ataacctgac tcaggagcag gtgatggaag ccgcattgag
     5821 gagcgaacat catgtcgaaa agcaatccgt ctgaagtgaa attggccgta ccgacatccg
     5881 gtggcttttc cgggttgaag tcactgaatt tgcaggtctt cgtgatgatt gcagctatca
     5941 tcgcaatcat gctgttcttt acctggacca ccgatggtgc ctacttaagc gctcgtaacg
     6001 tctccaacct gttacgccag accgcgatta ccggcatcct cgcggtagga atggtgttcg
     6061 tcataatttc tgctgaaatc gacctttccg tcggctcaat gatggggcta ttaggtggcg
     6121 tcgcggcgat ttgtgacgtc tggctaggct ggcctttgcc acttaccatc attgtgacgt
     6181 tggttctggg actgcttctc ggtgcctgga acggatggtg ggtcgcgtac cgcaaagtcc
     6241 cttcatttat tgtcaccctc gcgggcatgt tggcatttcg cggcatactc attggcatca
     6301 ccaacggcac gactgtttcc cccaccagcg ccgcgatgtc acaaatcggg caaagctatc
     6361 tacccgccag caccggcttc atcattggcg cgcttggctt aatggctttt gttggctggc
     6421 aatggcgcgg aagaatgcgc cgtcaggctt tgggtttgca gtctccggcc tctaccgcag
     6481 ttgtcggtcg ccaggcatta acggcgatta tcgtcttagg cgcaatctgg ctgttgaacg
     6541 attaccgtgg cgttcccact cctgttctgc tgctgacgtt gctgttactc ggcggaatgt
     6601 ttatggcaac gcggacggca tttggacgac gcatttatgc catcggcggc aatctggaag
     6661 cagcacgtct ctccgggatc aacgttgaac gcaccaaact tgccgtattc gctattaacg
     6721 gattaatggt cgctatcgcc ggattaatcc tcagttctcg acttggcgct ggttcacctt
     6781 ctgcgggaaa tatcgccgaa ctggacgcaa ttgccgcatg cgtgattggc ggcaccagtc
     6841 tggctggcgg tgtgggaagc gttgccggag cggtaatggg ggcattcatc atggcttcac
     6901 tggataacgg tatgagtatg atggatgtac cgaccttctg gcagtatatc gttaaaggtg
     6961 cgattctgtt gctggcagta tggatggact ccgcaaccaa acgccgttct tgattttgat
     7021 aaaaattttc tcaaagccgg taacgtatta ccggttttga gtttttgcat gattcagcag
     7081 gaaaagaacc atgtttacta aacgtcaccg catcacatta ctgttcaatg ccaataaagc
     7141 ctatgaccgg caggtagtag aaggcgtagg ggaatattta caggcgtcac aatcggaatg
     7201 ggatattttc attgaagaag atttccgcgc ccgcattgat aaaatcaagg actggttagg
     7261 agatggcgtc attgccgact tcgacgacaa acaaatcgag caagcgctgg ctgatgtcga
     7321 cgtccccatt gttggggttg gtggctcgta tcaccttgcc gaaagttacc cacccgttca
     7381 ttacattgcc accgataact atgcgctggt tgaaagcgca tttttgcatt taaaagagaa
     7441 aggcgttaac cgctttgctt tttatggtct tccggaatca agcggtaaac gttgggccac
     7501 tgaacgcgaa tatgcatttc gtcagcttgt cgccgaagaa aagtatcgcg gagtggttta
     7561 tcaggggtta gaaaccgcgc cagagaactg gcaacacgcg caaaatcggc tggcagactg
     7621 gctacaaacg ctgccaccgc aaaccgggat tattgccgtt actgacgccc gggcgcggca
     7681 tattctgcaa gtatgtgaac atctacatat tcccgtaccg gaaaaattat gcgtgattgg
     7741 catcgataac gaagaactga cccgctatct gtcgcgtgtc gccctttctt cggtcgctca
     7801 gggcgcgcgg caaatgggct atcaggcggc aaaactgttg catcgattat tagataaaga
     7861 agaaatgccg ctacagcgga ttttggtccc accagttcgc gtcattgaac ggcgctcaac
     7921 agattaccgc tcgctgaccg atcccgccgt tattcaggcc atgcattaca ttcgtaatca
     7981 cgcctgtaaa gggattaaag tggatcaggt actcgatgcg gtcgggatct cgcgctccaa
     8041 tcttgagaag cgttttaaag aagaggtggg cgaaaccatc catgccatga ttcatgccga
     8101 gaagttggag aaagcgcgca gtctgctgat ttcaaccacc ttgtcaatca atgagatatc
     8161 gcaaatgtgc ggttatccat cgctgcaata tttctactct gtttttaaaa aagcatatga
     8221 cacgacgcca aaagagtatc gcgatgtaaa tagcgaggtc atgttgtagc gatgatgaga
     8281 attgtcggcg tcgcatcagg taactcagca caaccacctg atgcgagcaa aagccttatc
     8341 cgactgacaa gtcggataag gctcgaggaa atgcaattac atatgcgccg cgattaaccg
     8401 ttggttatcc tggtacattg cgaacaggta gttgttataa ctcttccctt tggtcgagta
     8461 gcccttcagt ttgtgaatca tcgctgtggc agtcacttcc tgatccgctt tacgcagctg
     8521 tgcacgcgat ttacggaacg aagagtaagc cgggtgagta ttcaggttag tgacataggc
     8581 gctcaccgat tctttgacag aactaaactg tgagtacccc ttcactttac ccggcgcatt
     8641 agtacaacgt cctttcatgc atttcatgcc gaacaggttg ttgttgttgc gcgccagctt
     8701 cgacgttccc caaccgcttt ctgctgcagc catcgtcgcc accatactag tggggataat
     8761 gtctacgcgt tcaagcaagg tattccacgg gattttgcgc gtattaccag accacttcac
     8821 cttgtagcgt ttggcgatgt ctttcagacg cgcacgctca gcaggtgacc attggccctg
     8881 atactgtttt gaaatgagcc agttacgttc cgcagtaatg gccgcatttt ggctggtaat
     8941 gtaaggcatt acagtccgga gaaacgcctt tttccttggt gttccggaag ggtattttcg
     9001 caaatcagga agtgaactac tctttgcact attgcgagaa tactcttgtt tactgcttac
     9061 ctgtttatta ctagctttag ttaagtggga cttttgactc gctgttgttg tgtgcgtctt
     9121 cgctaacacc tcactcgaaa acaccagagt gagtaacata agaatcatcg ccccatatcg
     9181 tcgtatggga gtcaaaatca tcaggtctcc tggtcggatt taatcattcc aacaccttat
     9241 atttttcaca aatttgagag ttgaatctca aatcatatca aaaatagctg tcaagagcac
     9301 cccaaggaat agtccaaatc tgaaactatg tcacgagtta acgattcaga ttggcgctaa
     9361 atcgcagaaa atgtggggtt tatcgcaaaa ttcagccgtt ttttgcgcgc gatcgctcac
     9421 ccttgcttct catcctgtgg acttaccgct cagggatgag ttttgtctgg cttatcgctg
     9481 gcaaactgtc tgaaatcgca gcaataagga ctcatccgcc atgaaactcg ccgcctgttt
     9541 tctgacgctc cttcctggct tcgccgttgc cgccagctgg acttctccgg ggtttcccgc
     9601 ctttagcgaa caggggacgg gaacatttgt cagccacgcg cagttgccca aaggtacgcg
     9661 tccactaacg ataaattttg accaacagtg ctggcagcct gcggatgcga taaaactcaa
     9721 tcagatgctt tccctgcaac cttgtagcaa cacgccacct caatggcgat tgttcaggga
     9781 cggcgaatat acgctgcaac tagacacccg ctccggtaca ccaacattga tgatttccat
     9841 ccagaacgcc gtcgaaccgg tagcaagcct ggtccgtgaa tgcccgaaat gggatggatt
     9901 accgctcaca ctggatgtca gcgccacttt tccggaggga gccgcagtcc gggattatta
     9961 cagccagcaa attgcgatag tgaagaacgg tcaaataacg ttacaacccg ctgccaccag
    10021 caacggttta ctcctgctgg aacgggcaga aactgacgcc cctgcccctt tcgactggca
    10081 taacgccacg gtttactttg tgctgacaga tcgtttcgaa aacggcgatc ccagtaatga
    10141 ccagagttac ggacgtcata aagacggtat ggcagaaatt ggcacttttc acggcggcga
    10201 tttacgcggc ctgaccaaca aactggatta cctccagcag ctgggcgtca atgctttatg
    10261 gataagtgcc ccatttgagc aaattcacgg ctgggtcggc ggcggtacaa aaggcgattt
    10321 cccgcattat gcctaccacg gttattacac acaggactgg acgaatcttg atgccaatat
    10381 gggcagtgaa gccgatctac ggacactggt tgatagcgcg catcagcgcg gtattcgtat
    10441 tctctttgat gtcgtgatga accacaccgg ctatgccaca ctggcggata tgcaggagta
    10501 tcagtttggc gcgttatatc tttctggtga cgaagtgaaa aaaacgttgg gtgaacgctg
    10561 gagcgactgg aaacctgccg ccgggcaaac ctggcatagc tttaacgatt acattaattt
    10621 cagcgacaaa acaggctggg ataaatggtg gggaaaaaac tggatcagaa ccgatatcgg
    10681 cgattacgac aatccgggat tcgacgatct cactatgtcg ctggcctttt tgccggatat
    10741 caaaaccgaa tcaacgaccg cttctggtct gccggtgttc tataaaaaca aaacggatac
    10801 ccacgctaaa gtcatcgacg gctttacccc tcgcgattac ttaacccact ggttaagtca
    10861 gtgggtccgc gactatggga ttgatggttt tcgggtcgac accgccaaac atgttgagtt
    10921 gcccgcctgg cagcaactga aaaccgaagc cagcgccgcg cttcgcgaat ggaaaaaagc
    10981 taaccccgac aaagcattag atgacaaacc tttctggatg accggtgaag cctggggcca
    11041 cggcgtgatg caaagtgact actatcgcca cggcttcgat gcgatgatca atttcgatta
    11101 tcaggagcag gcggctaaag cagtcgattg tctggcgcag atggatacga cctggcagca
    11161 aatggcggag aaattgcagg atttcaacgt gttgagctac ctctcgtcgc atgatacccg
    11221 cctgttccgt gaagggggcg acaaagcagc agagttatta ttattagcgc caggcgcggt
    11281 acaaatcttt tatggtgatg aatcctcgcg tccgttcggc cccaccggtt cggatccgct
    11341 gcaaggtacg cgttcggata tgaactggca ggatgttagc ggtaaatctg ccgccagcgt
    11401 cgcgcactgg cagaaaatca gccagttccg cgcccgccat cccgcaattg gcgcgggcaa
    11461 acaaacgaca ctttcgctga agcagggcta cggctttgtt cgtgagcatg gcgacgataa
    11521 agtgctggtc atctgggctg ggcaacagtg acttttccgg cttcccgttc gtcagtacct
    11581 cgggaagccg ccaaccagga taaaatgtca gagctaatca gcgttgcagg ataaagcacc
    11641 gctcactctt caacagagcg atttgcaccc cagcaaatgt agcgttattg ttaccttcct
    11701 tgctacagag ttcgacagat atcccgctat gacattctcc ctttttggtg acaaatttac
    11761 ccgccactcc ggcattacgc tgctgatgga agatctgaac gacggtttac gcacgcctgg
    11821 cgcgattatg ctcggcggcg gtaatccagc gcagatcccg gaaatgcagg actacttcca
    11881 gacgctactg accgacatgc tggaaagtgg caaagcgact gatgcactgt gcaattacga
    11941 cggtccacag gggaaaacgg agctactcac actgcttgcc ggaatgctgc gcgataagtt
    12001 gggttgggat atcgaaccac agaatattgc actaacaaac ggcagccaga gcgcgttttt
    12061 ctacttattt aatctgttcg ccggacgccg tgccgatggt cgggtcaaaa aagtgctgtt
    12121 tccgctggca ccggagtaca ttggctacgc tgacgccgga ctggaagaag atctgtttgt
    12181 ctctgcgcgt ccgaatattg aactgctgcc ggaaggccag tttaaatacc acgtcgattt
    12241 tgagcatctg catattggcg aagaaaccgg gatgatttgc gtctcccggc cgacgaatcc
    12301 aacaggcaat gtgattactg acgaagagtt gctgaagctt gacgcgctgg cgaatcaaca
    12361 tggcattccg ctggtgattg ataacgctta tggcgtcccg ttcccgggta tcatcttcag
    12421 cgaagcgcgc ccgctgtgga atccgaatat cgtactgtgc atgagtcttt ccaagctggg
    12481 tctacctggc tcccgctgcg gcattatcat cgccaatgaa aaaatcatca ccgccatcac
    12541 caatatgaac ggcattatca gcctggcacc tggcggtatt ggtccggcga tgatgtgtga
    12601 aatgattaag cgtaacgatc tgctgcgcct gtctgaaaca gtcatcaaac cgttttacta
    12661 ccagcgtgtt caggaaacta tcgccatcat tcgccgctat ttaccggaag atcgctgcct
    12721 gattcataaa ccggaaggag ccattttcct ctggctatgg tttaaggatt tgcccattac
    12781 gaccgagcag ctctatcagc gcctgaaagc acgcggcgtg ctgatggtgc cggggcacaa
    12841 cttcttccca gggctggata aaccgtggcc acatacacat caatgtatgc gcatgaacta
    12901 cgtgccggag ccggataaaa ttgaggcggg tgtgaagatt ctggcagaag agatagaaag
    12961 agcctgggct gaaagtcact aaaaattgcc tgatgcgcta cgcttatcag gcctacatga
    13021 tttctgcaat atattgaatt tggtagaatt tgtaggccgg ataaggcgtt ttagccgcat
    13081 ccggcataaa caaagcgcac tattccggcg ttgagaaacg ccggaaaacg tttcaaacag
    13141 gctgctgccg tagccgatgc cgctgcactt tctcgacatc catgcactgc aacgcctttg
    13201 tcgggcaggc ttcaacacag gccggccccg tctcccgatg ccagcacaga tcgcacttaa
    13261 tcgccctcgc ctttttgcgc gaagaaacga cctccatcgc accaaacggg caagccagca
    13321 tacagctttt acagccaatg caacgtgatt gttcaacgaa aatatgccca tgttcgcggc
    13381 ttatcgcgtc aacagggcag acattcgcgc acggtgcatc ttcacactga tgacaggcta
    13441 ccgccgtggt ccagctgtgg tctttaatga cacgaatacg ggaaataaac tcgtctggtg
    13501 acaacgcagc gcaatcctga ttctcctgat gcgacactgc gcaagccact tcacaggtac
    13561 ggcaaccgat acatttcgtc gcatccgcaa taataaaccg gttcatgatc actcctttgc
    13621 actaaaacag cagagtgcca gagagcgcgt ctctactgct ttgatccaac gaaggaaatg
    13681 ttaaagtttt ggcacttgtc caaagcgtta tgtgatcgcg ccctggtcgt ccctgacagt
    13741 aaatcccagt tcattagaaa tagcctgcgc ggtttcacgc agtggtttca ggagattttt
    13801 ctcccccacc tgtttcagac gtgatgtcga aagagaaatc gacacggcgt acggcacccg
    13861 cccatgaata tcaaacaccg gaacagcaat acaggagacg ccgagttcgt tttcttctct
    13921 gtccatcgcc gctccgcttt cacgaatgtg cgccagttcg tcgaacatcg cgggcagctc
    13981 ggtaatggta ttgcgggtta acggctggat ctcatgctga tggcttttcc agtatgactt
    14041 cacgtagtcc gggtgaccaa acgccatgta gatcttgccc attgcggaac agtagagcgg
    14101 catatgctgg cctatatagg cacgggttcg cagcatcccg gttgtgggtt ccagcttata
    14161 aatcaaaata gcgtgatcgt cttcgcggct ggagaagtta atggtttcac cagtggcgat
    14221 gttcagtgcc tcaagatgcg gagcggcgat atgaatgata ttcagcgaag acagcgcctt
    14281 ctgcccgacg gcaataaatt tggtagtcag gcggtaactc cctgcggcgg gcgcggtggt
    14341 cacatagccg caggactgta atccctgcaa taagcgatgg acggtactct tatttaaacc
    14401 agccagctcc gaaagatgcg ccaacggaca accgtttgga tagttgctca aaatctcaat
    14461 cagcatcaac ccgcgaaaca gactctggct tccggctgga cgctcttttt cctgcgccat
    14521 ctcgttctct ttttttccca tcacttcttt ccccattttg tcgcgtcctg atggtagcgc
    14581 aaagtgtgtc gt
//
LOCUS       DACBVI010000113        14506 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.113, whole genome shotgun sequence.
ACCESSION   DACBVI010000113 DACBVI010000000
VERSION     DACBVI010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.113"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            182..724
                     /locus_tag="IU325_16545"
     CDS             182..724
                     /locus_tag="IU325_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810610.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDAAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWQIGDSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            824..2026
                     /locus_tag="IU325_16550"
     CDS             824..2026
                     /locus_tag="IU325_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HAP5810611.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATARGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TSGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2246..3028
                     /locus_tag="IU325_16555"
     CDS             2246..3028
                     /locus_tag="IU325_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP5810612.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTKLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEEL
                     VWEQMTPTTITHPQRLYEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            3043..4248
                     /gene="rhmD"
                     /locus_tag="IU325_16560"
     CDS             3043..4248
                     /gene="rhmD"
                     /locus_tag="IU325_16560"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HAP5810613.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIHKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4305..5594
                     /locus_tag="IU325_16565"
     CDS             4305..5594
                     /locus_tag="IU325_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5810614.1"
                     /translation="MSTALLDAVVKKNRARLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKMGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            5611..6414
                     /locus_tag="IU325_16570"
     CDS             5611..6414
                     /locus_tag="IU325_16570"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HAP5810615.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(6455..7357)
                     /locus_tag="IU325_16575"
     CDS             complement(6455..7357)
                     /locus_tag="IU325_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAP5810616.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMREQAIKIALRMLE
                     QGIDRDLVLAATQLSEADLAANNH"
     gene            complement(7550..8740)
                     /gene="glpC"
                     /locus_tag="IU325_16580"
     CDS             complement(7550..8740)
                     /gene="glpC"
                     /locus_tag="IU325_16580"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HAP5810617.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(8737..9996)
                     /gene="glpB"
                     /locus_tag="IU325_16585"
     CDS             complement(8737..9996)
                     /gene="glpB"
                     /locus_tag="IU325_16585"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HAP5810618.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDSQAVTDIHHGLESLCQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELGLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERDGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQLFGVTTDEALRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(9986..11614)
                     /gene="glpA"
                     /locus_tag="IU325_16590"
     CDS             complement(9986..11614)
                     /gene="glpA"
                     /locus_tag="IU325_16590"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HAP5810619.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGEIQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYS
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADTPLPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            11887..13245
                     /gene="glpT"
                     /locus_tag="IU325_16595"
     CDS             11887..13245
                     /gene="glpT"
                     /locus_tag="IU325_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HAP5810620.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            13250..14326
                     /gene="glpQ"
                     /locus_tag="IU325_16600"
     CDS             13250..14326
                     /gene="glpQ"
                     /locus_tag="IU325_16600"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP5810621.1"
                     /translation="MKLTLKNLSMAIMMSGMIMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYSGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
BASE COUNT         3286 a         3593 c         4002 g         3625 t
ORIGIN      
        1 tattattatt tttgtgtgta tcgataaagt gtgtttattt tttcggcgtt taattattta
       61 agaagtattt agttacaata agtgactaga tccctttttt cgctcattga atcctctgcg
      121 ccaggcagta caatcccgca gctttgggaa ttttgattaa ttgcttgtgg gaaagaggat
      181 aatgaaaaaa attgcgctag caggtctggc cggaatgctg ttggtttctg catcggtcaa
      241 tgcaatgagc atcagcggcc aggcgggtaa agaatacacc aatattggtg tcggttttgg
      301 gactgaatcg acgggcctgg cgttaagcgg taactggaca cataacgacg acgacggtga
      361 tgccgcgggc gtggggctgg ggttgaatct gcctctcggg ccattaatgg cgaccgttgg
      421 cggaaaaggc gtttacacca acccgaatta cggtgatgaa ggttatgcag cggcagttgg
      481 cggtggtttg cagtggcaaa ttggtgacag cttccgcttg tttggtgagt attactactc
      541 tccggattcg ctctccagcg gtattcaaag ttatgaggaa gcgaatgctg gcgcgcgtta
      601 caccattatg cgtccagtca gcattgaggc gggttatcgc tacctgaatc tgtcgggtaa
      661 agatggtaac cgcgacaacg ccgtggctga cggcccgtat gttggggtta acgccagttt
      721 ctgatccctt ccggcgcggc attctgccgc gccattcagt ttcttactac gccttatctc
      781 cctttgcgtt atagtgattc gataacaaaa gcaggagata accatgttaa aagtggaaat
      841 gctatccacc ggggatgaag tgttacacgg gcaaatcgtt gacactaacg ctgcctggct
      901 ggccgatttt ttctttcatc aggggttgcc attatctcgc cgcaatacgg tgggggataa
      961 ccttgatgac ttagtgacca ttcttcgcga acggagtcag cacgccgatg tgctgatcgt
     1021 taacggcggg ctggggccga ccagtgatga tttaagcgca ctcgccgctg cgacagcaag
     1081 aggtgaaggc ctggtgttgc atgaagcctg gctcaaagag atggaacgct atttccacga
     1141 acgtggacga gtaatggcac cgagcaaccg taaacaagcg gagctacctg ccagtgctga
     1201 atttatcaat aacccggtag gcaccgcctg tggttttgcc gtgcagctta atcgttgcct
     1261 gatgttcttt acccccggcg taccgtcaga atttaaggtg atggtcgagc acgaaatcct
     1321 gccgcgtctg cgcgagcgtt tttctttacc acagccgccg gtttgtctgc gtttaactac
     1381 ctttggtcgt tcggaaagcg atctggcaca aagcctggac actctacaac tgccgccggg
     1441 cgtaacaatg ggctatcgct cctcaatgcc tatcatcgaa ctgaaactca ccggaccggc
     1501 aagcgagcaa caggcgatgg aaaaactgtg gctggacgtt aaacgtgttg ccggacagag
     1561 cgtgattttc gaaggcaccg aaggactgcc cgcgcagatc agtcgcgaat tgcaaaaccg
     1621 ccagttcagc ctgacgttga gcgagcaatt caccagcggt ttattggctt tgcaactttc
     1681 tcgcgcaggt gctccattgc tggcgtgtga agtggttcct tcacaggaag aaaccctggc
     1741 gcaaactgcg cactggatta cagaacggcg ggccaaccat tttgccgggc tggcactggc
     1801 tgtttcgggt ttcgagaacg agcatctcaa ctttgcgcta gccacgccag atggcacttt
     1861 cgctctgcgt gtgcgtttca gcactacgcg ctacagcctg gctatccgtc aggaagtgtg
     1921 cgcaatgatg gcactgaata tgctgcgccg ttggttaaac ggccaggaca ttgccagtga
     1981 gcatggctgg attgaggttg ttgagtccat gaccttatcc gtctgattcc gccactctgt
     2041 gccggtttac tgcaccggca cattccccct ccggcaaaat ttgtccaacc tgttgtactc
     2101 aataagaaaa aatccgtgcg ctgattcaca aatttgtgcg taatttctcc agtccgctgg
     2161 acgaggtgcg ggatttgatg cgcttcacaa ttttactccc cctgagcaac gagactgatt
     2221 ccagtatcta agactggagt ccagtatgtt ggaatcaagc aaagttcccg ccctaacacg
     2281 ggctattgat attctcaatt tgattgcccg cattggcccc tgtagtgctg cgacgatcat
     2341 tgacacattg gggatcccta aaagcacggc ctatttgctg cttaatgaac tcaggcgtca
     2401 gcgttttctt agccttgatc accaggaaaa tttttgtttg tggaccaagc tggtggagct
     2461 ttccggtcat gcgttaagca agatggatct ccgggaactg gctcgaccgc gtttgacgca
     2521 actgatggat accaccgggc tgttatgtca cctggggatt attgataacg gcagcgctta
     2581 ttacattttg aaagtggagt catcagccac tatcagcgtg cgttcccatg aaggaaaaag
     2641 cctttcgctt tatcgctccg gaataggtaa atgcctgctt gcctggcaac ctgcagctgt
     2701 acagcaaagt attatcgaag agctggtctg ggagcagatg acacccacca ccattaccca
     2761 tccacaacgg ctctacgagg aactggcgcg tattcgccgt cagggctgga gttatgacaa
     2821 cggcgaagat tatgctgatg tccgttgtgt cgctgcgccg gtgtttaacg ccaataacga
     2881 gcttaccgct gcgatttcgg tggtcggtac ccgcttacaa atcaatgaag aataccgtga
     2941 ttatctggcg ggcaaggcca ttgcctgcgc cagggatatt tcacgtctgt tgggatggaa
     3001 aagtcccttt gacttacagg cctcataaca atggagaaca ttatgaccct accaaaaatc
     3061 aaacaggttc gcgcctggtt tactggcggt gcgacagcag aaaaaggcgc tggcggcggt
     3121 gattatcacg accagggggc aaatcactgg attgatgatc atattgccac cccgatgagt
     3181 aaataccgcg attacgagca gtcacgccag tcatttggca ttaacgttct tggcacgttg
     3241 attgttgaag tcgaagcaga aaacggccag accggattcg ccgtttcgac agccggtgaa
     3301 atgggctgtt ttattgtcga aaaacatctt aaccgtttca ttgagggtaa atgtgtcagt
     3361 gatatcaaac tgatccacga tcaaatgctc aatgccaccc tgtattactc cggctctggt
     3421 ggcctggtga tgaacacgat ttcctgtgtc gatctggctc tgtgggatct gttcggcaaa
     3481 gtggtcgggc ttccggttta taaactttta ggcggcgcag ttcgtgatga gattcagttc
     3541 tacgccacag gtgcgcgtcc ggatctggca aaagagatgg gctttatcgg tggcaaaatg
     3601 ccgacgcact gggggccaca tgatggcgat gcggggatcc acaaagatgc cgctatggtc
     3661 gcggatatgc gtgaaaaatg cggtgaggat ttctggttaa tgctcgactg ctggatgagt
     3721 caggacgtga actatgccac caaactggcc cacgcttgcg cgccctataa cctgaaatgg
     3781 atcgaagagt gcctgccgcc acagcagtat gaaggttatc gcgaactgaa acgcaacgcg
     3841 ccagtcggaa tgatggtcac cagcggtgag catcacggca cactgcaatc tttccgtacg
     3901 ctttcagaaa ccggtatcga cattatgcag ccggatgttg gctggtgcgg tggcttaacc
     3961 acgctggtgg aaattgccgc aatcgccaaa tccagagggc aactggtggt gccgcacggt
     4021 tcgtctgttt actctcacca tgcggtgatc accttcacca atacgccatt cagcgaattc
     4081 ctgatgacca gcccggattg ttcaacgatg cgtccgcaat ttgatccgat tctgcttaat
     4141 gagccagtgc cggtgaatgg tcgtattcat aaatcagtgc ttgataaacc cggtttcggc
     4201 gtcgaactca atcgtgactg caatttgaaa cgcccctaca gccactaatc actccgggcg
     4261 ttgctgtgac aacggtaacg cccacatatt ctaaggagct ttttatgagc accgctttgc
     4321 ttgacgccgt ggtgaagaaa aaccgagcgc gtttaattcc gtttatgttg gcgctgtatg
     4381 tgctggcatt tctcgaccgt tcgaatattg gttttgccaa acaaacctac cagattgata
     4441 ccgggctgag taatgaagct tatgcgctgg gagcaggcat tttctttgtg gtatatgcgt
     4501 ttctgggtgt tccggcgaat cttttgatgc gcaaaatggg ggccagaacc tggattggta
     4561 cgacaacact gctgtgggga tttctttcgg cagccatggc atgggccgat actgaagcga
     4621 aatttctgat cgttcgtact ctgcttggtg ctgcggaggc cgggttcttc cctggtatga
     4681 tttatctcac ctcgcaatgg tttccgcagc gtaatcgcgc cagcattatg gggttgttct
     4741 atatgggcgc accgctggcg ttaacactgg gatcaccgct ttccggcgcg ctgctggaga
     4801 tgcatggatt tatggggcat cccggctggt tctggatgtt tgtgattgaa ggattgctgg
     4861 cagttggcgc tggggtattc acattctttt ggcttgatga cacaccggag caggcacgtt
     4921 ttctgagtaa acaagaaaaa acgttgctta tcaatcaact ggcaagtgaa gaacaacaga
     4981 aagtgacttc tcgattgagc gatgcgctgc gtaatggccg agtctggcaa ctggcgatta
     5041 tctacctgac cattcaggtg gcggtttacg gattaatttt cttcctgccg acccaggttg
     5101 ctgcattgct ggggacaaaa gtgggcttta cggcgtcggt ggtcaccgcc attccgtggg
     5161 tcgcggcctt gtttgggacc tggcttattc cgcgctattc cgataaaacc ggcgaacggc
     5221 gtaatgtcgc ggcgctgaca ttactggcgg cgggcattgg tattggtctc tccgggctgc
     5281 tttctccagt actggcgatc gtagcgctgt gtgttgcagc catcgggttt attgccgtgc
     5341 agccggtgtt ctggacgatg ccgacacaac tgctttccgg tacggcgctg gcggcgggga
     5401 ttggttttgt aaacctgttt ggtgcagtgg gcgggtttat tgccccgatc ctgcgcgtga
     5461 aagcagaaac gttgtttgcc agcgatgcgg cgggattact gacgctggca gcggtggcgg
     5521 tcatcggttc gctgattatt ttcactctgc gtgtaaatcg cactgttgcg cagaccgacg
     5581 tggcacatca ttaataggtt aaggaacacc atgaacgcat tattaaccaa tccctttaaa
     5641 gaacgtttac gcaaaggcga agtgcaaatt ggtctgtggt taagctcgac tacttcttat
     5701 atggcagaaa ttgccgccac ctccggttat gactggttgc tgattgacgg ggagcacgcg
     5761 ccaaacacca ttcaggatct ttatcaccag ctacaggcgg tagcgcccta tgccagccaa
     5821 cccgtgatcc gtccggtgga aggcagtaag tcgctgatta aacaagtcct ggatattggc
     5881 gcgcaaactc tactaatccc gatggtcgat actgccgatc aggcacgtca ggtggtgtct
     5941 gccacgcgct atcctcccta cggtgagcgt ggtgtcgggg ccagtgtggc acgggctgcg
     6001 cgctggggac gcattgagaa ttacatggcg caagtcaacg attcgctttg tctgttggtg
     6061 caggtggaaa gtaaaacagc actggataac ctggacgaaa tcctcgacgt cgaagggatt
     6121 gacggcgtgt ttattggacc tgcggatctt tctgcctcgt tgggctaccc ggataacgcc
     6181 gggcacccgg aagtgcagcg aattattgaa accagcattc ggcggatccg tgctgcgggt
     6241 aaagcggctg gttttctggc tgtggctcct gatatggcgc agcaatgcct ggcgtgggga
     6301 gcgaactttg tcgctgttgg tgttgacacg atgctctaca gcgatgccct ggatcaacga
     6361 ctggcgatgt ttaaatcagg caaaaatggg ccacgcataa aaggtagtta ttgatatcaa
     6421 aggcccatgg ggatcggctg tgggcctgtg ttaattagtg gttattcgct gccagatcgg
     6481 cttcgcttag ctgggtggcc gcgagcacca ggtcacgatc aatgccctgt tccagcatgc
     6541 gcaaggcaat tttaatggct tgttcacgca taccttcctg ccagccaatt tggtgtcctt
     6601 cctgccgtag ccgttcagca atagtcatta atctctcctt ttgtagtggt gaacgttcgg
     6661 caatctcctc aataaaacgg gtgaaacggg aggtatcgcc gcattgcagc agataattaa
     6721 acagcgtttg tagctggctg tcattagtga agcctctaac caaaagcgtg gtgatcctgt
     6781 cgaccatacc gattaaatcg cggtcgcgaa tatgcttttg aatcagttca agcagagcta
     6841 tacgccgatg ttgcataatc tcatcgtcag gtacgatggt gatatccacc aacggaaaag
     6901 cttcggtgta caactgccgg gcaagttgcg gatcgtcaaa ctcatccagc cagttgagtg
     6961 agtacgggta gggcgaggtt tcgccatgat aaaacagcaa tggcaccacc agcggaactc
     7021 tgtcatagcc tttatccagg tgacgctgca tggcggcagt ggcatagcgc attagccgaa
     7081 aagccatatt cttttctgca gagctttgat gttcaatcac gcagtagata taaccgtcac
     7141 cgtcgctggt ttccacggac cacaaaacat ccgagtagta agcgcgtaaa cttttttcaa
     7201 taaaactggt gggttccagg cgtaaggttt gcaggttgca aagcttgcgc agcggctctg
     7261 gtagatgtat ttccagaaaa tcccgtgcgg tttcgggtgt gaacataaag gttttaaaca
     7321 ccgcatcatg cggggaggag gttgttgatt cggtcatggt tgtccgtcac ctgatattta
     7381 tgatgacgcg acattacggt tatcccaggc ctttgacatc tgccagtttt agcctttgcg
     7441 agacgttttc agaggaattt tcactgcgtt gcaacgcata cattgggcac ggaatcgtgt
     7501 acgcaaaaat gcccggtaac aacatgctta ccgggcatca gaaaggagtt taagccagcg
     7561 cctgggccag cagcgtaatc ggatgttcgc agcgaagact tgtggacatc tcaatctgcc
     7621 atttgcaggt ttcgcagtcg gtaaccacca gatctgcgcc gctttcttct atctgacgga
     7681 acagtggtgc gccgatggct tgtgaggtgg ggtagttctc ttttttgaaa ccgtaagtac
     7741 ctgcaatacc gcagcactgg gaatccagca ccgttaactc aagtcccggg attttacgca
     7801 acagctccag ggtgtagagc gtccagccca ttttttccat atggcacgga gtgtgataaa
     7861 ccactttcag cggcagcggt ttcagcggta acgttttgcc ttcgtccagc ttgcgccaca
     7921 gccagcgggt cgccagttcg atgtgatcgc gcaagccttt gttgtcgaca ttcagcactt
     7981 ctgggtattc gtcgcgcagg gcaaatgtac aggttgagga ggtggcaatc accggaatgc
     8041 cttttactcc cactgcttcg cggatcgact ctacatttgt gattgcctgt ttacgtgctt
     8101 tatcggtaaa gccgttggcg atcagcggta cgccgcagca tttttctttg ctgagcagtt
     8161 gtacaccggt acccattgcg ttgagcactt taattaaatc tttgcctaac tgcggatggt
     8221 tgtagttaac gaagcagccg tgaaagaaag ccacctggtc tttatattgc gcttgttggg
     8281 ctgccacgct gcgataccag cgacggaacg taccgaagga gtatttcggt agcgtgcggc
     8341 gatgatcgat ttttaacgcc gcatcaagca gctgccgcac tggtttcagc gaggtagcgg
     8401 tattgacgat tggtgcgaat ggcgtggaaa cgctacccat caggtcggta tgactcaaca
     8461 caaaattgcg cagcgacggg cgtgtggtgt catatttcgc ccgtgcgcgc tggatgatat
     8521 cgccaatctt cacatcggac gggcaggcga cttcgcaacg tttgcagttg atgcaatatt
     8581 tcagtgcctc gtcatacagt gcgccatcct tcaaacgcag acgctcgcca tccggcccgg
     8641 cttgttttgg ccccggatat cccggattca cccggctcac cgggcaggcg gtggtgcaga
     8701 cggtgcactt aatgcagttt tcgaagctgg tgtcgttcat tgttgacctc ctgcgcgttg
     8761 ggcaatctgt tgagcggcat gtaaagcact gacggcacaa acaccgccgc cgcatccctg
     8821 ggcgatggga tcaaatccgc ccagcaccga accgatggca aacaggttct caatggtttg
     8881 ccctgcctgc gacgggcgta gtgcctcatc agtggttaca ccgaacagct gccacggttg
     8941 cggcgcaaaa aaatcgccct tataccattc accccgcgtg gcggtttgta gcacatcaag
     9001 gccgagaatc ggctcgcgaa tgccgtcacg ttctgccacc agtccgccgc taaagaaact
     9061 gccgctcgca agcaccgcaa atcgtgggcg cagcgggata tcggcgtgat tgcgcgtcca
     9121 gatttcattc actacgccat ttttacaggt cacttttttc acttcatcgc ctggcatcca
     9181 cacgccaccc tggcgcacaa actggcgctg taactggttt tgcagacgaa tgcccagcac
     9241 ggaaggcggc agcgttggca aaagcatcag tgaacaaggt agtttttcat tcaaccaacg
     9301 ccacagtttg tcatcggcca ggccgaagca ggcgggcatc aggatcattt cgcaggtatt
     9361 ggcgacagga ataagcgcat caagtaacag cggccagttt tcttcattat caaggaaacg
     9421 ggcgatattc accgcgcgga actcggtggc gttattgcgc aggacatcca gttccggcag
     9481 ctctatttct gcggtttcaa cggcaaggcc gagttcacgc aacgaagctg ccgcaaggtg
     9541 agcctgaaaa tccatcaggc cgctaattcc cactacacat attttcttcg cgggcagtgg
     9601 ccagacgggg acttctggcg aacttagcca ggtagaacgc agagtgccga gcggcgtaat
     9661 ccgctggtga gccagttcta cgctgccctg caattgcgca ccgctttcag cgattaatgc
     9721 ctgcgcctgg caagcgagat cgagtacgcg ttgtggcccg agaagagagt aaggatgggc
     9781 aggggcctgc tgacataacg attccagccc gtggtgaatg tctgtcacag cttgactatc
     9841 tggcagatgg ctcagcaaat ccagcgaacc ggatgagaaa tgcagcgcgc tttgaccacg
     9901 cgtgacaatg gcacagcgca ggccgtgttt ttgcagttgc aggccacaga gtaagccggc
     9961 gaggccgccg cccataatga cagtatcaaa gcgcatcttt ctgctccttc tccagaccac
    10021 acagtccctg ataaacccag cgggtaaatt cgctttcgcg cagtgcgtct ccccatgcta
    10081 tgggttgcac gcctttccag cgttcgttaa ggaaggtgga aagttgctcg atagattgcg
    10141 cggacgtcgt gacgttaaaa cgttgcagca gtccggcagc ccggcaggcg cagagttcgc
    10201 cctggcaggt acccatccct acacgggtac gacggcgtaa atccagcagg ctgttaacgt
    10261 ttaaattttc tactgcgtac tgcacttcac ccgcagttac cgcttcgcac tcacatacca
    10321 ggctgcggtg caggcggcct tcgctcagcc aggctggtgt gcgatcgccg tgccggtaaa
    10381 ctgcggaacc gcgcagcgga gcgggcaggg agatcacttt gcgcagcgtg acttctgccg
    10441 gttcctgtga gccaggcaac ggcgtgtcgg cagtggtaca ggggcgcgtg ttgcccagct
    10501 tgcgacacac cgcgtcagta gcccattccg ccatcagccg ataggtcatc agtttgccac
    10561 cggtgatggt gataaatccg tcgagaccat cgcgttcagc atggtcgagc agcacgatgc
    10621 cacggctgac gttacgtccg ctcgggtcgt catcgctggc aaccagcgga cgcacgccag
    10681 aataggcccg caaaatgcgt gttttcgcca tcacgggggc cagtttttcc ccttcacgca
    10741 gcagaatatc aacttcttct gccgtcactc gattatcgtc aatctcgctg taatcaatac
    10801 gtaaagaggt ggtgccaatc agcgaaatgg tatcgccagg caccagaata tcggcgtcgg
    10861 aaggtttgcg gcagcggttg atcacatgct gattaatgcg gtgatccata atcagcagcg
    10921 atcctttcgc cgggaacatg cgaatgcgca gatcggcata ttcggcaatg tgttgccccc
    10981 aaatcccggc ggcattaact acgacaggcg catgaagtgc ctgaatttcg ccggtgagat
    11041 ggttacgtac acgaacaccg cacaccgtcg cgccttcacg aatcagcccc gtgacttcat
    11101 gagcggtaag gataaccgca ccgtgttctt tggcatcgag catgttcgct gcggtcagac
    11161 gaaatggatc aacggtgcca tccggaactt tcaccgcgcc aatcagtgcc gggttaacgg
    11221 caggttcgat aatgcgcgct tgctgcgggt ctatagcttc tgcgctgatc cctgcttctt
    11281 cgcaggcgcg aataaaagtg gcctggaagg agaggtcatc ttccggcagg gtgataaaca
    11341 ggccgttggt cggttcaacg cagtgacgtg caatgcgttt caggatctgg ttttcactaa
    11401 tacattcgcg ggccgattcc gcatcggtta ccgcatagcg cgcaccactg tgcagcaggc
    11461 cgtggttacg cccggtggca ccggttgcga tgtcgtggcg ctcaaccaaa atcacgcgca
    11521 gcccgcgcag ggcacagtcg cgggcaatcc cggctcccgt tgcgccgccg ccaatgataa
    11581 tcacgtcact tgattgcgag tcgcgagttt tcattgttat ccctctgaag ttcgtttttt
    11641 accatttagc catagtaaaa acatgaattg tttgatttcg cgcatattcg ctcataattc
    11701 gaaagtgaaa cgtgatttca tgcgtcattc tgaacatttc aaccatcgta tttaataatg
    11761 tgtgcggtaa ttcacattta atttatgaat gttttcttaa catcgcggca actcaagaaa
    11821 cggcaggttc tcccactgaa tcaggctgtt aatcataaat aagaccacgg gccacggagg
    11881 ctatcaatgt tgagtatttt taaaccagcg ccacataaag cgcgcttacc tgccgcggag
    11941 atcgatccga cttatcgtcg attgcgctgg caaattttcc tggggatatt ctttggctat
    12001 gctgcttact atttggttcg taagaacttt gcgctcgcta tgccttatct ggttgagcag
    12061 ggattctcac gcggtgattt aggttttgcc ctttcgggga tctcgattgc ttatggattt
    12121 tcgaaattca tcatgggttc ggtatcggat cgctcgaatc cgcgcgtttt cctgcccgca
    12181 ggtttgattc tggcggcggc agtgatgttg tttatgggct ttgtgccatg ggcgacgtcg
    12241 agcattgcgg tgatgtttgt gctgctgttc ctctgcggtt ggttccaggg gatgggatgg
    12301 ccgccgtgcg gtcgtactat ggtgcactgg tggtcgcaga aagaacgtgg cggcattgtg
    12361 tcagtgtgga actgtgcgca caacgtcggt ggtggtattc cgccgctgct gttcctgctg
    12421 gggatggcct ggttcaatga ctggcacgcg gcgctctata tgcccgcttt ctgcgccatt
    12481 ctggtggcac tgttcgcctt tgcgatgatg cgcgataccc cgcaatcctg tggcctgccg
    12541 ccgatcgaag agtacaaaaa tgattatccg gacgactata acgaaaaagc ggaacaggag
    12601 ctgacggcga agcaaatctt catgcagtac gtgctgccga acaaactgct gtggtacatc
    12661 gctatcgcca acgtgttcgt ttatctgctg cgttacggca tcctcgactg gtcaccgact
    12721 tatctgaaag aggtgaaaca cttcgcgctg gataaatcct cctgggccta cttcttctat
    12781 gagtatgcgg gcattccggg caccctgctg tgcggctgga tgtcggataa agtcttccgt
    12841 ggcaaccgtg gggccaccgg cgtcttcttt atgacactgg tgaccatcgc gactatcgtt
    12901 tactggatga acccggcagg taacccaacc gtagatatga tttgtatgat tgttatcggc
    12961 ttcctgatct acggccctgt gatgctgatc ggtctgcatg cgctggaact ggcaccgaaa
    13021 aaagcggcag gtacggcagc gggctttacc gggctgtttg gttacctggg cggttcggtg
    13081 gcggcgagcg cgattgttgg ctacaccgtg gacttcttcg gctgggatgg cggctttatg
    13141 gtaatgattg gcggcagcat tctcgcggtt atcttgttga ttgttgtgat gattggcgaa
    13201 aaacgtcgcc atcaggaatt actgcaaaaa cgcaacggag gctaatggca tgaaattgac
    13261 gctaaaaaac cttagcatgg cgatcatgat gagtggaatg atcatgggaa gcagtgcaat
    13321 ggcggcggat agcaacgaaa aaatagtcat cgcccatcgc ggtgccagtg gatatttgcc
    13381 ggagcatacg ctgccagcaa aagcgatggc gtatgcgcag ggagcggatt atctggaaca
    13441 ggatttggtg atgacgaagg acgaccatct ggttgtcctg catgaccatt atctcgatcg
    13501 tgttactgat gttgccgatc gtttcccgga tcgggcgcgc aaagacggtc gttactacgc
    13561 gatagatttc acgctcgatg aaattaagtc gctgaaattt accgaaggtt tcgatattga
    13621 aaacggtaag aaagtacaga cttatccggg gcgtttcccg atgggtaaat ctgacttccg
    13681 ggtacacact ttcgaagaag agattgaatt tgttcagggg ttaaatcact ctaccgggaa
    13741 aaatatcggt atctatccag aaattaaagc gccgtggttc catcatcagg aagggaagga
    13801 tattgcggct aaaacgctgg aagtgctgaa gaaatatggt tacagcggta aggacgacaa
    13861 agtttatttg caatgttttg atgccgatga actgaagcgt attaagaatg agctggaacc
    13921 taaaatgggc atggacctca atctggtaca gctgattgcc tataccgact ggaacgaaac
    13981 tcagcagaaa cagccggacg gaagctgggt taattacaac tacgactgga tgtttaagcc
    14041 gggggccatg aaacaggtgg cggaatatgc tgatggtatt ggtccggatt atcatatgtt
    14101 gattgaggag acatcgcagc caggtaatat caaactcact ggcatggtgc aagatgctca
    14161 gcagaacaag ctggtagtgc atccttacac cgtgcggtca gataagctgc ctgaatacac
    14221 tactgatgtg aatcagttat atgatgctct gtataacaaa gcgggtgtaa atgggctgtt
    14281 tactgatttc cctgataagg cagtaaaatt ccttaataaa gagtaatgct attgcaggat
    14341 ggcgttcatg cgccatcctc tgctaaagcg aaataatgct actcccggtt gttctattca
    14401 ttaaccggtg catcctgtta aaaaagatgc caacatattt gcgtaaaact cattcgttaa
    14461 gaaagctaac actgggcatt gttattcctt aaacagtatg aattgg
//
LOCUS       DACBVI010000114        14242 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.114, whole genome shotgun sequence.
ACCESSION   DACBVI010000114 DACBVI010000000
VERSION     DACBVI010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.114"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            47..574
                     /gene="tnpA"
                     /locus_tag="IU325_16605"
                     /pseudo
     CDS             47..574
                     /gene="tnpA"
                     /locus_tag="IU325_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311304.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            535..945
                     /locus_tag="IU325_16610"
                     /pseudo
     CDS             535..945
                     /locus_tag="IU325_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308492.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
     gene            1243..2127
                     /locus_tag="IU325_16615"
     CDS             1243..2127
                     /locus_tag="IU325_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HAP5810622.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(2304..2651)
                     /locus_tag="IU325_16620"
     CDS             complement(2304..2651)
                     /locus_tag="IU325_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HAP5810623.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(2780..3751)
                     /gene="secF"
                     /locus_tag="IU325_16625"
     CDS             complement(2780..3751)
                     /gene="secF"
                     /locus_tag="IU325_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HAP5810624.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(3762..5609)
                     /gene="secD"
                     /locus_tag="IU325_16630"
     CDS             complement(3762..5609)
                     /gene="secD"
                     /locus_tag="IU325_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HAP5810625.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(5637..5969)
                     /gene="yajC"
                     /locus_tag="IU325_16635"
     CDS             complement(5637..5969)
                     /gene="yajC"
                     /locus_tag="IU325_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP5810626.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(5992..7119)
                     /gene="tgt"
                     /locus_tag="IU325_16640"
     CDS             complement(5992..7119)
                     /gene="tgt"
                     /locus_tag="IU325_16640"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP5810627.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(7175..8245)
                     /gene="queA"
                     /locus_tag="IU325_16645"
     CDS             complement(7175..8245)
                     /gene="queA"
                     /locus_tag="IU325_16645"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP5810628.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRDKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMSAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            8464..9045
                     /gene="acpH"
                     /locus_tag="IU325_16650"
     CDS             8464..9045
                     /gene="acpH"
                     /locus_tag="IU325_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HAP5810629.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLNAHYAALETRFWQFYPRMMAQASRKAL"
     gene            complement(9066..10523)
                     /locus_tag="IU325_16655"
     CDS             complement(9066..10523)
                     /locus_tag="IU325_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810630.1"
                     /translation="MTISNSNLLKPFITGTELPPFKEDVFSKTEEQFKKSPHGKLLFI
                     SGNENLVNISAEKIDEIKNSKDFFSNIVNIIILLIKHPDKILPILHGCPVESIELIKN
                     AKRTGQWSYEMTDKNGQSIEVSYIFTDTIDNNYDGKLCLKICNPCNQNDSSTHEYKID
                     HQQLKNHIRQLEEWALLEKFYDNNAYYSKRTKQLEKKIADAGFIAGKVNADNFNVKEF
                     KDDIASMLCEQPPMRTHQISSSGYLSADKVPDLYKTNLEDAQRDLKTTPVDIDGVSLK
                     PKTREKIAEKIAKKTEEENAKKIAKQNAKTIEEKNSEKNKKETNPEDNFVLKFSPEEI
                     NEMLLDYSDEEQKKLRPFFTSDHIYCLFDAFSQGLYNVARNGSPRYAGLLRQIASKYA
                     ILQDFDQQLFWRTFSGGVFHRRISVNSEPEKTDVWRFSTQFDFPHINDNGELSRTVLM
                     FEGVAESNFKDIANHSSECFSYSCYAAPLDANAKK"
     gene            complement(10711..12528)
                     /gene="malZ"
                     /locus_tag="IU325_16660"
     CDS             complement(10711..12528)
                     /gene="malZ"
                     /locus_tag="IU325_16660"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HAP5810631.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EETSVAMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRILVAINRGEACEV
                     VLPASPFLNVVQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(12684..14057)
                     /gene="proY"
                     /locus_tag="IU325_16665"
     CDS             complement(12684..14057)
                     /gene="proY"
                     /locus_tag="IU325_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HAP5810632.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGVAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENH"
BASE COUNT         3607 a         3743 c         3354 g         3538 t
ORIGIN      
        1 tggtcttaac tggaatttaa atgcctgtaa tcgtttcaca ggtcaaatga tactttggtc
       61 tatgaaaaaa gaaaccgata tccggcgtgg aaggcattgt gttttcctga tgcatgttca
      121 cctggtcttt gtcaccagat accgacgcca gatttttgac catgacgcga cagaaaaact
      181 acgcacttac ttttcaaatg tatgtgctga ttttgaagct gaactggttg aaatggatgg
      241 cgaaccagat cacgtccatt tgttaattaa ctaacctccc aaactggcga tatccagtct
      301 ggtaaacagc ctcaaaggcg tatcgggtag gttactgcga cgagatcgac cagatattgc
      361 agtcaggtat tactacaaag gcgttttgtg gagtcctggc tattttgcca gtagctgcgg
      421 aggtgcgcca atatccgtca tccgccaata cattgaacaa cagcaaacac ctggtcaggt
      481 ggaaaaccgc gccttatatc cccgccctga aggacggggt tttacggcgc accggataaa
      541 gaaacagtca aaatcactca gcaaaacagg ctgaatgcaa aatccagcag tggggtttac
      601 cttctgcctg gagcgaaaac accagcaaga ctggaaagcc agatcggtac tttacgtatg
      661 tcgctggtga atattacgcc tgatacagat ggcaccaccc tgacgctacg tattcagggc
      721 gagtcgaatg atcccttgcc cgcattcagc ggaactgttg aatatgggca aattcaggga
      781 acaatagaca actttcagga aatcaatgtg cagaatcaat tgattaacgc ccctgccagc
      841 gtcctcgccc ccagcgatgt tgatattccg ttacagttaa agggtatttc tgtggatcag
      901 ttaggctttg tacgtatcca cgacattcaa cctgttatgc agtaaacgtt tcgcggggcg
      961 ttttgtgcgt cccgcaacat ctttccccgt cattttgtta ctctgcttac atcacccaga
     1021 ttgatagtaa aagtttgcaa caagggtgaa agtcagtaca atccccgccc gaatgtgtgt
     1081 aaacgtgaac gcaatcgatt acgtaaatga tagaactgtg aaacgaaaca tatttttgtg
     1141 agcaatgatt tttataatag gctcctctgt atacgaaata tttagaaacg caatttgcgc
     1201 ctttttcact cccgcaaggg attttcaaac agtggcatac atatgaaaaa aacattactg
     1261 gcagccggtg cggtactggc gctctcttcg tcttttactg tcaacgcagc tgaaaacgac
     1321 aaaccgcagt atctttccga ctggtggcac cagagcgtta acgttgtcgg aagctatcac
     1381 acccgtttcg gaccgcagat ccgcaacgat acctaccttg agtacgaagc attcgctaaa
     1441 aaagactggt tcgacttcta tggttatgcg gatgcgccgg tattcttcgg cggtaactcc
     1501 gatgcaaaag gtatctggaa ccacggttct ccgctgttta tggaaatcga accacgtttc
     1561 tccatcgaca agctgaccaa tactgacctt agctttggtc cgttcaaaga gtggtacttc
     1621 gcgaacaact acatttacga catgggtcgt aataaagatg gtcgccagag cacctggtac
     1681 atgggtctgg gtaccgatat cgatactggc ctgccgatga gcctgtccat gaacgtctat
     1741 gcgaaatacc agtggcagaa ctatggcgca gcgaatgaaa acgagtggga cggttaccgt
     1801 ttcaaaatta aatactttgt gccgattacc gatctgtggg gcggtcagct gagctacatc
     1861 ggctttacca acttcgactg gggttccgat ttaggggatg acagcggtaa tgcaatcaac
     1921 ggtattaaga cccgtaccaa taactccatc gcttccagcc atattctggc tctgaactac
     1981 gatcactggc actactctgt cgtagctcgt tactggcacg acggtggtca gtggaacgac
     2041 gatgcagaac tgaacttcgg caacggcaac ttcaacgttc gctctaccgg ctggggtggt
     2101 tacctggtag taggttacaa cttctgatga atgaaatgcc ggatcttgta tccggcattt
     2161 cagtttgtta acaacgtgcg ctttgttcat gccggatgcg gcgtaaacgc cttatccggc
     2221 ctacaaaagc acgtaaattc aatacattgc tgagattacg ttggcctgat aagcgtagcg
     2281 catcaggcaa ttttgcgttt ttatcacttc ttcttgttca tcatcgcttt caggtcagcg
     2341 aatgggttgt acttcgcttc accgacatct ttctgcgcat cttcccctgc gataacggtc
     2401 gtaccatact gatctgcttc ggtatatttt gaatgctcgt gatcgtggca ataaagacac
     2461 aataactccc agttactgcc gtcttccggg ttattggtat ggtcgtgatc aatgtggtga
     2521 acggtaagtt cacgcaggtt ggaataaaca aactcgcgag aacagcgacc gcagacccac
     2581 ggatagattt ttaatgcttt ttcgcgatag ccgctttcta accgcgcata gttttttggg
     2641 attatagcca tgaaaagtta cctggatgaa atgtagggtt acgcgcagtt tcccaagatg
     2701 cggggatttt cgcaatctcc atacatcaaa aaaaatcccg atcttctgac cgggattttc
     2761 aacatcaacg ggaacttgat tacggcagaa ttgacggctg atccgcccct tctttttcca
     2821 ctttctgctg cagcatgtgt tcgcgcttca tacccagttt cagagccagc gccgatgcta
     2881 cgtagatgga agatgcagta ccgatggaaa caccaatcag catggtcagc gagaagcctt
     2941 ccagtaccgg accaccgaag aggtacagca tcaggataac catcaaggta gtaccggatg
     3001 tgatcaaggt acggtgcagc gtctgggtca aggacacgtt aaagatttcg taaggcgtac
     3061 cgcgacggat cttgcggaag ttttcacgaa tacggtccga taccacgata ctgtcgttaa
     3121 gcgagtaacc gataactgac atcaacgatg ccacaatggt caggtcaatc tcgatatgga
     3181 ataacgacaa aatacccagc gtaatgataa cgtcgtgcgc cagcgcaata acaacccctg
     3241 ccgccagtcg ccactcaaag cggaaaccta cgtacacgag gatagacagc agcgccgcca
     3301 tcaacgccat cgcaccggtt tgcgcaaggt ctgcccccac gctcggaccg acgaactcaa
     3361 tacgcttcac tgccgcattc tgattggtgg attcgttaat caccttcagg acctggctgc
     3421 ccagcacctg accgccggtt tcgccttcag caggcggcat acggaccatg atgtcgtggc
     3481 tgctaccaaa gttttgcagc atcggctctt caaaaccggc tttttgcaat gcatcacgca
     3541 ttacgtcaat ttcagccggt ttttcgagcg taatttcgat aaccgtacca ccggtgaaat
     3601 ccagccccca gttaaagccg cgcacgccca taataacgat agcggcgatt aacagcagac
     3661 cggagatgcc aaaagcccag tagtcccagc gcataaagtc atagacttta cggccgtggt
     3721 ttagttgttc aacagtatat tcctgtgcca catcgcactc ctcagattga cagcttcttg
     3781 acgcgcttgc cgccatatag caggtttacg atggcacgcg taccgacaat cgcggtaaac
     3841 atcgacgtcg ccacaccgat accggtagta atcgcgaacc ctttaattgc cccggtaccc
     3901 actgcgtaca ggatgatgac tttaatcagc gtggtgatgt tcgcatcgaa gatagaactg
     3961 aatgcgccac gataaccttc atcaattgcc tgttgaacag tacgtccgtt gctcaactct
     4021 tctttaatac gttcgttgat aagtacgttc gcatcgaccg ctaccgcaag agttaagacg
     4081 atacccgcga tacctggcat actcagcgtt gcgcctggca acagcgacat aatgccgaca
     4141 atcaagatca agttggcaat cagagcactg gtcgcaatca gaccaaactt cttatagaag
     4201 atgatcatga acagaataga aaccagcaga ccggcaaggc aagcctccag cccctgctca
     4261 atgttctgca tacccagggt cgggccaatt gtacgttctt caacaatctg aatcggcgcg
     4321 atcaacgcac cggcacgcag cagcagtgac agctgacggg cttcgttcgg gttgttgatg
     4381 ccggtgatac ggaagctgtt acccagacga gactggatgt tggcgatgtt aatcacctct
     4441 tcctgtttca ccagaaccgc acgaccgttt gcatctttct taccgctgtc tttgtactcc
     4501 acaaacaggg tcgccatcgg tttgccgatg ttgtccttgg tgaagttaga cataatgttg
     4561 ccacctgcgc tgtcgagcga gatgttaacc tgcggctggt tgtattcgtc ctggctggaa
     4621 gtggagtcgg tgatatggtc accggtcaga attacgcgtt tgtacagcac aactggctga
     4681 ccttcgcggg tctgtttcac ttcagagtcg ccaggtacgc gaccggatgc cgctgcggcc
     4741 tgatcaacgt tagtgtttac cagacggaat tccagcgttg ccgtcgcacc cagaatctct
     4801 ttcgcacgcg cggtgtcctg aatacctggc agttcaacaa cgatacggtc agcaccctga
     4861 cgctgaacca ccggctccgc cacaccaagt tggtttacac ggttacgcag gatattaatg
     4921 ttctgctgca ccgcatattc acgcgcttca ctaaggcgag catcgctcat taccgcacgc
     4981 agctggttgc tgccctggct actaatcacc aggtccggat gacgcttgct cagatacgca
     5041 atggcttcat cacgagcttt agcatcgcgg aaagtgatgc tcaggccgta gttgttttct
     5101 ttacgaacgg tggtatacgg gatgcccttt tcgcgcaggt cgctgcgcag gctatcgata
     5161 ttttgttcct ggagtttgcc aagcgcggtg tccatatcca cttccatcag gaagtgaacg
     5221 ccgccacgca ggtcaaggcc gagcttcatc ggctcagcgt gaatagctgc cagccagcgc
     5281 ggcgttgccg gggcaaggtt aagcgccacg acgtatttgt cacccataac gcccattaat
     5341 gcttcacgag cgcgcagctg ggtgtcagtg gagtcgaagc gcgcaagaat agcgccctct
     5401 tccagtgcca cagacttagc agttattttt tcttcttgta acgttttctg gacctggatc
     5461 agcgtttgct cactggcggc gactccgcgc gcaccagtga tctgaacagc cggatcctca
     5521 ccaaacaggt tgggaagcgc atacagcaga ccgatgacaa tcaccacgat cagcatgacg
     5581 tacttccaca aaggataacg gtttaacacg gcaattccct tagggaaaaa ttttaattac
     5641 agcgccttca tggtgccttt cggcaggacg gcagctacga agtcacgttt aataactact
     5701 tcagtggtgt cattcagcgc gatagcaatg tagccgtttt ccgctacttt ggttacgcga
     5761 ccaaccaggc caccgttcgt cagaacttca tcacctttgg caatggagtc catcagcttt
     5821 ttgtgttctt tggtgcgctt ctgctgtgga cgcaggatca tgaaatagaa aatcagaccg
     5881 aataccacca gcatcaaaat caaagacatc gggctacctt gcgccggtgc acccgttgcc
     5941 gctaccgcat cagaaataaa aaagctcatt aaatttccct cattattaat attaatcaac
     6001 gttcaaaggt ggtacttctc gcccctgacg ctggtaaaaa tcagttacga agctctctaa
     6061 tttaccctct tcaatagcct tgcgtaaacc cgccatcaaa cgctggtagt aacgaaggtt
     6121 atgaatggtg ttgagtcgcg cgcctaatat ttcgttgcaa cggtcgagat gatgcaagta
     6181 agcgcgtgaa taattgcgac aggtgtagca atcacactca ggatcgagtg ggccagtatc
     6241 gctcttatac ttcgcattgc ggattttcac cacgccatcg gtcacgaaca aatgaccatt
     6301 tcgggcgttg cgggttggca ttacgcagtc aaacatatcg ataccgcgac gcacaccttc
     6361 aaccaggtct tctggtttac caacgcccat caggtaacgc ggtttgtctg ccggaatttg
     6421 cgggcataca tgctccagaa tgcggtgcat atctgctttc ggctcaccca cagccagacc
     6481 gccgacagcg tagccatcaa aaccgatatc taccagacct ttaacagaaa tatcacgtaa
     6541 atcttcgtaa acgctgccct gaatgatacc aaacagcgca tttttgtttc cgagactgtc
     6601 aaaacgctca cggctacgct tcgcccaacg cagagacatc tccatggagc gttttgcgta
     6661 atcccagtca gcaggatacg gcgtacactc atcaaagatc atgacgatat ccgaaccaag
     6721 atcgtactga atctccatcg acttttccgg gtcgaggaaa atcgggtcgc cgttgatcgg
     6781 gttacggaag tgcacgccct gttcggtgat tttacgaata tcaccaaggc tgaagacctg
     6841 gaagccgccg gagtcggtaa ggattggtcc tttccactgc ataaaatcgt gcagatcgcc
     6901 gtgcagtttc atgatttcct ggcccgggcg cagccacagg tggaaggtgt tgccgaggat
     6961 aatttgcgcg ccagtggctt caacttcttc cggcgtcatc ccttttacgg tgccgtaggt
     7021 gccaacaggc ataaaacaag gcgtttccac tacgccacga tcaaagacca ggcggccacg
     7081 gcgtgcgcga ccgtcggtgg tgtccagctc aaatttcatt ttttctccac tacgtcagaa
     7141 aaacagtcca acgttttaaa ccagcgccgc ggaattactc cccgacgcgc tcattaattg
     7201 cctgcggatt gtacgtgata aacatcgcat caccgtaact aaaaaagcga tatttctctt
     7261 ctaccgctgc tttataggcg ctcatggtgt gttgataacc ggcaaaggcc gaaaccaaca
     7321 taatcagcgt cgactctggc aagtggaagt tcgtcaccag cgcatcgacc actttgtact
     7381 gaaagcccgg atagataaag atttgggtgt cgtcaaagaa cggttcgatg agatcgtttt
     7441 tcgccgcctg agccgcgctt tccagtgaac gtaccgaggt ggtgccaacc gcaatcaccc
     7501 ggttaccgcg cgctttcgcc gccagtaccg cgtctaccac atcctgcggt acttcagcgt
     7561 attccgagtg catgatgtga tcttcaatgg tgtcgacgcg caccggctgg aaggtgcccg
     7621 caccaacgtg cagggtgacg aacgccatct ccacgccttt gtcgcgtaat ttttccagca
     7681 aaggttcgtc aaaatgcaga ccagcggtcg gggctgcaac cgcgcccggt ttttcgctat
     7741 aaacggtttg ataaagttcg cggtcagcgt cttcgtccgg acggtcgata tacggcggca
     7801 gcggcatatg gccgatgctg ttgaggatat ccagtaccga acgatcatca ttaaattcaa
     7861 cttcaaacag tgcgccgtgg cgcgcggtca ttgttgcgtt gatactttcg tcatcgccca
     7921 gtaacagttc tgcgccaggt tttggcgctt tcgaggcgcg aatatgcgca agaatgcgtt
     7981 tgtcgtcgag catccgctca accagcactt caatcttgcc gccgctggct ttacgcccaa
     8041 acaggcgcgc cgggatcacg cgggtattat taaaaaccag aagatcgccg gggttgagct
     8101 tatcgagtaa atcggtgaaa gtaccgtgcg tcagcgcgcc cgtcggcccg tccagcgaca
     8161 gtaaacgaca gctactgcgt tcaggcatgg gatagtgggc aatcagggat tcgggcaatt
     8221 caaaggagaa atcggtaacg cgcatgacac tgactcagac taaaataaga ggcgggtagt
     8281 ctagtgccgg ggcgctctcc ctgcaacctt taccctcctt gatggatgac ataatctata
     8341 cacaaaatca ttcaagatgc atcgagatgg caactgaatg agtccccagg agcttacatt
     8401 aagtaagtga ctggggtgag tgaaggcagc caacgaagag gcagtttgaa ggatgaagtg
     8461 tatatgaatt ttttagctca cctgcatcta gctcacctgg cggaaagctc gctttctggc
     8521 aatttactgg ctgatttcgt acgcggaaat cctgaagaaa gttttccgcc cgacgtcgtg
     8581 gctggcattc atatgcatcg gcgcattgat gtactaaccg acaatctgcc ggaagtccgc
     8641 gaagcacggg aatggtttcg tagtgaaacc cgccgcgttg cgcctattac cctggatgtc
     8701 atgtgggatc actttctttc ccgccactgg tcgcagctgt cgccggactt tccgctacag
     8761 gaatttgtct gttatgcccg cgaacaagtg atgacgattt tgccggactc accgccgcgc
     8821 tttatcaatc tgaacaacta cttatggtca gagcagtggc tggtgcgcta tcgcgatatg
     8881 gatttcatcc agaacgtgtt aaacggcatg gcaagccgtc gcccacgtct ggatgccctg
     8941 cgtgactcct ggtacgattt aaacgcccat tatgccgccc tcgaaacccg cttctggcag
     9001 ttttatccgc ggatgatggc gcaggcgtca cgcaaggcgc tctgaaactt caagctccgg
     9061 cgtcactatt ttttagcatt cgcatccagc ggggcggcat aacaactgta agaaaaacat
     9121 tcgctggaat gattggcgat atctttaaag ttgctttcag caaccccctc gaacatgagt
     9181 accgttctgg acaactctcc gttgtcattt atatgtggaa aatcaaattg tgtactgaat
     9241 cgccatacat ctgtcttttc tggctcgctg ttcacagaga tacgtcgatg gaagactcct
     9301 cctgaaaacg tgcgccagaa caattgttga tcaaaatctt gtaatatcgc atacttggat
     9361 gctatttgac gtagtaaacc tgcataccgt ggactgccat tacgagcaac attatacaaa
     9421 ccctgactaa aagcatcaaa caaacaatag atatgatcac tagtgaaaaa aggtcttaat
     9481 tttttttgtt cttcatcgct gtaatccagg agcatctcat tgatttcttc cggtgagaat
     9541 tttaagacaa aattatcctc aggatttgtt tctttcttgt ttttttcact attcttctcc
     9601 tcaatggtct tcgcattttg tttcgcaatt tttttcgcat tttcttcctc agtttttttc
     9661 gcaatttttt ccgcaatttt ttcccgagtt tttggtttta gactaacacc atcgatgtca
     9721 accggtgttg ttttcagatc tctctgcgcg tcttccaaat ttgtcttata gagatcgggt
     9781 actttatcgg cagataagta tccggatgaa gatatctggt gtgttcgcat ggggggctgt
     9841 tcacaaagca tcgaggctat atcgtcttta aactctttta cattaaagtt atcggcatta
     9901 accttccctg ctataaaccc cgcatcggca attttcttct ctaattgttt tgtacgttta
     9961 gaataataag cgttgttatc ataaaacttt tcaagaaggg cccactcctc aagttgcctt
    10021 atgtgatttt tcaactgttg atgatctatt ttatattcat gtgtactaga atcattttgg
    10081 ttgcaggggt tacagatttt taagcataat tttccatcat aattattatc tatggtatca
    10141 gtgaaaatat aggacacttc aatactctgc ccgttcttat cagtcatctc atatgaccat
    10201 tgccccgttc gtttcgcatt cttaatgagt tcgatggact ctacaggaca accatgaaga
    10261 atgggcagaa ttttatcagg atgcttaatg agtaaaatta ttatatttac aatatttgaa
    10321 aagaaatctt tgctattttt tatttcatcg attttttccg ctgaaatgtt tactaagttt
    10381 tcatttccgg aaatgaaaag caatttgcca tgggggcttt ttttaaattg ttcttctgtt
    10441 ttagaaaaaa catcctcttt aaaaggtggc aactcagtac cagtaataaa cggttttaat
    10501 aaattcgaat ttgaaattgt catatctttg ctccactaca gggttatcct ggagcatgtt
    10561 gtacataaca tcatgggcaa agaataccaa aaatcgctca tatcgatcgg gacaaatatt
    10621 taacaaattc agccctgaca ttgagcagct tttttgcgct gcgtagtgtc agggctggtg
    10681 actaacgacg taacaaataa aacagatgct ttagttcatc cacaccgtag ccgaaatggc
    10741 aggcaaagcc agaatcccgt ctgtcagttg cccatggcct tctttgcgtt gccattgcac
    10801 gacattaaga aacggtgacg cgggtagcac cacttcacag gcctcgccac ggttgattgc
    10861 caccagtata cgctgctgat tcagcacgcg gacaaatacc accacgttat cttccgcata
    10921 cagcacctga cagccgccac gacgcagcgc ctgacttttc ttacgcagcg caatcattcg
    10981 ctggtacaac gcgaataacg ccgtatcctg cttttccacc tgccagggga acggcttacg
    11041 gcaaaacgga tcgtttttac catccagtcc cacttcatca ccgtaataaa tgcacggtac
    11101 accaggccag gtgaacaacc agaccaccgc cagcggcagg cgcgcaatat cccgaccgag
    11161 cagcgtttta aatcgcgcag tatcgtggct atcgagctga ttaaacatac gcagttgctg
    11221 ttgatgagaa agtccggcgc ggtaattatc catccaggcc atacaggttt gggcgtcaat
    11281 ttgctgcgga tcataagaga tgtcggtatt ggcaagaaat ccccacaacg ggaatgtgaa
    11341 gccgcgatag ttcatggcgg catcttccac atcggcctgt aaccattgcc gtgcatcgcc
    11401 aaaatgctct ccaactatat acgcttccgg ctgggtttct ttcgccgctt cggtgatccc
    11461 ggctacgtgc tgcaaattat tgcgcgcccc acccgcttcc cccagcatat gcaccacatc
    11521 cagccgccag ccgtccatat tccacggcgc tttcagccag tggcggacaa tactgtcttc
    11581 cccgcgataa atttcattca ccagactttc cgactgataa tccagcttcg gcaagctggc
    11641 atagcccagc cagtcgagcg ccgtgccatc gtcactaaac gagtaccagt cgcgccaggg
    11701 cgattcgggg ttgtgacaag caccacccgt cccgcgattg tgtctgtcaa accaggcatg
    11761 ggaatcgcca ctgtggttaa acacgccgtc cagcaccagc cgcattccca gctgctgcgt
    11821 attgtgtcgc aaacgcaaca acgccccatc accgccaaac tgcggatcga catggcgata
    11881 atcctcggta tcgtatttat gtacgctggg agctttaaac accggattga gatacagcgc
    11941 cgtcacgccg agctttttca gatacggcag tttttcgctg ataccgtcca gatcgccgcc
    12001 atagaacgtt gatccgcccg cctgcgccgt gaccggttca tcccagtcac gcaatatgat
    12061 ctcttgtccg gctgcgtgat ggtaataaac atgatcctgt tcagcttcac gaggaagact
    12121 acgggcgaaa cgatcaggga agatctgata aaaaatctga tccgcagccc attgtgggcc
    12181 gatatccggg acatcgacgg caaactgctc cagtcgtgct ggcggcattc ggctgaagcc
    12241 ctgcggtgta aaccaacgct ggcgatcgtg ccacagcaat ttgaaactat aacgccgccg
    12301 gggttgcccg ctggagagat cgatcgccgc ccgccatgcg gtaacgccag gctgcggctg
    12361 actgcgctgc ttatgcatcg ctactgacgt ttcttcgttg tcgtgttctg tacgcagcat
    12421 aatgcgctgc ggtgggtctt cgcccgtcag ccacagcgta atgagcagtt gatctttgct
    12481 ttgtttaaca aatgggggca ccggcaggtg ccatgcattt aacatcataa ttcccctgtg
    12541 atgaaaatct gatcaccttg ccatagccta atgcaggatc actcatcatc tgaaggatat
    12601 ttgggggagg aggacggagg cggataactt tcggaatagc cggatcagga tttcctcctg
    12661 aaccggctta cagaacgcaa ggattagtgg ttttcagcca gctgacgatc atggcggcgc
    12721 ttaaacatcc agccaatcaa cagcacaaca atccacgcga aaccgacata cagcgagata
    12781 cgcgtatcag ggtgataacc aatcaacccg ataataaaga gcaggaaaat caaaccgccg
    12841 atggtcgttg ctaccccacc cggtacttta aatttcagcg ccttaacttc ttctggcggt
    12901 aaacggcgac ggaaggcgat ttgcgacagc aggatcataa tccacaccca caccgtggcg
    12961 aaggtcgcca gcgaagcgat caccaggaag acgttttccg gcatgatgta gttcagatat
    13021 accgcaaaca atagcgcggt agtcatcacc agcaccgtga cccacggaat accacgacgc
    13081 gacgttttgc tgaaaatttt tggcgcgctg ccctgctctg ccataccatg gagcatacgg
    13141 cctacgccaa atacgtcact gttaattgcc gaaagcgaag cggtcagcac aacaaagtta
    13201 aggatgctgg cggcaaaggt aatccccata tgctggaacg tcagtacgaa cgggctaccg
    13261 gcagtgccaa cctgattcca cgggtagata gacataatga cgaacagcgt accgacgtag
    13321 aacaccagaa tacgcatcgg cacggagtta atcgcgcgcg gaatagattt ctcaggatct
    13381 ttcgcttcac cggcggtaat cccgataatt tcgatcccac cgtaagcaaa catcaccatt
    13441 tgcaacgaca tcaccatacc aagccagccg ttactgaaga agccgccgtt gctccacagg
    13501 ttatgaatac cggtcggttg cccgccgttg ccaatccccc agatgatgat gccgaaaccg
    13561 gcgacaatca tgatgatgat ggtggcgact ttaaagaacg agaaccagaa ctccagctca
    13621 ccgaatacct tcacgctcat caggtttacg gcgcaaatga tcagcaccac gctcagtacc
    13681 caaatccagt gcggcaccgt cgggaaccag acacccatat agataccaaa agcggtcaca
    13741 tcggcgatgg cgacaataag gatttcaaag cagtaggtcc agccagtaat gtaaccagcc
    13801 agcgggccga ggttttcctg cgcataacgc gagaaagagc tggcggccgg gttatgtacc
    13861 gacatttccc ccagcgcgcg cataatgata tacgccgcga caccaccgat aatataggcc
    13921 aacaacacgc tcggaccggc cattttgatg gcgtctgccg aaccgtaaaa caacccggtg
    13981 ccaattgctg aacccagtgc cataaagcgt atgtggcggg tacttagccc acgctttagc
    14041 ttgttcttac tttccatcaa ttcctaaccc atcaacacaa taaataaata tcctccggca
    14101 tagccggagg tttttcaaat gcgcctataa ggctctctta ccagccgcgc cctaacaggc
    14161 gcacacgatc tgacatttgc atccaacttc gttacttacg gcccgtaaac gggctgcccg
    14221 gatagggaat cgataactgc tc
//
LOCUS       DACBVI010000115        14220 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.115, whole genome shotgun sequence.
ACCESSION   DACBVI010000115 DACBVI010000000
VERSION     DACBVI010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.115"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            6..281
                     /locus_tag="IU325_16670"
     CDS             6..281
                     /locus_tag="IU325_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HAP5810633.1"
                     /translation="MIFYGIIILHDVPYLIAKKRNHPHADAIHVAGWVSLFTLHVIWP
                     FLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIKSSSAE"
     gene            287..1423
                     /locus_tag="IU325_16675"
     CDS             287..1423
                     /locus_tag="IU325_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HAP5810634.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSAAAEQKQILSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1420..2394)
                     /locus_tag="IU325_16680"
     CDS             complement(1420..2394)
                     /locus_tag="IU325_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP5810635.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTVLLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAQPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQTAESED"
     gene            2518..3258
                     /locus_tag="IU325_16685"
     CDS             2518..3258
                     /locus_tag="IU325_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HAP5810636.1"
                     /translation="MLINRNIVALFALPFKASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDTFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMVDRNESFTFVTGVSWRF"
     gene            complement(3388..5358)
                     /locus_tag="IU325_16690"
     CDS             complement(3388..5358)
                     /locus_tag="IU325_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HAP5810637.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQDGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLADHIDSVFGPGESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPIAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDESKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPADAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     PQILTFIPWFSWANRGEGEMRIWVNEEKHCHP"
     gene            complement(5369..6769)
                     /locus_tag="IU325_16695"
     CDS             complement(5369..6769)
                     /locus_tag="IU325_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5810638.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGITAMETLLPALFHLLLAMAIRFCKLNNP
                     MMSRIASDLRQRHVQP"
     gene            6995..7810
                     /locus_tag="IU325_16700"
     CDS             6995..7810
                     /locus_tag="IU325_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP5810639.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     AIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDSDPRWLTHLS
                     VPQQTKVADPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADAAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            complement(7842..8537)
                     /gene="araD"
                     /locus_tag="IU325_16705"
     CDS             complement(7842..8537)
                     /gene="araD"
                     /locus_tag="IU325_16705"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP5810640.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRCYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRLMTAEEINGEYEYQTGEVIIETLEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(8531..9391)
                     /locus_tag="IU325_16710"
     CDS             complement(8531..9391)
                     /locus_tag="IU325_16710"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP5810641.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRASLVTAMIETGVAIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTQQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTRPVTG
                     QSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            complement(9384..10046)
                     /gene="ulaD"
                     /locus_tag="IU325_16715"
     CDS             complement(9384..10046)
                     /gene="ulaD"
                     /locus_tag="IU325_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP5810642.1"
                     /translation="MSRPLLQLALDHSSLEDAQRDVMLLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPNKIIVADWKVADAGETLAQQAFTAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPVQVAGDFHAQIDAIWGGA
                     RA"
     gene            complement(10043..11539)
                     /gene="lyxK"
                     /locus_tag="IU325_16720"
     CDS             complement(10043..11539)
                     /gene="lyxK"
                     /locus_tag="IU325_16720"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HAP5810643.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRPLLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAALTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTMRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGFHARIKEHTL"
     gene            complement(11543..12529)
                     /gene="yiaO"
                     /locus_tag="IU325_16725"
     CDS             complement(11543..12529)
                     /gene="yiaO"
                     /locus_tag="IU325_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HAP5810644.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSMAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLTPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGNQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            complement(12542..13819)
                     /gene="yiaN"
                     /locus_tag="IU325_16730"
     CDS             complement(12542..13819)
                     /gene="yiaN"
                     /locus_tag="IU325_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HAP5810645.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            complement(13822..>14220)
                     /gene="yiaM"
                     /locus_tag="IU325_16735"
     CDS             complement(13822..>14220)
                     /gene="yiaM"
                     /locus_tag="IU325_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HAP5810646.1"
                     /translation="LRYGFQTSILSVDELSRYLFVWLTFIGAIVAFMDNAHVQVTFLV
                     EKLSPAWQRRVALVTHSLILFICGALAWGATLKTIQDWSDYSPILGLPIGLMYAACLP
                     TSLVIAFFELRHLYQLITRSNSLTSPPQGA"
BASE COUNT         3428 a         3706 c         3767 g         3319 t
ORIGIN      
        1 ttctggtgat attctatggg attattattt tacatgatgt tccctacctg attgctaaaa
       61 aacgtaatca tccacatgcc gacgccattc atgttgctgg ttgggtaagt ctttttacgt
      121 tacatgttat ctggccattt ttgtggattt gggccacgct ttaccgcccg gagcggggat
      181 ggggaatgca aaaccatgac tcctccgttg tgcaactaca acagcgtatt gccgggctgg
      241 agaaacagct cgccgacatc aaatcctctt ctgccgagta atacttatgg atttactgat
      301 tattttaacc tatgtggctt tcgcatgggc aatgtttaag atcttcaaaa ttcctgtaaa
      361 taaatggacc attcccacag cggccctggg tggcatattt attgtcagcg gtttaattct
      421 gttaatgaac tataaccatc cgtatacctt taaagcgcaa aaagcggtta tttctattcc
      481 tgttgtccca caggtgacag gcgtggtgat cgaagtgacg gacaagaaaa atacgctgat
      541 taaaaaaggt gaggtgctat ttcgactgga cccgacgcgt tatcaggcgc gggtggatcg
      601 gctgatggcg gatatcgtta ccgcagaaca taaacagcgg gcgttgggcg cagagttaga
      661 tgagatggcg gcgaatactc agcaggcaaa ggccacgcgg gataaattcg ctaaagagta
      721 tcagcgttac gcacgcggca gtcaggcgaa agtaaacccg ttttcagaac gtgatatcga
      781 tgtggcgcgg caaaattatc tggcacagga agcctccgta aaatcagcgg cggcggaaca
      841 aaaacagatc ctgagccagc tggatagcct ggtgttgggt gaacattctc aaatcgccag
      901 cctgaaagca cagctcgcgg aagcaaaata taaccttgag cagacgatag tgcgtgcgcc
      961 gagcgatggt tatgtgaccc aggtactgat tcgtccgggt acctatgccg cgtcgctgcc
     1021 gctacgtccg gtgatggtgt ttatacccga tcagaaacga caaatcgtgg cgcagttccg
     1081 tcagaactcc ttgctgcgac tggctcctgg cgacgatgcg gaagtggtgt ttaatgctct
     1141 gccaggtaag gtattcagcg gtaagctggc agccattagt ccagccgttc ccggcggagc
     1201 ttatcagtcg accggcacct tacagacgtt aaacacagcg ccgggttcag atggcgttat
     1261 cgcgaccatt gaactggatg agcatactga tttgagcgca ttaccagacg gtatttacgc
     1321 ccaggtggcg gtctactctg atcatttcag ccatgtctcg gtgatgcgca aagtactgtt
     1381 acgcatgacc agctgggtgc attaccttta tctcgatcat taatcttcgc tttccgccgt
     1441 ttgttcaggc cgcagttgct gcgactgagc aagctggtgg cgaatttcac gcagcatttc
     1501 atccagcaca tactggaccc gccagggctg gtattcccgt ttacggaaga tagccaccag
     1561 atccagccac cattcatacg ggtgctgaaa gcagggcacc agcgtgccgt cttgcaggtc
     1621 gctctgcaca cttttgtctg gcgcaaacac aatccccagg tgatttcgtg ccagctccag
     1681 cgcagattgc gtgttgtcgc aaacgtaatt tcccttcacg cgataatccc gcacctcttt
     1741 actcccggcg acgttgaagc gccagatatt cgcgtcgtcg atcatcatcg agtcgatcaa
     1801 aatacaggag tgatgttcga gttcgtcagg acggctaatg ggatgtttct caagatagcg
     1861 ctggctggca taagcggtta cggcatattg ggtaataaaa ctggcaacca gcgattcatc
     1921 ttttggttgg gcgtagctga ttaaaacatc acaatcatcg ggaaattcga cgccttcata
     1981 aaaggcatta cgctcaagat tgcaggtttt tagcgataag gtgatatcac cgatatcttt
     2041 aattttatcg attacatgtt tggataaata ggtaattatg ccggttggtg catagatagt
     2101 gacccgacca cgtttctcat gcttataatc tgcaataaaa ttattaagct gctcgtttct
     2161 gtccagcatg tcgttgatgt acggtaacag cacggtgcca aaaggtgtga gcatcagctg
     2221 tcgggtggtt cggtcaaaga cttttaaacc cacttttgat tcaaaatcag caagatattt
     2281 gctgacgttg gcctgtgcga tgccaagtac agtcgcggca tggctgatat tttcactggc
     2341 agcgattacc gagataattt ttaactcccg gtacttaagt tgtaattttg tcatcgcaac
     2401 agcccaatta tatataattt tatatattac tatataaata tgaatcttgc accggataaa
     2461 ttgtaagcat tactatgccg actctttttt atcttatttt aaaatggaat accggacatg
     2521 ttaattaacc gcaatattgt ggcgttattt gcgttgcctt ttaaggcaag cgcaactgct
     2581 tctgaattat ccattggtgc tggtgcggct tataatgaat cgccttatcg cggttataat
     2641 gaaaatacaa aggcaattcc gctgattagt tatgaaggtg atacttttta tgttcgtcag
     2701 accacgttag gttttattct gtcgcaaagt gaaaaaaatg aacttagcct gaccgcatcc
     2761 tggatgccgc tggaatttga ccctgccgat aatgacgatt atgccatgca acagcttgat
     2821 aagcgtgata gtacggctat ggcgggggtt gcctggtatc accacgagcg ttggggaacc
     2881 gtgaaagcct ctgcggctgc ggacgttctg gataacagca acggctgggt gggggagcta
     2941 tcggtattcc acaaaatgca gataggtcgc ctgtcgctga cacctgcgct gggcgttctc
     3001 tattatgacg agaatttcag tgactattac tatggcattt cagagagtga gtcccgtcgt
     3061 agcggtctgg caagttattc cgcgcaggat gcctgggtgc cctatgtcag cctgacggca
     3121 aaatacccga taggagagca cgtcgtattg atggcgagcg caggatacag cgagctgccg
     3181 gaagagatta ccgacagccc gatggttgat cgtaatgaga gttttacctt tgtcactggc
     3241 gtgagctggc gtttttaatt caccggtgga tgtcggtgcg gctggcctac gctctcgcac
     3301 cgacttgtag gcctgataag acgtgttaag cgtcgcatca ggcaatgtgc accctacggt
     3361 ggatgcggcg cgagtgcctt atccaaccta cggatggcaa tgcttttcct cattcaccca
     3421 gatccgcatt tcgccttcac cccggttggc ccagctaaac cacgggataa acgtcagaat
     3481 ttgcggctgg cgtttggctg gcgcgctgtc gtaatgccac agcggttgct gctctggatt
     3541 gctctgttca taccggtaac ccggtgcctg gattaagatc ttatggctga acaatccctt
     3601 accttcaaat gtcgtaaatg gcgcatcggc gggcagccac aggttatgca gtgactcgcc
     3661 gttgtcggcc tgttccaggc aatacaccag cgggccgcgc tgaatcgcca cttttccggc
     3721 gacgtgacgc accagagggt taccgtaaac gcggcgtacc ggcatcggca aagtcagatt
     3781 cagcgtatcg ccctcctgcc attcgcgggt aatgtgcaaa taccctttac gaatatcctg
     3841 ctcgacctct tccccattca atatgatctg cggctgtgtg caccagtccg gcagacgtaa
     3901 agccagcgta tgacgcaccg gctggggcga ttcaaccgca attgtcacct gttcctgcca
     3961 cggatagttc ccgctaaccc gcaggcgcaa cgtgccattt tctaccggca cttccatgct
     4021 gtttcctgcg tagatgttga tatacaacgc atcttcacgc ggtgtgtaga gataatgacc
     4081 aatcgaggtc agcacgcggg cgatatttgg cggacaacaa gcgcagccaa accaacgctg
     4141 gcggatcggt ttaacgtgat cgtagatatg gttgaatttc agcgattttg gatgtacttc
     4201 cagcggattc acatagaaga aatgtttgcc atccagtgcc atgccgccga gcacagtgtt
     4261 gtacagtgcg cgctccatca catcggcata ttgactgtcg ccttccattt ccagcattcg
     4321 tcgggcgaac atcatcaggc cgatggaagc acaactttcg gcgtaaaccg tgtcattcgg
     4381 cagatcgtaa tcgctgctaa acgcttcgcc gctgctttgt gagccaatgc cgccggtaat
     4441 atataactga cgctgggcca tattgttcca cagccgcagg caatcctgac gcttgctttc
     4501 atcgtgactt aaacgcgcca gatgtgcgac gccagtcatc aggtagacaa agcgtactgc
     4561 atgtccgatg gcggtttgtt gctgtgcgat gggcaaatgc gcctggctgt aggctttatc
     4621 tttcaccatc cacgccgggc cgtaggtgtg ccagtgcgag gtctgcccgc gcttttcata
     4681 ttcctggtcg taatagtgcg gttgcgcacc acgctgttcg acaaaatagt tcgtcagcgc
     4741 caggtagcgc ggctcttcgg tcacttcata caggcgcatt agtgccagtt caatttccgg
     4801 gtgaccagga taaccgtgta acttactttc acctggacca aatacgctgt cgatatgatc
     4861 ggccagacgg caaaccactt ccagcaagcg ccgcttgccc gtggcctgga agaaggcgac
     4921 tccggcttca atcagatgac cggcgcagta aagttcatga cactccgcca gattgctcca
     4981 gcgttcttcg ggcgctttta ccgtaaagta agtattgaga tagccatctt cgcactgggc
     5041 ggaggcgatc agttcaatca cctcgtcagc agttttttcc agttcggcat ccggcttctg
     5101 gcacagcgac caggctaccg cttccagcca tttggcgacg tcgctgtcct gaaacaccat
     5161 cccgtaaaat tcaccgtctt gcagtcctgc ggcaatgcga aagttttcaa tcgcatggct
     5221 gggttccgct tctgggatac gatcgttcaa ggcgtcccac tgataaggaa tcaccacatc
     5281 gcggaccagt tgttggtact gaccgaggaa cggatcgctg accgtcagtt tatgcagatc
     5341 gacttccgaa atgttcatct tgtgctcctt aaggctgtac atgacgctga cgcaggtcgc
     5401 tagcaatgcg tgacatcata gggttattga gtttgcaaaa gcggatcgcc atcgccagca
     5461 gcaggtggaa tagcgcaggc agcaatgttt ccattgctgt gatcccgcca agcgaactgg
     5521 cggtctggtt ttccacgcct ggctggtagg cgacaaaaat aaacagcagg ctgatgatcc
     5581 cggcgctgga ggcccaggcc agcttgatga aaaacagatt gaaggcgaag ttcatgccgg
     5641 aagaacgtac gcgggttttc cactcgccgt agtcatcggc aaaggccatc aatgagaagt
     5701 gcagtggcaa tgtgaagcca agaatcacgc cattaccgag gatcaccgcc agccacagcg
     5761 tttgataagc cgggccagag ggcaggaacc acatcaacac cgccagcgca gcgaggagca
     5821 ggttggtgta gtagtaaatt ttgacggtat cgaaacgccg cgttaatggg ctgacaatca
     5881 ccgagccgat aatggaggcg aaggtgacca tggtgaagaa cagcgacgtg tagcccgtac
     5941 tgccttgtaa gacgtaggta atgaagtaca tatacccacc gccgcgaatg ttaaagacgt
     6001 taatcagcag gaaagacatg accagcatca gcagtagttg gtcgttgttt cgcagcccgg
     6061 caagatgttc gcgcagggta aatttcccca ttgtggagag tggcacccgt tcacgaaccc
     6121 agaagaaaca gcacaggaac atcaccacgg caatggcgca caatacgccg acgcccagtt
     6181 gatagccgcg cgcggcatta ccctgaccga agagatctac catccacggt aagcctaccg
     6241 aaaccagaaa tcccgccacg ccgcagagaa cgaatcgcca ggactggcag gagatcactt
     6301 cattgtggcg ggtggtcatg gtgttgatca gcgcgcagta cggcacgttg atggcggtat
     6361 aaccgactga aaggagcagg taagtgccga acgcccaggc gatttttact cccatgctgg
     6421 catctggcac ggtgaacgtc aggatgccga taatgccgat gggaatggca atccacaact
     6481 gccacggacg aaagcgcccc cagcgacttt gcgtccggtc ggcaatgacg cccatcaccg
     6541 ggtcggagat ggcatcaaac acgcgcagtg caataaacag cgtgccgacc agcgccggag
     6601 ttaagccaaa aacgtcggtg tagaaaaagg tgagaaagtt catgatcagg caagttatta
     6661 cggtgccgcc tgcgtcaccc aggccgtagc ctattttttc ccgtacagat aacgcatcgt
     6721 caattttacc tttagcgata tcagcggtag taatcggtgt agaggtcata gatattcctc
     6781 gtgaataggg agcaattttt agttttattg caatgacctg attaattctg ccgtgaacgg
     6841 gaatatcaac aaggcgagaa aagagtaaaa aaaggtgacg attccgacct gaatttaaaa
     6901 gtgtgaaatg gatcaggtga aattaatgtt tttgattaat aatcaaaagg taatgtgttt
     6961 aatcattaat gaataagcgg gaaattacat atcaatgctc gaattatcca tgacgttacc
     7021 gattaaagtg caaaatggtg gattatttat ttcccgtggc gtagggaggc atccggcaag
     7081 gcggctggag tcatgggaaa ttatctttgt cgaaaaaggt cgattagcga ttcaggaaga
     7141 agagcggata tttctggtcg atgcgggaga gagtttatta ctctggccga accgacagca
     7201 tgttggggta gaggattttc ccgccgatct caaattttac tggctgcatt ttgaggttaa
     7261 agcgccggat agcgacccgc gctggctcac tcatcttagc gtgccgcaac aaacgaaggt
     7321 tgccgatccg caggcgctgg tggccctgtt tcgccagttc ttaagcgaac aggaaaagca
     7381 tcaacgcact ccggcgctgg agtttattgt gctgctgatt ttgcagcagc tcacccgtga
     7441 cgcgcgtctt gatgaacagg ccgatgccgc aggtaccacg cttgcctgga aagcgcagaa
     7501 actgatccgc acgcaatatc atcgcccgct ttctacggcg cttttagccc gcgagctgta
     7561 ctgcaatgcc gattatcttg ggcgtgtcta tcggcgggtg tttcatctga cgttaacaga
     7621 agcaattcat cgccagcgag tgcgcgaagc cgaaaagctg ttaattagcg aagcgcgatc
     7681 gctcactgaa gtggcggcgt tgtgcgggtt taatgacgtt ggctatttcc ggcagatttt
     7741 tcgcaaacac accggtttaa cgccagctgc gtggaagcga cgttacagca aggaacatat
     7801 caattcgtag tgccggggca atgaagcccc ggcgtgaggg attactgccc gtaataggca
     7861 ttggccccat gcttacgcag gtagtgctta tccaacagtt cgttttgcat cgcaggaagc
     7921 tgcggcgcaa gctgacgcga gaacaggccc atataggcac actcttccag caccacggcg
     7981 ttatgcaccg catcggcggc attttttccc caggcgaacg ggccgtgaga atgtaccaga
     8041 accgccggga tttgcgctgg actcctgccg cgttcttcga gggtttcaat gatcacttcg
     8101 ccagtctggt attcatactc gccgttgatc tcttctgcgg tcatcaggcg cgtgcagggg
     8161 atggctccat agaagtaatc ggcgtgcgtg gtgccccagg cggggagatc cagcccggcc
     8221 tgtgaccaga tggtggcgtg gcgcgagtgg gtatgcacaa tgccaccgat ttcggcatag
     8281 caacggtaga gcgccagatg cgttggcgtg tcggacgagg gctttttgct gccttccacc
     8341 accttaccgc tggctatctc aaccaccacc atatcgtcgg cggtcatcac gtcgtactcg
     8401 acgccggaag gtttgattac catccattgc cgcgtttcgt ctaccgcgct gacattgccc
     8461 caggtgaacg tcaccagatg gtgagcggga agtgccagat ttgccgccag cacgtcggct
     8521 ttcagttgct ctaacatata aatccagcct cctgcatacg cgcttcaatc cagcgccgcg
     8581 cctgaataat ctccagcacc ggctctttgg ctttttcggt ccacatctca atcaggaaag
     8641 aaccgcgata gttcagttca tgcagcgttt tgaagatgcc aacgaaatcg acgcagcctt
     8701 cgccaaacgg cacatcacgg aactgtccgg ggctttgccc ggtgactggc cgggtgtctt
     8761 tcaggtggat tgcggcaata cggtcaatgc ccagtttcag ttcggcggga acatcattgc
     8821 cccaggcgct gaggttgccg acgtccgggt agacggtgaa ccacggcgag gcgagcatct
     8881 cgtcccattt tttccatttg ctgatggagt tcataaacgc ggtgtccata atctccactg
     8941 ccagcatgac ttgtgatgcc gctgcctgtt caaccgccca cgccagccct tcagcaaacc
     9001 gttgctgggt gccttcgtca tggtcctcgt aatagacgtc gtaacctgcc agttgaatag
     9061 tacgaatgcc gagatcgcgc gccaggcgaa tcgctttact catgatctcc cgcgcccgct
     9121 ggcgcacggc gtcgtcccgg ctaccaaaag gaaaacgacg atgtgcggac aggcacattg
     9181 acggaatagc aacacccgtt tcgatcatcg cagtgaccag cgaagcacgt tgtgcggcgc
     9241 tccagtcaag acgtgacaga cgctcgtcag tttcatccac cgacatttcg acaaaatcaa
     9301 agccgcagct ttttgccagc accagccgct ccggccagga gagatctttc gccagcgctt
     9361 tttcataaat ccctaacgga tgattacgca cgcgcgcctc cccagatagc gtcgatttgc
     9421 gcatggaaat cgccagccac ctgcaccgga tttgctgcgc ctgccagcgc ccgtccggca
     9481 ataaacgcct ttacgcggat atctttaaac agtggcagat cagcaggagt aataccgccg
     9541 gtaatggaaa gctcaaggcc gatatcggaa agcgccttca tgcgtgcaag atcggcttcg
     9601 ccccactgtt gcccgctggc ttgcgcgtcg cgaccgcgat gataaatcgc ctgccgcacg
     9661 ccaatacgat gccagtcgcg ggcgtcgtcc agcgtccagt taccgaacag ctctatctga
     9721 atttcaccgc cgcagcgttg cgccatcgcg tggccttttt ccaccgtcgc gagcggtgcg
     9781 gcgcagatga tggtcatcca gttagcacct gcggtaaatg cttgttgagc gagcgtttca
     9841 ccggcatcgg cgaccttcca gtcagcgacg atgattttgt tcgggcactg ttcgcgcaag
     9901 gctttcactg cgccaagccc ttcgtttaaa cagagaatgg tgcccgcttc gacgatatcg
     9961 acgctgtctt ttaacagcat cacgtcgcgc tgtgcgtctt caagtgatga gtggtcgagg
    10021 gccagttgca gaagtggtcg gctcataatg tgtgctcctt gatgcgggcg tgaaagccct
    10081 gaagtgcggc aatgagatgc tgatagcgtt ggtatttttg ttggtaaagc tgatgggcgg
    10141 tcatatccgg cagcagggtg cgtaccgggt gctgtaaatc acgttgggct tcgctgaaat
    10201 cgcgataaac cccggtgccg acgcgggcgg caagcgccgc gccaaaacag ccggtttctt
    10261 ccacctgcgg cagttcgata cgcagcccgc tgacgtccgc cagcatttgc atccagacat
    10321 cggaatgcgc cgggccgcca gtgacgcgca tggtgtgcac atcagtaaaa cgttcgcgca
    10381 ttcggttgag atgggtcata tggctgaaca ccacgccttc ataaatggcc tgcaacaggt
    10441 gtgcgcgggt gtgaatggcc tgcatcccgt agaaaccgct ggtcatctcg agtccggcgt
    10501 tgctgccgta caggaacggc aggaaaaaga gatcgccccc ggccttcggc aagctggcaa
    10561 cggcctggtt aatctcatca aacgagattt ctccccactg tgcggtaaac cattcgaggt
    10621 tgccggaaga ggtggggctg gcttcgtgaa cgataaattg accatcgtta acgtagcgac
    10681 catagacata cggatgcgct tcaccgtcac gtaaaccacg ggttattccg ctggtcaccg
    10741 cccaggtgcc catcaccgca ttaagggtaa attcgtcttc gatcccggca cagagtgcgg
    10801 tggaaaccac atcaaacagg ccgccgacaa cgggcgtacc cgctttcaga ccggtcagtg
    10861 cggctgtctg agcggtgatt tccccgcaga tttcggcaga tccgacaaca ggcggcaggg
    10921 cgtggttgat ttcagcgatc cccagccagt cggtgagaca cgggtcatat tccccaagac
    10981 tcatgttgta gagattggac tcggaaatat tgctctcttc acagcctttg acgccagtta
    11041 aacaccagcg caggtagtcg tgcgtcatca tcacgcagcc aatttgcgcg tagcgttctg
    11101 gttcgtgctc tttcagccag cgtaacagcg acaccggatg cccggtccac aaggtttgtc
    11161 gggtcagcgg gtagagtttt tccgggatgc catcttcctg ccagcgacga acgatttcca
    11221 tcgcccggcg gtccgaggac aaaatagcat tcccgagcgg cttgtcgttt ttatccagca
    11281 aaaacaagcc ctttccctgt gcggagatgc cgataccgac tatttgttcc ccgctaacac
    11341 cagaatgagt aagcaggggg cgaatgacag ccatgcagca ttgccacagt tctgccatat
    11401 cgcgctctgc ccagcctggc tgcgggctta atgcgcacag cggcaggcgt tgcacgcctg
    11461 cctcccggcc ttcgcggtca tacagcccgg ctttcagcca gctaccgcca caatctaacc
    11521 ccagccagta ttgcgtcatg gtttattgca cctcatccac ggctttcagc agatcagctc
    11581 cctgcggcac atctttaaca aacatgtcgc gaacctgatt ccccagtgcg tcgctaaagg
    11641 ctttgcggtc gagatcggtg atgacttcca cgcccgcttc tttcatgccg tcgatgattt
    11701 tttgctgatc ttcggcaacc agtttgcgct ggtagttacc cgcttcctgc gcagatgaga
    11761 tcagcgcctg ctggaactcc ggggttaagc cgtcaaactt cgctttgttg atcaccacca
    11821 gaagtggcga ataggcgtgg tgtgtcagag aaaggtactt ctgtacttcg aaaaattttg
    11881 ctgaccagac gacgttgatc gggtgttcct gagcgtcgat agtgcgcgtt tccagcccgg
    11941 tatagacttc ggcaaacggc atcgggatcg ggttagcgcc aaagactttg aatgcggcga
    12001 tattcatcgg gctattgttg gtgcggattt tcaacccttt caggtctgcg ggggttttaa
    12061 ctggtgcgcg ggagttggtg acatcgcgcc agccgttttc ccagtaggca agtactttca
    12121 gtcctttacc ttcaagtgag gctttcaaat catcaccgac tttgccgtcg agcgttttat
    12181 gcgcgtgggc ggtatcgcgg aacaggaaag ggacatcaag caagttcatc actggtgata
    12241 acccagcaaa gttattcgag ccggacattt ccatatcgat ggtgccgcca cgtacgccgc
    12301 tgatcatcgc ctgcgcgtta ccgagagtgc tgtccgggaa cagttttaat ttcagctcgc
    12361 ctttggttct ctcctgcaat aaatcattga attttttcgc cgcaatatgt tgcgagtcgg
    12421 tttgtgaggt ttcataaccg aaacgtaaag actgcgccgc cattgaagat gtactaaatg
    12481 cagccaggcc agcaatgaat aatgcgtagg ttacagagcg taatttcata atattttcct
    12541 tttaattaat ccatttcaaa gggaggatga tgagatcggg aataaaaaca aatacgacta
    12601 ataatgaata taaaaccagg acgtaaggga aaacgcctct gactgcatca tcgaatttga
    12661 gttttgccac ccctgaaata acgttaagca cattaccgat aggcggtgta attaagccga
    12721 ttgagcagtt aatgataaac atgacaccga aataaatcgg atcgattcct gcctctttaa
    12781 ctaaaggcat taataccggg gtaagaatta ataccgtcgg cgttaaatcc atgaccatgc
    12841 cgacaatcag gatagccacc ataatgacga taaacagcag acgcggtgaa tcgaccagcg
    12901 gttgcagtaa atcagaaacc atcatcggca gttcagcaat ggtgatcagc caggcggaaa
    12961 cttgtgccga ggccaccaga aacatcacca ctgaggtcgt tttcgctgcg ccaatgagca
    13021 catgccaaag tgtggcgaag gtcatttcac ggtaaataac tgtggcgaca aacagcgcat
    13081 agaaagcagc aacggccccg gcttcggtag gggtaaacag accggagcga aacccgccaa
    13141 taatgatgac tggaagaaac agcgcccaga taccggagac aaaagagtgc cagatttcct
    13201 gcatcgttgc tttttgctgg cggggtaaat taaggcgact ggcctgccac caccaggtaa
    13261 gcatcagcgt tgcgcccatc atcatgccag gggcaatgcc cgccataaac agcttactga
    13321 tggataatcc gctggaaacg ccgaaaataa taaacggaat ggaaggggga ataattggcg
    13381 cgataatgcc gccagaggca atcagccccg ccgcccggtt gaccgggtag ttggcgctgc
    13441 gcatcatcgg caccagtaga gcggcaacgg cggcggtatc cgcaacggca gaaccagaaa
    13501 ggcttgccat aatcatagcc gccagcacgc cgacgtagcc cagcccgcct ggtttatgcc
    13561 ccaccagttt catcggcagg tcaacaatgc gttttgacaa gccgcccgca ttcatgattt
    13621 cacccgccag cacaaagaac ggaatcgcca gcagggagaa gctgtcggct ccgttcacca
    13681 gcgtttgtgc catgatctgg acatcaaaca tgtccagcca gaacattaac gccgctccgc
    13741 acaacaacag tgcccaggca ataggcaaac cgatagcaat accacccaac agacagccca
    13801 gaaaaatcag cacagccatg attaagctcc ttgcggtggc gacgttagag aattgctacg
    13861 cgtgatgagt tgatataaat gacgcagttc aaagaatgcg ataacgaggc tggtgggcag
    13921 acaagcggca tacatcaggc cgataggcaa accgaggatc ggtgaataat cgctccagtc
    13981 ctgaattgtt tttagcgttg cgccccaggc cagtgcgcca caaataaata agattaagga
    14041 atgggtaacc agagcgactc gtcgctgcca tgcgggggag agtttctcca ccagaaaggt
    14101 gacctgaacg tgggcgttat ccataaaagc cacaatcgcg ccaataaatg ttaaccagac
    14161 aaataaataa cgtgacaatt catctacaga taaaatgctt gtctgaaaac catatcttaa
//
LOCUS       DACBVI010000116        13837 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.116, whole genome shotgun sequence.
ACCESSION   DACBVI010000116 DACBVI010000000
VERSION     DACBVI010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.116"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..1106
                     /gene="hcp"
                     /locus_tag="IU325_16740"
     CDS             <1..1106
                     /gene="hcp"
                     /locus_tag="IU325_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HAP5810647.1"
                     /translation="GEQQGCISSRCGTSASIGNRWQIGHEDEIFAFSLSNSITNTGKG
                     SQLHGLSFCKLIDKSSPLLINAINNNEQLFMEFDFYRINRFGRWEKYYNIQLRGALLS
                     AINHLFTENNLDTETITVSYEYILSRHLIANTEFSYLAFPDNYNRLFIPRPKTKDDNG
                     LKTLNSKAVGRLLAAGGIYNGNIEGFRETANKLGGDAPAGYDQVMDNKGLLIAGASIV
                     AGVVLGRMRFPDLESLEHFGARGAVSGRPFNPDLAGGPIENLTIDGVTINREGIAIVE
                     KHISRFDHDPANDVMISRLKKIANKELLPEKYDLNYYTHECREYQRYCNLGWETGEPK
                     GLDGYELWNNVHTATLEDFKIKDTDLFHPDATK"
     gene            1103..1360
                     /locus_tag="IU325_16745"
     CDS             1103..1360
                     /locus_tag="IU325_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP5810648.1"
                     /translation="MTITIVEFNVLKVMAEKDIDWSWMVLDRTLAIRNIPGFGNVANI
                     VTKLVNHGLVDIVNGEGNSRPRYRVSQYGLNLIKEQQDNLF"
     gene            complement(1849..4110)
                     /gene="metE"
                     /locus_tag="IU325_16750"
     CDS             complement(1849..4110)
                     /gene="metE"
                     /locus_tag="IU325_16750"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP5810649.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLDEVDEALALGHKVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPIYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRGDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            4228..5181
                     /gene="metR"
                     /locus_tag="IU325_16755"
     CDS             4228..5181
                     /gene="metR"
                     /locus_tag="IU325_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HAP5810650.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            complement(5069..5968)
                     /gene="bioP"
                     /locus_tag="IU325_16760"
     CDS             complement(5069..5968)
                     /gene="bioP"
                     /locus_tag="IU325_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HAP5810651.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWIGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(6044..6844)
                     /gene="yigL"
                     /locus_tag="IU325_16765"
     CDS             complement(6044..6844)
                     /gene="yigL"
                     /locus_tag="IU325_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HAP5810652.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            complement(6852..7874)
                     /gene="pldB"
                     /locus_tag="IU325_16770"
     CDS             complement(6852..7874)
                     /gene="pldB"
                     /locus_tag="IU325_16770"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HAP5810653.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7985..8605
                     /gene="rhtB"
                     /locus_tag="IU325_16775"
     CDS             7985..8605
                     /gene="rhtB"
                     /locus_tag="IU325_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HAP5810654.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            complement(8667..9287)
                     /gene="rhtC"
                     /locus_tag="IU325_16780"
     CDS             complement(8667..9287)
                     /gene="rhtC"
                     /locus_tag="IU325_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HAP5810655.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRSALKKEVVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIIET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(9351..11180)
                     /gene="recQ"
                     /locus_tag="IU325_16785"
     CDS             complement(9351..11180)
                     /gene="recQ"
                     /locus_tag="IU325_16785"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP5810656.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGIGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            complement(11313..12182)
                     /gene="pldA"
                     /locus_tag="IU325_16790"
     CDS             complement(11313..12182)
                     /gene="pldA"
                     /locus_tag="IU325_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HAP5810657.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYIIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGTSYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYNFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            12347..12814
                     /locus_tag="IU325_16795"
     CDS             12347..12814
                     /locus_tag="IU325_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HAP5810658.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            12866..13756
                     /gene="rarD"
                     /locus_tag="IU325_16800"
     CDS             12866..13756
                     /gene="rarD"
                     /locus_tag="IU325_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HAP5810659.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPISLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRTSK"
BASE COUNT         3463 a         3603 c         3454 g         3317 t
ORIGIN      
        1 ccggagaaca acaaggatgt atttcctctc gttgtggaac gagcgcatca ataggtaatc
       61 gctggcagat tggccatgaa gatgaaatct tcgcgttctc gctctctaat agtattacga
      121 atacaggcaa agggtcacaa ttgcatggcc ttagtttctg taaattgatt gacaaaagct
      181 ccccgttact tattaacgct attaacaata acgagcagtt gttcatggag tttgacttct
      241 atcggatcaa tagatttggt cgatgggaga aatattacaa cattcaactt cgcggcgcgc
      301 tcttatcagc aatcaatcac ctgtttactg agaacaatct ggatacagaa accatcactg
      361 tcagttatga atatatactc tccaggcatc tgattgctaa tactgaattt agttatttgg
      421 catttcccga taactacaac cgtctgttta taccacggcc aaaaactaaa gatgataatg
      481 gcctcaaaac actcaatagt aaagcggttg gcagactgct tgctgctggc ggcatataca
      541 atgggaatat cgaaggattc agggaaacag caaacaagtt gggaggagat gcacctgctg
      601 gttacgatca agttatggat aacaaagggt tactcatcgc tggtgcttca atagttgctg
      661 gagttgttct gggaagaatg agattccctg atttggaatc acttgagcat ttcggagcca
      721 gaggagcagt atcaggaaga ccatttaatc ccgaccttgc cggtggtcca attgaaaacc
      781 tgacgataga tggcgtaaca atcaacaggg aaggtatcgc tattgttgaa aagcatatct
      841 ccagatttga tcatgatcca gctaatgatg taatgatttc aaggctaaag aaaatagcaa
      901 ataaagaatt gcttcctgaa aaatacgatc tgaactacta tactcatgaa tgtcgcgagt
      961 atcagcgtta ttgtaattta ggatgggaaa caggtgaacc gaaaggttta gatggatatg
     1021 aattatggaa taatgtacat actgcaactc tagaagactt taaaattaaa gatactgact
     1081 tatttcatcc ggatgcaaca aaatgacgat tacgatagtt gaatttaacg tgctgaaagt
     1141 tatggctgag aaagatattg actggagctg gatggtgctg gatcgtactt tagctatacg
     1201 aaatattcca ggatttggca acgtagcgaa tatcgtaaca aagctggtta atcatgggtt
     1261 ggttgatatc gtaaatggtg agggcaactc tagaccacgc tacagagttt ctcaatatgg
     1321 attgaatctt atcaaagaac aacaagacaa cctcttctga tccagccatt acaggagcat
     1381 gatttacatt gtgatctttc aataagatac tcatcaattt tggtacagaa tatcgtgatc
     1441 actaaaaatg aacttaacgt gctgaacgtt atgactgaga aagatataaa ctggaactgg
     1501 atgaatctcg atcgcacact atccataagg caaattccag gattcgggaa tgttgttaac
     1561 attgttaaca aattagctaa cgaaggtttg gttattattg aggatagcgg gaataagtct
     1621 atgcctcact accacgttag tgaaaaggga tacaatctcg tgcaacgtga gaataaataa
     1681 cgtagcgttg attactgaac ggaatcactc aaaactgtgt tttgcgaaaa aaacgattcg
     1741 gttatttctg ctcctgaaga tcagcttcaa tctggcactc atgcaaaatg gcttccagtg
     1801 ctggaagcca ttatcgtact gcaatttaca gtgactcgtt aatattcgtc atccccgacg
     1861 caaattctgc gccgcctgca ccatgttcgc cagtgccgcg cgggtttctg gccagccgcg
     1921 cgttttcagg ccacagtccg ggttgaccca cagacgctct gccggaatgc gttttgccgc
     1981 tttcttcagc aaggcttcaa tccattctac gctcggtacg tttggcgagt gaatgtcata
     2041 gacgccagga ccgatttcat ttggataatc aaactcttca aacgactcca gcaactccat
     2101 gtcggaacgc gaggtttcga tggtgatgac gtctgcgtcc agcgccgcaa tcgaatccat
     2161 gatgtcgttg aactcgcagt aacacatgtg agtgtggatt tgtgtgtcat ctttcgccac
     2221 ggcggcgttg atacggaaag cctccacgcc ccactggaga taggcatccc agtcgccgcg
     2281 acgcagcggt aaaccttcgc gcagtgccgg ttcatcaatc tggatgatgc caattccagc
     2341 ggcttccagg tccgccactt catcacgcag cgccagcgca atttgtttgg cgatggtttc
     2401 acggctgaca tcttcacgcg ggaacgacca gcagagaata gtcaccgggc cggtcaacat
     2461 ccctttcacc ggtttatcag tcagggattg cgcatacttc gcccactcaa cggtaatcgg
     2521 tgccgggcgg ctaacgtcac caataacaat cggtggcttc acgcagcgtg aaccgtagct
     2581 ctgtacccaa ccgttttgcg taaagacaaa accgtcgagg tgctcgccaa agtattccac
     2641 catgtcatta cgctcagcct cgccatgtac cagcacatcc agtcccaaac gttcctgctc
     2701 aacaatggcc tgcttgatat gttccgcaat gcccgtgcgg tagttattgg cgtcgagatt
     2761 acccttttta aaatccagac gcagggtacg aatctccgtg gtttgcggga aggaaccaat
     2821 cgtggtggtc ggccacgcgg gcagtttaaa acgcgcacgc tgggcttcag cacgcacttc
     2881 atagacattc gcacgctgac tgtcctgggc ggtgatcgcc gccagacgct tttctactgc
     2941 cggattatga acacgagtag agtgacgacg cgcctgaatc ggagcgctcc actctgccag
     3001 agcagccgtg tcaccactgt tcagcgcatc gcgcagcagt gccagttcat gacatttttg
     3061 cagggcgaag gcaaaccagc ttttcacttc tgcatcaaga cgcgtttcca cgctcaggtc
     3121 gatggggctg tgcagcagtg agcaggaaga tgccacccac aaatcgcgtt tgccgacaat
     3181 gtccttaatt tgcgcatact tctcggtaag atcggcgcgc cagacgttac gaccattgat
     3241 cagacccgca gacagcagcc agtcagaagg cagacgcttg tgcagttcag caacgtcatc
     3301 tttaccatgt acaagatcga catgcagacc ctgaacaggc agcgcagtaa tcgtgtcgag
     3361 atttggcgtt acgccttcaa aataggtggt cagcagcagt ttcacctgtc cctggagcgc
     3421 gtcgtaagcg ggtttgtatg cgtccagcca cgcctgcggc agttccagta ccagcgcggg
     3481 ttcatcaatc tgtacccact caatgccgcg ttttgccagt tctgccagca cttgctgata
     3541 aatcggaaga atgtcgttca gcaggctcag gcgatcaaac tgttcacctt tcactttacc
     3601 cagccacagg taggtaaccg gccccagcag cacaggtttc accttgtggc ccagcgccag
     3661 cgcctcgtcc acttcgtcca gcagctgcgt ccaggtcagt ttgaactgtt ggcctttaac
     3721 gaattccggc accatgtagt gatagttggt attaaaccat ttggtcattt ccgctgccgc
     3781 cgcaggttcg ccagtcggcg cacgtccacg accaatacgg aacagggtgt cgatatctac
     3841 cgaaccatct ttgttctgat gacgcgccgg aacgttaccc agcagcagac tggtggtcag
     3901 tacatgatcg taccaggcaa aatcgcccac cggcagcagg tcgatacccg cttgcttttg
     3961 ttgatcccag tgacgagcac gcaattcacg ccctaccgcc agcagttctt cacgcgtgga
     4021 gttccccgcc caataactct cttgcgcttt tttcagctcg cgacgcaggc caacgcgagg
     4081 gaaaccgagg gtgtgattaa gaattgtcat tatatgcccc ttatggaatt tttagtattt
     4141 agccgtccag atgtttacac atccataatt ggcggttact gtatattcct caagcgcaaa
     4201 ttattcatgc cgaagtgaag gactttcatg atcgaagtaa aacatctgaa aacgctacaa
     4261 gcgttgcgga actgcggctc gctcgcagcc gctgcggcga cgttgcatca gacgcaatcc
     4321 gccctgtctc accagtttag cgatctggaa caacgccttg gcttccggct atttgtgcgt
     4381 aagagccagc cgcttcgctt tacaccgcag ggagaaatcc tgttgcaact ggcaaaccag
     4441 gtgctgccac aaattagcca ggcactgcaa gcctgcaatg aaccgcagca gacgcgtctg
     4501 cgcatcgcca ttgagtgcca tagctgtatt cagtggctga cgcccgcgtt agaaaatttc
     4561 cataagaact ggccgcaggt cgagatggac tttaaatcgg gcgtgacgtt tgacccgcag
     4621 cctgcgttgc aacagggcga gctggatctg gtgatgacgt cagatattct gccgcgcagt
     4681 ggcctgcatt attcgccgat gttcgattat gaagtgcgtc tggtgttggc acctgaccat
     4741 ccgctggcgg caaaaacgcg tattacaccg gaagatctcg ccagcgagac gctgttgatt
     4801 tatccggtgc agcgtagccg actggatgtc tggcggcatt ttcttcagcc ggcaggcgtc
     4861 agcccgtcac tgaaaagcgt cgataacacc ttattgttga ttcagatggt tgccgcgcgg
     4921 atgggtattg ccgccctgcc gcattgggta gtagagagtt ttgagcgcca gggtctggtg
     4981 gtgaccaaaa ccctgggcga aggcttgtgg agccgactgt acgccgccgt gcgcgatggc
     5041 gagcagcgtc agccgattac tgaagcgttt attcgctcag cgcgcaatca cgcctgcgat
     5101 catctgccgt ttgtgaagag cgcggagcga cccacttacg atgcacccac agtgaggcca
     5161 ggatcaccag cgcgcctgta atgaacgttg gccagtgcgg ctgttggtgc cagatagcca
     5221 ggtttaccag cagccctgcc ggaacgtgca tattattcat aatacccagc gttccggcgt
     5281 ctacttgggt tgcgccgtag ttccacataa agtagccaat cccggaagcc accacgccaa
     5341 gaaacaccag aatgccccat tgcaacgtgg tttgtggcat tttctgcgca tttcccagca
     5401 agaaccatgc aatcaccgcc accaggaacg cgccaagata gaaccacgca aaggcgttat
     5461 gctgcggcat cgggcgagtt tccatcagac gtttgtaacc caccatgccg atggcaaaag
     5521 tgatattgga gagctgcacc agcagcaggc caatccagaa gtggtcggtg acctgatcat
     5581 agcgaataat cccggcacca atcaccgcca acaaggcgct aaaagcatag ccccagcgca
     5641 gacggcgctt actcatgatg tcataaatca acgtaatgta gagcggcgtc agtacggtga
     5701 acagcaacaa ttcggagacc gtcaggtaga gataggcgcg aaaactcagc atatacatca
     5761 cgccgagctg catcgcgccc accagcatat acaggccgac cgttttcagg ctattgccac
     5821 gggtacgcag aaacggcaga aaaacgagtg ccgccaggcc aacgcgcacc agcaccgcaa
     5881 aatagctatc gacgtgcccc gcaaggtact cgccataaaa gctaaaggag aaggcccaca
     5941 gaatcgtggt gatgataagt agcgccacaa tggaaaactc tttgattaac gagtatccat
     6001 tgtagcgaag tatcaggttg acaactggcc aaataaagaa cgattacgat aaatagagct
     6061 tacgcagata atgcggcacc gcgtcgtcgg cattagtacc aatcacttcc agctccggat
     6121 gcagatcttt cagacgctgg tgcgcactgc ccataatgca gcctttcccc gccatcgaca
     6181 gcatttcggc gtcgttcatc ccgtcaccaa aggcaataca atccttcagg ctgtagccca
     6241 gtttcttcgc caccgcttcc agcgcatgac ctttcgacac gccgcccgcc atcacttcca
     6301 gacaggttaa ggtagagaaa ctaacgttga cgcgatcgcc ccagcgagcg ttgatcgcct
     6361 gctccagcgg cagaagttgt tcatgggaat cgcaggtgaa gaacactttg ctgacgcctt
     6421 ccggctccag taatcccggc tcatacagcg catatttgaa caccgcttct ttaaaaaagc
     6481 gcatctcttc cgggcgatgg cgattcataa accattcgtc gtcgcgataa acgttagtaa
     6541 tgatgtccgg attgtcgttg actacgccaa acagatcgct ggcaatgtcg cgatccaggt
     6601 tatgagcaaa aatcagattg ccatccagat cgtgaacgcg cgcgccattg gaggtaatca
     6661 tgtaagactt aatctccaga ttatcgcgaa tttgccccac atcaacgtgg tgacgaccgg
     6721 tcgcaaacac aaagttgatg ccgcgcgcgg tgagcagctt cagagtttct ttggcgtaag
     6781 gggataacgt atggtcggga gaaagtaacg tgccatctaa atcagacgca acaacctggt
     6841 acataagaaa tttaaacctc tgtagagcgg tttccgctgg gtgagttatg cctgttgaaa
     6901 aaatcaacga tggcgtggag cgcgactgag cgcattgcgt ccttttcaaa aaggatctca
     6961 tggtaagcac ctttaattac caacggccgt cctccttcga caggatggcc cgcggcggtg
     7021 cggagttcac aaaaacggtc gtgcatgcgg ttatccacca cgcgttcctc ttcagcttgt
     7081 aaaagaagcg ttggcgtggc gtcatcaccc gcaccggcta acacctgttc gccagccaga
     7141 atactttcgc gtacccaatg gtaggttggc ccaccgacgc gaatcgttgg gtcatcagca
     7201 tagaagcgta agttacgtcg atatcgctgt ctgctgtggg tcagtacgtt gatagcaaac
     7261 ggcaacgcgc gccagcgccc ggtgcctatt gcatagccat cacggaaacg tggatgcgct
     7321 tcggcccaat tgaggatctg ccgtgccata aatgacggca tacgaatcac gatcccaaac
     7381 attggcgcag ttagcgcaat agcgtcacat acacctggat ggcgttgcag aaataatgtg
     7441 gagatcgcac cgcccatcga atgtgccagt atatagcgtt tacgccacgg accgggctgg
     7501 acctcctgtt gccagaatgc cgccagatca tcaacataat cattaaagcg attaacatgc
     7561 ccgagatgcg gatcggctaa caggcgaccg gaacgtccct gcccgcgatg gtcgatgatt
     7621 aagacatcaa accccaaatg gaacaggtcg taggccagtt ccgcatattt tacgtagctc
     7681 tcaatacgcc ccgggcagat gactaccacc cggtcatggt gctgtgcgcg aaaacggaca
     7741 aagcgcaccg gaatgtcatc cacaccagta aactctgctt catctcgctg acgccagaaa
     7801 tcagtcagcg gtcccatggt aaaagcagca aacgcgtttt ctcttgtttc ccagtctttt
     7861 tgctgctgaa acatcgggta atctgcctct taaaccacgt aaaatcgttt ttttaagcgt
     7921 gcctgacaca acgctgcgac agtagcgtat tgtggcacaa aaatagacac accgggagtt
     7981 catcatgacc ttagaatggt ggtttgccta cctgctgaca tcgatcattt taagcctgtc
     8041 gccaggctct ggcgcaatca acaccatgac cacctcgctc aaccacggtt atcgcggcgc
     8101 ggtggcgtct attgctgggc ttcagaccgg actggcgatt catattgtgc tggttggcgt
     8161 ggggttgggg acgctatttt cccgctcagt gattgcgttt gaagtgttga agtgggcagg
     8221 cgcagcttac ttgatttggc tgggaataca gcaatggcgc gccgctggtg caattgacct
     8281 taaatcgctg gcctctactc aatcgcgtcg acatttgttc cagcgcgcag tttttgtgaa
     8341 tcttaccaat cccaaaagta ttgtgtttct ggcggcgcta tttccgcaat tcatcatgcc
     8401 gcaacagccg cagctgatgc agtatatcgt gctcggcgtc acgactattg tggtcgatat
     8461 tattgtgatg atcggttacg ccacccttgc tcaacggatt gctctatgga ttaaaggacc
     8521 aaagcagatg aaggcgctga ataagatttt cggctcgttg tttatgctgg tgggggcgct
     8581 gttagcatcg gcgaggcatg cgtgaaaaat aatgtcggat gcggcgtaaa cgccttatcc
     8641 gacttactct gatgacgcgt ctggcatcaa cgcgaaataa tcaaatgaat gccaaatccg
     8701 gcaaataacg ccccggcaaa accatcaatc cacttcgcca gacgttgata accacggcgc
     8761 atttgcggca gggcaaacag gctggcaacg acggtaaacc acgccagtgt ttcgataata
     8821 atcaacgcga aaatgcccca gcgctcagtg gtgccgacgt tatcgccgac aaacagcgag
     8881 aataccgaac caaagtagat aatcgctttc ggattggcga gattggtcag taagcctttc
     8941 aggaaactgc gtccgctttt tgccagttct acctgtggcg caggcgcaga aacgacctct
     9001 tttttcagtg cgctacgcag catctgataa cccatccagc agagatacag gccaccgccc
     9061 accataatca gcgtatgcag ccaggccatt ttttcgataa tcaaatgtag gccaagcaac
     9121 gcaatcccag cccaaaccat tacgccgcag gtaatgccca gcacgcccat catcgcttct
     9181 ttacgggaac gactaacagc ggtctgagag acaaaaaaga aatcgggacc ggggctcata
     9241 agcgccacaa tgtgcaccat ggcgacggtg aggaataaca tcaacataca ttagactcgc
     9301 gggggaaacg tttacggagt cttcatcctg gcactttttt atgctgctga ctactcttcg
     9361 tcatcgccat caacatgcgc acgaatcagc gccataaacg gtttgccaaa gcgttccagc
     9421 ttgcgcatcc caatgccgtt aacgctgagc atttcgctgg cagtgatcgg catctgttcc
     9481 gccatctcaa tcaaggtcgc gtcgttaaac accacgtacg gcgggacatt gctttcatcg
     9541 gcaatcgatt tacgcagttt gcgtaatttg gcgaacagtt tgcgatcgta gttgccgccg
     9601 aaggatttct gcatcgcttt cggtttgagc gccacgatac gtggcacggc aagttgcaaa
     9661 gaggattcgc cgcgcagcac cggacgcgca gcctctgtca gttgtagggc agaatgctgg
     9721 gcaatatttt gcgtcaccag gccgaggtga atcagctggc ggatcacgct cacccaatgt
     9781 tcatggcttt tatcacggcc catgccatag actttcagtt tgtcatgacc atagtcgcgg
     9841 atacgctggt tattagcgcc acgaatcact tccaccacat aacccatccc aaaccgctga
     9901 ttcacacgac caatggtgga aagggcaatc tgagcatcgg ttgaaccatc gtattgtttc
     9961 ggcggatcga ggcagatatc gcagttcccg cacggctcct gccgcccttc gccaaaatag
    10021 tttagcagca ccagacggcg acaggtttgc gcttcggcaa acgcgcccat cgcattgagt
    10081 ttatgacgtt cgatatcctg taactgcccc tgcggtttct cttccagaca acgacgcagc
    10141 cacgccatat ccgccggatc gtaaaacagc atcgcttccg cgggcaggcc atcacgcccg
    10201 gcgcgtccgg tttcctgata ataggattca atgttgcgcg gaatatcaaa atgcaccacg
    10261 aagcgaacgt ttggtttatt gatgcccatg ccgaacgcca ccgtcgccac cacaatttgc
    10321 aggtcatcgc gctggaattt ctcctgcaca tcagcgcgaa cattattttc cagcccggca
    10381 tgataggccg ccgcgctaat tcccttgctt tgcaggcgcg cagcggtgtc ttctactttc
    10441 gcacgactat tacagtaaat aatccccgac ttaccgcgtt gttcctgtac ataacgcatc
    10501 aactgatcga gcggtttgaa tttctccatc agcatgtagc gaatattcgg acggtcaaaa
    10561 ctgctgattt gaatcagcgg atcgttcagc cccagcaggc gcacgatatc ctggcgcgtg
    10621 gtgtcatcgg ctgtggcggt cagcgccata aacggcagcg tcgggaaccg ctggcgcaac
    10681 tgaccgagcg cggcatattc cgggcggaaa tcgtggcccc attgggagat acagtgcgct
    10741 tcatcaacgg ctaataacac cggattccag tgcgccagat gctcaagaaa gttatccagc
    10801 atcaggcgtt ccggcgcgat ataaagcagg cggatttgcc cggtgcgaca gcctgtcata
    10861 acttcaagtt gctgctcgcg ggtttgcgtc gagttaaggc acgccgccgc cacgccgttg
    10921 gcttgcagtt gatccacctg atctttcatc aacgaaatca gcggtgaaac aaccacggta
    10981 aggccgttta gcaataaagc aggaatttga tagcaaaggg attttccgcc accagtgggc
    11041 atgacgacca ggcaatcgcg accggaaagc acagtgtcga taatctcttc ctggccgggg
    11101 cgaaactgtt ggtagccaaa ggtttcttgt aaaacctgtt tagctccgga ctccagattc
    11161 aacacttccg cctgcgccac attcaccccg attgcctgaa ataaaaacag gcgctatttt
    11221 cagcgcctgc gcgagaaact gcaatgcttt tagtctctga gagggtaatc ctggatactg
    11281 gattccagat ttgccaacca ctcaaccgtt catcaaaaca aatcgtttag cataaccccc
    11341 acaccgacac gggtctggtt gaagttatag tcgatgagcg attcgccata gccgctgtaa
    11401 acctgagtat aaaggcgcac atgtttggtg atcgggtaac ttaagcctaa ctccgcgccg
    11461 ccgtagccgg tgttccagtt gtactgtcct ttcgcactca acaccgcatc accgaggtga
    11521 tagccgattt taagctggta atagcccata tatttggtga tgtccgggtt atcgtccgta
    11581 ttccccacca cataccacgg cttcacctct accagccagt taccgttttc tgccatcagg
    11641 cgggtataaa ggcggttcca gctgcgggaa gtcgggtctg aacgcccgtt tgagtcgtgg
    11701 ttgtagccca tttccacatc gcgcagcgtc cagccagcaa agttgtaatc cgtggcaaaa
    11761 ccgaggaaca attgcggttc gtagttagtt tcacgaaacg gtgaagactc ttcgctattg
    11821 gacagttgcc accaggattt ttgcgtataa gacgtaccca acaccgagtt cgggcctaaa
    11881 atcccacgcc acagcggaaa cgccaggctc aactgaaact ttacttcatc cttacgcgca
    11941 ttttccgccc agtcgtaact ggcaatcgct tctttattca gatcgctggt ttgggtatag
    12001 atgatgtagt tggtgtcata aggataaagg gtgaacggat tgtcatgctc ctgcagcata
    12061 ttggcgataa tactgccacg caccgctggc gcgtcatgca cctctttcac cgttgcctct
    12121 tgtgcatata ctgccatagg caacataaac accggcaaca accagccctg cagagtccgc
    12181 atcgctcgtg ttctccgtaa cgaattattg ataaggtgaa tatttttctg cgggctattt
    12241 tacatactta actccatagc cgttagttat cccttcctaa agtagaaacc aacaacaaag
    12301 aagcataaaa tcaacatttc attaactaat gaagtgtaag gatgatatgt ctgccgtact
    12361 gaccgctgaa caagccctga aattagtggg tgagatgttt gtttatcaca tgccatttaa
    12421 ccgcgcattg gggatggaac tggagcgtta cgaaaaagag ttcgcacagc tggcctttaa
    12481 aaatcagcca atgatggtgg gtaactgggc gcaaagcatt ttgcacggcg gggtcattgc
    12541 gtcggcgctg gatgtcgccg ccggtctggt gtgcgtggga agtaccttaa cccgccacga
    12601 aaccatcagt gaagatgaac tacgtcagcg gctatcgcga atggggacca ttgatcttcg
    12661 cgtcgattat ctgcgcccag gcagaggcga gcgttttact gctaccagta gcctgttgcg
    12721 ggcaggcaat aaagtcgccg tcgcccgcgt tgaattacac aatgaagaac agctttatat
    12781 tgccagcgct acagccacct atatggtagg ttgataacgt aaaagcaggt aaactgctgt
    12841 taccaattaa aaatgagtat tcccgatgga tgcaaaacaa acgcggcagg gcgtattact
    12901 cgctcttgcc gcttatttta tttggggtat tgcgccagcg tacttcaagc tgatttacta
    12961 cgtgcccgcc gatgagatcc tgacgcatcg cgtgatctgg tcgtttttct ttatggtggt
    13021 gctgatgagc atctgccgcc agtggtccta tttaaaaacg ctgattcaga cgccacagaa
    13081 aatttttatg ctggcagtct ctgccgtgct gattggtggc aactggctac tgtttatctg
    13141 ggcggtgaac aatcaccata tgctggaagc gagccttggt tactttatta acccgctggt
    13201 gaacattgtg ctggggatga ttttcctcgg cgagcgattc cgccggatgc aatggctggc
    13261 ggtgattctg gcgatatgtg gcgtgttggt tcagttgtgg acttttggtt cactaccgat
    13321 tatcgcgctg gggctggcgt ttagttttgc cttctacggc ctggtacgca agaagattgc
    13381 tgttgaagcg caaaccggca tgttaatcga aactatgtgg ttgctgcccg tggcggcgat
    13441 ttacctgttt gctattgccg acagctcaac cagccatatg gggcaaaacc cgatttcact
    13501 gaatttactg ctgatcgccg ccgggattgt cactaccgta ccgctgttgt gttttactgc
    13561 ggctgcgaca cgcctgcgtc tctcaacgtt aggctttttt cagtacattg gcccgacgct
    13621 gatgttcctg ctggctgtga cgttttatgg tgaaaaaccg ggtgccgata agatggtgac
    13681 cttcgccttt atttgggtgg cgctggcaat ttttgtgatg gatgcgattt atacgcagcg
    13741 tagaacgtcc aaataaaaaa taaatatatt ctgaaaaaat atgccgactc ttgcatgaga
    13801 gtgggcatat tgccatgctg acaggagact cacataa
//
LOCUS       DACBVI010000117        13784 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.117, whole genome shotgun sequence.
ACCESSION   DACBVI010000117 DACBVI010000000
VERSION     DACBVI010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.117"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..510
                     /gene="dosC"
                     /locus_tag="IU325_16805"
     CDS             <1..510
                     /gene="dosC"
                     /locus_tag="IU325_16805"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HAP5810660.1"
                     /translation="RHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDVD
                     KFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLRT
                     AERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGRN
                     RVELWKASL"
     gene            535..2934
                     /gene="dosP"
                     /locus_tag="IU325_16810"
                     /gene_synonym="pdeO"
     CDS             535..2934
                     /gene="dosP"
                     /locus_tag="IU325_16810"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HAP5810661.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKIYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDSLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDFIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGINGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            3192..3773
                     /gene="ddpX"
                     /locus_tag="IU325_16815"
     CDS             3192..3773
                     /gene="ddpX"
                     /locus_tag="IU325_16815"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HAP5810662.1"
                     /translation="MSDTTVLVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVAVGSNHSRGT
                     AIDLTLRDEHGNILDMGAEFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIP
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHAS"
     gene            3787..5337
                     /locus_tag="IU325_16820"
     CDS             3787..5337
                     /locus_tag="IU325_16820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP5810663.1"
                     /translation="MKRSISFRPTLLAIVLATTMPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVRFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            5339..6361
                     /locus_tag="IU325_16825"
     CDS             5339..6361
                     /locus_tag="IU325_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP5810664.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVDNIRQQLGLDQPLYVQFYRYVSDLFQGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            6379..7200
                     /locus_tag="IU325_16830"
     CDS             6379..7200
                     /locus_tag="IU325_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HAP5810665.1"
                     /translation="MLKGSPLTVTGAVIIVLMLLMMIFSPWLATHDPNAIDLTARLLP
                     PSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLVVVAIAGMMGSLLGCLSGVLGGRADA
                     IIMRIMDIMLSIPSLVLTMALAAALGPSLFNAMLAIAIVRIPFYVRLARGQTLVVRQY
                     TYVQAAKTFGASRWHLINWHILRNSLPPLIVQASLDIGSAILMAATLGFIGLGAQQPS
                     AEWGAMVANGRNYVLDQWWYCAFPGAAILLTAVGFNLFGDGIRDLLDPKAGGKQS"
     gene            7197..8183
                     /locus_tag="IU325_16835"
     CDS             7197..8183
                     /locus_tag="IU325_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP5810666.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNHRCACWYPQQEVISV"
     gene            8176..9102
                     /locus_tag="IU325_16840"
     CDS             8176..9102
                     /locus_tag="IU325_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP5810667.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENRELTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDVQQVLTAPAHPYTRLLLDSLPAINKPLEEEWALRKTDLPGNRTLPQGCFF
                     RERCPLATHGCEVRQSLTIREDGREIRCWRAL"
     gene            complement(9158..9589)
                     /gene="osmC"
                     /locus_tag="IU325_16845"
     CDS             complement(9158..9589)
                     /gene="osmC"
                     /locus_tag="IU325_16845"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HAP5810668.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            9934..10149
                     /locus_tag="IU325_16850"
     CDS             9934..10149
                     /locus_tag="IU325_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HAP5810669.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            10251..10388
                     /locus_tag="IU325_16855"
     CDS             10251..10388
                     /locus_tag="IU325_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HAP5810670.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            10545..12242
                     /gene="maeA"
                     /locus_tag="IU325_16860"
     CDS             10545..12242
                     /gene="maeA"
                     /locus_tag="IU325_16860"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate-decarboxylating malate
                     dehydrogenase"
                     /protein_id="HAP5810671.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEETARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            12376..13386
                     /gene="adhP"
                     /locus_tag="IU325_16865"
     CDS             12376..13386
                     /gene="adhP"
                     /locus_tag="IU325_16865"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HAP5810672.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRSEDA
                     AKIVQEKTSGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            13503..>13784
                     /locus_tag="IU325_16870"
     CDS             13503..>13784
                     /locus_tag="IU325_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAP5810673.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
BASE COUNT         3417 a         3455 c         3604 g         3308 t
ORIGIN      
        1 cgccacgaag tcggtatgga tgtactgacg aaattactta accgccgttt cctgccgacg
       61 atcttcaaac gcgaaattgc ccatgccaac cggaccggta caccgctgtc agtgctgatt
      121 attgacgttg ataaattcaa agagatcaac gatacgtggg gccataacac tggcgatgaa
      181 attctgcgta aagtctctca ggccttttat gacaacgtcc gcagtagtga ttatgttttc
      241 cgctacgggg gcgatgaatt tatcattgtt ttgactgaag catctgaaaa cgaaacgtta
      301 cgtaccgcag aacgtattcg cagtcgggta gagaaaacca aactgaaagc cgcgaacggc
      361 gaagatattg ccctctcact ttccatcggt gccgccatgt ttaatggtca tcctgactat
      421 gagcgcctca ttcaaatagc cgatgaagct ctgtatatcg ccaaaagacg aggtagaaac
      481 cgtgttgaac tctggaaagc cagtctttag atgcgccagg atgcagaggt aatcatgaag
      541 ctaaccgatg cggataatgc cgccgatggc atttttttcc ccgcccttga gcaaaatatg
      601 atgggcgcgg tgttaattaa cgaaaatgat gaagtaatgt ttttcaaccc cgccgcagag
      661 aagctctggg gatacaaacg tgaagaagtc attggcaata acattgatat gctgattccg
      721 cgggatttgc gtcccgcgca tcctgaatac atccgtcaca accgtgaagg cggtaaagcg
      781 cgcgtcgagg ggatgagtcg ggagctgcag ctggagaaaa aagacggcag taaaatctgg
      841 acccgttttg cgctatcgaa agtgagcgcc gagggtaaaa tttattacct ggcgctggta
      901 cgggatgcca gcgtagaaat ggcgcaaaaa gaacagaccc gacaattgat tattgccgtt
      961 gaccatctcg accgaccggt gattgtcctc gatccggaac gccatattgt gcagtgcaat
     1021 cgcgcattta ccgaaatgtt tggttactgc attagcgaag ccagcggtat gcagcccgat
     1081 tcactcctta acattccaga attccctgct gataaccgca ttcgtttaca acagttgcta
     1141 tggaaaaccg cccgcgatca ggacgaattt ctgctgttga cgcgcaccgg tgaaaaaatc
     1201 tggattaaag cctctatcag cccggtctat gatgtgctcg cgcatctgca gaacctggta
     1261 atgactttct cggatatcac cgaagaacgg caaatccgcc agcttgaagg caatattctc
     1321 gccgccatgt gcagcagccc gccatttcat gaaatggggg aaatcatttg tcgtaacatc
     1381 gaatctgtac tcaacgaatc gcatgtttcg ctgttcgcgc tgcgcaacgg gatgccgata
     1441 cactgggcgt catcttcgca cggtgcagaa gttcaaaatg cgcaaagctg gtcagcgacc
     1501 attcgtcagc gtgatggtgc gccggcgggg atcctgcaaa ttaaaacctc gtcaggagca
     1561 gaaaccagcg cctttatcga acgcgtggca gatatcagcc agcatatggc cgcgctggcg
     1621 ctggaacagg aaaaaagccg tcagcatatt gaacaactca tccaatttga tccgatgacc
     1681 ggtctgccaa atcgcaataa cctgcacaat tacctcgatg acctggtcga caaagccgtc
     1741 tctccggtgg tctatctcat tggtgttgac catattcagg atgtgattga tagccttggc
     1801 tatgcgtggg ccgatcaggc attgctggaa gtggtcaatc gctttcgtga aaaactcaaa
     1861 ccggatcaat atctctgtcg tatcgaaggt acgcagtttg tcctcgtgag cctggaaaac
     1921 gacgtcagta acattaccca aatcgccgat gagctacgga atgtggtcag caagccgata
     1981 atgattgacg ataagccatt cccgctcacc ttgagcattg gcatcagcta cgacgtgggt
     2041 aaaaaccgcg attacctgct ctccactgct cacaatgcaa tggattttat tcgcaaaaat
     2101 ggcggtaacg gctggcagtt cttcagcccg gcgatgaacg aaatggtaaa agagcgtttg
     2161 gttttaggcg cggcgctgaa agaagcaatt agcaataacc aactgaaact ggtttaccag
     2221 ccgcaaatct tcgcagaaac gggtgaactg tacggcatcg aagcccttgc tcgctggcac
     2281 gatcccctgc atggtcatgt gcccccttca cggtttattc ctctcgcaga agagatcggt
     2341 gaaatcgaaa atattgggcg ctgggtcatc gcggaagctt gccgtcagtt agcagaatgg
     2401 cgtagccaga atattcatat cccggcgctt tccgttaact tgtcggcgct gcactttcgc
     2461 agtaatcagc tgcctaatca ggtgtctgat gcaatgcaag cctggggtat caacggccac
     2521 cagctgacgg tggaaatcac ggaaagcatg atgatggaac acgataccga aatctttaag
     2581 cgcattcaga tcctgcgcga tatgggcgtg ggtttatcgg tagatgattt tggcacgggc
     2641 ttttccggat tatcccgctt agtcagtctt ccggtaacgg aaatcaaaat tgacaaaagt
     2701 tttgtcgatc gttgtctgac cgaaaaacgc atccttgcct tacttgaagc cattaccagc
     2761 attgggcaaa gccttaattt aaccgtcgtg gcggaaggcg tcgaaaccaa agagcaattt
     2821 gagatgctac gcaagatcca ctgtcgcgtt attcagggat atttcttttc ccgcccccta
     2881 cccgccgaag aaattccagg ctggatgagc agcgtgttac cgctgaaaat ctgacaaatt
     2941 cctctcgccc gcactcgcgg gttttccttt aacgtgacac tgtcacttaa atactgtgat
     3001 ttcagctacg attccgggag attccttcct taacttcccc tcttgcacca aaactgcact
     3061 acgctgcaca aatgttgatc aaaagttaaa aaatataact ttaagccagc attctggcgc
     3121 ttatcccggc atggcatgag atctgcataa gcggaaagcg cagcaatttt tgtcttatac
     3181 aggaaccgtt tatgtcggat accaccgtac tggttgattt agccgtgatc ttccctgatc
     3241 tggagatcga actgaaatac gcctgcgctg ataacatcac aggcaaagct atttatcagc
     3301 aagcgcgttg tctgttacac aaggatgcga ttactgcgct ggcgaaaagt atcagtatcg
     3361 cccagctgtc aggcttacaa ctggtgattt acgatgcgta tcgcccacaa caagcacagg
     3421 cgatgttgtg gcaagcctgc ccagacccgc aatatgttgt tgatgtggcg gtcggttcta
     3481 atcacagccg tggcacggcg atcgacctga cgcttcgtga tgagcacggg aacatcctgg
     3541 atatgggggc agagttcgat gaaatgcacg aacgttccca tgcttatcac ccttccgtcc
     3601 cgccagccgc tcagcgcaat cgcctgttgc tgaatgcgat tatgactggt ggcggttttg
     3661 tcggtatccc gagcgaatgg tggcacttcg aattacccca ggcagcgagt tacccacttc
     3721 tcgctgatca attcacctgt tttatatccc ctggcacaca gcacgccagt taatcaggaa
     3781 actgtcatga agagatcgat ttcgtttcgt cctacattgc tcgcgatcgt ccttgccaca
     3841 acaatgccgg ttgcgcacgc cgccgtaccg aaagatatgc tggtgatcgg taaagctgcc
     3901 gacccacaaa ccctcgaccc agcggtgaca attgataata acgactggac agtgacctac
     3961 ccgtcttatc agcgactggt tcagtacaaa acggacggtg ataaaggctc aaccgacgtt
     4021 gaaggcgatc tggcgagtag ctggaaagcg tctgacgatc aaaaagagtg gacgttcacc
     4081 ctgaaggaca atgctaaatt tgccgatggc acacctgtca ctgccgaagc agtaaaactc
     4141 tcttttgagc ggttactaaa aatcggccag gggccagcag aagcatttcc caaagattta
     4201 aagattgatg ctcccgacga acatacagtg aggtttaccc ttagccagcc attcgcaccg
     4261 ttcctctaca cgctggcgaa tgacggtgca tccattatca atccggcggt cttaaaggag
     4321 catgcggcgg atgatgcccg tggtttcctc gcgcaaaata ccgctggctc cggaccgttt
     4381 atgctgaaaa gctggcaaaa aggtcagcaa ttagttctgg tgccaaatcc gcattacccc
     4441 ggcaataaac cgaatttcaa acgggtatcg gtaaaaatta tcggtgaaag tgcctcccgt
     4501 cgcctgcaac tctcccgtgg cgacattgac attgccgatg cgctgccggt ggatcaactc
     4561 aacgcactga agcaggaaaa caaagtcaat gtggcagagt atccgtcact gcgcgtcacc
     4621 tatctgtatc tgaataacag caaagcgcca cttaatcagg cggatctgcg tcgggccatt
     4681 tcctggtcta ccgattatca gggtatggtt aacggcattc tgagtggtaa cggaaaacaa
     4741 atgcgcggcc cgattccgga aggcatgtgg ggatacgatg cgacggcaat gcaatacaac
     4801 catgacgaaa cgaaagccaa agccgaatgg gataaagtga cgagcaaacc caccagcctg
     4861 acgtttctct attctgataa tgatccgaac tgggagccta ttgctctggc gacacaatcc
     4921 agtctcaaca agctgggcat caacgtgaag ctggaaaagc tggcgaacgc caccatgcgc
     4981 gacagagtgg gtaaaggtga ttacgacatt gcgattggca actggagccc ggattttgcc
     5041 gacccgtata tgtttatgaa ttactggttt gagtccgaca aaaaaggtct gccgggtaac
     5101 cgctcgttct atgaaaacag tgaggtcgat aagctactgc gcaatgcgct agcgaccacc
     5161 gaccagacgc agcgtacccg ggactaccag caggcacaga aaatcgtcat tgatgacgct
     5221 gcttatgtgt acctgttcca gaaaaactac caactggcga tgaacaaaga ggtgaaaggc
     5281 tttgtgttca atcccatgct ggaacaggtc ttcaatatca ataccatgag taaataacgt
     5341 gaccttctgg agtattttac gccaacgctg ctgggggctg gtgctcgtgg tggcgggcgt
     5401 ctgtgtgata acgtttatta tctcgcacct gatccccggc gatccggcgc ggttactggc
     5461 gggtgaccgc gccagcgatg ctatcgtgga taatattcgc cagcaactgg gactggacca
     5521 gccactgtac gtacagtttt accgctacgt cagcgacctg tttcagggtg acctgggaac
     5581 atccattcgt accgggcgtc cggtgctgga agagttgcgc atatttttcc cggcgacgct
     5641 ggaactggct ttttgtgccc tgctgctggc actcctgatt ggcatcccgc tgggcatact
     5701 ctctgcagtc tggcgaaatc gctggctgga tcatctggtg cgaataatgg ccattaccgg
     5761 aatctccaca cctgcgttct ggcttgggct gggcgtcatt gtgctgtttt atggtcatct
     5821 gcaaattctt cccggcggcg gaaggcttga tgactggctg gatccaccta cgcacgttac
     5881 cggcttttat ctgctcgatg cgctgcttga aggcaacggt gaagtcttct tcaatgcgct
     5941 gcaacatctc atcttaccgg cattaacgct ggcgttcgtt cacctgggaa ttgtcgctcg
     6001 ccagatccgc tcagctatgc tggaacaatt gagtgaagat tacattcgta ccgcccgggc
     6061 cagcggcttg cccggctggt atatcgtttt atgttatgcg ctacccaatg cgttgatccc
     6121 ctcgattacc gtattggggc tggcgctggg cgatttgttg tatggcgcag tgctcaccga
     6181 aaccgttttt gcctggcccg gaatgggtgc atgggtagta acatcaatac aggcgctcga
     6241 cttcccggca gtgatgggct ttgccgtcgt ggtttcgttt gcttatgtgc tggtgaacct
     6301 ggtggtggat ttgctctatt tgtggattga tccgcgtatc ggacgtggag gtggtgaatg
     6361 atgctaagcg aggaatggat gctgaaaggc agcccgctta ccgtgaccgg cgcagtcatc
     6421 attgtattaa tgctactgat gatgattttt tcaccgtggc tggcgacgca tgatcccaac
     6481 gccattgatt taaccgcccg ccttttgccg ccttctgcgg cgcactggtt tggcaccgat
     6541 gaagtgggac gcgatctgtt tagccgcgta ctggtcggca gtcagcaatc aattctcgcc
     6601 ggattagtgg tggtcgccat tgcaggtatg atggggtcgc tactcgggtg tctatccggt
     6661 gtgcttggcg gacgcgcaga cgcgattatc atgcgcatca tggacattat gctgtcgatt
     6721 ccttcgctgg tgctgacaat ggcactggca gccgctctcg ggccgagttt gtttaacgcc
     6781 atgctggcga ttgctattgt gcgaattccc ttttatgtgc gcctggcgcg ggggcaaaca
     6841 ttagttgttc gccagtatac ctatgttcag gcggcgaaaa cctttggtgc gtcgcgttgg
     6901 catctgatca actggcatat tttacgtaac tccctgccgc cgctgattgt gcaggcatcg
     6961 ctggatatcg gtagcgcgat tttaatggcc gccacgttgg gatttattgg cctgggtgct
     7021 caacaaccga gtgctgaatg gggcgcgatg gtagcgaatg gtcgcaacta tgtactcgat
     7081 cagtggtggt attgcgcatt tccgggggca gcgattttgc ttaccgccgt cgggtttaat
     7141 ctctttggtg atggtattcg cgatctgctt gacccgaaag caggaggaaa gcagtcatga
     7201 cccaacccgt tctggacatt caacagctgc atttgagttt ccccggtttt aacggtgatg
     7261 ttcacgcgct caacaatgtg tccttgcaga ttaaccgcgg tgaaattgtc ggtctggtgg
     7321 gagaatccgg ctcaggtaaa tcagtcaccg caatgctgat tatgcgtctg ctaccgacgg
     7381 gcagttattg cgtacatcgg gggcagattt cactgctggg agacgatgtt cttaacgctc
     7441 gggaaaaaca gcttcgtcag tggcgcggcg cacgagtggc gatgatcttt caggaaccga
     7501 tgaccgccct taatccgaca cgtcgaatag gtcttcagat gatggacgtg atccgccatc
     7561 atcaaccaat aagtcgtcgg gaagccagag ctaaagcgat tgccctgctg gaagagatgc
     7621 aaatcccgga tgccgtggaa gttatgtcgc gctatccgtt tgagctttca ggtggtatgc
     7681 gccagcgggt aatgattgcg ctggcattct cctgcgagcc gcaattgatt attgccgacg
     7741 aaccgactac ggcgctggac gtcacggtac aattgcaggt actgcgtctg cttaaacata
     7801 aagcccgcgc cagtggaact gcggtactgt tcatcagcca tgatatggcc gtggtgtcgc
     7861 aactgtgcga tagcgtttac gtgatgtatg ccggaagcgt aattgaaagc ggcgtgacgg
     7921 cagacgttat ccatcatccc cggcatccgt ataccattgg tttgctgcaa tgcgcaccag
     7981 aacatggagt accacgccag ccattacccg ccattccagg gacggtacca aacctcaccc
     8041 atttgcctga cggctgcgct tttcgcgatc gttgctatgc ggcaggtgca cagtgtgaaa
     8101 acgtcccggc gctaacagcg tgtggtgaca acaaccaccg ctgcgcctgt tggtatcctc
     8161 agcaggaggt cattagtgtc tgacacgtta ttaacgttac gcgacgtcca tatcaatttc
     8221 cctgcccgaa aaaactggct tggtaaaact acggaacatg ttcatgccat caatggtatt
     8281 gatttacaga tccgtcgtgg tgaaacctta gggatcgtcg gtgagtcagg ctgcggcaaa
     8341 agcaccctcg cacagctttt aatgggtatg ctgcaaccta gccacgggca gtacatccgt
     8401 tcaggctcac aacgcattat gcagatggtg tttcaggacc cgctctcttc gctcaatccg
     8461 cgcttaccgg tgtggcgcat catcacagaa ccgctctgga tagctaagcg tagtagtgaa
     8521 caacagcggc gagcgttggc agaggagctg gctgtgcagg tgggtattcg tccggagtat
     8581 ctcgaccgct tgcctcatgc gttctcaggc gggcagcggc aacgtatcgc cattgccaga
     8641 gcactctctt cgcagcctga cgtgattgtg cttgatgaac caacctcggc gctggatatc
     8701 tccgtgcagg cgcagatcct caatttactg gtaacgctac aggaaaatcg cgagctgact
     8761 tatgtgctga tttcacacaa tgtttcggtg atacgtcata tgagcgatcg ggtggcggtg
     8821 atgtatctcg gacagattgt ggaactagga gacgtgcaac aggtgctgac agctccggca
     8881 catccataca cccgattatt gctggattcc ctccccgcca ttaataaacc gctggaggaa
     8941 gaatgggcat tacgtaaaac ggatctacca ggaaaccgca cgttgccaca aggctgtttt
     9001 ttccgcgaac gctgccctct ggcaacccac ggatgtgaag tccggcaatc attaacaata
     9061 agggaggacg gacgtgagat ccggtgctgg cgggcgctgt agagacgcta acaacgcatc
     9121 aggcaatcac acgcctgacg cgtcatccgg caacgcttta cgatttcaac tggtaatcaa
     9181 gcgtaatttc agctttcagt acctgagaaa ccgggcatcc tgcttttgct ttctggataa
     9241 tgccgtcaaa agtagaggca tcaatacccg gcaccgcaac ttcgctcttc agtgcgattt
     9301 tagtaatcgc aaaacctgca tccactttat ccagcgacac atcggcggtg gtatcaatcg
     9361 atgttggcgt gaatcccgct tcccccagca ttaatgaaag cgccattgag aaacatgcgg
     9421 catgcgctgc gccaatcagt tcttcagggt tggttccttt ttcgccttca aaacgcgtgt
     9481 taaatccata cggctgttgg ttcagcacgc cgctctcggt ggatacagtt cccttcccgc
     9541 gtttgatatc gccttcccag tgtgcctgac ctttcttatg gattgtcatt gttgctctcc
     9601 tgtgggctta aaaaggaaat tatagactac gtgagaaacc gcacgaggat aagcaggata
     9661 ttccgaataa aatccggcga aaacattatc tctgtactta aattaaattc ccacaataga
     9721 taacggggaa tatataatcc aaaagatatt gtatcaatat taatcggatc gccccaatta
     9781 caatccctat gaatatcatg gtgttataat ataaaaatta tcaaccagga ctaatcttaa
     9841 caacgaagcg gcaaatattt gccatgctga atgtgcttta taaatctgcg atccgtagca
     9901 gacaccaaaa atacacagac acggagaatc gctatgttta cttattatca ggcagaaaat
     9961 tcaacagcag aacccgctct ggttaatgcc attgagcaag gccttcgggc agagcacggt
    10021 gttgtcactg aagatgacat tctcatggag ctgaccaaat gggtggaagc ttctgataac
    10081 gacatcctca gtgatatcta ccagcaaacg atcaattatg tggtcagtgg ccagcaccct
    10141 acgctttaag gtgctatgct tgatcggcaa cctaatttag gggtttagca cgtgtttctt
    10201 cgctacggcg atgttgtcct taaaactagc aacaggattg aggagttaaa atgaaatcga
    10261 accgtcaggc acgtcatatt cttggactgg accataaaat ttctaaccag cgcaaaatag
    10321 ttaccgaagg tgacaaatcc agcgtggtaa ataacccaac cggcagaaaa cgccccgctg
    10381 aaaagtaatt cataaccatc agtcctcaat gacgattaaa caccattgcc tgcgcaatgg
    10441 tgtttttgtt tttatctgct ttatacttgg ggccgacgcc ctggcggtaa agcaaagacg
    10501 ataaaagcgt gccagggttg gatattcaaa aaagagtgag tgacatggaa ccaaaaacaa
    10561 aaaaacagcg ttcgctttat attccttacg ctggccctgt actgctggaa tttccgttgt
    10621 tgaataaagg cagtgccttc agcatggaag aacgccgtaa cttcaacctg ctggggttac
    10681 tgccggaagt ggtcgaaacc atcgaagagc aagcggaacg agcatggatc cagtatcagg
    10741 gattcaaaac cgaaatcgac aaacacatct acctgcgtaa catccaggac actaacgaaa
    10801 ccctcttcta ccgtctggta aacaatcatc ttgatgagat gatgcctgtt atttataccc
    10861 caaccgttgg cgcagcctgt gagcgttttt ctgagatcta ccgccgttca cgcggcgtgt
    10921 ttatctctta ccagaaccgc cacaatatgg acgatattct gcaaaacgtg ccgaaccata
    10981 atattaaagt gattgtggtg actgacggcg aacgtattct ggggcttggt gaccagggca
    11041 tcggcgggat gggcattccg atcggtaaac tgtcgctcta taccgcttgt ggcggcatca
    11101 gcccagcgta tacccttccg gtggtgctgg atgtcgggac gaataaccaa cagctgctta
    11161 acgatccgct gtatatgggc tggcgtaatc cgcgtatcac tgacgacgaa tactatgaat
    11221 tcgttgatga atttatccag gctgtgaaac aacgctggcc ggacgtactg ttgcagtttg
    11281 aagacttcgc acaaaaaaat gcgatgccgt tacttaaccg ctatcgcaat gaaatttgtt
    11341 cttttaacga tgacattcag ggcaccgcgg cggtaacggt cggcacactg atcgcagcca
    11401 gccgcgcggc aggtggtcag ttaagcgaga aaaaaatcgt cttccttggc gcaggttcag
    11461 cgggatgcgg cattgccgaa atgatcattg cccagactca gcgcgaagga ttaagcgagg
    11521 aaacggcgcg gcagaaagtc tttatggtcg atcgctttgg cctgctgacc gacaagatgc
    11581 cgaacctgct gcctttccag accaaactgg tgcagaaacg cgaaaacctc agtgactggg
    11641 ataccgacag cgatgtgctg tcactgctgg atgtggtgcg taatgtaaaa ccagatattc
    11701 tgattggcgt ctcaggacag accgggctgt ttacggaaga gatcatccgt gagatgcata
    11761 aacactgtcc gcgtccgatc gtgatgccgt tgtctaaccc gacttcacgc gtggaagcca
    11821 caccgcagga cattatcgcc tggactgaag gtaacgcgct ggtcgccact ggcagtccgt
    11881 ttaacccagt ggtatggaaa gataaaatct accctatcgc ccagtgtaac aacgccttta
    11941 ttttcccggg catcgggctg ggcgttattg cttccggcgc gtcacgtatc accgatgaga
    12001 tgctgatgtc ggcaagtgaa acgcttgctc agtattcgcc actggtgctg aacggcgaag
    12061 gtctggtact gccggaactg aaagatattc agaaagtctc ccgcgcaatt gcctttgcgg
    12121 ttggcaaaat ggcgcagcag caaggcgtgg cggtgaaaac ctccgccgaa gctctgcaac
    12181 aggccattga tgataatttc tggcaagccg aataccgcga ctaccgccgt acctccatct
    12241 aagcctgcgc ccggtagtca aggctaccgg gcaatttccc tctccctttt tcagatctca
    12301 tccatactgg gtagtggcga ataaatctca tttgcctcac ctgctatgca gaacatcatc
    12361 cgaaaaggag gaactatgaa ggctgcagtt gttacgaagg atcatcatgt tgacgttacg
    12421 gataaaacac tgcgctcact gaaacatggc gaagccctgc tgaaaatgga gtgttgtggt
    12481 gtatgtcata ccgatcttca tgtgaagaat ggcgattttg gtgacaaaac cggcgtaatt
    12541 ctgggccatg aaggcatcgg tgtggtggca gaagttggcc caggtgtcac ctcattaaaa
    12601 ccaggcgatc gtgccagcgt ggcgtggttt tacgaaggat gcggtcattg cgaatactgt
    12661 aacagtggta acgaaacgct ctgccgttca gttaaaaatg ccggatacag cgttgatgga
    12721 gggatggcgg aagagtgcat cgtggtcgcc gattacgcgg taaaagtgcc agatggtctg
    12781 gactcggcgg cggccagcag cattacctgt gcgggggtca ccacctacaa agccgttaag
    12841 ctgtcaaaaa ttcgtccagg gcagtggatt gctatctacg gtcttggcgg tctgggtaac
    12901 ctcgccctgc aatacgcgaa gaatgtcttt aacgccaaag tgatcgccat tgatgtcaat
    12961 gatgagcagt taaaactggc aaccgaaatg ggcgcagatt tagcgattaa ctcacgcagc
    13021 gaagacgccg ccaaaattgt gcaggagaaa actagtggcg ctcacgctgc ggtggtaaca
    13081 gcggtagcta aagctgcgtt taactcggca gttgatgctg tccgtgcagg tgggcgtgtt
    13141 gtggcagtcg gtctgccgcc ggagtctatg agcctggata tcccacgcct tgtgctggat
    13201 ggcattgaag tggtcggttc tctggtcggc acgcgccagg atctaaccga agccttccag
    13261 tttgccgccg aaggcaaagt ggtgccgaaa gtcgccctgc gtccgttagc ggacatcaac
    13321 accatcttta ccgagatgga agaaggcaaa atccgtggcc gtatggtgat tgatttccgc
    13381 cgctaagagg cctttgctgc gactgccatg ttcgggtcgc agcatcgcac actctccaac
    13441 atgaaatggc taaatggatt attgttgcgt aggaaggaac gctactgaga gtaaagacac
    13501 taatgaaaat ggccaatcat ccccgcccgg gggacattat tcaggaatca ctggacgaac
    13561 ttaatgtcag cctgcgcgag tttgccagag caatggaaat tgcgccctca acggcaagcc
    13621 gattgctgac cggaaaagca gctttgacac cagaaatggc gattaaactt tccgtggtga
    13681 tcggcagttc gccgcaaatg tggctgaatc tgcaaaacgc ctggagtctg gcagaggcag
    13741 aaaaaacggt ggatgtgtcg cgattacgcc gtctggttac gcaa
//
LOCUS       DACBVI010000118        13670 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.118, whole genome shotgun sequence.
ACCESSION   DACBVI010000118 DACBVI010000000
VERSION     DACBVI010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.118"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(176..628)
                     /locus_tag="IU325_16875"
     CDS             complement(176..628)
                     /locus_tag="IU325_16875"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP5810674.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFYMRYKPQH"
     gene            complement(638..1036)
                     /locus_tag="IU325_16880"
     CDS             complement(638..1036)
                     /locus_tag="IU325_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafO family
                     toxin"
                     /protein_id="HAP5810675.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(1039..1332)
                     /gene="yafN"
                     /locus_tag="IU325_16885"
     CDS             complement(1039..1332)
                     /gene="yafN"
                     /locus_tag="IU325_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HAP5810676.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRAEQYLNDMTDDDFNDFKE"
     gene            complement(1384..2439)
                     /gene="dinB"
                     /locus_tag="IU325_16890"
     CDS             complement(1384..2439)
                     /gene="dinB"
                     /locus_tag="IU325_16890"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP5810677.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPTFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVTLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2510..3295)
                     /gene="lafU"
                     /locus_tag="IU325_16895"
     CDS             complement(2510..3295)
                     /gene="lafU"
                     /locus_tag="IU325_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HAP5810678.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISLSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3240..4979
                     /gene="flhA"
                     /locus_tag="IU325_16900"
     CDS             3240..4979
                     /gene="flhA"
                     /locus_tag="IU325_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhA"
                     /protein_id="HAP5810679.1"
                     /translation="MLSRSDLLTLLTINFIVVTKGAERISEVSARFTLDAMPGKQMAI
                     DADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGGVC
                     IGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDVRN
                     QLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEKSL
                     ETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQVIS
                     DGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDGVL
                     GNDPAYGMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDITQ
                     LHNRLSSMAPRLAEDLSAALNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSTVTKD
                     HILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNVVNQAQQGGKVMLDSVP
                     VDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYNEV
                     PDELELKIMGALS"
     gene            5084..5362
                     /locus_tag="IU325_16905"
     CDS             5084..5362
                     /locus_tag="IU325_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, HicA family"
                     /protein_id="HAP5810680.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            5355..5711
                     /locus_tag="IU325_16910"
     CDS             5355..5711
                     /locus_tag="IU325_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HAP5810681.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(5768..6526)
                     /locus_tag="IU325_16915"
     CDS             complement(5768..6526)
                     /locus_tag="IU325_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012599877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan endopeptidase"
                     /protein_id="HAP5810682.1"
                     /translation="MSFMSSFLLGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQR
                     MQNRARLLKQYQTHLKKQASYIVEGNAESRKALRQHNREQIKQHPEWFPAPLKASDRR
                     WQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNK
                     ILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIES
                     PRTGETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            6818..7558
                     /locus_tag="IU325_16920"
     CDS             6818..7558
                     /locus_tag="IU325_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP5810683.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(7529..8296)
                     /locus_tag="IU325_16925"
     CDS             complement(7529..8296)
                     /locus_tag="IU325_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HAP5810684.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(8401..8979)
                     /gene="lpcA"
                     /locus_tag="IU325_16930"
     CDS             complement(8401..8979)
                     /gene="lpcA"
                     /locus_tag="IU325_16930"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HAP5810685.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            9219..11663
                     /locus_tag="IU325_16935"
     CDS             9219..11663
                     /locus_tag="IU325_16935"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAP5810686.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKNHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYKAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPDKVRKVEAA"
     gene            complement(11706..12179)
                     /locus_tag="IU325_16940"
     CDS             complement(11706..12179)
                     /locus_tag="IU325_16940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-lysozyme inhibitor"
                     /protein_id="HAP5810687.1"
                     /translation="MGRISSGGMMFKAITTVAALVIATSAMAQDDLTISSLAKGETTK
                     AAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEK
                     SNQMTGLFSTIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            12333..13103
                     /gene="yafV"
                     /locus_tag="IU325_16945"
     CDS             12333..13103
                     /gene="yafV"
                     /locus_tag="IU325_16945"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HAP5810688.1"
                     /translation="MSGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            complement(13198..13509)
                     /locus_tag="IU325_16950"
                     /pseudo
     CDS             complement(13198..13509)
                     /locus_tag="IU325_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(13528..>13670)
                     /locus_tag="IU325_16955"
     CDS             complement(13528..>13670)
                     /locus_tag="IU325_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HAP5810689.1"
                     /translation="FSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSG
                     LS"
BASE COUNT         3253 a         3487 c         3582 g         3348 t
ORIGIN      
        1 aatataaaaa tcaggaaatg gcagacgaat ccagggcaag aaaaaaagaa ttaatcctgg
       61 aaaggtctgc aaagagtcat tgataaagga tttgtcatga tttacctcct tgcagtttgt
      121 ggaataagaa aacgcgcaca tactaggcgg gcgttatttt cattgcaagc tggatttaat
      181 gttgcggttt atatcgcata taaaaattag taaaccacgc tccccggcat tcaacgcgct
      241 gctgcttaac tgtctgaaaa ccataacgtt caaaaaaggg ttttgcggtt atgctggcat
      301 ctaccgttag ttcggattca gacttaatca aaggttttaa caaagcgctg gcaaccccac
      361 ggcgggtgta ttcagggtca acaaataaca tatcgatata atgttcaacg caggtaataa
      421 agccgaccag ttttgcatta atgactgcaa cccgcacttg tgatttcgcg agtttctcct
      481 tccagcgaga ttcgtcaatc tgcgcccagg cggcaatttg ttgtggtgaa taatgctgac
      541 tggcggtcat cgtaaccgct ctaatgaaaa tagcgcatag ttgctgaaaa tcgccaggct
      601 gataatctct tatttgtatg ttattcataa tataaattca aaaacgcatg cgaaacgctt
      661 ctgccatttt tccaatttta tgcatttggt tgttatctcg agccagttta tgaggttcag
      721 gcttcagaat ggcaatgagc aaccatgctt gctcatcaaa cgcaccctga caatatacca
      781 aatgcgcttc gtcattcgtt ctgctgaatt ggcgcaactg tggcggaaat ggattattca
      841 catttgccag atgaatatga gcaactcgct caaatttgat taatggccag gtaaacgagt
      901 cgtcgtagag tgcatcgcga ccaaatatat ctggcaaaac accgtcacgc ttataggaaa
      961 taaaatccgc cgttaacgca tcaagttcct ctgctgtaag ttgcaggcga ataagttttg
     1021 ttttgaatac ccgcatcctt attccttaaa gtcattgaaa tcatcatccg tcatatcatt
     1081 aagatattgt tcagcgcggc gtgtaatttc ctctaatctt gcggcactcg gacggaagcg
     1141 cgccttcacc agttttttct cctcttgttc tgcaagcatg tccattaacg cttcgaatgc
     1201 gctggcactt aagagatatc ctgcggggcg attattagaa agaaccgcaa ccggttgatc
     1261 aataaagtat tttgctggat ttttacgtaa ctcagtgata ttgaccgatt tttcagcgag
     1321 aattcgatgc atacagtgat accctcataa taatgcacac cagaatatac ataatagtat
     1381 acatcataat cccagcacca gttgtctttc catttgcggg tcaagcaacg tcacatgcag
     1441 ccccaccagc cgcacaccgc gcccgccgcg acgttcatcc caggttttac gcgcggtggc
     1501 aattaagtca gctttattca gccgcggcca gacgtgctcc tgagtggttt gctgaaaatc
     1561 atcaaacttt aatttcaccc cctggcgagc aatcagtaaa tcaggtttta cttttgccag
     1621 acggcgttca agttccgggt acagccgctc gataatcgct tcacattcag accagtgatg
     1681 aatatcttcc gccatcgtgc gttccacgcc gacggatttt cgcaaccgtt cgctgttaac
     1741 gtcgcgctcg tcaatcccct gactacgctc ccacaaaatg cggccaaatt tgccaaaacg
     1801 tttgagcaga gtcaccagat cacacttttg cacatcaccg caggtccgca gccccatcgc
     1861 ttccagtttt gccgccgaga ctttgccgac gccggggatt tttgccagcg gtaaggtttg
     1921 taaaaatgtc ggaacttctg ccggcgtaat cacaaactgg ccgttgggtt tattcatgtc
     1981 ggaggcgatt ttggcgagaa actttactgg tgccacgccc gcagacgccg tcagttgcag
     2041 ctcgctgaaa atcgtctggc ggatttcctg ggcgatgagg gtcgcagaac cgtggcaatg
     2101 gacgctatcg gtgacatcga gataggcctc atccagtgac aacggttcaa tgcgcgaggt
     2161 gtagcgcgag aagatttcac ggatatgatt tgaggcttct ttgtaggcgt caaagcgccc
     2221 cggaagcaag gtgagatgtg ggcataattt gagcgccatc ccagtaggca tagcgctacg
     2281 tacgccaaat ttacgcgcgg gataattggc ggtgctgatc accccccgac gttcgcggct
     2341 gccgccaata gcaataggga tatcgcgcag ggcgggattg tcgcgcatct ccactgcggc
     2401 gaaaaagcag tccatatcca catgaatgat tttacgcatt gctcacctct caacactggt
     2461 aaagtataca gtgatttcag ggtttgagaa atgcgtaaag attcagcatc tagcgcgttc
     2521 gctgcgaaag cacctgctgc ttatccagct tttgcaccag cggctgcacc actttatccc
     2581 catgctgacc aaagtattga tacagcgtat cggacgcact tttggtcagc accataatct
     2641 caatgcgtcg gttgcccgcg ctttgcggat ttttcgcgtc cagcagcatc tggtccgcca
     2701 tcgcgctcac ctgcatcact ttatcttccg gcattccggc ctcttccagc acccgacgag
     2761 ccgaaagcgc gcgatcgccc gaaaggttcc agttgttgta gatattgttt ttgtaggcca
     2821 tcgcatcggt atgcccggta ataataattt tgttatcgag cgagtcgaac actggcgcga
     2881 gctccaccag caaagtttta aagaacggca taatctgtgc gctgccgcgt tcaaacatat
     2941 tgcggttctg gtcatcttta atcagcacgc gcagtccctg gggaacaatc tccatctcca
     3001 gattcgcttc catatgtgca tcacgggcaa tggtgttgat actggtcgcc agctcgccaa
     3061 gctcagtagc ggatttcttc ttcagcagcg ccgttttctc attgacgtca cgcgcttttt
     3121 tctccgtctc ttccggcacc gccacggtgg caggttttgg cggatgagac aggctgattt
     3181 tatttaacgg cgacaacccg ccgccattga aaatcgactg cccgtgcagc gcggcgataa
     3241 tgctttcacg ctcggattta ctgacgctgt tgacaatcaa ctttatcgtc gtcaccaaag
     3301 gggccgagcg tatttccgag gtttctgccc gctttaccct ggacgcgatg cccggcaaac
     3361 agatggcgat tgacgccgat cttaacgccg ggttgatcaa ccaggcgcag gcgcaaaccc
     3421 ggcgtaaaga tgttgccagc gaggccgatt tctacggcgc aatggacggg gcatcgaagt
     3481 ttgtgcgcgg ggatgccatc gccgggatga tgattctggc gatcaacctg atcggcggcg
     3541 tctgtatcgg gatcttcaaa tacaacctga gcgccgatgc cgccttccag caatatgtgc
     3601 tgatgaccat cggcgacggc ctggtggcgc agatcccgtc cctgctgctt tccaccgcgg
     3661 cggcgattat cgtcacccgc gtcagcgaca acggcgatat tgcccatgac gtgcgtaacc
     3721 agttgctggc aagcccgtcg gtgctctaca ccgccaccgg gatcatgttt gtgctggcgg
     3781 ttgtgccggg gatgccgcat ctgccgtttt tgctgttcag cgccctgctt ggttttaccg
     3841 gctggcggat gagcaaacgc ccgcaggcgg cggaggcgga ggagaaaagc ctcgaaacgc
     3901 tgacccgcac catcaccgaa accagcgagc aacaggtcag ttgggagact attccgctga
     3961 tcgaacccat cagtttaagc ctcggttaca aactggtggc gctggtcgat aaagcgcagg
     4021 gtaacccact cacccagcgt attcgcggcg tgcggcaagt gatatccgac ggcaacggcg
     4081 tgctgctgcc ggagatccgt attcgggaaa acttccgcct caagcccagc cagtacgcca
     4141 tattcatcaa cggcattaag gccgatgaag cggatattcc ggcggataaa ctgatggccc
     4201 tgccctccag cgaaacctac ggcgagattg acggcgtgct ggggaacgac ccggcgtacg
     4261 ggatgccggt gacctggatc cagcccgcgc agaaagcgaa agcgctgaat atggggtatc
     4321 aggtgatcga cagcgccagc gtgatcgcta cccatgtgaa caagattgtg cgcagctata
     4381 ttcctgattt gtttaactat gatgacatca cgcagttgca taaccgtttg tcgtcgatgg
     4441 caccgcgcct ggcggaagat ttgagcgcgg cgctcaatta cagccaattg ctgaaagtgt
     4501 accgggcgct gctgaccgaa ggcgtttccc tgcgcgatat tgtcaccatc gccaccgtgc
     4561 tggtcgccag tagcacagtg accaaagatc atatcctgct ggcggccgat gtgcgcctgg
     4621 cattgcggcg cagcattacc catccgttcg ttcgcaagca ggagctgacg gtgtatacgc
     4681 tgaataatga gctggaaaat ctgctcacca acgtggtgaa tcaggcgcaa cagggcggga
     4741 aagtgatgct cgacagcgtg ccggtcgatc cgaatatgct caaccagttc cagagtacga
     4801 tgccgcaggt aaaagagcag atgaaagcgg cggggaaaga cccggtgctg ctggtgccgc
     4861 cgcagttgcg tcctttgctg gcgcgttatg caaggttgtt tgcgccgggg ctgcatgtgc
     4921 tgtcgtataa cgaagtgccg gacgagctgg agttgaagat tatgggggcg ttgagttgat
     4981 tgaggaatct tttccatgct ttgcagcacc tgacaccatt aattgacgtc acactgcaaa
     5041 gagactacga tgacaccata caatgacgtc atggattgat agtatgatta aggtcagcgc
     5101 gctaggctct cgtcatagaa aaacattcga cgatgtaatg acggagccgc caaaatctgg
     5161 aattaaatgg gatgatgttg ttgcactcat taaagctgtg ggtggaacaa taaaaaataa
     5221 caacggctcc agacgaaaat ttcagattgg aacgaccaaa ttcagcacac atgagccaca
     5281 tccaaagaat gtgatggata aaggcgcagt agcaggtctt aaggagtggt ttgttaactg
     5341 tgtaggggta gattatgaat aaagcatcga acattatcac aattaaaggt caaccagccg
     5401 cagtgacatt tgaagccgat atcaacgcat ttcgcggcaa gttcctcaat gtaaacggtt
     5461 attgtgactt tgtagccaca agtattgagg cattatatcg tgaaggcgaa agcgcgctgg
     5521 atgagtggat ggcggattgt gaagaggacg gtattgcccc ttttcgtgaa gaagaagagc
     5581 aaaaacgcct gacgcttcgt gttcctcatc acattgattc gcggctgacc atcgtcgcca
     5641 gacaacattc catttcaaaa aaccagctca ttgttgatgt cttagaacgt gagttttcag
     5701 cccagatgta aacaggtcgg atgccgcgtg gcatccgacc ttttgccagt tcgtcaatat
     5761 tacccattta caaaatcgtc tcttccgtca aaatccggcg cgcgcctaaa aaatggtcct
     5821 gccagaaagg ttcagctaac cgacttaccc gtatggtttc cccggtgcgc ggcgactcaa
     5881 taaactgtcc atcgcccaga tacacgccca tatggtcggc aatctcgcgg ctgtggatat
     5941 gaaaaaacag caaatctccc cggcgcaggt cgttattcgc cacaatggtt gcccggcgat
     6001 agtgatacat ctcattagcc gtgcgtggga gcttcgcctc gaggatcttg ttgtaggcat
     6061 aaaaaaccaa cccgctacag tcgaagcctt gatcgggccg cgtgccgccc cagacgtaag
     6121 gcttgccaag ctgctgctcc aggcggtgaa tcgccacttc ggtgatgttg tgtagatggt
     6181 cgctgcttaa gaaatggttg ttctccgcca gcgcctgcca gcgtctgtca cttgccttga
     6241 gcggcgcagg aaaccattct ggatgctgtt ttatctgttc gcggttgtgc tggcgcagcg
     6301 cctttctgct ttcggcattg ccttccacaa tatagctggc ctgttttttc agatgggttt
     6361 ggtactgttt taataaacgc gcacggtttt gcatccgctg gcgggcggcg tagctgaaag
     6421 agatatgtga tgtggtggcg ctggcgagta aagggagcaa aacgcataac gaaaacacgc
     6481 ctggatgcag aaatctgccg agcaagaacg aagacatgaa ggacataaac cggctgaatg
     6541 aaggtgagaa ttttaaaaga ccagaggagt aaacgggatt attaattgct tatttgcata
     6601 ttactattga ggtttcataa tcttaggaaa taaatgatac atgcgctttt cggactattg
     6661 aaaactgccc ctgtgctaaa tttgtaaacc ctgcgccgac aggcttgcgt aaaactcttt
     6721 tggtgataat gtgagccttc ccaatccaga ctattccgat ttgggatcca ttttgccgtc
     6781 ctggcgtgtg taaccgtttt atcaaggaat aagcagtatg cgtaaaatcg cattaattct
     6841 tgcgatgttg ttgatcccgt gcgtttcgtt tgccggtttg ctgggcagca gtagctcgac
     6901 cacgccagtg agcaaagagt ataagcagca gttgatggga tcccctgtct acatccagat
     6961 cttcaaggaa gaacgtacac tcgatctcta cgtcaaaatg ggcgagcaat atcaactgct
     7021 cgacagctat aaaatctgta aatattccgg cggcttaggg ccaaaacagc gtcagggcga
     7081 tttcaaaagc ccggaagggt tttatagcgt ccagcgtaat cagttaaaac cagacagccg
     7141 ttactacaaa gcgattaata ttggattccc caatgcctat gaccgtgcgc atggttatga
     7201 agggaaatat ctgatgatcc acggcgattg tgtttccatc ggctgctacg cgatgaccaa
     7261 tcagggtatt gatgagatct tccagttcgt tactggtgcg ctggtttttg gtcagccgag
     7321 cgtgcaagtg agtatttacc cgttccgcat gaccgacgcc aatatgaagc gccataaata
     7381 ttccaacttt aaggacttct gggagcaact gaagccgggc tacgactact ttgagcaaac
     7441 ccgtaagcca ccgaccgttt ctgtggtcaa tggccgttac gtggtcagca agccgttaag
     7501 ccacgaagtt gtgcaaccac agctggcatc aaactacacg ctccccgagg caaaataagc
     7561 gccattcgcc tggcataatc ttttgccagg tttcattgcc cgtcagcggc tgtgtcgcaa
     7621 tcaccgtgac cacatcattc ggtgtggtct gcgagctgaa gtcgatttcc acatcctgat
     7681 ccagcaacgt tgccacgcca aacggcgcgc ggcgggtgat ccagtgtaaa ttagtcgagc
     7741 aatacgccat tacatagcgc ccgtcagaaa gcagcatgtt gaaaacgccc ttctgccgca
     7801 gttcatccgc cagtgaggcg atatatttaa acactgccgc catgttgccc ggtgtgcgcg
     7861 ggtaacgctg cgttaattta tgcaggagcc agcagaaggc tttttcgctg tcggtttcac
     7921 cgaccgggcg gaagttgccg gtttccagtg atttgtatcc cgtcagttgt ccgttatggg
     7981 cataagtcca gttacgcccc cataactcgc gggtaaacgg gtgagtattt tccagcgcca
     8041 cctcgccccg attcgcctgg cgaatatgag ccaccaccga acaggatttt atcgggtagt
     8101 cctggacaag tttggcgatg ggggaattaa agctgggttg cggatcttta aatgtgcgac
     8161 agcctttacc ttcgtaaaag gtaatgcccc agccatcttt atgtggcccg gttccaccac
     8221 cacgctgtac aagtccggtg aaactaaagc agatatcggt agggacgttg gcgctcatcc
     8281 cgagcaattc gcacataggt aacccccaca acagcgggag cgcatggccc ccggcgaaga
     8341 ttgatttaag tattttcagg tcggatgcgg cgtaaacgca ttattcggcc tacgccagac
     8401 ttacttaacc atctcttttt caattaactg gatcaggata tggatcactt taatgtggat
     8461 ctcctgaatg cggtcggcat aaccaaagtg cggtacgcga atttcgatat ccgctgtgcc
     8521 agccatttta ccgccgtctt taccggtcag ggtgatcact ttcatcccct tctcacgcgc
     8581 cgccgcgatc gctttgatca cgtttgcaga gttaccggag gtggagatcc ccagtagtac
     8641 atcgccttcg cgacctaccg cttcaacgta acgggagaaa atatcgttga aaccaaaatc
     8701 gttaccgacg caggaaatat gactaacgtc agaaatggca atcgccggat agcccggacg
     8761 gttttcacgg tagcgccccg tcaactcttc ggcaaagtgc atagcgtcgc aatgggagcc
     8821 gccgttgcca caggaaagca ctttgccacc agctttaaaa ctgtctgcta acaggaccgc
     8881 cgcgcgctga atggcgtgaa tattggcgtc atcttttaaa aagttagcca gcgtttccgc
     8941 cgcttcgttc agttcgttac gaataagatc ctggtacatg aggatatcct tcagcataaa
     9001 tgtaatagac aaaatgcagt gtaccggata ccgccaaaag cgagaagtac gggcaggtgc
     9061 tatgaccagg actttttgac ctgaagtgcg gataaaaaca gcaacaatgt gagctttgtt
     9121 gtaattatat tgtaaacaca ttgctaaata tttttacatc cactacaacc atatcatcac
     9181 aagtggtcag acctcctaca agtaaggggc ttttcgttat gatgattttg agtattctcg
     9241 ctacggttgt cctgctcggc gcgttgttct atcaccgcgt gagcttattt atcagcagtc
     9301 tgattttgct cgcctggaca gccgccctcg gcgttgctgg tctgtggtcg gcgtgggtac
     9361 tggtgccgct ggccattatc ctcgtgccgt ttaactttgc acctatgcgt aagtcgatga
     9421 tttccgcgcc ggtatttcgc ggtttccgta aggtgatgcc gccgatgtcg cgcactgaga
     9481 aagaagcgat tgatgcgggc accacctggt gggagggcga cttgttccag ggcaagccgg
     9541 actggaaaaa gctgcataac tatccgcagc cgcgcctgac cgccgaagag caagcgtttc
     9601 tcgacggccc ggtagaagaa gcctgccgga tggcgaatga tttccagatc acccatgagc
     9661 tggcggatct gccgccggag ttgtgggcgt accttaaaga gcatcgtttc ttcgcgatga
     9721 ttatcaaaaa agagtacggc gggctggagt tctcggctta tgcccagtct cgcgtgctgc
     9781 aaaaactctc cggcgtgagc gggatcctgg cgattaccgt cggcgtgcca aactcattag
     9841 gcccgggcga gctgctgcaa cattacggca ctgacgagca gaaaaatcac tatctgccgc
     9901 gtctggcgcg tggtcaggag atcccttgct ttgcactgac aagccccgaa gcgggttccg
     9961 atgcgggcgc gattccggac accgggattg tctgcatggg cgaatggcag ggccagcagg
    10021 tgctgggcat gcgtctgacc tggaacaagc gttacattac gctggcaccg attgcgactg
    10081 tgcttggcct ggcgtttaaa ctctccgacc cggaaaaact gctgggtggc gcagaagatt
    10141 taggtattac ctgtgcgctg atcccgacca ccacgccggg cgtggaaatt ggtcgtcgcc
    10201 atttcccgct gaacgtaccg ttccagaacg gaccgacgcg cggtaaagat gtcttcgtac
    10261 cgatcgatta cattatcggc ggaccaaaaa tggccgggca aggctggcgg atgctggtgg
    10321 agtgcctctc ggtaggccgc ggcatcaccc tgccttccaa ctcaaccggc ggtgtgaaat
    10381 cggtagcgct ggcaacgggc gcgtatgcgc acattcgccg tcagttcaaa atttctattg
    10441 gtaagatgga agggattgaa gagccgctgg cgcgtattgc cggtaatgcc tatgtgatgg
    10501 atgctgcggc atcgctgatt acctacggca ttatgctcgg tgaaaaacct gccgtgctgt
    10561 cggctatcgt taagtatcac tgtacccacc gcgggcagca gtcgattatt gatgcgatgg
    10621 atattaccgg cggtaaaggc attatgctcg ggcaaagcaa cttcctggcg cgtgcttacc
    10681 agggcgcacc gattgccatc accgttgaag gggctaacat tctgacccgc agcatgatga
    10741 tcttcggaca aggagcgatt cgttgccatc cgtacgtgct ggaagagatg gaagcggcga
    10801 agaacaatga cgttaacgcg ttcgataaac tgctgttcaa acatatcggt cacgtcggca
    10861 gtaacaaagt tcgcagcttc tggctgggcc tgacgcgcgg tttaaccagc agcacgccaa
    10921 ccggcgatgc cactaaacgc tactatcagc acctgaaccg cctgagcgcc aacctcgccc
    10981 tgctttctga tgtctcgatg gctgtgctgg gcggcagcct gaaacgtcgt gagcgtatct
    11041 cggcccgcct gggggatatt ttaagccagc tttacctcgc ctctgccgtt ctgaagcgtt
    11101 atgacgacga aggccgtaat gaagccgatc tgccgctggt gcactggggc gtacaggatg
    11161 cgctgtataa ggctgaacag gcgatggacg atttactgca aaacttcccg aaccgcgtgg
    11221 ttgccgggct gctgaatgta gtgatcttcc cgaccggacg tcattatctg gcaccgtctg
    11281 acaagctgga tcataaagtg gcgaagattt tacaagtgcc gaacgccacc cgttcccgca
    11341 ttggtcgcgg tcagtacctg acgccgagcg agcataatcc ggttggcttg ctggaagagg
    11401 cgctggtgga tgtgattgcc gccgacccaa ttcatcagcg gatctgtaaa gagctgggta
    11461 aaaacctgcc gtttacccgt ctggatgaac tggcgcataa cgcgctggcg aaggggctga
    11521 ttgataaaga tgaagccgct attctggtga aagctgaaga aagccgtctg cgcagtatta
    11581 acgttgatga ctttgatccg gaagagctgg cgacgaagcc ggtaaagttg ccggataaag
    11641 tgcggaaagt tgaagccgcg taattgaata acggagccga aaggctccgt ttctttatcc
    11701 gctaattatt taaaattaaa gccatccgga tggttttcca ggctgccggt caacgccgcg
    11761 aacagcaccg ttttaccatc aatcgaaagc gcatcgttca cattcagcca ggtgagtttc
    11821 tcttgcgacg ttttctcatc aatagtcgag aacagccccg tcatctgatt agatttctcg
    11881 gaccacatca cagcgatacg ttgcgagcca cagtcatgcg gtttgcacgc gctcatcacc
    11941 tgatacgtct catctcccaa cgttacggtt tgtgcgggag tataagtacc gcctttcatc
    12001 acccatgcag gtagcttatg cccttgcacc atctgattaa acgcagcttt ggtggtttcg
    12061 ccctttgcaa ggctgctaat ggttaaatca tcctgcgcca ttgcactggt ggcgatgacc
    12121 agagcggcga ctgtcgttat tgccttaaac atcattcctc ccgagcttat cctgcccatg
    12181 ttattaacct cctaaagcct ggcgataaca cgataatttg caaatcagat atttccaata
    12241 tcagcatttg ttatcaattt attctgcgct aaggcaactg cctgataagg tgttagagtt
    12301 ataaaaccct tcgcataaag gagttgtaca ccgtgtctgg tttgaagatt acgcttttac
    12361 agcaaccact ggtgtggatg gatggtcctg ccaacctgcg tcattttgat cgtcaactgg
    12421 aaggtattac cggacgcgat gtgatcgttc taccggagat gtttaccagc ggctttgcca
    12481 tggaagcggc agcttcgtcg ctagcacaag atgacgtagt gaactggatg acagccaaag
    12541 cgcagcagtg caatgcgctg attgcaggca gtgttgcatt acaaacggag tctggttcgg
    12601 ttaaccgctt tttgctggtt gagccgggcg gcacggtaca tttttatgat aagcgtcatc
    12661 tgttccgcat ggcagatgag catctacatt ataaagcggg caatgcgcga gtgattgtgg
    12721 aatggcgcgg ctggcgtatt ttgccgctgg tgtgctacga cttacgtttt cctgtgtggt
    12781 cgcgcaatct caacgattat gacctcgccc tatacgtcgc caactggcct gctccgcgct
    12841 ctctgcactg gcaggcattg ctgacggccc gcgcgattga gaatcaggcg tatgtggcgg
    12901 gatgcaatcg cgtcggcagc gatggcaacg gctgccatta tcgcggtgac agccgggtga
    12961 ttaatccaca gggcgagatt atcgctactg ccgacgcgca tcaggcaacg cgcattgatg
    13021 cggagctgtc gatggtggcg ttgcgggaat atagagaaaa gtttccggca tggcgggatg
    13081 cggatgagtt taggttgtgg tgagtgggtg ttgtgtttag gggcggggta agactacggg
    13141 tcagggcaag attacgaaag cccggagcct gtccatcatt ctgtgtaaat gcctttctca
    13201 gaagtgaccg tcaaggcggt caccgaactc gataataaag cgactcatcc ctatccgcca
    13261 gtcctgaagt ggcatcgtcc atttttgcga cgctgactgg aaagccagcc acaccacttt
    13321 tttcaccgac tcatctgtcg ggaacacttt ccgtttcttt atcgcatgtg tgatgatgct
    13381 attcagcgat tcgattgcgt tagtggtgta gataaccttg cggatcgcct ccgggtacac
    13441 gaagaatgtg gcaacattcg gccagtttgc ccgccagctg cgacttatct gcgggtaccg
    13501 tgcatcccat tccttcccag ggaaagatta cgaaagcccg ctccccgcaa ggactgacgc
    13561 cagatagttt ctgtccatgg ctgcttttcg catcttacgt cttaaccctg ccttgaatac
    13621 cttatcattc gtcaaaatat taatagcgat gtgccgtatc cctgaaaata
//
LOCUS       DACBVI010000119        13584 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.119, whole genome shotgun sequence.
ACCESSION   DACBVI010000119 DACBVI010000000
VERSION     DACBVI010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.119"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            135..1625
                     /gene="ygjG"
                     /locus_tag="IU325_16960"
     CDS             135..1625
                     /gene="ygjG"
                     /locus_tag="IU325_16960"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HAP5810690.1"
                     /translation="MITEFVFIPIFAIAAGVAQSLQYLNRYHVIREPPEHILNRLPSS
                     ASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKEHVNPGFLEYRKSVTAGGDYGA
                     VEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQLAKQPLHSQELLD
                     PLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKLAKAYQSPRGKFTFIATSGAFH
                     GKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALSECKKTGDDVAAVILEP
                     IQGEGGVILPPPGYLTAVRKLCDEFDALMILDEVQTGMGRTGKMFACEHENVQPDILC
                     LAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVLLEQNL
                     PAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEMFRQRV
                     LVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALAAMRVSVEEA"
     gene            complement(1667..1999)
                     /locus_tag="IU325_16965"
     CDS             complement(1667..1999)
                     /locus_tag="IU325_16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HAP5810691.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            2218..3201
                     /gene="ebgR"
                     /locus_tag="IU325_16970"
     CDS             2218..3201
                     /gene="ebgR"
                     /locus_tag="IU325_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HAP5810692.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPSSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            3385..6477
                     /gene="ebgA"
                     /locus_tag="IU325_16975"
     CDS             3385..6477
                     /gene="ebgA"
                     /locus_tag="IU325_16975"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HAP5810693.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISTMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERLVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLCDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVIPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPISGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            6474..6923
                     /locus_tag="IU325_16980"
     CDS             6474..6923
                     /locus_tag="IU325_16980"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HAP5810694.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQVVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            6986..8419
                     /locus_tag="IU325_16985"
     CDS             6986..8419
                     /locus_tag="IU325_16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP5810695.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            8553..9623
                     /gene="ygjJ"
                     /locus_tag="IU325_16990"
     CDS             8553..9623
                     /gene="ygjJ"
                     /locus_tag="IU325_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HAP5810696.1"
                     /translation="MKLITAPCRALLALPFCYAFSAVAEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALQVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GIMASAMYEALKGVYLSTAYTYAKHRHDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQLFLMQYFYW"
     gene            9640..11991
                     /locus_tag="IU325_16995"
     CDS             9640..11991
                     /locus_tag="IU325_16995"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP5810697.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNAINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     SKKVDFTLEAYSIPGALVQKLTAKDMQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLPNGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            12283..>13584
                     /locus_tag="IU325_17000"
     CDS             12283..>13584
                     /locus_tag="IU325_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810698.1"
                     /translation="MVTPCHIKASAGMTLPLALENQESCRTRLEEMINLINRNTDCST
                     GVPDQVNALVATMADALQPYTVMTNELPEYPNMPERVLYIPEPFSFCFSGHTITFTNT
                     KRDYNGDNLVNIAVSKDGGNARGDADCALFHAFVTKMMSRSRQPGTYDFFPSLKEEKQ
                     GEGMHFMVTPRNSSISAGAIMSRPLLQTQHGCEILNKIINLINADTDEVNELVVAMTN
                     ALFLYQTVVRTELQRTEPFTEPFRFSFFFSSHTITFTKAESNSKLVQIEVSRGGVTVR
                     ECVGSARFRTIAAAMVSCRISEAAATSLALQQTLHSRVILKLMIKLINADTDRSIAVP
                     DRVNELAVAMTNALYRYQTVRGDKQIIPESFSFGFSGHTITVTPGRYSRSVNLKVCGG
                     GVPACATADRTLFRTIATALVSSNQQGTLDFRSSLPFLVERN"
BASE COUNT         3342 a         3564 c         3523 g         3155 t
ORIGIN      
        1 aattatcttg cccaaaaatc aggcagttgt tgccctgaaa gtgtgcattt gtgaagcaat
       61 cgccaaatcc atacccgaca aaaaccgtgc aaaataacaa catatgttaa caaatagcat
      121 taaatattgc acaaatgata accgaatttg tgtttatccc gattttcgcg atcgcagccg
      181 gagtggcgca atccctgcaa tacttaaatc ggtatcatgt gatacgcgag cctccggagc
      241 atattttgaa caggttacct tcgagcgcat ctgctttagc gtgcagcgcc cacgccctga
      301 atctcattga aaagcgaacg ctggatcatg aggagatgaa agcacttaac cgggaggtga
      361 ttgaatactt caaagagcat gtcaatccgg ggtttttaga gtatcgcaaa tctgttaccg
      421 ccggcgggga ttacggagcc gtagagtggc aagcgggagg tttaaatacg cttgtcgaca
      481 cccagggaca ggagtttatc gactgccttg gaggttttgg aattttcaac gtggggcacc
      541 gtaatccagt tgtggtttcc gccgtacaga atcaacttgc gaaacaaccg ctgcacagcc
      601 aggagctgct cgatccgtta cgggcgatgc tggcgaaaac ccttgcggcg ctaacgcccg
      661 gtaaactgaa atacagcttc ttctgtaata gcggcaccga gtccgttgaa gcagcgctga
      721 agctggcgaa ggcttatcag tcaccgcgcg gcaagtttac ttttattgcc accagcggcg
      781 cgttccacgg taaatcactt ggcgcgctgt cggccacggc gaaatcgacc ttccgcaaac
      841 catttatgcc gctgctgcca gggttccgtc atgtaccgtt tggcaatatc gaagccatgc
      901 gcacggccct tagcgagtgc aaaaaaaccg gtgatgatgt tgctgcagtg atccttgagc
      961 cgattcaagg tgaaggtggc gtcattctgc cgccgccggg ctatctcacc gccgtgcgta
     1021 agctgtgcga tgagttcgac gcactgatga tcctcgatga agtgcaaacc ggtatggggc
     1081 gcacgggcaa gatgttcgcc tgcgagcatg agaacgtaca gccggacatc ctgtgtctgg
     1141 caaaagccct cggcggcggc gtgatgccga ttggcgcaac catcgctact gaagaggtgt
     1201 tttccgtcct gttcgacaac ccgttcctgc ataccaccac ctttggcggc aacccgctgg
     1261 cctgtgcggc ggcgctggcg accatcaatg tgttgctgga acaaaactta ccggctcagg
     1321 ctgaacaaaa aggcgatatg ttgctggacg gtttccgtca actggcgcgg gaatatcccg
     1381 atctggtaca ggaagcgcgt ggtaaaggga tgttaatggc gattgagttt gttgataacg
     1441 aaatcggcta taactttgcc agcgagatgt tccgccagcg cgttctggtg gccggaacgc
     1501 tcaataacgc caaaacgatc cgcattgaac cgccactgac actgaccatt gaacagtgtg
     1561 aactggtgat caaagcggcg cgtaaggcgc tggcagccat gcgagtaagt gtcgaagaag
     1621 cgtaatatca aatcggatgg cgatacgacg tcgccatccg atctttttat acgatacgta
     1681 cgcccgcagg catcatccgc tccggcgtta acagcacgct ttcactgccg tcatcggttt
     1741 ccgcacacaa cagcatgcat tccgacgttt caccgcgcat tttcgctttt tgcagattgc
     1801 ataagaccac caccgttttt cccatcagct cttcttcgct gtagtacggc accaggctgg
     1861 tcacggtttg cagcgttttt tcacccacat caacctgtac gatgtacagc ttgtcggcgt
     1921 tttcatgacg tttcacttcc acaatctttc cgacgcgcat ttccagacgt gcaaaatcag
     1981 cgtaagccac ggtttccatt gtttcctccc ttcgggtaaa attttactaa actatagcaa
     2041 aagtttttct caatcctgta ggctaaaaat ggagaatgca ggcgtgatca cattcgcaag
     2101 ccgctgtgtt accgttacag cgtcaaagaa acgcgcttta tttactgaaa acaggtgatc
     2161 cgataagcac ttcctctaca atggaggcgc acatcaggga aagtaaaaaa ggtaaacatg
     2221 gcaacactaa aagacatcgc aatcgaagct ggcgtatccc tggcgacagt atccagggtc
     2281 ttaaatgacg atccgacatt gaatgtgaaa gaagagacga aacatcgcat tctcgagatc
     2341 gccgaaaagc tggagtacaa gaccagtagc gcccgtaaac tccagacagg cgcagttaac
     2401 caacaccata ttctggctat ctacagctac cagcaggagc tggagatcaa cgatccttac
     2461 tatctggcga tccgccacgg cattgaaact cagtgcgaaa agctggcgat cgaactgacc
     2521 aactgttatg aacacaacgg cttaccagac attaaaaacg tcaccggtat tttaattgtc
     2581 ggcaaaccca cgcccgccct gcgcgccgcc gcctgtgcgt tgaccgacaa tatctgtttt
     2641 atcgactttc acgaacctag cagcggttac gatgcggtgg atatcgatct ggcacgcatc
     2701 agtaaagaaa tcatcgactt ctatatcaac cagggcgtta atcgtattgg ttttattggc
     2761 ggtgaagatg agccaggcaa ggcggatatt cgtgaggtcg cctttgcgga atatggccga
     2821 ctgaaacaag tggtacgcga agaggatatc tggcgcggcg gtttttccag ttcgtcgggc
     2881 tatgaactgg caaaacagat gctggcgcgg gaagactatc cgaaggcact gtttgttgct
     2941 tccgattcca ttgctatcgg cgtactgcgg gcgattcatg aacgtggcct gaacatccca
     3001 caggatattt cacttatcag cgttaatgat atccccaccg cgcgatttac ctttccgccg
     3061 ctctccaccg tgcgcatcca ttccgaaatg atgggaagcc agggcgttaa cctggtgtat
     3121 gaaaaagccc gcgatggtcg cgcgctgccg ctgttagtct tcgttcccag caaattaaaa
     3181 ctgcgcggca cgacccgtta aatcccctta cacactgtcc ggcaatcgtt tttgccggac
     3241 agtgctgccg tttattttcg tgatccagtt aaagtaaatg catttacccg ctgcttttta
     3301 gtaaaaattt tactaaactc cccagcaatt acacaaacta ccatcaccat aaatggttcc
     3361 gatttctctc taccgggagg ccctatgaat cgctgggaaa acattcagct cacccacgaa
     3421 aaccgacttg cgccgcgtgc gtactttttt tcatatgatt ctgttgcgca agcgcgtacc
     3481 tttgcccgcg aaaccagcag cctgtttctg cccttaagcg gtcagtggaa tttccacttt
     3541 tttgaccacc cgctgcaagt gccagaagcc ttcacctctg agttaatggc tgactggggg
     3601 catattaccg tccccgccat gtggcaaatg gaaggtcacg gcaaactgca atataccgac
     3661 gaaggttttc cattccccat cgatgtaccg tttgttccca gcgataaccc aaccggtgcc
     3721 tatcaacgta ttttcaccct cagcgacggc tggcagggta aacagacgct gattaaattt
     3781 gacggcgtcg aaacctattt tgaagtctac gttaacggtc agtatgtggg tttcagcaag
     3841 ggcagtcgcc tgaccgcaga gtttgacatc agcacgatgg ttaaaaccgg cgacaacctg
     3901 ttgtgtgtgc gcgtgatgca gtgggcggat tccacctacg tggaagacca ggatatgtgg
     3961 tggtcggcgg ggatctttcg cgatgtttat ctgatcggaa aacaactaac acatattaac
     4021 gatttcaccg ttcgtaccga ctttgacgaa gcctattgcg atgccacgct ttcctgcgaa
     4081 gtggtgctgg aaaatctcgc cgcctcccct gtcgtaacga cgctggaata taccctgttt
     4141 gatggggaac gcctggtgca cagcagcgcc attgatcatt tggcaattga aaaactgacc
     4201 agcgccagct ttgcttttac tgtcgaacag ccgcagcaat ggtcagcgga atccccttat
     4261 ctttaccatc tggtcatgac gctgaaagac gccgacggca acgttctgga agtggtacca
     4321 caacgcgttg gcttccgtga tatcaaagtg cgcgacggtc tgttctggat caataaccgt
     4381 tatgtgatgc tgcacggcgt caaccgtcac gacaacgatc atcgcaaagg ccgcgccgtt
     4441 ggaatggatc gcgtcgagaa agatctccag ttgatgaagc agcacaacat caactccgtg
     4501 cgtaccgctc actacccgaa cgatccgcgt ttttacgaac tgtgtgatat ctacggcctg
     4561 tttgtgatgg cggaaaccga cgtcgaatcg cacggctttg ctaatgtcgg cgatatcagc
     4621 cgtattaccg acgatccgca gtgggaaaaa gtctacgtcg agcgcattgt tcgccatatt
     4681 cacgcgcaga aaaaccatcc gtcgatcatc atctggtcgc tgggcaatga atccggctat
     4741 ggctgtaaca tccgcgcgat gtaccacgcg gcgaaggcgc tggatgacac gcgactggtg
     4801 cattacgaag aagatcgcga tgctgaagtg gtcgatatta tttccaccat gtacacccgc
     4861 gtgccgctga tgaatgagtt tggtgaatac ccgcatccga agccgcgcat catctgtgaa
     4921 tatgctcatg cgatggggaa cggaccaggt gggctgacgg agtaccagaa cgtcttctat
     4981 aagcacgatt gcattcaggg acattatgtc tgggaatggt gcgaccacgg gatccaggcg
     5041 caggatgaca acggcaatgt ctggtataaa ttcggcggcg actacggcga ctatcccaac
     5101 aactataact tctgtcttga tggtttgatc tattccgatc agacgccggg accgggcctg
     5161 aaagagtaca aacaggttat tgcgccggta aaaatccacg cgctggatct gactcgcggc
     5221 gaactgaaag tcgaaaataa actgtggttt accacgcttg atgactacac cctgcacgca
     5281 gaggtgcgcg ccgaaggtga aacgctcgcg acgcagcaga ttaaactgtg cgacgttgca
     5341 ccgaacagcg aagccccctt gcagatcacg ctgccgcaac tggacgctcg tgaagcgttt
     5401 ctcaacatta cggtgaccaa agattcccgc acccgctaca gcgaagccgg gcattctatc
     5461 gccacttatc agttcccgct gaaggaaaat accgcgcagc ctgtaccttt cgcaccgaat
     5521 aatgcgcgtc cgctgacgct ggaagacgat cgtttgagct gcaccgttcg cggctacaac
     5581 ttcgcgatca ccttctcaaa aatgagtggc aaaccgacat cctggcaggt gaatggcgag
     5641 tcgctgctga cccgcgagcc aaagatcaac ttcttcaagc caatgatcga caaccacaag
     5701 caggagtacg aagggctgtg gcagccgaat catttgcaga tcatgcagga acatctgcgc
     5761 gactttgccg tagaacagag cgatggtgaa gtgttgatca tcagccgcac ggttattgcc
     5821 ccaccggtgt ttgacttcgg gatgcgctgc acctacatct ggcgcatcgc tgccgatggc
     5881 caggttaacg tggcgctttc cggcgagcgt tacggcgact atccgcacat cattccgtgc
     5941 atcggtttca ccatggggat taacggcgaa tacgaccagg tggcgtatta cggtcgtgga
     6001 ccgggcgaaa actacgccga cagccagcag gctaacatca tcgatatctg gcgcagcacc
     6061 gtcgatgcca tgttcgagaa ctatcccttc ccacagaaca acggcaaccg tcagcatgtc
     6121 cgctggacgg cactgactaa ccgccacggc aatggtctgc tggttattcc gcagcgccca
     6181 attaacttca gtgcctggca ctacacccag gaaaacatcc acgctgccca gcactgtaac
     6241 gagttgcagc gcagtgatga catcaccctg aatctcgacc accagctgct tggcctcggc
     6301 tccaactcct ggggcagcga ggtgctggac tcctggcgcg tctggttccg tgacttcagc
     6361 tacggcttta cgttgctgcc gatttctggc ggagaagcta ccgcgcaaag cctggcgtcg
     6421 tatgagttcg gcgcagggtt cttttccacg aatttgcaca gcgagaataa gcaatgagga
     6481 tcatcgataa cttagaacag ttccgccaga tttacgcctc tggcaagaag tggcaacgct
     6541 gcgttgaagc gattgaaaat atcgacaaca ttcagcctgg cgtcgcccac tccatcggtg
     6601 attcattgac ctaccgtgtg gaaaatgact ccgcgaccga tgcgttattt accgggcatc
     6661 gacgctattt tgaagtgcat tactacctgc aagggcagca aaaaattgaa tatgcgccga
     6721 aagagacatt acaggtagtg gaatattatc gtgatgaaac tgaccgtgaa tatttaaaag
     6781 gctgcggaga aaccgttgag gtccacgaag ggcaagtcgt tatttgcgat atccatgaag
     6841 cgtatcggtt tatctgcaat aacgcggtca aaaaagtggt tcttaaagtc accatcgaag
     6901 atggttattt ccataacaaa taacaactac ggcggcaaaa ggagtttgcc gccaccgcta
     6961 ccctacacat tttcggagat gtgttatgtc tgataccaaa cgtaatacaa tcggcaaatt
     7021 cggcttgctc tcgctgactt ttgccgccgt ttacagcttt aacaacgtta tcaataataa
     7081 tattgagctt ggactggcct cggcaccgat gtttttcctc gcgacgattt tttattttat
     7141 tcccttctgt ctgatcatcg cagaatttgt ttcgttaaat aaaaactcag aagctggtgt
     7201 ctacgcgtgg gtaaaaagtt cgctgggcgg gcgttgggca tttattactg cctataccta
     7261 ctggttcgta aacctgttct ttttcacctc gctgttaccg cgcgttattg cttatgcttc
     7321 gtatgccttc ctcggctacg aatatattat gacaccggtt gccaccacca ttatcagtat
     7381 ggtgctgttc gccttctcca cctgggtttc taccaacggg gcgaaaatgt tggggccaat
     7441 tacctccgtc acttcaacgc tgatgctgct gttaacgctc tcctacattt tactggcagg
     7501 cacggcgctg gttggcggcg tacagcctgc cgatccgatc actgttgacg cgatgatccc
     7561 gaacttcaac tgggcgttcc tcggcgttac cacctggatc tttatggccg caggtggcgc
     7621 ggagtccgtc gcggtgtacg ttaacgacgt caaaggcggt tcgaaatcgt tcgttaaagt
     7681 gatcatcctc gccgggattt ttatcggtgt gctgtattcc gtctcctcgg tactgattaa
     7741 cgtcttcgtc agcagcaaag agttgaaatt tactggcgga tcggtgcagg tattccacgg
     7801 catggcggcg tattttggtc taccggaagc gttgatgaat cgctttgtcg gtctggtgtc
     7861 ctttaccgcg atgttcggtt ccctgctgat gtggaccgca acgccggtga aaattttctt
     7921 ctccgaaatc ccggaaggca tctttggtaa gaaaaccgtc gaactgaacg aaaacggcgt
     7981 tccggcgcgc gcagcgtgga tccagttcct gatcgtcatc ccgctgatga ttatcccgat
     8041 gctcggttcc aataccgtgc aggatctgat gaatactatt attaatatga ctgccgcagc
     8101 gtccatgctt ccgccgttat tcatcatgct ggcttacctg aatttacgcg ccaaattaga
     8161 tcacctgcca cgtgatttcc gtatgggttc ccgccgcacc ggtattatcg ttgtttcaat
     8221 gctgattgcg atatttgccg tagggtttgt cgcttcgaca ttcccgactg gcgcgaatat
     8281 tctgaccatc attttttata acgtcggcgg tattgttatc ttcctcggct ttgcgtggtg
     8341 gaaatacagt aaatatataa agggattaac ggctgaagag cgccatattg aagcgacgcc
     8401 agccagcaat gttgattaag cagactaata aaaaatgagc aggcattttc cctcttcctg
     8461 gatggggaga gggaaaatag tttctgcctt tatatcttta taattacaac gataaaaggc
     8521 tgtacttttt ctttagctca tggattaaca caatgaaatt aatcactgca ccatgcagag
     8581 cattacttgc tctgccgttt tgctacgcgt tttctgcggt cgctgaagaa gctcgtccgg
     8641 cagaacatga cgacacaaaa acacccgcca ttacctcgac atcttctcct tcatttcgtt
     8701 tttacggcga attaggggtt ggtggatata tggatttaga gggtgagaat aaacataaat
     8761 acagcgacgg cacctatatt gaaggtggac tggagatgaa gtacggctcc tggttcggcc
     8821 tgatttacgg cgaaggctgg accgtacagg ccgaccacga cggtaatgcc tgggtgcctg
     8881 accatagctg gggtggtttc gaaggtggga ttaaccgttt ctatggcggt tatcgaacca
     8941 atgatggcac cgaaatcatg ctcagtctgc gtcaggattc ctcgctggat gacctgcaat
     9001 ggtggggcga cttcaccccc gatctgggct acgtcattcc caatacccgc gacattatga
     9061 ccgcgctgca ggtgcagaac ttaagcggca actttcgtta tagcgtcacc gccacgcctg
     9121 ccggacatca tgacgaaagc aaagcctggc tacattttgg caagtacgat cgctacgacg
     9181 acaaatacac ctatccggca atgatgaacg gttacatcca gtatgacctt gccgaaggca
     9241 tcacctggat gaacggtctg gaaatcaccg acggcacagg gcaactctat ctgacgggac
     9301 tgcttacccc taactttgcc gctcgcgcct ggcaccatac cggacgcgcc gacgggctgg
     9361 acgtaccggg aagtgaaagc gggataatgg cgagcgccat gtatgaagcg ttaaaaggcg
     9421 tttatctctc caccgcttac acctacgcca aacatcgcca tgaccacgct gacgatgaaa
     9481 ccacctcttt catgcagttt ggtatctggt acgaatacgg cggcggacgt ttcgcaacgg
     9541 cttttgatag ccgcttctac atgaaaaatg cctctcacga tcccagcgac caactcttcc
     9601 tgatgcaata tttctactgg taataaggac tgtaatttca tgaaaatcaa aacaatttta
     9661 acgccagtaa cctgcgctct gctgataagt ttttccgccc atgccgctaa cgccgacaat
     9721 tataaaaacg cgattaaccg tactggcgcg ccgcagtaca tgaaggatta cgattacgac
     9781 gatcaccagc gttttaatcc gtttttcgat ctcggagcct ggcatggtca tctgttgcca
     9841 gacggcccga acaccatggg cggctttccg ggcgttgcgc tgctgacgga agagtacatc
     9901 aactttatgg ccagcaattt cgaccgcctg accgtctggc aggacagtaa gaaagtcgac
     9961 ttcacgctgg aggcatacag tattcccggc gcgctggtgc aaaaactgac agcaaaagat
    10021 atgcaggttg aaatgactct gcgcttcgcc acgccgcgca cgtcactact ggaaactaaa
    10081 atcatcagca ataaaccgct ggatctggtg tgggacggcg agctgctgga aaaactggaa
    10141 gcgaaagaag ggaaaccgct ttccgataaa accattgctg gcgagtaccc tgactatcag
    10201 cgcaaaatca gcgccacccg tgatggcctg aaagtcacct ttggcaaagt gcgcgccacc
    10261 tgggatctgc tgacctccgg tgaatcagaa tatcaggtgc ataaatccct gccagtgcag
    10321 actgaaatca acggtaatcg ctttaccagt aaggcgcata tcaacggttc gacgacgctc
    10381 tacaccacct attcccatct gctgaccgct caggaagtta gcaaagagca aatgcagatc
    10441 cgcgatattc tggcgcgtcc ggcgttttat ctcaccgcct cgcagcaacg ctgggaagag
    10501 tatctgaaga aagggttaac caatccggat gcgacgccgg aacagacgcg cgtcgcggtg
    10561 aaagcgatcg aaacgctcaa cggtaactgg cgctcaccgg gcggtgcggt gaaatataac
    10621 accgtcacgc cgtcggtgac cgggcgctgg ttctccggca atcagacctg gccgtgggat
    10681 acctggaagc aggcgtttgc gatggcgcat ttcaatccgg acatcgccaa agagaatatc
    10741 cgcgcggtct tctcctggca gatccagccc ggcgatagcg tgcgtccgca ggatgtgggc
    10801 tttgtccccg acctgatagc gtggaacctt agccccgagc gtggcggcga tggcggcaac
    10861 tggaacgaac gtaataccaa acccagcctt gccgcctggt cggtgatgga agtgtacaac
    10921 gtcacccagg ataaagcctg gttggcagag atgtacccga agctggtggc ctatcacgac
    10981 tggtggttac gtaaccgcga tcacaacggc aacggcgtgc cggaatatgg cgcgacccgc
    11041 gacaaagccc acaacaccga gagcggcgag atgctgttta cggtgaaaaa aggcgacaaa
    11101 gaagagacgc agtctgggct gaacaactac gcccgtgtgg tggagaaagg ccagtacgac
    11161 agtctggaaa ttccggcaca ggttgctgcg tcgtgggaat caggtcgtga tgacgccgcc
    11221 gtctttgggt ttatcgacaa agaacagctg gataaatatg tcgctaatgg cggcaaacgt
    11281 agcgactgga cggtgaaatt cgccgaaaac cgcagtcagg acggaacgtt gctggggtac
    11341 tcgctattgc aggagtcggt ggatcaggcc agctatatgt acagcgataa ccattatctg
    11401 gcggagatgg caaccatact cggtaagccg gaagaggcca aacgctatcg ccagttggca
    11461 cagcagctcg cggactacat caacacctgt atgttcgacc cgactaccca gttctactat
    11521 gacgtgcgca ttgaagataa accgctgccg aacggctgcg cgggcaaacc gattgtcgag
    11581 cgcggtaaag ggccagaagg ctggtcaccg ctgtttaacg gtgcagcaac gcaggccaac
    11641 gccgacgcgg tggtgaaggt gatgctcgat cctaaagagt tcaacacctt tgttccgctg
    11701 ggaacggcgg cgttaaccaa tccggccttt ggcgctgata tctactggcg cgggcgcgta
    11761 tgggtggatc agttctggtt tggtctgaaa ggaatggagc gttacggtta tcgcgatgat
    11821 gccctgaagc tggcggatac gttcttccgg cacgccaaag ggttaaccgc cgatggacca
    11881 attcaggaaa attacaaccc gctgacaggc gcacagcaag gcgcacccaa tttctcctgg
    11941 agcgccgcgc atttgtatat gttgtataac gattttttcc gtaagcagta atttgcaaca
    12001 gctggcggat gcggcgtgaa cgccttatcc gccctggccc gcggcatcgg gggtatacgc
    12061 actcttttct tttaaactgc ggtaaaaacc ggaatgcaac tgaccgaaat taataaaata
    12121 atactgattt gccgtctgac gtttaatggt ccgacggtta tgcgccctgt tcactgactg
    12181 gtgctggagc tgttgagttg aacaaacggg tcagccgctc tccggggggc cagtgagcaa
    12241 acacggcact atctgccccc ctgagagaaa ggaattcatt acatggtaac accatgccac
    12301 atcaaagcca gtgcaggtat gacattgccc ctggccctgg agaaccagga gagctgcagg
    12361 acacgtctgg aagagatgat aaatttaatc aacaggaata cagactgcag cacgggtgtt
    12421 cctgaccagg ttaacgcact ggtggctacc atggcggacg ctctgcagcc gtacacagtg
    12481 atgacgaacg aactgccgga gtatccgaac atgccggaac gtgtcctgta cattcctgag
    12541 ccgttcagtt tctgtttttc cggccacacc atcaccttca cgaacacgaa aagggattac
    12601 aacggggaca atctcgtgaa cattgcggtc agcaaggacg gggggaatgc ccgtggcgat
    12661 gctgactgtg ccctgtttca cgcctttgtc acaaaaatga tgtcacgcag tcgccagcca
    12721 ggaacatatg atttctttcc ctcactcaag gaagaaaaac aaggggaagg aatgcatttc
    12781 atggtaacac cacgcaactc cagcatcagc gcgggcgcga taatgtctcg gcccctgctg
    12841 cagacccagc acggctgtga aattctgaat aagataataa atttaatcaa cgcggatacg
    12901 gacgaggtta atgaactggt agttgccatg acgaacgcgc tgtttctgta tcagacggtg
    12961 gtgaggactg agctgcaacg cactgagcca ttcactgagc cgttcaggtt cagtttcttt
    13021 ttttccagcc acaccatcac ctttacgaag gcggagagta atagcaaatt agtgcagatt
    13081 gaggtcagta ggggcggggt gacggtccgt gaatgtgttg gcagtgcccg gtttcgcacc
    13141 attgccgcgg caatggtgtc atgccgtatc agtgaggccg ctgcaacttc cctggccctg
    13201 cagcagaccc tgcacagtcg ggtaattctg aagctgatga taaaattaat caacgcggat
    13261 acggaccgca gcatagctgt tcctgaccgg gttaacgaac tggcagttgc catgacgaac
    13321 gcactatatc ggtaccagac ggtgaggggt gataagcaga taatccctga atcgttcagt
    13381 ttcgggtttt ccggccatac catcaccgtc acgccgggaa ggtatagtcg ctctgtgaac
    13441 cttaaggtct gtgggggcgg ggtgccagct tgtgcaacag ctgaccgtac tctgtttcgc
    13501 accattgcca cagcactggt atcaagcaac cagcaaggaa cacttgattt cagatcctcc
    13561 ctccccttcc tggtggaaag aaac
//
LOCUS       DACBVI010000120        13309 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.120, whole genome shotgun sequence.
ACCESSION   DACBVI010000120 DACBVI010000000
VERSION     DACBVI010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.120"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            5..829
                     /locus_tag="IU325_17005"
     CDS             5..829
                     /locus_tag="IU325_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAP5810699.1"
                     /translation="MPLSNTLQSQIITDNHFLHHPKVDSELTRKYERARLDTENIYLL
                     PLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKITS
                     ALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYAGT
                     TIGYISTLPVSQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGNDP
                     IIGIEAYPGTAEIHAKMGYKVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK"
     gene            complement(970..1743)
                     /gene="uxuR"
                     /locus_tag="IU325_17010"
     CDS             complement(970..1743)
                     /gene="uxuR"
                     /locus_tag="IU325_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HAP5810700.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSSDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDV"
     gene            complement(1958..3418)
                     /locus_tag="IU325_17015"
     CDS             complement(1958..3418)
                     /locus_tag="IU325_17015"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HAP5810701.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3499..4683)
                     /gene="uxuA"
                     /locus_tag="IU325_17020"
     CDS             complement(3499..4683)
                     /gene="uxuA"
                     /locus_tag="IU325_17020"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP5810702.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5023..6366
                     /gene="gntP"
                     /locus_tag="IU325_17025"
     CDS             5023..6366
                     /gene="gntP"
                     /locus_tag="IU325_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HAP5810703.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6539..7441)
                     /gene="fimH"
                     /locus_tag="IU325_17030"
     CDS             complement(6539..7441)
                     /gene="fimH"
                     /locus_tag="IU325_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HAP5810704.1"
                     /translation="MKRVINLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVVIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIAVTFVYQ"
     gene            complement(7461..7964)
                     /gene="fimG"
                     /locus_tag="IU325_17035"
     CDS             complement(7461..7964)
                     /gene="fimG"
                     /locus_tag="IU325_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HAP5810705.1"
                     /translation="MKWCKRGYVLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNSGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7977..8507)
                     /gene="fimF"
                     /locus_tag="IU325_17040"
     CDS             complement(7977..8507)
                     /gene="fimF"
                     /locus_tag="IU325_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HAP5810706.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPINAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8517..11152)
                     /gene="fimD"
                     /locus_tag="IU325_17045"
                     /pseudo
     CDS             complement(8517..11152)
                     /gene="fimD"
                     /locus_tag="IU325_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein FimD"
     gene            complement(11219..11944)
                     /gene="fimC"
                     /locus_tag="IU325_17050"
     CDS             complement(11219..11944)
                     /gene="fimC"
                     /locus_tag="IU325_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HAP5810707.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11981..12520)
                     /locus_tag="IU325_17055"
     CDS             complement(11981..12520)
                     /locus_tag="IU325_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP5810708.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12585..13139)
                     /locus_tag="IU325_17060"
     CDS             complement(12585..13139)
                     /locus_tag="IU325_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP5810709.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAETTPTTVNGGTVHFKGEVVNA
                     ACAVDAGSVDQTVQLGQVRTASLKQTGATSSAVGFNIQLNDCDTSVATKAAVAFLGTA
                     IDSAHPKVLALQSSAAGSATNVGVQILDRTGNELTLDGATFSAQTTLNNGTNTIPFQA
                     RYYAIGEATPGAANADATFKVQYQ"
BASE COUNT         3225 a         3472 c         3207 g         3405 t
ORIGIN      
        1 gtctatgcca ttaagcaata cccttcagag ccaaattatt acagataatc acttccttca
       61 tcatccaaaa gtcgacagtg agttaacacg taaatatgaa cgtgcacgcc tggatacgga
      121 aaatatctat cttctgcctc ttgctcgtgg caataaccat aattacgatg gaaaaagcgt
      181 tgtcgaaatt agaaaacttg atattagtaa agagtcctgg ccatttaatt atgtgacaga
      241 agcatgcaga gaatccgatg gtattacaac aacaggacgt atgctctatc gaaacttgaa
      301 aatcacctca gctttagacg aaatatatgg tggcatatgt aaaaaggcgc atgctgctac
      361 agaattagca gaaggtttac ggctaaattt atttatgaag agccctttcg atcctgttga
      421 ggactataca gttcatgaaa taacattagg ccctggttgc aatgttccag gttacgccgg
      481 aaccacgata ggatatattt caacccttcc agtttctcag gcaaaaagat ggaccaatga
      541 gcagccaaga attgatatct atattgatca gataataact gtctcagggg tagcaaattc
      601 ttccggattc gcccttgccg cattacttaa tgctaatatt gagatgggaa atgacccaat
      661 cattggtata gaagcctatc ctggtaccgc ggagatccat gcaaaaatgg gatataaagt
      721 tatccccggt gatgaagatg cgccactcaa acgcatgact ctgcaaccgt catctcttcc
      781 agaactgttt gaattaaaaa atggtgaatg gaattatatt ggtaaatagc cactgagaag
      841 aatgagtact aatattcagg ttatacagat atttaaacaa taccctgcaa tttacaaagc
      901 aatactcatt gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat
      961 tataataagt cagacgtcga ctttatccag cggccatgaa tcaaacagat agccgtcaaa
     1021 ataaatatcg tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg
     1081 ccacattgcc agcttcgccg ctcgggcatc ttttttgatt aacgcggcga ggatctgttt
     1141 gtgatcgccc aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat
     1201 ccacattgga ttgttttccc gccactgcca ggactgacgg aataactcca ccagcatgct
     1261 gttatgcgtt gcttcggcaa tggcgagatg gaactgcata tcaccgcttt cgctgctacc
     1321 cggcgcgctg gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat
     1381 atcttcgcgg gtagcctgca aagcggcaaa ttccgcgatg ttactttcca gtaactgacg
     1441 cgcctgtaac agctcaaaag gacccgcatc attacagacg ttggcatccg aactgtctgt
     1501 gttgtggctg cctgagctat caagaacata gataccggca ccccggcgca cttccaccag
     1561 ccctttgatc tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat
     1621 ttctgcaatt tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat
     1681 cagatcgcgg atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tggcagattt
     1741 catggtgttg tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc
     1801 cttatccggc ctacaacggg caattttacg gcccctgtta atctcaaact ataccattag
     1861 cactggaagt tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac caaatatcag
     1921 tttgattgac cagtaagtct gtagggtaat cagttagtta cagcgcagcc acacactcgc
     1981 gcgcaccgcg ttcgcacagc tgctgatatg ccgccgtcac cgcgccaaca aagtcggcat
     2041 tctgcggcag atcatcggca aaaataccgc tcaggcctag cagcgctttc acgcggtctg
     2101 cgccctgata ctgcgcgtta atcttctgga actcggccag catcgggtcg accacgtcaa
     2161 tggcattacc ctgctcatcc acgccctggg tgtaacgcat ccagccagcc acgcccagcg
     2221 ccaggtgacg ccagctgccg ccgttttgca ggtgcagacg caccgggtcc agcagacgct
     2281 gcggtaactt ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt
     2341 tgctgaaacg ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct tccggcatcg
     2401 acagcgttgg cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga taatccgggt
     2461 tagtcatggt gtcggcaata gtttcatagc cgccgaggta accgaggtac gccaggaaag
     2521 agtggctacc gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga
     2581 actgtgcacc cactttatcc cagtccgggc gaccattaac gaaattatct tcaatcaccc
     2641 actgacggaa cggttcgcag gcaatggcgc acgggtcgta aacgcccagc tggtcagcaa
     2701 tttcctgtaa ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga
     2761 aggtgacgtt ctcttcaatc catgccgcta gctgcgggtc acgcgcctga gccagcccca
     2821 gtaccgcgac cttcgccaca tgaccgtttt cacgcacgtt atcgcaggac atcaccgtaa
     2881 atgctttcag ccctttttca cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg
     2941 acttcggcgc agtcgggttt tccagatcgt gcttgatcag cgggttattg agatccagtt
     3001 gaccgcttgc cgcatcagcg cagtagcctt tttccgtgac cgtcagagag acaatcgccg
     3061 tttgcggacg cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc gggtgcagcg
     3121 cttctttcat cgaaccgata attttcagct cggtgctttc cgcgcctttt tccgccacgg
     3181 tgtacagcag ttgctgtttt ttcaggtttt cgatcagcac gcggtcgttg cctggcatca
     3241 ggttaacttc gcagataccc cagtcgctgt cggtgctttc cagcagatga tgggtgtaca
     3301 gcgcctggtg cgcacggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac
     3361 gagaatgatc ccatgacggg cgggcaaccg gcagattgct gtcaacaata gtagtcatcg
     3421 taaactccag aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg
     3481 cagccatgcc ggtggatatt aacggctaaa gaaagcgcgc tggatcgcca gttcgacacc
     3541 gcgaacttcg gccaggcctt tcagacgacc aattgcggag taacctgggt tggttttctt
     3601 cttcaggtcg tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt
     3661 gccttccgct ttacgacggt gttcttcttc aacaatcgct ttcaccactt cgtacatatc
     3721 aacgtcaccg ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt
     3781 ggagcgcaga tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc
     3841 gttgtcagca cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctatt
     3901 tacggtatca accatccact gcatatcttc aatggtagaa acaatgcgcg gcaggccgag
     3961 gattgggcgc ggcggatcgt ccgggtgaac agccatacgc acgccaactt cttcggcaac
     4021 cggaataatc gccttcagga agacggcaaa gttttcgcgc agtttggctt tgtcgatatc
     4081 tttgtacagc tccaggtgct tacggaactg gtcgagggtg tacccttctt ccgcgcccgg
     4141 cagaccggca atgatgttac gggtcagacg cgctttgtct tcgtcgctca tagtggcgaa
     4201 gcgttcagct gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcac ctgggcgttt
     4261 caggatatgc atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc
     4321 gtctggcagc acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta
     4381 gcacacggtg cgaataccgc actgcgccag gttgcgcaga gtctgctgat agttagcgat
     4441 ccactgctca tagttgccag tgtgggtttt gatatcttcg tgaattggca cgctttctac
     4501 gacagaccac accaggcctg catcttcaac gatagccttg cgtttgagga tctcttctac
     4561 ggaccatact tcaccgttcg ggatatggtg caaagcggta accacgccag tcgcgcccgc
     4621 ctgacggaca tcagctaaag aaaccggatc gtttgggccg taccagcgcc aggtctgttc
     4681 catgattttt cctctttcac atagtgggtt ccacagcaaa gagacgcgga accctggagt
     4741 tgtttaggta tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gatgagatgg
     4801 cgtataagtt ctaccttttg gtaagacatg tcaacccgaa ttctgaaatt ggttaaccac
     4861 atcacaagaa tttcactctc ctggttgttg ctgcaaattg cagcaaaagg taatcgatgg
     4921 ttattgatca gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt
     4981 cgcccaaaat aacaacggtt caaccaggta aggtaaaacg ttatgcatgt gcttaacatt
     5041 ctctgggtgg tattcggcat tggtctgatg ctggtactga atttgaagtt caaaatcaat
     5101 tcaatggtgg ccttgttggt ggcggcgctg tccgtcggga tgctggcggg catggatttg
     5161 atgtcgctgc tgcacaccat gaaagcgggc ttcggcaaca cgctggggga actggctatc
     5221 atcgtggtgt tcggtgcggt catcggtaaa ttgatggtcg actccggcgc ggctcaccag
     5281 atagcgcata cgctgctggc acgtctcggt ctgcgttatg tacagctgtc ggtgattatc
     5341 atcggcctga ttttcggtct ggcgatgttc tatgaagtgg cctttatcat gttagcaccg
     5401 ctggttattg ttattgccgc cgaagctaaa attccgttcc tgaaactggc gatcccggca
     5461 gtagcagctg ccactaccgc acattcactg ttcccaccgc agccgggtcc ggtggcgctg
     5521 gtgaatgctt atggcgcgga tatggggatg gtttatatct atggcgtact ggtgacgatc
     5581 ccaagtgtca tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct tgagcgccca
     5641 acgccatcat tcctgaaagc agatcaaccg gtagatatga ataatctgcc ctctttcggc
     5701 gtttcgattc tggtgccgtt gatcccggcg atcattatga tctctaccac catcgccaat
     5761 atctggctgg taaaagatac ccctgcgtgg gaagtggtta actttatcgg ttcctcgccg
     5821 attgcaatgt ttattgcgat ggtggttgca ttcgtactct ttggcaccgc gcgcgggcac
     5881 gatatgcagt gggtaatgaa cgcttttgaa agcgcggtga aaagcattgc aatggtgatt
     5941 ctgatcatcg gtgcaggtgg cgtgctgaag cagaccatca tcgacaccgg catcggcgac
     6001 accatcggca tgctgatgtc ccacggcaat atctcgccct acatcatggc atggctgatc
     6061 accgtactga ttcgtctggc gacgggtcag ggtgtggttt cagcgatgac cgccgccggg
     6121 attatcagtg cagcaatcct tgatccagca actggtcagc tggttggcgt gaatccggcg
     6181 ctgctggtac tggcgacggc tgcgggttcc aacaccctca cccacattaa cgatgcctca
     6241 ttctggttgt tcaaaggtta ctttgacctg tcagtaaaag acacgttgaa aacctggggt
     6301 ctgctggagc tggtcaactc cgtggttggg ctgattattg tgctgattat tagcatggta
     6361 gcgtaaaaga gcacctgagc ctgccattgg caggctctgt tcggattgtc ggtaaagtgc
     6421 gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa gggctaacgt
     6481 gcaggtttta gcttcaggta atattgcgta cctgcattag caatgccctg tgatttcttt
     6541 attgataaac aaaagtcacg gcaataatcg attgcacatt ccctgcagtc acctgccctc
     6601 cggtacgtgc gtaatttgcc gttaatccca gacttaccgc cgaagtccct actgctccta
     6661 acgataccgt gttattcgct ggaataatcg taccgttgcg cgtcaactgt acgccgacgc
     6721 cctgtgcagg tgaaaacgac gcggtattgg tgaaaatcga gttgcccgca tctgcggttg
     6781 tgccggagag gtaatacccc aggttttggc ttttcgcaca ataaacggta agaggaatcg
     6841 gcactgaacc agggtagtcc ggcagagtaa cggtgacatc acgagcagaa acatcgcagc
     6901 cgccagtggg caccaccaca tcattattgg cgtaaatatt ccacacaaac tggaaatcat
     6961 cgctgttata gttgttggtc tgtcgcaaaa taagcacggc aattaatgag ccagctttaa
     7021 tcaccacccc gcccgcactg ctcacaggcg tcaaataaag cgccaccggc cacggcttat
     7081 ccgttctcga attataaaca acccgcggcg tttcactggt ggtcggaaat ggatagctac
     7141 tgccactata ttttacggtc ccggaaaaat tagataacac gccgccataa gccgagcctc
     7201 gttgcagtgt gacataatct gtaatggttt ccgggtaatc gttatggcaa aagatttgcg
     7261 tcgaaagatc cacgaccagg ttttgcccca cattcacggc gggcgcaagg tttacataaa
     7321 cattggcgct gccaccgcca atagggatag cggtgccatt ggcggtttta caggcgaatg
     7381 accaggcatt taccgaccag cccatcagca gtacagcaaa caggttaata actcgtttca
     7441 ttacaatcat ctcttcggct tcagctgtag gtataggtga tgctaatcac tgcctgaatg
     7501 gttccctgag tggctccgcc atttaccgtc aatgctctga cctgtaacgg gaagtgcgct
     7561 gattgtgagg aatcatccac ctgaactgtt ttggttgcgc cactattcaa tgtgttgcca
     7621 ctgtcatcct gtagctctaa ctggatgttt tgcgcggtcc cctggttttt ataatatccg
     7681 gtactgtcgg ctgccccgct gaagctggca gtgaccctcg acgttcccac cggacaatta
     7741 gtcaactcaa gcgcaacatc atgccaggcc gatgccgccc cggcagacat cagactgaaa
     7801 gaataaagat cgccgagatc taccgtggca ttggtggtgg aaaccgtgca cggtttggcg
     7861 acgaccttac cgttcaccgt gatggtgaca tcggctgcct gtatcgtcgc acttgcgagc
     7921 gccaatattg ccgccaatac atacccacgt ttgcaccatt tcatgagcat ctccagttac
     7981 tgatattcaa gagtgaaggt agccgtggca ttgatatgcc ccgcagtgac aggcacctgt
     8041 gtcgccatta gccgggcgta aaaattcagc gtatttggtt tacccggcgt cagggtcgtc
     8101 cacgaaatcg cggacgatgg ggcattaatg ggtatctgat tttgttgctc attcagaagc
     8161 tgtattccca gtcccgcagc cgctgacacc gtattttcaa gtgcaagcag gttggcattg
     8221 tggctatctg caacaccggt aaacccaact tttacggcag aaacggcatt accacagggt
     8281 gacagcaaaa tacgaaatgg aacgacagga gtcgtcgcgc caatgttgtt aaattgcttc
     8341 gccgcgtttt ccatcagatc aacagtaaaa ttggttgatt cagcggccac actacagccg
     8401 ttatctctga catagccgcg gatagtaatc gtgctatccg cagccaaagc gtgacttacc
     8461 gccagccaca aaaaagcgca cagaagataa aaaggtttgt ttctcatcac gcccccttaa
     8521 cgacattcag ctgatagctg ggttaataac tgctgctgac tctctggtgg cagttgataa
     8581 ttggcgacac agtgagcatt ttcctcttct ccccatttca cctgaacttt tcccgctaaa
     8641 ggcattccgc tgaggtaaac ctgaccatta tccgcaacaa tgccgctact ctggctactc
     8701 tctgatgtca ccatcgcccc aaacggcagc ggcttattat tgtgggttag cgtcatgagc
     8761 agttttatcc caacgcgcgc tttaaactct gctcgcacga tcgccccacg ggtgggaaca
     8821 acgttagcga ccgcgttatc taaatcgacg ttatcagcca gggtattggt atccagcgct
     8881 actctgttct cccgatattc agtggcataa ggcagcacgg cataaccgcg ccagtcggta
     8941 cgcacccccg tctggttttc gacttttaca tcttttgcgc caggcgcttt aacaagcacc
     9001 accgtatcgt ttaacggctg ccccagcgtt acgccattgg catgagccag taccccaccg
     9061 ctgactccgt aatagagctg cttaatatca tcgctatggc tgtaaccgat gttggcattg
     9121 ccgtaaccac cgcgataatt cagcgtggcg tagcctgtac ttccgctatt accatcacct
     9181 cccccggcgt aaccggtttg cacgctatag ctgaggttgt tgtcttccag caacgtaccg
     9241 tatacaccga ccagattggt catccgaccg ttgagatcgt gtgacatgct gtaactggca
     9301 ctggcatgtc gccactgaga tttactgtca gaacgcagcc agtggctgaa aggaatattg
     9361 acgttaagcg ctaacatctg atcccgtcct ttttgccagg cgtttttcgt caggctatag
     9421 ctgagcgtcc agttgatatc ttcgaatgca gtatttaaac cagcctggaa ttgctcatcg
     9481 acattactcg ttccccaata agtttgatgg ctaccactca aatacagtgt tgatgtgcgc
     9541 ccgagttgct gagtaacggt gagttgtaat ttcccgcgtt tgttataagc gagattgtaa
     9601 tagtcggtga atttcggctt aacctgaata acaccatcct gtgtttcgat gttgtagcca
     9661 ttcattcgac tgtatgttgt atcagcgaat taaaatatcc gctggtcgaa taacggtaac
     9721 ccactaactg aatattcgtg cctgattcat tgagcgattt gttatagaga aaacgcaccg
     9781 attgtccgtc atgctgactg tcatcgggaa gcgtggaatt agcctgcgtc atatccacag
     9841 acagagcgcc cagtgccccc atgtttttcc cgataccgaa attaaacgca cgataacgat
     9901 ccgccagttg cgttccacca tatattgtcc agccagccgg aaggccgtgg agtaatgtac
     9961 tctggaaaaa gcggggtttt tcctgctgcg catttccact acggtattct cctgccgtaa
    10021 tggaataacg agtatgccct tcacgttgca aaagcgggac tgacgaatag ggtacggtaa
    10081 agatctgcgt gctgccgtca gcctctttaa tcgttacctg caagtcacca ctattacctg
    10141 cggcatagat atcgttgatg gtaaaaggcc ccggcggcac cgtactatta taaatgtcat
    10201 acccattttg tttaatagtg acctgtgcag taccacgagc aataccgtgg atcaccgggg
    10261 caaatcctct ttggctatcg ggtaacatat tgtcatctga ggccaattgt gcgccgcgaa
    10321 agttaatacc atcgaaaata tcgccctggg tataaccatc acccagcgtc agccgggaac
    10381 gtaacggaat tatgtctcgc tcaagccagg tattgatatg ctgccattta tttttgctac
    10441 ctgatgaact gtcgctactg ttataactcc aggtggtatt gtcgcgtaaa caccacgcac
    10501 caatatttaa cccactctgt aggtttaaat atgcataatg gctgttaccc ccaatccgat
    10561 tctgtacact atttccgctg aaattataat tgagcaatcc ggcattaata ccaggatccc
    10621 ataactcagg aggaatataa ccacgcgcgc gattactcat aaatgcctga gggatcgtca
    10681 ggttgagtcg ctgctgacca acatccagat gcgcagtagc gtcatggatc attgaggtta
    10741 atggcacgca ggcatcatcc gccagcagat tcataccgga gacagaagcc gtattcagcc
    10801 ccatacttgc aagttgcgcg cgtgtcaggc agggaacaat cccttgttca ctgtcgcccg
    10861 tattaaatgt gacatcacgc gttgccatat aaccattatt caaatagata tcgacacgat
    10921 acgtccctgg cggtaattct tgcccatttt caaaacgcga taaatcggcc acagcctggg
    10981 gctcatccgc taaaaagcgc ggattaaaat agagttcggc agatgacaaa ggtgcctgtg
    11041 cggcaaaagc acaggcgaca aagagccgga caaaaaaacc agccaaacga tgcttacgaa
    11101 tatgcaagca ttgtgtgttt cgctggtaaa gtcttaaatt cagatatgac attattcctc
    11161 cggtttaaaa tcacccggca gtcaattctt tttattcagg cgaaaaattc cccctgcgtt
    11221 attccattac gcccgtcatt ttgggggtaa gtgcgccata atcatttatt gttcggtaag
    11281 taatattgct tcctgcatca gaaggcaatt taaccgtgct ttcgcccatt ggaggcacca
    11341 atgcattttc aagaacccgg gttccggcat tcaactctgt taccgtcagg taatagggtg
    11401 tcgggttaat cagagtcaga gaattcgcgc tacgacgaaa tcttaatttt tctgcggcct
    11461 gatcgggtgg caacgctaat ttagccgggc gatagtacag tttaatacgg ctgataattg
    11521 cgagctgtag cgtattctca gtcaattttg atttatccat tgacggaatc gctttaacgt
    11581 tcatccagaa taaactttcc cgatcctgtg gcaattggtt atttgttgca tcaagaatac
    11641 gtaaggtatt ctcttttttt cccttcatcg caaacagagg tggcgtcacg ataaaacgac
    11701 catcctttac accatcggca ttttccaccc atgattgaat taaataggtg ctattttcat
    11761 cattatttgt cacggcaagt tgcacttgtt tttgccctgc cggataaatt acgcgagtcg
    11821 cacctaaggc cactcccgct tcggcgcgtc cggcaaccat cattgccatg aacatcagga
    11881 tacctgccag caagcagaat gttatttcct gcgatttcct tacattgacg tttttattac
    11941 tcactgtcct gttcctgtta tcacattatc tgctgaacaa ttactgatag gttaaagaga
    12001 accaggcctg ggcattggcg atgccgccag taacccgacg cccggtagca cgatatttgg
    12061 cgatgaaatg tagcgaagta gagcctgagt aaagccgttt ccagtttgct ggaggacgat
    12121 taatcggtac gaggtttcct tcatcatcaa acaacgctac gccaatattg gtggctatcc
    12181 ctggcccctc tcccacggaa agcacatccg gatttttacc atccgcgaca ccgtgaaacg
    12241 ccacacccac acgttcactc accaccgtgc tacattcccg taaatgaata acaaaaggca
    12301 ccggtgcgct atcttcccct accgcatgaa accggttact gctgatttgc cccatattga
    12361 ccgtcatttg tttatcaccg gcttcaatcc ggcaagtttc cgcaataatg acgccctgaa
    12421 attgcatatt tccgccgggc agcgtggtgt tccatttatt tccggccaga gcaaacattg
    12481 gcagcaacga cgctaataga aataatctct tccttttcat cgttattttt atcgcacaag
    12541 ggtgggcatc cctgcccgta atgacgtccc taaacctggg taggttattg atactgaacc
    12601 ttgaaggtcg catccgcatt agcagcaccc ggggttgcct cgccgattgc ataataacgc
    12661 gcctggaacg gaatggtgtt ggtaccgtta ttcagggttg tttgtgcact aaatgtcgca
    12721 ccgtccagcg tcagctcatt tcctgttctg tccagtatct gcacaccaac atttgttgcg
    12781 ctacccgcag ctgaactctg cagagccagt actttaggat gagcactgtc aatcgcagtc
    12841 cccaagaagg caacagcggc ttttgtggca acactggtat cgcaatcatt cagctgaatg
    12901 ttaaaaccga cagcagagct ggttgctcca gtctgcttca gactagcggt acgaacctgg
    12961 cccaactgaa cggtttgatc aacagagcct gcatcaactg cgcaagcggc gttaacaact
    13021 tcccctttaa agtgaacggt cccaccattt accgtcgtgg gtgtagtttc agccagagcc
    13081 gctgtagaac tcagggacag agccgacaga acaacaattg ccagagtttt aattttcata
    13141 ctgctttcct ttcaaaaaac tatttctaaa tcgacatggg cagtcgttcc gtacactttg
    13201 ttttgtcaac gagtttgact gccaacactg cacagtttcc cccccaaaag atgaaacatt
    13261 tggggccatt ttgactcata gaggaaagca tcgcgcacaa actttttca
//
LOCUS       DACBVI010000121        13189 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.121, whole genome shotgun sequence.
ACCESSION   DACBVI010000121 DACBVI010000000
VERSION     DACBVI010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.121"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            130..372
                     /locus_tag="IU325_17065"
     CDS             130..372
                     /locus_tag="IU325_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810710.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            486..1124
                     /locus_tag="IU325_17070"
     CDS             486..1124
                     /locus_tag="IU325_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810711.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     DVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEDVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1121..1858
                     /locus_tag="IU325_17075"
     CDS             1121..1858
                     /locus_tag="IU325_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810712.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTLGRDVASYLSGQNLLSSADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSKTPDALNADILQTLTQRIPQ"
     gene            1858..3954
                     /locus_tag="IU325_17080"
     CDS             1858..3954
                     /locus_tag="IU325_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HAP5810713.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4001..4279)
                     /locus_tag="IU325_17085"
     CDS             complement(4001..4279)
                     /locus_tag="IU325_17085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810714.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKTDLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4280..4423
                     /locus_tag="IU325_17090"
     CDS             4280..4423
                     /locus_tag="IU325_17090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810715.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFAVPAMVRFVLPDAPPGAAYP
                     AYG"
     gene            4551..4961
                     /gene="psiE"
                     /locus_tag="IU325_17095"
     CDS             4551..4961
                     /gene="psiE"
                     /locus_tag="IU325_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HAP5810716.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5055..5945)
                     /gene="malG"
                     /locus_tag="IU325_17100"
     CDS             complement(5055..5945)
                     /gene="malG"
                     /locus_tag="IU325_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HAP5810717.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(5960..7504)
                     /gene="malF"
                     /locus_tag="IU325_17105"
     CDS             complement(5960..7504)
                     /gene="malF"
                     /locus_tag="IU325_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HAP5810718.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSTLFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(7658..8848)
                     /gene="malE"
                     /locus_tag="IU325_17110"
     CDS             complement(7658..8848)
                     /gene="malE"
                     /locus_tag="IU325_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HAP5810719.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGHPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            9213..10328
                     /gene="malK"
                     /locus_tag="IU325_17115"
     CDS             9213..10328
                     /gene="malK"
                     /locus_tag="IU325_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HAP5810720.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            10400..11740
                     /gene="lamB"
                     /locus_tag="IU325_17120"
     CDS             10400..11740
                     /gene="lamB"
                     /locus_tag="IU325_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HAP5810721.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGNANTNTNFGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            11892..12812
                     /gene="malM"
                     /locus_tag="IU325_17125"
     CDS             11892..12812
                     /gene="malM"
                     /locus_tag="IU325_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HAP5810722.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
BASE COUNT         3178 a         3431 c         3499 g         3081 t
ORIGIN      
        1 attccaatta tcctaaaacc ccatcgctaa ttccccacac cgtaattcgc atgctttagt
       61 tgtgtatact cgatcccgcc cgaaatgttt ttgggtaaat ctccattcat tcaatgaagg
      121 gaaattgtta tgaaaaaagt tctgtatggc atttttgcca tatctgcgct tgcggcgact
      181 tctgcgtggg ctgcacctgt acaggtgggc gaagcggcag ggtcggcagc aacgtcggtt
      241 tcggcgggga gttcctccgc gaccagcgtc agcaccgtaa gctcggcggt gggtgtcgcg
      301 ctcgcggcaa ccggtggcgg tgatggttct aataccggga ccacaacaac cacgaccacc
      361 agtacccagt aataaagtat gtatccccaa aataattcga gtcattgcat ctgtggcttg
      421 aagtatgaag ggatttacca taaccacact ccggtgtggt tattctgccc ctctggagaa
      481 gagtcgtgaa gcgacctgca ctcattctta tctgcctgct attacaggcc tgttcagcca
      541 cgactaaaga gctgggcaat tcgctgtggg acagtctgtt cggcacgcca ggcgtacagc
      601 tgacggacga tgatattcaa aatatgccct acgccagcca gtacatgcag cttaatggcg
      661 ggccgcaatt gtttgtggtg ctggcctttg ctgaagacgg acaacaaaaa tgggtcactc
      721 aggatcaggc cactctcgtc acacaacatg gtcgtctggt gaagactttg cttggcggcg
      781 acaacctgat tgacgtgaat aaccttgccg ccgacccgct gattaaaccc gcacaaattg
      841 ttgatggtgc aacatggacg cgcacgatgg gctggaccga gtaccagcag gtacgctacg
      901 ccaccgcacg ctcagtcttc aaatgggatg gcaccgatac cgtcaaagtc ggcagcgatg
      961 aaactccggt tcgcgtgctg gacgaagacg tctccaccga ccaggcgcgc tggcataacc
     1021 gctattggat cgacagcgaa gggcaaattc gccagtcgga acagtatctc ggcgcggatt
     1081 atttcccggt gaaaaccact ctcatcaagg cggcaaaaca atgattaaac aaactattgt
     1141 cgcattgatt ttgagtgtgg gggcgtcatc ggtcttcgcg gcgggaaccg tcaaggtatt
     1201 cagcaatggc agcagtgagg ccaaaacgct gacgggggca gagcatttaa tcgatctggt
     1261 aggccaaccg cggctggcaa acagttggtg gcccggtgcg gtgattagcg aagagctggc
     1321 aacggcggca gcattgcgtc agcagcaggc gttgctgaca cggctggcag aactagccgc
     1381 agattccagc gctgacgatg ccgccgccat taacgcctta cgccagcaaa ttcaggcgtt
     1441 gaaggtgacg ggcaggcaaa aaatcaatct tgatcccgat atcgtccgcg ttgctgaacg
     1501 cggtaacccg ccgttgcagg gcaactacac gctgtgggtc ggaccaccgc cgtccacggt
     1561 cacgttattc gggcttatca gccgtcctgg caagcagcca ttcactctag gtcgcgacgt
     1621 cgcaagctat ctctccggcc agaacctgct tagcagtgcg gatcgcagct acgcgtgggt
     1681 ggtttacccg gacggacgca cgcaaaaagc gccggtggct tactggaaca agcgtcacgt
     1741 agagccgatg cccggcagca ttatttatgt tggcctcgcg gactccgtct ggagtaagac
     1801 gcctgatgcc ctgaacgccg acattcttca gactctgacg cagcggatac cccaataatg
     1861 aaaaaaagac atctgcttag cttactggcg ctgggcatta gcacagcttg ctacggcgaa
     1921 acatatcctg cgcccattgg tccgtcgcag tcggatttcg gtggcgtagg attattacaa
     1981 acgcccaccg cacgtatggc acgggaaggg gagctgagcc tgaactatcg cgataacgat
     2041 cagtaccgtt attactcagc ttcagtgcaa ctcttcccgt ggctggaaac aacgctgcgc
     2101 tacaccgacg tgcgcacccg gcagtacagc agcgtcgaag cgttctctgg cgatcaaacg
     2161 tataaagata aagccttcga tctcaaactg cgtttgtggg aagagagtta ctggctgccg
     2221 caagtggcgg ttggtgcgcg ggatatcggc ggtacggggc tgtttgatgc ggaatatctt
     2281 gttgccagca aagcctgggg gccgttcgat tttacgctcg gcctgggatg gggatatctg
     2341 ggcaccagcg gtaatgtgaa aaatccgctc tgttcagcca gtgataaata ttgctatcgc
     2401 gataacagct acaaacaggc gggatctatc gacggtagcc agatgttcca cggtcctgcc
     2461 tcactgtttg gcggcgtgga ataccagacg ccctggcaac cgctgcgcct gaaactggag
     2521 tatgaaggca ataattatca gcaggatttt gccgggaagc tggagcaaaa aagtaagttt
     2581 aacgtcgggg cgatttatcg cgttaccgat tgggccgacg ttaaccttag ctatgaacgt
     2641 ggcaacacct ttatgtttgg cgtcacgctg cgcaccaact ttaacgatct gcgcccgtcc
     2701 tacaacgata acgcccgccc gcaatatcaa ccgcagccgc aggatgccat tttgcagcat
     2761 tcggtggtgg caaatcagtt aacgctgttg aaatacaacg ccggactggc cgatccgcag
     2821 atccaggcga aaggcgatac gctgtatgtc accggcgagc aggtgaaata tcgtgattcg
     2881 cgcgaaggga tcatccgtgc taatcggatc gtgatgaacg atctgccgga tgggatcaaa
     2941 acgatccgca ttacggagaa tcgccttaac atgccgcagg tgacgacgga aaccgatgtc
     3001 gccagcctga aaaatcatct tgccggagag ccgttgggcc acgaaacgac gctggcgcaa
     3061 aaacgcgtcg agccagtggt tccgaagtcc accgagcagg gctggtatat cgacaaatca
     3121 cgctttgatt tccatatcga tccggtgctg aaccagtcgg tcggtggccc ggaaaacttt
     3181 tacatgtatc agctgggcgt gatgggaacg gcagatttgt ggctgacgga ccatctgctg
     3241 accactggaa gcctgtttgc aaatcttgcc aacaactacg acaagtttaa ctacactaat
     3301 ccaccgcagg actcgcactt accgcgcgtt cgtacccatg tgcgcgagta tgtgcagaac
     3361 gatgtctatg tgaataacct gcaagccaac tacttccagc atctgggcaa cggcttctac
     3421 ggtcaggtct acggtggtta tctcgaaacc atgtttggcg gtgcgggggc agaagtgttg
     3481 tatcgcccgc tggacagcaa ctgggcgttt ggtctggatg ccaactacgt taaacagcgc
     3541 gactggcgta gcgcaaaaga tatgatgaaa ttcaccgact acagcgtgaa aaccggacat
     3601 ctgaccgcat actggacgcc atctttcgct caggatgtgt tagtgaaagc gagcgtcggg
     3661 cagtatctgg caggggataa aggcggcacg ctggagatcg ccaaacgctt tgatagcggc
     3721 gtggtggtgg gtggctatgc cacgatcacc aatgtttcga aagaggagta cggcgaaggg
     3781 gacttcacca aaggcgtgta tgtctctgta ccgctggatc tcttctcgtc tggcccgaca
     3841 cgcagccgtg cggcaattgg ctggacgccg ctgacgcgtg acggtggtca gcaacttggg
     3901 cgtaagttcc agttgtatga catgaccagc gaccgtagcg tcaatttccg ctaagtcatg
     3961 ggaaaggtgc cagttttcgc actcactggc acctttattc ttaatggcct gtcgagccat
     4021 caggaacgca gttcaccccc ggtttgcgat ctggtgtgta aaggcgaaaa cattctgtct
     4081 tcctgtccgt cgatattggt tgcaggtctg gttgcctgag cgactgggcc atcattgggc
     4141 tggagacatt gctttgcggt aggtcggttt tatcagcagt accgagcgga ccagcataag
     4201 caggaagaac agagactgat aacatcaaag cagcaaaata aggcttcatt tttaccacct
     4261 ttatcaggtt acgtttcatt tgttccagag gaacattgtc gatttttcgc gcattgctgg
     4321 tggctgggaa tcacctgaat gggtgatttt tgcagtaccg gctatggtgc ggtttgtctt
     4381 gccggatgcg ccgccaggcg cggcttatcc agcctacggg taggccgcat ccggcgttga
     4441 tgggagatgc tctctaaatc caggttgaac aaaacataca caaaaaatat agatcaccgt
     4501 cacatttttg cgttatacag gaagctcgcc attgtgaagg aggtactgct atgacgtcac
     4561 tctctcgtcc gcgcgtggag tttatctcca ccattttgca gaccgtactc aatcttggcc
     4621 tgctgtgcct cggcctgatt ttggttgtct tcctcggcaa agaaacggtg catctggctg
     4681 atgtgctgtt cgcgccagaa caaaccagca aatatgagct ggtagaaggg ctggtggttt
     4741 actttctcta tttcgaattt atcgcgctga ttgtgaagta ctttcagtcc ggttttcact
     4801 tcccgctacg ttactttgtc tatattggga tcaccgccat tgtgcgcttg atcatcgtcg
     4861 atcataaatc gccactggat gtgttgatct actcggcagc gatcctgttg ctggtgatca
     4921 ccctgtggtt atgtaattcg aaacggttga agcgggagta aaaaaaggcg ctccgtggag
     4981 cgccgaataa cagtcacaag ttgggataac gtaagttgag ggtgcagcgg cataacattg
     5041 gcagaacaac atctttaacc tttcacacca cctgccgtca ggccgttgac cagccagcgt
     5101 tgagccagca agaagacgat ggtgatcggt aatgcagaca tcactgcagc ggcggcaaag
     5161 tcaccccaca ggtagttttg cgggttgagg tattgctgca ttcccacggc cagggtgtag
     5221 ctgtttacgt cacgcagtaa cagcgacgcg accggaactt cagtaatggc agcgataaac
     5281 gacaggatga acaccaccgc cagaatcggt actgacagcg gtaacaagac aaggcggaag
     5341 gcttgccacg gtgtcgcacc atccagcgcc gccgcttctt ccagcgaact gtcgatggtt
     5401 tcgaaatagc ctttgatggt ccagacatgc agcgcaatcc cacccagata cgcgaaaatt
     5461 acgccgccgt gagtattcag gccaatgaat ggaatgtact cacccagacg atcaaacaac
     5521 gcatacaacg cgaccagtga aagtactgcc gggaacatct ggaaaatcag cattcctttc
     5581 agcagcgtcg ctttgcctgg aaagcgcata cgggcgaaag cgtaggcgca ggtggtggag
     5641 agcgccacaa tgccaatcgc ggaaatcccg gcgaccttta ccgagttcca cagccacagc
     5701 agtaccggga atggcggtgg cgtaatgcga ccatcagcct gttccacact aaaacctaac
     5761 gccagtttcc agtgatccca ggagatttgc tccgggatca gactaccggt cgcgaagttc
     5821 ccctgacgca gtgagatagc gacgaccatc agcagcggga acataatcgc cgcgataaaa
     5881 agtagcagta gcaggtgagt aataaacaaa cgtgcttttt gcgatttcgg ttggaccatt
     5941 gccatttttg ttatctccct taatcaaact tcattcgcgt ggctttcagg ttcactatcg
     6001 ccagcgcgcc caccagcagg aagatcagcg tggcaattgc tgctgccaga ccgaagtcct
     6061 gacccccgcc gccttcaaaa gcgatgcggt aggtgtagtt aacaagcagg tcggtataac
     6121 cggctggcgt ggtcgtgcca agacgatccg ggccgccgtt ggttaacagt tgaatcagca
     6181 cgaagttgtt aaagttaaag gcaaagctgg cgatcatcag cggcgtcagc ggtttaatca
     6241 gcagcggcag cgtaatctta aagaagttct ggaatggacc tgcgccatcc attgctgagg
     6301 cttcatacaa atcgtccgga atcgctttca gcaagcccat gcagaggatc atcatgtacg
     6361 gataacccag ccaggtgttg acgataatca gcatcgtgcg ggcggtggtc ggatcgctga
     6421 accaggcggg cttcacgcca aacagcgtgc tcaacatcat gttgatctca ccgaagctct
     6481 ggttaaacaa ccctttgaaa atcaagattg aaatgaacga tggcaccgca tagggcagaa
     6541 tcagcaggac gcgatagacc gcttttccgc gcaacgcttc ccactgcacc agacacgcca
     6601 gaaccatgcc gaccgccacc gttaaaaaga cggtgatcag cgagaacacc acggtccaga
     6661 cgaaaatggc gaggaacggt ttctgaatgc cttcgtcggt gaagacgcgg gtaaagtttt
     6721 tccagccagt ggtcacggtg taaccggggc ttagcttttc atcaccccag ttgccgtcgg
     6781 cggtaatgga ctggtaaaag ccaatttggt tattcggacg atatttcacg ccgctctgat
     6841 tattcgtcaa cgtgccgtca ccgtcgagtg tgtagagcgg ctgcgtgcca gaaaactggc
     6901 gcagggagct catcatcact ttgttgccat ctggcagaat ggcggtaatg tcactcagcg
     6961 cctgacgatt ctgggtaatc acgcgcagat tcgcgcgttc gccttcgggc tgggcggtcg
     7021 tttctttcag ttgcagtttt tgctcgccgc caaatttaaa cgcgtcggag aggtaatttt
     7081 tgccggtttc gccgtcgctg agcgccagtt gccactcatc gcccgccggg taaagaccaa
     7141 aattataggt tttgcctgct tgccaggagc gatctaacaa cacttcctgc gcacgttcaa
     7201 aagtcagctg gttagtgctg ctgtagttgg tgaaggcaat ggcgatggtg cagaccaaag
     7261 ggaagaggac gaataatccc attccagcca ttcccgggta aacatagcgc caggcgtagg
     7321 ctttacgatt ggcgaaaata tacagccccg ctgaactcaa tatcagcgtg gtaatggcga
     7381 acaggtattc cccttgtgcg tacattaaaa caacaaggta acccaccagc aggccgagca
     7441 gacctaacac tgaccatttc agcgcgtcgc tttgccacca atgtttcttt ttaatgacat
     7501 ccatggggtt cttcctcatt ccaggacgga taaggctttc acgccttatc cgacaacaac
     7561 tgcctgatgc gacgctgacg cgccttacca ggcctacata cgtttcaatt tcgtaggccg
     7621 gataaggcgt tcacgccgca tccggcattt cacagcatta cttggtgata cgagtctgcg
     7681 cgtctttcag ggcttcatcg acagtctgac gaccgctggc ggcgttgatc accgcagtac
     7741 gcacggcata ccagaaagcg gacatctgcg ggatgttcgg catgatttca cctttctggg
     7801 cgttttccat ggtggcggca atacgtggat ctttcgccaa ctcttcctcg taagacttca
     7861 gcgctacggc acccagcggt ttgtctttat taaccgcttc cagaccttcg tcagtcagca
     7921 gatagttttc gaggaactct ttcgccagct ctttgttcgg actggcggcg ttaatacctg
     7981 cgctcagcac gccaacgaac ggtttggatg gatgaccctt gaaggtcggc agtaccgtta
     8041 caccataatt cactttgctg gtgtcgatgt tggaccatgc ccacgggccg ttgatggtca
     8101 tcgctgtttc gcctttatta aaggcagctt ctgcgatgga gtaatcggtg tctgcattca
     8161 tgtgtttgtt tttaatcagg tcaaccagga aggtcagacc cgctttcgcg ccagcgttat
     8221 ccacgcccac gtctttaatg tcgtacttgc cgttttcata cttgaacgca taacccccgt
     8281 cagcagcaat cagcggccag gtgaagtacg gttcttgcag gttgaacatc agcgcgctct
     8341 tgcctttcgc tttcagttct ttatccagcg ccgggatctc ttcccaggtt tttggcgggt
     8401 tcggcagcag atctttgtta taaatcagcg ataacgcttc aacagcgatc gggtaagcaa
     8461 tcagcttgcc gttgtaacgt acggcatccc aggtaaacgg atacagcttg tcctggaacg
     8521 ctttgtccgg ggtgatttca gccaacaggc cagattgagc gtagccacca aagcggtcgt
     8581 gcgcccagaa gataatgtca gggccatcgc cagttgccgc aacctgaggg aatttctctt
     8641 ccagtttatc cggatgctca acggtgactt taattccggt atctttctcg aatttcttac
     8701 cgacttcagc gagaccgtta tagcctttat cgccgttaat ccagattacc agtttacctt
     8761 cttcgatttt ggcgagagcc gaggcggaaa acatcatcgt cgttaatgcg gataatgcga
     8821 ggatgcgtgc acctgttttt attttcataa tctatggtcc ttgttggtga agtgctcgtg
     8881 aaaacaccta aacggactct agtttcttta tacagcaacc tctttccatc ctccttgccc
     8941 ctacgcccca ccgttgcttt gtgtgatctc tgttacagaa ttggcggtaa tgtggagatg
     9001 cgcacataaa atcgccacga tttttgcaag caacatcacg aaattcctta catgacctcg
     9061 gtttagttca cagaagccgc gttctcatcc tcccgcctcc tcccccataa aaaagccagg
     9121 gggtggagga tttaagccat ctcctgatga cgcatagtca gcccatcatg aatgttgctg
     9181 tcgatgacag gttgttacaa agggagaagg gcatggcgag cgtacagctg caaaatgtaa
     9241 cgaaagcctg gggcgaggtc gtggtatcga aagatatcaa tctcgatatc catgaaggtg
     9301 aattcgtggt gtttgtcgga ccgtctggct gcggtaaatc gactttactg cgcatgattg
     9361 ccgggcttga gacgatcacc agcggcgacc tgttcatcgg tgagaaacgg atgaatgaca
     9421 ctccgccagc agaacgcggc gttggtatgg tgtttcagtc ttacgcgctc tatccccacc
     9481 tgtcagtagc agaaaacatg tcatttggcc tgaaactggc aggcgcaaaa aaagaggtga
     9541 ttaaccaacg cgtcaaccag gtggcggaag tgctgcaact ggcgcatttg ctggatcgca
     9601 aaccgaaagc gctctccggt ggtcagcgtc agcgtgtggc gattggccgt acgctggtgg
     9661 ccgagccaag cgtatttttg ctcgatgaac cgctctccaa cctcgatgct gcactgcgtg
     9721 tgcaaatgcg tatcgaaatc tcccgtctgc ataaacgcct gggccgcaca atgatttacg
     9781 tcacccacga tcaggtcgaa gcgatgacgc tggccgacaa aatcgtggtg ctggacgccg
     9841 gtcgcgtggc gcaggttggg aaaccgctgg agctgtacca ctatccggca gaccgttttg
     9901 tcgccggatt tatcggttcg ccaaagatga acttcctgcc ggtaaaagtg accgccactg
     9961 caatcgatca agtgcaggtg gagctgccga tgccaaatcg tcagcaagtc tggctgccgg
    10021 ttgaaagccg tgatgtccag gttggagcca atatgtcgct gggtattcgc ccggaacatc
    10081 tactgccaag tgatatcgct gacgtcatcc ttgagggtga agttcaggtc gtcgagcaac
    10141 tcggcaacga aacccaaatc catatccaga tcccttccat tcgtcaaaac ctggtgtacc
    10201 gccagaacga cgtggtgttg gtagaagaag gtgccacatt cgctatcggc ctgccgccag
    10261 agcgttgcca tctgttccgt gaggatggca ctgcatgtcg tcgactgcat aaggagccgg
    10321 gcgtttaagc accccacaaa acacacaaag cctgtcacag gtgatgtgaa aaaagaaaag
    10381 caatgactca ggagatagaa tgatgattac tctgcgcaaa cttcctctgg cggttgccgt
    10441 cgcagcgggc gtaatgtctg ctcaggcaat ggctgttgat ttccacggct atgcacgttc
    10501 cggtattggc tggacaggta gcggcggtga acaacagtgt ttccagacta ccggtgctca
    10561 aagtaaatac cgtcttggca acgaatgtga aacttatgct gaattaaaat tgggtcagga
    10621 agtgtggaaa gaaggcgata agagcttcta tttcgacact aacgtggcct attccgtcgc
    10681 acaacagaat gactgggaag ctaccgatcc ggccttccgt gaagcaaacg tgcagggtaa
    10741 aaacctgatc gaatggctgc caggctccac catctgggca ggtaagcgct tctaccaacg
    10801 tcatgacgtt catatgatcg acttctacta ctgggatatt tctggtcctg gtgccggtct
    10861 ggaaaacatc gatgttggct tcggtaaact ctctctggca gcaacccgct cctctgaagc
    10921 tggtggttct tcctctttcg ccagcaacaa tatttatgac tataccaacg aaaccgcgaa
    10981 cgacgttttc gacgtgcgtt tagcgcagat ggaaatcaac ccgggcggca cattagaact
    11041 gggtgtcgac tacggtcgtg ccaacctgcg tgataactat cgtctggttg atggcgcatc
    11101 gaaagacggt tggttattca ctgctgaaca tactcagagt gtcctgaagg gctttaacaa
    11161 gtttgttgtt cagtacgcta ctgactcgat gacctcgcag ggtaaaggtc tgtcgcaggg
    11221 ttctggcgtc gcgtttgata acgaaaaatt tgcctacaat atcaacaaca acggtcacat
    11281 gctgcgtatc ctcgaccacg gtgcgatctc catgggcgat aactgggaca tgatgtacgt
    11341 gggtatgtac caggatatca actgggataa cgacaacggc accaagtggt ggaccgtcgg
    11401 tattcgcccg atgtacaagt ggacgccaat catgagcacc gtgatggaaa tcggctacga
    11461 caacgtcgaa tcccagcgca ccggcgacaa gaacaatcag tacaaaatta cccttgcaca
    11521 acaatggcag gctggcgaca gcatctggtc acgcccggct attcgtgtct tcgcaaccta
    11581 cgccaagtgg gatgagaaat ggggttatga ctacaccggt aacgccaata ccaacactaa
    11641 cttcggcaaa gccgttcctg ctgatttcaa cggcggcagc ttcggccgtg gtgacagcga
    11701 cgagtggacc ttcggtgccc agatggaaat ctggtggtaa tagcaaaaac tgggccggat
    11761 aaggtgcttg cgccgcatcc ggcataaaaa caggttgtca ttatctgaaa ggggcgcaag
    11821 cccctctgat tatcgggttt agcgcgctat tgcctggcta ccgctgagct ccagattttg
    11881 aggtgaaaac aatgaaaatg aataaaagtc tcatcgccct ctgtttatca gcagggttgc
    11941 tggcaagcgc gcctggaatc agccttgccg atgttaacta cgtaccgcaa aacaccagcg
    12001 acgcgccagc cattccatct gccgcgctgc aacaactcac ctggacaccg gtcgatcaat
    12061 ctaaaaccca gaccacccaa ctggcgactg gcggccaaca actgaacgtt cccggcatca
    12121 gtggtccggt tgctgcgtac agcgtcccgg caaacattgg cgaactgacc ctaacgctga
    12181 ccagcgaagt gaacaaacaa accagcgtat ttgcgccgaa cgtgctgatt cttgatcaga
    12241 atatgacccc atcagccttc ttccccagca gttatttcac ctaccaggaa ccaggcgtga
    12301 tgagtgcaga tcgtctggaa ggcgttatgc gcctgacacc ggcgttgggg cagcaaaaac
    12361 tttatgttct ggtctttacc acggaaaaag acctccagca gacgacccaa ctgctcgacc
    12421 cggctaaagc ctatgccaaa ggcgtcggta actcgatccc ggatattcct gacccggttg
    12481 ctcgtcatac caccgatggc ttactgaaac tgaaagtcaa aacgaactcc agttccagcg
    12541 tgttggtcgg gccactattt ggttcttccg ctccagctcc ggttacggta ggcaacacgg
    12601 cggcaccagc tgtggctgca cccgctccgg caccggtgaa gaaaagcgag ccgatgctca
    12661 acgacacgga aagttatttt aataccgcga tcaaaaacgc tgtcgcgaaa ggtgatgttg
    12721 ataaggcgtt aaaactgctt gatgaagctg aacgcctggg gtcaacatct gcccgttcca
    12781 cctttatcag cagtgtaaaa ggcaaggggt aattacgccc cacagtgctg attttgcaac
    12841 aactggtgcg tctcctggcg cacctttttt tatgcttcat tccagggata tgagcgattt
    12901 tttatagtac ttcacttctt cttcactaag aatatccatt atctcaatgc attatcagag
    12961 attcttttcc tttcgccggt agtgtctgga cgctcaggct actttttcca gttatttcat
    13021 ttctgtaatg cagaggtttt atgttaggtc atatctcaaa gtttgacggc aataactctt
    13081 tgataaaaca tgatgtggtg caaggaaata ataaattaga tcttgattta ctacgtaatt
    13141 ttaatggggt gccaggttta aatagagata actttattta tatcagtga
//
LOCUS       DACBVI010000122        13118 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.122, whole genome shotgun sequence.
ACCESSION   DACBVI010000122 DACBVI010000000
VERSION     DACBVI010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.122"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..502
                     /locus_tag="IU325_17130"
     CDS             <1..502
                     /locus_tag="IU325_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP5810723.1"
                     /translation="PYVNLFRADLSFSKLDLINFEHADLSRVNLNKATLQNINLIDSK
                     LFFTRLTNTFLEMVICTGSNMANVNFNNANLSNCHFNCSILTKAWMFNTRLYRVNFDE
                     ANVQGMGISILREEENIPINSDTLITLQKFFEEDCTSHTGMSQTEDNIHAVAMKITAD
                     IMRDAD"
     gene            complement(656..2305)
                     /locus_tag="IU325_17135"
     CDS             complement(656..2305)
                     /locus_tag="IU325_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HAP5810724.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2456..3805)
                     /locus_tag="IU325_17140"
     CDS             complement(2456..3805)
                     /locus_tag="IU325_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP5810725.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4351..4815)
                     /gene="soxR"
                     /locus_tag="IU325_17145"
     CDS             complement(4351..4815)
                     /gene="soxR"
                     /locus_tag="IU325_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HAP5810726.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLISSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIREAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            4901..5224
                     /gene="soxS"
                     /locus_tag="IU325_17150"
     CDS             4901..5224
                     /gene="soxS"
                     /locus_tag="IU325_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HAP5810727.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5227..6813)
                     /gene="pdeC"
                     /locus_tag="IU325_17155"
     CDS             complement(5227..6813)
                     /gene="pdeC"
                     /locus_tag="IU325_17155"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HAP5810728.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNSQLQT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVVVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIHLNDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLIAHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIGQV
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARTNQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            7243..7524
                     /locus_tag="IU325_17160"
     CDS             7243..7524
                     /locus_tag="IU325_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810729.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(7623..8159)
                     /locus_tag="IU325_17165"
     CDS             complement(7623..8159)
                     /locus_tag="IU325_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HAP5810730.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            8414..11236
                     /gene="uvrA"
                     /locus_tag="IU325_17170"
     CDS             8414..11236
                     /gene="uvrA"
                     /locus_tag="IU325_17170"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP5810731.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(11271..11627)
                     /locus_tag="IU325_17175"
     CDS             complement(11271..11627)
                     /locus_tag="IU325_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAP5810732.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(11631..12047)
                     /locus_tag="IU325_17180"
     CDS             complement(11631..12047)
                     /locus_tag="IU325_17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAP5810733.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(12158..12871)
                     /locus_tag="IU325_17185"
     CDS             complement(12158..12871)
                     /locus_tag="IU325_17185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B acid phosphatase"
                     /protein_id="HAP5810734.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         3404 a         3429 c         3199 g         3086 t
ORIGIN      
        1 gccgtatgtt aatttattta gagccgatct tagtttctct aaactcgatt taattaactt
       61 tgaacatgct gatctgtctc gcgtcaatct gaacaaagca actctccaga atataaactt
      121 aattgatagc aaactctttt ttacgcggct gacaaataca ttccttgaaa tggttatatg
      181 taccggctct aatatggcta atgttaattt taataatgcc aatttaagca attgccattt
      241 caactgttct attttaacaa aagcctggat gtttaatacc cgtctctatc gggttaattt
      301 cgatgaggct aacgtgcagg gaatgggcat ttccattctc cgtgaggagg aaaatatccc
      361 cattaatagt gataccctga taacactaca gaaattcttt gaagaagatt gtacctctca
      421 tactggcatg tcacaaactg aggataatat tcatgcagtc gctatgaaga ttactgcaga
      481 tattatgcga gatgcagatt gaggttaagc cgaagaacag ccaatgaatt gactttaaaa
      541 gagagataac ttatttgttt tagaatcatg tatgttagcc cgagctaacc tgttaaaggt
      601 gcaaaagatt gcaccttttt caaattacct cgggcctggt tacgtcgctt tcggcttact
      661 gattttcctc aatcaacaac gcttcaagca aatcgagatc gtgcagtaat ttttgcagcg
      721 tttcattgct gatctcccgc gtggcgcgca ggtggtacag ttcagcacgt tcagaacgca
      781 atgccgccag acggaagcga cgctcaaggt tctcttcctg cacggaactt tcaacgtcgt
      841 tacgtccatc ggcgcgacga cgcaggttac caatgacgcg agaactgacc tccgtgagca
      901 gctggttatc gatattctct tcggtatccg ccgccagacg ctcctccatt ttctggatcg
      961 ccacaatcgc cacttctgcc gttgccgctc gcgcaatacg ttcctctttc aattgttgcg
     1021 aatggtccgc gacttcaatg tgttgtagca gaataggcaa catcaccacg ccgacaaaca
     1081 gcgagaagag aatgacacca gccgccagga ataccagctc atagcgcgcc gggaagacgt
     1141 taccatccgg caagagcagc gggatagaga gcacaccagc cagagtgatc gccccacgca
     1201 ccccggcgaa agacgcgatc aggatttctc gtgtggtcca cgaaccaaac tccatcggct
     1261 tctttttcag gaagcggttg ctgaactttt tcatcgtcca cagccagccg aaacggacca
     1321 gcatcagcgc cgcataaatc agaatgatat cggtaaacag catccagatt tcgacgttag
     1381 ggtcgatttc tgccgccatc agcgacgtct ccagaatacc cggcagctgc agacctaaca
     1441 gcaggaatac catgccgtta aagacaaatt ccagcatcgc ccaggtgctg tttgcgcgca
     1501 ggcgcattgc cagcggcgca cggcgcatca caccggagcg ggtgatggtc atcccggcag
     1561 caaccgcagc gaggatgccg gaaacgccaa tatgttcggc aatcagataa gaagcgaatg
     1621 gcagcaggaa cagcaggacg atctgcgtcg cgggttcatc accgccccag cggctgagga
     1681 atcgcagcga gcgaccgtac agccagctca ccacaaaacc ggcgagaata ccgccaatgg
     1741 ctactttcat aaattcgacc gtcgcaccgc caacggtgaa gatcatcgtc cccatcgcca
     1801 ctgctacggc aaacttcaac gacaccaggc cagaggcgtc attcatcaac gcttcgccct
     1861 gcaaaatgcc catgattttt ttcgggatgc gcccttcgcc aacaatcccg gagagcgcca
     1921 cagcatcggt cggagaaagc accgccgcca gcgcaaaggc ggggatcagc ggaatgcccg
     1981 gcaccaccca gtaaataagg aagccaatgc cgaccacggt gaccaccacc agcgccagcg
     2041 cgaggccgaa aatctctcga ccatgttcga gaaattcacg ggtcggcgtt ttccagccat
     2101 cagcgaacag caacggcggg ataaacaaga ctaaaaagag ttcaggatca aactccacat
     2161 gcaaaccaaa cgtcggccac gccagtagcg caccgatggc gatttgcata agcggaagcg
     2221 ggatctgaaa gggcatgaca cgagtgacca ccccggacag cgagaccacg agggtcatta
     2281 tcagtatggt gaagaagatt tccatgcgtt ccctgtttgc gatgtttttt atgatctgag
     2341 aaagacgtta tgcctgaatt ctcatttttt cagaatatcg caaaagctca cacaatgtgc
     2401 ggagaaaata aaaacgggca gccattgcca cccgttttca gaatcatatg aaagattaga
     2461 tagcccagcc acccgcatag aaggtcacca gcgccacagc gataacgacc gtaccgatgt
     2521 tcaacttgcg ccattcgccg gaaaccagac gaccaatcac cagagtcgcg aagccgatca
     2581 tgatgcctgt tacgatgtta caggtcagca cgatgaatac cgccgtaacc agacccgcca
     2641 tcgcatcaac aaaatcagca aagtcgattt tcgccacgtt actcagcatc agcaggccaa
     2701 cgtacatcag cgccggagcc gttgcatacc ccggaacgag gtaagagagc ggagagagga
     2761 acagaatcag caggaacagc acgccaacgg tgatagccgt caacccggtt ttaccgcccg
     2821 ccgccgtacc cgccgcagac tcgatgtata ccgctgccgg agctgcaccc accaggccag
     2881 agaaaacgct gctcatggag tcggtggtca gtgctttccc accgtcaatg atctgcccat
     2941 ctttatccag caggttcgcc tgaccggcga cggcgcggat agttccggtg gcatcaaata
     3001 ctgccgtcat caccagcgcc agaacgcttg gcaggactac aggattcagc gcgcccataa
     3061 tgtccaggct accaatcagt gaattgccgt tttcatcgct caatgaaggc atggcgaaaa
     3121 cgccggagaa atggacgtta ggatcgaaga tcaaaccgac aattgagata ccgataatgg
     3181 tcagcagaat gccaccaggg actttcagtt tttccaggcc gatgatcacc gccagaccta
     3241 ccagtgacat aatcaccggg aaggtcgcga aatcacccag cgcaacgggc agaccatcaa
     3301 gcgggttttt aatcaccaga ccgacaccat tagcggcaat gagcagcagg aacagaccga
     3361 taccgatccc cgtgccgtgc gccacaccgt gcggcaagtt gcgcaaaatc cagctacgga
     3421 tacccgtggc agaaattacc gtaaacagta cacccatcag gaacacagca cccagcgcga
     3481 caggtacgct aatatgttgc cccagcacca gactgaatgc ggtaaacgcc gtcagggaga
     3541 tggcgcaacc aatcgccaac ggcagattag cccacagacc catcacgata gaaccgagtc
     3601 cggcaaccag acaggtcgca acgaaaactg ccgcaggcgg gaagcctgct ttacccaaca
     3661 tgcctggaac gacgataacc gagtagacca tcgccagaaa tgttgttaac ccggcaacca
     3721 cttcctgacg gacagtgctt ccacgttgtg aaattttaaa ccaggcgtcg agtgaaccgc
     3781 cggtacgcgc tgatggcgta gacataagaa aacatcccct gagagtttaa ttttcgtcaa
     3841 gatgcgtggc ggacaatccc ctgccagcga aacgttatct tcctgctcca ggtttgcgac
     3901 aaattggtgc gtcgcaaaaa agcaaacgtt tagctccgcg catacaaaac gggtggtaaa
     3961 aaaagcaaac gattatccgg cgatcatagc ggctttttca accgaagttt gctgcttttt
     4021 tctcttttat tgtcttgacg atgaaaaacc aggcaattga tgcgcaaacg ttatcgtcga
     4081 tgcgcaagat tgcagcgtta tcgagccata taccgttcag gattgcgata aaaaatcgtc
     4141 ttagatgaca aaaatattgc ggctaaaaag tgtactttta ctctggcgaa gaaacgccgg
     4201 tgacatacaa ttaaagcatc aacaccaacc ggaacctcca ccacgtgctc gaatgaggtg
     4261 tgttgacgtc gggggaaacc ctcctgtgta ccagcgggat agagagaaag acaaagaccg
     4321 gaaaacaaac taaagcgccc ttgtggcgct ttagttttgt tcatcttcca gcaagcgtgc
     4381 gcctgtgcct tcttctccta agcggtcgcc cgggttacgc aatgggcaat cactgcgcga
     4441 aaggcagcca caaccaatac atccgtccag ctcgtcacgc agcgccacta aggtatgaat
     4501 gcgccgatcc aactcttctc gccattggga cgaaagctgt ttccactctt tcgcacttaa
     4561 tgtatgccct tcgggcaaca cgccgaatgc ttcacgaatg gtcgccagcg gaatgccaat
     4621 acgctgagca attttgatga ttgcgacata tcgcaacaca tcacgtttat atcgccgctg
     4681 attgccacta ttacggatac tgctaatcaa ccctttgctt tcatagaaat gcagcgccga
     4741 taccgccaca ccgctgcgtt ttgccacttc gccgggggtt agcagcgctt taatgcgggg
     4801 taatttcttt tccataaatc gctttacctc aagttaactt gaggaattat actccccaac
     4861 agatgaatta acgaactgaa cactgaaaag aggcagattt atgtcccatc agaaaattat
     4921 tcaggatctt atcgcatgga ttgacgagca tattgaccag ccgcttaaca ttgacgtcgt
     4981 cgcaaaaaaa tcaggctatt caaagtggta cttacagcgg atgttccgca cggtgacgca
     5041 tcagacactt ggtgattaca ttcgccaacg ccgcctgtta ctggccgccg ttgagttgcg
     5101 caccaccgag cgtccgattt ttgatatcgc aatggacctg ggttatgtct ctcagcagac
     5161 cttctcccgc gttttccgtc ggcagtttga tcgcactccc agcgattatc gccaccgcct
     5221 gtagttttat tgcccgcgcg ttaactcccg cgcgggtaat tgctccatcc attgcataaa
     5281 cacctgcggc ggcatcgcct tcgcaaagaa ccatccctga caatagcgca cgccgcgttt
     5341 gcgcagccag ttaacctgct cctcagtttc gacgccttca gcgatcgttt ttaaccccag
     5401 actgtgcgcc agctcgatga tgtgttccgc aatcaaatga ctggttttgt gggtggtcag
     5461 cgtctcaaca aacgatttat cgattttcaa aatatcgaca ttcaatgatt taaggttatg
     5521 caagttagag tagccaatac caaaatcatc aattgccact tcgtaaccag cctggcggaa
     5581 cgccagaata attggtgtca ttttgtcgac atcaagaaat gcatgttcag tcacttcaaa
     5641 tttaatttgc tgtggacgca ccgcgtattg ctctgttttc tgattggttc gcgctatcaa
     5701 ccgtgacgta tgaaaatcgg aggccgacag gttaatagaa acatagcgat cggcatgtgt
     5761 tgccaggtac gcgcccagat cgcggaagac attatcaata acataatcag ttacctgtcc
     5821 gatcatcccc tccttttctg ccagcggaat aaactctgcc ggattcatta cttgcccctg
     5881 ctcaccaggc caacgtaaca atgcttcagc gccgatacat ttttctgttt tgatatcgat
     5941 tattggctgg taataaaggc aaagttgatg tttttcgagg gcgcgttgca atttgcgttt
     6001 gggagacaaa tagttttgcc gaatacgtag ccagagtaat aacagaacaa gactccccaa
     6061 aataccggcg ggtaacgcaa aaataagatg attataaaaa tgcgctataa gacgttgata
     6121 tgaagtcgca acaatggctg caattgggcg ttttgtcgaa taaactgtcg catataaata
     6181 gccatttctt tgtacggtta aatcattcaa atgaatcagt ggagaaaacg ttgctgccga
     6241 ggcctcgttg cttaacgaga aaaaggtttt cgttaccgta tcatacacac cccattgcaa
     6301 tgtcggatca tcagacatca cttcactcca gaagagaggg ttgatgacca ccacataatt
     6361 tccccgctgc atataggtca ttttatagcc agagaaaaaa ggcgtatcgc ggtaataata
     6421 gatagaaacg ttaggttcac gcttataatc ggccggtgca atcgtatagc cgtttacagg
     6481 cgctatcagc gatgagcata aaaaatggtt atcacgggca tagatcaatt cattaatata
     6541 aagatagcca cgaataatat tcaacattcg cttttgatgg gctggagtgc aaacttgccc
     6601 ctgatagcgt tctgctgcat cgctcactaa atctgcctgg cgaatgacca gctcggattt
     6661 atccagcgcc agctgagcaa aagtttggag ctgactattc acttctgatt ttgcccaaag
     6721 gaaggcaatc catagcgaca gtatgatggg aaagagaact aaaaagataa tgcccggcaa
     6781 cgccagtaat tggtgtcgtg cacgatgact cattgctatc aatttcctgc ctgtttatac
     6841 ccggtcattg caaggcgaga accctggtta tcgacgtctt tagataaatc ttacggtata
     6901 aatagcactt ttgcctgatt tgaaattgat acctttttaa tcgtcaccta aataaaaata
     6961 attcattgat attcaaaact aatttcctac aggaatgcag aggcggtggg aagatatccc
     7021 cttaatgccg ctgaaaaaac acataatctg tacggcaata aaagtcgcgg cctaatctgg
     7081 ctaacaaaca atcaattgtg atatagttca caaaattaat gaaacaaaca gagtgtttct
     7141 tttttgtgtt ctgcaagccc cgcgatagcc agattgtatc acgcctcccg tgaacaacgc
     7201 cgcgctgtcc gaggcatcgg ctcttttcag aggaattgtt tgatggctac cctcaccact
     7261 ggcgtggttc ttcttcgctg gcaacttctt agtgccgtaa tgatgtttct ggccagcaca
     7321 ctcaacatcc gttttcgtcg gtctgattat gtcgggcttg cagtgatcag cagcggtctg
     7381 ggcgtagttt ccgcctgctg gttcgcaatg gggttgcttg gcatcacaat ggcggatatc
     7441 accgccatct ggcacaacat cgagtcggtg atgatagaag agatgaatca gacaccgcca
     7501 caatggccaa tgattttgac ttgatatgta gaagcctcca aaacggaggc ttctttttta
     7561 cggctggcag atgttttaat catccacctt aaaacaatat aacctattgt tttaatgaaa
     7621 aatcagaacg gaatatcgtc atcaaagtcc atcggcggct cattagacgg tgctgccgga
     7681 gcagactgct gcgggcgaga ctgcgcgccg ccgctgaact gattgccgcc ctgcggctgc
     7741 tgaggctgac cccaaccgcc ctgcggctgg ccaccaccga tattgccacc tgcaggagcg
     7801 ccaccaccct gacgaccacc cagcatctgc atggtgccgc caacgttcac caccacttca
     7861 gtggtgtagc gatcctgacc ggattgatcg gtccatttac gggtacgcag ctgaccttcg
     7921 atataaacct gagaaccttt acgcagatat tcgctggcca cttctgccag tttgccgaac
     7981 agcacaacgc ggtgccattc agtctgctct ttcatctcgc cggtcgcttt atcacgccag
     8041 gattcggaag tagccagcgt aatgttggca acagcgccac catttggcat gtagcgtact
     8101 tccgggtcct gacccagatt accaacgaga ataaccttgt ttacgcctct gctggccatg
     8161 ttcgtgtctc ctgaaaaaaa atcgttctga ataagtgtaa acgcgcgatt gtaccattac
     8221 caatagcgct tttactatgt tgtgacctcg gttccgggaa acaaacctgg ccagacattg
     8281 ttacacaaca ctccgggtaa tgcattccaa tactgtatat tcattcaggt caatttgtgt
     8341 cataattaac cgtttgtgat cgccggtagc accatgccac cgggcaaaaa agcgtttaat
     8401 ccgggaaagg tgaatggata agatcgaagt tcggggcgcc cgcacccata atctcaaaaa
     8461 catcaacctc gttatccccc gcgacaaact tattgtcgtg accgggcttt cgggttctgg
     8521 caaatcctcg ctcgctttcg acaccttata tgccgaaggg cagcgccgtt acgttgaatc
     8581 cctttccgcc tacgcgcggc agtttctgtc actgatggaa aagccggacg tcgatcatat
     8641 tgaggggctt tctcctgcca tctcaattga gcagaaatcg acgtctcata acccgcgttc
     8701 tacggtgggg acaatcaccg aaatccacga ctatttgcgt ttgttgttcg cccgcgtcgg
     8761 tgagccgcgc tgcccggacc acgatgtacc gctggcggcg caaaccgtca gtcagatggt
     8821 ggataacgtg ttgtcccagc cggaaggtaa acgcctgatg ctgctcgcac caatcattaa
     8881 agagcgcaaa ggcgaacaca ccaaaacgct ggaaaatctg gcaagccagg gttacatccg
     8941 tgctcgtatt gatggcgaag tctgcgatct ttccgatccg ccgaaactgg aactgcaaaa
     9001 gaaacatacc attgaagtgg tggttgatcg cttcaaggtg cgtgacgatc tcacccaacg
     9061 tctggcagag tcgtttgaaa ccgcgctgga gctttccggt ggtaccgcgg tagttgccga
     9121 tatggacgat ccaaaagcgg aagagctgct attttccgcc aacttcgcct gcccaatttg
     9181 cggctatagt atgcgtgaac tggaaccacg cctgttttcg tttaacaacc cggcgggggc
     9241 ctgcccaacc tgtgacggcc ttggcgtgca gcaatatttc gatcctgacc gcgtgatcca
     9301 gaatccggaa ctgtcgctgg ctggcggtgc gatccgaggc tgggatcgcc gcaacttcta
     9361 ttacttccag atgctgaaat cgctggcaga tcactataag ttcgacgtcg aagcgccgtg
     9421 gggcagcctg agcgcgaacg tgcataaagt ggtgttgtac ggttctggca aagaaaacat
     9481 tgaattcaaa tacatgaacg atcgtggcga tacctccatt cgtcgtcatc cgttcgaagg
     9541 cgtgctgcac aatatggagc gccgttataa agagacggaa tccagtgcgg tacgtgaaga
     9601 attagccaag tttatcagca atcgcccgtg cgccagctgc gaaggaaccc gtctgcgtcg
     9661 ggaagcgcgc catgtgtatg tcgagaatac gccgctgcct gctatctccg acatgagcat
     9721 cggtcatgcg atggaattct tcaacaatct caaactcgca ggtcagcgag cgaagattgc
     9781 ggaaaaaatt cttaaagaga ttggcgatcg cctgaaattc ctcgttaacg tcggcctgaa
     9841 ttacctgaca ctttcccgct cggcagagac actttctggc ggtgaagccc agcgtatccg
     9901 tctggcgagc cagattggtg cgggcctggt tggcgttatg tacgtgctcg atgagccgtc
     9961 tatcggcctg caccagcgcg ataacgagcg cctgttgggt acgcttatcc atctgcgcga
    10021 tctcggtaat accgtgattg tggtggagca cgacgaagac gcgattcgcg ccgctgacca
    10081 tgtgatcgac attggtcctg gtgcgggtgt tcacggcggt gaagtggtcg cggaaggtcc
    10141 actggaagcg attatggcgg tgccggagtc gttgaccggg cagtacatga gcggtaaacg
    10201 caagattgaa gtgccgaaga aacgcgttcc ggcgaatccg gaaaaagtgc tgaagctgac
    10261 aggcgcacgc ggtaataacc tgaaagacgt gacgctgacg ctgccagtcg gtctgtttac
    10321 ctgcatcaca ggggtttcag gttccggtaa atcgacactg attaacgaca cactgttccc
    10381 gattgcccaa cgccagttga atggggcgac catcgccgaa ccggcaccgt atcgcgatat
    10441 tcagggactg gagcatttcg ataaagtgat cgatatcgac caaagcccaa ttggtcgtac
    10501 tccgcgttct aacccggcga cctataccgg cgtgtttaca cctgtgcgcg aactgtttgc
    10561 gggcgtaccg gaatcccgtg cgcgcggcta tacgccggga cgtttcagct ttaacgtccg
    10621 tggcgggcgc tgcgaagcct gtcagggcga tggcgtgatc aaagtggaga tgcacttcct
    10681 gccggatatc tacgtgccgt gcgaccagtg caaaggtaaa cgctataacc gcgaaacgct
    10741 ggaaattaag tacaaaggca aaaccatcca cgaagtgctg gatatgacca tcgaagaggc
    10801 gcgtgagttc tttgatgcgg tgccagctct ggcgcgtaag ctgcaaacgt tgatggacgt
    10861 tggcctgacg tacattcgcc tcgggcagtc cgcaaccacg ctttctggtg gtgaagccca
    10921 gcgcgtgaag ctggcgcgtg agctgtcaaa acgcggcacc gggcagacgc tgtatattct
    10981 tgatgagccg accaccggtc tgcacttcgc cgatattcag caactgctcg acgtactgca
    11041 taaactgcgc gatcagggca acaccattgt ggtgattgag cacaatctcg acgtgatcaa
    11101 aaccgctgac tggattgtcg acctgggacc agaaggcggc agtggcggcg gcgaaatcct
    11161 cgtctccggt acgccagaaa ccgtcgcgga gtgcgaagct tcgcatacgg cacgcttcct
    11221 caagccgatg ctgtaatcgt taaggccgct ttccgagcgg ccttttcctt tcagagttgc
    11281 accagcaatt tacgtttttc ttccggcagc aaattcaccg cctgctgata agacgcatcc
    11341 accagataat agatttgcga atctggcagc gaaccgtcga gatacacggt gctccagtgc
    11401 gctttattca gatggcggct tggacgcaca tcgctgtgct gctgacgtag cagctccgcc
    11461 agctccgggc tggttttcag cgaaaccgcc gggcgatttt ctacttcttt caccatcgca
    11521 aacagtacat cttccacttt gatctgcgtc gctttccagt cgttatgcac gctctgttct
    11581 gcgcctggtt ttgccatgca atattgtagc aactccgaaa tggtcatttt ttactcccct
    11641 tgtagtgtcg cgataatgcg acgcgatccg ccgtggatgc gatgttcccc cagccagatg
    11701 ccttgccagg tgccggtctg aatacgccct ttatgcaccg gcaatacaag cgatgttccc
    11761 agcattgagg atttgatatg agaaggcata tcgtctgctc cctcatagtc atgctcataa
    11821 tttccgttgt cgggaacggt gcggaggaaa aaacgctcca tgtcgtggcg tacggtggga
    11881 tcgcagttct cattaagtgt cagagaggcg gaggtatgtt gcagcaacag atgcagtaaa
    11941 ccgatgttaa cgcgcggcat atcagccagc tgattcagaa tttcatccgt taccagatga
    12001 aacccacgag atttggcgct aagcgtgagc gtcttttgat accacatgtg ctgctccttg
    12061 ataaaactct cttaatcagt ttgcagcaag acagagaaag gataaaggtg tgattaaaaa
    12121 aacagcattc gggagagtgt cacgctctcc cgctctgtca gtattctgaa ttgacgatca
    12181 cctcttcacc aaacgcaccc gcttgtggca agggtttgta ggtagagttg gaggcgcgca
    12241 gaatgcggat accacgagcg ccgacatcgc gtgcggcggt aatatcgtta tcggaatcgc
    12301 cataaaaaat tcggatattt ttatcctgca gccattgcga ttttgtattt tgccctggtt
    12361 tatcacccgc aaagatcacc ggattcatat tggtggcagg aatatgaaaa ttatccgcca
    12421 gcgtttttga aaccgtttct gttttcgtcg ggctacgacc agtcacaaag aagatcgcgt
    12481 caccgcggcg tacatgcata tcaatcagct ggcgagcgac ctcttttgga atgctgaatt
    12541 catcccagcc attgttcatt ttttcccaga acacaggatt tttcagataa tcttcgcttt
    12601 ctggcgagaa ggtttttttg ccgcgccaga agcccggact ggaaaaaagt accgtgtcat
    12661 cgatatcaaa ccccaccgcc attggcggac gccctgcgag gctattttca atttgtgcga
    12721 ccgaaaccca atgaattggt gcctgttcag caagcctggc aacgttagta ccagggttaa
    12781 gcggcgaagg agatgaggcc agggcaacag cggaactgtt tagagcgaac aataagcaaa
    12841 cggcactgat tgcctgtgtg atcttgcgca tatttttccc taaatagtca gtatgttgaa
    12901 actttttatt gtgagatttg ttgcaaaaac catctgacca taacgccagc agcaggggaa
    12961 aggaaggatt atctgcggtt tttgtgatga aaagcaggat gattatattt aacccagtaa
    13021 atacgttcct gtcgtttttt tgtaaaaaaa tgtttattcc tcaatgaagg atacattaat
    13081 tttacgttta ttatgatgaa tcctctgcaa atgagcga
//
LOCUS       DACBVI010000123        12948 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.123, whole genome shotgun sequence.
ACCESSION   DACBVI010000123 DACBVI010000000
VERSION     DACBVI010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.123"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..212)
                     /locus_tag="IU325_17190"
     CDS             complement(<1..212)
                     /locus_tag="IU325_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810735.1"
                     /translation="MKNLDNIIWHDGVLVETRMECHHTEYNFILTAELYVDEFTAGRE
                     RKRFEFTGVEHLSQVMDIAELIDNQNA"
     gene            complement(497..4399)
                     /gene="hrpA"
                     /locus_tag="IU325_17195"
     CDS             complement(497..4399)
                     /gene="hrpA"
                     /locus_tag="IU325_17195"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HAP5810736.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRQRFLRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKKDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKTRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTSNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            4600..5205
                     /locus_tag="IU325_17200"
     CDS             4600..5205
                     /locus_tag="IU325_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP5810737.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAE"
     gene            complement(5319..6179)
                     /gene="pdxI"
                     /locus_tag="IU325_17205"
     CDS             complement(5319..6179)
                     /gene="pdxI"
                     /locus_tag="IU325_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HAP5810738.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALHPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKITEIVCVQNEYNIAHRADDAMIDDLARVGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(6387..12878)
                     /locus_tag="IU325_17210"
     CDS             complement(6387..12878)
                     /locus_tag="IU325_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HAP5810739.1"
                     /translation="MQRKTLLSACIALALSGQGWAADITEVETTTGEKKNTNVTCPAD
                     PGKLSPEELKRLPSECTPLVEQNLMPWLATGAAALITTLAVVELNDDDDHHHRNNSPL
                     PPTPPDDDSDDTPVPPTPGGDEIIPDVPDDTPTPPKPISFNNDVILDKTEKTLTIRDS
                     VFTYTENADGTISLQDNSGRKATINLWQIDEANNTVALEGVSADGTTKWRYNHNGELV
                     ITGDNATVNNNGKTTVDGKDSTGTEINGNNGKVIQDGDLDVSGGGHGIDITGDSATVD
                     NKGTMTVTDPESIGIQIDGDKAVVNNEGDSTISNGGTGTQINDDDATANNNGKTTVDG
                     KDSTGTEINGNNGKVIQDGDLDVSGGGHGIDITGDSATVDNKGTMTVTDPESMGIQID
                     GDKAIVNNEGESTITNGGTGTQINGDDATANNNGKTTVDGKDSTGTEINGNNGKVIQG
                     GDLDVSGGGHGIDITGDSATVDNKGTMTVTDPESIGIQIDGDKAVVNNEGDSSISNGG
                     TGTQINGDDATANNSGKTTVDGKDSTGTEINGNNGKVIQDGDLDVSGGGHGIDITGDS
                     ATVDNKGTMTVTDPESIGIQIDGDKAIVNNEGDSTISNGGTGTQINGDDATANNNGKT
                     TVDGKDSTGTEINGNNGKVIQDGDLDVSGGGHGIDITGDSATVDNKGTMTVTDPESMG
                     IQIDGDKAIVNNEGESTITNGGTGTQINGDDATANNNGKTTVDGKDSTGTEINGNNGK
                     VIQDGDLDVSGGGHGIDISGDNAAVDNKGNMTVTDADSIGVLINGDNATLANTGDVDV
                     SNSATGFAITSDEGKISLAGSMQVGDFSTGLTLTGDNNSLTMAMQDLNVVGQKATGIN
                     VSGDANTIDITGNILVDKDQSADNAAEYFYDASVGVNVSGHNNNVTLDGKLTLVADGE
                     TTTRDVSNFDGSQENISGLIVSGDGNNINLNGGIAFVGEINALTDGSAIANKRTFFGN
                     TPLIGIDGESKVYLNGDSTISGDLQLGYASLIQLKNKAAIEIGSEATFNMRDIENYDH
                     YYAQTPQIIKAESSSEVINNGNVDIWNISFAGIFGENTTGINNGNITLSLYDYASTNT
                     PAPEPDNTAFLTSNGGSAVNKGVITSKVMEQHSVVNMAALTGSTDQRVFNNSVASMMG
                     MEAYNKGSVLNAEDAVIDMYGRGSIGMLAIDNSTADNAGNITVDTLWIDDNDTTSLHT
                     DLPGATAKDYGVGMATGTDTGGGARNNAIATNLEGGVITVYNAGAGMAAYGNSNMVIN
                     QGIINLEKNADYDANLGSNTLVGMAVYKGATAINDQTGVININVDTGQAFYNDGTGII
                     LNYGEINLNGAEIDSTDSHYGAPAENLELLSELSASGENITKTVIRDGFVTIKPLANY
                     GTEILNGDVDANLWLYNEDKASLTVNGDLNIVQGLENSGSMDADKLTANASVYNRASG
                     SMTTELLMLKGGSAFFNEGSFSGVISGDSYKQNVVNTGEMTTVTDGSALINGSFVLYN
                     EAGSTLTNSGNAIAGGENAIVNITRTSDSLSQVNRGKITATNGYSAIKTASTASNSNG
                     KWIWNTETGVINGINPDAPLIDLGRGYNFANAGTINVQGDGSVAISGGTTSYTVQLVN
                     SGTINVGTEQGKADGSNGEGLIGIKGNGSATTINNTKDGVINVYADNSWAFGGSTKAI
                     VNNGIINLLCNIGCEIYAPNTTGTRNSQDGTADIIVPVASATPGQGNVPAAPVNAVSQ
                     QKLTNYTIGTNSDGSSGTLKANNLVISDNVKVNTGFSAGTADTTVVIDDVFKGENISG
                     AENITSSSVVWNAKGSTDASGNVDVTMSKNAYTDVATDASVNDVAKALDAGYTNNELY
                     TSLNVGTTAELNSALKQVSGSQATTVFREARVLSNRFSMLADAAPKVGNGLAFNVVAK
                     GDPRAELGNNTEYDMLALRKTIDLSESQTMSLEYGIARLDGDGAQKAGDNGVTGGYSQ
                     FFGLKHQMSFDNGMNWNNALRYDVHNLDSSRSIAFSNTNKTADTDVKQQYLEFRSEGA
                     KTFEPSEGLKVTPYAGVKLRHTLEGGYQERNAGDFNLNMNSGSETAVDSIVGLKLDYA
                     GKDGWSASATLEGGPNLSYAKSQRTASLAGAGSQHFNIDDGQKGGGINSLASVGVKYS
                     SKESSLNLDAYNWKEEGISDKGVMLNFKKTF"
BASE COUNT         2921 a         3485 c         2993 g         3549 t
ORIGIN      
        1 gcattttggt tatctataag ttcagcaata tccataacct gcgataaatg ttccactccc
       61 gtaaactcga acctttttcg ttcccttccg gctgtaaatt catcaacata aagttctgcc
      121 gtcaatataa aattatactc ggtgtggtga cattccattc tggtttctac taaaacacca
      181 tcatgccaga taatattgtc taaatttttc atactattaa tccaatccat tactttctga
      241 taaaatttcc tctggaagtt tttcatagaa agagattaaa gcctgaccga cgcaatcaag
      301 ctctattatt cgctcataat acgatctcat taaaatatca tgcttactca ttgcaatacg
      361 tcctgtttac catgggcaga gtatgaatgt aacttatgta aaatagtacc gcccttgcta
      421 tttttcagaa ttcataaaga aaaaacccgg cgctaaatgc ttaccgggtt ttagatttat
      481 caggcaaata gcagtgttaa ccgctaatct gctccatcgc ctgcaaaata cgcttatctg
      541 aaatcggata aggcgtacca agttgttgag cgaagtaact aacgcgcaac tcttctatca
      601 tccaacggat ctctttcacg tcttcatcct cacgacgtgc gggcggcagt ttgttgatcc
      661 attgctgcca cgcctgctgg acattttcga ctttcagcat ctgagcacgg tcgcgatgtg
      721 ggtcaaccgc cagtttttcc agccgttttt caatcgcctg caaataacgc agcgtgtcgc
      781 ccaaccgttt gaagccgtta ctagtgacaa aaccgcgata taccaacccg cccatctgcg
      841 ctttaatgtc agaaagcccc agcgccatgg tcatatccac ccgccctttc agacgtttat
      901 tgatattgaa caccgcagta aggatttgct cgacctgctt cgcaatatcc accaccgtgt
      961 cgttcagttc agcgcggact ttttcatgca gcgcagcaaa accttcttcc gtccagaccg
     1021 ggccaccatt ggcgtcgatc agttgatcca caccgcagga gatacagtcg tcgatcagct
     1081 ccagcacttt gccatacggg ttaaagtaca gccccagctt ggctttgttt ggcaacttct
     1141 catgcaaata tttgattggc gagggaatat tcagcagcag tagacggcga agaccgttcc
     1201 acattgcctg cttttgttcc agcggattat caaacagttt gatcgccacg ctgtcgcgtt
     1261 catccactag cgctggccac gccttcactt tatagttgcc acgcttctgt tcgtagcttt
     1321 ccggcagctg accaaaactc cagatatgta agccgctctg ctcgataccg tcatccgcca
     1381 ccgcagacag cgtttcctgc actttgcctt tcagcgcatc tttcagatct tgtagagagc
     1441 gcccttcttt tagcttcttg tttttgtcat ccaccacacg gaaggtgatt ttcaggtgat
     1501 cgggcacctg atcccagtgc cagtcttcgc ggtcaacggt gacgccagtc atccgccgta
     1561 gctctcgttc aaggctgtcg agcaacggca attccagcgg tgtgacgcgg cctaaaaaag
     1621 cttcggcgta gtttggcgcg ggcacaaaat tacgccgtac cggtttcggc aacgatttaa
     1681 tcagagcaat caccagttcg cggcgcagac cggggatctg ccactcaaat ccgctctcct
     1741 ccacctggtt aagtaacggc agcgggatat gtaccgtcac gccgtccgca tccgcccctg
     1801 gctcaaactg ataactcaaa cgcagcttga gattgccctg atgccagaag ttcgggtaat
     1861 ccagcttgct gatcttttcc gccccttctt tgatcaacat gctcttttca aagttgagca
     1921 aatcaggcgt ttcgcggctg acttttttcc accagctgtc gaagtgacgt gcggagatca
     1981 catcgtggct gatgcgctgg tcgtaaaact caaacagcgt ttcgtcatcc accagaatat
     2041 cgcggcgacg tgttttgtgt tccagttctt ccacttcggc ccgtagtttc aggttttcac
     2101 ggaagaatgc gtgacgcgtc tgccagtcac cttccaccag cgcgtggcga ataaagagtt
     2161 cacgacataa cgccggatcg atctggctgt agttgacctt gcgcgcggca acaatcggca
     2221 agccataaac agtgactttt tccgttgcca tcaccgcgcc ctgcgcccgt tcccagtgcg
     2281 gttcgctgta ggtgcgttta atcaaatgct gagcaactgg ctccacccat tctggatcga
     2341 tgcgtgccgc aatgcgcccc cacaggcggc tggtttctac cagttccgcc accattaccc
     2401 atttcggcgg ctttttaaat aagccggaac cggggaaaat ggagaaacgc gcgttacgtg
     2461 cgccggtata ttcttgttta tcggcatctt tcatgccgat atgggaaagc aaaccggtca
     2521 gtaaggcgat gtgaatttcg cgatactccg ccggttcgct gttaaccgga atgccaagtt
     2581 cttttaccac ctggcgcaac tgggtgtaga tatcctgcca ttcgcgcacg cgcagatagt
     2641 tgagataatc ggtacgacac aggcgacgga aagcgttgga agaaagcgcc ttttgctgct
     2701 cgccaagata attccacaga tttacaaacg cgaggaagtc ggattctttg tcgtggaagc
     2761 gacgatgttt ttcgtccgat gcctgctgtt tgtccatcgg tcgttcgcgc ggatcctgga
     2821 tggagagcgc ggacgtgata atcatcgcct cacgcacgca gccatgtttt tgcgcttcca
     2881 gcaccatacg cgccagacga ggatcgacag gcaattgcga aagctggcga ccgagcggcg
     2941 tcagtttata ggcgctggcc tgttcatcag tggtgatcgc gcccagctct tcgagcagac
     3001 gcacgccatc ctggatatta cgtttatccg gtgcttcgac aaatgggaac gcggcgatat
     3061 cgcccaaccc cagcgcggtc atctgtaaaa taaccgaggc caggttggta cgcagaatct
     3121 ccggatcggt aaactccggg cgcgagagga aatcgtcttc ggaatagaga cgaatacaga
     3181 tcccttcgga cacacgacca cagcggcctt tacgctggtt ggcagacgcc tgcgaaatgg
     3241 gctcaatcgg caaacgctgc actttggtgc gatagctgta gcggctgata cgcgccgtac
     3301 ctgggtcgat aacgtattta atccccggta cggtcagcga cgtttccgcg acgttggtcg
     3361 ccagcacaat gcgccgtcca ctgtgcgact ggaacacgcg gttctgttcg ctgttcgaaa
     3421 gccgcgcata aagcggcaag atttcggtat ggcgtaagtt cagcttgttc agcgcatcgg
     3481 cggtatcgcg aatttcccgc tcaccgctca taaagatcag aatgtcgccg ggactttcct
     3541 ggctcagttc gtctacggcg tcaaaaatcg cctgcaactg atcgcgctcg gtgtcatcgg
     3601 cttcttcaac aatcgggcga tagcgcactt ccaccggata ggtccgaccg gagacttcga
     3661 taatcggcgc attattaaag tggcgcgaaa agcgttccgg gtcgatagtc gcggaagtga
     3721 taatgatttt taggtcagga cgccgcggta gcaactcttt caaatagccg agcagaaaat
     3781 cgatattcag gctgcgttcg tgcgcttcgt caataatgat agtgtcgtac tgcatcagca
     3841 ggcggtcttg ctggatctcc gccagcagga taccgtcggt catcagcttg accatcgtgt
     3901 tatcacttac gtgatcgctg aaacgcactt tgtaaccgat gcaaccgccc ggctccgttt
     3961 tcagctcttc cgcaatacgg ttcgccaccg ttcgtgccgc caggcgacgc ggctgggtat
     4021 ggccgatcag ccctttaatc ccgcgcccca gttccataca gattttcggt agctgagtcg
     4081 tcttaccgga accggtttcc ccggcgacga tcaccacctg gtgatcacga atcgcttcga
     4141 gaatgtcctt ctttttctga ctaactggca aattgtcggg ataagtgatt tccggtcgtg
     4201 ccgcttcacg cagcaggact ttccctgccg cctggtcaat ctctttcgcc atctcctgga
     4261 aaatggcctg ttgtgcatca ggatttttaa ccttcttcac accgtgcaga cggcgtaaaa
     4321 aacgctgtct gtcgcgcagc atcagcgaat ccagccgctg ctgcaaggca gtaaaggtca
     4381 atttttgttg ttctgtcata acgttagtgg gcagtagtct gcctgattaa ttttctttta
     4441 aatgatagat atagagtacc acatcgcggc tttctatgcg ttgttcaata aattcgaaca
     4501 taggcttcga tttattgcgc tatctctgtg gcaggattgt gaataaagtg tcagcaagca
     4561 acggggcatc gcccattcaa acatctataa ggaaacacca tgagcaaggt attagttctt
     4621 aaatccagca tcctggcagg gtactctcag tctaatcagt tgtccgatta ttttgttgaa
     4681 caatggcgcg aaaagcactc cgctgatgaa atcaccgttc gcgacctggc tgcaaatccg
     4741 attccggtgc tggatggcga actggttggt gcgctgcgtc cgagcgatgc accgctgact
     4801 ccgcgtcagc aggaagctct ggcactttcc gatgagctga ttgccgagct gaaagcccac
     4861 gacgttatcg ttattgcggc accgatgtat aacttcaaca tctcgactca gttgaaaaac
     4921 tattttgacc tggttgcacg cgcaggcgtt actttccgct ataccgagaa tggtccggaa
     4981 ggtctggtaa cgggtaaaaa agccatcgtt attaccagcc gcggcgggat ccacaaagat
     5041 ggaccaacgg acctggtgac gccgtatctg tccacgttcc tcggctttat cggcattact
     5101 gatgtgaaat ttgtcttcgc cgaagggatc gcatacggtc ctgaaatggc agcgaaagcg
     5161 cagtctgacg cgaaagcagc tatcgacagt attgttgctg aataaccgga cacgatcttg
     5221 atgtggactg tagatattca gtccacatct caatccgctt acctgttttc tatcgctttg
     5281 tgctcttcct taagcacaat ctgcgagcgc ctgactcgtt attctcgcga aataccatcc
     5341 aacgtagaca acacttcctc agaaagatgc aatttttcag cagccatatt ctcccgtaaa
     5401 tgcgcaacag aagatgtccc tgggatcagc aaaatattcg gtgaacgttg taacagccac
     5461 gccagcgcca cctgcattgg tgttgcgccc aggctcgctg ctacatccga aagtgtggag
     5521 gattgcagcg gcgtaaagcc cccaagcggg aagaacggca cgtaagcaat gccaacgcgg
     5581 gccaaatcat caatcattgc gtcatcagca cgatgagcaa tgttgtattc gttctgcaca
     5641 cagacgattt cggtaatctt acgcgcctct gcgacctgcg tcggtgtgac gttgctcagg
     5701 ccaatatgtt ttaccaggcc ttgttgttgc atctctgcca gcacggtcag gctggcttca
     5761 atcgatcctt ccgccgggcc atgaccatcc cccatcatga cgcgcaggtt aaccacatcc
     5821 agtacgtcca ggccgagatt acgtagatta tcgtgtaccg ctttttgcag ctctgccgga
     5881 gaaaatgcgg gcaaccagga agcatcctca ccacgtcgtg caccaatttt agtgacaatt
     5941 gtcaggtcgt cagagtaagg atgtagcgct tcgcggataa tctgattggt gacgtgcgga
     6001 ccataaaagt cgctggtatc aatatgattg acgcccaatg ccagcgcctc gcgcagcacg
     6061 gttatagcaa cgtggcgatc tcttgggggg ccaaaaaccc cagggcctgc cagttgcatc
     6121 gcgccataac caagacggtt aacggatttt gtaccgagag taaatgtgtt gctgctcatc
     6181 gcaccctgcc ctgtaataaa acggattaag aaaaatcagt atagaaacga aaagagataa
     6241 ataatgcgaa ggcgtaaaca agattactgg atgagttgct ctattgtcct tgaagacacc
     6301 aaaacaaaac gcctaaagca tcgcatccgg caataaaaaa ccgcaacctg tatcagcatt
     6361 gcggtttgtg tatcacgtgc taaaaattag aacgttttct tgaagttcag catcacgcct
     6421 ttgtcgctga tgccttcctc tttccagttg tacgcatcca gattcagcga actttctttg
     6481 ctgctgtact tcacgccgac acttgccagg ctgttgatgc cgccgccctt ctgaccgtca
     6541 tcgatgttaa agtgctgact gcctgcacct gccaggcttg ctgtgcgctg gctcttcgcg
     6601 tagctcaagt tcggcccgcc ttccagcgta gcgctagcgc tccagccgtc tttacctgca
     6661 tagtccagtt tcagcccgac gatgctgtcc accgccgttt cgctaccact gttcatattc
     6721 aggttaaagt ctccggcatt gcgctcctga tagccacctt ccagtgtgtg acgcagtttc
     6781 acacccgcat atggtgtgac cttcagccct tcgctcggtt cgaaagtctt cgctccttcg
     6841 ctgcggaact ccaggtactg ctgtttcacg tcggtatcag cagttttgtt cgtgttgcta
     6901 aatgcaatcg aacggctgct gtcaaggttg tgaacgtcat aacgcaatgc gttgttccag
     6961 ttcatgccat tgtcgaagga catctgatgt ttcaggccaa agaactggct gtaaccgcct
     7021 gtaacaccat tatcgccagc tttctgcgca ccgtcgccat cgagacgagc gataccgtac
     7081 tccagactca tggtctgact ttcactcagg tcaatggttt tacgcagtgc cagcatgtcg
     7141 tactcggtat tgttccccag ctccgcgcgc ggatcgcctt tcgccaccac gttgaacgcc
     7201 agaccgttac ccactttcgg tgcggcatct gccagcatgc taaagcggtt gctgagcaca
     7261 cgcgcttcgc ggaataccgt ggtcgcctgg ctaccgctga cctgtttcag ggcgctgttc
     7321 agttcggcag tcgtgccgac gttcaggctg gtatacagct cgttgttggt gtaacccgca
     7381 tccagtgctt tcgcgacgtc attcaccgaa gcatcggtcg ccacatcggt ataagcgttt
     7441 ttgctcatag tgacgtcaac gttgccgctg gcatcggtac tgcctttagc gttccagaca
     7501 actgaagagg aagtgatatt ctccgcgccg ctgatatttt cacctttgaa aacatcatca
     7561 atcaccacgg tagtatcagc agttcctgca ctaaagccag tatttacctt gacgttgtca
     7621 ctgataacaa ggttattggc tttcaaggtg ccggaacttc cgtcgctatt agtaccaata
     7681 gtgtaattgg taagtttttg ctgacttaca gcattgactg gtgccgcagg aacattacct
     7741 tgcccaggcg tcgcagaagc gaccggtacg attatatccg cagttccatc ctgactgttt
     7801 ctcgtccctg tggtatttgg cgcataaatc tcgcaaccaa tattacacaa caggttgatt
     7861 atcccattat tcacaatcgc tttagtggaa ccaccaaacg cccatgaatt atctgcataa
     7921 acgttaatga caccgtcttt agtattatta atcgttgttg cagaaccatt tcctttgatg
     7981 ccaatcagac cttcaccgtt gcttccatct gctttgccct gttcagtacc cacgttaatt
     8041 gtgccgctgt tcactaactg aacagtatag ctggtggtgc ctccgctaat agcaactgaa
     8101 ccatcaccct gaacattaat tgttccggcg ttagcaaaat tatacccgcg acctaaatca
     8161 atcagtggag catctggatt gataccatta attacaccgg tttctgtatt ccaaatccat
     8221 ttgccgttgc tgttagaggc tgtgctggca gttttaatcg cactgtaacc gtttgtggcg
     8281 gtaatttttc cgcggttaac ctgtgaaaga gaatcactgg tgcgtgtgat attaacgatg
     8341 gcattttccc ctcccgcaat agcattgccg ctatttgtaa gcgttgaacc ggcttcatta
     8401 taaagtacga aactgccgtt aattaaggca ctaccatctg tgacagttgt catctccccc
     8461 gtatttacaa cgttttgttt ataactatca ccgctaatta ctccgctaaa gcttccctca
     8521 ttgaagaagg cagacccgcc ttttaacatt aacagttcgg ttgtcatact gcccgaggcg
     8581 cgattgtaaa cactggcatt ggcagtaagc ttgtcggcat ccatgctccc gctgttttcc
     8641 agcccctgga caatattcaa atcaccatta actgtcaggc tggccttatc ctcgttatat
     8701 aaccataggt tggcatcgac atcgccgttc agtatttctg taccataatt agccagcggt
     8761 ttaatggtaa caaagccatc tcggataact gtttttgtaa tgttctctcc actggcactc
     8821 aattcagata acagttccag attctctgca ggagcaccat aatgagagtc ggtgctatca
     8881 atttccgctc cgttgagatt aatctcacca tagttgagga taatgcctgt accatcatta
     8941 taaaacgcct ggcccgtatc aacattaata ttgattacac cagtttggtc gtttattgcc
     9001 gtagcacctt tatacactgc catccctacc agcgtattac tacccaggtt tgcatcgtaa
     9061 tctgcatttt tctcgaggtt aatgattccc tggttaataa ccatattact gttaccgtag
     9121 gcggccatac cggcacctgc gttatataca gtaattacac caccttccag gttggtcgca
     9181 atagcgttgt tacgagcgcc acccccggtg tctgtcccgg ttgccatacc aacgccataa
     9241 tcttttgccg ttgcccccgg aagatcggta tgcaaactgg tggtgtcatt gtcatcaatc
     9301 cataacgtat ccaccgtaat attgcctgca ttatccgccg ttgagttatc gatggctaac
     9361 atgccaatgc taccgcgtcc atacatatca atgacagcat cttcggcatt gagaacagag
     9421 cctttattat acgcctccat ccccatcatg cttgctacgc tgttattaaa cacgcgctga
     9481 tccgttgacc ccgtgagtgc agccatatta acaacgctat gttgctccat tacttttgag
     9541 gtaattaccc ctttgtttac tgcgctgccg ccgttagagg tcaagaaggc cgtgttatct
     9601 ggttccggtg ctggcgtatt agttgaggca taatcataca gagaaagtgt gatattcccg
     9661 ttattgatac ctgtggtatt ttcgccaaag ataccagcaa aactgatatt ccagatatca
     9721 acattaccgt tatttatgac ctcagagcta ctttctgcct ttataatctg tggtgtttgt
     9781 gcatagtaat gatcataatt ttcaatatcc cgcatattga aagtagcttc actgccaatc
     9841 tcaatagctg ctttattttt taactggata aggcttgcat atcctagttg cagatcacca
     9901 ctgatcgttg agtcaccatt cagatatacc ttcgattcac catcaatacc aataagtggc
     9961 gtattaccga agaaagtccg tttgttagca atggctgaac catcggtcag cgcatttatt
    10021 tcaccaacaa atgcaatccc gccattcaga ttgatgttgt taccatcacc ggaaacaata
    10081 agaccagaaa tattttcctg gctaccatca aaattcgaga catcacgtgt ggtagtttca
    10141 ccatccgcaa caagcgttaa tttaccatcc agtgtcacat tgttattatg accactgaca
    10201 tttacgccca cggaagcgtc ataaaaatac tctgccgcat tgtctgcgct ctgatcttta
    10261 tcaaccagaa tattaccagt tatatcaatt gtgttggcat caccggagac gttaatacct
    10321 gtagctttct gaccaacgac attaagatcc tgcattgcca ttgtcaggct attattatcg
    10381 ccagttaatg tcagccctgt tgaaaaatca ccgacctgca tacttcccgc gagggagatt
    10441 ttcccctcgt cgctggtaat agcaaatccc gtcgctgaat tactgacatc cacatcacca
    10501 gtattggcaa gtgtcgcgtt gtcgccattg atcagtacac cgatcgagtc tgcgtccgtg
    10561 acggtcatat tgcctttgtt atcgacggcg gcattatcgc cagaaatatc aatgccgtga
    10621 ccgccgccgc tgacatccag atcaccgtcc tggataactt ttccgttatt gccgttgatt
    10681 tccgtgccgg tggaatcctt gccgtcaacg gtagttttgc cgttgttgtt cgccgtggcg
    10741 tcatcaccgt taatctgcgt gccggtgccg ccgttggtga tggtgctctc gccttcgtta
    10801 ttgacgatgg ccttgtcgcc gtcgatctgg atccccatgg actccggatc ggtgacggtc
    10861 atggtgccct tgttatccac cgtcgcgctg tcaccggtaa tatcaatccc gtgaccgcca
    10921 ccgctgacat ccagatcgcc gtcctgaatc acttttccgt tattaccgtt gatttccgta
    10981 ccggtggaat ctttaccgtc aacggtggtt ttgccgttgt tgtttgccgt ggcgtcatca
    11041 ccgttaatct gtgtgccggt gccgccgttg ctgatggtgc tgtcgccttc gttattgacg
    11101 atggccttgt cgccgtcgat ctgaataccg atggactccg gatcggtgac agtcatggta
    11161 cccttgttgt ccaccgttgc gctgtcaccg gtgatatcaa tgccgtgacc gccgccgctg
    11221 acatccagat cgccgtcctg gataactttt ccgttattac cgttgatttc cgtgccggtg
    11281 gagtctttac cgtcaacggt ggttttgccg ctgttgttcg ccgtggcgtc atctccgtta
    11341 atctgcgtac cggtgccgcc gttgctgatg gaactgtcgc cttcgttatt gacaaccgcc
    11401 ttgtcgccgt cgatctggat accgatggac tccggatcgg taacggtcat ggtgcccttg
    11461 ttatccaccg tcgcgctgtc accggtgata tcaatgccgt ggcccccgcc gctgacatcc
    11521 agatcgccgc cctgaatcac ttttccgtta ttaccgttga tttctgtacc ggtggaatct
    11581 ttaccgtcaa cggtagtttt gccgttgttg ttcgccgtgg cgtcatcacc gttaatctgc
    11641 gtaccggtgc cgccgttggt gatggtgctc tcgccttcgt tattgacgat ggccttgtcg
    11701 ccgtcgatct ggatccccat ggactccgga tcggtgacgg tcatggtgcc cttgttgtcc
    11761 accgttgcgc tgtcgccggt aatatcaatc ccgtgaccgc caccgctgac atccagatcg
    11821 ccatcctgga taaccttccc gttattaccg ttgatttctg taccggtgga atccttgccg
    11881 tcaacggtgg ttttgccgtt gttattcgcc gtggcatcat catcgttaat ctgtgtgccg
    11941 gtgccaccat tgctgatggt gctgtcgcct tcgttgttaa caaccgcctt gtcgccgtcg
    12001 atctgaatac cgatggactc cggatcggtg acggtcatgg tgcccttgtt atccaccgtc
    12061 gcgctgtcgc cggtgatatc aatgccgtga ccgccgccgc tgacatccag atcgccgtcc
    12121 tggataactt ttccgttatt accgttgatt tccgtgccgg tggagtcttt accgtcaacg
    12181 gtggttttgc cattgttgtt tactgtggca ttatcacccg ttataacaag ctcaccgttg
    12241 tgattatatc gccactttgt tgtgccatct gcgctcacac cttcaagggc aacagtatta
    12301 ttcgcttcat caatctgcca aagattaatc gttgccttac gaccactgtt atcttgcaga
    12361 gaaatagtcc cgtcagcatt ctcggtataa gtaaaaactg aatcgcgaat agttaacgtt
    12421 ttttctgttt tatcgagaat aacgtcatta ttaaacgaaa tcggtttggg aggtgtaggc
    12481 gtatcatccg gaacgtccgg tattatctca tctccgccag gagttggggg cactggagtg
    12541 tcgtctgaat catcatcagg gggcgtcggt gggagtggag aattgttgcg atgatgatga
    12601 tcatcatcgt cgtttagttc cactacggct aaggtcgtga ttaacgcagc agcgcctgtg
    12661 gcaagccatg gcatcaggtt ttgttcgact aaaggggtgc attcagaggg taagcgttta
    12721 agctcttccg gactgagttt tcctggatct gccggacaag tcacattagt atttttcttt
    12781 tcacctgtgg tggtttctac ctctgtgata tctgccgccc aaccctgacc actcagagct
    12841 aatgcaatac aggccgacaa taaagttttc ctttgcataa taaatccttc tggcgaatat
    12901 aaagaaagcc ggatgcacta acccgaccaa ttagttattt aaaacatt
//
LOCUS       DACBVI010000124        12938 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.124, whole genome shotgun sequence.
ACCESSION   DACBVI010000124 DACBVI010000000
VERSION     DACBVI010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.124"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            69..293
                     /locus_tag="IU325_17215"
     CDS             69..293
                     /locus_tag="IU325_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HAP5810740.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(361..1083)
                     /gene="sdiA"
                     /locus_tag="IU325_17220"
     CDS             complement(361..1083)
                     /gene="sdiA"
                     /locus_tag="IU325_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HAP5810741.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMETAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(1313..2065)
                     /gene="yecC"
                     /locus_tag="IU325_17225"
     CDS             complement(1313..2065)
                     /gene="yecC"
                     /locus_tag="IU325_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HAP5810742.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPKQPRTRQFLEKFLLQ"
     gene            complement(2062..2730)
                     /gene="tcyL"
                     /locus_tag="IU325_17230"
     CDS             complement(2062..2730)
                     /gene="tcyL"
                     /locus_tag="IU325_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HAP5810743.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(2745..3731)
                     /gene="dcyD"
                     /locus_tag="IU325_17235"
     CDS             complement(2745..3731)
                     /gene="dcyD"
                     /locus_tag="IU325_17235"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HAP5810744.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(3836..4636)
                     /gene="tcyJ"
                     /locus_tag="IU325_17240"
     CDS             complement(3836..4636)
                     /gene="tcyJ"
                     /locus_tag="IU325_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP5810745.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(4724..5275)
                     /gene="fliZ"
                     /locus_tag="IU325_17245"
     CDS             complement(4724..5275)
                     /gene="fliZ"
                     /locus_tag="IU325_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HAP5810746.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTRLVQKSSSLPSSDIY"
     gene            complement(5321..6040)
                     /locus_tag="IU325_17250"
     CDS             complement(5321..6040)
                     /locus_tag="IU325_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HAP5810747.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(6359..8371)
                     /locus_tag="IU325_17255"
     CDS             complement(6359..8371)
                     /locus_tag="IU325_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HAP5810748.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQATTG
                     TNSTSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLSKDGSMKIQVGANDGETITIDL
                     KKIDSDTLNLAGFNVNGKGSVANTAATSDDLKLAGFTKGTTDTNGVTAYTNTISNDKA
                     KASDLLANITDGSVITGGGANAFGVAAKNGYTYDAASKSYSFAADGADSAKTLSIINP
                     NTGDSSQATVTIGGKEQKVNISQDGKITAADDNATLYLDKQGNLTKTNAGNDTAATWD
                     GLISNSDSTGAVPVGVATTITITSGTASGMSVQSAGAGIQTSTNSQILAGGAFAAKVS
                     IEGGAATDILVASNGNITAADGSALYLDATTGGFTTTAGGNTAASLDNLIANSKDATL
                     TVTSGTGQNTVYSTTGSGAQFTSLAKVDTVNVTNAHVSAEGMANLTKSNFTIDMGGTG
                     TVTYTVSNGDVKAAANADVYVEDGALSANATKDVTYFEQKNGAITNSTGGTIYETADG
                     KLTTEATTASSSTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEA
                     QSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            8637..10043
                     /gene="fliD"
                     /locus_tag="IU325_17260"
     CDS             8637..10043
                     /gene="fliD"
                     /locus_tag="IU325_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HAP5810749.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            10068..10478
                     /gene="fliS"
                     /locus_tag="IU325_17265"
     CDS             10068..10478
                     /gene="fliS"
                     /locus_tag="IU325_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HAP5810750.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVETLMRNIADAWKESLLSPSLIQDPV"
     gene            10478..10843
                     /gene="fliT"
                     /locus_tag="IU325_17270"
     CDS             10478..10843
                     /gene="fliT"
                     /locus_tag="IU325_17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HAP5810751.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDKLIASEMAYVNAV
                     QEIAHLTEEIDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            10921..12408
                     /gene="amyA"
                     /locus_tag="IU325_17275"
     CDS             10921..12408
                     /gene="amyA"
                     /locus_tag="IU325_17275"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAP5810752.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(12442..12855)
                     /locus_tag="IU325_17280"
     CDS             complement(12442..12855)
                     /locus_tag="IU325_17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP5810753.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
BASE COUNT         3245 a         3319 c         3131 g         3243 t
ORIGIN      
        1 tatggtatac tccgccgcct taacattttt caccgcaaat tttcattgca acatgacgag
       61 gaaaataaat gagtacgcct gatttttcta ctgccgagaa taatcaagaa ctggcaaatg
      121 aagtctcctg cctgaaagcg atgctgacgc tgatgctgca ggcgatggga caagctgacg
      181 cgggccgcgt gatgttaaag atggaaaaac agcttgcgct gatcgaagac gaaacccagg
      241 ctgcagtatt ttccaaaacg gttaagcaaa ttaaacaagc ctaccgtcag taatataaaa
      301 ccggctgata gcgtgccttt cagccggttt ttgcatctgg cacgcaggac agaaaagaga
      361 tcaaattaag ccagtagcgg ccgcgtaaca ggcaacctgg gtcttatttg gtgcattaat
      421 ttttttctgc atgtttttct ggtggaaatt gaccgtattc tcagagattg acaaaatcat
      481 cgctatctct gctgatgtct tcccttccgc cgtccacctc agaatttctt tttcgcgctt
      541 gctgaaattc atctctggcg tcatcactat ttcatcattt aaacgcatca gagccatcag
      601 actttcgcgc accagtaact gcatttttaa ttgcagttca tcactaagaa tgggtatttc
      661 gcgcgcgctg caacgggaaa aggacaaaaa gcccagcgcc cggtttggca gcattaaata
      721 ctgagtgaca ccgcggcgta aaccatgtgc gcgcgcggct tcccataatg gctgtgcttc
      781 gctgaataag tcatcattcc acattaaatg gccctgacta aagttttcag ggttcagcac
      841 cggatcaatt gcgagaaagt tttttgcctg ataataacta acccacgcct cagggtaatt
      901 ggtgtagaaa gccactttag gtcgcgtgaa tggtaccggg tggcggacac ataacgaata
      961 gtaatcgtac tccagctgct gagcctgaag ctcaatttca tggtagacct cttctgcggt
     1021 ctccatcctc tgaaaacgca acagcatcgt gcgacgccag ctgaaaaaat ccgtatcctg
     1081 catagtaaac cgcaacgccc ctgagagtga gtatcattta taatgataat tcaaactatc
     1141 acataaataa gatttatata taatttatat tattcagaca atgaattact tttgcaagcc
     1201 atcgcattct cttatgttat taatgagtta tgctgatttg ttaagcagtt ttatcacgct
     1261 tgaaatggcg tcccgccccg acaggtgaat cgtcggggct gattttttct tattattgca
     1321 gcagaaactt ctcgaggaac tggcgggtgc gaggctgctt gggatcggca aataacgctt
     1381 ttgcggcccc ctgctcgact atccgcccct ggtccataaa gatcgcccgg tccgcaacat
     1441 cccgggcaaa gctcatttcg tgtgtcacaa tcaccatcgt gcgcttttcc tgcgccagct
     1501 gacggatggt gttcaggact tcgcccacca gctctggatc cagcgctgac gttggctcgt
     1561 caaacaaaat cacctcagga cgcattgcca gcgcacgcgc aatcgcaaca cgctgctgtt
     1621 gaccgccaga caaacgacgt ggatagctgg tttctttacc ggccagccca actttcgcca
     1681 acagctcgcg agcgcgcgcc gtggcctctt ctttcggttc gcctttgacg atcaccggcc
     1741 cttcaataat gttctccagc accgtacgat gcggaaacaa attaaagttc tggaagacaa
     1801 acccgacgtg ctgacgcaac tggcgaatca gcgatttttg ctgacttaat gaacgtgcag
     1861 tatcaatagt gatatcgcca acggtgatcg ttcccgcttc cggttgttcc agcagattta
     1921 tgctgcgtag caacgtggtt ttgccggaac cactcggacc gataattgcc accacttcgc
     1981 caggctttac ctcgaggtcg ataccgtgca gcaccgtctg accgtgaaat tttttcacca
     2041 ggttcttaac ttcaatggca ctcattttgg ttctctctcc tggcgattaa gttgattctc
     2101 aaaatggttc tgcaacgtcg ataatactgt cgccatgatc cagtagatga gcgaagccgc
     2161 caggtacatg gtgaacacct ccagcgtgcg cgaggtaatc aactgcgctt gacggaacag
     2221 ctccggcacc tggattgtcg cggccagcga cgtatctttt accaggctga taaagctgtt
     2281 cgacagcggc ggcaacgcca cccgcgccgc ctgtggcaaa atcgcgcgac gcatagtctg
     2341 ccatggcgtc ataccaatac tggcagctgc ttcccactga cctttatcaa tagaagaaat
     2401 ggcggcgcgc agcgtttcgg cagcataggc ggcagtgttg agcgacagac caatcattgc
     2461 tgacggaatg ggatctaatt cgataccaaa ctgcggcagg ccgtaataga tcataaacag
     2521 ttgtgcgatg agcggcgtac cacgaaaaat agagatataa aaacgcgcca gccagcgcac
     2581 gggccagatt ggcgacaggc gcatcagcgc gagaataaac ccgagtaata agccaaaaaa
     2641 catgccgccg atactgagtt gcagcgtata cccagcccct ttcagcagga acggcaagga
     2701 atcaataacc agttgtatac tttcttgcat gagcgttccc ggagctaaac gtggggatga
     2761 taggcgaaca gcgcaggcgc accgccggta tgaataaaca gaatcggccc ttcatctttg
     2821 aagcgtttct gactgatacc gtcaatcaga cccgccatcg cttttccggt atacacagga
     2881 tcaagcagaa tgccttccag ccgcgccagc aatttcactg cttccatgcc ttcgtcgttc
     2941 ggcacgccgt agccaggtgc aaaatagtca tcccagagta aaatttccgc tgatgcggtc
     3001 agctccagtt ctttcgcaat cgcctgttgt aggttaacca ctttcggcaa ttgatcggca
     3061 acagaacgcg acacggtcac gccaatcagt tcgctttcag gcatcaggtg ttccagccca
     3121 acagccagtc cggcgtgagt tccggcactg cccgatgcga ccaccaccga cgaaatatta
     3181 accgcccctt cacactgttg cgcgatttcc agcgcactct ccacataacc tagcgcgccc
     3241 agagcattag aaccgccaac cggaatgaca tacgggcgaa agccttgtgc ttcgactcgc
     3301 gtcgccagct cttccagttg ggcattggga tcggtcagtg cgtcgcacat ttcaatctgg
     3361 gtattgaaca gatccagcaa caaacgattg ccgttggtta aatagttttc tgcggttgtg
     3421 ccaataggat tttccagcag cgccacgcag tgcagaccga gtttcgccgc gactgcggca
     3481 gtctgccgca catggttaga ctggatcgcc ccggcagtaa tcagcgtatc ggcaccttcg
     3541 cgcagggcat ctgccgcgag aaattccagc ttacgtaatt tattgccgcc cattgccatg
     3601 ggggtgacgt catcccgttt gatgaaaatt tcccgtccta gataatcaga aaagcgcggc
     3661 agatattcga gcggcgttgg cgcgccgata aactccaacc gtggaaaacg ggttaaatta
     3721 tgcagtggca taacagcctc cgatgtgtgt tgttgtgatt ttcttattat gcacgctgaa
     3781 aacgcgtaaa taaaaaaggc gctagtgaaa gcgccctttt ttgtcattat gctgattatt
     3841 tggtcacatc agcaccaaac catttttcgg aaagggcttg cagagtgcca tctttttgca
     3901 tttccgcaat tgcatcattc actgctttca gcaggtcttc atttccttta cgcagcgcca
     3961 cgccagactc ctgacgggag aatgcttcac cggttactgc cagcgtatcg ttggttttct
     4021 tcaccagatc cagcgccgcc agacggtcaa cgaggatcgc atcgatacgg cctacgcgca
     4081 gatcctgata tttggtcggg tcatcatcat aggtacgcac atcgacgccc tgaacattct
     4141 gacgcagcca ctcttcatag ttggtgccca gaccgacacc cacttttttg cctttcagat
     4201 cagcagctgt tttaatggtg ccttcgttac cttttttcac cagcgcctga ataccggaaa
     4261 tggtgtaagg ggtagagaaa tcgtattttt tcttgcgctc gtcagaaatg gtgacctgat
     4321 taatcaccac atcaatgcgt ttagaatcca gcgacgccag cataccgtcc catttggtcg
     4381 gttttaggga cgcctcaacg ccaagatgtt ttgccagctg ttgggcaaat tccacttcaa
     4441 aaccggttaa tttgccgtca tccccctgaa aactgaacgg cggataagtt ccttccagcc
     4501 ctaccagcag cgtgccgcgc tctttgactt tattaagcag accttcatct gcaaaacttt
     4561 taacgctcat gcccgcaacc agcgccacgg ccatcacacc catcaatgcc tgacgtccca
     4621 gatgtgctaa tttcatattc accccgaatg ttgttatgtc tgtttgcagt gtagagccat
     4681 tgtttgtaaa cacaaaaaca actccgctac atcttattct tatttaatat atatcagaag
     4741 aaggcaggct ggaggatttc tgcacaagtc tggtacaggt ttggcgctga tagtgttgat
     4801 acttccgtaa cgcgatgcgg taattgttgg tgctggtggt gggcagccac ggggcgagga
     4861 tttcatcaag gaaaccgtcc tgcagctgat cgagggaaat attttgctcg tgtagatgat
     4921 tccccaaacg gcgcaaacga acgacatatt cacgcaccgt acctgggctc atttcagttt
     4981 gctcaaaaag aaattgctta aagccgataa tatcgaaaaa atcactctgc gttttgcaat
     5041 gtaaatcacc gcaaaatcgg cacaatgccg cccatgattt ttgttccgtt tgccagccat
     5101 tttcatcaag cagcgtgtcc aggcgggaaa tctcgatttt attcactatt ttgccgtcgc
     5161 gaactaaggt aatgcgatcg agtaatttac ggcaatgcgc gcaatgcgtc tggctgtgtt
     5221 taaagtcttt aagatagcgg cttaatggcc gtcttttcag gtgctgcacc atcattaaga
     5281 actcctggta gtaaaagtta aagtgcggca tttactgacg ttataactta cccagtttag
     5341 tgcgtaaccg tttaatagcc tggctgtgta actgactgac ccgcgattcc ccgacctcca
     5401 gcactgcacc aatctctttg agattcagct cttcctggta atagagggtt aataccagtt
     5461 tttcgcgctc cggcaacgtt tcgatggctt ccatcacccg ctggcgcaga ttactgtcca
     5521 gcagttgttg tagcgggttt tctcgctgat gatcatcagt aaccagttcg atgctatcgc
     5581 cgtgctcttc gcgccactca tcgtaggaga agagctggct gttattggtg tcgagcaaca
     5641 tttggcgata atcggcaata tcgatcccta aacgttccgc tacctcagtt tccgtggcgt
     5701 tgcggccaag ttcctgctcc agttgcccta ttgcctgtgc cacttcacgc gcgttgcgac
     5761 gcacgctgcg cggcacccag tcacggctgc gaagttcatc cagcatagcg ccacggatac
     5821 gctgcactgc gtaagttgta aatgccgttc cttgtagggc gtcatagcgt tcgacggcat
     5881 taagtaaccc aatgccgccc gcctgtagca gatcgtcaag ttccacgctc gcgggcagtc
     5941 gaacctgcag gcgcaatgct tcgtgacgca ccagcgggac ataacgctgc cacagcgagt
     6001 gtttatccat tacaccttca gcggtataga gtgaattcac gataaacagc cctgcgttat
     6061 atgagttatc ggcatgatta tccgtttctg cagggttttt aatcggacga ttagtgggtg
     6121 aaatgagggg ttatttgggg gttacaggta aattccaggc agaaaaaacc ccgccggtgg
     6181 cggggaagca cgttgctgac aattgcgctt tatgttgccg gatgcggcgt aaacgcctta
     6241 tccggcctac aaaaatgtgc aaattcaata aattgcaatt caacttgtag gcctgataag
     6301 cgcagtgcat caggcaattt ggcgttgccg tcagtctcag ttaatcaggt taggacgatt
     6361 aaccctgcag cagagacaga acctgctgcg gtacctggtt ggcttttgcc agcacggagt
     6421 taccggcctg ctggataatc tgcgctttcg acatgttgga cacttcggtc gcatagtcgg
     6481 cgtcctgaat acgggactgc gcttcggaca ggttggtagt ggtgttgttc aggttggtga
     6541 ctgcggaatc cagacggttc tgcaccgcac ccagggaaga acggaatttg tcgatggagc
     6601 tgatggcttc gtccagagct ttcaggggat cggcggtgga actggatgca gtagtagctt
     6661 ctgttgttaa cttaccatca gctgtttcat agatggtacc accggtgctg ttggtaatag
     6721 ccccattttt ttgttcaaag taggttacat cttttgtagc attggctgaa agtgcaccat
     6781 cttcgacata aacatcagca tttgcagcag ctttcacatc cccattggaa actgtgtaag
     6841 ttactgtacc tgtaccgccc atatcaatgg taaaattgct ttttgtcaga tttgccatac
     6901 cttcggcact gacatgtgcg ttggtgacat tgacagtgtc tacttttgct aaactggtga
     6961 actgagcgcc acttcctgtt gtgctataaa cagtgttctg gccggtacct gaagttacgg
     7021 ttaaggtagc atccttactg ttagcaatta aattatctaa cgaagcagct gtatttcctc
     7081 cagccgttgt agtgaatcca ccagtagtcg catcaagata aagtgcacta ccatcagccg
     7141 ctgttatgtt tccattactt gctaccaaaa tgtctgtagc agcgcctccc tcaatactta
     7201 ccttagccgc aaatgcacca cctgcaagaa tctgagaatt tgttgaggtc tgaattcctg
     7261 ctcctgcgga ctgaacagac attccggaag ctgtaccaga agtaattgta attgtagttg
     7321 caaccccaac tggaaccgca ccggtagaat cgctgttgga aattaaacca tcccaagtcg
     7381 ctgcggtatc gttacctgca ttcgtttttg tcaagtttcc ctgtttatct aaatacagcg
     7441 tcgcattatc atctgccgca gtaatttttc catcctggga aatattaact ttctgctctt
     7501 taccaccaat agtcactgtc gcctgcgacg aatcaccggt gtttggatta atgatgctta
     7561 acgtcttcgc tgaatcggca ccatctgcag caaaactata agatttactt gctgcatcat
     7621 aggtgtaacc attctttgca gccacgccaa aagcgtttgc ccctccccca gtgatcactg
     7681 atccatcggt gatattagct aacagatcgg aagctttggc tttgtcatta ctaattgtgt
     7741 ttgtatacgc ggtcacgcca ttggtatctg tggtgccctt agtgaaacca gccagtttta
     7801 aatcgtcgct tgtcgcagct gtattcgcta cagaaccttt accgttaacg ttaaaaccag
     7861 ccagattcag cgtatcagag tcaattttct tcagatcaat agtaatcgtt tcgccatcgt
     7921 tcgcgccgac ctgaattttc atcgagccat cttttgacag tacgttcacg ccgttgaact
     7981 gggtttggcc ggatacgcgg tcaatttcat caagacggga tttgatttcg tcctgaatgg
     8041 agtccaggtc agaggtggag ttagttccgg tcgtcgcctg aaccgtcagt tcacgaatac
     8101 gctgtaagtt gttgttgatt tcggacagcg cgccttcggt ggtctgcgca acagaaatac
     8161 cgtcgttggc gttacgtgca gcctgagtca ggcctttaat gttagaagta aaacggttag
     8221 caatcgcctg acctgcggcg tcatccttcg cgctgttaat acgcaagcca gaagacagac
     8281 gctcgataga actcgacagc gcagactggt tcttgttgat attattttga gtgatcagcg
     8341 agaggctgtt ggtattaatg acttgtgcca tgattcgtta tcctatattg caagtcgttg
     8401 attacgtgtt gggtttccac ccgtcggctc aatcgccgtc aaccctgtta tcgtctgtcg
     8461 taaaacaacc tttagaattt ttttcacaaa cagccatttt ttgttagtcg ccgaaatact
     8521 cttttctctg ccccttattc ccgctattaa aaaaaacaat taaacgtaaa ctttgcgcaa
     8581 ttcaggccga taaccccagt attcgtttta cgtgtcgaaa gataaaagga aatcgcatgg
     8641 caagtatttc atcgctggga gtcgggtcag gcctggattt aagttcaatc cttgatagcc
     8701 tcaccgccgc gcaaaaagcg acgctaaccc ctatttcaaa tcagcaatcg tcgtttaccg
     8761 ctaaacttag cgcctacggt acgctgaaaa gcgcgttgac gactttccag accgccaata
     8821 ctgcattgtc taaagccgat cttttttccg ccaccagcac taccagcagc accaccgcgt
     8881 tcagtgccac caccgcgggt aacgccatcg ccgggaaata caccatcagc gtcacccatc
     8941 tggcgcaggc gcaaaccctg accacgcgca ccaccagaga tgatacgaaa acggcgatcg
     9001 ccaccagcga cagtaaactc accattcaac aaggcggcga caaagatccg attaccattg
     9061 atatcagcgc ggctaactcg tcgttaagcg ggatccgtga tgccatcaac aacgcaaaag
     9121 cgggcgtaag tgcgagcatc attaatgtgg gtaacggtga atatcgtctg tcagtcacat
     9181 caaatgacac cggccttgat aatgcgatga cactctcggt cagcggtgat gatgcgctac
     9241 aaagttttat gggctatgac gccagtgcca gcagcaacgg tatggaagtc tcggtcgccg
     9301 cccagaatgc gcagctgacg gtcaacaacg tcgccattga gaacagcagc aacaccatca
     9361 gcgacgcgct ggaaaacatc accctgaacc tgaacgatgt caccacgggc aaccagacgc
     9421 taaccatcac tcaggacacc tccaaagcgc aaacggcgat taaagattgg gtgaatgcct
     9481 ataactcgct aatagatacc ttcagcagcc tgaccaaata caccgccgta gatgcgggag
     9541 ctgatagcca gagttctagc aatggcgcac tgctcggcga ctccacgctg cggacgattc
     9601 agacgcagtt gaagtcgatg ctgagtaata ccgtcagttc ttccagctat aaaacgttgg
     9661 cgcagattgg tatcacgacc gatcccagcg atggcaaact ggaactggat gccgacaaac
     9721 tcaccgctgc actgaaaaaa gatgccagcg gcgtaggtgc attgattgtt ggcgatggta
     9781 aaaaaaccgg catcacgacc accatcggca gcaacctgac cagttggctt tcgacaacgg
     9841 gcattattaa agccgctacc gatggcgtta gtaagaccct gaataaatta actaaagact
     9901 acaacgccgc cagcgatcgc attgatgcgc aggtcgcgcg ctacaaagaa caatttaccc
     9961 aactagacgt tttaatgacc tcgttaaaca gcacaagcag ctacttaacg cagcagttcg
    10021 aaaacaacag taattccaag taagcaatat tcatcgggag acaggtcatg tacgcggcaa
    10081 aaggcaccca ggcctatgca caaattggcg tcgaaagcgc cgtaatgagc gccagccagc
    10141 agcagctggt caccatgcta tttgatggag tgctgagcgc actggttaga gcgcgcctgt
    10201 ttatgcagga caacaatcag caaggcaaag gcgtctcttt gtcaaaagcg atcaacatta
    10261 ttgagaacgg actgcgggtg agtcttgatg aagagagcaa agacgaatta acccaaaact
    10321 tgattgctct ttatagctat atggtcaggc gtttgctgca agccaattta cgcaacgatg
    10381 tctccgcagt cgaagaagtg gaaacattaa tgcgcaatat tgccgatgcc tggaaagagt
    10441 cgttactctc cccttctttg attcaggacc cagtctgatg aaccatgcac cgcatttgta
    10501 tttcgcctgg caacaactcg tcgaaaaaag ccagctcatg ttacgcctgg caacggaaga
    10561 acaatgggac aaactcatcg ccagcgaaat ggcgtatgtg aatgcggtgc aggagattgc
    10621 acatctgact gaagagattg acccgtccac cacgatgcag gagcagctcc gcccgatgct
    10681 gcgcctgatt ctcgacaacg aaagcaaggt aaagcagtta ttacagattc ggatggatga
    10741 actggcgaaa ctggtcggtc agtcatcggt gcaaaaatcg gtgttaagtg cctatggcga
    10801 tcagggcggc tttgttctgg ctccgcagga taacctcttt tgaatctgaa tgagtcgatg
    10861 gctcgcgaat aatccgatta cggctacgct tctaatgttc cccttgaatg gagtcgaaga
    10921 atgcgtaatc ccacgctgtt acaatgtttt cactggtatt acccggaagg cggtaagctc
    10981 tggcctgaac tggccgagcg cgccgacggt tttaatgata ttggtatcaa tatggtctgg
    11041 ttgccgcccg cctataaagg cgcatcgggc gggtattcgg tcggctacga ctcctatgat
    11101 ttatttgatt taggtgagtt tgatcagaaa ggcagcatcc ctactaaata tggcgataaa
    11161 gtacaactgc tggccgccat tgacgccctg aaacgtaatg acattgcggt gctgttggac
    11221 gtggtagtca accacaaaat gggcgcggat gaaaaagaag ctattcgcgt gcagcgtgta
    11281 aatgctgacg accgtacgca aattgatgaa gaaatcattg agtgcgaagg ctggacgcgt
    11341 tacaccttcc ccgcccgtgc cgggcaatac tcacagttta tctgggattt caaatgtttt
    11401 agcggtatcg accatatcga aaaccctgat gaagatggca tatttaaaat tgttaacgac
    11461 tacaccggcg aaggctggaa cgatcaggtt gatgatgaat taggtaattt cgattatctg
    11521 atgggcgaga atatcgattt tcgcaatcat gccgtgacgg aagagattaa atactgggcg
    11581 cgctgggtga tggaacaaac gcaatgcgac ggttttcgtc ttgatgcggt caaacatatt
    11641 ccagcctggt tttataaaga gtggatcgaa cacgtacagg aagttgcgcc aaagccgctg
    11701 tttattgtgg cggagtactg gtcgcatgaa gtcgataaac tgcaaacgta tattgatcag
    11761 gtggaaggca aaaccatgct gttcgatgcg ccgctgcaga tgaaattcca tgaagcatcg
    11821 cgcatggggc gcgactacga catgacgcag attttcacgg gtacattagt ggaagccgat
    11881 cctttccacg ccgtgacgct cgttgccaat cacgacaccc aaccgttgca agccctcgaa
    11941 gcgccggtcg aaccgtggtt taaaccgctg gcgtatgcct taattttgtt gcgggaaaat
    12001 ggcgttcctt cggtattcta tccggacctc tacggtgcgc attacgaaga tgtcggtggt
    12061 gacgggcaaa cctatccgat agatatgcca ataatcgaac agcttgatga gttaattctc
    12121 gcccgtcagc gtttcgccca cggtgtacag acgttatttt tcgaccatcc gaactgcatt
    12181 gcctttagcc gcagtggcac cgacgaatat cccggctgcg tggtggtcat gtcgaatggg
    12241 gatgatggcg aaaaaaccat tcatctggga gagaattacg gcaataaaac ctggcgtgat
    12301 tttctgggga accggcaaga gagcgtagtg accgacgaaa acggcgaagc aaccttcttt
    12361 tgcaacggcg gcagcgtcag cgtgtgggtt atcgaagagg tgatttaaag tcatccccgg
    12421 cggcaagccg gggagatttc attacggcag tggcgtcggt aacggcgctt tatccagcgc
    12481 ggcggcacac tctgctgtcg ggcgatccac acgctccatc gtcataccat cgtactcaat
    12541 ggtgttaccg tcacgctcaa cctcgtacag ctcacgtttt acggtaacgt tagtgagatc
    12601 atcagacatc aatgtcaact tacccggcac ggcaatcacc cgctgccact gacggcaatc
    12661 cagggtatcg ccctctttag tgacaatcag gctgcctatc gcttccgggc tgaccagcgc
    12721 acgttgcggc cctttggtct gccagtagcc cgccagccag tccggcgcgg gggttttcac
    12781 gacattgtta tagttttcga cttcggcaca gcccgccagc aacatgagcg cgcctgcaat
    12841 tgcgagtttt ttcatcattc gtcctgcatg agaagaaaag aggattgtgg cattaaagcc
    12901 ctgatgtcgc cagcctttcc aggttcagag agaaattt
//
LOCUS       DACBVI010000125        12931 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.125, whole genome shotgun sequence.
ACCESSION   DACBVI010000125 DACBVI010000000
VERSION     DACBVI010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.125"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..168
                     /locus_tag="IU325_17285"
     CDS             <1..168
                     /locus_tag="IU325_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP5810754.1"
                     /translation="KGFGFITPDDGSKDVFVHFTAIQSNEFRTLNENQKVEFSIEQGQ
                     RGPAAANVVTL"
     gene            179..367
                     /locus_tag="IU325_17290"
     CDS             179..367
                     /locus_tag="IU325_17290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP5810755.1"
                     /translation="MTQHLHFHCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTEHAPLDFTK"
     gene            342..572
                     /gene="ymcE"
                     /locus_tag="IU325_17295"
     CDS             342..572
                     /gene="ymcE"
                     /locus_tag="IU325_17295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HAP5810756.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPETNTLLACCCLLRPEEVNSEY"
     gene            562..735
                     /locus_tag="IU325_17300"
     CDS             562..735
                     /locus_tag="IU325_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HAP5810757.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(784..1857)
                     /locus_tag="IU325_17305"
     CDS             complement(784..1857)
                     /locus_tag="IU325_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HAP5810758.1"
                     /translation="MTEKRRTRWQRRPGTTGGKLPWYDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVIVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1929..4673)
                     /gene="torS"
                     /locus_tag="IU325_17310"
     CDS             complement(1929..4673)
                     /gene="torS"
                     /locus_tag="IU325_17310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HAP5810759.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQHQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYGDLLALY
                     QQDNEISHRLQTLTQNNIAQFAQFSSEVSQLVDSIELRNQHGLAHLEKASARGQYSLL
                     LLGIVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATEIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGHIILRSRTDGELWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMSGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVHLDGL
                     RLLLIEDNPLTQRITVEMLNNSGAQVVAVGNAAHAIETLQNSEPFAAALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQPLDVSQLNEDAQLMGTEKIHEWLALFKQHALPLLDDIGIARSSQNSEKIK
                     RAAHQLKSSCSSLGMRSASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLNKKDLNAI
                     "
     gene            4756..5784
                     /gene="torT"
                     /locus_tag="IU325_17315"
     CDS             4756..5784
                     /gene="torT"
                     /locus_tag="IU325_17315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HAP5810760.1"
                     /translation="MRVLLLLLLSLFMLPGFSADNLLRWHDAQHFSVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPHVKSRVGVPWFQMGYQPGRYLVQ
                     WSHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5757..6449)
                     /gene="torR"
                     /locus_tag="IU325_17320"
     CDS             complement(5757..6449)
                     /gene="torR"
                     /locus_tag="IU325_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HAP5810761.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLTRQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6579..7751
                     /gene="torC"
                     /locus_tag="IU325_17325"
     CDS             6579..7751
                     /gene="torC"
                     /locus_tag="IU325_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HAP5810762.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAGK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7751..10297
                     /gene="torA"
                     /locus_tag="IU325_17330"
     CDS             7751..10297
                     /gene="torA"
                     /locus_tag="IU325_17330"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HAP5810763.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRASAAQAAT
                     EAVTSKEGILTGSHWGAIRATVKEGRFVAAKPFELDKYSSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPSVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            10294..10893
                     /gene="torD"
                     /locus_tag="IU325_17335"
     CDS             10294..10893
                     /gene="torD"
                     /locus_tag="IU325_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HAP5810764.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNELENRIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDNLRQKTLTA
                     LRQWLPEFAARCHQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="IU325_17340"
     CDS             complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="IU325_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HAP5810765.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="IU325_17345"
     CDS             complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="IU325_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HAP5810766.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFSRQFQHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            12530..>12931
                     /locus_tag="IU325_17350"
     CDS             12530..>12931
                     /locus_tag="IU325_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810767.1"
                     /translation="MSSNIHGISCTANNYLKEAWSNIKNEHEKNQTYSITLFENTLVC
                     FMRLYKEIRRQKAEDYIPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTKIYSGVSG
                     GMTYTIQYVQDIDVVRVSLPGRASESITDFKG"
BASE COUNT         3144 a         3277 c         3399 g         3111 t
ORIGIN      
        1 aaaggttttg gctttatcac tcctgatgat ggcagcaaag acgttttcgt ccatttcacc
       61 gccatccaga gcaatgaatt ccgcacgctg aacgaaaatc agaaagttga attttctatt
      121 gagcaggggc aacgtggtcc cgcggcagcg aacgttgtta cgctctaagg ttgccgttat
      181 tactcaacat ctccatttcc actgtccatg ttgtcatggt tcacagtacc gcacatcggc
      241 attcgatgtg acggagcgaa atcctttagg ggcaaagtgt attttttgta aatcgacgat
      301 gatcaccttt gataacgtcg cgctgcaaat acgcactgag catgcgccgc tggatttcac
      361 aaaataatat caggctccct cgtggagcct tttttatatc tgccttattt ttcttcaacg
      421 ctgtatgtat agtaagcgat aacctgttga ttattgaatc tttcggggag atggcttata
      481 acatttctta cctgaccagg gtaccggaaa ccaacacctt actggcgtgt tgctgtcttt
      541 taagaccaga agaggttaac agtgaatatt gaagagttaa aaaaacaagc cgaaacggaa
      601 atcgctgact ttatcgcgca aaaaatcgcc gagctgaaca agaatacagg gaaagaagtc
      661 tctgaaattc gcttcactgc acgagaaaaa atgaccgggc ttgaaagtta tgatgtcaaa
      721 atcaaaataa tgtgattttg tgaacatcac cccgtgcgag gtgatgttcc gcttgttgct
      781 aatttagtgc ccaatcattg gcgcttgcgg aattaagcgt cggtacaatt cttccggcac
      841 cgggctttgc catacacccg catacattgc gtaaccaatc accgcaaaca taatccccag
      901 aaccagtagc gtcattaacc agccagacaa cgcaaaggct tttttatttg ccgcaggttt
      961 ttgcagtgaa aaggtcaatg ttgaggctac cggacatgac tctacgcaag tcatacaacc
     1021 ggtacattcc acggttcgta cctgaattaa tttatcgacc gggatccgcg atgggcaatt
     1081 ttttgcgcat ttgccacaat cgatacaact ttcggcattg cgacgaatct taaacggtga
     1141 taatagcgaa accacgccca tcagcgcgcc atatgggcaa agataacgac accaggcatg
     1201 gcgaataaac aggctggcaa tcagcaaaac gatcacgctg attaatgtcg ctgtccccat
     1261 atggcgaaag aaatcgagca ttttaacgtc catcaccacg ctgtagggcg acaacataaa
     1321 atagtgaatc gcctgagcgg gcatcaataa cgcaatatag agaaaaaaac tcaacagcaa
     1381 atacttcacg ccgcgcagag gaatatccag ccagcgggga aggacacatt gccgaccaaa
     1441 cagtttgtta ccgagatcgc cgattaattc agaaagcgta ccaaccgggc ataaccatga
     1501 acaaaaggcc tttttgagta atagactgat gacgataaaa gcgaccaata acagcatcgc
     1561 ggcggcgtgg acggacggta actgacctgt tgcaaggcta tatttcagat tcatcagacc
     1621 ggcaatcggt agccagcctt cgatgcctcc tggcctggcg acaaatgtcg tactacctgc
     1681 cgtttcgtaa tagcgcaccc aataccagaa cgtgatggca atataaatat tcattgccaa
     1741 cagtaataat tgcgtcgctt tacgccaggt cgtggcattg cgccagtcat accacggtaa
     1801 tttgccgccc gtcgtgcctg gccgccgctg ccagcgggtt cttcttttct ctgtcatgat
     1861 tttgccagtc cgttaagttg cataccaaat gccactattc tagttgttct taactggctg
     1921 atattgattc aaatcgcgtt caggtctttc ttattcaacc atgcttccag agcggcaaca
     1981 ctgcgtgtaa tttcttcgtg tggaaggggg gcagataatg gctgctgctc cagttgtgcg
     2041 catagctggc tggcgctacg cattcccaga ctcgagcaac tgctttttag ctgatgtgcg
     2101 gcacgcttta ttttttcgct gttctggctg gagcgggcga tgccgatgtc atcgagaagc
     2161 ggcagggcat gttgtttaaa taatgccagc cattcgtgga tcttctccgt ccccatcaac
     2221 tgagcatctt cattgagttg cgatacatcc agcggttgat cgttattgac ttgcagttgg
     2281 agatagtgcg ccagtaactg accgagcact tcacgcggca ccggtttagg gataatcccg
     2341 cgaaataatg aactggtacg ctggcgcaac gtttcgtcga tgacatgagc gctaaagcca
     2401 atcaaaacca gcgacggata ttgctgtgcc agttgccggg caagcgtaat gccgtcgata
     2461 tcgggcagat caaaatccac cagtgcggca gcaaacggtt cgctattttg caacgtctct
     2521 attgcgtgcg cggcatttcc taccgcaacg acctgcgccc cactgttgtt cagcatctca
     2581 acggtaattc gctgggttag cgggttatct tcaattaaca gcaaacgtaa accgtccaga
     2641 tgcaccgcct gattgactgt ttttggcacg ggtgcagtgg caacacgtaa cggcaagcgt
     2701 aaacaaaaac agcttccaac ctccggcgtg ctggtggcgc tcagttcgcc gctcatcgcc
     2761 tgggccagac ggctactgat agtcagcccc agcccggtgc cgccgcgttt gccgctaacc
     2821 tgaacaaatg gctggaagat ttcagccagt ttcgcgggat caataccgca gccgctgtct
     2881 tccacttcga ccagccacag ctcgccatca gtgcgactac gcaggataat gtgcccttcg
     2941 tcggtgaaac gcagggcgtt gctcaacagg ttggttatga cctgacgaat acgccgcgga
     3001 tcgcccatta acgcagtcgg tacatcgtcg gcaatttctg ttgccaggcg aatcgggcga
     3061 cctttcaccc ttccgctcat taattgcagg gtactttcca gcagcgggcg cggttcaaag
     3121 ggttcatcgc tgaccgaaac attcttgccg cctgcttcga tagccgaata atcgagaata
     3181 tcgttgagga tggtcagcaa cgattcacca gagtcggtaa ttgcccgcaa atcatcacgc
     3241 tgggcattaa gtgcggggtt atctgccagc agttgcgcgg tgccaagaat accgtacagc
     3301 ggtgtgcgga tctcatggct catcgccgcc agaaacgccg attttgcctg gctggctttt
     3361 tctgcttccg ctcgtgcctg tcggtgttct atcaccagtt cctgcaattc agctgtacgc
     3421 gctttgacct gcgccgccag ctgttcgcgg tggcgattca gtgcatgaac actgctgcga
     3481 aacgcatcca tcagccgccc gatggtgtcc agctcccgca cgccagcggt ttccggaaaa
     3541 ggggagtcga tatcaccgtc cagtaagcgt tgcagcgcct gcgtttgttc ggcaagtgga
     3601 cgcgtgactg agcgataaac cacgcgccag aggatcagaa tcaatgcaca aagtgaaact
     3661 atccccagta ataacaggct gtattgcccg cgtgcactgg ctttttccag atgtgccagc
     3721 ccgtgctgat tgcgcagctc aatactgtcg accagctgac tgacttcgct actaaactgc
     3781 gcgaactggg cgatgttatt ttgcgtgaga gtttgcaggc gatgactgat ttcattgtcc
     3841 tgttgataca gcgccagcaa atcgccatat tggcttacgg tagttaacgt tgttgcgacc
     3901 tgagcacgaa ctcccggatc ttcaatgcgt atttgccgac gttgcagaat tttcaccgca
     3961 ttattgagct gcttttccag cgttggcgca tttttctgga tctgttccaa ccccagattc
     4021 atcaccattt gctgcacccg cagggcgcta aggcgcagtt cattcatctg gttaacatac
     4081 tcaagatcga tatcaatcag ccgatcgagt gcactttctg cggcctgacg ctgatgttgt
     4141 tcaatcaaat cgtaaatccc ggcctgggtc gctccggcgg aggttgccgc attattcgcc
     4201 tgaccttgcg ccaggcgtgc gatttcatcg gcggcggcga ctatctgctg actgagttgc
     4261 tgttgttgct ggcgcagttg caaacgctgc cccaccagct ccccttgttg acgtaacgaa
     4321 cgggaaatct cctgctcctg ttgttcaata gcggtggtat caaaaccttg ttcccgtaac
     4381 gcttgcagca acgcattaat cttcaggctt tgtgcggtga gcattcgccc ctgcgcctgc
     4441 cacatctttt cgttatcggc actggtcagg ttctgcgcgg cgaaaagttc ccaggcgctg
     4501 gcttcgctca actggcgcgc catattcatg gtaggaatca atgcctgagt gttgtctttt
     4561 tccacctggc tgataaagcg caggttgtac catcccacca gggtactggt cagggttaac
     4621 agcgccatca gggcaaagcc catccagagt cttcgggtca gggttaaatt cacggtcggt
     4681 gcacttaagg tgaaaaaggt tgagtcgcaa agcggaatgc atctagcata aagccttatt
     4741 attgatgagg ctatcatgcg cgtactgcta ttgttacttc tttccctttt catgttgcct
     4801 ggtttttcgg ctgataacct gttgcgctgg catgatgcgc agcatttctc tgtgcaagcc
     4861 tctacgccgc ttaaagccaa acgcgcatgg aaactgtgcg cgctttatcc cagcctgaaa
     4921 gattcatact ggttatcgtt gaactatggt atgcaggagg ctgctcgccg ctacggtgtg
     4981 gatttaaaag tgctggaggc aggcggctac agccagttgg ctacccagca agcacaaatc
     5041 gaccagtgta aacagtgggg cgcagaggcc attttgctcg gtagtagcac gacgtcattt
     5101 cccgatctgc aaaagcaggt agcaagtctg ccggtgatcg aactggtaaa tgctattgat
     5161 gctccccacg tgaaaagccg cgttggtgtg ccctggtttc agatgggcta tcaaccagga
     5221 cgatatctgg tgcaatggag ccacggtaaa ccactgaacg tgctgttgat gcccggaccc
     5281 gataacgccg ggggcagtaa ggagatggtc gagggttttc gcgcagccat tgccggaagc
     5341 ccggtacgta ttgttgatat tgcgcttggc gataacgata ttgaaatcca gcgtaacctg
     5401 ttgcaggaga tgctggagcg ccatccagaa atcgacgtcg ttgccggaac ggccattgcg
     5461 gcagaggcgg caatggggga agggcgtaat ctgaaaacac cgcttaccgt ggtgtcgttt
     5521 tatctttcac atcaggtgta tcgcgggctg aagcggggaa gagtgattat ggctgccagc
     5581 gatcaaatgg tctggcaggg ggaactggcg gttgagcagg ccatcaggca attacagggg
     5641 caatcggtgt ctgataatgt cagcccaccg attttagttc tgacgccgaa aaatgccgac
     5701 cgtgaacata tccgccgctc gctgtcacca gggggatttc gtccggtcta tttttatcag
     5761 cacacatcag cggctaagaa ataaccttca ccatgttgcg tcaccagtaa atccgcgctg
     5821 agtttatggc gtaaacgacg aattaacaca tcgacggtgc gcaggtcagg gttttccacc
     5881 cgacgcgcag agagcatacg cagcagacgt tcacggctaa gaatttcgcc cggattcgtc
     5941 acaaatgcca ccaacatttc atactctgcg cgggtcagtt taatcggctc gccatcccgc
     6001 tccagcgtat ggcgcgacac attcaggcaa taaccggcaa agcgatagca gttgtcctgt
     6061 gtgtgcggtt gagcttgtcg cgtgaggtcg attcgccaga gcagattttt cacccgtact
     6121 accagttcgc gcagttccag cggtttggtg acgtaatcgt ctgcgcccat ttccagccca
     6181 acaatacggt caatccgatc gctgcgtccc gtaaccagaa taatccccac cgttgagcgt
     6241 tctcgcaggg cgcgggtcaa catcaggcca ttttcatcgg gcaaattgat atccagcaga
     6301 attaaatcca ccggctgatt ctgcataatt tcccgcagcc cggcaccgct cgccgtaacg
     6361 gaaacggtat acccctcctg agtgaagtag gattgtaatc gcgcctgggt aaccggctca
     6421 tcttcaacaa taacaatgtg atgtggcatc agagggtttt actcattctg ttcatatctg
     6481 ttcatattct gccgtaagcc gttcatcctg accagtgccg ctgttcatat ttactcatta
     6541 agatcgcttc actaaaccat aattttacag gggctattat gcggaaactc tggaacgcgc
     6601 tacgccgacc cagtgctcgt tggtcggtac tggcgctggt cgctattggg attgtgattg
     6661 gcattgcgct gattgtattg ccacacgttg ggatcaaagt caccagcaca accgaatttt
     6721 gtgtcagttg ccacagtatg caaccggtgt atgaagaata taaacagtcg gtgcatttcc
     6781 agaatgcctc cggcgtgcga gctgaatgcc atgactgtca tatcccgccg gatattccag
     6841 gcatggtgaa gcgcaaactg gaagcgagca acgatatcta ccagaccttt attgcccact
     6901 ccattgatac acctgaaaaa ttcgaagcca aacgcgcgga acttgccgag cgtgaatggg
     6961 cgcgaatgaa agaaaacaac tcggcaacct gccgctcctg ccataactac gacgcgatgg
     7021 atcatgccaa gcagcatcct gaagcggcac gtcagatgaa ggtggcagcg aaagataatc
     7081 aatcctgcat cgactgtcat aaaggtattg cccaccagtt gccggatatg agtagtggct
     7141 tccgtaagca gttcgatgag ctgcgcgcca gtgctaatga cagtggtgac acgctgtact
     7201 ccattgatat taagccgatt tatgcgggga aaggcgataa agaagcctct ggttctctgc
     7261 tgcctgcttc ggaagtgaaa gtccttaaac gtgacggcga ctggctgcaa attgaaatta
     7321 ccggctggac ggaaagcgcc ggacgtcagc gtgttctcac ccagttccca ggtaaacgca
     7381 tctttgttgc ctcgattcgt ggtgatgtgc agcagcaggt gaaaacgctg gagaaaacta
     7441 ccgttgccga caccaatact gagtggagca aattgcaggc cactgcgtgg atgaagaaag
     7501 gcgacatggt aaacgatatc aaaccgatct gggcttatgc ggattcgttg tacaacggca
     7561 cctgtaacca gtgccacggc gcaccggaaa tctctcactt tgacgctaac ggttggatcg
     7621 gcacgctcaa cggcatgatt ggctttacca gcctcgataa acgtgaagaa cgcaccttgt
     7681 tgaaatatct gcaaatgaat gcgtctgaca ccgcaggtaa ggctcacggc gataagaagg
     7741 aagaaaaata atgaacaata acgatctctt tcaggcatca cgtcggcgtt ttctggcaca
     7801 actcggcggc ttaaccgtcg ccgggatgct ggggccgtca ttgttaacgc cgcgccgtgc
     7861 gtctgcggcg caagcggcga ctgaggctgt cacctcgaaa gagggcattc ttaccgggtc
     7921 gcactggggg gctatccgcg cgacggtgaa ggagggtcgc tttgtggcgg caaaaccgtt
     7981 cgaactggat aaatattcgt cgaaaatgat cgccggattg ccggatcacg tacacaacgc
     8041 ggcgcgtatt cgttatccga tggtacgcgt ggactggcta cgtaagcgcc atctgagcga
     8101 cacctcccag cgcggtgata accgttttgt gcgcgtgagc tgggatgaag ccctcgacat
     8161 gttctatgaa gagctggaac gtgtgcagaa aacgcacggg ccgagtgcct tgctgaccgc
     8221 cagtggttgg cagtcgacgg ggatgttcca taacgcttcg gggatgctgg cgaaagcgat
     8281 tgccttgcat ggtaatagcg ttggtacggg cggagattac tctaccggtg ctgcgcaggt
     8341 gatcctgccg cgcgtagtcg gttcgatgga agtgtatgaa cagcaaacct cctggccgct
     8401 ggtattgcag aacagcaaaa ccattgtgct gtggggctcc gatttgctga aaaaccagca
     8461 agcgaactgg tggtgcccgg atcacgatgt ttatgaatat tacgagcagc taaaagcgaa
     8521 agtcgccgcc ggtgaaattg aggtcatcag catcgatccg gttgtcacat ccacccatga
     8581 gtatctgggg cgcgagcatg tgaagcacat tgcggttaac ccgcaaactg acgtgccgct
     8641 gcaactggcg ctggcgcata cgctgtacag tgaaaacctg tacgacaaaa acttcctcgc
     8701 taactactgt gtaggttttg agcagttcct gccgtatctg ctgggtgaga aagacggtca
     8761 gccgaaagat gccgcatggg ctgaaaaact gaccggcatt gatgccgaaa ccattcgtgg
     8821 gctggcgcgg cagatggcgg cgaacagaac gcaaattatt gctggctggt gcgtgcagcg
     8881 tatgcagcac ggtgaacagt gggcgtggat gattgtcgtt ctggcggcga tgctggggca
     8941 aattggcctg ccaggtggtg gctttggttt tggctggcac tataacggcg caggcacgcc
     9001 ggggcgtaaa ggcgttattc tgagtggttt ctccggctct acgtcgattc cgtctgttca
     9061 cgacaacagt gactacaaag gttacagcag caccattccg attgcccgtt ttatcgatgc
     9121 gatcctcgaa ccggggaaag tgatcaactg gaacggtaaa tcggtaaaac tgccgccgct
     9181 gaaaatgtgt atttttgccg gaactaaccc gttccatcgc catcagcaga tcaaccgcat
     9241 tattgaaggc tggcgtaagc tggaaacggt tatcgccata gataaccagt ggacctcaac
     9301 ctgccgcttt gccgatatcg tactgcctgc gaccacgcag tttgagcgta acgatctcga
     9361 ccagtacggc aaccactcca accgtggcat tatcgccatg aaacaggtcg tgccgccgca
     9421 gttcgaggcg cgcaacgact tcgatatttt ccgcgagctg tgccgtcgct ttaatcgcga
     9481 agaagccttt accgaagggc tggacgaaat gggctggctg aaacgcatct ggcaggaagg
     9541 tgtacaacag ggcaaaggac gcggcgttca tctgccagcg tttgatgact tctggaataa
     9601 caaagagtat gtcgagtttg accatccgca gatgtttgtt cgtcaccagg cattccgcga
     9661 agatccggat ctcgaaccgc tgggtacgcc gagtggcctg attgagatct actcgaaaac
     9721 catcgccgat atgaactacg acgattgtca ggggcatccg atgtggtttg agaaaatcga
     9781 acgctcccac ggcgggcccg gctcgcagaa gtatccgttg catctgcaat ctgtgcatcc
     9841 ggatttccga cttcactcgc agttatgtga gtcggaaacg ctgcgtcagc aatatacggt
     9901 agcgggcaaa gagccagtgt tcattaaccc gcaggatgcc agcgcgcgtg gtattcgtaa
     9961 cggtgatgtg gtacgcgtct ttaacgctcg tggccaggtg ttggctggag cagtggtttc
    10021 tgaccgctat gcacccggcg tggcgcgaat tcatgaaggg gcatggtacg atccagataa
    10081 aggcggcgag cctggtgcgc tgtgcaaata cggtaacccc aacgtgctga ccatcgacat
    10141 cggtacatcg cagctggcgc aggcgaccag tgcgcacact acgctggtgg aaattgagaa
    10201 gtacaacgga acagtggagc aagtgacggc gtttaacggc cccgtggaga tggtggcgca
    10261 gtgcgaatat gttcccgcgt cgcaggtgaa atcatgacca cgctgacagc acaacagatt
    10321 gcctgtgttt acgcctggct ggcgcagttg ttctcccgtg aactggacga tgaacaactg
    10381 acgcaaatcg ccagtgcgca gatggctgaa tggttttcgt tgctgaaaag tgaaccgccg
    10441 ctcactgcgg cggtgaatga gctggaaaac cgtattgcca cgctgacagt acgtgacgat
    10501 gcccgtctgg aactggccgc tgacttttgc ggtctgtttc tgatgaccga caaacaagcg
    10561 gcgctgcctt atgcatcggc ctacaaacag gacgagcaag agattaaacg cttgttagtt
    10621 gaggcaggga tggaaaccag cggcaatttc aacgaaccgg cagatcatct ggcgatctat
    10681 ctcgagttgt tgagtcatct gcatttttcg ctgggagagg ggaccgttcc tgcgcgaaga
    10741 atcgacaatt tgcggcaaaa aacactgacg gcgctgcgac aatggttacc agagtttgct
    10801 gcgcgttgcc atcagtacga cagtttcggt ttttacgcgg cactaagcca gttattgctg
    10861 gtgttagtgg aatgcgacca ccaaaacaga taacatcgtt tgttggcctg ataagacgcg
    10921 tttagcgtcg catcaggcat tatggcgcag ttgccggatg cggcatgaac gtcttatccg
    10981 gcccacagga actgtaatcg ttgtagaccg gctaagatgc gtcatcgcat ccggcaaaca
    11041 cacatcacgg atgagctaca aaccgggaaa gccgctggcg cagcaggcgg ttttcctgct
    11101 tcaggtgcgc aatatcatcc attaacgtca gcgccaccgc gatccccggc cagtccagag
    11161 cgagttcatg acgcaggcgt accgcgcgtt gcaccacaat ggcggcatgg tcgtcaaata
    11221 cccaggttgt ttcctggatc tcacgcggtt caaccacccc caaaccgaca atttcattta
    11281 gctcctcttc agagatgccg gtatgcaggc aaaattcggt aatagtaaaa gtcaccgtaa
    11341 cattagccat tatgctttcc cccaatcttt acgtggatca aaagacgact gggcgtctgc
    11401 cagttgctgc catagcgcgg cagtgttttc atccggtttc ggcggcatca cgattttcag
    11461 taccgcatac agatcgccgg tctgtttttt gctcaccaga cctttgcctt taacgcgcaa
    11521 tcgttgccct gcctggctgc caggcgggat agtcagcaaa atgctttctt tcagtgttgg
    11581 aacggtgact ttagcaccca gcgccgcttc ccacgggcta accggcacca caatttccag
    11641 atcctggccg acaatatcaa acagcggatg tggcgcaata tgaatcacca gccacaaatc
    11701 gccattcggg ccgccatttt cgccaggcgt cccctggcct ttcaggcgga tgcgttgacc
    11761 attgccgacg cccgccggga tcttcacatt cagcgttttc gggatttcct gttcgatcat
    11821 gccaaaggcg ttataaaccg gcaggttata gctgatggta cgcttatgct cagtaagcgt
    11881 ttcttcgagg aataccgcca cttcgatttc aatatcgtgg ccgcgtgtgg cggggcgttg
    11941 acggctctgg cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa aatcttcggc
    12001 gttaaaactc tgaccgtcgc catgctggaa ctgacggcta aattgcggat cgttgcgatg
    12061 ttgccacatc tgatcatact cagcgcgacg ttgttcatca cttaacactt cccaggcttc
    12121 agcgacctct ttgaagcggg cttcggcatc cggttctttg ctgacatcag gatggtattt
    12181 gcgggccagt cgacgatagg cggtcttgat tgtcttgaga tcgtccgtcg gtttcacgcc
    12241 catgatggcg taataatcct ttaattccat agcgttatct cgcgtaaatc aactcaaatt
    12301 gaaggaaccc ctgtaaggta actcctataa gtgtagggta atcctcaaaa tttcatatgc
    12361 caacacagaa tatgttattg aaatcatcgc ggagaggagg tcgcctcaag gtggggtgtt
    12421 gaacatattt taaacaggtg aaaaagggtg agcgattttt gatagttgaa acaggcactt
    12481 taagtttaac tggggcgtca ttatttatta aattttatag gcgctatata tgagtagtaa
    12541 tatacatgga attagttgca ctgcaaataa ttatttgaaa gaagcatgga gcaatataaa
    12601 aaatgagcac gaaaaaaatc aaacatattc aatcacgctt tttgaaaaca cactggtgtg
    12661 ttttatgcgg ttgtataaag aaatcagacg tcaaaaagct gaagactaca ttccatgcct
    12721 ggaatgcgaa tcactagaaa aagaatttga ggaaatgcag aatgataatg atctatcatt
    12781 atttatgaga acattgcgta ctaatgatac aaaaatttat tcaggggttt caggaggtat
    12841 gacatatact atacaatatg ttcaagatat tgatgttgtt agagtgtcct tgccgggcag
    12901 agcttcagag tctatcacag attttaaagg t
//
LOCUS       DACBVI010000126        12917 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.126, whole genome shotgun sequence.
ACCESSION   DACBVI010000126 DACBVI010000000
VERSION     DACBVI010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.126"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            188..523
                     /locus_tag="IU325_17355"
     CDS             188..523
                     /locus_tag="IU325_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HAP5810768.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            683..1126
                     /gene="yjdN"
                     /locus_tag="IU325_17360"
     CDS             683..1126
                     /gene="yjdN"
                     /locus_tag="IU325_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HAP5810769.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AKNCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTH"
     gene            1259..2047
                     /gene="phnC"
                     /locus_tag="IU325_17365"
     CDS             1259..2047
                     /gene="phnC"
                     /locus_tag="IU325_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HAP5810770.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSYFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            2072..3088
                     /gene="phnD"
                     /locus_tag="IU325_17370"
     CDS             2072..3088
                     /gene="phnD"
                     /locus_tag="IU325_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP5810771.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKGNKGLNEQDKLAKTSAIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3149..3928
                     /gene="phnE"
                     /locus_tag="IU325_17375"
     CDS             3149..3928
                     /gene="phnE"
                     /locus_tag="IU325_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HAP5810772.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            3949..4674
                     /gene="phnF"
                     /locus_tag="IU325_17380"
     CDS             3949..4674
                     /gene="phnF"
                     /locus_tag="IU325_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HAP5810773.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4675..5127
                     /gene="phnG"
                     /locus_tag="IU325_17385"
     CDS             4675..5127
                     /gene="phnG"
                     /locus_tag="IU325_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HAP5810774.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRATVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5124..5708
                     /gene="phnH"
                     /locus_tag="IU325_17390"
     CDS             5124..5708
                     /gene="phnH"
                     /locus_tag="IU325_17390"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HAP5810775.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVNQPELATFAVTDEAIS
                     SEQLNALSSGTAVAPEAGATLILQVVSLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5708..6772
                     /gene="phnI"
                     /locus_tag="IU325_17395"
     CDS             5708..6772
                     /gene="phnI"
                     /locus_tag="IU325_17395"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HAP5810776.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGNTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSAQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLITECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6765..7610
                     /gene="phnJ"
                     /locus_tag="IU325_17400"
     CDS             6765..7610
                     /gene="phnJ"
                     /locus_tag="IU325_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HAP5810777.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWNEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            7607..8365
                     /gene="phnK"
                     /locus_tag="IU325_17405"
     CDS             7607..8365
                     /gene="phnK"
                     /locus_tag="IU325_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HAP5810778.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPSNRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8476..9156
                     /gene="phnL"
                     /locus_tag="IU325_17410"
     CDS             8476..9156
                     /gene="phnL"
                     /locus_tag="IU325_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HAP5810779.1"
                     /translation="MINVQNVSKTFILHQQNGVHLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGEEWVDLVTAPARKVVEIRKTTIGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            9153..10289
                     /gene="phnM"
                     /locus_tag="IU325_17415"
     CDS             9153..10289
                     /gene="phnM"
                     /locus_tag="IU325_17415"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HAP5810780.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRQPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRRHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLTQAVKLVTKN
                     PAQALNLQDRGVIGEGIRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10289..10846
                     /gene="phnN"
                     /locus_tag="IU325_17420"
     CDS             10289..10846
                     /gene="phnN"
                     /locus_tag="IU325_17420"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HAP5810781.1"
                     /translation="MTGKLIWLMGASGSGKDSLLTELRQREQTQLLVAHRYITRAASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGIGVEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            10833..11267
                     /gene="phnO"
                     /locus_tag="IU325_17425"
     CDS             10833..11267
                     /gene="phnO"
                     /locus_tag="IU325_17425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HAP5810782.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNIGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11269..12027
                     /gene="phnP"
                     /locus_tag="IU325_17430"
     CDS             11269..12027
                     /gene="phnP"
                     /locus_tag="IU325_17430"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HAP5810783.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDQIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTMEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNEVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(12186..12515)
                     /gene="yjdP"
                     /locus_tag="IU325_17435"
     CDS             complement(12186..12515)
                     /gene="yjdP"
                     /locus_tag="IU325_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HAP5810784.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVNDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12584..12901)
                     /locus_tag="IU325_17440"
     CDS             complement(12584..12901)
                     /locus_tag="IU325_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HAP5810785.1"
                     /translation="MQPVFFIKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEGIDKET
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
BASE COUNT         2817 a         3888 c         3642 g         2570 t
ORIGIN      
        1 atcttaaata ataaatggag gcaaaaagat tattttcttt ttgcgtttcc tttcaaatga
       61 aaacgatcgt cgtctaaaat cagcagtacc cccgacaaac tcagggattt tgtgtataat
      121 tgcggccttt ttcggcaatc tgccgttttt tggcgctttt gccctgctga cttttgagga
      181 aatccacatg tcattaccac actgcccaaa atgcaactcc gaatacacct acgaagataa
      241 cggcatgtac atctgcccgg aatgtgccta cgaatggaac gacgcagaac ctgcacagga
      301 aagcgacgag ctgatcgtta aagatgctaa cggcaatctg ctggctgacg gcgacagcgt
      361 taccatcatt aaagatctga aggtgaaagg tagctcttcg atgctgaaaa ttggcaccaa
      421 agtgaaaaac atccgcctgg ttgaaggcga ccataacatc gattgcaaaa tcgacggttt
      481 tggtccgatg aaactgaaat ctgagtttgt gaaaaagaac tgattgtatt gtgatcggta
      541 agccggataa ggcgctcgcg ccgcatccgg caacgatgcc gactgcctga tgcgacgctg
      601 accgcgtctt atcaggcttc cctctcatct gtataaattt cgaactacac ttaagtggct
      661 tctcttaact gaggtcacca tcatgccgtt aagtccctac ctctcttttg ccggtaactg
      721 tgccgacgca attgcttatt atcaacgtac gttgggcgct gaactgctct ataaaatcag
      781 cttcggcgaa atgccaaaat cagcgcagga cagcgccaag aactgccctt ccggaatgca
      841 atttcccgat accgccatcg cccacgccaa tgtgcgcatt gccggaagcg atatcatgat
      901 gagcgatgct ataccgtcag gaaaagccag ctactccggc tttacgctgg tgctcgattc
      961 gcaacaggtc gaagaaggaa aacgctggtt tgacaacctt gccgctaacg gaaaaatcga
     1021 aatggcctgg caggaaactt tctgggcgca tggcttcggc aaagtcaccg ataaatttgg
     1081 cgtaccgtgg atgattaatg tcgtcaaaca acaaccaacg cactaacccg ccgggagggc
     1141 cgccctcccg cactgtcatc gaaatctcgt taactcttca tccgttagtc acttttagtt
     1201 aacaaaatcg tcacaataat ccgccacgat ggagccactt ttttagggag gctgcatcat
     1261 gcaaacgatt atccgtgtcg agaagctcgc caaaaccttc aatcagcatc aggcgctgca
     1321 cgcggttgat ctgaacattc atcacggtga aatggtggct ctgcttgggc cgtcgggttc
     1381 cggcaaatcc acccttttac gtcacttaag cggtttgatt accggcgata aatctgccgg
     1441 tagccatatc gagctgctgg gccgcacagt ccagcgcgaa ggccgcctgg cccgcgatat
     1501 ccgcaaaagc cgcgccaaca ccggctacat cttccaacaa ttcaacctgg tgaatcgcct
     1561 gagcgtactg gagaacgtgc tgattggcgc gctcggcagc acgccattct ggcgcacctg
     1621 ttttagctac ttcactcgcg agcagaaaca gcgcgcgtta caggcgctga cccgcgttgg
     1681 catggtgcac tttgcccatc agcgcgtttc caccctctcc ggcggacagc agcagcgtgt
     1741 ggcgattgcc cgtgcgctga tgcagcaggc gaaggtgatt ctggccgatg aacccatcgc
     1801 ctcgctggac ccggaatcag cgcgcatcgt gatggacacc ctgcgcgaca tcaaccagaa
     1861 cgacggcatc accgtggtcg tcacgctgca tcaggtggat tacgccctgc gctactgcga
     1921 acgcatcgtc gccctgcgtc aggggcacgt cttctacgac ggcagcagcc aacagtttga
     1981 taacgaacgc tttgatcatc tctatcgcag catcaaccgc gtcgaagaga acgcgaaagc
     2041 tgcctgacat ccccatcatt gaggaaaacg aatgaacgct aagataattg cctcgctggc
     2101 cttcaccagc atgttcagcc tcagcaccct gttaagcccg gcgcacgccg aagagcagga
     2161 aaaggcgctg aatttcggca ttatttcaac ggaatcacag caaaacctga aaccgcaatg
     2221 gacgccgttc ttacaggata tggagaagaa gctgggcgta aaggtcaacg ccttctttgc
     2281 cccggactac gcgggcatta tccaggggat gcgcttcaat aaagtggata tcgcctggta
     2341 cggcaatctg tcggcgatgg aagcggtgga tcgcgccaac ggtcaggtct tcgcccagac
     2401 ggtcgcagcg gatggatcgc cgggttactg gagcgtgttg atcgtcaaca aagacagtcc
     2461 gatcaacaac ctgaacgatc tgctggcgaa gcgtaaagat ctcaccttcg gcaatggcga
     2521 tcctaactcc acctctggct tcctcgtccc cggctactac gtcttcgcca aaaacaatat
     2581 ctccgccagc gacttcaagc gcaccgtcaa cgccgggcat gaaaccaacg cgctggccgt
     2641 cgccaacaag caggtggatg tggcgaccaa caacaccgaa aacctcgaca agctgaaaac
     2701 ctccgcgcca gagaagctca aagaactgaa ggtgatctgg aagtcgccgc tgatcccagg
     2761 cgatccgatc gtctggcgca agaatctttc cgaaaccacc aaagacaaga tctacgactt
     2821 ctttatgaat tacggcaaaa cgccggaaga gaaagcggtg ctggaacgcc tgggctgggc
     2881 cccgttccgc gcctccagcg acctgcaact ggtgccgatt cgccagctcg cgctgtttaa
     2941 agagatgcag ggcgtgaaag gcaataaagg gctgaatgag caggacaagc tggcaaaaac
     3001 tagcgccatt caggcgcaac tggatgacct ggaccgcctg aacaacgcgt taagcgcgat
     3061 gagttcggtg agtaaagcgg tgcagtaatg tgctcgatcg gtcccctctc ccacagggag
     3121 agggagaaaa cattaaacgg agcaatatat gcaaaccatc accatcgccc cacccaagcg
     3181 cagctggttc tcgcttctga gctgggccat tgttctcgcc gtgctggtcg tctcgtggca
     3241 gggcgcggaa atggccccgc tcacgctgat taaagacggc ggcaacatgg cgacgttcgc
     3301 tgccgacttc ttcccgcccg atttcagcca gtggcaggat tacctcaccg aaatggccgt
     3361 cacgctgcaa atcgccgtct ggggcaccgc gctggcggtg gttctctcca tccccttcgg
     3421 tctgatgagc gccgaaaacc tggtgccgtg gtgggtttac cagcccgtac gccgcctgat
     3481 ggacgcctgc cgcgccatta acgaaatggt cttcgccatg ctgttcgtgg tcgccgtcgg
     3541 ccttggcccg ttcgctggcg tgctggcgct gtttatccac accaccggcg tgctctccaa
     3601 gctgctttcc gaagcggtgg aagcgattga acccggcccg gtggaaggca ttcgcgccac
     3661 cggtgccaac aagctggaag agatcctcta cggcgtgctg ccacaggtga tgccgctgct
     3721 gatctcctac tccctctatc gcttcgaatc caacgtccgt tcggccaccg ttgttggcat
     3781 ggtcggcgct ggcgggatcg gcgtcaccct gtgggaagcg attcgcggtt tccagttcca
     3841 gcaaacctgc gccctgatgg tgctgattat cgtcacggtc agcctgctgg atttcctctc
     3901 tcaacggttg cgtaagcact ttatctgata agcgaggcat tgatatctat gcacttgtct
     3961 acacatccga ccagctaccc aacacgctat caggagatag ccgcaaaact tgagcaggag
     4021 cttcgtcaac actaccgctg cggcgactat cttcccgccg agcagcaact ggcggcgcgc
     4081 tttgaggtga accgccacac cctgcgccgc gccatcgacc aactggtgga aaaaggctgg
     4141 gtacagcgcc gtcagggcgt cggcgtgctg gtgctgatgc gcccgttcga ttacccgctc
     4201 aacgcccagg cgcgttttag ccagaacctg ctggatcagg gcagccatcc caccagcgaa
     4261 aaactgctgt ctgtgctgcg cccggcctcc ggtcacgtcg ctgacgcgct ggggataacc
     4321 gagggcgaga acgttatcca cctgcgcacc ctgcgccggg tcaacggcgt cgcgctctgt
     4381 ttaatcgacc actactttgc ggacctcacc ctctggccga cgctgcaacg cttcgacagc
     4441 ggctcgctgc acgattttct gcgcgagcaa acggggattg cgctgcgccg cagccagacg
     4501 cggatcagcg cccgccgcgc ccaggccaaa gagtgccagc gtcttgaaat cccgaatatg
     4561 tcgccgctgc tgtgcgtgcg cacccttaac caccgtgacg gtgaaagcag cccggcggag
     4621 tactccgtca gcctgacgcg cgccgacatg attgaattca ctatggagca ctgaatgcac
     4681 gcagataccg cgactcgcca gcactggatg tccgtactgg cacacagcca accggctgaa
     4741 ctggcagccc gcctgaaggc gctgaacatc actgccgact atgaagtaat ccgcgccgct
     4801 gaaaccggcc tggtgcagat tcaggcgcgg atgggtggca ccggcgaacg cttttttgct
     4861 ggcgacgcca cgctcacccg cgccaccgta cgcctgactg acggcacgct cggctacagc
     4921 tgggtgctgg ggcgcgacaa acagcacgcc gaacgctgcg cgctgattga cgcgctgatg
     4981 cagcaaaccc gccattttca aaacttatca gaaaccctta ttgccccgct ggacgctgac
     5041 cgtatggcac gcattgccgc acgccaggcc gaagtgaacg ccagccgggt cgacttcttt
     5101 acgatggttc gcggagataa cgcatgaccc tggaaaccgc ttttatgctt cctgtgcagg
     5161 atgcccagca cagttttcgt cgcctgttaa aggccatgag cgagccgggc gtgattgtcg
     5221 ccctgcatca gctcaaacgc ggctggcagc cgctgaatat cgccaccacc agcgtgctgc
     5281 tgacgctggc cgataacgac acgccggtgt ggctttctgc gccattaagt aacgatatcg
     5341 tcagccagag cctgcgtttt cataccaacg cgccgctggt caatcagccg gaactggcga
     5401 ccttcgcggt aacggatgag gcgatttcca gcgaacagct taacgccctt tccagcggca
     5461 ccgctgttgc gccagaagcg ggcgcgacgc tgattttaca ggtcgtcagc ctgagcggcg
     5521 ggcgcatgct gcgccttact ggcgcgggta ttgccgaaga acgaatgatc gctccgcagc
     5581 tgccggagtg cattctgcac gaacttaccg agcgcccgca cccgttcccg ctcggcatcg
     5641 acctgatcct gacctgcggc gagcgcctgc tggctatccc gcgaaccact catgtggagg
     5701 tgtgctgatg tacgttgccg tgaaaggggg cgaaaaggcg atcgacgccg cccacgccct
     5761 gcaagagagc cgacgccggg gcaataccga tttgcctgaa ctgagcgtcg cccagattga
     5821 acagcagctt aatcttgcag tagatcgcgt gatgaccgaa ggcggcattg ccgaccgcga
     5881 actggcggcg ctggcgctga aacaggccag cggcgataac gtcgaagcga ttttcctgct
     5941 acgcgcctac cgcaccacgc tggcgaagct ggcggtaagc gagccgctcg acaccacgga
     6001 gatgcgtctc gaacggcgta tttcagcggt ttataaagat attcccggcg gccagctgct
     6061 tggcccaacc tacgactaca cccatcgcct gctcgatttc accctgctgg caaacggcga
     6121 agcgccgacg ctgaccaccg ccgacagcgc acaacagccg tcgccgcacg ttttcagcct
     6181 gctggcgcgt caggggctgg cgaagtttga agaggatagc ggcgcacagc cggatgacat
     6241 cacccgcacg ccgccggttt acccctgctc acgttcttcc cgtttgcagc agctcatgcg
     6301 cggagacgaa ggctatttgc tggcgctggc ctactccacc caacgcggtt acggacgcaa
     6361 tcatccgttc gcaggcgaaa tccgcagcgg ctatatcgac gtgtcgattg tgccggaaga
     6421 gctgggattt gcggtgaacg tcggcgaact gctgataact gagtgtgaaa tggtcaacgg
     6481 ttttatcgac ccgccggatg agccgccgca cttcacgcgc ggctacgggc tggtgttcgg
     6541 catgagcgag cgcaaagcga tggcaatggc gctggttgac cgcgctctac aagccccgga
     6601 gtacggcgag cacgcgacag gcccggcaca ggatgaagag ttcgtgctgg cgcatgccga
     6661 caacgtcgaa gccgcaggct ttgtctcgca cctcaaactc ccccactacg tcgatttcca
     6721 ggccgaactg gagctactca aacgtctgca acaggagcag aaccatggct aatctgagcg
     6781 gctacaactt tgcttacctc gacgagcaga ccaaacgcat gatccgccgc gccatcttaa
     6841 aagcggtggc gatccccggt tatcaggtgc cgtttggcgg gcgcgagatg ccgatgccat
     6901 acggctgggg aactggtggt atccagctta ccgccagcgt gattggcgaa agcgacgtgt
     6961 tgaaggtgat tgaccagggt gcggatgaca ccaccaacgc cgtgtcgatt cgcaacttct
     7021 ttaagcgcgt gaccggggta aacaccactg aacgtacgga cgatgcgacg cttatccaga
     7081 cgcgtcaccg catccccgaa acgccgctga ccgaagatca gatcattatc ttccaggtgc
     7141 caatcccgga accgctgcgc tttatcgagc cgcgcgaaac ggagacccgc actatgcacg
     7201 cgctggagga gtacggcgtg atgcaggtga aactgtatga agatatcgcc cgcttcggtc
     7261 atatcgccac cacctatgct tatccggtga aggtgaacgg gcgctacgtg atggacccgt
     7321 cgccgatccc gaaattcgat aacccgaaaa tggacatgat gcccgccctg cagctgttcg
     7381 gcgcagggcg cgagaagcgc atctatgcgg tgccgccgtt tacccgcgtg gaaagcctcg
     7441 atttcgacga tcacccgttc accgttcagc agtggaatga gccatgcgcc atctgcggat
     7501 cgacccacag ctatcttgat gaagtggtgc tggatgacgc cggaaaccgc atgtttgtct
     7561 gctccgatac cgattattgc cgccaacaga gcgaggcaaa aaaccaatga atcaaccgtt
     7621 actttcggtc aataacctga cccaccttta cgcgccgggc aaaggcttta gcgatgtctc
     7681 ttttgattta tggccggggg aagtgctggg catcgtcggg gaatccggct ccgggaagac
     7741 cacgctgctg aaatcaattt ccgcgcgtct gacgccgcag caaggggaga ttcactatga
     7801 aaaccgttcc ctctatggca tgagcgaggc cgaccgccgt cgcctgctgc gcaccgaatg
     7861 gggcgtggtg catcagcatc cactcgacgg cctgcgccgc caggtgtcgg caggcggcaa
     7921 tatcggcgag cggctgatgg cgaccggggc gcgtcattac ggcgatattc gcgccaccgc
     7981 gcagaagtgg ctggaggagg tggagattcc ctccaaccgg atcgacgacc tgccgaccac
     8041 cttttccggc ggtatgcagc aacgtttgca gattgcccgc aacctggtga cgcatccgaa
     8101 gctggtgttt atggatgaac cgaccggcgg gctggatgta tcggtacagg cgcgcctgct
     8161 cgatctgctg cgcggtctgg tggtggagct gaatctggcg gtggtgattg tcacccacga
     8221 tctgggcgtc gcccgcttgc tggcggaccg tttgctggtg atgaagcagg ggcaagtagt
     8281 ggagagtggg ttaaccgacc gcgtgctcga cgaccctcac catccatata cccagctgct
     8341 ggtgtcatcg gttttgcaga attgagccag tgccggatac ggcgtaaacg ccttatccgg
     8401 ccgacaaatg cgctccccgt aggtcggata agacgcgtca gcgtcgcatc cgacacccga
     8461 acaacgaggc gaaaaatgat taacgtacaa aacgtcagta aaaccttcat cctgcaccag
     8521 caaaacggcg tgcacctgcc cgtcctcaac cgtgcctcgc tcaccgtcaa cgcaggcgaa
     8581 tgcgtggtgc ttcacggcca ctccggcagc ggaaaatcaa ccctgctgcg ctcgctgtac
     8641 gccaactatc tgcccgacga agggcaaatc cagatcaaac acggtgagga atgggtagac
     8701 ctggtcaccg cgccagcccg caaagtggtg gagatccgca aaaccaccat cggctgggtg
     8761 agccagtttc tgcgcgtcat cccgcgtatt tcggcactgg aagtggtgat gcagcctctg
     8821 ctcgataccg gcgtaccgcg tgaagcctgc gccgctaaag ccgcgcgtct gctcacccgc
     8881 ctgaacgtgc ctgaacgcct gtggcacctg gcaccatcga ccttctccgg cggcgaacag
     8941 cagcgcgtca acatcgcccg tggctttatc gtcgactatc cgattctgct gctcgacgaa
     9001 cctaccgcct cgctggacgc taaaaacagc gccgccgtag tggaactgat ccgcgaagcc
     9061 aaagcccgtg gcgcggccat cgtcggtatc ttccatgacg aagccgtacg caatgacgtc
     9121 gccgaccgcc tgcacccgat gggagcctct tcatgattat caataacgtc aaactggtgc
     9181 tggaaaatga agtggtgcac ggttcactgg aagtacagga tggcgaaatc cgcgcctttg
     9241 ccgaaagcca gagccgccag ccggaagcga tggacggcga aggtggctgg ctactgccgg
     9301 ggctgattga gctgcatacc gataatctgg ataaattctt caccccgcgc ccgaaggtcg
     9361 actggcccgc ccactcggcg atgagcagcc acgatgcgct gatggtggcg agcggcatca
     9421 ccaccgtgct ggacgcggtg gcgattggcg acgtgcgcga cggcggcgat cggctggaga
     9481 atctggagaa gatgatcaac gccattgaag agacgcagaa acgcggcgtc aaccgcgccg
     9541 agcaccgcct gcacctgcgc tgcgaactgc cgcatcacac cacgctgcca ctgtttgaaa
     9601 aactggtgca gcgcgagccg gtgacgctgg tgtcgctgat ggatcactcg ccgggacagc
     9661 gccagttcgc caaccgcgag aagtatcgcg aatattatca gggcaaatac tcgctcaccg
     9721 atgcgcagat gcagcagtac gaagaagagc aactggcgct tgccgcacgc tggtcacagc
     9781 ctaatcgcga atccatcgcc gccctgtgcc gcgcgcgaca aattgccctc gccagccacg
     9841 atgacgccac gcacgcccac gttgccgaat cccaccagct tggcagcgtg atcgccgaat
     9901 ttcccaccac gtttgaagcg gcggaagctt cgcgcaggca tggtatgaac gtgctgatgg
     9961 gcgcgccgaa tatcgtgcgc ggcggttcgc actccggcaa cgtggcggcc agtgaactgg
    10021 cgcagcttgg cctgctggat atcctctctt ccgactacta ccccgccagc ctgctcgatg
    10081 cggcatttcg cgtcgccgac gacgagagca accgctttac gctgacgcag gcggtgaagc
    10141 tggtgaccaa aaatccggcc caggcgctca atcttcagga tcgcggggtg attggggagg
    10201 gcatacgcgc cgacctggtg ctggcgcatc gcaagggcaa ccacattcat atcgaccacg
    10261 tctggcgtca gggtaaaagg gtgttctgat gacgggaaaa ctgatttggt taatgggggc
    10321 ctctggctcc ggaaaagaca gtctgttgac ggaactccgc cagcgggaac aaactcagct
    10381 actggtagcg catcgctaca tcacgcgcgc cgccagcgcc ggaagtgaaa accatatcgc
    10441 cctgagcgag caggagtttt ttacccgcgc ggggcagaac cttctggcct taagctggca
    10501 cgccaacggc ctgtattatg gcatcggcgt cgagattgac ctctggctgc acgctggatt
    10561 tgacgtggtg gtcaacggct cacgcgccca tctgccgcag gcgcgggcgc gctatcaatc
    10621 ggcgctgctg cccgtttgtt tacaggtttc gccggagatc ctgcgccagc gcctcgaaaa
    10681 ccgtggtcgt gaaaatgcca gtgaaattaa cgcccgactg gcacgcgctg cccgctatac
    10741 tcctcaggat tgtcttacgc tcaataatga cggcagcctg cgccagtcgg tcgacaagct
    10801 gctgacgctg attcatcaga aggagaaaca ccatgcctgc ttgtgagctt cgcccggcta
    10861 cgcagtacga caccgacgca gtttacgcgc tgatttgtga gctaaaacag gcggagtttg
    10921 accaccacgc gtttcgcgtg gggtttaacg ccaatctgcg cgacccgaac atgcgctacc
    10981 atttggcgct gctggatggc gaagttgtcg gcatgatcgg cctgcatttg cagtttcatc
    11041 tgcatcatgt caactggatc ggcgaaattc aggaactggt ggtgatgccg caggcgcgcg
    11101 gtctgaacat cggcagtaaa ttactggcat gggcagaaga agaagcccgc caggccgggg
    11161 ccgaaatgac cgagctttcc accaacgtga agcgccacga cgcgcaccgt ttctatctgc
    11221 gcgaaggcta cgagcagagc catttccgct tcaccaaggc gctgtaacat gagcctgacc
    11281 ctcacactta ctggcactgg cggcgcacag ggcgttcccg catggggctg tgagtgcgcg
    11341 gcctgcgcca gagcgcggcg ctcaccgcag tatcgccgcc agccatgcag cggcgtcgtg
    11401 aagtttaacg acgcgatcac cctgatcgac gccgggctgc acgatctcgc cgatcgctgg
    11461 tcgcccggat cgttccagca gtttttgctg acgcactacc atatggatca cgtgcagggg
    11521 ctgtttccgc tgcgctgggg cgtgggcgat cagatcccgg tctacggccc gccggatgaa
    11581 cagggctgcg acgatctgtt taaacatccg ggcctgcttg atttcagcca cacgatggaa
    11641 ccgtttgtgg tgtttgattt acaggggtta caggtcacgc ccctgccgct caaccactca
    11701 aaactcacct tcggctattt gctggaaacc gcgcacagcc gcgtggcgtg gctgtctgac
    11761 accgcaggcc taccggaaaa aacgctgaaa tttttacgca ataatcaacc gcaggtgatg
    11821 gtgattgatt gcagccatcc gccgcgcgag gatgcgccac gtaatcactg cgatttaaac
    11881 accgtgctgg cactgaatga ggttatccgc tcgccacggg tgattctgac gcatatcagc
    11941 caccagtttg atgcgtggct gatggaaaac gcactgccgt cggggtttga ggcggggttt
    12001 gatgggatgg agattggggt ggcgtgatgg gagggaatgt gcgcgctggc cccctcaccc
    12061 taaccctctc cccagagggg cgaggggacc gattgtgctc gatattgaat attgcgctgg
    12121 ttttctccct cgccccattg gggagagggc cggggtgagg ggcgatgcct gctccatacc
    12181 caacatcaac gcccatactc atcttccatt ctccgctctt catcttctag ctgccgacgc
    12241 tcctgatcaa gctggcgctg gcgatcgtcc agctgcctgc ggcgatcttc aaactggcgg
    12301 cggcgatcgt catattgtct gcgccgatcg tcgttcactt cacgctgcca tccgtcgtcg
    12361 cgcgaatctt catagtctcg cccacggtca gggttataag catcgttaat cgcctgctga
    12421 atattgccaa tggtgtcgtc gataatatcg gcctgggccg gaacggtgga cagcgtgagc
    12481 agggtgaata aaagaaatag cggaaagcgt ttcattagcc aacctcaaaa agaaactcta
    12541 tccacattag tcattactca tccatgcaag tagtggatga atctcaattt ctccgctgct
    12601 ctattgccgt aatcgcctcc acgcgttgtt gatgacgacc gccttcgtac tgcgcgccca
    12661 gccacgcatc cacaatcatt tttgccagtt cgaggccaac cactcgtgaa ccaaaagcca
    12721 gtgcaacttc actggcgtaa tgcggataat cagtgcgctc tgacgacctg gtttctttat
    12781 caatcccctc actcattgac catgaataaa aggctggcac agcaacgcag aatcctgcgg
    12841 tgccagcgac atgaccgccc attgctccgg tacgtttttt aataaaaaac accggttgca
    12901 ttgattaatt gtgctca
//
LOCUS       DACBVI010000127        12901 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.127, whole genome shotgun sequence.
ACCESSION   DACBVI010000127 DACBVI010000000
VERSION     DACBVI010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.127"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            155..1213
                     /gene="citC"
                     /locus_tag="IU325_17445"
     CDS             155..1213
                     /gene="citC"
                     /locus_tag="IU325_17445"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAP5810786.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1228..1524
                     /gene="citD"
                     /locus_tag="IU325_17450"
     CDS             1228..1524
                     /gene="citD"
                     /locus_tag="IU325_17450"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAP5810787.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1521..2429
                     /gene="citE"
                     /locus_tag="IU325_17455"
     CDS             1521..2429
                     /gene="citE"
                     /locus_tag="IU325_17455"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAP5810788.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2440..3972
                     /gene="citF"
                     /locus_tag="IU325_17460"
     CDS             2440..3972
                     /gene="citF"
                     /locus_tag="IU325_17460"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAP5810789.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3976..4527
                     /gene="citX"
                     /locus_tag="IU325_17465"
     CDS             3976..4527
                     /gene="citX"
                     /locus_tag="IU325_17465"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAP5810790.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4502..5380
                     /gene="citG"
                     /locus_tag="IU325_17470"
     CDS             4502..5380
                     /gene="citG"
                     /locus_tag="IU325_17470"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAP5810791.1"
                     /translation="MSMPATSTKNTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEEVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQSITPITICATAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5430..6893
                     /gene="citT"
                     /locus_tag="IU325_17475"
     CDS             5430..6893
                     /gene="citT"
                     /locus_tag="IU325_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HAP5810792.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7007..7813
                     /locus_tag="IU325_17480"
     CDS             7007..7813
                     /locus_tag="IU325_17480"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HAP5810793.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQFGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8043..8453
                     /locus_tag="IU325_17485"
     CDS             8043..8453
                     /locus_tag="IU325_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HAP5810794.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8809..9237
                     /gene="uspG"
                     /locus_tag="IU325_17490"
     CDS             8809..9237
                     /gene="uspG"
                     /locus_tag="IU325_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HAP5810795.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAK
                     ELDADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(9358..10923)
                     /gene="ahpF"
                     /locus_tag="IU325_17495"
     CDS             complement(9358..10923)
                     /gene="ahpF"
                     /locus_tag="IU325_17495"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP5810796.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVIGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(11094..11657)
                     /gene="ahpC"
                     /locus_tag="IU325_17500"
     CDS             complement(11094..11657)
                     /gene="ahpC"
                     /locus_tag="IU325_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HAP5810797.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            12029..12775
                     /gene="dsbG"
                     /locus_tag="IU325_17505"
     CDS             12029..12775
                     /gene="dsbG"
                     /locus_tag="IU325_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HAP5810798.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         3061 a         3390 c         3271 g         3179 t
ORIGIN      
        1 gcaatactcc ttaggatgta tagcgaaagg agaaaaagat atacctcgat cgcccccttt
       61 ctcccaagtg aaaataaaag gttatcagtt tgcaacatcg aacaacattt gttgcaaatc
      121 gataacaaca tgcaccttca ggatactatt tattatgttc ggcaatgata ttttcacccg
      181 tgtaaaacgt tcagaaaata aaaaaatggc ggaaatcgcc caattcctgc atgaaaatga
      241 tttgagcgtt gacaccacag tcgaagtatt tattaccgta acccgcgatg aaaagcttat
      301 cgcgtgcggt ggaattgccg gaaatattat taaatgcgtt gctatcagtg aatccgttcg
      361 cggtgaagga ctggcgctga cattagccac cgagctaatt aacctcgcct atgagcggca
      421 cagcacgcat ctgtttattt ataccaaaac cgaatacgag gcgctgttcc gccagtgcgg
      481 tttttccacg ctgaccagtg tacccggcgt gatggtgctg atggaaaaca gcgccacgcg
      541 actgaaacgc tatgccgaat cgctgaaaaa atttcgtcat ccagggaaca agattggctg
      601 cattgtgatg aacgccaatc cctttacgaa tggtcaccgt tatctgattc aacaggctgc
      661 agcacagtgc gactggttac atctgttttt agttaaagaa gattcttcac gctttcccta
      721 tgaagaccgg ctggatctgg tgttaaaagg caccgccgat attccacgcc tgactgtgca
      781 tcgcggctcc gaatacatca tctcccgcgc tacgttccct tgctacttca ttaaagaaca
      841 gagcgtcatt aaccattgtt acaccgaaat tgatctgaag attttccgtc agtacctcgc
      901 tcccgcgctg ggtgtaactc accgctttgt cggtactgaa cccttttgtc gcgttaccgc
      961 ccagtacaac caggatatgc gctactggct ggaaacgccg actatctccg caccgcccat
     1021 cgaactggtt gaaattgagc ggctgcgtta ccaggagatg ccgatatccg cttcccgggt
     1081 acgtcaactg ctggcgaaaa acgatctcac ggctatcgcg ccgctggtcc ctgcagtcac
     1141 gctgcattat ttgcagaacc tgcttgagca ctcccgccag gacgcggcag ctcgtcaaaa
     1201 gacccccgca tgagaaacag gtgaaaaatg aaaataaacc agcccgccgt tgcaggcacc
     1261 cttgagtctg gggatgtgat gatacgcatc gccccactcg atacgcagga tatcgacctg
     1321 caaatcaaca gcagcgttga gaaacagttt ggcgatgcaa ttcgcaccac cattctggac
     1381 gttctcgccc gctacaacgt gcgcggcgta cagctgaatg tcgatgacaa aggcgcactg
     1441 gactgcattt tacgtgcacg actggaagcc ctgctggcac gcgccagcgg tatcccggct
     1501 ctgccatggg aggattgcca atgatttccg cttcgctgca acaacgtaaa actcgcaccc
     1561 gccgcagcat gttgtttgtg cctggtgcca atgccgcgat ggtcagcaac tccttcatct
     1621 acccggctga tgccctgatg tttgacctcg aagactccgt ggcattacgt gaaaaagaca
     1681 ccgcccgccg catggtttac cacgcgctgc aacatccgct gtatcgcgat attgaaacca
     1741 ttgtgcgtgt aaacgcgctg gattccgaat ggggtgttaa cgacctggaa gccgtcgttc
     1801 gcggtggtgc ggacgttgtg cgtctgccga aaaccgatac cgctcaggat gttctggata
     1861 tcgaaaaaga gatcctgcgt atcgaaaaag cctgtggtcg tgaacccggc agtaccggct
     1921 tgctggcggc gattgaatct ccgctgggca ttacccgcgc agtggaaatc gctcacgctt
     1981 ccgagcgttt gatcggtatc gccctcggtg cagaagacta tgtgcgcaac ctgcgtacag
     2041 aacgctcccc ggaaggaact gaactgctgt tcgctcgctg ctccattttg caggccgcgc
     2101 gctctgcggg tattcaggcg ttcgataccg tctattccga cgctaacaac gaagccggat
     2161 tcctgcaaga agccgcccac atcaaacagc tgggctttga cggcaaatcg ctgatcaacc
     2221 cgcgtcagat tgatctgctg cacaacctct acgcaccgac ccagaaagaa gtggatcacg
     2281 cccgccgcgt cgtagaagcc gctgaagccg ccgctcgcga aggcctcggc gtggtttccc
     2341 tgaacggcaa gatggtggac ggtccggtta tcgatcgcgc ccgtctggtg ctctcccgtg
     2401 cagaactttc cggcatccgc gaagaataag gcaatcaaaa tgacgcagaa aattgaacaa
     2461 tctcaacgac aagaacgggt agcggcctgg aatcgtcgcg ctgaatgcga tcttgccgct
     2521 ttccagaact cgccaaagca aacctaccag gctgaaaaag cgcgcgatcg caaactgtgc
     2581 gccaacctgg aagaagcgat tcgtcgctct ggtttacagg acggcatgac ggtttccttc
     2641 catcacgctt tccgtggcgg tgacctgacc gtcaatatgg tgatggacgt catcgcgaag
     2701 atgggcttta aaaacctgac tctagcctcc agctccctga gtgattgcca tgcgccgctg
     2761 gtagaacaca ttcgccaggg cgtggttacc cgcatttata cctctggcct gcgcggtcca
     2821 ctggcggaag aaatctcccg cggtctgctg gcggaaccgg tgcaaatcca ctctcacggc
     2881 ggacgtgtgc atctggtaca gagcggcgaa ctgaatattg atgtggcttt cctcggcgtc
     2941 ccatcctgtg atgaattcgg taatgccaac ggctacaccg gtaaagcctg ctgcggctcc
     3001 ctcggctatg caatggttga tgccgacaac gcgaaacagg ttgtgatgct cactgaagaa
     3061 ctgctgcctt atccgcataa tccggcaagc attgagcaag atcaggttga tctgatcgtc
     3121 aaagttgatc gcgttggcga tgccgcgaaa atcggcgctg gcgcgacccg tatgaccact
     3181 aacccgcgcg aactgcttat tgcccgtagc gctgcggatg tgattgtcaa ctcaggctac
     3241 tttaaagaag gtttctccat gcagaccggc accggcggcg catcgctggc ggtaacccgt
     3301 ttcctggaag acaaaatgcg tagccgcgat attcgcgccg acttcgccct tggcggtatt
     3361 accgcaacga tggttgatct gcacgaaaaa gggctgatcc gcaaactgct ggatgtacag
     3421 agctttgaca gccacgctgc acaatcgctg gcccgtaacc ccaatcacat cgaaatcagc
     3481 gccaaccagt acgctaactg gggttcaaaa ggcgcatcgg ttgatcgtct cgacgtggtg
     3541 gtactgagcg cgctggaaat tgacacccag ttcaacgtta acgtgctgac cggctctgac
     3601 ggcgtactgc gtggtgcttc cggtggtcac tgcgataccg cgattgcctc tgcgctttcc
     3661 atcatcgtcg cgccgctggt acgcggtcgt attccgactc tggtggataa cgtgctgacc
     3721 tgcatcaccc cgggctccag tgtcgatatt ctggtcactg accacggtat cgcagttaac
     3781 ccggcacgtc cggagctggc agaacgtctg caggaagcgg gcattaaagt ggtttccatt
     3841 gagtggctgc gtgaacgtgc gcgtctgctg accggtgaac cacagccgat tgaattcacc
     3901 gaccgcgtcg ttgccgttgt gcgttaccgc gatggctcgg tgatcgatgt tgtgcatcag
     3961 gtgaaggaat aagccatgca cctgcttcct gaactcgcca gccaccatgc ggtatcaatt
     4021 cccgagctgc tcgtcagccg ggatgaaagg caagcacggc aacacgtctg gctcaagcgc
     4081 catcctgttc cactggtctc ctttaccgtg gttgcgcctg ggccgattaa agacagcgag
     4141 gtcacacgcc gaatttttaa tcatggcgtg acagccttgc gtgccttagc cgcaaaacag
     4201 ggctggcaaa ttcaggagca ggctgcactg gtttccgcca gcgggccgga gggcatgttg
     4261 agcattgccg ccccggctcg cgacctcaag ctcgccacca ttgagcttga acatagtcat
     4321 cctctcgggc ggttatggga tatcgatgtc ctgacacccg aaggcgaaat tctctcccgc
     4381 cgcgactatt cactgccgcc tcgccgctgc ctgttgtgcg agcaaagcgc agctgtttgc
     4441 gcgcgtggaa aaacccatca actgaccgat ttactcaacc gcatggaggc gctgctgaac
     4501 gatgtcgatg cctgcaacgt caactaaaaa cacaaagctt gcgacgtcat taatcgatga
     4561 gtacgccctg ctgggctggc gcgccatgct gactgaagtc aatctgtcac cgaaaccagg
     4621 cctcgtggat cgcattaact gcggtgcgca taaagatatg gcgctggaag atttccaccg
     4681 cagcgcgctg gcgattcagg gctggttacc ccgtttcatt gaatttggtg cctgtagtgc
     4741 tgaaatggca ccagaagagg tactccacgg attacgccca ataggtatgg cttgcgaagg
     4801 tgatatgttc cgcgccactg cgggcgtaaa cacgcataaa ggcagcattt tttctttagg
     4861 gctactgtgt gcggcaattg gccgtttgct tcaactcaac cagtcgataa cgccaataac
     4921 aatttgtgcg acggcggcaa gtttctgccg tggcctgacc gatcgcgaac tgcgtaccaa
     4981 taattcacaa ctgacggcag gtcaacggtt gtaccagcag cttggcctta ccggcgcacg
     5041 cggtgaagcc gaagcgggtt atccactggt gatcaaccac gccctgccgc attacctcac
     5101 tctgctggat caggggttag atcctgaact ggcactgctc gataccttac tcctactgat
     5161 ggcgatcaac ggcgatacca acgttgcatc gcgcggtggc gaggggggcc tgcgctggct
     5221 acagcgcgag gcgcaaacat tattgcaaaa agggggcatc cgaacccccg ccgatctcga
     5281 ttatctccgg cagttcgaca gggagtgtat cgaacgaaat ctcagtccag gcggcagtgc
     5341 cgacctgctg atccttactt ggtttttagc acagatttaa ttatttaagc acttgataaa
     5401 attggaatat taattgtcgg agaacccgta tgtctttagc aaaagataat atatggaaat
     5461 tattggcgcc cctggtggtg atgggtgtca tgtttcttat ccctgtacct gacggtatgc
     5521 cgccgcaggc gtggcattac tttgcagtgt ttgtggcaat gattgtcggc atgatcctcg
     5581 agccaattcc ggcaacggcg atcagtttta ttgcggttac tatttgcgtt attggcagta
     5641 attacctgct ctttgatgcc aaagaattag ctgacccagc gtttaatgcg caaaaacagg
     5701 cgctgaaatg gggtctggct ggtttttcca gcaccacggt atggctggta tttggcgcat
     5761 ttatttttgc attagggtat gaagtttccg ggttaggtcg tcgcattgcc cttttcctgg
     5821 tgaaattcat gggcaaacgc acgctgacgt tgggttatgc gattgtcatt atcgacattc
     5881 tgctggcacc gtttacaccg tccaacaccg cgcgtaccgg gggtacggtt tttccggtca
     5941 ttaaaaacct gccgccgctg tttaaatcat tcccgaacga tccgtccgcg cgtcgtattg
     6001 gcggctattt gatgtggatg atggtcatta gtaccagtct gagttcgtcc atgtttgtca
     6061 ccggtgcggc accaaacgta ctgggtctgg agttcgtcag caaaattgcc ggtatccaga
     6121 ttagctggtt gcagtggttc ctctgcttcc tgccggttgg ggttatcttg cttatcattg
     6181 cgccgtggct ttcctacgtg ctgtacaaac cggaaatcac acacagtgaa gaagtggcaa
     6241 cctgggcggg tgatgaacta aaaaccatgg gtgcgctgac acgcagagag tggacgctga
     6301 ttggtcttgt attgctcagc ttaggtttgt gggtatttgg cagtgaagtc attaatgcta
     6361 ctgcggtagg tctgctggca gtttcgctaa tgctggccct gcacgttgtg ccgtggaaag
     6421 acattacccg ctataacagc gcatggaaca cactggtcaa cctggcaact ctggttgtga
     6481 tggctaatgg cctgactcgt tctggtttta ttgactggtt cgccggtacc atgagtacgc
     6541 acctggaagg attctcacca aacgcaacgg tgattgtact ggttctggtg ttctactttg
     6601 cgcactacct gtttgccagc ctgtctgcgc acaccgcaac catgctgccg gttattctgg
     6661 ccgtcggtaa aggtattccg ggcgtaccaa tggaacaact gtgtatcctg ctggtgctgt
     6721 ctatcggtat catgggctgt ctgacgccgt atgcaaccgg tcctggggtg attatttacg
     6781 gctgtggcta tgtgaaatca aaagattact ggcgtcttgg cgcaatcttc ggggtgattt
     6841 acatctctat gttgctgttg gttggctggc cgattctcgc catgtggaac taatacgttc
     6901 gccaaacgct gaataaatca tcacgcccgc caggtaagcc cacctggcgg gcttttttat
     6961 gatttaatag atagtattca gtgtatgtat gagttccaca cccattatga aagcattctg
     7021 gcgtaacgcc gcgttgctcg cggtttctct gcttcccttc tcttctgcca acgccgtagc
     7081 gttgcaggca aaacagtttg gcgattttga tcgctatgtc ctggccctct cctggcaaac
     7141 cggattttgc cagagtcaac acgatcgaaa tcgtaacgaa cgagatgaat gtcgcctgca
     7201 aaccgaaacg accaacaaag ctgattttct gaccgtacat ggtctgtggc caggattgcc
     7261 taaatcggtt gctgcccgtg gtgttgatga acgccgctgg atgcgcttcg gttgcgctac
     7321 tcgcccaatc ccgaatctac cagaagcgca cgccagccga atgtgttcat cgccggaaac
     7381 cgggttatcg ctggaaacag ccgctaaact aagtgaagtc atgccaggag ctggcggacg
     7441 ttcctgcctg gaacgctacg aatatgccaa acacggtgcc tgctttggtt ttgatccaga
     7501 cgcatacttc ggcacgatgg tacgcctgaa tcaagaaatt aaagagagcg aagccggaaa
     7561 attccttgcg gataactacg gtaagacagt gagccgccgt gactttgacg ccgcctttgc
     7621 caaaagctgg ggaaaagaga acgtgaaagc agttaagcta acgtgtcagg gtaaccctgc
     7681 gtatttgact gaaattcaga tctcgatcaa agctgacgcc atcaacgctc cgctttctgc
     7741 aaactcattc ttgccacaac cacacccagg caactgtggc aaaacctttg tgattgataa
     7801 agcaggttat taatcctctg atttacaaaa gacgctaatt cgtcttccat aaaccatgac
     7861 gattaagatt tatacataat gcgctacatt gaggaattcc ctgaatgtga cgcaaatcac
     7921 ttcaaagagt gagcaacagt cagtatcatc atgtgagtta aaccctcgcc gcctgacggt
     7981 gagggttttc ttttgggtat gtttatctgc gacactcgca gtaccgacga catggagtaa
     8041 aaatgtccag accaactatc atcattaacg acctggatgc cgaacgcatc gatattctgc
     8101 tggagcaacc cgcctatgct ggtttgccaa tcgccgacgc gttaaacgca gagttggatc
     8161 gcgcccaaat gtgttcgcca gaagagatgc cacacgacgt ggtgacaatg aacagccggg
     8221 ttaaattccg caatcttagc gatggcgaag tgcgtgtgcg cacgctggtg tatccggcaa
     8281 aaatgaccga tagcaatact cagctttccg ttatggctcc ggtaggtgcc gcactgctgg
     8341 ggctgcgcgt tggcgattcc attcactggg aacttccggg cggcgttgca acccaccttg
     8401 aagtgctgga actcgaatac cagccagaag ctgctggcga ctacctgctt taatcccatc
     8461 tgcacaagcg ataactcgct ttgcccagag gatgcactcg catcctcttt ccgctcaaat
     8521 atcaaccaga tttatcattt tgctcttaag cgcagcattc taatctgaac tggttattca
     8581 tctgcggaac ctcatatgag cgaagctctc ggtatcactg cattttatat attcatagcc
     8641 gcaattattg ttgctgcagt gctatacctt gaacaacgct ggtagcgcct ggttttattt
     8701 attttccagc tcaatagcaa attcagatca aatcagcctc gcaaattata gattcataac
     8761 caataatgtg ctgttatgta tacatgcaaa aacaaggaga gaatgcttat gtacaaaaca
     8821 atcatcatgc cagtggatgt tttcgaaatg gaattgagcg acaaagcggt tcgccacgct
     8881 gaattcctcg cccaggatga cggagttatt catctacttc acgtactacc ggggtcagcc
     8941 agcctgagcc tgcaccgttt tgccgctgat gtgcgtcgtt ttgaagagca tctgcaacat
     9001 gaagcagaag aacgtctgca aacgatggtc agccacttca ccatcgatcc ttcccgcatt
     9061 aagcaacatg tccgttttgg tagtgtgcgg gatgaagtta acgagttagc gaaagaactt
     9121 gatgcagatg tagtagtgat tggttcgcgc aacccatcga tttcgaccca tctgttaggt
     9181 tctaacgcct caagcgttat ccgccacgcc aatctgccgg tgctggttgt gcgttaattg
     9241 ccagaataaa tatcccgccc tgcccggagc catcccggcg gggcttttta ttgccataaa
     9301 aaagccgcca ggtttgaccc cggcggctaa gcaattgcag gtgaatctta cttcttctta
     9361 tgcagttttg gtgcgaatca ggtagtcaaa agcactcaga gaggctttgg caccttcgcc
     9421 agtggcgatg atgatctgct tgtacggaac cgtcgtacag tcacccgctg cgaacacgcc
     9481 tttcacgttg gtttcgcatt tcgcatcaat gataatctcg cccatgcggt tacgttcgac
     9541 tgcgccttcg agccagttgg tgttcggcag cagaccaatc tgcacgaaaa taccggccag
     9601 ttcgatgttg tgaatatcgc cgctgacacg atctcgatat tccagaccaa tgactttgct
     9661 gccgtcgcct ttcacttccg tagtttgcgc attcagaata atgtcgacgt ttttcaggct
     9721 gcgcagtttg tcctgcagaa cctggtcggc tttcatttct ggcgcaaatt ccagcagcgt
     9781 tacgtgctca acgatacccg ccaggtcgat tgccgcttcc acgccggagt taccgccgcc
     9841 gataaccgct acgcgtttgc ctttaaacag cgggccgtcg cagtgcgggc agtaggtcac
     9901 gcctttggtg cgatactgat cttcgcccgg aacgttcatg ttgcgccatt ttgcaccggt
     9961 cgccacgata atgctgcgtg ctttcagtac cgcgccagaa gctgtttcaa tctgatgcag
    10021 accaccttca accgctgccg ggatcagttt gctggcgctc tggctgtcga tcacatcaac
    10081 gtcgtattca tcaacgtgaa ctttcagcgc tcctgccagc ttctgccctt cagtcttcgg
    10141 tacagaaatg tagttttcga tatcaacggt atcgaggatc tgaccaccaa aacgttcgcc
    10201 catcagacca gtacggatgc ctttacgtgc ggagtaaatt gctgccgctg cacccgctgg
    10261 gccggaaccg acgattaata cgtcataagc atcacgcttg ttcagctctt ctgccgcacg
    10321 tttttccgcg ccagtatcaa ttttggcgac gatttcagtc aacgtcatgc gaccctgacc
    10381 aaactctttc ccgtttacga atactgccgg aacgcccatc acgttgcgat cggtgatttc
    10441 gttctggaag gtacctccgt caattgcagt gtgcttgatg cgcgggttca gtacgctcat
    10501 caggttcagc gcctgcacca catccgggca gttgtggcaa gagagcgagt aataggtttc
    10561 gaattcaaaa tcaccgtcaa tatggcgaat ctgctccagc agagactgcg cttctttcga
    10621 cggatgacca ccggtccaca acaacgccag taccagcgag gtaaactcgt ggcccagcgg
    10681 ggaacctgca aaacgtggcc cctggttaga acctgggttg gtgatcagga aagacggctt
    10741 acgcaccggc aagctgttat cttctttaaa ggtgactttg tctgacagtt ctgcgatttc
    10801 agccaacagt tccttgattt ctgccgattt agcgctgtca tccagcgtgg caattaactc
    10861 aacaggcttg gtcaatttct caaggtaagc cttgagttga gttttcatat ttgtgtcgag
    10921 catgtttatc tcctgggctt taaacatcat catgcaagcg gcctctgcgg ctacctggat
    10981 gcagcttgca tcatggtgcg gggaagaaaa tcgggtgccg cagcacccga agaattaggt
    11041 gaatttccga agtatttcgc gccggagttg gtagcgcgaa agacgaagga aatttagatt
    11101 ttaccaacca ggtccagaga cggagccaga gttgcttcac cttctttcca tttagccggg
    11161 caaacttcac ctgggtgaga agctacgtac tgtgctgctt tgattttacg cagcaggtca
    11221 gacgcgtcac ggccaatgcc ttcagcggta acttcgattg cctggatgat accctgcggg
    11281 tcaacaacga aggtcgcacg gtcagccaga ccttcatctt cacgcatgtt gtcgaagtta
    11341 cgggtcaggg cgccagtcgg gtcgccgatc atcgcatatt tgattttagc gatggtttca
    11401 gagctgctgt gccatgcttt gtgggtgaag tgagtatcgg tagatactgc gtatacgtct
    11461 acgcccagtt tctgcagttc ttcgtagtgg tcagcaacgt cacccagttc ggtcgggcat
    11521 acgaaagtaa agtcagccgg gtagaagaag aagacgctcc agcggccttc ggtatctttt
    11581 tcggtgattt cgatgaattc gccgtttttg aatgcctggt ttttaaaagg tttaattttg
    11641 gtgttaatca aggacatcta tacttcctcc gtgttttcga tgagatgtaa ggtaaccaat
    11701 ttttgccgat tcggctaatg cgtttccatt atcaaatcga taagttttac cttacaacct
    11761 tcgtaagaca actttgtgaa ctttgcaccg ccagcggcga taaaaagtaa aatggccccc
    11821 tttccaggtg accgtgtaat ctgaactacc ctttggtatc ttcagcgcct gcctgcaggc
    11881 tgaggtgttg cccttacaaa tgcaacaacg acatggatta caacaccctc ataaacaaag
    11941 ggcaatcacc tgatctaagc tcttacctat ggcagtgata ggttatgcct tttacccgac
    12001 ttttgcactg actgaaaagg acaaattaat gttaaaaaag atacttttac tggctctgct
    12061 tcctgcaatc gccttcgcag aggaacttcc tgccccagta aaagcgattg aaaaacaggg
    12121 cattacaatc atcaaaacgt tcgatgctcc cggaggaatg aaaggttatc tcggaaagta
    12181 tcaggatatg ggcgtcacca tctacctgac tccagatggt aagcacgcta tctccggtta
    12241 catgtacaac gagaaaggtg aaaacctgag taacacactt atcgaaaaag aaatttacgc
    12301 accagccgga cgcgaaatgt ggcaacggat ggaacaatcc cactggctcc tcgacggtaa
    12361 aaaagacgcg ccggtcattg tttacgtctt cgccgatccg ttctgcccat attgtaaaca
    12421 gttctggcag caggcgcgcc cgtgggtaga ttctggcaaa gtgcaattaa gaacattgtt
    12481 ggttggggtt atcaagccag aaagcccggc gacagcagcg gcaattcttg cctccaaaga
    12541 tcccgcaaaa acctggcaag aatatgaagc ctctggtggc aagcttaagc taaacgtgcc
    12601 tgcaaacgta agtacagagc aaatgaaagt gttaagcgac aacgagaaac tgatggacga
    12661 tctgggggca aatgtcacac cagctatcta ttacatgagt aaggaaaata ctctgcaaca
    12721 ggccgtgggg ttgcccgatc agaaaacgct taatatcatt atggggaata aataaaatat
    12781 aattcatgga tggttgaaat atatccctgc ttttcaacca tcctaaaaaa gcatgaattg
    12841 ctcgctctac cctaagtggc tgtgctattt tagaaaaact taatcctgag ttaagcaaaa
    12901 a
//
LOCUS       DACBVI010000128        12735 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.128, whole genome shotgun sequence.
ACCESSION   DACBVI010000128 DACBVI010000000
VERSION     DACBVI010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.128"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            300..674
                     /gene="tomB"
                     /locus_tag="IU325_17510"
     CDS             300..674
                     /gene="tomB"
                     /locus_tag="IU325_17510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HAP5810799.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            700..918
                     /gene="hha"
                     /locus_tag="IU325_17515"
     CDS             700..918
                     /gene="hha"
                     /locus_tag="IU325_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAP5810800.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            1090..1641
                     /locus_tag="IU325_17520"
     CDS             1090..1641
                     /locus_tag="IU325_17520"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HAP5810801.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            1757..2227
                     /locus_tag="IU325_17525"
     CDS             1757..2227
                     /locus_tag="IU325_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810802.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            2391..3941
                     /gene="pdeB"
                     /locus_tag="IU325_17530"
     CDS             2391..3941
                     /gene="pdeB"
                     /locus_tag="IU325_17530"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HAP5810803.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELNTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(3983..4336)
                     /locus_tag="IU325_17535"
     CDS             complement(3983..4336)
                     /locus_tag="IU325_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HAP5810804.1"
                     /translation="MKYVDGFVVAVPVDKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(4456..4552)
                     /gene="ffs"
                     /locus_tag="IU325_17540"
     ncRNA           complement(4456..4552)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IU325_17540"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4715..5026
                     /locus_tag="IU325_17545"
     CDS             4715..5026
                     /locus_tag="IU325_17545"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP5810805.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            complement(5057..5629)
                     /locus_tag="IU325_17550"
     CDS             complement(5057..5629)
                     /locus_tag="IU325_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810806.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            5847..6707
                     /gene="tesB"
                     /locus_tag="IU325_17555"
     CDS             5847..6707
                     /gene="tesB"
                     /locus_tag="IU325_17555"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HAP5810807.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(6756..8042)
                     /gene="amtB"
                     /locus_tag="IU325_17560"
     CDS             complement(6756..8042)
                     /gene="amtB"
                     /locus_tag="IU325_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HAP5810808.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(8072..8410)
                     /gene="glnK"
                     /locus_tag="IU325_17565"
     CDS             complement(8072..8410)
                     /gene="glnK"
                     /locus_tag="IU325_17565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP5810809.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADESAL"
     gene            complement(8591..10372)
                     /locus_tag="IU325_17570"
     CDS             complement(8591..10372)
                     /locus_tag="IU325_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAP5810810.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVSM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQSRFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(10365..12137)
                     /locus_tag="IU325_17575"
     CDS             complement(10365..12137)
                     /locus_tag="IU325_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAP5810811.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAEDAEDTGKKNMRVARIDARFDPTIYIAIGTANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIRQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            complement(12167..12625)
                     /locus_tag="IU325_17580"
     CDS             complement(12167..12625)
                     /locus_tag="IU325_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HAP5810812.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
BASE COUNT         3217 a         3424 c         3062 g         3032 t
ORIGIN      
        1 attacgtcat attcagagca atcctggtat tagtgtcacc aatttcatct ggcgataatc
       61 ctgaaatgtt atgaatagtt cgagcagact gcttttacct actgcgggtt agtgctagta
      121 tgaaaaaatg agtcctgtcc cgattccttc ctaattgtaa tttttcgtaa taatgcgatg
      181 aaaacctgca aagagtggct tatagttaag ctaacaaacg agagggcaag tccaggtcag
      241 taagtttttt ccatcccgaa aggtgtccgt tagttcaacc actaagaagg ggacgcgtta
      301 tggatgaata ctcacccaaa agacatgata tcgcacagct taagtttctc tgtgaaaccc
      361 tgtatcatga ctgtcttgca aaccttgaag aaagcaatca tggctgggtt aacgacccaa
      421 cctcggcgat caacctccag ttgaatgaac tgattgagca tattgcgacc ttcgcactta
      481 attacaaaat taagtataat gaagacaata agctcattga gcagatcgac gaatatctgg
      541 atgacacctt tatgttgttc agcagttatg gtattaatat gcaggatctt cagaaatggc
      601 ggaagtcagg taatcgacta ttccgttgtt ttgtcaatgc gacgaaagag aatcctgcga
      661 gtttatcttg ttagaattat tacaaccatg ggtagaagta tgtccgaaaa acctttaaca
      721 aaaaccgatt atttaatgcg tttacgtcgt tgccagacaa ttgacacgct ggagcgtgtt
      781 atcgagaaaa ataaatacga attatcagat aatgaactgg cggtatttta ctcagccgca
      841 gatcaccgcc tcgccgaatt gaccatgaat aaactgtacg acaagatccc ttcctcagta
      901 tggaaattta ttcgctaata aataattcgc tttcggagct ataaccggct gtttattaag
      961 aattttatac tttttcgcca tgaagacgta ccctatgtga tctttatcac acagatgtaa
     1021 tgggaacgtt ctcttcactg acttttcgtc ttactgtgtt gccgcatttt cagcaaccgg
     1081 aggtcagtaa tgagcacaga aaaagaaaag atgattgctg gtgagttgta tcgctcggca
     1141 gatgagacgt tatctcgcga tcgcctgcgc gctcgtcagc ttattcaccg atacaatcat
     1201 tccctggcgg aagagcacac attacgccag caaattctcg ctgatttatt cggtcaggtg
     1261 acagaggctt atattgagcc aacgtttcgc tgtgattatg gctataacat ttttctcggc
     1321 aataattttt tcgccaactt cgattgcgtg atgcttgatg tctgccctat tcgcatcggt
     1381 gataactgta tgttggcacc aggcgttcat atctacacgg caacacatcc catcgaccct
     1441 gtagcacgta atagcggtgc tgaactgggg aaacccgtca ccatcggtaa taacgtctgg
     1501 attggcggac gcgcggtcat taaccctggt gtgaccattg gtgataacgt cgtggtagcc
     1561 tcaggtgcag ttgtcacaaa agatgtcccg gacaacgttg tcgtgggcgg taatccagcc
     1621 agaataatta aaaaattgta ataggttttt cgcaactgtt atgcaaaatt gtggtaaatc
     1681 tgttactttc cctctactat tcccacgtta aaatagggtg ttccctggaa agttgcagat
     1741 accacgaagg caaacgatga ccgaaataca acgcctgctg accgaaacaa ttgagtctct
     1801 aaatacccgc gaaaaacgcg acaacaaacc ccgctttagt atcagtttta tccgtaaaca
     1861 tccggggctg tttatcggta tgtatgttgc tttttttgcc accctggcgg tgatgttgca
     1921 gtccgaaacg ctttcaggct ctgtctggct actggttgta ttatttatcc tgcttaatgg
     1981 tttcttcttt ttcgatgtct acccacgcta ccgctatgaa gatatcgacg tgctggattt
     2041 ccgcgtttgc tataacggcg aatggtacaa cacgcgcttt gtacctgccg cgctggttga
     2101 agccatcttg aactctccgc gtgtcgcgga tgttcataaa gaacaactgc aaaaaatgat
     2161 cgtccgtaaa ggtgaactgt ctttttacga tatttttacc ctcgctcgcg ccgaatcaac
     2221 atcttaagtt agggttacat accaggcgta aagctctgcg cctggtcaaa tgacaacgtt
     2281 cgtttccacc catcccttca tgaaatcccg gctctacctc cttatctcct gcaagccttt
     2341 ttccacaatc agatatactt tccctacact gtgttaataa ggatatggtg gtgagaacac
     2401 gacatctggt cggccttatt tcgggagtac tgattctttc agtattgctg cctgtcggct
     2461 taagcatctg gctggcccat cagcaggtag aaacatcatt tattgaagag ctgaatacgt
     2521 attcctcccg cgtcgctatt cgggccaata aggtggcgac acaagggaaa gatgcgctgc
     2581 aagagctgga aagatggcaa ggcgctgcct gtagcgaagc ccatctcatg gaaatgcgtc
     2641 gggtatctta cagttatcgc tatattcagg aagtggttta tatcgataac aacgttcccc
     2701 agtgttcgtc tctggagcat gaaagcccgc ccgatacctt ccccgagcca ggtaaaattt
     2761 cgaaagatgg ttatcgtgtc tggttaacat cgcataacga tttaggcatt atccgttaca
     2821 tggttgccat gggaacggca cattatgtcg tcatgatcga ccccgcttcc tttattgatg
     2881 tcattcccta tagctcatgg caaattgatg ccgccattat tggcaatgcc cataacgttg
     2941 tgataaccag cagcgatgaa attgctcagg gaattattac cagactacaa aaaacacccg
     3001 gtgagcatat cgaaaataat ggaatcattt acgatatcct gcccttcccg gaaatgaata
     3061 tttcgatcat cacctgggct tcaacgaaaa tgttgcagaa aggctggcat cggcaagtct
     3121 ttatttggtt accgctcggg ttggtgattg gcctgctggc agcgatgttt gtgctgcgta
     3181 ttttgcgccg tatccagtca ccgcatcatc ggctgcagga tgctatcgaa aatcgtgata
     3241 tttgcgtgca ctatcagccg attgtctcct tagccaatgg caaaattgtc ggtgctgagg
     3301 cactggcgcg ctggccgcag acagacggta gttggttgtc accagatagt tttattccgc
     3361 tggcacagca aacgggcctt tctgagccat tgacgctact gattataaga agcgtctttg
     3421 aagatatggg cgactggctg cgtcagcatc cgcagcagca tatttcgatc aatcttgaat
     3481 ccaccgtgct cacctcggaa aaaatcccgc aattgctgcg tgaaatgatc aatcactatc
     3541 aggttaatcc cagacagatc gcgcttgaac tcactgaacg cgagtttgcc gatccgaaaa
     3601 ccagcgcccc gataatttct cgctaccggg aggcgggcca tgaaatttat cttgatgatt
     3661 ttggtacggg gtattcaagt ttaagttatt tacaggatct ggatgtcgac attctgaaga
     3721 tcgataaatc tttcgttgat gcgctggaat ataaaaatgt cacgccgcat atcatcgaaa
     3781 tggcaaaaac actgaaactg aaaatggtag cggagggaat cgaaaccagt aaacaagaag
     3841 agtggttacg ccagcatggc gtgcactacg gtcagggctg gctctacagc aaggcattac
     3901 cgaaagaaga tttcttacgc tgggccgagc aacatttgtg aattgtaggg ctgacggcac
     3961 gccgcacagc ccatcacgct acctattcgt cgatgattga ctcgaatccg ccatagatca
     4021 ttcgcttacc atcaaacggc atggactcgc cgaactcttt catccgtggg tccgacatca
     4081 tcttttgatt agcagcgtcg cggacctctt ttgaagggta ttcaatccag ctaaagacaa
     4141 cctcttcatt ctcttcagct tttaccgcca tacgaaaatc ggtcactttg ccatccggta
     4201 catcgctggc ccagcattcg acaatacgaa gcgcgccaaa ctctttaaac aatggcgcag
     4261 ccttagcagc catttcccga taggcatcct ttttatcgac aggaacggca accacaaaac
     4321 catcaacata cttcatcaga catacctccg aagcgtactg cgcagccaga caattcttgc
     4381 gacgtcagtt gtggttaaag tttagccata ttgggatttt ttgacgaaac ggggggaggc
     4441 agaaatgggt gggggccctg ccagctacat cccggcacac gcgtcatctg ccttggctgc
     4501 ttccttccgg acctgacctg gtaaacagag tagcgttgcg ggagaaccaa cagagccccc
     4561 attgagagcg ttgagaacca acgcgcaggc gcattatcac tgctgccccg gctaattgca
     4621 acccattgcg tgccggatgc tggtttcttg cgcaatgcgg cttcactcgg gcgtttttcc
     4681 agattatgct gaaaaattac ctcaggaaga aaagatggaa aaagaagatt catttcccca
     4741 acgcgtctgg caaatcgtcg ccgctattcc cgaaggctat gtcaccactt acggtgatgt
     4801 ggcgaaactg gcaggatcgc cccgcgccgc gcgccaggtg ggcggtgtgt taaagcgtct
     4861 ccccgaaggc agcaccttac cctggcaccg ggtggttaat cgccacggca caatttcgct
     4921 aaccggaccg gatttacagc gtcaacgaca ggcattactg gcagaaggtg tgatggtatc
     4981 gggaagcggg caaatcgact tgcagcgtta tcgctggaac tactaaccct ctccgggcgg
     5041 agagggttaa ctgcgcttaa tattgcgtag gagctggaac tgccgaagac ggattcacct
     5101 gagttggtga agtcgaaggt acggtagtcg ttgcgccacc gctggcctga accggcacgg
     5161 cggtttgctg caccggcacc aatgtcaggt cggctttagt tccgccctgg ttgatcaccg
     5221 gctgaacggt atcggtaata aataccagtt tgtcattcac ggtaatcgcc gcactcaaca
     5281 gaatacgcgc attcggctga acatctgccg ggttaaatgg cagaacaaag ctgaatggtg
     5341 actgtttgcc ttcggtacgc accgctttct gcgccagtac ttttgacggt gcatcggcta
     5401 acgacgcgtc agaaagtgtc acggtcagca cagcatcagg cggcagtgcg actttctgac
     5461 ggatccagac ggtaccggag acattcggtt gctggatagc aggttgttgt gttgctgaaa
     5521 tagacgtatt tgcagccggt gctggcgtct gaatatccgc gcttttatct gcgcaagccg
     5581 ccaacgcaat cgcaaccgct aaaccactgg ccatgtgcac gagtttcatt cacttctcct
     5641 tattatcaat gcaccagcgg gctaactttc ctcgccggaa gagtggttaa caaaatagta
     5701 acgtcaacaa gtgtggcaca catcacgcat ttctgcctgt aattagcccg taattcagac
     5761 cattgcaccc atcggaccag tggcaaattt gcgttatact caactcactt tggcttgctg
     5821 cggcagcttt gttactggag agttatatga gtcaggcgct aaaaaattta ctgacattgt
     5881 taaatctgga aaaaattgag gaaggactct ttcgcggcca gagtgaagat ttaggtttac
     5941 gccaggtgtt tggcggccag gtcgtgggtc aggccttgta tgctgcaaaa gagaccgtcc
     6001 ctgaagagcg gctggtacat tcgtttcata gctactttct tcgccctggc gatagtaaga
     6061 agccgattat ttatgatgtc gaaacgctgc gtgacggtaa cagcttcagc gcccgccggg
     6121 ttgctgctat tcaaaacggc aaaccgattt tttatatgac tgcctctttc caggcaccag
     6181 aagcgggttt cgaacatcaa aaaacaatgc cgtccgcgcc agcgcctgat ggcctgcctt
     6241 cggaaacgca aatcgcccaa tcgctggcgc acctgctgcc gccagtgctg aaagataaat
     6301 tcatctgcga tcgtccgctt gaagtccgtc cggtggagtt tcataaccca ctgaaagggc
     6361 acgtcgcaga accgcatcgt caggtgtgga tccgcgcaaa tggtagcgtg ccggatgatc
     6421 tgcgcgttca tcagtatctg ctcggttacg cttctgatct taacttcctg ccggtagctc
     6481 tacagccgca cggcatcggt tttctcgaac caggaattca gatagcgacc atcgaccatt
     6541 ccatgtggtt ccatcgcccg tttaatttga atgaatggct gctgtatagc gtggagagca
     6601 cctcggcgtc cagcgcacgc ggctttgtgc gcggtgagtt ttatacccag gacggcgtgc
     6661 tggttgcctc gaccgttcag gaaggagtaa tgcgtaatca caattaaaaa aatagccgga
     6721 ggtgaaaacc gtccggctgt tttttgcagt gcttgttacg cgttataggc attttcgccg
     6781 tggctgttga cgtcaagccc ttctcgctcc tgttcttccg gcacacgcag gccaaccgtc
     6841 agatccgcca gtttgtagcc gataaatgcc accacaccgg accagacgat cgtaatggcg
     6901 atgctttcca gctgtaccag caactgatgg cccatcgtca caccttcagc gaagcccacg
     6961 ccgcccagcg aactggcggc aaaaatcccg gtcatgatac agccgacaat gccacaaacg
     7021 ccgtgcacac cgaagacatc gcagggatca tccacccgca gcaagcgttt gagcatggta
     7081 acgccccaca agcccgccag accagctacc acgccgataa tcaacgcgcc gccaacacca
     7141 atgtagccac acgctggcgt cacgccgacc agaccggcaa tcgcgccaga acacgctccc
     7201 agcagtgaag gctttccgcg cagcgcccat tcaccgaaga tccagccaag aattgctgcc
     7261 gccgttgcga ccacagtatt cacaaatgcc agtgccgcga tttcattcgc cgtgcccgct
     7321 gacccggcgt taaagccaaa ccaaccgata tagagaatgg cagtcccggt gaagaccatc
     7381 ggcaggttgt gcggtttaaa cgcctcttta ccgaagccca cgcgtttgcc tatcagatat
     7441 gcgcccacca gaccggcgat tgcggcgtta atgtgcacca ccgtgccacc cgcgaaatcc
     7501 agcgcaccgt gagaagccag caaaccaccg ccccagacca tatgcgcaat cggaatgtag
     7561 gagagcgtca gccataccac cacgaaaatc aacacagctg agaagcggat tcgttccgcc
     7621 agcgccccaa ctatcaagcc tacggtaatg caggcaaacg atccctgaaa cgctacgtgg
     7681 atatactgat aaatgctgcc catcaccgcc gtcagttcga tgtttttcag catcaaccag
     7741 ttaatgttgc cgaagaagtt gtttccctca ccaaacgcca gcgagtaacc gtaaaccacc
     7801 cagagaatac agaccagtgc aaacgtcacc gtcacctgcg tcagcatcga cagcacgttt
     7861 ttgccgcgaa tcaacccacc gtaaaacagg gcaatccccg gaatagtcat aaacagcacc
     7921 agcgcagtac aaatcatcat aaacgcattg tcggctttat cggccaccgc aggtgcagcc
     7981 attaccagtc ccggcagcat cgccagtgaa gcaagcccag tttttatcgt cgctatcttc
     8041 atttttcgtt cctgttgctg tgtgccagag attacagcgc cgattcgtcg gcttcgccgg
     8101 tacgaatacg aatgacgcgt tgcaattcag cgacgaagat tttgccgtcg ccaatttttc
     8161 cggtgtaagc cgctttgctg acgatatcga tcacttcatc gagttggtca tcagcaatcg
     8221 ccacatcaat ttttactttt ggcaggaaat tgacgctgta ttccgccccc cggtacagct
     8281 cggcatgtcc tttctgacgc ccgaaacctt tcacttcggt gacggtcagg ccctgaatac
     8341 caatggaaga taacgcttca cgaacgtctt ccagcttgaa tggtttgatt atcacggtca
     8401 ccagcttcat agatcccctc cggtcagaat tcggtaatgg ttctgctaca cgaagaaggt
     8461 attgcaagcg gtgtgccaga aatgaaaaac gagaaggaga gcaggaagtt aagaagattg
     8521 aaaacaaaaa cgcactatga cagtgcacag tgcgttacat ttttgcacca acgatgaaat
     8581 attgcgctat tcaggcgctc aatgattcct cttcacgcac gctggctgcc agctcttcgc
     8641 ccgcaagttg cagttgatac atctgccagt agcgtccctg ggccgccagc agttgctggt
     8701 gagtaccctg ctccacggct tgcccacgat gaagcaccag aatggtgtcg gcatcaacaa
     8761 tggtcgataa gcggtgagca atcaccacca gcgtggtatg ttcacgcacc gccgccagag
     8821 catgttgaat cgcctgttca gtaccggagt caatgctggc ggttgcctca tcaaggatca
     8881 ggatttgcgg cgtctccacc aacacgcgtg ccagtgccag cagttgcttt tgcccgactg
     8941 agagattatt cccctgctcg ccaagcggcg tgtaaatacc gtcgctcatg ctacgcgcca
     9001 gctccgccag ttgcacggtt tccagcgcct gccagacgcg ttcttcggag atatcacgcc
     9061 ccagcgtcac gttggcgagg aaggtatccg ccagcaccac cggatcttgc tgcaccattg
     9121 ccacgccctg gcgcagcgcg ctgtgactta gcgaacttaa tggacgacca tcaaggcgaa
     9181 tctcaccttc cgttagcggg taatagccca tcaataaact ggcgagggtg cttttgccac
     9241 tgccggtatg cccgaccagt gccacaaagt tacgcgaagg cacagagaga ttaatgttct
     9301 ttagcaccag attgtcatcg cgataagcaa atgacacgtt atcgacttcg atggtgccgc
     9361 tctgtaacgg gcgatcatca ttgccatatt gctggcgcgg tccgtccatc agttcaaaca
     9421 cacgctcacc agcaactaca gcctgttgca gcatcgcctg ctgcgtggtc agttcgatta
     9481 atggttcgtt aagtcgccca agatagctga taaatgcata caacacgccc acttcaatgg
     9541 tgccgctggc ggagaagcca aacagcatca acaacccaca aagaatgagc gatgaaaaca
     9601 gactcagcag cggacgcagc agaaaaccgt cgaggcgcag ggtttgcatc ctcgccatat
     9661 agtgtgaacg actggcctca cccatacgtt cgccaaatcg cgactgctga cggaactgct
     9721 ggataacgct catgccattg atgatttcgt taaagccgtc gttgatatct gccagataag
     9781 cgcgtacacg gcggacgatc ggcgtgctgt aacgctgata tatcaccatt accaccagca
     9841 ccaccgggaa aatcattatc gccaccagcg ccattcgcca gtcgaggctg aacatcgaca
     9901 ccagcatcgc gcccaccagt gcggcgctac gcaggacagt tgccactacg gtaacgtaga
     9961 ggtcgcggat cacttcagtg tcattagtga cgcgggaaat cacctgcccg acgggttggg
    10021 tatcaaactc gcttaatggc tggcgtaacg cagcatccat cacgtcggta cgcaactgtt
    10081 gcactacgcc tactgccgcc cgattaaaca gcagcgactg cgcgtaatgt agcccggcgg
    10141 caaacagttg cagcccaaca tacgccgcag ccagccctgc aaccactttc aacggcaggt
    10201 tatttttcgc taccatattg tcgataaaat agctgataag cagcggccca ctgacttctg
    10261 ccgctgccgc aacccacatc atcaggaccg caatccccag cggtttacgc cacggcgaac
    10321 cgtacgctaa cagacgcttg agagtcggcc acagttggct aaaactacgc atcaacggcc
    10381 tcctcgcgaa tttccggagc gtcgtcgagc gccgcctcca gttgttgata gcgatacata
    10441 tcgcgatacc agccgctttg ttgcgccagc tcatcatgat tgccacgctg agcgatatgt
    10501 ccgtgctgca tcacaataat ttcactggct tccgtcagtg ccgaaaggcg atgggcactg
    10561 atgattaccg ttcttccctg cccccattga cgcaggttat gcaggatctg gtgctctgtg
    10621 cgtccgtcta ctgccgaaag cgcatcatca aggatgagga tttccgcgtt gactaataac
    10681 gcacgagcaa tggagatacg ctgtttttgc ccaccggaaa gcatcacgcc gcgctcgccc
    10741 acttctgtat cgtaaccttg cggtaaacgc aaaatatcgt catgcacgct ggctaaccgc
    10801 gcgacatgct caatctcttg ctgggtggca ttcgggcaac ccaacgcgat gttgttcgct
    10861 acggtgtcgg aaaaaaggaa cggtgtctgg ctaactaccg ccaggcggct acgccagcta
    10921 tcgagttgta acttcgtcag aggaatatca tgaaagcgga tatccccctc gctgacgtcg
    10981 aaatgacgct gaatgagcga caacagggta cttttgccgg aaccagtcgg cccgcaaata
    11041 cccagcatct gaccgggttt cagggcgaaa ttgacgtttt ccagcgcagg atggtcagtc
    11101 tgcggatacg tgaactggcg aatatttaca tccagttcgc cacgaccttc cggcacgggt
    11161 tcgctaccat cattcaccac cggcgcttcc gccagcatcg cgcgaatgcg gctgtacgca
    11221 gcactaccac gttccactat gttaaacatc catgccagcg ccagcattgg ccaaatcatc
    11281 agacctaaat acatcataaa actggtgagc tggcccagcg ttaaactacc ctgcaccacc
    11341 atccaactac cgccgccaat cgccagcaag ttcgccgtgc cgatggcgat gtaaatggtc
    11401 ggatcaaaac gggcatcaat acgtgccaca cgcatgtttt tcttgccggt atcttcggca
    11461 tcctcggcaa ataacgccga ctggcgatct tccagaccaa aagctttgat catgcggata
    11521 ctggtgaggc tttcctgggt gcggtcatta agactagaaa acgccgcctg tgccagctta
    11581 aagcgttcat gcaaggcatc gccgttgcgc ttgatcatga tcgccatcac tggcatcggc
    11641 aacagggcaa ataaggtcaa ctgccagcta atttgcgtag acatcataat caacacagcg
    11701 cagcccatca ccagtgaatc caccagcgtc agcacccctt ctccggcggc aaacacgacg
    11761 cgatcgacgt cattggtcgc acgagccatg aggtcacccg tgcgatgacg caggtaaaac
    11821 tcaggatgct gccggcttag ctgacggtaa taatcttcac gcagttcaac agccagttga
    11881 taagacgcac caaacagcaa tacgcgccag acgtaacgca ggagataaac cacaacggca
    11941 atcagcacca tggtggcgat ccacatcagg atctgcccgg tagtaaagtg ttgttctgtc
    12001 acgccatcga caacaatacc aaccactttt ggcggaacca gttgcagcat cgcgataatg
    12061 acaagcaagg cgacagcccc gagatagcga cgccattccc gacggaaata ccagcttaat
    12121 tgagcaaata atcgcacgcg ttgtgtcctg acctgattct ggatatttat tcgatgggta
    12181 aagaagtggt gtatttaatc tgttccatcg cgaagctgga agtgacgtcc gacagccccg
    12241 gcacgctgtt taccaggcgc ttataaaact cgtcgtagcg tttcatgtcg gcaacctgga
    12301 cgcgcatcag ataatcgtat tcaccagcca tgcgccagaa ccccagcact tctggcattt
    12361 cggtaaccac cgtgacaaag cggcaatacc attcgctgct gtgatgttgc gtttttatca
    12421 gcacaaaggc ggtcaggccg aggcctattt tttccggatc cagcagggcg actttgccga
    12481 taagaatacc gtcgtcctcc agccgtttca gacgcttcca gcagggggta gttgtcagat
    12541 taacggcttc agccagtgcc tgcaaagaga gggtgcaatc ctgctgtagt aaggccagca
    12601 gcttacggtc aattttatct aacatagccc ttccacggag aatttttttc tcttttaatt
    12661 ctattttaaa ggtgaaatcg cgacaattta ttctgtgcgg tttaaacacc acggcacaaa
    12721 atgacaaatt caacg
//
LOCUS       DACBVI010000129        12581 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.129, whole genome shotgun sequence.
ACCESSION   DACBVI010000129 DACBVI010000000
VERSION     DACBVI010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.129"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            88..1140
                     /locus_tag="IU325_17585"
     CDS             88..1140
                     /locus_tag="IU325_17585"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HAP5810813.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFTEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPTELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1216..2649
                     /locus_tag="IU325_17590"
     CDS             1216..2649
                     /locus_tag="IU325_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HAP5810814.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSGGEFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGSTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            2832..5093
                     /locus_tag="IU325_17595"
     CDS             2832..5093
                     /locus_tag="IU325_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HAP5810815.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGSIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEAPNFDVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(5163..6446)
                     /locus_tag="IU325_17600"
     CDS             complement(5163..6446)
                     /locus_tag="IU325_17600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HAP5810816.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDKVQINNVNILGRQNTFFVTNSGVQNRLESNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGNVD
                     DNDEVQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(6598..7074)
                     /locus_tag="IU325_17605"
     CDS             complement(6598..7074)
                     /locus_tag="IU325_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HAP5810817.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPVGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLARASITAMFS"
     gene            complement(7133..8422)
                     /gene="bioA"
                     /locus_tag="IU325_17610"
     CDS             complement(7133..8422)
                     /gene="bioA"
                     /locus_tag="IU325_17610"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HAP5810818.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRLDGEWDERDMVGFTRLMAAHRHEIAAVIIEPIVQGAG
                     GMRIYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQH
                     QVAAIEGQLREQLAPACDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            8509..9549
                     /gene="bioB"
                     /locus_tag="IU325_17615"
     CDS             8509..9549
                     /gene="bioB"
                     /locus_tag="IU325_17615"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HAP5810819.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            9546..10700
                     /gene="bioF"
                     /locus_tag="IU325_17620"
     CDS             9546..10700
                     /gene="bioF"
                     /locus_tag="IU325_17620"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HAP5810820.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKLSAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVITAMMAKEDRIVADRLSHASLLEAASLSPSPLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLEEIQQVTQQHDGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSNTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSWSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDSLLEVLHDNG"
     gene            10687..11442
                     /gene="bioC"
                     /locus_tag="IU325_17625"
     CDS             10687..11442
                     /gene="bioC"
                     /locus_tag="IU325_17625"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HAP5810821.1"
                     /translation="MTTVNKQAIAAAFGRAAAHYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQKQGRYPLTYHLFLGVIARE"
     gene            11435..12112
                     /gene="bioD"
                     /locus_tag="IU325_17630"
     CDS             11435..12112
                     /gene="bioD"
                     /locus_tag="IU325_17630"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAP5810822.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALM"
BASE COUNT         3014 a         3157 c         3405 g         3005 t
ORIGIN      
        1 aatcgttgag ctaaagtcat tagagatgct ttgcccttaa tgtaaccata tcgcaataag
       61 ttatgttttt aaattgaggg cattattatg aaaaaaatac cctgcgtgat gatgcgaggt
      121 ggaacctcga ggggcgcgtt cctgttagcg gaacatctac ccgaagatca aacgcagcgc
      181 gataaaatat tgatggcaat tatgggttcc ggtaacgatc tggaaattga cggtattggc
      241 ggcggtaatc cgctgaccag taaagtcgcc attattagcc gctccagcga tccgcgtgca
      301 gatgtcgatt atctgtttgc tcaagttatc gtccatgagc aacgtgtcga taccacgcct
      361 aactgcggca atatgctgtc tggcgttggg gcattcgcca ttgaaaatgg tttgattgca
      421 gcgacttcgc cagttacccg cgtacgtatc cgcaacgtca atacgggtac gttcaccgaa
      481 gctgatgtgc aaacgccaaa tggtgttgtc gagtacgagg gcagcgccag aattgacggc
      541 gtaccgggta ctgccgcacc ggttgcgctc actttcctga atgccgccgg aaccaaaacc
      601 ggaaaagtct tcccgacgga taatcagatt gattattttg acgatgtccc ggtgacctgt
      661 atcgatatgg cgatgccagt tgtcattatt cctgctgaat atctgggaaa aacaggctat
      721 gaattaccga cggagttgga tgccgacaaa gcattattag ctcgcattga atctatccgt
      781 ctacaagcgg gtaaagcaat gggcttaggc gatgtcagta atatggttat ccctaaacct
      841 gtacttattt ctccagcgca gaaaggcggg gcaattaatg tgcgttattt tatgccgcat
      901 tcttgccatc gcgctttggc gataaccggt gctattgcta tttccagtag ttgtgcattg
      961 gaaggcaccg tcacccgaca aatcgtccct tctgtaggat acggcaatat caatattgaa
     1021 caccccagtg gtgcgctcga cgttcattta agtaatgaag gtcaggatgc cacgacgtta
     1081 cgcgcatctg ttattcggac gaccagaaaa atattttccg gtgaagttta tcttccctga
     1141 aaaaattcgt tgtcaggata aggacaatca ataaaggact tctgtatgag tcatacagaa
     1201 agaacaggat attaaatgaa taagaaatcg ttatggaagc taattctgat attagcaatc
     1261 ccatgtatta ttggctttat gccagctcca gcaggattaa gcgaactggc gtgggtgctt
     1321 tttggtattt acctggcggc cattgtgggg ctggttatca agcctttccc ggaacctgtc
     1381 gtactgttaa ttgccgtcgc cgcatcgatg gtagtggtgg gtaacttatc aggtggggaa
     1441 tttaaaacca ccgccgtatt aagcggttac tcttccggta ccacctggct ggtgttttct
     1501 gcgtttactt taagcgccgc gtttgtaacc acaggcttag gtaaacgtat tgcctatctg
     1561 ctgattggta aaattggtag cactaccctg ggtctgggtt acgttacggt atttctcgat
     1621 ctggtactgg ctccggcaac accgtctaac accgcgcgtg cgggcggcat tgtgttaccg
     1681 atcatcaaca gcgtggcggt ggctttgggg tcagaaccgg aaaaaagtcc gcgtcgtgtc
     1741 ggacattacc tgatgatgtc catttacatg gtcaccaaaa ccaccagcta tatgttcttt
     1801 accgcaatgg cggggaacat tctggcgctg aaaatgatca acgacattct gcacctgcaa
     1861 attagctggg gtggatgggc gctagccgcc ggattgcctg gcatcattat gctgctggtc
     1921 accccgctgg tgatttacac catgtatcca ccagaaatta agaaggtgga taacaaaacc
     1981 atcgctaaag cgggccttgc cgaactggga ccgatgaaaa tccgcgaaaa aatgctgctc
     2041 ggtgtctttg tgctggcgct gctgggctgg attttcagta agtctctggg ggttgatgaa
     2101 tccaccgtgg caatcgttgt tatggcaact atgctgctgc tgggtatcgt tacctgggaa
     2161 gacgtggtta aaaataaagg cggctggaat accttaatct ggtacggcgg tattatcggc
     2221 ttaagctcct tattatcgaa agttaaattc ttcgaatggt tagctgaagt ctttaaaaat
     2281 aacctggcat ttgatggtca cgggaacgtt gctttcttcg ttattatttt cctcagcatc
     2341 atcgtgcgtt atttcttcgc ttctggtagt gcctatatcg ttgccatgtt accggtattt
     2401 gccatgctgg cgaatgtctc cggcgcaccg ttaatgttaa ccgcgctggc actgttgttc
     2461 tctaactcct atggcggcat ggttactcac tatggcggcg cggcaggtcc ggtcatcttt
     2521 ggcgtgggtt acaacgatat taaatcctgg tggttggtcg gtgcggtact gacgatatta
     2581 accttcctgg tgcatatcac cctcggcgtg tggtggtgga atatgctgat cggctggaac
     2641 atgctgtaaa tatactcgtc atacttcaag ttgcatgtgc tgcgtctgcg ttcgctcacc
     2701 ccagtcactt acttatgtaa gcgcctgggg attcactctc ttgtcgcctt cctgcaactc
     2761 gaattattta gagtatatcc atttattatc tttctgcgca ctttccggtg cgcagatatc
     2821 tggagcattt gatgatcaag ttatctgaaa aaggcgtgtt tctcgccagt aataacgaaa
     2881 taattgccga agaacatttc accggcgaaa ttaaaaaaga agaagccaaa aaaggcacta
     2941 ttgcctggtc tattctctct tctcataata cgtccggaaa tatggataaa cttaaaatta
     3001 agtttgattc attagcctct cacgatatta cctttgttgg tattgtacag accgctaaag
     3061 cgtccggtat ggaacgtttc ccgctgccgt atgtgctgac caactgccat aactcactct
     3121 gcgccgtcgg cggcactatt aacggtgatg atcatgtttt tggtttatcg gcagcccagc
     3181 gttatggcgg tatttttgtg cctccgcaca ttgcggtcat ccatcaatat atgcgtgaga
     3241 tgatggcagg cggcggcaaa atgatcctcg ggtcagacag ccatacccgt tacggtgcgt
     3301 tagggacaat ggcggttggc gagggcggcg gcgagttggt aaaacaactg cttaatgaca
     3361 cctgggatat cgactatccg ggcgtggttg cggtgcatct gaccggaaaa cctgcgccgt
     3421 atgtggggcc gcaggatgtg gcgctggcta tcatcggtgc cgtgttcaaa aacggttacg
     3481 tcaaaaacaa agtgatggag ttcgtaggtc ccggtgttgc agcgctctct accgatttcc
     3541 gcaacagcgt tgacgtgatg accactgaaa cgacctgttt aagttctgtc tggcaaaccg
     3601 atgaagaagt ccataactgg ctggcgttgc acggtcgcgg ccaggattac tgccagctta
     3661 accctcaacc aatggcgtac tacgatggct gcatcagcgt tgatttaagc gccatcaaac
     3721 caatgattgc gctgccgttc cacccgagca acgtctatga aatcgacacg ctgaaccaga
     3781 acctgaccga cattctgcgt gagattgaaa ttgagtccga acgcgtggcg cacggtaaag
     3841 ccaaactctc gctgctggat aaagtcgaaa acggtcgcct gaaagtgcag caggggatta
     3901 tcgcgggctg ttctggcggt aactacgaaa acgtcatcgc ggcggcgaat gcactgcgcg
     3961 gtcaatcctg tggcaatgac accttctcgc tggcggttta cccgtcatca cagccggtgt
     4021 ttatggatct cgccaaaaaa ggtgtggtcg cagatttgat tggcgcaggc tcaatcatca
     4081 gaaccgcgtt ctgcggccca tgctttggcg cgggcgatac gccaatcaac aacggtttga
     4141 gtattcgcca caccacgcgt aacttcccga accgcgaagg ctctaagcca gctaatgggc
     4201 agatgtcagc ggtggcgttg atggacgctc gttctattgc ggcgactgcg gcaaacggtg
     4261 gctatttaac ctctgccagc gagctggatt gctgggacaa cgtgccggag tacgccttcg
     4321 atgtaacgcc gtataaaaac cgtgtttatc agggctttgt gaaaggggcg acccagcaac
     4381 cgctgattta cggaccgaac attaaagact ggccagaatt gggtgcgctg actgacaata
     4441 tcgtcctcaa agtgtgctcg aagatcctcg acgaagtgac caccaccgac gaactgattc
     4501 cttccggtga aacctcttct tatcgttcaa atccgattgg tctggcggag tttaccctgt
     4561 cacgccgcga tccaggttat gttggtagaa gtaaagcgac tgctgagctg gaaaatcagc
     4621 gtctggcggg aaatgtcagc gagctgacag aggtgtttgc gcgcattaag cagattgctg
     4681 gtcaggagca tattgatccg ctgcaaactg aaattggcag catggtttat gcggtgaaac
     4741 caggcgatgg gtctgcgcgt gaacaggcgg cgagttgtca gcgtgtgatt ggcggtctgg
     4801 cgaatattgc cgaggagtac gcgactaaac gctatcgttc taacgtcatc aactggggga
     4861 tgttaccgct gcagatggcg gaagcaccga actttgacgt aggtgattac atttacatcc
     4921 ctggcattaa agcagcgctg gataatccgg gtacgacgtt taaaggttat gtgatccatg
     4981 aagatgcgcc ggtaacggaa attacgcttt acatggaaag cctgacggcc gaagagcgcg
     5041 agattatcaa ggcgggtagt ttgattaact tcaataaaaa ccgtcagatg taaaaaaagg
     5101 cgctatgaaa tatagcgccc agtttcatta acccgatggt tcagactatc gggttttctt
     5161 ttttacttct tcgcctctgc aaccactttg ctacccacgc cgcggttatt gtattcccac
     5221 atgcggttgt agttagtgtc attcagattg cgctgtactt cgtcgttatc atcaacgttg
     5281 ccagtattac ccgcaaacgg acgattggaa atcaccgcat cggcccacgg tttcgccgtg
     5341 ttaaaacctt cgttgatggc gctatcacgg atcactacct gaccgttggt attggcatca
     5401 acatccagcg agcggcccag ttgcgccacg ccatccccgg aagcattgaa acggctgttt
     5461 acggcgagga aaccgtagta aatgttggac agcgtagccg gtgcaaacac atacgcttct
     5521 tgctgggtac gggagttcac cacgcggaat tcggtgttat cgaacaccac tgcgccgcga
     5581 ccagaaacga tatccacatc cccttcaatg tagctgttag tcaccagggt acgcggctga
     5641 cggttgcttt ccagacggtt ttgcacaccg ctgttggtga caaagaaggt attctggcga
     5701 ccgagaatat tgacgttatt gatctgcact ttgtcgccat cagtacgcag tgccaccgcc
     5761 ggatggttac ccgcatctac gctatcgccc agtgtgtttt cgatggtcag gttttgcagt
     5821 tgcaggccat tgttttgtga ccagaagacc gcagaacaga gcacgccaat gctgtcgctg
     5881 cgtttgctct ggcagctatc gtacatatac cacgctggtt tacccggcat atatttgccg
     5941 cgtgggttga cgtcgtgacg ccagtcggct gggctcatgc ccccatcaag ggaaagccca
     6001 attttcacat caatcggttt ttcacctgta ccgtacagag taattccacc cggagcggca
     6061 gggacataca ccgttccctg atactcacca ggcatcacgg caatatactg gcgcttgttg
     6121 gtacgcttga taattgccgc atctaccgcc gcctgaatcg tggtatgcgt tacaccttga
     6181 gtacccgccg ggccgacaac aaagtcaggt tgtgcaggca gggtaatcgg ggaaggattc
     6241 cacgctgcag cacctggtgt cagggatgca aaatagtgtt gagcatcgaa attctgcgct
     6301 tcttttgccg acagaatcgg gcgagaagag gtaccaggcg cggtttgatc agaaggacgt
     6361 tgatcgggcg gtgttgagct acaggcggtc agcgtcacgc caaaagccaa tgccagcgcc
     6421 agacgggaaa ctgaaaatgt gttcacaggt tgctccgggc tatgaaatag aaaaatgaat
     6481 ccgttgaagc ctgctttttt atactaactt gagcgaaacg ggaaggtaaa aagacaaaaa
     6541 gttgttttta atacctttaa gtgataccag atggcattgc gccatctggc agagtgatta
     6601 actaaacatc gcagtaatcg aggctctcgc cagagagtga aaatgaacgt taaacccgac
     6661 catcgcgccg ctggcacctt catcgacatc aatacgttct acatccagcg cgtgaacggt
     6721 aaaaatgtag cgatgggttt cgcctttcgg cggcgctgcg ccatcgtatc cggttttacc
     6781 aaagtcggta cgcgtctgca aaacgccgtc tggcattgct accagaccag agccaaaccc
     6841 ttgcggtaat acgcgggtat ccgcgggtaa attaacaact acccagtgcc accagccgga
     6901 gccagttggc gcatccgggt cgtagcaggt gacaacaaaa cttttcgttc ccacaggaac
     6961 atcatcccac gccagatgcg gtgaaatatt atcgccatcg taacccatgc cgttaaagac
     7021 atgacgatgc ggcagcttat cgccatcgcg taaatcatta ctgatgagtt tcatgagccc
     7081 tcctttcttg tttgcagaaa gtgtagccag aaaccctcac gctaacttcc cgttattggc
     7141 aaaaaaatgt ttcatcctgc accgcacgat taaccgctgc ggtcagacgc tgcaactgtt
     7201 gcgggagaat aatatagggc ggcatcaggt aaatcagttt gccaaaaggc cggatccaga
     7261 caccctgttc gacaaagaat ttttgcagcg ctgccatatt caccggacga gtggtttcga
     7321 ccacgccgat tgcccccagt acgcgcacat cggcaaccat ttcggcgtca caggcggggg
     7381 caagttgctc gcgtagctgc ccttcaatag ccgccacctg atgctgccag tcgccagatt
     7441 cgagaatcgc caggctggcg tttgctgctg cgcaggccag cggattgccc ataaaagttg
     7501 gcccatgcat aaagcagccg gcttcgccgt tactgatggt ttccgcaacc tcgcgcgtgg
     7561 tgagcgtggc ggaaagggtc attgtgccgc cggttaaggc tttaccgaga cacaaaatat
     7621 ccggcgcgat ttctgcatgt tcacaggcaa acagtttgcc ggtacgacca aatccggtgg
     7681 cgatctcgtc ggcaatcagc aagatacctt cgcgatcgca cattttgcgg attcgtttta
     7741 accattccgg atggtagatg cgcatcccgc ctgcgccctg gacaatcggt tcaatgatca
     7801 ctgcggcgat ttcatgacga tgcgccgcca tcaggcgggt aaagcccacc atatcgcgtt
     7861 catcccattc accatccagg cggctttgcg gggcgggagc aaacaagttt tctggcagat
     7921 agcctttcca caggctgtgc attgagttgt ccggatcgca caccgacatc gcgccaaagg
     7981 tatcgccatg ataaccattg cggaaggtca gaaaacgctg gcgcgcttcg cctttggcct
     8041 gccagtactg caacgccatt ttcatcgcca cttctaccgc tacggagccg gagtccgcga
     8101 gaaaaacgca ctccagcggt tgcggcgtca ttgccaccaa tttgcggcac agctcaatgg
     8161 cgggcgcatg ggtgataccg ccaaacatca catgcgacat agcatcaatt tgcgatttca
     8221 tcgccgcatt aagctgcggg tgattgtaac cgtggatcgc cgcccaccag gacgacatac
     8281 cgtcaacaag gcgtctgccg tcagacaaaa ttagctcgca accctcggcg ctcgccaccg
     8341 gataaaccgg tagcggggag gtcatggatg tgtatgggtg ccagatatgg cgttggtcaa
     8401 aggcaagatc gtccgttgtc ataatcgact tgtaaaccaa attgaaaaga tttaggttta
     8461 cgagtctaca ccgaattaac aacaaaaaac acgttttgga gaagccctat ggctcaccgc
     8521 ccacgctgga cattgtcgca agtcacagaa ttatttgaaa aaccgttgct ggatctgctg
     8581 tttgaagcgc agcaggtgca tcgtcagcat ttcgatccac gtcaggtgca ggtcagcact
     8641 ttgctgtcga ttaagaccgg agcttgtccg gaagattgca aatactgccc gcaaagctcg
     8701 cgctacaaaa ccgggctgga agccgagcgg ttgatggaag ttgaacaggt gctggagtcg
     8761 gcgcgcaaag cgaaagcggc aggatcgacg cgcttctgca tgggcgcggc gtggaagaat
     8821 ccccacgaac gcgatatgcc ctacctggaa caaatggtgc agggagtaaa agcgatgggg
     8881 ctggaggcgt gtatgacgct gggtacgttg agtgaatctc aggcgcagcg ccttgcgaac
     8941 gccgggctgg attactacaa ccacaacctc gacacctcgc cggagtttta cggcaatatc
     9001 atcaccacgc gtacctatca ggaacgcctc gatacgctgg aaaaagtgcg cgacgccggg
     9061 atcaaagtct gctcgggcgg cattgtgggc ttaggcgaaa cagtaaaaga tcgcgccgga
     9121 ttattgctgc aactggcaaa cctgccaacg ccgccggaaa gcgtaccaat caacatgctg
     9181 gtgaaggtga aaggcacgcc gcttgctgat aacgatgatg tcgatgcctt tgattttatt
     9241 cgcaccattg cagttgcgcg gatcatgatg ccgacctctt acgtacgcct ttctgccgga
     9301 cgcgagcaga tgaacgaaca gactcaggcg atgtgcttta tggctggcgc aaactcgatt
     9361 ttctacggtt gcaaactgct gaccacgccg aatccggaag aagataaaga cctgcaactg
     9421 ttccgcaaac tggggctaaa tccgcagcaa actgccgtgc tggcgggcga taacgaacaa
     9481 cagcagcgtc tggagcaggc gctgatgacc ccggacactg acgaatatta caacgcggca
     9541 gcactatgag ctggcaggag aaaatcaacg cggcgctcga tgcgcggcgt gctgccgatg
     9601 ccctgcgtcg ccgttatcca gtggcgcaag gagccggacg ctggctggtg gcggacgatc
     9661 gccagtatct gaacttttcc agtaacgatt atttaggttt aagccatcat ccgcaaatta
     9721 tccgtgcctg gaagctgagt gcggagcaat ttggcgtcgg tagcggcggc tccggtcacg
     9781 tcagcggtta tagcgtggcg catcaggcgc tggaagaaga actggccgag tggctgggct
     9841 attcgcgggc actgctgttt atctctggtt ttgccgctaa ccaggcagtt attaccgcga
     9901 tgatggcgaa agaggaccgt attgttgccg accggcttag ccatgcctca ttgctggagg
     9961 ctgcaagttt aagcccgtcg ccgcttcgcc gttttgctca taacgatgtc actcatctgg
    10021 cgcgactgct tgcttccccc tgtccggggc agcaactggt agtgacagaa ggcgtgttca
    10081 gcatggacgg cgatagtgcg ccactggagg aaatccagca ggtaacgcaa cagcacgatg
    10141 gctggttgat ggtcgatgac gcccacggca cgggcgttat cggggagcag gggcgtggca
    10201 gctgctggct gcaaaaggta aaaccagaat tgctggtggt gacttttggc aaaggatttg
    10261 gcgtcagcgg ggcagcggtg ctttgctcca atacggtggc ggattatctg ctgcaattcg
    10321 cccgccatct tatctacagc accagtatgc cgcccgctca ggcgcaggca ttacgtgcgt
    10381 cgctggcggt cattcgcagt gatgagggtg atgcacggcg cgaaaaactg gcggcactca
    10441 ttacgcgttt tcgtgccgga gtgcaggatt tgccgtttac gcttgctgat tcatggagcg
    10501 ccatccagcc attgatcgtc ggtgataaca gccgtgcgtt acaactggca gaaaaactgc
    10561 gccagcaagg ttgctgggtc acggcgattc gcccgccaac cgtacccgct ggtactgcgc
    10621 gactgcgctt aacgctaacc gcagcgcatg aaatgcagga tatcgatagt ctgctggagg
    10681 tgctgcatga caacggttaa taaacaagcc attgcagcgg catttggtcg ggcagccgcg
    10741 cactatgagc aacatgcgga tctacagcgc cagagtgctg acgtcttact ggcaatgctc
    10801 ccacagcgta aatacaccca cgtactggat gcgggttgtg gacctggctg gatgagccgt
    10861 cattggcggg aacgtcacgc gcaggtgacg gcgttagatc tctcgccgcc aatgcttgtt
    10921 caggcacgcc agaaggatgc cgcagaccat tatctggcgg gagatatcga atccctgccg
    10981 ttagcgactg caaccttcga tcttgcatgg agcaatctcg cggtgcagtg gtgcggtaat
    11041 ttatccacgg cactccgcga gctgtatcgg gtggtgcgcc ccggaggcgt ggtcgcgttt
    11101 accacgctgg tgcagggatc gttacccgaa ctgcatcagg cgtggcaggc ggtggacgag
    11161 cgtccgcatg ctaatcgctt tttaccgcca gatgaaatcg aacagtcact gaacggcgtg
    11221 cattatcaac atcatattca gcccatcacg ctgtggtttg atgatgcgct cagtgccatg
    11281 cgttcgctga aaggcatcgg tgccacgcat cttcatgaag ggcgcgaccc gcggatatta
    11341 acgcgttcgc agttgcagcg attgcaactg gcctggccac aaaaacaggg gcgatatcct
    11401 ctgacgtatc atcttttttt gggagtgatt gctcgtgagt aaacgttatt ttgtcaccgg
    11461 aacggatacc gaagtgggga aaaccgtcgc cagttgtgca cttttacaag ccgcaaaggc
    11521 agtaggctac cggacggcag gttataaacc ggtcgcctct ggcagcgaaa agaccccgga
    11581 aggcttacgt aatagcgacg cgctggcgtt acagcgcaac agcagcctgc agctggatta
    11641 cgcaacggta aatccttaca ccttcgcaga acccacttcg ccgcacatca tcagcgcaca
    11701 agagggcagg ccgatagaat cattggtaat gagcgccgga ttacgcgcgc ttgaacaaca
    11761 ggctgactgg gtgttagtgg aaggtgctgg cggctggttt acgccgcttt ctgacacttt
    11821 cacttttgcg gattgggtaa cacaggaaca actgccggtg atactggtag ttggtgtgaa
    11881 actcggctgt attaatcacg cgatgttgac ggcacaggca atacaacacg ccggactgac
    11941 tctggcgggt tgggtggcga acgatgtcac gcctccggga aaacgtcacg ctgaatatat
    12001 gaccacgctc acccgcatga ttcccgcgcc gctgctgggc gaaattccct ggcttgcaga
    12061 aaatccagaa aatgcggcaa ccggaaagta catcaatctt gccttaatgt agtcatcctg
    12121 tacttggtta aattgcgagc gagatcgcgt cttcgattga ctgcaattta accaattaaa
    12181 ttctaaaaaa atcacgaaaa aaattttacc tccgcatcat gcggtgaatg tgggaattgc
    12241 ccaggcggcg ggggataggg gctgcagaca gttatccact attcctgtgg ataaccatgt
    12301 gtattagagt tagaaaacac gaggcaagcg agagaatacg cggcttgcac gcgaattggc
    12361 gttaaagacg gctcaaagaa atatctttta ttttttaact ggttagataa ttgcaatggc
    12421 agtcactgaa gaggcatctc ttgccataaa actgtcatca ctcatcttga caaatgttaa
    12481 aaaagccgtt gctttgggga taacccggta aggccggagt tttatctcgc cacagagtaa
    12541 attttgctca tgattgacag cggagtttac gctgtaacag a
//
LOCUS       DACBVI010000130        12417 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.130, whole genome shotgun sequence.
ACCESSION   DACBVI010000130 DACBVI010000000
VERSION     DACBVI010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.130"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            259..2160
                     /locus_tag="IU325_17635"
     CDS             259..2160
                     /locus_tag="IU325_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810823.1"
                     /translation="MVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDYE
                     LAADVYNINVTSPTGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEAI
                     LHAFNATYQQIRKNISEFARCHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPEDL
                     CTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKIS
                     KDIESGIPGYWQGQVLIPFIIDPVTKERDQIHPEDIHLTAKDFEASKDNISKDEWENL
                     HALKEKRLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSIT
                     PPLKNYNERLHNTNLFNPKYQKKAVMNYMEYLVTCPLDEQQMSSLLYERDDLSAIDFS
                     LLMKTIKAFSFGGDLQALASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNHQ
                     QDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNDDGSTRVGGEAWLNKGCSTYQQL
                     YNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVEG
                     TWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKAFTLRGGTLNINIPGRSALLLGKT
                     GEPPNYLYL"
     gene            2497..3474
                     /gene="csiD"
                     /locus_tag="IU325_17640"
     CDS             2497..3474
                     /gene="csiD"
                     /locus_tag="IU325_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HAP5810824.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYASNHYQTHQ"
     gene            3494..4762
                     /gene="lhgO"
                     /locus_tag="IU325_17645"
     CDS             3494..4762
                     /gene="lhgO"
                     /locus_tag="IU325_17645"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HAP5810825.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4785..6233
                     /gene="gabD"
                     /locus_tag="IU325_17650"
     CDS             4785..6233
                     /gene="gabD"
                     /locus_tag="IU325_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HAP5810826.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERATILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGVPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLEKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            6247..7527
                     /gene="gabT"
                     /locus_tag="IU325_17655"
     CDS             6247..7527
                     /gene="gabT"
                     /locus_tag="IU325_17655"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HAP5810827.1"
                     /translation="MSSNKELMQRRSQAVPRGVGQIHPIFADRAENCRVWDVEGREFL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            7765..9165
                     /gene="gabP"
                     /locus_tag="IU325_17660"
     CDS             7765..9165
                     /gene="gabP"
                     /locus_tag="IU325_17660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HAP5810828.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            9186..9848
                     /gene="csiR"
                     /locus_tag="IU325_17665"
     CDS             9186..9848
                     /gene="csiR"
                     /locus_tag="IU325_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HAP5810829.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9849..10298)
                     /gene="kbp"
                     /locus_tag="IU325_17670"
     CDS             complement(9849..10298)
                     /gene="kbp"
                     /locus_tag="IU325_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HAP5810830.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPAAASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10382..10540)
                     /locus_tag="IU325_17675"
     CDS             complement(10382..10540)
                     /locus_tag="IU325_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HAP5810831.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10723..11022
                     /locus_tag="IU325_17680"
     CDS             10723..11022
                     /locus_tag="IU325_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HAP5810832.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            11032..11556
                     /gene="ygaP"
                     /locus_tag="IU325_17685"
     CDS             11032..11556
                     /gene="ygaP"
                     /locus_tag="IU325_17685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HAP5810833.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRHEQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVILGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(11603..12007)
                     /gene="stpA"
                     /locus_tag="IU325_17690"
     CDS             complement(11603..12007)
                     /gene="stpA"
                     /locus_tag="IU325_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HAP5810834.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         3122 a         3038 c         3170 g         3087 t
ORIGIN      
        1 ttaaatacat tagaatatct aaactgtata acaaatatta ttaattcttg tggagtttat
       61 cctcaggatt taaaacacag agaaattata tcaacttttc acgcagaaaa agttattaat
      121 gatctgttaa aaaacgatta taaaatttcc ctttttccag atacaactta ccgagagttg
      181 aataaagcag cacagcgtag cattacagcg ccagacagga taggagaagg aaaaacatgg
      241 gtttatcaac gagatacaat ggttgaaaga ggtgataaca gcggtgttca tcagtatggt
      301 ccagctgaac attttactca cattatatct gacaaaccgt ccccaaaaga taagtatgtt
      361 gcatatgcta ttaacattcc tgactatgaa ctggcagctg atgtatataa tattaacgta
      421 acgtcaccta ccggccagca agaaacattt aaaatattaa tcaatccaga acatctacgg
      481 caaacacttg agcgtaaatc tcttactgct gttcagaaat cacaatgtga aatcatcacc
      541 cccaaaaaac ctggcgaagc gattcttcat gcttttaatg ccacctatca acaaatcagg
      601 aaaaatatat ctgagtttgc acgttgtcat tatgggtata tacaaatccc tcccgtaacc
      661 actttccgcg ccgacggacc agaaactccc gaagaagaaa aaggttactg gtttcacgct
      721 tatcaacccg aagatctttg tactatccat aatccaatgg gagatttgca ggattttatc
      781 gcattggtta aagatgccaa aaaatttggt atcgatatca tccctgatta tacatttaac
      841 tttatgggaa tcgggggtag tggtaaaaat gatctggatt atccctctgc cgatatacga
      901 gcgaagatca gtaaagatat agaaagtggt atccctggct attggcaagg tcaggtcttg
      961 attcccttta ttatagatcc agtaacaaaa gaacgtgacc aaatccatcc agaagatata
     1021 catctcactg caaaagactt cgaagcaagt aaagataata tttctaagga tgaatgggaa
     1081 aacctccatg cattaaaaga aaagcgttta aatggaatgc ctaaaacaac acccaaaagt
     1141 gaccaggtta ttatgttgca aaatcaatac gttcgtgaaa tgcgaaaata tggcgtacga
     1201 ggtttacgtt atgatgcagc aaaacactca aaacatgaac aaatagagag gtcgataacc
     1261 ccaccgctta aaaattataa tgagcggttg cacaatacaa atttatttaa tccaaaatat
     1321 caaaaaaaag ctgttatgaa ttacatggaa tacctggtaa cttgtccgtt agatgaacaa
     1381 caaatgtcat cgctgcttta tgaaagagat gatttaagcg ccattgattt ttcattgctc
     1441 atgaagacga taaaagcctt ttcattcggt ggagatctcc aggcccttgc atcaaaaccg
     1501 ggctccacaa tctcaagcat cccatcagaa agacggatat tgattaacat caaccacgat
     1561 ttccctaaca atggtaatct tttcaatgac tttttattta accatcagca agatgaacaa
     1621 ttagcaatgg catatatggc cgctctcccg ttcagcaggc ctttagttta ctgggatggt
     1681 caggtattaa aatcaacgac tgagattaaa aatgatgatg ggtctactcg tgtcggcggt
     1741 gaggcgtggc ttaataaagg ttgctctacc tatcagcagc tctacaatga attccacgca
     1801 ttatatatag ataaagcagg aatatggagc gcatttgagg gtgtatttgc aactaaaaac
     1861 gttctggcct ttagtcgtgg ggattctgtg aacattaatc actctcctca tgatggacta
     1921 gttataataa ataaaggaaa cgaagaagtt gaaggtacct ggcctaacaa attgcaacct
     1981 ggaatatata aaaacatggg gagtaatagc gttaacatta ttattaataa tacccgaaaa
     2041 attatccccc ctggtaaagc atttacgctt agaggcggaa ctctaaatat caatattcct
     2101 gggcgtagcg ctcttctttt agggaaaact ggagaaccgc cgaactatct ctatttgtaa
     2161 tttaatgtta tatctgcccc gctaaaacgg ggcagataat atgtttagtt tactaacggt
     2221 cattttgcag tgaagccatt taccgttttt tatcgaccag ataatctgtt ctctgatggt
     2281 aatcctcccc taacctgttg ctttagttat tcatttcctg tctcactttg ccttaatacc
     2341 ctacgttaaa tgttaccaat ttgttgcttt tgatcacaat aagaaaacaa tatggcgcat
     2401 ttgtgcgcat ttttcagaaa tgtagatatt tttagattat ggctacgaaa tgagcatcgc
     2461 catgtcaccc tacatctcat aagaggatcg cttctgatga atgcactgac cgccgtacaa
     2521 aataacgctg tcgattcagg ccaggactat agcggattca ctctcatccc gtcggcgcaa
     2581 tccccgcgtc tgctggaact caccttcacc gaacagacga ccaaacagtt tctcgaacag
     2641 gttgccgaat ggcccgtgca ggcgctggag tacaaatctt ttctgcgttt tcgggtaggc
     2701 aaaattcttg acgatctgtg tgcgaaccag ctgcaaccat tgctgttgaa gaccctgcta
     2761 aaccgtgctg aaggtgcgct gttgatcaat gcggtgggtg tcgatgatgt gaagcaagcg
     2821 gatgagatgg tgaagctggc aacggcggta gcgcatctga ttggtcgctc aaatttcgat
     2881 gcgatgagcg gtcagtatta cgcgcgattc gtggtgaaaa atgtcgataa ctcagacagc
     2941 tatctgcgtc agccgcaccg cgtaatggag ctgcacaacg acggcaccta cgtcgaagag
     3001 atcaccgact atgtgctgat gatgaaaatc gacgagcaga acatgcaggg tggaaattcg
     3061 ttgctgctgc atctcgatga ctgggaacat ctggaccact atttccgcca cccgctggcg
     3121 cgtcgcccga tgcgctttgc cgcgcctccg agcaaaaacg tcagcaaaga tgttttccat
     3181 ccagtgttcg acgttgatca acagggtcgc ccggtgatgc gttatatcga ccagttcgtc
     3241 cagccaaaag acttcgaaga aggcgtgtgg ctgagcgagc tttccgatgc cattgaaacc
     3301 agcaaaggca ttctttctgt gcccgttccc gttggcaaat tcctgttgat taacaatcta
     3361 ttctggctgc acggtcgcga ccgctttact ccgcacccgg atctgcgccg tgaactgatg
     3421 cgtcagcgtg gctatttcgc ttacgcctct aaccactacc agacgcatca gtaagcgcaa
     3481 aggaattgag cggatgtatg attttgtgat tattggcggc ggcatcatcg gcatgtcgac
     3541 cgccatgcaa ctgattgatg tttatccgga tgcccgcatt gcgttgctgg aaaaagagtc
     3601 cggtccggcc tgtcatcaga caggccacaa cagcggcgtg atccatgccg gggtctatta
     3661 cacgcccggc agcctgaagg cgcagttttg cctggcggga aaccgcgcca ccaaagcctt
     3721 ttgcgatcaa aacggcattc gctatgacaa ctgcggcaag atgctggtcg ccacctctga
     3781 acttgaaatg gaacggatgc gcgcgttgtg ggaacgcacg gcggcgaacg gtatcgagcg
     3841 cgagtggtta aacgccgagg aactgcgcga gcgcgaaccg aatatcaccg ggctcggcgg
     3901 catttttgtg ccgtccagcg gcattgtcag ctatcgcgaa gtgacggcgg cgatggcaaa
     3961 aatcttccag gccagaggcg gcgagattat ttataacgcc gaagtcagcg ccctcagtga
     4021 gcataaaaac ggcgtggtga tacgtacccg tcagggcggt gaatatgaag catcgacgct
     4081 gattagctgt tccgggctga tggctgaccg gctggtgaaa atgctcggcc tcgaaccggg
     4141 ctttattatc tgcccgttcc gtggcgagta tttccgcctt gcgccggagc ataaccagat
     4201 tgttaaccac ctgatttacc ccattcccga ccccgcgatg ccatttttgg gcgtgcatct
     4261 cacccgcatg atcgatggca gcgtgaccgt cgggccaaac gcggtgctgg cttttaaacg
     4321 cgaaggctat cgcaagcgcg atttctcgtt tagtgacacg ctggagattt taggctcgtc
     4381 ggggattcgc cgggtgctgc aaaaccatct acgctcagga ttgggcgaga tgaaaaactc
     4441 gctgtgcaaa agcggctatc tgcggctggt gcaaaagtat tgtccccggc tttcgttaag
     4501 cgatctccag ccctggccag caggtgtgcg ggcgcaggcg gtatcgccgg acggcaagct
     4561 gattgacgat tttctgtttg tcaccacccc gcgcacgatc cacacctgca atgcgccctc
     4621 cccggcagcg acatcagcaa ttcctattgg tgcgcatatt gtcagcaagg tacaaacgct
     4681 gttggcaagc cagagtaacc ccggacgcac gctgcgagcg gcacgtagtg tggatgcctt
     4741 acacgccgca tttaatcaat aacctttgaa aacaggacgt aacaatgaaa cttaacgaca
     4801 gtaacttatt ccgccagcag gcgttgatta acggggaatg gctggacgcc aacaatggcg
     4861 aggtcatcga cgtcaccaat ccggcgaacg gcgacaagct gggtagcgta cccaaaatgg
     4921 gcgctgatga aacccgcgcc gctatcgacg ccgccaaccg tgctctgccc gcctggcgtg
     4981 cgctcaccgc caaagaacgc gccaccattc tgcgcaactg gttcaatttg atgatggagc
     5041 atcaggacga tttagcgcgc ctgatgaccc tagaacaggg caaaccgctg gctgaagcga
     5101 aaggcgaaat tagctacgcc gcctccttta ttgagtggtt tgccgaagaa ggcaaacgca
     5161 tttatggcga caccattcct ggtcatcagg ccgataaacg cctgattgtt atcaagcagc
     5221 cgattggcgt caccgcggct atcacgccgt ggaacttccc ggcggcgatg attacccgca
     5281 aagccggtcc ggcgctggcg gcaggctgca ccatggtgct gaagcccgcc agtcagacgc
     5341 cgttctctgc gctggcgctg gcggagctgg cgattcgcgc aggcgttccg gctggcgtat
     5401 ttaacgtggt caccggttcg gcgggcgcgg tcggtaacga actgaccagc aatccgctgg
     5461 tgcgcaagct gtcgtttacc ggctcgaccg aaattggccg ccagttaatg gaacagtgcg
     5521 cgaaagacat caagaaagtg tcgctggagc tgggcggcaa cgcgccgttt atcgtctttg
     5581 acgatgccga cctcgacaaa gccgtggaag gcgcactggc ctcgaaattc cgcaacgccg
     5641 ggcaaacctg cgtctgcgcc aaccgtttat acgtgcagga cggcgtgtat gaccgctttg
     5701 ccgaaaaatt gcagcaggcg gtgagcaaac tgcacatcgg cgacgggctg gagaaaggcg
     5761 tcactattgg gccgctgatt gatgaaaaag cggtagcaaa agtggaagag catatcgccg
     5821 atgccctgga gaaaggcgcg cgcgtggttt gcggcggtaa agcacatgaa cgcggcggca
     5881 acttcttcca gccgaccatt ctggtagacg ttccggccaa cgccaaagtg tcgaaagaag
     5941 agacgttcgg ccccctcgcc ccgctgttcc gttttaaaga tgaagccgat gtgattgcac
     6001 aagccaatga caccgagttt ggtcttgccg cctatttcta cgcccgtgat ttaagccgcg
     6061 tcttccgcgt gggcgaggcg ctggaatacg gcatcgtcgg catcaatacc gggattattt
     6121 ccaatgaagt ggccccgttc ggcggcatta aagcctcggg tctgggtcgt gaaggttcga
     6181 agtatggcat cgaagattac ttagaaatca aatatatgtg catcggtctt taactggaga
     6241 acgcgaatga gcagcaataa agagttaatg cagcgccgta gtcaggcagt tcctcgtggc
     6301 gttgggcaaa ttcacccgat tttcgctgac cgcgcggaaa actgccgggt gtgggacgtt
     6361 gaaggccgtg agtttcttga tttcgcgggc gggattgcgg tgctcaatac cgggcacctg
     6421 catccgaaag tggttgcggc ggtggaagcg cagttgaaaa agctgtcgca cacctgcttc
     6481 caggtgctgg cttacgaacc gtatctggag ctgtgcgaga ttatgaatca gaaggtgccg
     6541 ggagatttcg ccaaaaaaac gctgctggta acgaccggtt ctgaagcggt ggagaacgcg
     6601 gtgaaaatcg cccgcgccgc caccaaacgc agcgggacca tcgcctttag cggtgcatat
     6661 cacgggcgca cgcattacac gctggcgctg accggcaagg tgaatccgta ctctgcgggc
     6721 atgggactga tgcctgggca cgtttatcgc gcgctttatc cttgcccgct gcacggcatc
     6781 agtgaagatg acgctatcgc cagcattcac cgaattttta aaaatgacgc tgcaccggaa
     6841 gatatcgccg ccatcgtgat tgagccggtt cagggcgaag gcggtttcta cgccgcgacg
     6901 ccagccttta tgcagcgttt acgcgcgctg tgtgacgagc acgggatcat gctgattgcc
     6961 gatgaagtgc agagcggcgc ggggcgtacc ggcacgctgt ttgcgatgga gcagatgggc
     7021 gttgcgccgg atcttaccac ctttgcgaaa tcgatcgcgg gcggcttccc gctggcgggc
     7081 gtcaccgggc gcgcggaagt gatggatgcc gtcgctccag gcggtctggg cggcacctat
     7141 gccggtaacc cgattgcctg cgtggctgcg ctggaagtgt tgaaggtgtt tgagcaggaa
     7201 aatctgctgc aaaaagccaa cgatctgggg cagaagttga aagacggatt gctggcgata
     7261 gccgaaaaac acccggagat cggcgacgta cgcgggctgg gagcgatgat tgccatcgag
     7321 ctgtttgaag atggcgatca cagcaaaccg gacgccaaac tcaccgccga gatcgtggcg
     7381 cgtgcccgcg ataaaggcct gattcttctc tcctgcggcc cgtattacaa cgtgctgcgc
     7441 atccttgtac cgctcaccat tgaagacgct cagatccgtc agggtctgga aatcatcagc
     7501 cagtgttttg ctgaggcgaa gcagtagatc cgtacccatg ccggaggcga cgctgcgcgt
     7561 cttgtccggc ctacgcgaat ccaggtcgga taaagcgttt acgctgcatc cgacaatctg
     7621 tacgtgaaca ggaataaatc tatgtcggcc ggataaggcg gagccgctct ccggtaaaaa
     7681 gaaccaataa caattacacg cgtgacccga cgcgggaaat gtcggggcgc tctcccaagt
     7741 gacacacttt cgagaggatt caggatgggg caatcatcgc aaccacatga gttaggcggc
     7801 gggctgaagt cacgccacgt caccatgttg tctattgccg gtgttatcgg cgcaagtctg
     7861 tttgtcggtt ccagcgtcgc tatcgccgaa gcgggcccgg cggtattact ggcctatctg
     7921 ttcgccggat tactggtggt tatgattatg cggatgttgg cggaaatggc ggttgccacg
     7981 cccgacacgg gttcgttttc cacctatgca gataaagcta ttggtcgttg ggcgggctat
     8041 accatcggct ggttgtactg gtggttttgg gtactggtta tcccgctgga agccaacatc
     8101 gccgccatga tcctgcactc gtgggttcct ggcattccca tctggttatt ttccctcgtc
     8161 attaccctcg ccttaactgg cagtaattta ttaagcgtta aaaactacgg cgaatttgag
     8221 ttctggctgg cgctgtgcaa agtcatcgct atcctggcct ttattttcct tggtgcagtc
     8281 gcaattagcg gtttttaccc ttatgccgaa gtgagcggga tctcaagatt gtgggatagc
     8341 ggcggcttta tgcccaacgg cttcggcgcg gtattaagcg cgatgttgat caccatgttc
     8401 tcgtttatgg gcgcagaaat tgtcaccatt gccgccgcgg aatctgacac gccggaaaaa
     8461 catattgtcc gcgccaccaa ctcggttatc tggcgtattt ctatcttcta tttgtgctct
     8521 atttttgtcg tcgtggcatt aataccgtgg aatatgccgg ggctgaaagc cgttggatct
     8581 tatcgctcgg ttctggagtt gctcaatatt ccccatgcga aattaatcat ggactgcgtg
     8641 atattacttt ccgtaaccag ttgcctgaac tcggcgctgt ataccgcttc cagaatgctc
     8701 tactccttaa gccgtcgcgg tgatgctccc gcggtaatgg gtaaaatcaa ccgcagtaaa
     8761 accccgtatg tggcggtatt actctcgacc ggtgcggcat ttttaacggt ggtggtgaat
     8821 tattacgcgc ctgcgaaagt gtttaaattt ctgatcgaca gctccggcgc tatcgccctg
     8881 ctggtttatt tagtcatcgc cgtttcacaa ttgcggatgc gcaaaattct gcgagcagaa
     8941 ggaagcgaaa ttcgcctgcg gatgtggctt tatccgtggc tcacctggct ggtcatcggc
     9001 tttattacct ttgtgttggt agtgatgcta ttccgcccgg cgcaacagtt agaagtgatc
     9061 tccaccggct tattagcgat agggattatc tgtaccgtgc caattatggc tcgctggaaa
     9121 aagctggtat tgtggcaaaa aacacccgtt cataatacgc gctgataatg atcaggagtc
     9181 acaccatgac cattacgtct ctggatggct atcgctggct gaagaacgat attattcgcg
     9241 gtaattttca accggatgaa aaattacgga tgagtttgct tacatcgcgt tatgcacttg
     9301 gcgttgggcc gttacgggaa gctctttcgc aactggtggc ggaacggctg gtcacggtgg
     9361 tgaatcaaaa agggtatcgg gtggcgtcta tgtcagagca ggagctgctc gatattttcg
     9421 acgcccgcgc caatatggaa gcgatgctgg tcagtctggc gattgcccgc ggtggcgatg
     9481 aatgggaggc agacgttctc gcaaaagcgc atctgctcag taagctggag gcctgtgacg
     9541 ccagtgagaa aatgcttgat gagtgggatc tgcgtcatca ggcgtttcat acggcaattg
     9601 tggcgggctg tggttctcac tatttgctgc aaatgcgtga acggttgttt gatctggcgg
     9661 cgcgctatcg atttatctgg ctgcggcgaa cagtgctttc ggtggagatg ctggaggata
     9721 aacacgatca gcaccagacc ctgactgcgg cggtactggc gcgagatacc gcgcgcgcca
     9781 gtgagttaat gcgccagcat ttactgacgc caattcccat tatccagcag gcgatggctg
     9841 gcaattaact actcttcagg aatacgcaac acttgccccg ggtaaatttt atccgggctt
     9901 ttcagcatcg gtttattcgc ttcgaagatt ttattgtaca gattggcgtt accgtagacc
     9961 tgtttagaaa tggcgctaag agtgtcgcca gacttaacgg tataaaactg gctggcagcg
    10021 gctggtgtcg ccgttttcac ctgatcatcg acactggcaa taccggaaat attccccacc
    10081 gcaacaagga ttttctcctt cgcctcctga cttaagccgt cacccgtgac cgtcgctttg
    10141 ccgtcggcga tttgaatgtt cactttatcg gcatcaggta taccggtttt gttcagatgc
    10201 tcctgcacct tcttcgcctg atcgtcttta tcgtgctgac ctgtaaccgc gtcccagagt
    10261 ttttctccgg catctttcac aaaattgaac agacccatag ctacctcatt cgttaacgga
    10321 aacactcaga aagtgtagca gaggaacgcg gcgcgttaac gccgcggggg tgaacacagg
    10381 tttaatcgcg ggtttgcacc cagaaagcgt gaatcaaacc ggggatatac ccaagcagtg
    10441 tcagcagaat attaataatg aacgcccaac cgaacccttt accgagcagc acgccgagcg
    10501 gcggcagaat gatggtgatg acgattctcc agaaacccat atgtactccc tataagaaaa
    10561 ttactcattg tttaaaaaga gattttatct cttaagcgta gtaattttag cggagcctgc
    10621 cagtttcagc ttacgctttt actctcattt acgctgctga tgctggcata tgttatattt
    10681 ttagtttaga gtttacttaa tttagaaaat gcttaaataa ctatgactga actcgcgcaa
    10741 ttacaggcca gtgccgaaca ggcagcggcc ttattgaaag caatgagcca ccctaaacgg
    10801 ttgctgattc tgtgcatgct tagcggttct cccggcacca gtgcgggaga gctgacgcgc
    10861 attaccggac taagtgcctc tgcaacatca cagcatctcg ctcgtatgcg ggacgaaggg
    10921 cttatcgaca gccaacggga tgcccaacgc attctatatt ccattaaaaa tgaggcggta
    10981 aatgccatta tcgccaccct gaaaaatgtc tattgtccgt aaggagtcac tatggctttg
    11041 acaaccattt cgccgcatga tgcacaagaa ttaatcgcac gcggcgcaaa gttaatcgat
    11101 attcgtgacg ctgatgaata tcttcgtgag catattcctg aagcagatct ggctccatta
    11161 tccgtgctgg aacagtcagg tcttccggct aaattacgtc acgagcaaat tatattccac
    11221 tgccaggcag gtaaacgcac cagtaataac gccgataaat tagcagcgat tgccgccccc
    11281 gcagaaatct ttttactcga agatgggatt gatggctgga aaaaagcagg attaccagta
    11341 gcggtaaata aatctcaacc cttgccgtta atgcgccagg tgcagatcgc tgcgggtggt
    11401 ttaatattaa tcggcgttat actcggttat accgtaaata gcggtttctt cttattaagc
    11461 ggctttgttg gtgccgggtt actgtttgca ggaattagcg gtttttgtgg gatggcaagg
    11521 ttgttagata agatgccgtg gaaccaacga gcttgagaag cgacgccgga cgcgccatag
    11581 cggcggcatc cggcctcagt aattagatca ggaaatcgtc gagagattta ccttctgcca
    11641 gcgcctgagc gattggcttc ggtgtacggc cctgaccggt ccaggtttta gtttcaccgt
    11701 taacatcggt gaatttatat ttcgccggac gcggctggcg ttttttacca gcgcgtgggg
    11761 cggccgcgga gctattaccc aataactctt ccgggttaat tccgtcagct ttcatcagct
    11821 ccagccaggt gctaattttt tcctggcgct ctgccagttc acgctgctgc tgttcttctt
    11881 cttcacgtct ttctttagtg acaaccctga atttttcgag catttcttca agaacgtcaa
    11941 tggagaattc gcgagccatc gcacggaggg tgcgaatgtt atttaaactt tgtaacatta
    12001 cggacataat aaagaaaacc ttttaacgcc aaaacaaaaa agtatttcgt cgcgtagtgt
    12061 aagcgatcac ttattatatt tccacaaaat ttaatctatt tacagtcctg cgcgagatta
    12121 tttcagctgt tattttctga aactctcaga ataatttcaa gattatatat tagccatttt
    12181 gatgagtaat ttcggttatt tgtgagcaga gcggcgctct ctgctcgcta attccgcaag
    12241 cctgacaaaa caaaaatgaa taaaaatgca atttaaccga ataaatggaa acacccctca
    12301 ctggtgcttt ctgctgtcaa tgtacatttt ctggcataaa aaatgctgaa aatgacccgg
    12361 tcacgacata caccgaattc tctacgctta tagcgttacc tcacccctaa accttca
//
LOCUS       DACBVI010000131        12376 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.131, whole genome shotgun sequence.
ACCESSION   DACBVI010000131 DACBVI010000000
VERSION     DACBVI010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.131"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..354
                     /locus_tag="IU325_17695"
     CDS             <1..354
                     /locus_tag="IU325_17695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HAP5810835.1"
                     /translation="QRRSPDIVAELATIKDKSDDEWWRALLQSRNDHLRQTALRNAHT
                     PMLLTTLTESQDRSLAINNPQLAADVKTVWLKEEPSLLLFVDQPALSQLRDLVKTGAT
                     RKIRSEARHRLEEKQ"
     gene            351..434
                     /locus_tag="IU325_17700"
     CDS             351..434
                     /locus_tag="IU325_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810836.1"
                     /translation="MTQRPWSKLQRKTHNIAALKIIARRSE"
     gene            complement(512..1423)
                     /gene="rdgC"
                     /locus_tag="IU325_17705"
     CDS             complement(512..1423)
                     /gene="rdgC"
                     /locus_tag="IU325_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HAP5810837.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSTAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            1548..2456
                     /locus_tag="IU325_17710"
     CDS             1548..2456
                     /locus_tag="IU325_17710"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HAP5810838.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGLRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYHRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(2599..3783)
                     /gene="araJ"
                     /locus_tag="IU325_17715"
     CDS             complement(2599..3783)
                     /gene="araJ"
                     /locus_tag="IU325_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HAP5810839.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNVLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFFGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     VG"
     gene            complement(3909..7052)
                     /gene="sbcC"
                     /locus_tag="IU325_17720"
     CDS             complement(3909..7052)
                     /gene="sbcC"
                     /locus_tag="IU325_17720"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HAP5810840.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAVEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNAWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGATLRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIHYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRMQQLTPILETLPQSDELPHSEETVALENWRQVHEQCLALQSQQQTLQQQE
                     ALAAQSLQKAQAQFDTALQDSIFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTQQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESMFAVK"
     gene            complement(7049..8251)
                     /gene="sbcD"
                     /locus_tag="IU325_17725"
     CDS             complement(7049..8251)
                     /gene="sbcD"
                     /locus_tag="IU325_17725"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HAP5810841.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGAQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITEQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            8441..9130
                     /gene="phoB"
                     /locus_tag="IU325_17730"
     CDS             8441..9130
                     /gene="phoB"
                     /locus_tag="IU325_17730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HAP5810842.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPISHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            9188..10483
                     /gene="phoR"
                     /locus_tag="IU325_17735"
     CDS             9188..10483
                     /gene="phoR"
                     /locus_tag="IU325_17735"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HAP5810843.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            10890..12209
                     /gene="brnQ"
                     /locus_tag="IU325_17740"
     CDS             10890..12209
                     /gene="brnQ"
                     /locus_tag="IU325_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HAP5810844.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
BASE COUNT         2735 a         3198 c         3309 g         3134 t
ORIGIN      
        1 cagcgaagat ctccagatat tgttgcggaa ctggcgacga taaaagataa atcagatgat
       61 gaatggtggc gtgcattgct gcaaagtcgc aatgatcatt tgcgccaaac ggcattgcgc
      121 aacgcacata ccccgatgtt gttaacgaca ttgacggagt ctcaggatcg gtcgctggct
      181 attaataatc cacagctggc tgccgatgtg aaaacggtgt ggctaaaaga ggagccatca
      241 ttacttttat ttgtcgatca acccgctctt tcgcagttac gggatttagt gaaaaccggg
      301 gcaacgcgga aaattcgtag tgaagcgcgt caccggcttg aggaaaaaca atgacacaac
      361 gcccctggag caagttacag cgtaaaacgc ataatattgc tgcgctaaaa attattgccc
      421 gccgtagcga ataattatat gcctggtgta gcttcgtacg ccggataaga cgcggcaggc
      481 gtcgcatccg gcattaaagg taaatcagca attaacgttg tgcttcgcca cctaatcctt
      541 caatcaggtt ttgaattaac gctgccagtt caccagtcat caggatgaaa tcggcatcaa
      601 aacgctgggc gaaatcttca cggtcgatat cttcgttttg atcgcgcagc tcgtcgcaga
      661 acttcagacg cttgagcgaa ccatcgtcgc acatcacaaa ctgaatgcgc tgctgccagt
      721 cgagcgccag tttagtcacc acttttccgg cttcaatgtg gttggtgatc tcttcgctgg
      781 tcagatcttg tttcttcgcg cggatcacgc cgccatcttc cagcagtgat ttcagctcgg
      841 cttcatcaag cagctggaag ccctgtgctg tgctgccgga gcgtacccat tcggtcagcg
      901 tcagttcaat cggattttcc atgctcaatg gtacaaccgg taacgacccc aggcttttac
      961 gcagtaatgc cagcgtatct tccgcttttt tggcactggc gcagtccacc ataatcaaac
     1021 cgttaaccgt gtcgatccac atcattgtct ggctaaaacg gctgaaagca cgcggcagca
     1081 gagaatgcag cacttcgtct ttcagcgaat ctttttcggt tttcttcagc ttacgcgcct
     1141 gttccgcttc cagtttggcg attttcgctt ccagcgcctg tttaatcacc ggagacggga
     1201 ggattttttc ttctttgcgc gcgcagataa caatttgacc attggcaacg tgcgttaacg
     1261 catcgctgtg cgatcccatc ggaggaaccc agcccatctt tgccatgtcc tggctgccgc
     1321 atggggtaaa tgccatcgag gctagctgtt tttccatctc ttctgcacgc agcgaaatct
     1381 cgcggctaag acggtaaacc attaaatttt tgaaccacag catgataatt tccacggcct
     1441 tgtcgttaaa tttagcgggc atgataacga attgtcggcg ggcttgcatt gccaatccgg
     1501 ttgtccgtct ctacgctatt gatattgaaa aaaataagga gagtaccgtg cgtataggta
     1561 tcgatttagg cggcaccaaa actgaagtga ttgcactggg cgatgcaggg gagcagttgt
     1621 accgccatcg tctgcccacg ccgcgtgatg attaccggca gactattgaa acgatcgcca
     1681 cgttggttga tatggcggag caggcgacag ggctgcgcgg aacggtaggt atgggcattc
     1741 ctggctcaat ttcgccttac accggcgtgg tgaagaatgc caattcaacc tggctcaacg
     1801 gtcagccatt cgataaagac ttaagcgcgc ggttgcagcg ggaagtgcgg ctggcaaatg
     1861 acgccaactg tctggcggtt tcagaagcag tagatggcgc ggcagcggga gcgcagacgg
     1921 tatttgccgt gattatcggc acgggatgcg gcgcgggcgt ggcattcaat gggcgggcgc
     1981 atatcggcgg caatggcacg gcaggtgagt ggggacacaa tccgctaccg tggatggacg
     2041 aagacgaact gcgttatcgc gaggaagtcc cttgttattg cggtaaacaa ggttgtattg
     2101 aaacctttat ttcgggcacg ggattcgcga cggattatca tcgtttgagc ggacatgcgc
     2161 tgaaaggcag tgaaattatc cgcctggttg aagaaagcga tccggtagcg gaactggcat
     2221 tgcgtcgcta cgagctgcgg ctggcaaaat cgctggcaca tgtcgtgaat attctcgatc
     2281 cggatgtgat tgtcctgggg ggcgggatga gcaatgtaga ccgtttatat caaacggttc
     2341 cgcagttgat taaacaattt gtcttcggcg gcgaatgtga aacgccggtg cgtaaggcga
     2401 agcacggtga ttccagcggc gtacgcggcg ctgcatggtt atggccacaa gagtaaaaaa
     2461 accaggcaat tggctcatca tgcctgatgc gacgcttgcc gcgtcttatc aggcctgcaa
     2521 aatgtgccag aaccgcgtag ggcggataag gcgttcacgc cgcatccggc aataagcaac
     2581 gtgctccgtg actgcaatct accccactgg tttcgccagc accggactat ccgccgcttg
     2641 ctggcgctta tagcgaccat acagcagcaa cgacgacatc gcagcaaacg aaagcagggc
     2701 ggcaggtagc gccacgtaat tatatgccag ccccagcgtc agcatcatac caccgcaata
     2761 tgcgccgacg gcgctaccga ggttaaacgc tatttgccca cctgcggcac ctaataactc
     2821 tccgcctttg gcattttgca gtaacaatat ttgcagtggt gctgaaaggg caaataatcc
     2881 cgcgcaacaa ataaatgcaa aaataagcga cgttgttttc atgccgccga agaaaaagag
     2941 catcagcagt gccagcacaa taataaagtc agttactgcc gcaatgcgca gtggtgaata
     3001 acgtcctgaa atccggccac ttagcacatt ccccagcacc atccctagcc caactaacat
     3061 cataataaaa gtcatcgccg tttccgaaaa accggaaata aacatcatgt atggctttac
     3121 gtagctgaac caggcaaata cacctgcgtt gccaaacatg gtggcggcga aaattaacca
     3181 cggggccggg ctgcgcaaaa agtgaaattg ttcgcgtagc ttccctttcg cctcgtcgcg
     3241 aatatccggc acccaaaaat agaccgatgc catcaccgca atattaaaaa cagcgatcaa
     3301 taaaaaggtg taacgccagc taaattcctg acttaaatac gttcccagcg gaatgcccag
     3361 caaattggcg actgtcatcc cggaaaccat ccccgccacg gcggcggtga ctttgccggg
     3421 tttgataatt tttgataaca cgatcgcgcc gacgccaaaa aatgcgccat gcggaaagcc
     3481 ggataccagt cgaccaatgg cgagcatcag gtaagacgaa gagagcgtga acatggcgtt
     3541 gccaatgacg cacaacgcca ccagaaacaa taagatatgt ttgagtgagt agcggctgga
     3601 aaagagtgcg atgattggcg caccgaccac cacccccagc gcataatacg agatcatatg
     3661 cccggcagca ggaatcgaaa ttcctacgtt atgcgccagc tccgtgagca cgcccataat
     3721 gccaaattcg gccatcccca aaccaaacgt gcccagagcc aaagataaaa tgactttttt
     3781 cataccacca tcacaaccac cagaacggct accaatcacc gtagccaata aagtgatagc
     3841 ttgcaggata acgaccagca aggccaagag ataattcgcc cctctgtatt cattatcctg
     3901 ctgaatagtt atttcactgc aaacatactt tccagtttgc tgtagcccag gccgttgatc
     3961 tttttcactt tgatctgcac cggaatacgc tctttcatcg cttctacgtg gctaatcaca
     4021 ccgatggttt tgccgctggc gttcagggca tccagcgcat caagggcggt atccagcgtt
     4081 tcgctatcca gcgtgccaaa accttcatca aggaacagcg agtcaatacg cgttttatgg
     4141 ctgaccagat ctgaaagcgc cagtgccagc gccagactga cgaggaaact ttcgccgcca
     4201 gaaagggtac gggtgtcgcg taccgcatct gcctgccagg tatcaacaac ctcgacttcc
     4261 agcgcctcgc tggctttgcg ctgtaacaga tagcgcccgt gcagccgggt aagttgctga
     4321 ttagcgagat ggactaaatt atccagcgtc agcccctggg caaacttgcg gaatttatcg
     4381 ccctctttgg aacctattag cgaattcaga tatccccagt cctcaacctg ctgcgtcatt
     4441 tgagcaattt gctgcattaa ggtttgttgt tgctgacggt tgtctgcatc ctgcttcagc
     4501 tgctggcgaa tctcgccctg actggtggtg ttttcacgca acttttgctg agtttgcgct
     4561 aactcttgct gaatctgctc caccgtcaca gtgagagcca acccgtcagg tcggtgttgc
     4621 tgatgctgtg ccagcgtttc agctgtctga gtgaccagcg tttgcgcctg acggcgctgg
     4681 ttttccagat tctgtttcag ctgttccagc tgcgttagcg tctgctcatc cattaacgcc
     4741 gcaagaaatg cctgctgatc gtcaaaaatg ctgtcctgta gcgcggtgtc aaactgcgcc
     4801 tgggcttttt gcagactttg cgctgccaga gcctcctgtt gctgcaacgt ttgctgctgg
     4861 ctttgcaacg cgaggcattg ttcgtgaact tgccgccagt tttccagcgc tacagtttct
     4921 tcgctgtgcg ggagttcatc actttgcggc aacgtttcca gaatcggcgt cagttgctgc
     4981 atacggtttt gcagcgcggt taattcgttc tggcgttgct gccagctctg cgcttcttgc
     5041 tgacgcgtcg ccaaccagct ctcttcttca tcttcctgtg gcaatgtcag tgcataaccc
     5101 gccaatgccg ttaagagctg ctgctggcgt tgttcaattt gctgttgata atggataatt
     5161 tgctgattat gcgcggcaat ctgcccttgt aattcatgcc gttggctgag taaccgcagc
     5221 tggcgttcgt gctcatcttg tgcatccagc cacggttgaa tatcgtcctg tggctgcaag
     5281 gtgatattga ggctggccgt gacggcttgc cattgttgag taagtgcttg ctcatcttgt
     5341 cggaggcttt gcgcttcgtt ttcatcacgc tgaagttgct ttgttaaggc gtccagttgc
     5401 ccacgtagcg tcgcaccttc ttcaccgagc tttttaactt cgttttccag cgccagtaat
     5461 cgagactgat taacgccagg ctccagcgcc tgatacgcct cgaccgccgg gtggctggtg
     5521 gaaccacaaa gtggacaagg ctgacccgcc tgtaactgtg cacgttgagc ttccagcgtt
     5581 ttgatgcgcg cttcctgctc gcaaatggtt ttcacatcgg caagttgctg cgtcttttct
     5641 ttataacgct ggcgcatttc gttaagtgcg gcgttacgtt gcgtctgttc ttgcgtgaca
     5701 ttctggatag cgacctgtaa ttgcgccagg cgtttttgtt ggggaacaat ctgtccatgc
     5761 agcgcgacca ggcgctgacg cagtgggcgt tgctcagcat gttgcgccag ggcgctagca
     5821 acttcatcgg cggttaacgt caacgtgatc gccgcaagcg cattaagttt ttgctctgca
     5881 tgggttaact gttgctgcca ttgccgcaga tgctcgcgat cgctggtttg ttgggagaac
     5941 tgcgcacgcc aacccgccag ttcgttgttc cactgacgga agcggtcgtg ttcctgtaac
     6001 caggcattca gactttgttg ctgctgctgt aattctgctg actgcttcgc cgcgtggtgg
     6061 cgaatgctcg cgcgaagcgc cattgtgctc tgtaagcgag tatttacttc ttcaatctgc
     6121 tggcgagtat gcgccagcgc cgcgctgtgt tctgcgatgc gttcccagtg cggacgaaga
     6181 tttcgtgccg gttgtgccag actgagcgcc gccagttgag gttgcgcttt ttcttctacg
     6241 gctaacgcct gttgcaaggc ctgttgacgg cggctggctt cttgctgcaa ttcgtccaga
     6301 cgcgttaacc agtttagtga ttgttgttct tgctgctgcg cggtaagtaa ctgtttttct
     6361 tcgtcagtaa gtacctgcaa actcgctgtc agcgattgca cttgttctgg cgtgagcaac
     6421 gcgacgccgc tggcctgcgc ttgcagcttc tccagctctg tgcgggctga tttgtgctgc
     6481 tcaaaaacca tcgccgagat ttgcccgtag atttcagtgc cagttaactc ctcgagcaat
     6541 tccgcgcgtt ctttgggttt ggcattcagg aaggcagcaa attgcccctg cgaaagcagc
     6601 atcgaacggg tgaagcgccc gtaatccagt ccggttaacg tcgccgtcag ttccagctta
     6661 tctttcactt tgtcggcgag aattttgcca tcggcgcagc gcgccagctc tacgcgtggc
     6721 acctgcaaat taccgtccgg ctggttacgc gcccgattct ggctccagaa cgcgcgatac
     6781 gcttcacctt tcacttcaaa ctccacctcc gccagacatt cggcggtatc gcgggtcatg
     6841 agatcatttt gcgattgtga aacgttagaa aggcgcggag tttcgtgata cagcgccaga
     6901 caaatggcgt ccagcagggt ggttttcccc gcaccggttg ggccggtaat ggcaaacagc
     6961 ccgttgctgg cgaacggctc gcgggtgaaa tcaatcttcc attcgccttt taatgagttc
     7021 aggtttttca ggcgcaggct gagaattttc atgcttcgtg ttctccggcg agggtatgca
     7081 acgtcgtggt gaaaagatgc tgcagacgtt gctgctgcga ttcatccagt tcttccagtg
     7141 ccagacggcg attgaacacc tcttcgacgc tgagttcgct gagggtttca cgctgttggc
     7201 tggctaacac tcgctcgcgc tgttcacgac tccggcgtac cagcaatact tcgacaggca
     7261 atgattcggt taatgcctgg attttgcgct gaatatcatg cagatactca tcagtagtga
     7321 tttcgatatc cagccagacc ggtggctcct gtgatacatc gcgccactgt tccagctgtt
     7381 cagtaatcga cgccagatcg cctttcagca ccgccatggg ttgcgttacc ggtacgttca
     7441 ggttttccac gctctctaat ttgccgtttg aaaatgtcac cagatggaca tatttactct
     7501 taccgcattc atcaaaactc agtggaatgg gggagccgca atagcgaaca tgttccatgc
     7561 cgccaataat ctgcgcgcgg tgaatatgcc caagcgcgat gtagtcggct ggcggaaagt
     7621 tttgtgccgg aaacgcgtcc agcgtgccaa tatagatgtc acgcacggca tcacttttac
     7681 tggcacccac agttgttaaa tgtcccgtgg cgatgatggg cagaggctga gcgccgcgca
     7741 gtttgcaggc atcggcatag tgttgttgat aataatcagt aatagccgcc agcaaatgct
     7801 gctgtttttc aataccgtta agccccgcct ggctggtaat aatgtcacgt gggcgtaaaa
     7861 acggaatggg gcacagcact gcccctggcg tcccgtcgcg acgaggcaag atttgcggcg
     7921 catgtccggc gctggcgacc acggtcgtat tgaggaacgc catgatatcg cgcgattcat
     7981 tcagcgtggc gaccgagtca tggtttcctg ccagtaccac cagatgacag ccagtttgct
     8041 gtaaattgac aacaaaacgg ttgtataacg tgcgggcgta actggggggc gagccggtat
     8101 cgaaaacatc accggcaaca ataatcgcat ccacctgatg ggtttgtgct gtctccagca
     8161 gccagtcaag aaaagcctga tgttcagctt cgcggctttt actgtagaag ttctggccga
     8221 gatgccagtc tgaggtgtga aggatgcgca taacggttcc ctggcgaaaa agcatgggcg
     8281 cgattatacc caaacagatg tgccatttgc ttttttctgc gccacggaaa tcaataacct
     8341 gaagatatgt gcgacgagct tttcataaat ctgtcataaa tctgacgcat aatgacgtcg
     8401 cattaatgat cgcaacctat ttattacaac agggcaaatc atggcgagac gtattctggt
     8461 cgtagaagat gaagcaccaa ttcgcgaaat ggtctgcttc gtgctcgaac aaaatggctt
     8521 tcagccggtc gaagcggaag attatgacag tgctgtgaat caactgaatg aaccctggcc
     8581 ggatttaatt ctccttgact ggatgttacc tggcggctcc ggtatccagt tcatcaaaca
     8641 cctcaagcgc gagtcgatga cccgggatat tccagtggtg atgttgaccg ccagagggga
     8701 agaagaagat cgcgtgcgcg gccttgaaac cggcgcggat gactatatca ccaagccgtt
     8761 ttcgccgaag gagctggtgg cgcgaatcaa agcggtaatg cgccgtattt cgccaatggc
     8821 ggtggaagag gtgatcgaga tgcaggggct aagcctcgac ccgatatctc accgggtgat
     8881 ggcgggcgaa gagccactgg agatggggcc gacagaattt aaactgctgc acttttttat
     8941 gacgcatcct gagcgcgtgt acagccgcga gcagctgtta aaccacgtct ggggaaccaa
     9001 cgtgtatgtg gaagatcgta cggtcgatgt ccacattcgt cgcctgcgta aagcactgga
     9061 gcccggcggg catgaccgca tggtgcagac cgtgcgcggt acaggatatc gtttttcaac
     9121 ccgcttttaa cgccttgctc atcggacgca gagcagggct tatgatttct taactggagt
     9181 atcttacgtg ctggaacggc tgtcgtggaa aaggctggtg ctggagctgc tactttgctg
     9241 cctcccggct ttcatcctgg gtgcattttt tggttacctg ccctggtttt tgctggcatc
     9301 ggtaacagga ctgcttatct ggcatttctg gaatttattg cgcctttcat ggtggctgtg
     9361 ggtggatcgt agtatgaccc cgccaccggg acgtggtagc tgggaaccgc tgctatacgg
     9421 attacaccag atgcagttgc gaaataaaaa acgccgccgc gaactgggca atctgattaa
     9481 acgctttcgt agcggcgcgg agtcgctgcc cgacgcggtg gtgctgacca cggaagaggg
     9541 cggtattttc tggtgcaacg gtctggcgca acagattctt ggcttgcgct ggccggaaga
     9601 taacgggcag aacatcctta acttgctgcg ttacccggag tttacgcaat atctgaaaac
     9661 gcgtgatttt tctcgcccgc ttaatctggt gctcaacacc gggcggcatc tggaaattcg
     9721 cgtcatgcct tatacccaca aacagttgct gatggtggcg cgtgatgtca cgcaaatgca
     9781 tcaactggaa ggggcgcggc gtaacttttt tgccaacgtg agccatgagt tacgtacgcc
     9841 attgactgtg ttacagggtt acctggagat gatggatgag caaccgctgg aaggcgcggt
     9901 acgtgaaaaa gcgttgcaca ccatgcgcga gcagacccag cggatggaag ggctggtgaa
     9961 gcaattgctg acgctgtcga aaatagaagc cgcaccgacg catttgctca atgaaaaggt
    10021 tgatgtgcca atgatgctgc gcgttgttga gcgcgaggct cagactctga gtcagaaaaa
    10081 acagacattt acctttgaga tagataacgg cctcaaggtg tctggcaacg aagatcagct
    10141 acgcagtgcg atttcgaacc tggtctataa cgccgtaaat catacgccgg aaggcacgca
    10201 tatcaccgta cgctggcagc gagtgccgca cggtgccgaa tttagcgttg aagataacgg
    10261 accgggcatt gcaccggagc atattccgcg cctgaccgag cgtttttatc gcgttgataa
    10321 agcgcgttcc cggcaaaccg gcggtagcgg attagggtta gcgatcgtga aacatgcggt
    10381 gaatcatcac gaaagtcgcc tgaatattga gagtacagta ggaaaaggaa cacgtttcag
    10441 ttttgttatc ccggaacgtt taattgccaa aaacagcgat taatccgcct ttgtcatctt
    10501 ttattgccat aagccagccg atgctggctt attttctttg cagtcaaaat acgggcgtta
    10561 gattttacaa cggttggtga ttttttgttc gcatgattag ccatgtcttt ttcacggaaa
    10621 tagtgtttta tactgtttgg tgatttctta tcgctatata cctctggttt ttagatccct
    10681 tcttgcttta aaacgttata agcgtttaaa ttgcgcttca ggtgctgtca tactgactgc
    10741 attcacgcgg taaatcgaaa aactattctt cgccgcgcct ggttgggagt atttcccgct
    10801 aaaattgttt aaatataccg ctgtatcatc cccagggatt ggcacaaaaa tttaacgtta
    10861 caacaccaca tccacaggca gtatgattta tgacccatca attaagatcg cgcgatatca
    10921 tcgctctggg ctttatgaca tttgcgttgt tcgtcggcgc aggtaacatt attttccctc
    10981 caatggtcgg cttacaggca ggcgaacacg tctggactgc ggcattcggc ttcctcatta
    11041 ctgccgttgg cctgccggta ttaacggtag tggcgctggc aaaagttggc ggcggtgttg
    11101 acagcctcag cacgccaatc ggtaaagtcg ctggcgtact gctggcaacg gtgtgttacc
    11161 tggcggtggg gccgcttttc gctacgccgc gtacagctac cgtttctttt gaagtgggca
    11221 ttgcgccgct gacgggtgat tccgcactgc cgctgtttat ctacagcctg gtttatttcg
    11281 ctatcgttat tctggtttcg ctctatccgg gcaaactgct ggataccgtg ggcaacttcc
    11341 ttgcgccgct gaaaattatc gcgctggtca tcctgtctgt tgccgcgatt gtctggccgg
    11401 cgggttctat cagcacggcg actgaggctt atcaaaacgc tgcgttttct aacggcttcg
    11461 ttaacggcta tctgaccatg gatacgctgg gcgcaatggt gtttggtatc gttattgtta
    11521 acgcggcgcg ttctcgtggc gttaccgaag cgcgtctgct gacccgttat accgtctggg
    11581 ctggcctgat ggcgggtgtt ggtctgactc tgctgtacct ggcgctgttc cgtctgggtt
    11641 cagacagcgc gtcgctggtc gatcagtctg caaacggtgc ggcgatcctg catgcttacg
    11701 ttcagcatac ctttggcggc ggcggtagct tcctgctggc ggcgttaatc ttcatcgcct
    11761 gcctggtcac ggcggttggc ctgacctgtg cttgtgcaga attcttcgcc cagtacgtac
    11821 cgctctctta tcgtacgctg gtgtttatcc tcggcggctt ctcgatggtg gtgtctaacc
    11881 tcggcttgag tcagctgatt cagatctctg taccggtgct gaccgccatt tatccgccgt
    11941 gtatcgcact ggttgtatta agttttacac gctcatggtg gcataattcg tcccgcgtga
    12001 ttgctccgcc gatgtttatc agcctgcttt ttggtattct cgacgggatc aaagcatctg
    12061 cattcagcga tatcttaccg tcctgggcgc agcgtttacc gctggccgaa caaggtctgg
    12121 cgtggttaat gccaacagtg gtgatggtgg ttctggccat tatctgggat cgcgcggcag
    12181 gtcgtcaggt gacctccagc gctcactaaa tcactgaaca tttgttttaa ccacggggct
    12241 gcgatgcccc gtggtttttt gtgcacagaa aacccccagc taggctgggg gttccggaaa
    12301 gctttcagct ttgagccagt tattaaaacc ccttttgatt tgttaaaaca ccttgcggtc
    12361 tggcaactgc aagtgt
//
LOCUS       DACBVI010000132        12036 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.133, whole genome shotgun sequence.
ACCESSION   DACBVI010000132 DACBVI010000000
VERSION     DACBVI010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.133"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            54..620
                     /gene="slp"
                     /locus_tag="IU325_17745"
     CDS             54..620
                     /gene="slp"
                     /locus_tag="IU325_17745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HAP5810845.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            773..1303
                     /locus_tag="IU325_17750"
     CDS             773..1303
                     /locus_tag="IU325_17750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP5810846.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQHAFVI
                     IDTLMNNVFHSNLLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEADVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(1364..2392)
                     /gene="chuS"
                     /locus_tag="IU325_17755"
     CDS             complement(1364..2392)
                     /gene="chuS"
                     /locus_tag="IU325_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HAP5810847.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNISEAELAFARVTHDAWR
                     MRGDIRDILAALESVGETKCICRNEYAVHEQVGAFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTTRSERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFIADEN
                     TPLELKAGDAPVVQMRADASVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAETWVTRKPASDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVTA"
     gene            complement(2441..4423)
                     /locus_tag="IU325_17760"
     CDS             complement(2441..4423)
                     /locus_tag="IU325_17760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HAP5810848.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSASDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASDSSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
     gene            5106..6020
                     /locus_tag="IU325_17765"
     CDS             5106..6020
                     /locus_tag="IU325_17765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP5810849.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWA
                     FSVTAAERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGEALVTQINQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATYQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            6040..7377
                     /locus_tag="IU325_17770"
     CDS             6040..7377
                     /locus_tag="IU325_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HAP5810850.1"
                     /translation="MNANNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMLNQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIINILREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGVETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIVREKP"
     gene            7390..7884
                     /gene="hutX"
                     /locus_tag="IU325_17775"
     CDS             7390..7884
                     /gene="hutX"
                     /locus_tag="IU325_17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HAP5810851.1"
                     /translation="MSHVSLQEFLKTEPDGTLEVVAEQYNTTLLEVVKNLPSSTIVSG
                     DKFDTVWDTVCEWGNVTTLVHTADVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHALAASL
                     KEHA"
     gene            7884..8507
                     /gene="chuY"
                     /locus_tag="IU325_17780"
     CDS             7884..8507
                     /gene="chuY"
                     /locus_tag="IU325_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HAP5810852.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVVRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGQDALIISTMGGTQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVHRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            8592..9548
                     /locus_tag="IU325_17785"
     CDS             8592..9548
                     /locus_tag="IU325_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP5810853.1"
                     /translation="MGLSLLLLALVLFGASQGALRISFDALFDEEYRDIWLNIRLPRV
                     LLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIVLPFSFPVVLVLY
                     EQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAAIGILSYIGDEQQ
                     LRQLTLWMMGNLGQAQWPTLLVASSFILPAIMATTCLAGTLNLLQLGDEEAHYLGVNV
                     KRKRQQLLLISSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRWLIPCSALAGACL
                     LLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            9545..10315
                     /locus_tag="IU325_17790"
     CDS             9545..10315
                     /locus_tag="IU325_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HAP5810854.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCVLHDLNLAARYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPASHSPLIVLDH"
     gene            complement(10367..11014)
                     /locus_tag="IU325_17795"
     CDS             complement(10367..11014)
                     /locus_tag="IU325_17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP5810855.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLNHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(11078..11404)
                     /gene="hdeB"
                     /locus_tag="IU325_17800"
     CDS             complement(11078..11404)
                     /gene="hdeB"
                     /locus_tag="IU325_17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HAP5810856.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            complement(11520..11852)
                     /gene="hdeA"
                     /locus_tag="IU325_17805"
     CDS             complement(11520..11852)
                     /gene="hdeA"
                     /locus_tag="IU325_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HAP5810857.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
BASE COUNT         3093 a         2976 c         2830 g         3137 t
ORIGIN      
        1 actataaaga aatatctatt atggttttaa tatttgttga taaggatagt aacatgaaca
       61 tgacaaaagg tgcactcatc ctcagccttt catttttgct tgccgcatgt agttcaattc
      121 cgcaaaatat caaaggcaat aaccaacctg atattcaaaa aagttttgtt gctgttcata
      181 accagccggg gttatatgtt ggtcaacaag cgcgctttgg tgggaaggtt atcaacgtta
      241 tcaatggcaa aacggatacg ttgttagaaa tcgctgtatt accgttggat agctatgcga
      301 agcctgatat tgaagccaac tatcagggcc gactgctcgc cagacaaagc ggcttccttg
      361 atccagtgaa ctatcgtaat cactttgtta ccatcctcgg taccattcag ggtgaacaac
      421 ctggctttat caataaagtc ccgtataact tcctcgaagt gaatatgcag ggcatccagg
      481 tgtggcattt gagagaagtg gttaatacca cctataacct gtgggattac ggctatggtg
      541 cattctggcc ggaaccgggc tggggcgcgc cttactacac caatgcggtg agtcaggtaa
      601 cacctgagct ggtcaaataa caccaccgag agatgcagat gctttctcag catctgcatc
      661 atgcattaca tcaaattaat acacagccag ctaactatta ttataagccc tgtcctgtta
      721 attacctttg gcaaactgat tataaagtta atgtccgcac caggagtcgg ttatgtttct
      781 tataattacc agggatacga tgttcttcac tgcgatgaaa aacattctga gtaaaggtaa
      841 tgtcgttcat atacagaacg aagaagagat cgacgtaatg ttgcatcagc atgccttcgt
      901 cattattgat acattaatga ataatgtatt tcattctaat ttgctcactc aaattgaacg
      961 attaaaacct gtccatgtga ttattttctc cccctttaat attaaacgct gcctggggaa
     1021 agtaccggtg acctttgttc cgcgaactat caccatcatc gactttgtcg cactcatcaa
     1081 tggcagttac tgctctgtgc ctgaagcgga tgtgtccctt tcgcgcaagc agcatcaggt
     1141 tctgagctgc attgcgaatc aaatgacaac ggaagatatt ctggagaaac tgaaaatatc
     1201 gctaaaaacg ttctactgcc ataaacacaa tatcatgatg atcctcaatc ttaagcggat
     1261 caatgagttg gtacgccatc agcatattga ttatctggtg tgatcgattg attaatataa
     1321 agaggcccag caataaatag ccagacctcc cgttaattat acgttatgca gtaacgcctt
     1381 ccggtatcag cgaagcaatt tgcttacgcc attgcgcttg ctcctgttcg ccttctgtac
     1441 gttgaccata aagttgcgct atctgcgtac catcatgggc aaacaattcc agactggtca
     1501 cataaccgtc actagcaggt ttacgggtta cccaggtttc agcaatgctc tcttctaata
     1561 gatgaagagt aaacgtcggg ttaaaaatat tcagccaacc tttcattggc accacttttt
     1621 ctaccacgcc agtgaaaatc tgtacgcagc cacggttgcc aacaaacacc atgatttcat
     1681 tgccatcctg ctgtgcagat tcaagaattt gcgccaacgc actgttggat actttgcagg
     1741 ccaaatcgtc tgccaccaga ttgaacgcct gttggcgcgt caggttgtgg cgcttgagca
     1801 acgtaaaaaa ctgatgaacg tcggtcatcg cccgccactc ttgctcaacc acactggcat
     1861 cggctcgcat ttgaacgact ggcgcatcac cggcttttaa ctcaagcggc gtattctcat
     1921 cggcgataaa ccgtgccaga agctcactcc atgccgccat atcggtattg tcggtggcat
     1981 aaacttttag taatgcatcg ccctgatgat caaagaactg aatactctgg cgttcgctac
     2041 gagtcgtatt ttctttaatg tgaaaaacac tggcccattg attgagaaac agacgcaaat
     2101 ccagcgcgcg tggattgagg atcaatccgg catgtccgtt caaatgctgg tttgtgaacg
     2161 caccaacttg ctcatggact gcatattcat tacgacaaat acatttggtc tcgccaacac
     2221 tttcgagcgc cgccagaatg tcacggatat cgccgcgcat ccgccacgca tcgtgcgtga
     2281 cgcgtgcaaa tgccagttct gcttcgctaa tattcattaa acctgcgatg tcacgcgcgt
     2341 actttccggg attttgttct tttaactcaa gccagcgtgt gtagtggttc attgtctctc
     2401 ttccttccag ataaatgccc cgaaatatcg gggcagatga ttaccattga taactcacga
     2461 aaatttttcc attacgacca tcctgtggga tgccttgtgg cgaccagtac tctttgtcga
     2521 aagcgttacc caacaccaaa gtagtggtca tacctttgag cgcctgttgt ccttgataac
     2581 tgacgtagaa atcattcacg ccatagcctg gttgtttgct gtaactgctg ctgatatgtg
     2641 ttgagcgatc ggcaaacgta ccaacccacc caacagagaa gccactgtga gcgatcggaa
     2701 tattcagggt gctggtaacg gtatccgggt taatactgga gatatattcg ccggtatcgg
     2761 tgtctttgcc gcgggtacgg ttataggcca catcaaggct aaacagatca gtggtatatt
     2821 tcgtcatcac atcccagccc cagattttgg cgttcgggac gttatacgac atagttgtcg
     2881 ccgccgcgaa atcgacggtc gtggagatgt aatccttcgc tttggtatca aagtagctgg
     2941 ctttaaattc cagggcatca ttggacaaca tcaggtcatc aaaacgcagc ccaaaaccgt
     3001 actcctgagt ttcgttagtt tccggacgta agttcgggtt tggcacccaa tagttggtat
     3061 agaagcgacc aatcgagaag tgcttagaat cgttatacat ttcgcccatc gtcggggcgc
     3121 ggaatgcctg ggcatatgag ccaaataaca tcagccagtt agtcgggttg atagtcatcc
     3181 ccgcgcgaga tgaccatttg tcggcatcaa catctttgta gccgtcgctg ctaccgcgat
     3241 aactgtcata gcgggttccg ccaagcagag taatcggcag atctcgtaag gtgatctcat
     3301 cctgtagcca gccggagcta aaatcgattt ttgcttgcgg gaagcccgtc gtcgcgccgc
     3361 ccggatgttg ttcctgacga taatactcac cgccatatgt cagtaagtga gaagcgaaac
     3421 tgtcggcaaa tagagtggaa cggttctcca gcctggctcc ttttgttatc tgttcacgat
     3481 actcgccgga actccccgtg ttttgcgcat taatacggac ttccgaccag taaatttttg
     3541 catctgcatt taaccagtcg ttgccctgcg gggcgagttt ataagaaagc tgcgcatcgc
     3601 gttgaattgt tgaacgatca accatcgggt tgctgctatc agaagcttca acggtctgcg
     3661 gattttttgg ttcacgcgcg tcgttgttgt agtaacgcac taaaccgctc agagactggg
     3721 ctgaatcaat ttgccaggtc cctttcgcca gcatgttatt aatggactcg tcattcggcg
     3781 cggtttcacc attgctctgg cgtaaatcac cccgatcgcg actggaccag gccacaatac
     3841 catccagatt ttcagttcgc ccaaacgcgc tcgcgcctaa tcccaggcta tggtcccccg
     3901 tgccgccagt accaaagaca cgaaaaccac tgctttgtcc ttcctgcaat aaatcttttg
     3961 catcgaccgt atcgtaggag atcactccac ccagcgcacc actgccatac agtaatgctg
     4021 aaggtccgcg aacaatctca acacgcttga tcagcgctgg atcgagaaaa gtgccattca
     4081 ggtgtccggt atccgttccc tggcgaacac catcgacaag aaccagcacg ccgcgatgat
     4141 catagccacg catatttaca tcctggccgt tggttcgtcc ggtaccatca agagtaattc
     4201 caggaacatg acgcagcaga tcggaggctg aagtagccgt ttgattttca ggagcggaag
     4261 tgtcgatgac gctgaccatc ataggcgctt cgaaggaact acgggcattc cccgttgccg
     4321 taacggtcat ggtttcagta gcaaaagcaa acgttggcaa ggtggcagaa acagccaaag
     4381 ccaacaaact caaacgcaac gaggtaaatt gcggacgtga catagcgatt ctccatgagg
     4441 aaaataaaaa atgctggctg cttgggttaa taccggggtg gctggcgaaa aataaattta
     4501 tttaaaattc agagcgataa tattcgccgg gtgagtttac aggcgtttat atcccatgaa
     4561 attgcctggt atctcggcaa atacgcaaaa gcacgccctc tttgtcaggc aaaacgatat
     4621 gccccatcag gagtgaagag cgctgtgcgt tatcccttgc aaatgctatg gttttgttat
     4681 ccatatggtc gtgttttgtt aagcgcaaac gtgataatga gaattattat cgatcaattt
     4741 tttatgatca agcttttttt aaccgccatt aacacaataa acagggggtt atgaaaccta
     4801 tcccgcgccc tgaaaaccac tttattttta tggttattcc cccatgacct catcactaaa
     4861 ctttgtcgtt aacagtgata atttcctctt cgctttgctc tgcaagcctc gatatctctg
     4921 ggttctcagc tttttcccgc gctgaaattt agtcggtaac aagaacgtta gctgacgaag
     4981 catcttgata aaaatcaaaa ataaatacgc ttgtaacgcc gtgcgtattg aatcgacggt
     5041 tgtatttcat atataactca aatgataatc atgatcattc tcaacttgat aagagttctc
     5101 atattatgcc aaggatcatc accaggccgt ttcttttttc cccactgacg ttatgtattt
     5161 cagccgtggc ctctgccgcg aagaccgcgg taaaacgaaa aaaattgttt acggcagtac
     5221 tggctcttag ctgggctttt agcgtaacgg ctgccgaacg tatcgtggtc gcaggaggat
     5281 cgctgacgga gctaatctac gcgatgggcg ctggcgaacg cgtggttggt gtcgatgaaa
     5341 cgacatctta tccaccagaa accgccaaac tgccgcatat tggctactgg aaacaactca
     5401 gcagcgaagg cattttgtca cttcgcccgg atagcgtaat tacctggcag gatgccgggc
     5461 cacaaattgt gctcgaccaa ttacgagcgc agaaagtcaa cgtcgtcacc ctgccgcgtg
     5521 tgcctgccac ccttgagcag atgtacgcca acattcgcca actggcaaaa acgctacagg
     5581 ttcctgaaca aggcgaggcg ctggtgacac agatcaacca acgcctggag cgagtacagc
     5641 aaaacgtggc ggccaaaaaa gccccggtta aagccatgtt tattctctcc gcaggcggaa
     5701 gtgcgccaca ggttgccggt aaaggcagcg tcgcggacgc cattctttcg cttgccggag
     5761 cagaaaacgt cgcaacctac cagcagtaca aaagctacag tgcagaatca ctgattgcgg
     5821 ccaatccaga agtgattgtc gtaacctcac aaatggtcga tggcgatatt aatcggctac
     5881 gttctattgc cggaattacc cacactgccg catggaaaaa ccagcgcatt atcaccgttg
     5941 atcaaaacct gattctggga atgggtccgc gtattgctga tgtcgttgag tctttacacc
     6001 agcagctttg gccgcaataa atggatttaa atcagacgaa tgaacgcaaa taacaccctg
     6061 gatctcacgc cacattttgc tttagacggt gatcagccat ttaaagacag gcgcgccatg
     6121 atgccgtttc gtggggccat tccggttgcc aaagagcaac tggcacagac ctggcaagag
     6181 atgctcaatc aaacggtatc accacgtaag cgactggttt atctacacat tcctttttgt
     6241 gcaacacact gcacgttttg tggtttttat cagaaccgtt ttaatgaaga tgcgtgtgct
     6301 cattataccg acgccctgat tcgtgaaata gaaatggaag cggacagcgt attgcatcag
     6361 tctgccccca tccacgcggt ctatttcggt ggcggcacgc cttccgcgct ttctgcacac
     6421 gatttagcca gaattatcaa tatattacga gaaaagctgc cactggcccc tgattgtgaa
     6481 atcactatcg aaggccgggt actgaatttt gacgctgagc gaatcgatgc ttgtcttgat
     6541 gcaggagcaa accgcttctc aattggtatc cagtcgttta acagcaaaat ccgcaagaaa
     6601 atggcccgca cctcggatgg cccaaccgcc attgcgttta tggaaagcct ggtcaaacgt
     6661 gaccgtgctg cggtggtctg tgacctgctg tttggcctgc cgggtcagga tgcgcaaacc
     6721 tggggagaag atctggctat tgcccgcgat atcggtctcg acggcgtcga tctctatgcg
     6781 ctcaatgtcc tgcccaatac accgctgggc aaagccgtgg aaaatgggcg tactaccgtg
     6841 ccctctccgg cagaacgtcg cgatctttat ctgcaagggt gtgattttat ggacgatgcc
     6901 ggttggcgct gcatcagtaa cagccactgg ggccgtacca cacgcgaacg caatctctat
     6961 aacctgctga ttaaacaagg tgccgattgt ctggcctttg gttccggagc cggcggttcg
     7021 attaatggtt actcctggat gaacgaacgc aatcttcaga cctggcatga atccgtcgcg
     7081 gcaggcaaaa aaccgctgat gatgatcatg cgtaacgccg aacgtaatgc gcaatggcgt
     7141 cataccttgc agtcaggtgt tgaaacagcg cgtgtaccgc tggatgaact tacgccacat
     7201 gccgaaaaac tcgcgccgtt actggctcaa tggcaccaaa aaggcttaag ccgcgatgcg
     7261 tcaacttgcc tgcggctgac taatgaaggt cgtttctggg caagcaatat tttgcagtct
     7321 cttaatgaac taattcaggt actaaatgcg ccagcgattg tgcgtgaaaa accataacag
     7381 gagtgattta tgagccatgt ctcgttacaa gaatttttga aaacggagcc ggacggtacg
     7441 ctggaggtgg tagccgaaca atacaacacc acactgctgg aagtagtgaa aaacctgccc
     7501 tcctcaacca tagtgtcagg cgataaattc gatactgtct gggataccgt ctgcgaatgg
     7561 ggcaacgtca ccacgttagt acatactgcc gatgttatcc tcgaatttag cggcgaactg
     7621 ccttcgggtt tccaccgcca cggctatttc aacctacgcg gaaagcacgg catgtccggg
     7681 catatcaaag cagaaaactg cacacatatt gccttgatcg aacgtaaatt tatgggcatg
     7741 gacaccgcat ccattctctt tttcaacaaa gaaggcagcg caatgctgaa aatcttcctc
     7801 ggcagggatg accatcgtca gctcctgagc gaacaagtca gcgccttcca tgctctggca
     7861 gcatcgttaa aggaacacgc ctgatgacgc cgtggctact ctttggcgca ggcggaaaag
     7921 gtgtgggtgc cagaacgctt gaactggcgc tggcggaaca acgtccggtc gttgctgtcg
     7981 ttcgtcatgc tgatgccgcc acgaaactgg cgcaacaagg cgtacaggtt tttacaggcg
     8041 acgcctgtga tgccagcgta gtcgcggctg cttgccgcgc agctggccag gatgcgctta
     8101 tcatctcaac aatgggcgga actcaggatt atctggcaca cagaacagtg attgatgagg
     8161 ccgaaaaggc cggcatcagt cgcatgattc tggtgacctc tttgggatgt ggcgatagct
     8221 ggccgttttt atcggagcgg gcaaaggctg cgtttggtca ggcggtacgg gaaaaaacgc
     8281 tggcggaaag ctggttgcaa accagccagc ttgattacgc cattctccgc cctggcggtc
     8341 tgcttgatgg ggcggcaacg ggcaaagcac aacggataca aaatcaggag tgtcatggtt
     8401 ttgttcaccg tgccgatgtc gccgcacata tccatgaact ggcaaatgcg ccagcactta
     8461 accagcaggt ctacagtctg attgaaccgg acctgaaacc ggcgtgatac cccggcgcgg
     8521 catgtccgcg ccgtaatgtc gtaacggagt tttgcgtgct caaggattca ctctcttccg
     8581 ccagggtctt tatggggcta tcactattat tgctagctct ggtgctgttc ggtgccagtc
     8641 agggagcgtt aaggatcagt tttgatgctc tttttgatga ggaataccgt gatatctggc
     8701 tcaatattcg tctaccacgg gttttgctgg cggtgctggt aggtgcagcg ttggcaaccg
     8761 ccggggtaat tatgcagggg ctttttcgca acccaatggc tgacccagga ttacttggcg
     8821 tcagtagcgg ttctgcatta atggttggcg ttgctattgt gctgcccttc tccttcccag
     8881 tggtgctggt gctctatgag caaatggtgt ttgccatcgc cggaagttta gtggtctgta
     8941 ccatcatttt tctcatcacg cagcgccatc gcgatggcag catgatgcaa ttgttactcg
     9001 ccggtatcgc catcaatgcc ctgtgcggcg cagcgatcgg catcctgagc tatatcggcg
     9061 atgagcaaca gctacgacaa ctcacgttgt ggatgatggg caatctcggt caggcgcaat
     9121 ggccaacgtt attggttgcc agttccttca ttctgcccgc cattatggca acaacgtgtc
     9181 tcgccggaac gctgaatttg ctgcaacttg gtgatgaaga agcccattac ctcggcgtga
     9241 acgtaaagcg taaacgccaa caattactgt tgattagctc actgctcgtt ggtgccgccg
     9301 tatcggtaag cggcattatc ggctttattg gcctggtgat cccgcatctg attcgtatga
     9361 ctaccggggc aaatcaccgc tggctaatcc cttgctccgc cctcgccgga gcctgtttat
     9421 tgctgatggc agacacgctt gcccgcacgc tggtacagcc agcagaaatg cccgtgggat
     9481 tattaaccag cctgcttggc ggtccttatt ttatgtggtt gattctgcgc aaccggagga
     9541 tcacatgatc agcgcccaga atctggttta tagccttcag gggcggcgtc tgactgacaa
     9601 tgtctcgctg acttttcccg gtggggaaat tgttgccatt ctcggcccta atggtgcggg
     9661 aaaatctacg ctgcttcgcc agttaaccgg ctatcttcaa cctgatagcg gcgaatgtcg
     9721 gttgtttaat aagccattaa atgaatggtc gattactgaa ctggcgaaac accgggccgt
     9781 gatgcgtcaa aatagccaca tggcgtttcc attcagcgta caagaagtta tccagatggg
     9841 gcggcatccg catcgcactg gtaatcagga taatgaaaca gcacaaataa tggcgttatg
     9901 tgactgtcag gcgttagcaa accgtgacta tcggcaatta tccggtggtg aacaacagcg
     9961 cgtgcaatta gcgcgcctgc tggtgcaact ctgggaacct actccctcac cgaaatggtt
    10021 atttcttgat gaaccaactt cggcgctgga tattcaccat cagcagcact tgtttcgttt
    10081 attacgccag ctagttcacg aacggcagtt caatgtttgc tgcgtattgc acgatctaaa
    10141 tcttgccgcg cgttatgcag accgtgttgt attaatgcaa aaaggaaagg ttatcgcaaa
    10201 tggtaaacca caggacgtat tgacacagca ggcgctgacc atgctttacg gcgcggatat
    10261 taccgtactt aaagatcctg ccagccattc acccctgata gtcctcgatc attaaaacca
    10321 acaggagtac cgctctggta ctcctgtgaa taaataacta taaagattag ctaattgaaa
    10381 cgcctttcac ccctgccata ccttttaata atcgcaacag gtcttctatc gacgttgtgg
    10441 catgaagctg gatatcaatg gcgaccactt catgatcttc attttcctgc aacgaaacta
    10501 aatcagtttt tattttttgc tgcttaaacc agtcgagcat tgataccgta ttaccgttca
    10561 ctaatgtcag ctgtaaatgg taatttttgt tcatcaggcg gaaggttaat tgatggaaga
    10621 cttccagcac cagcaaggtc atcactgaac cataaatgcc cagttcgtac ataccgctac
    10681 caataaccat accaatggcg gcggtcaccc agatatccgc tgccgtcgtc agacctacaa
    10741 tattttggtt acgaacgaga atgttacctg cgccgataaa cccgacgccc gtcaccacct
    10801 gagcagcaat acggctgggg tccagtccga cgtgatttaa agacagcacg tcagcaaaac
    10861 catatttcga aacaatcata aacagggcgc ttcccatgcc aattaataca tgtgtgcgta
    10921 accctgctcc tttgccgcgc atttgccttt ccatgccaat agcgccacag gcaattgctg
    10981 cgagtataag acgtataata aattctgctg tcatataaaa aagcaaaaag gagcagcaag
    11041 atggctcaac ttgctactcc tttttacttg tacctcatta attcggcaag tcattagatg
    11101 cttgattttt gaaggtatac aaatttttct gcgggttttt cttgcagtat tcgatcactt
    11161 taggaatttg agtgagatcg gtttcattta aagtaacggt atcgccacct ttatatactg
    11221 tctcttcatg cagcatccac catgcaaccg gtgtcattgc ttttggattc agatcaataa
    11281 attcctggca ggtcatatct ttagcggatt cattggctgc cagcgcagat tgtgcgttca
    11341 ccagtgacaa agccgctaca gcgcccataa aaataaacgc tttacggaga gatgaaatat
    11401 tcattttgta acccattcaa tatagagatt atatatgaac aggaggaata gctgctttta
    11461 aagaagatat tccggcctgt tcaatatgca aggaagtacg atgtaacgca ttcccggaat
    11521 tacatatctt tcttaatttt gtcccactcg cctttaactt tatctttaaa gttggcttgt
    11581 ttatcctgag tacaagcctg aacgatagct ggggttacgg ttgcaatacc ctgaacatct
    11641 aaaaccgcat cttctggttt atctttgttg ttcagcgctt cagcaaaacc aactgcagtt
    11701 ggctggaagg attcgtccac agccaagaaa tcttcacagg tccaggagtt gaccggtttt
    11761 ttgttatcgg ctgctttttg cgcatccgct gcattgctca caactggcag aagaagcaga
    11821 ccaccaagaa taacgcctaa tacttttttc atcgtaatat cctcaactat aaagtgaaag
    11881 agccgtcacg aatcaatttc gacactgagg ttataacctg gttttctgta tatgtcatgt
    11941 tgatggaaaa tatcaaaatc agatattttt atttcaatac aatgagttac agatgcatca
    12001 gatactgtaa ttaggaaatt tttattaaat cgactg
//
LOCUS       DACBVI010000133        11945 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.134, whole genome shotgun sequence.
ACCESSION   DACBVI010000133 DACBVI010000000
VERSION     DACBVI010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.134"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            344..667
                     /locus_tag="IU325_17810"
     CDS             344..667
                     /locus_tag="IU325_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP5810858.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGDQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            664..1494
                     /locus_tag="IU325_17815"
     CDS             664..1494
                     /locus_tag="IU325_17815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="catalyzes the cleavage of the bond between muamic
                     acid and l-alanine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP5810859.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(1491..2504)
                     /locus_tag="IU325_17820"
     CDS             complement(1491..2504)
                     /locus_tag="IU325_17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP5810860.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPSLTHYGVSKLNFDFTLDITRAQEELGYQPVLTLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(2603..4033)
                     /locus_tag="IU325_17825"
     CDS             complement(2603..4033)
                     /locus_tag="IU325_17825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HAP5810861.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPACEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTEL
                     RALIPQRLIAFDDAVRRTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKRIARLAEQSTD"
     gene            complement(4044..5045)
                     /gene="ltaE"
                     /locus_tag="IU325_17830"
     CDS             complement(4044..5045)
                     /gene="ltaE"
                     /locus_tag="IU325_17830"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HAP5810862.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(5082..6800)
                     /gene="poxB"
                     /locus_tag="IU325_17835"
     CDS             complement(5082..6800)
                     /gene="poxB"
                     /locus_tag="IU325_17835"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HAP5810863.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATSHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(6933..7901)
                     /locus_tag="IU325_17840"
     CDS             complement(6933..7901)
                     /locus_tag="IU325_17840"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HAP5810864.1"
                     /translation="MTMPTNQCPWRMQVHHIKQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRSPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLTRVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(7913..9565)
                     /gene="hcp"
                     /locus_tag="IU325_17845"
                     /gene_synonym="priS"
     CDS             complement(7913..9565)
                     /gene="hcp"
                     /locus_tag="IU325_17845"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HAP5810865.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(9709..10608)
                     /gene="lysO"
                     /locus_tag="IU325_17850"
     CDS             complement(9709..10608)
                     /gene="lysO"
                     /locus_tag="IU325_17850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HAP5810866.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITIILLCNIIALLWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(11092..11787)
                     /gene="aqpZ"
                     /locus_tag="IU325_17855"
     CDS             complement(11092..11787)
                     /gene="aqpZ"
                     /locus_tag="IU325_17855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HAP5810867.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGDYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
BASE COUNT         2924 a         3199 c         3173 g         2649 t
ORIGIN      
        1 tattgagatc ttattacttt cttgttttgc ggcggctgac caaaaagcaa tccagagata
       61 tatacctcag gcgcaagatg gggaagactt taggaatcat ttacaatata agttgactct
      121 tcccactcat taatgttgct ggtactacat aatacgacag gtaagcggtg ctccataata
      181 aaactaatat cgggttatgc aacggataat tgtacaggct tatgtacctg tttttgacac
      241 aatgagatga tttcctaaac tgatcaaaac cttcttccca acatggcata tcagtccgat
      301 aatgctttag agatcgaagg cttaacaaac aaggagatcg atcatgcaat tttctacaac
      361 cccaactctg gaaggccaga ccatcgtcga atattgcggt gtggtgaccg gcgaagcgat
      421 tttaggtgcc aatattttcc gtgatttctt tgccggtatc cgcgatatcg ttggcggacg
      481 ctccggggct tatgaaaaag aattgcgtaa agcgcgggag attgcctttg aggagctcgg
      541 cgatcaggcc cgggcgctgg gggctgatgc cgtagtcggt attgatatcg actacgaaac
      601 ggtcgggcaa aatggcagta tgctgatggt tagcgtcagc ggtacggcgg tgaaaacgcg
      661 tcgatgagaa gagtcttctg gctggtcgcc gccgctctgt tactggcagg gtgtgccggc
      721 gaaaagggca ttgtcgagaa agagggatat cagcttgata cccgacgcca ggcgcaggcg
      781 gcgtatccgc gcattaaagt gctggttatc cactacaccg cagatgattt tgatagctcg
      841 ctggcgacac tgaccgataa gcaggtcagt tcgcattatc tggtgcctgc tgtgccaccg
      901 cgatacaacg gtaaaccgcg catctggcaa ctggtgccgg aacaggagct ggcctggcat
      961 gcggggatta gcgcctggcg cggggcaacg cgccttaacg acacctctat tggcattgag
     1021 ctggaaaacc gtggctggca gaaatcatcc ggagtgaaat attttgcccc gtttgaacag
     1081 gcacagattc aggcgcttat tccgctggcg aaagatatta ttgcccgtta tcacatcaag
     1141 ccggaaaacg tagtggcgca tgcggatatt gcgccgcagc gcaaagacga tccggggccg
     1201 ttattcccct ggcaacaact ggcgcagcag gggattggtg cctggccgga tgcgcaacgg
     1261 gtgaactttt accttgccgg gcgcgcgccg cacacaccgg tagataccgc atcattgctg
     1321 gagcttttgg cgcgctacgg ttatgacgtt aaacctgata tgacaccgcg cgagcagcgg
     1381 cgcgtgatta tggcgttcca gatgcatttc cgcccgacgt tatacaacgg cgaagcggat
     1441 gcagaaactc aggcgattgc cgaagcgttg ctggagaaat acgggcagga ttagcgcggc
     1501 agttttccgt ggtcgcgtag ccaggcggca gttttctcga taccttcatc cagggtgagg
     1561 actggttgat aacctaactc ttcctgcgca cgcgtaatat ccagcgtaaa gtcaaaattc
     1621 aacttggaga cgccgtagtg ggtcagcgac ggctcttttg ctgacttgcg gcctaaacgc
     1681 tccatgctgc gggcgatcat atccagcatc gggtagggga cggaacgaat acgacagtca
     1741 atattcaact cgtcgatcag cttctgcacg atgctgcgca gtgtgcgatg ctcgccgttg
     1801 gtgatgttgt acacacgccc ggaaggtagc ttatcgcagg cttcctggct tgccagccac
     1861 atggcgtgca cggcattttc atagtaggtc atatcgacca gcgcactgcc gccgtgcggt
     1921 aacagaatgc tgccgtagtg gtgcatcata tgcgccagac gggggataaa gactttatcg
     1981 tgcggtccga acagactttg tgggcgcaga atagtaaagc gcgtttgtgg attcgcctgc
     2041 gaaagcatat tgatcacttc ttcgctggct gctttgctgc gggcaaactc gttggcaaag
     2101 cggtgagggc gaaaatcttc tttaatatcg cgatggtggt gataatcgaa gtacagggag
     2161 ggggaagaga tatgaataaa gttacgcaca ccccaggcga cagcccattc acccaggcgg
     2221 cgagtggcgc gaacgttagc cagatcgaaa gcctgttgtg tcccccaggg cgaggtaaag
     2281 ctggagcagt gccacagcgt atcaatgccc gcgagcatca ctttagcttg tgatgagacc
     2341 agctcggtca gatccgccgg aacaaactct gcgcccattt tttccagcaa tttacccatt
     2401 gcctcgttgc gaccggtcgc tcgcacgctg atgcctttct ggcataaaaa ctctaccgcg
     2461 tttcgaccta agccgctggt ggcgccggta accagtacct tcatatcaat ccactgttgt
     2521 tgagaaaatg acgtgcgcat tcttccgtga tttcccccat gatgcaatgg gaaacatgaa
     2581 agaataacgc aggttttgtc gattaatctg tgctttgttc tgccagtctg gcgattcgtt
     2641 ttgccattcc gcgaaaaata aacagatgcg cggggatcat caataaccag taaaacagcc
     2701 ccggcatacc gtgcggatgc cagaatgcgc gaacatcgat agtacgatag tcgcctttat
     2761 cttccagggt aaaacacagt cgtcccagcc ccggcgcttt catgccaaat aacagcgtaa
     2821 gttgtttttc cggttcaacg acaatcactt tccagctatc caccgcatcg ccggtctgca
     2881 aatattcacg ctccgggcgg cctttcgcca gcttatgacc gatcgcgcgg tccatcaacg
     2941 cccgggtttg ccacaaaata ttgccaaaga aataacgctc tttaccgccg atttgattca
     3001 ccacctgcca taaagccgca aggctggcgg acgttttaac ggtaaacccc gcctgtttgg
     3061 cgaaataacc gtactccggt cgccagcggg caaaggcctg agcgtcgtag ccccagtcgc
     3121 tggagttgac cagcttttct tcctctttca gggtgcgacg taccgcgtca tcgaaagcga
     3181 tcagccgttg tgggatgagc gcacgtagct cggtgtcatc cgccagcaga tcgtgtttca
     3241 gcccctgaat caacgccctg gcggtggtgg gtggtacgga agtaatcaca ttgagaaacc
     3301 acaccgaaat ccagcgggtg gggagaggga tggggatcaa ccagcgacgc ttaccgctca
     3361 ccgccataaa atgttcaaac tgttgctgat aactgagcac ttctggtccg gcggcttcga
     3421 agatgcgatg ttcgcaggcc ggatgatcta acagcgccac cagatagtgc agcaagtttt
     3481 ccagcgcgat gggcgtggtg cgtgaacgta cccagcgtgg cggcgttaag actggcaggt
     3541 tgtagaccat atcgcgcatg acttcgaacg ccgctgaacc tgcaccaacg attattccgg
     3601 cacgcaattc ggtcaccggg acacccgctt cacgaagaat atccgccgta gcctgacggg
     3661 cacgcagatg gtccgattgc tcatgtggcg gggcctgcaa cgaactgaga aagattaatt
     3721 gcttaactgg gacttcacgt agcgcatcgc ggacgttgag agccacctgg cgctcctgag
     3781 cgataaaatc gccgccttcg cccatgctgt gcaccagaaa atagaccgta tcgatatcct
     3841 gcaacagggc cggaaggtta tccggccagc tgagatcgac tttatggcaa ctgacatttg
     3901 ccagttgcag ctttgcaagc ctgtcgacat gacgtgccgc cgccaggatc tgatgcccct
     3961 gctggctgag tgttcgcacc agatgctgac caatgtagcc actggcaccg agaactaaaa
     4021 tgcgttgcgg cacgtctccc tccttaacgc gccaggaatg cgcgccagtg ggcggcgact
     4081 tcagccagtt gctcgcgcga gacgtcgaga tgcgtcacca ggcggacaat cggcgaggcg
     4141 ttaatcagca cgtttctcgc tttcatgtat tcgcctaacg cggcagcatt ttcttccccg
     4201 acgcgaacaa acagcatatt ggtgtcctga cgcatcacat ccgcgcctgc ttcacgcaac
     4261 tgctccgcca tccaggcggc gttgtcgtgg tcttcctgta agcgcgcgac gttatttttc
     4321 agggcataca tcccggcggc agccagaatg ccggactggc gcatcccgcc accggtcatt
     4381 ttccgccagc ggatggcacg tttaatgtaa tcacgattac cgaccagtaa cgagccgact
     4441 ggcgtcccaa gtcctttcga caggcaaatg gtgaacgaat cacaatattg cgtgatctct
     4501 ttcagttcgc agccgtaagc caccacggca ttaaagatgc gcgcaccgtc aacatgcagc
     4561 gccagattgc gctcgcgagt aaattcccat gcttctttca ggtattcccg cggcagcact
     4621 ttgccgttgt gggtgttttc cagactgagt aatttggtgc gggcgaaatg gatatcgtcg
     4681 ggtttgattt tcatcgccac tttatccagc ggtagcgtgc cgtcggcagc cgcgtcgatg
     4741 ggttgcggtt gaatactgcc cagcaccgcc gcgccaccgg cttcaaacag atagttatgc
     4801 gcggcctgac cgacaatata ctcttcgccg cgttcacagt gactgagcag agcgaccagg
     4861 ttggcctgag tgccggtagg cagaaaaatg gcggcttctt taccggaaag ctctgctgca
     4921 tagtcctgca gagcattaac ggtagggtcg tctccgtaaa cgtcgtcccc aaccggggcg
     4981 gccatcatcg cttcgagcat ggcgcggctc ggtcgggtaa cggtatcact gcgtaaatca
     5041 atcatggcat gtccttatta tgacgggaaa tgccaccctt tttaccttag ccagtttgtt
     5101 ttcgccagtt cgatcacttc atcaccgcgt ccgctgatga ttgcgcgcaa catatacagg
     5161 ctaaaacctt tggcctgttc gagtttgatc tgcggtggaa tggctaattc ttctttggcg
     5221 accaccacat ccaccagcac cggaccgtcg atggaaaagg cgcgttgcag ggcttcatcg
     5281 acttcagagg ctttttccac gcgaataccc gtaattccgc acgcttcggc aatgcgggca
     5341 aagttggtgt catgtagttc ggtgccgtca gtcaaatagc caccagcttt catctccatc
     5401 gccacaaagc ccagcacgct gttattaaag acgataattt tcaccggcag tttcatctgc
     5461 actactgaga ggaaatcgcc catcaacatg ctaaaaccgc catcgccgca catggcgacc
     5521 acctgacgtt ccggctcggt tgcctgagca cccagcgcct gcggcatggc gttagccatc
     5581 gaaccgtggt taaacgaacc taagagacga cgcttgccgt tcattttcag ataacgtgcc
     5641 gcccacaccg ttggcgtacc aacgtcacag gtaaagatgg cgtcatcggc ggcaaaatga
     5701 ctaatttgct gcgccagata ttgcgggtga atggctttct cgctcggttt agctaaatcg
     5761 tccagccctt tgcgggcgtc gcggtaatct tccagcgctt tatccagaaa cttgcgatcg
     5821 gctttttctt ccaccaatgg cagcaatgca cgcagagttg acttgatatc gccgaccagt
     5881 gccatatcca ccttgctgtg agcgccgatg ctggctgggt tgatatcaat ctgaatgatt
     5941 ttggcatcgg tcgggtagaa ggcgcggtag gggaactgag taccgagtag cactaacgtg
     6001 tcggcgttca tcatggtatg gaaacctgac gagaagccaa ttaatcccgt cattccgaca
     6061 tcatacggat tatcgtattc gacatgttct ttaccgcgca gggcatgaac aataggcgct
     6121 ttaattttcc cggcaaactc aactaactct ttatgcgccc ccgcgcagcc gctgccacac
     6181 atcagagcga tattgctgga ataacgcagc agttgcgcca gtttgcgtaa ctcttcttct
     6241 tccggcgtca cgactggctg tggcgcatga taccagtgcg aggttgcccc ttctggcgca
     6301 ggttttaacg ccacgtcgcc cggtaacacg acaacggaaa cgccacggtt tagcaccgct
     6361 ttgcgcatgg caatcgccag cacttgtggg atttgctccg ggctggaaac cagctcgcaa
     6421 tagtgactac attcgcggaa taactcttgt gggtgggttt cctggaaata gccgctgcca
     6481 atttcgctgg agggaatatg agcggcaatc gccagtaccg gaacgtgatt gcggtggcaa
     6541 tcgaacaggc cgttgattaa gtgcaggttg ccggggccgc acgatccggc acagaccgcc
     6601 agttctcctg aaagttgtgc ttcagcacca gcggcaaagg ccgccacttc ttcatggcgg
     6661 gtggacatcc actcgatggt gcccatgcga ttaagactgt cgctaagacc gttcagagag
     6721 tcgcccgtga ctccccagat gcgtttcacg cctgccgatt cgagtgtttt ggcgatataa
     6781 gctgcaaccg tttgtttcat ggttctccat ctcctgaatg tgataacggt tacaagttta
     6841 gttcatctga cggaggggga agggatggga gaaaaaggag gcactaacgg ttaaatagcc
     6901 cgatgaaagg aatatcatcg ggcataaggc gattatgcga gaaccaaatc tccctgcgga
     6961 tggcaggagc aggccagcac gtaaccttca gcgatttcgg cgtcggtcag cgtcattgtg
     7021 ctgctcaccg tatattcacc ggaaaccact ttcgtcttac agcagccgca aacacccgca
     7081 cggcaggcag cgacaaccgg aacgttattg ctttccagcg cctccagtag cgtggtgcca
     7141 accggggcgt aaaattctcg tgccggttgc agtttggtga atttcagacc gctggtcgcc
     7201 gcttccgcta ctggggtgaa gaatttctct ttaaagaaac gcgtcacgcc gagcgctttc
     7261 acttcctgct ctacccaatc catatacgga gccgggccgc aggtcatcac ggtacgtgaa
     7321 gctaagtcag gaacgcgagt cagcagttcg cgagtgagac gaccagcgat aaagccttcg
     7381 gtaacgttat tttctgccac cagcgtcacc ggatagttac gccactcatc ggcgaaaata
     7441 acatcctgcg gcgaacgcac gttgtagatc acctgcacat cggcctgcgg acggttcttc
     7501 gccagccagc gacgcatcga cataatcggc gtgacgccgc agcctgccgc cagcaacagg
     7561 aatttatctt ctgctttatc gtcgcaggta aattccccca tcgcgtccga aagccagaga
     7621 taatcaccgc gttttacatc gcgcgtcagc cactgggagc cgacaccgtc atcaatccgc
     7681 cgcacagtca gggtgatgta ttcactcacg cctggcgtgg aggaaatggt gtaagcacgc
     7741 agcgtttcgg ctgagttacg cacgctgacc agtgcatatt gcccggcgcg atatgggtag
     7801 taatcgtggc aaatcaggga aatcgtccac acatccggcg tttcttgctt aatgtgatga
     7861 acctgcatcc gccacgggca ttgattcgtt ggcatcgtca tcgacaaact ccttacgcgc
     7921 tcaacagttg cttcatgtct tcttcaacag tggtgataga acgcaggccg aatttctcgt
     7981 tcagcaccgc cagcaggtct ggtgtcagga aaccaggtgc agtcgggccg gtgacgatat
     8041 ttttcacacc cagagaaagc agtgtcagca gaatgacaat cgctttctgt tcaaaccacg
     8101 agagcaccag cgacagcggc agatcgttga ctccgcagcc cagtttctct gccagcgtta
     8161 ccgccagaat aatcgctgag taagcatcgt tacactgacc agcatctacc agacgcggca
     8221 gaccttcgat atcgccaaac tcgagtttgt taaagcgata tttaccacag gcgagagtca
     8281 ggatcagaca gtcatccggc acgctggtgg cgaaatcggt gaagtagtga cgctcgccgc
     8341 gtgcgccgtc acagccgcca agcaggaaga tatgacgcag tttttcacga cttaccagat
     8401 caatcagcgt atccgctgcg ccaagcagtg tctggcgacc aaagcccacg gtgatcaggt
     8461 gcggaatttc gctgtacggg aagcccgcca tttgttgcgc ctgggcgatg accgcagaga
     8521 aatcttcgcc atccagatga cgcacacccg gccagccaac gatgctgcgg gtccagatac
     8581 gatcgtcata agcgcctacg gttgggtcga tgatgcagtt cgaggtcatc acgatggggc
     8641 cagggaaacg agcgaactcc acttgctgat tctgccagcc gctgccgtag ttaccgacca
     8701 gatgcttgaa tttacgcagc tccgggtagc catgcgcagg cagcatttcg ccgtgggtgt
     8761 agacattaac gcccgtgcct tcggtctgtt ccagcaggtt gtagagatct ttgagatcgt
     8821 gaccggaaat cagaatgcat ttacctgccg tcgctttgac gttgacctgg gttggggtcg
     8881 ggtgaccgta tttaccggtt tcgcctgcgt ccagaatgct catcactttg aagttcatct
     8941 ggccgatttc cattgaacac tcaagaagcg cgttcatatc ggcaggccag gttcccagcc
     9001 acgccatgat tttatggtac tgggcgtaaa tatcgttgtc gtattgaccg agaacatgcg
     9061 cgtgttccat ataggccgcc gcacctttca ggccatacag gcacagcaga cgcaggccga
     9121 gaatgttttc gccaatcgcc gctttatctt tgttaggggt aaattctgct gcctgacgtt
     9181 gcagctcgcc gagatcatcg ctcaccagtt gcaggtcagc catcgggtta tcgacgcggg
     9241 cgttggcatc tacagccagg cattgtgctt tcagcgcctc gcgcagggca atcgcttcac
     9301 gagcgtagcc gactatacgc ggagaatcga agttaacgtt ggtcagggtt gagaaaaatg
     9361 cgcgtggcgc aaagctgtct acatcgtggt tgatgatgcc gtattcacgc gcttttaccg
     9421 cccaggccga aagcccttgc agcgccgcga tgagtaaatc ctgaaggtca gaggtttccg
     9481 ccgttttacc acacatcccc tgcgcgtatg agcagccgtt tcctgccgga gtacggatag
     9541 tttgttcaca ttgcacacaa aacatgatca caccttttaa agttatattt aatatacatg
     9601 tttaaggtta agacgcttaa cgcagggata aaagggattt ttcatgcaac tttaagggag
     9661 attgatttag cgcaattttg gcggcaggga tctaccgcca gagaggtatt acgcagagaa
     9721 aaaggcgatg aggatcggca ctaacaggct aagaataaaa ccgtgaacaa ttgccgccgg
     9781 gaccatatcc agcccgccag tacgttgaag aacgggcagg gtgaaatcca tcgacgtcgc
     9841 accgcataag cccagtgcgg tagagcggct gcggcgaatc agcccaggga tcaacataat
     9901 tgcaatcagc tcacgggcca gatcattaaa aaacgccgcg ctcccgatta ccggaccaaa
     9961 agattcagtc aataaaatac cggaaagaga ataccagccg aaaccagagg ccattgccag
    10021 cgcggtattg atgggaagat caagaataaa ggcgttaatt aaaccaccaa ttaatgaact
    10081 ggcaaccacc accacggcga caatcattcc ccggcgatta aggacaatct gctttaaggt
    10141 catgccatta ttgcgcaact gaataccgac gaggaaaagt agcagaatta aggtgtattc
    10201 actggcttcg gtcgcatgtt gtaagaaagc cagtccactc aggccaatgg caaaaccaat
    10261 cactactacg ccgcacagtt ttagcgactc cagcgccatc gcaatacgcg acgggagttt
    10321 ttcttgctgg tggtggttgc gccacggcag accgcgctcc agccacaaca acgcaataat
    10381 attgcagagt aaaataatgg taatgctgac ggcggaataa tgcagaatcg ccaacaggtt
    10441 actggcgagg ttatcgagaa acgccagact gatacccata aaaaagagaa tgaggtaaac
    10501 catccagctt aatagctgat taataactct taatgcagct ttttggcgaa gaggaatgag
    10561 gtaacccaca atcaggggaa ccagaatgat taacagccca gaaaacatga aaacccagtc
    10621 cttgcaaaga tgaagtcgaa atgcgcgatg acacactact gaaagcggaa ggacgagtaa
    10681 agttgcaatt aaaaggaaat gttatgcata aggatcggta gggtattcat tttcatttaa
    10741 agatattctt gcgctttaat tacaaaccgc accggtgttg gtggcgtcaa aatcgccgag
    10801 gcgttccctg aaggccgggg cagcccacat ggatgtgggc tgagggcgcg ttttacaggg
    10861 atgttacctc gcgcccgacc cggtagccgt aagggataag tcgagggcac cgcgcagcgg
    10921 cgattttgtt cgccagagcc cgggggtgca gggggcggcg gcgattggcc gccccctgcg
    10981 cgctccctgc gccagtgact atatgtggct taacacatca aggagcgcaa caggaagatg
    11041 aatcaatata tttaaaaaag gcctgacatt gcgccaggcc ttctgcgtta attaattacg
    11101 cttttccagt agagtccggt aaatcagacc accgataata ccgccgacaa ttggcaccac
    11161 ccagaagaac cacagttgtt ctaacgccca gccgccctgg aagatagcaa ccgcggtgct
    11221 gcgcgccggg ttaacagaag tgttagtcac cggaatactg attaagtgaa tcagggttaa
    11281 ggccagacca atagcgatcg gcgcaaaacc agcaggagcg aatttgtcgg ttgcgccgtg
    11341 gatcaccagc aggaaacctg cactcaatac cagttcaact accagcgcgg aaagcatgga
    11401 ataatcgcct ggcgaatgct cgccataacc gttagaagca aaaccgctgg ctgccgcgtc
    11461 aaaacccgtt ttaccactgg caattaaata cagcagcgcc gccgcaacaa taccgccgac
    11521 aacctgggca attacgtagc caacgacttc ttttgccggg aaacgtccgc cagcccataa
    11581 accaatagtg accgccgggt taaaatgacc accagaaata tgaccaacag caaaggccat
    11641 cgtcagaacg gtcagaccga atgccagcgc cacgccggca aaaccaatgc ctaattccgg
    11701 gaagcctgcg gccagtacgg cactaccaca gccaccaaaa acaagccaga aagtaccaaa
    11761 acattcagct gctaattttc tgaacatatc cacctcaatt aaaaattgac cctgtgaaaa
    11821 atatggtcgt tttatagggt cgtcgtaaaa aagtgacgac ggaaataatg cgcggctatt
    11881 ttaaaaacga aggcgagtca ttcaccagat aaataaatcc agtaaatttg atttagggca
    11941 acagc
//
LOCUS       DACBVI010000134        11732 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.135, whole genome shotgun sequence.
ACCESSION   DACBVI010000134 DACBVI010000000
VERSION     DACBVI010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.135"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            8..610
                     /locus_tag="IU325_17860"
     CDS             8..610
                     /locus_tag="IU325_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HAP5810868.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(612..1133)
                     /gene="ruvC"
                     /locus_tag="IU325_17865"
     CDS             complement(612..1133)
                     /gene="ruvC"
                     /locus_tag="IU325_17865"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HAP5810869.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFEPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1168..1908)
                     /locus_tag="IU325_17870"
     CDS             complement(1168..1908)
                     /locus_tag="IU325_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP5810870.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1937..2389)
                     /gene="nudB"
                     /locus_tag="IU325_17875"
     CDS             complement(1937..2389)
                     /gene="nudB"
                     /locus_tag="IU325_17875"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HAP5810871.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2507..4279)
                     /gene="aspS"
                     /locus_tag="IU325_17880"
     CDS             complement(2507..4279)
                     /gene="aspS"
                     /locus_tag="IU325_17880"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP5810872.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANNPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4589..5155
                     /locus_tag="IU325_17885"
     CDS             4589..5155
                     /locus_tag="IU325_17885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP5810873.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAVKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILNAL"
     gene            5152..5970
                     /locus_tag="IU325_17890"
     CDS             5152..5970
                     /locus_tag="IU325_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP5810874.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKS
                     EVVLRWREQTTDNFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRDLPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHTVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            6023..6418
                     /locus_tag="IU325_17895"
     CDS             6023..6418
                     /locus_tag="IU325_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810875.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6459..7202
                     /gene="cmoA"
                     /locus_tag="IU325_17900"
     CDS             6459..7202
                     /gene="cmoA"
                     /locus_tag="IU325_17900"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HAP5810876.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HQAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7199..8170
                     /gene="cmoB"
                     /locus_tag="IU325_17905"
     CDS             7199..8170
                     /gene="cmoB"
                     /locus_tag="IU325_17905"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HAP5810877.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(8205..10634)
                     /locus_tag="IU325_17910"
     CDS             complement(8205..10634)
                     /locus_tag="IU325_17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAP5810878.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAVDQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLAGKSDNTPKSAAWAAEISGVPEAQ
                     IVKLAELMAANRTILMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAAQFEARNDFDVFADLAELLKPGGKEIYTEGRDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGSADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNRRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(10659..11732)
                     /locus_tag="IU325_17915"
     CDS             complement(10659..11732)
                     /locus_tag="IU325_17915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020237714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HAP5810879.1"
                     /translation="MFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHSMSKPFEEYQ
                     GTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDSDDKFEAHRQ
                     EMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGETCIDCHKGIA
                     HFPPEIKMDDNAAHELESQAATSVTNGVHIYPFKTSRIGELATVNPGTDLIVVDASGK
                     QPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADEYGNQWRQAS
                     LQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIASDHYTVNAWPSIAKGMGARTSM
                     SENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2667 a         3124 c         2877 g         3064 t
ORIGIN      
        1 tgacaacgtg aatattaatt accctgctga atatgaaatt ggagatatcg tctttacatg
       61 tataggtgct gccttatttg gtcaaatatc agctgcatca aattgctgga gtaatcacgt
      121 cgggatcatt atcggtcata acggtgaaga cttcctggtt gcagaaagcc gggttcccct
      181 ttcaaccatc accacgctat ctcgttttat taaacgttcg tctaatcaac gctatgctat
      241 aaagcgatta gacgctgggc tgacagaaca acaaaaacaa cgaattgttg aacaggttcc
      301 ctcccggcta cgcaaacttt accacaccgg ttttaaatac gaatcttcgc gccagttctg
      361 ttcaaaattt gtttttgata tttataaaga ggcgctatgt attccggtgg gtgaaataga
      421 gacgtttgga gaattgttaa atagcaatcc gaatgcaaaa ctcactttct ggaaattctg
      481 gttcctgggt tctattccgt gggagcgtaa aaccgtcacg ccagccagtt tgtggcatca
      541 tccgggtttg gtgttgattc atgcggaggg agtggaaacg cctcagccgg aactgaccga
      601 ggcggtataa cttaacgcag tcgccctctc gccaggttca gccgcgattc gctcatctgc
      661 atcgcattct gactgacgtg acaatgggta atcgcgatag ctaatgcatc ggcagcatcc
      721 gcctgtggat tagcgggcag tttcagcaag gtgcggacca tatgctgcac ctggcttttt
      781 tcagcactac caatacctac caccgtttgc tttacctgac gtgccgcata ttcaaatacc
      841 ggcaattcct gattcaccgc cgccacaatc gccacgccgc gcgcctgacc cagtttcagg
      901 gctgagtcag cgttcttcgc cataaagact tgttcaatgg cgaaataatc aggctcgaac
      961 tgggtgatga tttccgtcac gcccgcatag atgagcttca gacgagacgg taaatcatcc
     1021 actttggtgc gtatgcatcc gctacccagg taggagagtt gcctgcctac ctggcggatg
     1081 acgccatagc cggtcacgcg cgaacccggg tcaatgccga gaataatagc catcacgcgt
     1141 ctccgttttg ctgtttagca ggccccatca gagagtcgct gcaacctcat cggagatttc
     1201 accgttatgg tagacttcct gcacgtcgtc gcaatcttcc agcatatcga tcagacgcat
     1261 cagtttcggt gcggtttcag catccatatc agctttggtg gacgggatca tggaaacttc
     1321 cgcgctgtct gctttcagac ctgccgcttc cagagcgtcg cgtactttac ccatttcttc
     1381 ccatgcagtg tagacgtcaa tcgcgccgtc atcataggtc acaacgtctt cagcacccgc
     1441 ttccagagct gcttccatga tggtgtcttc atcgcctttc tcgaaggaga tcacgccttt
     1501 tttgctgaac agataggcta cggaaccatc agtaccgagg ttaccaccac atttgctgaa
     1561 tgcatgacgc acttcagcaa cggtacggtt gcggttgtca gacagacatt caatcatgat
     1621 tgccgtgccg ccaggaccgt aaccttcgta gatgatggtt tccatgtttg catcatcatc
     1681 accgcccaca ccacgtgcaa ttgcgcggtt cagagtgtca cgggtcatgt tgttagacaa
     1741 tgctttatca attgctgcac gcagacgcgg gttagcgtcc gggtcaccac cgcccagctt
     1801 agccgcggtt accagctcac gaatgatttt agtgaagatt ttaccgcgct tagcatcctg
     1861 cgcagcttta cgatgtctgg tgttggccca tttactatga cctgccataa aaatatctcc
     1921 agatagccct gcctgttcag gcagcgttaa ttacaaactg ttcaatcgcc tgccggttgc
     1981 tccaggactt agtgagcgcc gccgcagcag gcgcatcaag ccacttgtag gccagatgtt
     2041 cagtgaagac gacctggcgt tcgtgcggga gcgcaagaca gaaccatgat tccgtattac
     2101 gcgtcacgcc cggcgcatag cgatgacgta aatgtgaaaa aatttcaaat tctaccgtgc
     2161 gctgacagtc aattaaggtc agttgttcag cgacaacatc gatggtgacc tcttccttta
     2221 cttcgcgcat ggcagcttgc ggcgcggttt caccctcttc cacgctgccg gttaccgact
     2281 gccagaaatc gggatcgtca cgccgctgca acatcagcac ccgtttcgta tcttgcgcat
     2341 agatgaccac taagatcgaa acgggacgct tatacacttt atccttcacg ctgcctcttc
     2401 gttgactgcc ttcgctcatc ccattcacat agttatctat gctcatggga gttcactcag
     2461 ttgccgcctc gatgcaacgc gaatgatttc gtgtatttga gtcatatcag ttattctctg
     2521 ccttcttcac aacctgaatg ctcagctcag ccagtgcagt cgggttagca aagctcggtg
     2581 cttcagtcat cagacacgcc gccgccgtgg ttttcgggaa ggcgataacg tcacggatat
     2641 tgtcggtgcc ggtcagcagc atcgtcagac ggtcaagacc gaatgccaga cctgcgtgcg
     2701 gcggagtacc gtatttcagg gcgtcgagca ggaagccgaa tttctcgcgc tgttcctctt
     2761 cgttgatacc cagaatacca aacaccgtct gctgcatatc accattatgg atacgcacag
     2821 aaccaccgcc cacttcgtaa ccattgatga ccatatcgta agcgttcgcc accgcatttt
     2881 ctggtgcagc tttcagttct gcagcagtca tgtctttcgg tgaggtgaac ggatggtgca
     2941 ttgccgtcag gccgccttca ccgtcgtctt caaacatcgg gaaatcgata acccacagcg
     3001 gtgcccattt gctttcgtcg gtcagaccaa ggtctttacc cactttcagg cgcagtgcgc
     3061 ccatcgcgtc ggcaacaatt ttcttgttgt cggcaccgaa gaaaatcata tcgccatctt
     3121 gcgcggcagt acgatccagg atggcttcga tgatttctgc attaaggaac ttcgctaccg
     3181 ggctgttgat accttccaga cctttcgcgc gttcgttaac tttgatgtaa gccagacctt
     3241 tcgcgccgta gattttaacg aagttaccgt attcgtcgat ctgcttacgg gtcagcgatg
     3301 cgccacccgg aacacgcaga gcggcaacgc gccctttcgg attgttcgcc ggacctgcaa
     3361 atactgcaaa ttcaacagat ttcagcagat cggcaacgtc agtcagttcc atcgggttac
     3421 gcagatccgg tttatcagaa ccataacggc gttctgcttc cgcaaaggtc attaccggga
     3481 aatcgcccag atccacgccc ttcacttcca gccacagatg acgcaccagc gcttccatca
     3541 cttcacgcac ttgtggcgcg gtcatgaaag aagtttccac atcgatctga gtaaattcag
     3601 gctgacgatc agcgcgcagg tcttcgtcac ggaagcattt aacgatctga tagtaacggt
     3661 caaagccgga catcatcagc agctgtttga acaactgtgg ggattgcggc agtgcgtaga
     3721 atttaccttt gtgcacacga gaaggcacca ggtagtcacg cgcgccttcc ggcgtggctt
     3781 tggtcagcat cggagtttcg atgtcgagga agccgtggtc atccataaaa cggcgcacca
     3841 ggctggtgat tttagcgcgg gttttcaggc gctgagccat ttccgggcga cgcaggtcga
     3901 ggtagcggta tttcagacgc gcttcttcgg tgttgacgtg gttagagtca agcggcagaa
     3961 catctgcacg gttgatgata gtcagcgagg acgccagtac ttcgatttcg ccagtcgcca
     4021 tatcgcggtt aatatttttt tcgtcacgcg cacgtacggt gcccgtgacc tgaatgcaga
     4081 actcattacg cagttcagag gccagcttta acgcgtccgc acgatccgga tcgaaaaata
     4141 cctgcacgat accttcgcgg tcgcgcatat cgatgaagat caggctacca agatcacgac
     4201 gacggttgac ccaaccacac agagtcacct gctgccccac gtgggacaaa cggagctgtc
     4261 cacaatattc tgtacgcatg agatatccct taacttagct gccggcggat gccccctgct
     4321 gcgcaggtga ccaagtcgca gcgttagctg tatgtcacaa ctgaatgaaa aaaggcggct
     4381 attatactgg aaattctgcc gcaccgtaag agtctagccc gcgctggaac gcctcgttac
     4441 cactttatat cgggcctgaa atcagactct acgccagttt gctataaagg tgttgcccga
     4501 actcataaaa attaacaaaa tttgtcgttc cgccatcggc taatcgcatt aaggtgagag
     4561 gcacgatttt gttttgtcag gagtcatcat gcttgaactt aatgctaaaa ccaccgcgct
     4621 ggtggtgatt gatttacaag aaggcatctt accttttgcc ggaggtccac atactgccga
     4681 tgaggtggtt aatcgcgccg ggaagctggc ggtgaaattt cgcgccagcg gtcagcccgt
     4741 gtttctggtg cgcgttggct ggtctgccga ttacgccgaa gcattaaaac agccggttga
     4801 tgccccttcc cccgctaaag tgttgcccga aaactggtgg caacatcctg ctgcattagg
     4861 tgcaactgac agtgatatcg aaatcatcaa acgtcagtgg ggggcgtttt acggtacaga
     4921 tctggagttg caattacgcc gccggggtat cgatacaata gtattatgtg ggatctcgac
     4981 caatatcggt gttgaatcca ccgcccgcaa tgcctgggaa ctcggtttta atctggtgat
     5041 tgccgaagac gcctgcagcg ccgccagcgc cgagcagcac aataacagca tcaaccatat
     5101 ctacccacgc atcgcccgtg tgcgcagtgt ggaagagatc ctcaacgcat tatgatttac
     5161 atcggtctgc cgcaatggtc gcatcctaaa tgggtgcggt tggggatcac cagccttgaa
     5221 gagtatgccc gccactttaa ctgtgtggag ggcaacacca cgctttacgc cctgccgaaa
     5281 tccgaggttg tcctgcgctg gcgtgagcag accacagata attttcgctt ctgttttaag
     5341 tttccggcga ccatttcgca tcaggcagca ttacggcatt gcgatgattt agtgactgaa
     5401 tttttgaccc gcatgtcacc gttagcgccg cgcattgggc aatactggct gcaactgcct
     5461 gccacatttg gcccacggga tctgcctgcg ctttggcatt ttctcgattc ccttcccggc
     5521 gaatttaatt atggtgtgga agtccgccat ccacagtttt tcgccaaagg ggaagaggaa
     5581 caaacgctta atcgcggttt acatcagcgc ggcgttaatc gggtgatttt agacagccgc
     5641 ccggttcatg cagcacgtcc acacagtgaa gctattcgcg acgctcaacg aaaaaaacct
     5701 aaagttccgg tacatactgt actgacggcg acaaatccgc tgatccgttt tatcggtagt
     5761 gatgatatga cgcaaaaccg ggaattattt caggtctggt tacaaaaatt agcgcagtgg
     5821 catcagacca ctacgcctta tcttttttta catacgccag atattgccca ggccccggaa
     5881 ctggtacata ccctgtggga agacttacgt aaaacgcttc cagagatcgg agcagttccg
     5941 gctattccac agcaatcttc tcttttctga atttgccacc tatcatagac aggtgccatc
     6001 ggccatttta aagggagttt gtatggtaag cgcgctgtat gccgttttaa gtgcgttgtt
     6061 attaatgaag ttctcttttg atgtcgttcg cctgcgaatg cagtaccgcg ttgcctatgg
     6121 cgacggcggt tttagcgaac tgcaaagcgc tattcgcatt catggtaacg cggtggaata
     6181 tattcccatc gcgattgtgt taatgctgtt tatggaaatg aatggcgcag aaacctggat
     6241 ggtgcatatt tgcggcatcg ttttgcttgc tggtcgtctg atgcattatt acggttttca
     6301 tcaccgtctg ttccgctggc gacgttccgg catgagcgcc acctggtgtg cgctgttgct
     6361 gatggtgctg gcgaatcttt ggtatatgcc ctgggagttg gttttctccc tgcgttagcg
     6421 cacaatacgc cactttcttt ttcccggatt tttacgttat gtctcaccgc gacacgctat
     6481 tttctgcccc tatcgccaga ctgggcgact ggacctttga tgaacgggta gctgaagtct
     6541 tcccggatat gatccagcgt tccgttcccg gctattccaa tattatttcc atgattggta
     6601 tgttagccga gcgcttcgtt caacctggta cgcaggttta cgatctgggt tgttctctgg
     6661 gcgcggcgac gctctcggtg cgtcgcaaca ttcatcatga taattgcaaa attattgcca
     6721 tcgacaactc tccggcgatg attgaacgct gccgtcgtca tattgacgcc tataaagccc
     6781 ctacgccagt agacgttatt gaaggtgata ttcgcgatat cgccattgaa aacgcatcga
     6841 tggtggtgct gaattttacc ctgcaattcc tggaaccttc cgagcgtcag gcgttactgg
     6901 ataaaattta tcaagggctg aacccgggcg gtgcgctggt gctttcggaa aaattcagtt
     6961 tcgaagatgc caaagttggt gaattgctgt ttaacatgca ccacgacttt aaacgtgcca
     7021 acggttacag cgaactggag atcagccaga agcgcagcat gctggaaaac gtgatgctaa
     7081 ccgattctgt ggaaacccat aaagcacgcc tgcatcaagc tggttttgag catagcgagc
     7141 tgtggttcca gtgctttaac tttggttccc tggtggcatt aaaagcagag gacgctgcat
     7201 gatcgacttt ggtaactttt attctctgat tgccaaaaat catctttcac actggctgga
     7261 aacgctgccc gcgcagattg ctaactggca gcgcgagcag cagcacgggc tgtttaagca
     7321 gtggtccaac gcggtggaat ttctgcctga aattaaaccg tatcgtctgg atttattgca
     7381 tagcgtgacg gcagaaagcg aagagccact gagcgccggg caaattaaac gtattgaaac
     7441 gctgatgcgc aacctgatgc cgtggcgcaa agggccgttc tcactgtatg gcgtcaacat
     7501 cgataccgaa tggcgttccg actggaaatg ggatcgcgtt ctgccccatc tttctgattt
     7561 aaccgggcgc accattcttg atgtcggctg tggcagcggt tatcacatgt ggcgcatgat
     7621 tggcgcaggc gcgcacctcg cggtgggaat cgaccctacg caactgtttc tgtgccagtt
     7681 tgaagcggtg cgtaaactac tgggtaacga tcagcgcgca catttgttac cgttaggcat
     7741 tgaacagctt ccggcactga aagcctttga taccgtcttt tcgatgggcg tgctttatca
     7801 tcgtcgttca ccgctggagc atctctggca gttgaaagat caactggtca atgaaggtga
     7861 actggtgctt gaaacgctgg ttattgatgg tgatgagaac acggtgctgg tgccgggcga
     7921 tcgttacgcg caaatgcgta atgtctattt catcccttcc gcgctggcgc tgaaaaactg
     7981 gctgaagaag tgtggttttg ttgatattcg cattgcggat gtgagcgtta ccactacaga
     8041 agagcagcga cgcactgaat ggatggttac cgagtctctg tccgatttcc tcgacccaca
     8101 tgatccgagt aagacagttg aaggttatcc ggcacctaaa cgcgcggtgc tgattgcgcg
     8161 caagccgtaa agattagcgc ccgtttccgg gcgcttttca cgtcttatgc ctgaacagct
     8221 ggctgatcaa acgccgttaa cttcggtgca ttgcccgtat atttttcgat atacaccagc
     8281 gcagagttac cggcacaggc atttgccagc tgagagctgg ggatatccgc cgttaacacg
     8341 ttcgcactgc cgtttttaca caagccattt tccagatctg gccatgcacc ttcatgcacg
     8401 cataccacgc cttttttgat cccgtcagtc accaccgcgc ctgtcagaat ctgaccacgt
     8461 ctgttccaga cacgcaccag atcgccattc gcaataccaa agcgagcagc atcttcggtg
     8521 tgaatagtga tcggttcacg atcggcaacc gcatattttt tacgcagttc cgcatagtta
     8581 agctgactgt gtaaacggtg tgccggatgt gcggtcagaa gctgcaactg cttctcgtcg
     8641 gcggaacctt tccactcatc aggcgcaagc caggttgggt gtgccgggca atccttatag
     8701 ccaaattttt ccagcgtttt ggagtaaatc tcaattttgc cgcttggcgt acccagcgca
     8761 tttttcaccg gatcggcgcg gaaatcacca taacgaacgt actgttcgtt cttctcgctg
     8821 cggcgcattt cgatcagttt attttgctgc cagaaagcat taaacatcgg catagtcacg
     8881 cgttgcgcac gcgcaccttt ctgagcggca tcatagaaaa atttcagcca cgccatttca
     8941 tctctacctt cggtatagat ctcttttccg ccaggtttga gtaattcagc aagatcggca
     9001 aacacgtcaa aatcgttacg cgcttcaaat tgcgcagcga cagcctgctt catcggtaca
     9061 atatgctggt tgctgtaatc accggtcatc gtcaagtcat tgcgctcaaa cgatgtggtg
     9121 atcggtaata cgatatctgc atgtttagca gctgcggtcc agtagcattc agaaacgacg
     9181 atcatctccg gtttctgcca tgctttaatc agacgattag tatcctggtg atgggtaaag
     9241 ttaccgccac ccgcccacca gatcattttg atatttgggt acgtttgctc tttaccgtta
     9301 tgctgatatt tgccgccagg attttccagc gcatcgacga tacgggcaac aggaatcgcc
     9361 gtcattccgc catcatcagc ggcctcgctg gcctgtccgg caatcgccgc agacatttcc
     9421 ggcagcacgc cgccaacacg cgtcgggttg ccaccattgg agtaatgata agagaaacca
     9481 aacccgccgc ccggtgtacc aatctgccct aacatcgccg ccagcgtcac cagcatccag
     9541 tgtttctgtt caccatattg ctggcgctga attccccagc ctgccatcag tattgtgcga
     9601 tttgccgcca tcagttcagc gagtttgact atctgcgctt ccggtacgcc agaaatttcc
     9661 gccgcccagg ccgcgctctt cggcgtatta tcgctcttgc ctgccagata ctcttcaaac
     9721 tgcggatatc cggtagtgta tttttcaagg aacactttat cgtgtttgcc ttgtgtcatc
     9781 agggtatgcg cgatccctaa catcagtgcc acgtcggtgc ccatattcgg cgcgatccag
     9841 gtggcattat cgccaaagaa ttcgatggtt tcggagcgaa taggatcaat ggcaatcacg
     9901 ggtttgccag attttttcag ctgatggaag tattccagcc cttgctcatc ggtactgctc
     9961 caggcaattt tcaaggtatt cagcgggttc atcccccaca gcaccacaac ctggctgttt
    10021 tccagaatca gcggccagga ggtctgctgt tcatacacct ctacagaacc gaccacatgc
    10081 ggcatgatca cctgtgctgc cccggttgaa taatcgccgc tatgcccgga ataaccgccc
    10141 gcaaggttca tataacgttg cagtaaggtt tgcgctttat gcaacacgcc agaagatcgc
    10201 cagccgtaag atccggcaaa aatggccgat ggtccgttag ctttacgaat acgatcgtgt
    10261 tgctcatgaa tcagttttaa tgcttgttcc cagctcacct gtacataggt atcttcgcca
    10321 cgacctttcg ccggttgcag tggattatcg agatagcttt ttctcaccat cggatgctga
    10381 atacgcgccg tggtgtgtac ctgatccacc gccgtagact gtaaggagtt cggtatagtt
    10441 ttcgccagcg cgcctgttga agaaacaatc ttgccgtcct tcacttctac gttcatcgct
    10501 ccccaacggc ccgcggtgag gattttgcca cccttctctt ctgcccatgc gggtagcggt
    10561 gcagccgatg tcaccaccag cgccccagcg gcaataccgc tgtgtttaat aaattcacgt
    10621 cttgttaatg tcataacttc ctccctgatc aacgaggatc actgtttctc ggtaatatct
    10681 ttggcgttgt actggaagta ccgcgtcaaa atgtccagtt cgttttcgct catgctggtt
    10741 cgcgccccca ttcctttggc aatggacggc catgcattga cggtgtaatg gtcggaggca
    10801 ataggggcat ggcaaccagc gcaataggta tcgtcaagtt tttcagcgta ttgccatagc
    10861 ggtttacggt ccgctaatgc gggatcggta agcgcaccct gtaaagacgc ctgacgccat
    10921 tgattgccgt attcgtcagc ctgccattcc ccgtttaccg tgagcgcctt gataccttct
    10981 tcacttaatg tggctagtgc cagccgttga cctgccgcca ggtagagcgt gttttcactg
    11041 ccctgcattt gataaccctg caacagaacg atcggctgtt tgccactggc atcaacgacg
    11101 atgagatcgg taccaggatt cacggtagcc agctcgccta tgcgagaagt tttgaaagga
    11161 taaatatgta cgccattagt cactgaagta gcggcctgac tttccagctc atgcgccgcg
    11221 ttgtcatcca tttttatttc tggcggaaaa tgagcaatgc ctttatgaca atcgatacag
    11281 gtttcgccgc ccttttgtgc tttgttatgc attttctgcg cagattcact ttgcgaggca
    11341 atatccatgg catcaaaaga atggcaacta cggcacgttg cagagtcagt ggcttttaat
    11401 tctttccata ctgtttcggc catttcctga cgatgcgctt cgaacttatc gtcactgtct
    11461 attttgccgc taacaaattc atgataaata tctttagatg cttttaattt agcaaataaa
    11521 taatccatcc ctgactttgg aatatggcaa tcggcacatt ccgcacgtat ccctttctgg
    11581 ttcgaaaagt ggacagttcc ctgatattcc tcaaaaggtt tactcatcga gtggcaggaa
    11641 aggcaaaatg ctgtatccga cgttttatgt aaggcttttt gcgccagcaa taacccgccg
    11701 ccaccaacca caaccgctat cagcaaaaac at
//
LOCUS       DACBVI010000135        11499 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.136, whole genome shotgun sequence.
ACCESSION   DACBVI010000135 DACBVI010000000
VERSION     DACBVI010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.136"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            191..1858
                     /gene="fdrA"
                     /locus_tag="IU325_17920"
     CDS             191..1858
                     /gene="fdrA"
                     /locus_tag="IU325_17920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HAP5810880.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLALGSGSSQAL
                     TQVRRWDSACQKLPDASLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVIPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVSGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHQHGMMLDADGHQILDLGDDFYTVGRPHPMI
                     DPTLRNLLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARPDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPATQQHTPSLL
                     ENVAVINIGLRSFALALQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            1868..3127
                     /locus_tag="IU325_17925"
     CDS             1868..3127
                     /locus_tag="IU325_17925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HAP5810881.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMDRGIDLTAMMAQGITMGDEFHQRNIASSALLMRTLAPQIARLDHDKQSIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQDLANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            3138..3953
                     /locus_tag="IU325_17930"
     CDS             3138..3953
                     /locus_tag="IU325_17930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HAP5810882.1"
                     /translation="MTIIHLLLASRSAPNYCQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDTRSGRQFFACSDNLQLVTTAVSVNYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLMALGHTSGADTLLGFWLGQQLLQGKP"
     gene            3950..4843
                     /locus_tag="IU325_17935"
     CDS             3950..4843
                     /locus_tag="IU325_17935"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP5810883.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTDDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(4980..6047)
                     /gene="purK"
                     /locus_tag="IU325_17940"
     CDS             complement(4980..6047)
                     /gene="purK"
                     /locus_tag="IU325_17940"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP5810884.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFEMHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFN"
     gene            complement(6044..6553)
                     /gene="purE"
                     /locus_tag="IU325_17945"
     CDS             complement(6044..6553)
                     /gene="purE"
                     /locus_tag="IU325_17945"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP5810885.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(6670..7392)
                     /gene="lpxH"
                     /locus_tag="IU325_17950"
     CDS             complement(6670..7392)
                     /gene="lpxH"
                     /locus_tag="IU325_17950"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HAP5810886.1"
                     /translation="MATLFIADLHLCAEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRQVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(7395..7889)
                     /gene="ppiB"
                     /locus_tag="IU325_17955"
     CDS             complement(7395..7889)
                     /gene="ppiB"
                     /locus_tag="IU325_17955"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HAP5810887.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            8063..9448
                     /gene="cysS"
                     /locus_tag="IU325_17960"
     CDS             8063..9448
                     /gene="cysS"
                     /locus_tag="IU325_17960"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP5810888.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(9484..10005)
                     /locus_tag="IU325_17965"
     CDS             complement(9484..10005)
                     /locus_tag="IU325_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810889.1"
                     /translation="MPTVVTHAAVPLCIALGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWVFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(10113..10325)
                     /gene="ybcJ"
                     /locus_tag="IU325_17970"
     CDS             complement(10113..10325)
                     /gene="ybcJ"
                     /locus_tag="IU325_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HAP5810890.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(10327..11193)
                     /gene="folD"
                     /locus_tag="IU325_17975"
     CDS             complement(10327..11193)
                     /gene="folD"
                     /locus_tag="IU325_17975"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HAP5810891.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
BASE COUNT         2630 a         3027 c         3261 g         2581 t
ORIGIN      
        1 taacctggtt tttaaaacaa ccacgcttat taaaaaataa cacgtaatag catggcggtt
       61 atttgaattc ttttgttaat aatccctgtg tgatattcat caccttattt acccgttgtc
      121 atcgataccg taatcgccac attaacactg ctcgtgcaat tgccatgggt gcaattttta
      181 aggagttgtt atgatccacg cctttattaa aaaagggtgt tttcaggatt cggtcagttt
      241 aatgattatt tcacgaaaac taagcgaatc agaaaatgtt gatgatgttt ccgtaatgat
      301 gggtacgccc gccaataaag cgttattaga taccacaggt ttctggcatg acgattttaa
      361 taacgccacg ccgaacgata tttgcgtggc aattcgtagc gaagcggcgg atgcggggat
      421 cgcgcaggcg attatgcagc agcttgaaga ggcgctaaaa caactggcgc tggggtcagg
      481 cagcagccag gcgttgacgc aggtgcgtcg ctgggacagt gcctgtcaga aattacccga
      541 cgccagtctg gcgctgattt cagtggctgg cgagtatgcg gcggagctgg cgaatcaggc
      601 gctggatcgc aacctcaacg tgatgatgtt ctctgataac gtcacgctgg aagatgaaat
      661 ccaacttaaa acccgcgcgc gggaaaaagg cttgctggtg atggggccgg actgcggtac
      721 gtcgatgatt gccggcacgc cgctggcttt tgctaacgtg ataccggaag gcaacattgg
      781 cgtcattggc gcttccggta ccgggattca ggaactgtgt tcgcagattg cgctggcagg
      841 tgagggaatt actcacgcga ttggccttgg cgggcgcgat ctcagccgtg aagtgagcgg
      901 catcagtgcg ctaacggcgc tggaaatgct cagtgcagac gagaaaagcg aagtgctggc
      961 atttgtttca aaaccacctg ccgaagctgt gcgtctgaaa attgttaatg ccatgaaagc
     1021 aaccggcaaa ccgacggtgg cgctgttttt aggttatacc ccggcggtgg cccgcgacga
     1081 gaatgtctgg tttgcctcct cgctggatga ggccgcacgc ctggcttgcc tgctttcacg
     1141 cgtcacggcg cgacgtaacg caatagcacc tgtcagcagc ggatttattt gcggtttgta
     1201 taccggcggt acgctggctg ctgaagcggc gggattactt gccggacacc ttggcgtgga
     1261 agccgacgat acccatcaac atggcatgat gctcgacgcc gacggacatc agatcctcga
     1321 cctgggcgat gatttttaca ccgtcgggcg tccccatccg atgatcgacc cgaccttacg
     1381 caacctgtta attgccgatc tcggcgctaa accgcaagtg cgcgtgttgc tgcttgatgt
     1441 cgtgattggc ttcggtgcga ctgccgatcc tgccgcctcg ctggtgagcg cctggcaaaa
     1501 agcctgtgcc gcgcgtccag acaatcaacc actgtatgcc attgccacgg tgacaggcac
     1561 cgaacgtgac ccgcaatgcc gctcgcaaca aatcgccacg ctggaagatg cggggatcgc
     1621 ggtcgtgagt tcgctgccgg aagccacctt gctggcggca gcgttaattc atccgctctc
     1681 gcctgccaca cagcaacaca caccgtcatt actggaaaac gtcgccgtga ttaacatcgg
     1741 attacgcagc tttgcgctgg cgctacaaag cgccagcaaa ccggttgtgc attaccaatg
     1801 gtcgccagtc gccggcggca ataaaaaact ggctcgttta ttagaacgtt tgcaataagg
     1861 ggttcccatg tttacatcag tggcgcaagc caatgctgcg gttatcgaac aaattcgtcg
     1921 cgctcgtcca cactggctgg atgtgcaacc ggcttcttca cttatcagcg aactaaacga
     1981 gggcaaaaca ctgcttcacg ccgggccgcc aatgcgctgg caggagatga ccggacccat
     2041 gaaaggggcg tgcgtgggcg catgtctgtt cgaaggctgg gcgaaagatg aagcgcaggc
     2101 gctggcaata ctggagcagg gggaagtgaa cttcattcct tgtcaccatg tgaatgccgt
     2161 agggccaatg ggcggtatta cttctgccag tatgccgatg ctggtggtcg agaacgtgac
     2221 cgacggcaac cgggcgtact gcaacctcaa cgaaggtatc ggcaaagtga tgcgttttgg
     2281 cgcttacggc gaagatgtcc tgactcgcca tcgctggatg cgcgatgtgt taatgccagt
     2341 attaagcgcg gcgctggggc gcatggatcg cggtatcgat ctcacggcaa tgatggcgca
     2401 gggcattacc atgggcgatg agttccatca acgcaatatt gcttcctcag cactgttaat
     2461 gcgtacgctg gccccacaaa ttgctcgcct tgatcatgat aaacagagca tcgccgaagt
     2521 gatggatttc ctcagcgtga ccgatcagtt cttccttaac ctcgcgatgg cttactgcaa
     2581 ggcggcgatg gatgctggtg cgatgatccg cgcaggcagc atcgtcacgg caatgacccg
     2641 caacggcaat atgttcggga ttcgggtcag cgggctgggc gaacgctggt ttactgcgcc
     2701 tgtaaacact ccgcaaggtc tgtttttcac cggcttctcg caggatctgg cgaacccgga
     2761 tatgggcgat agtgcgatta ccgaaacctt tggtatcgga ggtgcggcaa tgatcgcagc
     2821 gcctggcgta acgcgctttg tcggtgcggg tggcatggaa gcggccagag cggtatctga
     2881 agagatggcg gaaatttacc ttgaacgcaa tatgcagttg cagatcccag gctgggattt
     2941 tcagggcgcg tgcctggggc tggatattcg tcgcgtggta gaaaccggca ttacgccact
     3001 catcaatacc ggtatcgccc ataaagaggc ggggatcggg cagattggcg caggcactgt
     3061 gcgggcaccg ttggcctgct ttgaacaggc gctggaagca ctggctgaaa gcatggggat
     3121 aggttgagga acgcgcaatg acgatcatcc atcttctgct tgccagccgt agcgcaccga
     3181 attattgcca gtcctggcgg ttagcgggag tgtggcgacg ggcgattaac ctgatgacgg
     3241 aaagcggcga actgttaacg ctgcatcgtc agggtagtgg tttcggcccc ggaggatggg
     3301 tgcttcgccg cgcgcaattc gatgcgttat gtggtggatt atgcggcaat gaacgaccgc
     3361 aggttgtggc tcaagggatt cgcctcgggc gtttcacggt taaacagcca cagcgttatt
     3421 gtttgctgcg tattacgccg cctgcgcatc ctcaaccgct tgcagctgca tggatgcaac
     3481 gcgcggagga aaccgggctt ttcgggccac tggcgttggc ggcaggcgat ccgctgcccg
     3541 ctgaattacg ccagtttcgt cactgttttc aggccgcgct caatggcgtg aagaccgact
     3601 ggcggcactg gctgggtaaa ggtccgggat taacgccaag tcatgatgac acgctgagcg
     3661 gaatgctgct ggcggcctgg tattatggcg ctttagatac gcgctccggt cgccagtttt
     3721 ttgcctgttc cgacaatctt caactcgtta ccacagcggt gagcgttaat tatttacgtt
     3781 atgccgcgca aggatatttc gcctcgccac tcctgcactt tgttcatgct ctgagttgcc
     3841 cgaaacgtac cgctgttgcg attgattcgc tgatggcgct ggggcatacg tcaggggcag
     3901 atacgctgct ggggttctgg cttggtcaac aattattaca aggaaaacca tgaaaacact
     3961 ggttgtggct cttgggggca atgccttgct tcagcgcggt gaggcgctga cggcagaaaa
     4021 tcaatatcgc aatatcgcca gcgctgtacc cgcactggcg cgcctggccc gttcttatcg
     4081 gctggcgatt gttcacggca acgggccaca ggtggggctg ttggcgttac agaatctggc
     4141 gtggaaagag gtagaaccgt atccgctgga tgtgctggtg gcggaaagcc aggggatgat
     4201 tggctatatg ctggcgcaga gcttgagcgc acagccgcag atgccgcctg tcacgacggt
     4261 gctgacgcgc attgaggttt cgcctgatga tccggcgttt ttgcagccag agaagtttat
     4321 cggtccggtt tatcagccag aagaacaaga ggcactggaa gcggcttacg gctggcagat
     4381 gaaacgtgat ggtaaatatt tgcgccgggt ggtggcgtct ccgcaaccgc gtaaaattct
     4441 cgacagcgaa gccatcgagt tgttgctcaa agaggggcat gtggtgattt gcagtggcgg
     4501 cggcggtgtg cctgtgacgg atgacggagc agggagtgaa gcagtgattg ataaagatct
     4561 cgccgctgcg ttgctcgccg agcagattaa cgccgatggg ctggtgatcc tcaccgatgc
     4621 tgatgcggta tatgaaaact ggggaacgcc gcagcaacgt gccattcgcc atgccacacc
     4681 ggatgagtta gcgccatttg ccaaagccga tggttcgatg gggccgaagg taacggcggt
     4741 gagtggctat gtcagaagcc gtggcaaacc tgcgtggatt ggggcgttat cgcgaattga
     4801 agagacactg gcgggcgaag cggggacctg tatttcgctg tagtgatgag agcatccggc
     4861 aatcggcgca ccatgcctga tgcgatgctt gacgcgtctt atcaggccta caatgggtac
     4921 cggagcggta agccggataa ggcgttcacg ccgaatccgg caagaatatg agcaccaggt
     4981 taattgaact tactctgcgc ccacatcacg ccgctggcat actctggcgg cagcagcggg
     5041 attaaggctt ccagcgtcgc ggtcagacgc gatgtgtcgc tgtcggtcaa attcagatgc
     5101 cccactttac gccccggacg gacttctttg tcgtaccagt gcagatgtac cagcggcagt
     5161 ttcagccagt cataattcac atcgctacca atcaggttga tcatcaccga cggactattc
     5221 actaccggtt gcggtaacgg cagatcggta atcgcccgca gatgcatctc aaactggctg
     5281 atgctggcac cgttttgtgt ccagtgaccg ctgttatgca cacgcggagc cagttcgttg
     5341 atcagcagac cttgcggggt gacaaaacac tccatcgcca tcacgcccac atagcccagc
     5401 tcctgcataa tcgccgacag catctcttcg gcctgcgcct gctgctgcgc gttggcctgc
     5461 gggaaagcga cgctggtgcg caaaataccg tcctgatgca ggttatgcgt cagcggataa
     5521 aacacggtgc taccatcaaa gccgcgcgcg ccaaccagcg acacttcgcc agagaagtta
     5581 atgccctgct cgacaataca ttcgccgtaa cactctgccg gtaactgttc ggtttcatcg
     5641 gcacgtaaac gccattgacc gcgaccgtca tagccaccag tgcgacgctt aacaatcgcc
     5701 agctcaccta aacgatcaaa caccgcaggc cactcgctgc gatcggcaag taactgccac
     5761 ggtgcggtcg gcaggtgaag cttgtcgaaa agctgcttct gagtcagacg gtcggcaata
     5821 atcgggaaca catcgcggtt cacaaatgcc ggatggcgcg ccagctcgcg ggttaatgcg
     5881 gtttccggcc agcgttcgat ctcagcggta atcacgcttt gttgaaaagg caccgccgcc
     5941 ggttcagcgt ccagcccgac cggccagaca gcaatgccta acggttcgcc tgcctgacgc
     6001 agcatacggc ctaactgccc gttaccgagg acgcaaacct gtttcatgcc gcacctcgcg
     6061 ggtccgggtt ttccagcact tcatcagttt gtgctttgcg ccagtcattc agacgctggt
     6121 gcaattcttt atcatgagtc gcaagaattt gtgctgccag taacgccgcg tttgccgcgc
     6181 cagctttacc aatcgccagt gtacccaccg gaatgccgcg cggcatttgt acgatggagt
     6241 agaggctatc gacaccgctc agtgcggcgc tctgtactgg cacgcccagc actggcacca
     6301 gcgttttggc ggcaatcatg ccaggcaggt gcgctgcgcc gcctgcgccc gcaataatca
     6361 cctgataacc gttctcttcg gcactttcgg cgaagctgaa cagtttatcg ggggtgcggt
     6421 gagcagaaac cacttcaacg tggtgcggga cattcaggat ttcgaagatt tcggcggcga
     6481 actgcatggt agcccagtcg cttttggacc ccatcacgat ggcgacacgc gccggattat
     6541 tgcgggaaga catgcgtctt aaaactcctg tggtgcacaa ctctcggctt tagagggcac
     6601 agagaatagc acggaaagag agcaaggaaa acggttgcgt ggctgtgaaa tcagcaaagt
     6661 tgcgggtatt taaaacggaa aatgaatcag ctcaacgtca tccgccgtga ctttcaccat
     6721 tgaaccttcc gtatgccagg cacccagtac cacgcggaaa gcaggttgct gattggcgat
     6781 aagttcatgt actgccgggc gatgggtatg cccgtgaatc agccattgca cctgatgttt
     6841 ttccatcgca ctgaccaccg cgttttggtt aacgtccatg atcgccagcg atttactgct
     6901 gttggcttct ttgctgttcg ctcgcattcg cgcggcaatg cgtttgcgca caaacaacgg
     6961 cagggcgagg aatagcgttt gcagccaggg tttgtggact ttggcgcgaa aagcctgata
     7021 accggcgtca tcggtgcaca gcgtgtcgcc atgcataatc aacacctggc gaccatacag
     7081 ttcgagcacc ttttcttccg gcaataacgt catgccgctt tcacgggcaa agcgtttgcc
     7141 gagcagaaaa tcacggttgc catgaatgaa ataacaggga acgccggaat cggacaccgc
     7201 tttgatcgct gccgccatct ggcgatggag tgggttggga tcgtcgtcgc caatccacgc
     7261 ttcaaacaga tcgccaagaa tatacagcgc gtcggccttg cgggcttccc ccgctaaaaa
     7321 acgcagaaaa ccggcggtga tcgccggttc ttccgcgcag agatgaagat ctgcaataaa
     7381 gagtgtcgcc acgattactc gctaacggtc acgctttcaa tgataacgtc ttcttttggc
     7441 acgtcctggt gcataccgct acgaccggtt gccacacctt tgattttgtc taccacgtcc
     7501 atgccttcaa ccacttcagc aaacacgcag taaccccaac cttgcaggct ttcgccagag
     7561 aagttcagga agtcgttatc aaccacgttg atgaagaact gtgcagttgc agagtgcggt
     7621 gcctgagtac gggccattgc cagtgtacca cgggtatttt tcagaccgtt gttggcttcg
     7681 tttttgatcg gttctttggt ggctttttgt ttcatgcccg gttcaaaacc gccgccctga
     7741 atcataaagc cgttgataac acggtggaaa atggtgttgt tgtaaaaacc ttcgcggcag
     7801 tagtccagga agtttttaac tgtttcaggt gctttatcgt caaaagtttt gatgacaata
     7861 tcgccgtgat tggtgtggaa agtaaccatt tttgcatcct gttccgtttg attggtgctt
     7921 caacccggtt cgggtcatat atagggtggt gttatagcat aaccgcacga tcggatcatc
     7981 tcgcaatgta tgctgattcg cgcgggaaat atgggtatta tacgcaactc aattacccac
     8041 acatgtctaa acggaatctt cgatgctaaa aatcttcaat actctgacac gccaaaaaga
     8101 ggaatttaag cctattcacg ccggggaagt cggcatgtac gtgtgtggaa tcaccgttta
     8161 cgatctctgt catatcggtc acgggcgtac ctttgttgcc tttgacgtgg tcgcgcgcta
     8221 tctgcgtttc ctcggctata agctgaagta tgtgcgcaac attactgata tcgacgacaa
     8281 aatcatcaaa cgcgccaatg aaaatggcga aagctttgtg gcgctggtgg atcgcatgat
     8341 cgccgaaatg cacaaagatt ttgatgcttt gaacattctg cgcccggata tggagccgcg
     8401 tgcgacgcac catatcgccg aaattattga actcactgaa caactgatcg ccaaaggtca
     8461 cgcttatgtg gcggacaacg gcgacgtgat gttcgacgtc ccgaccgatc cgacatatgg
     8521 cgtgctgtcg cgtcaggatc tcgaccagct gcaggcaggc gcgcgcgttg acgtggtcga
     8581 cgacaaacgc aacccgatgg atttcgttct gtggaagatg tcgaaagagg gcgaaccgag
     8641 ctggccgtct ccgtggggcg cgggccgtcc tggctggcac attgaatgtt cggcaatgaa
     8701 ctgcaagcag ctgggtaacc actttgatat ccacggcggc ggttcagacc tgatgttccc
     8761 gcaccacgaa aacgaaatcg cgcagtccac ctgtgcccat gatggtcagt atgtgaatta
     8821 ctggatgcac tccgggatgg tgatggttga ccgcgagaag atgtccaaat cgctgggtaa
     8881 cttctttacc gtgcgcgatg tgctgaaata ctacgatgct gaaaccgtgc gttacttcct
     8941 gatgtcgggc cactatcgca gccagctgaa ctacagcgaa gagaacctga agcaggcgcg
     9001 tgcggcgctg gagcgtctct acactgcgct gcgcggcacc gataaaaccg ttgcgcctgc
     9061 gggtggtgaa gcgtttgaag cgcgctttat tgaagcgatg gacgacgatt tcaacacccc
     9121 ggaagcctat tccgtactgt ttgatatggc gcgtgaagtg aaccgtctga aagcagaaga
     9181 tatggcagcg gcgaatgcaa tggcatctca cctgcgtaaa ctttccgctg tattgggcct
     9241 gctggagcaa gaaccggaag cgttcctgca aagcggcgcg caggcagacg acagcgaagt
     9301 ggctgagatt gaagcgttaa ttcaacagcg tctggatgcc cgtaaagcga aagactgggc
     9361 ggcggcagat gcggcgcgtg accgtctgaa tgagatgggg atcgtgctgg aagatggccc
     9421 gcaagggacc acctggcgtc gtaagtaatt gcgctattgc cggatgcgag ttttcgcatc
     9481 cggttatcgc ctgcgccacc acaacattcc catcagtagc atccccggca accacaccca
     9541 cattaattca gaaataatca cctgatgccc gtacggcgtg gtgtaacgag acaatgcaaa
     9601 cggcgcgact ttaatcacct gccagggggc gaaaaagcgt tcatctgacc acggccacag
     9661 ccagccaacc cctttaccac cagtggttac agaatccagc aagctgtgcg atagcaacga
     9721 gacggttaaa aacacccagc agcgaatcag cccagccctg aaccatcggc gtccaatcag
     9781 cacacataac agcgggacaa caaacgcaaa caccagcgaa tgggtaaacc cgcgatgacc
     9841 aaaaacattg ccataagcaa cgccaaattt aaacgacaac acgtcggcat cgggcagcat
     9901 cgccaggatg attccggcaa ataacagacg cggagggatg actttcgaac ccaaccctaa
     9961 agcaatgcaa aggggaacgg cggcgtgcgt aacaacggtt ggcatgatgg tcgcttcggc
    10021 aaaatgtcga tgctatcagc atggatgaac ggggcgtaga gggcaaaagt ctgaaaagag
    10081 aaccggcctg ttgttacagg ccgggaaagg gatcaggcaa caacctgtac gctgtgaccg
    10141 gcaaaactca ctgtctgccc ggcaacgatt ttgcagcgtt tgcgcgtttc aaccgcaccg
    10201 tcgactttca cctggccttc ggcaatcgcg attttcgcct gcgcgccgct ttcgctccag
    10261 ccttccagtt tcagcaagtc gcacagctca acgtgcggat gtttacctaa agaaaatgtc
    10321 gccatgttac tcaccctgtg gatcatgata ttcaacgcac gcctgtagcg tgttttcaat
    10381 cagcgtggca accgtcatcg ggccaacgcc gccgggaaca ggcgtaatgt atgaggcgcg
    10441 tttagccgcg tcttcaaaca cgacgtcgcc cacaactttg ccattttcca gacggttgat
    10501 gccgacatca atcacaattg cgccttcttt gatccagtca ccgggaataa agcctggctt
    10561 accgacggca acgatcaata gatcggcatt ttctacgtgg tgacgcagat ttttagtgaa
    10621 acggtgagtc acggtggtgg tgcaacctgc cagtagcagt tccatgctca tcgggcggcc
    10681 aacgatattc gatgcgccaa tcaccacggc gttgaggccg aaggtatcaa tgttgtaacg
    10741 ctcaagcagc gtgacgatac cgcgcggggt gcagggacgc agacgcggcg cgcgctggca
    10801 cagacgaccg acgttgtaag gatggaaacc gtccacgtct ttgtccggat gaatacgttc
    10861 cagcactttg acgttatcaa tacccgccgg taacggcagt tgaaccagaa tgccatcaat
    10921 ggtgttgtcg gcattcagcg catcgataag ctccagcagc tccgcttcgc tggtggtttc
    10981 cgggaggtca taagagcggg agacgaaccc cacctcttca caagccttgc gtttgcttgc
    11041 gacataaatt tgcgatgcag ggttactacc caccagcaca acggccagtc ctggtgcccg
    11101 cagtccggct gcaatacgcg cctgaacttt ttgagcaact tcagagcgca cctgctgcgc
    11161 aatcgtttta ccgtcaataa tctttgctgc catcagagag aggattccat ctgttacgta
    11221 ggtcgaaggg gatgtgccta ttttgtcaga agcggggcgc gctgtcaggt ttcgtttcag
    11281 atttatcgcg tgaagcgacc tcttgcgaag gtgatgcgca ccgtcgctga gattgaaagc
    11341 ttcatttttc gtccatgatg gcgttgtaaa tctggaactg acttatttcc ttgtcaaagg
    11401 attaagataa tttaagaaat acctgacaat ataaaaagaa ttttcagcct ggtaatttac
    11461 cgcttcaggt ctatatttgt gttgaatata ttttgcgcg
//
LOCUS       DACBVI010000136        11468 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.137, whole genome shotgun sequence.
ACCESSION   DACBVI010000136 DACBVI010000000
VERSION     DACBVI010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.137"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(40..498)
                     /locus_tag="IU325_17980"
     CDS             complement(40..498)
                     /locus_tag="IU325_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HAP5810892.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(554..1405)
                     /gene="manZ"
                     /locus_tag="IU325_17985"
     CDS             complement(554..1405)
                     /gene="manZ"
                     /locus_tag="IU325_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416333.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HAP5810893.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1418..2218)
                     /locus_tag="IU325_17990"
     CDS             complement(1418..2218)
                     /locus_tag="IU325_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose family PTS transporter
                     subunit IIC"
                     /protein_id="HAP5810894.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2281..3252)
                     /gene="manX"
                     /locus_tag="IU325_17995"
     CDS             complement(2281..3252)
                     /gene="manX"
                     /locus_tag="IU325_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP5810895.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3715..5271
                     /gene="yoaE"
                     /locus_tag="IU325_18000"
     CDS             3715..5271
                     /gene="yoaE"
                     /locus_tag="IU325_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HAP5810896.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(5275..6873)
                     /locus_tag="IU325_18005"
     CDS             complement(5275..6873)
                     /locus_tag="IU325_18005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAP5810897.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(7004..8368)
                     /locus_tag="IU325_18010"
     CDS             complement(7004..8368)
                     /locus_tag="IU325_18010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine dehydratase 1"
                     /protein_id="HAP5810898.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8552..9130)
                     /locus_tag="IU325_18015"
     CDS             complement(8552..9130)
                     /locus_tag="IU325_18015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HAP5810899.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(9134..10495)
                     /gene="pabB"
                     /locus_tag="IU325_18020"
     CDS             complement(9134..10495)
                     /gene="pabB"
                     /locus_tag="IU325_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HAP5810900.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAQQDIVLPDMAVGIYDWALVVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10569..10748
                     /locus_tag="IU325_18025"
     CDS             10569..10748
                     /locus_tag="IU325_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HAP5810901.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            complement(10868..11227)
                     /locus_tag="IU325_18030"
     CDS             complement(10868..11227)
                     /locus_tag="IU325_18030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HAP5810902.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMESGERIVIK
                     NPEYFSTYMQEELKALV"
BASE COUNT         2888 a         2879 c         2887 g         2814 t
ORIGIN      
        1 taaaaataca ctaaacatag ctttggctaa attcataact tattgagttg aaacgagaag
       61 cttataaatt ttttccagat cgtcgatatt tcgaacgcgg attaacagcc gacgctgttc
      121 taattgcatc accaacacgc catcttccga caagttcatc gctttgattc ggctatattc
      181 aatccagaca ttggcgaaga aaaaaccttt ttgtttaaag atgatcttcg gaacgcggat
      241 ccagaatata taaaaaccca tcagagccag tgcacttaat aaccatgttg ttattagcgc
      301 gccatgattc gtgacgttgt tatagataag aatgacaatc aatccgacga agataacgct
      361 atcgatgcga ccacgccgga gcaaaggaat tgccagcaga gtggggccgt tacggcgggg
      421 catgatgaac tgatcgtaga tcgcgaaggc caggagtgcg gcgatgaaaa gaatcagtac
      481 caggtccgtg attgtcatta atcctccaga taaaaaaaac ggggccaaaa ggccccggta
      541 gtgtacaaca gtcttacagt cccagcaggc cgcaagcgta accagcgata ccgatgacga
      601 agaagccaac gatgatccac agcgggttaa cttttttgcg cagtagccac atacaagcaa
      661 aggtcagcag cagtggtacc aggcctggca ttaactggtc cagaatagtc tggacagtgg
      721 taacgtgctc tttgcccgtc tggtcagtaa tgcgagagac aaccagcggg atgttgacat
      781 gcgtccactt gttaactaat gcccccatga caaacaggcc gaggatagac gccccttccg
      841 tcagtttttg caggaaacca ccacccatat ctttaacgat atcgatacct ttggagtaac
      901 catacgctac gccgtagtaa cgggttgcca gacgcaccag gttaaacaag atgaagaaca
      961 gcagcggacc taacaggctg ccgctcattg cgataccggc acccagcgct gcaaataccg
     1021 gacgtacggt tccccagaag atcggatcgc ctacaccagc cagcggcccc atcaaaccga
     1081 ctttgatacc gttgatagca ccgtcatcga tctctgcgcc attagcacgc tgttcttcca
     1141 gcgccagggt tacgccgaga atcggcgcag ccacgaacgg ctgggtgtta aagaactcca
     1201 ggtgacggcg aatagcttgt ttacgagctt cgttgttctc agggtagagg cgacgaattg
     1261 ccggtaccat agagaagcag aaacccagtg cctgcatacg ttcgaagttc catgaaccct
     1321 ggaagaggtt agaacgcagg aagacgccac gaatatcact ttgagtgagt tttttctcgg
     1381 tggtagtttg agttgtatca accatttcgc tcacctgtta gtccagttcg ttatcgagat
     1441 cgttgttacc agctgcctga gcaggcgcac cggctacgcg gttgtatttc gggctaagtt
     1501 ggatgtagag cactgccata acagtaccaa tcacacccag agcaaccagg ttaaagttgg
     1561 tgaatgctgc ggttacgaag ccgaggtaga agaacggcat caggtagcca gcacgcatca
     1621 tgttgataac catcgcatag ccaaccacca cgatcatgcc accggcgata ttcagaccat
     1681 tggtcaccac ttccggaatc gcgttcagca tgttctgtac ttcgctggta ccaacagaca
     1741 gcgcaacgat gacggccgga atagccacac gcattgcttg caggaacaga gaagaaacgt
     1801 ggatccagga aatcgctgtc aggttgccgt tatcagcagc cttatccgca gcgtgctgga
     1861 aagcaacggt aatagtacga acgatgatgg tcagtacctg gcccgcagcg gccagaggga
     1921 ttgccagtgc aatacctgca ccaatgctct gatgacctgc gataaccaga atagtagaaa
     1981 tgatggaagc cagagcggcg tcaggcgcaa ctgcagcacc gatgttcatc cagcccagcg
     2041 cgatcatttc cagcgtacca ccgataataa taccggtttt catatcgcca agaacgatac
     2101 ccaccagggt acacgcgatt agcggacggt gaaactgaaa ttcatcgagg attgatccca
     2161 tacctgcgat acaggctacg ataaatacca gcacaatttg aagagtggta atctccattg
     2221 tacttctcct gtttacgtaa gacttaagtg tgaaaactga gtgataatca acacaatacg
     2281 ttacttatcg attttgctga tcagatccat cattttcagt ttcggatcgg tggaaacctt
     2341 acggacttcc agctcaatac cgcgcgcatt cagtttcttg aacgcctcga tatctttttc
     2401 atcaaccgaa accgcgttat tcacctgggt tttaccctga cggaatgcca taccaccgac
     2461 gttaacagag gtgattttca cgccgccttc aacgagacgc tctacatctg ttgggttggt
     2521 aaataacagc attacgcgtt cgccagcata tttcgggttg ttgtagacgc gaatcatttt
     2581 ggcaacatca actacgtgtg ctgttacgcc cggaggtgca acctgggtga gcagtgtctt
     2641 acgaacggta tccgcagcca cttcatcact aacaacaata atacgggaga cattggtttc
     2701 tttggtccag cgggtggcga cctgaccgtg aatcagacgg tcgtcgatac gcgcaaggcc
     2761 aataaccatg tagtcgtttg gtcccattgg ttttgccgga gttggagccg cttttggtgc
     2821 tgcggcagcg ggtgctggcg cggctttttc aaccggtttg gctttcagtg ctttcacgcc
     2881 ttcacggcct gtttctactg ccagcgccac cagttcatca aagcttgggt catcatcacg
     2941 ggccattaac gtttccacga gcattggaat gttaacgcct gcaatgactt cataatgctc
     3001 tttgtcgacg acaatgcggc tggcagcatt gaacgggctg cctccccatg tatcaacgag
     3061 aaacagcacg cctttagtgg tgtcgagttt tgccaactga gcgttgtact tttcaatcag
     3121 cgtttcggca ttttcacctg gaacgaaatc gatccagccg acgttttcct gctcgcctaa
     3181 cagcatttct gccgttttaa gcaactgctc tgcagcccaa ccatgtgtgc ctataacaat
     3241 agcaatggtc acttgctacc tcctttatta tcgttaacac ctcaacgtgc cagatgtatt
     3301 tttgaatcgt cgtccacaat cgaatcgatt cagataaggg ctacaaccaa aaacccctgc
     3361 gtttcgcgat ttattttaga tatcgaaaaa attattttat gtgatgaaga tccgtaattt
     3421 aactttcgat tagcagaaat ttcgaaaggt aaaatatcct tgtcacattc gtttgcaaag
     3481 gaaggtaaat ctttgccaaa tcagaggcgt ctctgatatg tttaactccc gtttaacaac
     3541 catggagtat agggcagtag cccgcagtat ggatcgtcac cgacgtcatt tcagcatcag
     3601 gccttttaac gcctgccttt ctggcactct atgccgcacc tttcgtttgc attttgtcgt
     3661 tacgcctgca ttatttctgg cgtcgaatag ctattcctta agcaggagct tgtcatggaa
     3721 ttcttaatgg acccctcaat ttgggcgggg ctactcacgc ttgttgttct cgaaattgtg
     3781 ctgggtatcg ataacctggt cttcatcgcc attcttgctg acaaactgcc gccaaaacaa
     3841 cgcgataaag cgcgtttgct gggattatca ctggcgctga ttatgcgtct ggggctgctg
     3901 tcgctgattt catggatggt cacgctgacc aaaccgctat ttaccgttat ggatttttcc
     3961 ttctccggac gcgacctgat tatgttgttc ggggggatat tcttgctgtt caaagcaaca
     4021 accgaactgc atgaacggct ggaaaaccgc gatcatgatt ccggccacgg taaaggctac
     4081 gccagtttct gggtggtcgt cacacagatc gtcatccttg acgccgtctt ctcgttggat
     4141 gcggtaatta cggcagtagg gatggttaac catctgccgg tgatgatggc ggcggtagtg
     4201 attgcgatgg cggttatgct gctggcatcg aaaccgctga cgcgatttgt taaccagcat
     4261 ccaacggtgg tggtgctctg tctgagcttc ctgttaatga taggtctgag tctggtggca
     4321 gaaggtttcg gtttccacat tccgaaaggt tacctgtatg ccgcgattgg cttctcgatc
     4381 atcatcgaag tgtttaacca gattgcgcgt cgcaacttta ttcgtcacca gtcgactttg
     4441 ccgctgcgtg cgcgtactgc cgatgccatc ctgcgtttga tgggcgggaa acgtcaggcc
     4501 aatgttcagc acgatgccga taacccgatg ccgatgccga tcccggaagg tgcgtttgcc
     4561 gaagaagaac gttacatgat taacggcgta ttgacgctgg cgtcgcgttc tctgcgcggg
     4621 atcatgacgc cgcgcggtga aataagctgg gttgacgcta atctcggggt cgatgaaatc
     4681 cgcgagcagc tgctctcctc accgcacagt ctgttcccgg tatgtcgcgg tgaactggat
     4741 gaaatcatcg gtatcgtacg tgctaaagaa ctgctggtgg cgctggaaga gggcgttgat
     4801 gtggcggcga tagcttcggc gtctccggca attatcgttc cggaaaccct tgacccgatc
     4861 aacctgctgg gcgtgctgcg tcgtgctcgc gggagctttg ttatcgtgac caacgagttt
     4921 ggtgtggtac aaggtctggt cacgccgctg gatgtgctgg aagccattgc gggtgaattc
     4981 ccggacgcag acgaaacgcc ggaaatcatt accgatggtg acggctggct ggtaaaaggc
     5041 ggtacagatt tgcatgcctt gcagcaggcg cttgatgttg agcaccttgc cgatgacgat
     5101 gatatcgcga cggtcgcggg cctcgtgatc tcggcaaatg gtcacattcc ccgtgtgggc
     5161 gatgtgattg atgtagggcc actgcatatc accatcattg aagccaatga ttatcgtgtt
     5221 gatctggttc gcattgttaa agagcaaccg gcgcacgatg aagatgagta agcgttaacg
     5281 taacggcata atgggcgtga tatgtccatt atgccgggcg ggcggcggtt ggctgcccgc
     5341 cagccatttg ggaaaatcac gtagcggcat cggctgtgcg tacaaatacc cttgcaaaat
     5401 atgcacgcca tgacggcgca aatacttcgc ctgttcttgc gtttccacgc cctccgccac
     5461 cagttcaata ttcagtcttt gccccagcgc gatgatgata tcggtcaccg tcgagttaac
     5521 cgcgtcagaa cctatagctg cggtaaatga cttatcaatt ttcagcacgt caggacgtaa
     5581 tgtttcaagc caggaaaatg aactattgcc tgtgccgaag tcatcaatcg ccagtttgac
     5641 gtttttacga tgcagctcgc gggcaatccg ataatcaaca tccagtaagg catcgcgttc
     5701 ggtgatttcg aggatcagtt gctgaatcgg gtgagcgcta aaccagtact gattgagatc
     5761 ttttatcagc acaccacggc gaaaatgact gggtgcgacg ttaatgccaa catgaaactg
     5821 actactcatt gggaaaacat gccgctgacg gatggtttct gccatcacat agcgggtcag
     5881 tggcacaatt aaatgatgtt cttccgcgat aggaataaac acatccggtg aaatccagcc
     5941 ctgacgtgga ttgttccagc gcagcaaaat ctctacacca atacattgct ggctgcgcgc
     6001 attaagcaaa ggctggcaga acaattcgaa ctcatgttgc gccaggccca gattgatttc
     6061 gcgggaaaaa ctcatccggt aagcggtcgc cagccaggcg atatagccca ccagtaaact
     6121 tagcagcacc gccagcggta attgtgttgg aagatagtgc catgccagcg ccgtagcccc
     6181 cggaccatta acgttaatgg taaaaggaaa gcgttgcgaa gaaacctgat agttttcatt
     6241 gtcttcaggt tgcggaaggg aatctaccag cccattacca tagagcaaat gccgtttgtc
     6301 caccgtcagg ctggcgctac tgatttgcgg cagttgtggc tcaaggagca ttgccgtcag
     6361 taagtcgatg ttaatcatct ccatgacccc agcattgcta ctgcccgctg caggtgtcca
     6421 ttgaatcaaa accggactgc ctttaatcag ggcacggtcg atcgtcaggc gtaaaagtgg
     6481 ttgcggtgca ggaagttcag ccagaatgtc cacgacgggc acattgcgat aaccaaaaat
     6541 gctggagcaa taaagtgtgc cgtcttgcac caggccaatg gatcgcacag tttggagttt
     6601 tgccgcctgt ttacgtaatg gcaaatgggc gacagagcag ggcagaccaa tcagcggaag
     6661 caagacttcg ctacctgcct gtaggggaag cagtacttta tctaattcct ctacagcgtg
     6721 attggcgaat tgtactaccc gttgttgatt taagttacgc tgtgaaataa atcgcacgct
     6781 cagggtcgaa gcgagcgtaa cgagtgcgca aatcgtacaa acaatcattc gattacggcg
     6841 ataggtttta atgatccgtt gtgctttttg catgcgggcc accctgtaag cgtatggcga
     6901 caaacgcctg acgcaacagt ggaagtgtag tggggaatat tacagcagac gagaaaccgg
     6961 ggataaattc gcccatccgt tgcagatggg cgagtaagaa gtattagtca cactggactt
     7021 tgattgccag accaccgcgt gaggtttcgc ggtatttagc gttcatgtcc ttaccggttt
     7081 catacatcgt ttcgatgacc ttatccagcg agacgcgcgg tgcactggtg cgacgcaggg
     7141 ccatccgcgc ggcgttaatc gccttgactg acgcaatggc attacgctca atgcacggca
     7201 cctgaacttg ccctgcaaca gggtcacagg tcaaaccgag gttgtgctcc atgccaattt
     7261 ccgccgccac acaaacctgt tccgggctac cgcccaacag ctcggcaagg cccgcagcag
     7321 ccattgaaca ggcaacaccc acttcgccct ggcaaccgac ttccgcaccg gaaatagacg
     7381 cgttcatttt atacagtgca ccaatcgcgc ccgctgccat aaagtaacgg gtatagatgt
     7441 ccgggctaac cgattcaata aagtggtcat aataagccag cactgccgga acaatgccac
     7501 aagcaccgtt ggttggcgca gttaccacac gaccaccggc ggcgttttct tcgttaactg
     7561 ccagcgcaaa catgtttacc cagtcaatga cattcatcgg atcgttagac agtttatcgc
     7621 tggaaactaa catccggcgc agggccgacg cacgacgcgg cacgcgcagc gggcctggca
     7681 gtacgccttc ggtgttcatc ccgcgatcga tacatgcctg catggtttgc cagacatgag
     7741 cgaaatactc gtcgatctct ttcttgctgt gcagcgccag ttcgttctgc atagcgagac
     7801 cagacagcga atagccggtt tcattgcagt acgccagcag ttcggtggca gatttgaacg
     7861 gatacggcac gctcacttcg ttggcggcat cctgaccaaa gtgttcttca tcgacgataa
     7921 aaccgccgcc gatggaataa taagttttgc tgtagacgac ttcatcgccg ttataggcgt
     7981 ggatttgcat accgttttca tgcagcggca ggttgccgtt atgaaaacgc atcccgttgt
     8041 cgcgcgggaa atccacttca tgccgtccct gtgccagcag cagacgttcg cgctcttcta
     8101 cgtcgcgaat aaaaccggga atactgtcga tatccacggt ggcaggttcg ttacctgcaa
     8161 gacccataat aatggcgata tcggtgtggt ggcctttacc cgtcagcgac agtgaaccat
     8221 aaacgtccac ggcaacgcga gtaacgctat ccagtaagcc tttttcgacc agatcatcga
     8281 cgaactgttt acccgccttc ataggcccta cggtatggga agatgaggga ccaatcccca
     8341 ccttaaacat gtcgaataga ctaatcacga taatactcct gacaaggtga ctggacttcc
     8401 agtaacgaac taataacagc gcatagtgta agagggaacg cggcgactgg cttaactatt
     8461 cacatgaatt aaactaatca aaaaccgcga tttaactaat attgcgatga ttgtctcaag
     8521 tagtgttttg tagatgtaaa gataagtata gtcagggttt cacaccaatt tgcagcgcca
     8581 gctcacgaat tatgcctgcg gtcattcccc atacaaaata ctgttcgtac caggacagcc
     8641 atacccgatg tgaatcgcca cggcggtaga tatctaaagg gtgataacga cccagatgta
     8701 atgcctgggc gagcggcatt tcaaacaccg ccgagacttc atcttcactg gcgcgatacg
     8761 gcagatcggg cgggataatg ccgaccactg gggttacctg gtagccagtt acgctatcga
     8821 cgggcggcag cacgccgata acttcaacgg cggaaggcgg tatagcgacc tcttcttcag
     8881 cttcgcgcag cgcggcggcg ataactgatg catccgtgtc atcgactgcg cctccaggga
     8941 atgccacttg tccagcgtgt ttacgcagat gaattgaacg ctgagtcagc aacaaccccg
     9001 gttgcggtcg acggacgatg gggattaaca cggcagcctg acgatgattt agggtttccc
     9061 ggttaatttg cgggcgcaaa agttgaaagc gcgataaaaa atcatcaagc gtcaggctac
     9121 ggtattccac gtcttacttc tccagttggc gtaatatctt attaacttta tcaaaagttt
     9181 cctgatattc cgcttcttcc tggctatcgg cgacaattcc accgcccgca gagcagtata
     9241 tttgtccgtt aatggcagtc agagtgcgga tagtaatgct ggtgtccatg ttgccgcaaa
     9301 agctcaaata gccaatgctg ccgcaccagg cattacgtcg ctgcggttcc agttcgtcga
     9361 taatttccat agcccgtact ttcggagccc cggttattga gccaccagga aaagctgcgc
     9421 gcagcagatc gctggcgtgt aactgttctg gtagttgcgc cgttatggtg ctgaccagat
     9481 gatgcacggc agggaagggt tccaccacga acagctctgg tacttttacc gaacctgcta
     9541 cggcaacacg accgatatca ttacgcatta aatcgacaat catcagattt tcggcacgat
     9601 ctttcgctga gttcgccagt ttttctgctt gtttgctatc ttcctgagga tcgggcaggc
     9661 gtggtagcgt gcctttaatc gggcgggtct ggatttcact attatcacaa aggataaacc
     9721 gctctggcga aaggcttaaa atcgcaccct gttcaagacg taaaaaagcg ctaaacggcg
     9781 cgcggttggc ctgattaagc tgaaggaatg cctgccattc atcgccagaa taggtcgcat
     9841 gaaaacgctg ggcgagattc acctgatagc aatcaccgct gtgcagatat tcctgtacct
     9901 ggcgaaattt ttcgccgtac tgctcgcggg tcatattgga ttgccagtcg gaagtgagcg
     9961 tgaaatcttc ctgcggggag aattgctggc tttccagcca tgcccgacgg gcattgacat
    10021 cattatgact cagcaaagaa actgtatgac gctggtggtc aacaaccagc gcccagtcgt
    10081 agatacccac tgccatatcc ggcagaacga tatcttgctg cgcaatttct ggcagtgact
    10141 caaaacggcg gcccagatcg tagccaaaca accccagtgc gccgccctga aatggcaaat
    10201 cttcgttatg cgttgggcga atgtctgcgc gatccagcac ctgctggagc acctgtagcg
    10261 ggtcatcagt ggtcgttgtg cgtttttcgc tttcactaac aacggtttct ttaccgaaag
    10321 tggttaaagt gcaaatcggc tcggcgacca caatatcaaa gcggctatat ggatgatcgg
    10381 catagccgga gtgtaaaagc atcgcccacg gcaggtggct taagcgggag aaataaaatt
    10441 cagcggcgtc ctgacgccag ggtaaagtaa tcacagcggg agataacgtc ttcatcagtc
    10501 ctgactctac tggctatgtg ccgggtagca tgaaataatt agcgccaaca atttagcagg
    10561 agttaacaat gtttgcaggt ttaccttcac tcacccatga acagcagcaa aaagccgtcg
    10621 agcggatcca ggaactgatg gcacaaggga tgagcagcgg ccaggcaatc gcgctggtgg
    10681 cagaagagct gcgtgccaac cattccggcg agcgaatcgt ggcgcgtttt gaggatgaag
    10741 acgagtaaca cactaatccg tatcgctttc gttatagaag ccgatgatta tttgattgtg
    10801 agaaaaagga cgcttttcat atgtgattaa aagcgtcctg acccatttat gctgatgtaa
    10861 attttactca gaccagtgct tttagttctt cctgcatata tgttgaaaag tattcaggat
    10921 ttttaatcac aatgcgttca ccagactcca ttttttttgc ccatccaacc attttttccg
    10981 tgaaccccgg gttccagcct tcctggtcag ctcgcgccgt aatatcggcg gcgctcgcgg
    11041 gtactcccag ttcttttaaa tatttaaaga ttcccttcgc cgtgctttca ttcatcgaac
    11101 ttggtgttgg tgctgataca tccatggcac caatgaaatc cagggcttta tctattacag
    11161 caggcatatt cttatcctta aaattaacca tgttatcgat aaggctatat atgaccttaa
    11221 tttgcatcta atatcaaagg gattataacg atatggcacg agttaacctg ttgatattca
    11281 ggtgttttaa aggtggcagt tcatggtgtg ttatccagta agagggagaa aattgacggt
    11341 cagttctcac gctataacca aattgttcag ctaagaaaga aaaccagaga tgctaatcga
    11401 agggcgaaca caacggcatg gttatgttta tttatatagt tagctttata gctaaaattg
    11461 aaattata
//
LOCUS       DACBVI010000137        11451 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.138, whole genome shotgun sequence.
ACCESSION   DACBVI010000137 DACBVI010000000
VERSION     DACBVI010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.138"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(147..953)
                     /gene="trpA"
                     /locus_tag="IU325_18035"
     CDS             complement(147..953)
                     /gene="trpA"
                     /locus_tag="IU325_18035"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HAP5810903.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVTKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKAATRS"
     gene            complement(953..2146)
                     /gene="trpB"
                     /locus_tag="IU325_18040"
     CDS             complement(953..2146)
                     /gene="trpB"
                     /locus_tag="IU325_18040"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HAP5810904.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETDVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2158..3516)
                     /gene="trpCF"
                     /locus_tag="IU325_18045"
     CDS             complement(2158..3516)
                     /gene="trpCF"
                     /locus_tag="IU325_18045"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HAP5810905.1"
                     /translation="MQTVLAKIVADKAIWVETRKQQQPLASFQNEIQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLPI
                     VSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRHLAAVAHSLEMGV
                     LTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFANGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTRGQDARAAYDAGA
                     IYGGLIFVTTSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            complement(3520..5115)
                     /gene="trpD"
                     /locus_tag="IU325_18050"
     CDS             complement(3520..5115)
                     /gene="trpD"
                     /locus_tag="IU325_18050"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HAP5810906.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5115..6677)
                     /gene="trpE"
                     /locus_tag="IU325_18055"
     CDS             complement(5115..6677)
                     /gene="trpE"
                     /locus_tag="IU325_18055"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HAP5810907.1"
                     /translation="MQTQKPTLELLTCEGTYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLAAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6699..6792)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6769..6813)
                     /gene="trpL"
                     /locus_tag="IU325_18060"
     CDS             complement(6769..6813)
                     /gene="trpL"
                     /locus_tag="IU325_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HAP5810908.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6951..7832
                     /locus_tag="IU325_18065"
     CDS             6951..7832
                     /locus_tag="IU325_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HAP5810909.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAISPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7829..8449
                     /locus_tag="IU325_18070"
     CDS             7829..8449
                     /locus_tag="IU325_18070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP5810910.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8477..10372
                     /locus_tag="IU325_18075"
     CDS             8477..10372
                     /locus_tag="IU325_18075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HAP5810911.1"
                     /translation="MAGKFRCILLLIAGLFVSSLSYAENTEIPSYEEGISLFDVEATL
                     QLDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGVPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNKWPF
                     EIYKTRFSLQISNIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLSNAPALQTTNIFSHLLLPSTSSLLIWFPCLLMVCGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQYDKNQQILIRV
                     NEGATRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRRAFLRMQKFYLPRKKSSF
                     YRPDAFLQWGGMAILAVILYGNLSPVGWAGMSLVGDMFIMICWLLPFLFCSLELLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVVLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAVSAN
                     LGREWAERITPSLTAAVSAPEIARSGVLFSLQTHLSCGAYTSLLGRSYSGGGAGGGAG
                     GGGGGGW"
     gene            10583..>11451
                     /gene="rluB"
                     /locus_tag="IU325_18080"
     CDS             10583..>11451
                     /gene="rluB"
                     /locus_tag="IU325_18080"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HAP5810912.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNN"
BASE COUNT         2686 a         2921 c         3079 g         2765 t
ORIGIN      
        1 ttactgttgg gctgaaaaaa gacgtaagtt tacgttcgac aggggtaagc gaaacggtaa
       61 aaagataaat aataaatgaa tttaggattt ttccagcttc gttaaagaca gttaaaatgc
      121 cgccagcgga actggcggct gtgggattag ctgcgcgtcg ccgctttcat cggttgtaca
      181 aaagctttca gtgccgccag cattttctct ggctcattaa tatgttgctc gatgatttta
      241 acgatggccg aaccagaaat cgcgcccgca gctcctgcat caatcgctgc ttttacctga
      301 tccggggcgg aaataccaaa gccctgcaat ggaggcgcag cattgtattc tttcagcttc
      361 gtgaccagat gattgagggg taacgcggcg cggttttctg cgccggtcac gcccgcacgt
      421 gacaataaat aggtgtatcc acgaccgtag gaggctatct ggcgcagcaa atcatcgtcg
      481 gcattcggcg ggcaaataaa gataggtgcg acattatgac gcaacgcggc ctggcggaag
      541 ggcgcggact cttccactgg cacatcggca accagcaccg aatcgacgcc gactttctcg
      601 cactgggcat aaaactcatc aatgcctttg ttaaacacca ggttggcata catcaaaagg
      661 ccgatgggaa tggtcgggtg cttctggcga atcagtgcca gcatctcaaa gcactgtgcc
      721 ggggtcacac ctgccgcaaa ggcacgcagt gtggcgtttt gaatcgtcgg gccatccgcc
      781 agtgggtcgg agaaggggat acctaactcc agcgcgtcag caccggcttc aattagcgtg
      841 tcgataattt tcagcgactg ctcaatgccc ggatcaccga gcgtgacgaa aggaacgaat
      901 gcgccttctt tgcgctcctt caactgggca aacagagatt cgtagcgttc catcagattt
      961 cccctcgagc tttcaaaata tcgtgaacgg tgaagatgtc tttatcgccg cgaccggaaa
     1021 ggttaaccac cagtagctgc tctttttccg ggttttcgcg catcattttc agcgcatggg
     1081 ccagggcgtg ggaggactcc agcgccggga tgatcccttc gtgcaggcag agcgttttga
     1141 aggcttccag tgcttcatca tcggtaattg acacgtaatc agcgcgtcca gtgctgttaa
     1201 gatacgcgtg ttgtgggccg acagacggga aatccagtcc ggcggagatg gagtaagatt
     1261 cttcaatctg cccgtcttcg gtttgcatca tcggcgcttt cataccgaaa tagatgccca
     1321 cacgaccatg ttttaacggt gcgccgtgct cgccagtttc gataccgtga ccgccaggct
     1381 ccacaccaat caggccgacg tcggtttcgt tgatgaaatc agcaaacatg ccgatggcat
     1441 tcgaaccgcc gccaacacag gcgataaccg catccggcag gcgaccttct ctttccagaa
     1501 tctgcgcttt cgtttcttcg ccaatcatcc gctgaaactc acgcacaatg gtcgggtaag
     1561 gatgcgggcc agccgcggtg cccagcatat agtgcgcggt ttcataactg ccggaccagt
     1621 cgcgtagcgc ctcattacag gcatctttca gcgtcgcgga accgctatgc accgggatca
     1681 cttccgcacc cattaagcgc atacgaaaaa cgttaggcga ctggcgctcg acgtctttgg
     1741 cacccatata aatgcgacat ttcaggccga gcagggcgct ggcaagggcc gacgccacgc
     1801 catgctgacc ggctccggtt tcggcgatga tttccgtttt acccatccgc ttcgccagca
     1861 aagcctggcc gagcacctgg ttggttttat gcgcgccgcc gtgcagcaaa tcttcgcgct
     1921 tgagatacag cgtggtgtta gtgccagccg taatgttctg gcatttggtg agtgcggttg
     1981 gacgcccggc atagtttttc agcaggtcgt tgaactgagc ctgaaattca ggatcttttt
     2041 gcgcgctgac aaaagcttct tccagctggc gcagagcagg catcaggatt tgtggcacgt
     2101 acatgccgcc aaactcacca aaatacgggt taagtaatgt tgtcattgtt cctttcctta
     2161 atatgcgcgc agcgtctgga aaaccgaggc caaaagacgt gcgtctttga tgcccggttg
     2221 cgactctaca gcagaattaa aatcaagtcc ggcgcagccg gtttgtgccg cttccacgca
     2281 gttatctgcg cctaagcccc ccgccagcag aacgttgcca agcgattgac cgtttaacag
     2341 tgaccagtcg aaacgttgac cgcttccacc ctggccgttg tctaaaacat atttatcaac
     2401 gtgctgaagc tcgcgggcgg gcagggtttc accgacgctt aatgctttcc agatggcaac
     2461 gtgtgctggc agagcttcac gcagcgtatc gatatacagc tgatcttcat taccatgcag
     2521 ttgcactgcc gccagcgata acaccttagc tttgtccacc acatcggcaa tatcgtgatt
     2581 gcggaacacg ccaacatact gcaacggtgc cgcagccatc acttcctgcg cctgttcaac
     2641 gttgacgcaa cgcggtgacg ttgtaacaaa aatcaaccca ccgtaaatcg cgcccgcgtc
     2701 ataagcggct ctagcatctt gcccacgcgt caggccacat actttattct cacccagcaa
     2761 cacccggcgc acggcggcgt tcaaatcgtc atgggccatc aacgccgaac caatcagaaa
     2821 accgttagcg aagtggctta actcgcgcac ctgagcgtaa gtattgatgc cggattcgct
     2881 gattaccgtc acgttgtgcc ccagtttcgg cgcaagctcg cgggtacggt tgagatcaat
     2941 cgacaaatca cgtagatcgc ggttgttgat gccaacgacc tttgccccca atgcaatggc
     3001 gcgctccagt tcctcttcat tactgacttc ggtaagcacg cccatctcca gactgtgggc
     3061 gacggcggca aggtggcgat attgttcgtc atccagtact gaaagcatta ataagcaggc
     3121 atcggcctgg taatagcgcg ccagatagat ctggtaaggg tcgataatga agtctttaca
     3181 taaaatcggc tgcggggcga tttggctgac gattgggagg aaatcaaagc tcccctgaaa
     3241 atatttctca tcagtcagca ccgaaattgc cgaagcgtaa tgtttataaa tggcggcaat
     3301 gcgtgccgga tcgaaatcat cacggatcac gccttttgac ggcgaggctt tcttgcactc
     3361 aagaataaac gccgtgcgtg caccctgtag cgcatcataa aaatgtcgcg tgctcggctg
     3421 aatctcattc tgaaaactgg ccagcggttg ctgctgtttg cgggtttcta cccaaatcgc
     3481 cttgtctgcg acgattttcg ctaaaacggt ttgcatcatt taccctcgtg ccgccagtgc
     3541 ggtgactctg tcgtaagcgg aaccactgcg cagtacctca agaacagttt gcgcattggc
     3601 ttgcagatct tcatggccat gcaggcgcat taacatggcg acattcgccg cgacggctgc
     3661 ttcatgggcg gcgtcgcctt taccttgtaa caagcgtgtt aaaatgtcac ggttttcttc
     3721 cggtgttcca cctgccagtt gctcctggtg gtagggggtc aggccaaaat cttcagcggt
     3781 caattgatag ctcttaattt cgccgtcatg cagttcggca acgattgtcg gcgcgtgtaa
     3841 tgaaacttca tccatcccgc cgctgtgcac cactgccgcg cgttgatacc ccagcacgcg
     3901 caaggtttcg gcaatcggta gcaccagttc cggactataa acaccaatta acgccagcgg
     3961 aggatgcgcc gggttaatca gtggccccag cacgttgaac agggtgcggg ttttcagttg
     4021 ctggcgaacc ggcatcgcgt ggcgaaatcc ggtgtgatat ttcggcgcaa agaggaaaca
     4081 gacgcctaac tcatccagcg cctggcgcga tttatcggcg ttcatatcaa gattaatacc
     4141 gaatgccgcc agcagatccg acgaaccgga tttactggag acactacggt tgccgtgttt
     4201 cgccactttc agcccacagg ccgcggcgac aaacgcactg gcggtagaaa tattgatact
     4261 gttgctgccg tcaccaccgg tgccgacgat atcggcaaac agataatccg ggcgcgggaa
     4321 tggcgcagca ttttccagaa gggcagttgc tgccccggcg atttcgttcg ggtgctcgcc
     4381 gcgaattttc atgctcacca gcgccgccgc gagctgttct ggcttcagct cgccacgcac
     4441 caccgctgaa aacagctggt ggctttcttg ctggctaagc gtctgcgcct gatacagttt
     4501 ttccagaatc ggttgcagcg tgttagtcgg ttccagtttc tgctgcgccc aggccaatgt
     4561 ttgttccagc aggcgagcgc cctgggtagt aagaatggat tccggatgga actggaatcc
     4621 acaaacgcga tctgcatcgt gacgcaccgc catcaccatg ccattaaaat gggcgttgat
     4681 ggttaaaccg gccggaatgt tactgccaac cagcgagtga taacgcgcca ctggcagcgg
     4741 gtttgttaat ccggcaaaca tcgcctgacc gtcatgttca atgctggagg ctttaccgtg
     4801 aagaatttcg cccgcttgac cgacataccc cccataagct tcgacaatcg cctgatgacc
     4861 gaggcaaatg ccaataattg gcagcttgcc gcgcaggcgg gtgaggagtt ccggcataca
     4921 accggcttca cttggcacac cggggccagg agaaagcatc agcaccggat tgctcattgt
     4981 cgccaggcgt tcaattaagg tctgcgccgg aatatggttg cggtaaatca ccacgttatg
     5041 accattgctg cgcaactgat ctgccaggtt gtaagtaaaa gagtcgatat tatcgagcag
     5101 cagaatgtca gccatcagaa cgtctcctgt gcatgatgcg cggtggcaat agcgcgcagt
     5161 acagcgcggg ctttattacg ggtttcgtcg gcttccgact gcggaacaga atcaagtacc
     5221 acgccagcac cggcttgcac ggtggcgata ccgttttcca ccagcgccga gcggatcaca
     5281 atgcaggtgt cgagatcgcc atgcgcggtg aaataaccta ccgcgccgcc gtagctgccg
     5341 cggcgacgac cttccgcctc ggcaattaac tgcatagcgc gtactttcgg cgcaccgctt
     5401 aacgtcccca tattcataca ggcgcggtaa gcgtgcaggg cgtcgagatc gtggcgcagc
     5461 tcaccgacca cgcgggagac caggtgcatc acgtaagagt aacggtcaac tttggtgaga
     5521 tcggcgacgt agcggctgcc gggggtgcaa atgcgtgcca gatcattacg ggcgagatcc
     5581 accagcatca gatgttcaga aagctcttta tgatcggtac gcatttccag ttcgatgcgg
     5641 ctgtcgaggt ctctgtccag cgagccatcg gcgcgacgac cgcgcgggcg tgttccggca
     5701 atcgggtaga tctcaatctg gcggctggtg gcgtcatact tgagcgaact ttccggcgac
     5761 gcgccaaaca gggtgaaatc attatcctgc ataaaaaaca tgtacgggct gggattactc
     5821 tttttcagca cgtaataggc cgccagcggt gacgggcagg gcagagagaa acggcgagat
     5881 ggcaccacct ggaaaatttc tccagcgcga atcgcttttt gcaacaaacg cactacgcca
     5941 ccgaactctt catcgctctg attacattca caacgcatat gcggcacgga aaccaccggc
     6001 agcggtggcg cggcttcggt cagctgctgg cgaagatcgt tcaggcgagc agtgagacgt
     6061 tgtttttctt cttcattcgg agcaaacagg ctggcctgaa tgcgggtgct ttttttctga
     6121 tggtcaatca ccatcagcgt ttctgcgaga taaaagcaga aatcaggaca gttattttcc
     6181 gctgccagtt gcggtaaatc ttcaaatccc gccacaaggt cataagagaa caggccgccg
     6241 aagaacatgg cttctcgttc ttccttcggt acattcaaca gattctgcaa taaacggaaa
     6301 gcgtcaaaaa cagaaaggga gcataagcgg gcgtcttcat ccagcagtgg actgacaggg
     6361 gggaagcgca gcacacggca gtttggtgat tgttcacttt ccacacccgc aggcagggcg
     6421 ttatccagta gtgccagcag ggcttcgcca ttgccggaaa gtgcctgaat tgtgacagtg
     6481 tcacctaatg ctgtaatgcg cagcgcactg tctatcagca gcaggctttt taaatcatct
     6541 ttgctgtcga tatctgcgga ttccagcagc agcgttgctg gacgattccc acacaactgg
     6601 tgaaaaagcg cagtcgggtt gtcgcgataa gtgccttcgc aggttagcag ttcgagagtc
     6661 ggtttttgtg tttgcattgt tattctctaa ttttgttcaa aaaaaagccc gctcattagg
     6721 cgggctgggt atctgattgc tttacgcatg gtgaatacac tgcccgtttc aggaagtgcg
     6781 ccaccaacct ttcagtacga aaattgcttt cattgtcgat acccttttta cgtgaacttg
     6841 cgtactagtt aactagttcg atgattaatt gtcaacagct catttcagaa tatttgccag
     6901 aaccgttatg atgtcggcgc aaaaaacatt atccagaacg ggagtgcgcc ttgagcgaca
     6961 cgaattacgc agtgatttac gacctgcaca gccataccac agcttccgat ggctgcctga
     7021 cgccagaagc attggtgcac cgtgcagtcg agatgcgcgt cggcaccctg gcgatcaccg
     7081 accatgacac cacagctgca atctcaccag caagagagga aatttcacgt tcgggactgg
     7141 cgctgaatct tattcccggc gtggaaattt ccacggtctg ggaaaatcat gaaattcata
     7201 ttgtcgggct gaatattgat attactcatc cgctaatgtg tgagttcctc gcgcagcaga
     7261 cagaacggcg caatcagcgg gcgcagctga ttgctgaaag acttgaaaaa gcgcaaatac
     7321 ctggggcgct ggaaggcgcg caacgactgg cgcagggcgg tgcggtgacg cgcggtcatt
     7381 ttgcgcgttt ccttgtggag tgcggcaaag ccagttcaat ggcggatgtc tttaaaaaat
     7441 atctggcgcg cgggaaaacc ggatacgttc cgccacagtg gtgtacaata gaacaagcta
     7501 ttgatgtcat tcatcattct ggcggtaagg cggtgctagc tcatcctggg cggtacaatc
     7561 ttagtgctaa atggctgaaa agattggttg cgcattttgc cgaacaccac ggcgacgcga
     7621 tggaagtcgc gcagtgccag caatcgccca atgaacgcac ccagctggcc acacttgcgc
     7681 gtcagcatca tttatgggca tcacaaggat ctgattttca tcagccgtgc ccgtggattg
     7741 aactgggtcg taaactctgg ctgcctgcag gcgttgaagg cgtctggcag ctatgggaac
     7801 agccgcagaa caccacagag agggaattat gagccagttt ttttatattc atcctgataa
     7861 cccgcagcaa cgtctgatca accaggcggt ggagatcgtg cgtaaaggcg gggtgattgt
     7921 ttatccaact gattccggct atgcgctcgg ctgtaaaatt gaagataaaa acgccatgga
     7981 gcgtatttgt cgtattcgcc agctgccgga cggtcacaac tttaccctga tgtgtcgcga
     8041 tctttctgag ctctcgacct attcattcgt ggataacgtt gcgtttcgtt taatgaaaaa
     8101 caacacgccg ggcaactaca ccttcatcct gaaagggacg aaggaggtgc cacgccgtct
     8161 gttgcaggaa aaacgcaaaa ccatcggtat gcgtgtgcct tctaacccga ttgcgcaggc
     8221 gctgcttgaa gcattgggcg aaccgatgct ttccacatca ctgatgctgc caggtagcga
     8281 atttaccgaa tccgatccgg aagaaattaa agaccgtctg gaaaaacagg tagatttgat
     8341 tattcacggc ggctatctcg ggcagaaacc aacaacggtt attgatctta ctgatgatac
     8401 gccggtcgtg gtgcgtgaag gcgtaggtga tgtgaagcct ttcttataac aaattgcgtt
     8461 ccacggatgg aagactatgg cagggaagtt tcgctgcatt ttgctgttga tagcagggct
     8521 gtttgtatca tctctaagtt atgcagaaaa cacggagatc ccttcttatg aagaagggat
     8581 ctcgctcttt gatgttgaag ccaccctgca actggatggg gtgctcgaca tcaaagagaa
     8641 cattcatttt caggcgcgaa atcagcagat taagcacgga ttttatcgtg atttaccacg
     8701 actatggatg cagcctgatg gtgacgctgc actgctgaac tatcatattg ttggcgtcac
     8761 ccgtgatggt gttcctgaac cctggcatct tgactggcat atcggtttaa tgagtattgt
     8821 cgtgggcgat aaacaacgtt tcttgcctca aggtgactat cattatcaaa ttcattatca
     8881 ggttaaaaat gctttcctgc gtgagggaga ttctgatctg ttaatctgga acgtgaccgg
     8941 taacaagtgg ccgtttgaaa tttataagac ccgattttct ctccagatct ctaatattgc
     9001 gggtaatcca tttagcgaaa ttgatctctt taccggagaa gagggcgaca catatcgaaa
     9061 tggccgcatc cttgaggacg gaagaattga atcccgcgat ccgttttatc gtgaagactt
     9121 cacggtcctc tatcgctggc ctcacgcttt acttagcaat gccccggctc tacaaacgac
     9181 gaatattttc agccatcttc ttttaccctc cacgtcatcg ttgttaattt ggtttccgtg
     9241 tctgctcatg gtttgtggat ggttatatct ctggaagcgc aggccgcaat ttacgccggt
     9301 agatgtgatt gaaaccgatg tcattccgcc agattacacg cccggcatgt tacgtctcga
     9361 tgcgaagctg gtttacgacg ataaaggttt ttgtgccgat atcgtaaatc tgattgtgaa
     9421 aggcaaaatt catctggaag atcagtatga caagaaccag caaatcctga ttcgtgttaa
     9481 tgaaggcgcg accagaaata atgcggtatt actgcccgca gagcagttat tactggaagc
     9541 gttatttcgt aaaggcgata aggtcgttct tacggggaga cgcaacagag tgttacgcag
     9601 ggcattttta cggatgcaga aattttatct gccgcgtaaa aagtcttcgt tttaccgacc
     9661 tgatgcgttt ttgcaatggg gcggaatggc aatattggcg gtcattctct acggtaacct
     9721 gagtcccgta ggctgggcag gaatgagtct ggtgggcgat atgtttatta tgatctgctg
     9781 gcttcttcct tttttatttt gttcccttga gcttttgttt gcccgcgatg atgacaagcc
     9841 ttgcgttaat cgtgtaatca tcactttgtt tttaccactg atttgttcag gtgtggcctt
     9901 ctattctctc tatatcaatg tcggagatgt attcttttac tggtatatgc cagcgggtta
     9961 ttttagcgct gttgtcctga ccggttatct cactggcatg gggtatattt ttctgccaaa
    10021 gtttacccaa actgggcagc aacgttatgc ccacggtgaa gctatcgtta actatcttgc
    10081 gcgtaaagag gcggcaacac acagtgggcg gcggcggaaa ggggaaacac ggaaactgga
    10141 ttacgcgttg ttaggttggg cggtctcggc aaaccttggt agagaatggg cagaacgtat
    10201 caccccatca ctcacagcgg ctgttagcgc cccggaaatt gcccgtagtg gcgttttatt
    10261 ctcattgcag acgcacctga gttgcggggc ctataccagt ttgttggggc gaagctattc
    10321 cggtggtggt gctggcggcg gtgcgggtgg cggaggcggt ggtggttggt aaccaaatcg
    10381 acgttaatcg aaacaagctt taaccataaa gatagtgtag tatgttgcgc ctcaaagcca
    10441 ggccgataaa cgtcgagtcg tttacttaag gcctgaagag ttcaaacaga ctttactatc
    10501 agtgagtcag atgtcagttc tgactcactg ttgcacaaaa gcatatcaga cgcctgggaa
    10561 ggcgacacca aaaggaagct ctatgagcga aaagctacag aaagtgctgg cgcgtgccgg
    10621 ccacggttct cgccgtgaaa tcgaatctat tattgaagcc ggtcgtgtga gtgttgatgg
    10681 caaaattgct aaactcggcg atcgcgttga agttacccct ggattgaaaa tccgtatcga
    10741 tggtcacctt atttcggtac gtgagtccgc tgaacaaatt tgtcgcgttc tggcctatta
    10801 caaaccggaa ggtgagttgt gtacccgtaa cgatccggaa gggcgtccaa cggtgtttga
    10861 ccgtctgcca aagctgcgcg gtgcgcgctg gattgccgtg ggtcgtctgg acgttaatac
    10921 ctgcggtctg ctgctgttta ccaccgacgg tgaactggca aaccgcttaa tgcatccaag
    10981 ccgtgaagtt gaacgtgaat atgccgtgcg tgtatttggt caggttgacg acgcgaaact
    11041 gcgtgatttg agtcgtggcg tgcagttgga agatggtccg gcagccttta aaaccatcaa
    11101 gttcagcggc ggcgaaggga tcaaccagtg gtacaacgtt actctgaccg aaggccgtaa
    11161 ccgtgaagtt cgtcgtcttt gggaagcggt tggtgtgcag gtaagccgcc tgatccgtgt
    11221 tcgttacggt gatatcccac tgccgaaagg tctgccacgc ggtggctgga cggagctgga
    11281 cctcgcccag actaactatc tgcgcgaact ggtggagcta ccgccagaaa ccagctctaa
    11341 agtcgctgta gaaaaagacc gtcgtcgtat gaaggcgaat cagattcgtc gagcggttaa
    11401 gcgtcacagt caggtgagcg gcggtcgtcg ttctggtgga cgtaataata a
//
LOCUS       DACBVI010000138        11450 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.139, whole genome shotgun sequence.
ACCESSION   DACBVI010000138 DACBVI010000000
VERSION     DACBVI010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.139"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            389..1078
                     /gene="mutH"
                     /locus_tag="IU325_18085"
     CDS             389..1078
                     /gene="mutH"
                     /locus_tag="IU325_18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HAP5810913.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            1147..1860
                     /locus_tag="IU325_18090"
     CDS             1147..1860
                     /locus_tag="IU325_18090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAP5810914.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            1998..2216
                     /gene="ygdR"
                     /locus_tag="IU325_18095"
     CDS             1998..2216
                     /gene="ygdR"
                     /locus_tag="IU325_18095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HAP5810915.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            2324..3364
                     /locus_tag="IU325_18100"
     CDS             2324..3364
                     /locus_tag="IU325_18100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HAP5810916.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(3396..4589)
                     /gene="lplT"
                     /locus_tag="IU325_18105"
     CDS             complement(3396..4589)
                     /gene="lplT"
                     /locus_tag="IU325_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HAP5810917.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGVVVANIYIPKLVAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(4582..6741)
                     /locus_tag="IU325_18110"
     CDS             complement(4582..6741)
                     /locus_tag="IU325_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-acylglycerophosphoethanolamine
                     acyltransferase/acyl-ACP synthetase"
                     /protein_id="HAP5810918.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAIYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            7326..8357
                     /gene="galR"
                     /locus_tag="IU325_18115"
     CDS             7326..8357
                     /gene="galR"
                     /locus_tag="IU325_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HAP5810919.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(8364..9626)
                     /gene="lysA"
                     /locus_tag="IU325_18120"
     CDS             complement(8364..9626)
                     /gene="lysA"
                     /locus_tag="IU325_18120"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP5810920.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWAYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRLVLEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            9748..10683
                     /locus_tag="IU325_18125"
     CDS             9748..10683
                     /locus_tag="IU325_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP5810921.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAAKEILTPEDFHSENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(10670..11362)
                     /locus_tag="IU325_18130"
     CDS             complement(10670..11362)
                     /locus_tag="IU325_18130"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase"
                     /protein_id="HAP5810922.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRTYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
BASE COUNT         2864 a         3061 c         2835 g         2690 t
ORIGIN      
        1 aaaaaagagt aattcgtgac ccaggtcaca cctctcattt acgggttggc gaaagatcaa
       61 ccaatgcggt atttatccac agaatgtgcc actaagttaa gcactgaacc actaaaaact
      121 ggagtttcgt cgcacgtcaa ggctgtaaat ggaaacagta gtggaggttt ttcacagtta
      181 tcccagcttt ctgtggataa catggtgtaa gatcctgttt attttcagtg accagatttg
      241 gaaaacccgt tgcagtgttg cgcaactcga ttacaggaag cctaaaaagc aatataaatc
      301 agagaattga acaacgcatg tggaaaaagt tacactacga atattcggca cataattgct
      361 gtttattttt taaccaaggt atcgtgacat gtcccaacct cgcccactgc tctctcctcc
      421 cgaaactgaa gaacagttgt tagcgcaagc acagcaactt tctggttata cattgggaga
      481 actggcggca cttgccgggc tggttacacc agagaattta aaacgcgata agggctggat
      541 tggcgtgtta ctggagatct ggctaggtgc cagcgcagga agtaaacctg agcaagattt
      601 tgctgccctg ggcgtggaac ttaaaactat ccctgtggat agtcttggtc gtccgctgga
      661 aacaacattc gtttgtgttg ccccgttaac gggtaatagc ggggtgacct gggaaaccag
      721 ccacgtacgc cacaagctca aacgcgtact gtggataccg gttgaaggcg agcgcagcat
      781 cccgctggcg cagcgtcgcg taggatcacc gttactgtgg agcccgaatg aagaggaaga
      841 ccgacagcta cgcgaagact gggaagaatt aatggatatg attgttctcg gtcaggttga
      901 gcggattacc gctcgtcacg gggaatattt acagatacga ccgaaagcag cgaatgcgaa
      961 agcacttacc gaagccattg gtgcccgggg cgaacggatt ctgacgctgc cacgcggctt
     1021 ttatttgaag aagaatttca ccagtgcact actggcccgt cattttctga tccagtagcc
     1081 atcgctttga cctgccgctt tccgggcata taattaccgc ttcatttttt tggcagggct
     1141 ttttagatgt tatttgcatg gataaccgat cctaacgcct ggcttgcgct cggtacgctg
     1201 acgctgctgg agatcgttct tgggatcgac aatattattt tcctttctct ggtggtggca
     1261 aagcttccca cagcacaacg tgctcatgcg cgccgtctgg ggttggcggg agccatggtt
     1321 atgcgtctgg cgctgctggc atccatcgcc tgggttacgc gcctgacgaa tccgcttttt
     1381 acaatattca gtcaggaaat ttccgcccgt gatttgattc tgcttctggg tggcttgttc
     1441 cttatctgga aagccagcaa ggaaatccac gaatccatcg aaggtgaaga agaagggctg
     1501 aaaacacgcg tttcatcatt cctcggcgct atcgtgcaga ttatgctgct ggatattatc
     1561 ttcagcctcg attcagtgat taccgctgtg ggtctgtcag atcacctgtt tattatgatg
     1621 gcagccgtgg taattgccgt aggcgtgatg atgttcgctg cgcgttcgat tggtgatttt
     1681 gtcgagcgcc atccttcggt aaaaatgctg gcgctctctt tcctgattct ggtgggcttt
     1741 accctgattc tggaaagttt cgacatccac gtaccgaaag gttacatcta cttcgcgatg
     1801 ttcttctcta ttgcggttga aagcctcaac cttattcgca acaaaaagaa cccgctctga
     1861 tactccgtac gctctcctgc gggagggcgt actcactcct gttcccctca cagtttaaga
     1921 ttttcctgct ttcaagatat ataacgtcgg tttataaaca gactattatc ataggtgggc
     1981 caaatgagga taaccggatg aaaaaatggg cagtaataat ttctgcagtc ggactggcgt
     2041 ttgctgtttc cgggtgttcc agtgattacg tcatggcgac caaagatggc cgtatgattt
     2101 tgaccgatgg aaaacctgaa attgatgatg ataccgggct ggtgagttat cacgatcagc
     2161 aaggtaacgc gatgcaaatt aaccgtgatg atgtttcgca aattattgaa cgttaactaa
     2221 taaggtcagc atccggctgg ccttaagatt tttctctttc ccttttccct tccctctgcc
     2281 atttttatat tccttatgtc gtgattataa aaaggaaacg gctatgcaat atcaccgtat
     2341 accccacagt tcgctggaag tcagcacgct ggggcttggc acgatgacgt ttggtgaaca
     2401 gaacagcgaa gccgacgccc acgcacaact cgactatgcc gtcgctcagg gcattaacct
     2461 tatcgacgtt gccgaaatgt acccagtacc tccgcgcccc gaaacgcaag ggttgactga
     2521 aacctacgtc ggcaactggc tggcgaaaca tggcagccgc gaaaagttaa ttatcgcctc
     2581 caaagtgagc ggaccgtcgc gcaataatga caagggcatc cgcccggatc aggcgctgga
     2641 tcggaagaat atccgcgaag cgctgcatga cagcctcaag cgtctgcaga ctgattacct
     2701 cgatctctat caggtgcact ggccgcagcg cccaaccaac tgcttcggca aactcggtta
     2761 tagctggaca gattctgcgc ctgcggtttc gctgctggat acgctggacg cactggcaga
     2821 gtaccaacgc gcgggaaaaa ttcgttatat cggcgtgtcg aacgaaactg catttggcgt
     2881 aatgcgctac ctgcatctgg cggacaaaca cgatctgccg cgtattgtca ccattcagaa
     2941 cccctacagc ctgttaaacc gcagttttga agtaggtctg gcagaagtca gccagtatga
     3001 aggggtcgaa ctgctggcct attcgtgcct gggtttcggc acactgaccg ggaaatatct
     3061 caatggtgca aaacccgctg gcgcacgtaa tacgctcttt agtcgcttca cccgctatag
     3121 cggtgagcaa acgcaaaaag ccgtcgcggc gtatgttgat atcgccagac gtcatggcct
     3181 ggatcctgct cagatggcgc tcgcttttgt acgccgtcaa ccgtttgttg ccagcactct
     3241 gctgggcgca accacgatgg agcagctgaa aactaacgtc gaaagtttgc atctggagtt
     3301 aagcgaagac gtattagctg aaattgaagc ggtacatcag gtttatactt atccggcacc
     3361 ataagattaa cgccggttaa aaccggcgtt aaatattaat gacggcgctg ccaaatccac
     3421 agcgccgtta ttgccagcgc aaacagcgca ccgaagccaa tgccaatggg cacgaccggg
     3481 atgcctacca ttaccgccag cgagtaaatg cccagcatca acaacatggc gctgttttcg
     3541 ccaaggtttt gtactgcaat tgcattcccc gccccgacgc tttttttacc ccgctcctgt
     3601 agcaacgcat tgagcggaac gacaaaaaag ccccccagca cgccaatcag catcaacaag
     3661 gcataggctg gcaacagctc gtgttgcagg gagaaaatca gtaccaccac accaatcaaa
     3721 atcccggctg gcatacagcg tgacacggtt tccagcgtaa ctaacttcgc tgccgcacct
     3781 gcgccaacca cgataccaat cgctaccatc gcgttgagat aggtcggcgt agcgttatcg
     3841 gtaatgccca gcgccaccgg tacccacagc accaacagga aacgcagcgt gacacctgct
     3901 ccccagaata aactggtgcc caccagcgaa aaacgcgttt caccattgcg ccatagcgag
     3961 gtgcaggcat tcaggaaact gcgggtcatg ttgatgagat tccaggactg ccccggacgc
     4021 gccgccacca gtttgggaat gtagatattg gcaacgacca caccaccgta ggccagtgcg
     4081 catgcggcca gggcgaccag gacatgccag tcagccagca caccaccggc tacggaaccg
     4141 agcaaaatcg ccgctatggt agaagcttcc attaaaccgt tagctttcac taacttacta
     4201 cccgtggtta attcgccgag aataccgtat ttcgccggtg aataggctgc agcaccaaca
     4261 cccaccagcg tatagccgag aaacggattg ataccaaagc agatactggc tgcgcccagc
     4321 agcttcaggc cgttggcaaa catcatcacc cggcctttgg cgaagctatc cgccacctgt
     4381 ccgacaaacg gcgcaaaaag aatgtaagca cctacaaaca ccatttgcag gatgggctgg
     4441 ctccactccg gatagaactg cgctttcagt aacgccagag tggcaaacag cagcgcatta
     4501 tcgccaaacg cagagagaaa ctgcgccacg ataaccgctt tcatcccctt cgaccacaac
     4561 gaagtattag tgtgcactga ctcactcatc gtgttgttcc gcttcgtcta cccagctttt
     4621 caacgtgaca aagtcaggtt tgccgctgcc aagtaatggc atctgtttca gatagcgaat
     4681 atcgcgcggt acagcaagct ccggcacgcc gtgctcgcgg gcatactgtt gtaacttatc
     4741 gcgcgtcagt tcgttatctg tggtgaaaag caccagtgcc tcgcctttgc tggcatcgct
     4801 cttaatcgca gtggcatgga ctttatctgg cgaaacacca agtgccagtt gttccaccat
     4861 ttccagcgac accatttcgc ctgcaatttt ggcaaagcgt tttgcgcggc cctgaatctg
     4921 cacaaagccc tgctcgtcaa aacgcacaat atcgccagtg tcataccagc cgcgctccat
     4981 ttcgccgcga acattctcgg cggtgggcac ttccagtaca cctggcttct ccacccgcag
     5041 atagccgttc attatgttcg gccctttcag ttgcaggcgt ccgccctctt cgataccagg
     5101 aaccgacaac aggcgcgcgt ccatacctgg tagaatacgc cctaccgtac cgggtttcgc
     5161 cgccatcggt acgttgatag aaacgacagg cgcgcactcg gtcacaccgt agccttcaag
     5221 gatgcgcagg ccaaatttat cctgccaaag ctgtttggta ctttcttgta gtttttctgc
     5281 gcctgccacc acatagcgta gacgatagaa gtcatacggg ttggcgaaac gcgcgtagtg
     5341 accgaggaaa gtcgaggtac cgaacaacac ggtgcaactg cggtcataca ccaactccgg
     5401 cacaatgcgg taatgcaacg ggcttggata aaggaacact tctgcacctg tgagcagtgg
     5461 cgtaaacagg cctaccgtca gcccaaagga gtgaaacagc ggtaacgccg acataaagcg
     5521 atcgttggtg gtgaagtcgg caatcgtttt aatctgctcg acattcgcca gaatgctttt
     5581 atggctatgg acgacgcctt tcggatggcc ttcagaaccg gaggtaaaaa ggatcagcgc
     5641 ctcttcttcc ggctgctgtt ttacctgtgc cagacgcggc atcagcaaat gagcgaagat
     5701 ccatactttg tcggcagtgg tgacatctgc ttttaaatct tccagataga cccagcgcac
     5761 ctgagtaagt tgctccggca gatgccagag tttgccttta tcgagaaact ggcgggaagt
     5821 gaagatggtt ttgatttcag ccgccgtaat agcactggtc agccctttta ccccggcggt
     5881 gtagttcatc attgcgggaa tgcggcgacg ggcgatggcc ccaaaaatca ctgccgcact
     5941 gatgcctgca ttgggcagca ttaagccgat gcgttcgcct tcaacactgt atttttcaag
     6001 gatgcgtcca acaaacagcg ttttcgtaag caatttgcga taggagtctg gggtaaagtt
     6061 gacgtcttcg acacatttct tcccggctcc gaagcggtat attgcactca gtaaagattc
     6121 gtacagcgtt tcacgcgggc gcaccgccat acgcgcttcc atcattattt gatgcagcat
     6181 ttcgccagcg attttgcgac ggtcacgggc acgcggcgca tccggcatct ccacctgcgt
     6241 tggtggcaaa atatgcagag taatttgcgg gaacaagcga cgtttaacca gacctttcag
     6301 gcggctgaag tgcgtaagtt ccgccccttc aatacgcaca ggaataaccg ttgcaccaga
     6361 cttcgccgcg acaaaacccg caccatcgta gattttcatc agcgagcctg tggtggtgat
     6421 tcgtccttca gggaaaatca ccactggtcg gccctgttcc accagacgta ccagatgttt
     6481 aatcgccata ggttgcgtcg ggtcgagagg aacaaagtcg ataaatgatt tcagccaacg
     6541 catataccac tgttggctta ttgaggtgta aacggcaaat accggacgta cgggtaaaaa
     6601 caatgccagc agtatgccat caataaaaga gacgtgatta ggcgtgatta gaacgcgctc
     6661 gcccttcagt gcctgggtgt cacccgtaac gcgaacgcga tacaaaacac ggcacaaatt
     6721 tcgaaaaaag ctaaaaagca ttccaactcc ctttgctctg attcagtaaa agcgaatgga
     6781 gggagattac acgagataaa gaacgcgagc gacagtaaat taggtgcgaa aaaaaacctg
     6841 cgcatccgcg caggttggtg caagagacag ggtacgaaga gcgtaccgaa taatctcacc
     6901 aatcaatacc tctgggatct tgattgtggt ctgcacgacg actcttcgcc agtgagaaaa
     6961 cgcaaaggaa tgaagggaaa tgcaacgagg tgtgtaaatt gtcggttact gttacagatt
     7021 gatgaccggc aaaaaaaacc tgcgcatctg cgcaggctgg tgtaattcat gtgctcaacc
     7081 cgaagttgac ttcacctatc aatacctctg ggatcaccac tttagcaacc tgaagccaaa
     7141 cgccaccagc ggtcaatcgc aacagcgttt cgcaaagtgt aaccaaaggt ttgcaatctc
     7201 tttttttgtc ttacttattt gcgtttggct cacattccca cgatgaaaac acgccacccc
     7261 ttgaaccaac gggcgttttc cgtaacactg aaagaatgta agcgtttacc cactaaggta
     7321 ttttcatggc gaccataaag gatgtagccc gactggcagg cgtttcagtc gccaccgttt
     7381 cccgcgtcat taataattca cccaaagcca gcgaagcttc ccggcttgct gtgcatagtg
     7441 caatggagtc tcttagctat cacccgaacg ccaacgcccg tgcgctggcg cagcagacca
     7501 ctgaaacggt cggtctggtc gttggtgatg tttccgatcc gtttttcggt gcaatggtga
     7561 aagcggtcga acaggtggct tatcacactg gtaatttttt attgattggc aacggttacc
     7621 acaacgaaca aaaagagcgt caggctattg agcaactgat ccgccatcgc tgtgctgcgt
     7681 tggtcgtcca tgccaaaatg ataccggatg ccgatttagc ctcattaatg aaacaaatgc
     7741 ccggtatggt gctgatcaac cgtatcctgc ctggctttga aaaccgttgt attgctctgg
     7801 acgatcgtta cggtgcctgg ctggcaacgc gtcatttaat tcagcaaggt catacccgca
     7861 ttggttatct gtgttctaac cactctattt ctgacgccga agatcgtctg caagggtatt
     7921 acgatgccct tgctgaaagt ggtattgcgg ccaatgaccg gctggtgaca tttggcgaac
     7981 cagacgaaag cggcggcgaa caggcaatga ccgagctttt gggacgaggc agaaatttca
     8041 ctgcggtagc ctgttataac gattcaatgg cggcgggtgc gatgggcgtt ctcaatgata
     8101 atggtattga tgtaccgggt gagatttcgt taattggctt tgatgatgtg ctggtgtcac
     8161 gctatgtgcg tccgcgcctg accaccgtgc gttacccaat cgtgacgatg gcgacgcagg
     8221 ctgccgaact ggctttggca ctggcggata atcgccctct cccggaaatc actaatgtct
     8281 ttagtccgac gctggtacgt cgccattcag tgtcaactcc gtcgctggag gcaagtcatc
     8341 atgcaaccag cgactaaccg cagttaaagc aattccagtg ccagtaattc ttcgatggtc
     8401 tggcgacggc gaattaaccg cgcctgacca ttatcaaaca gaacttctgg taacagcgga
     8461 cggctattgt agttggatga cattgatgcg ccatatgccc ccgtatcatg caataccaga
     8521 taatcacccg ccttcacttc cggcaaggcg cgggtttcaa catttccccc ttcctgctgg
     8581 gtaaagacat cgcccgattc acataacggc ccggcgacga cggtttccac cgttggtgcg
     8641 tgttccagag aacgaccatc agctgccagg gcactgatat ggtggtaact accgtacatt
     8701 gccgggcgca tcagatcgtt aaacccggca tcaaccagca caaagtggcg gctacccatt
     8761 tgtttgacgc tccgcacctg agtaattaac acgccagact gcgctaccag gaagcgaccc
     8821 ggttcaattt ccagtttcac agggtggccc aaatggcggg cgatttgctc acgcgcggca
     8881 ttccacagac cataataatg ttcggtatca accgcctctt caccctgttg ataaggaata
     8941 gaaagcccac cacccgcaga aatagcctgt aaatcctgac cgaattcgag gaccagacgc
     9001 accatagcac cacatacctg ttccagatgg gcataatcaa cgccagaacc aatgtgcatg
     9061 tgaatgccga ccagctgtag atgatgacgt tgtatcacgt ccagtgcggc gggcagatcg
     9121 gtgtaccaga taccgtgctt gctgttttcg ccacctgtat tggttttttg gctatgcccg
     9181 tgaccaaacc ccggattaac acgcagccat acccgatgcc ctggcgaaac ctgacccagt
     9241 tggtcgagca tatcaacaga acccgcattc accggaattt gcaattcact gacgcgttca
     9301 agcgtcgcct gatcgataac atctgccgta aaaacaatat catcggggtg cgtttgcgga
     9361 ttgtaacccg ccgccaacgc acgctctatt tcgcctaacg agacggaatc cactttcacg
     9421 ccctgctcac gcattaagcg caaaatatga atattggaac aggctttctg tgcgaagcgc
     9481 accacatcaa actgtttcag cgctgcaatc tgccgacgaa taatttgcgc atcgtaggcc
     9541 cacaccgggc agccaaattc tgcgggcaaa cgcagcagat tttcggcggt gaggtcggta
     9601 tcggtgctga acagtgaatg tggcataaca aactccagat aagtgctttt ttatgattac
     9661 gccacatcat aaaaagaata aaaaatatcg atttatgttg agtctatgca aaaatgatat
     9721 ggattaccgg attgcgagag agcgctaatg gccgccgtta acttacgtca tattgaaatt
     9781 tttcatgcgg taatgaccgc cggaagcctg actgaggcgg cacacctgct acacacctca
     9841 cagccaacag tcagccgcga actggcgcgc tttgagaagg tgatcgggct gaaattgttt
     9901 gagcgcgtac gtggacgatt acatcctacc gtgcaaggac tgcgtctgtt tgaagaagtg
     9961 caacgatcct ggtacggact ggatcgcatt gtcagcgccg cagaaagtct gcgcgagttt
    10021 cgccagggag aactgtctat agcctgcctg ccggtctttt cgcaatcttt tttaccgcag
    10081 ctcctgcaac cctttctggc acgttatccc gatgtcagct taaatatcgt gccccaggaa
    10141 tcacctctac ttgaagagtg gctctcggcc cagcgtcatg atttagggct cactgagacg
    10201 ctccatacgc ctgcgggaac agaacgtacc gaattactct ctttagatga agtgtgtgtg
    10261 ttacctccgg gtcatccgct ggcggcaaaa gagatattaa cgccagaaga ttttcacagt
    10321 gagaactaca tcagcctttc ccgtactgac agctatcgcc agttgctgga tcaattgttt
    10381 actgaacatc aggttaaacg acgcatgatc gtagaaaccc acagcgccgc gtcagtctgc
    10441 gcaatggtac gggcgggggt aggtgtttcg gtggttaacc cgctcaccgc actggattat
    10501 gcggcaagcg gtttagtggt gcggcggttc agtattgcgg ttccgttcac cgtcagcctg
    10561 atccgccccc tgcaccgccc gtcatcagcg ctggttcagg cgtttagtga gcatttacaa
    10621 gcgggattac cgaaactggt cacttctctt gacgctattt tgtcgtcagc tacgacagca
    10681 taaaagcgac agcatcctcg gcatggatcg ccgcggtatc aaacacaggc agaacactgc
    10741 gctcttctgg caccagtaaa ccaatttccg tacagccaaa aatgacgccc tgtgcgccct
    10801 gttctgcaag gcgagcaatc acttgcgcat aataagtgcg tgacgcttcg gtaaattgcc
    10861 ccagacacag ttcttcaaaa ataatctgat taattttcgc ccgttcatcc gcttcaggaa
    10921 tgagacagtt gatggaaaat tgttccgtca gccgcccgcg ataaaaatcc tgttccatgg
    10981 tgtaacgcgt acccagcagc gccacacgag tcatcccggc cccggtaatt gcacgtccgg
    11041 tggcatccgc aatgtgtaag aaaggcagag agcaacgaga ctcaatggca tccgccactt
    11101 tgtgcatcgt attggtacat aacacaatgc cttctgcgcc cgcccgctgt aagccaagcg
    11161 ccgcctcagc caaaatgtcc ccggttttgt cccactcgcc gcgacgctgg cactcttcta
    11221 tttcatgaaa atcgacgcta tgtagcagca cttgcgcaga gtgaagccca ccaagccgct
    11281 gtttaatacc ttcattaatc agacgataat aaggaatagt ggattcccaa ctcattcctc
    11341 ccagcaaacc aattgttttc attcctctct cctgatgtgt gttaccgcaa caaggctgaa
    11401 taaggaacga gacaaacgcc tcaacggcca ggtgcccaat ctctattaac
//
LOCUS       DACBVI010000139        11380 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.140, whole genome shotgun sequence.
ACCESSION   DACBVI010000139 DACBVI010000000
VERSION     DACBVI010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.140"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            26..931
                     /locus_tag="IU325_18135"
     CDS             26..931
                     /locus_tag="IU325_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP5810923.1"
                     /translation="MGGKIPKTELLVTFEVVARHESYTRAAEELALTQSAVFRQVSAL
                     EEFLHTPLFSHSKKRIFLNDAGKYYLGIVKETLNKLERDTNTIMTWQPTVKVIELAVN
                     PTFSTHWLIPNLHEFTKLHPDIIVNIHSLANNGDFLNREYDAVIMRENFCAPWAEVEY
                     LFDEEILPVCSGSLLAMSDQKLSVAELLTELPLLHQSTRITGWEEWFALSGVSSPLVN
                     NGPRFDLLSMLIAAVRSNLGVALLPRFAIQHDLDSGDMVIPCDVPIRTGNRFIMTWQE
                     EKSDSPHLQQFREWLLAKSVVPQAM"
     gene            complement(965..1567)
                     /gene="cobO"
                     /locus_tag="IU325_18140"
     CDS             complement(965..1567)
                     /gene="cobO"
                     /locus_tag="IU325_18140"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308783.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HAP5810924.1"
                     /translation="MEARISTERHVQRQQKLKEQVDTRVAAAIEKKGILIVFTGNGKG
                     KSTAAFGTVTRAVGHGKTVGVAQYIKGQWDNGEYNLLQPLGVEFHIMGTGFTWETQNR
                     QADIDAAKEVWSESKRMLADKRYDLVVLDELTYMLAYHYMDTEEVIASLQNRPAQQSV
                     IVTGRGCHSQILKMADTVSEIRPVKHAFDNGIQAQPGIDW"
     gene            complement(1577..3229)
                     /locus_tag="IU325_18145"
     CDS             complement(1577..3229)
                     /locus_tag="IU325_18145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HAP5810925.1"
                     /translation="MSKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPE
                     ALTLLARQAFYEASFFLRSAHLQQVASILNDPQASSNDKYVALQLLRNAEVSAKGVLP
                     NCQDTGTATIVASKGQQIWTGGNDAEALSKGIYSTFQENNLRFSQNAPLDMYTEVNTQ
                     TNLPAQIDISAVAGNEYHFLCVNKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSL
                     GTAACPPYHIAFVVGGLSADQTLKVAKLASTKYYDNLPTSGNEQGQAFRDIELEKVLL
                     EASQQFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSADRNIKAKINKHGIWL
                     EKLEHNPGQYIPASLREENHAQHVQLDLNRPLRDVMQDLARLPVGTRVSLSGPIVVAR
                     DIAHAKIKARLDSGEPMPDYLKHHIVYYAGPAKTPENMACGSLGPTTGGRMDGYVDTF
                     QAAGGSLVMLSKGNRSQQVTDACHKHGGFNLGSIGGAAALLAQEYVKSLRCLEYPELG
                     MEAVWMMEVENLPAFILVDDKGNNFFSQFEQQHRCASCPAGH"
     gene            complement(3337..4617)
                     /locus_tag="IU325_18150"
     CDS             complement(3337..4617)
                     /locus_tag="IU325_18150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308785.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HAP5810926.1"
                     /translation="MKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFT
                     TIFLRLIKMIIAPLVVSTLVVGIAKMGDAKALGRIFSKTLFLFICASLLSIALGLITV
                     NFFMPGTGINFVAHGAETTGVVASEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSI
                     FLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVM
                     VSAGIFMGEFYFTMLLLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFP
                     GTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSFATVFIAQACNIHLSIGEQITM
                     LLILMLTSKGMAGVPRASMVVIAATLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSN
                     AMGAAMVSRWEGEHFGEGCRGKALKPNESNVALP"
     gene            complement(4778..5095)
                     /locus_tag="IU325_18155"
     CDS             complement(4778..5095)
                     /locus_tag="IU325_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 family protein"
                     /protein_id="HAP5810927.1"
                     /translation="MKQSIVSLAQVIRSKNAGPYELVLDILFKTKENYERVKSSGQLT
                     PELIARLYHVEPDFIHRIVWFDPSNAVKIVMPRDIISGNVGDNDVYGAQQHAPLLNMS
                     FDL"
     gene            complement(5092..6462)
                     /locus_tag="IU325_18160"
     CDS             complement(5092..6462)
                     /locus_tag="IU325_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HAP5810928.1"
                     /translation="MARSFKVLSPTAILGYGFPEESFRKAMAESPDLIAVDAGSSDPG
                     PHYLGAGKPFTDRAGVKRDLRYMITAGVQNNIPVVIGTAGGSGAAPHLEWCREIIHEI
                     AQEEHLSFSMALIPADVDKAIVHQALDNGKITALEFVPPLTHDAIDESTYIVAQMGIE
                     PFQRALKEGAQVVLGGRAYDPACFAALPIMQGFDEGLALHCGKILECAAIAATPGSGS
                     DCAMGIIDDNGFTLKTFNPKRKFTETSAAAHTLYEKSDPYFLPGPGGVLNLKGCTFKA
                     VNDSEVYVSGSKHEETPYALKLEGARRVGFRCLTIAGTRDPIMIAGIDKIIDEVKTSV
                     SRNLSLDDDSIRINFHLYGKNGVMGDHEPMQTAGHELGIVLDVVAPTQEIANSVCSLV
                     RSTMLHYGYENRIATAGNLAFPFSPSDIQGGPVYEFSIYHLIEANDALRFDFHIEQVT
                     PEGVQA"
     gene            complement(6466..7707)
                     /locus_tag="IU325_18165"
     CDS             complement(6466..7707)
                     /locus_tag="IU325_18165"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HAP5810929.1"
                     /translation="MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFFYTGEPVTQGFN
                     AVRQAGECVSVQLILENGAVAVGDCAAVQYSGAGGRDPLFLAEHFIPFLNDHIKPLLV
                     GRDVDAFLPNARLFDKLRIDGNLLHTAVRYGLSQALLDATALATGRLKTEVVCDEWQL
                     PRVAESIPLFGQSGDDRYIAVDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWL
                     SDRILSKRTSARYHPTLHIDVYGTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPV
                     DAGNKPDQIRLLTAITKELTRLGSGVKIVADEWCNTYQDIVDFTDAASCHMVQIKTPD
                     LGSIHNIVDAVLYCNSHSMEAYQGGTCNETDVSARTCVHVALAARPMRMLVKPGMGFD
                     EGLDIVFNEMNRTIALLQAKD"
     gene            complement(7707..9152)
                     /locus_tag="IU325_18170"
     CDS             complement(7707..9152)
                     /locus_tag="IU325_18170"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HAP5810930.1"
                     /translation="MELRNKKLTHDEFMTERQQVLKTWETGKDVENFEDGVKYQQTIP
                     EHKRFSLALLKADKEGKTLSQPRAGVALMDEHIELLKTLQEECDLLPSTIDAYTRLNR
                     YEEAALGIKKSIEAGTSKLNGLPVVNHGVAACRRLTEALQKPLQIRHGTPDARLLAEI
                     SMSSGFTSYEGGGISYNIPYAKRVTLEKSIRDWQYCDRLMGMYEEHGIRINREPFGPL
                     TGTLIPPFISHSIAIIEGLLALEQGVKSITVGYGQVGSLTQDVAAIQSLRELAHEYFQ
                     SYGYTDYELSTVFHQWMGGFPEDESKAFAIISWGAAVAGMSGATKVITKSPHEACGIP
                     TAAANIQGLKASRQMLNMVNEQKFPPCPAVELEIELIKSEVRAVLNKVFELGNGDIAR
                     GTVLAFEAGVLDVPFAPAACNAGKILPVRDNTGAIRVLEAGAVPLPKDILDLHHDYVA
                     ERARFEGRQPTFQMVVDDINAVSHSKLIGRP"
     gene            complement(9171..10559)
                     /locus_tag="IU325_18175"
     CDS             complement(9171..10559)
                     /locus_tag="IU325_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase"
                     /protein_id="HAP5810931.1"
                     /translation="MQIVSVDIGSTWTKAALFTREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLDQVLNVDDALPLLNSGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQYYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPLAARQAICDIFLKRIVKGKGLDV
                     IVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSACANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAMVVGESAKELVSVNFAKQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            complement(10559..11008)
                     /locus_tag="IU325_18180"
     CDS             complement(10559..11008)
                     /locus_tag="IU325_18180"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HAP5810932.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRECCIERGLGNILLYVGGNLVVGKHDFA
                     DVETKFKEMGFDRVFSPSHDLEDVCQLMAHDINQRHNVDTRILEEAI"
BASE COUNT         2750 a         2837 c         2819 g         2974 t
ORIGIN      
        1 taaactattt ttaagggacg gcattatggg aggaaaaata cctaaaacgg aactgctggt
       61 gacttttgag gtcgtcgcac gtcatgaaag ttataccaga gcggcagagg agttagcgtt
      121 gacccaaagt gcagtattca gacaagtgag tgcgctggag gaatttttac atactccgtt
      181 attttcacac tctaaaaaac gtatttttct taatgatgca gggaagtatt acctggggat
      241 agtaaaagaa acattaaata agctggaacg cgataccaat accattatga cctggcaacc
      301 tacagttaag gttattgaac ttgccgtgaa tcctacgttt agtacccact ggttaatccc
      361 aaatttacat gaattcacaa agttacatcc cgatattatt gttaatattc actctttagc
      421 taataacggc gattttctta atcgggagta cgatgctgtc attatgcgcg aaaatttttg
      481 cgcgccctgg gcggaagtag aatatctctt tgatgaggag attttgccgg tatgcagcgg
      541 cagtttgctt gcaatgtccg atcaaaaact gtcagtggca gaactgctta cagagttacc
      601 acttctgcat caaagtaccc gtattactgg ctgggaagag tggtttgcct tatctggcgt
      661 tagcagtccg ctggtcaaca acggaccacg tttcgatttg ctttcaatgc tgattgccgc
      721 cgtgcgctct aatctcggcg tggcgctgtt accacggttc gctattcaac atgatttgga
      781 tagtggcgat atggtgattc cctgtgatgt gcctattcgt accggtaatc gttttattat
      841 gacgtggcag gaagagaagt ctgactcgcc gcacttgcag caatttcgtg aatggttgct
      901 ggcgaaatcg gttgtaccgc aggcgatgta aaacctgcgc ctggcgggat gccaggcgca
      961 gttattacca gtcgataccc ggctgcgcct gaatgccgtt atcaaaagca tgtttgacgg
     1021 ggcggatttc gctgaccgta tccgccattt ttaatatctg actgtggcaa ccgcgtccgg
     1081 tgacaatgac actttgttgt gccgggcggt tttgcagaga agcgatcact tcttcggtat
     1141 ccatataatg gtaagccagc atataggtca gctcatcaag caccactaag tcgtaacgct
     1201 tgtcagccag catccgtttg ctttccgacc agacctcttt cgcagcgtcg atatccgctt
     1261 gccggttctg tgtctcccag gtaaaaccgg ttcccataat atgaaactca accccgagcg
     1321 gctggagaag gttgtattcg ccgttgtccc attgcccctt gatgtactgc gcaaccccca
     1381 ccgttttgcc atgacccacc gcgcgcgtca cagtaccaaa cgcggcagtg gatttccctt
     1441 tgccattgcc ggtgaaaaca atcaaaatcc cttttttctc tatcgccgcc gcgacgcgcg
     1501 tatcgacctg ttccttcaat ttctgctggc gttgcacatg ccgttcagtt gaaattcgcg
     1561 cttccattca ttttccttaa tgtcccgcag ggcaactggc gcagcggtgt tgctgctcaa
     1621 actgactaaa gaaattattg cctttatcat ctaccaggat gaatgcgggc agattttcca
     1681 cttccatcat ccacaccgct tccataccca gctccgggta ttccagacaa cgcagacttt
     1741 tgacatattc ttgcgccagt aacgcagctg caccgccaat actacccaga ttgaagccac
     1801 catgtttgtg acaggcatcg gtgacctgct ggctgcggtt acctttcgat aacattacca
     1861 gactgccgcc agcagcctgg aaggtatcga cataaccgtc cattcgaccg cccgtggttg
     1921 gacccagtga accgcaggcc atgttctccg gtgttttcgc cgggccagcg taatagacaa
     1981 tatgatgctt caggtaatcc ggcatcggtt cgccgctatc cagccgcgct tttattttgg
     2041 cgtgggcgat atcgcgcgcc actacgatgg gaccacttaa cgacacgcgg gtacctaccg
     2101 gtaatctggc gagatcctgc atcacgtcac gcaacggacg gtttagatcc agttgtacat
     2161 gttgcgcgtg attttcttcc cgcagtgagg ctggaatata ttgccccgga ttgtgttcga
     2221 gtttttccag ccagataccg tgcttgttaa tttttgcttt gatattgcga tcggcagaac
     2281 aggagagcgc catagctatc gggcaggagc caccgtgacg cggcagacga atgacgcgaa
     2341 tatcatgagc gaaatattta cctccaaatt gcgcaccaat accaaattgc tgactggcct
     2401 cgagcaatac tttttccagc tcaatatcac ggaatgcttg tccttgctcg tttccgctgg
     2461 tgggcaaatt gtcgtaatat ttcgtagagg ccagcttggc aacttttaag gtttgatcgg
     2521 ctgaaagccc tccgacgaca aaggcgatgt ggtaaggcgg gcaggcagcg gtgcccagcg
     2581 atttcatttt ttcgattaaa aatgcggtca gtttttctgg ttgcaataag gatttggttt
     2641 cctgataaag tgcggcttta ttggcggagc cgcccccttt attaacacag agaaaatggt
     2701 actcattacc agcgacagcg ctgatatcaa tctgcgccgg gagattggtt tgcgtattga
     2761 cttcggtata catatccagc ggcgcatttt gtgaaaagcg cagattattt tcctggaaag
     2821 tactgtaaat gcctttgctt aaggcttcag cgtcgttacc tcccgtccat atctgttgcc
     2881 ctttactggc aacaatagtt gcggtgccgg tatcctgaca gttgggtaat accccttttg
     2941 ctgagacttc agcattacgt aataactgca acgcaacgta tttatcatta ctactggcct
     3001 gcggatcgtt aagaatgctg gcgacctgtt gcaaatgagc ggaacgtaag aaaaaagagg
     3061 cttcataaaa tgcctgccgg gcaagcagcg ttaatgcttc aggagcgatt tttatcactt
     3121 cttcgccgtc taattccgtt actgtaatat gttgatcgct aattaatgta tattctgtac
     3181 cgtctttgtt ttgtagaaac ggttcttgcc agataaatgg tttggacata tttcactttt
     3241 caccttaaac gttagtttta cttaattgcc gtctaacttc atgatccggc ttataaaaat
     3301 gcgtagtcat tggcttctga ttgcttaaga tccccctcag ggcagagcaa cgttcgattc
     3361 attgggtttt aatgctttac cccgacagcc ctcgccgaaa tgttcgcctt cccaccgact
     3421 caccatcgca gcgcccattg cgttgctcat gacgtttgtc gctgaacgcc ccatatcaag
     3481 gaacggatca acgcccatca gcaagatcag ccccgcttcc ggaatattga actggttgag
     3541 cgtggcggcg ataaccacca ttgaggcgcg tggtacgcca gccattcctt tcgaggtcaa
     3601 catcaggatc aacagcatgg tgatttgctc accgatggat aaatggatat tgcaggcctg
     3661 ggcgatgaaa actgtggcga aggagcagta ggccattgat ccaacgagat taaatgagta
     3721 gccaatgggt aagacaaagc tggcaatttt gggggaaacg ccaaattgct ccagtttttc
     3781 aagcgttccc ggaaaagccg cttcagaact ggatgtggta aatgccagca gggcgggttc
     3841 cgaaagggcg cgggtcaggc gtctgatgca ggggccaaca taaacgatgg ccagaccgat
     3901 aagcagcacc caaagtaata acatggtgaa ataaaattca cccataaaga tcccggcgct
     3961 caccataact gccagtcctc gttcagcaat caatgctgaa atagcagcga atacggtcag
     4021 gggagcgaag agcatgacgt agccagtgag ctttaacatg gcatgtgcca gcgaatctaa
     4081 ggcgtgaacg atggcgctgc ctttctcgcc aatcgccgtc aggctacagc cgaggaaaat
     4141 tgagaacacc acgatttgca aaatttcatt gtgcgccatg gcatcgacaa tgctggtggg
     4201 gaaagcatgc gaaataaata cttttagcgt aaagggttct gacgcgacca ctccggtggt
     4261 ttcggctccg tgtgcaacaa aattaattcc tgtgcctggc atgaagaaat ttactgttat
     4321 caagcctaag gcaattgaca gcaatgaggc gcaaataaat aaaaagagtg ttttagaaaa
     4381 aatacgacca agggctttgg catctcccat tttagctata cctaccacca gggtagagac
     4441 cactaacgga gcgataatca tttttatcag tcgtaagaaa atcgtcgtga atatcgatat
     4501 ttcttgcgca tagagttttg cggtttccgg tgaagcagtg ttattgagca ctacgccgat
     4561 aatcattcca agtaccagcg ccaaaagaat catcgtggtt aaacttattt tcttcatttt
     4621 ttcctccatc aggtgcaccg cgataaatca gaatcaggag aagtagatgc ccgaatagag
     4681 attcaggcat ctacctgcta ttacaacctt acaaatagct gaagtgttga actgcaataa
     4741 gtgacgggat gtcagttggc taaaagtgtt cggattttta caaatcgaaa ctcatattta
     4801 ataaaggtgc atgttgctgt gcgccataga catcattgtc acctacattc ccggagataa
     4861 tatctcgtgg catgacgatt tttactgcgt tagaaggatc aaaccagaca atacgatgaa
     4921 taaagtcagg ttcaacatga tataagcgcg cgatcagttc tggggttaat tgccctgatg
     4981 atttaactcg ctcatagttt tctttcgttt taaataaaat atctaaaacc agttcgtaag
     5041 gtccggcatt tttcgaacga atgacttgcg ccagtgagac aattgactgt ttcatgcctg
     5101 aactccttct ggcgtcacct gttcaatatg gaaatcaaaa cgcagagcgt cgttggcttc
     5161 aatcaggtga tatatggaaa attcgtatac tggcccgccc tggatatcag aaggggagaa
     5221 cgggaacgcc agattacctg cggtagcgat gcggttttca tagccgtagt gcagcatggt
     5281 agagcgcacc agcgagcaaa cgctgttggc aatttcctgg gtcggtgcaa ctacatcaag
     5341 gacaatcccc agctcatgcc cggcagtttg catcggttca tggtctccca tcaccccgtt
     5401 tttgccgtac aggtggaaat tgatgcgaat gctgtcatcg tcgagcgaca ggttacgcga
     5461 aacgctggtt ttgacttcat cgatgatttt atcgatcccg gcgatcatga tcgggtcgcg
     5521 cgttcctgcg atggtcagac agcggaagcc cactcgtcgc gcaccttcca gtttcagggc
     5581 atacggcgtt tcttcatgct tagaaccgct gacgtacact tcgctgtcat tgaccgcttt
     5641 aaatgtgcag cctttcaggt tcaacacgcc gccaggtcca ggcaggaagt agggatcgga
     5701 tttctcatac agtgtgtgtg cagccgctga cgtttcggta aatttacgct tcggattaaa
     5761 tgtcttcagc gtaaagccgt tgtcatcaat gatgcccatc gcacagtcag agcctgagcc
     5821 gggcgttgcg gcgattgccg cgcattcaag gattttccca caatgcagcg ccagaccttc
     5881 atcaaagcct tgcatgatcg gaagcgcagc aaagcaggcc gggtcgtaag cgcgcccgcc
     5941 cagcaccact tgcgcgcctt ctttcagcgc ccgctggaag ggttcgatgc ccatttgcgc
     6001 aacgatatac gtactttcgt caatcgcgtc gtgggttaac ggcgggacaa actccagcgc
     6061 cgtgattttg ccgttatcca gcgcctggtg gacgatggct ttgtcaacgt ctgccgggat
     6121 cagcgccatt gagaacgaga ggtgctcttc ctgggcaatc tcatgaatta tttctcggca
     6181 ccactccagg tgtggtgcgg caccagaacc gccagccgtt ccgatcacca ccggtatatt
     6241 attttgcacg cctgcggtga tcatatagcg cagatcgcgc ttgacccctg cgcgatcggt
     6301 aaacggtttg cctgccccga ggtagtgggg gcctggatcg gaagagcctg catcaacggc
     6361 gatcagatcg ggcgattctg ccatcgcttt acggaagctc tcttccggga atccataccc
     6421 gaggattgcc gttggcgata acactttaaa agaacgagcc atttgttaat ccttagcctg
     6481 caacagcgcg atagtacgat tcatttcgtt gaagacgata tcgaggcctt cgtcaaagcc
     6541 catccctggc tttaccagca tacgcatagg gcgagcggca agggcgacgt ggacacaggt
     6601 gcgggcactg acatcagttt cattgcaggt gccgccctgg tacgcttcca tgctgtggct
     6661 attgcagtaa agaaccgcat cgacgatgtt gtgaatactg cccagatccg gggttttgat
     6721 ttgcaccatg tgacaactgg cggcatcagt aaaatcaaca atatcctggt aggtgttaca
     6781 ccattcatcg gccacaattt tcacgccgga accgaggcgc gtcagctctt tggtaatcgc
     6841 ggtcagcagg cgaatttgat cgggcttgtt accggcatcg accggccctt caatgtagag
     6901 cggcaggcct tgcgcttctt tttccaggct ggcgatgtat tgcgcacagc gaagcggatc
     6961 catatcgaag atcagaccga tagtgccgta tacatcgatg tgcagggtag ggtggtagcg
     7021 tgcgctggtg cgcttgctca gaatacgatc cgacagccag cggacatatt cgcgcagttt
     7081 ttcaccttta aagcctagtt tctcttcgac gttattaatc agtgcatggg gcagcacgtc
     7141 gatgccctta aggatcatct tatcgacggc gatatatcga tcgtcaccgc tctgaccaaa
     7201 taatggaatg gattccgcca cgcgtggcaa ctgccattca tcgcaaacta cttcagtttt
     7261 cagacggccg gttgccagcg cggtagcatc aagcagcgcc tgtgataatc cgtagcgcac
     7321 ggcggtatgc agcaagttgc cgtcaatacg caatttgtcg aataaacggg cattcggcag
     7381 gaaagcatcc acatcgcggc ctaccagtaa tggcttgata tggtcgttga gaaacggaat
     7441 aaagtgctca gcgaggaaca gtggatcgcg accacctgcc ccggaatact gtacggcagc
     7501 acagtcacct acggcgaccg cgccgttttc cagaatcaac tgtaccgata cgcactcccc
     7561 ggcctgacga acggcgttaa acccctgtgt taccggctcc ccggtataaa aaaagccgtc
     7621 atgacccgct ccgtttttta tcgcctgctg gtcatcgaaa tagaatgagg agtagccagc
     7681 ggtgaacagg gcctgtttta ttttcattat ggtcttccta ttaatttact gtgggataca
     7741 gcattgatgt catctacaac catctggaat gtcggttggc gtccttcaaa gcgtgcgcgt
     7801 tcagcaacgt aatcgtggtg gaggtcgaga atatcttttg gcaatggaac ggctccagct
     7861 tccagtacgc gtatcgcgcc ggtgttgtca cggaccggaa gaattttgcc cgcgttacac
     7921 gcggctggtg caaacggcac atccagtacg cccgcttcaa atgccagcac cgtaccgcgc
     7981 gcaatatcac cattacccag ttcgaacact ttattgagta ccgcgcgaac ctcgctctta
     8041 atcagttcaa tttccagttc aaccgcaggg cacggcggga atttctgctc attgaccatg
     8101 ttgagcatct ggcgggaggc tttcagcccc tgaatgttgg cagcggcagt cgggattcca
     8161 caggcttcgt gcggactttt ggtgatgact ttggtggcac cagacatccc cgcgacggct
     8221 gcgccccagg agataatagc gaacgctttc gattcgtctt ccggaaagcc gcccatccac
     8281 tggtgaaaca cggtacttaa ttcatagtcg gtatagccat agctctgaaa atattcgtgt
     8341 gcaagttcac gcagagactg gatcgccgca acatcctgcg tcaggctgcc gacctgtcca
     8401 taaccaacgg tgatagattt cacaccctgt tccagcgcca gcaagccttc gattatcgcg
     8461 atagagtgcg agataaacgg cgggatcagc gtacctgtta gtgggccaaa cggttcgcgg
     8521 ttgatgcgga tcccgtgttc ttcatacatc cccattaaac ggtcgcagta ctgccagtcg
     8581 cggatcgatt tttccagcgt tacgcgtttg gcatacggaa tgttgtagga aatgccgccg
     8641 ccttcataac tggtaaagcc gctggacatc gagatttctg ccagcagacg cgcatctggc
     8701 gtgccgtggc ggatctgtag cggtttttgc agggcttcgg tgaggcgacg gcaagctgcg
     8761 acaccatggt taactaccgg caaaccgttc agtttagagg ttccggcttc gatggatttt
     8821 ttaatcccta aagccgcttc ttcgtaccga ttcagacggg tatatgcatc gatagtgctc
     8881 ggcagcagat cgcactcttc ttgtagtgtt ttcagtagtt cgatgtgttc gtccatcagc
     8941 gccacacctg cgcgcggttg gctaagggtt ttaccttcct tgtctgcttt caacagcgca
     9001 agagagaagc gtttatgctc aggaatggtt tgttggtatt taacgccatc ttcaaagttt
     9061 tcaacatcct tgccggtttc ccaggttttc agtacctgtt gccgctcggt catgaattcg
     9121 tcatgggtta atttcttatt tcgaagttcc attgattgca gccttaattg ttatagggtt
     9181 aagcattgaa tactggtgcg cgcggcggcc agtggatcga ctctggcgac gtttgccagc
     9241 agtggtaata gcccccggga atcgcggtaa tattcaaatt cggtcggtaa gagaatgcgt
     9301 ttaccgtcgt cgtttaactc gcggtatttc aaccagtgat gcatatcgaa ctgactggca
     9361 cgcgagagcc aaccgccgga acccacgact ttacgcacgg ttgtcaggtc acgtcccatc
     9421 tgtaaatcga cattgccgac gcaggtgcag acctcttttt tggtgcccgc atgtctttcg
     9481 gtggcataac ccacacacag tccggccagg acggtatcga aatatttttc ttctgcattc
     9541 gcgggtaagt agtcgggatg agcgactaga tgacggaggt aatgataaaa agcgtccaac
     9601 tgtgcgggtt gcttagcaaa attcacactg actaactctt tcgcactttc accaaccacc
     9661 attgccgaga cgcgcattcc cagatcgcct tccaccgtcc gttttacgaa gggttccggt
     9721 acgccatgta aaacagtgtc gggcgagagc gtgttggcac aggcggaata gacatcggtg
     9781 gttgcaccgc ccatgtcgat cagcatgaac tctttccagc tgtgatcgac gttactgatt
     9841 gccttaacca gttcaaagac cgtccatggt gtcggcattg gttcttcgcc tgttttatca
     9901 acaatgacat ccagcccttt ccctttaacg atgcgtttaa ggaatatgtc gcagatagcc
     9961 tggcgtgcag ctaaagggtt cggatggtca agatcgggca gaacgttgtc gacgatggtc
    10021 aggtctttat gcccgaggat ctcctgcacc tcgtcctgaa tatcccggtt tccggcataa
    10081 ataatggcgc agtcgagttt ggattctgcc aacacacggg cgttattcag tccgtagctt
    10141 tcttctccgc catcggtgcc gccggtgaat aagagaatat cgggctgcgt ttcttccagt
    10201 gcctggatat cacggcggtt cagcttgtag gcgtaatatt gcgcaatttt agcacccgca
    10261 gagtgagcgg ttactttcgc ggtttccagg gtgatagaag gcaccaatcc cattgcggca
    10321 accgccagcc cacctttggc agaggaggaa tacttcaatg caacctcacc actgtttaac
    10381 agggggaggg catcatctac gtttaacacc tggtccaggc tggagaaaaa ccccttcgcc
    10441 agatgatggg tggtggttgg ggtcagaacg tggttaacca acgttaacgc gtccccctcc
    10501 cgggtgaaga gggctgcttt ggtccacgtc gaaccaatat cgacagagac gatttgcatc
    10561 agattgcctc ttcaagaatg cgagtatcaa cgttatgacg ttggttgata tcgtgggcca
    10621 tcagctgaca aacatcttct aagtcatgac ttggggaaaa gacgcgatca aatcccatct
    10681 ctttgaactt ggtttccacg tcagcgaagt catgttttcc gaccaccagg ttaccgccca
    10741 catagagcag gatgttaccc agcccacgct cgatgcagca ttcgcgcatg cccagacaat
    10801 cgatatcgcc atgcccatag atagaggaga cgacaatcgc gtcagcacca gtttcaatgg
    10861 cagcatcaat atattcgtcc tggctgacca ttacgcccag attaataact ctgaagtcat
    10921 ggttgctaaa aacgcggtcc agaactttat tgcctactgc atggcagtcc gcgccaataa
    10981 cgccaatcac aagtgttgct ttcttcatgg gtaatccttt gtaagacaga gagtaaaata
    11041 agttacacac gtcgtattta tggccgctca taacggcaca ggtttaatat cacaggagtg
    11101 tctttatctg accgtaatgt tgcaattgat ctattttcaa aatgtctttc gggaaatgat
    11161 tgtttaaagt gtgttttcaa attctcattt taattcagtg tgttatgtca actaatgtca
    11221 tgaatgttaa atttttaaaa ttaacttgat ttgatgtatt gagataattg tgatcaactt
    11281 ctaatttgca tttttgtgac gtggctttag ccatgagtaa atgcatttca taaaagttaa
    11341 gtcgaatttc actgcaattc atctctgctt aatgtaaaaa
//
LOCUS       DACBVI010000140        11325 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.141, whole genome shotgun sequence.
ACCESSION   DACBVI010000140 DACBVI010000000
VERSION     DACBVI010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.141"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            220..720
                     /gene="tssB"
                     /locus_tag="IU325_18185"
     CDS             220..720
                     /gene="tssB"
                     /locus_tag="IU325_18185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HAP5810933.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSGK"
     gene            755..979
                     /locus_tag="IU325_18190"
     CDS             755..979
                     /locus_tag="IU325_18190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810934.1"
                     /translation="MAAHKNISGKHPLSDEDILQAVYRVCFSPSGEINRDGVDALITT
                     LDRRISDLLDEIIHLCEFQQSAPYWRRVLH"
     gene            1030..2505
                     /gene="tssC"
                     /locus_tag="IU325_18195"
     CDS             1030..2505
                     /gene="tssC"
                     /locus_tag="IU325_18195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HAP5810935.1"
                     /translation="MSLQEEELVSSHAGQTDQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2512..2925
                     /gene="tssE"
                     /locus_tag="IU325_18200"
     CDS             2512..2925
                     /gene="tssE"
                     /locus_tag="IU325_18200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HAP5810936.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIAQCIERYEPRISRAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2929..4779
                     /gene="tssF"
                     /locus_tag="IU325_18205"
     CDS             2929..4779
                     /gene="tssF"
                     /locus_tag="IU325_18205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HAP5810937.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAESFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPEHYDVFSVNGVISQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERTGQHPLI"
     gene            4743..5825
                     /gene="tssG"
                     /locus_tag="IU325_18210"
     CDS             4743..5825
                     /gene="tssG"
                     /locus_tag="IU325_18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HAP5810938.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAQGEKGTPELSLRDDPAQETLRFSADASLAFPLSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSDVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5850..7130
                     /gene="tagH"
                     /locus_tag="IU325_18215"
     CDS             5850..7130
                     /gene="tagH"
                     /locus_tag="IU325_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HAP5810939.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSTDPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDFPVAEMAQRHLAVTPLLRGLGGSLTVSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNAR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQIYDRVLREKQREPEA"
     gene            7127..7651
                     /gene="tssJ"
                     /locus_tag="IU325_18220"
     CDS             7127..7651
                     /gene="tssJ"
                     /locus_tag="IU325_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HAP5810940.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7654..8985
                     /gene="tssK"
                     /locus_tag="IU325_18225"
     CDS             7654..8985
                     /gene="tssK"
                     /locus_tag="IU325_18225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HAP5810941.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQHFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            8990..9751
                     /locus_tag="IU325_18230"
     CDS             8990..9751
                     /locus_tag="IU325_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HAP5810942.1"
                     /translation="MDEGSLSLPPFTGHHEKSQRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9760..>11325
                     /locus_tag="IU325_18235"
     CDS             9760..>11325
                     /locus_tag="IU325_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP5810943.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKVAGTLTDAADTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTNTSRMTEVLVQYDETLTALDELEAAWHQQQALVREIIALRQQLLGVAEDD
                     AASLPDADAAEDTP"
BASE COUNT         2640 a         3085 c         3079 g         2521 t
ORIGIN      
        1 tttcgcaggt tcacacaatc tatttaacgc ttgcagaata cctctcttta cgatcgataa
       61 aaagtccttt tcttatttat tttgaagcgt agaatgcggg gtgatgaagg agtggtgatt
      121 ttattttcac cgccattagg gcggcattgc cgatattttt ttgcggtaca aatatgccgt
      181 gttcatggcc tgtacgagat aacatgacgg atcactttta tgagcaaaaa atttgaaggc
      241 agcgtcgcgc ctcgcgagcg tataaatata tcctatgtcc cgaagacgga cggccagacg
      301 gctgaagtgg agcttccgct taacatgctc gttgtcggcg ataccggaaa tacgcaggag
      361 acatccccac tggatgaacg tcaggcagtt tccgttaaca agcacaattt cggtgcggtg
      421 atggctgaag cagccattgg cctcaatttt accgtccccg ccacgctgaa gggaagcaca
      481 acggacgatg aactgaatgt ggcgctgaat atcaagtcac tggatgattt ttcaccggac
      541 agcgtggccc ggcaggttcc ggaagttaat aaattgctgg aattgcgtga agcactgact
      601 gcgctgaagg ggccgatggg caaccttccg gcattccgta cccagctcca ggcattgctg
      661 gaaaatgagg agtcccgcga gcagttgctc aaagaaatcg gccaagtgag tgggaaataa
      721 ccaccactat ttcattaaac agggttatcc agacatggcg gcacacaaaa atatctccgg
      781 gaaacacccc ctctctgacg aagacatttt gcaggcggtt taccgggtat gtttcagccc
      841 gtccggtgaa atcaaccggg atggcgtgga tgcgctgatt acaacactgg atcgccgtat
      901 cagtgatcta ttggatgaga ttatccatct ttgtgagttt cagcagagcg cgccgtactg
      961 gcggcgcgta cttcactgac ggcgaaaacc cggtttcaga aacacagcta aaacagaagg
     1021 attttcagta tgtctttaca ggaagaggaa ctcgtttcca gtcatgccgg gcagactgat
     1081 caggcatcgt cgctgcttga tcaaatcatg gctcagaccc gtattcagcc cggttcagaa
     1141 ggttatgacg ttgcccgcca gggtgtgaca gcctttattg ccagcatcct gcaatccact
     1201 gcatctgccg aaccggtcaa taaactggcc gtggacagca tgattgcaga tattgatgaa
     1261 cgcatcagcc gccagatgga tgtcatcatc catgcgcctg cctttcagca ggtcgagtcg
     1321 ttctggcgct cactgaaaac catggtggat cgcgttgatt tccgtgaaaa catcaaggtc
     1381 aacgtgcttc atgtcaccaa gcaggaactt ctggaagact ttgagtttgc gccagaaatt
     1441 attcagtccg gattttataa gcacgtttat tcgtccggtt tcggacagtt tggcggtgaa
     1501 cccatcgctg ccgtgctggg cgcttatgag ttcaaaaata ccgcgcctga catgaaactg
     1561 ctgcaatacg tcagtgccgt gggcgcaatg gcgcacgcac cgttcctgtc ctccgtttcc
     1621 ccggagttta tggggctgaa ctcgtggacc gaactgccta atatcaaaga tctttatgcc
     1681 atctttgaag gtccggccta caccaaatgg cgcgccctgc gtgactcgga agattcgcgc
     1741 tatttagggc ttacggcacc ccgcttcctg cttcgccagc cctattcacc aacagataac
     1801 cctgttaaga actttaacta ttacgaagat gtcagccaga accacgaaga ttatctgtgg
     1861 ggtaatacgg cctggatgct ggcatgcaat atcgcagaca gttttgccaa ataccgctgg
     1921 tgtccaaaca ttatcggtcc gcaaagcggc ggcgcagtga aagatctgcc ggtgcatctg
     1981 ttcgaaacga tggggcagat tcaggccaaa atcccaaccg aggtgctggt caccgatcgc
     2041 cgcgaatttg aactggctga agagggtttt atcaccctta ccatgcgtaa agactctgat
     2101 aatgcagcct ttttctctgc aaactcggta caaaaaccga agcactttcc gggaaaagac
     2161 gcagaaacca actataagct gggtacgcag cttccttatc tcttcatcat caatcggtta
     2221 gcgcactaca tcaaggtgtt gcagcgtgaa caactggggt catggaaaga aaggagcgac
     2281 ttagaaagag agcttaatac ctggatccgt cagtatgttg ccgaccagga aaacccgcct
     2341 gcagacgtgc gcagccgcaa acctctgcgc gctgccaaag ttgaggttat ggatgtagaa
     2401 ggcgaacccg gctggtacca ggttgcgcta agcgtgaggc ctcattttaa atttatgggg
     2461 gcaaattttg agctttccct ggttggccgg ttagacaggg agtaacacac catgataccg
     2521 gatcgggaaa gaaccacagg ttctcttttt gagcgtatgg aagcctcttc agccagaaac
     2581 cggcagggag gcagtattca ttcgctcagg caatccattc gccagaacct acgaaacatt
     2641 cttaataccc ggtccggtag ttgccggggt gcgcctgagc tgggcattga tgaaccggaa
     2701 ggggctgaaa acttccgcga atcaatgagt cgggccatcg cgcaatgtat agaacgctat
     2761 gaaccccgga tctcacgcgc tgaagtgcag gcagtggttt catccgcctc cagcccgctg
     2821 gatatgacgt ttcatattac agcctgggtg acatttaacg aaacgcatga ggtgcttgaa
     2881 tttgatatgg caccgaatgg cagccagcat taccgggtgg actgacaaat ggaatttgaa
     2941 gaacgctatt tccgggaaga actcgattac ctgcgccagc ttagcaagct gctggcaacg
     3001 gaaaaacccc atctggcccg cttcctggcc gaaaaagatg cagatccgga tattgaacgc
     3061 ctgctggaag gggtggcttt tcttaccggc aatctccgcc agaaaattga ggatgaattc
     3121 ccagaactga cgcacgggct tattaagatg ctatggccta attacctgcg tccggttccg
     3181 gcaatgaccc ttattgaata tacgccggat atggataagt cttctgtgcc ggtgttaatc
     3241 ccccgtaatg agcagtttac aaccaacgcc ggggaaatta gagtcgatga agtgctgccc
     3301 tccgatgcta aaaaggagga gccgcctccc tgtaccttca ccctctgccg ggatatctgg
     3361 ctgctgcccg ttcgcctgga gcagattgaa aaccgcagta cgacccgtaa tggtgttatc
     3421 aacatcacct tttcggtcgc accgggaacg gacttccgca cgctggatct gaacaaactt
     3481 cgcttctggc tcggcaatga cgacaactat acccgtgacc agctttattt atggttctgt
     3541 gaatacttgc agggtgccga cctgactgtg ggtgaacagc atattcgcct gcctgagttt
     3601 atgctgaaag ctgtcggttt tgagccacag gatgccatgc tgccctggcc gaaaaacgtc
     3661 cacagcggct accggatcct tcaggagtat ttctgttacc cggatgcgtt tctctttttt
     3721 gatctttgtg gttgtccggc gttgcctgac ggattgcagg ctgaatcctt taccctgcaa
     3781 ctgcgttttt cgcgcccatt gcccgtggac atccggttgc gccgcgactc cctgcgcctg
     3841 tattgcgcac ctgccattaa tttatttatc caccatgcag aagccatcac gctggacaac
     3901 cggcgggcag actatccgct ggttcccagc cgccattatc cggaacatta cgatgtcttt
     3961 tccgttaacg gcgtgataag ccaggtgcag gatatgttca ggaaaaaaga tctggggcgt
     4021 cctgtttcga cgcaggccgc gcgccagtgg cctgcctttg aaagtttcag ccatcagatg
     4081 gaatacagcc ggaagcggga agtggtgtac tggcatcacc ggaccaaaac atccctgttc
     4141 catcgcggct ttgatcatac ccttgctttt atacatgctg atggcagtta tccgtcagac
     4201 gaatcgctgc tcagtaatga agtggtttcg gtatcgctga cctgtaccaa ccgtgagctt
     4261 ccgtcacaaa ttcgttccgg cgatatcacc ggcacaaccg gtaaaaatgc ggctgttgct
     4321 tcatttcgca acattacccg cccgacgcaa ccgctctggc cggtcattga tggcagcctg
     4381 cactggtccc tgctctccgc catgaacctg aattatctgt cattactgga tacggacgcg
     4441 ctgaagcagg tcatcgccaa ctttgatcgc cacgcaatcc atcatccgca gacggcgcgg
     4501 ctgtcacaac aaaagctgga tgccattgag cgtctggaga ctcgccctgt cgaccgcctg
     4561 tttacgggca ttcccgtccg ggggctggcc tccacgctgt atctgcaccc ggagccgttt
     4621 gtctgtgaag gggaaatgta tctgctcggt acggtgcttt cgcattttct gtcgctgtac
     4681 gccagcgtta actcattcca catgctgacc gttgtgaaca cagaaagcca ggagacatgg
     4741 aaatggacgg aaagaaccgg gcagcatcct cttatctgag tccgggaaat cccccggcag
     4801 ataaagagca gaatgatccg ctggcacagg tttttcataa cgcctgctct tacaattttt
     4861 ttgcgatggc ggagctgttg caccgcctgg cacagggtga aaaggggacg ccggaattat
     4921 ccctgcgcga cgatccggca caggaaaccc tgcgtttcag cgccgatgcc agccttgcgt
     4981 ttcccctgag tgatatcagt gcgctgaaac gggatacgtc aggggcattc aggatgacca
     5041 ctacctttat ggggttacag gggagccagt cgcctctccc cggttattac ctcgatcacc
     5101 tggcctggaa agcggtacat gaacaaagtc cggtaggtga ttttctggat atgttcagcc
     5161 accgcctgac ccagtttgtc tggcatatct ggcgtaagta ccgctatcac atcagttttc
     5221 gtaacggcgg tgtggatgcg ttctcgcagc gcatgtactc ccttgttggg ctgggccacc
     5281 gccagcttcg cgataagctg gcgattaatc acagcaaaat gctggcgtat tccgggatcc
     5341 tggcaaatcc gggacgatcc ccggaaataa tctgcggtct ggtgtcccac tgttttgatt
     5401 tgtcagacgt gacgctgcaa aactggcaac gtcgcaaggt ggatattgaa ccggatcagc
     5461 aaaatagcct ggggagttat tcacttaaaa atggcgaaaa actggcagga agatcggtac
     5521 tgggcaactt tgttctgggt acacgggtgc ccgatctcag cggaaaattc cagctcagta
     5581 ttaccagcct gacccgaaaa cagttcctct cttttctgcc gtccggcgaa aacttcctgc
     5641 cactgacgat gtttgtgtcc ttcatcctgc gcgatcagct tgcctgggac ttacatctgg
     5701 ggctggcgcc ggagcaggtg ggcgcaatgc gcctgggcga taacaaaagt gcgctgctgg
     5761 gctggaccag cttcctcggc acaccggaag aacgaccttc agtcaccatc agggtacggt
     5821 cgtaaattat gataatcacg gagcagaata tgccggaaga gaaactacag acgctttcat
     5881 tgcaggtcat taacggcagt gagctggaaa gcgggcgggc ggcgcgctgt ctgttcacac
     5941 agcagggaaa tgtcggccat ggccccgaat gccactggtc ggtacaggat cgtcagcaga
     6001 gcattccggc tcaggctttt accgttatcc tgcacgatgg cacattttgt ctgcgcccgc
     6061 agacggcaca actgtggctg aaccaggcaa aagtcacagc aacatcagac ctgatacagc
     6121 tgcgccaggg cgatgagatc cagatagggc gtctgatggt gagggttcat ctgaaccggg
     6181 gagatatccc ccattacgat gaggaaatgg ccactcccga aaccatcgtc accaatcgcg
     6241 atatgctcac ggataccctg ctgtcaacgg agggtgcgcc acactatccg ggaatgactc
     6301 accggcatca gcttgcagac accgtggtaa acggtttttc taccgatcca ctccaggcac
     6361 ttcagtccga aagcctgatt accacgggcg atccgctttc aggcattgcg gctgtccggc
     6421 catcggcacc gctgtccgat ccggcaagta atggtgggat caatactccg tttatggatc
     6481 taccgcccat ttatgccagc cccggcgatc gtaatgatga cttccctgtg gcagaaatgg
     6541 cgcaacgcca ccttgcggtc acccccttac tgcgcggtct cggcggctcg cttaccgtga
     6601 gcaattctga cgatgcggat gattttctgg aagaggccgg acgcacgtta caggccgcaa
     6661 taaaaggtct gctcgatttg cagcaacagc gtaacagcct ctcagacaaa catttgcgcc
     6721 cgctggaaga taacccgctg cgcctgaaca tggattacgc caccgcgctc gacgtgatgt
     6781 ttgccgaagg taaaagtccg gtacatctgg cggctcccgc cgctgtcagt gaaagcctgc
     6841 gtaatgcccg ccaccacgaa gaagctaacc gggcagcgat tgtggagtca cttcgtgtcc
     6901 tgcttgacgc tttctcacca caaaatctgc tgcgccgctt tgtgcagtac cgccgcagcc
     6961 atgaactgcg ccagccgctg gatgatgccg gagcatggca aatgtacagc cattattacg
     7021 aagaactggc ctccgatcgc cagcaggggt ttgagatgct gtttaacgag gtctacgccc
     7081 agatctatga ccgggtgctt cgtgaaaaac agcgggagcc ggaagcatga acaggaaggc
     7141 gtttctggcc tgcgtgctga tgtgcgtcat attaaccggc tgcgaaacgg cgaaaaaaat
     7201 cagcgaggtg atcaaaaacc cggatatcca ggtcgggagc ctgaaggagc aaccttcgga
     7261 aataaccgtc accctgctga ccgaaccgga caccaatacc aatgcggagg gtgaatcagc
     7321 cgccgtggat gtgcaactgg tgtacctgac tgacgactca aaattgctgg cagccgacta
     7381 tgaccagata gccagtacgc cgctaccgga tgtgttgggt aaaaactata tcgaccacca
     7441 ggatttcaac ctgttgccgg acaccatcaa aaccctgccg ccggtaaagc tggatgagaa
     7501 aacgcagttt attggtgtcg tggcctattt ctccgatgac caggccactg aatggaaaca
     7561 aatcgagacg gtggaaggaa ccggccacca ttaccgcctg ctggtgcatg tacgtcaaag
     7621 cagcatcgaa atgaaaaaag aggatgaata attatggcaa ccacccgcaa caaggtgatg
     7681 tggcaggaag ggatgctgat gcgcccacac catttccagc aacagcagcg ttacaacgac
     7741 tacctggata accagcgttt ccgggccatg aatgatttat cctggggatt taccgaactc
     7801 accctcaaca atgaactgct ggcgcagggt aagatcatga ttgacagcgc gtcaggcaca
     7861 ctgcccgacg gcaccgtctt ttctatcccc gaccaggacg cactgcccga tccgctgcac
     7921 ccgcaacatt ttccggacga aagaagccgc aatatttacc tcgctctgcc ggtcgccagt
     7981 gatgtgagaa atgaaatcag cgacgggcgg cgaatcgggc gttaccggct gaattatgcc
     8041 gatgtccggg atctgcattc agaagaaggt gacgcgcgaa cactgacgct gggtcaactg
     8101 acgccgcgca ttatgagcgg tgcagaagat atgagcgcct atattacgct cccgctttgc
     8161 cgtatcagcg atcgccatgc cgacggctcc ctgacgctgg atgacgattt tatcccctcc
     8221 tgccagaata ttcaggtcag taagaaactg cgtgtttatc tcaaagaggt acagggggcc
     8281 attggcggac gggcaagcga tctggcaaac cgcattggct ctccggcgca gagcggcatc
     8341 gcggatgtgg cggaatttat gatgttgcag ttacttaacc gtaaccagac ccggtttacc
     8401 catcgcgctc gtcgatccca gctccacccg gaagatttct accttgatct tgccgggttg
     8461 ctgggtgaac tgatgacctt taccgagccg tcgcgcctgc cctgcccgct tgatgtgtat
     8521 gatcatcatg acctgaccaa aacatttaaa acgctgttac cggaagtcaa acgggcgctg
     8581 cataccgtac tgtcgccaag agccgttaat ctgccgctgc atctgcgtga cggtatctgg
     8641 caggccgata tccatgacac agaactgctg caatctgcca cctttgtgct ggccgtggcg
     8701 gcaaacatgc cggtcgatca gatccagcgc cagtttatcc agcagtcgaa aatttcctcg
     8761 ccggaaaaaa tccgcaatat ggtcagtgtg cagatacccg gcattccatt gcgtgccctg
     8821 atggtggctc cccgccagct tccttaccat tccgggttca gctatttcga actcgacaag
     8881 agcggccagg cctggacaga aatggctgcc gccggggccg ttgcactgca tgtatccggc
     8941 agtttcccgg atctgaacat gcaactgtgg gcgataagag ggtaagacga tggatgaggg
     9001 gtcactgtct ctgccaccat ttaccggaca ccacgaaaaa agccagcgcg actatcatct
     9061 cgcgcttcgt ggcaacagcc tgaatccgat gatcgacgca gccacgccgc tcctcggtat
     9121 ggtgatgcgc ctttcaacca tgaacagtca gacgatgccg gagcatcttt ttgctcaggt
     9181 ggtgacagat gttcaggccg tggagcaact gcttcaggag cagggctacg aaccgggcgt
     9241 gattatctcg tttcgctaca tcctctgcac gtttattgat gaagcggcgc tcggtaacgg
     9301 ctggtcgaac aagaacgagt ggatcaagca gtcattgctg gtccatttcc acaacgaagc
     9361 ctggggtgga gagaaagttt tcatcctgct ggagcgcctg atccgtgaac ccaaacgtta
     9421 ccaggatctg ctggaatttc tctggatctg tttttctctg ggatttcgcg ggcgctacaa
     9481 agtggcagca caggatcagg gggaatttga gcagatctac cgccgtctgt accacgttct
     9541 gcataaactg cgcggcgatg cccctttccc gctgttacac caggacaaaa aaacccaggg
     9601 cgggcgttat cagctcatca gtcgcctgac tgttaaacat attttttgtg gtggcgttgt
     9661 cgtgctggcg ttgttttacc tgttctatct gttgcggctg gacagccaga cgcaggacat
     9721 cctgcaccag ctaaacaaat tacttcgata aggacgttta tgatccagat tgatcttccc
     9781 acgctggtaa aacggctgaa cctgttctcc cgccaggcgc tggagatggc cgcctctgaa
     9841 tgtatgagtc agcaggcagc ggaaattacc gtcagccatg tgcttattca gatgctcgcc
     9901 atgccacgca gcgacctgcg ggttattacc cgccagggcg atattggcat ggaagagttg
     9961 cgccaggcgc tgacggtgga gaactacaca actgcccgtt ctgcggacag ctacccggca
    10021 ttttccccga tgctggttga gtggctgaaa gagggctggc tgctggcgtc ggctgagatg
    10081 cagcacagcg aattgcgtgg cggcgtgttg ctgctggccc tgctgcattc gccgctgcgt
    10141 tatataccgc ctgctgccgc ccggttgttg accggcatta accgtgaccg tctgcaacag
    10201 gactttgtac agtggacaca ggagtcggcg gaatcagtcg tgccggatgc agacggtaaa
    10261 gtcgcaggca cactgacgga cgctgctgac accctgcttg cccgctacgc caaaaacatg
    10321 accgcagacg cccgtaacgg caggcttgac ccggtactgt gccgcgacca cgaaatcgac
    10381 ctgatgatcg acattctctg ccgccgccgc aagaacaacc cggtggtggt gggcgaagca
    10441 ggcgtgggta aaagtgcctt aatcgaaggg ctggcgctgc gcatcgtggc aggccaggtg
    10501 ccggacaaac tgaaaaacac cgatatcatg acccttgacc taggcgcatt gcaggccggg
    10561 gcgtcggtga agggcgaatt cgaaaaacgc ttcaaggggc tgatggcgga ggtcatttcc
    10621 tctccggtgc cggtcattct gtttattgac gaagcacata ccctgattgg cgcgggcaac
    10681 cagcagggcg ggctggatat ctccaacctg ctcaaaccgg cgctggcgcg cggcgaactg
    10741 aaaaccatcg ccgccaccac ctggagcgaa tacaaaaaat acttcgagaa agatgccgcc
    10801 ctgtcgcgcc gcttccagct ggtgaaggtc agcgaaccca acgccgccga agccaccatt
    10861 attctgcgtg gtctgtcggc ggtctatgaa cagtctcacg gcgtgctgat tgatgatgac
    10921 gccttgcagg ccgcagcaac gctgagcgaa cgttatctct ccgggcgtca gttgccggac
    10981 aaagccattg atgtgctgga taccgcctgc gcccgcgtcg ccattaacct ttcctcgccg
    11041 ccgaagcaaa tctcggcgct gaccactctg agccaccagc aggaggcgga aattcgccag
    11101 cttgagcgcg agcttcgcat cgggctgcgt accaacacct cacggatgac cgaggtgctg
    11161 gtgcagtacg atgaaacgct gacggcgctg gatgaactgg aagcggcctg gcatcagcag
    11221 caggcgctgg tccgggagat tatcgcgctg cgccagcagt tgctgggcgt ggcagaagac
    11281 gatgcggcgt cgttgccgga cgcagatgcc gcggaggata cgccg
//
LOCUS       DACBVI010000141        11297 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.142, whole genome shotgun sequence.
ACCESSION   DACBVI010000141 DACBVI010000000
VERSION     DACBVI010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.142"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            204..308
                     /locus_tag="IU325_18240"
     CDS             204..308
                     /locus_tag="IU325_18240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HAP5810944.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            619..2817
                     /gene="speF"
                     /locus_tag="IU325_18245"
     CDS             619..2817
                     /gene="speF"
                     /locus_tag="IU325_18245"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HAP5810945.1"
                     /translation="MSELKIAVSRSCPDCFSTHRTCVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIIEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENGPGILVTQSVHKQQAGFSQTSQIHKKDNHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2814..4133
                     /gene="potE"
                     /locus_tag="IU325_18250"
     CDS             2814..4133
                     /gene="potE"
                     /locus_tag="IU325_18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HAP5810946.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCVIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4190..5830)
                     /locus_tag="IU325_18255"
     CDS             complement(4190..5830)
                     /locus_tag="IU325_18255"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HAP5810947.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASISGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5856..6401)
                     /gene="seqA"
                     /locus_tag="IU325_18260"
     CDS             complement(5856..6401)
                     /gene="seqA"
                     /locus_tag="IU325_18260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HAP5810948.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6586..7350
                     /locus_tag="IU325_18265"
     CDS             6586..7350
                     /locus_tag="IU325_18265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP5810949.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWEQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7421..7783
                     /locus_tag="IU325_18270"
     CDS             7421..7783
                     /locus_tag="IU325_18270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HAP5810950.1"
                     /translation="MYYGALSIRAEAWLIVSPEVTKIMAKEQTDRTTLDLFAHERRPG
                     RPKTNPLSRDEQLRINKRNQLKRDKVRGLKRVELKLNAEAVEALNELAESRNMSRSEL
                     IEEMLMQQLAALRSQGIV"
     gene            7923..8453
                     /gene="fldA"
                     /locus_tag="IU325_18275"
     CDS             7923..8453
                     /gene="fldA"
                     /locus_tag="IU325_18275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HAP5810951.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8663..8749
                     /locus_tag="IU325_18280"
     CDS             8663..8749
                     /locus_tag="IU325_18280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HAP5810952.1"
                     /translation="MIRIISRANSVTSRNEVNRLVTGQIPHD"
     gene            8742..9188
                     /gene="fur"
                     /locus_tag="IU325_18285"
     CDS             8742..9188
                     /gene="fur"
                     /locus_tag="IU325_18285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HAP5810953.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9273..9599)
                     /locus_tag="IU325_18290"
     CDS             complement(9273..9599)
                     /locus_tag="IU325_18290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810954.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9649..11055)
                     /gene="chiP"
                     /locus_tag="IU325_18295"
     CDS             complement(9649..11055)
                     /gene="chiP"
                     /locus_tag="IU325_18295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HAP5810955.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2757 a         2786 c         2805 g         2949 t
ORIGIN      
        1 gcaggtttaa tttcttaacg caaatataaa acacgaaatt acaacattta agtaacaaaa
       61 cagcatctaa tatggcaata ttcataatta atgatttatt atctggggta tgatcgtttt
      121 ttgttgatct tcttcacaga ttatagccat ttcatggata gaataactct accttcaact
      181 gacacagcaa gaggtaaagg taaatggaaa ataataaccg cttaatgcct catataaggc
      241 ggacaaccca tatcatgaag tttgcccatc gtaatagttt tgactttcat ttctttaatg
      301 cccgctagtc ttctgactaa agggcacccc aacgtacagg tctccctgac tttaagcatt
      361 acaggttaat acctgtatcc ctcggtgctc atatactgct aaccctttta aactctaaat
      421 aattcgagtc gcagcacttg caacttgagg tatgacgagt atagccagtt accgggctgg
      481 tctgggttat tgcatctaca aaaagcaaac tactgattta tttatcagcg gtggggtttt
      541 gctttttttc cggcgtgatc gatttctcct ttgagaaatt gaggacctgc tattacctga
      601 aataaagaga tgaacaaaat gtcagaatta aaaattgccg ttagtcgttc ttgcccggat
      661 tgtttttcaa ctcatcgcac atgcgtgaat attgacgaaa gtaattatat tgacgttgcc
      721 gccattattt tatcggtcaa tgatgtggaa cgtggaaaac tcgatgaaat agacgctact
      781 ggctatggca ttcctgtttt tattgcaacg gaaaatgaag aacgtgtacc cgcagaatat
      841 ctgccacgta tttctggtgt cttcgaacat tgcgaatcac gtaaagagtt ttatggacgt
      901 cagttagaaa ccgctgcaag ccattatgaa acgcaactgc gcccgccctt cttccgcgca
      961 ctggtcgatt atgttaatca gggcaatagt gcttttgact gccctggtca tcaggggggt
     1021 gagtttttcc gtcgccatcc ggcaggcaat cagttcgtgg aatactttgg tgaggcgctg
     1081 ttccgtgctg acttgtgcaa cgccgacgta gcgatgggcg atctgctgat tcacgaaggc
     1141 gcgccatgca ttgcacagca acatgcggct aaagtcttta atgccgataa aacctatttc
     1201 gttttaaacg gtacttcatc ttctaataaa gtggtcttaa acgcattgct gacgccgggc
     1261 gatctggtgt tgtttgaccg caataaccat aaatctaacc accacggtgc gttgctacag
     1321 gctggcgcga caccggttta tctggaaacc gcacgtaacc cgtatggttt tatcggtggt
     1381 attgacgctc actgttttga agaaagttac ctgcgtgagc tgattataga agttgcaccg
     1441 cagcgggcaa aagaggctcg tcctttccgc ctcgccgtta ttcagctagg cacttacgac
     1501 ggtacgattt ataacgcccg tcaggtggtg gataaaattg gtcatctgtg tgactacatc
     1561 ctctttgact ccgcatgggt cggctatgag cagtttatcc cgatgatggc ggactgttca
     1621 ccactgttgc tggatcttaa tgagaacggt ccgggtattc tggttacgca atccgtacat
     1681 aaacagcagg ctggtttttc tcaaacttca caaatccata aaaaagacaa ccacatcaaa
     1741 gggcaacagc gttatgtacc gcacaaacgc atgaacaacg cctttatgat gcacgcctcc
     1801 accagcccgt tctatccgct gtttgccgca ctggatatca acgccaaaat gcatgaaggt
     1861 gtcagcggtc gtaatatgtg gatggattgt gtggtaaatg gcattaatgc ccgcaaactg
     1921 atcctcgata actgtcagca tattcgtccg ttcgtacctg aactggtgga tggtaaaccc
     1981 tggcagtcgt atgaaacagc gcaaattgcg gttgatctgc gcttcttcca gtttgtacca
     2041 ggggaacact ggcattcttt tgaaggctat gcagagaatc aatactttgt tgatccgtgc
     2101 aaactgttgc tgacaacccc aggtattgat gcacgtaacg gcgaatatga agcgttcggt
     2161 gtacccgcga cgattcttgc taatttcctg cgcgaaaatg gcgtagtgcc ggaaaaatgc
     2221 gatcttaact ccatcctctt cctgctgact ccagcagaag atatggccaa acttcagcaa
     2281 cttgttgccc tgctggtacg cttcgaaaaa ctgctggaat ctgatgcgcc gctggcggaa
     2341 gtgctacctt ccatctacaa acagcatgaa gagcgctacg ccggttatac gcttcgtcag
     2401 ttgtgccagg aaatgcatga tttgtatgcc cgccacaacg tgaaacaact gcaaaaagag
     2461 atgttccgta aggaacactt cccacgcgtc agcatgaatc cgcaagaagc taactacgcc
     2521 tatttacgcg gtgaagtgga gctggttcgt ctgccggatg cagaaggccg tatcgctgca
     2581 gaaggtgcgc ttccttatcc tccgggtgtg ctgtgtgttg ttccgggtga aatctggggt
     2641 ggtgctgttt tgcgttactt cagcgctctg gaagaaggga tcaacctgct gccaggtttt
     2701 gcaccggagc tgcagggtgt ctatatcgaa gaacatgatg gtcgtaagca agtttggtgc
     2761 tatgtcatca agcctcgtga tgcgcaaagc accctgttga aaggggaaaa attatgagtc
     2821 aggctaaatc gaacaaaatg ggcgtcgttc agttaaccat actgacgatg gtcaacatga
     2881 tgggctccgg tatcatcatg ctgccgacaa agcttgccga agttgggaca atttcaatta
     2941 tctcctggct ggtgacagcc gtaggctcaa tggcactggc atgggcattc gcaaaatgcg
     3001 gtatgttcag ccgtaaatca ggtggtatgg gcggttatgc cgagtatgct ttcggtaaat
     3061 ccggtaactt tatggcgaac tatacctacg gcgtctcact gctgattgct aacgtcgcga
     3121 ttgctatttc ggcggttggt tacggcaccg aactgctcgg cgcgagtttg tcgccagtgc
     3181 agattggtct tgcgaccatc ggggtgctgt ggatttgtac cgtggctaac tttggtggtg
     3241 cgcgcattac cgggcaaatc agtagcatta ccgtgtgggg ggtcattatt ccggtggtag
     3301 gcctgtgcgt tatcggctgg ttctggttta gcccgacgct ttacgttgat tcctggaatc
     3361 cgcatcatgc accgttcttc agtgcggtag gttcttccat cgctatgacg ctgtgggctt
     3421 ttcttggtct ggagtctgcg tgtgcgaata ctgatgtagt ggaaaacccg gaacgtaatg
     3481 tgccaatcgc ggtactcggc ggtacgttag gtgcggcggt gatttatatc gtctccacca
     3541 acgtgattgc cgggattgtg cctaatatgg agctggcaaa ttcaacggca ccatttggcc
     3601 tggccttcgc acagatgttc acgccagaag tgggtaaagt cattatggcg ctgatggtga
     3661 tgtcctgctg cggttcgctg cttggctggc agttcaccat tgcccaggtg tttaaatctt
     3721 catctgatga aggctacttc cctaaaattt tctcccgtgt aaccaaagtg gatgcaccgg
     3781 tgcagggaat gttgaccatt gtgattatcc agagcgggtt ggcgctgatg acgattagcc
     3841 cgtcgctaaa cagtcagttc aacgtgctgg ttaacctggc cgtggtgacc aatatcattc
     3901 cgtatattct gtcaatggcg gcattagtca ttattcagaa ggtcgctaat gtgcccccct
     3961 caaaagcgaa agttgcaaac tttgttgctt ttgttggcgc gatgtacagc ttttatgcgc
     4021 tgtactcatc cggggaagaa gccatgctat acggttccat cgtgaccttc ctcggttgga
     4081 cactgtatgg tctggtctca ccacgctttg aactgaaaaa taaacacggt taattaagca
     4141 aagcaaaaaa gggcgatctt ccgaccgccc tttttcttat ttaatgtgtt tacgcgtttt
     4201 tcagaacttc gctaacaatc tcaaccgctt ctttctcgat ctgcttgcga tgctcttcac
     4261 cgaggaagct ttcgcaataa atcttgtacg cgtcttccgt gcctgacgga cgcgcggcga
     4321 accagccgtt gtcagtcatc actttcagac cgctgataga agcaccgttg cccggtgccg
     4381 ccgtcaggcg cgcggtgatc gggtcgcctg ccagggtgct ggcgctcacc atttccggag
     4441 acagcttaga cagcgccgct ttttgtgcgg aagtcgcggc tgcctgcaaa cggttgtagc
     4501 tcggcgcacc aaagcgtttt gccagttcgt tgtagtgttc ctgcggattc ttaccggtga
     4561 cagcggtgat ttccgccgcc agcagacaca taatgatgcc gtctttatcg gtggaccacg
     4621 gcgtgccgtc gaaacgcagg aaggaagcgc ctgcactctc ttcgccgcca aagccgaagc
     4681 tgccatcaaa caaaccatca acaaaccatt tgaaacctac cgggacttct accagcttac
     4741 ggcccaaatc attgaccaca cggtcgatca tcgcagatga aaccagcgtt ttaccgacag
     4801 caacatcttt gccccactgc ggacggtgct ggaacaggta gttgatcgcc accgccaggt
     4861 agtggttcgg attcatcaaa cctgccggag tgacgatacc gtgacggtca taatccgggt
     4921 cgttagcaaa cgccagatcg aacttatcac gcagtgccag cagacccgcc atcgcacact
     4981 cggaggagca gtccatacgg atcgcgccgt ctttatcgag gtgcataaag cggaaggttt
     5041 gatcgacctg atcgttaacg atggtcaggt tgagattgta atactcgcca atgcgcttcc
     5101 agtattcgat accggaaccg cccagcggat caacgccaag cgtcaggccc gctttctgaa
     5161 tcgccgccat atcaacgata tcggccagcc cttccacgaa cggctgcacc agatcctgct
     5221 ctttcacatg accggatgcc atcgcttcgt cgagggagat acgcttcaca cctttcaggc
     5281 catcggccat cagtgcgttg gccctgtctt ccaccacttt ggtgacgttg gtatcagccg
     5341 ggccaccatt tggcgggttg tacttgatac caccatcttc cggcgggtta tgggacggtg
     5401 taatcacgat accgtctgcc agcgggccac cttttttatt gtgaaccagg atggcattgg
     5461 aaacggcagg cgttggagtg aagccattgt tttcctgcac aataacatca acgccgttcg
     5521 ctgccagcac ttccagaacg gaaatgaacg caggctcgga cagggcgtga gtatctttac
     5581 ccacatagca agggccagtg atgccgtttt tcgcacgttc ttcagcaatt gcctgagcga
     5641 tcgccagaat gtgcggctcg ttaaagctgt ggcgcgctgc actgccacgg tgaccggaag
     5701 taccgaattt caccgcgtgc tccgcattcc ctgcttctgg tttcagtaca taatattgcg
     5761 ccgtcagttg ggcgacgtta atcaaatcac tctgttgtgc aggttggcct gcacgattgt
     5821 ggattgccat tgctttgtcc tttgtctgca acgttttaga tagttccgca aaccttctca
     5881 atcaattccg ccgggaattg catcgactgc atgatgtgtt cgatcatgct gcatttacgg
     5941 ccggtgttgg tgttggtgat cacccaatac ggcgtgccag gcacatgttt cggcttggtc
     6001 tgattaccat ttttcagcag cgtttgttca tctgccgcaa agtaaacgcg tgtacgaccg
     6061 tgcaacgatt ccgttgcctc ggcaaacgcc tgggcgtcaa gagaatatag tgtagacaac
     6121 agcagcataa agcgattgac cgctcgcttt tgctctgcgt attcatccga aagcagaagt
     6181 tcacgcattg cgcgaacctt gtctttaatc gttttgaccg gcttcgcttc gacgatagca
     6241 ggtgacgcaa cgcgaacctc tttcgtcacc ggagcagcag gctgtgatgc ggcggaaaat
     6301 ttcaacatac gccgtaaaat gtcggatgcg ctctcgccga tatgcttagt gtggctggca
     6361 atatagctgt agagttcatc atcaacttca atcgttttca tcttaatcca gtgcagtgtc
     6421 ttagctgaat acaagtcgcc aggagtatat gaataaatcc cggcagcgga tagcgtcaag
     6481 cctggcttgc agaaaaactc ggaataaacc ttatttgttg cagccctgtg gcacaatggt
     6541 caacataata ccctaacccg acaacgcgaa aaaaagaact ttgccatgaa attgaatatc
     6601 cgcgcgcaaa ctgcacaaaa ccagcacaat aattctccca tcgttcttgt ccatggtctg
     6661 tttggcagcc tcgacaacct tggcgtacta gctcgcgatc tggtaaacga tcacaatatc
     6721 atccaggttg atatgcgtaa ccacggtctt tcaccgagag atccggtaat gaattacccg
     6781 gcgatggcgc aggatcttgt tgataccctg gatgcactgc agatcgataa agcaacattt
     6841 atcggtcact ccatgggcgg taaagcggta atggcactta ctgcactggc ccccgatcgc
     6901 atcgataaac tggtggcgat tgacatcgcg ccggtcgact atcacgtacg ccgtcatgat
     6961 gagattttcg cggctatcaa cgcagtcagt gaatcggacg cacaaactcg ccagcaagca
     7021 gcagcaataa tgcgccagca tcttaatgaa gaaggggtga ttcagtttct gttgaaatct
     7081 tttgttgacg gggagtggcg ctttaacgtg ccggtattgt gggaacagta tccgcatatt
     7141 gtgggttggg agaaaatccc ggcatgggat caccctgccc tgtttatccc aggcggcaat
     7201 tctccgtatg ttagcgagca gtatcgtgat gatttactgg cgcaatttcc acaggcgcgg
     7261 gcgcatgtga ttgctggcgc gggtcactgg gtccatgctg aaaaaccgga tgcggtatta
     7321 cgcgctatcc gtcgctatct caatgattaa cgcaactgat taaaaaccca gcgtccgcgt
     7381 cgctttccgc gcgtgggcgt tggcgtgact catcgcgctg atgtattatg gcgcgctatc
     7441 catccgggca gaggcctggc tgattgtttc ccccgaagtc accaagatca tggccaaaga
     7501 acaaacggac cgtacgacat tagatctgtt cgcgcacgag cgtcgaccgg gacgaccgaa
     7561 aactaatccg ctttcgcgcg atgaacagct gcgtattaat aaacgcaacc agctaaaacg
     7621 cgacaaagta cgtggcctta agcgtgtcga actgaagctg aacgcggaag ctgtcgaggc
     7681 gctgaacgag ctggcagaat cgcgcaatat gagccgtagc gaactgatcg aagagatgtt
     7741 aatgcagcaa ctggcggcgc tgcgtagtca gggcatcgtt taaatttcca ctttcatgta
     7801 gcacagtgtg cagtcctgct cgtttgctga ttccgcaaga ctgcctgttc tgctatgatt
     7861 gcctttatcc gtgggcaatt ttccaccccc atttcaataa gtttcaagag gttatttcac
     7921 tcatggctat cactggcatc tttttcggca gcgacaccgg taataccgaa aatatcgcaa
     7981 aaatgattca aaaacagctt ggtaaagacg ttgccgatgt ccatgacatt gcaaaaagca
     8041 gcaaagaaga tctggaagca tatgacattc tgctgctggg catcccaaca tggtattacg
     8101 gcgaagcgca gtgtgactgg gatgacttct tcccgactct cgaagagatt gatttcaacg
     8161 gcaaactggt tgcgctgttt ggttgtggtg accaggaaga ttacgccgaa tatttctgcg
     8221 acgcattggg caccatccgc gacatcattg aaccgcgtgg cgcaaccatc gttggtcact
     8281 ggccaactgc gggctaccat ttcgaagcgt caaaaggtct ggcagatgac gaccactttg
     8341 tcggtctggc tatcgacgaa gaccgtcagc cggaactgac cgctgaacgt gtagaaaaat
     8401 gggttaaaca gatttctgaa gagttgcatc tcgacgaaat tctcaatgcc tgatgtgatg
     8461 cggcgtagac tcatgtctac gccgtattaa tagataatgc caatcaaaat aattgctaca
     8521 aatttgtaac ttttgctgtt gtacctgtac aatgtcccgg tgttcaagtg gccttgccgt
     8581 tgtaaatgta agcagtgcca cgtttttatt aacaatattt gccagggact tgtggttttc
     8641 atttaggcgt ggcaattcta taatgatacg cattatctca agagcaaatt ctgtcacttc
     8701 tcgtaatgaa gtgaaccgct tagtaacagg acagattccg catgactgat aacaataccg
     8761 ccctaaagaa agctggcctg aaagtaacgc ttcctcgttt aaaaatcctg gaagttcttc
     8821 aggagccgga caaccatcac gtcagtgcgg aagatttata caaacgtctg atcgatatgg
     8881 gtgaagaaat tggtctggct acggtatatc gcgtactgaa ccagtttgac gacgctggta
     8941 tcgtcacccg ccacaatttt gaaggcggta aatccgtatt tgaactgaca cagcaacatc
     9001 accacgatca cctgatctgc ctcgactgcg gcaaggtaat cgaatttagt gatgactcca
     9061 tcgaagcgcg tcagcgtgaa attgccgcaa aacatggcat tcgcctgact aaccacagtc
     9121 tctatcttta cggtcactgt gccgaaggcg attgccgcga agatgagcac gcgcacgaag
     9181 gcaaataagc cagcctgaat gagaaaaagc caacctgcgg gttggctttt ttatacacgg
     9241 gaaataagaa aagcgagagt tacagctctc acttatttgt tattactgcg aatttcctgc
     9301 caaactttat cacaatcggc tttcaccgcc tgatcatttc cggtttgcgt tgcacgcaga
     9361 cactgctgat aatccagtac gcgtacactt tcctggtcgg caaactgctg atgttgtttc
     9421 tctttcttca gcacgtttaa cacgctctgg caggcttcaa ttttttctgg cgaaccttgt
     9481 gcggtgttga tacaggcgct gtatgcctct ttcagacggc tgtcttcctg tggcgcggtt
     9541 gattgcgcac aagccaccag ccccgatgcc atcatggcga tgagaatcag ttttttcata
     9601 gcaaattcct caaacgtgcg gcaggcacaa aacctgccgc gtcgggcatc agaagatggt
     9661 gaatggtgcg attaccataa atttcacgtc acgctcatcc tggaaaatgt tgccgtaacc
     9721 accgccccag cttgggatgt cggagtggtt gtcgtattcg gtgaagtgca gtttgaacat
     9781 cgtgcctttg gcgcgaccgt cctgaatggt gtagaccgca tccaggctgt aggcagactc
     9841 ttcaatagtc cggtttttgt cgtagtacgc atccggattg ctctgccagg tagcgggttt
     9901 agcatcccat gcgtaaacgt aggaagcgcc gatggcgaag cctggaagat tccagttttt
     9961 caagtcatac atcgcaccga agaagaccgc tttttcgccg ttggcgttga agtcagaacg
    10021 gttatcccac cagatatcca ggcgaccgtt cgaggaagcg taggttggag tcatacgttg
    10081 cagaaagtat ccctgctgac cgtcggcttt aacccaggtg ccttccagac gcaaatccac
    10141 tacatcagcc gcccggtaac caaaggtcaa cgcttgcagc caggcagtgc cgtcgtaaag
    10201 gtcgttgacg ctgcgatcgt caactttatc gcgcgtaccg tagaactggt agctggtggt
    10261 taacgggcta ccggcgatat caaatttgta gctggctttg gcaaagtatt gatcgatata
    10321 cccttccgcc tgaccaaatg ctgcttccag tacgaagtta tttttgaagt cgtatttcgc
    10381 accgatggag tgcagataat caactttggt ggttttatcg ttctgataaa actcatccat
    10441 ttccagatgc cacggtgctt tgtattcgtt ggtccacatg taggagaaac tcaacgcacc
    10501 cgcatcgccg taatcaaaat tcgccccggc ttccgccccc tgataagtac ccggcataaa
    10561 gctccagtgc ggcgctaaca gcgtttgacc agttggctga atgtaacctg ccctcgccca
    10621 aaccggaccg tatttaaatt tggccgctgc tttatacagg cttataccgc ttttatcgcc
    10681 ggaccagtct tcgtcatagg ctttattact ttttgaaaac gcgatttcgt tcgggtgaga
    10741 gctgtcaccg ttttccgcca tttcaatcgc cgtaaacgcg gcaatatcaa ggccgaacat
    10801 atcagcagca taaccggact gaaaatcgag gttggcgttc caggtggagt gagaaaggtt
    10861 ggttttgtat ttgtcgccgt cggtaacatc tttacggtcg cgttcacgct gccagtaata
    10921 gataccgccg gttaaggttg aatcgtcgat gaatccttcg gcgcgagcct ccggcattgc
    10981 cataaagccc gacattgctg taacaccggc gatagccagc gccagcgtac tacgtttgcc
    11041 actaaacgta cgcatgggtt aatcctcttt gacgtataaa ttgctgcacc aaaggtgaac
    11101 agcgaaaaaa ataaaaatta aaaaagtagc gttgagaaaa gtctcgctga ctacaggaac
    11161 agactatttt tcgcgacgcg aattatcaat ctctttctgt aaccagaatg taagaacatg
    11221 agcaaccgca catattttga tgatttttgt tacaaggctt aagttgtagt tgaaaggtgt
    11281 attgaagaaa aaatgcg
//
LOCUS       DACBVI010000142        11263 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.143, whole genome shotgun sequence.
ACCESSION   DACBVI010000142 DACBVI010000000
VERSION     DACBVI010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.143"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..875
                     /locus_tag="IU325_18300"
     CDS             <1..875
                     /locus_tag="IU325_18300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810956.1"
                     /translation="VLSGIIGNVPKNNKPKTIIFDLRFLSAIKQKVFLGSEKTLFVKH
                     TAMMREACKELPQSVEYVPLACEAHSHRSVALAKTVAAPIMATGVGSALMYATSSGPY
                     YAQSLSGSPDIEMIKNRMAELFNQLDISARNKYRPAFDKWNELVDKLNHERNTVPFAC
                     LTTILATAINETPEGDTNVAVVMGCKSAKDRTISIVLGNSMLQTLLEKRLADGREIEK
                     LFDQQGYFSCDSLTAKELMMLKDLFDIRVLHLSNKFNVGLQGNINTDVLQDSFFKNVD
                     FIHESNSYTVGMGV"
     gene            complement(1043..1951)
                     /locus_tag="IU325_18305"
     CDS             complement(1043..1951)
                     /locus_tag="IU325_18305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HAP5810957.1"
                     /translation="MALEKGIAELVEEFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMILRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QYRVIAVQYRLAPEQTFPAAHDDAQRGAELVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            2180..2491
                     /locus_tag="IU325_18310"
     CDS             2180..2491
                     /locus_tag="IU325_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP5810958.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            2492..2782
                     /locus_tag="IU325_18315"
     CDS             2492..2782
                     /locus_tag="IU325_18315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP5810959.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRAS
                     GLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            2867..3078
                     /locus_tag="IU325_18320"
                     /pseudo
     CDS             2867..3078
                     /locus_tag="IU325_18320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197769.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3140..3418
                     /locus_tag="IU325_18325"
     CDS             3140..3418
                     /locus_tag="IU325_18325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810960.1"
                     /translation="MSTLNTRKDRDRNGKAKLHNHSGIPTLTGKWRHPSMDCWVNYST
                     PGDHEWKRLYTTLRRRADDRRQCHRVMQGDDPEGIVWFPDCYPQIYYY"
     gene            3837..4055
                     /locus_tag="IU325_18330"
     CDS             3837..4055
                     /locus_tag="IU325_18330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HAP5810961.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            4274..4516
                     /locus_tag="IU325_18335"
     CDS             4274..4516
                     /locus_tag="IU325_18335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5810962.1"
                     /translation="MNSLAGIDMGRILLNLSNELFKQLDDLEVQRNLPRAELLREAVD
                     QYLVNQSQTARASAFGIWQGSEEDGVEYQRKLREEW"
     gene            complement(4698..5627)
                     /gene="fdhE"
                     /locus_tag="IU325_18340"
     CDS             complement(4698..5627)
                     /gene="fdhE"
                     /locus_tag="IU325_18340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HAP5810963.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTTRIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(5624..6259)
                     /gene="fdoI"
                     /locus_tag="IU325_18345"
     CDS             complement(5624..6259)
                     /gene="fdoI"
                     /locus_tag="IU325_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAP5810964.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(6256..7158)
                     /gene="fdxH"
                     /locus_tag="IU325_18350"
     CDS             complement(6256..7158)
                     /gene="fdxH"
                     /locus_tag="IU325_18350"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAP5810965.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(7171..10221)
                     /gene="fdnG"
                     /locus_tag="IU325_18355"
     CDS             complement(7171..10221)
                     /gene="fdnG"
                     /locus_tag="IU325_18355"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(9634..9636),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAP5810966.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVISCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            10415..11248
                     /gene="fdhD"
                     /locus_tag="IU325_18360"
     CDS             10415..11248
                     /gene="fdhD"
                     /locus_tag="IU325_18360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HAP5810967.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2770 a         2750 c         2910 g         2833 t
ORIGIN      
        1 aggttcttag cgggataata ggaaatgtgc caaaaaataa taaaccaaaa acaatcattt
       61 ttgatttacg ttttcttagt gcaattaagc agaaagtttt tttgggcagt gaaaaaacgt
      121 tgtttgtaaa gcataccgca atgatgaggg aggcatgtaa ggaattaccg cagtcagtgg
      181 aatatgttcc tttagcctgt gaagcgcata gccatcgcag cgtcgccctt gccaaaaccg
      241 tagctgcgcc catcatggcg acaggcgtgg gatcggcttt aatgtatgca acatcatcag
      301 gaccgtatta tgcgcaaagt ctttctggtt cgccggatat cgagatgata aaaaatcgca
      361 tggctgaatt gtttaaccag ttggatattt ctgcgcgcaa caaatatcgc ccggcttttg
      421 ataaatggaa tgagctggta gataaattaa atcacgaaag aaatacagtt ccgtttgcgt
      481 gtttgaccac cattttagcg acggcgataa atgaaactcc tgagggggat acgaatgttg
      541 ccgttgtgat ggggtgtaaa tctgcgaaag atcgtacgat atccattgtc cttggcaact
      601 cgatgttgca aacactcctt gaaaaacgcc tcgccgatgg tagagaaata gaaaagcttt
      661 ttgatcaaca gggatatttt agttgcgatt cattaacagc caaagaatta atgatgttaa
      721 aagacctttt tgatattcga gttcttcatc tctctaacaa atttaatgtt gggttgcaag
      781 gtaatatcaa cacggatgtt ttacaagatt cgttttttaa aaatgtcgat ttcattcatg
      841 aatctaactc gtataccgta gggatgggag tctgaagagt aaaactcatt agaattacca
      901 gaattcggcc aatgcattgc aaaaatgaca ttcaaagaga gatgtgtgca ggcgcttagc
      961 cgcgaggggc atggcgaagc catacagaaa gcaccagcat tcagaactcc tgtgaaagcg
     1021 gcatagtgcc gctgctcagg agttaccttc ccgctctcca ggccacatcc cgcagcgccg
     1081 atcgcgcagc ctcactgata ccacctaact gaaaaaatcc gtggataacg ccaaggtagc
     1141 gttggcaggt gcattgcact ccttgccctg tcatgcggtg atacaacgct tcaccttcat
     1201 cgcaaagtgg gtcgtattca gcggtaatga tatgtacagg tggcaagcca ttgaaatcat
     1261 cgcgccaaag agggctggct tcaggatgcc tgcgttcaat gcctggcaga tacatctcaa
     1321 agccgctcag taaagtgtcg cgtgtgatga tgtaatcgtg accattgcgt atgtagcttt
     1381 cgaaatgcgc agtcgcatca agcatgggat aaattaagat gagttgtgcc ggttgccact
     1441 cgccagcacg ctttaatcgc aaagcagtta ccagcgccag atgtccgccc gcactgtcac
     1501 cggcaagagt tatccgttgt ttatcaacgc cgagtcgttc ggcgtgttgc cagactaatt
     1561 ctgcacctcg ttgcgcatca tcatgggcgg cggggaaagt ctgttccggt gccagccgat
     1621 actggacggc aataacccga tattgcccgt aataggctaa ctggcgcagc tggttgtcgt
     1681 gagttgcgaa cccgccgcta acaaaacagc caccgtagta ataaattatc gtcgggagta
     1741 atgtcggggc attcagcggt gacactattc gtaagatcat cccttcaagt tcgatagtct
     1801 gaatatctac ccgcgtttct gtctcacccg caaggacagc gctggcgata tagccctccc
     1861 tgcgctgcgc aatactttgt tgacgcgatg aggggcgacc cgcagcgata aactcttcga
     1921 ctaactctgc aattcctttt tccagtgcca tagtagaact cattttgctg tatgaatgta
     1981 cagttttata cacccgtttt tgctggatgg atacagggaa attcacgggc gtgaaaaatc
     2041 tggaaagcgc ctcgcaaatg taaagacaaa cccggcagag acgtggtttt gctggagcac
     2101 tatcagtaag ctagtggttt caagggtacg tcaatgacgt acaaaaagga ttgttactct
     2161 aagccaatgg cgtataatta tgctcttcat cgaaacggaa atttttactg aagatgttca
     2221 aaaactgctg accgacgatg aattcagtcg ttttcagttt ttcctggcct tgaatcctga
     2281 ctatggtgag gtaatacctg aaactggtgg gttaagaaag gttcgatggg tgtcaggagg
     2341 aaaggggaaa cgtgctggtg tcagggttat ttactttcat caggttaaac attatgagat
     2401 cagattactg cttatttatc gtaagggtat taaagatgat ctgtctcccc aggaaaaagc
     2461 aatgctccgg ttgttaaata cgaggtggta aatggataag gcactttttg agcggttgac
     2521 tcacagcatg gcccagatga atgaaattat tgaaggaacg cgacaccctt cacggacttt
     2581 tgaagttgat gctatgaaaa tcaaagaaat acgccgcgct tcagggttgt cacaatctaa
     2641 atttgccgaa cttatttccg taagcgtaga cacgttgcgt aactgggagc aggggcgacg
     2701 ttcaccgact gggcctgcaa aggctttgct gcgagcgatt gccaatgatc cgcaacatgt
     2761 gctgcaggca cttaaccgtt agaggcatgg ttatgccata cagaaagcac cagcattcag
     2821 aactcctatg aaagcggcat agtgccgcct gctcaggagt tttactatga cccgtaatcg
     2881 tgctgaacgt cgccatcata tggcgcgatt aaaaaaatcc gctgccgtta tcgcactgca
     2941 ggcgatggaa gcaaaaaagc atcaggtatt tgctttcgta ctccctgtat ttgttcttgt
     3001 tggatgtgtg ggcatcgacg ctatcataat ggtccccgcg tatcggaaat tcgtgcaaaa
     3061 gcacgctatg cgaattaagg gaaaatgatg aactatttta aggaagcaca tttttagaac
     3121 tcttaacagg agactaaaaa tgtccacgct taatacgcgt aaagataggg atcgtaatgg
     3181 taaagcgaag ctgcataatc attcagggat cccaacactt acaggtaaat ggcgtcaccc
     3241 ttcaatggat tgttgggtaa actacagtac accgggcgat catgaatgga agcggctcta
     3301 tactacgctt cgccgtcgcg cagatgatcg acgccaatgt catcgggtaa tgcaggggga
     3361 tgatccagaa ggaatcgtct ggttcccgga ctgttatcca caaatttact actactaaca
     3421 attaaagacc ggttggtgcg gcactgaccg gtcagttatg aatggtagat ttcaccttaa
     3481 accctggtgc ggtaaagctg ctgacttttt cgggtacacc atcctgatga agcaaatgaa
     3541 cacccaatca aaaaaatatt cttgaatata aaatatttat agcacccata attcatcctt
     3601 attatttgtt gtttcttttc tgtagatgtg tccccgctta attaaaagta ataaaccatc
     3661 ctgggttatt actaaaccat ttttgagtaa caattgaaat aactgcaact gttacattat
     3721 ctttggatgc cgtctcgcaa aaacaaaaca gcatgcaaaa atccgctttt tttaaatgat
     3781 ttttcggcgt aattttcgca catacattaa tgagtaatat atgtaaggta ttggtaatgg
     3841 cgatgaatac ggtttttctt catttatcag aagaggcaat taaacggctg aacaagctta
     3901 ggggctggcg taaggtttca cgatctgcaa ttttacgcga agcggtagag caatatctgg
     3961 aacgtcagca attcccggtg cgtaaagcaa agggaggcag gcaaaagggg gaggtagttg
     4021 gtgttgatga tcagtgtaag gagcataagg aatgacaaaa atttttttat tcttgaatat
     4081 aaaaaccaga tacccttatt gtggtattaa tacaaggttg ttttacttga acttataata
     4141 actgcaactg ttacatcata tctggaaaac gcctcgcaaa aataaaaaat gatgcgtaaa
     4201 tgagctttct ttaaagattc ggcaagggta cttttctctc catagattca aaaatgatat
     4261 tggcgaaata tttatgaact ctctggcagg tatagatatg ggcagaattt tactcaattt
     4321 atcgaatgag ttgtttaagc aacttgatga tctcgaggtg cagcgtaatc ttcctcgtgc
     4381 ggagctatta cgggaagcgg tagatcaata cctggtaaat caatcgcaaa cagcaagagc
     4441 cagtgctttt ggcatctggc aagggagtga ggaagatggt gtcgaatacc agcgtaagct
     4501 gcgcgaggaa tggtaatggc aaaaagattt ggctatttga tatcgatatt gttatcgatt
     4561 tgttcagtgt gaaaattgag gcaaaacagg cactggcagc atagcttgag gtggtaacgc
     4621 ggaataagag agatagtgtc gtcacaccct atgagctgta atccgggcga ccagcgccac
     4681 ccggaaaacg tcagagatta ctccccttca cccgggaaca ggaacgggtt gatggaactg
     4741 cgggcgtagc cttcttgctc cattcgcgcg tccagtacca gagaggcgag gtcatctgcc
     4801 acggcttcaa ctttcggttc tttttcctga taaagaatct tcaggtaagt gccgcagtca
     4861 tcgcagcttt cggctttaat cgcggcctgt tcgtcatcca gcgaccagta atgcagtttg
     4921 ccgctctgct cgcagttgct gcattttacg cgcactacgt gccattcggt ttcacacagg
     4981 ttgcagtgca ggtaacgcag accctgagtg gtgccaattt gcaccatgct ggacaccggc
     5041 atagagccac ataccgggca atattgacgt tgttcgccgt attcagcgcg ggctttgccg
     5101 gggatcagat tagccatctg cgcccagtag agcgacagcg cagcccagat aaacggcgct
     5161 ttatcgctgc tgacggacga gaaatcagag gcaaacagtg cgctggccat atcttccagc
     5221 tcctgagtcg atgccttctc cagattctca atcactgcca gcgccgggcc gctcatttca
     5281 ggtttcagct cagcaattag cgccatcagc agcttttgcc agtgcttatc acgcggcaga
     5341 acgtgaatat ccagcggggg cttgccttgt gcgctggctt ctttaatgcg cgtggtcaga
     5401 tccatctcca gcggatggtc gtacagcacc acttcctggg cgtgggcgat aagcgcagca
     5461 aagcgcaggt aatcacccag cggattattt tctgccagct cgcgcagacg ctcggcgcgg
     5521 cggttgtata aattcttgag ccgagggaac aataacggcg gaatcatatc cgccgtacgt
     5581 ttctcgctcg aacccagctc atcttgcggg attatgcgaa tactcattca gctttctttt
     5641 ccgttgtctt gcggacctca cggtaccagc gcgggtgatg tttcttcgcc caggcgctgg
     5701 taacccatcc ttccaccatg gcggtaatcg tgcctttcac ccaaagggcg gcgtagatat
     5761 gcaccatgat aaccacaatt aacgccactg cggcaaatga atgcagcatt aacgcgaatc
     5821 ggatcaccgg gattgagaaa gcaggcgcaa aataaggacg ccagataatc acgccgctca
     5881 ccagcagcag aaccaggaaa ataatcgccg cccagaaaac gcatttctga ccgaagttat
     5941 aacgcccggt gtcacctact tcctcgttga cgacgatctt acgaatattc ttcgcccaaa
     6001 agatatcatc ccgattgatt aggttgtgat gccagtaacg gaaaaacatg atgatgaacg
     6061 aggcaaacat aaccacgccg acaaacgggt gcagaattcg cgccagctgc ggtgtgccca
     6121 tgatttgcat caaccagttg aaggacggga acaaaaagcc cagcccgctc accgccgcca
     6181 ggatgaagca gaaggcggtg atccagtggt tgatacgttc cggcgcggtg tagcgcacga
     6241 tggtgtcacg tcgtttcatt tgcgctcctc gtctttctct tcgtgcagat tattctcttc
     6301 ctcatccgca cggttcggac cgacacccac gtagtggaag atactggctg cgaaggtagc
     6361 cgcaaagcca acagctgcga gcggtttcca gatgcctttc cagaatttca cggtttcgct
     6421 gatttccggg ttctccggca agccatgata cagatttggc ttgtcagcat ggtgcagcac
     6481 gtacataacg tgtgtaccac caacgcctgc cggatcgtac agacccgcat tgtcgtaacc
     6541 gcgagttttc agctccgcca cgcgctcgct cgccagcgtt ttcatcgact ctttcgtacc
     6601 gaagtgaatc gcgcccgttg ggcaggtctt cacgcaggcc ggttcttgcc caaccaccac
     6661 gcggtcaacg cacagcgtac atttgtagac gcggttgtct tccgggttga ggcgcggaat
     6721 gtcgaacgga cagcccgcaa tgcaataacc gcagccgatg cactgctcgg actggaagtc
     6781 gacgataccg ttggcatact gaataattgc tccttccgcc gggcacgctt tcaggcagcc
     6841 tggatcggaa cagtgcatac agccgtcttt gcggatcagc cattccagtt tgtcgttctg
     6901 ttccacttcc gagaagcgca tcaccgtcca cgatttggca cttaaatcat tggggttgtc
     6961 gtacacccca atgttattgc cgacggtatc gcgaatgtcg ttccactctg aacacgccac
     7021 ctgacaggct ttacagccga tacaggtggt gacgtcgatg agtttcgcca cttcttcctg
     7081 gaagtcccgc gcctgaggcg cgggggtcag accgttagtc gcggaacgac gaatgatatc
     7141 ttgagattga taagccatat gtcgtctccg ttacaccttt tccacattca caaggaagga
     7201 cttaaactct ggcgtctgcg tgttcgcatc accaacgaat ggcgttaacg tattggcaat
     7261 aaagcctttt ttcgcaacac cttcatagcc ccagtgaatc gggataccga tggtatcgat
     7321 atctttgccg ttcgctttca gcgtgcgaat acgtttggtc accaccgctt tggcttttat
     7381 atagccacgg ttggaggaga ctttcactgt atcgccctgg gcaatgccaa gtttattcgc
     7441 cagcgactca ccgatttcca caaactgctc tggttgcaaa atcgcgttca acagcgcgtg
     7501 tttggtccag tagtggaagt gctcggtcag acgataggtg gttccgacat acgggaactt
     7561 atcggcttta cccaatgctt cggcgtcgtc tttaaagata cgtgcagccg ggttcgagat
     7621 aacgtttgga tgcagcgggt tagttcccag cggcgtttca aacggctcgt agtgttccgg
     7681 gaacggacct tccgccatct tatcgagggc aaacagacgc cccatgcctt cctgctgcat
     7741 gataaacggc ccaacgccac tgcccggagg cgctgcgctg tagtccggaa tatcccagcc
     7801 ggtccactta gtgccgtccc atttcagcaa ctgacgcttc ggatcccacg ggttaccctg
     7861 cggatctgcg gaggcgcggt tatacagaat gcggcggtta agcggccatg cccatgccca
     7921 gcccagcgtg ttaccgaggc cagacggatc ggcgttatca cgacgcgcca tctggttgcc
     7981 ctctggcgtc cagctaccgg cgaaaatcca gcagccacag gaggtggtac cgtcatcgcg
     8041 cagttgggcg aacgagctaa gttgttggcc tttcttgacg ataaccgcac cggttgccgg
     8101 atcggtaata tcggccagcg ccttaccgtt gctctccatc gccacttctt ccgacttcgg
     8161 ctcatgcggg atggcgtagt tccaggtcat gttcagcacc tggtccgggt tcgcgccacc
     8221 ctgttcggca tacatcttgc gcaagcgcag gaagataccg gagaggatct cgccgtcagt
     8281 cagcgcaatc ccaggggcgt ccgcaccttt ccagtgccac tgcaaccagc gacctgagtt
     8341 aacgattgaa ccgttctctt ccgcgaagca ggtcgatggc agacggaaca cttcggtctg
     8401 gatcttcgac gagtcaactt cgttcagctc accgtggttc tgccagaagt tagaggtttc
     8461 agtgttcagc gggtcgatag tgacgaggaa cttcagtttc gacagacaac tgatcacttt
     8521 gtttttgttc gggaatgagg caacagggtt aaagccctgg cagatatagc cattgacctt
     8581 gccttctttc atcatctcga agtattgcag gacgtcgtag cctttatccc acttcggcaa
     8641 ccagtcaaag ccccagctat tttccgccgt cgctttatca ccaaagaagg ccttcatcat
     8701 agagacgaag aatttcgggt agttgcccca gtagtttacc tggccttcca gcagcggttt
     8761 tggcgtgttg gcggtaaggt aggtttgcag atcggtctgc ttctcgcttg gcagcgtcat
     8821 gtaacctggc aggctctgcg acagcagccc caggtccgtc agcccctgaa tattagagtg
     8881 accgcgcagg gcgttaacgc cgccgcctgc catccccatg ttgccgagca gcagctggat
     8941 catcgccatc gtacgaatgt tttgcgcacc aacggagtgt tgcgtccagc cgagggcata
     9001 caggaacgag gcagttttat cgtgagcact ggtttctgcg atgtattcgc agactttcag
     9061 gaacgcgtct tttggcgtac cacagatgtt ttcaaccaca tctggcgtgt agcgggaaac
     9121 gtgctgtttc agcaagttcc acacgcagcg cggatgttgc agcgtggtat cgcgtttggc
     9181 gaagccgttt tcgtccagtt cgtaagtcca ggaggattta tcgtacttgc gtttttccgc
     9241 gtcatagccg gtgaacaggc catcttcaaa gccgtaatcc tcacgcacga tcaggctggc
     9301 gttggtatag gcttcggtgt attcgcggtt gaatttttca ttgttcagca ggtacagcaa
     9361 tacgcctgac aggaaagcaa tgtcagtacc ggaacgaata ggggcatagt agtcagccac
     9421 cgccgccgta cgcgtaaagc gaggatcgat cacaatcagc ttcgcgccgt tgtgaatttt
     9481 ggcttccatc gcccagcgga acccgaccgg gtgagcttca gcggcgttac cgcccatcac
     9541 cacgacgagg ttggcgttct tgatgtcgac ccagtggttg gtcatcgcac cgcgaccaaa
     9601 tgttggagca agacttgcta ccgttggtcc gtgtcagaca cgcgcctggt tgtcgaccgc
     9661 gagcataccc agcgcgcggg agaatttttg cgttaaatag ccggtttcgt tactcgacgc
     9721 ggaagcacac agcatcccgg tggagagcca gcggttaacg gtcacgcctt cggcgttttg
     9781 cgcaatgtag ttagcatcgc ggtcttcttt catcagtttg gcgatgcgat caaacgcctc
     9841 ttcccaactg atttgctgcc atttatcaga acctggcgca cggtattccg gaaacttcag
     9901 acggctttcg gagtggatga aatccaccag gccagcgcct ttcggacaaa gtgcaccgcg
     9961 gttgaccggg tgatccggat cgccttcgat atggaagata gatgctttgg cgttttttgc
    10021 tccgtcaccg aggctgtaca tcaacagccc acagcctacg gaacaatagg tgcaggtatt
    10081 acgggtttcg cgggtgcgca gcagtttata ctgccgggtt tccgcgagtg ctacgctggg
    10141 ggcaaaaccc agtgccgccg ccgtggtgcc tgccataccg ccagcgcaga tcttaaagaa
    10201 ctgccttctg ctgacctgca tggattgctc cttgtttcga cattgtcacg tcccatttac
    10261 attcgcttgc tgcgtgtgca gggagtggga gttatttttc tttgcggaag gggccgcaaa
    10321 ggtccagaat tggctcaatt tccctccatc caggaagggt ttgtaacaga ataccataat
    10381 gttggtgtgt gtgttcttat ctggttaaga gaaagtgaaa aaaacacagc aaaaagaaat
    10441 cgaaaatgtg acaaatatca caggtgttcg tcaaattgag ttatggcggc gtgatgattt
    10501 acaacaccca cggttagatg aggtcgcgga agaagttccc gttgcgctgg tctacaacgg
    10561 catttcgcat gtggtgatga tggcgtcgcc caaagacctt gagtactttg cgctcggttt
    10621 ttcgctttcc gaggggataa tcgaaagtcc gcgcgatatc tttggcatgg atgtcgttcc
    10681 ttcctgtaat ggtcttgaag ttcaaattga gctttccagc cgccgcttta tggggttgaa
    10741 ggaacgccgc cgggcgctgg cgggacgtac gggctgtggc gtatgcggtg tagaacagct
    10801 taacgatatc gggaaaccgg tgcagccgct gccgttcacc cagacgtttg atctcaacaa
    10861 actggatgat gcattacgtc atctcaatga tttccagcca gtggggcaac tgactggttg
    10921 tactcacgcc gctgcatgga tgttgccatc tggcgaactg gtcggcgggc atgaagacgt
    10981 gggtcgccat gtggcgctgg acaaactgtt aggccgccgg tcacaagaag gggaaagctg
    11041 gcagcaaggt gcggtgctgg tttccagtcg tgccagttat gaaatggtgc aaaagtcggc
    11101 gatgtgtggc gtagagattt tgtttgcggt gtctgccgcg accacacttg ctgtagaagt
    11161 ggctgaacgc tgtaatttga cgctggtggg cttttgtaaa ccgggtcggg caacggttta
    11221 tacccatccg cagcgtttga gcaattaatt aaaataagtc ctg
//
LOCUS       DACBVI010000143        11241 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.144, whole genome shotgun sequence.
ACCESSION   DACBVI010000143 DACBVI010000000
VERSION     DACBVI010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.144"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(304..2571)
                     /gene="adiA"
                     /locus_tag="IU325_18365"
     CDS             complement(304..2571)
                     /gene="adiA"
                     /locus_tag="IU325_18365"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HAP5810968.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGITRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHIMCVKA"
     gene            complement(2770..3678)
                     /gene="melR"
                     /locus_tag="IU325_18370"
     CDS             complement(2770..3678)
                     /gene="melR"
                     /locus_tag="IU325_18370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HAP5810969.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3961..5316
                     /gene="melA"
                     /locus_tag="IU325_18375"
     CDS             3961..5316
                     /gene="melA"
                     /locus_tag="IU325_18375"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP5810970.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5431..6840
                     /gene="melB"
                     /locus_tag="IU325_18380"
     CDS             5431..6840
                     /gene="melB"
                     /locus_tag="IU325_18380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HAP5810971.1"
                     /translation="MTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLF
                     LVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEGTTQI
                     VFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTAGVTL
                     PFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHLTLKA
                     IVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAGAANL
                     VTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILLNVGT
                     ALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGY
                     VPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKV
                     PPEPVHADIPVGAVSDVKA"
     gene            complement(6979..7608)
                     /locus_tag="IU325_18385"
     CDS             complement(6979..7608)
                     /locus_tag="IU325_18385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HAP5810972.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATARRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGHKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(7731..9377)
                     /locus_tag="IU325_18390"
     CDS             complement(7731..9377)
                     /locus_tag="IU325_18390"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HAP5810973.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILQDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPEELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(9455..10795)
                     /locus_tag="IU325_18395"
     CDS             complement(9455..10795)
                     /locus_tag="IU325_18395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAP5810974.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
BASE COUNT         2665 a         2710 c         2951 g         2915 t
ORIGIN      
        1 ttctcgctaa agcaaagcga taccgggaaa tggttatata attgtatgat gaatataaac
       61 atgcataaaa ataacgagtt ttactaatat tacaaatttt tatagtaatt tctcatgtac
      121 atattttttt acaggctaaa aaaaggataa ataacaggca aaaaaaagcg cggggtggga
      181 ccgcgcaaaa agcatgacat tttttgtttg cagcaatttt tatgaaaacg ttgccatact
      241 taaaaggcct caagccaggc ggcgtaatgt tatttaacaa ttacgccttc agcggaatag
      301 tggttacgct ttcacacaca taatgtggta aataccgtca ataatttctg tcccttctgt
      361 ttcgtgttca aacccaggga aatgatggtc ccaggattgc agcgagcgta aataacttac
      421 ttgcggactg tttttatcgc cgaagttttc accagacagc agcatcggga ttcctggcgg
      481 atacgggata actgagtttg ccgcgatgcg tcctggcaga ttttcaatgg ataccagttc
      541 gacattgttg tcgacaatcg cgttgtacgc ttcacgtggg gtgatttccg ccaccggcag
      601 gccggaatag gcttcgttca accgtgcgcc agggttgttt tctttcagcc aggcaaacat
      661 ggtgtcaccc agatcgtgaa tccccatgtt cgcgtaagtg tcaggatatt gttcaacaag
      721 ttccggcatc acctgcgcca gcggtgtgtt ggcgtcatag tggcgtttga aggagcaaag
      781 ggtgttaacc agagtccccc atttcccacg ggttatgccc atagagaaca ggaacatgat
      841 ttggaagtca gtggtgcggg taggcacaat gccgtggcga ccaagccagg cagtgaccag
      901 cgccgccgga acaccggttt cttccagttc accatcttca cccattcccg gagcaaggat
      961 gctgacttta attgggtcga gcatactcca gttatccgga atatctttga agccgtgcca
     1021 gctttcgccc ggatgcatga cccagcagtc ctgaacggtg gtcagcagtt tggttggtgc
     1081 gtcagcaaag tcataggttt tgccggtttg tgggtcggtg acgacttctt tgttccacgg
     1141 tttgaagaac cagctaccgt cagcggtgaa ctctttatac agccgcgcca tcgcctgacg
     1201 gaaatcaacc gcttcgtcga tcacttcctg agtcagtgac aggccgctgt tgccgtccat
     1261 catcgacacc gccacgtcgt tggatgcgca gatggcatac agcggggagg tggtggcatg
     1321 catcatgtag gcctggttga agcgggagaa gttaatcgcc ccacgacctt cacgtacatg
     1381 aatataagag gcctgtgaca gcgcattcag cagtttgtgg gtggagtggg tggcgaaaac
     1441 ggtagggccg ttgtgatcgc caggttcgcc gcgcatggca tagtgatcgg catagatcgg
     1501 gttgaaacgt gcatagccgt accaggcttc gtcaaagtgc agacgatcgg aggttttttc
     1561 cagcagatcc tgcgcttctt tagcgttata acacacgccg tcataggtgc agttggtcac
     1621 cacgcagtaa gacggttttt gcccggcttt gtctttagtc agcgggcttt cactgatttt
     1681 cttctgcaag gtttcaggtt gcatttcctg cggatagatt ggcccgataa tgccgtagcg
     1741 gttgcggctt ggcaccatgt agaccggttt cgcgcctgtc agcatcaagc cttgttcgat
     1801 ggatttatgg cagttacggt caacgaccac gacatcgtta tcggtcatgc aagcctgcat
     1861 gatggtgcgg ttagagccgg aagtaccgac gactaccgac caggagcgat cggcaccaaa
     1921 tacgcgtgcg gcatattttt cgctttcgcc aaatgcgcca gtatggtcaa gcaaagaacc
     1981 gagggaagtg cgttcgatgc ccatgtcggt gcggaacagg ttttcaccat agtagtcatg
     2041 gtagaaacgt ccggcgggtg ttttggtaaa accaacgcca ccctggtggc ctggcgctgc
     2101 ccaggaatat tcatggatgt cgctatattt catcagcgcg ctgaacagtg gcggcaacag
     2161 ctgctggcgg tagcgggtca tcgcggcaac ggcgcgtccg gcgataaagt cggcggtatc
     2221 ttccagaatc caggcgaatt catcgacaag ctccagcaga tcccggtcca ttgcggcgag
     2281 ggctttttcc cgatcgccca acaggaagac cggcacgttt tgttggcgct catgaagctt
     2341 accgatcaat tgtctgacgt tttgatgttc gtccggatgt tccatttgat agctgaacat
     2401 caggcagtca atggcttcgt ttgaagagag aatggcaaaa ccatcatcaa aggaggtgga
     2461 tttaatcacg gtaacatttt gctggcttaa agcatctgcc agacgctcaa cggcgttacc
     2521 gacccaggtg tcttgatgga gaaactcgct ttcaacaatt aatactttca tcattgctta
     2581 cccggttatg aaggaatctt cgttgcgggc aagtatcttc cggccttttc gggctgtaaa
     2641 gcgcgtgaaa aacaaacatt tttgaaatat ttatttttat aaacaatatg ttatgtgatt
     2701 attttaaatt attttcactg cttattatcg acggctaaac tatttttttg gctgatactg
     2761 atatcgtctt tagccgggaa acgtctggcg gcgctgttgg ctaagtttgc ggtattgttg
     2821 cggtgacatg ccgacatatt tgccgaacgt gctgtaaaaa cgactacttg aacgaaagcc
     2881 tgccgtcagg gcaatatcga gaatactttt atcggtatcg ctcagtaacg cgcgaacgtg
     2941 gttgatgcgc atcgcggtta tgtactgttt catcgtcaat tgcatgaccc gctggaatat
     3001 ccccattgca tagttggcgt taagtttgac gtgctcagcc acatcgttga tggtcagcgc
     3061 ctgatcatag ttttcggcaa taaagcccag catctggcta acataaaatt gcgcatggcg
     3121 cgagacgctg tttttgtgtg tgcgcgaggt tttattgacc agaatcggtt cccagccaga
     3181 gaggctaaat cgcttgagca tcaggccaat ttcatcaatg gcgagctggc gaatttgctc
     3241 gttcggactg ttcaattcct gctgccagcg gcgcacttca aacgggctaa gttgctgtgt
     3301 ggccagtgat ttgatcacca tgccgtgagt gacgtggtta atcaggtctt tatccagcgg
     3361 ccaggagaga aacagatgca tcggcagatt aaaaatcgcc atgctctgac aggttccggt
     3421 atctgttagt tggtgcggtg tacaggccca gaacagcgtg atatgaccct gattgatatt
     3481 cactttttca ttgttgatca ggtattccac atcgccatcg aaaggcacat tcacttcgac
     3541 ctgaccatgc cagtggctgg tgggcatgat atgcggtgcg cgaaactcaa tctccatccg
     3601 ctggtattcc gaatacagcg acagcgggct gcgggtctgt ttttcgtcgc tgctgcacat
     3661 aaacgtatct gtattcatgg atggctctct ttcctggaat atcagaatta tggcaggagt
     3721 gagggaggat gactgcgagt gggagcacgg ttttcaccct cttcccacag cggcgagggg
     3781 agcgtccgag catcatgagg ccgaaaactc tgcttttcag gtaatttatt cccataaact
     3841 cagatttatt gatgcttcac gtaggatctg agtttatggg aatgctcaac ctggaagccg
     3901 gaggttttct gcagattcgc ctgccatgat gaagttattc aagcaagcca ggagatctgc
     3961 atgatgtctg cacccaaaat tacatttatc ggcgctggtt cgacgatttt cgttaaaaat
     4021 attcttggtg atgtgttcca tcgcgaggcg ctgaaaacgg cgcatattgc cctgatggac
     4081 attgatccca ctcgcctgga agagtcgcat attgtggtgc gcaagctgat ggattcagca
     4141 ggggccagcg gcaaaatcac ctgccacacc caacagaaag aagccttaca ggatgccgat
     4201 tttgtcgtgg tggcatttca gattggcggt tatgaacctt gcacggtgac tgatttcgag
     4261 gtctgtaagc ggcatggtct ggaacaaacc attgccgata cgttggggcc gggcggtatt
     4321 atgcgcgcgc tacgtaccat tccgcatctg tggcaaattt gcgaggacat gacggaagtc
     4381 tgccccgatg ccaccatgct caactacgtt aacccaatgg cgatgaatac ctgggcgatg
     4441 tatgcccgct atccgcatat caaacaggta gggctgtgcc attcggtgca gggaacggcg
     4501 gaagagctgg cgcgtgacct caatatcgac ccggctacgc tgcgttaccg ttgcgcaggt
     4561 atcaaccata tggcgtttta cctggaactg gagcgcaaaa ccgccgacgg cagttacgtg
     4621 aatctctacc cggaactgct ggcggcttat gaagcagggc aggcaccgaa gccgaatatt
     4681 catggcaata ctcgctgcca gaatattgtg cgctatgaaa tgttcaaaaa gctgggctac
     4741 ttcgtcacgg aatcgtcaga acattttgct gagtacacac cgtggtttat taagccaggt
     4801 cgtgaggatt tgattgagcg ttataaagta ccgctggatg agtacccgaa acgctgcgtc
     4861 gagcagctgg cgaactggca taaagagctg gaggagtata aaaacgcctc ccggattgat
     4921 attaaaccgt cacgggaata tgccagcaca atcatgaacg ctatctggac tggcgagccg
     4981 agtgtgattt acggcaacgt ccgtaacgat ggtttgattg ataacctgcc acaaggatgt
     5041 tgcgtggaag tagcctgtct ggttgatgct aatggcattc agccgaccaa agtcggtacg
     5101 ctaccttcgc atctggccgc tctgatgcaa accaacatca acgtacagac gctgctgacc
     5161 gaagccattc ttacggaaaa tcgcgaccgt gtttaccacg ccgcgatgat ggacccgcat
     5221 actgccgccg tgctgggcat tgacgaaata tatgctcttg ttgacgactt gattgctgcc
     5281 cacggcgact ggctgcctgg ctggttgcac cgttaaaacg cgactaaacg ctactgcgcc
     5341 gggggattta ttccggcgca cacttctgac gataccaata acagaaggcg ggcgttggta
     5401 acaacgaccc gataccctat gagcatttca atgactacaa aactcagtta tggatttgga
     5461 gcgttcggga aggattttgc gatcggcatt gtgtatatgt acctcatgta ttactacacc
     5521 gatgtcgtcg ggctgtctgt gggtttggtc ggtactttgt ttctggtggc gaggatctgg
     5581 gatgctatta acgatccgat tatgggatgg attgtaaatg ctacgcgatc gcgatggggt
     5641 aagttcaaac cctggatcct gatcggtacg ttggcaaact ctgtaatctt atttctcctc
     5701 tttagtgcgc atctgtttga aggtactact cagattgtct ttgtttgcgt gacctacatc
     5761 ctctggggca tgacttacac cattatggat attcccttct ggtcgctggt tccaaccata
     5821 acgctcgata aacgtgagcg cgaacaactg gttccttatc cgcgtttttt tgccagtctg
     5881 gcgggctttg ttacggcagg tgtgacgcta ccatttgtta attatgtcgg cggtggcgat
     5941 cggggatttg gctttcagat gttcactctg gtactgatcg ccttttttat tgtttcaacc
     6001 atcatcactc tgcgcaatgt gcatgaagtc ttttcgtcag acaatcaacc gtctgctgaa
     6061 ggaagccatc tgacacttaa agccatcgtt gcgcttattt ataaaaacga tcagctttca
     6121 tgcctcttgg gtatggctct tgcttataat gtagccagca atattattac cggctttgct
     6181 atctattatt tctcatatgt tatcggtgat gcggatttgt tcccctatta tctgtcgtat
     6241 gcgggagctg ctaatctggt gacgttagta ttcttcccac gcttagttaa atcattatcc
     6301 cgacgcattt tatgggccgg agcatctatt cttccggtgt taagctgtgg tgttctcctg
     6361 ttaatggcat taatgagcta tcacaacgtc gtcctcattg tgattgcggg tatcttgctg
     6421 aatgtgggaa cggcgctttt ctgggtatta caggtcatca tggtggcaga taccgttgat
     6481 tacggtgaat ataaactgca cgtacgctgt gaaagcatcg cttactccgt gcagactatg
     6541 gtggtgaagg gcggttcagc ctttgcggct tttttcattg cggttgtgtt agggatgatt
     6601 ggctatgtac cgaatgttga acagtctacg caagccctat taggtatgca gtttattatg
     6661 attgctctac cgactctgtt tttcatggta acgctgattc tctacttccg tttctatcgc
     6721 ctcaatggtg acacgctgcg caggatccag atccatctgc tggataaata tcgcaaagta
     6781 ccgcccgagc ctgttcatgc tgatattccg gtcggtgcag tgagtgatgt gaaagcctga
     6841 cgtggacaat agctaacgaa aatggcctga tgcgatatgt ttatcaggcc atgtcttaat
     6901 gtaatgcttt ggatttgcaa attttgtaga ccggataagg aattcacgcc gcatccggcg
     6961 tcaacaaagc gcacgttgtt atccggttat caagccaaag cgccgtagct ggcggcaatg
     7021 aaagcgagcg agaaatatca ccgtcgttaa tgcgccaagc agcgcgcaga acatatcgga
     7081 ttgcgtgtcc cactggtcgc cctgggtgcc gagaaaatca tccgctccct ggcccattgc
     7141 cagcgccgcc caccactcga ttaattcata catcgcgctt atcgccagcg ccacgcagca
     7201 gaccagaaac gccaccatct tatgtccgcg aacgtacata ccgcgcacaa ggatctctcg
     7261 tgccaccagt gcaggcacca gcccctggaa aaaatgcccc agtttgtcat acggattacg
     7321 gctcaacccc agccattcct gcacctcaaa accaacgggg actttcgcgt aggtgtattg
     7381 tccgccgacc atcaggatga tggcgtgaaa gaaaatgagc gtatagagca gcggcgttaa
     7441 cggataacgt ctggcggtgg caagcagtag cggcacgaca ataatcaccg gtgtcacttc
     7501 catcagccag gtgagtttgt catgggccga aatgccggta taaatcagga ttaacgttag
     7561 cgccagtgcg ccggtgttaa gaattaacgg cttgagtgtg cgggtcatgg tgagttcgca
     7621 agtcagggaa aatactgact attcaccagc aagcgtgaaa ttacaatcgc aaaagaggtg
     7681 gcccaggggg atcacctggc agcatgctgc caggcgctgg gccgaagagg ttacttagtg
     7741 cagttcgcgc actgtttgtt gacgatttgc tggaagaagt cgttaccttt gtcatcgacc
     7801 aggataaacg ccgggaaatc ttctacttcg attttccaga tagcttccat acccagctcc
     7861 ggataagcga cgcattccag atgcttgatg ctctgctgcg ccagtaccgc cgccggaccg
     7921 ccgatgctac cgaggtagaa gccgccgtgt ttatgacacg cgtcggtaac ctgctggctg
     7981 cggttgcctt tcgccagcat gatcatgctg ccgccgtggg attgcagcag atccacgtag
     8041 gagtccatac gacctgcggt ggttgggcca agtgaacctg atggataacc ggcaggggtt
     8101 ttcgccggac ccgcgtagta gatcgggtga tctttgatgt actgcggcag ttctttaccg
     8161 gcgtcaatca gctctttcag cttggcgtgt gcaatatctc gacccacaat aatggtgccg
     8221 gtgagcgaca agcgggtgga taccgggtat tgcgaaagct gggcgaggat ctctttcatc
     8281 gggcggttaa ggtcaacttt caccgcttcg ccttcaccgg cctggcgcag ttcttccgga
     8341 atgtactggc ctgggttgtg ttccagtttt tcgatccaga taccttcgcg gttgattttc
     8401 gctttaatgt tacggtcagc ggagcaggag acgcccatgc ctaccgggca ggatgcaccg
     8461 tgacgtggca gacggataac gcgaatgtcg tgcgcgaagt atttaccacc aaactgcgcg
     8521 ccaagaccga gtttctgggc ctcttccagc agttcctgtt ccagctggac atcgcggaac
     8581 gcctgaccat gttcgttccc ttccgtcggc agttcatcgt aatagtgagc gcttgctaac
     8641 ttgacggttt tcaggttggt ttccgcagac gtaccgccaa tcacaaacgc gatatggtac
     8701 ggcgggcagg ctgcagtacc gagggtacgc attttctcaa cgaggaagtt tttcagtttg
     8761 ccgggagtca gcagggcttt ggtttcctgg tagagatagg ttttgttggc agagccgccg
     8821 cctttagcga cacagaggaa tttgtactca tcgccatcta ccgcgtacag gtcgatttgc
     8881 gcaggcaggt tagtgccggt gttgacctct ttgtacatgt ccagcgccgc attctgtgaa
     8941 tagcgcaggt tgtcttcgat ataggtgtta tagacgcctt tcgacagcgc ttcttcatca
     9001 ccgccaccgg tccacacgcg ctggcctttt ttaccgacga tgatcgcagt gccggtgtcc
     9061 tggcaggtcg gcagcacgcc tttggcggcg atttcggagt ttcttaagaa ttgcagcgcc
     9121 acgtacttgt cgttttcgct ggcttctgga tcttgaagaa tagccgcgac ctgtttctgg
     9181 tgtgccgggc ggagcatgaa agaagcgtcg tgaaaggctt gctgcgccag cagagtcagg
     9241 gcttctggtt ccactttcag gattgtttcg ccgtcgaagt cggcaacgct aacgtaatcg
     9301 gaagtgagta gatagtattc ggtattgtct ttccccatcg ggaaaggtgc ctggtagata
     9361 aagggtttgt ttgacatagc ttccagcctg taactctgta tttgttattc gaaaatggcg
     9421 tgccgcgtag tcacgggcgg cacgcaaagt gcatttataa gaacccgtac atcgcggcga
     9481 agatccagcc gaagacgcac gatacgctca caccaatcaa ccccggcaga ataaagctgt
     9541 ggttgatgac gaagcgaccg atgtgggtgg tgccggaacg gtcaaactga atcgctgcca
     9601 gatcgctcgg ataagtcggc aggatgtaat aaccgtagca agccggtgct gaagccacga
     9661 tgtatgccgg atcaacgccg atcgccagcg cgaccggaac aatcgccgcc agcgccgcag
     9721 cctgagagtt tacaaacttg gaaaccagca gcagaacaat ggcataggcc cacggatact
     9781 ctttcaccat ttcacccagt acgccctgaa tttcagacat atgcgcaccg aacatggttt
     9841 cagccatcca tgcgataccg tacaccgcca cgatggcgat cataccggaa cggaagactt
     9901 cgttttttga gatagacgcg ggattggttt tggtcaggat aataatcagc gccccggtaa
     9961 gcagcataaa catctgaata accagtacca tcgacagcgg tttgccaccg aaggatggac
    10021 gcaggtccga atcagcacca agaagtgcga ctacagcgat tgccccgagg aagatccaca
    10081 ttgcaagcca gttgcttttc ggcagttttt tatcgagcag cgtcgcggta tcaccgtaaa
    10141 catactcacg gttttccggt acggagatga atttctggaa ctcttcgtct ttatccagat
    10201 ctttaccgcg gaaccagctg aagataccga tcgccaggat accgattaac gtcgatggaa
    10261 tggtgattgc cagcaggtcg aggaactcaa gatggcgacc atcaaaggtg acattaccca
    10321 gcatcgcaac cagagacacg actgcaaccg acaccggact ggcgataatc cccatctgtg
    10381 caccgataga acttgccgcc atcgggcgtt ccggacggat gttgttctta atggcgacgt
    10441 cgtagatgat cggcagaatg gtgtaaacca catgacccgt accgcaaaga atggtcagcg
    10501 tacaggtcac aaacggcgcg acaattgaga catatttcgg gttgcggcgc agcagcttct
    10561 cggcaatttg cagcatgaca tcaagaccgc ccgaagcttg caaggtcgcc gatgccgcca
    10621 ccaccgcaat gataaccagc atgacatcaa ccggtggttt acctggctga aggtggaaga
    10681 cgaagaccag aatgaccaga ccgataccgc ctaataaacc cagcgcgata ccaccctttc
    10741 tggcaccata aaacagacat atcagtatta tgataagttg gatagtaaat aacatgtgtg
    10801 aaccctcgcg ataatcctat ttaaattttt gctgaataga tcacagtcac gttctgtttt
    10861 gtatgaactg ttttcagagc ctgactaaat atccgtttgg tcgttaccgg ctttagcaaa
    10921 tacctcacag tgaatattgg ctgattaaag cagcggcaaa tttatgcact gtgataagcg
    10981 gctttttcag agagaaaaac tctctggcga tgggtttata gtttgtactg cgcagatgtt
    11041 aatgttttgt ttgctattat cttattgata tttatcggtc tattttttgt tctcagggca
    11101 gatatcatta ggtatataga ttaatctgat ctacccattt gtgggtaaaa atacacataa
    11161 tgcgggtgac ataatagtta attaactttt gttagcgttt tgaaattaaa aacaccgttc
    11221 atctgaagag atattaattt t
//
LOCUS       DACBVI010000144        11031 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.145, whole genome shotgun sequence.
ACCESSION   DACBVI010000144 DACBVI010000000
VERSION     DACBVI010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.145"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(96..692)
                     /gene="nrfG"
                     /locus_tag="IU325_18400"
     CDS             complement(96..692)
                     /gene="nrfG"
                     /locus_tag="IU325_18400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HAP5810975.1"
                     /translation="MKPPQIPVKMLTTLTILMVFLCVGSYLLSPKWLAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRTNPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(689..1072)
                     /gene="nrfF"
                     /locus_tag="IU325_18405"
     CDS             complement(689..1072)
                     /gene="nrfF"
                     /locus_tag="IU325_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HAP5810976.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQERALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(1065..2783)
                     /locus_tag="IU325_18410"
     CDS             complement(1065..2783)
                     /locus_tag="IU325_18410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAP5810977.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALGSLLRGEFDGASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFTLFALISLASLALYGW
                     RARDGGPAVHFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGPDRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            complement(2803..3759)
                     /gene="nrfD"
                     /locus_tag="IU325_18415"
     CDS             complement(2803..3759)
                     /gene="nrfD"
                     /locus_tag="IU325_18415"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HAP5810978.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLLLWLAKIFEHDLLALQQRWLPKLGFVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(3756..4427)
                     /gene="nrfC"
                     /locus_tag="IU325_18420"
     CDS             complement(3756..4427)
                     /gene="nrfC"
                     /locus_tag="IU325_18420"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HAP5810979.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(4424..4990)
                     /gene="nrfB"
                     /locus_tag="IU325_18425"
     CDS             complement(4424..4990)
                     /gene="nrfB"
                     /locus_tag="IU325_18425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HAP5810980.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(5035..6471)
                     /gene="nrfA"
                     /locus_tag="IU325_18430"
     CDS             complement(5035..6471)
                     /gene="nrfA"
                     /locus_tag="IU325_18430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HAP5810981.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQKAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6864..8822
                     /gene="acs"
                     /locus_tag="IU325_18435"
     CDS             6864..8822
                     /gene="acs"
                     /locus_tag="IU325_18435"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HAP5810982.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSIEHVV
                     VLKRTGGKIDWQEGRDLWWHDLIEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVISDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            9049..9363
                     /locus_tag="IU325_18440"
     CDS             9049..9363
                     /locus_tag="IU325_18440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HAP5810983.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            9360..11009
                     /gene="actP"
                     /locus_tag="IU325_18445"
     CDS             9360..11009
                     /gene="actP"
                     /locus_tag="IU325_18445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HAP5810984.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLLTAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSVTDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
BASE COUNT         2549 a         3091 c         2934 g         2457 t
ORIGIN      
        1 aatctgcaat aatactgatt ggtgattatt tttggcatat gttcattcga ttatttttta
       61 tgcagctcaa taacataatc tccatcacca gatgattaat ctgatctccg ctgcaacaat
      121 ttcgccatat taatcgactc gaccagctgt gtcctgttga tccggggtga gttgagatcc
      181 atcacttttt gccatagttc gatggcttgc gcgtaattcg cctgcataaa cgcatcggaa
      241 gccagcagca tcagagcggt gatttcgtta gagtccagcg cgagggcttt gtcgatcatt
      301 gcgcgagtct gggcggtcat atgctggcta gcctggtaat aaagcaccgt cgccagcgcc
      361 gcatacagtt cagcgttctc accacgtagt tgcaacgcct gacggtaagc cagcagcgaa
      421 ttgctgtaat cgttttgcca cagataatac tcgcccagta acgcccattg ttcgctgttt
      481 tgtggattag tgcggatttt atcctgcaat gcctgaagct gcgcttccgg ggtttgctgg
      541 ctggcaaact gatgtagcgg atcgcgctga cgctgatact ccgcacgcac cgccagccat
      601 tttggactta gcagataact gccgacacag agaaatacca tcaatatcgt gagtgttgtc
      661 agcattttca ccggtatttg aggcggtttc atcgcttcgc cctcactcgc cagaggatca
      721 gtgccatcag cagtaacaac accactggca gagcccacaa taccagcgtc tgacccgtta
      781 acggtgggtt atagcgaaca aaatctccgt agcgttcggt catccagcca atgatttcga
      841 cttcactttt cccctccgcc accatgctgt aaacctgatg gcgcatacta actgccaccg
      901 gcgcattgga ttccagtaag ttctgattct ggcactgcgg acaacgtaac tggctggcaa
      961 tatttaacgc ccgttcctgt tgttgcggat tggcgaattg ccaggtgtct acaacctgag
     1021 cgcgcgcaaa acaggtaaat aacagcagta gcgtgagaag tcctttattc atcgcgcttc
     1081 ctcccccgcc atccgcttaa caatgcgccc gcaatcatca acaatcctcc cccccagatc
     1141 cagcgcacac cgctttgtac atacaaacga aaagcgtaac gatccggccc agttttctcc
     1201 cccataaccg cataccagtc atggatgccg ttccagcgaa ttgacggctc catcatttgc
     1261 tgacggcgtg cttcgtaaaa acgccgctcc ggcgtcagtt caccaatacg ttgctgatgg
     1321 tcaaacagtg ccactatcgc tttttcgctg gtgtaattgc ctctggcttg cagatcgagg
     1381 cgctcaaaac ggaaggtgta tcctgccagc gtcacctgct gacccggctg taaattcagg
     1441 ctgatctcct gacggctgac gctggagacc acgatccccg cggcaaataa cagcacgccc
     1501 gcatgagcta ccagcgccgg aagctgcacg cgtttgccag agacaaacgt cgccagcaca
     1561 atcaccacca gcatcaacag accaaacggt aacgtcgcgc ggttaaaata cggcgcgcca
     1621 acggagaggc gtccccagcc cagcaggccg taaatcatcg gataaagcgt tcccaccagc
     1681 acgatcaata gcactgcgca aaacagcaac agcgtagcga ggattaacat ttcccgcgat
     1741 aaccccgaaa aatgcaccgc cgggccaccg tcccgcgctc gccagccata cagagccaga
     1801 gacgcaaggc taatcagtgc aaacagggtg aacaacggca cggcgcggac gttatccagc
     1861 gcgaacgcat gaaccgaaac cagaatgcca gaacggacaa ttaaggtacc cagcagcgac
     1921 agcatcagag tgactatcgc cagtaacagc gaccagtggc ggaaaatccc ccgctggcgt
     1981 gtcagggaca aactgtgcag cagcgcagtg gcagaaagcc agggtaataa agaggcgttt
     2041 tccaccggat cccagaacca ccagccgccc cagcccagtt cgcaataggc ccaccaggaa
     2101 ccgaggatga tccccgccgt taatgcgctc cagccaggta gtgcccagcg ccagcaaatt
     2161 cgggcgctgg caccatcaaa ctcgccgcgc agtaaactcc ccagcgccac gctcgctgcc
     2221 accatcaaac cgccatagcc gagataaagc agcggcggat gaaagataag accgggatgt
     2281 tgcagcatcg gattgagatc gcggccttcg attgctggtg gaaatatccg cacgaaggga
     2341 tcggaccaca gcaccacaaa cagtagcagt gcggcgagca tgagagataa aacggctagc
     2401 gtcagcggaa atagcggatc ggtttgctgc cgataatgcc aggcaaacag cgcgctccag
     2461 gcggaaagca acagcaccca aagcagcagc gaaccttcat gaccgcccca caccgccgcc
     2521 agtttgagtt cccacgacag caggctgtag ctatgttggg cgacataaat gaccgagaaa
     2581 tcgctgatga gaaaacagta cgtcagtacg ccaaaggcga gcagcaggag cgcgaactgc
     2641 gccagaatgc cgatgcaagt gagtcgcatc atggcaggcc agcgcaaccg cactcccgcg
     2701 aaggccgtca acggggtcaa cacgttgacc ccgagactta acaacaacgc cagaaaaccg
     2761 acttcaggaa ggaatacgtc cagaaaccac ctctgttctg gcttacgcca ccgttaactg
     2821 cccggcgtag agaatgaaaa atcgcaacat caacacgccg accaggctgg ccccacacgc
     2881 cgccagcacg gcaggaatgc cggaactgcg attgacccac ggtttgagca acattggcac
     2941 aatcaacccc agcccggcga caccaagcca gaaccaccac gtccagaaac cgccgcccaa
     3001 tgccgccacc agcgcacgca ctttaccgtc atcgcccagc gccagaccga caaaaaacgc
     3061 caccagcagg aagatttcgc cccataccac agggatctcc atacggtgta caaagtgcgc
     3121 ttccgtggaa tgcgggttac tgcgttggcg tatcgccatg gcgatcagcg ccaccgccgc
     3181 accggacgag atgccggaga agaggaacag caccggcagg atcgggttat tgaggaacgg
     3241 atacgatttc agcgccgata gcagaaagcc ggtataagcc cccagcagca ccgccagcac
     3301 cagcatcaat gtttccagtc cgcgatgaac gggcgtcagc aggctcagaa ccttttgcac
     3361 gaaccccagc ttcggcaacc agcgttgttg cagggcaagc aaatcatgtt caaagatttt
     3421 cgccagccac agcaacagca ccaccatgta gagctgaaac agcatcaccc ccatcgacat
     3481 caccgaggta aaactgtagt ggaacatcag cttccagaag gtccacggtc ttgtcaggtg
     3541 gaagacgagg atcaacagac caaggatcac cgcgccaggc ccgacaatca gcgtggtgcg
     3601 caacaacgta ctgtctgcac cgcccgcctg cgggtagaag cgacgtaaca gcacggccag
     3661 tgtcaccaga ccggcagaaa taccaatcaa aaacaggtag atggcaatcg gccagtccca
     3721 caccagcgat tcaaaatgaa atgcggaagt ctgcgtcatt ggctcacctc cccgtattta
     3781 aacggtacgc ggtacagctt cggtttggtt cccagcgcca gcttgtagcg gtaagtgggc
     3841 ttctggcgca gcagttgcga aatctcactg ttgggatcgt ccagattgcc aaacgtcagc
     3901 gccttcgtcg ggcaagcctc aacgcacgcg ggcagcttac cggcctgcaa attggtctta
     3961 cggcagaaat cgcatttgtc cgctgtcttc gtgaccggat ggataaagcg cacgcggtac
     4021 ggacaggcgg cgatgcagta ctgacaaccg acgcagagat ccgggttaac atcgacaatc
     4081 ccgctagcgg catcacgaaa agacgcaccg gtcgggcaga cgtcaacgca cggcgcatga
     4141 tcgcagtgct ggcaagactt acggaagaag cgatatttca catcaggaaa ttcgccctgc
     4201 ggctcgctac gaataatcgt caggcgcgag acgccttccg gcaccttgtt cacttcccga
     4261 caagcatcca tacaggcggt acagccgata cataacgact cgtcatgcac catgccgtaa
     4321 cgcaccccat tgatattcaa cgttttcgcc accacgcgcc ctgcggtgcc gctgacggct
     4381 gccagcacgc cgacgccggt gagaaactgg cgacgtgacc aggtcatggc tgctccttaa
     4441 gcaacggaac agacgccggg ttaaagttcg gattggtgcg ctgatcgctg tggcaatcga
     4501 cgcaaatctt aatccgtcct ttgtcactta acgtctgcat cgtatcttgc tgtggatgca
     4561 gggaatggca gctggcgcac gccactttgg ttacgtggac atcgtgtggc cagaacgctt
     4621 tttgcaactg ttccggcaga tggcaggaca tacagacgct gttctgctct ccaaccttgt
     4681 acatcggctc gttaaagcgc atcacatctt tcaccccttc gcggtgttgc ggtgatggct
     4741 ggccgtgaca gttggtgcag gtgaccggca gtttgttatt cgggttgata acggaagcat
     4801 gtttgccatg cataccttcg gtatctggct tatgacagtc cagacaggcg gcatccgggt
     4861 tacgctgctg ggtaacttcg tagcgatcac cagatgcctg cgccgcaggg gtagcggtaa
     4921 ttccgttcag gctccacaac aggcccgacg ccagcacccc ggcagttaat aacgaacgta
     4981 atacgctcat attcactcca ttgtgttttc cgccccttgc gaggcggaac ggggttattg
     5041 gcttaacaga ccgtttttac gtgcctgctc ttcccactgc gggatcaccg ttttgatgaa
     5101 gtcctgcttc tcggccttga tttgttccat gttcaggcca atggccttct gggctttctc
     5161 tttggtcgag atatccggga tctggatttc atgggtgatg cctttggttg ccagcagtcg
     5221 cgccagttta gtgcgcgcat ccgccgcttt atccatcgcc gtaccgagca tcctcagacc
     5281 ttcttccggt gcgtgcatat gaatgccgtg ggaagcgatc gccagatccc aacgccactg
     5341 ggcatgacgg atatcgtcct gaatcggctt catttcggct tccgtcgcgc ctgcatccag
     5401 cgccgctttc gcttcgaagt gagcgtgaac cagttgatct tcaaccttga ttttcaggtc
     5461 gttaatcgac tgcttacgtt ccgcgaccac tttttgcaag gcagctttgt cctgggtatg
     5521 gcagttcgca caagtctggg cgaagttatc aaacggatta ccaattttat ggtcggtgta
     5581 gagtttgcct tcggcgttct gcacttttgg catatggcaa tcgatacagg tcacgttgtt
     5641 tttgccatga atgcccgctg tccaggtttc atattccggg tgctgcgctt tcagcattgg
     5701 cgttttcgac agggagttag tccagtcaga gaaggcaatt ttgtcgtaat actgctccat
     5761 attttcgact ttcatgccgt catcccacgg gaatttaacc gctttgtttt tgccgtcgaa
     5821 gtaatactcc acatggcact gaccgcaaac catcgattgc tggtcgaaac gtccggcttt
     5881 ctcaaaaggt ttaccaatgg cttccatcgc gcgagccgca tacggacggg aaagggttaa
     5941 ctccggtttg cctttggcga actctggtga ggcggtgtta tggcaatcgg cacaacctaa
     6001 gttgttgacg atttccgggc cgccgcgcgc ccattttccg tggaagtagc catcttcgcc
     6061 gtctttctgg atcagacgcg ccacatccgg gcttttacaa ctccagcatg ccatcggtag
     6121 cggaccatct tcagcgtttt tcggcgcacc ggtgcgcagg gtttcacgca catcggtcac
     6181 agcaaaggca tgtccacgcg gcttgttgta atcgcgcgag aagggatacc ccgcccacag
     6241 gatcaccagc cgtggatctt ccgccagggc gtcaacacgc tctgactgtt ccgaggtggc
     6301 tttccaggag agatattgat cgggatgttg cggggcaaag gtttcattct tcgcttcaac
     6361 agttacaggt tttgcgggag ctgccgtttg ttcagcgtga acagaagtga aaaagaaaaa
     6421 aggaatcaat aagctgaaga tacggcgtgc gtttattttt atccttgtca taggggcttc
     6481 atccgaattg cgccattgtt gcaatggcgg tttttattgt ttttcacgac agtaaccgca
     6541 cctacactgt catgacattg ctcgccccta tgtgtaacaa ataaccacac tgtgaatgtt
     6601 gtctttaatc aattgtaagt gcatgtaaaa taccacttta gagttagtca gtatcttcct
     6661 ctttttcaac agcatgcata actgcatgtt cctcaaagaa ttaatcaact tttgttgctg
     6721 accttcaaaa attaccctgc cgtttatttg cacaattcta cttttgcgtg atctgtcgcc
     6781 caaatactaa acaaaactgc caatacccct acatttaact cttatgccac atattattaa
     6841 catcctacaa ggagaacaaa agcatgagtc aaattcacaa acacaccatt cctgccaaca
     6901 tcgcagaccg ttgcctgata aaccctcagc agtacgaggc gatgtatcaa caatctatta
     6961 acgcaccaga taccttctgg ggcgaacagg gaaaaattct cgactggatc aaaccttacc
     7021 agaaggtgaa aaacacctcc tttgcccccg gtaatgtgtc cattaaatgg tacgaggacg
     7081 gcacgctgaa tctggcggca aactgccttg accgccatct gcaagaaaac ggcgatcgta
     7141 ccgccatcat ctgggaaggc gacgacgcca gccagagcaa acatatcagc tataaagagc
     7201 tgcaccgcga cgtctgccgc ttcgccaata ccctgctcga gctgggcatt aaaaaaggtg
     7261 atgtggtggc gatttatatg ccgatggtgc cggaagccgc ggtcgcgatg ctggcctgcg
     7321 cccgcattgg cgcggtgcat tcggtaattt tcggcggctt ctcgccggaa gccgttgccg
     7381 ggcgcattat cgattctaac tcacggttgg tgatcacttc cgacgaaggc gtgcgcgccg
     7441 ggcgtagtat tccgctgaag aaaaacgttg atgacgcgct gaaaaacccg aacgtcacca
     7501 gcattgaaca tgtggtggta ctgaagcgta ctggcgggaa aattgactgg caggaagggc
     7561 gcgatctgtg gtggcacgac ctgattgagc aagccagcga tcagcaccag gcggaagaga
     7621 tgaacgccga agatccgctg tttattctct acacctccgg ttctaccggg aagccgaaag
     7681 gcgtgctgca cactaccggc ggttatctgg tgtatgcggc gctgaccttt aaatatgtct
     7741 ttgattatca tccgggcgat atctactggt gcaccgctga tgtgggttgg gttaccggac
     7801 acagttattt gctgtacggc ccgttggcct gcggcgcgac cacgctgatg tttgaaggcg
     7861 taccgaactg gccgacgcct gcccgtatgg cgcaggtggt ggacaagcat caggtcaata
     7921 ttctctatac cgcgcccacg gcgatccgcg cgctgatggc ggaaggcgat aaagcgatcg
     7981 aaggcactga ccgttcgtcg ctgcgcattc tcggttccgt aggcgagcca atcaacccgg
     8041 aagcgtggga gtggtactgg aaaaaaatcg gcaacgagaa atgcccggtg gtcgatacct
     8101 ggtggcagac cgaaaccggc ggtttcatga tcaccccgct gcctggcgct accgagctga
     8161 aagccggttc ggcaacacgt ccgttcttcg gcgtgcaacc ggcgctggtc gataacgaag
     8221 gtaacccgct ggaaggggcc accgaaggca gtctggtgat ctccgactcc tggccgggtc
     8281 aggcgcgtac gctgtttggc gatcacgaac gttttgagca gacctacttc tccaccttca
     8341 aaaatatgta tttcagcggc gacggcgcgc gtcgcgatga agatggctat tactggatca
     8401 ccgggcgcgt ggacgacgtg ctgaacgtct ccggtcaccg tctgggaacg gcagagattg
     8461 agtcggcgct ggtggcacat ccgaagattg ccgaagccgc tgtcgtcggt attccgcaca
     8521 atattaaagg tcaggcgatc tacgcctacg tcacgcttaa tcacggggag gaaccgtcac
     8581 cagaactgta cgcagaagtc cgcaactggg tgcgtaaaga gattggcccg ctggcgacgc
     8641 cagacgtgct gcactggacc gattccctgc ctaaaacccg ctccggcaaa attatgcgcc
     8701 gtattctgcg caaaattgcg gcgggcgata ccagcaacct gggcgatacc tcgacgcttg
     8761 ccgatcctgg cgtggtcgag aagctgcttg aagagaagca ggctatcgcg atgccatcgt
     8821 aacccacaat tgccggatgc ggcgtttaac gccttatccg gtctacacgc ccctttcttt
     8881 atagtgggaa agggcaaata taaaaattcc cctaagtatt tcgcgttgca ggcaggaagc
     8941 aagcgagcat ctccccagga gcatagaaaa ctatgtgact gggggaggcg agtgcggctg
     9001 acgaacctgc ggcgtgaaag acgacgggga acctctggag aatctgtgat gaatggtacg
     9061 atttatcagc ggatagaaga caatgcgcat ttcagggagt tagtcgaaaa acggcaacgg
     9121 tttgccacca tcctgtcgat tattatgctg gcagtttata tcggctttat tttactgatc
     9181 gccttcgcgc ccggctggct gggcactccg ctgaatccga acaccagcgt cacacgcggt
     9241 attccgattg gtgttggggt aattgtgatc tcctttgttc tcactggtat ctacatctgg
     9301 cgggcgaacg gcgaattcga ccgtcttaat aatgaagtcc tgcatgaggt acaagcatca
     9361 tgaaaagagt tctgacggcg cttgccgcca cactcccttt cgcagctaac gccgcggatg
     9421 ctattagcgg ggccgtagag cgccagccaa caaactggca ggcgattatt atgttcctga
     9481 ttttcgtcgt gtttacgctc ggtattacct actgggcgtc aaaacgcgta cgttcgcgta
     9541 atgactacta cacggcaggc ggcaatatca ccggttttca gaacgggctg gcgattgccg
     9601 gggactatat gtccgccgcc tcattcttgg ggatctccgc gctggtgttt acctccggct
     9661 atgacgggct gatctactcg ttgggcttcc tggtgggctg gccaatcatt ctgttcctga
     9721 ttgccgaacg tctgcgtaac ctgggacgct acacctttgc cgatgtagcc tcttaccgcc
     9781 tgaaacaagg gccgattcgt attctttcgg cctgtggttc tctggtggtg gtggcgcttt
     9841 accttatcgc tcagatggta ggcgcaggta aactgatcga gctgctgttt ggccttaact
     9901 atcacattgc ggtagtgctg gtcggcgtgc tgatgatgat gtacgtcctg ttcggcggca
     9961 tgctggcgac cacctgggta cagattatca aagccgtgct gttgctgttc ggtgccagct
    10021 ttatggcctt tatggtgatg aaacacgtcg gctttagctt caacaatctg ttcagcgaag
    10081 cgatggcggt acacccgaag ggtgtcgaca tcatgaagcc aggcgggctg gtgaaagatc
    10141 cgatctcagc actgtcgctg gggctcggac tgatgtttgg tacggctggc ttgccgcaca
    10201 ttctgatgcg cttctttacg gtcagcgatg cccgcgaagc gcgtaagagc gtgttctacg
    10261 ctaccggatt tatgggctac ttctatattc tgacctttat tatcggcttc ggcgcgatca
    10321 tgctggttgg tgcgaatccg gaatataaag acgcggcggg ccatctgatt ggtggtaaca
    10381 acatggcggc agttcacctg gcgaatgcag tgggtggcaa cctgttcctc ggctttattt
    10441 cagcggttgc tttcgccacc attcttgcgg tggttgcggg tctgacgctg gcgggcgcat
    10501 cggcggtgtc tcacgacctg tacgctaacg tcttcaaaaa aggcgcgact gaacgtgaag
    10561 agctgcgggt atcgaaaatc accgtcctga ttttgggcgt gattgccatt atcctcggcg
    10621 tgctgtttga gaatcagaac atcgccttta tggtggggct ggcgtttgcc atcgcagcga
    10681 gctgtaactt cccaatcatt ctgctttcca tgtactggtc aaaactgacc acgcgtggcg
    10741 caatgatggg cggctggctg gggctgctta ccgcagtggt actgatgatc ctcggcccga
    10801 cgatttgggt acagatcctc ggtcacgaaa aagccatctt cccgtatgaa tacccggcgc
    10861 tgttctctat cagcgtggca ttcctcggca tctggttctt ctcggtaacc gataactcag
    10921 cggaaggcgc gcgtgagcgt gaactgttcc gtgcgcagtt tatccgctcc cagaccggct
    10981 ttggcgttga gcaaggccgc gcgcattaat ctctcccctt ccccggtcgc c
//
LOCUS       DACBVI010000145        10933 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.146, whole genome shotgun sequence.
ACCESSION   DACBVI010000145 DACBVI010000000
VERSION     DACBVI010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.146"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            140..1354
                     /locus_tag="IU325_18450"
     CDS             140..1354
                     /locus_tag="IU325_18450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HAP5810985.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDANRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFDEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            1421..2704
                     /locus_tag="IU325_18455"
     CDS             1421..2704
                     /locus_tag="IU325_18455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5810986.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRSELGLSATEFGFLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRAMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            2957..3874
                     /gene="oxyR"
                     /locus_tag="IU325_18460"
     CDS             2957..3874
                     /gene="oxyR"
                     /locus_tag="IU325_18460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HAP5810987.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPSERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(3857..5257)
                     /locus_tag="IU325_18465"
     CDS             complement(3857..5257)
                     /locus_tag="IU325_18465"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HAP5810988.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            5589..6239
                     /gene="fabR"
                     /locus_tag="IU325_18470"
     CDS             5589..6239
                     /gene="fabR"
                     /locus_tag="IU325_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HAP5810989.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            6239..6598
                     /locus_tag="IU325_18475"
     CDS             6239..6598
                     /locus_tag="IU325_18475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HAP5810990.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(6638..7738)
                     /gene="trmA"
                     /locus_tag="IU325_18480"
     CDS             complement(6638..7738)
                     /gene="trmA"
                     /locus_tag="IU325_18480"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HAP5810991.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDGETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      7852..8042
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8107..9951
                     /gene="btuB"
                     /locus_tag="IU325_18485"
     CDS             8107..9951
                     /gene="btuB"
                     /locus_tag="IU325_18485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HAP5810992.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGEMIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVTVGHGNIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLNPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTLYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            9896..10753
                     /gene="murI"
                     /locus_tag="IU325_18490"
     CDS             9896..10753
                     /gene="murI"
                     /locus_tag="IU325_18490"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP5810993.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIITAVQKRYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLD"
BASE COUNT         2514 a         2594 c         2927 g         2898 t
ORIGIN      
        1 ttgaggatat tcttgttcct cggcggaaat ttttattttt aatgtgatca cggttgaata
       61 atcgcccttt ctctcttgta tggtagtagg cgtaaaatta agtttgaagc gtctatttac
      121 aacacaggag attgcaataa tgaaaattat tgctgcagat gtttttgtta cttgcccagg
      181 gcgtaatttc gttacgctta aaataaccac tgaaagcgga ctttgtggat tgggagatgc
      241 cacattaaat ggtcgcgaat tgtcggtcgc ttcttatctg aaagatcatc tttgtccaca
      301 attaattggc cgcgatgcca accgtatcga agatatctgg caattctttt ataaaggtgc
      361 ttactggcga cgtggccccg tcacgatgtc agccatttct gccattgata tggcgttatg
      421 ggatatcaaa gctaaagcgg caaatatgcc attgtatcaa ctgcttggtg gtgcttctcg
      481 tgaaggcgtg atggtttatt gccacaccac cgggcgtacg attgatgaag tccttgaaga
      541 ctatgcaaag caccagcaga tgggctttaa ggcgattcgt gtgcagtgcg gtgtaccggg
      601 tatgcaaacc acttatggcc tggcgaaggg caaaggactg gcgtatgaac cggcaaccaa
      661 aggcctgtgg ccagaagagc agttgtggtc ttcagaaaag tatctcgatt ttacgccgaa
      721 gttattcgaa gcggtgcgta ataagttcgg ttttaacgaa catctgttgc atgacatgca
      781 ccatcgcctg acccccatcg aagcggcgcg ctttgggaag agcattgaag attaccgcct
      841 gttctggatg gaagatccga cgcctgccga gaatcaggaa tgttttcgtc tgattcgtca
      901 gcataccgtg acgccgattg cggtcggtga agtgtttaac agtatctggg actgcaagca
      961 gttaatcgaa gagcagctga ttgactatat tcgcacgacg attacccatg caggcggcat
     1021 taccgggatg cgacggattg ctgattttgc atcgctttat caggtacgta ccggttcgca
     1081 tggcccttca gatttatcgc ctgtttgcca tgcggcggcg ctgcattttg acctctgggt
     1141 gccaaacttt ggcgttcagg agtacatggg ctattccgaa caaatgcttg aagtcttccc
     1201 gcatagctgg cgcttcgatg aaggttatat gcacccaggc gatgaaccgg ggctgggtat
     1261 ttctttcgat gagaaactgg cggcgaaata tccgtatgac ccggcgtatc ttcccgtcgc
     1321 acgactggaa gatggcacat tgtggaactg gtaataacgg ttcgcttaat tattttctga
     1381 atagtcatca aatcgattaa ttccgctcaa ggagctaaaa atggacgttg atgtgtcaac
     1441 cagtgttgcg ggtaataaac cgcaacggat tcgtcgtatt cagacagtga ccctggtttt
     1501 attatttatg gcggggatcg ttaattttct cgaccgttcg tcattgagcg tggcaggcga
     1561 agcgattcgt agcgagttag gattatcggc gacagagttt ggttttttgc tttctgcatt
     1621 ttctctgtct tatggttttt cacaacttcc ttccggtatt ttgctagacc gctttggccc
     1681 acgaatagtt ttaggcgcag gcttaatatt ctggtcatta atgcaggcat taacaggaat
     1741 ggttaattct tttagccact ttattttaat gcgtatcggt ctgggtattg gcgaagcgcc
     1801 atttatgcct gcgggagtaa agtcgatcac cgactggtat gcacaaaaag aacgcggcac
     1861 ggcgctgggg atttttaact catctaccgt tatcggtcag gctatcgcgc ctcctgctct
     1921 tgtgttgatg caactggcat ggggctggcg ggcaatgttc gtcatcatcg gcgtggcggg
     1981 gattctggtt gggatctgtt ggtacgcgtg gtatcgcaac cgggcgcagt ttgtcctgac
     2041 tgacgaagaa cggacgtatc tctccgcgcc ggttaaaccg cgtccacagc tgcaatttag
     2101 cgagtggctg gcgctgttta agcatcggac aacctggggg atgattttag gtttctctgg
     2161 cgtcaattat accggttggc tctacatcgc gtggttaccc ggttatttgc aggccgagca
     2221 aggtttcagt ctggcgaaaa ccggttgggt ggcggcgatt cctttcctcg cggcggcagt
     2281 cgggatgtgg gtaaacggta ttgttgtcga tcgactggcg aaaaaaggct acgacctggc
     2341 taagacgcgt aaaacggcta ttgtctgcgg tttgatgatg tcggcattag gcacgttgct
     2401 ggtcgtgcaa tcttcctcgc cagctcaggc tgtagcgttt atctcaatgg cgctattctg
     2461 cgtgcatttc gcgggaacgt ctgcatgggg actggtgcag gtgatggtgt cagaaacaaa
     2521 agtggcttcc atcgccggga ttcaaaactt tggcagtttt gtttttgctt cctttgctcc
     2581 gatcgtaacc ggttgggtag tggataccac acactcgttt aatctggcgt tggttatcgc
     2641 ggcctgtgtg acgttcaccg gggcgttgtg ttacttcttt attgtcaaag atcgcattga
     2701 gtaaccgtta ctatcaggct ctcttgttgt gggcctgtag aataaaaaaa aagcggatcc
     2761 tggagatccg caaaagttca cgttggcttt agttattcga gttgagaaac tctcgaaacg
     2821 ggcagtgact tcaagggtta aaagaggtgc cgctccgttt ctgtgagtaa ttatcagtca
     2881 gaatgcttga tagggataat cgttcgttgc tatgctacct atcgccgcga actatcgtgg
     2941 caatggagga tggataatga atattcgtga tcttgagtac ctggtggcat tggccgaaca
     3001 ccgccatttt cggcgtgcgg cagattcctg ccacgttagc cagccgacgc ttagcgggca
     3061 aattcgtaag ctggaagatg agttgggcgt gatgttgctg gagcggacca gccgtaaagt
     3121 gttgttcacc caggcgggaa tgctgctggt ggatcaggcg cgtaccgtac tgcgtgaggt
     3181 gaaagtcctt aaagagatgg caagccagca gggcgagacg atgtccggac cgctgcatat
     3241 tggtttgatt cccacggttg gaccgtacct gctaccgcat attatcccga tgctgcacca
     3301 gacctttcca aagctggaaa tgtatctgca tgaagcacag acccaccagt tactggcgca
     3361 actggacagc ggcaagctcg attgcgtgat cctcgcgctg gtgaaagaga gcgaagcatt
     3421 cattgaagtg ccattgtttg atgaaccaat gctgctggct atctatgaag atcacccgtg
     3481 ggcgaaccgc gaatgcgtac cgatggccga tctggcaggg gaaaaactgc tgatgctgga
     3541 agacggtcac tgtttgcgcg atcaggcaat gggtttctgc tttgaagccg gggcggatga
     3601 agatacacac ttccgcgcga ccagcctgga aacactgcgc aacatggtag cggcaggtag
     3661 cgggatcact ttattgcctg cgctggctgt gccgtcggag cgcaaacgcg atggggttgt
     3721 ttatctgccg tgcattaagc cggaaccacg ccgcactatt ggcctggttt atcgtcctgg
     3781 ctcaccgctg cgcagccgct atgagcagct ggcagaggcc atccgcgcaa gaatggatgg
     3841 ccatttcgat aaagtgttaa aacaggcggt ttaaaccgtt taacgcagct acccgatagg
     3901 cttccgccat cgtcgggtag ttaaaggtgg tgttgacgaa gtactcaata gtgttgccgc
     3961 cacctttctg ttccataatc gcctgaccga tatgaataat ttcggcagcg cgctcgccaa
     4021 agcagtgaat gcccagaatc tctttcgttt cccgatggaa caaaattttc agcgtgccca
     4081 cgttcatgcc gacgatttgt gcacgtgcca gatgtttaaa ctgggcgcgg cccacttcat
     4141 atggcacttt catcgcggtc agctgctgtt cggttttgcc cacagagctg atttccggga
     4201 tggtgtaaat gccggtaggg atatcttcaa tcagatgtgc ggtggcttca cctttcacca
     4261 gcgcctgcgc ggcaatgcgc ccctggtcat aggccgccga cgccaggctc ggatagccaa
     4321 tcacgtcgcc caccgcgtaa acgtgcggct gcgcggtctg atacatgctg ttgaccttca
     4381 gctgtccgcg actgtcagtt tccagcccaa tgttctgtaa cgccagcgaa tcggtattac
     4441 ccgtgcgacc gttggcatag agcagacagt cagctttcag tttttttccc gacttcagat
     4501 gcatgatcac accgtcgtca cagccttcga tcttctcgta ctcttcgttg tgacgaatca
     4561 ctacgccact gttccagaag tgataggaga gagaatctga catctcttga tcgagaaatg
     4621 ccagcaggcg atcgcgggtg ttgatcagat ccacttttac atccataccg cggaagatcg
     4681 acgcatattc acagccgatc actccagcac catagataag tacatggcgc ggttcgtggt
     4741 gcatgctaag aattgagtcg ctgtcgtaaa tgcgtggatg ggtgaaatca acatctgttg
     4801 gatgatatgg acgagagccg caagcaataa caaatttttc agcggttagt gtctcgacgc
     4861 tgccgtccgg gcaatccagc gccagcgtgt gctcgtcaac aaagcgagcg tttccctgca
     4921 atatttcaca gtgattacgt tcgtaaaatc cctgacgcat gcgcgtttgt tgattaatca
     4981 cgttatcggc atggttaagg atatcggcaa aagaagagcg gagcagtcgc gaatggtcgc
     5041 tgtaaagtgg gttttgattg aattctataa tgcggctgac ggcgtgacgg agagctttcg
     5101 acgggatggt gccccagtgg gtgcaaccgc cgccaacatt ttgataacgc tcgataactg
     5161 cgacgcgcgc accttgctta accaggccca ttgcagcgcc ttcgccgccg gggccggaac
     5221 ctatcactat ggcatcgtaa tcgtaggaat gtggcatggt agggcttacc tgttcttata
     5281 cataaaagca acagaatggt aacattttat cgcgggtaag ccaattgatc cccgtcattt
     5341 atctggctat atcgtgagcg gcctttgctt tgtctgtttc ttacttttga cctgaacgtt
     5401 ttattggatt tttatcgacg atactctccg tttaagcggc aggtttccga tgtacgtaaa
     5461 agaaccggcc aaagaattgc agtaaatatg ttttattgcg ttaccgttca ttcacaacac
     5521 tggagcaatc cagtatgttc attctctggt atagtgccag cagtactttt ggcaaggatt
     5581 cagacatcgt gatgggcgta agagcgcaac aaaaagaaaa aacccgccgt tcgctggtgg
     5641 aagccgcatt tagccaatta agtgctgaac gcagcttcgc cagcctgagt ttgcgtgaag
     5701 tggcgcgtga agcgggcatt gctcccacct ctttttatcg gcatttccgc gacgtagacg
     5761 aactgggtct gaccatggtt gatgagagcg gtttaatgct acgccaactc atgcgccagg
     5821 cgcgtcagcg tatcgccaaa ggcgggagtg tgatccgcac ctcggtctcc acatttatgg
     5881 agttcatcgg taataatcct aacgccttcc ggttattatt gcgggaacgc tccggcacct
     5941 ccgctgcgtt tcgtgctgcc gttgcgcgtg aaattcagca cttcattgcg gaacttgcgg
     6001 actatctgga actcgaaaac catatgccgc gtgcgtttac tgaagcgcaa gccgaagcaa
     6061 tggtgacaat tgtcttcagt gcgggtgccg aggcgttgga cgtcggcgtc gaacaacgtc
     6121 ggcaattaga agagcgactg gtactgcaac tgcgaatgat ttcgaaaggg gcttattact
     6181 ggtatcgccg tgaacaagag aaaaccgcaa ttattccggg aaatgtgaag gacgagtaat
     6241 gaaacaagca aatcaagata gaggtacgct gctgctggcg ttagttgctg gcttatcgat
     6301 taatggtact ttcgcagcgc tgtttagctc cattgtgcca ttttctgtat tcccgattat
     6361 ttccctggtg ctgacggttt attgcctgca tcaacgttat cttaatcgca ccatgccggt
     6421 aggcttgccg ggtctggctg ctgcctgttt tattcttggc gtactgctgt acagcacggt
     6481 agttcgtgcg gaatatccgg atatcggctc taacttcttc ccggcagtac tgtccgtcat
     6541 tatggtgttc tggattggcg cgaagatgcg taaccgtaag caggaagttg ctgagtaatc
     6601 ggtatcatgc cggatgctgg cgcacccggc atggatttta cttcgcggtc agtaatacgc
     6661 cgcactccat atggtgcgtg taggggaact gatcaaacag agccagacgt tcaaccttgt
     6721 gcgtctggct taatgtttcc agattcttgc ataacgtttc cggattgcag gagatataca
     6781 gaatacgtgg atacgcctgc accattttct cggtttcacc gtccagaccg ctgcgtggtg
     6841 ggtcgacaaa aatggtttcg cactgataac tctttaaatc gatcccttgc aggcggttaa
     6901 actcgcgtac gccattcatc gcctgagtaa attcttctgc cgccatacga ataatttgta
     6961 cgttatcaat atggttagcg gcaatgttgt attgcgcggc agcgaccgac ggcttagcga
     7021 tttcggtggc taatacccga tcaaaattgc gcgccagtgc taatgaaaag ttaccgttgc
     7081 cgcagtacag ctccagtaaa tcgccttttg agcctttggt gacgtccagc gcccattcca
     7141 gcatctgaat attcatcgcc gcgttcggct gggtaaagct gttttctacc tgacggtaga
     7201 tcatctcttt ccctgcgact ggcagacgtt catcgatgta atcctggtcc agcgcgattt
     7261 tggttttcgt tgcccgacca atcagatgca cattcagatt ctgcgctcgc agtgcatcgc
     7321 gcagggcttc cgcttcctga cgccactcat catccagctt cttatggtac agcagggaaa
     7381 ccaccgcctg attactcagc gtggtgaggt aatcaatctg gaacaacttg tggcgcagaa
     7441 cgggattatt acgcacaccc gcaatcatcg ccgtcatcaa ctggttgata agttcactgg
     7501 cggcggggaa gctatccacg cggatgcggc ttttggtttg ttgatcgaaa atgatgtgat
     7561 acaggtcatc gccatcgtgc cagatgcgga actccgcacg catccggtaa tgactgaccg
     7621 gcgagcgaaa cacttccgga accaggtcag aaaacggtgc catcatactt tgcaaacgta
     7681 ccactttttc ggctaactgc gcttcatact gttctgttgg aaggtgttcg ggggtcatga
     7741 tgtatcctga aaaattaaag tacgcggcga ttgtagggat tgctcatcag atgtccagat
     7801 cttgatgtat tcctatttgt gagctacgtc tggacagtaa cttgttacaa cctgtagcat
     7861 ccacttgccg gtcctgtgag ttaataggga atccagtgcg aatctggagc tgacgcgcag
     7921 cggtaaggaa aggtgcgatg attgcgttat gcggacactg ccatccggtg ggaagtcatc
     7981 atctcttagt atcttagata cccctccaag cccgaagacc tgccggccaa cgtcgcatct
     8041 ggttctcatc atcgcgtaat attgatgaaa cctgcggcat ccttcttcta ttgtggatgc
     8101 tttacaatga ttaaaaaagc ttcgctgctg acggcgtgtt ccgtcacagc cttttctgct
     8161 tgggcacagg ataccagccc ggatactctc gtcgttactg ctaaccgttt tgaacagccg
     8221 cgcagcactg tgcttgcacc aaccaccgtt gtgacccgtc aggatatcga ccgctggcag
     8281 tcgacctcgg tcaatgatgt gctgcgccgt cttccgggcg tcgatatcac ccaaaacggc
     8341 ggttcaggtc agctctcatc tatttttatt cgcggtacaa atgccagtca tgtgttggtg
     8401 ttaattgatg gcgtacgcct gaatctggca ggggtgagtg gttctgccga ccttagccag
     8461 ttccctattg cgcttgtcca gcgtgttgaa tatatccgtg ggccgcgctc cgccgtttat
     8521 ggttccgatg cgataggtgg ggtggtgaat atcatcacga cgcgcgatga acccggaacg
     8581 gaaatttcag cagggtgggg aagcaatagt tatcagaact atgatgtctc cacgcagcaa
     8641 caactggggg ataagacacg ggtaacgctg ttgggcgatt atgcccatac tcatggttat
     8701 gatgttgttg cctatgggaa taccggaacg caagcgcaga cagataacga tggtttttta
     8761 agtaaaacgc tttatggcgc gctggagcat aactttactg atgcctggag cggctttgtg
     8821 cgcggctatg gctatgataa ccgtaccaat tatgacgcgt attattctcc cggctcaccg
     8881 ttgctcgata cccgtaaact ctatagccaa agttgggacg ccgggctgcg ctataacggc
     8941 gaaatgatta aatcacaact cattaccagc tatagccata gcaaagatta caactacgat
     9001 ccccattatg gtcgttatga ttcgtccgcg acgctggatg agatgaagca gtacaccgtc
     9061 cagtgggcaa ataacgttac cgttggccac ggtaatattg gtgcgggcgt tgactggcag
     9121 aaacagacta cgacgccggg tactggttat gttgaggatg ggtatgatca acgtaacact
     9181 ggtatctatc tgaccggatt gcaacaagtc ggcgatttta cgtttgaagg cgcagcccgc
     9241 agcgacgata actcacagtt tggtcgtcat ggaacctggc aaacgagtgc cggttgggaa
     9301 ttcatcgaag gctatcgttt tatcgcttct tatggcacgt cgtacaaggc acctaatctt
     9361 gggcaactgt atggtttcta cggaaatccg aatctgaatc ctgagaaaag caaacagtgg
     9421 gaaggcgcgt ttgaaggctt aaccgctggg gtgaactggc gtatttccgg atatcgtaac
     9481 gatgtcagtg atttgatcga ttatgatgat cacaccctga aatattacaa cgaagggaaa
     9541 gcgcggatta agggcgtcga ggcgaccgcc aattttgata ccggaccact gacgcatact
     9601 gtgagttatg attatgtcga tgcgcgcaat gcgattactg acacgccgtt gttacgccgt
     9661 gctaaacagc aggtgaaata ccagctcgac tggcagttgt atgacttcga ctggggtatt
     9721 acttatcagt atttaggcac tctctatgat aaggattact catcttatcc ttatcaaacc
     9781 gttaaaatgg gtggtgtgag cttgtgggat cttgcggttg cgtatccggt cacctctcac
     9841 ctgacagttc gtggtaaaat agccaacctg ttcgacaaag attatgagac agtctatggc
     9901 taccaaactg caggacggga atacaccttg tctggcagct acaccttctg aaccacgtcc
     9961 caccgtgctg gtgtttgact ccggcgtcgg tgggttgtcg gtctatgacg agatccggca
    10021 tctcttaccg gatctccatt acatttatgc tttcgataac gtcgctttcc cgtatggcga
    10081 aaaaagcgaa gcgtttattg ttgagcgagt ggtggcaatt atcaccgcag tgcaaaaacg
    10141 ttatcccctt gcgctggctg tggtcgcttg caacactgcc agtaccgttt cacttcctgc
    10201 attacgcgaa aagttcgact tcccggttgt tggtgtcgtg ccggcgatta aacctgctgc
    10261 acgtctgacg gcaaatggca ttgtcggatt actggcaacc cgcggaacag ttaaacgttc
    10321 ttatactcat gagctgatcg cgcgtttcgc taatgaatgc cagatagaaa tgctgggctc
    10381 ggcagagatg gttgagttgg ctgaagcgaa gctgcatggc gaagatgttt ctctggatgc
    10441 gctaaaacgt atcttgcgtc cgtggttaag aatgaaagag ccgccagata ccgttgtact
    10501 ggggtgtacc catttccctc tactacaaga agaactgtta caggtgctgc cagagggaac
    10561 ccggctggtg gattctggcg cagcgattgc tcgccgaacg gcctggttgc tagagcatga
    10621 agccccggat gcaaaatctg ccgatgcgaa tattgccttt tgtatggcaa tgacgccaga
    10681 agctgaacaa ttattgcccg ttttacagcg ttacggcttc gaaacgctcg aaaaactggc
    10741 agttttagac tgatttggtt gaatgttgcg cggtcagaaa attattttaa atttcctctt
    10801 gtcaggcctg aataactccc tataatgcgc caccactgac acggaacaac ggcaaacacg
    10861 ccgccgggtc agcggggttc tcctgagaat tccggcagag aaagcaaaaa taaatgcttg
    10921 actctgtagc ggg
//
LOCUS       DACBVI010000146        10904 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.147, whole genome shotgun sequence.
ACCESSION   DACBVI010000146 DACBVI010000000
VERSION     DACBVI010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.147"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            87..746
                     /locus_tag="IU325_18495"
     CDS             87..746
                     /locus_tag="IU325_18495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001705611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP5810994.1"
                     /translation="MALLNCENNIIDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIP
                     VWQRSATPILTLPCLTPDVFRVAAQHSMMPAETESEKERTRALLFTVLSRFLDSKKFL
                     SLMMYMLRNCVSDSVYQIIESDIHKDWNLSMVASCLCLSPSLLKKKLKSENTSYSQII
                     TTCRMRYAVNELMMDGKNISQVSQSCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTV
                     A"
     gene            883..2220
                     /gene="adiC"
                     /locus_tag="IU325_18500"
     CDS             883..2220
                     /gene="adiC"
                     /locus_tag="IU325_18500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HAP5810995.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2324..3967
                     /gene="eptA"
                     /locus_tag="IU325_18505"
     CDS             2324..3967
                     /gene="eptA"
                     /locus_tag="IU325_18505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HAP5810996.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNETRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            3964..4632
                     /gene="basR"
                     /locus_tag="IU325_18510"
     CDS             3964..4632
                     /gene="basR"
                     /locus_tag="IU325_18510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HAP5810997.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4633..5733
                     /gene="pmrB"
                     /locus_tag="IU325_18515"
     CDS             4633..5733
                     /gene="pmrB"
                     /locus_tag="IU325_18515"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HAP5810998.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDSHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(5709..5798)
                     /gene="pmrR"
                     /locus_tag="IU325_18520"
     CDS             complement(5709..5798)
                     /gene="pmrR"
                     /locus_tag="IU325_18520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003826741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HAP5810999.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(5910..7412)
                     /gene="proP"
                     /locus_tag="IU325_18525"
     CDS             complement(5910..7412)
                     /gene="proP"
                     /locus_tag="IU325_18525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HAP5811000.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7676..8554)
                     /locus_tag="IU325_18530"
     CDS             complement(7676..8554)
                     /locus_tag="IU325_18530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811001.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLARVQLQAWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTIPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLV"
     gene            complement(8551..10779)
                     /gene="crfC"
                     /locus_tag="IU325_18535"
     CDS             complement(8551..10779)
                     /gene="crfC"
                     /locus_tag="IU325_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HAP5811002.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADFEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQIEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQIKMELS
                     ESGFRPGFHFPVFHGAVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQAVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2721 a         2852 c         2683 g         2648 t
ORIGIN      
        1 accaaccttg tattgtttta ttgactgaaa aagatgtctg gataagggtg aatgggaaag
       61 aacctattag ccttaaagct aaccatatgg cgttattaaa ttgtgaaaat aatattatcg
      121 acgtctcctc tcttaataac actttggttg ctcatattag tcacgacatc atcaaagatt
      181 acctccggtt tctgaataaa gatctctcgc aaataccagt atggcaacgt agcgctacgc
      241 ccatcctcac tctgccatgc ctgacgccag acgtctttcg cgttgccgcg caacatagca
      301 tgatgcccgc tgaaactgag tcagaaaagg aacgaacacg tgcattatta ttcactgtgc
      361 tatcccgttt tctcgacagt aaaaaattcc tttcactaat gatgtatatg ttacgtaatt
      421 gtgtaagtga cagcgtttat caaattattg aaagcgatat ccacaaagac tggaatctta
      481 gtatggtagc cagttgttta tgtcttagcc caagtctgtt aaagaaaaag ctgaaaagcg
      541 aaaacaccag ttatagccaa ataatcacca cctgccgcat gcgttatgcc gtaaatgaat
      601 taatgatgga cggtaaaaat atctctcagg tatcacagtc ctgcggctac aacagtacgt
      661 cgtactttat ttctgtcttt aaagacttct acggtatgac gcctctgcat tatgttagtc
      721 agcacagaga acgcactgtc gcctgatttt taaccttaac gaagagctat attaataacg
      781 gcatcagcga taacccggtc gataataatt caactatcga atgcaggcgt atgatatgac
      841 gtaatttatt gtcacgaagc tcgccttcgc aggagtttaa ttatgtcttc ggatgctgat
      901 gctcacaaag tgggcttaat ccccgtcacc ctgatggtgt cggggaatat tatggggtca
      961 ggtgtttttc tgttacctgc aaacctggcc tccactggcg ggattgccat ttatggatgg
     1021 ttggtgacga ttatcggtgc gctggggctc tcgatggtat acgccaaaat gtcgttcctc
     1081 gacccaagtc ctggtggttc ttacgcttac gcccgccgct gctttgggcc gtttctcggt
     1141 tatcaaacca acgtcctcta ctggctggcc tgctggatcg gcaatatcgc catggtggtc
     1201 attggcgtag gttatttaag ttacttcttc ccgattctga aagatccatt ggtattaacc
     1261 atcacctgcg tcgtggtgct gtggatcttc gtcctgctga acattgtcgg tccgaaaatg
     1321 atcacccgtg tgcaggccgt tgccaccgtg ctggcgctga tccccatcgt cgggattgcc
     1381 gtatttggct ggttctggtt ccgtggtgaa acctatatgg cggcatggaa cgtcagcggc
     1441 ctgggcacct tcggcgcgat tcaaagtacc cttaacgtta cgctgtggtc gttcatcggt
     1501 gtggaaagtg cctccgttgc cgcaggtgtg gtgaaaaacc cgaaacgcaa cgtccctatc
     1561 gccaccattg gtggggtatt gattgccgcc gtttgctatg tgctctccac caccgcgatt
     1621 atggggatga tccctaatgc cgcactgcgc gtttctgcct cgccattcgg tgatgccgca
     1681 cggatggcgt tgggtgacac cgccggggcc attgtttcct tctgcgcagc tgcgggttgc
     1741 ttaggttcac tgggcggctg gacgttgctg gcgggtcaaa cggcgaaagc cgctgccgat
     1801 gacggactgt tcccaccgat ttttgcccgt gtaaataaag cgggtacgcc agtggcaggg
     1861 ctgattatcg tcggtatttt gatgaccatc ttccagctca gtagcatttc gccaaacgcg
     1921 actaaagagt tcggtctggt gtcttccgtg tcggtcatct ttacactggt gccatatctt
     1981 tacacctgtg cggcgttact gctgctcggc cacggtcact ttggtaaagc acgcccggca
     2041 tatctggcag tgactaccat tgccttcctc tactgcatct gggccgtggt cgggtccggg
     2101 gcgaaagagg ttatgtggtc atttgtcacc ctgatggtaa tcaccgccat gtatgcgctg
     2161 aattacaacc ggctgcataa aaacccgtat cccttagatg caccaataag caaagattaa
     2221 ttccccttaa tcaagcaaac ataaaagcca accttaagaa cttaaggttg gcttaatttt
     2281 gctttgcgag catatgcgca ctttgttcga tggaaacacc gtgatgttga agcgcctact
     2341 aaaaagaccc tctttgaatt tactcgcctg gctattgttg gccgcttttt atatctctat
     2401 ctgcctgaat attgcctttt ttaaacaggt gttgcaggcg ctgccgctgg actcgctgca
     2461 taacgtactg gttttcttgt cgatgccggt cgtcgccttc agcgtgatta atattgtcct
     2521 gacactaagc tctttcttat ggcttaatcg accgctggcc tgcctgttta ttctggtcgg
     2581 cgcagctgca caatatttca taatgactta cggcatcgtc atcgaccgct cgatgatagc
     2641 caatattatt gataccactc cggcagaaag ttatgcgttg atgacaccgc aaatgttatt
     2701 aacgctggga ttcagcggcg tgcttgctgc gctgattgcc tgctggatta aaatcaaacc
     2761 tgccacctcg cgcctgcgca gtgttctttt ccgtggagcc aatattctgg tgtctgtact
     2821 gctgattttg ctggtcgccg cactgtttta taaagactac gcctcattgt tccgcaacaa
     2881 caaagagctg gtgaagtcct taagcccctc taacagcatt gttgccagct ggtcatggta
     2941 ctcccatcag cgactggcaa atctgccgct ggtgcgaatt ggtgaagatg cgcaccgcaa
     3001 cccattaatg cagaacgaga cacgtaaaaa tttgaccatc ctgattgtcg gcgaaacctc
     3061 gcgggcggag aacttctccc tcaatggcta cccgcgtgaa actaacccgc ggctggcgaa
     3121 agataacgtg gtctatttcc ctaataccgc atcttgcggc acggcaacgg cagtttcagt
     3181 accgtgcatg ttctcggata tgccgcgaga gcactacaaa gaagagctgg cacagcacca
     3241 ggaaggcgtg ctggatatca ttcagcgagc gggcatcaac gtgctgtgga atgacaacga
     3301 tggcggctgt aaaggtgcct gcgaccgcgt gcctcaccag aacgtcaccg cgctgaatct
     3361 gcctggtcag tgcatcaacg gcgaatgcta tgacgaagtg ctgttccacg ggctggaaga
     3421 gtacatcaat aacctgcagg gtgatggcgt gattgtctta cacaccatcg gcagccacgg
     3481 tccgacctat tacaaccgct atccgccgca gttcaggaaa tttaccccaa cctgcgacac
     3541 taacgagatc cagacctgta ccaaagagca actggtgaac acttacgaca acacgctggt
     3601 ttacgtcgac tatattgttg ataaagcgat taatctgctg aaagaacatc aggataaatt
     3661 taccaccagc ctggtttatc tttctgacca cggtgagtcg ttaggtgaaa atggcatcta
     3721 tctgcacggt ctgccttatg ccatcgcccc ggatagccaa aaacaggtgc caatgctgct
     3781 gtggctgtcg gaggattatc aaaaacggta tcaggttgac cagaactgcc tgcaaaaaca
     3841 ggcgcaaacg caacactatt cacaagataa tttattctcc acgctattgg gattaactgg
     3901 cgttgagacg aagtattacc aggctgcgga tgatattctg caaacttgca ggagagtgag
     3961 tgaatgaaaa ttctgattgt tgaagacgat acgctgttat tgcagggatt gattctggcg
     4021 gcacaaaccg aaggctacgc gtgcgatggc gtgacaaccg cgcggatggc ggaacaaagc
     4081 cttgaggccg gtcattacag cctggtggta ctggatttag ggttacccga cgaagatgga
     4141 ctgcattttc tcgcccgtat ccggcagaaa aaatacaccc tgccggtact gatcctcacc
     4201 gctcgcgata cgctgaccga caaaatcgcc gggctggatg tcggtgccga tgactatctg
     4261 gtgaagcctt ttgcgctgga agagttacac gcccgtatcc gcgccctgct acgacgccat
     4321 aataatcagg gcgaaagtga gctgattgtt ggcaatctga cgctgaatat gggtcgccgt
     4381 caggtatgga tgggcggtga agagttgatt ctgacgccca aagaatatgc tctgctgtca
     4441 cggttaatgc tcaaagcagg cagtccggtg catcgggaaa ttctctacaa cgacatctat
     4501 aactgggaca atgaaccctc gaccaacacc ctggaagtgc atatccacaa tctgcgcgac
     4561 aaagtgggca aagcccgtat ccgcaccgtg cgcggctttg gctatatgct ggtcgcgaat
     4621 gaggaaaact aattgaatct gatgcgtttt ctgcgccgac caatatcgct gcgccaacgg
     4681 ctgatattga ccattggggc cattttgttg gtgtttgagc tgatcagcgt cttctggcta
     4741 tggcatgaaa gtaccgagca gattcagctg tttgagcagg cgctacgcga caatcgcaac
     4801 aacgatagcc acatcatgcg tgagatccgc gaggcggtcg ccagcctgat cgtccccggc
     4861 gtctttatgg tcagcctgac gctatttatc tgctatcagg cggtacgccg catcacccgg
     4921 ccgctggcgg agctgcaaaa agagctggaa gcgcgcaccg ccgacaactt aacgcctatt
     4981 gccattcaca gcgccaccct cgaaatcgaa gcggtggttt cggcgttgaa cgatctggtc
     5041 agtcgcctga ccagcacgct ggataacgaa aggttgttta ccgctgacgt cgcgcacgaa
     5101 ctgcgaacgc cactggcggg ggtgcgtttg catctggaac tgctggcgaa aacgcatcac
     5161 attgatgtag caccgttagt ggctcgactt gatcagatga tggagagtgt ctcccagctg
     5221 ctgcaactgg cgcgtgccgg acagtcattt tcttccggta attatcaaca tgtaaaactg
     5281 ctggaagatg tcattctccc ctcgtatgac gaactcagta ccatgctcga ccagcgacag
     5341 caaaccctgc tattgccaga gagcgccgcc gacatcaccg ttcagggtga tgcaaccctg
     5401 ctgcggatgt tattgcgaaa cctggtagaa aacgcccatc gttacagccc gcaaggcagc
     5461 aacattatga ttaagctgca agaagatggc ggagcggtca tggccgttga agatgaagga
     5521 ccgggtattg atgagagtaa atgcggggag ttgagtaaag cgtttgtacg tatggacagc
     5581 cgttatggcg ggattggtct ggggttaagt attgtcagcc gcattacaca gttgcatcac
     5641 gggcagtttt tcctgcaaaa ccggcaagag acttccggca cgcgggcctg ggtacggctg
     5701 aagaaagatc agtacgtggc aaaccagata taaagaaagc tgctgaccac cagcacgctg
     5761 aacacggtag ttaaactttc ataaacacgg tttttcattg cactctcctc cgttaacctg
     5821 gaggagagta tgcgcgtcag agattaagtg aaccttaaga cttcaattgg cctgataaga
     5881 cagcgtcaca tcaggccatc cgtttcagct tattcatcaa ttcgcggatg ttgctgcacc
     5941 aggcgagtac gtttcgcctg caaatcggca atctcgtggt caatatcatc gattttctgc
     6001 tcgatattat cgtaatgctc gacgagaatt tccttcgctt cctgtatatc tgacgccgcc
     6061 ggtgtcgcac ctttcaacgg acgatttgcc gtctctttca tggttacgcc ggtgattaaa
     6121 ccaaccaccg ccactaccat caggtaatag gcaggcatca tcagattctg cgagctttcg
     6181 accagccagg ccgccagtgt tggcgtcaga ccggcaacca gcaccgaaat attaaatgcc
     6241 gccgccagcg cgctgtatcg gatatgcgtc gggaacatcg ctggcaaggt agaagccata
     6301 acgcccgtaa agcagttaag gatcaccgcc agcatcagta acccggcaaa aatcaggccg
     6361 atgacgttac tgttaatcag aataaacgcc gggatcgcca acacaaacag tgcaacacta
     6421 ccaagtagca caaacggacg acggccaaaa cggtcactca gcaagcccat caccggctgg
     6481 acaaacagca taccgatcat aatagcgata ataatcagca ccccgtggtc ttcggagtaa
     6541 tgcaggttat gcgacaaata actcggcata taggtcagca acatgtagta agtcacgttg
     6601 gtggcaatta ccagaccaat acatgtcaac aggctgcgcc agtgtttggt ggcaatctct
     6661 ttaaacgaga ctttcgggcc atcctgcaaa ccttcgcggt cgccctgttc cagtttatca
     6721 acatgctgct ggaacgccgg agtctcttcc agcgcatggc gcaggtaaag cccgataatc
     6781 cctaacggca gagcaataaa gaacggaata cgccagcccc agtcgaggaa gttcgcttcg
     6841 ccgacaatgg tcgaaattaa caccaccacg cccgcaccca gcacaaaccc ggcaatagaa
     6901 ccgaagtcca gccagctgcc cataaatcca cgtttacggt caggtgagta ttccgcaaca
     6961 aatatcgacg ccccggtata ttcaccacca accgagaaac cttgtgccat cttacagatc
     7021 aacagcaaaa tcggtgccca aataccaatc gtgtcgtagg acggtattaa gccaatacag
     7081 aacgtactga tcgacataat cacaatagtg atagcgagga tcttctggcg accatattta
     7141 tcgcccaaca taccaaagaa gagtccacca agcggtcgaa tcagaaaggg aacggagaaa
     7201 gtggcaagtg cagcaaccat ctgcacgctg gggtcagccc ccgggaaaaa aactttacct
     7261 aatgcgtaag caacaaaacc ataaacacca aaatcgaacc attccattgc attacccagt
     7321 gatgctgcgg taatggcttt acgcagttta ccgtcatcaa taatggtgac atcacgaagg
     7381 gtaatcggtt ttactttttt ccttttcagc atagctttcc tcgcagagtt ggcccatttc
     7441 cgcaaacgcc agaaaaatca gcgcgacggc cgcaaaaggg gagcgggaat tgcaggatgc
     7501 aatctctgta acaagcgtag cgggtttgct tacaccctcc ggttaaggaa tgtacaatca
     7561 aaccagttga cgcctgaatt gggcagttaa tgaccttttt accctaccag tgtttaaatt
     7621 tgtgatcaac ttcacacatg gatttcgttt aggactcatt gatgtaactg attatttaca
     7681 ccaaatttac agagaagatt tcctcgacca ttgcccccgc cagacgctgc gctgaacaga
     7741 gtcgtggcat ccccagcgcg acagtttgcc agcgttgggt cactgaccag caaacgggtt
     7801 gtttatctgc cgtggtccac tttcccagcc aacttaaaaa atcagcatga tccccgcccg
     7861 tcggaatggt cgaagaggca aaccactgat ggtaatagtg acgcgtggtc ggtgccgcat
     7921 tgatactctc tgccagatgt tgtgtcgctt tctcgcgcaa atcacccagc aacaacgcca
     7981 ccgcttgcgg gcttcccaga cggcaggcat cgccaaatgc cccccagcgc aactcctcca
     8041 gaacgataaa acagcgccca ggcaacgacc aggcgttata cgccccagca cgccagcggg
     8101 taaaaatttg ctcgctctga tcacctgcct gcaccagtag gcctcgttgc gttaacgcct
     8161 tttccttcca gcgacaacgg gctgaaaacc actcatacaa ggcggcaatt tgctggggga
     8221 gctccgggaa agcagagtgc tggcggtgat ccattttttg cagcagcgcc agatgttgcg
     8281 cgatctgcgt cagcgccaga tgacctggat ccagcatcga agccagtttt tccaccaatg
     8341 tcagacgcag gctaagcggc gcatttaaca tcccgccgtt cgtccatgcc tgaagctgca
     8401 cacgcgcaag aagttcctgc atcagtcgtt cacgaaactg ctgctgatgg gcttgtggat
     8461 ggcgggcatc atcgccctgc gccagatcca ctaaaaagcg cggatgaacc gactccagca
     8521 cgcgaccggg gccgtccagt aacgtcttgg tcaatatcgt tctgccgtga aaagtgtttg
     8581 aatatcatcg cgtaacagct gagcgtcggt gtaaatccag ctgtgagtca tcacagcctg
     8641 ctgcaattgc tgctgcatca gcctgaccac cgattcattt tgttgacgca gagccaggct
     8701 ttcgcgtaaa cgcgtctgta attccgtcaa acatgaagcg aactcagcga aaaaagtatt
     8761 catgcctgcc gtaacagatt catcgacctg ctctgccaga actttagcca tttgttggca
     8821 ataaagatcg acttctgcgc ttaatgctcg ttgcaacaca ctgtaatcaa ccgtctctgt
     8881 cggggatttc tcatttcccc gtccccagtc gggctgattc aaccagcgcg aaaaagtctc
     8941 acgcacaacg cctaaacgcg tgctctgctc gtccgttgct tcctggcgcg aaatgacttc
     9001 actgaacagc tggcgagtgt tgaagtgggg aactgcgccg tgaaaaacag gaaaatgaaa
     9061 cccaggacga aaccctgact cgctcaattc cattttgatt tgttgctcaa tggggcgaat
     9121 aacatcggtt aacacccggc aaagggtgga ttccagctcg gcaaaacgca gcgtaaagtc
     9181 gcgactaatg gtgttctgcg ccgtctgtaa cagtgtctca cagcgggtac gaatctcgct
     9241 taacggctcc gaatcatcct gaaacaaggc ggctaactgc gcattcagcg catcttgctg
     9301 ttgacgcaga aagtggttgg cggaggtcag ggccagctcg atttcatgtt taatctcgcc
     9361 gctcacctgc gcctgattga gttgcaatag ctgcaaactt tcttcgatct gatggatatt
     9421 ttgccgcaat tgttcacaag cgacgtttaa cccgtgcgca cgaaaatcca ggtattcccg
     9481 cgcctgctgc gcgtaattca acagtttatg cgcggcagat cgcagagcat acaacgaggc
     9541 gttagcgtaa gcggcatgaa gcaacgcctg aattggctgg gcgaacagcg aatcttccca
     9601 caactgatcg gcagcatgac gaatatgttc gaagtcagcc agatcggcat gacgccagcg
     9661 cctgccgagc gcggcatggg caaaatcttc cacccagcgt tgttgctctg gcggtggtaa
     9721 cttaccgttg ttggctaact catggcgcgc ccgattcgcc aggtagcccc acatcgacga
     9781 caccggaaat atctgctgtg gcgtaataca gcctttcatc agcgtcccgg aaatcagtgc
     9841 ccgcacctgg tcggcgtcgt cactgttacg atcctgttga tcgaacttat tgaccagcac
     9901 atacagcggc accgattgcc ccaccgccaa aatcgcctca cggacctctt catcggagat
     9961 cgatttcagt tgcgtataat ccagcaccgc cagtaccgcc gaggcgcgag ccagctgctg
    10021 gttaagcatt ttttgcagat gcggttgccc ggcttcattt ggcccggggg tatccagtaa
    10081 cgtcaactga ccgggataac tctccagccc cgccagatgg acaaactcca cttcaatcac
    10141 gggaatatgc tcaatggcgg cgtaagcaga aaaaggaaaa tcgacgtcca gcgccttcgc
    10201 cagtcgcacc aaatcattca aacttttcag acaatgaaaa ataggctggg cacccagata
    10261 atatttttcg aaagcgacgc cattttcgat ccgctgcata agcgcacgca tatctttatc
    10321 tatttccagc acatcggtca gatgcttaat atcgcaatca cgcaggcgct gttgtaattt
    10381 ttgaattaaa caatcgattg gcgcgacatg tgaaaaatgc agtaccggtt ccttttgccc
    10441 gggcgtatgg cgaataagcg tcggcagcgc agtcattggg cgattacgat taggcaaaac
    10501 ctccgtacca acaatggcat taatggtggt tgatttccct gctttcatgg taccgacaat
    10561 cgccagcacc atttccagtc gggaaatttt acgcaactca ttattcagca tcgcgtgacg
    10621 ttcggcgata ttaggctgac tccagggtaa agccagttgt ggcgcatcgt ctccggggac
    10681 agagagaggc attttttcca gtaactgcaa ctgttggcga gaaagctgta acaggcgttc
    10741 agcctcctga cttaactcat acagggtctg tgtgtacata gaaaattctt ccttaaagca
    10801 aattttgtta cttttattta gccagattgt ttttgagttc tgttttcggc ttttataatt
    10861 actgcaagaa ataattttat atttagagtg ttgtttttta ttag
//
LOCUS       DACBVI010000147        10787 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.148, whole genome shotgun sequence.
ACCESSION   DACBVI010000147 DACBVI010000000
VERSION     DACBVI010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.148"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            169..1122
                     /locus_tag="IU325_18540"
     CDS             169..1122
                     /locus_tag="IU325_18540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP5811003.1"
                     /translation="MAKKAGQIVGRITAQIDTLHRERYGKDTGHFGMIDAIDDPQVFA
                     ALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYAAHIEQ
                     LGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQILREIFN
                     SGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNINEAIAD
                     LNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEALRDPFA
                     RRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1129..1893)
                     /gene="glcC"
                     /locus_tag="IU325_18545"
     CDS             complement(1129..1893)
                     /gene="glcC"
                     /locus_tag="IU325_18545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HAP5811004.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAACDHVRTVKKNLHDIELEGHHLIRSAVPLEMKQG"
     gene            2144..3643
                     /gene="glcD"
                     /locus_tag="IU325_18550"
     CDS             2144..3643
                     /gene="glcD"
                     /locus_tag="IU325_18550"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HAP5811005.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLHVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARAQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3643..4695
                     /gene="glcE"
                     /locus_tag="IU325_18555"
     CDS             3643..4695
                     /gene="glcE"
                     /locus_tag="IU325_18555"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HAP5811006.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRATLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPCDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4706..5929
                     /gene="glcF"
                     /locus_tag="IU325_18560"
     CDS             4706..5929
                     /gene="glcF"
                     /locus_tag="IU325_18560"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HAP5811007.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPHPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVTPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            5934..6338
                     /locus_tag="IU325_18565"
     CDS             5934..6338
                     /locus_tag="IU325_18565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HAP5811008.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6360..8531
                     /gene="glcB"
                     /locus_tag="IU325_18570"
     CDS             6360..8531
                     /gene="glcB"
                     /locus_tag="IU325_18570"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HAP5811009.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPDRVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGNAAAPSCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQSEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8886..10568
                     /gene="glcA"
                     /locus_tag="IU325_18575"
     CDS             8886..10568
                     /gene="glcA"
                     /locus_tag="IU325_18575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HAP5811010.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKLDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
BASE COUNT         2507 a         2725 c         3035 g         2520 t
ORIGIN      
        1 attaaaattg aaaaagtaat taataaaaat gaccttaagg catttatcgc ttttccgtca
       61 tcactttatc ccgacgatcc aaactggata ccccctttat tcattgaacg caacgagcat
      121 ttgtctgcga aaaatccggg gacggaccat atcatctggc aggcgtgggt ggcaaaaaaa
      181 gcagggcaga tagttgggcg tattaccgcg caaatcgata ccttgcaccg cgagcgttac
      241 ggcaaagata ccggtcattt cggcatgatt gacgccattg atgatccgca ggtttttgcc
      301 gcgttgtttg gcgcagcgga agcgtggttg aagtcacaag gtgcaagtaa gatcagcggt
      361 cctttcagcc tgaatatcaa tcaggaaagc ggattactga ttgaaggttt cgatacacca
      421 ccctgtgcga tgatgccaca cggcaaaccg tggtatgccg cgcatattga acaattgggt
      481 tatcacaaag gcattgattt actggcgtgg tggatgcagc gaaccgatct cactttctct
      541 ccggccctaa aaaaactgat ggatcaggtg cgcaaaaaag tgaccattcg ctgcatcaat
      601 cgccagcggt ttgccgaaga gatgcaaatc ctgcgtgaga ttttcaactc tggctggcag
      661 cacaactggg gatttgtgcc gtttaccgaa catgagttcg cgaccatggg cgatcaactt
      721 aaatatctgg tgccggacga tatgatctac atcgctgaga ttgattctgc accctgcgcg
      781 tttattgtcg gcttgccgaa catcaacgag gcgattgccg atctgaacgg atcgctcttt
      841 cccttcggct gggcaaaatt gctgtggcgt ttgaaagtca gcggtgtgcg aactgcgcga
      901 gtaccgctga tgggcgtgcg ggacgagtat cagttcagcc gcatcggccc ggtgattgcg
      961 ctgttattga ttgaagcctt acgcgatccg tttgcccgcc ggaagatcga tgcgctggag
     1021 atgtcatgga tcctcgaaac caatacaggc atgaataaca tgctggaacg cattggggcg
     1081 gagccgtaca agcgttaccg attgtatgaa aagcagattt gatggcagtc acccctgctt
     1141 catctccagc ggcaccgccg agcgaatcaa atggtggcct tccagctcga tatcgtggag
     1201 attctttttc acggtccgca catgatcgca tgctgcgcgc tgggcgacgt gcggcagccg
     1261 ctgcaacacc gcgttgtaga tccgcgcatg ctggcgatcg atctgctttt tttgctgtgg
     1321 tcgatggtag agattattta cgctggcaaa cactgaatta aacatcagat cggtcaatga
     1381 ttgcagcgta aacaccaaca cctgattgtg agaagcctga caaatggcga gatggaaagc
     1441 gtgatccaac tgcgcatgtt cgatcagcga aatctctttg ttgttctcac tggcggcgag
     1501 cattttttca taacagcggg ttatcacaac aaaatcagcc tgggttccca gcgttgccgc
     1561 aagccttgcc gattcaccct ccagcaatgc gcgaacgtcg agcagatcgt acagcgttcg
     1621 cggctgcgtg ctgaacagat gaattagcgg gctggtatcc tgcacccgat taagccgtgc
     1681 gacacgagaa tcgcgaccct gcgccgtttc aataatcccg cgcccgcgca gcacggtcag
     1741 cccttcacgc agtgcggagc gtgagaagcc gagcttttca cacagtcgac gttccgaggg
     1801 aagcggctga ccgaccttca gtacgccgtc gataattaac cgttcgatac tctctgcaac
     1861 cacttcgcaa atagggcgac gttcatcttt cattcccgga cctcgtgcac aggtaggacc
     1921 aatttttctg ttcttgactc gcaaaacacg cacatcacgt aagtgattat gttacatcaa
     1981 tttaacatta agttaaccaa gacaaggtca cagagctgga aaaaaaatgg tctgaccggt
     2041 agctaaagag atagacgaaa acgaaaagcc cgcttaataa ctgttcacag aagcagcgcg
     2101 caaaaatcag ctgccacaca acacaacaaa gcgaagccta ctcatgagca tcttgtacga
     2161 agagcgtctt gatggcgctt tacccgatgt cgaccgcaca tcggtactga tggcactgcg
     2221 tgagcatgtc cctggactgg agatcctgca taccgatgag gagatcattc cttacgagtg
     2281 tgacgggttg agcgcgtatc gcacgcgtcc attactggtt gttctgccta agcaaatgga
     2341 acaggtgaca gcgattctgg ctgtttgtca ccgcctgcat gtaccggtgg tgacccgtgg
     2401 tgcaggcacc gggctttctg gtggcgcgct gccgctggaa aaaggtgtgt tgttggtgat
     2461 ggcgcgcttt aaagagatcc tcgacattaa ccccgttggt cgccgcgcgc gcgcgcagcc
     2521 gggcgtgcgt aacctggcga tctcccaggc cgttgcgccg cataatctct attacgcgcc
     2581 ggatccttcc tcacaaatcg cctgttcaat tggcggcaat gtggcggaaa atgccggtgg
     2641 cgtccactgc ctgaaatatg gtctgaccgt acataacctg ctgaaaattg aagtgcaaac
     2701 gctggacggc gaggcgctga cgctgggatc ggacgcgctg gattcgcctg gttttgacct
     2761 gctggcgctg ttcaccggat cggaaggtat gctcggcgtg accaccgaag tgacggtaaa
     2821 actgctgccg aagccgcccg tggcgcgggt tctgttagcc agctttgact cggtagaaaa
     2881 agccggactt gcggttggtg acatcatcgc caatggcatt atccccggcg ggctggagat
     2941 gatggataac ctgtcgatcc gcgcggcgga agattttatt catgccggtt atccggtcga
     3001 cgccgaagcg attttgttat gcgagctgga cggcgtggag tctgacgtac aggaagactg
     3061 cgagcgggtt aacgacatct tgttgaaagc gggcgcgact gacgtccgtc tggcacagga
     3121 cgaagcagag cgcgtacgtt tctgggccgg tcgcaaaaat gcgttccctg cggtaggacg
     3181 aatctccccg gattactact gcatggatgg caccatcccg cgccgcgccc tgcctggcgt
     3241 actggaaggc attgcccgtt tatcgcagca atatgattta cgcgttgcca acgtctttca
     3301 tgccggagac ggcaacatgc acccgttaat ccttttcgat gccaacgaac ccggtgaatt
     3361 tgcccgcgcg gaagagctgg gcgggaagat cctcgaactc tgcgttgaag ttggcggcag
     3421 catcagtggg gaacatggca tcgggcgcga aaaaatcaat caaatgtgcg cccagttcaa
     3481 cagcgatgaa atcacgacct tccatgcggt caaggcggcg tttgaccccg atggtttgct
     3541 gaaccctggg aaaaacattc ccacgctaca ccgctgtgct gaatttggtg ccatgcatgt
     3601 gcatcacggt catttacctt tccctgaact ggagcgtttc tgatgctacg cgagtgtgat
     3661 tacagccagg cgctgctgga gcaggtgaat caggcgatta gcgataaaac gccgctggtg
     3721 attcagggca gcaatagcaa agccttttta ggtcgtcctg tcaccgggca aacgctggat
     3781 gtgcgttgtc atcgcggcat tgttaattac gacccgaccg agctggtgat aaccgctcgt
     3841 gccggaacgc cgctggtggc gattgaagcg gcgctggaga gcgccgggca aatgctcccc
     3901 tgcgagccgc cgcattatgg tgaagaagcc acctggggcg ggatggtcgc ctgcgggctg
     3961 gcggggccgc gtcgcccgtg gagcggttcg gtccgcgatt ttgtcctcgg cacgcgcatc
     4021 attaccggca ctggaaaaca tctgcgtttt ggtggcgaag tgatgaaaaa cgttgccgga
     4081 tacgatctct cacggttaat ggccggaagc tacggttgtc tgggtgtgct cactgaaatc
     4141 tcgatgaaag tgttaccgcg accacgcgcc accctgagtc tgcgtcggga aatcagcctg
     4201 caagaagcca tgaatgaaat cgcccagtgg caactccagc cattacccat tagtggctta
     4261 tgttacttcg acaatgcgtt gtggatccgc cttgagggcg gcgaaggatc ggtaaaagca
     4321 gcgcgtgaac tgctgggggg cgaagaggtt gcgggtcagt tctggcagca attgcgtgaa
     4381 caacaactgc cgttcttctc gttaccaggt accttatggc gcatttcctt accctgcgat
     4441 gcgccgatga tggatttacc cggcgagcaa ctgatcgact ggggcggggc gttacgctgg
     4501 ctgaaatcga cagccgagga caatcaaatc catcgcatcg cccgcaacgc tggcggtcat
     4561 gcgacccgct ttagtgccgg agatggtggc tttgccccgc tatcggctcc tttattccgc
     4621 tatcaccagc agcttaaaca gcagctcgac ccttgcggcg tgtttaatcc cggtcgcatg
     4681 tacgcggagc tttaaggagc aggctatgca aacccaatta accgaagaga tgcggcagaa
     4741 cgcgcgggcg ctggaagccg acagcatcct gcgcgcctgt gttcactgcg gattttgtac
     4801 cgcaacctgc ccaacctatc agctcctggg cgatgaactg gacgggccgc gcgggcgcat
     4861 ctatctgatt aaacaggtgc tggaaggtaa cgaagtcacg cttaaaacac aggagcatct
     4921 cgatcgctgt ctcacttgcc gcaattgtga aaccacctgt ccttctggtg tgcgctatca
     4981 caatttgctg gatatcgggc gtgatattgt cgagcagaaa gtgaaacgcc cactgccgga
     5041 gcgaatgctg cgcgaaggat tgcgccaggt agtgccgcat ccggctgtct tccgtgcgct
     5101 gacgcaggta gggctggtgc tgcgaccgtt tttaccggaa caggtcagag caaaactgcc
     5161 tgctgaaacc gtaaaagcta aaccgcgtcc accgctgcgc cataagcgtc gggttttaat
     5221 gctggaaggc tgcgcccagc ctacgctttc gcccaacacc aacgcggcaa ctgcgcgagt
     5281 gctggatcgt ctggggatca gcgtgacgcc agctaatgaa gcaggctgtt gtggcgcggt
     5341 ggactatcat cttaatgcgc aagagaaagg gctggcgcgg gcgcgcaata atattgatgc
     5401 ctggtggccc gcgattgaag caggtgccga ggctattttg cagactgcca gcggctgcgg
     5461 cgcgtttgtc aaagagtatg ggcagatgct gaaaaacgat gcgttgtatg ccgataaagc
     5521 gcgtcaggtc agtgagctgg cggtcgattt agtcgaactt ctgcgcgagg aaccgctgga
     5581 aaaactggca attcgcggcg ataaaaaact ggccttccac tgcccgtgta ccctgcaaca
     5641 tgcgcaaaag ctgaacggcg aagtggaaaa agtgttactt cgtcttggat ttaccttaac
     5701 ggacgttccc gacagccatc tgtgctgcgg ttcagcggga acatatgcgt taacgcatcc
     5761 cgatctggcg cgccagctgc gggataacaa aatgaatgcg ctggaaagcg gcaaaccgga
     5821 aatgatcgtc accgccaaca ttggttgcca gacgcatctg gcgagcgccg gtcgtacctc
     5881 tgtgcgtcac tggattgaaa ttgtagaaca agcccttgaa aaggaataac aaaatgaaaa
     5941 ctaaagtcat tcttagccag caaatggcga gtgcaattat tgccgcaggt caggaagagg
     6001 cgcagaaaaa taactggtct gtttccattg ctgttgccga tgacggcggt catctgctgg
     6061 cgttaagtcg catggacgat tgcgcgccga ttgcggctta catctcccag gagaaagcgc
     6121 gtaccgccgc gctggggcgt cgtgaaacta agggctatga agagatggtg aacaacggac
     6181 gtaccgcgtt cgtgactgcg ccgttattaa cgtcgctgga aggcggcgta ccggttgttg
     6241 ttgatgggca aattattggt gccgtgggcg tttctggttt aaccggagca caggatgcgc
     6301 aggtcgcgaa agcggcagca gcggtgttgg cgaaataagc gaaaacgagg agagaaacaa
     6361 tgagtcaaac cataacccag ggccgtttac gcattgacgc caattttaaa cgttttgtgg
     6421 atgaagaagt tttgccggga acagggttgg acgctgcggc gttctggcgc aatgttgatg
     6481 agatcgttca cgatctggca ccagaaaatc gtcagttgct ggcagaacgc gatcgcattc
     6541 aggcggcgct tgatgagtgg catcgcagca atccggggcc ggttaaagat aaagcggcct
     6601 ataaatcttt cctgcgtgaa ctgggctacc tggtgccaca accggatcgc gtgacggtgg
     6661 aaaccacggg cattgacagc gaaatcacca gccaggcggg gccacagctg gtggttccgg
     6721 caatgaacgc ccgctacgcg ctgaacgcgg cgaacgctcg ctggggctca ctgtacgatg
     6781 cgttatacgg cagcgacatc atcccgcagg aaggggcgat ggtcagcggc tacgatccgc
     6841 agcgcggtga gcaggttatc gcctgggttc ggcgtttcct cgatgaatca ctaccgctgg
     6901 aaaacggcag ctatcaggat gtggtggcgt ttaaggtggt tgataaacaa ttacgcatcc
     6961 agttgaaaaa tggtaaagaa accacgttac gcaccccggc gcagtttgtc ggttaccgtg
     7021 gcaatgccgc tgcgccgtcc tgcattttgc tgaaaaataa cggcctgcat attgagctgc
     7081 aaatcgatgc caacgggcgg attggcaaag acgatccggc gcatatcaac gatgttatcg
     7141 tcgaagcggc catcagtacc attctcgact gcgaagattc ggtcgcggcg gttgatgcgg
     7201 aagataaaat cctgctgtac cgcaacctgc tgggcctgat gcaggggact ctgcaagaga
     7261 aaatggaaaa aaacggtcgg caaatcgtgc gtaaactgaa tgacgatcgt cattacaccg
     7321 ccgccgatgg ctctgaaatt tctctgcacg gacgctcgct gctgtttatc cgcaacgtgg
     7381 gtcatttgat gaccattcct gtgatttggg acagcgaagg caatgaaatc ccggaaggca
     7441 ttcttgatgg cgtcatgact ggcgcgattg ccctctatga tttaaaagtg cagaaaaact
     7501 cgcgcactgg cagcgtctat attgtgaaac cgaaaatgca cggtccgcag gaagtggcgt
     7561 tcgccaacaa actgtttacc cgcattgaga caatgctcgg tatggcaccg aataccctga
     7621 aaatgggcat tatggatgaa gaacgtcgga cctcgctgaa cttgcgtagc tgtatcgctc
     7681 aggcgcgcaa ccgcgtggcg ttcatcaata ccggtttcct cgaccgtacc ggcgatgaaa
     7741 tgcattcggt gatggaagct ggcccgatgc tgcgtaaaaa tcagatgaaa tcgacgcctt
     7801 ggatcaaagc ctacgagcgt aataacgtgc tttccggtct gttctgtggg ctgcgcggta
     7861 aagcgcaaat tggtaaaggc atgtgggcaa tgccggacct gatggcagac atgtacagcc
     7921 agaagggcga ccaactgcgt gccggggcaa acacagcctg ggttccgtca ccaaccgctg
     7981 ctacgctcca tgcgctgcac taccaccaaa ccaacgtaca gagcgtgcag gccaacattg
     8041 cccagagcga gttcaatgct gaatttgaac cgctgctgga cgatctgctg actattccgg
     8101 tggctgaaaa cgctaactgg tcggtggaag agatccaaca agagctggat aacaacgtgc
     8161 aggggattct ggggtacgtg gtgcgttggg tggagcaggg gattggttgt tcaaaagtgc
     8221 cggatattca caacgtggcg ctgatggaag accgcgcaac gctgcgtatc tccagccagc
     8281 atatcgccaa ctggttacgt cacggtattc tgaccaaaga acaggtgcag gcgtcgctgg
     8341 agaatatggc gaaagtggtt gatcagcaaa acgctggcga tccggcttat cgtccgatgg
     8401 cggggaattt cgctaactcg tgtgctttta aagctgccag cgatttaatc ttcctcggcg
     8461 tgaaacagcc aaacggctat accgaaccgt tattacacgc ctggcgttta cgcgaaaaag
     8521 aaagtcatta atagcgtcaa aaaagccccg gcaagtttta ttgtcggggc tatgatagta
     8581 actgcggtag tacatttatc ccggaaataa ccggagcgat tatcagggtt taacaatatt
     8641 aaatacccgt agcgcattac tagcgggcct aaaacaacaa tatggacatc ttaacgatac
     8701 tctgcgcctg cgtgtgcgga acagaaaccc ttacccgata tacttgttat gccagaggta
     8761 gcaattgcgt tagcagcgta ctgttgctgt aaagtataac gcctatattt tacagttcag
     8821 tcctcttttg cttattacaa ccttaagtat atcaagcata taaagataat aagagactga
     8881 acaatatggt tacctggacc caaatgtata tgccgatggg aggactgggg ctatccgctc
     8941 tggtcgccct gatcccgata atattcttct tcgttgcact cgcggtatta cgtctgaaag
     9001 gacatgtcgc tggagcaata acccttatat tatctatcct gattgcaata ttcgccttta
     9061 aaatgccgat tgatatggca tttgctgctg cgggctatgg ctttatttat ggtttatggc
     9121 ctattgcgtg gattattgtc gcggcggtat tcttgtataa attaaccgtt gccagcggcc
     9181 agttcgatat tatccgcagc tcggttatct ccatcaccga cgatcagcgt ttgcaggtgt
     9241 tactgattgg tttctccttt ggcgcgttgc tggaaggggc ggctggcttt ggtgcgccgg
     9301 tggcgattac cggtgcgctg ctggtgggcc tgggcttcaa accgttatac gcggcggggc
     9361 tgtgtctgat tgccaacact gcgccggtgg cgtttggtgc gttgggcgtg ccgattctgg
     9421 tcgccggtca ggtaacggga atcgatccgt tccacattgg cgcaatggcg ggacgtcagt
     9481 taccgttcct gtcggttctt gtgccgttct ggctggtagc aatgatggac ggctggaaag
     9541 gggtgaaaga gacgtggcca gcggcgctgg ttgctggggg aagcttcgct gtcactcagt
     9601 tctttacctc taactatatt ggtccggaac tgccggatat tacttcggcg ctggtgagta
     9661 tcgtctcact cgctttattc cttaaagtct ggcggccgaa aaataccgaa acggcaatca
     9721 gcatgggaca atccgcaggt gcgatggtgg tgaataaacc atcttctggc ggtcccgtgc
     9781 cttcagaata tagtctgggg caaatcattc gtgcgtggtc accgttttta atcttaacgg
     9841 tgctggtcac catctggacc atgaagccgt ttaaagcgtt atttgctccg ggcggcgcgt
     9901 tttattcact ggtgattaat ttccagatcc ctcatttgca tcaacaagtg ttgaaagcgg
     9961 cacccattgt cgcccaacca acgccaatgg atgcggtgtt taaattagac cctctctcgg
    10021 ctggcggcac cgctattttt attgcggcga ttatctctat cttcatcctc ggcgtgggca
    10081 tcaagaaagg gattggcgtc tttgccgaaa cgctaattag tctgaagtgg ccgatattgt
    10141 cgattggcat ggtgctggcg ttcgccttcg tcaccaacta ttccggtatg tccaccacgc
    10201 tggcgctggt actggcaggt acaggcgtga tgttcccgtt tttctcaccg tttctcggct
    10261 ggctgggcgt attccttacc ggctcggaca cctcctctaa cgccctgttt ggttcactgc
    10321 aatcgaccac ggcgcagcaa atcaacgtct ctgacactct gctggtggca gcaaatacca
    10381 gcggcggcgt aactggcaag atgatctccc cgcaatctat cgccgtggcc tgcgccgcga
    10441 cgggcatggt gggccgagaa tctgaactgt tccgctacac cgtgaagcac agtctgattt
    10501 ttgccagcgt tatcggcatt atcacgctgt tgcaggcgta tgtgtttacc gggatgttag
    10561 tttcgtaagt caataatgcc ggatgatcgt taatatcatc cggctacatt tacattattc
    10621 tgtcttatta attgaaaatt atttccctct ttattatcca tgcagcaata atgttttgcg
    10681 ttcagataac ctgctaaata attaagaacg tgagaatatc tattttttta tcacgcaaaa
    10741 caagtttgta ctaattatgt tcgcttgatg agcaaacacc gtgctat
//
LOCUS       DACBVI010000148        10680 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.149, whole genome shotgun sequence.
ACCESSION   DACBVI010000148 DACBVI010000000
VERSION     DACBVI010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.149"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            149..2290
                     /locus_tag="IU325_18580"
     CDS             149..2290
                     /locus_tag="IU325_18580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HAP5811011.1"
                     /translation="MAKFTPSFSGIKGRALFSLLFAAPMIHATDTATTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFSDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYGDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHSTRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KKAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESIGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(2354..4246)
                     /gene="parE"
                     /locus_tag="IU325_18585"
     CDS             complement(2354..4246)
                     /gene="parE"
                     /locus_tag="IU325_18585"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP5811012.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(4275..4856)
                     /gene="yqiA"
                     /locus_tag="IU325_18590"
     CDS             complement(4275..4856)
                     /gene="yqiA"
                     /locus_tag="IU325_18590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HAP5811013.1"
                     /translation="MSMLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(4856..5683)
                     /gene="cpdA"
                     /locus_tag="IU325_18595"
     CDS             complement(4856..5683)
                     /gene="cpdA"
                     /locus_tag="IU325_18595"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HAP5811014.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(5708..6130)
                     /locus_tag="IU325_18600"
     CDS             complement(5708..6130)
                     /locus_tag="IU325_18600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HAP5811015.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(6131..6760)
                     /locus_tag="IU325_18605"
     CDS             complement(6131..6760)
                     /locus_tag="IU325_18605"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HAP5811016.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            6965..8446
                     /gene="tolC"
                     /locus_tag="IU325_18610"
     CDS             6965..8446
                     /gene="tolC"
                     /locus_tag="IU325_18610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HAP5811017.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            8594..9265
                     /locus_tag="IU325_18615"
     CDS             8594..9265
                     /locus_tag="IU325_18615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HAP5811018.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            9271..10431
                     /locus_tag="IU325_18620"
     CDS             9271..10431
                     /locus_tag="IU325_18620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAP5811019.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
BASE COUNT         2719 a         2827 c         2598 g         2536 t
ORIGIN      
        1 atttctcatt tactactaca tcaccgcatg gttgaatgat tctcaattac atatccgatg
       61 gtgaaataac aaacggatag tgcttagtga gcaacaagaa acaattttca ctcacgacaa
      121 tccgtgagtt tttgggataa cagactaaat ggctaagttc acaccttcat tctcaggaat
      181 caaaggtcgg gcgctctttt cactgctctt tgcagcacca atgattcatg caaccgacac
      241 tgcaacgacc aaagatggcg aaacaatcac tgttacagcg gatgcaaata ccgcaactga
      301 ggcgaccgat ggttatcaac ctctgagcac ttccacggcg acattaaccg atatgccgat
      361 gctggatatc ccgcaggtgg tcaatacggt tagcgatcag gttctggaaa atcagaatgc
      421 gacgacgctg gatgaagcgc tttataacgt cagtaacgtg gtacagacca atacattagg
      481 cggaactcag gacgcctttg tacgtcgtgg gtttggtgct aaccgggatg gctccatcat
      541 gaccaacggc ctgcgaactg tacttcctcg cagtttcaac gccgccacag aacgtgtgga
      601 agtgctaaaa ggtccggcct ccacgctgta tggcattctc gatcctggtg gattgattaa
      661 cgtcgtgacc aagcgcccgg aaaaaacatt ccatggttct gtctcagcca cgtcctccag
      721 ttttggcgga ggcactgggc aacttgatat cacaggtccc attgaaggca ctcagctggc
      781 ataccgcctg acgggggaag tgcaggatga agattactgg cgaaacttcg gtaaagagcg
      841 cagtacattt attgccccgt cactcacctg gtttagtgat aatgcaacag taaccatgct
      901 ctattcccat cgggactata aaactccgtt cgatcgtgga acgattttcg accttacgac
      961 gaaacagccc gtaaacgttg atcgaaaaat acgttttgac gaaccgttta atattacaga
     1021 tggtcagtcc gatctggcgc aactcaacgc agaatatcat ctcaatagcc agtggacagc
     1081 gcgctttgat tacagctaca gccaggataa atacggcgat aatcaggctc gtgttaccgc
     1141 gtatgatgca acgacaggaa cgctgacacg gcgtgttgat gcaactcagg gatctaccca
     1201 gcgtatgcat tctactcgtg cggatctgca agggaatgtt gatattgctg ggttctataa
     1261 tgagattctg ggtggggtgt catatgaata ttatgatctt ctgcgcacag atatgattcg
     1321 ctgtaaaaaa gctaaagatt tcaatatcta caaccctgtt tatggtaata ccagcaaatg
     1381 tacaacggtt tcggcgtcgg acagtgatca gacgatcaaa caggagagct actcagcgta
     1441 tgcacaggac gcgctctatc tgaccgataa ctggattgcc gtcgccggga tccgctatca
     1501 gtattacacg cagtatgcgg gtaaaggccg tccttttaat gtcaatactg acagccgcga
     1561 tgaacaatgg actcccaaac tggggttggt ctacaaactg acgccatcgg tatccttatt
     1621 tgctaattat tcgcaaacat ttatgccgca atcgtcaatt gccagctaca ttggcgatct
     1681 tccaccggaa tcatctaatg cttacgaagt cggggcaaaa ttcgagctgt tcgatggtat
     1741 aaccgcagat attgcgctgt ttgatatcca taaacgtaac gtgttgtata ccgaaagtat
     1801 tggtgatgaa accatcgcca aaacggcagg ccgcgttcgt tcaagagggg tagaagtcga
     1861 ccttgcggga gcattaactg aaaacattaa tatcattgcc agctacggct ataccgatgc
     1921 taaggttctg gaagatcctg attatgcagg gaaaccattg ccgaatgttc ctcgtcatac
     1981 cggttcgcta ttcctgacct atgacattca taacatgcca ggcaataaca cactgacgtt
     2041 tggcggtggt ggacatggcg taagccgtcg ttcggcaacc aatggggctg actattatct
     2101 gccaggctat ttcgttgccg atgccttcgc cgcatacaaa atgaaattgc agtatccggt
     2161 cacactgcaa ttaaacgtca aaaacctgtt tgataaaacg tattacacct cttccatcgc
     2221 cacaaataat ctggggaatc agattggcga tccgcgtgaa gtgcaattca cggtgaaaat
     2281 ggaattttga tcaaaaaagc ccggtataca cttgtatacc gggctatgtt ttattcttct
     2341 ggcgggagga ttattagacc tcaatctccg ccatgtcgcc tttctcttgc aaccagttgc
     2401 ggcgatcttc cgagcgtttc ttcgccagca gcatatccat catcgcgtca gtacgctgat
     2461 cgtcttcatc atcgatagtc aactgcacca gacggcgagt gttcggatca agcgtggttt
     2521 cacgcaattg catcgggttc atttccccca gacctttaaa acgctggacg ttcggcttgc
     2581 ctttcttgcg ttttaattgc tcaagtacgc cctctttctc ttcttccgtc agcgcgtaat
     2641 aaacctcttt cccgagatca atacggtaga gcggtggcag tgcgacgtaa acgtgaccgt
     2701 gtttcaccaa cgcgcggaag tgttttacga acaaagcgca gagcagcgtg gcaatgtgca
     2761 gaccatcaga gtccgcatcc gcgaggatac agattttgcc gtaacgaagc tggctcagat
     2821 cgtcgctgtc aggatcgata ccgatcgcta ccgaaatatc gtgcacttcc tgcgaagcca
     2881 gcacttcgtc ggaggagact tcccaggtgt taaggatctt acctttcagt ggcatgatag
     2941 cctgatattc gcgatcgcgc gcctgcttgg cagatccgcc tgcggagtca ccttccacaa
     3001 ggaacagctc ggtacggtta aggtcctgcg cggtacaatc agccagtttg ccaggcaacg
     3061 ccgggccgct ggttagcttt ttacgcacca cttttttagc cgcacgcata cgacgctggg
     3121 cgctggaaat cgccatctct gccaacagct ccgccgcctg aacgttctgg ttcagccaca
     3181 ggatgaaggc atctttcacc acgccagaaa cgaatgccgc acactgacgc gaagagagac
     3241 gttctttggt ctgcccggca aactgcggat cctgcatttt tactgacagc acataggcgc
     3301 agcgatccca gatatcttcc gccgacagct tcacgccgcg cggcagaatg ttgcgatatt
     3361 cacagaactc gcgcatcgca tccagcaggc cctgacgcag gccattaacg tgcgtaccac
     3421 cctgcatcgt tgggatcagg ttgacgtagc tttcggtcag cagttcaccg ccttccggca
     3481 gccacagtag cgcccagtcc accgcttcag tatcgccagc gaaattaccg ataaacggtt
     3541 tttctggcag cgtcggtaaa ccgtttaccg cttccgccag gtaatcattc agaccgtcct
     3601 gatagcacca gcgctgttcg gtgttgttga tctcatcttt aaaggtgatc tcaacaccag
     3661 ggcacagtac cgctttggct ttcagcacat gtgtcaggcg tgaaacagaa aaacgcgggc
     3721 tgtcaaagaa ggtttcatcc ggccagaagt gcacgctggt accggtattg cgcttaccgc
     3781 aagtgccgac aacctgtaaa tcctgcacct tttcgccatt ttcaaaggcg atgttataaa
     3841 cctgaccatc gcggcgtacg ttaacttcta cgcgcttcga cagggcgtta actaccgaaa
     3901 tccccacgcc atgcaggccg ccagagaact ggtagttttt gttagagaat ttaccgcctg
     3961 catgcagacg gcaaagaatc agttcaaccg ccggtacacc ctcttccggg tgaatatcca
     4021 ccggcatccc gcgcccatcg tcaatcactt ctaacgactg gtcagcatgt aagattacat
     4081 ccacgcgttt tgcgtgaccc gccagtgctt catcgacact gttatcaata acttcttgcc
     4141 ccaaatggtt agggcgagtg gtatcggtat acatccccgg acggcggcga accggctcaa
     4201 gcccggtgag tacctcaatg gcatcagcgt tataagtttg cgtcatggtt taagttagca
     4261 attcgagttg atcgtcagag atggtgcaga ccaagaaaat cgacgatcgg gttgaaataa
     4321 tcttcgaagc ccgtgaatgc gtggttgccg ccttctatga cagtctggcg gcaggaagcg
     4381 tagtacgcca ccgcctggcg gtaatccagc acttcatctc ccgtctgttg cagcagccag
     4441 atcaaatccg gcgcttccag cgggtcaatc tgcatgactt tgagatcgta aatatggcgt
     4501 gactctagca catattgctg cccggtgtag gggttctcgt tctgaccgag atagtccgtc
     4561 agcagttcaa acgggcgcac cgccgggttt accaccactg cgggcagcat aaaacattgc
     4621 gacaaccagg tggcgtaata tccccccagt gacgaaccga caatacccag cgaatcgccg
     4681 ccatgttcca ggacaatgga ttccagcagt tctgccgcgt cggaaggata cggcggcaac
     4741 tgcggaatga tcatctcaac gtcagggtga tgttccgcca gccagttttt taacaagctc
     4801 gcttttgcag agcgcggcga gctgttgaaa ccgtgtaaat aaagaagcat agacatcagt
     4861 agccttctga agcggtatca ggttggaaac gtgtgtccgc caggcgatgc acctcggtgg
     4921 tcagcgtgcc atcagcatgt aactcgagag tacgccagcc gggcgcgatg gtatccagcg
     4981 taaagttgga acagtgcggc ttaaactgca cacaggtcga tggcgttgcc aacaggcggc
     5041 gaccgttcca gtcgagatcc agctcctgat gaatatgacc gcacagcaag tatttgacgt
     5101 gcggaaactt cgccagcacg gtatccagtt cgcccgcgtt acgcaggctg tgttggtcaa
     5161 gccaactaca acccgcaggt agcggatgat gatgcagcag cagcaacgta tggcgttctg
     5221 gcgcatcggc cagtttacgt tccagccact caagctgaaa ctcgctcagc tcaccgtgcg
     5281 gcacgccaaa cacctggcta tccagcaaca ggatttgcca ttgctcacca ataaacaccc
     5341 gcttcgccgg ggagataccc gcatcctgta acgcgctgta catcgcgggc tggaaatcgt
     5401 ggttgcccgg cagccagacg cagggcgcac gaaaacttgc aatgccttca gcgaaatgct
     5461 gataggccgc agaggattga tcctgcgcta aatcacctgt cgcgacaatc aggtcgaatt
     5521 cgtgctggtg tggccgaatc gcctccagca ccgcctggta gctttcccag gtgtttaccc
     5581 ccaacagggc ttcgtgcttt tgcgcaaaca ggtgagtgtc ggtaatttgt aaaatcctga
     5641 ctctggcctc accagccaga ggaagggtta acaggctttc caaatggtgt ccttaggttt
     5701 cacgacgcta ataaaccgga atcgccatcg ctccatgtgc taaacagtat cgcaaccagt
     5761 ccgctaaaaa ctgattaatt tgatgctttt cgtcgcgttg atgcaacttt ttattaggat
     5821 aatcataccg cgctttgaag cgaaaaatct gctggcttga acacacttca gccaccatcg
     5881 cgtcatgata cagacgcacc gtcattgacg gaaggctcca gtaactgatc gcgggcgcag
     5941 tctgttctat tgtcaccagg gtagtgtatc gggtcgattc cacaatcgtc agccgatatt
     6001 gtgcgtttgc cacctgatag cttacagttt cgccgggtgc gtcattgcgc ggtaacaaac
     6061 ggcgcaattg tgaaaagttc atctcgcaca ggcgcatcat ttcaggaaag tcaggtgtgt
     6121 aacgcttcat ttatgcccac tcatttttta acgcttgatg atgcagctgc agccattgca
     6181 aagcgatgac cgacgctgcg ttgtcgattt tcccctcttc tacccactgg tatgcctgtt
     6241 cccggcttac cacatgaacg cgaatatctt cgttttcatc agccagaccg tgaataccgc
     6301 ttgcggtcgt ggcgtccact tcgcccacca taattgacga acgctcactg gtgccccccg
     6361 ggcttgccag gaaacttaac accggtttgg tccgtttgac tatcagtccc gcctcttcaa
     6421 tcgcttcgcg acgggcaaca tcttccacac tttcaccctc ttcaatcatc ccggcaacca
     6481 tctccagtag ccagggggtt tcgctggtgt cgtacgcggc aatccgaatc tgctcaatca
     6541 gcacaacttc atcacgcact gggtcaaagg gtagcaagac tgcggcgtga ccgcgctcaa
     6601 aaatttcccg ccgtacctca tgactcattt gcccgttgaa tagacgatga cgaaatctat
     6661 aaagatctaa tgaaaaaaag ccgcgataaa gtgtttctcg tgcaataatt tctacatcgt
     6721 ttttgccaaa tgtaacgggc aggttgtctg gcttaagcat tgttaatgtc ctggcactaa
     6781 tagtgaatta aatgtgaatt tcagcgacgt ttgactgccg tttgagcagt catgtgttaa
     6841 attgaggcac attaacgccc tatggcacgt aacgccaacc ttttgcggta gcggcttctg
     6901 ctagaatccg caataatttt acagtttgat cgcgctaaat actgcttcac cacaaggaat
     6961 gcaaatgaag aaattgctcc ccattcttat cggcctgagc ctctctgggt tcagttcgtt
     7021 gagccaggcc gagaacctga tgcaagttta tcagcaagca cgccttagta acccggaatt
     7081 gcgtaagtct gccgccgatc gtgatgctgc ctttgaaaaa attaatgaag cgcgcagtcc
     7141 attactgcca cagctaggtt taggtgcaga ttacacctat agcaacggct accgcgacgc
     7201 gaacggcatc aactctaacg cgaccagtgc gtccctgcag ttaactcaat ccatttttga
     7261 tatgtcgaaa tggcgtgcgt taacgctgca ggaaaaagca gctgggattc aggacgtcac
     7321 gtatcagacc gatcaacaaa ccttgatcct caacaccgcg accgcttatt tcaacgtgtt
     7381 gaatgctatt gacgttcttt cctatacaca ggcgcaaaaa gaagcgatct accgtcaatt
     7441 agatcaaacc acccaacgtt ttaacgtggg cctggtagcg atcactgacg tgcagaacgc
     7501 ccgcgcacag tacgataccg tgctggcgaa cgaagtgacc gcacgtaata accttgataa
     7561 cgcggtagag cagctgcgcc agatcaccgg taactactat ccggaactgg cggcgctgaa
     7621 tgtcgaaaac tttaaaaccg acaaaccaca gccggttaac gcgctgctga aagaagccga
     7681 aaaacgcaac ctgtcgctgt tacaggcacg cttgagccag gacctggcgc gcgagcaaat
     7741 tcgccaggcg caggatggtc acttaccgac tctggattta acggcttcta ccgggatttc
     7801 tgacacctca tatagcggtt cgaaaacccg aggtgccgct ggtacccagt atgacgatag
     7861 caatatgggc cagaacaaag ttggcctgag cttctcgctg ccgatttatc agggcggaat
     7921 ggttaactcg caggtgaaac aggcccagta caactttgtt ggtgccagtg agcaactgga
     7981 aagcgcccat cgtagcgtcg tgcaaaccgt acgttcctcc ttcaacaaca ttaatgcttc
     8041 tatcagtagc attaacgcct acaaacaagc cgtagtttcc gctcaaagct cattagatgc
     8101 gatggaagcg ggctattcgg tcggtacgcg taccattgtt gatgtgttgg atgcgaccac
     8161 cacgctgtac aacgccaagc aagagctggc gaatgcgcgt tataactacc tgattaatca
     8221 gctgaatatt aagtcagccc tgggtacgtt gaacgagcag gatctgctgg cactgaacaa
     8281 tgcgctgagc aaaccggttt ccactaatcc ggaaaacgtt gccccgcaaa cgccggaaca
     8341 gaatgctatt gctgatggtt atgcgcctga tagcccggca cccgtcgttc agcaaacatc
     8401 cgcacgcact accaccagta acggtcataa ccctttccgt aactgacgac gacgacgggg
     8461 cttcggcccc gtctgaacgt aaggcaacgt aaagatacgg gttatctgcc gcattcttcc
     8521 cccttctcgc ttcaatttcg accagccatc ctctattctg atgggtattt accactggtc
     8581 ccggaagaca aaaatgaaac ggacaaaatc catacgccac gcatcgttcc gcaaaaactg
     8641 gagcgcacgc catctgacac cagtcgctct cgcggttgcc actgttttta tgctggctgg
     8701 ctgtgaaaag agtgatgaaa cagtgtctct ctatcaaaat gctgacgact gttcagctgc
     8761 aaacccaggc aaaagcgccg aatgtaccac cgcgtacaac aatgcgctga aagaagccga
     8821 acgtactgcg ccgaaatacg ccacccgtga agactgtgtt gctgaatttg gtgaaggtca
     8881 gtgccagcag gcaccagccc aggctggcat ggcaccagaa aaccaggcgc aggcccagca
     8941 atccagcggg agtttctgga tgccgctgat ggccggttac atgatgggtc gtctgatggg
     9001 cggcggcgcg ggatttgcac agcagccgct gttctcctcg aaaaacccgg ccagcccggc
     9061 ttacggtaaa tataccgacg cgaccggtaa aaactatggc gcagcccagc caggccgcac
     9121 catgaccgta ccgaagacgg caatggcacc aaaaccggcg accactacta ctgttacccg
     9181 tggcggtttt ggtgaatctg ttgccaaaca aagcactatg cagcgtagcg caaccggcac
     9241 ctcttctcgt tcaatgggtg gctgataccg atggaaagag tcagtattac cgagcgcccg
     9301 gactggcgcg aaaaagccca cgaatacggt ttcaattttc acaccatgta cggcgaaccg
     9361 tactggtgtg aagatgctta ctacaagttg accctcgccc aggttgaaaa gctggaagaa
     9421 gtcaccgccg aactgcacca gatgtgcctg aaagtggttg aaaaagtgat cgccagcgat
     9481 gagctgatga ccaaattccg cattccaaaa cacacctgga gttttgtgcg ccagtcatgg
     9541 ctgactcacc agccatcgct ttattcgcgt cttgatctgg cgtgggatgg tactggcgag
     9601 cctaaacttc tggaaaataa cgccgatacg ccaacgtcac tgtatgaagc cgcattcttt
     9661 cagtggatct ggctggaaga tcagcttaac gccggtaacc tgccggaagg cagcgaccag
     9721 tttaacagtc tgcaagagaa gctgatcgat cgctttgtcg agctgcgtga acagtatggc
     9781 ttccagttgc tgcatctcac ctgctgtcgc gacacggtgg aagatcgcgg aaccattcag
     9841 tatttgcagg actgcgcgac agaagctgaa atcgctactg aattcctcta catcgacgat
     9901 atcggtttag gcgaaaaagg tcagttcacg gatttacagg atcaggtgat ttctaacctg
     9961 ttcaagctgt atccgtggga atttatgctg cgtgagatgt tctccaccaa gctggaggat
    10021 gcaggcgtac gctggctgga accggcgtgg aagagcatta tctccaacaa agcgcttctg
    10081 ccgctactgt gggagatgtt cccgaatcac ccgaacctgc tgcctgctta ttttgcggaa
    10141 gatgatcatc cgcaaatgga aaaatatgtg gttaaaccga tcttctcccg tgaaggcgca
    10201 aacgtgtcga tcattgagaa cggcaaaacc attgaagcag cggaaggtcc gtatggcgaa
    10261 gaagggatga ttgttcagca attccacccg ttaccgaaat tcggcgacag ctatatgctg
    10321 attggtagct ggctggtgaa cgatcaacct gccggaattg gcattcgtga agaccgtgca
    10381 ttgatcaccc aggatatgtc tcggttttat ccgcatattt ttgttgagta atactgttca
    10441 atattgcccc gtttaccggg gcaatttatt atacttcgca aatcatcacc cccatctata
    10501 tataatcaat cacatccatc acttttattt aacattaatt cctaatttaa tattaaatcg
    10561 tcatatgtaa gcgatactgt aattaaatta atcaataaaa tcacttggta acctattgtt
    10621 ttataagttt atttatatcc aacaccgcat cagaaataga tttaataatt aaaaaacaat
//
LOCUS       DACBVI010000149        10678 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.150, whole genome shotgun sequence.
ACCESSION   DACBVI010000149 DACBVI010000000
VERSION     DACBVI010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.150"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            239..733
                     /gene="napF"
                     /locus_tag="IU325_18625"
     CDS             239..733
                     /gene="napF"
                     /locus_tag="IU325_18625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HAP5811020.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLLFTIGEAC
                     LAYQTVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            723..986
                     /gene="napD"
                     /locus_tag="IU325_18630"
     CDS             723..986
                     /gene="napD"
                     /locus_tag="IU325_18630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HAP5811021.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            983..3469
                     /gene="napA"
                     /locus_tag="IU325_18635"
     CDS             983..3469
                     /gene="napA"
                     /locus_tag="IU325_18635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HAP5811022.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISKPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEDLLAKKPELRGKTLYEVLYATPEVSKYPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3476..4171
                     /gene="napG"
                     /locus_tag="IU325_18640"
     CDS             3476..4171
                     /gene="napG"
                     /locus_tag="IU325_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HAP5811023.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4158..5021
                     /gene="napH"
                     /locus_tag="IU325_18645"
     CDS             4158..5021
                     /gene="napH"
                     /locus_tag="IU325_18645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HAP5811024.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            5018..5467
                     /gene="napB"
                     /locus_tag="IU325_18650"
     CDS             5018..5467
                     /gene="napB"
                     /locus_tag="IU325_18650"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HAP5811025.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5477..6079
                     /gene="napC"
                     /locus_tag="IU325_18655"
     CDS             5477..6079
                     /gene="napC"
                     /locus_tag="IU325_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HAP5811026.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6098..6715
                     /gene="ccmA"
                     /locus_tag="IU325_18660"
     CDS             6098..6715
                     /gene="ccmA"
                     /locus_tag="IU325_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HAP5811027.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGDVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            6712..7374
                     /gene="ccmB"
                     /locus_tag="IU325_18665"
     CDS             6712..7374
                     /gene="ccmB"
                     /locus_tag="IU325_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HAP5811028.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7416..8153
                     /gene="ccmC"
                     /locus_tag="IU325_18670"
     CDS             7416..8153
                     /gene="ccmC"
                     /locus_tag="IU325_18670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HAP5811029.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8150..8359
                     /gene="ccmD"
                     /locus_tag="IU325_18675"
     CDS             8150..8359
                     /gene="ccmD"
                     /locus_tag="IU325_18675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HAP5811030.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8356..8835
                     /gene="ccmE"
                     /locus_tag="IU325_18680"
     CDS             8356..8835
                     /gene="ccmE"
                     /locus_tag="IU325_18680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HAP5811031.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8832..>10678
                     /locus_tag="IU325_18685"
     CDS             8832..>10678
                     /locus_tag="IU325_18685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAP5811032.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAVLRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAG"
BASE COUNT         2190 a         2725 c         3193 g         2570 t
ORIGIN      
        1 tttctggtga attaatcgct atataaatat gtttataacc atttgaaatg tgagcaaaag
       61 cccgtttttg ccacgcttcc cgccactctt ttgatcctgc tacaggtttt accccgatcg
      121 gggtatgcat ctttgacaca tcctttaata tcttagcggc tataaaaatg gcttattaat
      181 tatgcggttt atttggtcgc tctcaatttt cagagcgcgt taatgatgga aggtcaatgt
      241 gaagattgat gcatcccgtc ggggcatact cactggtcgc tggcgcaaag ccagtaacgg
      301 tatccgtccg ccctggtccg gtgatgaatc tcattttctg acccattgca cccgctgcga
      361 cgcctgtatt aatgcttgtg aaaacaacat tctgcaacgc ggcgcgggtg gctatccgag
      421 tgttaatttc aaaaataacg agtgcagctt ctgttacgcc tgcgcgcagg cttgccccga
      481 atcgctattt tctccgcgcc acaccagggc ctgggatttg ctgttcacca tcggggaggc
      541 gtgcctggcg tatcaaacag ttgaatgccg ccgctgtcag gatagctgtg agccaatggc
      601 aattatcttt cgcccgacgc tctccgggat ctaccagccg caacttaata gccaactttg
      661 caacggatgt ggagcatgtg cagccagctg tccggtatca gccataactg cggagtatct
      721 ccatgcacac taactggcaa gtttgcagcc tggtcgtgca ggccaaaagc gaacgaattt
      781 cagacatcag cacccaactg aacgcctttc ccggctgtga agttgctgtc agcgacgcgc
      841 cgagcggtca gttgattgtg gtggtggaag cagaagacag cgaaacgctg attcaaacca
      901 ttgagtcagt acgcaacgta gagggcgtgc tggcggtgtc gctggtttat caccagcagg
      961 aagagcaagg tgaggaaaca ccatgaaact cagtcgtcgt agctttatga aagctaacgc
     1021 cgttgcggcc gctgcggcgg ctgccggtct cagcgtgccg ggcgttgccc gcgccgttgt
     1081 tggtcagcag gaagccatta aatgggataa agcgccgtgc cgtttctgcg gtactggttg
     1141 cggcgttctg gtcggaacgc agcaggggcg tgtggtggcc tgtcagggcg acccggacgc
     1201 accggttaac cgtggtctga actgcattaa gggctatttc ctgcccaaaa tcatgtacgg
     1261 taaagaccgt ttgacgcagc cgcttctgcg tatgaaaaac ggtaaatatg acaaagaagg
     1321 cgaatttacc ccaatcacct gggatcaagc cttcgatgtg atggaagaga aattcaaaac
     1381 cgccctgaaa gaaaaagggc cggaatcgat cggtatgttc ggttctggtc agtggactat
     1441 ctgggaaggt tatgccgcgt ccaagctgtt taaagcggga ttccgttcga acaacatcga
     1501 cccgaacgcg cgtcactgta tggcgtcggc ggtagttggc tttatgcgta cctttggtat
     1561 ggatgagccg atgggctgct atgacgacat cgagcaggct gatgcgtttg tgctgtgggg
     1621 cgcgaacatg gcggaaatgc acccgatcct ctggtcgcgc atcactaacc gtcgtctctc
     1681 taaccagaac gtcaccgtgg cggtgctttc tacctaccag catcgtagct tcgagctggc
     1741 ggataatggc atcatcttta cgccgcaatc agacctggtg atcctgaact acatcgccaa
     1801 ctatatcatt caaaacaatg cgataaatca ggacttcttt agcaaacacg ttaacctgcg
     1861 caaaggggcg acggacatcg gctacggttt acgtccgacc catccgctgg aaaaagcagc
     1921 gaagaatcca ggttctgacg cctccgaacc gatgagcttt gaagattaca aagccttcgt
     1981 tgctgagtat acgctggaaa aaactgccga aatgaccggc gtgccgaaag accagttaga
     2041 acaactggcg cagctgtatg ccgatccgaa caagaaagtc atctcctact ggacgatggg
     2101 cttcaaccag catacccgtg gtgtatgggc caacaacctg gtctacaacc tgcacctgct
     2161 gaccggcaaa atttccaagc cgggttgcgg tccgttctcc ctgaccgggc agccttccgc
     2221 gtgtggtact gcgcgtgaag tgggcacctt tgctcaccgt ctgcctgcgg acatggtggt
     2281 aactaacgag aaacatcgtg atatctgcga gaagaagtgg aatatcccga gcggcaccat
     2341 tccggcgaaa atcggtctgc atgcggtagc acaagaccgt gcgctgaaag acggcaagct
     2401 gaatgtttac tggaccatgt gtaccaacaa catgcaggcc gggccgaaca tcaatgaaga
     2461 gcgtatgccg ggctggcgcg atccgcgcaa cttcatcatc gtctccgatc cgtatccgac
     2521 agtcagtgcg ctggccgccg acttgatcct gccgaccgca atgtgggtag aaaaagaggg
     2581 tgcttacggt aacgccgaac gccgtactca gttctggcgt cagcaggtac aggcaccggg
     2641 cgaagcgaaa tcggatctct ggcagttagt ccagttctcc cgccgcttca aaactgaaga
     2701 agtatggccg gaagatctgc tggcgaagaa accggaactg cgtggcaaaa cgctgtacga
     2761 agttctgtat gccaccccgg aagtgagcaa ataccctgta tccgaactgg cggaagatca
     2821 gctgaacgat gaatcccgcg agctgggctt ctatctgcaa aaagggctgt tcgaagagta
     2881 cgcatggttt ggtcgcggtc acggtcacga tctggcaccg ttcgatgact accacaaagc
     2941 gcgcggtctg cgctggccgg tggtgaacgg taaagaaaca cagtggcgtt acagcgaagg
     3001 taacgacccg tacgtgaaag cgggcgaagg ctataagttc tacggtaaac cggatggcaa
     3061 agcggtgatc ttcgcactgc cgttcgaacc ggcggcggag gcaccggatg aagagtacga
     3121 cctgtggctc tctaccggac gcgtcctgga gcactggcat accggcagta tgactcgccg
     3181 tgtaccggaa ctgcaccgtg ccttcccgga agcggtcctg tttattcacc cgttggatgc
     3241 gaaagcgcgc gatctgcgcc gtggcgacaa agtgaaagtg gtttctcgcc gtggcgaagt
     3301 gatctcgatt gttgaaacgc gcggtcgtaa ccgtccgcca cagggcctgg tgtacatgcc
     3361 gttcttcgac gccgcacagc tggttaacaa actgacgctg gacgcgaccg atccgctctc
     3421 gaaagagacg gacttcaaga agtgcgcggt caaactggag aaggtgtaag ccattatgtc
     3481 ccggtcagcg aaacctcaaa atggtcgccg ccgctttctg cgcgatgtcg ttcgcacagc
     3541 tggcgggctg gctgccgtgg gtgtggcgct ggggttacaa cagcaaaccg cacgcgcatc
     3601 tggcgtgcgg ttgcgcccgc ccggagccat aaacgagaac gcctttgcca gtgcctgtgt
     3661 gcgttgcggt cagtgtgttc aggcttgccc ttacgacacc ttaaaactgg cgacgctggc
     3721 ttctggcctg tcggcgggca cgccatattt tgtcgcacgg gatattcctt gcgaaatgtg
     3781 tgaggacatt ccgtgcgcca aagtgtgccc gagcggtgcg ctggatcgtg agatcgaatc
     3841 gatcgacgac gcgcggatgg ggctggcggt actggtggac caggaaaact gtctcaactt
     3901 tcaggggctg cgctgcgatg tttgttatcg cgaatgcccg aaaattgatg aggcaatcac
     3961 cctggagctg gagcgcaaca cgcgtaccgg taagcacgcc cgctttctgc cgacggttca
     4021 cagcgacgcc tgtaccggct gcggtaagtg cgaaaaagtg tgcgtgctgg aacaaccggc
     4081 aatcaaggtg ttaccgctgt cactggcgaa aggggagtta ggtcaccatt accgcttcgg
     4141 ctggctggag gggaacaatg gcaaatcgta aacgtgacgc cgggcgcgag gcgctggaga
     4201 aaaaaggctg gtggcgcagc caccgttggc tggtgttacg tcgcctttgt cagttcttcg
     4261 tgctggggat gtttttgagc ggtccgtggt ttggtgtgtg gatcttgcac ggcaactaca
     4321 gcagtagcct gttattcgac accgttccgc tgaccgatcc gctgatgacg ctgcaaagtc
     4381 ttgccagcgg tcatctgcct gccacggtgg cgttgaccgg tgcggtaatt atcaccgtgc
     4441 tctacgccct ggcaggaaag cgattatttt gcagctgggt ctgcccgctg aacccgatta
     4501 ccgacttagc gaactggtta cgcaggcgct ttgacctcaa tcagtctgcg acgatccctc
     4561 gccacattcg ctacgtgctg ttggtggtca ttctgctggg atcggcctta accggcacac
     4621 tcatctggga atggatcaac cccgtttccc tgatggggcg cagtctggta atgggattcg
     4681 gcagcggcgc gctgcttatt ctcgcgctgt ttttatttga tctactggtc gttgaacacg
     4741 gctggtgcgg gcacatttgt ccggttggcg cgctgtatgg cgtgctgggt agcaaaggtg
     4801 tcattaccgt tgcggccacc gatcggcaga aatgtaaccg ctgtatggat tgttttcatg
     4861 tttgcccgga accgcatgtg ctacgtgccc cggtgctgga tgaacaaagc ccggtgcagg
     4921 tcaccagccg cgattgcatg acgtgcggtc gctgcgtgga tgtctgttct gaggatgtat
     4981 ttacaataac tacacgatgg agttcgggag cgaaatcatg aaaagccatg acctgaagaa
     5041 agcgctgtgt caatggacgg cgatgctggc cctggtggtg agcggcgcgg tttgggcggc
     5101 taatggcgtc gattttagcc agtcgccgga agtatccggg acgcaggaag gggccattcg
     5161 gatgccaaaa gagcaggatc ggatgccgct gaactatgtg aatcagccgc caatgatccc
     5221 gcatagcgtt gaaggttatc aggtaacgac caataccaac cgctgcttgc agtgccacgg
     5281 tgtcgaaagc tatcgcacca ctggcgcgcc gcgcatcagt cctactcact ttatggacag
     5341 cgacggcaaa gtgggcgcgg aagtggctcc gcgtcgttat ttctgtctgc aatgtcacgt
     5401 accgcaggcc gataccgcgc caatcgtggg gaataccttt accccgtcaa aaggttacgg
     5461 gaaataagag gttattatgg gaaattctga ccgtaagcct ggtctgatta agcgcctgtg
     5521 gaaatggtgg cgtactccca gccgtctggc gctggggacg ctgctgttga tcggctttgt
     5581 tggcggcatc gtcttctggg gcggctttaa caccgggatg gaaaaagcca ataccgaaga
     5641 gttctgcatt agctgccacg aaatgcgtaa cacggtgtat caggaataca tggattccgt
     5701 gcactacaac aaccgtagcg gcgttcgtgc gacctgtccg gattgtcacg ttccgcacga
     5761 gtttgtgccg aagatgatac gcaagctcaa agcaagtaaa gagctgtatg gtaaaatttt
     5821 tggcgttatt gacacgccgc agaaatttga agctcatcgc ctgacgatgg cacagaatga
     5881 gtggcggcgc atgaaggaca ataactcgca ggagtgccgt aactgtcaca acttcgagta
     5941 tatggataca accgcccaga aatcggttgc cgcgaagatg catgaccagg cggtgaaaga
     6001 tgggcaaacc tgtattgatt gccataaagg gatagcgcac aagctgcccg atatgcgcga
     6061 agtcgagcca ggtttttaac agggttattg cgtgggtatg cttgaagcca gagagttact
     6121 ttgcgagcgg gatgaacgaa ccttatttag tggcttgtca tttacgctga acgcaggaga
     6181 gtgggtacaa atcaccggta gcaacggcgc ggggaagaca acgcttctcc gtttgctgac
     6241 ggggttgtct cgccctgatg cgggtgacgt tctctggcaa gggcagccct tgcatcaggt
     6301 acgcgacagc taccatcaaa acctgttatg gataggccat cagccgggga tcaaaacccg
     6361 gctgacggcg ttagaaaatc tgcactttta tcatcgcgat ggcgataccg cacaatgtct
     6421 ggaagccctg gcgcaggccg ggcttgccgg attcgaagat attccggtaa atcaactttc
     6481 tgccgggcaa caacgccgcg tcgctttagc gcgtctgtgg ctaacccgtg ccacgttatg
     6541 gatcctcgac gagcctttta ccgcgattga cgttaacggc gtcgatcgtc tgacccagcg
     6601 tatggcgcag catacggagc agggggggat tgtgattctg actacccacc agccgctcaa
     6661 cgttgctgaa agtaaaattc gccgcatttc actgacgcaa acgagggccg catgatgttc
     6721 tggcgcattt tccgtcttga gctgcgcgta gcgtttcgcc atagcgccga aatcgccaac
     6781 ccgctgtggt tcttcctgat tgtcattacc ctttttccgc tcagtatcgg tccggagccg
     6841 caactgctgg cgcgtattgc gccgggcatt atctgggttg ctgcgctgct ttcatccttg
     6901 ctggcactgg agcgtctgtt ccgtgacgat ttgcaggacg gcagtcttga acaattgatg
     6961 ttgttgccgt tacccttgcc cgccgttgtg ctggcgaagg tgatggcgca ctggatggta
     7021 accggtctgc cgttacttat cctttcgccc ctggtagcaa tgctactggg aatggatgtt
     7081 tatggctggc aagtgatggc gctgacgcta ctgctgggaa cgcctacgct tggctttctc
     7141 ggagcaccgg gcgtggcgct gacagtggga cttaagcgcg gtggtgtgct gctcagcatt
     7201 ctcgtattac cgctgactat cccattactc atctttgcca ccgccgcgat ggacgcggct
     7261 tccatgcatt tgcccgttga tgggtatctg gcaattttag gcgcgttgct ggcaggcacc
     7321 gcgacattaa gtccttttgc gacggcggca gcgttacgaa tcagcattca ataacgctgg
     7381 attactgatt tttgttcgag tctggtatcg aaactatgtg gaaaacactg catcaactgg
     7441 cgatcccacc acggctgtat caaatctgtg gctggtttat accgtggctg gcaattgcca
     7501 gtgtggtcgt gcttaccgtt ggctggatct ggggattcgg ctttgctccg gctgattatc
     7561 agcagggaaa tagctaccgc attatctacc tgcatgtgcc tgcggcgatc tggtcgatgg
     7621 gcatttatgc atcaatggcg gtggcggcgt ttattggcct tgtctggcag atgaaaatgg
     7681 ccaacctggc ggtggcggcg atggccccca ttggtgccgt gtttaccttt attgccctgg
     7741 ttaccggctc tgcatgggga aaaccgatgt ggggcacctg gtgggtatgg gatgcacgcc
     7801 tgacttctga actggtgctg ctgtttctgt atgttggtgt aattgccctg tggcacgcct
     7861 tcgacgaccg ccgtctggcg ggccgtgcgg caggtattct ggtgctgatt ggcgtggtga
     7921 atctgccgat tattcattac tccgtggagt ggtggaacac cctgcatcag ggatcaacgc
     7981 gaatgcagca aagtatcgat ccggcgatgc gttcgccgct gcgctggtcg atttttggct
     8041 tcttgctgct gtctgccacg ctgacgctga tgcggatgcg taatttaatt ttgctgatgg
     8101 aaaaacgccg tccgtgggtg agtgaactga tactgaaaag aggccgtaaa tgacccctgc
     8161 atttgcttcc tggaatgaat ttttcgcaat gggcggttac gccttttttg tctggctggc
     8221 ggtggtgatg accgttattc cgctggtggt tttggtcgtg cactcggtga tgcagcatcg
     8281 cgcaattctg cgtggtgtgg cgcaacagcg ggcgcgtgag gcgcgtttac gtgctgcgca
     8341 acagcaggag gctgcatgaa tattcgccgt aaaaaccgct tgtggattgc ctgtgccgtg
     8401 ttggcagggc tggcgctgac tatcggtctg gtgctatatg cgctgcgctc gaatatcgat
     8461 ctcttttata cgccggggga aattctttac ggcaagcgtg aaactcagca aatgccggaa
     8521 gtcggtcagc gtctgcgcgt tggcgggatg gtgatgccgg gtagtgtgca gcgcgatccc
     8581 aattcgctaa aagtaacttt caccatttac gacgctgaag gttcagtaga tgtctcttac
     8641 gaaggcattt tgccggatct gttccgtgaa gggcagggcg ttgtggtgca gggcgaactg
     8701 gaaaaaggca atcatatcct cgcgaaagaa gtgctggcga aacatgatga aaactatacg
     8761 ccgccagaag ttgagaaagc gatggaagct aaccaccgtc gcccggcgag tgtttataag
     8821 gacccagcat catgatgccg gaaattggta acggactgct gtgcctggcg ctgggaattg
     8881 cgctgctgct gtccgtgtat ccgctatggg gcgtggcgcg cggagatgcg cgcatgatgg
     8941 cgtcttcccg cttatttgcc tggctgctgt ttatgtctgt ggctggcgca tttctggtgc
     9001 tggtcaatgc tttcgtggtc aacgacttta ccgtcaccta tgttgccagc aactctaata
     9061 cccagcttcc ggtgtggtat cgcgtggcgg ccacctgggg cgcgcatgaa ggctcgctcc
     9121 tgctgtgggt gctgctaatg agcggctgga cctttgcggt agcgattttt agtcagcgta
     9181 ttccgctgga tattgtggcc cgtgtactgg cgataatggg gatggtcagc gtcggctttt
     9241 tgctgttcat tctctttacc tctaatccat tctcacgcac gctgccgaac ttcccgattg
     9301 aagggcgcga ccttaacccg ctgttgcagg atccggggtt gatcttccat ccgcctctgc
     9361 tctatatggg gtacgtcggc ttctcggtgg cgtttgcttt tgccattgct tctttgttga
     9421 gcgggcgtct ggacagcact tatgcgcgtt ttactcgtcc gtggacactg gcagcgtgga
     9481 tcttcctgac gctcggcatc gtgctcggtt ccgcatgggc ctattacgaa ctcggctggg
     9541 gtggctggtg gttctgggat ccggtagaaa acgcctcgtt tatgccgtgg ctggtgggga
     9601 ctgcgctgat gcactcactg gcggtcactg aacaacgcgc cagcttcaaa gcgtggacat
     9661 tactgctggc aatcagtgcc ttctcgttgt gtctgttggg gactttcctc gtgcgttccg
     9721 gcgtgctggt atcggtacac gcgtttgcct ctgatccggc gcgcggcatg tttatcctcg
     9781 cctttatggt gctggtgatt ggcggttcgc tgctgctgtt tgctgcgcgt ggacacaaag
     9841 ttcgctcacg cgtaaacaat gcgctgtggt cgcgggaatc tttgctgtta gcgaacaacg
     9901 ttttgctggt cgccgcgatg ctggtggtgt tgctggggac gctgttgccg ctggtgcaca
     9961 agcaactggg actgggcagt atttcgattg gcgaaccgtt cttcaacacc atgtttacct
    10021 ggctgatggt gccgtttgcg ctactgcttg gtgtcggtcc tctggtgcgc tgggggcgcg
    10081 atcgcccacg taaaatccgc aatttattga ttatcgcctt catctctacg ctggtgctgt
    10141 ctctgctgtt gccgtggctg ttcgaaagca aagttgtggc gatgacggtg ctcggcctgg
    10201 caatggcctg ctggattgcg gtgctggcaa ttgcggaagc tgtgctgcgt atttcacgcg
    10261 gcacgaaaac caccttcagt tattggggaa tggtggcggc tcacctgggg ctggcagtga
    10321 caattgttgg tattgccttt agccagaact atagcgttga gcgtgatgtg cgcatgaagt
    10381 ccggtgatag tgtcgatatt catgaatatc gcttcacctt ccgtgatgtc aaagaggtga
    10441 ctggcccgaa ctggcgtggc ggtgtggcga ctatcggcgt aacgcgcgat ggcaagccgg
    10501 aaacggtgct gtatgcggaa aaacgttatt acaacactgc tgggtcgatg atgaccgaag
    10561 cggcaattga cggcggcatc acgcgtgacc tgtacgccgc gctcggtgaa gagctggaaa
    10621 acggcgcgtg ggccgtgcgt ctttactaca aaccgtttgt tcgctggatt tgggcggg
//
LOCUS       DACBVI010000150        10569 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.151, whole genome shotgun sequence.
ACCESSION   DACBVI010000150 DACBVI010000000
VERSION     DACBVI010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.151"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            83..1057
                     /gene="cysB"
                     /locus_tag="IU325_18690"
     CDS             83..1057
                     /gene="cysB"
                     /locus_tag="IU325_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HAP5811033.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            1388..1516
                     /locus_tag="IU325_18695"
     CDS             1388..1516
                     /locus_tag="IU325_18695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HAP5811034.1"
                     /translation="MPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKVWG"
     gene            1519..1686
                     /locus_tag="IU325_18700"
     CDS             1519..1686
                     /locus_tag="IU325_18700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811035.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            1800..1895
                     /locus_tag="IU325_18705"
     CDS             1800..1895
                     /locus_tag="IU325_18705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811036.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            2059..4734
                     /gene="acnA"
                     /locus_tag="IU325_18710"
     CDS             2059..4734
                     /gene="acnA"
                     /locus_tag="IU325_18710"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HAP5811037.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIYALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGIAEWKEIN
                     VARSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSEVISIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLIGEEKIDIVDLQSLQPGATVPVTFTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(4798..5388)
                     /gene="ribA"
                     /locus_tag="IU325_18715"
     CDS             complement(4798..5388)
                     /gene="ribA"
                     /locus_tag="IU325_18715"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HAP5811038.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            5558..6322
                     /gene="pgpB"
                     /locus_tag="IU325_18720"
     CDS             5558..6322
                     /gene="pgpB"
                     /locus_tag="IU325_18720"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HAP5811039.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAIMLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKIHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPVEENREIAQREQES"
     gene            6471..6779
                     /gene="lapA"
                     /locus_tag="IU325_18725"
     CDS             6471..6779
                     /gene="lapA"
                     /locus_tag="IU325_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HAP5811040.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVAPHSSAAKE
                     "
     gene            6786..7955
                     /gene="lapB"
                     /locus_tag="IU325_18730"
     CDS             6786..7955
                     /gene="lapB"
                     /locus_tag="IU325_18730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HAP5811041.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMALLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYALYWHCPSCRAWSTIKPIRGLDGL"
     gene            8147..8884
                     /gene="pyrF"
                     /locus_tag="IU325_18735"
     CDS             8147..8884
                     /gene="pyrF"
                     /locus_tag="IU325_18735"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP5811042.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAARFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQASAAGVDYM
                     VIGRPITQSVDPAQTLKAINASLQRSA"
     gene            8884..9210
                     /gene="yciH"
                     /locus_tag="IU325_18740"
     CDS             8884..9210
                     /gene="yciH"
                     /locus_tag="IU325_18740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HAP5811043.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(9336..9554)
                     /gene="osmB"
                     /locus_tag="IU325_18745"
     CDS             complement(9336..9554)
                     /gene="osmB"
                     /locus_tag="IU325_18745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HAP5811044.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(9823..10542)
                     /locus_tag="IU325_18750"
     CDS             complement(9823..10542)
                     /locus_tag="IU325_18750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005074625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP5811045.1"
                     /translation="MVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKLSYLRRAHGFA
                     VSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLARTLGEQKKNV
                     TIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHFSKAFIGIDGW
                     QPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVERFNRVITDSK
                     IRASDLMHLEHSKLTVHVVDI"
BASE COUNT         2634 a         2472 c         2737 g         2726 t
ORIGIN      
        1 ttttattatt aaatcgtatt agtcacccgc caggtgtgac gaaaaaacga tgttctgatg
       61 gcgtctaagt ggatggttta acatgaaatt acaacaactt cgctatattg ttgaggtggt
      121 caatcataac ctgaatgtct catcaacggc ggaaggactt tacacatcac aacccgggat
      181 cagtaaacaa gtcagaatgc tggaagacga gctaggcatt caaatttttt cccgaagcgg
      241 caagcacctg acgcaggtaa cgccagcagg acaagaaata attcgtatcg ctcgtgaagt
      301 cctgtcgaaa gttgatgcca taaaatcggt cgccggagag cacacctggc cggataaagg
      361 ctcgctgtat atcgccacca cgcataccca ggcacgctac gcgttaccaa acgtcatcaa
      421 aggctttatt gagcgttatc cccgcgtttc tttgcatatg caccagggct cgccgacaca
      481 aattgctgat gctgtctcta aaggcaatgc cgatttcgct atcgccacag aagcgttgca
      541 tctgtatgaa gatctagtga tgttaccgtg ctaccactgg aatcgggcta ttgtagtcac
      601 tccggatcac ccgctggcag gcaaaaaagc cattaccatt gaagaactgg cgcaatatcc
      661 gttggtgaca tataccttcg gctttaccgg acgttcagag ctggatactg cctttaaccg
      721 cgcagggtta acgccgcgta tcgtcttcac ggcaacggat gctgacgtca ttaaaactta
      781 cgtccggtta gggctggggg taggggtcat tgccagcatg gcggtggatc cggtcgccga
      841 tcccgacctg gtgcgcgttg atgctcacga tatcttcagc cacagtacaa ccaaaattgg
      901 ttttcgccgt agtacttttt tgcgcagtta tatgtatgat ttcattcagc gttttgcacc
      961 gcatttaacg cgtgatgtcg ttgatgcggc tgtcgcattg cgctctaatg aagaaattga
     1021 ggtcatgttt aaagatataa aactgccgga aaaataattt cttgcgttat tttcggcacc
     1081 ttttatgtag cgaaggtgcc ggaatatatt ctcttttgtt gcttatttgt ataccgtgca
     1141 caatattttt atccctcata tttatagggt aaattacctg taaagtagtg tctaattacc
     1201 cgcctcgcaa actccagttt tcaaatatct atttcaggtc aaaagattga attattcact
     1261 ggagacgatt cgtaaattcg ctggattttt gactaaagtt tctttaggat ttgtctcatc
     1321 gtatgattaa ttacaccaat cgttcaacgt ctttctgata agtgatggcg attatatgag
     1381 gttagcaatg ccttctggaa atcaggaacc ccgccgcgat cctgaattaa aacgtaaagc
     1441 ctggctggcg gtttttcttg gttctgcact tttctgggtg gttgtcgcac tgctgatttg
     1501 gaaagtgtgg ggataactat ggtcggtcag gagcaactgg agtcgtcacc attatgccag
     1561 catagtgaca atgaaacaga aacgaaacgg gaatgttccg tcgttattcc agacgactgg
     1621 caactaacat cgcagcagca agcctttata gaactgtttg ctgaagatga tcagccgaaa
     1681 caataattat catcattctt attacccatt tttaatgaat tacagggctt ttaatacacc
     1741 gcagcaataa caggttgagt tatctcaaca caaagtaata accgttaaag gtgtagccta
     1801 tgatcaacac aaatatgaaa tattggtcct ggatgggcgc attttctctg tcgatgctct
     1861 tctgggccga actcctctgg atcattactc actgatccct gaccccgctg cggcggggtt
     1921 ttcatttgtt ttgccacaag ctttctcctc ttttatcaat ttgggttgtt atcaaatcgt
     1981 tacgcgatgt ttgtgttatc tttaatattc accctgaaga gaatcagggc ttcgcaaccc
     2041 tgtcattaag gaggagctat gtcgtcaacc ctacgagaag ccagtaagga cacgttgcag
     2101 gccaaagata aaacttacca ctactacagc ctgccgcttg ctgccaaatc actgggcgat
     2161 atcacccgtt tgcctaagtc actcaaagtt ttgctcgaaa acctgctgcg ctggcaggat
     2221 ggtaactcgg ttaccgaaga ggatatctac gcgctggcgg gatggctgaa aaatgcccat
     2281 gctgaccgtg aaattgccta ccgcccggca agggtgctga tgcaggactt taccggcgta
     2341 cctgccgttg ttgatctggc ggcaatgcgc gaagcggtta aacgcctcgg cggcgatact
     2401 gcaaaggtta acccgctctc accggtcgac ctggtcattg accactcggt gaccgtcgat
     2461 cgttttggtg atgatgaggc atttgaagaa aacgtgcgcc tggaaatgga gcgcaaccac
     2521 gaacgttatg tgttcctgaa atggggaaag caggcgttca gtcgttttag cgtcgtgccg
     2581 ccaggcacag gcatttgcca tcaggttaac ctcgaatatc tcggcaaagc agtgtggagc
     2641 gaattgcagg acggtgaatg gattgcttat ccggatacac tcgttggtac tgactcgcac
     2701 accaccatga tcaacggcct tggcgtgctg gggtggggcg tgggggggat cgaagcagaa
     2761 gccgcaatgt taggtcagcc ggtttccatg cttatcccgg acgtagtggg ctttaaactc
     2821 accggaaaat tacgtgaagg tattaccgcc acagacctgg ttctcaccgt tacccaaatg
     2881 ctgcgcaaac atggcgtggt ggggaaattc gtcgaatttt atggtgatgg tctggattca
     2941 ctaccgttgg cggatcgcgc caccattgcc aatatgtcgc cagaatatgg tgccacctgt
     3001 ggcttcttcc caatcgatgc tgtcaccctc gattacatgc gtttaagcgg gcgcagtgaa
     3061 gatcaggtcg agttggtcga aaaatacgcc aaagcgcagg gcatgtggcg taacccgggc
     3121 gatgaaccga tttttaccag tacgttagaa ctggatatga atgacgttga agcgagcctg
     3181 gcagggccaa aacgcccaca ggatcgcgtt gcactgcccg atgtaccaaa agcatttgcc
     3241 gccagtaacg aactggaagt aaatgccacg cataaagatc gccagccggt cgattatgtt
     3301 atgaacggac atcagtatca gttacctgat ggcgctgtgg tcattgcagc gataacgtcg
     3361 tgcaccaata cctctaaccc aagtgtgctg atggccgcag gcttgctggc gaaaaaagcc
     3421 gtaactctgg gcctcaagcg gcaaccgtgg gtcaaagcgt cgctggcacc aggctcgaaa
     3481 gttgtttctg actatctggc aaaagcgaaa ttgacaccgt atctcgacga actggggttt
     3541 aaccttgtgg gatatggttg taccacctgt attggtaact ctgggccgct gcccgatcct
     3601 atcgaaacgg caatcaaaaa aggcgattta accgtcggtg cagtgctgtc cggcaaccgt
     3661 aactttgaag gccgtatcca tccgctggtt aaaaccaact ggctggcctc gccgccgctg
     3721 gtggttgcct atgcgctggc gggaaatatg aatatcaacc tcgcatctga gcctattggt
     3781 cacgatcgca aaggcgatcc ggtatatctg aaagatatct ggccatcggc acaagaaatt
     3841 gcccgtgcgg tagaacaagt ctccacagaa atgttccgta aagagtacgc agaagttttt
     3901 gaaggcatag cagagtggaa ggaaattaac gtcgcacgat ccgataccta cggttggcag
     3961 gaggattcaa cctatattcg cttatcgcct ttctttgatg aaatgcaggc aacaccagca
     4021 cctgtggaag atattcacgg tgcgcggatc ctcgcaatgc ttggggattc agtcaccact
     4081 gaccatatct ctccggcggg cagtattaag cccgacagcc cagcgggtcg atatctacaa
     4141 ggccggggtg ttgagcgaaa agactttaac tcctacggtt cgcggcgtgg taaccatgaa
     4201 gtgatgatgc gcggcacctt cgccaatatt cgcatccgta atgaaatggt gcctggcgtt
     4261 gaagggggga tgacgcggca tttacctgac agcgaggtca tctctattta tgatgccgcg
     4321 atgcgctata agcaggagca aacgccgctg gcggtgattg ccgggaaaga gtatggatca
     4381 ggctccagcc gtgactgggc ggcaaaaggt cctcgtctgc ttggaattcg tgtggtgatt
     4441 gccgaatcgt ttgaacgaat tcaccgttcg aatttaattg gcatgggcat cctgccgcta
     4501 gaatttccgc aaggcgtgac gcgtaaaacg ttagggctaa tcggggaaga gaagattgat
     4561 attgtcgatc tgcaaagtct acaacccggc gcgacggttc cggtgacgtt tacgcgcgcg
     4621 gatggtagcc aggaagtcgt accctgccgt tgtcgtatcg acaccgcgac ggagttgacc
     4681 tactaccaga acgacggcat tttgcattat gtcattcgta atatgttgaa gtaacgagta
     4741 tttgcttgcc ggttattttg cttccggcaa gcaaatgaat tacacaatgc aagagggtta
     4801 tttgttcagc aaatgaccca ttttctcggc tttggtatcg agataatgtt cgttattggg
     4861 gttacgacct acaatcaacg gtacgcgttc aacaatatta atccctgctt cggtcagaat
     4921 ttcgactttt ttcgggttat tggttaacaa gcggacttca ttgacgccaa ggagtttgaa
     4981 catatcagcg caaagagtga agtcgcgctc atcagcggcg aagcctaact ggtggttagc
     5041 ctctacggta tcgtaacctt gatcctgcag tgcataagcg cggattttat tcagcagacc
     5101 aatgttacga ccttcctgac ggtgatacag caaaatacca cggccttctt cggcaatttg
     5161 cgtcagtgcc gcttcgagct ggaagccaca atcgcagcgc aagctgaaca gggcgtcacc
     5221 ggtcagacat tcggaatgga cgcgcgcaag taccggggta tgcccggaaa tatcgccata
     5281 gactagcgcg acatgatcgt gtccggttgc cagttcttca aatcccacca tcaggaaatc
     5341 gccccatggg gttggcagtt tggcttctgc cacacgttta agctgcatga aattctccag
     5401 ataatgctgg ttctgtattg gcttattttg ccataacgag aaggggttca cctaatcacg
     5461 acgcgtcgat cgttcacgga atggcacaaa tctgtcaatt tttcctggaa ctggcatttt
     5521 cagttatgat tgtgggactt atcaaaaagg agaggccatg cgttcgattg ccagacgtac
     5581 cgcagtggga gctgcactat tgcttgttat gccagtagcc gtatggattt ctggctggcg
     5641 ttggcaacct ggagaacaaa gttggctact aaaagcggct ttttgggtta ctgaaactgt
     5701 cacccagccc tggggcgtca ttacacattt gattttattc ggctggtttc tctggtgtct
     5761 gcgttttcgc attaaggctg ccattatgtt atttgccatt ctggcggcgg caatccttgt
     5821 gggacaagga gttaaatcct ggatcaaaga caaagtccag gaaccacgac cttttgttat
     5881 ctggctggaa aaaatacatc atattccggt tgatgagttc tacactttaa agcgagcaga
     5941 acgcggaaat ctagtgaaag aacagttggc tgaagagaaa aatatcccac aatatttgcg
     6001 ttcacattgg caaaaagaga cggggtttgc ctttccttcc ggtcacacga tgtttgctgc
     6061 cagttgggct ctgctggccg ttggtttgct gtggccgcgt cgtcgaacgt taaccattgc
     6121 tatcttgttg gtctgggcaa cgggagtcat gggaagccgc ctgctgctcg ggatgcattg
     6181 gccacgcgat ctggtggtag ctacgttgat ttcgtgggcg ctggtggcgg tggcaacgtg
     6241 gcttgcgcaa cgaatttgtg ggccattaac accacctgtg gaagaaaatc gcgaaattgc
     6301 acaacgagaa caagaaagtt aacgctggtt gattttccga atttagccct taaatcatca
     6361 acaatgcgcg tggatgccat ttcgcagacg gcgcgaaaat ggtactttaa agggctattg
     6421 cggtaagttg accataattt attcgctcta accacataac gggaagtaat gtgaaatatt
     6481 tactcatttt cttactggtg ttagcgatct tcgtgatttc ggtcacgtta ggtgcgcaga
     6541 acgatcaaca ggtgacgttt aattatctgt tagcgcaagg ggagtaccgt atttccacat
     6601 tgctggcggt attgtttgct gcggggtttg ctatcggttg gctgatttgc ggtctgttct
     6661 ggctgcgagt tcgtgtttcc ctggcgcgcg ctgaacgtaa aataaagcga ctggaaaacc
     6721 agctttcacc cgcgactgac gtggctgtag cgccgcattc gtcagcggcg aaggaataac
     6781 tttctatgct ggagttgttg tttctgcttt tgcctgtagc cgctgcctat ggctggtata
     6841 tgggccgcag aagtgcgcaa caaaacaagc aagatgaagc caaccgcttg tcgcgtgatt
     6901 acgtagcggg ggttaacttt ctgcttagta atcaacagga taaagcggta gacctgtttc
     6961 tcgatatgct taaagaggat acgggtaccg ttgaagccca ccttacgctc ggaaacctgt
     7021 tccgttcgcg tggcgaagtt gatcgcgcta ttcgcatcca tcagacccta atggaaagcg
     7081 cctcgctgac ctatgaacag cgtctgttgg cgattcaaca actggggcgt gattacatgg
     7141 ccgccgggtt atacgaccgc gcggaagaca tgttcaatca gctgaccgat gaaactgact
     7201 tccgcattgg cgcgctgcaa cagttgctac aaatctacca ggctaccagc gagtggcaga
     7261 aagcaattga tgttgccgaa cgcctggtga agctgggtaa agataaacag cgcgtcgaaa
     7321 ttgcccattt ctactgtgag ttagccctgc agcatatggc cagcgacgat ctcgatcgtg
     7381 ccatggcctt gttaaaaaaa ggtgcggcgg cagataaaaa cagcgcccgc gtatccatca
     7441 tgatgggacg cgtatttatg gcgaaaggag aatacgccaa agccgtcgaa agtctgcaac
     7501 gcgtgatatc ccaggacaga gaactggtca gcgaaacgct ggaaatgctg caaacctgct
     7561 accagcagtt gggtaaaact gccgaatggg cagagttcct gcagcgcgcg gtggaagaga
     7621 acaccggtgc cgatgctgaa ctgatgcttg cggacatcat cgaagcgcgc gacggtagtg
     7681 aggccgcaca ggtctatatt acgcgtcagc ttcagcgtca tccgaccatg cgtgtgttcc
     7741 ataagcttat ggattaccac ttaaatgaag cggaagaagg gcgtgccaaa gagagtctga
     7801 tggtgctgcg tgacatggtt ggcgagaagg tgcgtagtaa gcctcgttat cgctgccaga
     7861 aatgtggttt taccgcatac gctctctact ggcattgtcc gtcttgtcgg gcctggtcaa
     7921 ccattaaacc gattcgcggt cttgatggcc tgtaattttt aaaaaaatcc gactttagtt
     7981 acaacatact aattgttgat gttccattgt gctccggtaa ctcaggcgca gaaaagcctg
     8041 ccaggggaga aatcgcaact gttaattttt tatttccacg ggtagaatgc tcgccgttta
     8101 cctgtttcgc gccacttccg gtgcccatca tcaagaaggt ctggtcatga cgttaactgc
     8161 ttcatcttct tcccgcgctg ttacgaattc tcctgtggtt gttgcccttg attatcataa
     8221 tcgtgatgac gcgctggcct ttgtcgacaa gatcgaccca cgcgattgtc gtctgaaagt
     8281 cggcaaagag atgtttacgc tgtttggacc tcaatttgta cgcgaacttc aacaacgggg
     8341 ttttgatatc ttcctcgacc tgaaatttca cgatattccc aacactgcag cgcacgctgt
     8401 cgctgctgca gctgacttag gcgtgtggat ggtgaatgtt catgcctccg gtggggcgcg
     8461 tatgatgacc gcagcgcgtg aggcactggt gccgtttggc aaagatgcac cgcttttgat
     8521 tgctgtgaca gtattgacca gcatggaagc cagcgacctg gccgatcttg gcgtgacact
     8581 gtcacctgca gattatgcag aacgtctggc agcactgacg caaaaatgtg gccttgatgg
     8641 tgtggtgtgt tctgctcagg aagctgcgcg ctttaaacag gtatttggtc aggagttcaa
     8701 actggttacg ccgggcattc gtccgcaggg gagtgaagct ggtgaccagc gccgcattat
     8761 gacgccagaa caggcgtcgg cggctggtgt tgattatatg gtgattggtc gcccgataac
     8821 gcaatcggta gatccagcgc agacgctgaa agcgatcaac gcctctttac agcggagtgc
     8881 atgatgagtg attccaacag ccgtctggtc tactcaacgg aaaccggacg tattgatgag
     8941 ccaaaagcgg cccctgtacg ccctaaaggc gacggtgtgg tgcgtattca gcgtcagacc
     9001 agtggacgta aaggtaaggg cgtttgcttg attaccggtg tcgatctcga tgatgccgaa
     9061 ctgacaaaac ttgcagcgga actgaagaaa aaatgcggct gcggcggagc agttaaagat
     9121 ggagttattg aaatccaggg cgataagcgt gatttattaa aatcactact tgaagcgaaa
     9181 gggatgaagg taaaactcgc aggcggttaa cataaaaagc cacggatata tccgtggctt
     9241 tcgaatattt tactgtgtgt attattcagt tatttcgtac tgaacagaat caagcgtatt
     9301 ataattgcga gtcgataatc gacatttatt tacgattatt taccgacctg gtgaccaata
     9361 acaccaccga cggcagcacc acctaatgta cccagcgtac tgccatcggt cagtactgca
     9421 ccgcctaatg cacctgcacc cgcgccaatt gcggtgttac ggtctcgttt agaccagtta
     9481 gaacaggcac tcagagacat tgccagggta attgccagaa cagccgcggt catttttttg
     9541 ctcgttacaa acataatact ctctcctgaa tttatgattc acggaagtaa gctcactata
     9601 actataatag ctaagaataa gcctggtgaa attattccgt gaaatccgct cgcgcgcagg
     9661 gttatatcac gcaggtgata atgacttcct gttatatcgc tgataataat tttatatctt
     9721 gagagtgtta ataacaggta aatagtctta attatcaacc aggaatcatc ttagagcgga
     9781 tgatttgcaa aacagcaaat catccgtaga gaagggaaat ggttaaatgt caacgacgtg
     9841 aacggtgagt ttactgtgct caagatgcat cagatcgctg gcgcgtattt tcgaatcggt
     9901 aatcacgcga ttgaatcgct caacggggcc gatggagtat gaatgtacag caccaaattt
     9961 cgagctgtca gtcaggacta tcgcttcgca ctctttttcc agcacggcat tgaccacatc
    10021 ggtacgcatc atatcgcgac cggtaaaccc agtttcaggt tgccagccat caataccaat
    10081 aaatgctttg ctgaaatgca cctgttggat gcactggcgt gtcaaaggac caaccatact
    10141 ttcgcttttt ttctggtaca cgccaccgag caaaataact tcacaaggcg cgtctttcag
    10201 caaatgcgcg atgtagctgc tgaccgtgat gatagtgaca tttttcttct gctcgcccag
    10261 agtccgggcg agcagggcat tgctgctgcc attttcgata aagatggttt cgcccggttg
    10321 aaccagtgac gcggcaaact cggcgagttc acgcttcagc gtatagttgc tcatcatacg
    10381 agtttcgacg tcatcactat caagcgatac tgcaaagcca tgtgcacggc ggaggtaact
    10441 cagtttttcg agggtgttga gatcctggcg aatggtaact tcagaaactc cagtggcttt
    10501 tgccagatcg gttacgctaa cctgaccctg gtcaatgacc atctgtagaa ttgtttgttg
    10561 tcgggaatt
//
LOCUS       DACBVI010000151        10446 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.152, whole genome shotgun sequence.
ACCESSION   DACBVI010000151 DACBVI010000000
VERSION     DACBVI010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.152"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            144..2012
                     /locus_tag="IU325_18755"
     CDS             144..2012
                     /locus_tag="IU325_18755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HAP5811046.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2179..2598
                     /gene="rbsD"
                     /locus_tag="IU325_18760"
     CDS             2179..2598
                     /gene="rbsD"
                     /locus_tag="IU325_18760"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP5811047.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2606..4111
                     /gene="rbsA"
                     /locus_tag="IU325_18765"
     CDS             2606..4111
                     /gene="rbsA"
                     /locus_tag="IU325_18765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HAP5811048.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFAREQATQEVLMAAAVGKLNRVNQE"
     gene            4116..5081
                     /gene="rbsC"
                     /locus_tag="IU325_18770"
     CDS             4116..5081
                     /gene="rbsC"
                     /locus_tag="IU325_18770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAP5811049.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5106..5996
                     /gene="rbsB"
                     /locus_tag="IU325_18775"
     CDS             5106..5996
                     /gene="rbsB"
                     /locus_tag="IU325_18775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HAP5811050.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            6122..7051
                     /gene="rbsK"
                     /locus_tag="IU325_18780"
     CDS             6122..7051
                     /gene="rbsK"
                     /locus_tag="IU325_18780"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP5811051.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDISPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWVSVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7055..8047
                     /gene="rbsR"
                     /locus_tag="IU325_18785"
     CDS             7055..8047
                     /gene="rbsR"
                     /locus_tag="IU325_18785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HAP5811052.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8013..9440)
                     /locus_tag="IU325_18790"
     CDS             complement(8013..9440)
                     /locus_tag="IU325_18790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HAP5811053.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9463..10155)
                     /locus_tag="IU325_18795"
     CDS             complement(9463..10155)
                     /locus_tag="IU325_18795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP5811054.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMVALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2561 a         2523 c         2769 g         2593 t
ORIGIN      
        1 caaagggggg gtatttttct ttgagccagg ttaatgtggc cgcatttagg agtacgattt
       61 tgccgttaat cgtgcatact gtgcgctttt ttgtgggcca agggactaag cacacatttc
      121 atatttcaac gaaagactag tctatgagca ctgataataa gcaatcattg cccgcgatta
      181 ccctcgcggc gattggagtt gtctacggcg atattggtac cagcccgtta tatacacttc
      241 gtgaatgttt gtccggccag tttggttttg gcgttgaacg cgatgccgtg tttggctttt
      301 tatcgctgat cttctggctg ctaatctttg tggtttccat taaatatctc accttcgtga
      361 tgcgggcaga taacgccggt gaagggggga tcctgacgtt gatgtcgctt gccgggcgca
      421 atacgtcggc gcgaaccaca tcaatgctgg tgattatggg gctaatcggc ggcagctttt
      481 tctatggtga agtcgtcata acacccgcta tttcggtgat gtcagccatt gaaggtctgg
      541 aaatcgtcgc cccgcagctg gatacctgga tagttcctct ctcaattatc gttctcactt
      601 tattatttat gattcaaaag catggcacgg ctatggtcgg taagctgttt gcgccgatca
      661 tgctgacctg gtttttgatt ctggctgggc tggggttacg tagcattatt gctaacccgg
      721 aagtgctgca tgcgctgaat ccgatgtggg cggtgcattt cttccttgaa tacaaaacgg
      781 tttcttttat tgcattaggg gcagtggtgc tgtcgattac gggggtcgag gcgctgtatg
      841 ctgatatggg gcactttggt aagttcccga ttcgtctggc gtggttcacc gtcgtattgc
      901 cttccttaac tcttaattac ttcggtcagg gagcgctgtt gttaaagaac ccggaagcaa
      961 ttaagaaccc gttcttcctg ttggcaccgg actgggcgct gatcccgctg ctgatcatcg
     1021 ccgcactggc gacggtaatc gcctcgcagg ctgttatctc tggcgtcttc tcattgacgc
     1081 gtcaggcggt acgtctggga tatttgtcgc cgatgcgcat tattcacacc tccgaaatgg
     1141 agtcagggca aatctatatt ccgtttgtga actggatgct ctatgtcgcg gtcgtgattg
     1201 tgattgtcag ctttgagcac tccagcaacc tggcggcggc gtacgggatt gcggtgaccg
     1261 gaaccatggt gctgacgtct attctctcga ctaccgtggc acgtcagaac tggcactgga
     1321 ataagtattt tgttgcgctg atcctgattg ctttcctttg tgtcgatatt ccattgttca
     1381 ccgctaacct cgataaactg ctctccggcg gctggttgcc attgagcctc ggtacagtga
     1441 tgtttatcgt gatgaccacc tggaagagcg agcgtttccg cttgctgcgg cggatgcatg
     1501 aacatggcaa ctctctggag gcgatgattg cttcgctgga gaaatcaccg cccgttcgcg
     1561 tgcccgggac cgcggtgtat atgtcacgtg caatcaacgt cattcccttt gcgctgatgc
     1621 ataacctgaa acataacaag gtattgcatg agcgggtgat tctgttaact ctgcgcaccg
     1681 aagacgcgcc atatgtccat aacgtccgtc gggtacagat tgaacaactg tcgcccactt
     1741 tctggcgcgt ggtggcaagt tatggttggc gagaaacgcc aaatgtggaa gaagtattcc
     1801 accgctgcgg tctggaagga ttaagttgcc ggatgatgga aacctccttc tttatgtcgc
     1861 atgagtcgtt gatcctcggc aaacgcccgt ggtatttgcg tctgcgcggt aagctgtact
     1921 tgctgttgca acgtaatgcg ctgcgtgcgc cggatcaatt tgaaatcccg ccaaacaggg
     1981 ttatcgaact gggtactcag gtcgaaatct aacgccagac gcctcctttc tccataaggg
     2041 ggcgtttttg ttttcatggt taatcaccat gtaaaacgtt tcgaggttga tcacatttcc
     2101 gtaacgtcac gatggttttc ccaactcagt caggattaaa ctgcgggtca gcgaaacgtt
     2161 tcgctgatgg agaaaaaaat gaaaaaaggc accgttctta attctgatat ttcatcggtg
     2221 atctcccgac tgggacatac cgatacgctg gtggtgtgtg atgcaggttt acccatcccc
     2281 aaaagtacaa cgcgtatcga tatggcatta acccagggtg taccttcttt tatgcaggtg
     2341 ctgggcgtcg tcacaaatga aatgcaggtc gaggcagcca ttatcgcgga agagatcaaa
     2401 caacataatc cgcaactcca cgaaacgttg ctcactcacc ttgagcagct gcaaaaacac
     2461 cagggaaata ccattgaaat tcgttacacc acgcatgaac aattcaaaca acaaaccgca
     2521 gaaagtcagg cggtaattcg cagcggagaa tgttctccgt atgcgaatat cattctctgt
     2581 gctggcgtga cgttctgagg ccgtcatgga agcattactt cagcttaaag gcatcgataa
     2641 agccttcccg ggcgtaaaag ccctctcggg tgcagcgtta aatgtctatc ctggccgcgt
     2701 gatggcgctg gtgggcgaaa acggtgcggg taaatccacc atgatgaaag tgcttacggg
     2761 tatctatact cgcgatgccg gtacgctttt atggctgggg aaagaaacga cgtttaccgg
     2821 gccgaaatct tcccaggaag ccgggattgg gattatccat caggaactga acctgatccc
     2881 gcagttgacc attgccgaaa acattttcct cggtcgtgag tttgttaatc gctttggcaa
     2941 aattgactgg aaaaccatgt atgccgaagc ggataaattg ctggctaaac ttaacctgcg
     3001 ctttaaaagc gacaagttag tgggcgatct ttccatcggt gaccagcaaa tggttgaaat
     3061 agccaaagtg ctgagctttg agtcgaaagt catcattatg gatgaaccga ccgatgcgct
     3121 gaccgatacc gaaaccgaat ccttgttccg cgtcatccgc gagctgaaat cgcaaggccg
     3181 cggtattgtc tatatttccc accgcatgaa agaaatcttc gagatttgcg atgacgttac
     3241 cgtttttcgt gatgggcaat ttattgctga gcgtgaagta gcatcactga ccgaagattc
     3301 gctgattgag atgatggtgg gccgcaagct ggaagatcaa tatccacacc tggacaaagc
     3361 gccaggagat attcgcctga aagtcgataa tctctgcgga cctggcgtta acgatgtctc
     3421 ttttacttta cgcaaaggcg aaattcttgg cgtctctggt ttgatgggcg ctgggcgtac
     3481 cgaactgatg aaagtgctct acggcgcact gccgcgcacc agcggttacg tcaccctgga
     3541 tgggcatgaa gtcgttaccc gttcaccgca ggatggcctg gcaaacggca ttgtgtatat
     3601 ctctgaagac cgtaaacgcg acggtttggt gctgggcatg tcagtaaaag agaacatgtc
     3661 gctgacagcg ctgcgctact tcagccgcgc tggcggcagt ttgaagcatg ccgatgaaca
     3721 gcaggcagta agtgatttca ttcgtctgtt taatgtgaaa acgccgtcga tggaacaggc
     3781 aattggtctg ctttccggtg gcaatcagca aaaagtggcg attgcccgcg gtctgatgac
     3841 acgccccaaa gtgttgatcc tcgatgagcc tacccgtggc gtagatgtcg gcgcgaaaaa
     3901 agagatctat caactgatta accagttcaa agccgatggc ttgagcatca ttctggtgtc
     3961 atcggagatg ccagaagtat taggcatgag cgatcgcatc atcgtcatgc atgaagggca
     4021 tctcagcggg gaatttgctc gtgagcaggc cacccaagaa gtgttaatgg ctgccgctgt
     4081 gggcaagctt aatcgcgtga atcaggagta aaaaaatgac aacccagact gtctctggtc
     4141 gccgttattt cacgaaagcg tggctgatgg agcagaaatc gcttatcgct ctgctggtgc
     4201 tgatcgcgat tgtctcgacg ttaagcccga actttttcac catcaataac ttattcaata
     4261 ttctccagca aacctcggtg aacgccatta tggcagtcgg gatgacgctg gtgatcctga
     4321 cgtcgggcat cgacttgtcg gtaggttctc tattggcgct gaccggcgca gttgctgcat
     4381 ctatcgttgg cattgaagtc aatgcgctgg tggctgtcgc tgccgctctc gcgttaggtg
     4441 ccgcaattgg tgcggtaacc ggggtgattg tagcgaaagg tcgcgtccag gcgtttatcg
     4501 ctacgctggt tatgatgctt ttactgcgcg gcgtgaccat ggtttatacc aacggtagcc
     4561 cagtgaatac cggctttact gagaacgccg atctgtttgg ctggtttggg atcggtcgtc
     4621 cgctgggcgt accgacgcca gtctggatca tggggattgt gttcctcgcg gcctggtaca
     4681 tgctgcatca cacgcgtctg gggcgttaca tctacgcgct gggcggcaac gaagcggcaa
     4741 cgcgcctttc tggtatcaac gtcaataaaa tcaaaatcat cgtctattct ctttgtggtc
     4801 tgctggcatc gctggccggg atcattgaag tggcgcgtct ctcctccgca caacctacgg
     4861 cggggactgg ctatgagctg gatgctattg ctgcggtggt tctgggcggt acgagtctgg
     4921 cgggcggaaa aggtcgcatt gttgggacgt tgatcggcgc attaattctt ggcttcctta
     4981 ataatggatt gaatttgtta ggtgtttcct cctattacca gatgatcgtc aaagcggtgg
     5041 tgattttgct ggcggtgctg gtagacaaca aaaagcagta ataacgacta caggacatct
     5101 tgagtatgaa catgaaaaaa ctggctaccc tggtttccgc tgttgcgcta agcgccaccg
     5161 tcagtgcgaa tgcgatggca aaagacacca tcgcgctggt ggtctccacg cttaacaacc
     5221 cgttctttgt atcgctgaaa gatggcgcgc agaaagaggc ggataaactt ggctataacc
     5281 tggtggtgct ggactcccag aacaacccgg cgaaagagct ggcgaacgtg caggacttaa
     5341 ccgttcgcgg cacaaaaatt ctgctgatta acccgaccga ctccgacgca gtgggtaatg
     5401 ctgtgaagat ggctaaccag gcgaacatcc cggttatcac tcttgaccgc caggcaacga
     5461 aaggtgaagt ggtgagccac attgcttctg ataacgtact gggcggcaaa atcgccggtg
     5521 attacatcgc gaagaaagcg ggtgaaggtg ccaaagttat cgagctgcaa ggcattgccg
     5581 gtacatccgc agcccgtgaa cgtggcgaag gcttccagca ggctgttgct gctcacaagt
     5641 ttaatgttct tgccagccag ccagcagatt ttgatcgcac taaaggtttg aacgtaatgc
     5701 agaacctgtt gaccgcacat ccggatgttc aggctgtatt cgcgcagaat gatgaaatgg
     5761 cgctgggcgc gctgcgcgca ctgcaaactg ccggtaaatc ggatgtgatg gtcgtcggat
     5821 ttgacggtac accggatggc gaaaaagcgg tgaatgatgg caaactagca gcgactatcg
     5881 ctcagctacc cgatcagatt ggcgcgaaag gcgttgaaac cgcagataaa gtgctgaaag
     5941 gcgagaaagt tcaggctaag tatccggttg atctgaaact ggttgttaag cagtagtttt
     6001 aatcaggttg tatgacctga tggtgacata aatacgtcat cgacagatga acgtgtaata
     6061 taaagaaaag cagggcacgc gccaccctaa cacggtggcg cattttatgg acatcccgaa
     6121 tatgcaaaac gcaggcagcc tcgttgttct tggcagcatt aatgctgacc acattctgaa
     6181 tcttcaatct tttcctactc caggcgaaac cgtaaccggt aaccactatc aggttgcatt
     6241 tggcggcaaa ggcgcgaatc aggctgtggc tgctgggcgt agcggtgcga atatcgcgtt
     6301 tattgcctgc actggcgatg acagcattgg tgagagcgtt cgccagcagc tcgccactga
     6361 taacatcgat atttctccgg tcagcgtgat caaaggcgaa tcaacaggtg tggcgctgat
     6421 ttttgttaat ggcgaaggtg agaatgtcat cggtattcat gccggcgcta atgctgccct
     6481 ttccccggcg ctggtggaag cgcaacgtga gcgtattgcc aacgcgtcag cattattaat
     6541 gcagctggaa tcaccactcg aaagtgtgat ggcagcggcg aaaatcgccc atcaaaataa
     6601 gactatcgtt gcgcttaacc cggctccggc tcgcgaactt cctgacgaac tgctggcgct
     6661 ggtggacatt attacgccaa acgaaacgga agcagaaaag ctcaccggta ttcgtgttga
     6721 aaatgatgaa gatgcagcga aggcggcgca ggtactgcat gaaaaaggta tccgtactgt
     6781 actgattact ttaggaagtc gtggtgtatg ggttagcgtg aatggtgaag gtcagcgcgt
     6841 tcctggattc cgggtgcagg ctgtcgatac cattgctgcc ggagatacct ttaacggcgc
     6901 gttaatcacg gcgttgctgg aagaaaaacc attgccagag gcgattcggt ttgcccatgc
     6961 cgccgctgcg attgccgtaa cacgtaaagg cgcacaacct tccgtaccgt ggcgtgaaga
     7021 gatcgacgca tttttagaca ggcagaggtg acgcttggct acaatgaaag atgttgcccg
     7081 cctggcgggc gtttctacct caacagtttc tcacgttatc aataaagatc gcttcgtcag
     7141 tgaagcgatt accgccaaag ttgaagcggc gattaaagaa ctcaattacg cgccatcagc
     7201 cctggcgcgt agcctcaaac tcaatcaaac acataccatt ggcatgttga tcactgccag
     7261 taccaatcct ttctattcag aactggtgcg tggcgttgaa cgcagctgct tcgaacgcgg
     7321 ttatagtctc gtcctttgca ataccgaagg cgatgaacag cggatgaatc gcaatctgga
     7381 aacgctgatg caaaaacgcg ttgatggctt gctgttactg tgcaccgaaa cgcatcaacc
     7441 ttcgcgtgaa atcatgcaac gttatccgac agtgcctact gtgatgatgg actgggctcc
     7501 gttcgatggc gacagcgatc ttattcagga taactcgttg ctgggcggag acttagcaac
     7561 gcaatatctg atcgataaag gtcatacccg tatcgcctgt attaccggcc cgctggataa
     7621 aactccggcg cgcctgaggt tggaaggtta tcgggcggcg atgaaacgtg cgggtctcaa
     7681 cattcctgat ggctatgaag tcactggtga ttttgaattt aacggcgggt ttgacgctat
     7741 gcgccaactg ttatcacacc cgctgcgtcc tcaggccgtc tttaccggaa atgacgctat
     7801 ggctgttggc gtttaccagg cgctgtatca ggcagagtta caggttccgc aggatatcgc
     7861 ggtgattggc tatgacgata tcgaactggc aagctttatg acgccaccat taaccactat
     7921 ccaccaaccg aaagatgaac tgggggagct ggcgattgat gtactcatcc atcggataac
     7981 ccaaccgacc cttcagcaac aacgattaca acttactccg attctgatgg aacgcggttc
     8041 ggcttagatt tacgctgtct tttgatcaaa ttattaccat cggttgtttt cagaagcatg
     8101 aacattgctg ctgaagcaac agtaataatg cccattgtga taaacgtata gtggaattgt
     8161 tcgacagtcg ttgtaccttc catcccttca taaacgcgaa ggacggccgc acttacagca
     8221 acgcctaaac tgatagacag ttgctgcgtg accgccagaa cactgttacc gctgctggcg
     8281 ttgtcatcgg tcagatcggc aagtgtgatg gtattcattg cggtaaattg cgtcgacata
     8341 gccatcccta atataaacaa cggcaagatc agcatccata tcgccattgc tggtgattgc
     8401 aaagagaact gagcgatcat tagcccaata atcaccgtga tccccactaa cgtatggcga
     8461 tagcccagac gacgtaagac ttgggtaacc atcgattttg caataatgga acctaacgct
     8521 gtcggtgcca tcatacagcc ggcaataaac gcctgataac caaatcctac ctgtaacatc
     8581 aatggcataa ggaacggtac acagccggtc cccagacggg ttgcaatatt gcctacgata
     8641 ccgatcgaga aagtgcgggt tttaaataaa tctaatgaaa ttaatgggtt tggcgtgcgt
     8701 cgtgcatgga gaatatagag aagcagtaac ccgatgctgg tgacaattac cgtcaaggca
     8761 atccaactgg cgacaatctt ttccccgaat agctctattc cgcttgagaa gagaacaagg
     8821 ctaaggccaa acagcaaaaa gccagtgata tcgaatctgc gtcgtgcggt ggtgaaattg
     8881 ggcatatgtt tgcgcgcgta aagaaggccc gcaataccta tggggatatt gattaaaaat
     8941 atccagtgcc aggttgccca ggtgaccagc acgccgccaa gaacggggcc taaaattggc
     9001 cccaccagac ccggcatggc gacaaaattc aatacaggaa gaagctcatt acgaggataa
     9061 gcgcgcagta aggccagccg agcaacaggc atcatcattg cgccgcctat cccctgaata
     9121 acccggaaga caaccagctg tggtagcgag ttagaaagtg cgcaggccag agaacccaac
     9181 gtgaacagac tcacggcaag ggtaaatatg cgacgcgtac cgaagcgatc ggctagccat
     9241 ccgcttaccg gaataagcat cgccaccgtc agcgtataac tgatgatggc tgattgcatc
     9301 gcgagaggag aacgattaag gctatgagcg attgcgggta aggcggtatt cagaatagtg
     9361 gcatcaagtg cctgcatgaa gaaggccatc gccgcgatcc acggcaaacc cgccatactg
     9421 cgcttctttt tatcgctcat tcaatgtcct gttatcgggt tgtcacttat caggtgagcg
     9481 tagcagcgcc tgacaagctt taaatgccgc gtcgccatcg ctttggacaa tcgcatcgac
     9541 aatcgcctgg tgcagatcca gctttatcac tgtgtcgctg gtaattgacg tgaagtaagt
     9601 gtgataaacc gaatggaata gcgaggcgaa tgaggtcaaa aacggattgg cgctcatttc
     9661 atagatatgc tcatgccaag ccatatcgac ctcaatccag cgttcacggc gaaagttctc
     9721 ttttaatgcc accatttcgg ccattaacgt attgagatgc gccttctgtt ccgcggtgcc
     9781 aaccgttgca gccagtaggc aagcttgcgg ctccagacag atacgcataa ccagaaagtg
     9841 atcgatgacc tgatgaaagt tctcttctgt catccaccag gtaagcaatt cctgatcaag
     9901 aaaattccag tttgattgtg gcatgacccg ggtaccaatt cgcggtcgcg gtaaaaccat
     9961 cccttttgcc gttaacgttt tgaccgcttc gcgtaccgct gtacgactca ctccaaattg
    10021 ctcgcccagc tcaatctcac caggcaaaat ggtgccgggt tcatattcac cttttaagat
    10081 gcgttgcgcc agcttctcag ccagaacata cgaaaggttt ttctgtgcag ctaactgttg
    10141 tgcgcttaaa ggcattacta atcttccttt ttctttttat tcctccttag tatgccacca
    10201 ggaagtgtga ttacggttgc aaaaacggca aattgcttgt tttatggcac attaacgggg
    10261 cttttgctga aaaaatgcgc ggtcagaaaa ttattttaaa tttcctcttg tcaggccgga
    10321 ataactccct ataatgcgcc accactgaca cggaacaacg gcaaacacgc cgccgggtca
    10381 gcggggttct cctgagaatc tcaacagaga aagcaaaaat aaatgcttga ctctgtagcg
    10441 ggaaag
//
LOCUS       DACBVI010000152        10428 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.153, whole genome shotgun sequence.
ACCESSION   DACBVI010000152 DACBVI010000000
VERSION     DACBVI010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.153"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            257..661
                     /gene="sdhC"
                     /locus_tag="IU325_18800"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             257..661
                     /gene="sdhC"
                     /locus_tag="IU325_18800"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAP5811055.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            655..1002
                     /gene="sdhD"
                     /locus_tag="IU325_18805"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             655..1002
                     /gene="sdhD"
                     /locus_tag="IU325_18805"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HAP5811056.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            1002..2768
                     /gene="sdhA"
                     /locus_tag="IU325_18810"
     CDS             1002..2768
                     /gene="sdhA"
                     /locus_tag="IU325_18810"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HAP5811057.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNADGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLERLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            2784..3500
                     /gene="sdhB"
                     /locus_tag="IU325_18815"
     CDS             2784..3500
                     /gene="sdhB"
                     /locus_tag="IU325_18815"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HAP5811058.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            3850..6651
                     /gene="sucA"
                     /locus_tag="IU325_18820"
     CDS             3850..6651
                     /gene="sucA"
                     /locus_tag="IU325_18820"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HAP5811059.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            6666..7883
                     /gene="odhB"
                     /locus_tag="IU325_18825"
     CDS             6666..7883
                     /gene="odhB"
                     /locus_tag="IU325_18825"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HAP5811060.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSTGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            7977..9143
                     /gene="sucC"
                     /locus_tag="IU325_18830"
     CDS             7977..9143
                     /gene="sucC"
                     /locus_tag="IU325_18830"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HAP5811061.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            9143..10012
                     /gene="sucD"
                     /locus_tag="IU325_18835"
     CDS             9143..10012
                     /gene="sucD"
                     /locus_tag="IU325_18835"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HAP5811062.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEVLKTVLK"
BASE COUNT         2470 a         2708 c         2907 g         2343 t
ORIGIN      
        1 tatatgtagg ttaattgtaa tgattttgtg aacagcctat actgccgcca ggtctccgga
       61 acaccctgca atcccgagcc acccagcgtt gtaacgtgtc gttttcgcat ctggaagcag
      121 tgttttgcat gacgcgcagt tatagaaagg acgctgtctg acccgcaagc agaccggagg
      181 aaggaaatcc cgacgtctcc aggtaacaga aagttaacct ctgtgcccgt agtccccagg
      241 gaataataag aacagcatgt gggcgttatt catgataaga aatgtgaaaa aacaaagacc
      301 tgttaatctg gacctacaga ccatccggtt ccccatcacg gcgatagcgt ccattctcca
      361 tcgcgtttcc ggtgtgatca cctttgttgc agtgggcatc ctgctgtggc ttctgggtac
      421 cagcctctct tcccctgaag gtttcgagca agcttccgcg attatgggca gcttcttcgt
      481 caaatttatc atgtggggca tccttaccgc tctggcgtat cacgtcgtcg taggtattcg
      541 ccacatgatg atggattttg gctatctgga agaaacattc gaagcgggta aacgctccgc
      601 caaaatctcc tttgttatta ctgtcgtgct ttcacttctc gcaggagtcc tcgtatggta
      661 agcaacgcct ccgcattagg acgcaatggc gtacatgatt ttatcctcgt tcgcgctacc
      721 gctatcgtcc tgacgctcta catcatttat atggtcggtt ttttcgctac cagtggcgag
      781 ctgacatatg aagtctggat cggtttcttc gcctctgcgt tcaccaaagt gttcaccctg
      841 ctggcgctgt tttctatctt gatccatgcc tggatcggca tgtggcaggt gttgaccgac
      901 tacgttaaac cgctggcttt gcgcctgatg ctgcaactgg tgattgtcgt tgcactggtg
      961 gtttacgtga tttatggatt cgttgtggtg tggggtgtgt gatgaaattg ccagtcagag
     1021 aatttgatgc agttgtgatt ggtgccggtg gcgcaggtat gcgcgcggcg ctgcaaattt
     1081 cccagagcgg tcagacctgt gcgctgctct ctaaagtctt cccgacccgt tcccataccg
     1141 tttctgcgca aggcggcatt accgttgcgc tgggtaatac ccatgaagat aactgggaat
     1201 ggcatatgta cgacaccgta aaaggttcgg actatatcgg tgaccaggac gcgattgaat
     1261 atatgtgtaa aaccgggccg gaagcgattc tggagctcga acacatgggc ctgccgttct
     1321 cgcgccttga tgatggtcgt atctatcaac gtccgtttgg cggccagtcg aaaaacttcg
     1381 gcggcgagca ggcggcacgc actgcggcgg cagctgaccg taccggtcac gcactgttgc
     1441 acacccttta tcagcagaac ctgaaaaacc acaccactat tttctccgaa tggtatgcgc
     1501 tggatctggt gaaaaacgcc gatggcgcgg tggtcggttg taccgcactg tgcatcgaaa
     1561 ctggtgaagt ggtttatttc aaagcccgcg ctaccgtgct ggcgactggc ggggcagggc
     1621 gtatttatca gtccaccacc aacgcccaca ttaacactgg cgacggcgtc ggcatggcta
     1681 tccgtgcagg cgtaccagta caggatatgg aaatgtggca gttccacccg accggcattg
     1741 caggagcggg cgtactggtc accgaaggtt gccgtggtga aggcggttat ctgctgaaca
     1801 aacatggcga acgctttatg gagcgttatg cgccgaacgc caaagacctg gcgggccgtg
     1861 acgtggtggc gcgttccatc atgatcgaaa tccgtgaagg tcgcggctgt gatggtccgt
     1921 gggggccaca cgcaaaactg aaacttgacc acctgggtaa agaagttctc gaatcccgtc
     1981 tgccgggtat cctggaactc tcccgtacct tcgctcacgt tgatccggtg aaagagccga
     2041 ttccggttat cccaacctgt cactacatga tgggcggtat tccgaccaaa gtgaccggtc
     2101 aggcgctgac tgtgaacgag aaaggcgaag atgtggttgt tccggggctg tttgccgttg
     2161 gtgaaatcgc ttgtgtatcg gtacacggcg ctaaccgtct gggcggcaac tcgctgctgg
     2221 acctggtggt ctttggtcgt gcggcaggtc tgcatctgca agagtctatc gccgagcagg
     2281 gcgcactgcg cgatgccagc gagtctgatg ttgaagcgtc tctggagcgt ctgaaccgct
     2341 ggaacaataa tcgtaacggt gaagatccgg tggcgattcg taaagcgctg caagaatgta
     2401 tgcagcataa cttctcggtc ttccgtgaag gtgatgcgat ggcgaaaggg cttgagcagt
     2461 tgaaagtgat ccgcgagcgt ctgaaaaatg cccgtctgga tgacacttcc agcgagttca
     2521 acacccagcg cgttgagtgc ctggaactgg ataacctgat ggaaacggcg tatgcaacgg
     2581 ctgtttctgc caacttccgt accgaaagcc gtggcgcgca tagccgcttc gacttcccgg
     2641 atcgtgatga tgaaaactgg ctgtgccact ccctgtatct gccagagtcg gaatccatga
     2701 cgcgccgaag cgtcaacatg gaaccgaaac tgcgcccggc attcccgccg aagattcgta
     2761 cttactaatg cggagacagg aaaatgagac tcgagttttc aatttatcgc tataacccgg
     2821 atgttgatga tgctccgcgt atgcaggatt acaccctgga agcggaagaa ggtcgtgaca
     2881 tgatgctgct ggatgcgctt atccagctga aagagaaaga tcccagcctg tcgttccgtc
     2941 gctcctgccg tgaaggtgtg tgcggttccg acggtctgaa catgaacggc aagaatggtc
     3001 tggcctgtat taccccgatt tcggcactca accagccggg caagaagatt gtgattcgcc
     3061 cgctgccagg tttaccggtg atccgcgatt tggtggtaga catgggacaa ttctatgcgc
     3121 aatatgagaa aattaagcct tacctgttga ataatggaca aaatccgcca gctcgcgagc
     3181 atttacagat gccagagcag cgcgaaaaac tcgacgggct gtatgaatgt attctctgcg
     3241 catgttgttc aacctcttgt ccgtctttct ggtggaatcc cgataagttt atcggcccgg
     3301 caggcttgtt agcggcatat cgtttcctga tcgatagccg tgataccgag actgacagcc
     3361 gcctcgacgg tttgagcgat gcattcagtg tattccgctg tcacagcatc atgaactgcg
     3421 tcagtgtatg tccgaagggg ctgaacccga cgcgcgccat cggccatatc aagtcgatgt
     3481 tgttgcagcg taatgcgtaa acccaggcct gataagacgc acaagcgtcg catcaggcaa
     3541 ccagtgccgg atgcggcgtg aacgccttat ccggcctaca aaagaaatgc agaaatcgta
     3601 ggcctgataa gacgcgcaag cgtcgcatca ggcaaacagc gccggatgcg gcgtgaatgc
     3661 cttatccggc ctacaaaaga aatgcaggaa atctttaaaa actgcccctg acactaagac
     3721 agtttttaaa ggttccttcg cgagccacta cgtagacaag agctcgcaag tgaaccccgg
     3781 cacgcacatc actgtgcgtg gtagtatcca cggcgaagta agcataaaaa agatgcttaa
     3841 gggatcacga tgcagaacag cgctttgaaa gcctggttgg actcttctta cctctctggc
     3901 gcaaaccaga gctggataga acagctctat gaagacttct taaccgatcc tgactcggtt
     3961 gacgctaact ggcgttcgac gttccagcag ttacctggta cgggagtcaa accggatcaa
     4021 ttccactctc aaacgcgtga atatttccgc cgcctggcga aagacgcttc acgttactct
     4081 tcaacgatct ccgaccctga caccaatgtg aagcaggtta aagtcctgca gctcattaac
     4141 gcataccgct tccgtggtca ccagcatgcg aatctcgatc cgctgggact gtggcagcaa
     4201 gataaagtgg ccgatctgga tccgtctttc cacgatctga ccgaagcaga cttccaggag
     4261 accttcaacg tcggttcatt tgccagcggc aaagaaacca tgaaactcgg cgagctgctg
     4321 gaagccctca agcaaaccta ctgcggcccg attggtgccg agtatatgca tattaccagc
     4381 accgaagaaa aacgctggat ccaacagcgt atcgagtctg gtcgcgcgac tttcaatagc
     4441 gaagagaaaa aacgcttctt aagcgaactg accgccgctg aaggccttga acgttacctc
     4501 ggtgcaaaat ttcctggtgc aaaacgcttc tcgctggaag gcggtgacgc gttaatcccg
     4561 atgcttaaag agatgatccg ccacgctggc aacagcggca cccgcgaagt ggttctcggg
     4621 atggcgcacc gtggtcgtct gaacgtgctg gtgaacgtgc tgggtaaaaa accgcaagac
     4681 ttgttcgacg agtttgccgg taaacataaa gaacacctcg gcacgggcga cgtgaaatac
     4741 cacatgggct tctcgtctga cttccagacc gatggcggcc tggtgcacct ggcgctggcg
     4801 tttaacccgt ctcaccttga gattgtcagc ccggtagtta tcggttctgt tcgtgcccgt
     4861 ctggacagac ttgatgagcc gagcagcaac aaagtgctgc caattaccat ccacggtgac
     4921 gccgcagtga ccgggcaggg cgtggttcag gaaaccctga acatgtcgaa agcgcgtggt
     4981 tatgaagttg gcggtacggt acgtatcgtt atcaacaacc aggttggctt caccacctct
     5041 aatccgctgg atgctcgttc tacaccgtac tgtactgata tcggtaagat ggttcaggcc
     5101 ccgattttcc acgttaacgc ggacgatccg gaagccgttg cctttgtgac ccgtctggcg
     5161 ctcgatttcc gtaacacctt taaacgtgat gtcttcatcg acctggtgtg ctaccgccgt
     5221 catggccata acgaagccga cgagccgagc gcaacccagc cgctgatgta tcagaaaatc
     5281 aaaaaacatc cgacgccgcg caaaatctac gctgacaagc tggagcagga aaaagtcgcg
     5341 acgctggaag atgccaccga gatggttaac ctgtaccgcg atgcgctgga tgctggcgat
     5401 tgcgtggtag cagagtggcg tccgatgaac atgcactctt tcacctggtc accgtacctc
     5461 aaccacgaat gggacgaaga gtacccgaac aaagttgaga tgaagcgcct gcaggaactg
     5521 gctaaacgca tcagcacggt gccggaagca gttgaaatgc agtctcgcgt tgccaagatt
     5581 tatggcgatc gccaggcaat ggcagccggt gagaaactgt tcgactgggg cggcgcggaa
     5641 aacctcgctt acgccacgct ggttgatgaa ggcattccgg ttcgcctgtc gggtgaagac
     5701 tccggtcgcg gtaccttctt ccaccgccac gcggtgatcc acaaccagtc taacggttcc
     5761 acttacacgc cgctgcaaca catccataac ggccagggcg cgttccgtgt ctgggactcc
     5821 gtactgtcag aagaagccgt gctggcgttt gaatacggtt atgccaccgc agaaccacgc
     5881 actctgacta tctgggaagc acagttcggt gacttcgcta acggtgctca ggtggttatc
     5941 gaccagttca tctcctctgg cgaacagaaa tggggccgga tgtgtggtct ggtgatgttg
     6001 ctgccgcacg gttacgaagg gcaggggccg gagcactcct ccgcgcgtct ggaacgttat
     6061 ctgcaacttt gcgctgagca aaacatgcag gtgtgcgtac cgtctacccc ggcacaggtt
     6121 taccacatgc tgcgtcgtca ggcgctgcgc gggatgcgtc gtccgctggt cgtgatgtcg
     6181 ccgaaatccc tgctgcgtca tccgctggcg gtttccagcc tcgaagaact ggcgaacggc
     6241 accttcctgc cagccatcgg tgaaatcgac gagcttgatc cgaagggcgt gaagcgcgta
     6301 gtgatgtgtt ctggtaaggt ttattacgac ctgctggaac agcgtcgtaa gaacaatcaa
     6361 cacgatgtcg ccattgtgcg tatcgagcaa ctctacccgt tcccgcataa agcgatgcag
     6421 gaagtgttgc agcagtttgc tcacgtcaag gattttgtct ggtgccagga agagccgctc
     6481 aaccagggcg catggtactg cagccagcat catttccgtg aagtgattcc gtttggggct
     6541 tctctgcgtt atgcaggccg cccggcctcc gcctctccgg cggtagggta tatgtccgtt
     6601 caccagaaac agcaacaaga tctggttaat gacgcgctga acgtcgaata aataaaggat
     6661 acacaatgag tagcgtagat attctggtcc ctgacctgcc tgaatccgta gccgatgcca
     6721 ccgtcgcaac ctggcataaa aaacccggcg acgcagtcgt acgtgatgaa gtgctggtag
     6781 aaatcgaaac tgacaaagtg gtactggaag taccggcatc agcagacggc attctggatg
     6841 cggttctgga agatgaaggt acaacggtaa cgtctcgtca gatccttggt cgcctgcgtg
     6901 aaggcaacag caccggtaaa gaaaccagcg ccaaatctga agagaaagcg tccactccgg
     6961 cgcaacgcca gcaggcgtct ctggaagagc aaaacaacga tgcgttaagc ccggcgatcc
     7021 gtcgcctgct ggctgaacac aacctcgacg ccagcgccat taaaggcacc ggtgtgggtg
     7081 gtcgtctgac tcgtgaagat gtggaaaaac atctggcgaa agccccggcg aaagagtctg
     7141 ctccggcagc ggctgctccg gcggcgcaac cggctctggc tgcacgtagc gaaaaacgtg
     7201 tgccgatgac tcgcctgcgt aagcgtgtgg cagagcgtct gcttgaagcg aaaaactcca
     7261 ccgctatgct gaccacgttc aacgaagtca acatgaagcc gattatggat ctgcgtaagc
     7321 agtacggtga agcgtttgaa aaacgtcacg gtattcgtct gggctttatg tccttctacg
     7381 tgaaagcggt ggttgaagcc ctgaaacgtt acccggaagt gaatgcttct atcgacggcg
     7441 atgatgtggt ttaccacaac tatttcgacg tcagcatggc ggtttctacg ccgcgcggcc
     7501 tggtgacgcc ggtactgcgt gatgtcgata ccctaggcat ggcagacatc gagaagaaaa
     7561 tcaaagagct ggcagttaaa ggccgtgacg gcaagctgac ggttgaagat ctgaccggtg
     7621 gtaacttcac catcaccaat ggtggtgtgt tcggttccct gatgtctacg ccgatcatca
     7681 acccgccgca gagcgcaatt ctgggtatgc acgctatcaa agatcgtccg atggcggtga
     7741 atggtcaggt tgagatcctg ccgatgatgt acctggcgct gtcctacgat caccgtctga
     7801 tcgatggtcg cgaatccgtg ggcttcctgg taacgatcaa agagttgctg gaagatccga
     7861 ctcgtctgct gctggacgtg tagtagttta agcttcacct gcactgtaga ccgacgtgga
     7921 tagggtgtcg gtctacggtt taaaagataa cgattactga aggatggaca gaacacatga
     7981 acttacatga atatcaggca aaacaacttt ttgcccgcta tggcttacca gcaccggtgg
     8041 gttatgcctg tactactccg cgcgaagcag aagaagccgc ttcaaaaatc ggtgccggtc
     8101 cgtgggtagt gaaatgtcag gttcacgctg gtggccgcgg taaagcgggc ggtgtgaaag
     8161 tggtaaacag caaagaagac atccgtgctt ttgcagaaaa ctggctgggc aagcgtctgg
     8221 taacgtatca aacagatgcc aatggccaac cggttaacca gattctggtt gaagcagcga
     8281 ccgatatcgc taaagagctg tatctcggtg ccgttgttga ccgtagttcc cgtcgtgtgg
     8341 tctttatggc ctccaccgaa ggcggcgtgg aaatcgaaaa agtggcggaa gaaactccgc
     8401 acctgatcca taaagttgcg cttgatccgc tgactggccc gatgccgtat cagggacgcg
     8461 agctggcgtt caaactgggt ctggaaggta aactggttca gcagttcacc aaaatcttca
     8521 tgggcctggc gaccattttc ctggagcgcg acctggcgct gatcgaaatc aacccgctgg
     8581 tcatcactaa acagggcgat ctgatttgcc tcgacggcaa actgggtgct gacggcaacg
     8641 cactgttccg ccagcctgat ctgcgcgaaa tgcgtgacca gtcacaggaa gatccgcgtg
     8701 aagcacaggc tgcacagtgg gaactgaact acgttgcgct ggacggtaac atcggttgta
     8761 tggttaacgg cgcaggtctg gcgatgggta cgatggacat cgttaaactg cacggcggcg
     8821 aaccggctaa cttcctggac gttggcggcg gcgcaaccaa agaacgtgta accgaagcat
     8881 tcaaaatcat cctctctgac gacaaagtga aagccgttct ggttaacatc ttcggcggta
     8941 tcgttcgttg cgacctgatc gctgacggta tcatcggcgc ggtagcggaa gtgggtgtta
     9001 acgtaccggt cgtggtacgt ctggaaggta acaacgccga actcggcgcg aagaaactgg
     9061 ctgacagcgg cctgaatatt attgcagcaa aaggtctgac ggatgcagct cagcaggttg
     9121 ttgccgcagt ggaggggaaa taatgtccat tttaattgat aaaaacacca aggttatctg
     9181 ccagggcttt accggtagcc aggggacttt ccactctgaa caagccattg catacggcac
     9241 taaaatggtt ggcggtgtaa ccccaggtaa aggcggcacc acccacctcg gcctgccggt
     9301 gttcaacacc gtgcgtgaag ctgttgctgc tactggcgct accgcttctg ttatctacgt
     9361 accagcaccg ttctgcaaag actccattct ggaagccatc gatgcaggca tcaaactgat
     9421 tatcaccatc actgaaggca tcccgacgct ggatatgctg actgtgaaag tgaaactgga
     9481 cgaagctggc gttcgtatga tcggcccgaa ctgcccaggc gttatcactc cgggtgaatg
     9541 caaaatcggt atccagccgg gccacattca caaaccgggt aaagtgggta tcgtttcccg
     9601 ttccggtaca ctgacctatg aagcggttaa acagaccacg gattacggtt tcggtcagtc
     9661 gacctgtgtc ggtatcggcg gtgacccgat ccctggctct aactttatcg acatcctcga
     9721 aatgttcgaa aaagatccgc agaccgaagc gatcgtgatg atcggtgaga tcggcggtag
     9781 cgctgaagaa gaagcggctg cgtacatcaa agagcacgtt accaagccag ttgtaggtta
     9841 catcgctggt gtgactgcgc cgaaaggcaa acgtatgggc cacgcgggtg ccatcattgc
     9901 cggtgggaaa gggactgcgg atgagaaatt cgctgctctg gaagccgcag gcgtgaaaac
     9961 cgttcgcagc ctggcggata tcggtgaagt actgaaaact gttctgaaat aaatatctgt
    10021 aataagaaat agccctcgcc gcttccctct acaggaatgg cgaagggctg tcggtttcga
    10081 catggttggc catcgtatga tggccttttt tatgccttaa atgttaacca cggtcgaaat
    10141 ctgaccgctg gcttgactgc atgaccacag ccactgtgga ttgccgcaac cgaccgcaag
    10201 ccacgaataa gaacgacggc gaatttcgaa tcgttacctg atgcactggc ttcccagcgt
    10261 ttgaccgtag cgttaagtaa atcaacaaaa gcgatgttca ccgcttatcg tcaagtcgtg
    10321 ccatttgtca gccaggatcg ggtctttacc ccggatattg cagcatcgca aaaattctta
    10381 gagggatatg ctataaatca aaaataaaat ctttgttgat tgtttatc
//
LOCUS       DACBVI010000153        10416 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.154, whole genome shotgun sequence.
ACCESSION   DACBVI010000153 DACBVI010000000
VERSION     DACBVI010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.154"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..813
                     /gene="tnpB"
                     /locus_tag="IU325_18840"
     CDS             <1..813
                     /gene="tnpB"
                     /locus_tag="IU325_18840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP5811063.1"
                     /translation="GWMRYRNSRQVTGVVKNVTVSQSCGKWYISIQTENEVSTPVHPS
                     ASMVGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQKCK
                     IQRLHSRIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAK
                     SGLNRTILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRCACCGHTAKENRLSQSK
                     FRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEIIQATA"
     gene            857..964
                     /locus_tag="IU325_18845"
                     /pseudo
     CDS             857..964
                     /locus_tag="IU325_18845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(948..1586)
                     /gene="ytfB"
                     /locus_tag="IU325_18850"
     CDS             complement(948..1586)
                     /gene="ytfB"
                     /locus_tag="IU325_18850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HAP5811064.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNALVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1804..2424
                     /locus_tag="IU325_18855"
     CDS             1804..2424
                     /locus_tag="IU325_18855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAP5811065.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2733..4145
                     /gene="cycA"
                     /locus_tag="IU325_18860"
                     /gene_synonym="dagA"
     CDS             2733..4145
                     /gene="cycA"
                     /locus_tag="IU325_18860"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HAP5811066.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPELSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(4190..4852)
                     /gene="ytfE"
                     /locus_tag="IU325_18865"
     CDS             complement(4190..4852)
                     /gene="ytfE"
                     /locus_tag="IU325_18865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HAP5811067.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQASKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(4960..5925)
                     /locus_tag="IU325_18870"
     CDS             complement(4960..5925)
                     /locus_tag="IU325_18870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HAP5811068.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFVSLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVVGVVIAVRAKPEKPLIEPVSES"
     gene            complement(6034..6894)
                     /gene="qorB"
                     /locus_tag="IU325_18875"
     CDS             complement(6034..6894)
                     /gene="qorB"
                     /locus_tag="IU325_18875"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313217.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAP5811069.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQSLTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADKSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKSVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            6983..7363
                     /locus_tag="IU325_18880"
     CDS             6983..7363
                     /locus_tag="IU325_18880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP5811070.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKMGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(7481..9424)
                     /gene="cpdB"
                     /locus_tag="IU325_18885"
     CDS             complement(7481..9424)
                     /gene="cpdB"
                     /locus_tag="IU325_18885"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HAP5811071.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRVGKIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            9614..10354
                     /gene="cysQ"
                     /locus_tag="IU325_18890"
     CDS             9614..10354
                     /gene="cysQ"
                     /locus_tag="IU325_18890"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HAP5811072.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPAFRVSIY"
BASE COUNT         2427 a         2665 c         2749 g         2575 t
ORIGIN      
        1 ggctggatgc gctaccggaa tagccgtcag gtcacgggtg ttgtgaaaaa tgtcacggtc
       61 agccagtcct gcggtaagtg gtacatcagt attcagactg aaaatgaagt atcaactccg
      121 gttcaccctt cagcatcaat ggtcgggctg gatgctggcg tggctaaact cgccacgctg
      181 tcagatggca cagtctttgg gcctgtaaac agttttcaga aaaaccagaa gacgctggcg
      241 agacttcagc ggcagttaag ccgcaaggtc aaattcagca acaactggca gaagcagaaa
      301 tgcaaaatac agcgactgca ttcccgtatc gcaaatatcc gcagggacta ccttcacaaa
      361 gtcacaacga ccgtcagcaa aaaccacgca atgatagtca ttgaggattt gaaggtcagc
      421 aacatgtcaa agtcagcagc gggtacggtc agccagccgg ggcgcaatgt ccgggcaaaa
      481 tcaggtttaa accgtacgat actggatcag ggctggtatg aaatgcgccg ccagcttgag
      541 tacaagcagc tctggagtgg cggtcaggtg cttgctgttc cgccagcgta cacaagccag
      601 cgttgcgcgt gctgtggtca tacagcgaaa gaaaatcgcc tgtcacaaag taaattcaga
      661 tgccaggtat gtggatatac agcgaacgcc gatgtaaacg gcgctcgtaa cattttagcg
      721 gcgggacacg ccgttcttgc ctgtggagag atggtgcagt caggccgctc gttgaagcag
      781 gaacccaccg aaataattca ggcgacagcc tgaacgtagc agggaaccac gtccttcagg
      841 gcgtggagga tgtcaacaca tcagtatggt ttcaccccag gcactggaag ccagacgtaa
      901 ggtcatcaaa gagacaatcc ttagctggct tttggttgac ccttcatcta ccgcgcacga
      961 ataaaactgc catccggctg gcgggtaaac aagacctgtt gattattccc cgtatcaatg
     1021 gttaagcccg tcaccacgcc gctggcgttc tggcggattt tcaccatctg cccgttttgc
     1081 aaattactca gcggcttacc cgcgccttca acctgcgcca ttgcatagac atcggtagcg
     1141 ggcagtccgt ggtcgcggaa cagctgcgcc atcgttttac cgggttcgac acggtaagag
     1201 cgccattgat tatcaattcc gctatctggc tggaatggtt gcgtttgggt ggtttgcggc
     1261 tgctcttctg gctggccttc ctgtattggt tctggagcga ccggggccac ctgatcagga
     1321 tcattttgtg gcgtaactag ctgcgcccgc agctgttctt ctgtcggtgg ttgtgattgc
     1381 gactgaatat ctaactgcgc ttcacgcgtc accaccagcg cgttgggcgt atcatcagat
     1441 ggaagcagaa atccgaccac caatacaatg gcggcaataa tgatcccccg acgatgcatc
     1501 ggcggcagcg ggtccatgaa gcgaaaattg tctggcgcgt gccagacttt ctccagggtt
     1561 ggttttagtt caaagcgccc gggcatggtt tcctcctgct ccgcgtcttg ttcctcaatc
     1621 atagcctatg aataagctaa cgctatgatg tccgtggtaa acccgccttt attatattca
     1681 tacgggatat tgctattgtt tctttttccc cgggatttgt catcattccc gcgacaaagt
     1741 tttacccaaa gaagtgtggc tgatatgctg cccgctactt tataccctaa gaaaggaaat
     1801 acgatgacca ccccaacttt tgacaccatc gaagcgcaag caagctacgg cattggtttg
     1861 caggtagggc aacaactgag tgaatctggc ctggaagggc tgctgccaga agcactggtt
     1921 gcaggcattg ctgatgcgct ggaaggcaaa catccggctg ttccggttga tgtggtgcac
     1981 cgcgcgctgc gtgaaatcca cgaacgcgcc gatgccgttc gtcgtcagcg tttccaggcg
     2041 atggctgctg aaggtgtgaa atacctggaa gaaaacgcca aaaaagaagg tgtaaatagc
     2101 accgaatctg gcctgcaatt ccgcgtgatc aaccagggcg aaggcgcaat tccggcacgt
     2161 accgaccgcg ttcgtgttca ttacaccgga aaactgatcg acggcaccgt gtttgacagc
     2221 tccgttgctc gcggcgagcc agctgaattc ccggttaacg gtgtgatccc tggctggatt
     2281 gaagcactga ctctgatgcc ggtaggttct aaatgggaac tgactatccc gcaggaactg
     2341 gcatatggcg agcgtggcgc aggcgcgtcc atcccaccgt tcagcaccct ggtgtttgaa
     2401 gtcgaactgc tggaaatcct ctaagcagcg cattctgttc ccctcgaacc agaggggagc
     2461 agacattcag caataaaccc ttcagtttgc caaacggcgc tattttgtgt tgcaaagacc
     2521 ccgtaagcgt gtatttttgt gagctgtttc gcgttatcac cgtgatatga cactcacttt
     2581 aaacataaaa ttaacattag atctaaatct tagtattcat cccgcgtatt gttacctaat
     2641 atcgatgagt cccgatacag attcgtcgta tcatagactg actaaaggcc gtagagcctg
     2701 aacaacacag acaggtacag gaagaaaaaa acatggtaga tcaggtaaaa gtcgttgccg
     2761 atgatcaggc tccggctgaa cagtcgctac ggcgcaatct cacaaaccga catattcagc
     2821 ttattgccat tggcggtgcc attggtacgg ggttgtttat ggggtccggc aaaacgatta
     2881 gccttgccgg gccgtcgatc attttcgttt atatgatcat tggttttatg ctctttttcg
     2941 tgatgcgggc aatgggggaa ttgctgcttt cgaatctgga atacaaatct tttagtgact
     3001 tcgcttccga tttactcggg ccgtgggcag gatatttcac cggctggacc tactggttct
     3061 gctgggttgt aaccggtatg gcagacgtgg tggcgatcac ggcttatgct cagttctggt
     3121 tccccgagct ctccgactgg gtcgcctcgc tggcggtgat agtgctgctg ctgacgctca
     3181 acctcgccac cgtgaaaatg ttcggtgaga tggagttctg gtttgcgatg atcaaaatcg
     3241 tcgccatcgt gtcgctgatt gtcgtcggcc tggtcatggt ggcgatgcac tttcagtcac
     3301 cgaccggtgt ggaagcgtca ttcgcgcatt tgtggaatga cggcggctgg ttcccgaaag
     3361 gtttaagtgg cttctttgcc ggattccaga tagcggtttt cgctttcgtg gggattgagt
     3421 tggtaggtac aacagctgcg gaaaccaaag atccagagaa atcactgcca cgcgcgatta
     3481 actccattcc gatccgtatc attatgttct acgtcttcgc gctgattgtg attatgtccg
     3541 tgacgccgtg gagttcggta gtcccggaga aaagcccgtt cgttgaactg ttcgtgttgg
     3601 taggtttgcc tgcggctgcc agcgtgatca actttgtggt gctgacctct gcggcgtctt
     3661 ccgctaacag cggtgtcttc tctaccagcc gtatgctgtt tggtctggcg caggaaggtg
     3721 tggcaccgaa agcgttcgct aaactctcta agcgcgcagt acccgcgaaa gggctgacct
     3781 tctcttgtat ctgtctgctc ggcggcgtgg tgatgttgta tgtgaatccc agcgtgattg
     3841 gcgcgttcac gatgattaca accgtttccg cgattctgtt tatgttcgtc tggacgatta
     3901 tcctttgctc gtacctggtg tatcgcaaac agcgtccgca cctgcatgag aaatcaatct
     3961 acaagatgcc gctcggcaag ctgatgtgct gggtatgtat ggcattcttt gtgttcgtgg
     4021 tcgtgctgct gacactggaa gatgataccc gccaggcgct gctggttacc ccgctgtggt
     4081 ttatcgctct ggggctgggc tggttgttta ttggtaagaa gcgggctgct gaactgcgga
     4141 aataaccgca ttatcatgct ggatggcgca atgccatcca gcttttagat cactcacccg
     4201 ccagggcgcg tgggaacagt acattgtttt ccagactgat gtgatccatc aggtcatcaa
     4261 tcagttcatt aataccgtta tacatcgctt tccaggtggt gcaggcttct ggcggcggtg
     4321 tgacgttatt ggtggtgtgt ttaatcactt ccagcagttc gcccgcttca tcgtgctcgc
     4381 tttccatcac gctgattggt cccattgcct ggctgcccat gccttgtttg atcatcggga
     4441 agagaatttg ctcttctttc atcatgtggc tggaaagctc ttcatgcagc atagtcaggt
     4501 attttgtcag cccttttggc acgctcggtt tgtcggcgtg aacgcgttcg actttgctgg
     4561 cttgcagaat cagctccggc agttgctcgc ggtgacgatc gtggtagcgc acgatgatat
     4621 ggtcgatgat ttctgccagc ggggcgctac gccagtcttt ctcaatcggt tgttcagcga
     4681 gctttgccag ctcagcttca atgacctcaa catccagctc tttacgtgcc gccgcacgcg
     4741 ccagcgtttg cttaccgccg cagcagtaat ccatatcata tttacgaaac agagccgaag
     4801 cgcgaggaat agagagcgcc agttcaccta aaggttggtc gcgataagcc atagctgata
     4861 cctcattctt aataagataa gatgcatttt aaatgcatct ttaaggcaaa agctataacc
     4921 cttacgtagc agaggcttat ttaactcact gccagcgtgt caactttcag agacaggttc
     4981 aattaagggt ttttccggtt ttgctctgac cgcaatgacc acgccaacca ctaacagcgc
     5041 gataccgctc agcgtcatta gtggcggcat ttgctggcgt aacaaaaagg tgtacagcaa
     5101 acctgccagc gtttcgaaga caatcagtgg cccgagaatc accgttggta atcgctggct
     5161 ggcgacgttc cagcaaagtg cgccgaccca tgagcaaagc acagctatcg caatcatcag
     5221 gctaacaaat acaagcggac gggggccaaa aggtagggag aagtccggcg tttgcgtatt
     5281 cagccagtaa caagcgacga gatagccgat aagcgaaacc ggcagcgtga ccagcgcctg
     5341 cgccgtcgcc cacatcatcg gatgtttgtc gggattttcc cgcagccagc gggcgttgcg
     5401 cagggcatac catgcccagc agaccacgga aactaacgcc agcacgatgc cagaggtata
     5461 acgtgcccag tcaaaatcgg ggagtccgtg gtttaactca gcaatattca cactcgccag
     5521 gccgatgcca atacaaatca gtgccggggc gagttttccc cacgcgagtt tgccgtcgcg
     5581 ctggctataa agcagattgg caaagacagg aatgaccacc ggcagggtgc cgataatcat
     5641 cgtggaaaca ggcgcgccag tacgttgaat ggcactggca aggcagaagt aataaatgag
     5701 gttgcccatc atagtgagca tcaaggcggt aagccagtcg cgacgcgcca actgacgcag
     5761 acgcacgcgc cccagccagg caatgggcag cgcaattaac cctaacgcca gataacgccc
     5821 catagactgc aacatcgccg ggtattccgg cacgatcaac ggcccgacaa aaataagccc
     5881 ccacatcaac cctgctaaca gggcgtacag cacgccgcta atcattactg gcatccattg
     5941 gtctgtcaga agaaacgttc aggaagcaga catgattgct cccggcatcc cgtcattatc
     6001 agagaggatg cgggaggatg ccactttaat taactagtta ttaacattaa aaagatggct
     6061 tacgctttcg gctaacgttg tcgttgggcg gccaatcaat ttgctaagcg ttttgctgtt
     6121 atcaaacaga ccacctttcg atgcgccaac gtcagaatcc gccagcatat ccgccagtcc
     6181 gtcgggcagt ccgacgcttt tcagtgccgc ggcgaaatcg gcttcgctca gattttgata
     6241 ggtaacggat tttccgctct gtttggtcag ctctgccgct aactgtgtca acgtccaggc
     6301 actatcgccc gccagttcgt aaaccttgcc ttcgtgaccg gcttcgctaa tcacgcgtgc
     6361 cgcagctgcc gcataatctg cccgcgttgc tgaggcgatt ttgccatcgc ccgccgcacc
     6421 gataaatacg ccgtgttcca gtgctgccgg ggcgctggcg aggtagtttt cggtgtacca
     6481 gccgttgcgc agcagggtgt aaacgatgcc agaatcagcc aacattttct ccgtctcaat
     6541 gtgctcatcg gcgaggccga gcggagattt atccgcgtga agcagactgg tataagcgat
     6601 aaatttcaca ccagccgcct ttgcggcatt aataacatta cgatgctgcg gggcacgttg
     6661 acccacttcg ctggaagaga tcagcagtag tttttccact ccctgaagtg cagatgtcag
     6721 tgcggcttca tcgccgtagt cagcctgacg cacggtaatg ccttgtgctg tcagtgactg
     6781 ggcttttgcc ggattacgaa cgatagccac tatttggctg gcaggaaccg ttttcatcaa
     6841 ggattcaata acatagtgac caagttggcc agtggcacca gtaattgcga tcataggaag
     6901 tctccatcgt tttgtcttgt gtttaaatac gctaacacct aaacttactt ttagtaagta
     6961 cgtacaaaaa ggtaagtatg agatgagtca ggttagcctg tcgcaacaac tgaaagaggg
     7021 aaatctcttc gctgaacagt gtccgtcgcg cgaggttttg aaacacgtca ccagccgttg
     7081 gggggtgttg attctggtgg cgctacgcga aggtactcat cgctttagcg acctgcggcg
     7141 caaaatgggc ggggtgagtg aaaagatgct tgcgcagtcg ttacaggcgc tagagcagga
     7201 tggttttctt aaccgtatcg cgtatccggt ggtgccgccg catgtggaat atagcctcac
     7261 gccgctgggc gagcaggtga gcgaaaaggt tgctgcactg gcggactgga ttgagttgaa
     7321 tttgcccgag gtgttggcgg tgcgggatga acgtgcggcg taactgcagg atgcgcggcc
     7381 tacggtgtat gacttgtagg gcggataaga tgcgccagca tcgcatccgc caataaatgc
     7441 cggatgcggt tgatgatttg caggccgaaa aagaagtgta ttacttactc aaatccacct
     7501 gataaatcgc aaacccgata tcatcggtcg cgactttatt catcggatat tgccctttct
     7561 ctttaataaa cgccgcggct ttatctgatg gagaggtttc gaaacggata tccagtttct
     7621 tatcgccagc tattggtgct aaacgccagt tgttatctgc cgccgggtga attttcccca
     7681 cacgtttcga ctcatcagca atccacgctg ccagcaccga gcggttctca tccggtgaag
     7741 caaaagcgat atggctgtcg cccgtaccgg caaatttgcc gccgtaagcg cgatagttat
     7801 tggtggcaac gaggaacatg gcgttcggat caatcggttt gccattaaag gtcaggttct
     7861 taatcctttc cgcattggca ttaatcatct ggcactcgcc gtcataacgg gcgggttggg
     7921 taacatcaat ctgataattc acaccatcaa taacatcaaa gttataggtg cggaaaccat
     7981 cccagttgat gagtgactgt ggtttcgtgc tgttaggatc aatctggtta aactgtccgg
     8041 cggagcactc cagccactct ttcacctctt taccgctggc tttcaccaca atcagcgtat
     8101 tggggtagag ataaagatcg gcggcattac ggaaggtcaa ctggcctttt tccacctcca
     8161 catagcttgc cgggtcattt ttgcgaccac caactttaaa cggtgcggca gctgaaagca
     8221 ccggcagttt tgccagatcc ggatcgccct gaatgtaatg ctcgacatac gctttttgcg
     8281 cgttgttcac tacctgcacg gtcggatcgt cttgcactag cgccagatag ctatacatat
     8341 tgtcggcgga tttaccgatt ggcttgctga cgaactggcg tgtggcatcg tgatcggctt
     8401 tgagtgtttc taccagcttg ctgtcttccg ccgcgaggga ttttttattc gcgatgtcgt
     8461 aaatcggccg tgcttccgct ttcgcctgcg tcacctgcca tttaccgctg tcattactaa
     8521 gttgtaagtc gaccacacca agatgatcgc cccacatgcc tggcattacc gccggaacac
     8581 cattcagcgt gcctttcgcg atatcagccc cttcgatatc agcaaaatct ttacctggga
     8641 aaacggcgtg agcatggcca aacataatgg cgttaacgcc cggaatttca ctgaggtaat
     8701 aaactgagtt ttctgccatc actttatacg gatcggcaga cagaccagaa tgcgccagaa
     8761 cgacaacgac atcggcacct ttctcgcgca tttcaggcac gtacttgcgc acggtttcgg
     8821 taatatcatt caccgtcact ttcccggata aattagcttt atcccagccc atgatttgcg
     8881 gcggcacgac gccaatatag ccaatcttca gcgtctgttt ttttccgtct ttatcgacca
     8941 cttcggtgtc tttaattaaa tacggtgtaa acattggctg tttggttctg gcgtcaatga
     9001 cgttggcatt tacataaggg aatttcgctc ccgccagcgc atttttcagg taatccagac
     9061 cgtagttaaa ctcgtggttg ccaagcgtgc cgacggtata gtccagcgta tttaatgcct
     9121 tatagaccgg gtgaatatca cctgctttta aacctttcgc cgacatgtaa tcggccagcg
     9181 gactcccctg aatcaaatcg ccgttatcga ccagtacgct gtttttcact tcattgcggg
     9241 catcgttaat caggcttgcg gtacgtacca gtccgaattt ttccgtggcg gtgtctttgt
     9301 aataatcgaa atccatcatg ttgctatgca gatcagtggt ttccatgata cgcagatcga
     9361 ccgtcgctgc attcacactg gcggcaatca gcgtggccag gagcgttgcg ctaaacttaa
     9421 tcatcaggga catcctttta tcatcgggaa tacgaaagaa aagggagaat aaacgtctta
     9481 cttatagaac agtgaagaat gccacaattt tacgctttga aaatgatgac actatcacag
     9541 ttggcgcatt cattaacgat agggtataag taaaacaata agttaacacc gctcacagag
     9601 acgaggtgga gaaatgttag atcaagtatg ccagcttgca cggaatgcag gcgatgccat
     9661 tatgcaggtc tacgacggga cgaaaccgat ggacgtcgtc agcaaagcgg acaattctcc
     9721 ggtaacggca gcggatattg ccgcacacac cgttatcatg gacggtttac gtacgctgac
     9781 accggatatt ccggtccttt ctgaagaaga tcctcccggt tgggaagtcc gtcagcactg
     9841 gcagcgttac tggctggtag atccgctgga tggtactaaa gagtttatta aacgtaatgg
     9901 cgaatttacc gttaacattg cgctcatcga ccacggcaaa ccgattttag gtgtggtgta
     9961 tgcgccggta atgaacgtaa tgtacagcgc ggcagaaggc aaagcgtgga aagaagagtg
    10021 cggcgtgcgc aagcagattc aggtccgtga tgcgcgcccg ccgctggtgg ttatcagccg
    10081 ttcccatgca gatgcggagc tgaaagagta tctgcaacag cttggcgaac atcagaccac
    10141 gtccatcggc tcttcgctga aattctgcct ggtggcggaa gggcaggcgc agctgtaccc
    10201 gcgcttcgga ccaacgaata tttgggacac cgccgctgga catgctgtgg ccgcagctgc
    10261 cggagcgcac gttcacgact ggcagggtaa accgctggat tacactccgc gcgagtcgtt
    10321 cctgaatccg gcgttcagag tgtctattta ctaaattcag atggcagaaa cagtgtattt
    10381 cctgattctg ccatcctgat ttctcccaac ctaaaa
//
LOCUS       DACBVI010000154        10355 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.155, whole genome shotgun sequence.
ACCESSION   DACBVI010000154 DACBVI010000000
VERSION     DACBVI010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.155"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            117..413
                     /locus_tag="IU325_18895"
     CDS             117..413
                     /locus_tag="IU325_18895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HAP5811073.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            415..711
                     /locus_tag="IU325_18900"
     CDS             415..711
                     /locus_tag="IU325_18900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP5811074.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            814..2175
                     /locus_tag="IU325_18905"
     CDS             814..2175
                     /locus_tag="IU325_18905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HAP5811075.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(2448..2666)
                     /gene="ogrK"
                     /locus_tag="IU325_18910"
     CDS             complement(2448..2666)
                     /gene="ogrK"
                     /locus_tag="IU325_18910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HAP5811076.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(2748..3911)
                     /locus_tag="IU325_18915"
     CDS             complement(2748..3911)
                     /locus_tag="IU325_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HAP5811077.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPIRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDTTLGAIVEAIATRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQITITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVRQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVKVSGFKRVIDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            complement(3911..4390)
                     /locus_tag="IU325_18920"
     CDS             complement(3911..4390)
                     /locus_tag="IU325_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP5811078.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAYQFLG
                     VGEENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISRVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMATKFTGMTGAG"
     gene            complement(4405..6852)
                     /locus_tag="IU325_18925"
     CDS             complement(4405..6852)
                     /locus_tag="IU325_18925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP5811079.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRTASKSLSGDIRETQKSLRE
                     LNGHASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQVKYNRLTDSVKRQQRELASVGINTRNLAHDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTMAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSAEMAALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPVFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTAASTGKLDKLTAAFKASDGKTAELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRNLTQTATKYVLKLDGWIKKNKSLASTIGLIVGGALALTGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFTNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTQAATYGSG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            complement(6845..6964)
                     /locus_tag="IU325_18930"
     CDS             complement(6845..6964)
                     /locus_tag="IU325_18930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HAP5811080.1"
                     /translation="MADVAVIFHWPPSELYPMSLTELITWREKALRRSGNTNE"
     gene            complement(6997..7272)
                     /locus_tag="IU325_18935"
     CDS             complement(6997..7272)
                     /locus_tag="IU325_18935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HAP5811081.1"
                     /translation="MNKENVITLGNPVKRGEQVIEQVTLMKPNAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            complement(7329..7847)
                     /locus_tag="IU325_18940"
     CDS             complement(7329..7847)
                     /locus_tag="IU325_18940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HAP5811082.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVACTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            complement(7860..9050)
                     /locus_tag="IU325_18945"
     CDS             complement(7860..9050)
                     /locus_tag="IU325_18945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP5811083.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGEDEETKLAQTV
                     SNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFG
                     YISAWGCKTISEVKAYRQNFSQRELMVIWPDFLAWDTVTSTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGN
                     RTCSDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKT
                     NGYIVDATCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITDKYLANLVTS
                     VNSN"
     gene            complement(9110..9703)
                     /locus_tag="IU325_18950"
     CDS             complement(9110..9703)
                     /locus_tag="IU325_18950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP5811084.1"
                     /translation="MRIGYVRVSTNDQNTELQRNALECAGCELIFEDKISGTKSDRPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVILVEELRERGINFRSLTDAIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLEAARAKGRIGGRRPKLTASEWEQAGRLLAAGES
                     RQRVALIFDIGLSTLYKKFPASVTKNKLCQPLANRDK"
     gene            9901..10218
                     /locus_tag="IU325_18955"
     CDS             9901..10218
                     /locus_tag="IU325_18955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811085.1"
                     /translation="MNIDTNKKTTTLKYFVEAYPERITVQAWERLVNEIGDLKEVYGY
                     IAFLHDDGYLTGKVIFDGSPESIDGWSVDTASLRITSQGYHYWKKLNPTTQGSSGILR
                     RNR"
BASE COUNT         2339 a         2865 c         2586 g         2565 t
ORIGIN      
        1 agtgggcact gacgagcact cgttgtgctt tctttgttta ccatcccata cttatcctcc
       61 tgaacatgtt catgtgccaa aatatcttct aatactttcg tgacgctatc cggtttatga
      121 catacactga tgtaagattt ttacttacag gcgggcgtaa aatggccaga acgatgacgg
      181 ttgatctcgg cgatgagttg cgtgaattta tcgagtcctt aattgaatcc ggcgactatc
      241 gcacccaaag cgaagtgatc cgtgagtcac tgcgattact tcgagaaaag caagcagagt
      301 ctcgcctgca agcgttacgt gacatgctcg cagaagggtt gagtagtggt gaagctcagc
      361 cgtgggaaaa ggacgcgttc ttacggaagg tgaaagcagg gataaggaaa taatgtgcgt
      421 attattaaac tcatgccaaa agccaatgag gatttagaag gcatctggta ttacagctat
      481 caccatttcg gcgaaccaca agccgacaga tatgtggaac atctttcaga cgttctccag
      541 attctgagta acaataatat aggtacgccc agacctgaat taggcgaagg tatttttgtt
      601 cttccttttg aacgccacgt catttatttc ctgcaatcac caggggaaat tatcgtcatt
      661 cgtatcctta accaaaacca ggacgccacc agacatttac actggagcta aacccgccga
      721 ttgcgctaca atgcccgccc ttaaagtggg ggcactcccc taaccgcttc atcaggtgaa
      781 gcggatctga cctgtcatca gaacgagaga attatgttta aaccggaact cctttccccg
      841 gcgggaacgc tgaaaaatat gcgttacgct ttcgcttatg gcgcagatgc tgtttatgcg
      901 ggccagccgc gttattccct gcgtgtgcgc aacaacgaat tcaaccacga aaatcttcag
      961 ctcggcatca atgaagccca cgcgctgggg aaaaagtttt atgtcgtggt caacattgca
     1021 ccgcacaacg ccaagctgaa aacctttatc cgtgacctga aaccggtggt ggaaatgggg
     1081 ccggatgcgc tgattatgtc cgatccaggg ctgattatgc tggtgcgtga gcacttccct
     1141 gaaatgccga tccacctttc ggtgcaggct aacgccgtga actgggcgac ggtgaaattc
     1201 tggcagcaaa tgggcctgac ccgcgtgatc ctctctcgcg agctgtcgct ggaagagatt
     1261 gaagagatcc gcaatcaggt gccggatatg gagatcgaga tcttcgttca cggcgcgctg
     1321 tgcatggcct actccggtcg ctgcctgctc tctggctata tcaacaagcg cgacccgaac
     1381 cagggcacct gcaccaacgc ctgccgctgg gagtacaacg tccaggaagg gaaagaagat
     1441 gatgttggca acatcgtaca caagtacgag ccgattccgg tgcaaaatgt tgagccgacg
     1501 ctgggtatcg gcgcaccaac cgacaaagtg tttatgatcg aagaggccca gcgtccgggc
     1561 gagtatatga ccgcgtttga agatgagcac ggcacttaca tcatgaactc gaaagatctg
     1621 cgcgccatcg cccatgtaga acgcctgacc aaaatgggcg tgcattcgct gaaaatcgaa
     1681 ggtcgtacca aatctttcta ctattgtgca cgcaccgcac aggtttaccg caaagctatc
     1741 gatgacgccg ctgcgggaaa accgttcgat accagcctgc tggaaactct ggaaggtctg
     1801 gcgcatcgtg gctataccga aggtttcctg cgtcgtcata ctcacgacga ttatcagaac
     1861 tacgaatacg gttattcagt ttctgaccgc cagcagtttg ttggtgagtt taccggtgag
     1921 cgcaaggggg acctcgcggc ggtagcggtg aaaaataaat tctccgttgg cgacagcctt
     1981 gagctgatga cgccgcaagg caacattaat tttacccttg agcacatgga aaacgccaaa
     2041 ggcgaagcta tgccgatagc accaggcgat ggttatactg tgtggctccc ggtcccgcag
     2101 gatcttgagc taaattacgc gctgctgatg cgtaatttct ccggggaaac cacgcgtaac
     2161 ccccacggta agtgattaat ttcgattatt tttcccggat ggaaaattct tagaaaccga
     2221 tcacatacag ctgcatttat taaggttatc atctgtttcg ctgaaaaaca taacccataa
     2281 aatgctagct gtaccaggaa ccacctcctt agcctgtgta atctccctta cacgggctta
     2341 ttttttacgt acaacaaatt gaaataaaag gatttatttc tggtcacgtc cacacattga
     2401 ccacatcgac aaaaaagccc ctcgactgag gggctttctg tttgtaatta catccacata
     2461 atttgctgcc ctgacggcaa cgggtgcggc ctcacggcgt ggacttctcc cggcttcaca
     2521 atgtatcgct gtaccgactc ataagtgatg aacgtggcgc tgcaattcac gttctgacac
     2581 tggtgataac gctcttttgt cgtgtcagtg atatagcggc ttgtacgcgc atgtgcggca
     2641 tgctggcata aaggacaatg aaacatcgcg agcacctctt ccggttttgt tgatggtgcc
     2701 attttagtta atttatccct ataaaacaaa cagataaata aaacacatca ctcatcatct
     2761 tcggtttcgt actccacatc agaaagcctg acctcaagct ccaaggacgt cgtgaagccg
     2821 ctattattca gaaaatgtgt caccttagtg attgtccagt cctgctcgtc tatgacgcgc
     2881 ttaaagcctg acactttaac cggtgtttcc gtgtaaatat ctgcccgacc ggtagccagg
     2941 ctgatggaga actccgccac accccgttgc agtttatccc acttcgcctg agcggcgcgc
     3001 atggcctgcg ctttcgtggc atataccgtg gtcagggcaa aaacgttgtc agcctcaccg
     3061 gccatgtatt caccttcgcg cgcttccggt actttaggcg ctttcttctg cctgaccggt
     3121 ttcgctttcg ggtgctccag tgcgcgcagg tgtttctctt tctttttgcg tttcagtttt
     3181 accttctgct tttgcggctt cgggtctttg gtgtgtaacc actttgccgt tacgccggtg
     3241 taggctccac ggtcagcaat cgcaaaatga tgacggtcgc cgtcgctacg ggtgatggta
     3301 atctgcggga tttttttacc gctggccgtc accccctgcc ccgctttgag aaacagcagt
     3361 tttcccattt ttaccgacac ctcgccgccg ttgcgttcag caagacgggt caggaatttc
     3421 gcatcagact cctgcgactg gtcgatgtgc gggattttaa ttccggccag tgacggcgcg
     3481 acactggctt ccagcctgtt acgggtggct atcgcctcaa caatcgcacc gagcgtggtg
     3541 tcatgccagg agccttcccg gcgggaattg agcgtcccgc gaaaatctgc actccgggcg
     3601 cggatggtaa ccacatccgg cgcgccccgg tgttcaacct catcaacggt aaatttccct
     3661 ttgcatacca gggcaaaacc tttccagccg atatacaccg tcaggacagc gccacgaatc
     3721 ggtagcccga cctgcccgtc ggcatcgttc agttcaatat caagctggtc agcctcaaag
     3781 ccccggttat ccgtcagggt catgctcatc agacggtcgc tgatattgcc ggtaatatcc
     3841 ctgctgtcga gcatcagcat gtaatccggc gtcagcgtac tgcctgcatc aaatgtcagc
     3901 gcatccagca ttatcccgcc cccgtcatac ccgtgaattt agtcgccata ctgccagcct
     3961 taccgatgag cgattctgcc tgtttaccga tatcgccata aagcgcggcc agtgattcat
     4021 caacgcgggt gagcgacagc gtaaaatcaa ttttccgggg tgtgccgtct gcaaagaaaa
     4081 tactccctgt ttcactcacc ctgctgatga catacatgcc gtaaatcatg ccggtgccat
     4141 ccagcaacgg ccacgcccgg ccttcctctg ccatcagtct gagcgtggtc atcgtcagct
     4201 ttccgccggt cagttcagga taaagcacac cggcaagcgt gatgttttcc tcacccacac
     4261 cgagaaactg gtaggcatcc cgtttaccga tacgggaatt tgacggccag cgataatctg
     4321 attcacgctg catggtctgg tgtggcagcg tctggcgcat aaaaacaaac atacctaacg
     4381 cgagcatcat ttttcgtcac ctccttaacc gtcatgcatc atgctggcac gggcgcgcgc
     4441 acgtttatcc cgctcgtatt tttcgagcgc atcctgtaac tggcggtcaa gctgtgtccc
     4501 cggcgcagta ccacccgtca ggctgatgtg atattcgttt ttactctggt ccacataaga
     4561 gcggcctgcc ggtgccgtga ccggctgata agcctgatag cctgcataag agctggtcgc
     4621 cggaatataa ccaccgctgc catacgtggc ggcttgagtt ctggcggcgg tctggtcaag
     4681 tgtgtctgac tctttgttga tgaccccgag tttttccagt acccagtcaa taccgctgcg
     4741 cagtttgttg aacgcattaa gcggcagcat cagcgcgtca gccagtgcct gcccgaacat
     4801 gacgcccgtg tcacggcaac ggttcagggt gtcctgggtg gctttaaccg gagcaatcag
     4861 gtttgtaaac cactgccacg cggcctgtaa cttttcgccc agccagtcaa acaccggttt
     4921 cagtggcgtg aacagttccc ccaccggcgc aaatgccgct ttcagccctt caaccacacc
     4981 gccaaagaat gcgctgacag gctcccagta tttacggata agtaacgccc cggcgacaat
     5041 agcggccacc acggccacaa ccggccagct aatcgccccg atggccgtca taacggcact
     5101 gccaaccgtc gtgaagattg cccccattgc gcctgctgcc gcgatgatgg cattgatgcc
     5161 ggtgataacc ggccaggcga caagaccaat ggcaccgatg atgccagtaa gcgccagtgc
     5221 gccaccgaca atgaggccga tggttgacgc cagtgatttg tttttcttga tccagccgtc
     5281 gagttttaac acatactttg tggccgtctg cgtgagatta cgcagtgcgc cttcctgctg
     5341 gtcaaacagg tcagtcccca ccgcctcata agcagactga aactccttaa agtcaccgcc
     5401 gaggttatcc tgcatgatat ttaccagctc ggcggtcttc ccgtctgagg ctttaaacgc
     5461 agcggtcagt ttgtccagct ttccggttga ggcggcagtc atcagcacgg cggcggctga
     5521 gctggcttcc tccccgaaaa tggttttcat gtattcagcc tgctgggcag taccgagccg
     5581 gtttttctca aaactggcct gcatttcttt cagaatggta aatactggtc gggtgtttcc
     5641 cttactgtct gaggttttca cgccaagctc tttgagtgca tcccatgctt ttcccgtcgg
     5701 tgcctgcagg cgacttaaca cggcacggct tcccgtcccc gccattgagc ctgtgatttt
     5761 tgcatcatgc agcgctccga ccattgcggc ggtttcttca atgctgacac cggcattttt
     5821 tgccacaggt gcagcatagg tcagcgcatc gctcataccg tcaaaatcgg cggctgtttt
     5881 gttcatcgtc atggagagaa catccccgat atgagcgacc ttatcgtttg aaagctgaaa
     5941 ggcggatttc atccccatca gcagggcagc gttttcttcc atcgtgcgac ggttcgccag
     6001 cgccatattc agcgtgaccg gcgttgccgc ctgaatggca tcaacatccc caccggcttt
     6061 cgcaataata atctgcgcac ctgctgcatc atctgccgag gcggcggtat tgtcgccaag
     6121 ctgacgcgcc tgtttgcgta atgcggccat ttcggcggag tcttttgcca ctccgagcac
     6181 agcctgtaat tctgagtttt tctgcgcaaa ttcataaccg ggcatcagca gcttaactcc
     6241 ggccatcgtt cccgccgccg caatccccac accggcagcg cctactgagg ccatatttcc
     6301 ggccagttcc tttccggcct gataacgctg ttttactgcg ttaagttttg cctgttgtgc
     6361 actgacacgc gccagtgcgt cacgctgacg gttaagctgt gcggtggttt cactgatacg
     6421 gtttttcagt ccctgctcat catgtgcaag attgcgggta ttaattccca cagaggccag
     6481 ttcccgctgc tggcgtttaa ccgaatccgt caggcggtta tatttcacct gtaagtcctc
     6541 cgccgcacgc tttgcggatt ccagcacttt cgcctgagca cgggtcggac gttcggtatt
     6601 tttaaactgt gtggcaaggg cttcggcctc ctgccgtgcc ttttcaagtg catgaccagt
     6661 cacggcgagc tgtgcgctgg tcttgcgaaa tccctcaata cgggatgcgt gaccgttcag
     6721 ctcgcgcagt gatttttgtg tttcccggat atcccccgac agcgacttgc tcgctgtacg
     6781 gatggattta aacgggcggg atgcctggtc aacagccctg agcaatacct gtaattttac
     6841 attgttactc attcgtgttt ccgcttcgcc ggagcgcctt ttcgcgccat gtgatgagtt
     6901 cggtcaggct catgggatac agttctgatg gcggccagtg aaatatcact gccacatccg
     6961 ccatcaggtc atcgaccgag agatttttcg ggaacgtcac tgcaccgagt tcggcgacaa
     7021 aaaaccgacc accttaccgg ccagcgccac aaggtcaggc agttccagtg cggcgacttc
     7081 ctgctcggtc agcatcggtg ccgtcatgcg cggcagcacc ttaatcagtg catcgacttc
     7141 ggagttcgcg accgcagcca gactgacacc gcgcagcgtc ccggcattgg gtttcatcag
     7201 cgtgacctgt tcgatgacct gctcaccacg cttgaccgga ttgcccaggg taatcacatt
     7261 ttctttgttc atggttttct cacttctgaa tcagggttaa ccggtcagcc tggctgaccg
     7321 gatgaaaatc acaggccgat attacggcgg tgttgctcca gccggtcgac gccgttcacc
     7381 ttctcaatca tgttgatggt gtcgatttcg accagctcct taccgtccat cgtcagccgg
     7441 aaataggtgc aggccacgga gattttcgac tcggtgtctt ctccctgttt accctcgccg
     7501 gtgtcgattt ctttctgacg gccacgcatg accacttcga cggccaccgt ttcgccggta
     7561 tcgtcgcgct ggtaagagcc tgcaaaacga atcggtacgg catccacacc ggttgcggcg
     7621 taaagctccc agataaccga atccgggaag cccccgagcg accactccat tgacagcgca
     7681 tcgtcatcaa ggccgaggtc taccggtgcg ctgccattca tccccgcacc gcgatagttt
     7741 tcgagcttac gggtcagttt tggcagcgtg acggactttg caacgccctg atagctgtag
     7801 ccgttcagaa agacgttcat taacttgagt ttgcgcggca ttgccatcgg tcaggctcct
     7861 taattgctgt taaccgaagt gaccagattt gccaggtatt tatcggtaat acgctggcgc
     7921 agggtcaggt tttcaagagg aggcaccggt gtatagtcgt agtcgatata cagttttccg
     7981 gccttgaggg tttccgcatc gttggattct tcgctgaacc agcaggtcgc atccacgata
     8041 tagccgtttg ttttcagctc acggaatttg gcattgatgc cgtcaacgat gtcgcgaatc
     8101 agcgttgcgg tgatgggctt gtccaccgcc cacatgtgcg cctcagccat cgtgtcggcc
     8161 agcacctgcg cggtgcgggt gtagttttca aagagaaaca gtgggtcatc agagcaggta
     8221 cggttacccc agaatcggaa accgtcgcgg cgaatcagcg tagtgacgcc tgactcgtta
     8281 agcaggtcag catcggtgcc ggactcctgc aaatcccaga atacagatgc gctgatgccg
     8341 gtaacaccgt tcaccccgac attggacagc gttttatgcc agccctgctc ctggtcgatt
     8401 ttggcacgca gccccagtgc acgggcggtg gcatacgcgg tggcggtggt actggtgacc
     8461 gtatcccatg cgaggaaatc cggccagatg accatcagct cacgctggct gaaattctgg
     8521 cggtaggctt tcacctcgga aatagtctta cagccccacg cgctgatata tccgaaagcg
     8581 cgcagcttct gacagacgga tgccagtgca acagccacct ctttggtatc cagtcccggc
     8641 acaccgagaa tacgcggttt aacaccggtt accgactccg ccgccagcag ggctttcagt
     8701 ccggtgtact gaccgttttc gtcggtggtg ccaatgatat tggaaacggt ctgcgcaagt
     8761 ttcgtttcct cgtcttcgcc ggtgccgtct tccacacgca cgacaacggt gaccggtttt
     8821 gactggtcag cgatggcctg caacgatgct gccagcgtgc cttttttacc ggcctttgca
     8881 attgcgctct gcacattggt aatcagcacc ggtttattga gggggaaggt ttccgcatcc
     8941 gcatcgctgg ccgtgcagac catgccgaca atggcagtgg atacggtgga aatgacgcgg
     9001 gtgccgtcgt taatctccag cacctgcacg ccgtgatgat agtcactcat ccgtttaact
     9061 ccgtggttaa tgggtgagtg atattttcag ttgtgccgga gatgtcaggc tatttgtccc
     9121 ggttggctaa gggctgacac aatttatttt ttgtcactga ggcggggaat tttttataga
     9181 gcgtggacag gccaatatca aaaatcagtg ccacgcgttg acgtgattcc cctgcagcca
     9241 gcaaccgccc ggcctgctcc cactcactcg cggtgagttt cggacgtctg ccaccaatac
     9301 gacctttggc tctggccgcc tccagtccgg cgcgtgtccg ctcgacaatg agttctcgtt
     9361 ccatttcagc cagggcaccc atcacatgaa agaaaaaacg ccccatcggc gtgctggtat
     9421 caatagcatc cgtcaggctg cgaaaattaa tgccgcgttc gcgcagttcc tcaaccagaa
     9481 tgaccagatg ccgcatacta cgccccagcc tgtccagctt ccagactacc agagtgtcac
     9541 ctgctgataa tgttctgagc agttttttta gtcccggtcg gtcggacttc gtgccgctga
     9601 ttttatcctc aaaaatcagc tcacatcctg cgcactccag cgcgttacgc tgcaattccg
     9661 tgttctggtc atttgttgat acacgaacat aacctatacg cataaaaatc cctataaaaa
     9721 cagaataatg acacagaatt atctatatat gtacaagcgg acaaggatat gggcactcag
     9781 ttacagtaat cttgctggat aagaatattt actgatagtg gatacatagc ccacttagca
     9841 ttcccatgtg aaacaattaa gtatggtaag ataggttgac ctcaataaaa ggaaaatctg
     9901 atgaacatcg ataccaataa aaaaaccacc acactgaaat attttgtcga agcctatcca
     9961 gagagaatca ctgttcaagc gtgggaacgc ctagtcaatg aaattggtga tttaaaagaa
    10021 gtttatggat atatcgcctt tctgcatgac gatgggtatc ttacaggaaa ggtaatcttc
    10081 gatggttctc ctgaaagtat tgatggatgg agtgtagata ctgcctctct aaggattaca
    10141 tcacaaggtt atcattattg gaaaaaatta aaccctacaa cacaagggag tagcggaatc
    10201 ctcagaagga acagataact aataaagatg gtagcaccat ttggtgttac catcttaaaa
    10261 tataccgtta caatcagcac tttccatttt tatctaacaa tcaatactag gacattccct
    10321 tctttcaaca tcatatttaa ccgattccat aaaac
//
LOCUS       DACBVI010000155        10315 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.156, whole genome shotgun sequence.
ACCESSION   DACBVI010000155 DACBVI010000000
VERSION     DACBVI010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.156"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            6..1688
                     /locus_tag="IU325_18960"
     CDS             6..1688
                     /locus_tag="IU325_18960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP5811086.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTYSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1740..2897
                     /locus_tag="IU325_18965"
     CDS             1740..2897
                     /locus_tag="IU325_18965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HAP5811087.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2863..2985)
                     /locus_tag="IU325_18970"
     CDS             complement(2863..2985)
                     /locus_tag="IU325_18970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811088.1"
                     /translation="MTKHPTGIYVGCFVKVIRRRLRLEQRRSVINYSPFVLQHP"
     gene            3189..4874
                     /locus_tag="IU325_18975"
                     /pseudo
     CDS             3189..4874
                     /locus_tag="IU325_18975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            4912..7284
                     /locus_tag="IU325_18980"
     CDS             4912..7284
                     /locus_tag="IU325_18980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAP5811089.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTAVS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPESDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDISGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KYAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNRVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLTWQEARKARIILGKTNAQYISEYSDYKQYVDERLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7329..10124
                     /locus_tag="IU325_18985"
     CDS             7329..10124
                     /locus_tag="IU325_18985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP5811090.1"
                     /translation="MEIIMRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGL
                     RYMIYPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETF
                     ESMGLRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAE
                     RGVITEEWRAHQDAKWRTSQARRPFLQANTRNLDREPIGLMDTVATVTPAQLRQFYQR
                     WYQPNNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDK
                     ENRVNGIALYYRLPMVQVSDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTA
                     RSVRIAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWL
                     KNAVDQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQL
                     RKNQDAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQ
                     AEISSKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIA
                     LANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARANNPEPGFQLIN
                     QRITHSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIA
                     QFTAADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPL
                     TRATDNASVTVKEQNEPLAQVSQWKRYDSRTPVNLETRMALDAFNVALAKDLRVNIRE
                     QASGAYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQEL
                     NEYQQNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVK
                     QYLSHPVNTYTGVLLPK"
BASE COUNT         3028 a         2279 c         2279 g         2729 t
ORIGIN      
        1 catttatgaa gtctgcatta aagaaaagtg tcgtaagtac ctcgatatct ttgatactgg
       61 catctggtat ggctgcattt gctgctcatg cggcagatga tgtaaagctg aaagcaacca
      121 aaacaaacgt cgctttctca gactttacgc cgactgaata cagtaccaaa ggaaagccaa
      181 atattatcgt actgaccatg gatgatcttg gttatggaca acttcctttt gataagggat
      241 cttttgaccc aaaaacaatg gaaaatcgtg aagttgtcga tacctacaaa atagggatag
      301 ataaagccat tgaagctgca caaaaatcaa cgccgacgct cctttcatta atggatgaag
      361 gcgtacgttt tactaacggc tatgtggcgc acggtgtttc cggcccctcc cgcgccgcaa
      421 taatgaccgg tcgagcgccc gcccgctttg gtgtctattc caataccgat gctcaggatg
      481 gtattccgct aacagaaact ttcttgcctg aattattcca gaatcatggt tattacactg
      541 cagcagtagg taaatggcac ttgtcaaaaa tcagtaatgt gccagtaccg gaagataaac
      601 aaacgcgtga ctatcatgac aacttcacca cctattctgc ggaagaatgg caacctcaaa
      661 accgtggctt tgattacttt atgggattcc acgctgcagg aacggcatat tacaactccc
      721 cttcactgtt caaaaatcgt gaacgcgtcc ccgcaaaagg ttatatcagc gatcagttaa
      781 ccgatgaggc aattggcgtt gttgatcgtg ccaaaacact tgaccagcct tttatgcttt
      841 acctggctta taatgctccg cacctgccaa atgataatcc tgcaccggag caatatcaga
      901 agcaatttaa taccggtagt caaacggcag ataactacta cgcttccgtt tattctgttg
      961 atcagggtgt aaaacgcatt ctcgaacaac tgaagaaaaa cggacagtat gacaatacta
     1021 ttattctctt tacctccgat aatggtgcgg ttatcgatgg tcctctgccg ctgaacgggg
     1081 cgcaaaaagg ctataagagt cagacctatc ctggcggtac tcacacccca atgtttatgt
     1141 ggtggaaagg aaaacttcaa cccggtaatt atgacaagct gatttccgca atggatttct
     1201 acccgacagc tcttgatgca gccgatatca gcattccaaa agaccttaag ctggatggcg
     1261 tttccttgct gccctggttg caagataaga aacaaggcga gccacataaa aatctgacct
     1321 ggataacctc ttattctcac tggtttgatg aggaaaatat tccattctgg gataattacc
     1381 acaaatttgt ccgtcatcag tcagacgatt acccgcataa ccccaacacc gaggacttaa
     1441 gccaattctc ttatacggtg agaaataacg attattcgct agtctataca gtagaaaaca
     1501 atcagttagg tctatacaaa ctgacggatc tacagcaaaa agataacctt gccgccgcca
     1561 atccgcaggt cgttaaagag atgcaaggcg tggtaagaga gtttatcgac agcagccaac
     1621 caccgcttag cgaggtaaat caggagaagt ttaacaatat caagaaagca ctaagcgaag
     1681 cgaaataact aaaccttcat gcggcggatt tttccgccgc cttattgagc gagatagcga
     1741 tgcacgttac agccaagccc tccagttttc aatgtaatct caaatgtgat tactgttttt
     1801 accttgaaaa agagtcgcag tttactcatg aaaaatggat ggatgacagc actctgaaag
     1861 agttcatcaa acaatatatc gcagcgtctg gcaatcaggt ctattttacc tggcaaggcg
     1921 gtgaacccac tctggctggc ctggattttt tccgtaaagt tattcactat caacaacgct
     1981 atgcaggcca aaaacgtatt tttaatgcat tacaaacgaa tggcatttta ttgaataatg
     2041 aatggtgtgc ctttctcaaa gaacatgagt ttctggttgg tatctcgatc gatggacccc
     2101 aggagctaca tgaccgttac agacgcagta attctggtaa cggtactttt gcaaaagtga
     2161 tagcagccat cgagcgtctg aaatcatatc aagtagagtt taatacgtta accgtcatta
     2221 ataacgttaa tgtccattac cctcttgagg tttatcattt tttaaaatct atcggcagta
     2281 aacatatgca atttatcgaa ttgctcgaaa ccgggacgcc gaatattgat ttcagtggtc
     2341 atagtgagaa cacattccgt atcattgatt tttctgtgcc tcctacggct tatggcaagt
     2401 ttatgtcgac catttttatg caatgggtta aaaacgatgt gggtgaaatt ttcatccgtc
     2461 agtttgaaag ttttgtcagc cgttttttgg ggaatgggca taccagttgt attttccagg
     2521 agtcctgcaa ggataatctg gttgttgaaa gtaatggaga catttacgaa tgcgaccatt
     2581 ttgtctatcc acagtacaaa attggaaaca ttaataaatc tgaacttaaa acgatgaaca
     2641 gtgtacaact gacagcgcaa aaaaaacgga tttcagcgaa atgtcagcaa tgtgcatata
     2701 aacctatctg caatggcggt tgccctaagc atcgtattac taaagtaaac aatgagactg
     2761 tttcctattt ttgcgaaggt tataaaatcc ttttttcaac catggtacct tatatgaacg
     2821 ccatggtgga gttagccaag aacagagtac cgctttacca cattatggat gttgcaaaac
     2881 aaatggagaa taattaataa cgctccttct ttgctccagt cttagcctcc gtcttatgac
     2941 cttaacaaag cacccgacat aaattcctgt tgggtgtttc gtcataatca tcttcccttg
     3001 cgaaaattct ggcttacgtg gcatgcgtca gggcattagc aatacccttg attatcaata
     3061 gcataaccgt aaccattgaa aataaaaaaa tttctttata tttcaaaatg ttaaatgaca
     3121 cttaaataaa tttcatcagt aaatcattgc catagataat cattggatgg cacaatcaat
     3181 tttcgctaat gataaccatt cccattacgt ttcgtatgtt gatagcaaag tatctctgtc
     3241 tgctgaagcc cttttggctg cgtaagaata ataaaacttc agtgctgtta atcattatta
     3301 tcctcgccat gatccttggc gtggttaaaa ttcaggtctg gttaaacgac tggaataatg
     3361 attttttcaa tgcgctaagc cagaaagaaa ccgataagct ttggcagctt gtcctttggt
     3421 tccctgccct gctggggatc tttgtattaa tatccgtaaa caaaacatgg ttaattaaac
     3481 tgctgactat tcgctggcgc gaatggttaa cagattacta ccttaatcgg tggtttgcag
     3541 ataaaaacta ctacttcacg cagatctacg gcgagcataa aaatacagat aaccctgacc
     3601 aacgtatcgc tgaagacgtt ctcttactga tcagcaaaac gctcagtctt tcatttggct
     3661 tcatccagtc acttagcatg ctgatcacct ttaccgttat tctttggcaa agcgcgggta
     3721 cgctctcatt cactgttggt ggaacagaat ggaatatcca ggggtatatg gtctataccg
     3781 ttgtgctcat tgtgatcggt ggaactttat ttactcataa ggttggtaaa cgtattcgtc
     3841 cgcttaatgt ggaaaagcaa cgtagcgaag cgacattttg aactaatctt gtgcagcata
     3901 ataagcaggc tgaactgatt gcgctaagca atgctgagtc tctacaacgc caggagttga
     3961 gcgacaattt tcataccatt aaagagaact ggcatcgttt aatgaatcgt cagcgctggc
     4021 ttgattactg gcagaatatt tattcgcgct cgcttagcgt tcttccctac tttctgttat
     4081 tgccacagtt tattagcgga cagatcaatc tgggcggact gatgaaatcg cgccaggcat
     4141 ttatgctggt atcgaacaat ttaagctggt ttatttataa atatgacgaa cttgctgaac
     4201 tggctgcggt tatcgatcgt ttgtatgagt tccatcaact caccgaacag cgccctacga
     4261 ataagcctaa aaattgccaa catgcggtac aagtggctga cgcgagtatt cgtactcctg
     4321 ataataagat catattagag aacctgaact ttcatgtttc gccaggcaaa tggctattac
     4381 tgaaaggtta ctctggtgcg ggaaaaacca cactgcttaa aacattatcc cactgctggc
     4441 cgtggttcaa aggtgatatt tcttctcctg ctgacagttg gtatgtgtca caaacaccgt
     4501 taatcaaaac cggcttactg aaagagatta tttgtaaagc acttcccctg cccgtagacg
     4561 ataaatcgtt gagcgaaata ctgcatcagg ttggtctggg gaaattggct gcgcgtattc
     4621 atgactacga tcgctgggga gatattcttt ccagcggcga aaaacaacgt atcgccctgg
     4681 cacgattaat tttacgacgt ccgaaatgga tatttcttga cgaaactacc tctcatcttg
     4741 aggaacaaga ggctatccgc ttactgcgtt tagtgcgtga aaaactaccc acaagcggcg
     4801 tcattatggt tacacatcaa cccggtgtct ggaacctggc tgatgatatt tgtgacatta
     4861 gcgcggtttt ataaaaacaa taaaagccct gctgtacgga gataaaaata aatgaagcga
     4921 gttcttattc ctggcgtcat tttatgtggc gctgatgtgg cgcaggccgt cgatgacaaa
     4981 aacatgtaca tgcatgtttt tgaagagatg acggtctatg ctcctgtccc tgtacccgta
     5041 aacggcaaca cgcattacac cagtgaaagc atcgagcgtt taccgaccgg gaatggcaat
     5101 atcagcgatc tgctgagaac caaccctgcg gtacgcatgg attcaacgca aagtacctca
     5161 ttgaaccagg gagatattcg ccctgagaaa atctctattc acggtgcgtc gccctaccag
     5221 aatgcctatt tgattgacgg tattagtgca actaataacc tgaacccagc gaatgagtcc
     5281 gatgccagta gtgcaaccaa tattagcggg atgtcacagg ggtattatct tgatgtcagc
     5341 ttactggaca atgtgacgct ttatgacagt tttgtgccgg ttgaatttgg tcgcttcaat
     5401 ggcggggtaa ttgatgcaaa gatcaaacgc ttcaacgctg atgatagcag cgtgaaactg
     5461 ggttaccgca ctacgcgttc ggactggtta acatcgcata tcgatgagaa taacaagagc
     5521 gcatttaatc aaggctcttc aggaagtact tattactccc cagattttaa aaagaacttt
     5581 tatactttgt catttaatca ggaactcgct gataacttcg gcgttaccgc cggtttatcg
     5641 cgccgccagt ctgatatcac ccgcgcggat tatgtttcga atgacggcat tgtcgccggt
     5701 cgggcacagt ataaaaacgt tatcgatacc gcagtgagca aatttacctg gtttgccagt
     5761 gaccgcttta cccacgattt aaccttaaaa tataccggct ccagccgtga ttataatacc
     5821 agcaccttcc cggagtctga tcgcgaaatg ggtaataaat cctatggtct ggcatgggat
     5881 atggatacgc agctcgcatg ggccaaactg cgtactaccg ttggttggga tcatattagt
     5941 gattataccc gtcacgatca tgacatatgg tacaccgaac tttcatgtac atatggtgat
     6001 atttcagggc gttgcacccg tggcggatta ggacacattt cccaggctgt agataattac
     6061 accttcaaaa cacgcctgga ctggcaaaaa tacgccgtgg gtaatgtttc gcatcaaccc
     6121 tacttcggcg cggaatacat ctattccgat gcatggactg aacgccataa ccagtctgaa
     6181 tcctatgtga ttaatgctgc aggaaagaaa actaaccata ccatttacca taaaggtaaa
     6241 ggcagtctgg gaattgacaa ctacacgctg tatatggcgg atcgcattag ctggcggaat
     6301 gtgtcgttaa tgcccggtgt gcgatatgac tatgacaact atctgtcaaa ccacaatatc
     6361 tccccgcgat ttatgacgga atgggatatt tttgctgatc aaacctcaat gattactgcc
     6421 ggttataacc gttactatgg cgggaatatt cttgatatgg gattacgtga tatccgcaat
     6481 agctggacgg aatcggtatc aggcaataaa accctgacgc gttatcagga tttgaaaaca
     6541 ccttataacg atgaactggc aatggggttg cagcagaaaa tcggaaagaa cgttattgcg
     6601 cgtgcaaact atgtttatcg tgaagcgcat gatcaaatca gtaaaagcag tcgtaccgac
     6661 agcgcgacta aaaccaccat tactgaatat aacaacgacg gcaaaaccaa aacgcattcg
     6721 ttcaacctca gttttgaact ggccgaaccc ctgcatatca gccaggtaga tattaacccg
     6781 caaattgtct ttagctatat caagagcaag ggcaacttgt cgttaaacaa tggttatgag
     6841 gagagcaata ccggtgataa ccgggtggta tataacggta atctggtctc ttacgatagc
     6901 gttccagtgg cggattttaa taatccacta aagatctcct taaacatgga tttcacgcat
     6961 caaccgagcg ggttggtgtg ggcgaatacg ttgacctggc aagaagcgcg taaagctcgc
     7021 attatcctgg gtaagacgaa tgcgcaatac atcagcgaat attcagatta caagcagtat
     7081 gttgacgaga gactggatag cagcctgacc tgggacaccc gcttgtcctg gacgccacaa
     7141 tttctgaaac aacaaaacct gacgatcagt gccgatattc tcaatgtact ggatagcaaa
     7201 accgctgttg atacaacgaa taccggcgtg gcgacctacg ccagtggccg tactttctgg
     7261 cttgatgtca gcatgaaatt ttaatagttc ggattttact ttaccgggta aattcgcccg
     7321 gttttcgcat ggagataatt atgagaaacc tctgtttctt actgacgtta gtggcaactc
     7381 tgttgctccc cgggcgactg attgccgccg ccttaccgca ggatgaaaag ttaattaccg
     7441 ggcaactgga caatggcttg cgatatatga tttatccgca tgctcaacca aaggatcagg
     7501 taaatttatg gctgcaaatt cataccggtt cattgcagga agaagacaat gagcgcggcg
     7561 tggctcattt tgtagaacat atgatgttta acggcacaaa aacatggccg ggtaataaag
     7621 tcatcgaaac atttgagtca atgggcctgc gctttggtcg cgatgttaat gcctatacca
     7681 gctatgacga aacggtgtat caggtaagtt tgccgactac gcagaaacaa aatctgcaac
     7741 aagtgatggc aatcttcagt gaatggagta atgccgcaac ctttgaaaaa ctcgaagtag
     7801 acgctgaacg cggcgtaatt actgaggaat ggcgtgccca tcaggatgcg aaatggcgca
     7861 cctctcaggc gcgccgccct ttcctgcagg ctaatacccg taatttagac cgtgaaccta
     7921 tcggcctgat ggatactgtc gctacggtca caccggcaca attgcgccaa ttttatcaac
     7981 gctggtatca accaaataat atgaccttta tcgtggtcgg cgatattgac agtaaagaag
     8041 cgctggcgct gattaaggat aatttaagta agcttccggc taacaaagca gctgaaaatc
     8101 gcgtctggcc gacaaaagcc gaaaaccacc tgcgctttaa tatcattaat gataaagaaa
     8161 accgggtgaa cggcatcgca ctctattatc gcctgccaat ggtgcaagtg agcgatgagc
     8221 aaagctttat cgaacaagct gaatggagca tgttggttca gctgttcaat cagcgtctgc
     8281 aggaacgcat acagtcgggc gagttgaaga ctatttctgg cggcactgcg cgcagcgtta
     8341 gaattgcacc cgattatcag tcgctgtttt tccgtgtaaa tgcacgagac gataatatgc
     8401 aggatgctgc gaatgcatta atggcagagt tggcaactat tgatcagcat ggtttttctg
     8461 ctgaagaact cgatgatgtc aaatctaccc gtctcacctg gctgaaaaat gcggttgatc
     8521 agcaagctga acgtgattta cgtatgctga ccagtcgcct ggcatccagc tcattaaata
     8581 atacgccgtt cttgtcgccg gaagagacat atcaactttc gaaacgtctg tggcagcaaa
     8641 ttaccgtgca aagtctggcg gaaaaatggc agcagttaag aaagaaccag gatgcatttt
     8701 gggagcaaat ggtaaacaat gaggttgctg ccaaaaaagc attgtctcct gcggctatcc
     8761 tggcgctgga aaaagagtac gccaacaaaa agctggcggc ttacatcttc ccaggcagaa
     8821 atttatcgtt aacagtagac gctgacccac aggcggaaat tagcagcaaa gaaacgctgg
     8881 ctgagaatct gacatcatta acactttcca atggtgccag ggttattctg gcaaaatctg
     8941 cgggtgaaga gcaaaagcta caaattactg ccgtatctaa taaaggcgat ttaagtttcc
     9001 ctgcgcagca aaaatcactt atcgcgctgg caaataaagc agtaagcgga agcggcgttg
     9061 gtgaactctc ctcttccagc ctgaaacgct ggagtgcgga aaactcggta accatgagca
     9121 gtaaagtcag tggcatgaat acattgctct ctgttagcgc gcgggctaat aaccctgaac
     9181 ctggtttcca gttgattaac cagcgaatca cccacagcac gattaacgat aatatttggg
     9241 catcgctaca aaatgctcaa attcaggcgt tgaaaacgct cgaccagcgt ccagcggaga
     9301 aattcgccca gcagatgtat gagacgcgct atgctgatga ccgcacgaaa ttactgcaag
     9361 aaaatcagat tgcacagttt actgccgcag atgcgctggc tgccgatcgc cagttgtttt
     9421 catctccagc ggatatcacg tttgtcattg tcggtaatgt cgcagaagac aaactcgtgg
     9481 cgttaattac gcgttactta ggatcaatca aacactctga ttcgccatta gccgcaggta
     9541 aaccattaac tcgcgcgacg gacaacgcat cggttactgt aaaagaacaa aatgaacctt
     9601 tggcacaggt ttcacagtgg aagcgttatg attcccggac acctgtaaat ctggagacgc
     9661 gtatggcgct cgatgctttt aacgtcgcac tggcaaaaga tctacgcgtt aatattcgtg
     9721 aacaggcctc tggggcatac agcgtttctt ctcgcctctc ggttgatcct caggccaaag
     9781 atatcagtca tttgctggct tttacttgtc aaccggaacg acatgatgaa ctgttaacgt
     9841 tagcgaatga agtgatggtt aagcgtctgg ctaaagggat cagtgagcaa gaactgaacg
     9901 aataccagca aaacgttcag cgcagcctcg atatccaaca gcgtagcgtt caacaattag
     9961 cgaacaccat tgtaaatagt cttattcaat atgacgatcc tgcagcatgg actgagcagg
    10021 agcaattgtt gaaacaaatg acggtagaga atgttaacac tgccgttaaa caatatcttt
    10081 ctcatccggt gaatacctat accggagtat tgttgccaaa ataataacag ccccgtcaac
    10141 acctcgttgg cggggatttt agcaatattc gctattttta tgtaataatt ttataaatgc
    10201 gttcaaaata ataaccaagt actaatagtg atattttaag gtctgatttt tacgtgataa
    10261 ttcaggaggc gtagaaagcg cataaaaata acaacataaa acactttacc accac
//
LOCUS       DACBVI010000156        10280 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.157, whole genome shotgun sequence.
ACCESSION   DACBVI010000156 DACBVI010000000
VERSION     DACBVI010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.157"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            72..647
                     /gene="ssuE"
                     /locus_tag="IU325_18990"
     CDS             72..647
                     /gene="ssuE"
                     /locus_tag="IU325_18990"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HAP5811091.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKNFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            640..1599
                     /locus_tag="IU325_18995"
     CDS             640..1599
                     /locus_tag="IU325_18995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP5811092.1"
                     /translation="MRNIIQLALAGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQTKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVPENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1596..2741
                     /gene="ssuD"
                     /locus_tag="IU325_19000"
     CDS             1596..2741
                     /gene="ssuD"
                     /locus_tag="IU325_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HAP5811093.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2753..3544
                     /gene="ssuC"
                     /locus_tag="IU325_19005"
     CDS             2753..3544
                     /gene="ssuC"
                     /locus_tag="IU325_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HAP5811094.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3541..4308
                     /gene="ssuB"
                     /locus_tag="IU325_19010"
     CDS             3541..4308
                     /gene="ssuB"
                     /locus_tag="IU325_19010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HAP5811095.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLQGQWRDAARRALAAVGLENRAREWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGESETRLRKQG"
     gene            complement(4350..6962)
                     /gene="pepN"
                     /locus_tag="IU325_19015"
     CDS             complement(4350..6962)
                     /gene="pepN"
                     /locus_tag="IU325_19015"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HAP5811096.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     NGASDAPLRLDGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIVNEISPAT
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7228..8430
                     /gene="pncB"
                     /locus_tag="IU325_19020"
     CDS             7228..8430
                     /gene="pncB"
                     /locus_tag="IU325_19020"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP5811097.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLADFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8599..9999
                     /gene="asnS"
                     /locus_tag="IU325_19025"
     CDS             8599..9999
                     /gene="asnS"
                     /locus_tag="IU325_19025"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP5811098.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2310 a         2676 c         2770 g         2524 t
ORIGIN      
        1 tctctccgac agactgaccg aattgttatc aatgttaaca aaacaagaac aattggttat
       61 aaggagagag tatgcgtgtc atcaccctgg cgggtagtcc tcgttttcct tctcgctcca
      121 gctccttgct ggaatatgcg cgggaaaaac tgaatggcct ggatgtagag gtttatcact
      181 ggaatctgca aaactttgcc ccggaagatc tgctttatgc tcgtttcgat agtccggcac
      241 tcaagaactt caccgaacaa ctgcaacagg ccgatggact gatagtcgcc acgcctgtgt
      301 ataaagccgc ctattccggt gcgctgaaaa ccctgctcga cctgctgcca gaacgcgctt
      361 tgcaaggcaa agtggtactg ccactggcga cgggcggcac ggtggctcat ctgctggcgg
      421 tcgattacgc ccttaaacca gttttaagtg cgctgaaagc ccaggagatc ctgcacggcg
      481 tgtttgccga tgactcgcaa gtaattgatt accaccacaa accccagttc acgccaaatc
      541 tgcaaacccg tcttgatacc gcgctggaaa ctttctggca ggcattgcac cgtcgggatg
      601 ttcaggttcc tgaccttctg tctctgcgag gtaatgccca tgcgtaacat cattcaactg
      661 gcgctggcgg gattacttag cgtctctacg attgcggttg ctgcagaatc ctctcctgaa
      721 gcgttacgta taggctatca gaaaggcagt attggtatgg tgctggcaaa aagccaccag
      781 ttactggaaa aacgctatcc gcaaaccaaa atctcctggg tggagttccc cgctggccca
      841 cagatgctgg aggcgttaaa cgttggcagt attgatctcg gcagtaccgg agatattccg
      901 ccaatctttg cccaggctgc cggggctgat ttggtgtacg tgggcgtcga gccgccgaag
      961 cccaaagccg aagtgattct ggtgccagaa aacagcccga tcaaaaccgt agccgatctt
     1021 aaaggtcaca aagttgcctt tcagaaaggt tccagctcgc acaatctttt actacgcgca
     1081 ctacgccagg ccgggcttaa attcacggat atccagccca cttacctgac gcctgccgat
     1141 gcccgcgccg cgttccagca aggtaacgtc gacgcatggg ctatctggga tccctactac
     1201 tccgctgcat tattacaggg cggcgtgcgg gtgctaaaag acggtaccga tctcaatcaa
     1261 accggatcgt tttatctggc tgcccgtccg tatgcagaaa aaaacggcgc ttttattcag
     1321 ggcgtactgg caacctttag tgaggccgat gcgttaaccc gcagccagcg cgagcaaagc
     1381 atcgctttac tggcaaaaac gatgggctta ccggccccgg tgattgcctc ttatttagat
     1441 catcgccccc ctaccaccat caaaccggtt aacgccgaag ttgccgcctt acagcagcaa
     1501 acggcagatc tgttttatga aaatcgtctg gtgcctaaaa aagtcgatat tcgccagcgc
     1561 atctggcagc ccactcaact ggaaggaaaa caattatgag tctgaatatg ttctggtttt
     1621 taccgaccca tggtgacggg cactatctgg gaacggaaga aggttcacgt ccggttgatc
     1681 acggttatct gcaacaaatt gcgcaagcgg cggatcgtct gggttatacc ggtgtgctga
     1741 tcccaacagg acgatcgtgt gaagatgcgt ggctggtggc ggcgtcgatg atcccggtga
     1801 cgcaacggct gaagtttctt gtcgccctgc gtcccagcgt aacctcacct accgttgccg
     1861 cccgccaggc cgccacgctt gaccgtctct cgaatggtcg tgcgttgttt aatctggtca
     1921 caggcagcga tccacaagag ctggcaggcg acggcgtgtt ccttgatcat agcgagcgct
     1981 acgaagcctc ggcggaattt acccaggtct ggcggcgctt attgctgggg gaaaccgtca
     2041 atttcaacgg taaacatatt catgtgcgcg gagccaaact actcttcccg ccgattcaac
     2101 agccgtatcc tccgctttac tttggcggat cgtcagatgt cgcccaggag ctggcggcag
     2161 aacaagttga tctctacctc acctggggcg aaccgccgga acttgttaaa gagaaaatcg
     2221 aacaagtgcg ggcgaaagct gccgcgcatg gacgcaaaat tcgtttcggt attcgtctgc
     2281 atgtgattgt tcgtgaaacc aacgacgaag cgtggcaggc cgccgagcgg ttaatctcgc
     2341 atcttgatga tgagaccatc gccaaagcac aagccgcatt tgcccggacg gattccgtag
     2401 gacaacagcg aatggcggcg ctacataacg gcaagcgcga caatctggag atcagcccca
     2461 atttatgggc gggcgttggc ttagtgcgcg gcggtgccgg gacggcgctg gtgggcgatg
     2521 gtccgacggt cgccgcgcga atcaacgaat acgctgcact tggcatcgac agttttgtgc
     2581 tttcgggcta cccgcatctg gaagaagcgt atcgggttgg cgaattgctg ttcccgcatc
     2641 tggatgtcgc catcccggaa attccccagc cgcagccgct gaatccgcaa ggcgaagcgg
     2701 tggcaaatga ttttatcccc cgtaaagtcg cgcaaagcta aggaacacga taatggcaac
     2761 gccagtgaaa aagtggttat tgcgcgttgc cccctggttt ttaccggtgg gcatcgtggc
     2821 ggtgtggcaa ctggcctcct cagttggctg gctgtcgacg cgtattttgc cctctccgga
     2881 aggagtggtg acggcgttct ggacgctctc cgccagcggc gaattgtggc agcatctggc
     2941 gatcagctcc tggcgggcgc tcattggctt ttcaattggc ggatcgctgg gcttgattct
     3001 gggactgatt agcgggctgt cgcgctgggg agaacggctg ctggatacct caattcagat
     3061 gttgcgcaac gtgccgcatc tggcgctgat cccgctggtg attttgtggt ttggcattga
     3121 tgaatccgca aaaatctttc tggtggcgct tggcacgcta ttccccattt atatcaacac
     3181 ctggcatggc atccgtaata tcgatcgcgg gctggtggag atggcgcgta gctatggatt
     3241 atccggcata ccgctgttta tccatgtgat cctgcctggc gccctgccct caattatggt
     3301 cggcgtgcgt tttgcgttgg gcctgatgtg gctgacactg atcgttgccg aaactatttc
     3361 tgccaattcc ggcattggtt atctggcgat gaatgcgcgg gagtttctgc aaacggacgt
     3421 ggtggttgtc gctattattc tttacgccct gctcggcaaa cttgctgacg tcagcgcgca
     3481 gctgttagag cgcctctggt tgcgctggaa cccggcttat catttgaagg aggccacggt
     3541 atgaatactg ctcgtctgaa ccagggcaca ccattgttgc tcaatgcagt aagcaaacat
     3601 tacgcagaaa atatcgtcct gaaccaactg gatttacata ttccggcagg tcagtttgtg
     3661 gcggtggtgg gccgcagcgg tggtggcaaa agtaccttgc tgcgcctgct ggcaggtctg
     3721 gaaacgccaa ccgcaggcga tgtgttagcg ggtaccacac cgctggctga gattcaggaa
     3781 gacacgcgaa tgatgtttca ggatgcgcgt ctgctaccgt ggaaatcggt gattgataac
     3841 gttgggctgg gccttcaagg tcagtggcgc gatgccgcgc gtcgggcgct ggctgcggta
     3901 gggctggaga atcgcgcgcg ggaatggcct gccgcacttt ctggcgggca gaaacagcgt
     3961 gtggcgctgg caagagcgtt gattcatcga ccgggattgt tgttgcttga tgaaccgctc
     4021 ggggcgctgg acgccttaac gcgactggag atgcaggatt tgattgtgtc actttggcag
     4081 gagcacggct ttaccgtact gttggtgacg catgatgtga gcgaagcggt agcaatggct
     4141 gaccgggtgt tgttaattga agagggaaaa attggtctgg atttgacggt ggatatcccc
     4201 cgtccgcgcc ggttagggtc ggtgcgatta gcggagctgg aagcggaagt gttgcaacgg
     4261 gtgatgcagc gtggtgaatc tgaaactcgc ctgagaaaac aggggtaaat tcccgaatgg
     4321 cggcgctatt gccgccattc ggttattttt taagccagtg ctttagttat cttctcgtac
     4381 agatcgccag agagattttc cagccctttc aactgttcca gcgccgcgcg cattttctcc
     4441 tgacgtttgg catcataacg tttcaggcga atcagcggct caatcaggcg tgaagccacc
     4501 tgcgggttac ggctgttgag atcggttagc atttccacca ggaactggta accgctgcca
     4561 tcttcggcat ggaacgctgc cgggttgctg cccgcaaacg caccaatcaa cgaacgaata
     4621 cggttcgggt tgctcatggt aaatgagcga tgctgcaaca ggccacgcac cgtctccagc
     4681 acattcgccg ccgggctggt ggcttgcagg ataaaccatt tatccatcac cagaccatcc
     4741 tgatgccact tgtcgtcgta ctcctgcatc agcgcgtcac ggcaaggcag ctgtgcagca
     4801 accgccgcag aaagcgctgc cagcgcatcg gtcatattgt ttgcttcatg gtattgcttg
     4861 ctcaccagca cgtcagccag atgcgtttca ccaaaagcaa ggaagcgcag gcaggcatta
     4921 cgcagagtgc gttttgcaat atcttcatgc tcaacacggt actcgctctg gtaattcgcg
     4981 ttgtaaatag ccagtagctc atccgccagt tcagtcgcca gagtacgagt gagtgcttcg
     5041 cgtacttcag caatagcaat cgggtcgatg atatcgaaca gttctgccat ttcattgacg
     5101 gaaggcagcg tcaggatttc tgccgccagt gccggatcaa tcttctcatc aagcagcacc
     5161 gcgcggaaag cgtcagccac atgcaccggc agagacagcg gctgcccttg ctgatgacgc
     5221 gcgacgttca gcttgatgta ggttgccagc aggctttgcg ccgcatccca gcgggagaaa
     5281 tcattacgcg catgacgcat caggaaggtc agttgctgat cgctccactt atattccagt
     5341 ttcactggcg cagagaattc gcacagcagc gcaggcaccg gctggaagta gacattatca
     5401 aagacaaagg tctgttccgc ctgggtgacg ttcagcaccg aattgaccgg atgaccgcct
     5461 ttctgcaacg ggatcacttt gccttcgtta tcatacagtt cgatggcaaa cggaatatgc
     5521 agcggctgtt tttctgcctg atccggcgta gctggcgtgc gctggctgat ggtcagggtg
     5581 tactgctcgg tttctggatt gtagtcgtct ttgacggtca caaccggcgt accggactgg
     5641 ctgtaccaac ggcggaaatg agagagatcg acattcgatg catcttccat cgcctgcaca
     5701 aagtcgtcac aggtcgctgc actaccatca tgacgctcga aataaagctg catccctttc
     5761 tggaagtttt cttcgcccag cagggtgtgg atcatgcgaa tcacttccgc gcccttctcg
     5821 taaacggtca gggtgtagaa gttgttcatc tcaatgacca tatccgggcg gatcgggtgc
     5881 gccatcgggc tggcgtcttc cgcaaactgc aatccgcgca tggtgcgcac gttgttgata
     5941 cggtttaccg cgcgggaacc aaggtcagag ctgaactcct gatcgcggaa cacggttaaa
     6001 ccttctttca ggctgagctg gaaccagtcg cggcaggtca cacggttacc ggtccagttg
     6061 tggaaatatt cgtgaccaat aacacgctca atatcgaggt aatctttatc ggtggcggtg
     6121 tcggtacggg ccagcacata tttggagtta aagatattca gacctttatt ctccattgcg
     6181 cccatattga agaaatccac cgcgacgatc atatagatgt cgaggtcata ctccaggccg
     6241 aagcgttctt catcccattt catggagttt ttcagcgagg tcatcgccca cggcgcacga
     6301 tcaaggttgc cgcgatcgac gtacagctcc agcgcaactt cgcgaccaga acgcgtggtg
     6361 aaggtgtcgc gcagcacgtc gaagtcgcct gccaccaacg caaacaggta gcatggtttc
     6421 gggaacgggt cctgccactg tacccaatga cgtccgtttt ccagttcgcc ttgcgcaacg
     6481 cggttaccgt tggaaagcag gaagggatat ttggttttat cggcaataat tttggtggta
     6541 aaacgcgcca gtacgtccgg gcggtcaaga taatacgtaa tatggcggaa accttcggct
     6601 tcacactgag tgcaaagcgc atcgcctgac tgataaagcc cttccagcgc ggtattcgtc
     6661 gccgggctaa tttcattaac gatcttaagc gtaaaacgct ccggcaaatt actgatgacc
     6721 agtgcgccct cttcttcttt ccaggcggtc cacggctcat cattaatatg aacagaaacc
     6781 agtttaagat cttcgccatc aagacgaagc ggagcatctg atgcaccatt acggacagcc
     6841 tggctgaccg cggtaacgac cgtcttttgc gcgtccaggt caaaggtcaa gtcaatatca
     6901 gtaatctggt aatccggcgc acgataatcg tgacggtatt tggcttgtgg ctgttgagtc
     6961 ataaataacc tttagcatct tttatagagt ctggtgttca gtctattcct gttgcgtaaa
     7021 tcgcgctatg cagaatcttc atcttttcag gtacaaacgc ctttattgct acatttttat
     7081 aacatgcacc gcgtaatgcc atcgaccaga aaggtggcat atggtgtgat cggggttcaa
     7141 taaattgcga aacaaggtat actccagcag ttcctgaaga tgtttattgt actaaacgct
     7201 cctgtacgag gacgctactg cgcacctatg acacaattcg cttctcctgt tctgcactcg
     7261 ttgctggata cagatgctta taagttgcat atgcagcaag ccgtgtttca tcactattac
     7321 gatgtgcatg tcgcggcgga gtttcgttgc cgaggtgacg atctgctggg tatttatgcc
     7381 gatgctattc gtgaacaggt tcaggcgatg cagcacctgc gcctgcagga tgatgaatat
     7441 caatggctct cagccctgcc tttctttaag gccgactatc ttaactggct acgcgagttc
     7501 cgctttaacc cggaacaagt caccgtgtcc aacgataatg gcaagctgga tattcgttta
     7561 agcggcccgt ggcgtgaagt catcctctgg gaagttcctt tgctggcggt tatcagtgaa
     7621 atggtacatc gctatcgctc accgcaggcc gacgttgcgc aagccctcga cacgctggaa
     7681 agcaaattag ccgacttctc ggcattaacc gccggtcttg atatgtcgcg cttccatctg
     7741 atggattttg gcacccgccg acgtttttct cgtgaagtac aagaaaccat cgtgaaacgt
     7801 ctccaacagg aatcctggtt cgtgggcacc agcaactacg atctggcgcg tcggctttcc
     7861 ctcacgccaa tgggaacaca agcacacgaa tggttccagg cgcatcagca aatcagcccg
     7921 gatctagcca acagccagcg agctgcactt gctgcctggc tggaagaata tcccgaccaa
     7981 cttggcattg cattaaccga ctgcatcact atggatgctt tcctgcgtga tttcggtgtc
     8041 gagttcgcca gtcgctatca gggcctgcgt catgactccg gcgacccggt tgaatggggt
     8101 gaaaaagcca ttgcacatta tgagaagctg ggaattgatc cacagagtaa aacgctggtt
     8161 ttctctgaca atctggattt acgcaaagcg gttgagctat accgccactt ctcttcccgc
     8221 gtgcaattaa gttttggtat tgggactcgc ctgacctgcg atatccccca ggtaaaaccc
     8281 ctgaatattg tgattaagtt ggtagagtgt aacggtaaac cggtggcgaa actttctgac
     8341 agccctggca aaactatctg ccacgataaa gcgtttgttc gggcgctgcg caaagcgttc
     8401 gaccttccgc atattaaaaa agccagttaa tatcatcagg gagctaatcg gctccctttt
     8461 tttaccttta attccgaaat ctttcgctgc atttgcgaat tctgcttgtc tgattgcaga
     8521 tgccaggtaa cataggtatc cccccattta cgggtggaca agtgtttatt ttttccgact
     8581 attaacagag agaatattat gagcgttgtg cctgtagccg acgtactcca gggccgcgta
     8641 gccgttgaca gcgaagtcac cgtgcgcgga tgggtacgta cccgccgaga ttcaaaagct
     8701 ggcatctcct tcctcgccgt ttatgacggt tcctgctttg atcctgtaca ggctgtcatc
     8761 aataattctc tgcccaatta caatgaagac gtcctgcgtc tgaccaccgg ttgctcggtc
     8821 attgtgacgg gtaaagtcgt ggcgtcgccg ggccaggggc aacaatttga aattcaggcc
     8881 agcaaggttg aagttgctgg ttgggttgaa gatccagaca cttacccgat ggcggcaaaa
     8941 cgccacagca ttgagtatct gcgtgaagtc gctcacctgc gtccgcgcac aaacctgatt
     9001 ggtgccgtcg cgcgcgttcg ccatacgctg gcgcaggcgc tgcatcgctt ctttaacgag
     9061 cagggattct tctgggtttc aacgccactg attactgcgt ctgataccga aggggctggc
     9121 gaaatgttcc gcgtttctac gctggatctg gaaaacctgc cgcgtaacga tcagggcaaa
     9181 gtggatttcg acaaagactt ctttggtaaa gagtctttcc tgaccgtatc tggccagttg
     9241 aacggcgaaa cctacgcttg cgcattatcc aaaatttata ccttcggccc gactttccgt
     9301 gctgaaaact ccaacaccag ccgtcacctg gcggaattct ggatgctgga gccggaagtg
     9361 gcgtttgcta acctgaacga tatcgcgggt ctggctgaag ccatgctgaa atatgtcttc
     9421 aaagcggttc tcgaagaacg cgctgacgac atgaaattct tcgctgaacg cgtagataaa
     9481 gatgccgttt cacgtctgga gcgcttcatt gaagccgatt ttgcgcaggt ggattacacc
     9541 gacgcagtga ccattctcga aaactgcggc aggaagtttg aaaacccggt ttactggggc
     9601 gtcgatctct cttctgagca tgagcgttat ctggcggaag aacactttaa agcaccggta
     9661 gtggttaaaa actatccgaa agatattaaa gcgttctata tgcgcctcaa cgaagacggt
     9721 aaaaccgttg cggctatgga cgttctggct ccgggcatcg gtgagatcat tggtggctcc
     9781 cagcgtgaag aacgtctgga cgtgctggac gagcgtatgc tggaaatggg cctgaataaa
     9841 gaagattact ggtggtatcg cgatctgcgt cgctacggta ctgttccgca ttcaggtttc
     9901 ggtcttggtt ttgaacgcct gattgcttac gtaactggcg tgcaaaacgt gcgtgatgtt
     9961 attccgttcc cacgtactcc gcgtaacgcc agcttctaat ttagcgtctt caagagccag
    10021 cgcccgctgg ctcttttttt atcctttctg tcaagttatc tgtttgttaa gtcaagcaat
    10081 ctatttgcaa ccctgccata acgatcatcc tgttacggaa tattacactg caacatttgc
    10141 gcacaaaaaa taatccgcat tcttattgcg gattagtttt ttcttagctg atagcacaat
    10201 tttcatacta tttttggcgt tctggatgtc tgaacgagga tttagtgcca ggtcgataaa
    10261 gtttccatca gaaacaaaat
//
LOCUS       DACBVI010000157        10266 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.158, whole genome shotgun sequence.
ACCESSION   DACBVI010000157 DACBVI010000000
VERSION     DACBVI010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.158"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            183..1091
                     /locus_tag="IU325_19030"
     CDS             183..1091
                     /locus_tag="IU325_19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAP5811099.1"
                     /translation="MKAPVSITSFRPQKSIAIVIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIESLKERLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1136..3127)
                     /gene="tkt"
                     /locus_tag="IU325_19035"
     CDS             complement(1136..3127)
                     /gene="tkt"
                     /locus_tag="IU325_19035"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP5811100.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIDAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAEQLFEEFGFTVDNVVAKAKALL"
     gene            3405..4163
                     /locus_tag="IU325_19040"
     CDS             3405..4163
                     /locus_tag="IU325_19040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HAP5811101.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(4211..5131)
                     /gene="speB"
                     /locus_tag="IU325_19045"
     CDS             complement(4211..5131)
                     /gene="speB"
                     /locus_tag="IU325_19045"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HAP5811102.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5267..5998)
                     /locus_tag="IU325_19050"
     CDS             complement(5267..5998)
                     /locus_tag="IU325_19050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811103.1"
                     /translation="MKKWKVRSALIALAVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERVWRDPAEMIVISYSTTVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNKGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NAMPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(6144..8120)
                     /gene="speA"
                     /locus_tag="IU325_19055"
     CDS             complement(6144..8120)
                     /gene="speA"
                     /locus_tag="IU325_19055"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HAP5811104.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(8129..8260)
                     /gene="yqgB"
                     /locus_tag="IU325_19060"
     CDS             complement(8129..8260)
                     /gene="yqgB"
                     /locus_tag="IU325_19060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HAP5811105.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            8396..8611
                     /locus_tag="IU325_19065"
     CDS             8396..8611
                     /locus_tag="IU325_19065"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811106.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            8915..10069
                     /gene="metK"
                     /locus_tag="IU325_19070"
     CDS             8915..10069
                     /gene="metK"
                     /locus_tag="IU325_19070"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP5811107.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         2551 a         2734 c         2588 g         2393 t
ORIGIN      
        1 attttgattg ttgttgtatt tgtgatgcat atcgccattt atttattgaa tgtccgaaaa
       61 catacttttt taatgcaatg ttttttcgct ataccccttt atagattctg aattctcacc
      121 tgtcggctat tttctcctca cctcatgtga catattcccc actttactca aggaagattc
      181 atatgaaagc acctgtttca ataacctcat ttcgcccaca aaaatctatt gctatcgtca
      241 ttggcgtgct tgccgtggtt gtgctgccat ttctctcgta ttacaccgtt aatgaaggtg
      301 aacgcggcat tttgctgcgc tacggaaaaa tcgtaaaagt tgccgagccg ggtctgggtt
      361 ttaagatccc ttttatggaa agcgtggaga aaatttccac gcgtaatcag gctgtggttt
      421 atcagggatt acaggcctac agccgcgacc agcaaccggc acaaatgaca gtatcagtca
      481 gcttccacat taaaccttca gaagctggcg cagtttatac aacctacaac accattgaat
      541 ctttaaaaga gcgtctgatc gtccgccagc tgccaaccca actggagaac gttttcgggc
      601 aatatacagc catcagcgcg gtacaggatc gtacaaaact ggttcaggat ctgcaaaatg
      661 caatgcgtaa agccgttgtt ggcccggtgg tgattgatgg tgttcagatt gaaaatattg
      721 atttttctga cgcttatgaa aaatcgattg aagatcgaat gaaagcagaa gtcgccattg
      781 ccacacgtaa gcaaaatctg gaaacagaga aaatccaggc gcaaatagcg gtaactcagg
      841 cccaggcaga agcagacagt aaactggcgg cagccaaagc agaagctgag actattcgcg
      901 taagaggcgc ggcagaggct gaaactattc gtttaaaaag tgcggctgaa gcagaagcga
      961 tccgcctgcg cggtgaagct ctgcgggata atcccggact tgttgccctc accactgctg
     1021 agcgttggga cggtaagcta cccgatacca tgatccccgg cagtacagtt ccgtttatct
     1081 ccaccaagta aaaaagggcc gctttcgcgg cccttctcac aaagcatctt accgattaca
     1141 gcagcgcttt cgctttcgca acaacgttgt caacggtgaa accgaactct tcaaacagtt
     1201 gctctgcagg agcagattca ccgaaggtgg tcatgccgac gatagcaccg ttcaggccaa
     1261 catacttgta ccagtagtca gcaatgccag cttccaccgc aacacgagcg gtaaccgctt
     1321 tcggcagtac ggattcacgg taagccgcat cctgcttgtc gaatgcatcg gtagacggca
     1381 tggaaaccac gcgcgctttc acgccttcgg cagtcagttt ttcgtaggca gcaaccgcca
     1441 gttcaacttc agaaccggtc gcgatgaaga tcagttccgg ctgaccggca cagtctttca
     1501 gcacataacc accgcgcgcg atgtttgcca gctgctcttc agtacgttcc tgctgcgcca
     1561 ggttctgacg ggagaggata agcgcagtcg ggccgtcctg acgctcaacg ccgtatttcc
     1621 acgcgaccgc ggattcaacc tggtcacatg gacgccatgt ggacatgttc ggggtcacgc
     1681 gcagtgaagc gacctgctct accggctggt gagtcgggcc atcttcgccc agaccgatgg
     1741 agtcgtgggt gtaaaccatc acctgacgct gtttcatcag cgcagccata cgtacggcgt
     1801 tacgtgcgta ttccacgaac atcaggaagg tggaggtgta cggcaggaaa ccaccgtgca
     1861 gggagatacc gttagcaatc gcggtcatac cgaactcgcg aacaccgtag tggatgtagt
     1921 tacccgcagc atcttcgttg attgctttag aaccagacca cagggtcagg ttagacggtg
     1981 ccaggtcagc agagccgccg aggaattccg gcaacagcgg accgaacgca tcaatcgcat
     2041 tctgagacgc tttacggctg gcgattttcg ccggattagc ctgcagttta gcgatgaact
     2101 ctttcgcttt ggcgtcgaag tcagacggca tttcgccttt catacggcgg gtaaattcag
     2161 cggcttcctg cggataagct ttcgcgtaag cagcgaattt ctcgttccat gcggattctt
     2221 tcgcctggcc tgcttctttc gcatcccact gagcatagat ttcagacggg atttcgaacg
     2281 gtgcatattt ccagcccagc tgttcgcggg tcagggcgat ttcagcatcg cccagcggcg
     2341 caccgtggga gtcgtgcgta ccggctttgt tcggggaacc gaaaccgatg atggttttgc
     2401 acatcagcag ggacggtttg tcagtcactg cgcgcgcttc ttctactgcg cgtttgatgg
     2461 atgccgcgtc atgaccgtcg atgtcgcgaa taacgtgcca gccgtaagct tcgaaacgca
     2521 ttgcggtgtc gtcggtgaac cagccttcaa cgtgaccatc gatagagata ccgttgtcat
     2581 cgtagaacgc aatcagttta cccagcttca gcgtacccgc cagagaacaa acttcgtggg
     2641 agatgccttc catcatgcag ccgtcgccca tgaaggcgta ggtgtagtgg tcgacgatgt
     2701 cgtggcccgg acggttaaac tgcgccgcca gcgttttttc tgcaatcgcc ataccgactg
     2761 cgttggcaat accctgaccc agcggacctg tagtggtttc cacaccagcg gtgtaaccca
     2821 cttccgggtg gcccggagtt ttagagtgca gctgacggaa gtttttcagt tcttccatcg
     2881 gcagatcgta accggtgagg tgcagcaggc tgtagatcag catggagccg tggccgttgg
     2941 acagcacgaa gcggtcacgg tcagcccagg acggattctg cgggttgtgt ttcaggaaat
     3001 cacgccacag gacttcggca atgtcagcca tacccatagg ggcacccggg tgaccggatt
     3061 tggctttctg tactgcgtcc atgctcagcg cacgaatagc attggcaagc tctttacgtg
     3121 aggacatttt gactccagat cggatgatga agggcacgcc cttaacgact tgacgacagc
     3181 gcgttttggg ctacgccgga aaatttgcca acaatttacc gcaagccgcg cgtcatgtac
     3241 atggaacatc cttttgccgc ttcagaaatc tctggatcat gctcgcatgt tgcgcaatct
     3301 actcgcccgt ccgctgcgct tttccttata ctgagactaa gcgtcgattc acctgcaaac
     3361 ggcgcatttt tagaataatc ctgaccttgt gcggaagaga aaacatgaaa attcgcgcct
     3421 tattggtagc aatgagcgtg gcaacggtac tgaccggttg ccagaatatg gactccaacg
     3481 gactgctctc atcaggagcg gaagcttttc aggcttacag tttgagtgat gcgcaggtga
     3541 aaaccctgag cgatcaggca tgtcaggaga tggacagcaa ggcgacgatt gcgccagcca
     3601 atagcgaata cgctaaacgt ctgacaacta ttgccaatgc gctaggcaac aatatcaacg
     3661 gtcagccggt gaattacaaa gtgtatatgg cgaaggatgt gaacgccttt gcaatggcta
     3721 acggttgtat ccgcgtctat agcgggctga tggatatgat gacggataac gaagtcgaag
     3781 cggtgatcgg tcacgagatg gggcacgtgg cgttaggcca tgtgaagaaa gggatgcagg
     3841 tggcacttgg tacaaatgcc gtgcgagtag ctgcggcctc tgcgggcggg attgtcggca
     3901 gtttatctca atcacaactc ggtgatctgg gcgagaaatt agttaattcg caattctccc
     3961 agcgccagga agcagaagcc gatgattatt cttacgatct tctgcgccaa cgcggcatca
     4021 gcccggcagg tcttgccacc agctttgaaa aactggcgaa gctggaagaa ggccgtcaaa
     4081 gctcaatgtt tgacgaccat ccagcatccg ccgaacgcgc ccagcatatt cgcgatcgta
     4141 tgagcgcgga tggagttaag taagcatttt attacgccgg atttgacgca taacgcatct
     4201 tatccggcgc ttactcgccc tttttcgctg cctgaatata cagcatctcc agcgccagcg
     4261 tcgccgcagc cagagcggtg atttccgact gatcgtatgc cggagccact tccactacgt
     4321 ccatcccaac aatgttgaga tctttcaggc cgcgtaccag tttaatggcg cgatcagagg
     4381 tcaggccgcc aatcaccggc gtaccagttc ccggcgcgaa agcaggatcc agacagtcga
     4441 tatcaaaagt caggtaaacc ggcatatcac ctacaatctg tttcacctga gcgataacgt
     4501 catccacgct gcgatcgttc acctggcagg cgtccagcac ggtaaagccg ttgtctttat
     4561 cgaactcagt acgaatacca atctgcacgg aatgattcgg gtcgatcaga ccttctttcg
     4621 gcgcggtata gaacatggtc ccgtggtcaa attcacaacc gttcgcatag gtgtcggtgt
     4681 gggcgtcaaa gtgtaccagc gccattttgc cgaaatgctt cgcatgagca cgcagcagcg
     4741 gcagcgtaac aaagtggtca ccaccgaagg agagcatacg cttaccggca gccagcagct
     4801 tctcggcgtg cgcctgcagc ttctcgctca tctcacgggc atctccgaag gcatatacca
     4861 gatcgccgca gtccacgacg ttcaggcgct cacgcatgtc gaaattccac gggaagcgat
     4921 tgtgttccca ggccagattc gtcgaaacct gacggatcgc tgccggaccg tggcgtcccc
     4981 ccgcacgacc agaagtggcc atatcgaacg gcacgccagt aatcacccag tcagcatcgc
     5041 tgtcatatgg ctggaagttc atcggcaggc gtaaaaaacc aaaggcgttg gaaaccagtg
     5101 agttatcgta ttgatgacct aaggtgctca tggatggact cctattacaa agtcgatata
     5161 aaaaaaccct tccgcgtcgt taggcccgac gaggaagggt tggatttgcg aaacacaagt
     5221 ttgcgctaat tatcgcctta aatggacgag gcatcaagcg aggttgctat ttccgacacg
     5281 gtggcggcgc atagccattg ttatctgccg gaaaggcttg tccagagcac ggtggcatcg
     5341 cgttttgcgc cgggccttgt aattcaaagg cggcaaagcc gattaccccg gtattacgca
     5401 catcgccttg cgccgaattg gctgcgtagg aatcagcaac gttggcgaac tgaaaagccg
     5461 cttcggtatg tttatctttg cggaatcctt taatcgctaa cgaatctccg gcattcacaa
     5521 tatagccttt gttattcagc gaaccttgct tgccgttcag cacatccagg ccatcaaccg
     5581 tagcaacgat ctcgtagttg gtgttacgac tgtaattgcg gacatataat tgataattca
     5641 ttccgactct ggcatggaca atatagcgtg gttcattatt tgacgaatca taaacccgcg
     5701 taataggtag agaattaaaa ttaccatcgc gaatagcata ttcaagttcg ttaatacgaa
     5761 tgctataaac gcggtcgtag cccgaaggaa cagtagtcga ataactaata acgatcatct
     5821 ctgccggatc gcgccagact cgttcagcag atacgccctg aaccgatgag tgtacatccc
     5881 caccccaggc ggtgccgaca ttgcctgatg cactgctatt atactgcgca ttactggaac
     5941 aaccggccag cagcacggct aatgctatca acgcgctacg tactttccat tttttcataa
     6001 ccgttccctc tgcatttaat taatgcagcg attgtaggga gattatttaa taagaaaagc
     6061 agctaaatta tttttaagaa tatataaaaa gaaagcgggc cgatttacgt aaataaatcg
     6121 acccgccagc ggaataatct taattattca tcttcaagat aagtataacc gtacaaacct
     6181 gcctcgaact cttcaaggaa ctgttgttgc agttcagcat caagatcggt tttcttcact
     6241 tgatcgcgga actgggttaa cagcgttttc ggatcgagct gtacatattg cagcatgtcc
     6301 gccacggtat cgccttcgtc agacagttct acttctacgc taccgtcagg gaacacgaac
     6361 acgtcaaccg cttcggtatc accgaacagg ttgtgcatat tgccgaggat ctcctgatat
     6421 gcgccgacca taaagaaacc gagcatcggc ggattctctg gatcgtactc cggcattggc
     6481 attgtcgtgg caataccatc accatcaata tagtggtcga tagcaccgtc agagtcacag
     6541 gtaatatcca gcagcacagc gcgacgttcc ggcacttgat ccaacccttc cagcggcaga
     6601 accgggaaca actggtcgat cccccacgca tccggcatcg actggaacag cgagaagttt
     6661 acgtacattt tgtcagccat acgttcctgc aattcatcga taatcggacg atgcgcacgg
     6721 ttttgcggat ccagttgctt ctgcacttca tggcacatgc tcagatagag ttgttctgcc
     6781 catgcacgtt cttgcaggct aaaggtgccg gaagagtagc cgatatgaat atcgtgcaga
     6841 tccatctggc tgtcgtgtaa ccattcacgc agagaacggc gagttcccgg ttcgtgcatc
     6901 tcctgccagg tttcccacat gctttgcagg gcgcgcggtg cgtcttctgc aggtgcggtc
     6961 ggcaccgtgt attcgttacg ttccacgccg atgatatttg acaccagcac ggtgtgatgc
     7021 gcagtcaccg cacgacccga ctcggtgatt accgtcggat gcggcagacc attttcttca
     7081 cacgcatcac caatcgccca gatgatgttg ttagcgtatt cattgaggcc gtagttcacc
     7141 gaacagtcgg actgcgaacg ggtgccttca tagtccacgc ccagaccgcc gccgacgtcg
     7201 aagcactgaa tattgacgcc cagcttgtgc agctcaacat agaaacgcgc ggattcacga
     7261 acgcctgtcg cgatatcgcg aatattcgcc atctgcgaac cgaggtggaa gtgcagcagt
     7321 tgcaggctgt cgagacgccc ggcttcacgc agggtttcaa ccagttgcag tacctgagtc
     7381 gccgccaggc cgaacttcga tttttccccg ccggaggact gccatttacc ggagccctgc
     7441 gaagccagac gtgcacgcac gcccagacga ggaacgacat tcagacgttc tgcttcatcc
     7501 agcacaatgg caatttctga catcttctca atgaccagat agaccttgtg gcccatcttc
     7561 tcgccaatta atgccaggcg gatatattcg cggtccttat aaccgttgca gacgatgacg
     7621 ctacgggtca tgccagcatg tgccagcacc gccatcaact ccgctttgga accggcttcc
     7681 aggcccagcg gttcgcccga atgaatcagg gactcaatca cgcggcggtg ctggttaact
     7741 ttgatcggat aaacgaggaa gtaatcgccg ttatagccgt aggattcccg cgcacgtttg
     7801 aacgcggcgt taatagaacg caaacggtgc tgcaggatct gtgggaaaca gaacagtgca
     7861 ggcagacgct ggccctgtgc ttcacgagtt ttcactaact gcgcgagatc gacgcgagct
     7921 tccgggacgt ccgggtccgg gcacacgcta atgtggccca gctcgttaac gtcatagtag
     7981 ttattgcccc accaggcaat attgtaagta cgcagcatct tgctggcttc ctgggagctc
     8041 attgcaacct cctgcatgga gcgtagtaca ccgtgttcgc ccgctgacga aggcaaaccc
     8101 atagacatgt cgtcagacat agcgaacctc aaattatttt attaagtgta aaacagttaa
     8161 cgactatcgc agcctggaac tgcgataaca acccataagc acatggattt tccagcagtg
     8221 aatgctgacg ctccaactgc gcaaccggtt tctttttcat aacattatta agcacataac
     8281 cgaacgtaag tgtgacagtt cggcgaaacc acgagaaaac tcttgttttt acaagagcgc
     8341 ccttgttcag tcctcagtaa ctgtaaccag ctcttgaatc ctgagaagcg ccgagatggg
     8401 tataacatcg gcaggtatgc aaagcagaga tgcagagtgc ggggaacgaa tcttcaccag
     8461 aacggtgaga caggttaagc agcagacaac ggttcattat ttcgtatcac ctccacggcc
     8521 gcctgttaag acgaacccac aagccaaaac tctgatttca acccggctgg aagtggcaac
     8581 acgaaagaaa cgtcgtgtgc tttttattta agccgcgcgc cgcgttttat accccacaat
     8641 ggcagaaaat tgcaaaagat aaatacgcag aatgccagca ttgtcaggaa aaatttccag
     8701 ccacgttttt aacagaatga gacacgattc aaaaaaaagt ggaaatcggg tgaagaattg
     8761 acctaaaata gccatccaga tgttaatcca tccataccga ttaacactca gactgccagt
     8821 gtttttaacc tgcagagtcg tggtaggatc cgctaccaca gaaaatccac acaacagttt
     8881 gagctaacca aattctcttt aggtgatatt aaatatggca aaacaccttt ttacatccga
     8941 gtccgtctct gaagggcatc ctgacaaaat tgctgaccaa atctctgatg ccgttttaga
     9001 cgcgatcctc gaacaggatc cgaaagcacg cgttgcttgc gaaacctacg taaaaaccgg
     9061 catggtttta gttggcggcg aaatcaccac cagcgcctgg gtagacatcg aagagatcac
     9121 ccgtaacacc gttcgcgaaa ttggctatgt gcattccgac atgggctttg acgctaattc
     9181 ctgtgcggtt ctgagcgcta tcggcaaaca gtctcctgac atcaaccagg gcgttgaccg
     9241 tgccgatccg ctggaacagg gcgcgggtga ccagggtctg atgtttggct acgcaaccaa
     9301 tgaaaccgac gtgctgatgc cagcacctat cacctatgca caccgtctgg tacagcgtca
     9361 ggctgaagtg cgtaaaaacg gcactctgcc gtggctgcgc ccggacgcga aaagccaggt
     9421 gactttccag tatgacgacg gcaaaatcgt tggcattgat gctgtcgtgc tttccactca
     9481 gcactctgaa gagatcgacc agaaatcgct gcaagaagcg gtaatggaag agatcatcaa
     9541 gccgattctg cccgctgaat ggctgacttc tgccaccaaa ttcttcatca acccgaccgg
     9601 tcgtttcgtt atcggtggcc cgatgggtga ctgcggtctg actggtcgta aaattatcgt
     9661 tgatacctac ggcggcatgg cgcgtcacgg tggcggtgca ttctctggta aagatccatc
     9721 aaaagtggac cgttccgcag cctacgctgc acgttatgtc gcgaaaaaca tcgttgctgc
     9781 tggcctggcc gatcgttgtg aaattcaggt ttcctacgca atcggcgtgg ctgaaccgac
     9841 ctctatcatg gtggaaactt tcggtactga gaaagtgcct tctgaacaac tgactctgct
     9901 ggtacgtgag ttcttcgacc tgcgcccata cggtctgatt cagatgctgg atctgctgca
     9961 cccgatctac aaagaaaccg cagcatacgg tcactttggt cgtgaacatt tcccgtggga
    10021 aaaaaccgac aaagcgcagc tgctgcgcga tgctgccggt ctgaagtaat ttttcttcac
    10081 ctgctttcaa aggccagcct cgcgctggcc tttttctttt acctcaccgc ggtggatagg
    10141 cgttcacgcc gcatccggca aaaagctgcc cgcacaataa catcattctt cctgaacacg
    10201 ttttaccgca aattatcatc gctactgaaa ccgattacac caaccacgcc agacaaagat
    10261 ttgtaa
//
LOCUS       DACBVI010000158        10206 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.159, whole genome shotgun sequence.
ACCESSION   DACBVI010000158 DACBVI010000000
VERSION     DACBVI010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.159"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(103..258)
                     /gene="hokD"
                     /locus_tag="IU325_19075"
     CDS             complement(103..258)
                     /gene="hokD"
                     /locus_tag="IU325_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HAP5811108.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TVYEPEE"
     gene            359..541
                     /locus_tag="IU325_19080"
     CDS             359..541
                     /locus_tag="IU325_19080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811109.1"
                     /translation="MLPGAFLYLPLVFMPEADSLKHPQSFYLTKISPQTVFVQHNKYP
                     TKPMEFAVFTASIQCT"
     gene            complement(516..734)
                     /locus_tag="IU325_19085"
     CDS             complement(516..734)
                     /locus_tag="IU325_19085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811110.1"
                     /translation="MFKCNCALIVGGIMKNALQFLFVAFWLFVSCMPIIFTARYMEKV
                     DVLILIFGYINALFLGVFMAVMCIEYWR"
     gene            complement(1405..1587)
                     /locus_tag="IU325_19090"
     CDS             complement(1405..1587)
                     /locus_tag="IU325_19090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811111.1"
                     /translation="MRKFKIIIETGIAGGDFEDEFEVDDDATPDEIHDEAKDIFFNYC
                     NYSYHEIKDEEEEQNG"
     gene            complement(1681..2094)
                     /locus_tag="IU325_19095"
     CDS             complement(1681..2094)
                     /locus_tag="IU325_19095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811112.1"
                     /translation="MKLAIRLQLPLAFASKERSMEIKPEDELSNIVLFPVKEDDPRNQ
                     VNFLYEPSERPYCHHASVRVDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRL
                     LRQEERERRKNIEKLIQIERNAKARIRRATKSRTE"
     gene            complement(2095..2517)
                     /locus_tag="IU325_19100"
     CDS             complement(2095..2517)
                     /locus_tag="IU325_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HAP5811113.1"
                     /translation="MAKVFTQEEREKIKGQVLELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPVVVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRKSEAMQRVLAFYQGNFQEAVL"
     gene            complement(2558..3628)
                     /locus_tag="IU325_19105"
     CDS             complement(2558..3628)
                     /locus_tag="IU325_19105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HAP5811114.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGESRNVTHMSRRVTTDKDTDKDTDQEDQN
                     TMVHGVKNATNQAGDVQTVTPGQPAGTTPEADSAYALKADSGAVQQVMTARQEQSHQL
                     QQPEADSAIQREADRVVPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWK
                     ARLREGVPPEAMLDGVRRYARYLAATGKTGTEFVQRATTFFGPDRNFENPWLLPVSGT
                     NNQRCVNHISEPDNEIPPGFRG"
     gene            complement(3700..4125)
                     /locus_tag="IU325_19110"
     CDS             complement(3700..4125)
                     /locus_tag="IU325_19110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HAP5811115.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(4109..4351)
                     /locus_tag="IU325_19115"
     CDS             complement(4109..4351)
                     /locus_tag="IU325_19115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811116.1"
                     /translation="MKKSEVLGYFGGVVKTAAALGTSKTTVSMWGEEVPWKWALLIQA
                     VTAGALKYELHIPTVVIPGSDHNPPSNQGGIHENQA"
     gene            4743..5081
                     /locus_tag="IU325_19120"
     CDS             4743..5081
                     /locus_tag="IU325_19120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S24"
                     /protein_id="HAP5811117.1"
                     /translation="MKNSSITSCVQLVGEIPVNTFAVVLESDSMSTSGGGVSIPNGST
                     VFVDPDRTVQPGNIVLALPKGTTTPVIRKLEIEGPDILLVPTNPRYPSIMLDDLSCIL
                     GVCFKIQQDI"
     gene            5374..5526
                     /locus_tag="IU325_19125"
     CDS             5374..5526
                     /locus_tag="IU325_19125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 domain-containing protein"
                     /protein_id="HAP5811118.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTETGARRW
                     LARNTC"
     gene            5948..6169
                     /gene="kilR"
                     /locus_tag="IU325_19130"
     CDS             5948..6169
                     /gene="kilR"
                     /locus_tag="IU325_19130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HAP5811119.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     NLTTKTCITDRMIKVFLGRDGLPVKAESW"
     gene            6169..6339
                     /locus_tag="IU325_19135"
     CDS             6169..6339
                     /locus_tag="IU325_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811120.1"
                     /translation="MTKKIKCAYHLCKKDVEESKAIERMLHFMHGTLSKDEPRKYCSE
                     ACAEKDQMAHEL"
     gene            6414..6689
                     /locus_tag="IU325_19140"
     CDS             6414..6689
                     /locus_tag="IU325_19140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811121.1"
                     /translation="MITNYETTVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLDEGVTKMSLVHIGKNLPAEKKAGFLATLIAMKLNGEI"
     gene            6789..9389
                     /gene="recE"
                     /locus_tag="IU325_19145"
     CDS             6789..9389
                     /gene="recE"
                     /locus_tag="IU325_19145"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HAP5811122.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASNDFESTCATLDYLIVKSG
                     KKLSSYFKAVATNFPVVNDLPAEGEIDFTWSERYQLSKDSMTWELKPGAAPDNAHYQG
                     NTNVNGEDMTEIEENMLLPISGQELPIRWLAQHGSEKPVTHVSRDGLQALHIARAEEL
                     PAVTALAVSHKTSLLDPLEIRDLHKLVRDTDKVFPNPGNSNLGLITAFFEAYLNADYT
                     DRGLLTKEWMKGNRVSHITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARS
                     MDLDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIG
                     IEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKPQP
                     SGTTAVEQGEAETMEPDATEHHQDTQPLDAQSQVNSVDAKYQELRAELHEARKNIPSK
                     NPVDADKLLAASRGEFVDGISDPNDPKWVKGIQTRDCVYQNQPETEKTRPDMNQPEPV
                     VQQEPEIACNACGQTGGDNCPDCGAVMGDATYQETFDEESQVEAKENDPEEMEGAEHP
                     HNENAGSDPHRDCSDETGEVADPVIVEDIEPGIYYGISNENYHAGPGVSKSQLDDIAD
                     TPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEEFSNRFIVAPEFNRRTNAGKEEE
                     KAFLMECASTGKTVITAEEGRKIELMYQSVMALPLGQWLVESAGHAESSIYWEDPETG
                     ILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQDAFYSDGYEAQFGVQPT
                     FVFLVASTTIECGRYPVEIFMMGEEAKLAGQQEYHRNLRTLSDCLNTDEWPAIKTLSL
                     PRWAKEYAND"
     gene            9382..10191
                     /gene="recT"
                     /locus_tag="IU325_19150"
     CDS             9382..10191
                     /gene="recT"
                     /locus_tag="IU325_19150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HAP5811123.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAVKNSDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFSFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSLSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
BASE COUNT         2749 a         2534 c         2269 g         2654 t
ORIGIN      
        1 atttacagat aaaataaacc cgccgaagcg ggttaagtgc gggtgcattg aggatgcctg
       61 acacatcaga ggtggcgagg gatttctccc tcgccgggtc tcttactcct caggttcgta
      121 aactgtgaag acagcgacct ccgtctggcc ggttcggatt cgtacctcgc agaggtcttt
      181 cctcgttacc agtgccgtca ctatgacggt taaacagatg acgatcaggg cgattaacat
      241 cgccttttgc tgcttcatag cctgcttctc cttgaccttt cggtccgtaa gaggctaatc
      301 tctatgtgtc gcatagatat ggcctcagat taatgttaaa cgtcttgcag gacgcgtaat
      361 gttacctggg gcttttctct atctgccgtt ggtgttcatg cccgaggcag atagcctcaa
      421 gcacccgcag tcattctact taactaagat ttccccgcaa accgtttttg tccagcacaa
      481 taaatatcca actaaaccaa tggagttcgc tgtatttacc gccagtattc aatgcacatg
      541 accgccatga acacccctaa aaaaagggca tttatatatc caaatattaa tatcaaaaca
      601 tcaacttttt ccatatacct tgctgtgaag atgatgggca tacatgatac gaacaaccag
      661 aacgcaacaa acaaaaactg caatgcgttt ttcattattc ctcctacaat caatgcgcaa
      721 ttacatttaa acacacctca atttggccgg acatataaat atctaaacta gaaaaaatca
      781 cttacatagc gttacaaact ctttagtcta aaggttcatc gtaaaacatt ccccatactt
      841 atcagtccgt tccgtgccag gtagcttatt gccttatctg gcaacctata atcaggtttc
      901 cgctttttca gttggctggt cgtttaaccg acatagttaa cccattaatc tagttgccgg
      961 atgttggtgg attttcgcgt tttagttgtt cataaaagtg cacagcttta accagttctt
     1021 ctgatgtaac cgggactggc ggggcagtga ataaggcctg aatttcatag ttcggactgt
     1081 cgttacaatc ctcttttttc ggtacatatt tccagtcacc agaccactac ttcccctgaa
     1141 agtccgtaac gccttttttt tcacgtagcg atatcgccat gccactgttt ttgcttgccc
     1201 cgccgtttca tgcccttcct gataattaac ctcgctcatt catcgcccca ctcatcacaa
     1261 tatgcttcga ccggagtttt tcctgcttca taatcatcac gccatgcttc agcatcagca
     1321 gcactgccac cacgtaactc tgcatagtcc attaacagtt catgccatgc ttcaaaactg
     1381 acgttgtatt tagttgaacc aaaatcagcc attttgttct tcctcttcgt cttttatttc
     1441 gtgatatgag taattgcagt agttaaagaa aatatctttt gcttcgtcat gtatttcatc
     1501 aggcgtcgca tcatcatcca cttcgaattc atcctcgaaa tctccaccgg ctattcccgt
     1561 ttcaataatt attttaaact ttcgcatttc actaccgccc tttcgggcgg cctcctgatg
     1621 ttctgagggt gcagaaatcc ctccggttaa ggattaaatt tttaacagag ctaaatttaa
     1681 ttattcagtt ctggattttg tcgccctgcg tatccgcgct ttcgcgttac gctcaatctg
     1741 aattagcttt tctatatttt ttcgcctttc ccgctcctcc tgacgcaaga gccttacatc
     1801 atctgccagt ctggtttctc ttttcgccac agagagcatc cagtcaaatg gctccacaac
     1861 tgcaccgcag attttacagc ggacctgacg ctctttttcg tcaacccgga cagaggcgtg
     1921 atgacaatat ggtctttccg atggctcata aagaaaatta acctgattac gagggtcatc
     1981 ctcttttacc ggaaataaaa cgatattgct taactcatcc tctggtttta tttccatgct
     2041 cctctccttt gatgcgaatg ccagcggtaa ttgaagcctg atagctaatt tcactcacag
     2101 taccgcctcc tgaaaattac cctgatagaa agccagtaca cgctgcatag cttcactctt
     2161 ccggcactcg cgacagatta tgtttaggcg actgtcgtag cgacgtattt ctccgtcagg
     2221 taacgaccag ataaggtccg gatcaaccac aaccggtttc ttcagctttg ccctcgataa
     2281 ttttttgcgg gcattttgcc agtctttacg agcctgttca gacgggaata acccgtagcc
     2341 agaattgtat acatcgccac tggcaaccag ctctctggcc agaacgctca tcagatatct
     2401 tgttgcccca gttttagctt ccagttgtcg taacgtctcg cgcccactct ggcgtacgag
     2461 ttcaagaacc tgtcctttaa ttttttcccg ctcttcttgt gtaaaaactt ttgccacaag
     2521 ccctcctgaa aattacctca tgaccagaaa ttaacactta ccccctgaag cccggcggaa
     2581 tttcgttatc cggttcagaa atatgattca cacaacgctg gttgttcgtg ccgcttaccg
     2641 ggagcagcca ggggttttca aaattccggt ccggtccaaa aaacgtcgtc gctcgctgaa
     2701 caaattccgt tcccgttttc ccggtagccg caaggtatct tgcgtaacgc ctcacgccat
     2761 ccagcatggc ctctggtggc accccctcgc gtaatctggc tttccaggca ctgaaagcgg
     2821 atttcttcgg gtttgccccg gcacgcaacg ggtattcccg ccagacctgt tcgaacacat
     2881 caggataatc cactcgtccc acaggctgcc cggtgttttc cgggactacc cgatcggctt
     2941 cccgctgaat ggcggaatcg gcttcaggct gctgaagttg gtgtgattgc tcctgccttg
     3001 cggtcatcac ctgctgcaca gcgcccgaat cggctttcag cgcatacgct gaatcggctt
     3061 ccggtgtcgt gcctgcgggc tggccaggag tgacggtctg aacatcccct gcctggttcg
     3121 tggcgttttt tacgccatgg accatagtgt tttgatcttc ttgatctgta tctttatctg
     3181 tatctttatc tgtcgtgact cgtcgtgaca tgtgcgtgac atttcgtgac tcgccgtgac
     3241 aatcgccatt ttgttcccgc tttctttccc tctctcgctg cgccctcttg cgctctgccg
     3301 gagattttgc ggtttgcgaa atattgccgt tgtcctcttt cagcacctgg cgtttttccc
     3361 atccagtgat taaatcacca tcaagtaccc gcccctgcat cgtctgcaaa attgaatcaa
     3421 ttacctcttc tgtcacgtcg agcgcacttg ccaaatcttc tgtcgtgaca tcaatgtgac
     3481 ctcgcgtgac atttcgtgac gcgctcacca ggaggtggat atacactgcc atcactgttg
     3541 caattggctg ccctgacacc ctggcaattg ttcgccactt agggtcattt ggcatgtcat
     3601 gccataatct gagccaggcg ttagccatac tcacctcttc tgataccgaa tctttttact
     3661 cacgagttgc cggaagcgat tcgatatggc tattgtcagt caatgtactg ccacagcatt
     3721 tcctgccggg ccaccacggt tcatctgatt gaaaccggcg attgccactg cgacaaaatc
     3781 atcagcgtct ctcaccagtc gctcccgcgt ctccaccagc tcccgaaaat aagctgaact
     3841 gtggctgcgc attctggcca ccagcaaagg tggcattgcc ttttcgatcg ctggtaacaa
     3901 cgcctgaatt ttttcaactg catcaggggt gtctttctct acccagcgga aaattttctg
     3961 ggtattgcga gccagggctt ccggatggct gtcgtcatac agttccggga acgtcattcc
     4021 cagctcgaaa tacgctttgg taattttcgc agccggtact ttttcgccgt ccggatgcgc
     4081 ccaggcattc atcgccatgc ggatgtgttc atgcttgatt ttcatgaatc cccccttggt
     4141 tagaaggcgg attatgatca gaaccgggaa tgacaaccgt cggtatgtgt aactcatatt
     4201 tgagcgcccc ggcagtgact gcctgaatta gcaacgccca tttccacgga acctcttccc
     4261 cccacatgct gactgtggtt tttgacgttc ctagagctgc ggctgtttta acaactccgc
     4321 caaaatagcc taatacttct gattttttca tgagtcgctc cataaaactg aacgtcaaaa
     4381 gtttaataat caaaaccaaa gaaagtcaag aaacaaaacc atctgtgttt taaaatcaaa
     4441 acatgagcaa gcaaactata tctgaacgca taacccaacg tatgcatgcg ctaaacctga
     4501 aaggcaaaga ccttgtcaat ggcactggcg catcaaaagg ctccgtaagt caatggatga
     4561 acggtggagg agcgccgtcc tcgcgttaca taagttcact ggcaaaaata ttgaaagtaa
     4621 acgaaaattg gcttcttaat ggaggagagt taaatacagg tgattcgctt gatctatctt
     4681 taccgccgat aaaaacggtt ccgctactat cacttcagca ggcagcaagc tggagtgatt
     4741 atatgaaaaa ttcctcaata acctcttgtg tgcagcttgt cggagaaatc ccggtcaata
     4801 cctttgcagt tgttctagag agtgacagta tgtcaacatc tggtggggga gtttccatcc
     4861 caaatggttc aacagttttt gttgatcccg atcgaaccgt acaaccagga aatattgtcc
     4921 ttgccttacc caaagggacc acaacacctg tcattcgtaa actggagata gaagggccgg
     4981 atattctttt agtccccacg aatcctcgct acccttcaat tatgctggat gatctatctt
     5041 gcatattggg cgtatgcttt aaaattcaac aagatattta accaacctca tctatttgat
     5101 taactgtatg ccatcgtggt gatggcttaa cagctgcctg cttaaaatgt tttgataaaa
     5161 aaacattgac ctgaaaagtt cgtttttcta aacttcattc attccctcac cccatcctac
     5221 agaatgcagg gcaatacttc gagttaccag gcagtggtca ggggttaagt agccagcccg
     5281 aggcgtaaga acatgacggc agggttcaac tttaataact atgcagcagg tttttgttcc
     5341 gctaccccag cgttaagggg aaatgaggtc agcatggata ctatcgatct tggcaacaac
     5401 gaatctctgg tatgtggcgt gttccccaac caggatggaa cgttcaccgc catgacttat
     5461 accaaaagca aaacattcaa aactgaaacg ggcgcacgtc gctggcttgc cagaaatact
     5521 tgctaatcca ttatttagat taattcaata ttctcgctgt aggggtatag cagaaaccac
     5581 caaagcccgg aggtggtgaa ataaaaccgg gcacaatacg aaggcgcatt tccggtatcc
     5641 ataaagagtc ggtcttgtct gttaaattta aatggtggga gtgcgcctcc ggttgtaaat
     5701 aacgacattg ctgtgtgtag tcctggcgtc atcagttttt ttcttgaagt tcggctgatg
     5761 tccgcccttt ttaaagtgaa ttttgtgatg cggtgaatgc ggctaagcgc acgcggcaca
     5821 gttaaaagtc atgttagtcc ttattggttt gggtgggaaa gccgactgta attgttaact
     5881 ggttgcagtc acctggaggc accaggcacc gcatcgacaa agttcatttg taaaaatgga
     5941 gataattatg attgctcatc actttggaac tgatgaaata ccacgtcagt gtgtgactcc
     6001 tggcgattat gttcttcatg aaggccggac atatattgcc tcggcaaaca atattaaaaa
     6061 gcgaaaactt tatattcgta acctgaccac aaaaacatgc attactgacc gcatgattaa
     6121 agtcttcctc ggtcgtgatg gtttacctgt aaaggcggag tcatggtgat gaccaagaaa
     6181 ataaaatgtg cttaccacct ttgcaaaaaa gacgttgaag aaagcaaagc tattgaaaga
     6241 atgcttcact tcatgcacgg gactttatca aaagacgaac cgagaaaata ttgcagtgaa
     6301 gcttgtgccg aaaaagacca gatggcacac gaactttaat taattgacta ttcgaaactg
     6361 aatttatgcc agaaatggca gggattcgct caaccttaat taaggagaaa aacatgatta
     6421 ccaattatga aaccactgtt gtaactaccg atgacattgt tcatgaggtg aatctggaag
     6481 gaaagcgcat tggctacgta attaaaacag aaaataaaga aaccccattc actgtggttg
     6541 atatcgatgg tccatcaggc aacgtaaaaa cacttgatga aggtgtcaca aaaatgagcc
     6601 tggttcacat cggaaagaat ctgcccgcag aaaaaaaagc cggatttctg gcaactctga
     6661 tcgcaatgaa attaaacggt gaaatctgaa aaaaagaaag cctgcactgt gtgcaggcct
     6721 gagtgaagaa cctgggacat ttattcatca ctcgcagtaa ttttaatctg agttgaggtt
     6781 aaaaaacaat gagcacaaaa ccactcttcc ttttacggaa agcgaaaaaa tcatccggtg
     6841 agcctgacgt cgtcctgtgg gcaagcaacg attttgaatc gacctgtgcc actctggact
     6901 acctgatcgt taagtcaggt aaaaaactga gcagctattt taaagctgtt gccacgaatt
     6961 ttcctgtcgt taatgacctg cccgctgaag gtgagatcga ttttacctgg agtgaacgct
     7021 atcaactcag caaagactcc atgacatggg aactaaaacc gggagcagca ccagacaacg
     7081 ctcactatca aggcaatacc aacgtcaacg gcgaagacat gactgagatt gaggagaata
     7141 tgctactccc aatttctggc caggaactgc ccattcgttg gcttgctcaa cacggcagcg
     7201 aaaaaccggt aacgcacgtt tcacgcgacg gactccaggc attacacatt gctcgggctg
     7261 aagaactacc ggctgttact gccctggctg tttcccacaa aaccagcctg ctcgacccgc
     7321 tggaaattcg cgatctccac aaactggttc gtgacactga caaagttttc cctaatcctg
     7381 gtaattcaaa cctgggactg ataactgctt ttttcgaagc atacctgaac gctgactaca
     7441 ccgatcgagg actgctgaca aaagagtgga tgaagggtaa tcgtgtttca cacatcactc
     7501 gcacggcttc cggtgctaat gctggcggcg gaaacctcac cgatcgcggc gaaggtttcg
     7561 tacacgatct gacgtcactg gcgcgcgacg tagccactgg cgtactggcc cgttcaatgg
     7621 atctggacat ctataacctt catccggcac acgctaaacg cattgaggaa attatcgctg
     7681 aaaataaacc gcccttttct gttttccgcg acaaattcat caccatgcct ggcgggctgg
     7741 attattcccg cgccatcgtg gttgcgtccg taaaagaagc accaattggg atcgaggtca
     7801 tccccgcgca cgtcactgaa tatctgaaca aagtactgac tgaaaccgat catgccaacc
     7861 ctgatccgga aatcgtggat attgcctgcg gtcgctcctc tgccccgatg ccgcagcgag
     7921 taacagaaga aggaaaacag gatgatgaag aaaaaccgca accatctgga acaacggcag
     7981 ttgaacaggg agaggctgaa acaatggaac cggacgcaac tgaacatcat caggacacgc
     8041 agccgctgga tgctcagtca caggtaaatt ctgttgatgc gaaatatcag gaactgcggg
     8101 cagaactcca tgaagcccgg aaaaacattc catcaaaaaa tcctgtcgat gccgataaat
     8161 tgcttgctgc atcacgtggt gaatttgttg acggaattag cgacccgaac gatccgaaat
     8221 gggtaaaggg gatccagact cgcgattgtg tgtaccagaa ccagccagaa acggaaaaaa
     8281 ccagaccaga tatgaatcaa cctgagccag tagtgcaaca ggaaccggaa atagcctgca
     8341 atgcctgcgg ccagactggc ggggataact gccctgactg tggtgcggtg atgggcgacg
     8401 caacatacca ggaaacattc gatgaagaga gtcaggttga agctaaggaa aatgatccgg
     8461 aggaaatgga aggcgctgaa catccgcaca atgagaatgc tggcagcgat ccgcatcgcg
     8521 attgcagtga tgaaactggc gaagtcgcag atcccgtaat cgtagaagac atagagccag
     8581 gtatttatta cggaatttcg aatgagaatt accacgcggg tcccggtgtc agtaagtctc
     8641 agctcgatga cattgctgat actccggcac tatatttgtg gcgtaaaaat gcccccgtgg
     8701 acaccacaaa gacaaaaacg ctcgatttag gaaccgcttt ccactgccgg gtacttgaac
     8761 cggaagaatt cagtaaccgc tttatcgtag cacctgaatt taaccgccgt acaaacgccg
     8821 gaaaagaaga agagaaagcg tttctgatgg aatgcgcaag cacaggaaaa acggttatca
     8881 ctgcggaaga aggccggaaa attgaactca tgtatcaaag cgttatggct ttgccgctgg
     8941 ggcaatggct tgttgaaagc gccggacacg ctgaatcatc aatttactgg gaagatcctg
     9001 aaacaggaat tttgtgtcgg tgccgtccgg acaaaattat ccctgaattt cactggatca
     9061 tggacgtgaa aactacggcg gatattcaac gattcaaaac cgcttattac gactaccgct
     9121 atcacgttca ggatgcattc tacagtgacg gttatgaagc acagtttgga gtgcagccaa
     9181 ctttcgtttt tctggttgcc agcacaacta ttgaatgcgg acgttatccg gttgaaattt
     9241 tcatgatggg cgaagaagca aaactggcag gtcaacagga atatcaccgc aatctgcgaa
     9301 ccctgtctga ctgcctgaat accgatgaat ggccagctat taagacatta tcactgcccc
     9361 gctgggctaa ggaatatgca aatgactaag caaccaccaa tcgcaaaagc cgatctgcaa
     9421 aaaactcagg gaaaccgtgc accagcagca gttaaaaata gcgacgtgat tagttttatt
     9481 aaccagccat caatgaaaga gcaactggca gcagctcttc cacgccatat gacggctgaa
     9541 cgtatgatcc gtatcgccac cacagaaatt cgtaaagttc cggcgttagg aaactgtgac
     9601 actatgagtt ttgtcagtgc gatcgtacag tgttcacagc tcggacttga gccaggtagc
     9661 gccctcggtc atgcatattt actgcctttt ggtaataaaa acgaaaagag cggtaaaaag
     9721 aacgttcagc taatcattgg ctatcgcggc atgattgatc tggctcgccg ttctggtcaa
     9781 atcgccagcc tgtcagcccg tgttgtccgt gaaggtgacg agtttagctt cgaatttggc
     9841 cttgatgaaa agttaataca ccgcccggga gaaaacgaag atgccccggt tacccacgtc
     9901 tatgctgtcg caagactgaa agacggaggt actcagtttg aagttatgac gcgcaaacag
     9961 attgagctgg tgcgcagcct gagtaaagct ggtaataacg ggccgtgggt aactcactgg
    10021 gaagaaatgg caaagaaaac ggctattcgt cgcctgttca aatatctgcc cgtatcaatt
    10081 gagatccagc gtgcagtatc aatggatgaa aaggaaccac tgacaatcga tcctgcagat
    10141 tcctctgtat taaccgggga atacagtgta atcgataatt cagaggaata attcagcctg
    10201 gcggtg
//
LOCUS       DACBVI010000159         9967 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.160, whole genome shotgun sequence.
ACCESSION   DACBVI010000159 DACBVI010000000
VERSION     DACBVI010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.160"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            4..1218
                     /locus_tag="IU325_19155"
     CDS             4..1218
                     /locus_tag="IU325_19155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HAP5811124.1"
                     /translation="MITASINNVNAGNLTFMVGDDAGVGNLNGPSGDKLISFSYPVGG
                     VNNIVFYNPTSATPTNAQIKLYWSDLDTSSSRGFVYCTSKSNSSGMPITVRHNMVDSG
                     KMADGHRLYETNVTGLYYTLMLNRFWSYELRSSSPDIYVGDDGWHTVQFSITGDYIRG
                     QCNTSRYQAVGGLLHDATVEFYTDSSFNPAPNTNVQLKHTGDYLYSFANEGPGVPNVS
                     ASKMLKIDFNLTDINIQLPTCFTSILLGESVTRSTVNLGEYTVGQITSDSATPVNFQI
                     SLQNCVRISSIKTKLKSNVVGVNNPLLLGNTLTGTDDAKGAGVLIEGLPNSKNSQNFT
                     LKPNDETSSYNDAEDDPGENNGIYNPDYPNANDRTTTQDLKFQATLKRDGNATVKPGG
                     FKATSTFQVTYE"
     gene            1332..2126
                     /gene="panB"
                     /locus_tag="IU325_19160"
     CDS             1332..2126
                     /gene="panB"
                     /locus_tag="IU325_19160"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAP5811125.1"
                     /translation="MKPTTIASLQKCKQEKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATQEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAA
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            2138..2989
                     /gene="panC"
                     /locus_tag="IU325_19165"
     CDS             2138..2989
                     /gene="panC"
                     /locus_tag="IU325_19165"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAP5811126.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSENSKRAVILVAAWLGDARLID
                     NKIVELV"
     gene            complement(3355..4299)
                     /locus_tag="IU325_19170"
                     /pseudo
     CDS             complement(3355..4299)
                     /locus_tag="IU325_19170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            4573..4953
                     /gene="panD"
                     /locus_tag="IU325_19175"
     CDS             4573..4953
                     /gene="panD"
                     /locus_tag="IU325_19175"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAP5811127.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(4957..6186)
                     /locus_tag="IU325_19180"
     CDS             complement(4957..6186)
                     /locus_tag="IU325_19180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP5811128.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVFNAPSSGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYVTLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     DPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAAKEAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(6250..6690)
                     /locus_tag="IU325_19185"
     CDS             complement(6250..6690)
                     /locus_tag="IU325_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP5811129.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWCGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMLV
                     YRETLSSAEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(6795..7565)
                     /locus_tag="IU325_19190"
     CDS             complement(6795..7565)
                     /locus_tag="IU325_19190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP5811130.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(7562..8488)
                     /locus_tag="IU325_19195"
     CDS             complement(7562..8488)
                     /locus_tag="IU325_19195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP5811131.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            8597..9259
                     /locus_tag="IU325_19200"
     CDS             8597..9259
                     /locus_tag="IU325_19200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HAP5811132.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(9300..9836)
                     /locus_tag="IU325_19205"
     CDS             complement(9300..9836)
                     /locus_tag="IU325_19205"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP5811133.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
BASE COUNT         2639 a         2563 c         2287 g         2478 t
ORIGIN      
        1 tggattatca cagccagcat taataatgta aatgctggaa atcttacttt tatggtcggt
       61 gacgatgctg gcgtaggaaa ccttaatggc ccatcaggcg ataaactaat atcattttcc
      121 tatccggttg gtggagtaaa caatattgta ttttataatc caacatcagc cacaccgacc
      181 aatgcacaaa taaaattata ttggagtgac ctggacacat caagtagccg tgggtttgta
      241 tattgtactt caaaaagtaa ttccagcggt atgccaatta ctgtccggca taacatggtt
      301 gactcaggaa aaatggcaga tgggcacaga ttatatgaaa ctaatgtaac cggtctatat
      361 tatacgttaa tgctcaatag attctggtcg tatgaacttc gctcaagctc tccagatata
      421 tacgttggag acgatggctg gcacactgta caattttcta ttacgggtga ttatatacga
      481 ggtcaatgta atacttccag gtatcaagca gtcggaggtc ttttgcacga tgccacggtg
      541 gaattctata ccgacagcag ttttaaccct gctccaaata ctaacgtgca attaaaacat
      601 actggtgatt atttgtattc attcgcaaat gaagggccag gggttccaaa tgtaagtgca
      661 tctaagatgc taaaaattga ttttaattta acagacatca atatccaact gccaacatgc
      721 tttacgtcta tccttttggg tgagagcgta acgcgatcaa ccgtaaatct cggggaatat
      781 actgttggac agatcacctc agattctgca acacctgtta attttcagat ctcgctgcaa
      841 aattgtgttc gtataagcag tattaaaaca aaactaaaat ctaatgtagt aggagtgaat
      901 aatcctctgt tgcttggtaa tactttgaca ggaaccgatg atgcaaaagg tgccggagta
      961 cttattgaag ggttacccaa cagcaaaaat tcacaaaatt tcacgcttaa gccaaatgat
     1021 gagacatcaa gctataacga tgctgaagat gatcctggag aaaacaatgg aatttataac
     1081 ccagattatc ctaatgccaa cgatcgtact acaacccagg acctgaaatt tcaggcaaca
     1141 ttaaaacgcg atggtaatgc aactgtgaaa cccggcggat ttaaggctac cagtacattc
     1201 caggtcacct acgagtaaca ggtttaaccg caccgccatc atctggcggt gctgtaattg
     1261 ataaatcatt gcccccacca atgtgactga taaaatgcca gacagcacga cattacttat
     1321 caggatacgt tatgaaacca accactattg cttcattgca aaaatgcaaa caagaaaaaa
     1381 aacgtttcgc gaccatcacc gcttacgact atagcttcgc aaaactgttt gccgatgaag
     1441 ggcttaacgt catgctggtt ggcgattcac tgggcatgac ggttcagggg catgactcca
     1501 ctctgccagt taccgttgcc gatatcgcct accacactgc tgctgtacgt cgcggtgcgc
     1561 caaactgtct gctgctggct gatctgccgt ttatggcgta tgccacacaa gaacaagcct
     1621 ttgaaaacgc cgcgactgtc atgcgtgccg gtgccaacat ggtcaaaatt gagggcggtg
     1681 agtggctggt agaaaccgta caaatgctga ccgaacgtgc cgttccggtt tgtggtcatt
     1741 taggcctaac cccacagtca gtaaatattt tcggtggcta caaagtccag gggcgcggcg
     1801 atgaagcggc cgatcgactg ctcagcgatg cattagcttt agaagctgct ggggcacaac
     1861 tgctggtact ggagtgcgtg ccggttgaac tggcaaaacg tattaccgaa gcactggcga
     1921 tcccggttat tggcattggc gcaggcaacg tcactgacgg gcaaattctc gtgatgcacg
     1981 acgcattcgg cattaccggc ggtcatattc ctaaattcgc caaaaatttc ctcgccgaaa
     2041 cgggcgacat ccgcgcggct gtgcggcagt atatggctga agtggagtcc ggcgtttatc
     2101 cgggcgaaga acacagtttc cattaaggag tgacgttgtg ttaattatcg aaaccctgcc
     2161 gctgctgcgt cagcaaatcc gccgcctgcg tatggaaggt aagcgcgtgg cgctggtgcc
     2221 caccatgggt aacctgcacg acggccatat gaagctggtc gacgaagcca aagcccgcgc
     2281 cgatgtggtc gtcgtcagta ttttcgttaa cccgatgcag ttcgaccgcc cggaagatct
     2341 ggcacgttat ccacggacct tgcaagagga ctgcgaaaag ctgaacaagc gcaaagtgga
     2401 tttagttttc gccccttcgg taaaagagat ctacccgaac ggtacagaaa cccacaccta
     2461 tgttgatgtt cccggtcttt ctaccatgct ggaaggtgcc agccgtccgg gacattttcg
     2521 cggcgtttcg actatcgtca gcaagctgtt caatctggtc cagccggaca tcgcctgctt
     2581 cggtgaaaaa gattttcagc aactggcgct gatccgcaaa atggttgctg atatgggctt
     2641 tgatattgag attgtcggtg tgccaattat gcgcgccaaa gacggtctgg cgctgagttc
     2701 ccgtaacggt tatctgacgg cggaacaacg caaaattgcg cctggtctgt acaaggtttt
     2761 aagttcgatt gccgacaaat tgcaggctgg cgaacgggat ctcgatgaaa ttattactat
     2821 tgccgggcaa gaactgaatg aaaaaggctt ccgcgccgat gatattcaga ttcgtgatgc
     2881 cgacaccttg ctggaggtct ctgaaaacag caaacgtgca gtgattctgg tagccgcctg
     2941 gcttggcgat gcccgcctga tcgacaacaa aatagtcgag ctggtgtaat acttaactgg
     3001 cgctacggct gatggcgcca gttattcatt gccacgttat ctgtaagcga taattcataa
     3061 cgcagacaac ttagttagag ttaattagct tatcaacctc tgtaagagat aaagttgcca
     3121 tctctgcaac gtcttcccga gacattcctt tactcagaag acgctgtgcc agccttacct
     3181 tccggagtcc ctctttatat cccttctgaa atcccttctg aaatcccttc tcttcaaacc
     3241 actgcgccaa cgtctgcctg gtttctctcc gtgtctttat ttccataggt tcccttaatt
     3301 atctggtcag ttaacaataa caattatctg gctataactg atgatgcaga caacttagtt
     3361 aatcagctta tcaatctcgg caaggggtaa atttgccatc tctgcaacgt cttcccgaga
     3421 cattccttta ctcagaagac gctgggcgaa ttcctgactt acttcctgtg ttacttcctg
     3481 tattacctct tgtcttacct cttgtcttac ctcttgtctt acctcctgtc ttccttcctg
     3541 tcttcccctc tcttcaaacc actgcgccag cgtcatcata gactcccctc ccgtttacct
     3601 gtctctcagc acaccataaa acaaatccgc ttgttcagta tgaccgcgtt gcagcatata
     3661 gttttgcatg gcaactaact gacttccgct agtgtaccct tcgtcaatca gcgtgaccag
     3721 ttgctccagc aataacatta agtcgcgctg gcgaatatgt ttttgcagta gttcgagaat
     3781 cgcaatccgc cgatgctgca tgatttcgtc atccggtgtg atggtgatat ccaccagcgg
     3841 gaaagggttg ttgtaaacgc gtcgcgccag ctccggcgag taaaacatat caaaccagca
     3901 cattgagagc ggataaggtg tggcctcgcc ctgataaaac agaatcggca ccaccagcgg
     3961 cagcttatcg tgatccgcct ccagatgccg gtgcatggca gcaatagaat aacgcatcat
     4021 gcgaaaggcc attttcttat ccggcttgct ttgatgttca atcaccacat gcaaataacc
     4081 gggattaccc tgcatttgca cggaatagag cacgtcagtg ctgtgccctt tcaggctttc
     4141 ttcaataaaa ctccccgact caagatgcag cgtgtcgagg tcacacagtt cgcgtagttc
     4201 cgctggcaaa tggatatcca gaaagtcacg agccgtttcc gcatgcatta aaaattgttt
     4261 aaataccgcg tcatgcggtg tggtactcgg tgcatccatc ttccctgcct tccgtgttgt
     4321 cttgcgtgaa ggcagtatcc agtcaaggcg cgccgtcgtc agccgcctct ttattgttta
     4381 ccgagcagcg ttcagagaaa tttcaattgg ttacaggcgt tacatggcac gcttcggcac
     4441 ggtacgcaaa ctgctgatgc aaatgccgtc agatgagtag acactaaaca aaaatcgggc
     4501 aatactgcgt gagaatttcc tcaaagcagg catcgcctgc ttcgttaacg acagggtaga
     4561 aaggtagaag ttatgattcg cacgatgctg cagggcaaac tccaccgcgt gaaagtgact
     4621 catgcggacc tgcactatga aggttcttgc gccattgacc aggattttct tgacgcagcc
     4681 ggtattctcg aaaacgaagc cattgatatc tggaatgtca ccaacggcaa gcgtttctcc
     4741 acttatgcca tcgcggcaga acgcggttcg agaattattt ccgttaacgg tgcggcggcc
     4801 cactgcgcca gcgtcggcga tattgtcatc atcgccagct tcgttaccat gccagatgaa
     4861 gaagctcgca cctggcggcc caacgtcgcc tattttgaag gcgacaacga aatgaaacgt
     4921 accgcgaaag ccattccggt acaggttgcc tgataatcac ccctgcggct ggttactcac
     4981 cagccgcgac attgtctcca gcgaatccgt tcttaagata taaagtcgtt ttagtaacaa
     5041 cggattatcc cccggtttta ctttgccttt catggttgtc accgccaggt gaaatccggc
     5101 ttcttttgct gccttcacgg ctttgtcatt aaatccgcca aacggatacg aaagatacca
     5161 gacatgcgga ttaaattgcg ccagagcgcg gcgtgaacgt gcaaaatcaa acaggatatt
     5221 gtgctcacta cggctcagta atatgggtcg gcgataacca tccacccgat gcaaaaaatg
     5281 ggtatgtgac tggaaatcaa atacatcgcg aatttcgtta agttcagaaa cgctcataaa
     5341 ttgcagcgat tttgggtccc acttctgcgg gtgacgtttg atgcgcgagg taacaataaa
     5401 cgccgtcgcc ttcatgccat attgtttcaa cacaggatac gcatagcggc tcaccgactt
     5461 taggccatca tcaaaggtaa tcaccaccgc tcgcgcaggg agattgatct tattcttcac
     5521 gtaaccttcc agttgcgcca tgctcagtgt cacgtatccc ctgtcgcgca gccaggccat
     5581 ctggttattg aaagcgcgta ccgaggtggt cgtcgaagta tggcgaaaac gggtattttc
     5641 ttcgtcgcgc agaatatggt gataggttag caccggcagg ccattatcgg gttgggcatc
     5701 cagcgcgctg atataggcca gtcgatcgcc aatacggatc tgataccagg tttgatttaa
     5761 cctgtctttc agtttatgca aaattgggta gcgcaaattg tccgccagta ccccaaacgg
     5821 cgcactgccc gaactcggcg cgttgaagac cggcgtatct ttccaggtta ttaagttctg
     5881 attactcagc ggcttgttga ggtcacccaa accatcttca actttttgtc gcccctgaac
     5941 cggctcaaga tgacctttat cgataaaccc tttaccaaag ccaaaattaa atgcgtaata
     6001 acttgcggca gtgggctcca cggcaatgat ttgcccggcc cgaatattcc ccacggtcac
     6061 catcttgtcg ccaatttgcg cccagaccgc agcattttcg atggtttgca tataacgggc
     6121 aggtaatgcg gcactgacgc ttgcggtaaa cagcatcagc aggagaataa cagcttgttt
     6181 gtacatgaaa aaataaccga gggtgaaaca acgcttttat tgtagcaaaa gtgccatcag
     6241 aatcgcgggt taatactcat acaaattatg tttgaggacg aaaggcggat ttttttgttg
     6301 ctggtgccaa agactactca cctccggcgc acccagttcg acaatacgct cgcgaaactc
     6361 tgcgctggat aacgtttcac ggtagaccag catctgctca attaacggta acgtgacacc
     6421 agaaatcact tcgcaacggg agtgtttgtg gcttaataat gaagccacgc ggtacggtgg
     6481 cgctccggct atatcggtta agaagatgac accctcacca gagtccgctt catgcagagc
     6541 atcgcacatc atgcggctga gcatattaga acttaatccg caccagaaat tcacggcccg
     6601 gcactgaaga agtgccccat gttttttctc cagcgcatcc agtatctctt gcgcccggtc
     6661 atcgtgacag gtaattaccc aacctaacat cgccttcctc cttagccgaa aatcagtcta
     6721 ccctgaccat tgttgataaa gggtgatagc cgtcaaaact cctctccccc aaatccagag
     6781 gagaggaaat agccttagct acgcaaacca cgtccacgtt ggatcagcga ccaacagatc
     6841 aaataaaacg ccacaataaa gaccaccagt acaccgaagg tagtgaccag cggaacatca
     6901 ttgataccga ggaagccgta gcggaaacca ctgatcatat aaacgattgg gttcaggtgc
     6961 gacagccctt gccagaacgg cggcaacaaa gtcagtgagt aaaagacccc gcccaaatac
     7021 gtaagtggcg ttaacacaaa ggttggcacc aggctgatgt catcgaacgt tttggcaaac
     7081 acgccgttca gcaaacccgc aagggagaac aacaccgccg tgagcaccag cgttaaagcg
     7141 acaaataccc acgaatgcac ctgaaacggc acaaaaaaca gtgaaattgc cgtcaccaga
     7201 atgccaacaa acagaccacg cgccacgcca ccgccgacat atccggcaat aatgacgtga
     7261 gtcggaaccg gcgctaccag cagctcttca atattacgct ggaacttggc accaaaaaat
     7321 gatgacgcga cgttagcgta ggcgttggtg atcaccgaca tcatgatcag ccccggtaca
     7381 atgaactgca tgtagctgaa gccatgcata tcgccaatac gcgaaccaat caggttaccg
     7441 aagataataa agtaaagggt catggtgatg accggtggca ccagcgtctg cacccagata
     7501 cgcataaagc gatggatctc tttcgcccag atgcttttta gtgccaccca gtaaagatgc
     7561 atcatgcgcg atctccttgt ttttcattaa ccagtgaaac aaacagctct tccagacggt
     7621 tagctttgtt acgcatactt aatacctgaa tgccctgctc acttaactgc gtaaatacgc
     7681 tgttgatccc ctgctcacgc agcacttcaa cttccagcgt cgcggtatcg accagtcgat
     7741 actgatagcc atcgagcttc ggtaacgggc ttttcggtgc gagatcgaga ataaaggttt
     7801 ccgatttcag ctttgccagc agcgccttca tcgaggtatt ttccaccagc tcaccgtgtt
     7861 gaataatgcc gatattgcgg cacagcattt ctgcttcttc caggtagtgt gtggtgagaa
     7921 tgatggtggt gcctttgtcg tttaaatcct tcaaaaagcc ccacattgag cggcgaagtt
     7981 caatatccac gcctgcggta ggctcgtcga gaatcagtag tttaggttca tgcattaacg
     8041 cacgggcaat cattaaacgg cgcttcatcc cgccagataa catacgcgca cgttcgttgc
     8101 gttttcccca tagatcgagc tgtttaagat acttttcgct gcggatgtac gcttctttgc
     8161 gctccacacc gtagtaccct gcctgattca ccacaatttg ctgcacggtt tcaaacgggt
     8221 tgaagttaaa ttcctgcggc accagtccca actgacgttt agcgttcacg acatccttct
     8281 cgagatcgta accaaatacg ctgacccgcc cggaggtttt atttactaga gagctgataa
     8341 taccgatagt ggtcgatttc ccggccccgt tcggcccgag aagcgcataa aaatcacccg
     8401 cttcgacctg caaatctatt ccacgaagcg cctgaacgcc gcctggatag gtttttttaa
     8461 gctgttgaag ttccagtgca atggtcataa atttttactt accttacgtt cttacacttt
     8521 atctgtggtt taaatcgtcc cggagttgcc ctatattagc caaacgtaat tatttggtta
     8581 caggtcgtta acctccatga aagacataga tacactcatc agcaacaatg cactatggtc
     8641 aaaaatgctg gtggaagagg atcccgggtt ttttgagaaa ctggcacaag cgcaaaaacc
     8701 gcgctttcta tggattggat gttccgacag tcgcgttcct gcagaacgtt taaccggtct
     8761 tgagccgggc gaactctttg ttcaccgtaa tgttgctaac ctggtcattc acactgacct
     8821 gaactgcctt tccgtggttc agtatgcagt ggatgtactc gaagttgaac acattattat
     8881 ctgtggccac tacggttgtg gcggcgtaca agccgcagtt gaaaacccgg aactggggct
     8941 tatcaacaac tggctgctgc atatccgtga tatctggttc aaacatagct cattgctcgg
     9001 cgaaatgccg caagagcgcc gtctggatac cttgtgtgaa ctgaacgtca tggaacaggt
     9061 gtataacctg ggccactcca ccattatgca atcagcgtgg aaacgcgggc agaaagtcac
     9121 cattcacggc tgggcctacg gcattcacga cggcttgctg cgtgatctgg atgtcacagc
     9181 gaccaaccgc gaaactcttg agcaacgtta ccgtcacggg atttccaatc tcaagctgaa
     9241 acacgccaac cacaaataaa aatgccatgc cggatgcaac acatccggca acttcacact
     9301 tactcgtcca gcagaatcac tttgccgata tacggcagat gacggtaacg ctgtgcgtaa
     9361 tcaatgccgt aacccaccac aaactcatcc gggatcgaga aaccgatgaa ttctaccggg
     9421 acgttcactt cacgacggga cggtttatcc agcagcgtac aaatcgccaa cgacttcggt
     9481 tcgcgcaggc ttaagatctc acgcactttc gacagtgtat tacccgagtc gatgatatct
     9541 tcaacaatca gcacgtcctt gccacggata tcttcatcca gatctttgag gattttcaca
     9601 tcacgggtgg tggacatgcc gctaccgtag ctggaggcgg tcataaaatc gacttcatga
     9661 gatacctgaa cttcacgaca caggtccgcc ataaacataa atgagccacg cagcagaccc
     9721 accagcacca tatcgctgcc gctgtctttg taacgctcag taatctgacg acccagttcg
     9781 gcgatacgcg ctttaatctc cgcttcgggg atcattactt ctacagtatg tttcatatct
     9841 ctaaccatat gatttaaaaa caaatcactt cgcacgggca ggttttggca ccgcaacaaa
     9901 gcgcaagaca cgcagtatac cagtaaaact attcattcgc ggaatctgtt aataaagctc
     9961 gaattgt
//
LOCUS       DACBVI010000160         9855 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.161, whole genome shotgun sequence.
ACCESSION   DACBVI010000160 DACBVI010000000
VERSION     DACBVI010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.161"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(249..2822)
                     /gene="clpB"
                     /locus_tag="IU325_19210"
     CDS             complement(249..2822)
                     /gene="clpB"
                     /locus_tag="IU325_19210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP5811134.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2952..3683)
                     /gene="pgeF"
                     /locus_tag="IU325_19215"
     CDS             complement(2952..3683)
                     /gene="pgeF"
                     /locus_tag="IU325_19215"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HAP5811135.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGRDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASTAFIQHGDKYLADISLLARQRLANVGVEQIFGGNRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3680..4660)
                     /gene="rluD"
                     /locus_tag="IU325_19220"
     CDS             complement(3680..4660)
                     /gene="rluD"
                     /locus_tag="IU325_19220"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HAP5811136.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4795..5532
                     /gene="bamD"
                     /locus_tag="IU325_19225"
     CDS             4795..5532
                     /gene="bamD"
                     /locus_tag="IU325_19225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HAP5811137.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5803..6144
                     /gene="raiA"
                     /locus_tag="IU325_19230"
     CDS             5803..6144
                     /gene="raiA"
                     /locus_tag="IU325_19230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP5811138.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6247..6372
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6248..6295
                     /locus_tag="IU325_19235"
     CDS             6248..6295
                     /locus_tag="IU325_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811139.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6394..7554
                     /gene="pheA"
                     /locus_tag="IU325_19240"
     CDS             6394..7554
                     /gene="pheA"
                     /locus_tag="IU325_19240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HAP5811140.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7597..8718)
                     /gene="tyrA"
                     /locus_tag="IU325_19245"
     CDS             complement(7597..8718)
                     /gene="tyrA"
                     /locus_tag="IU325_19245"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HAP5811141.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8729..9799)
                     /gene="aroF"
                     /locus_tag="IU325_19250"
     CDS             complement(8729..9799)
                     /gene="aroF"
                     /locus_tag="IU325_19250"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HAP5811142.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
BASE COUNT         2484 a         2626 c         2331 g         2414 t
ORIGIN      
        1 cccgctacag agtcaagcat tttttgcttt tctctgttga gattctcagg agaacctcgc
       61 tgacccggcg gcgtgcttgc cgttgttccg tgtcagtggt ggcgcattat agggagttat
      121 tccggcctga caagcgaaaa atataaaaac tttatcgttc gctcattttt caggcaaaac
      181 atcttaaata tagtcttttc cgtctaactt atagacaaaa acgagccccg aagggctcgt
      241 tttatcattt actggacggc gacaatccgg tcttcattaa cttccaggcg aatcacttta
      301 cccggaacca attcaccaga cagtatttgc tgtgccagcg ggttttcaat ctgctgctga
      361 attgcacgtt tcagaggacg tgcgccatag accggatcgt aaccgttctc gctcagcagt
      421 ttcagcgcct cgtcagaaat gtggatttca taaccacgtt cttccagacg tttatacaga
      481 cgtttcaact gaatctgcgc aatcgacgca atgtgctgtt cacccagcgg atggaagacc
      541 accacttcat cgatacggtt aatgaattcc ggacggaagt tatggctaac cacaccgagc
      601 accagctctt tcatgtgcgc ataatccagt tcaccgaagc gttcctgaat cagatcggaa
      661 ccgaggttag aggtcataat gacgaccgta ttacggaagt cgaccgttct cccctgcccg
      721 tcagtcagac gcccatcatc cagtacctgc aacagaatgt tgaagacatc cggatgcgct
      781 ttttccactt catccagcag gataacggaa tacggacgac gacgcacagc ttcggtcagg
      841 tagccacctt cttcataacc aacatatccc ggaggcgcac caaccagacg agacaccgaa
      901 tgtttctcca taaactcgga catatcgata cggaccatcg cctcgtcgct atcaaacata
      961 aagttcgcca gcgccttaca aagctcagtt ttccccacac ccgttgggcc gaggaacagg
     1021 aatgaaccaa tcgggcgatt tggatccgcc agccccgcac ggctacgacg aatagcgtta
     1081 gataccgcat caaccgcttc gttctgacca ataacgcgat ggtgcagttc ttgctccata
     1141 cgcagcagtt tttcgcgctc gctttccatc atgcgagaaa ccggaatccc cgtccaacgc
     1201 gccagcactt cagcaatttc ggcatcggtc actttattac gcaacagacg catagttttg
     1261 ccttcgagct gcgttgcggc ttccagttgc ttttccagtt ccgggatttt gccgtattgc
     1321 agttcagaca tccgcgccag gtcacccaca cggcgagcct gttcaatagc gattttcgcc
     1381 tgttccagtt ccgctttaat ggtctgcgta ccagaaagcg atgctttctc tgctttccac
     1441 tcttcttcta actcggagta ctgacgttct ttgtcgctca gttcttcgtt gagcatatcc
     1501 aggcgttttt tactggcttc atcagactct ttcattaacg cctgttgttc cagtttgagc
     1561 tggatgatac gacgatcgag tcggtcgagt tcttctggtt ttgagtcaat ctgcatacga
     1621 atgctggatg ctgcttcatc gatcaggtcg atggctttat caggcagctg acggtcagca
     1681 atgtagcgat gagacaacgt cgccgctgca acaattgccg ggtcagtaat ttgcacatgg
     1741 tggtgcaatt cgtaacgttc tttcaggcca cgcagaatcg caatggtatc ttcaacagaa
     1801 ggctcggcaa caaacacttt ctggaaacga cgttccagcg cagcatcttt ttcgatgtac
     1861 tggcgatatt cgtcaagcgt cgtggcacct acgcagtgca attcaccacg cgccagcgcc
     1921 ggtttcagca tgtttccggc gtccattgcg ccatcggctt tacccgcgcc gaccatggta
     1981 tgtaattcgt cgataaacag gatgacgttg ccttcctgtt tagcaagatc gttaagtacg
     2041 ccttttaaac gttcttcaaa ctcaccgcga tatttcgccc cagccaccag cgcgcccata
     2101 tccagcgcca gtacgcggcg gcctttcaac ccttccggca cttcgccgtt gataatacgc
     2161 tgcgccagac cttcaacgat ggcagtttta ccgacgccgg gttcaccaat cagtactggg
     2221 ttatttttag tacgacgttg cagcacctga atggtacggc gaatttcttc atcacgacca
     2281 atcaccggat cgagtttgcc ctgttcggct cgttcggtaa ggtcgatggt atattttttc
     2341 aaagcctgac gttggtcttc agcaccttga tcgttcacgc tttcacctcc acgcatttgt
     2401 tcaatcgctt gagtaatgtt ggcggtggtc gcccctgctg ctttcaggat gtcggccagc
     2461 gtgccgcgag actcaagtgc cgccagaacg aacagttctg acgagataaa gttatcacca
     2521 cgtttttgcg ccagcttgtc gcaaagatta agaacgcgca ccagatcttg tgatggctgg
     2581 acatcaccac cagtaccttc aacctgcggt aaacgattta atgcctgatt gatatctgtg
     2641 cgcaactggc cagcatttat gccagcggat gttaataaag gactaaccga acccccttcc
     2701 tgattcagca gggcgctcat taaatgaagt ggttcgatga attggttgtc gtgcccgagt
     2761 gcaagtgatt gggcatcggc aagagcaagc tggaatttat tagtaagacg atccagacgc
     2821 ataactcctc ccataacgga tcaaagttgc tactggagat taaatgaggt catccctcaa
     2881 ttattcaagg ttattgacca gattaatgtg aaaagaaaat cacgcgtacc ggatcgtctt
     2941 gattctttag gttatatcag ccaaatgaaa cttgccatac gaccggtggt cttgtcgcga
     3001 cgataagaga agaaagtctc attttccgta tatgtacaac ggttgccgcc gaaaatttgt
     3061 tcgacaccga cgttcgccag acgctgccgg gcaaggagag aaatatccgc cagatactta
     3121 tcaccatgct gaatgaaagc tgtactcgct ttggcgtcta ctgccataaa cgcctcgcga
     3181 acctccgccc ccacttcaaa cgcgcgtgga ccaattgccg gccctaacca ggcgagaata
     3241 ttttccggat tatcagcaaa acaggaaacc gtctcctcca acacgccagc gcacagtcca
     3301 cgccagccag catgagcggc ggcgacttcc gttcccgctc gattgcaaaa cagcacgggg
     3361 aggcagtcgg cagtcatcac tgcgcaaacc gtgcccggcg tgttgctata agaggcatct
     3421 gctcgttttg aggcataagg ttcgccagta agcttaagca catctctgcc gtgtacctgc
     3481 tcaagccaga ccggttttga aggcaaattg cccgcagcaa aaagtcgctt gcgattctcc
     3541 tcaacgtgat ccgggttatc gccacaatga gcaccgaggt tgagcgagtc atacgggggc
     3601 aagctcacgc cgccgatacg agtagagcta caggccgcaa caccttttgg caggggccac
     3661 tgcgggacaa tcagcttact cataaccagt ccacttcatc tttatgctct tcgaaatcgg
     3721 cgcgcatcac ctcaatcagc tccaccatat cttgtggaat aggcgcatgc cattccattt
     3781 cgatgccgga gattgggtga taaagacgca gcatggttgc atgaagcgcc tggcggtcaa
     3841 acttacgcag cgtggagata aacgcttccg aagcaccttt tggcggacgc ggacggccac
     3901 cataaaccgg atcgcccacc agcggatgag tgatatgggc catatgcacg cggatctggt
     3961 gcgtacgtcc agtttccaga cgcaaccgca gacgcgtgtg cacacggaag tgttccatga
     4021 tgcgatagtg agtgaccgcc ggtttgccca tgggatgcac cgccatgtga gtacgtttgg
     4081 tcgggtggcg gctgattggt tcatctactg taccgccagc ggtcatatga ccaattgcca
     4141 ccgcttcata ctcacgagta atttcacgcc gttgcaaaga ttcgactaaa cgcgtctgag
     4201 ccggaacggt ttttgccaca accatcaggc cggtggtgtc tttatccaga cgatggacga
     4261 tgcccgcacg cggtacatcg gcaatgggcg gatagtaatg aagtaacgca ttcagtaccg
     4321 taccatccgg gttacccgcg ccaggatgta ccaccaggtc gcgtggttta ttaatgacga
     4381 tgatatcttc atcttcatag acgatatcca gcgggatatc ctgcggttca aaacgcgctt
     4441 cttcttcaat ctcagcgttg atggccacct gctcgccacc caatactttt tctttcggct
     4501 tatcacaaac tttgccgtta accagcacgc gctggtcgag gatccattct tttatccgcg
     4561 aacgtgaata atccgggaac atttcggcca aagcctgatc taagcgttga ccgagttggt
     4621 tttcggacac cgttgcagtg agctgtactc gttgtgccat atatactgct tcttcgttta
     4681 tcgttggggt tttacggctt tgccgtttaa tatagtgtgc tattgtagct ggtcttaacc
     4741 gggagcagga acagagaatc tcccgtatta cattttgagg aaagtcaaaa cgtcatgacg
     4801 cgcatgaaat atctggtggc agccgccaca ctaagcctgt ttttggcggg ttgctcgggg
     4861 tcaaaggaag aagtacctga taatccgcca aatgaaattt acgcgactgc acaacaaaag
     4921 ctgcaggacg gtaactggag acaggcaata acgcaactgg aagcgttaga taatcgctat
     4981 ccgtttggtc cgtattcgca gcaggtgcag ctggatctca tctatgccta ctataaaaac
     5041 gccgatttgc cgttagcaca ggctgccatc gatcgtttta ttcgccttaa cccgacccat
     5101 ccgaatatcg attatgtcat gtacatgcgt ggcctgacca atatggcgct ggatgacagt
     5161 gcgctgcaag ggttctttgg cgtcgatcgt agcgatcgcg atcctcaaca tgcacgagct
     5221 gcgtttagtg acttttccaa actggtgcgc ggctatccga acagtcagta caccaccgat
     5281 gccaccaaac gtctggtatt cctgaaagat cgtctggcga aatatgaata ctccgttgcc
     5341 gagtattata cagaacgtgg tgcatgggtt gccgtcgtta accgcgtaga aggcatgttg
     5401 cgcgactacc cggataccca ggctacgcgt gatgcgctgc cgctgatgga aaatgcatac
     5461 cgtcagatgc agatgaatgc gcaagctgaa aaagtagcga aaatcatcgc tgcaaacagc
     5521 agcaatacat aacaaaaacc tgaaacacaa aacggcagcc cttgagctgc cgttttttta
     5581 ttctgttagt tgtgaaactg aagcgattta gtcactatcg atctcatcaa atatggctcg
     5641 ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc gatttctcac aaaaaagatt
     5701 cgttgacaaa aagtgacaaa attatgagat ttccatcaca cattttgaca tcaggaacgg
     5761 tatgctgaat tcaccaagac gggaagacaa gaggtaaaat ttatgacaat gaacattacc
     5821 agcaaacaaa tggaaattac tccggccatc cgccaacatg tcgcagaccg tctcgccaaa
     5881 ctggaaaaat ggcaaacaca tctgattaat ccacatatca ttctgtccaa agagccacaa
     5941 gggtttgttg ctgacgccac aatcaataca cctaacggcg ttctggttgc cagtggtaaa
     6001 catgaagata tgtacaccgc aattaacgaa ttgatcaaca agctggaacg gcagctcaat
     6061 aaactgcagc acaaaggcga agcacgtcgt gccgcaacat cggtgaaaga cgccaacttc
     6121 gtcgaagaag ttgaagaaga gtagtccttt atattgagtg tatcgccaac gcgccttcgg
     6181 gcgcgttttt tgttgacagc gtgaaaacag tacgggtact gtactgaagt cacttaagga
     6241 aacaaacatg aaacacacac cgtttttctt cgcattcttt tttaccttcc cctgaatggg
     6301 aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa taaggcctcc caaatcgggg
     6361 ggcctttttt attgataaca aaaaggcaac actatgacat cggaaaatcc attactggcg
     6421 ctgcgagaga aaatcagcgc gctggatgaa aaattattag cattactggc agagcggcgc
     6481 gaactggccg tcgaggtggg aaaagccaaa ttgctctcgc atcgcccggt acgtgatatt
     6541 gatcgtgaac gcgatttact ggaaagatta attacgctcg gtaaagcgca ccatctggac
     6601 gcccattaca ttactcgcct gttccagctc attattgaag attccgtatt aactcagcag
     6661 gcgttgctcc agcaacatct caataaaatt aatccgcact cagcacgcat cgcttttctc
     6721 ggccccaaag gttcttattc ccatcttgcg gcgcgccagt atgctgcccg tcactttgag
     6781 caattcattg aaagtggctg cgccaaattt gccgatattt ttaatcaggt ggaaaccggc
     6841 caggccgact atgccgtcgt accgattgaa aataccagct ccggtgccat aaatgacgtt
     6901 tacgatctgc tgcaacatac cagtttgtcg attgttggcg agatgacgtt aactatcgac
     6961 cattgtttgt tggtctccgg cactactgat ttatccacca tcaatacggt ctacagccat
     7021 ccgcagccat tccagcaatg cagcaaattc cttaatcgtt atccgcactg gaagattgaa
     7081 tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac aggcaaaatc accgcatgtt
     7141 gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt tgcaggtact ggagcgtatt
     7201 gaagcgaatc agcgacaaaa cttcacccga tttgtggtgt tggcacgtaa agccattaac
     7261 gtgtctgacc aggttccagc gaaaacgacg ttgttaatgg cgaccgggca acaagctggt
     7321 gcgctggttg aagcgttgct ggtactgcgc aaccacaatc tgattatgac ccgtctggag
     7381 tcacgcccga ttcacggtaa tccgtgggaa gagatgtttt atctggatat tcaggccaat
     7441 cttgaatcag cggaaatgca aaaagcattg aaagagttag gggaaatcac ccgttcaatg
     7501 aaggtattgg gctgttaccc aagtgagaac gtagtgcctg ttgatccaac ctgatgaaaa
     7561 ggtgccggat gatgtgaatc atccggcgct ggattattac tggcgattgt cattcgcctg
     7621 acgcaataac acgcggcttt cactctgaaa acgctgtgcg taatcgccga accagtgctc
     7681 caccttgcgg aaactgtcaa taaacgcctg cttatcgccc tgctccagca actcaatcgc
     7741 ctcgccgaaa cgcttatagt aacgtttgat taacgccaga ttacgctctg acgacataat
     7801 aatgtcggca taaagctgcg gatcctgagc gaacagtcgc ccgaccatcg ccagctcaag
     7861 gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc tgaacatttt cttctgccag
     7921 gtgcagcccg taagcaaaag tagcaaagtg gcgcagtgcc tgaataaacg ccatattctg
     7981 atcgtgctcg acggcgctaa tacgatgcaa ccgagcgccc cagacctgaa tttgctccag
     8041 aaaccattgg tatgcttccg gcttacgtcc atcacaccag accacaactt gctttgccag
     8101 gctaccgctg tccggaccga acattgggtg tagacccagt accgggccat cgtgcgccgc
     8161 cagcatggcc tgtaatggtc catttttcac cgatgccaga tcaaccagaa tacaatcttt
     8221 cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg tggattggca cactaacaat
     8281 caccattccg gcatcggcaa caatatcagc cgctcgatcc cagtcatgtt gctccagaat
     8341 ccgcacctga taacccgata gtgtcagcat cttctcgaac aggcgtccca tctgaccgcc
     8401 accgccgacg ataaccactg gacgcagtga cggacaaagt gttttaaatc ctttgtcgtt
     8461 ttcactggag taagattcac gcatcacccg acgcaaaaca tcctcaatca gatctggcgg
     8521 tacacccaga gcttccgcct ctgcacgacg cgaggccaac atagatgcct cgcgctccgg
     8581 aacataaata ggcagtccaa agcggctttt cacctcgccc acttcagcaa ccagttccag
     8641 acgcttcgct aataaattca gcagcgcttt atcgacttca tcaatttgat cgcgtaatgc
     8701 ggtcaactca gcaaccataa taaacctctt aagccacgcg agccgtcagc tgcccgttca
     8761 gatcctgatg aatttcacgc agcaaggcat cggtcatttc ccaactaatg caggcatcgg
     8821 ttacggatac accgtatttc atttcactgc gcggttgctc ggaagactga ttgccctcgt
     8881 ggatattact ttcgatcatc agaccaataa tcgagcgatt gccatctttg atctgagcaa
     8941 ccacggactc tgccaccgca ggctgacggc gataatcttt attggaatta ccgtggctgc
     9001 aatctaccat cagagacggg cgcagtcccg cctgttccat ctctttttca cattgcgcaa
     9061 catccgcagg gctatagttc ggcgctttac caccgcgcag gatcacatgc ccgtccggat
     9121 tcccctgagt ttgtagcaac gccacctgcc ctgcctggtt aatgccaaca aaacggtgcg
     9181 gctgggcggc ggcgcgcata gcgttaattg ctgttgccag actgccgtcg gtgccgtttt
     9241 taaaaccaac cggcatggaa agcccggagg ccatttcacg gtgagtttgc gattccgttg
     9301 tacgagcacc aattgctgac cagctaaaca gatcgcccag gtattgcggg ctatttggat
     9361 ctaacgcttc cgtcgccagt ggcagtccca tattcaccag ctcaagcagc aatttacgcg
     9421 cgatctgcag cccggcttct acatcaaaag agccatccat atggggatcg ttaattaacc
     9481 ctttccagcc gacagtggta cggggttttt caaaatagac gcgcattacc agatagaggc
     9541 tatcgctgac ctctgcggca agggctttaa atcgacgagc atattccaga gcagtttccg
     9601 gatcatgaat ggaacaagga ccacatacta ccaacagacg aggatcgcgc ccggcgataa
     9661 tatctgaaat ggttttacgc gagtcagcaa tctgggcttc ttgttgcagg ctcaatggaa
     9721 aagcggcctt cagttgttcc ggagtcatta aaacctgttc gtcggtaata tgtacgttat
     9781 tcagcgcgtc tttttgcatg atggcgatcc tgtttatgct cgtttgcgat agttgatcct
     9841 cagcgaggat gacgt
//
LOCUS       DACBVI010000161         9790 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.162, whole genome shotgun sequence.
ACCESSION   DACBVI010000161 DACBVI010000000
VERSION     DACBVI010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.162"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            1..911
                     /locus_tag="IU325_19255"
                     /pseudo
     CDS             1..911
                     /locus_tag="IU325_19255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1011..1871)
                     /locus_tag="IU325_19260"
     CDS             complement(1011..1871)
                     /locus_tag="IU325_19260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HAP5811143.1"
                     /translation="MNIAIVTINQENAATASWQAAQDFSGCTLAHWKIEPQPAVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGADKNATLPSGMQQLNIVPGALPDWLVSIENLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHAECQ"
     gene            complement(1868..2647)
                     /locus_tag="IU325_19265"
     CDS             complement(1868..2647)
                     /locus_tag="IU325_19265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HAP5811144.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTSMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQYQNPLELIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDAPFI
                     TLEQRTENGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAVGKAAIIRKTVAAESPA
                     MQCLQLARAEQQRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(2625..4034)
                     /locus_tag="IU325_19270"
     CDS             complement(2625..4034)
                     /locus_tag="IU325_19270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5811145.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGIKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISESPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVATATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLAAVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(4056..5510)
                     /locus_tag="IU325_19275"
     CDS             complement(4056..5510)
                     /locus_tag="IU325_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HAP5811146.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWTLLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(5580..6365)
                     /locus_tag="IU325_19280"
     CDS             complement(5580..6365)
                     /locus_tag="IU325_19280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP5811147.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILINNA
                     GICKLNKVLNFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            6684..7961
                     /locus_tag="IU325_19285"
     CDS             6684..7961
                     /locus_tag="IU325_19285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5811148.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPIAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGYWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            7988..9466
                     /locus_tag="IU325_19290"
     CDS             7988..9466
                     /locus_tag="IU325_19290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP5811149.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKTWAWSED
                     AAVMEKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKLLPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDAKAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFMPVESNAKRY
                     NAMNKGIFKELTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         2504 a         2488 c         2414 g         2384 t
ORIGIN      
        1 gcatatctga aagccatgca ggagcatctc ctggaaaaaa agcgccagga gctggagaaa
       61 aaacgaaagt catccggagc ctgaggtgcg gacactgcca gtctgacctg ttaaaggctg
      121 cagggctggc cagaagcacg ctgtactacc agctgtcact gcagaaggct aaggacaagt
      181 atgctgatgt gaaacagctc atagcctcca tcttccatga acaccgggga tgttatgggt
      241 accggcggat ccattgtgag cttcagaaaa gaggtcttaa gttcagtggc aaaacagtgc
      301 gaaaactgat gcaacagctc ggcctgaagt ctcctgtgcg cctgaagaaa taccggtctt
      361 accggggaaa tatgggactc gctgcagaaa atatccttca gcggcagttc aaagcggaag
      421 ctccctgtga gaaatgggtg acagatatca ccgaattcag ggctggcgga caaaaactgt
      481 acctgtcccc gatacttgat ttgtttaacg gggaaatcgt ggcgtgggaa actgcctgca
      541 ggcctacaga ggagctggtg aagcgcatgt taaataaggg gctggaaagc cttgcagaag
      601 gagaaaaacc gctgctccac agtgatcaag ggtggcatta ccggataaaa agttatcagt
      661 ctgacctggc agacaaaggg ttagtgcaga gcatgtcgcg caagggcaac tgtcttgata
      721 atgcggtaat ggagaatttc tttggtcacc tgaaagaaga aatttactat cgccgggact
      781 acagaaatgt agaagagctg gaaaatgccg ttaacgaata cataacttac tggaatcaaa
      841 aaagaataaa actcagtctt gggggactca gtccggtaga ataccggact gagcatcaaa
      901 aagccggtta actgaaactg tccaggtttt ggggttcagt tcattgacgc gtcttatcag
      961 gcttacaagt ccccagcccg aaaccgtagg caggatcatc cggctgcacg ttattggcat
     1021 tctgcgtgaa tgtttgtcac cagtgcctcc agcaccactt tccagtcatc aaccacgcca
     1081 acatctgcct gtgaaaatac cgctgcgctg gcgtcatgat taatcgccac cacaaacttg
     1141 ctattgcgta cgcccgccat taacgctgct gcgccggaag cgccaacaac aatacacacc
     1201 tcaggtgcca gcagatgtcc agaaatacca atcacttttt cggcatcaac gccaccgttc
     1261 atcactcgtg cccgactgta gcccacttcg gctcccagct tttctgccag catagcgatt
     1321 tcctggttat ctgcctctcc gccctgcccc accaccagca cccgtctggc ctcagctaat
     1381 gggtcgcggg taacattttt caggttttct atgctaacca gccagtcggg aagcgcacct
     1441 gggacaatgt tcagttgctg cataccagag ggcaacgtgg cgtttttatc tgctcccgcc
     1501 tgacgtgcca gcgaaaggca tagcgggcgt ttttctgttt gcagcgtcgc cgttagcgca
     1561 tttccccagt gggatttgcg cacgcttacg gttggaatat ctaacgaagt cacctggcag
     1621 atgcttgcac catgtaaacg ccaggccagc cgggtcgaca gttcatcgcc aaacgttccc
     1681 ggcggaaaga gaacaacgtc cgctggcgtt cgctgccact gttccaccag cgcatccagc
     1741 acctgttccg ccacagcggg ctgaggttca atcttccagt gcgccagggt acaaccgctg
     1801 aaatcctgcg ccgcctgcca gctcgctgtt gcggcattct cctgatttat tgtcacaatt
     1861 gcgatattca tggctgcatc ctttggcgta aatagtcctg ccatagcttt tgcgcttttt
     1921 ctgctaccgt ttgcccatca atcagcgtcg ctccgcgttg ttgctctgct cttgccagtt
     1981 gcagacactg catcgctggc gattcggcag caacagtttt acggataatt gctgctttcc
     2041 ccaccgccat tcgctgacgc attcccggta ctggcaaggc cacttcaccg cactgacgca
     2101 cggcaatcac cgcaggcaag cgaacccggc aacagcgcag cccgttttca gtacgctgtt
     2161 caagggtgat aaacggcgcg tcgagagtaa aacgctccac ctgggtgaag cagggccagc
     2221 ccagcatttc cgccagcaaa aatggcgttt gcccattctg cccttcgctg ctttggcagc
     2281 cagtaattat cagctccagc ggattctggt actgccattc ggcaatatgg cgagcgacaa
     2341 attccggcgc aaagcgcaga tctgccgccg tctccagcaa tacagcttcc tcaaacccaa
     2401 gagccataag atagcgcagc cagtgcaacg cccgttcatc ccccatgctc aacgcggtta
     2461 aagacatcga cgtaccgttt tttctctgcg ccaacagcag cgcggcggca gcctgttcat
     2521 cagcaccgag caaacttcgc agaagcgaaa catccggtcc gctattaccc tgagccgccg
     2581 cctgccactc tttttccgcc agcattccgg catccggttc ggctttaaac gctaacagaa
     2641 tgttcattcg cacctccaac atttcccgcc gccaccagcg ggagtgcttt ggtttctggt
     2701 gcccatagcc aggtcacgac aaaaccaacc aacaacactg ccgccaggag caataaggtg
     2761 acttgcattc cccactgcgc cagcacccac ggcagcaggc cagtacttac cgccgcgccc
     2821 aaccgactca tggcggtggc aaaaccgacg cccagcgagc gaatgtcagt aggaaaactt
     2881 tccgcaggca aaatgcccac cagattactg actgccgaaa tggtggtgct gaagagaaca
     2941 aaaagcagca gcgttaatga actgccggaa ggcaaacaag ccatcactac cagcgttgcc
     3001 gccagcagca aaaaacttcc cagcaaaaat ttgcgatgtg ccagcaggtg cgtcagaact
     3061 aatcccagca gcgcacccac aattaacaac gcattaagca tcaggctagc ggtcaacgca
     3121 tcttccagac caatagtctg ggcgatagtt ggcagccagg tataaatcac aaaccatggg
     3181 attacgaggc agacaaagaa gacgctgtta aacgctgtgc gccgccagta gcgcgaagag
     3241 aacaaggttt tgatgtgttt atgggtcgcc gttgccactt catcgcccag taaaacatgg
     3301 ggaccaaaat agcgatgcac gatagcgtga gcttctgcaa aacgcccctg gcgcagtagc
     3361 cagcgtggcg attctggcgt tccccagcgt aataacgtaa tcaacaatgc gggcagagct
     3421 gccgatgcca gcagccagcg ccaggcctcc gggctttcgc taataaagtg atgtccggca
     3481 atacttgcca gcacatagcc tacagtccac accacgctga acgcgcccag caaaataccg
     3541 cggtggcggc gcggggaaaa ttcagccagc aaggtgtgac cgactgagta atcgcctccc
     3601 agaccaatgc caatcaaaat gcgcagtcca ataagatgct ctggcgtggt ggcaaaaaat
     3661 tgtaaaaacg aagcaagcgt aatcagcaaa aagctgaagg tgaagatttt ttgtcgccca
     3721 atatggtcgg agatccagcc cagaaccagg ctgccaagaa acaaaccaag gagtgctgag
     3781 ccgccaatca ttcccgccat aaacggcgtc agttgcatgg cgggcgtaag ctgaataatg
     3841 gcgtaaccaa tgacgccgag gacataaccg tcggtcaggt gtgcgccgaa agtgagcgca
     3901 gcaatgcggc agtgaaaacg gttaagtggt aaatcatcca ttcgcaccgg tgaagtgttc
     3961 atctttatcc cttcattgtc cggtaataag aacggcttct cgcctgagaa gccgaaaagg
     4021 caacgccccg tcatcgcgga acgctgcctg acacattatt tttcaatcgg atagatagta
     4081 cccgtattca taatgccgtt aggatcgaac tgctttttca gaccttccag caacgtccac
     4141 gcgctgccgt gttccagctt gctccagtga acgcgatgtt taccaatgcc gtggtggtgc
     4201 accatcgaac caccgaggcg aatggtttct tcgcagatga ttttgttgag cggattgtgg
     4261 tacttgtcga tttcctcttc cggcttacag tcaacaacgt tgtagtcgta aacgaagtac
     4321 atgttggtgc cgttctggta gctgtgagag gagtgaccgc ccagcatggt gatgtcgtcg
     4381 gcgtgcggga actcggtacg aatacggtta ataacgcttt cgtagatttc gtgaatgcag
     4441 ctccagcagc cggacacttc ggtggtaaag cccatgttgc cggttttgag gatctgcaca
     4501 cgttcggcag ctactttatc cggtccccag ttcaggttgt taaaccaggt ttcgatcagc
     4561 ttgctgtcca ctcgctggca ttgcgggtaa cgtgccacga tttcagcaat cccttcgccc
     4621 gtcgccttcg caatgcgagg gttaccttca gccataaaga tcagcacgca ttttccgtca
     4681 gcaaaatggg tgaagtgttg ggtgccatct tcagcgtcat acaaacgagc gatcgacgga
     4741 cgataccctt ccaccatgat ttcacgcagg atgttgaagc cggttttcat gtcttccagg
     4801 atatagccgt agaagaggtt gttttccggg gtgaacttaa agattttcac agttacttca
     4861 gtgatatagc acaatgcacc ttcgttgccg atgatgatgt gacgaatgtc cgggcctgcc
     4921 gcgcggcgtg gtacgttttt aatgcgtgtt acggtgccat cagccaatac tgcttccaga
     4981 ccaacgacca tatcttcgat tgcgccgtag agtgtggaga actgcccgat actgcgggtt
     5041 gctaccaggc cgcccatctg cgccagcggc tttgactgcg gagaatgccc cgtggtgtaa
     5101 cctttttcac gcaacgcgtt ttccagcact tccagcggaa ctccgcattg cgccgtggcc
     5161 tgcatattct caatatcaat attaatgatt tgattcatgg cagagccgtc gagcaccacc
     5221 gagttttcca caacggtttc cagcccacct tcggtagcgg aagcaccggt acgcggcaca
     5281 ccgttaattt tgtgcgcatt cataaaattc agcacacggg atacttgttc tgtggaaccg
     5341 agttttacga ccgctgccgg aatcggcaaa gtataaatgc catgaatatc cggaaatttg
     5401 cgaaaacggt caatactgtt cttctttaat actgtttcat cggtaattac gcgatctgca
     5461 ccaacaattt cctttagctg gtcgacaatc gctgcgcgag ataaagacat agtaattcct
     5521 tcctgataat aaaaatggaa taatcattaa taagagaaaa cagaaaccac cgttatggat
     5581 tagcgcacta aataaccacc atcaaccact aataaatgtc cgttgacata attcgatgcc
     5641 ggacttgcga ggaatacggc tgcgcccatt aaatcctgag tatcgcccca acggtttgcc
     5701 ggaatatgat caagaacgcg ctgattggtt tctggattac tgcgtgtcgc cagcgtaata
     5761 tctgttgcat aatagccagg ggcaatacca tttacctgaa tattatattg acctagttca
     5821 tcacagtaag ctttggtgaa tccggcaaga gcatgtttag tggcagaata tgcaggtgac
     5881 cattgtccac ctaagtaaga gaataatgaa cagatattaa tgattttacc gcttttctgc
     5941 gggatcataa tttttgcagc ttcatagctt aactcgaatg cggcggtcag gttcacatca
     6001 atcatcgggt cccagtcggc acgaccgaag ttcagcacct tattcagctt acaaataccg
     6061 gcattgttaa tcagaatatc gacggtaccg aaacgttcgc aacaggcggc gattatcttc
     6121 tgcggcgcgc cttctgcggt gatatccacc tgcatgaagt ccacctcaac accctgtttt
     6181 tcaatcattt cctttgtttc gccgttatct ttaacgaagc ttggaataaa gatatttgcg
     6241 cctgctttgg ccagcgccat ggcaaatgcc tggcctaaac cgctattccc gccggtaaca
     6301 attgcggttt tacctttcag ggagaaaaaa tccattgaga acgcattgag agattcgatt
     6361 gacatagtta gctccaattt ccgtaaggca aaaaaaaaga aaggtaactt cccccgcgag
     6421 agggaagtta cctttctcat tatctctggt aactggagat aatttttatc ataagggcat
     6481 aatcgctaat gtgatcgtaa tcacagtgtg atattcgaat tgtcaaataa ttttaaaaag
     6541 cagaagcgtg attactctca cacaacacca ctgctttcca tgttatttct tattgcaatt
     6601 actagagacc tgagaaaagt aatccacctg aaagggcggc ttacttttct cttttttttg
     6661 tctattacaa atgggaatac gatatgcaac acaactcata tcgccgttgg ataaccctcg
     6721 cgataattag ttttagcggc ggcgttagtt tcgacctggc ttatttacgt tatatttatc
     6781 aaattcccat agcgaaattt atgggattca gcaataccga gataggttta ataatgagta
     6841 catttggtat tgcggccatt attctttatg cccccagcgg cgttattgcc gataaatttt
     6901 cacaccgcaa aatgattact tccgcgatga tcattaccgg attactgggt ctgttaatgg
     6961 caacatatcc accgctgtgg gtaatgctct gtattcaggt cgcctttgcg ataaccacta
     7021 ttttaatgct gtggtcggtg tcgattaaag ccgcctcatt gctgggcgat catagcgagc
     7081 aagggaaaat tatgggctgg atggaagggc tgcgcggcgt cggtgtaatg tcgctggcgg
     7141 tgtttaccat gtgggtcttt tctcgcttcg ctccggatga cagcgccagt ctgaaaacgg
     7201 tcatcatcat ctatagtgtg gtttacatct tgttggggat tctgtgctgg ttttttgtta
     7261 gcgataacaa caatctgcgc agtgccaata acgaagaaaa acagtcattc cagcttagcg
     7321 acatcctggc cgttttgcgc atcagcacca cctggtattg cagcatggtg atttttggcg
     7381 tcttcaccat ctacgccatt ctgagttact ccaccaacta tctgaccgaa atgtacggca
     7441 tgtcgctggt ggcggcgagc tacatgggga ttgtgatcaa caaaatcttc cgcgcgctgt
     7501 gcggcccact tggcggcatt atcaccacct acagcaaagt gaaatcccct acccgcgtga
     7561 tccaaatcct ttccgtactc ggcttgctgg cgttaactgc cctgctcgtc acgaactcta
     7621 acccgcaatc ggtcgcgatg gggattggcc tgattttact gctgggattc acctgttacg
     7681 cctcacgcgg gctgtactgg gcctgccctg gcgaagcgag aacaccgtct tacattatgg
     7741 gcaccacggt aggtatttgt tcggtgattg gattcctgcc ggatgtcttc gtttacccga
     7801 ttatcggcta ctggcaagac accctgcccg ctgcagaagc ctaccgcaat atgtggctga
     7861 tgggcatggc ggcgcttgcc atggtgatcg tctttacctt tttgctgttc caaaaaattc
     7921 gtactgctga tagcgcccct gctatggcta gcagcaagta agtccagaaa attacaggag
     7981 aaaaatcatg tcgaaaaaat acatcatagg gattgatggt ggaagtcaga gcacaaaagt
     8041 ggtgatgtac gatctggaag gtaatgtggt ttgcgaaggc aaaggcttat tgcagccgat
     8101 gcacacgcca gatgccgata ccgcagaaca tcctgacgac gatttatggg catcattatg
     8161 ctttgccggt cacgatttga tgagtcagtt tgccggaaat aaagaagata ttgtcggtat
     8221 tggtctggga tccatccgtt gctgccgtgc gttattgaaa gccgatggca cgccagctgc
     8281 gccgttgatt agctggcagg atgcacgcgt tacacgccct tacgaacata cgaatcctga
     8341 cgtggcatat gtcacctctt tttcgggtta tctgacgcat cgcttaaccg gcgagtttaa
     8401 agacaatatc gccaactatt ttggtcagtg gccggtggat tataagacct gggcatggag
     8461 tgaagatgcc gcagtcatgg agaagtttaa tatcccccgt catatgctgt ttgatgtgca
     8521 aatgccgggc actgttcttg ggcatatcac tcctcaagcc gcgctggcga cacatttccc
     8581 ggctgggctg ccggttgttt gtaccaccag tgataaaccg gtagaagcac tgggagccgg
     8641 gttactggat gatgaaactg cggtaatttc tcttggcact tacatcgcgt tgatgatgaa
     8701 cggcaaatta ctaccgaaag atccggtagc gtactggccg attatgtctt ctattccgca
     8761 aaccttgctg tatgaaggtt acggtattcg caaaggcatg tggacggtga gctggctacg
     8821 cgacatgtta ggcgagtcgt taattcagga tgccaaagca caggatcttt caccggaaga
     8881 tttgctcaac aaaaaagcgt cttgtgtgcc gccaggctgt aatggtctga tgacggtgct
     8941 ggactggctg accaatccgt gggaaccgta caaacgcggg attatgattg gctttgattc
     9001 cagcatggat tacgcatgga tatatcgttc gatactggaa agcgtggcgc tgacgctgaa
     9061 gaacaattac gacaatatgt gtaatgaaat gaatcacttt gcaaagcatg tgatcattac
     9121 tggcggcggt tcgaacagcg atctgtttat gcagattttt gccgacgtgt tcaaccttcc
     9181 ggcacgccgt aacgccatta atggttgtgc aagcctgggg gcagcgatta atacggcggt
     9241 aggtctgggg ctatacccgg attacgcaac ggctgtcgac aaaatggttc gcgtgaaaga
     9301 tatctttatg cctgttgaga gcaacgccaa acgctacaac gcgatgaata aaggcatttt
     9361 caaggagtta accaaacata ctgatgtaat cctgaaaaaa tcgtatgaag tgatgcatgg
     9421 ggaattgggg aatgcggatt cgatccagag ctggtcgaat gcgtaagtga ggaagaccgg
     9481 gcgggaaact gcccggcctg aacatacctg aatggttatc cccgctggcg cggggaacat
     9541 aagttcgaca acttaaaaaa tgataacaag tccggtttat ccccgctgat gcagggaaca
     9601 taataaaaac tttaactagt tcggtttatc cctgttttta caaggaatat taactacact
     9661 cgatgtcttt agaataaaag acaatacgaa tcgcacttta tcaattttca cttaaagtat
     9721 gaactggata tctttattca taagaactat tcatcaacag cgtatagagg cgattatgta
     9781 aaaccactca
//
LOCUS       DACBVI010000162         9712 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.163, whole genome shotgun sequence.
ACCESSION   DACBVI010000162 DACBVI010000000
VERSION     DACBVI010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.163"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..613
                     /locus_tag="IU325_19295"
     CDS             <1..613
                     /locus_tag="IU325_19295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HAP5811150.1"
                     /translation="GTILTENPKEYVQKQIKESHKESATNVTFDYKELAPIFEGVQKK
                     QIEALSNQLEHIKSFKTDYDSRLNKFARDFQYLSSAFSINRQDLLKNYQEIRASITDY
                     DDLLREIKELTISRDKSVDDKRTLFDKKRDNWNSQEVQGEVKALNEDIADSDNKIRSK
                     LTIVQNHRRENQYKANKNISNAMLNILDWFERYPDIVKNITQA"
     gene            complement(750..1415)
                     /gene="yieH"
                     /locus_tag="IU325_19300"
     CDS             complement(750..1415)
                     /gene="yieH"
                     /locus_tag="IU325_19300"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HAP5811151.1"
                     /translation="MTQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDQEEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELADIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPELMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTELGQLPALWKARSWN
                     ITR"
     gene            1582..2919
                     /locus_tag="IU325_19305"
     CDS             1582..2919
                     /locus_tag="IU325_19305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="involved in the transport or adenine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP5811152.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTECVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            complement(2973..3539)
                     /gene="yieF"
                     /locus_tag="IU325_19310"
     CDS             complement(2973..3539)
                     /gene="yieF"
                     /locus_tag="IU325_19310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent chromate oxidoreductase YieF"
                     /protein_id="HAP5811153.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKS"
     gene            complement(3561..4310)
                     /locus_tag="IU325_19315"
     CDS             complement(3561..4310)
                     /locus_tag="IU325_19315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811154.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            complement(4467..5426)
                     /gene="yidZ"
                     /locus_tag="IU325_19320"
     CDS             complement(4467..5426)
                     /gene="yidZ"
                     /locus_tag="IU325_19320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HAP5811155.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKHLYASMA"
     gene            complement(5401..6576)
                     /gene="mdtL"
                     /locus_tag="IU325_19325"
     CDS             complement(5401..6576)
                     /gene="mdtL"
                     /locus_tag="IU325_19325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HAP5811156.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMATMGIAVLMLSLFILKETRPAAPTNSDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            complement(6709..7956)
                     /gene="tnaB"
                     /locus_tag="IU325_19330"
     CDS             complement(6709..7956)
                     /gene="tnaB"
                     /locus_tag="IU325_19330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HAP5811157.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDSTSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            complement(8048..9463)
                     /gene="tnaA"
                     /locus_tag="IU325_19335"
     CDS             complement(8048..9463)
                     /gene="tnaA"
                     /locus_tag="IU325_19335"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HAP5811158.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
BASE COUNT         2548 a         2374 c         2400 g         2390 t
ORIGIN      
        1 tggaacaata cttactgaaa atccaaaaga atatgtccaa aagcaaataa aggagtctca
       61 taaagaatct gctacaaatg taacatttga ttataaggaa cttgcaccca tttttgaagg
      121 tgttcaaaaa aaacaaattg aagcgctatc aaaccaactg gaacatataa aatcttttaa
      181 gacagattac gattcaaggc tcaataagtt tgctcgcgat tttcaatatt tatccagtgc
      241 attttcaatt aacaggcagg atttattaaa aaactatcag gaaataagag cttccattac
      301 agattacgat gacttattgc gagaaattaa agagttgacc ataagtcggg ataaatcagt
      361 agatgataaa aggactttat ttgataaaaa aagggacaac tggaatagtc aagaagtaca
      421 gggtgaagta aaggcattaa atgaggatat tgctgactct gacaataaga tacgttctaa
      481 gcttactatt gttcaaaatc atagacgcga gaatcaatat aaagcgaata aaaatatcag
      541 taacgcgatg cttaatattc tggattggtt cgaacggtat cccgatattg taaaaaatat
      601 aacccaggct taactatgct gagggtggat gacaggacga ataacgacat aaattattca
      661 tagggaatag acgcttactc atcagacacc tggtattctt tatctcaaac cagcgtgact
      721 gatgtgtaag cgttgaagta aggaggcgat tatcgcgtta tattccagct gcgtgccttc
      781 cagagcgcag gcagctgccc cagctcggta aaggtggtga ctttcggatg aacgatcggc
      841 ttattgtgcg ggtcagcgca gaagtaaaac acttccatac cggcatcgat tcctgattgc
      901 gcaccggcgc tggagtcatc gaccagaatg cagttttcaa cgttcacatt catcgctttt
      961 gccgcatgga acattaactc cgggtcgggc ttccaacgct gaatatcgta gccgctgaac
     1021 agtttttccg ggaagtagtg cagcatattg agcttaccaa gcgaatgctg cattttgctg
     1081 accggaccgt tagagacgac gcacatcggc acgttaatgc tcgccagcaa ttcgttcgct
     1141 cctgcgatgt ccgcaagttc ggtatcaaac agacgtgcta cttctgcgcg gtaaacgtgc
     1201 tcggcatcgg cctggaccat cgtcacgccc tgttctttac tgatgatgtc gatgatttcg
     1261 tagagtttta cgcctttaaa acgggtaaaa atctcttcct ggtcaacgtg aatgccaaat
     1321 tccctgaaca tcgtgacata ggcgcgagag caaatgacct cgctatcgac cagcgtaccg
     1381 tcgcagtcga aaaataccgc ttctatctgg gtcatgtctt tcctttatcg ttagtttaac
     1441 gttttcgtca tgccaattcg catcacgcaa tcgttgccgt ataagcaaat acaatgaaaa
     1501 aaagttatct tcagccgtcc atattgtctc attttggtat acgatagcga cggattttcc
     1561 ctccttgttc ggaaatagat aatgagtcaa caacacacaa cccaggcttc tggccagggg
     1621 atgctggaac gcgtgtttaa actgcgcgaa catggcacga cggcacggac cgaagtgatc
     1681 gccggtttta ccaccttcct gacgatggtt tacatcgttt ttgttaaccc gcaaattctt
     1741 ggcgttgctg gcatggatac cagcgccgtc ttcgttacta cctgtctgat tgctgcattc
     1801 ggcagtatta tgatgggact gtttgctaac ctgccagttg cgctggcacc tgctatgggc
     1861 ctgaatgcgt tcttcgcttt tgtggttgta caggcgatgg gcttgccgtg gcaagtcggg
     1921 atgggcgcaa tcttctgggg cgcgataggt ctgctgttac tgacgatttt ccgcgttcgc
     1981 tactggatga tagccaacat tccggtgagt ctgcgtgtgg gcattaccag cggtatcggc
     2041 ctgtttattg gcatgatggg actgaagaat gcaggtgtga ttgtcgctaa cccggaaacg
     2101 ctggtgagca tcggtaatct gacttctcac agcgtactgc tggggatcct cggcttcttc
     2161 atcattgcta ttctggcctc acgcaacatt cacgcggcgg tgctggtttc catcgtggtg
     2221 acgacgctgc tgggctggat gctgggtgat gtccactaca atggcatcgt ttctgcgccg
     2281 ccgagcgtaa tgactgtcgt gggtcatgta gatttagccg ggtcgtttaa ccttgggctg
     2341 gcaggggtga ttttctcttt catgctggtc aacttgtttg actcctccgg tacgctgatt
     2401 ggcgtgacag ataaagcagg actggcggat gaaaaaggta aattcccgcg catgaagcag
     2461 gcactgtatg tcgacagcat ctcttccgtg accggttcgt ttatcgggac ttcttctgtt
     2521 acggcgtata tcgagtcttc ttccggcgtt tccgttgggg ggcgtaccgg cctgacggca
     2581 gtcgttgttg gtctgctgtt cctgctggtt atcttcctgt cgccgctggc ggggatggtg
     2641 ccgggatatg ccgcagctgg cgcgctgatc tacgtgggcg tactgatgac ctcaagtctt
     2701 gctcgcgtga actggcagga tcttactgaa tgtgttccgg cgtttattac cgctgtgatg
     2761 atgccgttca gcttctcgat taccgaaggt attgcgctgg gctttatctc ctactgcgtg
     2821 atgaagattg gtaccggacg tctgcgtgat ctaagcccgt gcgtaatcat cgttgcgctg
     2881 ctgtttatcc tgaagattgt gtttatcgac gctcactaaa atcgatagcg cgttaaaaac
     2941 ccgccatgca tgtttgctgg cgggtttttt atttagctct taactcgctg aataaactca
     3001 ccaaatgcag tcaattgccc ggtcaggtgg tccagcgtac cctgatcaat cacttctccg
     3061 gtttgcggat caactttgtt ctgaatcacg ccgcccataa attccggctt gttcatcacc
     3121 attgcatcga ggaaaaccag aatctggcgc aggtgatact gacagcgcgc gccgccaatc
     3181 acgcccattg agctggtctg aatcaacacc ggtttaccgg ccagaggttg atccggcagg
     3241 cgggaaagcc agtcgatggc atttttcagc ccacccggta ccgagtagtt atattccggc
     3301 gtgacgatca ccacaccgtc agcctgacgg atctgttccg ccagagcttc aaccgttgct
     3361 ggaaaacctt cttcctgctg tacgtcagcg tcatacaagg gaatgtcggc aatggaaggt
     3421 aacgcattga cttccatgct cgccggagca attttcggca gggtacgtgc aaccatgcca
     3481 ttaaatgagc ctttgcgcag gctccccagt aacgtaacca cctgcaattt ttcagacatg
     3541 atgactcctg tggttgtcta tcaatttggc gaaaaagcct tcacggtaga gagctgggca
     3601 aaacgcatca tccggctggt aggattgttg gcgcggctgt gaatatcggg caggcttttc
     3661 cagccgtgtt tgccatctaa ctcccagaca ctgagctttt caatcgtggg cgtgaccgcc
     3721 acggaccaca ccagcacgtc ctccgcgtcg cataacgctt ctacatgatt tacatgagca
     3781 catttcaggc gtccggcaat gggcagcagc tgcagatcgc gcggatcgtc attcaaaaca
     3841 tcaccggtta gttttagcac gcttcggcgc agcgtgatga gctgcgcccg cgcttcgttt
     3901 ggatcatttt gtttactgat ccatagcgat tcattgctgg aaaaggtgtg gttatcgggc
     3961 gcgtgtggac tatgaaaccc gcgcgtcgca cgctgcagtt ccatatcgag gccacattca
     4021 ccttcggttg ttaacgccac gccaaccata ttcccggcat aggaaataga aaacgaaggc
     4081 aaatttttat cgctgaaaac cggtttacct ttcggcaggg tgacgatttc cggcaattcg
     4141 ccaatgccat acagcatgaa catcagttct gcgagtaaac ctctggacgc cagaaaacgg
     4201 ctttgacgat gagggggaat gtttcgggct tcttgatgac actgggaggg gagacgaaca
     4261 gaaatcagat gtccttccgt taaaatccct cgggcaaagt gagtcgccat ttttcgctcc
     4321 atgcttaatg gtccattatg aatacaagag taatcattta ctggaatgat ggacagacta
     4381 aagcggctaa aagttcgatt cagacaagct tattacaact tttttacatt ttattgtgaa
     4441 attgggtcgc gtttcatacg attcggttat gccatcgacg cgtaaagatg tttaatggtt
     4501 tcccgtaacc agacgatctt cggattatgg ctgttccgtt tatgccacag cagggtaaaa
     4561 gggacttcca gctttttttg ctggctttcg tcaaacggga gaggaagagc aaccaacggc
     4621 agttggtgga gttgattgta gtactgacag tagcgcggtg cggtcgccag tagcagattg
     4681 tcgggctgtg ctgccataaa cagtgactgc tcgaattccg gcaggctcat agcaatcgtg
     4741 cgttcgcgcc ccagctcctg taacacattg tccagcgccc aggtatcgct ctgttcccag
     4801 caaatgctga tatgcggata acgtaaaaag gtgtccagat tccacgtttc atgcagtgcc
     4861 ggatgatctt ttcgtaacca gacgcagggc acatcactaa acagcacttc ataatcaatg
     4921 gctaacggta gcgagcttaa tagctcccgc gaacggggat gactttcgcg accggaaaaa
     4981 ccaatatcca cttcaccacg agtaatggca tctaaggaat cgtaatccca gttacgtaat
     5041 ttgatggtcg cctgcgggta acgttggtag atccgtttcg acagcgcatt aagcatgatc
     5101 atcatcagcg gcgattccgc cgccagctca aacttcaggc cgcgcggtgt ctggtgatgc
     5161 ggtttatcca gcagcaggtt gctcatttgc atccactccg ccagattttg ctccatgctg
     5221 accattagcg gtgtgggcga cagccccagc ggtgagttca caaagagcgg gtcgtcaaac
     5281 cacgctctta actttgccag cgacttactc accgccgaag gggtcacgtt catccgcttc
     5341 gccgctttgg tcacgctgcg ctcctgcatc agcagttgca gacagagcag cagattgaga
     5401 tcaagcgtgg tgatggattt cttcatgagc ggcaaccggg cgtccaggcg cgacgaacat
     5461 aatcagcaac aggctcacta tgctacaggc aatcagaatc ccgatcagca tattccatgc
     5521 gccgatacca accaccgctg ccagccaaat ccacagtgac gaaccgcaaa cctgcgcaat
     5581 acctaaggtc gagctggcta cgcccgcgcg taatgaaaac ggccctaacg cctgactcat
     5641 cgctacgcca aaacctaccg agaaaccggc gcaaatcagc gtgataccaa acagagaaac
     5701 cgcatgggaa ggtgaaacgg caagggtgat gcccgccgcc aggaataata cctgcgaggt
     5761 gatcatcaac gtacgtggct taaaaattcc cagcgcaaat ggcgtggaga atgaaacggt
     5821 catgctgacg ccagcggtca acgccataat ggtggcgtat tctccgcgct caaaacccat
     5881 gatttccatc agcaataccg gcgacgtgtt gacgaaggtg aggatcaccg aaacgctgag
     5941 ggtggtgata acaacacggc tgaggaaaaa ccggttaagc agcgactcgc tattttctcg
     6001 tggtttgtcc gaatttgtgg gggccgcagg gcgcgtttct tttaaaataa acaaagacaa
     6061 catcagtacc gcgatgccca tcgttgccat cgtccagaac agactctgcc acgggaattt
     6121 aagcataatc aggtgtccga gcactggcgc taacaccgga atgatgcagg taatgccgtt
     6181 gagtaacgac agcactttag cccgacgtcg atcatccagt gtgtcgcgta aaatggcgaa
     6241 cgcaaccacg taacaacagc ccgcgcccag cccctgtaga aagcgccctg caagaaataa
     6301 cgtgctggtt tcagccagtg aacagaacac cgaggcgata ataaatagcg ccgcgccggg
     6361 tatggcgaca ggctttctcc ctgaacgatc ggccacttta ccggcaaata acatcgcagc
     6421 tgccatgccc gccagataaa cggagaacgc aatatgcaac tgcgcttcgc tggcattgag
     6481 atcggcggcg atgcgcggta aaccaacgag gtacatatca atcccggcgg gataaagtaa
     6541 aaccagggca aaactacaaa tcaaaaagcg ggacatgggc taaagcgtcc tgatagtaag
     6601 gtgacgcaaa gcatacgtgg tgaaggagaa tcaggcgagt tgccatttgg acataagtga
     6661 tttcctgaca ggcaataagc cagcgaaatg gctggcttct tgaagggatc agccaaattt
     6721 aggtaacacg ttaaagacgt taccgaacca gcataaaatt acggttatac caaagagaat
     6781 gacaatcgcc ggaataagat tgccgcccca gaccgtgaac atctgattgg gaaacttctt
     6841 gcgagctttg attgccagca ccgcggggat aatgaccgcc cagatggtgg cacacagtcc
     6901 ggcaccgccg atcccgtaaa tgaagccgtt cgggaagatc agatacaaca acgcaggtgg
     6961 caggaaggtt aacagcacgg ttttgaaacg cccgccgtgg gagttatcaa tcttaaacag
     7021 atccgccaga taatcgaaca accccagcgt gacaccaaag aacgaactgg caacagctaa
     7081 gttggagaac accagcaggc aaaactcgat aatgccgtgc tgtttggtgc cgaggaacga
     7141 tttgaccagc gaatcaacgt tgccgcctga ggagattatc gccttaaagc tttctcgtgg
     7201 gatattcccc atcgtgcaat agagccagaa gagataaatc accagcgcca gcagcgaacc
     7261 gaagaccacg cttttgatta gcttatcttt gcgtttgcca tagcaaataa tcaggctggg
     7321 aatattgccg tggaaaccaa atgacgccag acacaccggc aaagccataa agatatacgg
     7381 gaaataagat gtacccgcag tggtgctggt ggaatcgcgc agaatggagt aatcgacctg
     7441 gaagaagaaa gaaccaaaaa caattacaaa ggagataatc ttcagcccaa ggaacaatga
     7501 ggtaatacga ctggcggcta acgagcttat ccacaatacg ctggcaacga aaatggctgt
     7561 gcagatcccg acaatacgtg gattagcgtg ataacccaaa ttcattgata tcgtttcact
     7621 aatgatcgca ccattagcag agatataggc ataagtgagg atatagagaa cgaaggcaac
     7681 ggtaataccg ctgataatgt tccaggtgtt accgattaaa tctttggtga tggtgttaaa
     7741 actggagccg acgggataat ttaaatttgc ttctaataac aacaacccgg aatgaagcat
     7801 tgaaaaccag gcaatgataa ggataaaggc accccagaaa aaccaggcac cggcaagatc
     7861 aacaggtaaa gcaaacatac ctccaccaat tactgtacct gctataacca taacacccca
     7921 aaatgcagag tgcttttttt cagcttgatc agtcatgatg ccacctttag aggaaggcta
     7981 tttttgttat tgaggatgta gggtaagaga gtggctaaca tccttatagc cactctgaat
     8041 gttttaatta aacttctttc agttttgcgg tgaagtgacg caatactttc ggttcgtagg
     8101 taaaggttaa ccctttaata ttcgccgcgt tctctttcac atgtttaaag gcttcaataa
     8161 tgaagtccat atgtgtttga gtatatgttg cacgcggaat ggttaaacgc agcagttcag
     8221 ccgggcatgg cagttgttta ccggttttcg gatcgcggcc taacaggaaa gaaccaattt
     8281 ctaccgcacg gataccggcg actttataca gctcgcacgc cagcgcctgt gccgggaact
     8341 ggtctgccgg gatatgcggc agcagtttac cggcatcaac gaatgccgcg tgaccgcccg
     8401 cctgctggca gacaacgcca atctcttcca gaccatcgac cagatactgt acctgcgcga
     8461 tacgataagc cagccagtcc agattcatgc cgtcatacag acctaccgcc agacgctcca
     8521 tcgcgccgcc ttccaggccg ccatatgtcg ggaagccttc ctgtaccacg caaagggttc
     8581 tgcattcggt gtacacatca aagaagctgt cgtctttcat gcacagcaag ccgcccatcg
     8641 gcaccatcgc atctttcttg gcggacatcg ccagcatatc ggcatatttg taggtttcgc
     8701 gggtgatctg ctcgatggtc cagtctttgt attctgcttc acgctgcttg atgaaatagg
     8761 cgttttcagc aaagcgtgcg gagtccatta ccaccggaat atcgtatttc ttcgcgatgc
     8821 tgtacatcgc ttttaagttt gccagtgaaa ccggctgacc acctgcagag ttactggtga
     8881 tggtcgcaac gatatacggc acgttattcg ggccaacttc ttcaatgccg cgttctaatc
     8941 cctcaaggtc aaagttgcct ttaaagtcgt aacgcacgcc cgtatcgaag gcttctttga
     9001 tatagacgtt acgcacggta cagccgttga tctggctatg gccctgcgtg gtatcaaaga
     9061 aatagttaga gaacgccacc attttgctgc gatccaggcc tttttcctgc tcgcgttttt
     9121 taatcagtac cggaatatag atttgttctg caccacggcc ctggtgagtc ggaatagtgt
     9181 attgataacc aaagatattt tttactgact cggctaacgc atagtagctg cggctgccgc
     9241 tgtaagcttc gtcgccgcgc atcatcgcgg cctgcatact ctgcgtcacc gccccggtgc
     9301 cgctgtcggt cagtaaatcg ataaacacat cttcgctatc cagcaggaac gggttcatac
     9361 cggatttaat aattgcttct tcacgataag cgcgagtggt acgttttact ggctcaataa
     9421 cacggatgcg gaacggttca gggagatgtt taaagttttc cattacataa tccttcattt
     9481 attttaatta cagtgatccc tgtgaatatt acatctgcaa tatccattaa ttcgccagat
     9541 atatatgcaa gcaccatcat ttactaaaac aagtaaataa acgagagatg gcctttgcaa
     9601 aaggcaaaat taagatatag caaacaaata gatcacattg aatctaatcg gtttggctct
     9661 ggtgatggct acagaagggc aaatcaaggg cgatgatcaa caatcttgtt gt
//
LOCUS       DACBVI010000163         9678 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.164, whole genome shotgun sequence.
ACCESSION   DACBVI010000163 DACBVI010000000
VERSION     DACBVI010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.164"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            200..1513
                     /gene="gltP"
                     /locus_tag="IU325_19340"
     CDS             200..1513
                     /gene="gltP"
                     /locus_tag="IU325_19340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HAP5811159.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(1592..2281)
                     /gene="yjcO"
                     /locus_tag="IU325_19345"
     CDS             complement(1592..2281)
                     /gene="yjcO"
                     /locus_tag="IU325_19345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HAP5811160.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(2375..4522)
                     /locus_tag="IU325_19350"
     CDS             complement(2375..4522)
                     /locus_tag="IU325_19350"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(4103..4105),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HAP5811161.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(4720..6186)
                     /gene="mdtP"
                     /locus_tag="IU325_19355"
     CDS             complement(4720..6186)
                     /gene="mdtP"
                     /locus_tag="IU325_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HAP5811162.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPQVQTGIPATLSYELLARRPDLQAMHWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQASPVVEKK"
     gene            complement(6183..8234)
                     /gene="mdtO"
                     /locus_tag="IU325_19360"
     CDS             complement(6183..8234)
                     /gene="mdtO"
                     /locus_tag="IU325_19360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HAP5811163.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTNSLTPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAIAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     TKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMALRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(8234..9265)
                     /gene="mdtN"
                     /locus_tag="IU325_19365"
     CDS             complement(8234..9265)
                     /gene="mdtN"
                     /locus_tag="IU325_19365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HAP5811164.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQASDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAGLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGT
                     SAVANLEPQ"
     gene            complement(9284..9559)
                     /locus_tag="IU325_19370"
     CDS             complement(9284..9559)
                     /locus_tag="IU325_19370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811165.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRIIQRANINLAFVGIIYTALFALYAMLLWLAFF"
BASE COUNT         2185 a         2591 c         2575 g         2327 t
ORIGIN      
        1 ccataaactc gcgttattat gcattaatgc agcgaaaagc tctgttgtta aagggttgcg
       61 caacataccg cgcaaatgat actgatcata agcgttaaaa aaatctacaa accaacgcaa
      121 cacaattcat gccctggcag tatgtcacgt tctcacgttt ctgaacgggg aacggcgctc
      181 cattgaggaa gttattctga tgaaaaatat aaaattcagc ctggcctggc agattctgtt
      241 tgctatggtg ctgggcattc tcctgggaag ctacctgcac taccatagcg acagccgcga
      301 ctggctggtc gtcaatttgc tctctccggc gggtgatatc ttcatccatc tgatcaaaat
      361 gattgttgtg ccgattgtga tctccacgct ggtggtgggt atcgcgggtg ttggtgatgc
      421 caaacagctc gggcgtattg gcgcgaaaac cattatttac ttcgaggtga tcaccaccgt
      481 cgccatcatt ttggggatca ctctggcgaa cgtcttccag cccggtgccg gggtggatat
      541 gtcgcagctg gcgaccgtcg atatctcgaa atatcagagc actacggaag cggtacaaag
      601 cagttcccac ggcattatgg gcacgatttt gtcgctggtg ccgacgaaca ttgtggcgtc
      661 gatggcgaaa ggcgaaatgc tgccgatcat cttcttctcg gtgctgtttg gtctggggct
      721 ttcctccctg cccgcgacgc atcgtgaacc gctggtgacc gtgttccgct ccatctctga
      781 aaccatgttt aaagtgactc acatggtgat gcgttatgcg ccggtgggtg tgtttgcgct
      841 gattgcggtg acggtggcta actttggttt ctcgtctctg tggccactgg cgaaactggt
      901 gctgctggtg catttcgcta ttctgttctt cgcgctggtc gtgctgggaa ttgtggcgcg
      961 cctgtgcggg ttaagcgtct ggatcctgat tcgtattctg aaagatgagc tgattctggc
     1021 gtactccact gccagttctg aaagcgtgct gccgcgaatt attgagaaga tggaagccta
     1081 cggtgcaccg gcgtcgatca ccagtttcgt ggtgccgacc ggttactctt ttaaccttga
     1141 tggttcgacg ctgtatcaaa gtattgccgc tatcttcatt gcgcagttgt atggcattga
     1201 cctgtccatc tggcaggaaa tcattctggt gctgacgctg atggtgacct cgaaagggat
     1261 tgctggcgtg ccgggcgtgt cgtttgtggt gctgctggca acgctgggta gcgtaggtat
     1321 tccgctggaa ggtctggcat ttattgccgg tgttgaccgt atcctcgata tggcgcgtac
     1381 tgcgctgaac gtggtgggta atgcgctggc ggtgctggtg attgccaagt gggaacacaa
     1441 atttgaccgt aagaaagcgc tggcttatga gcgtgaagtg ctgggcaaat ttgataaaac
     1501 tgcggatcaa taattgaaga ttgccgggga tatccacccg gcaatgtgtg gatgcctgat
     1561 gcgacgctta acgcgtctta tcaggcctac cttaaccgtt agttaatttt tcaaactctt
     1621 cacacccggt atcaaaccct tccatacagc tcagatttaa ccagtgcagc gccttttgct
     1681 tgttcttctc gataaaacct tcttcaccat ttaagaacat cattcccgcc cagtactcgg
     1741 aataaccggt tcgggaaatc gcagagctgc gtttgaaata ccaggttgcc ttgtcatcgt
     1801 cggccttaat gcccacgccg ttggcgtaaa tcaaaccaag cagcatttgg gcatcgacgg
     1861 cagagtcgtt ctccagatct tccgaagcgt tttctaacag cgaaatcgcc tttggataat
     1921 ccggtttacc cgcttgcgta tttaccagaa tatgcgccag cgttacttca ccttctttac
     1981 tccccgcttg cgccgctttc tctgcaagaa cttttgcttg tggatagtcc agactgactg
     2041 gattggtgat tttgatctgc gccaacagcg cgcaggcatc agcatcaccg ctgtcggcgg
     2101 ctttctgtgc ccaatattcg gctttgctca aatcgccgga gctaaaccag ctgtcggcaa
     2161 gaaaatattg tgcgcgtcgg tccccggcct ctgctgcctt taaatactgg ctgccaggct
     2221 cggagtcgtt cgcgtgggca aagaatgtca aaaacaacat caatgcgata atttttttca
     2281 ttttgataat acgattgggt agggagtaac cagtataaag agttcgaatg gataaaaaaa
     2341 cagcctccga aaggaggctg tagaaaggac tgtattacgc cagtgccgct tcgcgcaggc
     2401 gagttttcaa cttgttgtac tcgtcaatca cgtactgctc ggcggcgcgc tgatcggcga
     2461 tcggctcgac gcgcacggcg cagtatttgt attccggcgt tttcgtaatc gggcttaagt
     2521 tttcggtaac cagctcgtta caggcaccaa tccaccactg gtaggtcatg taaatcgccc
     2581 ctttgttcgg acgatcgctg acctgcgcac gggtgataat tttgccttta cgcgagtgca
     2641 cccaaaccaa tgcctcatct tcaataccca gacgtttggc gtcttcggta ttgatttgtg
     2701 cgtagccagg ttcatcagcc agcgccgcca gtgccgcaca gttaccggtc atcgaacggc
     2761 aagagtagtg accaacttca cgcaccgttg acagtaccat cggatattcg tcggtgagtt
     2821 tgtcgattgg cgctacccag tcgcaggtga agaactgcgc cagaccgttc ggggtatcaa
     2881 acttctcttt aaacaaataa gaagtcccct gatcggcatc tgaagtatcg cgacaaggcc
     2941 actgaatgaa gcccagttcg cccattttct cgtaagtcgc accgtagaaa tccgggcaca
     3001 gatgacgcaa ctcatcccag atctcctggg tgttgttgta gtgcatcgga taacccatac
     3061 gagtggcaat ttcactgatg atttgccagt ccgttttcag atcccacttc ggctcaaccg
     3121 ccttgaagaa acgctggaag ccacggtcag ccgcagtaaa cacgccttca tgctcgcccc
     3181 acgacgttga cggtaaaatg acatccgccg ccgacgcggt tttggtcata aagatgtcct
     3241 gaacgatgac cagttccaga tcttcaaagg ctttacgtac tgccgacagc tccgcgtcag
     3301 tttgcagcgg atcttcgccc ataatgtacg cggcacgcac ttcgccatgc gctgcgcggt
     3361 gcggcagctc gctgatgcga tagccggtat gagcaggcag gctttccacg ccccaggctt
     3421 tggcgaattt ctcgcggtta gccggatctt tcacgtactg gtatcccgga tacgtatccg
     3481 gcagcgcgcc catatcgcag gcaccctgca cgttgttctg accacgaacc gggtttacac
     3541 ccgcatgcgg cttaccgagg ttaccggtca gcatcgcgag gctggtcaga gaacgcacgg
     3601 tttccacgcc ctggtagaac tgggttacac ccatgcccca caggatggcg gcgcttttcg
     3661 cctgggcata catccgtgcc gcctgacgaa tctcactggc gctgacgccg gtgatatctt
     3721 caaccgactc cggcgtgtag ccttcaacga ttttacgata ctcttcaaag ccttctgtac
     3781 gggaagcgac gaatgctttg tcgtacagat tttcttcaat aatgacatgg cccatcgcat
     3841 tcaacagcgc gatgttcgag ccgtttttca gtgcaatgtg catgtccgca atgcgcgcgg
     3901 tttcaatttt gcgcggatcg cagacgataa ttttcgcccc gttacgttta gcgttaatta
     3961 cgtgattcgc cacgattggg tgggaatccg ccgggttgta cccgaaaacg aacactaaat
     4021 cggtattatc aatttcgtta atagcattgc tcattgcgcc attaccgacc gattggtgca
     4081 gacctgcaac cgatgggccg tgtcagacac gagcgcagca gtcaacgtta ttggtaccaa
     4141 taacggcgcg cgcaaatttt tgcattacat agttggtttc gttacccgta ccacgcgagg
     4201 agccggtcgt ctggatggca tccggaccgt acttctcttt gatggcgctc aggcgctcgg
     4261 caacgtaatt cagtgcctca tcccaggaaa caggttcgag tttgccgcca cgctggcgac
     4321 ggatcatggg ggttttcagg cgcggggtca ggatctgggt atcgttaatg aaatcccagc
     4381 cataataacc cttcagacac agggtaccct ggttggtttt cccctgcgct gcctccgccc
     4441 ggacgatttt gccgttatcg acgaccaggt tgattttgca acctgatgcg caataggggc
     4501 aaaccgtgac gacttttttc atcggtctcg ctccagttaa tcaaatcacg catacgcgct
     4561 ctcgactaca gtatgcatct tttatgccac attttatttg gggtcattcc ctgatattac
     4621 gggcactatt tattcaaaac tctgacgaaa aacaggctgt cgtcagtttt gacgtgacga
     4681 aacgaaatac cgcgtgacag ccatcacgcg gcagacattt tattttttct cgacgacggg
     4741 acttgcctga tacccgccgc ccagcgattt catcaactga atgctttgga tcacccggcg
     4801 gctgtccagc atcagtaatg acatctcctc ggcaagcact ggcaaccggg cttcggtggc
     4861 ctgtaagcgg ctggttaagc cgcgttgata ggcggcctcg gcagcgcgct gggtgaagcg
     4921 cgttgcttcc acgcgttcag cctgcatctc tcgctcgtcg ttgagcgttt gcagacgcgt
     4981 gccgttgacg gcaacgtcgc gcaccgcatt cagcactgac tggttgtaac gttcaatcat
     5041 catgttgctg gcggcgcgag tgccttcgag attggcattc aaccgtccac cgtcaaacag
     5101 cggcaatttc agacctggga taaagttgaa ctggcgactg gtttttttga ataaggtatc
     5161 caggtggata gaatccaggc cgaaaaacgc tttgatatca aagctcggat agaacagcgc
     5221 ccgcgcggaa tccacctgat ctaatgacgc ctgaacatac cagtgcatag cttgcagatc
     5281 cgggcgtctg gcgagcaact cataagagag tgtcgccgga ataccggtct ggacttgcgg
     5341 taatgccacc ggtttgatct ccggcatatc gctggctccc gcgccaatca atgcacgcag
     5401 agattctcgc gtttcggtga tttgcccttt aacggcagca atttgtttat cgaccgccag
     5461 aatctgtgcc cgcgcgccgt ggaaaggcac ttgcgcttcc agaccgtgcg ccactttact
     5521 ctggtgcgct ttcaccgcgt aatcaatcac atcgcgtgtt tgttctaaca gatcgagcat
     5581 ctgatagctg gcctgcatac tgtaataaag ctgcgctacg cccgtggtca gcgatagctc
     5641 tactgctgcg gtttctgcca gcgcggcatt atgcgcgcca atggcggcgg caaccgctga
     5701 gcgatgcaca ccccacaaat caagatccag tccggcaaac aaaccgactg tggcttccgt
     5761 atagtacggc ccgtccatac ccagtgctgg cgcatccatc gcataagggc ttaaaaagcc
     5821 gttcgccgag acacgttggc ggttgagcat ccctaacgct gccacctgca actgtgaacc
     5881 agcatctaac aaatcggcct gggactgcgc tttttcttcc cgcagtttcg cttcggcgag
     5941 ggtgtgtgaa ccacttagcg tccgttggat cagggcatcc aactgcgggt cattgagttg
     6001 tttccaccac tgcgcctgcg gccagccgga gctggcaaga tgaatatcat cggccagttt
     6061 gatttgttcc ggtttgagct gttgatgagg tgcagaatcc ttacgtacca gggcacagcc
     6121 ggaaatcagc gtcgtgctgc cgagaatgct gcacaacagc agacgtgaaa gttgacgatt
     6181 gatcattgcg tggctccttg tgcctgttcc gtggcgggcg ttaatgccgg ggctgtccag
     6241 tctggcaagc gggcgaaaag ctggcagacg tgttgctcct gttttaataa ggtgggcagg
     6301 atggcctgcg aggcgggtgt ttcttcaagc gttatttgag gagaacggct gagtgcggtt
     6361 gccagaccgg cggcgtattt ctccagtgcg tcggcaaact gacctgctct gtccggctgt
     6421 agtgcgttat ccagcgcctg cgccgagttc caggttgcat cccaggcgtg aagaatatcg
     6481 cggccctgac gaataaccgt ttgcgaacgt tcaatcagta aagcgcgttc ttcgctgtcc
     6541 agttgacgct ccaacgccac gcgttggcac atctcttcac aggcattaaa cgccgcatgc
     6601 agacctatac gaatttgcaa ataagtgcgc agagctgtga cttcctgctg gcgtgggatc
     6661 cgcattactt tactcaacat acccagcgcg ccagccagtt tttgcggcag tgtgcgcgct
     6721 tcactttcag gccagacaaa ggtgtaaatc accgcggaca ccacggtacc aatgatgata
     6781 cccagggcgc gatcgcgaat ttccaccagg tcgtacaccg ggccaaaaac gttttcgagc
     6841 gtggcgagcg cgaaggtgac caccatctgt gtgccgatat aagaagagcg ttcagagctg
     6901 gtggcaatcc atgcgcccaa cagaaaaatc ggtgccagca caaatagcaa ttcgacaata
     6961 ttgtccagcc agggcatgac cagtaacgtg aataacagcg ccagaattgc accgcagaag
     7021 gcaccgccaa aacgcagcgc cattttctga tacgacgaac caacattcgg gttagcgacg
     7081 atcacgcagg tcagcataca ggtgtgaatg ccttcccagt ccacgccgct gtagaaggtg
     7141 taacagatca aacaggcgag cagcgttttt accgcgtagc gcatatagtc tggattggta
     7201 aaagcgtcgg cgaccattga tggcggtttg gtggcgggcg ttggcggcgt attcgggtcc
     7261 atctgaccca gttgcaataa cgtctggcag atattctcca ggttacattc ccgtgccgcc
     7321 atcgcttcac tttcagtgat ccgccagtcg ctttgccagc actgaccttc ggcaatggca
     7381 tgctgcagtt tgttgatttc tgaggctaat ttttgccgaa attcaataat tgcctgagaa
     7441 tcagcaaaag aggtgggatc gtagcgattc agcgtcgagt aaatgtaggt taccgttgcc
     7501 acgcagcttt gccaccatgc gctctgagtt cgccagttgg catcgtccgc gagacaaaat
     7561 acattcagtt tttgcagcgc cagcgcctct ctttcaatcc gcgtttcggg tagcggtgtg
     7621 aggctgttcg tcaggtggct aatggcatca tcaagccgat cattaagcgc ctgatgcatc
     7681 tgcgtgatag cgcggctggg aaaccacagc acgccgatta acgtcatcaa taacgtcggg
     7741 tagaggccga caacgataca ccacagggtt aagcgcacga ccacttccgg atagtcgagc
     7801 atggcgggga aggtttgccc gtaaatggcg acaatggcga cagcgaaaaa gaccagtccc
     7861 agccgatggg tgcgcatcaa aaacatgcag cccatcagga tcggcccggc gatgatcaat
     7921 cggatcaacg gttcgccgta tgaccatttg tagatcaaaa acaggctgcc gatctccagc
     7981 accgtggcaa ccacaaacaa gatcgccaca aatttggtgt aaaacgcgtt cgactgaata
     8041 ccgtaaaaca gcactgccag cgataacgcc acaaaaggga tctcaaaagt catcgagatc
     8101 agaatcacca gtaggcagcc tacccagagt tgcacggtct gcggcacacg acctggccgc
     8161 cgctcgctta actcttcatg aaagaacgcc agcagcctga ccaccggtaa tggcagggag
     8221 ttgagcgcgc tcattattgc ggctcaagat tagcgactgc cgaagtgccg atgcggaaca
     8281 tttccgggtc aggtttatca accatgattt tgaccggaaa acgctgggca acgcggaccc
     8341 agttaataga acgagacact ttcggcaggc cgcccagcac caggccgccg tcatccggta
     8401 gcacgccgta gccaatcgaa tccactttac cctcgaaggt tttgccgctg tcgctcatca
     8461 ggcgaatggt tgcgggtgta cctgagcgaa tatttttcag atcagtttcg cggaagttgg
     8521 cgatcacgta ccagtgacga gtgtcgatta gggtgaaaat agggcgcatg gcagaagcaa
     8581 attgcccgac ggaggttttg agggaaatga cccggccatc aaacggcgcg cgaacggtcg
     8641 ccatttccag atgcagtttc gtcaacgcaa tatccgcttc cactgccgca cgttgcgcca
     8701 ccagcgcatc cacgccgctg acggcgctgg cggctgactg cgcctgcaac aatccggcat
     8761 taagatcggc ttctgcggcg cgctgcgcgg ttctcgcgcg gtcaacgtct tccgctgaga
     8821 cgaaaccttc tttcagtaat ggctcggtgc ggcgtaatgt atcactggcc tgtttcgcgg
     8881 cggcacgggc tttttctacc gtggcattaa ccgagtcggc accaaactgt tgcgcgtcaa
     8941 cgctacgctg ggtgagcata atttgcttat ccagcgccgc gagggaggct tcggctttcg
     9001 ccagattggc ctcgtatggg cgcgggtcga tgcgaaacag caaatcgccc tgtttcaccg
     9061 cctggttgtc ggtgaccgcc agttctacaa tgcgaccgct gacttccggc accacatcaa
     9121 tggtatctgc tgacgcgtag gcgtcattag ttgatggcgc actgtctacg cgccagataa
     9181 cgaaaacaag ggcaaccagc gccaacgcaa ccactaacag agcagggaat ttactgcgag
     9241 gagctttttt cggcgtactt tccatgagcg cgtctcaaat agattagaaa aatgccagcc
     9301 agagcagcat ggcgtagaga gcaaaaaggg cggtataaat aattccgaca aatgccagat
     9361 tgatatttgc ccgctgaata attcgcctcg tgatgagcgt caaaataagg ctggcaatgg
     9421 cgcagaaaaa ccagctggga taataagcgc cgatcaccgg gattgccgga gataatgagc
     9481 atcccgaaac catgaagacg ggaattatcc aggctgtttt tttgagttgc atcgccatgc
     9541 gagagagaac tgttggcata ttaaatacat cctgtctgct gcgaacaata tctgtagtga
     9601 atatcaatat ccattgaagg cgtcagtatg gctattttct cgatgaataa aaatagcttt
     9661 attcagccac cgcattca
//
LOCUS       DACBVI010000164         9123 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.165, whole genome shotgun sequence.
ACCESSION   DACBVI010000164 DACBVI010000000
VERSION     DACBVI010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.165"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            305..826
                     /locus_tag="IU325_19375"
     CDS             305..826
                     /locus_tag="IU325_19375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP5811166.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            823..1776
                     /gene="fecR"
                     /locus_tag="IU325_19380"
     CDS             823..1776
                     /gene="fecR"
                     /locus_tag="IU325_19380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HAP5811167.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1863..4187
                     /gene="fecA"
                     /locus_tag="IU325_19385"
     CDS             1863..4187
                     /gene="fecA"
                     /locus_tag="IU325_19385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HAP5811168.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLTSRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            4232..5134
                     /gene="fecB"
                     /locus_tag="IU325_19390"
     CDS             4232..5134
                     /gene="fecB"
                     /locus_tag="IU325_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HAP5811169.1"
                     /translation="MLAFIRFVFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            5131..6129
                     /gene="fecC"
                     /locus_tag="IU325_19395"
     CDS             5131..6129
                     /gene="fecC"
                     /locus_tag="IU325_19395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HAP5811170.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            6126..7082
                     /gene="fecD"
                     /locus_tag="IU325_19400"
     CDS             6126..7082
                     /gene="fecD"
                     /locus_tag="IU325_19400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HAP5811171.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            7083..7850
                     /gene="fecE"
                     /locus_tag="IU325_19405"
     CDS             7083..7850
                     /gene="fecE"
                     /locus_tag="IU325_19405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HAP5811172.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNAGVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(8407..8664)
                     /locus_tag="IU325_19410"
     CDS             complement(8407..8664)
                     /locus_tag="IU325_19410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811173.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(8747..9123)
                     /locus_tag="IU325_19415"
                     /pseudo
     CDS             complement(8747..9123)
                     /locus_tag="IU325_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT         1989 a         2604 c         2515 g         2015 t
ORIGIN      
        1 aaatggaagc cacgcaagca ccttaaaatc accatcatac actaaatcag taagttggca
       61 gcattaccga aatccccatc ctttagggca gtgaggatgt caataatagc agaccacatc
      121 atgagtcatt ctgaccgaca catgccagaa gcagaggctc catcccattt tatacctacc
      181 ttataacact tagaaaaaca acatgttaaa aatgtctatt ggaaacaatt ttatttccaa
      241 ttgtaatgat aaccattctc atattaatat gactacgtga taattaactt ttgatgcact
      301 ccgcatgtct gaccgcgcca ctaccacagc ttccttaacg ttcgagtcgc tttatggcac
      361 acatcacggc tggttgaaaa gctggctgac gcgcaaactc cagtctgctt ttgatgcaga
      421 tgacattgcc caggacactt ttttgcgggt aatggtcagc gaaacgctct cgacgatccg
      481 cgatcctcgc tccttcctct gcactatcgc caaacgcgtg atggtggacc tgtttcgccg
      541 aaacgcgctg gaaaaagcgt atctggagat gctggcgctt atgccggagg ggggagcgcc
      601 ttcacctgag gaacgcgaaa gccaactcga gaccctacaa ctcctcgaca gcatgctgga
      661 cgggctaaac ggcaaaacac gtgaagcgtt tctgctttcg caactggatg gtctgacata
      721 cagcgagatt gcgcacaaac tcggtgtttc catcagctcc gtgaaaaaat acgtggcgaa
      781 agccgtcgag cactgcctgc tgttccgtct ggagtatggg ttatgaatcc tttgttaacc
      841 gattcccgcc gtcaggcgct gcgttcagct tcccactggt atgccgtgct aagcggcgag
      901 cgcgtcagcc cacaacagga agcgcgctgg caacagtggt atgaacagga tcaggataac
      961 cagtgggcct ggcagcaggt tgaaaacctg cgcaaccagc ttggcggtgt gcctggcgac
     1021 gttgccagcc gggcgttgca cgatacccgc cttacccgcc gtcacgtgat gaaaggatta
     1081 ctgctgttgc tcggcgctgg cggaggctgg cagctctggc agtcggaaac cggcgaaggt
     1141 ctgcgggcag attaccgcac cgccaaaggc acagtcagcc gtcagcaact ggaagatggc
     1201 tccctgctca cgctgaatac ccaaagcgcg gcggatgtgc gttttgatgc gcatcagcgc
     1261 accgtccggc tctggtacgg tgaaatcgcc attactaccg cgaaagatgc cctgcaacgc
     1321 cccttccgtg tcctgacccg tcagggccag ctcactgctt tagggacaga atttaccgtc
     1381 cgccagcagg ataatttcac gcagcttgac gttcagcagc acgctgtgga agtgcttctc
     1441 gccagtgccc ccgcgcaaaa acgcatcgtg aacgctggtg aaagcctgca gttcagcgcc
     1501 tctgagtttg gcgcagtgaa accgctggat gacgagagta caagctggac gaaggacatc
     1561 ctgagcttca gcgataaacc actgggtgag gtgatagcca cgctaacccg ttaccgcaac
     1621 ggcgtgctgc gctgcgatcc cgccgttgcc gggctgcgcc tgagcgggac gttcccgctg
     1681 aaaaataccg atgcgatcct gaacgttatc gcgcaaacgc ttcccgttaa aattcagtct
     1741 attacgcggt actggataaa catttcacca ctgtaaggaa aataattctt atttcgattg
     1801 tcctttttac ccttctcgtt cgactcatag ctgaacacaa caaaaatgat gatggggaag
     1861 gtatgacgcc gttacgcgtt tttcgtaaaa caacaccttt ggttaacgcc attcgcctga
     1921 gcctgctgcc gctggccggt ctctcgtttt ccgcttttgc tgcacaggtt aatatcgcac
     1981 cgggatcgct cgataaagcg ctcaatcagt atgccgcaca cagcggattt accctctcgg
     2041 ttgacgccag cctgacgcgc ggcaagcaga gcaacggcct gcacggcgat tacgacgtcg
     2101 agagcggcct gcaacaactg ctggacggca gcggactgca ggtaaaaccg ctgggaaata
     2161 atagctggac gctggagccc gcgcccgcac caaaagaaga tgccctgacc gtggtcggcg
     2221 actggctggg tgatgcgcgt gaaaacgacg tatttgaaca tgctggcgcg cgtgacgtga
     2281 tccgccgtga ggatttcgcc aaaaccggcg caaccaccat gcgtgaggta cttaaccgca
     2341 tccctggcgt cagcgcgccg gaaaacaacg gcaccggcag ccacgacctg gcgatgaact
     2401 ttggcatccg gggcctgaac ccgcgcctca ccagccgctc gaccgtcctg atggacggca
     2461 tccccgtccc cttcgcccct tacggtcagc cgcagctttc actggctccc gtttcgctcg
     2521 gcaacatgga tgccattgac gtggtacgcg gtggtggtgc ggtgcgttac ggaccgcaga
     2581 gcgtgggcgg cgtggtgaac tttgttaccc gtgccattcc gcaggacttt ggtatcgagg
     2641 cgggcgtgga aggtcagctc agcccaacct cttcacaaaa caacccgaaa gagacgcaca
     2701 acctgatggt gggcggcaca gcggacaacg gttttggcac cgcgctgctc tactccggca
     2761 cgcgcggcag tgactggcgc gagcacagcg ccacccgcat cgacgacctg atgctgaaaa
     2821 gcaaatatgc gccggatgag gtgcacacct tcaacagcct gctgcaatat tacgacggtg
     2881 aagccgacat gcccggtggc ctgtctcgcg cggattacga cgccgatcgc tggcaatcca
     2941 cccgcccgta tgaccgcttc tggggtcgtc gcaagctggc gagcctgggc taccagttcc
     3001 agccagacag ccagcataaa ttcaacattc aggggttcta cacccaaacc ctgcgcagcg
     3061 gctacctgga gcaaggcaaa cgcatcaccc tctcgccgcg taactactgg gtgcgcggta
     3121 ttgagccacg ctacagccag atctttatga tcggcccttc cgcgcacgaa gtgggcgtgg
     3181 gctatcgcta tgtgaatgaa tcaacgcatg aaatgcgtta ctacaccgcc accagcagcg
     3241 ggcagttgcc gtccggctca agcccttacg accgcgatac gcgttccggc accgaggcgc
     3301 acgcctggta tctggatgac aaaatcgaca tcggcaactg gaccatcacg ccgggtatgc
     3361 gtttcgaaca tatcgagtca taccagaaca acgccatcac aggcacgcac gaagaagtga
     3421 gctataacgc accgcttccg gcgttgaacg tgctctatca cctgactgac agctggaatc
     3481 tttatgcaaa cactgaaggc tcgttcggca ccgtacagta cagccagatt ggcaaggctg
     3541 tgcaaagcgg caatgtggaa ccggaaaaag cgcgaacctg ggaactcggt acccgctacg
     3601 acgacggcgc gctgacggcg gaaatggggc tgttcctgat taactttaac aatcagtacg
     3661 actccaacca gaccaacgac accgtcactg cacgtggcaa aacgcgccat accgggctgg
     3721 aaacgcaggc acgttacgat ctgggtacgc taacgccaac gcttgataac gtttccatct
     3781 acgccagcta tgcgtatgtg aacgcggaaa tccgcgagaa aggcgacacc tacggcaatc
     3841 tggtaccatt ctccccgaaa cataaaggca cgctgggcgt ggactacaag ccaggaaact
     3901 ggacgttcaa tctgaacagc gatttccagt ccagccagtt tgcggataac gccaatacgg
     3961 tgaaagagag cgccgacggc agtaccggcc gcattcccgg cttcatgctc tggggcgcac
     4021 gcgtggcgta tgactttggc ccgcagatgg cagatctgaa cctggcattc ggtgtgaaaa
     4081 acatcttcga ccaggactac ttcatccgct cttatgacga caacaacaaa ggcatctatg
     4141 caggccagcc gcgcacgctg tatatgcagg ggtcgttgaa gttctgatgt gttaacgccc
     4201 ggcttgccgg gcttttagct ggaatgtgat tatgttggca tttatccgtt ttgtttttgc
     4261 aggcctgctg ctggtgatca gccacgcctt tgccgccacg gttcaggacg aacacggcac
     4321 gtttacactc gaaaaaacgc cacaacggat tgtggtgctg gaactctcgt tcgccgatgc
     4381 gctggccgcc gtggacgtca gcccgatcgg tattgccgac gataacgatg caaaacgcat
     4441 cctgcccgaa gtgcgtgcgc acctgaaacc gtggcagtcc gtcggaacgc gcgcgcagcc
     4501 gagcctggaa gccattgccg ctctgaaacc agacctgatc attgccgaca gcagtcgcca
     4561 tgcgggggtt tacatcgcct tgcagcaaat cgcgccggta ctgctgctta agtcccgcaa
     4621 cgaaacctac gctgaaaatt tgcaatctgc ggctatcatc ggcgaaatgg tgggtaaaaa
     4681 gcgagagatg caggcacgcc tggaacaaca taaagagagg atggcgcagt gggccagcca
     4741 gcttcccaaa gggacacgcg tggcctttgg cacatcacgc gaacagcaat tcaacctgca
     4801 tactcaggag acctggaccg gcagcgtgct ggcctctctg gggctgaacg ttcccgctgc
     4861 gatggcgggt gcgtccatgc cgtccatcgg cctggagcaa ctgctggcgg tcaatcctgc
     4921 ctggctgctg gttgcccact atcgcgaaga gagcattgtt aaacgctggc aacaagatcc
     4981 gctctggcag atgttaaccg ccgcgcagaa gcagcaggtt gcttcggtcg acagtaacac
     5041 ctgggcgcgg atgcgcggta tttttgctgc agagcgtatt gccgctgaca cggtaaaaat
     5101 cttccaccat cagccgctta ccgttgtgaa atgaccgcga taaaacaccc ggtgctgctg
     5161 tgggggcttc ccgttgcagc acttattatt attttctggc tgagtctgtt ttgctactcg
     5221 gccattcctg tttccggagc agatgcaacc cgcgccctgc tgcctggaca cacgccaacg
     5281 ctaccagaag cgctggtgca aaaccttcgt ttgccacgaa gcctggtcgc cgttctgatc
     5341 ggcgcaagcc tggcgctcgc gggcacgctg ctgcaaaccc tgacccacaa cccaatggcc
     5401 tctccttcac tgctcggcat taacagcggc gcggcgctgg ctatggcgct taccagcgcg
     5461 ctgagtccga cgccgattgc aggctattct ctgtcgttca tcgcggcatg cgggggcggc
     5521 gtgagctggc tgctggtcat gaccgcagga ggcggatttc gtcataccca tgacagaaac
     5581 aaactgatcc tcgcgggtat cgcgctgtcg gccttttgta tgggccttac ccgcatcacc
     5641 ctgctgctgg ccgaagatca tgcttacggc atcttttact ggctggcagg cggagtgtcc
     5701 cacgcccgct ggcaggatgt ctggcagctc ttgccggtgg tggtcactgc agtccctgtc
     5761 gtgttgctgc tggcgaatca actgaacctg ctcaacctca gcgacagcac cgcccatacg
     5821 ctgggagtga acctgacgag gctacgtttg gtcatcaata tgttagtgct gcttctggtt
     5881 ggtgcgtgcg tcagcgtggc aggtccggtg gcgtttatcg gtctgctggt gccacatctg
     5941 gcgcgcttct gggcaggctt cgatcagcgc aacgtactgc cagtgagcat gctgctgggg
     6001 gccacgctga tgctgctggc agatgtactc gcacgcgcac tggccttccc cggagatctg
     6061 cccgcaggcg cagtgctggc gctgattggc agcccttgct ttgtctggct tgtgaggagg
     6121 cgaggatgaa aattgcgctg gttattttca tcacccttgc cctggcgggc tgtgcgctgt
     6181 tatcactcca tatgggagtg atccccgtgc cgtggcgcgc gctgctgacc gactggcagg
     6241 ccggacgcga gcattattat gtattgatgg agtaccgact gccgcgcttg ctgctggcac
     6301 tgtttgtcgg tgcagccctc gccgtggcgg gcgtgctgat acaggggatt gtgcgcaacc
     6361 ctctggcatc accggatatt ctcggtgtta accatgccgc cagcctggcc tctgtggggg
     6421 ctctacttct tatgccgtca ctgcccgtga tggtgctgcc gctgctggcc tttgcgggcg
     6481 gcatggcggg gttgatattg ctgaagatgc tggcaaagac ccaccagccg atgaagctgg
     6541 cgctcaccgg cgtggcgctt tctgcatgct gggccagcct gacggattat ctgatgctct
     6601 cgcgcccgca ggatgtgaac aacgccctgc tgtggctgac cggcagctta tggggccgtg
     6661 actggagctt tgtgaagatt gccatcccgc tgatgatttt atttctgccg ctgagcctga
     6721 gtttttgccg cgatctcgac ctccttgcac tcggcgatgc gcgcgccacc acgctcggtg
     6781 tgtcggtgcc ccatacccga ttctgggctt tgttactagc tgtcgccatg acatctaccg
     6841 gcgtggccgc ctgcggcccg attagcttta ttggtctcgt ggtgccgcat atgatgcgta
     6901 gcatcaccgg tggacgtcac cgcagactgc tgcctgtttc ggccctgaca ggtgcgttgc
     6961 tgttggtggt tgccgatctg ctggcgagaa ttattcatcc cccactggag ctcccggttg
     7021 gcgtgctgac cgccattatc ggtgcgccgt ggtttgtctg gttgcttgtg agaatgcgat
     7081 aaatgacttt acgaactgaa aatctgacgg tcagttacgg gacagacaag gtacttaacg
     7141 acgtttcact ctcactgcca acggggaaga tcaccgccct gatcggtcct aacggttgcg
     7201 ggaaatcgac gctgttaaac tgtttttcgc ggcttttaat gccgcagtct ggcaccgtat
     7261 ttctcggcga taatcccata aatatgctct catcgcgcca gttggcccgc aggctttcgc
     7321 tgctgcctca gcaccattta acgccagagg ggatcacagt ccaggagctg gtttcgtatg
     7381 gtcgtaatcc ctggctgtca ctctgggggc gtctctccgc tgaagacaat gcaggagtta
     7441 atgtcgccat gaaccagacc cggatcaatc atcttgccgt tcgtcggtta accgagcttt
     7501 ccggcggtca gcgccagcgc gcatttctgg caatggtcct ggcccagaat acgcccgttg
     7561 tattacttga tgagccaacc acctatcttg atatcaatca ccaggtggac ctgatgcggt
     7621 tgatgggcga actccggact caggggaaaa cggtggtcgc tgtgctgcac gaccttaatc
     7681 aggctagccg gtactgcgat caactggtgg taatggcaaa cggacatgtt atggcgcaag
     7741 gcacaccaga agaggtgatg accccaggat tgctgagaac agtattcagc gtggaagcgg
     7801 aaatacaccc cgagccggta tctggcaggc cgatgtgcct aatgaggtag attgcacagg
     7861 ccgtaagaac caaaccacga ctgaatgaaa ctggactggc gccagcaagc ctgttcagac
     7921 tggggctgaa cttttccgga ctctgaaaga ttaccaatac tcatcgtcca tccgcttgct
     7981 ttaggctgac aggttcataa tcaacgcaaa ccagagctgt acaggcttgg gcgcggcttt
     8041 caaaccagtc gtgatcacgg caatcaattt tgaactctgc ttaacggaca tttctgtata
     8101 acccttacgg caacgaaaaa cgcgaagtta aaattttaga aacccaaaaa cgtgacatga
     8161 ctaagtttag atttcagggg ggagatcaaa aaatttcgct ctgtgccaga gcggacattc
     8221 acggagctgg ttcattacca atgaggttgg gcttttgagg ataaatcaat gatcagacgc
     8281 caacgtaaat caaaagcaac cctggaacgg aattgctaat ccagtttctg accatcgatt
     8341 tttctaaaaa gtgtgcgttt gctactactt aggtaggtgc agctttctta atcaccggca
     8401 gccacgttat acaggccagt tgatggatcg attgttatca atgatatctt catgagtcgg
     8461 tgtctcaccc agcttaccga agctggcata aagtgaaggc agacgggccc gtccttctcc
     8521 ctttttcgcc agagggaaag cgcgaagcat ggtggcagcc tcccagtcac aacaatagga
     8581 tggtgtgcac ggctgctgac gccatgattc agcgatagag ccggaaaata cggggtcaaa
     8641 gccggtatca ttaaccagag tcatcgtgac ctgtactgcg gctggatgat ctcctgctac
     8701 tgcaactgcc aggcgaccgc ggctcccttc aggtagtctg ttgtggtcaa ctaaaactgg
     8761 cctccgcgtt agagtttttc cagtatcggt tttctgattc gtttggtggt aacccaccat
     8821 tatattcgtg cggtcttagt gcgctgtaat atccaacgat atagtccgtt attgcgtgag
     8881 ctgcatcgct gaagcttaca tagcccgtcg ctggcaccca ttcgttcttc agactcctga
     8941 agaagcgctc catggggctg ttatcccagc agtttccacg ccgactcata ctctgcctga
     9001 tccggtatcg ccatagtaac tgccggaact gcctgctcgt ataatgactg ccttgatcgc
     9061 ttggaacatc accccgacgg gcttaccacg ggtttcccat gccatttcca gtgctttcat
     9121 ggt
//
LOCUS       DACBVI010000165         9064 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.166, whole genome shotgun sequence.
ACCESSION   DACBVI010000165 DACBVI010000000
VERSION     DACBVI010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.166"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..1182
                     /locus_tag="IU325_19420"
     CDS             <1..1182
                     /locus_tag="IU325_19420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP5811174.1"
                     /translation="QDNTGAVPADEADREQPEETAETVSPVQRLAQLTAELDALHNDR
                     LLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKLLGDTIKGQDLAIASLHK
                     HLLTARADLRLPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTINMSEFQEK
                     HTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQSFDKGE
                     MADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEELYPVLADFFKPALLARME
                     VVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVVIEPEVTDEIMSRVTRAENGARM
                     LESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDDAFTADVEDAQNDESVTKDETV
                     L"
     gene            1179..1922
                     /gene="tagO"
                     /locus_tag="IU325_19425"
     CDS             1179..1922
                     /gene="tagO"
                     /locus_tag="IU325_19425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HAP5811175.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAETSSMAGVAVAVATTT
                     PPDATATSQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     LEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRVACHWAGE"
     gene            1927..3339
                     /gene="tssA"
                     /locus_tag="IU325_19430"
     CDS             1927..3339
                     /gene="tssA"
                     /locus_tag="IU325_19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HAP5811176.1"
                     /translation="MAMDLRDPNVWITHLLENLPEEKLASALKDDNPDWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWSVAAPQNMAHKKRFASQVIKRFETGIEGFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELALATDDLFALYQRAFNRAAPAPAPTPAASDSSRQTTATSESGVTQPSAPAPQIA
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQSITSTPQAESDGRTPLAA
                     VSADMVADYQSRLASADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADTIR
                     DEVIRFLERLPQLTGLLFNDRTPFLSEQTKQWLAASPDGKVAPVAQIGEESQAARACF
                     AGQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLIQLAQQQYRMLLMIGSQMM
                     VSDWEPSLLTQLEQKFTAEQ"
     gene            3448..6882
                     /gene="tssM"
                     /locus_tag="IU325_19435"
     CDS             3448..6882
                     /gene="tssM"
                     /locus_tag="IU325_19435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HAP5811177.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQQLEKMQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQSLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLMRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLAEMGLYQGRR
                     IGPYVERTYLQLLEQRYLPSLFNGLVKEMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGAFSEKLSA
                     KERDEALAEPDYQLLTRLGHEFAPENSTLTVQKDKESTMQAVYLQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMSKTLPAPLNRWAGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPHSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLVGARGGAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            6893..8245
                     /locus_tag="IU325_19440"
     CDS             6893..8245
                     /locus_tag="IU325_19440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811178.1"
                     /translation="MNSNGLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAEPDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQPKSNVENAVQTTVRTLIYAPETTASSTPEAVVPPLPDLPEMK
                     VEVRSLSENPPQASVMKQGSTVRGFITGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLGVSPVQPLETGMQMMRVADSRWPESLQQQQASTH
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMRREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYQDTRAQKQNIDALEKQINERLEGVLIRWLLLKNNVMPEAATGTTAEK
                     "
     gene            8269..8751
                     /gene="hcp"
                     /locus_tag="IU325_19445"
     CDS             8269..8751
                     /gene="hcp"
                     /locus_tag="IU325_19445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HAP5811179.1"
                     /translation="MANMSYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMVTCEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            8795..>9064
                     /locus_tag="IU325_19450"
     CDS             8795..>9064
                     /locus_tag="IU325_19450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811180.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRRQNTHHVILLTLDVAKKILSSITSFDYIHFITTHPSGIKDT"
BASE COUNT         2151 a         2504 c         2555 g         1854 t
ORIGIN      
        1 caggataata ccggtgccgt accggctgat gaggccgaca gagaacagcc ggaagagacc
       61 gctgaaacag tttccccggt acagcggctg gcccagctca ctgccgaact ggacgccctg
      121 cataacgacc ggttgctggt ctccccgcac gtcgataaaa aacagattgc ggcggtgatt
      181 gccgaatgga ccggcgtgcc gctcaaccgc ctgtcgcaga atgaaatgtc ggtcatcacc
      241 gacctgccga aattgctggg cgacaccatc aaaggccaag acctggcgat tgccagcctg
      301 cataagcatc tactgaccgc acgcgccgac ctgcgtcttc cgggacgccc gctcggtgcg
      361 ttcctgctgg ctggccccag cggtgtgggt aaaaccgaaa ccgtcctgca actggcagag
      421 ctgctctacg gcggtcgcca gtacctgacc accatcaata tgtccgaatt ccaggagaaa
      481 cacaccgtct cgcggctgat tggttcgcct ccgggctacg ttggctacgg tgaaggcggc
      541 gtactgaccg aagcgattcg ccagaaaccc tactcggtag tactgctcga tgaagtggaa
      601 aaagcgcacc cggatgtgct caacctgttc taccagtcgt tcgataaggg cgaaatggca
      661 gacggtgaag gccgcctgat tgactgcaaa aatatcgtct tcttcctgac gtccaacctc
      721 ggctaccagg taatagtcga gcacgccgat gacccggaaa ccatgcagga agaactgtat
      781 ccggtgctgg ccgacttctt caaacctgcc ctgctggcgc gtatggaagt ggtgccgtat
      841 ctgccgctgt cgaaagagac gctcgccacc attatcgccg gaaaactggc ccgtctggat
      901 aacgtgctgc gcagtcgctt tggtgcagaa gtggtcattg aaccggaagt gacggacgaa
      961 atcatgagcc gcgtcacccg cgcggaaaac ggcgcgagga tgctggaatc ggtcatcgat
     1021 ggcgacatgc taccgccgct ctcgctgctg ctgttgcaga aaatggcggc taacacggcg
     1081 attgcccgga ttcggttgtc ggcggtggac gacgcattta cggcagacgt ggaagatgct
     1141 cagaacgacg agtccgtcac aaaggatgaa acggttttat gagcaggttg cgcgttttcc
     1201 ggcagacagg atggtttatc gcgggcctga tgacgggctt acccgccact gccgcgcccg
     1261 cagaaacatc gtcaatggcc ggtgtggcgg tcgccgtcgc caccacgacc ccgccggatg
     1321 cgacagccac gtcacaggcc atgcagtcct gtcggcggga gtccgcggcg ctggagcggc
     1381 ttgactgcta tgaccgcctg ctggcaccgc tgtccccgtc agggtttgac ggggcgctgg
     1441 tcaaagccgg ttttgtgggc gaggcctgga cccgcgccac agagctggaa aaacgccgcg
     1501 agggcaacac cacagaactg ctggtgacgc aggtgccggg ggagcgcccg acggtggtga
     1561 taaccacccc ggctatcggc catgttccgc cgcgcccggt gctgatgttc agttgcgtgg
     1621 acaatatcac ccggatgcag gtggcgctga tgcaccctct ggatgtccat gatatcgccg
     1681 tcactctgaa tgccgacagc cgggcattgc gtagccactg gtttgtgcgc gagaacggca
     1741 ccctgctgga gtccagccgg gggctgtccg ggattgatga aattaagcaa ctgttcggcg
     1801 caaaaacgct gacagtggat accggcgcag ataacgccgc aggaaagctg acctttaaca
     1861 ttgacgggct ggcaagggcg attgcccccc tgcgcgttgc ctgccactgg gcgggagaat
     1921 aaaaatatgg caatggactt acgcgatccg aatgtctgga taacgcacct gctggaaaac
     1981 ctgccggaag aaaaactggc atcggcgctg aaagatgaca acccggactg ggagtatatc
     2041 gacggcgaaa tcgtcaaact gggatctctg gcccacagtc aactggatat cccggaactc
     2101 cagcgccggg ggctggtcat tctggcttcg gagagtaagg actttcgtct gctggcgcat
     2161 ctgctgagaa ccctgcaaca cgccggtgat cctcatctcg cattgcgcct gctggcgctg
     2221 tatgtggagc attactggtc ggtggctgcg ccgcagaata tggcacataa aaaacgcttt
     2281 gccagccagg tcattaaacg cttcgaaaca ggtattgaag gcttttcaca gaacgccgcc
     2341 acagcgcagc gcgacgcgct gtcaggggaa ctggcgaaac tggcgcagtg ctggcagtca
     2401 cataacgccc cggaactggc actggctacc gatgatcttt ttgccctgta ccagcgtgcg
     2461 tttaatcgtg cggcaccggc tcctgccccc actccagcgg cctccgatag ttcacgacag
     2521 accaccgcca cgtctgaaag cggcgtgacg caacccagtg ctccggctcc ccaaatcgcc
     2581 atcgacagtc acgacgacaa agcctggcgc gacacgctgt taaaagtggc ggctatttta
     2641 tgtgaacgcc agccggactc gccgcagggc tatcgcctgc gccgccatgc cctgtggcaa
     2701 tccatcacca gtacacccca ggcggaaagc gatggacgta ccccattggc tgcggtctct
     2761 gccgatatgg tggcggatta ccagtcccgg cttgccagcg cggatatggc gctgtggcag
     2821 caggttgaga aaagcgtatt gctggctcct tactggctgg acggtcactg tctttctgca
     2881 cagacggcac tgcgtctggg ttacaaacag gtggcagaca ccatccgcga tgaggtcatc
     2941 cgcttccttg agcgtctgcc ccagcttacc gggctgctgt ttaatgaccg cacaccgttt
     3001 ctcagtgagc agacgaaaca atggctggca gcttcgcccg acggcaaagt tgcaccggtt
     3061 gcgcaaatcg gtgaggaatc gcaggcagcc agagcctgtt ttgctgggca gggtctggag
     3121 gcggcgctgc gatatctgga catgctaccc gaaggcgatc cccgcgatca gtttcaccgc
     3181 cagtaccttg ccgcacagtt gacggaggag gcggggctga tacagcttgc gcagcaacag
     3241 taccggatgt tgttgatgat agggagtcag atgatggtgt ctgactggga gccatcatta
     3301 cttacgcagc ttgaacaaaa attcacggca gaacaataaa agagaacagg agtttttgtg
     3361 ttcagattac caacaccccg actactcagc ggactcaaat cagccctacg cccggcgatg
     3421 ccccggttta aagtctccgc tttctggctg ctgatactgg cgtggatttt tctgctggta
     3481 tggatctggt ggaaaggccc gacgtggacg ctgtatgaag aacagtggct caaaccactg
     3541 gcgaaccgct ggctggcaac ggcggcatgg gggattatcg ccctagtgtg gcttaccgtc
     3601 cgggtgatga agcgcctgca acagcttgaa aaaatgcaaa agcagcagcg cgaggaggcc
     3661 attgatccgc tcagtgtgga gctgaacgcc cagcagcgtt accttgaccg ctggctgctg
     3721 cgcctgcaac gccatctcga caaccgccgt ttcctgtggc agttgccgtg gtatatggtc
     3781 atcggcccgg cgggcagtgg caaaacgaca ctgctgcgcg agggttttcc gtccgacatt
     3841 atttatgccc cggagggcgc acgcggcgca gaacaacgcc tgtatctcac gccccatgtc
     3901 ggtaaacagg cggtgatctt tgatatcgac ggcacactct gcgctcccgc tgatgcggat
     3961 atcctgcatc gccgtctgtg ggaacatgcc ctcggctggc tgaaagaaaa gcgcgcgcgc
     4021 cagtcgctga acgggattat tctgacactc gatttacccg atctgcttac cgcagacaaa
     4081 cgccgccgcg aacatctgtt acagacgctg cgcagccgct tgcaggatat acgccagcat
     4141 cttcactgcc agttaccggt ttacgtggta ctcacccggc ttgatttatt gcagggtttt
     4201 gccgccctgt tccagtcact gaacagacag gatcgcgatg caattctagg ggtcacgttc
     4261 acccgccgcg cccatgaaaa tgatgactgg cgaacagagt tgaatgcttt ctggcagaca
     4321 tgggtggatc gaatgaacct agcgttgccg gatctgatgg tcgctcagac tcacacccgc
     4381 gcgtctttat ttagtttttc ccgccagatg cagggaagcc gtgaaccact ggtgtcactg
     4441 cttgagggtc tgcttgacgg cgaaaatatg aacgtgatgc tgcgtggcgt ctatctcacc
     4501 tcttcgcttc agcgcggaca gatggatgat attttcaccc agtctgccgc ccgccaatac
     4561 cggctgggca ataacccact ggcgtcctgg cctctggtgg acaccgcacc ctatttcacc
     4621 cgtagcctgt tcccgcaggc gttactcgca gagcctaatc tggcaacgga gagccgcgcc
     4681 tggctgatgc gttcccgtcg ccgcctgacg gttttctctg ccactggcgg cgtggcggca
     4741 ctgctgctca tcaccggctg gcatcactat tacaatggta actatcagtc cggcatcacc
     4801 gtgcttaagc aggccaaagc ctttatggat gtgccgcctc cgcaggggga agatgactat
     4861 ggcaacctgc aactgccgct gctgaacccg gtacgcgatg ccactctggc ctacggtgac
     4921 tggggcgacc gcagccgcct tgcggagatg ggattatatc aggggcgacg catcgggcct
     4981 tatgtggaac ggacctacct gcaactgctg gagcaacgct atcttccgtc gctgtttaac
     5041 gggctggtca aagagatgaa cgccgcgcca ccggagagtg aggaaaaact cgccgtgctg
     5101 cgcgtgatgc gaatgctgga ggataagagc ggacgtaaca atgaggtggt gaagcagtat
     5161 atggcaaaac gctggagcga aaagttccac ggccagcgcg atatccaggc gcaactgatg
     5221 tcccatcttg actacgcgct ggctcacaca gactggcatg cagagcgtca ggcgggcgac
     5281 ggtgacgcta tcagccgctg gacgccgtat gacaagcccg ttgtgtcagc acagaaagaa
     5341 ctaagcaaac tacctgtcta ccagcgagtt taccagagcc tgaaaacgcg ggcgctgggc
     5401 gttcttcctg ccgacctcaa tctgcgtgac caggtagggc caacctttga ccaggtgttt
     5461 acatctgccg atgacaataa actggttgtt ccacagtttc ttacccgtta cggcctgcaa
     5521 agctattttg taaaacagcg cgatgaactg gttgaactga cggcgatgga ttcctgggta
     5581 ctcaacctca cccgcagcgt gaaatacagt gacgccgacc gggcggaaat ccagcgccag
     5641 ttgaccgagc agtatatcag cgactacacc gccacctggc gggctgggat ggacaatctg
     5701 aatatccgca attttgagtc catcggacaa ctgaccgggg cgctggagca ggttatcagc
     5761 ggtgaccagc ctttgcagcg ggcgctgacc gtgctgcgtg acaacacaca accaggcgcc
     5821 ttttctgaaa agctatctgc taaagaacgg gatgaggccc tggctgaacc agactaccag
     5881 ttactcaccc gccttgggca tgagttcgcc ccggaaaaca gtaccctgac agtccagaaa
     5941 gataaagaaa gcacgatgca ggccgtgtac ctgcagctca ccgagttaca ccgctacctg
     6001 ctggcgatcc agaacgcgcc tgtacctggg aaatcggcac tgaaagccgt gcagttacgg
     6061 cttgatcaga acagcagcga tcccatattc gccacccgcc agatgtcaaa aacactgcct
     6121 gctccgctca accgctgggc aggcaggctg gctgaccagg cctggcatgt ggtgatggtg
     6181 gaagccgttc actatatgga agtggactgg cgcgacagcg tggtgaaacc gtttaacgag
     6241 caactggcaa ataactatcc gtttaatcca cattctgcac aggatgcctc actggatgcc
     6301 ttcgaacgat tctttaaacc agatggcata ctggatacct tctaccagca gaacctaaag
     6361 ctgtttatcg acaatgacct gagtctggag gatggcgata acaacgtcat tattcgcgaa
     6421 gatattattg cgcaactgga aaccgcgcag aaaatccgtg acatattctt cagcaaacag
     6481 aacggtctgg gaacatcctt tgccgtggaa acggtatcgc tttccggcaa caaacgccgc
     6541 agcgtactga accttgacgg tcagttagtc gattacagcc agggccgtaa ctataccgcc
     6601 catctggtct ggcctaataa tatgcgcgaa ggcaacgaaa gcaaactgac gctggttggt
     6661 gcccgcggcg gcgcaccgcg cagtatcagc ttcagcgggc cgtgggctca gttccgcctg
     6721 ttcggggccg gacagctgac cggagtacag gatggcaact ttaccgtgcg ctttagtgtg
     6781 gacggtggcg caatgaccta ccgcgtgcat accgacacgg aagataaccc gttcagcggt
     6841 gggttattca gccagtttgg cctgtcagac acactgtact gaggaggcag ggatgaatag
     6901 taacggactg acacaaacta tcgttaccgg cagtgacccg cgcgggctgc cggaattcag
     6961 cgccatccgc gaggaaataa acaaagccag ccatccgtca cagcctgagc tgaactggaa
     7021 actggtggag tcgctggcgc tggcgatttt taaagccaac ggtgtggatt tacacaccgc
     7081 cacctactat acgcttgccc ggacacggac acagggactg gcgggattct gcgaaggtgc
     7141 ggaactgctg gcggcaatgg taagccacga ctgggataaa ttctggccgc agggcggtcc
     7201 ggcgcgtact gaaatgctgg actggtttaa ctcccgaacc ggcaatattc tgcgtcagca
     7261 aatctccttt gcggaacccg atctgccgct gatctaccgc acagagcggg cattgcagct
     7321 tatctgcgac aagcttcagc aggtggagct gaagcgcgtt ccgcgcgtgg agaatctgct
     7381 ctattttatg cagaacacgc gtaagcggct tgaaccccag ccgaagagta acgttgaaaa
     7441 cgccgtacag accacggtta gaacactgat ttatgccccg gaaacaacgg cttcctccac
     7501 gccagaagcg gtagtgcctc ccctgcccga cctgcctgag atgaaagtag aagtgcgcag
     7561 tctgtcagag aatcccccac aggccagtgt gatgaagcaa ggcagtacgg taagagggtt
     7621 tatcacgggg atcgcctgtt cagtggctgt cgcctcagca ttgtggtggt ggcaggtcta
     7681 tccggtgcaa cagcaactgt tacaggttaa cgacaccgcg cagggcgcag caacggtgtg
     7741 gatggcctca cctgaactag aaaactatga gcgcaggctg caacagcttc ttggcgtctc
     7801 cccggtacaa ccgctggaaa ccgggatgca gatgatgcgt gttgccgaca gtcgctggcc
     7861 ggaaagcctg cagcagcagc aggcctcgac acattggaat gaagcactca aaacccgcgc
     7921 acagagtagc ccgcagttgc gtggctggtt gcagacccgt caggacttac atgcttttgc
     7981 agatctcgtg atgcggcgcg agaaagaggg actaaccctt tcctatatca aaaatgtcat
     8041 ctggcaggcg gagcggggac tggggcagga aacacccgtt gagtctctgt tgacgcagta
     8101 ccaggatacc cgtgcgcaga agcagaatat agatgcgctg gaaaaacaaa ttaatgagcg
     8161 actcgaaggc gtgttaatcc gctggctgct gctgaagaat aacgtcatgc cagaggcggc
     8221 aaccgggacc acggccgaaa aataattatt taattaaagg agattatcat ggcaaatatg
     8281 agttatttat ctttgagtgg agagacacaa gggctgattt cagcaggttg ctcaacgctt
     8341 gattctgtcg gaaacaaagc acagccagaa cataaagatc agatcatggt ttatgcgtta
     8401 atgcattcca tctcaagaag tcaaaatgtc aatcatcatg aattaattat caccaaaccg
     8461 gttgataaat cctcaccatt gctggcaaaa gcacttagtg acaatgaaaa aatggtaaca
     8521 tgtgaattta tactctaccg aaccagtaaa gcgggtattt accagcctta ttataaaata
     8581 aacctgtcaa aagcccgaat atcaagtatt gattttgtca caccgcatgc tgtcctggaa
     8641 aaagaactgg agccacagga gagaattgcc tttatttatg aagatatttc ctgggagcat
     8701 acgttagccg gtacgaatgc aatgagtaaa tggcaggatc gtgtacagta atttactgta
     8761 ataacataat aaattactat tgcggagaga tataatggct ggtttaagga ataataacaa
     8821 cacacagaat gcacagtggg ctgattatgt gggtgacatt cttcgggggg ctcaaccgat
     8881 aaaccagcta gtacctcagc acccttatct taacgacgtc ccactcattg atgaattaag
     8941 gcgtcagaac actcatcatg ttattctctt gacacttgat gttgcaaaaa aaatactcag
     9001 ctcaataaca tcatttgatt acattcattt tatcactaca catccttcag gtataaaaga
     9061 taca
//
LOCUS       DACBVI010000166         8913 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.167, whole genome shotgun sequence.
ACCESSION   DACBVI010000166 DACBVI010000000
VERSION     DACBVI010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.167"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..644
                     /gene="yneK"
                     /locus_tag="IU325_19455"
     CDS             <1..644
                     /gene="yneK"
                     /locus_tag="IU325_19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HAP5811181.1"
                     /translation="QFPMINPGVPIKRAHTPHIEPLHPDHHTAADYLRQFDLLVLNFI
                     SRGNFVILPRLWNNSEVHRWFGNKEPNLLTAILDITDSELKEDLLQSLMDSLGSNKHV
                     LPEVCICFLSLLAEQDFPHFQDLFLFFANMLLHYHQFMNPNESDLIDVLMPASLSDDK
                     IIKHMARKTLKLFVKNETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            794..1984
                     /gene="ydeA"
                     /locus_tag="IU325_19460"
     CDS             794..1984
                     /gene="ydeA"
                     /locus_tag="IU325_19460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HAP5811182.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGAFITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2009..2674)
                     /gene="marC"
                     /locus_tag="IU325_19465"
     CDS             complement(2009..2674)
                     /gene="marC"
                     /locus_tag="IU325_19465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HAP5811183.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            2886..3320
                     /gene="marR"
                     /locus_tag="IU325_19470"
     CDS             2886..3320
                     /gene="marR"
                     /locus_tag="IU325_19470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HAP5811184.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKRVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3341..3724
                     /gene="marA"
                     /locus_tag="IU325_19475"
     CDS             3341..3724
                     /gene="marA"
                     /locus_tag="IU325_19475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HAP5811185.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3756..3974
                     /gene="marB"
                     /locus_tag="IU325_19480"
     CDS             3756..3974
                     /gene="marB"
                     /locus_tag="IU325_19480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HAP5811186.1"
                     /translation="MKPLLSAIAAAFILFSTQGIAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4031..5470)
                     /locus_tag="IU325_19485"
     CDS             complement(4031..5470)
                     /locus_tag="IU325_19485"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP5811187.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLKFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILARGCVDFIGFSYYMSFTTQFSPDNPQLNYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(5495..7168)
                     /locus_tag="IU325_19490"
     CDS             complement(5495..7168)
                     /locus_tag="IU325_19490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008460008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HAP5811188.1"
                     /translation="MNIKTINVSLLSFSIITALFPLNALATKLTIEQRLELLENELSQ
                     NKQELRATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     SAAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFTRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDDSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVVRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            complement(7224..7535)
                     /locus_tag="IU325_19495"
     CDS             complement(7224..7535)
                     /locus_tag="IU325_19495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP5811189.1"
                     /translation="MFADEELVMELLINAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ELACLQAHKIQTALISQDEGCGKIEVNLILVHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(7563..8885)
                     /locus_tag="IU325_19500"
     CDS             complement(7563..8885)
                     /locus_tag="IU325_19500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP5811190.1"
                     /translation="MGLMTSFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNRVFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGVFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVSRSFKVLLPIFFVMVFFSALNYCLTLISPAGLN
                     DLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSF
                     IPGIFNINEPIMFGLPIVLNPIMMVPFILVPIVNCAIGYFFVSMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
BASE COUNT         2430 a         2133 c         1906 g         2444 t
ORIGIN      
        1 aacaatttcc aatgatcaac cctggtgttc ctatcaaaag agcgcatacg ccacatattg
       61 aacccctgca tccggatcac cataccgctg cagattactt acgccagttt gatttgcttg
      121 tcctgaattt tatctctcgc ggtaattttg tcatactccc ccgattatgg aataactctg
      181 aggttcacag atggttcggg aataaagaac ctaacttgct caccgctatt ctcgacataa
      241 cggacagcga attaaaagag gatttattgc aaagtctgat ggattcacta ggttctaaca
      301 aacatgtact acccgaagtc tgcatctgct ttttatccct tttagcagaa caagattttc
      361 cacattttca ggacttgttt ttatttttcg ccaatatgtt attgcactat caccaattta
      421 tgaatcccaa tgaaagtgat ttgattgacg tgttgatgcc agcatcatta agtgatgata
      481 aaattatcaa acatatggcg cgcaagaccc tcaaactttt tgtaaaaaat gaaacgccgc
      541 caaaagttac tcatgaagac ctggtgaaaa acaggcctcg ctcccctgtc agaccaccta
      601 tacccgcaac ctccaaaacg ccagacctcc ctgaacgtca ttaaaccgtg atgtgaccga
      661 ctctctgacg cgtgaacgaa tcagcgtcag agaaacggaa aacgcgatcc agatcacaaa
      721 tgcattgtat tcacatcatt aaccgtttta agatcatttc atcacttttt cgcaactcac
      781 ccgataatct gttatgacaa caaacactgt ttcccgcaaa gtggcgtggc tacgggtcgt
      841 tacgctggca gtcgccgcct tcatcttcaa caccaccgaa tttgtcccag ttggcctgct
      901 ctctgacatt gcgcaaagtt ttcacatgca aaccgctcag gtcggcatta tgttgaccat
      961 ttacgcatgg gtagtagcgc taatgtcatt gccttttatg ttaatgacca gccaggttga
     1021 acggcgcaaa ttactgatct gcctgtttgt ggtgtttatt gccagccacg tactgtcgtt
     1081 tttatcgtgg agttttaccg ttctggtgat cagtcgcatt ggtgtggctt ttgcacatgc
     1141 gattttctgg tcgattacgg cgtctctggc gatccgtatg gctccggccg ggaagcgagc
     1201 acaggcattg agtttaattg ccaccggtac tgcactggcg atggtattag gtttacctct
     1261 cgggcgcatt gtggggcagt attttggctg gcgaatgacc ttcttcgcga ttggtatcgg
     1321 ggcgtttatt acccttttgt gcctgattaa gttacttccc ttgctgccca gtgagcattc
     1381 cggttcattg aaaagcctcc cgctattgtt ccgccgcccg gcattaatga gcatttattt
     1441 gttaactgtg gtggttgtca ccgcccatta cacggcatac agctatatcg agccttttgt
     1501 gcaaaacatt gcgggattca gcgccaactt tgccacggca ttactgttat tactcggtgg
     1561 tgcgggcatt attggcagcg tgattttcgg taaactgggt aatcagtatg cgtctgcgtt
     1621 ggtgagtacg gcgattgcgt tgttgctggt gtgcctcgca ctgctgctac ctgcggcgaa
     1681 cagtgaaata cacctcgggg tgctaagtat tttctggggg atcgcgatga tgatcatcgg
     1741 gcttggtatg caggttaaag tgctggcgct ggcaccagat gccaccgacg tcgcgatggc
     1801 gctattctcc gggatattta atattggaat cggcgcgggt gcgttggtag gtaatcaggt
     1861 cagtctgcac tggtcaatgt cgatgattgg ttatgtgggc gcggtgcctg cttttgccgc
     1921 gttaatatgg tcaatcatca ttttccgccg ctggccagtg acactcgaag aacagacgca
     1981 atagttgaaa ggcccattcg ggcctttttt agtggtacgt tttaatgatt tccaggatgc
     2041 cgttaataat aaactgcacc cccatacata ccagcaggaa tcccatcaga cgggagatcg
     2101 cttcaatgcc ccccttaccc accagccgca taattgcgcc agagctgcgt agacttcccc
     2161 acaaaataac cgccaccagg aaaaagatca gcggcggcgc aaccatcagt acccaatcag
     2221 caaaggttga actctgacgc actgtggacg ccgagctaat aatcatcgct atggttcccg
     2281 gaccggcggt acttggcatt gccagtggta caaaagcaat attggcgctg ggttcatctt
     2341 ccagctcttc cgacttgctt ttcgcttccg gtgaatcaat tgctttctgt tgcggaaaga
     2401 gcatccgaaa accgataaac gcgacgatta agccgcctgc aattcgcaga ccgggaatcg
     2461 aaatgccaaa tgtatccatc accagttgcc cggcgtaata cgccaccatc atgatggcaa
     2521 agacgtacac cgaggccatc aacgactgac gattacgttc ggcactgttc atgttgcctg
     2581 ccaggccaag aaataacgcg acagttgtta atgggttagc taacggtagt aacaccacca
     2641 gccccaggcc aattgcttta aataaatcta acattggtgg ttgttatcct gtgtatctgg
     2701 gttatcagcg aaaagtataa ggggtaaaca acgataaagt gtcactcttt agctagcctt
     2761 gcatcgcact gaccaaaact tgaaccgatt tagcaaaacg tggcatcggt caattcattc
     2821 atttgactta tacttgcctg ggcaatatta tcccctgcaa ctaattactt gccagggcaa
     2881 ctaatgtgaa aagtaccagc gatctgttca atgaaattat tccattgggt cgcttaatcc
     2941 atatggttaa tcagaagaaa gatcgcctgc ttaacgagta tctgtctccg ctggatatta
     3001 ccgcggcaca gtttaaggtg ctctgctcta tccgctgcgc ggcgtgtatt actccggttg
     3061 aactgaaaag agtgttatcg gtcgacctgg gggcgctgac ccgtatgctg gatcgcctgg
     3121 tctgtaaagg ctgggtggaa aggttgccga acccgaatga caagcgcggc gtactggtaa
     3181 aacttaccac cagcggcgcg gcaatatgtg aacaatgcca tcaattagtt ggccaggacc
     3241 tgcatcaaga attaacaaaa aacctgacgg cggacgaagt ggcaacactt gagcatttgc
     3301 ttaagaaagt cctgccgtaa acaaaaaaag aggtatgacg atgtccagac gcaatactga
     3361 cgctattacc attcatagca ttttggactg gatcgaggac aacctggaat cgccactgtc
     3421 actggagaaa gtgtcagagc gttcgggtta ctctaaatgg cacctgcaac ggatgtttaa
     3481 aaaagaaacc ggtcattcat taggccaata catccgcagc cgtaagatga cggaaatcgc
     3541 gcaaaagctg aaggaaagta acgagccgat actctatctg gcagaacgat atggcttcga
     3601 gtcgcaacaa actctgaccc gaaccttcaa aaattacttt gatgttccgc cacataaata
     3661 ccggatgacc aatatgcagg gcgaatcgcg ctttttacat ccattaaatc attacaacag
     3721 ctagttgata acgtgacaac gtcactgagg caatcatgaa accactttta tccgcaatag
     3781 cagctgcgtt tattctcttt tccacgcagg gcattgcgga acaaaccacg cagccggttg
     3841 ttacttcctg tgccaatgtc gtggttgttc ccccatcgca ggaacaacca ccgtttgatt
     3901 taaatcacat gggtactggc agtgataagt cggatgcgct cggcgtgccc tattataatc
     3961 aacacgctat gtagttattg tcaggctctg atcacagagc ctgatatctc cgtaatatct
     4021 tcctgaagtc tcaaatattt tcgccattgt tggcgatgag atccctatac cagtaaaacg
     4081 acgctttacg tatccgttcc agggttccct tcccttcgtt gtctttatcg acgtaaatca
     4141 ttccgtagcg ctttttcatt tcccctgttc cggcagaaac caggtcaata cagccccatg
     4201 gggtgtagcc aattaagtca acaccatctt cgactacggc ttttttcatt tcccgaatat
     4261 gggaagcaaa gtaatcaatg cgatagtgat cgttcaccgt gccgtcagct tgtctctggt
     4321 caaccgcacc aaatccattt tcgacaataa acagcggcaa ctggaaatga tcccagaacc
     4381 agttgagtga ataacgtagc cctgccggat caatttgcca tccccattcg gatgtatcga
     4441 tataagggtt gctgaccaaa tctcgtggtt caacataatt cagttgcggg ttatctggcg
     4501 aaaattgcgt agtaaaagac atgtagtagc taaagccgat aaagtcgaca caacctctgg
     4561 caagaatcgc attatcttct ggtgtgatat cgaggttgaa tcctttcctg gcaaagtaat
     4621 tcagcatatg ttgcggataa tatccacgcg catgaacatc agtaaaccag taacgacgat
     4681 gcatcgcttt cgtggccatc atcatatcgt tgggtgcaca agtcagagga tagatggggc
     4741 acatggcgat catacagccg atattaaatt caggattgat cgatttacca gtctgtactg
     4801 ccagggcact ggcaactaac tcgtaatgta ccgcctggta cattatttgt tcgcgctcat
     4861 tgatatcttc ctctggcgaa tacaagatac cggaattagt aaatggacac aggctttcgc
     4921 tgaaattcac ctgattattg atttcgttaa acgtcatcca gtactttact ttttctttat
     4981 agcgcgtgaa gaccgttgat gcgaagcgga tgaaaaagtc gatcagttta cggtttcgcc
     5041 agccaccata ttttgtcacc agatgataag gcatctcaaa atgcgaaagc gtcaccacag
     5101 gttccattcc ctgcttcagg cattcatcga acagatcatc ataaaatttt aaaccctctt
     5161 cattcggctc ctgttcgtca ccttgcggaa agattcgtgt ccaggcaatg gaagttcgaa
     5221 agcatttgaa tcccatctcg gcaaataact gaatatctgt tttatagcga tgataaaaat
     5281 caattgcttc atgattggga taattaagtc cgtcgataac gccttctgtc acttcacgcg
     5341 gcacaccgtg agcgccagca gtcatcacat cagcgatact gatgcctttt cctccttcat
     5401 tccagccacc ttccaactga tgcgcggcta ccgcgccgcc ccataaaaaa ccttttttaa
     5461 atcctgacat acacaactcc ttaactaaaa tagattacca ccagacttcc gcctggatgc
     5521 cggccataaa ctcgtctttg ctgttctcat taaacttaaa attcgataat tcgttatcca
     5581 gaatattgat atacgttcca tagaagcgaa tttctggtcg tgaacctaaa atgctttcac
     5641 ccactttcaa tgcatgccag agtgtcgttt tataagccga ttcatttagt gttacaccct
     5701 gctgtgtttt gttctgttgc ttaaaccagc ctaattcaag ccccgtctga ttccatgtat
     5761 tccagatcca ggccggtctt actacggtgc gaatactgtc aaaatcacta tgagcgccac
     5821 tttcataact ataaacatct tcgccatgag aatagacaag cgcgttagcc ataataatat
     5881 tgtcagtaag atacatctcg ccctgagaga ttaaacgcca ggcgatccca ttggtatggt
     5941 caccatagta atagcgacca tgcgccatcg tgttactggc atctgagaaa ctggcaaaac
     6001 tgctggcata ggaattattg gcaacctgta acgtaaattc attaaacgta tcgcgttgca
     6061 ggttttgccg taaaacagaa gtcagcatcc agctatcttt catttcaaaa taactgtcgt
     6121 cattttcatt atcttgttgt tgatccgttt tatttggtgc ggaatattta gccattaatg
     6181 ataatgttgc atcatcccat aacgggatat tgcgataacg aacatccaca gaattggtat
     6241 tcatctgact ggtacgcgta aaatcacgag agtaaacatc gacatcatca cggcttaagg
     6301 acatatcaaa cagccctaca ccaagtgccc agttttcaat ccccacaccc gcagcgacat
     6361 ccgtggttaa ggttttccag tccagcattt ggatctcata ttgcgggagt ttatgtttgc
     6421 ctacccagaa gtctgcctct ggcgcgaagg gtaaaaaacc tcgcgttgtc agataaatat
     6481 cactgaactg catgatattt tcattggcac tgtcaccaaa ccaggcatcg ttatactgct
     6541 cacctacgtt gccatcatag gtaacgaccg catttgccgt tttaccgtcc tgattataaa
     6601 cacgctgatt taaggttaag tcaaaccaac cgctcatctc gttaccaaaa cgtcccagag
     6661 aacccgctgc ataagtttgt ggtgagccat gatttccggt accccagcct gagcggaagt
     6721 acccctgata gctgaaccca atatcatcct ttatatattt actgatatct ttcagggtca
     6781 cgctttcgat agtggttttg tcgcctttac tttcaataac ggcaacttgc tgcgatccat
     6841 ttatagtatg cgctgcggca gacgagttct gttcaccgtt ttcatttttg atgttatcag
     6901 cgacgggaga tgtcgctgat atttcagcaa gtgaggctga tttgtattta ttttccgtaa
     6961 tacttttttg taaggtcgaa agtcgggatt tatatacccc cagttcattc tgtgttgctc
     7021 tcagctcttg tttattttgc gacaattcat tttcaagcag ttcaaggcgc tgctcaatgg
     7081 ttaattttgt tgccaacgcg ttcaacggaa acaatgctgt aataatagaa aaagacaaaa
     7141 ggctgacgtt aatcgtcttt atattcatga cttatcctta agagtgtaat aatttcctcc
     7201 agctatcgca ctggaggaaa aaatcaggca tggagctctt ttcttaatga aataatttcc
     7261 ctggctaaat cctggcataa tattgcattc atcaaatgat cctgtgcatg aaccagaatt
     7321 aaattaactt ctattttgcc acaaccttca tcctgactaa ttaacgcggt ctgaatctta
     7381 tgtgcctgaa gacaggctaa ttccgaactg gccattagct gagttgcgcc ctgccagtct
     7441 ttttgcccgg cacaacgtat ggcttccatc gcatcagaac gtgcctgtcc cgcgttgatc
     7501 agtaactcca taacaagttc ttcatctgca aacatcaaca tctccttcct gtgtaaatgg
     7561 gattatccgt tagttgtcat gttattgacc ttgttggcgg caataacaaa aggtaaatag
     7621 atcattgtcg ccacacctaa acataaaaaa cccaccagta acgccaacca gtttcccccg
     7681 gttccgagga aagcaattaa aggtccgggc gtagtccagg gcacggcata agcaaccggt
     7741 ggaataattt ccatcgaaac aaagaagtaa ccaatggcac agttaacaat gggaaccaga
     7801 ataaacggca ccatcatgat ggggttaagt acaataggaa ggccgaacat tatcggttca
     7861 ttgatattga aaataccggg gataaacgac atttttgcca gatcacggta ttccgcacgc
     7921 ttagacgcga taaaaatagc caacaataac cccaacgtca tacctgaacc gccgcagttg
     7981 gcgaatgcat cattaataga agtccaggta atcggatatg gcgcgcccca ggtagtgccg
     8041 tgttgagcgg cccaggagag attctccaga ttagcctcag aaaaaacagt ttctcgaatt
     8101 gccgaggtgg tattaggtcc gtggatcccc agcacccaca ggaaattacc cacagccccc
     8161 aggataatta ccgcaaagat attcgttccc atatgtttga gcggcgtctg gattaatgtg
     8221 taaatgagat cgtttaatcc tgccggggat atcagtgtca ggcaataatt aagtgcggaa
     8281 aagaacacca tgacaaaaaa tattggcaat aaaactttaa atgaacgcga taccgcagga
     8341 ggtacagctg ccggcatcgt tatggtgatt tttgggtttc gagctaagcg acaaaatatt
     8401 tcactggtga ttaatgcaac gataacagca acaaacaccc cctgtgcgcc gagatatttg
     8461 gtcgtcagga atgcttgccc atctatcatc tgatatggcg tataaacaat aaaaaatgct
     8521 cctattgccg ttaatccgca taaaagatca tcttgctcat agctaatcgc catatttctg
     8581 gcgaccagga aagcaatcat aattgacatg atatttacag agccattcat taccggagta
     8641 aaaatagctt gtgctttttc aaggttgggg aaaacgcggt tcagatgcag cagcccggca
     8701 ataaagccgt cgggcgataa tatggcaaag ttaattaata taattaatga gcttgccatg
     8761 ataattggaa acgtaaaaac gaatccatct ctcaccgctg caacatgttt ttgtgagttt
     8821 aacttgatag caactggaac aagaaaacgt tccattccac gttcgaatga ggtcattaat
     8881 cccatttttt atacctgttg ttctggatat tta
//
LOCUS       DACBVI010000167         8903 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.168, whole genome shotgun sequence.
ACCESSION   DACBVI010000167 DACBVI010000000
VERSION     DACBVI010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.168"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..2141
                     /locus_tag="IU325_19505"
     CDS             <1..2141
                     /locus_tag="IU325_19505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HAP5811191.1"
                     /translation="TAQTNEHQTLSELFYKELEDKFSGKELATPLLKSFSENCRQNGR
                     HIFSNKDFVIKFSTSVLQTDKKEITIINKNENTTLTQTIAPIFEEYLMEILPQRSDAL
                     DKQKLNLKSDRKEKEFPRIKLNGQCYFPGRPQNRIVCRHIAAQYINDIYQNVDYKPHQ
                     DDYSSAEKFLTHFNKKCKNQTLALISSRPEGRCVAACGDFGLVMKAYFDKMESNGISV
                     MAAILLVDNHALTVRLRIKNTTEGCTHYVVSVYDPNVTNDKIRIMSESKEDIKHYSLM
                     DFMNVDYSLLKWSNDHVINQSVAIIPALPKEQLLMLKGSVDEIPPPLSPATMNLLMAI
                     GQNHQLTQLMTQLQKMPELHRTEMLTAYNSINLPGLYLAINYGNADIVETIFNSLSEP
                     GYEGLLSKKNLMHILEAKDKNGFSGLFLAISRKDKNVVTSILNVLPKLAATHHLDNEQ
                     VYKFLSAKNRTSSHVLYHVMANGDADMLKIVLDALPLLIRTCHLTKEQVLDLLKAKDF
                     YGCPGLYLAMQNGHSDIVKVILEALPCLAQEINISASDIVDLLTAKSLARDTGLFMAM
                     QRGHMNVIKTIFNALPTLFNTFKFDKKNMKPLLLANNSNEYPGLFSAIQHKQQNVVET
                     VYLALSDHARLFGFTAEDIMDFWQHKAPQKYSAFELAFELDHRVIAELILNTINKMAE
                     SFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(2110..3846)
                     /gene="aceK"
                     /locus_tag="IU325_19510"
     CDS             complement(2110..3846)
                     /gene="aceK"
                     /locus_tag="IU325_19510"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HAP5811192.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIVRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCAAPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3895..5199)
                     /gene="aceA"
                     /locus_tag="IU325_19515"
     CDS             complement(3895..5199)
                     /gene="aceA"
                     /locus_tag="IU325_19515"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HAP5811193.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(5229..6830)
                     /gene="aceB"
                     /locus_tag="IU325_19520"
     CDS             complement(5229..6830)
                     /gene="aceB"
                     /locus_tag="IU325_19520"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HAP5811194.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMCANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(7099..8028)
                     /gene="metA"
                     /locus_tag="IU325_19525"
     CDS             complement(7099..8028)
                     /gene="metA"
                     /locus_tag="IU325_19525"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HAP5811195.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEFFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            8185..8628
                     /locus_tag="IU325_19530"
     CDS             8185..8628
                     /locus_tag="IU325_19530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP5811196.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRVELEELVR
                     SFLPEAPLWVAVNERDRPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNKQAVGFYKKMGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2308 a         2167 c         2164 g         2264 t
ORIGIN      
        1 atacagccca gacaaatgaa caccaaaccc tgagtgaatt gttttataaa gaactcgagg
       61 ataaattttc tggcaaggag ctggcgacgc ctctattaaa aagcttctca gagaactgta
      121 gacaaaatgg tcgtcatatc tttagtaaca aggattttgt cattaagttt tccacgtccg
      181 tcttacagac tgataaaaaa gaaattacga taattaataa aaatgaaaac acgacactca
      241 ctcaaaccat tgctccaata tttgaagaat acctgatgga aattttacct caacgctcag
      301 acgctcttga taaacaaaaa ttaaatctaa aatcagatag aaaagaaaaa gaatttccaa
      361 gaattaaact taatggtcaa tgttattttc cggggcgacc ccaaaaccgt atagtatgcc
      421 gacacattgc tgcacaatat attaatgata tttatcagaa tgttgattac aaaccccatc
      481 aagatgatta ctcttcagct gaaaaatttc tcactcactt caacaaaaaa tgcaaaaacc
      541 agactttggc gttgatttcc agccgtcctg aggggcgttg cgttgctgcc tgcggtgatt
      601 tcgggctagt tatgaaagca tattttgaca agatggaatc aaatggcatc agtgttatgg
      661 cagccatatt attggtggat aaccatgctt tgacggtccg gctaagaata aagaacacaa
      721 ctgaaggatg tacccattac gtggtttcgg tttatgatcc aaatgtaact aacgataaaa
      781 taagaatcat gagcgaaagt aaagaggata ttaaacacta ttcactgatg gactttatga
      841 atgtagatta tagcctcctg aaatggtcaa atgatcatgt tattaaccaa tctgttgcaa
      901 taattccagc acttccgaaa gaacagctat tgatgctaaa aggatctgtg gatgaaatac
      961 cccctccatt atcacctgca acgatgaatt tgctaatggc aattggtcag aatcatcaac
     1021 ttacgcaact gatgactcaa ctccagaaaa tgccagaact acatagaaca gaaatgttga
     1081 ctgcctataa tagtattaac ctccccggtt tatatttggc tataaattat ggtaatgcgg
     1141 atatcgttga gactattttc aattcattgt cagagccagg atatgaaggg ttactctcga
     1201 aaaaaaatct catgcatatt ctggaggcaa aagataaaaa tggtttttct ggattatttt
     1261 tagcgatatc acgtaaggat aaaaatgttg taacctcgat tctgaacgtc ttacctaaac
     1321 tggccgcaac acatcattta gataatgaac aggtgtataa attcctgagt gccaaaaata
     1381 gaacgtccag ccatgtttta taccatgtta tggcgaatgg tgatgccgac atgctgaaaa
     1441 ttgttttgga cgcgttacct ttgttaattc gcacatgtca tttgactaaa gaacaggtac
     1501 tcgatctcct gaaggcaaag gatttttatg gttgcccagg actatacctg gcgatgcaaa
     1561 atggacatag cgatatcgtg aaagttattc tcgaagcatt accctgtctg gcccaggaaa
     1621 ttaacatttc agcttccgat attgtagatc ttctgaccgc taaaagtctt gcgcgcgaca
     1681 cgggtttgtt tatggccatg cagcgcggac atatgaacgt tattaagact atttttaacg
     1741 cattacccac tctgtttaat acgtttaaat tcgataaaaa aaatatgaag cccctcctcc
     1801 tggcaaataa ttctaatgaa tatcccggtt tgttttcagc gatacagcat aaacaacaaa
     1861 acgttgtaga gacggtttat cttgctttat ctgaccatgc acgcctgttt ggatttactg
     1921 ctgaagatat tatggatttt tggcaacata aagcgccaca aaaatactct gcctttgagt
     1981 tggcttttga attggatcac cgggttattg ctgaattaat ccttaatacg ataaataaga
     2041 tggctgaaag ctttggcttt acggataacc ctcgatacat tgcggagaaa aattatatgg
     2101 aagctttact caaaaaagca tctccccata ccgtacgcta aatctttgcc tgcgccgata
     2161 cgcataaaca tcttccacat gtccttcacg aatacggttt tgtagtgcgc gccagtaatc
     2221 agcgcggaac aggtcggcgt gcatctcttc aaagagtggc ccaatgcggg gggcggcgca
     2281 tagccagtgg cgaaactctt ccgggaaaac atcgcccggc gagacgctgt accacggttc
     2341 gctggcaagt tcgtcctccg ggtagcgcgg cggcgggatg tcgcggaagt tcacttccgt
     2401 catgtagcaa atttcgtcgt aatcataaaa caccacacgc ccgtgacggg tgacaccgaa
     2461 gtttttaaac agcatgtcgc cagggaaaat gttagcagcg gcaagctggc gaatagcgtt
     2521 accgtattct tcaatggcat cgcgcaactg ctgaccttcc acttgttcca gccagatatt
     2581 gagcggcacc atccgccgct caatataaag atggcgaatc acaatttgtt cgccgagatc
     2641 ggtgattttt tccgctgctt cctggagcag taattccatt aatgccgggg aaatatgccg
     2701 cttctccagc acaaagtttt caaactcctg ggtgtccgcc attcggccca cgcgatcgtg
     2761 ctctttcacc aattgatagc aggcacgaac gtgagcggca gacatctctt tctgcggcgc
     2821 gaacttgtct ttgatgactt tgaatacccg atcaaagccc ggcagcgtaa acaccagcat
     2881 caccatgcca cgaatacccg gcgcttcaat gaactgctca ttacagccct gtagataaac
     2941 gagatattcg cggtagcttt cggttttggc gtgcttctgg cagccgatag ccatatacaa
     3001 ttcagcggtg gttttacctg gcagaatttc ccgtagccac tcgaccagcg ctgcgggcag
     3061 cggtgcataa accataaaat aagaacgcgc aaagccaaag acaatgctcg cttcggcggt
     3121 cgtcgtcaga caggtatcaa taaacagttc gccgtcgtcc gtctggtgga tcggcagcaa
     3181 aaatggcaat gtgccggaag gcgtgatcag tttgcccacc agccaggcgg ctttattgcg
     3241 gtaaaacagt tcgttcgcca cctgtaaatg actttccgcg aggttgtctg tccccagcgt
     3301 ttccgtcaga tgacgaacga tgtaatgaat gtcacggctt ttattctgcc agcgcagacg
     3361 caacggcagg tcgctgataa cgcgcatcag cagagattcc cagccgtgat cggggtgaaa
     3421 gtctttcgcc agcgggcgtg gaatggtacg aaagcggcgc tctggctggg agctaaagat
     3481 aaaaagccgc tcgggagtaa gcgagcggtg gtcaaataac cgacagtaca cggagttaaa
     3541 aaaactctcc gcaatctcga agcgcgggta atccggcaac agccgggtgt aatgctcttt
     3601 gacgcgcagt aaaaatgccg cgtccgtgct ttggccgttg gtaatgcagc gcagttgctc
     3661 caccaccaga cccacgtgat gatcgtaaag atggatacgg tttttcatcg cctgctggac
     3721 ggcgtgccag tcggcttgtt cgaaacgctg ctgcgccccg gaagtcactt cgaggaatcg
     3781 accatactga gcgtcgaaac cttgcaaaat ggtttgtgca atcagtaatt ccaggccacg
     3841 cggcatcgcc tctccttaat tcgcccctcg ccttacggaa aggggcgttg gttgttagaa
     3901 ctgcgattct tcggtggagc cggtcagcgc ggtgactgaa gacgtgccgc cctgaataat
     3961 ggtcgtcact ttatcgaaat aacctgtacc cacttcctgc tggtgagata cgaaggtata
     4021 gccatctttc gcagcggcaa attccggctg ctgcactttc tcaacgtaat gcttcatgcc
     4081 ttcgccctgg gcataggcat ttgccaggtc gaacatgtta aaccacatgc tgtggatgcc
     4141 tgccagggtg atgaactgga acttgtagcc catatccgac agctgctgct ggaagctggc
     4201 aatagttttg tcgtcgaggt ttttctgcca gttgaacgac ggcgagcagt tataagccag
     4261 cagtttgccc ggatatttcg cgtggatggc ttgtgcaaag cgacgcgcca gttccagatc
     4321 cggcgtggaa gtttcacacc ataccaggtc agcatatggc gcatacgcca ggccacggct
     4381 gatcgcttgc tcaatgcccg catgagtacg gaagaaacct tcgctggtac gctcgccggt
     4441 aataaattcg ctgtcatatg ggtcgcaatc ggaggtaatc agatccgccg catcggcatc
     4501 ggtacgagca accagcaggg ttggaacgcc tgtcacgtca gctgccagac gcgccgcgac
     4561 cagtttctga atagcttcct gagttggcac taaaactttg ccgcccatgt gaccgcattt
     4621 cttcactgat gccagctgat cttcgaagtg aactgccgct gcaccggctt caatcatcgc
     4681 tttcatcagt tcaaaggcat tcaggacacc gccaaaaccg gcttccgcat cagcaacgat
     4741 cggcaggaag taatcgacat agcgcggatc gcccggctca atgcccgcgg accattggat
     4801 ctgatccgca cggcggaaag tattgttgat ccgctccacc acagctggca ccgagtttgc
     4861 cggatagagc gactgatccg gatacatgct ggccgccagg ttagcgtccg ccgctacctg
     4921 ccatcccgac agatagactg cttcaatacc cgctttcgcc tgttgcagcg cctgaccgcc
     4981 agtcagtgca ccgaggctgt tgatgtagcc ttttttcgac tcgccgtgca gcagacgcca
     5041 cattttcgcc gcgcccagtt gcgccagcgt gcattcagga ttgactgaac cgcgtaattt
     5101 caccacatct tccgcgctgt atgggcgagt aatgccttcc caacgcggtt gagtccactc
     5161 tttctgtaat tcttcaattt gttgtgtacg ggttttcatg tgcagatgct ccatagttat
     5221 gtggtggttt acgctaacag gcggtagcct ggcagggtca ggaaatctat taactcatcg
     5281 gaagtggtga tctgttccat caagcgtgcg gcatcgtcaa aacgcccctg ggagaaacgt
     5341 tcttcgccca gttcgctggc aatgactttc atctcttcgc ccagcatctg gcggaacaag
     5401 gctttggtca ccggtttgcc attgctcaac gttttttgat gatggatcca ctgccagatc
     5461 gaggtacggg aaatttcagc cgtcgccgca tcttccatca ggccataaat cggcacacag
     5521 ccgttgccag agatccatgc ttcgatgtac tgcacagcca cgcgaatgtt ggcgcacata
     5581 ccttcttcgg tgcgttcacc atcacaaggt gccagcagct gatcggcagt aatcggcgcg
     5641 tcttgttcgc gcatcacttc aagctgattt ttacgggagc cgagaatgtc gttgaatacc
     5701 gccattgccg tatccgcaag gcctgggtgg gcaatccatg tgccatcgtg accgttatta
     5761 gcttccagcg atttatccgc ttttactttg ttgagcacct ggttattgcg ctcttcatct
     5821 ttgctcggaa taaatgccgc cataccgccc atcgcaaaag caccgcgttt atggcaggtt
     5881 ttaatcaaca ggcgtgaata agcattcagg aatggtttat ccatcgtcac tgcctgtctg
     5941 tctggcagga cgcgatcggg atagtttttc aacgttttga tataactgaa gatgtaatcc
     6001 caacgaccgc agttcagacc aacaatatgg tcacgcagcg cgtgaaggat ttcgtccatc
     6061 tggaacacgg cgggcagcgt ttcaatcagc aacgtcgcct tgatggtgcc gcgcggcaga
     6121 ttaaagcgat cttctgcata gctgaagact tcgctccacc aggccgcttc ctgccaggac
     6181 tgggttttcg gcagatagaa atagggaccg ctgccctttg ccaacagtgc ctgatagttg
     6241 tggaagaaat agagcgcaaa atcaaacagg ctaccgggga ttgcctcacc acgccaggtg
     6301 acatgttttt ccggcaagtg cagaccgcgt acccgacaaa tcaacaccgc tggattgggc
     6361 ttgagctgat aaattttgcc tgcttcattg gtatagctga tggtgccgtt aactgcatca
     6421 cgcaggttaa tttgcccgtc gatcactttg ttccagtctg gtgccagtga atcttcgaaa
     6481 tcggccataa agactttcac attggcgttg agcgcgttga tcaccatctt gcgctcaacc
     6541 gggccggtaa tctctacccg acgatcttgt aagtccgcgg gaataccacg aattttccag
     6601 tcagcatcac gaatggaagc tgtttccgaa ataaaatcag gcaacgttcc gttatcgata
     6661 tcctgctgct gctgaatgcg cgctgccaga agtttattgc gttgtggcgt aaaatgcgtc
     6721 accagctcag tcagaaattc taccgcttcg gcagtaagaa tttgcttctc ctgctcgcca
     6781 tacggcctta tgaaagccag ttcatcggtt gttgttgcct gttcagtcat cgtgcagctc
     6841 ctcgtcatgg atccgaaaac ttccccactg tgaacgaagg atcgttgtgc gcttttcgtt
     6901 cagcacaact aagactactc atttaaaatg caaaatcaaa aacaatttcc attttaatta
     6961 aaaatacttg ataacttatt gataacaata tgaataaaat ttattcacct actgaggtgc
     7021 gtttcggaaa taaaaaaggc acccgaaggt gcctgaggta aggtgctgaa tcgcttaacg
     7081 atcgactatc acagaagatt aatccagcgt tggattcatg tgccgtagat cgtatggcgt
     7141 gatctggtag acgtaatagt tgagccagtt ggtaaacagc aaattaccgt gactacgcca
     7201 gctcgctcgc ggtgtatttt gcggatcatt atgcggaaaa tagttatacg gtacatccgg
     7261 gtccagtccg gcttccacat cgcggaaaaa ttcctgcgcc agcgtttgcg catcatattc
     7321 gggatggccc gtcacaaagg caatgcgctt atctttgctg gcaaacagat atgcatcccc
     7381 ttcttccgtc tctgccagaa tttccagatc ggtataatca cgaatcaacg ctgccggaaa
     7441 gtcagcatag cgcgaatgtg gtgccaggaa tgaatcatca aagccacgcg tcagaagcgc
     7501 atgaggatgg agaatatgat gctcgtaaac gccagagagt ttgtcggtgc gggtttgctt
     7561 aggaatgccg tagaggatat tgagcgcggc ctggaccgcc caacagacaa acagcgtcga
     7621 ggtgacgtga tctttcgacc actccagcac ctgtttgatc tgcggccagt aagcgacatc
     7681 attaaactcc accaggccca gcggcgcgcc ggtaactatc agaccgtcaa agttttgttc
     7741 ctgaatatct tcaaagttac agtagaagtt attcagatgc tctgcgggcg tgttgcgcga
     7801 ttcacgggaa tcgatgcgca acagctgaat atcgacctgc aaaggtgagt ttgaaagcag
     7861 gcgcagaaac tgattttcag tttcaatctt cttcggcatc aggtttagga tcagaacctt
     7921 aagtggacga atttcctgac cagacgcacg agaagttgtc atcacaaaaa cgttttcttc
     7981 acgcaagaaa ttgacggcgg gtagctcgtc cggcacacga atcggcataa cctgattacc
     8041 tcactacata cgcttatacg tttagacatc cagatagctg aagataacca gaaaatttgc
     8101 caatgtcgag cctgcatgtt gaaggtgaga aagtttcaag gaaaaggcta cgttagaata
     8161 taagaatgac gaaaaggaga gaaaatggtt attagtattc gccgttcgcg gcatgaggaa
     8221 ggggaggaac tcgttgcgat ttggtgtcga tctgtcgatg ccacccacga ttttttatca
     8281 gcagagtatc gggtcgagct ggaagagttg gttcgttcct ttctgccgga agcaccgttg
     8341 tgggtcgcgg ttaatgagcg ggatcggccg gttgggttta tgttgttaag tgggcagcat
     8401 atggatgcgc tgtttatcga tcctgatgtg cgcggctgcg gcgtgggtcg gatgctggtg
     8461 gaacatgcgc tctcgatggc accggaactg acaaccaatg ttaatgagca aaataagcag
     8521 gctgttgggt tctataagaa gatgggtttt aaggttacgg gacgctctga ggtggacgat
     8581 ttggggaaac cgtatccgtt gctgaatctg gcgtatgtgg gggaataagc tgcccctgcc
     8641 gtgcctgccc aggctggaga agacgcgcca gcgtcgcatc cggcattttt tttcaccagc
     8701 gcaaaaaggc catccgtcag gatggccttc tgcttaattt gatgcctggc agtttatggc
     8761 gggcgtcctg cccgccaccc tccgggccgt tgcttcgcaa cgttcaaatc cgctcccggc
     8821 ggatttgtcc tggttgggag agcgttcacc gacaaacaac agataaaaca aaaggcccag
     8881 tctttcgact gagcctttcg ttt
//
LOCUS       DACBVI010000168         8763 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.169, whole genome shotgun sequence.
ACCESSION   DACBVI010000168 DACBVI010000000
VERSION     DACBVI010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.169"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..167
                     /gene="ralR"
                     /locus_tag="IU325_19535"
     CDS             <1..167
                     /gene="ralR"
                     /locus_tag="IU325_19535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HAP5811197.1"
                     /translation="FCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRT
                     TGNNNGGVHV"
     gene            160..369
                     /gene="rcbA"
                     /locus_tag="IU325_19540"
     CDS             160..369
                     /gene="rcbA"
                     /locus_tag="IU325_19540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break reduction protein RcbA"
                     /protein_id="HAP5811198.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLQSSRIALYSN"
     gene            448..663
                     /gene="xisR"
                     /locus_tag="IU325_19545"
     CDS             448..663
                     /gene="xisR"
                     /locus_tag="IU325_19545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HAP5811199.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            665..1900
                     /locus_tag="IU325_19550"
     CDS             665..1900
                     /locus_tag="IU325_19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HAP5811200.1"
                     /translation="MSKLPTGVEIRGRYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEINSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTLISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNMWKFAVYSGLRHGELAALAWEDVDLTKGIVNVRRNLTILDMFGPPKTNAGIRTVTL
                     LQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNGKQKPYYSVS
                     SLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQIAMLNARLS"
     gene            1952..2887
                     /gene="ttcA"
                     /locus_tag="IU325_19555"
     CDS             1952..2887
                     /gene="ttcA"
                     /locus_tag="IU325_19555"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HAP5811201.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(3016..4389)
                     /gene="dbpA"
                     /locus_tag="IU325_19560"
     CDS             complement(3016..4389)
                     /gene="dbpA"
                     /locus_tag="IU325_19560"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HAP5811202.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPVNSSIVPLETEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGRIKGKT
                     CRVRLLK"
     gene            complement(4867..5850)
                     /gene="zntB"
                     /locus_tag="IU325_19565"
     CDS             complement(4867..5850)
                     /gene="zntB"
                     /locus_tag="IU325_19565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HAP5811203.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            6105..7337
                     /gene="dgcM"
                     /locus_tag="IU325_19570"
     CDS             6105..7337
                     /gene="dgcM"
                     /locus_tag="IU325_19570"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HAP5811204.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRNEEETALSCRLVLRKLTEAEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCTLARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(7358..7921)
                     /gene="smrA"
                     /locus_tag="IU325_19575"
     CDS             complement(7358..7921)
                     /gene="smrA"
                     /locus_tag="IU325_19575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HAP5811205.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(8116..>8763)
                     /locus_tag="IU325_19580"
     CDS             complement(8116..>8763)
                     /locus_tag="IU325_19580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5811206.1"
                     /translation="LGQVRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLM
                     AGLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKL
                     ASGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFD
                     RSGSDQTSEILASTKLLSQLMLREAQTMAFSDTFLLISGLLFIAFLLVPAMNKSS"
BASE COUNT         2490 a         2128 c         2032 g         2113 t
ORIGIN      
        1 cattttgtgg ttgtccatca gtaacggtga aagccatttc aggatattac cgagcgaagt
       61 gtaacggatg cgaatcccga accggttatg gtggaagtga aaaagaagca ctcgaaagat
      121 ggaataaacg aaccactgga aataataatg gaggtgttca tgtataaaat taccgccact
      181 attgaaaagg aaggtggcac tcctactaac tggacaagat attcaaaatc taaactaacg
      241 aaatcagaat gcgaaaaaat gctctcaggt aaaaaagaag caggcgtttc cagagagcag
      301 aaagtaaaac tgataaattt taattgcgag aaacttcagt cctcgagaat tgcattgtat
      361 tcaaattaaa acttcatagc tgattattaa taatcaacat cgggcgtcaa tttcagtcta
      421 acattggcgc ctgccagagg tgatgcgatg gcacaagtaa tctttaatga agagtggatg
      481 gttgaatacg gcctgatgct tcgcactggt ctgggggcca gacaaattga agcataccgc
      541 cagaactgtt gggtggaggg cttccacttc aaacgagtat ctcctttagg taagccagac
      601 agcaaacgag ggattatctg gtacaactat ccaaagataa atcagtttat caaagactca
      661 tgatatgtct aaattaccaa caggtgtcga gattagaggt agatacattc gcatctggtt
      721 catgtttcga ggaaaacgat gtcgggaaac attaaaaggc tgggagatta caaacagtaa
      781 tattaaaaag gccggaaatt taagagcact gatagttcat gaaataaact ccggtgaatt
      841 tgagtattta agacgttttc cccagtccag cactggggca aaaatggtga caacgagagt
      901 cataaaaacg ttcggggagc tttgtgatat ctggacaaaa attaaagaga cagagttaac
      961 aacaaacaca atgaagaaaa cgaaatcaca attaaaaaca ctcagaataa taatttgtga
     1021 aagtaccctg atatcacata ttcgttatag cgatatctta aactaccgga atgaactgct
     1081 gcatggagaa acgctttacc tggataatcc aagatccaac aaaaaaggaa gaaccgtgcg
     1141 cacagttgat aactatatcg ccctgctctg ttcgctgtta cgttttgcgt atcagtcggg
     1201 atttatatca accaaaccat ttgaaggagt aaaaaaatta cagcgaaaca gaataaagcc
     1261 tgatccgtta tctaaaacag aattcaatgc attaatggaa agtgaaaaag gacaaagcca
     1321 gaacatgtgg aaatttgcag tttactcagg acttcgtcac ggggaactgg cagctctggc
     1381 gtgggaggac gtggatctca caaagggaat agtgaatgtc agaagaaacc tgacgatact
     1441 tgatatgttc ggtcccccaa aaacaaatgc cgggatccgg acagtaacac tactgcagcc
     1501 tgctcttgaa gcactgaagg agcaatacaa actgaccggg catcatcgca aaagcgaaat
     1561 caccttttat catcgggagt acggcagaac cgaaaagcaa aaactgcatt ttgttttcat
     1621 gcccagggtg tgtaacggaa aacaaaaacc ttattactcg gtaagcagtt tgggggcaag
     1681 gtggaatgca gcagtaaaac gtgctggtat tcgccgccgt aatccgtacc atacgcggca
     1741 tacttttgcc tgctggctgt tgacggcagg agcgaacccg gcatttatag ccagccaaat
     1801 ggggcatgaa actgcgcaga tggtgtatga aatttacggt atgtggattg atgacatgaa
     1861 cgacgaacag atagccatgt tgaatgcgcg gttatcatag ttgcaaagtt tgcccccaat
     1921 ttgccccatt tagtaccaga gaactgaaat aatgcaagaa aatcaacaaa ttacaaagaa
     1981 agaacaatac aacctgaaca aattacaaaa acgtctgcgt cgtaacgtgg gcgaagccat
     2041 tgctgacttc aatatgattg aagaaggcga tcgcatcatg gtttgcctct ccgggggtaa
     2101 agacagctat accatgctgg aaattctgcg caatttgcag caaagcgcgc caatcaattt
     2161 ttcgctggtg gctgttaacc tcgatcaaaa gcaaccgggc ttcccggaac acgttctgcc
     2221 cgagtatctt gaaaagctgg gcgttgagta caagattgtt gaagagaaca cttacggtat
     2281 cgtgaaagag aagattccgg agggcaaaac cacttgctca ctgtgttctc gtcttcgtcg
     2341 cggtatcctt tatcgtaccg caacggaact gggagcgacg aagatcgcgc tgggtcacca
     2401 tcgtgacgat atcctgcaaa cgttgttctt aaatatgttc tacggcggca agatgaaagg
     2461 tatgcctccg aaactgatga gcgatgatgg caaacatatc gtgattcgcc cgctggccta
     2521 ctgccgcgag aaagatattc agcgctttgc cgatgcaaaa gcgttcccga ttattccgtg
     2581 caacctgtgc ggttcacagc ctaacctgca acgtcaggtg attgctgaca tgttgcgtga
     2641 ctgggataaa cgttatccgg ggcgtatcga gacgatgttc agcgcgatgc agaatgtggt
     2701 gccgtcgcat ctgtgcgata ccaacctgtt cgatttcaaa ggcattaccc acggttctga
     2761 agtggttaac gggggtgatc tggcgtttga tcgcgaagag atccctctac aaccggcagg
     2821 ttggcagcct gaagaagatg aaaatcagtt ggatgagtta cggctgaatg tggttgaagt
     2881 gaaataaccc ggatagcgcc cgatgcgtaa gcgtatcggg ctactcttat ggaggccgga
     2941 taagacgcgg tcagcgacac atccggcaat gccgaagaag atgtttactc ttgcacccgg
     3001 caattcaaca tttcattatt ttaataaccg cacccggcac gtttttcctt taatcctccc
     3061 gccctgtaac tgtttccatg ctttatgagc aacagcctga cgcaccgcga catagacatg
     3121 cgccggatgc acggcgattt tgccaatatc tgcgccatca agcccgatat ctcctgtcag
     3181 cgcacctaat acatcacccg ggcgcatttt ggcttttttc ccgccatcga tacataacgt
     3241 tgccatttcg gtttccagcg gcacaataga actattaact ggcggcgttt gccagttaag
     3301 ttttatctgc aacatgtcag aaatgatatt ggcccgctgt gcttcttccg gggcacagaa
     3361 actgatcgcc agaccgctat ttcctgcacg cgctgtacga ccgatgcgat gcacatgaac
     3421 ttcaggatcc cacgccagct caaagttcac caccagctca agcgatttaa tatccagacc
     3481 tcgcgcagca acatcagtcg caaccagtac acgggcgcta ccgttagcaa aacgtaccag
     3541 ggtctgatcg cgatcacgtt gctccagatc gccgtgtaac gacaatgcac tttgccctac
     3601 ttcattcagc gcgtcgcaga cagcctggca atcttttttg gtattgcaaa acaccacgca
     3661 agaggatggc tgatgcaaac ttaataaccg ttgcaacaac ggaattttgc ctttgctgga
     3721 tgtctcataa aattgttgtt caataggtgg cagagcatct gttgagtcaa tttcaatcgc
     3781 taaaggatcg cgttgcactc gtccgctgat tgcggcgatg gcttccggcc aggttgccga
     3841 aaacagaagc gtctgtcgag atgctggcgc aaaacggatg acatcatcaa tggcgtcgct
     3901 aaatcccata tccagcatgc ggtcggcctc atccatcacc agcgtattca acgcatccag
     3961 tgataccgtg cctttttgca gatgatccaa caaacggccc ggcgttgcca cgataatatg
     4021 cggcgcatgt tgcaacgaat cacgctgcat accgaacggt tgaccaccgc acaacgtcaa
     4081 aattttggta tttggcagaa aacgcgccaa ccgacgcaat tcacctgcca cctgatccgc
     4141 cagttcacgc gtaggacaca gcactaaagc ctgggtttga aatagcgacg catcaatttg
     4201 ctgtaacaag ccgaggccaa aagccgccgt tttgccgctg ccggttttcg cctgcacgcg
     4261 aacatctttt ccggcaagga tcgccggaag cgcggcggcc tgcaccggcg tcatagttaa
     4321 ataacccaac tcattaaggt tcgtgagttg ggcgggaggc aaaacattca gggtagaaaa
     4381 agcggtcaca atctattctc gtggtcatcg acgcaaagtt agcaggcgcg tatcctcgca
     4441 gatctacgct cacgatgcga caatttaatc ggttcttcat cgggtggtgg gtcaggcatg
     4501 ggttgcggac gagggatcgg atcgggcact ggaacaggat cgccaggaat cggttcaggg
     4561 acaggaattt gcaaataaat aagtgtcgtc atatttccct ccggtcattg ggtggactct
     4621 taaagggtag acgctgataa ataacaggca aaaaaaagcc gactcatcaa agtcggcgtc
     4681 gtacgaatca attgtgctat gcagtaattc aaaaaaggaa gtaagacaat atggagcgca
     4741 acgcccatcg cttgacgttg cattcacctg caagagagat attgccctga atgggtagag
     4801 agtttattga cttcgctcaa actttgcggc gtttttgtat acagacatcc gggaaaattg
     4861 cttttgttac aaccatttac tacgatgcaa ccataaagca acaccaccaa taagaacaac
     4921 taacagaata caaaaaattg aaaatccgaa ttgccacccg ccgcctggga tcccaccaag
     4981 gttgacgcca aataacccgg tcagaaaggt actgggtaaa aagaccattg ccatcaacga
     5041 cattgtatag gtacgacgcg ctaaattttc ctgcatcacc tgagcgattt catccgccat
     5101 cacgccagtc cgtgctatac aggcgtcgat ttcgtcaagc ccgcgcccaa ggcgatcggc
     5161 aatatcctgc atacgacggc gttggtcatc gctcatccac ggcaaacgtt cactggcaag
     5221 acgagcataa acatcacgtt gcggtgccat atagcgacgc atcacaatta attgtttgcg
     5281 cagcagagcc aggaatccac gcggtggaat ttgctgatca aggagattat cttcaaggtc
     5341 gataatttta tcgtgcagct gctcgataaa ttcgctggaa tgatcggtca acgcatcaca
     5401 tacatccacc agccatcccc cgcaatcggt cggacccgtg ccctcttcca gatcgcttac
     5461 cacatcgtcc agcgccagca ctttgcgttg tcgggtcgaa acaattaacc gcccgtccat
     5521 atatacacgc atggcgacca gttgatcggg gcgttcatcg gtgctgccgt ttatacagcg
     5581 caatgtaatc agcgtgcctt caccgagacg gctgactcgg ggccgtgtgc tctcgcccgc
     5641 cagcgcatca cgtacgttat tgggaagcag cggtgttgtc gccagccatt gggcgctatc
     5701 atggtgtaca taattaaggt ggagccagca gggatgcgct tcatcaatca catctgtatt
     5761 ttccagcggt ttaacgccgc ctctaccatc cagcatccag gcaaatactg catccgggac
     5821 attaacgtcc gatcccttaa tcgcttccac agtgcctcca tcatcaacgc attattttgt
     5881 agtctagcct tctggccctg ttacgcaaca tctcatcacc ccattaccct gaaatgatta
     5941 ataaaattct gtctaaattg aatacaaaaa gcaaaatgct tttccatata caaaccgtgt
     6001 gaagtgttaa atagcgtcta tcattatcag aattatctga tcatatgacg tggctttttt
     6061 gcgatcggat agcaacaaaa attgataaaa ataacgggat ctcaatgatt acgcacaact
     6121 tcaataccct ggacttactc accagtcctg tctggatcgt ttcgcccttt gaggaacagt
     6181 taatttatgc caacagcgcg gcgcgactgt tgatgcaaga cctcacgttt agtcagctac
     6241 gaaccggtcc ctattccgtc tcctcacaaa aagaactgcc gaaatacctc tccgatctgc
     6301 aaaaccaaca cgatattatc gaaatcctca ccgttcagcg caatgaagag gaaacagcat
     6361 tgagttgtcg gcttgttttg cgaaagctga cagaagcaga accggtgatt attttcgaag
     6421 gtatcgaagc accggcaacg ctgggtttaa aagccagtcg ctcggcaaat tatcagcgca
     6481 aaaaacaagg tttttatgcg cgcttttttc tgactaactc tgcaccaatg ttgttgattg
     6541 acccctcacg agatggccaa atcgttgatg ctaacctcgc cgcgctcaat ttctatggct
     6601 ataaccatga aacgatgtgc cagaaacata cctgggaaat aaatatgctc gggcgacgcg
     6661 tcatgcctat catgcatgaa atttcgcatt tacccggtgg ccataagcct ttgaattttg
     6721 ttcataaact ggcggatggt tcgactcgtc atgtgcagac ctatgccggt ccgattgaaa
     6781 tttatggcga caagctcatg ttatgtattg tgcatgatat tactgagcaa aaacggctgg
     6841 aggagcagct ggaacatgct gcccaccatg acgcgatgac cggattactg aatcggcgac
     6901 agttttatca cattaccgag ccaggccaaa tgcagcacct cgccatcgct caggattaca
     6961 gcttattgct catcgacacc gatcgtttta aacacattaa cgatctctat gggcattcta
     7021 aaggtgatga ggtgttatgc accctcgccc gcactctcga aagttgcgct cgcaaaggcg
     7081 atttggtgtt tcgttgggga ggcgaagagt ttgtcttatt gctaccaaga accccactgg
     7141 ataccgcgct ttcgctggct gaaactatcc gcgtaagcgt ggcaaaagtg agtatttcgg
     7201 gcttaccacg ctttaccgtc agcattggtg tggcgcatca cgaaggaaat gaaagcatcg
     7261 atgaactgtt taaacgcgtt gatgatgctt tgtatcgggc gaaaaatgat ggacgcaacc
     7321 gcgtgctggc ggcataagcc gcggatgcgt ctcgagatca acgactgcgc ttagcgtggc
     7381 gctcccagtt ttcttgcttt gcctgtgccg ttttacgtag tgcgacgtaa cacgccccgc
     7441 tgccgccatg atgcggtagc gcggtacaat atgcctggac atcatcaaat tcggtcaacc
     7501 agcgcgccac atagctgcgg acaatattgg catgcgattt atcttcccgc cctttaccat
     7561 gaataatcag cacattacgc aaaccatccg ccagggcttg ttgaatgaaa ctgaacacca
     7621 ttttgcggca ctcttccacc ggctggcgca aaagattcag gctcgcctgt tgcggatatt
     7681 taccactgcg cagcttatcc agcaccccat gttgcaaccc ttcccgccga aactccagcg
     7741 gctgacttag cgggatgatg tcgagaaatc cggtggtgag gaaattatca agttgcagcg
     7801 tgtcgatacg ctgcggcgca cgttggttac gcgttggatg ccagtggaca tctgtagcac
     7861 gtttcagcgg ctggacatct tccatggcgt caagaaacag cgatttgtcg tcaaggttca
     7921 tgtaacctcc tatgcacctt cacacgtcgt gattcagacg ggttaattgt tcatacttac
     7981 tgcatcgttc ccttcacaga ttgaaacaat actctacttc atacctgtga gtatctgcgc
     8041 aggcggggca ttttatacta gcctgttagc agtatcgaac agtttagccc gagtttaatt
     8101 cagcacaggc tccgatcagg atgatttatt catagcagga accaacaaaa atgcgataaa
     8161 taacagacca ctgattagca gaaaggtatc cgaaaatgcc atagtttgcg cctccctcag
     8221 catcaattga ctcaataatt tggttgaagc cagtatttcc gatgtctggt cacttccact
     8281 gcgatcaaaa aacaaagcac ttctggaaat gaagtcattc accgtatggg gaacagaaac
     8341 cattttttca cccattctcg aaaaatgaaa attagttcga ttattgagaa tagagccaca
     8401 cagggcaata ccaatcgcgc ccccaagatt acgtgttaaa ttaaatacac cagaggctaa
     8461 cttcagccgt tcttttggaa ttcctcccag cgttaatgtt acgatggggg ccatcgcaaa
     8521 ctgctgcgaa atccctctaa ttgcctgtgg aaacagtaac tcctgccatc cccattcgtg
     8581 ggttataggg gtaaaaagat acattgaaaa cacaaatcca cccagccccg ccatcaacag
     8641 ccatcgcaga tttatttttt tacttaacca gaagtaaaag ggaacagaaa ataactggaa
     8701 aatacctgtt gtgcaaacgg caaaacctat ctcttcggca tttaatccgc gaacctgccc
     8761 caa
//
LOCUS       DACBVI010000169         8684 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.170, whole genome shotgun sequence.
ACCESSION   DACBVI010000169 DACBVI010000000
VERSION     DACBVI010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.170"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            140..1537
                     /gene="ulaA"
                     /locus_tag="IU325_19585"
     CDS             140..1537
                     /gene="ulaA"
                     /locus_tag="IU325_19585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP5811207.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            1553..1858
                     /gene="ulaB"
                     /locus_tag="IU325_19590"
     CDS             1553..1858
                     /gene="ulaB"
                     /locus_tag="IU325_19590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HAP5811208.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            1868..2332
                     /gene="ulaC"
                     /locus_tag="IU325_19595"
     CDS             1868..2332
                     /gene="ulaC"
                     /locus_tag="IU325_19595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HAP5811209.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2346..2996
                     /gene="ulaD"
                     /locus_tag="IU325_19600"
     CDS             2346..2996
                     /gene="ulaD"
                     /locus_tag="IU325_19600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP5811210.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            3006..3860
                     /locus_tag="IU325_19605"
     CDS             3006..3860
                     /locus_tag="IU325_19605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HAP5811211.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     DRLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            3860..4546
                     /locus_tag="IU325_19610"
     CDS             3860..4546
                     /locus_tag="IU325_19610"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP5811212.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKAADMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARGINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4675..4950)
                     /gene="yjfY"
                     /locus_tag="IU325_19615"
     CDS             complement(4675..4950)
                     /gene="yjfY"
                     /locus_tag="IU325_19615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAP5811213.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHPILIREPGSNGNMHASADIYR"
     gene            5277..5672
                     /gene="rpsF"
                     /locus_tag="IU325_19620"
     CDS             5277..5672
                     /gene="rpsF"
                     /locus_tag="IU325_19620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP5811214.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            5679..5993
                     /gene="priB"
                     /locus_tag="IU325_19625"
     CDS             5679..5993
                     /gene="priB"
                     /locus_tag="IU325_19625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HAP5811215.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            5998..6225
                     /gene="rpsR"
                     /locus_tag="IU325_19630"
     CDS             5998..6225
                     /gene="rpsR"
                     /locus_tag="IU325_19630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP5811216.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6267..6716
                     /gene="rplI"
                     /locus_tag="IU325_19635"
     CDS             6267..6716
                     /gene="rplI"
                     /locus_tag="IU325_19635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP5811217.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(6787..7581)
                     /locus_tag="IU325_19640"
     CDS             complement(6787..7581)
                     /locus_tag="IU325_19640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAP5811218.1"
                     /translation="MTLPTTIYSFPAYSPRFSSTDKPVKLKFHQYARATLLSNRGRDH
                     NCDGRHTVEIHKLDLSDWQAFNKLATRCNTYDGITMNGDNSFGWNHEATLDNIHAQKY
                     NKAYAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSSS
                     AAIYSGLGFERVPQGRDIRCDHMHLTPANHPELWTQENGEWIYLRN"
     gene            7823..8122
                     /locus_tag="IU325_19645"
                     /pseudo
     CDS             7823..8122
                     /locus_tag="IU325_19645"
                     /inference="COORDINATES: protein motif:HMM:NF037007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8024..8638)
                     /gene="tnpA"
                     /locus_tag="IU325_19650"
                     /pseudo
     CDS             complement(8024..8638)
                     /gene="tnpA"
                     /locus_tag="IU325_19650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513092.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         2141 a         2047 c         2300 g         2196 t
ORIGIN      
        1 tatccatgtg gaattatttg cgggtcgcgt cacatttaat cataaataat cttgttgtga
       61 ttacttttga aaattagagt gagtgcacaa cattccgggt gtgtggaata cccggttacc
      121 tcttcttcag gagatcgtta tggagatcct ctacaacatc tttaccgtgt tttttaacca
      181 ggtcatgacc aatgccccgt tgttgctggg tattgtgacc tgtctgggct acatcctact
      241 gcgcaaaagt gtcagcgtta ttattaaagg cacgattaaa accataattg gtttcatgtt
      301 gttgcaggca gggtccggca tcctcaccag caccttcaaa ccggtggtgg cgaaaatgtc
      361 cgaagtctac ggcattaacg gcgcaatttc cgatacttac gcatcgatga tggcaaccat
      421 cgaccgcatg ggcgatgcct atagctgggt aggttacgcg gtgttgttag cgctggcact
      481 gaacatctgt tacgtgctgc tgcgtcgcat taccggtatt cgtaccatca tgctgactgg
      541 tcatatcatg ttccagcagg cggggctgat tgccgttacg ttgtttattt tcggctactc
      601 catgtggacg accattatct gcacggcgat tctggtttcg ctctactggg gcattacctc
      661 caacatgatg tacaagccga ctcaggaagt gacggacggc tgcggtttct ccatcggtca
      721 ccagcaacag tttgcatcat ggattgccta taaggttgcg ccgttcctcg gcaaaaaaga
      781 ggaaagcgtt gaagacctca aactgccagg ctggctgaat attttccacg acaacattgt
      841 ctccacggcg attgtcatga ccatcttctt tggtgccatt ctgctctcct tcggtatcga
      901 caccgtgcag gcgatggcag gcaaagtgaa ctggacggtc tacatcctgc aaactggttt
      961 ctccttcgcg gtggcgatct tcatcatcac tcaaggcgtg cgcatgtttg tggcggaact
     1021 ctctgaagcg tttaacggta tctcccagcg cctgatccct ggtgcggttc tggcgattga
     1081 ctgtgcggct atctatagct tcgcgccgaa cgccgtggtc tggggcttta tgtggggcac
     1141 catcggtcag ctgattgcag ttggcatcct ggtcgcctgc ggttcctcga tcctgattat
     1201 tcccggcttt atcccgatgt tcttctccaa cgccaccatc ggcgtgttcg ctaaccactt
     1261 cggcggctgg cgtgcggcgc tgaagatttg tctggtgatg gggatgatcg aaatctttgg
     1321 ttgcgtctgg gcggtgaaac ttaccggtat gagtgcctgg atgggtatgg cggactggtc
     1381 gattctggca ccaccgatga tgcagggctt cttctccatc ggtatcgcct ttatggccgt
     1441 catcattgta attgcactgg cttatatgtt cttcgctggc cgcgcgctgc gcgcagaaga
     1501 agatgcagaa aaacaactgg cagaacagtc tgcttaataa ggagttttga ttatgaccgt
     1561 acgtattctg gctgtgtgtg gcaacggaca aggcagttcc atgatcatga agatgaaagt
     1621 ggaccagttt ttaacccaat caaacattga ccacacggta aacagctgcg cggttggcga
     1681 atacaaaagc gagttgagcg gcgcggatat catcatcgct tctacgcaca ttgcgggcga
     1741 aatcaccgtg accggcaaca aatacgtggt tggcgtgcgc aacatgctct ctcctgctga
     1801 ctttggcccg aaactgctgg aagtgatcaa agcgcatttc ccgcaggatg tgaagtaagg
     1861 acgcgccatg aaattacgtg attcgctggc ggaaaataaa tccatccgcc tgcaggctga
     1921 agctgagaca tggcaggacg cagtaaaaat cggcgttgac ctgctggtag cggcggatgt
     1981 tgtcgagccg cgttactacc aggcgattct ggatgcggtt gaacagcacg gtccgtactt
     2041 tgtgctcgca ccaggcctgg caatgccgca cggtcgcccg gaagagggcg ttaagaaaac
     2101 cggttttgcg ctagtgacgc tgaaaaagcc gctggagttc aaccacgaag ataacgatcc
     2161 ggtggatatc ctcatcacca tggcggcggt cgatgccaac actcaccagg aagtgggcat
     2221 catgcagatc gtcaatctgt ttgaagatga agagaatttc gaccgtttac gtgcctgccg
     2281 taccgagcag gaagtactgg atttaattga ccgcaccaac gcggcagctt aagaaggaat
     2341 tgaacatgtc attaccgatg ttgcaggtcg cgctggacaa ccagactatg gatagcgcct
     2401 atgaaaccac tcgcctgatt gccgaagaag tcgacattat cgaagtgggt accattctgt
     2461 gcgtgggcga aggcgtgcgt gcggttcgcg atctgaaagc gctttacccg cacaaaatcg
     2521 tgctggcaga cgccaaaatt gccgatgcag gcaaaatcct ttcgcgtatg tgcttcgaag
     2581 ccaacgctga ctgggtgacc gtgatttgct gtgcggatat caacaccgcc aaaggcgcgc
     2641 tggacgtggc aaaagagttt aacggcgacg tgcagatcga actgactggc tactggacct
     2701 gggaacaggc gcaacagtgg cgcgacgcag gtattcagca ggtggtttat caccgcagcc
     2761 gtgacgcgca ggccgcaggc gtggcgtggg gcgaagctga cattaccgcg atcaaacgtc
     2821 tgtccgatat gggctttaaa gtgaccgtca ccggtggcct ggcgctggaa gatctgccgc
     2881 tgttcaaagg cattccgatt catgtgttta tcgctggtcg cagtatccgt gatgccgctt
     2941 ctccggttga agctgcgcgt cagttcaaac gttccattgc cgaactatgg ggctaaggag
     3001 cggatatgtt gtccaaacaa atcccgcttg gcatctatga aaaagcgctc cccgccgggg
     3061 agtgctggct ggaacggctg caactggcaa aaacgttagg cttcgatttt gtcgagatgt
     3121 cggtagatga aacagacgat cgcctgtcgc gcctcgactg gagccgcgag cagcgtctgg
     3181 cgctggtgaa tgcgattgtt gaaaccggcg tgcgtgtacc gtctatgtgc ctttctgctc
     3241 atcgtcgttt cccgctgggc agcgaagatg acgcggtacg ggcgcagggg ctggagatta
     3301 tgcgtaaagc tatccagttc gcccaggacg ttggcattcg cgtaatccaa ctggcgggct
     3361 atgacgttta ctatcaggaa gccaataacg aaacgcgtcg tcgtttccgt gacggcctga
     3421 aagagagcgt tgagatggca agccgcgcgc aggtgacgct ggcgatggag atcatggatt
     3481 acccgttgat gaactccatc agtaaggcgt tgggttacgc gcactatctc aacaacccgt
     3541 ggttccagct ctacccggat atcggcaacc tgtcggcgtg ggacaacgac gtgcagatgg
     3601 aattgcaggc cggaattggg catatcgtcg cggtgcatgt gaaagacacc aaaccaggcg
     3661 tcttcaaaaa cgtgccgttt ggcgaaggtg tggtggattt cgaacgttgc ttcgaaacgc
     3721 tcaaacagag tggctattgc gggccgtacc tgattgagat gtggagcgaa acggcggaag
     3781 acccggcggc agaagtggcg aaagcgcgtg attgggtgaa agcgcgcatg gcgaaagcgg
     3841 gcatggtgga ggcggcataa tgcaaaagct aaaacagcag gtatttgaag ccaacatgga
     3901 tctgccgcgc tacgggctgg tgacctttac ctggggcaac gtcagcgcca ttgaccgcga
     3961 acgcgggctg gtggtgatca agcccagcgg cgttgcctac gaaaccatga aagcggccga
     4021 tatggtggtg gttgatatga gcggcaaggt ggtggaaggg gaatatcgcc cgtcttccga
     4081 caccgcgacg catctcgaac tctaccgtcg ttatccgtcg cttggcggca ttgtccatac
     4141 ccactccact catgctaccg catgggcgca ggcggggctg gcgatcccgg cgttaggcac
     4201 tacgcacgcc gactacttct ttggtgatat cccgtgtacg cgcggattaa gcgaagaaga
     4261 ggtgcagggt gagtacgaac tgaacaccgg caaagtgatt atcgaaacgc tgggcaacgc
     4321 cgagcctctg catacaccgg ggattgtggt gtaccagcac gggccgttcg cctgggggaa
     4381 agatgctcac gatgcggtgc ataacgcggt ggtgatggaa gaagtggcaa aaatggcgtg
     4441 gattgcccgc ggcattaacc cacaattgaa tcacatcgac agctacctca tgaataaaca
     4501 cttcatgcgt aagcacggtc ccaacgctta ttacgggcag aagtaaaaca cgcgctgcgg
     4561 aaatttcctt cttcgggaga taactggtct aattccgcag ccgtttttca aaaaaagccc
     4621 cctgcgaagg gggcaaagca aactatggca atgtttcgtt ggttatacct ggtgctagcg
     4681 ataaatatcc gcgctggcgt gcatattgcc gttacttccc ggttcccgta tcaggatcgg
     4741 atggtagtac gttgcgccct gcgcatctgt ctcttcattt aatgcctgac gtgcttcact
     4801 ttcagtggcg aagttatggt tgatataaat cacgcctaag ctttgcacat cgtccatatt
     4861 tctggcctgg tggttattaa tttcaatggc tgcccatgta tttgcactta gcaaaagcac
     4921 agccagaagg gctaaaacac gactgaacat agatacctcc tcaacggctg actttgtgtg
     4981 ctctcctctg tgatgatctt ctgatttaat tttaatcaat gataaagaag ttgatggtga
     5041 ccatttctga tgcagttgtt caaaaaaaca ccatgatgaa gtgtgatgaa cttcaaatca
     5101 gcgtgttagt acgatttggt gaaagatgag atttatttgt cggctggcct tgagtttagc
     5161 ggggtatgtc agtactataa cgcgtcattt ttcagccgac ctttaacacg ttccttgcct
     5221 ccccgggatt cggctgaccc agacaggagg ctgaataatc cgtaaggagc aattcgatgc
     5281 gtcattacga aatcgttttt atggtccatc ctgaccagag cgaacaggtt ccgggcatga
     5341 tcgagcgcta cactgctgcc atcactggtg cagaaggcaa gatccaccgt ctggaagact
     5401 ggggccgccg tcagctggct tacccgatca acaaactgca caaagcacac tacgttctga
     5461 tgaacgttga agctccgcag gaagtgatcg atgagctgga aactaccttc cgcttcaacg
     5521 atgccgttat ccgcagcatg gttatgcgta ccaagcacgc tgttaccgaa gcatctccga
     5581 tggttaaagc gaaagacgag cgccgtgagc gtcgcgatga tttcgcaaac gaaaccgctg
     5641 atgatgctga agctggggat tctgaagagt aatttctgat gaccaaccgt ctggtgttgt
     5701 ccggcaccgt gtgcaggact ccccttcgaa aggtcagtcc atcaggaatt cctcactgcc
     5761 agttcgtgct tgagcatcgt tctgtgcagg aggaagccgg ttttcaccgg caggcgtggt
     5821 gccaaatgcc cgttattgtt agcggacacg aaaaccaggc cattactcac agtataacgg
     5881 tcggcagtcg cataaccgtt caggggttca tttcatgcca caaggcaaag aacggactga
     5941 gcaaaatggt tttgcatgcc gagcagattg aattgataga ttctggagac tagccatatg
     6001 gcacgttatt tccgtcgtcg caagttctgc cgtttcaccg cggaaggcgt tcaagagatc
     6061 gactataaag atatcgctac gctgaaaaac tacatcaccg aaagcggtaa gattgtccca
     6121 agccgtatca ccggtacccg tgcaaaatac cagcgtcagc tggctcgcgc tatcaaacgc
     6181 gctcgctacc tgtccctgct gccgtacact gatcgccatc agtaatcggt cacggtccat
     6241 taatacgact ttgagaggat aaggtaatgc aagttattct gcttgataaa gtagcaaacc
     6301 tgggtagcct gggtgatcag gtaaacgtta aagcgggcta tgctcgtaac ttcctggtac
     6361 cgcagggtaa agctgttcca gctaccaaga aaaacattga attcttcgaa gcacgtcgcg
     6421 ctgaactgga agctaaactg gctgaagttc tggcagctgc taatgctcgc gctgagaaaa
     6481 tcaatgcact ggaaactgtt accatcgcgt ctaaagctgg cgacgaaggt aaactgttcg
     6541 gttccatcgg tactcgcgac atcgctgacg ctgtaactgc agctggcgtt gaagtggcta
     6601 agagcgaagt tcgtctgccg aacggcgttc tgcgtaccac tggcgaacac gaagtgagct
     6661 tccaggttca cagcgaagta ttcgcgaaag tgatcgtaaa cgtagtagct gaataattcg
     6721 ttattcaacg agacgtaaaa acgccgacta ctggtcggcg ttttgctttc tatttttcgt
     6781 caggtattag tttcgcaagt agatccattc accattctct tgtgtccata attcaggatg
     6841 gtttgctggc gttagatgca tatgatcgca ccttatgtct cggccctgcg gcactcgctc
     6901 gaaacccaaa cccgagtaga ttgctgctga acttgataag gaacgcagac caattttcaa
     6961 atcatggggt aaatcagaat acgcgtttag caatgcagca atgatgaatc cgagggcatt
     7021 agaaacgcct gtaacagtgc gaagttggtc gatataaaga tcgattgttg gtagttcaga
     7081 tgaccagcaa ttaggcattt gggcaggaag ggttacaacg tatcctatcg tttggccggt
     7141 atagtctgga gtaccataac ctggacccaa aacaacctcg tgtattgtgt atttactgtt
     7201 gggttcgaag gattcgacat cttgcaataa atatttcagt tctgcggtta acctcgcacc
     7261 cgcgtaagct ttattgtatt tctgggcgtg aatattatct aatgtagctt catggttcca
     7321 tccaaaagaa ttatcaccat tcattgttat tccatcgtaa gtattacacc tcgtcgctaa
     7381 cttattgaat gcttgccagt ctgagagatc tagtttgtgg atctcaacag tatgtctgcc
     7441 atcgcaatta tggtctcttc cacgatttga aagtaatgta gcgcgtgcgt attgatgaaa
     7501 ttttaatttt actggtttgt cggtggacga aaaacggggt gaatatgcag gaaatgaata
     7561 aatagttgtt ggtaatgtca ttttattgtt gcctgtttaa tatttttaca aatatcgcag
     7621 ttgaatgttc aaagaaaaag agttgatatt aataaaaata agtttgtttt ctgaagtggt
     7681 gaaaatagcg gatagtaaaa gatattaata tcaaaaatcg ctcatcacgg atataatgat
     7741 gcacccaggg agggagatga ctgacaacgc cttacaccat acattcttgt tgtgcggtat
     7801 gtaattattc tacgctgtcg gtatcggtgc gttcgttaat cagcaggtta accagccaca
     7861 aaaatccatt gaaaaaatca tcgattttac ctggggttat tatattgcta atccctggtt
     7921 tttaaaaatt gtgcatagtg aaaaccaaag taaaggtgtg cattatgcaa aattacagcg
     7981 tctcttagaa attaatcatg cgcacttgca gttaatggaa tccttattgg atgaagggaa
     8041 gaaacacaat atttttaagc cagatatcga cccattgcag gtgtatatta atattgctgc
     8101 gcttggggga tattatttga tcaaccagca tacgcttggc ctggtttatc cggtgcgccg
     8161 taaaaccccg tccttcaggg cggggatata aggcgcggtt ttccacctga ccaggtgttt
     8221 gctgttgttc aatgtattgg cggatgacgg atattggcgc acctccgcag ctactggcaa
     8281 aatagccagg actccacaaa acgcctttgt agtaatacct gactgcaata tctggtcgat
     8341 ctcgtcgcag tcacctaccc aatacgcctt tgaggctgtt taccagactg gatatcgcca
     8401 gtttgggagg atagttaatt aacaaatgga cgtgatctgg ttcgccatcc atttcaacca
     8461 gttcagcttc aaaatcagca catacctttg aaaagtaagt gcgtagtttt tctttcgcgt
     8521 catggtcaaa aatctggcgt cggtatctgg tgacaaagac caggtgaaca tgcatcagga
     8581 aaacacaatg ctttccacgc cggatatcgg tttctttttt catagaccaa agtatcattt
     8641 gacctgtgaa acgattacag gcatttaaat tccagttaag acca
//
LOCUS       DACBVI010000170         8590 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.171, whole genome shotgun sequence.
ACCESSION   DACBVI010000170 DACBVI010000000
VERSION     DACBVI010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.171"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            309..1292
                     /locus_tag="IU325_19655"
     CDS             309..1292
                     /locus_tag="IU325_19655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HAP5811219.1"
                     /translation="MSERHLPDDQSSTIDPYLITSVRQTLAEQGAALQNLSKQLDSGQ
                     YQRVLNLIMNCKGHVILSGMGKSGHVGRKMSATLASTGTPSFFIHPAEAFHGDLGMIT
                     PYDLLILISASGETDEILKLVPSLKNFGNRIIAITNNGNSTLAKNADAVLELHMANET
                     CPNNLAPTTSTTLTMAIGDALAIAMIHQRKFMPNDFARYHPGGSLGRRLLTRVADVMQ
                     HDVPAVQLDASFKTVIQRITSGCQGMVMVEDAEGGLAGIITDGDLRRFMEKEDSLTSA
                     TAAQMMTREPLTLPEDTMIIEAEEKMQKHRVSTLLVTNKANKVTGLVRIFD"
     gene            1364..2512
                     /locus_tag="IU325_19660"
     CDS             1364..2512
                     /locus_tag="IU325_19660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export inner-membrane
                     protein KpsE"
                     /protein_id="HAP5811220.1"
                     /translation="MLIKVKSAVSWMRARLSAISLADIQKHLAKIIILAPMAVLLIYL
                     AIFSQPRYMSESKVAIKRSDDLNSGSLNFGLLLGASNPSSAEDALYLKEYINSPDMLA
                     ALDKQLNFREAFSHSGLDFLNHLSKDETAEGFLKYYKDRINVSYDDKTGLLNIQTQGF
                     SPEFALKFNQTVLKESERFINEMSHRIARDQLAFAETEMEKARQRLDASKAELLSYQD
                     NNNVLDPQAQAQAASTLVNTLMGQKIQMEADLRNLLTYLREDAPQVVSARNAIQSLQA
                     QIDEEKSKITAPQGDKLNRMAVDFEEIKSKVEFNTELYKLTLTSIEKTRVEAARKLKV
                     LSVISSPQLPQESSFPNIPYLIACWLLVCCLLFGTLKLLLAVIEDHRD"
     gene            2536..4212
                     /locus_tag="IU325_19665"
     CDS             2536..4212
                     /locus_tag="IU325_19665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid transporter"
                     /protein_id="HAP5811221.1"
                     /translation="MKLFKSILLIAACHAAQASAAIDINADPNLTGAAPLTGILNGQQ
                     SDTQNMSGFDNTPPPAPPVVMSRMFGAQLFNGTSADSGATVGFNPDYILNPGDSIQVR
                     LWGAFTFDGALQIDPKGNIFLPNVGPVKVAGVSNSQLNTLVTSKVKEVYQSNVNVYAS
                     LLQAQPVKVYVTGFVRNPGLYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVR
                     SNVNLYDFLLNGKLGLSQFADGDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEA
                     LSWARPKPGATHITIMRKQGLQKRSEYYPISSAPGRMLQNGDTLIVSTDRYAGTIQVR
                     VEGAHSGEHAMVLPYGSTMRAVLEKVRPNSMSQMNAVQLYRSSVAQRQKEMLNLSLQK
                     LEEASLSAQSSTKEEASLRMQEAQLISRFVAKARTVVPKGEVILNESNIDSVLLEDGD
                     VINIPEKTSLVMVHGEVLFPNAVSWQKGMTTEDYIEKCGGLTQKSGNARIIVIRQNGA
                     AVNAEDVDSLKPGDEIMVLPKYESKNIEVTRGISTILYQLAVGAKVILSL"
     gene            4222..4962
                     /gene="kdsB"
                     /locus_tag="IU325_19670"
     CDS             4222..4962
                     /gene="kdsB"
                     /locus_tag="IU325_19670"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HAP5811222.1"
                     /translation="MSKAVIVIPARYGSSRLPGKPLLDIVGKPMIQHVYERALQVAGV
                     AEVWVATDDQRVEKAVQAFGGKAIMTRNDHESGTDRLVEVMHKVEADIYINLQGDEPM
                     IRPRDVETLLQGMRDDPALPVATLCHAISAEEATEPSTVKVVVNTRQDALYFSRSPIP
                     YPRNAEKARYLKHVGIYAYRRDVLQNYSQLPESMQEQAESLEQLRLMSAGINIRTFEV
                     AATGPGVDTPACLEKVRALMAQELAENA"
     gene            4959..6986
                     /locus_tag="IU325_19675"
     CDS             4959..6986
                     /locus_tag="IU325_19675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HAP5811223.1"
                     /translation="MIGIFSSGIWRIPHLEKFLAQPCQKLSLLRPVPQEVDAIAVWGH
                     RPSAAKPVAIAKAAGKPVIRLEDGFVRSLDLGVNGEPPLSLVVDDCGIYYDASKPSAL
                     EKLVQDKAGNTALISLAREAMHTIVTGDLSKYNLAPAFVADESERSDIVLVVDQTFND
                     MSVTYGNAGPHEFAAMLEAAMAENPQAEIWVKVHPDVLEGKKTGYFADLRATQRVRLI
                     AENVSPQSLLRHVSRVYVVTSQYGFEALLAGKPVTCFGQPWYAGWGLTDDRHPQSAVL
                     SARRGSATLEELFAAAYLRYCRYIDPLTGAASDLFTVLQWLQLQRSHQQQRNSYLWAP
                     GLTLWKSAILKPFLRTATNRLSFTRRCTAATACVVWGVKGEQQWRAEAQRNSLPLWRM
                     EDGFLRSSGLGSDLLPPLSLVLDKRGIYYDATRPSDLEVLLNHSQLTLAQQMRAGKLR
                     QRLVESKLSKYNLGADFSLPAEAKDKKIILVPGQVEDDASIKTGTVSIKSNLELLRTV
                     RERNPHAYIIYKPHPDVLVGNRKGNIPAELIAELADYQAMDADIIQCIQRADEVHTMT
                     SLSGFEALLHGKQVHCYGLPFYAGWGLTVDEHHCPRRKRRLTIADLIYQALIVYPTYI
                     HPTRLQPITVEEAAEYLIQTPRKPMFITRKKAGRVIRYYRKLIMFCKVRFG"
     gene            7021..8253
                     /locus_tag="IU325_19680"
     CDS             7021..8253
                     /locus_tag="IU325_19680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAP5811224.1"
                     /translation="MQGNALTILLSGKKYLLLQGPMGPFFNDVAEWLESLGRNAVNVV
                     FNGGDRFYCRHRQYLAYYQTPKEFPGWLRDLHRQYDFDTILCFGDCRPLHKEAKRWAT
                     SKGIRFLAFEEGYLRPQFITVEEGGVNAYSSLPRDPDFYRKLPDMPAPHVENLKPSTM
                     KRIGHAMWYYLMGWHYRREFPRYRHHKSFSPWYEARCWVRAYWRKQLYKVTQRKVLPR
                     LMNDLDQRYYLAVLQVYNDSQIRNHSNYNDVRDYINEVMYSFSRKAPKESYLVIKHHP
                     MDRGHRLYRPLIKRLSKEYGLGERVIYVHDLPMPELLRHAKAVVTINSTAGISALIHN
                     KPLKVMGNALYDIKGLTYQGHLHQFWQAEFKPDMKLFKKFRGYLLVKTQVNWVYYGDF
                     ISEYESKDNNFTFIPQKR"
BASE COUNT         2251 a         2050 c         2169 g         2120 t
ORIGIN      
        1 atagtcaagt aggaaacatt ttaataaatg ataaaaatcc taaattcctt gttcataatg
       61 taggggtgtg ttaaataggt gggcaaaatt tggttcccct tctcgcctgt aatattgcag
      121 catcttcgca cctccatgag acattgcgac ttaataagaa ggtgataagt cctgcatata
      181 agcatggact gaccatggtt ttatattcat taaaaatttt gttacaaccc attgatttag
      241 cataaataaa ttatagtggg ttcgggtttg ttgtgactgt ggcattattt ccgtgcaaag
      301 gagctgatat gtctgaaaga catttacctg atgaccagag cagtactatc gatccatatc
      361 taattacctc tgttcgccag actctggcag aacaaggcgc agcattacaa aacttgtcta
      421 aacaactgga ttccgggcag taccagcgtg tccttaattt gataatgaac tgtaaaggtc
      481 acgttattct ttcgggaatg ggtaaatcag ggcatgtcgg tcgtaaaatg tcagcgacgc
      541 tggcctctac gggtacgcct agtttcttta ttcatcctgc agaagctttc catggcgatc
      601 tgggcatgat tacgccttac gatcttctga tccttatttc tgccagcggt gaaacggatg
      661 aaatcctcaa gctagttcct tcactgaaaa atttcggcaa ccgaattatc gccattacca
      721 ataatggaaa ttccacgctg gcgaaaaatg ctgatgccgt gctggaactc cacatggcga
      781 atgaaacctg cccgaataat cttgcaccaa caacgtctac cacgctgacg atggcgatcg
      841 gcgatgcgct ggcgattgcc atgatccacc aacgcaaatt tatgccgaat gattttgcgc
      901 gctatcaccc gggcggttca ttaggtcgtc gcctgctgac ccgcgttgct gatgtcatgc
      961 agcatgatgt tcctgcggta cagctggatg cgtcatttaa aaccgtgatt caacgtatca
     1021 ccagcggatg ccagggaatg gtgatggtag aagacgcaga aggtgggcta gcgggcatta
     1081 tcaccgacgg tgacctgcgt cgctttatgg aaaaagagga ttctctgaca tccgccacgg
     1141 ctgcgcagat gatgacacgt gaaccgctga cgctaccgga agacaccatg atcattgaag
     1201 cggaagaaaa aatgcaaaag caccgcgtct caacgttatt ggtgaccaac aaggcaaata
     1261 aagtcactgg ccttgtgcgc attttcgact aattaagcaa cggggtgcga gaggttagtc
     1321 tctcattgag tttgcgcccc actgaaactt tgataatcgt tacatgttga taaaagtgaa
     1381 gtctgccgta tcctggatgc gtgctcgtct gtctgccatc tcactggcag atatccaaaa
     1441 acacctggcg aaaatcatca ttctggcacc gatggcggtg ctgctgatct atctggctat
     1501 cttcagccag cctcgctata tgagcgagtc gaaagtcgcc attaaacgct cggatgattt
     1561 aaacagcggc agcctgaatt ttggtctgct tctgggtgcc tctaacccca gttccgcaga
     1621 agatgcgttg tatctgaaag agtacatcaa ctcgccggat atgctggcgg cgctggataa
     1681 gcaactaaat tttcgtgaag cgtttagcca cagcgggctc gattttctta atcatcttag
     1741 caaggatgaa accgcagaag gcttcctgaa gtactacaag gaccgtatca acgtctcgta
     1801 tgacgataaa accggattac tgaatattca gacgcagggc tttagcccgg aatttgcgct
     1861 taagtttaac caaaccgtgc tgaaagagtc agagcgcttt atcaatgaga tgtcacatcg
     1921 catcgcgcgt gaccagcttg cctttgcaga aacggagatg gaaaaggcac gccagcgtct
     1981 ggacgccagc aaagcggaat tgctctctta tcaagacaac aacaacgttc tggatccaca
     2041 ggcacaggca caggcggcga gcacgttagt gaatacgctg atgggccaga agatccagat
     2101 ggaagcggac ctgcggaact tgctgacgta tctgcgtgag gacgccccgc aagttgtgag
     2161 tgcgcgtaat gcgattcagt cattgcaggc acaaattgac gaagaaaaaa gcaaaatcac
     2221 agcgccgcag ggtgacaagc taaaccgtat ggcggtggat tttgaagaaa tcaaatcaaa
     2281 agtagagttc aacaccgagc tgtacaaact gaccctgacc tccattgaaa agacccgtgt
     2341 agaagcggct cgtaagctca aggtgctgtc agtgatcagt tcgccacagt tgccgcagga
     2401 atcgtctttt ccaaatatcc cttatttgat cgcctgctgg ttactggtgt gctgcctgct
     2461 gttcggcacc ctgaaactgt tgctggctgt tattgaagat caccgagact aacgctgtcg
     2521 ctgaatgagt ttgtgatgaa attatttaaa tcaattttac tgattgccgc ctgtcacgcg
     2581 gcgcaggcca gcgcggccat tgatattaac gctgacccaa accttacagg agccgcgccg
     2641 cttaccggta ttctgaacgg gcaacagtcg gatacgcaaa acatgagcgg cttcgacaat
     2701 accccgccgc ccgcaccgcc ggtggtcatg agccgcatgt ttggtgctca acttttcaac
     2761 ggcaccagcg cggatagcgg tgcgacggta ggattcaacc ctgactatat tctgaatccg
     2821 ggtgatagca ttcaggttcg cttgtggggt gcgttcacct ttgatggtgc attacagatt
     2881 gatcccaaag gtaatatttt tctgccaaac gttggtccgg tgaaagttgc cggggttagc
     2941 aatagtcagt tgaacactct ggttacatca aaagtgaagg aagtgtacca gtccaacgtc
     3001 aacgtctacg cctccttatt acaggcgcag ccagtaaaag tgtacgtgac cggatttgtg
     3061 cgtaatcctg gtctgtatgg cggtgtgacg tctgattcgt tactcaatta tctgatcaag
     3121 gctggcggcg ttgatccaga gcgcggaagt tacgttgata ttgtggtcaa gcgcggtaac
     3181 cgcgtgcgct ccaacgtcaa cctgtacgac ttcctgctga acggcaaact ggggctttcg
     3241 cagttcgccg atggtgacac catcatcgtc gggccgcgtc agcatacttt cagcgttcag
     3301 ggcgatgtct ttaacagcta cgactttgag ttccgcgaaa gcagcattcc cgtaacggaa
     3361 gcgttgagct gggcgcgccc taagcctggc gcgactcaca ttacgattat gcgtaaacag
     3421 gggctgcaaa aacgcagcga atactatccg atcagttctg cgccaggccg tatgttgcaa
     3481 aatggcgata ccttaatcgt gagcactgac cgctatgccg gcaccattca ggtgcgggtt
     3541 gaaggcgcac actccggtga acatgccatg gtactgcctt atggttccac tatgcgtgcg
     3601 gttctggaaa aagtccgccc gaacagcatg tcgcagatga acgcggttca gctttatcgc
     3661 tcatcagtag ctcagcgtca gaaagagatg ctgaatctct cgctgcaaaa actggaggaa
     3721 gcctcacttt ctgcccagtc atccaccaaa gaagaagcca gcctgcgaat gcaggaagca
     3781 caactgatca gccgctttgt ggcgaaagca cgcaccgtgg ttccgaaagg tgaagtgatc
     3841 ctcaacgaat ccaatattga ttctgttctg cttgaagatg gcgacgtcat caatattccg
     3901 gagaaaacat cgctggttat ggttcatggc gaagtgctgt tcccgaacgc ggtgagctgg
     3961 cagaagggta tgaccaccga ggattacatc gagaaatgtg gtggcctgac gcagaaatcg
     4021 ggtaacgcca gaattatcgt cattcgtcag aacggtgcag ccgtcaacgc agaagatgtg
     4081 gattcactca aaccgggcga tgagattatg gttctgccga aatatgaatc gaaaaacatt
     4141 gaagttaccc gtggtatttc caccatcctc tatcagctgg cggtgggtgc aaaagtgatt
     4201 ctgtctttgt aaggagttga aatgagcaaa gctgttattg tcatcccggc tcgttatggc
     4261 tcatcgcgtc tgccgggtaa gccactgctc gatattgttg gtaaaccgat gatccagcat
     4321 gtttacgagc gtgcgttaca ggtggcgggc gttgcggaag tttgggtggc aacagacgat
     4381 cagcgtgttg aaaaggccgt gcaggcattt ggcgggaaag ccatcatgac gcgcaacgat
     4441 catgaatccg gcaccgatcg gctggtcgag gtgatgcata aagtcgaggc agatatctac
     4501 attaacctgc agggcgacga accaatgatt cgcccgcggg atgtagaaac gctgttacaa
     4561 ggaatgcgtg atgaccccgc gttgccagtg gcaacgctat gccacgcgat ttctgccgaa
     4621 gaagcgaccg agccaagcac ggtaaaagtg gtggtaaata cccgccagga tgcgctttat
     4681 ttcagccgtt cgcctattcc gtatccgcgt aatgctgaaa aagcgcgcta cctgaaacac
     4741 gttggtattt acgcttatcg tcgcgatgtg ctgcaaaact acagccagtt accggagtcc
     4801 atgcaggagc aggcagaatc actggagcag ctgcggttga tgagcgcggg gatcaacatc
     4861 cgcacatttg aggttgccgc aaccggtccg ggcgtcgaca ccccagcatg cctggaaaaa
     4921 gtgcgcgccc tgatggcaca ggaactggct gaaaacgcat gattggcatt ttctcgtccg
     4981 gtatctggcg tattccgcat ctggagaaat ttctggcgca accgtgccag aaactttctc
     5041 tgctgcgccc tgttccgcaa gaagttgatg ctatcgccgt gtggggacat cgtcccagcg
     5101 cggcgaaacc agtcgccatc gccaaagcag cgggaaaacc cgtcattcgt ctggaagatg
     5161 gatttgtgcg ttcgctggat cttggcgtca atggcgagcc gccgctttct ctggtggtgg
     5221 atgattgtgg catttactac gatgccagca agccttcagc actggagaaa ctggtacagg
     5281 ataaagccgg aaatacagct ctgataagcc tggccagaga agcgatgcac accatcgtga
     5341 ccggggattt gtcgaaatat aacctggcac ctgcgtttgt ggctgatgag tcagaacgtt
     5401 cagacatcgt tctggttgtc gatcagacat ttaatgatat gtcagtgacg tatggcaatg
     5461 ctggcccgca tgagtttgct gccatgctgg aagccgcgat ggcggaaaat cctcaagccg
     5521 aaatttgggt gaaggtgcat ccggatgtcc tggaaggaaa gaaaacaggt tatttcgctg
     5581 atctgcgcgc cacgcaacga gtacgtttga ttgccgagaa tgtcagcccg cagtcgctgt
     5641 tgcgacacgt ttcccgggtt tacgtcgtga catcccaata cggctttgaa gccttgctgg
     5701 caggaaaacc agtaacatgt ttcggtcagc cctggtatgc aggctggggc ttaaccgacg
     5761 accgccatcc gcagtccgct gtgttatctg cccgacgcgg ttctgccacg ctggaagaac
     5821 tttttgccgc agcatacctg cgttactgtc gctatatcga cccgctaacg ggggccgcaa
     5881 gcgatctgtt taccgtactg caatggctgc aattacagcg cagccatcag caacagcgta
     5941 atagctattt atgggcgcca ggcttaacgc tgtggaagtc agcgatcctg aaacctttct
     6001 tgcgaacagc gacaaaccga ctgagtttta cacgtcgctg tactgcggcc accgcttgcg
     6061 tggtatgggg tgtaaaggga gaacagcaat ggcgagccga agcacagcga aactcactgc
     6121 cgttatggcg aatggaagat ggttttctgc gttcatccgg acttggctct gacctgttgc
     6181 cgccgctatc gttggtactg gataaacgcg ggatctacta tgacgccacg cgccccagcg
     6241 acctggaagt actgcttaat cacagccagt taacgctggc acagcagatg cgagctggaa
     6301 aattacgcca gcggctggtt gagagcaaac tgagcaaata caacctgggg gccgatttct
     6361 ctctgcctgc cgaggccaaa gataaaaaaa tcatcctggt gccgggtcag gtagaagacg
     6421 atgcatctat taaaacaggc actgtctcga ttaagagcaa ccttgagtta ttacgcacag
     6481 tacgtgaacg taacccgcac gcctacatta tttataaacc gcacccggat gtattagtgg
     6541 ggaatcgcaa gggcaatatt ccggcagaac tgattgctga actcgctgat tatcaggcaa
     6601 tggacgcaga tattattcaa tgtattcagc gcgcagatga agtgcacacc atgacatcat
     6661 tgtccgggtt tgaagcgtta ttacatggca agcaagttca ttgttacggc ctgcccttct
     6721 atgccggttg gggtttaacc gttgatgaac atcactgccc gcgccgcaag cgcagattaa
     6781 cgatagcaga cttgatctat caggcgttga ttgtttatcc aacctatatc cacccaacac
     6841 gactacaacc tattacggtt gaggaagctg cggaatattt aatccagacg ccgcgtaagc
     6901 cgatgtttat tacccgaaaa aaagcggggc gagtaatacg ctattaccgc aaattaatta
     6961 tgttctgcaa ggtcagattt ggctgaacaa tttcatagtt gactattaca cagattattt
     7021 atgcaaggca acgcactaac cattttatta tcgggtaaaa aatatctgct tttgcaggga
     7081 ccgatgggac cttttttcaa tgacgtcgcc gaatggttag agtcattagg ccgtaacgct
     7141 gtgaatgtag tattcaacgg cggggatcgt ttttactgcc gccatcgaca atacctggct
     7201 tactaccaaa cgccgaaaga gtttcccggt tggttgcgag atctccaccg gcaatatgac
     7261 tttgacacca tcctctgctt tggtgactgc cgcccattgc acaaagaagc aaaacggtgg
     7321 gcaacgtcga aagggatccg ctttctggca tttgaagaag gatatttacg cccgcaattt
     7381 ataaccgttg aagaaggcgg agtgaacgca tattcatcgc taccgcgcga cccggatttt
     7441 tatcgtaagt tgccagatat gcctgcgccg cacgttgaga atttaaaacc ttcaacgatg
     7501 aaacgtattg gccatgcaat gtggtattac ctgatgggct ggcattaccg tcgtgagttt
     7561 cctcgctacc gccaccacaa atcgttttcc ccctggtatg aggctcgttg ctgggttcgt
     7621 gcatactggc gcaagcaact ttacaaggta acacagcgta aggtattgcc gaggttaatg
     7681 aatgacctgg atcaacgtta ttatcttgcc gttttgcagg tatataacga tagccaaatt
     7741 cgtaaccaca gcaattataa cgatgtgcgt gactatatta atgaagtcat gtactcattt
     7801 tcgcgtaaag cgccgaaaga gagttatttg gtgatcaaac atcatccgat ggatcgtggt
     7861 cacagactct atcgaccatt aattaagcgg ctaagtaagg aatatggctt aggtgagcga
     7921 gtcatttatg tgcacgatct cccgatgccg gaactattac gccatgcaaa agcggtggta
     7981 acaattaaca gtacggcggg gatctctgcg ctgattcata acaaaccact caaagtgatg
     8041 ggcaatgccc tgtacgacat caaaggctta acgtatcaag ggcatttgca ccagttctgg
     8101 caggccgagt ttaaaccgga tatgaaactg tttaagaagt ttcgggggta tttattggtg
     8161 aagacgcagg tgaattgggt ttattatggg gattttattt ctgaatacga gagtaaggat
     8221 aataacttta catttatacc acaaaagcga tagaaccccc atttcaaaat caaacagaaa
     8281 aacatatttc tctagagagc aataatttat tgacaataat gaaaacctag ataacagcat
     8341 atccttcaat attattaaca tcaatgtaga acattccccc ttccagatat ttctatacca
     8401 tatcctaagc tttgataatc agcgtgctgt tcgcaataca tctagtacat ccatctcatc
     8461 actatgcaaa ggccagtatt catcgtgttt tctgaaccta ttgttaaatc gtgctcataa
     8521 agaacctcgc aatcgtcttc atatttaaac tttatactga aatttctcat ttacatagat
     8581 atgcctatta
//
LOCUS       DACBVI010000171         8490 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.172, whole genome shotgun sequence.
ACCESSION   DACBVI010000171 DACBVI010000000
VERSION     DACBVI010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.172"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            176..553
                     /locus_tag="IU325_19685"
     CDS             176..553
                     /locus_tag="IU325_19685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811225.1"
                     /translation="MDECFINITPTSLSFKNAELSDLSYKNARLELTLICPAIKSEVK
                     LSFDWIHSFRVTDEGDLLGMSGIHGINQTGIYRVVNSAYFAWFTMQSCEIYKNNVIEH
                     YVIATSNDVIDVLSTVSPQIQYD"
     gene            669..1469
                     /gene="ydcF"
                     /locus_tag="IU325_19690"
     CDS             669..1469
                     /gene="ydcF"
                     /locus_tag="IU325_19690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein"
                     /protein_id="HAP5811226.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEMPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            1666..3105
                     /gene="aldA"
                     /locus_tag="IU325_19695"
     CDS             1666..3105
                     /gene="aldA"
                     /locus_tag="IU325_19695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAP5811227.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISVGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAIAMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(3147..4148)
                     /gene="gap"
                     /locus_tag="IU325_19700"
     CDS             complement(3147..4148)
                     /gene="gap"
                     /locus_tag="IU325_19700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP5811228.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDIVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            4337..4867
                     /gene="cybB"
                     /locus_tag="IU325_19705"
     CDS             4337..4867
                     /gene="cybB"
                     /locus_tag="IU325_19705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HAP5811229.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHEMLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            5112..5285
                     /locus_tag="IU325_19710"
     CDS             5112..5285
                     /locus_tag="IU325_19710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811230.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(5357..5506)
                     /gene="hokB"
                     /locus_tag="IU325_19715"
     CDS             complement(5357..5506)
                     /gene="hokB"
                     /locus_tag="IU325_19715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HAP5811231.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            5905..7545
                     /gene="trg"
                     /locus_tag="IU325_19720"
     CDS             5905..7545
                     /gene="trg"
                     /locus_tag="IU325_19720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HAP5811232.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRSVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILSKAVKIRSARANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FIVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVAMAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLIEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVLAEPRGAGEQVSFATV"
     gene            complement(7584..>8490)
                     /locus_tag="IU325_19725"
     CDS             complement(7584..>8490)
                     /locus_tag="IU325_19725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP5811233.1"
                     /translation="FSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSKTLNELE
                     QLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDVVRVGAL
                     PTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSDPELMTG
                     LNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDALVQSQGC
                     KIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGAGEPIGI
                     LTRVDATFSSGCQLMINAIRKSMPF"
BASE COUNT         2204 a         1944 c         2145 g         2197 t
ORIGIN      
        1 tgaatgtatt gcagatgttc cccctgaaaa aaatggttga tgactggaca aggtaatttc
       61 aaatcacagt aatgtccatc gattatacat ggtacgtaaa cagactcgca ccgtttttgc
      121 agaaggttaa tgtgtcgtct atcgagagca gtctcatata tgaaggggta ttaacatgga
      181 tgaatgtttt ataaatatca ctccgacttc attgtctttt aaaaatgccg aactatcaga
      241 tttaagctat aaaaatgcca ggcttgaact taccctcata tgtccggcga tcaagtcaga
      301 agttaagtta tcatttgact ggatacattc atttcgtgtt actgacgaag gtgatttgtt
      361 aggaatgtca ggaattcacg gtattaacca aacggggatc tatagagtag tgaattccgc
      421 ctactttgca tggtttacta tgcaaagttg tgaaatttat aaaaataatg tgatcgagca
      481 ttatgtcatt gccacgtcaa atgatgttat tgatgtttta tcaacagttt ctccgcaaat
      541 acagtatgac taaaaccagt gtctggttcg cgatggcgag gtagaaaaag catcatcttc
      601 ctttcagagt gctccgcttc tcattattat ctcacgcagt attcttaagg gaacgataag
      661 gaggaaccat gaacattacc ccgtttccga cgctttcgac ggcaactata gatgccataa
      721 atgttatcgg acagtggctg gcgcaggatg atttctccgg tgagatgccg tatcaggccg
      781 attgcgtgat ccttgcaggc aatgcggtta tgccgactat cgatgcggca tgtaagattg
      841 cccgcgatca gcaaattcct ctactgatta gtggtggtat cggtcactcg acaacttttt
      901 tgtatagcgc tatcgcacag catccgcact acaacactat ccgcaccact ggcagagcag
      961 aagccaccat cctggcggat atcgctcatc agttctggca tattccgcat gaaaaaatct
     1021 ggattgaaga ccagtcaaca aactgcggtg aaaacgcacg ctttagcatc gcgctattga
     1081 atcaggccgt agaacgagtt catacggcta tcgttgttca ggaccccacc atgcagcggc
     1141 gcacgatggc gacgttccgc cgtatgaccg gggacaatcc agatgcacct cgctggttaa
     1201 gttatcccgg attcgttcct cagctaggaa acaacgcaga cagtgtaatc tttgttaatc
     1261 cgttgcaagg attatggccg gttgagcgtt atctctcact actcactggc gagctgccgc
     1321 gtttacgcga tgatagcgat ggctacggtc cccgcgggcg agattttatc gttcacgttg
     1381 attttccggc agaagtcatc catgcatggc aaacgctgaa acacgatgcg gtgctcatcg
     1441 aggcgatgga aagtcgctcg ttacgttaaa aattgcccgt ttgtgaacca cttgtttgca
     1501 aacgggcata actcctgcct tttatttctg cctcttattc cttttacact tgtttttatg
     1561 aagcccttca cagaattgtc ctttcatgat gccgtctctc tgatgattga tgttaattaa
     1621 caatgtattt accaaaaaca aacatataaa tcacaggagc cgctcatgtc agtacccgtt
     1681 caacatccta tgtatattga tggacagttt gttacctggc gtggagacgc atggattgat
     1741 gtggtaaacc ctgctacaga ggctgtcatt tcccgcattc ccgatggtca ggccgaggat
     1801 gcccgtaagg caatcgatgc agcagaacgt gcacaaccag aatgggaagc gttgcctgct
     1861 attgaacgcg ccagttggtt gcgcaaaatc tccgtcggga tccgcgaacg cgccagtgaa
     1921 atcagtgcgc tgattgttga agaagggggc aagatccagc agctggctga agtcgaagtg
     1981 gcttttactg cggactatat cgattacatg gcggagtggg cacggcgtta cgagggcgag
     2041 attattcaaa gcgatcgtcc aggagaaaat attcttctgt ttaaacgtgc gcttggtgtg
     2101 actaccggca ttctgccgtg gaacttcccg ttcttcctca ttgcccgcaa aatggctccc
     2161 gctcttttga ctggtaatac cattgtcatt aaacccagtg aatttacgcc aaacaatgcg
     2221 attgcattcg ccaaaatcgt cgatgaaata ggccttccgc gcggcgtgtt taaccttgtt
     2281 ctggggcgtg gtgaaaccgt tgggcaagaa ctggcgggta acccaaaggt cgcaatggtc
     2341 agtatgacag gcagcgtctc tgcaggtgaa aagatcatgg cgactgcggc gaaaaacatc
     2401 accaaagtgt gcctggaatt ggggggtaaa gcaccagcta tcgtaatgga cgatgccgat
     2461 cttgaactgg cagtcaaagc catcgttgat tcacgcgtca ttaatagtgg gcaagtgtgt
     2521 aactgtgcag aacgtgttta tgtacagaaa ggcatttatg atcagttcgt caatcggctg
     2581 ggtgaagcgt tgcaggcggt tcaatttggt aaccccgcag aacgcaacga cattgcgatg
     2641 gggccgttga ttaacgccgc ggcgctggaa agggtcgagc aaaaagtggc gcgcgcagta
     2701 gaagaagggg cgagagtggc gttgggtggc aaagcggtag aggggaaagg atattattat
     2761 ccgccgacat tgctgctgga tgttcgccag gaaatgtcga ttatgcatga ggaaaccttt
     2821 ggcccggttc tgccagtggt cgcatttgac acgctggaag atgctatcgc aatggcgaat
     2881 gacagtgatt acggcctgac ctcatcaatc tatacccaaa atctgaacgt cgcgatgaaa
     2941 gccattaaag ggctgaagtt tggtgaaact tacatcaacc gtgaaaactt cgaagctatg
     3001 caaggcttcc acgccggatg gcgtaaatcc ggtattggcg gcgcagatgg taaacatggc
     3061 ctgcatgaat atctgcagac ccaggtggtt tatttacagt cttaatgagt gaaagaggcg
     3121 gaggtttttt cctccgcctg tgcgcgtcag agtttagcga atttttcgag ggtgcgaata
     3181 agctgcgtga cgaagccata ttcgttatcg taccaggcga ccgttttcac cagttgtaaa
     3241 tcgcccacgg cggtaatttc ggtttgcgtg gcatcaaaca ccgaaccgaa atggctgcca
     3301 atgatatcgg aagagactat ttcttcatcg gtataaccaa atgactcgtt attggtggtt
     3361 gcttgtttaa gtgcgttatt cacctcttcg gcagtcactt tttttccgag aatcgatacc
     3421 agttcagtga cggaacctgt tttcaccggc acgcgttgcg catgaccttt cagtttgccg
     3481 ctcagttctg ggatcaccag accaatggct tttgccgccc ccgtagtgtg gggaatgata
     3541 ttttctgccg ctgcgcgtga agcacgtaaa tctttaccac gcgggccatc caccagtgac
     3601 tgagtgccgg tataggcatg aatggtcgtc atcgtgccga cttctattcc gaaactgtcg
     3661 tgcaaggctt tggccatcgg cgcaaggcag ttagtggtgc atgacgccac ggaaacaatg
     3721 gtgtcgttgc catccagagt gtcgtcattg acgttataaa cgatagtttt catttcaccg
     3781 gcaggggcgg aaatcaacac cttcttcgca ccagcatcaa gatgcgcctg cgatttctcg
     3841 gcggaggtat aaaagccagt acattcgaca ataatttctg cccctttcgc tttccacgga
     3901 atatttttag cctctttttc ggcgtaaacc gcgatacttt tcccatcaac gataagtgaa
     3961 tcttccgtaa aatcaacgct ccaggggaac ggtccatagt ttgaatcatg tttcagcagg
     4021 taggcgagaa tttttgggga agtgagatca ttaatagcga caatgtctat gttgcttttg
     4081 acttcaagta atcgacgcaa caccagtcga ccgatacgac caaaaccgtt aataccaact
     4141 ttactcatgg tcttctcctg tcaggaacgt tcggatgaaa attgatcctt tccaggctta
     4201 gaccaggatg gcgggatggg caatctacat tcttgccgtg aaacgtaacg tactgaaaac
     4261 gggtgaacaa tatttaatga aattttaaga aaagcccgtt atgttaacgg aaaattatgt
     4321 taaagcagga aatgttatgg aaaataaata ctcaaggtta caaatcagca ttcactggct
     4381 ggtctttttg ctggttattg cagcgtattg cgcaatggag tttcgggggt tcttcccacg
     4441 tagcgatcgg ccactcatca acatgattca tgtttcctgt ggcatatcaa ttctcgtgct
     4501 gatggtcgtt cgtcttttgt taaggctgaa atacccaacc ccgccgatta tacctaagcc
     4561 aaaaccgatg atgacgggac tggcgcattt gggacatttg gtgatttatc tcctgtttat
     4621 tgcgctgcca gtgattggtt tggtgatgat gtataaccga ggcaacccgt ggtttgcgtt
     4681 tggtttgacg atgccttatg catcagaggc caatttcgaa cgggttgata gcttaaagtc
     4741 gtggcatgaa atgctggcga atctgggata ttttgtcatc gggttgcacg ctgcggctgc
     4801 actggcacac cactatttct ggaaggacaa cacacttcta cgcatgatgc cgcgtaaacg
     4861 ttcctgaagg atatttaaag aaaacgcctg tactaaaacc gacccgtggt acaggcgaag
     4921 aatacgggtc tacatcggaa gcgcctatta tatttatttg tatgacaaat aaaaccataa
     4981 tccttatcca tacgtccatc tggcttatct ttaatcaatt cgcccgatct ttgatctcat
     5041 caacggtatc aaaataaacc agcgtataac gttcaaatcg aacataagat tcgataaacc
     5101 atggaggtta tatgaaaaaa ctggcactta tcttgtttat gggaacgctt gtttcctttt
     5161 atgccgatgc cgggcgcaaa ccctgttctg gttcgaaagg ggggatctca cactgtacgg
     5221 caggcggcaa atttgtctgt aatgatggtt ctattagtgc atcgaaaaaa acatgcacta
     5281 actgaagtgt aaaaggggtg ccatgagaaa atgaatctgc tagtcagatg cgcggggaaa
     5341 atccccgcgc ttgcccttac ctggacgtgc aggccatgag cgcagcaacc tccttatcac
     5401 cgtcccggaa ccgcagttcg tagagcgttt gtcgggtcag gagtgtgaat gtcagaatcg
     5461 taatgcagat aatgagcaga cacaccacca gagggttgcg cttcatagcc tttctccttg
     5521 cctgatggcg ggtaagaggc taacatctga attgcttggt tcattcgttg gcctcggttg
     5581 atagaaatat cggtcggggc cttcgtcttt ctgattcccg gttagcctga aaacagaaag
     5641 tctcaggcac ccgcaggcat cctatgaggt ttccttaggg acgaaaataa tcacttcacg
     5701 aaattgcgtg ctgttttcca gaatttttcg tcattcggtt tagccagttt aatcattcgt
     5761 tactctcttc attccaatgg cattattctt ctctgccata attgttgtaa aaatgtgacg
     5821 caaagaggtt tttggtcgta agaaattacc gtcaagtgcc gatgactttc tatcaggagt
     5881 aaacctggaa gagagacaac ggtaatgaat acaactccct cacagcgatt aggttttttg
     5941 catcacatca ggctggttcc gttatttgcc tgcattcttg gcggtatctt agttctattc
     6001 gcattaagtt cagccctggc cggctatttc ctttggcagg ccgatcgcga tcagcgtgat
     6061 gttactgcgg agattgagat tcggaccggg ttagcgaaca gttcagattt tttgcgttca
     6121 gcccggatca atatgattca ggctggtgct gcgagtcgta ttgctgaaat ggaagcaatg
     6181 aagcgaaata ttgcgcaagc cgaatcggag attaaacagt cgcagcaagg ttatcgtgct
     6241 tatcagaatc ggtcggtgaa aacacctgct gatgaagccc tcgacactga attaaatcaa
     6301 cgcttccagg cttatatcac gggtatgcaa ccgatgttga aatatgccaa aaatggcatg
     6361 tttgaagcga ttatcaatca tgaaagtgag cagatccgac cgctggataa tgcttatacc
     6421 gatattttga gcaaagccgt taagatacgt agcgccagag ccaaccaact ggcggaactg
     6481 gcccatcagc gcacccgcct gggtgggatg ttcatgattg gcgcgtttgt gcttgccctg
     6541 gtcatgacgc tgataacatt tatagtgcta cgtcggatcg tcattcgtcc actgcaacat
     6601 gccgcacaac ggattgaaaa aatcgccagt ggcgatctga cgatgaatga tgaaccggcg
     6661 ggtcgtaatg aaatcggtcg cttaagtcgt catttacagc aaatgcagca ttcactgggg
     6721 atgacagtag ggactgttcg acagggtgcg gaagagattt atcgtggcac cagcgaaatt
     6781 tcagctggca atgcggacct gtcatctcgc accgaagaac aagcggcggc tatcgaacaa
     6841 actgccgcca gcatggagca acttactgcg acggtgaaac agaatgcgga taacgcgcat
     6901 catgccagca aactggcgca agaggcttct attaaagcca gcgatggcgg gcagacggtt
     6961 tccggtgtag taaaaacgat gggcgctatc tccacgagtt cgaagaaaat ttctgagatc
     7021 accgccgtca tcaacagtat tgctttccag acgaatattc tggcactgaa tgctgccgtt
     7081 gaagccgcgc gagcgggtga gcaagggcgt ggatttgccg ttgtcgccag cgaagtacgg
     7141 acactcgcaa gccgcagcgc tcaggcggcg aaagagattg aaggcttgat cagtgaatca
     7201 gtcaggttaa ttgacctggg gtcggatgag gtggcaatgg cagggaaaac catgagcact
     7261 attgttgatg ccgtcgcgag tgtcacacat atcatgcagg aaatcgccgc tgcctcggat
     7321 gaacaaagca gaggcataac tcaggttagc caggcgattt ctgaaatgga taaggtgacg
     7381 caacagaatg cttctctgat agaagaggcc tcagcggcgg cggtgtccct tgaagaacag
     7441 gcggcacgat taactgaggc ggtggacgta ttccgtctga acaaacattc tgtgttggca
     7501 gaacctcgcg gagcgggtga acaagttagt ttcgctacgg tgtgaaaatg ttcaaggaag
     7561 ggatcgacag atcccttcac ctttcagaac ggcattgatt ttcgaatagc gttaatcatc
     7621 aactggcaac cagaagagaa cgttgcatct acgcgggtca gtattccaat cggttcgcct
     7681 gcaccatgtc ccggaacagg cagggccacc agcgtggcat gacgcaggtc gtcttttacc
     7741 gcgccagaag ggacaaacca cacgtagtcg tattcaaccg taagttgacg agatagcgaa
     7801 gcagacagcg tttcgataca acccgaagga attttacatc cctggctctg cactaatgca
     7861 tctgaatgct ggcgtggcgc agtgccttct ggtgatacaa cgaccggcca ttccagcacc
     7921 cggcttagcg ttacgttctc ctgaagtagc ggatgattgg ggcggacaac cagcttcagc
     7981 gattcaagaa acagcagttc gtaattaagc ccggtcatca gttcaggatc tgacatccga
     8041 ccaatgccga tatcgatttc cccggttttc aaacccgcca gaatcatagg gttactcatt
     8101 gtcgcaactt gcaaggtcgt ttctttttgt tgctgatgaa actgacctat aaccgaaggt
     8161 aatatcccca gtgccgcagt aggtagtgca ccaaccctga cgacatcatt attaagacct
     8221 tctttacgat gaagcgactg tccggcagtg ttgatggcgt caagaactct gactgcatgc
     8281 gttaaaaatt gttcgccggg taaggtaagt tgcgccccct gacgaccacg ctcaaacaag
     8341 cgagcgcccg tcagttgctc cagttcattc aatgttttag agagcgcagg ttgactcaaa
     8401 ttaagggttt cagccgcgcg ccccaaagtt ccttgttgtg cgacagctac gaatgtatga
     8461 aggtggcgca aacggatgcg ctgactaaac
//
LOCUS       DACBVI010000172         8465 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.173, whole genome shotgun sequence.
ACCESSION   DACBVI010000172 DACBVI010000000
VERSION     DACBVI010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.173"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..384)
                     /locus_tag="IU325_19730"
     CDS             complement(<1..384)
                     /locus_tag="IU325_19730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005764212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HAP5811234.1"
                     /translation="MSYERVRFTASNITQNETRFYSMSIPTDILKNCCFVSTRDEDPE
                     KGFQRTLDEKRAREIADYIDSGHGTIPSSIILSAQPEAEVQIVGKGRTMEFSVHPKAF
                     LILDGQHRVYGFSLAKSAVRVPVVIY"
     gene            complement(895..1929)
                     /locus_tag="IU325_19735"
     CDS             complement(895..1929)
                     /locus_tag="IU325_19735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP5811235.1"
                     /translation="MSKKKGKTPQPAAKTMTASSPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
     gene            complement(1929..3701)
                     /locus_tag="IU325_19740"
     CDS             complement(1929..3701)
                     /locus_tag="IU325_19740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP5811236.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNVANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRMLERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            3875..4729
                     /locus_tag="IU325_19745"
     CDS             3875..4729
                     /locus_tag="IU325_19745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HAP5811237.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVAELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQDVTDRADETSQAFSRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            4788..5861
                     /locus_tag="IU325_19750"
     CDS             4788..5861
                     /locus_tag="IU325_19750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HAP5811238.1"
                     /translation="MRQETRFKFNAYLSRVAELNGIDAGDVSKKFTVEPSVTQTLMNT
                     MQESSDFLTRINIVPVSEMKGEKIGIGVTGSIASTTDTAGGTERQPKDFSKLASNKYE
                     CDQINFDFYIRYKTLDLWARYQDFQLRVRNAIIKRQSLDLIMAGFNGVRRAETSDRSS
                     NQMLQDVAVGWLQKYRNEAPARVMSKVTDEEGHTTSEVIRVGKGGDYASLDALVMDAT
                     NNLIEPWYQEDPDLVVIVGRQLLADKYFPIVNKEQDNSEMLAADVIISQKRIGNLPAV
                     RVPYFPADAMLITKLENLSIYYMDDSHRRVIVENPKLDRVENYESMNIDYVVEDYAAG
                     CLVEKIKVGDFSTPTKVTAEPGA"
     gene            5865..6608
                     /locus_tag="IU325_19755"
     CDS             5865..6608
                     /locus_tag="IU325_19755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP5811239.1"
                     /translation="MTSPAQRHMMRVSAAMTAQRDAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDTRLLTETLALTATAD
                     MPDEVRAKLHKITGLFLRDAGDAAGALAHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRAPRKTRSVTPAKRGRPKKKAS"
     gene            6708..7217
                     /locus_tag="IU325_19760"
     CDS             6708..7217
                     /locus_tag="IU325_19760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HAP5811240.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFTRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            7217..7420
                     /locus_tag="IU325_19765"
     CDS             7217..7420
                     /locus_tag="IU325_19765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP5811241.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVETVLAANPGMAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            7424..7705
                     /locus_tag="IU325_19770"
     CDS             7424..7705
                     /locus_tag="IU325_19770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP5811242.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            7705..8202
                     /locus_tag="IU325_19775"
     CDS             7705..8202
                     /locus_tag="IU325_19775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HAP5811243.1"
                     /translation="MPVINTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            8217..>8465
                     /locus_tag="IU325_19780"
     CDS             8217..>8465
                     /locus_tag="IU325_19780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysA"
                     /protein_id="HAP5811244.1"
                     /translation="MKKLSLSLMLNVSLALMLALSLIYPQSVAVSFVAAWAILATVIC
                     VVAGGVGVYATEYVLERYGRELPPESLAVKIVTSLFLQP"
BASE COUNT         1907 a         2256 c         2421 g         1881 t
ORIGIN      
        1 ataaattaca actggtactc gaacagcaga tttagctagt gaaaagccat aaacgcgatg
       61 ttgaccatca agaattagaa aagctttagg atgaacagaa aattccatag ttcgcccttt
      121 tcccacgatt tgaacctctg cctcaggttg agcagataaa ataattgatg aggggattgt
      181 tccatgcccg ctatctatat aatcagctat ttctctagca cgtttttcat ctagtgttcg
      241 ctgaaaacct ttttcaggat cttcatctcg cgtcgaaacg aagcagcaat ttttcaatat
      301 atctgtaggt atagacatcg agtagaaacg cgtttcgttt tgtgtgatgt tgctggcagt
      361 gaatcttact ctttcataac tcatttgaac aattccttca ttactgttaa atgaacaata
      421 gtttataaaa cttagtgtaa caacagcaag ttatacatcc agttcgttgg atgttttaac
      481 accaatggat ggttctcttt agcggaagac ctacaatgtg ataaatatca tgggggataa
      541 tcccagtaaa cattttgtta cgttttttaa atatgagctt ctcctaagcg taggatgcat
      601 cattttgcat gaatattctt cgattgtgtg gcataacaaa acactggtga agcctgagtt
      661 aaactaaaag ttgccactgc attaaaaccg ccccatgaag cgggcaggcg aggcggggaa
      721 agcactgcgc gctggcggtg gtgcggattt tattttttca gcgtctcagc gcgtcgtgac
      781 ggcgcttagt ctgcccgttg aggcgttggt gtgtctgcgg ggtgttttgt gcggtggtga
      841 gcgtgtgagg gggtgatgac ggggtgtaaa aaagccgccc gcaggcggcg atgttcagcc
      901 gttgtcagtg tccagtgagt agtttttaaa tcggatgacc tcctgaccga gccagccgtt
      961 tatttcgcgg atcctgtcct gtaacgggat aagctcattg cggacaaaga cctttgccac
     1021 tttctcaata tcacccagcg acccgacgtt ctccggcttg ccccccatca actgaaaggg
     1081 gatgcgatgc gcgtccagca ggtcagcggc gctggctttt ttgatattaa aaaaatcgtc
     1141 cttcgttgcc acttcactga gcgggataat tttaatgccg tcggctttcc cctgtggggc
     1201 atagagaaac agatttttaa agttattgcg gcctttcgac ttaaccatgt tttcgcgaag
     1261 catttcgata tcgttgcgat cctgcacggc atcggtgacg tacatgatgt atccggcatg
     1321 agcgccgttt tcgtaatact tgcggcggaa cagcgtggcc gactcattca gccaggcaga
     1381 gttaagggcg ctgagatatt ccggcaggcc gtacagctcc tgattaatat ccggctccag
     1441 caggtgaaac acggagccgg gtgcgaaggc tgtcggctcg ttgaaggacg gcacccacca
     1501 gtaaacatcc tcttccacac cacggcgggt atattttgcc ggtgaggttt ccagtctgat
     1561 gaccttaccg gtggtgctgt aacgcttttc cagaaacgca ttaccgaaca ccagaaaatc
     1621 cagcacaaag cggctgaaat cctgttgcga aagccacgga tgcgggataa acgttgaagc
     1681 cagaatattg cgtttgacgt aaatcggtga gctgtgatgc acggcagcac gcaggctttt
     1741 tgccagaccg gtaaagctga ccggtggctc ataccatctg ccgttactga tgcactcgac
     1801 gtaatccaga atgtcacggc ggtcgagtac cggcaccggc tcaccaaagg tgaatgcctc
     1861 cattttcggg gagctggcgg tcattgtttt tgccgcaggt tgcggtgttt tccctttttt
     1921 cttgctcatc agtaaaactc cagaatggtg gatgtcagcg gggtactgat accggcggtg
     1981 agtggctcat ttaacagggc gtgcatggtc gcccaggcga ggtcggcgtg gctggcttcc
     2041 tcgctgcggc tggcctcata ggtggcgctg cgtccgctgc tggtcatggt cttgcggata
     2101 gccataaacg agctggtgat gtcggtggcg ctgacgtcat attccagaca gccacggcga
     2161 ataacgtctt ttgccttgag caccattgcg gttttcattt ccggcgtgta gcggatatcg
     2221 cgcgcggcgg gatagaacga gcgcacgagc tggaacacgc cgacaccgag gccggtggca
     2281 tcaatgccga tgtattcgac gttgtatttt tcggtgagtt tgcggatgga ttccgcctgg
     2341 gtggcaaagt ccatgccttt ccactggtga cgctcaagca ttctgaattt gccaccggcc
     2401 accaccggcg gtgccagtac cacgcatccg gcgctgtcgc cacggtgtga cgggtcgtaa
     2461 ccaatccaaa ccggacggga gccgaacgga ttggcggcaa acggcgcata gtcttcccat
     2521 tcttccagcg tgtcgaccat gcagcgttgc agctcctcga acgggaacac cgacgccttg
     2581 tcgtcaacaa attcacacat gaacaggttt ttaaaatcgt cggcgctgtt ttcacgtttg
     2641 agctgctcaa tgtcgaacag tgtgcagcca cctttcaggg cgtcctcaat ggtgacaatc
     2701 tgccgccact gaccgtccgc acagagaaga cctccggcaa gggcgttatg actgacgtcg
     2761 atttccacgc gttcggcggc gctggcgcgt ccccggttga acagttcacc cgaccagaac
     2821 ggataggcgt cgtgcgccag cgtggacggg gtggagaaat aggtcgatcg caggtgactc
     2881 tgtgaggcca tacctgatgc caccttacgc agcacctgaa aattcgggat ccagaaaatc
     2941 tcgtcgacgt acaggtcgcc gttatggctc tgcgcggtgt tggagttggt gccgagaaaa
     3001 atcagttttg cgccgttatt gccaaggaca atcgggtcac cggtcaggtc aacatcaacc
     3061 agacgggcaa aggcgatgat gtattcgcgg aacacatacg cctgcgtttt actggccgac
     3121 agaaaaatct ggttatgacc ggttttcagg gcgcgcagca gcgcctcgcg ggaaaaataa
     3181 aacgtcgcgc caatctggcg ggatttcagg atatcgcgga tgcggtgctc aagcccggcg
     3241 cgataccagt gcaactgata atcgaaagac tgctcaaaga aaatctgctc cagcttttcg
     3301 atggcctcgt cactgaaaaa attctttttc ggtttgcgcc gcccgccttt gttgcggtta
     3361 gcgacgttcg gattaaggtc tgcctcgttg ccggtctggc tgtagcggtt tacccgtgcc
     3421 agtcgttcaa tctggcgtcc gagcaggtca atttccttga aatcaccgcc ggttttctgc
     3481 ggtttgatga tgagctgggt cagccgcgct tccagactca tttcgacacg gctgatgggg
     3541 gcaacgctgt cccagccgtc gcgctgtttc cagctctgca ccgtcgggcg tttcatctgc
     3601 aacatggcgg caatctgcgg cacggaaaat ccctgccagt acagcagcgc cgcctgacga
     3661 cgcgggtcgt gtaaaagagt ggtgtctgtg gtgatggtca tgaatacctc gccgtgatga
     3721 atacacggca aggctactga gtcgcgcccc gcgattcgct aaggtgctgt tgtgtcagtg
     3781 ataagccatc cgggactgat ggcggaggat gcgcatcgtc gggaaactga tgccgacatg
     3841 tgactcctct aatcactatt caggactcct gacaatggca aaaaaagtct caaaattctt
     3901 tcgtatcggc gttgagggtg acacctgtga cgggcgtgtc atcagtgcgc aggatattca
     3961 ggaaatggcc gaaacctttg acccgcgagt ctatggttgc cgcattaacc tggaacatct
     4021 gcgcggcatc ctgcctgacg gtatttttaa gcgttatggc gatgtggccg aactgaaggc
     4081 cgaaaagatt gacgatgatt cggcgctgaa aggcaaatgg gcgctgtttg cgaaaatcac
     4141 cccgaccgat gaccttatcg cgatgaacaa ggccgcgcag aaggtctaca cctcaatgga
     4201 aattcagccg aactttgcca acaccggcaa atgttatctg gtgggtctgg ccgtcaccga
     4261 tgacccggca agcctcggca cggaatacct ggaattctgc cgcacggcaa aacacaaccc
     4321 cctgaaccgc ttcaaattaa gccctgaaaa cctgatttca gtggcaacgc ccgttgagct
     4381 ggaatttgaa gacctgcctg aaaccgtgtt caccgccctg accgaaaagg tgaaatccat
     4441 ttttggccgc aaacaggcca gcgatgacgc ccgtctgaat gacgtgcatg aagcggtgac
     4501 cgctgtcgct gaacatgtgc aggaaaaact gagcgccact gagcagcgcc ttgctgagat
     4561 ggaaaccgcc ttttccgcac ttaagcagga tgtgactgac agggcggatg aaaccagcca
     4621 ggcattcagc cgcctgaaaa acagtctcga ccacaccgaa agtctgaccc agcagcgccg
     4681 cagcaaggcc accggcggtg gcggtgacgc cctgatgacg aactgctgac cggcgtcagc
     4741 cagtccggga aaaccttcac gattaaccct taatttcagg aaaaactatg cgccaggaaa
     4801 cccgctttaa atttaatgcc tacctgtccc gtgttgccga actgaacggc atcgacgccg
     4861 gtgatgtgtc gaaaaaattc accgttgaac cgtcggtcac ccagaccctg atgaacacca
     4921 tgcaggagtc ctctgacttt ctgacccgca tcaacattgt gccggtcagc gaaatgaaag
     4981 gggaaaaaat tggtattggt gtcaccggct ccatcgccag caccacagac accgccggtg
     5041 gcaccgagcg tcagccgaag gacttctcga agctggcgtc aaacaagtac gaatgcgacc
     5101 agattaactt cgatttttat atccgctaca aaacgcttga cctgtgggcg cgttatcagg
     5161 atttccagct ccgtgtccgt aacgccatta tcaaacgcca gtcccttgat ttaatcatgg
     5221 ccggttttaa cggcgtgagg cgtgccgaaa cctctgaccg cagcagtaac cagatgctgc
     5281 aggatgtggc ggtcggctgg ctgcagaaat accgcaatga agccccggcg cgcgtgatga
     5341 gcaaggttac tgacgaggaa ggtcacacga cctctgaggt catccgcgtg ggtaagggcg
     5401 gtgattatgc cagcctcgat gcactggtga tggatgcgac caacaacctg attgagccgt
     5461 ggtatcagga agaccctgac cttgtggtga ttgtggggcg tcagctactg gcggacaagt
     5521 atttccccat cgtcaacaag gagcaggaca acagcgagat gctggccgct gacgtcatca
     5581 tcagccagaa acgcatcggt aacctgccgg cggtacgcgt cccgtacttc ccggcggatg
     5641 cgatgctcat cacgaagctg gaaaacctgt ccatctacta catggatgac agccatcgcc
     5701 gcgtgattgt ggaaaacccg aaactcgacc gcgtggagaa ctacgagtca atgaacattg
     5761 attacgtggt ggaagactac gccgccggtt gtctggtgga aaaaattaag gtcggtgact
     5821 tctccacacc gactaaagtg accgcagagc cgggagcgta accgatgacg agtcccgcac
     5881 agcgccacat gatgcgggtc tcggcagcga tgaccgcgca gcgggacgcc gccccgctgc
     5941 gacatgcaac tgtctatgag cagatgctgg ttaagctcgc cgcagaccag cgcacactga
     6001 aagcgattta ttcaaaagag ctgaaggccg cgaaaaaacg cgaactgctg ccgttctggt
     6061 tgccgtgggt gaacggcgtg ctggagcagg gcaaaggtgc acaggatgac attctgatga
     6121 cggtcatgct gtggcgtctg gataccggcg atattgccgg tgcgctggag attgcccgtt
     6181 atgccctgaa gtacggtctg accatgccgg gtaaacaccg ccgcaccccg ccgtacatgt
     6241 tcaccgagga ggtggcgctc gcggccatgc gcgcccacgc tgccggtgag tctgtggata
     6301 cccgcctgct gacggagacc cttgcactga ccgccacggc agacatgcct gatgaagtgc
     6361 gcgcaaagct gcacaaaatc accggtctgt ttctgcgtga cgctggtgat gccgccggtg
     6421 cgctggctca cctgcaacgt gcgacacagc tcgactgtca ggcaggcgtc aaaaaagaga
     6481 ttgaacgact ggagcgggag ctgaaaccga agccggagcc gcagcccaaa gcggccaccc
     6541 gcgccccgcg taagacccgg agcgtgacac cggcaaaacg tggacgcccg aaaaagaaag
     6601 ccagttaaca accgaatgcg ccccgcgcca gggcggcacg ccggtcagtg agggtgaatc
     6661 acctgacact gcaccggcgt ccaccgcccg acttttcaga ggtagtcatg atgacgctga
     6721 ttattccgcg aaaggaggct cccgtgtccg gtgagggtac ggtggtcatc ccgcaaccgg
     6781 caggcgacga gccggtgatt aaaaacacgt tcttttttcc cgatatcgac ccgaagcgcg
     6841 tccgggaacg tatgcgcctt gagcagaccg tcgcccccgc ccgtctgcgt gaggccatca
     6901 agtcaggcat ggctgaaaca aatgcggagc tgtacgagta ccgcgaacag aaaattgccg
     6961 ccggttttac gcgtctggcg gacgttccgg cggacgacat cgacggtgaa agtatcaaag
     7021 ttttttacta cgagcgcgcc gtgtgtgcga tggcgaccgc gtcgctttat gagcgttatc
     7081 gcggcgtgga tgccagtgcg aaaggcgaca agaaggccga cagcattgac agcaccattg
     7141 atgagctgtg gcgggatatg cgctgggcag tggcgcgcat ccaggacaag ccgcgctgta
     7201 tcgtgagtca aatctgatga agacctttgc gctacagggc gacacgctcg acgccatctg
     7261 tgtccggtat tacgggcgca ctgagggtgt ggttgagacc gtgctcgccg caaatccggg
     7321 aatggctgaa ctgggtgcgg tgctgccgca cggcaccgcc gtcgaactgc ccgacgttca
     7381 gaccgcgccc gtggctgaaa ctgtcaatct gtgggagtaa cgcatgacag cagaagaaaa
     7441 aagcgtcctg tcgcttttca tgattggggt gctgattgtt gtcggcaagg tgcttgccgg
     7501 tggtgaaccc atcaccccgc gtctgtttat cgggcgcatg ttgctcggtg gttttgtctc
     7561 gatggttgcc ggtgttgttc tggtgcagtt tcctgacctg tcactgcctg cggtgtgcgg
     7621 tatcggctcc atgctgggta tcgccggtta tcaggtgatt gagattgcca ttcagcgccg
     7681 ctttaagggc agggggaaac cgtaatgccg gttattaaca cgcatcagaa tatcgccgcc
     7741 tttctcgaca tgctggccgt gtccgaaggg acggcaaacc atccgctgac gaaaaaccgg
     7801 ggctatgacg tgatagtcac cggactggac gggaagccgg aaattttcac cgactacagt
     7861 gaccacccgt tcgcacatgg ccgaccggcg aaggtgttta accgtcgcgg tgaaaaatcc
     7921 acggcctccg gtcgctatca gcagctttac ctgttctggc cgcattaccg caaacagctt
     7981 gccctgccgg atttcagtcc gttgtcacag gacagacttg ccattcagtt gatccgcgaa
     8041 cgcggtgcac tggatgacat ccgggcggga cgcattgagc gcgccatttc acgctgtcgc
     8101 aatatctggg cgtccctgcc gggagccggt tacggtcagc gtgagcattc actggaaaaa
     8161 ctggtcaccg tctggcgtac cgctggcggc gtaccggctt aaacggagta aacaccatga
     8221 agaaattatc cctttcactg atgctgaacg tgtcgctggc gctgatgctg gcactgtccc
     8281 tgatttaccc gcagagcgtg gccgtcagtt ttgtcgctgc ctgggcgatt ctggcgacgg
     8341 ttatctgtgt ggttgccggt ggtgtcggcg tgtatgccac agagtatgta ctggaacgct
     8401 acgggcggga gctgccgccg gaatcgctgg ccgtgaagat tgtcacgtcg ctgtttttgc
     8461 agccg
//
LOCUS       DACBVI010000173         8416 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.174, whole genome shotgun sequence.
ACCESSION   DACBVI010000173 DACBVI010000000
VERSION     DACBVI010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.174"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            110..2488
                     /gene="ppsA"
                     /locus_tag="IU325_19785"
     CDS             110..2488
                     /gene="ppsA"
                     /locus_tag="IU325_19785"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HAP5811245.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRSDMGLTNVEIMIPFVRTVDQAKAVVDELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2545..4245)
                     /locus_tag="IU325_19790"
     CDS             complement(2545..4245)
                     /locus_tag="IU325_19790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416216.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HAP5811246.1"
                     /translation="MHPTGPHLGPDVLSRESKMKVTLTFNEQRRAAYRQQGLWGDASL
                     ADYWQQTARAMPDKIAVVDNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPG
                     WCEFTVIYLACLKIGAVSVPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLIL
                     PLQNQLPQLQQLVGVDKLAPATSALSLSQIIADNTPLTTAITVHGDELAAVLFTSGTE
                     GLPKGVMLTHNNILASERAYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARS
                     VLLDIFTPDACLALLEQQRCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIP
                     KKVARECQQRGIKLLSVYGSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDAR
                     KTLPPGCEGEEASRGPNVFMGYFDEPELTARALDEEGWYYSGDLCCMDEAGYIKITGR
                     KKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLS
                     LEEVVAFFSRKRVAKYKYPEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE
                     "
     gene            complement(4248..4541)
                     /locus_tag="IU325_19795"
     CDS             complement(4248..4541)
                     /locus_tag="IU325_19795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAP5811247.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(4538..5827)
                     /locus_tag="IU325_19800"
     CDS             complement(4538..5827)
                     /locus_tag="IU325_19800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP5811248.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDVLEANVVILAD
                     GVNSMLGRSLGMVSASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRIVADIMNDMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(5883..6821)
                     /locus_tag="IU325_19805"
     CDS             complement(5883..6821)
                     /locus_tag="IU325_19805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HAP5811249.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPSMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTAH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(6841..7605)
                     /locus_tag="IU325_19810"
     CDS             complement(6841..7605)
                     /locus_tag="IU325_19810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HAP5811250.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQIATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGCGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINMPRIPSMKAILGAGKKPINQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEYLKKALN"
     gene            7921..>8416
                     /locus_tag="IU325_19815"
     CDS             7921..>8416
                     /locus_tag="IU325_19815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP5811251.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENF"
BASE COUNT         2013 a         2186 c         2176 g         2041 t
ORIGIN      
        1 tatatttgct tgaacgattc accgtttttt tcatccggtt aaatatgcaa agataaatgc
       61 gcagaaatgt gtttctcaaa ccgttcattt atcacaaaag gattgttcga tgtccaacaa
      121 tggctcgtca ccgctggtgc tttggtataa ccaactcggc atgaatgatg tagacagggt
      181 tgggggcaaa aatgcctccc tgggtgaaat gattactaac ctttccggaa tgggtgtttc
      241 cgttccgaat ggtttcgcca caaccgccga cgcgtttaac cagtttctgg accaaagcgg
      301 cgtaaaccag cgcatttatg aactgctgga taaaacggat attgacgatg ttacccagct
      361 tgcgaaagcg ggcgcgcaaa tccgccagtg gattatcgac actcccttcc agcctgagct
      421 ggaaaacgcc atccgcgaag cctatgcaca gctttccgcc gatgacgaaa acgcctcgtt
      481 tgcggtgcgt tcctccgcta ctgcagaaga tatgccggac gcttcttttg ccggtcagca
      541 ggaaactttc ctcaacgttc agggttttga cgccgttctc gtggcagtga aacatgtatt
      601 tgcttctctg tttaacgatc gcgccatctc ttatcgtgtg caccagggtt acgatcaccg
      661 tggcgtggcg ctctccgccg gtgttcagcg gatggtgcgc tccgacctcg catcttctgg
      721 cgtgatgttc tccattgata ccgaatccgg ctttgaccag gtggtgttta tcacttccgc
      781 atggggcctt ggtgaaatgg tcgtgcaggg tgcggttaac ccggatgagt tttacgtgca
      841 caaaccgaca ctggcggcga atcgcccggc tatcgtgcgc cgcaccatgg ggtcgaaaaa
      901 aatccgcatg gtctacgcgc cgacccagga gcacggcaag caggttaaaa tcgaagacgt
      961 accgcaggaa cagcgtgaca tcttctcgct gaccaacgaa gaagtgcagg aactggcaaa
     1021 acaggccgta caaattgaga aacactacgg tcgcccaatg gatattgagt gggcgaaaga
     1081 tggccacacc ggcaaactgt tcattgtgca ggcgcgcccg gaaaccgtgc gctcacgcgg
     1141 tcaggtcatg gagcgttata cgctgcattc acagggtaag attatcgccg aaggccgtgc
     1201 tatcggtcat cgcatcggcg cgggtccggt gaaagtcatc catgacatca gcgaaatgaa
     1261 ccgcatcgaa cctggcgacg tgctggttac tgacatgact gacccggact gggaaccgat
     1321 catgaagaag gcatctgcca tcgtcaccaa tcgtggcggt cgtacctgtc acgcggcgat
     1381 catcgctcgt gaactcggca ttccggcggt agtgggctgt ggtgatgcaa cagaacggat
     1441 gaaagacggt gagaacgtca ctgtttcttg tgccgaaggt gataccggtt atgtctatgc
     1501 ggagttgctg gaatttagcg tgaaaagctc cagcgtagaa acgatgcctg atctgccgtt
     1561 gaaggtgatg atgaacgtcg gtaacccgga ccgtgctttc gacttcgcct gcctgccgaa
     1621 cgaaggcgtg ggccttgcgc gtctggaatt tatcatcaac cgtatgattg gcgtccaccc
     1681 acgcgcactg cttgagtttg acgatcagga accgcagttg caaaacgaaa tccgcgagat
     1741 gatgaaaggt tttgattctc cgcgtgaatt ttacgttggg cgtctgactg aagggatcgc
     1801 gacgctgggt gccgcgtttt atccgaagcg cgtcattgtc cgtctctctg attttaaatc
     1861 gaacgaatat gccaacctgg tcggtggtga gcgttacgag ccagatgaag agaacccgat
     1921 gctcggcttc cgtggcgcgg gccgctatgt ttccgacagc ttccgcgact gctttgcgct
     1981 ggagtgtgaa gcagtgaaac gtgtgcgcag cgacatggga ctgaccaacg ttgagatcat
     2041 gatcccgttc gtgcgtaccg tagatcaggc gaaagcggtg gttgacgaat tggcgcgtca
     2101 ggggctgaaa cgtggtgaga acgggctgaa aatcatcatg atgtgtgaaa ttccgtccaa
     2161 cgccttgctg gcagagcagt tcctcgaata tttcgacggc ttctcaattg gctcaaacga
     2221 catgacacag ctggcgctcg gtctggaccg tgactccgga gtggtgtctg aactgttcga
     2281 tgagcgcaac gatgcggtga aagcactgct gtcgatggcg attcgtgccg cgaagaaaca
     2341 gggcaaatat gtcgggattt gcggtcaggg cccgtccgac cacgaagact ttgccgcatg
     2401 gttgatggaa gaagggatcg atagcctgtc tctgaacccg gataccgtgg tgcaaacctg
     2461 gttaagcctg gctgaactga agaaataaaa taaatccccg gcggcgttca gtcgccgggt
     2521 ttatgtgatc cccgaagatg aaacttattc aatctcttca cagacatcct gagttaatct
     2581 ccgcataata tctttcctta acaaaaactt ttgtatttta cctgaggcag tgcgcggtag
     2641 tttttcgatt accacgatat gttcaggata tttatatttc gcgacccgtt tacggctaaa
     2701 aaaagccacc acctcttcca gcgataatga atgatgcggt gctttcagca cgacataagc
     2761 gcatgagcgt tcacctaaac gttcatcggg catcgcaacc acacaggcat cgtgaatttt
     2821 aggatgctgc aataaaatat cttctacttc acggctgcta atattttcgc cgccgcggac
     2881 aataatatct ttcttgcgcc cggttatttt tatatagccg gcctcatcca tgcagcagag
     2941 atcgccgctg taataccagc cttcttcatc cagggcacgg gcggttaatt caggttcatc
     3001 aaaatacccc ataaacacat tggggccacg cgaggcttct tcaccttcgc aacctggcgg
     3061 taaggtcttg cgtgcgtcat cgaccacttt aatctcaaca cctgcggcag cgtaaccatc
     3121 ggtgtgcata aagcgcgaca aaggatcgtc gagattcacc actgcgtgcg gcgaactttc
     3181 tgtggaacca taaacactta ataatttaat gccgcgctgc tggcattcac gcgccacttt
     3241 tttggggatt gtggtcccgc cgcaaagaaa gaagcgcagc gctgaaaggt cagcgggttg
     3301 tttctccagt aaattcaaaa gatcatagac aaacggcgtt gcgccgagca tacaggtgca
     3361 acgctgctgc tcaagcagcg cgagacacgc atcaggagtg aaaatatcta acaacacgct
     3421 gcgagcgcca attaagaatg gtgccgttac gccatgcaga aagcccgttg catgaccaag
     3481 tggcgcaggc atcataaaga catcctgcca ggtcagattc aaccgcgcgc aataagcccg
     3541 ctcactggcg agaatattgt tatgcgttag catcacgccc tttggcagac cctcggttcc
     3601 ggaggtaaac agcaccgcgg ctaattcatc gccgtggacc gttatcgccg tggttagagg
     3661 agtattgtcg gcgataatct gacttaatga gagagcagag gtggcgggag ccagtttgtc
     3721 cacgccgaca agttgttgta gttgtggaag ctgattttgc agcggcagga ttaaatctac
     3781 cggacgcgtt tgtttaaaca acgtcggagc aaagaacatt tttgcctgac acttattgag
     3841 cacccatact agttctgctt cccgccagga aggcaacagc ggtacggaaa ccgcaccgat
     3901 tttcaggcag gcaagataga taacggtaaa ttcacaccag ccaggcagtt gaaatgcgat
     3961 gcgatcgcct gattcaatac ccttcgctaa catccagttt gccagacagc tcgcggcgtg
     4021 atcgagcgcg ctataggtgt acgatgctcc atgattatcg accacggcaa ttttgtctgg
     4081 catcgcacga gcggtctgct gccagtaatc ggccagcgaa gcatcgcccc atagcccttg
     4141 ctgacgatac gccgcacgac gttgttcgtt aaacgttaat gtcactttca ttttggactc
     4201 tcgagacaga acatcaggcc cgagatgcgg gcctgtggga tgcatgatta gccgtagcga
     4261 aaatcaatac cgaaggtgcc tgcgggatat tgccactgtt cgagaatagt gttgccgcac
     4321 agcacccgac aggtgccgca ctccagacaa ccggcagaat caaaatgaat gttgccggcg
     4381 tcatcctgct tataaagtcc ggcagggcag gctttcatta atttacggaa ttcattgata
     4441 tcgggatttg ccgccaaaat gatatgcggg tggccctcat caacatggaa tttattgacg
     4501 cctaatttga tgtcgacgtt aaccgtagcg ttctggctca tagcgcggtt gctcccttaa
     4561 tgccatcttt cagcaagttg atcagcccaa tttttttcgc gtgtcccatg atcattttgc
     4621 gtaccggctg gtttggtttg ccgtcaatgg tgaacatatc gttcatgatg tcggcgacaa
     4681 tgcgcggata ctgactaaac aggcgcggat tttccatcag cgccgggatc ttgcggaaat
     4741 gctgcatatc gcgcataaca caactttgtt ccagctcacg tttgtattgc gccagactgc
     4801 tggcggagaa atccgcgcgt tctttggcgg caatcactgt tgtggcggca gcctgagctg
     4861 atgcaatggc taaatccatg ccgcgaactg taaaacccaa attcaggcag aagcctgcgg
     4921 cgtcaccaac gatcatcacg ccatcgttaa ccagctgcgg caccattgcc agaccgcctt
     4981 ccggcaccat atgcgcggaa tattcaagca gtttgccgcc gctaatcagc gggcgaatgg
     5041 cggggtgttg tttaaaatct tccagcattt gcggcacgct tttttgcgca tgggcgatat
     5101 cacccaatcc acaaaccagc cccaacgata tggaatcctt attggtatag aggaatccgc
     5161 cacccatcag gccgtcagaa ggggaaccgg caaacagcca ggcggcacct tcctcgcccg
     5221 taatattaaa gcgatcgttg atctgttctg gtgtgaggcc aataacctct ttaacaccaa
     5281 cagcgtaatg atgcggatcg gaagcggaaa ccattcccag cgagcggcca agcatcgagt
     5341 taacgccatc agccagaatc accacattcg cttcaagaac atcatccccg gcctgcacgc
     5401 cagtgacctt gtttccttca cgaaccagcg catcgacgcg tacgcccggg ataaactgcg
     5461 cgcccgcctg ctcggcttgt tccatcaacc acgggtccag acgattacgc aatacggtat
     5521 aagacgcgtg ttgcggaaca tctggttgct cgcggtgaaa gtcgagggta acggcgcttt
     5581 cttcggttaa gaaggagatt ttctcgcgtg tgaccttgcg ttctaccggc gctgatgctg
     5641 caaagcctgg aataatcgct tcaagtgtgt gggcataaag acgcccgccg gtcatgtttt
     5701 tacatccggc actgtcgccg cgttctatca ccaggacatc cagcccggct cgcgccatga
     5761 ccagtgcggc aacgctaccg gcaacgcccg caccgaccac aatggcatca aatttgtcat
     5821 ccgacatgcg ttatgctccc ttcccgctgc gtttacagcg ggagatgtgg ttgaaagggg
     5881 aattagcggc tcaactggct aatcagggca gggacgactt tgtagatatc gcccaccaga
     5941 ccgtagtcgg catagttgaa gattggcgca tttttatctt tattgatggc gacaatcact
     6001 tttgcgccgt tgccgccaac catatgctgg atctgcccgg agatccccag cgtcaggtag
     6061 agatcggatt tcagcaacac gccggagaca ccgatataac gttcacgctc catccagttc
     6121 tcgccttcgg caattggacg tgaacagccg acttcagcat tcagcaccgc cgccagttcg
     6181 tggaccattt tcaggtcatc ctgcgctgcc agaccacgac cgacgccaac cacacgtttt
     6241 gctttgctca ggtccacgct gcttgccgct ttcgcacggc gttcgcgaca gagaatttca
     6301 tgacgcgggg caacataagg taccgtttct gtcgggcact gatgagcggt atcactggtg
     6361 cacggttcct gaacaccggg tgcaagggta atgatcgcca gcgggctgtt gatcttttcc
     6421 tgtgcgaacg ctaacccgcc atacatccgg tgttcggcgc aaatgtgacc atcgacaata
     6481 tccaccgcag tggcatcgtt caccagcgcc gcattcagtt gcacacttaa ccgtgctgcc
     6541 agagctttac cgcgtttcgt cgcggccagc agcaacatag aaggatgttt atctttcagc
     6601 agggcagcaa tgctttcggc gtaattttca gtacgttgca gcgcatcatt ttgctcaaga
     6661 acataaatac attttggacc ataaggcata actgcctgcg cctggtcggt attttgtaca
     6721 atggcataca cctgttggcc ccattgctgc gcgccgccaa acagttcagc ataacgttca
     6781 ggattatcgc taaagaccca gacgctgttt aattgactca tcgtttcttc tccgtaggct
     6841 tcagttcagg gctttcttca gatattccgc cagctcggca atggcctccg gcgaatcgtt
     6901 atcgagaatg atgtgcttac gttctgtttg cggcggtacg cgaatgccgg tcagctcagc
     6961 aagtggcgcg ctctggctcc agccaatgtc acttgcctgc cactgattta tcggtttttt
     7021 acccgcgccg agaatggctt tcatcgaagg aatgcgtggc atgttaatat cggaggtgac
     7081 gcagagcacg gctggaacag agagctcaat aacttcaaca tcatcttcaa gcgtgcgttc
     7141 aatcaccagt gtattgccct gacgctgaat agcactcact gcattaatca ccggaagttg
     7201 cagaatttct ccgaccagca agccaacctg ctgggcataa aggtcaccgc aaccttcacc
     7261 aaagatcagt aaatcgaagc cgatcttttc aactgctgcc gccagtgctt ttgcggtatc
     7321 gagaggcagt gcatgttcaa gttgcgcatc ctgcaccaaa tacaggctat gcggcccgcg
     7381 ggatagcacg tctttgcgta ctttcgagtt ctgcaacaat gagccgccaa cggtcagcgc
     7441 ggctatctca tcgtcatctg ttgcaatctg gcttgcagct tcaatggcat tgagatcgaa
     7501 ctggctgatt ttggcgtcgg cattgtcgaa attcagggta tattctggag tgacaacaat
     7561 gtcctgttct tcaggcacca gcttaaagca ggttattatt ttcatgattt ctcctgtgag
     7621 tcgcatacgg aaccatctcc gatggaaggt aaaaatggcc tgaaaatagt gtgcgatagc
     7681 ggtgcggggt tttcgacgga gcaagctgtt ttccttgcca acaatcttgc aaaaaaaatg
     7741 cgatcgctaa aacaaaagtg caatttgcgg caatatagtt attccatgac cgtacaatct
     7801 tgcattcagt gtaaataatc ttgtttagct ctcgctaaac gcggttgtat aaccgcaata
     7861 cacgccgtat tttatccggc atatattgtt tttgcttgct catggaatag ggaggttgtc
     7921 atgtatcaac gctgttttga taacgccagc gaaacgctgt ttgtcgccgg taaaacgcca
     7981 cggctttcac gttttgcatt tagcgatgat ccaaaatggg agtccggaca tcacgttcat
     8041 gacaatgaaa ccgagttgat ttacgtcaag aaaggggtag caaggtttac catcgattct
     8101 tcgttatatg tcgcgcacgc cgatgacatt gtggtgatag aacgtggcag gctgcatgcg
     8161 gtggcctctg acgttaacga tccggcaacg acgtgtacct gtgcgctgta cggctttcag
     8221 tttcaggggg ctgaggaaaa tcagctactg caaccgcatt cttgcccggt aattgccgca
     8281 gggcagggaa aagaagttat taaaacctta tttaatgagc taagtgtgat tttgccgcaa
     8341 agtaaaaata gccaaacatc ttcgttatgg gacgcatttg cctatacgtt agcaattctt
     8401 tactatgaaa acttta
//
LOCUS       DACBVI010000174         8399 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.175, whole genome shotgun sequence.
ACCESSION   DACBVI010000174 DACBVI010000000
VERSION     DACBVI010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.175"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..286
                     /locus_tag="IU325_19820"
     CDS             <1..286
                     /locus_tag="IU325_19820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811252.1"
                     /translation="NTNELTRNDNIFILFSSVYDGPVMVVSSQRLHDMLNPTKDTNWN
                     STYIYKSRHEMLPVNLTPETLFGTKSQDKHALFPIFTASWRANRIKNKGI"
     gene            509..1006
                     /gene="ubiC"
                     /locus_tag="IU325_19825"
     CDS             509..1006
                     /gene="ubiC"
                     /locus_tag="IU325_19825"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HAP5811253.1"
                     /translation="MSHPALTQLRALRYFTEIPALDTQLRDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1019..1891
                     /locus_tag="IU325_19830"
     CDS             1019..1891
                     /locus_tag="IU325_19830"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HAP5811254.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2046..4469)
                     /gene="plsB"
                     /locus_tag="IU325_19835"
     CDS             complement(2046..4469)
                     /gene="plsB"
                     /locus_tag="IU325_19835"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HAP5811255.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4640..5008
                     /locus_tag="IU325_19840"
     CDS             4640..5008
                     /locus_tag="IU325_19840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="catalyzes the phosphorylation of diacylglycerol to
                     form diacylglycerol phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP5811256.1"
                     /translation="MANNTTGLTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5118..5726
                     /gene="lexA"
                     /locus_tag="IU325_19845"
     CDS             5118..5726
                     /gene="lexA"
                     /locus_tag="IU325_19845"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HAP5811257.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5745..7124
                     /gene="dinF"
                     /locus_tag="IU325_19850"
     CDS             5745..7124
                     /gene="dinF"
                     /locus_tag="IU325_19850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709876.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HAP5811258.1"
                     /translation="MPPGVAVCFSSLFIRLVCMAFLTSSDKALWHLALPMIFSNITVP
                     LLGLVDTAVIGHLDSPVYLGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQA
                     LARALVQPLLLALGAGALIALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPAS
                     LANLVLLGWLLGVQYARAPVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYA
                     TLLIGLLMVRKILKLRGISGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVL
                     GARLGSDIIAVNAVLMTLLTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACR
                     QSGIVALLFSVVYLLAGEHIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMF
                     IGATRAAEMRNSMAVAAAGFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHW
                     RNGTWFAAT"
     gene            7240..7449
                     /locus_tag="IU325_19855"
     CDS             7240..7449
                     /locus_tag="IU325_19855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP5811259.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7491..8006)
                     /gene="zur"
                     /locus_tag="IU325_19860"
     CDS             complement(7491..8006)
                     /gene="zur"
                     /locus_tag="IU325_19860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HAP5811260.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKSR"
BASE COUNT         1929 a         2037 c         2281 g         2152 t
ORIGIN      
        1 taacacgaat gaattaacac gaaacgataa tatctttatc ctgttctcct ctgtctatga
       61 tgggccagtt atggtggtaa gctcccagcg cttacatgat atgttgaatc ctacaaaaga
      121 taccaattgg aattccacgt atatctacaa atccagacat gagatgttgc ctgttaatct
      181 tactccggaa acacttttcg gcaccaaatc tcaggataaa catgctcttt tcccaatttt
      241 tactgcgagt tggcgagcta atcgtataaa gaataaaggt atttaagtaa aggaaaacat
      301 catcgttcct ggcatcctgg gcggtgatgt ccctatggtt gccctcgcca gcacgggcat
      361 cggcaaagcg taaggttcaa cagcgtttta ccacttcatg cgattgttgc gtttttgttg
      421 cgcattagat cacttaattt gctttacatc tcccgtaaac acttttctgc gatacaatgc
      481 ctttacgtta tgtaacggag agttcggcat gtcacacccc gcgttaacgc aactgcgtgc
      541 gctgcgctat tttacagaga tccctgccct ggatacgcaa ctgcgcgact ggctgttgct
      601 ggaagattcc atgacaaaac gttttgaaca gcagggaaaa acggtgagcg tgacgatgat
      661 ccgcgaaggg tttgtcgagc agaatgaaat ccccgaagaa ctgccgctgc tgccgaaaga
      721 gtctcgttac tggttacgtg aaattttgtt atgtgccgat ggtgaaccgt ggcttgccgg
      781 tcgtaccgtc gttcctgtgt caacgttaag cgggccggag ctggcgttac aaaaattggg
      841 taaaacgccg ttaggacgct atctgttcac atcatcgaca ttaacccggg actttattga
      901 gataggccgt gatgccgggc tgtgggggcg acgttcccgc ctgcgattaa gcggtaaacc
      961 gctgttgcta acagaactgt ttttaccggc gtcaccgttg tactaagagg aaaaaaatat
     1021 ggagtggagt ctgacgcaga ataagctgct ggcgtttcat cgcttaatgc gtacggataa
     1081 gccaattggc gcgttactgc tgctctggcc aacattatgg gcgttgtggg tggcgacacc
     1141 gggcgttccc cagctctgga tcctggcggt gtttgtcgcg ggtgtctggc tgatgcgcgc
     1201 tgccggatgt gtggtgaatg attatgctga ccgcaagttt gatggtcatg ttaagcgcac
     1261 ggcgaaccga ccgcttccca gcggcgcggt aacggagaaa gaggcgcgcg cgctgtttgt
     1321 cgtgctggta ctgatttcgt ttttactggt gctgacgctg aatacgatga ccattctgtt
     1381 gtcgattgcc gcgctggcgc tggcgtgggt gtacccgttt atgaagcggt atacccatct
     1441 accgcaagtg gtgctgggcg cggcgtttgg ctggtcgatt ccaatggctt ttgctgctgt
     1501 gagtgagtcg gtgccattga gttgctggtt aatgttcctc gccaatattc tctgggcggt
     1561 ggcttacgac acgcagtatg cgatggttga ccgcgatgat gatgtgaaga ttggcattaa
     1621 atccaccgca atcctgttcg gccaatacga taaattgatt attggtattt tgcagattgg
     1681 cgtactggcg ctgatggcga tcatcggtga gttaaatggc ttaggctggg gatattactg
     1741 gtcaattctg gtggctggcg cgctgtttgt ttatcaacaa aaactgattg ccaaccgcga
     1801 gcgtgaagcc tgctttaaag catttatgaa taataactat gttggtctgg tactgttttt
     1861 agggctggca atgagttact ggcatttctg atgatgtaaa aaagccggat gatcatccgg
     1921 cttccttctg ggttgcctga tgcgctgcgc ttctcaggcc tacacaacat atcgcaattt
     1981 attgaatttg cagatcatgg taggccgaat aaggcgtttg cgccgcatcc ggcaattctc
     2041 cctgattacc cttcgccctg cgtcgcactc tcaatcgtca aacgcacgtc tgatgtaatc
     2101 aactccgcca gcaactgata caccttcatc gtttctgccg gttcggcgtc gccgctatcg
     2161 ctgatatacc cttcatcacg cagtgttagc accagagaac tgaacaccgc cttgtcgaag
     2221 aactccggcg cgttgatacc gtgcagcacg gagagacgtt gcgcgacggt gcggctctct
     2281 ttctccagcg taccgcggtt gatcgacggg ttggcgctca acagccagaa ggtaatggca
     2341 taacgttgca gcgtttcgcg cgcgcctgcg gccagcagct gtagcgtgcg agaatgcgcc
     2401 gggttgatat gcaactcatc atcttgcagg gtaatcagcc cctgacgttg catctcattt
     2461 gccagtgcat caataacgtc cggcaactcg tcgcgatccc agcgcaggaa cagctccgct
     2521 ttcagcattg ggtaaagcac attgacgtgc tccatcaata cgtcgcggga gatgtggcga
     2581 tgctgggtga caattgccgc catcagcgaa ggcagcacca acatatgcgc aatgttgttg
     2641 cgatagtagg tcatcagcac cgcttgctcg cgcggcagaa tgatgatgtc gccgatagtg
     2701 tctttctcaa cttcaaactt gttcatttgc agcgcgtgat cgataagctc gctggcgctg
     2761 gctgaaggaa cggtagagtc cgtggagtaa ggcacgttgc gcatcagatc caggtagcag
     2821 ttgagttgct cggttaactg ctcgcgggtg agtgagcgct gacgtgacgc cagaagcgca
     2881 gtacagcaca ggttcatggc gtttgccgcg cctgcgttgt taatgcgtac catcagatcg
     2941 gcagcaatat tattgaccgt cggcgttaac caggccggac gcaccgcttc gatgggatcg
     3001 atagattcac gccagtctgg tacgtgctgg ttaaggtagg tcatcaacgg cattggttca
     3061 ccgaagttga cgtaaccctg accgagatta cgcagcttgc ttaaaccgcg cagcatctgc
     3121 ggcaggctct ctttctcttt cgttgcaccg cgcagttctt tggcgtaagt acccacttcc
     3181 atgacgtgct cataaccgat gtagatcgga atcagcgtaa tcggacgcgt gccgccacgc
     3241 agcatcgcct gaatggtcat cgatagcgtt ccggttttcg gatccagcaa acgccccgta
     3301 cgggaacgac cgccttccac gaaatactcg acggaataac cacggctgaa cagttcgccg
     3361 agatactcac ggaaaacggt ggaatagagc ttattgcctt taaacgtacg gcgaataaag
     3421 aacgccccca gacggcggaa aatcggcccg gcaggccaga aattcaggtt gatcccagcg
     3481 gcgatatgcg gcggcaccag cccctggtga tacagcacgt aagaaagcag caggtagtcc
     3541 atgtgactgc ggtggcaagg cacatacacc agctcatgac cgtcgtgggc caactggcga
     3601 acgcgctcgg cgttatggac gttgataccc tggtaaagtc ggttccaggt gaagcccaga
     3661 atacggtcag tcaggcgaat catctcgtaa gagaaattcg ccgcaatctc ttccatcagt
     3721 gcaatcgcgt tctgctgcgc tttttcatgg gagatttttt tgctgcgcgc ttcatcttct
     3781 accgctttcg caatggcgcg ggaggcgagc agcttattaa acagatcctg acgagcaggc
     3841 aaacgtgggc ctacggcagc cagacgttga cgggcaaagt gcatacgcgc cacgcgcgcc
     3901 agtttctgcg cgatagtttt atccgtgccg tgttcatccg ccatacggcg cagcgaaact
     3961 gacggtgaga aacgcacaaa actgtcgcga ccgagccaca gtacagcgaa aaatttctgt
     4021 acgccgttaa gcatacgcag cggcgggttc acttcgcctt tttcacgccc cggcgcgcga
     4081 ccaaacatca cagacactgg caccatctgc acatccagat ttgggttgct acggtgcaaa
     4141 tcgagatagt cgtggaacag cttaatagac tcttctttcg gcgtgtaata ggtgaataca
     4201 cgcggcccgc cgtgaatgaa cacatagcgc ggcagtagcg tgccgtcgat ttccagcggc
     4261 tctaacgggt caggcaggtc atgtgccaga cactgggcgc gcaacgtcag caagtctgct
     4321 ttcgagttgt acggtaaaac gtacataatt ggacgagagg tatccagccc cagttccggg
     4381 gcaggatctg ccggaataga cttgcttttt accaggatgc ttaatggtaa attcagtaat
     4441 ttgtagtaaa ttcgtggcca gccggacata aacgatgtaa agcctctggt taataatgca
     4501 aatgcgcggc aaggatagca gaaagtcatg ggaaattctg tggtatccgc tcatgtttcg
     4561 cgcggcgtta cgcaaacccg aatcatcgga tttaacggta cactgatatt gacgctcata
     4621 atgtaaaaag gttctttcaa tggccaataa taccactgga ctcacccgaa ttatcaaagc
     4681 tgctggctat tcctggaaag gtttacgcgc tgcatggatc aacgaagcgg cattccgtca
     4741 ggaaggcgta gcggtattgt tggcggtggt catcgcctgc tggctggatg tggacgcaat
     4801 tacccgcgtg ctgcttatca gctccgtgat gctggtgatg attgtggaaa tcctcaatag
     4861 cgccatcgaa gcagtggttg accgaattgg ctctgaatac catgagctat ccggacgcgc
     4921 taaagatatg ggatccgccg cggtgctgat tgcgattatc gtcgccgtga ttacctggtg
     4981 catcctgtta tggtcgcatt ttggataacc cttccagatt tcgataaatc tctggtttat
     5041 tgtgcagttt atggttccaa aatcgccttt tgctgtatat actcacagca taactgtata
     5101 tacacccagg gggcggaatg aaagcgttaa cggccaggca acaagaggtg tttgatctca
     5161 tccgtgatca catcagccag acaggtatgc cgccgacgcg tgcggaaatc gcgcagcgtt
     5221 tggggttccg ttccccaaac gcggctgaag aacatctgaa ggcgctggca cgcaaaggcg
     5281 ttattgaaat tgtttccggc gcatcacgcg ggattcgtct gttgcaggaa gaggaagaag
     5341 ggttgccgct ggtaggtcgt gtggctgccg gtgaaccgct tctggcgcaa cagcatattg
     5401 aaggtcatta tcaggtcgat ccttccttat tcaagccgaa tgctgatttc ctgctgcgcg
     5461 tcagcgggat gtcgatgaaa gatatcggca ttatggatgg cgacttgctg gcagtgcata
     5521 aaactcagga tgtacgtaac ggtcaggtcg ttgtcgcacg tattgatgac gaagttaccg
     5581 ttaagcgcct gaaaaaacag ggcaataaag tcgaactgtt gccagaaaat agcgagttta
     5641 aaccaattgt cgttgacctt cgtcagcaga gcttcaccat tgaagggctg gcggttgggg
     5701 ttattcgcaa cggcgactgg ctgtaacata tctctgagac cgcgatgccg ccaggcgtcg
     5761 cggtttgttt ttcatctctc ttcatcaggc ttgtctgcat ggcattcctc acttcatctg
     5821 ataaagcact ctggcatctc gccttaccca tgattttctc caatatcacc gttccgttgc
     5881 tgggactggt cgatacggcg gtaattggtc atcttgatag cccggtttat ttgggcggcg
     5941 tggcggttgg tgcaacggcg accagctttc tctttatgct gttgctgttt ttgcgcatga
     6001 gcactaccgg gctgactgcg caggcttatg gtgccaaaaa tcctcaggca ttagcccgtg
     6061 cgctggtgca accgttgctg ttggcgttgg gggccggggc gttaattgcg ctgctgcgta
     6121 cgccgattat cgatctggcg ctgcatattg ttggcggcag cgaagcggtg ctagaacagg
     6181 cgcggcgctt tcttgaaatc cgctggttaa gcgcaccggc gtcgctggcg aatctggtat
     6241 tacttggttg gttactcggc gtgcaatatg cccgtgcacc agtaattttg ttagtggtcg
     6301 gcaatatcct caacattgtg ctggatgtct ggctggtgat ggggctgcat atgaacgtgc
     6361 agggcgcggc gctggcgacg gttattgcgg aatatgcaac attgctgatt ggtctgctaa
     6421 tggtgcgtaa aatcctcaaa cttcgcggaa tatccggcga aatgctgaaa actgcctggc
     6481 gaggaaactt ccgtcgcttg ctggcgctta accgcgatat catgctgcgc tcgctgttgt
     6541 tgcaactctg tttcggcgcg atcaccgtac ttggcgcgcg actggggagt gacattatcg
     6601 ctgttaacgc ggttctgatg acgttactca cctttaccgc ctatgcgctg gatggttttg
     6661 cctacgcggt tgaagcacat tctggtcagg cgtacggtgc gcgcgacggt agccagttac
     6721 tggatgtctg gcgggcagcg tgccgccagt cggggattgt agcgttactg ttttcggtgg
     6781 tttatttgct ggcaggggaa cacatcattg cgttactgac gtcgttaacc cagattcagc
     6841 agctggccga ccgctatctt atctggcagg tgattttgcc gttggttggc gtctggtgtt
     6901 atctgctgga cggcatgttt ataggcgcaa cgcgtgccgc cgaaatgcgt aacagtatgg
     6961 cggtggccgc cgcaggtttt gcgctgacgc tccttacgct gccgtggctg ggtaatcatg
     7021 gtttgtggct ggcattaacc gtctttctgg ctttgcgcgg gctttctctg gcggctatct
     7081 ggcggcgtca ctggcgcaat ggtacctggt ttgccgcaac gtgacggtta aaaattctga
     7141 ataaataatc ctaagccaaa ttgctgacta cacttaatct cacgttcaga agaaaagtga
     7201 acgtactctc attcacaacc taacgatgag gtcttgatta tgaataaaga tgaagccggc
     7261 ggtaactgga aacagtttaa aggtaaagtg aaagagcaat ggggcaaact gaccgatgat
     7321 gatatgacga tcattgaagg taaacgtgat caactggtcg gtaaaatcca ggaacgttat
     7381 ggttatcaga aagatcaggc agaaaaagag gtcgtggatt gggaaacccg caatgagtat
     7441 cgctggtaat taatccctcc tgcccgacgt gtacaaggat gtacgccctc ttaacgtgat
     7501 ttctttttca cctgtacaga gtgatcatgc tggcactgtt caggatgacg acacgcttcc
     7561 acttccacgc atgccgcaca gagtccatgt gcttcaatca cattatgccg cagggcaaac
     7621 cccattttgg ccgccagcgt atgcataatg tcttccacgc cttctgcaca ttcttctttc
     7681 accgcgccgc agcgatcgca aataaacatg gctgaagtat gggtgggctg gtcgaacaga
     7741 tgacagagca catagctgtt ggtggattcc accttatgca caaaaccttg ctcaagaaga
     7801 aaatccagcg cgcgataaac cgttggcggc ttggcttgcg gctcagcttc gcgcagcaaa
     7861 tcaagcagat cataagcgct gatagcgcca tcctgcagac tcatcaggcg caacacttcc
     7921 aggcgctgtg gggtcaggcg cacattacgc tgcgcgcaga ttttttcagc ctgcgctaat
     7981 aactcctgcg ttgtggtctt ttccatcggg cacctcaaag tgggggttaa gaatgtcctt
     8041 actttaccat gttccaggaa aaacaccgag aacccttatt gttgccgtaa tgttgatttt
     8101 ctgttttgta cgtaaggtgt tgttttgcct ggtagtacca ttatcaacac gataataatt
     8161 aataattcat ttttaaataa attgcttcaa gttaaatcgc tatttattta gctgggtttg
     8221 attgcatacc tgtacttcag agattatgag cgatttttga tagtgagcag atggcaaaag
     8281 aattataagg aggacacggt acgacaaaaa aggtagatga atcatgttga agataatacc
     8341 cggtgcaact ggatacttta ataaaacact caattcaaat caattcgata atgaagatg
//
LOCUS       DACBVI010000175         8366 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.176, whole genome shotgun sequence.
ACCESSION   DACBVI010000175 DACBVI010000000
VERSION     DACBVI010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.176"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..143
                     /locus_tag="IU325_19865"
     CDS             <1..143
                     /locus_tag="IU325_19865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HAP5811261.1"
                     /translation="FSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSG
                     LS"
     gene            414..2651
                     /gene="nrfB"
                     /locus_tag="IU325_19870"
     CDS             414..2651
                     /gene="nrfB"
                     /locus_tag="IU325_19870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HAP5811262.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMINRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            2638..5610
                     /locus_tag="IU325_19875"
     CDS             2638..5610
                     /locus_tag="IU325_19875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage receptor"
                     /protein_id="HAP5811263.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDARARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKTRLQHYLIEN
                     TPLFTTDAQEKSWLYLLSKYSGNPVQALANYTVQFADNRQYVVVATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYTVSVATHNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLESEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5611..6501
                     /locus_tag="IU325_19880"
     CDS             5611..6501
                     /locus_tag="IU325_19880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HAP5811264.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVNKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPNAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            6684..7436
                     /locus_tag="IU325_19885"
     CDS             6684..7436
                     /locus_tag="IU325_19885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP5811265.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQK"
     gene            complement(7490..7566)
                     /locus_tag="IU325_19890"
     tRNA            complement(7490..7566)
                     /locus_tag="IU325_19890"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7530..7532),aa:Arg,seq:tct)
     gene            7808..>8366
                     /gene="fimZ"
                     /locus_tag="IU325_19895"
     CDS             7808..>8366
                     /gene="fimZ"
                     /locus_tag="IU325_19895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HAP5811266.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNNELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKS"
BASE COUNT         2098 a         2149 c         2122 g         1997 t
ORIGIN      
        1 tattttcagg gatacggcac atcgctatta atattttgac gaatgataag gtattcaagg
       61 cagggttaag acgtaagatg cgaaaagcag ccatggacag aaactatctg gcgtcagtcc
      121 ttgcggggag cgggctttcg taatcttgcc ctgcctgctc acctcctcca ggcacttcca
      181 ctacatgatg acctgctcgg tgccaccgct ccttttcact atgacgttaa cctccctgtc
      241 ccaggacacc atgcttttgt ctacattcta cgcaaacggc aacttttata accagtataa
      301 aaataacgtg ccgcaatagt catacctatt aatggtaaaa agctgtcaca attcataaaa
      361 aaccttaata tacgccaccc taaacataac cagcgttaat gtaaggtttt tgtgtggact
      421 ggcttcttga tgtttttgct acctggctct acggcttaaa agtaatcgcg ataacgttag
      481 cggtcatcat gttcatcagc gggctggacg atttttttat tgatgtcgtc tactgggtac
      541 gccgcattaa acgcaagttg agtgtttatc gccgctaccc gcgaatgagt taccgcgaac
      601 tgtataaacc agatgaaaaa ccgttagcga ttatggttcc ggcgtggaat gaaacgggcg
      661 tcatcggcaa tatggccgag ctggcggcga ccacgctcga ctatgaaaac tatcatatct
      721 ttgttggcac ctaccccaac gaccccgata ctcagcgtga tgttgacgaa gtgtgcgctc
      781 gcttcccgaa tgtgcataag gtagtctgcg cgcgacctgg ccccaccagc aaagccgact
      841 gcctgaacaa cgtgctggac gccatcaccc agtttgagcg tagcgccaat ttcgcttttg
      901 ctggttttat tctgcatgac gccgaagatg tgatttcacc gatggaattg cgtctgttca
      961 actatctggt cgagcgtaaa gatctgattc agatcccggt gtatccgttc gaacgcgaat
     1021 ggacgcactt caccagcatg acttacattg atgagttttc agaactgcat ggcaaagatg
     1081 ttccggtgcg tgaagccctc gccggacaag tgcccagcgc aggcgtcggt acctgtttca
     1141 gccgccgcgc cgtgaccgca ctgttagctg acggtgacgg tattgctttc gacgtgcaga
     1201 gtcttactga agattacgac attggcttcc gcctgaaaga aaaaggtatg acggaaattt
     1261 ttgtccgttt tccggtggtg gacgaagcca aagaacgcga gcagcgtaaa tttttacagc
     1321 acgcgcggac gtcaaacatg atctgcgtgc gcgaatattt ccccgatacc ttttcgactg
     1381 cggttcgaca aaaatctcgc tggatcatcg gcattgtttt ccagggcttt aaaacccaca
     1441 aatggacctc cagtctgacg ctgaactact ttctctggcg cgaccgcaaa ggggcaatca
     1501 gtaactttgt cagcttcctc gcgatgctgg tgatgatcca gcttttgctg ttgctggcgt
     1561 atgaaagttt gtggcccgat gcctggcatt tcctttctat tttcagcggc agcgcatggt
     1621 taatgactct gctgtggcta aactttggct tgatgattaa ccgcatcgtg cagcgggtga
     1681 ttttcgttac tggctactac ggcctgacgc aggggctgct atctgtcctg cgtcttttct
     1741 ggggcaacct gattaacttc atggccaact ggcgcgcgtt aaaacaggta cttcaacacg
     1801 gcgatccacg tcgtgtcgcg tgggataaaa caacgcatga cttccccagc gtgactggcg
     1861 atacccgctc gttgcgtccg ttaggtcaaa tcctgctgga aaatcaggtc atcactgaag
     1921 aacaactcga tacagcactg cgtaatcgcg tcgaaggtct acgcctgggc ggttcaatgc
     1981 tgatgcaggg gctgattagc gccgaacagc tggcacaggc gctggcagag caaaacggcg
     2041 tggcgtggga gtcgatcgac gcctggcaga tcccttcatc actgattgcc gaaatgccgg
     2101 cttccgtggc gctgcattat gcagtactgc cgctgcgtct ggaaaatgat gagttaattg
     2161 tcggcagtga agatggtatt gacccggttt cgctggcggc cctgacgcgt aaagtcggac
     2221 gcaaagtgcg ttacgtcatt gttctgcggg gacaaattgt cacggggtta cgccactggt
     2281 atgcgcgccg acgcggtcac gatccgcggg caatgttgta caatgcggtt cagcatcagt
     2341 ggctcacgga acagcaggcc ggtgaaatct ggcggcaata tgtgccgcat cagttcctgt
     2401 tcgccgaaat actgaccacg ctcggtcata ttaatcgttc agcaattaac gtgttgttat
     2461 tgcgccatga acgcagttct ctgccgctcg gcaagttttt ggtcaccgaa ggcgttatca
     2521 gccaggaaac gttggatcgc gtcctgacaa ttcaacgcga attacaagtt tcgatgcaat
     2581 cactattact caaagcaggt ttaaacacag aacaggttgc gcaactggag tccgaaaatg
     2641 aaggagaata accttaatcg cgtcatcgga tggtctggtt tactgctgac gtctttattg
     2701 agtaccagcg cactcgcaga caatatcggc accagcgcag aagagctggg gctgagcgat
     2761 tatcgccatt ttgttattta tccccgtctc gacaaggcgc tgaaggcaca gaaaaataac
     2821 gacgaagcaa ccgccatccg cgaatttgaa tatatacacc agcaggtgcc ggataatatt
     2881 ccgctaactt tataccttgc tgaagcctat cgccattttg gtcatgatgc ccgggcgcgg
     2941 ctgttgcttg aggatcaact gaaacgtcac ccaggagatg cccgacttga gcgcagtctg
     3001 gcggctattc cagttgaagt gaaaagcgtt acgactgttg aagaactgct tgcccagcaa
     3061 aaagcgtgcg atgctgcgcc gaccctgcgt tgtcgcagtg aagtcgggca gaatgccctg
     3121 cggctggcac aattacctgt cgccagagcg caactgaacg atgcgacgtt tgctgcatcg
     3181 ccggaaggaa aaacgctgcg aaccgatctg ctgcaacgcg caatctacct gaaacaatgg
     3241 tcccaggcag atacgctata caatgaagca cgccagcaga acacattaag cgcggcagaa
     3301 cgccgtcagt ggtttgacgt gcttcttgcc gggcagctgg atgatcggat cctggcgctg
     3361 caatcacagg ggatcttcac cgatcctcag tcatatatta cttacgcgac cgcgctggct
     3421 tatcgtggcg aaaaaacacg cctccagcat tatctcattg aaaatacgcc gctatttacc
     3481 acggacgcac aagagaaaag ttggctctat ctgttatcta aatacagcgg caaccccgtt
     3541 caggcgttgg cgaattatac ggtacagttt gccgataacc gccagtatgt tgttgtcgcg
     3601 acgctaccgg tgctgttaaa agaaggtcag tacgacgcag cgcaaaaact gctcgccacc
     3661 ctccccgcca atgaaatgct tgaggagcgt tataccgtta gcgtagcgac ccataacaag
     3721 gctgaagctc tgcgtctggc acgattgctg tatcagcaag aaccggcaaa tcttacccgc
     3781 ctggatcaac tgacctggca actgatgcag aacgagcagt cacgcgaagc tgccgattta
     3841 ttgctgcaac gctatccttt ccagggcgat gcgcgtgtca gccagacttt aatggcgcga
     3901 ctggcgtctc tgctggaaag tcatccttac ctggcaacgc cggcgaaggt ggcgatttta
     3961 tcgaaaccct taccgctggc ggagcaacgt cagtggcaaa gtcagttgcc gggtattgca
     4021 gataattgcc cggcaatagt tcgcttgctg ggcgatatgt cgccttccta cgatgccgcc
     4081 gcctggaacc gtctggcaaa gtgttatcgg gacacgctac ccggtgtggc gttgtatgca
     4141 tggcttcagg ccgaacaacg acaaccgaac gcctggcaac atcgtgcggt agcctatcag
     4201 gcgtatcagg ttgaggacta cgccaccgca ctggcggcct ggcagaaaat cagtcttcac
     4261 gacatgagca atgaggatct gcttgctgct gccaataccg cccaggcggc aggaaatggt
     4321 gcggctcgcg atcgctggct tcagcaggca gaacaacgtg ggctgggaaa caatgccctc
     4381 tactggtggc tacatgcgca acgttacatt cctggtcagc cggaactcgc actgaacgat
     4441 ctcacgcgct caatcaatat tgcgccttct gccaacgctt acgttgcgcg ggcgacaatt
     4501 tatcgccaac gtcataatgt cccggcggcg gtgagtgatt tgcgcgccgc gctggaactg
     4561 gaaccgaata atagcaacac ccaggcagcg ctcggttacg ccttgtggga tagcggtgat
     4621 atcgcacagt cgcgggaaat gctcgaacag gcgcataaag ggctaccgga cgatccggca
     4681 cttatccgac aactggccta cgtgaaccag cgtctggatg acatgcctgc gacgcagcac
     4741 tacgcccggc tggtgattga tgacattgat aatcaggcgc tgataacccc actgacccca
     4801 gagcaaaatc agcaacgctt caatttccgc cgtctgcatg aggaggtcgg tcgccgctgg
     4861 acgttcagtt tcgattcttc catcggcttg cgttccggcg cgatgagtac cgctaacaat
     4921 aatgtcggcg gcgcagcgcc agggaaaagc tatcgtagct acgggcaact ggaatccgag
     4981 taccgcatcg gacgcaatat gctgctggaa ggcgacctgc tctcagttta tagccgcgtc
     5041 tttgccgata ccggagaaaa cggggtgatg atgccggtga aaaatccgat gtccggcacc
     5101 ggtctgcgct ggaagccgct gcgcgatcag atctttttcc tcgccgtcga acagcagttg
     5161 ccgctgaacg ggcaaaatgg cgcatccgat accatgctgc gcgccagcgc ctcattcttt
     5221 aatggcggca aatacagcga cgaatggcac ccgaacggtt caggctggtt tgcccaaaac
     5281 ctgtacctcg atgcggcgca atatatccgc caggatattc aggcgtggac ggcagattat
     5341 cgcgtaagct ggcatcagaa ggtagctaac ggacagacta ttgagcctta cgctcacgtt
     5401 caggacaacg gctatcgtga taaaggcact cagggcgcgc agcttggcgg tgtcggggtc
     5461 cgctggaata tctggaccgg cgagacgcac tacgacgcct ggccgcacaa agtcagtctc
     5521 ggcgtcgaat atcaacatac ctttaaggcg attaatcaac gtaacggaga gcgcaacaac
     5581 gcgtttctca ccattggagt gcactggtaa atgcgtaagt tcattttcgt attgctgaca
     5641 ctgcttttgg tcagcccttt ttcctttgcg atgaaaggta ttatctggca accacaaaac
     5701 cgtgatagtc aggttaccga tacccagtgg caggggctga tgagtcagtt acgtttgcaa
     5761 ggcttcgata ccctggtttt gcaatggacc cgttacggcg atgcattcac ccagccagaa
     5821 cagcgcgcgt tattgtttaa gcgtgcagca gctgcgcaac aggctggcct gaagctgatt
     5881 gtcgggctga atgccgatcc ggaatttttt atgcaccaga aacagtcatc cgcggcgctg
     5941 gaaagctatc ttaatcgcct gctggctgcc gatctccagc aagccagatt atggagcgcc
     6001 gcgcctggcg taacgccgga tggctggtat atcagcgcgg aaattgacga cctgaactgg
     6061 cgcagcgaag ccgcccgtca acctttgctg acatggttaa acaacgctca gcggttgatt
     6121 agcgatgttt cagcaaaacc ggtttatatc agtagttttt tcgccggaaa catgtcgccc
     6181 gatggctatc gccaactgct ggaacacgtt aaagcaaccg gcgttaatgt ctgggtacag
     6241 gatggcagcg gcgtgaataa acttaccgct gaacagcgtg aacgttatct acaggccagc
     6301 gccgattgcc agagttccgc cccagcaagc ggcattgttt atgaactgtt tgtcgccggc
     6361 aaaggcaaaa cctttacagc gaaaccgaaa ccgaatgcag agatcgcctc gctgttagcg
     6421 aaacgttcct cttgcggtaa agacacgctc tatttctctc tgcgctattt gcccgtcgca
     6481 cacggcattc tcgagtatta aatcccctcc aggtaagtcg ggtacgacct ggcttacctc
     6541 tttcgctctc gtcagaattc catcaagatt aattgctaaa aagcgtgcta aagaaagaag
     6601 atcgttatcg gcgaacgggc cgatcactca tgcaggaagc atagatcctt tgagcggagt
     6661 ttctgatatc tgaggagtgc gaaatgcaat tgagcagcag tgaaccttgc gtggtgatcc
     6721 tgaccgaaaa agaggtagag gtaagcgtta ataaccatgc cacgtttacc cttccgaaaa
     6781 attacctggc cgccttcgcg tgcaacaata acgtcattga actctcaacg ttaaatcacg
     6841 tattaatcac ccacatcaac cgcaacatca tcaacgatta tctgttgttt ttaaataaga
     6901 acttaacctg cgtaaagccc tggtcgcggc tggctacccc ggttatcgct tgtcatagcc
     6961 gtacaccgga agtgttccgg ctagccgcca accacagcaa gcagcaaccc agcaaaccct
     7021 gcgaagcgga gttgacgcgc gcattgcttt ttaccgtatt gtctaacttt cttgagcaat
     7081 cgcggtttat tgccctactg atgtatatct tacgcagcag cgtccgcgac agcgtctgcc
     7141 gcattattca aagcgatatt cagcattact ggaatctgcg aattgtcgcc agttcgctat
     7201 gtttaagccc cagcctgctc aaaaagaaat taaaaaacga aaataccagc tatagccaga
     7261 ttgtcacaga gtgtcgtatg cgttacgccg tacagatgtt attgatggat aacaaaaata
     7321 tcactcaggt agcgcaatta tgtggctata gcagcacgtc gtactttatc tctgttttta
     7381 aggcgtttta cggcctgaca ccgttgaatt atctcgccaa acagcgacaa aagtgatgtg
     7441 gtgaagggca aagcggaaac ggataagacg gggataaatg aagaagaaat ggcgcgccct
     7501 gcaggattcg aacctgcggc ccacgactta gaaggtcgtt gctctatcca actgagctaa
     7561 gggcgcgttg ataccgcaat gcggtgtaat cgcgtgaatt atacggtcaa cccttgctga
     7621 gtcaatggct tttgatctgg ttgctgaaca agtgaacgac cgcgtctgat tttctgattt
     7681 attcgctata gcggcaaaca aacgcacatc gctgcgcgtc tgaatcaaga aaacccgtat
     7741 tttcatgtat caaagtacaa tttcccgccc taacggaaaa ttgtccgatc caatgagact
     7801 ggtaactatg aaaccaacgt cggtgatcat tatggatact catcctatca tcagaatgtc
     7861 tattgaagtt ctgttgcaaa aaaacaatga attgcaaatt gtcctgaaaa cggatgatta
     7921 tcgcataacc atcgattatc tccgaacccg tcctgttgat ttaattatta tggatataga
     7981 cttgcccgga acagacggtt ttaccttcct gaaaaggatc aaacaaatcc agagcacagt
     8041 gaaagtatta tttttatcat cgaaatcaga atgcttttat gctggcagag cgatacaagc
     8101 tggtgctaac ggttttgtca gtaaatgcaa tgatcagaat gatatttttc acgccgttca
     8161 gatgatcctc tccggataca ctttttttcc cagcgaaacg ctcaactata taaaaagcaa
     8221 taaatgcagt acgaatagtt caacggtcac ggtgctatct aatcgtgaag tgaccatatt
     8281 acgttatctg gttagcggat tatccaataa agaaattgcc gataagttat tacttagcaa
     8341 taaaacagtt agtgcgcata aatcta
//
LOCUS       DACBVI010000176         8355 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.177, whole genome shotgun sequence.
ACCESSION   DACBVI010000176 DACBVI010000000
VERSION     DACBVI010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.177"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(119..325)
                     /locus_tag="IU325_19900"
     CDS             complement(119..325)
                     /locus_tag="IU325_19900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2517 family protein"
                     /protein_id="HAP5811267.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSEKPVWMDQAEKATGDFY"
     gene            638..727
                     /gene="kdpF"
                     /locus_tag="IU325_19905"
     CDS             638..727
                     /gene="kdpF"
                     /locus_tag="IU325_19905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HAP5811268.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            727..2400
                     /gene="kdpA"
                     /locus_tag="IU325_19910"
     CDS             727..2400
                     /gene="kdpA"
                     /locus_tag="IU325_19910"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HAP5811269.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFSALGVSDREMNWKQYLSAILGLNILGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVAGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTAPGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAILNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2423..4471
                     /gene="kdpB"
                     /locus_tag="IU325_19915"
     CDS             2423..4471
                     /gene="kdpB"
                     /locus_tag="IU325_19915"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HAP5811270.1"
                     /translation="MSRKQLALFEPTLAVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCICIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVKAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKMLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSHVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4480..5052
                     /gene="kdpC"
                     /locus_tag="IU325_19920"
     CDS             4480..5052
                     /gene="kdpC"
                     /locus_tag="IU325_19920"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HAP5811271.1"
                     /translation="MRGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFSGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASANVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5045..7729
                     /gene="kdbD"
                     /locus_tag="IU325_19925"
     CDS             5045..7729
                     /gene="kdbD"
                     /locus_tag="IU325_19925"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdbD"
                     /protein_id="HAP5811272.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGEKLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7726..>8355
                     /gene="kdpE"
                     /locus_tag="IU325_19930"
     CDS             7726..>8355
                     /gene="kdpE"
                     /locus_tag="IU325_19930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HAP5811273.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDITVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARP"
BASE COUNT         1736 a         2176 c         2449 g         1994 t
ORIGIN      
        1 atatatattt cggttgggta atgcgatgta taaatactta cctgaacgtc ttcccgacaa
       61 aaaaaccgcc agcgtttccg ctggcggttt tcttttcttt gaacgtaata tttagcaatc
      121 agtaaaaatc accagttgcc ttttcggcct gatccatcca caccggtttc tcgctggttt
      181 tcgaccagac gcggtgcagg tagctgtaaa agcgggcgcg gtctttccag aaaagcatga
      241 aaggcagcgc cagaacgccc gctgcaaccg cataagtacg gcgtaaaaag ataagccatg
      301 caggatattc tctgtagagt tccatacttt tctccccctt ttgtatctac ccggtgaatg
      361 gcaccggaaa aatgaatttg tttatctgat gaaaatagta ccgccttttg tgtaatttta
      421 ctactcatcc gaccacttat ttttgcttat tgatggttta ttgacattca cactgtaatt
      481 aagttacaca aaagttaaat taatactaac catcagttaa atcatggctt ttgccatttt
      541 tatacttttt ttacaccccg cccgcagatt tttgcgaaat ctttgcagcc agaattctac
      601 ccttccggta tcacttttag gccactggag gtgcactgtg agtgcaggcg tgataaccgg
      661 cgtgttgctg gtgtttttat tactgggtta tctggtttat gccctgatca atgcggaggc
      721 attctgatgg ctgcgcaagg gttcttacta attgccacgt ttttactggt attaatggtg
      781 ctggcgcgtc ccttaggtag cgggctggcg cggctgatta atgatattcc tcttcctggt
      841 acgacgggcg ttgaacgcgt actctttagc gcgcttggcg tctctgaccg cgagatgaac
      901 tggaagcagt atctctcagc cattcttggc ctgaatatac tggggctggc ggtgctgttt
      961 tttatgttgc tcggtcagca ctatctgccg cttaatccac agcagttgcc ggggctgtcg
     1021 tgggatctgg cgctgaatac cgccgtcagc tttgtcacga ataccaactg gcaatcttac
     1081 agcggtgaaa ccacgttgag ctatttcagc cagatggcgg gcttaacggt gcaaaacttt
     1141 ctttctgccg ccagcgggat tgcggtgatt tttgccctca tccgtgcgtt tacccgccag
     1201 agcatgagca cactcgggaa tgcctgggtc gatctgttac gcatcacgtt atgggtgcta
     1261 gtccctgtgg cgttgttgat tgcattgttt tttattcaac aaggtgcgtt gcaaaacttt
     1321 ctgccctatc aggcagtgaa taccgttgaa ggagcgcaac agctgttacc aatgggtccg
     1381 gtggcttctc aggaagcgat caagatgctc ggcactaacg gtggtggctt ctttaatgcc
     1441 aactcgtcgc atccgtttga aaacccaacc gcactgacca acttcgtgca gatgctggcg
     1501 atcttcttga tcccaacagc gctgtgcttt gcctttggtg aagtggcggg cgatcgccgc
     1561 caggggcgca tgttactgtg ggcgatgtca gtgatttttg tcatctgcgt aggcgtggtg
     1621 atgtgggcag aagttcaggg taatcctcat ctgctggcac tgggcgcgga cagcagcatc
     1681 aatatggaag gtaaagagag ccgtttcggc gtgctggtca gtagcctgtt tgcggtcgtg
     1741 actacggcgg cttcctgcgg cgcggtgatt gcgatgcatg attcgtttac cgcccccggt
     1801 ggcatggtgc cgatgtggct gatgcaaatt ggtgaagtgg tattcggcgg tgtcggttct
     1861 ggtctttacg gcatgatgct gtttgtcctg ctggcggtgt ttattgccgg gctgatgatt
     1921 ggtcgtacac cggaatatct gggtaaaaaa atcgacgtac gcgagatgaa actgaccgca
     1981 ctggcgattc tggtcacccc gacgctggtg ctgatgggcg cggcgctggc aatgatgacc
     2041 gacgccggac gtagcgccat cctcaacccg ggcccgcatg gttttagcga agtgctgtac
     2101 gccgtgtcgt ccgccgctaa caacaacggc agcgcctttg ccggattaag cgccaactcc
     2161 ccattctgga actgtttgct ggcgttctgc atgtttgttg gtcgcttcgg ggtgattatt
     2221 ccggtgatgg caattgctgg ttcgctggtg agtaaaaaga gccaacccgc cagctccggc
     2281 acactgccta cgcacggccc gctgtttgtt ggcctgttaa tcggcaccgt gttgctggtt
     2341 ggcgcactga cctttatccc tgccctggcg cttggcccgg tggcggaata tctctcctga
     2401 tgatattgag tgagcactga atatgagtcg taaacaactg gcgctattcg aaccaacact
     2461 tgccgttcag gcgctgaaag aagcggtgaa aaaattaaac ccgcaggcac aatggcgcaa
     2521 tccggtgatg tttatcgtct ggatcggcag tttgctgacc acctgtattt gcatcgcaat
     2581 ggcaagcggt gcgatgcctg gcaatgcgct gtttagcgcg gcaattagcg gctggctgtg
     2641 ggtcaccgta ctgttcgcta atttcgccga ggcgctggca gaaggccgca gtaaagcgca
     2701 ggccaacagt ctgaaagggg tgaaaaaaac tgcctttgcc cgcaagctgc gtgagccgaa
     2761 atatggcgct gcggcggaca aagttcccgc cgatcaactg cgtaaaggcg atatcgtact
     2821 ggtgaaagct ggcgatatta tcccctgcga tggtgaagtc attgaagggg gcgcatcggt
     2881 cgatgaaagc gccatcaccg gggaatcggc accggtgatc cgtgaatccg gcggcgattt
     2941 tgcctccgtc actggcggca cgcgtattct ttctgactgg ctggtgattg agtgcagcgt
     3001 taaccccggc gagacatttc tggatcggat gatcgcgatg gtggaaggcg cgcagcgacg
     3061 taaaacgcca aacgagattg ccctgaccat cctgctgatt gccctgacta tcgtcttttt
     3121 actggcaacc gccacgctgt ggccgttttc cgcgtggggc ggtaatgcag tcagtgtaac
     3181 ggtactggtg gcgctgctgg tctgtctgat cccaacgacc attggcggtc tgttgtcagc
     3241 gatcggcgtc gccgggatga gtcggatgct gggcgcgaat gtgattgcca ccagcggacg
     3301 tgcagttgaa gcggcgggtg acgttgacgt cctgctgctg gataaaaccg gcaccatcac
     3361 gctaggtaac cgtcaggcat cggagtttat ccctgcgcag ggcgtggatg aaaaaatgct
     3421 ggctgacgcc gcacaactgg cttcactggc tgatgaaacg ccggaaggtc gcagtattgt
     3481 gatcctcgcc aagcagcgtt ttaacctgcg cgagcgcgat gtgcagtcgc tccatgccac
     3541 ctttgtaccg tttactgcgc aaagccggat gagcgggatc aacatcgaca accgcatgat
     3601 ccgtaaaggt tctgtcgatg ccattcgccg ccatgttgag gctaatggtg gtcacttccc
     3661 tgccgatgtt gatcaaaaag tcgatcaggt tgcgcgtcag ggagccacgc cgctggtagt
     3721 agtagaaggc tcccatgtgc ttggcgttat tgcgctgaaa gatatcgtca aaggcggtat
     3781 taaagagcgc ttcgcgcagt tgcgtaaaat gggcattaaa acggtgatga ttaccggcga
     3841 taaccgtctg actgccgccg cgattgctgc ggaagcgggc gtcgatgatt tcctcgccga
     3901 agcgacacca gaagccaagc tggcattgat tcgtcagtat caggcagaag gccgtttagt
     3961 ggcgatgacc ggcgacggca ccaacgatgc tccggcgctg gcgcaggcag atgtcgcggt
     4021 ggcgatgaac tccggtactc aggcggcgaa agaagcgggc aatatggtgg atctcgactc
     4081 taacccgacc aagttgatcg aggtggtaca tattggcaaa cagatgctga tgacccgtgg
     4141 ctcgctgacc accttcagca ttgccaacga tgtggcgaaa tacttcgcca ttattccggc
     4201 ggcattcgcg gcaacgtatc cgcagttaaa tgcgctgaac atcatgcgcc tgcattcgcc
     4261 cgactccgca atcctcagtg cggtgatttt caacgccttg attatcgtct ttttgattcc
     4321 cctggcgtta aaaggcgtga gttataaacc gcttaccgct tctgccatgt tgcgccgtaa
     4381 cttatggatt tacggtctgg gtgggctgct ggtgccgttt atcggtatca aagtcattga
     4441 tttactgctg accgtttgcg gtctggtgtg aggtttacca tgcgtggatt acgtccggca
     4501 ttatcgacat ttctcttttt gttactgatt actggcggcg tttacccgct gctgaccacc
     4561 gcgctggggc aatggtggtt tccctggcag gccaacggtt cgttgattcg tgaaggtgat
     4621 acggtgcgcg gttcggcatt aatcgggcag aatttttccg gcaacggcta ttttcatggt
     4681 cgcccgtcgg caacggcaga aatgccctat aatccacagg cttctggcgg gagcaatctg
     4741 gcggtcagta accctgagct ggataaacta atagccgcac gcgttgctgc attacgggcc
     4801 gctaacccgg atgccagcgc gaacgttccg gttgaactgg tgacggcatc ggcaagcggg
     4861 ctggacaata acatcacccc gcaagcggcg gcctggcaga tcccacgcgt ggcgaaagcg
     4921 cgtaatctca gcgttgaaca gctcacgcaa ctgatcgcaa aatacagcca acaaccgttg
     4981 gtgaaatata tcggccagcc ggtcgtcaac attgtcgaac tcaatctggc gctggataag
     5041 cttgatgaat aacgaaccct tacgtcccga ccccgatcgt ctgctggaac aaactgccgc
     5101 gccgcatcgg gggaagctga aagttttctt cggtgcctgt gcaggcgtcg ggaagacctg
     5161 ggcgatgctg gcagaagccc agcgactgcg ggcgcaaggg ctggatattg tggttggcgt
     5221 ggtagaaacc cacgggcgaa aagataccgc cgccatgctg gaagggctgg ctgttctgcc
     5281 gttaaaacgc caggcgtacc gtgggcggca tatcagcgag tttgatctcg atgccgccct
     5341 cgcccgccgt ccggcgctga tcttaatgga cgaactggcg cacagtaatg cgccaggttc
     5401 tcgtcatcct aaacgctggc aggatatcga agaactgctg gaagctggca ttgatgtttt
     5461 cacaaccgtc aacgttcagc atctggaaag tctgaatgat gtggtcagcg gcgtcaccgg
     5521 aattcaggtt cgggaaaccg tgcccgatcc ttttttcgat gccgccgacg acgtggtgct
     5581 ggtggacttg cccccagacg atctgcgcca gcggctgaaa gaaggcaaag tctatattgc
     5641 cgggcaggcg gagcgcgcca ttgaacattt tttccgcaaa ggcaatctga tcgccctgcg
     5701 cgaactggca ctgcgccgta ctgccgatcg cgttgatgag caaatgcgcg cctggcgggg
     5761 gcatcctggc gaagagaaag tctggcacac gcgcgacgcg atccttttat gcatcgggca
     5821 taacaccggc agcgaaaaac tggtccgcgc ggcggcgcgg ctggcgtcac ggctgggaag
     5881 cgtctggcac gcggtgtatg ttgaaacccc tgccctgcac cgcttaccgg agaaaaaacg
     5941 tcgggcaatt ctcagcgcct tacgtctggc gcaggagctg ggcgcggaga cggcaacact
     6001 ttctgatcca gcggaagaga aagcggtagt gcgttatgcc cgtgaacata atctcggcaa
     6061 gattattctc ggtcgcccgg cctcgcgccg ctggtggcgt cgggaaacgt ttgctgaccg
     6121 actggcgcga atcgcccccg atctcgatca ggtgctggtc gcgcttgatg aaccacccgc
     6181 ccgcacgata aacaacgcgc cggatagccg ctcttttaaa gacaagtggc gggtacagat
     6241 tcagggatgc gtggttgccg ccgcgttatg cgccgttatc accttaattg ccatgcagtg
     6301 gctgatggcg tttgatgccg ccaacctggt gatgctgtat ctgcttggcg tggtggtggt
     6361 ggcgctgttt tatggacgct ggccttcggt ggttgccacc gttattaatg tggtgagttt
     6421 cgatctcttt tttatcgccc cacgcggcac gctcgccgtc tctgatgtgc aatatctgct
     6481 gaccttcgcg gtgatgttaa ccgtcgggct ggtgatcggg aaccttactg cgggtgtgcg
     6541 ttatcaggcg cgggtagccc gttaccgcga gcaacgcaca cggcacttat atgaaatgtc
     6601 gaaagctctg gctgtgggcc gcagtccgca ggacatcgcc gccaccagcg aacaatttat
     6661 tgcctccacg tttcatgccc gcagtcaggt gttgttgccc gatgacaacg gtaaattgca
     6721 gccgttaaca catccgcaag gaatgacgcc gtgggacgat gccatcgcgc agtggagttt
     6781 tgataaaggc ctgcctgcgg gcgcgggcac cgacacgtta cccggtgtac cgtaccagat
     6841 tctgccgtta aaaagcggcg agaaaaccta cggactggtg gtggtagaac cggggaatct
     6901 gcgtcagttg atgatcccgg aacagcagcg cctgctggag acatttacgc tgttagtcgc
     6961 caatgcgctt gagcggctga cgctgaccgc cagcgaagaa caggcgcgga tggcaagcga
     7021 acgagaacag atccgcaacg ccctgctggc ggcgctttcg catgatttac gcacgccgct
     7081 tacggtgctg ttcggtcagg cagaaatctt aacgctcgat ctggcaagcg aaggatcgcc
     7141 tcacgcccgc caggccagcg agattcgcca gcatgtgctg aacacgaccc gactggtgaa
     7201 taatctactg gatatggcgc gaattcagtc cggcggcttt aatttgaaga aagagtggtt
     7261 aacgctggaa gaagtggtcg gcagcgcgct gcaaatgctg gaaccgggtt tatcgtcgcc
     7321 catcaatctt tctctgccag aaccgctgac cttaatccac gttgacgggc cgctctttga
     7381 acgggtgctg attaatctgc tggagaacgc ggtgaaatac gcgggggcgc aggccgaaat
     7441 tggtatcgat gcccacgttg agggcgagaa gctacaactg gatgtctggg ataacggccc
     7501 cggtcttccg ccaggccagg agcagacgat atttgataag tttgctcgcg ggaataaaga
     7561 gtcggcggta ccgggtgtcg ggcttgggct ggcaatttgt cgggcgattg tggatgtaca
     7621 cggcggcacc attaccgcgt tcaaccgacc ggaaggtggt gcctgttttc gtgttacact
     7681 tccccagcaa actgcccctg aacttgaaga atttcatgag gatatgtgac aaacgttctg
     7741 attgttgaag atgaacaggc tattcgtcgc tttctgcgca cggcgctgga gggcgacggt
     7801 atgcgcgtct ttgaggccga aacgctgcaa cgcggcttgc tggaagcggc aacccgtaag
     7861 ccagatttga ttattctcga tctcggcctg cccgatggtg atgggattga gtttatccgc
     7921 gacctgcgcc agtggagcgc ggtgccggtg attgtgcttt ccgcacgtag cgaagagagc
     7981 gataaaatcg ccgcgctgga tgccggagcg gatgattacc tgagtaagcc atttggtatt
     8041 ggcgaattgc aggcccgtct gcgcgtcgct ttacgtcgcc actccgccac cgctgcgccc
     8101 gatccgctgg tgaaattttc cgatattacc gtcgatttag ccgcccgcgt gatccatcgc
     8161 ggtgaggaag aggtgcatct caccccaatt gagtttcgcc tgctggcagt gttgcttaat
     8221 aatgcgggaa aagtgctcac ccagcgccag ctccttaacc aggtgtgggg gccaaacgcg
     8281 gtcgaacaca gtcattattt gcgtatttat atggggcatc tgcgacaaaa actggagcaa
     8341 gatcccgccc gcccc
//
LOCUS       DACBVI010000177         8195 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.178, whole genome shotgun sequence.
ACCESSION   DACBVI010000177 DACBVI010000000
VERSION     DACBVI010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.178"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            335..853
                     /gene="hcp"
                     /locus_tag="IU325_19935"
     CDS             335..853
                     /gene="hcp"
                     /locus_tag="IU325_19935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HAP5811274.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            1063..3204
                     /gene="tssI"
                     /locus_tag="IU325_19940"
     CDS             1063..3204
                     /gene="tssI"
                     /locus_tag="IU325_19940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HAP5811275.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFLYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFACWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIALTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSNQIVKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            3280..7780
                     /locus_tag="IU325_19945"
                     /pseudo
     CDS             3280..7780
                     /locus_tag="IU325_19945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521871.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
     gene            7780..8157
                     /locus_tag="IU325_19950"
     CDS             7780..8157
                     /locus_tag="IU325_19950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811276.1"
                     /translation="MNQYIANYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQI
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSIDDELATVVATGLIEAIVTATDSNSLLWK
                     DVSKFLGDKSKSHALVWKDFGQE"
BASE COUNT         2010 a         2016 c         2584 g         1585 t
ORIGIN      
        1 tttaaggaat atctcaaaaa ttatcaaaat gataattcag aaaggataaa tacaatattt
       61 attcatgatt cagctttggc cattttatgg aggcaatacg gactttgaac agttgggtat
      121 ggttatccag atatatatta ttaacttatt gctgaataag agatttaatg attttaaaaa
      181 tagtttttac gacaaccgta aattctagct cggtttgtat gtaaaaaata ctgaaaaatg
      241 atgcggttcc gtgaagtgag ttgcatctgc ctgtctggga ttaacggcag gtcattgtaa
      301 gtagtaatta accacgttaa caaggagttt acaaatgcca acaccgtgtt atatctccat
      361 cactggtcag acccagggaa atattactgc aggtgcattt accgctgatt ctgtcggcaa
      421 catctatgtg caggggcacg aagacgagat gctggtgcag gaattcctgc ataacgttac
      481 tgtcccgacc gatcctcagt ccggccagcc tgcgggtcag cgtgctcaca agccgttcat
      541 ttttaccgtg gcgctgaaca aagctgttcc gctgatgtac aacgcactgg cctccggcga
      601 aatgctgcca accactgaac ttcactggtg gcgtacctct gtggaaggca aacaggagca
      661 ttatttcacc acccgcctga ccgattcgac gattgtcgat atgaaactgc atatgccgca
      721 ttgccaggat ccggcgaaac gcgagttcac ccagttgctc gaagtttcac tggcctaccg
      781 caaaatagag tgggaacacg tcaagtctgg cacctccggt gccgacgact ggcgcgcacc
      841 gctggaagca taagttaagc caacagcatc cggctattag gccggatgct gtttttgtat
      901 tacgccgtaa aaagaaaggt tgtcagtaag gggttacgtt cacgggagat ggcggatgcc
      961 ggaaacttcg gcggtagcgt gcctgaacgc aagcgtaaca ctgtactgtt acagccgctt
     1021 taacagcgaa ctgtctgttt ttccttaaac ccggaggttg ctatgtcaac cggattacgt
     1081 ttcacgctgg aagtggacgg cctgccaccg gacgcgtttg cggtggtctc ctttcatctg
     1141 aaccagtcac tctcttcgct cttttccctc gatctctctc tggtcagcca gcagtttctc
     1201 tcccttgaat ttgcgcaggt gctggacaaa atggcctacc tgacggtatg gcagggcgat
     1261 gacgtacagc gccgggtgaa aggtgtggtg acctggtttg aactggggga gaacgacaaa
     1321 aaccagatgc tgtacagcat gaaggtgtgc ccaccgctgt ggcgcacagg gctgcgccag
     1381 aacttccgta tcttccagaa tgaggacatc gaaagcatac tcgcgacgat cctgaaagaa
     1441 aacggggtga ccgagtggag tccgctgttc agcgagccgc atccttcccg tgagttttgt
     1501 gtccagtacg gcgaaaccga ttacgatttc ctgtgccgga tggcggcgga ggaaggcatc
     1561 ttcttttatg aggagcacgc gcaaaaaagt accgaccaga gcctggtcct gtgcgacacc
     1621 gtgcgttatc tgccggagtc ctttgagatc ccctggaatc cgaacacccg taccgaggtg
     1681 agcaccctat gcatcagcca gttcctctac agcgcacaaa tccgcccatc ttccgtggtg
     1741 accaaagact acacctttaa acgacccggc tgggcagggc gttttgatca ggaaggccag
     1801 caccaggatt accagcgcac acagtatgaa gtgtatgact accccggacg tttcaagggt
     1861 gcccacgggc agaactttgc ctgctggcag atggatggct ggcgaaacaa cgcagaagtg
     1921 gcgcgcggaa caagccgttc gccggagata tggccgggac ggcgaattgc gctgacgggg
     1981 catccgcagg cgaacctgaa ccgggaatgg caggtggtgg caagtgaact gcacggcgag
     2041 cagccgcagg cggtgccggg acgcaggggt tcaggtacca cgctgaataa ccactttgcg
     2101 gtaataccgg cagacagaac atggcggcca cagccgttgc tgaaaccgct ggtggacggc
     2161 ccgcagagcg ccgtcgtgac gggaccggca ggagaagaaa tcttctgtga tgaacatggc
     2221 cgcgtgcggg tgaaatttaa ctgggaccgc tataacccgt caaaccagga cagttcatgc
     2281 tggatccgtg tggcacaggc gtgggcaggc accggctttg gcaacctggc gataccgcgt
     2341 gtgggtcagg aagtcattgt ggacttcctc aacggcgatc cggaccagcc gattattatg
     2401 gggcgcacct accaccagga aaaccgcacc cccggcagcc tgccgggaac aaagacgcag
     2461 atgaccattc gttcaaaaac ctataagggc agcgggttta atgaactgaa gtttgacgat
     2521 gcgacaggga aagaacaggt ctacatccac gcgcagaaga acatgaacac cgaggtgctg
     2581 aataaccgca ccactgatgt gataaacaac catgccgaaa aaataggtaa caaccaggcg
     2641 atcaccgtta ccaataacca gatccagaac attggcgtta atcagataca gacggttggt
     2701 gtcaaccagg tggaaacggt aggcagcaac cagattgtca aagtgggttc aaaccaggtt
     2761 gaaaaggtgg ggatcattcg tgcgctgacg gtgggcgtgg cttaccagac gacggtaggc
     2821 ggcatcatga atacctcggt ggcgttgttg cagtcctcac aggtagggct gcataaatca
     2881 ctgatggtgg gaatgggcta cagcgtcaat gtagggaata acgtcacctt ctcggtgggc
     2941 aagacgatga aggaaaacac cggacaaaca gcagtttatt ctgccggtga gcatctggaa
     3001 ctctgctgcg gtaaggcaag gttggtgctg acgaaggacg gaagcatatt tctcaatggt
     3061 acgcacattc atctggaagg agagtcggat gtgaacggtg atgcgccggt gattaactgg
     3121 aactgtggtg ccacacaacc tgtaccggat gcgcctgtgc cgaaagattt accccccgga
     3181 atgccggata tgcggcaatt ttgagtcagg tgcaaaaaat taaaacggaa ctggtgcagt
     3241 gaccagataa accatattaa aagcagaagg aattgattca tgggtggaaa accggcggcg
     3301 cggcagggtg acatgacccg caagggactg gatattgtgc agggttcagc aggggtgctg
     3361 ataggtgcgc caaccggtgt ggcctgctcg gtgtgtccca caggggaagc cacgccgaaa
     3421 tatggcaatc cggtaaaccc gctgctggga gcgaaggtgc tgcctgctga aaccgacctg
     3481 gcactgtccg gcccgctgcc gtttgttctt ttccgcgcct acagcagcta ccggaccagg
     3541 acgcccgcgc cggtgggggg ggttggtccc ggctggaaag cccccttcga tatccgctta
     3601 cagatacgcg atgaaggcct gatactcaac gacagcggcg gcaggagcat tcactttgaa
     3661 cccctgtttc ccggcgagat aagctacagc cgcagcgagt cgttctggct ggcgcggtgc
     3721 ggggtgctga aacagcacaa aggccacccg cttgcgcggc tctggcgggc gttgccggaa
     3781 gcggtccgtc tgagtccaca catctatatg atggctgcca gcacaaccgg gcagtggctg
     3841 gtgctcggct ggccggagcg ggtgccgggg gcggacgagg tgctgccgcc ggagccgccc
     3901 gcataccggg tgctgacggg cgtggtggac ggcttcgggc ggacgctggc cttccaccgg
     3961 gcggcagagg gtgacgtggc gggtgcggtg acgggggtga cggacggcgc gggccgctgt
     4021 tttcacctgg cgctgagcac gcaggcgcag cgggcggaag ccttccgtaa acagcgcgcc
     4081 tcttctttat cttctccagc cggtccccgt tctgtttcct cttctcaggt tttccccgat
     4141 acgctgcccg ccggtacaga atacggtgcc gataacggta tccggctgga ggcggtgtgg
     4201 ctgacacacg acccggcata cccggatgaa cagccgactg cgccgctggc gcgctatacg
     4261 tacacggcca gcggagaact gcgggcggtg tatgaccgca gcgggacgca ggtgcgcggg
     4321 tttacttatg atgcggagca cgccgggcgg atggtggcgc accattatgc agggaggccg
     4381 gagagccgct accggtatga tgataccggg cgggtgacgg agcaggtcaa cccggagggg
     4441 ctggactacc gctttgagta cgggcaggac cgtgtgacca tcacggacag cctgaaccgg
     4501 cgggaggtgc tgtacacgga aggcgggggt ggcctgaagc gtgtggtgaa gaaggagcat
     4561 gccgacggga gcatcacccg cagcgagtat gatgaggcgg gggaggctga aggcgcagac
     4621 ggatgcggca ggacggcgga cggagtaccg gctgcatatg gcgtctggaa agctgacttc
     4681 agtgatactg ccggacggca ggacggtacg gtatggttac aacagccagt tgcaactgac
     4741 ttcagtgaca tatccggatg gtttacgcag cagccggaaa tatgaccgtc agggccgact
     4801 ggcagaagag atctcgcgca acggtaacat tacgcgctgg ttttatgact ccccccgcag
     4861 tagcctgccc ggcgcggtgg aagacggaat cggggtgacg cggagaataa cgcgaaaccg
     4921 gtacggtcag cttcgggcct tcacggactg ctcggggtac atgacgcggt atgagtatga
     4981 ccggtacggt caacaaatcg ccgttcaccg ggaggaaggc atcagcactt acagcagtta
     5041 taacccgcga ggccaactgg tcagtcagaa ggatgcgcag gggcgtgaaa cccgttatga
     5101 gtacagcgcc gcaggcgacc tgacagcaac gatatcaccg gacggtaaac gcagcaccat
     5161 cgaatatgat aaacgcgggc gtccggtgag tgtgacggaa ggcggtctga cgcgcagcat
     5221 ggggtatgac gctgccgggc gcatcaccgt gctgaccaac gagaacggca gccagtccac
     5281 gttccggtat gacccggtgg acaggctgac ggagcagcgc ggctttgacg gcaggacgca
     5341 gcgttaccgg tacagcgcca cagggcagct tgtccacagt gaagacgagg gacttatcac
     5401 cctctggcat tacgatgcgt cggaccgcat cacgcaccgg acggtgaacg gcgacccggc
     5461 ggagcagtgg cagtatgatg agcacggctg gctgacagaa atcagccata tcagcgaagg
     5521 ccaccgtgtg gcggtccact acggccacga cgataaaggc cgcctgacgg gcgaacggca
     5581 gacggtggag aacccggaga cgggggagat gctgtgggag catgagacga agcacgcgta
     5641 cagcgaacag gggctggcga cccgtcagga gccggacggt ctgccgccgg tggagtggct
     5701 gacgtatggc agcggttatc ttgcgggaat gaagctgggt ggtacgccac tggtcgagta
     5761 cacgcgggac cggctgcacc gtgagacggc ccgcagcttc ggcggggagg catatgagct
     5821 tgccaccgcc tggaatacca gcggccagct ccggagcagg cacctgaacc ttccgcagct
     5881 tgaccgtgac tacgactgga acgacaacgg acagctaatt cgcatcagcg gcccgcagga
     5941 gagccgggag taccgttaca gcgacacggg aaggctgacg ggcgtccaca ccacggcagc
     6001 gaacctggac attgatatcc cgtatgcgac ggacccggca ggaaaccggc tgccggaccc
     6061 ggaactgcac ccggacagca ccctcacggc atggccggat aaccgcatca cggaagatgc
     6121 gcactatgtc taccgctacg atgaatacgg caggctggcg gagaagacgg accgtatccc
     6181 ggaaggggtg atacggatgc acgacgagcg gacgcaccac taccattacg acagccagca
     6241 ccgcctggtg ttctacacgc ggatacagca cggcgagcca caggtggaga gccgctacct
     6301 ctatgacccg ctgggccgcc ggacgggaaa acgggtgtgg cggcgggagc gtgacctgac
     6361 ggggtggatg tcgctgtcgc gtaaaccgga ggtgacctgg tacgggtggg acggcgacag
     6421 gctgacgacg gtacagaccg acaccacgcg tatccagacg gtataccagc cggggagctt
     6481 cacgccgctc ctgagaatcg aaacagagaa tggcgaacag gcgaaggcgc ggcaccgtag
     6541 cctggcggag gtgttgcagg aggacacggg tgtgacgcta ccggcggagc tggcggtgat
     6601 gctggggagg ctggagcggg aactgcggca gggcagcgtg agtgaagaaa gccagcagtg
     6661 gcttgcacag tgtgggttga cggcggaaca gatggcggcg caactggagg cggaatacat
     6721 cccggagagg aaacttcatc tttaccactg tgaccaccgt gggctgccgc aggcgcttat
     6781 cagcccggaa ggggaaacgg cgtggcgcgg ggagtatgac gagtggggaa acctgctggg
     6841 agaggaaagc acgcagcacc ttcaacagtc actccgtctg ccggggcagc agtatgatga
     6901 ggagtcgggg ctgtactaca accgtcaccg gtactatgac ccgctacagg ggaggtatat
     6961 cacccaggat ccgattgggc tgaagggggg atggaacctg tatgcgtatc cactgaaccc
     7021 ggtgcaacgg ctggatccgc tgggattaag tgcggcgttg ggcgcaggag cattaactgc
     7081 catgggaact ggttctgaag taggtacaga gatcggttcg gtaggaggcc ctccggggat
     7141 gttagtcggt gcggcagtcg gtacggtggt tattggtggt ggtataataa tatacaacaa
     7201 tcgcgaggct gttggggacg cgattgagaa aagtatggag atgcagggtg aagccgaatt
     7261 attaaatgca caaagtcaaa tggaattctg gcgagatccc tatggtgctt tttccagcgt
     7321 gtttggaaat gatgattcat caaaagagaa tccaaatgtc gggaaggatt tgacggagga
     7381 agagaagaac tgcctgggtg gtggcagtac gggaagcccg ggaggatggg gaccagaaga
     7441 tgagaatagc gcaagagatc gtatgcagca tgaacggtat aaagatgaat tgcgacagaa
     7501 gatgggaaaa cctgacgtta cagattcaaa tttgaaaaat attatagacg atctttatcg
     7561 gccaaatgca aaagtaggaa gtggaagcac agcagatgct gttcgttatg aattggccac
     7621 aggtgaaaaa gttggtggta gaggacatgt attgaaagca caggattata caagggcttt
     7681 acaggattgg ttggataaga atccaacagc atctccaggg gatcgagcag cagcagaaaa
     7741 cgttcttcag gatttacaga atgctttggg aggcaagtaa tgaaccaata tattgctaat
     7801 tatatagaaa aaatggcatc gttttccact gttttgtctg agacagtctc attagctaat
     7861 gaatactggc atccagaaac tccaccggtg gttatgttgt tctctcagat tggaaaaacg
     7921 attgtaaaaa atttttatga aatgggtgat attgaaagag aaaaaatatt taaacatgtc
     7981 gaagatggta tggtctcgat tgatgatgaa ttggccacag ttgtagctac agggcttata
     8041 gaggcaatag tcactgcgac cgatagcaat tctttacttt ggaaagatgt aagtaaattt
     8101 ttgggtgata aatcaaagtc tcatgcatta gtgtggaaag attttggtca ggagtgatat
     8161 tctttaagaa gtattaagtt cgctattgag gcctt
//
LOCUS       DACBVI010000178         8149 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.179, whole genome shotgun sequence.
ACCESSION   DACBVI010000178 DACBVI010000000
VERSION     DACBVI010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.179"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            88..1584
                     /gene="ravA"
                     /locus_tag="IU325_19955"
     CDS             88..1584
                     /gene="ravA"
                     /locus_tag="IU325_19955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HAP5811277.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            1578..3029
                     /gene="viaA"
                     /locus_tag="IU325_19960"
     CDS             1578..3029
                     /gene="viaA"
                     /locus_tag="IU325_19960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HAP5811278.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTLVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3034..4026)
                     /gene="asnA"
                     /locus_tag="IU325_19965"
     CDS             complement(3034..4026)
                     /gene="asnA"
                     /locus_tag="IU325_19965"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HAP5811279.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQTLLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4178..4636
                     /gene="asnC"
                     /locus_tag="IU325_19970"
     CDS             4178..4636
                     /gene="asnC"
                     /locus_tag="IU325_19970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HAP5811280.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4726..5169
                     /gene="mioC"
                     /locus_tag="IU325_19975"
     CDS             4726..5169
                     /gene="mioC"
                     /locus_tag="IU325_19975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HAP5811281.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5548..7437
                     /gene="mnmG"
                     /locus_tag="IU325_19980"
     CDS             5548..7437
                     /gene="mnmG"
                     /locus_tag="IU325_19980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP5811282.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7501..8124
                     /gene="rsmG"
                     /locus_tag="IU325_19985"
     CDS             7501..8124
                     /gene="rsmG"
                     /locus_tag="IU325_19985"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP5811283.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
BASE COUNT         2038 a         2096 c         2087 g         1928 t
ORIGIN      
        1 cccctttgag actattttag ccaccagcaa gaaacgtcta tactcgcaat ttacgcagaa
       61 cttttgacga aaggacgcca cttcattatg gctcaccctc atttattagc ggaaagaatt
      121 tcccgcctga gcagttcgtt ggaaaagggg ctttatgaac gtagccacgc catccgcttg
      181 tgtttattag cggcattaag tggtgaaagt gtgttcctcc ttggcccgcc aggtattgcc
      241 aaaagtttga tcgcccggcg cttaaaattc gcctttcaga atgcccgcgc gtttgaatat
      301 ctgatgaccc gcttctccac gccggaagaa gtttttggtc ccctttctat tcaggcgcta
      361 aaagatgaag ggcgctatga acgtttaacc agcggttacc tgccggaagc agaaatcgtc
      421 tttctggatg agatctggaa agcgggcccg gcaattctta ataccctgct caccgccatt
      481 aacgagcgcc agttccgtaa cggtgcacac gtagaaaaaa tcccgatgcg cctgctggtg
      541 gcggcctcca acgagctgcc ggaagcagac agcagtctgg aagcgttata tgaccgcatg
      601 ctgattcgtc tgtggttaga taaagtgcag gataaagcga atttccgctc catgctgacc
      661 agtcaacagg atgaaaacga caatccggtt cctgcctcct tgcaggtcac agatgaagaa
      721 tatgaacgct ggcagaaaga gattggtgaa attacgctgc ccgatcatgt atttgagctg
      781 atttttatgc tgcgccagca actggataaa ttaccggatg cgccttatgt ctcggatcgt
      841 cgctggaaaa aagcgattcg attattgcag gccagcgcct tttttagcgg tcgcagtgct
      901 gttgccccgg ttgatctcat tttgctgaaa gattgcctgt ggtatgacgc gcaaagcctg
      961 aatttgatac aacaacaaat tgatgtattg atgaccggtc atgcctggca acagcaaggg
     1021 atgttgaccc gccttggcgc gattgtgcaa cgtcacctgc aactccagca gcaacaaagc
     1081 gataaaacag ccttaacggt aattcgtctg ggcggcattt tcagccgtcg tcagcagtat
     1141 caacttcctg ttaacgttac tgcttccact ctgactctgc tgctgcaaaa accgttaaaa
     1201 ttgcatgata tggaagtggt tcatatctcc tttgagcgta gcgcgctgga acagtggctg
     1261 agtaaaggtg gtgagattcg cggcaaactg aacggtatcg gctttgccca gaaactgaat
     1321 ctggaagtgg atagcgccca acatcttgtt gtacgcgatg taagtttaca aggcagtacg
     1381 ctggcacttc ccggttcatc ggctgaaggt ctgccaggtg aaataaaaca acaactggaa
     1441 gagcttgaaa gcgactggcg caagcaacac gctttattca gcgaacagca aaaatgtctg
     1501 tttatccctg gcgactggtt aggtcgcatt gaagccagcc tacaggatgt cggcgcacag
     1561 attcgccagg cacaacaatg ctaacgctgg atacgcttaa tgtgatgctg gccgtcagcg
     1621 aagagggatt gatcgaagag atgatcatcg cgctgctggc ctcaccgcag ctggcagtct
     1681 tctttgaaaa attcccacga ctgaaggcgg caatcactga tgatgttccc cgctggcgtg
     1741 aggcgctgcg cagtcggctg aaagatgccc gagtcccgcc ggaactcacc gaagaggtga
     1801 tgtgctatca gcaaagccag ctcctctcca cgccgcagtt tattgtgcag ctaccacaga
     1861 tcctggactt actgcatcgt ctgaattccc catgggcaga acaagcccga cagttggttg
     1921 atgctaacag cacgatcact tcagcgttac acacgctttt tctccagcgt tggcgtttaa
     1981 gtctgatcgt gcaagcaacg acgttaaatc aacagctatt agaagaagaa cgcgaacaac
     2041 tgttgagtga agttcaggaa cgcatgacgc tgagcggaca acttgaaccg attctcgcag
     2101 ataacaatac cgcagctggt cgtctgtggg atatgagcgc cggtcagctt aaacgtggcg
     2161 actatcagtt gattgtgaaa tacggtgaat ttcttaacga acagccggaa ctgaaacgcc
     2221 tggcagaaca gctggggcgt tcccgggaag ccaaatcaat accgcgcaac gatgcgcaga
     2281 tggaaacctt ccgcaccctg gtgcgcgaac cggcgacggt tcctgagcag gttgatggtc
     2341 tgcaacaaag cgatgatatt ttacgtctcc tgccgccaga actggcgaca ctagggataa
     2401 cagaactgga gtatgagttt taccgtcggc tggtggaaaa acagttgctc acctatcgcc
     2461 tgcacggtga gtcgtggcga gaaaaagtga tcgaacgtcc ggtggtacat aaagattacg
     2521 acgaacagcc gcgcggaccg tttattgtct gtgtggatac ttccggctca atgggcggct
     2581 ttaatgaaca gtgtgcgaaa gcgttctgcc tggccttgat gcgcattgct ctcgctgaaa
     2641 accggcgctg ctatattatg ttattttcca ccgagatcgt ccgttatgag ctttcaggcc
     2701 cacaaggcat cgaacaagca atccgttttt taagccagca gtttcgtggc ggcaccgatc
     2761 tggccagttg ttttcgcgcc attatggaac gcttgcaaag ccgggaatgg tttgatgccg
     2821 atgcggtggt gatttctgat tttatcgccc agcggttgcc tgacgacgtg acgagtaaag
     2881 tgaaagagct acagcgggta catcagcatc gctttcatgc cgtggcgatg tcggcacatg
     2941 gcaaacccgg catcatgcgc attttcgatc atatctggcg ctttgatacc gggatgcgaa
     3001 gccgcctgct cagacgctgg cggcgataaa ttattacagc agagaaggga cgctctcgcg
     3061 aacagctgcc ggccatacac cacactgaac ctggccgata tgtggcagtt gcagcagcag
     3121 catggtcaaa cgagactgcc cgataccgcc gccgatggtc tgcggcattt caccgcgcag
     3181 cagcgtctga tgccactcca gctccaggcg atcttcgtca ccggtcagcg ccagctgatg
     3241 cttcaacgta tcggcatcca cgcggatccc catggaagaa agctcaaacg catcttccag
     3301 taccgggttc cataccagaa tatcgccgtt cagacccgca tggcccagct ctgacggggt
     3361 gctccagtca tcataatccg gtgcgcgcac gtcgtgacga tgaccatcac tcaacttgcc
     3421 gccaatcccg acgaggaata ccgcgccaag atctttcgca atcgcccgct cacgcccttt
     3481 ggcatcgaga tccggataac gagacagtaa ctcctggctg tgtacgaagt ggatctgatc
     3541 cggcaggaac ggtgccaggc caaactcttc gctaaccgca gcttcggttg ctttaattcc
     3601 cgcccagatc gcctctaccg tgcttttcag agtcgagaat tgacgctcgc cgtcgcccat
     3661 tacgcgttcc cagtcccact ggtctacata gaccgagtgc aacggagaaa gacggtcttc
     3721 atcggggcga agggctttca tgtgcgtgta cagcccttcg cccgcgctga agtcgtgttg
     3781 ccctaaggtc tgacgtttcc acttcgccag tgaatgaacc acttcgaact gggcatcagg
     3841 cagagctttt acttttacct gcaccgcttt ttcacagccc gacaagttat cctgcgtgcc
     3901 atcccccaca cggctaagaa tcggtgcctg gacttcgatc agccccagac gttcttccag
     3961 ttgacgagaa aagtgagatt tcacgaagct aatttgacgt tgtttggcaa tgtaagcggt
     4021 tttcattttt tatactcctg cgtcctgttg cttatgatta agcaacaaaa accgacacac
     4081 atgcaataat cattcaataa aaagcctcat gcacttttga ttcattaaaa aatagggcta
     4141 aaatagaatg aatcatcaat cctcataaga aaatcctatg gaaaattatc tgatcgacaa
     4201 tctggaccgt ggcatcctgg aagcattaat gggcaatgcg cgcaccgctt acgccgaact
     4261 ggcgaaacaa tttggcgtca gtccggggac aattcacgtt cgagtagaga aaatgaagca
     4321 ggcggggatc attaccgggg cgcgtattga tgtcagcccg aagcagctcg gttatgacgt
     4381 aggctgcttt atcggcatta tattaaagag cgccaaagac tatccttccg cgctggcaaa
     4441 gctggaaagc ctcgatgaag tcactgaagc ctattacaca actggccact acagcatctt
     4501 tatcaaagtg atgtgccgtt cgatcgatgc tctccagcat gtacttatca acaagatcca
     4561 aacaattgat gaaattcagt ccaccgagac actgatcgtc ctgcagaacc cgatcatgcg
     4621 taccatcaag ccctgatcgg cttttttaat cccatacttt tccacaggta gatcccaacg
     4681 cgttcacagc gtacaatacg ccactcttaa taaaggtggc ggtttatggc agatatcact
     4741 cttatcagcg gcagcaccct cggcggtgcc gaatatgtag cagaacacct ggctgaaaag
     4801 ctggaagagg cgggttttac caccgaaacg ctgcacggtc cgctgttgga agatttatct
     4861 gcctcgggga tctggctggt tatcagctca acccacggtg ccggagatat tccggacaac
     4921 ctttctcctt tctatgaagc attgcaggaa cagaagcccg atctttctgc ggtccgcttt
     4981 ggcgctatcg gtattggcag tcgtgaatat gacacctttt gtggggctat cgataaactc
     5041 gaggcagaac tcaaaaattc cggtgcaaaa cagacaggcg aaactctgaa gatcaacatc
     5101 cttgatcacg acattcctga agatccggca gaagaatggc tgggatcgtg gattaattta
     5161 ctcaaataag tatacagatc atgcgatcta ctgtggataa ctctgtcaga aagcttggat
     5221 caaccggtag ttatccaaag aacaacggtt gttcagtttt tgagttgtgt ataagccctc
     5281 attctgatcc cagcttatac ggtccaggat caccgatcat tcacagttaa tgatcctttc
     5341 caggttgttg atcttaaaag ccggatcctt gttatccaca aggtagtgcg atcctaataa
     5401 gagatcacaa tagaacagat ctctaaataa atagatcttc tttttaatac ccaggatccc
     5461 aggtctttct caagccgaca aagttgagta gaatccacgg cccgggcttc aatccatttt
     5521 cataccgcgt tatgcgaggc aatcaccatg ttttatccgg atccttttga cgtcatcatc
     5581 attggcgggg gtcatgcagg caccgaggcc gcgatggccg cggcgcgtat gggtcaacag
     5641 actctgcttt tgacacacaa tatcgacact ctggggcaga tgagctgcaa cccggcgatc
     5701 ggcggtattg ggaagggaca tctggtaaaa gaagtggatg cactcggcgg tctgatggcg
     5761 aaagcgatcg atcaggcggg tatccagttt aggatactaa acgcaagcaa gggaccagcg
     5821 gttcgcgcta cccgtgctca ggcggatcgt gtgctctacc ggcaggcggt acgtacggcg
     5881 ctggagaacc aaccgaacct gatgatcttc cagcaggcgg ttgaagatct tattgtcgaa
     5941 aacgatcgcg tggtcggtgc tgttacccaa atgggactga agttccgtgc caaagccgtc
     6001 gtgctcaccg ttgggacgtt cctcgacggt aaaattcata tcggtctgga taactacagc
     6061 ggtggtcgtg ctggtgatcc gccgtccatt ccgctctctc gccgtttacg tgaactgccg
     6121 ctgcgcgttg gtcgtctgaa aaccgggaca ccgccgcgta ttgatgcgcg tactatcgac
     6181 ttcagcgtgc tggcgcaaca gcatggcgat aacccaatgc cggtattctc gtttatgggt
     6241 aatgcgtccc agcatccaca gcaggtgccg tgttatatca cccataccaa cgagaaaacc
     6301 catgatgtga tccgcagtaa cctcgatcgt agcccaatgt atgcaggggt gatcgaaggt
     6361 gtcggcccac gctactgccc gtcgatcgaa gacaaagtca tgcgcttcgc cgacagaaat
     6421 cagcatcaga tcttccttga accggaaggg ctgacctcaa acgaaattta tccgaacggt
     6481 atctccacca gcctgccgtt cgatgtgcag atgcaaatcg tccgctccat gcaggggatg
     6541 gaaaacgcga agatcgtacg tccggggtat gccattgagt atgacttctt cgatccacgc
     6601 gacctgaaac cgacgctgga gagcaagttt atccaggggc tgttctttgc tggtcagatt
     6661 aacggcacta ccggttacga agaagccgct gcgcaaggtt tgctggctgg tcttaacgct
     6721 gcccgtctgt ctgctgacaa agaaggttgg gctccggcgc gttctcaggc gtatctcggc
     6781 gtactggttg atgacctgtg cactttagga accaaagaac cgtatcgtat gtttacttcg
     6841 cgcgcagaat atcgtctgat gctgcgcgaa gataatgcgg atctgcgttt gactgaaatc
     6901 ggtcgtgaac tgggcctggt ggatgacgaa cgttgggcgc gctttaacga gaaacttgag
     6961 aatatcgagc gtgagcgtca gcgtctgaaa tcgacctggg taaccccgtc ggcggaagct
     7021 gcagccgaag tgaatgctca cctgactgcg ccgctttccc gtgaagccag tggtgaagat
     7081 ctgctgcgtc gtccggaaat gacttatgaa aaattaacca cgctgacgcc gtttgcccct
     7141 gcgttgacag acgaacaggc ggcggaacag gttgagattc aggttaaata cgaaggttat
     7201 atcgcgcgcc agcaagatga gatcgaaaag cagctgcgta acgagaacac cctgctacca
     7261 gcgacgctgg attaccgcca ggtatccggt ctttctaacg aagtgatcgc caaacttaac
     7321 gatcacaaac cggcctctat cggccaggct tcgcgtattt ctggcgtcac gcctgcggcc
     7381 atctccattc tgctggtatg gctgaaaaaa cagggtatgc tgcgtcgtag cgcataacgc
     7441 attaaaaatg cctggtaagc acccgcttac cgggcaacgc atcaagaaca ggtaatcacc
     7501 gtgctcaaca aactctcctt actgctgaaa gacgcaggta tttcgcttac cgatcaccag
     7561 aaaaaccagc ttattgccta tgtgaatatg ctgcataaat ggaataaagc gtacaacctg
     7621 acttcggtcc gcgatcctaa tgagatgctg gtacgccata ttctcgatag cattgtggtg
     7681 gcaccgtatc tgcaaggtga acggtttatc gatgtcggca ccggaccagg actgcctggc
     7741 attccactct ctatcgtgcg tcctgaagcc cacttcacgt tgttggatag ccttggtaaa
     7801 cgcgtgcgtt tccttcgtca ggtgcaacat gagcttaaac tggagaatat tgagccagta
     7861 cagagcaggg tagaagagtt tccttcagag ccgccatttg atggcgtaat tagccgcgct
     7921 tttgcctctc tgaacgatat ggtgagctgg tgccaccatc ttcctggtga gcaaggccgt
     7981 ttctacgcgc tgaaagggca aatgccggaa gatgaaatcg ctttgttgcc cgaagaatat
     8041 caggtcgaat cagtggttaa acttcaggtt ccagccctgg atggcgaacg tcatctggtg
     8101 gtgattaaag caaataaaat ttaattttta tcaaaaaaat cataaaaaa
//
LOCUS       DACBVI010000179         8108 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.180, whole genome shotgun sequence.
ACCESSION   DACBVI010000179 DACBVI010000000
VERSION     DACBVI010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.180"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..426)
                     /locus_tag="IU325_19990"
     CDS             complement(<1..426)
                     /locus_tag="IU325_19990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811284.1"
                     /translation="MPNTSIHLSPCNILQNNKLQPTEIYKESQQTAKLEFFCDEFLKI
                     SQSRYGLSTSADSIANLLAFFTKASDAIDSIKTQKIDVHSYGFVPIRHFVEHVITYKN
                     EFAADYEPYTLTFTRGENNEGILSVESKEGFISQRTINLN"
     gene            complement(892..1770)
                     /locus_tag="IU325_19995"
                     /pseudo
     CDS             complement(892..1770)
                     /locus_tag="IU325_19995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            1922..3112
                     /locus_tag="IU325_20000"
     CDS             1922..3112
                     /locus_tag="IU325_20000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5811285.1"
                     /translation="MKEKLWTKDFWAITIISFIIFFVFYVLLTLLPIYISDRLHASPD
                     KAGLLVTLFLIAAIVIRPFAGQWVGKYSNKTILVLSSLAFLLVTALYPFCHSIESLLF
                     IRVLHGITFGVITTVKGTISARLIPASRRGEGISFFSLAMGLAMVVGPWIGLNMARWE
                     AFNLAFWLCTGVAAVGIILSLIMTVPPVISHADGSTPKMGFAAMFDRAALPFAMVTFF
                     MTFSYAGVSAFLALYARELNLMSAASNFLLCYAIFLMICRTFTGNVCDKKGPKYVVYP
                     CLVFFTVGLVVLGYTQGSIMMVVSGALIGIGYGSVTPVFQTQIISSVEPHKIGVANSL
                     FFNAMDAGLALGACVMGMMVAHTGYRMIYLLGALLVVVAGGVYALQMKGKSGVALVVA
                     KEIH"
     gene            3193..3843
                     /gene="inaA"
                     /locus_tag="IU325_20005"
     CDS             3193..3843
                     /gene="inaA"
                     /locus_tag="IU325_20005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HAP5811286.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(3897..4151)
                     /gene="yfaE"
                     /locus_tag="IU325_20010"
     CDS             complement(3897..4151)
                     /gene="yfaE"
                     /locus_tag="IU325_20010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HAP5811287.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4151..5281)
                     /locus_tag="IU325_20015"
     CDS             complement(4151..5281)
                     /locus_tag="IU325_20015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase 1 subunit
                     beta"
                     /protein_id="HAP5811288.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            complement(5370..7655)
                     /gene="nrdA"
                     /locus_tag="IU325_20020"
     CDS             complement(5370..7655)
                     /gene="nrdA"
                     /locus_tag="IU325_20020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HAP5811289.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         1982 a         1843 c         2042 g         2241 t
ORIGIN      
        1 atttaagttt attgttcttt gtgaaataaa cccttctttg ctttcgacac tcaaaatacc
       61 ttcgttgttt tcacctcggg taaaggtaag ggtatagggt tcataatccg ctgcaaattc
      121 atttttatac gttatgacat gttcgacaaa atgtcttatt ggtacgaaac cataactgtg
      181 aacgtcaatt ttttgggtct tgatactatc aattgcatct gaggctttcg tgaaaaaagc
      241 caataaatta gctatgctgt ctgctgaagt tgataacccg tatctacttt gagatatttt
      301 tagaaactca tcgcaaaaaa actcaagttt cgcggtttgt tgtgattctt tgtaaatttc
      361 tgttggttgc aatttattat tttgtaaaat attacaggga gataagtgaa tagatgtatt
      421 tggcataaat gattccttgt attgtgagtt aacaagatac aatctaatct ttgcaaaata
      481 tattcactat caaatttagc tcattttatt acgaagacct ttacgcatgg tagaaagtat
      541 cattattgtt aataaatacg gtaataagta acaaacagat attgataact tccctgaaaa
      601 gttactaggt atcccacttg aagattgaga atggctccag agtaaactgt gtactgttcg
      661 ccggagtgtc attactctca acgatcggtt ttgacagtga gtcgggcgct tcataccgat
      721 ttctacctgg agtcattgtc acttctccca actggataaa tatatatcgc caattcacaa
      781 agtgggccaa aatcaggtga aaacgaatta ttacgcctgg taaaggctat agtacaacta
      841 agtgtggcga atcaggcagt cttgcatttg tcatcaatct catgtcttac atcaaatttt
      901 tccactcacc actaattgtt ctgtgcacat ttgtagtaaa aacagtggac tgccttctac
      961 cggcttttct ttagccagca acgaaatctg tcgatagaag gttggttcga gaggaacgga
     1021 acacaattca tctgcaatgg ttttcagcgt taactctggt aataaggcga tgcctaatcc
     1081 ctgtcgaata aaactgatcg ctgtatccgg gtgattaaat tcatatttta tctgtggggt
     1141 aatattcttt tctttaaata gtgccataat gcttaattca tagcgtcctt tactaataat
     1201 taatggttca cccattaact cttccactgt aatagtgctg ttttttgcca gagcatggtc
     1261 gggcggtaac actacggtga atttatcctg ataaatgggc acggagtaca ttccgctcac
     1321 cggaaaatga acaaaaccgg catcaatgga ttcattctga agcgaatcaa taatcgctgt
     1381 gctattttcc tcaaaaggaa taatttttat attcggatga tgaatttcaa agtagcgaat
     1441 cacaaccggt aagatacagg cgcagacgct ggggaaacaa ccaagtctca gcgttctttg
     1501 agggttctgc ttttcctgct cggctatctc ttttactgcg ttaacttccc gattaatggc
     1561 gcgcagatgt tgcacaatgc gatgacctgc tgtggtcagt tgtatagctt tacggcgatc
     1621 gcgtaacagt atattcacac caagttcttg ttcgaggcta gcaattgcct gacttaccgc
     1681 cgactgcgtc atgaactatc gcttgctggc ttcagtaaag ccaccgtaat ccagcacggc
     1741 gagtaaagag tttatttggg taagagtcat tagagtttgc taatcaataa tattaaaacg
     1801 attaatttct ctaatagcat gcagccattt atacttctgt tctgcaaagt acatcacaat
     1861 ttgacgcaac cgcagtaacg ggagtgttgt ctgcgtgcgt catcctgaca ggcggtaggg
     1921 tatgaaagag aagttatgga cgaaggattt ttgggcaata accatcatca gctttattat
     1981 tttcttcgtc ttttatgttt tactaacatt gttgccaatt tatatctctg accgcttgca
     2041 tgcctctcct gataaagccg gtttattggt gactttattt ttaattgccg ccattgttat
     2101 tcgcccattt gccgggcaat gggtgggtaa atactcgaat aaaactattc tggtgctctc
     2161 ttctctggcc tttttattgg tcacagcgtt gtatcctttt tgccactcaa ttgaatcact
     2221 gctttttatt cgggtgcttc atggtattac cttcggggtt atcactacgg taaaaggaac
     2281 gatttccgcg cggctgatcc cggcctcccg acgtggggag ggcatcagtt ttttctctct
     2341 ggcaatgggg ctggcaatgg tggtcgggcc gtggattggt ctgaatatgg cgcgctggga
     2401 agcctttaat ctggctttct ggctatgcac cggcgtggca gcggtgggga ttattctgtc
     2461 gctgattatg acagtgccgc cggttatcag ccatgctgat ggttcaacgc cgaaaatggg
     2521 cttcgccgcc atgttcgatc gcgcggcgtt gccatttgcg atggtcacct tttttatgac
     2581 cttttcgtat gccggggttt ctgcttttct ggcgctttat gcacgcgaac ttaacctgat
     2641 gtcggcagcc agtaatttcc tgctttgcta cgctatcttc ctgatgatct gccgtacctt
     2701 taccggcaat gtctgcgaca aaaaaggacc gaaatatgtg gtttaccctt gccttgtgtt
     2761 ctttacggta ggactggtgg ttctcggcta cacccaggga agcataatga tggttgtttc
     2821 tggcgcattg attggtatcg ggtatggttc cgtgacgcca gtttttcaga cgcagattat
     2881 cagttcagtg gaaccgcata aaatcggtgt cgcaaactcc ctcttcttca atgcgatgga
     2941 tgcaggcctg gcgctgggag cctgtgtgat ggggatgatg gttgcacata ctggctaccg
     3001 aatgatttat ctgctgggtg cactattagt ggtagtggct ggtggagtct atgcgctgca
     3061 aatgaagggt aaaagcggtg ttgcgctagt agtggcaaaa gaaattcatt aatacgacac
     3121 gtttcattaa gatttttcgc aggtaaccac ctatagtcat tgtcgttaat aaaaaatgac
     3181 aggcgatagg gtatggcagt ttcagcaaag cacgacgaat ttaatcactg gtgggcaaca
     3241 gagggcgact gggttgaaga gcctaactat cgccgcaatg gtatgagcgg ggtgcaatgc
     3301 gtcgagcgca acggcaaaaa gctatatgta aagcgcatga cgcatcatct gtttcattcc
     3361 gtacgttatc cgttcggccg accaacgatt gtccgtgagg ttgcggtaat taaagaactg
     3421 gaacgggccg gtgtcatcgt gccgaagatc gtttttggtg aagcggtgaa aattgagggg
     3481 gaatggcgcg cgttgctggt gactgaagat atggcggggt tcatcagcat tgctgactgg
     3541 tatgcccagc atgcagtaac gccctattct gacgaagtac ggcaagccat gttgaaagca
     3601 gtggcgctgg cgtttaagaa aatgcatagc gttaatcgtc agcatggctg ttgctatgtt
     3661 cgccatatct atgtgaaaac agaaggtaag gcagaagctg gatttcttga tctggagaaa
     3721 agtcgtcgcc gcttgcgtcg cgataaagcg attaatcatg acttccgcca gctggaaaaa
     3781 tatctggagc cgatccccaa agcggactgg gagcaggtga aagcgtatta ctacgccatg
     3841 taatcgttgt aggccagata atacgcgtaa gcgtcgtatc tggcgctagt acccgatcac
     3901 aactcgattt caatatcgcc ttttgcccgg caacaacagg gcaaaatttc ccccggctga
     3961 ataaaggcta acggttcggc aatccagtcc acctggcctg caacaaggcg cgtgcgacag
     4021 gaaccgcagt aaccttcgcg gcactggtac tcaaccgcca cattgtggga ttccagcgcc
     4081 gccagaaggg aagggtgttc atcctggcac agcagttgtg tgccagtgat gcgcagggta
     4141 acgcgagcca tcagagctgg aagttactca aatcgtcggt gtccacttcc gagtcaatct
     4201 gcccgaccag ataagaactg acttccactt cctgcggagc aacctgcacg ttatcagaca
     4261 ccagccaggt attgatccac gggatcgggt tggagcgcgt ctggaacggc agatccaaac
     4321 cgactgcctg catgcggata ttggtgatgt attcaacgta ctggcagaga atgtctttat
     4381 tcagaccaat catcgaaccg tcgcggaaca gataatccgc ccagtctttc tcttgctgtg
     4441 ccgcctggac aaacaggtca tagcactcct gcttacattc ttcggcaatt tccgccatct
     4501 ccggatcatc cgcaccgctg cgcagcagat tcagcatatg ctgagtaccg gtcaggtgca
     4561 gggcttcgtc gcgggcaatc aggcgaataa ttttggcgtt accttccatc agttcgcgtt
     4621 ctgcaaaggc aaaggaacaa gcaaagctga cgtagaagcg gatcgcttcc agcgcgttaa
     4681 cgctcatcag gcagagatac agttttttct tcagctcgcg caggctaacg gttacggttt
     4741 taccgttaac ggtgtgggta ccttcgccca gcagatgcca gtagctggtc atttcgatca
     4801 gctcatcgta ataactggag atcccttccg cacgtttctg gatctgctcg ttggtgacga
     4861 tatcatcaaa cacaacggac ggatcgttaa cgatattacg aatgatatgg gtataagaac
     4921 gagagtgaat ggtttctgag aacgcccagg tttcgaccca ggtttccagt tcagggatcg
     4981 agatcagcgg cagcagtgcc acgttcgggc tgcgaccctg aatggagtcc agcagcgtct
     5041 gatatttcag gttgctgata aagatgtgtt tttcgtgttc aggcagtgcc tggtaatcga
     5101 tacggtcacg agaaacgtca acttcttccg gacgccagaa gaaagagagc tgcttttcga
     5161 tcagcttttc gaagatgtca tatttttgct gatcgtagcg agccacgttg accggctgac
     5221 caaagaacat cggttctttg agctgatcat ttttcgtctg tgaaaaggtg gtatatgcca
     5281 tgaatgtgtc ctgtggtttt attacccctc accctaaccc tctcccgcaa ggggagaggg
     5341 gacggtttgg tgcaaaggct gggggattat cagatcttgc atgcgccgct ttcgcagcca
     5401 tcgtcctgga ttgacggcac cagatcgtct tgtgcgtctt cagcaccgtc acgggtgttc
     5461 tgataataca gcgttttgac cccgaatttg taggcggtga gcaggtcttt cagcagttgc
     5521 tgcatcggca cttttcctga cgggaagcgt gacggatcgt agttggtgtt agcagagatc
     5581 gactgatcga taaatttctg catgataccc accagttgca ggtagccatc gttacccggc
     5641 atttcccaca gcagctcata ggcgtcgtgc agatgctcgt agtccggcac cacctggcgg
     5701 agaataccgt ctttcgacgc tttgatgctg acgtaaccgc gcggcggttc aataccgtta
     5761 gtggcgttag agatctgcga agaagtctct gacggcatca gagcagaaag cgtggagtta
     5821 cgcaggccgt gcgttttgat tgactcacgc agtgcttccc agtcgtaatg cagcggctca
     5881 ttagcgatgg catccagatc tttcttatag gtatcgatcg gcaggatccc tttcgcgtaa
     5941 gtggtttcgt taaaccacgg gcacgcgcct tgctctttcg ccagctcatt agaggctttc
     6001 agcaggtaat actgaatagc ttcgaaggtt ttatgtgtca ggttgttggc gctgccgtcg
     6061 gagtagcgtt taccgtgctt cgccaggtag taagcgaagt tgatcacacc aatacccagc
     6121 gtacgacgac ccatcgctcc acgtttggcg gccgggatcg ggtaatcctg ataatccagc
     6181 agcgcgtcga gtgcgcgtac tgccagaatc gccagctctt ccagttcatc caggctatta
     6241 attgcgccca ggttgaaagc agacaacgta cacagcgcga tttcaccgtt ctcgtcgttg
     6301 acatcattca gcggtttggt cggcagggcg atttccaggc acaggttaga ctggcgcact
     6361 ggcgcgatgg ccggatcaaa cgggctatgg gtattgcagt ggtcaacgtt ctgaatatag
     6421 attcgaccgg tagacgcacg ttcctgcatc atcagcgaga acagctcaac ggctttcaca
     6481 cgctgcttgc ggatgctgtc gtctttctca tatttggtgt acaggcgttc aaactcttcc
     6541 tgatcggcga agaacgcgtc gtacagcccc ggtacgtcgg acgggctgaa cagggtgata
     6601 tcttcacctt tcagcagacg ggtatacatc agtttgttga tttgtacccc gtagtccata
     6661 tgacgcacgc ggttgccttc caccccacgg ttgtttttca acaccaacag gctttccact
     6721 tccagatgcc acatcgggta gaacagcgta gccgcaccgc cacgcacacc gccctgagag
     6781 caggatttca ccgctgtctg gaaatgctta tagaacggaa tacaaccggt atggaacgct
     6841 tcgccaccgc gaatcgggct acccagcgca cgaatacgcc cggcattgat gccgatcccg
     6901 gcacgctggg aaacatattt cacaatcgcg ctggaggtgg cgttgatgga atccaggctg
     6961 tcgccgcact cgatcagtac gcaggagctg aactgacgag tcggggtacg cacgccggac
     7021 atgattggcg tcggcagcga aattttaaat gtggaaaccg cgtcgtaaaa acgcttcacg
     7081 tattgcaggc gcgtttcacg cgggtagttc gagaacaggc acgcggcaac cagaatataa
     7141 aggaactggg cgctttcgta gatttcgccg gtcacgcggt tctgtaccag atatttgcct
     7201 tccagctgct taacggcagc ataagagaag gtcatatcac ggtcgtgatc gataaacgtg
     7261 tccatctgct tgaactcttc ttccgtgtag tcttccagca gatgattatc gtatttgccc
     7321 atctcgacca ttttcaccac gtggtcgtac agcgcaggcg gctcaaactg gccgtaggct
     7381 tttttacgca ggtggaagat cgccaggcgc gcggcgagat actgataatc cggcgcatca
     7441 cgggagatca ggtctgcggc agccttgata atggtttcgt ggatgtcaga ggtcttgata
     7501 ccgtcataaa actgaatgtg ggagcgcagt tcgacctggg aaatcgaaac gttatgcagt
     7561 ccttctgccg cccaatccag aacgcgatgg attttgtcga gattgatgcg ctctgtgcta
     7621 ccgtcgcgct ttgtcaccag cagattctga ttcatgtatg tcgtacctgt ttttggaatt
     7681 ctttccgcaa tagtttcatg cagactacct gtagtaaggg aagctgctta gaacactata
     7741 tatagggggt atgtttggga ttcactgcaa gatagtgtga aaatggccct cttgcaagtg
     7801 cataactttg tggataactc aggaaggaaa aagtggcttt cgcgcacctt aggtcagaca
     7861 aggtgtccgg gaaagtcaat gggaagagaa aaatttgtta aaaataactg ttcggcgatt
     7921 tcttgaacgg aagaataggt tagataaatt gatatagatg gtttattaat cttttggcta
     7981 atgattgcaa aaattgatct aagcacgagt catcacataa tctgatgtat cagttatggt
     8041 caatatataa gcgaatgtaa gcgattgttg atgtcgaaat ggttgttttc tcttcggttg
     8101 ttgttaaa
//
LOCUS       DACBVI010000180         8036 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.181, whole genome shotgun sequence.
ACCESSION   DACBVI010000180 DACBVI010000000
VERSION     DACBVI010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.181"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(9..2957)
                     /gene="ehaB"
                     /locus_tag="IU325_20025"
     CDS             complement(9..2957)
                     /gene="ehaB"
                     /locus_tag="IU325_20025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HAP5811290.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTDCADRADDYYHDRLALTVDNSTIDDNYEHYTYNGTYNN
                     AADTHVVNVFNIGTAITLDQEVDLSISNNSHVAGITLTQGYEWEDIDDNTVSTGVNSS
                     EVFNNTITVKDSTVTSGSWTDEGTTGWFGNTGNASDYNGKGWNADDIALAVIAHPAAD
                     NAMQTTATFDNSTLMGDVFFSSNFDENFFPHGRDSYRDADGDVDTNGWDGTDRLDLTL
                     NNGSKWVGAAMSAHQIGVDTDDDGVNDSYTYGINTEATATLIDIAANSLWPSSTVGVE
                     NSDSEYSEFDHIIGNEVYQSGLFNVTLNTGSQWDTTKTSLIDTLSINSGSTVNVADST
                     LISDSISLTGLSALNINEDGHVATDSLTVDNSTVTISDEVSAGWAVGDAALYANNIKV
                     TNDGILNVGNTAANALQVDTLNLTSTTDTSGNIHAGVFNIESNRFVLDADLTNDRTND
                     TTKSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVAAATGNYKVRI
                     DNATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRGNTVVLQQMEL
                     TDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTI
                     NYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRTGQVDQDSQTAYIYSSAH
                     FANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVT
                     PYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLA
                     YVYDDSNNDNDVNGDSINNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDW
                     SANVGVKYTW"
     gene            3483..4457
                     /gene="hemB"
                     /locus_tag="IU325_20030"
     CDS             3483..4457
                     /gene="hemB"
                     /locus_tag="IU325_20030"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HAP5811291.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(4497..5348)
                     /gene="tauD"
                     /locus_tag="IU325_20035"
     CDS             complement(4497..5348)
                     /gene="tauD"
                     /locus_tag="IU325_20035"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HAP5811292.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRAQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWSSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLSFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(5345..6172)
                     /gene="tauC"
                     /locus_tag="IU325_20040"
     CDS             complement(5345..6172)
                     /gene="tauC"
                     /locus_tag="IU325_20040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HAP5811293.1"
                     /translation="MSVLINEKLHSHRLKWRWPLSRQVTLSIGTLAILLTVWWAVAAL
                     HLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(6169..6936)
                     /gene="tauB"
                     /locus_tag="IU325_20045"
     CDS             complement(6169..6936)
                     /gene="tauB"
                     /locus_tag="IU325_20045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HAP5811294.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESCELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKCIEGPGAERGVVFQNEGLLPWRNVVDNVAFGLQLAGVEK
                     VQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFITMREYVLSRVFEQREAFS"
     gene            complement(6949..7911)
                     /gene="tauA"
                     /locus_tag="IU325_20050"
     CDS             complement(6949..7911)
                     /gene="tauA"
                     /locus_tag="IU325_20050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP5811295.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1989 a         2116 c         1975 g         1956 t
ORIGIN      
        1 gaagaatatt accaggtata tttaacaccc acgttcgcgg accagtcttg atctacgtca
       61 ccaccaccga ggtagttagc atcggtatag gcgctgaagt tcttggtgaa gctgaactga
      121 gtacccagac caacacgtac cgcagaccct tcagtaccgt tattgatgga atcaccgttc
      181 acatcgttat cgttgttaga gtcgtcgtag acgtaagcca gtttgaagta cggagtcaga
      241 gcctggtctt cgctgtaggt gaaggtataa cctgcatcta cacccagttc ataacgcatg
      301 ctgtcgtaag actgaccgtc aactttcatg tcgttgctca gctggtagtc atcaccagac
      361 tggaacagac cagaaacgct gccgtaagga gtcacgtaac cagcatcacc cagtttgaag
      421 tcgtaaccgg ctttcaaacc gaagccccaa gcgtcggagt tggtgctacc gtcaacgtaa
      481 gtaccgttgc tcatggttgc agacaggtcg ttgttgaagt gagagtagct taagctacca
      541 tcaacaaaga cgttgttcgc gaagtgagca gaagagtaga tgtaggcagt ctggctgtct
      601 tgatccacct gaccagtacg gtcattcatg tcacctttcg cgaagccagc agccgcaccg
      661 acgatccact tagcgttgtt accgtcaatt ttggtatcaa caccgaccat gatgccgtta
      721 acatcctgat catagttgat ggtgccgttg tcgccgttga agttaccacc gaagtagctt
      781 acccatgcgc cgccgttatc agccaggcca tgacgagagt tggtcagacg agtaccaacg
      841 gtgtcttgtt ccaggttcca gatattggtg ttcgcagacg ggatgctcag cgccatgtta
      901 gcgtagtcgg tcagctccat ctgttgcaga acaacggtgt taccacgctg ttcagcctga
      961 taggtgtatg cacccaggtc agctttgtta gcagcagaga aggtcgcgtt gctgtttttg
     1021 tcgttaacgt agatcagctc gttgccgttg tagtcagcga tagaaccagc acctgtagcg
     1081 ttgtcgatac gaactttgta gttaccggtt gcagctgcaa catggtcacc gttgttatca
     1141 acttcgttct gaccagcttc gatagaagca gtgttgtcgt tatcgccgtt accgttaatg
     1201 gtcaggtgac catcagagtt cattgcgatt aagccataac cgtagtttga cttggtagta
     1261 tcgttggtac ggtcgttggt caggtcggca tcaagtacga agcggttgct ttcgatgttg
     1321 aacacaccag cgtgaatgtt accactagta tcagtagtgc tggtcaggtt cagagtatca
     1381 acctgcagag cattcgccgc agtgttaccg acattcagaa taccgtcgtt agtcactttg
     1441 atgttgttgg catacagagc agcatcacct acagcccaac cagcagaaac ttcatcagaa
     1501 atggttacgg tactgttgtc gacagtcagt gaatcagttg caacatgacc atcttcgttg
     1561 atgttcagcg cagaaagacc tgtcagagag atagagtcag aaatcagcgt tgaatcagca
     1621 acattaaccg ttgaaccact gttgatgctc agagtatcaa tcagagaagt tttggtggta
     1681 tcccactgtg aaccggtgtt cagggttaca ttgaacagac cgctctggta aacttcgtta
     1741 ccgatgatgt gatcaaattc gctatattca gagtcgctgt tttctacacc aacagttgat
     1801 gaaggccaca ggctgttagc tgcgatgtcg atcagagttg cagtggcttc agtattgatt
     1861 ccatatgtat aggagtcatt tacaccatcg tcatcagtat caacaccaat ctgatgcgca
     1921 gacattgccg caccgaccca cttgctgcca ttgttcaggg tcagatccag gcgatcagta
     1981 ccatcccaac cgttagtatc tacatcaccg tcagcatcac gatagctatc acgaccatgc
     2041 gggaagaagt tctcatcaaa gttacttgag aagaatacat cgcccatcag tgtggaatta
     2101 tcgaatgttg cagtggtttg catggcgtta tcagcagcag gatgtgcaat aacagctaat
     2161 gcgatatcgt cagcattcca acctttacca ttgtaatcgc tggcattacc tgtattaccg
     2221 aaccaaccag ttgtaccttc atcagtccat gaaccagagg tcacggttga atctttaaca
     2281 gtgatggtgt tattaaacac ttcgctgctg tttacaccag tgctgacagt gttgtcgtcg
     2341 atatcttccc actcataacc ctgagtcagc gtaatacctg ctacatgaga gttattagag
     2401 atggacagat caacttcctg atccagagtg atggcagtac caatgttaaa tacatttaca
     2461 acatgagtgt cagcggcatt attgtaggtg ccgttatatg tgtagtgttc gtagttgtca
     2521 tcgatcgttg aattatcaac ggtcagcgcc agacgatcat ggtagtagtc atccgcacga
     2581 tcagcacaat cagttgtcat gcactcggaa taaatcatgc cgtgaatagt gctgtttttg
     2641 attgtcaggc tattggcgtt ggtattagta gaaataccgt catccagata gtaggtagaa
     2701 atcacgccgt taacggtagc gttattaatg accggataga tatcgccgtt gtaataacta
     2761 tcagcggcat agttattcca gcctacataa ccatcatagt aaacgtcatc agcataggtg
     2821 gcgtcgttgt agtgatggaa agtattgtaa gtttgaccag aaatatcgtt ggtcgctgca
     2881 ttagcctgag aggtgatagc cagagtgcaa gccaatgcta attgtgatac tacaagtttc
     2941 tttttccagg agtgcatttg tcatccctcc tcagggacgt aataagttga tccatcaatt
     3001 atcaatgcat agaaaacata ttatgtttcg cgttgacgat tatgcggcgt tatagaggaa
     3061 tggttcaatt attctttccc ctaagtccga ttgcggacat atataaagca cgtgaacaca
     3121 cacacagaaa gaaataacaa aaacacaata tttcaatacg ttaatgaaag cgttaatttt
     3181 aaaacataga ccatgaaaat ataaaacaac caaaacagac aaataatgta tcaataagca
     3241 atgaacttta agttattcac tttttgaaaa tgtcaccaaa tccccaaaaa caataatcta
     3301 tggatataaa taaaaattaa aaatattttc agtgaatata aaattattgc tatttctatt
     3361 acaaagatgt cctggcctct cttccattct cttcttgtca acatcgcgac aactttcgta
     3421 aaacatcccc accctgcttc aggtatacta tgcccctcga ttccacaaac atcaggcaga
     3481 ccatgacaga cttaatccaa cgccctcgtc gcctgcgcaa atctcctgcg ctgcgcgcta
     3541 tgtttgaaga gacaacactt agccttaacg acctggtgtt gccgatcttt gttgaagaag
     3601 aaattgacga ctacaaagcc gttgaagcca tgccaggcgt gatgcgcatt ccagagaaac
     3661 atctggcacg cgaaattgaa cgcatcgcca acgccggtat tcgttccgtg atgactttcg
     3721 gcatctctca ccataccgat gaaaccggca gcgacgcctg gcgggaagat ggactggtgg
     3781 cgcgtatgtc gcgcatctgc aagcagactg tgccagaaat gatcgtcatg tcagacactt
     3841 gtttctgtga atacacttct cacggtcact gcggtgtgct gtgcgagcat ggcgtcgaca
     3901 acgacgcgac tctggaaaat ttaggcaagc aagccgtggt tgcagctgct gcaggtgcag
     3961 acttcatcgc cccttccgcc gcgatggacg gccaggtaca ggcaattcgc caggcgctgg
     4021 acgctgcggg ctttaaagac acggcgatta tgtcgtattc gaccaagttc gcctcctcct
     4081 tttatggccc gttccgtgaa gctgccggca gcgcattaaa aggcgaccgc aaaagctatc
     4141 agatgaaccc aatgaaccgt cgtgaggcga ttcgtgagtc actgctggat gaagcccagg
     4201 gcgcagactg cctgatggtt aaacctgctg gagcgtacct cgacatcgtg cgtgagctgc
     4261 gtgaacgtac tgaattgccg attggcgcgt atcaggtgag cggtgagtac gcgatgatta
     4321 agttcgccgc gctggcgggt gctatagatg aagagaaagt cgtgctcgaa agcttaggtt
     4381 cgattaagcg tgcgggtgcg gatctgattt tcagctactt tgcactggat ttggctgaga
     4441 agaagattct gcgttaatgt tcattatgcc gggttatcca acccggcatt tcccccttac
     4501 cccgcccgat aaaacggttt atccccaagg atcgtcgccc gatgcataat ccgccgctgt
     4561 ggcaggtaat ccgcattggc atagtgctga gtcacgcgat tatcccaaat cgcaatatca
     4621 ttcggttgcc agcgccagcg cacctgaaac tccggtttgg tgatatgggc aaacaaaaaa
     4681 cttaacaagg cttcgctctc tttctcgctg acatcaacaa ttcgcgtagt aaagccttca
     4741 ttcacaaaca gcgcctgttt accgctcacc ggatgcgttc gcaccaccgg atgtagcaac
     4801 ggcgggtttt tcgcgaccgc ctcgcgccag cgttgatgct cctcctcggt tttgcggtat
     4861 ttgtattccg ggaacgattt acggaaatca tgctccgcac gcagcccgct cagcagttgg
     4921 cggaagggaa cagagagcgc ctcataggcc gcaataccgc tgctccagag cgtatcgccg
     4981 ccggtcgagg gtaactcttt tgccgccaga atcgcccctg cgggtggcgt ttcaataaat
     5041 gtcacatcgg tatgccagtt gtcgttatcc ggcggattat cgttatgagt atccagcacg
     5101 atgatctcgt caaccccttc ggcatgaggg taaacagggt gaatatgcag ttcgccaaaa
     5161 cgctgggcca gcgcgcgttg ctgctgcggc gtaatagctt gagcgcgcag aaacaccacc
     5221 tgatggcgca gcaccgcatg gtaaagctgt tcaaactgat tatcgcttaa cgggcgcgtc
     5281 aggtcggcac ccgaaatttg tgcgccaata tacggcccca gcggggtaat gctcagacgt
     5341 tcactcattg tacttctcca tgccagggcg tcaggcggcg ctgtaacgcg cgcaaaccca
     5401 gttctaaaag aaaagcgata atcgcaatca ccgcgatccc cgccagcacc acgtcagtcg
     5461 cgagaaattc acccgctgac tgaaccataa atcctaaacc gcgcgtcgcg gcaatcagct
     5521 ccgccgctac cagcgtagac cagcccacac ccagaccaat gcgtaatccg gtgaggattt
     5581 ccggcagcgc gccgggcaaa atgacaaacc acagcacctg cgcacgactg gcacccagcg
     5641 actgggcggc acgaatgcga acctgctgcg cgcttttcac ccccgccagc gccgacatcg
     5701 ccaccggtgc aaaaatcgct aaatagatca gtaagatctt cgaggtttca ccaataccaa
     5761 accagatcac catcagcggc aaatacgcca gcggcggcac cggacgataa agctcgatta
     5821 tcggatccag aatgccgcgc accgtcgggc ttagtcccat ggcgatcccg accggaatgc
     5881 cgatcaacac cgccgcaagt agcgccagca caatgcgcgt caggctggct gcaagatgct
     5941 gccacagggt ggcgtccata aatccctgcg gcccggcaat ggtgagcagt tttgccagta
     6001 cctgttgcgg tggcggcaaa aacagcgggc taatcaggtg cagcgccgcc actgcccacc
     6061 atacagtgag taaaatcgct aacgtgccaa tgcttaaggt cacctgacgc gagagcggcc
     6121 agcgccattt cagccgatgc gaatgcagtt tttcattaat gagcacactc atgagaacgc
     6181 ctcccgttgc tcaaatacgc ggcttaaaac atattcgcgc atggtgatga attgtggatc
     6241 ggacttgatg ctgcggctcg actctcccgc aacaaagcgg cgagcaaagt tgagcggcag
     6301 ccgctccagc acgcggccag ggccgggtga aagcagaacc agttcagtcg ccataaacac
     6361 cgcttcttct atatcgtggg taatcaacag cacctgcttg cccgtctcct gccagagttt
     6421 cagcagcaga gtttgcatct gatcgcgggt gaaggcgtcc agcgcgccaa acggttcgtc
     6481 gagtaataac agctggggat tcgccgccag cgcgcgggca atccctaccc gctgacgttg
     6541 accgccggaa agctgccaga tatagcgttt ttctgcgcct tccagcccca cttttttcag
     6601 catctggtgc gcgatttcca ggcgctgtac tttctctaca cctgccagtt gcaggccgaa
     6661 cgccacgttg tctaccacat tacgccacgg caacagccct tcattctgaa aaaccacgcc
     6721 ccgctctgcg cccggtccct caatgcactt acccgccagt tgaatgctgc catgctgata
     6781 aggcacaaaa ccggcaatca gattcagcaa ggtggttttg ccgcagccgg acggccccag
     6841 caccaccagt agctcacagc tttccagcgt caggttgata tcttccagtg ccggtttgcc
     6901 gccataatcg gcgtaaagat gagagatttg cagcatctgc gcctcctttt attgcacgaa
     6961 gcgcgaggtc acgtactggc tgtaatcatt cgctacagcc gggactttgc cctgctcttt
     7021 caaaaactgc gcggtgtcga tgatcgcttt gttcaccggt ccggtcagtt ctgccgtttg
     7081 ctgctgcggc gtcagatagg tattcccctt caccagcccc ggaacgtcac cttcaggtac
     7141 gccgcttaaa cgtgccagtt tgctgatgtt ttccggctgt ttcagccacg cgtctggatt
     7201 agcaatgtac ggttgttgag catcgatggc gcttttagcg aacgctttca cgacctcagg
     7261 atgtttctcg gcaaaatctt tgcgcactac ccagacatcc agcgtcggcg cgccccactg
     7321 cccgacctgt tcagaatcgg tcagcacctt gccgtctttt tccagggcgt taaccgccgg
     7381 tgcccagaca taagcaccat caatgtctcc ccgttgccat gcggcgataa tcgcgggtgg
     7441 ctgcaggttc actatctcca cttgtccggg ttttataccc cagtgtttca gcgccgccag
     7501 caggctgtag tgggtggtgg agataaacgg tacggcgatg cgtttgccaa tgagatcttc
     7561 cggtttgctg atagttttct ttaccaccag cgcttcggag ttacccagtt ttgacgccag
     7621 taagaagact tcaatcggca cctgttggct ggctgccacc gctaatgggc tggaaccgag
     7681 gttaccgatt tggacatcgc ctgaagccag cgcccgcacg atgctggctc cgctgtcaaa
     7741 cttacgccag tccaccgtcg ccccgctctc tttagcaaag gtgttatcag cctgagccac
     7801 tttcgccggt tcggcggagg tttgatacgc cacggtgacg ttcaccgcct gtgcctgaaa
     7861 agcgatgaat gccagtgcgg caagaagtgt gttacgcgat gaaattgcca tgattgtctg
     7921 ctcccctgtc ttgttatagg agcagtattc aggaataaaa acattcatta aaagaattag
     7981 tcgttatcgc acagatgatt ttattcttag caaaaaagcg gcaaaggaaa gattaa
//
LOCUS       DACBVI010000181         8030 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.182, whole genome shotgun sequence.
ACCESSION   DACBVI010000181 DACBVI010000000
VERSION     DACBVI010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.182"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            28..741
                     /locus_tag="IU325_20055"
     CDS             28..741
                     /locus_tag="IU325_20055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HAP5811296.1"
                     /translation="MCVNEYIGDCTDEELNDREFIASVVDRAIFHFAINSICNPEDNK
                     DATPIERCTFDVETKNGLPSTVQLFYEESKDNEPLANIHLQAIGSGFLTFVNACQEYD
                     DNSLKLFASLLISLSYSSAYVDLAGAERVKINEYNENYLTAQFEELSQRDMKKYLGEM
                     KRLADGGEMNFDDYLDKMSLLVNEGKLEPDILSKMRDAAPKLIDFAKSFDPNSKEKIK
                     ILTDTSKLIYDLFGVKSEK"
     gene            1104..2141
                     /gene="dusA"
                     /locus_tag="IU325_20060"
     CDS             1104..2141
                     /gene="dusA"
                     /locus_tag="IU325_20060"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HAP5811297.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            2275..2517
                     /gene="pspG"
                     /locus_tag="IU325_20065"
     CDS             2275..2517
                     /gene="pspG"
                     /locus_tag="IU325_20065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HAP5811298.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(2683..3666)
                     /locus_tag="IU325_20070"
     CDS             complement(2683..3666)
                     /locus_tag="IU325_20070"
                     /EC_number="1.6.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HAP5811299.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3749..5164
                     /locus_tag="IU325_20075"
     CDS             3749..5164
                     /locus_tag="IU325_20075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase DnaB"
                     /protein_id="HAP5811300.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            5217..6296
                     /gene="alr"
                     /locus_tag="IU325_20080"
     CDS             5217..6296
                     /gene="alr"
                     /locus_tag="IU325_20080"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP5811301.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGLRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6549..7742
                     /locus_tag="IU325_20085"
     CDS             6549..7742
                     /locus_tag="IU325_20085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HAP5811302.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEI
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGIYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1963 a         1899 c         2083 g         2085 t
ORIGIN      
        1 ttaattaagc aaataaatga tctcaaaatg tgtgtcaatg agtatattgg agattgtact
       61 gatgaggaat taaacgatcg tgaatttatt gcatcagttg ttgaccgggc tatttttcat
      121 ttcgcgatta atagtatatg taatcccgag gataataaag atgcgacgcc catagaacga
      181 tgtacttttg acgtagaaac taagaatggc cttccctcca cggttcagct tttttatgag
      241 gaatctaaag ataatgaacc tttagcgaat atacatttgc aagcaatagg ttctggtttt
      301 ttaacgtttg ttaatgcatg tcaggaatat gatgacaaca gcttaaaatt attcgcttcg
      361 ctgttaattt cactttcata ttctagtgcc tacgtagatt tagcaggagc agaaagagtg
      421 aagattaatg aatataatga gaactatctg acagctcagt ttgaagaatt atctcaacgt
      481 gatatgaaga agtacctggg agagatgaag cgtctggcag atgggggaga aatgaatttt
      541 gatgactatc tggataaaat gtcacttctg gtgaatgaag gaaagctcga acctgatatt
      601 ttaagcaaaa tgcgagatgc tgcaccgaaa ttaattgact ttgctaagtc gtttgaccca
      661 aactcaaagg aaaagattaa aatacttaca gacacttcta aattaattta tgatttattc
      721 ggggttaaat cggagaaata atatgtgaag ttcttcgata gtatggaagg cattatataa
      781 aaggacccaa tgcttattgg gttctttttt tctctatcag tgctattagc agggagagat
      841 atcaccgtag tttaatgtgt gatttttatt tatcgtcgaa cctggattgt ttatcattgg
      901 ccttaacaaa gctaacggct aataagatta tttccatcac ttcgtgagag cttcatgcct
      961 tgagaggatc tcaattttct tttgcaatga gacaggcgct tcctgttgtt atggtatagt
     1021 accccgctat tgagcctcct gaacagtgat gctgaataac ataaccccat gatatatcga
     1081 taaaataatc tctacattta aaaatgcacg gtaattctga aatgcaaaaa atcaaccaaa
     1141 ccagcgcaat gcctgaaaaa actgacgttc actggagtgg tcggtttagc gttgcaccaa
     1201 tgctcgactg gacggacaga cattgccgct atttcttgcg tctgctttcc cgcaatacgt
     1261 tgctgtatac cgaaatggtg actacagggg cgattattca cggtaaaggt gattacctgg
     1321 cgtacagtga agaagaacat ccggtagcat tgcaactggg cggtagcgat ccggcggcgc
     1381 tggcgcagtg tgcaaagctg gcagaagcgc gcggatatga tgagatcaac ctgaatgtcg
     1441 gctgcccgtc tgaccgggtg cagaacggca tgtttggggc gtgtctgatg ggtaatgcgc
     1501 agctggttgc cgactgcgtg aaagcgatgc gcgatgtggt gtcgattccg gtgacggtga
     1561 aaacgcgtat tggcatcgac gaccaggaca gctatgaatt tctctgcgat ttcatcaaca
     1621 ccgtttccgg caaaggcgag tgtgagatgt tcatcatcca cgcacgtaaa gcctggcttt
     1681 cggggttaag cccgaaagaa aaccgtgaaa tcccgccgct cgattatccg cgagtgtatc
     1741 aattgaagcg tgactttccg catctgacaa tgtcgattaa cggtggtatc aagtcgctgg
     1801 aagaggctaa agcgcatttg caacatatgg atggcgtgat ggtcgggcgc gaagcgtatc
     1861 agaatccggg tattctggcg gcggtagacc gagagatctt tggttcctcg gataccgatg
     1921 ccgatccggt ggcggtagtg cgcgccatgt atccgtacat tgagcgtgaa ctcagccagg
     1981 ggacgtatct tggccatatt acccggcata tgctgggctt gttccagggt attcctggcg
     2041 cgcggcagtg gcggcgttat ttaagtgaaa atgcccataa agcgggtgca gacattaatg
     2101 tgctggaaca cgcgctcaaa ctggtggcgg ataagcgtta acttttcacc aaaaaatagt
     2161 caaattcacc acgccctgcg caccgtcgcg gggcgttttg ctgttaaatc aatagattat
     2221 ttttggcatg attcttgtaa tgccagcaag agatttcata tttgggagag catcatgctg
     2281 gaactacttt ttgtgattgg cttttttgtc atgttgatgg tcaccggcgt ttcgttgctg
     2341 ggcattatcg ccgcgctggt tgtggcgacg gccattatgt tcctcggcgg tatgctggca
     2401 ttgatgatta agttgctgcc gtggttactc ctggcgattg cggtggtgtg ggttattaag
     2461 gcgattaaag caccaaaagt gccgaaatat cagcgttatg accgctggcg ttactaaggg
     2521 attgtgcgga tgatcacaac ctaaggtttt atccttagaa caaaatagga attgataatc
     2581 aaatctgtca ctattgcgcc tctaacagat tcatcgtgct gtaccctaca tacagccgaa
     2641 ctataaaaag aaagggcttc ccaggtggaa gccctatttc ttttatggaa tcaacaggct
     2701 ggaaccttgc gtcgcccggc tttccagaat ctcatgcgca cgctgcgcat ccttcagcgg
     2761 atatttctgc tgctcggcga catcgacctt aatcacaccg ctggcaatca aagagaacag
     2821 ttcattactg gcctcggtta attcctcccg cgtggtgata tagccttgca gggaagggcg
     2881 tgtcacatac aacgagcctt tctgattgag aatgcctaag ttcacgccgg taaccgcacc
     2941 tgatgagttg ccaaaactca ccattaagcc gcggcgttgc aggcaatcca gcgaccgttc
     3001 ccaggtgtct ctgcccacgg aatcgtacac cacgcgcact ttcttgccac cggtgatctc
     3061 ttttaaccgc tcgaccagat tctcttcgcg atagttaata acctgccatg cgcccgcttt
     3121 tagcgcactc tgcgcttttt gcgcggttcc cacagtgccg ataagtttcg ccccaagggc
     3181 ttttgcccac tggcaggcaa ttaagccaac gccgccagcc gctgcgtgga acaggaactg
     3241 ctcatcgggt ttaatttcat aggttttgcg cagcagataa taaaccgtta agcctttcag
     3301 gaaggatgcc gcagcttgct caaaagaaat tgccgcaggc agaatcgccg ctttatccgc
     3361 attaatgtta tgcaccgagc tgtaagcgcc taacgccgac tgcgcataga ctacacgatc
     3421 gcctgcctta atatgcttta caccactgcc gactttactc acgatgcctg ccgcttcggt
     3481 acctaatccg ctgggtagtg atggcggcgg gtaaaggccg ctgcggatat acgtgtcgat
     3541 aaaattgatg ccgatggctt tattttcgac ctggatttca ttctccgccg gatcggcagg
     3601 agtgaactct acggcttgaa gtacttccgg gccaccgtgc ttgtgaaatt caattcgtgt
     3661 tgccatgtgt cctccagaac gtatcgtcag ggtctgcttc atatggtaaa gtttcgaccc
     3721 attctttatc tcggtaactc cattcactat ggcaggaaat aaacccttca acaaacagca
     3781 ggctgaaccc cgcgaacgcg atccacaagt tgccgggctg aaagtgcctc cgcactcgat
     3841 cgaagcggag cagtcggtgt tgggcggttt aatgctggat aacgaacgct gggacgatgt
     3901 agccgagcgt gtggtggcag acgattttta cacccgccca caccgtcata tctttactga
     3961 aatggcgcgt ttacaggaaa gcggtagtcc tatcgatctg attacccttg cggaatcgct
     4021 ggaacgccag gggcaactcg atagcgtcgg tggtttcgct tatctggcgg agctgtcaaa
     4081 aaatacgcca agtgcggcga acatcagtgc ttatgctgac atcgtgcgtg aacgtgccgt
     4141 tgttcgcgag atgatttcgg ttgcgaatga gattgccgaa gccggttttg atccgcaggg
     4201 gcgcaccagc gaagatctgc tggacctggc tgaatcccgc gtctttaaaa ttgccgaaag
     4261 tcgtgcaaac aaagacgaag ggccgaagaa catcgctgat gtgctcgacg ccacggtggc
     4321 gcgtattgag cagttgtttc agcagccaca cgatggcgtt accggggtaa acaccggtta
     4381 tgacgatctc aacaaaaaaa ccgctggctt gcagccgtcg gatttgatca tcgtcgccgc
     4441 gcgtccgtcg atgggtaaaa ccacatttgc gatgaacctc gtcgaaaacg cggcgatgtt
     4501 gcaggataaa cctgtactta tcttctcgct ggagatgcca tcagaacaga ttatgatgcg
     4561 ttctctggcg tcgctgtcgc gcgttgacca gactaaaatc cgtaccgggc agctcgatga
     4621 tgaagactgg gcacgtattt ccggcaccat gggtattttg ctcgaaaaac gtaatatcta
     4681 tatcgatgat tcctccggcc tgacgccaac ggaagtgcgt tcccgcgcgc gccgtattgc
     4741 ccgtgaacac ggcggcatcg ggcttatcat gatcgactac ctgcaactga tgcgcgtacc
     4801 ggcgctttcc gataaccgta cgctggaaat tgcagaaatc tcccgctcgc tgaaagcact
     4861 ggcgaaagaa ctgaacgtgc cggtggtggc gctgtcccag ttgaaccgtt ctctggaaca
     4921 acgtgccgac aaacgtccgg tcaactccga cctgcgtgaa tctggctcta tcgagcagga
     4981 tgcggacttg atcatgttta tctatcgtga tgaggtgtat cacgaaaaca gtgatttaaa
     5041 aggcatcgcg gaaattatta tcggtaaaca acgtaacggc ccaatcggga cggtacgcct
     5101 gacctttaac ggtcaatggt cgcgcttcga caactatgcg gggccgcagt acgacgacga
     5161 ataataatta ttttatgaat taggtaatta aagcaaacac ttatcaagga acacaaatgc
     5221 aagcggcaac tgttgtgatt aaccgccgcg ctctgcgaca caacctgcaa cgtcttcgtg
     5281 aactggctcc tgccagtaaa atggttgcgg tggtgaaagc gaacgcttat ggtcacggtc
     5341 ttcttgagac cgcgcgaacg ctccccgatg ctgacgcctt tggcgtagcc cgtctcgaag
     5401 aagctctgcg actgcgtgcg gggggaatca ccaaacctgt actgttactc gaaggctttt
     5461 ttgatgccag agatctaccg acgatttctg cgcaacattt tcataccgcc gtgcataacg
     5521 aagaacagct ggctgcgctg gaagaggcta gcctggacga gccggtaacc gtctggatga
     5581 aactcgatac cggtatgcac cgtctgggcg taaggccgga acaggctgag gcgttttatc
     5641 atcgcctgac ccagtgcaaa aacgttcgtc agccggtgaa tatcgtcagc cattttgcgc
     5701 gcgcggatga accaaaatgc ggcgcaaccg agaaacaact cgctatcttt aatacctttt
     5761 gcgaaggcaa accaggtcta cgttccattg ccgcatcggg tggcattctg ctgtggccac
     5821 agtcgcattt tgactgggtg cgcccgggca tcattcttta tggcgtctct ccgctggaag
     5881 atcgctccac cggtgccgat tttggctgtc agccagtgat gtcactaacc tccagcctga
     5941 ttgccgtgcg tgagcataaa gccggagagc ctgtcggtta tggtggaacc tgggtaagcg
     6001 aacgtgatac ccgccttggc gtagtcgcga tgggctatgg cgatggttat ccgcgcgccg
     6061 cgccgtccgg tacgccagtg ctggtgaacg gtcgcgaagt gccgattgtc gggcgcgtgg
     6121 cgatggatat gatctgcgtc gacttaggtc cacaggcgca ggataaagcc ggggacccgg
     6181 tcattttatg gggcgaaggt ttgcccgtag aacgtatcgc tgaaatgacg aaagtgagcg
     6241 cttacgaact tatcacgcgc ctgacttcaa gggtcgcgat gaaatacgtg gattaatcgt
     6301 tctgtaatat ttgattgttt gtgccggatg cggcgtgaat gccttatccg gccaataaaa
     6361 tcctaccaat tcaataagtt gatattcttt catgctctta tgaaggccgt gcctctggcg
     6421 gatgtacgtt tgtcatgagt ctcactctgt tgctaattgc cgttcgcccc tgaacatcca
     6481 ctcgatcttc gccttcttcc ggtttattgt gttttaacca cctgcccgta aacctggaga
     6541 accatcgcgt gtttcaaaaa gttgacgcct acgctggcga cccgattctt acgcttatgg
     6601 agcgtttcaa agaagaccct cgcagcgaca aagtgaattt aagtatcggt ctgtactaca
     6661 acgaagacgg aattattccc caactgaaag ccgtggcgga tgcggaagcg cgcctgaatg
     6721 cgcagcctca tggcgcttcg ctttatttac cgatggaagg gcttaatagc tatcgccatg
     6781 ccattgcgcc gctgctgttt ggtgcggacc atccggtact gcaacaacag cgcgtagcaa
     6841 ccattcaaac ccttggcggc tcaggggcat tgaaagtggg cgcagatttc ctgaaacgct
     6901 acttcccgga atcaggcgtc tgggtcagcg atcctacctg ggaaaaccac gtagcaatat
     6961 tcgccggggc tggattcgaa gtgagtactt acccctggta tgacgaagcg actaacggcg
     7021 tgcgctttaa tgacctgttg gcgacgctga aaacattacc tgcccgcagt attgtgttgc
     7081 tgcatccatg ttgccacaac ccaacgggtg ccgatctcac taatgatcag tgggatgcgg
     7141 tgattgaaat tctcaaagcc cgcgagctta ttccattcct cgatattgcc tatcaaggat
     7201 ttggtgccgg tatggaagag gatgcctacg ccattcgtgc cattgccagc gctggattac
     7261 ccgctctggt gagcaattcg ttctcgaaaa ttttctccct ttacggcgag cgcgttggcg
     7321 gactttctgt tctgtgtgaa gatgccgaag ccgctggccg cgtactgggg caattgaaag
     7381 caacagttcg ccgcaactac tccagcccgc cgaattttgg tgcgcaggtg gtggctgcgg
     7441 tgctgaatga cgaggcattg aaagccagct ggctggcgga agtagaagag atgcgtactc
     7501 gcattctggc gatgcgtcag gaactggtga aggtattgag cacagagata ccagaacgca
     7561 atttcgatta tctgcttaat cagcgcggca tgttcagtta taccggttta agtgccgctc
     7621 aggttgaccg actacgtgag gagtttggta tttatctcat cgccagcggt cgcatgtgtg
     7681 tcgccgggtt aaatacggca aatgtacaac gtgtagcaaa ggcgtttgct gcggtaatgt
     7741 aatgcaggaa agcaggctgg agttatccag cctgcagtga aattaaactg tcgtcgcttt
     7801 cactcttgct ttatagatga tttttttgat gccatcgttc tacgtgagag gtaataaaac
     7861 gttgttagtt ctaacttgtt aagtttatcc caatgttctg gagtgctttt ccctgtttta
     7921 ccgtgtggag taataatttc tgcacatcta accattatta aatttaataa taagtatcgg
     7981 gtttgttacg atagaatatt tctatatcct caagaatatg ctttgcctta
//
LOCUS       DACBVI010000182         7927 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.183, whole genome shotgun sequence.
ACCESSION   DACBVI010000182 DACBVI010000000
VERSION     DACBVI010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.183"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..914)
                     /locus_tag="IU325_20090"
     CDS             complement(<1..914)
                     /locus_tag="IU325_20090"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP5811303.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTNKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVD"
     gene            complement(1125..2522)
                     /locus_tag="IU325_20095"
     CDS             complement(1125..2522)
                     /locus_tag="IU325_20095"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HAP5811304.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPDHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2927..4576
                     /gene="treF"
                     /locus_tag="IU325_20100"
     CDS             2927..4576
                     /gene="treF"
                     /locus_tag="IU325_20100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HAP5811305.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(4627..5229)
                     /locus_tag="IU325_20105"
     CDS             complement(4627..5229)
                     /locus_tag="IU325_20105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAP5811306.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            5678..6649
                     /locus_tag="IU325_20110"
     CDS             5678..6649
                     /locus_tag="IU325_20110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP5811307.1"
                     /translation="MLEKTINNAICALLFRCEQQSVKEMDKIHAMQLFIKVAELESFS
                     RAADFFALPKGSVSRQIQALEHQLGTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNE
                     LDGLFQQDATSISGKLRIDIPPGIAKSLLLPRLSEFLYLHPGIELELSSHDRPVDILH
                     DGFDCVIRTGASPEDGVIARPLGKLTVVNCASPHYLTRFGYPQSPDDLTSHAIVRYTP
                     HLGVHPLGFEVASVNGVQWFKSGGMLTVNSRENYLAAGLAGLGIIQIPRIAVREALRA
                     GRLIEVLPGYRAEPLSLSLIYPQRRELSRRVNLFMQWLAGVMKEHLD"
     gene            6698..7711
                     /gene="yhjD"
                     /locus_tag="IU325_20115"
     CDS             6698..7711
                     /gene="yhjD"
                     /locus_tag="IU325_20115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HAP5811308.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQR"
BASE COUNT         2005 a         2042 c         1900 g         1980 t
ORIGIN      
        1 tagtcaacgt tgaacaccag ttcctgcggc agcgcttctt catcacgcca gataacccag
       61 ccgcagccca gcggagccag accgaattta tggcctgaag cactgatcga tttcacacgc
      121 ggcaggcgga agtcccagac gatatccggg gcgacgaacg gtgccaggaa gccaccgctg
      181 gcggcgtcga tgtgcatgtc gatgtcgata ccggtgtcgg cctggaattt atccaacgca
      241 tcgtgcagcg gttgcgggaa ctcatagtta ccggtgtagg tcacgccgaa agtcggcacc
      301 acgccgatgg tgttttcgtc gcaggcctca atcatgcgtt tcgggtccat aaacaactga
      361 ccggggcgca tagggatctc acgcagctcc acatcccagt agcgggcaaa tttatgccag
      421 cagatttgta ccggaccgca caccaggttt ggtttgttgg tcggcttacc tgcggcttcc
      481 atacgcttgc gccaacgcca tttcatcgcc atcccgccga gcatacaggc ctcggaagaa
      541 ccaatggtgt tggtgccaac ggcctgacca tttttcggcg caggcgcatg ccacagatcg
      601 gcaaccatgt ttacgcaacg caggtcgatg gctgcagatt gcggatattc ttctttgtcg
      661 atccagtttt tattgatcga caaatccatc aatttatgga cgttttcgtc gtcccaggtc
      721 tggcaaaaag tggccaggtt ctgacgagcg ttgccatcaa gatataattc atcattgata
      781 atctggaatg cgacatcatc gcgcatttcg tgcagcggaa atcgttttga ctccgcgata
      841 gtagaaatgg cctttgcgcc aaaacgtgaa tcgagtagtt ctgagcggaa atccgttaac
      901 agcttctggt ccatttcgaa ctccttaaat ttatttgaag gcaataaaaa agtaggattt
      961 atccgcaatg gaagcaaggc attacaggct taatttaaat aacaaaatcc taagcagaaa
     1021 aacgactttt tataatataa acatttaaca tgataatatt aaaaacagac tttattatcg
     1081 caataaaaat cacccgaaca ttcctgttcg ggcgattaaa ttaattattg tttatcctgc
     1141 atatacggcg tgtaaacccc gttcagactg tgcaggaaag ctacgatatc atccacatcc
     1201 tcctgcggca gctctttgcc tacctgatag cgcaacatca gtttcaccgc cccgtccagc
     1261 gtcggcacat caccgcgatg gaagtacggt gccgttagtg caacgttacg taaaccgggt
     1321 actttctgac gcaatttatc gcgctcttct ttagtaacat tcatacgacc aatatccgcc
     1381 gccgtaattt caccaaagtt aaagtctttt ttcagcccca acggttcgaa ggaacgtccg
     1441 ccgagaataa taccaccatg acaagttgca catttattat ctttgaataa ttgatagcct
     1501 tttttctgtt gtgccgtcag cgcattctca tctccacgca accatttatc aaatggggaa
     1561 tccggcgtaa ttaatgtttt ctcaaattca gcaatggcat cagtaatatt ttcgccactg
     1621 aaaccttgcg gatagacttc aaggaactgc gctttaagct gcggatcttt ttccagctta
     1681 gcaataattt cgtcccagga tttcgacgcc atttcaatcg gattcaacgg cggtccacca
     1741 gcctgatcct gcaatgttgc tgcacgacca tcccagaact gctcaacgtt aaatactgag
     1801 ttaaataccg tcggcgcgtt aatcggcccc actgcgccac caacaccaat cgatgttttt
     1861 ctgccatcga cgccccccgc attcaacgca tggcaatgag cgcatgaaat ggtgctatcc
     1921 gccgataaac ggggatcgtg atacagcgca aagcccaacg ccactttttg cgcatcggta
     1981 ggcagttttt gcgggatggg ctgcaccggt tcattacgat gatccggtgc agtgtcattg
     2041 ctggcgtaat attccgcgcg ctgttttgca atccaggcga gtatttccgc ccgctcttca
     2101 tcactcacct tacccgccca gtgtagcgcg gtataacgcg ttggtggcat ggtttcatac
     2161 tgcatcaccc attcaatctt attcagatcg ctttgcgaaa cgggtttatc agccagcaga
     2221 gccgcacgca cggcctcgag gttaaaagat ttatatccaa gcttaatgtc gtaatccatc
     2281 aactgtttcg cgccaggaat ataataatag gcgggtaatt ctgccgaagg cgtgtggcaa
     2341 tagtcgcatc ctttttcgcg gagaaagcct aaaaccttat tattttcact gacagcagat
     2401 gcctgaacat cagcctgttt actgcgttta ttatcgtggt accacacata accagataac
     2461 cctaaatagc aaatcgcgac gccagccagg ccgatcgcgg taatacgtga gaccattttc
     2521 attattcttc cctcacggtg gtgagttatt gtgacccaga aaattttcca gaatgctgca
     2581 aacgtctctg cgagacaaag ccatcataaa aaacagggaa atatgctttt tgataggaat
     2641 caatcaatta catgaatata aactatcaat aagatagcct gtgcctatta cccagatcag
     2701 gaatggcata tcatgattgc ggcgagagca ggattgaatg ttctgaaaat gaaaaacaac
     2761 acgaaatctg tatttttata aattccatgg atattagtgc gttatatttt ttgacaggta
     2821 aataaacgtg atccaccgca cgctttgtcg cccaccaggc ggagcgaatg actaccctta
     2881 aagaaaagcc cgataattag caacgaattt cggaggttgg atccttatgc tcaatcagaa
     2941 aattcaaaac cctaatccag acgaactgat gatcgaggtc gatctctgct atgaactgga
     3001 cccgtatgaa ttaaaactgg atgagatgat cgaggcagaa ccggaacccg agatgattga
     3061 agggctgccc gcctctgatg cgctgacgcc tgctgatcgc tatctcgaac tgttcgagca
     3121 tgttcagtcg gcgaaaattt tccccgacag taaaaccttt cccgactgcg cacctaaaat
     3181 ggacccgctg gatattttaa tccgctaccg taaagtgcgc cgtcatcgtg attttgactt
     3241 gcgcaagttt gttgaaaacc acttctggct accggaggtc tactccagcg agtatgtatc
     3301 ggacccgcaa aattccctga aagagcatat cgaccagctg tggccggtgc taacccgcga
     3361 accacaggat cacattccgt ggtcttctct gctggcgctg ccgcagtcat atattgtccc
     3421 gggcggccgt tttagcgaaa cctactattg ggattcctat ttcaccatgc tggggctggc
     3481 ggaaagtggt cgggaagatt tgctgaaatg catggccgat aacttcgcct ggatgatcga
     3541 aaactacggt cacatcccca acggcaaccg cacctattat ttgagccgct cgcaaccacc
     3601 ggtttttgcg ctgatggtgg agttgtttga agaggatggt gtacgcggtg cgcgccgcta
     3661 tctcgaccac ctgaaaatgg aatatgcctt ctggatggac ggtgcagaat cgttgatccc
     3721 taatcaggcc tatcgccatg ttgtgcggat gccggacgga tcgctgctca accgttactg
     3781 ggacgatcgc gacacaccgc gtgacgaatc ctggcttgag gacgttgaaa ccgcgaaaca
     3841 ttctggtcgc ccgcccaacg aggtgtaccg cgatttacgc gcgggggcgg cctccggttg
     3901 ggattactct tcccgctggc tgcgtgatac tggtcgtctg gcgagcattc gtaccaccca
     3961 gttcatcccc atcgatctga atgccttcct gtttaaactg gagagcgcca tcgccaacat
     4021 ctcggcgctg aaaggcgaga aagagacaga agcactgttc cgccagaagg ccagtgcccg
     4081 tcgcgatgcg gtaaaccgtt acctctggga tgatgaaaac ggtatctacc gcgattacga
     4141 ctggcgacgc gaacaactgg cgctgttttc cgctgccgcc attgtgccgc tctatgtcgg
     4201 tatggcgaac catgaacagg ccgatcgtct ggcaaacgcc gtgcgcagtc ggttactgac
     4261 acctggcggg attctggcaa gcgagtacga aaccggtgaa cagtgggata aacctaacgg
     4321 ctgggcacca ttacaatgga tggcgattca gggatttaaa atgtacggcg atgaccttct
     4381 gggtgatgaa atcgcgcgca gctggctgaa gacggtgaat cagttctatc tggaacagca
     4441 caaactgatc gagaaatacc atattgccga tggtgttccc cgcgaaggcg gcggtggcga
     4501 gtatccgttg caggatgggt ttggctggac taacggtgtg gtgcgccgtt taattggttt
     4561 gtacggtgaa ccataatatt tttacagcca gccgctaact tcctgctggc tgtaaaatta
     4621 tcctcttcag gaggagatat ttaacatcat tgccgcctgg gtgcgatttt tcacttccag
     4681 acggcgatac agggattcca gatgcgcttt taccgttccg gtactgatat tcagcgccct
     4741 gccgatctcc ttatttgatt cgcccgccgc taacatggtt aaaatctccc gctggcgggc
     4801 gcttaacgat ttgagatctt taatgtcctt ttccggcgtc gtccgccagt ctccaggcag
     4861 aaacatcatc cccatcgccg cactatttac cgccaacgca aatgtctcga cggttgaatc
     4921 acgaggcaca atggccagca cattaaaatg gataacttcc tgtaaccacc gtttattgca
     4981 atccgtcgcc gtaattaaca ccttaacctc aggaaattgc accacggttt tttgcagtaa
     5041 ccagtagcaa aactcaccat cctgatcgcc atcgagcatc actaaggctt cagggtaact
     5101 ttccagcttt tgccataact catctgcctg actggccccc tgaatactca ctcctggaat
     5161 acgctgctgt aaactgattt tcattccatg aataaatatt gactgcctgt caaacatgac
     5221 tatttgcata actgaatctc cacctgaata cgttaaaaag acttaagtag tggaagggta
     5281 ttacccgcga gaaaaaataa gaattcgcca tttggcggtg gccattctac agagatgacg
     5341 cgtagaaaat agttaccgat ataaatcgtt acagctaaac gcctgaaatt acatgtcgag
     5401 ggcactattt aaaacaattt tgaggatttc cttatattga tggttagtac gcatgcaatt
     5461 aaaaatgaaa ttccgcgacc acaagcaaaa ataacaaatg gcaatgagac aaaaataagc
     5521 acaaatagcc aacacgtcct ctgttcactt taaagggaat cgctgaaaaa aaacgctctg
     5581 tttagggaaa ttcacctttc tcataaagct attccgccct tttcctgctg agaaatcgcc
     5641 acattcgtca tgacaacatt gtgaaacccg gcattagatg ttagaaaaaa caataaacaa
     5701 tgcgatatgc gcgttactgt ttcgctgtga acaacaatcg gtcaaagaaa tggataaaat
     5761 tcacgcaatg cagttgttca tcaaagtcgc ggagctggaa agtttttccc gcgcggcgga
     5821 tttctttgct ttgccaaagg gaagtgtttc gcgccagata caggcactgg aacatcaact
     5881 tggcacccag cttctccagc gcaccacgcg gcgggtcaaa ctcacgccag aaggcatgac
     5941 ctattatcaa cgggcaaaag atgtgttgag taatctcaac gaactggacg gtctgtttca
     6001 acaggatgcc accagtatca gcggtaaatt acgcatcgac atcccgccag gaatcgcgaa
     6061 aagcctgtta ctgccgcgtc tgtcggagtt tctctatctg catccgggaa ttgagctgga
     6121 gctgagtagc catgaccgtc cggtagatat tcttcacgat ggttttgatt gcgtgatacg
     6181 cactggcgcg tcaccggaag atggcgttat cgcccgtccc ctcggcaaac tgaccgtggt
     6241 caattgtgcc agtccacact atctgacgcg ctttggttat cctcaaagcc ccgacgatct
     6301 gacttcgcac gcaatagtgc gttacacacc gcacctgggt gtacatccgt taggttttga
     6361 ggttgccagc gttaatggcg tccagtggtt taagtctggc ggcatgttga cggtaaacag
     6421 tcgcgaaaac tatctcgccg ccggtcttgc cggtctgggg attattcaga tcccgcgcat
     6481 tgccgtgcgc gaagccctgc gtgccgggag gcttattgaa gtattacctg gctaccgtgc
     6541 cgagccgctc tccctttcgc tgatttatcc gcagcgtcgg gagctttccc ggcgtgtaaa
     6601 cctgtttatg cagtggctgg ctggcgtaat gaaagagcac ctggactgac cgactatact
     6661 ttttaagaat gaccacgaca gaaggacaaa agagcggatg acgcaggaaa acgagatcaa
     6721 acgtcccacc caggatctgg agcacgagcc gattaagcag ttggataata gcgagaaagg
     6781 cggcaaagtt agccaggcgc tggaaaccgt caccactact gccgagaaag tccaacgcca
     6841 accggttatt gcgcacctga ttcgcgcaac agagcgcttt aacgatcggc tgggtaacca
     6901 gtttggtgcg gctatcacct atttctcatt tttgtcgatg atcccgattt tgatggtgtc
     6961 gtttgccgca gggggctttg tgctggcctc ccatccgatg ttgctacagg atatcttcga
     7021 caaaattctg caaaacatca gcgatccgac gctagccgcc acgttgaaaa acaccatcaa
     7081 caccgccgtt cagcagcgta ctaccgtagg gcttgtcggc ctggcggtgg cgctttattc
     7141 cggcatcaac tggatgggta acctgcgtga agcgattcgc gcccagtcgc gcgatgtctg
     7201 ggaacgctcg ccgcaagatc aggagaagtt ctgggtaaaa tatctgcgtg attttatttc
     7261 gctgattggt ttgttgattg cactgattgt gacgctttcg atcacctcgg ttgccggttc
     7321 ggcgcagcaa atgattatta gcgccctgca cctgaacagc attgagtggc tgaaaccgac
     7381 gtggcggttg attggcctgg cgatttccat cttcgccaac tatctgcttt tcttttggat
     7441 cttctggcgg ttgccgcgcc accgtccgcg caaaaaagcc ctgattcgtg gaacatttct
     7501 ggctgcgatt ggttttgaag tgattaaaat cgtgatgacc tacaccctgc catcgctgat
     7561 gaaatccccc tctggcgcag catttggttc cgtgctgggg ctgatggcgt ttttctactt
     7621 cttcgcccgt ttgacgctgt tttgcgcggc gtggattgcc accgccgaat ataaagacga
     7681 cccgcgaatg ccggggaaaa cgcagcggta attcattggt ttataatgcc tgataaacgc
     7741 acggtcgatc ccctcgcccc ttcggggaga ggattagggt gagggggtac aagccagcca
     7801 gagaccaggc aacgacatgc acataaccac ctgaaactac atctcttcag gagcaaacaa
     7861 tttcatacca actcataacc ccagcatata aatccagttg gtaactttta tttaacctga
     7921 aaccagt
//
LOCUS       DACBVI010000183         7920 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.184, whole genome shotgun sequence.
ACCESSION   DACBVI010000183 DACBVI010000000
VERSION     DACBVI010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.184"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            128..925
                     /locus_tag="IU325_20120"
     CDS             128..925
                     /locus_tag="IU325_20120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-181"
                     /protein_id="HAP5811309.1"
                     /translation="MRVLALSAVFLVASIIGMPAVAKEWQENKSWNAHFTEHKSQGVV
                     VLWNENKQQGFTNNLKRANQAFLPASTFKIPNSLIALDLGVVKDEHQVFKWDGQTRDI
                     AAWNRDHDLITAMKYSVVPVYQEFARQIGEARMSKMLHAFDYGNEDISGNVDSFWLDG
                     GIRISATQQIAFLRKLYHNKLHVSERSQRIVKQAMLTEANGDYIIRAKTGYSTRIEPK
                     IGWWVGWVELDDNVWFFAMNMDMPTSDGLGLRQAITKEVLKQEKIIP"
     gene            complement(1332..1484)
                     /locus_tag="IU325_20125"
                     /pseudo
     CDS             complement(1332..1484)
                     /locus_tag="IU325_20125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
     gene            1860..2825
                     /locus_tag="IU325_20130"
     CDS             1860..2825
                     /locus_tag="IU325_20130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicase"
                     /protein_id="HAP5811310.1"
                     /translation="MRKCAQLELFRERLPRKPYYSDELTTGLRIADVARALGARYIQP
                     NGPTHRHWIVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDG
                     NAAPLRYAAAIEAALREKLGADMGYSGLICKNPLHEHWLVQVWEPRLYDLAWLSDYLD
                     LSSYNGRKSLPEYGLGRNCTLFEKTRLWAYKAIRQGWPDYPEWLAACVDRASGYNAQF
                     EQPLPANEVRHTAKSIAKWTHQHLSPAGFREEQARRGAKGGKVSKGGGRPSNSGKDKS
                     DLLPEVLRLKAQGYTNRDIADDLGEHKKYWGSVSYAAIAGPFPSL"
     gene            complement(2789..4180)
                     /locus_tag="IU325_20135"
     CDS             complement(2789..4180)
                     /locus_tag="IU325_20135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4-like element ISKpn19 family transposase"
                     /protein_id="HAP5811311.1"
                     /translation="MQLTLQIVITDESGSSRTEELMTIQKSGETRNDIGLSVSESKLL
                     LNTVQQSVVQLQADEYTQHHIRCPHCLAARRIKGKQKIRYRTLFGVIPVSGLRVYRCR
                     CEESDTKTVSLLSDWAGDYSHPALKYIETRWASMISYEMTTRLLKDILPVGHSLNAST
                     VRNHLCQVAQRLDAEAEAHSGFLSGCPRDWGNLPRPGKPLVVGIDGGYVRDRDDKKRN
                     FEIIAGKSFSVGAPADTRRFGFVQKDDCHPERRLMTHLSAQGMQANQQIFFLSDGADN
                     LRDLQFGMYPESTHVLDWFHITMRLTVLMQYARGLLVSDPEAGSKVLALLESIKRYLW
                     HGNVVAALEHIDNCVMYCDDPELSYPSLKSLQKHLDEMYTYIRNNKMMIPNYGEMRRY
                     GEPVSTAFVESTINEVIARRMAKKQQMQWSRKGAHYLLQTRTAVLNNELQDKFVCWYP
                     GFQSDGKGPAMAA"
     gene            complement(4217..4789)
                     /locus_tag="IU325_20140"
     CDS             complement(4217..4789)
                     /locus_tag="IU325_20140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP5811312.1"
                     /translation="MQYAYIRVSSADQNTARQEEALSKAGFQPDKICVEHASAKDTNR
                     PGLQELLGQLRPGDTLLVHSIDRLCRNMSDMCAVTTRLRDQGVTLIFLKEQLTFSAGT
                     NNPMQELQLHMMSAFSQFERALLKERQADGIAAKKARGEKTGRPGADIKKIQEIDALR
                     SRGVRLRIACDHAGLGVSTYYKLRHQMNNQ"
     gene            complement(4926..5516)
                     /locus_tag="IU325_20145"
     CDS             complement(4926..5516)
                     /locus_tag="IU325_20145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HAP5811313.1"
                     /translation="MKIYVIKIAVHGVSPMVWRRLRIAADTSLAALHFIFQIVQGWGD
                     DHLHQFHIYGKDYGISYEGGIGFVDNPFRVVIDDFAFDAGDRFTYEYNFFEHWLHDIR
                     VEAIYENSTLKAPFCISGHGMPGATAADEFDKTLAFLEAIVNADDETTVGEIRPFADD
                     LDAVRFNRHKINRQLSRLDLASPVLEPEVIWLGRRR"
     gene            complement(5607..5789)
                     /locus_tag="IU325_20150"
                     /pseudo
     CDS             complement(5607..5789)
                     /locus_tag="IU325_20150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060658.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
     gene            5951..6565
                     /locus_tag="IU325_20155"
     CDS             5951..6565
                     /locus_tag="IU325_20155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014754501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP5811314.1"
                     /translation="MFIGYIRMSKADGSQTNDLQRDALIAAGVDAAHFYEDRASGKRE
                     ERPGLTACLKALRPGDTLVVWKLDRLGRDLRHLINTVHDLTARGTGLKVLTGHGATID
                     TTTAAGKLVFGIFAALAEFERELIAERTTAGLASARARGRNGGRPYKMTPVKLRLAMA
                     SMGQSETKVSTLCQELGITRQTLYRHISPVGQLRADGIKLFNRG"
     gene            complement(6884..7540)
                     /locus_tag="IU325_20160"
     CDS             complement(6884..7540)
                     /locus_tag="IU325_20160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrS1"
                     /protein_id="HAP5811315.1"
                     /translation="METYNHTYRHHNFSHKDLSDLTFTACTFIRSDFRRANLRDTTFV
                     NCKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIEFRACDLKGANFSRT
                     NFAHQVSNRMYFCSAFISGCNLSYANMERVCLEKCELFENRWIGTNLAGASLKESDLS
                     RGVFSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIAAWQQELILEALGIVVYPD
                     "
BASE COUNT         2035 a         1931 c         1978 g         1976 t
ORIGIN      
        1 aatagttaaa gcttgtgcct gattgttgcc acaattggtc agcagtttgc tagggaatga
       61 gagggcagag aatgataagc ttagcactta atttgtatgg atatattgca ttaagcaagg
      121 ggacgttatg cgtgtattag ccttatcggc tgtgtttttg gtggcatcga ttatcggaat
      181 gccagcggta gcaaaggaat ggcaagaaaa caaaagttgg aatgctcact ttactgaaca
      241 taaatcacag ggcgtagttg tgctctggaa tgagaataag cagcaaggat ttaccaataa
      301 tcttaaacgg gcgaaccaag catttttacc cgcatctacc tttaaaattc ccaatagctt
      361 gatcgccctc gatttgggcg tggttaagga tgaacaccaa gtctttaagt gggatggaca
      421 gacgcgtgat atcgccgctt ggaatcgtga ccatgactta attaccgcga tgaagtactc
      481 agttgtgcct gtttatcaag aatttgcccg ccaaattggt gaggcacgta tgagtaaaat
      541 gctgcacgcc ttcgattatg gcaatgagga tatctcgggc aatgtagaca gtttttggct
      601 cgatggtggt attcgcattt cggctaccca gcaaatcgct tttttacgca agctgtatca
      661 caacaagctg cacgtttctg agcgtagtca gcgcatcgtg aaacaagcca tgctgaccga
      721 agccaatggc gactatatta ttcgggctaa aacgggatac tcgactagaa tcgaacctaa
      781 gattggctgg tgggttggtt gggttgaact tgatgataat gtgtggtttt ttgcgatgaa
      841 tatggatatg cccacatcgg atggtttagg gctgcgccaa gccatcacaa aagaagtgct
      901 caaacaggag aaaattattc cctagaagtg gttagcgcgt atttgtgtga aatagccgtc
      961 atataagctg taaagatata tggacaaaat gctgatgctc gatgcgcttg tagttaatgg
     1021 ctcatactta atcggtcagg cctaatcgac ttaagctccg cgcgttaact ttcagcctga
     1081 gcattagcat cacattccta aacgtaaaaa gcctagccaa tttgctaggc ttttttgtta
     1141 tctcaatggc catggtcact caacatgggg gtgtgaataa aggagtggcg atgtcagtaa
     1201 aggtgaatgc cgctgcgata aagtgcctag cccatcaagg attgtaggct ttcttggctg
     1261 ctaaagcgtt tgacgaaaaa gtccaagaag gcgctgatat tggttctcca tcattttcat
     1321 ctaccggttt actataggtc aaccaggccg tccttgccgg cgcttgggac gcagacgatg
     1381 gcatcaaatg cctcgctcaa attcgtttct acagaggcgc tttgggaccg cattcgcttt
     1441 gctttcatgg gggcatctgt caatgtcaga catgaatcct ccgatatagg ttgatttttg
     1501 atctcttcaa cacttggtgt tgtccgacta gagagactta tatggatcag tattcttgca
     1561 ctgacaatgg tgccactcaa tcccaaggat cgacctagtt agaggcgtag atcaagcaat
     1621 tcaaaacagc gcagaagcag aaggaggtgg aaatatgttc cagggtgaaa ggttacatgc
     1681 aactaacgtt gctctctagg atgtgcctgc tgtaacgtat tgtccagaaa agacaaaccc
     1741 ccgttatcgc ttgcatcttg gcggagctgc gaaaaacggg ggccgtgacg aatcacaaga
     1801 gaagtctatc acatggatca tgccaaacaa ccccacgccg ccaataagcg cggtggtgct
     1861 tgagaaagtg cgcacagctt gagctgtttc gggagcgcct gccccggaaa ccctactatt
     1921 ccgacgagct tacaacgggc ctacggattg cggatgttgc ccgcgccctt ggtgcgcgtt
     1981 acatccagcc gaacgggcca acgcaccgcc actggatcgt ttttgacgta gaccatgcag
     2041 ccgctacgtt atcttgggat gacgtaggcg ctccagcccc taacattacc gtcacgaaca
     2101 aggccaacgg ccacgcgcat ctgatctacg gactggatac gccgatccgc actgccccgg
     2161 atggaaatgc agcccctctg cggtatgccg ccgccatcga ggccgcgctc cgggagaaat
     2221 taggggcgga tatgggctac tccgggctga tctgcaagaa ccccctgcat gagcactggc
     2281 tggtgcaggt atgggaaccc cggctctacg atttagcctg gctatccgat tacctcgatc
     2341 tgagctccta caatggccgc aaatcgcttc ctgagtacgg gcttgggcga aactgtaccc
     2401 tgttcgagaa aacgcgcctg tgggcctata aagcgatccg gcagggctgg ccggattacc
     2461 ccgaatggtt ggccgcttgt gttgaccggg catcaggata taacgcccag ttcgagcagc
     2521 cgctccccgc aaacgaagtc cggcacacgg ccaagtccat tgccaaatgg acgcaccagc
     2581 acctaagccc cgcagggttc agagaggagc aggcccggag aggtgcgaag ggcggcaagg
     2641 tatccaaggg agggggaagg ccgagcaata gcggtaagga caagtccgat ctattgccgg
     2701 aggttctgcg gcttaaggct caggggtaca ccaaccgcga tatagcggac gatcttggag
     2761 agcataaaaa atattggggc agcgtgagct atgctgccat cgctggtccc ttcccgtcac
     2821 tttgaaaacc cggataccag cagacaaatt tatcctgcag ttcgttattc aaaacggcgg
     2881 tccgggtctg taacaaataa tgagcccctt ttctgctcca ctgcatctgc tgctttttgg
     2941 ccattcgtct ggcgatcact tcattgatcg tggattccac aaacgcggtt gataccggct
     3001 ctccgtaccg acgcatttcg ccgtagtttg ggatcatcat cttattattc cggatgtagg
     3061 tatacatttc atccagatgt ttttgcaggg atttcaggct aggatagctg agttcagggt
     3121 catcgcaata cataacgcaa ttatcaatat gttccagcgc agcgacaaca ttgccatgcc
     3181 agagatatcg tttaatgctt tccagcagtg ccagaacttt actccccgcc tctggatctg
     3241 ataccagcag tccccgggca tattgcatga gcaccgtcag cctcatggtg atatgaaacc
     3301 agtccagcac atgcgttgac tcagggtaca taccgaactg gaggtccctg agattatccg
     3361 cgccgtcgga cagaaaaaat atctgctggt tggcctgcat tccctgcgct gaaaggtggg
     3421 tcatgagcct gcgttccgga tgacagtcat ccttctgaac aaaaccgaag cggcgggtgt
     3481 cagcaggcgc accaacggaa aaagacttcc cggcaataat ttcaaaatta cgctttttgt
     3541 catcccgatc gcgaacataa ccaccatcta tacccaccac aagcggtttt cctggcctgg
     3601 gcaggttccc ccagtcgcgg gggcagccgg aaagaaaacc tgaatgagct tcagcctcag
     3661 cgtcaagacg ctgcgccacc tggcataaat gattcctcac cgttgaggca ttcaggctgt
     3721 ggcccaccgg cagaatatct ttcagcagac gagtcgtcat ttcataggag atcatcgagg
     3781 cccagcgggt ttcaatatat ttcagtgccg ggtgagaata atcgccggcc cagtcgctga
     3841 gcaaactgac tgtctttgta tcactctctt cacaacggca ccggtacacc cgaagcccgg
     3901 ataccggaat aacgccaaac agcgtccggt accgtatttt ctgtttgcct ttgattctgc
     3961 gcgcagcaag gcagtgaggg caccggatat ggtgttgagt atattcgtct gcctgcagct
     4021 ggaccaccga ctgctggacc gtattcagca acagcttgga ttctgacacc gataatccga
     4081 tgtcgttccg ggtctcccct gatttctgga ttgtcatcag ttcctccgtt cgactggagc
     4141 ctgattcatc ggtgatgact atctggagcg tcagttgcat cattgcctcc tgctgtggtg
     4201 gatgactatc tcccggctac tggttgttca tctggtgacg aagcttataa tatgtggaaa
     4261 cgccaaggcc cgcatggtcg caggcgatcc tgagcctgac ccctctgctt ctgagtgcgt
     4321 caatttcctg gatctttttg atatcggcgc cggggcgccc tgttttttca ccccgtgctt
     4381 ttttcgcggc gatgccgtcg gcctgccttt ccttcagtaa ggctcgttca aactggctga
     4441 atgccgacat catgtgcagc tgcagttcct gcatcggatt gtttgtgccg gcgctgaacg
     4501 tcagctgctc tttcagaaaa ataagggtaa cgccctggtc acgaagccgg gtggtgacag
     4561 cgcacatatc cgacatattc cggcaaaggc gatcgatgga atgaaccaga agcgtatcac
     4621 cagggcgtaa ttgccccagg agttcctgta accccgggcg gtttgtgtcc ttcgcgctgg
     4681 catgctcaac acaaatttta tcaggctgaa aacccgcctt tgacaatgcc tcttcctgac
     4741 gggccgtatt ctgatcggcc gagcttaccc tgatgtaggc atattgcacg caaaacctcc
     4801 ggaaggtctt taaacatcat agagatttaa agaataatcc ataaacactg atttagggag
     4861 gtttatagac attttttcgg ggatctgaga ggcatgctga aaacgtatac ctttatagac
     4921 atcagtcagc gacgacggcc cagccagata acttcaggct ccagtaccgg agatgcaagg
     4981 tcgagcctgc tcagttgccg gttgattttg tggcggttga acctgacagc atccaggtcg
     5041 tcagcaaaag ggcggatctc gccgaccgtt gtttcatcat ccgcattaac gatagcttca
     5101 agaaacgcca gggttttatc gaactcatcc gcagctgtgg ctccgggcat accatggccg
     5161 cttatacaaa acggcgcttt cagcgtagag ttttcataaa tggcctcaac acgaatgtca
     5221 tgaagccagt gctcaaagaa attgtattca taggtaaagc gatcgccagc atcaaaagca
     5281 aaatcatcaa tcacgacccg gaacggatta tcgacaaaac cgatgcctcc ttcgtaggag
     5341 ataccgtaat ctttgccata aatgtgaaat tgatggagat ggtcgtcccc ccagccctgc
     5401 actatctgga aaatgaagtg aagcgcggcc agtgacgtgt cggcagcaat tctcagccga
     5461 cgccagacca tcggactgac tccatgaact gctattttga tgacgtagat cttcatagtc
     5521 agcaggtatt gttatgaaat gatggtggtt attttaaccc ctgtagatgg catcattcga
     5581 tggctgcccc gaggtttttc atgctctccg atctggatga tgcaataaat ttgagcgtga
     5641 agcccccggc gactgcaggc cagcgccagt tcatattcat tcagcgccag aaactccagc
     5701 cgctgtgcat catcgaaatc aggtagacca tagagcgctt cctgctcggc gtccgaaaga
     5761 atactgagga gcttattttt agtaggcatc tctgatcctt atgcctattt aaacgcgctt
     5821 gtcacattat cattaacata gaacctttac gtgtcataat ttaaattacg ggtaatgtga
     5881 ctaccgttga cagattatgg tacccaaatc agaatgtcac ataatcgaac gtatatgtga
     5941 cagaggaaat atgttcatcg gatatattcg gatgtcaaaa gcggatggtt cgcagacgaa
     6001 tgatttacaa cgtgatgcac tgatcgccgc aggtgttgat gccgcacatt tttatgagga
     6061 ccgggcatcg ggcaaacgag aagagcgtcc tggacttaca gcctgtctga aagcactacg
     6121 acccggagac acgctggtcg tatggaagct ggaccgcctc ggtcgtgatc ttcgtcatct
     6181 cattaataca gtgcacgacc tgaccgcgag ggggaccggt ctgaaagtgc tgaccggtca
     6241 cggggcgact atcgacacaa caacggccgc tggcaagctt gtcttcggta tctttgccgc
     6301 actggcggag tttgagcgcg aactgattgc tgaaagaacg actgcaggtc tggcatcagc
     6361 aagagcccgt gggcggaacg gcggccgacc ttataaaatg acgccagtca aactgcgact
     6421 ggcaatggca tcaatgggac aatcagaaac aaaggtcagt acgctatgcc aggaacttgg
     6481 gataacccgg caaactttgt acaggcatat ttcccctgtt ggtcaactgc gggcggacgg
     6541 gatcaagctg ttcaaccgtg gataatttga acggacggaa aaatctgggg ttccggcgta
     6601 gtacccctat cacctccaca tttttgcaac agtgccttat aaatggcagc cttatgttta
     6661 cttgccaaga tgggctacca tatgatgttt tatactgagc aatattaata ttatctttaa
     6721 tggaaaaatt aagcccacta agtgacccac tgctatatgg ctctattgtg aagacgcctg
     6781 agggtaaaaa tttatacatg gttgtcccta tgtctattat tgcaaggttg acaatattat
     6841 tcgttttata aatttgagcg tttaaaatca cacatcaaag caattagtca ggataaacaa
     6901 caatacccag tgcttcgaga atcagttctt gctgccaggc tgcaattttg atacctgatg
     6961 tatcgacttt gcggggatct aaaccgtcga gttcggcgtg gcataaattg gcaccctgta
     7021 ggctaaattg cccccagaca tcttcggaaa aaacacctcg acttaagtct gactctttca
     7081 gtgatgcacc cgctaggttc gttcctatcc agcgattttc aaacaactca catttttcta
     7141 aacaaaccct ctccatattg gcataggaaa gattacatcc agaaataaat gctgagcaaa
     7201 agtacatacg attactcact tgatgggcaa agtttgttcg ggaaaagttg gcacctttta
     7261 aatcacacgc acggaactct ataccgtagc aattggcatt actgaagttt gccatcgcaa
     7321 gttggcattg ttggaaactt gcatcacgaa gatctgcgac atcaaagtgg cagccttcga
     7381 tatcaccctg ttcaatgaac ttgcagttga cgaatgtcgt atcacgcaag ttagcacgtc
     7441 gaaagtcgct gcgaatgaat gtgcaagcgg tgaaggtgag atcacttaag tctttatgtg
     7501 aaaagttgtg gtgccgatat gtatgattgt aggtttccat gagtactcct tgttgaaagg
     7561 agggctagac taccattata catatgccaa gacattaaaa taaaagcctg ttacatcaac
     7621 acattacaac ccctcaattt acctgtttta agtggtcaaa ttggcgcgta gagcgcctat
     7681 cgctcttgca ttggcacgcg gccctgttga aaaaagttat tcgctttaga tagggttagg
     7741 agagccgtgc ttcgccggta aaaaatcgga ctatccgacg cagggcggac tcatcggcgg
     7801 agaagcaaac tttgactata tctccctaca cttggaagag ggctagactg gccccataga
     7861 tcaccagaca cttcgactct cttaaaataa gaggtagtct tggaactatg tttaatacca
//
LOCUS       DACBVI010000184         7913 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.185, whole genome shotgun sequence.
ACCESSION   DACBVI010000184 DACBVI010000000
VERSION     DACBVI010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.185"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            20..1786
                     /gene="adeD"
                     /locus_tag="IU325_20165"
     CDS             20..1786
                     /gene="adeD"
                     /locus_tag="IU325_20165"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP5811316.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYTDAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNC
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(1833..3224)
                     /gene="uhpT"
                     /locus_tag="IU325_20170"
     CDS             complement(1833..3224)
                     /gene="uhpT"
                     /locus_tag="IU325_20170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HAP5811317.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3480..4799)
                     /gene="uhpC"
                     /locus_tag="IU325_20175"
     CDS             complement(3480..4799)
                     /gene="uhpC"
                     /locus_tag="IU325_20175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HAP5811318.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4809..6311)
                     /gene="uhpB"
                     /locus_tag="IU325_20180"
     CDS             complement(4809..6311)
                     /gene="uhpB"
                     /locus_tag="IU325_20180"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HAP5811319.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNIWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHSVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(6311..6901)
                     /gene="uhpA"
                     /locus_tag="IU325_20185"
     CDS             complement(6311..6901)
                     /gene="uhpA"
                     /locus_tag="IU325_20185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HAP5811320.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFSSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
BASE COUNT         2067 a         2189 c         1947 g         1710 t
ORIGIN      
        1 aaatcaaaat gagaaaaata tgaataattc tattaaccat aaatttcatc acattagccg
       61 ggcggaatac caggaattgt tagccgtttc ccgtggcgac gctgttgccg attatattat
      121 tgataatgtc tctattctcg acctgatcaa tggcggagaa atttccggcc caattgtgat
      181 taaaggacgt tacattgctg gtgttggcgc agaatacact gatgctccgg ctttgcagcg
      241 gattgatgcc cgcggcgcaa cggcggtgcc agggtttatt gatgctcacc tgcatattga
      301 atccagcatg atgacgccgg tcacctttga aaccgctacc ctgccgcgcg gcctgacaac
      361 tgttatttgc gaccctcatg aaatcgtcaa cgtgatgggc gaagccggat tcgcctggtt
      421 tgcccgctgt gccgaacagg caaggcaaaa ccagtactta caggtcagct cttgcgtacc
      481 cgccctggaa ggctgcgatg ttaacggtgc cagttttacc cttgaacaga tgctcgcctg
      541 gcgggaccat ccgcaggtta ccggccttgc agaaatgatg gactaccctg gcgtaattag
      601 cgggcagaat gcgctgctcg ataaactgga tgcatttcgc cacctgacgc tggacggtca
      661 ctgcccgggt ttgggtggta aagaacttaa cgcctatatt gctgcgggta ttgaaaactg
      721 ccacgaaagt tatcagctgg aagaaggacg ccggaaatta caactcggca tgtcgttgat
      781 gatccgcgaa gggtccgctg cccgcaatct caacgcactg gcaccgttga tcaacgaatt
      841 taacagcccg caatgcatgc tctgtactga tgaccgtaac ccgtgggaga tcgcccatga
      901 aggacacatc gatgccttaa ttcgccgcct gatcgaacaa cacaatgtgc cgctgcatgt
      961 ggcatatcgc gtcgccagct ggtcgacggc gcgccacttt ggtctgaatc acctgggctt
     1021 actggcaccc ggcaagcagg ccgatatcgt cctgttgagc gatgcgcgta aggtcacggt
     1081 gcagcaggta ctggtgaaag gcgaaccgat tgatgcgcaa accttacaag cggaagagtc
     1141 ggcgagactg gcacaatccg ctccgccata tggcaatacc attgcccgcc agccagtttc
     1201 cgccagcgac tttgccctgc aattcacgcc cggaaaacgc tatcgggtaa ttgacgtcat
     1261 ccataacgaa ttgattaccc actcccgctc cagcgtctac agcgaaaatg gttttgatcg
     1321 cgatgatgtg tgctttattg ccgtacttga gcgttacggg caacggctgg ctccggcctg
     1381 tggtttgctc ggcggctttg gcctgaatga aggtgcgctg gctgcgacgg tcagccatga
     1441 cagccataat attgtggtga tcggtcgcag tgccgaagag atggcgctgg cggtcaatca
     1501 ggtgattcag gatggcggcg ggttgtgcgt ggtacgtaac tgccaggtcc aaagccatct
     1561 gccgttaccc attgccgggc tgatgagcac cgacacggcg cagtcgctgg cggaacaaat
     1621 tgacgccttg aaagctgccg cccgtgaatg cggtccgtta cccgatgagc cgtttattca
     1681 gatggcgttt ctttctctgc cagtgatccc cgcgctgaaa ctaaccagtc aggggctatt
     1741 tgatggcgag aagtttgcct tcaccacgct ggaagtcact gaataataaa aaaaagcccg
     1801 gcgtcatgcc gggcaaaagt caccagttac gtttatgcca ctgtcaactg ctgaattttt
     1861 ttctcgcggc ggattttgcg ttcttccatc accgccacta tcgccatcag gcagatacaa
     1921 ccaatcgcgg cgatatcaag tgcggcgaag gtgcctgccc agccggtaag gccgaatacc
     1981 ggcgtcccat cggcaatcat tcccagacct aacttggcaa agctgtcacc aatcaggtaa
     2041 gcgaaggtgc ctttaatacc atcggcagcg ccaatcgctt ttttaggtac aaagccaaca
     2101 gcagccacac caatcaacaa ttgcgggcca aagaccagga aacccaacgc aaagagagaa
     2161 gccaggtaga tatattcgtt gctggcatgt tgatacacac cgagcgtggc gataatcagc
     2221 gccagcgcga tgcaggccac caggccacgg cgaccgttcg ccaggtcaga gagccagccc
     2281 cacagcagcg taccgaccag cgcaccagct tcaaacagcg taaagccctg aatcgccacc
     2341 gctttagaga gtttcagttc ctggaacgca tacacggttg accactggtc gataccaata
     2401 cgcaccacat agaggaaaat gttggcgaag cacagcagcc agatcacttt gtttttcagc
     2461 acatactcaa caaagatctg ccacttggtc atatcggtag attctgtctc tttatcctct
     2521 tcactgatct cctcgccaaa cagctcttca gctttgccga ggccataaga ttccggggag
     2581 tcgctgccgt aacgcaggcc gataaaacca acaatcagcg cgataatcga cgggaagata
     2641 aacatgccga taacatgacc atcgaacagg taatttgcgc cgaacagcgc cacacctgct
     2701 gcgcctgcac cgccaaggtt gtgagaaatg ttccagaaac cgaggaacgt cccgcgttta
     2761 cgacgcggcg tccatttggt gatggtggag taactgcacg aacccccggt actctggaaa
     2821 aagccactta aggcgtagaa ggcaatcatc aggaacaggc taaccgagcc gctgcccata
     2881 ctggcgctaa agcccagcat acaaatggca gacaggatca gcatgaacgg caggaattgt
     2941 ttggtgtttt tgccgtcggc gtagtaggaa accagcgttt tacccacgcc ataagtgatg
     3001 gagaaaccca ggccgatcat ccccagctgc gtcatgctca acccgtaggt cgaaatcata
     3061 tcgttctgcg cgatgttaaa gttcttgcga atcaggtaca tcgtcaggta gccgataaag
     3121 accaccaggt aggattgcat gaacggtttg aaccacattt tgcgccgcac ttcgagcgga
     3181 aggtccaggg tcggcttgcg gacctggttt aagaaagcca gcatgggtta ctcctgattt
     3241 cccttcatac ttcaagccgc aggtgcctta gctgcacgca ttcctcccgg tcacatagtt
     3301 cactatgctc ccgggactca cgcgtttgct gcgttcctgc aacttgaatt attttgggaa
     3361 agaattaatg aatgatggca ttgtaaaaat cagcagcaaa tcaggcctga gatagcgtcc
     3421 aggcaaaacc tggaaaattt cttagttttg ccgggtatga agtgaaaagg tgagatgcat
     3481 cacgcttctc gcggtgtctg ggcgttcaaa aagggcagta acagcagtgc ggaaatcccg
     3541 gcggcgatag agataaccac aaaaaatccg ctccagtgcc aggtatcgag tactttcgcc
     3601 agtggccaac cagcaagcga cgcccccaga taagcaaaca agccgacaaa ccccgtcgcc
     3661 gcccctgccg cctctttgtg ggaacattct gccgccgcca tgccgattaa catctgcggg
     3721 ccaaaaacaa aaaaaccaat ggtgaagaag caggttgcct gcatcacgta gctggcaaat
     3781 ggcatcagcc acagcgagcc gactgaaagc aaaattccgg cggcgaaaat caaattcatc
     3841 ggcccgcggt tgccgttaaa caatttgtcc gagccccaac ccgctaccag cgcaccgata
     3901 aatccgccca gttcaaacat tgtcactgcc gtattcgccg tgaccagatc gacgcccagc
     3961 gtctcggaca tatacaaatt gccccagtcg ttgattgccg cccggaccac atagaccagc
     4021 acatagcaaa acgaaagcag ccagatatac ggattcaaca acacatactt ggtgaggatc
     4081 tctttacgcg tcagccctgc cccttcttgt tgttgagcaa tctccagcgc gtcgtgtcgc
     4141 cattcaccaa ccgcaggtaa acctaacgcc tgcgggcgat cgcgcagccg ccagcagaga
     4201 aaaatcccca cgacgatcgc catacaacca gcaatcatca tcccggcacg ccagccgtaa
     4261 tgcagcgcag cagctgccat cacaatggga atgagcgcac caccaacgtt atgcgccgtg
     4321 ttccataatg cccaccaacc accgcgctcg gtacgagaat accaggccgt taacagacgc
     4381 gcacacaccg gtgaacccca gccctggaaa aaggcgttca gcacccagag cacggcaaac
     4441 gcccatagcg acgtcgagaa gccaaacaga atgttgatga tgcccgtggc gataagccct
     4501 atccccataa aataacgggc atttgagcga tcgctgacaa tgccggagac aaacttcgac
     4561 acgccgtagg taatgtaaaa cagggtcgct aacaggccga tatcgctacg gctgagcacg
     4621 ccattagcaa ggatttctgg tacggcggcg ttaaaacttt tccgcgtgaa gtaaaacagc
     4681 gcgtaaccca gccagatggt cagcagaata tgccgacgcc agtagcgata acgggcatca
     4741 atttcatgtt tatcagtcat taatggcgca tcggcaggcg ctttcagaaa cggcaacatc
     4801 atcaaacctt agacatagcg ttgaggtaga gaaacgctga cacgcgtgcc gtgcagacag
     4861 gaaatggtca aggtgccgcc cagcgccgtt acgcgctcgc gcattccggt gaggccaaaa
     4921 ccttgttgcc cggaacccgg cggcaaaccg ctgccatcgt cttcaatcac cagcatcaac
     4981 cgttcatcct gctgccagcc ttgcagggtg accgcgctgg catcggcatg tttcacaatg
     5041 ttgttcagcc cttcctggca gacgcgaaac agcgtcactc gctggttttc acttaacgct
     5101 gattcatcga ttcgccattc gagatggctg acaatgccgc gcccttccag ctccatttcc
     5161 cgcatcagtg agcggatggc ctgctccagg gtgagatcat ccaactggcg cggacgtaac
     5221 cgacctaaca aacggcgcac cgcatcgtaa acgcccagcg acagttgttc gatgagctgc
     5281 ccgctctgct tcacactggc gttatctgcc gcaagtcgct ggacaatgcc cgcttgagta
     5341 cgaatagcag tgatggtctg accgatatca tcgtgcagct cacgcgccac gtcgcggcgc
     5401 acgctctctt cagtttctaa caaacgttca gccagatgct gattgcgcgc cagttccttt
     5461 tgcagcgact ggttaagttc acgcaaccgc tggatgcctg cgcccagtaa caaccctgtc
     5521 agactttgca ccagcagcga gagcaataaa tccaccgaat gatcgcgcca ggtttgactg
     5581 gcgatcagcg cgatggcgtt catcaacgtc gcaatcagcg ccccttgcca gccatagtgc
     5641 caggccagcg cgataatcgg cagcgccagg cagaatggcg taaagcgcga cagttcgtcc
     5701 ggcaatccca actggagcca gagactaata acaaacagca gcaagtacca gaccagatgc
     5761 cgcccgcgcc agttgatcgg ttgagaaact aacgacggcc cgagcggcag ccagatgtta
     5821 ttggcgagat agtgccagaa caccagacat atcggggcca gcgtcaagcc gccagttaaa
     5881 gtcagcaaca gcgcattcca cgactctttg ccgtgccaaa gccagggcag cgactgcaac
     5941 aacgccgccg ccgtcagcgc cgcaccctgt aacagcaagg tgcgccagtc acgttgatgg
     6001 cgatagcgcg agatcagcgc cacgggcaat aacgtcagta aactaccgat catcaataag
     6061 gagaaatggg ttaaaccgac cgcctgcgtt agccagtaaa tcagcagcca ttccgcgccc
     6121 agcaatacgg gccagtatcc gcgcgggcat tgcagcatta gccccagacg cagaccaaac
     6181 ggaaataaca gcaccgccat atcagggcgc tcaaccaggt gcaggctgat actccacagg
     6241 caaaaccatg cggcagagaa gataaaaaag caggcaataa cggtaattaa gcgggagaac
     6301 aacgtcttca tcaccagcca tcaaacatgc ggcgcgccag ttcaacgtcg ttactgacgc
     6361 ccagtttttc catcagattg gcgcgatgga cgtgtaccgt tttcggtgac aagcccagtt
     6421 cggcggcaat ctctttcacc gccattcctt gcgccagttt ttccgccacc tggcgttcac
     6481 gtttggttag cgggtcctgg cgaccggatg ccagtttaat ggcaatatcc ggcgtcagat
     6541 aacagccgcc cgtggcaacc gtatgcaccg cagcaatcag ttcatccggg ctacagcgtt
     6601 tggagagaaa gccgcgcgcc cccgcgttaa gcgcctgctc aaccagcgcc ggactgtcat
     6661 gaacagagag cataatcgtc gccatacctt tcggcagctg gcttagcagc tccagaccgg
     6721 agatatcagg catggagata tcgcaaatac acacctgcac accgcgcccc ggcaaccccg
     6781 ccagcgcctc gcgccccgaa ctaaactcgg caactacttg caaatcaggt tccagcccca
     6841 gcaactgcgc aaagccggag cggacgatga ggtgatcgtc tataagggca acggtgatca
     6901 taattgggtc ctggcggata aaaaacgcgc ataccttaac gataagcgcg atgttgttca
     6961 agttggatat ggggtatgta ttggcttaat gatgttgggc ttatcgtcaa aacgcttacc
     7021 tgaaaggtaa gcgtttggta cgtattattg agaaaaagcg aattatttgt aggttgcgat
     7081 tgcaatattg cctgaaaaat ttctcctcct gttgcggaaa tcgttttcag gtaacaaaga
     7141 gtcatccaga aacctataat cttcagtgga acgctcatta agagagtcat ccactgagtc
     7201 gttatgaata taatcctcac cgtatgttcg ataaatgaaa ttagcaatat taaaatcgtt
     7261 accagcttca tttatttcgg cttttacaat ataatgagtt tgtgtaaatg tatgcagtga
     7321 aaagtcagca aaaagaaaat ttgtttttcc catcaaagca taaaaccctg ggttactatt
     7381 aaacccggca gaaatagaat tacttgcctg tattgcatta atatcccccc tatcaagcgc
     7441 gacaggaaat gcctgtgaac aagcataata caggttacct attcctgaaa tactggagag
     7501 gcaagaaata atattttctc tggttttttt ctcaacacca tctttctgta gagagtttct
     7561 cacaatatta cattcatctg gcgtaaatac aatttcacta aaaagttttt cgaaatcttt
     7621 atattttcta acacctccat gaccagacaa agccatggtt ttactcctca gtaattcatg
     7681 gatagtgcta tttattttaa ctccacaaca tgcaataaat gagctagcct ctccaacatg
     7741 tcttgatgca tcagcaagaa gtgcactaaa ctctccctcc cgaacatcta gtttttttat
     7801 gtctgattta taactacaat catctggaat taatttatct ggaatcaatt catattggat
     7861 tgatttatct ggagttgatt gagtttgttc atataacgct ttttttaatt ggt
//
LOCUS       DACBVI010000185         7841 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.186, whole genome shotgun sequence.
ACCESSION   DACBVI010000185 DACBVI010000000
VERSION     DACBVI010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.186"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(150..302)
                     /locus_tag="IU325_20190"
     CDS             complement(150..302)
                     /locus_tag="IU325_20190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HAP5811321.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(567..1301)
                     /gene="cysH"
                     /locus_tag="IU325_20195"
     CDS             complement(567..1301)
                     /gene="cysH"
                     /locus_tag="IU325_20195"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HAP5811322.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRTNLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(1375..3087)
                     /gene="cysI"
                     /locus_tag="IU325_20200"
     CDS             complement(1375..3087)
                     /gene="cysI"
                     /locus_tag="IU325_20200"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HAP5811323.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3087..4886)
                     /gene="cysJ"
                     /locus_tag="IU325_20205"
     CDS             complement(3087..4886)
                     /gene="cysJ"
                     /locus_tag="IU325_20205"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HAP5811324.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAAIEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVNVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5202..5567
                     /gene="queD"
                     /locus_tag="IU325_20210"
     CDS             5202..5567
                     /gene="queD"
                     /locus_tag="IU325_20210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HAP5811325.1"
                     /translation="MMSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGE
                     VDPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPL
                     LSAVMVKETCTAGCIYRGE"
     gene            5645..6916
                     /locus_tag="IU325_20215"
     CDS             5645..6916
                     /locus_tag="IU325_20215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP5811326.1"
                     /translation="MEDNCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLLGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6907..7167
                     /locus_tag="IU325_20220"
     CDS             6907..7167
                     /locus_tag="IU325_20220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAP5811327.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQTVQRLINACPAGLFSLTPE
                     GDLRVDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7184..7759
                     /locus_tag="IU325_20225"
     CDS             7184..7759
                     /locus_tag="IU325_20225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HAP5811328.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
BASE COUNT         1743 a         2095 c         2051 g         1952 t
ORIGIN      
        1 cattatgaat aatcaaataa ttttcatccg taatatatat gaataataaa cataggcata
       61 atattcacat aaacaatata aattaaaaaa gggtgcagga gaccaaacca tcacaattca
      121 atacgcgggg ttttaccccc gcgtggtggc tacttcttcg attcgtaagc gagaactgcc
      181 ttaaactcca taccacgttc tctgatactc aactcacaca acgagtcgcc taccatcagc
      241 gtgaatccca gtactgtaca acacagtacg atgattgcca caagggcata ttttgtcagc
      301 atgtcttgcc tccctgcaaa gaagagacta acattcgaat tgcttaggtt cgaacgctgg
      361 cctcaggttg atagaaatat cgcctggggc ttttgtccat ctggaacctc gcgaatgctt
      421 aacgccagac agcctcaagc acccaacgcc attctatacc tgataattct tccagcggcg
      481 ttcacgttta aaatcaggaa tgctgctcgc aaaataaaaa cgcccccatc atttctgaca
      541 gaggcgttta atttatccgg caatatttac ccttcgtgta acccacattc tctttttaag
      601 ccaaagaaac gcgtttcttc ttccgccatg ccgggttccc atttacgggt tgtatgggta
      661 tcaccgaccg ataaatatcc ttcatcccat aatgggtgat atttcaggcc atgtttttgc
      721 aggtactgat aaatagttcg gttatcccag tcgataatcg gcagcacttt aaatacgcca
      781 cgctgaattg ccagcaccgg taaattggtg cggctgccgg attgttcacg gcgcaggcca
      841 gcaaaccagg tttgcgcatt cagttctttc agcgcccggt tcatcggttc gactttgttg
      901 atgtcattgt acttttcaat gccttcaaca ccctgctccc acagttttcc gtaacgtgct
      961 tcctgccagg cggcgctttc ggtagcgcgg tacactttca ggttgagctt gagtttgtcc
     1021 gttaactcgt caataaagcg gtaggtttcc gggaacaagt aacccgtatc ggtgaggatc
     1081 actggaatat ccgggcgaat ctgattcacc agatgcaggc tcaccgccgc ctgaatgccg
     1141 aaactggaag aaagtacata ttcaccgggc agattatcca gcgcccaggc aacgcggcct
     1201 tcagcgtcca gtttttccag ttcggcgtta gtttctgcca gcgccagaat gcgatctacc
     1261 ttcggcagtt cgttcagggc gtttagatcg agtttggaca tagattcctc actgtttgcc
     1321 ttgcctgatg cgacgctcac gcgtcttatc aggcctacaa gaccgggctg atggttaatc
     1381 ccacaaatcg cgcgccggat cgagcaccgg gcgaatgatg cccgcacgca ccgtaaagtc
     1441 gccgaagcct tcacccgctt cgcgctcttt cgcccagcgc cctatcagtt catcaagcga
     1501 cgccaggatt tccggttcgg tgatgttttc tttatacatc cgtgggatac gtgtcccaat
     1561 gcggttgccg ccaagatgca ggttgtagcg acccggcgct ttacccacca ggcccacttc
     1621 cgccagcatc gcgcgaccac aaccgttcgg gcagcctgtt acacgcatca cgatatgctc
     1681 atcgctgaca ccatgtttcg ccattaaatt atcgatgttg tcgataaaag acggcaggaa
     1741 acgctcggct tcggccatcg ccagcgggca agtcgggaat gacacgcagg ccattgagtt
     1801 ttcacgctgc ggcgtgacgg cattcattaa cccgctctct ttggcaatct tctcgatctt
     1861 cgctttctcg ctttccggca caccggcgat gatcagattc tggttcgccg taatgcggaa
     1921 atcgcctttg tggatcttcg cgatctccag caggccggtt ttcagcggac gcccaggata
     1981 atcaaggatg cgaccatttt cgataaacag cgtcaggtgc cagttatcat caatgccttt
     2041 aacccagcca atacgatcgc cgcgtccggt gaactcatat ggacggatcg gttcaaattt
     2101 gatccccgcg cgacgctcca cttccgcttt aaacgtctca acccccacgc gctccagcgt
     2161 gtatttggtt ttggcatttt tacgatcggt acggttaccc cagtcgcgct gagtcgtcac
     2221 gacggcttct gccaccgcca gcgtatgctc cagcggcaga tagccaaact cactcgccgt
     2281 gcgggcgtag gttttcttgt tgccgtgttc gatagaaagc ccaccgccca ccaacaggtt
     2341 aaagcccacc agcttgccgt tttcggcgat tgccacgaag ttcatgtcgt tggcatgcag
     2401 atcgatatcg ttctgcggcg ggatcactac cgtagttttg aatttacgcg gcaaataagt
     2461 ctgaccgagg atcggttctt catcggtggt ggcgactttt tcctgatcga gccagatctc
     2521 cgcatacgcg cgggtacgcg gcagcagatg ttcagagatt ttcttcgccc attcatacgc
     2581 ttccgcgtgt agctgcgact cgtaagggtt cgaggtgcag agtacgttac ggttcatgtc
     2641 gttagcagtc gccagcgcat caagaccgac cgagtgcagc atctggtgca ccggtttgac
     2701 gttctttttc agaatgccgt ggaactgaaa cgtctggcgg ttggtcaggc gaatgctgcc
     2761 atagatggtg ttttcaccgg caaatttatc gatcgcctgc cactgtttgg tggtaatcac
     2821 gccacccggc agacggcagc gcaacagcat tgcatgacgt ggctccagct tctgttcagc
     2881 acgttcggcg cggatgtcgc ggtcatcctg ctgatacatg ccgtggaagc ggatcagcag
     2941 gaagttatcg cccttaaagc cgccggtcag accgtcgttt aaatcttccg caatggtgcc
     3001 gcgcaggtag ttgctttcaa gcttcatgcg ctcggcgtct gtcagttttc cttcgaccac
     3061 taaaggccct ggatgttttt cgctcattag tagacatctc gctgataacg gcgctctacg
     3121 cgcagctcac ttaaaaattc atccgccgct tcggtgtcca tgccaccaaa ttcggcaatc
     3181 acttccagaa gtgcctgctc aacgtctttc gccatgcgat tagcgtcgcc gcagacataa
     3241 atgtgggcac catcattgat ccagcgccac agctctgcgc cctgttcgcg cagtttgtct
     3301 tgtacgtaaa ttttttcttt ttgatcgcgc gaccaggcaa gatcgatacg cgtcagcacg
     3361 ccctctttga cgtagcgctg ccattccacc tggtagagga aatcttccgt aaagtgcggg
     3421 ttgccaaaga acagccagtt tttccccggc gcttcgtcgg cggcgcgttg ctgcataaag
     3481 gcgcggaacg gcgcgatgcc ggtgcctggg ccaatcataa tcaccggcgt ttgcgggtta
     3541 gcgggcaggc ggaagttatc gttatgttcg ataaagacgc ggacttcacc ctcttcttcc
     3601 acgcgatccg cgaggaagct ggaggcacca ccggcacggg cgcggccttc cacgtcgtaa
     3661 cgcaccacgc caacggtgac gtgtacttcg ttctcgactt ccgcctgcga ggaggcgatg
     3721 gaatacaggc gcggggtcag cgggcgcagc aggttaatca gcgcttcggc atccagttgc
     3781 gccggagaga aacgcaccat gtcgacaatc ggcgtcgtcg cggcgtaatg ctgtaacttc
     3841 gctttatcgc ccaccagcgg cagcagcgtt tcgctgcggg taagcgtggc atagttctca
     3901 acaatgttgg cggtgttgac ggtcagttcg aagtgccact gtagcgcttc gttcagcggc
     3961 agggttttgc cctcaacgtt gacaggttca tcacctttca gccacagcag ttcgacaagt
     4021 tctttcacca gtgccggatc gttctgatac cagacgccca gcgcgtcacc cggctggtaa
     4081 cgcaggcccg agtcacctaa gtcaatttcg atatggcgaa cgtctttttc agagttacgc
     4141 ccggtaattt tctggttaac agagaggctc gccaccagcg gcgcgtcttt gctgtacggg
     4201 ctggtgtgga tttcatttac cgcgccagta gcgacggatt gcgaaggtgc cgcgacaggc
     4261 gcacgcgatt taagcgcatc aaccacgcgg gcgcgccact cgatggcagc agcctggtat
     4321 tcaacgtcgg catcgacacg gtcgagcagg cgttcaccgc ccagttccgc cagcttgctg
     4381 tcaaaatctt tcccggactg gcagaaaaat tcataagagc tatcgccgag gctaaacacg
     4441 gcaaatgcgg tgttttccag cttcggcgct tttttggaga acaggaactt atgcagcgcg
     4501 acggcttctt ccggcggttc cccttctcct tgcgttgacg tcaccacgat aagcagtttt
     4561 tcgctggcga tttgtttgaa tttatagtcg cccgcgttca ccagcttaac gttcagtttt
     4621 gccgccaata aatcatcgcg taatgcttca gcaacccggc gcgcattgcc ggtttgcgaa
     4681 gcggagataa tagttatacc cggcatttct gcggcgggcg ctggcgtcgc tgcaagcgca
     4741 gctggctgct ggttgagcac gccccagaaa tagccagaaa cccaggcaag ctgggtggga
     4801 gttaaatcgg tcgtggccgc ctgaaggcgt gccagttgct ccgggttcaa cggaagcaac
     4861 gcggaaggtg ggacctgtgt cgtcatgcgt cgttatgttc cagtaagcaa agctgtttct
     4921 gcgccctgtc agcgcccata aaacagaaga gaagataagg ttaacggggt gaacggtgtg
     4981 gattaaagac gggatagcga taactaataa ccaaatcgac taacctgttt tagcaatagt
     5041 ctttaacaac aaaatagatt aaccaaccca atgaaaaaca aatgaatatt gcccatcaat
     5101 aagataaatc agcgattttg cgcaacaaag tcgttttaga taatgcgaaa aaacagcctt
     5161 tccggtactc tacggcggtt ttatcgtcct gtagagaaat tatgatgtcc accacgttat
     5221 ttaaagattt caccttcgaa gccgctcacc gcttaccaca cgtcccggaa gggcataaat
     5281 gtggtcgcct gcacgggcat tcctttatgg tgcgactgga aattaccggg gaagtcgatc
     5341 cgcatacggg ctggattatc gatttcgctg aactgaaagc ggcgtttaag ccaacctacg
     5401 agcgcctcga tcaccattat ctcaatgata ttccaggtct ggaaaaccca accagcgaag
     5461 ttttagcaaa atggatttgg gatcaggtta aacccgttgt gccgctgtta agtgcggtga
     5521 tggtaaaaga aacctgtact gcaggctgta tctatcgcgg cgaatgataa gagtgtgtcg
     5581 gcggtcaatt tcccttaagt aacgccatgt tagggtgttg tgttctggat atctggggca
     5641 tgacatggaa gacaactgcg acattattat tattggtgcc ggtatagcag gtaccgcttg
     5701 tgctttacgc tgcgcgcggg caggtttgtc cgttctgtta ctggaacgcg ctgaaatccc
     5761 cggcagcaaa aatctttccg gcgggcgttt atacacccat gcgctcgcgg aactcctccc
     5821 gcaatttcat ctgaccgcgc ctcttgaacg acgcatcact cacgaaagcc tttctctgct
     5881 aacgccggat ggcgcaacga cgttttccag cttacagccc ggcggtgaat cctggagtgt
     5941 attacgtgca cggttcgatc cgtggctggt tgccgaagcc gaaaaagaag gtgtcgaatg
     6001 catccccggt gcgacggtgg atgcgctgta tgaagaaaac ggcagggtgt gtggtgtcat
     6061 ttgtggtgac gatattctcc gcgcccgtta tgtggtgctg gcagaaggtg ccaacagcgt
     6121 cctggctgaa cgtcacgggt tagtgactcg tcctgctggc gaagcgatgg cgttggggat
     6181 caaagaagtg ctgtcgctgg aaacatccgc tattgaagaa cgttttcatc tggagaataa
     6241 cgaaggcgca gcgttgctgt tcagcggcgg gatctgtgat gacttacccg gcggcgcatt
     6301 tctttatact aatcaacaaa cgctctcgtt agggattgtt tgcccgctct cttcccttac
     6361 gcaaagtcgt gttccggcaa gcgagctgct ggctcgcttt aaaacgcatc cggcagtgcg
     6421 cccgcttatc aaaaacacgg aatcactgga gtatggtgcg catctggtgc cagaaggtgg
     6481 cttgcacagt atgccggtgc aatacgccgg taacggctgg ctgctgctgg gcgatgcgtt
     6541 gcgcagttgc gtcaataccg gaatttccgt gcgcggcatg gatatggcgc taactggcgc
     6601 gcaggcggcg gcacaaacgc tgataagcgc ctgccagcac cgcgagccgc aaaatctgtt
     6661 tccgctttat catcacaacg tcgagcgcag cctgctatgg gatgttctac aacgttatca
     6721 gcatgttccg gcgcttttgc aacgccctgg ctggtaccgt gcgtggcctg cgttaatgca
     6781 ggatatttcc cgcgatttat gggatcaggg tgataaacct gttccaccgc tgcgccagtt
     6841 attctggcgt catttacgtc gtcacggcct gtggcatctg gcgggcgatg ttatcaggag
     6901 tctgcgatgt ctgtagcccg taatctctgg cgcgttgccg atgcgccgca cattgttccg
     6961 gctgactccg ttgagcgcca gacggtacaa cggttgatta acgcttgtcc ggctggtctt
     7021 ttttcgctca caccggaagg tgacttacgc gttgattatc gcggctgcct ggagtgtggc
     7081 acctgccgtt tgctgtgcga cgaatcaaca ctacaacagt ggcgctatcc gccttccgga
     7141 ttcggcatca cctaccgctt tggataaata ataaggataa tttatgcccc tcttacacct
     7201 gctccgccag aatccggtga ttgctgccgt taaagataat gccagcctgc aactggcaat
     7261 cgattctgaa tgccagttta tttcagtgtt gtacggcaat atctgcacca tcagtaatat
     7321 cgttaaaaag attaaaaacg ccggaaaata tgcctttatt catgttgatt tgctggaagg
     7381 cgcatcgaac aaagaagtgg tgattcagtt tctgaaactg gtgaccgagg cggacggcat
     7441 tatcagcacg aaagcatcga tgctgaaagc ggcaagagcg gaaggttttt tctgtattca
     7501 tcgcctgttt attgttgatt cgatttcgtt tcacaacatt gataagcaag ttgcgcaatc
     7561 gaatccggat tgtattgaga tcctgccagg ctgtatgcct aaagtgctgg gctgggtgac
     7621 ggagaaaatc cgccaaccgc tgattgccgg cgggctggtg tgcgatgaag aagatgcgcg
     7681 taatgcgatt aacgcgggtg ttgtggcgct ttccaccacg aataccgggg tctggacgtt
     7741 agcgaaaaaa ttgctttgac gggatactcg taacgagttg aatttgtgtg atttgtaggc
     7801 tgcacgccac atccgacatt cagcgcctga tgcgacgctt g
//
LOCUS       DACBVI010000186         7770 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.187, whole genome shotgun sequence.
ACCESSION   DACBVI010000186 DACBVI010000000
VERSION     DACBVI010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.187"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            123..851
                     /gene="gadW"
                     /locus_tag="IU325_20230"
     CDS             123..851
                     /gene="gadW"
                     /locus_tag="IU325_20230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HAP5811329.1"
                     /translation="MTHVCSVILVRRSFDIYHEQQKISLHNESILLLDKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQIPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNDSG
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1214..4327)
                     /locus_tag="IU325_20235"
     CDS             complement(1214..4327)
                     /locus_tag="IU325_20235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HAP5811330.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLAAGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4352..5509)
                     /gene="mdtE"
                     /locus_tag="IU325_20240"
     CDS             complement(4352..5509)
                     /gene="mdtE"
                     /locus_tag="IU325_20240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HAP5811331.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGTLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5569..5847
                     /locus_tag="IU325_20245"
     CDS             5569..5847
                     /locus_tag="IU325_20245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811332.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLTSPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5848..6375)
                     /gene="gadE"
                     /locus_tag="IU325_20250"
     CDS             complement(5848..6375)
                     /gene="gadE"
                     /locus_tag="IU325_20250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HAP5811333.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(7173..7745)
                     /locus_tag="IU325_20255"
     CDS             complement(7173..7745)
                     /locus_tag="IU325_20255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HAP5811334.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGVALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT         2050 a         1863 c         1834 g         2023 t
ORIGIN      
        1 ctttgtggat aaattttggt cctaccaaat ctggcagttt ttgcgctaag aaacagtctg
       61 gcatcatttc attagtatac tgaaattgaa ataatcgcag taatgaaata taagggatag
      121 tcatgactca tgtctgctcg gtgatcctcg ttcgtcgttc attcgatatt tatcatgaac
      181 agcaaaaaat atcgttgcac aacgagagta tcctgttact ggataaaaat ttggcagacg
      241 attttgcgtt ttgttcaccg gatacgcgac gactggatat cgatgagctg acagtttgcc
      301 attacttaca aaatattcgt cagataccac gcaatttagg gttacatagc aaagaccgtc
      361 tgttaattaa ccagtcaccc cccatgccgc tggtgacggc gatttttgat agtttcaatg
      421 actccggggt aaattcaccg atactgagca aaatgctcta tctttcctgt ttatcgatgt
      481 tttctcataa gaaagaactg atccccttac ttttcaatag tatcagtact gtttcaggaa
      541 aagttgaacg ccttattagc tttgatatcg ccaaacgttg gtatctgcgc gatatcgcgg
      601 aaagaatgta taccagcgag agtctaatca aaaaaaagtt gcaggatgaa aatacctgtt
      661 tcagtaaaat attactcgcc tccaggatgt cgatggccag acgattactc gagttacgtc
      721 aaattcctct gcatactatt gcggaaaaat gtggctatag cagtacatcg tactttataa
      781 acacatttcg acaatattat ggtgtaacgc cacatcagtt tgcgcaacat tcgccaggta
      841 ccttttcctg acatattttg catttgaata ttggtcagga tctcacacct gcttcatgtg
      901 aaactcttcc ctgatgattt ctgccgggct accggctagt tctctttcgc agtttgccag
      961 gcggtatagg tattttcagg aggaaaagac tcggcatgtt tgggattatt aagctgacaa
     1021 ttcatacgat tacgggaatg tgggtcagta ttgtgttgtt taaattgatg acgaatggct
     1081 ggagtggttt ctactttcag tgctgtgtac taagcctggt attcctgacg gttagctggt
     1141 tgttgagtgg tgaatggtta gcgggaaaga gtaaggctga accctcacgt tcaaccttac
     1201 tctcatttac acgttacgct tttttaaagc gggcaaagag atgttccact acaacgaaaa
     1261 agaccggaac gaagtaaatt gccagcactg ttgcggcaaa catcccgccc atcacgccgg
     1321 tacctaccgc gttttgcgcg ccagaaccgg caccatgact gataaccagc ggcagcacgc
     1381 cgagaataaa ggccagagag gtcatcagga ttgggcgtaa acgcatccgc gccgcttcga
     1441 tgattgcctc tatcggcgtt ttcccttctt tctgcatcat ctcaacggca aattcgacaa
     1501 tcaggatggc gttcttggcg gaaagcccga tggtggtcag caaaccaacc tggaagtaga
     1561 cgtcattact taagccgcgt agatcggtgg ccagtaatgc gccaacgacg cctaacggta
     1621 caaccaacat caccgagaac ggaattgacc agctctcata aagtgcggcg agggcgagga
     1681 acaccacgac cagtgaaatg gcatacagcg cgggagcctg atttgaggat aacgcttcct
     1741 gatacgacag tccggtccat gagtagccga cgcctgccgg aagtttagcg accaggtctg
     1801 ccataaattt catggcgtca ccggtacttt tcccggccgc cgcttcacct aaaatctcca
     1861 ttgacgggat gccgttgtag cgttccagtc gcggtgaacc ataggtccat tcggtagacg
     1921 aataggccga aagcggtgcc atcgtgccag aggcgttgcg tacataccat tggttgatgt
     1981 tatccggcaa catacggaac ggcgtgcctg cctggacata cacttttttc acccgcccct
     2041 ggttgaggaa gtcgttcacg tagctgctgc cgaaggcggt ggaaattgtc tggttgatat
     2101 cagacagcgc cacacccata gcctcggctt tcgcggcgtt gacgttcact ttgaacatcg
     2161 gcgtatcttc caggccgttc gggcgtacac cggtgacttg attcggtgat tgcgctgcca
     2221 gtgataacag ctcgtttcgc gcctgggtta gtttttcgtg ccccaggtta ccgttgtcca
     2281 gcagttccat atcaaaacct gacgcggtac ccagttcagc caccgcgggt aagttgaacg
     2341 ggaagacgac ggctttattg atactgctta acgcaatcat tgcccgctga atgatcgcgg
     2401 taaccgagtt ttcctcaccg acacgttcag accacggctt gagactgata aacgccaggc
     2461 cgttgttttg cccctggccg ctgaagccaa agccgccaac ggtaaatacc gactgaacat
     2521 tatctttctc tttagtcaga taataatccg tcacctgttg cagcactttc gtggtgttaa
     2581 ccatggtggc accggaaggt aactgcgcgg tggtcataaa taccccctga tcctcttctg
     2641 gtaagaaaga ggtcggcgtg cgcaggaaca gcaccgccat cccggcgcaa atcagcaggt
     2701 agaccaccat gtagcgaccg gtacaacgca acagcgagcg ggtactgtcg gtatagtgct
     2761 gagttgattt ttcaaacagc gtgttgaagc gtgcgaacag ggcgttaggt ttgtgaccgc
     2821 cttccggcgc ggctttcaga atggtggcgc acagggcagg ggtcaggctc attgccacaa
     2881 atactgaaag cagcatggag gagatcagcg tgatggagaa ctggcggtag atctccccgg
     2941 ttgcaccgct cataaaggcc atcggcataa acactgcgga aagaacaacg gcaatcccga
     3001 ccagcgcacg ttggatctgc cccatcgatt tatgcgtcgc ttccttcggc ggtagtttat
     3061 cttccgcaat gacacgctcg acgttctcca ccaccacgat ggcgtcatcc accagtaacc
     3121 ctatcgccag caccatcccg aacatagtta acgtgttgat ggtgaaaccg accgccgaca
     3181 agatcgcaaa cgtcccgaga ataaccaccg gtacggcaat cgtcgggatg attgtggcac
     3241 ggaaattctg caaaaacaga tacatgacca ggaagacgag gatgatagcc tcaaccagtg
     3301 ttttaaaaac ttcctgaata gaaatttcga taaacggcgt ggtgtcgtaa ggataaaccg
     3361 tcttcagact tgcggggaaa taggctgata agcggttcag ctcctctttg actgcccgcg
     3421 aggtatccag ggcgtttgct ccggcagcca gtttgatggc gatcccggca gcaggtttgc
     3481 cgttatagcg cgccacggtg gaatagtctt ccgctccaag ttcgacgcgg gcgacatcac
     3541 gcagcagcac ttgcgaacca tcttgctgaa ctttcaacag gattttgcca aattcttccg
     3601 gcgtttgcag acgcgtctgc acaatgatcg aggcgtttag ttgctggtct gccgcctgtg
     3661 gcatgccacc cagttgacca ccagaaatct ggttgttctg caccttaatc tgggaaataa
     3721 catcggaagg taccagattg tatttattga gtttttgcgg gtccagccag atacgcatgg
     3781 cgtactcgga accaaagagc tgtacgctac caacgcccgc ggtacggctt agcgggtctt
     3841 tgatattaga cgctacatag tccgcgatat cgtactggtt gaggctgccg ttatcagaaa
     3901 taaacgccgc taccatcagg atattactgc tcgacttatc gacgctaatc ccctgctgct
     3961 gcaccgcttc aggtaatgaa ggcatagcga gctgcagttt attttgcacc tgaacctgtg
     4021 cgatatcagg cgatgtccca gtctcgaagg tcagagtgat agaggcattg cccgccgcat
     4081 cactggttga agacatgtac atcaggccat caagcccatt catattttgc tcaatcacct
     4141 gagtgaccga gtcttctacc gtttgcgcat cggcacctgg ataggttgcg ctgacggtta
     4201 tggtcggtgg cgcaatctgc ggatactgcg caaccggtaa gttcatgatc gccagaccgc
     4261 ctgcaagcat cataataatg gcaagtaccc aggcaaaaac cgggcgatca ataaaatagt
     4321 tagccatgaa agtcccctta agcctgcaac gttattgttt cgattcggtg ctggcgtttt
     4381 cctggctgga ggaaattgct cgtgctttga tacccggacg aatgcgttgc aaaccggaaa
     4441 cgatcacccg atcgccagcc tgcaagccag aggtgacaac ccactggtcg ccgatggctt
     4501 tgctggcttc gatttcgcgt agctgcacga catcgtcttt atccagaatg agcgccgttg
     4561 ctttaccctg ggcgttgtgg gtgacgcctt cctgcggcac cagtaataca ttctggcggc
     4621 taccttcatc cactaatgcc gtaacgtaca tgccaggcag caagtcacca tttgggttgg
     4681 ggaaaatcgc ccgtaacgtc acggagcccg tggtttcatc caccgtcggg tcggagaatt
     4741 tcagcgtgcc ggtctggctg taacgtttac cattttccag attgagctgt actggcgtac
     4801 tgccctgaac ctgtttgatt tgcccactgg cgacctcttc tttcatacgc aagaaatcct
     4861 gcaccgactg cgtgagatcg acataaatcg ggtccagacg ttgtacggta accagcgaat
     4921 ctgcctgatt agcggtaacg agtgcgccga cggtcaccga cgatttcccg ctgacgcccg
     4981 taatcggcga ggtgacattc gcgtattgca aattgatcgt cgcctgttca acagccgctt
     5041 tggcgacggt gacattggct tctgcttcat tcagctgggt gcgcgcggtg tcgtaatcct
     5101 gacggctaac gtagttggtc ttcagcaacg atgcctggcg gttaaaggtg atgcgggcat
     5161 tgctggcggt agagagcgct ttcgccagcg tgcctttggc ggagttcagc tcggcctgta
     5221 aaggtgcagg atcaatctga tacagcgaat cgccctggtt cactttatcg ccttcgataa
     5281 agttgcgttt aatgataata ccgccaacct ggggacgtat ctcggcaact tcataaggaa
     5341 cggttctacc gggcaattcg ctcaatacat tgaccgaacc gggggagagt gtgacgacac
     5401 cgacctcagg cgtcatggcg gcggcgtttt ccgccgattt gtcatcgcag gcggtgagca
     5461 tcgcgccgca gaataacaac ggtattaaca gctttcttct tctgttcatt ttagtccctg
     5521 aaaattcttg agaaataacg gttctttaaa ttgcgcgcga ggagagatat gagggattta
     5581 cagaccagcg gcatagtggg gttgtcagcc agtaaggtgg gatacaggca tagtgatcga
     5641 catggtgagg tcaacgacaa aaaaaatgtt gccgttctgc caacagttcc tgccagcatt
     5701 cgggcaacga agggttctac tggtggatac acataccagg ggaataaagt cgttttaacc
     5761 tctcctttag taatcaccgg gggtaatgaa atcagcatct gcatccctcg tcatgccagc
     5821 catcaatttc agttgcttat gtcctgacta aaaataagat gtgataccca gggtgacgat
     5881 gtcgctcata cgtcttagtt tcagcttgta cataatgttg aatttctgaa tctttaatgt
     5941 ttgttgcgaa atattgagcg tcgacgtgat attgctttca tttttatttt tcagaatcag
     6001 ttgcataatt ttgaactgat tatgtgaaaa acataattgt gaattcttat ggggcaagtg
     6061 tttaccataa gtaatctcaa gaagattttt gatactttct ttgcggctga caaaataaat
     6121 aggcgcttta gcttttagtt tactgatgtg ataaggtgcc aaaacaataa ttctctcggc
     6181 atctaatttc tccagaaatt taatcgcttc ttcatcaagg atatgattgt gataggtatc
     6241 aatgataacc ttgaagggtt tattgcctac ttttttgatc tctgacaggg aattgatttt
     6301 tatcatttcg tgattatctt tcaactgcca aaagccctgt aaaagaaaag aatctttcgt
     6361 catgagaaaa atcataactt gctccttagc cgttatcgtt tcgaacatgt catccttttc
     6421 attatttaca tccttgtccg aatcgttgtt caatatagta aacgcctata agaactttgc
     6481 ccttcgtgaa ctaatacgtc tattaacact aatcaatgct tcagatttat ccatcacaca
     6541 ctccatatat tgatcctaag taaaattcct ggttgttatc agtttgtaaa cggcaaacaa
     6601 aaacttaacg tttacggatt actgcccaag aataagttat caataaccag tttgtgatct
     6661 ctgaagaata ttactaaagt taaaatgtaa tccgatttaa atatcgagtc tccttgtttc
     6721 gacttaagct ggcaattgga ttgccagctt tcttttaacg cattgaaatt acctgagtaa
     6781 ataactcatt catgtcttca ggtttttata tagcgtctgg agacacggat atttatgcaa
     6841 tgtaatcaac gccagttgca aaaaatgaac ccggattact tatttaaccg cccatagcat
     6901 caacgcttac ggaactcttt gttcactttc ccacagcact tttaaaacat taaacctgac
     6961 tacggaaaat atcagccatg ataatgtttg aaatggctaa tttgccatgg aatgaaaaaa
     7021 attagatgaa attcagtagg ttgaaataat catcagcagg taattatttc aatgatagtg
     7081 cgcaattgat ctacaacgct gtgtagcgga gagagtatta atcggatcat agtcacatca
     7141 agtgactatg atccgggtga caacaggaat aattattgct gcttaacgaa caaactggcg
     7201 aagctgaaca ggctggcggc gctaaatatc agttcaatac ccaccagtgt ggaaaccaaa
     7261 gttacagaca ccatcggcgt tgcaccaagg aatatccatg caatgacgat atccagcaca
     7321 ccaataacga gctgtagcca gctgcctttc attgaacgct gacgatacca actcatcagg
     7381 cgaataaccc ctgcaacaca gaacaaaccg gcaataaatg ccgcaatggc aaaaatgccc
     7441 agctccggtg cgcggatgaa gaaatagccg atcaataaat aggcgactgc gacgaggaaa
     7501 ccggataata ccggccagaa attatgactg cggttgctga ataatccaac aataagcgca
     7561 acgcccgagc agattaataa tgcacccact accgtgctta aaatatcgcc agagacgaac
     7621 gggaaactga tacacagcaa cccgacgata aacagcaaca cggcaataaa ctggattgct
     7681 ctgcgatgct ttttcagcat ctccagatca aacttcaaaa ttgttgcctt atctatatat
     7741 aacatagaac caccctataa aattaagaag
//
LOCUS       DACBVI010000187         7746 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.188, whole genome shotgun sequence.
ACCESSION   DACBVI010000187 DACBVI010000000
VERSION     DACBVI010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.188"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            203..652
                     /gene="alaE"
                     /locus_tag="IU325_20260"
     CDS             203..652
                     /gene="alaE"
                     /locus_tag="IU325_20260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HAP5811335.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADIMAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(689..1033)
                     /locus_tag="IU325_20265"
     CDS             complement(689..1033)
                     /locus_tag="IU325_20265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HAP5811336.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1185..1514
                     /locus_tag="IU325_20270"
     CDS             1185..1514
                     /locus_tag="IU325_20270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HAP5811337.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            complement(1571..2905)
                     /locus_tag="IU325_20275"
     CDS             complement(1571..2905)
                     /locus_tag="IU325_20275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP5811338.1"
                     /translation="MPRYQDIARQLKTAIEQGELKPGARLPSSRTWSQELGVSRSTVE
                     NAYAELVAQGWLIRRGQAGTFVSERIYPQQSTVQVVAFAGESQQPLPFQMGLPALDLF
                     PRELWARVMGRRLRTQTRFDLALGDVCGEVALREAIVDYLRVSRGIDCQPEQVFITHG
                     YAASIALILHALAKPGNGMWIEDPGFPLIRPIVTRHDVEILPVPVDDNGLDITSGIQN
                     YPDARFALITPAHQSPLGVALSLARRHQILEWADRSQAWIIEDDYDSEFRYHGKPLPA
                     LKSLDAPQRVIYAGTFSKALFPALRCAWLVLPVKQIAQFRHQASLAPCAVPVLWQNTL
                     ADFLREGHFWRHLKKMRQHYAQRRQWIEQALTQQGFQVVPQKGGIQMVIRMIGDDIAH
                     ARKANAAGLAVQALSDWRIRSSGEGGLLLSFTNIVNEGMARQVAQQLRKALS"
     gene            2993..3424
                     /locus_tag="IU325_20280"
     CDS             2993..3424
                     /locus_tag="IU325_20280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP5811339.1"
                     /translation="MTTLRQPYYELSPAVYNALVQAKTALENSTLDTTLMELVYLRVS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTEIART
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            3633..3878
                     /gene="nrdH"
                     /locus_tag="IU325_20285"
     CDS             3633..3878
                     /gene="nrdH"
                     /locus_tag="IU325_20285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP5811340.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3875..4285
                     /gene="nrdI"
                     /locus_tag="IU325_20290"
     CDS             3875..4285
                     /gene="nrdI"
                     /locus_tag="IU325_20290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP5811341.1"
                     /translation="MSQLVYFSSSSENTQRFIERLRLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIAQKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRHPQNA"
     gene            4258..6402
                     /gene="nrdE"
                     /locus_tag="IU325_20295"
     CDS             4258..6402
                     /gene="nrdE"
                     /locus_tag="IU325_20295"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP5811342.1"
                     /translation="MATTPAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     ITTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHASGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFTRSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6412..7371
                     /gene="nrdF"
                     /locus_tag="IU325_20300"
     CDS             6412..7371
                     /gene="nrdF"
                     /locus_tag="IU325_20300"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP5811343.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLLPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISQAQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
BASE COUNT         1957 a         1961 c         1893 g         1935 t
ORIGIN      
        1 atcggcatgt aaaaatccac aatgtacaaa aaacgcgctg ttacggaata ttttatctac
       61 aaaaacagat taaataaaaa tttttcacta attgattagt catagccagc gatatacgct
      121 atgcgaaaat gcagatgaca atgagatcca ctgctttcat ctccattaac atcccattac
      181 gcttttatta aggagcatta gcatgttctc accgcagtca cgcttgcgtc atgcagttgc
      241 agatacgttc gcgatggttg tttactgttc tgtcgtgaac atgtgtattg aagttttcct
      301 ctccggaatg agcttcgaac aatcttttta ttccagattg gtagcgattc cggtgaacat
      361 cttaattgca tggccatacg gtatgtaccg cgatctgttt atgcgcgcag cacgcaaagt
      421 tagcccgtcg ggctggataa aaaatctggc ggatatcatg gcttatgtga cgttccagtc
      481 accggtgtat gtggcgatct tgttagtggt gggcgcagac tggcatcaga ttatggcggc
      541 ggtcagttca aacatcgttg tttcgatgtt gatgggggcg gtttatggct acttcctcga
      601 ttattgccgc cgactgttta aagtcagccg ttaccagcag gtaaaagcct gacccttctt
      661 ttcgcgactg gcatcgccag tcgcgtcttt aactggcttc gccaaataaa ccattcaaat
      721 aacgttcaag cgcaacacgc gaactaaagc catgcgggat accgtaatgc tcatgacgtt
      781 cacccgctaa atagagcgga aattgctggt aatgatcgca agttttaata tcggaagcgg
      841 gttcagccag tgttgaacta cgttctttta atgttggatg aatcaccagg gcggtacgtc
      901 ccattcgcgc ttcgcgatta acatagacat aattttcccc acggcggtaa ccataagctt
      961 tctgggttac cacatcgaca gtaaaaccga ctttttcaag tacgcgcgcc acctcgtctg
     1021 gtcgtaaata catatttgat cctcgttatt atcaaccgcg ggcctacctt acctgattgc
     1081 gcattcacaa cgctttcaga aaagtccata aagccgcgat gaacctcagt taagaaaatt
     1141 atggtctaca ctgaaaatta catcgaattc taatggggga tcatatgttt aaccgaccga
     1201 accgcaacga cgttgatgat ggcgtgcagg atatccagaa tgatgtcaat caattagctg
     1261 acagcctcga atctgtattg aagtcctggg gcagcgacgc caaaggggaa gccgaagccg
     1321 cacgcagtaa agcgcaggct ctactgaaag aaaccagggc gcgaatgcat ggtcgtactc
     1381 gcgtccagca agccgcgcgt gatgccgttg gctgcgcgga ttcttttgtt cgtgaaagac
     1441 cctggtgtag cgtgggtaca gcagctgcgg taggtatttt tatcggcgca ctgttaagca
     1501 tgcgcaaatc gtagtgcaat aatgataata aatacgcgtc tttgaccccg aagcctggct
     1561 tcggggtttt ttagcttaag gctttacgta attgttgtgc tacctgtcgc gccatacctt
     1621 cgttaacgat attagtaaac gagagcagta atccaccttc cccacttgaa cggatacgcc
     1681 agtcgctaag tgcctgcacc gcaaggcctg cagcattggc tttacgcgca tgggcaatat
     1741 catcgcctat cattctgatc accatctgga taccaccttt ctgcggcaca acctgaaatc
     1801 cttgctgcgt caacgcttgc tcaatccatt gccgacgttg ggcataatgc tgacgcattt
     1861 tcttcagatg ccgccagaaa tgcccctcgc gaagaaagtc tgccagtgtg ttctgccata
     1921 gaacaggtac cgcacatgga gccagtgacg cctggtggcg gaattgtgca atttgcttca
     1981 ctggcaacac cagccacgca cagcgcaatg caggaaatag cgctttgctg aatgttccgg
     2041 cataaattac ccgctgcggt gcgtcgagac tttttaacgc cggtaacggc ttaccgtgat
     2101 agcgaaactc actgtcataa tcatcttcaa taatccatgc ctgactacga tctgcccatt
     2161 ccagtatctg atgcctgcgc gctaaagaga gcgccacacc cagcggactt tggtgtgctg
     2221 gtgttatcag ggcaaaacgc gcatcaggat aattttgtat tccgcttgtg atatccagtc
     2281 cattgtcatc aaccggcaca ggcaaaattt ccacatcatg gcgagtgaca atcgggcgaa
     2341 tcagtggaaa gccgggatct tctatccaca tcccgtttcc cggtttcgcc agcgcgtgca
     2401 gaattaaagc tattgaggcc gcataaccgt gagtgataaa gacctgctct ggctgacaat
     2461 caatcccacg tgaaacgcgt aagtaatcaa ctattgcctc gcgcaacgcc acctcaccgc
     2521 agacatcgcc taacgccaaa tcaaagcgcg tctgggtacg aaggcgacgc cccatcaccc
     2581 gcgcccacag ctctcgcgga aagagatcga gtgcgggtaa tcccatttga aatggcaacg
     2641 gttgctgact ttcaccggca aaagctacaa cttgtacagt agattgttgc ggatatatcc
     2701 gctcactgac aaatgtgcca gcctgtcccc gcctgatcag ccatccttgc gccaccagct
     2761 cagcatacgc attttcgaca gtggatcgag acacccccag ctcctgcgac caggtacgac
     2821 ttgaaggcag ccttgcgccg ggtttcagtt ccccttgctc aatagccgtt tttaactgac
     2881 gggcgatatc ctgatagcgc ggcatatggt ctgctttttt aggtaattta tggctctctt
     2941 tagcagtcca ttatagtgct acattgcggg cgttaataag aggagaaact gtatgactac
     3001 attacgtcag ccttactacg aacttagccc ggcagtgtat aacgcactag tgcaggccaa
     3061 aacggcactg gaaaatagca cgctggatac cacgttgatg gagttagttt atttgcgcgt
     3121 ctcgcaaatc aacggctgcg cattttgtct ggagatgcac agcaaagcat tgcgcaaatc
     3181 cggcgtgcca cagcacaaac tggacgccct ggcaggttgg cgcgtaagcc atcattttga
     3241 tgaacgcgag cgggcggcgc tggcatgggc ggaatcggta accgaaattg ccagaaccca
     3301 tgcggaagac gaggtttatc agcctttgct tgagcatttc agcgcagcag agatcagcga
     3361 cttaacgttt gccatcgggc tgatgaattg ttttaaccgt ctggccgttt ccatgcggat
     3421 gtaacttctt gatgccgggt attcgcccgg caaaaaaaca caaaatcccc taccccgttg
     3481 agccattcat cggtggcgta atcaatctaa aattcccata tcttgtatgg ttgaatcttt
     3541 tttcaactac atctagtatc tctgtatcaa cagagagaca acccgacgcg tatcatcgcg
     3601 ccgtatcttc attttaaacg gaaatacgaa taatgcgcat tactatttac actcgtaacg
     3661 attgcgttca gtgccacgcc accaaacggg cgatggaaaa ccggggcttt gactttgaaa
     3721 tgattaatgt cgatcgcgtt cctgaagcgg cagaagcgct gcgtgctcag ggctttcgtc
     3781 agttgccggt tgtgattgca ggcgatctta gctggtctgg tttccgcccg gacatgatca
     3841 atcgtctgca tccagcgcca cacgctgcca gtgcatgagc cagctcgtct acttttccag
     3901 cagctccgaa aatacgcagc gttttatcga acgtttaagg ctgcccgcgg tgcgcatccc
     3961 gctcaatgag cgggaacgga ttcaggtaga cgagccttac atcctgatcg tgccctctta
     4021 cggcggcggc ggtacggctg gcgcggtgcc acgacaggtc attcgctttt taaacgacga
     4081 gcataaccgg gcgttgctgc gcggcgttat tgcttcgggt aatcgcaact ttggtgaggc
     4141 gtatggccgc gccggagatg tgattgctca gaaatgcggc gtgccgtggc tgtaccgttt
     4201 tgaactcatg ggtacgcaaa gcgatatcga aaacgttcgt aaaggagtaa ccgaattttg
     4261 gcaacgacac ccgcagaacg cctgacgcag gaaacgatgg attaccacgc gctgaatgcg
     4321 atgcttaacc tttacgatag cgcaggtcgc attcagttcg ataaagatcg ccaggcagta
     4381 gacgccttta taacgacgca tgtgcgtccg aacagtgtgg ccttcagtag ccagcagcag
     4441 cgcctgaact ggctggttaa cgaaggttac tatgatgaaa gcgtccttaa tcgctactct
     4501 cgcgattttg tcattacgct gtttgcccac gcgcacgcca gcggttttcg tttccagaca
     4561 ttcctcggag catggaagtt ttacaccagc tatacgttga agacattcga cggtaagcgt
     4621 tatctggaag attttgccga tcgagtcacg atggtggcgc tgacgctggc acaaggcgat
     4681 gaaacactgg cgacgcaact gaccgatgag atgctgtcag gacgctttca gccagccacg
     4741 ccgacattcc tcaactgcgg taagcagcag cgcggcgaac tggtttcctg ttttctgctg
     4801 cgtattgaag acaatatgga gtcgattggc cgggcggtaa attccgcact gcaactgtcg
     4861 aaacgcggcg gcggcgtagc atttttgctg tcgaatctgc gagaagcggg cgcgccaatt
     4921 aaacgtattg aaaatcaatc atctggcgtg attccggtga tgaaaatgct ggaagacgca
     4981 ttttcctatg ccaaccagct cggcgcgcgt cagggggcag gtgcagtcta tttacatgct
     5041 catcatcccg atattctgcg ttttctcgat acaaaacggg aaaatgccga cgaaaaaatc
     5101 cgcattaaaa cactgtcgct gggcgtggtg atcccggata tcactttcca tctggcaaaa
     5161 gagaatgcgc agatggcgct gttttcgcct tatgacgtag agcgagttta tggcaagccg
     5221 tttgccgatg tcgccattag cgaacactat gacgaactgg ttgccgatga acgcattcgc
     5281 aaaaaatacc tcaacgcccg tgatttcttc cagcgactgg cagaaatcca gtttgagtcc
     5341 ggctatccct acatcatgta tgaagacacg gttaaccgtg ctaaccctat cgccgggcgc
     5401 ataaatatga gcaatctctg ctcagaaatt ttgcaggtta acagcgcctc agagtatgac
     5461 gagaatctcg actatgcccg cacaggccat gatatttcct gcaatttagg ttcgttgaat
     5521 attgcccaca ccatggattc ccccgacttt gcccgcacgg tagagactgc cgtgcgcggt
     5581 ttaacggcgg tgtcagatat gagtcatatc cgcagcgtgc cgtccatcga agccggaaat
     5641 gccgcctcac acgccatcgg actggggcag atgaatttac acggctatct ggcgcgagaa
     5701 ggcatcgctt atgggtcgcc ggaagcactg gatttcacca atctctattt ctataccatc
     5761 acctggcacg cactgcgtac ctcgatgttg ctggcgcgcg aacgcggtga aaccttcgcc
     5821 gggttcaaac agtcacgcta tgccagtggt gaatatttta gccaatatct gcaagggaac
     5881 tggcagccga aaacggcgaa agttggcgaa ctgtttaccc gtagcggtat tacgttacct
     5941 acccgtgaga tgtgggcgca gctgcgcgac gacgtgatgc gctacggcat ttacaaccag
     6001 aatcttcagg cggtgccgcc aaccggttct atctcttata tcaaccatgc tacgtcgagt
     6061 attcatccga ttgtggcgaa agtagagata cgcaaagagg gcaaaacagg acgcgtttac
     6121 taccctgccc cgtttatgac taacgagaat ctggcgctgt atcaggacgc ttacgaaatt
     6181 ggcgcagaaa agatcatcga cacttacgcg gaagcgactc gccatgtcga tcaggggctg
     6241 tcgctgacgc tgtttttccc cgataccgcc accactcgcg atatcaacaa agcgcagatt
     6301 tacgcctggc gcaaaggtat caaaacgctc tattacatcc gcctgcgtca gatggcgctg
     6361 gaaggcactg aaattgaagg ctgcgtctcg tgtgcacttt aaggaatatc tatgaaactc
     6421 tctcgtatca gcgccatcaa ctggaacaag atatctgacg ataaagatct ggaggtgtgg
     6481 aatcgcctga ccagcaattt ctggctacca gaaaaggtgc cgctgtcgaa cgatattcct
     6541 gcatggcaga cattaagtgt cgtagaacaa caactgacga tgcgcgtttt tactggcctg
     6601 acgctgctcg acacgctgca aaatgttatc ggcgcgcctt cgctgttgcc cgatgcactc
     6661 acgcctcatg aagaagcggt attatcgaat atcagcttta tggaagcggt tcatgcccgc
     6721 tcttacagtt cgattttctc gacgctgtgc cagaccaaag atgtcgatgc cgcctacgcc
     6781 tggagtgaag aaaacgcacc gttgcagcga aaagctcaga ttattcagca acattatcgc
     6841 ggggatgatc cgctgaaaaa gaaaatcgcc agtgtgtttc ttgaatcttt tttgttctat
     6901 tccggtttct ggctgccgat gtatttttcc agccgcggaa agctaaccaa taccgcagac
     6961 ctgatccgtc tgattatccg cgatgaagca gtccacggtt attacatcgg ctataaatat
     7021 cagaaaaaca tggaaaagat ctcccaggca caacgtgaag agttgaagag tttcgccttc
     7081 gatttgttgc tggaacttta tgacaacgag ttgcaataca ccgatgagct gtacgccgaa
     7141 accccgtggg ctgacgatgt gaaagcgttt ctctgttaca acgccaataa ggcgttgatg
     7201 aatctgggct acgaaccgtt atttcccgca gaaatggcgg aagtgaatcc ggcaatcctc
     7261 gccgcgcttt cgccgaatgc cgatgaaaat cacgatttct tttccggttc aggctcttca
     7321 tatgtgatgg gaaaagcggt cgaaacagaa gatgaagact ggaatttctg agggtgttat
     7381 ttttcaaaaa tatcactacc cgcagcaggg aaataatttc cgccaaatag ctttttatca
     7441 cgcaaatatt ttgtggtgat ctacactgac actctgttgc attattcgcc tgaaaccaca
     7501 atattcagac gttttttcgc tatctttgac aaaaaatatc aacttttctc gatttgctct
     7561 cagcccttat atcatgggaa attccggcga tttgctcaca tcaatattca tgccacattt
     7621 gccatcaggg gttgcctcag attctcagta tgttagggta gaaaaaagtg actatttcca
     7681 ttgggtaatg tatcgacata aacaaataac aggaatcttt ctattgcatg gcaattaaat
     7741 tagaaa
//
LOCUS       DACBVI010000188         7690 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.189, whole genome shotgun sequence.
ACCESSION   DACBVI010000188 DACBVI010000000
VERSION     DACBVI010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.189"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(93..356)
                     /gene="cedA"
                     /locus_tag="IU325_20305"
     CDS             complement(93..356)
                     /gene="cedA"
                     /locus_tag="IU325_20305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HAP5811344.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYIPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            539..2800
                     /gene="katE"
                     /locus_tag="IU325_20310"
     CDS             539..2800
                     /gene="katE"
                     /locus_tag="IU325_20310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HAP5811345.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPTAEPTP
                     PGAQPTTPGSLKAPDTRNEKLNSLEDVRKGSENFALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     MWSRIPKIDKIPA"
     gene            complement(2847..3596)
                     /gene="chbG"
                     /locus_tag="IU325_20315"
     CDS             complement(2847..3596)
                     /gene="chbG"
                     /locus_tag="IU325_20315"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HAP5811346.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRADRQMVFDLP
                     VNLRTTQGFSSAFYGEEISESLFLQVLDDSSHRGERSLEVMCHPAFIDNTIRQSAYCL
                     PRLTELDVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3609..4961)
                     /locus_tag="IU325_20320"
     CDS             complement(3609..4961)
                     /locus_tag="IU325_20320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="catalyzes the fromation of N-acetyl-D-glucosamine
                     and N-acetyl-D-glucosamine-6-phosphate from
                     diacetylchitobiose-6-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP5811347.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5065..5904)
                     /gene="chbR"
                     /locus_tag="IU325_20325"
     CDS             complement(5065..5904)
                     /gene="chbR"
                     /locus_tag="IU325_20325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HAP5811348.1"
                     /translation="MQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDYY
                     EFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFEQ
                     HYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRHY
                     REEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTPM
                     QIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLTE
                     FNQ"
     gene            complement(5915..6265)
                     /gene="chbA"
                     /locus_tag="IU325_20330"
     CDS             complement(5915..6265)
                     /gene="chbA"
                     /locus_tag="IU325_20330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HAP5811349.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6316..7674)
                     /gene="chbC"
                     /locus_tag="IU325_20335"
     CDS             complement(6316..7674)
                     /gene="chbC"
                     /locus_tag="IU325_20335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HAP5811350.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVHRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
BASE COUNT         2064 a         2007 c         1723 g         1896 t
ORIGIN      
        1 ctttttgtta caggtggttt gaatgaatgt agcaatgaaa tacaaaattt caggccatgt
       61 aactcccggc aaaaccggga ggtacgtaat ctttactcag tcacttcccc ttcctggcgg
      121 atctgatttg cccaacgctg ggcggattca ggcacagtaa atgccggtga gcgcagaaat
      181 gactctccca tcagtacaaa cgcaacatat ttgccacgca gcatccagac atcacgaaac
      241 gaatccatct ttatcgcatg ttctggcggc gcgggttccg tgcgtgggat atagctaata
      301 atctggcggt tttgctgacg gagcggtttc ttcattactg gcttcactaa acgcatatta
      361 aaaatcagaa aaactgtagt ttagccgatt tagcccctgt acgtcccgct ttgcgtgtat
      421 ttcataacac cgtttccaga atagtctccg aagcgggatc tggctgatgg tctatagtta
      481 gagggttttt tgaccaaaac agcggccctt tcagtaataa gttaaggaga cgagttcaat
      541 gtcgcaacat aacgaaaaga atccacatca gcaccagtca ccactacacg attccagcga
      601 agcgaaaccg gggatggact cactggcacc tgaagacgga tctcatcgtc caacggctga
      661 accaacaccg ccaggcgcac aacctaccac ccctgggagc ctgaaagccc ctgatacgcg
      721 taacgaaaaa cttaattctc tggaagacgt acgcaaaggc agtgaaaatt ttgcgctgac
      781 cactaatcag ggcgtgcgca tcgccgatga tcaaaactca ctgcgtgccg gtagccgtgg
      841 tccaacgctg ctggaagatt ttattctgcg cgagaaaatc acccactttg accatgagcg
      901 catcccggaa cgtattgttc atgcacgcgg atcagccgct cacggttatt tccagccata
      961 taaaagctta agcgatatca ccaaagcgga tttcctctca gatccgaaca aaatcacccc
     1021 agtatttgtt aggttctcta ccgttcaggg tggtgctggc tctgccgata ccgtgcgtga
     1081 tatccgtggc tttgccacca agttctatac cgaagagggt atttttgacc tcgttggcaa
     1141 taacacgcca atcttcttta tccaggatgc gcataaattc cccgattttg ttcatgcggt
     1201 aaaaccagaa ccgcactggg caattccaca agggcaaagc gcccacgata ctttctggga
     1261 ttatgtttct ctgcaacctg aaactctgca caacgtgatg tgggcgatgt cggatcgcgg
     1321 tatcccgcgc agttaccgca ccatggaagg cttcggtatt cacaccttcc gcctgattaa
     1381 tgccgaaggg aaggcaacgt tcgtacgttt ccactggaaa ccactggcag gtaaagcctc
     1441 actcgtttgg gatgaagcac aaaaactaac cggacgtgac ccggacttcc accgccgcga
     1501 gttgtgggaa gccatcgaag caggcgattt tccggaatac gaactgggct tccagttgat
     1561 tcctgaggaa gatgaattta agttcgactt cgaccttctt gatccaacta aacttatccc
     1621 ggaagaactg gtgcccgttc agcgtgtcgg caaaatggtg ctcaatcgca atccggataa
     1681 cttctttgcc gaaaacgaac aagcagcatt ccatccaggt catattgttc ctggtctgga
     1741 cttcaccaac gatcccctgt tgcagggacg tttgttctcc tataccgata cacaaatcag
     1801 tcgtcttggt gggccgaatt tccatgagat tccgattaac cgcccgacct gcccttacca
     1861 taatttccag cgtgacggca tgcatcgtat ggggatcgac actaacccgg cgaattatga
     1921 accgaactcg atcaacgata actggccgcg cgaaacaccg ccggggccga aacgcggcgg
     1981 ttttgaatca taccaggagc gcgtggaagg caataaagtt cgcgagcgca gcccatcgtt
     2041 tggcgaatat tattcccatc cgcgtctgtt ctggctaagt cagacgccat tcgagcagcg
     2101 ccatattgtc gatggtttca gttttgagtt aagcaaagta gttcgtccgt atattcgtga
     2161 gcgcgttgtt gaccagctgg cgcatattga tctcactctg gcccaggcgg tggcgaaaaa
     2221 tctcggtatc gagctgacgg acgaccagct gaatatcacc ccgcctccgg acgtcaacgg
     2281 tctgaaaaag gatccatcct taagtttgta cgccattcct gacggtgatg tgaaaggtcg
     2341 cgtggtagcg attttactta atgatgaagt gagatcggca gaccttctgg ccattctcaa
     2401 ggcgctgaag gccaaaggcg ttcatgccaa actgctctac tcccgaatgg gtgaagtgac
     2461 tgcggatgac ggtacggtgt tgcctatagc cgctaccttt gccggtgcac cttcgctgac
     2521 ggtcgatgcg gttattgtcc cttgcggcaa tatcgcggat atcgctgaca acggcgatgc
     2581 caactactac ctgatggaag cctacaaaca ccttaaaccg attgcgctgg cgggagacgc
     2641 gcgcaagttt aaagcaacaa tcaaggtcgc tgaccagggt gaagaaggga ttgtggaagc
     2701 tgacagcgcc gatggtagtt ttatggatga actgttaacg ctgatggcag cacaccgcat
     2761 gtggtcacgc attcctaaga ttgacaaaat tccggcgtaa atatggataa ggtcacgccc
     2821 cctggggagt gaccttacca ccttacttac acatcaagat aactacccaa ccgataacca
     2881 cgctcagcaa tcgcatattt caacgacgct gaagtcagca catccagttc cgttaaccga
     2941 ggtaagcaat aagcactctg gcgaatcgta ttatcgataa acgccggatg acacatcact
     3001 tccagagagc gctcgccgcg atgactggag tcatcaagca cttgcaggaa cagcgactca
     3061 ctgatctctt caccgtagaa tgcactgctg aatccctggg tagtgcgcag gttgaccggc
     3121 aaatcaaaca ccatctgacg atccgcccgc aacgcaatgc cctcttctgc cgcaaacctg
     3181 gcaacaatcg ggaaaatctg cgggaacata tgcacatgat gatggctatc aagatgcgta
     3241 ggtttgcgtc caaatagctc aatgaaacgc aaatactgac tggcaagctc ctgagtaatt
     3301 tcttccagcg gtaaagcatc ttcttctgcc aactgccaga tccattttcc cagcacacca
     3361 tcgcgggtta accccggcat agctgtcagt ggcttgccca tagtaaggac aaagtgcatc
     3421 cctatggcca gactcggttc atcacgactc aactgcaccg catggtcaat agcctgccca
     3481 ttcaccagcg ccgtcgtcga tgtgacaatc ccattgcgac aggcctcgat aatgccgtag
     3541 ttctgacctt tgcttaagcc aaaatcatcg gcattaacaa tcagtaagcg ttccataatc
     3601 agcctcggtt aatgtgcttt tttaagctct gcgatgcagt cggcaaaatt tggcagccat
     3661 ttctcgtgcg ccagaatcat ctcgcgtgcc agcagctcag catcgcgatc ggaatgcacc
     3721 aacggactaa ggtttagcgc cagtaacaca tcgttaaatt ctccgctaag tgcagcgttg
     3781 ctggcagcaa tctcgaagcc tttaatggtg tgaatcagcc ccatcacttt atcatcgaaa
     3841 tgcgtaatgc gcggatgtgg cgtcgcgcca tcgcgcccca gcgtacaggt catttctacc
     3901 gcccagtctg ccggaatatt atcaatatgc ccatgatgcg ggatattaac gtaatgttca
     3961 gcttgcttgt cgttgtagat agcgttgatc acttcgcacg ctgcatcaga gtaataagcc
     4021 ccaccgcgct gttccagttc tttcggctta actttcaact ccgggttttt atacagctca
     4081 aaaagttgtt tctcgacttt ctgtactacc tgtgctcgtg cgccgccttt gtagtattcg
     4141 cccatttcaa tagccagcat ctctttttgc ttgaagtaat aaagcaaata agaacacggc
     4201 aacagattca gagaacgaat taagccttcg ctaaatggca gatcgaaaat atttttaacg
     4261 ccagatgctt ttaactgccc ggatgccaca ccatcaagca attcggcaaa gcgtgatttg
     4321 ccatttacca gcacatcttt aatgaacacc atatggttga ggccgaacag atcgatagat
     4381 aaatcatcgc tgtctttcag catcagaaca tcgcgaataa acatcttcat gccgatcgga
     4441 atattacaca cgccgataaa gcgtttaaat ccggtatgac gataaacggc ttcagtgacc
     4501 attcccgccg ggttagtgaa gttaatcacc catgcattcg gacaaagttc ttcgacatct
     4561 tttacgatgt caaaaatcac cggaatggta cgcagacctt taaaaagacc gcccgcgccg
     4621 ttggtttcct gaccaagata accatgactt aatggaatac gttcatccag ttcgcgcgcc
     4681 ggtaattggc ctacgcgcag ttgggtagta acgaaatcag catctttcaa tgcttcgcgg
     4741 cgatccagcg ttttataaag cttcatcggg acgccagcgt tatcaatcat ccgttggcag
     4801 agatcaaaaa taatatccag tttagcttta ccaccttcga catccaccag ccataattcg
     4861 ctgaccggca attcgtgata acgcttaata aatccttcca gtaactccgg ggtatagctg
     4921 ctcccgccac caatagtgac gacttttaat ttctggctca taatttctcc cttcagtaca
     4981 gaatactgat atctggcata tctgcccccc ggacataaat aatccagcaa caggacagat
     5041 atgtgaattg tcaggtataa cgacttactg attaaattca gtcaatttct tacgatagct
     5101 cttaggcgta aaggacgtta atttcttaaa cgttttaata aacaagctcg ggctactata
     5161 accggcctca aacgcaatat ccgtcacgga atagttggtc atttccagtt gttttttggc
     5221 aaaattaata cggatctcat taataatctg cattggtgtt ttgccataat atcgttgagt
     5281 cgctcgcgtc aaatattcct gtgatttggc tgacaacgtc accatattct ccagcgccga
     5341 ttcactaaac tgctctttat catgcatctt ttctaccgta cttttcagcc actgcggtac
     5401 atcatcaatc acctgttctt cgcggtaatg acgtaaacgg ttaatgacat aaaaagtaac
     5461 catctcaaca aactcttcca gccctgtttc gcggaaattc aatgaagaaa tcactgtttc
     5521 cacataggtg agaaaagcgt tattggtccg gtatacctgc gaagcgacaa agcaataagg
     5581 caacaacggc aggtaatgct gctcaaaaaa gcgtttactg atcccaacat tcaatatgcg
     5641 cgtggcacca aactcataaa aactttgatg gtgcgaacct aacggaataa aaacaaaatc
     5701 gccccgttcc agtaacacgc gcttaccgtt aatctcctgg aaataacgcc cggttaatac
     5761 cagagtaaat tcataatagt cgtgctgatg cagtccgctg atactctcag ttttgttgta
     5821 gataaacaca tggaagtttt tgccattaaa caactgctgt tctcgggcag tggcaatttc
     5881 cggcgcgtta atcactggct gcatcatcga ctccttatgc cttcagtttt tcatgaagct
     5941 caattaattc ggtaatcagt tcacgcgcaa gcatggacgt cattaaatga tcctgagcgt
     6001 ggaccagcac cagactcact ttcatcttac cttcgcccgc atcgccttca atcagtttcg
     6061 tctgtaccag atgcgcttca ttcaatgcca tccgtgactg atccatcatg gcttttgctg
     6121 cggcaaaatc gccctgcttc gcctgtttca gtgcggcata cgccaggctg cgcgcttgtc
     6181 cggagttgat gataagtccc atcaccactt cttccagctc ttcagcttct gtttgcgtat
     6241 cgggaatgtt atcgagatcc atcatacatc gttcctctct tttcttaccg gcacgattac
     6301 ccgtaccggc atcgattaaa atttcagggc gttagcgata tcttcttcgc tctcttcttt
     6361 atcaatcgcg ttttgtgctt tgttagcgac cacaacaaag ggcagataaa ttaacgttgc
     6421 gataccaagg ttgaagagtg cgaccagcaa tgcggcgacg ctaccgttgg tgttaaagaa
     6481 ggctcccaga ccggttggca tggtccacgg tgcaatattg gtcaccggag gaataatgcc
     6541 catgtagtaa gctgcgaggg tgattgccgc cagaatcggt tgtaccagta caaacggaat
     6601 aaacatcacc gggttcatga taattggcag accaaacaga atcggttcgt taatctggaa
     6661 gatgccggac ggcagcgcca gttttgccac ctgacgataa tcagcacggc gagaagcgat
     6721 aaagatagcc aggatcagac ccaaagtcgc accactgcca ccgaggaaaa taaaggagtc
     6781 cagcatcggc ttggcccaga tatggaaggt cttaccggct gccagcgccg cttcgacgga
     6841 accatattgc tgataggtcg cgatgttttc cagcgcccac ggcgtcataa tgccgttgtc
     6901 cagtgcggtc agcgccagcg cgccatgaat accgaagaac cagagcagtg gaacaaagat
     6961 cacataggcc catcccacca cgctacccaa cgatgccagt ggggttgaga tggtatccat
     7021 aatgatctga tggaaattgg taccccaggt attcaacgcc caggcaataa tccccatcac
     7081 ggaaagaata ataaagccgg ggattaatgc cgagaaggaa cgcgatactg aagcaggtac
     7141 gctgtcgggc aatttaatga cccaattgcg gtggacaata aaggtaaaca tttctgccac
     7201 caccaggcca ataataatcc cggagatgat attcgcccca cctaaccagt ttgcgccaac
     7261 cgcataggcc tcaccgacac tgtatggggt gacggtcata aatgctgcaa cggataacaa
     7321 cccagccgcc agcgcatcga ctttacgctc ttctgccagc gccatgccaa taaagaacgg
     7381 tgccattaaa gacattatcc ctaatgttcc gttatatacg ttgccgccaa tacctttcag
     7441 accattaagt gtttcaatgg ttgaggcatc caggcgaata cctaaggaat aaaaaaacga
     7501 cccctcccca aagcttagaa aaacgttgtt aattaataca aacatggccc ccgcaagggt
     7561 taacggcatt aagcgaataa agccattttt gattgcatta acgtgtggct gctttcctat
     7621 tttaactgca aaagggagga gtaccttttc aagcgatgca ataacattac tcatagaaaa
     7681 atacccttaa
//
LOCUS       DACBVI010000189         7635 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.190, whole genome shotgun sequence.
ACCESSION   DACBVI010000189 DACBVI010000000
VERSION     DACBVI010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.190"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            56..343
                     /locus_tag="IU325_20340"
     CDS             56..343
                     /locus_tag="IU325_20340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811351.1"
                     /translation="MLNVNLLFNPLNLPGMGSGVLEEIMSISDSSLRKRLGYEVLSFS
                     LQAHSLSQECIDKLAIFFADDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
     gene            complement(347..1738)
                     /locus_tag="IU325_20345"
     CDS             complement(347..1738)
                     /locus_tag="IU325_20345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HAP5811352.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGLLLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPMDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1871..2461)
                     /locus_tag="IU325_20350"
     CDS             complement(1871..2461)
                     /locus_tag="IU325_20350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP5811353.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2624..3292)
                     /gene="hxpB"
                     /locus_tag="IU325_20355"
     CDS             complement(2624..3292)
                     /gene="hxpB"
                     /locus_tag="IU325_20355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HAP5811354.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3439..3975
                     /locus_tag="IU325_20360"
     CDS             3439..3975
                     /locus_tag="IU325_20360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811355.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4016..4876)
                     /locus_tag="IU325_20365"
     CDS             complement(4016..4876)
                     /locus_tag="IU325_20365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HAP5811356.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGRDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4982..5272)
                     /gene="ghoS"
                     /locus_tag="IU325_20370"
     CDS             complement(4982..5272)
                     /gene="ghoS"
                     /locus_tag="IU325_20370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HAP5811357.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDDG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEIAITTWEEWNSNRK"
     gene            complement(5373..6302)
                     /gene="pfkB"
                     /locus_tag="IU325_20375"
     CDS             complement(5373..6302)
                     /gene="pfkB"
                     /locus_tag="IU325_20375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HAP5811358.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALSEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            6589..7347
                     /locus_tag="IU325_20380"
     CDS             6589..7347
                     /locus_tag="IU325_20380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HAP5811359.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLLGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(7400..7507)
                     /locus_tag="IU325_20385"
     CDS             complement(7400..7507)
                     /locus_tag="IU325_20385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811360.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
BASE COUNT         1974 a         1875 c         1857 g         1929 t
ORIGIN      
        1 ttttgctgcg attcaaaaaa atgagagtct cctaaagttt ccttcggtaa tatcaatgct
       61 taatgtcaat cttcttttta atccactgaa tcttccggga atgggaagtg gtgtgcttga
      121 agagattatg agtatttcag atagttcact tagaaaacgt cttgggtatg aggttttaag
      181 cttctcgcta caagcacatt cattgagtca ggaatgtatc gataagttag ccattttttt
      241 cgctgacgat ctgtttaaat atgagtcagt atgcatcgcc gcgatggaac atttaaaaag
      301 taaggcaacg gcccctatac aaaacggacc gttgccagca taagaattaa cggtgtgcca
      361 gttcggcgtc gtcttcgcta tccagaatgg ctttatcggt ttgcttcagc cactggctgg
      421 tcagcgtgcc agcggtcatc gagccactaa cgtttagcgc cgtacgcccc atgtcgataa
      481 gcggttcaac ggagattaac aatgccacca gcgttacagg caggcccatc gcaggcagca
      541 caatcagtgc ggcgaaagtt gcaccaccac cgacaccggc aacgcctgcg gaactaacgg
      601 taacaatacc gaccagcgtc gcaatccaca tcgggtccat cgggttaatg ccaaccgtag
      661 gcgcaaccat caccgccagc attgccggat acaaaccggc acagccgttc tgaccaatgg
      721 ttgcaccgaa agaggcggcg aaactggcga tggattcagg aacacccaga cgacgcgttt
      781 gtgcttccac attcagtggg atagacgcag cactggaacg gctggtaaag gcaaacgtca
      841 gaactggcca taccttacgg aagtacttca gcgggctcac gccattaata cccagcagaa
      901 ggccatgcac tgcaaacata atcagaagac cgaggtagga cgcgacaacg aaacttccca
      961 gtttgatgat gtcttgcagg ttagaacctg caaccacttt ggtcatcagc gccagaacgc
     1021 cgtaaggggt cagctgcatg accaggcgaa ccagtttcat cacccagctt tgcagggtat
     1081 cgatagcggt taagacgcgt tcacctttcg gcgcatcatc cttcagcagc ttcagcgcag
     1141 ctacgccgag gaatgcggca aaaattacca cgctgataat tgacgtcgga ttggctccgg
     1201 tcagatcggc aaacgggttt ttcgggataa aggacaagac cagctgcgga acgctcaggt
     1261 cagagacttt accaacatag ttactttcaa tggcgttcag acgtgcagtt tctgcaccac
     1321 cctgaaccag accttcagcc gtcaaaccaa acaggttggt gaccagcaca ccgaccagcg
     1381 ccgcaatcag cgtggtaaac aaaagcgtac cgatggtcag aaaactgatt ttgcctaact
     1441 gagatgcgtt atgcagacgg gcaaccgcgc tcagaataga ggcgaagact aacggcataa
     1501 cgatcatttg cagcagttga acatagccgt taccaacaat gttaaaccac tgtacagaat
     1561 ctttaagtac ctggctgtca gaaccataaa tggtatgcag ggcgaggcca aaaaccacac
     1621 ccatcaccag acccaccagc acttttttcg ccagactcca ctgtttatgg cgggtctgag
     1681 ccagcgcaaa cagcagtaca acgaacacca cgatgttcgc aattaatgga aagttcatcc
     1741 ccgttctcct gatcttatta tttctatcac tgcgtagcag tcatagtgtt ggcgcaagat
     1801 taacagaact gtgagctggc tcttatattc aaatggaatt gcttattcca gaaaggtgat
     1861 ttttgccttt ttagtattca acctgatgct taataagccg atgaaactgg atctgcaaag
     1921 tattgatcat ttgcgatgac caacgaacag cgctgttttc gggtaatgaa tggggcatcc
     1981 ataccgacca gacaaataat gccatgcaga taaaacgttc cagttggttg cccttttggg
     2041 gaaccaggat aggcaagcgg aaacgccagc ggcatggcca gagcagggga acaccggcgg
     2101 gtgtcagcat atcggcaagt atgtggctca aataacccag caccattcct tgtagcgcat
     2161 cagccggaat gaaccagctt tcaggaacct taaggtaaaa ggttgccagc agcgcaaata
     2221 ccgccagcag actgtgggta aaaccacgat gcccaaaagc gcgggcgatc ggttttgata
     2281 tccactttaa tcgctgccca agaaacgact ttgggtgatc gatgtccggt aacaaacacg
     2341 tcaggattgc ggaagggaca atatgccacc agtcaccctg tgccagcacg ggcgtcagct
     2401 cggcattttt ggcaaatacc gcacaagcaa tagaaaagag aaggtgacct tccgccgtca
     2461 tgataaaact cactaaactg tcaatacgta cagtatagga tttttataca gtgcacggaa
     2521 gtggtgacgg tgtaactctt tctcttaaat tgacgattaa tccgtcagtt gaatgcaaat
     2581 tcagataaag aaccaggagc agtgtgatca ctgcccctgg tcattaaccg agaaggtctt
     2641 ttgcggtgag ttctgtcagc gatgagagtt tgacgtttgc taatacaaaa cgtggatcat
     2701 tttgcgcttc tggcgcagga acgacgatgg aacgcatgcg ggctgcttta gaagcgatca
     2761 tgccatttac cgagtcttcc agcgccacgc aggtcagtgg gtcaacgccc agttttgctg
     2821 cgcagtcgag atatacttgc ggatgcggct tgctgtaagg cagtttttca gccgaggcga
     2881 gggcatcgaa actgtcgcgt aagtcaaaca tggtcaacac tttttccagc atatgtagtg
     2941 gtgacgcgga ggccagtccc actaataaac cttgttcttt gcataacgca acagcttcgc
     3001 gcacgcctgg taataatgga cgcgtctctt caaccagtga aatggcacga gcaataaccc
     3061 gttctactac ttcctgacgg cttggcccat tccagggttg ccgggcgtac caaagatcga
     3121 ccaccatatc gatgcgtaaa cctaaggtgt ccggcagctc gttacgacgg gagatatcca
     3181 cccccaggct tgccatcaca tccagttcag ctcgatccca taaaggttct gagtcgataa
     3241 gtaatccatc catatcaaaa attgcagcaa gaatctgacg cggggttgac attacaacct
     3301 ctcctttaga gcattatgtg taaagtatta agccgtgctt atgattaagc gcctatcata
     3361 tcgcctttaa agaatgggcg aaaacagata tctacaggta aactaagtga aacgacgcgt
     3421 cttaatcaag gagaactcat gacgtatcaa caagctggac gcattgctgt tttgaaaagg
     3481 attttgggtt gggtgatttt tatacctgca ttgatctcta cactgatttc tttgctgaaa
     3541 tttatgaata cccggcagga aaaccaggaa ggcattaatg cggtcatgct cgactttact
     3601 catgtcatga tcgatatgat gcaggcgaat acgccttttt tgaatctgtt ctggtataac
     3661 tccccgacgc ccaattttaa tggtggcgtg aacgttatgt tctgggtgat tttcatcctg
     3721 atttttgtcg gactggcgtt gcaggattcc ggtgcccgga tgagtcgcca ggcgcgcttc
     3781 ttgcgggaag gtgttgaaga tcaactcatt ctggaaaaag ccaaagggga agaggggcta
     3841 acgcgtgaac aaattgagtc acgtattgtt gtgccgcacc atactatttt cctgcagttt
     3901 ttctcgctgt atatcctgcc ggttatttgt attgctgcag gttatgtgtt cttttctctg
     3961 ctggggttta tttaatcaaa aggccgcacg gagcggccat tatcctcacc ccatattatg
     4021 ctgctaataa tctatccagt gactgctgag caataaccaa atgctgaccg ccaaataacc
     4081 ttgcacgatt taacagcgtg tagagttggt aaaccggttg acgttcaagg aaatcggcag
     4141 gtagcgggga tactgactga tagccgtcat agatttgtgg cggctgttca gtatgcagcg
     4201 gtaacatcgc caggtcgcac tctcggtcac cccagtagca ggccgggtcg aaaatgtacg
     4261 ggccatccgg acccagcgca cagttgccgg accataaatc gccatgcaac agagaaggct
     4321 gcggttgatg cgaggccaga cgctgctgaa tatgctctac gatggcgtcg atattgccga
     4381 aagcgatccc tttctctgct gctagttcca actgccagcc aatccgttgt tcagcaaaaa
     4441 acgttgacca gcgacgttgc caggtgttgg gctgcggagt tgtggagagc gagttatcaa
     4501 aatcgaggcc aaattgtggt tggtcgctcc attgatgtaa acgcgcaatt tgctgaccaa
     4561 gaataaatgc gctatgcgca tccagcgggc gaggtgggag ataatccatc accagaaaac
     4621 tgtagtcacg gtcagcgcca actgcccaaa ccttaggcac ggtgacggtt ttactacgcg
     4681 acagtaactc cagttggtcg gcttcggcgg taaaaccggg aagcagttcc ctttcatcac
     4741 atttgacgaa aaagtcacgt cctgcatagc gcaaatgcca tgcggcatgg acttctccgc
     4801 caggcagttc attacgcagt tcgatttcgc cttcacctaa ctgctcgctc aaaagacgac
     4861 tgattgcctg ccacatgatg tctctcccat gttgtctgcc agatcataag gttagcgcaa
     4921 gaatgcggtg taaaaacacg agctaacgca caccacagac tgattattgc ccaaaaacca
     4981 tttattttct gttgctattc cattcctccc aggtcgtgat ggcgatttca ggatctttac
     5041 cggttgcact ttgggtaagc cccgaaacca gttctctaat ttcttcttca ctttgggtac
     5101 tgataagtcc aaaagtgtta gtccccagct catggatatt tccatcatca tccgtcatgg
     5161 tgagcaaaaa accgtcgcga gtcaggtaat tattcaactc gttaatttca gtcaatgtat
     5221 cctcatgcaa catgacggtt atgacataac ggacaagatc gccactggcc atagttcacc
     5281 cctttgttat cacatgactt ttgagcatag tcggagaaac gcgttgccga caggttggtg
     5341 gtgattcccc caatgcaggg ggaatgtttc agttagcggg aaaggtaagc gtaaattttt
     5401 tgtgtatcgt catgggagca caggcgtgtt ccctgattga gtgtggctgc actccccgca
     5461 gctacgccaa aacggaccat ctcttcaaga gaggcatttt ctgccagttt cagtgtcatc
     5521 gcgccgacca tgctgtcacc agcgccaacg gtgctctggc ttttcaccgg tggtggcaca
     5581 acctgaatgc agttttcact atcaacgccc agcgcccctt gtggacccag ggaaacgaca
     5641 actcgtttgg ccttgccgct attaacgatt tcctgcgcgg ctttgcggac atcgtctggc
     5701 tgggtgagtt cgcgattcac tagcgcactg agttcttttt ggttaggctt aaccaactcg
     5761 atattaccaa tcgccagtgc tgcacttaac gcttcgccag aactgtcgac gatgcagcga
     5821 atcccttgtt tttgcgcagc ggaaatcagt tgggttaatt tttccagctt cacacctggc
     5881 ggcaggcttc cgcttatgac caggatagca ccggattcaa tttccagaac ttgctcttca
     5941 agctggcgaa actcatcttc acttaatgcc gcgcctggca tgacaaaacg atactgctca
     6001 ccgcttgctt ccacatgtac gtgtaaattc tgccgggtcc agtctttggc ttctacagta
     6061 gcgacgggga cattttcatc tgccaacagt gaaaccagat gttcgccggt cgcaccaccc
     6121 gccgggaaga tcgctgtggc actgcctcca agatgggcaa tggcgcgggc gacgttgatg
     6181 ccgccgcctc cgggttcgaa taccggtgcg gtacagcgca gctttccttc gggataaatt
     6241 tgcggggtaa ttgttgcgct atcgagagag ggcgcaagtg tcaacgtata gatacgtacc
     6301 atcatttcct cctataggct gatttcagtc tggcaccgaa tcagcggaaa gggaagaaat
     6361 taacaatatg atttattaga gctttattta aaattttgca gataaatata tataaataaa
     6421 aatctgtttt cttcacatgc ttattcaaaa aatgaaataa gaatacattg ctaagttatt
     6481 caagcctttt tataattcgt tacctttcca gggacgctaa tctttgatcc ttaagaaagt
     6541 ttccgggacc gcgatggtct ctttttttgt tgtacggact cttgataaat gaagcttttg
     6601 aagacagttc ctgccatcgt tatgttggcg gggggcatgt ttgcgtcatt gaatgcagct
     6661 gccgatgatt ccgtttttac tgtcatggac gatcctgcca gcgctaaaaa acctttcgaa
     6721 ggtaatctga acgcaggata tcttgcacaa tcaggcaata cgaaaagctc ctcacttacg
     6781 gctgacacca ccatgacctg gtatggacaa accactgcct ggtcgctgtt ggggaacgcc
     6841 agtaatactt cttctaatga tgagcgttct tcagaaaaat atgccgcggg cgggcgtagc
     6901 cgttttaatt taactgatta tgactattta tttgggcagg caagctggct gacagaccgt
     6961 tataacggct atcgagagcg cgatgtgttg accgcgggtt atggtcgtca gtttttgaat
     7021 ggcccggtac acagtttccg ctttgaattc ggtccaggtg tgcgttacga caaatatacc
     7081 gacaatgcca gtgaaaccca gccgctgggc tatgcctccg gggcttatgc atggcagttg
     7141 actgacaacg ctaaatttac gcagggtgtt tcggtcttcg gcgcggaaga cacaacgctg
     7201 aactccgaaa gcgcattaaa tgttgctatt aatgaacatt ttggactgaa ggtggcttac
     7261 aacgtaacat ggaactctga acctccagag tctgcaccgg aacataccga tcgtcggaca
     7321 accttatcgc tgggctacag catgtaaaat tatctgccgg accgtgttat gcggtctggc
     7381 tgtcttcgtt tgtcactgct cagccccaaa tcattgctgc ccaacgtagc aacaacatag
     7441 cgccacagat tgccagtagc accaccacca tcttccagtg ttttttatca aagcgtttag
     7501 tcggcatacc agtgtcctgc atatctcttg tgcgtgataa cggagtgtaa caaaagggac
     7561 aggcatcaag ggattttttg ttattacagc cgtcgggtgg cgttgtacac ctgatcactg
     7621 tttttgcggt ttatg
//
LOCUS       DACBVI010000190         7630 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.191, whole genome shotgun sequence.
ACCESSION   DACBVI010000190 DACBVI010000000
VERSION     DACBVI010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.191"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(159..1295)
                     /locus_tag="IU325_20390"
                     /pseudo
     CDS             complement(159..1295)
                     /locus_tag="IU325_20390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015878551.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(1707..2313)
                     /locus_tag="IU325_20395"
                     /pseudo
     CDS             complement(1707..2313)
                     /locus_tag="IU325_20395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
     gene            2580..4079
                     /gene="ansP"
                     /locus_tag="IU325_20400"
     CDS             2580..4079
                     /gene="ansP"
                     /locus_tag="IU325_20400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HAP5811361.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEQQEIVFKPETAS"
     gene            complement(4192..5253)
                     /locus_tag="IU325_20405"
     CDS             complement(4192..5253)
                     /locus_tag="IU325_20405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HAP5811362.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            5495..7597
                     /locus_tag="IU325_20410"
     CDS             5495..7597
                     /locus_tag="IU325_20410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HAP5811363.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTSVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVITQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGAMGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTEAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPLTFTTGLNYENMSENRKGYNNFRLNSGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTSGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVKA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGMNIAWRFE"
BASE COUNT         1879 a         1873 c         1832 g         2046 t
ORIGIN      
        1 aaaaatcgat tactttatgt cgcatgacaa atatgaatta catattaatc aaccttaaaa
       61 acaatcatct cttataatta acagcatacc atgagaaaca caaaatcaat ttaccatgca
      121 taccaggagc ataattaacc tctcaccgca gggcgaaatc atgaaggccc gcagccagca
      181 aggattagag aaaggtattc attcttacgt cctgcccgct tttgcttgcg tttaataccc
      241 gctttaaagc tattctctgc ggtcagtaaa ttcatcgcga tatgtcgaca aactgccaga
      301 ttttctcccg cttctccacg aacaatctgg cattcatctt cccgcatccc catatcaagt
      361 cgccagtgca gttgattttc taccgaccag tgtgctcgtg ttgcttccag gagttctttc
      421 gccgtaagcg tcaccgaact tatgtagtag cgaagaatac acttctctga tttcaccgct
      481 gtttctcctt cctgtctgct cgccagcact attcctatcg tgctcagctc tgaccattca
      541 taagctaaat cacccagagc ggttaaatca tggctcacaa tgcagcatcg tgtatcttcc
      601 cgtccatgcc ctttttcctg cgtgctgtaa ctgtcatacc tctcacccag ccacattgtc
      661 ggcgagtacc cgttatcaaa cgcctccgct aattttccct gatttccttt cagcgccagc
      721 aggtaaccgg ctttcttttt cagaatttca cgggcgatta tatgctggca acccattgca
      781 tccagcgtga ccagacaccc ttttaattcc agcaactgga gccgttccgg gatcgccgtt
      841 atctcattcg attttgcccc tgttttcacc tgccccaaaa ccagacgatt ttctgcgcta
      901 aaagcactga ccctatggat cacggcatta cgtttcgatt tatcgtaagt accccgtaat
      961 gacttaccat caatcgccac aacagagcct tcagacaacg tgtgacagtc cttcatctac
     1021 tggataaaaa ggggctgtaa ttgttttgct gaaatcattc ccatcactcg ggcaatcgtg
     1081 tgatgggagg gggtgccatt ttcaaaatca ccatacaggc gtaaccattc cagatgctcc
     1141 tcaccaaaat cagcaatttc ttcccatcct tcagcgccac cgataatggc ggtgatcgtc
     1201 agaaaaagga tatccgccag gctgtaactg gttttccagg actggcgata atcgtgaata
     1261 attgaaattt gatccaaaag gcttagtccg ttcatggtgt gctatccatc aaaaagaaag
     1321 gataagatca cactgaaata agatggtcaa attgatcgaa aaaaaagatc aacatgatgg
     1381 gttttacgat aaaagtaatg tcgcgattta aaaattcatc attcaccaaa gtcttgtggg
     1441 gcgcgggctt tcatgctttt tccctgatac gctaccgctc tgccctgata acatgatctg
     1501 cacggcgcta tcatatcgac atatttctct acatgcgaaa ggatgtttta ttttcggatt
     1561 atattaaatt gatatttcat ataaaaaacg agattacata aatcatataa ataattgtac
     1621 caactttaat taccattcat agcaaacaaa tggatgtcca gttaactacg ccagtgattt
     1681 attaacatta atctccggca ttattgttaa ataatggcat tcctttttag tatttcttgt
     1741 aacgttggta tcggacatac agcatcagca atcgcactga tattcgcggc attatcttga
     1801 aaccattcat gccccggtcc ccatatgcgc ataacgcaga gataagggta gctgatcgcc
     1861 aaacgcataa ggttcagcag tgagctacga attcaaccag atataaaacg attttcgata
     1921 ttcaataaca ttcttcttta actgttcggg tgcgtcagga gcccaacgct cagggtaatc
     1981 ggcgaaagtg aatgttggat agacattagc aaccagccag accaatagcc gttgaaactg
     2041 ctgacgttcg gcgcgcccaa ccggtggcgc aaggtccggg cgacgatcga gaaccatcaa
     2101 tgcaatcgcc gctgtctccg tcataatctc gtcattttcc agcgccagtg tcggaatctg
     2161 acacaacggg ttcagggttt tcaataactc gcgtgaggtt ccctcgtggt caaaaccact
     2221 gacatcaacg aactgataag gaatatcagc cagtgttagc atcagctcac tgattgtaga
     2281 gccccatccg ggtacgccat acaccttaat catatgcccc tcacaatctt ttaaagatta
     2341 agtgtaggac agtacacagg ttaaccagca aaaggatttg tcattttttc gtcagttgta
     2401 ttattcccct tcaggattgt aaggggatgt tttgcatcgt ttatcgataa cgggtaggat
     2461 gctgcgccga tagttttata cccataaaga ataatggtga taactatcat cgccaggatg
     2521 aataaacatt gttcatggca acttatatga ctttttcatt aaagcaatca gggagagcaa
     2581 tgagtaaaca cgacaccgac acttcagatc aacacgccgc gaaacgccgc tggcttaatg
     2641 cccacgaaga ggggtatcac aaagcgatgg gcaatcgcca ggtacagatg atcgccattg
     2701 gcggcgcgat tggtacgggt ttgtttttag gcgcgggagc ccgactgcaa atggcggggc
     2761 cagcactggc actggtctat ttgatctgcg gcctgttttc attttttatt ctgcgtgcat
     2821 tgggtgagct ggtgttacac cgcccttcca gtggcagttt tgtctcttat gcccgtgagt
     2881 ttttaggaga gaaagccgct tacgttgcag gctggatgta cttcatcaac tgggcgatga
     2941 cggggattgt cgatattacc gccgtcgctc tgtatatgca ttactggggt gcgtttggcg
     3001 gcgtgccgca gtgggtcttt gcgctcgctg cgctgaccat cgttggcacc atgaatatga
     3061 tcggtgtgaa atggtttgcc gagatggagt tctggtttgc gcttattaaa gtgctcgcca
     3121 ttgttacctt tttggtcgtg ggtacagtgt tcctcggtag tggtcagccg ctggatggca
     3181 acaccactgg ttttcattta attaccgata acggcggctt cttcccccac ggtttgctgc
     3241 ctgcgctggt gttgattcag ggcgtagtgt ttgcttttgc ctccattgaa atggtgggta
     3301 cagctgccgg agaatgtaaa gatccgcaga ccatggtgcc taaagccatt aacagtgtga
     3361 tttggcgtat tggcctgttt tacgtcggct ccgtggtgtt gctggttatg ttattgccgt
     3421 ggagcgcata tcaggcgggg caaagtccgt tcgtgacgtt tttctctaaa ctgggtgtgc
     3481 catatatcgg cagcattatg aacattgtgg tgctgaccgc tgccctctcc agcctgaact
     3541 caggtctgta ctgcaccgga cgtattctgc gctcaatggc gatgggcggt tccgcaccga
     3601 gttttatggc gaaaatgagt cgtcagcatg tgccgtatgc cgggattctg gcgacactgg
     3661 ttgtgtatgt cgtcggcgta ttcctcaact atctggtgcc gtcgcgcgta tttgagattg
     3721 tgttgaactt cgcgtcgctg ggaatcatcg cctcatgggc gtttatcatc gtgtgccaga
     3781 tgcgcctgcg taaagcgatt aaagaaggca aagccgcgga tgtcagtttt aaactgcctg
     3841 gcgcgccctt cacttcctgg ctgacattac tgtttttgct gagcgtcctg gtgctgatgg
     3901 cgttcgatta cccgaacggg acttatacca tcgcggcgct gccgattatc ggtattctgc
     3961 tggttatagg ctggtttggt gtgcgcaaac gcgttgctga aattcacagc actgcgccag
     4021 tcgtcgagga agatgaagaa caacaggaaa ttgtgtttaa gcctgaaacg gcgagttaat
     4081 tgcaaaattg cccggctacg tgccgggcaa attcaaatta ctgacgaagg tgccggatac
     4141 ggcgtgataa aagtcaggca agtctcaccg ttttcaccct cgccatatct attacagcgc
     4201 aatacgaatc acatcatccg gctgggttgc ttcctgctgt ttagtggatt tttgtttcac
     4261 actgacatac agcgttttac catcggcaga cagcgccagg ctgttcggat gagtcggcgt
     4321 atcgaacgtt ttcaccactt tatagctttt cgcgtcaatc acactgactt tacctgcctg
     4381 acgatgcgtt acgtaggctt cattacgcgc cgggttaaac agcacagcca gtgattccgg
     4441 tgccgcaacc ttcgccagaa tattgccatt acgggtatcg accactaaca cttctgcggc
     4501 tttagaatcg gtgataaatg cacgctgcct ggcggtatca aggctaatgt tgataaagaa
     4561 gtgctctttg ccgtcatcca gcagcttttt acggctgagg attttattgt cggcggtgtc
     4621 gatggtaatc aattcgccgt cagcgttagt ggtgtaaaga cgtttgcctt tgctatccag
     4681 cgccagaccg gtactcattt taccggtgtt ctggatggcg gttttcagtt tgatattctc
     4741 gccatcaacg acccaaatca cgctctcttt accaatacca ctgatgtaaa cggtgttcgt
     4801 ggcatcatca gctaccagct cacgcggttg cagcgggcgc acctcttccg tgcgcttacg
     4861 atcatccagc accagacggc ctttcacttc gcccgttttg gcatctatcg ccgtgaccgc
     4921 gctgtttaca gtgttaccaa accacaacgt ctgagtcgtg ttattgatgg tggcaccaaa
     4981 cggcttgaga tcgttatgga tcgcctgcgt cacttccaga gtaaccggat caagacgata
     5041 aaccacgccg cctttatcca gtttgcggct ttgcgaagtg gcgagccaca gcgcgttctc
     5101 ctgctggcta taagccattt cgtaggcacc tttacctacc gctttacgca gcatttcttc
     5161 tgcagcctgc gtactgaatg atgaagcaac aagcaatgaa cctaacagta atgaaccacg
     5221 caggcgcgat gaaaacagat gacgtaaatg catgacgact ccctttgatg aaccgatatt
     5281 tttgctgctt cacaatgctt cgctaagcgt aatcatcgtt acgctcttgt tataaatggg
     5341 aatggtaatc attattttct caaatgtgga gcatttcttg ccgttacgac tacaattttc
     5401 tcaattactt aacgtatcgt gccgtttcgt tttccatata tttacaattt tattaccatg
     5461 gcggcgtttt cccattctta acttggcttt tttcatgaag attttttccg tccgacagac
     5521 cgttttgccc gcactgcttg tcctttcccc cgttgttttt gccgctgatg aacagaccat
     5581 gattgtcagt gccgcaccgc aggtggtttc agaactggat accccagcag ccgtgagcgt
     5641 ggtggatggc gaggagatgc gcctggcaac accgcgcatt aacttgtccg aatcactgac
     5701 cagcgtgcct ggtttgcagg tacaaaaccg gcagaactat gcgcaagatt tacagctgtc
     5761 gattcgcgga tttggctccc gctccactta cggaattcgc ggtattcgcc tgtatgtgga
     5821 cggtattccc gccaccatgc ccgacggaca agggcaaaca tccaacatcg atttaagcag
     5881 tgtacaaaat gtggaagtgc tgcgcggccc cttctctgct ctgtatggca acgcgtctgg
     5941 cggtgtaatg aatgtcatca cccagaccgg acaacagcca ccaaccattg aagccagtag
     6001 ttactacggc agttttggca gctggcgcta tgggctgaaa gcaacaggcg caatgggcga
     6061 cggcactcag cctggcgatg tcgattacac cgtctcaacc acgcgcttta cgacccacgg
     6121 ctatcgtgat catagtggcg cacagaaaaa tttagccaat gccaaactgg gcgtacgcat
     6181 tgatgacgcc agcaaattaa gcctgatttt caatagtgtg gatatcaaag cagatgaccc
     6241 aggtgggcta accgaagcag aatggaaggc gaatccgcaa caagcgccac gcgctgaaca
     6301 gtacgacacg cgaaaaacca tcaagcaaac tcaggctggg ttgcgctatg aacgtagcct
     6361 gagcgcacaa gatgatatga gtgtgatgat gtatgccggg gagcgagaaa cgacccagta
     6421 ccagtcaatc ccgatggcac cacaacttaa cccgtcgcat gcgggcggcg tgattactct
     6481 gcaacgccat tatcagggga tagacagccg ctggacacac cgtggcgagc tgggcgttcc
     6541 gctcacgttc actaccggcc tgaactacga aaacatgagt gaaaatcgca aaggctacaa
     6601 taacttccgc ctgaatagcg gcgtgccgga atacgggcaa aaaggtgagt tgcgccgtga
     6661 cgaacgcaat ctgatgtgga acgtcgatcc ctatttgcag acacagtggc agctgagcga
     6721 aaaactgtcg ctggatgctg gcgtgcgcta cagctccgta tggtttgatt ccaacgacca
     6781 ttacgttact tcgggtaacg gcgatgacag cggtgatgcc agttatcaca aatggctacc
     6841 tgccggttcg ttaaaatatg caatgaccga tgcctggaat atctatctgg cagccgggcg
     6901 aggttttgaa acgccgacga ttaatgagct gtcttatcgt gctgacggtc aaagcggtat
     6961 gaactttggt ttaaaaccat ccaccaacga tacaattgag atcggcagta aaacgcgtat
     7021 tggtgatggg ctgcttagtc tcgcattgtt ccagaccgac accgatgatg aaattgtagt
     7081 cgatagcagt agcggtgggc gtacgactta caaaaatgcc ggaaagaccc gtcgtcaagg
     7141 cgctgagctg gcatgggatc aacgtttcgc gggagatttt cgtgtaaaag cgtcctggac
     7201 ctggctcgat gcgacctatc gcagcaatgt ttgcaatgaa caggattgta acggtaatcg
     7261 gatgccaggg atcgcccgta atatgggctt tgcgtcgata ggttatgtac cggaggatgg
     7321 ctggtatgca ggtacggaag cgcgttatat gggcgatatt atggcagatg atgaaaatac
     7381 ggccaaagcg ccgtcttata ctctcgtcgg cttattcacc gggtataaat acaattacca
     7441 caatttaact gtggatttat ttggtcgtgt cgataattta ttcgataaag aatacgttgg
     7501 ttctgtcatt gtcaatgagt caaacgggcg atattacgaa cctgcgcccg ggcgaaatta
     7561 tggtgtcggc atgaatattg catggcgatt tgagtaatta ccttgatgcc cggtatttgc
     7621 cgggcattta
//
LOCUS       DACBVI010000191         7497 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.192, whole genome shotgun sequence.
ACCESSION   DACBVI010000191 DACBVI010000000
VERSION     DACBVI010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.192"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..499
                     /locus_tag="IU325_20415"
     CDS             <1..499
                     /locus_tag="IU325_20415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAP5811364.1"
                     /translation="VHASELVGSLLKSSRPSGLAQAIMEVGRVNKTLYLLNYIDDEEY
                     RRRILTQLNRGEGRHAVARAICYGQRGEIRKRYREGQEDQLGALGLVTNAVVLWNTLY
                     MQEALSHLRSAGEIPEDEHISRLSPLMYGHINMLGHYTFTLPENILKGELRPLNFNSN
                     NELLP"
     gene            535..3441
                     /locus_tag="IU325_20420"
                     /pseudo
     CDS             535..3441
                     /locus_tag="IU325_20420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element IS3000 family transposase"
     gene            3982..4857
                     /locus_tag="IU325_20425"
     CDS             3982..4857
                     /locus_tag="IU325_20425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase
                     CTX-M-15"
                     /protein_id="HAP5811365.1"
                     /translation="MVKKSLRQFTLMATATVTLLLGSVPLYAQTADVQQKLAELERQS
                     GGRLGVALINTADNSQILYRADERFAMCSTSKVMAAAAVLKKSESEPNLLNQRVEIKK
                     SDLVNYNPIAEKHVNGTMSLAELSAAALQYSDNVAMNKLIAHVGGPASVTAFARQLGD
                     ETFRLDRTEPTLNTAIPGDPRDTTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNT
                     TGAASIQAGLPASWVVGDKTGSGGYGTTNDIAVIWPKDRAPLILVTYFTQPQPKAESR
                     RDVLASAAKIVTDGL"
     gene            complement(4904..5236)
                     /locus_tag="IU325_20430"
     CDS             complement(4904..5236)
                     /locus_tag="IU325_20430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin fold metalloprotein, WbuC family"
                     /protein_id="HAP5811366.1"
                     /translation="MRPHRHPHTFELLLPLRGRFVVLNFDDRGTVTHRAILGETCTVL
                     EMAAGTWHAVLSLDTGGIIFEVKHGGYQPVAADDYAHWAPAEGEPGTTELMAWYAQAQ
                     VGDSTFAV"
     gene            5250..7497
                     /locus_tag="IU325_20435"
                     /pseudo
     CDS             5250..7497
                     /locus_tag="IU325_20435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496645.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
BASE COUNT         1949 a         1884 c         1950 g         1714 t
ORIGIN      
        1 agtccacgcc tcagaacttg tgggctcgct tttaaaaagt tcccgtccgt cagggctggc
       61 gcaggcgatt atggaggtgg gacgggtcaa caagacgctg tacctcctca actacattga
      121 tgatgaagaa tatcgtcgca ggatactgac tcagcttaac cggggagaag gccgtcacgc
      181 cgtggcaagg gcgatctgtt acggccaacg tggtgagatt agaaaacgtt accgtgaggg
      241 gcaggaggat caactgggtg cgctggggct tgtcactaac gcagttgtat tgtggaacac
      301 gctttatatg caggaagcgc tatcacattt gcgcagcgct ggtgagatcc cggaagacga
      361 gcatatctca cgcctgtcgc cactgatgta cggtcatatc aacatgctgg gacattatac
      421 gttcacgctg ccggaaaata ttctgaaggg agagttgagg ccattaaatt tcaattcaaa
      481 caatgaatta ttgccttaac gtggtttttt acacgattga acctcgaacc ccataaggaa
      541 ctggcgatcg ctaaaagcct ccgtcatcgg ggccagcgtt acatgctggt ggtactgctg
      601 ggctatttca aagccaagcc ggtggtgctg aatcccggct ttcatcagat caagcaggac
      661 ctcaaatacg tttatcaaac cgttctacca ggcccaggct gtagaccctt taatctcacg
      721 ccaaaagaga acgaacggat ttaccaacgt gttttccagc tctgcaacta ccagcgctgg
      781 aatgtcaaag accatggagc ggcgttgaga gattacttat cccagcaagc cagagcctgg
      841 acagcgccta ggcacctctt cgatgcggcc aatgaatatt gttcggggca gaagatcgca
      901 atccctgcct acagcacgct gcaaaagatc atcagtcagg tggtgggaga cgaacaggaa
      961 cacatggccg cgcaccttga gcgtgcaatg tcacgcggtc ttaagcaagc actggcggag
     1021 ctcgtgaacg gcacaggtcc actgccgttc cgacagttgc gacaatcggc tcgtaacttc
     1081 accggaaccg agttggagaa agaactgatt gtctatcgcc atatccagca ttggatgccg
     1141 gaagtggatc tgctgttgag cacgctatca ctgtcgcaga aaaatctgca acacctggca
     1201 gagaaagtcg actactacgg tgccaaactg aaacgtcaaa ccgtaggcag ccaatggctg
     1261 tatttattgt gctatcttca aacgcgatgg cagcaagcgc tggaacgtat tgccgatggc
     1321 tttgtgcatc atgtcagaca gaccaaacag aaggcaaagg attatgcgca ggaagcggtg
     1381 tttaaggatt ggcaaaaagc agctaaaaat gtcagcaagg cagctgaagt actgcacctg
     1441 ttcattgatg acagcattga tctgcaacta ccgtttgcaa cagtaagaca gcaagcactg
     1501 agcctgctga ccaaaaggga tctggaatct gtatgtctct tcctgaacga gcagcgacga
     1561 tcggtcgatg aagccatgtg gcagtactgc gacgagaaag aaagtctgcg gaaaggtttg
     1621 ctgcgagagt tgttcctatg tctgcgcttc gaaggctgcg acggcaccca gcacttagcg
     1681 gccgccttgg cgaaaacaca aaacgaactc aacggccagg acgctcagtt gcaaactgcc
     1741 gacaccagac tcctttccaa aaaatcacgt gaattcctgc tggatggtga agggaatatc
     1801 ctgatcgatc gttatgagtg gttcctctat caacagattc ctgatcgcct gaatggccag
     1861 ctgacgctgc ctgatatcac taaataccgg gcactcgacg ccgacctgat cgacggggaa
     1921 cattggcgaa aaaacaaata tacgctgctt caacagagcc attttacaaa attagcggag
     1981 gagcctgaaa agctgatcaa acagatggcc atggaattgg atacccgttt gtacgaagtc
     2041 ggcgaatatc ttgaacagga agacaaccgg aatatcatct tgcgtaatcc gcagggtaaa
     2101 cgcttctggc gcctgccttc ggccagcaaa catcatctgg tcaacaatcc cttcttccag
     2161 caaattccca caacggggat tgcggatgta ctgcgcatgg ttgatcgtga caccggtttc
     2221 attgactgct tcgctcatgt gctgggttcc caatccagaa gccgttccca tgaatatgac
     2281 ctgttggcaa ttctggtcgg caatgcaacc aatcaaggca tttacggcat ggcacagatc
     2341 tctgatcgta cctatgatca gctcagcact atccaggcga actatcttcg cctggaaaca
     2401 ttgaatgctg ctaacgacaa catcaataac gcgacagcca agctacccat cttccggtac
     2461 tacaacatcc aggaagatgt gatccacgcc agtgccgatg gtcaaaagtt cgaagcccgg
     2521 cgcgagacct tcaaaacccg ttattcgtcg aagtactttg gcactcaaaa aggtgtttct
     2581 gccatgacct tgatcgccaa tcacgctgcg atcaacgcca gagtgatcgg cgccaacgaa
     2641 catgaatcgc actacatctt tgatttgttg atgagcaata cgtcagacat cattccggat
     2701 gtgctctcaa ccgataccca tggggtgaac catgtgaact tcgcgttact ggatctgttc
     2761 ggataccagt ttgccccacg ctatgcccag gttggcaaag tgatcaatga catgtttgat
     2821 gtcaaggaag acaaagaaca ccgaattcag ctgtgcttaa aaaagccaat caatacccat
     2881 cgtattgcgc agcactggga taccatccaa cggattgcag tatcacttaa gcagcggaaa
     2941 acaacgcaag ccaccttggt gagaaagctc tcggagtaca agcgcaatca cccgctgctg
     3001 gaagccctga cggaatacaa tcgcctggtg aaagcgaatt atctactgtg ctacatcgat
     3061 gatgccagtt taagaaacta tgttcagcgc gcgctgaacc ggggagaggc ctatcaccaa
     3121 ctgcgtcggg ccgtgagcag cgtcaatggg gatcagttcc ggggcagttc agacgaagaa
     3181 atccagctat ggaatgagtg cgctcgcctg gtcaccaatg ccatcgttta cttcaactcc
     3241 aggatactca gccagctgtt gaccagcttc gaataccaag gagataccaa gagaatagat
     3301 atcgtcaaac aggcatcccc tgtggcctgg cacaacatta acctcaaggg gacttaccac
     3361 ttcgaattga gcgaaaaatt gccagatctg gaggagctta tgcgctcaat cgagggatat
     3421 ttacccgtca gcgaaaagta atacccctct ggaggccacg gacggcgcgg cctccagagc
     3481 actttgtcgt ttttggacgg aaaatcccta gaaccccatc ttcaaatctc ccccagtacg
     3541 gtgagcgttt acctaaagcg tgatcgtccg tgatcgaaac aagccttatc agataatagc
     3601 cccctttagg ggggttcctg cgctcttgtc ttttagcctt aaaaaatcct tttagcgcgg
     3661 atctttggca tgaagttgtc ggaaaactat ccgtacaagg gagtgtatga aaaatgtctg
     3721 gtataataag aatatcatca ataaaattga gtgttgctct gtggataact tgcagagttt
     3781 attaagtatc attgcagcaa agatgaaatc aatgatttat caaaaatgat tgaaaggtgg
     3841 ttgtaaataa tgttacaatg tgtgagaagc agtctaaatt cttcgtgaaa tagtgatttt
     3901 tgaagctaat aaaaaacaca cgtggaattt agggactatt catgttgttg ttatttcgta
     3961 tcttccagaa taaggaatcc catggttaaa aaatcactgc gccagttcac gctgatggcg
     4021 acggcaaccg tcacgctgtt gttaggaagt gtgccgctgt atgcgcaaac ggcggacgta
     4081 cagcaaaaac ttgccgaatt agagcggcag tcgggaggca gactgggtgt ggcattgatt
     4141 aacacagcag ataattcgca aatactttat cgtgctgatg agcgctttgc gatgtgcagc
     4201 accagtaaag tgatggccgc ggccgcggtg ctgaagaaaa gtgaaagcga accgaatctg
     4261 ttaaatcagc gagttgagat caaaaaatct gaccttgtta actataatcc gattgcggaa
     4321 aagcacgtca atgggacgat gtcactggct gagcttagcg cggccgcgct acagtacagc
     4381 gataacgtgg cgatgaataa gctgattgct cacgttggcg gcccggctag cgtcaccgcg
     4441 ttcgcccgac agctgggaga cgaaacgttc cgtctcgacc gtaccgagcc gacgttaaac
     4501 accgccattc cgggcgatcc gcgtgatacc acttcacctc gggcaatggc gcaaactctg
     4561 cggaatctga cgctgggtaa agcattgggc gacagccaac gggcgcagct ggtgacatgg
     4621 atgaaaggca ataccaccgg tgcagcgagc attcaggctg gactgcctgc ttcctgggtt
     4681 gtgggggata aaaccggcag cggtggctat ggcaccacca acgatatcgc ggtgatctgg
     4741 ccaaaagatc gtgcgccgct gattctggtc acttacttca cccagcctca acctaaggca
     4801 gaaagccgtc gcgatgtatt agcgtcggcg gctaaaatcg tcaccgacgg tttgtaatag
     4861 cggaaacgga atggggaaac tcattccgtt tttgtttatc gccttagacg gcaaaagtgc
     4921 tgtcgcccac ctgcgcttgc gcataccagg ccataagctc cgtggttcct ggttctcctt
     4981 ccgctggagc ccagtgcgca tagtcatcgg cagccacggg ttgatagcca ccgtgtttta
     5041 cttcaaaaat tatgccaccg gtatccagcg acagcacggc atgccaggtt cctgcggcca
     5101 tctccagcac cgtacaggtt tcccccaata tcgcccgatg ggtgacggta ccccgatcgt
     5161 caaaattcag caccacgaaa cgacccctta atggcaacag tagctcgaag gtgtgagggt
     5221 gtcggtgcgg gcgcacgtag gtcccaggtc atattccttc cggcgtccgg cattttaccg
     5281 ccagacagct cgggattcgt gatatcaccg ttcttgcaga atacggtcag agggaaaata
     5341 cccgccgtga gcatgcagcg ctgatacgtc agcactatca gtatcgtgaa tttgcctggc
     5401 cctggacatt tcgccttacc cgtcttttat atacccggag ctggataagc aacgaacgtc
     5461 ctggcctgct tttcgatctg gcgacagggt ggcttatgca acatcgtatt attctccccg
     5521 gagccactac gctgacccgg ttgatttcag aggtaaggga aaaggcgacg ttgcgcctgt
     5581 ggaacaaact ggcactgata ccgtcagccg aacagcgttc acagctggag atgctgctgg
     5641 ggccaactga ttgcagccgc ctgtctttac tggaatcact gaaaaagggc cctgtgacca
     5701 tcagtggtcc ggcgtttaat gaagcaattg aacgctggaa aactctgaac gattttggcc
     5761 tgcatgctga aaacctgagt acactcccgg ctgtgcgcct gaaaaatctc gcacgttatg
     5821 ctggtatgac ttcggtgttc aatattgcca ggatgtcacc gcagaaaagg atggcggttc
     5881 tggttgcctt tgtccttgca tgggaaacgc tggcgctgga tgatgcattg gacgttctgg
     5941 acgccatgct ggccgttatc atccgtgacg ccagaaagat tgggcagaaa aaacggctcc
     6001 gctcgctgaa ggatctggat aaatctgcat tggcgctcgc cagcgcatgt tcgtacctgc
     6061 tgaaagaaga aacaccggac gaatcgattc gtgctgaggt gttcagctac atcccaaggc
     6121 aaaagctggc tgaaatcatc acgcttgtcc gtgaaattgc ccggccctca gacgataatt
     6181 ttcatgaaga aatggtggag cagtacgggc gcgttcgtcg tttcctgccc catctgctga
     6241 ataccgttaa attttcatcc gcacctgccg gggttaccac tctgaatgcc tgtgactacc
     6301 tcagccggga gttcagctca cggcggcagt tttttgacga cgcaccaacg gaaattatca
     6361 gtcggtcatg gaaacggctg gtgattaaca aggaaaaaca tatcacccgc aggggataca
     6421 cgctctgctt tctcagtaaa ctgcaggata gtctgaggcg gagggatgtc tacgttaccg
     6481 gcagtaaccg gtggggagat cctcgtgcaa gattactaca gggtgctgac tggcaggcaa
     6541 accggattaa ggtttatcgt tctttggggc acccgacaga cccgcaggaa gcaataaaat
     6601 ctctgggtca tcagcttgat agtcgttaca gacaggttgc tgcacgtctt tgcgaaaatg
     6661 aggctgtcga actcgatgtt tctggcccga agccccggtt gacaatttct cccctcgcca
     6721 gtcttgatga gccggacagt ctgaaacgac tgagcaaaat gatcagtgat ctactccctc
     6781 cggtggattt aacggagttg ctgctcgaaa ttaacgccca taccggattt gctgatgagt
     6841 ttttccatgc tagtgaagcc agtgccagag ttgatgatct gcccgtcagc atcagcgccg
     6901 tgctgatggc tgaagcctgc aatatcggtc tggaaccact gatcagatca aatgttcctg
     6961 cactgacccg acaccggctg aactggacaa aagcgaacta tctgcgggct gaaactatca
     7021 ccagcgctaa tgccagactg gttgattttc aggcaacgct gccactggca cagatatggg
     7081 gtggaggaga agtggcatct gcagatggaa tgcgctttgt tacgccagtc agaacaatca
     7141 atgccggacc gaaccgcaaa tactttggta ataacagagg gatcacctgg tacaactttg
     7201 tgtccgatca gtattccggc tttcatggca tcgttatacc ggggacgctg agggactcta
     7261 tctttgtgct ggaaggtctt ctggaacagg agaccgggct gaatccaacc gaaattatga
     7321 ccgatacagc aggtgccagc gaacttgtct ttggcctttt ctggctgctg ggataccagt
     7381 tttctccacg cctggctgat gccggtgctt cggttttctg gcgaatggac catgatgccg
     7441 actatggcgt gctgaatgat attgccagag ggcaatcaga tccccgaaaa atagtcc
//
LOCUS       DACBVI010000192         7459 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.193, whole genome shotgun sequence.
ACCESSION   DACBVI010000192 DACBVI010000000
VERSION     DACBVI010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.193"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            156..1523
                     /locus_tag="IU325_20440"
     CDS             156..1523
                     /locus_tag="IU325_20440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAP5811367.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGR
                     LTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVVG
                     GAIVAHILSSHYESYNLHIAELTNENDQVVWKTSYVTIMIFMWLTLLSVNLYFNGLRC
                     DIWNGVTVIAFCAVLYNVSLLFMSRYSVSIWYISRTIEVVSKLTVMVIFMCHIFSALR
                     VTKDIAHRDSLTNIFNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVG
                     DQVIKTVVSIIGKSIRPDDLFARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQKNELNPKEIYQLADNALYEAKETGRNKVVVKEAEILIN
                     RKDDE"
     gene            complement(1628..1715)
                     /locus_tag="IU325_20445"
     tRNA            complement(1628..1715)
                     /locus_tag="IU325_20445"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1679..1681),aa:Ser,seq:gga)
     gene            1949..2887
                     /gene="ghrA"
                     /locus_tag="IU325_20450"
     CDS             1949..2887
                     /gene="ghrA"
                     /locus_tag="IU325_20450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HAP5811368.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWLHPRVAITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            2942..3679
                     /locus_tag="IU325_20455"
     CDS             2942..3679
                     /locus_tag="IU325_20455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAP5811369.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            3703..4257
                     /locus_tag="IU325_20460"
     CDS             3703..4257
                     /locus_tag="IU325_20460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAP5811370.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEGATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            4359..4850
                     /locus_tag="IU325_20465"
     CDS             4359..4850
                     /locus_tag="IU325_20465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HAP5811371.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLFS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAVRSTKTAHREQEIK
                     NKA"
     gene            complement(4914..5747)
                     /gene="csgG"
                     /locus_tag="IU325_20470"
     CDS             complement(4914..5747)
                     /gene="csgG"
                     /locus_tag="IU325_20470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HAP5811372.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(5774..6190)
                     /gene="csgF"
                     /locus_tag="IU325_20475"
     CDS             complement(5774..6190)
                     /gene="csgF"
                     /locus_tag="IU325_20475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HAP5811373.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDYGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(6215..6604)
                     /gene="csgE"
                     /locus_tag="IU325_20480"
     CDS             complement(6215..6604)
                     /gene="csgE"
                     /locus_tag="IU325_20480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HAP5811374.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(6609..7259)
                     /gene="csgD"
                     /locus_tag="IU325_20485"
     CDS             complement(6609..7259)
                     /gene="csgD"
                     /locus_tag="IU325_20485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HAP5811375.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
BASE COUNT         1937 a         1609 c         1799 g         2114 t
ORIGIN      
        1 gaagaatgta taaagcacta aggtaataat aattgcagtc tgcaactatc atcatcggta
       61 ttgtttgtgg gcggaatttc atattgacaa cagtacagtt cttatatcta ttaataatag
      121 aaagggatct acaacctaca gattggtgta gctttatgga aaaagactat ttgagaatta
      181 gtagtactgt attagtgagc ttattgtttg ggcttgcttt ggttttggtg aatagttggt
      241 ttaatcagcc aggcgttgag gaagttgttc cacgatcaac ctatctgatg gtaatgattg
      301 ctttattttt tatcgatact gttgcattta tttttatgca gttgtatttc atttatgacc
      361 gtaggcaatt ttcaaactgt atacttagtt tggcttttct gagttgtttg atttactttg
      421 ttaaaaccgt cattatcatt cagcaaatta ttgaggggcg tttgacaagc agtgttgttc
      481 aaaatgacat tgcaatctat tatttgtttc gtcagatgag tttgtgcata ttaatatttc
      541 tggcattggt gaataaagtt agtgaaaaca caaaacagcg caatttattt tccaaaaaaa
      601 tgactttgtg cataagtttg ttttttgttg ttgggggggc gatagttgct cacatacttt
      661 ctagtcatta tgagagctac aatttacaca ttgccgagtt aaccaatgaa aatgatcagg
      721 tggtttggaa aacatcatat gttaccataa tgattttcat gtggttaacc ttgctttcag
      781 ttaatctata ctttaatggt ttacgctgtg atatctggaa tggagtgacg gtgattgctt
      841 tttgtgccgt actgtacaat gtctctcttt tatttatgag caggtatagc gtgtccattt
      901 ggtatattag tcgcactatt gaagtagtca gtaagttaac cgttatggta atatttatgt
      961 gccatatatt cagtgcgcta cgagtaacaa aggacattgc acatcgcgat tccttaacca
     1021 atatatttaa cagaaattat ttttttaatg aactgacagt tcaatcagca tcggccaaaa
     1081 aaacgcctta ttgcgtcatg attatggata tcgaccactt caaaaaagtc aacgacacct
     1141 gggggcatcc ggttggcgat caggtgataa aaacagtggt gagtatcatt gggaaaagca
     1201 tacggccaga tgaccttttt gcgcgcgtcg gcggcgaaga gtttggcgtc ttgctgacgg
     1261 acatcgatac tgaacgtgcg aaagctttag cggaaaggat tcgggaaaat gttgagcgtt
     1321 taactggtga taatcctgaa tacgccatac cacaaaaagt gacgattagt attggcgctg
     1381 ttgtaacgca gaaaaatgag ttaaacccta aggagatcta tcaactggct gataacgcac
     1441 tttacgaggc gaaagagacc gggcgtaata aggtggtagt aaaagaggca gaaattctta
     1501 tcaaccgcaa ggatgatgaa tgagtctgtc gcaagttgtc gacctccgcc tggggcttct
     1561 catccccccg gtctatgcaa tatacgaaaa aaaagctcgt actttcgtac gagctcttct
     1621 ttaaatatgg cggtgagggg gggattcgaa cccccgatac gttgccgtat acacactttc
     1681 caggcgtgct ccttcagcca ctcggacacc tcaccaaatt gttgttcctg tcttgctgga
     1741 acgggcgcta atttagggaa atcatgacct gaggtcaaca aactttttta aaaaatcgcg
     1801 cgtttattca aacttcaatc aatatgcagt tttaataagc gaaatctgct ttttttgcca
     1861 ccgaccacgg atttgttatg ctggtggctt ttgtagatca taacgataag tgcgaataaa
     1921 tttcgcgcaa cgcttttcgg gagtcagtat ggatatcatc ttttatcacc caacgttcga
     1981 tacccaatgg tggattgagg cactgcgcaa agctattcct caggcaagag tcagagcatg
     2041 gaaaagcgga gataatgact ctgctgatta tgctttagtc tggcatcctc cagttgaaat
     2101 gctggcaggg cgcgatctta aagcggtgtt cgcactcggg gccggtgttg attctatttt
     2161 gagcaagcta caggcacacc ctgaaatgct gaagccttct gttccacttt ttcgcctgga
     2221 agataccggt atgggcgagc aaatgcagga atatgctgtc agtcaggtgc tgcattggtt
     2281 tcgacgtttt gacgattatc gcatccagca aaatagttcg cattggcaac cgcttcctga
     2341 atatcatcgg gaagatttta ccatcggcat tttgggcgca ggcgtactgg gcagtaaagt
     2401 tgcacagagt ctgcaaacct ggcgcttccc gctgcgttgc tggagtcgaa cccgtaaatc
     2461 gtggcctggc gtgcaaagtt ttgccggacg ggaagaactt tctgcatttc tgagccaatg
     2521 ccgggtattg attaatctgt taccgaatac ccctgaaacc gtcggcatta ttaatcaaca
     2581 attactcgaa aaattaccgg atggcgcgta tctcctcaac ctggcgcgtg gtgttcatgt
     2641 cgtggaagat gacctgctcg cggcgctgga tagcgggaaa gttaaaggcg caatgttgga
     2701 tgtttttaat cgcgaaccct taccgccgga aagtccgctc tggctacatc ctcgcgtggc
     2761 gataacacca catgtcgccg cgattacccg tcccgctgaa gctgtggagt acatttctcg
     2821 caccattgcc cagctcgaaa aaggggagag ggtctgcggg caagtcgacc gcgcacgcgg
     2881 ctactaataa agcatcaggt ttcctgctat ccttggcggg aattgaatac aggagagagt
     2941 tatgtatccc gtcgaccttc atatgcatac cgttgccagc acgcatgcat atagcacatt
     3001 gagtgattac attgcccagg ccaaacaaaa gggcattaaa ctttttgcga tcaccgatca
     3061 tggcccggat atggaagatg cgccgcatca ctggcacttc attaacatgc gtatctggcc
     3121 gcgagtggtt gatggggttg ggatcctgcg cggcatcgaa gctaacatta aaaatgttga
     3181 cggtgaaatc gactgcagtg gcaaaatgtt tgattcgctg gatctgatta ttgccggttt
     3241 tcatgagccg gtgtttgcgc cgcatgacaa agcgactaat acgcaagcga tgatcgccac
     3301 tatcgccagc ggcaatgtgc atataataag ccatcccgga aatccgaaat atgaaataga
     3361 tgttaaagcc gttgctgaag cagctgcgaa acatcaggtg gcgctggaaa tcaataattc
     3421 ctcattttta cactcacgta agggcagtga agacaactgt cgggcggtag ccgcagcggt
     3481 acgtgacgct ggaggttggg tggcattagg ctcggattct cacaccgcgt ttaccatggg
     3541 tgaatttgaa gagtgtctta aaatcctcga cgcggtagat tttccgccag agcgcatttt
     3601 gaatgtttct ccgcggcgct tactgaactt ccttgaatct cgcggtatgg caccgattgc
     3661 ggaatttgca gacctttaat tactagtaat ggaaatatat taatgaacga gttttctatc
     3721 ctctgtcgtg tgctgggttc gctctattac cgccaaccgc aagatccttt actggtgccg
     3781 ctgtttaccc tgattcgtga ggggaaactg gctgcgaact ggccactgga gcaggatgag
     3841 ttactgacgc gtttacagaa aagctgtgat atggcgcaag tctctgccga ttacaatgcg
     3901 ttgtttatcg gcgatgaatg tgctgtgccg ccatatcgta gcgcatgggt tgagggcgcg
     3961 acggaagcgg aagtgcgcgc ttttctttcc gaacggggga tgccattagc ggatacgcca
     4021 gccgatcaca tcggcacatt gctgctcgcg gcttcctggc tggaagatca gtcaacggaa
     4081 gatgagagcg aagcactgga aacactgttc agtgagtatc tgttaccctg gtgtggtgcg
     4141 ttccttggca aagtggaggc ccatgcaact acgcctttct ggcgcaccat ggcaccgcta
     4201 acccgcgatg ctattagtgc aatgtgggac gagctggaag aagattctga agagtaaatg
     4261 tgatctgcat cactcataac tgcaacagat aatttgttgt tgcataaaat gtgtgctcga
     4321 tctcattcat ggccgcgttt tctgctatca tgcgcggcat gaacatactt ctctctattg
     4381 caatcacaac aggcattctc tccggtatct ggggatgggt ggctgtttcc ctgggcttac
     4441 taagctgggc gggcttcctg ggctgtacgg cctactttgc ctgcccgcaa ggtgggctga
     4501 aagggctggc gatctccgct gcaaccctgc ttagtggcgt ggtgtgggcg atggtcatta
     4561 tttacggtag tgcactggca ccacatctgg aaattctcgg ttatgtcata accggtattg
     4621 tcgcgtttct gatgtgtatt caggccaaac agctgctgtt ttcatttgtt ccgggaacgt
     4681 ttataggcgc atgcgcgacg tttgccgggc agggtgactg gaaactggtg ttaccttcgc
     4741 tggcgctcgg gttgatattt ggttacgcaa tgaaaaacag cggcctttgg ctggcggtgc
     4801 gtagtacgaa gaccgctcac cgtgagcagg aaatcaaaaa taaagcgtga ggggcactca
     4861 cgctttcgct taaacagtaa aatgccggat gataattccg gcatttttat ctatcaggat
     4921 tccggtggaa ccgacatatg gcggtatttc accagaatgt cattctgccg ttctgctttg
     4981 ttctgcaaat cccaaagacc gcggtcgata ccatcattaa tcaggaaaat gacgcctgtt
     5041 tcgatagccg acatcaggca cagcataacg ggttcgttcg aggtgtaacc tacttcccct
     5101 tccagcaagc gctggtagtc aataaagcgg aagacccccg cctgaacttc ataggaaagt
     5161 atcgtcttac tggtgttcac cgaggagagg atctcaccgg tactcacatt gacgacgcgc
     5221 aggttcacgg caatctgatc gagctggtat tgcgtgtcgg caccgatccc aaaatatctt
     5281 gccccaacac cgcccgattt aacgttgctt tcataaccga taatcgaacc ttcaaccatg
     5341 atatttgccg ccgttaaaga ctgcagcggg attcggttat taatggcaac cgtaccgttt
     5401 tcttgtgccg cacgaataat cttgcgctcg ttaagcaggt tttgtaagcc ctggcgctcc
     5461 agcggtataa accagcgaga atctttcaat gccgtgacca gcattgccgt ggcgctttgc
     5521 ggaacagcag tggagaagtt acttgccggg tagggtttaa attgcccggt ttcgtcctga
     5581 atgttgtata ccgaaacaaa gatcttaccc gtcggcgctg gcagatgggt caaatctttg
     5641 tagctctgag cacgaggcat taatgtcggt ctggcggctt ctttaggcgg ggcggttaag
     5701 catccgctca gtaacatgac ggcaaccaaa agaaataagc gctgcatgat tattttcctt
     5761 atgaagctgg ggcttaaaaa tcggttgagt tattttgtaa acccgaaacc tggatggtcg
     5821 aggtttgtcc ggttttacga tctgtcacgt tcaactgcaa ttgaccatcg cggttagcaa
     5881 tatcaacaat ataatcgttg gtcaccatgc ggcccggttt accggtatta atattcgaca
     5941 gtagcccacc taaaatttgt gactggatgg cctgagtaaa gttatctaac gctgagggtg
     6001 tttcaatacc atagtcatcg ttatagctcg gatctttata cgagttttgt gcctgggcgc
     6061 tatttaataa aaaagcgcca ttatttgggt taccaccaaa gtttggatta cggaactgaa
     6121 aagtcatggt tccagcccaa cttaatggcg aaataagcat gagtagaact actgcatgtt
     6181 tgacacgcat tgcagcctcc gaacaatttt ttatttagaa ttcatcatgc gccaaatcgc
     6241 ccgtacttaa taacgcctga tttatctggc gacgatttag tgcttcttca gtttgaatca
     6301 gtgcaaagac gacagttttt tcgaagtctc ttttcaacgg gaataaaaaa gtctggaaaa
     6361 taacgtcctg attgaccgtt atagtgatcc agcttcccca tcgtgcactg ggcctttcat
     6421 taatcgttaa gttacccgta tagtcacttt cccatttatc actaaaggct cggtaaaaat
     6481 catggccaat agatgaaaca gtatggtcag ttaacaatcc cgggacttct acctcaacgg
     6541 cgtgaagatt ccctgcggcg aacagaaatt ctgccgccac aatccagcgt aaataacgtt
     6601 tcatggcttt atcgcctgag gttatcgttt gcccaggaaa ccgcttgtgt ccggtttttt
     6661 acggctatct tcttgaaaag attataaaga tgcgttttaa ccgtattttc gctgatgaac
     6721 aacgaacgag cgatctcgtt attagacgcg ccgatacgca gcttattcag gatctctttt
     6781 tcccgatgag taaggagggc tgattccgtg ctgttataac gatagttacc tgaatgcgta
     6841 atcaggtaac tggcaagctt ttgcgtaaag tagcattcgc cgcgcaggac gccttgcaac
     6901 ccattgacaa cacgttcttg atcctccatg gcataaaaaa cgccgttgat atgaggccag
     6961 ttttcaatgt cgcggtacgg gtaatcttca ggcgtattta gcaacaatat tttgatattg
     7021 ttgtttttcc tgctcaaagt atcctgccaa taatggataa gctttttatc cgcttccatc
     7081 atatccagaa gaataataga gcctgaagat atatcgtcca gagaacgttg aatattatgt
     7141 aattttcctg taattgccag cgattgttta aggtgctgca agagagctgt cgcctgcaaa
     7201 gaaggtttgg tgatcaacaa taatgtatga ccatgaatac tatggacttc attaaacatg
     7261 atgaaacccc gcttttttta ttgatcgcac acctgacagc tgcctctaaa atagaagcac
     7321 cagaagtact gacagatgtt gcactgctgt gtgtagtaat aaatcagccc taaatgggta
     7381 aaatataaaa ctaatggatt acatctgatt tcaatctagc cattacaaat cttaaatcaa
     7441 gtgttaaaca tgtaactaa
//
LOCUS       DACBVI010000193         7372 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.194, whole genome shotgun sequence.
ACCESSION   DACBVI010000193 DACBVI010000000
VERSION     DACBVI010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.194"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..904
                     /locus_tag="IU325_20490"
     CDS             <1..904
                     /locus_tag="IU325_20490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAP5811376.1"
                     /translation="STAAVLNMAATLPLVFDAELNSIRERLNIMKASPHNNNVWGATY
                     NTRNNVTTDAGAGFEQTLIGMTVGIDSRNDIPEGIAMLGAFMGYSHSHIGFDRGGHGS
                     VGSYSLGGYASWEHESGFYLDGIVKLNRFESNVAGKMSSGGAANGSYRSNGLGGHIET
                     GMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMESKSVDTRSIYRELGATLSYNMRLG
                     NGMEVEPWLKAAVRKEFVDDNRVKVNNDGNFVNDLSGRRGIYQAGIKASFSSSLSGHL
                     GVGYSHGAGVESPWNAVAGANWSF"
     gene            complement(1269..1535)
                     /gene="minE"
                     /locus_tag="IU325_20495"
     CDS             complement(1269..1535)
                     /gene="minE"
                     /locus_tag="IU325_20495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HAP5811377.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1539..2351)
                     /gene="minD"
                     /locus_tag="IU325_20500"
     CDS             complement(1539..2351)
                     /gene="minD"
                     /locus_tag="IU325_20500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HAP5811378.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2375..3070)
                     /gene="minC"
                     /locus_tag="IU325_20505"
     CDS             complement(2375..3070)
                     /gene="minC"
                     /locus_tag="IU325_20505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HAP5811379.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3590..3958
                     /locus_tag="IU325_20510"
     CDS             3590..3958
                     /locus_tag="IU325_20510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811380.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSVQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4061..4462)
                     /locus_tag="IU325_20515"
     CDS             complement(4061..4462)
                     /locus_tag="IU325_20515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor of g-type lysozyme"
                     /protein_id="HAP5811381.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4703..4996
                     /locus_tag="IU325_20520"
     CDS             4703..4996
                     /locus_tag="IU325_20520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811382.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     LLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5068..5727
                     /locus_tag="IU325_20525"
     CDS             5068..5727
                     /locus_tag="IU325_20525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HAP5811383.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5804..6265
                     /locus_tag="IU325_20530"
     CDS             5804..6265
                     /locus_tag="IU325_20530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415699.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HAP5811384.1"
                     /translation="MAEHLMSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDED
                     TDEIYFTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLA
                     EGKDLPAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(6472..>7372)
                     /gene="hlyE"
                     /locus_tag="IU325_20535"
     CDS             complement(6472..>7372)
                     /gene="hlyE"
                     /locus_tag="IU325_20535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HAP5811385.1"
                     /translation="VADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETIKELS
                     RFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFDEYNE
                     KKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEKSS
                     YFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQSFF
                     TTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAAKK
                     MINTCNEYQKRHGKKTLFEVPEV"
BASE COUNT         1889 a         1642 c         1795 g         2046 t
ORIGIN      
        1 ttctacggca gctgtactca atatggcagc aacattaccg ttggtatttg atgctgagct
       61 aaacagtatt cgcgagcggt tgaacataat gaaagcgagt ccacacaaca ataatgtctg
      121 gggggcgacg tataacaccc gtaataatgt caccaccgat gcgggtgccg ggtttgagca
      181 gacgctgatc ggaatgacag tggggatcga cagccgtaat gatattccag aaggaattgc
      241 catgctgggc gcttttatgg gttattccca ttcacatatc ggttttgatc gcggaggtca
      301 tggcagtgtg ggcagttatt ctctgggcgg ctatgccagt tgggaacatg aaagtggttt
      361 ctatctggac ggtatcgtga agctgaaccg ctttgaaagt aacgtagccg gtaaaatgag
      421 cagcggtgga gccgccaacg gcagttatcg cagcaacggg ctgggcggtc acattgaaac
      481 cgggatgcga tttaccgatg gtaactggaa cctgacgccg tatgcatcgt taacggggtt
      541 caccgctgat aaccccgaat atcatttatc caatggtatg gaatcgaaat cagtcgatac
      601 ccgcagtata tatcgtgaac tgggcgcaac gctgagttac aacatgcgtc tggggaacgg
      661 catggaagtt gagccgtggc tgaaggcggc tgtgcgcaaa gaatttgtcg atgataaccg
      721 ggtgaaagtg aataatgacg gtaattttgt caatgatttg tcgggcagac gtggaatata
      781 tcaggcaggt attaaagcct cattcagcag ttcgttaagc gggcatctcg gggtggggta
      841 tagccatggt gccggtgtgg aatccccgtg gaacgcggtg gctggtgcga actggtcgtt
      901 ctgaccatca acgattaaac tgcgcttcgg cgcagttttc gtttacaggg tgttaaaagg
      961 aaaaattctg cggcaaaaaa agcccgccag ttacggcggg aaacctcatc ctatgggaga
     1021 acaatgaata atgaaattgc ggggttatca tcacccagta taccaatact aacaataagg
     1081 ttatttactc agccaggtat aaacaatttg atctgtgtgg ggggaacggc attaaggtgt
     1141 aaagggggga agcaatgagt agcatcagca agaatactcg ccgctcttat caaacgtgga
     1201 tgagggggat gaaaaacgca aggcagagat gactctgcct tgaagagaaa tgcgctttta
     1261 cagcgggcct atttcagctc ttctgcttcc ggcaaggtca cgttcagctc aagaatagaa
     1321 atatcgccat ctttttgctc aagctgtacg gttaccatct caggatcaat ttgtacgtat
     1381 ttacaaatga cctcaagaat atctttacgc aactgcggca gataatgcgg ttctgcatcg
     1441 ctgcgacggc gttcagcaac aataatctgc agccgttctt ttgcaatgtt ggccgtgttt
     1501 ttcttccgcg agagaaagaa atcgagtaat gccataactt atcctccgaa caagcgtttg
     1561 aggaagcctt tcttctcttc ttcaatgaag cggaaaggac gttcttctcc caacagacgt
     1621 tctacggtat ctgcgtaggc tttacccgca tcggcgttaa tgtcgagaat gaccggttca
     1681 ccctggttag aggcgcgcaa tactgattga tcctctggga tcacgccgac gagtttgatg
     1741 cgcaggatct ccagcacatc ttccatgctc agcatgtcac ctctgcttac gcggcctggg
     1801 ttatagcgcg ttaacagcag gtgctcttta ataggctctt cgccattttc tgcgcggcgt
     1861 gatttcgacg ccagaatgcc taaaatacgg tcagagtcgc gtactgagga gacttccggg
     1921 ttggtggtaa taatggcttc gtctgcaaaa tagagtgcca ttagcgcacc ggtctcaatc
     1981 cctgccgggg agtcgcaaac aataaattca aagtccatcg ctttcagatc atcaagaact
     2041 ttggcgaccc cttcacgggt gagggcatct ttatcgcgag tttgcgatgc cggaagaata
     2101 tagagatttt cagtacgctt atctttaatt aacgcctgat ttagcgttgc atcgccctga
     2161 atgacgttga cgaaatcgta aacgacccga cgttcacaac ccataatcag gtcgagatta
     2221 cgcaggccga tatcaaaatc tatcacgaca gttttctttc ccttctgggc caaaccagtg
     2281 gcgatggccg cgctggaggt tgtcttacca acaccccctt tgcccgaagt aacaacaata
     2341 atgcgtgcca tagaaattcc ttgttaaaaa gggatcaatt taacggttga acggtcaaag
     2401 cgttttcgac taactgcagt cgcgccgctt tgccataaaa ttctgctggg atttgatcac
     2461 tcagccagta ttcacctgcg atggacacca attccgccat caggttcgta caaaatattt
     2521 gcgtttcccg gtcaccactt gcccctgcca gcgcacgacc gcgcatcatg ccatagacat
     2581 gaatgttccc atcggcaatc aattcggccc cagcgctaac gtggcttgta acaatcagat
     2641 cacattgtgg agcataaata cgctgaccgg aacgcaccgg ggtatctatt aaacgcgttt
     2701 ttgtgaccgg cgttgtattt tgcgctggag cctgcggtgc gggagctgga cgcggcgctt
     2761 tttcctttcc ttccgtcagg ataggcagcc ccattttttc aatttcggct ttaagttgcg
     2821 catctttgca gccacttacg ccaataaccc gcaaaccggt tgccgaaacc gccttatgca
     2881 tcgctgacca gtttaccggg tcttccagtg cactgacgtt gagtacaacg ggggcatgtt
     2941 ttaaaaatgc gggggcctga gcgattttgt cttccagcgc ctgatggata accttaggtt
     3001 ctgcctcatg cagatgaacc acagataaag tgaagctact gcctttaagc tcgattggcg
     3061 tgtttgacat cctggcctta ctcaattagc tattaatcat cgccagcgcg cgatgatgtt
     3121 ccgaagacta taaggcatgt tatagtctgg attatattga ggcaagtcac cctcccattt
     3181 attcagagta aaagtctatt ctgtgataaa tggcgctgat tcatagctta aaaaataccc
     3241 ttgtcaatca acccattgcc gtcgtacttt tgattcttct tatttacgct tcctttttcc
     3301 gcaccctaat cgtgacagca tctccaaacc tgtttaaata tccagtattg aggcaagtgt
     3361 attttttcac cacgccaggc tttcttaact ttccattttt tgcggcatca ttaaagaaca
     3421 gatacccgtt atcttaatat ccgccacaga attgctctat catagaaacc gcagaagacg
     3481 tctgatactt ctgtcaggtc atcgtaactc gtccaggtca gcggttttga ttacggattg
     3541 atgacgaaat gagcataagt gttgcacggt tggattatca ggatgtgcta tgaatatgat
     3601 gcgtattatt tatatcggat tgtcaggtgt ggggatgatg ttctcatcaa tggcgtctgg
     3661 acatgacgct ggcggacttc aatctcctgc gtgtggcgtt gtttgcgacc cgtatatttg
     3721 tgttaactca gatggcattt ccccagagtt aacaaggaaa tatctcggcg aaaaagccgc
     3781 tgaaaactta caatcagtac aaggctacga tcccagcgaa tttacattcg ctaacggtgt
     3841 attttgcgat gttaaagaaa aattatgtcg tgatgatcgt tattttggtg tggatggtaa
     3901 gcggagtgga aaaatcaatc aaaccaccac aaagatgtta tttatgtgtc gtgagtgatg
     3961 tcttgtttac attatgttct attcgtcatt aacctcttga gcgacagaga ggagtggggc
     4021 gttaacgttt atgacgggga gagtccccgt cggttgacat ttacttaatt tgaatatcga
     4081 cgttatattt tttagttttg tttttacggg cgtcgtttcg agtttgcagc actcgcagct
     4141 catatttgcc agaagcaggg agtgagtatt gtccgtgact atcgagttct ggggaatatc
     4201 tggataaatc aacagaatca tcgattcctg gcccgaagag gtaagtatca gcgccttcat
     4261 tagagatact cacatgcact ttctgacctt ttttggcata aaaggtatat gtatcgtaat
     4321 cgtatccctt tatttcgccg gagtactgag cgctgctatg gccttttctg aactcaacat
     4381 tgacattctt acccgcagca aagctcgtgg aggttagcag tgcaagcaat aatacagcct
     4441 tactgatgct cttgattttc ataggtagtc tcctgttcag aatgagtacg atgaactgtt
     4501 ataatataac aattcatcac aggagaattc cagtggcttt cctgtgttcg gttaaatccc
     4561 ggcgcggcat ttttgccggg ggatcactta ttaagggcgc atggactgaa caaaccgcag
     4621 aaatctacag ctggctggct tgtggcgcgg gtttcattga tgttagctta tgccaaaacc
     4681 tggcatctta aagagtaaaa gtatgttttg tgtgatttat cgaagcagca agcgtgacca
     4741 gacctattta tatgtcgaaa aaaaagacga tttttcgcgt gtacctgagg aactgatgaa
     4801 aggttttggt cagcctcagt tagcgatgat tctgctgctg gatgggcgga agaaactggt
     4861 caatgccgat attgaaaagg ttaaacaggc attaaccgag caaggttact atttgcagtt
     4921 accgccacca cccgaagatt tgctgaagca acatctttcc gtcatggggc agaaaacaga
     4981 cgacacgaac aaataaccga tatccggcgg tggcattatc tttgtcggcg cgggttttca
     5041 tatccacgat aaggtgaggg gaacgttatg tatcaacatc acaactggca aggtgcgctg
     5101 ctggattatc cggtgagtaa agtagtctgt gttggcagta actatgccaa acatattaaa
     5161 gagatgggca gcgcagtgcc cgaagagcca gtgctgttta ttaaaccaga aacggcactg
     5221 tgcgatctgc ggcagccatt ggcgatccca tccgatttcg gttcagttca tcatgaagtc
     5281 gaactggcgg tattgattgg cgcgacgctg cgtcaggcta cggaagagca tgtccgtaaa
     5341 gccattgccg gttacggcgt ggcgctcgat ttgacattgc gtgatgttca gggaaaaatg
     5401 aagaaagccg ggcagccgtg ggaaaaggcc aaagcgtttg ataactcttg tccgctttcc
     5461 gggtttattc ccgcggcgga attcaccggc gatccgcaaa atacaacgct gggcctgagc
     5521 gtaaacggcg aacaacgcca gcaaggtacg actgcggaca tgatccataa aatcgttccg
     5581 ctgatcgctt atatgagcaa gttctttacc ctcaaggccg gtgacgttgt gctgacaggc
     5641 acgcctgatg gcgtcggccc gttgcaaagt ggtgatgagc tgacagtcac tttcgatggg
     5701 cattctttga caactcgcgt tttgtaatac tttttgccgc ctgaaagcgg cggcaaaact
     5761 tgcatcgctg tgccagactg gttataaggt gcgttttaac gtaatggcgg aacacctgat
     5821 gagcgatgta cctttctggc aaagtaaaac cctggacgaa atgagcgatg cggaatggga
     5881 gtcgttgtgt gatggttgcg gtcagtgttg cctgcataaa ctgatggatg aagacaccga
     5941 cgaaatctac ttcactaacg tcgcctgtcg ccagctcaat attaaaacct gtcaatgtcg
     6001 gaactacgaa cgtcgtttcg agtttgaacc tgactgcatt aaattaaccc gtgaaaatct
     6061 gccaacattc gaatggctgc caatgacctg cgcttatcgc ttgctggcgg aaggtaaaga
     6121 tttacctgcg tggcatccgc tgcttactgg ttcgaaagcg gcaatgcatg gcgaacgtat
     6181 ctctgtgcgt catatcgcag tgaaagaatc agaagtgatt gactggcagg atcatatctt
     6241 aaataaacct gactgggcgc agtgataaat ttaatagatg ttgatggggg ctttcttaat
     6301 atacgtctgg atactcgcca tttcgtagcc ctgtttttga aagcgctctg tcataatgag
     6361 agttacccga taccttatta tcaacaggta actctcaatc tttaaaaaac ggctgtgttt
     6421 ttaaatgtga taaaccttat acctggagta catggagaga ataatcgctt atcagacttc
     6481 aggtacctca aagagtgtct ttttaccgtg tcttttctga tactcattac aggtgttaat
     6541 catttttttg gccgcttctt ttagcaaaga aagcattaaa tcatcataat caacgtagaa
     6601 tctggttgtt tcagtttccg tttttatctc cccgatggcg gctatttcgg tggttaattt
     6661 caatttggcg gcatcgatat ctttattcgc ttgtttaacc gtgttagaca gggtggtaaa
     6721 gaaactctgc acagatttta acttgttctt caattctggg atcagtttcc cttcaactac
     6781 gcccgcagca atagaatagg aaatgattaa tccaaatgga ccggcgacga caccggctgc
     6841 ggcaccggca tacgcttcct tcctgatttt atctacctgt gactggaaat agctgctttt
     6901 ttctgaaaaa tcattggtta actggctatc taacgccagc agtttcccgg aagcgttgtt
     6961 gaaactttgt gagcttacca gcagggattt ttgcgcttca ttcagcttcg tgatgccgtc
     7021 atccagtacc ttaatgagaa tgtctttctg ggcggatgct ttcttctcat tgtactcatc
     7081 aaatagcaaa atatacgctg cgagcaattg cgtcgcaaca ccacaccatt catacaccgt
     7141 ttgggttgct tcaaaatact tatcctggct atccataagt aaggttttaa tatcgccaac
     7201 taaaacggag gctgcctgtg aatactcctg tttaaagcga cttaactctt ttatggtttc
     7261 atcgaaggtc tgccagggga tgacctgatc gagatattta ttataaaggt ctaatgctcc
     7321 atctgcggtt tcgattgcgt ttttaactac ctctaccgtt ttatctgcaa cg
//
LOCUS       DACBVI010000194         7304 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.195, whole genome shotgun sequence.
ACCESSION   DACBVI010000194 DACBVI010000000
VERSION     DACBVI010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.195"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..524)
                     /locus_tag="IU325_20540"
     CDS             complement(<1..524)
                     /locus_tag="IU325_20540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP5811386.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHL"
     gene            1002..1790
                     /gene="fabI"
                     /locus_tag="IU325_20545"
     CDS             1002..1790
                     /gene="fabI"
                     /locus_tag="IU325_20545"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP5811387.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELAKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            1934..3061
                     /locus_tag="IU325_20550"
     CDS             1934..3061
                     /locus_tag="IU325_20550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HAP5811388.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDPLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTTELCQLAETLSLHPISLSLLEKLLNSTRVNTQPDNQLAALICARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            3129..5063
                     /gene="rnb"
                     /locus_tag="IU325_20555"
     CDS             3129..5063
                     /gene="rnb"
                     /locus_tag="IU325_20555"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HAP5811389.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DGKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDLVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHIDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETAYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(5140..5243)
                     /locus_tag="IU325_20560"
                     /pseudo
     CDS             complement(5140..5243)
                     /locus_tag="IU325_20560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5297..7282
                     /gene="pdeR"
                     /locus_tag="IU325_20565"
     CDS             5297..7282
                     /gene="pdeR"
                     /locus_tag="IU325_20565"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HAP5811390.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPERTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGISLSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESKKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
BASE COUNT         1790 a         1853 c         1798 g         1863 t
ORIGIN      
        1 tgcagatgtt gtggatcgct tttttgcggt gttaaacgac gataaaccgt accttccatc
       61 atggtagcaa acacttcaac gcagcaacga atgtgctcgt cgctaagatg gggatgcatt
      121 cttttcatat gtgcacaacc gttagcgaac attctggcgt cagcttcttc aagcattgcc
      181 ataacccgcg ggttgcgtgt ggcttctgca gccacttcca gcatcagcgc gtcgtcgtcc
      241 tcatttaagg atttacgcaa ggcaaggact tccgggaagt gatcggtacg cgcgtcaatg
      301 tccatttggg caatacgaaa gtcgatgata cggcgaacca tctcttcaat aatggcatct
      361 ttgttggcga agtaacgata tatctgaccc acgctaagct gagcttcgct ggcaatttgc
      421 gacatactgg cggcatgaaa cccgcagcga cgaaaacacc gacgggcggc gttaatgatt
      481 tcatcctgac gctgtttgtg gcgtatctcc agtttgctgg tcatttctgt ctcctggcct
      541 atcacttggt gagaatgatc gttctcattt ttgctgatta tacgtttctc caaagtaatg
      601 gaaagtattt ttatcttatt catggtgcat attctgataa tgaagagatt gtggagattc
      661 tgtgaagata gttgacaata ttaagttggg gtcaattcat cagaaaggct gctacttgtt
      721 gtcgccagtt ctgttttcat catcaaactt atcggagcta atacgaatag tttcagagtc
      781 agattctata agatattccc ccaacctacc ccttgcaggc gtcaagagta gataacgcaa
      841 aatgctctgt acctcagctt ctctccggta cagaaagcgc aactatagcc acccacagcc
      901 aggttgatta taataaccgt ttatctgttc gtactgttta ctaaaacgac gaatcgcctg
      961 attttcaggc acaacaagca tcaacaataa ggattaaagc tatgggtttt ctttccggta
     1021 agcgcattct ggtaaccggt gttgccagca aactgtccat cgcctacggt atcgctcagg
     1081 cgatgcaccg cgaaggagct gaactggcat tcacctacca gaacgacaaa ctgaaaggcc
     1141 gcgtagaaga atttgccgct caattgggtt ctgacatcgt tctgcagtgc gatgttgcag
     1201 aagatgccag catcgacacc atgttcgctg aactggcgaa agtttggccg aaatttgacg
     1261 gtttcgtaca ctctattggt tttgcacctg gcgatcaact ggatggtgac tatgttaatg
     1321 ccgttacccg tgaaggcttc aaaattgccc acgacatcag ctcctacagc ttcgttgcaa
     1381 tggcaaaagc ttgccgctcc atgctgaatc cgggttctgc cctgctgacc ctttcctacc
     1441 ttggcgctga gcgcgctatc ccgaactaca acgttatggg tctggcaaaa gcgtctctgg
     1501 aagcaaacgt gcgctatatg gcgaacgcga tgggtccgga aggtgtgcgt gttaacgcca
     1561 tctctgcggg tccgatccgt actctggcgg cttccggtat caaagacttc cgcaaaatgc
     1621 tggctcattg cgaagccgtt accccgattc gccgtaccgt taccattgaa gatgtgggta
     1681 actctgcggc attcctgtgc tccgatctct ctgccggtat ctccggtgaa gtggtccacg
     1741 ttgacggcgg tttcagcatc gctgcaatga acgaactcga actgaaataa tcgttctgtt
     1801 ggtcaagacg ggcggcgttc tgccgcccgt tatctctgtt atacctttct gatatttgtt
     1861 atcgccgatc cgtctttctc cccttcccgc cttgcgtcag gataacgatt tcctttacga
     1921 ccaaggagcg cccatggaac aacgccacat caccggcaaa agccactggt atcatgaaac
     1981 gcaatccagt actgcggaat atgacgttct gcctctggtc ccggaagccg caaaggtcag
     2041 cgatcccttt ctgctcgacg tgatccttga tgaagaaacg ctggccccct tcctttcatg
     2101 gctggtccct gcgcgcgttc ttgcagtgga gttgttccct gacccgctta ccgtgacccg
     2161 ttcacagacc ttcaccgctt atgaacgctt gtcgacggcc ctgacggttg ctcaggtttg
     2221 cggcgtccaa cggttatgta actactattc ggcgcgactt acgccgctcc ccgggcctga
     2281 ttcctccagg gaaagtaatc atcggttggc acaaatcacg caatatgccc gccaactggc
     2341 tagctcgcct tctattatcg acaaccgatc gcgccagcat ctgaatgacg tcggtcttac
     2401 tgtctgggac tgtgtgatca ttaatcaaat cattggtttt attggctttc aggcgcggac
     2461 cattgcgaca tttcaggctt atctcgggca tccggtacgc tggttacccg gtctggagat
     2521 acaaaactat gccgacgcgt cactgtttgc tgatgaatca atacgctggc gaagcagcta
     2581 tgaggtggaa aaactacctg aagagtacac aaaaagttca actacagaac tttgccaact
     2641 ggccgaaaca ctctctctcc accctatttc actttccctt ctcgaaaagt tgttaaacag
     2701 cacacgggtt aatacacagc cggataatca gcttgcggcg ttgatatgcg cgcggataaa
     2761 tggcagtcct gcttgttttg ccgcctgtat ggattcatca aatgaatata aaaaaatcag
     2821 cccccttctg cgcaagggcg aaaatgaaat taaccaatgg gctgaccgtc attctgttga
     2881 gcgcgctacc gttcaggcga tacaatggct gacccgagca cccgatcgct ttagcgccgc
     2941 ccagttcagc cctttactcg aacacgaaaa atcatcaacg cagattatta atctgctggt
     3001 atggagcggg ctgtgtggct ggataaatcg cttaaaaatc gcgttgggtg agacatatta
     3061 accttgccgc gtcagacaga ttcgcgtaaa actgtcagcc gctctaatgg ccaccaaaat
     3121 agacaattat gtttcaggac aacccgctgc tagcgcagct taaacagcaa ctgcattccc
     3181 agacgccacg cgctgaaggg gtggtaaaag ccacagaaaa aggctttggc ttcctggaag
     3241 tcgacgcgca aaaaagttat ttcattccgc cgccgcagat gaaaaaagtc atgcatggcg
     3301 accgaattat cgcggttatc cacagtgaaa aagaacgtga atccgcagag ccagaagaac
     3361 tggttgaacc gttcctgact cgtttcgtgg gtaaagttca gggcaaaaat gaccgtctgg
     3421 ccatcgttcc tgatcatcca ctcttaaaag acgccattcc ttgccgcgca gcccgtggcc
     3481 tgaaccacga gtttaaagaa ggcgactggg cagttgccga aatgcgccgc catccgctga
     3541 aaggcgatcg ttctttctat gctgaactga cacaatacat cacttttggt gacgaccact
     3601 ttgtaccgtg gtgggttacc cttgcgcgcc ataatctgga aaaagaagca ccagacggcg
     3661 tcgctaccga aatgctcgat gaaggtctgg ttcgtgaaga tctgaccgcg ctggattttg
     3721 tcaccatcga cagtgccagc acagaagata tggatgacgc ccttttcgct aaggcgttgc
     3781 cggatggcaa acttcagctg attgtggcga ttgccgatcc aaccgcgtgg attgctgaag
     3841 gcagcaagct ggacaaagcc gcgaaaattc gcgcattcac caactatctg cctggcttca
     3901 acatccctat gctgcctcgc gagctttctg acgatctctg ctcactgcgc gccaatgaag
     3961 tccgcccggt actggcatgc cgcatgacgc tctccgctga tggcaccatt gaagataata
     4021 tcgaattctt tgctgccacc atcgaatcca aagcgaagct ggtgtatgac ctggtttctg
     4081 actggctgga aaataccggt gactggaagc ctgaaagtga agcaattgcc gaacaagtcc
     4141 gtttgttagc gcaaatttgc caacgccgcg gcgagtggcg tcataaccac gcactggtat
     4201 ttaaagatcg cccggattac cgctttattc tcggtgaaaa aggtgaagtg ctggatatcg
     4261 tcgccgagcc tcgtcgcatt gccaatcgta tcgtcgaaga agcgatgatt gccgctaaca
     4321 tttgtgcggc ccgcgtactg cgcgataagc tcggttttgg catctataac gtgcatatgg
     4381 gctttgatcc ggcgaatgcc gacgcgctgg cagcgttgct gaaaacccac ggtctgcata
     4441 tcgatgccga agaagtgctc acgctggacg gtttctgcaa actgcgtcgt gaactggatg
     4501 cgcaaccaac tggtttcctc gacagccgca ttcgtcgctt ccagtcattt gctgaaatta
     4561 gcactgaacc cggtcctcac tttggcctcg gtctggaggc atacgccacc tggacttcgc
     4621 cgatccgtaa atatggcgac atgatcaacc accgtctgct gaaagcggtt atcaaaggcg
     4681 aaactgcgac gcgtccgcag gatgaaatca ctgtccaaat ggccgagcgt cgccgtctca
     4741 accgaatggc agaacgtgat gttggcgact ggttatacgc acgcttcctg aaagacaaag
     4801 ccgggaccga cacccgtttc gcggcggaaa ttgtcgatat cagccgtggc ggcatgcgtg
     4861 tgcgtttggt tgataacggc gctattgcct ttattccggc acctttctta cacgctgtgc
     4921 gcgatgaact ggtttgcagc caggaaaacg gcactgtaca aattaaaggt gaaacggctt
     4981 acaaagtgac tgacgttatt gacgtcacca ttgccgaagt ccgcatggaa acccgcagca
     5041 ttattgcgcg cccggtcgcg taatctcctt tcacggccca ttcctcatga atgggccgtt
     5101 tatttccccg ctccaccttc gtcatatccc ggcagatttt taactgactt tcgtttgaaa
     5161 actggcgttt ttccaaaact ggattacaaa taaatatata aaaaaacaat gcgtttagcc
     5221 gccgttaatt tcatgtaagt catgttgttc cttttattta accgcggact ccgctgttaa
     5281 ccggaggata tgcatcatga aaaccgttag ggagtccaca acgttgtaca actttctcgg
     5341 atcgcacaat ccatactggc ggttaacgga aagcagcgat gttttgcgct tttctaccac
     5401 cgaaaccaca gaacctgaac gtacgttgca gttatctgcc gaacaggctg ctcgcatcag
     5461 ggaaatgaca gtcatcactt ccagtctgat gatgagtctg accgtcgatg aaagcgatct
     5521 ttctgtgcat ctggtaggac gaaaaatcaa taaacgggaa tgggctggca acgcgtcggc
     5581 atggcatgac acaccggcgg ttgctcgtga tttatcacac gggctttcct ttgctgagca
     5641 ggtagtttct gaagcacatt ccgcaatagt gattctcgac agtcggggga atatccagcg
     5701 cttcaatcgg ttatgtgaag attacacagg gttgaaagaa cacgacgtca ttgggcaaag
     5761 cgtgtttaaa ctgtttatga gccgtcgtga agctgcggca tccaggcgca ataaccgtgt
     5821 attttttcga agcggcaatg catatgaagt cgaactgtgg ataccaacac gtaaaggcca
     5881 gcggctgttt ctgtttcgca ataaatttgt ccacagcggc agtggcaaaa acgagatttt
     5941 tttaatctgt tccggcaccg acattaccga agagcgccgc gctcaggagc gactgcgcat
     6001 tctggcaaat accgacagta tcaccggact gccgaatcgt aacgcaatgc aggagttaat
     6061 cgatcacgct attaatcagg cagataacaa taaagttggg gttgtgtatc ttgatttgga
     6121 taattttaaa aaggtcaacg acgcctatgg gcatttgttt ggtgaccagc tattacgcga
     6181 cgtgtcattg gccattttaa gctgtctgga gcatgaccag gttttggcgc gtccaggtgg
     6241 cgatgagttt ctggtactgg catccaatac ctcacaaagc gcgctggaag caatggcatc
     6301 acgaattttg actcgcttac gactcccctt ccgcattggt ttaattgaag tttataccag
     6361 ctgttcagtg ggtatttcac tctctcccga acatggttcg gacagcgcgg ctattattcg
     6421 tcacgccgac acagcaatgt acacagcgaa ggaaggcgga cgaggacaat tttgcgtttt
     6481 tactccagaa atgaatcagc gggtatttga atatctctgg ctggatacca acttgcgtaa
     6541 agcactggaa aacgatcagt tggttattca ctatcaaccg aaaatcacct ggcgtggcga
     6601 agtgcgcagt ctggaagctc tagtacgttg gcagtcacct gaacgtgggt tgattccacc
     6661 gttggacttc atttcctacg ccgaagagtc agggctcatt gtgcctttag gccgttgggt
     6721 gattctcgat gtcgtacgcc aggtggcaaa gtggcgggat aaaggtatta acctgcgagt
     6781 ggcggtaaac atttctgcac gtcagctcgc cgatcaaacc attttcaccg ccctgaaaca
     6841 ggttctccag gaactcaatt ttgaatactg ccctattgat gttgaactga cggagagctg
     6901 cctgattgag aatgatgaac tggcactttc tgttattcaa caatttagcc aactaggtgc
     6961 gcaagtgcat ctggacgatt ttggtaccgg ctactcttca ctttcgcaac tggcgcgctt
     7021 tccgatcgat gcgattaaac ttgaccaggt ttttgttcga gatattcaca aacaacctgt
     7081 atcgcagtcg ctagtccggg cgatcgtcgc tgtggcccag gcattgaatc ttcaggtgat
     7141 cgctgaaggt gtggagagta aaaaggaaga tgctttttta accaagaacg ggatcaatga
     7201 gcggcaagga tttttgtttg ccaaaccgat gcccgccgtc gccttcgaac gttggtataa
     7261 acgctatctg aagcgcgcat aagtagatga atatatatac ttag
//
LOCUS       DACBVI010000195         7279 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.196, whole genome shotgun sequence.
ACCESSION   DACBVI010000195 DACBVI010000000
VERSION     DACBVI010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.196"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(134..769)
                     /gene="msrQ"
                     /locus_tag="IU325_20570"
     CDS             complement(134..769)
                     /gene="msrQ"
                     /locus_tag="IU325_20570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HAP5811391.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(770..1774)
                     /gene="msrP"
                     /locus_tag="IU325_20575"
     CDS             complement(770..1774)
                     /gene="msrP"
                     /locus_tag="IU325_20575"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HAP5811392.1"
                     /translation="MKKNQFLKESDVTAESIFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(1883..2296)
                     /gene="hiuH"
                     /locus_tag="IU325_20580"
     CDS             complement(1883..2296)
                     /gene="hiuH"
                     /locus_tag="IU325_20580"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HAP5811393.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2429..3100
                     /gene="hprR"
                     /locus_tag="IU325_20585"
     CDS             2429..3100
                     /gene="hprR"
                     /locus_tag="IU325_20585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HAP5811394.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3100..4458
                     /locus_tag="IU325_20590"
     CDS             3100..4458
                     /locus_tag="IU325_20590"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HAP5811395.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDVLIIHGDSINKIVNRTNVSDDMLN
                     NIPASETISAADIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSHIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(4565..5416)
                     /gene="hchA"
                     /locus_tag="IU325_20595"
     CDS             complement(4565..5416)
                     /gene="hchA"
                     /locus_tag="IU325_20595"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HAP5811396.1"
                     /translation="MTVQTSKSPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            5672..5884
                     /locus_tag="IU325_20600"
     CDS             5672..5884
                     /locus_tag="IU325_20600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811397.1"
                     /translation="MLWNIVVLMNIYIPDWWRKSFFHALFLQIFLYGKRVCYGLIHKE
                     MHLGVGLKSRWLMLLCQEGQGKGVYR"
     gene            complement(6008..7201)
                     /gene="ompC"
                     /locus_tag="IU325_20605"
     CDS             complement(6008..7201)
                     /gene="ompC"
                     /locus_tag="IU325_20605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAP5811398.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEVYNKDGNKLDFYGKVAGLHYF
                     SDNSGADGDMSYARIGFKGETQIADQFTGYGQWEYNLQANGTEGDGDNSATRLAFAGL
                     GFGQNGTFDYGRNYGVVYDIEAWTDMLPEFGGDTYAGADNFMNGRTNGVATYRNNGFF
                     GQVDGLNFALQYQGNDKSGGPFGQEGSGSGDGRSLSKENGDGFGMSTSYDFDFGLSLG
                     AAYSNSDRSDRQVGVGLNDRNHSNRNAGGETAEAWTVGAKYDANNVYLAAMYAETRNM
                     TPYGGGEFDNGESRSAIANKTQNFEVVAQYQFDFGLRPSIAYLQSKGKDLGGWAHDGN
                     GDPRYTNKDLVKYVEVGATSYFNKNMSTYVDYKINLLDNDDDFYEANGIATDDIVAVG
                     LVYQF"
BASE COUNT         1991 a         1580 c         1720 g         1988 t
ORIGIN      
        1 aataaaatga attatgttga taatttgagg ttgatgacaa acgaaacata acctgatgtg
       61 atctgcgtag gccggatagg gcgctcgcgc cgcatccggc agggataacg cgtactttgt
      121 cggtaatatg aagttataca gataatttgt tgtgaacctg cttgcgtaac cggttaaaaa
      181 gggaacgcaa cttcttatac cgtaaggcta aaagcagtac agccagccca gcgtagatga
      241 gcggctgcgg tgaaataatc ttcactgacc acagataatg tattggggcc aggatcgcga
      301 caagatagac gaaattatgc aactgttgcc agtgcttgcc gagttttcgc tgcatcgcct
      361 gggttgaagt aaacgctaaa gcaagcaaaa taacccagct gataattcct aacgttaaat
      421 aaggccgggt aattaactcc ttgcctaata acgccagatt attcacgccc agctccagca
      481 atgcgtaact ggttaaatgc agtgtcgccc aggcaaagca ccataagcct aacaggcggc
      541 gagtgcgtat caataacggc tgttttgcgt agcgtgccag aggtgtgatt aacaaggtcg
      601 ccagcaaaaa tttcagtgca gtgcgaccgg taaaatgctg aatatctttc accggatcgg
      661 cacccagtcc accgtgattg atcgcccaga ccagccagag aaacggcaac aatccggcaa
      721 gatgcaggct aactttcagc catgtcacct gttttgctgt cagacgcatt cagaaattct
      781 cccgcaaatc caggccacga tacagcgatg ccacctggtc ggcgtaacca ttaaacagta
      841 gcgttggctg gcgctgaaca tcgaggatgc cgcctgaacc aataaatcgt tcggtagcct
      901 gtgaccagcg cgggtgatca acatgcggat taacgttggc gtaaaaaccg tattcgtcag
      961 gcgctgccag attccaggtg gttggcggac gctcgcgggt cagcttaata ctgactatcg
     1021 atttaatccc tttaaagcca tatttccacg gcacaatcag tcgtaccggc gcgccatttt
     1081 gtggcggtaa cgccttgcca taaacaccca cggtcatcag tgtgagcgga tgcattgctt
     1141 cgtcgagacg caatccttcg acataaggat atttcagccc gccgccgata aagcggtcct
     1201 gctggccagg catctgttcc ggagcataaa ttgtttcgaa agcgacatac ttcgcattgc
     1261 tggtgggttc ggcaagcgcc agcaatttgt gcagcggaaa accaatccac ggcaccacca
     1321 tcgaccatgc ctccacgcag cgcatacgat aaatacgctc ttccagcggg aagcgacggg
     1381 ttaaatcatc gtgatcgagg gtcaatggtt ttgccacttc gccgctgatt ttcagtgtcc
     1441 atggatcggt tttcaggcta ccagcattag cggcgggatc ggctttatcc agcccgaatt
     1501 catagaagtt gttataaccg gagactttat ctactggcgt cagtggcagg ttattttgcc
     1561 aggcggcagg cttgctgaac tccagcggtt ttccggcggg ggcgggcggg cgatcgttcc
     1621 ctttaaacca gctaagcaga tcggcatgcg cagcgtgagg caaagaaagt gcagctgcgc
     1681 tgatgcccag tgcttttaac acctgtcgac gcttcataaa gaatatcgac tcggccgtaa
     1741 catctgattc ttttaaaaat tgattctttt tcataagcca tcacactttt ttgtcgttag
     1801 ccagcaagag ccgtttactg ttaatctgtc agggagtgtc ctccctaaca gtagcagaga
     1861 tctgctttta catatttatg cattaactgc cacgataggt tgaataccca tattggctaa
     1921 gtaataaagg cacatgataa tgctcgttca ctttattaat atgaaattca acggggatct
     1981 cagggaagaa actttcaaga ttttgtttct tgaaatagtc cccggtttta aatacgacac
     2041 ggtaatcgcc cgtagttgca gtttgctcgg gccacagtgc cttaattcgt ccatccttat
     2101 ctgttttggc ggtattaagt tgtaaccagc cgttgtccgc tttcttttca agagtgactg
     2161 tcacgtcggc agcgggtttt ccggtttgct ggttcaaaat gtgcacgcta agaatgtttt
     2221 gttgtgcggc ataaaccaaa gaaggtaatg aaaatgctgc cgttactacg gagagtacta
     2281 aataacgctt taacatgttt atatccttgt catgtgaatg agttttgagg atagcgaaaa
     2341 agcggccagg gtaaccttac agcagcatta caattttgta atgaaccagg ctgtttctat
     2401 aacatatgat ttatggcata ttattttcat gaagatttta cttattgaag ataatcaaag
     2461 gacccaggag tgggtaacgc aggggctttc cgaagcgggt tatgtcattg atgccgtttc
     2521 tgatggcaga gatgggcttt atcttgcgct gaaggatgat tatgcattga tcattctgga
     2581 tattatgctt ccgggtatgg atggctggca gatcttacaa acgttaagaa cagcaaagca
     2641 aacccctgtt atttgcctta ctgcaaggga ttctgtcgat gacagagtca gagggctgga
     2701 cagtggagca aatgattatc tggtaaaacc tttttcattt tctgagttgc tggcaagggt
     2761 tcgggcacaa ttaaggcaac atcacgcttt gaattcaaca ttagaaatca gtggcttaag
     2821 aatggactct gtaagtcaaa gtgtgagcag ggacaatatc agtattacac tgacgcgcaa
     2881 ggagtttcag ttactttggc tactggcctc cagagctggc gaaattatac ccagaacggt
     2941 tattgcgagt gaaatttggg gaatcaactt tgatagtgat accaatacgg tggatgtcgc
     3001 cattcgcagg ctccgcgcaa aagttgatga tccttttcct gaaaagctaa tcgccacaat
     3061 ccgggggatg ggctattcat tcgtagcggt aaaaaaataa tgaaaagatt atctataacc
     3121 gtccgtttaa ccttgctttt tatattgctg ctgtctgttg ctggcgctgg aattgtctgg
     3181 actctctata atggcctggc aagtgagttg aaatggcgcg atgatacaac actcattaac
     3241 cggacagcac agatcaagca gttgttaatt gatggggtaa atccagatac gttacctgta
     3301 tactttaacc ggatgatgga tgttagtcag gatgtcttga tcattcatgg tgatagcatc
     3361 aataaaattg ttaaccggac aaatgtcagt gatgacatgt taaataacat acctgctagt
     3421 gagacaatca gcgcagctga catttacaga agcattatta atgatactga gattgatgct
     3481 ttacgaatta atattgatga agtttcgcca tcattaacgg ttactgtggc taaattggct
     3541 tcagccagac ataacatgct tgaacagtat aaaattaata gcattataat ttgcattgtc
     3601 gccattgtac tttgctcagt attaagtccg ctgttaatca gaacgggatt acgagagatc
     3661 aaaaagttga gtggtgtaac ggaagcgctg aattataacg atagccggga gcctgttgag
     3721 gttagcgcat taccgagaga actaaaacct cttgggcagg cgttgaataa aatgcatcaa
     3781 gccttagtca aagattttga acgcctaagt caatttgctg acgatctcgc tcatgaactt
     3841 agaacgccca ttaatgcatt actgggtcag aatcaggtta cgctcagtca aaccagaagt
     3901 atcgctgaat atcaaaaaac aattgccggt aacattgaag agctggaaaa tatttcgcgg
     3961 ttaacagaga acatactgtt tcttgcccgg gcagataaaa acaatgtttt ggtgaaactg
     4021 gactcgcttt ctctcaataa ggaagtcgaa aatctgttgg attatcttga atacctttca
     4081 gacgagaaag agatttgctt taaggtcgag tgcaatcagc aaatctttgc ggataaaatt
     4141 ttactgcaac gaatgttatc gaatcttatt gttaatgcca ttagatattc accagaaaaa
     4201 tcgcatattc atataaccag ttttcttgat accaacggct atcttaatat tgatgtcgcc
     4261 agtcctggaa cgaaaattca tgagcctgaa aaactcttcc gtagattttg gcggggagat
     4321 aattcgcgtc attccgtagg tcagggacta ggcctttctt tagtcaaagc gattgccgaa
     4381 ttacatgggg gaagtgctac gtatcactat ctcaataagc ataatgtgtt ccggattacg
     4441 ttaccgcaaa gaaattaaca taaggcttca gtacgccata aattaaccgt gaaaaaacgc
     4501 agccacgtcg tgagtactaa cgcggctgcg attgattagc gcttacattc aaacgtaaca
     4561 gggattaacc cgcgtaagct gccagcattt cctgcgccgc cagtttaccc aacgcattcg
     4621 ctgcaaaagg actgtcgccg gtgagaactt tacggtcctt atgtactcgc ccggtgatgt
     4681 cgtcattaat aatgttcatg cccattttct tcagctcttc gccgaagtac caggtgagat
     4741 gacccggcat atagcctatc tctggcgttt gtttgtctgc ggcgtctggg aatgcgcaaa
     4801 tggaataacc attcagtggg ttatcgccgt ggcgaagcgc cagaaaagcc gccgggccgt
     4861 ggcaaaggga gataacaaaa cggtcatttt tgattgccca ctgcaaagca gcagctacgt
     4921 cctggctttc tggtaaacca ataagtgcgc catgaccacc aggaacaaag attgctgcat
     4981 attcgctgtc agcgttgagg ctggcgacaa catccgcgag tttcttcgga ttgcggaaca
     5041 acgatttatg ttgctcaaag aatggcatta ctttttcatc tttgtgcggc atagcccagt
     5101 attcaaactt ggtcatcaga ccggaaatgg tcgccacttc gaattcgaaa cctgcagcat
     5161 ggagatgata caacggcagc aacgtttcaa tcggatggtt accggtcgag aacagttttc
     5221 cgttatcggt tggcaaataa cgttcgtccg cggcgatcac cagaatttta tgtttaccgc
     5281 gatacggttt tggatagtcc acgccatcca gatcagagac aggactggta tattggctaa
     5341 gcgaatattc tgaagggaag aaggcattat cttcagcaat atcgacctgc ggacttttac
     5401 ttgtttgaac agtcatagtg tattccttat tgttgcttag ttagggtagt cactatattt
     5461 gcgactgagc tggtcattat ttttaaccag gctggaaaat tgagtcaata tatcgaaagc
     5521 ggtagtcggc gggtgtaagt atgtaatgat gatgctgcac tggtgttgaa tattattcac
     5581 gccaaatttt ccttattgag caacgtcgtc gcaagtggcc tcaatgattt tagaccatta
     5641 tcagaggtac ggggcggcgg ggagagattt agtgctgtgg aatattgtgg tgctgatgaa
     5701 tatttatatc ccggattggt ggagaaaaag ctttttccat gcactttttt tgcaaatatt
     5761 tctatatggg aagcgtgttt gttacggatt aattcataaa gaaatgcatt tgggtgttgg
     5821 cttgaagagt cgctggttga tgttattgtg ccaggagggg cagggtaagg gggtgtatcg
     5881 ataagctttg gcaattgcgt gtgccgcaga tggagatgaa atgccaataa aaaagcctga
     5941 gttcgatgag ggacgaactc aggctagtgt taagttttac agtaacacgc cgcggcgtgt
     6001 tggctgatta gaactggtag actaagccca cagccacgat atcatcggta gcgatgccgt
     6061 ttgcttcgta gaagtcatcg tcgttgtcca gcaggttgat tttgtaatca acgtaggtgg
     6121 acatgttttt gttgaagtag gaggtcgcac cgacttcaac gtatttaacc agatctttgt
     6181 tggtgtaacg cggatcgcca ttaccgtcat gagcccaacc gcctaaatcc ttacctttag
     6241 attgcaggta agcgatggac gggcgcaggc caaagtcgaa ctgatactga gcaacaactt
     6301 caaagttttg ggttttgtta gcgatggctg atcttgattc accattatca aactcgccac
     6361 caccatatgg ggtcatattg cgagtttcag cgtacatagc tgctaaatac acattattag
     6421 catcatattt tgcaccaaca gtccatgctt cagcggtttc accaccagca tttctgttgc
     6481 tatggttacg atcatttaaa ccaactccaa cctgacgatc tgaacgatca gagtttgaat
     6541 atgcagcacc cagacttaaa ccgaagtcga agtcgtagga tgttgacata ccgaaaccat
     6601 caccattttc tttggaaagg ctacggccat caccactacc ggaaccttcc tgcccgaatg
     6661 gtccaccact tttatcatta ccctgatatt gcaaagcaaa gttcaagcca tcaacctgac
     6721 caaagaagcc attgttacgg taggttgcaa caccgttggt acgaccgttc atgaagttgt
     6781 cggcacccgc gtaggtatcg ccaccaaatt ctggcagcat atcggtccat gcttcgatgt
     6841 catatacgac accgtagtta cgaccataat cgaaagtacc attctgacca aaacctaaac
     6901 ctgcgaatgc cagacgggta gcagagttat ctccatcgcc ttctgtgcca tttgcttgca
     6961 ggttgtattc ccactgaccg taaccagtga actggtcagc gatttgtgtt tcgcctttga
     7021 aaccgatacg ggcatatgac atatcgccat cagcgcccga gttgtcagag aagtagtgca
     7081 gacctgctac ttttccgtag aaatccagtt tattaccatc tttattatag acttcagccg
     7141 catttgctgc gccagcaact aataacgccg ggaccagcat tgccagaact tttcttttca
     7201 ttatgtattc ccttgtgatt ataatcttca tgaatatatc aataagtgcc gttatccaaa
     7261 aaaaagcaca tttggatac
//
LOCUS       DACBVI010000196         7246 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.197, whole genome shotgun sequence.
ACCESSION   DACBVI010000196 DACBVI010000000
VERSION     DACBVI010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.197"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..4728)
                     /locus_tag="IU325_20610"
     CDS             complement(<1..4728)
                     /locus_tag="IU325_20610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HAP5811399.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LASKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVNINGKPAARAAVTLPPP
                     PPPGAIPEIPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKDVDFPEAGEDKALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLKNLARLGKLGNLLGKSGKLLGAKLGALLGKTGKSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDIISQGATLNFAFDGDSDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTSDDRLAHSPNDRMYMLGGMSDTASNRITFERDSQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAADRI
                     IRWSDNDQTWSRFTYDEQGRCVNVTGAEGYYNATLDYGDGCTTVTDGKGIHRYYYDPD
                     GNILREAAPDGSTTMYEWDEFHHLLARHSPAGRVEKFEYNAAHGQLSRYTAADGAEWQ
                     YRYDERGLLSNITDPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTAQGLLSGIFRQD
                     ERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSG
                     LERTGTGAEGFRYDRHGNLLAYTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKD
                     TLQLTEVINPQGESYLYVLDNCGRVTEERDWGGVVWRYRYDADGLCTARVNGLEETIL
                     YSRDAAGRLAEVITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRR
                     AIEYHYPDEHTVIRCILPPEDERDRHPGESLLKTTYRYNAAGELTEVILPGDETLTFS
                     RDEAGREVLRHSNRGFACEQGWNAAGQPVSQRAGLFPAEATWGGLLPSRVREYRYDSA
                     GNVSGVTSREDYGRETRREYRLDRNGQVTAVTASGTGLGYGEGDESYGYDSCGYLKAQ
                     SAGWHRISEETDQYAGGHRLKQAGNTQYDYDAAGRMVSRTRHRDGYRPETERFRWDSR
                     DQLTGYCSAQGEQWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYS
                     VRHLVFNGFELISQQFSRVRQAHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEGKTVWR
                     PGQTSLWGLALSLPADTGYPDPRGELDPEADPGLLYAGQWQDAESGLCYNRFRYYEPE
                     TGMYLVSDPLGLLGGEQTYRYVPNPCGWVDPLGLAASSKISSLMDYIGDGRRVSGHTG
                     FLDGVRLSRSQINNIAKEMEKLGIKVIRKADKYLPPNARAAFDYGLRNIYLRKNATLY
                     EVYHEVIHAKQFAKIGREAYEALG"
     gene            complement(4748..5209)
                     /locus_tag="IU325_20615"
     CDS             complement(4748..5209)
                     /locus_tag="IU325_20615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HAP5811400.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5237..7138)
                     /gene="tssI"
                     /locus_tag="IU325_20620"
     CDS             complement(5237..7138)
                     /gene="tssI"
                     /locus_tag="IU325_20620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HAP5811401.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHHETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1339 a         2219 c         1967 g         1721 t
ORIGIN      
        1 tcctagtgct tcgtatgctt ctcgtccaat tttcgcaaat tgcttagcat gaatcacttc
       61 atgatacacc tcatataagg tagcattttt cctaagataa atattgcgaa ggccataatc
      121 aaaagctgcc ctagcatttg gtggcaaata tttatctgct ttccttatta ctttaattcc
      181 tagcttctcc atttctttag caatattgtt tatttgacta cgtgataaac gaaccccatc
      241 caggaaaccc gtatgcccac taacacgacg accatcgcca atatagtcca tcaaactgct
      301 gattttagaa cttgcagcca atcccagcgg atcaacccac ccacacggat ttggcacata
      361 ccggtaagtc tgctcacccc ccagcaaccc cagcggatca ctcaccaggt acatcccggt
      421 ttccggctcg taataccgga accggttata gcacagcccg gattctgcat cctgccactg
      481 tcccgcatac agcaggccgg ggtcggcttc cgggtccagt tccccgcgcg ggtccgggta
      541 gccggtgtct gcgggcaggc tgagtgccag cccccacagg ctggtctgtc ccggtcgcca
      601 gacggtttta ccttcactgt taaagagcat cagcggacgg cccgtcaggt cgctcaccgc
      661 atgattcgtc cgtgtcaccc actgcggggc caccgacgga tgcgcctgcc gtacccggct
      721 gaactgctgg cttatcagct caaagccgtt aaacaccagg tgccgtacgc tgtacagttt
      781 atcatcgcgg tattcccgga tttccgcaat actgtcgccg tcccacagat acgtgaagcg
      841 gattttcttc cggtcgcagc gtttctctgt tcgtctgccg ctggcgtcgt ggcggtattc
      901 ccactgctca ccctgtgcgc tgcaataccc ggtcagctgg tcacggctgt cccaccggaa
      961 ccgctctgtt tccgggcgat agccgtcacg gtgtctggtg cggctgacca tccggcctgc
     1021 ggcgtcatag tcatactgcg tgtttccggc ctgtttcagc cggtggcctc cggcatactg
     1081 gtcagtctct tcacttatcc ggtgccagcc cgcagactgc gccttcaggt agccgcagct
     1141 gtcatagcca taggactcat cgccttcgcc atagcccagc ccggtgcctg aggctgtcac
     1201 cgccgtgacc tggccgttcc ggtccagccg gtattcccgc cgtgtttccc gtccgtaatc
     1261 ttcccggctg gtgacgcctg acacattacc cgcgctgtcg taacggtact cccgtacccg
     1321 tgaggggagc agcccgcccc atgtggcttc tgccgggaaa agtccggcgc gctgactgac
     1381 aggctgaccg gctgcattcc agccctgttc acaggcaaaa ccccggttac tgtgccggag
     1441 cacttcacgt cctgcctcat cacggctgaa cgtcagcgtc tcatcccccg gcagaatgac
     1501 ctccgtcagt tctccggcgg cgttgtagcg gtacgtggtt ttcagcaggg attcaccggg
     1561 gtgtctgtcg cgttcatctt ccggtggcag gatacagcgg atgacggtgt gttcatcggg
     1621 gtagtggtat tcaatggccc gtcggccctg agtggtgaca ttcacccgcc cgcgttcgtc
     1681 atagccggtg cgctgtgatg tgccgtccgg gctgaagata cccgtcagcc tgccggattt
     1741 gtcatacgca tactgcgttt tgccttccgg cgtgatgact tctgccaggc ggcctgcggc
     1801 gtcccggctg tagaggatgg tttcctccag accgttaacc ctggcggtac acaggccatc
     1861 ggcatcataa cggtaacgcc agaccacgcc gccccagtca cgctcttccg tcacccggcc
     1921 gcagttatcc agaacataaa gatatgactc accctgcggg ttgatgactt ctgtcagttg
     1981 cagcgtgtct ttatcgtagc ggtactgcca gcggtggcct tcaccgtccg ttcgcgccac
     2041 cggcaaatca aacgggccgt actccattgt ccagacaaca ccgttaccgt ccgtgtacgc
     2101 cagcaggttg ccgtgacggt cgtagcggaa tccttccgcg ccggtgccag tgcgctcaag
     2161 gccgctcagt ttatggtgct gctgccactg caacctcact gactgaccgc cggggccgcg
     2221 catcttcacc ggcaggtcgt gaccactgta ttcggtgtgg tgttcgcggc ccatcacgtc
     2281 ggtgagtgtt tccggccggt tgtggtggtc gtactctatg cccagacgcc gttcatcctg
     2341 gcggaatatt cccgacagca gcccctgagc ggtgtatgcc agccgggtct cttcgccctg
     2401 tggcgacacc agacttaccg gcaggccgcg ttcatcacac tgctgtgtcc acgtctgccc
     2461 ggcagggtcg gtgatgttgc tgagcaggcc gcgctcatca tagcggtact gccactccgc
     2521 gccgtctgcc gccgtgtaac ggcttaactg accgtgtgcg gcgttgtatt caaatttctc
     2581 cacccgcccg gcaggggagt gacgggccag caggtgatgg aattcatccc attcatacat
     2641 ggtggtgctg ccgtccggcg ctgcttcccg cagaatattg ccgtcaggat catagtaata
     2701 acggtgaatg cccttgccgt ccgtcacggt ggtgcagccg tcaccatagt ccagcgtggc
     2761 gttgtaatag ccctccgccc cggtgacatt cacgcaccgg ccctgttcat cgtaggtgaa
     2821 acggctccac gtctggtcgt tatcggacca gcggatgatc cggtccgcag cgtcgtactc
     2881 ataaaacagg tggtagtcca gccgcgcatc cgcttctgtc agccgccccc gcgcatcctg
     2941 ttcgtaggtc gccagcgtct gtatgccgtt atccgtgcgg tgaatggctt tcaggtagcc
     3001 gctggcgtgg tacgtcagtt taagccggat cccgtcggtg tgactgacac ctgtgatccg
     3061 gtactggctg tcgcgctcaa acgtgatgcg gttgctggcg gtgtcgctca tcccgcccag
     3121 catgtacatc cggtcattgg ggctgtgtgc caggcggtca tcgctggtat cgtcagtcac
     3181 cgggtggcgc agacgcattc ccggaaaggc gtcataaaag aagcggctgc tcagtgcatc
     3241 cctgtcgaac agctccagat aatcatcgcg gcggcgcaga atgtactggg cgtgatacgg
     3301 gttaaccgcc gtatcactgt caccgtcgaa ggcgaagttc agcgtggctc cctggctgat
     3361 gatatccacc cggtttccct gttcacgcac ccaggcgtat tcgctccagg agtcgctcca
     3421 gcccacgccg gtcaggccat gcagcggcag tacagaactg tggaaacggg tgaaggagag
     3481 ggggagggtc tggcccagcg taaagtcggt acgttcgtcg cagtacgcgc cggttaccgg
     3541 atcgacagga tctgttaccc atctgatgac tttattggca atacttttta acgacttacc
     3601 tgtttttccc agcaacgcgc cgagctttgc ccccagcagc ttcccgcttt tccccagcag
     3661 gttccccagt ttgccgagtc tcgccagatt cttcagtttt cccagcagtc ccgctggcgg
     3721 cagaagatag ctggctatcg ttattccccc caggatcatt ctctgccacg gcgggaactc
     3781 tggctggata tccagatagg tcacctgttc accgccaata aagacgtccg gcgaaccttc
     3841 cacgattgcc gcgctgcact ccagtttgtc gcccttgcgc gcggcgggct gattgttgat
     3901 aaagacgttg ctgctgccct gggctatcct cggtggtttg ttctccttgt cgcagagcgc
     3961 cttgtcctca ccggcttccg ggaaatccac atcttttttg atttcgccac gcccgcccac
     4021 catggtggca aactccatta ataccggacc ggcgggaaac agaagcgatg cgccggcgct
     4081 gacgcgatcc ataaaaccgg cattgctgtc cgtcagggtg ataacagcgt tcccccaggc
     4141 cttcgccagt ggtactgctt tttccgctgc cgattcaagc aggtcagtgg caatatcacc
     4201 ccatgagggt tccccctgtg ggatttcagg tattgctcca ggtgggggag gagggggaag
     4261 ggtgacggcg gccctggcag cgggctttcc gttaatgttt acattttcag aaccggtatt
     4321 gatggcgccg tccgtgtcac aaaagctgtt tatccatgat tcaagctggt tctgtttctc
     4381 ctccacataa tcactcgcct tatagctggc atacatcagc gcaggcacca gaaccagcgc
     4441 ataagggccg gtggcacagg cggccataat caggcaacct tctgcgatgg tcactatcgc
     4501 ccccagcacg gcccctgcca gcgcccccaa aaaactcttg tgctgaattt catcgcccag
     4561 cctggcggcg ggggggccgg acttttttga tgccagcatc tgtgcaatgg ctttgtcgtt
     4621 ggctgcccgt ttcatctggc tggcgaccat cgcctgctgt gacagcattc gcattgccag
     4681 cgtaccgcct gcggtcgctc cctgtggccc gcctggtcct tcactcatgt gttatccctc
     4741 gtttatgtca gcgcggctgg tccttttgga tgtcattctc cggtggcaac ggggtgaagc
     4801 tctttatcac ctccagcagc atcgtctttt gactgttctt cagggataag gaggtgctga
     4861 aagtgaaggt cagcagcgta tctccacgcc gctggaatac ggctatctgg ctaatccgcc
     4921 cctgattccc ctcatcttcc ggaccgttcc aggaataatc cagcagccag gccggggcct
     4981 ctgccagagt gatttcccgg caggcatgtt cggtgtaccc atcaagtttg ccctggtaca
     5041 gcgccagcgt ttcctggtac agctctggtg tcagggttcc ctgtggaacg gcttcgcggg
     5101 taatgaccag gttgatgcca ctgttatctg tggacaccag gacctgcatt gactgatcct
     5161 gccattgaga cggcagttga atgtggcctt cattaaaagt gaactgcata aaatactccc
     5221 tgataatctg agtgaatcag ttgatgaaaa cagaaccgcc cacgatggtg atgccatcct
     5281 tcgacagcct gattaccgtc gattccccga cctgtagctg aatgccatcc ggcgtcagcg
     5341 caattttgct ttcctccacg gccagcaaaa tttccttaat cgccgtataa gcctgtatac
     5401 cgtcgccttc gccaccattg tttacagcgt caacatgcac cgaaccggca atcgtgcctc
     5461 tgtcctgaaa gacatcctca gataaagacc ctttcagtac tccggtgatc ttgtggcctt
     5521 tttccaccgt aagaaggcgg ttaccttcct ggatcgtgac cgtctggtca ttttttacgg
     5581 tttcgtggtg atcgtgtttt acggtggtgg tacggtcgtt gagcacttcg gtatccatgt
     5641 tcttctgcgc gtggatgtag acctgttctc tccctgtcgc atcatcaaac ttcagctcat
     5701 taaatccgct gcccatgtac gttttggagc ggatggtcat ctgcgtcttt gttcccggca
     5761 ggctgccggg ggtgcggttt tcctggtggt aggtgcgccc cataatgatc ggctggtccg
     5821 gatcgccgtt gaggaagtct acaatcacct cctgacccac acgcggtatc gccaggtggc
     5881 caaaaccggt gcctgcccac gcctgtgcca cacggatcca gcacgaactg tcctggtttg
     5941 acgggttata gcggtcccag ttgaacttca cccgcacgcg accatgttcg tcgcagaaga
     6001 tttcctcgcc tgccggtcct gtcacgacag cgctctgcgg gccgtcgacc agcggtttca
     6061 gcaacggctg tggtcgccat gttctgtctg ccgggatcac cgcaaaatgg ttctccagcg
     6121 ccgtccccgc tccctgccgt cctggcaccg cctgtggctg ttcgccgtgc agttcacttg
     6181 ccaccacctg ccattcccgg ttcaggttcg cctgcggatg ccccgtcagc acaattcgtc
     6241 gtcccggcca tatctccggc gagcgactca ttccccgcgc ggtttctgca ttgtttcgcc
     6301 agccgtccat ctgccagcgg gcaaagttct gcccgtgggc acccttgaaa cgtccggggt
     6361 agtcatacac ttcatactgc gtgcgctggt agtcctggtg ctggccttcc tgttcaaaac
     6421 gtccggccca gccggggcgt ttaaaggtgt agtctttggt caccacggaa gaagggcgga
     6481 tttgtgcgct gtagcggaac tggctgatgc agagggtgct cacctcggta cgggtgttcg
     6541 ggttccatgg gatttcaaaa gattcgggca gatggcggac tgtgtcgcac agcaccaggc
     6601 tctggtcggt acttttgtaa gcatgctcct cataaaagaa gatgccttcc tccgccgcca
     6661 tccggcacag gaaatcgtaa tcagtctcac cgtactggac acaaaactca cgggaaggat
     6721 gcggctcgct gaacagcgga ctccattcgg tcaccccgtt ttcctgcaac atcgtgccga
     6781 ggatgctttt gatgtcctcg ttctggaaga tacggaagtt ctggcgcaga ccggcacgcc
     6841 acagcggcgg gtgcaccttc atgctgtaca gcatctggtt tttgtcgttc tccccgagtt
     6901 caaaccaggt caccacgcct ttcacccggc gctgtacgtc atcgccctgc cataccgtca
     6961 ggtaggccat tttgtccagc acctgcgcaa attcaaggga gagaaactgc tggctgacca
     7021 gggagagatc gagggaaaag agcgaagaga gtgactggtt cagatgaaag gaaaccaccg
     7081 caaaagcatc cggcggcagg ccgtccactt ccagtgtgaa acgtaatccg gttgacatag
     7141 caacctccag taaaatgaat aatgatccgt accgtacaac tgcggataat gtgataagcg
     7201 ggacgacttc agacgttatt agggaaggaa cagaaaatgc tatggt
//
LOCUS       DACBVI010000197         7242 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.198, whole genome shotgun sequence.
ACCESSION   DACBVI010000197 DACBVI010000000
VERSION     DACBVI010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.198"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(304..1440)
                     /locus_tag="IU325_20625"
     CDS             complement(304..1440)
                     /locus_tag="IU325_20625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HAP5811402.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLSEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1443..1805)
                     /locus_tag="IU325_20630"
     CDS             complement(1443..1805)
                     /locus_tag="IU325_20630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HAP5811403.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2342..4255
                     /locus_tag="IU325_20635"
     CDS             2342..4255
                     /locus_tag="IU325_20635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP5811404.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4484..5632
                     /gene="mtlD"
                     /locus_tag="IU325_20640"
     CDS             4484..5632
                     /gene="mtlD"
                     /locus_tag="IU325_20640"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP5811405.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5632..6219
                     /gene="mtlR"
                     /locus_tag="IU325_20645"
     CDS             5632..6219
                     /gene="mtlR"
                     /locus_tag="IU325_20645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor MtlR"
                     /protein_id="HAP5811406.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(6231..6440)
                     /locus_tag="IU325_20650"
     CDS             complement(6231..6440)
                     /locus_tag="IU325_20650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811407.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6724..7086
                     /locus_tag="IU325_20655"
     CDS             6724..7086
                     /locus_tag="IU325_20655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HAP5811408.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1831 a         1824 c         1840 g         1747 t
ORIGIN      
        1 tttaaatgtg actgtcatcc ttcagataat aaagccatca tcgcaataga tggaaaagca
       61 ctcctgctct tttttgatta gagccgtggc agaggaacaa ctcatatcat cattgcgatc
      121 tcgacaatgt atagtctgat ccttcggcag atcaagacgg ataaaaaatc caatgagttc
      181 acactcatcc cgaacttctt aacataatgg atattaacga aaaatcatca cagccgatga
      241 aataatatgc aaaaataatt agtccttgca gggagcaaag ttccataatc tcccccgccc
      301 ccctcaatga tccaaataaa gataatgcat ccagctggtc attcttagca gcactttccg
      361 catcaccgaa acatggctga aatggtcgga gtaaaccgcc acctgggcgt agatgccgtc
      421 gggtaaggca tcgatatcat cgttagggtc cagttcaatg gttcccagca caccgtccgt
      481 gccgggcacg accgttaatg attgcaatac cccctgcgcc tgataagaac cgcctggcac
      541 gacaggtaaa atgctagtca gtttgccgtg gaacacctgc ccaggtagcg cgttaaacac
      601 cacttccgca tcatcgccgg gtttcagacg taacagcgag ttttgccgaa attgggcgac
      661 aatttgccgt ttttgctcgg ggatgaaaac catcaccgga cgcaacggca aggcagctgc
      721 gtatgtacct gggcggatca gtacctgagt aacgtagcca ttgctcggcg cgcgaatgac
      781 agtctgctca aggttatatt ttgcttcagt aagttgcgct cttaagctca caatctgcga
      841 ttgctcgccg ttaaccatac tgtcgagctg gctctggatc tgcgcctgct ccgccaccga
      901 gcctttcacc agcgcgtcct gcgcgaggaa gttttgccgc gcatcgtcga tgtcacgttc
      961 cgagaacgga ttcaccgccg cctggctgcc tttcagataa cgttgataat ttttaaacag
     1021 acggtcgcgc tccgctgaaa cctgggtggt gttggcctgc gcttcagaga gctgcgcgcg
     1081 cagcgtcttt atattatgcg tcgccgtcat caggtcagcc tgaagtctgt caactcgcgc
     1141 ctggtaacga accgggtcga gtttaaaaag cacctcgccc ttttgaataa gctgattatt
     1201 cttgtcagtg acttcagtaa caattcccgt cacctgtggc gtgatgggga tcgctatcac
     1261 tgccttttgc gcggtaaaag tgtaagggtg gttgtagttc atcaacaaaa tcaaaccact
     1321 caccagaaac acgcctccca gcgccgccgt cgccagcgtc cactgattca ccggaatgcg
     1381 gaagatttta aagaccgccc acgccagcgc cacgtaagtt aaaacaatca atagatccat
     1441 atttagatct ccgggaacgt ggaatagtca gtctttttct cggcggcgag ttgatgctcc
     1501 agccgggaaa ttcggtcaga aagtgcggcg atttccgggt cagtcgcttt ctcttgcgac
     1561 gcaacatgtg actgcatgcc ccagccgcgc tccggttgat agagcgtcgc ccagatccac
     1621 aggaacggcc agataacatg tagagtaaac aggctcaccc agcccgccgt atgaatagcg
     1681 tcggcatggg gatggttgcg ctttttggca atcagataag ggatgtcatg tatcgcgatg
     1741 attccataaa aaatcaccag aaagacgaag atcagcactc ccaacgcgaa atagtttaga
     1801 aacatatctg cctcggattc acgtttatca gtgttgtttt ttggctggca gccagaaggg
     1861 agtcaggctg atattttgac aataatccgg gttcgcgatt ctcgccataa caccaaagaa
     1921 taattttaag aggtgatgag ttgcttagtt acataacgat ttcatgacga aggcataaca
     1981 tgctgtagat cacatcaggt gaacgccgta agaaaatatc ttgtgattca gatcacaaag
     2041 attcaacaaa ccatcaaaat aaaaatgtga catcactcac atttaaatgc catttttagc
     2101 gaaaatcgcc gccttgttgc ttttttacac aagagttttg tgatgagcat cacgtcaatt
     2161 acctctctac cccctatatt tatgtgactg atatcacaca aaaggccgtc gactggacag
     2221 ttaaccgatt cagtgccaga tttcgcagta tctacaaggt ccggctacct ctgccgccac
     2281 attaacaaaa aacctcgggc ttccagcctg cgcgacagca aacataagaa ggggtgtttt
     2341 tatgtcatcc gatattaaga tcaaagtgca aagctttggt cgtttcctca gcaacatggt
     2401 gatgccaaat atcggcgcgt ttatcgcgtg gggtatcatc accgcgttat ttattccaac
     2461 agggtggtta ccgaacgaga cgctggcgaa gctggtcggg ccgatgatca cttatctcct
     2521 gccgctgctg atcggttata ccggtggtaa gctggtaggc ggcgaacgtg gcggcgtagt
     2581 cggtgccatt accaccatgg gcgttatcgt cggcgcagac atgccgatgt tcctaggttc
     2641 tatgattgca ggtccgctgg gcggctggtg cattaagcac ttcgaccgct gggtagacgg
     2701 taagatcaaa tccggttttg agatgctggt gaataacttc tccgcaggca tcatcgggat
     2761 gatcctcgct attctggcat tcctcggcat tggcccgatt gttgaagccc tgtccaaaat
     2821 gctggctgcg ggcgttaact tcatggttgt ccatgacatg ctgccgctgg cgtctatctt
     2881 tgttgaaccg gcgaaaatcc tgttcctcaa caacgccatt aaccacggta tcttctcgcc
     2941 gctgggcatt cagcagtccc atgaactggg taaatccatc ttcttcctga ttgaagctaa
     3001 cccaggtccg ggtatgggcg tgctgctggc gtacatgttc tttggtcgtg gtagcgctaa
     3061 acagtctgcg ggcggtgcgg caatcatcca cttcctgggc ggtatccacg aaatctactt
     3121 cccgtatgtg ctgatgaatc cgcgtctgat tctggccgtt atcctcggtg gtatgactgg
     3181 cgtgttcacg ctgactatcc tgggcggtgg tctggtttct ccggcatctc cgggttctat
     3241 ccttgctgta ctggcgatga caccaaaagg tgcatacttc gctaacatcg cgggtgtgtg
     3301 tgcggcgatg gctgtctcct tcgttgtctc tgccattctg ctgaaaacca gcaaagtgaa
     3361 agaagaagat gatattgaag cagcaactcg tcgtatgcag gacatgaaag ctgagtctaa
     3421 aggcgcgtct ccgctgtctg cgggcgatgt gactaacgac ctgagccacg tacgtaaaat
     3481 catcgttgcc tgtgatgccg gtatgggttc cagtgcgatg ggcgcaggtg tgctgcgtaa
     3541 gaaaattcag gatgcaggtc tgtcgcagat ttcggtcact aacagcgcga tcaacaacct
     3601 gccgccagat gtggacctgg tcatcactca ccgtgacctg accgaacgcg ctatgcgcca
     3661 ggttccgcag gcacagcata tttcgctgac caacttcctc gacagcggcc tgtacaccag
     3721 cctgaccgaa cgtctggttg ctgcccagcg ccatactgaa aacgaagtga aagtgaaaga
     3781 cagtctgaaa gacagctttg atgattccag tgctaacctg ttcaagctag gcgcggagaa
     3841 catcttcctc ggtcgtaaag cggccaccaa agaagaagcg attcgttttg ctggcgagca
     3901 gctggtgaaa ggcggttacg ttgagccgga atacgttcag gcgatgctgg atcgtgaaaa
     3961 actgaccccg acttatctgg gtgagtccat cgcggtaccg cacggtacgg ttgaagcgaa
     4021 agatcgcgta ctgaaaacgg gcgtggtgtt ctgccagtac ccggaaggcg tgcgcttcgg
     4081 tgaagaagaa gatgacattg cccgtctggt gattggtatt gctgcccgta acaacgagca
     4141 cattcaggtt atcaccagcc tgaccaatgc actggatgat gagtctgtca tcgagcgtct
     4201 ggcacacacc accagcgtgg atgaagtgct ggaactgctg gcaggtcgta agtaatccaa
     4261 tcccacctct ccacatggag aaggtggggc ttattgcctg atgcgctacg cttatcaggc
     4321 ctacaaaaca aagcgtagtt tgttgaattt gcacgttctt gtaggccgga taaggcgttt
     4381 acgcctcatc cggcgcggcc cctctccaca tggagagggt ttgggtgagg gaaaagcctc
     4441 accccagccc tctcgggtaa aaacattgat gaaggttaat actatgaaag cattacattt
     4501 tggcgcaggt aatatcggtc gtggctttat cggtaaactg ctggcagacg cgggtatcca
     4561 actgacgttt gccgatgtca atcaggtggt acttgatgcc ctgaatgccc gtcatagcta
     4621 tcaggttcat gtggtcggtg aaaccgagca ggtagatacc gtttccggcg tcaatgccgt
     4681 cagcagcatt ggtgatgatg tcgttgatct gattgctcag gttgatttag tcactaccgc
     4741 cgttggtccg gttgtgctgg aacgtattgc cccggcaatc gccaaagggc tggtgaaacg
     4801 taaagaacaa ggtaatgaat ccccgctgaa catcatcgct tgtgaaaaca tggtacgcgg
     4861 caccacgcag ctgaaaggcc atgtgatgaa cgccctgccg gaagatgcca aagcgtgggt
     4921 agaagaacac gttggctttg tcgattccgc cgttgaccgc atcgtgccgc cttcggcttc
     4981 tgcaaccaac gatccgctgg aagtgacggt agaaacattc agcgaatgga ttgttgataa
     5041 aacgcagttc aaaggcgcgc tgccgaacat cccaggcatg gagttaaccg acaacctaat
     5101 ggcatttgtc gaacgtaaac tcttcaccct gaacacgggt catgctataa ccgcgtacct
     5161 cggaaaactg gccggtcatc agaccattcg tgatgcgatt ctcgacgaga aaatccgcgc
     5221 ggtggtaaaa ggtgcgatgg aagaaagcgg tgcggtactg atcaagcgct acggctttga
     5281 cgcggacaaa catgcggcgt acatccagaa aatcctcggt cgtttcgaga acccgtatct
     5341 gaaagatgat gtagagcgcg taggccgtca gccgctgcgt aaactgagtg ctggcgaccg
     5401 tctgatcaag ccactgctcg gaacgctgga atatggtctg ccacataaaa acctgattga
     5461 aggtattgcc gctgcaatgc acttccgcag tgaagatgat ccgcaggctc aggaactggc
     5521 agcgctgatt gccgacaaag gcccacaagc cgcgctggca cagatttccg gtcttgatgc
     5581 caacagcgag gttgtatccg aggcagtaac cgcttataaa gcaatgcaat aatggtggac
     5641 caggcgcagg ataccctgcg cccgaataac agattgtcag atatgcaggc aacaatggaa
     5701 caaacccagg cctttgaaaa ccgtgtgctt gagcgtctga atgctggcaa aaccgtgcga
     5761 agctttctga tcaccgccgt cgagcttttg accgaggcgg taaatcttct ggtgcttcag
     5821 gtattccgca aagacgatta cgcggtgaag tatgctgtag aaccgttact cgacggcgac
     5881 ggtccgctgg gcgatctttc tgtgcgttta aaactcattt acgggttggg tgtcattaac
     5941 cgccaggaat acgaagatgc ggaactgctg atggcattgc gtgaagagct aaatcacgac
     6001 ggcaacgagt acgcgtttac cgacgacgaa atccttggac cctttggtga actgcattgc
     6061 gtggcggcgt taccaccgcc gccacagttt gaacctgcag actccagttt gtatgcaatg
     6121 caaattcagc gttaccaaca ggctgtgcga tcaacaatgg tcctttcact gacagagctg
     6181 atttccaaaa tcagcttaaa aaaagccttc caaaagtaag caacgcctgc ttactgcccc
     6241 tctacctgct tcggccgata aagccgacga taatactcca gacgttgtag atataacggc
     6301 acgctttcat caggtattcc ggacggaatc gcgttacggg gaggtagttt tttcagatac
     6361 tcccggaacg cctggctcga tgccatgaaa tctacggctt tatcgataag aagcggaacg
     6421 ttttcaccta atttgcccat gatgatatct ccgtattacc ccgcgccggg aatgcgcggc
     6481 cgccaatttt agtttagatc ccggtataaa tgacattaag gaaaacgtgc tcaatcctca
     6541 ataatcatcc gcttttgtta actatctttt ttctttaaat ttcttaatta atcaataaat
     6601 tgcgtagttt ttaagcatgt taatgtttac gttatagaac tattgcaatg tcatcagttg
     6661 tgaacaaagc acctggtcgc gcatactagg ggctataaat ttatctttat cagaagccat
     6721 cacatgaaag aagtcgaaaa aaacgaaatc aaacgtctca gcgatcgtct ggacgccatc
     6781 cgccaccagc aggccgatct gtcgctggtt gaagccgcag acaaatatgc cgaactggaa
     6841 aaagagaaag ccactctgga agcagaaatt gctcgcctgc gcgaagttca cagccagaaa
     6901 ctcagtaagg aagcacagaa gctgatgaag atgccgttcc agcgcgcgat tactaaaaaa
     6961 gagcaagctg atatgggcaa actgaagaag agcgttcgcg gactggtcgt tgtgcaccca
     7021 atgaccgcgc tgggccgcga aatgggccta caagagatga cagggttctc taagactgcg
     7081 ttttaagttc ctttctcgtc gctgtaatta cagccactca cgatttactc gctcctgctg
     7141 gcacatgcag gagcctgttg tagagatcga atatttataa cgttcttaac attatatatt
     7201 taactccacc tacacttctg agcgcccgga gcaggctaaa tt
//
LOCUS       DACBVI010000198         7222 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.199, whole genome shotgun sequence.
ACCESSION   DACBVI010000198 DACBVI010000000
VERSION     DACBVI010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.199"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..4618
                     /locus_tag="IU325_20660"
     CDS             <1..4618
                     /locus_tag="IU325_20660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
                     /protein_id="HAP5811409.1"
                     /translation="IGGNATGIGTLNVQDQDSVITVRRLYNGYFGNGKVNISNNGLIN
                     NKEYSLVGVQDGSHGVVNVTDKGHWNFLGTGEAFRYIYIGDAGDGELNVSSEGKVDSG
                     IITAGMKETGTGNITVKDKNSVITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGE
                     TGVGKVSITTGGMWEVNKNVYTTIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTL
                     NLMDATSLFDTVGINVGNFGSGIVNVSNGATLNSTGYGFIGGNASGKGIVNISTDSLW
                     NLKTSSTNAQLLQVGVLGAGELNITTGGIVKARDTQIALNDKSKGDVRVDGQNSLLET
                     FNMNVGTTGTGTLTLTNNGTLNVEGGEVYLGVFAPAVGTLNIGTAHGEAAADAGFITN
                     ATKVEFGLGEGVFVFNHTNNSDAGYQVDMLITGDDKDGKVMHDAGHTVFNAGNTYSGK
                     TLVNGGLLTIASHTADGVTGMGSSEVTIASPGTLDILASTNSAGDYTLTNALKGDGLM
                     RVQLSSSDKMFGFTHAAGTEFAGVAQLKDSTFALERDNTAALTHAMLQSDSENTTSVK
                     VGEQSIGGLAMNGGTLTFDTDIPAATLAEGYISVDTLVVGAGDYTWKGRNYQVNGTGD
                     VLIDVPKPWNDPMANNPLTTLNLLEHDDSHVGVQLVKAQTVIGSGGSLTLRDLQGDEV
                     EADKTLHIAQNGTVVAEGDYGFRLTTAPGDGLYVNYGLKALNIHGGQKLTLAEHGGAY
                     GATADMSAKIGGEGDLAINTVRQVSLSNGQNDYQGATYVQMGTLRTDADGALGNTREL
                     NISNAAIVDLNGSEQTVEAFTGLIGSTVLFKEGSLTVNKGGISQGELTGGGNLNVTGG
                     TLAIEGLNARYNALTSVSPNAEVSLDNTQGLGRGNIANDGLLTLKNVTGELRNSISGK
                     GIVSATARTDVELDGDNSRFVGQFNIDTGSALSVNEQKNLGDASVINNGLLTISTERS
                     WAMTHSISGSGDVTKLGTGILTLNKDSAAYQGTTDIVGGEIAFGSDSAINMASQHINI
                     HNSGVMSGNVTTAGDVNVMPGGTLRVAKTTVGGNLENGGTVQMNSEGGKPGNVLTVNG
                     NYTGNNGLMTFNATLGGDNSPTDKMNVKGDTQGNTRVRVDNIGGVGAQTVNGIELIEV
                     GGNSAGNFALTTGTVEAGAYVYTLAKGKGNDEKNWYLTSKWDGVTPADTPDPINNPPV
                     VDPEGPSVYRPEAGSYISNIAAANSLFSHRLHDRLGEPQYTDSLHSQGSASSMWMRHV
                     GGHERFRTGDGQLNTQANRYVLQLGGDLAQWSSNAQDRWHLGVMAGYANQHSNTQSNR
                     VGYKSDGRISGYSAGLYATWYQNDANKTGAYVDSWALYNWFDNSVSSDNRSADDYDSR
                     GVTASVEGGYTFEAGTFSGSEGTLNTWYVQPQVQITWMGVKDSDHTRKDGTRIETEGD
                     GNVQTRLGVKTYLNSHHQRDDGKQREFQPYIEANWINNSKVYAVKMNGQTVSRDGARN
                     LGEVRTGVEAKVNNNLSLWGNVGVQLGDKGYSDTQGMLGVKYSW"
     gene            4802..5068
                     /gene="hipB"
                     /locus_tag="IU325_20665"
     CDS             4802..5068
                     /gene="hipB"
                     /locus_tag="IU325_20665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HAP5811410.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            5068..6390
                     /gene="hipA"
                     /locus_tag="IU325_20670"
     CDS             5068..6390
                     /gene="hipA"
                     /locus_tag="IU325_20670"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HAP5811411.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGAGIHISDLKLAMGVNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK"
BASE COUNT         1983 a         1557 c         1957 g         1725 t
ORIGIN      
        1 tatcggtggc aatgccaccg gtatcggaac cctgaatgtg caggatcaag actctgtcat
       61 cacggtacgc agactctata atggatattt cggtaatggc aaagtcaata tttccaataa
      121 tggactgatt aataacaaag aatattcatt ggtgggcgtt caggacggtt cccacggtgt
      181 cgtcaacgtg actgataaag ggcattggaa tttcctcgga acgggcgaag ctttccgcta
      241 tatctatatc ggtgatgctg gcgatggtga acttaatgtc tcgagtgaag gcaaagtaga
      301 ttcgggaatt atcactgcgg ggatgaaaga aacaggcaca ggcaacatta ctgttaagga
      361 taagaactcc gttatcacta atctcggaac taatcttggt tatgacggcc acggcgaaat
      421 gaatatcagt aatgaggggc ttgttgtcag caacggagga agctcactcg gttatggaga
      481 aaccggcgtc gggaaggtta gcatcaccac gggggggatg tgggaggtca ataagaatgt
      541 ctataccact attggtgttg cgggcgtcgg aaacctcaat atcagcgatg gcggtaagtt
      601 cgtatcgcaa aatattactt ttttgggcga taaagcaagc ggtatcggca cactgaacct
      661 gatggatgcg acatcattgt tcgatactgt aggtatcaat gtcggtaatt ttggtagcgg
      721 catcgtaaat gtcagtaatg gtgccactct taattcaacg ggctatggat ttatcggagg
      781 aaatgcctcc ggtaagggga tagttaatat ttcaacggat agtctctgga atttaaaaac
      841 gtcatctact aacgcccaat tgctacaggt cggtgtatta ggcgcgggtg aactgaatat
      901 taccaccgga ggtatagtta aagcgcgtga tacacaaata gctctcaatg acaaaagtaa
      961 gggcgacgtg cgggtggatg ggcagaactc tcttcttgaa acattcaata tgaacgtagg
     1021 gacgactggc acaggtacgt taaccctgac gaataacggt acgctgaatg tcgaaggtgg
     1081 agaagtttac ttaggtgttt ttgcgcctgc tgtaggaacg ctaaacattg gtactgctca
     1141 cggtgaggcg gcggcagatg ccggatttat caccaatgcg acgaaagtgg agtttggtct
     1201 tggcgaaggc gtttttgtct ttaatcatac caataacagt gatgccggct accaggtcga
     1261 tatgctgatt acgggtgacg ataaagacgg aaaagtgatg catgatgcag gccatacggt
     1321 gttcaatgca gggaatactt atagcggtaa aacgttggtc aatggcggcc tcctgaccat
     1381 tgcgtcacat acggcagatg gggtaactgg catgggttcg agtgaagtaa ccattgcaag
     1441 ccccggtacg ctcgacattc tcgcatcaac gaacagtgcg ggagattaca cgctgaccaa
     1501 tgcgctcaaa ggcgatggct tgatgcgagt gcagctgtca tcctccgaca aaatgtttgg
     1561 ctttacccat gcagcaggga ctgaattcgc cggtgttgcc caactgaaag acagtacctt
     1621 cgcactggaa cgcgacaaca ccgctgcgct tactcacgcg atgctgcagt ctgacagtga
     1681 aaataccaca tcggtaaaag tgggagagca atccattggt ggactagcca tgaatggcgg
     1741 tacgctcact ttcgatacgg atatacctgc tgcgacgctt gcggagggat atatcagcgt
     1801 cgatacactg gttgtcggcg cgggtgacta cacctggaaa ggccgtaact atcaggtaaa
     1861 cgggacgggc gacgtgctta tcgacgtgcc taaaccgtgg aatgatccca tggcgaataa
     1921 ccctctgacg acgctcaatt tgctggaaca cgatgatagt catgtcggcg ttcaactggt
     1981 gaaggcgcaa acggttattg ggtcgggtgg ctcattaacg ttacgtgatt tacagggcga
     2041 cgaggtggaa gcggacaaaa cgttacatat tgcgcaaaac ggaacggtgg tcgccgaggg
     2101 tgattatgga ttccgcctca cgaccgcacc aggtgatggt ttgtacgtta actatgggct
     2161 gaaagcgctg aacatccatg gtgggcaaaa gctgacgtta gccgaacatg gcggagccta
     2221 tggcgcaacg gccgatatgt cggcaaaaat cggtggtgaa ggggatctgg caatcaatac
     2281 ggtgcgacag gtttcgcttt ccaacggtca gaacgactat cagggggcaa cctacgttca
     2341 gatggggaca ttacgtaccg atgcggatgg tgcacttggc aacacccggg aactgaacat
     2401 cagcaacgcg gccatcgtcg atcttaatgg atcggagcag acggtagagg cattcaccgg
     2461 actgataggt tcgactgttt tgttcaaaga gggttcgctg acggtgaata aaggtgggat
     2521 cagtcagggc gaactgacag gtggcggaaa cctgaatgtt acaggtggaa cactggctat
     2581 cgaggggctt aatgcacgct acaatgcgtt aaccagcgtt agcccaaatg cggaagtcag
     2641 cctcgataat acgcaggggt taggcagagg aaatattgcc aatgacggtc tgttaacgct
     2701 aaaaaacgtg actggcgaac tgcgtaatag cataagcggg aagggtatcg tgagcgcaac
     2761 agccaggaca gacgtagagc tggatggcga taatagccgc tttgtggggc aattcaacat
     2821 tgatacaggc agcgcgctca gcgtcaacga gcagaaaaac ctgggtgatg cttccgttat
     2881 caataatggc ctgctcacca tctccactga gcgtagctgg gcgatgacgc acagtatcag
     2941 cggtagcggt gatgtgacaa aactgggtac cgggatcctg actcttaaca aagattccgc
     3001 ggcgtatcag ggtacgacgg atatcgtggg tggtgaaatt gctttcggtt ccgactctgc
     3061 cattaatatg gcaagtcagc acattaatat ccataacagc ggtgtgatgt cgggaaatgt
     3121 caccactgca ggtgatgtga acgttatgcc aggggggaca ctgcgtgtcg ctaaaaccac
     3181 tgtcggcggt aacctggaga atggtggcac ggttcaaatg aacagcgaag gggggaaacc
     3241 ggggaatgta ctgaccgtta acggtaacta taccggaaac aatggcctga tgacgttcaa
     3301 cgcgacgctg ggcggcgata attcgcccac cgataagatg aacgtgaaag gcgataccca
     3361 agggaatact cgcgttcggg ttgataacat tggcggcgtc ggtgcacaaa cggtcaacgg
     3421 tattgaactc attgaggtcg gcggtaattc tgcaggtaac ttcgcgctga ccaccggaac
     3481 tgtcgaagct ggggcttacg tctacacgct ggctaaaggg aaggggaatg acgagaaaaa
     3541 ctggtatctg accagtaaat gggatggcgt aacgccagcg gatacacccg atcccatcaa
     3601 taatccccct gttgtggatc cggaaggccc atcagtttat cgcccggagg ccggaagcta
     3661 tatcagcaac attgccgcag ccaactcgct gtttagccat cgcttacacg accgtctggg
     3721 tgagccgcag tatacagatt cactgcattc tcagggttcg gcaagcagta tgtggatgcg
     3781 tcatgtcggg gggcacgaac gtttcaggac cggtgacggc cagctaaata ctcaggctaa
     3841 ccgctatgta ttgcagctag gcggcgattt ggcgcagtgg agtagcaacg cgcaggatcg
     3901 ctggcatctt ggcgtgatgg caggctacgc caatcagcac agtaatactc agagtaatcg
     3961 tgtgggttat aaatcggatg ggcgcatcag cggttacagc gcaggactgt acgcgacctg
     4021 gtatcagaac gatgcgaata agaccggcgc ttatgttgac agctgggcgc tgtataactg
     4081 gtttgataac agcgtcagtt ccgataaccg ttctgctgac gactatgatt ctcgcggtgt
     4141 gacggcctct gttgagggtg ggtatacctt tgaagcggga acttttagcg gcagcgaagg
     4201 gacgctgaat acctggtacg tccagccaca ggtacaaatc acctggatgg gtgtgaaaga
     4261 ttctgaccat acccggaaag acggaacacg cattgaaacg gaaggcgacg gaaacgtgca
     4321 aacgcgactt ggggtgaaaa cctacctgaa cagccatcac cagcgtgacg atggtaaaca
     4381 gcgtgagttc cagccttaca ttgaagcgaa ctggatcaac aacagcaaag tctacgcagt
     4441 gaagatgaat ggtcaaaccg taagccgtga tggtgcgcgt aatctcggtg aagtacgtac
     4501 cggggttgag gcgaaagtaa ataacaacct tagcctgtgg gggaatgtcg gtgtgcaact
     4561 aggtgataaa ggctatagcg atactcaggg catgctggga gtgaaatata gctggtaaac
     4621 cgtataagcc gcatgtcgag atggcatgcg gcttaagcct tccttttatc cgcgatcgcg
     4681 gatatcgcag cgtttatccc gtacagcgga taagatgtgt ttcctgattg actttatccc
     4741 cactaaagga taaaactgat aatatcccct tgagcggata aatatgctgt ggacgtatga
     4801 catgatgagc tttcagaaga tctatagccc aacgcaatta gcgaatgcaa tgaaactggt
     4861 tcgtcagcaa aatggctgga cgcagagcga gctggcgaaa aaaatcggca ttaagcaggc
     4921 gacaatttcc aatttcgaaa acaaccctga caataccacg ctgacgacat tttttaagat
     4981 tttacagtcg cttgaactct caatgacgct atgcgacacg aaaaatgcct cgccagaatc
     5041 aacagaacag caagatctgg agtggtaatg cctaaacttg tcacttggat gaacaaccag
     5101 cgggtaggcg agttaacgaa gttagccaat ggcgcgcaca cctttaagta tgcaccggag
     5161 tggttagcca accgttatgc cagaccgtta tcactttcgc tgccattgca gagggggaat
     5221 atcacctctg atgccgtatt taacttcttc gataacctgt tacccgatag cccgattgta
     5281 cgtgaccgga tcgttaaacg ttatcatgcc aaatccagac aaccgtttga tttattgtca
     5341 gaaatagggc gagacagcgt tggtgccgtg acgttaatac ccgaagatga aaccgtaacg
     5401 catccgataa tggcatggga aaagcttact gaagccagac ttgaagaagt attaacggct
     5461 tataaagcag atatccctct gggcatgatt agagaagaaa atgactttcg catctcggtt
     5521 gctggcgcgc aggagaagac agcgctgctc agaataggca atgactggtg cattccgaaa
     5581 ggaataacgc cgacgacgca catcattaaa ttaccgattg gcgaaatcag gcagcccaat
     5641 gcgacgctcg atctcagcca aagcgttgat aatgagtatt actgtctgct gctggcgaaa
     5701 gaacttgggt tgaatgtgcc ggacgcagaa atcattaaag cggggaatgt gcgcgcgtta
     5761 gcggtcgaac gttttgacag gcgttggaat gctgagcgaa cggttttact tcgcttgcca
     5821 caggaggata tgtgtcagac attcggttta ccttcatcgg tgaaatatga atcagatgga
     5881 ggcccaggca tcgcgcggat catggctttt ttgatggggt ccagcgaggc gctgaaagat
     5941 cgctatgatt ttatgaagtt tcaggtgttc cagtggttga ttggcgcaac ggatggccat
     6001 gcaaaaaact tctccgtatt tattcaggct ggcggcagtt atcgactcac gccattttac
     6061 gacatcattt cagcatttcc ggtattgggt ggcgcaggaa tacacatcag cgatctcaaa
     6121 ctggcaatgg gggttaacgc atccaaaggc aaaaaaacgg caatcgataa aatttatccg
     6181 cgacattttc tggcgacagc aaaggtgctg aaatttccgg aagtgcagat gcatgaaatc
     6241 ctgagtgact ttgccagaat gattccggca gcactggata acgtgaagac ttcattaccg
     6301 acagattttc cggagaacgt ggtgacggca gttgaaacca atgtgttgag gttgcacggc
     6361 cggttaagcc gagaatacgg tattaagtaa gatatggggt tttggtcgtt gttaatgagc
     6421 aggacaatca tgaccgccaa cgactggatt ctcccttggt ttatgtttgt caaagctcag
     6481 tgaaaatgtc taatcactgg ctcattagaa atgtccatat gttgaaaagg ccgctgcgaa
     6541 aatccctccc gcaacggcct ctttagcata acgtcattat gcgaaccaat tatggtttga
     6601 gtttatactt ctgaccatta atgatttttt tgattaactc gaatgcagcg ggggaatatt
     6661 tatgaacacc ttccttcccc gttttcacta gcacatgata gttattatct gaggatttaa
     6721 tattaaaacc agtttccttg tgtattcttt ctaatacttt atggacgtaa tgccaagatc
     6781 ccatatcaag taattttgca agttcagata tggtgtaggg atagcttgcg ttaagtgctt
     6841 gcccattatt taactctgca attccataaa gtttcgctaa cacgttatct tgctcagcaa
     6901 tgttcttaag catttcaaaa tcatatggaa gctcttgttt tacaagagat tgagaggcaa
     6961 atgtataggt tctggctaat agcgccctga ctaattttgg gttaacactg atttctggcg
     7021 tatttgacga tagggcatca taaatccaac cgaaatcatt ggcatagatc actttgattc
     7081 ggatgctttt attttcacca acaccaatca aaatctcttt ctggtgtgaa cctgtacctt
     7141 cacattctgg actaaaggaa accaaataaa tattcggaat caaagcgtta tttggcgcaa
     7201 ttatctcatc aatatcagac ag
//
LOCUS       DACBVI010000199         7222 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.200, whole genome shotgun sequence.
ACCESSION   DACBVI010000199 DACBVI010000000
VERSION     DACBVI010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.200"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(196..1674)
                     /locus_tag="IU325_20675"
     CDS             complement(196..1674)
                     /locus_tag="IU325_20675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011191788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HAP5811412.1"
                     /translation="MERYFIGVDLGGTETKAGIYTAEGREIGVSSQVLPLLSPCPGFC
                     ERDMEALWKVTCQVISLSMSENNITASQIDGISFSAHGKGLYLLDRQGKPLRHGIVSS
                     DSRAQQLVASWKEQGIDRESYHRSLQQLWPSHPVALLHWLKKHETDNYGRIGTILMVH
                     DYIRSRLTGKLACEETNISGSNFYNQYTSAYDAELFRLFEIKEVAGKVPDIIGSADLA
                     GVVTSEAASQCGLLAGTPVFGGVFDVVGAALTSGIYDSTTLSAVAGTWSIATRIFDRI
                     IPSDYPYVWGKFSIPGTFFAHEGSPTSASNLTWFLQQFLPDLPDSYRVINEWVERGYE
                     KQNDILFFPWLFGSNYHEALSGGFLGLCGHHKREDLVYAIYQGIVFSHLLHQDRLIEI
                     DKSLQTIRFSGGPTHSRIWMQMFCDASNMPLEIVDIEQSGCRAAAMCAAVGGGAYSGF
                     KQAMKATHLNVIRLEPNVEKAHMLRRRFARFKQVASALSSVV"
     gene            complement(1691..2554)
                     /locus_tag="IU325_20680"
     CDS             complement(1691..2554)
                     /locus_tag="IU325_20680"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP5811413.1"
                     /translation="MRRHPLGIYEKALPRNTSWVEKLALAKSCGFDFVEMSVDENDER
                     LARLDWSLTERMELISAIQQTGIRIPTMCLSAHRRFPFGSHDRDTRQQARTIMLKALR
                     LAQDLGIRTIQLAGYDVYYEPQNEETIAWFEDAMAWTAEQAAAAQVMCAVEIMDTPFI
                     NSISKWKVLDTKINSPWFTVYPDVGNLTAWGNELESELVKGIDRIAAIHLKDTYPVTP
                     DSPGQFRDVPFGEGCVDFIKLFKILRNLNYRGTFLIEMWTEKATEPLLEIIHARQWIE
                     EKMRLAGWDNI"
     gene            complement(2554..3600)
                     /locus_tag="IU325_20685"
     CDS             complement(2554..3600)
                     /locus_tag="IU325_20685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP5811414.1"
                     /translation="MNKRIFFPLDGTRSGLLSICFIVVVLFSLAMPGRFFTDNTFLSI
                     AFQLPELGLLTFAMFIPMLSGGLNLAIIGTANLTSLFMAWVLIQYVPADASTGSQLFW
                     LFLAIVGAMLIAIIIGAITGLIISHIGAHPILVTLGSMTIISGIGIYLTKGAALSGMP
                     PVVRSIGSEVIMGIPVPMMIFIVTTVALALFLGKTRTGKTIYMCGSNINATWFSGIRT
                     DRVLIAIYAISSMLCVLAGLIMLARFNSARMGYGDSYLLLTVLAIVLGGTDPFGGAGK
                     VSHVFCALLVLQVIATGLSLLGLSLHFNLAVWGITLIAALAFKFIKQKWQLQRDMNKS
                     RKIFQQQLITREGK"
     gene            complement(3614..4606)
                     /locus_tag="IU325_20690"
     CDS             complement(3614..4606)
                     /locus_tag="IU325_20690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012105633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP5811415.1"
                     /translation="MVSFMRLRFPRSVEAWLGWVIVLMLVFFSIMSDEFLSIQNLLDL
                     SESYAVTGIFALGLFVVLVTGGIDISFAAVASVVQYVVASLLLNETLSNPFFCLVFAV
                     FFGIIFGLINAILIYYLNVVSIIITISMQSLLFGMLMWLTNGHSIYDLPDWWITQRSF
                     LSFEFGGEDYQIGLPLVVMLGMAFLTWVIMNKTHIGRQLYAVGGNTESARRIGIRVSL
                     IYLFAYGYLGAAAAIGGMLQAYRMSEVVPNALVGGELDVLAAAVLGGASLSGGRGTVI
                     GTLMGVFLIGILKNGLNLIGVSSYFVNIVIGVVIVSAICVTHYKKRKETDVGFV"
     gene            complement(4599..6116)
                     /locus_tag="IU325_20695"
     CDS             complement(4599..6116)
                     /locus_tag="IU325_20695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP5811416.1"
                     /translation="MMPHIIEKNAEQPLVTLRSLTKSFGGHRALKNINLTLNKGEVHC
                     LAGANGCGKSTLIKTLCGVYVPDSGSEIIIDGNSWTRLSPDKARELGIQVIYQDLSLF
                     PNLSVAENIAFEYNLKGYLGLHRKKQLRDVSQKIIQELEFNLDPDALVQHLPIAQRQQ
                     VAICRALIADARLVIMDEPTASLTRTEVNQLLRTVNYLRDKNITVVFVSHRLDEVKEI
                     SDRITVIRDGEIIGTWAANQLSPGDITELMTGKTIVHQKKLPNSVEGKVVLELKKLCR
                     KGQFEDVSLKLHQGEVLGLCGLLGAGRTELALSLFGITRADSGDIYLEGKPVTIKDNT
                     HAVKLGIAYVSEDRLTLGAVLPQSISDNMVLSILDRLKTPSGFIDENRKETLVKEWIQ
                     DLDIKVTDPNNPLSTLSGGNQQKVVLAKWILTRPKVLILDAPTVGVDIGAKDSIYQLI
                     HRLSGVGISVILITDEAPEAYYNCDRIFHMKRGKIIHELLPSNYTEQQLAEVING"
     gene            complement(6180..7184)
                     /locus_tag="IU325_20700"
     CDS             complement(6180..7184)
                     /locus_tag="IU325_20700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP5811417.1"
                     /translation="MKKLILPCLIATVLSSTAAWADQTVTSQKANKPFTMGVVVKVGG
                     IPWFNVMEQGIKDEGKLLGVDAWQVGPTTADPAEQVRAIEDLIARKVDVIGVVPNDAK
                     VLEPVLKRAQEAGIKVITHESPDQKNADWDFELLNTQTMGANHMKDMAKCMGEEGKYA
                     MFVGSLTVPLVNDWANAAIAYQKEHYPNMTLVEDRFGVAESVDDSMRTTNDLMSKYKD
                     LKGIMSFGSQGPIGAGRAIDKRKKNDAICVFGTFTPGQGIKLLEKGAIDGGYISNPMI
                     AGKVFVQVATAMMNGEPIKDGVKIGDMGEIKVNNNTIYSDNPEKLDLENTRHLVKLGL
                     "
BASE COUNT         2173 a         1812 c         1350 g         1887 t
ORIGIN      
        1 tcggaatgag agatgaaaag aagatacaca aacaggaaga aatacgatta atattggtcg
       61 cggtaagggt agccgttcct cccttccgca acccattaac ccttgaagtc acagtctatt
      121 tcaggcattt ctcaactcaa aataacgaag gccaatgtga actttgtggc tctccagaat
      181 ccgctttgtt ttccatcaaa caacggaact cagcgcactg gcaacctgtt taaagcgagc
      241 aaaccgtcgc ctgagcatgt gtgctttttc tacattcggt tcaagacgaa tcacgttgag
      301 atgcgtggcc ttcatggcct gtttgaatcc agaataagcc cctcctccta ctgccgcaca
      361 cattgcagcc gctctacaac ctgactgttc aatatcgaca atttcaagag gcatgttgct
      421 ggcatcacaa aacatctgca tccagattct ggaatgcgtt ggcccgccag agaaacgaat
      481 cgtttgcaat gacttatcaa tctcaataag acggtcctga tgcaacaaat gagaaaagac
      541 aatgccctga taaatcgcgt acaccaaatc ttctctttta tgatgcccac acagccccaa
      601 aaatcccccg cttaatgcct catggtaatt cgagccaaac aaccacggga aaaagagaat
      661 atcattctgc ttctcatacc ctcgttccac ccactcatta atcacccgat agctgtcagg
      721 aagatccggt aaaaattgct gcaagaacca tgtcaaatta ctggctgatg tagggcttcc
      781 ctcatgggcg aaaaatgtac ctggaatgct aaatttcccc catacatagg gataatcaga
      841 aggaataatg cgatcaaaga ttcgcgttgc aattgaccag gtgccagcaa ccgcacttaa
      901 cgttgtactg tcatagatgc cagaagtgag cgccgcacca acgacatcaa aaacaccacc
      961 aaaaacaggc gttccggcga gaagcccaca ttggctggct gcttcagatg tcacaacccc
     1021 tgctaaatca gcggagccaa taatatcggg aaccttaccc gcaacttcct taatctcaaa
     1081 taggcgaaaa agttcagcat cataagcact ggtatattga ttataaaaat tactgcctga
     1141 aatattggtt tcttcacaag ccagtttgcc agtaagccgt gaacgtatgt aatcatgaac
     1201 catcaaaatg gttccgatgc ggccataatt gtcagtctca tgttttttca accaatgcaa
     1261 tagtgctaca gggtgcgatg gccagagctg ttgcagacta cggtgatagc tttcgcggtc
     1321 aattccttgt tctttccagc ttgcaaccag ttgttgtgcg cgagaatcag aggaaacaat
     1381 gccgtgtcgt aagggcttac cttgccggtc gagcaggtac aaacctttac catgagcaga
     1441 aaaactgatg ccatcaatct gactcgcagt aatattgttc tctgacatcg acagtgaaat
     1501 gacctggcac gttaccttcc ataacgcctc catgtcgcgt tcgcaaaaac caggacatgg
     1561 gcttaacaga ggtaacactt gcgaagagac acctatttca cgtccttctg ccgtataaat
     1621 tcccgctttg gtttctgtac cacctaaatc gacaccaata aaataccgct ccatgacccc
     1681 tccttagttt ctaaatatta tcccagccgg caaggcgcat tttctcttca atccattgac
     1741 gtgcatgaat aatttctaat agaggttccg tcgctttttc tgtccacatt tctatcagaa
     1801 acgttccgcg ataattaaga ttacgcagaa tcttaaataa tttaataaaa tctacacacc
     1861 cttcaccaaa aggaacgtca cgaaattgcc ctggagaatc aggcgttact gggtacgtat
     1921 ctttaagatg gattgcggca attcgatcaa tcccctttac cagctcactt tccagttcat
     1981 taccccatgc ggtcagattg ccaacatccg gataaacggt aaaccaaggg gaattaattt
     2041 ttgtatccag gactttccat ttactaatag aattaataaa tggcgtatcc atgatttcta
     2101 cagcacacat aacttgtgcc gctgcggcct gttcagctgt ccaggccatc gcatcttcaa
     2161 accaggcgat agtttcttca ttctgcggct cataataaac gtcatagcct gccagttgga
     2221 ttgttcgaat ccccaaatcc tgcgccaggc gtaacgcctt caacataatc gtacgcgcct
     2281 gctgacgagt atctctatca tggctaccaa atggaaaacg ccggtgagca gacaggcaca
     2341 ttgtcggaat acggatcccc gtttgctgaa tggcgctaat aagctccata cgctcggtga
     2401 gcgaccagtc caggcgggcg agtcgttcat cattctcatc taccgacatc tcaacaaaat
     2461 caaaaccgca agatttagcc agggctagtt tctctaccca actggtattc ctgggtagcg
     2521 ctttttcata aatacctaac gggtggcgtc gcattatttt ccctcacgag tgatcaattg
     2581 ttgttgaaag attttccggc tcttattcat gtcacgctgc aactgccatt tttgtttgat
     2641 gaatttaaat gccaatgcgg cgatcaatgt gatcccccat accgccagat tgaaatgcaa
     2701 actaagtccc aacagactaa ggccggtagc aataacctga agcacaagta aggcacaaaa
     2761 aacatgactg actttacccg ccccaccgaa tggatctgtg cctcccaata caattgcaag
     2821 aaccgtcaag agcaaatagg aatcgccata gcccatacgc gccgaattaa atcgcgcgag
     2881 cataataaga cctgctaata cacacaacat gctggatata gcatatatag caattaatac
     2941 gcggtcggtg cgaataccgc taaaccatgt ggcgttaata ttactgccac acatataaat
     3001 cgtttttccc gtacgggttt tgccgaggaa cagagctaaa gcaacggtgg tgacgataaa
     3061 aatcatcatc ggtactggga tccccatgat aacttcagac ccaatactgc ggactactgg
     3121 tggcatacca cttaaagctg cccccttagt caggtaaata ccgataccac taataatggt
     3181 catgctaccc aatgtgacca aaatagggtg agcgcctata tgagaaataa ttaacccagt
     3241 aatagctcca atgatgatgg caataagcat cgccccgaca atagccagga agagccaaaa
     3301 caactgacta cctgtagatg catctgcggg tacatattga atcaataccc aggccataaa
     3361 caggctggtc agattcgcag taccgatgat tgccagatta agccccccgc tcaacatcgg
     3421 tataaacatc gcaaacgtga gcaacccaag ctctggcaat tgaaatgcaa tgctcagaaa
     3481 agtattgtcg gtgaaaaaac gccccggcat tgccagacta aataacacca ccacaataaa
     3541 acatattgat aataagcctg aacgggtacc atctagtgga aaaaatatcc gcttgttcat
     3601 ttcgatcctc ccgttagacg aaaccaacat cagtttcttt acgctttttg taatgggtca
     3661 cacaaatcgc cgagacaata accaccccaa taacgatatt gacgaaataa ctcgagacac
     3721 caatcaaatt aaggccgttt ttaagaatac cgataagaaa tacgcccata agcgtaccaa
     3781 tgaccgtacc acgtccgcct gataaactgg caccgcccaa taccgctgca gccagcacat
     3841 ccaactctcc gccaaccaat gcattcggca caacttcact catacggtaa gcttgcagca
     3901 ttccaccaat tgctgcagcg gcaccaagat aaccgtaagc aaacagataa attagcgaca
     3961 cccgaatccc aatacgtctt gctgattcgg tattccctcc cacggcataa agctgacgcc
     4021 caatatgggt tttgttcatt atcacccacg tcagaaatgc cattcccagc ataaccacta
     4081 acggtaatcc gatctggtaa tcttctccgc caaattcaaa actcaggaaa gaacgctgcg
     4141 taatccacca atccggtaaa tcgtaaatac tgtgaccatt agtcaaccac atcaacatac
     4201 cgaacagtaa cgactgcata ctaatcgtaa tgatgatgga aacgacatta aggtaataaa
     4261 taagaatagc gtttatcaaa ccaaaaatga tgccaaaaaa aacagcaaac accaggcaga
     4321 aaaatggatt tgatagcgtt tcattaagca gtaacgacgc cacaacatat tgtacaaccg
     4381 aagctactgc tgcaaaagag atatcaattc cgccagtaac caatacgaca aataatccga
     4441 gggcgaaaat acccgttacg gcataacttt ccgagagatc gagcaagttc tgtattgata
     4501 aaaactcatc gctcatgatg gaaaaaaata ccagcatgag aacaatgacc catcctaacc
     4561 aagcttcgac tgaacgagga aaccgcagcc gcataaagct aaccattaat cacctccgcc
     4621 aactgctgct cggtataatt cgaaggcaac agctcatgaa taatctttcc gcgcttcata
     4681 tgaaaaatac gatcgcagtt ataataagct tctggtgctt catcagtaat taatatcact
     4741 gaaataccca cacctgaaag ccgatgaata agctggtaaa tactatcttt tgcgccaata
     4801 tctacaccta ccgttggcgc atccagaatt aaaaccttag gtcgtgttaa aatccattta
     4861 gccaggacaa ccttttgttg attgccacca gatagcgttg aaagtggatt atttggatcg
     4921 gtgaccttaa tatccaaatc ctggatccac tccttgacta aggtttcttt tcgattttca
     4981 tcaataaacc ccgatggcgt ctttaaacga tccagaatcg agagcaccat attatcgctg
     5041 atagattggg gtaatacggc ccccagtgtc aggcgatctt ctgaaacata tgctattccc
     5101 agttttaccg catgcgtgtt gtctttaatt gtaacgggct taccttcaag ataaatatca
     5161 ccactatctg ctcgagtaat accgaaaaga ctcaatgcta attcggttct ccccgcacca
     5221 agtaaaccac acaatcccag aacctcgccc tggtgtagtt ttagagatac atcttcaaat
     5281 tgcccttttc tgcataattt ttttagttca agaactactt taccttcaac gctatttggt
     5341 agttttttct ggtggactat agtttttccg gtcattaact cagtaatatc gcctggagaa
     5401 agttgattgg cagcccacgt tccaattatc tctccatcac ggatgaccgt aattcgatct
     5461 gagatttcct taacttcatc aaggcggtgg ctaacaaaaa ctacggtaat atttttatcg
     5521 cgtaagtaat tcactgtgcg tagtaattga ttaacttcag tgcgcgtcaa tgacgccgtt
     5581 ggctcgtcca tgatgactaa ccgggcatcg gcaatcagag cccggcaaat agcgacctgt
     5641 tggcgttgtg caatcggcaa atgttgtact aacgcatcag gatcgagatt gaattccagc
     5701 tcctgaatta ttttctgtga tacatcccga agttgttttt ttctgtgcag ccctaaatat
     5761 cccttcaggt tgtattcaaa agcgatattt tcagccacag aaagatttgg gaataaagaa
     5821 agatcctgat atatcacctg aatacccagt tcccgagctt tatcaggaga aagacgagtc
     5881 caggagttac catctataat aatttcactt cccgaatcag gaacataaac accacacagc
     5941 gttttgatca gagtactttt accacaccca ttcgcaccag caagacaatg aacctcacct
     6001 ttatttaagg taagattgat attttttaga gccctatggc caccgaaact tttcgttagt
     6061 gaccttagcg tcactaatgg ttgctccgca ttcttctcta taatatgagg catcataata
     6121 ttcctgaata cgactaaagg aattccgggt caaatttgac ccggtgattt acggtaaaat
     6181 tacaagccta acttcactaa atgtcgtgta ttttcaagat cgagcttttc tggattatca
     6241 ctataaatag tgttgttatt aaccttgatc tcgcccatat caccgatttt tacaccgtct
     6301 ttaataggtt caccgttcat cattgccgtc gcaacctgga caaaaacctt acccgcaatc
     6361 atagggtttg aaatatatcc cccatcaata gcgccttttt cgagtagctt aattccctga
     6421 cctggagtga aggttccaaa aacgcaaatt gcgtcatttt tcttccgctt atcaatagca
     6481 cgccccgcac caattggtcc ttgtgaacca aatgacataa tgcctttaag atctttatat
     6541 ttagacatta aatcgttcgt cgtgcgcatt gaatcatcca ccgattcagc aacaccaaaa
     6601 cgatcttcaa ccaacgtcat atttggataa tgttcttttt gataagcaat cgcggcattg
     6661 gcccagtcat ttaccaaagg cacggtcaga ctgccgacaa acatggcgta tttaccttct
     6721 tcgcccatgc acttcgccat atctttcata tgattggcac ccattgtctg ggtatttaac
     6781 aactcaaaat cccaatctgc atttttttga tctggtgatt cgtgggtaat aactttaata
     6841 ccggcttctt gtgcacgctt aagtacaggt tcaagaacct ttgcatcgtt aggaacaacc
     6901 ccaataacat ccacttttcg tgcaattaaa tcttcaatcg cccgaacctg ttcagctgga
     6961 tctgccgttg tagggccgac ctgccatgca tcaaccccca atagttttcc ttcatcttta
     7021 atgccttgtt ccatcacatt aaaccaggga atgccaccga cttttactac tacccccatc
     7081 gtaaaaggtt tattcgcttt ttgtgatgtt acagtctgat cagcccatgc agcagtggaa
     7141 gataatacgg tagcaataag gcagggtaag attaattttt tcatcgtaat ttccttaaga
     7201 aatagttcag gtgtcaggaa aa
//
LOCUS       DACBVI010000200         7173 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.201, whole genome shotgun sequence.
ACCESSION   DACBVI010000200 DACBVI010000000
VERSION     DACBVI010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.201"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            437..880
                     /locus_tag="IU325_20705"
     CDS             437..880
                     /locus_tag="IU325_20705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAP5811418.1"
                     /translation="MNSVFFSPGSKSFYLQELFPEYEDAGTLPDDVIEITRETYEQFL
                     GLHPEGKEIGADSSGRPIWINSPPPSKEDEVLMAEMKKISLVSEANTYINTHQWPGKA
                     AIGRLKGNELEQYNLWLDYLDALELVDPSSAPDIEWPTPPAVQAR"
     gene            complement(852..1454)
                     /locus_tag="IU325_20710"
     CDS             complement(852..1454)
                     /locus_tag="IU325_20710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAP5811419.1"
                     /translation="MHKAILNSELIATKAGDVTVYNYDGETQEYISTSNEYLAVGVGI
                     PACSCLDAPGTHKAGYAICRSADFNSWEYVPDHRGETVYSTKTGESKEITAPGDYPEN
                     TTTIAPLTPYDKWDGEKWVTDTEAQHSAAVDAAEAQRQSLIDAAMASISLIQLKLQAG
                     RKLTQAETTRLNAVLDYIDAVTATDTSTAPNVIWPELPEA"
     gene            complement(1454..3001)
                     /locus_tag="IU325_20715"
     CDS             complement(1454..3001)
                     /locus_tag="IU325_20715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP5811420.1"
                     /translation="MTVKYYAILTNQGAARLANATMLGSKLNLTQMAVGDANGVLPTP
                     DPAQTKLINQKRIAPLNLLSVDPNNQSQIIAEQIIPENEGGFWIREIGLYDDEGVLIA
                     VANCPETYKPQLQEGSGRTQTIRMILVVTNTEAITLKIDPSVVLATRKYVDDEVLELR
                     LYVDDQMRNHIAAQDPHTQYAQKHNPTFTGEPKAPTPAAGNNTSRIATTEFVQAAITA
                     LINGAPATLDTLKEIAAAINNDPKFSTTINNALALKAPLSSPALTGTPTAPTAAQSVN
                     NTQIATTAFVKSAIAAMVGSAPAALDTLNELAAALGNDPNFSTTVLNALAGKQPLDNT
                     LTNLSGKDVAGLLAYLGLGEGSALPVGVPVPWPSATPPTGWLKCNGAAFSAEEYPELA
                     KVYPKNKLPDLRGEFIRGWDDGRGIDTGRSILSIQGYATEDHAHGLPSRSTIVTDATI
                     NFYFDESWVNSGTDIIKRGNTNDAGLPAPDYGTFKTYKQSVAGLGAAASETRPRNIAF
                     NYIVRAA"
     gene            complement(2998..3609)
                     /locus_tag="IU325_20720"
     CDS             complement(2998..3609)
                     /locus_tag="IU325_20720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HAP5811421.1"
                     /translation="MSDSRLLPTGSSPLEVAAAKACAEIEKTPVSIRELWNPDTCPAN
                     LLPWLAWAFSVDRWDDKWPEATKRALIRDAYFIHCHKGTIGAIRRVVEPLGYLIEVRE
                     WWQLNEEPGTFRIVVGVLEQGITEEMYQELERLVADAKPASRHLTGLAISLSTTSNIF
                     AGAGCYHGDALTVYPYTPEAIIVGGDYFQASAIHLIDNLRVNA"
     gene            complement(3602..4510)
                     /locus_tag="IU325_20725"
     CDS             complement(3602..4510)
                     /locus_tag="IU325_20725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HAP5811422.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLI
                     ITPADDSTIPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELAAP
                     LADIVLNSTQASFCTEYRVVTGGSDE"
     gene            complement(4515..4862)
                     /locus_tag="IU325_20730"
     CDS             complement(4515..4862)
                     /locus_tag="IU325_20730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HAP5811423.1"
                     /translation="MTARYLGMNRSDGLTVTDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEPRVTLSSVTTARSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            complement(4859..5494)
                     /locus_tag="IU325_20735"
     CDS             complement(4859..5494)
                     /locus_tag="IU325_20735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HAP5811424.1"
                     /translation="MNTLANIQELARALRNMIRTGIIVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVMVKASTRITLDTPEVVCT
                     NRLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            complement(5561..6013)
                     /locus_tag="IU325_20740"
     CDS             complement(5561..6013)
                     /locus_tag="IU325_20740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HAP5811425.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSAELAKRLRQSQQRRVMA
                     QKAPDGTPYVPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEENRKDGKKIDYPARPLLGFTGEDVQMIEEIILAHLDR"
     gene            complement(6006..6473)
                     /locus_tag="IU325_20745"
     CDS             complement(6006..6473)
                     /locus_tag="IU325_20745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP5811426.1"
                     /translation="MLKPDSLRRALTDAVTVLKTNPDMLRIFVDNGSIASTLATSLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDEGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPTPPEPVTRPMELYINGELVSKWDE"
     gene            complement(6563..7006)
                     /locus_tag="IU325_20750"
     CDS             complement(6563..7006)
                     /locus_tag="IU325_20750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysB family phage lysis regulatory protein"
                     /protein_id="HAP5811427.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLAKHENASLRASLDRANNVASEQQ
                     TTITMLKNQLHVALTRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGADLPDAVRRLHQRPACTDASDCPQRLPESESLPDAGQRPGDER"
     gene            complement(6994..>7173)
                     /locus_tag="IU325_20755"
     CDS             complement(6994..>7173)
                     /locus_tag="IU325_20755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysA"
                     /protein_id="HAP5811428.1"
                     /translation="PVRWRRRAAALVVMVATFIALVAAGWIFTALIYLVASVFFRLIR
                     KACRQRFEGREPCQS"
BASE COUNT         1590 a         1960 c         1887 g         1736 t
ORIGIN      
        1 aacggaatgt gggcaacggg gaaaaccaaa tccctgatat gtctttctgg acggttactg
       61 gtggcaatgg aaattttgtg attcgtcaac ctgacgggct aatcactcag atggttactg
      121 taagtataag cggtccagtg gcgatgaatg gaatgactga taatgcttat gccattacag
      181 gttctaataa gtcttatatt gccacagcca cattgccctt tgtatttcct aataaggtgc
      241 tgggcgttat ccctctggta tcaacaacaa cctatggtgg tgtatccagt aatattacag
      301 gttcatacgc gacggcggtt tgttcttttg ccgctgtccg ggggaataat acgattgtat
      361 tcaaagtcga caaaccactg aatgcagcct ttccttcaga taccagcgtc tcagcgttaa
      421 tcattggacg gtaataatga actcagtatt cttttcaccc ggaagtaaaa gtttttatct
      481 acaagaattg tttccagaat atgaggatgc gggaacgctt ccagatgatg ttattgaaat
      541 tacccgagaa acatatgagc aatttcttgg gctgcatcca gaagggaaag aaattggcgc
      601 tgacagttca ggacggccaa tatggattaa ttccccaccg ccttcaaaag aggatgaggt
      661 gttgatggct gaaatgaaaa aaatatcttt ggtttcagaa gccaatacct acatcaacac
      721 ccatcagtgg cctggcaaag ctgctattgg tcgtctgaaa ggtaacgagc tggagcaata
      781 taatttatgg ctggattatc tggacgcact ggaactggtt gatccctcca gtgcgccaga
      841 tattgaatgg cctacgcctc cggcagttca ggccagatga cattcggcgc ggtgctggta
      901 tctgttgccg tcaccgcgtc aatgtaatcc agcacagcgt taagccgggt tgtttctgcc
      961 tgcgtcagtt tccgtccggc ctgtaatttc agctgaatca gactgatgga agccattgca
     1021 gcatcaatca gtgactggcg ctgtgcttct gccgcgtcta ctgcggcgct atgctgtgcc
     1081 tcagtatcgg tcacccattt ctcaccatcc catttatcgt atggcgttaa tggggcgata
     1141 gtggttgtat tttcagggta atcacccgga gcggtgattt cttttgattc tcctgttttg
     1201 gtgctataga ccgtttcacc gcgatggtct ggcacatatt cccatgagtt aaaatctgca
     1261 gaacggcaga ttgcataacc agccttatgt gtgccaggag catctaaaca ggaacatgcc
     1321 ggaatgccga caccgacggc aagatattca tttgaagtgg aaatatattc ctgtgtttca
     1381 ccatcatagt tataaacggt aacatcccct gcctttgttg caatgagctc actatttaat
     1441 attgctttat gcatcaggct gccctcacga tatagttaaa tgcaatatta cgcggacgcg
     1501 tttctgaggc tgcggcacct aaaccagcca ctgattgttt atatgtttta aaggttccat
     1561 aatccggtgc tggtaatccg gcatcgtttg tgtttcctct tttgataatg tcagtgccac
     1621 tatttaccca gctttcatca aaatagaaat taatagttgc atcagtcaca atcgtggatc
     1681 ttgacggtaa tccatgagca tgatcctccg ttgcatatcc ctgaatactt aaaatagagc
     1741 gacctgtatc aatcccccgc ccgtcatccc agccacgaat aaactcacca cgtaaatcag
     1801 gcaatttatt tttcgggtaa acttttgcca gttccgggta ttcttcagca gaaaaagctg
     1861 caccattgca tttcagccag cctgtcggcg gagtggcgga aggccacgga acaggcacac
     1921 caacaggtaa tgcagagcct tctcccaaac caaggtatgc gagaagacca gcaacatcct
     1981 ttccactcaa attagtaagc gtattgtcca gcggttgttt gcctgccagt gcattaagca
     2041 ctgttgttga aaagttaggg tcattcccca gcgccgccgc cagttcattc agtgtatcca
     2101 gtgccgcagg ggcagaaccc accattgccg caattgctga tttcacaaaa gccgtggtgg
     2161 caatctgtgt attgttgacc gactgtgccg cagtaggtgc tgttggcgtt ccggtaagtg
     2221 cgggactcga cagtggcgct ttcagtgcca gcgcattgtt aatggtggtg ctgaatttcg
     2281 ggtcattgtt aatggctgcg gcaatttctt tcagtgtgtc cagcgtggct ggcgcaccgt
     2341 taatcagagc ggtaatagcg gcctgaacaa actcagtggt cgcaatccgc gaggtgttat
     2401 ttcctgcggc aggcgtcggc gcttttggtt ctccggtaaa tgtcggatta tgtttctgcg
     2461 catactgggt atgaggatcc tgtgcggcaa tgtggtttct catctggtca tccacataca
     2521 gccttaattc caggacttca tcatccacgt atttacgggt cgccagtacc accgacgggt
     2581 cgattttcag cgtgatggct tcggtattcg tgacaaccag aatcatgcgg atagtctggg
     2641 tgcgtccgct gccttcctgc aactgcggtt tgtacgtttc cgggcagttt gccaccgcaa
     2701 tgagtacacc ttcatcatca taaagcccaa tctcacggat ccagaatcct ccctcgtttt
     2761 cagggatgat ttgctccgca ataatctggc tctgattgtt cgggtcaaca ctcagaagat
     2821 tcagcggtgc aatgcgtttc tggttaatca gttttgtctg tgccgggtct ggtgtcggca
     2881 agacaccatt cgcatcacca acggccattt gcgtcagatt cagcttactg ccgagcatcg
     2941 tcgcgttagc cagccgtgct gcgccctgat tagtcagaat ggcgtagtat ttcactgtca
     3001 tgcgtttact ctcaggttat caattaaatg aatggccgag gcctggaaat aatcccctcc
     3061 gacaataatg gcctccgggg tgtagggata aaccgtcagg gcgtcaccgt gatagcatcc
     3121 cgcaccggca aaaatgttgc tggttgtact taaactgata gccagtcccg tcagatggcg
     3181 gcttgccggt tttgcatcag caacgaggcg ctccagctcc tgatacattt cctcggtaat
     3241 accctgctca agcacgccaa caacgatgcg gaacgtcccc ggctcctcgt tgagctgcca
     3301 ccactccctc acctcaatca gatagccgag cggctccacc acacgccgga ttgcgcctat
     3361 agtgccctta tggcagtgaa tgaaatacgc atcgcggata agagcgcgtt ttgttgcttc
     3421 cggccactta tcatcccagc ggtcaacaga aaacgcccac gccagccacg gcagcagatt
     3481 tgccggacag gtatccgggt tccacagctc acgaatactg accggcgttt tttcaatttc
     3541 cgcacaggct tttgcggcgg caacttcaag cggtgatgag ccggtcggca gcaggcgcga
     3601 atcactcatc cgagcctccg gtcacgacgc ggtattcggt acagaaagac gcctgcgtgc
     3661 tgttaagcac gatatcggcc agcggtgcag ccagttcgac acgctgcacg ccttccacat
     3721 gcaaagcggc ataaatggca gacagacgga tgtcgcgccc aagccggtgc tgtgccgtga
     3781 tgtacgcttc cagttttttc acagcagcag cgcggatggg ttcgctttcg ggaccagggt
     3841 aaaggtaaag cgtggcgttt atctggtatt caacgatggc agcagactgc acggtcacgc
     3901 ggtcggccac cggcctgacg tcctcgccat taagggcgtt acgcaccacg gccagcaggt
     3961 cttcggatgc gacgccgtta ttttcacgtg acagcacgga gatagtgacg caggccggag
     4021 acggactggt gacagagata tccgcgacac gcccgtcagc actgcgacca tgatactgat
     4081 aggctcccac cgacccggcg acgcttaagc cctcaaacgc ctgctgaata cgcagacgat
     4141 aatcggtgtc agactccatc actgccggtg tcggcgggat agtcgaatca tctgccgggg
     4201 tgataatcag gcgcgtggtg ttgtaattgg caccaatcac atcaaggtca ttaccggcgg
     4261 cacaggccag cattaccgcc cgtgcggcct cattcacacg ctgacgccag ataagctcac
     4321 gataagcatt ttcctccagc agtttgacga gaggctcgga ttccagcgtc agggtacggg
     4381 caaccgcctc ctgctggtct tccgggtaaa gggaaatcag tgtcgccttg cgttcggcaa
     4441 gaatggtttc aaagtccagc tcctcgacca catccggtgc gggtagctgg ttcaggtcga
     4501 taatcggcat ggtttcaact cacagggatg gttaacgaaa gtggctggcc ggtgtcgttg
     4561 tgctgaccgg ttaacgtgac cgtcattcgc ccgtcaaaac tgcgcgccgt ggtgacggat
     4621 gacagggtga cgcggggttc ccatttcagc acggccatgt aacaggcgac cttaatctgc
     4681 aactcaagcg ccggggtctg cggctggtca atcattgacg ccagcaacga gccgtaatca
     4741 cgacgcatca cccgtgagcc gaccggcgtg cgcaggatat cgccgatact ctggctgata
     4801 tgctcaaggt cagtgacagt caggccatca ctgcgattca ttccgagata acgcgctgtc
     4861 atagaggact cccggttgtg ccgccgctgt cgccggggtg tttatgggta tgcagtacct
     4921 taccgtttga tgagagttca ccgccggtgt gttcaatgtt gccgcgcatc gtcccgccct
     4981 tctgcacttc cagcgtgccg gtaatcagcc tgttggtgca gaccacctcc ggtgtgtcca
     5041 gggtgatgcg ggttgacgct ttcaccatga ccaccggcac cgtggcagta acagaatcag
     5101 aagccgtcac gctggccgtt ttaattccgc ttaccgtgag tgcactggtt tcgggttcat
     5161 actcaatcac cgccccgtca gggaaacgga tatgcagggc atccgccgac gcagacggcg
     5221 cggggttatc gccggaataa atccccggca gaacgaacgc cgtgtcgagt tcaccgccca
     5281 cggccagaat cagcacctgt tcccccacgg aaggtgccca ccatgtacgc gaacgtccgg
     5341 cacgatgggt cagccactga agccagtcgg tgcacatgcc gccggtctgc acacggcagc
     5401 gaccggcgtt aaggtcggtt tcgacgataa tgccggtgcg gatcatgttg cgcagtgcgc
     5461 gcgcgagttc ctgaatattt gcgagagtgt tcataacggg aaggatgccg ccgggtcata
     5521 ccggcggcaa tgtgacgatg aggtgtcggg aatggcacaa ctaacggtcg aggtgagcca
     5581 gaataatctc ttcaatcatc tgcacatcct caccggtaaa gccgagcaga ggacgcgccg
     5641 gataatcaat tttcttaccg tctttccggt tttcttccga cagaccgaac tgatgcacgc
     5701 tggcgatttt cggcgacttc ccgccgtaaa attccattga tgcctgctcc gggctggcgc
     5761 ggatatgcaa aaaacgactg gtgataagtt tcgcaaacat ttttcgctta acgcgaccgg
     5821 tcttttttct ggcgctctgc tgctggcgtg gcacgtaggg tgtgccgtcc ggggctttct
     5881 gtgccatcac ccgacgctgc tgactctgcc gcagacgttt cgccagttcg gcactcagtc
     5941 gtcgacgccc tgacggtgac agcgactcaa tcagtccggt cagccggtct tcaaaacgct
     6001 taaactcatt catcccactt gctcaccagt tcgccattga tataaagctc catcgggcgg
     6061 gtgaccggct ccggcggcgt gggttccggg atattcttca catgcagcgc accgtcaacc
     6121 tcactgacca gcgtgcgctc ggtcagcatc aggctgatac tgatatcaaa gctgctgtca
     6181 ttgttgatgt ctgcataaaa cgtgaagccc tttttctggc cttcgtcggt agtcatgatg
     6241 tcgggctgat tttcccgcag ccacgccagc accggcacga tgagcaggtc aaaatcaccg
     6301 gtaaagtcgg tcacaatgac attgagcgtg taacgctttt cgaatgacag cgacgtcgcc
     6361 agtgtggagg caatactccc gttatccacg aatatccgca gcatatcggg gttagttttc
     6421 agcaccgtga cggcatcagt cagcgccctg cgcaggctgt cgggtttgag catcgttttc
     6481 gtcctgacag tatttaatca tttttacctg gctggcacag cgcgccagcg cgttctcaag
     6541 ctgccggata tcggcactta aatcaccgtt cgtctccggg tcgctgcccg gcatcgggca
     6601 aagattcact ttcgggcagg cgttgtggac aatcactggc atcggtgcag gcggggcgct
     6661 ggtgcaaccg gcgcacagca tcaggcaggt cagcgccgta ccagcggcga aaatcttcgt
     6721 tttcattgag taacctcgtg atggttttct cgcgctgtgc ttcacgtttc gcggcgttct
     6781 ccagctcctg acgcagtgcc acctgcgcca gctcgttttt gtctgccctg gtaagggcaa
     6841 catgaagctg gtttttcagc atggtgatgg tcgtctgctg ttcactggcg acgttgttcg
     6901 ccctgtccag cgaggcgcgc aggctggcgt tttcatgctt cgccagaaac agccccgcca
     6961 ccgccagcga taacaacacg accagcacaa tcatcagctt tgacatggtt cccgcccctc
     7021 aaaacgctga cggcaggctt tacgtatcag ccggaagaac accgacgcca caagataaat
     7081 cagcgcggta aaaatccacc cggcagcgac cagcgcgata aacgtcgcca ccatcaccac
     7141 cagagccgcc gcccgtctgc gccaccgcac cgg
//
LOCUS       DACBVI010000201         7167 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.202, whole genome shotgun sequence.
ACCESSION   DACBVI010000201 DACBVI010000000
VERSION     DACBVI010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.202"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            60..1109
                     /gene="allD"
                     /locus_tag="IU325_20760"
     CDS             60..1109
                     /gene="allD"
                     /locus_tag="IU325_20760"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HAP5811429.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            1131..2366
                     /gene="allC"
                     /locus_tag="IU325_20765"
     CDS             1131..2366
                     /gene="allC"
                     /locus_tag="IU325_20765"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HAP5811430.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VLGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            2377..3162
                     /gene="allE"
                     /locus_tag="IU325_20770"
     CDS             2377..3162
                     /gene="allE"
                     /locus_tag="IU325_20770"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HAP5811431.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(3291..4436)
                     /gene="glxK"
                     /locus_tag="IU325_20775"
     CDS             complement(3291..4436)
                     /gene="glxK"
                     /locus_tag="IU325_20775"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HAP5811432.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(4458..5759)
                     /locus_tag="IU325_20780"
     CDS             complement(4458..5759)
                     /locus_tag="IU325_20780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HAP5811433.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(5816..7162)
                     /gene="allB"
                     /locus_tag="IU325_20785"
     CDS             complement(5816..7162)
                     /gene="allB"
                     /locus_tag="IU325_20785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HAP5811434.1"
                     /translation="MIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVMDASGL
                     VVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDRASIEL
                     KFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDNDFRDVN
                     DWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKSEGRVTAHDYVASRPVFTEVEA
                     IRRVLYLAKVAGCRLHICHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTDQFEEI
                     GTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGGIAGLQ
                     NCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQPNSSY
                     VLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILKHQQ"
BASE COUNT         1850 a         1921 c         1771 g         1625 t
ORIGIN      
        1 tattttccct gtatgttaat tgcagtcatg cttcacaccg tcgttaaaaa ggaagacaga
       61 tgaaaatcag tcgggaaaca ctccaccagc taattgagaa taaactctgc caggctgggt
      121 taaaacgtga gcacgctgca accgtggctg aagtattggt ttacgccgat gccagaggga
      181 tccactctca tggcgcggtg cgcgtggaat actacgcgga acgcatttca aaaggcggca
      241 ccaaccgcga accggagttt cgtcttgagg aaaccgggcc gtgctcggca attttacatg
      301 ccgacaatgc cgccggacag gtcgcggcga aaatgggtat ggaacatgcc atcaaaaccg
      361 cccagcaaaa tggcgttgcg gtggtcggta tcagccggat gggtcacagc ggcgcaatct
      421 cttattttgt gcagcaggca gcccgcgccg gattaattgg catttcgatg tgccagtccg
      481 atccaatggt ggtgccgttt ggcggcgcgg aaatttacta cggtactaac ccactggcct
      541 ttgccgcgcc gggagaaggc gacgagatcc ttacctttga tatggcgact accgtacagg
      601 catggggaaa agtgctcgac gcccgctcgc gtaatatgtc tatcccggat acctgggcgg
      661 tcgataaaaa cggtgcacca acaaccgatc cgttcgcggt acatgctctg ctccccgccg
      721 ccgggccgaa agggtatggc ctgatgatga tgattgacgt cctctcaggc gtcttactcg
      781 gcttaccgtt cgggcgacag gttagttcga tgtatgacga tttacacgcc gggcgtaatt
      841 tggggcaatt acatatcgtt attaacccga actttttctc ctccagcgaa ctattccgtc
      901 aacatcttag ccagaccatg cgcgaattaa atgccataac ccccgcgccc ggttttaacc
      961 aagtttatta tcccggacag gatcaggata ttaaacaacg caaagccgcc gtcgaaggca
     1021 tcgaaattgt tgatgatatt taccagtatt taatttccga cgcgctttat aacacgtcat
     1081 acgaaacgaa aaatcccttt gcgcaataat tattgagaca ggactttctt atgattacac
     1141 atttccgtca agccatagaa gaaacgctgc cctggctttc ctcttttggc gctgacccaa
     1201 cgggtggtat gacccgttta ctttattcgc cggaatggct ggaaacccag cagcaattta
     1261 aaaaaagaat ggcagcaagc gggctggaaa cacgtttcga tgaagtggga aatttatacg
     1321 gtcgcctgag tggcaccgaa tatccacagg aagtggttct gagcggttcg catatcgata
     1381 ccgtggttaa cggcggtaac ctcgacgggc aattcggcgc gctggcggcg tggctggcaa
     1441 ttgactggtt gaaaacgcaa tacggcgcgc cgttacgtac ggtcgaagtg gtggcgatgg
     1501 cagaagaaga aggcagccgc ttcccgtatg tcttctgggg cagtaaaaat atctttgggc
     1561 tggcgaatcc tgacgacgtg cggaatatct gtgatgccaa aggaaatagt tttgtcgatg
     1621 cgatgaaggc ttgtggattt actctgccga acgccccact aactccgcgt caggatatta
     1681 aagcctttgt cgaactgcat atcgaacagg gctgtgtgct ggaaagtaat gggcagtcaa
     1741 ttggcgtggt gaatgcaatt gtcgggcagc gtcgttatac ggtgacgctg aacggcgaat
     1801 caaaccatgc aggcaccacg ccgatgggtt atcgtcgtga tacggtttac gctttcagtc
     1861 gcatttgcca tcagtctgtc gaaaaagcga aaaaaatggg cgatccgctg gttctgacct
     1921 ttggcaaagt agagccgcgc ccgaatacgg tgaatgtggt gctgggtaaa accacgttca
     1981 ccattgattg tcgtcatacc gatgctgccg tgctgcgcga tttcacccaa cagttagaaa
     2041 acgacatgcg ggcgatttgc gatgaaatgg acattggtat tgatatcgat ttatggatgg
     2101 acgaagaacc cgtgccgatg aacaaggaac tggtcgccac cctgacagaa ttgtgtgaaa
     2161 gagaaaaact gaattaccgg gtgatgcaca gtggtgccgg gcacgacgcg caaattttcg
     2221 cgcctcgcgt accaacctgc atgattttta tccccagcat caacgggatc agccataacc
     2281 cggcggaacg caccaatatt accgaccttg ccgaaggggt caaaacgttg gcactcatgc
     2341 tttatcaact tgcctggcag aaataaggag tcataaatgg gatatttaaa taacgtcacc
     2401 ggttaccgcg atgatttact ggctaaccgt gcgattgtta aacacggtaa tttcgcactg
     2461 ttaacaccag acggtctggt aaaaaatatt attccgggct ttgaaaattg tgacgcgaca
     2521 atcctctcca cgccaaagct gggtgcctct tttgttgatt atctggtcac actgcatcaa
     2581 aacggtggca accaacaggg cttcgggggc gaaggcattg aaacgttcct gtatgtgatc
     2641 tctggaaata tcactgccaa agccgaaggt aaaacatttg ccttaagcga aggcggctat
     2701 ctttattgcc caccaggctc cttaatgacg tttgttaacg cccaggccga agacagccaa
     2761 atctttttat ataagcgtcg ctatgttccg gtagaaggtc atgcgccgtg gctggtttct
     2821 ggcaatgcca gcgaactcga acgcattcat tatgaaggca tggacgatgt tattctgctg
     2881 gattttctgc ccaaagagtt aggttttgat atgaacatgc atatcctctc ttttgcgcca
     2941 ggtgccagcc acggttatat cgaaactcac gtccaggaac acggtgccta tattctttcc
     3001 ggtcaggggg tttataacct cgacaataac tggatcccgg tgaaaaaagg cgattacatc
     3061 tttatgggcg cttattcctt acaggccggt tatggcgtag ggcgtggtga agcgtttagt
     3121 tatatttact caaaagattg caaccgcgac gtagaaattt aagtcatacc actcgcctga
     3181 ttatttttag ctatgttgga ttttgccacg gtttataccg tggctttttt tgcggtaaat
     3241 tcaggtaaaa acggatgcgg gataaaatgc atccgctcta atggcaagag tcagttttta
     3301 attccctgac ctattttaat ggcgcaggca atatttcgtg cgctgttgaa gagattggtt
     3361 tcaccgctgg cgagcacttc ggctaaaggt gccagacgtg gcaaaatgct gaacaccgcg
     3421 tcaatgccgt actggtgcac cacttccacg ccatcaccca atacgccagc aatcccaatc
     3481 accgggacat taaactgctt cgccaccgac gccacgccca gcggcgcttt accacctgcc
     3541 gtttgcgaat cgatgcgccc ttccccggtg atcaccagcg ctgcaccctg caccgcctgc
     3601 tccaggttga ccgcccgcag tacaatctca atacccggtt taatatccgc attgaggaat
     3661 accgccgccg caatacccat accgcccgcc gcgccgccgc ccgccatctg acagacgttg
     3721 atttcagttt gctgttgtaa aacacgggcg taattttgca gcccctgctc cagctcttcg
     3781 accatttccg gcgttgcccc tttttgcggg ccgaacaccg ccgccgcgcc gcgtgcccct
     3841 accagcggat tatcaacatc acatgcgact tcaatatggc agttcgccag acgcggatcg
     3901 cattcatcca tctcaatgct tgccacgcgc gctaaattgc cgccgttagc tgccagcacc
     3961 tgaccgtcgg catcgagaaa acgcacacca agcgcctgcg ccatgcccat accgccgtcg
     4021 accgtcgcac tgccgcctat gccgagaata atatggcgaa tgccgttatc cagcgcatga
     4081 cgaattaact cccccgtacc aaaactggag gccagcaacg gattacgctt ttcaggcgcg
     4141 accagcatca gaccacttgc agccgccatc tcaatcaccg ctgttttccc gtcgccggta
     4201 aggccataaa aagcattcac tttttcgccc atcggcccgc agacttcaag cgtcacgatg
     4261 ttgccgcccg tcgcggcgac catcgcatcc accgtccctt cgccgccatc cgctatcggc
     4321 aaacagatat agttcgcatc gggaaagagg gtcgaaaacc cggctttaat tgcctgacaa
     4381 catttttctg cacttaagct ctctttaaac gagtctggcg caatgacaat cttcattatc
     4441 aatccttttt caacaaatta ttcgatgcgt tcccacggaa taagattatc cataagaaca
     4501 gccagtaaaa tcccgaccag taagccgtta ctgagcagag gacgaagcgt taatggaagg
     4561 tcttgcagat acacaggcgg taatgccatt aaaaatatgc cgacaaataa cggcaatgcg
     4621 agtcgataaa tattgcgagc ggtaaaggtt atttgctggc taaacaccag cgcggaaaag
     4681 agtaaaggca gataagaaac cagcatgacc gcgctactca cgggtaaagg gatactgcaa
     4741 aacagtcgtg tgagtgccgg aaccagcgcc accagcaggc aaataacgct gccataaata
     4801 aacgaacgcc gcgtgtaatc gccagtctgg gttaataaac caatggatga aacaaacggt
     4861 gaaaatggaa ttaccgccag cggtacggtt atcagcgtca taaatccggt cgccacaaag
     4921 ctacgacgat aacgcgtatt ccccgcgccc tgctgcggat aaaaaacatc cgtgccccga
     4981 atcgcaccgt aggtattgct gatatttacc agacctgtaa tcaccgccgt cagaataatc
     5041 cccggcgaca aagcacctcc actgcccagc gggaaccact gccagtgcaa ctcaccggag
     5101 agcgaatgcg aagaaggaaa gcaaaagtac cacaacaacc agccggttat cgtaccgacc
     5161 agcaggccat aacgggcaaa acgttgcggc agaaagataa tcatcgccag taccaggcac
     5221 atcaccgcga ccgaaagcgc gaacggcggt aactgaattt taaaattcgg gtcggctatg
     5281 ccaaacggca gcccgagcat acctttgaaa aagatagtgg tcagctgcgc gcccagcatc
     5341 aacataaaca agaccatcac cgacggcgta aataaccgcg ccagacgatg acctaatccg
     5401 ctaaaaccaa tcaacatcgt cagcacgcca gagagcgcaa ttcccaccgc caggctggta
     5461 gcgatatcgt tgatcggtgt cccgcgcgat gcttcgccaa gggtgatcgt aaggatggtt
     5521 ccccaccaca ggccacccgg cccttccata atagcgcgac gatgaccgca gaacgcctga
     5581 gcgaagcagg ccagtgcggt agcaagaaaa gcatattgcg tgagcgtaag caggctgctt
     5641 tgcggcaact gaaaagcaga aagtagcgta ggaggaacca caacagtgtt gcaaaaaata
     5701 aagaaaaacc actgaaatcc tgataacagg ctttcgcggc tgactgcaaa attgaacata
     5761 aacacacctt aaccggagaa tgcccgccgc aaggacgggc attgcagggg ccagattact
     5821 gctgatgttt aaggataaat tgacctttcg gcgcaacagg gaagccctgt tcaatgtcgt
     5881 aaatcacatc accacgtaag atggttttcg tgatacgcgc gccaatggta cggccaacat
     5941 acgggctgac tttgtggcga tattccagat cgtcattggt aagaacatag ctgctattag
     6001 gctgaatgaa gacgaagtcg gcatcttttc ctggggcgat acggcctttt tgctgcagac
     6061 cgaaaatatc tgctgcgtta gtcgccatta atttgccgaa cattggcaga gacattccgc
     6121 gtttctgtac cgcttcatcg aacatcacgt ccatacagtt ttgcagaccg gcaataccgc
     6181 cccatgcttc catgatgttg ccggctttca tttccggcgg gcatggtgag tggtcagaaa
     6241 ccaggcagtc gatttcaccg ttaaatagtt tttcccacat gcctttctgg ttttccagat
     6301 cgcggatcgg cggtgaacac ttcgccagag taccgatttc ttcgaactga tcggtatcca
     6361 gtacaaagta atgcgggcag gattcacagg taacatcctg accttcctga cgtgcacgag
     6421 tcacttcttc aacaccttcc gggctgctga tatggcaaat gtgcagacgg caaccggcaa
     6481 ctttcgccag gtacagtacg cggcgaatcg cttccacttc ggtaaatacc ggacgcgaag
     6541 ccacatagtc atgggcagtt acgcgacctt cacttttcgc ttcttcgccc agtgcatcac
     6601 agatcagtgc gttttcgcag tgcaccagca ccggctgccc cagttcgccc agcttctgcg
     6661 cacctttgaa gaactgccag tcattgacgt cacggaagtc gttgtcgata ccgcgatcgc
     6721 cacaggtcgc aacgaagcat ttgaagccga caacgcccac ttcatccagc tcatgcagac
     6781 ggtcgatgtt gtaagacacc aggccaccga gttgcgccgc atcgatagtc agcttgcctt
     6841 tagcagcatc gaacttcagt tcaattgaag cgcggtcaac cgttgcaggc agctggttga
     6901 gcggcatttc gatcatggtg gtgataccac cttttgccgc tgcgcgagta ccggtttcat
     6961 aaccttccca gtggctacga cccggttcag aaatatgggt gtgcgcatca accatgcccg
     7021 gcgaaaccac cagaccagac gcatccatca cttcttttgc atcgcccaga tcctgaccga
     7081 tagcagcaat ttttccgcct ttaacggcga tatctacaac gcgagcttcg ttttctaaaa
     7141 taacagtgcc gtttttaatg attaaat
//
LOCUS       DACBVI010000202         6978 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.203, whole genome shotgun sequence.
ACCESSION   DACBVI010000202 DACBVI010000000
VERSION     DACBVI010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.203"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..687)
                     /locus_tag="IU325_20790"
     CDS             complement(<1..687)
                     /locus_tag="IU325_20790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP5811435.1"
                     /translation="MRLLLSLDMKILRSSYLKVRGFLIKGCIVVLNYNLCGVMICLHR
                     KGYMRLIVKTVTGLTKVRHRNEVGVTLASLSLSAKRVLFLALCQIDTKEMLDDDILEV
                     DADFFSKATSLDKYASYAALKEGAKVLSSTTLVLNRDDLKNLADELGILSSKNKIPDR
                     LDLNLTEFCAYYDHLATVRIKFTNTAKRYFSKLIGSENRYTTQVLKSVVLLNSVNSTN
                     LYQVIRKYYSQ"
     gene            1298..1450
                     /locus_tag="IU325_20795"
                     /pseudo
     CDS             1298..1450
                     /locus_tag="IU325_20795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(1628..1786)
                     /locus_tag="IU325_20800"
     CDS             complement(1628..1786)
                     /locus_tag="IU325_20800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811436.1"
                     /translation="MRAITPLTPARPISDYQTMFSLSQLARELANGTPGQCETLARGA
                     VSARYEMR"
     gene            2040..2555
                     /locus_tag="IU325_20805"
     CDS             2040..2555
                     /locus_tag="IU325_20805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HAP5811437.1"
                     /translation="MNIQEALNVFGLSGDLTEKDIKAAYKKAALKYHPDRNPLGAELM
                     KAVNAAFDFLMANIDKINQFQSTDENARYNYGEDLEKVLNTLSGLTGIVYEVIGNWVW
                     ISGETKEHKDILKEMGCKWASKKKQWFYRPEEHKSRWNRKEHSIEEIREMYGTAGKRK
                     ASGWTRVEASA"
     gene            2614..2850
                     /locus_tag="IU325_20810"
     CDS             2614..2850
                     /locus_tag="IU325_20810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811438.1"
                     /translation="MKNKTITEAELINIFESYGAYICPDEIEVTAKECNENGSVLHRG
                     LNAEGWAHLFAKEEAYQQECEAQEAASDDGHFDE"
     gene            2910..3461
                     /locus_tag="IU325_20815"
     CDS             2910..3461
                     /locus_tag="IU325_20815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811439.1"
                     /translation="MKEQKNFFERYKPVFEIVCRILGNGWRVNLLDDCQYRIKLTSPD
                     FKNYSIHIRMEKGRLVIIGSVDSRSWRSPYHTCTVSPERNPVEIAADIEKKILSDALD
                     NVDMAREYEQQLQQKREKKLILKGMLSRLVHLESWHGTLTGFKVENGLDGNVSERGDG
                     YEMVIRGLSVDQLIKVAGFIKQL"
     gene            3527..3739
                     /locus_tag="IU325_20820"
     CDS             3527..3739
                     /locus_tag="IU325_20820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811440.1"
                     /translation="MSEDATRLFIELEKLMKVRSKEADKKTTKKIVFQKSFYYEEFSN
                     VKDEIDDPILLKFYTDTIVKVQNHEF"
     gene            3729..3971
                     /locus_tag="IU325_20825"
     CDS             3729..3971
                     /locus_tag="IU325_20825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811441.1"
                     /translation="MSFKPTPEEVKQARIKAGFTQQEAAERFGFTLSAWQAKETSGKT
                     SRGLAAGTYELLLLLADEHPDYQLVKREKNQDKVTK"
     gene            4065..4403
                     /locus_tag="IU325_20830"
     CDS             4065..4403
                     /locus_tag="IU325_20830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811442.1"
                     /translation="MNTYVITFQALNTKKEKITCSAFVHSETLFLAVHSFEDKNRGRG
                     YVITSVSELLSEELTAKNSLKKNINFWFNECGLSKSEVINEVINWKNFAYTLKELEEA
                     KNEAIRELRS"
     gene            4639..4896
                     /locus_tag="IU325_20835"
     CDS             4639..4896
                     /locus_tag="IU325_20835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811443.1"
                     /translation="MKKQEFLDFISAEQRRGAVRFSLGFNSKGEIVLHWTNEAGLRVW
                     SILSGNRGKSPSRANRERMSNLRRWLHDARQGMEGDTPEAE"
     gene            5237..5782
                     /locus_tag="IU325_20840"
     CDS             5237..5782
                     /locus_tag="IU325_20840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 1"
                     /protein_id="HAP5811444.1"
                     /translation="MKKVQFRIEESQHDDLLDCLKTLYPDEPGLTVAKGMKLLANALL
                     KSKVKNEDVNVSKDNDDFIKTTMYLTGKQRTLIEKAAHRHGWNLSRECRYRIQTTLEN
                     ELDFFDQELLVMNRCRNAIDKIGRNFHYIIVNDNARVLDKDGFYQDAERLSSEIMSLK
                     SEFENYIMLCKGRTVSNKVEV"
     gene            5785..6945
                     /locus_tag="IU325_20845"
     CDS             5785..6945
                     /locus_tag="IU325_20845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP5811445.1"
                     /translation="MGVYVDKEFRVKRKSSEAGRKSAFAHKVKNGGKNYQRNVQERIN
                     RKGASKEVVVKISGGAITRQGVRNSIDYMSRESELPVMSESGQVWKGDEIQEAKEHMI
                     DRANDPQNVFDDKGKENKKVTQNIVFSPPVSAKVKPEDLLESVRKTMNKKYPNHRFVL
                     GYHNDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKMKGYDVKATHKQQ
                     HGLNQSIKDAHKTAPKRQKGVYEVVDVGYDHYQNDKTKPKQHFIKLKTLNKGVEKTYW
                     GADFGELTTRENVKKGDLVKLKKLGQKEVKIPALDKNGVQHGWKTAHRNEWQLENLGV
                     KGIDRISSSSKELVLNSAEMIKKQQLQMRNFSQIKQSMIQSEQKVKIGIRLG"
BASE COUNT         2216 a         1372 c         1590 g         1800 t
ORIGIN      
        1 ctgactgtaa tatttcctaa tgacttgata aaggtttgtt gagtttacac tgttcaacaa
       61 cacaactgat ttcaatacct gagtggtata tctattttca gaaccaatca acttagaaaa
      121 atatcgctta gctgtatttg tgaattttat ccgtacagtt gcaagatgat cgtaataggc
      181 acagaattct gttaagttta aatccaagcg atcaggtatt ttattcttag atgatagtat
      241 cccaagttca tcagccaaat tttttaaatc atctctgttt aaaactaacg tagttgaaga
      301 aagaacttta gctccctctt tcagagctgc ataagaggca tatttatcta aagaagtagc
      361 ttttgaaaaa aagtcagcat caacctccaa aatatcatca tctaacattt cctttgtatc
      421 aatctggcaa agagccagaa aaagcactct ttttgctgaa agggaaaggg atgcaagagt
      481 taccccaact tcatttctat gtctaacttt cgttaatccc gttactgtct tcacgataag
      541 cctcatataa cctttcctat gtaggcaaat cataacccca caaaggttgt aattcaagac
      601 aacaatacaa cctttaatta aaaaacctct cacctttaga tatgaagatc tgaggatctt
      661 catatctaaa gatagcaaca accgcatgtt tcacttgatg ttcagtcaaa ctgtggaaac
      721 cgttaaaaat cttctcacct tacgtgaaaa atcctctcat ctttagtgaa aaatctaact
      781 accttaactt aaaaatcttc tcaccttacg tgaaaaatcc tctcacctta cgtgaaaaat
      841 cctctcacct ttagcaattt ttttccataa ttttcaggta attgaagcga gctaagtttt
      901 taagttttta agatcaagaa gaaaatataa ggcttttaat gcacagcgtc tgtggataac
      961 tcagcataaa tccagaggat agatccgtct ggcctccctg agcactgcac caccaaaaat
     1021 tgagcaaata ttaaccttag cgcgtaagtg ctctattgaa accccgtgga aattccgctt
     1081 cctcgctcac tcgcgggctt cgccctgtcg ttcggctgcg gcgagcggtg taatctctct
     1141 aaaagatcca gacgggatcc ttttccgatc caattcttaa atgctctcaa aagccctcag
     1201 aggcgtttta accgctttaa cccggtcatg gttcaacgga gcgggtaaaa tgcgcttaga
     1261 acgtcataca gctcgttttt aacctgatcc ctaatcaggg ggtataacag aggcggccaa
     1321 aaaatccggc cttgctcgtc agagtatcta ccgcgcatta tccccgaacg gaaacccgac
     1381 catcacaaca cttgcacagc ttgcagcagt agccgggtta cagttcactc tcagtaagcc
     1441 caaccactaa cctttcagac aagcccctca taccgggggg attatttctt atgccagcaa
     1501 aatttcacga ccggcgagga tggccaagtg ttttttgctg gcaattttcg tgcagcagct
     1561 ggcgcccgga ttcatttttt gctgtcaggc atcaatcggt ttcagcaacc cgccccgtaa
     1621 ttcgctgcta acgcatctca taacgcgcag atacagcgcc ccgcgccagt gtttcacact
     1681 gacccggcgt accgttcgca agctcacgcg ctaactgcga caggctaaac atcgtctgat
     1741 agtcagaaat tggcctggca ggggtgaggg gggttatcgc tctcattcgg gcttgttggt
     1801 tcgccagtcc tggcgaacgt ctccggcgcg atgttacgac agcgcattat gtaactacag
     1861 ttcgcgcctc tttcggaaat cccttcatgc gcggtaaagc aatatacatc tttatgtaat
     1921 ctttacaatg taaagcatta aaaatgattc cataaccatc atttgatggt ataatgaatt
     1981 tgttggttgg gaatggttcc ctgccagatt ccagaagccc acacgggcgg gagcgaaaaa
     2041 tgaatattca ggaagcatta aacgtttttg gtttatccgg tgatctgact gaaaaggaca
     2101 tcaaggcggc atacaaaaaa gcggctttaa aatatcatcc agaccgcaat ccattgggtg
     2161 ccgagctgat gaaagcagta aatgctgcgt ttgattttct catggctaac attgataaaa
     2221 tcaatcagtt tcaaagcact gatgaaaacg cacgttacaa ctacggtgaa gatctggaaa
     2281 aagttctgaa tacgctttcc ggccttacag ggattgtcta tgaagttatt ggtaattggg
     2341 tctggattag cggagaaact aaagaacaca aagacatttt aaaggaaatg ggctgtaagt
     2401 gggcatctaa gaaaaaacaa tggttttacc gtcctgaaga acacaaaagc cgctggaacc
     2461 gtaaagaaca cagcattgaa gaaattcgtg aaatgtatgg tacagcgggt aaacgtaagg
     2521 cgtcaggctg gacacgtgta gaagcaagcg cataaccgga atgggggcga aagcccccta
     2581 gaacatggca aagattgatt cggagacacg ttgatgaaaa acaagaccat aacggaagcg
     2641 gagctgataa acatttttga aagttacgga gcctacattt gcccggatga gatcgaagtt
     2701 acagccaaag aatgcaatga aaatggttca gtcttgcacc gtggcttaaa cgcagaagga
     2761 tgggcgcatc tttttgcgaa ggaagaagca tatcagcagg aatgcgaagc ccaggaagct
     2821 gcaagtgatg atggacactt tgatgaatag tcttaaaact gccgtaattc gatttacggc
     2881 ttttttattg agttcaaaag gctgaatcga tgaaagaaca gaaaaatttc tttgaacggt
     2941 ataaaccggt gtttgaaatc gtttgtcgaa tcctggggaa cggctggcgt gtaaatctgc
     3001 tcgatgattg ccagtacagg attaagctaa cctcacctga ttttaaaaat tactctatac
     3061 atattcgaat ggagaaagga aggttagtta tcattggaag cgtagatagt cgcagctgga
     3121 gaagtcctta ccatacttgc acagtatcgc cagagcgtaa ccctgtagaa atcgccgccg
     3181 atattgaaaa gaaaatcctg tcggatgctt tagataatgt agatatggcg cgagagtatg
     3241 aacaacagtt acagcaaaaa cgcgagaaaa aactgatcct gaaaggaatg ctatctcgtt
     3301 tagtgcatct tgaaagctgg catgggacat tgaccgggtt taaagttgag aatgggttag
     3361 atggtaacgt atcagaacgg ggcgacggat atgaaatggt aattcgtggc ctgagtgtag
     3421 atcagctcat aaaggtagcg gggtttatta aacagctatg aggcaaaaaa tgaatgaagt
     3481 cctgagcgac aggaaaaaca aaggtaatgt aacatacagg attgttgtgt ctgaagatgc
     3541 aacacgtttg tttattgaac ttgaaaaact gatgaaggta cgctctaaag aagcggataa
     3601 aaaaacaact aaaaagattg ttttccaaaa atcattttat tacgaagaat tctcgaacgt
     3661 taaagatgaa attgatgatc caattttatt gaaattctat acagatacta ttgtgaaggt
     3721 acaaaatcat gagttttaag ccaacacctg aagaggtgaa acaagcacgt attaaagctg
     3781 gctttacgca gcaggaagcc gctgaacggt ttggttttac gttatcagcc tggcaagcca
     3841 aagaaacatc cggtaaaacg tctcgtggtt tagccgctgg tacgtatgaa ttacttttgc
     3901 tcctggctga tgaacatcca gattaccagc tcgttaagag agaaaaaaat caggataaag
     3961 tgactaaata aattgcatta ccatcatatg atggtataat ttatttgtag gtcgggaata
     4021 gttccttacc atgatccagt agcccaaacg ggcgggagac taaaatgaac acgtatgtta
     4081 tcactttcca ggctttaaac actaagaaag aaaaaatcac ctgctctgct tttgtccatt
     4141 cagaaactct cttccttgcc gttcattctt tcgaagataa aaatcgcggt cgtggttatg
     4201 ttatcacgtc tgttagtgaa ttgttgtccg aggaactgac ggcgaaaaat tccctcaaga
     4261 aaaatattaa cttttggttt aatgagtgtg gtttaagtaa atctgaggtt ataaacgaag
     4321 taattaactg gaagaatttc gcatataccc ttaaagaact ggaggaagcc aaaaatgaag
     4381 cgataagaga actgcggagt taatcaatta ggggtggtat caggttgtgg aggcaacctg
     4441 ataccgtgac actcaattcc ctcgcgagga atataaaatg tcggaacgca tgttatcagc
     4501 aatacagact gttgaaaagg gtgggcgtcc ggtttttccc ttgatgccat tctctgcttt
     4561 tcctgagtac atggcattac tcagaaaagc cctggaaaag aaagaaacaa aagcactgat
     4621 agaaaaacag gaggtgctat gaaaaaacag gagtttttag attttatcag tgcagaacag
     4681 aggcggggag ctgtccggtt ttcgctggga tttaacagca aaggggaaat tgtattgcac
     4741 tggactaacg aggccgggtt aagagtctgg agtatactaa gcggtaacag gggaaaaagt
     4801 cccagccggg caaaccggga aagaatgagt aacctccgcc gctggctcca tgatgcccgg
     4861 cagggcatgg aaggcgacac accagaggca gaataaaatt cccgttcctg aatcgccgaa
     4921 gccctcccgt ttacgggagg tgcttttttt gtatattccg taaaacagcg ctcgcgcccc
     4981 ctgccaggcc attcgcgtag cgaatttatt tccgcccaag ttagccgcga atccgttgtc
     5041 tccggtcgca gacgcaccag atcacagcca cgatagcctt tcattttttg catttcgatg
     5101 gacgctgaag cacaatttat gtgtggaggt agttcactgc gatgcaggat agggcaaacg
     5161 ccgcaaaatg acgtctctga cgccattccg caacgtttgc ccggtgacct acagtcggtt
     5221 cttgttggag agttttatga aaaaagttca attcaggatt gaagaaagcc aacatgatga
     5281 tctacttgat tgtcttaaaa cactttatcc agatgaaccg ggtttaaccg tggcaaaagg
     5341 aatgaagctg cttgccaatg ctttactgaa aagcaaggtt aaaaatgaag atgttaatgt
     5401 gtcaaaagac aatgatgatt ttatcaaaac cacaatgtat ttgacaggga aacaaagaac
     5461 tttaatagag aaagcggctc atcgtcatgg ctggaattta tccagagaat gtcgttatcg
     5521 aatccagacg acacttgaaa atgaacttga ttttttcgat caggaacttc tggtgatgaa
     5581 tcgttgccgt aatgcaatag ataaaatagg gcgaaacttt cattatatta tcgttaatga
     5641 taatgccaga gtacttgata aagatgggtt ctatcaggac gctgagcggt tgagttcaga
     5701 gataatgagt ctcaaaagtg agtttgaaaa ctacattatg ttatgcaaag ggcgaactgt
     5761 ttctaacaag gttgaggtgt aatcatgggc gtttatgttg acaaggaatt cagggttaag
     5821 cgtaagtcct ctgaagctgg ccgcaagtct gctttcgccc ataaagttaa aaatggtggt
     5881 aaaaactatc aacgcaacgt tcaggaacgt atcaaccgca agggtgccag taaggaggtt
     5941 gttgtcaaaa tctcaggagg tgcgattacc cggcagggag ttcgaaacag tattgactat
     6001 atgagccggg agtcagagct gccagtgatg agtgaaagcg gtcaggtatg gaaaggtgat
     6061 gagattcagg aggctaaaga gcacatgata gatcgtgcta atgaccctca aaatgttttc
     6121 gatgataaag gtaaggaaaa taaaaaagta acccaaaata ttgtgttttc accgcctgta
     6181 tcagcaaagg taaaacctga agatctgctt gagtctgtca ggaaaacgat gaacaagaag
     6241 tacccaaatc atcgttttgt gcttggctat cataacgaca aaaaagaaca tccacacgtg
     6301 catgtcgttt ttcgtattcg tgataatgac ggtaaacgag ctgatatcag gaaaaaagat
     6361 ttacgggaaa ttcgtacagg tttttgtgaa gagctgaaga tgaagggcta tgacgttaaa
     6421 gccactcata agcagcagca tggacttaac cagtctatta aagatgcgca caaaacagcg
     6481 ccaaaacgac agaagggcgt ttatgaggtt gttgatgttg gctacgacca ttatcagaac
     6541 gataaaacca aaccaaagca gcatttcata aagcttaaga cactgaacaa gggcgtggag
     6601 aaaacatatt ggggggctga ttttggtgag ttaaccactc gcgagaacgt taagaaaggt
     6661 gatcttgtta agctgaagaa gttaggacaa aaggaggtga aaatccctgc acttgataag
     6721 aacggtgtac agcatggctg gaagactgca cacaggaatg agtggcagct agagaacctg
     6781 ggggttaagg gcatagacag aatctcttca tccagtaaag agctggtgct gaacagcgcg
     6841 gagatgatta agaaacagca actccagatg agaaatttct cgcaaataaa acagtcgatg
     6901 attcaaagcg aacagaaagt gaaaatcgga attcgtttag gataattcat actctccggt
     6961 aacttattga ttaagatc
//
LOCUS       DACBVI010000203         6788 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.204, whole genome shotgun sequence.
ACCESSION   DACBVI010000203 DACBVI010000000
VERSION     DACBVI010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.204"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(375..2630)
                     /locus_tag="IU325_20850"
     CDS             complement(375..2630)
                     /locus_tag="IU325_20850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HAP5811446.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKAFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASLLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELSDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWTHQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCHIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNQGGAAGY
                     IAKYISKNIDGYALDGQLDNDTGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSTSDE
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWTDPEVVRALRGALKHGLRRPSRQ
                     QRNGSALKPHEIAPSARLTRSERMQITSIRVDLAQNGIWTQRWELEALARGATVIYDE
                     KRYIYPVADEW"
     gene            complement(2620..2895)
                     /locus_tag="IU325_20855"
     CDS             complement(2620..2895)
                     /locus_tag="IU325_20855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811447.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGSYGR"
     gene            complement(2892..3116)
                     /locus_tag="IU325_20860"
     CDS             complement(2892..3116)
                     /locus_tag="IU325_20860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HAP5811448.1"
                     /translation="MPDNVDFIQEQQAELLERQINAARVKHCGASALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRMA"
     gene            complement(3116..3418)
                     /locus_tag="IU325_20865"
     CDS             complement(3116..3418)
                     /locus_tag="IU325_20865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811449.1"
                     /translation="MSCSHSVVLLNNALKIAVMKNGDLSLIQLGLDKEKREITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTEIASYCADEFKKLNDKRSW"
     gene            complement(3418..3642)
                     /locus_tag="IU325_20870"
     CDS             complement(3418..3642)
                     /locus_tag="IU325_20870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HAP5811450.1"
                     /translation="MHTVSESQCGKYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(3706..4206)
                     /locus_tag="IU325_20875"
     CDS             complement(3706..4206)
                     /locus_tag="IU325_20875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HAP5811451.1"
                     /translation="MTVMTLNLVEKQPAAMRRIIGKHLAAPRWQETCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHAGLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(4384..4659)
                     /locus_tag="IU325_20880"
     CDS             complement(4384..4659)
                     /locus_tag="IU325_20880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811452.1"
                     /translation="MNRQLVSVTDAVPYQEFAKLIGKTPRAVRGMIEKGKLPVIEITD
                     PQSVSGRAGEYWVYLPAWNNGLKLAYESRPKEIRDGWLMWLGLGEPR"
     gene            4781..5080
                     /locus_tag="IU325_20885"
     CDS             4781..5080
                     /locus_tag="IU325_20885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP5811453.1"
                     /translation="MSIDVSEKLKLIRESERLNRKEFSELTGVAYSSLSSYESRSKNA
                     GVEAIMKVLQHPRFTKYTLWFMTDQVAPEAGQIAPALAHFGQNETTSPHSGQKTG"
     gene            5196..6209
                     /locus_tag="IU325_20890"
     CDS             5196..6209
                     /locus_tag="IU325_20890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP5811454.1"
                     /translation="MAIKKLDDGRYEVDIRPTGRNGKRIRRKFDKKSEAVAFEKYTLY
                     NHHNKEWLSKPTDKRRLSELTQIWWDLKGKHEEHGKSNLGKIEIFTKITNDPCAFQIT
                     KSLISQYCATRRSQGIKPSSINRDLTCISGMFTALIEAELFFGEHPIRGTKRLKEEKP
                     ETGYLTQEEIALLLAALDGDNKKIAILCLSTGARWGEAARLKAENIIHNRVTFVKTKT
                     NKPRTVPISEAVAKMIADNKRGFLFPDADYPRFRRTMKAIKPDLPTGQATHALRHSFA
                     THFMINGGSIITLQRILGHTRIEQTMVYAHFAPEYLQDAISLNPLRGGTEAESVHTVS
                     TVE"
     gene            complement(6486..6785)
                     /locus_tag="IU325_20895"
     CDS             complement(6486..6785)
                     /locus_tag="IU325_20895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811455.1"
                     /translation="MSLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDNQYYTEA
                     EASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFKCGE"
BASE COUNT         1841 a         1691 c         1494 g         1762 t
ORIGIN      
        1 ttaaatagtg gaaactggct atcgcaagtg cggaatgagg ttaattatac tcattcaatg
       61 ggcgcttggt ttccttatac tggagcaagt tataactatg acattatgta tcgaaaaata
      121 accacatgga gaggcaatct ggattcaatc atgtttagcg acgatcataa cgatgaagtt
      181 atgtttgccg aaacatgtgc agcaattgtt gctatgtgca aaggactatt agaagagcta
      241 tataatgcca accccaaatg ttttctctca tatggagcta aaaaatgttt aaactactgt
      301 aatgggcatg ataataacac tatattttaa ttaccatatg aattaaaaag ataattacaa
      361 aaagtaatca acttctacca ctcatcagca accggatata tgtatctttt ctcatcatat
      421 attacggttg ccccacgcgc cagcgcctca agctcccacc gctgagtcca gataccgttc
      481 tgagcaaggt caacacggat gctggtgatt tgcattcgtt ccgatcgagt cagtctggcc
      541 gatggtgcaa tttcgtgtgg ttttaacgcg cttccgtttc tttgttgacg gcttggtctt
      601 ctcaagccgt gttttaatgc acctctgagc gccctcacga cctccgggtc agtccattcg
      661 ataacaccgt catctaccag attaagcact gctgcggcgt gttcagaagg cgtgggagcc
      721 ggtaacgaag tatcaccacc ggtgagcttt ccacagttat tgacaggact ccgaggcgcg
      781 gcgatgccgc tttttaaagt caaaggctca acgaccggaa ctttcggcac aatgcgccag
      841 tccgtcgttc tggtgatatg aatatgacgc gcgccgagat gcggcgcgta aatgccgacc
      901 actctctcga cttcttcctc gtactcgtta acttcatccg acgtactacg ggcgaccctg
      961 acagtctgac aatcgcgcgg gacatttgcc ccaccctgcg cgctgatata caacgcaaaa
     1021 tcaccactgt ctgcggcggc gcgtgcagcc tcgacgcgct cgtcaaactc atcagcaatg
     1081 ctgacgccgc gaggcaattt gcgtagttca cggtaagccc ccattgtcgg cagaccaacc
     1141 gttttaaatt gtgggatgcg ccacgttgac gcccatgcgg taacagccgc agcagtgtct
     1201 ttcagcggtc tgccggtatc gttatcgagc tgaccatcca gtgcatagcc atcgatgttt
     1261 tttgagatgt atttcgcgat ataccccgca gcaccgccct ggttaaggtg ttttgcctga
     1321 aaacggtttc gcgcggctcc tctttcgtcg ccatcctctt tgagcgcata gcgacgcatg
     1381 atttcgataa tctggttacg ctggcgtgga ttacaaaaaa gcatcatatg ccagtgcggc
     1441 gttccgtcgt ggtgtggctc gacgacacgc aaaccgtaga cctgtaaatc attatccttg
     1501 aatgccgtgc gcatcaggct ccagatatgg cagagataac gctgcgcatc ctttggatta
     1561 aatgcctcat cgttccagcc gtgatttagc tggacggttt tactttcgcc ttttccgacc
     1621 tgacgtgtcg ggtgatactt tgacggcgcg gtaagcgtga taaacatccc cacatcaccc
     1681 tctgcggcgg cgtaacgctc aataccggca atggtgttca tcagctccat ccggcgaatt
     1741 tcaggattag aaatactgcc catcacctta ctgataaggt cgatgcgctc gccggtttcc
     1801 ctgttttcaa ggtcacacga tttaagaaat tccagatttg cctggcggcg tgcacgcaca
     1861 tcacgaatgg catgtttact ggcataagga gaacggtctt tattcacctc cccgacagca
     1921 atcagtaacg cctcatgcca gcgcatacgc tggcctttga gctgatgagt ccaccactca
     1981 tcgttaaaca gacgggcaat ggcagaatat gcctgcctcg tggtcatctg tcctttacgg
     2041 tattttttcc agtaaagcgg ggaaatattg aaagcacgtg cagcgccagc aacatgacca
     2101 tagaggtgag cctgcgcctc atccgtaaac agcgattctt tttcgccatg tgcatccacc
     2161 caggcatcgc tgagttcctc atacatcatg aaaagctgcg atgagatacg ggcggcaaac
     2221 tttttcagct ccttgtcatt cattcccggc aggcgcgcat agtggtcacg ctctgccaga
     2281 aacagcaacg acgcgtcggt gttcatttca tggcgctgat tcacgcgctc aatgcgcggc
     2341 cataaacgac gctgaaaagt ggatgtgagg aaataaaacc cgtgcaccgg gcttttattg
     2401 cgccggatgt agtcatagcg tgaagtaaac agcgagcgca aaaagtaagg caggcggtta
     2461 atcgtggata aaacaccttg cacctgacgc atctcgtcac gtgtaagggg tctttcgcgc
     2521 ccgacagcct cgcgtggcgc gttccatgca taagcaccgg taaacgcctt accggtgcct
     2581 gcggcaaatg ctgacggagg gacaaaacgc ccggaggctt taacggccat atgagccaaa
     2641 agcctctgaa caacgcttgc tgagttgctc aacctgcgcg tttaaatcag caaaagactt
     2701 tgcgcttccg gtcagaatat cgtgatgcat caggccggaa acgagctggc ttaatttcgg
     2761 gtaataacca accaccgcca gccattcctg accggcgttt ttaccgcttt ccgctctctt
     2821 tttctcgtgg agaataaact gaaagctgtc actggtaacg acataacgtt cgccaatttc
     2881 gatacgaata ctcatgccat tctccggtaa tgtttgtttt ttgcttcaaa gactgactga
     2941 caggaaacac aacgcgtggc tgacggataa gccgcacgac gggcagcagg tattggcgcg
     3001 tcacactctt cgcaaaccag cgcagaagca ccgcaatgtt ttacccttgc cgcgttaatc
     3061 tgacgctcca gtaattcagc ctgttgttcc tgaataaaat ctacgttgtc cggcattacc
     3121 agctcctttt gtcgttaagt tttttaaatt catcagcgca atagctggca atttctgtcg
     3181 ttaatttcgt cagttcatcc acggaggaga tttgcttgtg aaatacagcg cgtttaacaa
     3241 gtaaattgac cacatcagac aggagattta attcgttctg ataaatcgcg ataacagact
     3301 cagttatttc gcgtttttct ttatcaagac caagttgaat aagagataaa tcgccatttt
     3361 tcataacagc gatttttaag gcgttattca gtaatacaac tgaatgagaa caggacatca
     3421 aagcacctcc ccgcgagata atccgatatt gtgaaatttt tccgactcct gactgagcag
     3481 ctcgactatc tccacgcggg ataactccgc ctttgtgatg tggcgaatca tggcgtcaag
     3541 atgagaagaa aagcgcgtcg ctgcgtcggc ctgtgcttcg gttctggcct gttgcagcag
     3601 taatgcgtat ttaccgcact gactttcaga aactgtatgc atgactttct ccaggcaaaa
     3661 agaagtcccg cacaattaag tgcgttaaaa actctggtta attacttaat gcagatattg
     3721 ctctggtttt accgacgtca gaattgtcgg tgcatactca aacaggctga ataattcacg
     3781 taatgcacgg aataaagcat cacgccagta acatgattct tcattaattc gccagtatgg
     3841 ctggttaaat tctttttcag tcaatccggc atgcataaat aaagtacgac gctgactgac
     3901 tgttaaaaaa ctaatatatg catactcact tgcgccaacc tgacggcgtt ttgagaatgc
     3961 cccacgcaat tcatcaattg cacaaaccag ccgttcacgt tcgacgtcgt tcatttcttc
     4021 aaaacgcatc gttgcgtgac gctgttttaa ctgcgcatgg aagcaaaccg ttagccgttc
     4081 gcgttccatc atctgattat aataatcgca tgtctcctgc cagcgagggg cggccagatg
     4141 cttaccaatt atccggcgca tagctgctgg ctgtttttca acgagattga gcgtcatcac
     4201 tgtcatttcc agaccctccg gcttttcaga aaggtcagag ccttttttaa cggactctgt
     4261 tttttggtgc ggataatgat tcccttacgc cccttaccgt gggtgatggt gaagtcaatc
     4321 gccctggggc tttcgttacg caataactga gcaatacaac gaggctcatt catacggttc
     4381 tccttaacgt ggttcaccga gacctaacca catcaaccag ccgtcacgaa tctctttagg
     4441 acggctttca taagccagtt ttagtccgtt attccatgcc ggaaggtata cccaatattc
     4501 accagcacgc cccgatactg actgagggtc agtaatctca ataactggta atttcccttt
     4561 ctcaatcatg ccccttacag ctcttggagt tttaccaatg agttttgcaa actcctgata
     4621 aggcacggca tcagtcacgc ttacaagctg tctattcatc tgctacgatt ctcccttagt
     4681 gcttctaatg gctcctaatg gctaattatt gcctaaaagg ataactccag aagcacaata
     4741 tttcacacta tcagcaagaa attacgcaat cggagtaatt atgtcaatag acgtttcgga
     4801 gaagttgaag ctaatccgtg aatctgaaag gttaaaccgt aaagaattca gtgaattaac
     4861 tggtgtagcc tacagctcac tttcgagcta tgagagccgg tcaaaaaacg ctggagttga
     4921 agccataatg aaggtcttac aacatcccag atttactaaa tatactttgt ggttcatgac
     4981 tgatcaggta gctccagaag ccgggcaaat tgcgcccgct ctcgcacact ttgggcaaaa
     5041 cgaaacaacg tcgccccact ccggtcaaaa gactggttaa caatttatcg tgaatatatt
     5101 catcacaagt gcctactatt ggtggctaaa tttcagccac cacgaaaaaa gcgattagta
     5161 gtagcaaaaa aaagtaccac tcggagggtt ttctgatggc aatcaaaaaa ctcgatgatg
     5221 gtcgatatga agtggacatc cgccctactg gacgtaacgg aaaacgcatc cgtaggaagt
     5281 ttgataagaa aagcgaagct gtcgctttcg aaaaatacac gttgtacaac caccacaata
     5341 aagaatggct atcaaaacca acagacaaac gacgtctgtc ggaactgaca cagatctggt
     5401 gggatttaaa gggtaaacac gaagagcatg ggaaatctaa tcttggaaaa attgaaatct
     5461 tcacaaaaat aacgaatgac ccatgcgcat ttcaaatcac gaaatccctt atcagccagt
     5521 actgcgccac ccgaagaagt cagggtatta aaccttcgag tatcaatcgt gatttaacat
     5581 gtattagcgg catgtttaca gccctgattg aagcggagtt attctttggt gagcacccta
     5641 tcagagggac aaagaggctt aaggaggaaa aaccagaaac aggctatctc acacaggaag
     5701 aaattgcctt actgcttgca gcacttgacg gcgacaataa aaagattgcg attctttgcc
     5761 tgagtacagg agcacgttgg ggagaagcag ctcgtttgaa agcagaaaat atcatccata
     5821 accgcgtcac gtttgttaaa acgaaaacaa acaaaccacg caccgtcccg atctcagagg
     5881 ctgttgccaa aatgatcgcg gataacaaac gaggtttttt attccctgat gctgattacc
     5941 ctcgcttcag acgaacaatg aaagcaataa aaccggattt gccaacgggg caagccacac
     6001 atgcactaag gcacagcttt gccactcatt tcatgattaa tggaggaagt attatcacac
     6061 tacaacggat actaggtcac acgcggattg agcaaactat ggtttacgct cattttgcgc
     6121 cagagtacct tcaggacgcc atttctctta atccgctaag aggtggtact gaagccgaga
     6181 gtgtccacac agtgtccaca gtagagtaac gtttaagggc tttcagtggt aatttatgcc
     6241 gctcaaaccc gcattgtacc gttgaaagcc cctactggtg acaccctaaa tctcccttac
     6301 acgggcttat tttttgtgca taaaccatag agacggattg atcccaggtg accgtcttcc
     6361 attgaccaca tcgatagaat ctcccttcat agcacgatgc ctttcacgta acggcatcgt
     6421 gctcgcacag gttccggcta agcacaacca gaacgcgcat gtttgacgct taccaaaaaa
     6481 tattctcact ctccacattt gaatgtcaga cgagcgacgc catgtaatcc tgcaccttct
     6541 gtcttcaggt caactatctg catttttttg ccctgagtaa cacagaaatg agctgcatca
     6601 ttttttacta tattttctgc accagatatt ctgcccctgg ctaaagaagc ttcggcttcg
     6661 gtgtagtatt ggttatcgag tttacgctga atattacttt tatatgcaag accaaattta
     6721 ccgatacttg tctcatcatt atgtacagca cacccagaca taagaaaaac actaattaat
     6781 gatatagc
//
LOCUS       DACBVI010000204         6737 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.205, whole genome shotgun sequence.
ACCESSION   DACBVI010000204 DACBVI010000000
VERSION     DACBVI010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.205"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(98..583)
                     /gene="sixA"
                     /locus_tag="IU325_20900"
     CDS             complement(98..583)
                     /gene="sixA"
                     /locus_tag="IU325_20900"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HAP5811456.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(786..2930)
                     /gene="fadJ"
                     /locus_tag="IU325_20905"
     CDS             complement(786..2930)
                     /gene="fadJ"
                     /locus_tag="IU325_20905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HAP5811457.1"
                     /translation="MEMASAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIANCKTAQEAEALARQGQQLMAEIHALPV
                     PVIAAIHGACLGGGLELALACHGRMCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPQSILLEAAVELAKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDRQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTAIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGTQGQGRLSAPQVAERCVM
                     LMLNEAVRCLDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMSKRGESFWKTTATDLQ"
     gene            complement(2930..4240)
                     /gene="fadI"
                     /locus_tag="IU325_20910"
     CDS             complement(2930..4240)
                     /gene="fadI"
                     /locus_tag="IU325_20910"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HAP5811458.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAIANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVVEAE"
     gene            complement(4421..4705)
                     /locus_tag="IU325_20915"
     CDS             complement(4421..4705)
                     /locus_tag="IU325_20915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 family protein"
                     /protein_id="HAP5811459.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5077..6417
                     /gene="fadL"
                     /locus_tag="IU325_20920"
     CDS             5077..6417
                     /gene="fadL"
                     /locus_tag="IU325_20920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HAP5811460.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVDITGHSDLTGQSANAKNIAPT
                     AWVPNLHFVAPINDQFGWGASITSNYGLATEFTDNYAAGIYGGKTDLQTINLNLSGAY
                     RLNNSWSFGLGFDAVYAKAKIERYAGSLGPMLSQQLIARGVPASLTNGINTPDSQIAH
                     LKGDEWGFGWNAGILYELDKDNRWGLTYRSEVKIDFEGDYKSSINPNLNNILGSMGLP
                     YGTMGATQNGSLTLNLPEMWEISGYNRVAPQWAIHYSMAYTSWSQFQELKAKGDSGQT
                     LFYKDEGFRDAYRIALGTTYYYDKNWTFRTGIAYDDSPVPADKRSISIPDQDRLWLSA
                     GLTYAFNEDASIDVGASYMHGQKVKFTEGEGPAAYSFESEGKAWLFGTNFNYAF"
BASE COUNT         1676 a         1798 c         1593 g         1670 t
ORIGIN      
        1 cggatcattg ttagtacaat aagattgcat aaattcaaca ttgccagaaa aaaataaagg
       61 cccgatagat tacaccagcg ggccttgtgc atgcgtatta aatcgctttt gccatcttca
      121 gattacacgg gctcatctgc cagttgaatg tgccgtttcc gctttcatca agcgttacgc
      181 tggcaatggc cgaggtggta aacataggcg gcgtttcgcc cggacataac tcagcaacca
      241 gatacccgac taagggtaag tgggagatca ccagtaccga agcaacacct tcattggtca
      301 acgcctgtaa ataggcactg accagaccaa catcgccgca gggcgttaac tccggcagaa
      361 cttctgcact ggaaggcagg ttcagacaat cccctacttc ttccagtgtt tgctcggctc
      421 gcaggaacgg gctcaccaga acgcgttcga tttccacttt ttgacctttc agccagttcg
      481 ccatcaggcg agattcgtca caaccgttag tagtcagagg acgaaccgaa tcactggcgg
      541 catcgagggc tgcgtcgccg tgacgcatga taaaaacttg catattgcac cgcttttgtt
      601 aaccagattc gcccgttttc tttcatctga aaccagagaa agaaaacggt ggccggcatt
      661 gtgccttatc cattcaccga atgaaacgct gttttttacc tcaatggcgt aagtatagtc
      721 aatatgcgtt tacattttga gcaatactcc gccattcagc gcggattcat atagctttga
      781 ccttcttatt gcaggtcagt tgcagttgtt ttccaaaaac tttccccacg cttgctcatc
      841 tcgactaaac gctcgcaagg ggtaaaacgg gaaccatact gcgtggccag tcgttgcatt
      901 attgcaacca cttcacccgc accgagagaa tcgatgtagc ggaacggacc accgagaaat
      961 ggcggaaaac caatgccaaa taccgcgcca atatctccgt cacgcacgct acggataacc
     1021 tgctcgtcca gacaacgtac tgcttcattc agcatcaaca tcacacaccg ttcagctacc
     1081 tgcggtgcgg agagtcgccc ctgcccttgt gtgccaatca gcgggtaaat ggcgggatcg
     1141 acctgttttt tgcttttacg ccctttctga ccataaagat agaaaccccg gccatttttt
     1201 ctgcctttgc gatcgtcgtt caaaattgaa gaaacaacat ttgcaggcgc gctaaaacgt
     1261 tctccataag cggcttccag tacaggaata attttagtcc cggtgtcgat tcctacctca
     1321 tccaaaagtt ggattgggcc taccggaaaa ccaaatttca ccagcgcggc atcaatgtgc
     1381 tctacccgtt caccttcggt caacatgcgg atagcttcat taatgtaagg cgctaaaatg
     1441 cgattgacgt aaaacccggc tttgtcacgc acgacaattg ccgttttacc ctgttttttc
     1501 gccagtttta ctgtggtagc gatggtttgc gccgatgtcc ccgcatgagg aataatctcc
     1561 accagcggca ttttttccac cggactgaag aaatgcaggc caataacttg ctcaggtcgc
     1621 gtagcgtgag cggcgatatc accaattggt aaagatgacg tattcgaagc aaagatggta
     1681 tgagcggcgc aattttgctc aacttccgcc accatttgtt gttttaattc gagattttca
     1741 aacaccgctt caataatcag atcgcgatgg gcaaagccgc gatagtccgt cgttccggag
     1801 attaatgcca gctgtctgtc acgttcgctg gctttgagat gacgacggcg aactttgccc
     1861 tccagctgat cccaactgta cttcagcgca tgatttatgc cctgcgggtt aatatcttta
     1921 attctgaccg gaagccccgc tttacaagca gtgacgtaag caataccgcc gcccatcaag
     1981 ccaccaccta aaattcccac gctgtttaat ggcgcaggcg gcgcatcact gccggggtct
     2041 ttcttcactt ccgtactggc aaaaaagata ctacgcagcg cctgcgattg cggcgtcatc
     2101 gccagttcgc caaaagcacg ggcttcagcg tcatagccgc tactggtgcc ttgcgctaat
     2161 cccgtttcga caacctccag gatgcgttct gtcgccggat agttaccctg agttttgtgt
     2221 tctgttttct tgccgaccat tttgaacagc agcgcacgac ctaacggccc cgccagaata
     2281 cgctcgcgta caggtagagg gcgtgaagat ggacgatcct gctttgccag ctcaacagcg
     2341 gcttccagta aaatggattg cggaacgacg tcatccacca gccccagctt taatgcctgt
     2401 ttcgcccgaa gttgttttcc ggtgaggatc atctctaatg ctgtgctgac gcctatcaga
     2461 cgcggtaaac gctgggtgcc gcctgaaccg ggtaataatc caagttgtac ttcaggcaaa
     2521 ccgagcaccg ttttaggatc gtcagtacac atgcgaccgt ggcacgccag cgccaactcc
     2581 agcccaccgc ccaggcaagc gccatgaata gccgcgataa ccggaacggg caaagcatga
     2641 atctccgcca ttaactgttg accctgccgc gccagtgctt ccgcttcttg cgccgttttg
     2701 cagttggcga tcatgttgat gtctgcgcca gcaatgaagt tgtctggttt cgcggagata
     2761 aacaccacgc cgcgcaactc tttgttttca cggagttgct taataatggc gcgcacctgc
     2821 gaggcaaact ccgccttcag ggtattcatt ttctcacccg gtacgtcgat ggtgataaca
     2881 gcaatgttgt ccagacggac attaagggta aacgctgatg ccatttccat tattccgcct
     2941 ccacaaccat tgccgcgcca agcccacccg cagcacaggc ggtcactaat ccaaatccac
     3001 cgccgcgacg ccgaagttca tgcaatgtct gggtaatcat ccgcgcgccg gtcgccgcga
     3061 agggatgccc gtaagcaatc gaaccgccaa gcacgttaaa tttgctatcg tccacttcgc
     3121 cagtggcatg tgcacgcccc agtacatcac gagcaaaacg ttcgctaccc agcaactgaa
     3181 tattcgccag tgtctgagct gcaaaggctt cgtgcatatc gatcaatgtc agatcgctca
     3241 ttgtcaaacc ggcacgttcc agcgccagcg gcgttgacca ggccggaccg agcaacatgt
     3301 cctgccagac atctatcgca gtaaatgcgt aactgcgcag ataccccagc ggtaccagcc
     3361 ctaattcttt cgcccgcgat tcggtcatca ggatcaccgc cgctgcgcca tcggtcagcg
     3421 gcgtactgtt tgctgccgtt accgtcccgt gtttacgatc aaacgccgga cgcaactttg
     3481 cgtaatcagc aagcgaggaa ttaccgcgaa tattgttgtc ttcgacaagc ggttgtttat
     3541 aaggagggat aaaggcagtc atcacctctt ctttgagttt accgtctgac cacgcctgag
     3601 cggctcgctg atgcgaacgg tgcgctaatg catcttgctg ttcgcgagta ataccgtagg
     3661 ttttcgccat ttgttcagcg gtgtcgccca tccgcaagcc agtagagtat tccgccactg
     3721 ccggaggcac cggcattaag tcgcgcaaac gcaggcgaga gaagagtttc aggcgctggc
     3781 tcatggttcg agctttgttg acatcaacca gcacgcgcgc cagtttttta ctgacgccaa
     3841 ttggcaatac ggaagaggaa tcggccccac cggcaatccc cgcgcgaata gttcccgcca
     3901 ttaggctttc tgcgacgttt gcaatcgcct ggaaactggt agcgcaagcg cggctgacgc
     3961 tgtaagcatc ggtatgcaca ttcattccag taccgagaac aatttcacgc gcaatgttgg
     4021 gggcttccgg catttgtacg acctgaccaa aaaccagttg ttcaatcact tcggcgggga
     4081 tctcgctgcg tgccagcaat tcgcctacca ccatcttccc taaatcaacc gcgggaatgc
     4141 catgaaaagc cgtcgcctgg cgggcaaaag gcgtacgtaa accgctaaca atggcgatac
     4201 gatcgccctg gcgggtaacc agcggtaaaa cctgacccat aacactcccc tgtaaaaaaa
     4261 taaataaagt ggtctgacct gatcatagtc ttaaccattt ttttacattt agccaagcgg
     4321 agaaaaggga aagtgggagc tatgacacag agagaaaagt agaagagaaa ataaaacgcc
     4381 cctgccaaca actgacaggg gcgtacgtac ccgagaggaa ttaacgcaga cccagctgga
     4441 agatcagcat ttcagcttcg caggcaaaag tgaaatcgat atccaggcgc acaccgtcag
     4501 actcttcagt gaaagtcggg gtgattttgc acggttcgga ttccacgcta cgggcttttt
     4561 cagtcagcgc cgccagcgtt tgttctgctt ctgcgcggtt tgcaaacacg cggctgtaag
     4621 acgcggtgca gtcggagttg tccataatgg tgccaacatc catacagcag caaaccgggg
     4681 tttcatcagc actacattta ctcatcgttg atttcctctg tatgtgcacc caaggtgcca
     4741 gataaacgtt gtggatattt tacgcttccg gaaagtgctg ctccagttgt taattctgca
     4801 aaatcggata agtgaccgaa atcacactta aaaatgatct aaaacaaaat tcacccgaat
     4861 ccatgagtgc gccacctcca aattttgcca gctggatcgc gtttcttaga tcatatttga
     4921 aaaaagatag aaacatactt gcaacattcc agctggtccg acctatactc tcgccactgg
     4981 tctgatttct aagatgtacc tcagacccta cacttcgcgc tcctgttaca gcacgtaaca
     5041 tagtttgtat aaaaataaat cattgaggtt atggtcatga gccagaaaac cctgtttaca
     5101 aagtctgctc tcgcagtcgc agtggcactt atctccaccc aggcctggtc ggcaggcttt
     5161 cagttaaacg aattttcttc ctctggcctg ggccgggctt attcagggga aggtgcaatt
     5221 gccgatgatg caggtaacgt tagccgtaac cccgcattga ttaccatgtt tgaccgcccg
     5281 acattttctg cgggtgcggt ttatattgat ccggatgtag atattaccgg acattctgat
     5341 ttaaccggtc agagtgcaaa tgctaaaaat atcgcaccga cagcgtgggt acctaacctg
     5401 cactttgtcg cgcctatcaa cgatcagttt ggttggggag cgtcaataac ttccaactac
     5461 ggcctggcta cggaatttac cgacaactac gctgctggta tctatggcgg taaaaccgat
     5521 ctgcagacaa tcaaccttaa ccttagcggg gcttatcgcc ttaacaatag ctggagtttt
     5581 ggtcttggtt ttgatgcggt ttacgccaaa gcaaaaattg agcgttacgc cggttcgctg
     5641 gggcctatgt tgtctcagca gttgattgcc cggggtgtac ccgcttcatt aaccaatggt
     5701 attaatactc cggactctca aattgcccat ctgaaaggtg acgaatgggg cttcggctgg
     5761 aacgcgggta ttctttatga actggataaa gataaccgtt ggggcctgac atatcgttca
     5821 gaagttaaaa tcgacttcga aggggattac aaaagttcca ttaacccaaa tctgaacaat
     5881 attttgggta gcatgggctt accgtatggc acgatgggtg caacacaaaa tggctccctg
     5941 acgctcaacc tgcctgagat gtgggagatt tctggttata accgtgttgc gccgcagtgg
     6001 gcaattcact acagcatggc ctataccagt tggagccagt tccaggagtt gaaagcgaaa
     6061 ggcgatagtg gccagactct gttctataaa gatgaaggat tccgtgacgc atatcgtatc
     6121 gcgttgggca caacctacta ttacgataaa aactggacct tccgtaccgg tatcgcctat
     6181 gacgatagcc cagttccggc agataaacgt tctatttcca ttcctgacca ggatcgcttg
     6241 tggttaagtg ctgggttaac ctacgcattt aatgaagatg catccattga cgttggcgca
     6301 tcttatatgc atggtcagaa agtgaagttt actgaaggtg aaggaccggc agcttattcc
     6361 ttcgagtctg aaggtaaagc ctggctgttc ggtactaact ttaactacgc gttctgataa
     6421 cgcgttcgcc tggataaagt cacctgctat gcaggtgact ttaactcccc cacttcaccg
     6481 aaagtagttt cctcattgct tgcctcctga gttttgcaaa cagcctgatg gcagcttgcc
     6541 ctttcaaatc agtaagtggt atccacagac aaacctgaag ggaaacgcat attttcaggc
     6601 gttctgctcg tccttctcaa agagtttact tttctgcatt tccaggatac tcaacctccc
     6661 tggctattgt gcgctcatac actcaaatta aagataggct ctaaataaat gagcgttttt
     6721 tgatagtcta ttttatt
//
LOCUS       DACBVI010000205         6662 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.206, whole genome shotgun sequence.
ACCESSION   DACBVI010000205 DACBVI010000000
VERSION     DACBVI010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.206"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..562
                     /locus_tag="IU325_20925"
     CDS             <1..562
                     /locus_tag="IU325_20925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811461.1"
                     /translation="LLFLYINKDNIKVSYSLKINQTTPGILVSCDSNNNFACQTTMTE
                     DVIQRITTFFHTSPDVKNREIKLEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGR
                     QVLDEIQTPSAINSELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGF
                     FLSIVISVMFCLVKKMVDEYQQNSGQ"
     gene            complement(805..1500)
                     /locus_tag="IU325_20930"
     CDS             complement(805..1500)
                     /locus_tag="IU325_20930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HAP5811462.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTALNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNVVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1493..2920)
                     /locus_tag="IU325_20935"
     CDS             complement(1493..2920)
                     /locus_tag="IU325_20935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HAP5811463.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNVRKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDNVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2931..3650)
                     /locus_tag="IU325_20940"
     CDS             complement(2931..3650)
                     /locus_tag="IU325_20940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HAP5811464.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNTLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLMLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(4178..5032)
                     /locus_tag="IU325_20945"
     CDS             complement(4178..5032)
                     /locus_tag="IU325_20945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP5811465.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFVAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            5259..6584
                     /locus_tag="IU325_20950"
     CDS             5259..6584
                     /locus_tag="IU325_20950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HAP5811466.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTRLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENASIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT         1833 a         1637 c         1435 g         1757 t
ORIGIN      
        1 gcttttattt ctgtatatca ataaagataa tataaaagtg agttacagcc taaaaataaa
       61 ccagacaaca ccaggcatac ttgttagctg tgatagcaat aataactttg cctgtcagac
      121 cacaatgact gaagatgtta ttcagcgaat tactacattt tttcacacca gcccagatgt
      181 caagaacaga gaaataaagc tggaatggtc aggagataag agagatttac caactgctga
      241 agcggaaata tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag aatatcataa
      301 tggcaggcaa gttctcgatg agatacaaac gccttcagca attaacagtg agctttatac
      361 aaaaatgata tacctgacca ggaactggtc attgtatccg aacggtgatg gctgtgtaac
      421 tataagctca ccagaaataa aaaataaata ccctgctgcc atttgcctgg ctctgggatt
      481 ttttctaagc attgtaattt ctgtaatgtt ttgccttgtc aaaaaaatgg tagatgaata
      541 ccaacaaaac tctgggcagt aataaacagc attctgacag agagtgcctg tataaagctg
      601 aagtgacaat gaccgggaag ggaacccgtt ttcaggaatg gatgcctgtc tttttcactg
      661 taaatatcct ggcattacta atgccaggat atttacagaa gagcagcata accatgacgg
      721 aacggggtta agtcaggttg tccttaacgc tattcacaac ctcgtatgct gccttcaggt
      781 ttatgtccag acttcatatc tctctcagca atcctcaata atcagataca ccgctttcac
      841 cgggccatga accccgacga ctttgataag ctcaatatcc gccgttgaac tagggccgct
      901 aatgatgtta atgcaggacg gcattcgttc accggcctgc gctttctgat gcaatttttc
      961 tgcgagttgc gctacacgcg gcaggattgt gcttttacgc aggataaaaa gagaagattc
     1021 cgggagcagg ctcaatgaac gcccgcgctc ggcggcggaa aaaagaacca cgcctcccga
     1081 ttcggttaaa ccatattcag catacacgac acccacttta gcctgctctg cctgtgagat
     1141 attctcggca cctttcgccg gatcccaaac aacgacattg cattcctgct gcaaacgttc
     1201 gctaatcccc aattcctcca gcctcgtgtc accgctaatc acgaccgact gatctcccag
     1261 ctctttacac agacgtattg cagcttctgc cgccttcgcc tcgctggtca actcacagcg
     1321 cgtcaacata acatcgctgg caaactgaat aaacgcgtca cagcgctgct gttggttaag
     1381 tgcggtaagc cgctcgttag catagttgtt aagcggcgca tcttctgctt gcggttcaag
     1441 tcgcagcggg cgacccagtg cctgagcaac gttattcaaa aattcgctcc gattatccat
     1501 tctttttctc ctgcgcctga tgtttcttaa accaactacg gaaactctct ccgtcagctt
     1561 caggaagatc gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt ggtgttttgc
     1621 cgccattgat aaaccaactt gccgcatgag cgccggccat catcccgact ttccacaatc
     1681 ctggatgact attggcataa gcgaacattt ttatcgcccg ttgctctgct tttgcggtga
     1741 tccctttttc agccatcacc cgacgatgac gcaaaatcag ttttgacagc ggaatacgca
     1801 ccggacacac gttgtcacaa gcagtgcata aagagcaggc gtagggtaaa tctttaaaat
     1861 ctttatagcc gccaagtagc ggagaaatca ccgcaccaat tggccctgga taaatagagc
     1921 catatccatg accgccaata tggcgatatg ccggacaagt attcatacaa gccccgcagc
     1981 gaatacagcg cagcacatcc cgaaattcag aggccagcac ctcagaacgc ccgttatcga
     2041 caataaccag atgaaactct tcaggaccat caacgtgccc agcttcgcgc ggtcctgtca
     2101 gccaggtgtt gtatcccgtc aaacgtgcac caacggcact gcgcgccagc atggtgatca
     2161 atacatctac ctcggcaaac gtgggagcaa tacgctccat tcccatcact gcaatatgcg
     2221 ttttaggcag cgtggtacac attcgcgcat taccttcatt ggtcaccagg cataccgaac
     2281 cggtctctgc caccgcgaaa ttacagccgg taatacctat ttcagcactg aggaaatctt
     2341 cgcggatttt ttgccgaatg aataaggtca ttgcttcagg cgtttccggc ccctcatagc
     2401 ccagacgttc atgtagcact cgacggatct gatggcgatc tttatgaatt gccgggacca
     2461 caacatgaga gggcggatct tgatccagct ggagaatgta ttcacccaga tcggtttcaa
     2521 tcacctgaat cccagcatcc tgcaacacat gattgacacc aatctcttcg gtcaccatcg
     2581 atttagattt caccaccttc cggacatttt tgcgttgggc aacctgtaaa atgtagcggg
     2641 tagcgtcttc tttggttttt gcaaaataga cgtgaccgcc gttttgcgtc actttttctg
     2701 agagctggta cagataagcg tcgagattac tcagaacatg atcacgtatc tgggcggccc
     2761 gatcgcgcca ctcctcccag tgccccaatt catcgaccat tttttgccga tttgccccga
     2821 tacgctgctg cgcgtttgcc accgctttgc gcatgatagg atcttcaatt tgctgacgga
     2881 tgcgtgtctt aaaatctgta ttactggttt tgatcgacat ctttatatcc tcagcggctc
     2941 atcaacactt cagcaatatg catcactttg actttctgcc cttcccgttg taatcgtcca
     3001 ctgatgttca gcaggcaact cacgtcagca ccaattaaat actctgggcg gacttccatc
     3061 aggtgcgcaa ccttttcttt caccatctcg ccggatattt cggccatttt gaccgagaac
     3121 gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg tcaacagctc cagtccacgc
     3181 acatttttca gcagcataag tggctcgtcc ttcaccccca gcttacgggc caggctacaa
     3241 gatgggtgat acaccgctct cccttgcaaa ctggcaccta catcgactac ccctaatgta
     3301 ttaacaataa aagaggtgag atcctgcata cgcgcggcaa ccttttcggc acgtgatgcc
     3361 cattcaggtt catccgccag ataagttggg taacttttta cggcataggt acaggagcca
     3421 gccggtgaaa taatgggatc gtcgttatcc tccagtgcgg cgatcagatt tttcatccct
     3481 ggaatcgctt ctttgatata accactattg atcgcaggct gaccgcagca tccctgtttc
     3541 tccgggaaat ttacgcgaca gccgagtttt tccagtagca gcacggagtc tcgtgccatt
     3601 cttgatttca gggcgtcacc aatacaggtg acaaagaaat tgacattcac aactaatact
     3661 ccattacttc atgcccattt atgcccgatg gttcccccct tccgggggat caatcatcaa
     3721 taaatatggc gttctttttt aattggctag caacatacca cccctcattt gtatgacaaa
     3781 tgataatctt tcatccaaac ttcaaatgca ataactaatt ttatttcttt cggcatcgct
     3841 cacattttat agggaaatcg acgatcattt ttggcataag aaatccattt aaaaaggcta
     3901 catcccttat acctattcag tgctacttat tcaggggtta ttgagatata cactgatggt
     3961 gtacaaacaa aatcaccaac aataattacg tatcaagtaa taatcggcat tttatttaat
     4021 tgaagcaatt tagtttgcat ttttatttaa aaactgagct agagtccatc agaaaaaaat
     4081 atataaagcc caaaccaccg ttggaaatag aaatagttga aaggctcaca actcccggaa
     4141 tgcggattat ctttgacctg atttctggcg gttttattca gggtgcaagc tgtctgaccc
     4201 tttcccgata ttctcccgga gtacaaccaa actcgcggac aaacgccttg tgaaaagatg
     4261 attcactggc atagcctact gactcagcga tcaccacaac agggagcatt tcccgggaaa
     4321 acatctgggc cgctatttgt agacgcaact ttgttaatac agctagcggc gtggttccgg
     4381 aaacatcacg gaaaagctgg gcaaaacttg cccgggacat gtgggcgatg ctggccagcg
     4441 attcgacggt ccaggcgtgt cctggcattt ccagcatttg ctgtattacc gcaccaagac
     4501 gtggatgcag aagcaaactg agaatgtttt tctctgtatt aacctgtgca atccactcac
     4561 gcaccgcgag ggtaaagaat gtagcgcaga tctggctaca cagagcatcc actcccggca
     4621 ttgccgatct ggattcctgt tgtaaaaacg ggatcgcctc cctcagccag ttatattcca
     4681 cgctatggtt cactggtgct acaaacagcg tttccggcaa agacgttaaa aaataacgcg
     4741 ccgaatgttg cagccgaaga gtgccacaga caatacaggt cgattcatta tcgacatgac
     4801 tcaggcgatg agctgaattt tgtggtagca ggaccacatt tcccgggcgt aatgtaaaaa
     4861 tctctcccgt cggcatttcc agcttcgccg ctccttgcgt taacgcatgc caacgaataa
     4921 ccgacaattc cccggcacca tgtggaagct gccagtcact tcctaacacg caattcttat
     4981 cgatcgttcc ttgtggagcg ttaagcatca acaaacggct aagagcatcc atatcagact
     5041 cctgagtagt tttttaatta caaacaccaa ctatcttagt caataattaa tctaatacca
     5101 tcaaatagtt aatcatgatt tttgttttgt gattctgatt aagaaaaaga aaataccaga
     5161 cgataagaca aaatatcaag cgttgtgaag aaatgttatt tgctcttttg cgtctatagt
     5221 catgatgtca aatgaacgcg tttcgacagg aaatcatcat gaataaatat caggcagtta
     5281 ttattggttt tggcaaggct ggaaaaacat tagccgtcac gctggcaaaa gcaggttggc
     5341 gtgtggctct catcgaacaa tcaaatgcaa tgtatggcgg gacctgtatt aatatcggct
     5401 gtatcccaac caaaacattg gttcatgacg cacagcagca cacagatttt gtccgtgcca
     5461 tacagcgtaa aaatgaagtg gttaattttt tacgtaataa gaattttcat aatcttgcgg
     5521 atatgcccaa tatcgacgtg atcgacggcc aggcggagtt tatcaataat catagcctgc
     5581 gtgttcatcg gcctgaggga aatctggaaa ttcatggcga gaaaattttt attaataccg
     5641 gtgcacaaac cgtggttccg ccaattcctg gaattaccac cacgccagga gtatatgaca
     5701 gcaccagatt acttaatcta aaagaattgc ctgggcattt aggtattttg ggcggcggat
     5761 atattggcgt tgagttcgcc tctatgttcg ctaattttgg cagcaaggta accattttag
     5821 aagcagcttc gctgtttttg cctcgggaag atcgggatat tgctgataat atcgcgacga
     5881 ttttacgcga tcagggcgtc gatataatcc tcaatgccca tgtggagcga atcagtcacc
     5941 atgaaaatca agtgcaagtg catagcgagc acgcccaact ggcggtggat gcactgttaa
     6001 tagcttccgg tcgtcaaccg gctaccgctt cgttacatcc agaaaatgcc agtatcgcag
     6061 taaacgagcg cggggcaatt gtcgttgaca agcgattaca taccaccgca gacaatattt
     6121 gggcgatggg agatgttacc ggcgggctgc aatttactta tatttcactg gatgattacc
     6181 gcattgtacg tgatgagtta ctgggtgaag gcaaacgtag tactgatgat cggaaaaatg
     6241 tgccttattc cgtatttatg acaccgcccc tgtccagggt tggtatgaca gaagaacaag
     6301 ccagagagag tggtgctgat attcaggtgg tgacattgcc tgtagctgca attccgcgtg
     6361 ccagagtgat gaatgatact cgtggggtat taaaagcgat tgttgataat aaaacccaac
     6421 gtatattagg ggcatcactg ctgtgtgttg actcccacga gatgattaat atagtgaaaa
     6481 tggtgatgga tgccgggctg ccttatagca tattacgcga tcagatattt actcatccgt
     6541 cgatgagcga atcactcaat gatctatttt cattagtcaa ataaactcaa aatcagacgc
     6601 cagaacaaat attctggcgt ctcagagaaa agaatcttat taattctctg taactctata
     6661 aa
//
LOCUS       DACBVI010000206         6647 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.207, whole genome shotgun sequence.
ACCESSION   DACBVI010000206 DACBVI010000000
VERSION     DACBVI010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.207"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            212..1306
                     /locus_tag="IU325_20955"
     CDS             212..1306
                     /locus_tag="IU325_20955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase A"
                     /protein_id="HAP5811467.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1330..1542)
                     /locus_tag="IU325_20960"
     CDS             complement(1330..1542)
                     /locus_tag="IU325_20960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 family protein"
                     /protein_id="HAP5811468.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1802..2110
                     /locus_tag="IU325_20965"
     CDS             1802..2110
                     /locus_tag="IU325_20965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2755 family protein"
                     /protein_id="HAP5811469.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHVPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2168..3262)
                     /gene="yaiW"
                     /locus_tag="IU325_20970"
     CDS             complement(2168..3262)
                     /gene="yaiW"
                     /locus_tag="IU325_20970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HAP5811470.1"
                     /translation="MSRVNHLSSLSLLAVLVLVGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3275..4495)
                     /gene="sbmA"
                     /locus_tag="IU325_20975"
     CDS             complement(3275..4495)
                     /gene="sbmA"
                     /locus_tag="IU325_20975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HAP5811471.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIVGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4847..6004
                     /gene="ampH"
                     /locus_tag="IU325_20980"
     CDS             4847..6004
                     /gene="ampH"
                     /locus_tag="IU325_20980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HAP5811472.1"
                     /translation="MKRCLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNIRPQLDSVIRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6005..6628)
                     /gene="iprA"
                     /locus_tag="IU325_20985"
     CDS             complement(6005..6628)
                     /gene="iprA"
                     /locus_tag="IU325_20985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HAP5811473.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
BASE COUNT         1681 a         1709 c         1605 g         1652 t
ORIGIN      
        1 tcactgcatg gccatacaag ccaggaataa attagcttcc cgttcatcat ttttgtgatc
       61 ttgcgcacat cattgagata acgcgctttc ttcaccagta cgttgcacgc tttgtcagca
      121 gaagattgca acattctgtc agaaggccgc gaatcccccg ttttgtcgtg gtaatgtatg
      181 cactttgtgt tagacgggat aggtttttaa gatggaaaaa ctgcgggtag gaatcgtttt
      241 tggtggtaaa tcagcggaac atgaagtgtc tctgcaatcg gcaaaaaaca ttgtcgatgc
      301 cattgataaa agtcgcttcg acgttgtgct gctgggcatt gataaacaag ggcaatggca
      361 cgtcagcgat gccagcaatt atctgctaaa tgcagacgat cctgcccata ttgcgttgcg
      421 cccttcggcg accagccttg cgcaggtgcc tggtaaacat gaacatcagc ttatcgacgc
      481 gcaaaacggt cagccgttgc cgacggtgga tgtcattttc ccgattgtcc acggtacgct
      541 gggcgaagat ggttccttgc agggaatgct gcgggtcgcc aatttaccgt ttgtaggttc
      601 tgatgttctg gcttcagcgg cctgtatgga taaagatgtc accaaacgtc tgctgcgcga
      661 tgccgggctg aacattgcgc catttattac cctgacgcgc gctaatcgtc acaacatcag
      721 ttttgccgaa gtggagtcta aactggggtt accgctgttt gtaaaaccgg ctaatcaggg
      781 ctcttctgtt ggtgtcagca aagtaaccag tgaagaacag tacgcaattg ccgtcgatct
      841 ggcgttcgag ttcgaccata aagtgatcgt tgagcaaggg atcaaaggtc gtgagatcga
      901 atgcgcagtt ctgggcaacg acaatccgca agccagcacc tgtggcgaga tcgtactcac
      961 cagcgatttt tatgcctacg acaccaagta cattgacgaa gatggcgcga aagtggtagt
     1021 tccggcagcc attgcgccag aaatcaacga taagatccgg gcgattgccg ttcaggctta
     1081 tcagacgttg ggatgcgcag gcatggcgcg tgtagacgtg tttttaaccc cagagaacga
     1141 agtggtgatc aacgagatca acacactgcc tggcttcacc aacatcagta tgtatccgaa
     1201 gctgtggcaa gccagcggtc tgggttacac cgacctgatc acccgtttga ttgagctggc
     1261 gctggagcgt cacgctgcgg ataacgcatt gaaaaccaca atgtaatatt taaacgcccg
     1321 ataatattct tattgggcgt tttcagtttc ttcatcgcga cgacgaataa tcagccccgc
     1381 cagccagaaa ctaatcaccc acgtcaccag accgacggca taggtctgcc aaccttttgc
     1441 ttcaaatccc agtaacccca ccacgccatt aagaatgaat ataaggccaa tggcgaacgc
     1501 atagtagtgc cagtcacggc ggatttttac aggcaagttc atggcagctc cgttaacgaa
     1561 aaacaacatc cttgcatatt ctgcaacatc cgtctgtggc gtagacaaca tttccgtaat
     1621 tgttaataaa tttaacttaa ggttgtcatt ttgtgactta cgacgaagat tcacgcttcg
     1681 ggaatattcc caacatcaat ttaccatgat tctgatattg acaatcttaa tgacctgtca
     1741 gacttgccat aagccgctgg tatcacacca gaagagaagc agatgacttc cggaggtttt
     1801 tatggctgat ttcaccctgt caaaatcgct gtttagcgga aaatatcgca atgcctcttc
     1861 aacgcctggc aacattgcct atgcgttgtt tgtgctgttt tgcttttggg ctggggcgca
     1921 attgctgaac ctgctagtgc atgtgcccgg cgtctatgag cgtttaatgc aggtccagga
     1981 aacaggtcgc ccacgggtgg aaattggttt aggtgtcggc accattttcg ggctgatccc
     2041 gtttttagtt ggctgcctca tttttgcagt agtggcgcta tggctgcact ggcgacatcg
     2101 ccgccagtaa gatttacttg ccagatagga cctaaaacgc tttatctggc atgacattcc
     2161 tgtttgatca ctgtttcata caacgcgccc gccgttcgtc tacgcgcttc gcaaaccagg
     2221 ccgtagtcag gttgcgggtg attttcgggc tttccagttg aatgccaggc aacatttcgc
     2281 ggggtagtga tttacccgtt ttagcttcgg caagttgata aactttcttg tacagcgccg
     2341 tctcctcaaa agagaagctg tcacctttct ctaactgacg gcgaatttcg ctgtcgctca
     2401 tccccagttt tcccgccagt ttgcgcgttg ccagttcggt tttcccgggt tctttactgt
     2461 cgtaacgaat taaatcaccg tccagtgcca gcttcacgcc gctggcctta ctgactgcgt
     2521 tctgaaacgc ggcattacgg ctggcgtacc agccagcgtt aaaatcagca aaacggtata
     2581 tcggcgcgct atagctggcg ggataattca gtaaatggta agtaccaaac cacaacccgc
     2641 cgcgacgact gaagacttcc tgacggactg taccgtccat tttccacgga taccctttgg
     2701 tatgctgttc ggcaaaggca atgctgacct gcatcggccc accggtgcgc accggattaa
     2761 gcgaaccaaa caacgtctgc cccattggca ccatgctgat cagatcgtca aaaatcgcgc
     2821 tcaattgctt ttctgtacgc accgaatcta accgttcgct atagcttttg ccgtttggcg
     2881 atttgatttt tagcgcggta tgcaccagaa aagcaggaat gtgcatccgt tcagcacgac
     2941 ggtcaatctc ttgccaggcg atcttgctta agcccggaac ggccggatct gcctgataat
     3001 ttgactcctg ctgcgccacc gccagcaccg agcagacgtt ttccagcgtc ggtgccagcc
     3061 cctgactttc aaacgtggtg gcgagatctt tcgcccaggc atcgcggtct ttcacgctcg
     3121 caggcatctt ctgccgcacg acgctcgcca catctatcgc tttttcgccc tttttcaacg
     3181 gttgcggagc ctgactacta caccccacta aaaccagaac cgccaacaat gacagcgaag
     3241 atagatgatt gacgcgcgac attacttctc ctttttagct caaggtatgg gttacttcct
     3301 gaattttgtc accatccagc tcatgttcaa agctgcgcag acgtttgtag atagacatca
     3361 actcaaccag tgtggtccat gagttaatca ggtactggaa agctccacga acctgaccaa
     3421 aaacgttggt aatctgcgtc atcaggccga gcgtaatcgt accggcgaca attgacggaa
     3481 acagcaagaa caaaccgaaa acgttatcga cctgcaaata gagaatgcgg gcgatgttga
     3541 aatacatata gtgaaaatag aggcggaaat agtttttccg tacggcgcta aacagctcgc
     3601 gtaccgtagg cggcgtcgcg cgcgtggcat cgtcttcacc ataaaccagc tctttacggt
     3661 aggcagcctc tacacgctgg tttttaaact ccagccccgg cagtttgatc cctaccactg
     3721 ccagtaatcc ggtccccatc agcgaccaga cgatagcggc aatcaccaga ccatacggaa
     3781 tgtgcccgac aattggcagc tccggcacat gcgcggagag cgttaccagc accggcagga
     3841 aggcgatcaa cgtcatgatg gcgttgataa aactgacgcc catattctcc agcgttgaag
     3901 caaaacgcat ggtgtcttcc tgcacacgct gtgcggcacc ttcgatatga cgcagttgtt
     3961 gccagttcgc catgtaatat tcgttcatcg ctgtacgcca gcggaacacg tagtgactga
     4021 caaagaagtt gttcagcaca ctgatcacca cagcgatcag cgcaatcccc agaaagacgc
     4081 ccacttcgcg gtaaaattgt tcgatggtga ctttatgcgg cgaacttagc gcggtttgaa
     4141 tcagatcata gaacggcgca taccaggcgt tgacggcgac cccgacttcc accaaaaacc
     4201 aggtgacgaa gatgatcagt gcagtaccga gtattgacca gtattgccaa cgatgcgggc
     4261 tgtagataaa ccagaacaat gcaaagagtc ctacacaaac aatgtagtaa gcgtaaaaaa
     4321 tcaggaaatc caacgaccag aaacgcgcgg cgctaatcgg gatttgcccg gaagctccgg
     4381 tgatacgcgc cacccaatca cccccacctg cttgccagaa gataacggca atcaatgccc
     4441 aaacaaaggc cgagagaaaa aacgttcccg gctttgggaa aaaagactta aacatatgca
     4501 ctcctgctaa cttcttatcg ttttaacaac tgctgtgtat taaccgcatg accgctacaa
     4561 agcgacagcc aaatgaaacc gcccattgtg acccatagca aggcgcttag ttcgccagcc
     4621 tgagcataag aattgtttcg acggatttca caaaccaaag attgtttaca taagcggatt
     4681 aacacgcgca ggatcacaaa tgcaggcatt ggtgagcagg aaaaagatcg tgtttacgcg
     4741 ccgttacgtt tttaacacta ttattgcact aattcgccct ttgcaatcca tcaatgagta
     4801 gtataaatac gctcagttac cttcattcaa tctatggaca ccaccgttga aacgttgtct
     4861 gcttttttct gccgtgctgt gtgcggcgtc attgacctcc gtccatgcgg cgcagccgat
     4921 cactgaaccg gagttcgcct ctgatattgt cgatcgttat gccgatcata ttttttacgg
     4981 cagcggtgcc acggggatgg cgctggttgt tatcgacggt aaccagcgcg tctttcgcag
     5041 ttatggcgaa acgcgacctg gtaataacat tcgcccgcag ctggattcgg tcatacgtat
     5101 tgcttccctc accaagctaa tgaccagtga gatgctggtg aaattgctcg accaggggac
     5161 cgtgaagtta aacgatccgc taagtaaata tgctccgcca ggcgcacgcg taccgaccta
     5221 caacgggacg cccattacgc tggtgaatct ggcaacccat accagcgccc tgccccgtga
     5281 acagcccggt ggcgcggcac atcgtccggt atttgtctgg ccaacgcgcg agcaacgctg
     5341 gaaatacctt tccactgcca aactaaaagc cgcgccgggc agccaggccg cgtactccaa
     5401 cctcgcattc gatctgctgg cagatgcact ggcgaatgcg tccggcaagc cttataccca
     5461 gttgtttgaa gagcagatta cccgtccatt ggggatgaaa gacaccacct acaccccctc
     5521 gccggatcag tgccgccgtt taatggttgc tgagcgcggt gccagtccgt gcaataacac
     5581 gctggcggca attggcagcg gcggagtcta ttccacgcct ggcgatatga tgcgctggat
     5641 gcagcagtat ttgtcatctg atttttatca gcgcagtaac caggcagatc gcatgcagac
     5701 gttgatttat cagcgagcgc agtttacaaa agtgattggc atggatgtcc ccggcaaagc
     5761 ggatgcgctt ggtttaggct gggtatatat ggccccgaaa gagggtcgtc cagggattat
     5821 tcagaagaca ggtggtggtg gcggtttcat tacctatatg gcgatgatcc cgcagaagaa
     5881 cattggcgca tttgtggtgg tcactcgttc gccattgacg cgctttaaaa atatgagcga
     5941 tggcatcaat gacctggtga ccgaactgag cgggaataaa ccgttggtca tccccgcgtc
     6001 ctgattaata ctctgaaggc aaacggttga tagcgaccag tttgccttta ttcatttcga
     6061 tatagccgcc tttacgtaaa gcagcgagaa cttctgcgac gacagaacgc gatatgcgtg
     6121 tacgttgatg aatataattc atcacaccaa tacgtgagcg taattcttca ttccagtcaa
     6181 tcattgataa taacgttgcg cggatttgtt cgtaggaatt atgcccaatg agctgtacgt
     6241 cacgtaattc cagaattcta ttttgccagg ctaaccagta aaaagcatct cgccagagtt
     6301 gattttgttc aataagggta atggtttgtt tggctggtag atgatatccc gtacaatttc
     6361 cttctgatat taatttgtat ggtatatcgt ttttcattaa accatcagcc agtcccataa
     6421 tataaggagc ctgggtaata ccgataagta cgttttcttc tctacgcaga gagataacgc
     6481 cctctaaaat aacgaaagta tcttcgctat tgacatcact tgaaaatata acttgctttt
     6541 cattattaaa ctcgaagcgc gtgccatatc tggacaaaca tttatcgagc ttaccaaatt
     6601 cctgaagagg tttaactaca gataacattt gcgcgtcctt tgcagta
//
LOCUS       DACBVI010000207         6592 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.208, whole genome shotgun sequence.
ACCESSION   DACBVI010000207 DACBVI010000000
VERSION     DACBVI010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.208"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..122)
                     /locus_tag="IU325_20990"
     CDS             complement(<1..122)
                     /locus_tag="IU325_20990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005188750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HAP5811474.1"
                     /translation="MNERKRFIRLPEVLNRTGLCKAWIYRLISRNEFPAQVKLG"
     gene            complement(416..745)
                     /locus_tag="IU325_20995"
     CDS             complement(416..745)
                     /locus_tag="IU325_20995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HAP5811475.1"
                     /translation="MKDRIVEILQYTLKKDSGEEFHQIMHEVSVPLHTRNGIDVVAYG
                     NSLHDPDSYYLIRAFESEEQMESILDDFYASAGWRSGPREAIISRIELSLKSVLSLPQ
                     SVIDGLR"
     gene            complement(1175..5738)
                     /locus_tag="IU325_21000"
                     /pseudo
     CDS             complement(1175..5738)
                     /locus_tag="IU325_21000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(5803..5922)
                     /locus_tag="IU325_21005"
     CDS             complement(5803..5922)
                     /locus_tag="IU325_21005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP5811476.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(6076..6219)
                     /locus_tag="IU325_21010"
     CDS             complement(6076..6219)
                     /locus_tag="IU325_21010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811477.1"
                     /translation="MWHLVVLLEELCKRGINFRALAQSIFPQQWGDECCKSKTICDLK
                     VIV"
BASE COUNT         1608 a         1684 c         1410 g         1890 t
ORIGIN      
        1 tcaccaagtt ttacttgggc cggaaattca ttacgactaa tcaggcgata aatccaagcc
       61 ttacaaaggc cagttctatt taatacttca ggtaaacgga taaatctttt tctttcgttc
      121 atatatttcc tcgtcgttgt tttgatgagg ttattattcg aatatcgctt atgacaatca
      181 acacctgttt tttcggaatt attgcgtaag tgagtgattt ttgtacaaat cacaaaggaa
      241 tagattgaat gctgttacaa gacgggactt agagggcttt agcaaaaagt gctattttta
      301 gaagcggaaa aaggatttta gaaatgtatc tatttatcaa ttaattatga taattcagtt
      361 taaattctgt actgcggagg ataccccttg aacagcgcat ccccctttgc tcactttatc
      421 tcaatccatc aataacgctt tgtggtagtg atagaactga ttttaaactg agttcaattc
      481 gactgatgat tgcttctcgt gggccactac gccaacccgc actcgcatag aaatcatcaa
      541 gaatgctttc catctgttct tcactttcaa aagcccgtat gaggtagtag ctgtcaggat
      601 cgtgcagaga gtttccgtac gcaaccacat cgataccgtt ccgggtatga agtggaacac
      661 tgacttcatg catgatctga tgaaactcct caccagaatc ttttttcagg gtgtattgta
      721 gtatttcgac gatcctatcc ttcatacctt ctccatcaat gaccatgaga aactgtttca
      781 atgacgctaa cgcattcggc tggttaaagg aagaaaagct gacacggatg atctgagttc
      841 aacttccagt tggtagctga actgttgcat gtattatctt tcggcatagt ttgacgtcaa
      901 acgttcagca atttcgctta atggttccat aattgaaccc tcaattgaag aaaaacataa
      961 aatatacata taaatcaata tattaaaact gtagttacag ggacgccagc cccaatcatg
     1021 attggacggt gcaaggacaa caccaacaaa aacaggaagt tagaagtctc agcaagacac
     1081 cgaccagacg gtgaggagac ataaaaggat acgcaaagga gccgcggctc ttggtgatat
     1141 gaaagcccac agatgtgggc tttttcgttg atggtcagaa cgaccagttc gcaccagcca
     1201 ccgcgttcca cggggattcc acaccggcac catggctata ccccaccccg agatgtccgc
     1261 ttaacgtact gctgaatgag gctttaatac ctgcctggta tattccacgt ctgcccgaca
     1321 aatcattgac gaaattaccg tcattattca ccttcacccg gttatcatcg acaaattctt
     1381 tgcgcacagc cgccttcagc cacggctcaa tttccatacc gttccccaga cgcatgttgt
     1441 aactcagcgt tgcgcccagt tcacgatata tactgcgggt atcgactgat ttcgattcca
     1501 tgccattgga taaatgatat tcggggttat cagcggtgaa ccccgttaac gatgcatacg
     1561 gcgtcaggtt ccagttacca tccgtaaatc gcatcccggt ttcaatgtga ccgcccagcc
     1621 cgttgctgcg ataactgccg ttggcggctc caccgctgct cattttaccg gctacgttac
     1681 tttcaaagcg gttcagcttc acgataccgt ccagatagaa accactttca tgttcccagc
     1741 tggcatagcc ccccagagaa taactgccca cactgccatg tcctccacga tcaaaaccaa
     1801 tatgtgaatg ggaataaccc ataaaagcgc ccagcgtcgc aatcccctca ggaatatcat
     1861 tacggctatc gatccccacc gtcattccgg tcagcgtctg ctcaaacccg gcacccgcat
     1921 cggtggtgac attattacgg gtgttatacg tcgtccccca gacattattg ttgtgtggac
     1981 tcgctttcat tatgttcaac cgctcgcgaa tactgtttag ctcagcatca aagaccaacg
     2041 gtaatgttgc tgccatattg agtacggctg ctgtagaagg cgtgatgcgt ttctccggga
     2101 cgggtgtcgg cgttggagcg ggagtcgggt ctggtttcgg atccggtttt gggtctggtt
     2161 ttggatccgg tttcggattt gggttggggt ccgggttggg tttgacatca ttggtcaggt
     2221 tccagttgct gttgccgtcg cttttcagga catactcata ggtcccaaga tcaacgaaac
     2281 cgccggtatt acccagcgta aacgaagtat cccctccccc tgttttcacc agcgtcatcg
     2341 cgtcgtcaga ctgagggctg acgccggtat cctgaacaaa gattttaaaa ttgccagtgg
     2401 cgttgttgtt gacgaccagt tgatcgcccc gggagcctgc aacgttggta tgcaggtaga
     2461 aattaccgct gccggaaagt tcactggtgg tcagcgtatt gtagataccg ttagttgtgg
     2521 caggcgctgc tgtttgtgct gataaataaa catcaccgtc gttgaggagt aaactgttta
     2581 ctctatattc gcagttgctg gtgcctgcac aggaattatt gctgttaagc cagacactgc
     2641 cccgactggc agccgtcagg tcggcaagcg tcgtatcgac gccatttccg atagttaatg
     2701 tcgcgctatc ggtaatccgg accacccctt caagtttaac tggcgtaaca ttatcccgtg
     2761 gcgtcattaa cgacaggctt cctgtcgcgc cactgaccga tgcatccgcc agcgtacctg
     2821 cgtagacgat tgccgtcccg ccagcgatca gtagatcttc tgcccgggcc agagcctgaa
     2881 actcatcttt tgaccgacca agggtatatt gcccaccgga attaaccttt gtggcgaagt
     2941 cctgacccag gttttgcatt gccccaccgt tgccaaccgt ggaatcgcga gcttcggtac
     3001 ctgctaatac caataaatta ccgccatttt ccagcagcac attggtcgct aaatttccgg
     3061 caatggaaaa ggtgccgtac tggtgagtac cgctgatttc aataccgtta gccgtgctcg
     3121 tctggagagc ggcaccgctg ttctggatga tatctgtcgc tttgccatta tcgttgactg
     3181 tcagcgtacc gccttcatta atcgttgttt ttactgcctc accgttagcc gaaactgttt
     3241 gtattccgcc gtcgttaatc gttgtctcat tcgccacccc ctcgacgatt tgttcgccgc
     3301 cggtgagcgt cgtgcctgtc gcagtggctt ttgttttgac gatctcccgc ccgcccgcat
     3361 tgacctgtgt tttggcggaa gtggtgtctg actccacggt taacacgccg ccatctgcca
     3421 gcaggatatt gttcgccgca tcctgctcaa tgctgaacgc gacgccatca gcgcgtgtcc
     3481 ctgtgacccg cgtcgctctg gtggttgcaa ccaacgcgcc ctggctactt tgctgtatcc
     3541 ccgttgcgct gcctttctcc cgcacatcga gagtgccgcc ttcattaagc accgagtttt
     3601 cagccagacc gccctcatta actacctgtg aaccgccatt aataatggaa ccttccgctg
     3661 tcccgtttac cataatttgt tgtaggccag agacgatatc ggtattgatc gccttaccat
     3721 aattctgaac ggtttgcgta ccgccattga tgtgcgtttt gtctgttgac ccaccatcaa
     3781 caatttgttc accactttcg atatttgcct cagtggctaa accatatacc gtttgcttgc
     3841 cacctttgat atttgctttt tcagaagtgg cactggcata tattgtttgg gtaccagcac
     3901 tattaagtac agtaccaaca tcttttccat aaacatccat tttgccgttg gcattaataa
     3961 tcgtgtcaac tgcccgggaa ccagtgacga ctgttaatga gccagcgttt tccagcacta
     4021 catttttagc ttctgaattc ctgatgtaga aagcatcacc ataactgttg gttccttcga
     4081 taagtgttcc ggaggtcgtg gaagcaatta atgcgccgcc ggattgttgc tcaacatgct
     4141 tagcctcacc accgtcctga acctccagaa cgccaccatt attaagtctg gtgctatctg
     4201 ttttagcctc cttctggaca atcagcttac cgccagcatc aatggtagta tttttcgccg
     4261 aggttttagc cattaccgtc agttcgccgg tattttccag cacaacataa ttagcctctc
     4321 ctccggtaat agtgaagtga gagagcttgt tgtatccttc gatatcagtc cctgcgcccg
     4381 tgttggcaac taaagcactg cccgtctcca tgtgcaatac cgccggtata gacaatcagc
     4441 gagcctccgg cgctaatatt gctgccagtt gccgtaccat ctttctcaac aacctgccgg
     4501 ctcccagagg atatatttgt cgtgtcagct ttcccgccgc tcttgatatt ttgcgttcca
     4561 ctgttgatat tggtgcccgt ggcaatacca tgattattaa tattctgtgt gccaccatta
     4621 attatggtat ttgtcgcgtt tccggcaaca tccataacgc cgccattatc tattcgggtc
     4681 gcatcagctt tagcattcgt taaaactgac attgttcctt tatctttaat aatcgtctta
     4741 tttgccgaac catatgcgtt tatgtctaaa tgaccaccgt tttccagcaa cacattctct
     4801 gccacgttat tgtggatgga gaatgcccct tcactattcg taccgctcac cgtcaaatcg
     4861 taagtcgtta cttttaaagc ggctccatcg tgctgagtaa tattagttgc cgtaccacct
     4921 ctaacatcaa gcacgccgcc ggaataaact tcaataacat ccgaggagct cgtgttatca
     4981 acaatttgcg taccaccaga atagatctgc gtattttttg ccgttgactt actattcaga
     5041 gactgagttc cgccttcaat cgtcgtgtcc agcgcacggc tctcatatac tctttgctca
     5101 ccgccatttt taatggcggt tgtttctact gtgctctgtt caacatactg tcgcccacca
     5161 tttatggttg tgttcgtcgc cagacttcct tgcgtgatgt cctgataacc agacttattt
     5221 atcgtcgtac cgtcaacatg cccctgagtt gatactgact ggcctccacc atcaatgatt
     5281 gttccatttg cagtcccccc ctctatgcgt gaagtaccgc ccttaattgt cgtgtcattg
     5341 ctggtaccac ctttataaac atattgattg ccactttcga ttatcgtgcc agtagaaatt
     5401 cctccgtact caattgactg tctgcctcca ttaatggttg tattattagc ttgcccgtta
     5461 atatcggcat ggattgatac tagttgctcg ccaccagttt caataatact actattagat
     5521 atcccaccat agacctcctg ccggccatgg ttgataatat gagtgttatt tgttgtacct
     5581 cgttcatcta ctcgttggtt gccatctaca gtctcatcgt ttaccacacc aataacatta
     5641 gtagtgaagg ccgccatccc gggctgggca tatattaagg cagatatcaa taaggaaagt
     5701 actgagcggc gaaaataata gggactggtc ctgttcataa atttcatcct ctgaaaagtg
     5761 aatactgagt agcgtttaag cgaccttagc tttgctgcaa catcagccca caggcaccag
     5821 accaggggat tcatcctgaa gagacagcgc aagtgtattg tgttcaccgc tcatcaaaga
     5881 catcatgatg aaatgatgat attccgcata agaatgaggc atttttttaa gacattgtgc
     5941 tgaaggatga ttggataaaa aagtcaatct tttcaggaaa tacggcgtat tcttttcttt
     6001 cgacattgaa gccgtcggaa aaataaaatg atttacataa tcgtttctga tgaatatctt
     6061 ctgctcacat aaaaatcaca caataacttt gagatcgcag attgttttac ttttacagca
     6121 ttcgtccccc cattgttggg gaaatataga ttgggccaga gcacgaaagt taataccacg
     6181 tttgcacagc tcctccaaca gcacgacaag atgccacata ctgcgcccca gtcggttcag
     6241 tttacagact agcagtgcgt accctgccga taatgtcctg agcagttttt cagtccgggt
     6301 ctgctgccac ggttatgcgc tgaagtggta actctgagca cgcagttcat cagtcaggcg
     6361 tggtacaccg taacgctgtt attgctgggt aatatcaaaa aaactttcag gcagctaagg
     6421 aaagttgaac cagacattag atgaaatatt tcaaccaatt acagcaccaa ttcagtcact
     6481 gctagcccac caaataaatc aaggggttac atgaaaacgt agcccctttt tctttggtag
     6541 tgacactaaa atggatgtag tgtgaagaat aaccccattt acttaatcaa ta
//
LOCUS       DACBVI010000208         6519 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.210, whole genome shotgun sequence.
ACCESSION   DACBVI010000208 DACBVI010000000
VERSION     DACBVI010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.210"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            1..250
                     /locus_tag="IU325_21015"
                     /pseudo
     CDS             1..250
                     /locus_tag="IU325_21015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(449..1573)
                     /locus_tag="IU325_21020"
     CDS             complement(449..1573)
                     /locus_tag="IU325_21020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP5811478.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(1573..4308)
                     /gene="rbbA"
                     /locus_tag="IU325_21025"
     CDS             complement(1573..4308)
                     /gene="rbbA"
                     /locus_tag="IU325_21025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HAP5811479.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPTRCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVIVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSSEAEAPPVVHDATHAPRQGFSLRRLFSYSRREALELRRDPVRSALALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPSSQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(4305..5372)
                     /locus_tag="IU325_21030"
     CDS             complement(4305..5372)
                     /locus_tag="IU325_21030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HAP5811480.1"
                     /translation="MDKSKRHLAWWVVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(5738..>6519)
                     /locus_tag="IU325_21035"
     CDS             complement(5738..>6519)
                     /locus_tag="IU325_21035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAP5811481.1"
                     /translation="NKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHV
                     LAGNHETNCNGNYMQNFTRMKPLDEDTYAGIKDYPVCFYDPKYKIMANHHGITFDDQR
                     KRYIIGPITVSIDEMTNALDPVELAAIINKKHHTIINGKKFKTSRAISCRSFNRYFSV
                     STDYRPKLEALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSM
                     HNCQINPGAGPEITTPWKSYQHEKNKNGLMPLIRRRTMLQL"
BASE COUNT         1552 a         1812 c         1635 g         1520 t
ORIGIN      
        1 agaggatttt ggggaaactc atctcgattt tttgaagcaa tatggtgatt ttgaaaatgg
       61 tattcctgtt cacgatacca ttgccagagt tgtatcctgt atcagtcctg aaaaatttca
      121 cgagtgcttt attaactgga tgcgtgactg ccattcttca gatgataaag acgtcattgt
      181 aattgatgga aaaacgctcc ggcactctta tgacaagagt cgccgcaggg gagcgattca
      241 tgtcatttct cgcgtcagtc cttgcgggga gcaggctttc gtaatcttgc cctgaatacc
      301 ggactgagta tcaaaaagcc ggttaactga aactgtccag gttttggtgg tcagttcata
      361 aacgccttat ccggcctaaa aaaaactaaa attcaataaa ttgcaccaat gcgtagaccc
      421 tataagctta cgcatcgggc aactgtaatt acgccattgt cccaatcgtc ttcctaaatc
      481 gcagcagtgc gatggtgaaa aacgcgccgc caattgccat cagcgtcagg aactgcggcc
      541 agacgatttc gaatccggca ccccggtaga ggatggcctg cgcgaggcta acaaagtgtg
      601 tcgtcggcat ggtcagcata atgtcctgca ccatctgcgg catactttcg cgcggcgtgg
      661 atccaccgga aagcatttgc agcggcagca gcaccagaat caccagcagc cccagttgcg
      721 gcattgaacg cgctatcgtc cccataaaaa tgccgattga cgtggtggca aacagactga
      781 gcgccacgcc cagcataaac agcgggatcg agccttcaat cggtacgccc agcacgcctt
      841 ttaccatcag caccagcgat aatcccgata ccaccagcac caccagcccc atcgaccaga
      901 tcttcgccat catgatctca aacggcgtta tcggcatcac cagtaagtgt tccaccgtgc
      961 cgtgttcacg ctcgcggatc agcgccgatc cggtcaatac aatcgccagc atagtaatgt
     1021 tgttgatgat cgccatcacc ccgccaaacc acgcgggatc gaggttcggg ttaaagcgca
     1081 tccgggtttc cagcgatacc aacggttcgc tattatcacg gtagcgcgcg acaaagctgt
     1141 tcacttcacc gttgataata ttctggatat acccattgcc ggtaaatgcc tggctcatgc
     1201 gcgtggcatc gacgttcacc tgaatatctg gctggcgtcc ggcgaggaca tcacgctgaa
     1261 aattaggcgg aatatttatc gcgaaggtat agcgtccggc gtccagcccg gcatccatct
     1321 catcggcggt gatcatctcc ggtggcaaaa accacgggcg atagaagctg ttaacgatcc
     1381 tgttcgataa ctgcgattga tccatatcgg caatggcgat cggcgcgagg ttcaacgatc
     1441 ctggcgtaac ggtcgctgac gaatacaccg acaccgtaaa cgagaagaca atcagcgtca
     1501 gcatcgcttt atcaccgagc aaactgcgca actctttgat acccagatta aaaatattgc
     1561 gtaaatggcg catcatccct cctgtttttt cagcagcagg atgcttaagc ccatcaccag
     1621 cgggatggct atcagcaacg ggataaaaag ttgccacaaa tcagtcaaat ccagcgcctt
     1681 cgagaacgtc ccgcgggcaa tagtcagaaa atgactggtc gggtaaacct cgccgatcca
     1741 gcgtcctgga ccttccagcg aagctaccgg atcaatcatc ccggaaaact gtgtcgccgg
     1801 gatcaacgtg ataatcgccg taccgaaaat agcggcaatc tggcttttca taaaggtgga
     1861 gatcagcagc cccattccgg tggcgatgat gatatacagc agcgccgcca gggtgagcgt
     1921 caggaaactg cctttatgcg gtacgccaaa cacaaacacc gacaggccgc agagcaggaa
     1981 aaagttcagc atccccagcg cgatgtacgg tagctgtttg ccaagcaaaa attcgctacg
     2041 ggtggtggga gtcacgtaaa ggttgataat cgaccccaac tctttttccc gcaccacgct
     2101 aagggcgctt agcattgacg ggatcatcat cagcagaagc gggatcaccg ccggaacaat
     2161 cgctggcagg ctttttacgt ccgggttata gcgatagcgc gtctcaatgt tcatcagccc
     2221 gctttggctg gagggtgtgg attgtcggct cgccacatcc tgtagccagc tctggtgcat
     2281 agcctgcacg taacctttta ccgtttcggc acgactgggc atcgcaccgt cgatccagac
     2341 accgagttcc acaggcgtac cacgcgcgat atcgcgcccg aaattgggtg ggatctcaat
     2401 cgccaccgtg atatcgcccg cacgcatccg gcgatccagt tcgtcataac tggtgagcgg
     2461 tggttgttcg ataaagtaac gggaaccgga gagattgagc gtccacgcct ggctactgac
     2521 ggtctggtcg cggtcaagca ccgcaaagcg caggttttcc acatccatac tgatgccgta
     2581 acccattatc agcatcagga tcaccgttcc catcagcgcc agcgccgaac gtaccggatc
     2641 gcgtcgcagt tccagtgctt cgcgacggct gtagctaaac agacggcgca gactaaatcc
     2701 ctgacgcggc gcgtgggtgg cgtcgtgtac cacgggcggc gcttcggctt cgctgctctg
     2761 ccctgccgct tcctgcaaat aggcgataaa tgcctcttcc agactggcgg ctccgcgttt
     2821 ctcaaccaac tcctgcggtg taccgctggc gagcactttt ccggcgtgca tcagtgagat
     2881 acggtcgcaa cgttccgctt cgttcataaa gtgggtggag atgaagatag tcactttgtc
     2941 ctgacgcgag agatcgacca tcaactgcca gaacatatcc ctcgccaccg gatcgacacc
     3001 agaagtaggc tcatcgagga ttaacatctc cgggcgatga atcaccgcca ccgccagcga
     3061 aagccgctgg cgaatgccga gcggtaatga ctccggcaga acatcttcaa cgtcgttgag
     3121 cttaaaacgc tcgctcattt cagccactcg cgcgggaatt tccgcttccg ggatgtgaaa
     3181 caaacgggca tgtaactcaa ggttttgccg cacggtgagt tcgttataga gcgaaaacgc
     3241 ctgcgacata tagcctaccc gacggcgagt atcgatatct tttggatcaa ccggttgccc
     3301 gaacagccac gcctcacctt cgctggcggg cagcaatccg gtaagcattt tcatggtggt
     3361 ggatttaccg cagccgttcg aaccaagaaa accaaaaatc tccccgcgtg gaatgcggaa
     3421 attgacgtga tcaacggcaa cgaaggaacc aaaacgcatg gtcagatcgc gcgcttcgat
     3481 ggcaatctct gcgttttcag gttgatatgg tgggatcact accgcctgat gcgcctggcg
     3541 ttgcgcttgc ggtaacagat ttataaatgc ttcttccagc gtggcgcttt gcgtttgctg
     3601 ccgcagctct tcggcgctgc cggttgccag cacttctccg gcattcatcg ctaccagcca
     3661 gtcgaagcgt tcggcctctt ccatgtaggc ggtggcgacc agcacgctca tattgctctg
     3721 ccgctggcga atactgtcga tcagatccca gaactgggcg cgggagagcg ggtcaacccc
     3781 cgttgttggc tcatcaagaa tcaacagttc cgggtcgtgg attaacgcac agcacagccc
     3841 cagtttttgc ttcatcccgc cggagagttt ccctgccgga cgatcacgaa acggtgctaa
     3901 cccggtgctg gtcagcagct cattaattcg cacttctcgc tccgctttgt cgtgaccgaa
     3961 caggcgggcg aaaaaatcga cgttttcata caccgacaag gtgtggtaga ggtttttacc
     4021 cagcccctgc ggcatccagg cgatgcgcgg gcaaacgtcg cgtcgatgct tcgggtcgcg
     4081 catatcgccg cccagcacga tcacattgcc ctgctcaatg acacgagcac cggaaatcaa
     4141 tgacaataaa ctcgacttcc cgacgccatc cggaccaatc agcccaacca tgcagcgggt
     4201 cggaatatcg agagtgatat tgttcagcgc aacggttttt ccataatgct ggctcacgcc
     4261 cgccagttgc gcgacaggcg ggacgggaac cagttccaga tgcgtcattg cggcaacctc
     4321 accaccaggt cgtcaggcca cggaagttct tcattcaccc gcacccacgc tacgcccggc
     4381 aaaccggttt tgacatattc cagatgctgc tggagtaatt ccggtgggat acgcgctttg
     4441 acgcggaaca tcagtttcag ccgctcatcg ctggtttcga cggtttttgg cgtgaactgg
     4501 gcgacgctgg cgacaaaact gatggttgca ggaatacgca gatctggcgc ggcatcgagg
     4561 atcagccggg cttcaccgcc cagtttcagc gtgcccgcct gttcggttgg caggaagaag
     4621 gtcatataga cgtcgctcag atcgaccata ttcagcaccc gaccgcctgc cgccagcact
     4681 tcccctggct cggcaacccg atactgcacg cgtccgtcac gcggggcttt cagttcgctg
     4741 tcatcgatat ctgcggcaat gcgccgttca gtggcttgtg cggcttcgac gcgtgtttgc
     4801 gcctgaatga tattggtgcg tgccgcttct atagcggctt tagaagccga aacctgggct
     4861 tttgctgatt ccagcgcagc tcgggcgctc tcagcggcgg cgcgatcgtc atccagctgt
     4921 tgcgcagaaa tagcccctcg ttgggcgagc gaacgggaac gcgtatgacg tttggcaacg
     4981 gagtccagtt cggcctggcg ttgattgacc agcgactgtg cggcacgagt ttcgctttgg
     5041 cgttgctcca gcaaagcctg cgcggcggca acggcgcttt gtgcttcttt gatttgcgcg
     5101 atggcttcca gtcgctgttc ctgcaacacg cgagtatcca tcttcgccag cacttcacct
     5161 tcgcgaacaa actggccttc tttcaccaga atggtgtcga tacgcccggc aattttgctg
     5221 gcgatatcca cttccgtcgc ttcaatgcgc ccattgctga cggcaaagcc ttccggcaca
     5281 cccgctgggc gcaacagcca ccacgccacg atagcggcca ccgccagtaa cccgacaacc
     5341 caccacgcca gatggcgctt actcttatcc ataatcgacc cgccataatc cctgtaagca
     5401 aacgacgcgt gttacaactg ccgccgaacg acgttcagcg gattcttcca tccgggataa
     5461 gaaggaaacg gcaaatacga ccagcgcgca gagcattgag cgccaacgtg gaaggcttag
     5521 tgaaaacggg tagtaatgtt gtcatcatca gcattcctgg ccgtaaatga aacacgcata
     5581 ctcataagtg tgatctataa aactggctga caaaacggta tccatcctgt tggatagcgt
     5641 ttcacgcctg ttcagttgca ggaacagagc attccttatt acgttttatc aacagtctca
     5701 accagatagt aacattaata ttcatgttgt ctatggttca gagctgtaac attgtgcgcc
     5761 ttctaatcaa aggcataagt ccatttttgt ttttttcatg ctggtaagac ttccagggag
     5821 tggttatctc aggccccgca ccagggttaa tttggcaatt atgcatgctg aacattcttc
     5881 cagcatgttt actgtcacgg gcgttaagac ctaaaactgt ccccgtttca cctatgcgtt
     5941 ctctcccacc atttccgtta tgtgcaacaa cttgatttat acctaacatt tgtgagcatg
     6001 ctaatagtgc ttcaagttta ggtctgtaat ctgtcgaaac agaaaaataa cgattaaagg
     6061 atcgacaaga gatagcccgg ctagttttaa actttttacc attaattata gtatgatgct
     6121 ttttattaat aattgccgct aattcgaccg gatcgagagc attggtcatt tcatcaatag
     6181 agactgttat cggtcctata atgtaacgtt ttcgctgatc atcaaaagtt atcccatgat
     6241 gatttgccat tattttgtat ttagggtcat agaaacatac aggataatct tttatcccag
     6301 cgtaggtatc ctcatcaagt ggtttcatac gcgtgaagtt ctgcatatag ttaccattgc
     6361 aattcgtttc atgatttcca gctaatacat gaacgttttc attgatactc attatacgtt
     6421 ttaacaaatc gatgataaat tgatcgccac agatattact aaatcgatcg ccggtttggt
     6481 cacccagaaa cgtacatggc gtcacagcgt ctttattaa
//
LOCUS       DACBVI010000209         6284 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.211, whole genome shotgun sequence.
ACCESSION   DACBVI010000209 DACBVI010000000
VERSION     DACBVI010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.211"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            114..1472
                     /locus_tag="IU325_21040"
     CDS             114..1472
                     /locus_tag="IU325_21040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001453872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811482.1"
                     /translation="MFFAILFNGMDAFFFANKRSALAENNPESVTPEHIYHELKEQNQ
                     TLTKLVSGINGDSEGSLIAQIKLLRTEISDSSQAQLANHTHFSNKLWEQLEQFADLMA
                     KGATEQIIDALRQVIIDFNENLTEQFGENFKALDASVKKLVEWQENYKTQVELMSEQY
                     QQSVESLVETKTAVAGIWEECKEIPLAMSELREVLQVNQHQISELSRHLETFVAIRDK
                     ATTVLPEIQNKMAEVGELLKSGAANVSASLEQTSQQILLNADSMRVALDEGTEGFRQS
                     VTQTQQAFASMAHDVSNSSETLTSTLGETITEMKQSGEEFLKSLESHSKELHRNMEQN
                     TTNVIDMFSKTGEKINHQLSSNADNMFDSIQTSFDKASAGLTSQVRESIEKFALSINE
                     QLHAFEQATEREMNREMQSLGNALLSISKGFVGNYEKLIKDYQIIMGQLQALISANKH
                     RG"
     gene            1479..2186
                     /locus_tag="IU325_21045"
     CDS             1479..2186
                     /locus_tag="IU325_21045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAP5811483.1"
                     /translation="MDKIIGKQLPKKDQDNEHWVSMSDLMAGLMMVFMFISIAYMHYV
                     RIEKEKIKEVAVAYENAQLQLYNALDIEFAKDLQDWDAEIDKQTLEVRFKSPDVLFGL
                     GSTELKPKFKLILDDFFPRYLKVLDNYQEHITEVRIEGHTSTDWTGTTNPDIAYFNNM
                     ALSQGRTRAVLQYVYDIKNIATHKQWVKSKFAAVGYSSAHPILDKTGKEDPNRSRRVT
                     FKVVTNAELQIRKIIQE"
     gene            2190..3293
                     /locus_tag="IU325_21050"
     CDS             2190..3293
                     /locus_tag="IU325_21050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP5811484.1"
                     /translation="MKLSIDISELIQLGKKMLPEGVDFFLDESPIDFDPIDIELSTGK
                     EVSIEDLDPGSGLISYHGRQVLLYIRDHSGRYDAAIIDGEKGKRFHIAWCRTLDEMRH
                     KNRFERYHATNRIDGLFEIDDGSGRSQDVDLRVCMNCLERLNYKGSIDKQRKREIFKS
                     FSLNEFFSDYSTCFRHMPKGIYDKTNSGYVENWKDISKSIREKAKYTCNNCGVNLSTA
                     KNLCHVHHKNGIKYDNHHENLLVLCKDCHRKQPLHEGIFVTQAEMAIIQRLRSQQGLL
                     KAESWNEIYDLTDPSVHGDINMMQHKGFQPPVPGLDLQNSEHEIIATVEAAWPGLKIA
                     VNLTPAEVEGWRIYTVGELVKEIQTGAFTSATL"
     gene            3503..3730
                     /locus_tag="IU325_21055"
     CDS             3503..3730
                     /locus_tag="IU325_21055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose oxidase"
                     /protein_id="HAP5811485.1"
                     /translation="MNKAITVLAIMMASFSVNAYVLPETPVPFKSGTGAIDNDTVYIS
                     LGSAGTAWYKLEKQTKDKKMDSVSCISWWTE"
     gene            3750..4112
                     /locus_tag="IU325_21060"
     CDS             3750..4112
                     /locus_tag="IU325_21060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HAP5811486.1"
                     /translation="MDGNLYVFDGIGKNSEGLTQVFNDVHKYNPKTNSWVKLMSHAPM
                     GMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNEAGKDSTYRTPENWTTTPRSSHFSR
                     GMIVDKFVDAILRFWRER"
     gene            complement(4120..4770)
                     /locus_tag="IU325_21065"
     CDS             complement(4120..4770)
                     /locus_tag="IU325_21065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP5811487.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIEQGFHIHLDKAAAAPIADVLFPD
                     ELPSQETYVEGAAIKVTVNRYERDKKARDKAVEHHGCQCNVCGVDLVKIYGDIAEGFI
                     HVHHLVPLSAIKEDYQLDPVNDLLPVCPNCHAMLHRRKPPFTPEELKALMDANKSN"
     gene            4907..5779
                     /locus_tag="IU325_21070"
     CDS             4907..5779
                     /locus_tag="IU325_21070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP5811488.1"
                     /translation="MASSKSLQQAIANIKIWHKDEQRAPHKPLLLLYILAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELTEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYKYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPF
                     EGFVEWHRKEVFRG"
     gene            5824..6003
                     /locus_tag="IU325_21075"
     CDS             5824..6003
                     /locus_tag="IU325_21075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811489.1"
                     /translation="MQDLTASLQLAFDSGAKRVLLPISSAMDIPTVPAELFTKFQVSF
                     YSESVDAVYKALDVN"
BASE COUNT         1954 a         1225 c         1359 g         1746 t
ORIGIN      
        1 ctttcattgg tattattatt gggttactca attttaatac tgaaagtatt gataccagca
       61 tccccgtatt attaggcggc ctaaaaacag cattcattac aagcattgta ggtatgtttt
      121 ttgccatttt atttaatgga atggatgctt tcttttttgc caataaacga agtgcgttag
      181 cagaaaataa ccctgaatct gttacacctg aacatatcta tcatgaatta aaagagcaga
      241 accagactct gactaaatta gtctcgggta ttaacggtga tagtgaaggt tctcttattg
      301 ctcaaataaa attactacgt actgagatta gcgattcctc gcaggcacaa ttagctaatc
      361 acactcattt cagtaataag ctttgggaac aacttgaaca atttgcagat ctaatggcaa
      421 aaggtgctac agaacaaatt attgatgctt tacgacaagt cattattgat tttaatgaaa
      481 atttaactga acagtttggt gaaaacttta aagcacttga tgcctctgta aaaaaacttg
      541 ttgagtggca ggaaaattat aaaacgcaag ttgagctgat gtcagaacaa tatcaacaaa
      601 gtgtcgagtc tctggttgaa acaaaaactg cggttgccgg gatttgggaa gaatgtaaag
      661 aaattcctct ggctatgtct gaactgcgtg aagtgcttca ggtgaaccaa catcaaatca
      721 gcgaactctc ccgccattta gaaacctttg tcgccatccg cgataaagcg acaaccgtat
      781 tacctgaaat acagaacaaa atggctgaag tgggtgaact gctgaaatcc ggagctgcaa
      841 atgttagtgc atctcttgag caaaccagcc agcaaatact tcttaatgca gattcaatgc
      901 gcgtggccct ggatgaaggt accgaaggat tcagacaatc agttacccaa acacaacaag
      961 catttgcctc gatggcacat gatgtcagca attcctccga aactctaacc agcacgttag
     1021 gtgaaacaat tactgaaatg aaacaaagtg gtgaagaatt cctgaagtca ctagagtcgc
     1081 actcgaaaga attgcataga aatatggaac aaaatacgac taatgtaatt gatatgttca
     1141 gtaagactgg tgaaaagatt aaccatcaac tatctagtaa tgccgataat atgtttgatt
     1201 caatccagac atcatttgat aaggcaagtg cagggctgac ttctcaagtc agagaatcaa
     1261 ttgaaaaatt tgctctatcc atcaacgagc agttacatgc ttttgagcaa gcaactgaac
     1321 gtgagatgaa ccgtgaaatg caatcattag gtaatgctct gctttcaatc agcaaaggtt
     1381 ttgtcggtaa ctatgaaaaa cttattaaag attaccaaat aattatgggg cagttacaag
     1441 cattaatttc tgctaataaa catcgagggt aatcgatcat ggataagatt atagggaaac
     1501 aattacctaa aaaagatcaa gataatgaac actgggtatc catgtcagac ctaatggcag
     1561 ggctgatgat ggtttttatg ttcatatcta ttgcttatat gcactacgta cgtattgaaa
     1621 aagaaaaaat taaagaagtt gccgtagcct acgagaatgc tcagttacag ctttataatg
     1681 ctctggatat tgagtttgca aaggatttgc aagactggga tgcagagatc gataagcaga
     1741 ctctggaggt tcgatttaaa tcaccggatg ttttatttgg cttaggaagc acagagctta
     1801 aaccaaagtt taaactcatt cttgacgact tctttcctcg ctacctaaaa gttcttgata
     1861 attatcaaga acatattact gaagtccgca ttgaaggtca cacaagtact gactggactg
     1921 gaacaacgaa tcctgatatt gcttatttta ataatatggc actatcacaa ggtcgtacac
     1981 gtgcagtatt acaatacgtt tatgatataa aaaatatcgc gacacacaaa caatgggtta
     2041 aaagtaaatt tgccgcagta ggttattcat ctgcacatcc cattcttgat aaaaccggca
     2101 aggaggatcc taatcgctct cgtcgtgtca ccttcaaagt tgtaacaaat gccgagttgc
     2161 agattagaaa gattattcag gagtaagaga tgaaattatc tatcgacatt tcagaactta
     2221 ttcaattagg gaagaaaatg ttaccagaag gagtcgattt ttttctggat gaatccccta
     2281 ttgactttga tccaatagat attgagttat ccacgggtaa agaagttagt atcgaagatc
     2341 ttgaccctgg tagcgggctt atctcttatc atggccgcca ggttctttta tatattaggg
     2401 accattcagg gcgttatgat gcggctatca tcgatggcga aaaaggaaaa cgttttcata
     2461 ttgcctggtg cagaactctt gatgaaatgc gccataaaaa tcgatttgaa aggtatcatg
     2521 caactaaccg catagatggt ttattcgaaa ttgatgatgg ttcaggtcgg agccaggatg
     2581 ttgatttacg agtatgtatg aattgtctgg aacgacttaa ttacaaagga agtattgata
     2641 aacaaaggaa aagagagatt tttaaatcat tctcattaaa tgagtttttt tcagattata
     2701 gtacctgttt tcgtcatatg cctaagggta tctatgacaa aacaaatagt gggtatgtcg
     2761 aaaactggaa agatatatca aaatcaatac gagaaaaggc caagtatact tgtaataatt
     2821 gtggtgtgaa tttatcaacc gccaaaaact tgtgccatgt ccatcataaa aatggcatca
     2881 aatatgataa tcaccatgaa aaccttcttg ttctgtgtaa ggattgccat cgtaaacagc
     2941 ccctccatga aggtatattc gttacccaag ctgagatggc tatcattcaa cgtttacgtt
     3001 cccaacaagg gttattaaaa gccgaatcct ggaatgaaat atatgacctg actgatccat
     3061 cagtacatgg tgatattaat atgatgcaac ataaaggctt tcaacctcct gttcctgggt
     3121 tagatcttca aaactcagaa catgaaatta ttgcaaccgt agaagcagca tggccaggcc
     3181 ttaaaattgc agttaacctt actcccgccg aagtcgaagg atggagaata tataccgtgg
     3241 gtgagctggt taaagaaata caaacaggag cctttacgtc agcaacgttg taaattccga
     3301 aactccgtga aagttaagac tttcacggaa gataaataaa attttcctga tttgtgactc
     3361 aaattacaaa agtcgtttat ggcataactt gtctgttttt ttggtgtgat aggtataaag
     3421 gcatatgcta tggttagcct tatatttaaa actttcctca tacgggtgaa ggttaaagct
     3481 tggtagatag gttacagtca caatgaataa agcaataacg gtgcttgcta taatgatggc
     3541 ttcattttcc gtaaatgcgt atgtattacc ggaaacgccg gtgccattta aaagtggtac
     3601 cggagcaatt gataacgaca ctgtctacat tagtttaggt agcgcaggta cggcatggta
     3661 caagctggag aaacagacca aagataaaaa aatggacagc gttagctgca tttcctggtg
     3721 gaccgagtga tcaagcaact tctgcattta ttgatggcaa tctgtatgtg tttgacggca
     3781 ttggcaaaaa cagcgagggc ttgactcagg tatttaatga cgtacacaaa tacaacccca
     3841 aaaccaatag ctgggttaaa ttgatgtcgc acgcgccgat gggcatggcg ggtcatgtga
     3901 catttgtaca caacggcaag gcttatgtta ctggcggtgt taaccagaat atcttcaatg
     3961 gctattttga agatcttaac gaagctggaa aagattcaac ataccgaacg ccggagaact
     4021 ggacgacgac accacgaagc agtcatttca gtcgggggat gattgtagac aaatttgttg
     4081 acgctatttt gcggttttgg cgcgaaaggt gagcgttcat taatttgatt tatttgcatc
     4141 catcaacgct tttaactcct ccggcgtaaa cggtggtttg cgtcggtgca acatcgcatg
     4201 gcaattaggg cacacaggaa gcaaatcatt aactggatcc agctggtaat cctctttaat
     4261 ggctgaaagc ggcaccaggt gatgcacatg gataaagcct tctgcgatat caccatagat
     4321 cttcaccaga tcaacgccgc atacgttgca ctggcatcca tgatgttcga ccgctttatc
     4381 tcgggccttt ttatcgcgtt catagcggtt tactgtcacc tttatcgccg caccttcaac
     4441 gtatgtctcc tgcgacggca actcatccgg gaacagaacg tcggctatgg gagccgcagc
     4501 ggctttatcc agatgaatat gaaatccctg ctctatgagt agcttgacac atttagattt
     4561 tattccgccg gtgaaatccg caggggtaaa ttgcgtcccc gtcatcaatg tcgctgcgat
     4621 gccagcaatc gccttcgagg ggtaacgtcg gccatgatat tcaatctcat acaaccgtgc
     4681 aggctggaac tgatgagttt ctcccgcatc ccataaatgg atagcttcga tgatataccg
     4741 ccgcggaaga tgatcgggga gtttattcat ggagaatgtc cttatacgtt ctcagaacag
     4801 gcagcaaaaa aacaaatgag atcaaggtaa tatatcttca cttgcaggct tgaacagcag
     4861 aaaatttgcg agcatgatcg caattatttc actgccaggg atatcaatgg cttccagtaa
     4921 atcgctacag caggcaattg ccaatataaa aatctggcac aaggatgaac agcgcgcgcc
     4981 gcataagcca ttgttattgc tatacatatt agcgggatac ctgaatggac atccgcgcct
     5041 tttcgattat ggctcggaaa tctacgaacc tctgcacagt ttactggaac gttttgggcc
     5101 acagcgttca cagtaccgac ctgatatgcc gttctggcga ttacaaggcg acggattctg
     5161 gcaattacac aatgccgagc tttgctcaac agcagggagc agccgacaac caccagtgaa
     5221 ggaactgact gaataccacg ttgcaggcgg tttcgatgaa cagcactacg ccctggtaac
     5281 tggcaataaa aaactcatca atactctggc gcaacagata ctcgaagcgc acttcaccga
     5341 gagtattcag gaagaaattg ctgacgagct gggttttgat ctccagcaaa tccgtaaaca
     5401 gcgcgacccg ttatttcgca aaaatgtatt acgcgcttat aaataccagt gcgcgatatg
     5461 tggctttaat atgcgacacg acgacaccac cgtcgcgttg gaagcggcgc atataaaatg
     5521 gaaacagcac ggcggtcctt gtgaaattcc gaatggcctg gcgctttgcg caattcacca
     5581 taaagccttt gataaaggat cgattggcct ggatgaaaat atgcgggtac tggtctcgga
     5641 tgcggtaaac ggcggaggga tcgttgagag attattctgg gattttgacg ggaaaactat
     5701 tgcgttgcca caggtgcgta aaaattaccc ttttgagggg tttgtggagt ggcatcgaaa
     5761 agaggttttt agggggtgag gttgataact atcgcattat gttcaatatt gctgacataa
     5821 tcagtgcagg atcttaccgc tagtttacaa ctcgccttcg atagcggcgc aaaacgagtt
     5881 ctgttgccga tatcctcggc tatggatatt ccaacagttc cggcagagtt gtttaccaag
     5941 tttcaggtaa gtttttactc agaatcggtt gatgcggttt ataaggcgct ggatgtgaat
     6001 taacacattc ctcagcttat ttacaaaagg attattccca tttgatataa tatcagtgcc
     6061 aatttacttt tatcaatgga atatatctcg gctaaataac aataaaactt atcgctaatg
     6121 gattaaataa acaattttct aattcatttc accacctttg atatagaaat tgatggtgtt
     6181 gtcgctgcca ttgcttatgg tgatgataaa atccacgttt ctggatcgtt gcgtagaaaa
     6241 tcatcactgc cttgatattt tgatgcgata taatcatacc gttc
//
LOCUS       DACBVI010000210         6240 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.212, whole genome shotgun sequence.
ACCESSION   DACBVI010000210 DACBVI010000000
VERSION     DACBVI010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.212"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            430..1524
                     /gene="ompC"
                     /locus_tag="IU325_21080"
     CDS             430..1524
                     /gene="ompC"
                     /locus_tag="IU325_21080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAP5811490.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEIYNKDGNKLDLYGKVDGLHYF
                     SDNDSKDGDKTYMRLGFKGETQVTDQLTGYGQWEYQIQGNEPESDNSSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYDSDNFMQQRGNGFATYRNTDFFGL
                     VDGLDFAVQYQGKNGSAHGEGMTTNGRDDVFEQNGDGVGGSITYNYEGFGIGAAVSSS
                     KRTWDQNNTGLIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSLAYLQSKGKNLGRGYDDEDILKYVDVGATYYFNKNMSTYVDY
                     KINLLDDNRFTRDAGINTDDIVALGLVYQF"
     gene            1636..2691
                     /gene="apbE"
                     /locus_tag="IU325_21085"
     CDS             1636..2691
                     /gene="apbE"
                     /locus_tag="IU325_21085"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HAP5811491.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKTQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LSVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2765..3829
                     /gene="ada"
                     /locus_tag="IU325_21090"
     CDS             2765..3829
                     /gene="ada"
                     /locus_tag="IU325_21090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HAP5811492.1"
                     /translation="MKNATRLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYTDASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRDSLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVRDVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3829..4479
                     /gene="alkB"
                     /locus_tag="IU325_21095"
     CDS             3829..4479
                     /gene="alkB"
                     /locus_tag="IU325_21095"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HAP5811493.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4555..6198
                     /gene="yojI"
                     /locus_tag="IU325_21100"
     CDS             4555..6198
                     /gene="yojI"
                     /locus_tag="IU325_21100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HAP5811494.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFILVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGNPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLIEKWLVQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGLLSELTGEERDAASRDAVARTA"
BASE COUNT         1497 a         1520 c         1682 g         1541 t
ORIGIN      
        1 gtaaaaaaaa ccggatgcga ggcatccggt tgaaataggg gtaaacagac attcagaaat
       61 gaatgacggt aataaataaa gttaatgatg atagcgggag ttattctagt tgcgagtgaa
      121 ggttttgttt tgacattcag tgctgtcaaa tacttaagaa taagttattg attttaccct
      181 tgaattatta ttgcttgatg ttaggtgctt atttcgccat tccgcaataa tcttaaaaag
      241 ttcccttgta tttacatttt gaaacatcta tagcgataaa tgaaacatct taaaagtttt
      301 agtatcatat tcgtgttgga ttattctgca tttttgggga gaatggacct gccgactgat
      361 taatgagggt taatcagtat gcagtggcat aaaaaagcaa ataaaggcat ataacagagg
      421 gttaataaca tgaaagttaa agtactgtcc ctcctggtcc cagctctgct ggtagcaggc
      481 gcagcaaacg ctgctgaaat ttacaacaaa gacggcaaca aattagatct gtacggtaaa
      541 gttgacggcc tgcactattt ctccgataac gactctaaag atggcgataa gacctacatg
      601 cgtcttggct tcaaaggtga aactcaggtt actgatcaac tgaccggtta tggtcagtgg
      661 gaatatcaga ttcaaggtaa cgaacctgaa agtgacaata gttcctggac ccgtgtggca
      721 ttcgctggtc tgaaattcca ggacgttggt tcttttgact atggtcgtaa ctacggcgtt
      781 gtttacgatg ttacttcctg gaccgacgta ctgccagaat tcggtggtga tacctacgat
      841 tctgataact tcatgcagca gcgtggtaac ggtttcgcga cctaccgtaa caccgacttc
      901 ttcggtctgg ttgacggtct tgattttgct gttcagtatc agggtaaaaa cggtagtgct
      961 catggcgaag gtatgaccac taatggccgt gatgatgtat ttgagcagaa cggtgacggt
     1021 gttggtggtt ctattactta caattatgaa ggctttggta tcggtgctgc agtttccagc
     1081 tccaaacgta cctgggatca gaacaacact ggcctgatcg gtactggcga ccgtgctgaa
     1141 acctacactg gtggtctgaa atacgacgct aacaacatct acctggctgc tcagtacacc
     1201 cagacctaca acgcaactcg cgtaggttcc ctgggttggg cgaacaaagc acagaacttc
     1261 gaagctgttg ctcagtacca gttcgacttc ggtctgcgtc cgtccctggc ttacctgcag
     1321 tctaaaggta aaaacctggg tcgtggctac gacgacgaag atatcctgaa atatgttgat
     1381 gttggtgcga cctactactt caacaaaaac atgtccacct acgttgacta caaaatcaac
     1441 ctgctggacg acaaccgctt cacccgtgac gctggcatca acactgatga catcgtagct
     1501 ctgggtctgg tttaccagtt ctaatctcga ttgatatcga acaaagggcc tgcgggccct
     1561 tttttcattg ttttcagcgt acaaacacag ttttttggtg tactcttgcg accgttcgca
     1621 tgaggataat cacgtatgga aataagcttt acccgcgtgg cactgctggc tgccgcgctc
     1681 ttctttgttg gttgcgatca aaaaacacaa cccgccaaaa cccacgctac tgaagttacc
     1741 gttcttgaag gcaaaaccat gggtaccttc tggcgtgcca gcattccggg cattgacgcc
     1801 aaacgcagtg ccgaacttaa agaaaagatt cagacccagc tggacgccga cgatcagctg
     1861 ctttcgacct ataaaaaaga ttccgcgctg atgcgattta acgactcgca aagtttatcg
     1921 ccgtggccgg taagtgaagc gatggccgat atcgtcacca cctcgctgcg cattggcgca
     1981 aggaccgatg gtgcgatgga tataaccgtc gggccgctgg tgaatctgtg gggctttggc
     2041 ccggaacaac agccggttca aattccgagc caggaacaga tcgatgcgat gaaagccaaa
     2101 accggcttac agcacctgtc ggtcattaat cagtcgcatc agcaatatct gcaaaaagac
     2161 ctgccggatt tatatgtcga tctctccact gtcggtgaag gttatgcggc ggatcacctg
     2221 gcacgcttga tggagcagga aggtatttct cgctacctgg tgtcggtggg cggggcgctg
     2281 aacagccgtg gtatgaacgg tgaaggccag ccgtggcgag tggcgattca aaaaccaacc
     2341 gataaagaaa acgcagttca ggcggtggtg gatatcaacg gccacggtat cagcacctcc
     2401 ggcagctacc gcaactatta cgaactggac ggcaaacgcc tttctcatgt tatcgatccg
     2461 caaaccggac gacctatcga gcataatctg gtatccgtga cggtgattgc accgacggcg
     2521 ctggaagccg acgcctggga tacaggtttg atggtgctcg ggccagagaa agccaaagag
     2581 gttgttcgcc gggaagggct ggcggtttat atgatcacca aagagggcga tagctttaaa
     2641 acctggatgt cgccgcagtt taaaagcttc ctggtgagcg aaaaaaatta aagcgcaaga
     2701 ttgttggttt ttgtgtgatg gtgaccggac agactaaagg ctatccttaa ccagggagct
     2761 gattatgaaa aacgccacac gcttaactga cgatcaacgc tggcaatctg tcttagcacg
     2821 cgacccgaac gccgacggcg aattcgtttt cgccgtgcgc accacgggta tcttttgccg
     2881 tccgtcttgc cgcgccagac atgccttgcg ggaaaacgtt tctttctata cggatgccag
     2941 cgaggcgctt gccgctggct ttcgcccctg caaacgttgt cagccagata aagccaatgc
     3001 ccagcaacat cgccttgata aaattaccca cgcctgccga ctgctggaac aggaaacgcc
     3061 tgtaacgctg gaagccttag ctgaccaggt ggcgatgagt ccgttccatt tgcatcgatt
     3121 gtttaaagcg actaccggaa tgacgccgaa agcctggcaa caggcatggc gtgctcgccg
     3181 tttacgcgac tcgctggcga aaggggagag cgtgactaca tctattctta acgctggatt
     3241 ccccgacagc agcagttact accgtaaagc cgacgaaacg ttgggcatga cggctaaaca
     3301 gtttcgtcac ggaggtgaaa atctggctgt gcgttatgcg ctggctgatt gtgaactggg
     3361 ccgttgcctg gtggctgaaa gcgagcgggg gatttgcgcg atattgctgg gcgatgatga
     3421 cgcgacatta atcagcgagt tgcagcagat gtttcccgct gccgacaacg cgcctgccga
     3481 tttgacgttt caacaacatg tgcgtgacgt gatcgccagc ctcaatcaac gcgatacgcc
     3541 gctgacgtta ccgctggata ttcgcggcac tgcttttcag caacaagtct ggcaggcact
     3601 gcgcacgata ccttgcggtg aaaccgtcag ttatcagcaa ctggctaacg ccatcggcaa
     3661 accgaaagcg gtacgggccg ttgccagcgc ctgtgccgcc aacaagctgg ctatcgtaat
     3721 accctgtcat cgagtggtcc gtggtgatgg cacactttca ggttaccgct ggggcgtgtc
     3781 gcgtaaagcg caactgctgc gccgcgaagc tgagaatgag gagaggtaat gttggatctg
     3841 tttgccgatg ctgaaccgtg gcaagagcca ctggcggctg gtgcggtaat tttacggcgt
     3901 tttgctttta atgctgcgga gcaactgatc cgcgatatta atgacgttgc cagccagtcg
     3961 ccgtttcgcc agatggtcac ccccggggga tatactatgt cggtggcgat gaccaactgt
     4021 gggcatctgg gctggacgac ccatcggcaa ggttatctct attcgcccat tgatccgcaa
     4081 acaaataaac cgtggcccgc catgccacaa agctttcatg atttatgtca acgtgcggct
     4141 acggcggcgg gctatccaga tttccagcca gatgcttgtc ttatcaaccg ctacgctcct
     4201 ggcgcgaaac tgtcgctgca tcaggataaa gacgaaccgg atctgcgcgc gccgattgtt
     4261 tctgtttctc tgggcttacc cgcgattttt caatttggcg gcctgaaacg aaatgatccg
     4321 ctaaaacgtt tgttgttgga acatggcgat gtggtggtat ggggcggtga atcgcggctg
     4381 ttttatcatg ggattcaacc gttgaaagcg gggtttcatc cactcaccac cgactgccgc
     4441 tacaacctga cattccgtca ggcaggtaaa aaagaataaa aataagaatt attattgctg
     4501 tgcgcgcgaa gatgtttaaa ctgcgggctg taattcattg tccgggtttt ctgcatggaa
     4561 cttcttgtac ttgtctggcg gcagtatcgc tggccattta tcagtgtgat ggcgctaagc
     4621 ctcgccagtg cggcattagg cattggctta attgctttta tcaatcagcg ccttatcgaa
     4681 acggcggata ccagtctgct ggtgttgccg gagtttctgg ggttattgct gctgttgatg
     4741 gccgtcactc tcggatcgca actggcgctc accactttgg ggcatcactt cgtttaccga
     4801 ctgcgtagtg aatttatcaa gcgaattctc gatacccacg tcgagcgcat tgaacaactt
     4861 ggtagcgcct cgttgttggc ggggttaacc agcgatgtgc gcaatatcac cattgctttt
     4921 gtgcgtctgc cggaactggt gcaggggatc atcctcacta tcggttccgc ggcgtacttg
     4981 tggatgctgt cgggcaaaat gttgctggta acggctatct ggatggcagt caccatctgg
     5041 ggcggtttta tactggtggc gcgggtgtac aaacatatgg cgaccctgcg tgaaaccgaa
     5101 gacaagctgt acacggattt tcaaaccgta ctcgaagggc gcaaagagct gactctgaac
     5161 cgggaacgcg ccgagtatgt gtttaacaac ctctacattc ctgatgcgca agagtatcgc
     5221 catcatatta tccgcgcaga caccttccat cttagtgccg tgaactggtc aaacatcatg
     5281 atgctgggcg caatcggtct ggtgttctgg atggcgaaca gtctcggttg ggctgatacc
     5341 aacgttgccg cgacctattc gttgacgctg ttattcctgc gtacgccgct gctttcggcg
     5401 gttggtgcat taccgacgct gctgacggcg caggtggcgt ttaacaagct gaacaaattc
     5461 gcactcgcgc ctttcaaagc agagtttccg cgcccacagg cgtttcccaa ctggcaaacg
     5521 ctggagctgc gtaacgtgac gtttgcttat caggataacg cgttttccgt tgggccgatt
     5581 aacctcacca tcaaacgtgg cgagctgctg tttctgattg gcggcaacgg tagcggaaaa
     5641 tcgacgctgg cgatgttgct gacgggcttg tatcagccac aaagcggcga aatcttgctg
     5701 gatggcaacc ctgtcagcgg cgaacaaccg gaagattatc gcaaactgtt ttcggcagtg
     5761 tttaccgatg tctggctgtt cgatcaactg ttggggccgg agggtcaacc cgctaacccg
     5821 caactgattg agaagtggct ggtgcagctg aaaatggctc ataagcttga gttaagcaac
     5881 gggcgtattg ttaacctgaa gttatcaaaa gggcagaaaa aacgcgtcgc gctgttgctg
     5941 gcgctggcag aagaacgcga tattatcctg ctggatgaat gggcggcgga tcaggatcca
     6001 cacttccgtc gtgagtttta tcaggtgttg ctgccgctga tgcaggagat gggtaaaact
     6061 attttcgcca tcagtcatga tgatcattac tttatccacg ccgaccgcct gctggaaatg
     6121 cgcaacgggc tacttagcga gctgacaggc gaagagcgcg atgctgcttc gcgtgatgcc
     6181 gttgcccgga cggcataata tttcgacgct gacctggcgg actgctgctg ccaggtcaat
//
LOCUS       DACBVI010000211         6219 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.213, whole genome shotgun sequence.
ACCESSION   DACBVI010000211 DACBVI010000000
VERSION     DACBVI010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.213"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..664)
                     /locus_tag="IU325_21105"
     CDS             complement(<1..664)
                     /locus_tag="IU325_21105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003862286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HAP5811495.1"
                     /translation="MDFSEYQTEIIEDIKSCLEGLQVQPILFMGAGVSQRYLKAPDWE
                     NLLKYLAETCPLITRPYGYYSQTRNDNKPMIASDFSESYAEWAWDVGTSRYPEQYFNG
                     KSSKDIYIKHEIATLLNNLSDSVDFSAMEYTEEIEKLKKVRPHAIITTNYDDLLEKIF
                     DNYQPIVGHNVVTVNYTTYGEIMKIHGSSKEPESIIITNEDYVEFYKRKKYISAKLLT
                     YFV"
     gene            complement(879..1911)
                     /locus_tag="IU325_21110"
                     /pseudo
     CDS             complement(879..1911)
                     /locus_tag="IU325_21110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567368.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            complement(2278..3177)
                     /locus_tag="IU325_21115"
     CDS             complement(2278..3177)
                     /locus_tag="IU325_21115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014831949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP5811496.1"
                     /translation="MNNALYNQIRIFQSIAREGNISAAARKLEITPPSVSNALKLLEE
                     YIGHPLFVRTTRRIELTETGQLLLEQTAAAVESLENSLESIRDQHQEPSGMVRITLSR
                     FAYLLILKPAMAEFCQQYPGIQLEISVYDGTVNIIKERFDLGIRFGDILEGGVVARPL
                     MKPFREGLYASSAYLSRHGTPEVPADLCHHQLIGYRFITNNRILPLLLNDRGEQLTVE
                     MPGQLISNDIDVMADGIRNGLGIGRLFEPVWQLQPDRERFIPVMERYWKTYPPVYLYY
                     PKNAGKTKRVKALIDFLISTTGR"
     gene            3316..3624
                     /locus_tag="IU325_21120"
     CDS             3316..3624
                     /locus_tag="IU325_21120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP5811497.1"
                     /translation="MRILCLDIPAPGASLEKYAPHLNAEALHAWGLYKSGFIRDIYFR
                     QDRPGVAIFLECDSVDEAMNVMAEFPLAKAGLLSFECIPLGSFINWENLFADEFKNKE
                     "
     gene            3633..4322
                     /locus_tag="IU325_21125"
     CDS             3633..4322
                     /locus_tag="IU325_21125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP5811498.1"
                     /translation="MKPAYKPVLCVVSSHPIKGASGVPTGFFLAELTHPLKVLEDAGI
                     KTTIASIRGGQPPVDGFDLSDPVNAWYWNETDFQQRLATTPALSELKGADYSAVFFAG
                     GHGTMWDFRDNQDAQRIIREVYESDGIVSAVCHGPAALVDAKLSSGEYLIKGKNVAAF
                     TNKEEEEVHTTDVVPFLLETALREHGALYHEAPNWSENVVTDDRLITGQNPASAHGVG
                     VALLNALRRTA"
     gene            4396..5343
                     /locus_tag="IU325_21130"
     CDS             4396..5343
                     /locus_tag="IU325_21130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP5811499.1"
                     /translation="MKTLPPVALGTWSWGTGFAGGDTVFGNHLSDTQMAEVFTAAMSK
                     GLNLWDTAAVYGMGSSETALGALVRQFPRENIILSTKFTPQIADKQSAQPVSDMLEAS
                     LGRLGVDAIDIYWIHNPLDVEKWTPGLIPLLQSGKVKRVGVSNHNLAQIRRANEILNA
                     SGYSLSAVQNHYSLLYRASEEAGILDYCRQNNITFFAYMVLEQGALSGRYDSNHPMPA
                     GSGRAESYNAVLPQIERLTAAMKKMGAERNASVAQIAIAWAIAKGTLPLVGATKVHHV
                     LDAACASDIQLRDEEIILLEQLAAETRVDTRGAWEKPMV"
     gene            5446..6123
                     /locus_tag="IU325_21135"
                     /pseudo
     CDS             5446..6123
                     /locus_tag="IU325_21135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013814197.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
BASE COUNT         1540 a         1541 c         1475 g         1663 t
ORIGIN      
        1 cgacgaaata tgttaaaagt tttgcactga tatacttttt acgcttataa aactcaacat
       61 agtcttcatt tgttataatg atgctttctg gttctttact actgccgtga atcttcatga
      121 tttcaccgta tgtagtatag ttaacagtca caacattatg gccaactatc ggttggtagt
      181 tatcaaatat cttttcaagc agatcgtcat agtttgttgt aataatggca tggggcctta
      241 ctttttttaa cttttcaatt tcttcagtgt actccattgc tgaaaaatca accgagtctg
      301 aaaggttatt caacaatgtt gcaatctcat gcttgatata tatatcttta gagctttttc
      361 cattgaaata ctgttcagga taacgagatg tccccacatc ccaagcccac tcagcgtatg
      421 actcagaaaa atcacttgct atcattggtt tattgtcatt tcttgtttga ctgtagtaac
      481 catacgggcg agtgattaat gggcaagttt cagcgagata ctttaacagg ttttcccaat
      541 caggagcttt caagtagcgt tgagataccc ctgcacccat aaagagaatt ggctgtacct
      601 gtaaaccttc aagacatgat ttgatatctt caattatttc tgtctgatat tcagagaagt
      661 ccatacttta gcctttagga tgtgagatgc tggagtagta aatcgccgcg gagcggttcg
      721 gggttctgtg acacccgaat tttacctcgg tttcctttgt atataatatc attgaagtta
      781 tttgcatttc tcagaaatct tctctggctt tgattgctat agccgaaaga tagcgcacac
      841 ttcgccgttg gtcagttagt acgaggtttc cgccaagttc accttttacc aaagatggcc
      901 tcagagcttc cctgaagctt tatcatggtt tcaagtaggt catcttcgtt cagtgagcgc
      961 tgagatttgt tgatgctagg cgcgtgccgt ctgcgggaag gctcatcacc agaacaaggt
     1021 aacgatagtg agcgattatt gtcccgtttt ctctggacct gacgggcaac atccaacaca
     1081 tggccaagtc tcttgttatc tacaatggcg acgggctcaa cctcagaaag ccggtcatag
     1141 gtggagtagg gtagcagcac gccatttagt cgcagctcct tatgtccgtc aggataatgc
     1201 cagacatcaa tatacttgcc gatggctctt cgactgtact cgttgtcctc aatcagatag
     1261 agcattttat cgtattgtac agtcagattt tttgataccc ggcgaggctc tcgccaggta
     1321 aattctgcat caaggtcatc gtctaatgcc agcggccggt acgcgttaaa atcatgcaag
     1381 ggttctttgg caaagcgccg gttataatcc gccatgtatt cttctgcaaa tgcattggcc
     1441 gcttctattg tacagatccc cttaagcctg agctccttaa cgagtcggtc ctgaagtgtc
     1501 aaatgagcac gttcaacgcg gccttttgcc tggctggttt cagcacacaa aggagtaata
     1561 ttaaggcagc gcatggccct ggcaaactgg gtatcgccga gaccggtggt ggcgtgctta
     1621 ttattgactc tgaatacgct ggctttatcg ctgtagagga tcatcggttt accgtattca
     1681 gtggtggtcg cagccatcaa tttgtatcag ctcgccagta caaggacgcc ggtaacgagg
     1741 ctgatgaatt ttcggtgcgc gttgtctgcg ggggatccag agtcccgcgt ttatcatgag
     1801 acgtcggatg gtttcctttc caaggaccag accgtgaact tcctccagct tttcccgcgc
     1861 cagcgtgggg ccaaaatcac ggtagtgctc ccggattata ctgagtgcct ccaatgagcc
     1921 acaaatagaa cattagcatt gagcctctat aattttggtg cgcataatgt atattatgtt
     1981 aaatgtggtg ggtttgttct taagttcatg tgttccgctc tttcagtagc ttaactgatg
     2041 aatcagtcag cagctcttgc cgtgcaagct acgcggcggt tcgtaccaga ttggccacct
     2101 ccatctcctg gttcgctttc ccctcccccc ggtaaacctc acccaacgtt aattaatgat
     2161 gtcagtatgt gggcgctatc cttgtatgta aaatccactg acgattacgc ccatctgtga
     2221 aaaaaaactc acaatgccat tcgatgcatc tccatactga acgaggcgtt atcgtcttta
     2281 tcgccccgta gtggatatca gaaaatcaat cagggccttc actcttttcg ttttacccgc
     2341 gtttttgggg taatagagat acactggcgg gtaggttttc cagtatctct ccatcaccgg
     2401 aataaagcgc tccctgtccg gctgtaactg ccagacaggc tcaaacaagc gcccaattcc
     2461 cagcccgtta cggatcccgt ctgccataac gtcaatatcg ttgctgatta actgccccgg
     2521 catctcaacg gtcaactgct caccgcgatc gttcaggagc agcggaagga tgcggttgtt
     2581 ggtgataaaa cggtaaccaa tcagctggtg atgacagaga tctgccggca cttcaggcgt
     2641 tccatgtcgg gagagatacg ctgaggatgc atataatcct tcacgaaacg gtttcattaa
     2701 cggtcgggcc accacccctc cctcaagaat atcgccaaaa cggatcccca gatcaaaacg
     2761 ctctttgatg atattcacgg taccgtcgta gaccgaaatt tctaactgta tacccggata
     2821 ttgctgacag aattctgcca tcgcaggctt aagaattaac aggtaggcaa aacgcgagag
     2881 cgtaattcgc accatcccag agggctcctg atgctggtcg cgaatgcttt caagtgagtt
     2941 ttccagcgac tccaccgccg cagcggtctg ttccagcaat aattgaccgg tttccgtcag
     3001 ctctatacgg cgggtcgtac gcacaaaaag cggatggccg atatactctt ccagcagctt
     3061 tagtgcattg ctgacggagg gaggcgtaat ttccagtttc cttgcggctg ccgaaatatt
     3121 gccctcccgc gcaatgctct ggaagatgcg aatctggtta taaagagcgt tattcataat
     3181 gttttcctaa gcaattctcg cctgattatt aagtacagtc taaaaatgct taagtcaatg
     3241 accctctaat cttcctgaaa tacgcctctt atactttagt ctctttctac tacgacctga
     3301 gttcaggaga ctatgatgcg aattttatgt ctggatatcc ctgcccctgg agcatcgctg
     3361 gagaaatatg ctccgcacct caacgctgaa gcgctgcacg cctggggatt gtataaatca
     3421 ggcttcatcc gcgacatcta cttccgtcag gacagacctg gcgtcgctat ttttcttgaa
     3481 tgtgactctg tcgatgaagc gatgaacgtt atggccgaat tcccgttggc aaaagcaggc
     3541 ttactaagct tcgagtgtat accgcttggc tcctttatta actgggaaaa tctcttcgcc
     3601 gatgaattta aaaataaaga gtgaggaccg atatgaaacc tgcatataaa ccagtgctat
     3661 gtgtcgtttc cagccatccg attaaaggcg catccggtgt accaaccggt tttttcctgg
     3721 ccgagctgac tcatccgctg aaagtactgg aagatgccgg aataaaaaca acaatagcca
     3781 gtattcgcgg ggggcagcca ccggtggatg gatttgacct cagcgaccct gttaacgcct
     3841 ggtactggaa cgaaaccgat tttcagcagc gcctggcaac aacacctgcg ttatccgagc
     3901 tgaaaggtgc cgattacagc gcagttttct ttgcaggtgg tcatgggacc atgtgggatt
     3961 tccgtgataa tcaggatgcc cagcgtatta tccgcgaagt gtatgaaagt gatgggattg
     4021 tctcagccgt ctgccacggg cctgcggcat tggttgacgc aaaactcagt agcggcgaat
     4081 acctgataaa agggaaaaat gtggcggcct ttaccaacaa ggaagaggaa gaagttcaca
     4141 caacagatgt ggttcccttc ctgcttgaaa ccgcacttcg cgagcatggt gcgctttatc
     4201 atgaagctcc aaactggtct gaaaacgtgg ttaccgacga tcgccttatc acggggcaga
     4261 atcccgcttc agcacatggg gttggtgtgg cgctgttaaa tgcactccgc cggacagctt
     4321 aattaattca tcgttactca ccttacttgg ccggtttaag tcagccacga tccggccttt
     4381 tttcaggagc tgaatatgaa aacactacct ccagtcgcac taggtacatg gtcctggggc
     4441 acaggctttg ccggcggcga caccgtcttt ggtaatcatc tttctgatac tcagatggca
     4501 gaagtgttca ctgcggccat gagtaagggc ctcaatctct gggataccgc agcagtatat
     4561 ggtatgggga gttccgagac tgcgctggga gcattagtcc gtcagtttcc ccgcgagaac
     4621 ataattttat ccaccaaatt cacgccgcag attgcggaca agcagtctgc gcagcctgtc
     4681 agcgatatgc ttgaggccag ccttgggcgc ctgggcgtgg acgcgattga tatctactgg
     4741 atccataatc ccctcgacgt cgaaaaatgg acaccaggac tgatcccact tttacaaagc
     4801 ggtaaggtaa aacgcgtagg tgtttccaac cacaatctgg cacaaatcag acgcgccaat
     4861 gaaatcctta acgcttccgg ttattccctc agtgcagtac aaaatcacta cagcctgctc
     4921 tatcgcgctt ctgaagaggc cgggatcctt gattattgcc gacaaaataa catcacgttc
     4981 tttgcttaca tggtcctgga gcaaggcgcg ctcagtggtc gttatgattc aaatcatccc
     5041 atgcctgccg ggagtggccg ggccgaaagt tataacgcag tactgccgca gatagaaaga
     5101 ctaaccgccg ccatgaaaaa aatgggagcc gaaaggaatg ccagcgtggc acagatagcc
     5161 atcgcatggg ctattgcgaa aggtaccctt cccctcgttg gtgcgactaa agtccatcac
     5221 gttctggacg ccgcctgcgc ttcagacatc cagttacgcg atgaggaaat catcctgctg
     5281 gaacaactcg ccgcagagac cagagtggat acacgaggcg cctgggaaaa accgatggtg
     5341 taaagcccgt ccggaacaga ctgtcatgaa tgacacacac gaacgggctg ttccgggtga
     5401 ttctaatctg tatcactttt actgctttgt atgaatggtg agaatatgaa aatcatttgc
     5461 ctcgaagaac attatctcga cagcgagtta gggcgagcgt gtatgcctgt tgcgcttgag
     5521 caggcacctt tccttggcga cagggggaaa accgtcgctg atggtcataa tcctgaccga
     5581 ctccctgctt tcccgcttta ataaactgaa tgtgccactc tatcttcatc ccgggttacc
     5641 gcttaaaagc gtacagcagg cctacttcac gggcttcagc gcagaggtca acgccaggct
     5701 ttctatgttt gcctggggct ggcatcacga agcggggatc catttgttgc ggctgatgtt
     5761 atcgggcgca tttgataaat atcccaacct gcaggtaatc agtggccact ggggggagat
     5821 gttacccttc tggctccagc gtcttgatga cagcctacca ctggctgcaa cgggtctgtc
     5881 acgcacgcta acgagaacct tccaggaaca cgtttacgtt accccgtcat atgctaacac
     5941 tgccgcactt ccagtttatc tacgcgttaa tgggtgctga aaggatcctg ttctccgtcg
     6001 gttatcccta tcagacccct ggacggtgta aaaaccttta tagacagtct gcccgtcaac
     6061 aaggctgaaa aagaggccat cgcgtttcgc aatgcagaac gtttactggg catcacggtt
     6121 tagttcaccg tttcatgatg actgagcaca ttgtgatcag ccgacaatta ttagtttttg
     6181 cctaaaagtg aatgagcctt tactgctcta aaaaaataa
//
LOCUS       DACBVI010000212         6215 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.214, whole genome shotgun sequence.
ACCESSION   DACBVI010000212 DACBVI010000000
VERSION     DACBVI010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.214"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(268..858)
                     /gene="cobO"
                     /locus_tag="IU325_21140"
     CDS             complement(268..858)
                     /gene="cobO"
                     /locus_tag="IU325_21140"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HAP5811500.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGIVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADPSLDMVLLDELTYMVAYDYLPLEEVVKALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(855..1613)
                     /locus_tag="IU325_21145"
     CDS             complement(855..1613)
                     /locus_tag="IU325_21145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HAP5811501.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            1833..2882
                     /gene="sohB"
                     /locus_tag="IU325_21150"
     CDS             1833..2882
                     /gene="sohB"
                     /locus_tag="IU325_21150"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HAP5811502.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNISEQYKEMKEELAAALMDTHQQKQWHKTQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSVDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(2918..3169)
                     /locus_tag="IU325_21155"
     CDS             complement(2918..3169)
                     /locus_tag="IU325_21155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2498 family protein"
                     /protein_id="HAP5811503.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            3549..6146
                     /gene="topA"
                     /locus_tag="IU325_21160"
     CDS             3549..6146
                     /gene="topA"
                     /locus_tag="IU325_21160"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP5811504.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         1606 a         1466 c         1644 g         1499 t
ORIGIN      
        1 tattttatcc atatcctcac agttatgaac aacctgacga ttaactatgg tactgaaact
       61 tcgccagagt tagccgggat aaattatgac gatgctaatg cgttaattac tgaactgata
      121 ttatttattg aagatgttga cgctgagaat catgaagcat atccgggaca tttgaatgag
      181 attacacgta ttctgaattt cgctattgcc gcttgaatat aatcatgatt aaagcacctc
      241 aagaggcttc ttttgaggtg ctttcactta gtagtcaatc cctatctgcg cttttacacc
      301 ggcatcaaac gcatgtttca cggggcgtaa ttcacttacc gtatccgcca gttcaagtat
      361 atcccgatga caaccacgac ccgtaataat gacggtttgc tgatgtggac gttcatttaa
      421 cgccttcacc acttcttcaa gtggcaaata atcatacgcc accatataag tcagctcatc
      481 gagcaaaacc atatccagtg aaggatcggc cagcattcgt ttggcatgtt gccagacttc
      541 acggcaggcc gcggtatcag attcgcgatt ttgtgtatcc caggtaaagc ccgttgccat
      601 cacctgaaac tcaacgccat gtggctccag tagattgcgc tcgccattag gccaggtgcc
      661 tttaataaac tgcacgatac ccactttttt gccatggcct actgcacgag ttgccgtacc
      721 aaatgccgcg gtggttttgc cttttccatt gccggtaaaa acgatgataa taccgcgttc
      781 atcctgggcc tgggccacac gagcatcgac tttttctttc actcgctgct gacgctgttg
      841 gtagcgttca tcactcattg ggaaattcct ggtttacggc ccggttgggc gtcaaaggtc
      901 atgccggttt tacggcggct gtcatcgccc atcagccaga ggtagagcgg catgatatca
      961 gcgggtgttt taagtttctg tggatcttcg gtcgggaagg cgctggcccg cattgcggtg
     1021 cgcgtaccgc ctgggttaat gcagttgacg cgcaggcgct gctgatattc atcggccagt
     1081 acctgcatca tcccttcggt ggcaaatttc gacgctgcat atgcgcccca gttggctcgt
     1141 ccctgacgtc caacgcttga tgaagtaaag accagtgaac cggcgtccga tttgagtaat
     1201 aaaggaagca gtgcctgggt gagcataaag gtggcattaa cgttgacctg catgacgtcc
     1261 tgccagacct gcggattttg ttcgctcatt gggcaaacat cgccgagcaa tccggcatta
     1321 tgcaaaacac catccagacg cggataatta acggcaatgc gctgtgccag ttgttggcaa
     1381 tcttcggacg tgcaggtcag caaatcgaga ataaaccact gtggctgacg tccagtttct
     1441 tcgtttatgt ggctggctac ctgacgtaat ttttcttcat tacggcccaa cagaatcact
     1501 gtcgcaccat agcgtgcata cgtcatcgcg gcttcacggc caataccatc gctggctccc
     1561 gtcaccagga taatgcgatc attgagtaaa tcttgttttg gctggtaatg catggctact
     1621 cctcaacgac gttgtctgtc gcgctgccag tgtactttat gactttgggg ctttatgcct
     1681 gaaacagaag ggcatttcaa tcagcctggg gcgataacac cgcaaaatgg atactttgtc
     1741 atactttcgc tgcaataaca tctctgcgag acggcttaac atgcctgttg taaactgtga
     1801 gccaaagcgt tgtttaacca aggtggggac tcgtggaatt gttgtctgaa tatggtttgt
     1861 ttttggcgaa aatcgttacc gttgtgctag cgattgcggc gattgccgcc attattgtca
     1921 atgttgctca acgtaataaa cgccagcgtg gcgagttacg ggtcaacaat atcagcgaac
     1981 agtataagga gatgaaagaa gaactggctg cggcgctgat ggacactcat cagcaaaaac
     2041 agtggcacaa aacgcagaag aaaaagcaca aacaagaagc gaaagcagca aaagcgaaag
     2101 ccaaactggg cgaggtggca actgacagta aacctcgcgt ctgggtgctg gattttaaag
     2161 gcagcgtgga cgcccatgaa gtgaactcgc tacgtgaaga gataacggcg gtactcgcag
     2221 cattcaaacc gcaggatcag gttgtgctac gtctggaaag ccctggtggc atggtgcatg
     2281 gttacgggct ggcggcttcg cagctgcagc gtctgcgtga taaaaacatt cctttaactg
     2341 ttacggtaga caaagttgct gccagtggcg gttacatgat ggcctgtgtg gcagacaaaa
     2401 ttgtttccgc accgtttgct attgtgggtt ctattggcgt ggtggcgcaa atgcccaact
     2461 ttaaccgctt cctgaaaagc aaagatattg atatcgaact gcacaccgcc gggcagtata
     2521 agcgcacgct gacgttgctg ggtgaaaata ccgaagaagg gcgggagaaa ttccgcgaag
     2581 agctgaacga aacgcatcag ttatttaaag attttgtgaa gcgtatgcgt ccgtctctgg
     2641 atattgaaca ggtggcaacg ggtgaacact ggtacggaca acaggcggta gagaaaggcc
     2701 tggttgatga aatcaacacc agtgatgaag ttattcttag cctgatggaa ggccgtgaag
     2761 tggtcaatgt acgctatatg cagcgtaaac gactcattga ccgattcacc ggcagcgcgg
     2821 cagagagcgc cgatcgattg ttgctacgct ggtggcagcg gggtcaaaag ccattgatgt
     2881 aaaagacaaa cgcgaggcta agacctcgcg ttttgcttta atcaaccagg tgatattttt
     2941 ctgaaagcac atgggccagg tgtttgaaca tattaaacac cgcggtgctt ttggctgttg
     3001 gcaatccttg ttcatctaaa aagtagtcgc cggtaaatac cagcacgcca ttacgctgcg
     3061 tgacgccggt ggcttcaatc cctgcgagcg tatcctcatg ctcacgaatg attttgttgg
     3121 cctctttcaa cagcgtctcg cggtcgatgg gttgtgtctc tttgttcatt atttactcct
     3181 taaacaagga cattagtcta cgccaggcat ggcttgcaga caaatatacc acgctggtgg
     3241 caagagcgcc ttactggcaa ctttggattt tgcatgctaa taaagttgcg tatcggattt
     3301 tatcaggtac agtgtgacgc tttcgtcaat ctggcaatag atttgcttga cattcgacca
     3361 aaattccgtc gtgctatagc gcctgtaggc caagacctgt taactcagtc acctgaattt
     3421 tcgtgaacag agtcacgaca aggggttgat atccgcagag agcgagtcca tatcggtaac
     3481 tcgttgccag tggaaggttt atcaacgtgc gacgcattcc tggaagaatc aaattaggta
     3541 aggtgaatat gggtaaagct cttgtcatcg ttgagtcccc ggcaaaagcc aaaacgatca
     3601 acaagtatct gggtagtgac tacgtggtga aatccagcgt cggtcacatc cgcgatttgc
     3661 cgaccagtgg ctcagctgcc aaaaagagtg ccgactctac ctccaccaag acggctaaaa
     3721 agcctaaaaa ggatgaacgt ggcgctctcg tcaaccgtat gggggttgac ccgtggcaca
     3781 attgggaggc gcactatgaa gtgttgcctg gtaaagagaa ggtcgtctct gaactgaaac
     3841 aactggctga aaaagccgac cacatctatc tcgcaaccga ccttgaccgc gaaggggaag
     3901 ccattgcatg gcacctgcgg gaagtgattg gtggtgatga tgcgcgctat agccgagtgg
     3961 tgtttaacga aattactaaa aacgcgatcc gccaggcatt taacaaacca ggtgagctga
     4021 atattgatcg tgttaatgcc cagcaggcgc gtcgctttat ggaccgcgtg gtggggtata
     4081 tggtttcgcc gctgctgtgg aaaaagatcg ctcgtggtct gtctgccggt cgtgtgcagt
     4141 cggtggcggt ccgcctggtg gtcgagcgtg agcgtgaaat taaagcgttc gtaccggaag
     4201 agttctggga agtcgatgcc agcacgacca cgccatctgg tgaagcgttg gcgttgcagg
     4261 tgactcatca gaacgacaaa ccgttccgtc cggtcaacaa agaacaaact caggctgcgg
     4321 taagtctgct ggaaaaagcg cgctacagcg tgctggaacg tgaagacaaa ccgacaacca
     4381 gtaaacctgg cgctcctttt attacctcta cgctgcaaca agctgccagc acccgtcttg
     4441 gttttggcgt gaaaaaaacc atgatgatgg cgcagcgttt gtatgaagct ggctatatca
     4501 catacatgcg taccgactcc accaacctga gtcaggacgc ggtaaatatg gttcgcggtt
     4561 atatcagcga taattttggt aagaaatatc tgccggaaag tccgaatcag tacgccagca
     4621 aagaaaactc acaggaagcg cacgaagcga ttcgtccttc tgacgtcaat gtgatggcgg
     4681 aatcgctgaa ggatatggaa gcagatgcgc agaaactgta ccagttaatc tggcgtcagt
     4741 tcgttgcctg ccagatgacc ccagcgaaat atgactccac gacgctgacc gttggtgcgg
     4801 gcgatttccg cctgaaagca cgcggtcgta ttttgcgctt tgatggctgg acgaaagtga
     4861 tgcctgcgct gcgtaaaggc gatgaagatc gtatcttacc ggcagtcgat aaaggcgatg
     4921 ctttgacact cgttgaactg acaccagccc agcactttac caagccgcca gcgcgtttca
     4981 gtgaagcatc gttggttaaa gagctggaaa aacgcggcat cggtcgtccg tctacctatg
     5041 cgtcgatcat ttcgaccatt caggatcgtg gctatgtgcg agtagaaaat cgtcgtttct
     5101 acgcggaaaa aatgggtgaa attgtcaccg atcgccttga agaaaatttc cgtgagttaa
     5161 tgaactacga cttcaccgca cagatggaaa acagcctcga ccaggtggca aatcacgaag
     5221 cagagtggaa agctgtactg gatcacttct tctcggattt cacccagcag ttagataaag
     5281 ctgaaaaaga tccggaagag ggggggatgc gcccgaacca gatggttctg accagcattg
     5341 actgcccgac ttgtggtcgc aaaatgggga ttcgcacagc gagcaccggg gtattccttg
     5401 gctgttctgg ctatgcgctg ccgccgaaag agcgttgcaa aaccaccatt aacctggtgc
     5461 cggaaaacga agtgctgaac gtgctggaag gcgaagacgc tgaaaccaac gcgctgcgcg
     5521 caaaacgtcg ttgcccgaaa tgcggcacgg cgatggacag ctatctcatc gatccgaaac
     5581 gtaagttgca tgtctgtggt aataacccaa cctgcgacgg ttacgagatc gaagagggcg
     5641 aattccgcat taaaggttat gacggcccga tcgttgagtg tgaaaaatgt ggctctgaaa
     5701 tgcacctgaa aatggggcgt ttcggtaaat acatggcctg cactaacgaa gagtgtaaaa
     5761 acacgcgtaa gattttacgt aacggcgaag tggcaccacc gaaagaagat ccggtgccat
     5821 taccggagct gccgtgcgaa aaatcagatg cttatttcgt gctgcgtgac ggtgctgccg
     5881 gtgtgttcct ggcggccaat accttcccga aatcgcgtga aacacgtgcg ccactggtgg
     5941 aagagcttta tcgcttccgc gaccgtctgc cggaaaaact gcgttatctg gccgatgcgc
     6001 cacagcagga tccggaaggt aataagacca tggttcgctt tagccgtaaa accaaacagc
     6061 aatatgtctc ttcggaaaaa gacggaaagg cgactggctg gtcagcattt tatgttgatg
     6121 gcaaatgggt tgaaggaaaa aaataacctt taattctgtc aggtttttat aaacaaaggg
     6181 tcgcgaaagc ggcccttttt tattgcatat tattt
//
LOCUS       DACBVI010000213         6146 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.215, whole genome shotgun sequence.
ACCESSION   DACBVI010000213 DACBVI010000000
VERSION     DACBVI010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.215"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            336..917
                     /gene="mdaB"
                     /locus_tag="IU325_21165"
     CDS             336..917
                     /gene="mdaB"
                     /locus_tag="IU325_21165"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HAP5811505.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            948..1262
                     /locus_tag="IU325_21170"
     CDS             948..1262
                     /locus_tag="IU325_21170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="catalyzes the oxidation of menadiol to menadione;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP5811506.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1307..2194)
                     /locus_tag="IU325_21175"
     CDS             complement(1307..2194)
                     /locus_tag="IU325_21175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP5811507.1"
                     /translation="MIQKDKTRVDIYGERFRTRASQLTPGLRAVASYINEHREVVLEQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAMPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(2191..3138)
                     /locus_tag="IU325_21180"
     CDS             complement(2191..3138)
                     /locus_tag="IU325_21180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP5811508.1"
                     /translation="MRLFFSLLILLSFFARATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(3149..4186)
                     /locus_tag="IU325_21185"
     CDS             complement(3149..4186)
                     /locus_tag="IU325_21185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP5811509.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLVLLGGLFLFAVGILIFGLMHGS
                     FSVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCARDISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(4195..5178)
                     /locus_tag="IU325_21190"
     CDS             complement(4195..5178)
                     /locus_tag="IU325_21190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP5811510.1"
                     /translation="MMRTGLVTLIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFEP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AATALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWVTLGTSAWFSLGGFVLAIYLAPSLNMLALG
                     DRIAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(5175..5984)
                     /locus_tag="IU325_21195"
     CDS             complement(5175..5984)
                     /locus_tag="IU325_21195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP5811511.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARILQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINSLTGKPFFMPFRGVEKV"
BASE COUNT         1754 a         1656 c         1407 g         1329 t
ORIGIN      
        1 tccagtcgga gatctggttt tcgataccct ccagtaaatc tatttaaaat aatctgcgta
       61 acacgctgat attttcattt ttagttattc aacaccacaa ttatcaactc gcagaaagtc
      121 cacaccaacg ttgtaaatta accctgtgct agttggaatg atgagaaatt gctatcagta
      181 ttgtcatttt cagaaaatat ttatcctgca tcggtgagtc agagcaagat cagacttttg
      241 ctaaattagc aaaagacttt gcacattttg ctaatttcac cgtaccgctc tgtgacgtac
      301 tatagtcgac aaacgtctca ccttgaggtt aaaaaatgag caacatcctg attatcaacg
      361 gcgcgaaaaa attcgcccac tccaatggtc aactgaacga caccctgacc gaagtcgcgg
      421 atggcacact gcgcgacctc gggcatgatg tccgcatcgt tcgcgccgac agcgactacg
      481 atgtcaaagc ggaagtacaa aactttctct gggctgatgt ggtgatttgg cagatgccag
      541 gctggtggat gggcgcgccg tggacagtga aaaaatacat tgatgatgta ttcaccgaag
      601 gtcacgggac gctgtatgcc agcgatggtc gtacccgcaa agatccgtcg aaaaaatacg
      661 gttccggcgg cctggtacag ggcaaaaaat atatgctttc tctgacctgg aacgcaccaa
      721 tggaagcctt caccgaaaaa gatcagttct tccacggcgt tggcgttgac ggtgtgtatc
      781 tgccgttcca taaagcaaac caattccttg gcatggaacc gctgccgaca tttatcgcta
      841 atgacgtgat aaaaatgcct gatgttcccc gctatactga agaatatcgc aagcatcttg
      901 tggaaatttt tggttaacta gagctcaggc tttagaagga gttaaccatg cttaccgtaa
      961 tcgcagaaat ccgtactcgt cctggtcaac atcaccgtca ggcggtattg gatcagtttg
     1021 ctaaaatcgt tccaaccgta ctgaaagaag aaggttgcca cggctatgcg ccaatggtgg
     1081 attgcgctgc tggcgtgagt ttccagtcta tggcaccgga ttctatcgtg atgattgagc
     1141 agtgggaaag catcgcgcat cttgaagcgc atctgcaaac cccgcacatg aaggcgtaca
     1201 gcgaagccgt aaaaggcgac gtgctggaga tgaatatccg tattctgcaa ccggggattt
     1261 aacattcctg aaaagaggcc ggtgttgcta ccggcctcag gtcagattag ccattctttc
     1321 ttcccgtttt caaaccccga tggaaatcgt taattcgctt aatggtttta accgcacgct
     1381 gcggcatcgc ggaagccacc gaccaaacaa tcatttccag gcacaacaat accgtaccat
     1441 gtagtggcgt tttccccttt tcatccccgc gcggaacatg aatcacgata ctggcgtctt
     1501 tgctaaaacg tgaatccagc gcgtttgtca gcaaaataac agggatcccc agccgcctcg
     1561 cctcacgaag cgttgtcagc ccttcccggt gcgcagattt ttgcgccatc atgatcagaa
     1621 catcgccacg ttgaagcgcg atcagttgct cggcaaggcc aatacccgta cggtttagtg
     1681 ccgtggcagg caaacctatg cggttgaata gtcgtgcggt atagtccgcc aggatacccg
     1741 aagcgccaat gccaaaaata gcgacttgcc gtgcctgcac caacaacgct acagcttgcg
     1801 caagcgcgtg gcggttttca ggctctgaca ggacattaca agtatacaaa tgcccttcaa
     1861 gaacaaagtc gattgcggtg ttaacatcgc tcgtcaagtt actcaccgtc gtagacatct
     1921 tttcgctgga actaagggct ggaccaagcc actgttccag agtgcgtttc agatcacgca
     1981 gcccggcaaa ccccagagcc tgtatagccc tgataactgt ggcatcggaa gtgttcaacg
     2041 tggccgcaat ttccatcgcc gtttgctcga gtaccacttc acgatgctca ttaatgtagc
     2101 ttgcgacagc tcttaaccca ggcgttaact gactcgcgcg agtgcgaaaa cgctcaccat
     2161 aaatatcaac ccgcgttttg tctttctgga tcatttagcc tccggtagag gtcgtcccgc
     2221 aatttgtgat tgtatctgcg cagccatcag ccccagagat gcaaaggagt tagaaatcgc
     2281 ctcttcacga gaaatcagca catagcgccc ggaacgacag gccgtcaaaa actgacacca
     2341 tcccggcatg actttttcca ttgctgccag ctcatcctga ggtttaccac cggtgtcacc
     2401 gcgccaggtt gcaaaaacaa aatcggcatc cagttcaggt aaacgctctg cgctcacatc
     2461 cattcgccca ccttcaggaa tgctctcaat cagcggcgga aagcgaaaac cagcatcgcg
     2521 taatacccgc cccagcgaat gatagctatg catcacatta atttttccct gatttgcctg
     2581 aatcaccgat accgttattt tttggctgtc cagcgtcgcc ttcagcgcat tgatttgtgc
     2641 ctgatagcgg cgttcaagta tcgccagctg cgactgcgtt cctgtaagtt ctgccagctt
     2701 gcggtagatt tctggcgcgc cgcctttgag atgatcaatg ctcaccgtgg gcgcaatttt
     2761 ttccagttgt tcaatgggcg tattacgggt cggttctgtg atgatcaggt caggctttgc
     2821 cgccacaatc gcttcaatat caatatcagc ggtgccaata aaagcgatcg atgaattatc
     2881 aaagtcgaca cctgtcaata acgcgcctga tcgaatgaaa tggctaccgt caggccgggt
     2941 tcgcccgtgg ctggcgacag gaggtacacc gagctcaatc agaggaatag tgatatcaag
     3001 atcgtgtaaa gagacaattc tctttggatg agcaggaaca gtgactttgc gccctaaatc
     3061 atcagtaaag acctgaacag gttctgtcgc gcgggcaaag aatgacaaca gtatcagcag
     3121 agaaaaaaat aaacgcatat aactcccgtt aaaaactgtt cctgcgctgc cagagcagta
     3181 gcaaaaagaa tggtccacca atcaatgaaa taatgattcc ggcaggcagt tgcaaaggca
     3241 aaaaagccaa acgaccaatg ctatccgcca ggatgactaa caacgccccc gatacggcgc
     3301 tccccagcag taatgtcgtc tgcccgccac gtagaataaa acgtgatata tggggtgcta
     3361 tcaatccgac aaaaccgatg ttaccaacac acgaaacgca gacggctgtc aggattatgg
     3421 gtgcggcgac acgtaacaac gccagacgag aactattcac ccctaacccg gttgcaacct
     3481 gatgaccaag cagagcgata tccgccgtcc tggcggtgaa cagcaataaa agagcggctg
     3541 gcaacatcca acaggctgaa ataccgacta acatccagtt agctgcatgc aggcttcccg
     3601 ataaccacat caatgcagtt tgtacatctc gaacatctgc agtggtcata aatacaccga
     3661 taccggcggc gaaaaaccag gaaacaccaa taccaatcag aacaaatcgc gggcgggaaa
     3721 tatcgcgtgc acaaaaaatc acgattaaag ccaccaacaa gccaccagca agaccagcca
     3781 ctggacgcca gaacattccc agcattggaa actggaaaat cagccacagc accgccacgc
     3841 tacatccttc tttgacgccg attaaaccag gatcggcaag gccgttacgc gcaatggatt
     3901 gcatcgcggc ccccgccata cctaacatgg ctccgcatag caatgccatg atgactctcg
     3961 gcaagcgaat atcctgcaca atataacgtg cgtctgtgct tacgttttct ggagcaaaca
     4021 acgctcgtcc tacttccgat gctgggacag aaaaagaacc atgcatcaga ccgaatatca
     4081 gtattccaac cgcaaacaga aacagcccac caagcaacac cagattttta gggcgtacca
     4141 gtgtcgaaaa cttaccaaca cgtagtggac gtagcccgac gttcattatc cgactcattt
     4201 gaacatcctc gtcgccataa tgacaaaaac gggcgcacca actaacgccg tcatgacacc
     4261 ggtcgccagc tcatggggcg tgaaaagcgt ccgggcaata atatctgcca gtaatagcag
     4321 taaggcaccg acacaagctg aaagagggag cagaacgcgc agatccgcag ataccagacg
     4381 cctgacaatc tgcggtacta gcagaccgac aaaaccaatg gggccagcaa tcgatacggc
     4441 tgcgccacag agcaacgcga tcgcaagcag ggtgaatgtt cgcgtgcgga gtaccgagac
     4501 gcccagcccc tgcgctatgc gatcacccag tgccaacatg ttaagagagg gcgcaaggta
     4561 aatggctaac acaaatcccc ccagagaaaa ccaggcggac gtacccagcg tcacccaatc
     4621 ctgccccgct aaatcacctg ctagccatgt ccgcatcgcc agcagtgttt gttcgtcaag
     4681 aatcaggatt gcagccgtta ttgaggacac gaacgccgat agcgccacgc cgcataaagt
     4741 aactttcatt ggcgttaacc ctgaacgccc tgcggaggaa agcagcaata tcaacaggaa
     4801 caataacgcg ccgcccgtag atgccagcag tggccgtcca accggaaagg ccagcccaag
     4861 cgcagtggca gcaacgacag caagagcggc tccggcattt aagcctaaaa tatgtggctc
     4921 ccccaatggg ttacgaatga cagcctgcaa caaagcacca gcaactccaa gagaagcacc
     4981 ggtaagcaat gctgccgcta aacgcggcaa acggagtctt acaattacgt tgtgatcgaa
     5041 gttttgcggt tcaaaatgaa gaaatgcctg caacaccgtt tgtggtgcaa taaatcgggc
     5101 accaatccct ggatatacca cgcatcccac cagcagtaag gcgaggaaaa ttaaagtaac
     5161 cagccctgtg cgcatcatac tttttctaca ccacgaaacg gcatgaagaa cggctttccc
     5221 gtcagagaat ttattgatgc gtgaacatca acgtcaaaga cagcttttac cagctcaggc
     5281 gtgcaatgct ccccctcatt caacacacgc accactttcc cctggcgtaa aaatatcaac
     5341 gtatcaccat aattgacggc aaaattcaga tcatgcaaca ccacgacgac tgtacgcccg
     5401 tgatgacgcg ttaaatcatg taataactca agaatttcaa cctgataacg taaatcaagc
     5461 caggttgtgg gttcatccag caggatataa ggcgtctttt gcgccaacac catcgcaatc
     5521 cagcaacgct ggcgctgccc accagaaagt ttttcaacag gtagatgtgc aaactcctgc
     5581 gtaccggtta atttcagggc ctcttctacc gcctgctcgt cggcgtcgct ccattgacga
     5641 atgaaattct gccagggaaa acgcccacgc gacaccagtt cataaacggt taagccctcc
     5701 ggcaataatg gcgactgcgg caagatccca agtctacggg ataacgcttt cgttggttgc
     5761 tcgtgaatcg ctttaccatc cagcaatatc gagccgccca taggttgcaa gatacgtgca
     5821 atggtgctga gcaacgtcga ctttccacag ccgtttgccc caaccagaac ggtcattttt
     5881 tctgtgggaa cggagaaaga aacaccatca acaatgattt tcttgtgata cccggcagaa
     5941 acattgtcga ggatcagccc ctgccctttt gcgttatgtg ccacgtgtac gccaacttaa
     6001 tcaaagacaa acaaaaataa aactcattct cctttgagag tttgacgcca caaaatgtag
     6061 tagtcaagaa gttaaaagcc atacaaaaac aaggtaaata acaggtgaaa tatttttaaa
     6121 gataaaaata aaaccattaa atatta
//
LOCUS       DACBVI010000214         5932 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.216, whole genome shotgun sequence.
ACCESSION   DACBVI010000214 DACBVI010000000
VERSION     DACBVI010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.216"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..2223)
                     /locus_tag="IU325_21200"
     CDS             complement(<1..2223)
                     /locus_tag="IU325_21200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAP5811512.1"
                     /translation="MNRISRYYFHRSVLSLLIAAMIYAPPGMTAFTSNVIGVVNDETV
                     DGSQRVDERGTTNNAHIINHGNQEVYGGISNGSVIDTGGHQEVSGHGSYQGQANNTVI
                     NGGSQTISEGGISTGTIINDKGTMSVLTNAKADATRIDNGGAMDVAGNATNTIINGGT
                     QNIYNHGIATGTNINSGTQNIKSGGKADTTNISSGSKQVVEKGGTATGSNIRAGGTLI
                     VDTGGIAHGVYLDTGSALVANTGAGTDIDGYQRSSHFTITGGRAEHVVLENTGQLTVV
                     AQTSAVDTIVDAGGKLIVHEEAVAYTTRLNNGGILDVREKGSATGIQQSSQGALVATT
                     RATRVTGTRADGVAFSIEQGAANNILLTNGGVLTVESDTTSAKTQVNAGGREIVKTKA
                     TATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTTINEGGTLTVNDNGKATD
                     IVQNSGAALQTSTANGIEISGTHQYGTFSIASNLATNMLLENGGNLLVLAGTEARDST
                     VDKGGAMQNLGQDSATKVNSGGQYTLGRSKDEFQALARAEDLQVAGGTAIVYAGTLAD
                     ASVSGATGSLSLMTPRDNVTPVKLEGAIRITDSATFTIGNGVDTTLADLTAASRGSVW
                     LNSNNSCAGTSNCEYRVNSLLLNDGDVYLSAPATTNGIYNTLTTSELSGSGNFYLHTN
                     IAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGGDASFTLGNTGGFVD
                     L"
     gene            complement(2295..2414)
                     /locus_tag="IU325_21205"
     CDS             complement(2295..2414)
                     /locus_tag="IU325_21205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP5811513.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(2375..2560)
                     /locus_tag="IU325_21210"
     CDS             complement(2375..2560)
                     /locus_tag="IU325_21210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811514.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     IAFKKCLFLMRDIIISS"
     gene            2661..2834
                     /locus_tag="IU325_21215"
     CDS             2661..2834
                     /locus_tag="IU325_21215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811515.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            2896..3180
                     /locus_tag="IU325_21220"
     CDS             2896..3180
                     /locus_tag="IU325_21220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HAP5811516.1"
                     /translation="MADMNRTTKGALLGAGVGLLTGNGVNGVLKGAAVGAGVGAVTEK
                     GRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVIGGTGGAILGKMK"
     gene            3184..3519
                     /locus_tag="IU325_21225"
     CDS             3184..3519
                     /locus_tag="IU325_21225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811517.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(3575..5818)
                     /locus_tag="IU325_21230"
     CDS             complement(3575..5818)
                     /locus_tag="IU325_21230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAP5811518.1"
                     /translation="MNGDNSVETRGAYSAGLLSQVNDSGIVENNTRLETTDKTNIVTY
                     GENAVGVLACSSPGESRTCVDAVDDEVSDSNSYEVISRADLKMNGGSITTNGTNSYGA
                     YANGEKAYINLDYVALETGEHGSYAVAIRQGNIDIKNSSITTKGTKAPIAKIYNGGEL
                     FFSNVTAVSEQDKGISIDASNIDSQAKIALSSVELSSALDSIDVNKTTTDVSILNRSI
                     ITPGNNILVNNTGGGLNIISSDSTLNGATKLVSGTTTLKLSENTIWNMKDDSVVIHLT
                     NSDSIINLSYDDGQTFTQGKTLTVKGNYVGNNGQLNIRTVLGDDKSATDRLIVEGNTS
                     GSTTVYVKNAGGSGAATLNGIELITVNGDESPADAFRQGDARIAAGAFEYQLKQQGKN
                     WYLTSYQSVNEEDNSSEGNSESTETPTPVLRPEAGSYVANLAAANTLFVMRLNDRAGE
                     TRYIDPVTEQERSSRLWLRQVGGHNAWRDSNGQLRTTSHRYVSQLGADLLTGGFTDSD
                     SWRLGVMAGYARDYNSTHSSVSDYRSKGSVRGYSAGLYATWFADDISKKGAYIDAWAQ
                     YSWFKNSVKGDELAYESYSAKGATVSLEAGYGFALNKSFGLEAAKYTWIFQPQAQAIW
                     MGVDHNAHTEANGSRIENDANNNIQTRLGFRTFIRTQEKNSGPHGDDFEPFVEMNWIH
                     NSKDFAVSMNGVKVEQDGARNLGEIKLGVNGNLNSAASVWGNVGVQLGDNGYNDTAMM
                     VGLKYKF"
BASE COUNT         1482 a         1483 c         1285 g         1682 t
ORIGIN      
        1 aagatcaacg aaaccaccgg tattgcccag cgtaaacgac gcatcccctc cccctgtttt
       61 caccagcgtc atcgcatcgt cagactgagg gctgacgccg gtatcctgaa caaagatttt
      121 aaaattacca gtggcattgt tgttgacgac cagttgatcg ccccgggagc ctgcaatgtt
      181 ggtatgcagg tagaaattac cgctgccgga aagttcactg gtggtcagcg tattgtagat
      241 accgttagtt gtggcaggcg ctgataaata aacatcaccg tcgttgagta gcaaactgtt
      301 tactctatat tcgcagttgc tggtgcctgc acaggaatta ttgctgttaa gccagacact
      361 gccccggctg gcagccgtca ggtcggcaag cgtcgtatca acgccattgc cgatagtgaa
      421 tgtcgcgcta tcggtaatcc ggatcgcccc ttcgagttta actggcgtaa cattatcccg
      481 tggcgtcatt aacgacaggc ttcctgtcgc gccactgacc gatgcatccg ccagcgtacc
      541 tgcgtagacg attgctgtcc cgccagcaac ctggagatct tctgcccggg ccagagcctg
      601 aaactcatct tttgaccgcc caagggtata ttgcccacca gagttaacct ttgtggcgga
      661 gtcctgaccc aggttttgca ttgcaccacc cttgtcaacc gtggagtcac gggcttcggt
      721 acctgctaat accaataaat taccgccatt ttccagcaac atattggtcg ctaaattgct
      781 ggcaatggaa aaggtgccgt actggtgcgt accgctgatt tcaataccgt tagccgtgct
      841 cgtctggaga gcggcaccgc tgttctggac gatatctgtc gctttgccat tatcgttgac
      901 tgtcagcgtt ccgccttcat tgatcgttgt ttttattgcc tctccgttag ctgaaactgt
      961 ttgtattccg ccgtcgttaa ttgttgtctc atttgccaca ccctcgacga tttgttcacc
     1021 gccagtgagc gtcgtgcctg tcgcagtggc ttttgttttg acgatctccc gaccgcccgc
     1081 attgacctgt gttttggcgg aagtggtgtc tgactccacg gttaacacgc cgccatttgt
     1141 cagcaggata ttgttcgccg caccctgctc gatgctgaac gcgacgccat cagcgcgtgt
     1201 ccctgtgacc cgcgtcgccc tggtggttgc aaccaacgcg ccctgactac tctgctgtat
     1261 ccccgttgcg ctgcctttct cccgcacatc gagtatgccg ccattgttaa gtctggtggt
     1321 atatgctacg gcctcctcgt ggacaatcag cttaccgcca gcatcaacga tggtatctac
     1381 cgccgaggtt tgagctacga ccgtcagttg gccggtattt tccagcacaa catgctcagc
     1441 cctgcctccg gtaatagtga agtgagatga cctctggtat ccatcaatat cagtccccgc
     1501 gcccgtgttg gcaactaaag cactccccgt gtccaggtaa accccatgtg cgataccgcc
     1561 ggtatcgaca atcagcgtgc ctccggcacg aatattgctg ccagttgccg tacctccttt
     1621 ctcaacaacc tgcttgctcc cggaagagat atttgttgtg tcagctttcc cgccgctctt
     1681 gatattttgc gtgccactgt tgatattagt tcccgtagca ataccatgat tataaatatt
     1741 ctgtgtaccg ccattaatta tggtattagt cgcgttcccg gcaacatcca tggccccgcc
     1801 gttatctatt cgggtcgcat cagctttagc attcgttaaa actgacattg ttcctttatc
     1861 gttaattatc gtccctgtag aaattcctcc ctcactaatt gtctggctgc caccgttaat
     1921 gactgtatta ttagcctgcc cctgataaga gccatgacca gatacctctt gatgtccacc
     1981 agtatcaata acacttccat tagaaatccc gccatatacc tcctgattgc cgtgattgat
     2041 gatatgagca ttatttgttg tacctcgttc atctactctt tggctgccgt ctacagtctc
     2101 atcatttaca acgccaataa cattagaagt gaaggccgtc atcccaggcg gggcatatat
     2161 catggcagct atcaataagg aaagtactga gcggtgaaaa taatagcgac tgattctgtt
     2221 cataaatttc atcctctgaa aaatgaatac tgagtactga gtagcgtcta agtaacctaa
     2281 gctcggctgc aacatcagcc cgcaggcacc agaccagggg attcatcctg aagagacagc
     2341 gcaggtgtat tgtgttcacc gctcaccaaa gacatcatga tgaaatgatg atatcccgca
     2401 taagaaagag gcatttttta aacgcaatgc gctgaagtgt agttggataa aaaatcactc
     2461 ttttcagata atactgtcgt acgcttatct ttcgacaatg aggccgtcgg caaaataaaa
     2521 tgactaatgt aatcgttgct gatgaatatc ttctgctcac ataaaaatca cacaataata
     2581 ttgataaatc agattatctt acctttacaa cattgcgtta acagtcaatg acacttcttg
     2641 aataaaaata ttgtcaatca atgagttatt cctcatttaa gattattctc attcacgtaa
     2701 aaaacataat tcctatcatc acagccacgc tcattttgaa ttatctgaat aatagcgagc
     2761 gttctttagt taagcagatc ttaattgaag acgaaattat tgtgtgtgca acttacttaa
     2821 taccggacat ctaagttgaa gaaaaaaata cttgccttcg ggcttatctc agcattgttc
     2881 tgctccacgc cagcgatggc ggatatgaac cgcaccacga aaggtgcatt gctaggcgca
     2941 ggcgttggtt tacttactgg caatggtgtt aatggcgtac tgaaaggtgc agctgttggc
     3001 gctggtgttg gtgcagtaac agaaaaaggc cgcgacggta aaaatgcgcg taaaggtgca
     3061 aaagtgggtg ccgccgttgg tgctgtcact ggcgttctga ccggcaatgg cctcgaaggg
     3121 gcgattaaag gtgccgtgat tggcggtacg ggtggtgcaa ttctgggtaa aatgaaataa
     3181 ggaatgatga tgaaaaaatt tgctttactg gcgggtctgt ttgttttcgc accaatgacc
     3241 tgggcgcagg actacaatat taaaaacggt ttgccgtcag aaacctatat tacttgcgca
     3301 gaagccaacg aaatggcaaa aactgacagc gcacaggttg cagaaattgt tgcggtaatg
     3361 ggtaatgcca gcgttgccag ccgtgattta aaaattgagc aatctccgga actaagcgcc
     3421 aaagtggtag aaaaactgaa tcaggtttgc gctaaagacc cgcaaatgct gttgatcacg
     3481 gcaattgatg acactatgcg tgctatcggc aaaaaataat atttaatatg ttaccgcgtc
     3541 gatgaattcg acgcggtatt ttagctaacg gggatcagaa cttatatttc aggcccacca
     3601 tcattgcggt gtcattgtag ccattatcac ccagctgcac gcccacatta ccccagacgc
     3661 tggccgccga attcagattg ccatttacgc caagtttaat ttcccccaga ttacgcgccc
     3721 cgtcttgttc aactttcaca ccgttcattg agacagcaaa atctttactg ttatggatcc
     3781 agttcatttc aacaaaaggt tcaaagtcgt caccgtgcgg accgctgttt ttctcctgag
     3841 tacgaataaa ggtgcggaag ccgagtcggg tctggatgtt gttatttgcg tcattctcaa
     3901 tacgtgagcc attggcttcc gtgtgcgcat tatgatcgac gcccatccag atagcctgtg
     3961 cctgtggctg gaagatccac gtatatttcg ccgcttccag accaaaggat ttattcaggg
     4021 caaaaccgta acccgcttcc agcgagacgg ttgcaccttt cgcgctatag gattcatagg
     4081 ctaactcatc ccctttcacc gagtttttaa accagctata ttgcgcccag gcgtcaatgt
     4141 atgcgccttt tttactgatg tcatcggcaa accaggtggc atacagccct gcgctatagc
     4201 ctctgacact ccctttcgaa cgataatccg acacgctgga atgagttgag ttgtagtcgc
     4261 gggcataacc agccatcact cccaaacgcc aactgtcact gtcggtaaaa ccaccggtta
     4321 acagatcggc ccctaactgc gagacgtagc gatgcgaggt cgttctcaac tgtccgttgc
     4381 tgtcacgcca ggcattatgc ccgccaactt gacgtagcca aagccggctt gaacgctcct
     4441 gttcagttac aggatcgatg taacgcgttt cacctgcacg atcgttcaga cgcataacaa
     4501 aaagagtgtt agcggctgcc aggttggcga cgtaacttcc agcttcgggg cgcaggacgg
     4561 gggtaggcgt ttctgttgat tctgaatttc cctctgagct gttatcctcc tcattcactg
     4621 actgataact tgtcaaatac cagtttttgc cttgttgttt tagttgatat tcgaaagctc
     4681 cagctgcaat ccttgcatca ccttgtctga aggcatctgc tggagattca tcgccattca
     4741 cagttatgag ttcgatccca tttagcgtgg ccgcgccgct tcctccagca tttttcacat
     4801 agacggtagt tgaccccgaa gtattaccct caacaataag tctgtcagta gccgatttat
     4861 catcacctaa tacggtgcgg atattaagct gaccattatt accgacataa ttacctttta
     4921 cggttaacgt ttttccctgg gtaaatgttt gaccatcatc atacgaaaga ttgataatac
     4981 tgtctgaatt agtcagatgg ataacaacgg agtcatcttt catattccag attgtgtttt
     5041 ctgaaagctt cagtgtagtt gtgccgctaa cgagtttagt cgctccattt agagtagagt
     5101 cggacgaaat tatgtttaag ccacctccag tattattaac cagaatatta ttaccaggcg
     5161 tgataatact tcgattaagg atgcttacat ccgttgtagt tttgttaaca tcaatactat
     5221 ccaaagcact tgaaagttcg acacttgata gtgctatttt ggcttgagaa tcgatatttg
     5281 atgcatcaat tgatattcct ttatcttgtt ctgataccgc ggtgacattg gaaaaaaata
     5341 actctccacc attgtatatt tttgcaatgg gggctttagt gccttttgtt gtaatagaac
     5401 tattttttat atcaatgtta ccttgtcgaa tagcaactgc ataacttcca tgctccccgg
     5461 tttcaagtgc cacataatct aaattaatat atgctttttc cccattagca taagcaccat
     5521 agctattagt gccattagtt gttatggaac caccattcat ttttaaatca gcacggctaa
     5581 taacttcgta actgttagaa tcactaactt catcatctac agcatcgaca catgttcgag
     5641 actctccagg acttgaacat gcaagaacac ctactgcatt ttccccatag gtaacaatgt
     5701 tcgttttatc tgtggtttca agacgggtgt tattttccac aatcccagaa tcattaacct
     5761 gacttaaaag tcccgctgaa tatgcccccc tcgtctcgac tgaattatca ccattcataa
     5821 cgatggtgcc accaaattca ctgactactg cgctggctgt tatccctcca ttaacatctt
     5881 ggttatttcc tttgtcagat acgcttccag aggtcttaat atcgctatcg ct
//
LOCUS       DACBVI010000215         5863 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.217, whole genome shotgun sequence.
ACCESSION   DACBVI010000215 DACBVI010000000
VERSION     DACBVI010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.217"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(83..940)
                     /gene="sitD"
                     /locus_tag="IU325_21235"
     CDS             complement(83..940)
                     /gene="sitD"
                     /locus_tag="IU325_21235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HAP5811519.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFIAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDIHLDHILFGDMLGVTIGDIIQTMIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HITLLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVISFNARTKGNA
                     HRQDLLSPN"
     gene            complement(937..1794)
                     /gene="sitC"
                     /locus_tag="IU325_21240"
     CDS             complement(937..1794)
                     /gene="sitC"
                     /locus_tag="IU325_21240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HAP5811520.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1791..2618)
                     /gene="sitB"
                     /locus_tag="IU325_21245"
     CDS             complement(1791..2618)
                     /gene="sitB"
                     /locus_tag="IU325_21245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HAP5811521.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNASEESIITDDEHPFVAHRPSAVQREAR"
     gene            complement(2618..3508)
                     /gene="sitA"
                     /locus_tag="IU325_21250"
     CDS             complement(2618..3508)
                     /gene="sitA"
                     /locus_tag="IU325_21250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HAP5811522.1"
                     /translation="MLLGCLALTCSIAFQASATEKFKVITTFTIIADMAKNVAGDAAE
                     VSSITKPGAEIHEYQPTPGDIKRAQEAQLILANGMNLELWFQRFYQHLNGVPEVIVSS
                     GVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQRNADTYKA
                     KITQTLAPLRKQIAELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQV
                     RKVVDMVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTENGPVPTYID
                     LLKVTTSTLVQGIKAGKREK"
     gene            complement(4117..4551)
                     /gene="uspF"
                     /locus_tag="IU325_21255"
     CDS             complement(4117..4551)
                     /gene="uspF"
                     /locus_tag="IU325_21255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HAP5811523.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(4692..5825)
                     /gene="ompN"
                     /locus_tag="IU325_21260"
     CDS             complement(4692..5825)
                     /gene="ompN"
                     /locus_tag="IU325_21260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HAP5811524.1"
                     /translation="MKSKVLAFLIPTLLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
BASE COUNT         1599 a         1417 c         1392 g         1455 t
ORIGIN      
        1 gatttgcacc tcctcccaac gtccgcccat gactttctgg actgcagtgt actagcggac
       61 aatgctgccc tcggatgttg tgctaattgg gggataacag atcctgtcta tgagcgtttc
      121 cctttgtgcg tgcgttgaaa ctgataacgg taaacgtcat gatgaacaca accgcgaata
      181 agacgacaat cgtcggagct ggtgcgctgt cgataaaaaa ggacagccaa acaccagtca
      241 ttgagactat taccgatatg ccagtcgcca gtaggagtgt aatatgaaag cgttgtgtca
      301 ggagtaccgc aatggcgcca ggcgcaatga gcagagaaat ggagagaatg atacctactg
      361 ctttcagcgt cgccacaatg gtcagggaaa ccatgcacag cagcccataa tgcagccatc
      421 gcgtatgcaa accgcttacc tgcgcctgtt gataatcaaa gctgaacagc aaaaaatccc
      481 gccattttac gctaataaca agtgtaacca gcccggcaat aatcatcgtc tggattatat
      541 cgccgatggt taccccgagc atgtcgccga aaagaatatg gtcaagatgt atgtctggtt
      601 tgactgcaat atacaagatc agacctgcgg caaacatgcc agaaaaaaca atccccatca
      661 ctgtatcttg cttgattcgg ctgttgtctt tcaggtatcc cgtcgccacc gcgcagaata
      721 ctccggcgat aaatgcccct gtagccagag gtaaccccag aatccaggca agcacgatgc
      781 caggaaaaac cgcatgactc atcgcgtcac ccatcagtgc ccaacctttc aggaccagaa
      841 agaccgacag cagcgcacag ggtatggaaa ctaccagtga aatcagcagt gcgtgactca
      901 taaaggtgaa ctgcagtggc tccagtaata atgccatcat gagcgatcct ccagcgcttt
      961 atgcgcacgc cgacgatttg ccaaaagccc atgggtgggc gcaaatacaa atgctaaaag
     1021 aaacagcagt gtctgcgcca ccacgatgat tccacctgtc gcgccgtcca gaaagtaact
     1081 gacccacgcg ccgaggaaac tggttacact gccaatagtt acagcaatta tgagcaggcg
     1141 gggaaaacga tcggttaaca accatgctgt tgcaccggga gtcaccacca gacagatcac
     1201 cagaaaggca ccaaccgttt gcagcgccgc aacggtcgaa accgacagca gggtgaagaa
     1261 gaggattttt aatctgcccg gatgcaatcc aatagcacgg gcgtgattct cgtcgaaaaa
     1321 ggtcaccata agatctttcc atttgaaaaa aagcacgatg atcgagagaa tgccaatgat
     1381 agtgagttgc aggatatccg ccggatcgat tgccagaata ttgcctagga cgatggtctg
     1441 aatgttcacg gatgtcgggt tcaatgacac cataaacagc cccaggccaa aaaaggagga
     1501 gaagatcagg ccgatgatgg catcttcttt taagcgagtg cgttggttaa gaaacagcat
     1561 gctgcccgct gccagtccgc cggaaaagaa ggctcccaga gaaaacggaa gccccagcat
     1621 atatgccccc gcaacacccg ggacaataga gtgcgacagc gcatcgccaa tcagcgacca
     1681 gccttttagc atcagatagc atgacaggaa agcgcagaga ccgccgacca tcgccgagac
     1741 ccacatcgca ttgagcatat attcatagct gaagggttcc agtagcacgt tcatcttgct
     1801 tccctctgca ccgctgacgg tcgatgtgcc acaaaagggt gttcatcgtc agtaataatg
     1861 ctttcttccg aggcgttaag tgtgacatgg cgcagtacgc cgctaaaagc cagctccagg
     1921 ttctcggctg taaaagtggt gtctgtcggc ccgcttgcca ggacggtgcc tttgaccatg
     1981 accgtataat cgcaaaatgt cgtgactgag ccgagattgt gcgttgagac aagcatcgtc
     2041 ttgccttccg cgcgtaactc cctcaacagg ctgatgattt tcgcttcggt tttcacatca
     2101 acgccggtaa aaggttcatc cagcaggatc acatcaccct gctgtgcaat cgctctcgcc
     2161 agaaagacgc gtttcttttg tccaccggaa agctcgccga tttgtcgatg gcgaaaatcc
     2221 accatatcga cacgttccag cgcatcggtg acaatctgac gatccctctt tttggctata
     2281 cgcaacatac ccatgtgccc atagcggccc atcatcacca catcttccac tagtacagga
     2341 aatgaccagt caacctcttc tgactgaggg acgtaggcaa ccaggttttt ctgtagcgcc
     2401 tgtcgcgtgg gaatacccag aacagatatc tttccgctgg tcagacgcac aaatcccata
     2461 atcgctttaa acaacgtcga ttttccggaa ccgtttaccc caaccagagc ggcaatagat
     2521 ccacttggta ctgtgaagga tgcatcgcgc agtgctgtgt gcccgttacg ccaggtgaca
     2581 gtaacatcat taacgacaat gcccgcagat tgcatcatta tttctccctt ttcccggctt
     2641 taattccctg taccagcgta ctggtagtaa ccttcagaag gtcgatatac gtaggtaccg
     2701 ggccgttttc tgtgctcagg gaatcgacat agagtacacc gccgtagtgc gcgccggttt
     2761 cacgcgcaac ctgacgcgct ggtttatcgg aaatcgtact ctcgctaaag actgccggga
     2821 tatgattttt cttcaccata tcaacaacct tacgtacctg ctgcggcgtg ccttgttgat
     2881 cggcattaat cggccacaga taaagctctt ttagccccaa atcgcgtgcg agataagaaa
     2941 aagccccttc actggtgacc atccatcgct gattctcagg gagttcagca atctgcttac
     3001 gcaggggggc aagggtttgg gtaatcttgg ctttataagt atcggcatta cgttggtagg
     3061 tttgtgcatt tgccggatcg tattttatca acgcatcacg aatattatcg acgtaaatca
     3121 gagcattatc tggcgacatc caggcatggg ggttaggttt gccctcatag gggccttcag
     3181 tgatccctac tggcgtcaca cccgaagaga caattacttc tggaacccca ttaagatgct
     3241 ggtaaaagcg ttggaaccac aattccagat tcataccatt ggcgagaatc agttgtgcct
     3301 cctgcgcacg tttaatatcg ccaggggtag gctgatactc atgaatttct gcaccgggct
     3361 tggttatgga tgagacttct gcagcatctc cagccacgtt tttggccata tctgcgatga
     3421 tggtaaatgt tgtaatgacc ttgaattttt cagttgcact cgcctgaaat gcgatcgagc
     3481 aggtgagggc gaggcacccc aagagcatgg ttactttttt tattgagtac ataatttagt
     3541 acctatagtg agcaaaatta gaacaaacat agcaacggct atgttttata gcataagcaa
     3601 tacaaaatga aaatgattat cattagcaat tttagatccg aaccgggatt aggttgtttc
     3661 ctgctggcga cgtggtactg aggaaaaaag tagggcacct attaggcttt gaaatgactt
     3721 gatgaggaaa attgttgatg gcaaaaatgg ggcaaagcgc tgcaaaaggg gcaaaaaagg
     3781 ggcaaaaaaa gagtgggtta tcgtagctta ttgttgtcgc tgatgatatt taacacattg
     3841 aaaaataagt aaaatactta tgagtcagag agttgtgatt tttgccctta cttgttcagg
     3901 ttgtattgtt ctttcttact aatttcttga ttttgcgaca tttaaaagcg actcaattcg
     3961 ttatatggca tcagaagagt atgcgtcatg ccggaacgcc cagcataaga aatctgatat
     4021 aaaaactgtg gcgtgtatgg tacggattag aggggaaaat gtcagcacat ttgcgaaatg
     4081 aatcaaaaag cccgcagcaa tgtgcgggcg ttagtgtcag cgcacaacca gcacggagca
     4141 ctctgcgtga cgcacgacag ctgcggcgtt ggaaccgagc agataagtgg tgatatccgg
     4201 tcgatgggaa gcaatgatga tcatatgagc ggggatcttc ttcgccaatt ccagaatgcg
     4261 gtctttgggc gagccttcct caacatggac atgcactctg tcggttggca gtttaaattt
     4321 tttaatgatc tcttccagtt gcgatttggc ttccgctttc aggtcatcca ttgccggtaa
     4381 ttctgcggaa tacgctaaac ccagagaggc atagtagggc agtgaaggta ttaccgtcag
     4441 gaaatgaacc tctgcatcat caatctttgc ctctgcctca acgtggctaa tcacgcgttg
     4501 agttaattct gaatcggaaa tatcgatagg gacaagaatc gttctgttca taaaacctcc
     4561 tgttttagta tccgcataaa gtgtaacgcc agatgacact ttttgtgtaa tgacgaagtt
     4621 cacattttta atttagatca aaggaggaag aataagcaga aaaagcccgc cataacagcg
     4681 ggcaggagga tttagaactg ataaaccaga cctaaagcga caatatcatc ggtagagatg
     4741 ccgttggcag cgtagaagct gtcatcttca tccaacaggt tgattttata gtcaacgtag
     4801 gtggacatat ttttgttgaa atagtaagtc gcgccaacat cggcgtattt aaccagatct
     4861 ttatcatcaa cacctgccgg gttgtctgca ccaccggcag cgtgcaggtc acggccttta
     4921 gacatcagga aagagactgc cggacgcaga ccaaaatcaa actggtactg tgcagtaact
     4981 tcaaagttct gggttttgtt agcaacagca taatcgctgt cgccaaacgg ggtcatatta
     5041 cgcgtttctg aatacatggt tgccaggtaa atattgttag catcgtattt tagcccagca
     5101 gtccacgcgt ctgctttatc accacccgcc gcagtatggt taacctggtc attggtgcgg
     5161 tcagaagagg tgtatgccgc accagcgcta aagcccatgc ctaaatcata tgttgtggaa
     5221 agaccccagc cgtcaccgtt ttcatggcga acatcacgtc cgttgttggt gccttcctga
     5281 ccattactgg caccttcgtt gttaccttga tactgcaccg cgaagttcag accatttacc
     5341 agaccgaaga aatcagtatt acgataagtc gcgacgccat tggcacgacc agtcataaag
     5401 ttgtctgcat tggtataaga gtcaccgcca aattcaggca gcatatcggt ccagccttcg
     5461 atgtcgtaca ttacgccata attacgtccg taatcgaaag aaccgtaatc tgcaaatttc
     5521 aatccggcaa atgccagacg ggtccatgac tggttttttg aagattcagt gttgtttgcc
     5581 tgaatattgt attcccattg accgtagcca gtaagttgat cgttaatttg ggtttcgcct
     5641 ttaaaaccca gacgcgcata gctctggtcg ccatctttcg ctgaattatc agaaaaataa
     5701 tgtagtccat caactttgcc atacagatct aatttgttgc cgtctttatt ataaacttcg
     5761 gctgcatgtg cagcacctgc ggcgagcagg gtaggaatta aaaatgccag tactttgctt
     5821 ttcattgaat aaatccttta gttattttat ttgccttttc atc
//
LOCUS       DACBVI010000216         5837 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.218, whole genome shotgun sequence.
ACCESSION   DACBVI010000216 DACBVI010000000
VERSION     DACBVI010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.218"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(117..770)
                     /locus_tag="IU325_21265"
     CDS             complement(117..770)
                     /locus_tag="IU325_21265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAP5811525.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPIQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(763..1647)
                     /gene="fdxH"
                     /locus_tag="IU325_21270"
     CDS             complement(763..1647)
                     /gene="fdxH"
                     /locus_tag="IU325_21270"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAP5811526.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(1660..4707)
                     /gene="fdnG"
                     /locus_tag="IU325_21275"
     CDS             complement(1660..4707)
                     /gene="fdnG"
                     /locus_tag="IU325_21275"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(4120..4122),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAP5811527.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWDEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLQSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            4939..5820
                     /gene="yddG"
                     /locus_tag="IU325_21280"
     CDS             4939..5820
                     /gene="yddG"
                     /locus_tag="IU325_21280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HAP5811528.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGCLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         1319 a         1612 c         1441 g         1465 t
ORIGIN      
        1 aataactggc tcaaagctga aagctttccg gaacccccag cctagctggg ggttttctgt
       61 gcacaaaaaa atggcgcttc ttaaagcgcc attttcagct taaagttcaa aagagattat
      121 atcccttctt cactctcttt tttcgcttct gccttctcga tttcacgata ccagcgcgga
      181 tggtgtttct tcgcccagcg acggctcacc tttccttcga tcatcccttt aatcgatcct
      241 ttcacccaga atgccatata catatggatc aggatggcgt ggatcaggat gatacccgca
      301 gccgcgtgga tcagcaggct ataacggaca acctgtatcg ggaagtactg cgcaaagtac
      361 ggacgccaga taatcacccc ggtcaccagc agcacgaaaa tcatgctcat gatcgaccag
      421 aacatcatct tttgcccggc gttgtactta ccgacatccg ccactttgtg ctcattgcct
      481 tttaatactt cgacaatgtt caacagccac ggaatatctt tcttatccgg gatgttgtga
      541 tgcacaaaac gcacaaacat aaacatcagc gcgacgaaaa tcgcaatgcc gaagaacggg
      601 tgcaaaatgc gtcccatctg cggcgtaccg aaggtttgcg tcagccattg cagcgtcggg
      661 aagaagaacg aaatcccgga cagcgccacc aggaagaagc aaatcaccac ggtccagtga
      721 caggcgcgat caataaattt ggtgcgcaca atcattttcg acttactcat gatgatcctc
      781 ctcgtcatcg tccacttcct tattcgggcc aataccgatg tagtggaaaa tcaacccggc
      841 aaaagtggca ataaagccag ccgctgccag cggtttcagc gcgcctttcc acaggcttac
      901 cgaggtgtcg atcttcggat ctttcggcag accgtgatac agctccggct gatcggcgtg
      961 atgcagcacg tacataacgt gcgtaccacc gaccccttcc gggttgtaga cgccagcatg
     1021 ttcgtaacca cgcgctttca gtttcgccac gcgctgttcc gccagctcca gcatctcctt
     1081 cttggtgccg aagtggatag ccccggtcgg acaggttttc acacaagccg gttcctggcc
     1141 gacgctgacg cgatcgacgc agagcgtgca tttatatacc cggttatcct ctttgttgag
     1201 gcgcggaata ttaaacggac acccggcaat gcagtaacca cagccgatgc agttttccga
     1261 ctggaaatcg acaatcccgt tagcgtactg aatgattgca ccagcagacg ggcacgcctt
     1321 caggcagccc ggatcttcac agtgcataca gccgtcttta cggatcagcc actccagctt
     1381 gccgttctgt tcggtttcgc taaagcgcat caccgtccag gacttggcgc tcagatcggc
     1441 ggggttatcg taaaccccga cgcagtgccc cacttcatca cggatgtcgt tccactccga
     1501 acacgccacc tgacaggctt tacaaccgat acaggtggaa acgtcgataa gtttcgcgac
     1561 ttctgctttg taatcacgca cctgagaagg cggcgtgatg gagttagttg cggacctttt
     1621 gataatgtcc tgcgtttcca tagccatctg ttcgccccct tacgcctttt cgatgttgac
     1681 taagaacgct ttatattccg gcgtttgcga gtttgcatca ccgacattcg gcgtcagagt
     1741 gttagcgata taacctttac gcgcgacacc ctcaaagccc cagtggattg gaatacccac
     1801 cgtttcaacc tgctgaccgt ttacattcag cggtttcaga cggcgcgtta ccaccgccac
     1861 cgcgcggata aatccacgct tgcttgagac agtgacacga tcgccattat taatgccttt
     1921 cgccgccgcc agcgtttcgc tgatttccac aaactgttcc ggctgagcaa ttgcgttgag
     1981 caacgcgtgc ttggtccagg tgtggaagtg ctcggtcaga cgataggtcg tacccacata
     2041 cgggaactgc tcttttttac ccatccgcag cgcgtcttgt tcatacagac gaacaaccgg
     2101 gttagacacc acattcggat gcagcgggtt agtgcccagc ggcgtttcga ttggctcgta
     2161 gtgttccggg aacggacctt ccgccatttt gttgatggca aacaggcgtc ccatcccttc
     2221 cggctgcatg ataaacggtc cggttggtgt acctggcgcg gcattgccga agtccggaat
     2281 atcgttaccc gtccacttgc tgccgttcca ctggatcagc atccgtttcg gatcccacgg
     2341 tttaccgttg atatccgccg aagcacggtt gtagagtacg cgacggttga gcggccacgc
     2401 ccaggcccat cccagcgtat tccccagacc ggacgggtcg gagttatcgc ggttagccat
     2461 ctggttgccc tgctctgtcc agctaccagt gtagatccag caagaagatg cggttgtacc
     2521 gtcatcacgc agatgcgcaa agctactcag caactgccct ttcttcgcaa tcagcacacc
     2581 attagcatca tagagatctt ccagcgcata gccgttgttc tctttagcta cttcgtcaga
     2641 ttgcggttcg tgcggctgct tgtagttcca gctcatcttc atcagcggtt ctacgccttt
     2701 accaccttcg gcctggtaca gctcgcgcag atgatggtag atacccgcca gaatttcgcc
     2761 gtcgttacgc gcttcgcccg gcgcgtcctg acctttccag tgccactgca accagcgacc
     2821 ggagttagca atagaaccat cttcctcagc aaagcaggtc gaaggcagac ggaatacttc
     2881 agtctgaata gacgcaggat cgacatcgtt cgactcgccg tggttctgcc agaaggtaga
     2941 agtttcagtc accagcggat cgataaccac catatacttc agcttgctca ggcagcttac
     3001 cactttgttt ttgtccggga aggacgcaac cgggttaaag ccctggcaga aataaccggt
     3061 gactttgcct tcatccatca tgttgaaata cttgatgacg tcgtaggtct ggtcccactt
     3121 gggcagccag tcgtagcccc agttgttctc tttctgcgca gcatcgccgt agaaagattt
     3181 catcaggcta acgaagaact tcggatagtt gctccagtag ttcacctgat cagccagcgt
     3241 cgctttcggc gtgttcgctt ccagatacga ctgcaaatca acctgttttt ctgactgcag
     3301 cgtcagataa cccggcaggc tggtagagag caggcctaag tcggtcagcc cctgaatgtt
     3361 ggagtgacca cgcaatgcgt tcacgccgcc accggccata cccatgttac cgagcagtaa
     3421 ctggatcatc gccatagtac ggatgttctg cgcacccacg gtgtgctgcg tccagcccag
     3481 cgcgtaaagg aaggtggttg tgcgatccgg tgcgctggtg gaggccagca cttcacacac
     3541 tttcaggaag tcggcttttg gcgtaccaca gatgttttct actacgtccg gcgtgtagcg
     3601 ggaaacgtgc tctttcagca agttccacac acagcgcgga tgagtcagtg tttcatcgcg
     3661 tttcgcatag ccgttttcat cgaactgata gttccaggac gatttatcgt attgacgttt
     3721 ttcagcgtcg tagccgctga acagaccgtc ttcgaaagca aaatcatcac gcaccagcag
     3781 actggcgttg gtgtaatgct taacgtattc ggcgttgatt ttgttgtttt cgatcaggta
     3841 gcgcaaaacg ccagacagga acgtaatgtc cgtaccggaa cgaataggtg cgtaaatatc
     3901 cgctacagaa gcggtacgcg taaaacgggg atcgacaacg atcaaggttg catcgttgtt
     3961 gtttttcgct tccatcgccc agcggaaacc gacgggatgc gcttcagcag cgttaccgcc
     4021 catcaccatt acgacgttag cgtttttgat atccacccag tggttggtca tcgcaccgcg
     4081 accaaatgtt ggagcaagac ttgctaccgt tggtccgtgt cagacgcgcg cctggttgtc
     4141 taccgccagc atcccgaggg agcgggcaaa tttctgggtc agcatcccgg tttcgttgct
     4201 ggcaccggag gcacacagca taccggtaga aagccaacgg tttaccgtta cgccctgctc
     4261 gttcttttca ataaagttag cgtcacggtc agctttcatc agcttcgcaa tacgggagaa
     4321 tgcttcatcc cagctaatgc gctgccattt gtcagaacct ggcgcacgat attccgggta
     4381 gcgcagacgg ttttcgctgt tgacgtaatc cagcaaaccg gcccctttcg ggcataacgc
     4441 accacggctt accggatgat ccgggtcacc ttcaatgtga taaatcgctt ctctggcgtt
     4501 tttcgcgcca tcacccaggc tatacatcaa tagcccgcaa cctacggaac agtatgtgca
     4561 ggtgttacgg atctctttag cgcgtaataa tttatagttt cgcgcctgag ccagtgcttg
     4621 cttcggggca aagcccaatg ctgctactgt tgttcccgcc ataccgcccg cgcagatttt
     4681 aaaaaattgt ctgcgactga cgtccattgc tttcctcttt tttcaggggt attactgcgc
     4741 gaggaaacta acagtaaaat tctgaatctt tttgcgcgaa atcaaaaaga acacgtgttt
     4801 gccacaaaat aatccgcctg cgcaaattga cctacctcaa tagcggtaga aaaacgcacc
     4861 actgcctgac aggccagtta aaaaaatgct ataaaattca gcttaatttt taacggcaag
     4921 agagacaaaa cagcgagcat gacacgacaa aaagcaacgc tcatagggct aatagcgatc
     4981 gtcctgtgga gcacgatggt aggattgatt cgcggtgtca gtgaggggct cggcccggtc
     5041 ggcggcgcag ctgctatcta ttcattaagc gggctgctgt taatcttcac ggttggattt
     5101 ccgcgtattc ggcagatccc gaaaggctat ttactcgccg ggtgtctgtt attcgtcagc
     5161 tatgaaatct gtctggcgct ttccttagga tatgcggcga cccgtcatca ggcgattgaa
     5221 gtgggtatgg tgaactatct gtggcccagc ctgacaattc tctttgccat tctgtttaat
     5281 ggtcagaaaa ccaactggct gattgtacca ggattattat tagccctggt tggcgtctgc
     5341 tgggtgttag gtggtgacaa tgggttacat tatgatgaaa tcatcaataa tatcaccacc
     5401 agcccattga gttatttcct ggcgtttatt ggtgcgttta tctgggcagc ctactgcaca
     5461 gtaacgaata aatacgcacg cggatttaac ggaattaccg tttttgtcct gctaacggga
     5521 gcaagtctgt gggtttacta ttttctcacg ccacaaccag aaatggtatt tagcacgcca
     5581 gtcatgatta aactcatctc tgcggcattt accttaggat ttgcttatgc tgcatggaac
     5641 gtcggtatat tacatggcaa cgtcaccatt atggcggtag gttcgtattt tacgcccgtc
     5701 ctttcctcag cgcttgcagc cgtgctgctc agcgccccgc tgtcgttctc gttctggcaa
     5761 ggcgcgctaa tggtctgcgg cggttccctg ctctgctggc tggcgacacg tcgtggctaa
     5821 aataattgtc gggtcta
//
LOCUS       DACBVI010000217         5757 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.219, whole genome shotgun sequence.
ACCESSION   DACBVI010000217 DACBVI010000000
VERSION     DACBVI010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.219"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            44..1099
                     /gene="phoE"
                     /locus_tag="IU325_21285"
     CDS             44..1099
                     /gene="phoE"
                     /locus_tag="IU325_21285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAP5811529.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1138..1539)
                     /locus_tag="IU325_21290"
     CDS             complement(1138..1539)
                     /locus_tag="IU325_21290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAP5811530.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(1597..2841)
                     /gene="frsA"
                     /locus_tag="IU325_21295"
     CDS             complement(1597..2841)
                     /gene="frsA"
                     /locus_tag="IU325_21295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAP5811531.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(2933..3391)
                     /locus_tag="IU325_21300"
     CDS             complement(2933..3391)
                     /locus_tag="IU325_21300"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP5811532.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3652..5109
                     /gene="pepD"
                     /locus_tag="IU325_21305"
     CDS             3652..5109
                     /gene="pepD"
                     /locus_tag="IU325_21305"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAP5811533.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5166..5497)
                     /locus_tag="IU325_21310"
                     /pseudo
     CDS             complement(5166..5497)
                     /locus_tag="IU325_21310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5466..5720)
                     /locus_tag="IU325_21315"
     CDS             complement(5466..5720)
                     /locus_tag="IU325_21315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811534.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAALFSVGRFYPARRRGNGAGNRNGPLL"
BASE COUNT         1479 a         1451 c         1428 g         1399 t
ORIGIN      
        1 ctcccgctga atattcgcgc gttaattaaa atcaggaatg aaaatgaaaa agagcactct
       61 ggcattagtg gtgatgggca ttgtggcatc tgcatccgta caggccgcag aaatatataa
      121 caaagacggt aataaactgg atgtctatgg caaagttaaa gccatgcatt atatgagtga
      181 taacgacagt aaagatggcg accagagtta tatccgtttt ggttttaaag gcgaaacaca
      241 aattaacgat caactgacgg gttatggtcg ttgggaagca gagtttgccg gtaataaagc
      301 ggagagtgat actgcacagc aaaaaacgcg tctcgctttt gccgggttga aatataaaga
      361 tttgggttct ttcgattatg gtcgtaacct gggcgcgctg tatgacgtgg aagcctggac
      421 cgatatgttc ccggaatttg gtggcgactc ctcggcgcag accgacaact ttatgaccaa
      481 acgcgccagc ggtctggcga cgtatcggaa caccgacttc ttcggcgtta tcgatggcct
      541 gaacttaacc ctgcaatatc aagggaaaaa cgaaaaccgc gacgttaaaa agcaaaacgg
      601 cgatggcttc ggtacgtcat tgacatatga ctttggtggc agcgatttcg ccatcagtgg
      661 ggcctatacc aactccgatc gcactaacga gcagaacctg caaagccgtg gcacaggcaa
      721 gcgtgcagaa gcatgggcaa caggtctgaa atacgatgcc aataatattt atctggcaac
      781 tttctattct gaaacacgca aaatgacgcc aataactggc ggctttgcca ataagacaca
      841 gaactttgaa gcggtcgccc aataccagtt tgactttggt ctgcgtccgt cgctgggtta
      901 tgtcttatcg aaagggaaag atattgaagg catcggtgat gaagatctgg tcaattatat
      961 cgacgtcggt gctacatatt atttcaacaa aaatatgtca gcgtttgttg attataaaat
     1021 caaccaactg gatagcgata ataagctgaa catcaataat gatgatattg tcgcggttgg
     1081 catgacctat cagttttaat ggatattgcc ggatgtgatg catccggcac atttcactca
     1141 cgccgttaac ttcaccggtt catcacgaaa atcatccgcc ggttccagct tcagattcag
     1201 cgtcgccagc agctcacgca gcttctcgtg aaattcacgc agggtgtgct ccagtcgttc
     1261 aaccacttcg gcatctttta tctgaacact cgtccagtcg cctgctttat cgaacagacc
     1321 aaactggtaa ctgtaggtaa aacgggattc ctgcgcttcc agttccatcc accagcccca
     1381 gaactcacgc acttccggtg ccggtttcac gttgacgcat acagccagac aatcgaaaaa
     1441 gaatcgatta tcttcgcact taccttcacg aatatacggg cctagtgcgg taaatttttt
     1501 gatcaatctg ctcttcgggt gtccactcgg taacgtcatt gcaatctcct gttgtgatgc
     1561 aactgtttta ccaaattggc aaaatttagc aaatttttaa cacaatcgtt tttcaatcca
     1621 gtctgttatt tcctgaagac ctttgtcaaa attccgatac accgggttaa atgggatctc
     1681 taacaattta ccgtcagcag atgatgaggt gattaagcgt gagtcctctt ccgggctgaa
     1741 cggatcgttc ttccagtagc ctgataacat tggcgttggg cagcgacgtc ccagcaatcc
     1801 ttgcactttt aatgaatagc gattcagctc cacgcgcaac gcttcgtcgg aagcatcatg
     1861 catccccaaa cggctcgcca gaacgtcaag atacatttcc ggcacctgtt gctggcactt
     1921 aaaatcactc aacagggtat gaactaccgg accaagacag gcaaccgctt tcagacgcgg
     1981 cgattcaagg tatgccagac gcacggcaac gttagcgccg aaacggaaac caaaggccgc
     2041 gacgcgagtg tgatctaccc acggtacgtt aggcagcgcc tttaagacgt gctgatgcaa
     2101 caggctggag tcctgggtga gcttccattt tgaagaaaag cccaccgacg gcatatcaat
     2161 agtcagcatc gcaatgccgc gcggcgcaaa ataacgttca tacaggctgt aatagtccgt
     2221 ctgcatcgca tccagaccac cacacattaa tactgtcggg aacgggccat cgcctttcgg
     2281 catatgcaaa aagccggtga tgggcgcacc gccgggtacg gtaaactcca tctgccgcat
     2341 ggtgcccggc agacgctgag cggcctcttc ataggcgcgg tttgacaaag cctgcgcttg
     2401 ctcggccagg tcatctcctt tcagatgagg ataggcggca atgttgtaca acgtagccgc
     2461 atgcagccag tgacgaccac tgagttgcgg atcttcttcc gcacaggctt tttgttgcca
     2521 caccatcgcc tgggtggccc actcataaat ccagttgcca ccacggtagc caatcaccgt
     2581 atcgtataaa tcatcgtcgg tacgttcggc atcgctcatc acaatacgtg cctggacgtc
     2641 gaggatttca cgtgggtcaa tgccgcgcca gatccacatc agacggttaa tcatgcgata
     2701 ccagtgaggg atggttttac catcaagggc cgactgcaca ggcggttgtg cgccgtgatt
     2761 aaagcggcgg actagcgtcg aggtttcagg atgtttaaag cggggtttga acagggtttc
     2821 gctcaggttt gcctgtgtca tggatgcagc ctccagaata cttactggaa actattgtaa
     2881 cccgcctgaa gttaaaaaga acaacgcccg gcagtgccag gcgttgaaaa gattagcgac
     2941 cggagattgg cgggacgaat acgacgccca tatcccacgg ttgttcgatc caggtatctt
     3001 gcgggatatc aacaacatag tcatcaacca gcggacgacc agccggtttt gcgaagatgg
     3061 tgacaaagtg cgcttttgga tacatttcac gaatcgcaac cgcagtgcca ccggtatcca
     3121 ccaggtcatc aataacgatg aagccttcgc catcgccttc tgcgcgtttc agcactttaa
     3181 gctcgcgctg gttgtcgtga tcgtagctgg aaatacaaac ggtatcgaca tgacgaatac
     3241 ccagttcacg cgccagtaac gcacccggta ccagaccgcc acggcttacg gcaataatgc
     3301 ctttccattg ttcagaaggc atcagtcggc ttgcgagttt acgtgcatgg atctgcaaca
     3361 tgtcccaggt gacgatgtat ttttcgctca tgtgaagtgt cccagcctgt ttatctacgg
     3421 cttaaaaagt gttcgagggg aaaataggtt gcgcgagatt atagagatct ggcgcactaa
     3481 aaaccagtat ttcacatgag tccgcgtctt tttacgcact gcctctccct gacgcgggat
     3541 aaagtggtat tctcaaacat atctcgcaag cctgtcttgt gttgacaaca ttttctgcta
     3601 accctgtgac ctgcaatact gttttgcggg tgatcgacaa ggagacttaa cgtgtctgaa
     3661 ctgtctcaat tatctccaca gccgctgtgg gatatttttg ccaaaatctg ttctattcct
     3721 cacccgtcct atcatgaaga gcaactcgct gaatacattg ttggttgggc aaaagagaaa
     3781 ggtttccatg tcgaacgcga tcaggtaggt aatatcctga ttcgtaaacc tgctaccgca
     3841 ggtatggaaa atcgtaaacc ggtcgtcttg caggcccacc tcgatatggt gccacagaaa
     3901 aataacgaca ccgtgcatga cttcactaaa gatcctatcc agccttatat tgatggcgaa
     3961 tgggttaaag cgcgcggcac cacgctgggt gcggataacg gcattggtat ggcgtctgcg
     4021 ctggcggttc tggctgacga aaacgtggtt cacggcccgc tggaagtgct gctgaccatg
     4081 accgaagaag ccggtatgga tggtgctttt ggcttacaga gcaactggtt gcaggctgat
     4141 attctgatta acaccgactc cgaagaggaa ggtgaaatct acatgggttg cgcggggggt
     4201 atcgacttca cctccaacct gcatttagat cgtgaagcag ttccagctgg ttttgaaacc
     4261 ttcaagttaa ccttaaaagg tctaaaaggc ggtcactccg gcggtgaaat ccacgttggc
     4321 ctgggtaatg ccaacaaact gctggtgcgc ttcctggcgg gtcatgcaga agagctggac
     4381 ctgcgcctta tcgatttcaa cggtggcaca ctgcgtaacg ccatcccgcg tgaagccttt
     4441 gcgaccattg ctgtcgcagc tgataaagtc gacgccctga aatctctggt gaatacctat
     4501 caggaaatcc tgaaaaacga gctggcagag aaagagaaaa atctggcatt gctgctggac
     4561 tctgttgcga acgataaagc tgccctgatt gcgaaatctc gcgatacctt tattcgtctg
     4621 ctgaacgcca ccccgaacgg tgtgatccgc aactccgacg tggcaaaagg tgtggtcgaa
     4681 acctccctga acgtcggtgt ggtgaccatg actgacaata acgtagaaat tcactgcctg
     4741 atccgttctc tgatcgacag cggtaaagac tacgtggtga gcatgctgga ttcgctgggt
     4801 aaactggctg gcgcgaaaac cgaagcgaaa ggcgcatatc ctggctggca gccggacgct
     4861 aattctccgg tgatgcatct ggtacgtgaa acctatcagc gactgttcaa caagacgccg
     4921 aacatccaga ttattcacgc gggcctggaa tgtggtctgt ttaaaaaacc gtatccggaa
     4981 atggacatgg tttctatcgg gccaacaatc accggtccac actctccgga tgagcaagtt
     5041 cacatcgaaa gcgtaggtca ttactggaca ctgctgactg aactgctgaa agaaattccg
     5101 gcgaagtaat tatttgattt gctgccggat ggcgtttaat cgccttccgg cagtttcatc
     5161 cttcattatc cttcgataaa agccatccct gtaaatgtcc gtctcgggtt gccacgttca
     5221 atctggtgat ggaacattcg ccgctgcgat ttcagcaccg cgctattttc ctgttgctgt
     5281 tgttccagct tccaggcaat cagcaatcgc gccagccgct tgttagcatg cccagaaaca
     5341 agtttttgcg cccatgatga ggccgatacg gactcggaca aatccactga atagtgccgc
     5401 accaactttc gcttaatgcc catgcgttat cgccatccag agaaatcagc gccgaacgca
     5461 gcgtgtcaga gcagcggccc gtttctgttt ccagcaccgt taccgcgacg tcttgccggg
     5521 taaaaacgac caacagagaa taacgctgcg ccaatcgggt agccgcgtcc ggggtgtaaa
     5581 tctggcatcc ctactacgcg ctgcatgttg ggtaaatttg ccgtggtgtg taattgttgg
     5641 atcgctaaac tctcgatcca caggtcatca gatgcgatgg taaataccgc atcggataag
     5701 ggacgaatat atttgcccat gtatcattcc atttatactc gtcatacttc aagttgc
//
LOCUS       DACBVI010000218         5685 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.220, whole genome shotgun sequence.
ACCESSION   DACBVI010000218 DACBVI010000000
VERSION     DACBVI010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.220"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            371..4621
                     /gene="fdeC"
                     /locus_tag="IU325_21320"
     CDS             371..4621
                     /gene="fdeC"
                     /locus_tag="IU325_21320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
                     /protein_id="HAP5811535.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTGDNNVEKNVASFAANAGTFLSSQPDSDATRNFITGIATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAITAEVNYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKEQIKTEL
                     TFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     LVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVLRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTLTAIWSTKDANDNPVTSLTPEAPSLAGAAAVGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKTPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVATLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASRAVISDMAVKVNNQLANGQSANQVTLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKADIELISTVAGELEIAAAVKNSQKTVTVKF
                     NADASTGQANLQVDTAVQKVANGKDAFTLTATVEDKNGNPVPGTLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNDQPSDAQTITFVADKATATVSGIE
                     VIGNYALADGKAKQTYKVTVTDANNNLLKDSEVTLTASPANLALDPDGTATTNEQGQA
                     IFTATTSVAATYTLTAKVEQADGQESTKTAESKFVADDKNAELAASPERVDSLVADGK
                     TTATLTVTLMSGVNPVGGTMWVDIEAPEGVTEADYQFLPSKNDHFASGKITRTFSTNK
                     PGTYTFTFNSLTYGGYEMKPVTVTINAVPADTEGAEEK"
     gene            complement(4746..5603)
                     /locus_tag="IU325_21325"
     CDS             complement(4746..5603)
                     /locus_tag="IU325_21325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP5811536.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMSKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
BASE COUNT         1523 a         1300 c         1514 g         1348 t
ORIGIN      
        1 tttatcctgg cgtatatacc tacttcgacg tatttccaat agttgttaat taaaggcagg
       61 ccattattat tcactctgaa taatgaatta ttcactgtcc gcagagtaaa aatataactt
      121 aggtatctat ttaataactt gcacaaaaag ctaaattttt acccataaat aaaaatataa
      181 tcccgcgccc aaccacctga tgagtggcta cagacactgg atatattagg tggcggtgta
      241 ctttcttaca taaaggtatt tccttttctg cggaaaagga aatcggggat ttcccggttt
      301 ttctgacaag cagacgccac tatttgtgtc tgcctatgtt cgttaattcg ttcatcagga
      361 aattatctca atgtcacgtt ataaaacagg tcataaacaa ccacgatttc gttattcagt
      421 tctggcccgc tgcgtggcgt gggcaaatat ctctgttcag gttctttttc cactcgctgt
      481 cacctttacc ccagtaatgg cggcacgtgc gcagcatgcg gttcagccac ggttgagcat
      541 gggaaatact acggtaactg gtgataataa cgtggagaaa aatgtcgcgt cgtttgccgc
      601 aaatgccggg acatttttaa gcagtcagcc agatagcgat gcgacacgta attttattac
      661 cggaatagcc accgctaaag ctaaccagga aatacaggag tggctcggaa aatatggtac
      721 agcgcgcgtc aaactgaatg tcgataaaga tttctcgctg aaggattctt cgctggaaat
      781 gctttatcct atttatgata cgccgacaaa tatgttgttc actcaggggg caatacatcg
      841 taccgacgat cgtactcagt caaatattgg ttttggctgg cgtcattttt caggaaatga
      901 ctggatggcg ggggtgaata cttttatcga tcatgattta tcccgtagtc atacccgcat
      961 tggtgttggt gcggaatact ggcgtgatta tttgaaactg agcgccaatg gttatatccg
     1021 ggcttctggc tggaaaaaat caccggatgt tgaggattat caggaacgcc cggcgaatgg
     1081 ctgggatatt cgtgctgagg gctatttacc tgcctggccg cagcttggcg caagcctgat
     1141 gtatgaacag tattatggcg atgaagtcgg gctgtttggt aaagataaac gccagaaaga
     1201 tccacatgcg attaccgctg aagtgaatta cacgccagtg cctcttctga ccctgagtgc
     1261 cgggcataag cagggcaaga gtggtgagaa tgacactcgc tttggcctgg aagttaatta
     1321 tcggattggc gaacctctgg aaaaacaact cgatacagac agcattcgcg agcgtcgaat
     1381 gctggcaggc agccgctatg acctggttga gcgtaataac aacatcgttc ttgagtaccg
     1441 caaatctgaa gtgattcgta ttgctctgcc tgagcgtatt gaaggcaagg gcggtcagac
     1501 actttccctg gggcttgtgg tcagcaaagc aactcacgga ctgaaaaatg tgcaatggga
     1561 agcgccgtca ttactggctg aaggtggcaa aattaccggt cagggtagtc agtggcaagt
     1621 aacgctcccg gcttaccgtc caggcaaaga caattattat gcgatttcag cagttgccta
     1681 cgataacaaa ggtaatgcct cgaaacgtgt gcagacagaa gtggttatta ccggagctgg
     1741 tatgagcgcc gatcgcacgg cgttaacgct tgacggtcag agccgtattc aaatgcttgc
     1801 taacggtaat gagcaaaagc cgctggtgct gtctctgcgc gacgccgagg gccagccagt
     1861 cacgggcatg aaggagcaga tcaagactga actaaccttc aaaccggctg gaaatattgt
     1921 gactcgttcc ctgaaggcca ctaaatcaca ggcaaagcca acactgggtg agttcaccga
     1981 aactgaagca ggggtgtatc agtctgtctt tacgaccgga acgcagtcag gtgaggcaac
     2041 gattactgtt agcgttgatg gcatgagcaa aaccgtcact gcagaactgc gggccacgat
     2101 gatggatgtg gcaaactcca ccctgagcgc taacgagccg tcaggtgacg tggttgctga
     2161 tggtcagcaa gcctacacgc tgacgctgac tgcggtggac tccgagggta atccggtgac
     2221 gggagaagcc agccgcctgc gacttgttcc gcaagacact aatggtgtta ccgttggtgc
     2281 catttcggaa ataaaaccag gggtttacag cgccacggtt tcttcgaccc gtgccggaaa
     2341 cgttgttttg cgcgccttca gcgagcagta tcagctgggc acattacaac aaacgctgaa
     2401 gtttgttgcc gggccgcttg atgcagcaca ttcgtccatc accctgaatc ctgataaacc
     2461 ggtggtcggc ggtacgctta cggcaatctg gtcgacaaaa gatgccaatg acaaccctgt
     2521 gaccagcctc acgccggaag cgccgtcatt agcgggtgcc gctgctgttg gttctacggc
     2581 atctggctgg acaaataatg gtgatgggac gtggactgcg cagattactc tcggctctac
     2641 ggcgggtgaa ttagaagtta tgccgaagct aaatggacag gatgcggcag caaatgcggc
     2701 aaaagtaacc gtggtggctg atgcgttatc ttcaaaccag tcgaaagtct ctgtcgcgga
     2761 agatcacgta aaagccggcg aaagcacaac cgtgacgttg gtggcgaaag atgcacatgg
     2821 caacgctatc agtggtcttt cgttgtcggc aagtttgacg gggaccgcct ctgaaggggc
     2881 gaccgtttcc agttggaccg aaaaaggtga cggttcctat gttgctacgt taactacagg
     2941 cggaaagacg ggcgagcttc gtgtcatgcc gctcttcaac ggccagccag cagccaccga
     3001 agccgcgcag ctgactgtta ttgccggaga gatgtcatca gcgaattcta cgcttgttgc
     3061 ggacaataaa actccaacgg ttaaaacgac gacggaactc accttcaccg tgaaggatgc
     3121 gtacgggaat ccggtcaccg ggctgaagcc agatgcacca gtgtttagcg gtgccgccag
     3181 cacggggagt gagcgtcctt cagcaggaaa ctggacagag aaaggtaatg gggtctacgt
     3241 ggcgacctta acgctgggat ctgccgcggg tcagttgtct gtgatgccgc gagtgaacgg
     3301 ccaaaatgcc gttgctcagc cactggtgct gaatgttgca ggcgacgctt cccgggcagt
     3361 gatcagtgat atggcggtga aggtgaataa ccaactggca aatggacagt ctgctaacca
     3421 ggtaaccctg accgtcgtgg acagctatgg taacccgttg caggggcaag aagttacgct
     3481 gactttaccg cagggtgtga ctagcaagac ggggaataca gtaacaacca atgcggcagg
     3541 taaagcggat attgagttga tatcaaccgt tgcaggggaa cttgaaatcg cggccgcggt
     3601 gaaaaactct cagaagacgg tcacggtgaa attcaacgcg gatgccagca ccggtcaggc
     3661 aaacctgcag gtagacaccg ccgttcaaaa agtggcaaac ggcaaagatg cctttacgct
     3721 gacggcgacc gttgaggata aaaacggtaa ccctgttcca ggcactctgg tgacctttaa
     3781 tctgccccgg ggtgtcaagc cgcttactgg cgataatgtc tgggtgaaag ccaacgatga
     3841 ggggaaagca gagttgcagg tggtttccgt gactgccgga acctatgaga tcacggcatc
     3901 ggcaggaaat gaccagcctt cggatgcgca gactataacg tttgtggccg ataaggcgac
     3961 cgcaaccgtc tccggtattg aggtgattgg caactatgca ctggcggatg gcaaagccaa
     4021 acagacgtat aaagttacgg tgactgatgc caataacaac ctgttgaaag atagcgaggt
     4081 gacgctgact gctagcccgg caaatttagc tctggatccc gatgggacgg cgacaacgaa
     4141 tgagcaagga caggctattt tcaccgccac gaccagtgtc gcagcgacat atacactcac
     4201 ggcgaaagtg gaacaggccg acggtcagga atcgacgaaa actgccgaat ctaaattcgt
     4261 cgcggatgat aaaaacgcgg agctcgctgc atctccagag cgtgtagatt ctctggtggc
     4321 ggacgggaag actactgcaa cactgacggt tactctgatg tcgggtgtca accccgtagg
     4381 aggaaccatg tgggtcgaca ttgaggctcc ggaaggggtg acagaggcgg attatcagtt
     4441 cctgccgtcg aaaaatgacc atttcgcgag cgggaaaatc acgcgtacat ttagtaccaa
     4501 caagccaggt acatacacat tcacattcaa ctctttgaca tatggagggt atgaaatgaa
     4561 accagtgact gtgacaatta acgccgttcc tgcagatact gaaggtgctg aggagaaata
     4621 aaaacgacga tgtaataaat aataatggcg ttgacatgcg cagtgccatt atttactgaa
     4681 tacctaccct ggtaccgatt agacggtggc agggtagggt aaatcttatg tgatggattt
     4741 acaggctaaa ccgggcgaac ggggataaaa atttcgcaaa caatgttatt gctatcaatt
     4801 tcgacgaagt tttcattgta ataaaatcgc tcaatatcac tgccgttcac tctggctaac
     4861 cccctggcag gtaattccat cagatagagg tttcgcgccc attcagggta gtgctcccac
     4921 tcaccttcat agcggaaaga ggcatacagt cctcggttaa ttgtccactc tttcgacatc
     4981 tctgaatcgg tttgtttgca taccgtaccg gcaaaagttt caactgtgag caaatcgacc
     5041 gattttgttg acggttcgaa tgtggagacg aaatagatat ccgtcttaaa attcttcata
     5101 catctggcta aatgatgacg tcgcagtttg acttcttcat ccggtgattt attcgatact
     5161 tcaatatcat atttaaatga attgccaaaa ataggtgtct caggaaagtt gactaatcta
     5221 cactccgtct tttgctgttg acgtattaaa aaagaaggga agatatttgc gagatcccaa
     5281 taatcacggt ggcggtattc acggggagag cagccgaata actttttaaa ttcgcggctg
     5341 aatgactgct gtgagtcaaa atgtaaagag agtgcaatat caagcataga tttcgcggtg
     5401 agtcggacaa gaatggcggc acgacaaagc cttcgtttgc gaatgtattc tcccaaaggc
     5461 acatgcatga aatttcggaa cagaagctgg atgttgcgtc tgctgtagcc agatttctgt
     5521 gcgatatctt cgattgaaat agggtgctca agattgcact caatccactc caggagctgc
     5581 tgtagaatct tctgcctgat catcacattc tccgttaatt ctttgtaata tcgcacattt
     5641 tcgttatgtc ctgggcctta agtgtatcaa aaacagcaag tccca
//
LOCUS       DACBVI010000219         5668 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.221, whole genome shotgun sequence.
ACCESSION   DACBVI010000219 DACBVI010000000
VERSION     DACBVI010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.221"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(43..1596)
                     /gene="caiC"
                     /locus_tag="IU325_21330"
     CDS             complement(43..1596)
                     /gene="caiC"
                     /locus_tag="IU325_21330"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HAP5811537.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSSGVVNRYSYLELN
                     QEINRTANLFYALGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDASQLRHICLIDMALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHCLREVMFYLNLSEQEKDAFCERFSVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGELGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            complement(1670..2887)
                     /gene="caiB"
                     /locus_tag="IU325_21335"
     CDS             complement(1670..2887)
                     /gene="caiB"
                     /locus_tag="IU325_21335"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HAP5811538.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMSQYFMMDYFNG
                     GEMCPRMTKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTSEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGKIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            complement(3016..4158)
                     /gene="caiA"
                     /locus_tag="IU325_21340"
     CDS             complement(3016..4158)
                     /gene="caiA"
                     /locus_tag="IU325_21340"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HAP5811539.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            complement(4189..>5668)
                     /gene="caiT"
                     /locus_tag="IU325_21345"
     CDS             complement(4189..>5668)
                     /gene="caiT"
                     /locus_tag="IU325_21345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HAP5811540.1"
                     /translation="KVFFPPLIIVGILCWLTVRDLDAANVVINAVFSYVTNVWGWAFE
                     WYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSAAVLFWGSIEIYY
                     YISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYFFFVRKMEVIRPS
                     STLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTECMQWLFGIPHTLQ
                     LDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIVSGASFIMNYFTD
                     SVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIFLARISRGRTVRE
                     LCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVARAIIETWAALPLS
                     TATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRIGWSILVGIIGIV
                     LLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
BASE COUNT         1369 a         1527 c         1437 g         1335 t
ORIGIN      
        1 agcgtgatta ttagctggca gggtagttcc ctgccgtttc atttatttaa gattctttct
       61 aattattttc cccgagcaat tacgtggcag atcttttctg atctccagat aagagggcac
      121 tttaaatttc gccatatttt gttcgcagaa gcggaaaaat tcctcttcgc tcaatgtttc
      181 accttcattc agcaccacaa atgctttgat ggcttcatcg cgtatcgaat ctttaatacc
      241 cacaaccacg atatcctgaa ttttgggatg ggtggcgatg atattttcca gctccacgca
      301 ggagacattc tcgccaccgc gtttgatcat attgcagcgg cgatcgacga aataaaaaaa
      361 gccctcttcg tcgcggtatc cggtatcgcc ggtatgtagc cagccatcgg cttccagtac
      421 tttcgcagtg gctttcgggt tgagaaaata ctctttgaag atggttttcc ctggtacgcc
      481 tttaatacag atttcaccga gctcaccagc cgggagggga cgattgtgat cgtcgcggat
      541 ctccgcttcg tagcaaaagc ccgcccgacc aatcgacggc cagcgtcgtt tatcgccagg
      601 gcgatcgccg ataatgccca caatggtttc cgtcattcca taagacgtca gcaagcgaac
      661 gctgaagcgt tcacaaaacg cgtctttttc ctgctccgac aggttgagat aaaacatcac
      721 ttcccgcagg cagtgttgcc gatcgttcgc tgaaggcggc tgcaccatca acgtacgaat
      781 catcatcgga atacattcgg taatggtggc gcggtacttc tgcacctgtc cccagaaggc
      841 gcgggcgctg tatttctcga caagcacaaa ggtggccccg gcagaaaacg ccgccatcgc
      901 cgcagtacac tggcaatcga tatgaaacgc aggcattacc gtcaggtaga cgtcatcgtc
      961 acgcagtgca cattgccagg cggagtaata tccagcgaag cgcaggttgt aatgggtaat
     1021 caccacccct ttcggtcggg aggtggtgcc ggaggtgaag agaatttccg ccgtatcgtc
     1081 agtcgatagc ggcggtgcat agcacaaggt ggcaggttgt tgatttttca gttgagtaaa
     1141 cgaactcacg ccatcatcag cgggaagtgc catatctatc aggcaaatgt gccgcaactg
     1201 agaggcatct tcctgctgaa tctgttgata cataggataa aattgcgcac tggtcaccag
     1261 caggcacgcc tggctatttt gcaggatcca cgcgctttcc tcgcgcaaca ggcgggcgtt
     1321 aattggcacc ataatcgcgc caatttttgc cagcccgaac cagcaaaaga taaattccgg
     1381 gcagttgtcg agatgtagag caaccttgtc gcctttgcga atccccagcg cataaaacag
     1441 gtttgccgtg cggttaatct cctgatttaa ctcaagataa ctataccggt taacgactcc
     1501 gctgctggat tcacaaatca gcgccgtttt atgaccgtaa acgtccgcca gatcgtccca
     1561 catttgacgt agatgttgtc cgccaatgat atccattgca cctctatcca ttgttgttcg
     1621 tttgttattg ggcgggcgct agtcaggcaa gccgactgac gccacgcgtt tagtcctcaa
     1681 ctttggccag acctttactg accaactcct gaatgtcgtt ttcgctgtag ccgatatttt
     1741 tcaaaatggc agccgtgtcc atgccatgtg agggcattcc gcgccagatt tttccggggt
     1801 tatttttgaa tttcggcatg atattcggcc ccttgcaggt gcgaccatcc atcgtttgcc
     1861 actgagtgat tgattcgcga gccacatact gtggattgct ttccagttcc ggtacggtca
     1921 gtactttggc acaggcgata ttcagttcgg caaagcgttc tttaacttct gcgatggtat
     1981 gtgcagccag ccaggcatcg agtttctctt ctaccagtgg gccgtaaggg cattcgatac
     2041 ggtggataag ctgagtgcct tccgggattt ccgacgtacc gagcagatgt gcgaggccaa
     2101 tatctttaaa gcactcttca atttgagtaa tgcccaccag ttccatcacg atgtagccgt
     2161 cggcgcattt gtatagaccg caaccggcgt agtagggatc tttacctttg gtcatgcgcg
     2221 ggcacatttc gccgccgttg aagtaatcca tcatgaagta ctggctcata cgcagcatca
     2281 cttcatacat ggcgatgtcg atactttcgc ctttaccggt ttcacgcact ttatgcagtg
     2341 ctgccagcgc cgccgtggtg gcggtcagac cagaaaagta atcggcggta tacgggaagg
     2401 caggcattgg ctggtcaaca tcaccgttct gaatcaggta accactgaag gcctgggcga
     2461 tggtgttata ggccggaaga ttggtgtact cctcggtgcc gtactgacca aaaccggaca
     2521 ggtgagcgat aaccagtttc gggttgtgct gccacaggac ttcatcggta atgccacgac
     2581 gggcaaaggc cggacctttg ctggcttcga tgaagatatc ggtggtttcc attaatttca
     2641 gaaacgcttc gcggccttca tctttgaaaa tatttaacga cagcgcgtgc aaattgcggc
     2701 gggagagctg cgggtagttc ggttgaacgc gaatggtgtc ggcccaggcg acgttctcga
     2761 tccaaataac ttccgcgccc cattctgcga acatttgccc ggcgaacggc ccggcgattt
     2821 cgataccgga gaagacaacg cgcaatccgg ccagcggccc gaatttcggc atgggtagat
     2881 gatccattat tagctcctga aaaatttatg tagcgcatga ctgccggatg cggcgtaaac
     2941 gctttatccg gcctacattc gtgctcccgt aggtctgata agacgcgtca gcgtcgcatc
     3001 aggcagcgca cggacttagc ggtattgctt cagcaccgcg cgacccagcg tcaggatctg
     3061 catttcgtca gatcccccgg agacgcggtc tacacgcaga tcacgccaga agcggctgat
     3121 gcggtggttg cccgcaatcc cgacaccgcc cagcacctgc attgcgctat ccaccacttc
     3181 aaatgccgca ttggcgcaga agtatttgca catcgctgca tcgccagagg tgatggtgcc
     3241 gttgtctgct ttccacgctg cttcatacag catgtttttc atggagttta atttgatcgc
     3301 catgtgggcg aatttttcct gaatcaactg gaaacgacca atagcctcgc caaactgcac
     3361 gcgctggttg gcgtagcgcg ccgcatcttc aaaggcgcac atcgccgtac cgtagttggt
     3421 cagggctacc aggaaacgtt catggtcgaa ctcttctttg acgcggttaa agccgttacc
     3481 ttcccgaccg aacatgtctt tctcgtccag ttccacgtcg tcaaaagtga tttcacagca
     3541 gctatccata cgcagaccga gcttttcaag tttggtcact ttgatgcccg gtttgctcat
     3601 atcaacaaac cattcggtgt agacaggttt gtccggagaa gccccgtcgc gcgccatcac
     3661 cacgatgtac ggggtgtagg cgctgctggt aataaaacac ttactaccat taagataaat
     3721 cttaccattt ctacgggtat aagtcgtttt caggctaccc acgtcggagc ccgcgcccgg
     3781 ttcggtaatc gctgagttcc acatctgctt accggtgccg cggaaagcca taattttgtc
     3841 gatctgctct tgtgtgcctt cgcgcaggaa ggtgttgaac ccgcccggca actggtacag
     3901 cacataggtt ggtgccccca gacgtcccag ctccatccac acggcggcga gagtaacaaa
     3961 ccccgcgtcc agaccaccgt gctcttcagg gatcagcaga ctgtcgatac ccatatccgc
     4021 cagtgctttg acaaaacgtt ccgggtagac gctgtcacgg tcgcactcgg caaaataggc
     4081 ctcccagttt tcgctggcca tcagttcgcg gataccggcg acaaacagtt cctgctcatc
     4141 atttaaatta aaatccatct ttcaacctct tgatattttg ggggttaatt aatctttcca
     4201 gttctgtttc gcgtctttaa taaaggagag cgtcaccata atgttgacga agaacagcgg
     4261 gcatcctccg gcgataatgg cagtttgaat cggtttcagg ccgccgagcg ccagcaaaac
     4321 aataccgata atgccaacca gaattgacca gccgatacgc accagcagcg gtggttcttc
     4381 accatcgcgt acttcgcggc aagtggacat cgccagggta taagagcagg cgttaaccag
     4441 cgtaacggtg gcaataaagc agaggatgaa gaagccccac atggtggcgg tgctgagtgg
     4501 cagagcggcc caggtttcga tgatggcgcg cgccacaccg tactgttcga tcagatttgg
     4561 aatgttgatg atgtttttat ccatcaacag cagagtgtta ctaccgagca cggtccacag
     4621 gatccaggtt gacgctgtca gccccatcac catgccgaag cacagttcac gcacagtacg
     4681 accacgggag atgcgggcga ggaagatact catctggata gcgtaaatca cccaccatgc
     4741 ccagtagaac acggtccagc cctgcgggaa gccgccttta gcgatgggat cggtatagaa
     4801 caacatgcgc ggcagataca tcagcaacat ccccaccgaa tcggtgaagt agttcatgat
     4861 gaagctggca ccgctgacaa tgaacaccca acccagcatc aggaagctca ggtaactacg
     4921 cacgtcactg gcgatacgta cccctttttg cagaccgcaa gcgacgcaaa tggcgttgag
     4981 gataatccag caggtaatga tgatagcgtc cagttgcagg gtatgcggaa tgccaaacaa
     5041 ccattgcata cactcggtca ccagcggcgt ggcaaggccc agactggtac ccatcgcgaa
     5101 gatcaaggcg acgagataga agttgtcgac gatagtgccg aacaaccctt tggcgtgttt
     5161 ttcacctacc agcggcacta gtgtcgagct ggggcgaatc acttccattt tgcggacaaa
     5221 gaagaagtaa gcgaaggcga ctgaaaggaa gctgtaagtg gcccacggca gaggtcccca
     5281 gtggaacaag ctgtaagcca gccccaactc tttcgcccct gtcgagttcg gttctaagcc
     5341 aaacggcggg gtggagatgt agtagtagat ctcaatcgat ccccagaaca gtacggcagc
     5401 agacgtacag gaggcgaaca tcataaagat ccaactggcg gtactaaatt caggtggttc
     5461 gttacctaaa cgctttttgg catacgggcc aaacaccagc cagaaccaac cgaaaagcat
     5521 cactaccata taccattcaa atgcccatcc ccatacattg gtgacgtaac tgaatacagc
     5581 attaataacg acattcgctg catccagatc tctgactgta agccaacaaa gtatgccgac
     5641 gattattaac ggcggaaaga aaaccttc
//
LOCUS       DACBVI010000220         5657 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.222, whole genome shotgun sequence.
ACCESSION   DACBVI010000220 DACBVI010000000
VERSION     DACBVI010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.222"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            40..582
                     /gene="fimA"
                     /locus_tag="IU325_21350"
     CDS             40..582
                     /gene="fimA"
                     /locus_tag="IU325_21350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein subunit FimA"
                     /protein_id="HAP5811541.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            802..1494
                     /gene="fimC"
                     /locus_tag="IU325_21355"
     CDS             802..1494
                     /gene="fimC"
                     /locus_tag="IU325_21355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HAP5811542.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            1525..4134
                     /locus_tag="IU325_21360"
     CDS             1525..4134
                     /locus_tag="IU325_21360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HAP5811543.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTVNTGDKSGGLMP
                     CFNQALLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNAYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            4147..5154
                     /gene="fimH"
                     /locus_tag="IU325_21365"
     CDS             4147..5154
                     /gene="fimH"
                     /locus_tag="IU325_21365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HAP5811544.1"
                     /translation="MKIICRLLLAMACLCLTNISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPNDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            5165..>5657
                     /gene="sfmF"
                     /locus_tag="IU325_21370"
     CDS             5165..>5657
                     /gene="sfmF"
                     /locus_tag="IU325_21370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HAP5811545.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAVDPLGTININLHGNVVDFSCTVNT
                     ADKDKTVDLGRWPTTQLLSAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDANLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASQTEEVDANGNVTLNFFANYRALASGVQPGVA
                     KADA"
BASE COUNT         1484 a         1224 c         1376 g         1573 t
ORIGIN      
        1 acggatgtgg agtcgataaa tgagattgaa ggaatatata tgaaattaag atttatttcg
       61 tctgcgctgg ccgccgcact attcgccgct acgggtagtt atgctgccgt tgtagatggc
      121 ggtacaattc actttgaagg cgaactggtg aatgctgcct gttcagtgaa tactgactcg
      181 gcagaccagg ttgtcacact cggtcaatat cgtaccgata ttttcaatgc tgttggtaat
      241 acctctgcat taattccatt cactatacag ttgaacgact gcgatcctgt cgttgccgct
      301 aatgctgccg ttgcattttc tggtcaggcg gatgcaatca atgataattt attggccatt
      361 gcatccagta ctaatacaac aacagcaacg ggtgtcggta ttgaaatact tgataataca
      421 tccgcaattc tcaaacctga tgggaatagc ttctcaacca accagaactt gatccccggg
      481 accaacgttc ttcatttttc tgcacgttat aaaggcaccg gtacaagtgc atcagcaggg
      541 caagcaaatg ctgacgcgac ttttattatg agatacgaat aatcaaaacc acgttgtttt
      601 gaattatata tcacgtctta taacaaagta atgtaccggt tgtctgaagc ggtatggtgg
      661 caatgtaaat cgaaatcatg ttcactttgt atcatgccgc tttattaaat gaaaagggaa
      721 tgatgtgttg taagaaacca aagcaatcat ttctttatat ttcttatttt tgccgtcagg
      781 aatacacaag gcgtattaac tatgatgact aaaataaagt tattgatgct cattatattt
      841 tatttaatca tttcggccag cgcccatgct gccggaggga tcgcattagg tgccacgcgt
      901 attatttatc ccgctgatgc taaacagact gcggtatgga ttagaaatag ccataccaat
      961 gagcgctttc tggtcaattc gtggattgaa aacagcagcg gtgtaaaaga aaagtcattc
     1021 atcattacac cgccactgtt tgttagtgaa cccaaaagcg aaaatacttt gcgtattatt
     1081 tacaccggtc caccgctggc ggcagatcgt gagtctctgt tctggatgaa tgttaagacg
     1141 atcccttcgg tagataaaaa tgcattgaac ggcaggaatg ttttgcaact ggcgatttta
     1201 tcgcgcatga aattatttct ccgtccaatt caattacaag aattacccgc agaagcgccg
     1261 gacacactca agttttcgcg atccggtaac tatatcaatg ttcataatcc atcacctttt
     1321 tatgtcaccc tggttaactt gcaagtgggc agccaaaaac tggggaatgc tatggctgca
     1381 cccagagtta attcacaaat tcccttaccc tcaggagtgc agggaaagct gaaatttcag
     1441 accgttaatg attatggttc agtaaccccg gtcagagaag tgaacttaaa ctaaccgaat
     1501 catctgacaa tatcagagct aattatgaaa atacccacta ctacggatat tccgcagagg
     1561 tatacctggt gtctggccgg aatttgttat tcatctcttg ccattttacc ctccttttta
     1621 agctatgcgg aaagttattt caacccggca tttttattag agaatggcac atccgttgct
     1681 gatttatcgc gctttgagag aggtaatcat caacctgcgg gcgtgtatcg ggtggatctc
     1741 tggcgtaatg atgagttcat tggttcacag gatatcgtat ttgaatcgac gacagtaaat
     1801 acaggtgata aatcaggtgg gttaatgcct tgttttaatc aggcactcct tgaacgaatt
     1861 ggccttaata gcagtgcatt ccccgagtta gcccagcagc aaaataataa atgcattaat
     1921 ttactgaaag ctgtacctga tgccacaatt aactttgatt ttgcagcgat gcgcctgaac
     1981 atcactattc ctcagatagc gttgttgagt agcgctcacg gttacattcc gcctgaagag
     2041 tgggatgaag gtattcctgc tttactcctg aattataatt tcaccggtaa cagaggtaat
     2101 ggtaacgata gctatttttt tagtgaactc agcgggatta atattggccc gtggcgatta
     2161 cgcaacaatg gttcctggaa ctattttcgc ggaaatgcat atcattcaga acagtggaat
     2221 aatattggca cctgggtaca gcgcgccatt attccgctga aaagtgaact ggtaatggga
     2281 gacggcaata caggaagtga tattttcgat ggcgttggat ttcgtggtgt acggctttac
     2341 tcttccgata atatgtatcc tgatagccag caagggtttg ccccaacggt acgtgggatt
     2401 gcccgtacgg cggcccagct aacgattcgg caaaatggtt ttattatcta tcaaagctat
     2461 gtttcccccg gcgcttttga aattacagat ttgcacccga catcttcaaa tggcgatctg
     2521 gacgtcacca tcgacgagcg cgatggtaat cagcagaatt acacgattcc gtattcaaca
     2581 gtgccaattt tacaacgcga agggcgtttc aaatttgacc tgacggcggg cgattttcgt
     2641 agcggtaata gtcagcaatc gtcgcctttc ttttttcagg gcacggcact cggcggttta
     2701 ccacaggaat ttactgccta cggcgggacg caattatctg caaattacac cgccttttta
     2761 ttagggctgg ggcgcaatct cgggaactgg ggcgcagtgt cgctggatgt aacgcatgcg
     2821 cgcagtcagt tagccgacga cagtcgtcat gagggggatt ccattcgctt cctctatgcg
     2881 aaatcaatga acactttcgg caccaatttt cagttaatgg gttaccgcta ttcgacacaa
     2941 ggtttttata cccttgatga tgttgcgtat cgtcgtatgg aggggtacga atatgattac
     3001 gattatgacg gtgagcatcg cgatgagccg ataatcgtga attaccacaa tttacgcttt
     3061 agtcgtaaag accgtttgca gttaaatatt tcacaatcac ttaatgactt tggctcgctt
     3121 tatatttctg gtacccatca aaaatactgg aatacttcgg attcagatac ctggtatcag
     3181 gtggggtata ccagcagctg ggttggcatc agttattcac tctcattttc gtggaatgaa
     3241 tctgtaggga tccccgataa cgaacgtatt gtcggactta atgtttcagt gcctttcaat
     3301 gttctgacca aacgtcgcta cacccgggaa aatgcgctcg accgcgctta tgcctctttt
     3361 aacgccaacc gtaacagcaa cgggcaaaat agctggctgg caggcgttgg tgggacctta
     3421 ctggaaggcc acaacctgag ttatcacgta agccagggcg atacctcgaa taatgggtat
     3481 acgggcagcg ccacggcaaa ctggcaggcc gcttacggta cgctgggggt cgggtataac
     3541 tacgaccgcg atcaacatga cgttaactgg cagctgtctg gcggtgtggt cgggcatgaa
     3601 aatggtataa cgctgagcca gcctttaggg gataccaatg ttttgattaa agcgcctggc
     3661 gcaggcggtg tacgcattga aaatcaaact ggcattttaa ccgactggcg cggctatgcg
     3721 gtgatgccgt atgccacggt ttatcggtat aaccgtatcg cgcttgatac caatacgatg
     3781 gggaattcca tcgatgttga aaaaaatatt agcagcgttg tgccgacgca aggcgcgttg
     3841 gttcgtgcca attttgatac ccgcataggc gtgcgggcgc tcattaccgt tacccagggc
     3901 ggaaaaccgg tgccgtttgg atcactggta cgggaaaaca gtaccggaat aaccagtatg
     3961 gtgggtgatg acgggcaagt ttatttaagc ggtgcgccat tgtctggtga attactggtt
     4021 cagtggggag acggcgcgaa ctcacgctgc attgcgcact atgtattgcc gaagcaaagc
     4081 ttacagcaag ccgtcactgt tatttcggca gtttgcacac atcctggctc ataaaggaaa
     4141 ttatcaatga agataatctg cagattatta ttggcaatgg catgtttgtg tctgacaaac
     4201 atatcctggg ctactgtttg tgcaaatagt actggcgtag cagaagatga acactatgat
     4261 ctctcaaatg tctttaatag caccaataac cagccagggc agattgttgt tttaccggaa
     4321 aaatccggct gggtaggtgt gtcagcaatt tgtccacccg gcacgctggt gaattataca
     4381 taccgtagtt atgtcaccaa ctttattgtt caggaaacta tcgataatta taaatatatg
     4441 caattaaatg attatctatt aggtgcgatg agtctggttg atagcgtgat ggatattcaa
     4501 ttccctccgc aaaattatat tcgtatggga acagatccta acgtttcgca aaaccttccg
     4561 ttcggggtga tggattctcg tttgatattt cgtttaaagg ttattcgtcc ctttattaac
     4621 atggtggaga tccccagaca ggtgatgttt accgtgtatg tgacatcaac gcctaacgat
     4681 ccgttggtta cacctgttta taccattagt tttggtggcc gggttgaagt accgcaaaac
     4741 tgcgaattaa atgccgggca aattgttgaa tttgattttg gtgatatcgg cgcatcgtta
     4801 tttagtgcgg cagggccggg gaatcgacca gctggtgtca tgccgcaaac caagagcatt
     4861 gcgatcaaat gtacgaatgt tgctgcgcag gcttatttaa caatgcgtct agaagccagt
     4921 gccgtttctg gtcaggcgat ggtgtcggac aatcaggatt taggttttat tgtggccgat
     4981 cagaacgata cgccgatcac gcctaacgat ctcaatagcg ttattccttt ccgtctggat
     5041 gcagctgcgg cagccaatgt cacacttcgc gcctggccta tcagtattac cggtcaaaaa
     5101 ccgaccgaag ggccgtttag cgcgctgggg tatttacgcg tcgattatca atgaggtacg
     5161 gagaatgaga agagtactct ttagctgttt ctgcgggcta ctgtggagtt ccagtggatg
     5221 ggcagttgac cctttaggaa cgattaatat caatttgcac ggtaacgttg ttgatttctc
     5281 ctgtaccgta aacacagcgg ataaggataa gacggtagat ttaggcagat ggcctacgac
     5341 acaattactg agcgctggcg ataccacggc actcgtccct tttagcctgc ggctggaggg
     5401 atgtcctccg ggttcagttg cgattttatt tacgggaacg ccggcatctg atgccaacct
     5461 gctggctctg gatgatcccg caatggcaca aaccgtcgcc atcgaattac gtaatagcga
     5521 tcgctcccgg ctcgcactgg gggaggcgag ccagactgag gaagtagatg caaatggcaa
     5581 tgtcacacta aacttttttg ccaattatcg agcgttagcc agcggtgttc agccaggtgt
     5641 ggcgaaagcg gatgcga
//
LOCUS       DACBVI010000221         5617 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.223, whole genome shotgun sequence.
ACCESSION   DACBVI010000221 DACBVI010000000
VERSION     DACBVI010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.223"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            120..1478
                     /gene="mltD"
                     /locus_tag="IU325_21375"
     CDS             120..1478
                     /gene="mltD"
                     /locus_tag="IU325_21375"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HAP5811546.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1525..2295)
                     /locus_tag="IU325_21380"
     CDS             complement(1525..2295)
                     /locus_tag="IU325_21380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP5811547.1"
                     /translation="MTSQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYVE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDVWL
                     QTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVKTYFALQNDGSFTSDIIIVEAHKAA"
     gene            complement(2373..3173)
                     /locus_tag="IU325_21385"
     CDS             complement(2373..3173)
                     /locus_tag="IU325_21385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP5811548.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3414..4328
                     /locus_tag="IU325_21390"
     CDS             3414..4328
                     /locus_tag="IU325_21390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP5811549.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4325..5128)
                     /gene="dkgB"
                     /locus_tag="IU325_21395"
     CDS             complement(4325..5128)
                     /gene="dkgB"
                     /locus_tag="IU325_21395"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HAP5811550.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLQESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(5290..5366)
                     /locus_tag="IU325_21400"
     tRNA            complement(5290..5366)
                     /locus_tag="IU325_21400"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5330..5332),aa:Asp,seq:gtc)
     gene            complement(5421..5536)
                     /gene="rrf"
                     /locus_tag="IU325_21405"
     rRNA            complement(5421..5536)
                     /gene="rrf"
                     /locus_tag="IU325_21405"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1458 a         1370 c         1398 g         1391 t
ORIGIN      
        1 cctgaagaac gttttgcatg gttaaggtca aagaaagata ggttctgata aaactttctt
       61 gtcatcggct ccgttcgccg ttatgatagg tcgtctttta agcaactatt gacacacaca
      121 tgaaggcaaa agcgatatta ctcgcctctg tcctgctcgt gggttgccag agtaccggca
      181 acgttcaaca gcacgcacag agcctttctg cagctggtca aggggaagca gcaaagttta
      241 caagtcaggc acgatggatg gacgatggga cgtctatcgc gccagatggt gacttgtggg
      301 ctttcattgg cgacgagcta aagatgggaa ttccggaaaa tgaccggatt cgcgaacaga
      361 aacagaaata tttacgcaat aagagctatc tccacgatgt aactttacgg gcagagccgt
      421 atatgtactg gatagccggg caagttaaaa aacgtaacat gcctatggaa ctggtactac
      481 tacccatagt ggagagcgct tttgatcctc acgcaacgtc tggcgccaat gcagcaggca
      541 tctggcagat cattccgagc acggggcgca attatggttt gaaacagacc cgcaattatg
      601 acgcgcgtcg cgatgttgtt gcttcaacaa ctgccgcgct gaacatgatg cagcgtctga
      661 acaagatgtt tgacggcgac tggcttctga ccgtagcggc ttataacagc ggcgaaggtc
      721 gagtcatgaa ggcaattaaa acgaacaaag cgcgtgggaa atccacggac ttctggtcgt
      781 taccgttgcc gcaggaaacg aagcagtacg tgcctaaaat gctggcattg agtgatattc
      841 tcaaaaacag caagcgttat ggcgtacgtc tgccaacgac cgatgaaagc cgtgctctgg
      901 cgcgtgtgca cctgagcagc ccagttgaaa tggcgaaggt tgcagatatg gcggggattt
      961 ccgtcagcaa gcttaagaca ttcaacgctg gcgtgaaagg ctccacgctg ggcgcaagtg
     1021 gtccgcagta cgtgatggtg ccaaagaagc atgcagatca actgcgtgaa tctctggctt
     1081 cgggcgaaat tgctgctgta cagtcgacgc tggttgccga caatacgccg cttaacagcc
     1141 gtgtttacac cgtacgctct ggcgacacgc tttcaagtat cgcttcacgt ctcggcgtta
     1201 gcaccaaaga tttgcagcag tggaacaaac tgcgcggatc taagctgaag ccaggccaaa
     1261 gcttgacgat tggcgcaggt agtagcgcac agcggttggc aaacaacagc gatagcatta
     1321 catatcgtgt gcgcaaaggc gattcgcttt caagcattgc taaacgccac ggcgtgaaca
     1381 tcaaagatgt gatgcgctgg aacagcgata ctgcgaatct gcaaccaggc gataagctga
     1441 cgttgtttgt gaaaaacaac aacatgccag attcctgaca aatcagataa tgaaaggcac
     1501 cgattccccc ggtgcctttt ttatttatgc tgctttatgt gcttctacaa tgatgatatc
     1561 actggtgaaa gagccatcat tctgcaaggc aaaatacgtt ttcacctctg tcgatgcgct
     1621 ctgttggtaa atgcgaatag cgtctactaa cgcttctggc gtacgcattc tcgcgaccca
     1681 tgaagaaaat tccagcggta acttatctgt aattaaatta tcaactatca gattggcttc
     1741 attgattaac gtcaaccact caccgctggc gtagtttcgt acgtgagagg tatcgcgtaa
     1801 tgcttctacc gtctgtaacc agacgtcgcg cactgggtgc cccggagaca ttacgtccat
     1861 cacaatcagc ctaccgccag gtttcaatat cctattcact tctcgcagtg ctgcaccaac
     1921 atcatgccag tgatgggcag aataacggct gataacaata tcaaatgcgt tatcggcaaa
     1981 tggcagactt tcgacataac cctggcgggt ggtgatattt ttcagttgcc gggcttcggc
     2041 agcttgtgcc acgacatcca gcatttgggc agataagtca tacgccacca ccgcgctcac
     2101 gttctgcgca gcgacaaagc tggcatgccc tgctccgcag cccatatcaa gtacacttgc
     2161 atcaggataa tcagccagac gcaccgccag gcgctgtaaa tcccgtccgg atgcatgtac
     2221 ggtactagtc agatattcgc tagcttgaga gctaaactgc ttttctacgt ggtcatggtg
     2281 ggattgtgat gtcattgcgc tgtccttttg ttgtttgagt ataaaagggc agcgccaaac
     2341 gttggtctgc cctgtggcag acctgacata ccttatttat caggcttgcc gggactgaat
     2401 tcaacgagta gcggattgtg atcggaagcg cgcgtaacca gtacagaagc ttcgctgacg
     2461 ttcagaccac ggtaaaaaac aaaatcgagc gggcgaccaa acgcccgacg gcgctgatcg
     2521 tcggtaaaac gcacctggcg cagcgacatt tcccgcgcaa agcgatataa cgcgttcatc
     2581 cttctacggc tccaggcatt gaaatctccc gccataatga ccgggccgct gtgatgagct
     2641 atctgatcgc caataggaag taactgctta ctataaacat ccacgcccag actgaagttg
     2701 acggcgtgta tattaaccac catcaacagg cgggtgtcag gtaatggata gaccgtcacc
     2761 agtgccgact tcgccagacg caaaatgggt tctcgttcac gtaacgggca gcaatacact
     2821 ggatgtgccg ccgaaagggt cattacgcca gaaggatgtt gtggcagcac gaaagcgggt
     2881 acctgatcgg cggcaagata gttagcggtc gcaaactgta ctaactctgg cgttgtctgc
     2941 gcttcctgca ataacaccag atgtgcatct ttgccgtagt tctttaatac cgacaaccat
     3001 tcagcgcgtt gctgtttgta tatgttccac accaggatcc gaatacgctc ttcggtactt
     3061 aacggttctc cgggtggtaa tgcctggccg atgctcgcaa aagaccccgg cggtaagatc
     3121 ctttccgcag gttgtccggc aacatagcgc atggcatagg tgttttttcg cacttttgac
     3181 tttgaaacct ctattacgcc aacccttccg cactcgggag ggttctactg ttatagggat
     3241 tttagctcac tctttcaacg agcaattgta tattgttatg taagcaagtg cttacaccgg
     3301 tcagaaattt gctcattatt gagaacatta aatcggatga catatcacgc atcctgatgg
     3361 tttaatgaca aataaattca aaatatctca tctattttgc aacaggagca aaaatgaaag
     3421 ccacgtcgga agaactcgcc atttttgttt cggtcgtcga aagcggcagc tttagccggg
     3481 cagcggaaca attagggcaa gcaaactcag cggtaagccg ggcggtgaag aagctggaga
     3541 tgaaacttgg cgttagcctg cttaatcgga ccacgcggca acttagcctg acggaagaag
     3601 gcgagcgtta ttttcgtcgc gtacagtcaa ttttgcagga gatggcagcg gcagaatcag
     3661 aaattatgga gacgcgtaat acaccgcgtg gactgttgcg catcgatgcc gcaactccag
     3721 tagtgctgca ctttctgatg ccgttgatta agcctttccg tgaacgctat ccggaagtca
     3781 ctttatcact agtctcctcc gaaacgatta ttaatttgat cgaaagaaaa gtggatgtcg
     3841 cgatacgcgc cggtacgtta acggattcca gcttacgtgc caggccgtta tttaacagtt
     3901 atcgaaaaat tatagcctcc cccgattata tttcccgcta tgggaagcca gaaacgatcg
     3961 acgatttaaa gcaacatgtt tgcctgggat tcactgaacc cgcttccctc aatacctggc
     4021 cgatagcctg tagcgatgga caattacatg aggtgaagta cggtttgtca tccaatagtg
     4081 gggaaacact gaaacagctt tgcctgagtg gcaacgggat tgcgtgtttg tccgactaca
     4141 tgatcgacag agaaatcgct cgcggagaat tggtggagct aatggcagat aaagtgttgc
     4201 cagtggaaat gccattcagt gcagtctatt acagcgaccg tgcggtaagt acgcgcatcc
     4261 gggcttttat cgatttcctt agcgagcatg taaaaacagc tcccggagga gctgtcagag
     4321 aggcttaatc ccattcagga gccagacctt ccgggctaac caggcggtcg ttgcaatcca
     4381 gtgcggcgat cgctttttta tcttcggcat caagctgtaa attttgtgcc ttaagattac
     4441 tttccaggtt tttacgttta gtagaagaag gaattactga gtaaccttcc cccatagccc
     4501 acgccagaat cacttgtgcc ggagtcgcat tgtgtttagc tgcgatacga gcaataacct
     4561 catctttcag ggccttacca tacgccagcg tcatatagga agtaatatgg atgccgtgct
     4621 gtttagccca ggcaaccact ttacggtttt gcagataagg agagagttca atctggttag
     4681 tagcgatgtt ttcagcacca acagcagcaa tcgctttttc catcaatggg atcgtgaagt
     4741 tggaaatacc gatctcacgc gtcagccctt gttttttggc ttccagcagc gcctgcataa
     4801 actcttcaac agagacttca tcgtttggtg acggccagtg gattagcgtc agatcaacat
     4861 aatcagtacg caatttttgc aggctctctt gcagactcgg gatcaatttg tctttgctga
     4921 gattttcaat ccagatttta gtggtgatgt agagttcatg acgtggcacg ccactttctg
     4981 caatcgcctg acctacggca gcttcgttat catagatttg tgcggtatca attgcgcgat
     5041 aaccaagttc aagcgccgtt ttcacagatg aaataacaac gtcgtctttg agacggaaag
     5101 tacctaaacc aaatgcaggg atagccataa tatgcctctt ttaatgctat tttcgttaac
     5161 tgaggtgatt atgggcttca gactgttgcg gaaaaagagc gtaaaatgca gaggattttt
     5221 gcgattctgg caataataga tatacagacg aaaaaaacct cgagcgttaa ctcgaggctt
     5281 cttaataagt ggcggaacgg acgggactcg aacccgcgac cccctgcgtg acaggcaggt
     5341 attctaaccg actgaactac cgctccaccg aattctttta caaccaccgg ttttatgacc
     5401 ggcttactgc taaatttgat gcctggcagt tccctactct cgcatgggga gaccccacac
     5461 taccatcggc gctacggcgt ttcacttctg agttcggcat ggggtcaggt gggaccaccg
     5521 cgctaaggcc gccaggcaaa ttctgtttca tcagaccgct tctgcgttct gatttaatct
     5581 gtatcaggct gaaaatcttc tctcatccgc caaaaca
//
LOCUS       DACBVI010000222         5578 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.224, whole genome shotgun sequence.
ACCESSION   DACBVI010000222 DACBVI010000000
VERSION     DACBVI010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.224"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..660
                     /locus_tag="IU325_21410"
     CDS             <1..660
                     /locus_tag="IU325_21410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HAP5811551.1"
                     /translation="INKMRVVALTPALQPIDGVAVSYIDAAVALGNTINEMDKYYTQE
                     NYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAELKNIEQREGKTF
                     HYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKGGMNFSFINSA
                     DQYQLEAKKYVRRVRDKVPYSDWDKEQLQDANTSWMVDDSFPRALREYNEMVDDYNSL
                     R"
     gene            complement(716..2464)
                     /locus_tag="IU325_21415"
     CDS             complement(716..2464)
                     /locus_tag="IU325_21415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP5811552.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGNAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERGFCLASRPGLGHFIDETEEKRIILLANLLRKDPFLIPLA
                     GVTRDNLQQLSTVCDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGSEIKQIV
                     EYSGLFLGDNAVRTLTGLIEKQHQQAQVICVDNVLALLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLNCRVIIARSLSDITAISQE
                     ITPLLIVNNSHYLLDDAVKNSITIKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHFSNVRGENWQHITQQICAQLVTQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVVNHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLKGYEAFMELLHKG"
     gene            complement(2464..3534)
                     /locus_tag="IU325_21420"
     CDS             complement(2464..3534)
                     /locus_tag="IU325_21420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP5811553.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSILNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAEKRGVEIGNFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVVALKSCATH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKINAFSQFRQVD"
     gene            complement(3524..4975)
                     /locus_tag="IU325_21425"
     CDS             complement(3524..4975)
                     /locus_tag="IU325_21425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HAP5811554.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGIENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDHIFSE
                     LPTNSQFFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTQQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELAHEH"
     gene            complement(4986..5432)
                     /locus_tag="IU325_21430"
     CDS             complement(4986..5432)
                     /locus_tag="IU325_21430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP5811555.1"
                     /translation="MAALTASCIDLNIQGNGTYSVLKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGIAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFINQLRQGDADQVLALLNQTLSS"
BASE COUNT         1432 a         1410 c         1433 g         1303 t
ORIGIN      
        1 ataaacaaaa tgcgtgtagt ggcgttaacg cctgcgctgc aacctattga tggcgtggcg
       61 gtgagttata ttgatgctgc cgtggcgttg ggtaatacca ttaacgaaat ggataaatat
      121 tacacccagg aaaattataa agacgatgcg tttgcgaaag ggaaaacgct gcaccagaca
      181 ttcttaaaaa atcttgaagc ctttgaacct gtagcagaat cttatcatgc ggcgattcag
      241 gaaattaacg ataagcgcca gcttgcggaa ctgaaaaata ttgaacaaag agaagggaaa
      301 acattccact actactctct ggcagtcatg atttcagcga aacaaattaa taacctgatt
      361 tcgcaagaga agtttgatgt tgatgcggca atgaagaaag tctctgaact ggaaacgctg
      421 gtggcgcagg ccaaagaagc ggataaaggc ggtatgaatt tctcgttcat taattcggca
      481 gaccagtatc aactcgaggc taaaaaatat gttcgccgcg tcagagataa agttccgtac
      541 tctgactggg ataaagagca acttcaggat gcaaacacca gctggatggt cgacgactca
      601 tttccgcgag cgttacgcga gtacaacgaa atggtcgatg actataatag cctgcgttaa
      661 acgtttttga ttttggtgat aaatgcagtg tcggatgcgg cgcatccgac acagttcacc
      721 ccttgtgaag taactccata aatgcctcat atccttttaa cccggcaata acctctgccg
      781 gatgctgaca caatacgctt gccagatagc taaatatttt tagctcatga cgcgcatcgg
      841 cggcggcaca ggcgatcagc acatggttaa ctacttcgtt attcacctca accggttggg
      901 cgagggtaat aaaaaatcca cgaaaacggc gttcctgttc gctccagcaa tgtgggatgg
      961 cgagacgatt aacaatcagg ttttcacctt cgccttcgcg ggcgatgatc cgttgtgctt
     1021 catcggcggt aatgtggtgt tgcgtcacta attgtgcaca tatttgctgg gtaatatgtt
     1081 gccagttttc accgcgtaca ttcgaaaaat gaaaacttcc gggggcagag aaaaaacgct
     1141 ccggctgctg gcgaataaat gccgtggcaa gaaaatgttt aatttgctca atcccggcgg
     1201 cggtaatgat atttttgatg gtaatactat tttttaccgc gtcatccagc aaatagtggc
     1261 tgttattgac gatcaataac ggcgtaatct cctggctaat ggcggtaata tcgctcaggc
     1321 tgcgggcaat aatcacccga caattcagga catcgcgctc aatggcgagc tgattaatag
     1381 tggcaatcgc gttctggtcc gagagcaaaa tgatcggctg gcgttcgttt tgatgtcgtt
     1441 ccagcgcaca ggcaaaatac aaaccgatca gatcgctgtc gaaaagggga atatcgagct
     1501 gttcacgcag tagcgtaata aagtgcagac tcatatcaaa ggccgcaggc caggcggctt
     1561 tcaggttatt catgctgctt tggcggtgct cagcaatcca tactggtgcg gcaaggcaac
     1621 gtaataaatg cccggtaata ttctcaacca gttgcgtatc aataatattc aatgacgcaa
     1681 tgcccggcac cctttgcagc aatgccagca cattatcgac acaaataacc tgcgcctgtt
     1741 gatgctgttt ttctatcaac cccgtcaacg ttcttacagc gttatcacca agaaacaggc
     1801 cgctatattc aacgatctgt tttatctcgc taccgggata ttgcggccac tcatcggtca
     1861 gttgattacg cagggcgtaa atcgccagaa tcaggctgga gagataatca ccctgcatca
     1921 gcggccagcg gtgttggttg tcgcagaccg tggataattg ctgaaggttg tctcgtgtta
     1981 cgcccgccag aggaattaaa aacggatctt tgcgtaaaag attcgccagc aaaataatgc
     2041 gtttctcttc tgtctcatca atgaaatggc ccaaaccagg gcgactagcg agacaaaaac
     2101 cgcgttcata gcgctgtttc aggcggggca ggcgctctgc tacccacgtt tctggcaaat
     2161 taagcgccga ggcgagttgc gcacggggag tgaaggtatt cagtaatagc agcgccagca
     2221 gccgatcgtc gttatcgtgc ttttgcagca actgaaaaaa gctgcggcgc tcgaagattt
     2281 ccagctgata gcccgcgttg ccactggcgg aaatgcgggc tttgccgtta agggtaaagt
     2341 tgagatagtc aatgtcgcgc aggatggtcc tgcccgaaac gccagtctgt tgtgccagct
     2401 cgccaggcgt gcgcggctgt tgctccagca gatcgacaat ctttaactgg cgttcgttta
     2461 gcattaatcc acctgacgga actggctaaa cgcgttgatt ttctccgcag ttaaggtcgt
     2521 cagaaaaccc cgtatcagcg tgagcagagc atcgtaatcg gcttttgaaa tcatgccgct
     2581 gttggcgtgc agataacgcg ttggcaggca cagcgcgaca accggacgcc cgccgcccat
     2641 cacgttgtag cgcccgccat ccgtcgcacc ggttttcatg gtggaaaatt gcagcggtaa
     2701 atcgttatgt gtggcacagc tttttaacgc caccaccagc ttctggttgg ggaagtagcg
     2761 tttatcaaac agcatcagcc ccggcccctg gcccagtttt agcgggtatt taatgttatc
     2821 aatgcccgga acatcgcctg cgacggcggt atccagcacg atcacaacat ccggtttaat
     2881 gtgttcagcc gaggtttgcg ccccgcgcag gccaacttct tcttccacac tgccaacgcc
     2941 atacagcgta atttcgggat tattcaccgt ctgcaatagc tcagccatca ttgcgcagcc
     3001 gatgcggtta tccaacgcct tgccgaccac tttatcttcg ccccagcagg caaaattggc
     3061 ttccgggcta ataaaattac caatttccac gccgcgtttt tccgcttctt cgcgactgtt
     3121 cgcgccaata tcaatgaaca tctcatcaaa tgacagcggt tgttgctttt gcttttccgt
     3181 taacgcatga ggcgcgacgg aaccaatcac accagggatt ttaaatccct tgtgtgtgcg
     3241 tatggttacc cggtggttga gtatcgactg attccaccag ccgccaatgg tggtaaaacg
     3301 cagaaaaccg ctctcgtcga tgtgggtgac cataaagccg acttcatcca tatgcccgac
     3361 aacggcaact tttggacctt tattcccctt acgggcaaca aagctgccca gaccatcaaa
     3421 ggtgatctca ttaacgcaag gttccagcgt gttgatcaga atgtcgcgaa cttcctgttc
     3481 atcgccgcta acggcgctgg cttcgcacaa ctgttgcagt aactcaatgt tcatgggcaa
     3541 gctcctgttg cgcggctttc gctttgcggc gtaaccataa tcctttcagg acgataatga
     3601 atgaaatgtt cagcgcgaga ccaatcgcca gcaccagata aaacgaaccc accggcgaca
     3661 tcaggccgat cagcgggtca aagataccca aaccaggtgc cagtcgtttg attccgaaag
     3721 cgatcaccag cacgccggta atgccgcccg ccagcgtatt ggcggtaatc attggcagtg
     3781 gtgccgccag agcgtaagga atagccggtt cggtcgcgac cgtggcaccg accacaatgg
     3841 cgctactggc ggcttctttt tcctgttgag taaacagctt gggcgcaatg aacgtcgcaa
     3901 gacccgccgc gacaggcggc atcagcgcga caacgccgac gatggcgtac cagtcgtaaa
     3961 tgtgtttttc cagcagtgag aagcagaaga accaggcggt tttattgata gggccgccca
     4021 tatcgaacgc cagcattgca ccgaccagaa acgccgccgc aaacttcatt gacggaggaa
     4081 tggtgttcag gaagtgcagc aacccgccca tgaggtcgga catcaccggt ccgataacgt
     4141 agtaagtcag tacgccaaaa accagcagag taacgaacgg gatcagcatg gaaccgagca
     4201 gcggttgtaa tgctttgcca aggcgcactt tacggaacca gaagacgaaa tagccaatcg
     4261 ccagcccaag caccaccgca ccgaggaagc ccgcgccaga ctgggtgccg agcagggctt
     4321 tgtcgttcgc cagatagcaa accagaaacg ccggagcgaa agcgggttta tcggcaatcg
     4381 acgaggcaat ataagcgccc atgatgggga tcataaaggt gaagccgaga taaccgatgg
     4441 attcaaccac ccaggtaaac gacggcgcgc ctttcgacat atcggtatac ggcaaaccga
     4501 actgcaccag catgttggca agcgccacca gaataccgcc accgatgaca aacggcagcg
     4561 ccgcagagac gcccgccatc aggtggctca ttacgctgcc actttgcact tcctgtttgc
     4621 cgagcttcac gccgctatct gcggcaaaaa attgtgagtt tgtcggtaat tcactgaaaa
     4681 tatggtcgat atttttcagc gcctgggaga tggcaatttc ataaaccttt ttcccggcaa
     4741 atcgcgcgcg atcatcaccg ctcaggccac gcccggtagc gagaatcacg taatcggcag
     4801 cggcaatctc ttcgctggat aagcggtttt cgatgccgct tgatccttga gtttcaactt
     4861 taatggtatg accgagagaa cgggctttct gttccagggc ttccgccacc atgtaggtgt
     4921 gagcgatccc ggcggggcag ttggtgatcg cgacaatacg taaggatgac tccatcgcca
     4981 cttccttatg agctgagggt ttgatttaac aaagcaagca cctggtcggc gtcgccctgt
     5041 cgcagttggt tgataaaatc ctggtggata attttgcgac acagtgtgcc gatgattttg
     5101 acctgatcct cttcgccact ttgcggcacg ccgaggcaga tccagcaatt gacgtcttcg
     5161 ccgtcgctgg ctttccagtc aatggcctgc gctttgcggg cgaataacac gaacggttgt
     5221 ttaacgcagg cgcttttacc gtgcggcacg gcgatgccgg aaccaaaacc ggtggagtgc
     5281 attttttcgc gcagcagcag agtttgcagg aactggtgtg agtcggtgat aaaaccgttt
     5341 tgtaacgcca ttgtcgccaa ctgcttcaga acggaataag tgccattgcc ctgaatattc
     5401 aggtcaatac agcttgcagt aagtgctgcc attattggat tccatgaaaa aatattatag
     5461 ttaattgtaa cggcggagcg gttttgtgat gagcgcggat atgtcgtgaa gtcgtgacaa
     5521 tataaggcag agtgaaggat aaattttgcg gagtaaggca catattgatg ggcaaaat
//
LOCUS       DACBVI010000223         5575 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.225, whole genome shotgun sequence.
ACCESSION   DACBVI010000223 DACBVI010000000
VERSION     DACBVI010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.225"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(52..2121)
                     /gene="glyS"
                     /locus_tag="IU325_21435"
     CDS             complement(52..2121)
                     /gene="glyS"
                     /locus_tag="IU325_21435"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP5811556.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDDADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPAVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAASLAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2131..3042)
                     /gene="glyQ"
                     /locus_tag="IU325_21440"
     CDS             complement(2131..3042)
                     /gene="glyQ"
                     /locus_tag="IU325_21440"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP5811557.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3137..3445)
                     /locus_tag="IU325_21445"
     CDS             complement(3137..3445)
                     /locus_tag="IU325_21445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811558.1"
                     /translation="MMMNSFFPAMALMVLMALVGCSTPPPVQKAQRVKVDPLRSLNME
                     ALCKDQAAKRYNTGEQKIDVTAFEQFQVSYEMRGYTFRKEQFVCSFDADGHFLHLSMR
                     "
     gene            3620..4615
                     /locus_tag="IU325_21450"
     CDS             3620..4615
                     /locus_tag="IU325_21450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAP5811559.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLLFYSAIALLYIALSTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWGSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAITLLTLVKNTLDTRTVRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4657..5094)
                     /locus_tag="IU325_21455"
     CDS             complement(4657..5094)
                     /locus_tag="IU325_21455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HAP5811560.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQITQEEYSE"
     gene            complement(5140..5481)
                     /locus_tag="IU325_21460"
     CDS             complement(5140..5481)
                     /locus_tag="IU325_21460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811561.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDDFFIHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSA"
BASE COUNT         1378 a         1440 c         1350 g         1407 t
ORIGIN      
        1 ttagcgataa aaaaagcctg ccagatggca ggctttttaa taacgacgtt attactgcaa
       61 cagcgaaata tccgcaactc gcaggaacag ttcgcgcagt ttctccagca tggtcagacg
      121 gttgagacgc aattctttgt catcaaccat gaccatcact ttatcgaaga aagcatcaac
      181 tggctcacgc agctctgcca gttcgaccag cgcatcctgg taacgacctt cagcaaagta
      241 cggctccagt ttatcgcgca gcacaaccac ctgcatcgcc agtttaattt cttccggctc
      301 tttcagagta gaagcattca cgcgatcgct cagcacttcg tcagacttcg ccagaatgtt
      361 agatacacgc ttgtttgccg ccgccagtga cgctgccgca tccagagtac ggaagtgcga
      421 tactgccttc atacgggcat caaaatctgc cggacgcgtc ggacgacgtg ccagtaccgc
      481 ctggattgtg tcaacggtgt aaccttcgtc ctgataccag gcgcggaagc gaccgagcat
      541 aaagtcgata acatcatcaa ccacgttggc attagtcagc ttatcgccat acagacgcac
      601 tgcttcttcg gtcagcgttt gcagatcaag gttgaggttc ttctcaacga taatacgcag
      661 tacgccaagt gcggcacgac gcaacgcaaa cgggtctttg tcgcctttcg gatgctggcc
      721 gataccgaag atacccgcca gagtatccat cttgtcagca atcgccagcg cacaggctac
      781 cgggttagac ggcaggtcat caccggcaaa gcgcggctga tactgctcgt tcagcgccac
      841 tgcaacatct tccgcttcgc catcgtgacg cgcgtagtgc atccccataa cgccctgggt
      901 gtcggtgaac tcgaagacca tgttggtcat caggtcgcac ttagacagca ggcccgcacg
      961 ggtagcatgg ttaacgtcag cgccaatctg ttcagcaatc cagccagcca gtgcctggat
     1021 gcggtcagtt ttgtcacgca gtgtacccag ctgttgctgg aacagcacgg tttgcaggcg
     1081 cggcaggtta tcttccagac gttttttacg gtcggtattg aagaagaact cagcatccgc
     1141 cagacgtgga cgaacgactt tctcgttacc ggagataatc tgctgcggat ctttcgattc
     1201 gatgttggca acgaagataa agttcggcag aagtttgccg tcgttcgcat acaccgggaa
     1261 gtacttctgg tcacctttca tggtgtaaac cagcgcttca gacggcaccg cgaggaattt
     1321 ctcttcgaat tttgcggtca gcacagccgg ccattccacc agcgaagcga cttcttccaa
     1381 caggctttcg cttaagtcag cgttaccgcc aatcttacgc gccgcttctt cagcatcggc
     1441 tttaatcttc gctttacgtt cttcgtaatc ggcgatgact ttaccgcgct cacgcagaat
     1501 ttccggatac tgatcggcat cgtcgatagt gaattccggc tcgcccataa agcggtggcc
     1561 gcgaatcacg cgatcggact gaatgcccag aatggtagcc ggaatgactt tgtcgcccag
     1621 cagcagggtc acggtgtgca ccggacgcac gaagtgcacg tcgcttgcgc cccagcgcat
     1681 cagtttcggg atcggcagtt tcgccagcga agtcgcaacc atattcggca gcagtgcttc
     1741 ggtgctttcg cccttcacgt gggcgcgata cagcagccat tcacctttat cggtagtcag
     1801 acgctcagcc tggtctacgg taataccgca accacgcgcc cagccttctg ccgctttgct
     1861 cggtttgcct tcagcgtcga acgcctgagc aatcgccggg ccgcgttttt cgatttcacg
     1921 atccggttgc gcttccgcca ggttagctac tttcagcgcc agacgacgcg gagcagcaaa
     1981 ccattgaacg gtgccgtgtg cgaggccggc gttatccagc tccgcagtga agttcgcagc
     2041 aaaggactca gccaggctgc gcagtgcttt tggtggcagc tcttcagtgc cgatttccac
     2101 cagaaaagtt ttctcagaca tagccgcctc ttacttatct ttgttgcaca tcgggaagcc
     2161 gagggcttca cgggaagcgt agtatgcttc agccactgct ttggtcaggg tgcgaattcg
     2221 cagaatgtag cgctgacgct cggtgacgga gatggcttta cgcgcatcca gcaggttgaa
     2281 gctgtgggcg gctttcagaa tacgctcgta ggctggcagc ggcagcggat tttccagcgc
     2341 cagcagctgc tgcgcttctt tctcgtactg ctcaaagcag gtgaacagga agtccacatc
     2401 cgcgtattcg aagttgtaag tggactgctc cacttcgttc tgatggaaca cgtcgccgta
     2461 ggtggtttta cccagcgggc cgtcgctcca gaccaggtcg taaacgctgt ctacgccctg
     2521 aatgtacatt gccagacgtt ccagaccgta ggtgatctcg ccggtaaccg gtttacactc
     2581 cagaccacca acctgctgga agtaagtgaa ctgcgtcact tccatgccgt tcagccacac
     2641 ttcccagccc agcccccagg cacccagcgt cgggttttcc cagttatctt ccacgaaacg
     2701 gatgtcgtgg atggtcgggt ccatgcccag ctctttcaga gaaccaaggt acagctcctg
     2761 aatattgtcc ggcgatggct taatgaccac ctggaactga tagtagtgct gtaaacggtt
     2821 ggggttttcg ccgtagcgac catcggtcgg gcgacgagaa ggctgaacat aagcagccgc
     2881 catcggttct ggccccagcg cgcgcagaca ggtcattggg tgagaggttc ccgcgccgac
     2941 ttccatgtcc aatggttgaa caatggtgca gccctggcga gcccagtaat cctgtaaggt
     3001 caggatcaag ccctggaagg tcctggtatc aaacttttgc atattatttc gtgctggata
     3061 cgtgtggatt taaaggaagg gatcagtata cccgctggat ggaagatata cagtacgaaa
     3121 cgggaaaaag cagggcttaa cgcatggaaa gatgcaaaaa atggccatct gcgtcaaaag
     3181 aacagacaaa ctgctcttta cggaaggtat aaccgcgcat ttcatagctt acctgaaatt
     3241 gttcaaaagc agtgacgtcg attttttgct cgccggtgtt ataacgtttt gccgcctgat
     3301 ccttgcataa cgcttccata ttcaacgaac gcagaggatc aacctttacc cgttgtgctt
     3361 tctgcacggg tggcggtgta gaacaaccca ctagcgccat aagcaccata agcgccattg
     3421 ccggaaagaa agagttcatc atcattaaat taccgtctgg tttgcaggtg agtcttatta
     3481 tttatatcgg tatagtggct gatatcttac gaatttcctt aagcctctat aagctgtagt
     3541 gataaaactc agattattcc agtgcgccaa ccgaatgatt tatcggtcag acttttttta
     3601 accagataca gagacacaga tgcagcccaa aatttactgg attgataacc tgcgagggat
     3661 agcgtgttta atggtggtga tgattcacac cactacctgg tatgtgacca atgctcatag
     3721 tgttagcccc gtcacctggg atatcgccaa tgttctgaat tctgcctctc gtgtcagcgt
     3781 gccgctattt ttcatgattt ccggctatct cttttttggc gaacgcagcg cccagccgcg
     3841 ccatttttta cgtatcggct tatgtctgct tttttatagc gcaatcgcac tgctctacat
     3901 tgcgctgtct acctccatca atgtggagtt agcgctgaaa aacctgctgc aaaagccagt
     3961 gttttaccac ttgtggtttt tcttcgcgat tgcggtgatt tatctggttt caccgctgat
     4021 tcaggtgaag aacgtcggcg gaaaaatgtt gctggtgcta atggtggtga ttggtattat
     4081 cgctaaccca aacacagtgc cgcagaaaat tgacggtttt gaatggctgc caattaactt
     4141 atatatcaat ggcgatactt tttactacat cctttatggc atgttgggcc gcgctatagg
     4201 gatgatggac acacagcata aagcactgtc gtgggggagc gccgcactgt ttgcgacggg
     4261 ggtttttatt atctctcgcg ggacattata tgaattgcag tggcgcggaa attttgccga
     4321 tacctggtat ctttactgtg ggccgatggt ttttatctgc gcaatcacgc tattgactct
     4381 ggttaaaaac acgcttgata cgcgtaccgt tcgcggactt ggcttaatct cccgccattc
     4441 gttgggtata tacggattcc acgccttgat tatccatgca ctgcgcaccc gggggattga
     4501 gcttaaaaac tggccaatac tcgatattat ttggatcttt tgcgcgacgt tggcagcgag
     4561 tttgttactt tctatgctgg tacaacgaat cgacagaaac agactagtga gttaaataaa
     4621 agcccggtca cattggactg accgggctta tgtgaattat tcgctgtatt cttcctgggt
     4681 aatctgtgtt tcttttggtg ggtttattcc ggcatcacga atattcttct gcaccgctac
     4741 tgcaccaaac aagcttaaga agaaagccag tccataaaaa cctttctcgc tgagtaataa
     4801 cgtcgcattc cacagaccta ccatcagtaa cgcaacagag ataataaaga cagtcaggca
     4861 ggtcatataa taaatggagg tggtcggtat gccttcatac ttgtcccgaa cggtcttttg
     4921 ataagacgcc gcagaaaaca gtcctaatac cagtacggca aaataatatc ctttttcatt
     4981 taactgcatc tctgcattcc atagccctaa cagataggta acgataccac caacgagagc
     5041 tatccatgac acaatactga atgccggaga ataggttgat attttgttgt ccatcacagt
     5101 attcctttca ttcctgaatg tgtaagaggt ttatgttgct catgccgagc gaaacaaacg
     5161 catttgacca aacaagcaga caaaaaaggc gatgggataa atcaccattt cataagcatt
     5221 gataggtgcg tttaaaacac cgataaacaa gagaccgata gtgatcaacg caactaactg
     5281 gcagatatgg ataaaaaaat catccagttg cccgagtttt tctgcgcgaa gatatgcata
     5341 gttaccaaaa gttgctgtca ttaacacgcc aagaaaatag ccttttccac ttaacaatgg
     5401 gcatgagatc catagcccaa ccagataaac cagcgcccca acaacaaata atagttttgc
     5461 aacagtcttt gaggttttca taacgatctc catatctacc agcgatacat tacgagtaac
     5521 caacgaaaga caaaactgaa aaatgccatt aacaaatgat ttcagaataa attca
//
LOCUS       DACBVI010000224         5555 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.226, whole genome shotgun sequence.
ACCESSION   DACBVI010000224 DACBVI010000000
VERSION     DACBVI010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.226"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            91..312
                     /locus_tag="IU325_21465"
     CDS             91..312
                     /locus_tag="IU325_21465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811562.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            743..1768
                     /locus_tag="IU325_21470"
     CDS             743..1768
                     /locus_tag="IU325_21470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP5811563.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKEANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1836..3017
                     /locus_tag="IU325_21475"
     CDS             1836..3017
                     /locus_tag="IU325_21475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP5811564.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFIVTVVIAIAISVGLFRFNKTYQIKTGQLRRTWP
                     VAVMLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            3027..4130
                     /locus_tag="IU325_21480"
     CDS             3027..4130
                     /locus_tag="IU325_21480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP5811565.1"
                     /translation="MTKVLLSHPPRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRTDCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFLGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            4138..4896
                     /locus_tag="IU325_21485"
     CDS             4138..4896
                     /locus_tag="IU325_21485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP5811566.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEDLALHYLERVRIAEHAQKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(5127..5242)
                     /gene="rrf"
                     /locus_tag="IU325_21490"
     rRNA            complement(5127..5242)
                     /gene="rrf"
                     /locus_tag="IU325_21490"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(5282..5357)
                     /locus_tag="IU325_21495"
     tRNA            complement(5282..5357)
                     /locus_tag="IU325_21495"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5322..5324),aa:Thr,seq:ggt)
     gene            complement(5372..5487)
                     /gene="rrf"
                     /locus_tag="IU325_21500"
     rRNA            complement(5372..5487)
                     /gene="rrf"
                     /locus_tag="IU325_21500"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1272 a         1322 c         1428 g         1533 t
ORIGIN      
        1 atttataacc tccggtgata aaatggcatt cagctcgtta ataagagagt taacttatta
       61 agcgttagcg tttattactg aggtaacacc atgaaaagat taattcctgt ggcattgctc
      121 accgcattgc tggcgggctg tgctcacgat tcgccctgcg ttccggttta tgacgatcag
      181 ggtcgactgg ttcacactaa tacctgtatg aaaggcacga ctcaggataa ctgggaaacg
      241 gctggggcta ttgctggcgg ggcagcagct gttgctggcc tgacgatggg gatcatcgct
      301 ctgtcgaaat aactgcacaa ggcgcggagt accgcgctct gtttaacgat acagtatctc
      361 tccataagat aaaatattgc tatattctgc tgggtaattc cctgctaatt tagcatgtta
      421 tatcgcgtta tttcgctgct ttcgaaataa cgcatcaatt taattttcac acttcctacc
      481 agaaatatac tcacgctaac aatcattatt ctgcttagta aaaaatgcgt aaatattttg
      541 cgccaaaatg tggcgcatgt ttcattttcg caccattgcg gggcgctgtt tttattattt
      601 cctgtctaca ctcagcttat tgcgacgcga aatcgtgcgc aaaagctggc actacttttg
      661 cttataagag gatggccaca gacaggtaaa agacgtttcc caaacgtcct ataacgataa
      721 ttttcgcctc acaggaagca ttatgaaaaa gatgatgata gccacactgg ctgccgccag
      781 cgtgctgctt gccgttgcaa atcaggcgca tgctggcgcg acgcttgatg ccgttcagaa
      841 aaaaggtttt gtgcaatgcg ggatcagtga tggattacct gggttctctt atgccgatgc
      901 tgacggtaag ttttcaggta ttgatgtaga tgtttgtcgt ggtgttgcgg cagctgtatt
      961 tggtgacgac acgaaagtga aatatacccc gctcactgca aaagaacgct tcacggcttt
     1021 acagtcaggg gaggtggatt tgctctcccg taatacgacc tggacttcat ctcgcgatgc
     1081 cgggatggga atggcattta ctggcgtcac ttattacgac ggcattggct tcctgactca
     1141 cgataaagcg ggattaaaaa gcgcgaaaga actggatggc gctaccgtct gtattcaggc
     1201 ggggactgat accgaactca acgttgccga ttacttcaag gcaaacaata tgaagtacac
     1261 accggtgact ttcgatcgct ctgacgaatc agcgaaggca ctggaatctg gtcgctgcga
     1321 tacgctggcc tcggatcaat cacaactgta tgccctgcgc atcaaattaa gcaacccagc
     1381 tgaatggatt gtcttaccgg aagttatctc aaaagaaccg cttggtccgg tagttcgtcg
     1441 tggcgatgat gaatggttct cgattgtacg ctggacgctt ttcgccatgc tgaatgctga
     1501 agagatgggc atcaattccc agaacgtcga tgaaaaagag gctaatccag caacgcctga
     1561 tatggcacat ctgttgggta aagaaggcga ttacggcaag gatctgaagt tggataataa
     1621 atgggcctat aacatcatca aacaggtggg taactactcg gaaatttttg agcgtaacgt
     1681 aggttcagaa agcccgctga aaattaaacg tgggcaaaat aatctctgga ataacggcgg
     1741 tattcagtac gcgccgcccg tgcgttaagt cggtgtgagg taacgagcgt cgctacggcg
     1801 gcgctctctt cagcgttatg ctttccgagg tttctatgtc tcatcgccgc tcaaccgtta
     1861 aaggctcact ctcctttgcc aaccctacgg ttcgtgcttg gttattccag atccttgccg
     1921 ttgttgctgt tgtcggcatt gttggttggt tatttcacaa cactgtaacg aatctcaata
     1981 atcgtggcat tacttcaggt tttgcctttc tggatcgcgg cgctggcttc ggtattgtcc
     2041 agcatttgat cgattaccag cagggcgaca cctacggacg cgtttttatt gtcggcttac
     2101 tcaatacgct actggtttct gcattgtgta ttgtgttcgc ttctgttttg ggcttcttta
     2161 tcggtctggc gagactttcg gataactggc tgctacgaaa gctttccaca atttatattg
     2221 agatcttccg taatattccg ccgctgctgc aaatcttctt ctggtacttt gccgtgttgc
     2281 gcaatttgcc cggaccacgc caggctgtga gcgcgtttga tctggccttt ttgagcaatc
     2341 gtgggcttta tattccgtca ccgcagctgg gagacggatt tattgcgttt atcgtgactg
     2401 ttgttattgc tatagccatt tctgttggac tattccgttt taataaaacg taccagataa
     2461 agaccggaca attgcgccga acctggccgg tcgccgttat gttgatcata ggtttgcctt
     2521 tactggcgca atggcttttt ggcgcagcac tacactggga tgttccagcc ctacgaggct
     2581 ttaacttccg tggcgggatg gttttaattc ctgaactggc agccttaacg ctggcgcttt
     2641 cggtttatac ctctgcattt atcgccgaga ttatccgcgc tgggatccag gcagtgcctt
     2701 atggtcaaca tgaagcggct cggtcactgg gattacccaa tccggttacg ctacgccagg
     2761 tcattattcc ccaggcactg cgagtgatta ttccgccgtt aaccagccag tatctcaaca
     2821 tcgtcaaaaa ctcctctctt gccgccgcga ttggctatcc ggatatggtt tcgctgtttg
     2881 ctggcaccgt gctgaaccag acggggcaag ccatcgagac gatagccatg accatgtcgg
     2941 tctatctgat tatcagcctg actatctcgc tgctgatgaa tatctataac cgccgcatcg
     3001 cgatcgttga acgctaagga gtcatgatga caaaagtatt gctgtctcat cccccgcgcc
     3061 cggcgagcca taactcaagc cgcgcgatgg tgtgggtgcg aaaaaatctg ttctccagct
     3121 ggagcaatag cctgctgact attggctgca tatggttgat gtgggaactg attccaccgt
     3181 tgctgaactg ggcatttttg caggctaact gggttggctc aacgcgtacc gactgcacaa
     3241 aagccggtgc ctgttgggtc ttcatccacg aacgatttgg tcagtttatg tatgggcttt
     3301 acccacacga ccaacgctgg cgaattaacc tcgcattact gattgggctt gtgtcgatcg
     3361 caccaatgtt ctggaaaata ctcccgcatc gcggtcgcta tattgcggta tgggcggtga
     3421 tttacccact gattgtctgg tggctgatgt atggcgggtt tcttggtctt gagcgggttg
     3481 aaacccggca atggggcggg ctgacgctaa ctttaattat tgcatcagtt gggattgcgg
     3541 gggcgctgcc gtgggggatc ttactggcgt taggtcgccg ctcccatatg ccgattgtgc
     3601 gtatcttatc ggtcattttt atcgagttct ggcgcggtgt accgctgatt accgttctgt
     3661 ttatgtcttc ggtcatgctg ccgttgttta tggcagaagg caccagtatc gacaaattga
     3721 tccgcgcgct ggttggcgtg atcctgtttc agtcagcata tgttgcggaa gtcgtgcgag
     3781 gcggattaca ggcgctgcct aaagggcaat atgaagcggc agagtcgctg gcgttgggtt
     3841 actggaaaac ccaggggctg gttattctgc cacaggcgtt gaagctggtg attcctgggc
     3901 tggtaaatac catcatcgca ctcttcaaag ataccagcct ggtgatcatc atcgggttgt
     3961 tcgatctttt cagtagcgtt cagcaggcaa ccgttgatcc cgcctggttg ggtatgtcga
     4021 cggaagggta tgttttcgcc gcactgatct actggatctt ctgtttcagc atgtcacgtt
     4081 atagccaaca tctggaaaaa cgttttaaca ccgggcgtac accgcattga ggacactatg
     4141 agccaaattt tactgcaacc tgctaacgcg atgattacgc tggaaaacgt caataaatgg
     4201 tatgggcaat tccatgtttt gaaaaatata aatttaaccg tgcaaccggg agaacggatc
     4261 gttctgtgtg gcccttctgg ttccggtaaa tcgacaacca ttcgttgtat taatcatctg
     4321 gaagaacatc aacagggacg gatcgtggtg gatggcatcg aacttaatga agatatccgc
     4381 aatattgagc gcgtcaggca ggaagtggga atggtctttc agcatttcaa tctcttccct
     4441 catctgaccg ttctacagaa ctgtaccctg gcaccgattt gggtacgcaa gatgcctaag
     4501 aaagaggctg aagatctggc gttgcattac cttgagcggg tgagaattgc cgaacatgcc
     4561 cagaagtttc ccggacagat ttctggtggt cagcagcaac gcgttgccat agcacgttca
     4621 ctttgtatga aaccaaagat catgctattt gatgaaccta cgtcagcgct cgatccggag
     4681 atggtgaaag aagtgctgga tacgatgatt gggctggcgc agtcgggtat gacaatgttg
     4741 tgtgtaacac atgagatggg gtttgcacga accgtcgctg acagggtgat ttttatggat
     4801 cgtggggaaa tagtggagca agctgcacct gatgaatttt ttgcgcatcc taaatcagag
     4861 cgtacgaggg catttttatc gcaggtaatc cattaattga atgttagttc gaaaagcaaa
     4921 aaggccatcc tttcggatgg cctttcgctt gatttgatgt ctggcagttt atggcgggcg
     4981 tcctgcccgc caccctccgg gccgttgctt cgcaacgttc aaatccgctc ccggcggatt
     5041 tgtcctactc gggagggtgt tcaccgacaa acagcagata aaacaaaagg cccagtcttc
     5101 cgactgagcc ttttgtttta tttgatgtct ggcagttccc tactctcgca tggggagacc
     5161 ccacactacc atcggcgcta cggcgtttca cttctgagtt cggcatgggg tcaggtggga
     5221 ccaccgcgct actgccgcca gacaaattct tttctaatct gccgaacttt aacctaaaaa
     5281 gtggtgctga tacccagagt cgaactgggg acctcaccct taccaagggt gcgctctacc
     5341 aactgagcca tatcagcacg ctaaatttga tgcctggcag ttccctactc tcgcatgggg
     5401 agaccccaca ctaccatcgg cgctacggcg tttcacttct gagttcggca tgggatcagg
     5461 tgggaccacc gcgctaaggc cgccaggcaa attctgtttc atcagaccgc ttctgcgttc
     5521 tgatttaatc tgtatcaggc tgaaaatctt ctctc
//
LOCUS       DACBVI010000225         5537 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.227, whole genome shotgun sequence.
ACCESSION   DACBVI010000225 DACBVI010000000
VERSION     DACBVI010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.227"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            1070..1786
                     /gene="nanC"
                     /locus_tag="IU325_21505"
     CDS             1070..1786
                     /gene="nanC"
                     /locus_tag="IU325_21505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HAP5811567.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1806..2912
                     /locus_tag="IU325_21510"
     CDS             1806..2912
                     /locus_tag="IU325_21510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjhT family mutarotase"
                     /protein_id="HAP5811568.1"
                     /translation="MNKTITALAIMMASFATNASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVSK
                     GDKTWLINGEAKPGLRTDAVFEIDFTGNNLKWNKLAPVASPDGVAGGFAGMSNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSADIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2977..3957
                     /locus_tag="IU325_21515"
     CDS             2977..3957
                     /locus_tag="IU325_21515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HAP5811569.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGSDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILRFWRER"
     gene            4080..4761
                     /locus_tag="IU325_21520"
                     /pseudo
     CDS             4080..4761
                     /locus_tag="IU325_21520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4931..>5537
                     /locus_tag="IU325_21525"
     CDS             4931..>5537
                     /locus_tag="IU325_21525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811570.1"
                     /translation="MNIRQSLHDRIYAEYPQQAKEILLYFPTYEIMPSTIKMVMINEV
                     VAITPQEDFYTPESGYFTTVARQLALAGLSASSGEVLLNHGIYLTSALKLPKTQITAT
                     PAEIILWSPIVEWELELFANIKVIFVGGDIARKCLNIIAKKQTRKHAIPSTPTYKLMN
                     SELVWKGISILPGYILTGKNLLIEKSKIEMNVEQLRKGIAIY"
BASE COUNT         1628 a         1220 c         1242 g         1447 t
ORIGIN      
        1 ctgcatttgc cacaaaatgg ctatttcatg gaacgcagta atttcttagt ggctattatc
       61 aagctaattc ttaggtccct agcgattatt cctggctggt tcgtcagttt aggtcatgag
      121 attgttacat tcctttttat cctggagata tgggggaacg caggcgacgc tttttcatct
      181 acatccgttg gtaaatatgg ctttggtcac tacgacttaa accctcctca atcacagcta
      241 ctcgccgggc gatacggcaa acaaactaat tgaaacatca tcctgaaaac accagcatca
      301 acaagcctct ccagatcgac atcaggagtg accagttaca agccacaaac caggaaacat
      361 atttcatgat gagaattatg ctcagcctga tggagggtaa acacttattt tcaccaagct
      421 cactgagtta gcttgcaaag ctttctttgt ttagattcta aataaattat ttataaaaca
      481 gatagttaaa agaaaatctt tagtttttga agaccaccgc aagtgttcgt ctggcttcac
      541 atagcatctg cctcttagaa aaagatcgac atattttgtg acacgaattg caaatctggt
      601 tttgttgtat ggattgcgtg atttttgatc tggtataaca ggtataaagg tgcaccacga
      661 tagtcaatga gactgggcat ctcgtaatct atggcaaaca tcacttcagt tctttcacat
      721 cgggtgatga aaatgcactt cagtctgaaa ggaatatgaa aatgagatca acagacactc
      781 tattttatga ctctgggtaa aatggattga gtaagtgata tagcttacga acattcaaat
      841 caattaaaca tcagaagaga ttttatactc aggtatttaa tctggttctc tgtttattta
      901 aataatgtgg aaagagaatt ttcacaggag accttataca aaaaatataa aataagctac
      961 ccgttgccaa agacactata agcctggcaa aaaatattac acaacataaa tgctaattgt
     1021 ttatgcgggc tttgtattgc tttctgtatc ctacaaatga gtgaaattta tgaaaaaggc
     1081 taaaatactt tctggcgtat tattactgtg cttttcgtca ccattaattt ctcaagctgc
     1141 gacactggac gtacgtggtg gatatcgtag cggaagccat gcctatgaga ctcgactcaa
     1201 agtcagtgag ggatggcaaa atggatggtg ggcaagcatg gaaagtaata cctggaatac
     1261 cattcatgat aataaaaagg aaaatgccgc actcaatgat gttcaggttg aagttaatta
     1321 cgcgattaaa cttgatgatc aatggacggt gcgcccggga atgttaacgc attttagcag
     1381 caacggcaca cgctacggac cctacgtaaa actgtcctgg gacgcgacaa aagatcttaa
     1441 ttttggcatt cgctatcgtt acgactggaa agcttaccga caacaagact tatccggtga
     1501 tatgtctcgt gataacgttc atcgttggga tggatatgtc acttaccata ttaatagtga
     1561 tttcaccttc gcatggcaaa cgacgctata cagcaaacag aacgattatc gctatgcaaa
     1621 ccataagaaa tgggcgacgg aaaatgcatt tgttctacaa taccatatga cgcccgatat
     1681 tacgccatac atagaatatg actaccttga ccgtcagggt gtttacaacg gcagagataa
     1741 tttatcggaa aacagttatc gcattggtgt gtcatttaaa ctgtagtaga caggagacag
     1801 tcacaatgaa taaaacaata acggcgcttg ctatcatgat ggcttcattt gccacaaacg
     1861 cgtctgtatt accggaaact cctgtgccat ttaaaagtgg taccggagca attgataacg
     1921 acactgtcta cattggttta ggtagcgcag gtacagcatg gtacaagcta gatacacagg
     1981 ccaaagataa aaaatggaca gcgttagctg cattcccagg tggaccgaga gatcaagcaa
     2041 cctctgcatt tattgatggc aatctgtatg tgtttggcgg cattggcaaa aacagcgagg
     2101 gattgactca ggtatttaat gacgtacaca aatacaaccc caaaaccaat agctgggtta
     2161 aattgatgtc gcacgcgccg atgggcatgg cgggccatgt gacttttgta cacaacggca
     2221 aagcttatgt tactggcggt gttaaccaga atatcttcaa tggctatttt gaagatctca
     2281 acgaggcagg aaaagattca acagctatag ataaaatcaa cgcccactat tttgacaaaa
     2341 aagcagaaga ttatttcttc aataagtttc tgttgtcttt tgatccctca acacagcaat
     2401 ggagttacgc tggcgaatct ccctggtacg ggacggctgg tgcggcggtt gtgagtaaag
     2461 gtgataaaac ctggcttatt aatggcgaag ccaaaccagg attgcgaacg gatgccgtat
     2521 ttgaaattga tttcaccggt aataatttaa aatggaataa gcttgctccc gtcgcatcac
     2581 cagatggcgt cgctggcggt tttgcaggca tgagcaatga ctcactgata tttgccggag
     2641 gggccggatt caaaggttca cgggaaaatt accagaacgg taagaactat gcgcatgaag
     2701 gcctgaaaaa atcatatagc gctgatattc atctttggca taacgggaaa tgggataaat
     2761 ctggtgaatt atcgcaaggt cgggcctacg gagtatcatt gccctggaat aatagtttat
     2821 taattattgg cggtgaaact gcaggcggca aagcggtgac ggattcagtt ttaatttctg
     2881 tgaaggataa taaagtcaca gtacaaaact aacgcttcag ggccccggta aaggggccct
     2941 gctatcaatt tgtcatttgt attacgcgaa aattatatga acgcaataat atcgcccgat
     3001 tattactatg ttcttaccgt tgctggtcag tctaatgcca tggcgtatgg cgaaggactg
     3061 ccattaccgg acagggaaga tgcgcctcat cccagaatta aacaattagc gagatttgcg
     3121 catacgcatc ccggaggccc gtcatgtcac tttaacgaca ttattccact gactcactgc
     3181 ccacacgatg ttcaggatat gcagggttat caccatcctc tggcaacgaa tcatcaaaca
     3241 cagtacggca ccgttggcca ggcactgcat attgcacgga aattactgcc ctttattcct
     3301 gataacgcag gggttctcat cgttccgtgt tgccgtggcg gatcggcttt taccgcgggc
     3361 agcgaaggga catattcaga acggcacgga gccagccatg atgcttgtcg ttggggatcg
     3421 gatactccgc tataccagga tttagtcagc agaacgcgag ccgcactggc aaaaaatccg
     3481 cagaacaaat tcctcggcgt atgctggatg caaggcgaat ttgacttaat gaccagtgac
     3541 tacgcgtcac accctcaaca ctttaatcat atggttgaag cctttcgtag ggatctaaaa
     3601 caataccatt ctcagcttaa taatattact gacgcaccgt ggttttgcgg cgataccacc
     3661 tggtactgga aagaaaattt ccctcatgcg tatgaagcga tttatggcaa ttatcaaaat
     3721 aatgttttag ccaatattat tttcgtcgac ttccagcaac aaggtgaaag aggactgacg
     3781 aacgcgcctg atgaagatcc ggacgattta agcacgggat attacggttc agcgtaccgg
     3841 tcaccggaga actggacgac ggcactgcga agcagtcatt tcagcgcggc agcccgtcgg
     3901 gggattattt ctgacaggtt tgtagaagca attttgcggt tttggcgcga aaggtgagcg
     3961 ttcattaatt tgatttattt gcatccatta gcgcttttaa ctgctccggc gtgcggcact
     4021 ataatggttt tcacaacaag gagccatttt cttataccac tttcagagac catccgctta
     4081 tgccaccgcc ctctacgcaa ctgtttgcag agcaccttcc caccgagtgg atacagcacc
     4141 tcctgacgct ttctgctcat gcgaccattt gtcgtcttaa cctgagcgcg gatggcgaag
     4201 cggggattag tctgctggcc cgcagcgctg tcacccagaa gcgccagcgc gtgggggccc
     4261 cctggtggaa tggctcttcc gccagaccgc acagacatgg ggcgcggaac gttacctgaa
     4321 ggatgactgg cacggcctgc aactttttgc cattgatggc gcacagttca ggacacctat
     4381 gaacctgagt tgcgcgaata ttatggctct gccaacacat ccactgagcg gcagagcgcc
     4441 tacccggtca tgcgtctggt ggcgttaatg aatctgggaa tcacattctg ctgaatgcca
     4501 tgaccgcggc ctaccggcgg agcgatacag cgtgaggcaa ctaaagcggc gtcagaaatc
     4561 agctttaagt tcgcattcca gtttatcgtg acagaaatga tagtgcaggg aaatacggcg
     4621 tcaccaggaa ccatcccgaa acggctggag catctgcggg gtgctctgga agcggtgttc
     4681 ccacgactat caaggccgag ggctgttaag gtatcaaaaa cccgttatcc ggtgaaacac
     4741 agtgccgccc cgcttaagtg acggcattgt gtttctgact ggccttaact ttccctgaat
     4801 ctctgacgat cctcaccatt gatattttct tcattcctca ccagagaaat aaaaaatcac
     4861 tatactcatc aaaatatgag attaccttta ttcaggcgat caccttacta ttttatacgg
     4921 aaaataaatc atgaatatcc gtcaatcatt acatgaccga atctatgcag aataccctca
     4981 acaggctaaa gaaatattac tgtatttccc cacttatgaa attatgccat cgaccataaa
     5041 aatggttatg attaatgagg tggtagctat aacaccgcaa gaagattttt atacaccaga
     5101 gtctggttat tttacgactg tcgcccgaca gttagcgctg gctggattat cggcaagtag
     5161 tggcgaagtc ttactcaacc atggtatcta cctgacctcc gcactgaaac tacctaaaac
     5221 acagatcaca gccacacccg cggagatcat tctctggtca cctattgtag aatgggagct
     5281 cgaactcttt gccaatataa aagttatttt cgttggcggc gatatcgcgc gaaaatgcct
     5341 taacatcatc gcgaaaaagc aaacacgtaa acatgccatc ccttcaactc cgacgtacaa
     5401 actgatgaat agcgaattag tctggaaagg aataagcata cttcctggat atattcttac
     5461 cggaaaaaac ctgctcattg aaaagtcaaa aatcgaaatg aatgtcgaac aattaagaaa
     5521 aggaattgcg atttatc
//
LOCUS       DACBVI010000226         5533 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.228, whole genome shotgun sequence.
ACCESSION   DACBVI010000226 DACBVI010000000
VERSION     DACBVI010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.228"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(33..752)
                     /gene="dcuR"
                     /locus_tag="IU325_21530"
     CDS             complement(33..752)
                     /gene="dcuR"
                     /locus_tag="IU325_21530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HAP5811571.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(749..2380)
                     /gene="dcuS"
                     /locus_tag="IU325_21535"
     CDS             complement(749..2380)
                     /gene="dcuS"
                     /locus_tag="IU325_21535"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HAP5811572.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2560..2790
                     /gene="yjdI"
                     /locus_tag="IU325_21540"
     CDS             2560..2790
                     /gene="yjdI"
                     /locus_tag="IU325_21540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HAP5811573.1"
                     /translation="MDQDLLDGGYRCYTGEKIDVWFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVTTVVKVIDTCPSGALKYRHK"
     gene            2802..3074
                     /locus_tag="IU325_21545"
     CDS             2802..3074
                     /locus_tag="IU325_21545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP5811574.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            3303..3599
                     /gene="ghoS"
                     /locus_tag="IU325_21550"
     CDS             3303..3599
                     /gene="ghoS"
                     /locus_tag="IU325_21550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HAP5811575.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            3627..3800
                     /gene="ghoT"
                     /locus_tag="IU325_21555"
     CDS             3627..3800
                     /gene="ghoT"
                     /locus_tag="IU325_21555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HAP5811576.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(3919..5436)
                     /gene="lysS"
                     /locus_tag="IU325_21560"
     CDS             complement(3919..5436)
                     /gene="lysS"
                     /locus_tag="IU325_21560"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP5811577.1"
                     /translation="MSERETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERIFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
BASE COUNT         1442 a         1283 c         1308 g         1500 t
ORIGIN      
        1 ataattatta cttacagata accagcgatc cgttattggc aatattgttt cagcagtgag
       61 tagtgttctg cctgaatacg gtaacggtaa accggacgcc ccgtgacgcc ataatggata
      121 ctggtgaaca agatgtggca gttgaccagc cagatgaggt atttacggca ggaaacacgc
      181 gaaatgttaa cctcgttagc cagctcgtcg gttgaaaact catagtcctg atgcgcgtca
      241 atccactggc acagcgtgcg taacgtctgc ggcgttaagc cttttggcaa gcgacgagga
      301 tcctgctcgt tggagctgct gccgtggatt agctgatcaa gctcggcctg gtcataatac
      361 tgatgttttt ccagcgccat tttcttttgc cgccagccgg tgagcgcctc ttcaaagcgg
      421 gaagcctgga agggtttgat caggtaatcc acgacaccgt aatgcagcga atctttaatg
      481 gttgccgcat cggctgcgga ggagatgaca atcacatcac ttttgcaacg cgcgttatgc
      541 aggacaggca gtaaatcgag cccgttctct ttttgcatat agatatcgag caatatcagg
      601 tcgataggcg tatcgctatt gaagataatc tctttggctt tctccagcgt cgaggctgtt
      661 ccacagcatt gaaagcctgg gatttgtgct acgtatcggc gattcagctc cgcgaccatt
      721 gcgtcgtcat cgataattaa tacattgatc atctgttcga cctctccccg tcccagggta
      781 tctggacaaa aaattgtgtg aaaatcccgg gttccgattc cacggcgatg ctgccgccga
      841 gattttctac ctgttgtttg acaagtgcta aaccgacgcc tcgctcgctt ccttttgtcg
      901 agacaccttt gtcaaaaatg tgatcgattt tatcgggtgc gatccccggt ccatcatcat
      961 taacttcaca gtgcagccag ccgtgacggt agtgcaatgt tacgctaatt tcgcctccgg
     1021 gttccggccc taatgcttcc agcgcgtttt ctatcagatt tcccaacgtg gtaatcagcg
     1081 tcgcgacctg gtcttcactg ccgctgtctg gcagctggct ttcactgttt aaaatcagcg
     1141 tatggcctaa atcggtcgcg cggttaatct tgctgattaa aaaaccagct ataaccggag
     1201 atttgatctt acccagcaga gagccaatct cttcctgata gttattggct gttttgagaa
     1261 tgtaatcttc caactgctta taactcttca gatgcaataa tccgagaatc acgtgcaatt
     1321 tattcataaa ttcgtgggat cgttcacgaa gtgcgtcagc atagttgacc agaccgtcga
     1381 gtcgctgcat cagtttacgt acttcagttt tgtctctgaa ggttgaaatg gcaccgatga
     1441 taacgccatt actgcgcacc ggaacggtgt tgatcagtaa tagccggtct ttaatcgtaa
     1501 tctcttcgtc gcggcgcggg gtaccgtcgc gtaacacttc cgagacatct actacctgtg
     1561 accacgagtg gcttagcgtc gacagtttct catcgtcctg cgacttacgg taattcagca
     1621 attcttgtgc ggcatcgttg atcagcgtga cttcgccgcg atcgtccacg gcaacgacgc
     1681 cttctttgat agactgcaac atggcctggc gttgctcaaa cagcgtggag atttcgtagg
     1741 gttccaggcc aaaaagaatt tttttcagta ctttaaccag aatgcaggtg ccgatcagtc
     1801 cgaccagcat gccaaataat accgaccaga taatgctcca gcgactgtca ttgatctgtt
     1861 gggtcacgcg acttaactca aggccgatcg ccaccacgcc aatttgttta tgattttcat
     1921 cgtagatggg ggtaaatacg cgtaaagcct gcgccagaaa accgcgattg atagcgacat
     1981 tttcttcgcc attcagcgct ttaaggatgt catcaccttt aaatggctga ccaatacgct
     2041 gggcttcagg atgcgagtag cgaagacttt gcatatcggt aacgacaata aacagcagat
     2101 cgttgcgttt gcgcacggct tccgcgatgg cctggatgcc gctctcctgc ggttttttct
     2161 gcaagccctg acggatttcc ggcgagtcgg cgagggtacg cgccaccgcc agtgccttgt
     2221 tggctagccc gtctcgcgtc atatcactga tttgcgagaa gtaaatcaga tgcaccacca
     2281 atagcaccga gaacagtacc gcgctgacca ttaagatcac tgtggtactc aatttcatcg
     2341 gacgtttgcg taacatgcgg tagggcaatg aatgtctcat cagcttcctt gtgtgacaaa
     2401 tttcttaagc attatctctg atgaggcggg taattcaaag ggagtaagaa tgattggcta
     2461 tataggggaa gaggctcagg aaactgaaac tgccggtgcg gtatgaagat tccgggctat
     2521 gcttattgcg aaaaccatac tgatgagagg gggaaggtca tggatcagga tctactggac
     2581 ggtggttatc gctgctatac cggcgaaaag atcgatgtct ggttcaacac tgcgatatgt
     2641 cagcattctg gcaattgcgt acgtggcaac ggcaagttat ttaatctcaa acgaaagccg
     2701 tggatcatgc cggatgaagt tgacgtcacc actgtggtta aagtgattga tacgtgcccg
     2761 agcggcgcgc tgaaataccg tcataaataa gcgagggtaa aatggaaata cgcgaaggcc
     2821 acaataaatt ttacattaat gacgaacaag gcaagcaaat cgctgaaatt gtctttgtgc
     2881 cgaccggaga gaatttagcg attatcgaac ataccgatgt cgatgaaagc ctgaaagggc
     2941 aggggattgg taaacagctg gttgcgaaag tcgtggaaaa aatgcgtcgg gaaaaacgaa
     3001 aaattatccc actatgccca tttgcgaaac acgaatttga taaaacgcgg gagtatgatg
     3061 atattcgcag ttgatgggag agtacagagt cacgatattt ttcatgctct ccgcggtgtg
     3121 atgcaggaga gcatctgaag ggtaggggga tgcacaaaga atgggcagag agagcgtttt
     3181 tttgtcccaa gtcatcccct ttactgagca aaaaaaaaag aatatctcct atatgagaat
     3241 catcgattgg ggttaataag ttttgcgtcc ccagagcgtt taaaattgat aggagtcata
     3301 ttatggaagg taaaaacaag ttcaatactt atgttgtttc ttttgattat ccatcatctt
     3361 attcctcagt gttcttaagg ttaagatcat tgatgtatga tatgaatttc tcctctatcg
     3421 tggctgatga atatgggata ccacgacaat tgaatgaaaa ttccttcgca ataacgacat
     3481 cattagccgc aagtgaaatc gaagatttaa tcaggctcaa atgcttagac ttaccggata
     3541 ttgattttga cctcaacatt atgacagttg atgactattt ccgtcagttt tacaagtagc
     3601 caggaaggat aagaagaaca taaaaaatgg cactattctc taaaatatta attttttatg
     3661 tgattggtgt gaacatatcc tttgtcatta tctggtttat ctcacatgag aaaacacata
     3721 ttcgtttact tagtgcattc ctggtcggaa taacctggcc aatgagtctg cctgtggcat
     3781 tacttttttc tctcttttag gagctgtagt tgagtggacc cgctggttat cttgaaacga
     3841 agcgtctctc ctcaaaactg gatttgcgga acgacgaacg aagcagtcgc ctgactgctt
     3901 cgttcattaa agtgaaattt atttctgtgg gcgcatcgcc gggaagagaa taacgtcgcg
     3961 aatagtatgg ctgtttgtaa acagcataat cattcggtcg ataccaatac ccagaccagc
     4021 ggttggcggc agaccatatt ccagcgcagt cacgtaatct tcgtcataga acatggcttc
     4081 gtcgtcacct gcagctttag cgttaacctg ttcctggaaa cgttcagcct gatcttctgc
     4141 gtcgtttaat tcgctaaaac cattaccgat ttcacgacca ccgatgaaga attcaaaacg
     4201 gtcggtgatt tccgggttaa catcattacg gcgcgccagc ggggacactt ctgccggata
     4261 ttccgtaata aaggttggct gaatcagatg tgcttctgcc acttcatcaa agatctccgt
     4321 cacaatacgt cccagccccc agcttttctc taccgtaatt ccgatagatt cagctaatgc
     4381 tttagcggca tcaaaattat ccaggtcggc catatcggtt tctggacgat attttttgat
     4441 tgcttcgcgc atggtgagtt tttcaaacgg tttgccgaaa tcaaacacat gctcgccata
     4501 agtgacttta gtggtaccca gaacttcttg tgccagggtg cggaacaggg actccgtcag
     4561 ttcaatcaaa tcgtggtaat ccgcatacgc catgtagagt tccatcattg tgaactcagg
     4621 attatggcga acagaaatac cttcattacg gaagttacgg ttgatttcga atatccgttc
     4681 aaaaccgcct acaaccagac gtttcagata cagctccggc gcgatacgca ggtacatatc
     4741 taaatccaga gcgttatgat gggtaataaa cgggcgagca gatgccccac ctggaattac
     4801 ctgcatcatc ggggtttcta cttccataaa gccgcgcgcg accatgaact gacggatagc
     4861 ggccagaatt tttgaacgga caacaaacgt ttgacgggat ttatcgttag cgatgaggtc
     4921 cagataacgt tgacgataac ggacttcctg atcctgcaga ccatggaatt tatctggtaa
     4981 aggacgtagt gctttagtca gcaggcgcag ctcagtacag tgaatggaaa gctcaccggt
     5041 ttgcgtctta aacagcgtac cgcgggcacc gataatgtca cccagatccc attttttaaa
     5101 ctgatcgtta taaacacctt ctggcaggct atctcttgca acgtacagtt gaatacggcc
     5161 accgacatcc tgcaacgtta caaaggaggc tttccccatg atacgacggg tcatcattcg
     5221 gccagcaacc gagacttcaa tgtttaagga ttccagttcc tggttatcct tcgcatcaaa
     5281 ctcttcgtgc aactggtcag aggtatggtc gcggcgaaaa tcattaggaa acgccacacc
     5341 ttgctgacgc agtgccgcca gtttttcgcg gcgatttctc agttcatcgt taaaatcaat
     5401 agcctcattg gctccccgtg tttctcgttc agacattttg gttcctctaa atccagcttt
     5461 caatttagcg tagataaaga gacagatcgg tacactgtat tatctgcctc gactattaat
     5521 aactcagttt atc
//
LOCUS       DACBVI010000227         5477 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.229, whole genome shotgun sequence.
ACCESSION   DACBVI010000227 DACBVI010000000
VERSION     DACBVI010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.229"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            125..835
                     /locus_tag="IU325_21565"
     CDS             125..835
                     /locus_tag="IU325_21565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAP5811578.1"
                     /translation="MQTRRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENEGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            877..3528
                     /locus_tag="IU325_21570"
     CDS             877..3528
                     /locus_tag="IU325_21570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAP5811579.1"
                     /translation="MTAFRAAFKAYRMHQVLLLPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHLAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSIKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDSNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSATSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVIHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPEVSPGTLLNQQTAICR"
     gene            3542..4072
                     /locus_tag="IU325_21575"
     CDS             3542..4072
                     /locus_tag="IU325_21575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP5811580.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKETTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLIPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            4085..4588
                     /locus_tag="IU325_21580"
     CDS             4085..4588
                     /locus_tag="IU325_21580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAP5811581.1"
                     /translation="MKRLHKRFLLATFCALFTVTLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            4648..>5477
                     /locus_tag="IU325_21585"
     CDS             4648..>5477
                     /locus_tag="IU325_21585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP5811582.1"
                     /translation="MGKTISMKLLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVNVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     "
BASE COUNT         1571 a         1196 c         1222 g         1488 t
ORIGIN      
        1 acatttatta caaacgcatt aagtctggaa acccggcgag tcgccaatgt attagcaaca
       61 tttaccgatt gtctgatgta acggtttccc cgcttatcct cttaacacat ggagaatgca
      121 tcagatgcaa actaggcgta ccccctactc tatttccttt atggcgacag tgttactttt
      181 attgctcttt gcttgccata gcaccgttgc caacgcagct gtcgcactgg gtgcgacgcg
      241 ggtaatttat ccggcaaacc agaaacaggt tttattgcct gtcacgaata atgatcctgc
      301 cagtgtctat ctcattcagt catggataga aaatgagggt gaccaaaaag acacccagtt
      361 tgtcattacg ccaccgcttt tttccatgca aggtaaaaaa gagaatacgc tgcggataat
      421 caacgccact aatcatcagt taccaggcga ccgtgaaagc ctgttctggg ttaatgttaa
      481 agctattcct gctatggaaa aagatcagaa gaatgagaat acattacagc tggcaattat
      541 tagccgcata aaaatgtttt atcgccccac gaacctggca atggcacctg aagaagcgcc
      601 tgcaatgctt cggtttcgtc gcagcggcag caaactcact cttattaatc caacgcccta
      661 cttcattacc gtaacaaata tgaaagcagg aaatagcaat ctcccaaaca ccatggtgcc
      721 gccaaaaggt gaagtatcgg tggatatccc gcatgctgca accggggata tcagttttca
      781 gacgatcaat gattacggtg cattaactcc gcgtattaag gcaaccatgc aataagccag
      841 tgacctaatt aataataaac tcaatcggaa ttaaaaatga cagcctttcg tgccgcattt
      901 aaggcatatc gtatgcatca ggtgttacta ctgcctcgct ttgcacggct taccattgcc
      961 cttggcttag caactgcggt atttcccgtt gacgcagagt attattttaa tccacggttt
     1021 ttaagcaatg atcttgctga atctgtcgat ttatccgcct ttactaaggg acgcgaggca
     1081 ccacctggta catatcgggt cgacatttat ttgaacgacg aatttatgac tagtcgggat
     1141 attactttta ttgcagatga taataacgca gatctcatcc catgcctgag tacagatctt
     1201 cttgtcagtc ttggcataaa aaaatcagcg ttattagaca ataaagaaca tttggcagaa
     1261 aaacatgtgc cggacaacag cgcatgcaca ccacttcagg atcgtttggc cgacgcatca
     1321 actgagtttg atgttggtca gcaacatttg tcactctccg ttccgcaaat ttatgttggc
     1381 agaatggctc gcggctatgt ttctcctgat ctgtgggaag aaggaataaa tgctgggctt
     1441 ctaaactata gttttaatgg taattctatt aataatcgta gtaaccataa tgcaggaaaa
     1501 tccaactatg catatttgaa tttacagagt ggcatcaaca ttggtagttg gcgactgcgc
     1561 gataactcaa cgtggagtta taacagtggg agtagcaatt catctgacag caataaatgg
     1621 cagcacatca atacgtcggc tgaacgtgac attattccct tacgctcacg cttaacggta
     1681 ggtgatagtt ataccgatgg cgatattttt gatagtgtga actttcgtgg cctcaaaata
     1741 aattcaacag aagcgatgtt gcccgatagc caacatggtt tcgctccggt gattcatggt
     1801 attgcccgcg gcaccgcaca agtgagtgta aaacagaatg gatacgatgt ttatcagact
     1861 actgtcccac ccggcccttt tactattgat gatatcaact ctgcggccaa tggtggtgat
     1921 ctgcaagtaa ccataaaaga ggcagacggc agtattcaga cattatatgt tccttattcg
     1981 tctgttccgg ttctccaacg tgctggatat acgcgttatg cgcttgccat gggggaatat
     2041 cgtagtggaa ataacctgca aagctccccc aagttcatac aaggtagctt gatgcatgga
     2101 ctggaaggaa actggacacc ttatggcgga atgcaaattg cagaagatta tcaagccttc
     2161 aaccttggta ttggtaaaga tttaggactt tttggtgcct tttctttcga tatcacgcag
     2221 gccaatacga cacttgcaga tggcacccgt cacagcgggc aatcgattaa atccgtctac
     2281 agcaaatcct tctaccagac gggaaccaat atccaggtcg caggatatcg ctattctacg
     2341 caaggttttt ataacttatc cgacagtgcc tacagtcgaa tgagtggtta caccgtcaag
     2401 cctcctaccg gagacagcaa tgagcagaca caatttattg attattttaa tctgttctac
     2461 agtaagcgtg gtcaggaaca aataagcatc tctcagcagc ttggaaatta cggtacgaca
     2521 tttttcagtg ccagtcgcca aagttactgg aacacgtcac gcagcgacca gcaaatatca
     2581 tttggattaa atgtgccgtt tggtgatatt acgacttcgc tgaattacag ctattccaat
     2641 aatatatggc aaaacgatcg ggatcattta cttgctttta cgcttaatgt tcccttcagt
     2701 cattggatgc gtacagacag tcagtcggca tttcgtaatt caaacgccag ttacagtatg
     2761 tcaaacgatt tgaaaggcgg catgaccaat ctatcggggg tttatggcac tctgctgccg
     2821 gataataacc tgaattatag cgttcaggtc ggtaacaccc acgggggtaa tacatcgtct
     2881 gccaccagtg gttacagttc tcttaattat cgtggagctt acggtaatac taatgtcggt
     2941 tacagtcgga gtggtgacag cagccagatt tattacggaa tgagtggtgg gattattgct
     3001 catgctgatg gcatcacctt tggtcagccg ctgggcgaca caatggttct ggttaaggct
     3061 cctggcgctg ataatgtcaa aatagagaac cagaccggaa ttcataccga ctggcgtggc
     3121 tatgccatat taccatttgc gacagaatat agagaaaatc gtgtcgctct taacgcgaat
     3181 tcccttgcag ataatgttga actggatgaa accgttgtca ctgtcatccc aactcacggt
     3241 gctattgcca gagcaacatt taatgcacaa atcggcggga aagtattaat gacgttgaag
     3301 tacggtaata agagcgttcc attcggtgca attgtcatac atggtgagaa taaaaatggc
     3361 agcattgtcg cggaaaacgg tcaggtttat ctgactggac ttccacagtc agggaaatta
     3421 caggtttcat ggggcaaaga taaaaactca aactgtattg tcgagtacaa gcttcctgaa
     3481 gtttctcctg gtaccttact gaaccagcag acagcaatct gtcgctaagg aacaatagat
     3541 tatgaaatac aataacatta ttttcctcgg tttatgtctg gggttaacca cctattctgc
     3601 tttatccgca gatagcgtta ttaaaattag cgggcgcgtc ctcgattatg gctgcacagt
     3661 ctcatcggat tcgcttaatt ttaccgtaga tctccaaaaa aacagtgcca gacaatttcc
     3721 aacgaccggt agcacaagtc cagccgtccc ttttcagatt acgttaagtg aatgcagtaa
     3781 agagacaacg ggggttcggg ttgcatttaa cggtattgag gatgcagaaa ataatactct
     3841 gttgaaactg gatgaaggaa gcaatacggc ctccggtttg ggtatagaaa tactggacgg
     3901 aaatatgcgt ccggtgaaat tgaatgacct tcatgccggg atgcagtgga tcccactgat
     3961 accagaacag aacaatattt tgccttactc cgctcgtctg aagtcaactc agaagtccgt
     4021 caatccggga ctggtgaggg cttcggcaac ctttaccctt gaatttcaat aatcagagtc
     4081 agatatgaaa agacttcaca agaggttcct gttagctacg ttttgcgcgt tattcacagt
     4141 aactctccag gccgccgatg tcactatcac tgttaatggt cgggtagtcg ctaaaccctg
     4201 cactattcaa accaaagaag ctaacgttaa tctcggggat ctttatacgc gcaatctgca
     4261 acaacctggt tctgcatcag gctggcacaa tattactttg tcattaaccg attgtccggt
     4321 tgaaacaagt gcagtgacgg caatcgtgac aggttcaact gacaatacgg gttattacaa
     4381 aaatgaaggt actgccgaaa atattcagat agagctaagg gatgaccagg atgcgacgtt
     4441 aaaaaatggc gatagcaaaa cggttattgt tgatgagatc actcgtaatg cacagtttcc
     4501 acttaaggca agagctatca cggtgaatgg aaacgcaagc cagggaacga tcgaggcgct
     4561 aatcaatgtg atctacacct ggcaataacg tcgattcgat tttcttagcc tcctttttaa
     4621 atcaattttt taaccttagg atatatcatg ggtaaaacaa taagtatgaa gcttctgttc
     4681 ggtatttatc ttctattaat ggcaggcaaa gtttttgcct tcagttgtaa tgtcgatggc
     4741 gggtcaagta ttggagcagg tacaacctct gtttatgtta atctcgaccc tgtaatacaa
     4801 ccgggccaga atctggttgt agacttgtct cagcacataa gttgctggaa tgattacggc
     4861 ggctggtacg acactgatca tataaacctg gtacaaggct cagctttcgc aggctctcta
     4921 cagtcatata aaggatcgct gtactggaat aatgtgacct accccttccc gttgacgaca
     4981 aatactaatg tattagatat tggtgataag acaccgatgc cattaccgtt aaaactctat
     5041 attaccccag tcggtgcggc cggtggagtt gtcataaaag ccggggaagt tattgcccgt
     5101 attcatatgt ataaaattgc cacgttagga agtggaaatc cgcgtaattt tacgtggaat
     5161 attatttcaa ataacagtgt tgttatgcca acaggcggct gcaccgttga tagtcgtaat
     5221 gtcaacgtga atctcccgga ctttcctgga agcgcagaaa taccccttgg cgtatattgc
     5281 tcaagtgaac aaaagctctc attttatctt tcgggtgcta ccactgacag tgccagacaa
     5341 gtgtttgcta acacagcacc agatgcaaca aaagcatcgg gagtgggtgt ctctctgatg
     5401 cgtaatggaa aaatactggc tacgggagaa aacgtctccc tggggacagt aaataaaagc
     5461 aaagtacctc tgggttt
//
LOCUS       DACBVI010000228         5448 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.230, whole genome shotgun sequence.
ACCESSION   DACBVI010000228 DACBVI010000000
VERSION     DACBVI010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.230"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            206..1552
                     /locus_tag="IU325_21590"
     CDS             206..1552
                     /locus_tag="IU325_21590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811583.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKKPATMAQHNLA
                     NDLLREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSSGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAYHNGESSSVRKKRPRLA
                     RLLLSLIDDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRSLSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVV
                     IETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFEKHMYGVFMHLRY"
     gene            1831..3711
                     /locus_tag="IU325_21595"
     CDS             1831..3711
                     /locus_tag="IU325_21595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="HAP5811584.1"
                     /translation="MSDPVRITNPGAESLGYDSDGHEIMAVDIYVNPPRVDVFHGTPP
                     AWSSFGNKTIWGGNEWVDDSPTRSDIEKRDKEITAYKNTLSAQQKENENKRTEAAKRL
                     SAAIAAREKDENTLKTLRAGNADAADITRQEFRLLQAELREYGFRTEIAGYDALRLHT
                     ESRMLFADADSLRISPREARSLIEQAEKRQKDAQNADKKAADMLAEYERRKGILDTRL
                     SELEKNGGAALAVLDAQQARLLGQQTRNDRAISEARNKLSSVTESLNTARNALTRAEQ
                     QMTQQKNTPDGKTIVSPEKFPGRSSTNHSIVVSGDPRFAGTIKITTSAVIDNRANLNY
                     LLTHSGLDYKRNILNDRNPVVTEDVEGDKKIYNAEVAEWDKLRQRLLDARNKITSAES
                     AVNSARNNLSARTNEQKHANDALNALLKEKENIRNQLAGINQKIAEEKRKQDELKATK
                     DAINFTTEFLKSVSEKYGAKAEQLAREMAGQAKGKKIRNVEEALKTYEKYRADINKKI
                     NAKDRAAIAAALESVKLSDISSNLNRFSRGLGYAGKFTSLADWITEFGKAVRTENWRP
                     LFVKTEAIIAGNAATALVALVFSILTGSALGIIGYGLLMAVTGALIDESLVEKANKFW
                     GI"
     gene            complement(3733..4068)
                     /locus_tag="IU325_21600"
     CDS             complement(3733..4068)
                     /locus_tag="IU325_21600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 1A immunity protein"
                     /protein_id="HAP5811585.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYDFKYRLLFWCISLCGMV
                     LYPVAKWYIEDTALKFTRPDFWNSGFFADTPGKMGLLAVYTGTVFILSLPLSLIYILS
                     VIIKRLSVR"
     gene            4197..4544
                     /locus_tag="IU325_21605"
     CDS             4197..4544
                     /locus_tag="IU325_21605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811586.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLCMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(4564..5154)
                     /locus_tag="IU325_21610"
     CDS             complement(4564..5154)
                     /locus_tag="IU325_21610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811587.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQKRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVELNGMTYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGIEVRVQINESAAP"
     gene            complement(5151..5411)
                     /locus_tag="IU325_21615"
     CDS             complement(5151..5411)
                     /locus_tag="IU325_21615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001488293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811588.1"
                     /translation="MDEEKVFSLSYEQLTRFAEKRIRECNLDSQGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
BASE COUNT         1482 a         1267 c         1342 g         1357 t
ORIGIN      
        1 ttgatattcg actgaaccct gaccgaaaca tggttgatat attctctcac attaatgttg
       61 tcatgatata aataaaaaca attcatttga aaggatatca gaagaagata tcggggcgca
      121 cagttcatct ctgcctttgt ttcatggggt gttctttgta tattttactt actgtttaag
      181 catgtacata aggagtgctt tcattatgcc aatgatactt gtaaagaaga atccccgggg
      241 caaagtgatt cgggagctta gcagtgagga agagactgca gtaaagacgg tctgtggact
      301 gaaaaagcca gcaaccatgg cacaacataa cctggccaat gatcttctcc gggaaatgcg
      361 tgagtatgat gcatggttac agtgtgactg tattccggga gattcgccgg ccatgaattt
      421 tgctgctctt aaaaacaata cgggtactct ttatctgtcc agttttaatc atgagcatgc
      481 acctgaatgc cccatgtatc gtcagttatc cggaaatgaa gaggaaacat cttccggagc
      541 aagccggcat cccgttagca cccgtataaa ttacagaaac tttttaccac ctgatgacag
      601 taattctgtt atcagactgc aggcccgctc tgcttaccat aatggtgaga gctcctccgt
      661 tcgcaagaaa cgtccgcgcc ttgcccgttt actcctgtct cttattgacg atgctgggct
      721 gaataaactg gattccctgg ctaatccccg tatccggact aattatcgtg aatgtcttga
      781 tgccatacgg caggttacac tgcagcagga gtatattcgg ggaagatcct tatctgaaat
      841 tatccatttc cggcctggaa tgtctgaaag gtctcaggag cggttaatgg aaacgctgga
      901 gaattcagaa cgacactggc ctgccagaag aaaacatatg ttctttcaga tttttatggc
      961 gcagcacatc tgtcgtgatg ctgtggaaat tcactgggcg aacggtaata ttcaggtcat
     1021 tagacctgtg cgggggatca gtattaatgg tgaagcgcag ggcggaatac gtccccctta
     1081 ctgggttatt cttgcttttt gccggagtgc cgatggcaga atcatctgca gtgaagggta
     1141 tgctcatgct ctttatcaac tgacatgtcc ggtgcctgtg gacagcaaac tggaacgaaa
     1201 cacgctcact gctcttctga atgtggccag ctggcttaaa agaaagccag gtacgccgga
     1261 attaagtctg gaaaggcccc tgtttgatac agaagtttat gttaatggtg aaaagaaata
     1321 tgtcctgccg gatttcattg tcacagcaag ggctcctgac ggaaagacgg ccagagtggt
     1381 catcgaaacg atgggatatg aagacagtga ttactgcgcg agaaaatcca ggcagcatac
     1441 cggcatgaag cagattggtg ttctgcatac cgatccaccg aaatggctgg ataacgatca
     1501 tccccctttt gagaaacata tgtacggtgt ttttatgcat ctcaggtact gagatatttt
     1561 gtggctcagt tctgtaactt ttcccgtaac attgtctgtt gttacgggaa agtccggttt
     1621 ttgtattgca ccagagaata ctcagactgt gatgctgcca cagcgtcagc aggctttctg
     1681 aacggtgtaa catcacttca tgttaatgat aatcactatc attaaatctt gacatgccat
     1741 tttctcctta ataaattaat actgtatatg tatccatgta cgtaagcagt taattcattt
     1801 gttttcctca gaggatgaag gagataccga atgtctgacc ctgtacgtat tacaaatccc
     1861 ggtgcagaat cgctggggta tgattcagat ggccatgaaa ttatggccgt tgatatttat
     1921 gtaaaccctc cacgtgtcga tgtctttcat ggtaccccgc ctgcatggag ttccttcggg
     1981 aacaaaacca tctggggcgg aaacgagtgg gtcgatgatt ccccaacccg aagtgatatc
     2041 gaaaaaaggg acaaggaaat cacagcgtac aaaaacacgc tcagcgcgca gcagaaagag
     2101 aatgagaata agcgtactga agccgcaaaa cgcctctctg cggcgattgc tgcaagggaa
     2161 aaagatgaaa acacactgaa aacactccgt gccggaaacg cagatgccgc tgatattaca
     2221 cgacaggagt tcagactcct gcaggcagag ttgagagaat acggattccg tactgaaatc
     2281 gctggatatg atgccctccg gctgcataca gagagccgga tgctctttgc tgatgctgat
     2341 tctcttcgta tatctccccg ggaggccagg tcgttaatcg aacaggctga aaaacggcag
     2401 aaggatgcgc agaacgcaga caagaaggcc gctgatatgc ttgctgaata cgagcgcaga
     2461 aaaggtattc tggacacgcg gttgtcagag ctggaaaaaa atggcggggc ggcccttgcc
     2521 gttcttgatg cacaacaggc ccgtctgctc gggcagcaga cacggaatga cagggccatt
     2581 tcagaggccc ggaataaact cagttcagtg acggaatcgc ttaacacggc ccgtaatgca
     2641 ttaaccagag ctgaacaaca gatgacgcaa cagaaaaaca cgcctgacgg caaaacgata
     2701 gtttcccctg aaaaattccc ggggcgttca tcaacaaatc attctattgt tgtgagcggt
     2761 gatccgagat ttgccggtac gataaaaatc acaaccagcg cagtcatcga taaccgtgca
     2821 aacctgaatt atcttctgac ccattccggt ctggactata aacgcaatat tctgaatgac
     2881 cggaatccgg tggtgacaga ggatgtggaa ggtgacaaga aaatttataa tgctgaagtt
     2941 gctgaatggg ataagttacg gcaaagattg cttgatgcca gaaataaaat cacctctgct
     3001 gaatctgcgg taaattctgc gagaaataac ctcagtgcca gaacaaatga gcaaaagcat
     3061 gcaaatgacg ctcttaatgc cctgttgaag gaaaaagaga atatccgtaa ccagcttgcc
     3121 ggcatcaatc agaagatagc ggaagagaaa agaaaacagg atgaactgaa ggcaacgaaa
     3181 gacgcaatta atttcacaac agagttcctg aaatcagttt cagaaaaata tggtgcaaaa
     3241 gctgagcagt tagccagaga gatggccggg caggctaaag ggaagaaaat acgtaatgtt
     3301 gaagaggcat taaaaacgta tgaaaagtac cgggctgaca ttaacaaaaa aattaatgca
     3361 aaagatcgtg cagcgattgc cgcagccctt gagtctgtga agctgtctga tatatcgtct
     3421 aatctgaaca gattcagtcg gggactggga tatgcaggaa aatttacaag tcttgctgac
     3481 tggatcactg agtttggtaa ggctgtccgg acagagaact ggcgtcctct ttttgttaaa
     3541 acagaagcca ttatagcagg caatgccgca acggctcttg ttgcactggt cttcagtatt
     3601 cttaccggaa gcgctttagg cattatcggg tatggtttac tgatggctgt caccggtgcg
     3661 ctgattgatg aatcgcttgt ggaaaaagcg aataagttct ggggtattta aataaaatat
     3721 aagacaggct gtctatctta cagacagcct ttttataata acagaaagaa tatatatcag
     3781 acttaacgga agagataata tgaaaacagt acccgtataa acagcaagca atcccatttt
     3841 tccaggtgta tcagcaaaaa aaccgctgtt ccagaaatca ggccgggtaa atttcagagc
     3901 tgtatcttca atataccatt ttgcaaccgg atacaaaacc attccgcaga gagaaataca
     3961 ccagaacagt aatctgtatt taaaatcata atcccatgac atatacagca tatatccccc
     4021 cgtcacccat ccccaccaca tattattaaa ataatatttt ctgttcatct gcctgttttc
     4081 ctttcttttg cacttttgtc agtgtactga tgcatgacaa cataaccaca gcatgataat
     4141 aataatcaat aacaataagc tgtgtcacgt ttacatcatg gaacggaggt gctcctgtga
     4201 cctttgagca gaaaaaagcc cgggccattg ccctgatgga cagcaaaaag atgtggcgga
     4261 gcaattatgc ccctcctctt ctgcgtattc tatggcggct gggtatccgg cttccgccat
     4321 taccctttat gccgttctgg caggtcacgg tgctgacggg gggcttatgg ggcatttcct
     4381 ggggatgtgc aatgtggttt atctactggg ggccgtcagg aatggtggca ggtgaggcca
     4441 ttattataag tatcacgggt ggttttttgt tcggactgtg catggcctct ttccactggt
     4501 ggcgcaggaa agttaaccgg ctgccaccat gggatgatgt gtaaaccgtg cacaggcgtg
     4561 ttcttacgga gcggcacttt catttatctg tacgcgaact tctattcctg cgtcatgaca
     4621 ggcctgcagc cactgcgcca cttccagcgg atcgccctcc cggcgtacca ccctgccttc
     4681 tttattccat aactgcagac aggtgctgcc gtcgagacgc accacaaaat ccccacggca
     4741 ggcctgatac gtcatgccgt tcagctccac cgccagatct gtggcagaag tgccgtctgc
     4801 gggcagacct gctgccggga ccagcggcag tacgacgact tctgccgcac gctgttctgc
     4861 ccggaggcgc ttctgttctg ccagcagcag aggctgtgcc acggcccggc cggcgtccgt
     4921 cagctccact gccagctgca gattcggggc tcgcagggtg cgcagccagc cggcatcctc
     4981 caggcgacgg caggaggcgc gcaggtttgg cccgtagacc ggggcttccc cgcttcgctc
     5041 cagcacccgt tctatgtccc gcgtcagcac ggggcctggt cgtttttcat caagggcagc
     5101 cagcacaatc agcactctcc gctgcagtgg cgacggtcgg cggctctgag tcatttccag
     5161 tcatcctccg gccagatgag ttttctgagg cgctgatggt cggcgtcaat gatctcctgt
     5221 ctctttatgg cattcattga ggtatagcca tttatggcca gttcatgcca gaagatcagc
     5281 acggccccgg ctttggctga ctcgcagaga taagttacgc cctggctgtc cagattgcat
     5341 tcccggatgc gtttttccgc aaaccgggtc agttgttcat agctgagtga aaacaccttt
     5401 tcttcatcca tcctgcacgc tccatctgat aatgttctga caggtaaa
//
LOCUS       DACBVI010000229         5432 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.231, whole genome shotgun sequence.
ACCESSION   DACBVI010000229 DACBVI010000000
VERSION     DACBVI010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.231"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..1201
                     /gene="fes"
                     /locus_tag="IU325_21620"
     CDS             <1..1201
                     /gene="fes"
                     /locus_tag="IU325_21620"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HAP5811589.1"
                     /translation="TALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYST
                     IKRVWVYITGVTDHHQNSQPQSMQRIAGTNVWQWTTQLNANWRGSYCFIPTERDDIFS
                     APSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQAP
                     ETPAKEIIWKSEWLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSLT
                     HRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTVV
                     AGQSFGGLSALYAGLNWPERFGCVLSQSGSYWWPHRGGQQEGVLLEQLKTGEVSAEGL
                     RIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLWQ
                     PLFHDRS"
     gene            1204..1422
                     /locus_tag="IU325_21625"
     CDS             1204..1422
                     /locus_tag="IU325_21625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family NRPS accessory protein"
                     /protein_id="HAP5811590.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDVVCQPQ
                     SQASCQQWLEAHWRTLTPTNFTQLQEAQ"
     gene            1419..5300
                     /gene="entF"
                     /locus_tag="IU325_21630"
     CDS             1419..5300
                     /gene="entF"
                     /locus_tag="IU325_21630"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HAP5811591.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDAQTFELPEIIDLRTNIDPHGTARALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALVALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIEAQETLPQLATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYVQLAQINATQV
                     EIPETTLSALVAEQAAKTPHAPALVDASYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTRLCYNAPLTPQGSAPLKLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTSV
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIANPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTTALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFTRQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPNGPMQTAANLDEVCEAHLATLLEHQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMNPERVWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
BASE COUNT         1168 a         1428 c         1586 g         1250 t
ORIGIN      
        1 gacggcgtta aaagtaggaa gtgagagctg gtggcagtcg aaacatggcc cggaatggca
       61 gcgtctgaat gacgaaatgt ttgaggtcac tttctggtgg cgtgatcccc agggttctga
      121 agaatactcg acgataaagc gcgtatgggt ctacatcact ggtgtgaccg atcaccatca
      181 gaacagccag ccccagtcga tgcagcgaat tgcaggtacc aacgtctggc agtggacgac
      241 acaacttaat gccaactggc gcggcagcta ctgttttatt cccaccgaac gcgatgacat
      301 tttttctgca ccatcccccg atcgcctcga attacgtgaa ggctggcgaa aactattgcc
      361 ccaggcgata gccgatccgc tgaatccaca aagctggaaa ggcgggcgag ggcacgctgt
      421 ttctgcactc gaaatgccgc aagcgcccct gcaaccgggt tgggattgtc cgcaagcgcc
      481 agaaacacct gccaaagaaa ttatctggaa aagtgaatgg ttgaaaaatt cacgtcgtgt
      541 atggattttt accaccggcg atgtaacagc agaagaacgc ccgctggcag ttttgctcga
      601 tggcgaattt tgggcgcaaa gtatgcccgt ctggcctgtg ctgacttcgc tgacccatcg
      661 tcagcaactt cctcccgccg tgtatgtgtt gatcgacgct atcgacacca cgcaccgcgc
      721 ccacgaactg ccgtgtaatg cggatttctg gctggcagta cagcaagagt tattacccct
      781 ggtgaaagct attgcccctt ttagcgatcg cgccgatcgc accgtggtcg ccgggcagag
      841 ttttggtggg ctttccgcgc tgtatgccgg actaaactgg cctgaacgct ttggctgtgt
      901 attaagccag tcaggatcat actggtggcc gcatcgaggc gggcagcaag agggcgtgtt
      961 acttgaacag ctaaaaactg gtgaagttag cgccgaaggt ctgcgcattg tgctggaagc
     1021 gggtattcgc gagccgatga tcatgcgggc caatcaggcg ctgtatgcgc aattacaccc
     1081 cataaaagaa tccattttct ggcgtcaggt tgacggcgga catgatgcgc tttgttggcg
     1141 cggtggcttg atgcaggggc taatcgacct ctggcaacca cttttccatg acaggagttg
     1201 aatatggcat tcagtaatcc cttcgatgat ccgcagggag cgttttacat tttgcgcaat
     1261 gcgcaggggc aattcagtct gtggccgcaa caatgcgcct taccggcagg ctgggacgtt
     1321 gtgtgtcagc cgcagtcaca ggcgtcctgc cagcagtggc tggaagccca ctggcgtact
     1381 ctgacaccga cgaatttcac ccagttgcag gaggcacaat gagccagcat ttacctttgg
     1441 tcgccgcaca gcccggcatc tggatggcag aaaaactgtc ggatttaccc tccgcctgga
     1501 gcgtggcgca ttacgttgag ttaaccggag aggttgatgc gccattactg gcccgcgcgg
     1561 tggttgccgg attagcgcaa gcagatacgc tgcggatgcg ttttacggaa gataacggcg
     1621 aagtctggca gtgggtcgat gatgcgcaga cgttcgaatt gccagaaatt atcgacctgc
     1681 gaaccaacat tgatccgcac ggtactgcgc gggcattaat gcaggcggat ttgcaacaag
     1741 atttgcgcgt cgatagcggt aaaccactgg tctttcatca gttgatacag gtggcggata
     1801 accgctggta ctggtatcag cgttatcacc atttgctggt cgatggcttc agtttcccgg
     1861 ccattacccg ccagatcgcc aatatttact gcgcatggct gcgtggcgaa ccaacgcctg
     1921 cttcgccgtt tacgcctttc gctgatgtag tggaagagta ccagcaatac cgcgaaagcg
     1981 aagcctggca gcgtgatgcg gcattctggg cggaacagcg tcgtcaattg ccgccgcccg
     2041 cgtcactttc tccggcacct ttaccggggc gcagcgcttc ggcagatatt cttcgcctga
     2101 aactggaatt tactgacggg gaattccgcc agctggctac gcaactttca ggtgtgcagc
     2161 gtaccgattt agcccttgcg ctggtggcct tatggctggg gcgattgtgc aatcgcatgg
     2221 actacgccgc cggatttatc tttatgcgtc gactgggctc ggcggcgctg acggctaccg
     2281 gacccgtgct caacgttttg ccgttgggta ttcatattga ggcgcaagaa acgctgccgc
     2341 aactggcaac ccgactggca gcacaactga aaaaaatgcg tcgtcatcaa cgttacgatg
     2401 ccgaacaaat tgtccgtgac agcgggcgag cggcaggtga tgaaccgctg tttggtccgg
     2461 tactcaatat caaggtattt gattaccaac tggatattcc tggtgttcag gcgcaaaccc
     2521 ataccctggc aaccggtccg gttaatgacc ttgaactggc actgttcccg gatgaacacg
     2581 gtgatttgag tattgagatc ctcgccaata aacagcgtta cgatgagcca acgttaattc
     2641 aacacgctga acgcctgaaa atgctgattg cccagttcgc cgcggatccg gcgctgttgt
     2701 gcggcgatgt cgatattatg ctgccaggtg aatatgtgca gctggcgcag atcaacgcca
     2761 ctcaggttga gattccagaa accacgctga gcgcgctggt ggcggaacag gcggcaaaaa
     2821 caccgcatgc tccggcgctg gttgatgcga gttaccagtt tagctatcgg gaaatgcgcg
     2881 agcaggtggt ggcgctggcg aatctgctgc gtgagcgcgg cgttaaacca ggcgacagcg
     2941 tggcggtggc gctaccgcgc tcggtctttt tgactctggc gctacatgca attgttgagg
     3001 ctggtgcggc ctggttaccg ctggataccg gctatccgga cgatcgcctg aaaatgatgc
     3061 tggaagatgc gcgtccgtcg ctgttaatca ccaccgacga tcaactgccg cgctttgccg
     3121 atgttccaga tttaacccgc ctttgctata acgccccgct tacaccgcag ggcagtgcgc
     3181 cgctgaaact ttcacaaccg caccacacgg cttatatcat ctttacctct ggctccaccg
     3241 gcaggccgaa aggggtaatg gtcgggcaga cggctatcgt taaccgcctg ctttggatgc
     3301 aaaaccacta cccgcttaca ggcgaagatg tcgttgccca aaaaacgccg tgcagttttg
     3361 atgtctcggt gtgggagttt ttctggccgt ttattgccgg ggctaaactg gtgatggctg
     3421 aaccggaagc gcaccgcgac ccgctcgcta tgcagcaatt ctttgccgaa tatggcgtaa
     3481 cgactacgca ctttgtgccg tcgatgctgg cggcatttgt tgcatcgctg acgccgcaaa
     3541 ccgctcgcca gaattgcgcg acgttgaaac aggttttctg tagtggtgag gccttacccg
     3601 ctgatttatg ccgcgaatgg caacagttaa ccagcgtgcc gttgcataat ctatatggcc
     3661 cgacggaagc ggcggtagat gtcagctggt atccggcttt tggcgaggaa ctggctcagg
     3721 tgcgcggcag cagtgtgccg attggttatc cggtgtggaa tacgggcctg cgtattcttg
     3781 atgcgatgat gcatccggtg ccgccgggtg tggcgggaga tctctatctc actggcatac
     3841 aactggcgca gggctatctc ggacgacccg atctgaccgc cagccgcttt attgccaatc
     3901 cttttgcccc tggcgaacgg atgtaccgta ccggagacgt tgcccgctgg ctggataacg
     3961 gcgcggtgga gtacctcggg cgcagtgacg atcagctaaa aattcgcggg cagcgtatcg
     4021 aactgggcga aatcgatcgc gtgatgcagg cgctgccgga cgttgaacag gccgtgaccc
     4081 acgcctgcgt tattaaccag gctgccgcaa cgggaggcga tgcgcgtcag ttggttgggt
     4141 atctggtgtc gcaatcaggc ctgccgttgg ataccactgc attgcaggcg cagttgcgcg
     4201 aaacattgcc accgcatatg gtgcccgtgg ttctgctgca acttccgcag ttgccgctta
     4261 gcgccaacgg caagctggat cgcaaagcct taccgttgcc tgaactgaag gcacaagcgc
     4321 cggggcgtgc gccgaaagcg ggcagtgaaa cgattatcgc cgcggcattc tcgtcgttgc
     4381 tgggttgtga cgtgcaggat gccgatgctg atttcttcgc gcttggcggt cattcgctac
     4441 tggcaatgaa actggctgcg cagttaagtc ggcagtttac ccgccaggtg acgccggggc
     4501 aggtgatggt cgcgtcaact gtcgccaaac tggcaacgat tattgatggt gaagaagaca
     4561 gctcccggcg catgggattc gaaaccattc tgccgttgcg tgaaggtaat ggcccgacgc
     4621 tgttttgttt ccatcctgcg tccggttttg cctggcagtt cagcgtgctc tcgcgttatc
     4681 tcgatccaca atggtcgatt atcggcattc agtcgccgcg ccccaatggc cccatgcaga
     4741 ccgcggcaaa tctggatgaa gtctgcgaag cgcatctggc aacgttactt gagcatcaac
     4801 cgcacggccc ttattacctg ctgggttatt cgctgggcgg tacgctggcg cagggtattg
     4861 cggcgcggct gcgtgcccgt ggcgaacagg tggcatttct tggcttgctg gatacctggc
     4921 cgccagaaac gcaaaactgg caggaaaaag aggctaatgg cctggacccg gaagtgctgg
     4981 cggagattaa ccgcgagcgc gaggccttcc tggcggcaca gcagggaagt acttcaacgg
     5041 agttgtttac caccattgaa ggcaactacg ctgatgctgt gcgcctgctg acgactgctc
     5101 atagcgtacc gtttgacggt aaagcgacgc tgtttgttgc tgaacgcacg cttcaggaag
     5161 gtatgaatcc cgaacgcgtc tggtcgccgt ggatagccga gctggatatc tatcgtcagg
     5221 attgtgcgca tgtggatatt atctctccag gggcatttga aaaaattggg ccgattattc
     5281 gcgcaacgct aaacagataa attaacatta tctataaacc cataattaca gaaaataatt
     5341 atgggttttt tatttgtttg atttataggt ttgatgaata tttctcttaa atagagtgaa
     5401 tgttgcattc agtggaagga atttacaaac tt
//
LOCUS       DACBVI010000230         5383 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.232, whole genome shotgun sequence.
ACCESSION   DACBVI010000230 DACBVI010000000
VERSION     DACBVI010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.232"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(99..1382)
                     /gene="aroA"
                     /locus_tag="IU325_21635"
     CDS             complement(99..1382)
                     /gene="aroA"
                     /locus_tag="IU325_21635"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP5811592.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALAALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGSFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1453..2541)
                     /gene="serC"
                     /locus_tag="IU325_21640"
     CDS             complement(1453..2541)
                     /gene="serC"
                     /locus_tag="IU325_21640"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HAP5811593.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNIPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVAAMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2740..3432)
                     /locus_tag="IU325_21645"
     CDS             complement(2740..3432)
                     /locus_tag="IU325_21645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP5811594.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3562..5322
                     /locus_tag="IU325_21650"
     CDS             3562..5322
                     /locus_tag="IU325_21650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HAP5811595.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1370 a         1383 c         1291 g         1339 t
ORIGIN      
        1 cattaatcaa aatgactcac aaggtccgaa aaaaaacgcc ggcaatgtgc cgacgttttg
       61 ataaagaaag atttggctat ttattgcacg ttgttcattc aggctgcctg gctaatccgc
      121 gccagctgct cgaaataatc cggaaatgtt ttggccgtgc atttgggatc aagaatcgtc
      181 actggtgtat ctgacaacgc caccagcgag aaacacatcg ccatccggtg atcattgtat
      241 gttgcgatct cggcaaagtt cagtttttcc ggtggagtga tacgaatgta atcgtgcccc
      301 tcttctactt ccgcaccaac tttacgcagt tctgttgcca tcgcaaacag gcgatcggtc
      361 tctttaacac gccagttata gatattgcgc agcgtggtgg tgccttttgc aaataacgcc
      421 gccgtggcaa tggtcatcgc cgcatcagga atatggttca tatccatatc aatagcgttc
      481 agttcacccc gcgtgcagga aatataatca tcgccccagc aaatggtcgc gcccattttt
      541 tccagcacat cagcaaagcg aatatcaccc tgcatactgt tgcgtccaat gccggtcact
      601 tttacagtgc cgcctttgat tgctgctgct gccaggaagt aagaagccga agatgcatcg
      661 ccttcgacca aataagtacc cggagactga taagactgcc cgccttttac gacaaattgt
      721 tgatagtgct gattttcaat ttcaacacca aacgtcttca tcagattgag tgtgatgtcg
      781 atataaggtt tagaaaccag atcgccttta atacgaataa ccgtatcttc cggcgcaaga
      841 ggcgcagtca ttaatagtgc ggtgaggaac tggctggaaa cggagccatc aacgtcaacg
      901 ttgccgccag taaagctgcc ctgtaaacgc aatggcggat aattttcttg ttccaggtaa
      961 gtgatcttcg ccccgcccag gcgcagcgca tccaccagat gaccaatcgg gcgctctttc
     1021 atacgcggct caccagtcag cacaatatca ttgctaccca gacaaagagc tgccgccagc
     1081 gggcgcattg ccgttccggc gtttccgagg aacaactcca gggcaccttc tgcgtgtaat
     1141 ggaccgccgt taccgataat ttcgcaacgc gtacgatcag ctgaaagcgt atagcttacc
     1201 cctaacgctg ctaatgcatt cagcatatgg cgcacgtcat cgctatccag cagattggtt
     1261 aatactgttt tgccgtgtgc taatgccgcc agcaataaag cgcggttaga aacgctcttg
     1321 gaaccgggca gattaatagt gccatcgaca cgagcgatgg gttgtaacgt cagggattcc
     1381 atgaactcaa ctctctacaa cagaaataaa aaccccacag gctggctgtg gggattaagc
     1441 aaaatttcgg cattagccgt gacggcgttc gaactcaacc atgaagtctg tcagcgcttt
     1501 aacgccttcc agcggcatgg cgttataaat agatgcgcgc attccgccga ccacacggtg
     1561 acctttcagc gcatgaaggc cagcagcaaa agactcttca aggaacaatt tgtcaagcgc
     1621 actgtccgcc aactggaacg gcacgttcat ccgcgaacgg ttagctttcg ccacgtcatt
     1681 gcggtagaaa tcgctgttat caatcacccc atacagcagt tctgcttttt gctgattgat
     1741 tttatccatt gcagctacac cgccgttcgc tttcagccat ttaaagacca gaccagacag
     1801 ataccaggca aatgtcggcg gcgtgttaaa catggagtcg ttatcgttga gaatggaata
     1861 atccagaatc gacggacacg cgatattcgc tttgcccagc aaatcttcac gaacgatgac
     1921 gattgtcagg ccagccgggc cgatattttt ctgcgcgcca gcgtaaatta caccataacg
     1981 gctgacgtca atcggacggg aaagaatggt tgaagagaag tcggcggcga ccaccacatc
     2041 tttgccgaag tctggcgttt cgtcgatagc gataccgtcg atggtttcat tcgggcagta
     2101 atgcatataa gcagcattat cagagagttg ccattcgcgc attggcttaa ccgcgcgcag
     2161 accatcaaca gtcactttgg cgtcgaagac attaggcgtg caatattttt tcgcttcttt
     2221 aatggcactt gccgcccagt aaccggcatc aacataatct gcggtggttt tatcaccgag
     2281 aatattcagc ggaacagcag caaactgacc gcgaccgccg ccatggcaga acaatacttt
     2341 gtagttggag gggatgttaa gaagatcgcg aaaatccttc tcggcttcct ctgcaacctg
     2401 aatgaactct ttgccccggt gactcacttc catcaccgac gtaccaagac cgttccagtc
     2461 acgcagttcc tgttgagcct gtttaagcac ctctgccggt agcattgccg gaccagaact
     2521 aaaattgaag atttgagcca tttcccctca ccacgttgcg ttgctattaa cccccggtca
     2581 atcgattgcg accgcgggta atatgttatt cctgtcgcta tcggttttat cattcagtga
     2641 cacgcatcgc aatgggtaaa agcgttgagg gcaaaaatgc ggcacgcgtc acacaacaga
     2701 atctctcgga ttattaccaa aaaaccgata tttttgctgt caggtcaccc gtttagctcg
     2761 acttgccttc gtccattctg gatttgcaca gcgcggacat aattgttttt cccccgcgtt
     2821 catatgaatg atttcactgc aacggatgca ggcatagtcc gccgactccg gcaccacttt
     2881 gtaacgctga gtacagtcac tgggtaaaat aagtaagccc ggtttcacct tgtcatcttc
     2941 aaagaaataa acactgattt gcccgttggt ttcgagaatc gccagacgta cttgccccaa
     3001 ctgctctaca ccgcgcaatc gtagctccat aaagaactca aattccgtca tgttggagtt
     3061 attgagtttc gaccaggcca gctcgccatc ttcaataatg acaaccggct taccttccag
     3121 aagatcttcc agtttttcac tgtgtgccat caaccacatt accaggcggt ataacaacgc
     3181 cagagtgata aagacgataa gtaccgggac catcggcaca tcatcataaa acgccacatc
     3241 tcccgccgct gaccccagcg tcagaatgat taacacttca aacagcgaca tctgccgcac
     3301 tccgcgtctt ccggtcattt tgaggaacaa aaaaaccagt acaaaggtat acagactacg
     3361 tagtgccact tcgccaagga aatcaaaagg cactttatca aatgccatac ggtggagatc
     3421 gaacgccttc atttgtatgc tcttatcgtg gtttatccgc taaaagcata gtcaacagat
     3481 catcggcatg gcggcggtga tagcgccttt gagataaaac ccgtattatt gcgcgctttc
     3541 cgtacgacta aagtgatttt catgacgcaa acatttatcc ccggcaaaga tgccgcactg
     3601 gaagattcca tcgctcgctt ccagcaaaaa ctttcagacc tcggctttca gattgaagag
     3661 gcctcctggc tgaatcccgt gcctaacgtc tggtctgtac atattcgcga caaagagtgc
     3721 gcactgtgtt ttaccaacgg taaaggcgca accaaaaaag cggcgctggc ttctgcactc
     3781 ggtgaatatt tcgagcgtct ctcaaccaac tacttttttg ctgatttctg gctgggcgaa
     3841 accatcgcca acggtccgtt cgtgcattat cccaacgaaa aatggttccc actgagcgaa
     3901 aatgacgatg tgccagaagg gctgctcgat gaccgtctgc gcgcatttta cgatccggag
     3961 aatgaattga ccggcagtat gctgattgac ctgcaatccg gtaacgaaga tcgtggtatt
     4021 tgcggtctgc cgtttacgcg ccagtccgac aatcagaccg tttatattcc gatgaatatc
     4081 attggtaacc tgtacgtttc taacggtatg tccgctggca atacccgcaa cgaagcacgc
     4141 gttcaggggt tgtccgaagt tttcgaacgc tacgtgaaaa accgcattat tgctgaaagc
     4201 atcagcttgc cggaaatccc ggcagacgtg ctggcgcgtt acccagcggt ggttgaagcc
     4261 atcgaaacgc tggaagcaga aggtttccca atctttgctt atgatggttc gcttggcggc
     4321 cagtatccgg tgatttgcgt ggtactgttt aatcctgcta acggcacctg ctttgcctct
     4381 ttcggtgcgc atcctgattt tggcgtagca ctggaacgta ccgtgaccga gctgctgcaa
     4441 ggtcgtggcc tgaaagattt ggatgtgttt actccgccaa ccttcgatga tgaagaagtt
     4501 gctgaacata ccaacctcga aacgcacttt atcgattcca gcggtttaat ctcctgggac
     4561 ctgttcaagc aggatgccga ttatccgttt gtggactgga atttctccgg caccacggag
     4621 gaagagtttg ctacgctgat ggctatcttc aacaaagaag ataaagaagt ttatattgcc
     4681 gattacgagc atctgggcgt ttatgcttgc cgaattatcg tgcctggcat gtccgatatt
     4741 tatccggctg aagatctgtg gctggcgaat aacagtatgg gcagccattt acgtgaaacg
     4801 attctttcgt taccaggcag cgagtgggaa aaagaagatt acctgaacct catcgagcaa
     4861 ctggatgaag aaggttttga tgactttacc cgcgtgcgtg agctgttggg tctggcgacc
     4921 gggtcggata acggttggta caccctgcgt atcggtgaat taaaagccat gctggcgctg
     4981 gctggtggcg atctggaaca ggctctggtc tggaccgaat ggacgatgga gtttaactca
     5041 tcagtattta gtccggaacg cgccaactat tatcgctgcc tgcaaacgtt gttattactg
     5101 gcgcaggaag aagatcgcca gccgctgcaa tatttgaatg cgtttgttcg catgtacggc
     5161 gcagatgccg tggaagccgc cagtgcggca atgagcggcg aagcggcgtt ttatggcttg
     5221 caaccagtag atagcgatct gcacgcgttt gcagcacatc agtcgttgtt gaaggcctac
     5281 gaaaagctgc agcgcgccaa agcagcattc tgggcaaaat aaaatcaaat agcctacgca
     5341 atgtaggctt aatgattcgt ctgagttata ttacggggcg ttt
//
LOCUS       DACBVI010000231         5377 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.233, whole genome shotgun sequence.
ACCESSION   DACBVI010000231 DACBVI010000000
VERSION     DACBVI010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.233"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            133..1203
                     /gene="dhaK"
                     /locus_tag="IU325_21655"
     CDS             133..1203
                     /gene="dhaK"
                     /locus_tag="IU325_21655"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP5811596.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEESQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            1214..1846
                     /gene="dhaL"
                     /locus_tag="IU325_21660"
     CDS             1214..1846
                     /gene="dhaL"
                     /locus_tag="IU325_21660"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HAP5811597.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPAALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            1857..3275
                     /gene="dhaM"
                     /locus_tag="IU325_21665"
     CDS             1857..3275
                     /gene="dhaM"
                     /locus_tag="IU325_21665"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HAP5811598.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGAEIDKVIFDAMHALEAKREQLGLPSSNTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPILRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVDEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTILQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFSRQG"
     gene            3594..5291
                     /gene="treA"
                     /locus_tag="IU325_21670"
     CDS             3594..5291
                     /gene="treA"
                     /locus_tag="IU325_21670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreA"
                     /protein_id="HAP5811599.1"
                     /translation="MKSPTPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAIANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRASKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPVKQNEAEPTP"
BASE COUNT         1391 a         1360 c         1378 g         1248 t
ORIGIN      
        1 gtttcactat gaatcataat ttgggctaat tttcttttcc cttgcctgat gcacaatgga
       61 ttcatcggca gtcagtggtc gccgtgtcgt tgaacatcat ccatgcccta ccgtaattgc
      121 tggagcaaaa taatgaaaaa attgatcaat gatgtgcaag acgtactgga cgaacaactg
      181 gcaggactag cgaaagcgca tccatcgctg acactgcatc aggatccggt gtatgtcacc
      241 cgagctgatg cccccgttgc aggtaaagtc gccctgctgt cgggtggcgg cagcggacac
      301 gagccgatgc attgtggtta tatcggtcag gggatgcttt cgggggcctg tccgggcgaa
      361 attttcacct caccgacgcc cgataaaatc tttgaatgcg ccatgcaaat tgatggcggc
      421 gaaggtgtac tgttgattat caaaaattac accggcgata ttcttaactt cgaaacagca
      481 accgagttac tgcacgatag cggcgtaaaa gtgaccactg tggtcattga tgacgacgtt
      541 gcagtaaaag acagtcttta taccgccggg cggcgcggcg ttgccaacac cgtattaatt
      601 gaaaaactcg taggcgcagc ggcggagcgt ggcgactcac tggacgcctg tgcggaactg
      661 gggcgtaagt tgaataatca aggccactca ataggtattg ctctcggtgc ctgtaccgtt
      721 cctgccgcgg gcaaaccttc ttttaccctg gcggacaatg agatggagtt tggcgtcggc
      781 attcatggtg agccgggtat tgaccgacgc cccttctctt ctcttgatca aaccgtcgat
      841 gaaatgttcg acaccctgct ggaaaatggc tcataccatc gcactttgcg tttctgggat
      901 tatcaacaag gcagctggca ggaagaatca caaaccaaac aaccgctcca gtctggcgat
      961 cgggtgattg cgctggttaa caatcttggc gcaactccgc tttctgagct gtacggcgtc
     1021 tataaccgcc tgaccacacg ttgccagcaa gcgggattga ctatcgagcg taatttaatt
     1081 ggcgcgtact gcacctcact ggatatgacc ggtttctcaa tcaccttact gaaagttgat
     1141 gacgaaacgc tggcactctg ggacgccccg gtccacaccc cggcccttaa ctggggtaaa
     1201 taaggagata gcaatgtcac tgagcagaac tcaaattgtt aactggctca ctcgttgtgg
     1261 cgatattttc agcaccgaga gcgagtatct taccggactg gatcgcgaaa ttggcgacgc
     1321 tgaccacggg ctaaatatga accgaggctt tagcaaagtg gtggaaaaac tccctgctat
     1381 cgcagataaa gatatcggtt tcattctcaa gaataccggt atgacgctac tttccagcgt
     1441 cggtggtgcc agtggtccgc ttttcggtac cttctttatc cgcgccgcac aggcgaccca
     1501 ggcacggcaa agcctgacgc tggaagagct ttatcagatg ttccgcgatg gcgcggacgg
     1561 cgtaatcagt cgcggtaaag ccgaaccagg cgataaaacc atgtgtgatg tgtgggtgcc
     1621 ggtggtggaa tcgttacgtc agtccagcga gcaaaatctc tctgttccgg cggcgctcga
     1681 agctgccagt agcatcgccg agtccgctgc acaaagtacg attacgatgc aagcccgcaa
     1741 aggccgcgcc agttatctcg gtgaacgcag tattggtcac caggaccccg gtgcgacctc
     1801 ggtgatgttt atgatgcaaa tgttggcgtt agccgcaaaa gagtaaggaa ttggtgatgg
     1861 taaacctggt catagtttca catagcagcc gactgggaga aggtgtcggt gaactagccc
     1921 gtcagatgtt aatgagtgat agttgtaaaa tcgccattgc cgcgggaatt gacgatccac
     1981 aaaatcccat tggtaccgat gccgtcaaag tgatggaggc catcgaatct gttgctgatg
     2041 ccgaccatgt gctggtcatg atggatatgg gtagcgcatt attgagtgct gaaactgcgc
     2101 tggaattgct ggctcccgag atcgccgcaa aagtacgttt gtgtgctgca ccgttggtcg
     2161 aaggtacact ggcagcaacg gtcagcgcgg cctcgggggc ggagatcgac aaagttatct
     2221 ttgacgccat gcatgcgctg gaagccaaac gtgaacaact gggtttaccg tcctccaaca
     2281 ctgaaatctc tgacacatgt cctccgtacg atgaagaagc ccgttctctg gcggtggtca
     2341 taaaaaaccg taacggcctg catgtacgtc cggcctcccg gctggtttat accttatcga
     2401 catttaatgc cgatatgttg ctggagaaaa acggcaaatg cgtcacaccg gagagtatta
     2461 accagattgc gttactacaa gttcgctata acgatacgct gcgcctgatt gcgaaagggc
     2521 cagaagctga agaggcactg atcgctttcc gtcagctggc tgaagataac tttggtgaaa
     2581 cggaggaagt tgctccacct attctgcgtc ccgttccgcc tgtttcgggt aaagcctttt
     2641 attatcaacc ggttttatgt acggtacagg caaaatcaac cctgaccgtg gatgaagaac
     2701 aagaacgatt acgccaggcc attgacttca cgttattaga tctgatgacg ttaacagcga
     2761 aagcagaagc cagcgggctt gacgatattg ccgcaatctt ttctggtcac catacactgt
     2821 tagatgatcc ggaactgctg gcggcggcaa gcgaactcct tcagcatgaa cattgcacgg
     2881 cagaatatgc ctggcagcaa gttcttaaag aacttagcca gcaataccag caactggatg
     2941 atgaatatct acaggctcgc tatattgatg tggacgatct tctgcatcgc accctggtcc
     3001 acctgaccca aacgaaagaa gaactcccgc agtttaactc gccaactatt ctactggcgg
     3061 agaacatcta tccctccaca atactgcaac tggatccggc ggttgtaaaa ggtatctgcc
     3121 ttagcgccgg aagtccgcta tcccacagcg ccctgatcgc ccgtgaactg gggattggct
     3181 ggatttgcca gcagggtgag aaactgtatg cgatacaacc agaagaaacg ctaacgctgg
     3241 acgttaaaac gcaacgtttc agccgtcagg gttaaaccac agttcggatg gcatcgttct
     3301 gatgtcatcc ggcaatttat tctgcacgga tgtttttaac tacctgttat atttttcacc
     3361 aatttgtgat gatttttctt cgctaatcgc gaacccaacg ccgctggctg gctaacaaac
     3421 agacgttatt tgatcactct atttcgccgt tatttatccc tttaattccc ttttctaaaa
     3481 tgcctgaccg ttcgcagaat gagatttcga tcatgcagct agtgcgatcc tgaactaagg
     3541 ttttctgata cttgaatacc gttttattcg gtttcgccaa aggagaatga ttgatgaaat
     3601 cccccacccc ttctcgcccg caaaaaatgg cgttaattcc agcctgcatc tttttgtgtt
     3661 ttgctgcgct atcggtgcag gcagaagaaa caccggtaac gccacagccg cctgatattt
     3721 tattagggcc gctgtttaat gatgtgcaaa acgccaaact ttttccggac caaaaaacct
     3781 ttgctgatgc cgtgccgaac agcgatccgc tgatgatcct tgctgattat cggatgcagc
     3841 aaaaccagag cggatttgat ctgcgccatt tcgttaacgt caattttacc ctgccgaaag
     3901 agggcgagaa atatgttccg ccagaggggc agtcactgcg cgaacatatt gacggacttt
     3961 ggccggtatt aacgcgttct accgaaaaca ccgaaaaatg ggattctctg ttaccgctgc
     4021 cggaacctta tgtcgtgcca ggcggacgtt ttcgcgaggt atattactgg gacagttact
     4081 tcaccatgtt aggacttgcc gaaagcggtc actgggataa agtcgcggat atggtggcca
     4141 attttgctca tgaaatagac acttacggtc atattcccaa cggcaaccgc agttactatt
     4201 taagtcgctc gcagccgccc ttctttgccc tgatggtaga gttactggcg cagcatgaag
     4261 gcgatgccgc gttgaagcaa tacctgccgc aaatgcaaaa agaatatgct tactggatgg
     4321 acggtgttga aaacctgcaa gccggacaac aggaaaaacg cgttgtaaaa cttcaggatg
     4381 gtacccttct caaccgctac tgggacgatc gcgatacgcc acgaccagag tcatgggtgg
     4441 aagatattgc caccgccaaa agcaatccga atcgacctgc cactgaaatt taccgcgacc
     4501 tgcgctctgc cgctgcgtct ggctgggatt tcagctcgcg ctggatggac aacccgcagc
     4561 agttaaatac cttacgcacc accagcatcg taccggtcga tctgaacagc ctgatgttta
     4621 aaatggaaaa aatcctcgcc cgcgccagca aagctgccgg agataacgcg atagcaaacc
     4681 agtacgaaac gctggcaaat gcccgtcaaa aagggatcga aaaatacctg tggaacgatc
     4741 aacaaggctg gtatgccgat tacgacctga aaagtcataa agtgcgcaat cagttaaccg
     4801 cggccgccct gttcccgctg tacgtcaatg cggcagcgaa agatcgcgcc agcaaaatgg
     4861 cgacggcgac gaaaacacat ctgctgcaac caggcggcct gaacaccacg tcggtgaaaa
     4921 gtgggcaaca atgggatgcg ccaaacggct gggcaccgtt gcagtgggtc gcgacagaag
     4981 ggttacaaaa ctacgggcaa aaagaggtgg cgatggacat tagctggcac ttcctgacca
     5041 atgttcagca cacctatgac cgggagaaaa agctggtgga aaaatatgat gtcagcacca
     5101 ccggaacagg gggcggcggt ggcgaatatc cattacagga tggctttggc tggaccaatg
     5161 gcgtgacgct gaaaatgctg gatttgatct gcccgaaaga gcaaccgtgt gacaatgttc
     5221 cggcgacgcg tccactcagt gaatcaacaa cacagccggt aaaacaaaat gaggcggaac
     5281 ctacgcctta actttactgt atatcgagca atgccattac gtgcataaaa ttggcattgc
     5341 tctttcttca ccacgaaata ccaaatacca ctctttc
//
LOCUS       DACBVI010000232         5316 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.234, whole genome shotgun sequence.
ACCESSION   DACBVI010000232 DACBVI010000000
VERSION     DACBVI010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.234"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..593)
                     /locus_tag="IU325_21675"
     CDS             complement(<1..593)
                     /locus_tag="IU325_21675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP5811600.1"
                     /translation="MQMKSLHYGIFASITLSLLLFSTFSIAITDNIGLNVMTTVENRT
                     CQSEISNQGLVNLGTVGVGYFASNVSAEDNYPGGKTFTVTVKDCPSAGTTSSQLHIEF
                     KPVSGVMATGSQQIFANNHVGGAQNVGIVIFSQNNQSSDTVNVLSPTGQFRTVWPVAS
                     ANMNNSSWQFYTRMQKIDPLLPVGAGFVTSNVLVDIY"
     gene            complement(607..1167)
                     /locus_tag="IU325_21680"
     CDS             complement(607..1167)
                     /locus_tag="IU325_21680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP5811601.1"
                     /translation="MIKNTRYKIVLLAGIFLLPGALAQDINVDFVATIKGTTCNITLE
                     GTRVTADGNNQYTLRILNVALDKIINKMPEAQADFKLVANCDGNIGKITTSLAGNPSV
                     ELPYLISPLTSDNSSTTEYIAMGIKLRNASDTEFIPPDGSKKISWGEGLTKPELEMTV
                     ALRETRPGSGRAGSFKALATFNFTYE"
     gene            complement(1186..3786)
                     /locus_tag="IU325_21685"
     CDS             complement(1186..3786)
                     /locus_tag="IU325_21685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HAP5811602.1"
                     /translation="MKTTTSTKRHNLTRIAMYCSIMYSGATIGAESVEYDPTFLMGGN
                     ASSIDVSRYSDGNPTLPGVYDVSIYVNEQPVANLEIPFIAIPDKKNAQACITLKNLLQ
                     LHIKTPPADEENTILLPRDETLGNCLDLSLAIPKSSVNYDPSEQRLDINVPQAWVMKN
                     YQNYVDPSLWENGINAATLSYNINAYRSENSNYTNDSVYTSFNGGVNLGAWRLRSSGN
                     YSWRNDAGSNVEFMNRYVQRDITAIRSQLIMGESYTTGETFDSVSIRGIRLYSDSRML
                     PPVLANFAPTIRGVANTNAKVSITQSGYKIYETTVPPGPFVIDDLSPSGYGSDLIVTI
                     EEADGSKRTFSQPFSSVIQMLRPGVSRWDISGGQINKDDLHHEPNLLQATYYRGLSNL
                     FTGYTGFQVTDNHYAAGLLGIGMNTSVGAISFDVTHSSVDIPDDKRYQGQSYRISWNK
                     FFDLTDTSLNIAAYRYSTQDYLGLNDALTLIDEVEHPTQDLDPKTMRNYGRMKNQFTV
                     SINQPLRFGKDDYGSFYTSGSWSDYWGGSKSRSNYSVGYSNSASWGSYSISAQRSWDE
                     YSQTENSIYLSFSIPIEKLMGTEHRDSGFQSIDTQLSTNMDGNNQFNMSSSGYSNDNR
                     VSYSVNAGYGMNKTGKDLSNIGGYASYESPWGTLAGSVSATSDNNRQYSVNTDGGFVL
                     HSGGLTFSNDSFSDNDTIALVKAPGAKGARINYGNNTVDRWGYGVTSALSPYQENKIA
                     LDTENLENDIEMKSTSTVAVPRQGSVIFAGFETNQGQSAIMNIKRSDGKGIPFAADIH
                     DETNAIIGNVGQGGQAFVRGIQQQGTIKITWLEASMPKTCIAQYQQPNVTSEKIQQTI
                     ILNNILCQLQ"
     gene            complement(3821..4555)
                     /locus_tag="IU325_21690"
     CDS             complement(3821..4555)
                     /locus_tag="IU325_21690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HAP5811603.1"
                     /translation="MLLNKKNIVALCVASSMAFSMPVMADIVISGTRVIYKSDQKSVS
                     VRLENKGNSHLLVQSWLDTGDDNAEPGSINVPFTATPPVSRIDAKRGQTIKLMYTGST
                     VLPSDKESVFWFNVLEVPPKPDATKVENQSLLQLAFRTRIKLFYRPAGLNGNPSEAPL
                     ALKWKWAEGKSGLSVYNPTPYYVSFNSAELIAGGKSNPLNVKMVAPKSEEIFTISGSG
                     GKATSGKVHFSAINDFGGAIEGDVNL"
     gene            complement(4645..5217)
                     /locus_tag="IU325_21695"
     CDS             complement(4645..5217)
                     /locus_tag="IU325_21695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP5811604.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQINISGLVSDNTCETK
                     VDGGKQDGLVLLQTATVAEITAGVSETAVGAKAKPFSITVDCSKATPGGQPTLTFGSV
                     FFGNSRGTLNNDMSITTPSSGVNIALHNIDGTTVKQVKINNASDAYTKALTNNVAVFD
                     FKASYVREDDSTPATAGYVRTNTAYTITYN"
BASE COUNT         1367 a         1188 c         1194 g         1567 t
ORIGIN      
        1 taataaatat caactaatac attgctggtg acaaatcctg ccccaacggg aagtaacgga
       61 tcaatttttt gcattcgtgt ataaaattgc cacgatgaat tattcatgtt tgcagaggca
      121 actggccaga ctgtgcgaaa ctgccctgtt ggtgacagca cattcactgt gtccgatgac
      181 tgattatttt ggctaaaaat cacgattcct acattttgtg caccgccgac atgattattg
      241 gcgaatatct gttggctacc agtggccatt acaccactga caggtttaaa ttctatatgc
      301 aactggcttg atgttgtgcc agcggagggg caatctttta ctgtcacagt aaaggtcttt
      361 ccgcctggat aattatcttc cgcactaaca ttactggcaa aatatcccac tccaacagtg
      421 ccaagattaa ccaacccctg gttgctgatt tcgctttgac aagttctgtt ttcaacagtg
      481 gtcataacgt ttaggccaat gttatccgtg atggctatag aaaacgtgct gaataataat
      541 aagctcagag taatgcttgc aaatatccca taatgtaatg atttcatctg cattattatt
      601 atccttttat tcgtaggtga aattgaatgt cgcaagtgct ttaaaactac ctgctcttcc
      661 tgaacctggt cgggtttcgc gaagtgctac cgtcatctca agttccggtt ttgttaatcc
      721 ctctccccag gaaatctttt tagaaccatc tgggggaata aattcagtgt cagaggcatt
      781 tcgaagttta atccccatag caatatattc tgtggtggat gaattatctg aagttaatgg
      841 agaaattaaa tagggtaact ctactgaagg attccctgcc agcgatgtcg taatcttgcc
      901 tatgttacca tcacaattag cgaccagttt aaagtcggct tgtgcttctg gcatcttgtt
      961 tatgatttta tccagagcaa catttaatat ccgtagcgtg tactgattat tgccatcagc
     1021 ggtgacgcgt gtcccttcca gagtgatatt acatgtcgtg cctttgattg tggcaacaaa
     1081 atcaacatta atatcttgtg ccagtgcgcc gggcagtaaa aaaatgcctg ccaggagtac
     1141 tattttatac cgtgtgtttt ttatcattgt catctctctt tcgtattatt ggagctggca
     1201 aagaatatta ttgagaataa tcgtttgctg gattttctcc gatgtcacat tgggttgctg
     1261 gtactgcgca atacaggttt tgggcatgct ggcttccagc caggtgattt ttatcgtgcc
     1321 ctgctgctga ataccgcgaa caaatgcctg cccgccctgc ccaacgttgc caatgattgc
     1381 atttgtttca tcatgaatat cggcggcgaa ggggatacct tttccgtcac tgcgtttgat
     1441 attcataatc gcagattgtc cctgattggt ttcaaaacca gcaaagataa ccgagccctg
     1501 gcgtggtacg gcaaccgtac tggtgctctt catttcgatg tcgttttcca ggttttcagt
     1561 gtccagagca attttgtttt cctggtatgg tgaaagagca ctggttacgc catatcccca
     1621 acggtcaacg gtattgttgc cgtagttaat acgcgcccct tttgcacccg gtgctttaac
     1681 cagagcaatg gtgtcattat cactgaaact gtcgttgctg aacgtcagtc ctccgctgtg
     1741 caacacaaat ccaccatctg tattaacgga gtactggcgg ttattatcgc tggtagcaga
     1801 gactgaccca gctagcgttc cccatggcga ttcatagctg gcataacccc caatgttgct
     1861 gagatctttg ccggttttat tcatcccata acccgcgttc acgctatagc tcacgcggtt
     1921 atcgttgcta taaccgctac tgctcatgtt gaactgatta ttaccatcca tattggtact
     1981 aagttgtgta tcaatacttt ggaaaccgga atcacggtgc tccgtaccca tcagcttttc
     2041 gataggtatc gaaaagctga ggtaaatact gttttctgtt tggctgtact catcccagga
     2101 tcgttgagcg ctgatgctgt agcttcccca tgaagcgctg ttactgtagc cgaccgaata
     2161 gttgctacgg ctcttactgc ccccccagta atcagaccag cttccagatg tatagaacga
     2221 gccataatca tccttcccaa agcgtagcgg ttggttaatg cttacggtga actggttttt
     2281 catgcgcccg tagttgcgca tagttttggg atcaagatcc tgagtgggat gttcaacttc
     2341 atcaataagt gttaatgcat cattcaggcc cagatagtcc tgcgtggagt agcggtaagc
     2401 cgcgatgttt aatgatgtat ctgtcaggtc aaaaaatttg ttccaggaga tgcgatagct
     2461 ttgcccctgg taccttttat catccggtat atcgacactg gagtgggtga catcgaagga
     2521 aattgcgccg acagaagtat tcatcccaat gccaagtaag cctgcagcat aatgattatc
     2581 tgtgacctga aaaccagtat agccagtgaa aaggttactt aaaccacggt aataggtcgc
     2641 ttgtagtaag tttggttcat gatgcaagtc gtctttatta atttgcccgc cgctgatatc
     2701 ccagcgagaa acacccgggc gcagcatttg aattactgaa gaaaatggct gagaaaatgt
     2761 acgcttgctg ccatcagctt cttcaatggt tacgataaga tcgctgccat accctgacgg
     2821 gctgagatca tcaatgacaa aaggtcccgg tggaacagtg gtttcgtaaa ttttgtatcc
     2881 actctgggtg atgctgactt tggcgttagt atttgctaca ccacgaattg tcggggcgaa
     2941 gtttgccaat acaggcggca gcattcggct gtcgctgtat aagcgaatgc cacggatact
     3001 aacggagtcg aacgtttcac ctgtggtgta ggattccccc ataatgagct gcgagcgaat
     3061 agcggtaatg tcgcgctgga cgtagcgatt cataaactca acattgcttc ccgcgtcgtt
     3121 tctccagcta tagttaccgc tgctacgcag acgccaggcc cctaaattga cgccaccatt
     3181 gaacgatgta taaacgctat catttgtata atttgaattt tcactgcgat aagcgttgat
     3241 gttataagac agcgtagccg cgttaatgcc attttcccaa agtgatgggt ccacatagtt
     3301 ttgataattc ttcatcaccc atgcctgggg aacattgata tccagacgtt gttcagaggg
     3361 gtcatagttc actgaggatt taggtatggc taacgacagg tcaagacagt tgccaagcgt
     3421 ttcatcccgt ggaagaagga tagtattttc ctcgtctgcc gggggtgtct tgatatgcag
     3481 ttgtagtaga tttttcaggg taatacaggc ttgagcattc tttttatcgg ggatcgcgat
     3541 aaaggggatc tccaggttag caacaggttg ctcgttaaca tagatgctaa catcgtaaac
     3601 accaggcaaa gtcgggttgc catcgctata gcggctaaca tcaatactgg aagcgttgcc
     3661 acccattaaa aaggtagggt catactcaac gctttcggct ccgatggtag cgccgctata
     3721 cataattgag caatacattg cgatacgcgt aagattatgg cgtttagtcg atgtcgttgt
     3781 tttcattaca ttggcttcct gctgcataag gtccctggtc ttacaaattg acatcgcctt
     3841 caattgcgcc gccaaaatca ttaatcgcgg agaaatgtac ctttcctgac gttgctttcc
     3901 cgcctgaacc gcttatggtg aaaatctctt cgcttttcgg tgcaaccatt tttacattga
     3961 gcgggttcga cttaccgcca gcgattaact ctgcactgtt gaaagagacg taataaggtg
     4021 ttgggttata tacactcagg ccagacttcc cttcagccca tttccatttc agggctaatg
     4081 gtgcttcaga cgggttaccg tttaatcctg ccggacgata gaacagcttg atacgggtac
     4141 ggaaagccag ttgtaacagg ctctgattct ctacttttgt agcatctggt tttgggggca
     4201 cttccagcac gttaaaccag aatacgcttt ctttatcgct ggggagtacg gtactgccgg
     4261 tatacatcaa tttgatggtt tgaccgcgtt tagcatcaat acgtgaaacg ggaggtgttg
     4321 ccgtaaatgg tacattaatg cttcctggct cagcgttatc gtcaccggta tccagccagc
     4381 tttgtactaa caggtggcta ttgcctttat tctccaggcg tacgctaacg cttttttgat
     4441 cacttttata aatgacgcgt gtaccggaaa taacaatatc agccataaca ggcatgctaa
     4501 atgccatact actggcaaca cataatgcga caatattctt tttatttaac aaaatatacc
     4561 atccatgaca tgaattgttt tcaagacaag ggggcgacaa taggtgtcgc tcccttgtag
     4621 taatttaaat caccaacgaa taacttaatt gtaggtgatg gtgtaagctg tgttggttct
     4681 tacataacca gccgttgctg gtgttgagtc gtcttcacga acataggagg ctttgaagtc
     4741 aaagactgca acattattgg ttaatgcttt agtgtaagca tcagatgcat tattaatttt
     4801 gacttgttta acagtggtgc cgtcaatgtt gtgcagggca atattcacac cagatgacgg
     4861 ggtggtgatg gacatatcgt tattcaaagt gccacgactg ttaccaaaga aaactgaacc
     4921 gaatgtcaga gtcggttggc ctcccggagt cgctttgctg cagtctacag taatgctaaa
     4981 cggtttcgct ttggcaccga cagcagtttc agatacacca gcggtaattt cagcaaccgt
     5041 tgcagtttgc agaagaacca ggccatcttg cttgccgcca tcaacttttg tttcacaggt
     5101 gttatcgcta accaagccac tgatgttaat ctgaccaccg tccatatcag cagaagcagt
     5161 acctgcaacc attgccagca taagaccaga cagggcaaaa cctaattttt tagacataat
     5221 aatccttgaa ttatattcca tgagtttaac tacaatcggg tgctttcaga caaagaaccc
     5281 cgatagagaa aaatagattg gtttgttaat gatgaa
//
LOCUS       DACBVI010000233         5311 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.235, whole genome shotgun sequence.
ACCESSION   DACBVI010000233 DACBVI010000000
VERSION     DACBVI010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.235"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            165..1499
                     /gene="cadB"
                     /locus_tag="IU325_21700"
     CDS             165..1499
                     /gene="cadB"
                     /locus_tag="IU325_21700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HAP5811605.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            1579..3726
                     /locus_tag="IU325_21705"
     CDS             1579..3726
                     /locus_tag="IU325_21705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HAP5811606.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSNWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            3785..5242
                     /gene="dtpC"
                     /locus_tag="IU325_21710"
     CDS             3785..5242
                     /gene="dtpC"
                     /locus_tag="IU325_21710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HAP5811607.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESH
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFAARFLFSTPTNMIQESND"
BASE COUNT         1253 a         1267 c         1291 g         1500 t
ORIGIN      
        1 aaaacctcaa gttctcactt acagaaactt ttgtgttatt tcacctaatc tttaggatta
       61 atcctttttt cgtgagtaat cttagcgcca gtttggtctg gtcaggaaat agttatacat
      121 catgacccgg actccaaatt caaaaatgaa attaggagaa gagcatgagt tctgccaaga
      181 agatcgggct atttgcctgt accggtgttg ttgccggtaa tatgatgggg agcggtattg
      241 cattattacc tgcgaaccta gcaagtatcg gtggtattgc tatctggggt tggattatct
      301 ctattattgg tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca aaaaacccgc
      361 aacaaggtgg cccaattgct tatgccggag aaatttcccc tgcatttggt tttcagacag
      421 gtgttcttta ttaccatgct aactggattg gtaacctggc gattggtatt accgctgtat
      481 cttatctttc caccttcttc ccagtattaa atgatcctgt tccggcgggt atcgcctgta
      541 ttgctatcgt ctgggtattt acctttgtaa atatgctcgg cggtacctgg gtaagccgtt
      601 taaccactat tggtctggtg ctggttctta ttcctgtggt aatgacagct attgttggct
      661 ggcattggtt tgatgcggca acttatgctg ctaactggaa tactgcggat accactgatg
      721 gtcatgcgat cattaaaagt attctgctct gcctgtgggc cttcgtgggt gttgaatccg
      781 cagctgtaag tactggtatg gttaaaaacc cgaaacgtac cgttccgctg gcaaccatgc
      841 tgggtactgg tttagcaggt attgtttaca tcgctgcgac tcaggtgctt tccggtatgt
      901 atccgtcttc tgtaatggcg gcttccggtg ctccgtttgc aatcagtgct tcaactatcc
      961 tcggtaactg ggctgcgccg ctggtttctg cattcaccgc ctttgcgtgc ctgacttctc
     1021 tgggctcctg gatgatgttg gtaggccagg caggtgtacg tgccgctaac gacggtaact
     1081 tcccgaaagt ttatggtgaa gtcgacagca acggtattcc gaaaaaaggt ctgctgttgg
     1141 ctgcagtgaa aatgactgcc ctgatgatcc tcatcactct gatgaactct gccggtggta
     1201 aagcatctga cctgttcggt gaactgaccg gtatcgcagt actgctgact atgctgccgt
     1261 acttctactc ttgcgttgac ctgattcgtt ttgaaggcgt taacatccgc aactttgtca
     1321 gcctgatctg ctctgtactg ggttgcgtgt tctgcttcat cgcgctgatg ggcgcaagct
     1381 ccttcgagct ggcaggtacc ttcatcgtca gcctgattat cctgatgttc tacgctcgca
     1441 aaatgcatga gcgccagagc cactcaatgg ataaccacac agcgtctaac gcacattaat
     1501 taaaagtatt ttccgaggct cctcctttca ttttgtccca tgtgttggga ggggcctttt
     1561 ttacctggag atatgactat gaacgttatt gcaatattga atcacatggg ggtttatttt
     1621 aaagaagaac ccatccgtga acttcatcgc gcgcttgaac gtctgaactt ccagattgtt
     1681 tacccgaacg accgtgacga cttattaaaa ctgatcgaaa acaatgcgcg tctgtgcggc
     1741 gttatttttg actgggataa atataatctc gagctgtgcg aagaaattag caaaatgaac
     1801 gagaacctgc cgttgtacgc gttcgctaat acgtattcca ctctcgatgt aagcctgaat
     1861 gacctgcgtt tacagattag cttctttgaa tatgcgctgg gtgctgctga agatattgct
     1921 aacaagatca agcagaccac tgacgaatat atcaacacta ttctgcctcc gctgactaaa
     1981 gcactgttta aatatgttcg tgaaggtaaa tatactttct gtactcctgg tcacatgggc
     2041 ggtactgcat tccagaaaag cccggtaggt agcctgttct atgatttctt tggtccgaac
     2101 accatgaaat ctgatatttc catttcagta tctgaactgg gttctctgct ggatcacagt
     2161 ggtccacaca aagaagcaga acagtatatc gctcgcgtat ttaacgcaga ccgcagctac
     2221 atggtgacca acggtacttc cactgcgaac aaaattgttg gtatgtactc tgctccggca
     2281 ggcagcacca ttctgattga ccgtaactgc cacaaatcgc tgacccacct gatgatgatg
     2341 agcgatgtta cgccaatcta tttccgcccg actcgtaacg cttacggtat tcttggtggt
     2401 atcccacaga gtgaattcca acacgctacc attgctaagc gcgtgaaaga aacaccaaac
     2461 gcgacctggc cagtacatgc cgtaattacc aactctacct atgatggtct gctgtacaat
     2521 accgacttca tcaagaaaac actggatgtg aaatccatcc actttgactc cgcgtgggtg
     2581 ccttacacca acttctcacc gatttacgaa ggtaaatgcg gtatgagcgg tggtcgtgta
     2641 gaagggaaag tgatttacga aacccagtct actcacaaac tgctggcggc gttctctcag
     2701 gcttccatga tccacgttaa aggtgacgta aacgaagaaa cctttaacga agcctacatg
     2761 atgcacacca ccacttctcc gcactacggt atcgtggcgt ccactgaaac cgctgcggcg
     2821 atgatgaaag gtaatgctgg taagcgtctg atcaacggtt ccattgaacg tgcgatcaaa
     2881 ttccgtaaag agatcaaacg tctgagaacg gaatctgatg gctggttctt tgatgtatgg
     2941 cagccggatc atatcgatac gactgaatgc tggccgctgc gttctgacag caactggcac
     3001 ggcttcaaaa acatcgataa cgagcacatg tatcttgacc cgatcaaagt caccctgctg
     3061 actccgggga tggaaaaaga cggcaccatg agcgacttcg gtattccggc cagcatcgtg
     3121 gcgaaatacc tcgacgaaca tggcatcgtt gttgagaaaa ccggtccgta taacctgctg
     3181 ttcctgttca gcatcggtat cgataagacc aaagcactga gcctgctgcg tgctctgaca
     3241 gacttcaaac gtgcgttcga tctgaacctg cgtgtgaaaa acatgctgcc gtctctgtat
     3301 cgtgaagatc ctgaattcta tgaaaacatg cgtattcagg aactggctca gaatatccac
     3361 aaactgattg ttcaccacaa tctgccggat ctgatgtatc gcgcatttga agttctgccg
     3421 accatggtaa tgactccgta tgctgcgttc cagaaagagc tgcacggtat gaccgaagaa
     3481 gtttacctcg acgaaatggt aggtcgtatt aacgccaata tgatccttcc gtatccgccg
     3541 ggagttcctc tggtaatgcc gggtgaaatg atcaccgaag aaagccgtcc ggttctggag
     3601 ttcctgcaga tgctgtgtga aatcggcgct cactatccgg gctttgaaac cgatattcac
     3661 ggtgcatacc gtcaggctga tggccgctat accgttaagg tattgaaaga agaaagcaaa
     3721 aaataattag ctcgtacaag ggaagtggct tgccacttcc cttttttgct cataaggaga
     3781 acacatgaaa acaccctcac agccgcgcgc gatatactat atcgtggcga tccaaatctg
     3841 ggagtacttc agtttttacg gcatgcgtgc cttactcatt ctctatctca cccatcagct
     3901 tggttttgat gataaccatg ccatcagcct gttcagcgca tatgcttctc tggtttacgt
     3961 tacccctatt ctcggcggct ggcttgccga ccgcctgctc ggcaaccgca ctgcagtgat
     4021 tgccggcgcg ctgttaatga cccttggcca tgtggtgctg ggcattgata caaattcaac
     4081 ttttagcctg tatctggcgc tggcaatcat tatttgtggc tacggtttat tcaaatcaaa
     4141 catcagctgt ttgcttggcg agctctacga cgagaacgat catcgacgtg atggcggttt
     4201 ttcgctgctg tatgctgcgg gcaatatcgg ttctatcgcc gctcccatcg cctgcggcct
     4261 ggctgctcag tggtatggat ggcatgttgg ctttgccctt gcgggtggcg gcatgtttat
     4321 cggtttgttg attttcttaa gtggtcatcg tcatttccag tccacgcgta gtatggataa
     4381 aaaagcgctc actagcgtta aatttgcctt accagtatgg agctggttag tggtgatgct
     4441 ctgtttagcc cctgtatttt ttaccctgct gctggagaac gactggtcag gatatttgct
     4501 ggcgatcgtt tgcctcattg ccgcacaaat cattgcccgc atgatgatca aattccccga
     4561 acaccgccgt gctctttggc aaattgtatt gttgatgttt gtcgggacat tgttctgggt
     4621 actggcacaa cagggcggca gtaccatcag cttgtttatc gatcgctttg tgaatcgtca
     4681 ggcattcaat attgaagtac ctactgcact attccagtca gtgaacgcaa ttgcggtgat
     4741 gctcgctggg gttgttctgg cctggctggc gtcgccagaa agccacggca actcaacatt
     4801 gcgcgtctgg ctgaagtttg cctttggctt attgctgatg gcttgtggct ttatgttgct
     4861 ggcatttgat gcccgacatg cagcggctga cggtcaagcg tcaatgggcg tgatgatatc
     4921 cgggctggcg ctaatgggct ttgccgaact ctttattgac ccggtggcga ttgcgcaaat
     4981 cacgcgtctg aaaatgtctg gcgtattaac cggtatttat atgctggcaa caggcgcggt
     5041 cgccaactgg ctggcaggcg tcgtggcaca gcagaccaca gagtcgcaaa ttagcggtat
     5101 ggcaattgcc gcttaccagc gattcttttc tcaaatggga gagtggacgt tggcttgtgt
     5161 cgcgatcatc gtcgtattgg cctttgctgc ccgttttctg tttagcacgc caacgaatat
     5221 gatacaggag agtaacgatt aactgtcgta aaaacaatta gttatgaata aattaacttt
     5281 cagtttatca cactaataaa tcgagattaa t
//
LOCUS       DACBVI010000234         5230 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.237, whole genome shotgun sequence.
ACCESSION   DACBVI010000234 DACBVI010000000
VERSION     DACBVI010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.237"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            81..4292
                     /locus_tag="IU325_21715"
     CDS             81..4292
                     /locus_tag="IU325_21715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HAP5811608.1"
                     /translation="MSGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPNSPV
                     EEQKGNPVNPLLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAP
                     FDIRLQVHERELILNDSGGRSIHFEPLFPGEISYSRSESFWLARGGVAEQHSSQPLSA
                     LWQVLPESLRLSPHVYLATNSLQGPWWILGWPERVPEADEVLPPEPPAYRVLTGVVDG
                     FGRTLAFHRAAEGEAAGAVTGVTDGAGRRFHLALTTQAQRAKAFRKQRESSLSSPASP
                     RSVSSSQFFPDTLPTGTEYGADNGIRLEAVWLTHDPAYPDELPTAPLARYTYTASGEL
                     RAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTEQVNPEGLDYRF
                     EYGQDRVTITDSLNRREVLYTEGEGGLKRVVKKEYGDGSITRSEYDEAGRLKAQTDAA
                     GRRTEYRLHMASGKLTSVILPDGRTVRYGYNNQLQLTSVTYPDGLRRSRKYDRQGRLA
                     EEVSRNGNITRWFYDSSRSGLPCAVEDGTGVRRRITRNRYGQLQAFTDCSGYTTRYEY
                     DRYGQRTAVHREEGISTYSSYNPRGQLVSQKDAQGRETRYEYSAAGDLTAIIAPNGSR
                     SETQYDAWGKAVSTTQGGLTRSMEYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGF
                     DGRTQRYRYSATGQLIRSEDEGQVTQWYYDDAGRITHRTVNGDPAEQWQYDDHGWLTE
                     ISHLSEGHRVAVHYGYDDKGRLTGERQTVENQETGEMLWEHETRHVYSEQGLATRQEP
                     DGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGAGSTAGYEQATAYT
                     LTGQLRSRHLNLPQLDREYTWNDNGQLVRISGPQESRKYRYSDTGRLTGVHTTAANLD
                     IDIPYATDPAGNRLPDPELHPDSTFTAWPDNRITEDAHYVYRYDEYGRLAEKTDRIPE
                     GVIRMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDL
                     TGWMSLSRKPEETWYGWDGDRLTTVQTGTTRIQTVYQPGSFTPLIRIETENGEQAKAR
                     HRSLAEVLQEDTGVTLPAELSVMLGRLERELRAGAVSAESEAWLQQCGLTAEQMKNQM
                     EAEYIPERTLHLYHCDHRGLPQALISPEGETAWQGEYDEWGNLLGETSAQQLQQPYRL
                     PGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGAWNLYQYPLNPTTKIDPLGLSG
                     EDSIEQSGPSWLIADPMDKYRQQAVMGLTKLWDDTFSQEKTKCLPTTPENIRMVVANS
                     PLLTVQQKVSVPVVAHYVQMLEDGSGAPPVDMDGNVIVNGNHRMVAGLLCDKLPPERP
                     GTRPLSAPLYPFSQIFPELDDWGNR"
     gene            4289..4774
                     /locus_tag="IU325_21720"
     CDS             4289..4774
                     /locus_tag="IU325_21720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP5811609.1"
                     /translation="MIRIAFLITNKFLDIDSTTLAGYDFSGMDLHRAILNGYCLDGAK
                     FENSHLRNVMIQHTSTRNAVFRNAALMNAILNNSDFTGSDFSNSRSIGANFEGAILTN
                     STFCNADVKDSNFSGADLCGANLLCDRYGDINITGALFDKNTSWLEGFDPIAHGAILK
                     I"
BASE COUNT         1235 a         1305 c         1707 g          983 t
ORIGIN      
        1 cggggaaatc ttattcactg gttatgaatc agtatggatt gcactcagaa taaaaccata
       61 aataatttaa ggaataaacc atgagcggaa aaccggcggc gcgacagggc gatatgaccc
      121 gcaagggact ggatattgtg cagggttcag cgggggtgct gataggtgcg ccgacgggag
      181 tggcctgctc ggtgtgtccc aacagcccgg tggaggagca gaagggtaac ccggtgaacc
      241 cgctactggg tgcgaaggtg ctgccgggcg agacggatct tgcgctgccc ggcccgttgc
      301 cgtttattct ttcccgcgcc tacagcagct accggaccag gacacccgcg ccggtggggg
      361 tgtttggtcc cggctggaaa gcgcccttcg atatccgctt acaggttcat gagcgggaac
      421 tgatactcaa cgacagtggc ggcaggagca ttcactttga gccgctgttt cccggcgaga
      481 taagctacag ccgcagcgag tcgttctggc tggcaagggg cggggtggcg gagcagcaca
      541 gttcgcagcc gctaagcgcg ctctggcagg tgctgccgga gagtctccgc ttaagcccgc
      601 atgtgtacct ggcgacaaac agccttcagg ggccgtggtg gatactcggc tggccggagc
      661 gggtgccgga ggcggacgag gtgctgccgc cggagccgcc cgcataccgg gtgctgacgg
      721 gcgtggtgga cggcttcggg cggacgctgg ccttccaccg ggcggcagag ggggaagcgg
      781 cgggcgcggt gacgggggtg acggacggcg cgggccgccg ttttcatctg gcgctgacca
      841 cacaggcgca gcgggcgaaa gccttccgta aacagcgcga gtcttcttta tcttctccgg
      901 ccagtccccg ttctgtttcc tcttctcagt ttttccccga cacgctgccc accggtacag
      961 agtacggtgc cgataacggt atccggctgg aggcggtatg gctgacacac gacccggcat
     1021 acccggatga actgccgact gcgccgctgg cgcgctacac gtacacggcc agcggagaac
     1081 tgcgggcggt gtatgaccgc agcgggacgc aggtgcgcgg gtttacttat gatgcggagc
     1141 acgccgggcg gatggtggcg caccattatg cggggaggcc ggagagccgc taccggtatg
     1201 atgataccgg tcgggtgacg gagcaggtca acccggaggg gctggactac cgctttgagt
     1261 acgggcagga ccgcgtgacc atcacggaca gcctgaaccg gcgggaggtg ctgtacacgg
     1321 agggcgaggg cggcctgaag cgggtggtga agaaggagta tggggacggg agcatcaccc
     1381 gcagcgagta tgatgaggcg gggaggctga aggcgcagac ggatgcggcg ggacggcgga
     1441 cggagtaccg gctgcatatg gcgtcgggaa agctgacttc agtgatactg ccggacggca
     1501 ggacggtacg gtatggttac aacaaccagt tgcaactgac gtcagtgacg tacccggacg
     1561 ggctgcgccg cagccggaaa tatgaccgtc agggcaggct ggcggaagag gtctcgcgca
     1621 acggtaacat tacgcgctgg ttttatgatt cttcccgcag tggcctgccc tgtgcggtgg
     1681 aagacggaac cggggtgagg cggagaataa cgcgaaaccg gtacggtcag cttcaggcat
     1741 ttacggactg ttcagggtac acgacgcggt atgaatacga ccggtacggt cagaggacgg
     1801 cggttcaccg ggaggaaggc atcagcactt acagcagtta taacccgcgt ggtcaactgg
     1861 tcagtcagaa ggatgcgcag ggacgtgaaa cccgttatga gtacagcgcc gcaggcgacc
     1921 tcaccgctat cattgccccg aacggcagcc ggagtgagac acagtatgat gcgtggggaa
     1981 aggccgtcag caccacgcag ggcggactga cgcgcagcat ggagtatgac gccgccgggc
     2041 gcatcaccgt gctgaccaac gagaacggca gccagtccac gttccggtat gacccggtgg
     2101 acaggctgac ggagcagcgc ggctttgacg gcaggacaca gcgttaccgg tacagcgcca
     2161 cagggcagct catccgcagc gaggatgagg ggcaggtcac gcagtggtac tacgatgacg
     2221 ccgggcgcat cacgcaccgg acggtgaacg gcgacccggc ggagcagtgg cagtatgacg
     2281 accacggctg gctgacagaa atcagccacc tgagcgaagg ccaccgtgtg gcggtccact
     2341 acggctatga cgataaaggc cgcctgacgg gcgaacggca gacggtggag aaccaggaga
     2401 cgggggagat gctgtgggag catgagacga ggcacgtcta cagcgaacag gggctggcga
     2461 cccgtcagga gccggacggt ctgccgccgg tggagtggct gacgtatggc agcggttatc
     2521 ttgcggggat gaagctgggc ggaacgccgc tggtcgagta cacgcgggac cggctgcacc
     2581 gtgagacggc ccgcagcttc ggcggggcgg gcagtacagc ggggtacgaa caggccacgg
     2641 cgtacaccct cacagggcag ctccggagca ggcacctgaa ccttccgcag cttgaccgtg
     2701 agtatacgtg gaacgataac gggcaactgg tgcgcatcag cggcccgcag gagagccgga
     2761 agtaccgtta cagcgacacg ggaagactga cgggcgttca caccacggcg gcgaacctgg
     2821 atatcgatat cccgtatgcg acggacccgg caggaaaccg gctgccggac ccggaactgc
     2881 acccggacag caccttcacg gcgtggccgg ataaccgcat cacggaagat gcgcactatg
     2941 tctaccgcta cgatgaatac ggcaggctgg cggagaagac ggaccgtatc ccggaagggg
     3001 tgatacggat gcacgacgag cggacgcacc actaccatta cgacagccag caccgcctgg
     3061 tgttctacac gcggatacag cacggcgagc cacaggtgga gagccgctac ctctatgacc
     3121 cgctgggccg ccggacgggg aaacgggtgt ggcggcggga gcgtgacctg acggggtgga
     3181 tgtcgctgtc gcgtaaaccg gaggagacct ggtacgggtg ggacggtgac aggctgacga
     3241 cggtacagac cggcaccacg cgtatccaga cggtatacca gccggggagc ttcacgccgc
     3301 tcatcagaat cgaaacagag aatggtgaac aggcgaaggc gcggcaccgg agtctggcgg
     3361 aggtgttgca ggaggacacg ggggtgacgc taccggcgga gctgtcggtg atgctgggga
     3421 ggctggagcg ggaactgcgc gcgggtgcgg tgagtgcgga gagtgaggca tggcttcagc
     3481 agtgcgggct gacggcggaa cagatgaaaa accagatgga ggcggaatac atcccggaga
     3541 ggacacttca tctttaccac tgcgaccacc gggggctgcc gcaggcgctc atcagcccgg
     3601 aaggggaaac ggcgtggcag ggggagtatg acgagtgggg aaacctgctg ggcgaaacca
     3661 gcgcgcagca actgcaacag ccgtaccgtc tgccggggca gcagtatgat gaggagtcgg
     3721 ggctgtacta taaccgcaac cggtactatg acccgttgca ggggcggtat atcacccagg
     3781 atcccattgg gctaagaggt gcatggaacc tgtatcagta tccattgaat ccaactacta
     3841 agatagatcc gttgggtctc tccggagaag attccataga gcagtcaggt ccatcttggt
     3901 tgattgctga ccccatggat aaatacagac aacaggcagt gatgggttta acaaaattgt
     3961 gggatgatac ctttagtcaa gagaagacta aatgtttacc tacgacacct gaaaacatca
     4021 ggatggttgt ggccaacagt cccttgttga cagtacaaca aaaagtctca gtacccgtag
     4081 tagctcacta cgtacaaatg ctggaagatg gtagtggagc accaccggtt gatatggatg
     4141 ggaatgtgat tgttaatggt aatcacagga tggttgcggg tctgttatgt gataaacttc
     4201 ctcctgagcg gccaggtacg cgtccattat cggcaccact atatccgttt agccaaattt
     4261 ttccggagtt agatgattgg gggaatagat gataagaatc gcgtttttaa taaccaataa
     4321 atttcttgat atagatagta ccactcttgc tggatatgat tttagcggga tggatctaca
     4381 tcgtgccatc ctcaatggtt attgtcttga tggggcgaaa tttgaaaatt cccatttaag
     4441 aaatgtaatg attcaacata catcaactag aaatgctgtc tttcgcaatg cggctctgat
     4501 gaacgctatt cttaataact cagatttcac tggaagtgac tttagtaatt caaggtcaat
     4561 aggtgcaaac tttgagggtg ctatattaac taattcaacc ttttgcaatg cagacgttaa
     4621 agattcaaat ttttctggtg ctgacttatg tggcgctaat ttattatgtg atagatatgg
     4681 cgatataaat ataacaggtg cattattcga taaaaacacc tcatggttag aaggttttga
     4741 tcctatagct catggggcta tattaaaaat atgacaagga tcataactca ttattttctg
     4801 tgtcagtaac actgatgtgg aaagtagact tacttgcaat agccaccggg atctgctgtt
     4861 gaaagccgat cccaaacaac cccggttgct gtggtaaaga gtcatagtag cgagcgcagg
     4921 gggagtgctc agattatatg agatacagtg aaatgaataa acccgaacct gtgttgacgt
     4981 gtcgttagtc ctaagcggca tcagaaccgg aggtttcgtg ataccgccgt gataaattta
     5041 cgggtgaatt ccgaatgcta cgtaaagggt gtccggcgta tagcagacat cagggtaaaa
     5101 gtatgaaagc ccacgcccca caaggcttta ccggatgatg agtttttaaa ctacgacatt
     5161 acttttacag taaaatccat catgttggta ttttctctcg aacaatttga cgatccacgg
     5221 aaagatcacg
//
LOCUS       DACBVI010000235         5147 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.238, whole genome shotgun sequence.
ACCESSION   DACBVI010000235 DACBVI010000000
VERSION     DACBVI010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.238"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            71..1405
                     /locus_tag="IU325_21725"
     CDS             71..1405
                     /locus_tag="IU325_21725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5811610.1"
                     /translation="MILDTVDVKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1421..2191
                     /gene="garL"
                     /locus_tag="IU325_21730"
     CDS             1421..2191
                     /gene="garL"
                     /locus_tag="IU325_21730"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HAP5811611.1"
                     /translation="MNNNVFPNKFKAALAAKQVQIGCWSALSNPISTEVLDLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2221..3111
                     /locus_tag="IU325_21735"
     CDS             2221..3111
                     /locus_tag="IU325_21735"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HAP5811612.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIAEVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3208..4353
                     /gene="garK"
                     /locus_tag="IU325_21740"
     CDS             3208..4353
                     /gene="garK"
                     /locus_tag="IU325_21740"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HAP5811613.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTNDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNIYRASRNIAATLAIGMRNVG"
     gene            4386..4762
                     /gene="rnpB"
                     /locus_tag="IU325_21745"
     ncRNA           4386..4762
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IU325_21745"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1231 a         1212 c         1443 g         1261 t
ORIGIN      
        1 taaataaaac tcataaataa caccttcccc ataataataa aaatcagcat aagtacccga
       61 ggtaaataaa atgattctgg acaccgttga cgtaaaaaag aaaggcgtgc atacccgcta
      121 tttaatatta ctgattattt ttattgttac cgccgttaac tacgccgatc gtgcaacgct
      181 gtctattgct ggtaccgaag tggcaaaaga gttgcagtta agtgcggttt cgatgggtta
      241 catcttctcc gcttttggct gggcctacct gctgatgcaa atccccggcg gctggttgct
      301 tgataagttt ggctcgaaaa aagtttacac ctacagcctc tttttctggt cgctgttcac
      361 tttcctgcaa ggctttgttg atatgttccc gctggcctgg gcagggatct ccatgttctt
      421 tatgcgcttt atgctcggct tctcggaagc gccatcattc ccggcgaacg cccgtattgt
      481 cgccgcctgg tttccgacaa aagaacgtgg tactgcctcc gccatcttta actcggcgca
      541 atatttctcg ctggcgctct tttcgccgct gcttggctgg ctgactttcg cctggggctg
      601 ggagcacgtc ttcaccgtta tgggggtgat tggttttgtg ctgacggcgc tgtggatcaa
      661 gttgattcat aacccgacag atcacccacg tatgtctgcg gaagagctga agtttatctc
      721 agaaaatggc gcggtggtcg atatggacca caaaaagccg ggcagtgcgg cagcaagcgg
      781 tcccaaactg cattacatca agcaattgct ctctaaccgc atgatgctgg gcgtattttt
      841 cggacaatat tttatcaaca ccatcacctg gttcttcctc acctggttcc caatttatct
      901 ggtgcaggaa aaaggcatgt cgattctgaa agtgggtctg gtcgcctcga ttccggcact
      961 gtgtggcttt gcgggcggcg tgctgggagg tgtcttctcg gattatctga tcaaacgcgg
     1021 tttgtccctg accctggcac gtaagctacc gattgtgctg ggaatgttgc tggcttccac
     1081 catcatctta tgtaactaca ccaacaacac cacgctggtg gtcatgctga tggcgctggc
     1141 tttctttggc aaaggatttg gtgcgctggg ctggccagtg atttctgaca ccgcgccgaa
     1201 agagattgtt ggtctctgcg gcggcgtctt taacgtcttt ggcaatgttg cctccattgt
     1261 cactccactg gtgattggct atctggtaag tgaactgcac tccttcaatg ctgcactggt
     1321 tttcgtggga tgttcagcgc tgatggcgat ggtctgctac ctcttcgtag ttggcgacat
     1381 taaacgtatg gaattgcaga aataagcaaa ggtataagcg atgaataaca atgttttccc
     1441 gaataaattc aaagccgcac tggctgcgaa acaggtacaa attggttgct ggtcagcact
     1501 ctctaacccg attagcactg aagttcttga tttggctggg tttgactggc tggtgctgga
     1561 tggcgaacat gcgccaaacg atatctccac gtttattccg cagttaatgg ccctgaaagg
     1621 cagcgccagc gcgccagtag tgcgagtgcc gaccaacgag ccggtaatta ttaagcgtct
     1681 tctggatatc ggtttttata acttcctgat tccttttgta gaaacaaaag aggaagcaga
     1741 gcaggcggtg gcatcaaccc gttacccacc ggaaggcatt cgcggcgtct ccgtttctca
     1801 ccgcgccaat atgtttggca ccgtggcgga ttatttcgct cagtcgaaca agaacatcac
     1861 cattctggtc cagatagaaa gtcagcaggg cgtagataac gtcgatgcca ttgccgctac
     1921 cgaaggcgta gacggtatct tcgtcggccc cagcgatctg gctgcggcat taggccatct
     1981 cggcaatgcc tcgcacccgg atgtacaaaa agcaatccag catattttta accgtgccag
     2041 tgcgcacggc aaacccagcg gtatcctcgc accggtcgaa gccgatgcgc gtcgttatct
     2101 ggaatggggc gcgacgtttg tggctgtcgg cagcgatctc ggcgtcttcc gctctgccac
     2161 tcagaaactg gctgatacct ttaaaaaata accaccaccg caacaagaga gatgattgat
     2221 atgactatga aagttggttt tattggcctg gggattatgg gtaaaccaat gagtaaaaac
     2281 cttctgaaag caggttactc gctggtggtt gctgaccgta acccagaagc tattgctgaa
     2341 gtgattgctg caggtgcaga aacagcttct acggctaaag cgatcgctga acagtgcgac
     2401 gtcatcataa ccatgctgcc aaactcccct catgtgaaag aggtggcgct gggtgagaat
     2461 ggcattattg aaggcgcgaa gccaggtacg gtattgatcg atatgagttc tatcgcaccg
     2521 ctggcaagcc gtgaaatcag cgaagcgctg aaagcgaaag gcattgatat gctggatgct
     2581 ccggtgagcg gcggtgaacc gaaagctatc gacggtacgc tgtcagtgat ggtgggcggc
     2641 gacaaggcta ttttcgacaa atactatgat ttgatgaaag caatggcggg ttccgtggtg
     2701 cataccgggg aaattggtgc aggtaacgtt accaaactgg caaatcaggt cattgtggcg
     2761 ctgaatattg ccgcgatgtc agaagcgtta acgctggcaa ccaaagcggg cgttaacccg
     2821 gatctggtgt atcaggcaat tcgcggtggg ctggcgggca gtaccgtgct ggatgccaaa
     2881 gcgccgatgg tgatggaccg caacttcaag ccgggcttcc gtattgatct gcatattaag
     2941 gatctggcaa atgcgctgga tacttcccac ggcgtcggcg cgcaactgcc gctcacagct
     3001 gcggttatgg agatgatgca ggcactgcga gcagatggtt taggaacggc agatcatagc
     3061 gccctggcgt gctactacga aaaactggcg aaagtcgaag ttactcgtta atgacgtcgt
     3121 gcccggatgg cgtattgcaa tccgggcctg gaatccaggc cgaataagag aaacgccctc
     3181 cggcgtcatg tggtaacagg cataggtatg aaaatcgtaa tcgccccaga ctcttataaa
     3241 gaaagtttat ctgccagcga ggttgcgcag gcgatagaaa aaggatttcg ggaaattttt
     3301 cctgatgcac agtacgtttc tgttcctgtt gccgacggtg gcgaaggaac ggtggaagcg
     3361 atgattgcag ccacccaggg ttccgaacgt cacgcctggg ttacagggcc gctgggcgag
     3421 aaagtgaatg ccagttgggg gatctccggc gatggcaaaa ccgcgtttat tgaaatggcg
     3481 gcggccagtg ggctggagct ggtacctgcg gaaaaacgtg atccactcgt gaccacttca
     3541 cgcggcacag gcgagttaat tctgcaggcg ctggagagcg gcgcgacaaa cattattatc
     3601 ggcattggcg gtagcgctac aaatgatggc ggtgcaggca tggtacaggc gctgggggcg
     3661 aaattatgcg acgccaacgg caatgaaatt ggttttggcg gcggtagtct taatactctg
     3721 aatgatattg atatttccgg cctcgatccg cgcttaaaag attgcgtcat tcgcgtcgct
     3781 tgtgatgtca ccaatccgct ggtgggcgat aacggcgcat cgcgcatctt tggcccacaa
     3841 aagggagcca gtgaagcgat gattgttgag ctggacaata acctctctca ctatgccgat
     3901 gtcattaaaa aagcgctgca tgttgatgtg aaagatgtcc ccggcgcagg agctgcggga
     3961 ggtatgggcg cggcgctgat ggcgtttctt ggtgcggaac tgaaaagtgg tattgaaatc
     4021 gtcacgacgg cgctgaatct ggaggaacat attcacgatt gtacgctggt gatcaccggt
     4081 gaagggcgta ttgacagcca gagtattcac ggcaaggttc cgattggcgt cgcaaacgtg
     4141 gcgaaaaagt accataaacc ggtgattggc attgcgggta gcctgaccaa tgatgttggc
     4201 gttgtacatc agcatggcat tgatgcggtc ttcagcgtat tgaccagcat tggtacgttg
     4261 gacgaagcat tccgcggggc ttatgacaat atttaccgtg cttcacgtaa tatcgccgcg
     4321 acactggcta ttggaatgcg caacgtgggg tgacaagggc gcgcaaaccc tctatactgc
     4381 gcgccgaagc tgaccagaca gtcgccgctt cgtcgtcgtc ctcttcgggg gagacgggcg
     4441 gaggggagga aagtccgggc tccatagggc agggtgccag gtaacgcctg ggggggaaac
     4501 ccacgaccag tgcaacagag agcaaaccgc cgatggcccg cgcaagcggg atcaggtaag
     4561 ggtgaaaggg tgcggtaaga gcgcaccgcg cggctggtaa cagtccgtgg cacggtaaac
     4621 tccacccgga gcaaggccaa ataggggttc ataaggtacg gcccgtactg aacccgggta
     4681 ggctgcttga gccagtgagc gattgctggc ctagatgaat gactgtccac gacagaaccc
     4741 ggcttatcgg tcagtttcac ctgatttacg taaaaacccg cttcggcggg tttttgcttt
     4801 tggaggggca gaaagatgaa tgactgtcca cgacactata cccaaaagaa agcggcttat
     4861 cggtcagttt cacctggttt aacgaaaacc cgccagcgta aactggcggg tttttgcttt
     4921 ttggagcata gtgtaagatg aataaatatc tacggtatta ataatagact ttgtaagaaa
     4981 ttattaagtc tatattgcat ttaaaaggtc gtaatcatac gtatagtcct ctctggtaaa
     5041 atattgcttc tctgaattat atgcttcata tgtgttatat tttacaacct caaagcaatt
     5101 gtcaggagaa ttttcgtctt tctcgagtgt attatctgaa aggaatt
//
LOCUS       DACBVI010000236         5021 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.239, whole genome shotgun sequence.
ACCESSION   DACBVI010000236 DACBVI010000000
VERSION     DACBVI010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.239"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            132..2999
                     /locus_tag="IU325_21750"
     CDS             132..2999
                     /locus_tag="IU325_21750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAP5811614.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKYGAIGLNAIIFSPLL
                     IAADLGSQNGTTITISDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAVANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSSGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSFWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HTSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTIPEPT
                     PNPEPTPAPAQPPIVNPDPTPNPTPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IGSTHASPDYSGGNGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNNSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(3099..5018)
                     /gene="dhaR"
                     /locus_tag="IU325_21755"
     CDS             complement(3099..5018)
                     /gene="dhaR"
                     /locus_tag="IU325_21755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HAP5811615.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSTLGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGHLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQEDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLHSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
BASE COUNT         1327 a         1273 c         1222 g         1199 t
ORIGIN      
        1 gaattattct cagagcagct catcatgatt gcaccataca aatatcatga tgataagagg
       61 atattattca ttgacttaaa caaaatgaca aaatccatag acccatagcc tgggtactat
      121 ataggagtgg tatgggcatc aaacaacaca atgggaatac caaagccgat cgtctcgctg
      181 aattaaaaat ccgttcgccc tccattcaac tgataaaata tggcgctatt ggtttgaatg
      241 caattatctt ttcccccctg ctgatagctg ctgatttagg aagtcaaaat ggcaccacta
      301 ttactattag tgatggtgac agaattacag gagataccgc cgatccatca ggaaacctct
      361 atggtgtaat gaccccagca ggaaacacgc ctggcaatat caacctgggt aatgatgtca
      421 ccgtcaatgt caacgacgcc tctggatatg caaagggaat cattattcag ggcaagaaca
      481 gctccctgac agctaaccga ctcacagtag atgttgttgg tcaaacctct gccatcggca
      541 ttaatttaat tggtgactat acccatgctg acttaggcac aggcagcacc attaagagta
      601 acgatgacgg catcattatt ggacatagct caacactaac agccactcaa ttcaccattg
      661 aaaactcgaa cggtataggc ctaaccatca atgactatgg caccagtgtc gatcttggaa
      721 gcggaagtaa aatcaagacc gatggaagta caggtgttta tatcggtggt ctcaacggca
      781 ataacgccaa tggtgctgcg cgttttacgg cgacagacct gacaatcgat gttcagggct
      841 acagcgccat ggggataaac gtacagaaaa actctgttgt cgatctcgga acaaacagta
      901 ccattaaaac caatggcgat aatgcacacg gcctctggag ctttggccag gtgagcgcga
      961 atgcactcac tgttgatgta actggagccg tggcaaatgg cgtcgaagtt cgtggtggta
     1021 caaccactat cggtgctgat agccatattt cttccgcgca gggcggtggc ctcgtaacca
     1081 gtggttcaga cgcgacaatc aatttttctg gcacggcagc gcaacgaaac agcatctttt
     1141 ccagcggttc ttatggtgcc tcagcccaga cggcaacggc tgttatcaac atgcaaaata
     1201 ccgatattac ggttgatcgt aatggcagtc tggcgctggg tttgtgggcg ctcagcggcg
     1261 gtagaataac cggagacagt ttggctatca ccggcgcggc aggagccagg gggatttatg
     1321 ccatgaccaa cagccagatc gatctcacga gcgatctggt cattgatatg agtacacccg
     1381 accagatggc catcgcaacg caacatgacg atggttatgc cgccagccgc atcaacgctt
     1441 cgggtcgtat gcttatcaac ggtagcgttc tctccaaagg tgggctaatc aatctggata
     1501 tgcaccctgg gtcgttttgg acaggttctt ccctcagcga taatgtcaat ggcgggaaac
     1561 tggacgttgc aatgaataac agcgtctgga acgtaacaag taattctaat ctcgacacgc
     1621 tggcgctgag ccattcaact gtcgattttg ccagccacac gtcaactgcc ggcacattta
     1681 ccacattaaa cgtagagaac ctgagcggta acagtacctt tattatgcgt gctgatgttg
     1741 ttggcgaggg taatggcgtt aataataaag gggatttatt gaatatcagc gggagtagtg
     1801 ctggtaatca cgtattggct atccgtaacc agggcagcga ggccacaacg ggaaatgaag
     1861 ttctgacagt ggtaaaaacc actgacggcg cggcctcgtt cagcgcgtct tctcaggttg
     1921 agttgggagg atatctgtat gatgtgcgta aaaatggcac taactgggag ctttatgctt
     1981 ccgggacaat tccggagccg actcctaatc ctgagcccac accagctccc gctcagcccc
     2041 ccatagtcaa ccccgatcct acgcctaacc ccactcccac acctaaaccc accacgaccg
     2101 cagatgctgg cggcaattat ctcaatgtcg gttacttatt gaactatgtt gaaaaccgta
     2161 cgctgatgca acggatgggt gacctgcgaa atcagagtaa agacggaaat atctggctgc
     2221 gcagttatgg gggaagtctg gactccttcg ccagtggcaa actgagcggc tttgacatgg
     2281 gttacagcgg tatccagttt ggtggggata aacgtctctc tgatgtaatg ccgttgtatg
     2341 tcggtctgta tattggctca acacatgcat cgccggacta tagcggaggc aacggtaccg
     2401 cacgttcaga ctacatggga atgtacgcca gttacatggc acaaaacggt ttttacagcg
     2461 atctcgttat aaaagcatcg cgccagaaaa atagtttcca cgtactggac agtcagaaca
     2521 acggcgttaa cgccaacggc actgcgaatg gaatgagtat ctccctggaa gccgggcaga
     2581 ggttcaacct gtcccctact ggttatgggt tctatataga gccgcaaacc cagctcacat
     2641 acagccacca gaatgagatg gctatgaagg cgagtaatgg cctcaatata catctgaatc
     2701 actacgaatc gctgctgggg cgtgccagca tgatactggg atatgacatc accgcaggca
     2761 acagccagct gaatgtctat gtgaagactg gcgctatccg cgagttttca ggggataccg
     2821 aatatctgtt gaacaactcc cgggagaagt acagtttcaa aggtaatggc tggaataacg
     2881 gcgtgggagt cagtgcacag tataacaaac agcacacatt ctatctcgaa gcggattaca
     2941 cgcagggtaa cctctttgat cagaagcaag tcaacggagg atatcgcttc agcttttaag
     3001 tgggggatag acaagccctt aaacgatgct gaaaattgtt agcgtgcgcc ggaatgaaat
     3061 acagatcact ctgatgtatc ataaatcgtt tctgtctttc atacccggcg cttaaactgc
     3121 cctgcatcaa tgccatgttg cttcattttc cgccacagcg tagtgcggcc gattccaagt
     3181 aaagccgaca tttcctgaat gcgaccgcct gtgacctggg ctgcgttaat aattgcctct
     3241 ttttcaactt ccgcaaatga cagactggtg gaaaggcggg tggcgctgac atcatctgtc
     3301 gcctgctcgg taaacagatg ttcaggtaaa tcactgacgc gaatgcgccc gttatcacta
     3361 ctcagagcaa gattctcgat gacgctgtaa agttcaaaat cgttgccagg ccatgcacag
     3421 gaaaccaggc gagcgagggc atcgtcatca attttcagcc gcgtagagaa gcgtttttca
     3481 agactgtgta atttgttatt caccagcgcc ggaatgctgc cacgccgcat acgcagaggc
     3541 gggatggtaa tttcaaatgc atgcagcgcg taatacagct ggcgactaaa gcgattttgt
     3601 tccaccagca ttgcgaggtc cgcggtcgtt gtggcaatca ctttgacatc aatgggtatt
     3661 aaacgccgcg catccagtcg cgtgataaca ccctgcttga taacctgaag caaagcagac
     3721 tgtaactcca ccgccagata ttcaatcttt tcaagaaaca gcgtgccgcc gtgcgccagt
     3781 tccagccgac tcagacggcc attttcatta tccgtgcgat cgccaccaat aaattcttcc
     3841 gccagcgccg catcaccata taactcacaa ttgacggcaa tataaggccc cgctgcacgc
     3901 tcgctttcat tatgaattgc ctgacttagc agtgccttgc ccacgccctc ctctccacaa
     3961 agcaggacag gaaagctact gcgcgccgcc tggcgaccaa aatgaatcaa gcggcgggtt
     4021 tgcggatcct cttgtggcat atgagcgaaa gtatggctga cttttcctaa ttgactggtc
     4081 attaactgcc gcatctgttc cacaggatgg agcaacaaaa taaagctggt tccctgcgtt
     4141 tctattatcg gtttaagggt tatcaccgca tcaataaact gatgctgact ttcaaaggtt
     4201 gcttctacgt gtttgagcgg atgtgcctgt tttattgctt gttgcaatac ggcgggtaac
     4261 gttaagagtt cagtgatcgc ccttccctga cttgccgtcg cgtcaaggcg caagacccgc
     4321 gccgcctggg cattgataaa ttgcaaatga ccctgctcgt cccagctaat cacaccatca
     4381 tccatacttt ctaacagggc attaagttga tttaaatgac ggttagtttc agcgagcaaa
     4441 ctgtccgtca gcagtaaatt tccgacctcg cgtgcgattg ccaacgtcaa cggcaaatca
     4501 gctgcggtag tttgctcaac cggacacgcc agcgctattg ttcccgtcaa tcggcccttg
     4561 ctgtcaaaca acggcgttgc acaaaaggcc cagttccaga gtgcctgttt gaaatgttga
     4621 tcggccatcg ttttcacggc ctgaccagag atagccgcta acgatagcgc acaagtacca
     4681 ataattccct cggcgcaata cgtgccgtca ttgaatccca gtgtacttag ttgctgcaag
     4741 gtttgcggat cgccattacg gctgagaatg caggcggttt catcgaggat aagcaacgca
     4801 cactctcgcg gtgccatata ttcccaggca tcttccagcg cagcctgccc gagcgtcagc
     4861 atcgctttct tacgccgata aatagaagca aatgtcacgc cctgggcctg gtgtggtacg
     4921 ttccatgtct cccgtttcat cagcttattg catctctccc aggaggttgc aattaaggga
     4981 gatatgcccc gaccatcgtt gttaaaagcg ccactcatat c
//
LOCUS       DACBVI010000237         4980 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.240, whole genome shotgun sequence.
ACCESSION   DACBVI010000237 DACBVI010000000
VERSION     DACBVI010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.240"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            10..945
                     /locus_tag="IU325_21760"
     CDS             10..945
                     /locus_tag="IU325_21760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAP5811616.1"
                     /translation="METCLSRKSSEDKPQIFTTLRNIDLNLLTIFEAVYVHKGIVNAA
                     KVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLESILGALD
                     IEGSYDKQRTITIATTPSVGALVLPVIYRAIKSHYPQLLLRNPPISDAENQLSQFQTD
                     LIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHILLLPEGQNF
                     SGLRQRVQEMFPDRQISFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPF
                     PSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            1154..2119
                     /gene="lipA"
                     /locus_tag="IU325_21765"
     CDS             1154..2119
                     /gene="lipA"
                     /locus_tag="IU325_21765"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HAP5811617.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(2221..2424)
                     /gene="tatE"
                     /locus_tag="IU325_21770"
     CDS             complement(2221..2424)
                     /gene="tatE"
                     /locus_tag="IU325_21770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HAP5811618.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2553..3341)
                     /locus_tag="IU325_21775"
     CDS             complement(2553..3341)
                     /locus_tag="IU325_21775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HAP5811619.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            3437..3820
                     /gene="crcB"
                     /locus_tag="IU325_21780"
     CDS             3437..3820
                     /gene="crcB"
                     /locus_tag="IU325_21780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HAP5811620.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3874..4083)
                     /gene="cspE"
                     /locus_tag="IU325_21785"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3874..4083)
                     /gene="cspE"
                     /locus_tag="IU325_21785"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAP5811621.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(4258..4818)
                     /gene="pagP"
                     /locus_tag="IU325_21790"
     CDS             complement(4258..4818)
                     /gene="pagP"
                     /locus_tag="IU325_21790"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HAP5811622.1"
                     /translation="MNVSQYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGQSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         1332 a         1260 c         1102 g         1286 t
ORIGIN      
        1 aataatcaaa ttgaaacctg tttaagcaga aagtcgtcag aagataaacc acaaatattt
       61 acaaccttac gaaatattga tcttaacctt ctgactattt ttgaagctgt atatgtacat
      121 aaagggatcg ttaatgcagc gaaagtgctt aatctgacac cctcggcaat cagtcagtct
      181 attcagaaac tgcgcgttat attccctgac ccattgttta ttcgcaaagg ccagggtgtc
      241 actcctaccg catttgcgat gcatctacat gagtatatca gtcagggcct tgagtccatt
      301 cttggcgcgc tggatatcga aggaagctat gataagcaac gaacgataac cattgcgacc
      361 accccctcgg tcggagccct ggtccttcct gtcatctatc gggcgattaa atctcactat
      421 ccgcagcttt tactgcgcaa cccccccatc agcgacgcgg aaaaccaact cagtcagttt
      481 caaaccgatc tcatcatcga taacatgttt tgcaccaatc gtacggtgca acatcatgtt
      541 ctgttcaccg acaatatggt gttaatttgc cgcgagggaa atccactact ctctttagaa
      601 gatgacaggg agactatcga caacgctgcg catatactcc tgttaccgga agggcaaaat
      661 ttcagcggtc tgcggcagag agttcaagag atgtttccgg accggcaaat cagtttcacc
      721 agctacaaca ttttgacaat cgctgcactg gttgccaaca gtgacatgtt agcgattatt
      781 cccagccgtt tttataacct gtttagccgt tgctggccgc tggaaaaact gccttttccg
      841 tccttaaatg aggagcaaat agatttctct atccactaca acaaattcag cctgcgtgat
      901 cccatcctgc acggggtaat agatgttatc cgtaatgcat tttgaaaaag tattgttaca
      961 gccagatcac agcgaagaac gaaaaaatgg cacaatccaa caacaatttt acattttcgc
     1021 gaccgctttg gctgctttca ggtccgtttc aatgatatac tgccagtcgt taattcaaaa
     1081 atagttgata attacaacaa tctattgaat tgaaacgctt tccttcgtaa ttcgcaactg
     1141 gaacacgcac gctatgagta aacccattgt gatggaacgc ggtgttaaat accgcgatgc
     1201 cgataagatg gcccttatcc cggttaaaaa cgtggcaaca gagcgcgaag ccctgctgcg
     1261 caagccggaa tggatgaaaa tcaagcttcc agcggactct acacgtatcc agggcatcaa
     1321 agccgcaatg cgcaaaaatg gcctgcattc tgtctgcgag gaagcctcct gccctaacct
     1381 ggcggaatgc ttcaaccacg gcacagcaac gtttatgatc ctcggcgcta tttgtacccg
     1441 ccgttgtccg ttctgtgacg ttgcccacgg tcgcccggta gctccggatg ccaatgaacc
     1501 agtgaaactg gcgcagacca ttgccgatat ggcgctgcgt tatgtggtta tcacctccgt
     1561 tgaccgtgat gacctgcgcg atggcggtgc ccaacacttt gcggattgca ttactgccat
     1621 tcgggaaaaa agcccgcaaa tcaaaattga aactctggtg ccggatttcc gcggtcgtat
     1681 ggatcgtgct ctggatattc tgaccgccac accaccagat gtattcaacc ataacctgga
     1741 aaacgtaccg cgtatttacc gtcaggtacg gcctggtgcg gattacaact ggtcgctgaa
     1801 gctgctggaa cgctttaaag aagcgcatcc ggaaatcccg accaagtctg gtctgatggt
     1861 gggtctgggt gaaaccaatg aagaaattat tgaggtaatg cgcgacctgc gccgtcatgg
     1921 tgtgacgatg ctaacgctgg ggcaatattt gcagccaagc cgccatcacc tgccggttca
     1981 acgttacgtt agcccggatg agttcgacga aatgaaagcc gaagcgctgg cgatgggctt
     2041 tacccatgct gcatgcggtc cgtttgtccg ctcgtcttac cacgccgatt tgcaggcgaa
     2101 agggatggaa gttaagtaat cctgtaataa ttacttacat ctatctgata aaaaacccgc
     2161 tttttgaagc gggttttttt gtatcgaaca agatattgag ggagcgtcct gctcgccacg
     2221 tcactcttta tgagagagct tttcagcctg aagatcaacg tctgcgcctt ttttcgccgc
     2281 agcatcgtca tcattcatcg ccttcttgaa ccctttaatg gccgctccaa ggtctccgcc
     2341 cagcgtacgt aacttcttag tcccaaacag cagaacgacc agcgccgcaa ctaccagcag
     2401 tttggtaata ctaatctcac ccatagatac cttcttgaca tataaccggc aacagaacgc
     2461 gccttaaaaa ccagagaata ttaacggtca ttcgacgcgc gcacacaata gcaatctgta
     2521 acaaggtgaa tcaagcgccg agtaaaaaaa catcataata attgcggcgg cgcaaagcga
     2581 cggttgttta aaacgggaag ttgcgcgcgc acctgacgca cacgttcggg cgtcacttcc
     2641 gccataatga gtgcaggcat ttctgacgct gccgcaatgg tgacgccaaa gggatctata
     2701 atccggcttt gcccgatatt tttgttcccg cactcccccg ccgccaccat ataacaggtg
     2761 gtatccagcg cacgagcggc aagcaacgtt gaccagtgat gctctttgag cggcccacga
     2821 acccaggcgg caggaagtac caggatttca gctccctgta atgcctgtgc cagcgccagc
     2881 tctggaaagc gtaagtcata acaggtcatc agaccgacct tcatcccttc cacctccagt
     2941 aacggagcta tttcattacc agcatcaaca tggcgtgatt cctgaatggc aaatgcatca
     3001 tagagatgca gtttggcata acgggcgacg atgtttcctg cctgtagcgc caccagcata
     3061 ttccatgcgc gccccggcgt tgaaggaaca tgaatcgtca gaattgtcgt catcatgtta
     3121 cgtttacttt ctcgccgtaa acgtccgagg aattcgcctt ccagcagctg tgctgattta
     3181 accgatagat ctgcatcatg atcatcgcgc gccagcaatg cttccggcag gacaaacagc
     3241 gatacgtcgt tttccgccgc ctgcgccatc aacgaggcac aaatctcagc gttcttttcc
     3301 cacacagatg taacagcaaa ctgtcctgcg gcaaccagca ttgccacctc ctcctgtcag
     3361 agtcgtaaaa ttcgccaacg gcgattattc ggcgttacac ttatcactca tacaaatcaa
     3421 atagcaggat tttgcagtgt tacaacttct tttagcagtt tttattggcg gtggtacggg
     3481 aagcgtggcg agatggctgt taagtatgcg atttaacccg ctgcatcaag cgattccgtt
     3541 ggggacgctg gctgcaaacc tgattggggc attcatcata ggaatgggat tcgcctggtt
     3601 tagcaggatg acgaacattg atccagtgtg gaaagtatta atcaccaccg gattttgtgg
     3661 cggtctaaca accttctcaa cattttcggc agaagtggtg tttttgttac aagagggccg
     3721 ctttggctgg gcgttactga acgttttcgt caaccttttg gggtcttttg ccatgaccgc
     3781 actggctttc tggctgtttt ccgcctcaac cgcacactaa agaccaaaaa aaacccgctg
     3841 attaagcggg ttttgaattc ttgctgacgt atcttacaga gcgattacgt ttgcagcaga
     3901 agggcctttg gcaccgttag tgatttcgaa ctctacgcgc tgaccttcag caagagtttt
     3961 aaaaccatta gtctggattg cagagaagtg tacgaacacg tctttgctgc cgtcttccgg
     4021 agtaatgaaa ccgaatcctt tggactcatt aaaccactta acgttacctt taatcttaga
     4081 catcaaaatt acctttacat gaaaaataga cacaaatgct gtgtcggtta ccagtacacc
     4141 aattgtggta cgctttgtcc agtgcaactt tactaaaaag tgataaaagt cgcgttattt
     4201 ttgatgttac ttattttagt tattgatttt aaagaagtta ctaaaacttc atttgtctca
     4261 aaactgaaag cgcatccagg caaagtacac attgccattg ttgtaggtac ccggaatgta
     4321 ggtcatctga aaagtcactg ggccataacc cacggaggcc aatggcagta gaaccgggag
     4381 agggatgtaa ttccagttat cgcgtgccgt tacgccagcg gtgaatccca gacctaaatg
     4441 aaaattttca tccgccagcg gtcgccaggt actttcccat ccgtatccgg caatcggttc
     4501 ccatttgttc cacgagtcct taaatgccat ggcatacagg ccatgccagt ttcctttttc
     4561 atcccaacgc gactgaccaa aaccgccacc ccacggtcgc tcgttatagc gatcggtttt
     4621 ttctttgtcg taagcgaaac gtgcatgcca ggtgatggca ggaatatata aatcataatg
     4681 ttcaggctgt tgccaggttt gtgcaatatt ttctctaaac gttgtcatcc actcatctgc
     4741 gttagcaaaa actttaccaa cgctgattaa ctgaataaaa acaaaggaaa agatagcgac
     4801 atattgactc acgttcattt gtgaccataa aacatttatc aaaaatctac tactagcata
     4861 gcaaagctac aattaacata accttaatag tcccaacatg aagaataatc tgaatagctg
     4921 ctctatttat ttccgcttgt tcaagataaa tatttccgcg catcgattaa agatgattcg
//
LOCUS       DACBVI010000238         4965 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.241, whole genome shotgun sequence.
ACCESSION   DACBVI010000238 DACBVI010000000
VERSION     DACBVI010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.241"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..447)
                     /locus_tag="IU325_21795"
     CDS             complement(<1..447)
                     /locus_tag="IU325_21795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP5811623.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQR"
     gene            567..1376
                     /gene="proC"
                     /locus_tag="IU325_21800"
     CDS             567..1376
                     /gene="proC"
                     /locus_tag="IU325_21800"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP5811624.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGVMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(1393..2508)
                     /gene="adrA"
                     /locus_tag="IU325_21805"
                     /gene_synonym="dgcC"
     CDS             complement(1393..2508)
                     /gene="adrA"
                     /locus_tag="IU325_21805"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HAP5811625.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPSPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVMLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(2610..2930)
                     /gene="psiF"
                     /locus_tag="IU325_21810"
     CDS             complement(2610..2930)
                     /gene="psiF"
                     /locus_tag="IU325_21810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HAP5811626.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(3049..4464)
                     /gene="phoA"
                     /locus_tag="IU325_21815"
     CDS             complement(3049..4464)
                     /gene="phoA"
                     /locus_tag="IU325_21815"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HAP5811627.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPDNALEKGGRGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(4565..4825)
                     /gene="iraP"
                     /locus_tag="IU325_21820"
     CDS             complement(4565..4825)
                     /gene="iraP"
                     /locus_tag="IU325_21820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HAP5811628.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRNYVKKLLKHPRQ"
BASE COUNT         1141 a         1340 c         1235 g         1249 t
ORIGIN      
        1 acgttgcact tccagccacc acttctccag ctccgcggca aaggcctggc ggtcacgttg
       61 tgaaaggcta tctggtccgc cggtctggat cccactggca cgtaaggtat ccataaaatc
      121 gcgcatcgtc aggcgctcac gaatggtcgc tggcgtgtaa cgttcgccgc gcggattaag
      181 cgccgcagcg cctttctcga tggcttcagc agccaaaggt atatctgcgg tgatcaccaa
      241 atcgcccgct tcacactgcc ggacaatttc gttatcggca acgtcgaaac ctgccgcgac
      301 gcgcagcgta cgaataaatc gcgatggcgg cacgcgtaaa ctctgatttg ctaccagtac
      361 cagcggcatc tgcatacgtt ccgccgcgcg atacaaaatc tctttaatta cattgggaca
      421 cgcgtcggca tccacccaaa ttgtcataaa gtcatccttt gttgggtaat cctctattgt
      481 gtcgcgcttt tgccttccgg catagttctg tttatgcttc tgccagcgat tatcaaaaca
      541 atgaatttca cggcaggagt gaggcaatgg aaaagaaaat cggttttatt ggctgcggca
      601 atatgggaaa agccattctc ggcggtctga ttgccagcgg tcaggtgctt ccagggcaaa
      661 tctgggtata taccccctcc ccggataaag tcgccgccct gcatgaccag ttcggcatca
      721 acgccgcaga atcggcgcaa gaagtggcgc aaatcgccga catcattttt gccgccgtta
      781 aaccaggcgt catgattaaa gtgcttagcg aaatcacctc cagcctgaat aaagactctc
      841 tggtcgtttc tattgcagca ggtgtcacgc tcgaccagct tgcccgtgcg ctgggccatg
      901 accggaaaat tatccgcgcc atgccgaaca ctcccgcgct ggttaatgcc gggatgacct
      961 ccgtaacgcc aaacgcgctg gtaaccccag aagataccgc tgatgtgctg aatattttcc
     1021 gctgctttgg cgaagcggaa gtaattgctg agccgatgat ccacccggtg gtcggtgtga
     1081 gcggttcttc gccagcctac gtctttatgt ttatcgaagc gatggccgac gccgccgtgc
     1141 tgggcgggat gcctcgcgcc caggcgtata aatttgccgc tcaggcggta atgggttccg
     1201 caaaaatggt gctggaaacg ggagaacatc cgggggcact gaaagatatg gtctgctcac
     1261 cgggaggcac caccattgag gcggtacgcg tactggaaga gaaaggcttc cgtgccgcag
     1321 tgatcgaagc gatgacgaag tgtatggaaa aatcagaaaa actcagtaaa tcctgatgac
     1381 ttttgccgga cgtcaggccg ccacttcggt gcggttacgt ccggctttct ttgctttgta
     1441 gagcgccaaa tctgccgatt tcaaccactc gcgatagtga ctcatttgtg ggttcagcgg
     1501 cgcaaccccc acactaatcc gtaacatcac ctgcggcgta ttcggcaggc gtaacgtatt
     1561 taatccttca tgcactcgta acatggcggt aatggcgctc tcagctggcg taccggacat
     1621 gattaccgca aactcatcgc cgccaaaccg accaatcaca tcgctaccgc gcagggttat
     1681 ttgtaattgt cgggtaagcg ccacaatcgc ttcatcgccc acatcatggc cccaggtatc
     1741 gttgatgctc ttgaaatggt cgatatcgat aatcagtaat gtcgcatcgc ggttatgccg
     1801 ccgacagtta tcaaattcat tgcgtaacat agtttcccaa tgacgtcggt tatacacgcc
     1861 cgtcataccg tcgcgggtac tcatgacctg caacctgcgt ttatgttccg ccagtttagt
     1921 cgccgtctgg tagctgaccc agccaaacag cagaggataa atgacgataa tgggaaggga
     1981 gagccaccat tccagcggcg cactattgaa cgacacggtg atgcccgtca gttcaagggt
     2041 gacaagacag gaaaccacca tcaataccag acccgcgaca aacagacggg ggccgcctgc
     2101 ccccatcaaa ttcagacaca taatcatcaa catcgcggtg gaaggcagca cgtttacgcc
     2161 cattacgcct acccacattc ccgctaatac tgcatcggtt tttaagttgt aaatttcccg
     2221 gctaagcgga tcgacggccc tgctcgctat ctgccaggct aaatgcggcc agacgaacgc
     2281 ccagccgacc aacaccagcc accaccagcc cggcgacggg tgcgaaacca gcgttgaagc
     2341 aatcggtaag aacatgccag ccaggccaac cgcgcggggt agtctgacgc gacgggcgaa
     2401 gcgcagcccg gatcgctgat gttcgttttg aggagttaaa ggaggttcct ccccatgcgc
     2461 cgccgctttt ttgaaaaagt tttcatcatt cattattttt gggaacattc agaaacaatt
     2521 ttcccaaatt atagagacgg ggctaaaggc agaagtatga aatttcgggt gagccatttt
     2581 cgtagctcac ccgttagcac tatcaggtat caggcggctt tcttgaggca ggcgctcata
     2641 aacttattac gatcatcacc tttcagagat tgttgcgttg cttgattatt acattcgcgc
     2701 atcttttgct gctgtggcgt caaacttttt tcaccaggcg cagacttgct gttcttcagg
     2761 caatcactca tgtaggtctt acgagcatcc cctttcaacg cctgcgccgt cgcctgctga
     2821 ttacaggagg tcatacgctg ttgttgcggg gttaaggttc tctcggcagc gccgacggtg
     2881 gttaaaaaaa ccagaccgaa aagcaaggta accagtaatg ttattttcat agcaccatcc
     2941 ctcttcatgt tttaaccatg agcgtatgcg cccgtgatct gccattaagt ctggttgcta
     3001 acagcaaaaa aaccacccgg cagcgaaaat tcactgccgg gcgcggtttt atttcagccc
     3061 cagggcggct ttcatggtgt agaagagatc ggtctggtcg gttagtccaa caacattggc
     3121 ggcatgcggg ccatacgccg caatacgcaa ctgactgccg gtatgttctt gtgaatcctc
     3181 ttcggagttc ccgtaactca tcaccatcac tgcgccatct ttggtattta gtgcctgggt
     3241 gaggcccggc gctttggtgt ccggcgcgac aatctggctg gcgtgggcgt gatcagcggt
     3301 gactatgacc agcgtgttgc catccttttt agcgaattcc agcgcacgtt gtacggcttc
     3361 atcgagatcg accgtctcgc caatttgccc acaaggattc gcagcgtgat cctgtttatc
     3421 gatggatgca ccttcaactt gcaggaaaaa gcctttctca tttttactca acaattcaat
     3481 ggctttgtcg gtcatctgcg ccagggtcgg tacgctgtca ttacgttgcg gattaggcgt
     3541 acaggtaact gcgggcttgt cgatattgcc gtggtacgtt gctttcggtc ctagccagcg
     3601 cactggcata ttgccgtcag caaacaggcc aagcaggggt ttttgctgat tcgcttccgt
     3661 caccgcattt agcgatgcag catcactcac cagctgataa ccacgcgcct gtgcctgttc
     3721 acgcagcgtt tttccctgcc attcgccagc ggttgccgtt tcagcaaagg tttttgcgcc
     3781 gccgccaagc gtaacatcgg cacgggcgtt aagcagctgt tcggtaatcg atcctctccc
     3841 gcctttttcc agagcgttat ccggacattt ttcactggtc gcgctcggac cgtagcattt
     3901 gcgcgaggtc acatgcgcca ccagcgcggc gggcgtggca tcctgcaact ctgcggtaga
     3961 aacgttaccg gtcgccagac ctgcggcttt tgccatttcc agaatcgttg ggtgatcttt
     4021 ttcgtgaata tcgacgccca gcgcaccgtt ataggttttg acaccagttg accaggcggt
     4081 tgctgatgca gccgagtcgg taacgtagtc cggtttgccg gtttttttat tcagcgcata
     4141 gtgtgtgtat tgtccggtaa gcggtaaggc atcgatacct ttaaaaaagc cgcccgcacc
     4201 ttcggcataa ttgcgtgcag cagtaatttc cgagtccccc atcccatcgc caatcagcaa
     4261 aataatattt ttggcaggtt tatcgctaag agaatcacgc agagcggcgg tctgatcacc
     4321 cgttaagcgg cgagcaccgc cgggtgcagt aatatctccc tgagcagccc ggttttccag
     4381 aacaggcatt tctggtgtcc gggcttttgt cacaggggta aacagtaacg gtaagagtgc
     4441 cagtgcaata gtgctttgtt tcactttatt ttctccatgt acaaatacat taaaaaataa
     4501 aaacaaagcg actataagtc tcggccgtga cagctttatg acagctgttg aaaagattaa
     4561 ctttttactg acgaggatgc ttcaataact tctttacata attgcgcagc agctccgtat
     4621 cgtcgtcagg aatgctggca tcgggcttta cctcgtacag cgccccctct acctgatcaa
     4681 tcaacctttg ttggtcattt tgcgccatat tgcgaagcat tgcagtgacg ataatctcca
     4741 aagcttctac ctgcgcgcac agttctttcg actcttcttc tttttgggca agcttaaata
     4801 acaactcagc aatgagattt ttcatgtctg tatttcctta tccaaagtat ggagaagtta
     4861 acattatgtt tcacaacttc atagtgtctg gaagtattat ttttatgaca aaggaaatac
     4921 tttgcgcaaa tattttttgt ttgattacca gcgaaacgtt tctct
//
LOCUS       DACBVI010000239         4845 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.242, whole genome shotgun sequence.
ACCESSION   DACBVI010000239 DACBVI010000000
VERSION     DACBVI010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.242"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(49..810)
                     /gene="kduD"
                     /locus_tag="IU325_21825"
     CDS             complement(49..810)
                     /gene="kduD"
                     /locus_tag="IU325_21825"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HAP5811629.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(840..1676)
                     /gene="kduI"
                     /locus_tag="IU325_21830"
     CDS             complement(840..1676)
                     /gene="kduI"
                     /locus_tag="IU325_21830"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP5811630.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1963..3144)
                     /locus_tag="IU325_21835"
     CDS             complement(1963..3144)
                     /locus_tag="IU325_21835"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HAP5811631.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVS
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3399..4628
                     /locus_tag="IU325_21840"
     CDS             3399..4628
                     /locus_tag="IU325_21840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAP5811632.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1273 a         1235 c         1052 g         1285 t
ORIGIN      
        1 ataaaaaacc ctgccatgcg gcagggtcat aaaagtaaga gaaatgaatt aacgagccag
       61 ccaaccgcca tccacggcaa tggtataacc attcacataa tctgaagcgc tggaggcaag
      121 gaacactacc ggccccatca ggtcactcgg cagtccccaa cggccagctg gaatgcggtc
      181 gagaatttcc gcgctacgtt gttcatctgc ccgtagttgt tgagtattgt tggttgccat
      241 gtaacccgga gctatcgcat taacattaat gttgtgttta gcccattcat tcgccatcaa
      301 tcgcgtcacg cccatcacgc cgctttttga tgcggtataa gaaggcacac ggatcccgcc
      361 ctggaaggag agcattgacg cgatattgat aatcttgccg ccattgcctt gcgcgataaa
      421 gtgtttcgcc gctgcctgag acatgaagaa tacgctcttg atattcaggt tcatgacatc
      481 gtcccagtct ttttcgctga actcgagagc atcttcgcgc cgaatcaatc cggcgttatt
      541 caccaggata tcaatatgac caaactcagc taccgcgcga tccagcagtg ctggaatgcc
      601 atcaatcttt cgcagatcgg cggtcaggct taaaaaacga cgccccagcg ccgtgacctg
      661 cttgatggtt tcagtcggtt caacgatgtt aatgccaaca atgtcacagc ccgcttgcgc
      721 cagccccaac gccatcccct gacccagtcc agtatcacaa ccagtgacga ccgcaacttt
      781 accttcgaga gaaaatgcac ttaaaatcat aacaatacct tattcgttta tgcccacaac
      841 tagcgcaaat ctttaacggc cacatggtcc atatcatcaa agacctggtt ttcaccgacc
      901 atgccccaga taaaggtata agctttggtt ccgacaccgg aatggatcga ccagctcggg
      961 gagatcaccg cctgctcgtt atgcatcaca atatgacgcg tttcttgcgg ctgccccatc
     1021 atgtggaaaa cgcaggcgtc atcatccata ttgaaataga aataaacttc catccggcgc
     1081 tcgtgggtgt gacacggcat ggtgttccac aagttacccg gagccagctc cgtcagcccc
     1141 atactcaatt ggcaggtttc cagtacatcc gggacaaaat atttgttaat cgtgcgacgg
     1201 ttactggtga ggttatcgcc taacgtgact ggagatactt cgtccggtgt gacttttttg
     1261 gtgggatacg tcgtatgcgc gggtgcgcaa ttgtaataaa acttcgccgg agtggcggta
     1321 tcaatactgg caaagacaac ttcttttgca cctttaccaa catacagggc gtcgcggtga
     1381 ccgatttcat agcattgacc atcgacagta atcgtaccgg caccgccaat attgataaca
     1441 cctaactcgc gacgttccag gaaatagctt acgccgagtt gtttaccaac ttccccgcca
     1501 acggaaaccg ttttagttac cggcataatg ccgccaacaa ttatacggtc aatgtggctg
     1561 taaaccatgg tgtactcatc ggcgacaaat accttttcaa ccaaaaattc attgcgcagc
     1621 ccctgggtat ccagcgtttt tgcatgcgca ctgtggatgc tctgtcttac gtccacatca
     1681 acctccgata atcagtgaag cgtgaaaata gaaaacgata gtgaaacgct gtttcgtttt
     1741 ctctataagg ggatgatatg cccacgggat agggttttca attaaaaata aaacaatgtt
     1801 tcatttttaa gttaaggact gaaaaaagtg cagtgtcgat cacgaatatg tcatgagaat
     1861 ggaaaaatca gggagccctg aaaatgtatg tcgactcgcc agccaccatt attcaacaga
     1921 ttaatgacgg ctgatattta tcggtaagga ggaaatgcag agctattcgt cacgttcaat
     1981 ggtcaatgcc acgccctggc ccccgccgat acaaagcgta gccagtcctt tgcgggcatt
     2041 acgtttcacc atttcatgaa ccagagaaac gaggattcgg caaccggaag cgcctattgg
     2101 gtgaccgagt gcgatcgcgc caccattgac attgacccga cgctcatccc attcaagcat
     2161 cttgccaacc gaaagcgcct gtgcagcaaa cgcttcatta gcctcgataa gatcgacatc
     2221 agccaactgc cagccaacac gctccaggca acggcgggtc gcatacaccg gcgcaattcc
     2281 catcaatgcc ggatctacac caacactggc aaatgcgcgg atgcgggcca gcacgggtaa
     2341 attcaacgct cgtgctttgg cttcgctcat catcattact gcagctgcgc catcgtttat
     2401 ggatgatgca ttacccgctg tcacagagcc gagactatca aatgaaggat ttaaacgggc
     2461 taagccttct gcactggcgt cagtgcgtgg ctgttcatcg gtatcaacaa ccaacgtctg
     2521 accgttactt tgggtcatta ccgggacgat ctcatcttta aatcgtccgg cgtcaatcgc
     2581 cgctcgcgct ttttgttgcg agctaagtgc gtaagcatcc tgcaactgac ggctgatgcc
     2641 atattcgcga gccagatttt cggcggtgac accaatatga taatcattga aggcatccca
     2701 caacccatcg tgcacaagac tgtcaaccaa ctggctattg ccaagctgtg caccggtgcg
     2761 gctatcagtc agaacatgtg gtgcgcgact catgttttcc tgaccaccgg cgatgacaat
     2821 atcagcctcg ccacactgta tcgcctgagt agccagatgc agtgctttaa gcccggaacc
     2881 gcaaacgtca ttaatcgtga ttgcagaaac gctattagga agaccacctt taatagccga
     2941 ttgccttgcc ggattctgcc ctgcccctgc agtcaacacc tgaccaagaa tcacttcatc
     3001 caccgcatat gcagaaacac cggtacgttc tattaatgct ttcacgacca ggctgccaag
     3061 ttccacggcg gaatgacccg ctaacgcacc acgaaagcag ccgataggtg tccgtaacgc
     3121 cccgacaatc acaacgtctt tcatcacaac ctcgcaccaa ttaacagtgc aatagtaaat
     3181 gattgttaat tacagttatc ttaattgttt aaaaaaagtg atttttatca caaaggaaat
     3241 acgcctgacc agcagtcaga gacataactg gcacgtaagg tttgcaacca ctaacccacc
     3301 aatagagagg tagaaagggc gttaatctcc catacttaac ctggtttatg gtaaattgcc
     3361 ctccattttg tttaatttgt agatgatacg ttcagataat gtctaatatt tggtcaaaag
     3421 aagaaactct gtggagtttc gcgctctacg gcacagccgt tggtgcaggc acgctcttcc
     3481 ttcctattca gttaggttcg gcaggggctg tggtcctgtt tattactgct ctggtcgcct
     3541 ggcctttaac atattggcca cataaagcct tatgccagtt catcctctca tcaaaaacat
     3601 cagcaggtga agggataacg ggcgcggtaa cacactacta tggcaagaag attggtaatc
     3661 tgattaccac gctgtacttc atcgcctttt ttgtcgtcgt gttgatatat gccgtggcaa
     3721 ttaccaactc acttacggaa cagctggcaa agcatatggt tattgatctt cgcatccgta
     3781 tgttggtgag tctgggtgtt gtattaatcc tgaatctcat ttttctgatg ggacgccatg
     3841 ccactattcg ggtaatggga tttttggtat tcccattgat tgcctatttc ttatttcttt
     3901 ctatttacct ggtcggtagt tggcaacctg atctattaac tacccaggta gagttcaatc
     3961 agaataccct tcaccagata tggatatcga ttcccgtgat ggttttcgcc tttagccata
     4021 cgcccattat ttctacgttt gccatagaca gacgtgaaaa atatggcgaa catgctatgg
     4081 ataaatgcaa aaaaattatg aaagtcgctt atctcatcat ctgcataagt gtactgttct
     4141 ttgtctttag ctgcctgctt tctattccac cttcgtatat tgaagcggct aaagaagaag
     4201 gggttaccat tttatcggcg ctttctatgc tgccgaacgc cccagcatgg ttgtcaattt
     4261 ccgggattat tgtcgcagta gttgcgatgt cgaaatcatt cctgggtacg tactttggcg
     4321 ttattgaagg tgccacagag gtcgtcaaaa caacactaca gcaggttggt gtaaagaaaa
     4381 gtcgtgcatt taaccgcgca ctatcaatta tgttggtatc gctgattacc ttcattgttt
     4441 gttgcattaa cccgaacgcg atttcgatga tttacgcgat cagcggcccg ctcattgcca
     4501 tgatactttt catcatgcct acgctgtcaa cgtatctcat cccggcgctt aaaccctggc
     4561 gttccatcgg aaatctgatt acgctgatcg tgggtatcct gtgcgtatcg gtaatgttct
     4621 ttagctaata tccattgtga atggttcaaa attgctacaa ttttgaacca ttccagtttt
     4681 cataatgttc atttgttata actccgctct atcacttctc tcgtcatatc ctagtattat
     4741 cagttagtta aacacacaaa gagatctgaa catcttaaat aagactattt aagatgcata
     4801 acttagattc gcaagatatc ttctggtcat tcaaaaacaa tttgc
//
LOCUS       DACBVI010000240         4830 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.243, whole genome shotgun sequence.
ACCESSION   DACBVI010000240 DACBVI010000000
VERSION     DACBVI010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.243"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(340..579)
                     /locus_tag="IU325_21845"
     CDS             complement(340..579)
                     /locus_tag="IU325_21845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V"
                     /protein_id="HAP5811633.1"
                     /translation="MPRHYEIDSAWRASIKREPNGRQTVTTEAFVSQLALINFHWSCR
                     QANQWIETYVTVFKDISTQEGENRTFMLFNPNGGR"
     gene            681..1901
                     /locus_tag="IU325_21850"
     CDS             681..1901
                     /locus_tag="IU325_21850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISKox3 family transposase"
                     /protein_id="HAP5811634.1"
                     /translation="MDEKSLYAHILNLSDPWQVKSLSLDENAGSVTVTIEIAENTRLA
                     CPTCGKSCSVHDHRHRKWRHLDTCQFTTIVEADVPRIMCPEHGCLTLPVPWAGPGSRY
                     TLLFESFVLSWLKISTVDAVRKQLKLSWNAVDGIMTRAVKRGLARIKKPLSARHMNVD
                     EVAFKKGHRYITVISDRDGRALALTDDRGTESLAGYLRTLTDGQLLAIKTLSMDMNAG
                     YIRAARIHLPSAVEKIAFDRFHVAKQLGEVVDKTRQNEHPHLPVESRHQAKGTRFLWQ
                     YSDKWMTESRQEKLMWLRAQMKLTSQCWALKELAKDIWNRPWSEERRSDWQRWLALAA
                     NSDVPMMKNAAKTIGKRLYGILNAMRHSVSNGNAEALNSKIRLLRIKARGYRNRERFK
                     LGVMFHYGKLNMAF"
     gene            complement(1990..2652)
                     /locus_tag="IU325_21855"
     CDS             complement(1990..2652)
                     /locus_tag="IU325_21855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP5811635.1"
                     /translation="MTQTVPKGFVRAYLRASTTEQDATRALDTINTFAIERGLSICNY
                     YIENESGSKLERPELFRLLKDCQQNDILLVEDVDRLSRLVGEDWNTLKKMIRQKDIRV
                     VAVNVPTTWLASGHNDFDSRMFSAINDMLLDMLAAVARRDYEQRRERQQQGIARAKRE
                     GKYKGRQVNQSRYDAINRLIASGSSWSQIQKVLGCSRATISKAVKQQTVNKMNHEPEG
                     RE"
     gene            3033..3695
                     /locus_tag="IU325_21860"
     CDS             3033..3695
                     /locus_tag="IU325_21860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-arginine deiminase"
                     /protein_id="HAP5811636.1"
                     /translation="MGKILLVVSDKGGVGKSTYVANTGSMLVNKGKSVIILKTDKNHD
                     LLSWNEKRTDNGLLTIPVHAAYGNVSNEIKRLSKLCEVLIVDCPGHDSQEFRSALTVS
                     DIVVTLVKPSSDFESETLTSVTEKIRTAQKANAALEPWVLFTRINSAKPRHRKAAIDL
                     DKLLRSDNIWIQPLKTRISELDVYESACNEGAGVHDVSRASSLSTAKAQIELVAQEIG
                     IL"
     gene            3795..4079
                     /locus_tag="IU325_21865"
     CDS             3795..4079
                     /locus_tag="IU325_21865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HAP5811637.1"
                     /translation="MALKLNRPNIDESQQPATNAETARFISGATRAPVAGKPKLMNFR
                     LPAYFEEILEAEAQRTGQSKTTVLKAALAAFDNLDENLKNHWLLESAKLG"
     gene            4285..4596
                     /locus_tag="IU325_21870"
     CDS             4285..4596
                     /locus_tag="IU325_21870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811638.1"
                     /translation="MFIFRYIKTLINIYLLYIQPILGMFLIGIGLWGFYAPDEGTCTN
                     LNIELCAVSAIDVLKPAIFVFLGIITVAGWYFTELQPKLRGEGKYMKVKTGVKPEDFV
                     K"
     gene            4632..>4830
                     /locus_tag="IU325_21875"
     CDS             4632..>4830
                     /locus_tag="IU325_21875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811639.1"
                     /translation="MNKSWLEQQVQNEKLKKVQEEKKDGTIQKGLYRVRIKDKYRWDL
                     WENDFRVVIAYSKDDAELKFTE"
BASE COUNT         1383 a         1113 c         1158 g         1176 t
ORIGIN      
        1 caatgtgcgt aatgaccccc ctgacctcca acccgtcctc atcgtcatca ggtatagccc
       61 tgaagttatc cagcttatcg agttcctgga gacaccgctt ggggtgtagt cgcaatcgca
      121 tgatcctgaa ttcttgttcc aggacacaga gcaccaaaga cccgtcaacg ggcgtgcccc
      181 caaaatctac tatgagcagc gcaccaggct ttatcccttc ccggaaagag taggtatcac
      241 ttcgaaaaaa gaatactccc ggcgctcctg tattacagtg ctgatcgagt gacagcctgt
      301 tttcctggta gtcggttgct ggcgatggaa aacccatttt taacggcctc cgttggggtt
      361 aaaaagcatg aatgtccggt tctctccttc ctgagtggaa atgtctttaa aaacggtcac
      421 gtaggtttca atccactgat ttgcttggcg acaactccag tgaaaattaa tcagcgcaag
      481 ctggctgaca aaggcttctg tcgtaactgt ctgacgaccg ttcggctcac gcttaatgct
      541 cgcacgccag gctgagtcaa tttcatagtg tctaggcatg aaaaacctcc gatcaatata
      601 gctgtatgca tatgggtctt cccttgttgt ggtggctgaa ggcatgataa tggtgtattt
      661 aatcgccaga ggtcaccgcc atggacgaaa agtccctcta cgctcatatt ctcaacctgt
      721 ccgatccgtg gcaggtaaag tccctttctc tcgatgaaaa tgccggttct gttactgtca
      781 ctattgagat cgctgaaaac acccggctag cctgtccgac ctgcggtaaa tcctgttctg
      841 ttcacgatca ccgtcatcgt aaatggcgcc atcttgatac ctgccagttc accactattg
      901 ttgaagccga tgttccacga attatgtgtc cggagcatgg ctgcctgacg ttgcctgttc
      961 cgtgggctgg ccccggaagc cggtatacgt tgctattcga atcgttcgtt ctctcatggc
     1021 tgaaaatcag caccgttgat gctgtcagga agcaacttaa gctcagttgg aatgcggttg
     1081 acggcattat gacccgggca gttaagcgag gtcttgcccg gataaaaaag ccattatccg
     1141 cccgtcatat gaatgtggat gaggtcgcct ttaaaaaagg acatcgttac ataacggtga
     1201 tctccgatcg cgatggtcgg gcgctggcct taacggatga tcgcggcaca gagagtcttg
     1261 ccggctatct tcgcacgctc actgatgggc agttgctggc tatcaaaacg ctctcaatgg
     1321 acatgaacgc gggctatata agagcagcgc gtatccactt acccagtgcg gttgagaaaa
     1381 tcgcctttga ccgcttccat gtggcgaagc aactgggcga ggtagttgat aaaacccgtc
     1441 agaatgaaca tccgcacctc cctgttgaaa gccgacacca ggcaaaagga acccgcttcc
     1501 tgtggcagta cagcgataag tggatgaccg aatcccggca ggaaaagctg atgtggctgc
     1561 gtgcacagat gaagctgacg agccagtgct gggcgctgaa agagctggca aaggatatct
     1621 ggaacaggcc atggagcgag gaaagacgga gtgactggca gagatggttg gcgctggcgg
     1681 ctaacagtga cgttcccatg atgaaaaatg ccgcgaaaac gataggaaaa aggctgtacg
     1741 ggatcctgaa tgcgatgcga cacagtgtct caaacggaaa tgcggaggca cttaacagca
     1801 agatcaggct gctgaggata aaagccaggg gataccgaaa ccgggagcgc tttaaactgg
     1861 gggtgatgtt ccactacgga aagctgaata tggcgttctg agccttccca ccatgatcgg
     1921 ggaagaccca tgcatataca gtataaagaa tcagttttat tcttcaacac actgtttatt
     1981 atgttgtaac tactccctac cttcaggttc atggttcatt ttgttaacgg tctgttgctt
     2041 cacagctttg ctgatagtcg cccggctaca acccagcact ttttgaatct ggctccagga
     2101 gctgccgctc gcaatcagcc tgtttatggc gtcatagcga gactgattaa cctggcggcc
     2161 tttatacttc ccttcccttt tcgccctggc gatcccctgc tgctgccgtt cccggcgctg
     2221 ttcatagtct cgcctggcga cggcggccag catatccagc aacatatcgt ttatggccga
     2281 aaacattcgg ctgtcaaaat cattgtgacc cgatgcgagc caggttgtcg gaacgttgac
     2341 ggccacaacc cggatatctt tctgccggat cattttcttc agtgtgttcc agtcctcccc
     2401 taccaggcgt gaaagcctgt ccacatcctc taccagcaag atatcgtttt gctgacaatc
     2461 tttcaggagc cggaacaact cagggcgttc aagcttagaa cctgattcat tttcaatgta
     2521 gtagttacag atgctcaggc cgcgctcgat ggcgaaggta tttatcgtgt ccagggcgcg
     2581 tgttgcatcc tgttcagtcg tggatgcgcg taagtaagct ctgacaaagc ctttcggtac
     2641 tgtttgagtc attataggga accagttcat tttgagttaa ccagacacac ccatacagta
     2701 cccatttcag ttggttcagg gaaggcacac tcaaaaagaa ccagcaaaca gaccccagcc
     2761 ctatagtgac aacgtcacac taaaaattta caacataaag atgatgtaaa gacacaccaa
     2821 gatttacacc ataaagcgca cataaagaca cagcaacatt taccatgtaa agacaccata
     2881 aagataaata tatctttatt tgtgctttac atggtaaagc cattgcatta taaagacact
     2941 gtaaagacaa aagtatcttt acgaaaaaca caccatgtaa agacattcat atctttacat
     3001 gagctttaca acataaagag agggattctg aaatgggtaa aatcttgctt gtcgtatcag
     3061 ataaaggtgg ggtaggcaaa agcacctacg tggctaacac aggctcaatg ctggtcaaca
     3121 aaggtaagtc ggtaattatc ctgaagacag ataaaaacca cgatctgttg agctggaacg
     3181 aaaagcggac agataacggc ttactcacta tcccggttca cgcagcttac ggaaacgtca
     3241 gcaacgaaat taagcgcctt agtaagctgt gtgaagttct cattgtcgat tgccccggcc
     3301 acgacagcca ggagttccgt agcgccctga cagtatcaga tatcgtggtg acactggtta
     3361 agccgtcctc tgatttcgaa agcgaaacac tgaccagtgt tacagagaaa atccgcaccg
     3421 ctcagaaagc taacgcagct ctggaaccgt gggtgctgtt caccagaatc aactcagcga
     3481 aaccccgcca cagaaaagcg gccattgatc tggataaatt actgcgttcg gacaacatct
     3541 ggattcaacc tctgaaaacc cggatatccg agctggatgt gtatgaaagc gcctgtaatg
     3601 aaggggcagg ggtacatgac gttagccggg catcgagcct ttcaacagcg aaagcacaga
     3661 ttgaacttgt ggcacaggaa attggcattt tatagaaata tcaacaatat tcatcgaaat
     3721 ctttacattg taaatacacc ataaagatac tatgatattt acaattaatg cataccgtac
     3781 aaggggaaat tttcatggct ttaaaactaa accgcccgaa catcgatgaa tctcagcaac
     3841 cagcaacaaa cgcagaaacc gcgcgtttta tctccggcgc taccagagcg cctgtagcgg
     3901 gtaaaccaaa gttgatgaac ttcagactgc cagcgtactt tgaggagatc ctggaagcgg
     3961 aggcgcagag aacaggccag agcaagacaa ccgttctaaa ggcagcgctg gccgcattcg
     4021 ataacctgga cgagaacctg aaaaaccact ggctgcttga atctgcaaaa ttagggtagc
     4081 gttatggcta agaaaaagag atatgttgtg atggttagag ataaaccaaa tacagcggca
     4141 atcagcgatt attagcctgg ttagtatggt tcgctaacag gcataataag gccgttgcct
     4201 atgactgcgg agaatggatt gtcgaaccat cacattggtt aagaattgga aatccaaaat
     4261 agaaagaaac ctaaggaaag aaaaatgttc atattcagat atattaaaac tcttattaat
     4321 atctacttgt tatacatcca accgatattg gggatgttcc ttataggtat aggcttatgg
     4381 gggttctatg cacctgatga gggaacttgt actaatctca atattgagtt atgcgctgta
     4441 tcagctattg atgttctgaa gcctgctatt tttgtatttt taggcattat taccgttgcc
     4501 ggatggtact ttaccgaact tcagccgaag ctaagggggg agggcaagta tatgaaggta
     4561 aaaacaggtg taaaaccgga agatttcgtt aagtaaaaac cgagcctaaa gacgttaacc
     4621 gtgggggatt tatgaataaa tcatggcttg aacaacaggt gcaaaatgaa aaattaaaaa
     4681 aagtacagga agagaaaaaa gatggcacta tccaaaaagg attgtacagg gttcgtataa
     4741 aagacaaata caggtgggat ttgtgggaaa atgatttcag agttgttatt gcttattcga
     4801 aagatgatgc agagctaaaa tttacagaaa
//
LOCUS       DACBVI010000241         4601 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.244, whole genome shotgun sequence.
ACCESSION   DACBVI010000241 DACBVI010000000
VERSION     DACBVI010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.244"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..593)
                     /locus_tag="IU325_21880"
     CDS             complement(<1..593)
                     /locus_tag="IU325_21880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP5811640.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(708..783)
                     /locus_tag="IU325_21885"
     tRNA            complement(708..783)
                     /locus_tag="IU325_21885"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(748..750),aa:Thr,seq:ggt)
     gene            complement(790..864)
                     /locus_tag="IU325_21890"
     tRNA            complement(790..864)
                     /locus_tag="IU325_21890"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(829..831),aa:Gly,seq:tcc)
     gene            complement(981..1065)
                     /locus_tag="IU325_21895"
     tRNA            complement(981..1065)
                     /locus_tag="IU325_21895"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1029..1031),aa:Tyr,seq:gta)
     gene            complement(1074..1149)
                     /locus_tag="IU325_21900"
     tRNA            complement(1074..1149)
                     /locus_tag="IU325_21900"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1114..1116),aa:Thr,seq:tgt)
     gene            1511..2461
                     /gene="coaA"
                     /locus_tag="IU325_21905"
                     /gene_synonym="panK"
     CDS             1511..2461
                     /gene="coaA"
                     /locus_tag="IU325_21905"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP5811641.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAINTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2493..3455)
                     /gene="birA"
                     /locus_tag="IU325_21910"
     CDS             complement(2493..3455)
                     /gene="birA"
                     /locus_tag="IU325_21910"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HAP5811642.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILSQLDDGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAE"
     gene            complement(3452..4480)
                     /gene="murB"
                     /locus_tag="IU325_21915"
     CDS             complement(3452..4480)
                     /gene="murB"
                     /locus_tag="IU325_21915"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP5811643.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLKDYRGTVIINRIKGIEIHDELDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1163 a         1161 c         1058 g         1219 t
ORIGIN      
        1 gaatccagga agccagccag ttccaggatt ttcgcttccc actctgcgtc gccttccagc
       61 gctttcagag cagaaccacg aacgatcgga gtgtcgtcgc ccgggaagtc gtactgagac
      121 agaagttcac gaacttccat ttcaaccagt tccagcagct cttcgtcatc aaccatgtcg
      181 catttgttca ggaacacgat gatgtacgga acgcctacct gacgacccag caggatgtgc
      241 tcacgggtct gcggcatcgg gccgtcagtc gcagcaacta ccaggatcgc gccgtccatc
      301 tgagcagcac cggtgatcat gtttttaaca tagtcggcgt gccccgggca gtctacgtgt
      361 gcgtagtgac gggtcggggt gtcgtattca acgtgagaag tgttgatggt gataccacga
      421 gctttttctt ccggcgcgtt atcgatctgg tcgaatgcgc gagcagcacc gccgtaggtt
      481 ttagccagta cggtagtgat tgcagcggtc agcgttgttt taccatggtc aacgtggccg
      541 atagtaccga cgttaacgtg cggttttgta cgttcaaact tttctttaga catcgattgt
      601 ccctctaaga cacggataaa tcggtgatat caccacatca accaggcaac atgcccgact
      661 tgttgaatgc aataaacaga aggaaaaaac agggaggaga aaagaagtgg tgctgatagg
      721 cagattcgaa ctgccgacct cacccttacc aagggtgcgc tctaccaact gagctatatc
      781 agcacatctt ggagcgggca gcgggaatcg aacccgcatc atcagcttgg aaggctgagg
      841 taatagccat tatacgatgc ccgcatcctg gaactcggct acctgatttt cattctgcac
      901 tgaatatcga gagaagctct ctttattcga gccggtaagc gaacttatcg tctcgggcta
      961 cgccatcgcg tggccgaaat tggtggtggg ggaaggattc gaaccttcga agtctgtgac
     1021 ggcagattta cagtctgctc cctttggccg ctcgggaacc ccaccggact tgatggtgcc
     1081 gactaccgga atcgaactgg tgacctactg attacaagtc agttgctcta cctactgagc
     1141 taagtcggca tcaagtagcg cgcattctat ggagacatgc gagttcatgc aactaaaaaa
     1201 ttgcataatt tgttttattg gtcacatttt atgcgacacg atgaagaaac agccgaatgg
     1261 agggcagatt agcacacttt ttcaacatca ttgtgctcaa caatgcgctc ctgctaaacc
     1321 ataattcttt ttatcagatg gaatatctgt cacagtgctt ttcaacgata gcttcctggg
     1381 agagattttt tcttattatt cctccccatc tggtgttacc ctcctgccca ttaacccatt
     1441 caacagaact gtgacgcgcc atggcaaata tcgctttgcc gatagagcta tgaccgccag
     1501 aaacatgctt atgagtataa aagagcaaac gttaatgacg ccttacctac agtttgaccg
     1561 caaccagtgg gcagctctgc gtgattccgt acctatgacg ttatcggaag atgagatcgc
     1621 ccgtctcaaa ggtattaatg aagatctctc gttagaagaa gttgccgaga tctatttacc
     1681 tctgtcacgt ttgctgaact tctatataag ctcgaatctg cgccgtcagg cagttctgga
     1741 acagtttctt ggtaccaacg ggcaacgcat tccttacatt atcagtattg ctggtagtgt
     1801 cgctgtgggg aaaagtacaa ccgcccgtgt attgcaggcg ctattaagcc gttggccgga
     1861 acatcgtcgt gttgaactga tcacgactga tggcttcctt caccctaatc aggtactgaa
     1921 agaacgtggt ttgatgaaga agaaaggctt cccggaatcg tatgatatgc atcgcctggt
     1981 gaagtttgtt tccgatctca aatccggcgt gccaaacgtt acagcacctg tttactcgca
     2041 tcttatttat gatgtgatcc cggatggaga taaaacggtt gttcagcctg atattttaat
     2101 tcttgaaggg ttaaatgtct tacagagcgg gatggattat ccacacgatc cacatcatgt
     2161 atttgtttct gattttgtcg atttttcgat atatgttgat gcaccggaag acttacttca
     2221 gacgtggtat atcaaccgtt tcctgaaatt ccgcgaaggg gcttttacag acccggattc
     2281 ctattttcat aactacgcga aattaactaa agaagaagcg attaatactg ccatgacgtt
     2341 gtggaaagag atcaactggc tgaacttaaa gcaaaatatt ctacctactc gcgagcgcgc
     2401 cagtttaatc ctgacgaaaa gtgctaatca tgcggtagaa gaggtcaggc tacgcaaata
     2461 attttcaggg gagctaatgc tcccctatct tattattccg cactacgcag ggatatttca
     2521 ccgcccatcc agggttttat tattccatcc tgctcaagca ataaagcgcc ctgtttgtct
     2581 attccgcgtg aaatgccaaa tatttcttta tcaccaatga taagtttcac tgggcgatta
     2641 ataaaattat ccagcttttc ccagcgcgaa agataaggtg ccaatccttc ttgctcgaag
     2701 agttccagcg ccgcgcgtaa ttcacgtatg agcatggccg ccaacgtatt acgatcgaga
     2761 ttgatcccgg cttcctgcag cgtgatccac ccttgattaa cgacactctc ttcaacacga
     2821 cgcattgcca tgttgatccc tgcaccaatg actatttgcg ccgcatcgcc ggttttgcca
     2881 gtcagctcca caagaatgcc tgccagcttg cgatcctgca gatagaggtc attaggccat
     2941 ttaacacgaa ctttatctgc acccagcttg cgtaatactt ccgccatcac gataccgata
     3001 accagactta aaccaatcgc cgccgccggg ccttgctcca gacgccagaa catcgacaaa
     3061 tataagtttg cgccaaaagg cgaaaaccat ttccgccccc ggcgaccacg gccagcctgc
     3121 tggtattctg caacacaggc atcgccagat ttaagctctc cgatgcggtc caggagatac
     3181 tgattcgtgg agtcaataac tggcagcacg gctacactgc cgtcatccag ctgactcaat
     3241 atctgttcag cattaagtaa ctggattggc tcgggcaagc tgtatccttt acccggaaca
     3301 gtaaatacat caacgcccca gtcacgcagt gtctgaatgt gtttattaat agccgcccgg
     3361 ctcattccca gcgtttcacc caactgctcg ccagagtgaa attcaccgtt cgctaatagg
     3421 gcgatcaatt tcagtggcac ggtgttatcc ttcatgaaat tgtctccact gcgctcactt
     3481 cacctgatgc accaataaag cggacttcag gctcaagcca gacattaaat ttttcaccca
     3541 ctttttgtct tacatggtgt gccagttgca ccacatcttc gctttttgca ttgtcttcat
     3601 taatgagaac taacgcctgt tgacggtgca ccgcagcccc acccatttgc atccctttta
     3661 gctggcactg atcgataagc caacctgctg ccagttttac tgaaccatcc gcctgggggt
     3721 aatttggtgc tgttggaaat tgtgacagta atgctttagc cgtttcggca gatacaacag
     3781 ggtttttgaa gaaactaccg gcattgccat tcacttttgg gtcaggaagt ttggtggtgc
     3841 gcatatgaca caccgcatca aatacttgct gtggcgttac tgttgtagga tccagacgag
     3901 ttaagtcacc atacgttagt acaggttgcc actcttttgg cagacgcaga cctacggcta
     3961 caatggcgaa gcggtcctgg tattcatgtt taaaaatact gtcgcgatag ccaaaacggc
     4021 actctttggc agttaagcgc acttgcttgc ctgtcgccag ttcaacacaa tcaacataag
     4081 cgcaaactcg ctgtaattct acgccataag caccaatatt ctggataggt gatgagccga
     4141 cacaaccagg aattaacgcc agattttcca gacctggcat accttcctgc aaagtgtatt
     4201 ttaccaggcg atgccagttt tctccggctc ctacatgtaa ataccacgca tcaagttcat
     4261 catgaatttc gatacctttg atccggttga tgatcaccgt gccgcgatag tctttcagaa
     4321 aaagtacatt acttccttca cccagaataa gaacgggttg cccttctgcg gttgcatgct
     4381 gccaggcatt gagtagttgt tgttcgtctt cggcacatac aatgtgctga gcattatgat
     4441 caatgccaaa tgtgttccag ggttttaagg agtggttcat agctgctttc ctgatgcaaa
     4501 aacgaggcta gtttaccgta tccgtggggg gatggcttgt agtaatgacg acagggagag
     4561 tttgtagaaa cgcaaaaagg cccagtcttc cgactgggcc t
//
LOCUS       DACBVI010000242         4587 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.245, whole genome shotgun sequence.
ACCESSION   DACBVI010000242 DACBVI010000000
VERSION     DACBVI010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.245"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            226..1383
                     /gene="acrE"
                     /locus_tag="IU325_21920"
     CDS             226..1383
                     /gene="acrE"
                     /locus_tag="IU325_21920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HAP5811644.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLSRNFTEGSDVQAGQSLYQIDPATYQA
                     SYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESAHINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQSDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADSASK"
     gene            1395..4499
                     /gene="acrF"
                     /locus_tag="IU325_21925"
     CDS             1395..4499
                     /gene="acrF"
                     /locus_tag="IU325_21925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HAP5811645.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGVLAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERSGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTAHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASQLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
BASE COUNT         1114 a         1109 c         1200 g         1164 t
ORIGIN      
        1 aatttacgag gatttgattc tacctctttc aacccgcgtc aaaataaaac agtagaatat
       61 taatcttttt ttgtgtttat gtgccttgag acgccagtat tcataactat tccttacatc
      121 gacgaatgat aatttgtagg atagcgaact gtatttttct ttctgcgagt taacgcgttg
      181 cctttttggg taaataacgc gcttttggtt ttttgaggaa tagtaatgac gaaacatgcc
      241 aggtttttcc tcctgccctc ctttattctg atctccgcgg ctttaatcgc cggttgtaac
      301 gataagggag aagagaaagc tcacgtcggt gaaccgcagg ttaccgttca tattgtgaaa
      361 acggccccgt tagaagttaa gactgaatta ccaggccgca ccaatgctta ccgtatagcc
      421 gaagttcgcc cacaggttag cgggatcgta ctgagtcgca atttcactga aggcagcgat
      481 gtgcaagcag gccagtctct gtaccagatc gatcccgcga cctatcaggc aagttatgac
      541 agcgcgaaag gcgaactggc gaaaagtgaa gccgccgccg ccatcgcgca tttgacggta
      601 aaacgttacg ttccgctcgt gggtacgaaa tacatcagcc agcaggagta cgaccaggcc
      661 attgctgatg ctcgccaggc cgatgccgcc gtgattgccg caaaagccac agtcgaaagc
      721 gctcacatca atcttgctta taccaaagtg actgcgccaa ttagcggacg tatcggcaaa
      781 tcgactgtga ccgaaggcgc tcttgtcact aatgggcaaa cgactgaact ggcgactgtc
      841 cagcagctcg atcctatcta cgttgatgtg acccaatcca gcaacgattt tatgaggctg
      901 aagcaatccg tagagcaagg aaatttgcat aaggaaaacg ccaccagcaa cgtagagttg
      961 gtcatggaga acggtcaaac ctatcccctg aaaggtacgc tgcaattctc cgatgttacc
     1021 gttgatgaaa gcaccggctc cataacccta cgtgctgtct tccctaaccc gcaacatacg
     1081 cttttacctg gtatgtttgt gcgcgcacgg attgatgaag gcgtccaatc tgatgccatt
     1141 cttatcccgc aacaaggcgt tagccgcaca ccgcgtggtg atgcaaccgt gctgattgtt
     1201 aacgataaaa gtcaggttga agcgcgccct gtcgttgcca gccaggcgat tggcgataaa
     1261 tggttgatta gcgaagggct gaaatctggc gatcaagtca ttgtcagcgg cctgcaaaaa
     1321 gcgcgtccgg gagagcaggt taaagccact accgataccc ccgcagattc tgcatcgaag
     1381 taaggcaatc tgacatggca aactttttta ttcgacgacc gatatttgca tgggtgctgg
     1441 ccattattct gatgatggcg ggtgtactgg cgatcctaca attgcccgtc gcccaatacc
     1501 caaccattgc gccgcctgca gtttctgttt cggcaaacta tccgggcgct gatgcgcaga
     1561 ccgtgcagga tacggtgacg caggttatcg aacagaatat gaatggtatc gataacctga
     1621 tgtatatgtc ctccaccagc gattctgccg gtagcgtgac aattaccctt accttccagt
     1681 ccgggaccga tccagatatc gcgcaagtgc aggtgcagaa caaactccag ctcgccacgc
     1741 cgttgctgcc gcaggaagta cagcagcagg ggatcagtgt tgaaaagtcc agtagcagct
     1801 atttgatggt ggcgggcttt gtctctgata acccagacac cacacaggac gatatctcgg
     1861 actatgtggc ctctaacgtt aaagatacgc ttagccgtct gaatggcgtc ggtgacgtac
     1921 agcttttcgg cgcacagtat gcgatgcgta tctggttgga cgccgatctg ctaaacaaat
     1981 ataaactgac gcctgttgat gtgattaacc agttgaaggt acagaacgat cagatcgctg
     2041 ccggacagtt gggcggaacg ccagcgttac cagggcaaca gttgaacgcc tcgattattg
     2101 ctcagacgcg gttaaaaaat ccggaagaat tcggcaaagt gaccttgcgc gtaaacagtg
     2161 acggctcggt ggtacgcctg aaagatgtcg cacgggttga acttggcggt gaaaactata
     2221 acgttatcgc tcgcatcaac ggtaaaccgg cggcgggcct ggggattaag ctggcaaccg
     2281 gcgcgaatgc tctcgatacc gcgaaagcca ttaaggcaaa actggcggaa ttacagccat
     2341 tcttcccgca gggaatgaag gttctctacc cttatgacac cacgccattc gtccagcttt
     2401 ctattcacga agtggtaaaa acgctgttcg aagctattat gctggtgttc ctggtgatgt
     2461 atctgttctt gcagaatatg cgagcaacgc tgatccccac cattgcggta cccgtggtgt
     2521 tgttagggac gtttgccatc ctcgccgctt ttggttactc catcaacaca ctaacgatgt
     2581 tcgggatggt gcttgccatc gggctgctcg tcgatgatgc gatagtggtg gtggagaacg
     2641 tcgagcgcgt gatgatggag gataagctcc cgccaaaaga agcgacggaa aaatcaatgt
     2701 cgcaaatcca gggcgcactg gtgggtatcg caatggtgct gtcagcggta tttattccga
     2761 tggcattctt cggcggttct actggggcaa tttatcgcca gttctctatc accatcgttt
     2821 cggcaatggc gctttctgtt ctggtggcat tgattcttac cccagcgtta tgtgcaacac
     2881 tgctcaaacc cgtctctgct gagcatcacg aaaataaggg cggtttcttc ggctggttta
     2941 ataccacctt cgatcatagc gttaaccact acaccaacag cgtcggcaaa atcctcggtt
     3001 ccacaggacg atatttactg atctatgcac taattgttgc tggaatggtg gtgttgtttt
     3061 tacgtcttcc gtcttccttc ttacctgaag aggatcaggg tgtctttctg actatgattc
     3121 agttacccgc tggcgcgacg caagagcgaa cgcaaaaagt gttggatcaa gtgacggatt
     3181 actatctgaa gaacgagaaa gcgaacgtag aaagtgtctt tacggttaac ggctttagct
     3241 tcagcggcca ggcacaaaac gccggtatgg ctttcgtcag tctgaaaccg tgggaagagc
     3301 gtagtggtga cgaaaacagt gcggaagcgg taatccatcg tgccaaaatg gaattgggca
     3361 agatccgcga cggttttgtc attccattca atatgccagc cattgttgaa ctgggcacgg
     3421 caacgggttt cgactttgag ttaattgatc aggctgggct gggtcatgat gccctgaccc
     3481 aggcccgtaa ccagttgctt ggcatggcgg cgcaacatcc tgccagctta gtcagcgtgc
     3541 gtcctaatgg tctggaagac accgcgcagt ttaaactgga agttgaccag gaaaaggcgc
     3601 aggcattagg tgtttcactt tctgacatca atcagaccat ttcaacggcg ttaggtggga
     3661 cttacgttaa cgacttcatt gaccgtggtc gcgtgaaaaa ggtgtatgtt caggcggatg
     3721 ccaaattccg tatgctgcca gaagatgtcg ataaacttta tgtccgcagc gccaacggcg
     3781 aaatggtgcc attctcggcc tttaccactg cacattgggt gtatggctct ccgcgactgg
     3841 agcgctacaa cggtctgccg tcaatggaga ttcaggggga agccgcgcca ggaaccagtt
     3901 ccggcgatgc catggcgttg atggaaaacc ttgcatcaca attaccagct ggcattggtt
     3961 atgactggac gggtatgtca tatcaggaac gcttatcggg aaaccaggct cccgctctgg
     4021 tagcaatttc ctttgtggtt gttttcctgt gccttgctgc actctatgaa agctggtcaa
     4081 ttcctgtctc ggttatgttg gtcgtgccgt tagggattgt cggcgtgcta ctggcggcga
     4141 cactctttaa tcaaaaaaat gacgtctact ttatggtggg cttgctaacg acaattggct
     4201 tgtcggctaa aaacgctatt ttgattgttg agttcgctaa agatctcatg gagaaagagg
     4261 gtaaaggtgt tgttgaagcg acactgatgg cagtacgtat gcgcctgcgt cctatcctga
     4321 tgacctctct cgcctttatt ctcggcgtat taccgctagc tatcagtaac ggtgccggca
     4381 gtggcgcgca gaacgcagtg ggtatcgggg taatgggcgg gatggtttct gcaacgttgc
     4441 tggcaatctt cttcgtgccg gtgttctttg tggtgatccg ccgttgcttt aaaggataaa
     4501 tcagaaacat aaaggcactt tcgggtgcct ttattatttt cagtgaaacc cataaaaatt
     4561 aataagatat tcttcttctc actcttt
//
LOCUS       DACBVI010000243         4547 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.246, whole genome shotgun sequence.
ACCESSION   DACBVI010000243 DACBVI010000000
VERSION     DACBVI010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.246"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..301)
                     /gene="chbB"
                     /locus_tag="IU325_21930"
     CDS             complement(<1..301)
                     /gene="chbB"
                     /locus_tag="IU325_21930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HAP5811646.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAI"
     gene            complement(600..938)
                     /gene="osmE"
                     /locus_tag="IU325_21935"
     CDS             complement(600..938)
                     /gene="osmE"
                     /locus_tag="IU325_21935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HAP5811647.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1140..1967
                     /gene="nadE"
                     /locus_tag="IU325_21940"
     CDS             1140..1967
                     /gene="nadE"
                     /locus_tag="IU325_21940"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP5811648.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2197..3084
                     /gene="cho"
                     /locus_tag="IU325_21945"
     CDS             2197..3084
                     /gene="cho"
                     /locus_tag="IU325_21945"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HAP5811649.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3044..3619)
                     /gene="ves"
                     /locus_tag="IU325_21950"
     CDS             complement(3044..3619)
                     /gene="ves"
                     /locus_tag="IU325_21950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HAP5811650.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3822..4307)
                     /locus_tag="IU325_21955"
     CDS             complement(3822..4307)
                     /locus_tag="IU325_21955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HAP5811651.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
BASE COUNT         1136 a         1107 c         1098 g         1206 t
ORIGIN      
        1 taatcgctgc aaccgcagcc ttaagcacgc ctaaaccatc gactttgcca taaagcagcg
       61 agtcaattac ttcaaccggt ttgttgggta acaaacgctg gatttcgggc aacatataag
      121 caatctgcgg ccctaataac acgacatcgg cattctgacc tttttcacca gccagtgttt
      181 ccggaaatgc ttcaataatg accggaactt catatttttc tgcctgtgcg cgcatttttg
      241 ataccagtaa agaggtagac atgcccgcag aacaaaacag ataaatgtgt ttcttttcca
      301 taaaactgcc ctcatcgacg attatctgtc agccagacac tccgcaagcc ttaacctgct
      361 tccatgctct gggtaacttg cgaaaccaac atgacttttt ttgtggctga aaggagtata
      421 cggtatagac ctgagcaata gtatttcttt gaccttataa attgtctctc cttgacctgc
      481 ggttatgacc ctcttcacat ttcgggcaaa taattcgcga agataattaa gaactcaggc
      541 caaaaaaaac cggcgcaatg gccggtttcc gttgtaactc aagctttcag gcgaagtgat
      601 tacttcgcag cctgtggatc agtgtcgtat tcagcacagg tctgataacc ggagttgatg
      661 acatgtccgg tatcatctaa cgcgacaaag taggtttctg ctttaccatc acgttgaccc
      721 aggatgtagg tctggcaagt accacgagca tggatcatgc tcacttcaga cgaaggttta
      781 cccgcaattt gtgcaacctg cgcccggctc atgccttttt tgacgtcttt caccacaggc
      841 tgtacaaact ggtctttggt acgatcataa gccgtacaac ccgccagcat ggttaatacc
      901 gccgctgcac tcagaattcc tgccatattc ttgttcatat tccgtcctct tgtttatcag
      961 cgtgttagat aagcctggaa tacattgggc gctttttcaa gcccgtgaac gaaacggctc
     1021 cgctttcaga ggattcctgt atgacgtttt aaccaccatt caacccgctg tcgcttgtcg
     1081 tttcagtagc aacgggttag ctttaaggaa gttttgtctt ttctgtctgg aggggttcaa
     1141 tgacattgca acaacaaata ataaaggcgc tgggcgcaaa accgcagatt aatgctgaag
     1201 aggaaattcg tcgtagtgtc gattttctga aaagctacct gcaaacttat ccgttcatta
     1261 aatcactggt gctcgggatc agcggcggtc aggactccac gcttgccgga aagctgtgcc
     1321 agatggcgat taatgagctg cgccaggaaa ccggcaacga atcgctgcaa tttattgccg
     1381 tacgcctgcc ctatggtgtt caggccgacg aacaagattg ccaggatgcc attgccttta
     1441 ttcaaccgga tcgcgtatta accgttaata tcaagggcgc ggtattggcc agcgaacagg
     1501 cgctgcgcga agcgggcatt gaactgagcg attttgttcg tggcaatgaa aaagcgcgtg
     1561 agcggatgaa agcacaatat agcattgcgg gtatgaccag cggtgtcgtg gtgggcaccg
     1621 atcatgcagc agaagccatt accggattct tcactaaata tggtgacggc ggtacggaca
     1681 ttaatccact gtatcgtctc aacaaacgtc agggtaaaca gttactggca gcattaggtt
     1741 gcccggaaca cctttataag aaagccccaa ctgccgatct ggaagatgat cgcccttctc
     1801 tgccagatga agtggcactc ggcgtgacct atgacaatat cgacgactat ctggaaggga
     1861 aaaacgtacc tgaacaggtc gccagaacaa tagagaactg gtatctgaaa actgaacata
     1921 aacgccgtcc gccaattacc gttttcgatg atttctggaa aaagtaataa tttgcacata
     1981 ttggtttgtg cgaaaaagag tactttgttc acgccggatg cggcgtgaac gccttattcg
     2041 ccccacaaaa ctatgcaaat tcaatatatt gcatgagtcg aataggcctg acaggcgtag
     2101 cacgtcagac ggtgtaacct ttgtcatcga cccgcctctt ttttaatcgc tccccgcctg
     2161 ttacactgga tagataacca gcattcggag tcaacagtgg tacggcgttt aacttctccg
     2221 cggctcgaat ttgaagctgc ggcaatttat gaatatcccg aacatttacg ttcattcctt
     2281 aatgacttac ccacccgacc aggggtgtat ctgtttcatg gcgaaagtga caccatgccg
     2341 ctctatatcg gcaaaagcgt taatatccgc agtcgggtgc tttctcattt acgcaccccg
     2401 gatgaagccg ccatgctacg gcaatcccgg cgaattagct ggatctgcac cgcaggcgaa
     2461 atcggcgctc tgctccttga agcgcgatta atcaaagaac aacaaccgct gtttaataaa
     2521 cggttgcgcc gcaatcgcca gctctgcgcc ctgcaattaa atgaaaagcg tgttgatgtg
     2581 gtgtatgcca aagaggtgga tttttcacga gcccccaacc tgtttggcct gtttgccaat
     2641 aggcgcgcag ctttgcaagc attgcagacc atcgctgatg aacaaaaact ttgctatggc
     2701 ctgctgggac tggaaccgtt aagccgcggg cgtgcttgtt ttcgttctgc attaaaacgt
     2761 tgcgctgggg cgtgttgcgg taaagagagc catgaggaac atgcgctacg tttgcgccag
     2821 tctctggagc gtttgcgggt ggtgtgttgg ccttggcaag gggcggtggc gctgaaagaa
     2881 cagcacccgg aaatgactca atatcatatt attcaaaact ggctgtggct gggagcggtt
     2941 aattcgctgg aagaagcgac aacgttaatt cggacacccg ccgggtttga tcacgacggt
     3001 tataaaattc tttgtaagcc gctgctttcc ggtaactatg aaattactga acttgatccg
     3061 gcgaatgacc agcgagccag ttgatttcac tgaacaacag tttaccctgc ggttgtaaaa
     3121 gccgcagggt gtgcctgcca tcgaaccagc acgcgccctg gtcggtagtg agcagtttgt
     3181 cgcccagttg ccacgcgccg ttaattacaa acaccacccc gccgcgtgag ccaaaggtag
     3241 taaacgtacg ttcggcaatt ctgactttcg ccttacagac atccaggcgt gtcatgatgt
     3301 tgaaatccat cgacatctgc cctgccgtca gtttcgcttt taccacctgg tccgccgcaa
     3361 aggcaaaagg ctgtaacggt tttaaggtat ggttaaagcg gtctgcgctt tcaaggaaca
     3421 tctcgccgcc ttccagcaac gtcactatcc tttccatacc tggaaacaaa gaaaactccc
     3481 cattcgccgc gatggatgca atgctggcac gccagtaaaa atcacgtttt gccggtggga
     3541 aagtgcaaat ttcgcgcgtt tcaccggcag catttcgcca cagattcacc gacattttac
     3601 gcatatcaaa gtattccata ctcgctcccg gcccgttctt ttcattgtta tcgttcaggt
     3661 aaccgtggat ggttatcggc aactcgggtg atttacgtta gtggcgatca ggcttttttt
     3721 gcatctgttt gagaacaaaa aagaaaaccg ccgatcctgt ccaccgcatt actgcaaggt
     3781 agtggacaag accggcggtc ttaagttttt tggctgaaag attattcagc agttgcaggc
     3841 attttacctt ttgccgctgg acgttctgtc agacgcttct caaaattagc attaaattgc
     3901 tttttctgtt ccggcgtcag gatgttgtaa attttgttct gggtttccat gtgcgccagc
     3961 atgttagctt tgcgctgttc ttccattttt gcgatctgcg cttcagcttt tgctttatcg
     4021 aaggtatcgc tggcaatgat gtcatgcatt gcgcggcgtt cttccagcgg cggacgtttc
     4081 atctggtcac gctggccttt catgatttcg cggatctgct gtttctgcgc gtcggtcagg
     4141 ttcaggtctt tgaacatcat gtcctgatgc ggaccgaact tgcctttgtg gtgcatcatc
     4201 ggcttcgcgt cagccggtgc tgcggtagtg gtgtctgcgg catgggccag gttagccgcg
     4261 ccaagagcca gggtagaggc aacaaacagt gcagttaatt tacgcatatt ctatatcctt
     4321 cctttcagtt atttattacg gctttcttaa gaagcgtgcc gtgttgacga gattaacttt
     4381 actgacttta gcgtcaatta atcagagcaa cggtaaaaca atgaaagtgt aaaaaacact
     4441 tttgcgccaa ttatggagaa aaaaagaaaa tttgatggag agtgatgagg gaatattaca
     4501 acacgatgat ttagcagaga ttatgaagaa ctataccgga tgactgg
//
LOCUS       DACBVI010000244         4460 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.247, whole genome shotgun sequence.
ACCESSION   DACBVI010000244 DACBVI010000000
VERSION     DACBVI010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.247"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..153
                     /locus_tag="IU325_21960"
     CDS             <1..153
                     /locus_tag="IU325_21960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP5811652.1"
                     /translation="DIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQ
                     NSFKHT"
     gene            309..1844
                     /gene="gadC"
                     /locus_tag="IU325_21965"
     CDS             309..1844
                     /gene="gadC"
                     /locus_tag="IU325_21965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HAP5811653.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAVVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKGNTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1975..3294
                     /locus_tag="IU325_21970"
     CDS             1975..3294
                     /locus_tag="IU325_21970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HAP5811654.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPASRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPEVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3671..>4460
                     /gene="dosC"
                     /locus_tag="IU325_21975"
     CDS             3671..>4460
                     /gene="dosC"
                     /locus_tag="IU325_21975"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HAP5811655.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRS"
BASE COUNT         1140 a         1054 c         1066 g         1200 t
ORIGIN      
        1 gacatcgtgg tgatgcgcat tatgtgtcgt cgcggcttcg aaatggactt tgctgaactg
       61 ttgctggaag actacaaagc ctccctgaaa tatctcagcg accacccgaa actgcagggt
      121 attgcccagc agaacagctt taaacatacc tgataacgtt taacggtaac ggtgtcccga
      181 aacacacccg tttcgggaca atttccaaag tctgttcact ggcattagca acggaaaata
      241 ttgttctgaa tacgtttcag aacaaaacag gtgcggttcc gacaggaata ccgttttagg
      301 gggataatat ggctacatca gtacagacag gtaaagctaa gcagctcaca ttactcggat
      361 tttttgccat aacggcatcg atggtaatgg ctgtttatga ataccctacc ttcgcaacat
      421 cgggcttttc attagtcttc ttcctgctat taggcgggat tttatggttt attcccgtgg
      481 gactttgtgc tgcggaaatg gctaccgtcg acggctggga agaaggtggt gtcttcgcct
      541 gggtatcaaa tactctgggg ccgagatggg gatttgcagc gatctcattt ggctatctgc
      601 aaatcgccat tggttttatt ccgatgctct atttcgtgtt aggggcactc tcctacatcc
      661 tgaaatggcc agcgctgaat gaagacccca ttaccaaaac tattgcagca ctcatcattc
      721 tttgggcgct ggcattaacg cagtttggtg gcacgaaata cacggcgcga attgctaaag
      781 ttggcttctt cgccggtatc ctgttacctg catttatttt gatcgcatta gcggctattt
      841 atctgcactc cggtgccccc gttgctatcg aaatggattc gaagaccttc ttccctgact
      901 tctctaaagt gggcaccctg gttgtatttg ttgccttcat tttgagttat atgggggtag
      961 aagcatctgc aacccacgtc aatgaaatga gcaacccagg gcgcgactat ccgttggcta
     1021 tgttattgct gatggtggcg gcaatctgct taagctctgt tggcggtttg tctattgcga
     1081 tggtcattcc gggtaatgaa atcaacctct ccgcaggggt aatgcaaacc tttaccgttc
     1141 tgatgtccca tgtggcaccg gaaattgagt ggacggttcg cgtgatctcc gcactgctgt
     1201 tgctgggtgt tctggcggaa atcgcttcct ggattgttgg tccttctcgc gggatgtatg
     1261 tcacagcgca gaaaaacctg ctgccagcag cattcgctaa aatgaacaaa aatggcgtac
     1321 cggtaacgct ggtcatttcg cagctggtga ttacttctat cgcgttgatc atcctcacca
     1381 ataccggtgg cggtaacaac atgtccttcc tgatcgcact ggcgctgacg gtggtgattt
     1441 atctgtgtgc ttatttcatg ctgtttattg gctacattgt gttggttctt aaacatcctg
     1501 acttaaaacg cacatttaat atccctggtg gtaaaggggt gaaactggtc gtggctgttg
     1561 tcggtctgct gacttcaatt atggcgttta ttgtttcctt cctgccgccg gataacatcc
     1621 agggtgactc taccgatatg tatgttgaat tactggtcgt gagtttcctg gtggtacttg
     1681 ccctgccctt tattctctat gctgttcatg atcgtaaagg caaagggaat accggcgtca
     1741 ctctggagcc aatcaacagt cagaacgcac caaaaggtca cttcttcctg cacccgcgtg
     1801 cacgttcacc acactatatt gtgatgaatg acaagaaaca ctaaccatgt ctgaatggtt
     1861 ataagataag ggagcgataa ttcatcgctc cctttttcgt gcttgctgtc gtattgactt
     1921 aaccgggata agtacgagaa tgagcgcaca tctgtttacc ggaaaccagc acatatggat
     1981 atctgctccc gaaacgagaa attagcgatt agaagaccag cgatactggt tgcactggca
     2041 cttttactgt gtagttgtaa aagcacgcct ccagagtcca tggtgacacc accagcgggt
     2101 tcaaagccac cagccacgac gcaacaatcg tcacaaccga tgcgtggcat ctggctggct
     2161 acggtgtctc gcctcgactg gccaccggtt tcctcggtta acattagtaa ccccaccagc
     2221 cgggcccgtg tacaacaaca ggcgatgatc gacaaactgg atcatctgca acgtctcggc
     2281 ataaacacgg tctttttcca ggtcaagccg gacggtaccg ccctgtggcc atcaaaaatt
     2341 ttgccgtggt ccgatcttat gaccggaaag attggtgaaa atccaggtta cgatccgctg
     2401 caatttatgc tcgacgaagc ccacaagcgt gggatgaaag tacacgcctg gtttaacccc
     2461 tatcgcgtat cggttaatac gaagcccggt actatcaggg aactgaatag cactctgtct
     2521 caacaaccag cgagcgtcta tgtgcaacac cgcgactgga tcagaacgtc cggcgatcgc
     2581 tttgtcctcg acccgggcat ccctgaagtt caggactgga tcacatcaat agtcgcggaa
     2641 gtggtttccc gctatccggt agatggcgtg cagtttgacg actatttcta tactgagtca
     2701 ccagcttcac ggctaaatga taacgaaacg taccgcaaat acggaggcgc atttgcgtca
     2761 aaagcagact ggcggcgcaa caatactcag cagttaattg cgaaggtatc gcacaccatt
     2821 aaaagcatta agccggaagt cgaatttggc gttagcccgg caggcgtgtg gcgtaaccga
     2881 tcacacgatc cgctcggttc cgatacccga ggcgcggcag cctatgacga atcctacgct
     2941 gacacccgtc gatgggtgga acaaggattg ctggattaca ttgctcccca aatttactgg
     3001 ccgttctcac ggagtgccgc acgttatgac gttttggcaa aatggtgggc agatgtcgtt
     3061 aaaccgacca ggacccgcct gtatatcggt atcgccttct ataaagtggg tgaaccttca
     3121 aagatagagc cagactggat gattaacggc ggcgtaccgg aactgaaaaa acagctcgat
     3181 cttaacgatg ccgtgccaga aattagcggc acaatcttgt tccgtgagga ctatctgaat
     3241 aaaccgcaga ctcaacaagc ggttagctat ctgcaaagtc gttggggcag ttaaactccc
     3301 attcatactt tgtgacaagc cggtggttta cccaccggct tgctaccctc ttgaaatatc
     3361 ccactagtaa tcatgcttac ttaagtcaaa ttaactacgc aattaaccac acttagttaa
     3421 acagtcataa atcccctaat aaaaatgtat gaacatgaaa aaacatacga tattcgctgt
     3481 caccgaaaaa taattacgat gggctatact cttctgaaga acagtagcgt agattgaaac
     3541 tgtcctaagt cgcttatttc ttttcagtat atcttcatat ttcaggagaa tatggtgcag
     3601 ttagtcgatc tcgcacgttg cgtttcgttt tgattccgtc tgctttccca gccttataag
     3661 ggtgtgaatt atggagatgt attttaaaag aatgaaagat gagtggaccg ggcttgtcga
     3721 acaagcagat ccgctcattc gtgctaaagc cgcggaaatt gccgttgcgc atgcccatta
     3781 tctgagtatt gagttttacc gaattgtccg tatcgacccg catgccgaag aattcttgag
     3841 taatgaacaa gttgagcggc agttgaaaag tgcgatggaa cgctggatta ttaacgtgct
     3901 ttctgcccag gtggacgatg tcgaaaggct aatacaaatc cagcataccg ttgcggaagt
     3961 gcatgcccgc ataggaattc cggtagaaat tgtcgagatg gggtttcggg tgctgaaaaa
     4021 aatcctctat ccagtcatct tctcttcgga ttattccgcc gcagaaaaac ttcaggtcta
     4081 ccatttctct attaacagta ttgatatcgc tatggaagtg atgacccgcg cgtttacctt
     4141 tagtgacagt agtgcctcaa aggaagatga aaactatcgt atcttctcgt tactggaaaa
     4201 cgccgaagaa gaaaaagaac ggcaaatagc ctcaatactt tcatgggaaa tagatattat
     4261 ctataaagtc ctgctggatt ctgatttagg cagtagtttg cctttaagcc aggctgattt
     4321 tggcctgtgg tttaaccata aaggtcgaca ttattttagc ggcatagccg aagtgggcca
     4381 tatctcccgt ctgattcagg atttcgacgg tattttcaat caaaccatgc gtaacaccag
     4441 gaatttgaat aacagaagtt
//
LOCUS       DACBVI010000245         4402 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.248, whole genome shotgun sequence.
ACCESSION   DACBVI010000245 DACBVI010000000
VERSION     DACBVI010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.248"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..473
                     /locus_tag="IU325_21980"
     CDS             <1..473
                     /locus_tag="IU325_21980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP5811656.1"
                     /translation="VDTGTLTIKGNIAESPCKFEAGGDSVSINMPTVPTTVFEGKAKY
                     STYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGNDKAIASSNDTVGYYLYLGDN
                     SDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQSGDVLSSLVMRVAQD"
     gene            554..1228
                     /locus_tag="IU325_21985"
     CDS             554..1228
                     /locus_tag="IU325_21985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAP5811657.1"
                     /translation="MKGLLALLISSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSINVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            1244..3724
                     /locus_tag="IU325_21990"
     CDS             1244..3724
                     /locus_tag="IU325_21990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAP5811658.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCISREIIKRLGINTDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNSNPGGWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSEGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRTDGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAINKQQGLSCTITFGKEIDESRNYICQ"
     gene            3740..>4402
                     /locus_tag="IU325_21995"
     CDS             3740..>4402
                     /locus_tag="IU325_21995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HAP5811659.1"
                     /translation="MKIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTNGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLALSYNPITTTCKPDDLLITLESTPVSQLPATGN
                     KAT"
BASE COUNT         1290 a          834 c         1070 g         1208 t
ORIGIN      
        1 acgttgatac cggaacatta accattaaag ggaatattgc agaatctccg tgtaaattcg
       61 aagcgggtgg cgattcagta agtattaata tgccaactgt accaaccact gtctttgaag
      121 gtaaagctaa atactcgacc tatgatgatg cagtcggtgt aaccagcagc atgttaaaaa
      181 ttagctgtcc gaaagaagtt gctggtgtaa aactctcgtt gattaccaac gataaaataa
      241 ccggaaacga taaggcgata gccagtagca acgataccgt ggggtactat ctctatttag
      301 gtgataacag cgatgtcctg gatgtttctg caccttttaa cattgagagt tataaaacag
      361 cggaaggtca atatgctatt ccgtttaaag caaaatacct gaagctgaca gataattcag
      421 tgcaatcagg tgatgtgtta tcttctctgg ttatgcgtgt ggcgcaggat taataaataa
      481 aacttcaagt ttctccaggt aataaacatg gccgctatcc ccatggcggc cttttaattt
      541 aagaggcatt aagatgaaag gattattagc tttactcatt tcttctatgg tccttcctgc
      601 acatgccggt attgttatct acgggacgcg tattatttac ccggcagaaa ataaagaagt
      661 gatggtgcag ttgatgaatc agggaaaccg ttcttcgctg ctgcaggcgt ggattgatga
      721 tggcgatacg tcgttaccac cagaaaaaat tcaggttcct ttcatgttaa cgccaccagt
      781 ggcaaaaata ggggcaaatt ccgggcagca agtaaaaatc aaaattatgc cgaataaact
      841 gcccactaat aaagagagca ttttttatct gaatgttctg gatattccac caaatagtcc
      901 agagcaagag ggcaagaatg cactgaaatt tgcaatgcaa aacagaatta aattgtttta
      961 ccggcctgcg ggtattgcgc cagtaaataa agcgacattt aaaaagttac tggtaaatcg
     1021 cagtggcaat ggattggtga taaaaaatga ctcagcaaat tgggtaacga tttcggatgt
     1081 caaagctaat aatgtcaaag tcaattatga aactattatg attgccccct tagaaagtca
     1141 gagtattaat gtcaaaagta ataatgcgaa taactggtat ctgaccatta tcgatgacca
     1201 tggcaactat attagtgaca aaatttaatt gcaggagctg cctatgttga gaatgacccc
     1261 acttgcatca gcaatcgtag cgttattgct cggcattgaa gcttatgcag ctgaagaaac
     1321 ctttgatacc cattttatga taggtggaat gaaagaccag caggttgcaa atattcgtct
     1381 tgatgataat caacccttgc cggggcagta tgacatcgat atttatgtca ataagcaatg
     1441 gcgcgggaaa tatgagatca ttgttaaaga caacccgcaa gaaacatgta tatcaaggga
     1501 aattatcaag cggttaggca ttaataccga taactttgcc agcggtaagc aatgtttaac
     1561 atttgagcaa cttgttcagg gtgggagcta tacctgggat atcggagttt ttcgtctcga
     1621 tttcagtgtc ccgcaggcgt gggtggaaga actggaaagt ggctatgttc caccggaaaa
     1681 ctgggagcgg ggtattaatg cgttttatac ctcttattat gtgagtcagt attacagcga
     1741 ctataaagcg tcgggtaata gcaagagtac atatgtacgt tttaacagcg ggttaaactt
     1801 actggggtgg caactgcatt ctgatgccag tttcagtaaa acaaatagca atccaggggg
     1861 gtggaaaagc aataccctgt atctggaacg tggatttgcc caacttctcg gcacgcttcg
     1921 cgtgggtgat atgtacacat caagcgatat ttttgattct gttcgcttca gcggtgtgcg
     1981 gttgtttcgt gatatgcaga tgttgcctaa ctcgaaacag aattttactc cacgggtgca
     2041 ggggattgct cagagtaacg cgctggtaac tattgaacag aacggttttg tggtttatca
     2101 gaaagaggtt cctcctggcc cgtttgcgat tacagatttg cagttggccg gtggtggagc
     2161 agatcttgat gtcagcgtga aagaggcgga cggttcggta accacctatc tggtgcctta
     2221 tgcagcggtg ccgaatatgc tgcaacctgg cgtgtcgaaa tatgattttg cggcgggtcg
     2281 tagccatatt gaaggggcga gcaaacaaag tgattttgtc caggcgggtt atcagtatgg
     2341 ttttaataac ttattgacgc tgtatggcgg atcgatggtc gcgaataatt attacgcgtt
     2401 cactttgggg actggctgga atacacgcat tggtgccatt tccgtcgatg ccacgaagtc
     2461 gcatagtaaa caagacaacg gcgatgtgtt tgacgggcaa agttatcaaa ttgcctacaa
     2521 caaatttgtg agccaaacgt cgacgcgttt tggtctggcg gcctggcgtt attcgtcgcg
     2581 tgattaccgg acatttaacg atcatgtttg ggcaaacaat aaagataatt atcgccgtga
     2641 tgaaaacgat atctatgaca ttgccgatta ttaccagaac gattttggcc gtaaaaatag
     2701 cttttctgcc aatatgagcc agtcattgcc agaaggctgg ggttctgtgt ccttaagtac
     2761 gttatggcga gattactggg ggcgtagcgg cagcagtaag gattatcagt tgagttattc
     2821 caacaacctg cgacggataa gctataccct cgcggcaagc caggcttatg acgagaatca
     2881 tcatgaagag aaacgtttta atatttttat atcgattccc tttgattggg gtgatgacgt
     2941 tacgacgcct cgtcggcaaa tatatatgtc taactcaacg acgtttgatg atcagggttt
     3001 tgcctcaaat aatacgggat tatcgggaac cgttggaagc cgggatcaat ttaactatgg
     3061 ggtcaacctg agtcatcagc atcagggaaa tgaaacgaca gctggagcga atttaacctg
     3121 gaacgcgccg gttgcgacag tgaatggcag ttatagtcag tcgagtactt atcgacaggc
     3181 tggagccagt gtttcagggg gcattgtcgc ctggtcggag ggcgttaatc tggcgaaccg
     3241 tctttccgaa acgtttgcag tgatgaatgc gccaggaatt aaagatgctt atgtcaatgg
     3301 gcaaaaatat cgcacaacaa accgtaatgg agtggtggta tacgacggaa tgacacctta
     3361 tcgggaaaat cacctgatgc tggatgtgtc gcaaagcgat agcgaagcag aattgcgcgg
     3421 taaccggaaa attgccgcac cttatcgcgg cgcggttgta ctggttaatt ttgataccga
     3481 tcagcgcaag ccctggttta taaaagcgtt aagaacggat gggcaaccat taacgtttgg
     3541 ttatgaagtc aatgatatcc atggtcataa tattggcgtt gtcggccagg gaagccagtt
     3601 atttattcgc accaatgaag taccgccgtc ggttaatgtg gcaattaata agcaacaagg
     3661 actttcatgc acaatcacct tcggtaaaga gattgatgaa agtagaaatt atatttgcca
     3721 gtaatagtca ggtggttcta tgaaaattcg cataatgcta tttatattaa tgatgatggt
     3781 tatgccagtg agctatgcag catgttatag tgagttatct gttcagcaca atttggttgt
     3841 tcagggggat tttgcactta ctcaaacaca aatggcgacc tatgagcata attttaatga
     3901 ttcgtcatgc gtgagtacaa atactatcac ccctatgagc ccgtcggata ttattgttgg
     3961 actttataac gataccatta aattaaattt acattttgaa tggaccaata aaaataacat
     4021 cacgttgtca aataatcaga ccagtttcac caatggttat tcggtaacgg tgacacctgc
     4081 agccagtaat gcaaaagtga atgtttctgc ggggggcggc ggttcagtga tgattaatgg
     4141 agttgcgaca ttatccagtg cttcatcatc gacacgcggg agtgccgcag tacaatttct
     4201 actgtgttta ttaggtggca agtcatggga tgcatgtgta aatagctaca gaaatgcatt
     4261 ggcacaaaat gcaggtgtct attcctttaa tctggcattg tcatataacc cgataacaac
     4321 gacctgcaaa ccggacgatt tattaattac tttagagagt actcccgttt cacaattacc
     4381 agccacaggt aacaaagcaa ca
//
LOCUS       DACBVI010000246         4345 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.249, whole genome shotgun sequence.
ACCESSION   DACBVI010000246 DACBVI010000000
VERSION     DACBVI010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.249"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..200)
                     /locus_tag="IU325_22000"
     CDS             complement(<1..200)
                     /locus_tag="IU325_22000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811660.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDLALP"
     gene            429..1037
                     /locus_tag="IU325_22005"
     CDS             429..1037
                     /locus_tag="IU325_22005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HAP5811661.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1135..2526
                     /locus_tag="IU325_22010"
     CDS             1135..2526
                     /locus_tag="IU325_22010"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HAP5811662.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LENEQRFLEMLLK"
     gene            2523..>4345
                     /gene="selB"
                     /locus_tag="IU325_22015"
     CDS             2523..>4345
                     /gene="selB"
                     /locus_tag="IU325_22015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HAP5811663.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLTDAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQVIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDA"
BASE COUNT          972 a         1117 c         1285 g          971 t
ORIGIN      
        1 ggcagggcaa ggtcggtttc gccgggaagg accttcgcac cgagcagcgg attgaccgga
       61 tggccggacg tcaccccgcc ggggcacacc gaacaggcca cgccggtggg ggcaccaata
      121 cgtaccccgg cagaaccctg aacaatgcta ccgccatact gcgtcatgtc gccctgacgc
      181 gccgccggtt ttccgctcat ctgctcatcc ttgttttatc gggttatttg tctgctgaca
      241 ggcgaaaccc tacagattta taagaaagcc ttaagtgaga tatctgtcag ataagaacaa
      301 ttaggaaaat atttatatca ccatgataaa tatgtgattt attgggaagt aacaaaccat
      361 caacattttc tgcccttttt cccatcacca ggccatactg atatttttcc tcgcacaaag
      421 gactcggcat gaaactcgtc ggtagctaca ccagcccgtt tgtacgcaaa ctttctattc
      481 tgttgttaga aaagggcata actttcgaat ttattaatga actgccctat aacgcggaca
      541 acggcgtggc gcaatttaac ccgttaggaa aagtgccggt gctggtgacc gaagagggcg
      601 aatgctggtt tgattcgccg atcatcgctg aatatattga attaatgaat gtcgctccgg
      661 cgatgttgcc gcgcgatccg ctggagtcgt tgcgggtgcg caaaattgag gcgctggcgg
      721 atggcattat ggatgccgga ctggtatcgg tgcgtgaaca ggcgcgtccg gcggcgcagc
      781 agtctgaaga tgaattgtta cgccagcggg agaaaatcaa ccgcagtctg gatgtgctgg
      841 aaggatatct ggtcgatggc acactcaaaa ccgatacggt caatctggcg actatcgcca
      901 ttgcctgtgc cgtcggatat ctcaatttcc gccgcgttgc gccaggttgg tgtgtcgacc
      961 gcccacactt agtcaaactg gtcgaaaacc tgtttagccg cgaaagtttt gctcgcaccg
     1021 aaccgccaaa ggcttgatgc gcgatatgtc ctcctgaccc atctcacgtt acaatccgtg
     1081 gttatgttaa acgcccttct ccgtgtgaga gggccttgat cagccaggtt tcctatgaca
     1141 accgaaacgc gttccctcta tagtcaactt ccggctattg atcgcttatt gcgcgatagc
     1201 tccttccttt ctttgcgtga tacttatggt cacacccgcg tggtggaatt gctgcgtcag
     1261 atgcttgacg aagcgcgaga agtgattcgt gacagccaga cgctgcccgc atggtgtgaa
     1321 aactgggcgc aagaagtcga tgcccgactg acgaaggagg cgcagagcgc gctgcgtccg
     1381 gtgatcaacc tgacgggaac cgtgctgcat accaatcttg ggcgagcttt acaggcagaa
     1441 gccgcagtgg aagccgttgc gcaggctatg cgttcgccag tgaccctcga gtacgatctg
     1501 gacgacgccg gacgcggaca tcgcgatcgg gcgctggcgc agctgctgtg ccgcattacg
     1561 ggagcggaag atgcctgtat cgttaataac aatgcggcgg cggtgttatt gatgttggcg
     1621 gccactgcca gcgggaaaga ggtggtggta tctcgcggcg aactggtgga gattggcggc
     1681 gcgtttcgta ttcctgatgt catgcgtcag gcaggctgca ccctacacga agtggggacc
     1741 accaaccgca cgcacgctaa tgattatcgt caggcggtga atgaaaatac cgcgctgttg
     1801 atgaaagtac ataccagtaa ctacagcatt cagggattca ccaaagcgat agatgaagcg
     1861 gaactggtgg cgctcggcaa agagctggat gttcccgtag tgactgattt aggcagtggc
     1921 tcgctggttg atcttagcca gtacggtttg ccgaaagagc caatgccgca ggagttgatt
     1981 gcggcgggtg tcagtctggt gagcttctcc ggcgacaagt tgttaggcgg gccgcaggca
     2041 ggaattattg ttggtaaaaa agagatgatc gcccgcctgc aaagccaccc gctgaagcgt
     2101 gcattacgcg cggataaaat gaccctcgcg gcgctggaag ccacgttgcg tctttattta
     2161 caccctgaag cactgagtga aaaattaccg accctgcgcc tgcttacccg cagcgcagag
     2221 gtcattcaaa tccaggcaca acgtttacag gccccccttg ccgcacatta cggcgcggag
     2281 tttgcggtac aggttatgcc atgtctttcg cagattggca gtggttcgct gccggttgat
     2341 cgcctgccga gcgcggcatt aacgtttaca ccccatgatg gacgcggtag ccaccttgag
     2401 tcattagccg cccgctggcg tgaattgcca gtgccggtga ttggtcgtat ttatgacgga
     2461 cgattgtggc tggatttacg ctgccttgaa aatgagcaac ggtttttgga gatgttgttg
     2521 aaatgattat tgcgactgcc ggacacgttg accacggcaa aacaacctta ttgcaggcga
     2581 ttactggcgt aaatgccgac cgtctgccgg aagaaaaaaa gcgcggcatg accatcgatc
     2641 tcggttatgc ctactggccg cagccggatg gtcgcgtgcc tggttttatc gacgtacccg
     2701 gtcatgaaaa gtttctttcc aacatgctgg cgggcgttgg tggtatcgat cacgcgctgt
     2761 tggtggtggc gtgcgatgac ggcgtgatgg ctcagactcg tgagcatctg gcgattttgc
     2821 agctgaccgg taacccgatg ctgacagtgg cgctgaccaa agccgatcgc gtggacgaag
     2881 cgcgtgttga tgaggttgaa cgccaggtaa aggaggtgct gcgggaatac ggttttgctg
     2941 aggcaaaact gtttatcacc gcagcaaccg aaggtcgggg aattgatgcc ctgcgcgagc
     3001 atttgcttca gttaccagaa cgtgaacacg ccagccaaca tagtttccgc ctcgcgatcg
     3061 accgtgcatt taccgtaaaa ggtgccgggc tggtcgtcac cggtacggcg ttaagcgggg
     3121 aagtgaaggt aggcgattca ctctggctga ctggtgtaaa taaaccgatg cgtgtacgtg
     3181 cgctgcatgc acaaaaccag ccaacagaaa ccgcccatgc cgggcagcgt atcgcgctta
     3241 acatcgcggg tgatgcggaa aaagagcaga ttaaccgtgg cgactggctg cttaccgatg
     3301 cgccgccgga gccgttcaca cgggtgattg tcgagcttca aacccataca ccgctgaccc
     3361 agtggcagcc gctgcatatt caccacgccg ccagccacgt cacgggacgc gtttcgctgc
     3421 tggaagataa ccttgccgag ctggtattcg acacgccgct gtggctggcg gataacgatc
     3481 gcttagtgct gcgcgatatc tcggcccgca acacgctggc gggggcgcgt gtggtgatgc
     3541 ttaacccgcc gcgtcgcggt aagcgtaagc cggaatatct gcaatggctg gcgtctcttg
     3601 cacgagcgca gagcgatgcc gatgcgttat ctgttcatct ggaacgcgga gcggttaacc
     3661 ttgcagattt cgcctgggca cggcagctca acggcgaagg gatgcgcgaa ctgctgcaac
     3721 agcctggcta tattcaggct ggttatagct tgttgaatgc gccggttgcc gcccgctggc
     3781 agcggaaaat tctcgacaca ttagcgactt atcatgagca acatcgcgat gaacctggtc
     3841 ctgggcgcga acgtctgcga cgtatggcgt tgccaatgga agatgaagcg ctggtactgt
     3901 tgctgattga aaagatgcgc gaaagcggcg acatcctcag tcatcacggt tggctgcatc
     3961 tgccggatca taaagcgggc ttcagcgaag agcagcaggt catctggcaa aaagcagagc
     4021 cgctgtttgg tgacgaaccg tggtgggtgc gtgacctggc aaaagagacg ggaaccgacg
     4081 agcaggcaat gcgcctgacg ctacgccagg cggcgcagca ggggataatt accgcgatcg
     4141 ttaaagatcg ttattaccgt aacgatcgga ttgtcgagtt tgccaatatg atccgcgatc
     4201 tcgatcagga gtgtggttca acctgcgcgg cggatttccg cgatcgctta ggtgtaggcc
     4261 gaaagctggc aattcagatt ttggaatatt ttgaccgcat tggctttacg cgtcgtcgtg
     4321 gaaatgatca tttattgcgc gacgc
//
LOCUS       DACBVI010000247         4344 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.250, whole genome shotgun sequence.
ACCESSION   DACBVI010000247 DACBVI010000000
VERSION     DACBVI010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.250"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            121..399
                     /locus_tag="IU325_22020"
     CDS             121..399
                     /locus_tag="IU325_22020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811664.1"
                     /translation="MAHDTKLHNSDDSAVFASRCGRRFHAFKSDWFQHPPCTEEQAEW
                     IIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            401..1450
                     /locus_tag="IU325_22025"
     CDS             401..1450
                     /locus_tag="IU325_22025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAP5811665.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRLLVEPEPKSMRN
                     LPSGVVPAARQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWAVRNQVTDALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQQNPPQEKTVVSIAVDPESPESFMNRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            1463..1819
                     /locus_tag="IU325_22030"
     CDS             1463..1819
                     /locus_tag="IU325_22030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAP5811666.1"
                     /translation="MLIDLVLPYQPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            1834..2655
                     /locus_tag="IU325_22035"
     CDS             1834..2655
                     /locus_tag="IU325_22035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAP5811667.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNKVQYPDGKPPVWAKCTKGVYSSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            2799..2874
                     /locus_tag="IU325_22040"
     tRNA            2799..2874
                     /locus_tag="IU325_22040"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2832..2834,aa:Ile,seq:cat)
     gene            2975..3048
                     /locus_tag="IU325_22045"
     tRNA            2975..3048
                     /locus_tag="IU325_22045"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3009..3011,aa:Arg,seq:tct)
     gene            3551..3682
                     /locus_tag="IU325_22050"
     CDS             3551..3682
                     /locus_tag="IU325_22050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 domain-containing protein"
                     /protein_id="HAP5811668.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(3963..4298)
                     /gene="iraM"
                     /locus_tag="IU325_22055"
     CDS             complement(3963..4298)
                     /gene="iraM"
                     /locus_tag="IU325_22055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HAP5811669.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKVKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCILQIC
                     PYGLKQQSP"
BASE COUNT         1087 a          937 c         1164 g         1156 t
ORIGIN      
        1 aacaatctgt tgtttgactt ttattccatt aatgtagggg ctttacactg gaaccagttt
       61 atttatactt tatacgccag cctgaacaac tggcacctgc tgcgccagca gagacaaccg
      121 atggcgcacg ataccaaatt acacaattct gatgattctg ccgtctttgc cagcaggtgc
      181 ggacggcgtt ttcatgcatt caaatcggac tggttccagc atccaccatg cactgaagag
      241 caggctgaat ggataattca gtgttaccgc aggcgcggat acgaggttaa aaaagccctt
      301 agcctcgact accgtcactg gataatctca gtcagattgc cttactccga acgcccaccg
      361 cgtccgtccc gcacattcca gcaacgcatc tggaggtaac gtgcgggtat tacttcgacc
      421 tgttctggta ccggaactcg ggctggtgat cgttaagccg ggccgtgaat ccatgccggt
      481 attccacaat acccggttac tggtggagcc ggaaccgaaa agcatgcgta atctgccgtc
      541 cggggtcgtt cctgccgctc gccagccgct ggtggaagac aaaacattgc tgccgttttt
      601 cagtaacgca cgggtgattc gtgctgctgg tggtgctggt gcattgtctg actggctgtt
      661 gcgccatatt aaatcctgcc agtggccaca cggcgattat catcacagcg aaaccgtcat
      721 tcaccgttat ggtaccggcg caatggtgtt gtgctggcac tgcgacaacc agttgcgtga
      781 ccagacctcc gaatcacttg agcaacttgc tcaccaaaac ttgtcagcat ggatgattga
      841 cgtcattcgt cacgcaatga atggcacaca ggagcgggaa ttatctctgg ctgaattatc
      901 ctggtgggcg gtccgcaatc aggtgacgga cgcgctaccg gaagcggtat tacgtcgttc
      961 gctggggttg cgtgcggaaa aaatccgctc aatgtaccgt gaaagcgaca tcgtaccggg
     1021 agagcagacc gccaccagca tactgaaaca gcgcacaaaa aatcttgcgc cgctgcctca
     1081 cgcccaccag caacagaacc caccacagga aaagacggtg gtcagcattg ccgttgatcc
     1141 tgagtctccg gaatctttca tgaatcgacc taaacgtcgc cgctgggtta acgagaaata
     1201 cacacgctgg gtgaagacac agccgtgtgc gtgttgtggt aagccagccg acgatcccca
     1261 tcacctgatt ggtcatggtc agggcggaat ggggacaaaa tctcacgata ttttcacgct
     1321 accgctgtgt cgggagcatc acaacgagct tcatgcggat ccgctggcgt tcgaagaaaa
     1381 gcatggttct caggttgatt taatttttcg ttttcttgat cacgcctttg caactggcgt
     1441 gcttgggtaa aagaggtgac tgatgctcat agatttggtt ttaccttacc aaccgacggt
     1501 gaacacttac tggcgacgcc gtggcagcac atattttatc tcggaggagg gaaagcgtta
     1561 tcgccgggct gtggcgctta ttgttcgcca gcagcggctg aaattaagcc tgtccggaag
     1621 gctggcgata aaggtgattg cagagccacc ggataagcgt cgtcgcgacc tggacaatat
     1681 cctgaaagca ccgctggatg cgctgacgca tgcgggagtg ttaatggacg atgagcagtt
     1741 tgatgaaatc aatatcgttc gtggtcagcc agtatctggt ggacgtctgg gggtgaagat
     1801 ttaccccata atgcattaag agcaggtcaa aaaatgaaac tggaagattt accgaaatac
     1861 tactccccaa aatcccctgg cctgaccgat gcatcggcct caacgtcaaa agatgcgctg
     1921 agtatcactg atgtgatggc cgcgcagggc atgacacaga atcgggctga gatgggtttt
     1981 tctgcgttcc tggggaaaat gggcatcagt atgaatgaca gggcgcgggc aacagaatta
     2041 ctggcagatt atgcactcag tcggtgcgat cgtgtggcgg cgttgagaaa acttccggca
     2101 gaaataaaac cggtagtgat gcgcattatg gcttcgtacg cttttgagga ttatgcccgt
     2161 agcgcagcga gtaaaaagca gtgcccttgt tgctatgggg aaaaatttat tgaaagcgta
     2221 gtttttacaa acaaggtcca gtatccggat ggtaagccgc cggtatgggc aaagtgtacg
     2281 aaaggtgtgt attcgtctta ctgggaagaa tggaaaaaag tcagggaggt ggtaaaagtt
     2341 gcctgtccgg agtgtggcgg aaagggtgag gtttccaccg cctgtaagga ttgccgtggg
     2401 cgtggtgtcg ccattcatcg tgaagagtcg gtaaaacgtg gtatgcctgt tatcagagac
     2461 tgccagcgtt gtggtggtcg tggctatgaa agactaccat caacggaggc atttaatgct
     2521 atatgcgagg tgacaaacca gataacacgc gcgtcatggg aaaaaacagt taagaaattc
     2581 tatgatgcgc tggtgacccg gtttgatatt gaagaagcat gggctgagcg gcagttaaaa
     2641 aaggtaacta ggtaacaagg ttgatttttc cggaatctgt ggtaaattcg tcataacaat
     2701 gggcttttta tgcctgacgt tagaagagtt tctacaaccc gccgccgagc gggtttttta
     2761 ttgcggaatt aattacggac cgttattatt ctgctcccgg ccctttagct cagtggtgag
     2821 agcgagcgac tcataatcgc caggtcgctg gttcaaatcc agcaagggcc accatcacaa
     2881 accgccatta gcttatcagg aagagcagac gacacgataa cagggttgtt ggtgcggggg
     2941 cgggtccccg atggcggtcc attatcggta ttcagcgttg ttagctcagc cggacagagc
     3001 aattgccttc taagcaatcg gtcactggtt cgaatccagt acagcgcgct atattcattc
     3061 ttccagattc cttccggcag agccttatac tgaaatatac ctggctcagg atattgttga
     3121 aaatatttta tgtttgtcaa aaataaaagt tctgttaagt attgattgag tgtttgttat
     3181 acggtctaat ggttttttca gcattaaata tttatcattc atatggtgtg ggtagagtga
     3241 atattgatga ggcgtcgggg tgtttcatcc ttaggcagcg tattgatata gtcaatgcag
     3301 gacgagcaaa ggccttcagc cgtttgacag ttttgttctg tactcctgat cgtctttcgg
     3361 gaagagacgt tattattctg aatagtgatg ctatacagag ggtttgcgat gagttcatgg
     3421 ttgctaattc agaattattt gctcttgttc aggagtacaa cagaatagcc agtacctgtg
     3481 gtatggatga acttcggatt actcatctgg ggtagataca tatctggatt atcacttgtt
     3541 acggtaaaaa gtgattgctt actgtttttg tgagtggcat tgcagcagcc ggataatgtc
     3601 agtgctggct gacggtgtgc tggtggcggg tgtggtggtt gttgctttcc cgttgctgaa
     3661 aaagaaaacg ccagactgtt agccgggtat cagttagcgg gagaaatttt taaatacttc
     3721 acaattcagg cggttgactg ttgtctggtt tgcggggagt ttgttaaaag aaactggcat
     3781 ggtgaatccc cctgtgcgga ggggcaatca gcgagtaggt atatgggata atcgcggatt
     3841 caggtgctgg tactgaattc accgggaggc acccggcacc atgcaatggc acatagcgcc
     3901 actctccagc ccctctccgg aggggctgtt tatattgatt ttgtcagatg tgagtaaact
     3961 ccttatggac tttgttgttt tagtccataa ggacatattt gcagaatgca acggttatta
     4021 aagcattcat tcaatacgtt atctgtattt gtagggcatt cctggctgtt tttgattaaa
     4081 ttccagaatg ttttattgaa tggtactacg ttgtaaatgg ttacaggtag cactttgtta
     4141 ttgagcatga tacctgtgtg agtcagtgta aatatacttt caggaggtaa gaaagcatcc
     4201 gattgatacc agattattaa ttttacttta ctccatatga ctgaaaaaga tattccgcat
     4261 gatggctgga taactgtatc aatcacaatc cacttcattt agtttccttg tttatgcctt
     4321 gctggtgatg ttctgaaaag tata
//
LOCUS       DACBVI010000248         4334 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.251, whole genome shotgun sequence.
ACCESSION   DACBVI010000248 DACBVI010000000
VERSION     DACBVI010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.251"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(348..863)
                     /gene="pgpA"
                     /locus_tag="IU325_22060"
     CDS             complement(348..863)
                     /gene="pgpA"
                     /locus_tag="IU325_22060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP5811670.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGIL"
     gene            complement(841..1818)
                     /gene="thiL"
                     /locus_tag="IU325_22065"
     CDS             complement(841..1818)
                     /gene="thiL"
                     /locus_tag="IU325_22065"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HAP5811671.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRAGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADVKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGASIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(1896..2315)
                     /gene="nusB"
                     /locus_tag="IU325_22070"
     CDS             complement(1896..2315)
                     /gene="nusB"
                     /locus_tag="IU325_22070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP5811672.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(2335..2805)
                     /gene="ribE"
                     /locus_tag="IU325_22075"
     CDS             complement(2335..2805)
                     /gene="ribE"
                     /locus_tag="IU325_22075"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAP5811673.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(2894..3997)
                     /gene="ribD"
                     /locus_tag="IU325_22080"
     CDS             complement(2894..3997)
                     /gene="ribD"
                     /locus_tag="IU325_22080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HAP5811674.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMESGESQWITSPQARLDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQGL
                     YPQHNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     ILPGLEKLADALQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(4001..4324)
                     /gene="nrdR"
                     /locus_tag="IU325_22085"
     CDS             complement(4001..4324)
                     /gene="nrdR"
                     /locus_tag="IU325_22085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HAP5811675.1"
                     /translation="MAELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEM
                     AINHIKSQLRATGEREVPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEI
                     ARLED"
BASE COUNT         1054 a         1211 c         1058 g         1011 t
ORIGIN      
        1 ttggttaccc gtgaaattca agatgaaata aaaccaaaga ttcaagacaa aatagatgaa
       61 ctttctaaaa atttattcaa cactataaac caaaccatta aggataactt tgatgatatt
      121 tttcggcaac agtcagagaa tatgagcaat tattatgagt tcgtcgactg acgatatttg
      181 agtgtacgtg aattatgagt gacaaagata caccttaaaa tatcgccgta taatatattc
      241 ggtgatgtcg ggttttactt taacagagtg ccccaaaatt ggggcactgt tcatttataa
      301 atcaaaccgg gtggtctgga atcgcctaca cctcccggtc aatggcatta aagaataccc
      361 agcggccagt gatgaccgat aaagtacagg atgcctgcgg aaattacccc ggcgacaata
      421 tcgtcgatca tgatccccat gccgccatgt acattgcggt caaaccagcg gatcggccac
      481 ggtttccaca tatccaggat acggaaaatc acaaacccgg cggcgaccca ctgccagtca
      541 ttggtcggca gcgccatgag cgtgatccac ataccaataa attcgtccca gacaatgctg
      601 ccgtgatcgt gcacgcccat gtctttcgcc gtttgatgac aaagatagac gccgatacag
      661 atccccagca tcaccaccag cgaatagagc tgccagggca aaaaggtcat cagataccag
      721 aacggaatcg ccgccagcga tcccatcgtg ccaggaacga ttgggcttaa tccacttccg
      781 aatccgacag caagcagatg ccacggatta ctcatcttca ggcgactttt cgcgacatct
      841 ttatggcgtg gcaaaatggt catatccttt ccagtcgaac gtgacaggtt cgccgtcacg
      901 aataaaacaa agcccttcga tatcggcggt catttgcccg atacaggtaa acggtacgcc
      961 cagatgtccg agagccacat ccagcgcgcc acggttcagt tccggcaccg tgaaacacaa
     1021 ctcgtaatct tcaccgccag agagcgccca gcgcagcgcc tgttccggtt caacatggcg
     1081 agaaagcgca tcagaaaacg gcagcaatgc caggtcaata cttgcgccgc agtcgctggc
     1141 tttcacgata tgcccgagat cggaaatcag accgtcagag agatcgatag ctgaatttgc
     1201 cagatcgcgc agtgcctgtc cctgtaaaat acgcggagat ggacggagat gacgtttgat
     1261 caagtagtcc gcatctttaa catcggcaac ctgcaaacgg ttttgcaaaa tcgccagccc
     1321 ggcggcgcta tcgcccggtg taccggtcac atagatccag tcacccggtt tcgccccagc
     1381 gcgcgttaag gctcgtccca tcggaacaaa gccgtggata cccagcgtca ttgataatgg
     1441 cccacgcgtg gtatcgccgc caatgagttg catatcgtaa taattgagaa gatcaaacaa
     1501 actgtcgctg aaggactcaa gccacgcttc gtcgacgtcc ggtaaggtta atgccagcgt
     1561 cagccaggcc ggatcggccc ccatcgcagc cagatcgctt aggttcaccg ccagtgcttt
     1621 ataagccaga tcagcaggat cgatatcagg gaggaagtgg ttgcccgcca ccagcgtatc
     1681 agtgctgatc gccagggtct gtttctcggg gatattgaga agtgcgcaat cgtcgccaat
     1741 gcccagttcg acatcaagac gagaacttct tacacggtca aaataacggg caatcaggga
     1801 gaactcgcca catgccatac gttatgcctc agcaggtaaa aaagaaaagg ccggcgatcg
     1861 cggaacggtc ttccgtgaat ctaccggcct ggatatcact ttttgttagg gcgaatcaca
     1921 ggtgctgctt tatcgagtac gccgttgacg aacttatggc tgtcttctgc gccgaacgat
     1981 ttcgccagtt cgatcgcttc gttaatggcc actttgtatg gcacatcgct acgtttagac
     2041 agctcgtaca gcgcaatgcg cagtactgct ttttctacct ggcccagttc ttccagcagg
     2101 cgggacaggt atggcttcat cagtccgtcg aggtatgcgg tgttagtcgc caccccggcc
     2161 agcagctcac ggaagtacag gacgtcaacg tcttttacat cctgttcagc caggaactgg
     2221 tattcaacat cagcgatgtc gttctgggac aactgccagg agtagagcgc ctggacggca
     2281 cactcacgag cgcggcgacg agcagcaggt ttcacggatt tccccttact aatttcaggc
     2341 cttgatggct ttcaatacat taatcatttc aagcgcggtc agtgcagctt ctgcaccttt
     2401 gttgccagct ttggtgccag cacgttcgat cgcttgttca atgctttcag tggtcagaac
     2461 cccaaaagca accggaattt cgctgtcctg ggcaacatgc gccaggccgt tgcttgcacc
     2521 accagcgaca tattcaaagt gggcagtgcc accacgaata accgtaccca gcgcaatcac
     2581 cgcgtcgtat ttaccggttt tagccagtgc acccgccgcc agcggcagct cataggcacc
     2641 aggcacccaa acaacggtaa tgttttcatc ttttacctga ccgatacgtt tcagtgcatc
     2701 aattgcacct tccagcaggc tgtcattgat aaagttgttg aaacgcgcga tggtgatggc
     2761 gacgcgagcg tccggggtag caacgttagc ttcaataatg ttcattttct tccttcgggt
     2821 tcgagtatgg ccccgcaggg gggcggattt tagcataata tttcgtgcgc tgcttccctt
     2881 tcgagccggg agatcatgca cccactaaat gcaggcaaac atccgggcct acatgacgta
     2941 tctctttgaa tttaaattgg agggcgtcag ctaatttctc aagccctggt agtatgcata
     3001 atccacgggc gtcgctgcct aatagtttag gtgcgatata gacaatcagc tcatcgacta
     3061 aacccgcctg cagcaatgcg ccagcgagcg ttggccccgc ttccacccag atgctgttaa
     3121 tctgctgttt acccagttgc atcattagta caaccagatc cagatgacct ttatgctctg
     3181 gaatcagcaa ggtacgcacc gtttccggcc actcacgaga atcttcctgc gtacgcgcga
     3241 accaggtttc gccgggctgc tgcacaatgc gatgttccgg cgtcacgcga ttttggctat
     3301 caatcacaat acgtaccggc tggcggagat tgtgttgcgg gtagagcccc tgagtttgtt
     3361 catccagttc agaccaacgc accgttaagg ccggatcatc cgccagcacc gtggcactgc
     3421 tggttaaaat ggcatgactt tgtgcgcgca gtcgttgtac atcgagtcgc gcctgaggcg
     3481 aagtgatcca ctggctttcg ccactctcca tcgccgtgcg accatcaagc gatgcgccaa
     3541 gttttaactg aatataagga aagccggtgc gcatccgctt gagaaagcct ttattcaatt
     3601 gctcggcttc actcatcatc aggccgtggc tgacgtcaat accagcctgt tgcaggcgat
     3661 atagccctcg cccagctacc tgcgggttag gatcttgcat cgaggcaacc acgcgcgcca
     3721 cgcccgcagc aattaatgca tcacaacacg gtggcgtacg accatgatgg ctacagggtt
     3781 cgagtgtgac atacgccgtt gcgcctttgg ctttttcgcc agccatgcgc aacgcgtgta
     3841 cctcggcatg cggttcaccc gctcgttggt ggtaaccttc accgacaatt tcgccatctt
     3901 tgacaatgac gcacccgaca ttcgggttgg gatgcgtggt aaaacgtcct cgttgcgcca
     3961 gctttagcgc ccgcgccatg taatactcgt cctgcacggc ttagtcctcc aggcgcgcga
     4021 tctcttcgcc aaattctttg atatcttcga aactgcggta gacagaggca aaacggatat
     4081 aggcgacttt atcgagcttt ttcaattgct ccatcaccag attgccaatc atcttgctcg
     4141 gcacttcgcg ctcacccgtg gcgcgcagct gcgatttaat atgattgatt gccatttcga
     4201 catcatcgga actcaccgga cgtttttcca gcgcccgcag cattccgcta cgcaatttct
     4261 cttcattaaa cggttcacgc acgtcgttgc tttttacaac acgcggcata accagctccg
     4321 ccacttcaaa ggtg
//
LOCUS       DACBVI010000249         4159 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.252, whole genome shotgun sequence.
ACCESSION   DACBVI010000249 DACBVI010000000
VERSION     DACBVI010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.252"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            64..366
                     /locus_tag="IU325_22090"
     CDS             64..366
                     /locus_tag="IU325_22090"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP5811676.1"
                     /translation="MSRKTQRYSKKFKAEAVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLGTPGSRTVAELESEILQLRKALNEARLERDILKKATVDFAQESLKNTR"
     gene            402..1220
                     /locus_tag="IU325_22095"
     CDS             402..1220
                     /locus_tag="IU325_22095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP5811677.1"
                     /translation="MCQVFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRET
                     YGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNSNHNLPVAPNLLNQ
                     TFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFM
                     ALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSRKGNCYDNAPMESFWGTL
                     KNESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
     gene            1374..3371
                     /locus_tag="IU325_22100"
     CDS             1374..3371
                     /locus_tag="IU325_22100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HAP5811678.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVSLALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            3434..3847
                     /locus_tag="IU325_22105"
     CDS             3434..3847
                     /locus_tag="IU325_22105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HAP5811679.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLLKWSTKTGHRVRVFPVSIFRFVWG"
     gene            complement(3782..>4159)
                     /locus_tag="IU325_22110"
     CDS             complement(3782..>4159)
                     /locus_tag="IU325_22110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAP5811680.1"
                     /translation="TMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQ
                     SMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAITDYIVGYYSALRPHEYN
                     GGLPPNESENRYWKNSNAVASFC"
BASE COUNT         1013 a         1002 c         1051 g         1093 t
ORIGIN      
        1 gaggtagcct gagtttaacg gacactcctt cctgaaatag aatggcatca gaaggagcta
       61 ataatgagca gaaaaaccca acgttactct aaaaagttca aagccgaagc tgtcagaacg
      121 gttcttgaaa atcaactttc gatcagtgaa ggcgcttccc gattatctct tcctgaaggc
      181 actttaggac aatgggttac cgccgccaga aaagggctcg gtactcctgg ttcccgcacg
      241 gtggctgaac tggaatctga aattctgcaa ctgcgtaagg cgttaaatga agctcgcctt
      301 gagcgagata tattaaaaaa agcaactgta gattttgcac aggagtcgct gaaaaatacg
      361 cgttaatcga acaatggcga caacaatttc ccattgaagc gatgtgtcag gtatttggtg
      421 tatccaggag cggttattac aactgggtac agcatgaacc ctcagacaga aaacaaagtg
      481 atgagcggct aaaactggag attaaggtgg cacatatccg cactcgcgaa acatatggaa
      541 cccggcggct ccagacggag ctggcagaga atggcatcat cgttggtcgt gaccgactgg
      601 cacgtcttcg taaggagcta aggctacgct gtaagcagaa acgcaagttc agagcgacta
      661 cgaactcgaa ccacaatctg ccagttgcgc caaatctgct gaaccagacg ttcgctccta
      721 cagcaccaaa tcaggtctgg gtggcggacc tgacgtatgt tgccacacag gagggatggt
      781 tgtacctcgc tggcatcaaa gatgtttata cgtgcgaaat tgtcggctac gccatgggag
      841 agcgcatgac aaaagagctg acaggtaaag ccctgtttat ggcgctcagg agccagcgcc
      901 cacctgccgg gctaatccac cactctgatc gaggttcaca gtactgcgca tacgattacc
      961 gggtcataca ggagcagttt ggtctgaaaa catcaatgtc gcgtaaaggt aactgttacg
     1021 acaacgctcc gatggaaagc ttctggggaa cgctgaaaaa tgagagcctg agccactatc
     1081 gttttaataa ccgggatgaa gccatctcag taatacggga atacattgag attttctaca
     1141 atcgtcagcg tcgtcactct cgtctgggga atatctcccc ggcagccttc agggaaaaat
     1201 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
     1261 tcaacattcc accatgcatt ccgattaacg ccgcatagcc agttgaactt tgctactttg
     1321 tgagaggtag taccttctat ccagtgcgaa tttaattaat ggaataaatg attatgagtg
     1381 aaaatgatac aatcccaaag aagtctacaa gtcagattaa caaagcggta ttctttacat
     1441 ctgctttgct aattttcctt cttgtcgcct ttgccgccgt attcccggat gtcgccgaca
     1501 aaaattttaa actacttcag caacaaatct tcacgaatgc cagctggttc tacatccttg
     1561 ctgtggccct gattttactg agtgtcacgt tccttggact ctcacgctac ggtgatatca
     1621 agctgggccc ggaccatgcg cagcctgatt tcagctacca ctcctggttt gcgatgcttt
     1681 tttcggcagg gatggggatc ggcctgatgt tctttggcgt tgccgaacct gtaatgcatt
     1741 atctttcgcc acccgttggc actccagaaa ccgttgcggc agccaaggaa gcaatgcgtc
     1801 tgaccttttt ccactgggga ctgcacgcat gggcaattta tgccattgtg gcgctgattc
     1861 tggcgttctt cagttaccgt cacggtctgc ctttaactct gcgctccgca ctctatccca
     1921 ttattggcga tcgcatatac ggacctgtag gacatgcggt tgatattttc gctgttatag
     1981 gcacggtctt tggcgttgcg acatcactgg gttacggtgt tttgcaggtg aatgccggtt
     2041 tgaaccatct tttcggggtg cccatcaatg aaacggtgca ggtaattctg atcgtggtca
     2101 tcacggggtt agcgacgatt tcagtggtgt ccggtctgga taagggaata cgtatcctgt
     2161 ctgaactcaa tctgggtctg gctttgttgc tcctggcgct ggtcctgtgt ctgggaccaa
     2221 ccgtgcttct gctgaagtca tttgtggaaa atacgggcgg ttatctttcg gaactggtga
     2281 gtaaaacgtt caacctttac gcgtatgagc ccaagtcgag caactggctg gggggctgga
     2341 cattactgta ctggggatgg tggctttcat ggtcgccgtt tgtggggatg ttcatcgcac
     2401 gggtctcccg cgggcgaacc attcgcgagt ttgtcaccgg cgtgctgttt gttcccgcgg
     2461 gttttacgct aatgtggatg acggtgtttg gtaacagcgc gatctatctc attatgaacc
     2521 agggggccac agacctcgcc aatactgttc agcaggatgt gtcgctggcc ctgtttaatt
     2581 tcctggagca tttcccgttc tcttctgtgc tgtcattcat tgcaatggcg atggtcatcg
     2641 tcttctttgt aacgtctgct gattcggggg caatggttgt ggatactctg gcatcaggtg
     2701 gagtggcaaa cacacccgtc tggcagcgaa tattctgggc ctcgctcatg ggcattgttg
     2761 caattgcgct tctccttgcc ggagggctaa gtgcgctgca aacggtgaca atagcgagtg
     2821 cattgccctt ctcagtgatc ttactaatat ccatatacgg acttttaaaa gctttgcgcc
     2881 gggatttgac caagcgtgaa agcctgagca tggcgacaat tgctcctacg gctgcacgta
     2941 acccaattcc ttggcagaga aggttacgca atatcgcgta tctgccgaag cgatctcttg
     3001 tgaaacgttt tatggacgac gttatccagc ccgccatgac gctggttcag gaggaactga
     3061 acaagcaggg gacgataagc cacattagtg atgcagtcga cgatcgtatt cgtcttgaag
     3121 tcgatttggg caacgagctg aattttatat atgaagtgag gcttcgcggg tatatctcac
     3181 cgaccttcgc gctcgccgca atggataatg atgagcagca gactgaacaa catcgatatt
     3241 atcgcgctga ggtttatctc aaagaaggcg gtcaaaatta tgatgtgatg ggctggaacc
     3301 aggaacagct gattaatgac atactggacc agtacgaaaa acacctgcac ttcctgcacc
     3361 tggttcgtta atagcaacat gccgtcctgg gggcggcaat tattatctcg gccgcaatat
     3421 gagggaatgc agaatgattt cacgctggaa atggatgctg aagcagacaa ttaaaaaact
     3481 atggttcagg gcaacgttat tcgcaattgt cgcgataata acggcccttt tatcaattct
     3541 ttttaaatca atgatacctg agtcggtttc cgtgaaggtt ggtgcggaag cagtcgataa
     3601 cattctgaac atactggcat cgagtatgct ggcagtgacc acattttcgc tgagtatcat
     3661 ggtcacagcc tacggttcag ccactactaa tgtgactccc agagctacgc gtttagttgt
     3721 tgaagacgtc accacacaaa atgtactggc caccttcatc ggttcttttc tcttgaagtg
     3781 gtcaacaaaa actggccacc gcgttagagt ttttccagta tcgattttcc gattcgtttg
     3841 ggggtaaccc accgttatat tcgtgcggtc ttagtgcgct gtaatatcca acgatatagt
     3901 ccgttatggc gtgagctgcc tcgctgaagc ttacgtaacc caccaccggc atccattcgt
     3961 tcttcagact cctgaagaag cgttccattg ggctgttatc ccagcagttt ccgcgccggc
     4021 tcatactctg tctgatctgg tatcgccaca ataactgccg gaactgcctg ctcgtataat
     4081 gactgccctg atcgctgtgg aacatcaccc cgccgggctt accacgggtt tcccatgcca
     4141 tttccagcgc tttcatggt
//
LOCUS       DACBVI010000250         4118 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.253, whole genome shotgun sequence.
ACCESSION   DACBVI010000250 DACBVI010000000
VERSION     DACBVI010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.253"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(272..1942)
                     /gene="betA"
                     /locus_tag="IU325_22115"
     CDS             complement(272..1942)
                     /gene="betA"
                     /locus_tag="IU325_22115"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HAP5811681.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNITVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPHGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGSAE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTARYFVANGMPVRVKK"
     gene            complement(1956..3428)
                     /gene="betB"
                     /locus_tag="IU325_22120"
     CDS             complement(1956..3428)
                     /gene="betB"
                     /locus_tag="IU325_22120"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HAP5811682.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNNELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPSLEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTD
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQNNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3442..4029)
                     /gene="betI"
                     /locus_tag="IU325_22125"
     CDS             complement(3442..4029)
                     /gene="betI"
                     /locus_tag="IU325_22125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HAP5811683.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVDGNFDETQVS
                     NAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFHRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
BASE COUNT          877 a         1173 c         1102 g          966 t
ORIGIN      
        1 ttttcttcag ataaaataaa atctcaatca gatcgatatc ctgtttgatt tgttcactca
       61 taatatatcc agaaaatgaa atctgtcgca gataaatgaa ttaatagtct gtatcaggag
      121 tgttcgggtt aaatatcagc aaaaaaccag catccagaat actggatatg aagcatgaga
      181 gttacctcag tgttttatat aaggattcgg tcctcatagt agaacggtaa ctctcaatct
      241 aatcggttcc tgagtcagtt cacataacga ctcatttttt cactctcacc ggcatcccat
      301 ttgccacaaa atatcttgcc gtgctcctcg gcaacgcttc ttgcccgcga atcatatccg
      361 ctattttctc gccaatcata atcgtcgtgg cgttcaaatt gccggtgata atttgcggca
      421 taattgacgc atccaccacg cgcagacctt ccagcccgtg tacgcggcct tcgccgtcaa
      481 ccactgccat ctcgtcataa cccattttgc aggtaccgca cgggtggaag gcggtttcgg
      541 cgtggttacg cacgaactca tcgagctgct catccgtctg gcattcgaca ccggggctga
      601 tttcgcggcc acgatactga tccagcgccg gttgatgcat gatctcgcgg gtgatgcgaa
      661 ttgcgtcgcg gaactcctgc cagtcctgct cgtgcgacat gtagttaaac agaatcgctg
      721 gatgctggtg cgggtcgcgg gatttaatcc gcacatgccc acggcttggc gagcgcatcg
      781 atccgacgtg gcactggaag ccgtgctctt tcactgcgtt cgagccgtta tagttaatcg
      841 ccactggcag gaaatggtac tgaatattcg gccacgcaaa ttcctcacgg ctgcgaataa
      901 atccgcctgc ttcaaagtgg ttgctggcac caacgccggt gccgccaaac agccactccg
      961 caccgatttt cggctgattc caccactgca gggcagggta gagggaaacc ggttctttgc
     1021 actcatattg caggtacatc tccaggtgat cctgaagatt ttcgccgacg ccgggtaaat
     1081 catgcaccag cggaatatca aactccgcca gcagttcagc gctacccacg ccggaacgtt
     1141 gcaggatctg cggagaggcg atcgcacctg cacataacag cacctcttta ttcgctgtcg
     1201 cgcgggttgg aatggtgctg tcgccttcca gccactcgac gcccaccgcg cgtttgccgt
     1261 caaaaatgat gtgatcggtc atggcgtgag tacgaatggt caggttaggg cgcgatttgg
     1321 cctgatcgag atagccacgc gcggtgctgg cgcgacggcc atgcggcgtg acggtgcgat
     1381 ccatcgggcc aaaaccttcc tgctgatagc cgttgagatc gtccgtgcgc gggtagcctg
     1441 cctgcacgcc cgcttcaatc atcgcttcaa acagcggatt gacgccgggt ttggaggtag
     1501 tgacgctcac cgggccatcg ccaccgtggt agtcgttctc gcctacatcg cgagtctcgg
     1561 ccttgcggta gtagggcagg cagtcgagat aactccagtt ctccagaccg ggttcttgcg
     1621 cccagttatc gagatccagc gcgttaccac ggatgtaaca catgccgttg atcagtgatg
     1681 acccgccaag ccccttaccg cgtccgcact ccatgcgacg gttattcata aacggttcag
     1741 gttccgtttc ataggcccag ttgtagcgtt taccctgtaa cgggaatgcc agcgcggcgg
     1801 gcatctgggt gcggaagtca aagcgatagt ccgggccgcc tgcttcaagc agcagcacgg
     1861 ttatgttcgg atcttcagtc agacgggtag cgaggacgtt gccggctgag ccggcaccaa
     1921 taatgatgta gtcaaattgc aaataaacct cctggttaga atatggactg gaatttagcc
     1981 atctcaacct ggatggactt cacctgggtg taactctgga gcgtcatcac gccgttctcg
     2041 cgaccaatgc cggagtgttt gtagccgcca acgggcatct cagcagggga ttcgccccag
     2101 gtgttgatcc agcaaatacc cgcttccagc tgatgaatga cgcgatgcgc gcggttcagg
     2161 tccgccgtca cgatgcccgc cgccaggccg tagtcggtat cattggcgcg gcgaatcact
     2221 tcgtcctcag attcataagt gagaagcgac atcaccggac cgaagatctc ttcgcgcacg
     2281 atggtcattt cgtcgcggca atcggtgaac acggtcggtg caacccatgc gccgttatcg
     2341 aagccatcgc ctttcagtac atcgccgccg cacagtacgc gcgcgccttc ctctttgcct
     2401 ttggcgatgt aacgcagcac gttatcgcga tgcgggaagc tgaccagcgg gccaaagtta
     2461 ttttgcggat cgaaaacgtc gcccgcgcga atgcgctcaa cgcgcgccag aatcttttgt
     2521 tcaaatgcgg ctttgcattt cgccggaacg aagacgcggg tgccgttggt acacacctga
     2581 ccggagctga agaagttcgc catcatggcg atatcagcgg cgagatcgag atctgcatcg
     2641 tcgaaaacga tcagcggcga tttaccgccc agctccatgg tcacttcttt cagggaggaa
     2701 gctgccgagt tagccatcac ttttttgccg ctggcgacac cgccggtaaa tgacacttta
     2761 gcaatgcccg gatgatcggt cagatattgc ccggtctccg cgcccacgcc cggcaacacg
     2821 ttaaatacac cgtccggcag gcccgcttcg ctgtaaattt cagccagctt taacgcggta
     2881 agcggggtga cttcgctcgg tttgaagatc attgcattgc ctgccgccag cgccggggcg
     2941 gatttccaca gggcaatctg gatcgggtag ttccatgcgc caatccctgc cactacgccc
     3001 agcggttcgc ggcgggtata gacaaatgac gtctcacgca atgggatctg gctgccttcc
     3061 agcgacggga tcagcccggc gtagtactcg agcacgtccg cgccggtaac gatatcgacg
     3121 gttgaggttt ccgaatacgc ttttccggtg tcgagggttt ccagttttgc gagttcgtta
     3181 ttgcgttcac gcagaatatc aacggcccga cgcagaatac gcgagcgttc catagcggtc
     3241 atcgccgccc agattttttg cccctgttgg gcactcttca cggcgcgatc gacatcctca
     3301 cgcccggcgg cctgcacggt cgccagcacg ttaccgttgg ccgggttaat ggtctcgaag
     3361 gtgcgaccgc tggtggcgga ggtataacca ccatgtatat aaagctgctg ttctgccatt
     3421 cgggacatca cgtctcctcg gttaatcggt gggtaaatgc tgagtaataa agtgacgggt
     3481 cagcgagtgt gcgagaggtt tatccagcgg tttgccgctc agtgccgcgc gcagccataa
     3541 tccgtcaatc agcgcggcaa ggccgtagcc cgcttcctgt gcctgttggc gcggtaactc
     3601 gcggtgaaat tcgctcacca gattcgacaa caagcggcgg ctgctcacct gctgcaaacg
     3661 gtaaagcatt ggctgatgca tactgctggc ccaaaacgcc aaccaggctt tcatcgcagc
     3721 attactgacc tgcgtttcat cgaaattccc gtcaacaatc gcctgtaatc gctgctctgc
     3781 actgccctgc ggaagtgcat gtaatcgatt caaaaccgcg tcacgcagct gactggtgat
     3841 atcgcgcatg gttgcttcca gcagaccatt tttgtccctg aaatagtggc tgatgatccc
     3901 cgtagaaacg cctgcacggc gggcgatctg cgcgatcgtt gcatcgtgca tgcccacttc
     3961 atttattgct tccagcgtgg cgtcgatcag ttgtctgcgc cggatcgact gcatccccaa
     4021 tttgggcatt ttcgccactc cattcatcag cgttgtttat ctattaaagc ggttattgat
     4081 tggacgttca atataaaatg tgtcttaatt gttacgaa
//
LOCUS       DACBVI010000251         4114 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.254, whole genome shotgun sequence.
ACCESSION   DACBVI010000251 DACBVI010000000
VERSION     DACBVI010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.254"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(593..721)
                     /locus_tag="IU325_22130"
                     /pseudo
     CDS             complement(593..721)
                     /locus_tag="IU325_22130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014837283.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(694..852)
                     /locus_tag="IU325_22135"
     CDS             complement(694..852)
                     /locus_tag="IU325_22135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811684.1"
                     /translation="MMMSDTTLSRPEVVSGHTDVIYSTSVCHILAVRKSTLLPIDTII
                     RHQVSDIS"
     gene            complement(952..1128)
                     /locus_tag="IU325_22140"
     CDS             complement(952..1128)
                     /locus_tag="IU325_22140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HAP5811685.1"
                     /translation="MKSLTTETALDILITWLQDNIDCESGIIFDNDEDKTGSAALLPC
                     IEQAREGIRTLRQQ"
     gene            complement(1145..1555)
                     /locus_tag="IU325_22145"
                     /pseudo
     CDS             complement(1145..1555)
                     /locus_tag="IU325_22145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312567.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1640..2386)
                     /locus_tag="IU325_22150"
     CDS             complement(1640..2386)
                     /locus_tag="IU325_22150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP5811686.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTA
                     EELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQGNWLTLKQNIL
                     LTGATGSGKTFLACALGHNACRQGYKVYYYRLKALMEQCYQGHADGRYSKLLTRLNNS
                     DLLLLDDWGLEPLSSEQRSDLLEIVDLMYQRGSIIVVSQLPVENWYKMIGDSTHADAI
                     LDRLVHGSIKIELKGESMRKIQSPLTEGDQ"
     gene            complement(2401..3942)
                     /locus_tag="IU325_22155"
     CDS             complement(2401..3942)
                     /locus_tag="IU325_22155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /protein_id="HAP5811687.1"
                     /translation="MPTVPISMRKLKEILRLKYGVGLSHRQIGRSLAISPSVVSRYAN
                     RAAQLGIKQWPLPTGWDDTKLKHAFLQTQVKMKKHSLPDWATVHRELRNKCVTLQLLW
                     EEYCERNPGGFYSYNHYCRMYREWLKTTSPSMRQVHKAGEKLFVDYCGPTVGVTDPET
                     GEIRTAQVIVAVLGASSYTWAEATWSQQLEDWVMSHVRCFQWLGGVPELVVPDNLKSA
                     TSRACKYDPDVNPTYQQMLEHYNVAVLPARPRKPKDKAKAEVGVQVVERWIMARIRHE
                     IFYSLASLNQRIRELLERLNNKIMQKLGYSRAELFIQLDKPALKPLPEASYSYTLVKK
                     VRVHADYHVEIDKHYYSVPCSLLGQQLEAWISGELVRLFNQGQEVAVHPRKRTYGYST
                     RNEHMPEAHRQHATWTPERLLEWAGHIGSETHSYVLHILNSRPHPEQSYRFCLGLLNL
                     HKKYSKARLNAACARALKTKVWRLSGIKSILEKGLDKQPVQDPKPDLLSTMEHENVRG
                     SEYYH"
BASE COUNT         1039 a          980 c          972 g         1123 t
ORIGIN      
        1 aaccatagtt acacaggtaa tcatcattat tcgccaagat aataaatatt attcggtaga
       61 atagcgttct caggtggtca gtaaaattgt cttcaacaag atgtctgcgc atctcgtctg
      121 ctagccctac ataactgctc tcgaccgaaa ctatttattg aatgttcacc aaggtgggcg
      181 tggagacaaa atgacgtctt ttgatattag ctacctgagc cacatggtca aaacgttcaa
      241 gtagcatacc gaaaccaccc tgcccacgta atgtggacga tgcagcttcc agccagagca
      301 aacttgggac catcgactac aaaatgaaga atactgaact gcttttctgg caggcctata
      361 tactgcacgg atatgggtga tttcagacac aaaaaaagcc gctcttgagc gacttgattt
      421 gcatacggtg tggtgcgaag gccggactca aacatgcgat ttaacctcat gatttaaaat
      481 tgttaataat aaacaacacc atcctatacc aacacagata ccaacacgaa aaatcactgg
      541 ttttcggcat cgaaccagga caatcaggtc ctgattgaga taccgagctc tgttactcgt
      601 catcttcctc gacaggctcc attgcatcaa gtggtactcg caccatcgcg atagaaccaa
      661 tccggataaa agcatcttcc ccatctatat cggttaactg atatcgctta cttgatgccg
      721 gataattgtg tcgattggca acagtgtact ctttcgtaca gccagaatgt ggcagactga
      781 ggtggaatag ataacgtccg tatgcccgct gaccacctcc gggcgggaga gtgtggtatc
      841 tgacatcatc atttttcctt tctgtttata aatgaaaacg ccagccgtgt tcaggctgac
      901 gtcagggaag tgaaatcggg tgagtgatct tcactggttc tggtgcaaaa gttactgttg
      961 gcgcagggta cggataccct ccctggcctg ttcgatacag ggcaacagtg ctgccgaacc
     1021 tgttttatcc tcatcgttgt cgaagataat tcccgattcg cagtcgatat tgtcctgcag
     1081 ccacgtaatc agaatatcca gcgctgtttc cgtggttaat gatttcatgt tgtgaatttc
     1141 cggattacct gtcgaaagtg ggtaaacccg gcagatatcc ggcactggca tccagatgaa
     1201 tgggactgac accataacgc cggatgagtg tgacgaccag acgacggaac gtttttgaca
     1261 gccccaggcg cttaagccgc agaaccgggt atgaccacgc atcggtacgt aacagataac
     1321 cggtaccggt aaaatgaatc cattctgatt caccgaagtc actggtctgg tgtgacagcg
     1381 agtacagcca ggcgttgtcc gtttccgtga tatgtgcggt actgcagcgt atgccggtaa
     1441 gggcaacaaa tggtggcgtg cagtctgctg aggtccggtc agattcatca acaacacgaa
     1501 gggagtagcc gttatcgcag accatgttaa tcagttcagc gagattaaca ccattgcgta
     1561 ttaccgtgaa ggagatcggt gagtaacatc gatggagatc ggttcgtgtc actttcacag
     1621 aaccgttttt aaattacctt cactgatctc cttcggtcaa cggagattgt attttccgca
     1681 ttgattctcc tttaagttcg atcttgatac tgccatgaac cagtcgatct aggatggcat
     1741 ccgcatgtgt ggagtctccg atcattttgt accagttttc caccggcaac tggctcacta
     1801 cgatgattga gcctcgttgg tacatcagat ccactatttc cagcaggtcg ctacgctgtt
     1861 ctgatgagag aggttccagc ccccagtcat ccagaagcag cagatcgcta ttattcagcc
     1921 tggtcaaaag tttgctgtat cttccatcag catgcccctg atagcactgt tccatcagcg
     1981 ctttaaggcg ataatagtag accttgtatc cctgtcggca ggcattatga ccaagtgcac
     2041 atgccaggaa cgttttaccg ctgccggtgg ccccggtcag taaaatattt tgtttcaggg
     2101 ttaaccagtt tccctgactg agtgaacgga tgagggccct gtccagccct ctattgttac
     2161 gataatccag ctttgataac tcagcattaa gtctgaaccg tgcatgtttg atcagacgct
     2221 ctgccttcct gttttcacgg caggttagtt cttctgctgt cagtaatgac aggcgttctt
     2281 cgaagcccag ctcctggtat gtgcccggtt gagcaagttg ctttttaagc gcatcacgga
     2341 agccggtgag tttaagtgcg gtcagttgtt cgtaaagatg attcatcatt ggatcccgta
     2401 tcagtggtaa tactcactgc cgcgtacgtt ttcgtgttcc atcgtggata acagatctgg
     2461 ttttggatcc tgaacaggtt gtttatccag acctttttcc aggatcgatt taatacctga
     2521 cagacgccat acctttgttt tcagagctct tgcacatgct gcattaagtc tggctttact
     2581 gtatttttta tgaaggttca ggagtccaag gcagaagcga tagctttgtt ccggatgtgg
     2641 acgagagttc agtatatgaa gcacataact atgagtttca ctgcctatgt gccccgccca
     2701 ttccagaaga cgctctggcg tccaggtggc atgctgtcga tgagcttcag gcatgtgctc
     2761 gttgcgggta ctgtagccat aagtacgctt gcgcgggtgc acagcaacct cctgcccctg
     2821 attgaagagt cttaccagtt ctccggagat ccatgcttcc agttgctggc ctaacagcga
     2881 acatggaacc gagtagtaat gtttgtcgat ttccacgtgg taatcggcat gaactctgac
     2941 tttcttcacc agggtgtaac tgtaactggc ttcaggaaga ggcttcagtg cgggtttatc
     3001 aagctggatg aagagttctg cacgtgaata acccaacttc tgcattattt tgttattcag
     3061 tctttccagc aactcccgaa tgcgctgatt aagcgatgca aggctgtaga agatctcatg
     3121 cctgattcgg gccatgatcc agcgttcaac aacctgaacg ccaacttcag ctttggcttt
     3181 atctttcggt ttacgtggcc gcgcaggcaa aactgcgaca ttataatgct caagcatctg
     3241 ctggtaggta gggttaacgt caggatcata cttacatgcc ctggatgtgg cgcttttcag
     3301 attgtccgga acaacaagtt caggaacgcc acccaaccac tggaagcagc gaacatgact
     3361 catcacccag tcttcaagct gctgagacca ggtggcctct gcccatgtgt aacttgatgc
     3421 cccgagaaca gctacgatga cctgagcagt tcttatttct ccggtctcag ggtcggtaac
     3481 gccaacggta ggtccacagt aatcaacgaa aagtttttcg ccagctttat gtacctgacg
     3541 cattgatggt gaagtggttt tgagccattc acggtacatc cggcagtaat ggttatagct
     3601 gtaaaaaccg cctggattac gctcacagta ttcttcccag agtagctgca gcgtcacgca
     3661 tttattacgc agttcccggt gtactgtagc ccagtcaggc agagagtgct tcttcatctt
     3721 aacctgggtc tgaaggaacg catgttttag ttttgtatca tcccatcctg taggtaaggg
     3781 ccactgcttt atgccaagtt gagccgcccg attagcatat ctggatacaa cggaagggga
     3841 gattgcaaga ctacgaccaa tttgtcgatg gctgagtcca acaccgtatt taagcctaag
     3901 aatttcttta agttttctca tagaaattgg aactgttggc ataggtatcc tttaccggaa
     3961 tggcaaaaga tacagatcaa cacacctgtg aagttccaat aacattgatg gagatcactg
     4021 aataacaaaa tgagtcaaaa gtgatctcca tcgatgttat tcagcgatct gttcaaatgt
     4081 tattacccga tctcgatgga agttattgag tgat
//
LOCUS       DACBVI010000252         4096 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.255, whole genome shotgun sequence.
ACCESSION   DACBVI010000252 DACBVI010000000
VERSION     DACBVI010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.255"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            105..1625
                     /locus_tag="IU325_22160"
     CDS             105..1625
                     /locus_tag="IU325_22160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HAP5811688.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAVMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(1654..2277)
                     /locus_tag="IU325_22165"
     CDS             complement(1654..2277)
                     /locus_tag="IU325_22165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP5811689.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            2555..3319
                     /gene="nfeF"
                     /locus_tag="IU325_22170"
     CDS             2555..3319
                     /gene="nfeF"
                     /locus_tag="IU325_22170"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HAP5811690.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLHVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQSDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHEGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRCRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            complement(3373..3448)
                     /locus_tag="IU325_22175"
     tRNA            complement(3373..3448)
                     /locus_tag="IU325_22175"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3413..3415),aa:Ile,seq:cat)
     gene            3573..4079
                     /locus_tag="IU325_22180"
     CDS             3573..4079
                     /locus_tag="IU325_22180"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HAP5811691.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
BASE COUNT          980 a         1030 c         1123 g          963 t
ORIGIN      
        1 aattgcgatc taaatcaaat taatcagtta aagataaccg cagcggggcc gacataaact
       61 ctgacaagaa gttaacaacc atataacctg cacaggacgc gaacatgtct tctcatccgt
      121 atgtcaccca gcaaaatacc ccgctggcgg acgataccac tctgatgtcc actaccgatc
      181 tgcaaagcta tatcactcat gctaatgaca cttttgtgca ggtgagcggc tttaccttgc
      241 aagagttaca agggcagccg cacaacatgg tgcgtcaccc ggatatgcca aaagcggcgt
      301 ttgcagatat gtggttcacc ctgaaaaaag gggagccctg gagcggcatc gtgaaaaatc
      361 gccgcaaaaa tggcgaccat tattgggtgc gggccaatgc ggtaccgatg gtgcgcgagg
      421 gaaaaatcag tggctatatg tcgattcgta cccgggcgac ggatgaagag atcgcggcgg
      481 tggagccgct gtacaaagcg ttgaacgccg gacgtaccag taagcgtatt cataaaggcc
      541 tggtggtgcg taaaggctgg ctgggtaaac tgccttcatt accgcttcgc tggcgggcgc
      601 gtggcgtgat gaccctgatg tttatcttgc tggcggtcat gctttggttt gttgctgccc
      661 cggtggtgac gtatatcctc tgtgcgttag tggtattgtt ggcaagcgcc tgttttgaat
      721 ggcagattgt ccgcccgata gaaaatgtcg cccgtcaggc actgaaggtg gcgaccggag
      781 agcgtaatag tgttgagcat ctgaatcgca gcgatgagct ggggctgaca ttacgcgcgg
      841 tagggcagct tggcctgatg tgccgttggc taattaacga tgtctcaagc caggtgtcca
      901 gtgtcagaaa cggcagtgag acgctggcga aaggtaccga tgaactgaac gaacataccc
      961 agcagacagt tgataacgtt cagcaaacgg tggcgaccat gaaccaaatg gcggcgtcgg
     1021 tgaaacagaa ctctgccacg gcgtcggctg ccgataaact ttctatcact gccagtaatg
     1081 cggcagtgca gggcggggag gcgatgacca cggtgatcaa gacaatggac gatatcgccg
     1141 acagtaccca gcgcattggc accattactt cgctgattaa cgatattgcg tttcagacca
     1201 atattctggc cctgaatgcg gcggtggaag cggcgcgtgc cggcgaacaa ggcaaaggtt
     1261 ttgcagtggt ggcaggggaa gtgcgtcatt tagccagccg cagcgccaat gctgccaacg
     1321 atattcgcaa gctgattgat gccagtgctg ataaggtgca atccggttcg cagcaggtac
     1381 acgcagccgg acggacgatg gaagatattg ttgcacaggt gaaaaacgtc acccagttga
     1441 tagctcagat tagccattca acgctggaac aggccgatgg gctttccagc ctgacccgtg
     1501 cagtggatga gcttaacctc atcacccaga aaaatgccga gctggtggaa gagagtgcgc
     1561 aggtgtcggc gatggtgaaa caccgcgcca gccgactgga agacgcggtg acggtgctgc
     1621 attaatcgat atgccgcatc cggcaatcgg tgattaatcc agttgcgtaa tatcaaaagc
     1681 ggcgcggtcg atcaccgcaa tgatcttttt tatctgcgca tcagtgatat cgctctggtt
     1741 gacgcgtaaa tccagcaccg ctttaaaatt atccagtgcc cgcttcattt gcgggttctg
     1801 gcggagtgca gcgccaacgc aacgcgcttt gatgcgttct tcgatcatct ccacctgttc
     1861 gcggttttct tccagccact gcgcgccctg ttcggtcagc gcaatctgct tcttaccacc
     1921 ttcctcttcg cggatggtaa tcagcgactg ctcctggaga aaatccagcg tcgggtagat
     1981 gacgcccggg cttggggtgt aattcccctg ggttagattc tcaatcgctt taatcaactc
     2041 gtaaccgtgg ctgtcatcgc gcgagagaat atccagaatc accagacgca attcaccgtg
     2101 accaaagaaa cgctgccgac gaccgccgcc gcgaccgtgg cgaccaccgc agcattgacc
     2161 gtgttcatgc tggtgaccgt gtccgcagtg ctcatgttct gacttctcac ctttgcagca
     2221 gccttcatgg cgtggctggc cttcatgttt acaacaccct tcgtgatgat ggctcatatc
     2281 agtctccttt tgcttgatta gatatatctt atttatatct gatatgagcg ctcttgcaag
     2341 tataaaaatg atttttaaac gtggatgcac ctcacatcca ggccggataa tgcgttcacg
     2401 ccgcatccga aagccttgct acgacgctga atgtcttctc atacttcaga tatatcagtt
     2461 ctacaaatcg cttgcattta tcatgattaa caatcattat catttgcgaa attttattta
     2521 gatatatctg attaccatca tgaaggcgat aacaatgaat aactcccccc gctacccgca
     2581 gcgcgttcgc aatgatctgc gcttccgtga actgactgta ttgcacgttg agcgcatcag
     2641 cgccggtttt caacgcattg tcctcggcgg cgaggcgctg gacggcttta cgtcgcgcgg
     2701 ctttgacgat catagcaaac tcttctttcc tcaatctgat gctcactttg tgccgccaac
     2761 agtaacggaa gagggcatcg tctggccgga aggaccacgc ccaccgtcgc gtgactatac
     2821 accgctgtat gacgaactac gccatgaact ggcgattgat ttctttattc acgaaggtgg
     2881 cgtcgccagc ggctgggcga tgcaggcgca accgggcgat aaactcacgg tggcaggtcc
     2941 gcgcggttcg ctggtggtgc cagaggatta cgcgtatcag ctgtatgtct gcgatgaatc
     3001 cggaatgccc gcactgcgct gccgcctgga aacgttgagc aaacttgccg ttaaaccgca
     3061 agttagcgcg ctggttagcg tgcgggataa cgcctgtcag gattatctcg cgcaccttga
     3121 tggttttaat atcgaatggc tggcacatga tgagcaggca gtagatgcgc gtctggcgca
     3181 gatgcaaatc cctgccgatg attacttcat ctggataacc ggcgaaggca aagtcgttaa
     3241 gaatttaagc cgccgctttg aagcggaaca gtatgatcca cagcgggtac gtgcagcggc
     3301 ttactggcac gcaaagtaac tgatatataa aaagaaaaag gctgacgatt tctcgtcagc
     3361 ctttgctatg tctggtggcc cctgctggac ttgaaccagc gaccaagcga ttatgagtcg
     3421 cctgctctaa ccactgagct aaggggccat ggtagcggat tataaagtaa ctccgtgtcg
     3481 caatccagcc attaccgcgc gcctgctgtt tttataaaca atgtattttc aatccgttat
     3541 acttttcctg gtgatgttag aaaggagtaa atatggttga ggatattttg gctccagggt
     3601 tacgggtcgt gttttgcggt atcaaccctg ggctttcatc cgccgggact ggttttccct
     3661 ttgctcatcc ggcaaatcgc ttctggaagg tgatatatca ggccgggttt accgaccgtc
     3721 agttgaagcc gcaggaggca cagcatctgc tggattatcg ttgtggcgtc accaaactgg
     3781 tagaccgtcc aacggtgcaa gccaatgaag tttcaaagca ggagctacac gcaggcgggc
     3841 gtaagctgat tgaaaaaatt gaagattatc agccgcaggc gttggcgatt ctgggcaaac
     3901 aagcatatga acagggattc agccagcgcg gtgcacagtg ggggaaacaa acgctcacca
     3961 ttggttcgac gcagatttgg gtgctgccaa atcccagtgg tttaagtcgc gtttcactgg
     4021 agaaactggt tgaagcgtat cgcgagctgg accaggcgct ggtagtgcgt gggcgataaa
     4081 aaacgccacc gaaaag
//
LOCUS       DACBVI010000253         4088 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.256, whole genome shotgun sequence.
ACCESSION   DACBVI010000253 DACBVI010000000
VERSION     DACBVI010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.256"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(54..1220)
                     /locus_tag="IU325_22185"
     CDS             complement(54..1220)
                     /locus_tag="IU325_22185"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HAP5811692.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRYADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(1468..2874)
                     /gene="gndA"
                     /locus_tag="IU325_22190"
     CDS             complement(1468..2874)
                     /gene="gndA"
                     /locus_tag="IU325_22190"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP5811693.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSIKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGTDGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDKGEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(3261..3958)
                     /locus_tag="IU325_22195"
                     /pseudo
     CDS             complement(3261..3958)
                     /locus_tag="IU325_22195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1120 a          968 c          907 g         1093 t
ORIGIN      
        1 ttacaaaggg aacataagag aatcagggcg aaaatcgccc tgataacagg atgttagtca
       61 ctaccaaaga gatcgcgggt gtagactttg tctgccacat ccttaagctc ttctgccata
      121 cggttggaaa taatgacatc ggcttgttgt ttgaaggtgg cgagatcacg ttccaggcga
      181 gagttgaaga atgaatcttc tttcatcacc ggttcgtaga tgatcacttc aacgcctttc
      241 gccttgatac gtttcataat cccctgaatg gaagacgcac ggaagttatc tgacccgctc
      301 ttcataatca gacgataaat acccaccact tgcggcttgc gtgacaaaat ggcatcggca
      361 ataaagtctt tacgcgtgcg gttagcatcg acaattgccg agatcaggtt attcggcaca
      421 gactggtagt tcgccagtaa ctgcttggta tctttcggca gacaataacc accataacca
      481 aacgacggat tgttgtaatg gttgccaatg cgtgggtcga gacaaacgcc ttcgataatc
      541 tggcgagtat tcagacctaa actttctgca tagctatcca gttcattaaa gtatgctacg
      601 cgcatcgcca gataggtatt agcaaacagt tttatcgctt ctgcttcagt ggagtcggta
      661 aacagggtcg ggatattttg cttaatcgcg ccttcctgta acaacgcagc aaaacgttct
      721 gcgcgttctg aacgctcacc gatgacaata cgtgaaggat ggagattatc gtaaagagct
      781 ttaccttcac ggagaaattc cggagagaaa ataatatttt cagtacgata tttcttatgc
      841 attgctgcgg taaaaccaac gggaaccgtt gatttgatca ccataaccgc ataaggattt
      901 atctcaacta cgtctttaat tactgattct acactggatg tattgaaata attagtttta
      961 ggatcatagt cggttggagt ggcgatgatg acataatcag cataccggta ggcttcattt
     1021 ttatctaatg tggcattaaa gtgtatttta tctgattgca aaaactgctg aatttcctta
     1081 tcaacaatag gagatatccg atcattcagc atagcaacgc gtgacggtaa aatatctaat
     1141 gccacaacct catgattttg tgcgattaga agcccgtttg acaagcctac atagccggta
     1201 ccggaaatgg tgattttcat ttcatgctct cagaattaac ttaactgtga atcatgatgt
     1261 ttttagcatc ctgataagag ctaaaagttt taacgctacg ttgttagatg gtcaacgcaa
     1321 cgaaaaacaa ttcagatttt ctttataaga atagctgata tttattaagt taatattaag
     1381 cagtgaaatt tagtctataa gataattggc aaaaaaaagc ccggcgcaat atacgccagg
     1441 cctcaattta ttgttagtta aataagatta atccagccat tcagtatgga acacaccttc
     1501 tttatcaatg cgcttataag tatgcgcccc gaaatagtca cgctgtgcct ggatcaggtt
     1561 cgcaggcaga acagcggcac ggtagctatc gtaataggca accgcagcgg cgaaggtcgg
     1621 aaccgggata ccgttctgta ctgcataagc aacgacatca cgcagcgcct gctggtagtc
     1681 atcggcaatt tgcttgaagt acggagccag cagcaggtta gcgatctgcg gattttcggc
     1741 ataagcatcg gtgattttct gcaggaactg cgcacggatg atgcagccag cacggaaaat
     1801 tttcgcgatt tcgccgtagt tcagatccca gttgtactct tcagacgcag cacgcagctg
     1861 agagaagccc tgagcgtaag aaacgatttt gcccagatac agagcacgac gaactttttc
     1921 gatgaactca cctttgtcgc cagctggctg cgcttgcggg ccagagagaa ctttagatgc
     1981 ggcaacacgc tgctctttca gagaagagat ataacgtgca aacacagact cggtaatcag
     2041 cgacagcggt tcgccgagat ccagcgcgct ctggctggtc catttaccgg tacctttgtt
     2101 tgctgcttca tccagaatca catcaaccag gtagttaccg tcttcatctt ttttggtgaa
     2161 gatgtctttg gtgatgtcga tcaggtagct actcagttca ccgttattcc actcggtaaa
     2221 ggtctgcgcc agttcttcgt tagtgaggtt caggccacct ttaagcagag aataggcttc
     2281 agcaatcagc tgcatatcgc catattcaat accgttgtga accatcttca catagtgacc
     2341 tgcgccatcg gtaccaatat aggtaacgca cggttcgcca tcttcagcaa cggctgcgat
     2401 tttggtcagg atcggcgcaa ccagttcata ggcttctttc tgcccaccag gcatgatgga
     2461 aggccctttc agcgcgccct cttcaccacc ggaaacaccg gtaccgatga agttaaagcc
     2521 ttcggcagaa agctcacggt tacggcgaat ggtgtcctgg aagaatgtat taccaccatc
     2581 aatgatgatg tcacctttat cgaggtacgg cttgatggaa tcaatagcag catccgtgcc
     2641 agcacctgct ttcaccatta acaggatgcg acgaggcgtt tccagagatt caacaaactc
     2701 tttcaccgta tagtaaggaa ccagtttctt gcctggattt tcggcaatca cttcttccgt
     2761 cttttcacgg gaacggttga aaatagagac ggtataacca cggctttcga tgttgagcgc
     2821 aaggttgcgc cccatcactg ccataccgac tacgccgatc tgttgctttg acattgttta
     2881 ctcctgtcac gaaattaccg tcttagcggt tcggtttaaa cgtaatgtta acatacaaag
     2941 atacaactaa gaaaaaataa gagtgcaatc ttcagaatta ttatataatt acttccgaat
     3001 atattggtaa ataattttta agacctttag agggagtaat cgcggaatgc ttcttactaa
     3061 caaaatgcag aatccagata tatgtgactg caaaccaagt ttaactttta gccgataaag
     3121 ttggaattca ctttttatat acctcagccc ttttcgccta gccaacatat tatcaccaac
     3181 tctagctaat attaacgact cattaatgtt acacacatca aatccagatg ccactactcg
     3241 taaccggtaa tgactccaac ttattgatag tgttttatgt tcagataatg cccgatgact
     3301 ttgtcatgca gctccaccga ttttgagaac gacagcgact tccgtcccag ccgtgccagg
     3361 tgctgcctca gattcaggtt atgccgctca attcgctgcg tatatcgctt gctgattacg
     3421 tgcagctttc ccttcaggcg ggattcatac agcggccagc catccgtcat ccatatcacc
     3481 acgtcaaagg gtgacagcag gctcataaga cgccccagcg tcgccatagt gcgttcaccg
     3541 aatacgtgcg caacaaccgt cttccggagc ctgtcatacg cgtaaaacag ccagcgctgg
     3601 cgcgatttag ccccgacata gccccactgt tcgtccattt ccgcgcagac gatgacgtca
     3661 ctgcccggct gtatgcgcga ggttaccgac tgcggcctga gttttttaag tgacgtaaaa
     3721 tcgtgttgag gccaacgccc ataatgcggg ctgttgcccg gcatccaacg ccattcatgg
     3781 ccatatcaat gattttctgg tgcgtaccgg gttgagaagc ggtgtaagtg aactgcagtt
     3841 gccatgtttt acggcagtga gagcagagat agcgctgatg tccggcggtg cttttgccgt
     3901 tacgcaccac cccgtcagta gctgaacagg agggacagct gatagaaaca gaagccactg
     3961 gagcacctca aaaacaccat catacactaa atcagtaagt tggcagcatc acctactatt
     4021 gattacatac tctttactta tatcttcagt agaaaactgt tcggaaatta aattgttaat
     4081 tgacccca
//
LOCUS       DACBVI010000254         3951 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.257, whole genome shotgun sequence.
ACCESSION   DACBVI010000254 DACBVI010000000
VERSION     DACBVI010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.257"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            55..1713
                     /gene="dpiB"
                     /locus_tag="IU325_22200"
     CDS             55..1713
                     /gene="dpiB"
                     /locus_tag="IU325_22200"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HAP5811694.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIADKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKPGALEKGESYFITGKGSIGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMVIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1682..2362
                     /gene="dpiA"
                     /locus_tag="IU325_22205"
                     /gene_synonym="citB"
     CDS             1682..2362
                     /gene="dpiA"
                     /locus_tag="IU325_22205"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HAP5811695.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2403..3788)
                     /gene="dcuC"
                     /locus_tag="IU325_22210"
     CDS             complement(2403..3788)
                     /gene="dcuC"
                     /locus_tag="IU325_22210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAP5811696.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIADYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFIRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1082 a          943 c         1014 g          912 t
ORIGIN      
        1 caacatttta attacatctg tcaaagaggc tcgggacaac ccgcaaggaa aacaatgttg
       61 cagcttaacg agaataaaca gtttgcattt ttccaaagac tggcatttcc gctgcgtatc
      121 tttttgctga ttctggtgtt ctcaatattt gtcattgcag ccctggcgca atattttacg
      181 gccagttttg aggactattt aacgcttcat gtgcgcgaca tggcaatgaa tcaggcaaaa
      241 attattgctt ctaatgacag tatcatcagc gcagtgaaaa cgcgtgacta caagaggctg
      301 gcgaccatcg ctgacaaatt acaaagagat actgatttcg attatgtggt gattggcgat
      361 cggcactcga ttcgtcttta ccatcctaat ccggagaaaa ttggttatcc tatgcagttc
      421 accaaaccgg gcgcgctgga gaaaggggag agctacttca tcaccgggaa agggtcaatt
      481 ggcatggcga tgcgtgccaa aacgccaatc tttgatgacg atggaaaagt catcggcgtg
      541 gtgtcgattg gctacctggt gagtaaaatc gatagctggc gtgctgagtt tttattaccg
      601 atggcaggtg tgtttgtcgt gctgttaggg attctgatgt tgctatcgtg gttcctggcc
      661 gcgcatatcc gtcggcagat gatgggcatg gagccaaagc aaatcgcacg cgtggtccgt
      721 cagcaagagg cgctgtttag ttcggtttat gaagggctca ttgcggtgga tccgcatggt
      781 tacattaccg ccatcaatcg taacgcaaga aagatgctgg gtctgagttc ccccggacgg
      841 caatggttgg gtaaacccat tgctgaagtg gtcaggcccg ccgatttctt taccgaacag
      901 attgatgaaa aacgtcagga tgtggtggcg aactttaacg gtctgagcgt tattgccaac
      961 cgggaagcta ttcgttctgg tgatgatttg ctgggggcca ttatcagctt tcgtagtaaa
     1021 gacgaaatat ccaccctcaa tgcgcaactg acgcaaatta aacaatacgt cgagagcctg
     1081 cgcacattgc gacacgagca tctcaattgg atgtcgacgc tcaatggtct gttgcagatg
     1141 aaagagtatg atcgcgtgtt ggcgatggtg cagggggagt ctcaggccca gcaacagctt
     1201 attgatagcc tgcgcgaggc gtttgccgat cgccaggtgg cggggctgct ttttggtaaa
     1261 gtgcagcgcg cccgcgaact ggggctaaaa atggtcatcg tccccggaag tcagctttcg
     1321 caactgccgc caggactgga cagcaccgag tttgcagcca ttgttggcaa tttacttgat
     1381 aacgccttcg aagccagcct gcgtagcgat gaaggaaaca agatcgttga attattcctc
     1441 agcgatgaag gcgatgatgt ggtgattgaa gtcgccgatc agggctgcgg cgttccagag
     1501 tctctacgag acaaaatatt tgagcagggt gtcagtacgc gtgctgacga gcccggcgaa
     1561 catggcattg ggttgtactt gattgccagc tacgtaacgc gctgcggtgg tgttatcact
     1621 ctcgaagata atgatccctg cggtacctta ttttcaatct atattccgaa agtgaaacct
     1681 aatgacagct ccattaaccc tattgatcgt tgaggacgaa acgccgctgg cagagatgca
     1741 tgcggaatat attcgtcaca ttcccggatt cagtcagata ttactggcgg gaaacctggc
     1801 gcaggcccga atgatgatcg agcgttttaa gccggggcta atcttgctcg ataactatct
     1861 tcctgacggt agaggaatta atttactgca tgaactggtg caggcgcatt atcccggcga
     1921 cgtggtgttt accactgcag ccagtgatat ggaaacggtg tctgaagccg tacgttgtgg
     1981 tgtatttgat tatctcatta agccaattgc ctatgaacgg ctggggcaaa cgctaacccg
     2041 tttccgccag cgtaaacata tgctggaaag tattgatagc gccagccaga agcaaattga
     2101 tgagatgttt aatgcctatg ctcgcggtga acccaaagac gaactgccga ccggcattga
     2161 ccccttgacg ctaaacgcgg tgcgaaaact gtttaaagag cctggtgtgc aacatacggc
     2221 agaaacggtg gcgcaggcac tgactatcag ccgcaccact gccagacgtt atcttgaata
     2281 ttgcgccagc cgccatctga ttattgctga aattgttcac ggcaaagttg gcagaccgca
     2341 acgcatatac cacagtgggt gacatgataa agccggagga aacttgcctc cggcatgact
     2401 atttacttgc ctgtgaccgc ggctgccgtt cctggcacca tcagctctgt agcaacgata
     2461 acaatcacca gcccaacaag caccggcacc gaggtgcgtt ttacgacttc aaatggtgag
     2521 attttcgcca tcccggcaac agcaacgact acgccagaaa cgggtgacag ggtacgaccg
     2581 aggtttgacg cctgcagcat tgggatagtc aaatacgccg ggttaatgcc ggaagagtgc
     2641 gccagtttcg ggatcatctc aacaaacgca taaaacggcg cattgcctga accggtcgtg
     2701 actgccgcca gcattgtcag gatcactaat accagcatca ggatgatact cgccgaacca
     2761 aacgaggtgg cgatagaaat cagactttga ataaagccga tggtgctaag cccctgagcg
     2821 aataccccag cggcaaccag cagcatcacc acgttagcaa acgcatctgc catcccgcga
     2881 taagccactt ccagaccaga gaaaactttc tgggtattaa agctgcggat gaactccaga
     2941 atggaggcaa tcagcataca aatcaccaga atagtgatga tgtgtaattg cggaccccat
     3001 ttgccgtcaa aaatcagcac tccgatgatc ggcgtgaacg gcaaaatggc ataaaacgca
     3061 ggagcagtgg tggtgatttc actgacatct aacatttcat gagagatgtg ctctttttta
     3121 tccagataac gttgccagaa gaagtgggcg atcgccatgc cgataattgc agcaattgag
     3181 ataggcagcg tcgttttgaa ggcgaagtca atcagcgaca tttcggaagc ctgcgccgcc
     3241 agcaccacat cccctgaagt cggtgcgaga ataatcgccg ccggggaggc acaaatagca
     3301 gcagctgcac cacggctgat accaacgttt accatcaccg gaaacagggt tgccatcagc
     3361 aaaacaccca gaccggtcgc ggaagagacg gccagagaca tcagacaggc gacaaaatag
     3421 tcggcaatca tcagcaggta aggggagtta atatactgca atggttttga cgccagcttg
     3481 accaccatat cattcgcgcc gatatgggtc atgtaagcgg caaagccaca cagcatcata
     3541 atcatcatgc cgaggtcgcc gccgcggctc attagcaata ttttaacgta ttcaacgata
     3601 tccgtggcgc tgtagcctgt tgaagcctgg ctggacggta acactttgtg ccccataatg
     3661 gcactgataa tcagcaataa caggccaccg acaaataaca cgccagtggc ggaataccct
     3721 ttaatgatgt agcgagctac acccacaata accacaaccc caataaggag ttcaatgaat
     3781 gtcagcataa tttttcctgt ctccaggccc caaagtaaat aataaaaaat tcctaaaact
     3841 taaggaaaaa atatgcccaa taaattggcg atgaatgctg attaaaatca agaaaaactg
     3901 ccattaagac attgaagttg ctgtttttat acatagataa caaaacgcat a
//
LOCUS       DACBVI010000255         3826 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.258, whole genome shotgun sequence.
ACCESSION   DACBVI010000255 DACBVI010000000
VERSION     DACBVI010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.258"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            155..1339
                     /locus_tag="IU325_22215"
     CDS             155..1339
                     /locus_tag="IU325_22215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HAP5811697.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
     gene            1450..2373
                     /locus_tag="IU325_22220"
     CDS             1450..2373
                     /locus_tag="IU325_22220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HAP5811698.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
     gene            complement(2377..3195)
                     /gene="nlpA"
                     /locus_tag="IU325_22225"
     CDS             complement(2377..3195)
                     /gene="nlpA"
                     /locus_tag="IU325_22225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HAP5811699.1"
                     /translation="MKLTTHHLRAGAALLLAGILLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITENPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            3405..3698
                     /locus_tag="IU325_22230"
     CDS             3405..3698
                     /locus_tag="IU325_22230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811700.1"
                     /translation="MKPTMLLMITVFLTFPAISQAESPFSSLQSAKKKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDESNKQHIREAIVAMERNNQNNYWEALGKVECPDM"
BASE COUNT          923 a          912 c          860 g         1131 t
ORIGIN      
        1 ttttgctaat aatctcaatt gctggcccta taatattgcg ctagcattgc tttctggttg
       61 tatcagcgat actcaaaaat tcttaataca atactcattc gactggtact tatttgtaac
      121 tcagttatat tttgtcgccc ggtgattcag aagaatgcaa aaaacggcta ccactccatc
      181 aaaaatactt gatctcactg ccgcggcatt tttacttgtc gcctttctga cgggtattgc
      241 gggcgctctc cagactccta ccctaagtat attcctcgca gatgaactga aagcccgtcc
      301 tataatggta ggttttttct tcaccggtag cgctattatg ggaattcttg tcagtcaatt
      361 tctggcaaga cactccgata aacaaggcga ccgtaaatta ctgatcctgc tatgttgctt
      421 atttggagtg ctggcctgca cgctttttgc gtggaatcgc aactacttca ttctcctctc
      481 tacgggcgta cttctgagta gttttgcttc caccgcaaac ccgcaaatgt tcgccctcgc
      541 ccgtgaacac gccgacagaa caggccgtga gacggtaatg ttcagtacat ttttacgtgc
      601 tcagatctcg cttgcctggg ttatcgggcc accgctcgct tatgagctgg caatgggatt
      661 tagttttaaa gtgatgtatc tcaccgcagc catcgcattt gttgtttgcg ggctgatagt
      721 ctggttgttt ttgccatcaa tacaaagaaa tattcctgtc gttacccaac ccgtagaaat
      781 tttaccctcc acccatagga agcgggatac acggctactt ttcgtggttt gttcaatgat
      841 gtgggcggcg aataatctct acatgataaa tatgccgcta tttattattg atgaactgca
      901 tctaaccgat aaactggctg gagaaatgat tggtattgct gccggtctgg aaattccgat
      961 gatgttaatc gcaggctatt acatgaaacg tattggcaag cggctattaa tgctcattgc
     1021 tatcgtgagt ggaatgtgtt tttacgccag cgtactcatg gcgacgactc cggcagttga
     1081 gctggaattg caaattctaa atgccatctt ccttggtatt ctctgtggta tcggcatgct
     1141 ttattttcag gacctgatgc ctgaaaaaat aggctctgcg acaacgttat atgcaaatac
     1201 ttcacgcgtc ggctggatta tcgccggctc tgttgacgga attatggttg aaatctggag
     1261 ctaccacgcg ttgttctggc tggcgatagg gatgttgggt attgcgatga tttgcctgct
     1321 gtttattaaa gatatttagc tcacctgtct gggctaagaa aaatcggagt ggcgcaaaca
     1381 gagccatcct cgccattttt gctatcatgc ctgcatacat aaacgacaaa acagtatgca
     1441 gagggaaaaa tgggttccac cagaaagggg atgctgaacg ttctgattgc cgccgtgttg
     1501 tggggaagtt caggggtctg cgcgcaatac atcatggagc aaagccagat gtcgtcgcag
     1561 tttttgacta tgacgcgttt gatattcgcc ggtttgattc tgctgacgct gtcatttgtt
     1621 catggcgata aaatcttttc tattattaac aatcacaaag atgccattag tctgctgatt
     1681 ttttccgtgg ttggcgcgct aaccgtacag ctcacttttt tgctaaccat tgaaaaatcg
     1741 aatgctgcca cggcaacggt gctgcaattc ctctcaccga cgattatcgt cgcctggttc
     1801 tcactggtgc gtaaatctcg cccgggcatt ctggttttct gcgctatttt gacatcgctg
     1861 atcgggactt ttttattggt gacacacggt aatccgacgt cattatcgat ctctcctgcc
     1921 gcgttattct ggggcattgc ctcggcattt gctgcggcat tctataccac ctatccctca
     1981 acgctaattg cccgctatgg cacgttgcct gtcgtcggct ggagtatgct gatcggcggt
     2041 ctaattctgt tgccttttta tgccagacaa gggacaaact ttgtcgttaa cggcagtttg
     2101 attctggcgt ttttttattt ggtggtcatt ggtacgtccc tgacatttag tctgtacctg
     2161 aaaggagcac aattaattgg cgggccaaaa gccagcattt tgagctgtgc agagccatta
     2221 agtagcgcgc tgctctcttt gctgttgctg gggatcacat tcacattacc ggactggctg
     2281 ggcacactgc tgattctgtc atcggtgatt ttgatttcaa tggattcccg tcgccgcgcc
     2341 agaaaaataa atcgtccggc gcgggatgag tgagaattac cagccaggca ccgcgccacc
     2401 gttaaagatg gtttctgccg ctttagcgac ttcggatgat tgataagatt gcagaaattc
     2461 cttcacgttt tctgcattct tattatcttc ccgtgctacc agaatattca catacggcga
     2521 attcttatct tcaataaata cgctgtcgtg caccggagaa agcccggtct gctgaatgta
     2581 agtggtgctg ataatcgcca catcaacttt gggatcatcc agtacgcgcg ggagctgcgc
     2641 cccctcgagt tccataatct gcaaatgacg aggattctcg gttatatcca gcgcggtagg
     2701 tagtaaccct tttccctctt tcagagtaat tagcttctct ttttgcaaca gtaaaagcgc
     2761 gcgaccaagg ttggtcgggt cgttggggat cgccactgtc gcgccttctt ttatttgcgc
     2821 cacggttttg atttttttgg aataacccgc catggggaag acaaacgtat ttcccaccgc
     2881 taccagttta taaccatgcg cctgattatc ttgttcaaga aagggtcgat gctggaagac
     2941 gttcgcatca agctcgccat gatttgtcgc atcgttcggt agcaacgaac cgctaaatcc
     3001 taccaattca acatcgaggc catatttctc ttttgccacc tttttagcga cttccgcgac
     3061 atcttgttct gcgccattaa taacgcccac tttaatgtgc ttttcatcgc tgctgctctg
     3121 gtcgcaacct gccagcagaa ttcctgccag taataatgcg gcccccgccc gtagatgatg
     3181 tgttgtcagt ttcacttttt tatccttttt ttaatcgtta actgactata atggcaagat
     3241 cactccgatt taaaaaacga aaagctatcg aatagaagaa aaaggtatat aaaactggca
     3301 cttcttactg accacgcaaa cacctttcgt acagcatgat gagagcggca aaagcaaact
     3361 acacttatgc ttcattcgat gcacatttgc agaaggttgc cgctatgaag ccaacgatgc
     3421 tactcatgat taccgtattc ctgactttcc cagccatttc tcaggccgaa tcgccattca
     3481 gttctctaca gtcggcaaaa aaaaagacca cggtattgca agatttacgt aaaatttgca
     3541 cgccacaggc gtcattatca gacgaagcgt gggaaaaatt aatgttgtct gatgagagta
     3601 acaaacaaca tatccgcgaa gctattgtgg cgatggagcg taataatcag aataactact
     3661 gggaagcgct gggtaaggtg gaatgcccgg atatgtgaat ttcgcacacc tccgcgctca
     3721 ggccagcagc cgctgactta agctcgcaca tcagtaaatc acctgctttt tcaaattcca
     3781 atcaaaattg caccagttca tcaggattgt ggataaattt ccctct
//
LOCUS       DACBVI010000256         3750 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.259, whole genome shotgun sequence.
ACCESSION   DACBVI010000256 DACBVI010000000
VERSION     DACBVI010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.259"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            136..1374
                     /locus_tag="IU325_22235"
     CDS             136..1374
                     /locus_tag="IU325_22235"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HAP5811701.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISHWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            complement(1439..1510)
                     /gene="ypdK"
                     /locus_tag="IU325_22240"
     CDS             complement(1439..1510)
                     /gene="ypdK"
                     /locus_tag="IU325_22240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HAP5811702.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(1866..2786)
                     /gene="lpxP"
                     /locus_tag="IU325_22245"
     CDS             complement(1866..2786)
                     /gene="lpxP"
                     /locus_tag="IU325_22245"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HAP5811703.1"
                     /translation="MFPKCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPADENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            3139..3381
                     /locus_tag="IU325_22250"
     CDS             3139..3381
                     /locus_tag="IU325_22250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2545 family protein"
                     /protein_id="HAP5811704.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            complement(3458..3733)
                     /gene="ypdI"
                     /locus_tag="IU325_22255"
     CDS             complement(3458..3733)
                     /gene="ypdI"
                     /locus_tag="IU325_22255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HAP5811705.1"
                     /translation="MKVNLMLFSLFLLVSIMACNVFAFSISGGGSEASYKETEKTSAM
                     TTTHSTKFQPSQAILLKMREDAPPLNLTEEIPPPFPTKANYLIHPVR"
BASE COUNT          954 a          889 c          945 g          962 t
ORIGIN      
        1 cctcatttaa agccattgat aaaaatgttc taaaacgctg gacagacaga aattaatcag
       61 gctattttca aaacagccac catcgtggca gcgtcgctcg gacggtccgg gcgctaacgt
      121 taatctgagg aaattatggc tgacactcgc cctgaacgtc gctttacgcg cattgatcgt
      181 ctcccgccct acgtttttaa tattaccgct gaactgaaaa tggctgcgcg tcggcgcggc
      241 gaagatatta tcgatttcag tatgggtaac ccggacggtg cgactccgcc gcacatcgtc
      301 gaaaaattat gtacggtggc ccagcgcccg gacacgcatg gttactccac ttcacgcggc
      361 attccgcggc tacgtcgcgc catttcacac tggtatcagg atcgctacga cgtcgagatc
      421 gacccggaat cagaagccat cgtcaccatt ggttcgaaag agggactggc gcatctgatg
      481 ctggcgacgc tggatcatgg tgacactgta ctggtgccta atccaagcta cccgattcat
      541 atttacggcg cggtgattgc cggggcgcag gtacgctcag tgccgctggt ggaaggtgtc
      601 gatttcttca acgaactgga acgcgccatt cgcgaaagtt atccgaaacc gaagatgatg
      661 atcctcggct tcccgtcgaa cccaaccgcg caatgcgtgg aactggaatt ctttgagaag
      721 gttgtggcgc tggcgaaacg ctacgatgtg ctggtggtcc atgacctggc ctatgccgat
      781 atcgtctacg atggctggaa agcgccgtca atcatgcagg tgccgggtgc acgcgatgtg
      841 gcggtcgagt tctttacgct gtcgaaaagc tacaacatgg cgggctggcg tatcggcttt
      901 atggttggca acaaaacgct ggtcagcgcg ctggcacgta ttaaaagcta tcacgattac
      961 ggcaccttta cgccgttgca ggtggcagcg attgcggcgc tggagggcga tcaacagtgc
     1021 gtgcgcgata ttgccgaaca gtacaaacgc cgccgtgatg tactggttaa agggctgcat
     1081 gaagcgggct ggatggtcga aatgccgaag gcttcgatgt atgtctgggc gaaaatcccg
     1141 gaaccgtatg ctgccatggg ctcgctggaa tttgccaaga agctgctgaa cgaagcgaag
     1201 gtctgtgtct ctccggggat tggctttggc gactacggcg acacgcatgt tcgctttgca
     1261 ctgattgaaa accgcgaccg tattcgtcag gcgattcgtg gaattaaagc gatgttccgt
     1321 gccgacggtt tactacccgc cagcagcaaa catattcacg aaaacgcgga ataaacaccg
     1381 tcgtcagaaa tgcaaacagg agcctgatgg ctcctgtttt tcgtttcgca gtttactttt
     1441 agatcatcag ggcaaaagta ccggcccaaa cgatgaccat aaaagaaatg cccataaaga
     1501 aatatttcac tttacatctc tccgcgtaat tactgttacg cctgaatgac ttcattacac
     1561 ctgatacagt gagttgacac cgggcaaaac gaccttaaaa gtcctgtatt ttaccgtggg
     1621 catcacccca gaattctgag ctaaactgct ccagacggcg ctaatgtacg cctaaaactg
     1681 aaacgagaca agaggcattt tcttatttac ttttagtaac ttacaggtgt ctcatatcgg
     1741 cgacagttta atttctgacg gtaataatta gagattcagc gaaggaaaaa accttaagaa
     1801 aaaagccata aaaaccatga ggttattatg gctgatttga ggagagaaag agtaagagca
     1861 gtttgttaaa tgtatagcga cgattctccc accgggcgcg ttttaaagcg acggtggatc
     1921 cagaggtact gctccggtgc gcgcatgatc tctttttcga taatcttgtt catataggca
     1981 gcggcttgat tttcatctgc cgggtagcct tccatctctg gggtgatgta caaacgatat
     2041 ccgctgtaat ccgcttttct taccatcgtt acggttaaca tggctgcgcc agagagacgt
     2101 gaaaggacat acgtaccatt ggttgtggcg acattttcca ccgcaaagaa cggcgcgaag
     2161 gagctgcctt tacgaccata atcctgatcg ggagcaaacc ataccgcttc acctttcttc
     2221 agtgcaccga caatgccgcg cagattattt ctgccgatca tcgctttgtt agagcgcatg
     2281 cgcccacggg tctgcaccca ttccatcagc tgattattat gtggacgata ggtagccatc
     2341 attggttggc acagtcccat cacgcggccg cccagttcca gcgacataaa atggacgccg
     2401 acaaccatta cgccgcgatt ttgcatttgt gcgcgtttaa ggttatccaa cccttcaaca
     2461 tcaaaccatt tacgtacgcg actgtcgggc cagaaccacg ccatgccggt ttctaccagc
     2521 gccatgccga gtgaacgaaa gttttcggca atcatcttct cgcgttcttc cgcagaatgc
     2581 tgtgggaaac aaagttccag gtttttacgg gcgatagatt cacgccgttt caggaatggt
     2641 cgcgccattg cgccaatacg cgtgccgaga aagcagagaa caggataagg aagctgtacc
     2701 caaagccaga gtacaccaag cccaaaccac gtgagccagt agcgaggatg cagaaactcg
     2761 cgggaaaatt tgcattttgg aaacatagtt accttctgta gatacgaatt gttgtatcgc
     2821 agaagattcc atgtcgacag cggtacactc aattgattag gccatcatgt acgacaatgg
     2881 ttcctgtaaa gagtgtaaag actagtcagg acataaccac gaggagcatg aatgcggtat
     2941 ctgtacttta tcactcgctg actgctttca gctactgatt attaatcgtg caaagtaaat
     3001 atttttccgt acgcaacagt cttgcattta acatgctgtt ttcattgatg cttagcaaaa
     3061 attgccagag gttaaggatt gtcttactat taaagtgact gaagttacag gttttttagc
     3121 gcggcaggag gttgagctat gatttattta tggatgtttc tcgccctgtg tattgtctgc
     3181 gtaagcggat acatcggtca ggtactgaac gtggtatcgg cggtctcttc tttcttcggc
     3241 atggtgatcc tcgctgcact catttattac ttcaccatgt ggttaactgg cggtaatgaa
     3301 ctggtgacgg ggatatttat gtttcttgcc ccggcttgtg gcttgatgat tcgctttatg
     3361 gtgggatatg gcaggcggta gtgggtcagg atggctcatt tgggcaggtg ttcacatcag
     3421 catcagacat gattgttcac ctgccgtgtg acagacgcta tcgcacagga tggataagat
     3481 aattcgcctt tgtcggaaaa gggggcggaa tttcttctgt gaggtttaat ggtggcgcat
     3541 cttctctcat ttttaacaaa atcgcctgtg atggctgaaa ttttgtagag tgagttgtcg
     3601 tcatcgctga cgttttttcc gtctctttat agctcgcctc actaccacca ccagaaatag
     3661 aaaatgcaaa aacattgcat gccataatag agaccaataa aaataagcta aaaagcatta
     3721 agtttacttt catagaaagt tatcctgaaa
//
LOCUS       DACBVI010000257         3706 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.260, whole genome shotgun sequence.
ACCESSION   DACBVI010000257 DACBVI010000000
VERSION     DACBVI010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.260"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(115..1764)
                     /locus_tag="IU325_22260"
     CDS             complement(115..1764)
                     /locus_tag="IU325_22260"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP5811706.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSQAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVNYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            2289..3638
                     /gene="lysC"
                     /locus_tag="IU325_22265"
     CDS             2289..3638
                     /gene="lysC"
                     /locus_tag="IU325_22265"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HAP5811707.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLGAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
BASE COUNT          847 a          935 c          983 g          941 t
ORIGIN      
        1 aaaatttatt ttaggattaa cctcacagca tgatttccgt taaatcacag acaaggggat
       61 ttatctgata aaaaatgccg acataacgtc ggcattttta ttaaggttat ttaattaacc
      121 gcgccacgct ttatagcggt taatcagacc attggtcgag ctatcgtggc tgctgatttc
      181 tttatcatct ttcagctctg gcagaatacg gttcgccagt tgtttaccca gctccacgcc
      241 ccactggtcg aaggtgaaga tgttcagaat cacgccctga gtaaagattt tgtgctcata
      301 cagcgcaatc aacgcaccca ggctgaacgg agtgatttcg cgcagcagga tggagttggt
      361 cgggcggtta ccttcgaaca ctttgaacgg caccacgtag tcaagcgttg ccggatcttt
      421 accctgatca cgatattcct gctcaaccac ttcgcgggat ttaccaaacg ccagcgcttc
      481 ggtctgagca aagaagttag acagcagttt ctggtgatga tcagagagcg ggttatgggt
      541 gatagccggg gcgatgaaat cgcacggtac cattttggtt ccctggtgga tcagctggta
      601 gaacgcgtgc tgaccgttag tgcctggttc accccagata atcgggccag tctggtaatt
      661 cacgacgtta ccgttacggt caacgtactt accgttggac tccatattgc cctgctggaa
      721 gtacgccgcg aaacggtgca tatactggtc atacggcaga atcgcttcag tttccgcgcc
      781 aaagaaattg ttgtaccaga tgccaatcag cgccagtagt acaggcaggt ttttctcggc
      841 aggcgtggtg gagaaatgct tgtccatcgc gtgtgcgcca gaaagcagtt caacgaagtt
      901 atcaaagccg atggagagaa caatcgacag gccaatcgct gaccataaag agtaacgacc
      961 accgacccag tcccagaact cgaacatgtt ggcggtatca ataccaaact cgccaacggc
     1021 tttggcgttg gttgagagcg ccgcaaagtg tttcgcaacg tgtttttcat caccagccgc
     1081 tttcaggaac cagtcacgcg cactatgggc gttggtcatg gtttcctgag tggtgaaggt
     1141 tttagatgct accaggaaca gcgtggtttc cgggtttact tttttcagca cttccgcgat
     1201 gtgagtccca tcgacgttag aaacaaagtg catgttcagg tggtttttgt acggacgcag
     1261 tgcttcggtc accatgtatg ggccgaggtc agaaccgccg atcccgatgt tcactacgtc
     1321 agtgattgct ttgccggtat aacccttcca ctcaccggaa ataatcgctt gcgagaaggt
     1381 tttcatcttc tccagcaccg cgttgacttc cggcataacg tctttgccat caaccagaat
     1441 cggggtattg ctacggttac gcagcgctac gtgcagcacg gcgcggtttt cggtgcggtt
     1501 gatcttctca ccagagaaca tcgacttaat cgcgcccgcc agatcgcact ctttcgccag
     1561 atcttgtaat ttcgccagcg tctcttcagt gatgcggttt ttggagtaat ccaccagcat
     1621 ctgatcgtcg aaggttgcgg agaacttaga aaaacgatcg ccgtctttag caaaaagatc
     1681 ggcgatcgta acgtctttca tttcatcgaa gtgtttctgt agtgcctgcc aggcagcggt
     1741 ctgcgttgga ttgatgtttt tcattagcaa tactcttctg attttgagaa ttgtgacttt
     1801 ggaagattgt agcgccagtc acagaaaaat gtgatggttt tagtgccgtt agcgtaatgt
     1861 tgagtgtaaa cccttagcgc agtgaagcat ttattagctg aactactgac cgccaggagt
     1921 ggatgaaaaa tccgcatgac cccatcgttg acaaccgccc cgctcaccct ttatttataa
     1981 atgtactacc tgcgctagcg caggccagaa gaggcgcgtt gcccaagtaa cggtgttgga
     2041 ggagccagtc ctgtgataac acctgagggg gtgcatcgcc gaggtgattg aacggctggc
     2101 cacgttcatc atcggctaca ggggctgaat cccctgggtt gtcaccagaa gcgttcgcag
     2161 tcgggcgttt cgcaagtggt ggagcacttc tgggtgaaaa tagtagcgaa gtatcgctct
     2221 gcgcccaccc gtcttccgct cttcccttgt gccaaggctg aaaatggatc ccctgacacg
     2281 aggtagttat gtctgaaatt gttgtctcca aatttggcgg taccagcgta gctgattttg
     2341 acgccatgaa ccgcagcgct gatattgtgc tttctgatgc caacgtgcgt ttagtagtcc
     2401 tctcggcttc tgctggtatc actaatctgc tggtcgcttt agcggaaggg ctggaacctg
     2461 gcgagcgatt cgaaaaactc ggcgctattc gcaacatcca gtttgccatt ctggaacgtc
     2521 tgcgttaccc gaacgttatc cgtgaagaga ttgaacgtct gctggagaac attactgttc
     2581 tggcagaagc ggcggcgctg gcaacgtctc cggcgctgac agatgagctg gtcagccacg
     2641 gcgagctgat gtcgaccctg ctgtttgtcg aaatcctgcg cgaacgcgat gttcaggcac
     2701 agtggtttga tgtacgtaaa gtgatgcgta ccaacgaccg atttggtcgt gcagagccag
     2761 atgtagccgc gctggcggaa ctggccgcgc tgcagctgct cccacgcctc aatgacggct
     2821 tagtgatcac ccagggattt atcggtagcg aaaataaagg tcgtacaacg acgcttggcc
     2881 gtggaggcag cgattatacg gcagccttgc tggcggaggc tttacacgca tctcgtgttg
     2941 atatctggac cgacgtcccg ggcatctaca ccaccgatcc acgcgtagtt tccgcagcaa
     3001 aacgcattga tgaaatcgcg tttgccgaag cggcagagat ggcaactttt ggtgcaaaag
     3061 tactgcatcc ggcaacgttg ctacccgcag tacgcagcga tatcccggtc tttgtcggct
     3121 ccagcaaaga cccacgcgca ggtggtacgc tggtgtgcaa taaaactgaa aatccgccgc
     3181 tgttccgcgc gctggcgctt cgtcgcaatc agactctgct cactttgcac agcctgaata
     3241 tgctgcattc tcgcggcttc ctcgcggaag ttttcggcat cctcgcgcgg cataatattt
     3301 cggtagactt aatcaccacg tcagaagtga gcgtggcatt aacccttgat accaccggtt
     3361 caacctccac tggcgatacg ttgctgacgc aatctctgct gatggagctt tccgcactgt
     3421 gccgggtgga agtagaagaa ggtctggcgc tggtcgcgtt gattggcaat gacctgtcaa
     3481 aagcctgcgg cgttggcaaa gaggtattcg gcgtactgga accgttcaac attcgcatga
     3541 tttgttacgg cgcatccagc cataacctgt gcttcctggt gcccggcgaa gatgccgagc
     3601 aggtggtgca aaaactgcat agtaatttgt ttgagtaaat actgtatggc ctggaagcta
     3661 tatttcgggc cgtattgatt ttcttgtcac tatgctcatc aataaa
//
LOCUS       DACBVI010000258         3576 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.261, whole genome shotgun sequence.
ACCESSION   DACBVI010000258 DACBVI010000000
VERSION     DACBVI010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.261"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            59..1276
                     /locus_tag="IU325_22270"
     CDS             59..1276
                     /locus_tag="IU325_22270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP5811708.1"
                     /translation="MATAWYKQVNPPQRKALFSAWLGYVFDGFDFMMIFYILHIIKAD
                     LGITDIQATLIGTVAFIARPIGGGFFGAMADKYGRKPMMMWAIFIYSVGTGLSGIATN
                     LYMLAVCRFIVGLGMSGEYACASTYAVESWPKNLQSKASAFLVSGFSVGNIIAAQIIP
                     QFAEVYGWRNSFFIGLLPVLLVLWIRKSAPESQEWIEDKYKDKSTFLSVFRKPHLSIS
                     MIVFLVCFCLFGANWPINGLLPSYLADNGVNTVVISTLMTIAGLGTLTGTIFFGFVGD
                     KIGVKKAFVVGLITSFIFLCPLFFISVKNSSLIGLCLFGLMFTNLGIAGLVPKFIYDY
                     FPTKLRGLGTGLIYNLGATGGMAAPVLATYISGYYGLGVSLFIVTVAFSALLILLVGF
                     DIPGKIYKLSVAK"
     gene            1288..2406
                     /locus_tag="IU325_22275"
     CDS             1288..2406
                     /locus_tag="IU325_22275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP5811709.1"
                     /translation="MINYGVVGVGYFGAELARFMNMHDNAKITCVYDPENGENIAREL
                     QCINMSSLDALVSSKLVDCVIVATPNYLHKEPVIKAAKNKKHVFCEKPIALSYEDCVD
                     MVKACKEAGVTFMAGHIMNFFNGVQYARKLIKEGVIGEILSCHTKRNGWENKQERLSW
                     KKMKEQSGGHLYHHIHELDCVQHLLGEIPETVTMIGGNLAHSGPGFGNEDDMLFMTLE
                     FPSGKLATLEWGSAFNWPEHYVIINGTKGSIKIDMQETAGSLRIGGQTKHFLVHETQE
                     EDDDRRKGNMTSEMDGAIAYGHPGKKTPLWLASLIRKETLFLHNILCGAKPEEDYIDL
                     LNGEAAMSAIATADAATLSRSQDRKVKISEIIKHTSVM"
     gene            2449..2574
                     /locus_tag="IU325_22280"
     CDS             2449..2574
                     /locus_tag="IU325_22280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811710.1"
                     /translation="MFKNFIFDNLIILAVPFMIKTSLKTNLIFFFLCVFVPHMAS"
     gene            complement(2627..2884)
                     /locus_tag="IU325_22285"
     CDS             complement(2627..2884)
                     /locus_tag="IU325_22285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811711.1"
                     /translation="MVSSSASNVVNCETKQRTQFECIYFSQYWAKGDFIAKRAPIGQW
                     EPYSEESLLGIIVTSVCRIKVAMLKPEPPRDPHIPLMGDFN"
     gene            2874..3110
                     /locus_tag="IU325_22290"
                     /pseudo
     CDS             2874..3110
                     /locus_tag="IU325_22290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939276.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            3198..3576
                     /locus_tag="IU325_22295"
                     /pseudo
     CDS             3198..3576
                     /locus_tag="IU325_22295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1053 a          646 c          781 g         1096 t
ORIGIN      
        1 gttgaggctc cctaatattt actttaaggg ctatattaga ataacacagg aaacaaatat
       61 ggcaacagca tggtataaac aagttaatcc accacaacgg aaagctcttt tttccgcatg
      121 gcttggatat gtatttgatg gctttgattt tatgatgata ttttacattc ttcatattat
      181 aaaagcagat cttggcatta cggatattca ggctacttta atagggacag tggccttcat
      241 agccagacct attggaggtg gtttttttgg tgccatggct gataaatatg gtcgtaagcc
      301 aatgatgatg tgggcaattt tcatttactc agtcggaaca ggccttagcg gtattgctac
      361 aaacttatat atgctcgcag tttgccgttt tattgttggc ttagggatgt ctggtgaata
      421 tgcatgtgct tcaacttatg cggtagaaag ttggcctaaa aatcttcaat ctaaagctag
      481 tgcttttttg gtaagtggtt tttctgttgg aaatattatt gcggcacaaa taatccctca
      541 gtttgctgaa gtatatggat ggagaaactc tttttttata ggcctgttac cagttttact
      601 agttctttgg atcagaaaaa gtgctccaga aagtcaggag tggattgaag ataaatataa
      661 ggataaatca acatttttgt ctgtcttcag aaaaccacat ctttcaatct ctatgatcgt
      721 tttcctcgtc tgtttttgtc tatttggtgc aaactggccg ataaacggac tacttccttc
      781 ctacctggca gataatggag ttaatacagt ggtcatttca actctgatga caatagcagg
      841 tttaggaaca ctgacaggta caatattttt tggttttgtt ggtgataaga ttggtgtaaa
      901 aaaagccttt gtagtcggtc taataacttc atttattttc ctttgtcctc ttttttttat
      961 ttctgtgaaa aactcttctc ttataggatt atgtctcttt ggattaatgt ttacaaattt
     1021 aggtattgca gggttggttc caaaatttat atatgattac tttccaacaa aattaagagg
     1081 attagggacc ggtcttattt ataacttagg ggcaactgga ggaatggccg cacctgtatt
     1141 agctacatac atttcaggat attatggctt aggtgtttca ttattcattg ttacggttgc
     1201 attctctgcc ttattaattt tgttagttgg ttttgatatt ccaggtaaaa tttataaact
     1261 atccgtggct aaatgattgg aggctttatg attaattatg gcgttgttgg tgttggatac
     1321 tttggcgctg aattagctcg ttttatgaat atgcatgata atgcaaaaat tacatgtgta
     1381 tacgatcctg aaaatggaga aaatattgcc cgtgaattgc agtgtatcaa tatgtcaagc
     1441 ttggatgctt tagtctcaag taaattagtc gattgcgtga tcgtagccac cccaaattat
     1501 ctgcataaag aaccagtaat taaagcagca aagaataaga agcatgtttt ttgtgaaaaa
     1561 ccaattgcat taagttatga agattgtgtg gatatggtca aagcgtgtaa agaagctggt
     1621 gtgaccttta tggccgggca tattatgaat tttttcaatg gggttcaata tgcacggaag
     1681 ttaattaaag aaggtgttat cggcgaaata ttatcatgtc atactaagag aaatggctgg
     1741 gaaaacaaac aagagagact ttcctggaaa aagatgaaag aacaatctgg tggacatcta
     1801 tatcatcata tacatgagtt agattgtgtt cagcatttac ttggagaaat accagagacg
     1861 gttactatga ttggtggaaa tttggcccat tctggtccag gatttggcaa tgaagatgat
     1921 atgttattta tgaccttgga attcccgtca ggaaaactag caaccttaga gtgggggagt
     1981 gcatttaact ggccggaaca ttatgtcatc atcaatggaa ctaaaggctc tattaaaatt
     2041 gatatgcaag aaacagcagg gtcacttagg attggcggtc agacaaagca ttttttggtc
     2101 catgaaacac aagaagaaga tgatgatcgt cggaaaggca atatgacctc agaaatggat
     2161 ggcgctatag catatggtca tccaggaaaa aaaacaccat tatggcttgc cagtttaata
     2221 agaaaggaga cgttattcct ccataatatc ctctgtggtg caaaacctga agaagattat
     2281 attgaccttc tcaatggtga ggcggccatg tcggcgattg ctactgctga tgctgccact
     2341 ctttcaagat cgcaggacag gaaagtgaaa atcagtgaga tcattaaaca tacatcagta
     2401 atgtaaaaac tatacgcctc ttgatttaat tcaggaggcc ttttatgtat gttcaagaat
     2461 tttatcttcg ataatctcat aattttagca gtgccattta tgataaaaac atcccttaaa
     2521 accaatctaa tctttttctt cctctgtgta tttgttcccc atatggcgtc ataatttacg
     2581 ctacgtaata cgggatagtt acgatacgca gcgatagcgc taagttttag ttaaaatccc
     2641 ccatcagcgg gatatgggga tctctgggag gttcaggttt tagcatcgcg actttaatac
     2701 gacagacgct ggtgacgatg atgccaagta gtgactcttc cgaataaggt tcccactgac
     2761 caattggcgc gcgcttcgca ataaaatctc ccttcgccca gtattgggaa aagtagatac
     2821 attcaaactg tgtacgctgt ttcgtctcac agttcacgac attgctggcc gatgagctga
     2881 ccattgggcc tatccgggct gtcccgatgg atattacgcc gaagtatgtg ggaattgcca
     2941 gcggattgat gaacgccggt tccgctgtcg ccgacattat ttcacctatc gcctttggca
     3001 ttattatcga caaaacaggc aactggagtt taccattcta cggttccgtc gcattgcttg
     3061 ttattggtat attcctgacg ttcttcatgc gtccagataa gtcattgtaa aagtcgaatc
     3121 agggctgaag tggcacactg aatttggcca cctgaacaga ggtgatatgc tcacctcaga
     3181 acaacacagg tgctccaatg aaaaaaagaa atttcagcgc agagtttaaa cgcgaatccg
     3241 ctcaactggt tgttgaccag aaatacacgg tggcagatgc cgcgaaagct atggatgttg
     3301 gcctttccac aatgacaaga tgggtcaaac aactgcgtga tgagcgtcag ggcaaaacac
     3361 caaaagcctc tccgataaca ccagaacaaa tcgaaatacg taagctgagg aaaaagctac
     3421 aacgcattga aatggagaat gaaatattaa aaaggctacc gcgctcttga tgtcagactc
     3481 cctgaacagt tctcgataat cgggaaactc agagcgcatt atcctgtggt cacactctgc
     3541 catgtgttcg gggttcatcg cagcagctac agatac
//
LOCUS       DACBVI010000259         3448 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.263, whole genome shotgun sequence.
ACCESSION   DACBVI010000259 DACBVI010000000
VERSION     DACBVI010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.263"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            100..255
                     /locus_tag="IU325_22300"
     CDS             100..255
                     /locus_tag="IU325_22300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811712.1"
                     /translation="MAKLMNNSSKTSTVQIKRIKPSIIYRLLLIGLGTPMVIYGLVRP
                     LTIETRD"
     gene            complement(482..2020)
                     /locus_tag="IU325_22305"
     CDS             complement(482..2020)
                     /locus_tag="IU325_22305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAP5811713.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMELVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMKRAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2221..3372)
                     /gene="yiaY"
                     /locus_tag="IU325_22310"
     CDS             complement(2221..3372)
                     /gene="yiaY"
                     /locus_tag="IU325_22310"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HAP5811714.1"
                     /translation="MAASTFFIPSVNVIGADSLNDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT          849 a          920 c          828 g          851 t
ORIGIN      
        1 ttatttatat aacgacatat tgttttttat atactttctg aaatgtgcag cctggctttt
       61 caacagtgcg aggcttatca tattgaaata atggaactat tggccaaact aatgaataac
      121 tccagtaaaa catccacagt acagattaag cgtattaaac cttcaattat ctaccgttta
      181 ttgctgattg gcctcggaac gccaatggtg atttatggtc tggttcgccc gctcaccatc
      241 gaaacgcgag attaaactcc tggcaaaaca cgccccagaa acgcacttct gttggcgtag
      301 tctgggttat tgggttattg cgcacgtagg tttaaaacgt aaattgtaac aacgtgcgct
      361 ttgtttatgc cggatgcggc gtgtacgcct tatccggcct acggtcaggt ccccgtaggc
      421 atgataaaac gcgtaagcgt cgcatcaggc aatgaatacc caatgcgacc agcttcttat
      481 atcagaacag ccccaacggt ttatccgaat agctcaccag caggcacttg gtttgctggt
      541 aatgctcaag catcatctta tgggtttcgc gtccgatgcc cgactgtttg tagccgccaa
      601 acgccgcatg tgccgggtaa gcgtgataac agttggtcca cacgcgccca gcctgaatgc
      661 cgcgccccat cttataggcc agattaccgt tgcggctcca gacacccgcg cccaggccat
      721 attgcgtgtc gttcgccagc tccagcgctt cttccatcgt tttgaaggtg gtcaccgcca
      781 gcaccggacc aaaaatttct tcctggaaca cgcgcatatt attctgacca aacagaatcg
      841 tcggttcgag gtagtagccg tctttcagtt cgccttccag cagcttgcgc cgcccgccgg
      901 tgagcacgtc tgcgccctct tttttaccga tatcaatgta gttgaggatg gtttccagtt
      961 gcccgtgaga aacctgcgcc cccatttgcg tcacgctgtc gagcgggtta ccgctgcgaa
     1021 tgctttcgac acggcggatg gcgcgtttca taaagcgttc gtagatagac tcctgcacta
     1081 aagcacggct tggacaggtg caaacttcgc cctggttaaa ggcaaacagt gcaaagcctt
     1141 ccagcgcttt atcgaaaaag gcatcttctt catccatcac atcagcaaag aagatatttg
     1201 gggatttgcc gcccagctcc agcgtcaccg gaataatgtt ttgcgtggcg tactgcataa
     1261 tttgttggcc cacttccgtt gagccggtaa acgccacttt ggcgatgcgt ttcgaggtcg
     1321 ccagatattc accaatttcc ccaccagcgc cgttaaccac gttcaccacg cccggcggca
     1381 gtaaatcacc gacgagttcc attagcagca gtacagaaag cggggtaaga cgtgcgggtt
     1441 tcagcaccac acagttgccc gccgccagcg cgggagccat tttccagctc gccatcagca
     1501 gcgggaagtt ccacggaata atctgcccca ccacgcctaa cggttcgtgg aagtgataag
     1561 ccacagtttc gctatccact tcactaatac cgccttcctg cgcccgaata cacgaggcaa
     1621 aatagcgaaa atggtcaatc gccagcggca catcagcagc actggtttcg cgaatgggtt
     1681 tgccgttatc ccaggtttca gcggtcgcta acagttcgag gttctgctcc atccgatcgg
     1741 caatcttaaa cagaatcgcc gcgcgatcct gcaccgaagt atgcgcccat ttatctttca
     1801 ctttgtgcgc agcatccagc gccagatcga tgtctcgttt gcccgaagac gccacttcgc
     1861 acagcagctg cccggtcacc ggcgtcaggt tctggtaata ctcaccatcg gcaggggcta
     1921 cccattcgcc gccaataaag ttgtcgtagc gggcttttaa cttgaggggg aaaccatact
     1981 cgccgggctt aatctgtgct gaagggggat tattggtcat gatcgtctcc ttgcggtgtg
     2041 aggtataaca agggtagacg tgactggcga aatcttcgcc agtcggtaac agctttacga
     2101 cggctatcac gaatttacgg gcaaggatta cagagataac taaaactctc tcaaaaatcg
     2161 caaattttga gatagttaaa aaaatggctg acaattagcc agccattggg ggaatgatga
     2221 ttacatcgca gcgcgataaa ttgccacaat ctcttcgtga gttgcctgaa tcgggttagt
     2281 aaacccacac gcatctttca gggcattggt ggccagtacc gcgaaatcct cttctttcac
     2341 gttcaggtca cgcaggcctg ccgggatatc cactttcttc gccagttcac ggatggcatt
     2401 gatgcacgct tctgcgcctt ccgcgtcatt tttgcctgtc acgttaacgc ccatggcagc
     2461 agcacagtca cgcagacgcg cagcagcgac tttgctgttg aacacctgaa cgtgtggcag
     2521 cagaacggcg ttacatacac cgtgcggcag attgtagaaa ccgcccagct ggtgcgccat
     2581 cgcatgaaca taacccagag aagcattatt gaacgccata ccggcgagga actgggcata
     2641 agccattgct tcacgcgctt tcgcattact gccatcttca acggctaacg gcaggttttc
     2701 ggcaatcatg gtcacggctt tcagtgcaca agcgtcagtg atcggcgtgg cggcaataga
     2761 aacatacgct tcgatagcgt gcgttaaggc atccataccc gttgcggcgg tcagtgactt
     2821 cggcatacca atcatcagag aggagtcatt gacagaaagc agcggagtta catgtttatc
     2881 cacaatcgcc attttgatgt gacgcgcttc gtcagtgatg atgcagaaac gggtcatttc
     2941 agaggccgta cccgccgtgg tgttgatggc gatcatcggc agctgcggtt ttgcagagcg
     3001 gtcaacgcct tcataatcgc gaatatcgcc accattggct gccaccagcg caataccttt
     3061 tgcgcagtcg tgtggagaac caccgcctaa ggaaatcacg ctatcgcaat tattttcttt
     3121 aagtaatttc aaacctgcag cgacgttttc cgtggtgggg ttaggttggg tgccatcata
     3181 aataacacta aaaatattgc gttcttccag tgctttttgc acatcacccg ccatacccaa
     3241 tttcgttaac atattgtcag tgacaattaa ggtacgggta aatccataat ctgccatcat
     3301 attcattgca tcattcaatg aatcagcacc aataacattc acggaaggaa taaaaaaggt
     3361 tgaagctgcc ataacacact cctgaaagtg taaaaaaggg cgggcagctt actgaggatt
     3421 ttcatttgaa aatatgatca gtattatt
//
LOCUS       DACBVI010000260         3435 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.264, whole genome shotgun sequence.
ACCESSION   DACBVI010000260 DACBVI010000000
VERSION     DACBVI010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.264"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(250..2907)
                     /locus_tag="IU325_22315"
     CDS             complement(250..2907)
                     /locus_tag="IU325_22315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HAP5811715.1"
                     /translation="MMINIKKKEVSILLAILASLLLTQSAYAADLFTVPETDKSKLWF
                     LDILFPDNLAESPLASTMTILNSAVLLVGGILAAYTLIAGTMSTAHDGEMLGKKWSSM
                     WLPVRTALGTAMILPAAGGFCAAQVMVLWMINQGVGLADTVWNTYASNPSDGAVITTS
                     ASYQELDRIAKTAFINNVCMLKASELWKKSAAADPFPHVTPAFEMTPEKGAYLYTYSY
                     GASNAGNDMNKNLLVSKAACGSITLTDPKAKASYDEQAKAQATIAAGGMYMAYMPQDI
                     KTNISDVIEAHNTAFIGLDNSMKTLAEQYVADNNIDIQTGINSATASYVSIIDTAVRT
                     VFSTGDQWDDFKENVQKDGWFMAGAWSMKLIRVQDAINGAAHNLPVGGQATMEYGDIF
                     DNSLNAIMAKVAQDMAKSTTASRYANGIDAQSRTEANTSGKSGAGPKTDDAGKIVSSL
                     QSEANKSISGAMAGFLSSSVINGRKQGKIVSFSTDTTAANLQAINPLLAVKGLGDTIS
                     AAGWTLLGSSAVVGAGLGAWTSWTASWVSGAFGAMGIVMPLVIPLWIAGDTLAVVIPM
                     LPYVMWFGVCVGWMILCLEAMVAAPLWVITHLHPDGDGVVGRGGAGYGLVLSLTMRPA
                     LMITGLIAAYTMLPILGGIVNETFSGAFGMMSANAGIGIIESLALIAVYIAMMFTVVK
                     KSLSLIHIIPDEIMKWLGVHGGQSMSGYAQSASKGVEGAMFTKTVLDQVSHTSNAMGN
                     QLRNAQINKDREQQRELQQQQQAKQGKAQAANKANDTGSAFRTHMSNAGPLDQQDEYQ
                     SLESANSAYNAAEAADAVGDTAGAAGYMNVAQKAANRAVSFGEHNRPLLPINLQAQAS
                     PIESFTAPDKGNGGGSSGGSSSPTESNDR"
BASE COUNT          907 a          842 c          697 g          989 t
ORIGIN      
        1 catataaaac aaacggagtg aatatcataa atgcagcgat atttttatat atttttgcct
       61 atgaaaaaaa cattttatag gagaataaaa ccttgcatga gaaatgccca tattcaataa
      121 aggctgtggt gaggttctat ttttcactat agtcatttaa attatgctgg taagcaaaca
      181 tataaagtga gttccattcc agataacgaa caattaaaaa gagggcgaaa gccctctttt
      241 tatatcacat tatctgtcat tactttcagt gggtgatgaa ctaccaccgg aagatccacc
      301 accattccct ttatctggag ccgtaaagct ttcaattggt gaagcttggg cttgaaggtt
      361 tataggcagc aacggtctgt tatgttcacc aaacgatacc gctctgttag cagccttttg
      421 ggctacattc atataacctg cagcgcctgc tgtatcacca acagcatctg ctgcctcagc
      481 cgcattataa gccgagttag cactttcaag agactgatat tcatcctgct gatccagtgg
      541 gccagcatta ctcatatgag ttctgaatgc agatcccgta tcattggctt tattagctgc
      601 ctgtgctttc ccctgtttag cctgttgctg ttgttgaagc tcacgttgct gttcacggtc
      661 tttgttgatt tgcgcattcc tgagctgatt acccatcgca tttgaggtgt gagaaacctg
      721 atccaacacg gttttggtaa acatagcacc ttcaacacct tttgatgccg actgtgcgta
      781 acccgacatg ctctgaccac catgaacacc aagccacttc ataatttcat cagggatgat
      841 gtgaatcagt gaaagtgatt tcttcactac cgtgaacatc atcgcaatat aaacagcaat
      901 gagagccagt gactcgatga tacctatccc ggcattggct gacatcatgc caaacgcgcc
      961 ggagaatgtt tcattgacga tcccaccaag gatagggagc atcgtatagg ctgctattaa
     1021 tcctgttatc attagcgctg gtcgcatggt taacgataaa accagaccat aaccagcgcc
     1081 accacgaccc actacgccat caccgtctgg atgtaaatgt gttattaccc ataatggcgc
     1141 tgcgaccatt gcttcaaggc acaggatcat ccagccgacg caaacaccaa accacatgac
     1201 gtaaggcaac ataggaataa cgacggctaa ggtatcacca gctatccata aagggataac
     1261 cagtggcatg acaatcccca tagcgccaaa tgcacccgat acccagcttg cagtccatga
     1321 agtccatgca ccaagacctg cgccaacaac cgcagaactt cctaataacg tccagccagc
     1381 ggcactgatc gtatccccaa gccctttaac ggctaataat gggtttatcg cctgtaaatt
     1441 ggcagcagtt gtgtcggttg aaaacgaaac aattttccct tgttttctgc cattgataac
     1501 gctggagctg agaaaacccg ccattgcacc agaaatgctc ttattggctt cactctgaag
     1561 agaggaaaca attttcccgg catcatccgt tttaggacct gcgccacttt taccactggt
     1621 attggcttct gttcggcttt gcgcatcaat accattcgca tatcttgaag ctgtcgttga
     1681 tttcgccata tcctgagcta cttttgccat aatcgcgttc agactgttat caaatatatc
     1741 tccgtattcc attgtcgctt gcccacctac aggaagatta tgagctgctc cattaatcgc
     1801 atcctgcaca cggatcagtt tcatactcca tgcgccagcc ataaaccatc catctttctg
     1861 gacattctct ttgaaatcat cccactggtc gccagtggag aaaactgttc gtactgcagt
     1921 atcaataatg gatacatatg aggccgttgc gctgtttatt ccggtctgaa tatcaatatt
     1981 attatcggcc acatactgtt cagccagcgt tttcatacta ttatcaagac cgatgaatgc
     2041 agtattgtgt gcctcaataa catctgaaat atttgtcttt atatcctgtg gcatataagc
     2101 catatacatg cccccagcag cgatggttgc ctgagcttta gcctgttcgt cataagaagc
     2161 tttggccttg gggtctgtaa gcgtgatgct tccacatgca gccttggata ccagtaaatt
     2221 tttattcata tcattaccgg cattgctggc tccataactg tatgtgtata aataggcacc
     2281 tttctctggc gtcatttcaa acgcaggcgt gacgtgcggg aagggatcgg ctgccgctga
     2341 tttcttccat aactcacttg ccttcaacat acagacgtta ttgataaacg cagttttcgc
     2401 tatacggtca agttcctgat aagacgctga tgttgtgata actgctccat ctgaggggtt
     2461 ggaggcataa gtattccaga cggtatccgc cagaccgact ccctgattaa tcatccacag
     2521 caccataacc tgagcggcac agaaaccacc tgctgccgga agtatcattg ccgtgcctaa
     2581 tgcagttctt acgggcaacc acatggaaga ccatttctta cccagcattt ctccgtcatg
     2641 tgccgttgac atggttccgg caatcagcgt gtaggcagct aaaataccac caaccagcaa
     2701 aacggcgctg ttaaggattg tcattgtgct ggctaaggga ctttccgcta aattgtccgg
     2761 gaataagata tccagaaacc acagctttga tttgtctgtc tccggaacgg taaataagtc
     2821 ggcggcataa gctgactgag ttaataacag actagctaat attgctaata gtatcgacac
     2881 ttcttttttc tttatattta tcatcattac attcctttgc ctcttacttc attgaattac
     2941 tgatgctgct ccagacatcg gttccattga cggttgctcc cgatcctgat gaagagccag
     3001 ttcctgtaga aacacttcca tttgcgatat tcccaatcac gggcatatca atagaaatgc
     3061 tctcggatat acttccggtc agttgagtga ctttttctct cgcaatcgaa caggctttac
     3121 ttattgctgc ttccttcgtt tttttaatta ttgaatccac agatggcagg ctgatgccga
     3181 ggtttcccac aggccaagcg tccgtacaac caatcgtctc aagtgactga ttgatgttat
     3241 ccatgaaatt cttcgctacc ggctctaact gagccgcatc ctgcttcatt gcgttatatt
     3301 gcgcttgttt taatgatgag ctgacatccg ggctacagac tgctgcgtta gctgtggttg
     3361 ctgaaattaa tattgttgcg ggaaggatta ttttgaaaat gcggacacag acatttgttc
     3421 gccatttatt tttat
//
LOCUS       DACBVI010000261         3386 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.265, whole genome shotgun sequence.
ACCESSION   DACBVI010000261 DACBVI010000000
VERSION     DACBVI010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.265"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            120..1085
                     /gene="dusB"
                     /locus_tag="IU325_22320"
     CDS             120..1085
                     /gene="dusB"
                     /locus_tag="IU325_22320"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP5811716.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1111..1407
                     /gene="fis"
                     /locus_tag="IU325_22325"
     CDS             1111..1407
                     /gene="fis"
                     /locus_tag="IU325_22325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HAP5811717.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1493..2377
                     /gene="yhdJ"
                     /locus_tag="IU325_22330"
     CDS             1493..2377
                     /gene="yhdJ"
                     /locus_tag="IU325_22330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAP5811718.1"
                     /translation="MRTGCEPTRFGNEAKTIIQGDALTELKKLPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            2461..2640
                     /locus_tag="IU325_22335"
     CDS             2461..2640
                     /locus_tag="IU325_22335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 domain-containing protein"
                     /protein_id="HAP5811719.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(2643..3305)
                     /gene="acrS"
                     /locus_tag="IU325_22340"
     CDS             complement(2643..3305)
                     /gene="acrS"
                     /locus_tag="IU325_22340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HAP5811720.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLWELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQNLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VI"
BASE COUNT          930 a          780 c          799 g          877 t
ORIGIN      
        1 ttttaaatgc aattctttga tccatctcag aggattggtc aaagtttggc ctttcatctc
       61 gtgcaaaaaa tgcgtaatat acgccgcctt gcagtcacag tatggtcatt tcttaactca
      121 tgcgcatcgg acaatatcag ctcagaaatc gcctgatcgc agcgcccatg gctggcatta
      181 cagacagacc ttttcggacg ttgtgctacg agatgggagc cggattgaca gtatccgaga
      241 tgatgtcttc taaccctcag gtttgggaaa gcgacaaatc tcgtttacgg atggtgcaca
      301 ttgatgaacc cggtattcgc accgtgcaaa ttgctggtag cgatccgaaa gaaatggcag
      361 atgcagcacg tattaacgtg gaaagcggtg cccagattat tgatatcaat atgggttgcc
      421 cggctaaaaa agtgaatcgc aagctcgcag gttcagccct cttgcagtac ccggatgtcg
      481 ttaaatcgat ccttaccgag gtcgtcaatg cagtggacgt tcctgttacc ctgaaaattc
      541 gcactggctg ggcaccggaa caccgtaact gcgaagagat tgcccaactg gctgaagact
      601 gtggcattca ggctctgacc attcatggcc gtacacgcgc ctgtttgttc aatggagaag
      661 ctgagtacga cagtattcgg gcagttaagc agaaagtttc cattccggtt atcgcgaatg
      721 gcgacattac tgacccgctt aaagccagag ctgtgctcga ctatacaggg gcggatgccc
      781 tgatgatagg ccgcgcagct cagggaagac cctggatctt tcgggaaatc cagcattatc
      841 tggacactgg ggagttgctg cccccgctgc ctttggcaga ggttaagcgc ttgctttgcg
      901 cgcacgttcg ggaactgcat gacttttatg gtccggcaaa agggtaccga attgcacgta
      961 aacacgtttc ctggtatctc caggaacacg ctccaaatga ccagtttcgg cgcacattca
     1021 acgccattga ggatgccagc gaacagctgg aggcgttgga ggcatacttc gaaaattttg
     1081 cgtaaacaga aataaagagc tgacagaact atgttcgaac aacgcgtaaa ttctgacgta
     1141 ctgaccgttt ctaccgttaa ctctcaggat caggtaaccc aaaaacccct gcgtgactcg
     1201 gttaaacagg cactgaagaa ctattttgct caactgaacg gtcaggatgt gaatgacctc
     1261 tatgagctgg tactggctga agtagaacag cccctgttgg acatggtgat gcaatacacc
     1321 cgtggtaacc agacccgtgc tgcgctgatg atgggcatca accgtggtac gctgcgtaaa
     1381 aaattgaaaa aatacggcat gaactaattc aggttagcta aatgcttgat taaaaaggcg
     1441 ctactcggca tggggaagcg ccttttttat aggtgtcaca aagggagtga ccatgagaac
     1501 aggatgtgaa ccgacccgat ttggtaatga agcaaagacc atcatccagg gtgatgcatt
     1561 aaccgaactt aaaaagctcc ccgccgaaag tgtcgatctg atctttgccg acccaccgta
     1621 taacatcggt aaaaattttg atggtctgat cgaagcctgg aaagaagatc tgtttatcga
     1681 ctggctgttt gaagtgattg cagagtgcca tcgcgttctg aaaaagcagg gcagcatgta
     1741 catcatgaat agcacggaaa acatgccctt tatcgatctc cagtgccgca agctttttac
     1801 catcaaaagt cgcatcgtct ggtcatatga cagttctgga gtgcaggcga aaaaacacta
     1861 cggctccatg tacgaaccca tcctgatgat ggtgaaagac gcaaagaact acacattcaa
     1921 cggtgatgct attctggtag aagccaaaac cggatcgcag cgcgcgttga tcgattatcg
     1981 caaaaaccct ccacagcctt acaatcatca aaaagtaccg ggtaacgtct gggattttcc
     2041 gcgcgtgcgt tatttgatgg atgaatatga aaaccacccg acgcaaaaac cggaagcctt
     2101 actgaaacgc attattctcg cctcttccaa cccaggcgat atcgttctcg acccgtttgc
     2161 tggtagcttt actaccggtg ccgtagccgt cgccagtggg cgaaaattca ttggtattga
     2221 gatcaacagc gagtacatca aaatggggct tcgacggctg gatgtcgcgt cgcattactc
     2281 tgcggaagaa ctggcgaaag tgaaaaaaag aaagaccggc aacctgtcaa aacgaagcag
     2341 gttaagcgaa gttgaccccg atctcattgc aaagtaaagg aagtgtaagc ctggttttca
     2401 gattattcat ttcgtgtata tttcctgcca gacttggtta aacatgcaca ggctcaggta
     2461 atgattcgca agtattggtg gctcgtcgtt ttcgctgtct tcgtttttct gtttgatact
     2521 ttactgatgc agtggattga actgctcgca acagaaacag acaaatgccg caatatgaac
     2581 tcagttaatc cactaaaact ggttaactgt gacgaactga attttcagga cagaatgtga
     2641 atttatatga cacttaattc attcgtttga tgaattaatt tcgttatgtt ttcatctggc
     2701 atgaacattc ttaatacgtt atcgaccagt gcgggtgctt gtttataaag atcataaccc
     2761 gccatattca ttaaccagtt ttgaacaatt ccgctgaagg caccatcaat aataatcatc
     2821 acaacatcta aatcgaggtt atttgctaca caaccttgtt gctgacacgc ctgcaatact
     2881 tcgcggagat tctgcggatt aaagcccatc ttttcgcgta tcactccctc ggccagcatc
     2941 tcatcattaa attcacattt gtgatataag attttcagca acgcctgctg gcggggaatt
     3001 ttggcaatat attgcaagcc gacaatcaat ttttcacgca attgttgaaa cgggtcatgc
     3061 tctaatccag ccgtcaagtg ttcctggatt aactcccaca atgaaggctg ttgcaaccac
     3121 atctcattaa acagttgagt cttgttttcg aagtgccagt agatagcgcc acgcgtaacg
     3181 ttagcggcgt cggcaatgtc gttgagcgtc gtcttgctta cgccatgctg cgcaaactgg
     3241 gcgatggcag tttcaatcag ttcttgccgg gtcttcaggg cttcagcttt ggttcttttt
     3301 gccatgatta attattcagg aaataaatat attcgacaca ggatgagaaa atagcgaagg
     3361 ttaatctatc acctaatgta tattta
//
LOCUS       DACBVI010000262         3347 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.266, whole genome shotgun sequence.
ACCESSION   DACBVI010000262 DACBVI010000000
VERSION     DACBVI010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.266"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            211..1731
                     /locus_tag="IU325_22345"
     CDS             211..1731
                     /locus_tag="IU325_22345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HAP5811721.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARKRQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(1757..2095)
                     /gene="maoP"
                     /locus_tag="IU325_22350"
     CDS             complement(1757..2095)
                     /gene="maoP"
                     /locus_tag="IU325_22350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HAP5811722.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            2214..3053
                     /gene="hdfR"
                     /locus_tag="IU325_22355"
     CDS             2214..3053
                     /gene="hdfR"
                     /locus_tag="IU325_22355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HAP5811723.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYKNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IATLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(3148..3223)
                     /locus_tag="IU325_22360"
     tRNA            complement(3148..3223)
                     /locus_tag="IU325_22360"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3188..3190),aa:Trp,seq:cca)
     gene            complement(3232..3308)
                     /locus_tag="IU325_22365"
     tRNA            complement(3232..3308)
                     /locus_tag="IU325_22365"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3272..3274),aa:Asp,seq:gtc)
BASE COUNT          894 a          831 c          819 g          803 t
ORIGIN      
        1 gtgagtgaaa aaaattcaat ttcagtatga atgttgttcc cttcctcgta gttcatcctt
       61 tgcccgctgc atttttgcga gcatcattcc accgccagaa caactcaact tcacttacac
      121 aaataaaccg gaatcagcca ctaaaattca cgaattagcg ggctacagca gcgatatctt
      181 atgcacataa tggtgactca aggagggctt atgtcactgt caattgttca tacccgcgca
      241 gccctgggag taaatgcgcc cccaatcact gttgaggtac atatcagtaa aggtctaccc
      301 ggcttaacga tggtgggctt accagaaaca acggtaaaag aagcccgcga tcgcgtgcgc
      361 agcgccatta tcaatagcgg atatgaatat ccggcgaaaa aaatcaccat caatctggct
      421 ccagccgatc tgccaaaaga agggggacga tatgatttac ctatcgccat tgcgttgctg
      481 gcggcctcag aacagcttac agccaataaa ttagatgaat atgaattagt cggagaactg
      541 gcgcttacag gcgctctgcg tggcgttccc ggcgcaatct ccagtgcaac tgaagctatt
      601 aagtcgggca gaaaaattat cgtcgcgaaa gataacgaag atgaagtggg gctaattaac
      661 ggtgaaggat gcctgatagc cgatcatctg caggctgtct gtgcgtttct ggagggtaag
      721 cacgctctcg aacgcccgaa accaactgat gcagtatccc gggcgctaca acatgatctc
      781 agtgatgttg tcggtcagga gcaaggaaag cgaggactgg aaattaccgc cgctggcggg
      841 cacaaccttt tactgattgg gccgccggga acaggtaaaa caatgctcgc cagccgtatt
      901 aatggcctgt tgccagattt aagcaatgaa gaggcactgg agagcgctgc gatattaagt
      961 ctggtaaatg ctgaatcagt acaaaaacaa tggcggcagc gcccgttccg ctcacctcat
     1021 cacagtgcgt cgttaactgc gatggtgggc ggtggtgcaa ttccagggcc aggtgaaatt
     1081 tcgctggcgc ataacggcgt gctttttctt gatgagctac ctgaatttga acggcgtaca
     1141 ctggatgcct tgcgagagcc gattgaatcc gggcagatcc atctttcacg cacacgagca
     1201 aaaataacct atccagcccg tttccaactt gttgcggcga tgaatcccag ccctaccgga
     1261 cattatcagg gaaaccataa ccgctgcaca ccagagcaaa cgctgcgtta tctcaaccga
     1321 ctctctggtc cctttctcga ccgcttcgat ctctcactgg agatcccatt accgccaccc
     1381 ggcattttga gtaaaacggt agtgccggga gaaagcagcg ccaccgttaa acaacgcgta
     1441 atggccgcca gaaagcgcca atttaagcgg cagaataaac tgaacgcctg gctggataat
     1501 ccggaaatac gccaattctg caagcttgag agcgaagatg cgcagtggtt ggaagaaacg
     1561 ctgatccatc tggggttatc gattcgtgcc tggcagcggt tattgaaagt tgcacgaacc
     1621 attgctgata ttgatcagtc cgacattatc acacgtcagc atttgcagga ggcagttagc
     1681 tatcgtgcca ttgaccgttt gctcatccat ctgcaaaaac tactgacata aaaaaaaggg
     1741 catttcgccc tttttattaa tcgtcagaat cggtgtagtc ttcagcacct tcaacctgcg
     1801 gtttaccgcc ggaaagggtg tgaaaacgtt ttggacgctt gatacgcgtc atatacttgg
     1861 accacacgcg ttctgcttct gtcactggct cacgttcgcc acggcatact gctacgaaga
     1921 gtttctcttc ctcggtaacc ggctcgcgtt tgccaagatc caactcattg aaggcataac
     1981 catgacgctc aagcagttgt gcctctttga tggtgaagtc accatgacga gagaatccac
     2041 gtggataatg tttattgtcg aaatatcgat tagtcgtcgt aaagctttcc gccatcctgc
     2101 acgctcctaa ttctttgacc gagctagtta tggcgcggag tattagttac gcttgacaga
     2161 gtgtaaaaca aaacatttaa atcataacga caaataattt tgcggagagc actgtggata
     2221 cggaattgtt aaaaactttc ctggaagtta gccgaacgcg tcactttggt cgagcggctg
     2281 aatcgctcta tctgacccaa tcagccgtga gctttcgaat cagacaactg gaaaatcaac
     2341 tgggtgtgaa tcttttcacc cgccacagaa acaatatccg tttaaccgcc gccggagaaa
     2401 agcttctgcc ttatgcagaa acgctcatga gcacctggca ggccgcccgt aaggaggtgg
     2461 cgcatacctc acgacataac gagttttcta tcggtgccag cgcctcattg tgggaatgta
     2521 tgcttaatca gtggctggga cgtttgtata aaaatcagga tgcccataca ggcttacagt
     2581 tcgaagcgcg aattgcccaa cggcagtctc tggtaaaaca gctgcatgaa cgccagcttg
     2641 atcttcttat tactactgaa gcgcccaaaa tggacgaatt tagcagtcag ttgctcgggt
     2701 atttcacttt agcgctttat accagtgccc cttccaaact aaagggagat cttaactatc
     2761 tgcgactgga atgggggcca gattttcaac agcatgaggc aggtttgatc ggtgctgacg
     2821 aagttcccat tctgacaacc agttctgctg aactggcaca gcaacagatt gcgacgctta
     2881 atggttgcac ctggctaccc gtcagttggg cgcgtaaaaa gggcggcctg cataccgttg
     2941 tcgatagcac aacactttca cggccgcttt atgccatatg gctgcaaaat agcgataaaa
     3001 atgcgttgat tcgcgatcta ttgaaaatta acgtgctgga tgaagtgtat taatctgaat
     3061 ggctggcaag gatgccggta gaaggattta cttcggagag gttatttcag ataaaaaaaa
     3121 tccttagctt tcgctaagga tgatttctgg caggggcgga gagactcgaa ctcccaacac
     3181 ccggttttgg agaccggtgc tctaccaatt gaactacgcc cctaattagg gtggcggaac
     3241 ggacgggact cgaacccgcg accccctgcg tgacaggcag gtattctaac cgactgaact
     3301 accgctccac cgaattcttt tacaaccacc ggttttatga ccggctt
//
LOCUS       DACBVI010000263         3281 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.267, whole genome shotgun sequence.
ACCESSION   DACBVI010000263 DACBVI010000000
VERSION     DACBVI010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.267"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(88..615)
                     /gene="mobB"
                     /locus_tag="IU325_22370"
     CDS             complement(88..615)
                     /gene="mobB"
                     /locus_tag="IU325_22370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HAP5811724.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVSLNLDVALLDINDVG
                     GLADFVVEWMQKQNG"
     gene            complement(597..1181)
                     /gene="mobA"
                     /locus_tag="IU325_22375"
     CDS             complement(597..1181)
                     /gene="mobA"
                     /locus_tag="IU325_22375"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HAP5811725.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQETGEWFLFCPCDT
                     PYIPPDLAARLNHQRKEAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            1251..1520
                     /locus_tag="IU325_22380"
     CDS             1251..1520
                     /locus_tag="IU325_22380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1040 family protein"
                     /protein_id="HAP5811726.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1597..2583
                     /locus_tag="IU325_22385"
     CDS             1597..2583
                     /locus_tag="IU325_22385"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HAP5811727.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAVQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2600..3226
                     /gene="dsbA"
                     /locus_tag="IU325_22390"
     CDS             2600..3226
                     /gene="dsbA"
                     /locus_tag="IU325_22390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HAP5811728.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          827 a          786 c          796 g          872 t
ORIGIN      
        1 aaaggccatt cgaaaggatg gcctttttgc gttggtgaaa aaaatgccgg atgcgacgct
       61 ggcgcgtctt atccagccta ctcttgctta tccgttctgc ttttgcatcc actccaccac
      121 aaaatcagcc agccccccaa catcattaat atccagtaac gcgacatcaa gattaagtga
      181 cacatcactg gctacagcaa taacatgtct gtctatcact aattcttccg gtcgatgtcc
      241 ggctccatcg cgaaacagca caatctttgc gatctcttca tgcttaaacc cttcgaccag
      301 aatcaaatcc agctttgagg tatccattcg acttgcgaga aaatgtagat ccagctcttc
      361 ttcgtctggt gtttccgtca tcaaggccca tcgctgctgg ctggcaacga tggtttgtgc
      421 cgcgccagcc tttcgcagct catagctatc tttgcccggt ttatcaacat ccatatcatg
      481 gtgcgtatgc ttaatcagcc ctggacggat ccctctggcg cataatgccg ggatcagttt
      541 tttcaaaagc gtagtttttc cagtgccgct ccacgcggca aaggcgagta acggtatcat
      601 cgtttttcct gccatcgggc aagctcctct ggcgtattca cgttaataaa tgcatcttta
      661 ttatcgctga aatcaaccgc atgaccgcca gccagacgca taaatgccat tactcggcgt
      721 tctcctgctt gcagatattc cagtaataaa ggctcaattg cgcggtttac cagagcaatc
      781 gtcgggtgat cgcgttcacc gtcgtggacc cacacgacag gtgcctcttt gcgctgatgg
      841 ttaagccggg ctgctaaatc agggggaatg taaggcgtat cgcacggaca aaacaaaaac
      901 cactcccccg tttcctgctg cattactgaa agcattcctg ccagagggcc tgggtaatcc
      961 gccagtgaat cttcaatcac tttcagaccg cttgcctgat agatttcctg atgacgattg
     1021 gcattaacca cgacgtgcga gagctgcgtc ataagcgcgt cagcgacatg ttgccacagc
     1081 ggtttgccgt ttaattcaag caatcctttg tctacgccgc ccattcgtct ggctttaccg
     1141 cccgccagca caacgcctgt tatcgtcgtc atcagattca ccgatatcgc ctcttttatt
     1201 gtgggattga ccctgctaac gtgtctgtct caagagtaag gagcattact atgaaatgta
     1261 aacgtctgaa tgaagttatt gaactcctcc agccagcctg gcaaaaagag ccagacctta
     1321 acctgctgca atttttgcag aaactggcga aagagtcagg ttttgacggc gaactggcgg
     1381 atttgacgga tgacattctg atctatcatc tgaaaatgcg tgattccgcg aaagatgcgg
     1441 ttatcccggg tttgcaaaaa gattatgaag aagatttcaa aacggcgctg ttacgcgctc
     1501 gcggcgtaat taaagagtaa aagcttgtaa gcggcgccac caaaatcatc gtgaaatgat
     1561 atccttcatc attcgtaatg ttttccggat gatgggatga acaacagcgc ttttactttc
     1621 cagacactac acccggatac catcatggat gctctatttg agcaagggat ccgggtggat
     1681 tccggtctta ccccgcttaa cagctatgaa aaccgtgtct atcaatttca ggacgaagat
     1741 cgtcgacgtt ttgtcgtcaa attttatcgc cctgaacgtt ggacagccga ccaaatcctc
     1801 gaagaacatc agtttgcgtt gcagctggta aatgatgaag ttccggtcgc agcacctgtg
     1861 gcctttaacg gtcagacttt attgaatcat cagggatttt atttcgctgt ttttccaagc
     1921 gtcggtggtc gccagttcga agctgataat atcgatcaga tggaagcggt tgggcgttat
     1981 ttagggcgta tgcaccagac ggggcgcaaa cagcttttta tccatcgccc gaccatcggt
     2041 ctgaatgaat atctcattga gcctcgcaag ctgtttgagg acgctacact gataccttcc
     2101 gggttgaaag cggcatttct gaaagcgaca gatgagctga tcgccgccgt tacagcacac
     2161 tggcgggaag atttcaccgt tctgcggcta catggagact gccacgctgg gaatattctc
     2221 tggcgcgatg gtccaatgtt tgttgatctg gatgatgcac gtaatggtcc agccgttcag
     2281 gatttgtgga tgttgctcaa tggcgataaa gccgagcagc ggatgcaact ggaaactatt
     2341 attgaagctt atgaagaatt tagcgagttc gacaccgctg aaatcggact gattgaacct
     2401 ttacgcgcca tgcgtttggt ttattatctt gcctggctaa tgcggcgttg ggctgatccc
     2461 gcgttcccga aaaatttccc gtggttaacc ggggaagatt actggctgcg acagacggcg
     2521 acttttatag aacaggcaaa agttctacaa gaaccccctt tgcaattaac acctatgtat
     2581 taatcggaga gagtagatca tgaaaaagat ttggctggcg ctggctggtt tagttttagc
     2641 gtttagcgca tcggcggcgc agtatgaaga tggtaaacag tacactaccc tggaaaaacc
     2701 ggtagctggc gcgccgcaag tgctggagtt tttctctttc ttctgcccgc actgctatca
     2761 gtttgaagaa gttctgcata tttctgataa cgtgaagaaa aaactgccgg aaggcgtgaa
     2821 gatgactaaa taccacgtca acttcatggg tggtgatctg ggcaaagatc tgactcaggc
     2881 atgggcagtg gcgatggcac tgggcgtgga agacaaagtc actgttccgc tgtttgaagg
     2941 cgtacagaaa acccagacta ttcgttcagc atctgatatc cgcgatgtat ttatcaacgc
     3001 aggtattaaa ggtgaagagt acgacgcggc atggaacagc ttcgtggtga aatccctggt
     3061 cgctcagcag gaaaaagctg cagctgacgt gcaattgcgt ggcgttccgg cgatgtttgt
     3121 taacggtaaa tatcagctga atccgcaggg tatggatacc agcaatatgg atgtttttgt
     3181 tcagcagtat gctgatactg tgaaatatct gtccgagaaa aaataattca atgtaaatta
     3241 cataaagtcc gtgaatattc acgggctttt tttattattt a
//
LOCUS       DACBVI010000264         3236 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.268, whole genome shotgun sequence.
ACCESSION   DACBVI010000264 DACBVI010000000
VERSION     DACBVI010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.268"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..368
                     /locus_tag="IU325_22395"
     CDS             <1..368
                     /locus_tag="IU325_22395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP5811729.1"
                     /translation="KDILFYLNNNYREKITLEQLSKKFRASVSYICHEFTKEYRISPI
                     NYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKN
                     CFAEQEILSEFPQPVSLAG"
     gene            complement(411..1562)
                     /locus_tag="IU325_22400"
     CDS             complement(411..1562)
                     /locus_tag="IU325_22400"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAP5811730.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPEPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1576..3171)
                     /locus_tag="IU325_22405"
     CDS             complement(1576..3171)
                     /locus_tag="IU325_22405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase YdiF"
                     /protein_id="HAP5811731.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNQEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIVFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
BASE COUNT          796 a          821 c          748 g          871 t
ORIGIN      
        1 taaaagatat tcttttctat ctgaataata actatcgcga aaaaatcact ttagagcagt
       61 tatcgaaaaa gtttcgtgcc agcgtcagtt atatttgcca tgaatttacc aaagagtatc
      121 gtatttcccc tattaactat gttattcaac ggcgtatgac ggaagcgaaa tggtcactca
      181 ctaatactga attatcacag gcagagatct cctggcgtgt gggttatgaa aatgtcgatc
      241 actttgccaa actgtttttg cgccatgtcg gctgttcgcc cagcgattac cgcaggcaat
      301 ttaaaaactg ttttgcggaa caagaaatct tatctgaatt tcctcaaccg gtaagtcttg
      361 ccggataaat ctggcggcgc agtttgttaa gaagctgcgc cgcctgcaga ttatttgttc
      421 tgataatctt tcaggatctg ccgacccgct acgtaaatca taatttcgtc tgtaccgccg
      481 ccgatacgtt cacaacggac atcacgccag aagcgagaga cgcgcgcctc atcggtatag
      541 cccagaccgc ccatgatttg aatcgcatca tcaataactt ccattgcggt acgtgcgcag
      601 tacagttttg ccagcgccgc gctggtgcgc agtgactgat gctgatcggc ttgccatgcc
      661 actttcagca ccatgttgcg catgttgtcg atcttaatcg ccatcagcgc cagtttttcc
      721 tggatcatct ggttatgacc aatgggctta ccaaaagcga tacgttggtt ggcatagcgg
      781 gcggcatctt caaaggcaca ctcggcaaag ccggtgctgc gcgcggcgtt gatcaggcgc
      841 tccatctcaa agttgtacat cacattgagg aaacccattc cttcttcgcc caccatgtcg
      901 ctctcttcaa cttccacgtt gtcgagatag acttcgcagg tgctgagcat atgccagccg
      961 attttatgca gcgggttaat cttaatgccg ggcttactgg agtcgaccca ccacagggtg
     1021 aaggcttttt tgggatcttt cggttctgga tcgcgcgcca gcaccagcat atacgggtac
     1081 tctttcgcgc cggtaataaa ggttttctgg ccgttgatgt aaaccttgcc gtttttacgc
     1141 gtgtaagtgg tggtggcgct gttgttatct gagcctgcgc ctggttccgt caatgccagg
     1201 gcataggcgg gatcacccgt ttccagggtg ctctctgccg ttttacgtag ctgctctgca
     1261 gaaccgaaac ggcgcatact gtgaatacat tgaccgttgg tgatcaaaaa tgccggggca
     1321 ccgcattttg acacttccat cagcgccagc atttgggtga cgtaatccgc agggatacca
     1381 ccaaattctt ccggcacgcc aagcatggaa ataccgttat ccgccagtgc ccgcataaat
     1441 tcacgcgggt atgtcccgtt ttgatcgcag gtgcggaaat actcttccgg aaagttagtc
     1501 gtaatcagtt cgcgaatact ggccagcagc agttcttgtt cttcagttaa agaaaaatcc
     1561 atcgtgatac tcctgttaat gagccgcttc aggcaggaca aaacccatcg ccgcatcgat
     1621 aaataatctt tcgtccatca gtttgagttc tggcgaaatc actggggtga aatccatttt
     1681 gtcgagaata tctttttgta aatcgacgcc aggggcgatt tcgattaaat gcaggccgtc
     1741 ttctttcagc gtgaatactg cgcgctcagt gatataacga acatccagcc ctcgctcgag
     1801 agcgattttt ccgctgaaag taatttccgg tagttcccga ataaatttct tcacccgtcc
     1861 ttcctggacg atatttaatt tgccgtcggt aatttctgtt tttaaactgc ccgcagttaa
     1921 tgtgccgcag aaaacgattt tcttcgaagt ggcactgata tcaataaatc caccggtgcc
     1981 catgatttta ccattgaatt tatgcacgcc gacgttaccg tgctggtcga cttcagcaaa
     2041 actcaaataa caaacatcca gaccgccacc gtgataaaaa tcaaactggg acgtcatatc
     2101 cagaatggca cgggtattca cgttcgcgcc aaaggcgatc ccctgtgaag taattccgcc
     2161 aatcggacct gtttctaccg tcagaataaa gtcatcagca caaccttctt ctcgggcgac
     2221 caggccaatg ccgtcagcaa taccgacgcc gacattcccc accgcgcctt tacgcatttc
     2281 gaataatgcg cgccgtgcaa ctaatttacg ttggtttagg ggcaggctaa gtttggtgct
     2341 gtcatcaagg gtgaagtcac cagaaataaa gcggttaacc ggcgcaccgc catacagttg
     2401 ggtttgatcc ggatcgacca ccacaatatc caccagataa cccggaatac ggacagattt
     2461 aggatgcagc gtggctttct taaccatttt ctgcacctgc atcatcacaa taccgccgtt
     2521 attgtgcacc gcctgggcaa taaccaatgc gtcgagatac atcacctcat cttcaaaagt
     2581 ggcgtagcct tcgctgtcgc aggtggtagc gcgaatgaag gcgatatctg gcgcaatcgc
     2641 tttgtaatag agatattctt ggttatcaaa ctcgaccagt ttaatcaggt cttctttagt
     2701 gacttcattc agtttgccgc cttgctggcg tggatcaaca aatgtcccga tgccaatatc
     2761 actaataata ccaggctggt gggctgcggc ggcgcgtaag gtttgtgtaa gtacaccttg
     2821 tggatagtta taagcaataa ttttattttg ttctgcgaga tcagaaatac gcggcgattg
     2881 tccccagtga ccgcataatg cccatttcac cagaccttct tgtgccagcg gactaatacc
     2941 acggtcggcg cgatcgccaa gccctgttgg actaataatc gataaattac gtggtgtttg
     3001 agtctgttta tatttatcag caagagcagt aattaacgtg gtggcttcca gaataccgcc
     3061 gccagcgcct aacacacaaa gtgttgcttc gtcgggaata taattcaccg cttcctgtgc
     3121 cgacaggacc ggcactcgtc cattaatacg aggtggtttt acaggtttca ttgttaattc
     3181 ctgattcaga tgtgattttc cctacgccag atggagaatt agcgcacaga gactca
//
LOCUS       DACBVI010000265         3206 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.269, whole genome shotgun sequence.
ACCESSION   DACBVI010000265 DACBVI010000000
VERSION     DACBVI010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.269"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..732)
                     /locus_tag="IU325_22410"
     CDS             complement(<1..732)
                     /locus_tag="IU325_22410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopN family type III secretion system gatekeeper
                     subunit"
                     /protein_id="HAP5811732.1"
                     /translation="MAIHVEHVGVLERAREVSRLEDIITEDNEDIEAEMPKMGDDPAG
                     KEARFLQATDEMSAALTQFMKKKIYEEQLANFLDGEEYVLEDQPIEKTDKVMEALKAA
                     TTHDYEVYSFAKKLFPDESDLVVVLRAILRKKQISENVRLNAEALLKKVNQETTKKFI
                     NSGINSALKAKLFGQALSLNPKLLRASYRQFLMAEDDAVDTYVEWIGSYGYQNRMLVT
                     KFIKETLFSDINALDASCSSLEFGMF"
     gene            complement(737..2440)
                     /locus_tag="IU325_22415"
     CDS             complement(737..2440)
                     /locus_tag="IU325_22415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscC/YscC/HrcC family type III secretion system
                     outer membrane ring protein"
                     /protein_id="HAP5811733.1"
                     /translation="MKIKLRITIILISALCIFNGLLTPGAYAATANGYVANKENLRSF
                     FETVSSYAGKPTIVSKLAMKKQISGNFDLTEPYALIERLSAQMGLIWYDDGKAIYIYD
                     SSEMRNALINLRKVSTNEFNNFLKKSGLYNSRYEIKGGGNGTFYVSGPPVYVDLVVNA
                     AKLMEQNSDGIEIGRNKVGIIHLVNTFVNDRTYELRGEKIVIPGMAKVLSTLLNNNIK
                     QSTGVNVLSEISSRQQLKNVSRMPPFPGAEEDDDLQVEKIISTAGAPETDDIQIIAYP
                     DTNSLLVKGTVSQVDFIEKLVATLDIPKRHIELSLWIIDIDKTDLEQLGADWSGTIKI
                     GSSLSASFNNSGSISTLDGTQFIATIQALAQKRRAAVVARPVVLTQENIPAIFDNNRT
                     FYTKLVGERTAELDEVTYGTMISVLPRFASRNQIELLLNIEDGNEINSDKTNVDDLPQ
                     VGRTLISTIARVPQGKSLLIGGYTRDTNTYESRKIPILGSIPFIGKLFGYEGTNANNI
                     VRVFLIEPREIDERMMNNANEAAVDARAITQQMAKNKEINDELLQKWIKTYLNREVVG
                     G"
     gene            complement(2437..3186)
                     /locus_tag="IU325_22420"
     CDS             complement(2437..3186)
                     /locus_tag="IU325_22420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP5811734.1"
                     /translation="MIEEGLLLPMNVLLQGQKLTLLDPEMWFLRGNENKDVTLVITIG
                     NNHQKLMVVEDILLLIDRSQIEVTAGKVIYHPLRIDILSKLLAFIDESTGSVERERHE
                     LFADALPFASEVVLFSKVASEAWFLATYLSSDNINEILFQHLRKTECYKLVRYLLSQS
                     LIQTSLYDLGELYGVSYSHFRRLCSYALGGKVKTELCGWRVARAVLEIIEGNSDMTTI
                     AHKYGYSSSSHFSAEVKSRLGKTPRELCKKL"
BASE COUNT          910 a          705 c          527 g         1064 t
ORIGIN      
        1 aaacatacca aattccaatg atgaacagct cgcatcaagt gcatttatat ctgagaaaag
       61 cgtctctttg ataaattttg tcaccagcat tctattttga tagccataac taccaatcca
      121 ttcgacataa gtatctacag catcgtcttc tgccattaaa aattgtcgat aacttgccct
      181 tagtaatttt ggatttaatg acaacgcctg gccaaataac tttgctttta atgccgaatt
      241 aataccgcta ttaatgaatt ttttagtagt ctcttgatta acttttttaa gtaatgcttc
      301 agcatttagc cgcacatttt cagatatttg ttttttacgt aaaattgcac gtaatacaac
      361 caccagatcg ctttcatctg gaaataattt tttagcaaag ctatacactt catagtcatg
      421 ggtagttgct gccttaagcg cctccatgac tttatcggtt ttttctattg gctgatcttc
      481 caggacatat tcttctccgt ccagaaagtt ggccagttgt tcttcgtaga tttttttctt
      541 cataaactgc gttaacgctg ctgacatctc gtcagtagct tgtaagaatc gagcttcttt
      601 accggcggga tcatctccca ttttaggcat ttcagcttca atatcttcat tatcttctgt
      661 gataatgtcc tctaagcgag aaacttctct ggctctttct aatacaccga catgttctac
      721 atgaatcgcc atttcactat cctccgacga cttcacgatt caggtaagtt tttatccatt
      781 tctgcagtaa ttcatcgttg atttctttat tttttgccat ttgctgtgtt attgctctgg
      841 cgtcaaccgc agcctcattc gcattattca tcatgcgctc atcgatctct cttggctcaa
      901 taagaaagac acgaacgatg ttattcgcat ttgttccttc atatccaaat aatttaccaa
      961 taaatggaat actgcctaat attgggatct tgcgactttc ataggtattc gtatctcgtg
     1021 tatatccacc aatcaaaaga cttttccctt gaggcacgcg cgcaatagta cttataagcg
     1081 ttctaccaac ctgaggcaga tcatcaacat tggtcttgtc agaattgatt tcattgccat
     1141 cttcaatatt caagagtaat tcaatttgat ttcgcgaagc aaatcgtggt agcacgctaa
     1201 tcatcgtacc ataggttact tcatctaact ccgcagtacg ctcgccgact aatttagtat
     1261 agaaagtacg gttattatca aatatagcag gaatattctc ctgtgttaaa acaacaggac
     1321 gagcaacaac ggcggctctt cttttttgcg caagtgcctg tattgttgca ataaactgtg
     1381 tcccatccag ggtactaatt gaacctgaat tattaaatga tgcacttaag ctggatccaa
     1441 tttttatcgt gcccgaccag tcagctccta actgttctag atcagtctta tcaatatcta
     1501 ttatccataa agataattca atgtgacgct ttggaatatc gagtgtagct actaacttct
     1561 cgataaaatc aacctgagat actgttcctt taacaagtaa actgttggta tcaggatatg
     1621 caataatttg tatatcgtca gtctctggcg ctccagctgt cgatattatt ttctctactt
     1681 gtagatcatc atcttcttca gcaccaggaa aaggaggcat acgtgatacg ttttttagtt
     1741 gttgacgact tgaaatttca gaaagtacgt taaccccagt gctttgctta atgttattat
     1801 ttaacaacgt cgatagtact tttgccatgc caggaataac tatcttttcg ccacgtaatt
     1861 cataagtccg atcattaacg aatgtgttga ccaggtgaat aatccctact ttattgcgtc
     1921 cgatctcgat gccatcactg ttttgctcca ttaattttgc agcattaacg accaggtcaa
     1981 catacaccgg tggccctgaa acatagaaag taccattgcc gcccccttta atttcataac
     2041 gagagttata cagacccgat ttttttaaaa aattattaaa ctcattcgtc gatacctttc
     2101 tcagattaat cagcgcatta cgcatctctg atgagtcata gatataaata gctttgccat
     2161 catcgtacca aataagtccc atctgcgccg acaggcgttc aatcaaggca tagggttcgg
     2221 ttaaatcaaa atttccgctg atctgtttct tcatggcaag cttacttact attgtaggct
     2281 tacccgcata tgatgaaacc gtttcaaaaa aactacgtag attttcttta tttgctacgt
     2341 atccattcgc tgttgcggca tatgcaccag gagtcaataa tccattaaaa atgcataagg
     2401 ctgaaattaa tataatagta atacgtaatt ttattttcat aactttttac atagttctct
     2461 cggtgttttt cctaaccggc tttttacttc ggctgaaaag tgtgatgagg atgagtagcc
     2521 atatttatgc gcaatagttg tcatatcact gtttccttca atgatttcca acacggctct
     2581 ggcaactctc cagccacata attccgtttt aactttacca cccaatgcat aactacataa
     2641 acggcgaaaa tgtgaatacg atacgccgta aagctcacca agatcataga gcgaagtctg
     2701 tatgagcgat tgcgataata aatagcgaac cagtttatag cattctgttt ttcttagatg
     2761 ctgaaacaga atttcattaa tattatcgct gctcagataa gtggctaaaa accaggcttc
     2821 acttgcaact ttactgaata gcacaacttc tgatgcaaag ggcaaggcat cagcaaacaa
     2881 ctcatggcgt tccctttcca cactccccgt tgactcatca ataaaagcta ataatttact
     2941 caggatatca atgcgaagtg gatgataaat tactttccct gcagttacct caatttggct
     3001 tcggtcaatg agcagtaata tgtcttccac taccattaat ttttgatggt tattaccaat
     3061 cgtaataaca agggttacat ccttgttttc atttccccgt aagaaccaca tttcagggtc
     3121 cagtagggtt aacttctgtc cttgaagcaa aacattcatt ggaagtaaca gcccttcttc
     3181 aatcatgttt catctcctta ttccat
//
LOCUS       DACBVI010000266         3188 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.270, whole genome shotgun sequence.
ACCESSION   DACBVI010000266 DACBVI010000000
VERSION     DACBVI010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.270"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            348..1031
                     /locus_tag="IU325_22425"
     CDS             348..1031
                     /locus_tag="IU325_22425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAP5811735.1"
                     /translation="MNLKKTLLSVLIIVPLCLLAGCDYIEKASKVDDLVTQQELQKSK
                     IEALEKQQELDKRKIEHLEKQQTTVINSTKTLAGVVKAVKDKQDEFVFTEFNPAQTQY
                     FILNNGSVGLAGRVLAIDAVENGSVIRISLVNLLSVPVSNIGFHATWGNERPTDAKAL
                     AKWQQLLFNTTMNSTLQLMPGQWQDINLTLKGVSPNNLKYLKLSINMANLQFDTVQPA
                     ETRQRKNKK"
     gene            1075..>3188
                     /locus_tag="IU325_22430"
     CDS             1075..>3188
                     /locus_tag="IU325_22430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811736.1"
                     /translation="MNKIFKVIWNPATGSYTVASETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGVQAQAGRDNGQGVNYGQGTGTGWVAIGEDAKANSFTDTGGGSSTAVGYHSTA
                     DGRWSTALGAKTHSLGEASVALGINTTSAGERSLAIGASATSTGGFSIALGRYANSVG
                     EFSIAQGDHAETGADDAIAFGRESKALGIMSIALGATANASKEYAMALGASSAASAAN
                     AIAVGRNSAAAGVDSLAFGRLSAANAANAIAMGAESKAAENATAVGTNAEANGLNSIA
                     LGSGSIADVDNTIALGNQSQAVAAGAIAIGQGNKADGANAIALGNGSITGGVNAIALG
                     QGSYAGLENGTAIGAQASAQGKNSVALGAGSVATDADTVSVGNTTAQRQIVNMAAGDI
                     STTSTDAINGSQLYAISKSVADNLGGGATVNAQGVVTSPNYRLKSGIFGTVGDALTGL
                     DNNTLQWDSLKKAYSAAHGTDTTSTITNVKDGAISDTSKDAVNGSQLKTTNDNVATNT
                     ANITTNTNSINTLTDSVGDLKDDALLWNGTAFSAAHGTEATSKITNVKDGDLTAGSTD
                     AVNGSQLKTTNDNVATNTTNITNLTDSVGDLKDDALLWNGTAFSAAHGTDATSKITNV
                     KDGDLTAGSTDAVNGSQLKTTNDAVAANTTNIATNTTNITNLTDAVDSLGDDSLLWNA
                     TAGAFSAAHGTDATSKITNVTAG"
BASE COUNT          940 a          760 c          768 g          720 t
ORIGIN      
        1 catatcaatg cttgaaacat aattaattat ttaaattttt ctatcagcat cgcaaaattc
       61 gcaataaaac atcactcaac tgaatattta tctttccctg ccccgggcct ttatccggtg
      121 aaacatttga cattgattga caccgatcat taaaatccgc gcgatcaatt tatttattgg
      181 ctacaaacta aaattgtttg acacttaaaa acatattcgc acaacccatt aatacaaaaa
      241 ttctctaaca aatttatttg gcggatatgc cccactctaa tcattcataa aaaaatgatt
      301 gtactgtaat tatcaacgta tcgggattat gaaaaggaat tcatcctatg aacctaaaga
      361 aaacactgtt aagcgtgtta ataatagtgc ctctctgctt attggcaggg tgtgactata
      421 ttgaaaaagc gagtaaggtc gacgacctcg ttacacagca agagttgcaa aaaagcaaaa
      481 ttgaggcgct tgagaaacag caggaactcg ataagcgaaa gatagaacat cttgaaaaac
      541 aacaaactac tgtcataaat agtaccaaga cgctcgctgg cgtggtgaaa gcggttaaag
      601 acaagcagga cgaatttgtc tttacagaat ttaacccagc acaaacacaa tatttcattt
      661 taaataacgg atcggtcggt ttggcaggcc gggtgctggc tattgatgcg gttgaaaatg
      721 gcagtgttat tcgtatttcg ctggttaacc ttttaagcgt tcccgtatca aatattggct
      781 ttcatgcgac atggggcaat gaaagaccca ccgatgccaa agctctggca aagtggcaac
      841 aattactttt caacaccacc atgaattcca ctctgcaatt aatgcctggt caatggcaag
      901 acattaatct gacattaaaa ggcgtttcgc ctaataacct gaagtatctg aagttgtcca
      961 tcaatatggc caatctccag ttcgacactg ttcaacctgc tgaaactcgg cagcgaaaaa
     1021 acaaaaaata aatgcagctt ctgcgcttat atcaaggaat agagagcatc aataatgaac
     1081 aaaattttta aagttatctg gaatccggca acaggcagtt acaccgttgc cagtgaaacg
     1141 gcaaaaagcc gtggcaagaa atccgggcgc agtaagctgt taatttctgc cctggttgct
     1201 gggggaatgt tgtcgtcgtt tggggtacag gctcaggcag gaagggacaa cggtcagggc
     1261 gtcaattatg gccagggaac aggaacaggc tgggttgcta tcggtgaaga tgcaaaagca
     1321 aatagtttta cagacaccgg gggtggctcc agcaccgcag taggttatca ctcaactgct
     1381 gacggaaggt ggtcaacagc cctcggtgct aagacccatt cgcttggcga agcatcagtg
     1441 gcgttaggga ttaacactac ctcagcaggt gagcgaagcc tcgctatagg tgcatcggca
     1501 acgtcaacgg gtggattttc aatcgcactt ggtcgttatg cgaactcagt aggtgaattt
     1561 tctattgcac aaggggatca cgcagagact ggtgctgatg acgccatcgc atttggacgt
     1621 gaaagcaagg ccttaggaat tatgagtatc gccctcggag ccacggctaa tgcgtcgaaa
     1681 gaatatgcaa tggcgctggg tgcaagtagc gccgcatctg cagctaacgc gattgcagta
     1741 ggccgcaaca gtgctgcagc tggcgtagac agcctcgcat tcggtagact atccgccgcc
     1801 aacgccgcaa acgcgattgc tatgggtgca gagagcaaag ctgctgaaaa tgcgacggca
     1861 gttggtacta acgcggaggc aaatggactt aatagtatcg cgttgggctc cggcagtatt
     1921 gcagatgtcg acaacacgat tgctctgggc aatcaaagtc aggcagtagc ggctggcgca
     1981 attgccatcg gtcaagggaa taaagctgac ggcgcaaatg ctatcgcgct gggtaatggt
     2041 agtattacag gtggtgtaaa tgctattgct cttggacaag gcagttatgc cggtttagaa
     2101 aatggcactg caattggtgc tcaagccagt gctcaaggga aaaattcagt tgctctgggt
     2161 gctggttctg tagcgactga cgcggatact gtttctgtgg gtaacacaac agctcagcga
     2221 caaattgtca atatggcagc aggtgatatc agcactacca gtactgatgc catcaatgga
     2281 tcacagcttt atgctatcag taagtcagta gcggacaatc ttggtggggg ggctaccgtc
     2341 aatgcgcaag gcgtcgttac ttccccaaat tacaggctga aaagtggtat tttcggcact
     2401 gttggcgacg ccttaacggg cctggacaat aatacgttac aatgggactc ccttaaaaag
     2461 gcatatagtg cggcacatgg tacagatact accagtacca tcaccaacgt taaagacggc
     2521 gctatttctg ataccagtaa ggatgcggtt aacggttctc agctgaaaac caccaacgat
     2581 aacgtagcga ccaatactgc caatatcacc accaacacta acagtatcaa taccctgacg
     2641 gattccgttg gcgaccttaa agacgatgcc ctgctgtgga atggcaccgc gttcagcgcc
     2701 gcgcacggca ccgaagccac cagcaaaatc accaacgtca aagacggtga cctgacggct
     2761 ggcagcaccg acgcggttaa cggctctcag ctcaaaacta ctaacgataa tgtggcaacc
     2821 aacaccacca acatcaccaa cctgacggat tccgttggcg accttaaaga cgatgccctg
     2881 ctgtggaatg gcaccgcgtt cagcgccgcg cacggtaccg atgccaccag caaaatcacc
     2941 aacgtcaaag acggtgacct gacggctggt agcactgacg cggtaaacgg ctctcagctg
     3001 aaaaccacta acgatgccgt ggcagccaac accaccaata tcgccacgaa taccaccaac
     3061 atcaccaacc tgactgacgc agttgacagc ctcggtgacg attccctgct gtggaacgct
     3121 acagcggggg cattcagcgc cgcacacggt actgatgcca ccagcaaaat caccaacgtc
     3181 accgctgg
//
LOCUS       DACBVI010000267         3158 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.271, whole genome shotgun sequence.
ACCESSION   DACBVI010000267 DACBVI010000000
VERSION     DACBVI010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.271"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            31..1173
                     /locus_tag="IU325_22435"
     CDS             31..1173
                     /locus_tag="IU325_22435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HAP5811737.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGVELHGAHGFLLQNFFSPLFNQRNDRWGGDLERRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESATGGLRIEDTYKLLDRLIASGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISTGLPLVAIGKGLVINPEWV
                     TLAESGRSHEIQTTLNPQLVPELTIPDKLWDQIQASKGTGWFPLMD"
     gene            1645..1746
                     /locus_tag="IU325_22440"
     CDS             1645..1746
                     /locus_tag="IU325_22440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811738.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            2105..2368
                     /locus_tag="IU325_22445"
     CDS             2105..2368
                     /locus_tag="IU325_22445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP5811739.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            2368..2508
                     /gene="rpmJ"
                     /locus_tag="IU325_22450"
     CDS             2368..2508
                     /gene="rpmJ"
                     /locus_tag="IU325_22450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP5811740.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(2578..2768)
                     /locus_tag="IU325_22455"
                     /pseudo
     CDS             complement(2578..2768)
                     /locus_tag="IU325_22455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          939 a          681 c          654 g          884 t
ORIGIN      
        1 taaaatcaga taaacatttg aggtattgtt atgacaaata aacacccatc attgttttct
       61 ccttttatgc tgacagaaaa aataaagcta cgtaatcgta tcgttatggc tcctatgaca
      121 acctggtcag ctaatcctga tggaaccatc tctgagcaag aactcgaatt ttataaacgt
      181 cgctcacaaa atgttggttt agtaataaca ggatgcacct atgtcacgcc aagcggcatt
      241 gggtttactc atgaatttgc cgcgtatgac gaccgtttca ttaacagcct tgaaaagtta
      301 gcagctgcag ccaaaagtgg cggtgctccg gctattttgc agattttcca cgctggtaat
      361 aaagccatcc cggagcttgt gccaaataac gatgttatta gtgccagcgc atcaagcgta
      421 aaatctggtg actttatgaa gagagtggta caaagcaggg aaatgactga aaacgaaatt
      481 caggaaacca ttagagcatt cggagatgtc acaaaaaggg cgattaaagc aggttttgat
      541 ggcgttgaac ttcatggtgc gcacggattc cttctacaga atttcttctc ccctctattt
      601 aaccagcgta atgaccgatg gggaggagat ctcgaaagac gtatgcgatt cccgctggcc
      661 gtattacagg aagtaaaaaa tgtagtctat gaatatgcga caaagccctt tgccatcggt
      721 tacagaatat ctcctgaaga atctgcaaca ggggggctgc ggatagagga tacttataaa
      781 ctccttgatc ggctcattgc atccggtatt tcgtatatac atacctcgct ggtcagtatt
      841 aacgatagct accctgttga gtcgccaaat ggcccccgaa ctattgaatt gattttaaat
      901 catattgcag gcagagtgcc ggtaatcgct gcaggtaaaa tacgcacacc gtctcaggct
      961 caagaagcaa tttccactgg cctgccactt gttgcgatcg gtaaagggct ggtgataaat
     1021 ccagaatggg tgactttagc tgaatcaggt agaagccatg aaattcaaac aacccttaat
     1081 ccacaacttg ttcctgaatt aaccattccg gataaactat gggatcaaat tcaggcatcg
     1141 aaaggaactg gctggtttcc gttaatggat taaatatcta ttactctccc gagtgtaaaa
     1201 ctatcaaacc aatgagtaac cgatacagcc tcgcaaattc tttcagataa ttaacccgtc
     1261 actgcggcac gttctctcat aaggcgtgcc tgctgtgtaa tccagtatgg atccagcata
     1321 gttgacggct taatagactc aattcctttc ccaggttacc aacaacgata tcttgtataa
     1381 caccccagaa gatacgacat tatgatgaga tgaggtatgc tcctcagcgc caatcaacgc
     1441 cagggctggg ccagttttga gatcagactc attcccggac tgacttcagg cgacatatat
     1501 caatgacgtt ggggcaataa aagatagccg atgtaataca tctattcaaa tttgtgatac
     1561 atgtcaaaaa attggttgac caaactcgtt atttatataa gggcacttac gaagtgcact
     1621 cttttttaaa gcgaggaagt accaatgaaa gagaataaag tacaacaaat cagtcataaa
     1681 ctgattaata tcgttgtttt tgtcgcaatt gtagaatacg cctatttatt tctccatttc
     1741 tattaataac ggcaataaac tgttcacttc agtgatatta aaaaatatgc atcctctccc
     1801 ttttttgtaa gtaattatta tatccgtggg agaggaatac acgctgtccg gcaatcaatc
     1861 aaactgcgat aaatcatcgg ccagtaaaat ggagatagcc tccattctcg aaaaattcat
     1921 actctcagcg aaaccatcac tcatcaaggc gtttatggga gcgtcgccaa tggctgctaa
     1981 caatgccaga cttccccgtt gccgaaattc cacataccac aaatggtcac agggattggg
     2041 tgttgaaatg atccggatga gcatgtatct ttatggttat gttataacat aacaggtaaa
     2101 aatgatgaag cccaatatcc atcctgagta tcgtactgtg gtgttccacg acaccagtgt
     2161 tgatgagtac tttaaaatcg gctcgactat caaaacagac cgtgagattg agctggatgg
     2221 cgtaacgtat ccgtacgtga caattgatgt ctcttctaaa tcgcacccgt tctatacagg
     2281 gaagctgaga acagtggcat cagaaggaaa tgttgcacga ttcacccaac gttttggtcg
     2341 ttttgttagc acgaaaaagg gggcatgatg aaagtcctta actctctgcg taccgcaaaa
     2401 gaacgccatc cagactgtca gattgtgaag cgaaaaggac ggctatatgt gatttgtaaa
     2461 tctaatccac gttttaaggc cgttcagggt cgtaagaaaa aacgttgatt caaaattcga
     2521 cgaattaacg atatttgtct gattaataat cagaccggat taatgttggt gtatttatta
     2581 caccagcatt aattttcctg gggatgtatc ttcctgttca tttaaagtca actgcctgac
     2641 gtttctctcc gaatattcca ttatattaat gttgacttgt tgaccaggtg cgccctgtat
     2701 gctggcatca actctctttt agactgaaca cgccactcag tttcctccct ttgcgacgca
     2761 gcctgcattt acactcaaac ggttaagatg ataaatgtgg taaatctgtt ggtactgaca
     2821 taaaaacgtt tacgccacag gaacggcctg atccaccggt aaccccatcg ccgacgttcg
     2881 agtgccagtt agagtaacgc gcacagatga ctgaatgcag tgccctggca aagaggccat
     2941 cgttcctgtg acaactggca gtcttcgttt aacttcactt aatttggctc ttagggggct
     3001 taccggacag atgacgtact tacacctgtt taatttttca tcacttattg ggatgaaccc
     3061 ccataaccat tttgtgcggc atgttaatcc attaaaacac cttactgatt ggcaaatcat
     3121 ctttaattat gactcatgat agttttatat tctatttc
//
LOCUS       DACBVI010000268         3121 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.272, whole genome shotgun sequence.
ACCESSION   DACBVI010000268 DACBVI010000000
VERSION     DACBVI010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.272"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..1015
                     /locus_tag="IU325_22460"
     CDS             <1..1015
                     /locus_tag="IU325_22460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscV/YscV/HrcV family type III secretion system
                     export apparatus protein"
                     /protein_id="HAP5811741.1"
                     /translation="NKLDQVITETVPLVLIMNSVQAKKYTEINLADRIRSQFFIEYGI
                     RIPGIVIREGEGLNDEDVILMLNEVRASQFKIYHDLVLLVEYSDEVVSTLIKKPVIVN
                     SNGEQYYWVTKSDAQKLTKIGCYTRTAMDEMYNHLSVCLAHNINEYFGIQETKYILDQ
                     LEMKYPDLLKEILRYITVQRISEVIQRLIQERISVRNMRLVMEALALWSPREKDIITL
                     VEHVRGALGRYICHKFSYSGEIKAIVISPEIEDRIRDGVRPTAGGTFLNLDASEAEMI
                     LDNFKLALSGINIPIKDIILLGSVDIRRFIKKLIESSYRDLEVLSYGELTENVPVNIL
                     KTI"
     gene            1047..2339
                     /locus_tag="IU325_22465"
     CDS             1047..2339
                     /locus_tag="IU325_22465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HAP5811742.1"
                     /translation="MKKLKLLNKYSYLHSINGSLIEAELDDVSVGEVCEIYASWQANE
                     RIARAQVVGFRNGKTLLNLIGSSVGLTRTAVLKPTGEQLTIQISDAFLGSVLNASGQI
                     MERFVPDTPGERGNLRLIDELPPSYQERRVINTPLETRIRVIDGVLTCGIGQRVGIFA
                     SAGCGKTVLMHMLVNNTEADVFVIGLIGERGREVTECAESLKKSVNAAKCVLVYATSD
                     FSSVDRCNAALMATTVAEYFRDRGKRVVLFIDSMTRYARALRDMKLAAGEPPARRGYP
                     ASVFDSLPRLLERPGPTLKGSITAFYTVLLEGEDESDPLGDEIRSILDGHIYLSRKLA
                     GQGHYPAIDVLKSVSRVFGQVTDEKHRDNAARVRKILTTLEDLQVFIDLGEYRAGQNA
                     ENDFAMNARPKLTNWLKQSVNEKMPMSETLKELERIVK"
     gene            2329..2787
                     /locus_tag="IU325_22470"
     CDS             2329..2787
                     /locus_tag="IU325_22470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HAP5811743.1"
                     /translation="MLSKVNRLIRRTAQSLAACEASLQKLNAEKEKLAEKERLYDMQL
                     KNLQSLLDVKELLGEVVFRQDIFYSLRKVAVIQQQIAEINLEKQKIAERRKILNKEIV
                     QQQAQRKHWWLKGEKYDRLKKRIKKQLLNQMLYQDELEQEEKYNGRSQEN"
     gene            2765..2995
                     /locus_tag="IU325_22475"
                     /pseudo
     CDS             2765..2995
                     /locus_tag="IU325_22475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311755.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
BASE COUNT         1021 a          535 c          707 g          858 t
ORIGIN      
        1 taataaactt gatcaggtta ttaccgaaac ggtacccttg gttttgatta tgaatagtgt
       61 acaggctaaa aaatacactg aaatcaatct tgctgatagg attcgtagtc agttttttat
      121 tgagtatggt atccgtattc ccggtattgt cattcgcgag ggggaaggcc ttaatgatga
      181 agatgtcata ttaatgctaa atgaagtcag agcctcgcaa tttaaaattt atcatgatct
      241 cgtcttgttg gttgaatatt cagatgaagt ggtttcgact ttgatcaaga aacctgtcat
      301 cgtaaatagt aatggtgaac aatattattg ggttacaaaa agtgatgccc aaaaattaac
      361 aaaaattggc tgttatactc gcacggccat ggatgaaatg tacaatcatt tatctgtatg
      421 tctggcgcat aatattaatg agtattttgg tatacaagaa accaaatata ttcttgatca
      481 attagagatg aagtatcctg atctattaaa agaaatatta agatatataa ccgtgcagcg
      541 catttcagag gtaattcagc gtctgatcca ggagcgaatt tcagtgcgca atatgcgttt
      601 agttatggag gctttagcat tatggagtcc acgggagaaa gatataatta cactcgtcga
      661 gcatgtacga ggtgcattag gtcgctacat ttgccataag ttttcatatt ctggagaaat
      721 taaagcaatt gtgatttcgc ctgaaataga ggacagaatc agagatggtg tcaggcctac
      781 cgctggcggt acttttctta atctcgatgc atccgaagct gagatgattc tggataattt
      841 taaactggca ctttctggaa tcaatattcc tattaaagac attattttac tgggatctgt
      901 ggatattcgt cgttttatca aaaaacttat tgaatctagt tatcgagacc tcgaagtact
      961 ttcgtacggg gaactaacgg aaaatgttcc cgttaatatt ctgaaaacta tttaggataa
     1021 tgtcttgcga tatggagcat gctggtatga aaaaattaaa actattaaat aaatacagct
     1081 acttacatag cataaacggc tcgctgattg aagcagagct tgacgacgtt tcagtgggcg
     1141 aagtatgcga aatttatgcg agctggcaag caaatgaacg tatagccagg gctcaggtcg
     1201 ttggctttcg caatggaaag acattattaa accttatagg cagctctgtg gggcttacac
     1261 gtactgcagt tttgaaacca acgggagagc agttaaccat acagatcagt gatgcctttc
     1321 ttggttcagt tttgaatgct tctggtcaaa ttatggaaag gtttgtcccg gacactccag
     1381 gcgaaagggg aaatttacgc ctgattgatg aactgccacc gtcgtatcag gaacgtcgag
     1441 ttattaatac accactagag acacggataa gagtcattga tggcgtactg acatgtggca
     1501 ttggtcaacg tgttgggatt ttcgcatcgg ctggctgtgg caaaacggtt ttaatgcata
     1561 tgcttgtaaa caacactgaa gctgatgtgt ttgttattgg cttgattggc gaacgtggta
     1621 gggaagttac ggaatgcgcg gaatcgctga aaaaatcagt aaatgcagca aaatgtgttt
     1681 tggtttatgc cacctctgat ttttcgtcag tagatcggtg caatgccgcc ctgatggcga
     1741 caaccgtggc agagtatttt agagatcgag gaaagagagt tgtacttttt atcgactcaa
     1801 tgacgcgata tgcacgagca ctacgcgata tgaagctggc agcgggtgaa ccacctgcca
     1861 gacgtggtta tcctgcttct gtttttgaca gtttacctcg cttactggag aggccgggac
     1921 caacactaaa aggaagtatt actgcgtttt ataccgtatt actggagggg gaggatgaat
     1981 cagacccact gggtgatgaa attcgctcaa tccttgatgg ccatatctat ctaagtcgca
     2041 aactcgctgg gcaaggtcat tatccggcaa tagatgtact aaaaagtgtg agtcgagtat
     2101 ttggtcaggt cactgatgaa aaacacagag ataatgctgc gcgggtaagg aagatcctca
     2161 caactttgga agatctacag gtgtttattg atttagggga atatcgcgct gggcaaaatg
     2221 ctgaaaatga ttttgctatg aatgcacggc ctaaactcac aaattggttg aaacagtcag
     2281 ttaatgaaaa aatgccaatg agtgaaacgc taaaagagct tgaacgaatt gttaagtaaa
     2341 gttaatcgac ttatcagaag aacagcgcag agcctggctg catgtgaagc atcattacaa
     2401 aagttaaatg cagaaaaaga aaagttagcc gaaaaggaac gcctgtatga tatgcaactt
     2461 aaaaacctac agtcactgtt agatgtgaaa gagttactcg gcgaagttgt atttcgacag
     2521 gatatatttt actcgttacg taaagttgca gtcattcagc agcagatcgc tgagataaat
     2581 cttgaaaagc aaaaaatagc agagcggcga aaaatactta acaaggaaat agttcaacag
     2641 caagcacaaa gaaaacactg gtggctaaaa ggagaaaagt acgatcgact aaagaagcgc
     2701 atcaaaaaac aacttttaaa tcagatgctt tatcaagacg agcttgaaca ggaggagaaa
     2761 tataatggac gaagtcaaga aaattgaaca ctgtaaaatt ttaaacacag acacacccga
     2821 taattctgct tcagatttag acagtttctt aaagaaaaac aaaaaaaata aagaaatgag
     2881 tgagattgtc gctggaacca ttctccagtt attacgtcag actcaggata gccaattttc
     2941 tacgttgggg aaaaaaaatg ggcacatgga gtctattgac aacaaatcat ctctccaggt
     3001 tgacaggaat ccatctcttt ctttgcaaac aaatggaaca gagaaatttt ctacaaagaa
     3061 aattctcaat atgacttcac gagatcaggg gaatgattct gaaccatcta ataaaacgat
     3121 a
//
LOCUS       DACBVI010000269         3017 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.273, whole genome shotgun sequence.
ACCESSION   DACBVI010000269 DACBVI010000000
VERSION     DACBVI010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.273"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            119..970
                     /locus_tag="IU325_22480"
     CDS             119..970
                     /locus_tag="IU325_22480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP5811744.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINTGYRLID
                     TAASYQNETQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNKVVPAVNQVEV
                     NPFNQQLQAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWI
                     YQRGIASLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
     gene            997..1986
                     /locus_tag="IU325_22485"
     CDS             997..1986
                     /locus_tag="IU325_22485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP5811745.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGDDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGAIKPG
                     TTFGKDDYRSTVPRFATQAIEANEKLVTLLGELAAEKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLNRLEENLAAADIVLSQKDTQQITEALETIKIVGERYSPEHQARVGR"
     gene            complement(2017..2910)
                     /locus_tag="IU325_22490"
     CDS             complement(2017..2910)
                     /locus_tag="IU325_22490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP5811746.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAMKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQQNGTPSGNIRLSAGE
                     HAARTLVWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPQTPHELQNHRCINMRLPTAGGLYHWEFEREGKPLRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIKSGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
BASE COUNT          790 a          779 c          731 g          717 t
ORIGIN      
        1 tcgcaacact caaacagatt tatgaatttc gttcataacc cctgacaaat cctgccgtct
       61 aatcgagctc ttctctttac gttatgcttc tcatgaacga taacggaagg ataacaccat
      121 gcaaaaagta aaactgaaca acggtattga aatgcccctg ctgggctttg gtgtatttca
      181 gatgaccgat gccgctgaat gcgaaagagc ggtcattgac gctattaaca ccggttatcg
      241 actgattgac accgccgcct cctatcaaaa tgaaacccag gtcgggaacg cactgaaaca
      301 aagcggtatc gcccgtaacg aactctttgt caccacgaaa ctctggttgc aggataccag
      361 ttacgaaggg gccaaagccc agttcgaacg ctctctgaat cgactgcaac ttgattacgt
      421 tgacctgtat ttgattcacc agccatacgg cgatgtccac ggagcctggc gtgccatgga
      481 agagctgcaa caggccggta aaattcgcgc tattggcgtc agtaacttcc atcctgaccg
      541 actggctgac cttatcgcct ttaataaagt cgttccggcg gtaaaccagg ttgaagttaa
      601 ccctttcaat cagcagctac aggccgttcc ctggatgcaa agtcgtggca ttcagccaga
      661 agcctgggca ccgtttgcgg aagggaaaaa tggtctgttc cagcatcctg ttctcacggc
      721 aattggcgag aagtatggca aaagcgtggg ccaggttgtt ctgcgctgga tctaccaacg
      781 aggcattgct tcactggcaa aatcagtgcg aaaagaacgc atggaagaga acatcaacat
      841 tctcgatttt gaactcagcc ctgaagatat gctgcaaatt accgccctgg ataccgcaac
      901 cagcgcgttc ttctctcacc gcgatcccgc aatggtggaa tggctgactg gccgcaaact
      961 tgatgtttaa gccgcgataa aagaggtatt cattcaatgc aaaaacgtta tctgggtaaa
     1021 tccggactcg aagtctccgc ccttgggctc ggttgcatgg gcttaagcca cggctacggc
     1081 ccggcgaccg atacccgtca ggctatcgaa cttattcgtg ctgcggttga acgtggtgtc
     1141 accttcttcg ataccgccga agtgtatggt ccgtatctga atgaagaagt ggtcggcgaa
     1201 gccttaaaac cgtttcgtga ccgcgtggta attgccacta aattcggttt taccttcggc
     1261 gatgataata agcagcagat tttaaacagc cgtccggaac atatccgtga agccgttgaa
     1321 ggatcattac gtcgtctgaa aacagatgtt attgacctgc tgtatcaaca ccgtgttgat
     1381 ccggatgttc ctattgaaga tgttgcagga acggtgaaag acctcatcgc agaaggtaag
     1441 gtcaaacatt tcggcctgtc tgaagcagga gcacaaacca ttcgtcgtgc acatgcggta
     1501 caacctgtca ccgccctgca aagcgaatat tccatgtggt ggcgtgagcc tgaacaggaa
     1561 attctgccgt tgctggagga actgggaatt ggttttgtcc ccttcagccc actaggtaaa
     1621 ggtttcctga caggggcgat taagccggga accactttcg gcaaagatga ttaccgcagc
     1681 accgtaccgc gtttcgcaac acaagccatt gaagccaatg aaaaactggt caccttgtta
     1741 ggtgaactgg ctgcggagaa aggggtaacg tctgcacaaa tcgcactggc atggctatta
     1801 gcacaaaagc cgtggattgt tcccatccct ggcaccacta aactaaaccg gctggaagaa
     1861 aacctggccg ctgctgacat tgttctttcg cagaaagata cgcagcagat aaccgaggcg
     1921 ctggaaacaa ttaaaatcgt cggtgaacgt tactcgcctg aacatcaggc acgcgtaggc
     1981 cgctaatacc caaacgggtc caggataaag gacccgttat tcggtgtagc gaagcgcatc
     2041 gatcaacagc gcaaaagctg gcggatgctg cttgcggctg gggtagtaaa gataataccc
     2101 aggaaaagtt ggacaccact cctgtaacac ctgaataagc ttgcctgact ttatatactc
     2161 ctgaaccatg tcttcaggga cacaagcgat cccaaaccct gataaggcgg catcaattct
     2221 ttctggcaac ggactacacg tcagctgccc atcaacctta acgcgaagcg gtttcccttc
     2281 tcgctcgaac tcccagtggt aaagcccacc ggcagttggc aaacgcatgt tgatgcagcg
     2341 atgattttgt agctcatgag gcgtttgagg cgcagggttt gcagcaaaat acgccggagc
     2401 tcccaccaca gccatccgca tatctggtcc aatcctgact gcaaccatat ctttatcaac
     2461 attttcaccc agtcgtatcc cggcatcaaa acggccttca acaatatcga caaaaccgtt
     2521 atccaccacc agttcgacat tgatttcggg atattctctg agaaaaggtt ttagcttcgg
     2581 ccagaccaga gttcgcgcgg catgttcccc ggcagataac cgaatattcc cagagggcgt
     2641 gccgttctgt tgaataagtg actccagttc ctgttcaagc tcgtcaatac gcggttcaag
     2701 gcaggcaatg attctctccc ccgcttctgt gggagccaca ctgcgggtag tccgtgtcag
     2761 gaggcggata ttgagccttt cctccagagc cttcattgca tggctaagtg cagactgaga
     2821 gacgccgagt ttgcccgctg ctttggtaaa acttcgctct cttgccacca caagaaagat
     2881 ttgcagttcg ttgaagtttt ctttgagcat tggcgatttc cttgtagaaa catcctcttc
     2941 acagacactc atgaaggggg gcatagatgc tattatttta accacattat tagtaacaat
     3001 gataatctgg ctgtatc
//
LOCUS       DACBVI010000270         2986 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.274, whole genome shotgun sequence.
ACCESSION   DACBVI010000270 DACBVI010000000
VERSION     DACBVI010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.274"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            240..965
                     /locus_tag="IU325_22495"
     CDS             240..965
                     /locus_tag="IU325_22495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAP5811747.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            923..1600
                     /gene="bglJ"
                     /locus_tag="IU325_22500"
     CDS             923..1600
                     /gene="bglJ"
                     /locus_tag="IU325_22500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HAP5811748.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLTNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1638..2426)
                     /gene="fhuF"
                     /locus_tag="IU325_22505"
     CDS             complement(1638..2426)
                     /gene="fhuF"
                     /locus_tag="IU325_22505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HAP5811749.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRAACFWVDVCEDKNATPHSPQQRMERL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2567..2803
                     /locus_tag="IU325_22510"
     CDS             2567..2803
                     /locus_tag="IU325_22510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 family protein"
                     /protein_id="HAP5811750.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTS
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            complement(2842..2928)
                     /locus_tag="IU325_22515"
     tRNA            complement(2842..2928)
                     /locus_tag="IU325_22515"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2892..2894),aa:Leu,seq:cag)
BASE COUNT          790 a          647 c          742 g          807 t
ORIGIN      
        1 tattcctaac aacttaatgt agatatttta aatgtctcca ggctatttca ccaggttatc
       61 atttgatgct aaccttctga cacatgcagt ggagttgttg tgcagcagga gtatgctgat
      121 atgaaagtga atgctaagga taatttattc gcttaatcta ttaatttgct ggaaacattt
      181 aaggaatgaa tatcctgaat aaaccatcat aatttttaga cagtggatgt ggaggaaata
      241 tgttgccagg atgctgcaaa aatggaattg ttatcagtaa aatacctgtt atgcaagcag
      301 ggctaaaaga ggtcatgagg actcacttcc ctgaatatga aataatatcc agcgcctctg
      361 cggaggacct taccttatta caattacgtc gttccggatt agtcattgct gatttagccg
      421 gtgaaagtga agatccacgt tctgtttgtg aacattatta ttctttaatc tcacaatatc
      481 gggaaattca ctgggttttc atggtgtcac gctcctggta ttcccaggca gtagaattgc
      541 tcatgtgccc tacggcgacg ttattgtctg atgttgaacc cattgagaat ctggtcaaga
      601 ccgtacgttc cggcaatacg cacgcagagc gtatcagcgc catgctgacc tctccggcaa
      661 tgaatgaaac tcatgatttt agctatcgct ccgtcattct cactctttca gagcgcaagg
      721 tactgcggct attaggtaaa ggatggggca tcaaccagat agcttcattg cttaagaaaa
      781 gtaataaaac tatcagcgcc caaaaaaaca gtgcgatgcg tcgactggca attcacagca
      841 acgctgaaat gtatgcatgg ataaatagcg cgcagggtgc aagagaactt aacttgcctt
      901 ctgtttatgg agatgccgca gaatggaaca cagccgaatt aagaagagaa atgtcgcact
      961 catagaaaaa tgcgtcatga gcagtatcgg tattgagagt ttattcagaa agtttgcggg
     1021 taacccttat aagctccata cctataccag tcaggagtca tttcaggatg ccatgtcgcg
     1081 gatctcattt gcggcggtca ttttttcttt ttctgccatg agaagtgagc gcagagaggg
     1141 attatcttgc ctgactgaac tggcgattaa gtttccgcgt acccggcgtt tagttattgc
     1201 ggatgatgat attgaagctc ggctgattgg ttcattgtcg ccatcaccgc tggacggtgt
     1261 attaagtaaa gcgtcaacgc tggagatttt tcatcaggaa ctctttttgt cattaaatgg
     1321 tgtacgtcag gcgaccgacc gactgaccaa tcagtggtac attaaccaaa gccggacgtt
     1381 aagcccgacg gagagagaaa tattgcgctt tatgtcgcgt ggctactcaa tgacacaaat
     1441 tgccgagcag cttaaacgca atatcaaaac gatccgtgca cataaattta atgtgatgtc
     1501 gaaactgggc gtcagttctg atgcagggtt gttggaggcc gcagatattc tgttatgtat
     1561 gcggcattgc gaagcaagta atgtgttgca tccctattaa tccgcattat gccgggttta
     1621 cttccccggc agtgctttca tttcagcgta caatcgccac attgctgcac atccggtaag
     1681 cgataacgct ggcagcaagt gcggcgcacc agcaggccat cgcgcagtac cacggtacgc
     1741 cagagtggat tatcttcacc gttcgtgagc gttttctcaa aaaagagggc atggcgcagc
     1801 gattcaaccg tcgcctcgcc gagcagttgc ttcatctcag taagatacca gttgatcaaa
     1861 taaccggtat tactccagat aagtttgccg ttgatctctc cggtcgcttc tagtgcctgc
     1921 acaaccggaa ccagcgcctg gctgattaac ctttccattc gctgctgcgg cgaatgtggt
     1981 gttgcgtttt tatcttcaca cacatcgacc cagaaacagg cggcacggcc ggtttcgtga
     2041 aactcagcat ggaaatgttc cggcgacaca tctaatgcct tttcctgcgt cagtagcgcc
     2101 agcattaatg gtggcaccat caggccgata taccattgtg cccatagcga gatcagcggt
     2161 ttgttctcgc ggatcatcgt tggttggttg cgatagatat gatcggaata gaccgcgagt
     2221 agagaactta gcgcattcgg tgatgaccat tgcgccagcg tcatggcgtt aagcggagcg
     2281 ggttcatcca ggcggataaa ctccagcaaa tgttcacgat gtttggcgat cgtcgcacgt
     2341 acggcttgtg caagcccagc atcctgtggc tggagatgcg ttcgccagat aacatcttca
     2401 tagagcggtg cggaacgata ggccataatc gggatagtaa tctaaatgat aatgattgct
     2461 aatcctagcg ataggtttat ccgatagcaa gggatttctc tggcttgcaa atgataaaaa
     2521 ttatcatatg atattggtta tcattatcaa tgaaagagat gaaaccatgt tgcaacgtac
     2581 gctgggcagt ggctggggag tgttgctgcc aggattgctg attgcagggc tgatgtatgc
     2641 ggatttatca ccggatcagt ggcggattgt cattctgatg ggattagtat tgacgtcggt
     2701 gatgctgtat cacaaacagt tacggcatta cgttttgcta ccatcgtgcc tggcgcttat
     2761 tgctgccatc atgctgatga taatgaattt gaatcaggga tgaaaaatca aggaagaaac
     2821 aagaaaggaa gtaaagataa ttggtgcgag gggggggact tgaaccccca cgtccgtaag
     2881 gacactaaca cctgaagcta gcgcgtctac caattccgcc accttcgcac agtcatctta
     2941 cttttttgat atcgcctcgt ttggtgcgag gggggggact tgaacc
//
LOCUS       DACBVI010000271         2914 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.275, whole genome shotgun sequence.
ACCESSION   DACBVI010000271 DACBVI010000000
VERSION     DACBVI010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.275"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            340..>2914
                     /locus_tag="IU325_22520"
     CDS             340..>2914
                     /locus_tag="IU325_22520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811751.1"
                     /translation="MLVKTPVNPLLQWLNMFFSRRSLSGADGRALYAYRCTDTEYESL
                     AELLRTYAPRSYPRTIFISYSDVLFSIYAAEFIRRTHTVGHPKWDTILDSIDWKVPYV
                     HRQKLVNDGIRYWKRKIRNLGQASGYLHTLACEGGLPIRMIENESGYLITYFRRIYQA
                     LRGQASQYPAAKIAQELGDTIPVTMQNELVYEIAGEFCETLCRLLSEHPPQSSDPVSA
                     LRKLSPDWHLQLPLVLPEANAAEIVRRLLSQSSEIRSASSLQVERIWVDVDDSWYCDA
                     RFRFPATMRTEQLTSLFECNIQPEQTRLIISGKWRNGGARLAMLSRYEQQDWRVELLP
                     IAMQKLSGADAMAEISLSLHEGPILLGHTIPKGGYELTEELPWVFEAMNESESQLKLV
                     GMGSVSSRLNALFISLPKNSHLDISGEGEFDIPRLLKNSERSLTKISGVFSVVLHDGA
                     VCTIRTQQLYDSAIEYYIKSTEVELVKSDYPVHRAWPKIGWKKDLQYGIVPEKELFWR
                     SIRSGNNAWHSVASEMPKGQIEVRRIVNDEVLFSGKVVVLPADFDINIIPESVQQGII
                     MLSGITDTRIDKYSNNEKVTLKSDYSQNECAIYYNSSEMLENTVDLRVSWKDGSNLKL
                     LLPKPVSGGRFVTHDGSVHFDGVASIAHLHGIDAELLTISCAGRGYLNIELLDENPVA
                     EKFRYLHADLPLLSGCNDKLKQISLYENYNLLNAMLACAWNSNSTLCVDFYSDRFGKD
                     KATLSIKRYDGSFIEHDQGLLVDIKNSVVFPANRIDELVVDAISLKDPGLHISLLKKD
                     EFAYDLSALNVQDSPWLIVGKLDGTARIAPVIKWGLPVLQTNDLLLNALCEADSEQRK
                     KY"
BASE COUNT          861 a          526 c          642 g          885 t
ORIGIN      
        1 ctatgtatat tttttttgtc acaccaccac gctgataaaa atacgaatat aacagcccag
       61 atgaaaatat tggtgatcag tgtagagtct atcgattgaa acaactgctc aaaaagctgc
      121 gctaacattt tgatacctgt gtaaataatg gatagttgta attgtttaaa aaataaacaa
      181 aacacacata atttatatgg ttaagttcgg tgtagcgctc atgattgtaa caagtttttt
      241 cgagatataa atgatttgtc tcatttttag cgagttcatc gggttgagct tttttatgat
      301 gatagtaacc tgttacgaat tgtatcaaag gaaggcaggt tgcttgtgaa aactccagtt
      361 aacccacttt tgcaatggct taacatgttt tttagtcgcc gttcattatc cggagctgat
      421 ggacgggcgt tatatgctta ccgttgtact gatacagagt acgaaagttt ggcagaacta
      481 ctgcgtacat atgccccccg tagttatcca agaacgatat tcatttccta tagcgatgtt
      541 ctatttagca tatatgccgc agagtttatc cgccggactc atacggtcgg acaccctaaa
      601 tgggacacaa ttttagattc tattgactgg aaagttccgt atgttcatcg acagaagctg
      661 gttaatgatg gcattcgcta ctggaaaaga aagataagaa acctggggca agcttcgggt
      721 tatctgcata cactggcttg tgaaggtggt ttacctatcc gcatgattga aaatgagagc
      781 ggttatttga ttacctattt cagaaggata tatcaggcgc ttcgtgggca agcatctcaa
      841 tatcctgccg ctaaaattgc acaagaatta ggcgatacga ttcctgtcac aatgcaaaac
      901 gaactggtct atgaaatagc cggtgaattc tgcgagactc tctgccgatt acttagtgag
      961 catccacctc aaagcagtga tcccgtttct gcattacgta agctctctcc agactggcat
     1021 cttcaacttc cactcgttct ccccgaagcg aatgctgcag agattgtaag gcgattactt
     1081 tctcaatctt ctgaaatacg cagtgcaagt agtcttcagg ttgaaagaat ctgggtcgat
     1141 gttgatgaca gttggtattg tgatgcccgg ttccgattcc cggccacaat gcgtacagaa
     1201 cagttaacct ctttgtttga atgcaatatt cagccggagc agacccgact catcatttca
     1261 ggaaaatgga gaaatggcgg ggcaaggttg gcaatgctaa gccgttatga gcagcaagat
     1321 tggcgggtcg agttattacc tattgcgatg caaaagctct ctggtgcaga tgcaatggcc
     1381 gaaatctcgt tgtcgcttca tgaaggtcca attctgttag gccacacaat tccaaaaggc
     1441 ggttatgaac ttactgaaga actcccctgg gtttttgaag cgatgaatga gagtgaatcg
     1501 cagctaaaac ttgtcggtat ggggtctgtg agttccaggc taaatgctct gtttatttcg
     1561 ctaccgaaaa atagccattt agatattagt ggagaaggtg agtttgatat cccaaggttg
     1621 ctgaaaaaca gtgaacgcag cttaactaaa ataagtggag tatttagcgt tgttttacat
     1681 gatggtgcag tttgtacaat tcgcacccag caactttatg attctgcgat tgaatattat
     1741 attaaatcga cagaagttga actggttaaa tcggattatc ctgtccatcg agcctggccg
     1801 aagatcggtt ggaaaaagga tctgcaatat ggcattgtac cggagaaaga acttttttgg
     1861 cgttccattc gttcaggtaa caatgcctgg cattctgtcg catcagaaat gccaaaagga
     1921 cagatagaag tcagacgaat agttaatgat gaggttttat ttagcggtaa agttgtagtt
     1981 ttaccagcag acttcgatat taatattata cctgagagtg ttcagcaagg catcataatg
     2041 ctttctggta taacggatac tagaattgac aaatattcaa acaatgaaaa agtaacactt
     2101 aagtccgatt attcacaaaa cgagtgtgct atttattata attcatcgga gatgctggaa
     2161 aataccgttg atttacgggt ctcctggaaa gatggttcta atcttaaact attattacct
     2221 aagccggtta gcggtggccg atttgtaact catgatggtt ctgttcattt tgatggtgtg
     2281 gcatctatag cgcatttgca tggaatagat gctgagttgt taaccatatc ttgtgctgga
     2341 agaggatatc ttaatatcga gttgttggat gaaaatccag tagctgaaaa attccgttat
     2401 ttacatgccg accttcctct tttatcagga tgcaatgaca aattaaaaca aatttcactt
     2461 tatgagaact ataatttgct aaatgctatg ctggcatgtg catggaacag caatagtaca
     2521 ttatgtgttg atttttactc tgacagattt ggaaaggata aagcaacact cagtattaaa
     2581 cgctacgatg gtagttttat tgaacacgat caagggttac tggttgatat aaaaaattct
     2641 gttgtttttc ctgcaaatag aatagacgag ttggttgttg atgctatttc tcttaaggat
     2701 cctggcttac acatatcgtt gctaaaaaaa gatgaatttg cttatgatct ttcagctctg
     2761 aatgttcagg atagcccatg gttaattgtg gggaaacttg atggtacagc tcgcattgca
     2821 ccagtaatta aatgggggct acctgtatta cagacaaatg atttattact taatgctcta
     2881 tgcgaagcgg attccgaaca gcgaaaaaaa tatt
//
LOCUS       DACBVI010000272         2881 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.276, whole genome shotgun sequence.
ACCESSION   DACBVI010000272 DACBVI010000000
VERSION     DACBVI010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.276"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            159..2438
                     /gene="ydeP"
                     /locus_tag="IU325_22525"
     CDS             159..2438
                     /gene="ydeP"
                     /locus_tag="IU325_22525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HAP5811752.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGERLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLTVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2686..>2881
                     /gene="safA"
                     /locus_tag="IU325_22530"
     CDS             2686..>2881
                     /gene="safA"
                     /locus_tag="IU325_22530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HAP5811753.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
BASE COUNT          773 a          668 c          695 g          745 t
ORIGIN      
        1 ttatcgtatt taatcttttg tttaacgata atagattaaa atagaaagag agtagtcccc
       61 tactcttata tatccatgtt gacgataatc cctttgtata tactgtgcat catcgctatt
      121 acaaatccta ataattcatt tccacacagg ataagcagat gaagaaaaaa attgaatcct
      181 accagggagc tgccggtggt tggggtgctg ttaaatccgt agcgaatgca gtacgtaagc
      241 agatggatat acgccaggat gttattgcca tgtttgacat gaataagcca gagggctttg
      301 actgtccggg ttgtgcatgg ccagatccta agcacagtgc gtcattcgac atttgtgaaa
      361 acggcgcaaa agcaatcgcc tgggaagtca cggataagca ggtaaacgcc tctttctttg
      421 ctgagaatac ggttcaatca ttacttacct ggggagacca cgagcttgag gctgcggggc
      481 gtctcaccca gcctttgaaa tatgatgccg tcagcgactg ttacaagcca ttaagctggc
      541 aacaagcttt cgacgaaatt ggcgaacgcc ttcaaagcta tagtgatccc aatcaggttg
      601 aattctatac ttcgggccgc acttccaatg aagctgcctt tctttatcag ctttttgccc
      661 gtgaatacgg gagcaataac ttccccgact gctccaacat gtgccatgaa ccgaccagtg
      721 tgggtttggc agcgagtatc ggtgtaggta aagggaccgt gttgctggaa gactttgaga
      781 aatgcgattt agtcatttgc attgggcata accctggtac aaaccaccct cgcatgttga
      841 cttcgttgcg cgctttagtg aaacggggag cgaaaatgat cgccatcaat cctctacagg
      901 aacgtggcct ggagcgattt accgcaccgc aaaacccgtt tgaaatgctg acgaactctg
      961 agactcagtt ggccagtgcc tactataacg tgcgcattgg tggtgatatg gcgttgctca
     1021 aggggatgat gcgcctgtta attgagcgcg atgatgctgc aagcgccgca ggtcggccct
     1081 cattgctgga tgacgaattt attcaaacgc ataccgtcgg ctttgacgag ctacgccgtg
     1141 acgtgctcaa ttccgagtgg aaagatatcg aacgtatttc tggattaagt cagactcaaa
     1201 tcgccgaact ggctgatgca tatgccgctg ccgaacgcac cattatctgt tacggaatgg
     1261 ggatcactca gcacgaacat ggtacccaga atgtacagca actggtcaat ctgctgttga
     1321 tgaaaggtaa cattggcaag cctggtgcgg gtatctgccc actacgaggt cactctaatg
     1381 tacagggcga ccgaaccgtc ggtatcaccg agaaaccgtc tgcagagttt ctggctcgcc
     1441 tgggtgagcg ctatggtttc accccacctc atgcacctgg acatgctgca attgccagca
     1501 tgcaagcaat atgtacgggg caggctcgag cattgatctg catggggggc aactttgctc
     1561 tggcaatgcc agatcgggaa gcgagcgctg taccgttaac acaattagat ctgacggtac
     1621 acgtagccac taagcttaac cgctctcatc tgctgaccgc acggcatagc tatattctgc
     1681 cggttctggg acgcagcgag attgacatgc aaaaaagcgg tgcgcaggcg gtaaccgttg
     1741 aggattcaat gtcgatgatt catgcctcgc gtggcgtgtt aaaacccgcc ggtgtaatgc
     1801 tgaaatcaga gtgtgcagtg gtcgcgggaa tcgcgcaggc agcactaccc cagagcgtgg
     1861 tagcctggga gtatctggtg gaagattatg atcgcattcg caatgacatt gaagctgtgc
     1921 tgccagagtt cgccgactat aaccagcgca tccgtcatcc cggtggtttt cacctgataa
     1981 atgcagctgc tgaaaggcgc tggatgacgc cgtcaggtaa ggctaatttc attaccagta
     2041 aagggctgtt agaagatccc tcttcagcgt ttaacagtaa gctagtcatg gcgacagtac
     2101 gcagtcacga tcagtacaac acgacgattt atggtatgga tgatcgctat cgaggggtat
     2161 tcggtcaacg agatgtggtc tttatgagtg ctaaacaagc taaaatttgc cgtgtaaaaa
     2221 acggcgaaag agttaatctt attgcgctta cgccagacgg taagcgaagc tcacgccgca
     2281 tggatagatt aaaagtggtc atttacccta tggctgaccg ctcactggtg acctattttc
     2341 cagaatcgaa tcacatgcta acacttgata accacgatcc attaagtggc attcctggct
     2401 ataaaagtat tccggttgaa ttagaaccat caaattaatt cgcctcctca tttcttctgc
     2461 tgtcatccgc acagcagaag aattcctcat tgaccattgt cgcgcaattt actcacatgt
     2521 gctaaattaa actgctcgtt ataagatata tacctccgcc tacagctgta agaaactccg
     2581 ctcagtactg aagcgccagt cctatttcct ctattctcca gcctgttata ttaagcatac
     2641 tgattaacga tttttaacgt tatccgctaa ataaacatat ttgaaatgca tgcgaccaca
     2701 gtgaaaaaca aaatcacgca aagagacaac tataaagaaa tcatgtctgt aattgtggtt
     2761 gtcttattac tgacacttac gttgatagcc attttttcgg caattgatca gctgggtatt
     2821 tcagaaatgg gtcgcattgc aagggatctt acacatttca ttatcaatag tttgcaagac
     2881 t
//
LOCUS       DACBVI010000273         2874 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.277, whole genome shotgun sequence.
ACCESSION   DACBVI010000273 DACBVI010000000
VERSION     DACBVI010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.277"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(6..82)
                     /locus_tag="IU325_22535"
     tRNA            complement(6..82)
                     /locus_tag="IU325_22535"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46..48),aa:Asp,seq:gtc)
     gene            complement(215..946)
                     /gene="dnaQ"
                     /locus_tag="IU325_22540"
     CDS             complement(215..946)
                     /gene="dnaQ"
                     /locus_tag="IU325_22540"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAP5811754.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGETTIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            1011..1478
                     /gene="rnhA"
                     /locus_tag="IU325_22545"
     CDS             1011..1478
                     /gene="rnhA"
                     /locus_tag="IU325_22545"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HAP5811755.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1475..2197)
                     /locus_tag="IU325_22550"
     CDS             complement(1475..2197)
                     /locus_tag="IU325_22550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP5811756.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGLPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2231..>2874
                     /gene="gloB"
                     /locus_tag="IU325_22555"
     CDS             2231..>2874
                     /gene="gloB"
                     /locus_tag="IU325_22555"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HAP5811757.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVKKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINV"
BASE COUNT          764 a          706 c          683 g          721 t
ORIGIN      
        1 gaatttggcg gaacggacgg gactcgaacc cgcgaccccc tgcgtgacag gcaggtattc
       61 taaccgactg aactaccgct ccgcgttgtg ttccgttggg aacgaggcga atattacgga
      121 ttgcctcacc cttcgtcaac ggtttttctc atcttttgaa tcgtttgctg caaaaatcgc
      181 ccaagtcgct atttttagcg ccttttacag gtatttatgc ccgccagagg cagcttccgc
      241 cctttttctg caccagatcg agacgggctt catgtgccgc aagctcttca tctgtcgcaa
      301 aaacaacgcg taacttactt gcctgacgca caatgcgctg aattgttgtt tcaccttgtt
      361 gctgttgtgt ctctccttcc atcgcaaaag ccatcgacgt ttgaccacca gtcatcgcca
      421 gataaacttc cgcaaggatc tgggcatcga gtaatgcccc gtgcaacgtt cgtttactgt
      481 tatctatttc gtagcgggca cataacgcat cgaggctgtt gcgcttaccg ggaaacattt
      541 tcctcgccac cgcaaggcta tcggtgacct tacagaaggt attggtcttc ggaatatcgc
      601 gcttaagcaa cgaaaactcg tagtccataa agccgatatc gaacgctgcg ttatggatca
      661 ccaactccgc gccgcgaata tagtccatga actcatcggc tacttcggca aacgtgggct
      721 tatcgagcaa aaattcatcg gcaataccat gtacgccaaa ggcttccgga tccaccagcc
      781 gatcgggttt gagataaaca tggaagttat tgcccgtcag gcgacggttc accacttcaa
      841 cggcaccaat ctcaatgatc ttgtggcctt catagtgcgc gccaatctgg ttcataccgg
      901 tggtttcggt atcgagaacg atctggcgtg taattgcagt gctcatagcg gtcatttatg
      961 tcagacttat cgttttacag ttcgattcaa ttacaggaag tctaccagag atgcttaaac
     1021 aggtagaaat tttcaccgat ggttcgtgtc tgggtaatcc aggacctggg ggttacggcg
     1081 ctattttacg ctatcgcgga cgcgagaaaa cctttagcgc aggctacacc cgcaccacca
     1141 acaaccgtat ggagttgatg gccgctattg tcgcgctgga ggcgttaaaa gaacattgcg
     1201 aagtcatttt gagtaccgac agccagtatg tccgccaggg tatcacccag tggatccata
     1261 actggaaaaa acgtggctgg aaaaccgcag acaaaaaacc agtaaaaaat gtcgatctct
     1321 ggcaacgtct tgatgctgcg ctggggcagc atcaaatcaa atgggaatgg gttaaaggcc
     1381 atgccggtca tccagaaaac gaacgttgcg atgaactggc tcgtgccgca gcgatgaatc
     1441 ccacactgga agatacgggc tatcaagtcg aagtttaagc ctgtggttta cgacattgcc
     1501 gggtggctcc aaccgcctgg cgaattcgtg gcttgttttt actctgtttc atcggattta
     1561 gcgttaaagg aatagtccgt ttccgggcga caataagttg taagcaacca agcgcaggaa
     1621 tatgcgcatt caatagtttt ccaccgtgtt tgttccacgg gagaacgtgg aaacggctgg
     1681 cgtgtagcac ttcaaaattc aacaaagaga gccagtccag ctgccgcatc agagtaaaca
     1741 tccggctatt atagggcgag gttttgcgca ataccggcac aagttttcgt aatcccatta
     1801 aactgatggg attgaagcca ctaatcacca gccagccatc atcaatcaat acccgatcgg
     1861 cttcacgcaa taaacgatgc ggatcggtgc accacggcaa tgtatgtgcc agtagacaaa
     1921 catcaacgga tttatcggca aaaggaagat gaagtgggtc cgcctgcacc tggacgggca
     1981 gtccttgcgc agaaacattc acttgatgag aaaccgcgca cgcttcgcaa ttgatttctg
     2041 cgcttaaatt gccaatctta agcagatgaa aaccatacat tttagtgaac cacgggttga
     2101 gctggcgctc cagcgccttg cgataaagct ctccccaggg caaatcgccc cagcaatcag
     2161 gagccacgac agtttgaggg actcttgccg gtttcatcac aaccttccgt ttcacactga
     2221 gaggtaatct atgaatctta acagtattcc cgcctttgat gacaattaca tctgggtttt
     2281 gaatgatgaa gcaggtcgct gcctgattgt cgatcccgga gacgcagagc cagtattaaa
     2341 cgccattgcc gccaataact ggcaaccgga ggccatattt ctcacccacc atcaccacga
     2401 tcacgttggc ggcgtaaaag aactggtgaa aaagtttccg caaattgtgg tgtatggtcc
     2461 acaagagaca caagataagg gaacaacaca ggtagtcaaa gatggcgaaa ctgccttcgt
     2521 tttggggcat gaatttagtg taattgccac accgggtcac actttaggac acatctgtta
     2581 cttcagtaaa ccttatctat tttgcggcga cacgctgttt tctggtgggt gtggtcggtt
     2641 gtttgaaggg acaccatcac aaatgtatca atcacttaaa aagttaagtg cgttacctga
     2701 cgatacattg gtatgttgtg ctcatgaata taccttatca aatatgaagt ttgctttgag
     2761 tattcttccg cacgatttgt ccataaatga ttattatcgt aaagttaagg agttacgggc
     2821 aaaaaatcaa ataacactac ccgtaattct gaaaaatgag cggcaaatta atgt
//
LOCUS       DACBVI010000274         2601 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.278, whole genome shotgun sequence.
ACCESSION   DACBVI010000274 DACBVI010000000
VERSION     DACBVI010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.278"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(120..338)
                     /locus_tag="IU325_22560"
     CDS             complement(120..338)
                     /locus_tag="IU325_22560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811758.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITILVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLINRLRERLVSHRDQQ"
     gene            complement(470..1993)
                     /locus_tag="IU325_22565"
     CDS             complement(470..1993)
                     /locus_tag="IU325_22565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAP5811759.1"
                     /translation="MRNTLIPIFIAICLFITGVTILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGTVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNYRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(2329..2577)
                     /locus_tag="IU325_22570"
     CDS             complement(2329..2577)
                     /locus_tag="IU325_22570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811760.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVLMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT          781 a          576 c          537 g          707 t
ORIGIN      
        1 caactgtgat aacaccaggg ttgacaacta tttgcgcaat aattcctttc cttcaatgat
       61 ccaggcgttc actgtcagca ccccattcag ccgcatactg acagtgagaa actgaaagat
      121 tattgctgat ccctgtggct gacgagtctt tcccttaatc gattaatcag attgatcgcc
      181 agccaaacaa gggcattaag caaatttatc gtcacaagca cttctcccag cccggtactg
      241 ggttttgcaa taaggatgac caggatagtg atcagcataa atgcaatgga aaagatgata
      301 tagagagtga aataatccag attattacta ttgttcatgc tactaatggc aatcctgtca
      361 aaactttcaa acaattatag caaacaaaat aatgaacaat agtaacaaga ggcaacgagc
      421 taattagctg tctataatta caccgcacta tggccttact gatcgatttt tactcaacca
      481 caaccttcac cttcggttta gaaaggataa ttttgaccag gtcgatgtat gttaccggtt
      541 tatagaaata ataaccctgc tgaaatgtga tattattttg attcagatag tcaagttgtt
      601 ctttcgtttc gacaccttca gcgacgatac tgatcgaaag tttacgcgcc agatccaata
      661 cacaatccag aatccgagtt gattctgggt ctgcgtttac gcggccaacg aaactgtgat
      721 caattttgat ataatcaata tgcaggtcat gcagataaga aagccctgag tagccagtac
      781 cgaaatcatc cagcgcgatg acaaaaccat tttcatgcag aatgttcaac cgctgaacca
      841 gactttcatc aacatttaat ggctcacgct cggtaacttc cagaacgagg tttaaatcgc
      901 gacgggtaaa actgtcacgg taatttaagc actcgtcgac aaatgtcggc gaaataatat
      961 gcgaggcgct aaaattaatc ccaatatgaa aaccttccgg cagtttactc gcgatagcgt
     1021 tcatctgtct ggcaacctga ttcatcaggc tttgcgtaag cggaacgatt aatccggatt
     1081 tttcagcaag tggaataaat gccgcgggtg atatatatcc accgtgaggt tgtttccagc
     1141 gggctaacac ctcaactccc cgcaatgtcc cttcccgacc atttaccaca ggttggtaaa
     1201 atgggacgat ctccccatta tttatagccc gtcgcaggat ctcttcaggc gttgcgcttt
     1261 tattgaaata cctatcaagc aggaaggcag cggcgcaggc gattaacaga ataaatatca
     1321 aaatcccaaa gcctttatcg ataagtctgt agaagctaaa gagtggcggc gggttaaaga
     1381 taatagtgaa atgatatttt gtggagtgaa ccttctcggt atagggatag tgtccattaa
     1441 gcgtcatcac atcacccgta ggtccaataa tgtcatctgc cacgcgtaat acatacctta
     1501 cccctttcaa gggtacatta agcgccccgc gaatatgctg atcacttatc gtaaccaaaa
     1561 tgcgcgtatc tgcaaattgg ctctgataga gcaggatagg taatctatta acggtgtcga
     1621 ttgccggagc cagcagtaaa ttactgtccg ggaaaacagg aatacgagac agtaggaccc
     1681 gattccccgg taaggatgta caccagaccg ttccctgttt gagaatgatg attgtgcgca
     1741 ggtgaggttt cagagctgct tcagtgccaa gctgatattg ctcctcgagg tcgcattcct
     1801 tcccggcaat gttaaccgcc gtttgagtcg cctgtgacgc ttcttcaagt atatgattga
     1861 tattgttggc tgcatatctt gctcccgcca ggtactctgc ttttgcggaa taccagagct
     1921 ggatgttcaa aatagtgaca ccggttataa acaagcatat cgcaatgaag atgggtatga
     1981 gtgtattgcg catttgcttg tcccggccaa agggtttaat acatgaaaat gccattcatt
     2041 cgctcgcact ggcatctaca gagagcatgg tagagagcat aagaaatatc cacgccgaag
     2101 ataaaatttt ctccatcttc atctaaccta ttaattattg agcacaatgt agtcagaatt
     2161 aaacaaatta ggtgggatat caggcgtcaa gaatggaaaa cgagcgctct ccattcctgt
     2221 ttgccccctg atattgcgga tatattaaga aagcataacg cctgaaatgc tcactttgca
     2281 ccagcatgat gatacagctg atgtttattc tataacctta ctcaagttct aagagagcac
     2341 tgattccctg tcattaataa tgatatccgt tagtgtagtc ttatgcattt caatgtactg
     2401 caagatttgc agatagagat cagaaagaat cacactagtt tcatctttta aacgcaagcg
     2461 taaagcctga tcaataatat gactatcaac atacccgtgc gtcatgagca cctccctctc
     2521 gagattttta aacaaagcac attgaaaaat aaatctctcg gggattgaat tattcatgtc
     2581 agcctcgtag gaatattgac c
//
LOCUS       DACBVI010000275         2550 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.279, whole genome shotgun sequence.
ACCESSION   DACBVI010000275 DACBVI010000000
VERSION     DACBVI010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.279"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            428..571
                     /locus_tag="IU325_22575"
     CDS             428..571
                     /locus_tag="IU325_22575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HAP5811761.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            584..793
                     /gene="cspE"
                     /locus_tag="IU325_22580"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             584..793
                     /gene="cspE"
                     /locus_tag="IU325_22580"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAP5811762.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            959..1768
                     /gene="rlmA"
                     /locus_tag="IU325_22585"
     CDS             959..1768
                     /gene="rlmA"
                     /locus_tag="IU325_22585"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HAP5811763.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1765..2331)
                     /gene="mntP"
                     /locus_tag="IU325_22590"
     CDS             complement(1765..2331)
                     /gene="mntP"
                     /locus_tag="IU325_22590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HAP5811764.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          668 a          599 c          615 g          668 t
ORIGIN      
        1 gatttttttg cgcattcatc aatttttctt attttaaatt tacaatacct ttggcgattg
       61 acttctcttt ggggtgatcg atagccgtta actgactgtt ttatgagaaa aagtgatata
      121 actttttatt cattgcatag cagaaaatgt gatattgcac gcactatgta ataacttctc
      181 ccactggcct ggaacaactg aacttattga actatgttag aaaatacgtc agtttaagta
      241 tctgcctgaa ctggcaaggt taagcacaat gatatatcgg cgcgtattcc gttgcataag
      301 tgtgcaaaaa aagtggaaga cgtatcgaga tttgtgcgtc tgatcgagac atgtttaaaa
      361 atggcttgcc ataattaacg ttgtatgtga taacagattt cgggttaaac gaggtacagt
      421 tctgtttatg tgtggcattt tcagtaaaga agtcctgagt aaacacgttg acgttgaata
      481 ccgcttctct gccgagcctt atattggtgc ctcatgcagt aatgtgtcag ttttatctat
      541 gttatgcctg cgggcgaaga aaacaatcta aggaattttt caaatggcaa agattaaagg
      601 tcaggttaag tggttcaacg agtctaaagg ttttggcttc attactccgg ctgatggcag
      661 caaagacgtg ttcgtacact tctctgcaat ccagggtaat ggtttcaaaa ctctggctga
      721 aggtcagaac gttgagttcg aaattcagga cggtcagaaa ggtccggcag ctgttaacgt
      781 aacagctatc tgatcgaatc cactgatctg aagtgtgaat acgcttcaat ctcgctataa
      841 agcctcgtcg aatgcgaggc tttttacttt gctttatctt cgctccagtc gttcggatat
      901 ttgcccgccg cgtgattcgc gttacacttg cggcctttag tatcctgccg gagttgtcat
      961 gtcgttttcc tgtccacttt gccatcagcc tctttcgcgt gaaaaaaaca gctatatctg
     1021 tccccagcga catcagtttg atatggcgaa agaagggtat gtcaatctgc tgcccgttca
     1081 gcataaacgg tctcgtgatc cgggcgacag cgcggaaatg atgcaagcac gccgcgcatt
     1141 cttagatgcc ggacattatc agccgctgcg tgatgcaatt gtcgctcaac tgcgggaacg
     1201 gcttgatgaa aaggccacag cggtgctgga tattggctgt ggtgaagggt attacacaca
     1261 cgcatttgcc gatgcgttgc ccgaaatcac cacgtttggc ctggatgtct cgaaggtagc
     1321 gataaaagcg gcggcgaaac gctatccgca ggtcactttt tgtgttgctt ccagccaccg
     1381 tttgccgttt tccgatacca gtatggacgc cataatacgt atttacgcgc cgtgtaaagc
     1441 agaagaatta gcgcgagtag tgaagcctgg cggttgggtc attactgcca cgccgggacc
     1501 gcgacatttg atggagctga aggggctgat ttacaatgaa gtacatcttc atgcacctca
     1561 tgcagaacaa ctggaaggtt ttacattaca gcagagtgct gagttgtgtt atccgatgcg
     1621 tcttcgcggt gatgaagccg tcgcattatt gcagatgacg ccgtttgcct ggcgtgcgaa
     1681 gccagaagtc tggcaaacac tggcagcaaa agaagtgttc gactgccaga cggactttaa
     1741 tattcacctc tggcagcgtt cttattaacc gtggaagtgc gtccagagga tctggacgcc
     1801 gatgccgatc agcaccagcc cgccgagaat ttccgctttt ttcccaataa ttgagccgat
     1861 aaagcgacca accatcatcc ctaatgtcga cataatcaag gttgcacaac caatggccaa
     1921 tgcggtcgcg ataatgttga cctgaaggaa agcaagacca acacccacag ccatggcatc
     1981 caggctggtg gcaatcgcgg tggttaccag tagccagaaa ccgtgtcgac ggcgcggctc
     2041 ttcatcttca tcatctgcgc cacgaaaacc ctcaataatc attcgcccgc cgaggaatat
     2101 cagcagcaca aacgcaatcc agtggttcca ttcaagaaca aaccggctgg ctaacatgcc
     2161 cattccccag ccgatcagcg gcgtcagggt ttcgacggca ccaaaaataa ggccggttcg
     2221 caacgcttca gaaaatttcg gtttatggag ggtggcacct ttaccgattg atgcagcaaa
     2281 tgcatccatc gacataccaa acgcaagaag aacagtagca gtgatattca tgacaacgtc
     2341 ctgaccgggg tatccataaa acacatcatc gcccccagca aacatgcata catgaacaac
     2401 aagccgcgat ggttttcgcg ttcatgcagg catagcgatt gatgtgcata tggtctcgcc
     2461 tgactgaaag tgttcagtcc gtacgtgcca cgttttgcaa cgagtatgtt gacacgtaca
     2521 tttccggaat ctggaaatcg gctactcccc
//
LOCUS       DACBVI010000276         2535 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.280, whole genome shotgun sequence.
ACCESSION   DACBVI010000276 DACBVI010000000
VERSION     DACBVI010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.280"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..196
                     /locus_tag="IU325_22595"
     CDS             <1..196
                     /locus_tag="IU325_22595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAP5811765.1"
                     /translation="LKLLFMECNPIKEAFLMPWTLTGICYEISQRKYIVTNPDDYDKY
                     SDMVKKYVIDGEDHLIKYYM"
     gene            complement(271..942)
                     /locus_tag="IU325_22600"
     CDS             complement(271..942)
                     /locus_tag="IU325_22600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP5811766.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEISGKEASEIATNGCVPANQFTWHSVSRAVGNVKNQGAAL
                     IQPVC"
     gene            complement(1051..1284)
                     /gene="yedF"
                     /locus_tag="IU325_22605"
     CDS             complement(1051..1284)
                     /gene="yedF"
                     /locus_tag="IU325_22605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HAP5811767.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1281..2486)
                     /gene="yedE"
                     /locus_tag="IU325_22610"
     CDS             complement(1281..2486)
                     /gene="yedE"
                     /locus_tag="IU325_22610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HAP5811768.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
BASE COUNT          685 a          657 c          624 g          569 t
ORIGIN      
        1 tttaaaatta cttttcatgg aatgtaatcc tattaaagag gcatttttaa tgccatggac
       61 tcttacaggg atttgctatg aaatatcgca gcggaaatat attgttacga accccgatga
      121 ttatgataaa tattccgata tggttaaaaa gtatgtaata gatggtgagg atcacctaat
      181 taaatattat atgtaagtaa ttaggcgcaa ttgtgtcact ggaatagagg tttcttgtga
      241 cacattttag ttcatcaata atctccaggg ttaacagaca ggttgaatta acgctgcgcc
      301 ctggttttta acattaccca ccgcgcgcga taccgagtgc caggtgaact ggttagctgg
      361 cacacagcca ttggtagcaa tttctgatgc ttctttaccg ctaatctctt gccgcatcca
      421 ttcccgcgca gcttctggcg acagtaccag tgggcggcgg tcatgaatat ctaccagacc
      481 ttgatctgcc gcagcagtga caatcaaaaa tccttcggct tcgtcaccac gctcaaatgg
      541 tgtgctacct atcgcggcca taaaaatagg ttgtccatca gcacgataga taaaaaaagg
      601 ctgttttttg tcgccttctt ttttccactc aaaccagcca tcggcaaaac agattgcccg
      661 accatgttgc cagagcggtt taaacatacg actggttgcc gcagtttcta cgcgggcgtt
      721 aatcagcggc ggtttatccc accatccggg agcatatccc cagaaaaccg gatccagatg
      781 aaggtgttca tcacgttcac tgagcagtag gactttggtt cccggcgcga cgttgtatct
      841 gccaatgggt tcgggatcat agggaatatc gcgttcaata tcttcagcga gaagcgcaag
      901 gtaatcttca cgcgtttggg attgggcaaa gcgtccacac atagaaacct cctgagagca
      961 cactgaaaag aataggagag ggggcgggta aaatctatgt ggtacagagg caacgatatt
     1021 cccgctgtgg gccacagcgg gaatgaggga ttacttctga attaaataac gaatggtcgg
     1081 cccgtcttgc tgaatatcca gcaccgtata gccgtgatta cgcgcatcca gtggaatatt
     1141 attgatcgac tgcggacagt cgctcaccac ttccaggatt tccccttttt ttaactgcgg
     1201 catcgcctca agggttgcga ctgccggata agggcagggt tcacccacca tatcaaggcg
     1261 gtaatcagga acgatatttt tcatgcgatc tccttagcag tctgcggtgc cgcacggcgg
     1321 aagaagcgtt tttcccagcc gataatcaac atcagcgcag caaacaacag caaatatgtc
     1381 accagcaggc cacccatcgg accaaaggtt ttcagcaggt tgattttgtc ccagtcggtg
     1441 gccagcgccg gagcgaaatc atcccagtaa tacgccagaa tcgttgagcc gatcacattg
     1501 cccagaccta cccaccagta gtgcacctgg ccttctaccg cgcggtacat ccagccggtt
     1561 tcgcagccgc cagccagcac gatgccaaaa ccaaacagta aaccaccaat taccgcgttt
     1621 ggtcccgccc acatgatttt gggttcaaca cctaactgta cgtaactgaa gatcccgatg
     1681 gcactcaccg ccataccaat aatgattgct ttcgccatat gggtacgtcc ggtgatccac
     1741 atatcgcgga acgccgaagt aaagcagatt tgcgcacgtt caatcagtaa accaaagccg
     1801 acgccaaaca gcattgccag ccccagtttg ggttggttca tcgctgtgag cagcgcccag
     1861 cccagcatgc cgaaaaagac cagcatcccg agacgaaaac gacgccgcgc ctgatccggt
     1921 ttttgcgtca gcggtgaggc ggcagaaact ttctgcattt tcacgggaat acggaagatg
     1981 ggcagcaggg taaagcgcgc gccaaaccat gaaccaatgg cagtggcgat ggcaaagaac
     2041 caggcatgca gcgagaactg aggaataccg gtaaagaacg ccgccaggtt acagcccatt
     2101 gccagacgcg cgccaaaacc ggcgataatg ccgccaatga tggcctgcat aatgcggata
     2161 cggctgcgcg gcattcgcag tttgacattg ttggcccaca gcgctgcggc aaagcagccg
     2221 ccaaacatac cgaggatcat catcccgtcg atgcgggtta atggcgatcc ttccagatgg
     2281 ataattttaa agtaacccca ctcttcagca tggacgccga acagttgcag gagctggccg
     2341 ccccaacggg taaattcacc cgtgacagcc caaaaggtgc cagtaatgcc aaaatagtaa
     2401 gtggagagaa tacccgccgc gatgaccgcg gggatgggcg cccagaattt aatcaaccag
     2461 gcgtgtttga attgctgcca tgacatgaat gtagcctctg aactcagaag gtttgaaaca
     2521 agagtcgcga atcat
//
LOCUS       DACBVI010000277         2534 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.281, whole genome shotgun sequence.
ACCESSION   DACBVI010000277 DACBVI010000000
VERSION     DACBVI010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.281"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(113..298)
                     /gene="rzoD"
                     /locus_tag="IU325_22615"
     CDS             complement(113..298)
                     /gene="rzoD"
                     /locus_tag="IU325_22615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HAP5811769.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSGNDW"
     gene            complement(515..1048)
                     /locus_tag="IU325_22620"
     CDS             complement(515..1048)
                     /locus_tag="IU325_22620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HAP5811770.1"
                     /translation="MNAKIRYGLSAAVLALIAVGAPAPDILDQFLDEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMRLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            1154..1426
                     /locus_tag="IU325_22625"
     CDS             1154..1426
                     /locus_tag="IU325_22625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811771.1"
                     /translation="MDNSTISLQELLDCISKLRDDVNALTVAFSYLAFSIPKEQMQPT
                     LASLQLESLNPKWSQQQQNSFKWLAVLLEEKYAGEITISAESSVNQ"
     gene            complement(1392..1736)
                     /locus_tag="IU325_22630"
     CDS             complement(1392..1736)
                     /locus_tag="IU325_22630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HAP5811772.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEILDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDEQSPEKRPIILEAQGVWITYKGKLPG
                     RITGSLKTPPKW"
     gene            complement(1741..1956)
                     /locus_tag="IU325_22635"
     CDS             complement(1741..1956)
                     /locus_tag="IU325_22635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP5811773.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(2106..2267)
                     /locus_tag="IU325_22640"
     CDS             complement(2106..2267)
                     /locus_tag="IU325_22640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811774.1"
                     /translation="MILRHRTGPAFQAIKDGLAVGLNALTLTDITKNATYGVEIESLV
                     LEINTPASS"
     gene            complement(2264..2467)
                     /locus_tag="IU325_22645"
     CDS             complement(2264..2467)
                     /locus_tag="IU325_22645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HAP5811775.1"
                     /translation="MVERTMAFKHYDVVRAAAPSDLAEKLTHKLKEGWQPFGSPVAIT
                     PYTLMQAIAAEGDVVVSGATEPE"
BASE COUNT          627 a          697 c          531 g          679 t
ORIGIN      
        1 tcactgttcc ttatgaaaca atcatcaaaa tgaatcatat tgtagttaag attttacttt
       61 aaacactgtt cggttatgta ttgctgagca ccttcaagtt gggcctgcat cattaccagt
      121 cgttcccgga gggtgaaata atcccgttca gcggtgtctg ccagtcgggg ggaggctgca
      181 ttatccacgc cggaggcggt ggtggcttca cgcactgact gacagactgc tttgatgtgc
      241 aaccgacgac gaccagcggc aacatcatca cgcagagcat cattttcagc tttcgcatca
      301 gctaactcct tcgtgtattt tgcatcgagc gcagcaacat cacgctgacg catctgcatg
      361 tcagtaattg ccgcgttcgc cagcttcagt tctctggcat ttttgtcgcg ctgctctttg
      421 taggcggtgg cgttatcacg gtaatgatta acagcccatg acaggcagac gatgatgcag
      481 ataaccagag cataaataat cgcggcgact ctgctcactg atctatcccc caacaggcta
      541 atgcgctttc ctggtcacga cgaataacct gtccatagca gttatttgaa cgtatgcggc
      601 aatcgcgccc accatctttt atccaccagc gaatcgcctc gcatgcaccc ttacgatcac
      661 cggcattcag ccgcttataa aacgtcgacg ggaaacactt accggggcca atgttatagg
      721 gacaaaatga cgctataccc gctttctgtg gttcggtcag tggtacttta atattgcgct
      781 ccacccatgc cagcgcctta tcacgttcaa tagcgttaac ctggtcgcat ttttccttcg
      841 acagcctcat tcccggtatg acgggcttac catccaccat tgtggcacca cgacagatgg
      901 tccatatacc ggaaccatcg cggtatgccg ttgtgtggtt accttctttt tcatccagaa
      961 actggtcgag aatatcaggc gcaggcgcac cgacggcaat cagtgccaga acggcagccg
     1021 acaggccgta tctgattttt gcgttcatgg atatttatca ggatttatcg gtttctgaac
     1081 cctggatatg tttatctgtc ccggcctgtt gaatcaggca aggaatagtt aaatacaata
     1141 gagaggattg tttatggaca atagcaccat ttctctacag gagttgctcg actgcatttc
     1201 caagcttcgg gatgatgtaa atgcccttac tgtcgcattt tcatatctgg cattctcaat
     1261 tcccaaggaa caaatgcaac caacactggc atcgctccag cttgaatcac tcaaccccaa
     1321 atggtcccag caacaacaaa attctttcaa gtggctggcg gtattactgg aagaaaaata
     1381 tgctggtgaa attaccattt cggcggagtc ttcagtgaac cagtaattct tcccggcagt
     1441 tttcctttgt aggttatcca cacaccctgc gcctctaaaa ttatggggcg cttttccggt
     1501 gactgctcat ccccttcaca taaccctgca gcaacatcca ggaagacctg tctgatgctc
     1561 cttctggctg ctgcctcata aaactccagc gcggcacctt caacacggtc cagcgagatg
     1621 tccaggtcta aaatttcacc gtcaaagcgt tttttgtccc gtaacgctaa agttaccgta
     1681 actttattct caaaattgcg gatccctttc acaatcagtt catagttttg agtcattgaa
     1741 ttactctccc cgtgccgcct tacgacggtc ctctctgatt ttgaaataca ggttagtcag
     1801 atatgtcagc agcccaaaca gcagactccc cagcacgcct attgccgccc actgagacgg
     1861 ggaaaccctg tccagcaact gcaggaacca gtagcccgtt cccaccgctg acgtggtgta
     1921 tgacacacct gttgtgattt tttccatctg gtccataccc cgtctcccgt tatccggaag
     1981 ctgacaacaa taaaaaagcc accagttaac tactgatggc tctgataact catgcaagcg
     2041 tctcagacga tccactgaca ctaccggtga gtttaacgat accttccatt tgactggctc
     2101 actttttatg atgatgccgg tgtatttatc tccagcacca gactttctat ctcaacgcca
     2161 tacgttgcat ttttggtaat atccgtcagc gtcagtgcat ttagtcccac tgccagaccg
     2221 tcttttatgg cctggaatgc cgggccagta cgatgacgta gtatcactcc ggctcagttg
     2281 caccactgac caccacatca ccttctgctg caatcgcctg catcagggta taaggggtta
     2341 tggccaccgg actaccaaac ggctgccagc cctctttcag tttatgtgtc agcttttccg
     2401 caagatctga cggcgccgcc gccctgacaa catcatagtg tttaaatgcc atggttcttt
     2461 ccaccatctg aaaaataatt ctttaaaata cctgacatgt aatacagaaa aaacacaaaa
     2521 ccatacctta aata
//
LOCUS       DACBVI010000278         2501 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.282, whole genome shotgun sequence.
ACCESSION   DACBVI010000278 DACBVI010000000
VERSION     DACBVI010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.282"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..255
                     /locus_tag="IU325_22650"
     CDS             <1..255
                     /locus_tag="IU325_22650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811776.1"
                     /translation="ECEGKYTVRNLVKNKAIALELAEMYVKNRYGHDAAEEEKPYKIM
                     ELPTSWIVEGTIESDQIAGRVFIIEIGKNDGRILNFGHGK"
     gene            360..1201
                     /locus_tag="IU325_22655"
                     /pseudo
     CDS             360..1201
                     /locus_tag="IU325_22655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
     gene            1249..1908
                     /locus_tag="IU325_22660"
     CDS             1249..1908
                     /locus_tag="IU325_22660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HAP5811777.1"
                     /translation="MLDRLESEILADRVSEESRRWLASCGLTVKQMQNQMDPVYTPAR
                     KIHLYHCDHRGLPLVLISTEGATEWCAEYDEWGNLLNEENPQQLQQLIRLPGQQYDEE
                     SGLYYNRHRYYDPLQGRYITQDSIGLKGGWNFYEYPLNPVQYIDPFGLADMNFYDEGI
                     RRNVSLHAATDKINIPGVFTIGGHGYPGFITDENGNYETAEGLAKRIRANPRYKKGMS
                     V"
BASE COUNT          820 a          428 c          573 g          680 t
ORIGIN      
        1 gaatgtgagg ggaaatacac cgttagaaat ttggtaaaaa ataaagctat agcattggaa
       61 ttagctgaga tgtatgttaa aaatcgttat gggcatgatg ccgcagaaga agaaaaacca
      121 tacaaaatca tggaattacc aacaagttgg attgttgaag gcaccattga atcagaccaa
      181 attgctggta gggtttttat tatagaaata ggcaaaaatg acgggagaat tctaaatttt
      241 ggccacggaa aataagtatg taaggataaa caaacatcgt gtgtgctgat ggcagagtag
      301 gtggaggact ccagacagtc aaacgataga aaaagatagt ctttatggag gttcctgcaa
      361 tgtcaaatac ataccagaaa agaaaggcaa gtaaagagta tggtttatat aatcaatgta
      421 agaaactaaa tgatgatgaa ttatttcgct tacttgatga tcacaattcc ttgaaaagga
      481 tttcatctgc cagagtatta cagttaagag gtggtcaaga cgctgttaga ttggcaattg
      541 agttctgctc tgataaaaat tatatccgta gagatatcgg agcatttata ctcgggcaaa
      601 taaaaatttg caaaaaatgc aaagataatg tgtttaatat tttgaacaat atggcattga
      661 atgataagag cgcttgcgtt cgagctacgg caatcgagtc aacggctcag cgatgcaaga
      721 aaaacccaat ttattcacct aaaatagtag aacaatctca aattactgct tttgataaat
      781 cgactaatgt cagacgtgct acagcatttg ctatttctgt tatcaatgat aaagcaacaa
      841 ttccactatt gattaatctg ttaaaagatc caaatggaga tgtcagaaac tgggccgcat
      901 ttgcaataaa tatcaataaa tatgataata gtgatattag ggattgtttt gtggagatgc
      961 ttcaggataa aaatgaggaa gtccgtattg aagcaataat cggactttcc tacagaaaag
     1021 ataaaagggt tttatctgtt ttatgcgatg agttaaaaaa aatactgttt atgatgatat
     1081 cattgaagct gcgggtgaat taggtgataa aacgctactt cctgttttag atactatgtt
     1141 gtacaagttt gatgacaatg aaattataac ttccgctatt gataagctga agcgttcata
     1201 gcgtgagttg cctatgcaca gtgggggatt cccgccggca cggtgcagat gctcgaccgg
     1261 ctggaaagtg aaatcctggc tgaccgggtg agtgaggaaa gccgccgctg gctggcatcg
     1321 tgcggcctga cggtgaagca gatgcaaaac cagatggacc cggtgtacac gccggcgcga
     1381 aaaatccacc tgtaccactg cgaccatcgc ggcctgccgc tggtgcttat cagcacggaa
     1441 ggggcaacag aatggtgcgc agaatacgat gaatggggca acctgctgaa tgaagagaac
     1501 ccgcaacagc tgcagcaact catccgcctg ccgggtcagc agtatgatga ggagtccggc
     1561 ctgtattaca accgccaccg ctattatgac ccgctgcagg ggaggtatat cactcaggat
     1621 tcgattgggc tgaagggggg atggaatttt tatgagtatc cgttgaatcc ggttcagtat
     1681 atagaccctt ttggattagc tgatatgaat ttctatgatg aggggatacg cagaaatgta
     1741 tcactgcatg ctgcaacaga taaaataaat attcctggcg tttttactat tggaggacat
     1801 ggatatcctg gttttattac agatgaaaat ggaaactatg aaactgctga gggattagcc
     1861 aagagaatca gagctaatcc acgctataaa aagggaatgt ctgtttgatt attctcatgt
     1921 ggtacagcca gagagggaga taaatctttt gccagcaagt tagccagagc acttcatgca
     1981 aatgtcactg gagctgatac acaatggcaa gtatggatgg cgtgggataa taatactcct
     2041 ctttttaata cgatccaaac aggacttacg gataatatct cggcaattga cacaagtaat
     2101 aatgtcataa ctggtaataa tatgggccgc tggataacgt ttggaccttc aggtcaaccc
     2161 atttcttcca ctcctggcaa agttatgaga ccaagtgata taaaataaca aaggtggcaa
     2221 catgaagtgc tttttattta ttctgattgc aaatgttttc tttttctctt gttgttcata
     2281 tggaggtgaa atcccccccc ctattaagct ccataacatt gaaataagct caacatctga
     2341 atcgtttaat ttaagtgaaa atcaaagata tgaacttgat attaaatctg agggcgggga
     2401 tggtgatgcg tcatatcgat tacatcaata ttatacatat gtttgcttta atatacccaa
     2461 gcaaattaaa tatctggcaa ggtctgcatc tcaaggaaat a
//
LOCUS       DACBVI010000279         2421 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.283, whole genome shotgun sequence.
ACCESSION   DACBVI010000279 DACBVI010000000
VERSION     DACBVI010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.283"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            67..555
                     /gene="eco"
                     /locus_tag="IU325_22665"
     CDS             67..555
                     /gene="eco"
                     /locus_tag="IU325_22665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HAP5811778.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRIWKAEEKIDNAV
                     VR"
     gene            complement(705..2351)
                     /gene="mqo"
                     /locus_tag="IU325_22670"
     CDS             complement(705..2351)
                     /gene="mqo"
                     /locus_tag="IU325_22670"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HAP5811779.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          570 a          674 c          604 g          573 t
ORIGIN      
        1 tgatgaatat gttaaccttc agcgacatca tcggtgaaaa cctataaatg aagaaggaaa
       61 gcaaaaatga agaccattct acctgcagta ttgtttgccg ctttcgccac cacctccgcc
      121 tgggcggcag agagcgtcca gccactggaa aaaatcgcgc cttatccgca agctgaaaaa
      181 ggcatgaagc gtcaggtgat tcagttaacc ccgcaagaag atgaatctac cctgaaagta
      241 gaactgttaa tcggtcagac gctggaagtc gattgcaatt tgcatcgtct cggcgggaag
      301 ctggaaagca aaacgctgga aggctggggc tatgattatt atgtctttga taaagtcagt
      361 tccccggttt cgacgatgat ggcctgcccg gatggcaaga aagagaagaa atttgtcacc
      421 gcgtatctgg gcgatgccgg aatgctgcgt tataacagca agctgccgat cgtggtgtat
      481 acgccagaca atgtggatgt gaagtaccgc atctggaagg cggaagagaa aattgacaac
      541 gcggtagtcc gctaaactgc cgtgaagtgc tccaccccgt aggtcggata aggcggtcac
      601 gccgcatccg acatctaacg cccaagccgg ttgcctgatg cgacgctggc gcgtcttatc
      661 agcatacgcc acatccggca taccatgccg gatgtggcac atcattacaa cgcaatatcc
      721 gccacttctt tttgaacggg ttgcggtttc aactgcggtt tcggcgtact atctgctgct
      781 tgcggtttgt cgtatttcag ccccagcact tcgctggtgt actgcaactc gcgctccgtt
      841 gccgctacat caccgttcag cttgcgtcca taagacggaa cgatcgcctt caacgttgcc
      901 tgccattgcg ggctggaaac gcgatcgcca aatacttttt ccagcagatt caacataatc
      961 ggcgcggcgg ttgacgcacc tggcgatgcc cccagcagcg cggcaatagt cccttgctgg
     1021 tcgctgacca cttcggtacc cagacgcagt acgccacctt tctcggcatc acgcttgata
     1081 atctgcacac gctgccccgc ttgccacaaa cgccagtcct cttttttcgc ctgcggatag
     1141 tactctttca acgcttcaaa acgatcctct tcactcaaca tcacctgact caccagatat
     1201 ttcaccagat cgaaattatc cagcccaacg tgcatcatcg gcatcacgtt agaggtggtg
     1261 gtggaactca tcagatccca caatgaaccg tttttgagga atttggttga gaaggtggca
     1321 aatggcccaa acagcactac gcgtttaccg tccagaacac gggtatcgat atgcggaacc
     1381 gacatcggtg gtgcgccaac ggatgcttta ccgtaaacct tcgccagatg gtgattaacc
     1441 acgtccgggt tttccgaaac aaggaactgt ccgcccaccg ggaaaccggc gtagtctttc
     1501 gcttccggaa tacccgattc ctgtaacaac ttcagcgccg caccgcccgc gccgataaag
     1561 acaaatttag cgcggatgtt ctgtgcagtg ccgtttttca gatcggcaac agtgacggtc
     1621 caggtgttat cgtcattacg ctttagggcg cggacttcgc tgctgagttg cagcgaaaaa
     1681 ttagatttct tctgcaagga agcaattaac tggcgggtga tctcgccata gttcacatca
     1741 gtaccgattt ccgtacgcgt ggctgccact ttctgttgcg gatcgcgccc ttccatcact
     1801 aacggtgccc actctttgat ctgcgcgtga tcttcagagt aacgcatacc acgaaacagc
     1861 gagctttgtt gtaacgcggc gtaacgggcg cgcaggaaat tgacgttatc ctcgccccag
     1921 acaaagctca tatgcggaac ggtattgata aatgaacgcg gagtacgcag cacgccgcgc
     1981 tcgacctggt gcgcccagaa ctggcgggaa atctggaatg cttcgttaat ggcgactgct
     2041 ttttcaatac tgatgctgcc atcggcgttt tgcggagtgt aattgagttc catcagtgca
     2101 gaatgcccgg ttccggcgtt gttccagccg ttcgaactct cctgcgcgac accctccagg
     2161 cgctccacca tggtcatcga ccattcaggc tccagctcgc gtaaataggt ccccaacgtg
     2221 gcgctcataa tgccgccgcc aatcaacagt acatcagttt cctgctcctc ggacgctttc
     2281 gcttttgccg ccatcgaaac ggcattaagg cccacggcca tcgagaagag catggcagtc
     2341 acttttttca tcttgttaat gccttacttt tagtcgcttt attgcaggtg agatttgcgc
     2401 ggcatcaacg gtaacattta a
//
LOCUS       DACBVI010000280         2339 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.284, whole genome shotgun sequence.
ACCESSION   DACBVI010000280 DACBVI010000000
VERSION     DACBVI010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.284"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            172..675
                     /locus_tag="IU325_22675"
     CDS             172..675
                     /locus_tag="IU325_22675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP5811780.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHVYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(721..1137)
                     /gene="rraB"
                     /locus_tag="IU325_22680"
     CDS             complement(721..1137)
                     /gene="rraB"
                     /locus_tag="IU325_22680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HAP5811781.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1299..2303
                     /gene="argF"
                     /locus_tag="IU325_22685"
     CDS             1299..2303
                     /gene="argF"
                     /locus_tag="IU325_22685"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP5811782.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
BASE COUNT          605 a          547 c          585 g          602 t
ORIGIN      
        1 atccattgat ataatttaca gatacagaca gtaaaatgtt ctgccacgtt acaatactct
       61 tatcagtaat aggctggcaa aaactttatt gctttataag acgcttgcgc acgtaagaag
      121 agagtggtat taaacaaatg tataattact ttatttttat tgggttaaac tatgaataac
      181 attgcgccgc aatcacctgt aatgcgtcgc ctgacgttgc aggataatcc tgctatcgcc
      241 cgcgtcattc gtcaggtatc cgccgaatac ggtcttaccg ctgataaagg ctacaccgtc
      301 gccgatccga atcttgacga gctgtatcaa gtgtatagtc agcctggtca tgtatattgg
      361 gtggttgagt acgagggtga agtggtcggc ggcggtggga ttgcgccatt agcaggtagt
      421 gaatcggaca tttgcgaact gcaaaagatg tattttcttc cggctattcg cggtaaaggg
      481 ctggcgaaaa aactggcctt aatggcgatg gagcaagcac gagagatggg cttcaaacgc
      541 tgctatctgg aaacgaccgc ttttttaaag gaagccattg cgctttatga gcatttgggc
      601 tttgagcata tcgactatgc gcttggctgc acgggccatg tcgattgtga agtgcggatg
      661 ctacgcgaac tctaaaaaaa tgcccgctag tgtgtgctat cgggcatttt aatcaattaa
      721 ttagtggcga accccgtcat cgtcttcatc gacaaaatct tcatcgtcgc cgtcttcgcc
      781 gttgggatct tcaaagtaag tgccccaacc gtcgtactca acgtcaaatt tctccgccag
      841 cgtcatcagt tgttcaacct gggcatcgat cagatcggca ttcaacgcgc actcgctgag
      901 gatgtcgcag caaatcacga tatcaccatc ttcaacttcc agctcttctg gatcggtcac
      961 ttcgtaaccg agtttaaacg cttcaactgc tgctttttcc agggtttcca gatcgtctgc
     1021 ggaaagatgg tgttcgatgg tatacagcgc gtccggatcg ctgccatctt ccagtaattc
     1081 ttcaataatc aaacgtgttt cttcacgctg ttcttccagt tgttccgggt ttgccatggc
     1141 tcattcctct taatgtcctg ccgatactct tattgtcaca caccactgac attgcctcca
     1201 cctttgtgac aaagatttat gctttagact tgcaaatgaa taatcatcca tataaattga
     1261 attttaattc attgaggtgt tagccacagg agggatctat gtccgggttt tatcataagc
     1321 atttcctgaa attactcgat ttcacgccag ctgaactcaa cagcctgctg cagttagccg
     1381 cgaagctgaa agccgataag aaaagcggca aagaagaagc caaactcact ggtaaaaaca
     1441 tcgcgctcat cttcgaaaaa gactcgaccc gtacccgatg ctctttcgaa gttgccgcat
     1501 atgaccaggg tgctcgcgtt acttatctcg gcccaagcgg cagtcagatt ggtcataaag
     1561 agtcgattaa agacactgcc cgcgtgctgg gtcgcatgta tgacggtatt cagtatcgcg
     1621 gctatggtca ggagattgtc gaaacactgg cggaatacgc tggcgtgccg gtatggaacg
     1681 gcctgaccaa tgagtttcac cccacgcagt tgctggcgga tctcctcacc atgcaggagc
     1741 atttacccgg taaagcattc aacgaaatga cgctggtcta tgcaggtgac gcacgtaaca
     1801 acatgggcaa ttcgatgctc gaagctgcgg cgcttaccgg tctggatttg cgtctggtcg
     1861 cgccacaggc atgctggccg gaagctgcgc tggttacgga atgccgcgcc ctggcacagc
     1921 aaaatggcgg gaatattacg ctgacggaag atgtagccaa aggagttgaa ggtgctgact
     1981 ttatctatac cgatgtatgg gtgtcgatgg gggaagcaaa agagaaatgg gcagaacgga
     2041 ttgcattact gcgtgattat caggtgaaca gcaagatgat gcagttgacc ggtaacccgg
     2101 aggtcaaatt cctccactgc ctgcccgcgt ttcatgacga ccagacgacg cttggtaaga
     2161 aaatggcgga agagtttggc ctacatggcg gaatggaagt gactgatgag gtcttcgaat
     2221 ctgccgccag cattgttttt gatcaggcgg aaaaccgcat gcataccatc aaagcggtga
     2281 tggtcgcgac gctcagtaaa taattgatat cgctataaag atgccgggaa atacaaaaa
//
LOCUS       DACBVI010000281         2259 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.285, whole genome shotgun sequence.
ACCESSION   DACBVI010000281 DACBVI010000000
VERSION     DACBVI010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.285"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            31..2028
                     /gene="betT"
                     /locus_tag="IU325_22690"
     CDS             31..2028
                     /gene="betT"
                     /locus_tag="IU325_22690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HAP5811783.1"
                     /translation="MNPVVFYTSAGLILLFSLTTILFRDFSALWIGRTLDWVSKTFGW
                     YYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFAAGIGIDLMFFSV
                     AEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMALGYFSYRYNLPL
                     TIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLNYGLSVLFDIPDS
                     MAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVLFMGDTSFLLNAL
                     VLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFVGLFLARISRGRT
                     IRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHPERGFYSLLAQYP
                     AFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWLRVFWSVAIGLLT
                     LGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRESANRDTAPRPLG
                     LQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRGAYVELKNLPPEE
                     GQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYRLETFLLEGSQGN
                     DLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
BASE COUNT          454 a          565 c          644 g          596 t
ORIGIN      
        1 gacctttcac acagcaggga aaaggacaaa atcaatccgg tggtgtttta cacctccgcc
       61 ggactgattt tgttgttttc cctgacaacg atcctgtttc gcgacttctc ggccctgtgg
      121 attggccgca cgctggactg ggtttctaaa accttcggtt ggtactatct gctggcggca
      181 acgctctata ttgtctttgt ggtctgtatc gcttgttcgc gttttggttc ggtgaagctc
      241 gggccagaac aatccaaacc ggaattcagt ctgctgagtt gggcggcgat gctgtttgct
      301 gccgggatcg gtatcgacct gatgttcttc tccgtagccg aaccggtaac gcagtatatg
      361 cagccgccgg aaggcgcggg tcagacgatt gaggctgcgc gtcaggcgat ggtctggacg
      421 ctgtttcact acggcttaac cggctggtcg atgtatgcgc tgatgggcat ggcgctcgga
      481 tactttagct atcgttataa tttgccgctc accatccgct cggcgctgta cccgatcttc
      541 ggtaaacgga ttaacgggcc gattggtcat tcagtggata ttgcagcggt aatcggcacc
      601 atcttcggta ttgccactac gctcggtatc ggtgtggtgc agcttaacta tggcttgagc
      661 gtactgtttg atattcctga ttcgatggcg gcgaaagcgg cactgatcgc cttgtcggtg
      721 atcatcgcaa caatctctgt cacctccggt gtcgataagg gcattcgcgt gttatcggaa
      781 ctcaacgttg cgctggcgct ggggttgatc ctgttcgtgc tgtttatggg cgacacctcg
      841 ttcctgctta atgcgctggt gctgaacgtt ggcgactatg tgaatcgctt tatgggcatg
      901 acgctcaaca gttttgcctt cgaccgtccg gttgagtgga tgaataactg gacgctcttc
      961 ttctgggcat ggtgggtggc atggtcgccg tttgtcggct tgttcctggc gcgtatctcg
     1021 cgtgggcgta ccattcgcca gttcgtgctg ggcacgttga ttattccgtt taccttcacg
     1081 ctgttatggc tctcggtgtt cggcaatagc gcgctgtatg aaatcatcca cggcggcgcg
     1141 gcatttgccg aggaagcgat ggtccatccg gagcgcggct tctacagcct gctggcgcag
     1201 tatccggcgt ttacctttag cgcctccgtc gccaccatta ctggcctgct gttttatgtg
     1261 acctcggcgg actccggggc gctggtgctg gggaatttca cctcgcagct taaagatatc
     1321 aacagcgacg cccccggctg gctgcgcgtc ttctggtcgg tggcgattgg cctgctgacg
     1381 ctcggcatgc tgatgaccaa cgggatatcc gcgctgcaaa acaccacggt gattatgggt
     1441 ctgccgttca gctttgtgat tttcttcgtg atggcggggt tgtataaatc tctgaaggta
     1501 gaagattacc gccgtgaaag tgccaaccgt gataccgcgc cgcgaccgct ggggcttcag
     1561 gatcgcctga gctggaaaaa acgtctctcg cgcctgatga attatccagg cacgcgttac
     1621 actaaacaga tgatggagac ggtctgttac ccggcaatgg aagaagtggc gcaggagctg
     1681 cggttgcgcg gcgcgtacgt ggagctaaaa aacctgccgc cggaagaggg acaacagttg
     1741 gggcatctgg atttgttggt gcatatgggc gaagagcaaa actttgtcta tcagatttgg
     1801 ccgcagcaat actcggtgcc gggctttacc taccgcgcac gtagcgggaa atcgacctac
     1861 taccggctgg aaaccttcct gttagaaggc agccagggca acgacctgat ggactacagc
     1921 aaagagcagg tgatcaccga tattctcgat cagtacgagc ggcaccttaa ctttatccat
     1981 ctccatcgtg aagcgccggg ccatagcgtg atgttcccgg acgcgtgatt gttattgcat
     2041 aaaaccgcgc catatctgca tatggcgcgg tttctcttac ctcttgatac gttatatctg
     2101 tgtacagtta agcccttagt atcgattgat gattgccagg caacagataa taaaaaagaa
     2161 aagaactatt gaagcccaaa acctacgctt ggactgttgt gaatgttcaa taagcttagt
     2221 cttatttaat gtaaatattg gtgctaattt gaaatgacg
//
LOCUS       DACBVI010000282         2245 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.286, whole genome shotgun sequence.
ACCESSION   DACBVI010000282 DACBVI010000000
VERSION     DACBVI010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.286"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            110..634
                     /gene="aroL"
                     /locus_tag="IU325_22695"
     CDS             110..634
                     /gene="aroL"
                     /locus_tag="IU325_22695"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HAP5811784.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            684..875
                     /gene="yaiA"
                     /locus_tag="IU325_22700"
     CDS             684..875
                     /gene="yaiA"
                     /locus_tag="IU325_22700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HAP5811785.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            1133..1810
                     /locus_tag="IU325_22705"
     CDS             1133..1810
                     /locus_tag="IU325_22705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811786.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLEKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLM"
     gene            1882..2166
                     /gene="ppnP"
                     /locus_tag="IU325_22710"
     CDS             1882..2166
                     /gene="ppnP"
                     /locus_tag="IU325_22710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HAP5811787.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          594 a          509 c          563 g          579 t
ORIGIN      
        1 taagtggaat tttttcttta caatcgaaat tgtactagtt tgatggtatg atcgctattc
       61 tcatgacacc ggctttcgcc gcattgcgac ctattgggga aaacccacga tgacacaacc
      121 tctttttctg atcgggcctc ggggctgtgg taaaacaacg gtcggaatgg cccttgccga
      181 ttcgcttaac cgtcgatttg tcgataccga ccagtggttg caatcacagc tcaatatgac
      241 ggtcgcggag atcgtcgaaa gggaagagtg ggcgggattc cgcgccagag aaacggcggc
      301 gctggaagcg gtaactgcgc catccaccgt tatcgccacg ggcggcggca ttatcctgac
      361 ggaatttaat cgtcacttta tgcaaaataa cgggatcgtg gtttatttgt gtgcgccagt
      421 atcagtcctg gttaaccgac tgcaagctgc accggaagaa gatttacggc caaccttaac
      481 ggggaaaccg ctgagcgaag aagttcagga agtgctggaa gaacgcgatg cgctttatcg
      541 cgaagttgcg catattatca tcgacgcaac aaacgaaccc agccaggtga tttctgaaat
      601 tcgcagtgcc ctggcacaga cgatcaattg ttgattttcg agcgcctata cttaacgttc
      661 atcccgtgaa ataaggaaga acgatgccaa cgaaaccgcc ttatcctcgt gaagcatata
      721 tagtgacgat tgaaaaagga aaaccaggac agacggtaac ctggtaccaa ctcagagccg
      781 atcatcctaa accagactcg ttgatcagtg aacatccgac cgctcaggaa gcgatggatg
      841 cgaaaaaacg ctatgaggac cctgacaaag agtgaccgca tcagactgct cggaagggat
      901 tctgagtgcc gccacaaggg atctgcgtca catttttcac aattcatgtt tttcaaataa
      961 ttagaatatt aaacaataac aatccattac tggaatcatt tggaatcttt acattatgcc
     1021 gtgcaggtct gctgctacgc tttttgtcat ttgtagcaca ggtaagtgtc agcagtggtg
     1081 cttagcgctt gcccggtaat taacgacgaa ataaaagtaa ggtggaagaa caatgagtgc
     1141 gtcgttggcg atcctgacca tcggcattgt acctatgcag gaagttttgc cgctcctgac
     1201 agaatacatt gacgaagata atatttccca tcatagcctg ctggggaagt taagccgcga
     1261 agaagtgatg gcggagtacg cgccagaagc aggcgaagac acaattctca cattattaaa
     1321 tgacaaccag ctggcccatg tttcgcgtcg caaagtggag cgtgacctgc aaggtgtggt
     1381 tgaagtgctc gataatcagg gttatgacgt cattttatta atgagtacag caaacattag
     1441 tagtatgact gcgcgtaata cgatctttct tgagccgtcg cgaatattgc ctccgctggt
     1501 ttcttctatt gttgaagatc atcaggtggg ggttatcgtt ccggttgagg agttgctgac
     1561 cgttcaggcg caaaaatggc aaattttgca gaaaccgcca gtattttcat tgggtaaccc
     1621 cattcatgat tcggaacaaa aaatcattga tgccggaaaa gaattactgg aaaaaggtgc
     1681 agatgtcatc atgctggatt gtttgggatt taaccagcgt catcgcgatt tactgcaaaa
     1741 acagctcgat gttcctgttt tgctctctaa cgtattgatt gcacggctgg ctgcggaatt
     1801 actgatgtaa ttttgcgtga cagccagcgc ctctggcccc tatagtgaag tagatgttca
     1861 actaccagac agggccagtt tatgcttcaa agtaatgagt acttttccgg caaagtgaaa
     1921 tcgatcggct tttccagcag cagcactggt cgcgccagcg tgggtgttat ggttgaaggt
     1981 gaatacacct tcagcaccgc tgagcctgaa gagatgacgg taatcagtgg cgcgctgaat
     2041 gtgttactgc ctgacgcgac cgactggcaa gtgtatgaag ccggttcggt gtttaatgtt
     2101 cccggtcaca gcgagtttca tctgcaagtt gccgaaccca cctcttatct gtgccgctat
     2161 ctgtaattcc tcgccttccc cttgaacggg agggcattta tctgaaatgt cctctcttta
     2221 acccataata atatttcctt tgctg
//
LOCUS       DACBVI010000283         2191 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.287, whole genome shotgun sequence.
ACCESSION   DACBVI010000283 DACBVI010000000
VERSION     DACBVI010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.287"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            175..2160
                     /gene="yjcS"
                     /locus_tag="IU325_22715"
     CDS             175..2160
                     /gene="yjcS"
                     /locus_tag="IU325_22715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HAP5811788.1"
                     /translation="MNNSRLFRLSRIVIALTAASSMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKVYYRADDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHADHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVKALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTLRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPEANASFYISREDLHAVLTGQVKMA
                     DLVKAKKAKIIGNGAILEEIIACLDNFDLWVNIVTPN"
BASE COUNT          578 a          570 c          545 g          498 t
ORIGIN      
        1 taataaattc gcgtactttt ccctcttcag cacaaatgcc taataaaatg gcgcgttcag
       61 ctcacacttc tacttattaa agtcgcaatt tcccaattat gataacgcca gcttcaaatg
      121 acgtattttg catgggttac atatttacga cataaatgat aaatggagat ttatatgaat
      181 aactctcggt tattccgttt gagcaggatt gttattgcgt taactgccgc cagcagcatg
      241 atggtaaata ccgctaatgc gaaagaggaa gcgaaagccg ccactcaata tacccaacag
      301 gttaatcaga attacgccaa atcattaccg tttagcgatc gtcaagattt tgacgatgcc
      361 cagcgtggat ttatcgcccc gctgctggat gaaggtattc tgcgtgatgc gaacggtaaa
      421 gtttactacc gcgcagacga ttacaaattt gatattaatg ccgctgcgcc ggaaaccgta
      481 aaccccagcc tgtggcgtca gtcgcaaatc aacggtattt ctggcctgtt caaagtcacc
      541 gataaaatgt atcaggtgcg cggccaggat atctctaaca ttacgttcgt tgagggcgag
      601 aaaggcatta ttgttatcga cccgctggtg acgccgcctg ccgcaaaagc cgcacttgat
      661 ctttacttcc agcatcgtcc gcaaaaaccg attgttgctg tgatttacac ccacagccac
      721 gccgaccatt atggcggtgt gaaaggcatt atctctgaag cggatgttaa atccggcaaa
      781 gtgcaggtga tcgctccggc aggctttatg gacgaagcca tcagcgaaaa cgtgctggcg
      841 ggtaacatca tgagccgccg tgcgctctac tcttacggtc tgttactgcc gcacaacgcg
      901 caaggcaacg tgggcaacgg cctgggcgtg acgctggcaa cgggcgaccc gagcattatt
      961 gcaccgacta aaactatcgt cagaactggc gagaagatga ttatcgacgg cctggaattt
     1021 gacttcctga tgactccggg tagcgaagcg ccagccgaaa tgcacttcta tattccggct
     1081 ctgaaagccc tgtgtaccgc cgagaacgcc acgcataccc tgcacaactt ctacactctg
     1141 cgcggcgcga aaacccgcga caccagcaag tggaccgagt atctgaacga aacgctggat
     1201 atgtggggta atgacgcgga agtactgttt atgccgcaca cctggccggt ctggggcaat
     1261 aagcatatca atgattatat tggtaaatat cgcgatacta tcaagtacat tcacgaccag
     1321 accctgcacc tggctaacca gggctacacc atgaatgaaa tcggcgacat gattaagctg
     1381 ccgcctgcgc ttgccaataa ctgggccagc cgcggctatt acggttctgt cagccacaac
     1441 gcccgcgcgg tgtataactt ctatcttggc tattacgacg gtaacccggc taacctgcat
     1501 ccgtatggtc aggtggagat gggtaaacgt tacgtgaaag cgctgggcgg ttctgcccgc
     1561 gtcatcaacc tggcgcaaga agcgaacaag caaggtgatt accgctggtc ggcagaactg
     1621 ctgaaacagg tgattgccgc caaccctggt gaccaggtcg cgaagaatct gcaagcgaat
     1681 aactttgaac agctgggcta tcaggccgag tccgccacct ggcgcggttt ctacctgacc
     1741 ggcgcgaaag agctgcgcga aggggtgcat aaattcagcc acggcaccac cggttccccg
     1801 gacacccttc gcggaatgtc ggtcgaaatg ctgttcgact ttatgtccgt tcgcctcgat
     1861 agtgcgaaag ccgcgggtaa aaatatcagc ctgaacttca atatgagcaa cggcgataac
     1921 ctcaacctga cgctgaacga tagcgtgctt aactaccgta aaacgctgca acctgaagcc
     1981 aatgcctctt tctacatcag ccgtgaagat cttcacgccg tgctgaccgg acaagtcaaa
     2041 atggcggatc tggtaaaagc gaagaaagcc aaaattattg gcaatggcgc gatactggaa
     2101 gaaattattg cctgtctgga taatttcgat ttgtgggtga atatcgtaac cccaaattaa
     2161 tgccactcac ttttgggggc gcttaagccc c
//
LOCUS       DACBVI010000284         2144 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.288, whole genome shotgun sequence.
ACCESSION   DACBVI010000284 DACBVI010000000
VERSION     DACBVI010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.288"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..758)
                     /locus_tag="IU325_22720"
     CDS             complement(<1..758)
                     /locus_tag="IU325_22720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAP5811789.1"
                     /translation="MKLGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSFQDDKISFTIETNHSEKYSMLSGMRAHILRR
                     NNSYQFIVTINSKNYGCPLDDTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVSDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLGQLLNIEDEVLNVLLRENFNE"
     gene            complement(1020..>2144)
                     /locus_tag="IU325_22725"
     CDS             complement(1020..>2144)
                     /locus_tag="IU325_22725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAP5811790.1"
                     /translation="IERNFFTLLSGNFKPDWRWAVSKETFTKEKFDSYVESVFSKIDF
                     YKQCGVINPQNANTAYFGDTDGRVGAVLYALLVSGHIGIRGEGWSLLCDLLKHEDMAS
                     FAYENKKLKKLFTLLDTRDMILNELHQHVFLKGDAITPCIFLGDHTGDRFSTIFGDKY
                     ILTLLNSMRNMEGNKDSRINKNVVVLAGNHEINFNGNYSARLANHKLSSGDTYDLIKT
                     LDVCNYDSETKVLTSHHGIIRNEENECYCLGALQIPFNQMKDPIDPEELANIFNKKHK
                     QHMDDRLIHLIRSNAIASTPVYDNYFNNTTAFRPKPEDIFKCGQTLKKSKQKYGHYGL
                     GVDQHQKIDNYTMGLNSWKIAPNERGDKKGVPGLSCFQSH"
BASE COUNT          662 a          417 c          342 g          723 t
ORIGIN      
        1 tgctcattaa agttctccct tagcaatacg ttgagaactt catcttctat attaagcaac
       61 tggccaagga aattttctcc ttcagccatg attcccaaat gtcccgttgc gaaaagcgcg
      121 tacaaaactg caccaactga gccatcggta tcgccaaaat aggttggtct ggtacttgtg
      181 ggatcaacag gtgaacaatt ggagaaatag gttctgtcag cgaactgggg ttttatcatt
      241 gtttcgaatt taatatcaga tacattgtat tgcgatattt tccagttcca gatgggtttg
      301 tacgactcag taactgcaac atcattagca ttatcattta ctgtcatatt attaagtaaa
      361 taaacaatgc tgcaccaatt aatatcggta tcatcaagag ggcaaccgta atttttacta
      421 ttaattgtca caataaactg ataactatta ttcctacgta gaatatgggc acgcatgccc
      481 gatagcatac tatatttttc tgaatgattc gtttctatgg taaatgaaat tttgtcatct
      541 tgaaaagatt gttggaaacg ttctatttgc caatcatcaa ttttttccac aacttcaccg
      601 agcattgtcc tggcaatact tgatatatca tcacctatac catttgcaat atacccaaac
      661 agttctttag tgaagttagt aacatgctct tgcaccatat catttgttgc gattgtcgtt
      721 gtactgtcgt tagtgcctgc cacagttcca agtttcatct tttacctcat ataattcacc
      781 gcaaggaata catagttatc atttaatctt atgacataca cctcatgttc gacggggaaa
      841 taattatatt ttgcacaaat cattcagcct taaagcataa tatgaaattt cttatctcat
      901 agtagagaaa gtccctaaag attgtagagg cgtcatcagt acatgcctct acaatcgatt
      961 aagaaaaact atgaaatact ggcaggatta aaaaaatata ccaccgtatt catggagttt
     1021 taatgagatt gaaaacaact aagccctggg acaccttttt tatcaccacg ttcgttaggt
     1081 gctatcttcc aggaatttag ccccatggta taattatcaa ttttttgatg ctgatctacc
     1141 cctaatccat aatggccata tttctgttta ctttttttga gcgtctgtcc acatttgaag
     1201 atatcttcag gttttggtct gaaagcagta gtgttattaa aataattgtc atatacaggt
     1261 gtggatgcta tagcattgct tctaattaga tggataagac gatcatccat gtgttgttta
     1321 tgttttttat taaatatatt tgctaattcc tcaggatcaa ttgggtcttt catctgatta
     1381 aatggaatct gcaaagcacc tagacaataa cattcatttt cttcatttct tattattccg
     1441 tgatgactgg ttaaaacttt cgtttctgag tcataattac aaacatcgag cgttttaatt
     1501 aagtcatatg tatcaccgga agagagttta tgatttgcta gtcgtgcact atagttacca
     1561 ttaaaattga tttcatgatt tccagccaga acgacgacat ttttatttat acgactatct
     1621 ttattaccct ccatatttcg catagaattt agcaaagtaa ggatatattt atcaccaaaa
     1681 atagtactaa atctatcgcc tgtatgatcc ccaaggaaga tacaaggggt aatcgcatcg
     1741 ccttttaaaa aaacatgttg atgcaactca ttcaagatca tgtctctcgt atctaataaa
     1801 gtgaagagtt ttttcaattt tttattttca tatgcgaatg aagccatgtc ttcgtgtttt
     1861 aataaatcac ataataaact ccacccctct cccctgatgc ctatatgccc tgaaacgagc
     1921 aatgcataaa gtacagcgcc tactcttcca tctgtatcgc caaaataagc cgtgttggca
     1981 ttttgtggat taatcacacc acactgttta taaaagtcta tttttgaaaa aacagattca
     2041 acatagctat caaacttttc cttagtaaac gtctctttag agacagccca acgccagtca
     2101 ggtttgaaat taccactaag aagagtaaag aaatttctct ctat
//
LOCUS       DACBVI010000285         1870 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.289, whole genome shotgun sequence.
ACCESSION   DACBVI010000285 DACBVI010000000
VERSION     DACBVI010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.289"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            230..412
                     /locus_tag="IU325_22730"
     CDS             230..412
                     /locus_tag="IU325_22730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811791.1"
                     /translation="MNINGKNASFTPEELIRKYHEEAPVDLKNNHGDKINEKNNNKHI
                     TYFPVDAILDGNKHLP"
     gene            468..>1870
                     /locus_tag="IU325_22735"
     CDS             468..>1870
                     /locus_tag="IU325_22735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III cell invasion protein SipB"
                     /protein_id="HAP5811792.1"
                     /translation="MSAPITGQTITFEQISETLRTQYSDAEKRLQDSNKTQVDPMRLN
                     KNPKSLDNDIRARLENKPMLAPPAMQVSDSDNATTAKTNDARLTMILGNLTGIADQDI
                     TTRLHNNLDSTLLRHEMAHNKFRELSDAYSSSLDSAQKADDIMHQANNNYNAVDKKVQ
                     SLEKKVNTLNQELSQLQPGDPQYNKVVTQKSAAEKTLTLSLQKKSLAEKSLNTAIMDA
                     DAAIGQSMEIFDEIQQQEQINNFTTNICLTQENQKNRNATATFILLITSVMEVIGDTN
                     CDSIKNQSEVMKEINHVRENKLNETARSYTTWTKVLNIINEVVTVVTFAVSAALIVIG
                     IVAAVPSAGASFAGALALIGGIAGAVVLGVDIICQITLGTTATGWILGKLTEGLAGII
                     SKYDPTLLAICALLDALGVQQTTIDLVQDIYSSAAASIVMVTLMIGATLICSVAISEL
                     VKALSKTAAEEITKEIT"
BASE COUNT          648 a          375 c          377 g          470 t
ORIGIN      
        1 cgacatgtat catatctgtt taatgctatc tcccgataac gatttctctt tggtttatta
       61 tatgggccaa tgccagatgg gcttaaaaaa cattaaaatg gccactgagt tatttaatac
      121 agtcgtaacc tacagccaaa atgaaaaagt taaagagatg gctactgctt atcttgaact
      181 cttgacagcc aataccgaag aggaagttca aacagagtga gtcggcaaaa tgaacataaa
      241 cggaaaaaac gcttctttta cgcctgaaga gttaatcagg aaatatcatg aggaagcccc
      301 ggttgattta aaaaataacc atggcgataa aataaatgag aagaataaca ataaacacat
      361 tacttacttt cccgttgatg ccattttaga tgggaataaa catctgccat gagttaaata
      421 aaaaagaatt aattacaccc gatgatagct taaaaaggag gtgaattatg tcggcaccga
      481 ttacgggaca aacgatcacg tttgaacaaa tcagcgaaac attgcgcaca cagtatagcg
      541 atgcggaaaa aagactgcag gacagcaaca aaacgcaagt tgatcccatg cggcttaata
      601 aaaacccgaa atcccttgat aatgatattc gcgctcgtct tgaaaacaaa ccgatgcttg
      661 ctccgcctgc aatgcaggtt tcagattccg acaacgccac tacagcaaag acgaacgatg
      721 cccgcctgac aatgattttg ggtaatctaa caggcattgc cgatcaggat attacaacac
      781 gtttgcataa taaccttgac agcaccctgc ttcgacacga aatggcacat aataagtttc
      841 gtgaattaag tgacgcttac tcatcgtcat tagattccgc gcaaaaagcc gacgacatca
      901 tgcatcaggc caataataat tacaatgcgg tcgataaaaa ggtgcaatcg ctggagaaaa
      961 aagtcaacac attgaatcaa gagctctcgc aactgcaacc tggcgatccg caatataata
     1021 aagtagtgac gcaaaaaagt gcggctgaaa aaacactcac gctgtcatta caaaaaaaat
     1081 cgttagctga gaaatcgtta aatacagcca ttatggatgc tgatgctgcg atcggccaaa
     1141 gtatggaaat ttttgacgaa attcaacagc aagaacagat taataacttc accaccaata
     1201 tttgcctgac gcaagaaaac cagaaaaaca gaaacgcgac agccacattt atccttttga
     1261 ttacctcagt aatggaagtt attggtgata ccaattgcga ctctattaaa aatcaatctg
     1321 aggtaatgaa agagattaac catgtcaggg aaaataaact caatgaaaca gcgcgtagct
     1381 atactacatg gacaaaagtt ttaaacatta ttaatgaggt cgttactgta gttacatttg
     1441 ccgtaagtgc agccttaatt gtgattggta ttgtcgcagc agttccaagt gctggggcaa
     1501 gctttgctgg agcgctggca ctaattggag gaatcgcggg ggcagtagta ttaggtgttg
     1561 atattatttg tcaaatcact ctgggtacca cggctaccgg ttggattttg ggaaaactaa
     1621 ctgaaggtct tgcgggtatt atttctaaat atgaccccac actactcgca atctgcgctc
     1681 ttctggacgc cttgggtgtc caacaaacga ctattgattt agtacaagat atttactcat
     1741 ctgcagccgc ttctattgtt atggtaacat taatgattgg tgcaacatta atttgttcag
     1801 tagcaataag tgaacttgta aaggcactct caaaaactgc cgccgaagaa atcacaaaag
     1861 aaataactga
//
LOCUS       DACBVI010000286         1866 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.290, whole genome shotgun sequence.
ACCESSION   DACBVI010000286 DACBVI010000000
VERSION     DACBVI010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.290"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            70..1320
                     /locus_tag="IU325_22740"
     CDS             70..1320
                     /locus_tag="IU325_22740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811793.1"
                     /translation="MRNNKFKLKRITVVSALCFSVLYTNTAFAVWPVTEEVLTPYLMG
                     ASPQSMGNGIIETLVSMNDKLAQATITANENQKNAVVSNASQAIQEQRMKNILNRIPD
                     ANACEEVTSAGGRGAAGSNTSSAKEALSENSVKMFTQANTGINEAKKNIMGRVSISVC
                     SEDDVKYNRGGCNTIGQYPDKDKSASSLTTPPLSKSDALSNKVSNQSYSVEQIEVAEA
                     AKRNLIGNLPVEPLKNKGLEDTTEGREYLSAFSSFVSRSTAGTNAIDSVLAMKKASNV
                     SVNSRGAKIGSTLGSTQGANLAWSDASVKNKYATLFPGTTFPDNPSEWEMLRYEIYSR
                     YADTTGADAWQVKISSADEKETAHEQARMQALDLRLQMLQIERTEDTNILLAALLGQQ
                     LQPVDKQMLNNLKTNATRATQATK"
     gene            complement(1383..1790)
                     /locus_tag="IU325_22745"
     CDS             complement(1383..1790)
                     /locus_tag="IU325_22745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HAP5811794.1"
                     /translation="MSDALKIINNIRTLRAQARELTLETLEEMLEKLTIVVEERREEE
                     ASFLKQQEEKQAKLEAFRQKLLEDGIDPAELLSTVNSSSSKTGAKRAPRPAKYKYIDT
                     DGSEKTWTGQGRTPRAIAAALEAGKALEDFEIK"
BASE COUNT          612 a          359 c          412 g          483 t
ORIGIN      
        1 ttaaaattga cagatcacat ttaattagtt aaataataaa ttaggtaata agttagcggg
       61 agtattgtaa tgaggaacaa taaatttaaa ttaaagagaa taacagtggt ttcggctttg
      121 tgtttttctg tgttatatac aaatacagct tttgctgtct ggcccgtcac agaagaggtt
      181 ttgacaccgt atcttatggg ggcttcacct caatctatgg gcaatggaat aattgaaaca
      241 ttagtttcaa tgaacgacaa gctggcacaa gcaacaataa cagcaaatga gaatcagaag
      301 aatgctgttg tcagtaatgc cagtcaggca atccaagaac agcgcatgaa aaatatctta
      361 aacagaatac ctgatgccaa tgcctgtgaa gaagtcacga gcgcaggtgg tcgtggtgca
      421 gcaggatcga atacaagctc agcgaaagag gctttatctg aaaacagcgt taagatgttt
      481 acacaagcaa ataccggaat aaatgaggcc aagaaaaaca tcatgggcag agtatcaata
      541 agtgtttgct ccgaagatga tgtgaaatat aacagaggtg ggtgcaatac tataggtcag
      601 tatcctgata aagataagtc ggcatcaagc ctaactacac caccattatc taaatccgat
      661 gcattgtcta ataaggtttc gaatcagtcc tattctgttg agcagattga agttgctgaa
      721 gcggcaaaaa gaaatctgat agggaactta ccagttgaac cattaaagaa caagggatta
      781 gaagatacaa ctgaaggacg agaatattta tcggcctttt cgagctttgt ctccagaagc
      841 actgctggaa caaacgccat tgattccgta ctggcaatga aaaaagcaag taatgtatca
      901 gtgaactcaa gaggagccaa aataggatcc acattaggtt ctacgcaggg agcaaacctt
      961 gcttggtcag atgcttccgt aaaaaacaag tatgcaactc tatttcccgg cacaacattc
     1021 ccggataatc cttcagagtg ggaaatgtta agatatgaga tctattcacg ctatgcagat
     1081 acaacaggtg ctgacgcttg gcaggtaaaa atcagtagtg ctgatgaaaa agaaactgcg
     1141 catgagcagg caagaatgca ggcacttgat ttaagattac aaatgcttca aatcgaaaga
     1201 acagaagaca caaatatttt gcttgcggct ctgttaggac aacaactaca accagttgat
     1261 aagcaaatgc tgaataattt aaaaacaaat gcgacaagag cgactcaggc aacaaaataa
     1321 ttgaagtcat taaaaaaacc gccagcgctg ggccagcgga tttggtagac ttggtatgaa
     1381 gattatttga tctcgaaatc ttccagcgcc ttaccggctt cgagcgcagc agcgatagca
     1441 cgaggagttc ggccctgacc tgtccatgtc ttttcagaac catcggtatc gatgtattta
     1501 tacttagctg gacgtggggc gcgtttcgct ccggtcttgg aagaagatga gttaacagtt
     1561 gaaagcaact ctgccgggtc tatgccatct tccagtaact tctgacggaa tgcttccagc
     1621 ttggcctgtt tctcttcctg ctgctttaga aatgaggcct cttcttcacg acgttcttct
     1681 acaacgatag tgagtttttc taacatctct tccaaggttt cgagtgtcag ttcacgagcc
     1741 tgagcacgga gagtacgaat attgttgatg atctttaaag catcagacat gcgatttccc
     1801 taatttttgt ggtaaagaat acaagcaata acatcatagt tttaatcgtt cctgtgcaca
     1861 aaatca
//
LOCUS       DACBVI010000287         1861 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.291, whole genome shotgun sequence.
ACCESSION   DACBVI010000287 DACBVI010000000
VERSION     DACBVI010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.291"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            38..907
                     /locus_tag="IU325_22750"
     CDS             38..907
                     /locus_tag="IU325_22750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP5811795.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKDVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAKAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(1067..1660)
                     /gene="rclC"
                     /locus_tag="IU325_22755"
     CDS             complement(1067..1660)
                     /gene="rclC"
                     /locus_tag="IU325_22755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HAP5811796.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(1672..>1861)
                     /locus_tag="IU325_22760"
     CDS             complement(1672..>1861)
                     /locus_tag="IU325_22760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAP5811797.1"
                     /translation="AFSTNADDKIILKHISVSSVSASPTVLEDAIADIARKYNASSWK
                     VTSMRIDNNSTATAVLYK"
BASE COUNT          535 a          375 c          421 g          530 t
ORIGIN      
        1 gtagactcat tgttcatacc taatggaggg actgacagtg gaattttccg tattaagtaa
       61 caatctgaaa atgccgatga tgggatttgg tgtttttcag gttaccgata aagacgtgtg
      121 caaacagtca gtgttgaatg ctatccgcac gggttatcga ctcatcgaca ctgcagcggt
      181 atacggtaat gaagatgccg tcggtgaagc cgttcgtgaa gctatatctg aaggtttatg
      241 tacccgagat gagttattta taacgtctaa attgtgggtg caggatatgt tgaatcagga
      301 tacggcaaaa gcaggtattg aagcatcatt aaaaaaatcg ggactagaat acttcgacct
      361 ctatttattg caccaggcta tgcgcgatta tttcagtgcg tggcgtgcac tcgaagatgc
      421 ctatgaagag ggcaaattaa aagcaattgg ggtttccaat ttctatcctc atgttctggc
      481 gaacttttgt gaaacggtaa gagttaaacc gatggtcaac caggtcgagt tgcatcccta
      541 ttttgcccaa ccagaggcgc tggcaaccat gaagtattat aacgtgcagc ctgaagcatg
      601 ggctccgtta ggtggtggac gacataaacc ctttgaaaat aatctgcttc agagtattgc
      661 agatgcccat caaaaatcga tttctcaagt cattctgcgt tggaatattc aacggggagt
      721 ggtcgttatt ccgaaatcga cacatcaaca gcgtatcgaa gaaaattttg ctatctggga
      781 tttctcactg acagagaaag aaatggcaca aattagttcg cttgatttgg gttatgttgg
      841 ggagtcggta aaacatttta atcctgaatt tgttcgtggt tgtcttgctg taaaaataca
      901 tgattgatat taatcatgta ttttacctga gacgacaaga atcttttaac aggggagtga
      961 tattgatctt cactctgtca tatctccggt aatatggcgt caggcttcat catcagaaag
     1021 ggggcctgac gcctgaaaaa gtgaacaaca gacagtgttc ggattatcaa tattcagttt
     1081 ttaacgttga actggattca ttactgcgtt gtttaagaat ttcccgcgcc gaatctgcca
     1141 ttatcatgac agcacctgcc agcatcagag tatctttcaa taccaggcga ccagcaccgg
     1201 ataaataagg gaaaccatga tgagcgtcac ccaatgcggg tacccatgcc tccggggtgg
     1261 tgattaaaaa tgagagtgtt accaacggtg tggtaaatgc catcagccct cccaataaac
     1321 ctaaccagcg attgacagga ttagccaaaa ccagcagagc aataatcacc tccacgacgc
     1381 caagaccgtt ggaaaaacca taggtattat tggccgattg ccatgccctt gcttctggtt
     1441 tgtattcgcc ttcgtgagtc agatactgtt tatagtcttc cgggtgttca taaaagaacg
     1501 acattagtgg actgtttgcg acgaatggtg taatgctgtc tgcctcgtaa gggacaaact
     1561 ttaataaccc aatccacata aaaacaatcg caatactcag acgaatcaat gtcaggccaa
     1621 ttttatctcc ccgacttaac agatgcaggt atttttccat tgtgaacatc cttatttata
     1681 caatactgct gttgcggttg aattattatc aattcgcatc gatgtgactt tccaggatga
     1741 agcattatat tttctggcta tatcagcaat ggcatcctcc agaactgtcg gtgatgctga
     1801 tactgacgag acgctgatat gtttcagaat tattttatca tctgcattgg tggaaaatgc
     1861 c
//
LOCUS       DACBVI010000288         1819 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.292, whole genome shotgun sequence.
ACCESSION   DACBVI010000288 DACBVI010000000
VERSION     DACBVI010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.292"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            190..1212
                     /gene="lacY"
                     /locus_tag="IU325_22765"
                     /pseudo
     CDS             190..1212
                     /gene="lacY"
                     /locus_tag="IU325_22765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
     gene            1278..>1819
                     /gene="lacA"
                     /locus_tag="IU325_22770"
     CDS             1278..>1819
                     /gene="lacA"
                     /locus_tag="IU325_22770"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HAP5811798.1"
                     /translation="MNMPMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVIKDIPPNVVAAGVPCRV"
BASE COUNT          427 a          395 c          428 g          569 t
ORIGIN      
        1 ataattagaa ccccttttta ctacttttct tactattatt ttaccatata tcgagtcata
       61 aaagctgata atttaacata tttttgagca cttttctttc acccaatggg tgaaagctga
      121 atttcgaagg aatgcatatt tatcaaggct ttgattatgc tttttgaagt actgacgtag
      181 aatctagggt ggattattac cggcatgtta gtgatgtttg cgccgttctt tatttttatc
      241 ttcgggccac tgttacaata caacatttta gtaggatcga ttgttggtgg tatttatctt
      301 ggcttttgtt ttaacgccgg tgcgccagca gtagaggcat ttatcgagaa agtcagccgt
      361 cgcagtaatt tcgaatttgg tcgcgcgcgg atgtttggct gtgttggctg ggcgctgtgt
      421 gcctcgattg tcggcatcat gttcaccatc aataatcagt ttgttttctg gctgggttct
      481 ggctgtgcac tcatcctcgc cgttttgctc tttttcgcca aaacggatgc accctcttcc
      541 gccacggttg ccaatgcggt aggtgccaac cattcggcat ttagccttaa gctggcgctg
      601 gaactgttca gacagccaaa actgtggttt ttgtcactgt atgttattgg cgtttcctgc
      661 acctacgatg tttttgacca acagtttgct aatttcttta cttcgttctt tgccaccggt
      721 gaacagggta cgcgggtatt tggctacgta acgacaatgg gcgaattact taacgcctcg
      781 attatgttct tcgcgccgct gatcattaat cgcatcggtg ggaaaaacgc cctgctgctg
      841 gctggcacta ttatgtctgt acgtattatt ggctcatcgt tcgccacctc agcgctggaa
      901 gtggttattc tgaaaacgct gcatatgttt gaagtaccgt tcctgctggt gggctgcttt
      961 aaatatatta ccagccagtt tgaagtgcgt ttttcagcga cgatttatct ggtctgtttc
     1021 tgcttcttta agcaactggc gatgattttt atgtctgtac tggcgggcaa tatgtatgaa
     1081 agcatcggtt tccagggcgc ttatctggtg ctgggtctgg tggcgttggg cttcacctta
     1141 atttccgtgt tcacgcttag cggccccggt ccgctttctc tactgcgtcg tcaggtgaat
     1201 gaagtcgctt aagcaatcaa tgtcggatgc ggcgcgagcg ccttatccga ccaacatatc
     1261 ataacggaat gatcgcaatg aacatgccaa tgaccgaaag aataaaagca ggcaagctat
     1321 ttaccgatat gtgcgaaggc ttaccggaaa aaagacttcg tgggaaaacg ttaatgtatg
     1381 agtttaatca ctcgcatcca tcagaagttg aaaaaagaga aagcctgatt aaagaaatgt
     1441 ttgccacggt aggggaaaac gcctgggtag aacctcccgt ctatttctct tacggttcca
     1501 acatccatat aggccgcaat ttttatgcaa attttaattt aaccattgtc gatgactaca
     1561 cggtaacaat cggtgataac gtactgattg cacccaacgt tactctttcc gttacgggac
     1621 acccggtaca ccatgaatta agaaaaaacg gtgagatgta ctcttttccg ataacgattg
     1681 gcaataacgt ctggatcgga agtcatgtgg tcattaatcc aggcgtcacc atcggggata
     1741 attctgttat tggcgcgggt agtgtcgtca taaaagacat tccaccaaac gtcgtggctg
     1801 ctggcgttcc ttgccgggt
//
LOCUS       DACBVI010000289         1754 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.293, whole genome shotgun sequence.
ACCESSION   DACBVI010000289 DACBVI010000000
VERSION     DACBVI010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.293"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            53..250
                     /locus_tag="IU325_22775"
     CDS             53..250
                     /locus_tag="IU325_22775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811799.1"
                     /translation="MAAEHTRAEKDLAEQAKNVMHSVMSSYLDQINRDVQGRDQALNL
                     LQQIIQTIIDTDSAIVQGIRS"
     gene            294..1643
                     /locus_tag="IU325_22780"
     CDS             294..1643
                     /locus_tag="IU325_22780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IpaD/SipD/SspD family type III secretion system
                     needle tip protein"
                     /protein_id="HAP5811800.1"
                     /translation="MINKDNNLNIDILRQQNTNIPLKEASSQSEEPHQLENSKSEGNL
                     YNSASMDNNIGAMLKKDVEKLSTLVSERSPRNEDWKCQHDIQLNFVKNMLRNFPTENL
                     SEKGSALLHESYSLIKNVLQLTENVNTIDGKLKDPAKILTLHNSVRKTLANVADNTTG
                     SSAMASTKGTLNHAIASLDSSAPEVRSSATSSTLTASSSNEFKSDKYICSDIADLMSV
                     LGSDYLEIYANSVEIMSAYWQDFSEHIQSNMGKWTHSNKKGDAIVFDVNAFQKALMHF
                     YYIDKYPNGDFHYHYNPDYVLYPPAPADKIGVPLEEAEKWCAALGLPVIPPDPKHRTP
                     SPIVEVEPQGSGLYVIIPNPQIIDSMSQSSDSMVHRDDKGKEKNISKEFTGYEISTAE
                     YQAWLAGYNSQAENMKTDVQVITTKYSTANSTYDTIIKLLSSTITALFDSAKDYLRF"
BASE COUNT          605 a          355 c          333 g          461 t
ORIGIN      
        1 taccaataat ctatttcagt ctattgcaaa tctatctcaa tacggcggaa cagtggcagc
       61 agaacatact cgtgcagaaa aagacttggc tgagcaagct aaaaatgtta tgcattcagt
      121 tatgagtagc tatctggatc aaattaatcg tgatgttcaa ggaagagatc aagcactcaa
      181 tttattacag caaataatac aaaccattat tgatactgat agtgcaatcg ttcaaggcat
      241 tcgctcataa attattgcaa taatagttag ttatatttaa ggaggaatgg cacatgatta
      301 ataaagataa taatctaaat atagatattt tacgtcagca gaatacaaac attcctctta
      361 aagaggcatc ctcacaaagt gaggagcctc atcaactgga aaacagcaaa agcgagggta
      421 atttgtataa cagcgccagt atggataata atattggcgc tatgctcaag aaagatgttg
      481 aaaaactatc aactttagtc agtgaacgta gcccgcgaaa tgaagactgg aaatgccagc
      541 atgacataca actcaacttc gttaaaaaca tgctcagaaa tttcccaact gagaacctgt
      601 cagagaaggg atctgcgctc ttacacgaaa gttattcgct gataaaaaac gttctgcagc
      661 ttacagaaaa tgttaatact attgacggaa aattaaagga tcccgctaaa atattgacct
      721 tgcataactc tgtgagaaaa accctggcta acgttgctga caacacaacc gggtcaagtg
      781 caatggcatc gaccaaaggt accttaaatc acgctatcgc ttctttagat agttctgcac
      841 ctgaagtgag aagttcagca accagcagta ctttaacggc cagtagttca aacgaattta
      901 aaagtgataa atatatatgc agtgatattg ctgatttgat gagtgtgctc ggtagcgact
      961 acctcgaaat ttatgcgaat agcgttgaga taatgagcgc atactggcag gacttcagtg
     1021 aacatatcca aagcaatatg ggaaaatgga ctcactctaa taaaaaaggc gatgcgatag
     1081 tttttgacgt caatgcattc caaaaagcct tgatgcattt ttattatatc gataaatatc
     1141 ctaatggtga tttccactat cattacaatc cagactatgt tttataccct ccggcacctg
     1201 cggacaaaat tggcgttcca ctggaagaag cagaaaaatg gtgcgccgct ttaggacttc
     1261 cggtaattcc acccgatccc aaacaccgca caccttctcc gattgtcgaa gttgaaccgc
     1321 aaggtagcgg tctgtatgtt attattccca accctcagat tatagacagt atgagtcagt
     1381 cttcagactc aatggtgcat agggacgata aagggaagga aaaaaatata tctaaagaat
     1441 tcaccggcta cgaaataagc acggctgaat accaggcatg gctggcgggt tataacagcc
     1501 aggcagaaaa catgaaaacc gacgtgcagg ttatcacgac taaatacagt acagccaact
     1561 ccacctacga tacaattatc aaattattat cttcaaccat tactgcgttg tttgattcag
     1621 caaaagatta tttacgtttc tgattaagta acatccacaa ttatcgccca ctatctggtc
     1681 ccgaaaaatg ggaccagcgt ttcatattat taagcatcag ctttcattca tttcttcatc
     1741 acaatcttac aaaa
//
LOCUS       DACBVI010000290         1724 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.294, whole genome shotgun sequence.
ACCESSION   DACBVI010000290 DACBVI010000000
VERSION     DACBVI010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.294"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            80..187
                     /locus_tag="IU325_22785"
     CDS             80..187
                     /locus_tag="IU325_22785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811801.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGKQV"
     gene            393..1676
                     /gene="gltA"
                     /locus_tag="IU325_22790"
     CDS             393..1676
                     /gene="gltA"
                     /locus_tag="IU325_22790"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HAP5811802.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT          449 a          421 c          399 g          455 t
ORIGIN      
        1 acatttattt aatttttaat catctaattt gacaatcatt caacaaagtt gttacaaaca
       61 ttaccaggaa aagcatataa tgcgtaaaag ttatgaagtc ggtatttcac ctaagattaa
      121 cttatgtaac agtgtggaag tattgaccaa ttcattcggg acagttatta gtggtaaaca
      181 agtttaataa ttcggattgc taagtacttg attcgccatt tattcgtcat caatggatcc
      241 tttacctgca agcgcccaga gctctgtacc caggttttcc cctctttcac agagcggcga
      301 gccaaataac aaacgggtaa agccaggttg atgtgcgaag gcaaatttaa gttccggcag
      361 tcttacgcaa taaggcgcta aggagacctt aaatggctga tacaaaagca aaactcaccc
      421 tcaacgggga cacagctgtt gaactggatg tgctgaaagg cacgctgggt caagatgtta
      481 ttgatatccg tactctcggt tcaaaaggtg tgttcacctt tgacccaggc ttcacttcaa
      541 ccgcatcctg cgaatctaaa attactttta ttgatggtga tgaaggtatt ttgctgcacc
      601 gcggtttccc gatcgatcag ctggcgaccg attctaacta cctggaagtt tgttacatcc
      661 tgctgaatgg tgaaaaaccg actcaggaac agtatgacga atttaaaact acggtgaccc
      721 gtcataccat gatccacgag cagattaccc gtctgttcca tgctttccgt cgcgactcgc
      781 atccaatggc agtcatgtgt ggtattaccg gcgcgctggc ggcgttctat cacgactcgc
      841 tggatgttaa caatcctcgt catcgtgaaa ttgccgcgtt ccgcctgctg tcgaaaatgc
      901 cgaccatggc cgcgatgtgt tacaagtatt ccattggtca gccatttgtt tacccgcgca
      961 acgatctctc ctatgccggt aacttcctga atatgatgtt ctccacgccg tgcgaaccgt
     1021 atgaagttaa tccgattctg gaacgtgcta tggaccgtat tctgatcctg cacgctgacc
     1081 atgaacagaa cgcctctacc tccaccgtgc gtaccgctgg ctcttcgggt gcgaacccgt
     1141 ttgcctgtat cgcagcaggt attgcttcac tgtggggacc tgcgcacggt ggtgctaacg
     1201 aagcggcgct gaaaatgctg gaagaaatca gctccgttaa acacattccg gaatttgttc
     1261 gtcgtgcgaa agacaaaaat gactctttcc gcctgatggg cttcggtcac cgcgtgtaca
     1321 aaaattacga cccgcgcgcc accgtaatgc gtgaaacctg ccatgaagtg ctgaaagagc
     1381 tgggtaccaa agatgacctg ctggaagtgg ctatggagct ggaaaacatc gcgctgaacg
     1441 acccgtactt tatcgagaag aaactctacc cgaacgtcga tttctactct ggtatcatcc
     1501 tgaaagcgat gggtattccg tcttctatgt tcaccgtgat cttcgctatg gcacgtaccg
     1561 ttggctggat cgcccactgg agcgaaatgc acagtgacgg tatgaagatt gcccgtccgc
     1621 gtcaactgta tacaggatat gaaaaacgcg actttaaaag cgacattaag cgttaatggt
     1681 tgattgctaa gccgtttact ttccggaccc gcctttaata gagc
//
LOCUS       DACBVI010000291         1707 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.295, whole genome shotgun sequence.
ACCESSION   DACBVI010000291 DACBVI010000000
VERSION     DACBVI010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.295"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(605..1072)
                     /locus_tag="IU325_22795"
     CDS             complement(605..1072)
                     /locus_tag="IU325_22795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAP5811803.1"
                     /translation="MKACLLLFFYFSFIFQLHGADVKIKQNESMMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYCCFTKNNIYKQLRYLERSASQGSVTAQFNYGVFLSD
                     TNPILSEHYNLNKAIYWMEFAVNNGNIDAKSKLQDLKKLKLKRMDRRKNKENP"
     gene            complement(1084..1662)
                     /locus_tag="IU325_22800"
     CDS             complement(1084..1662)
                     /locus_tag="IU325_22800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HAP5811804.1"
                     /translation="MTQDPIGLKGGWNFYQYPLDPVINVDPQGLVDINLYPESDLIHS
                     VADEINIPGVFTIGGHGTPTSIESATRSIMTAKDLAYLIKFDGNYKDGMTVWLFSCNT
                     GKGQNSFASQLAKELHTNVIGPDTLWTWWGRGTNGKLKMDTVLTAPTNLNSNKDLMAI
                     TTKDLGNWITYGPSGHPISNMQGTPEKPSDIR"
BASE COUNT          499 a          365 c          290 g          553 t
ORIGIN      
        1 cgaattattt agtacaaaaa agcagcgtta cgtgatcttt ccctggcatg tcattagatg
       61 aaccagttat acaaccctcg gaacagaatc gaggtcctaa agacggacca aaagcgttta
      121 aacaactatg tattgaaaat ccattacgat tataagttcc tgtattaact gacggtacta
      181 atcgtatgcc gtcaggtttt ctattgcttg ctcccgggtc attaacattc caactccata
      241 ggccttgagg tattggccct ctatttataa atgtgtacaa cggggattat ttttacattg
      301 catattatta ccagaagcga ctggaatact aagcacatca taaaaattat catcacctgg
      361 tatatctgaa acacacgaaa gcatacctaa agttatgctg tataaacagg tggctaatcc
      421 caaggggatc gattccagaa atcgggttca acggatactg ataaaaattc cacctacctt
      481 ccagcccaat cggagcctga gtgttatacc gcccctgctg cggttaatgt taccgataat
      541 agttatagta cagccctgat tcatcatcat actactgctg cggcagtctg atgaattatt
      601 gcttttacgg attctcctta ttcttccttc tatccattcg ctttagcttt agctttttga
      661 gatcttgaag tttgctcttt gcatcaatat taccgttatt tacagcgaat tccatccaat
      721 aaatagcttt attcaaatta taatgttctg ataatattgg atttgtatcc gataaaaaaa
      781 ccccataatt aaactgcgct gtaacactcc cctgagatgc tgatctttct aagtatcgca
      841 attgtttgta aatgttattt ttagtaaagc agcaatattg atatagtcga aatgatgcct
      901 ctgaatcacc atgttgtgat ttatacttca gtttcatcaa cttttcttca cttaaatcat
      961 aggtcattgc tgtagacccc atcatacttt cgttttgttt tattttcaca tcagcaccat
     1021 gcaattgaaa aataaaagag aaataaaaaa atagtaacaa gcatgctttc atacatctac
     1081 aacctatctt atatcactgg gtttttctgg cgtaccttgc atattagaaa tggggtgccc
     1141 agatggccca tatgttatcc aattaccaag gtcttttgtt gttatagcca ttagatcctt
     1201 atttgaatta aggttcgttg gtgctgttag cactgtatcc atttttaact taccattagt
     1261 tcctcgcccc caccacgtcc atagcgtgtc aggtcctatt acatttgtat gtaactcttt
     1321 agctaattgg ctagcaaatg aattttgtcc tttacctgta ttacaagaaa ataaccaaac
     1381 tgtcatccca tctttataat tcccatcaaa tttaattaga tatgctagat ctttagctgt
     1441 catgatactg cgcgttgcgg attcaataga tgtgggggta ccatgccccc cgattgtgaa
     1501 aacgcctggg atattaatct catcagctac agaatggata agatcacttt cggggtataa
     1561 atttatatca accaaacctt gcggatctac atttatgacc ggatccaacg gatactgata
     1621 aaaattccat ccccccttaa gcccaatcgg atcctgagtg atataccgcc cctgcagcgg
     1681 gtcataatag cggtggcggt tgtaata
//
LOCUS       DACBVI010000292         1696 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.296, whole genome shotgun sequence.
ACCESSION   DACBVI010000292 DACBVI010000000
VERSION     DACBVI010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.296"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            69..1067
                     /locus_tag="IU325_22805"
     CDS             69..1067
                     /locus_tag="IU325_22805"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HAP5811805.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGVSQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            1079..1546
                     /locus_tag="IU325_22810"
     CDS             1079..1546
                     /locus_tag="IU325_22810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HAP5811806.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAAENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
BASE COUNT          459 a          415 c          417 g          405 t
ORIGIN      
        1 ttgaaatcag atttcgcata ttgaaatttt aagccaaaaa agcgatcaaa aaaacaagga
       61 agccagggat gaaagtgaca tttgagcagt taaaagcagc ctttaatcga gtcttaattt
      121 cacgcggcgt tgacagcgaa acggctgatg cctgtgccga gatgttcgcc cgcaccaccg
      181 aatccggcgt ttattctcac ggcgtcaacc gtttccctcg tttcattcaa caactggaaa
      241 acggcgatat cattcctgat gcccaaccca aacgtataac cagcctcggc gcaattgaac
      301 agtgggacgc ccagcgttcg atcggtaacc tgacagcgaa aaagatgatg gatcgcgcca
      361 tcgaactggc tgccgatcat ggtattggtc tggtggcact acgtaatgcc aaccactgga
      421 tgcgcggcgg cagctacggc tggcaggcgg cggaaaaagg ctatattggc atttgctgga
      481 ccaactccat cgccgtaatg ccgccgtggg gcgcaaaaga gtgtcgcatc ggcaccaacc
      541 cgctgatcgt cgccattcct tctaccccaa tcaccatggt cgatatgtcg atgtcgatgt
      601 tctcttacgg catgttagaa gttaaccgtc tggcaggtcg tcagctcccg gttgatggtg
      661 gctttgatga tgagggcaat ttgaccaaag aacctggcgt tatcgagaag aatcgccgca
      721 ttttgccgat gggctactgg aaaggttctg gcatgtcgat tgtgctggat atgatcgcca
      781 ctctcctttc cgacggcgca tcggttgccg aagtcaccca ggacaacagc gacgaatacg
      841 gcgtttcgca aatctttatc gccatcgaag tggataaatt gatcgacggc cccacccgcg
      901 atgcaaagct gcaacgcatc atggattacg ttactaccgc tgaacgcgct gacgaaaacc
      961 aggccatccg cttacccggc cacgaattta ctaccctgct ggccgaaaac cgccgtaacg
     1021 gcatcaccgt tgatgacagc gtgtgggcca aaatccaggc gttatgagga gataagtcat
     1081 gatttttgga catatcgcgc aacccaatcc gtgccgtttg cctgccgcca ttgaaaaggc
     1141 gctcgatttt ctgcgcgcta ccgatttcaa cgccctggag ccgggtgttg tcgaaatcga
     1201 cggcaagaat atttatgcgc aaattatcga tttaaccaca cgcgaagcgg cggaaaaccg
     1261 tccggaagtg catcgtcggt atatcgatat tcagtttctg gcgtggggtg aagagaaaat
     1321 cggcattgct attgatacgg gaaataataa agtcagcgaa tcactattag agcagcgcga
     1381 tattattttt tatcacgata gtgaacatga atcatttata gaaatgatac cgggcagcta
     1441 cgctattttc ttcccgcagg atgttcatcg accaggttgt attctgcaaa cagcctctga
     1501 gatacgaaaa attgtggtta aagtcgcatt aacagcgctg aattaatcga ataatcgtct
     1561 acgccagaac gcctgggtaa tgttattgct ctaactatat taataattta ggttgctgca
     1621 catgcaactt gaattatgac agacatagcc tcaaggaata gctatgaaaa aaatacttga
     1681 agcaatactg gcgatt
//
LOCUS       DACBVI010000293         1662 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.297, whole genome shotgun sequence.
ACCESSION   DACBVI010000293 DACBVI010000000
VERSION     DACBVI010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.297"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            12..584
                     /locus_tag="IU325_22815"
     CDS             12..584
                     /locus_tag="IU325_22815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HAP5811807.1"
                     /translation="MKNILVISGHPELTHSVANATILDEVETALPDVEIRRLDRLYPD
                     GKFNIAAEQKSLLRADVIVWQFPFSWYGLPGLMKKWLDEVFVHGFAHGSTAKLGGKKL
                     ILSFTTGAPEELYTVDSFFGHTIEEYLIPFETTARLCNLELLAPVYTCGISYADRDAD
                     KIAQQKTLAREHASRLVELLNSVVNNPEGE"
     gene            587..1567
                     /locus_tag="IU325_22820"
     CDS             587..1567
                     /locus_tag="IU325_22820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP5811808.1"
                     /translation="MQYTRLGKSDLLVSRICMGCMGFGDPSTGQHSWTLDETASRDII
                     RYGLEKGINFYDTAIAYQNGSSERYVGRALREMAKRDDIVLATKFLPRTPAQIAGGIS
                     GKEAIAQSLDQSLKNLGMDYIDLYIYHIWDYNTPIIDVLEALHTAVTAGKVRSVGISN
                     CYAWQLAKANALAEREGLMPFVSVQSHYNLIMREDERELFGLCTEDNIAMTPYSALAS
                     GRLSRKEGYTRRATEDDYARGKYDSTAEQDRSIIERVAELAERHLVSMTEISLAWLLT
                     KVTSPVVGATKKAHVDGAVNAVNLQLSPEEIQFLEETYQPHVLTGVMAQN"
BASE COUNT          426 a          421 c          435 g          380 t
ORIGIN      
        1 aactgattga aatgaaaaat atccttgtta tttcaggtca tcctgagctt acccattccg
       61 tcgccaatgc cactattctt gatgaagtgg agaccgccct tcccgatgtt gagattcgtc
      121 gtctggacag gctctatccg gatggcaaat tcaacatcgc ggcggagcag aaaagcctgc
      181 tcagggccga tgtgattgtc tggcagttcc ctttttcctg gtatgggctg ccaggattaa
      241 tgaaaaaatg gctggacgag gtctttgttc acggcttcgc gcatggctca acggcgaaac
      301 tgggcggtaa aaagctgatc ctctccttta caacgggagc gccagaggag ctctataccg
      361 tcgacagttt ctttggccat accattgaag agtatcttat tccgttcgaa accacggcaa
      421 ggctgtgcaa tcttgagctg ctggcgccgg tttatacctg cggtatcagc tatgccgacc
      481 gggatgccga caaaatcgcg cagcaaaaaa cgcttgcccg ggaacacgct tcaaggcttg
      541 tcgaactgct caattccgtc gtgaataatc cagagggaga ataacaatgc agtatacccg
      601 gctcggtaaa agtgacttac ttgtttcccg catctgcatg ggatgcatgg ggtttggcga
      661 tccgtcgacg ggtcagcata gctggacgct ggacgaaaca gcaagccgcg acatcattcg
      721 ctatggcctt gaaaagggca tcaactttta cgataccgct atagcctacc agaatggctc
      781 cagtgagcga tacgttggcc gggcattacg ggagatggcg aaacgcgatg atatcgtcct
      841 ggccaccaag tttctgccgc gaacccccgc acaaattgcc ggagggatca gcggaaaaga
      901 ggcaatcgct caatcccttg accagagcct gaagaatctg gggatggact atatcgacct
      961 ctacatctac cacatctggg attacaacac gcccattatc gacgtgcttg aagcgttaca
     1021 taccgctgtt actgctggca aagtacgatc tgttggtatt tcaaactgct atgcctggca
     1081 gctggcgaag gcgaacgccc ttgctgaacg cgaagggctg atgccctttg tttccgtaca
     1141 gagccactac aatctaatta tgcgcgaaga tgaacgggag ctcttcggcc tgtgcactga
     1201 ggataatatc gcaatgaccc cctatagcgc gctggccagc ggccgcctct ctcgtaaaga
     1261 aggctataca cggcgagcca cagaggatga ctatgcgcgt ggaaaatatg acagcacggc
     1321 tgaacaggat cgcagcatta ttgagcgtgt agccgaactg gccgaacgcc atctggtgtc
     1381 aatgacggaa atatctctcg cttggctgct gacaaaagtc acatcacctg tcgtaggggc
     1441 tacaaaaaaa gctcatgtcg atggcgcggt aaacgcagta aatcttcaac taagtccgga
     1501 ggaaattcag tttctggaag aaacctacca gccgcatgtt ctgacggggg tcatggcgca
     1561 aaattaaggt ggctatacgt catctgaaat tgaaacaggg tgtgtgtggc atggctacgc
     1621 acacccgagc aattcaccaa tgttcatctc attattaatg ag
//
LOCUS       DACBVI010000294         1644 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.298, whole genome shotgun sequence.
ACCESSION   DACBVI010000294 DACBVI010000000
VERSION     DACBVI010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.298"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..>1644
                     /locus_tag="IU325_22825"
     rRNA            <1..>1644
                     /locus_tag="IU325_22825"
                     /product="23S ribosomal RNA"
BASE COUNT          414 a          365 c          519 g          346 t
ORIGIN      
        1 gaatgctgac ataagtaacg ataaagcggg tgaaaagccc gctcgccgga agaccaaggg
       61 ttcctgtcca acgttaatcg gggcagggtg agtcgacccc taaggcgagg ccgaaaggcg
      121 tagtcgatgg gaaacaggtt aatattcctg tacttggtgt tactgcgaag gggggacgga
      181 gaaggctatg ttggccgggc gacggttgtc ccggtttaag cgtgtaggct ggttttccag
      241 gcaaatccgg aaaatcaagg ctgaggcgtg atgacgaggc actacggtgc tgaagcaaca
      301 aatgccctgc ttccaggaaa agcctctaag catcaggtaa catcaaatcg taccccaaac
      361 cgacacaggt ggtcaggtag agaataccaa ggcgcttgag agaactcggg tgaaggaact
      421 aggcaaaatg gtgccgtaac ttcgggagaa ggcacgctga tatgtaggtg aagcgacttg
      481 ctcgtggagc tgaaatcagt cgaagatacc agctggctgc aactgtttat taaaaacaca
      541 gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt gccggaaggt
      601 taattgatgg ggttagcgca agcgaagctc ttgatcgaag ccccggtaaa cggcggccgt
      661 aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac ctgcacgaat
      721 ggcgtaatga tggccaggct gtctccaccc gagactcagt gaaattgaac tcgctgtgaa
      781 gatgcagtgt acccgcggca agacggaaag accccgtgaa cctttactat agcttgacac
      841 tgaacattga gccttgatgt gtaggatagg tgggaggctt tgaagtgtgg acgccagtct
      901 gcatggagcc gaccttgaaa taccaccctt taatgtttga tgttctaacg ttggcccgtt
      961 atccgggttg cggacagtgt ctggtgggta gtttgactgg ggcggtctcc tcctaaagag
     1021 taacggagga gcacgaaggt tggctaatcc tggtcggaca tcaggaggtt agtgcaatgg
     1081 cataagccag cttgactgcg agcgtgacgg cgcgagcagg tgcgaaagca ggtcatagtg
     1141 atccggtggt tctgaatgga agggccatcg ctcaacggat aaaaggtact ccggggataa
     1201 caggctgata ccgcccaaga gttcatatcg acggcggtgt ttggcacctc gatgtcggct
     1261 catcacatcc tggggctgaa gtaggtccca agggtatggc tgttcgccat ttaaagtggt
     1321 acgcgagctg ggtttagaac gtcgtgagac agttcggtcc ctatctgccg tgggcgctgg
     1381 agaactgagg ggggctgctc ctagtacgag aggaccggag tggacgcatc actggtgttc
     1441 gggttgtcat gccaatggca ctgcccggta gctaaatgcg gaagagataa gtgctgaaag
     1501 catctaagca cgaaacttgc cccgagatga gttctccctg actccctgag agtcctgaag
     1561 gaacgttgaa gacgacgacg ttgataggcc gggtgtgtaa gcgcagcgat gcgttgagct
     1621 aaccggtact aatgaaccgt gagg
//
LOCUS       DACBVI010000295         1627 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.299, whole genome shotgun sequence.
ACCESSION   DACBVI010000295 DACBVI010000000
VERSION     DACBVI010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.299"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..1300)
                     /locus_tag="IU325_22830"
     CDS             complement(<1..1300)
                     /locus_tag="IU325_22830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HAP5811809.1"
                     /translation="MVDNVTVGSVCAQSPSFMPELDGGKNKTQLIVDDIVAYLKNPSV
                     YSLEKEGPLNYFFNNCSEIEPWVLIGGGLIIIVSTSEQDPESLIVTVGDIEGKYATQI
                     PVPPSLTTSLKDIFIRPHTNADDESFTKEQKKANSKYDIVDYNCKVYFNEKPEDLVVC
                     RHISLQYCIDSMNENTGKVPLKECYSTPEGIQQHFPYELDQQFDNLINNPPPGTCVVA
                     SDKFGEILSVFFHRMEKEKLTHMAAIVKSQKHAMAVRLRIKQTPAGETEYVVSFYDPN
                     ATNTAVRYKAKNCDSFGSLQSFINIELANIKWVKTEICSECVGIIPYLPREQAHLLSG
                     IDNELQPPLSPSALYLLMQMGTYENIVLFFDKLRNSQEMTVSKVLEILAAKGPGGTYG
                     LCMSLQHDNLNKINEYIKNLKELARKFNFSREDLETLLLAK"
BASE COUNT          494 a          322 c          260 g          551 t
ORIGIN      
        1 ctttcgcaag gagtagagtt tccaggtctt cccggctaaa gttaaatttt ctggccaact
       61 cttttaaatt ttttatatat tcattaatct tattaagatt atcgtgttgc agtgacatac
      121 ataacccata tgttccccct ggtcctttag cagcaagaat ctcaagaacc tttgatactg
      181 tcatttcttg ggaatttctt aacttatcga aaaaaagaac aatattttca tacgtcccca
      241 tttgcattaa tagatataat gctgatggtg ataatggtgg ttgcaattca ttgtctatac
      301 cacttaataa atgggcctgc tctcgaggga gataaggaat tatgcctaca cactcggagc
      361 aaatctctgt ttttacccat tttatattcg ccaactcaat atttataaat gattgtaatg
      421 acccaaaaga atcacagttt tttgctttat agcgtactgc agtattggtt gcattaggat
      481 cataaaagga tacaacatat tcagtttctc cggcaggcgt ttgttttatc ctcaagcgga
      541 cggccatagc atgtttttga gatttaacga ttgccgccat atgcgttaac ttctcttttt
      601 ccattctgtg aaagaagaca gataatatct ccccgaactt gtcactggca acgacgcatg
      661 tacctggtgg tggattgttt attagattat caaattgctg atcaagttcg tatggaaaat
      721 gttgctgtat cccttctgga gttgaatagc attcctttaa tggaacttta cctgtatttt
      781 cattcattga atctatacaa tactgcagcg aaatatgcct gcacacgact aaatcttctg
      841 gtttttcatt aaaataaacc ttacagttat aatctacaat atcatattta ctattagctt
      901 ttttctgctc tttagtaaaa ctctcatcat ctgcgttcgt atgaggacga ataaaaatat
      961 ctttgagtga tgttgtaagc gagggtggta cgggaatctg tgtagcatac tttccttcaa
     1021 tatcccccac agttacaatc agactttcag ggtcttgctc agatgtggag acaataataa
     1081 taagtccacc accaattaaa acccatggtt caatctcgct acaattatta aaaaaataat
     1141 ttaacggacc ctctttttca agtgaataaa ctgaaggatt tttaagatac gccactatat
     1201 cgtcaacgat taattgagtt ttattttttc ctccatccaa ctcaggcatg aaagaaggag
     1261 attgtgcaca tacactgcca acagttacgt tatcaaccat tttctttccc ctcattcata
     1321 tgaaagttgt agtaataata aggttactat ttaaaaacca actatcaaat ttcgctcatt
     1381 tatagagtta tttgtctttt tacgtcatta ccccatcagg tcatcaacct ggtaatgaag
     1441 tgagtaaacc gcgacgtcaa cgatggaaac cgctccttgt aatggaaccc ctataatcaa
     1501 tgccatcacc ttcaacaata atgataaaaa ttaaaaaatt tatccattca tgtgtttttg
     1561 ctaatatttt gcatggggca gctatcacaa acacatccaa tatctaattg tgattcgatt
     1621 cacattt
//
LOCUS       DACBVI010000296         1607 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.300, whole genome shotgun sequence.
ACCESSION   DACBVI010000296 DACBVI010000000
VERSION     DACBVI010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.300"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(519..1592)
                     /gene="ychF"
                     /locus_tag="IU325_22835"
     CDS             complement(519..1592)
                     /gene="ychF"
                     /locus_tag="IU325_22835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002422463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP5811810.1"
                     /translation="MVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVVPMPDPR
                     LDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGHVVRCFE
                     NDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKAELAVLE
                     KCLPQLENAGMLRALDLSAEEKTAIRYLSFLTLKPTMYIANVNEDGFENNPYLDQVRE
                     IAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKLLNLQTY
                     FTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQGAKEAG
                     KMRAEGKDYIVKDGDVMNFLFNV"
BASE COUNT          390 a          380 c          366 g          471 t
ORIGIN      
        1 tatgggattt tcgcttgatg tatctgcata acaaaaacaa tatgagccat ttttgcatta
       61 ctaataagaa aaagcaaaaa acatcgatat tcatttttga tcaatggatt ttctggcata
      121 tcgttattat cgtgatactg gttatcattt tcatcttgcc aggttcgcac gtaatcacgt
      181 tattcgggga ttttatctgc gggatattac ccttcaggta agtccttttc tcgccagcat
      241 attggagcgt gatcaatatt gatcagctgt aaaaaggtgt ctgatgtgca gtggaacaca
      301 aacttacgtt atctcattgc taatgagata tccctcatct gaacacttat gtctttaatt
      361 attctcgtgg ttcactatag gcaataagca caaaagtgta ggatgttata agaatggtta
      421 ggactcggtg aaatgaaaaa tccacgcaat tgcgtggatt ttatatactt ttgcgctctt
      481 catgagattt agcgaaacct catgagacaa caaattaatt agacgttgaa caggaagttc
      541 attacatcgc catctttaac gatgtaatct ttgccttctg cacgcatttt gcctgcttct
      601 ttcgcgcctt gttcaccttt gtaagtgatg aaatcttcaa acgagatggt ttgtgcacgg
      661 ataaagcctt tttcaaaatc agtatggatt ttgccagcgg cctgcggcgc ggttgcacca
      721 accggaatgg tccatgcacg tacttctttc accccagcgg tgaagtaagt ttgcaggttc
      781 agcagcttat aaccggcacg gatcacacgg ttcaggcccg gctcttccag cccaagctcc
      841 tgcataaact cgtcacgttc ttcgtcgtcc agttcggcaa tgtctgcttc aacagcagca
      901 caaaccggaa ccacaacaga accttctttc gccgcgattt cacgcacctg gtcaagatac
      961 gggttgtttt caaaaccgtc ttcgttgacg ttggcgatgt acattgttgg tttcagcgtc
     1021 aggaagctca ggtaacgaat agccgttttc tcttcagcgc ttaaatccag cgcgcgcagc
     1081 atacctgcgt tttccagctg cggcaggcat ttttccagta ccgccagctc ggctttcgcg
     1141 tctttatcgc cacctttggc tttcttctgt acgcgatgaa tcgcacgttc gcaggtgtcg
     1201 aggtccgcca gcgccagttc ggtgttgata acttcaatat catcagccgg gttaactttg
     1261 ccggaaacgt gaatgatgtt gtcattttca aagcagcgaa caacgtgacc gatcgcttca
     1321 gtttcacgga tgttggtcag gaactggtta cccagacctt cgcctttcga tgcgcctttt
     1381 accagaccgg cgatatcgac aaattccatg gtcgtgggaa gcgtacgctg cggttttacg
     1441 atttcagcca gttgatccag gcgagggtca ggcattggta cgacgcctgt gttcggctca
     1501 atggtgcaga atggaaagtt ggccgcttca ataccggctt tggtcagcgc gttgaacagg
     1561 gtagatttcc cgacgttggg caaaccgacg ataccgcatt tgaatcc
//
LOCUS       DACBVI010000297         1596 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.301, whole genome shotgun sequence.
ACCESSION   DACBVI010000297 DACBVI010000000
VERSION     DACBVI010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.301"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            112..699
                     /locus_tag="IU325_22840"
     CDS             112..699
                     /locus_tag="IU325_22840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811811.1"
                     /translation="MKGHNFLPLAVSLVFLSGCQQLNESVNSVNGAINSSLSSLNSTV
                     DKALGVSASGQDSGPATSITGLGAVCKDYEENAMAAEKKWTGKRIAITNATVLEVTKG
                     RSASDMMTGMPKSAPNYYLLFKTADTSYCGGMVWLDYYSGIDDAVLTYKKGQKVSVTG
                     TVNNFEVRGFATVNNADMPVRLVVLRNGTINHKKG"
     gene            705..1235
                     /locus_tag="IU325_22845"
     CDS             705..1235
                     /locus_tag="IU325_22845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811812.1"
                     /translation="MRKFLAIAGLAVLLSGCGEKGDFEKAINAKIGQSKTCFSLRNND
                     VTFPIQLSKPRFDETGTGTGSVILDGLVEQGLMAFVKGWDNNTLGITDEGKQAKIWDA
                     KEGVCIGRQVVDEVKEWTEPGNGNQKVVRVSYTWKLADVPSWVDKSAFSGVKGMNESA
                     DGAMNLVKTSNGWKAN"
     gene            1245..1520
                     /locus_tag="IU325_22850"
     CDS             1245..1520
                     /locus_tag="IU325_22850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811813.1"
                     /translation="MENNTIRCPFCLKESQRGVHVCTGCMATVLYGAYPGWYAFVVIL
                     LTFGLSILIGMSTGMAGATISLPIIAAVGFVAGKAIFSDNVVFRRRM"
BASE COUNT          466 a          319 c          394 g          417 t
ORIGIN      
        1 gaacactaaa aataattcaa ttctatcaat tgattagacg atagctcctg atctataggt
       61 cagtgcgccg ggattggggt atacgaatct gactaaatcc ttgaggaata aatgaaagga
      121 cataacttct taccactggc ggtctctctg gtgtttcttt ctggctgcca gcagctcaac
      181 gaaagcgtga atagcgttaa tggggcaatt aactcttcac tgtcatcatt gaacagcacc
      241 gttgataaag cgctgggggt ttcagcttcc ggtcaagata gtggtccagc gacgtccatt
      301 actggccttg gtgctgtctg caaagactat gaagaaaatg cgatggcagc agaaaaaaaa
      361 tggacaggca aaaggatcgc tatcaccaat gccactgttc ttgaagtaac aaagggcaga
      421 agtgccagcg acatgatgac gggaatgccc aaatcggccc ctaactatta tctgctcttc
      481 aaaacagcag ataccagcta ctgcggtgga atggtatggc tggattacta ctctggtatt
      541 gatgatgcag ttcttaccta taagaaaggc cagaaagtca gcgtcaccgg aacggtaaac
      601 aatttcgaag tccgtggatt cgctaccgtc aacaatgccg atatgccagt tcgtctggtt
      661 gttctgagaa acggcacgat caatcataaa aagggataag aaccatgcgt aaatttttag
      721 ccatcgcggg tcttgccgtg ttgttatcag gctgcggtga aaaaggggat tttgagaaag
      781 cgatcaacgc caagattggt caatcgaaaa cctgtttctc actacgaaac aacgatgtca
      841 cattcccaat ccagctctct aaaccgcgct tcgatgaaac aggcactgga acagggtctg
      901 tgatcctcga cggcctcgta gaacagggct taatggcttt tgtaaaaggc tgggataaca
      961 atacgcttgg gataacagat gaaggtaaac aagccaaaat ctgggatgcc aaagaaggtg
     1021 tttgtattgg tcgccaagtt gttgatgaag tcaaagaatg gactgaaccg ggcaacggta
     1081 atcagaaagt tgttcgcgta tcttatacat ggaagctggc agatgtccct tcatgggtgg
     1141 acaaaagcgc gttctctgga gtgaaaggga tgaatgaatc tgctgatggt gccatgaatc
     1201 ttgttaaaac cagcaatggt tggaaagcga actaggggac tattatggaa aacaatacca
     1261 tcaggtgtcc attttgctta aaggaaagcc agagaggtgt tcatgtctgc accggttgca
     1321 tggcgactgt tttatatgga gcctaccccg gctggtatgc tttcgtggtt atattattaa
     1381 ccttcggcct gtctatactg atcggaatgt ctacaggcat ggcgggagct actatcagcc
     1441 taccaatcat tgcggctgta gggtttgtcg caggtaaggc aattttctct gataatgttg
     1501 ttttcaggag aaggatgtaa atatcgtttt taataaatac gccacatttt aatcaaagat
     1561 gtggctttca taaaaatcga tttaatttac ataatt
//
LOCUS       DACBVI010000298         1575 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.302, whole genome shotgun sequence.
ACCESSION   DACBVI010000298 DACBVI010000000
VERSION     DACBVI010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.302"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..570)
                     /locus_tag="IU325_22855"
     CDS             complement(<1..570)
                     /locus_tag="IU325_22855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811814.1"
                     /translation="MIFTELKSNGQPIYYGKYYQQVCNIVGDSSLIHDHNNKFMRSYF
                     QQINRISEHYLNHLDVIIKEFELPSTWQPKGVYKLIVTTTMLGGKYHVDDTYVADKYA
                     LSSFFQRIPGVIYQTNENGKMAKNIIDGFECCEGEITIDKFIDYLSSLPSINAVRKNI
                     KKLTYSVRFNEKLVHHPYYDSWTFGPYIRK"
BASE COUNT          527 a          288 c          243 g          517 t
ORIGIN      
        1 cttacgaata tatgggccaa aagtccaaga atcataataa gggtgatgga ctaatttttc
       61 attaaatcga acactataag ttaatttttt tatattttta cgtacagcat taatactggg
      121 caaagaggaa agataatcta taaacttatc gatagtgatt tccccttcgc aacactcaaa
      181 cccatcaatg atatttttcg ccatttttcc attttcattt gtttgatata tcactccagg
      241 tattcgttga aaaaaactag ataatgcata tttgtccgct acatatgtat catccacatg
      301 atatttccca ccgagcatag ttgtagtaac aataagctta tacacacctt tgggttgcca
      361 tgttgatggt agttcgaatt ctttaatgat cacatcaaga tgattaagat aatgttcaga
      421 aattcgattt atttgttgaa aataagaacg cataaattta ttattatgat catgtattaa
      481 tgagctatct ccaacgatgt tacacacttg ctgatagtac ttaccataat atattggttg
      541 accatttgat tttagttctg taaaaatcaa acaatcatcc aggagaatca cgcaatcgca
      601 ctgataatta gcatgagcat caattggaga agctttaatc cccgcatcac gaacttgttt
      661 tataaattgt tgctcatgaa atcacccttt acttgcttga gaaatttttt tggaaccaaa
      721 taatgacatt agagagcgag tcactgagct atttgctatt aatgcaggca ttaagcataa
      781 accgtccata cagggaataa atgggcaatc attaagatca tgtgattttg aattgaaggc
      841 aaaataatct atcagttttt ttgctgcatc tgctgcaatc cccttacgtt gaaagaatga
      901 taaccaactc tcacggcttt ttacgatcag ccagcgatcc aactgtaggg gtataggttt
      961 tgtcgatgaa aatcttttgc tcatttcttc tttagataac tcgactaaac attgataagc
     1021 gtgcacccaa ttaaataatg atatattccc atattcttca tgaaggtcat caggagacat
     1081 attttccaac tcgcttagat gaaatgagct aaaaacgcca tcagaattta agtaagatgg
     1141 catatgatcc tttacccaac tatttagatg aaaatgtttg ttactaaatt catgaacttc
     1201 gctatataga tgtgacgctc tcatcattag tctatttctt gcaatctcat aatctcggta
     1261 taaacaaaaa ctatcaacct caagagagta aatgttatta tccaattttg taaatactgc
     1321 actacttaat ctgaaaaagt ctcttactct gcttaatgag tcaaaaacat tccactcatt
     1381 aaggtaccct cccattgaaa gcaaggtttg tttattcaca caagaagtta gtttcaatgg
     1441 cccatgagtc cgtaaataag aaaatattct tccggtgtaa ttggctacct catcaaatgc
     1501 cctatcgata actcgaaaat attcgatatc aggttcagca tgaatattaa tattttcaga
     1561 aacgataaat aatgg
//
LOCUS       DACBVI010000299         1561 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.303, whole genome shotgun sequence.
ACCESSION   DACBVI010000299 DACBVI010000000
VERSION     DACBVI010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.303"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..302)
                     /locus_tag="IU325_22860"
     CDS             complement(<1..302)
                     /locus_tag="IU325_22860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Required for surface presentation of invasion
                     plasmid antigens; required for invasion and for secretion
                     of the three ipa proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HAP5811815.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTVSVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGIGWLKTIIPFLLVCMFTTLLVSWFQS"
     gene            complement(311..1078)
                     /locus_tag="IU325_22865"
     CDS             complement(311..1078)
                     /locus_tag="IU325_22865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscT/YscT/HrcT family type III secretion system
                     export apparatus protein"
                     /protein_id="HAP5811816.1"
                     /translation="MGEAMLYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELSTLNEIDIALFAAREIIIGLFIACLLASPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTNGGLNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLARYASQLNAFAISLTV
                     KSALAFLILIIYFGPILAERVMPLSFFPEQLQLYIDK"
     gene            complement(1080..1340)
                     /locus_tag="IU325_22870"
     CDS             complement(1080..1340)
                     /locus_tag="IU325_22870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HAP5811817.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(1350..>1561)
                     /locus_tag="IU325_22875"
     CDS             complement(1350..>1561)
                     /locus_tag="IU325_22875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HAP5811818.1"
                     /translation="AFIIGFYIYLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLIL
                     FVAMDGWTMLSKGLILQYFDLSINP"
BASE COUNT          551 a          320 c          267 g          423 t
ORIGIN      
        1 ttactttgaa accatgaaac aagtagtgtt gtaaacatac aaaccagcaa aaaaggaatg
       61 atcgttttca accaacctat ccccatagct ttaaaatagt cccaaatatc cggtttagcg
      121 ttatgatcaa ggatgtattc aagaatcgac ataatgtgat gcacatcaaa tacatatcca
      181 agatataaag tacccacaag cattattaca gagactgtta agtctctact ttttagaatt
      241 tgaccttttt tagaagcatc ctggagtttc ttttgtgttg gcttttcagt tttatttgcc
      301 atgaaacact ctatttatca atgtataatt gcaattgttc tgggaaaaag gataacggca
      361 taactcgttc agccagtatt gggccaaaat aaataattaa tattaaaaat gccagcgcac
      421 ttttgacagt cagtgagatg gcaaaagcat ttagttgtga agcataacgt gccagcaatc
      481 ccaggacagc ttcaccgccc agcataaccg caatcacggg gcttgcgtaa acgatagtat
      541 gagtcattat attattaata tatgagaata aaggctctaa tttaggaaaa ttaaaatttc
      601 ctgaaggcca taagttataa ctttgccata gagtctccag aataaagttc aatccaccgt
      661 tagtaagata aactgctgca gagaaaaggt taaataatct tgctaattcc gatgtatcga
      721 caccagtagc cggatccaaa gtactactta acgttgcacc acgttggtta tcgataaagc
      781 tgcctatagc aaggaaaatc caaaatgggg atgctaataa gcaagcaata aacaacccta
      841 tgataatttc tctggcagca aacaaagcga tatctatttc atttagggta gataattcat
      901 agtggtaatg ctgaactaat gcacatgata ccactattat tattggatga cggactactg
      961 ttggtatatt cccactggcg aaaaatggta acaaatagaa agttggtgcc aatcggcaaa
     1021 aaccgagcgc tgtagcagcc agcagactat gcaactgata gagcatcgcc tcccccatgc
     1081 tatcctctcg cgaatgctaa attcagcatt tcgataccaa acgaatataa tttttcacca
     1141 taccatccgc tcattaaaaa aaagcaaata cttacgcaca ataatttgac gccaaatggc
     1201 aacgtctgtt cttgcaactg tgttaccgtt tgaaaaagcc ctaccaataa accaacaaat
     1261 gtcgcaactg caataggtcc tgcagacata actaaaatca gatacagcgc cctattcccc
     1321 gcaaaaacaa tgtcgtccat attttttcct cagggattaa tggataggtc aaaatattgt
     1381 aaaatgagtc ctttcgataa cattgtccaa ccgtccatcg cgacaaacag aattaatttt
     1441 atcggtgtag atatggttac cggactcatc atcatcatac ctaacgttaa cagaacgctg
     1501 gatataacca gatcaactac aacaaagggt aaataaatat aaaaacctat aataaatgca
     1561 c
//
LOCUS       DACBVI010000300         1552 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.304, whole genome shotgun sequence.
ACCESSION   DACBVI010000300 DACBVI010000000
VERSION     DACBVI010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.304"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(33..1451)
                     /locus_tag="IU325_22880"
     CDS             complement(33..1451)
                     /locus_tag="IU325_22880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP5811819.1"
                     /translation="MVTINTESALTPRPLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT          437 a          437 c          358 g          320 t
ORIGIN      
        1 ggccggatgt acagcacatc cggcccgtga aatcagacgc cgatatttct caacttctcg
       61 cctgccatca gtttgcgttc gatatgttcc agggtgacat ttttggtttc cggaatgagc
      121 cagaaagtga tgcccacaaa cgcaatgttc agcgcagtgt agagccagaa tgtaccggca
      181 gcgccaatgc tatcaagcag tgtcaggaag gtcgcgccga taatcatatt cgacacccag
      241 tttgtcgtcg ttgagcaggt gatgccaaaa tcgcggcatt tcagcggctg aatttcagag
      301 cacaagatcc acaccactgg cgcggcgctc atcgcataac cggcaataca catcatcgtc
      361 atgccaacag agagccagga caagccactg gaagccgtac cgttatcaaa ctgcatcagg
      421 cagtaaccca gcaccagagt gcctaacgcc atcacgctga aaccaatttt cagagccggc
      481 ttacgccctg ctttatctac cgtaaagacc gcaatgaagg tggcgaacat aaaggtcagc
      541 cccacgacca gagtcgcaat catctgttgt tctgtggtcg taaagcccgc cattttgaag
      601 atacgcggcg cgtagtacat gatgatgttc ataccggtaa actgctgcat cgcctgcaac
      661 aacataccga gaaatacagc acgacggacg ttacggttga tcttaaacag tgcccaaccg
      721 ccctgtttta acttcaggct ttcacgaatt tcgttgagtt cttctcgcgc tttttccgac
      781 gtatcgcgca gcatacgcaa tacttcttcc gcctcaatat gacgcccttt ttccgccagc
      841 cagcgcgggc tatttggcag gaagacaacc agaataatca gcaggactgc tggtaaagca
      901 agaaccccca acattgcgcg ccagttaccg ctataactga acgctgtatc ggataaaaac
      961 gccagcacga tgccgagtgt gaccatcaac tggtacatac tgatcatctt accgcgaacg
     1021 ttttcactgg ccatttcaga aagatacaga ggagcggtgt aagacgcgat cccgacagca
     1081 atgcccagca ccacacgagc ggcgattaac atctctacgc tggtcgcaaa agcggaccct
     1141 atcgagccga gcacaaacag gatggccccc gccatcaggc tgtatttacg ccccaggcgg
     1201 aacgacagcc aaccattaaa cagcgcacca attgccgcgc cgagcatcat gctactgacc
     1261 acccattcct gcaaacggct ggtcagcaca aagtgatcgg taatgaacgg caacgctccg
     1321 gcgattacgc cgatatcaag accaaataac aatcctgcga ccgcagcagc taccgaaaca
     1381 aacatattca tacgccgcgt atcccgcaga ggacgtggcg ttaaagcaga ttccgtattg
     1441 atagtaacca ttttttcctg ccagcagaga gtaagacata gtgaaaaaat acgtgaacaa
     1501 ttcacacagg tgtcaggtcg gaaatagcat aaatatggat taaattgcta cg
//
LOCUS       DACBVI010000301         1538 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.305, whole genome shotgun sequence.
ACCESSION   DACBVI010000301 DACBVI010000000
VERSION     DACBVI010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.305"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..1060
                     /locus_tag="IU325_22885"
     CDS             <1..1060
                     /locus_tag="IU325_22885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HAP5811820.1"
                     /translation="AANVAHAAEVYNKDGNKLDLYGKVTALRYFTDDKRDDGDKTYAR
                     LGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAGLKISEFGSLDYGRN
                     YGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGVATYRNYDFFGLIEGLNFAAQYQ
                     GKNERTDNGHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKSDRTNAQERAAANPL
                     NASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNKAQSFEVVAQYQFDF
                     GLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYVDYKINLIDESDFTR
                     AVDIRTDNIVATGITYQF"
     gene            1224..>1538
                     /locus_tag="IU325_22890"
     CDS             1224..>1538
                     /locus_tag="IU325_22890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAP5811821.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLAQWGNKS
                     LNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDFT
                     DNA"
BASE COUNT          451 a          326 c          325 g          436 t
ORIGIN      
        1 agctgcgaac gtagcacacg ctgccgaagt ctataacaag gatggtaata aactagacct
       61 ttatggcaag gttaccgctc tacgttattt tactgatgat aagcgtgacg atggtgataa
      121 aacttatgcc cgtctcggct ttaaaggaga aacgcaaatc aatgatcaaa tgattggttt
      181 tggtcactgg gaatatgatt ttaaaggcta taacgatgaa gccaacggct cgcgcggtaa
      241 caagacccgt ctggcctatg caggtttaaa aattagtgaa tttggctctc tggactatgg
      301 tcgtaactac ggtgtcggct atgacattgg ttcatggacc gatatgttgc cagaatttgg
      361 tggcgatacc tggagtcaga aagatgtctt catgacatac cgtactaccg gtgtggcaac
      421 ctatcgcaac tacgatttct tcggcttaat tgaaggtctg aactttgccg cgcaatatca
      481 aggcaaaaat gaacgcactg acaatggtca tctttatggt gctgactaca cgcgtgccaa
      541 tggtgacggt ttcggtatct cctcaactta tgtttatgat ggctttggta tcggtgcggt
      601 gtataccaaa tccgatcgga caaatgcgca ggaaagagcc gctgctaatc ctctcaatgc
      661 ctccggtaag aatgcagaac tgtgggctac aggtataaaa tatgatgcca acaacatcta
      721 ctttgcagct aattacgctg aaacattaaa catgaccacc tatggcgatg gttatatctc
      781 taacaaagca caaagttttg aagtagtggc acaatatcaa ttcgacttcg gcttgcgccc
      841 atcactcgct tacctgaaat cgaaaggcag agatctgggc cgctacggcg accaggacat
      901 gattgagtat atcgacgttg gtgcgacgta tttcttcaac aaaaatatgt cgacctatgt
      961 tgattataaa atcaacctga ttgatgaaag cgactttacc cgtgccgtag atattcgcac
     1021 cgataacatc gtcgcaacgg gaattaccta tcagttctaa catcctctcc tgaccagccc
     1081 ttctctgggc tggtttttga tttaagtgct gctgtatgac tccttaagta aaaatgagca
     1141 atttttgata tttctggtca ttcacctcaa ttaatattct tccataccct ccggggacat
     1201 cttactataa tatttattga tgagtgatta tgaccaatat taatacagct tgtgtaaaaa
     1261 acaatgccag ttatcaatta aataacgcat tacctaacaa agaaaccatc tccagcaatt
     1321 tttgtgaacg actggcacaa tggggtaata agtcgcttaa taatggtgaa gaaagagcaa
     1381 ttgccgtaga gcgaattaaa gaagcttaca attcgaacat ggcatctctt gatttgtcat
     1441 atcttgattt gagtgagtta ccgcctatcc cctcgacagt taacacgtta aatctggaaa
     1501 acaattgtct cacttgtctt gactttactg acaatgcc
//
LOCUS       DACBVI010000302         1487 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.306, whole genome shotgun sequence.
ACCESSION   DACBVI010000302 DACBVI010000000
VERSION     DACBVI010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.306"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..1381
                     /locus_tag="IU325_22895"
     CDS             <1..1381
                     /locus_tag="IU325_22895"
                     /inference="COORDINATES: protein motif:HMM:NF017011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811822.1"
                     /translation="YHSDVIASNGNYTNFFEALLLNKKVFFVKYAEFMRGWEDTDISV
                     VSNYCELEALMDVFLAENGGLLQGDKELSARMFSVRKFDSLATNRIVDTLSEFVTSTS
                     TNNLSRMPSAKERFLNKESIDVVAIPSDEKTLCNTQLYLGDLLNSNTLLSTFDKDITL
                     SKLAAVDVFCQWGIKPNSTKRIQREKAKKLGRPVFILEDGFIRSVKIGLSSAPGLSVI
                     IDDSTAYYDATKQSRLERLLQEGPELNNEQKKRARNAINKIVSNRVSKYNDSINVHLP
                     VGRNGVDKVLLIDQRNGDMSVPSALADDSSFELMLANAISNNPDADIIIKQHPDAIKG
                     GKSSYFNNDLIEKYRKISPNIYTVNMDINPYAMFEYVKEVYVVSSGVGFEALMAGKKV
                     YCFGMPFYAGWGLTQDQLYLERRSRKRTLEDIFYFAYIECSRYYNPDEKRVVEVEDIV
                     DFIVKHRKV"
BASE COUNT          487 a          204 c          304 g          492 t
ORIGIN      
        1 atatcatagt gatgtgattg catcaaatgg aaattacaca aacttttttg aagctctgtt
       61 attgaataaa aaagttttct tcgtaaaata tgctgaattt atgagggggt gggaagatac
      121 cgatatttca gttgtgagta attattgtga acttgaagca ttaatggatg tttttcttgc
      181 ggaaaatggg gggttattac aaggggataa agaactatca gctagaatgt ttagtgttcg
      241 taagtttgac agtctggcaa caaaccgtat tgtagataca ctttctgaat tcgttacatc
      301 tacgtcaact aataatttat cgagaatgcc ttcagcaaaa gagcgttttc ttaataaaga
      361 aagtatagat gtggttgcaa ttcctagtga tgaaaaaacg ctttgtaata cccaacttta
      421 tttgggtgat ttattgaatt caaatacact tttaagcact tttgataaag atataacttt
      481 atcgaaatta gcggcagttg atgttttctg ccagtgggga ataaaaccaa attccacaaa
      541 gagaattcaa agagaaaaag ccaaaaaact tggtcgacct gtttttattt tagaagatgg
      601 gtttattcgt tcagttaaaa taggtctttc atctgctcct ggattatcag ttattataga
      661 tgattcaacg gcttattatg atgccaccaa gcaatcacga cttgaaaggt tattacagga
      721 aggtccagaa ttaaataatg agcaaaaaaa acgtgctcgt aatgcaatta ataagattgt
      781 cagtaataga gtttccaaat ataatgactc tataaatgta catttaccag ttggtcgtaa
      841 tggagttgat aaggtattgt tgattgatca acgtaacggt gatatgtctg ttccctcagc
      901 acttgcagat gatagctctt ttgagttgat gttagccaac gcgatcagta ataaccccga
      961 tgcagatata attatcaagc agcatcctga tgcaattaag ggtgggaagt ctagttattt
     1021 taataatgat ctcatcgaaa aatatcgtaa gatttcacct aatatttaca ccgtgaatat
     1081 ggatatcaat ccttatgcaa tgtttgaata tgtcaaagaa gtttatgttg tatcttcagg
     1141 ggtaggtttt gaagcattga tggcgggtaa aaaagtatat tgttttggta tgccatttta
     1201 tgcagggtgg ggcttaactc aagaccaatt atacttagag cgtcgaagtc gtaaaagaac
     1261 cttggaagat attttttatt tcgcatatat tgaatgcagt cgatattata accctgatga
     1321 gaagcgagtt gttgaagtag aggatatagt ggactttata gtgaagcatc gtaaagttta
     1381 aaaaatcatg ctcgtagtcc tgaggaattt ccactgattt tatttagttt atgcatgaaa
     1441 ctatgataag tgcagtgtga gtaagttact ttatttcttc gcaaata
//
LOCUS       DACBVI010000303         1463 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.307, whole genome shotgun sequence.
ACCESSION   DACBVI010000303 DACBVI010000000
VERSION     DACBVI010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.307"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..892)
                     /locus_tag="IU325_22900"
     CDS             complement(<1..892)
                     /locus_tag="IU325_22900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP5811823.1"
                     /translation="MDLLAESITEVTVSGKITNTDRVLNIAYGIDRNFLFGAAVSMQS
                     VIMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSEVIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVA"
     gene            complement(918..>1463)
                     /gene="rfaY"
                     /locus_tag="IU325_22905"
     CDS             complement(918..>1463)
                     /gene="rfaY"
                     /locus_tag="IU325_22905"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HAP5811824.1"
                     /translation="IDTKYGRYVFKVFAPKTKRNERFFKSFVKGDYYQNLIVETDRVR
                     SAGLTFPNDFYFLAERKFFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKL
                     HALNMLSGDPHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYG
                     YYSVIYRTKLRKFIKKLKGKA"
BASE COUNT          460 a          307 c          254 g          442 t
ORIGIN      
        1 acgccacatc attccagcaa gaagcctgat aagcctcatt aaaatacttc tggcagggat
       61 aattcgtcca gtcgttccag ggcttcgtta cgccaacata atgaatcagc ttagtatcat
      121 cggtaatggt ctttttataa tcttcatcgc ttttattttt taactcatag tcaatgccat
      181 aaaagcaatc ataatcgccg ctaataaaaa tacaatgacc aaagaacaga atatttaaaa
      241 tatcctgatc aaggcattta tacttcgtat gctgcgggtc acttgccagc atagcaatgg
      301 ctttattcat aaaatcattt ttcgcccatg cttcaagctg cagatatact acaccggcgt
      361 tgaaataatt acctggaagt ccctctatag ccagacgctt cgctggtttt tcctgcataa
      421 aaggcacatc tttaataaca gctgcgaact tatcgccatc aaaattaata tcaagcaatc
      481 cagctaatga gcctttacag atgacatcag catcgatata taacagattt tcaataactt
      541 cactcagata ctggaacgca actaaacgga agaatgttgc ataagaccac tgtttcaggc
      601 tggggaagat atcaataaag gcactggaga ctttataaat tcttacttca acgttagcat
      661 ttttgctggt aaaagcgtta acacgttgta gataatcttc atcaatgtag tcggtaaaga
      721 gatgaaactt aaccgcaagg tccgggttat gcataataac tgattgcata gatactgccg
      781 caccaaataa aaagttgcgg tcaataccgt aagcgatatt taacacacga tcggtgttag
      841 taattttccc agagacagtg acttcagtaa tactctcagc taataaatcc atgttggttc
      901 cgttttgact gtgtggttta cgctttgcct tttaattttt taataaattt gcgtagtttg
      961 gttctgtaga ttactgaata gtagccataa tcttttattt cgttggcaat acccaggtga
     1021 cgttccatgg ccagacgatc acgagcttta cgctctgccg tacagctttt accggatagg
     1081 tcaattatcc tgacaccatc tttactaacg ataaagttac cccggtgtgg gtcgcccgat
     1141 agcatattta aggcatgcaa tttttccatt gaagccttaa tttctgcttt aacattttct
     1201 ggaataatcg gcatatcatt gagctctacg ccctcgacat actcgataag catgataaaa
     1261 acactggcat agttgaaaaa tttacgctca gccagaaaat aaaaatcatt aggaaaagta
     1321 agacctgcac ttctgacacg atcggtttca acgatcaaat tctgatagta gtcacctttt
     1381 acaaaagatt taaaaaaacg ttcattcctt ttcgttttcg gagcaaagac tttaaaaaca
     1441 tagcgaccat atttcgtatc aat
//
LOCUS       DACBVI010000304         1450 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.308, whole genome shotgun sequence.
ACCESSION   DACBVI010000304 DACBVI010000000
VERSION     DACBVI010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.308"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            197..1141
                     /locus_tag="IU325_22910"
     CDS             197..1141
                     /locus_tag="IU325_22910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811825.1"
                     /translation="MSPDNIGSAVNRFSGWPSNDSLKTDKESMTSPVTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNLDEEGHYLAFG
                     YNVDTPDRITVSAYNEKKPGCYGQCLLIQDDETDLFNLIKVKIFEARHNYVAQLMSVP
                     EIMFKFSYSAQQNRLEPHLLVPTYFPLKTNEPVTQEDVLLLYRFFKMDGDFNILTSDE
                     YMSIMTPLMKCERSIHDHNRYVTGKDTLLLDYPPSGNQIYFHIFPDESATLVLYFSNN
                     DIECFVFERDIPSQYRFFKMFTNLALVIDQLTDANQIL"
     gene            1222..>1450
                     /locus_tag="IU325_22915"
     CDS             1222..>1450
                     /locus_tag="IU325_22915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811826.1"
                     /translation="MIPSQISFNTLPVNATYASETTVDTQKFSDILYSAGCSLKDLAY
                     LSRCLASIHPTIAKNIYETENLSDQKLLHIDC"
BASE COUNT          453 a          300 c          259 g          438 t
ORIGIN      
        1 cgttatgtta ttgtttcatt agtaataatc acttaaatgt atcgatgaaa caggtatgac
       61 ttagcgggag tgattgtttc tgagtgaaca gaggccactt tcgcctgata aatgaaaaat
      121 gagcgatttt tgcaagttag tttttctata gccgagtacg ctcagcgcct cgcttgatta
      181 caatgaggtt gctgctatga gtcctgataa tatcggttcg gctgtcaatc gcttctcagg
      241 ttggccatct aatgattctc taaaaacaga taaagaatca atgacatcac ccgtgactgt
      301 gcaggtaata aagatgattg ccgccatcct tgacccacat cggatagtcg ttggaatcga
      361 tacactttat acaacgcagc gcgaaacatt tacctcagag agtcaacagc tcattgaatc
      421 agcattgcaa acacttcatg tgatgaattt aaataacctg gaaacaaatc tcgatgagga
      481 aggtcactat ctggcttttg gatataacgt tgacactcct gacagaataa ccgtttctgc
      541 ttataatgaa aaaaagcctg gctgctatgg ccaatgtctt ttaatacagg atgacgaaac
      601 agacttattt aacctgataa aagttaaaat ttttgaagcc aggcataatt atgtcgctca
      661 attgatgagc gttccggaaa ttatgttcaa atttagttat agcgctcaac aaaatagatt
      721 agaacctcat cttctggttc ccacttattt ccctctaaaa acaaacgaac cggttacgca
      781 agaggatgta ctgttacttt atcgtttctt taaaatggat ggtgatttta atatattaac
      841 atcagatgaa tacatgagta tcatgacacc tctaatgaag tgtgagcgta gcatccacga
      901 ccacaacagg tatgtgacgg gcaaagatac ccttcttctg gactatcctc cttctggtaa
      961 ccagatatat ttccatattt ttcctgacga atcagcaaca ctagtccttt atttttcaaa
     1021 caatgacatt gaatgtttcg tctttgaaag agatattccg agccagtatc gctttttcaa
     1081 aatgtttacc aatctggcac tggttattga tcagttaacc gatgccaatc aaatacttta
     1141 atttttatat attccctgtg gcaaatattg ccaaacaatg cccggggatt aatttttatt
     1201 actcacggaa agatttaaat aatgatccca tctcaaatct cgttcaacac ccttcctgtc
     1261 aatgcaacat acgcttcaga gacaacagtt gacacacaaa aattctctga catcctctac
     1321 tcagcggggt gttccctaaa ggatctggct tatctgagca gatgtctggc atcaatacat
     1381 ccgactatcg ccaaaaacat ttatgaaaca gaaaatctca gcgatcaaaa attactgcat
     1441 attgattgcc
//
LOCUS       DACBVI010000305         1432 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.309, whole genome shotgun sequence.
ACCESSION   DACBVI010000305 DACBVI010000000
VERSION     DACBVI010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.309"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..>1432)
                     /locus_tag="IU325_22920"
     CDS             complement(<1..>1432)
                     /locus_tag="IU325_22920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HAP5811827.1"
                     /translation="KTIQPTVNLNGSAFFSQSPSDAIFCRHLSLQYALDSLRNGKGKV
                     NLIKHYSSVESIQQHVPLVRDAEFRSLLRHPPAGSRVIASKDFGFALDIFFCRMMANN
                     VSHMSAILYIDNHTLSVRLRIKQSAYGQLNYVVSVYDPNDTNVAVRGTHRTARGFLSL
                     DKFISSGPDAQTWADMYVRNCAIAFLPLLPEGVPGAIFAGIASRMPFAPIHPSAMLLI
                     MATGQTQQLIALFKQLPILPEKEIIEIITAQNSVGTPALFLAMMNGHTDNVKIFMQEI
                     QSLVDNHIIHEDNLVKLLQTKSANETPGLYISMLYGFDEIIDIFLNALTTPITQELLS
                     KKMVMDILAMKTRDGEPGLYAAMENNHPLCVTRFLSKVYGIAVKYNLSKINIMDLLKG
                     ATAYGTPALYIAMSKGNKDVVLSYISTLGTFANKYSFSQCQLFTLLAAKNHDNMSAVH
                     IAIHHNHYKTVETYYAAINVISQSLSF"
BASE COUNT          425 a          266 c          302 g          439 t
ORIGIN      
        1 aaaactcagg ctttggctga ttacgtttat agcagcataa tatgtttcta cagttttata
       61 atgattatga tgaatggcta tatgaacagc tgacatattg tcatgatttt tagcggccaa
      121 caatgtgaat aactgacatt gactaaaaga atatttattt gcaaaagtac ccagcgttga
      181 tatataagat aacacgacgt ctttattacc cttgctcatg gcgatgtata aagcaggggt
      241 tccatatgct gttgcgcctt ttaataaatc catgatgtta attttgctga ggttatattt
      301 aacagcgatt ccataaactt tagagaggaa ccgtgtgaca cacaaagggt gattattttc
      361 cattgcggca tataatcctg gctcaccatc acgtgttttc attgctaaaa tatccatcac
      421 catttttttg cttaaaagct cttgtgttat tggagtggtt aatgcattca gaaagatatc
      481 gattatttca tcgaatccat acaacatgga gatatagagt ccaggtgttt cgttagcact
      541 tttagtttgc agtaatttaa ccagattatc ttcatgaatg atatgattat ctaccagtga
      601 ctgaatttct tgcataaata tttttacgtt gtcagtatgt ccgttcatca tagccagaaa
      661 taaagcaggt gtaccaacgc tattctgcgc agttattatt tcaatgattt ctttttcagg
      721 gagtatgggt aactgtttga ataatgcaat aagctgttga gtctggcctg tggccattat
      781 taacaacatt gccgatggat gtataggggc aaatggcatt cgtgatgcaa tacccgcgaa
      841 aatagcccct ggaactccct caggtaatag gggcagaaaa gcaattgcac agttgcgaac
      901 atacatatca gcccaggtct gagcatcggg acctgaactg atgaatttat cgagcgagag
      961 aaagccccgt gctgtcctgt gggtgcctct gacggcaacg ttggtatcgt tcgggtcgta
     1021 aacggacaca acataattta attgcccata cgctgactgc tttattcgta gccttactga
     1081 caaagtatga ttgtctatat ataaaatcgc ggacatatga ctgacattgt ttgccatcat
     1141 tcgacagaag aaaatatcta aagcgaagcc aaaatcctta ctcgcgataa cgcgactccc
     1201 tgcaggagga tggcgaagta atgatctgaa ctccgcgtcc cggactaagg gaacatgctg
     1261 ctgtatggat tcaacggagg agtaatgttt aatcagattg actttgcctt ttccatttct
     1321 cagtgaatca agtgcgtatt gcaatgacag atggcgacaa aaaatagcgt cagaaggaga
     1381 ttgcgaaaaa aatgcactac cattgagatt tactgttggt tgtatagttt tt
//
LOCUS       DACBVI010000306         1395 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.310, whole genome shotgun sequence.
ACCESSION   DACBVI010000306 DACBVI010000000
VERSION     DACBVI010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.310"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..491)
                     /locus_tag="IU325_22925"
     CDS             complement(<1..491)
                     /locus_tag="IU325_22925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811828.1"
                     /translation="MSPVSSIGNDISSDLARRKINDLPESPIVNNLEALAPGIEKLKQ
                     TSIEMVTLLNALQPGGKCIITGDFQKELAYLQNVILYNDSSLRMDFFGYNARIIQRSD
                     NTCELTINEPLKNQEISTGNINVNCPLKDIYNEIRRLNVIFSCGTGGIVDLSSLDLRN
                     VDL"
     gene            complement(513..1052)
                     /locus_tag="IU325_22930"
     CDS             complement(513..1052)
                     /locus_tag="IU325_22930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811829.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPPTYFNKTSIP
                     SDCWQILFFSPDHFNNDFY"
BASE COUNT          422 a          253 c          266 g          454 t
ORIGIN      
        1 tctaaatcga cattccgtaa gtccagagag gatagatcaa ctatacctcc agtcccacaa
       61 ctaaagatta cgttcaacct cctgatttca ttgtaaatgt ctttcaatgg gcaattaaca
      121 ttgatattgc ctgtggatat ttcctggttt tttaacggtt cattaatggt aagttcacag
      181 gtattgtctg atcgttgaat aattcgggcg ttataaccaa aaaaatccat acgaagagac
      241 gaatcattat aaagaattac attttgtaag tacgctaatt ctttttgaaa atcaccagtg
      301 ataatgcatt tcccacctgg ttgtagcgcg ttaagtaaag tgaccatttc aatagaggtc
      361 tgttttagtt tttctatacc tggtgctagt gcttcgaggt tattaactat ggggctttca
      421 ggaagatcat ttatttttcg acgggctaaa tcactactaa tatcattacc aattgatgaa
      481 acaggggaca ttattttatc ctttataaat aatcaataaa aatcattatt aaaatgatcg
      541 ggtgaaaaaa agagaatttg ccaacaatct gaaggaatgg aggttttatt aaagtacgta
      601 gggggggtta agatggcttc ttttcgggtg aattttgcac aatacttaga aatcgtctcc
      661 ttaatacact ccagctcttt tttattgacg gctgagcaag tacattgttc gccaaaaggt
      721 gcaaaagtgc ctacatctaa tgtttgtatg atataatcgt catgttctcc tgtagaaata
      781 tctatgatga atggactcat attttccggt tggattcgga tcatgacaga aggaacatcg
      841 ccttgataga aattcaatgc agtaattgca gataatactc tccgcagttc attacttata
      901 ctgtatattt tattaacgtc attggtggag acaatttctg caaactttct ttttaccgtc
      961 caataatcaa tatttctgtt gtgattgtta accactgcgt gcattggcgg gattgaaggg
     1021 tgaaaaatat gtgcaattgg gaagcctttc attgatgcct gccttgtcat catgacaaac
     1081 aataatagat gcttattttc ctcgcagggt tctttcgcac tatcaaaaaa cgctcattta
     1141 cccggcgtta cagtaaacga agcaacattc tgtggcctac gccgatcaaa agctctgtga
     1201 aaatttccct tgttgtcttt gtctgacctt atcgcggcga ccagcaatta ttcgttttgt
     1261 gatcctcaac acaaaatgct attcgtcgcc tatgagggga gaggaattta tggaaaacga
     1321 ttgcattcac aaaatgacat cctgtcagaa gagaaaaagt tgacgttaat caattgttcg
     1381 agatgatcaa tacct
//
LOCUS       DACBVI010000307         1385 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.311, whole genome shotgun sequence.
ACCESSION   DACBVI010000307 DACBVI010000000
VERSION     DACBVI010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.311"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..1340)
                     /locus_tag="IU325_22935"
     CDS             complement(<1..1340)
                     /locus_tag="IU325_22935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HAP5811830.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
                     AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY
                     YSLGLLNAWSLAGEHRHWMIPLRKGAQYEEIRKLGKGDHLVKLKTSPQARKKWPGLGN
                     EVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMGDLYSHRWEIELGYREIKQTMQRSR
                     LTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLM
                     TLQGASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQSV"
BASE COUNT          282 a          392 c          355 g          356 t
ORIGIN      
        1 actgactggc tctttttcgg ggctgtgggg tatttccagg gcctctcctt taccactctc
       61 gggaaggccc tttcccttct tgtcggtaat ttcacaagtt gtcccatact tgcaagatcg
      121 cgcatcagct ccggtatacg tcccggtgaa gcgccctgca atgtcatcag cattctcatc
      181 accattccgc atgattctga gaaactcagt tgattcggcc agtaaccttt cagatgttcc
      241 gccattttaa tcatctgata tctcaccaga ttataagcca gtaagacacc ccacagctct
      301 tgctccacaa gctccggctt tttacttctc agcgtcagcc tgctccgttg catcgtctgt
      361 tttatctccc tgtatcccag ttcgatttcc cagcgatgac tgtacagatc ccccatttct
      421 cctccgggga agcgcatggc gtccgtcatc gacgtcagca gatggcagac ttttcctttg
      481 cgcgtcacgg tcagcaggcg ggcagtcact tcatttccca gtcccggcca cttttttcgt
      541 gcctgcgggc tggttttcag cttcaccaga tgatcgcctt tacccagttt tctgatctct
      601 tcatattgcg ctccctttct gagaggtatc atccagtggc ggtgttctcc cgccaggctc
      661 caggcattta acagtcccag tgagtaataa cctttatcca ttaacgtcag agtgttatcg
      721 ccggtttgtt ctataagttg ctcagcaagc tcattttcgc tgttcttcat cgtgccgaag
      781 gctgcagccg tcagcagatg gctggtcagt tccatctggc agaccatttt gacctgcggg
      841 tagagcgccg ggttcccggc atgtgtctgg cgggggaagg ctgcatcgtt ctctggtgta
      901 tccggtgtgc gccagaacac accatcgatg gccagcaggg tcaggccgca ccagtgcgga
      961 tgcggcgtgg cgttatgcca gagctgcgct gttttcgtga acacgcggcg gacagcctca
     1021 cttcccaggc gctggcgggc ctgaataacg gcactggggg caacgaaggg gcgattgccc
     1081 ggcagcatga tgtccaggcg attcacaatc tggtgaagag gttctttacg ctcaagcgcc
     1141 atgccaacaa tacaccagac catcatttcg aggggaagac ggcgcttgcg tagcgttaca
     1201 gtacctgatt cggcaaggca acgagagatg agttcggggt cgaggtaatc ccccagagaa
     1261 gtcagtgggt tacgcagaga atcgtaacgg gataccagat caagagcctg tccaatgtgc
     1321 ataaaaaaat ccggaaacaa gtgagcgttt ccggattctt acacagccac tggatcggtc
     1381 aactg
//
LOCUS       DACBVI010000308         1336 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.313, whole genome shotgun sequence.
ACCESSION   DACBVI010000308 DACBVI010000000
VERSION     DACBVI010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.313"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            170..>1336
                     /locus_tag="IU325_22940"
     CDS             170..>1336
                     /locus_tag="IU325_22940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HAP5811831.1"
                     /translation="MAIFHLDFKIVKRSEGRSSVAKAAYHARCKITDERTGDTYDYSR
                     RTDLYGHFILAPVNAPEHIVKDSTALWNEVERVERQSNGQTARYFDVAIPAELNNDDK
                     KKLVLEYCQKNFVDKGMIADIAFHDLDSDNPHAHVMLTLKTIGPEGFGKKERSWNDRK
                     MSVLWRESWASIANSYLAAAGSSERIDHRSLQAQHEEALEKAAAALDNEEKALWLAKA
                     AEINRPAMKRIHSAKWRSKSAQEQRAAEQAVRDAAKQEAVEVYKTFSELDLEIIVDVR
                     SFTVAVLAEPEEIVLPEKHSGSSTHETQRPVLVAPVPHNRVRSVKSYRDKSKVSKVIA
                     GKKPSASISDSGTNTILKTSSSQNPNRASRSAPEHIKRQQTTTHQDNVFKRITTI"
BASE COUNT          412 a          270 c          288 g          366 t
ORIGIN      
        1 tgtggggtct accgacggtt gctgccgcag gctaacaccg tttccttcgg aaacaaggtc
       61 aacgtcaaca ccaaagtttt tcttacgaaa aactaaaacc agcatagtcg cattggagat
      121 ttatattttg gtttgatatt taaattcata ttgaggttgt ttttattgta tggctatttt
      181 tcatcttgat tttaaaattg tgaaacgttc tgaaggtagg tcttccgttg cgaaagctgc
      241 ttaccatgct cgttgcaaaa tcacagatga aagaacgggt gatacctatg attatagccg
      301 ccgtaccgat ttatacgggc atttcatatt agcgccagtt aatgctccag aacatatcgt
      361 aaaagattct acagcattgt ggaatgaagt tgaaagggtt gagcgtcaga gcaacggaca
      421 aacagctcgt tatttcgatg tcgccattcc tgccgagtta aataatgacg acaagaaaaa
      481 acttgttctt gagtattgcc agaaaaactt cgttgataaa ggcatgattg ccgatatcgc
      541 tttccacgat cttgatagtg ataatcctca tgctcacgtt atgttgacat taaaaaccat
      601 aggccctgaa ggttttggta aaaaagaaag aagctggaac gacagaaaaa tgtctgtgtt
      661 gtggcgtgaa tcatgggcat ctatagctaa cagctatctt gctgctgctg gttcatcaga
      721 acgtattgat catcgttctc ttcaggccca gcacgaggaa gcgttagaaa aagcagcagc
      781 agctttagat aatgaagaaa aagcgttatg gcttgctaag gccgctgaaa taaatcgtcc
      841 tgcaatgaaa cgtatccaca gcgctaaatg gcgtagtaag tctgctcagg agcaaagagc
      901 cgcagaacag gctgttcgtg atgcagctaa gcaggaagcg gttgaagtct acaaaacatt
      961 cagtgaactt gatcttgaaa tcattgttga tgtcagaagc tttactgtgg ctgttcttgc
     1021 tgaaccagaa gagatcgtat taccagaaaa gcactccggc tcttctactc atgaaacaca
     1081 aaggcctgta ttggtagcac cagttcctca taatcgtgtg cgtagtgtca aatcttaccg
     1141 tgataaatcc aaagtcagta aggtcattgc tggtaagaaa ccatcggcaa gtatttctga
     1201 ttctggaaca aatactattc tgaaaacatc ctcctctcaa aacccaaaca gggcatccag
     1261 aagcgcccct gaacatatta agaggcaaca gactactaca catcaagata atgtttttaa
     1321 acgtatcact accatt
//
LOCUS       DACBVI010000309         1323 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.314, whole genome shotgun sequence.
ACCESSION   DACBVI010000309 DACBVI010000000
VERSION     DACBVI010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.314"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..1304)
                     /locus_tag="IU325_22945"
     CDS             complement(<1..1304)
                     /locus_tag="IU325_22945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HAP5811832.1"
                     /translation="MNTSHVRVVTHMCGFLVWLYSLSMLPPMVVALFYKEKSLFVFFI
                     TFVIFFCIGGGAWYTTKKSGIQLRTRDGFIIIVMFWILFSVISAFPLWIDSELNLTFI
                     DALFEGVSGITTTGATVIDDVSSLPRAYLYYRSQLNFIGGLGVIVLAVAVLPLLGIGG
                     AKLYQSEMPGPFKDDKLTPRLADTSRTLWITYSLLGIACIVCYRLAGMPLFDAICHGI
                     STVSLGGFSTHSESIGYFNNYLVELVAGSFSLLSAFNFTLWYIVISRKTIKPLIRDIE
                     LRFFLLIALGVIIVTSFQVWHIGMYDLHGSFIHSFFLASSMLTDNGLATQDYANWPTH
                     TIVFLLLSSFFGGCIGSTCGGIKSLRFLILFKQSKHEINQLSHPRALLSVNVGGKIVT
                     DRVMRSVWSFFFLYTLFTVFFILVLNGMGYDFLTSFATVAAC"
BASE COUNT          503 a          289 c          213 g          318 t
ORIGIN      
        1 atacatgcag ccactgttgc aaatgatgta agaaaatcat atcccatacc atttaacacc
       61 agtataaaaa acaccgtgaa gagagtataa agaaaaaaga aactccatac agacctcatt
      121 acacgatctg taactatctt ccctcctaca tttacactca acaacgctct gggatgagaa
      181 agctgattta tctcgtgttt gctttgtttg aaaagtataa gaaatcgaag tgacttaatt
      241 ccaccacaag ttgaacctat acatccccca aagaaacttg acaacagcaa aaacactatc
      301 gtgtgcgtgg gccagtttgc ataatcctgc gtagctaaac cattatcagt gagcatggag
      361 ctggcaagaa aaaacgaatg aataaaactt ccatgcaagt catacatacc tatatgccag
      421 acctggaaag aggtaacaat gatcacccct aaggctatta acagaaagaa acgaagttca
      481 atatctctga ttaaaggttt tatcgttttc ctgctaataa caatatacca aagagtgaag
      541 ttgaaagccg atagcaggga aaaagaacca gccaccagct caaccaaata gttattaaaa
      601 tatccgatac tctcgctatg agttgagaaa ccaccaagcg aaactgtgga aatcccgtga
      661 caaatagcat caaacaaagg cattcctgca agtctataac agacaataca agcaatacct
      721 aataaagaat aagttatcca cagtgtccgt gacgtatcgg ccaggcgggg agtgagtttg
      781 tcatccttaa atggccccgg catttctgac tgataaagct ttgcaccacc aatacccaat
      841 aatggcaata cagcaaccgc cagaacaata actcctaaac cacctataaa atttaactgt
      901 gaccgatagt acaaatatgc ccgaggtaat gaactaacat catcaattac agttgctcct
      961 gttgttgtta ttccagaaac cccttcaaac agagcatcaa tgaacgttaa attaagttct
     1021 gagtcaatcc ataaagggaa tgcactaata acagaaaaca aaatccaaaa cattacaatt
     1081 ataataaacc catcacgggt acgtaattga atgccagatt tcttagttgt ataccacgct
     1141 ccgccaccaa tgcaaaaaaa tataacgaaa gttataaaga aaacgaatag gcttttttct
     1201 ttataaaaca atgctacaac cattggtggc aacattgaaa gactatagag ccaaaccagg
     1261 aacccacaca tatgagtaac aactcttaca tgagatgtat tcatatctaa atattctttc
     1321 aat
//
LOCUS       DACBVI010000310         1318 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.315, whole genome shotgun sequence.
ACCESSION   DACBVI010000310 DACBVI010000000
VERSION     DACBVI010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.315"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..835
                     /gene="yeeO"
                     /locus_tag="IU325_22950"
     CDS             <1..835
                     /gene="yeeO"
                     /locus_tag="IU325_22950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HAP5811833.1"
                     /translation="PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFK
                     PLNFSIIWEVMGIGIPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINL
                     PGSALGSASTIITGRRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFY
                     TQDPQVKHVVVILIWLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVV
                     GYVLGIMLGWGVVGVWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPAVS
                     E"
     gene            936..1011
                     /locus_tag="IU325_22955"
     tRNA            936..1011
                     /locus_tag="IU325_22955"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:969..971,aa:Asn,seq:gtt)
BASE COUNT          297 a          265 c          350 g          406 t
ORIGIN      
        1 gccgggactg ggatttgtcg gggctgggtt gggtttaacc atttctcgtt atattggcgc
       61 agttgcaatt ttgtgggtgt tggcgattgg ttttaatcct gcgctaagga tttcgttaaa
      121 gagctatttt aaaccgctga attttagcat tatctgggaa gtcatgggga ttggtattcc
      181 cgcgagtgtc gaatcagtgt tatttaccag tggtcggtta ttaacccaaa tgttcgttgc
      241 cgggatgggg accagtgtta ttgccggaaa ttttatcgcg ttttcaattg cggctcttat
      301 caacttaccc ggaagtgcgc tcggctctgc ttctacgatc attacaggcc gaaggttggg
      361 tgtaggacag atagcgcaag cagagattca gttgcggcat gtgttctggc tttccactct
      421 tggattaacg gccatcgcct ggctaacggc tccctttgcc ggggttatgg catcgtttta
      481 cacccaggat ccacaggtta aacatgtcgt tgtgattctg atttggctaa atgctttatt
      541 tatgcctatt tggtccgcct catgggtgct gcccgctgga tttaaggggg ctcgtgacgc
      601 ccgttacgct atgtgggttt ctatgttgag catgtggggt tgccgggttg tcgtcggtta
      661 tgtgctgggc atcatgcttg gctggggtgt ggttggtgtc tggatgggaa tgtttgccga
      721 ctgggctgtg cgggccgtgc tgttttactg gcgaatggtt actggacgtt ggctatggaa
      781 ataccctcga cccgaaccgc aaaagtgtga aaaaaagcca gctgtgtcgg aataaacgac
      841 aaaatgcaga ttatttcagc aaacgatttc aaatttaaaa aacaggcttt gacattgtgg
      901 ttgggcatcg ctaatattcg cctcgttctc acgattcctc tgtagttcag tcggtagaac
      961 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aaattttagt
     1021 tttgagacat ctctatgaat ctcaaaacag ttaaaaaata aaaattattt ttacccggtt
     1081 accttgtaaa aaagtgaccg ggttttttga tttctgtgtt tcgtgcgttt aatcgagtct
     1141 cacgcttcag tgagcaaatt gcccccaaca gacatcacga ttcgcaaggt caaaatctct
     1201 gttccatgaa gtaccgtaca aagataaaga gaacaatgcc ttatgcggga tgtcgctgtc
     1261 gcgtctttat cgggaaacta tacgtaattg ccactttgtc ttcggtaatc agaaaaag
//
LOCUS       DACBVI010000311         1315 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.316, whole genome shotgun sequence.
ACCESSION   DACBVI010000311 DACBVI010000000
VERSION     DACBVI010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.316"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(72..398)
                     /locus_tag="IU325_22960"
     CDS             complement(72..398)
                     /locus_tag="IU325_22960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811834.1"
                     /translation="MKYIIFLFRAIWLALSLLILFFSMHRLSLLDSTRDVSELISLMS
                     YGMMVICFPTGIVFFIALIFIGTVSDIIGVRIDSKYIMAIIIWLYFLSGGYIQWFVLS
                     KRIINK"
     gene            complement(503..1315)
                     /locus_tag="IU325_22965"
                     /pseudo
     CDS             complement(503..1315)
                     /locus_tag="IU325_22965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          384 a          297 c          222 g          412 t
ORIGIN      
        1 cctgccgggt ccgtggcata cgggatatca atgcccaggt tacatataat aataagagca
       61 attaacttaa tttatttgtt tataatgcgc ttacttaata caaaccattg aatataccct
      121 cctgacagaa aataaagcca tattattatc gccataatat acttactatc aatccttacg
      181 ccaataatgt ctgaaacagt ccctataaat attagagcaa taaagaatac tatgcctgtt
      241 ggaaaacaga ttaccatcat tccataagac atgagactaa ttaactcgct cacatcacga
      301 gtggagtcta atagtgaaag tctatgcatg gaaaagaata gtattaaaag cgataaagcc
      361 agccatatag ctctgaataa aaaaataata tatttcatta catcactccg gtgaatacaa
      421 atctaataaa gcgtccaatg ttatttccgg agcacttcat cttacatgtt tctaaagaat
      481 cattagacca gtcagggcaa gattacgaaa gcccgctccc cgcaaggact gacgcgagat
      541 agtttctgtc catggctgct tttcgcatct tacgtcttaa ccctaccttg aataccttat
      601 cattcgtcaa aatattaata gcaatgtgcc gtatccctga aaataattct gctgcgtttc
      661 ctcttcttat tttgcagtcg tcttcattca ttaccacgtc cagacgccag tgcagcttat
      721 tctccacgtg ccagtggttt cggattgctg tggcgaactt ctcagcggtt aaatcagcag
      781 agctgatata atatctgacc gtcatttctg gctctttctt ttgttctgct attattgacc
      841 gaaaggagac tgccacgtat aatttttcag tcctttccat tcaaatgtaa aatcaataag
      901 ttcatcaggg acatcgcaaa caatatgaag acggatttct tctctgccgt gactcttttc
      961 actaattgcg taactgtcat gctctggatt aattaattct ttcagcggaa atgtttcctc
     1021 aaaggcttta ttcagccgcc cctggtttcc ttttacagcg aataagtaat caccaccctg
     1081 tttttgtatc ttctctgcaa tatctttctg gcaacccatc gcatcagttg tgatgatttt
     1141 ctctttaata tacaaaatgt taagaagttc agggatagct gtgatctcat tagatttctc
     1201 atccgtcctg atctgcccca ggaccagaat gtgcattgtt gagaacgcac taatgacatg
     1261 aatcgctccc ctgcggcgac tcttgtcata agaatgccgg agtgtttttc catca
//
LOCUS       DACBVI010000312         1302 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.317, whole genome shotgun sequence.
ACCESSION   DACBVI010000312 DACBVI010000000
VERSION     DACBVI010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.317"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..318)
                     /locus_tag="IU325_22970"
     CDS             complement(<1..318)
                     /locus_tag="IU325_22970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HAP5811835.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHYRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLADAGVFR
                     WVTD"
     gene            complement(539..1270)
                     /locus_tag="IU325_22975"
     CDS             complement(539..1270)
                     /locus_tag="IU325_22975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP5811836.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIEAPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          332 a          303 c          294 g          373 t
ORIGIN      
        1 atcagtaacc caccggaaaa cgcccgcgtc tgccagtgtt gaacagtatt cccgaaatgt
       61 tgattttccg caaatatgaa gcaatgcggc ctcttttatt ttagcagggt tcttggtcgt
      121 actaactttt aacaggttcc tggttcctct taataacaaa accgtgtcat cgtgagtaat
      181 aattctgatg ttatccgtag ccagataata aatgtaatgt gcaatacggt aatgttttaa
      241 ttctgaataa aaccaggaga agttttgctc ttttctcact tgctcaaaca tcttttgaaa
      301 aacaacaacc tgatccatca ggataataac ctcttgttag ttgtgagact gcgtagtgtg
      361 cacgatcggt tttactactt caatctggtc tgtcctttgg ctgtgatatg tacaaagtgt
      421 gaaaggggga ttacctgaat tctcccggtg agcattttgc aacggaccag ctccggtaca
      481 aacgctgttg tgggttcaga ttataagatt ctgtctaagg ggcgggataa aggtgaaatt
      541 agggggcatg aaagatgact ttataacctt gctcacccca gtgttgtaaa agttcgtttt
      601 gccttctcgt tggtgccatg cctgtccaga caatcaatgt ttgcgttggg aacagttccg
      661 ggcgcggcgc ttcaatggga tcagcaagaa cagaaatgtg ccatcctgac aatgataaac
      721 gccatgcttc cagccacaat cgtgctctct cttcaacatt ccacgccatc agaatggctt
      781 ctttaccggg cttacggcgc atttcgaaaa gcgaggctgc tgcgtattca attaatgcgc
      841 cgtcaaacat actgctcata atgcgggagg tgttgtgatc aagcacgaga cgctggcgaa
      901 caggaaggta aacatgatta atcaattgat ctactgggta ctctcgaccc agtgaaataa
      961 ttctcgcgcg tagtttggca ggattagcca tacgaagaat tgacatcatc tcttcttgca
     1021 ggcggctcca gtcatcttcc gtatcctggc tggtggtttc cagtaatgct ttaactttgc
     1081 ctacagggac gccattactt atccaacgct tgatctcttc gatgcgttgt atgtcttctt
     1141 catcaaagag tcggtgtccg ccttcactgc gctgtggttt taacaaaccg tagcggcgtt
     1201 gccaggcccg gagagtgaca ggattaatcc cgcaacgttc agcaacatca ccaatgctgt
     1261 aataagccac aattcctcct tgcggtcaca aatctccgtc gc
//
LOCUS       DACBVI010000313         1288 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.318, whole genome shotgun sequence.
ACCESSION   DACBVI010000313 DACBVI010000000
VERSION     DACBVI010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.318"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..241
                     /locus_tag="IU325_22980"
     CDS             <1..241
                     /locus_tag="IU325_22980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP5811837.1"
                     /translation="YKYPKYSFFVRDVINKSINEIIEKTEINQLSFSVVGKKGRMAHM
                     LRFEFSINEKSSSFSEDDMAFLEEFDKVVPPKKNK"
     gene            250..693
                     /locus_tag="IU325_22985"
     CDS             250..693
                     /locus_tag="IU325_22985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811838.1"
                     /translation="MAKIYEFPQGAERSKLKKEIIRERKKRLRETNGNPVIRHAKWFW
                     FYLRLATAGALHLVSVISLAVLGAFSKAIFWIGGMLCVVTWFHLERQFWTPQNFTIPV
                     IVTLWGLSLFATPLMELLNKKMPWYRLLVPDAKHTSTEATNDDQP"
     gene            881..1234
                     /locus_tag="IU325_22990"
     CDS             881..1234
                     /locus_tag="IU325_22990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 3"
                     /protein_id="HAP5811839.1"
                     /translation="MKYQVKEFINEKYSKAVNILKDNLKEHYHIFYGLRLSEILFPAN
                     EYGSEMFFQEFEAINSVILPLVIFDLIDRKPIMVIGFGEVCGVDSLVDSGIEVVSLDG
                     LSDLLLVEKLTPLFN"
BASE COUNT          365 a          230 c          287 g          406 t
ORIGIN      
        1 atataagtat cctaaatact cattttttgt tcgtgatgtt atcaataaaa gcattaatga
       61 gataattgag aaaacggaaa taaatcagtt atctttctct gttgttggga aaaaagggag
      121 aatggctcac atgcttagat ttgagtttag tataaatgaa aaatcctcat ccttttcgga
      181 agatgatatg gcatttttag aggagtttga caaagtcgtt ccaccaaaaa agaataagtg
      241 aggcaaaaca tggctaaaat atatgagttt ccgcagggag ccgaacgaag taagctgaaa
      301 aaagaaatca tccgggaacg taaaaaacga ttgcgagaaa cgaacggtaa cccggttatc
      361 cggcacgcta aatggttctg gttttatcta cgtcttgcta cggctggcgc actgcattta
      421 gtctctgtta tttctctggc agtcctgggg gctttcagta aagctatttt ctggattggt
      481 ggaatgctct gtgttgttac atggttccac cttgagcgcc agttctggac gccgcaaaat
      541 ttcacgattc cagtaatcgt aacgctgtgg ggattgagtt tgtttgccac tcccctgatg
      601 gagctgctta acaaaaaaat gccgtggtat cgtcttctgg ttcctgatgc aaagcacact
      661 agcactgagg caaccaatga tgatcagccg tgaagcaaca atgttatcca gtcggcttgc
      721 agtgcttctt tcagcctttt atcatcggtt aatcatagcg cacgaggcga gttcttttca
      781 ctgcgatgca ggatagggca aacgccgcaa aatgacgtct ctgacgccat tccgcaacgt
      841 ttgcccggtg acctacagtc ggttcttgtt ggagagagtt atgaaatatc aggttaaaga
      901 gtttattaac gaaaaatatt ctaaggctgt taatatttta aaggataacc ttaaagaaca
      961 ctaccatatt ttttatggtt tgagattaag tgagattctt tttcctgcca acgaatacgg
     1021 ctctgaaatg ttttttcagg agtttgaagc gattaacagt gtaatattgc ctttggtgat
     1081 ttttgatttg atagaccgaa agccgattat ggttatcggc ttcggtgaag tatgtggagt
     1141 tgattcactt gttgattctg gtatagaagt tgtttcttta gacggtttaa gcgatctgct
     1201 actggttgaa aaactgacgc cattatttaa ctaaacctct ggataactat cttttcagtt
     1261 ttccagtaag attttttcta tatctgaa
//
LOCUS       DACBVI010000314         1284 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.319, whole genome shotgun sequence.
ACCESSION   DACBVI010000314 DACBVI010000000
VERSION     DACBVI010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.319"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..581)
                     /locus_tag="IU325_22995"
     CDS             complement(<1..581)
                     /locus_tag="IU325_22995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HAP5811840.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEDW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIRTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFA"
     gene            complement(685..996)
                     /locus_tag="IU325_23000"
     CDS             complement(685..996)
                     /locus_tag="IU325_23000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HAP5811841.1"
                     /translation="MEIVIYITISILILAIALIKQRMLANIETSNPYHAQYILRRRDD
                     YLVLFDRDALSCRFFYDAFPGMRLRHQVTGDTDDDRLAHSPADRMYTGKDHQKYTFCT
                     K"
BASE COUNT          376 a          269 c          249 g          390 t
ORIGIN      
        1 gcgaataaat aatcacctcc ctgtttttgt atcttctctg caatatcttt ctggcaaccc
       61 atcgcatcag ttgtgatgat ttttccttta atatccagca tgttaagaag ttctgggata
      121 gctgtaatct cattggattt ctcatccgtc ctgatctgtc cgatgaccag actgtgcatt
      181 gttgagaacg cactaatgac atgaatcgct cccctgcggc gactcttgtc ataagagtgc
      241 cggagcgttt ttccatcaat tgcaatgacg tctttatcat ctgaagaatg gcagtcacgc
      301 atccagttaa taaagcactc gtgaaatttt gcaggactga tacaggatac aactctggca
      361 atggtatcgt gaacaggaat accattttca aaatcaccat attgcttcaa aaaatcgaga
      421 tgtgtttccc caaaatcctc tatatcttcc caatcttccg caccagaaat aacggcacaa
      481 atagtcaaca atagaatatc cgataattta tgttccactt tccaggcttg tctgtaatcg
      541 ggaataatag aaatatgttc catcaatttt ttaagttcca ttttgttctc cttaattatg
      601 taaggagtat ttgatcatgt ataagcaata aaaaacagct tcaggtaatg aggaatatct
      661 caatttttaa acataaaatg tgaattattt agtacaaaaa gtgtacttct gatgatcttt
      721 ccctgtatac atccggtctg cggggctgtg tgccaggcgg tcatcgtcgg tatcgccggt
      781 cacctggtgg cgcagacgca ttcccggaaa ggcgtcataa aagaagcggc agctcagggc
      841 atcacggtcg aacagcacca ggtaatcatc gcggcggcgc aggatgtact gggcgtgata
      901 cgggttactg gtttcgatat tggctaacat ccgctgtttt attaatgcga tagctaaaat
      961 caggatggaa atagtgatgt atatcactat ttccatgatc ttgccttgcg ttcatccagg
     1021 caggaatatc cctgttagca gcctctgtgc gatgtacgcc agcggtctgt aaagaaaccg
     1081 attaccttat aacaaaacct ctaacgtagt ttataataca tcaggaatat taataaaacc
     1141 ttctgggtca gatatctcaa taaatatcat atcaggctca aaagtttcag ggacttttaa
     1201 tttcgctgaa attaaccaac cttgcttaga accaatacta atagatttat agctgtaatt
     1261 ggcatgtaga ctatttttaa tata
//
LOCUS       DACBVI010000315         1272 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.320, whole genome shotgun sequence.
ACCESSION   DACBVI010000315 DACBVI010000000
VERSION     DACBVI010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.320"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..>1272
                     /locus_tag="IU325_23005"
     CDS             <1..>1272
                     /locus_tag="IU325_23005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811842.1"
                     /translation="RAGRPEIRYRYDSDGRVTEQINPAGLSYTYQYEKDRITITDSLD
                     RREVLHTQGEGGLKRVVKKEHADGSVAQSQFDAVGRLKAQTDAAGRTTEYSPDVVTGL
                     ITRITTPDGRASAFYYNHHSQLTSATGTDGLEIRREYDESGRLIQETAPDGDITRYRY
                     DNPHSDLPCATEDATGSRKTMTWSRYGQLLTVTDCSGYVTRYDHDRFGQVTAVHREEG
                     LSQYRAYDSRGQLIAVKDTQGHETRYEYNAAGDLTAVIAPDGSRNGTQYDAWGKAIRT
                     TQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGK
                     LIRSEDEGLVTHWHYDEADRLTHRTVKGEPAEQWQYDERGWLTGISHLSEGHRVTVHY
                     GYDEKGRLTGERQTVHHPQTEALLWQHETRHA"
BASE COUNT          316 a          368 c          392 g          196 t
ORIGIN      
        1 ccgcgcgggc cggccggaaa tccgttaccg ttacgacagc gacgggcggg tgacagagca
       61 gataaacccg gcaggcttaa gctacacgta tcagtatgag aaagaccgca tcaccatcac
      121 cgacagcctg gaccgccgtg aagtgctgca cacgcagggc gaaggcgggc tgaagcgggt
      181 ggtgaaaaag gaacacgcgg acggcagcgt cgcgcagagt cagtttgatg cggtgggtag
      241 gcttaaggca cagacggatg ccgcaggccg gacaacagaa tacagcccgg atgtggtgac
      301 gggcctcatc acgcgcatca ccacgccgga tggcagggca tcggcgtttt actataacca
      361 ccacagccag ttaacgtcag ccaccgggac tgacgggctg gaaatacgcc gggaatatga
      421 tgaatccggc cgtctgattc aggaaactgc ccctgacggc gatatcaccc gctaccgtta
      481 tgataatcca cacagtgact taccctgcgc aacggaggat gccaccggca gccggaaaac
      541 catgacatgg agccgttacg gtcagttgct gaccgtcact gactgttccg gttatgtaac
      601 ccgttatgac catgaccgtt ttggtcaggt gacggcggtg caccgcgagg aagggctgag
      661 tcagtaccgc gcatacgaca gccgtggaca gttaattgcg gtgaaagaca cgcagggcca
      721 tgaaacgcgg tatgaataca acgccgccgg tgacctgacc gccgtcattg ccccggacgg
      781 cagcagaaac gggacacagt acgatgcgtg gggaaaggcc atccgtacca cacagggcgg
      841 tctgacgcgc agtatggaat acgatgctgc cggacgggtc atccgcctga ccagtgaaaa
      901 cggcagccac accaccttcc gttacgatgt actcgaccgg ctgatacagg aaaccggctt
      961 tgacggccgc acacagcgtt atcaccacga cctgaccggt aaacttatcc gcagcgagga
     1021 tgaggggctg gtcacccact ggcactatga cgaagcagac cgcctcactc accgcaccgt
     1081 gaagggtgaa cctgcagagc agtggcagta tgacgaacgc ggctggctga caggcatcag
     1141 ccatctcagc gaaggccacc gggtgacggt gcattacggg tatgatgaga aaggccgcct
     1201 gaccggtgag cgccagacgg tgcatcaccc gcagacggaa gcactgctct ggcagcatga
     1261 aaccagacat gc
//
LOCUS       DACBVI010000316         1257 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.321, whole genome shotgun sequence.
ACCESSION   DACBVI010000316 DACBVI010000000
VERSION     DACBVI010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.321"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     repeat_region   117..938
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=117..145
                     /rpt_unit_seq="gagttccccgcgccagcggggataaaccg"
     repeat_region   951..1163
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=952..980
                     /rpt_unit_seq="gagttccccgcgccagcggggataaaccg"
BASE COUNT          300 a          315 c          345 g          297 t
ORIGIN      
        1 aatcaatgaa ttaagtgctc tttaacataa tggatgtgtt gtttgtgtgt ttctgttaag
       61 ttggtagatt gtgactgact taaaaaatca ataattaata ataggttatg tttagtgagt
      121 tccccgcgcc agcggggata aaccgtttgc tgagtgtgca tacacacttg aacatcagag
      181 ttccccgcgc cagcggggat aaaccgctat cagtaacaac ctggtaaata tcggttttga
      241 gttccccgcg ccagcgggga taaaccgttt tcggggccat aaatggcgct catttatgtg
      301 agttccccgc gccagcgggg ataaaccgga tactttatta gcgaaagtaa tcctagctct
      361 gagttccccg cgccagcggg gataaaccgt tggggctggg gttcggtaat cagattattg
      421 agagttcccc gcgccagcgg ggataaaccg cggcaccagg gctgaaaggc ggggccgcta
      481 aagagttccc cgcgccagcg gggataaacc gggattctgc actctttgat gtaatggtac
      541 tgcgagttcc ccgcgccagc ggggataaac cgccattctt gttgtcgttc atcaagcgca
      601 gggggagttt cccgcgccag cggggataaa ccgtttgttt tgatgattta tcagtatatg
      661 aattggagtt ccccgcgcca gcggggataa accgattttt gcgcggcctg catgaaacca
      721 gtggcaaagt tccccgcgcc agcggggata aaccggtacc gttgagcaat ggtgggtaaa
      781 tggtgctgag ttccccgcgc cagcggggat aaaccgattt agatacggca cacaggaatg
      841 cgctggatga gttccccgcg ccagcgggga taaaccgcat gggagtccag attacaaatt
      901 ccagaatggg agttccccgc gccagtctga ggttttccgg tcatacatcg tgagttcccc
      961 gcgccagcgg ggataaaccg accgttgcta cacatatgat caggcatgtg ccgagttccc
     1021 cgcgccagcg gggataaacc ggacgatgag acgccctggt gtgccgcgtt cgtgagttcc
     1081 ccgcgccagc ggggataaac cgcgtttagc tccgcaatgt tggatatcac tgatgagttc
     1141 cccgcaccag cgggaataaa ccaccatata acccgttatc tctttctcaa gtttttatat
     1201 tagcagtact cgtattaagc aacatatcca cgtaacacct catgttcaaa atagctc
//
LOCUS       DACBVI010000317         1227 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.322, whole genome shotgun sequence.
ACCESSION   DACBVI010000317 DACBVI010000000
VERSION     DACBVI010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.322"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            69..>1227
                     /gene="bluF"
                     /locus_tag="IU325_23010"
     CDS             69..>1227
                     /gene="bluF"
                     /locus_tag="IU325_23010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HAP5811843.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQAAYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKEGEIYTAD
                     LKSKALAFAMAHALDLGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLF"
BASE COUNT          342 a          269 c          274 g          342 t
ORIGIN      
        1 aagaaagaca ccggaagata tgtctgttac cgtcttactc tcgcctctcc cattaccctg
       61 gacagttaat gcttaccacc cttatttatc gtagccatat acgtgacgac gaacctgtca
      121 aaaaaatcga agaaatggtt tcgatagcaa atcgcaggaa catgcagtct gacgtaacag
      181 ggatcttact gtttaatgga tctcattttt tccagcttct ggaaggtccg gaagaacagg
      241 ttaaaatgat atatcgggct atatgccagg atccacggca ctataatatt gttgagctgc
      301 tgtgcgatta cgcgcctgct cgccgttttg gcaaagcggg aatggaatta tttgatttgc
      361 gcctgcacga gcgagatgac gttttacagg ccgtattcga caaaggcaca tcaaaatttc
      421 agctaactta tgatgacaga gcgctacaat tttttcgtac ttttgtcctt gcaaccgaac
      481 aagcagccta tttcgagatc cctgccgaag actcctggct ttttatcgct gacggatctg
      541 ataaagaact tgattcctgt accctttcac caactataaa cgaccacttt gcctttcatc
      601 ctattgtcga tcccttatcg cggcggataa tcgcttttga agccattgtg caaaaaaatg
      661 aagacagccc gtcagctata gcggttgggc agcgtaaaga aggggaaatc tatacagcgg
      721 atctcaaaag taaggcgctt gcattcgcga tggcacacgc acttgacctc ggtgataaaa
      781 tgatttcaat caatctatta cctatgaccc tggttaacga acctgacgca gtctcttttt
      841 tacttgatga aataaaggcc aatgctctgg tgcctgaaca aatcatcgtt gaatttactg
      901 aaagtgaagt catatctcgg tttgatgagt ttgcagaagc gattaaatcg cttaaggctg
      961 ccggtatcag tgtagcaatt gatcattttg gcgcaggttt tgccggtttg ttactcctgt
     1021 cacgcttcca gcctgacaga attaaaatca gtcaggaatt gattaccaat gttcataaaa
     1081 gcgggccacg gcaggcaatt attcaagcga tcataaaatg ctgtacatca cttgaaattc
     1141 aagtcagtgc tatgggcgtg gcaacaccag aagagtggat gtggcttgaa tccgcaggaa
     1201 ttgagatgtt tcagggagat ctgtttg
//
LOCUS       DACBVI010000318         1193 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.323, whole genome shotgun sequence.
ACCESSION   DACBVI010000318 DACBVI010000000
VERSION     DACBVI010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.323"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..813
                     /gene="tnpB"
                     /locus_tag="IU325_23015"
     CDS             <1..813
                     /gene="tnpB"
                     /locus_tag="IU325_23015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP5811844.1"
                     /translation="GWMRYRNSRQVTGVVKNVTVSQSCGKWYISIQTESEVSTPVHPS
                     ASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQKRK
                     IQRLHSRIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAK
                     SGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVSPAYTSQRCTCCGHTAKENRLSQSK
                     FRCQVCGYTANADVNGARNILAAGHAVLACGGMVQSGRPLKQEPTEMIQATA"
     gene            complement(858..986)
                     /locus_tag="IU325_23020"
                     /pseudo
     CDS             complement(858..986)
                     /locus_tag="IU325_23020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
BASE COUNT          306 a          292 c          326 g          269 t
ORIGIN      
        1 ggctggatgc gctaccggaa tagccgtcag gtcacgggtg ttgtgaaaaa tgtcacggtc
       61 agccagtcct gcggtaagtg gtacatcagt attcagacag aaagtgaagt atcaactccg
      121 gttcaccctt cagcatcaat ggtcgggctg gatgctggcg tggctaaact cgccacgctg
      181 tcagatggca cagtctttga gcctgtaaac agttttcaga aaaaccagaa gacgctggcg
      241 agacttcagc gacagttaag ccgcaaggtc aaattcagca acaactggca gaagcagaaa
      301 cgcaaaatac agcgactgca ttcccgtatc gcaaatatcc gcagggacta ccttcacaaa
      361 gtcacaacga ccgtcagcaa aaaccacgca atgatagtca ttgaggattt gaaggtcagc
      421 aacatgtcaa agtcagcagc gggtacggtc agccagccgg ggcgcaatgt ccgggcaaaa
      481 tcaggtttaa accgttcgat actggatcag ggctggtatg aaatgcgccg ccagcttgag
      541 tacaagcagc tctggcgtgg cggtcaggtg cttgctgttt cgccagcgta cacaagccag
      601 cgttgcacgt gctgtggtca tacagcgaaa gaaaatcgcc tgtcacaaag taaattcaga
      661 tgccaggtat gtggatatac agcgaacgcc gatgtaaatg gcgctcgtaa cattttagcg
      721 gcggggcacg ccgttcttgc ctgtggaggg atggtgcagt caggccgccc gttgaagcag
      781 gaacccaccg aaatgattca ggcgacagcc tgaacgtagc agggatccac gtccttcagg
      841 gcgtggagga tgtcaatgag ttgcgtcatc tcctggttca gcgtgctgac gctgtgtttc
      901 atctgtcgca cttcgctttg ctgcacacag gcgctaaggc tgaacaggct tcccagcagg
      961 agcagtcgaa aaacgttagt attcataggt cgtccctttc tcgtcgcaat tagtctaatg
     1021 gtagagggta aaagcagtga aagcatcgat cctttatctc aaaagcgttg cgcctttgtt
     1081 gtatcgtcag ttcagggtaa aatagatttc tgttaaccac ctggtcagga cgccgtatgc
     1141 attgcccatt ctgtttcgcc gtggacacta aggtaattga ctctcgtctc gtg
//
LOCUS       DACBVI010000319         1190 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.324, whole genome shotgun sequence.
ACCESSION   DACBVI010000319 DACBVI010000000
VERSION     DACBVI010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.324"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..88
                     /locus_tag="IU325_23025"
     CDS             <1..88
                     /locus_tag="IU325_23025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enterotoxin"
                     /protein_id="HAP5811845.1"
                     /translation="NQDTNNSSFSYGDNRFEKSQGSKWTFQE"
     gene            102..341
                     /locus_tag="IU325_23030"
     CDS             102..341
                     /locus_tag="IU325_23030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
                     /protein_id="HAP5811846.1"
                     /translation="MADWNGYIMDISKQFDQGVDDLNQQVEKALEDLATNPSDPKFLA
                     EYQSALAEYTLYRNAQSNVVKAYKDLDSAIIQNFR"
     gene            361..693
                     /locus_tag="IU325_23035"
     CDS             361..693
                     /locus_tag="IU325_23035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811847.1"
                     /translation="MSVSNMPPIDRAEQSAAREIQQAKVIDLNDRVLNLDNPDDKMIS
                     AFANYAVQTENWQQNALQALRSDKKGLTPEKLLVLQDHVLNYNVEVSLVGTLARKIVA
                     AVETLTRS"
     gene            690..>1190
                     /locus_tag="IU325_23040"
     CDS             690..>1190
                     /locus_tag="IU325_23040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HAP5811848.1"
                     /translation="MKYISLFLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIYVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIVSSRVHVSYDVDTGDSGKTALPIHISVLAVYEKDINPEI
                     KINDIKR"
BASE COUNT          393 a          205 c          248 g          344 t
ORIGIN      
        1 aaatcaggat acaaataata gttcatttag ctacggagat aaccgattcg agaagtctca
       61 aggtagcaag tggacatttc aggaataatt gaggaggact tatggcagat tggaatggtt
      121 atattatgga tatcagcaaa caatttgatc agggcgttga tgatctgaac caacaagttg
      181 aaaaggcgtt ggaggattta gcaaccaatc cttccgaccc gaaattcctt gccgaatatc
      241 agagtgcatt agctgagtat acattatatc gaaatgcgca atctaacgtt gtaaaagcgt
      301 ataaagatct tgattcggca atcatacaaa acttccgcta aactggataa ggattttatt
      361 atgtcagtaa gcaatatgcc acctattgat agagctgagc aatcagctgc tcgtgaaata
      421 cagcaagcga aagttattga tttaaatgac cgggttctca atcttgataa cccggatgat
      481 aaaatgatct ccgcttttgc caactatgcg gtacaaactg aaaattggca gcaaaatgcg
      541 ttacaagcat taaggtctga taaaaagggg ttaactcctg aaaaattatt agtcttgcag
      601 gatcatgtgc ttaattataa tgtcgaggtt tcattggtgg gaacattagc aagaaaaatt
      661 gtcgctgcag ttgagacgtt gactcgctca tgaaatatat atcattattc ttgtttatcc
      721 tgctgttgtg cggttgcaag cagcaggaat tactgaacca tcttgatcaa cagcaagcta
      781 atgatgtcct tgctgtttta caaagacata atattaatgc tgagaagaag gatcaaggca
      841 aaactggatt ttcaatctat gtagaaccaa cggattttgc ttctgcggtg gattggctaa
      901 aaatctacaa tctcccgggt aagcctgata ttcaaatatc gcagatgttc cctgcagatg
      961 cactcgtttc ctctccacga gcggaaaaag caagattgta ttctgctatt gaacagcgtt
     1021 tagagcaatc tctcaaaatt atggatggca ttgtttcttc aagagtacat gttagttacg
     1081 atgttgatac aggtgatagt ggcaaaactg ctttaccaat tcatatttct gtacttgctg
     1141 tatatgaaaa agatatcaat ccagaaataa aaataaatga cattaagaga
//
LOCUS       DACBVI010000320         1187 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.325, whole genome shotgun sequence.
ACCESSION   DACBVI010000320 DACBVI010000000
VERSION     DACBVI010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.325"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..479)
                     /locus_tag="IU325_23045"
     CDS             complement(<1..479)
                     /locus_tag="IU325_23045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP5811849.1"
                     /translation="MIKIENLTKSYRTPAGRYYVFKDLNIDIPTGKSVAFIGRNGAGK
                     STLLRMVGGIDRPDSGRIITNKTISWPVGLAGGFQGSLTGRENVKFVVRLYSKKEDMK
                     DKIKFIEEFAELGKFFDMPIKTYSSGMRARLAFGLSMAFKFDYYIVDEVTAVGDFRF"
     gene            complement(476..1165)
                     /locus_tag="IU325_23050"
     CDS             complement(476..1165)
                     /locus_tag="IU325_23050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP5811850.1"
                     /translation="MGYLWAILEPSAHLLMLLGILGYVMHRTMPDISFPVFLLNGLIP
                     FFIFSSISKRSISAIEANQGLFNYRSVKPIDTIIARALLETLIYVIVYILLMLIVWIV
                     GEYFKITNLLRIIGTWSLLIILSCSIGLIFMVVGRTFPEMQKVLPLLNKPLYFGSCIM
                     FPLHSIPKQYWPYLLWNPLVHVVELSRESVMPGYISEGVSLNYLAMFTLVTLFISLAL
                     YRNREEAMLTS"
BASE COUNT          403 a          259 c          190 g          335 t
ORIGIN      
        1 ttaaatctaa aatccccgac cgcagtcact tcatcaacaa tataataatc aaatttaaag
       61 gccatgctta acccaaaggc taaacgcgcc ctcatgcctg atgagtacgt tttgatcggc
      121 atatcaaaga atttaccaag ttcggcgaat tcttcgataa acttaatttt atctttcata
      181 tcctccttct tggagtataa tcggacaaca aactttacat tttcacgacc ggttaaactc
      241 ccctgaaatc ctccagctaa accaacaggc catgaaattg ttttattagt gataattctt
      301 ccactatcag gtctgtcaat tccgccgacc attctcagta gtgttgattt tccggcacca
      361 tttcgaccaa taaaggcaac acttttgcct gtaggaatat ctatattcaa atctttaaag
      421 acataatatc gtcccgctgg agtgcgataa gacttcgtca aattctcaat tttaatcatg
      481 atgtaagcat tgcctcttca cgatttctgt ataatgccaa actgatgaac agagtgacta
      541 aagtgaacat tgccaagtag ttcaaactca caccttcact aatataaccg ggcataactg
      601 attccctgct taactccacc acatgtacta atgggttcca gagaagatac ggccaatatt
      661 gctttggaat cgagtgtaat gggaacataa tacatgaacc gaaatacagt ggtttattca
      721 gtaacggcaa tactttctgc atttcaggaa atgtgcgtcc tacaaccata aatattaaac
      781 caatactaca tgaaagaata atcaacagac tccaggttcc tattatcctt aaaagatttg
      841 ttattttaaa atattcgcct acaatccaaa cgatgagcat aagcagaata taaacaataa
      901 catatattaa tgtttcaagc aatgcacgtg ctattatcgt atcaataggt ttaactgagc
      961 gatagttaaa taatccttgg ttagcctcga tggcactaat tgaacgtttg ctaatgctac
     1021 taaaaataaa aaagggaatc agaccattaa gtaaaaaaac tgggaacgaa atatccggca
     1081 ttgtacggtg cataacgtat cccaaaatcc ccaacaacat cagcaaatgt gccgagggct
     1141 caagaatggc ccagagatat cccagtcgat atttaccaaa gcgcgtt
//
LOCUS       DACBVI010000321         1165 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.326, whole genome shotgun sequence.
ACCESSION   DACBVI010000321 DACBVI010000000
VERSION     DACBVI010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.326"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..643
                     /locus_tag="IU325_23055"
     CDS             <1..643
                     /locus_tag="IU325_23055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HAP5811851.1"
                     /translation="QLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWN
                     FYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKPPTPDPSKLPDIAKQLRLPYPID
                     QASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCTPQDDPQYPGMETKTASDYLPQT
                     TWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDLGEILAKMKQRTSRGIRK"
     gene            640..>1165
                     /locus_tag="IU325_23060"
     CDS             640..>1165
                     /locus_tag="IU325_23060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811852.1"
                     /translation="MKRALFFLLMIFVSFGVIANCETQAKDQDCFTIFTKGTIFSAFP
                     VLNNKTMWRWYQNEDIGEYYWQTELGICKNNKFTPSGARLLIRVGSLRLNENHATKGT
                     LQELLNTAEKTAFLGDRFRSYIRAGIYQKKSSDPAQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKDESYEC"
BASE COUNT          376 a          236 c          258 g          295 t
ORIGIN      
        1 gcagctcatc cgcctgccgg gtcagcagta tgatgaggag tccggcctgt attacaaccg
       61 ccaccgctat tatgacccgc tgcaggggcg gtatatcact caggatccga ttggactgaa
      121 ggggggatgg aatttttatc agtatccgtt aaatccggtt cagtatatag attcaatggg
      181 actggcatct aaatatggac acttaaataa tggcggatat ggagcgagac ccaacaaacc
      241 gcctacgccc gatccaagta agttgccaga catagcgaaa caattaagac tgccatatcc
      301 tattgaccag gccagtagtg cgcctaatgt tttcaaaaca ttcttcagag cattaagtcc
      361 ttacgattac acactgtatt gcaggaagtg ggtaaaaccc aatctgactt gtacgccgca
      421 ggatgatccc cagtatccag ggatggagac aaagacagca agtgattacc tgccacagac
      481 aacttggcca acaactcaat taccaccagg atatacttgt gcagaaccct atttattccc
      541 agacattaat aaacccgatg ggccagcaac agcagggata gatgatttgg gtgaaatttt
      601 agctaaaatg aaacagagaa catcgagagg aataagaaaa tgaaaagagc tttgttcttt
      661 ttgctgatga tattcgttag ttttggtgtt atagctaatt gcgaaacaca agctaaagat
      721 caagattgct ttaccatttt cactaagggt actatttttt ccgcgtttcc tgtcttaaat
      781 aataaaacca tgtggagatg gtatcagaat gaggacatag gtgagtatta ttggcaaaca
      841 gaacttggta tatgtaaaaa taacaaattc acaccaagcg gggcgagact cttaattcgc
      901 gtcggatcac tacgccttaa tgaaaatcat gcaaccaaag gaacgttgca ggagttgcta
      961 aatacagcag aaaaaacggc gtttctcggc gatcggttta gaagttatat aagagcgggt
     1021 atctatcaaa aaaagagcag tgatccagct cagttgttgg cagtccttga taattcaatc
     1081 atggttaaat attttaaaga tgagaagcca acttatgctc gaatgacagc acatctcccg
     1141 aacaaagatg agtcttacga atgtt
//
LOCUS       DACBVI010000322         1152 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.327, whole genome shotgun sequence.
ACCESSION   DACBVI010000322 DACBVI010000000
VERSION     DACBVI010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.327"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            205..1029
                     /gene="gadX"
                     /locus_tag="IU325_23065"
     CDS             205..1029
                     /gene="gadX"
                     /locus_tag="IU325_23065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HAP5811853.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQV
                     DKCVENFVLVSRDTLSLFLPMLKEEALKLHAHKKVPSLLVHNCTRDIPVFQEVAQLSQ
                     NKNLRYAEMIRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQELPNCGSAASMAAQGNFYGTDRSTEGIRL"
BASE COUNT          331 a          218 c          246 g          357 t
ORIGIN      
        1 gtcactacaa gggattattt gcttactatt aatttcactg cgttcttaag cacgcctttt
       61 gtattataaa tccagtcatc ctgcccgtag attatcatcc tgttttcccg cttcgtttaa
      121 atttatttat caatcaattt gacttaagag ggcggcgtgc tacattaata aacagtaata
      181 tgtttatgta atattaagcc aactatgcaa tcactacatg ggaattgtct aattgcgtac
      241 gcaagacata aatatattct caccatggtt aatggtgaat atcgctattt taatggcggt
      301 gatctggttt ttgcggatgc aagccaaatt caagtggata agtgtgttga gaatttcgta
      361 ttagtttcaa gagacacgct ttcattattt ctcccgatgc tcaaggagga ggcattaaaa
      421 cttcatgcac ataaaaaagt tccttcatta ctcgttcata actgtaccag agatattcct
      481 gtttttcagg aagttgcgca actatcgcag aataagaatc ttcgctatgc agaaatgata
      541 cgtaaaaggg cattaatctt tgcgttgtta tctgtttttc ttgaggatac gcagtttata
      601 ccgttacttc tgaacgtgtt gcaaccgaat atgcgaacac gagtttgtac ggtcatcaat
      661 aataatatcg cccatgagtg gacattagcc cgaatcgcta gcgaattgtt gatgagtcca
      721 agcctgttaa agaaaaaatt gcgcgaagaa ggtacgtcat attcacagtt gcttactgag
      781 tgtaggatgc agcgtgcttt acaacttatt gttatatatg gtgtttcaat taagcgagtt
      841 gcagtatcct gtggatatca cagcgtgtcg tatttcattt acgtttttcg aaattattat
      901 gggatgacgc ccacagagta tcaggagcga tcggcgcagg aattaccgaa ctgtggctcg
      961 gcggcaagta tggctgcgca agggaatttt tacggcactg accgttctac ggaaggaata
     1021 agattataga gttttactca gacataaaaa aaacccggca taggggaccg ggaagaggat
     1081 agtctgccgt ctccagacta ataaaccgtt ataacactcc ctgttggcac gggaaacttt
     1141 gtgctctcag ta
//
LOCUS       DACBVI010000323         1148 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.328, whole genome shotgun sequence.
ACCESSION   DACBVI010000323 DACBVI010000000
VERSION     DACBVI010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.328"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..784
                     /locus_tag="IU325_23070"
     CDS             <1..784
                     /locus_tag="IU325_23070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811854.1"
                     /translation="NSKKVCCIYVLFSLLAGCVSESLIDEKKKKAQTTQSNFNKNAPQ
                     QLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMRK
                     YYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIVY
                     QDTLQTGKYDTALKSTVWSDYKNEKLTNTISLRYLVRFTLVDVATGEWAAWSPVNYEY
                     KVLPPFPGKNEASTTDMTEQQIIQLKQKTYKAMVKDLVNRYQ"
     gene            complement(933..>1148)
                     /locus_tag="IU325_23075"
     CDS             complement(933..>1148)
                     /locus_tag="IU325_23075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811855.1"
                     /translation="VINFATDITIQLTLIFLLIAIVIAPLIAPFVTGYVNANYHPCGN
                     NTGIFPGAIYIKNGIKCNNEHISRKED"
BASE COUNT          385 a          210 c          237 g          316 t
ORIGIN      
        1 gaattcaaaa aaggtttgtt gtatatatgt attattctcg ctgcttgcag gatgtgtctc
       61 tgaatctctt attgatgaaa agaagaaaaa agctcaaacc acacaaagta attttaataa
      121 aaacgctccg cagcaactaa cagacaaaga cttatttggt aatgaaacca cactagccgt
      181 atccgaggag gatattcaag ctgcgcttga tggagatgag ttccgcgttc cccttaattc
      241 acctgtaata cttgttcaat ccggcaaccg cgcaccggaa accattatgc aggaggagat
      301 gcgaaaatat tatactgttt ccacattctc tggtatcccg gacagacaaa agcctctgac
      361 ttgtaacaaa aacaaagata aaaatgagaa cgaggatgtt gccagtgctg aaaatatgaa
      421 ctggatgcag gcattacgtt ttgtggcggc aaaaggccat cagaaagcga ttattgttta
      481 ccaggatacg ttgcagacag gaaaatatga cactgcactg aaatcaacag tatggtccga
      541 ttataaaaat gaaaaactga ctaacacaat ctcactgcgc tatctggtgc gattcacgct
      601 ggtggatgta gctacaggtg agtgggctgc ctggtcgccg gtgaattatg aatataaagt
      661 gcttccacca tttcccggca agaatgaagc cagtacgact gatatgacag agcagcaaat
      721 catacaactt aagcaaaaga cctataaagc gatggtaaaa gatttggtta accgctatca
      781 ataataaatt atatctgccg ccaggaattt ctggcggcaa taatacaaaa ttctttgcat
      841 aagaaattgt tcctgacaaa gacagtgcaa gaaaggacat ggaatccggc gttaaaaact
      901 gtttgtttta gaatatttaa ttatttaact gcttaatctt ccttgcgaga aatatgttca
      961 ttattacatt ttatgccatt tttaatatag attgctcctg ggaaaatccc cgtgttatta
     1021 ccgcaggggt gataattagc attgacatat cctgtgacaa aaggagctat taaaggtgct
     1081 attacgatag ctattagtaa aaatataaga gttagctgta ttgttatgtc tgtggcgaaa
     1141 ttgattac
//
LOCUS       DACBVI010000324         1130 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.329, whole genome shotgun sequence.
ACCESSION   DACBVI010000324 DACBVI010000000
VERSION     DACBVI010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.329"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..>1130)
                     /locus_tag="IU325_23080"
     CDS             complement(<1..>1130)
                     /locus_tag="IU325_23080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat family protein"
                     /protein_id="HAP5811856.1"
                     /translation="VAKLDEGHRLAALWQALPEELRLSPHRYLATNSPQGPWWVLGWC
                     ERVPEADEVLPAPLPPYRVLTGLVDRFGRTQTFHREAGGEFSGEITGVTDGAGRHFRL
                     VLTTQAQRAEEARQQASSGGTEPSVFPDTLPGYTEYGRDNGIRLSAVWLTHDPEYPEN
                     LPAAPLVRYGWTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVAHRHTGRPDIRYRYDS
                     DGRVTEQLNPAGLSYTYQYEKDRITITDSLDRREVLHTQGEGGLKRMVKKEHADGSVT
                     QSQFDAVGGLKAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHNQLTSATG
                     PDGLELRREYDESGRLIQETAHNGDITRYRYDNPHSDLPCAT"
BASE COUNT          184 a          376 c          331 g          239 t
ORIGIN      
        1 tccgttgcgc agggtaagtc actgtgtgga ttatcataac ggtagcgggt gatatcgccg
       61 ttgtgtgcgg tttcctgaat cagacggccg gattcatcat attcccggcg caattccagc
      121 ccgtcaggcc cggtggctga cgttaactgg ttgtggtggt tatagtaaaa tgccgatgcc
      181 ctgccatccg gtgtggtgat gcgcgtgatg agtcccgtca ccacatccgg gctgtactca
      241 gtcgtccggc ctgcggcatc cgtctgtgcc ttaagcccgc ccaccgcgtc aaactgactc
      301 tgcgtgacgc tgccgtccgc gtgttccttt ttcaccatcc gcttcagccc gccttcgccc
      361 tgcgtgtgca gcacttcacg gcggtccaga ctgtcggtga tggtgatgcg gtctttctca
      421 tactgatacg tgtagcttaa gcctgccggg tttagctgtt ctgtcacccg cccgtcgctg
      481 tcgtaacggt aacggatatc cggccggccc gtgtgacggt gcgccaccat ccggccccgg
      541 tatttatcat cgtaagtaaa gctgcgcacc tgtgtattgc tgcggtcata caccgccgcc
      601 agttcgccgc gtggcgtcca gccatagcgc accagcggcg cggcaggtaa attctccggg
      661 tattccgggt cgtgcgtcag ccacacggca gacagacgga tgccgttgtc ccggccatat
      721 tcggtgtaac ccggcagggt atcaggaaaa acggacggtt ccgtcccgcc ggaagaggcc
      781 tgctgccggg cttcttctgc ccgctgcgcc tgcgtggtca gtaccagccg gaagtgacgc
      841 ccggcaccat ccgtcacgcc ggtgatttcg ccgctgaatt caccgccagc ctcacggtga
      901 aacgtctgtg tgcgcccgaa gcggtccacc agcccggtca gtacccggta cggcggcagc
      961 ggcgcaggca gcacctcatc cgcctccggc acccgctcac accagccgag tacccaccac
     1021 ggcccctgcg gactgttcgt cgccagataa cgatgcggac tcaggcgaag ctcttccggc
     1081 agcgcctgcc agagtgcggc cagccggtga ccttcatcca gtttcgccac
//
LOCUS       DACBVI010000325         1052 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.330, whole genome shotgun sequence.
ACCESSION   DACBVI010000325 DACBVI010000000
VERSION     DACBVI010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.330"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..>1052
                     /locus_tag="IU325_23085"
     CDS             <1..>1052
                     /locus_tag="IU325_23085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1380-like element ISEc9 family transposase"
                     /protein_id="HAP5811857.1"
                     /translation="HIKTMLCGHFIGIDKLERLKLLQNDPLVNEFDISVKEPETVSRF
                     LGNFNFKTTQMFRDINFKVFKKLLTKSKLTSITIDIDSSVINVEGHQEGASKGYNPKK
                     LGNRCYNIQFAFCDELKAYVTGFVRSGNTYTANGAAEMIKEIVANIKSDDLEILFRMD
                     SGYFDEKIIETIESLGCKYLIKAKSYSTLTSQATNSSIVFVKGEEGRETTELYTKLVK
                     WEKDRRFVVSRVLKPEKERAQLSLLEGSEYDYFFFVTNTTLLSEKVVIYYEKRGNAEN
                     YIKEAKYDMAVGHLLLKSFWANEAVFQMMMLSYNLFLLFKFDSLDSSEYRQQIKTFRL
                     KYVFLAAKIIKTARYV"
BASE COUNT          384 a          175 c          189 g          304 t
ORIGIN      
        1 tcatataaag accatgctct gcggtcactt cattggcatt gataagttag aacgtctaaa
       61 gctacttcaa aatgatcccc tcgtcaacga gtttgatatt tccgtaaaag aacctgaaac
      121 agtgtcacgg tttctaggaa acttcaactt caagacaacc caaatgttta gagacattaa
      181 ttttaaagtc tttaaaaaac tgctcactaa aagtaaattg acatccatta cgattgatat
      241 tgatagtagt gtaattaacg tagaaggtca tcaagaaggt gcgtcaaaag gatataatcc
      301 taagaaactg ggaaaccgat gctacaatat ccaatttgca ttttgcgacg aattaaaagc
      361 atatgttacc ggatttgtaa gaagtggcaa tacttacact gcaaacggtg ctgcggaaat
      421 gatcaaagaa attgttgcta acatcaaatc agacgattta gaaattttat ttcgaatgga
      481 tagtggctac tttgatgaaa aaattatcga aacgatagaa tctcttggat gcaaatattt
      541 aattaaagcc aaaagttatt ctacactcac ctcacaagca acgaattcat caattgtatt
      601 cgttaaagga gaagaaggta gagaaactac agaactgtat acaaaattag ttaaatggga
      661 aaaagacaga agatttgtcg tatctcgcgt actgaaacca gaaaaagaaa gagcacaatt
      721 atcactttta gaaggttccg aatacgacta ctttttcttt gtaacaaata ctaccttgct
      781 ttctgaaaaa gtagttatat actatgaaaa gcgtggtaat gctgaaaact atatcaaaga
      841 agccaaatac gacatggcgg tgggtcatct cttgctaaag tcattttggg cgaatgaagc
      901 cgtgtttcaa atgatgatgc tttcatataa cctatttttg ttgttcaagt ttgattcctt
      961 ggactcttca gaatacagac agcaaataaa gacctttcgt ttgaagtatg tatttcttgc
     1021 agcaaaaata atcaaaaccg caagatatgt aa
//
LOCUS       DACBVI010000326         1045 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.331, whole genome shotgun sequence.
ACCESSION   DACBVI010000326 DACBVI010000000
VERSION     DACBVI010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.331"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            107..1045
                     /gene="tdcA"
                     /locus_tag="IU325_23090"
     CDS             107..1045
                     /gene="tdcA"
                     /locus_tag="IU325_23090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HAP5811858.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT          291 a          199 c          241 g          314 t
ORIGIN      
        1 taccatggaa agtattgaaa gttaatttgt gagtggtcgc acatatcctg ttcatttcat
       61 tttgatacac ttcatgccgt cactgaggta attaacgtag gtcgttatga gcactattct
      121 tcttccgaaa acgcagcacc tggtagtctt tcaggaagtc attagaagtg gctctatcgg
      181 ctcggctgca aaagaattag ggttaactca accggccgtc agtaaaatca ttaacgatat
      241 tgaagattat tttggcgtgg aattagtggt gcggaaaaat accggtgtaa cactaacacc
      301 tgccggtcaa ttgttactct cccgttctga atccattacc cgtgaaatga aaaatatggt
      361 taatgagata agcggtatgt cttctgaggc ggtggtggaa gtctcatttg gttttccttc
      421 attgattggt tttactttta tgtccgggat gatcaacaag ttcaaagagg tgttcccgaa
      481 agcgcaggtt tctatgtatg aagcgcaact gtcttcgttc ttaccggcaa tccgcgacgg
      541 tcgactggat tttgcgattg gtacgttaag cgcagaaatg aagcttcagg atttacatgt
      601 tgagccgctg ttcgagtccg agtttgtgct ggtagccagt aagtcccgaa catgcaccgg
      661 caccaccacg ctggagtcgt tgaagaacga acagtgggtg ttgccacaaa ccaatatggg
      721 gtactacagc gagctactta ctacgttaca aagaaatggc atcagtattg aaaacatcgt
      781 taaaaccgac tcagtcgtga caatttataa tcttgttctc aatgctgatt tcttaactgt
      841 aattccttgt gatatgacat caccttttgg ttcaaatcaa tttattacta ttccggttga
      901 agaaacatta cctgtggcac aatatgccgc ggtatggtcg aaaaattatc gtattaaaaa
      961 agcagcatcg gttttggtgg aattagccaa agagtattca tcttataatg ggtgtagacg
     1021 aaggcaatta attgaagttg gttag
//
LOCUS       DACBVI010000327         1027 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.332, whole genome shotgun sequence.
ACCESSION   DACBVI010000327 DACBVI010000000
VERSION     DACBVI010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.332"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            35..>1027
                     /locus_tag="IU325_23095"
     CDS             35..>1027
                     /locus_tag="IU325_23095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscV/YscV/HrcV family type III secretion system
                     export apparatus protein"
                     /protein_id="HAP5811859.1"
                     /translation="MFNKVLIGLRSHPELIILGLMVMIIAMLIIPLPTYLIDFLIGLN
                     LTLAILVFLGSFYVDRILSFSSFPSILLITTLFRLALAISTSRLILLEADAGEIITSF
                     GEFVIGDSLVVGFVIFSIVTIVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLR
                     AGIIDADLAKERRSVLERESQLYGSFDGAMKFIKGDAIANIIIIFVNIIGGLSVGVGQ
                     NGMDFSTALTVYTILTVGDGLVSQIPALLIAISAGFIVTRVNGDSDNMGQNIMSQLLS
                     NSFVIIVTCVLALSIGLLPGFPLLVFLCLAVILGIYFYFKFKKKGVEETVVEGDI"
BASE COUNT          268 a          168 c          231 g          360 t
ORIGIN      
        1 caaaataaaa aaccatggaa gaaggtaatg ttcaatgttt aacaaagttt tgatagggtt
       61 aaggagtcat ccagaattaa taattcttgg attaatggtg atgattatcg ccatgctcat
      121 aattccattg cccacctact taatcgattt tttgattggg ctgaacttaa cattggcaat
      181 acttgtgttt ttaggatcat tctatgttga taggatctta agtttttctt cattcccatc
      241 aattcttctc atcacaacgt tatttcgttt ggcgctggcg atcagtacca gccgacttat
      301 acttcttgag gcggacgccg gagaaattat aacgagtttt ggtgaattcg ttattggaga
      361 tagcctggta gttggttttg ttattttctc cattgttact attgttcagt ttatcgtgat
      421 tactaaaggt tcggaacgtg tggctgaagt tgctgcccgc ttttcgcttg acggtatgcc
      481 aggtaaacaa atgagtattg acgccgatct acgcgccggg atcattgatg ccgatttagc
      541 aaaagaacgt cgtagcgtat tagaacgaga gagtcagctg tatggttcct ttgatggtgc
      601 gatgaaattt atcaaaggtg atgctattgc aaacattatt attatctttg ttaatattat
      661 cggtgggcta tcagtaggag taggccaaaa tggaatggat ttctcgacag cgttgaccgt
      721 ctacacaatt cttaccgtcg gtgatggtct tgtttcacaa attccagctt tattaattgc
      781 tattagtgca gggtttatcg taacgcgcgt gaatggcgat agtgataata tggggcaaaa
      841 tataatgtcc cagttattaa gtaattcttt tgtcattatt gtgacatgtg tcctcgcgct
      901 aagtattggg ttgcttccag gttttcccct tcttgtgttt ttatgtttgg cagtaatctt
      961 aggaatttat ttttacttta aattcaaaaa gaaaggggtt gaagaaacgg ttgttgaggg
     1021 cgatatc
//
LOCUS       DACBVI010000328         1009 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.333, whole genome shotgun sequence.
ACCESSION   DACBVI010000328 DACBVI010000000
VERSION     DACBVI010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.333"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            62..982
                     /locus_tag="IU325_23100"
     CDS             62..982
                     /locus_tag="IU325_23100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP5811860.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTTLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPDAALEAGLTEIDLFEATEYYRSPRGCAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPIMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALEKLIPFYKTHL"
BASE COUNT          278 a          208 c          245 g          278 t
ORIGIN      
        1 gccagttgcg gataatagaa atgatattta aatctgttta ggcaaatccc cggagcttga
       61 aatgaaaact gtttcgataa aacatactta ctgggatata gctgctgata tctatttccc
      121 tgacgatttt aatagtgaaa aaaagtatcc tgccattatc agcgcccatc caattggaag
      181 ctgcaaagaa cagacatcgg gtagtgtgta tggtgccgcc ctggcaaaag ctggttttat
      241 cgtcattgct tttgatgcca gttttcaggg aagcagcggt ggagaaccgc gttatctgga
      301 agatccaaca atgcgagtaa aagacttcag cattgtcgtt gattacctta ccaccttacc
      361 ttacgttgat gccgggcgta ttggtgtact gggtatttgt ggtggaggtg gatatgccat
      421 taatgccgca atgacagaac gtcgaattaa agctattggt actgtcacgg gtgcaaacta
      481 cgggcgtctt atgcgtgagg gatttacagc gtttaatcct atcggagcac tcgaagcgat
      541 ggcgcaacaa agaacagacg aagctaacgg cgcaaaactt cgcgtagatg atttactccc
      601 ctcttctccg gatgcagctc ttgaagcggg attaacagaa atagaccttt ttgaagccac
      661 agaatattat cgttcgccac gaggctgtgc acctaatggg gtaaatcgtt cgctattctc
      721 acatcagact gttgccgtgg gttgggatgc atttcatctt gcagaagtcc tgttaactca
      781 accaataatg gttgtggtgg gcgatagagt gggcgcattt ggtgcctacc gtgacggctg
      841 cgaaattatt ggccgcgctg cctcaaaaca taaagagctg gtcgtggttg aaggttattc
      901 acattacgac ctgtatgata agccagaacc tgtgaaacag gcactcgaaa aattaattcc
      961 tttttataaa acacatttgt aataatctta cagggagatt ccttatctc
//
LOCUS       DACBVI010000329         1006 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.334, whole genome shotgun sequence.
ACCESSION   DACBVI010000329 DACBVI010000000
VERSION     DACBVI010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.334"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(28..954)
                     /locus_tag="IU325_23105"
     CDS             complement(28..954)
                     /locus_tag="IU325_23105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP5811861.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITRAALSQNLKS
                     LEERLNTKLIYRTTRNMSLTEEGQRLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSNVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKIES"
BASE COUNT          308 a          246 c          184 g          268 t
ORIGIN      
        1 ttcttaccga tattctgaca cacatcatta actttctatt tttactcttt tctccaccat
       61 aaaatcaata aaagcacgaa ctttagaggg cattctcacc cgtgatgaaa catatatata
      121 aaatccagga aatgaaacgg cccaatcctc aagaatcggc tgtaacttgc ccgcattgat
      181 atgttcagcg attgcaaaat ccagatgttg aatgataccc gttccattca gcgccattcg
      241 tatcatgctt tcgtcatcat tagaaatata cttacctgaa acctctattt cgaactcttc
      301 attacccccc acaacattag aaaactccca tgcagagagt gcaccgctac ttttatgccg
      361 caaccgaagg caattatggt tactcagttc ctgaggcgtt tcaggaatac cgtgttcttt
      421 taaataatct ggcgatgcca cagtaaccag tttaataagc ggagaaacag gaacggcagt
      481 catatgctca tccagtcctt gctcaagacg tatgcccaca tcacagccgt ccgcaataat
      541 gttagataaa ccgtcatcca taatgagttc tatctttgtt ttggggtaac gagtaagaaa
      601 ctcgccaata tgaggctcaa caagcatccg tgctgcaaca cgggaggaat ttattctcaa
      661 caggcctgta ggttcaagtt gggtatcacc aacatctttc aaagcatcat caatttgccc
      721 caaagccgac accagcactt catacagacg ctgtccttcc tcagtaagcg acatattgcg
      781 ggtcgtacgg tatatcagct tggtgttaag acgttcctca agagatttta aattctggga
      841 caatgcggcc cgcgtaatac ccatttcaga agcggctctg gaaaaactgc cggcacgaac
      901 aataagcgca aaccaggcaa gagaaggcaa aattgaaggc tctattttca tcattgtaaa
      961 ccttaactta acactgtgat agatattaga ccattgatga taacca
//
LOCUS       DACBVI010000330          976 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.335, whole genome shotgun sequence.
ACCESSION   DACBVI010000330 DACBVI010000000
VERSION     DACBVI010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.335"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            136..807
                     /gene="queE"
                     /locus_tag="IU325_23110"
     CDS             136..807
                     /gene="queE"
                     /locus_tag="IU325_23110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HAP5811862.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          256 a          214 c          231 g          275 t
ORIGIN      
        1 acgttactcc cttttattat taacgatctg tttctcattt ttctttaaac ctgcctgtaa
       61 cagaatgtta ataagacatg attgttgtgg caggatctgc aataattaac aattatccac
      121 ttcacagaga atgctatgca gtacccgatt aacgagatgt tccagaccct gcaaggtgag
      181 ggttacttta ccggcgtccc cgccattttt attcgtttac agggatgccc ggttggctgt
      241 gcctggtgcg acaccaaaca cacctgggaa aagcttgagg atcgggaagt ctcccttttc
      301 agcattctgg cgaagaccaa agagagtgat aagtgggggg cggcgagcag tgaagacttg
      361 ctggctgtca ttggtcgcca gggatacacc gcgcggcatg tggtgattac gggtggtgag
      421 ccttgcattc atgatttgct gccactgact gacctgctcg aaaagaacgg ttttagctgc
      481 cagatagaaa ccagcggtac tcatgaagtg cgttgtacgc cgaatacctg ggtcaccgtg
      541 tcgccaaagc taaatatgcg tggtggttat gaagtattgt cgcaagccct ggagcgagct
      601 aacgaaatca aacacccagt tggacgcgta cgtgatatcg aagcactgga tgaattgctt
      661 gcgacgctga ccgatgataa accgcgagtc attgcactgc agccgattag ccaaaaggat
      721 gatgccacac gtttgtgcat tgaaacctgc attgcgcgta attggcgttt gtcgatgcaa
      781 acacataaat atttaaatat tgcctgatta cacatttata agcgttataa atgggtggaa
      841 cctgtaaaga cttctactca tttatattct ttgtcgcctc taaaaaacct ccattttgcc
      901 catcctggac taatcattat cattttctac aaattctgtg gcgttaattt ttcgttggag
      961 tgaaaattat tacgtc
//
LOCUS       DACBVI010000331          957 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.336, whole genome shotgun sequence.
ACCESSION   DACBVI010000331 DACBVI010000000
VERSION     DACBVI010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.336"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            212..877
                     /locus_tag="IU325_23115"
     CDS             212..877
                     /locus_tag="IU325_23115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP5811863.1"
                     /translation="MTQIDFKSLSSLSPFQLTQYIDDHHFTTEQKVQIFEHYHSTHKD
                     ITNIYSRKENIKSIKKDVDYVHMFTKNFQQLAELDISKNELKVIARILNIMEFGNCFH
                     ISQAKLCRELDIKKSNMSLVFKKLKKKGIIIDKEGDLFMNSNIFLKGQAHSINAKQRP
                     NVKKAQGFVVDEKERFENVYTFRTDTDENNDVDSNSQLSTDDEVLSLQRPKIPECLFN
                     EVE"
BASE COUNT          355 a          167 c          145 g          290 t
ORIGIN      
        1 gttccgcact aaaactatta ttcaataact ggcggacatt ccactctaag taaacttatt
       61 ccagccagaa agtaataagc tttttatcca tcatactcaa tggatagctc tccactattt
      121 tataactttt tccacttatt tttgactttg ttattgacga tagtgtttaa tctgttatgt
      181 attaataaaa aacaataaaa ggaaatatac gatgacacag atagatttta aatcccttag
      241 cagtttatca ccctttcagc taacacagta tattgatgac catcatttta ctactgaaca
      301 gaaagtgcag atattcgaac attaccacag cacacataaa gatattacca atatctattc
      361 aagaaaagaa aacatcaaat ccatcaagaa agatgttgat tatgttcata tgttcacgaa
      421 aaacttccag cagctcgcag aacttgatat ctcaaagaat gagttaaaag ttatcgcaag
      481 gatcttaaat atcatggagt ttggaaactg ctttcatatc tcacaggcaa agctatgccg
      541 agagttagat attaaaaagt caaatatgtc ccttgtcttt aaaaaactca aaaagaaagg
      601 gatcattatt gataaagaag gagatctctt tatgaactcc aacatattcc ttaaaggaca
      661 agctcattct attaacgcaa aacagcgacc taatgtcaaa aaggcacagg gatttgttgt
      721 tgatgaaaag gaaagatttg agaacgttta tacttttaga acagatacgg atgaaaataa
      781 tgacgtagac agcaatagcc agctatctac ggatgatgaa gtcttgtctc tacaacgccc
      841 taagataccc gaatgtctat ttaatgaagt agaataatag atattttatc aagggtatta
      901 tattgagcgc ttcactgtaa ttttacactt aattttcaac tcgcaatata taatata
//
LOCUS       DACBVI010000332          944 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.337, whole genome shotgun sequence.
ACCESSION   DACBVI010000332 DACBVI010000000
VERSION     DACBVI010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.337"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            170..802
                     /locus_tag="IU325_23120"
     CDS             170..802
                     /locus_tag="IU325_23120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP5811864.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT          318 a          159 c          175 g          292 t
ORIGIN      
        1 ataatcatat ttaataacga gaaatgcatt tttaaatgct ttactcagaa ttatagcaaa
       61 ttcagattaa tcgcaatttt tcccagcaaa tttacaaaat catggtgtat tgacatcatc
      121 aataaccaat gaaattatgc aattatatac ggatagggag gttcttaaca tggggctttg
      181 tagtcgttat aaaagtctta catgcaatag ttgctcaatg cattgccaaa taatgccaga
      241 agagtcaccg cgtttgcagt attgcgcaaa ctcgtgtttt tgtatgtggc ccgaagaaag
      301 ttcatatttt aatcgtggtg tggtggaagg tattttaaca aaaaaccaca atgcgagatt
      361 aagcggatat atttttgtcg atttttcagt aagtttttta cgtctattcc ttgaaaaaga
      421 ctggattgac tatcttgcaa gtactgacat ggggattgtt ttagtcagcg accgcaatat
      481 gcaatcgtta gctaactact ggcgaaaaca caactctgca atatctgctg tcatatataa
      541 tgatgatggt cttgatgttg ccaatgaaaa gatcagacaa ctatttattg gtcgttattt
      601 atcatttacc agagggaaca cattaactca gatggaattc accataatgg gatatatggt
      661 ttctggttat aatccatatc agattgctga agttctggat atggatatcc gtagcatcta
      721 cgcgtacaag caacgaatcg aaaagagaat gggtggtaaa ataaacgagt tatttattcg
      781 ttcacattcg gtccaacatt gatactaaat aatctgccag aatcatactc tgcttatacg
      841 ggcatctatt gaaatgcccg tattttacaa ataagcagta accccaagag taaacaacat
      901 ggtatttaaa accatcacac gaaaaattca gaagcattac gaaa
//
LOCUS       DACBVI010000333          934 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.338, whole genome shotgun sequence.
ACCESSION   DACBVI010000333 DACBVI010000000
VERSION     DACBVI010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.338"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..469
                     /locus_tag="IU325_23125"
     CDS             <1..469
                     /locus_tag="IU325_23125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HAP5811865.1"
                     /translation="QKHSNNKDREKKYRDGDKINGPQAHSLDITNERRFADNRTMFTQ
                     HIEKQRNVHTLNQNDINNSANNANARENELTYQFQRWGQNHTVRILESSEGIRLKPSD
                     TLVSDRLHEAQHNDVTAQRWVLTEQDERQGQRHQPHDEQENEGKFENDQKDES"
     gene            429..934
                     /locus_tag="IU325_23130"
                     /pseudo
     CDS             429..934
                     /locus_tag="IU325_23130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
BASE COUNT          336 a          171 c          203 g          224 t
ORIGIN      
        1 tcaaaaacac agtaataata aagatcgtga aaaaaaatac cgcgatggag acaaaataaa
       61 tggaccgcag gcccattcat tagacattac taacgaaaga aggtttgctg ataacagaac
      121 catgtttact caacatatcg aaaagcaaag gaatgttcat acgctcaatc agaatgatat
      181 aaataattct gctaataatg ccaatgcgcg cgaaaatgaa ttgacatatc agtttcagcg
      241 ttggggacaa aatcatacag tccggattct ggaaagtagt gaagggatac gactaaaacc
      301 atcagacaca cttgtctccg acagactcca tgaagcccaa cacaatgatg ttacagcaca
      361 acgctgggta ttgactgagc aagatgaacg acaagggcaa cgacatcagc ctcatgatga
      421 gcaagaaaat gaaggtaaat tcgaaaacga tcagaaggac gaaagttaat gttcggtctg
      481 agaaaagtaa atggaaacac tcattccctt gaaaccactt ttcaaaattg gaaagaaaag
      541 ggcgaagatg ttgcgttatt aatgcccgaa tttagcgcca aatggctacc gatagcagaa
      601 gagagcggca gctggtctgg atgggtgtta cttcgagagt tatttccctt aatttctgcc
      661 gagctcgctg ggatggcttt aatgccagaa acggaaaggt taatagggga atggctcagt
      721 ttatctagta tcccgttaaa tctaaagtat cctgaactaa aatataatcg tttgtgtgtc
      781 ggtaaagtat ttgatggagt attgagtcca gctcaaccac tgataagaat atggacaggg
      841 gaattaaata tttggttaga taaagtcaca gtctgccaat acgaaaacgc tccaacgcta
      901 gacaaaaaat cattatattg gcccattcat tttg
//
LOCUS       DACBVI010000334          923 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.339, whole genome shotgun sequence.
ACCESSION   DACBVI010000334 DACBVI010000000
VERSION     DACBVI010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.339"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
BASE COUNT          269 a          178 c          154 g          322 t
ORIGIN      
        1 tagcatcatg gttttatgat cggggatgaa ttgaactact cgatcagatc taaaaacatg
       61 tggagaaagt ggattttttg catagggatt atgaaatatc atcaagccgt ccagaatgtt
      121 ttcattatat tctgaaggct tacatattat cccctttggt ttaccttctg gtggaactgc
      181 ccaaactgag ctgatttgac catttggatt atttttattc gctaatgcaa caaccttacc
      241 ccaagttgcg ttgcaattaa aaatgatggc gctaatattt tcgtactgat tatcacaaaa
      301 aaaaccgagc ggcacttctg taccattatc tttagtaata taatctaaat gttgatttgg
      361 aggcccttca ggatatagct ctggattatc taaataagca tcttcgttta cgtaatcact
      421 atatagcaca gctctgattg cacgatcata ctgcaagtta aaatatggct gttcaaatgg
      481 tgctacggcc aaaacaaatg ctttaccttt gacatgatcc aactttgaat aactgctatt
      541 atattttctg tctttttgcg ttatagcact actgagcctt ataatagctt ctctattcaa
      601 ctctccaaat cggtttaact ttaccatctc ttctggtgta taattttttt cccactcagg
      661 tgttttatcc cttgccgcat ttgccgttgt agcttcaatg acaaagtctg aatcagagat
      721 acagaaatcc ggtgtagaga aattaaagtt aacatctaag ccataatttt tcaacactgc
      781 ataaagatag acttcccaaa atgttgagtt gaatgatgtt tgaaattctt ttattatttt
      841 gttatctctg tccacaaatc cgtttgccca ttgattaaaa agagcttggg ccagttgatc
      901 attctttaat agcacccttc tga
//
LOCUS       DACBVI010000335          917 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.340, whole genome shotgun sequence.
ACCESSION   DACBVI010000335 DACBVI010000000
VERSION     DACBVI010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.340"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            1..375
                     /locus_tag="IU325_23135"
                     /pseudo
     CDS             1..375
                     /locus_tag="IU325_23135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311544.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            439..917
                     /locus_tag="IU325_23140"
                     /pseudo
     CDS             439..917
                     /locus_tag="IU325_23140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311544.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          320 a          141 c          178 g          278 t
ORIGIN      
        1 ttaaaagtat acaacgtaca ctttgataat aatcaagagt tagttgctga tggtgagcct
       61 gacgtagtat ttttaaaaaa aacggtgtgg gaagaccttc tcgttaaact aaagctggag
      121 aacaaagaaa atgcggtttc tgaaattaac ctgtcatcta ataaaaataa tgttgatcag
      181 tttattgaat gcgctaagag aaatgaacag accctattcg gcaatataag aaaaagtgat
      241 tttcatgttg cttcacttca gccaggtaga acgcgtagtg ttatttcaga aacgccgcca
      301 aatgactgta tggaatcaca taatttatat gaaaaccaca cagatacggt ttcaactgta
      361 actaaaaatt ctcagcaagt taaaggtcac tatggagata agttgaaaga aatgcagttg
      421 ttcctcaacc agatgagcaa tgcacttcaa caggattcat ctttgttaga gtcaaaggaa
      481 cacaccatag atattcaaga aaaaacgaat aagtttgtgc agcattttca gcgggtatta
      541 tttgataaaa atggaaggtc atcagagttt ctacttaatt tttatgagtg ttgctataag
      601 tttttaccaa gagcgcagcc tcaagataaa atcgatagct ataattcagc actgcaagct
      661 ttttccatct tttgttcatc tacgttgaca cataataatg tagggtttaa tttcaaatta
      721 tttccagaag tcaagctgtc tggaggagag cttgaaacgg tattcaaata caaaaatggt
      781 aattttgtct gggagatagc cagaattaaa attactctcc caaaagaaga gggtggttta
      841 tataatttac gtggattgga ttttaaggga tgcttctttt ctggacagaa cttcagtaac
      901 tatgatattc aatatgt
//
LOCUS       DACBVI010000336          913 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.341, whole genome shotgun sequence.
ACCESSION   DACBVI010000336 DACBVI010000000
VERSION     DACBVI010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.341"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            93..902
                     /locus_tag="IU325_23145"
     CDS             93..902
                     /locus_tag="IU325_23145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP5811866.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKGNAGYKESIGGANNFNNKILKHIKSYLIML
                     SIFAIGVYSAHWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVAGCLTLSYREVNRD"
BASE COUNT          305 a          172 c          172 g          264 t
ORIGIN      
        1 atataaaatt aatatatatt tatgccgtaa ataccaatat acttagcaaa ctatgtgatt
       61 tccattttga ttgatttagt gtctattgac gtatgtactg gattattaac gataatatcg
      121 agttctggcc tgagcaccga aaattaatat cggtacataa cgccgatctt aacgtcgttc
      181 tgacaacgcc agccagtcgt tgtttatcac ttctacttga agcttttcct gatgtggttg
      241 cacaacaaga ttttttcacc agagtctggg aagaagaagg tatgcgtgtg ccaactaaca
      301 cgttatatca gaacatatcc attatcagac gcggatttcg cgctgttggt gataccaccc
      361 actcgcttat tgcaaccgtg ccgagaagag gattcaagat tcataatgac attaacatac
      421 aaaatcatgt aataaactct tcaacagacg cacatacaca caatgcccca cctgccataa
      481 aaggtaatgc ggggtacaaa gagagcattg gtggcgcaaa caatttcaat aacaaaatcc
      541 tcaaacatat aaaaagttat ctaattatgt tgagcatatt tgctataggc gtatattctg
      601 cacattggct atggaataat aatcaaccga agccattctt caaggattac aagactgttg
      661 cggaaattaa tggctgccat tttaatgtga cagaagatac aattgatggg ttgaaagagt
      721 tcgataaata taagacacgg atactggatt cggggattaa ctgtaaaaaa tatccgtggt
      781 tatacttccc tcttgcaaaa tcctcaccag ggatgattgt tatggcatgc aataaaaact
      841 ataaccaaca tgaagtggca ggctgtttaa ccctgtctta ccgtgaggtt aatcgtgatt
      901 gattataaaa aaa
//
LOCUS       DACBVI010000337          909 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.342, whole genome shotgun sequence.
ACCESSION   DACBVI010000337 DACBVI010000000
VERSION     DACBVI010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.342"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            179..>909
                     /locus_tag="IU325_23150"
     CDS             179..>909
                     /locus_tag="IU325_23150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811867.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRVGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNIWAGGNAS
                     LTTITTSVIGGNENSKGTVNVLGGTWRLYDSGNNARPLNVGQSGTGMLNIKQKGHVDG
                     GYLRIGSSTGGVGTVNVEGEDSILTTELFEIGSYGTGSLNITDKGYVTSSIVAIVGYQ
                     ANSNGKVVVEKGGEWLIKNNDSSIE"
BASE COUNT          256 a          162 c          230 g          261 t
ORIGIN      
        1 aagaactatg caatttttaa tttatcaatt cgaaagtatt agcaaattag ccaaattgac
       61 accttcccac ctccaaccaa cactcaatac agacctctga caatttaata agtttcattt
      121 ttaatgtctg acgactcatc tctttcgtgg ctgtattgtt tgtatatgga gcgtttatat
      181 gaatagaatc tatcgcgtga tatggaattg caccctacag gtatttcagg cctgctcgga
      241 attgactcgc agggtaggta aaacatcaac ggttaatttg cgtaaatcct ctggactgac
      301 aacgaaattc agtagattga cgctgggtgt tttgctggca ctaagcggtt cagcgtctgg
      361 tgcgagtctg gaagttgata atgatcagat taccaatatt gatactgatg ttgcttatga
      421 tgcctacctg gttggctggt atggcactgg agtgcttaat atttgggctg gcggtaatgc
      481 ctccttaacc actattacta ccagcgtcat tggtggtaat gagaactcga agggcaccgt
      541 taatgttttg ggtggcacct ggcgattgta tgatagcgga aataatgcaa ggcctttaaa
      601 tgtgggccaa tccggaacgg ggatgctgaa tattaaacag aaggggcacg ttgacggagg
      661 atatttaagg ataggttctt cgacaggagg cgtcgggacg gtcaatgttg agggagagga
      721 ctcgattctg acgaccgaat tattcgaaat agggagttac ggtacaggct cattaaatat
      781 tacggataag ggttacgtca cgagttcaat cgtcgccatt gtaggatatc aggcgaacag
      841 caatggtaag gttgtcgttg aaaagggtgg cgagtggcta ataaaaaata atgattcctc
      901 aattgaatt
//
LOCUS       DACBVI010000338          904 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.343, whole genome shotgun sequence.
ACCESSION   DACBVI010000338 DACBVI010000000
VERSION     DACBVI010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.343"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..898)
                     /locus_tag="IU325_23155"
     CDS             complement(<1..898)
                     /locus_tag="IU325_23155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811868.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNAKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYTSDQYDLAQHQLQQINACHTNTTYTNADYSKIVAQLVSLINNIETIS
                     STPLTQQTQSILNQINNIRYEKNKNSECRIIVVANPKPDKAIITKISVEEGIPVRFSV
                     QTMFSDTNFNAEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGTNFIDFNM
                     TGQNLSGFVLTLSRFYFEDLLNINFTDANLGDTIFSHKEHPTPKLYKDEQYLDKQIEG
                     LFSTLLTINDNFLRAKAEIASTI"
BASE COUNT          244 a          131 c          177 g          352 t
ORIGIN      
        1 ttattgtcga agcaatttcc gcttttgctc gtaaaaagtt gtcattaatg gttaataatg
       61 ttgaaaataa accttcgatt tgtttgtcaa gatattgttc gtctttatat aattttgggg
      121 tagggtgttc tttatgtgaa aaaatagtgt cacctaaatt tgcgtcagtg aaatttatat
      181 taagtagatc ttcgaaataa aaccttgaca aggttagaac gaacccggac aggttctgtc
      241 ctgtcatatt gaaatcaata aagttggttc cggcaaagac tttcatcctg tttttatttt
      301 cactgtgttc aatatataac ttcgtcattt tttgatacag gctttgaata tcttttatat
      361 tagtcggtaa gtcagctcgt tgttcagcat taaaattagt atctgaaaac atggtttgga
      421 cactgaatct tactggtatt ccctcttcta cgctaatttt agttatgatt gccttgtcgg
      481 gtttaggatt agcgacaaca atgattcgac attcagaatt tttattcttt tcgtatctaa
      541 tgttattaat ctgattaagt attgattgtg tctgttgcgt aagtggtgtc gaacttatcg
      601 tttcaatgtt attaataaga cttacaagtt gagccacaat ttttgagtag tctgcatttg
      661 tatatgtagt gttcgtgtgg cacgcattaa tttgttgaag ttggtgttgc gctaaatcat
      721 attgatcaga ggtatatcct tctccagaga atgtggaatt aatgggttgt tgttcttccc
      781 aattatcgca aggccacttc ggtgtatctg gcgttttagc attatcggta tttgttgtgc
      841 agttataaat atcgccgtga tatgtattta ttatacctgg ggtaaagtca attgacataa
      901 aatt
//
LOCUS       DACBVI010000339          894 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.344, whole genome shotgun sequence.
ACCESSION   DACBVI010000339 DACBVI010000000
VERSION     DACBVI010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.344"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            247..849
                     /gene="fimB"
                     /locus_tag="IU325_23160"
     CDS             247..849
                     /gene="fimB"
                     /locus_tag="IU325_23160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimB"
                     /protein_id="HAP5811869.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          262 a          165 c          199 g          268 t
ORIGIN      
        1 tattattaga ttcgtatcag attgataaat atataaaggt acatagcatg caagagcatg
       61 gcgtttgtat ggcaaagtta ttataagtaa ctatcgctac tccatttaag ttcgctcaga
      121 agaactggtc cacttacgtt agttattaag caaacgttcg cttttataaa cataatcagg
      181 attaaaatgt tggattattg ctaacccagc acagctagtg cgcgtctgta attataaggg
      241 aaaacgatga agaataaggc tgataacaaa aaaaggaact tcctgaccca tagtgaaatc
      301 gaatcactcc ttaaagcagc aaatactggg cctcatgcag cacgtaatta ttgtctgact
      361 ttgctttgtt ttattcatgg tttccgggcg agtgaaattt gtcgattgag gatttcggat
      421 attgatctta aggcaaagtg tatatatatc catcgattaa aaaaaggctt ttcaacaacg
      481 cacccgctat tgaacaaaga agttcaggct ttaaaaaact ggttgagtat ccgtacttcg
      541 tacccgcatg ctgagagcga gtgggtattt ttatcgcgta aggggaatcc gctttctcgg
      601 caacagtttt accatattat ctcgacttcc ggtggtaatg ccgggttgtc actggagatt
      661 catccgcaca tgttacgcca ttcgtgtggt tttgctttgg cgaatatggg aatagatacg
      721 cgacttatcc aggattatct tgggcatcgc aatattcgtc atactgtctg gtataccgcc
      781 agcaatgcag ggcgttttta cggcatctgg gatagagcca gaggacgaca gcgtcacgct
      841 gttttataga gatagttgag ataccaggga tggtgttaaa aactacatta ttct
//
LOCUS       DACBVI010000340          880 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.345, whole genome shotgun sequence.
ACCESSION   DACBVI010000340 DACBVI010000000
VERSION     DACBVI010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.345"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            130..>880
                     /locus_tag="IU325_23165"
     CDS             130..>880
                     /locus_tag="IU325_23165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP5811870.1"
                     /translation="MNISVIICCYNSENRIVNTLFHLSQCVSLSKGLIDEIILVDNNS
                     SDETVKTAEDFWRNTGEEQIFKIVFESIPGLVHARRRGLDESKNEIVVFCDDDNWLVP
                     SYFTKLEKYFENKQVGAVGGRGIPIANTVIPEWFFENLDAYACSAQTSNMLYGASLAI
                     RSSILKKFYTDALSVKLKGRTKDSLDSGEDNLLCNFVKKQGYTLIPDNTPFCHFMESK
                     RLDVDYLYKLAESFGKATASMKNNSFTTDYVY"
BASE COUNT          288 a          134 c          175 g          283 t
ORIGIN      
        1 tttttttcta aaagtaaaag aaatgaaata agaataaaat tgcatagaaa tggatggcgt
       61 gagtcgatac ttaaatcctc taaagtatcg ctgagcgcaa aattatgttc acttttaatt
      121 agataaaata tgaatatttc agtaataatt tgttgctata actcagaaaa tagaatcgta
      181 aacacccttt ttcatctttc acaatgtgta tctctatcca aggggttaat tgacgagatt
      241 attcttgttg acaataactc atctgatgaa actgttaaaa cagctgagga tttttggcgt
      301 aatacggggg aggagcaaat ctttaaaatt gtttttgaat cgattccggg ccttgttcat
      361 gctagacggc gagggctgga cgagtccaaa aatgagattg ttgttttttg tgatgatgat
      421 aattggctgg taccatctta ttttacaaaa ctagagaaat attttgaaaa caaacaagta
      481 ggcgctgtcg ggggacgcgg tattcctatt gctaataccg ttattcctga gtggtttttt
      541 gagaatctgg atgcatatgc atgttctgca caaacttcta acatgttgta tggtgctagc
      601 ttggctattc ggtctagtat cttaaaaaaa ttttatacgg atgctttatc agtaaagcta
      661 aagggtagaa caaaagacag tctggattct ggggaagata acttattgtg taattttgtc
      721 aaaaaacaag gatacactct gatacctgac aatacaccat tttgccattt catggaaagc
      781 aagcgattgg atgtcgatta tctatataaa ctggctgaat catttggaaa agcaacggca
      841 tcaatgaaaa acaattcctt caccaccgat tatgtatatt
//
LOCUS       DACBVI010000341          878 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.346, whole genome shotgun sequence.
ACCESSION   DACBVI010000341 DACBVI010000000
VERSION     DACBVI010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.346"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
BASE COUNT          246 a          183 c          143 g          306 t
ORIGIN      
        1 atagcagggt ttcttcatca gtcctgccat acctttcaac taaaagcttg acactatcgg
       61 taataaaatt atttttattt agatattgtg cgataaaagc tgtatttttc gcaagtgatt
      121 tattccagtt agcattcatg catttcattg tttccttcga agttgtctcc aaaaaaactc
      181 tcagcatgac aatgcagctt ataggttcat ctaatgaact caaattgcac aattctacaa
      241 aaatatgata ttccttattg cgatacttcg gaacatcgac tgtgtttcgc ccccaatcaa
      301 caaagatatc tctgtttcgc ggtagatcag tgacaggccg agctttgggc ctaacgggtt
      361 taattggctt tattggtctt actgcagcag gtccggtgtt agcaccagca ttagttactt
      421 tatcgcctcc ttcgccaccg ggaatgcctt tattatcacc tttcactggg ttttgaggtg
      481 tatctttttt cttgttataa tatcgttgtt ttatgatcga tctataatta tcagcaatag
      541 gctgtaagcg atctctcaca gcaagaaaaa gagaactatc agtacgtaat ccagttttag
      601 ctgtattcca aggtagcaaa gccgcgtctt ttgatacaaa ttttacccaa caaacataac
      661 cgttatattc tgagtgccac ttcgttttcc agccatgcga cttctctgta ctcgcagata
      721 ctataactct gtcattgcag gaaaagtata taccaaaata atctgttaat tttctattat
      781 ttgcaagact ataattatct tcaccgggga atgtatattc attatgaatc cctgtttcga
      841 taataacatc tactccatca aattttatta cttcttgt
//
LOCUS       DACBVI010000342          878 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.347, whole genome shotgun sequence.
ACCESSION   DACBVI010000342 DACBVI010000000
VERSION     DACBVI010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.347"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            62..298
                     /locus_tag="IU325_23170"
     CDS             62..298
                     /locus_tag="IU325_23170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HAP5811871.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            341..613
                     /locus_tag="IU325_23175"
     CDS             341..613
                     /locus_tag="IU325_23175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HAP5811872.1"
                     /translation="MKTSDNESTKYEITGQAVLHILRMKINFSLQTLIKQLLVMKSAE
                     ENAFRRDLIDSIIRDFSNSDTGEPNRRTAIADNKSMFNGKKINRIH"
     gene            642..>878
                     /gene="ariR"
                     /locus_tag="IU325_23180"
     CDS             642..>878
                     /gene="ariR"
                     /locus_tag="IU325_23180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HAP5811873.1"
                     /translation="MLEDTTIHNAITDKALSSYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEI"
BASE COUNT          309 a          142 c          180 g          247 t
ORIGIN      
        1 gtacaagttt gctattgtta cctcacttaa cattgattaa catttttaac agaggcgtag
       61 catgcatcaa aattcagtga ctttagattc tgcgggagca atcactcgtt actttgcaaa
      121 agctaacttg cctactcagc aggaaactct cggggaaatt gtgactgaga ttttgaaaga
      181 tggacgtaat ctgagtcgaa agtcgctttg tgccaaactt ctatgccgac tggaacaagc
      241 gacaggggaa gaggaacaga aacattataa tgcactaatt gggttgcttt ttgaataatg
      301 caacagcctg attatttaac aggttagcta tgaagtcgtt atgaagacat ctgataatga
      361 aagtacaaaa tatgaaatta ctggccaggc ggtgctccat atactgcgca tgaagataaa
      421 tttttcgtta cagactctta ttaagcaatt actcgtaatg aaatcggctg aggaaaatgc
      481 tttccgacga gatttaattg acagcataat tcgtgatttt agcaacagtg atacaggaga
      541 gccaaacaga agaacagcga tagctgacaa taaaagtatg ttcaatggta agaaaataaa
      601 cagaatacac taaaatttca taagtaagat gagaggttac catgcttgaa gatactacaa
      661 ttcataatgc aataactgat aaagcgttat caagctactt tcgtagttca ggtaatttgt
      721 tagaagaaga gtcagctgtg ttagggcagg ctgtgaccaa tttaatgctt tcaggcgata
      781 atgttaataa taaaaatatt atcttaagtc tgatacactc tctggaaaca acaagtgata
      841 ttctcaaagc tgatgtgatt agaaaaacac tggaaatc
//
LOCUS       DACBVI010000343          863 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.348, whole genome shotgun sequence.
ACCESSION   DACBVI010000343 DACBVI010000000
VERSION     DACBVI010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.348"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            75..575
                     /locus_tag="IU325_23185"
     CDS             75..575
                     /locus_tag="IU325_23185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HAP5811874.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLKPNTEPVNTLLSNNFIRINKNITLSDLTHFLVSNLHTQNVYSTQEPLTHDEID
                     ILRLCVSYSLKQIAIIKGIDYKTVSYHKIRALNKLNIKGTVELFIALCEWDKHYFKLQ
                     SCIRES"
     gene            577..>863
                     /locus_tag="IU325_23190"
     CDS             577..>863
                     /locus_tag="IU325_23190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HAP5811875.1"
                     /translation="MSLVRYNSKIIITVALISLLSACTRENSLKDTQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLVISQDRLT"
BASE COUNT          299 a          152 c          145 g          267 t
ORIGIN      
        1 atataacaat taccattatt ttaaatttta cacgtaatat ttaccctaaa tgattttaga
       61 gtgcaggttg gcatatgcaa gtcttttcca gtgatgtcta cttcactgta ggcactaatg
      121 cattattagc atcccaaaag gaatattaca gcgatttggt tgcgttggtt gatcttggac
      181 attcgttcgt tgttattgat gagcaccaac atcgaaatct caaaccaaat acagaacctg
      241 taaacaccct tttgagcaac aatttcatca ggatcaataa gaatattaca ttatctgatt
      301 taacgcattt cctggtttcg aatcttcata cacaaaatgt gtattccact caagaaccac
      361 ttacccatga tgagatagat atcctgcggt tatgtgtttc ttattcgtta aaacaaatag
      421 ccattatcaa aggaattgat tataaaacag tatcatatca caaaataaga gccctaaata
      481 aacttaacat aaaaggtact gttgagttgt ttatagcact ctgtgaatgg gataaacatt
      541 attttaaact ccaatcttgc ataagagaaa gttgatatgt cattagtaag atataatagt
      601 aaaatcataa taactgtagc gctgatatcg ttgctctctg catgcaccag agaaaattca
      661 ttaaaggata ctcagaataa aagaagtggc gaatctcaag cgatagtaga tgcaagacag
      721 ttctttagcg aacacccgga atatatatca tcagcagaac tagagcagaa gctatatcgc
      781 gaatttgcgc aggttactac tgttccagct tataaagaga tgagtatgta tcaattgctt
      841 gtcatttcgc aagatcgttt aac
//
LOCUS       DACBVI010000344          830 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.349, whole genome shotgun sequence.
ACCESSION   DACBVI010000344 DACBVI010000000
VERSION     DACBVI010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.349"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            203..278
                     /locus_tag="IU325_23195"
     tRNA            203..278
                     /locus_tag="IU325_23195"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:236..238,aa:Ile,seq:cat)
BASE COUNT          246 a          155 c          186 g          243 t
ORIGIN      
        1 gatggtaact ataaatagtg ctaagtatta gcatttgctt gttgactgag agtatcgact
       61 ttgtgaatgg aagcggtgtc gggttttatg tcgcaggtaa tgacgaccca ggatgaatga
      121 gaacgcataa aaatctgtta tttacatcat aagatcatca tgtttattgc gtggtttata
      181 agtattgtta ttgcatactc aaggcccttt agctcagtgg ttagagcaga cgactcataa
      241 tcgtccggtc gctggttcaa gtccagcaag ggccaccaaa tcatcattca ggtatcagga
      301 agagttaagg gtacaagggg tactcgatgg cggtctatat ttcacgacta acgccatttc
      361 aaggaagaag tgtggtgggt ggtttcgaat gagggctggt gttttggtac gtggcacgcc
      421 actgcaatgt atttatccat cagtactcat tcaaaagtac ctggaaaaaa tgcccgctat
      481 gccaagcggg caaattgctt acatgtggaa ttaagtaatt aacgataaag ccagagtatt
      541 atcatttatc atgcctcttc atttaatcag gttaattact caaatcactg aactattctc
      601 tgttcgctca ctgatgataa gtgaagtgtc acaaccacga tttgtatgtg gaccaatgct
      661 gtagagtaag gatgaaatgt ctctgccacg ccttaaacat ctactactga ctagaaccgc
      721 cgtcgaatgc agaagagtct tgtgagcatt acaggagtct ggctttggtc attaatcaac
      781 gctatttaga tataaatttc taacactcaa aaaacgattc tcacgaataa
//
LOCUS       DACBVI010000345          814 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.350, whole genome shotgun sequence.
ACCESSION   DACBVI010000345 DACBVI010000000
VERSION     DACBVI010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.350"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            89..805
                     /locus_tag="IU325_23200"
     CDS             89..805
                     /locus_tag="IU325_23200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP5811876.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          270 a          139 c          170 g          235 t
ORIGIN      
        1 atttttcctg ttatgatata atatgccgaa ttaacacatg ttaacgattt accagtaatg
       61 taaataaatt ttcgaggaga tcattccagt gggacgtaaa tgggccaata ttgttgctaa
      121 aaaaacggct aaagacggtg caacgtctaa aatttatgca aaattcggtg tagaaatcta
      181 tgctgctgct aaacaaggtg aacccgatcc agaattaaac acatctttaa aattcgttat
      241 tgaacgtgca aagcaggcac aagttccaaa acacgttatt gataaagcaa ttgataaagc
      301 caaaggcggc ggagatgaaa cgttcgtgca gggacgttat gaaggctttg gtcctaatgg
      361 ctcaatgatt atcgccgaga cattgacttc aaatgttaac cgtacgattg ctaacgttcg
      421 cacaattttc aataaaaaag gcggcaatat cggagcggca ggttctgtca gctatatgtt
      481 tgacaatacg ggtgtgattg tatttaaagg aacagaccct gaccatattt ttgaaatttt
      541 acttgaagct gaagttgatg ttcgtgatgt gactgaagaa gaaggtaaca ttgttattta
      601 tactgaacct actgaccttc ataaaggaat cgcggctcta aaagcagctg gaatcactga
      661 gttctcaaca acagaattag aaatgattgc tcaatctgaa gttgagcttt ccccagaaga
      721 tttagaaatc tttgaagggc ttgttgatgc ccttgaagat gacgacgatg ttcaaaaagt
      781 ttatcataac gtcgcaaatc tctaattata tttt
//
LOCUS       DACBVI010000346          798 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.351, whole genome shotgun sequence.
ACCESSION   DACBVI010000346 DACBVI010000000
VERSION     DACBVI010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.351"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            103..>798
                     /gene="ygeH"
                     /locus_tag="IU325_23205"
     CDS             103..>798
                     /gene="ygeH"
                     /locus_tag="IU325_23205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HAP5811877.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQIYIPPKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQNISNKKIDGLYTYPMAA
                     TNFCNDHISQNSFLRRFKPDYFVTGRINQNNAVKTLYIELIDAKNLFLIASNHLPVDE
                     LHNTSQFIIDNILQ"
BASE COUNT          279 a          141 c          129 g          249 t
ORIGIN      
        1 atgtaaactc ctgtagcatt aatgacacat aaatatgaat agccacaatt tctattgctt
       61 atgcaggatg caagaaacca attttttcat agaggataac taatggactt agaaaataaa
      121 ttctcatatc attttctcga gggattaacg ctcacggaag atggaattct tactcaagga
      181 aatgagcaga tttatattcc accgaaagag ttaggagtat tgatcgtatt acttgaatcc
      241 gctggtcatg ttgtcctgaa agatatgatc atcgaatcag tatggaaaaa tattattgta
      301 agtgacgagt ccctgacaag atgtatctat tctttgcgct gcatttttga aaaaattggc
      361 tatgatcgtt gcatagaaac aatctaccgg aaaggttatc gtttcagtgg acaggttttc
      421 aaaactaaaa taaatgaaga taacacttca gactattcca tagctatatt tcctttcacc
      481 acttcattga atactctgga tccattaata cttaatcagg aattagtgca aaacatttcg
      541 aataaaaaaa tcgatggcct ttatacctat ccgatggctg cgacaaattt ttgtaatgat
      601 cacatatcgc aaaattcatt tttgagaaga ttcaagccag attatttcgt tacaggaaga
      661 ataaaccaga ataacgcggt gaaaacttta tacattgaac tgatcgacgc taaaaatctt
      721 ttcctcatcg ccagtaatca tcttcctgtt gatgaactac ataacacatc acaatttatt
      781 atagataata tccttcaa
//
LOCUS       DACBVI010000347          792 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.352, whole genome shotgun sequence.
ACCESSION   DACBVI010000347 DACBVI010000000
VERSION     DACBVI010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.352"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..750)
                     /locus_tag="IU325_23210"
     CDS             complement(<1..750)
                     /locus_tag="IU325_23210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP5811878.1"
                     /translation="MLQAERHKMICAHVQKQGAVRVTELSRLFKASPETIRRDLTVLE
                     RNKKLIRSFGGAVALDSSDSAPVMLADNAWQDNSIDRAESFRKRTEENPDIKARIAKA
                     ALQFIHPGDCILMDNSSTCWFLARQIPDIDVTVVTNSLRIIQALACRDRVRIIGIGGE
                     YSERHDDFHGPVAEGAIRNFQINSFFFSCQGLNLGNGVRDGSEINARLKQVMLQVSGQ
                     KILLVDSSKFEQYAFSKICTLDEIDILVTNVC"
BASE COUNT          233 a          195 c          138 g          226 t
ORIGIN      
        1 acaaacatta gtcaccagaa tatcgatttc atccagcgtg catattttac taaatgcata
       61 ctgttcaaac ttactggagt cgaccagcaa tattttttgt cctgacacct gaagcatcac
      121 ctgtttaagt ctggcattaa tttcactgcc atcgcggaca ccattcccca gattcaaccc
      181 ctggcatgaa aagaaaaaac tgttgatttg aaaatttcgg atcgccccct ctgcaaccgg
      241 gccatgaaaa tcatcatgtc gctcagaata ctcaccaccg ataccaataa ttcgaactcg
      301 atcacggcaa gccaacgcct gaattatccg taatgaattt gtaactacag taacatctat
      361 atcgggaatt tgacgagcta aaaaccaaca agtactactg ttatccatta aaatacaatc
      421 accagggtga ataaattgca atgcggcctt cgcaattctg gcttttatat caggattttc
      481 ttcagtccgt ttacggaatg attccgcacg atcgatacta ttatcttgcc acgcattatc
      541 ggcgagcata accggagcgc tatcagatga atccagagcg accgcgccac caaagctacg
      601 gattaacttt ttgttgcgct ccagtaccgt taaatcccga cgaattgtct caggagacgc
      661 tttaaataat cgtgataatt ctgttacccg tacagcacct tgtttttgta catgtgcgca
      721 tatcatttta tggcgctctg cctgcaacat agtatcggtc cgttattatc aaattagcat
      781 gcttatataa tt
//
LOCUS       DACBVI010000348          790 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.353, whole genome shotgun sequence.
ACCESSION   DACBVI010000348 DACBVI010000000
VERSION     DACBVI010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.353"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            578..>790
                     /locus_tag="IU325_23215"
     CDS             578..>790
                     /locus_tag="IU325_23215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811879.1"
                     /translation="MADKAILWALISASTQEGRKACSLSYFSCKAAEAELGLAYMAAN
                     DNKAFLTSLSRIMMYKIDAGLSESYTC"
BASE COUNT          276 a          131 c          164 g          219 t
ORIGIN      
        1 gggtggtgtc atatggcgtt caagataatg tcatatgctc taataataaa attaatgaag
       61 attccataga aaaaatttta ttagaaggga atattgactt ttggaaaaaa ctgaaaatta
      121 agtgaaatga acgaaagaga aaattaacaa tgagcggaaa accagcggta tactaaggcg
      181 atgccatgtt gaaagatagt ccaatagtct aaagtggaga actcaatgaa gcaaaccgga
      241 cgaggcttgc ttaacagtcc tggagcaacc aatagaaaac ctaatggtat acgtttagta
      301 ccgtcagcaa atacagaaac gtataatcgt gatggatttt taacacatag ttgtttaaac
      361 gcttttggtc catctttagg acctcgattc tgttccgagg gttgtataac tggttcatct
      421 aatgacatgc aaaaattaaa tgagctaata ttttcagagc cagatagcac attaacagtg
      481 acggactaga aatatgacaa ggttattaac aattgtgctt ttcttctcta tttctgcgaa
      541 tgcagttact gtaaaccaat ttcagagaga gttggatttg gcagacaagg caatattatg
      601 ggcactaata tcagccagta cccaagaggg acgaaaagcg tgttcactta gctatttttc
      661 atgtaaagcc gcagaggctg aactcggact ggcatatatg gctgctaatg ataacaaggc
      721 atttctcaca tccttatcta gaataatgat gtacaaaata gatgctggac tttccgaatc
      781 ctatacatgc
//
LOCUS       DACBVI010000349          788 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.354, whole genome shotgun sequence.
ACCESSION   DACBVI010000349 DACBVI010000000
VERSION     DACBVI010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.354"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..>788
                     /locus_tag="IU325_23220"
     CDS             <1..>788
                     /locus_tag="IU325_23220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811880.1"
                     /translation="LNDFSHRLKMEGEEQLCDCVNELKLGLSSNNKYDLFKNKETSLW
                     KRKVPESAALAMTGTVYKLFGSRLLNARNQLDKNLLEMAMQMVYGKDMVTKSGDILID
                     IQLNKDGSLQSKHYTFDVGEVINSYNIDLFVNASSINQTYLHDKNPGDVISLYGTLDN
                     IPLVVKECTGKNHQINVKAKLPQERSEKVEGMCERMRGGISMFNHTTKTAGNIEHNLQ
                     LSFLVDSRPSITNKYSAKDVGEDILALPVNVYHADFKIKINNAV"
BASE COUNT          281 a          130 c          160 g          217 t
ORIGIN      
        1 tattaaatga tttttcacat cgccttaaaa tggaaggtga ggagcaactg tgcgattgtg
       61 ttaacgaact taaactcggg ttgtcgagta ataataaata tgacctgttt aaaaataaag
      121 aaacctcact gtggaaacgc aaagtaccag aaagtgcagc gctggcaatg acgggaacag
      181 tatataaatt atttggtagt cgcttactca acgcccgcaa tcagttagat aaaaatctcc
      241 tcgaaatggc tatgcaaatg gtttatggca aagatatggt gacaaagtcc ggggatatat
      301 tgattgatat acaattaaac aaggatggca gcctccagag taagcactac acatttgatg
      361 taggtgaagt cataaacagt tataatattg acctctttgt gaatgcttcg agtattaatc
      421 aaacttactt acatgataaa aatcctggtg acgttattag cctgtatggc actttagata
      481 acattcctct tgtagttaag gaatgcacgg gtaaaaatca tcaaattaac gttaaagcca
      541 aattgcctca agaacgaagt gaaaaagttg agggtatgtg tgaaagaatg agaggtggca
      601 tctctatgtt taatcacact acaaaaacag ctggtaatat agagcataat ttgcagctaa
      661 gttttctcgt ggattcacga ccatcgatca caaataaata ctcagccaaa gatgtagggg
      721 aagatatact tgcattgccc gtcaacgtat atcatgcaga ttttaaaatt aaaatcaata
      781 acgcagta
//
LOCUS       DACBVI010000350          786 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.355, whole genome shotgun sequence.
ACCESSION   DACBVI010000350 DACBVI010000000
VERSION     DACBVI010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.355"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            28..210
                     /locus_tag="IU325_23225"
     CDS             28..210
                     /locus_tag="IU325_23225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811881.1"
                     /translation="MIDGKIPNISKEILHLKDETERLEKEYQQENTLNDVVQNIRSWL
                     KENENMVKAIKKTDTE"
BASE COUNT          283 a          124 c          153 g          226 t
ORIGIN      
        1 attaatgaga ttaaggggat aataaaggtg atagatggaa aaataccaaa tataagtaaa
       61 gagattctac atcttaagga tgaaacagag agattggaga aagagtatca acaagaaaat
      121 acacttaatg atgtagttca aaatatcagg agttggctaa aagaaaatga aaatatggtg
      181 aaagctatta agaaaactga tactgaataa catggcatat aaataaacgg atcaagtcgt
      241 tcctgtgata atgaggtcat gcgaaaagac gcttactcat cagtaatctg gtgtacttta
      301 attaaaccag tgtagttgat gtgtaagcgt tgatgttggg agacgattaa atggttatat
      361 cactactacg ccctttccag acggcgtata agagtgcaac atgttgagtt ttccgagtga
      421 atggtgcatt ttactaaccg agccatttga atcaaccaac gcaataaaaa agactggaat
      481 aataagcctg tatttattat tcatcgcaat cgcactctac ggttttatcc tgcagaaatg
      541 agactacttc cagcggttgt ggattatccc tgggcgatag tcatagtctt gtgtcaggat
      601 tcacaaatga cctgttacct gacatggata taaacacaac atagagtgcg gtagttaatc
      661 tgaatcatct atttttcggc tacacagcac aaaacaacat tattgattct ttataagaga
      721 gaatctctcc attattttta tataaatcat tattaagaaa atccagaagc tcttgcctta
      781 cactat
//
LOCUS       DACBVI010000351          779 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.356, whole genome shotgun sequence.
ACCESSION   DACBVI010000351 DACBVI010000000
VERSION     DACBVI010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.356"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..701
                     /gene="ydeO"
                     /locus_tag="IU325_23230"
     CDS             <1..701
                     /gene="ydeO"
                     /locus_tag="IU325_23230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HAP5811882.1"
                     /translation="DYAFSDGEILMVDNAVRTHFEPYERHFKEIGFNENTIKKYLQCT
                     NIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIFAACKG
                     FITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQEQTTFSQI
                     LLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEYRCQRH
                     TGMNKGNTMNALAI"
BASE COUNT          240 a          155 c          159 g          225 t
ORIGIN      
        1 aagattacgc cttctctgac ggcgagatcc tgatggtaga taacgctgtt cgtacgcatt
       61 ttgaacctta tgagcggcat ttcaaagaaa tcggatttaa tgaaaatacc attaaaaaat
      121 atctacaatg cactaacatc cagacagtga cggtgcctgt tcctgcgaag tttttacgtg
      181 cttcaaatgt accgactgga ttgcttaatg aaatgattgc ttatctcaac tcggaagaac
      241 gcaatcatca taatttttca gaacttttgc ttttttcttg cctgtctatt tttgccgcat
      301 gcaaaggttt cattacacta ttaactaacg gtgtgctatc cgtttctggg aaagtgagaa
      361 atattgtcaa catgaagctg gcgcacccat ggaagctgaa agatatttgt gactgcctgt
      421 acattagtga aagcctgttg aagaaaaaac taaagcaaga gcaaacgaca ttctcacaga
      481 ttcttttaga tgccagaatg cagcacgcaa aaaatttgat acgcgtagaa ggttcagtca
      541 ataaaattgc cgaacaatgt ggttatgcga gtacatctta ttttatttat gcgttccgca
      601 aacattttgg caacagtcca aagagagttt ctaaggagta ccgttgtcaa aggcacacgg
      661 gtatgaataa gggcaacacg atgaatgctt tagctatttg attatttgct aacgagtagt
      721 caaccacgca ccctacgtaa aaattaaaat ggggcagcca ttccctgccc cgcgttgtt
//
LOCUS       DACBVI010000352          778 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.357, whole genome shotgun sequence.
ACCESSION   DACBVI010000352 DACBVI010000000
VERSION     DACBVI010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.357"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(32..736)
                     /locus_tag="IU325_23235"
     CDS             complement(32..736)
                     /locus_tag="IU325_23235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HAP5811883.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
BASE COUNT          178 a          208 c          200 g          192 t
ORIGIN      
        1 atgaggcagc cttttgtctt attcaaaggc cttacatttc aaaaactctg cttaccaggc
       61 gcatttcgcc caggggatca ccataataaa atgctgaggc ctggcctttg cgtagtgcac
      121 gcatcacctc aatacctttg atggtggcgt aagccgtctt catggattta aatcccagcg
      181 tggcgccgat tatccgtttc agtttgccat gatcgcattc aatcacgttg ttccggtact
      241 taatctgtcg gtgttcaacg tcagacgggc accggccttc gcgtttgagc agagcaagcg
      301 cgcgaccata ggcgggcgct ttatccgtgt tgatgaatcg cgggatctgc cacttcttca
      361 cgttgttgag gattttaccc agaaaccggt atgcagcttt gctgttacga cgggaggaga
      421 gataaaaatc gacagtgcgg ccccggctgt cgacggcccg gtacagatac gcccagcggc
      481 cattgacctt cacgtaggtt tcatccatgt gccacgggca aagatcggaa gggttacgcc
      541 agtaccagcg cagccgtttt tccatttcag gcgcataacg ctgaacccag cggtaaatcg
      601 tggagtgatc gacattcact ccgcgttcag ccagcatctc ctgcagctca cggtaactga
      661 tgccgtattt gcagtaccag cgtacggccc acagaatgat gtcacgctga aaatgccggc
      721 ctttgaatgg gttcatgtgc agctccatca gcaaaagggg atgataagtt tatcacca
//
LOCUS       DACBVI010000353          771 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.359, whole genome shotgun sequence.
ACCESSION   DACBVI010000353 DACBVI010000000
VERSION     DACBVI010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.359"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(85..366)
                     /locus_tag="IU325_23240"
                     /pseudo
     CDS             complement(85..366)
                     /locus_tag="IU325_23240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608996.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          242 a          189 c          126 g          214 t
ORIGIN      
        1 aatctaatat ttagatatag ccaatgaact aaaaaatcaa ttcccgaaag aagaatacac
       61 aaacaatatg ataaactacg ttctgatgcc gagaccatca tcatacatct ccccaagcag
      121 aaattgcgcg tcgacatcat tttgcgcagc cgctttttcg taccatgatt ttgattactg
      181 atagtcaata tccagtattt ctctatataa atacataaga cccaaggcat attgagcttc
      241 tacatctcct ttttcgcctg ctgatttcaa cttagaaaac tgagagtccc tttgaatggt
      301 cgtaatgtaa acaatcgaaa gaagtataat aaatcctgct attagccaat attttatttt
      361 tcccatgtca cacctagtcc atttaaatac tatatagcct ctacagagat ctttactcca
      421 gactgtccca gtctgggaga ttgttaacta cctttcgcca ccaatgatac gcagccataa
      481 gaacaccaaa gcaaatacct gcgactatgc ttaagacaaa tgctgcgccc ggttgcatcc
      541 cttcgtcctt ccaagtaaaa aaccacatac agatccccca tgccggacca aaccataccc
      601 ccatcagcaa ggttatttgc cagaatgagg caaaaggtag cggtggtatc ttgaatccag
      661 ctttccatgc gaggcgtagc aagggagggg cataattaca acgccacatc ttcttgcttt
      721 ccataatggc aatggagtgt tcctttttag cttcaaagct catacagttt c
//
LOCUS       DACBVI010000354          767 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.360, whole genome shotgun sequence.
ACCESSION   DACBVI010000354 DACBVI010000000
VERSION     DACBVI010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.360"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            142..738
                     /gene="fimE"
                     /locus_tag="IU325_23245"
     CDS             142..738
                     /gene="fimE"
                     /locus_tag="IU325_23245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimE"
                     /protein_id="HAP5811884.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEVRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          204 a          147 c          187 g          229 t
ORIGIN      
        1 ttgtatgata tttaatgtaa ttgactgata gccacatcac ttcgtgtgtg gttatctttt
       61 tatctattgg gctaattttg accgattgag gtttcctata ggtattcatt caaatatatc
      121 tcagtcagga gtactactat tgtgagtaaa cgtcgttatc ttaccggtaa agaagttcag
      181 gccatgatgc aggcggtgtg ttacggggca acgggagcca gagattattg tcttattctg
      241 ttggcatatc ggcatgggat gcgtattagt gaactgcttg atctgcatta tcaggacctt
      301 gaccttaatg aagttcgaat aaatattcgc cgactgaaga acggattttc taccgttcac
      361 ccgttacgtt ttgatgagcg tgaagccgtg gaacgctgga cccaggaacg tgctaactgg
      421 aaaggcgctg accggaccga cgctatattt atttctcgcc gcgggagtcg gctttctcgc
      481 cagcaggcct atcgcattat tcgcgatgcc ggtattgaag ctggaaccgt aacgcagact
      541 catcctcata tgttaaggca tgcttgcggt tatgaactgg cggagcgtgg tgcagatacc
      601 cgtttaattc aggattatct cgggcatcga aatattcgcc atactgtgcg ttataccgcc
      661 agtaatgctg ctcgttttgc cggattatgg gaaagaaata atctcataaa cgaaaaatta
      721 aaaagagaag aggtttaatt taacttattg atagtaaagg taaaaaa
//
LOCUS       DACBVI010000355          758 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.361, whole genome shotgun sequence.
ACCESSION   DACBVI010000355 DACBVI010000000
VERSION     DACBVI010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.361"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
BASE COUNT          284 a          125 c          147 g          202 t
ORIGIN      
        1 gaagttgatt tagatttagg cttaaaccct ttttgctttg agacagagat aaagattgaa
       61 acaagaaaga aaaatctaac tgtttcaaga ggcacagaac ttatcgaaaa gaaagataca
      121 ggtaaaagct acttcgccaa tatcgtgcat acgcaagagg tcgataaaga agagttcatt
      181 aaactctata cctctcagat aaaagcatac tttgatctca cgaaaactgc atacaaagtg
      241 tttttcatat ttttacgaat atatcaggat gcgataggca aagatcattt ctacctgagc
      301 tgtaaaaagg caatgtctct tgcggaaaag atagatcact ttgtcttgtc tgaatctatc
      361 ttttacagag ggatcaaaga actcatcgaa aaacgtatta ttgctaaaac caatgagaaa
      421 aactggtatt tcataaaccc tgctattgtc ttcaatggtg atcgtgctcg ctttgtttca
      481 gagattataa aaaagaaaga agcaatggaa gaacaacccg aaagcacagt tgtcaatggc
      541 ggcggcaata gcacacttaa agataaaaac aggtccattg aagcagttat acaagaagtt
      601 aataaccaag atgacataga tttgttaatc gcaagattgc agtctaaaaa gtctcaaggg
      661 ttagtgaaga gtcaaagtat aaatgaaatc ccttctgtag agaacacaag tcttgtgcca
      721 ccagaagaac cgttagattg ggataatatt tagtgatc
//
LOCUS       DACBVI010000356          740 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.362, whole genome shotgun sequence.
ACCESSION   DACBVI010000356 DACBVI010000000
VERSION     DACBVI010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.362"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            123..>740
                     /locus_tag="IU325_23250"
     CDS             123..>740
                     /locus_tag="IU325_23250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811885.1"
                     /translation="MIRYSGIFLSLLMLSGCATEALKNEQAETVITAGNKAVAANASF
                     FKQYYASRENFLVNFYATNPDCALRPDYTIDIGGDGNGGKLCQRSGPNTKQVQLVPFE
                     KEKLLASMEVINSMSAYVELLTKDMATTESQQVALLKQALDHANNAQTLLGVSHSKDL
                     AQSAAAVKDLVNYFNELYIIKVNSGKIGKTADENWVRLIKNMAVLR"
BASE COUNT          252 a          125 c          154 g          209 t
ORIGIN      
        1 tgaagcatga taaatagtcg cagtatttta atttaatttc atacttgctt atgaataata
       61 cccggaaata gccacattac tatttaatta tctgagataa actcaacgtc aagaggtggg
      121 ttatgattag atatagtgga atatttctat cgctgttaat gctttcaggt tgtgcaactg
      181 aagcattgaa aaacgaacaa gctgaaactg ttattactgc gggtaacaaa gcagtagcgg
      241 cgaatgcaag tttctttaaa caatactatg cttctcgtga aaacttcctt gttaatttct
      301 acgcgactaa tccggattgt gctctgcgac ctgattatac cattgatata ggcggtgatg
      361 ggaatggcgg aaaattatgc cagcgttcag ggccaaatac aaaacaagta caattagtcc
      421 ctttcgagaa agaaaaatta ctggcttcaa tggaagtcat taatagcatg agcgcttatg
      481 tggaacttct gacaaaggat atggcaacta cggaaagtca gcaagtggca ttacttaaac
      541 aggctctgga tcacgcaaac aacgcacaaa cactacttgg tgtttcacat agcaaagatt
      601 tagctcaatc tgcagcagcg gttaaagatt tggtaaatta tttcaatgag ttatatataa
      661 ttaaagtcaa ttctggaaaa ataggcaaaa cggcagatga aaactgggta agacttataa
      721 agaacatggc ggttttgcgc
//
LOCUS       DACBVI010000357          730 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.363, whole genome shotgun sequence.
ACCESSION   DACBVI010000357 DACBVI010000000
VERSION     DACBVI010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.363"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(78..257)
                     /locus_tag="IU325_23255"
     CDS             complement(78..257)
                     /locus_tag="IU325_23255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HAP5811886.1"
                     /translation="MINKLLLAYLIGLVVTSTLIFIFSEEKVTYRLFAAVITGLTWPL
                     SLIPSIISLMIRKSD"
BASE COUNT          189 a          147 c          126 g          268 t
ORIGIN      
        1 gtgttttgtt actccccgca tatattttat tctctttttc gattctattt ttcttaatcg
       61 ttcatataat tttcttgtta gtcggattta cgaatcatga gacttatgat ggatggaatg
      121 aggctaagcg gccaggtcaa ccctgtaatt actgcagcaa acaaccgata tgttactttt
      181 tcttccgaga agataaatat taaggtcgat gtcacaacca gacctatcag gtaagctaac
      241 agtaatttat tgatcacttt tacctccctt acgatttctc tttgagcggt ccttttaatc
      301 caggaccgcc tcattatgaa aactatttat ttccctcatc atgataaggt tggacatttc
      361 tttcaacata agagtcattg aggctttcga ccagtttagc ggaatgatag ggcttggcgc
      421 ttcatgcaaa gttcctgact tgtggatata gttatagctt tcgggaagtg ccatttcagg
      481 tacgctcctg ccatttgcac caatcatatt ctcgcctgtt atttgggcca atgatctatt
      541 cataaattgc actgagtcat tcaaatattc ttcttttctt gattctttat aaacaattgc
      601 tgctgatttt gcataccatg agtcaaaaaa ccattctgct tctgttgatg gaatgaagct
      661 cttttctctt ttcgcatgtg aattttgatc ggtatcagtt ttaatatcat tgaaccaaaa
      721 atttgccgac
//
LOCUS       DACBVI010000358          724 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.364, whole genome shotgun sequence.
ACCESSION   DACBVI010000358 DACBVI010000000
VERSION     DACBVI010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.364"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
BASE COUNT          240 a          129 c          155 g          200 t
ORIGIN      
        1 gagggaaaac aaccgaagac gttaggagga aattttacaa tactaatagc gaaaaaagat
       61 gggcaagcgt tggatgtaat acacaaaata atccactgtt cagacgattc aaatatttta
      121 ccaaggtgtg tttcataact attttgtgta caatatgact ctacatatcg acgaaaaaac
      181 cacttgattt gacaggaaca cattcaaaaa aatttctttt tcatttcagg agcgattttc
      241 atctgttact tgaagtatct gtgagattaa gtgactcccc ctaacgtcgt actaaatagt
      301 aattactggc cataccggga gcagtattcg gcgcttccca catgcagcag aataaccgca
      361 tcagcgaaga tgtattgtat ttttactatc acaatgtgta cggacggttt atggaaaaga
      421 atgaacatta gatacgcgat gcggcggcag attacacggt tatcactacg atagccaata
      481 ctggctggtg cattaccgcc gtgaacaata ggacgccgta ctgctggaaa tttcttacct
      541 ttatgactcc ccgcaccgcc gtatcgggaa acgggaagca gtcaagatga ggctgatata
      601 atgtgtggaa tgcctgagta tttttagaat agagggtaat atgtttgata gaatatatct
      661 tggtgatcga ccaataaaaa aattgaattt gatttatgga gcaaacaaat tagaattcag
      721 gtga
//
LOCUS       DACBVI010000359          723 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.365, whole genome shotgun sequence.
ACCESSION   DACBVI010000359 DACBVI010000000
VERSION     DACBVI010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.365"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(13..381)
                     /locus_tag="IU325_23260"
     CDS             complement(13..381)
                     /locus_tag="IU325_23260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HAP5811887.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPETKK"
     gene            complement(544..>723)
                     /locus_tag="IU325_23265"
     CDS             complement(544..>723)
                     /locus_tag="IU325_23265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 family protein"
                     /protein_id="HAP5811888.1"
                     /translation="QHPASRLFPFCTGKYRWHGSAEAYTGREVQDIPGVLAVFAERRK
                     DSFGPYVRLMSVTLN"
BASE COUNT          167 a          192 c          207 g          157 t
ORIGIN      
        1 tattctggtg gtttactttt tcgtttcagg tgtcggataa acagccagat aaacgtaacc
       61 acaggagccg agggtgtcgg cttcacaggt cagccccttc gcatacagtg tcacggtatg
      121 ctgatggcgg ggattcagtt caccactggt gagcattaat ttcagttgtt tcatcagcag
      181 cggaaaggcc tggtccagag ggtacgcatc cgcatctctg aaccggcctc tgataccggc
      241 acggtcggca aggtaatgca accggttacc ctcctgcacc agacgggcgc cgaaacaggg
      301 cgtcactgtg cagggtagtc cccaccaggg atggtcgtca ttgtcatcgg gatgcgttgt
      361 cccggaaagt gtgtctgaca cgataaaatc ctcacagaaa atcggtgaat gatagtttaa
      421 cgatgacggg tcagccggcg cgcagaccgg cttcggtaaa ggtgtattcg ttgatgagca
      481 ggacctcatc cacagcggcg tctgagctca gccagtcgta ctcgttttcc gggtgacgga
      541 acatcagttc agggtgacgc tcatcagccg gacatacgga ccaaaactgt ccttacggcg
      601 ttctgcaaac acggccagca caccgggaat atcctgcact tcacgaccgg tatacgcttc
      661 agcactgccg tgccagcggt atttgccggt acagaacgga aaaagacggg atgccggatg
      721 ctg
//
LOCUS       DACBVI010000360          721 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.366, whole genome shotgun sequence.
ACCESSION   DACBVI010000360 DACBVI010000000
VERSION     DACBVI010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.366"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            1..420
                     /locus_tag="IU325_23270"
                     /pseudo
     CDS             1..420
                     /locus_tag="IU325_23270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          200 a          166 c          202 g          153 t
ORIGIN      
        1 actgaccggg tgagcgagga aagccgccgc tggctggcat cgtgcggcct gacggtggag
       61 cagatgcaaa accagatgga cccggtgtac acgccggcgc gaaaaatcca cctgtaccac
      121 tgcgaccatc gcggcctgcc gctggcgctt gtcagcacgg aaggggcaac agaatggtgc
      181 gcagaatacg atgaatgggg caacctgctg aatgaagaga acccgcatca gctgcagcag
      241 ctcatccgcc tgccgggtca gcagtatgat gaggagtccg gcctgtatta caaccgccac
      301 cgctattatg acccgctgca ggggcggtat atcactcagg atccgattgg gcttaagggg
      361 ggatggaatt tttatcagta tccgttaaat ccgatctcag agatagaccc acaagggtta
      421 aatccgttaa ttcttagtgt tgtagcactg atgagttgcg gtttgttttc atcaggtggt
      481 gttatccaaa gtgggcaaca acgcagagat caatatggcc gtaaagttaa tccagaagcc
      541 gacaaattaa cagatgaaca taagtcaggt gtaaatcctg gtggtaactg tagtccccag
      601 aacctgagtg acttacaaaa tgaaaaaaac agattatgta accagtctag agcttgcgct
      661 cctcagatgc tgagaaaaga aatattgaga cggtacgaga taaatttggc gtgcgcttcg
      721 g
//
LOCUS       DACBVI010000361          699 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.367, whole genome shotgun sequence.
ACCESSION   DACBVI010000361 DACBVI010000000
VERSION     DACBVI010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.367"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..180)
                     /locus_tag="IU325_23275"
     CDS             complement(<1..180)
                     /locus_tag="IU325_23275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP5811889.1"
                     /translation="MDLNTAANALRELGHPTRLSIYRELVRAGHEGLPVGELQKHLEI
                     PASTLSHHLSALISAG"
BASE COUNT          176 a          178 c          175 g          170 t
ORIGIN      
        1 tccagcggat atcagcgctg aaagatgatg gctgagcgtg gaggcaggaa tctcaaggtg
       61 cttctgcagt tcgccaaccg gcaggccttc atgacccgcc ctgaccagct cccggtatat
      121 gctgagacgt gtcgggtggc ccagttctcg aagtgcgttt gcagcagtgt ttaaatccat
      181 aatacctcct ttatctatat ttccagaata atagaaatat agcctgcgtc aatcgtttct
      241 gccgtgaggg taccgctttc ccaatcatgc tctgtccgcc aggttatggg gggcagagcc
      301 agagcactac cgaaatcctt gcgaataccc gtgcagggaa taatttccgc cccctttatg
      361 gccggaaaaa aaactgataa acgtcgcctt ttcagaaggt tgctttctta acgtggtttt
      421 tcgcatggtt ggacctcaga cccccttatc gttcagcgag aaaaagaagc gttggtcggc
      481 ggaggtgaaa gcggggggtc catataactc atcttgttct gcctcggaca agatttggac
      541 acgttcttcg aatgccatgg atttagccca aaaagactaa atcttactca aacagagccc
      601 aaatagggat ctcgaaaaaa gtaatacccc tctggaggcc acagacggcg gggcctccag
      661 agcactttgt cgtttttgga cggaaaatcc ctagaaccc
//
LOCUS       DACBVI010000362          695 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.368, whole genome shotgun sequence.
ACCESSION   DACBVI010000362 DACBVI010000000
VERSION     DACBVI010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.368"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..>695
                     /locus_tag="IU325_23280"
     CDS             <1..>695
                     /locus_tag="IU325_23280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HAP5811890.1"
                     /translation="LDRREVLHTQGEGGLKRVVKKEHADGSVTQSQFDAVGRLKAQTD
                     AAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQLTSATGPDGLELRREYDESGR
                     LIQETAHNGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLTVTDCSGYVTRY
                     DHDRFGQMTAVHREEGLSQYRAYDSRGQLIAVKDTQGHETRYEYNAAGDLTAVIAPDG
                     SRNGTQYDAWGKPV"
BASE COUNT          171 a          194 c          219 g          111 t
ORIGIN      
        1 ctggaccgcc gtgaagtgct gcacacgcag ggcgaaggcg ggctgaagcg ggtggtgaaa
       61 aaggaacacg cggacggcag cgtcacgcag agtcagtttg acgcggtggg caggcttaag
      121 gcacagacgg atgccgcagg ccggacgact gagtacagcc cggatgtggt gacgggactc
      181 atcacgcgca tcaccacacc ggatggcagg gcatcggcat tttactataa ccaccacagc
      241 cagttaacgt cagccaccgg gcctgacggg ctggaattgc gccgggaata tgatgaatcc
      301 ggccgtctga ttcaggaaac cgcacacaac ggcgatatca cccgctaccg ttatgataat
      361 ccacacagtg acttaccctg cgcaacggag gatgccaccg gcagccggaa aaccatgaca
      421 tggagccgtt acggtcagtt gctgaccgtc actgactgtt ccggttatgt aacccgttat
      481 gaccatgacc gttttggtca gatgacggcg gtgcaccgcg aggaagggct gagtcagtac
      541 cgcgcatacg acagccgtgg acagttaatt gcggtgaaag acacgcaggg ccatgaaacg
      601 cggtatgaat acaacgccgc cggtgacctg accgccgtca ttgccccgga cggcagcaga
      661 aacgggacac agtacgatgc gtggggaaag cccgt
//
LOCUS       DACBVI010000363          695 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.369, whole genome shotgun sequence.
ACCESSION   DACBVI010000363 DACBVI010000000
VERSION     DACBVI010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.369"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..>695
                     /locus_tag="IU325_23285"
     CDS             <1..>695
                     /locus_tag="IU325_23285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811891.1"
                     /translation="LDRREVLHTQGEGGLKRVVKKEHADGSVTQSQFDAVGRLKAQTD
                     AAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQLTSATGTDGLEIRREYDESGR
                     LIQETAPDGDITRYRYDNPHSDFPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRY
                     DHDRFGQVTTVHREEGLSQYRAYDSRGQLIAVKDMQGHETRYEYNAAGDLTAVIAPDG
                     SRNGTQYDTWGKA"
BASE COUNT          172 a          195 c          217 g          111 t
ORIGIN      
        1 ctggaccgcc gtgaagtgct gcacacgcag ggcgaaggcg ggctgaagcg ggtggtgaaa
       61 aaggaacacg cggacggcag cgtcacgcag agtcagtttg atgcggtggg caggcttaag
      121 gcacagacgg atgccgcagg ccggacaaca gaatacagcc cggatgtggt gacgggcctc
      181 atcacgcgca tcaccacgcc ggatggcagg gcatcggcgt tttactataa ccaccacagc
      241 cagttaacgt cagccaccgg gactgacggg ctggaaatac gccgggaata tgatgaatcc
      301 ggccgtctga ttcaggaaac cgcccctgac ggcgatatca cccgctaccg ttatgataat
      361 ccacacagtg acttcccctg cgcaacggaa gatgccaccg gcagccggaa aaccatgacg
      421 tggagccgtt acggtcagtt gctgagcttt accgactgtt ccggttatgt aacccgctat
      481 gaccatgacc gttttggtca ggtgacgacg gtgcaccgcg aggaaggcct gagccagtac
      541 cgcgcatacg acagccgtgg acagttaatt gcggtgaaag acatgcaggg ccatgaaacg
      601 cggtatgaat acaacgccgc cggtgacctg accgccgtca ttgccccgga cggcagcaga
      661 aacgggacac agtacgatac gtggggaaag gccat
//
LOCUS       DACBVI010000364          694 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.370, whole genome shotgun sequence.
ACCESSION   DACBVI010000364 DACBVI010000000
VERSION     DACBVI010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.370"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            255..635
                     /locus_tag="IU325_23290"
     CDS             255..635
                     /locus_tag="IU325_23290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811892.1"
                     /translation="MTNVDGKILAIMGLITGLSGQAKVSHILFEQAKIHTTDIASLYY
                     YRALVNFHNERIEEARICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKSNMKLYYKET
                     SSESHRVIIDNILKLKQLTRICMR"
BASE COUNT          249 a          110 c          131 g          204 t
ORIGIN      
        1 aaggttataa gaatcattat ttatcagatg aactgttggc tggtaagaaa gaactttacg
       61 agtttactcc tgaaagcatt tacagggcta tggctatatt tgatggatta caaaataaaa
      121 gtgatataca gacgctaaaa atagaatgtt attgtctgct agcggaatgc catatgtctt
      181 tggcacttca tggaaagtat gaacttgaac ttgctgctca aaaagcatta gagcttttac
      241 attatgtatc agacataacc aatgtcgatg gaaaaatttt agctattatg ggactgataa
      301 ctggtctgtc tggacaagca aaagtatctc atatcttatt tgaacaggct aagatacaca
      361 caactgatat agcctctctc tactactata gagcacttgt caattttcat aatgaaagaa
      421 ttgaagaggc aaggatttgt atagacaaat cactacaact cgaacccaga agacgaaaag
      481 cagttgtgat aaaagagtgt gtagatatgt atgtgcctaa cccgctcaaa agcaacatga
      541 aactctacta taaagaaact tcgagtgaaa gccatcgagt tataattgac aacattttga
      601 aactaaagca actgacgaga atttgtatgc gataaatctt tattgtcgca attttattgt
      661 ttagatgtat gcaactggtt atgtcaacaa caga
//
LOCUS       DACBVI010000365          691 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.371, whole genome shotgun sequence.
ACCESSION   DACBVI010000365 DACBVI010000000
VERSION     DACBVI010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.371"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
BASE COUNT          262 a          128 c          124 g          177 t
ORIGIN      
        1 aataatatta gcaatataaa caatacagct cataataaga atctcgataa aacaacattc
       61 gatgaacaac ctgcttatga aacaagagta tcacaagcta aaaaaatact gcaaagtttt
      121 gcgccaaatg atattaaagt tgatacaaat actaaattta ttagtctgat agagtctgtc
      181 cgtgctctct ctcctgaatt acaatgtaag gtactagatg gtcttaatta tgctaagaat
      241 acactacata atataaatca aaattctgcc atagcaacaa aatcatcctg tcagtctacc
      301 gagtcaatag agaaaaccat aacatttaat attctcgata tgttagataa tttgacgatg
      361 agacaacaga acattatgga ggcctttcta agagtcttta catcaatgta caatgcctct
      421 gccaaggctg atgctagcat gagcatcgtt tttgaacagc ttgtccatct tcagagtagc
      481 gaaacaaaaa atgcttcttg ggcaaatttt gcaggtgcat tagcaggctc agctattaat
      541 gctacgggag ctgtatttat ggcaggtgca gaacttgcga agcccgcggg aaagatagat
      601 gatatcgcag aaaaccagga acaaaataca gacaaaccag atagccaaac tcggataaaa
      661 tcaaaagaag atatggcaaa tgcgaaagcc a
//
LOCUS       DACBVI010000366          690 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.372, whole genome shotgun sequence.
ACCESSION   DACBVI010000366 DACBVI010000000
VERSION     DACBVI010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.372"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            115..435
                     /locus_tag="IU325_23295"
                     /pseudo
     CDS             115..435
                     /locus_tag="IU325_23295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          200 a          147 c          165 g          178 t
ORIGIN      
        1 tcgtaaaagg tataaatggt ttaataaaaa acttgtctac tcgttggagt agatgaaaaa
       61 taagatgacg ttggcatata gatcactgcg aataatccac ctctaccact gcgaataatc
      121 cacctctacc actgcgacta tcgcggcctg ccgctgacgc ttatcagccc tgacggggca
      181 acagaatggt gcgcagaata tgatgaatgg ggcaacctgc tgactgaaga gaacccgcag
      241 cacctgcagc agcttatccg tctgccgggg cagcagtatg atgaggagtc cggcctgtat
      301 tacaaccgcc accgctatta tgacccgctg caggggcggt atatcactca ggaaccgatt
      361 gggctggaag gcgggtggaa tcagtatgta tatgcttcca tacatccaac ctattctatt
      421 gatccgctgg gcttaattga taaacctgct ccagtattca atcgagaact aaattcagat
      481 gcgtattatc ttgccgtaaa taattgctat tcatatgctc taaataggta tggaaatcct
      541 gggagtagga tttttggcgg cggaggatta caacctggag aattatctgg aaaagagttt
      601 tctaaactca cttgtagtag tatttttgaa gcgtcaaaaa atgatggtgc caaggacagg
      661 gaaagatcat cagaagtact ctttttgtac
//
LOCUS       DACBVI010000367          670 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.373, whole genome shotgun sequence.
ACCESSION   DACBVI010000367 DACBVI010000000
VERSION     DACBVI010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.373"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            410..595
                     /locus_tag="IU325_23300"
                     /pseudo
     CDS             410..595
                     /locus_tag="IU325_23300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005183775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
BASE COUNT          215 a          123 c          141 g          191 t
ORIGIN      
        1 aaaaaataat ttcttgcgaa attagcagcc cattttgaat aagtgtgttc cggagtaatc
       61 cagagcgcat gctgctttac gtctttttca agccatattg gttaagcaaa aagaggtaga
      121 aataatttcc tgagaaatta actcaacatt ctgaaacggc cagttttctg aaattaccag
      181 aagaccgaat acattcatgc ctgaagaatg gattagaaga aacgagacgg aaatagatct
      241 atttatccct gtggaaataa tttctgctga aattgtttgc ggctattaaa aaggtcaaac
      301 cgtctgacta aattttatcc agttacaaac aagtattacc tccagtgtat tggtagctgt
      361 taagccaggg gcggtagcat acctgaagag aataggatca catcaccaaa tggcaagaag
      421 tggatttgaa gtccagaaag tcaccgtaga ggcattattt ctacgagaaa tacgaacacg
      481 ctttggtaag ttccgtctgg gatatctgtg ggcgattctc gaaccctctg cgcatttgct
      541 gatactattg ggcatttttg gttacattat gcaccgcacg atgccagata taacatgatt
      601 ctccgcgcta aacttacatt caatatggag atgtttagtg aagattaaac gaaaatttgc
      661 cagtggactt
//
LOCUS       DACBVI010000368          660 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.374, whole genome shotgun sequence.
ACCESSION   DACBVI010000368 DACBVI010000000
VERSION     DACBVI010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.374"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..>660
                     /locus_tag="IU325_23305"
     CDS             <1..>660
                     /locus_tag="IU325_23305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAP5811893.1"
                     /translation="PISEDSKLLNMLSEFKLLREQCFRWGNYTLLFENYGAYDKTGSI
                     TIEKSQGEGTLPIRHKLEFISTNIAELLDKLTKITDTRLCKGFSDWASSVKEGASNDF
                     KENVDRALLRMFKCVELHNNELDLSYLFLGSVPPLPEWIEMLSLIHNELDSIHVPESC
                     KELEVDFNNLTEFPQVPDGITLISVNNNLISHIDSFPPKAKIISICHNKLSEIPTIPD
                     A"
BASE COUNT          218 a          119 c          131 g          192 t
ORIGIN      
        1 caccaattag tgaagatagc aaattactga atatgctgtc tgaatttaaa ttattgagag
       61 agcaatgttt caggtggggt aattatactc tattgtttga aaattatggg gcttatgata
      121 agacggggtc tatcacgata gaaaaaagtc agggggaggg gactttaccc attcgacata
      181 aattagagtt tatatcgacc aatattgcag agttgctgga caagttaacc aaaattacag
      241 ataccagact ttgcaaaggt ttcagtgact gggctagttc agtcaaagaa ggcgcatcca
      301 atgacttcaa agaaaatgtg gatagggcat tgctgagaat gtttaaatgt gttgagctcc
      361 acaataatga acttgactta tcatacctat ttttgggttc tgtaccgcct cttcccgagt
      421 ggattgaaat gcttagcctt attcataatg aacttgattc aatacatgtg cccgaatcat
      481 gcaaagaatt agaagtcgat ttcaataacc ttacagaatt tccacaagta cctgatggaa
      541 ttaccctgat ctccgtaaat aataacctga tatcgcatat tgactcattt ccgccaaagg
      601 ctaagataat ttctatttgt cacaataagc tatcggaaat accaacaata ccagacgccg
//
LOCUS       DACBVI010000369          645 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.375, whole genome shotgun sequence.
ACCESSION   DACBVI010000369 DACBVI010000000
VERSION     DACBVI010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.375"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            352..>645
                     /locus_tag="IU325_23310"
     CDS             352..>645
                     /locus_tag="IU325_23310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP5811894.1"
                     /translation="MIRIAFLITNKFLEIDSTTLAGYDFSGMNLHRATLNGYCLDGAK
                     FEKTHLRNVMIQHTSTRNAVFHNAALMNAILNNSDFTGSDCSNSRSIGENFKAI"
BASE COUNT          219 a          110 c          139 g          177 t
ORIGIN      
        1 cacaaatgtt ggtgacattg ataatattgg tgaatgtcaa attctcatca ggtataaaga
       61 ccgctggatt gagaataaac tcaaagaaaa aataattgaa taaccatgtc agttatagac
      121 attttagatg gaacgaacca gagaaggtca tgcctccgag ctgggttatg tggacacgga
      181 gctttagcgt agtgaactca acgaagcaag ccggaaacct gccagccctt atcagcacgt
      241 aagggacaac agcgtagtac gcagaataat cctacccacg tagtatggac acaggcctaa
      301 gcgaggttct ggttttcaaa tttttcagga gttagatgat tggaggaaca gatgataaga
      361 atcgcatttt taataaccaa taaatttctt gagatagata gtaccactct tgctggatat
      421 gattttagcg ggatgaatct acatcgtgcc accctcaatg gttattgtct tgatggggcg
      481 aaatttgaaa aaactcattt aagaaatgta atgattcaac atacatcaac tagaaatgct
      541 gtctttcaca atgcggctct gatgaatgct attctgaata actcagattt cactggaagt
      601 gattgtagta attcaaggtc aataggtgaa aattttaaag caata
//
LOCUS       DACBVI010000370          639 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.376, whole genome shotgun sequence.
ACCESSION   DACBVI010000370 DACBVI010000000
VERSION     DACBVI010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.376"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            5..319
                     /locus_tag="IU325_23315"
     CDS             5..319
                     /locus_tag="IU325_23315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811895.1"
                     /translation="MYNQKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHL
                     TSKKVNKFEIMDQVDDDRLAELIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHC
                     AR"
     gene            complement(352..432)
                     /locus_tag="IU325_23320"
     CDS             complement(352..432)
                     /locus_tag="IU325_23320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811896.1"
                     /translation="MKDVDQNFDALYWHILQAYLILLFYD"
BASE COUNT          252 a           96 c          106 g          185 t
ORIGIN      
        1 ctttatgtat aaccaaaaaa ataaaacagg aactgttgcg ttgagcggaa cctggcaaca
       61 aggtaacaga gaaagtaagt caatacggcg aaatattgaa tacacatgga ttgaaaacta
      121 tgacacagcc catttaacat caaagaaagt taataaattt gagatcatgg atcaagtcga
      181 tgatgataga cttgcagaac ttattcctga tttttatgtc tttccagaaa aaagtgtaag
      241 ctataatata ctaaagcaag gtaagcatgc ttttattttg agtattggta acagagcaat
      301 aatgcattgt gcaaggtaaa cttaaataga taataaaaag atggaacctc gttaatcata
      361 aaacaataaa attaaatatg cttgtagtat atgccagtat aaagcatcaa agttttgatc
      421 aacatctttc attttagata tcaccttgca atgcaaaata tcatgtatca acaacatctg
      481 gtgcaaatcc atgagtcgaa ctcaacattc atcttagctc tattattatt ccttcgtaca
      541 gtcgatgtaa acaatacaga aagaggatta ttaagaacca gtttacttag agaatcaata
      601 gaggaaagga ctaacgtttc tttaaaagaa ttgatttca
//
LOCUS       DACBVI010000371          634 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.377, whole genome shotgun sequence.
ACCESSION   DACBVI010000371 DACBVI010000000
VERSION     DACBVI010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.377"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            230..529
                     /locus_tag="IU325_23325"
     CDS             230..529
                     /locus_tag="IU325_23325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001491987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP5811897.1"
                     /translation="MGALKLLDDKHGELKSMRTAPNYLRRGVASLILRHILQVAHDRC
                     LHRLSLETGTQAGFTACHQLYLKHGFVDCDPFADYQFDQHNRFLSLTLCENNELP"
BASE COUNT          172 a          143 c          152 g          167 t
ORIGIN      
        1 tggtcgtaac aacatccgac gtggtgatgt gtcaaatgag acactctgat gatcagaggg
       61 tttatcgtgt atacggatcg tggatggcag aaaactttca ggatcaggta tccattttaa
      121 accagataat gagtaagttt gccccatcca tgccccacgc ggtgagatcg gatgtcatta
      181 aaaaagtcag gtcagcaggt cagtatggga aggcgaacaa ctcgcaggaa tgggtgcgct
      241 gaagttgctg gatgataaac atggcgagct gaaatcaatg cgtaccgcgc caaattattt
      301 acgtcggggt gtcgccagtc tgattttacg ccacattttg caggtcgccc atgacagatg
      361 ccttcatcgc ctgagtttag aaacgggtac acaggctgga tttacggcct gccatcaact
      421 ttatttgaag catggtttcg ttgattgcga tccgtttgct gattatcaat ttgatcaaca
      481 caatcgattt ttatcattga cgctatgcga aaataatgaa ttgccataag ccagacgcag
      541 cacattcttg cattcgacgt gctgcgcctt tatttatcac cagcaggaaa cgccttgtcc
      601 atagacgcca cttctacatg cgccacaaga aacc
//
LOCUS       DACBVI010000372          632 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.378, whole genome shotgun sequence.
ACCESSION   DACBVI010000372 DACBVI010000000
VERSION     DACBVI010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.378"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            74..613
                     /locus_tag="IU325_23330"
     CDS             74..613
                     /locus_tag="IU325_23330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP5811898.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPDVSATKLQNNGQVSGVQTDVPIELKDCDITVTKNATFTFNGTADT
                     TQITAFANQASTDAATNVALQMYMNDGTTTITPDTETGNILLQNGDQTLTFKVDYIAT
                     GKATSGNVNAVTNFHINYY"
BASE COUNT          199 a          114 c          139 g          180 t
ORIGIN      
        1 aattattgtt attattaagt ctaatgtcat ttaaaggcat tatcatattt acgaagagat
       61 caggatgata acgatgaaaa aaagtgtatt gacggcgttt ataactgtgg tatgtgcaac
      121 gtccagtgtt atggcagctg atgataatgc tatcacggat ggctcagtaa catttaatgg
      181 taaagtcatt gccccagcgt gtacgctggt agctgcgacg aaagattccg tggtgacttt
      241 gccagatgtt agtgctacga agttgcaaaa taatggccag gtttctgggg tgcaaactga
      301 tgtgccaatt gaattaaaag attgtgatat taccgtaaca aaaaatgcaa cgttcacctt
      361 taatggcact gcggatacta cgcagattac agcgtttgct aatcaggcct ctactgatgc
      421 tgctacaaac gtggccctgc aaatgtacat gaatgatggc acaacgacca tcacgccaga
      481 cacagaaacc gggaacatct tgttgcagaa tggagatcag acgttgacct ttaaagttga
      541 ttatatcgct acgggtaaag ccacttcagg taatgttaat gcggtaacaa acttccatat
      601 taactattat taatagaaca cattaattgt tt
//
LOCUS       DACBVI010000373          631 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.379, whole genome shotgun sequence.
ACCESSION   DACBVI010000373 DACBVI010000000
VERSION     DACBVI010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.379"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..>631
                     /locus_tag="IU325_23335"
     CDS             <1..>631
                     /locus_tag="IU325_23335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HAP5811899.1"
                     /translation="ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEF
                     AAVIPDNDSTQAACAKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPYLLKLLRGETK
                     YGSLKYLDQDALNIAFNMNNIYLAKDFDTIYTLKNELYDRSHRKYQQTITDKTVLIHY
                     TGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHL"
BASE COUNT          204 a          119 c          123 g          185 t
ORIGIN      
        1 acgcgacata cttcagggta ttgtcttttg aatatctgag tgaaagtatt tccacactgc
       61 tgtatctgga tgccgatgtt gtttgtaaag gaagcctgaa acctctcaca gaaattatat
      121 ttaaagatga gtttgctgcg gtcattcctg acaatgatag tactcaggcg gcatgtgcaa
      181 aacgcctcaa cattcccgaa atgaatggac gttatttcaa tgcaggcgtt atctatgtca
      241 atcttaaaaa atggcatgaa gcaaatttga caccgtattt actcaaactt ttacgagggg
      301 aaactaaata tggctctctt aaatatttag atcaggatgc gttgaatatc gcatttaata
      361 tgaataatat ctaccttgcg aaggattttg atactattta taccctgaaa aacgaacttt
      421 atgatcgtag tcatcgaaag tatcagcaaa ccattaccga taaaacagtg ttgattcact
      481 atacagggat aactaaacca tggcatagct gggctggata tccgtctgca tcatacttta
      541 atatcgcgcg tgaacaatct ccctggaaga aatatcctct taaagaggcg cggactgttg
      601 cagaaatgca gaaacaatat aagcatctgt t
//
LOCUS       DACBVI010000374          624 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.380, whole genome shotgun sequence.
ACCESSION   DACBVI010000374 DACBVI010000000
VERSION     DACBVI010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.380"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            19..534
                     /locus_tag="IU325_23340"
     CDS             19..534
                     /locus_tag="IU325_23340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HAP5811900.1"
                     /translation="MSNPVLRSITNEMILLQYNLSVEHFSLNSSLIYYINNWKLFPLI
                     CLLSGCHFYRERFAERGFFYKIPEVLRNYLSAIPVEINEKARYKPGIVNYQNIITCGF
                     STLLPYVRQQPLAMQQRFNLLFPDFVDHIQLPLPLASTLLERITFYAKKNRDELDKLS
                     CKWCCDQKNNF"
BASE COUNT          211 a          103 c          112 g          198 t
ORIGIN      
        1 agtttgaata atgcacgtat tagcaatccg gtacttagat ctattaccaa tgaaatgata
       61 ctcctacagt acaatctttc agttgagcat ttcagtctga atagttcact tatttactac
      121 ataaacaatt ggaaattatt tccattgatc tgtttactca gtggttgtca cttttatcga
      181 gaaaggtttg cagaacgcgg atttttttac aagattcctg aagtgctaag gaattattta
      241 tcagcgatac cggtggaaat aaatgaaaag gcacgatata aacctggtat cgttaactat
      301 cagaatatca tcacatgtgg tttttcaacg ttgttaccct atgtaaggca acaaccgttg
      361 gcaatgcagc aacgatttaa tttattattt cctgattttg ttgaccacat acaattgccg
      421 ttaccacttg cgtctactct tttggagaga attacatttt atgcgaaaaa aaatagagat
      481 gagcttgata aactctcctg caaatggtgt tgtgatcaaa agaataattt ctgatggatt
      541 gaaagaaata gtttctctaa aagaacagat ccttcttgaa accaccgcaa aaattcagag
      601 tattgaggaa aagagagaag aaaa
//
LOCUS       DACBVI010000375          623 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.381, whole genome shotgun sequence.
ACCESSION   DACBVI010000375 DACBVI010000000
VERSION     DACBVI010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.381"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..>623
                     /locus_tag="IU325_23345"
     rRNA            <1..>623
                     /locus_tag="IU325_23345"
                     /product="23S ribosomal RNA"
BASE COUNT          171 a          135 c          196 g          121 t
ORIGIN      
        1 aatgggtcag cgacttatat tctgtagcaa ggttaaccga ataggggagc cgaagggaaa
       61 ccgagtctta actgggcgtt aagttgcagg gtatagaccc gaaacccggt gatctagcca
      121 tgggcaggtt gaaggttggg taacactaac tggaggaccg aaccgactaa tgttgaaaaa
      181 ttagcggatg acttgtggct gggggtgaaa ggccaatcaa accgggagat agctggttct
      241 ccccgaaagc tatttaggta gcgcctcgtg aattcatctc cgggggtaga gcactgtttc
      301 ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact gcgaataccg gagaatgtta
      361 tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa gagggaaaca acccagaccg
      421 ccagctaagg tcccaaagtc atggttaagt gggaaacgat gtgggaaggc ccagacagcc
      481 aggatgttgg cttagaagca gccatcattt aaagaaagcg taatagctca ctggtcgagt
      541 cggcctgcgc ggaagatgta acggggctaa accatgcacc gaagctgcgg cagcgacact
      601 atgtgttgtt gggtagggga gcg
//
LOCUS       DACBVI010000376          622 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.382, whole genome shotgun sequence.
ACCESSION   DACBVI010000376 DACBVI010000000
VERSION     DACBVI010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.382"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..>622
                     /locus_tag="IU325_23350"
     CDS             <1..>622
                     /locus_tag="IU325_23350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811901.1"
                     /translation="IVNEVVKTISLPIEKDDNGYYDFSGLYLAHSNISGKDPNKDPDI
                     DFGIDLGNCNCSNVNFEHTYFDSVKFTNTNCTNANFNSCRFIKCDLTNMNCTGAILDN
                     AVIYGKEKEPEMQYPEADQIIQRITYQKSHGNETKGMILTNCSCVKTTFNWADLSESD
                     CQNVDFSEANLSNTILPDIVRMKGTKLYRTDLFNPILKAEAESTEEK"
BASE COUNT          232 a          108 c          114 g          168 t
ORIGIN      
        1 tattgttaac gaggtagtaa agactatttc actcccaata gaaaaggatg acaatggata
       61 ttacgatttc agcggtctat atctagcaca cagcaatatt tccggtaaag atcccaataa
      121 agatcccgat atagatttcg gtatagatct gggaaactgt aactgctcaa atgtcaactt
      181 cgaacatact tattttgata gcgtcaagtt tacaaatacg aattgtacaa atgcgaattt
      241 taacagttgt agatttatca aatgcgatct aacaaatatg aattgcactg gcgcaattct
      301 tgataatgcg gtgatatatg gtaaagaaaa agaacctgag atgcaatatc cagaagcaga
      361 tcaaataata caaaggataa cttatcagaa atcacatggg aatgaaacga aagggatgat
      421 tctaactaac tgctcctgtg ttaaaaccac tttcaattgg gcagatttat cagaaagtga
      481 ttgccaaaat gtggacttta gcgaggctaa tctttcaaac actattcttc ctgacatcgt
      541 caggatgaaa ggcacgaaac tttaccgtac cgaccttttt aatccgatat taaaagcaga
      601 agcagaatca acagaagaaa aa
//
LOCUS       DACBVI010000377          617 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.383, whole genome shotgun sequence.
ACCESSION   DACBVI010000377 DACBVI010000000
VERSION     DACBVI010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.383"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
BASE COUNT          204 a          117 c          114 g          182 t
ORIGIN      
        1 cagggaactg gtaacccacc cattcggcat aaattagagt ttatatcgac taatattgca
       61 gagtttctag acaacttaac gaaaattaca gattccagac tttgcaaagg tttcagcgac
      121 tgggctaatt cagtaaaaga aggcgcatcg aatgacttca aaaaaaatgt ggatatagca
      181 ttggtgagat tgtttaaatg tgttgaactc cacaatagta aacttgactt aacagatctt
      241 cacttggggt ctttgccacc tcttccagac tggattgagg tgctttctct tcgtcataat
      301 ggccttgcca caatacaaat tcccaaattc tgcaaagaat tagaactcga ttttaataat
      361 tatatggtgt ttccaaaagt atccgatgga attacccagg tctccgtaga taataatctg
      421 atatcgcgtg ttgactcatc tccgtcaaag gctatgaaaa tttttattta tcgcaataaa
      481 atatggtaaa taccaccaat acctgagacg actaagctgt ttaattgtgg cgataataag
      541 acaaaagaaa ttcaatactt cccaaatcat ctggagaaag cacgtatcga gtataatatt
      601 gaggttgtcc ctgcgat
//
LOCUS       DACBVI010000378          614 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.384, whole genome shotgun sequence.
ACCESSION   DACBVI010000378 DACBVI010000000
VERSION     DACBVI010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.384"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..481
                     /locus_tag="IU325_23355"
     CDS             <1..481
                     /locus_tag="IU325_23355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP5811902.1"
                     /translation="TLEIFNNSDEWASQLKHALSQGENLALLHGLTPDILDRIYAYAF
                     DYHEKGNITDAEIYYKFLCIYAFENHEYLKGFASVCQSKKKYQQAYDLYKLSYNYSPY
                     DDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDESVKSKAQAYIELLTDNSEDNG"
BASE COUNT          227 a          102 c           99 g          186 t
ORIGIN      
        1 tacacttgaa atattcaata acagtgatga atgggcaagt caactaaaac acgcattatc
       61 acaaggagaa aatctggcat tactacatgg attgactcct gatatccttg atagaatata
      121 tgcatatgca ttcgactacc atgaaaaagg taatataacg gacgcagaaa tttattataa
      181 atttctgtgc atttatgcgt tcgaaaatca tgaatatcta aaaggttttg catcagtatg
      241 ccagtcaaaa aagaaatatc aacaagcata tgacctttac aaactaagtt acaattactc
      301 cccgtatgat gactattcag ttatttatcg tatgggtcaa tgtcaaattg gggctaaaaa
      361 tatcgataac gcaatgcaat gtttctatca tattattaac aattgtgagg atgaaagtgt
      421 taagagcaaa gcgcaggcat atattgaact cttaaccgat aattcagaag ataatggcta
      481 agcattacaa gcatttggta aaaattgctg gttatagtgc agttaaccac tatgaccagc
      541 ttctcttatg ttttcttaaa tcaacatctt aaaacagtta agaacacact ataaattact
      601 atttttcaac gcgt
//
LOCUS       DACBVI010000379          608 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.385, whole genome shotgun sequence.
ACCESSION   DACBVI010000379 DACBVI010000000
VERSION     DACBVI010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.385"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            28..>608
                     /locus_tag="IU325_23360"
     CDS             28..>608
                     /locus_tag="IU325_23360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898147.1"
                     /note="Required for surface presentation of invasion
                     plasmid antigens; required for invasion and for secretion
                     of the three ipa proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HAP5811903.1"
                     /translation="MLFKTLDGDIISLLSDWGEMLFLLILYCLGSMIIVLIFDFIAEH
                     FLFMKDMKMDKQEVKREYKEQEGNPEIKSKRRERHQEILSEQLKSDVSNSRLMIANPT
                     HIAIGIYFKPHLSPIPLISVRETNEVALAVRKYAKEIGIPIITDKKLARKIYATHRRY
                     DYVSFENIDEILRLLLWLEDVENAGQPVPDELLP"
BASE COUNT          208 a          102 c          116 g          182 t
ORIGIN      
        1 aaattatttt ggagtaacca taaatcactg ctttttaaaa ctcttgatgg agatatcatt
       61 tctttattat cagattgggg agagatgctg ttccttctca tactatattg tctcggcagt
      121 atgataattg ttctaatatt tgattttatc gctgagcatt ttttatttat gaaagatatg
      181 aaaatggata aacaagaagt taaaagagaa tacaaggaac aagaaggaaa tccagaaatt
      241 aagtctaaac gcagagaacg ccatcaagaa attctgtcag agcaattgaa atctgatgtc
      301 agtaatagcc gtctgatgat tgccaaccct acgcacattg ccatagggat atactttaag
      361 ccacatcttt cacctatacc attgatttct gtgagagaaa ctaatgaggt agcattagcc
      421 gtaaggaaat atgcaaagga aatcggaata ccaattatta cagataaaaa attagcacga
      481 aaaatttatg ctacccatcg tcgctacgat tatgttagct tcgaaaatat agatgagatc
      541 ttgcgtcttc tgctgtggct tgaagatgtg gagaatgctg gacaacctgt tccagatgaa
      601 ctgctccc
//
LOCUS       DACBVI010000380          606 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.386, whole genome shotgun sequence.
ACCESSION   DACBVI010000380 DACBVI010000000
VERSION     DACBVI010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.386"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
BASE COUNT          225 a          123 c           98 g          160 t
ORIGIN      
        1 cccggcaaga atctctgatt gagttaagtg cccatcataa tatgatcaat gacattatat
       61 tacacaatca ccccatagtg gagaaaatca ctttaaacga caaccatatt gcacatttaa
      121 acgcgaaagc cactacaaaa ctggaatatt taaacttaag caataacaat ttattgccaa
      181 cagatgacat tgatcaatta atatcatcaa aacatctttg gcatgtatta gttaacggca
      241 tcaacaatga tccacttgcc caaatgcagt actggactgc agtaagaaat ataattgatg
      301 acactaatga agtgaccatt gatttatctt ataacctggc aatcacaaat atcgatacca
      361 gcgatgaaca tcttgtagaa gtaagcgaga attccgaagg aaatcatata aaagacaatg
      421 actcaatgtc tattcgttat agatcaaaat attattccag agagtacgat ttaatagaag
      481 aagaaacaat attttctgac gcagaactaa aagccattct gcctatgcat cgcatgtacg
      541 gggttggtga ctataagtca aattcctctt ctctaccctc acactcgggg ctaaaggacc
      601 caacgg
//
LOCUS       DACBVI010000381          591 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.388, whole genome shotgun sequence.
ACCESSION   DACBVI010000381 DACBVI010000000
VERSION     DACBVI010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.388"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..501)
                     /locus_tag="IU325_23365"
     CDS             complement(<1..501)
                     /locus_tag="IU325_23365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HAP5811904.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRVLVIVGND
                     VSLGRSDSFSELPENTIVIPYGELTGSFEVIITTDPDLVVTIRELTAQEPEDRTLTLN
                     QQIEVLGLKFAVKVKNEVWQYSIPGIVENNITSTRQHFFRSTLFKYVTLFFLFAIIFI
                     AFYIVNA"
BASE COUNT          206 a          112 c           87 g          186 t
ORIGIN      
        1 ggcattaaca atataaaaag ctataaaaat gatagcaaaa agaaaaaata gcgttacata
       61 tttaaacagc gtacttctaa agaaatgttg tcttgttgaa gtaatgttat tttcaacaat
      121 acctggtatt gaatactgcc aaacttcatt ttttactttt acagcaaatt tgaggcctaa
      181 aacttctatt tgctgattca atgttaacgt tctatcttca ggttcttgtg ctgttaattc
      241 tctgattgtt acaaccagat cagggtcggt agtgattatt acctcaaaac tgcctgtaag
      301 ctctccataa ggaatgacta tcgtattctc tggtaactca gaaaaagaat cacttcttcc
      361 taacgaaaca tcattaccaa cgataacgag tacacgccca ttaagtattt catactcgca
      421 tccattcaat ggtccgctca ataaacgaat ggcataagat tccaataagt cttgtgataa
      481 gaatttatca ttattttcca tgaataccac ccgctaattc cataagctct agtttagagt
      541 agtgtaacaa gacggaaatt tcatgaccaa attatgatga cacacaataa a
//
LOCUS       DACBVI010000382          586 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.389, whole genome shotgun sequence.
ACCESSION   DACBVI010000382 DACBVI010000000
VERSION     DACBVI010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.389"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            72..>586
                     /locus_tag="IU325_23370"
     CDS             72..>586
                     /locus_tag="IU325_23370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811905.1"
                     /translation="MVSVTSYTYPTETENDNSQFQSQTQSQGLSQSLRDSIEGNIAAF
                     DAMEQSGLLDNCRTLSKNASFREIFRSLLLYILDSLHIYRHDISDKLVSYFHVTPESA
                     NANYKTWIATTGMDSYIKIEQTGSDKFKVTDVYRELNGKSLEKKSRDRDFSGRNVKEF
                     IGLLSEHDVLE"
BASE COUNT          194 a           83 c          132 g          177 t
ORIGIN      
        1 atatttatca tgctaaccat tgatgcttct aaaatgtcaa aatatgcaaa gtgtcatatt
       61 gcgaggttac tatggttagt gtgacgagtt atacatatcc aacggaaaca gagaatgata
      121 atagccagtt tcagagtcaa actcagagtc agggcctgag ccagagtcta agagatagta
      181 tcgagggtaa tattgctgca tttgatgcga tggagcaaag cggcctgctc gataattgta
      241 gaacattgtc taaaaatgcc tcttttcgtg aaattttccg aagtttattg ttatacatct
      301 tagattcctt acatatctat cgacatgata ttagtgataa attggtttct tattttcatg
      361 ttacgcctga atcagcgaat gcgaattata aaacatggat cgctaccaca ggcatggata
      421 gctatataaa aattgagcag accggcagtg ataaatttaa agtcactgat gtgtatcgtg
      481 agttaaatgg caagagtttg gaaaaaaaga gtagggatag ggattttagt ggtcgaaatg
      541 tgaaagagtt tatagggtta ttatcagagc acgatgtatt ggaaaa
//
LOCUS       DACBVI010000383          583 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.390, whole genome shotgun sequence.
ACCESSION   DACBVI010000383 DACBVI010000000
VERSION     DACBVI010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.390"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            1..583
                     /locus_tag="IU325_23375"
                     /pseudo
     CDS             1..583
                     /locus_tag="IU325_23375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          133 a          152 c          181 g          117 t
ORIGIN      
        1 gcgtcggcgt cgtacaccgc aggaaaaaat tgccattatt cagcagacta tggagcccgg
       61 tatgaccgtg tctcatgtcg cgcgcctgca tggcatcaat gcaaaccaga tattcaaatg
      121 gcgcaagcaa tattaagacg gttcactgac ggctgttgca tcgggcgagg aagtggtgcc
      181 ggcctccgaa ctcgctgccg ccaataagca aattcgcgaa ctccagcgcc ttctgggcaa
      241 aaagacgatg gaagccgaga tactcaaaga ggccgtggag ttcggccggg caaaaaaatg
      301 gattgcgcat gcgccctcgt tgcctgggaa cgacgaataa gcgacgtttg cagaagtctt
      361 ggcgtgtcgc gtgcgcagtt gagcattcgg gttcaccggc catctgactg acaggatcgc
      421 agacggcagc cccgttctga cgataccacc gtattgtccc ggataaacat ggcggtggct
      481 gacctgccga cgtatggtta tcgccgtgtg tgggcgctgc tgcgacggga gtcggagcgg
      541 gacggcctgc ctgtggttaa tgcaaagcgg gtgtatcgca tta
//
LOCUS       DACBVI010000384          565 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.391, whole genome shotgun sequence.
ACCESSION   DACBVI010000384 DACBVI010000000
VERSION     DACBVI010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.391"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            224..433
                     /gene="fumD"
                     /locus_tag="IU325_23380"
     CDS             224..433
                     /gene="fumD"
                     /locus_tag="IU325_23380"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HAP5811906.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          173 a           90 c          135 g          167 t
ORIGIN      
        1 ccagggaaaa ggttacgcaa aaacttcgct gtttgcgcaa cgtctggtgc cggggttgtc
       61 actgtgaaga taagtgaaag ttcatccggt gtataaatac atcatcgctc aattgctaca
      121 gttaaaataa tttatcgcgt tatttgtctg attttgtttg atttttgtct aattggttag
      181 attgatatat gctgatagtt ggcatgataa ataggtataa agaatgggaa atcggacaaa
      241 agaggatgaa ctgtaccgag aaatgtgcag agttgtcggt aaagttgtgc tggaaatgcg
      301 cgatctgggg caggaaccaa agcatattgt tatcgcaggc gtattacgta cagcattagc
      361 caataaacgc attcagcgca gtgagctgga aaagcaggcg atggaaaccg tcattaacgc
      421 actggtaaaa tgaccattca ggatgaggcg ataagcttca tcctgatatt cttgcttcag
      481 ttgcggtcca gttaatcatt ctaaataaca actgctgaaa ttatacgaat ttcggtatgt
      541 attttaattt atagcgataa tgtta
//
LOCUS       DACBVI010000385          543 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.392, whole genome shotgun sequence.
ACCESSION   DACBVI010000385 DACBVI010000000
VERSION     DACBVI010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.392"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
BASE COUNT          177 a          121 c           84 g          161 t
ORIGIN      
        1 gacagtacgc attatattgt accccccact tattgataat aataaatacc cagatatgtt
       61 aaatcatctt gtcatacaac cacgctgatt ttgagaacac cagtcagctt tccctaactc
      121 tgcaaagagt gacctttctc atcaagctga tcaagattat ttttatctgc cacaaagtta
      181 ttaattacca gagccagagt accgtgcccc ttcgttgtag taacaaaaca gccctctatc
      241 ttctattttg tatatgattg tgaagattgg ctactaagct gtaacgcccc atgttcccta
      301 gaatagccta taaaatcata gcccaacagg ttctacagtt gaaaaacatt tacgttggca
      361 taacgtttca gaaaggacaa taagtgtacc gcagtctcag gatagattat gcagacctga
      421 cttgctcact aagcctcaaa tcacattgta gcaactttgt cgaaacatat tctgtagatt
      481 attagccagt acctgaggaa tctccctaaa aataaaatgc aatgtgatac ttttacaaat
      541 taa
//
LOCUS       DACBVI010000386          541 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.393, whole genome shotgun sequence.
ACCESSION   DACBVI010000386 DACBVI010000000
VERSION     DACBVI010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.393"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
BASE COUNT          205 a          104 c           73 g          159 t
ORIGIN      
        1 ttttatcatt cagttataaa aaaatgatcc tatgtttgaa catagcatta ctgtgataaa
       61 atataacatt ctctcttgat cgtataggaa aactatattc ataaaagcag cacttagtga
      121 ttgttcaaga caatcacatt gctggagcag ggtttatccc tgttagtgat ggatttatcc
      181 aaaaaagatc gcgtcgaaga cgcacacaaa gtcaaaggca aattaattaa tctctcacaa
      241 taaaagcagc gattcgtgat tgtagaaaac aatcactccg ctgtaataac agaataagaa
      301 aaataaacag attacaccat tggtgccatt ttcataaggc acaaataaac ttcatcgcaa
      361 aacgtttctg gacagatcaa ttaagaaata tttcaataca attcatcctt cattgctgaa
      421 aacctccaac gccattcata aaacagcagg tgccattcaa caggcacaca ttccctttta
      481 ctatattaaa cttattacta tattacagct gttatttctc atataaaaca atatattaca
      541 g
//
LOCUS       DACBVI010000387          539 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.394, whole genome shotgun sequence.
ACCESSION   DACBVI010000387 DACBVI010000000
VERSION     DACBVI010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.394"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..526
                     /locus_tag="IU325_23385"
     CDS             <1..526
                     /locus_tag="IU325_23385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAP5811907.1"
                     /translation="TAGSLKLGKVQVSVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGTLGLVTN
                     AVVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGH
                     LRPLKEASEAENVA"
BASE COUNT          145 a          131 c          141 g          122 t
ORIGIN      
        1 gaccgctggc tccctgaagc tgggcaaagt acaggtttca gtgctggtcc gttcattgct
       61 gaaaagtgaa cgtccttccg gactgactca ggcaatcatt gaagtggggc gcatcaacaa
      121 aacgctgtat ctgcttaatt atattgatga tgaagattac cgccggcgca ttctgaccca
      181 gcttaatcgg ggagaaagtc gccatgccgt tgccagagcc atctgtcacg gtcaaaaagg
      241 tgagataaga aaacgatata ccgacggtca ggaagatcaa ctgggcacac tggggctggt
      301 cactaacgcc gtcgtgttat ggaacactat ttatatgcag gcagccctgg atcatctccg
      361 ggcgcagggt gaaacactga atgatgaaga tatcgcacgc ctctccccgc tttgccacgg
      421 acatatcaat atgctcggcc attattcctt cacgctggca gaactggtga ccaaaggaca
      481 tctgagacca ttaaaagagg cgtcagaggc agaaaacgtt gcttaacgtg agttttcgt
//
LOCUS       DACBVI010000388          537 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.395, whole genome shotgun sequence.
ACCESSION   DACBVI010000388 DACBVI010000000
VERSION     DACBVI010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.395"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..>537
                     /locus_tag="IU325_23390"
     rRNA            <1..>537
                     /locus_tag="IU325_23390"
                     /product="16S ribosomal RNA"
BASE COUNT          136 a          119 c          179 g          103 t
ORIGIN      
        1 ggggaggaag ggagtaaagt taataccttt gctcattgac gttacccgca gaagaagcac
       61 cggctaactc cgtgccagca gccgcggtaa tacggagggt gcaagcgtta atcggaatta
      121 ctgggcgtaa agcgcacgca ggcggtttgt taagtcagat gtgaaatccc cgggctcaac
      181 ctgggaactg catctgatac tggcaagctt gagtctcgta gaggggggta gaattccagg
      241 tgtagcggtg aaatgcgtag agatctggag gaataccggt ggcgaaggcg gccccctgga
      301 cgaagactga cgctcaggtg cgaaagcgtg gggagcaaac aggattagat accctggtag
      361 tccacgccgt aaacgatgtc gacttggagg ttgtgccctt gaggcgtggc ttccggagct
      421 aacgcgttaa gtcgaccgcc tggggagtac ggccgcaagg ttaaaactca aatgaattga
      481 cgggggcccg cacaagcggt ggagcatgtg gtttaattcg atgcaacgcg aagaacc
//
LOCUS       DACBVI010000389          530 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.396, whole genome shotgun sequence.
ACCESSION   DACBVI010000389 DACBVI010000000
VERSION     DACBVI010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.396"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..>530
                     /locus_tag="IU325_23395"
     CDS             <1..>530
                     /locus_tag="IU325_23395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP5811908.1"
                     /translation="AIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIK
                     ETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFES
                     EVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIH
                     PIAMDDGLRFAIREGGR"
BASE COUNT          128 a          131 c          148 g          123 t
ORIGIN      
        1 gcgattgaca agccgttcct gctgccgatc gaagacgtat tctccatctc cggtcgtggt
       61 accgttgtta ccggtcgtgt agaacgcggt atcatcaaag ttggtgaaga agttgaaatc
      121 gttggtatca aagagactca gaagtctacc tgtactggcg ttgaaatgtt ccgcaaactg
      181 ctggacgaag gccgtgctgg tgagaacgta ggtgttctgc tgcgtggtat caaacgtgaa
      241 gaaatcgaac gtggtcaggt actggctaag ccgggcacca tcaagccgca caccaagttc
      301 gaatctgaag tgtacattct gtccaaagat gaaggcggcc gtcatactcc gttcttcaaa
      361 ggctaccgtc cgcagttcta cttccgtact actgacgtga ctggtaccat cgaactgccg
      421 gaaggcgtag agatggtaat gccgggcgac aacatcaaaa tggttgttac cctgatccac
      481 ccgatcgcga tggacgacgg tctgcgtttc gcaatccgtg aaggcggccg
//
LOCUS       DACBVI010000390          525 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.397, whole genome shotgun sequence.
ACCESSION   DACBVI010000390 DACBVI010000000
VERSION     DACBVI010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.397"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            322..>525
                     /locus_tag="IU325_23400"
     CDS             322..>525
                     /locus_tag="IU325_23400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811909.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAEKVLTQTT
                     SWVLNSRYVNVNSVSKVNVKSKVK"
BASE COUNT          184 a           73 c           95 g          173 t
ORIGIN      
        1 ctttttttac cataatgtta cttagtggca tcggcaattt acataactta catgttgttt
       61 tttatatcct gaaaaaacag atagatagtg tgcacactcg aaatgatatt ggtaacataa
      121 atagctttat tcgtgacaaa catctcaaaa gatttccttt attattttcg aaattcattt
      181 gatcaaggtt atcaccttat ggagacgtta cgtagaatga ctatatttgt tatcggcaca
      241 ttaggggtat taaatttagt attgatggaa ggtgattatt gaacagtaac atcgagtcac
      301 ggtcaaaatg tgaggtatat aatggacatt gagttttcgc agattcatga aatggtctat
      361 atgcatgata ttgtaaattc ggactcgaaa aagaaaccaa ggattccgtt aaaaaaattc
      421 cttaatgctg agaaagttct cacgcaaaca acatcatggg tactaaattc aagatatgtg
      481 aatgttaatt ctgtcagtaa agtcaacgta aagagtaaag ttaaa
//
LOCUS       DACBVI010000391          512 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.398, whole genome shotgun sequence.
ACCESSION   DACBVI010000391 DACBVI010000000
VERSION     DACBVI010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.398"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(83..457)
                     /locus_tag="IU325_23405"
     CDS             complement(83..457)
                     /locus_tag="IU325_23405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HAP5811910.1"
                     /translation="MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGASSQLINSIDILRARRATG
                     LMTRHNYRTVNNITLGKYPEAK"
BASE COUNT          116 a          128 c          135 g          133 t
ORIGIN      
        1 ccatcaatgt caacgacaat tcgccccata ttcagtgcct gtacgttaac actgtcggct
       61 tccagtgtca gggccagttt catcatttcg cctccggata cttacccagg gtgatattgt
      121 tcaccgttct gtagttgtgg cgggtcatca ggcctgttgc ccggcgagcc cggagaatat
      181 caatgctgtt gattaactgc gacgaggctc ctgcgctgaa tcccggctgg tcagttcgta
      241 ccagtgcgta tttttccacg agaaagttca ctgcatcaca cagtgaaatg cctgcctcaa
      301 tatgctgctc aatcacgcgt tcatccacga acggtgtgtc attcagcgta aggctgtaat
      361 gctggtccag taaccgggtg agcagtgtct gccagatggt gacgggagac gggcagcacg
      421 atacctcccg tacatgcgtg tcgggtaatg ttttcatact gaagattttc ctgatatgca
      481 gagataaaaa atgggacagt ggcgtggtga aa
//
LOCUS       DACBVI010000392          510 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.399, whole genome shotgun sequence.
ACCESSION   DACBVI010000392 DACBVI010000000
VERSION     DACBVI010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.399"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..>510)
                     /locus_tag="IU325_23410"
     CDS             complement(<1..>510)
                     /locus_tag="IU325_23410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811911.1"
                     /translation="DYSRRYAISENIAKDAVTNNIALMSTFYDSDNTSGEVLNNDFTE
                     EESFETALDVEHAIVLGFADDNFETKPVHYQDLLTRFSAFQDTVFNLFPEMHSSHYND
                     ICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMVREDATNLVNLKTLLNDGCFIKY
                     SHFNDVALIK"
BASE COUNT          164 a          106 c           75 g          165 t
ORIGIN      
        1 ctttattaat gccacgtcat taaaatgact atatttaata aagcaaccat cattgagtag
       61 tgttttaaga ttgactaaat tagttgcatc ttcacgtacc atcgtggcaa ctggaacagg
      121 caacgacatg aaaacttctt cattcaccat caagtggatc atgcattgcg ttccctttgt
      181 catatccaca cttacgctac aaatatcgtt gtaatgcgaa gagtgcattt caggaaacaa
      241 attaaacacg gtatcttgaa aggcactaaa tcgtgttaat aaatcctgat aatgaacagg
      301 ttttgtttca aaattatcat cagcaaatcc aagcactatt gcatgctcta catctaacgc
      361 agtttcaaat gactcttctt ctgtaaaatc attatttaaa acctcacctg acgtattatc
      421 actgtcataa aaagtgctca tcaatgcgat attgtttgtt acagcgtctt ttgcgatatt
      481 ctcactaatt gcatatcgcc tgctataatc
//
LOCUS       DACBVI010000393          505 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.400, whole genome shotgun sequence.
ACCESSION   DACBVI010000393 DACBVI010000000
VERSION     DACBVI010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.400"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..>505
                     /gene="eilA"
                     /locus_tag="IU325_23415"
     CDS             <1..>505
                     /gene="eilA"
                     /locus_tag="IU325_23415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HilA family transcriptional regulator EilA"
                     /protein_id="HAP5811912.1"
                     /translation="IIDKVWSASVASDESLTRCIYALRKLLHENKQCKYIETVYGRGY
                     RFTVPIVIVTDNEPVKSTTTLAVFPFRTEGSINVLKLHYELVQGLSKYAFCGLDILPA
                     SVTNEASDFSTIHQFINKTGPEYYIMGQVVHYGKNWRLFIELIHARTHKLIDHQSIDF
                     NPDNPLSV"
BASE COUNT          142 a           97 c           97 g          169 t
ORIGIN      
        1 gattatcgat aaggtctgga gcgcgagtgt tgccagtgat gaatctctaa cacgatgtat
       61 ttatgcattg cgcaaacttt tgcatgagaa taaacagtgt aagtatatcg aaacggtcta
      121 tggtcgtgga taccgtttta ctgtacctat tgtgattgtg accgataatg aaccggtaaa
      181 gtcgaccact actcttgccg tttttccctt tcgtactgaa ggttcgatca atgttctaaa
      241 actccattac gaacttgtcc agggcctttc aaaatatgct ttttgtggtt tagatatttt
      301 acccgcttcg gtaacaaatg aggccagtga tttttccact atccatcagt ttatcaataa
      361 aacaggtcca gaatattata ttatgggtca ggtcgttcac tatggtaaaa actggcgttt
      421 atttattgag ttaattcatg ccagaacaca taaattgatt gatcatcaaa gtattgattt
      481 caaccccgat aatcctcttt ccgta
//
LOCUS       DACBVI010000394          487 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.401, whole genome shotgun sequence.
ACCESSION   DACBVI010000394 DACBVI010000000
VERSION     DACBVI010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.401"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            3..>487
                     /locus_tag="IU325_23420"
     CDS             3..>487
                     /locus_tag="IU325_23420"
                     /inference="COORDINATES: protein motif:HMM:NF024977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811913.1"
                     /translation="MDAARDWLIANKCNRGKDNLPLTYDGFYVCIRIDEDSIRVLDNC
                     AGIDRETLTKRALYTNKPSQHEYGIGLYGIGLKRSLLKMGTDFSFYVDDGIEGFKSRF
                     NNRGIGGGKEDPVYADVIPTKNRRKSLFNVSNLKNEIKNDLHNNRWFTNAVSEFSLRY
                     A"
BASE COUNT          167 a           62 c          105 g          153 t
ORIGIN      
        1 ctatcgatgc ggcgagagac tggttaatag caaataaatg taatagagga aaagataatt
       61 tacctctaac ttatgatgga ttctatgttt gcataaggat agatgaagat agtattcgag
      121 ttcttgataa ttgcgctggt attgatagag aaactcttac caaaagagca ttatatacga
      181 ataaaccatc tcagcacgag tatggcattg gattatatgg aataggttta aaacgctcat
      241 tgctaaaaat gggtactgat ttttcttttt atgttgatga tggtattgaa ggtttcaagt
      301 cgaggtttaa taatcgaggg atcggaggtg gtaaggaaga cccggtctat gctgatgtta
      361 ttcctacaaa gaatagaagg aagtcactat ttaacgtttc caacttaaaa aacgaaataa
      421 aaaatgatct tcataataat agatggttca ctaatgccgt aagtgaattt tctttgaggt
      481 atgctat
//
LOCUS       DACBVI010000395          472 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.402, whole genome shotgun sequence.
ACCESSION   DACBVI010000395 DACBVI010000000
VERSION     DACBVI010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.402"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..>472
                     /locus_tag="IU325_23425"
     rRNA            <1..>472
                     /locus_tag="IU325_23425"
                     /product="16S ribosomal RNA"
BASE COUNT          118 a          113 c          142 g           99 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg ga
//
LOCUS       DACBVI010000396          465 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.403, whole genome shotgun sequence.
ACCESSION   DACBVI010000396 DACBVI010000000
VERSION     DACBVI010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.403"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..>465
                     /locus_tag="IU325_23430"
     rRNA            <1..>465
                     /locus_tag="IU325_23430"
                     /product="23S ribosomal RNA"
BASE COUNT          133 a          104 c          139 g           89 t
ORIGIN      
        1 gttaagcgac taagcgtaca cggtggatgc cctggcagtc agaggcgatg aaggacgtgc
       61 taatctgcga taagcgtcgg taaggtgata tgaaccgtta taaccggcga tttccgaatg
      121 gggaaaccca gtgtgattcg tcacactatc attaactgaa tccataggtt aatgaggcga
      181 accgggggaa ctgaaacatc taagtacccc gaggaaaaga aatcaaccga gattccccca
      241 gtagcggcga gcgaacgggg aggagcccag agcctgaatc agtgtgtgtg ttagtggaag
      301 cgtctggaaa ggcgcgcgat acagggtgac agccccgtac acaaaaatgc acatactgtg
      361 agctcgatga gtagggcggg acacgtggta tcctgtctga atatgggggg accatcctcc
      421 aaggctaaat actcctgact gaccgatagt gaaccagtac cgtga
//
LOCUS       DACBVI010000397          464 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.404, whole genome shotgun sequence.
ACCESSION   DACBVI010000397 DACBVI010000000
VERSION     DACBVI010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.404"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            70..276
                     /locus_tag="IU325_23435"
     CDS             70..276
                     /locus_tag="IU325_23435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811914.1"
                     /translation="MEVNGDGDANDYLDDQEFMSPFIIYVIDHIQNNFERFTVQEGNS
                     GDINQVDFELVAAMLVEYSECFRK"
BASE COUNT          163 a           70 c           95 g          136 t
ORIGIN      
        1 agtaataaat caactgaact gttctatgat cttgcctgca ggtcgtttac cgcaagctgg
       61 aatatgttca tggaagtcaa tggtgatggg gatgccaatg attatctgga tgatcaagag
      121 tttatgagcc catttattat ttatgttatt gaccatattc agaacaactt tgaaagattc
      181 actgtccaag aaggaaatag tggtgatatt aatcaagttg actttgaact agtcgctgcc
      241 atgttagttg agtattcaga atgttttaga aaataagaac caatcttgcc ccacaacggg
      301 ccaaaccaaa acataaaaag ggaaatataa atggcaaata caatatctgt cgcagtaaat
      361 atgttttgtg aagaaactcc aaaagtaatg aatgctattc aagagcgtta tgaaatattt
      421 gttgccatta cgggatattc agtagaagaa atcatggatg acag
//
LOCUS       DACBVI010000398          460 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.405, whole genome shotgun sequence.
ACCESSION   DACBVI010000398 DACBVI010000000
VERSION     DACBVI010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.405"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
BASE COUNT          120 a           98 c           79 g          163 t
ORIGIN      
        1 aaaaaattat aaatcatgaa gaagccaaag ctctttttat ggtttctttt ttagctcgcg
       61 ttgcggataa ggaaacgcca aaacactttc acctatattg tttttagata cggcattcca
      121 ccatttttcg cgatattctg gatgattaca gtagtctcgc tgccgttgaa tttcatcaag
      181 aatatggtct ttttggtcag gccattcttc ttgcaatctg tttatcgttc tgtagaaaat
      241 attcgactgc atcattattt tgaagaagtt aagcctaaac ccttccagac gttgctcgta
      301 atcagcgaca accttcttat ggctattgat tgtgttattc aactgcatct gtattctact
      361 aaccctgtca ttagccatag acgcttcaaa ctgcgactgt tgagaatcca gccttgcctc
      421 atttgcctgt tggtaagagt ctacaatttc tctattacgt
//
LOCUS       DACBVI010000399          458 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.406, whole genome shotgun sequence.
ACCESSION   DACBVI010000399 DACBVI010000000
VERSION     DACBVI010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.406"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..231
                     /locus_tag="IU325_23440"
     CDS             <1..231
                     /locus_tag="IU325_23440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP5811915.1"
                     /translation="IKDERDLLDNPCLNIKIGTEILYNHFSRCGVTWQCLGTYNAGFA
                     MENKKKRLQYAKKIYVVYTRLNEIDKRKALAK"
     gene            complement(250..>458)
                     /locus_tag="IU325_23445"
     CDS             complement(250..>458)
                     /locus_tag="IU325_23445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HAP5811916.1"
                     /translation="SSDNQLTNRESEILQLIADGKTNKEIANFLQLSRKTVETHRLNI
                     MKKLDVHSGIELIKTALRMGVCTI"
BASE COUNT          152 a           72 c           86 g          148 t
ORIGIN      
        1 ataaaagatg aacgagattt gttagataac ccctgtctga acattaaaat agggaccgag
       61 attttatata atcatttttc acgttgtggg gtaacgtggc aatgtctggg gacatataac
      121 gcgggttttg caatggaaaa caaaaaaaag aggctgcaat atgcaaaaaa aatatatgtt
      181 gtatatacca gacttaatga aatagacaag cgtaaggctt tagcaaaata aacctaaagc
      241 ctcttttttt tatatagtgc aaacacccat acgtaaagct gttttgatca actcgatacc
      301 actgtgaaca tctaatttct tcatgatatt gagtctatga gtttcgactg ttttcctgct
      361 taactgcaag aaattagcga tttctttgtt tgttttccca tcagctatga gttgtagtat
      421 ttcagactca cgatttgtaa gttgattatc agaggaat
//
LOCUS       DACBVI010000400          458 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.407, whole genome shotgun sequence.
ACCESSION   DACBVI010000400 DACBVI010000000
VERSION     DACBVI010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.407"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            34..402
                     /locus_tag="IU325_23450"
     CDS             34..402
                     /locus_tag="IU325_23450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811917.1"
                     /translation="MENNNERSSMEIFEKDQKIAFVDSFSDAVLSYEYEQAGFTINLM
                     QRSVLLFNHNIKKNVMSSTLRKNMISTFIYPYSNIREINYSLPDCRSDDAEICIMTDD
                     VLNPVWTFRVPSHAHYICEQ"
BASE COUNT          157 a           86 c           86 g          129 t
ORIGIN      
        1 tgagataaag cagtaaaaca gcgagggatg agcatggaaa acaacaacga gcgaagctct
       61 atggaaatct tcgaaaaaga tcaaaaaatc gcttttgttg actcgttcag cgatgctgtt
      121 ttgtcttacg aatacgaaca ggcgggcttc accattaatt taatgcaacg ctcggttctt
      181 ctctttaacc ataatataaa gaaaaacgta atgtcttcga cattgcgtaa aaatatgatc
      241 agcacattta tatatccata tagcaacatc agggaaatca actactcgct gcctgattgc
      301 agatccgatg atgccgagat ctgtattatg actgatgatg tattaaatcc agtctggacg
      361 ttcagggttc catctcacgc acattatatt tgcgagcaat gagtggaagt atttaataac
      421 catattttct ttgactcata aacaaagtat taaacaaa
//
LOCUS       DACBVI010000401          456 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.408, whole genome shotgun sequence.
ACCESSION   DACBVI010000401 DACBVI010000000
VERSION     DACBVI010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.408"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..>456)
                     /locus_tag="IU325_23455"
     CDS             complement(<1..>456)
                     /locus_tag="IU325_23455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811918.1"
                     /translation="KLGKYIFSSRAPQVLELAIGWRGALSESIKGVKFCIWFSVAWRT
                     IEFIMSSERDLVNFLGDFSMDVAKAVIAGGVATAIGSLASFACLSFGLPVILVGGAIL
                     LTGIVCTVVLNEIDAQYHLSEKLKDAIRDGLKRQQELDKWKRENMTPFM"
BASE COUNT          145 a          106 c           71 g          134 t
ORIGIN      
        1 acataaatgg agtcatgttt tcccttttcc atttatcaag ttcctgttgc cgttttagtc
       61 catctctaat tgcatctttt aatttttctg ataaatggta ttgagcatcg atttcattta
      121 aaacaactgt acacactatc cctgtaagta aaattgctcc tcctacaaga attactggaa
      181 ggccaaaact aagacaggca aaagaagcca ggcttccaat agcagttgca acaccgcctg
      241 caatcactgc tttggccaca tccattgaaa aatcaccaag aaaattaacc agatctcgct
      301 ctgaactcat aataaattct atggttctcc aggctactga aaaccagata cagaacttaa
      361 ctccttttat tgattcagat aaagctcctc gccagccaat agcgagttcc agaacttgag
      421 gcgctcgtga agagaaaata tatttaccga gctttt
//
LOCUS       DACBVI010000402          447 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.409, whole genome shotgun sequence.
ACCESSION   DACBVI010000402 DACBVI010000000
VERSION     DACBVI010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.409"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..>447
                     /locus_tag="IU325_23460"
     CDS             <1..>447
                     /locus_tag="IU325_23460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HAP5811919.1"
                     /translation="SAIHHQIIENQLDTETLTVSYEFILCQHLIANTEFSYLALPENY
                     NCLFLPNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQ
                     ILNEKTAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKLLDGI"
BASE COUNT          166 a           78 c           79 g          124 t
ORIGIN      
        1 tcggctattc atcatcagat cattgaaaat caactggata cagaaacatt aactgttagt
       61 tatgaattta tcctctgtca acatcttatc gcaaataccg agttcagcta tttggcactc
      121 cctgaaaatt ataactgttt gtttttacca aattcaaaaa accaaacaaa taatcgtttc
      181 aaaacgttaa acagcaaagc tattggcagg ctacttgctg ctggtggcgt atacaatggg
      241 aacattgaag gattcagaga tactgcggaa aaactgggtg gagatgcaat aaaaggctat
      301 gatcaaatac taaatgaaaa aacagcgggc atagcgatag caacagcatc tattctttta
      361 acaaagcgtt ctaatgttga tacatataca gaaataaata gttacttagg caaacttaga
      421 ggtcaacaaa aacttcttga tggtata
//
LOCUS       DACBVI010000403          425 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.410, whole genome shotgun sequence.
ACCESSION   DACBVI010000403 DACBVI010000000
VERSION     DACBVI010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.410"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..>425)
                     /locus_tag="IU325_23465"
     CDS             complement(<1..>425)
                     /locus_tag="IU325_23465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811920.1"
                     /translation="LMDKDAQGYTDLSDLDLTSCHFKGDVISKVSFLSSNLQHVTFEC
                     KEIGDCNFTTATVDNVIFKCRRLHNVIFIKASGEYVDFSQSILDTVDFSRSQLTHSNF
                     RECQIRNSKFNNCYLYASHFTRAEFLSDKEISFIKSNLT"
BASE COUNT          148 a           80 c           65 g          132 t
ORIGIN      
        1 ctgtcaaatt cgatttaata aatgatattt ctttgtcaga aagaaattct gctctggtga
       61 agtgcgaagc ataaagataa caattattga actttgaatt tctaatctga cattcacgaa
      121 aattactatg agtaagctgg ctccgcgaga agtcaactgt atcaagaata ctttggctaa
      181 aatcgacata ttcgccactc gctttgataa aaatcacatt gtgtaaacgt cgacatttaa
      241 atatgacatt atcaactgtt gcagtagtaa aattgcaatc ccctatttct ttacattcga
      301 atgttacatg ttgtagattt gacgataaaa aagacacctt cgaaataacg tcacctttaa
      361 aatgacaact tgttaaatcc aaatcagaca ggtcagtata cccctgcgca tctttatcca
      421 tcaga
//
LOCUS       DACBVI010000404          423 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.411, whole genome shotgun sequence.
ACCESSION   DACBVI010000404 DACBVI010000000
VERSION     DACBVI010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.411"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..>423)
                     /locus_tag="IU325_23470"
     CDS             complement(<1..>423)
                     /locus_tag="IU325_23470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP5811921.1"
                     /translation="LDSAVMYMNGRMEMYRYTPESLKRALVIFRDCVSTQPQNTLPYC
                     CLAECHISLALLGLSEQKQAITTAVTSIEKALEINPSNSQALGLLGLISGLKDEHSVS
                     NVLFKQAHLLKPNSPDVYYYQSLLCFLNGDLARAFNLI"
BASE COUNT          129 a           83 c           81 g          130 t
ORIGIN      
        1 tctattagat taaatgctct tgcaaggtca ccgttcaaaa aacatagcag agactgataa
       61 taataaacgt ctggcgaatt aggtttgagt aaatgtgctt gcttaaatag cacattagac
      121 actgaatgtt catcttttag cccactgatt aaacccagta aacccaatgc ctgagaattt
      181 gaagggttaa tttcaagagc cttttcaatg gaggttactg ccgtagttat tgcttgtttt
      241 tgttcactga gtccaaggag ggctaatgat atatggcatt ccgccaggca acagtagggc
      301 aaggtatttt gcggttgcgt actcacacaa tctctaaata ttactaatgc tcgtttcaaa
      361 ctctctggtg tataacgata catttccatt ctgccattca tatacataac agcagaatcc
      421 aga
//
LOCUS       DACBVI010000405          422 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.412, whole genome shotgun sequence.
ACCESSION   DACBVI010000405 DACBVI010000000
VERSION     DACBVI010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.412"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..>422
                     /locus_tag="IU325_23475"
     CDS             <1..>422
                     /locus_tag="IU325_23475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP5811922.1"
                     /translation="KIKGLEQLLYLAAEREEFHVHIIGEGTYRGKLTNIIDKFDLSQR
                     VTLHGYISNASSMLGKFDVYVQPSKSEGFGIAVIEALVNKIPTVCSDIEVFRELFGSG
                     EVEFFKLDDINSLYDAISSALTKTDMSRDASATAISQK"
BASE COUNT          140 a           64 c           91 g          127 t
ORIGIN      
        1 aaaaataaaa ggcttagagc aactcctata tctagccgcg gagcgagaag agttccatgt
       61 tcatataatt ggtgaaggaa cttaccgtgg gaaattaaca aatattattg ataagtttga
      121 tttatctcag cgtgtaactt tgcatggata tatatcaaat gcgagttcta tgttggggaa
      181 atttgatgtt tatgtgcaac catctaaaag tgaggggttt ggcattgcag tcattgaagc
      241 tcttgtaaat aaaatcccaa cagtttgctc agacattgaa gtgttcagag agttatttgg
      301 ctcaggagaa gttgaatttt tcaagttaga cgatattaat tcactttacg atgcaatttc
      361 aagtgcatta accaagacag atatgtctcg agatgcaagt gccacagcga taagccaaaa
      421 at
//
LOCUS       DACBVI010000406          410 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.414, whole genome shotgun sequence.
ACCESSION   DACBVI010000406 DACBVI010000000
VERSION     DACBVI010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.414"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..>410)
                     /locus_tag="IU325_23480"
     CDS             complement(<1..>410)
                     /locus_tag="IU325_23480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HAP5811923.1"
                     /translation="DRFQRQIDFFMANPELDVVGGLCQEFGGESAREKCKIYFTDEAI
                     KNNAFKKCPFVHPTVMFKSSVFNNGMRYPENQPLTEDMCLWLELISANKKFANIDSVL
                     LKYRINEQTLKRRRGLNKAVTELSTRLYYAKKLK"
BASE COUNT          118 a           84 c           64 g          144 t
ORIGIN      
        1 ttttaagttt cttggcgtaa tacaatcttg tggataattc ggttacagcc ttattcaaac
       61 ctcttcttct ttttagcgtc tgctcattaa ttctgtactt tagaagaacg gagtcaatat
      121 ttgcaaactt tttattcgcg gatattaact cgagccataa gcacatatct tctgtcagtg
      181 gctgattttc agggtatcgc atcccgttat taaatacaga cgatttaaac atcacggttg
      241 gatgaacgaa aggacatttt ttaaaggcat tgtttttaat ggcttcatct gtaaaataga
      301 tcttgcattt ttctcttgct gactcaccac caaactcttg acacaaccca ccaacaacat
      361 ctaactcagg gttagccata aaaaaatcta tttgtcgttg aaatctatct
//
LOCUS       DACBVI010000407          409 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.415, whole genome shotgun sequence.
ACCESSION   DACBVI010000407 DACBVI010000000
VERSION     DACBVI010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.415"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
BASE COUNT          139 a           73 c           74 g          123 t
ORIGIN      
        1 tgataaacgt attgctgaaa actacgtctt tgacgaggta ttaggccgtc atattactcg
       61 aactaaattt gagaaaatgt ctagtttcaa ttgtcaaaat gacgaacaac cacctaatga
      121 gaatcagcgt tttccggatc gtcatctcaa ccctatacct cctgtaaatg attttgaatg
      181 cacaccaaca agcccggaag ataggaaatc agtatctaaa ttgaaaccac caagatattg
      241 ataagattgt aggtatgtat gaggacagcg cctgcaatac taattatttg gtggggaatc
      301 ctcattaaaa atgtatttgt tgatgaaaac aatcatttct tctttcagct ttacattatt
      361 tagagaaacg taatagtgat aggcctataa aattggatat tcaccggct
//
LOCUS       DACBVI010000408          407 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.416, whole genome shotgun sequence.
ACCESSION   DACBVI010000408 DACBVI010000000
VERSION     DACBVI010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.416"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            93..284
                     /locus_tag="IU325_23485"
     CDS             93..284
                     /locus_tag="IU325_23485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HAP5811924.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          102 a          105 c           78 g          122 t
ORIGIN      
        1 ttttttacat aacgaagaat tgaccatttt gtcctgttgt gccttaatgt aagtaccgtc
       61 cacagcgtgg gacatacttc aaggaacctt ttgtgagtca ggcaaccagt atgcgaaaac
      121 gacaccgatt taacagtcgc atgacccgta tcgtactgct catcagcttt atcttcttct
      181 ttggccgttt tatctactcg tccgtcggtg catggcagca ccatcagagc aaaaaagaag
      241 ctcagcaatc cacactctcc gtcgaatcac cggtacaacg ttagcggtta ccttctccac
      301 tttcacagaa cataacggca cctcgctgtc ggatgctttt gctctttggg attatcaaag
      361 cggcagatat tctttcatct taaattttac gtctttgtcc tgacaga
//
LOCUS       DACBVI010000409          399 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.417, whole genome shotgun sequence.
ACCESSION   DACBVI010000409 DACBVI010000000
VERSION     DACBVI010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.417"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
BASE COUNT          124 a           86 c           57 g          132 t
ORIGIN      
        1 catttcttct taacatattt cactctatga aagataaaac tgatacccac ctacagtttt
       61 gggtcaccaa caaaaaacaa tgttcgccag atattccacc actggcacag ttcaaccctg
      121 cttcaaaaat tgacttattc attgtgctca ttttcgtagt ccgttcaggt tgaataaaac
      181 tcccttcgtc aaatgaggaa agactgagag gttattactt tagcttacgt tatagctgtt
      241 ttcctttgca gtatttcttt gcttttctgt atacctttat acctgttata ccagatcaaa
      301 aaacaagcaa tccacataac aaaacgcgtt ttgttactga tgtcacaaat tgagcatatt
      361 tttgtagctg atagtttgtt acaaacacca gaaccaaga
//
LOCUS       DACBVI010000410          389 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.418, whole genome shotgun sequence.
ACCESSION   DACBVI010000410 DACBVI010000000
VERSION     DACBVI010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.418"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..>389
                     /locus_tag="IU325_23490"
     CDS             <1..>389
                     /locus_tag="IU325_23490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAP5811925.1"
                     /translation="ERKPADPCRKRAHKGRVAVAESNRRWCSDGFEFRCDNGEKLRVT
                     FAQDCCDREIIDWAASTGGYDKETVQDVMLGAVEKRFGRQLPTVPAFARLLGLEPCTT
                     AVRSPESNGIAESFVMTIKRDYISIRI"
BASE COUNT           97 a           90 c          131 g           71 t
ORIGIN      
        1 tgaacgtaaa cccgctgatc cttgccgtaa gcgggcccat aaggggcgcg tagcagtagc
       61 ggaaagtaac cggcgctggt gctcagatgg ctttgagttc cgctgtgata atggcgaaaa
      121 gctgcgggtg accttcgcgc aggactgctg cgacagggag atcattgact gggcagcgag
      181 caccggaggc tatgacaaag aaacggtgca ggatgtcatg ctgggtgcgg tggaaaaacg
      241 cttcggaagg cagttgccaa cagtgccggc gttcgcccgg ttgctgggac tggagccttg
      301 tacgacagca gtgcgaagcc cggaaagcaa tggcatagca gaaagcttcg tgatgacgat
      361 aaaacgggac tacatcagca tcaggattt
//
LOCUS       DACBVI010000411          385 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.419, whole genome shotgun sequence.
ACCESSION   DACBVI010000411 DACBVI010000000
VERSION     DACBVI010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.419"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
BASE COUNT          150 a           68 c           44 g          123 t
ORIGIN      
        1 cgattacgct ttctttgatg acgaaatgcc atttacatta actgatgaac agatcgaaaa
       61 tatttctttt ttaaacgcat atcacaaaga aaagaaagga aacaacgaca ttgttacatt
      121 tgatttcatg agcgctgatc accaccataa ttttagcaca accatagctt taacaaatga
      181 tagcttccat atatcttcaa taaacaacca taacgcacag gttatattcg atgaaaacat
      241 ccatttacat ccctatgaat taccagaatc atcacaatgg tgttatcagt taataaaaaa
      301 catgatttct ttacattgca gatataataa caatttcaaa ataaaaaaaa cctgttgttt
      361 tgattttaaa aataaatctt tttgt
//
LOCUS       DACBVI010000412          385 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.420, whole genome shotgun sequence.
ACCESSION   DACBVI010000412 DACBVI010000000
VERSION     DACBVI010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.420"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(167..>385)
                     /locus_tag="IU325_23495"
     CDS             complement(167..>385)
                     /locus_tag="IU325_23495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811926.1"
                     /translation="FYIDIDNDHSSCVYQGKNKACAMKPTLLSVVLAHEMGHAKGMKD
                     DGTDSMANVDKYENPFRKELGLPARMKY"
BASE COUNT          132 a           77 c           61 g          115 t
ORIGIN      
        1 tagtgacagg ggaatatgtt tggatgcgaa aaggcatcct ataatggaaa acatcagcta
       61 tacagcaaga tgaaaaaaat acggaaataa aatacagaca tactttcata ccatctccta
      121 aactggtgat atattaaaaa catacccaaa agcaatataa aaatgtttaa tatttcattc
      181 ttgctggcaa gcccaactct ttacgaaatg ggttttcata tttatcaaca ttagccatcg
      241 agtcagttcc atcgtctttc attccctttg catgccccat ttcatgcgca agaaccacac
      301 tcaacagagt gggtttcatg gcacatgctt tgttttttcc ttgatataca catgagctat
      361 ggtcgttatc aatatcaatg taaaa
//
LOCUS       DACBVI010000413          385 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.421, whole genome shotgun sequence.
ACCESSION   DACBVI010000413 DACBVI010000000
VERSION     DACBVI010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.421"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..>385
                     /locus_tag="IU325_23500"
     CDS             <1..>385
                     /locus_tag="IU325_23500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811927.1"
                     /translation="IHTVTKSDENGISKPTDLFTWHGKDKKYNTLAIVIKNTNGNDYL
                     SLGYYDQDNYHIKRGIRINGDSLTQYCSENARNASASFESNKALMAELFATGINHQVV
                     NELNGERLKEPNEVFKRYGRAIRYN"
BASE COUNT          152 a           68 c           70 g           95 t
ORIGIN      
        1 tcatacatac ggtaacaaaa tccgatgaga acgggataag caaaccaacg gacttattta
       61 cctggcacgg taaagataaa aaatacaaca ctctcgccat tgtaattaaa aatacaaacg
      121 gaaacgatta tttatctctc ggttactacg atcaggacaa ctaccatatt aaaagaggaa
      181 ttcgtattaa tggtgatagt ctcacacaat attgtagtga aaacgccagg aatgcttcag
      241 cgtcatttga aagcaataaa gctctcatgg cagaattatt tgcaacaggt ataaatcatc
      301 aagttgtaaa cgaactcaac ggggaaagac tgaaagaacc aaacgaagtt tttaaacgtt
      361 atggtcgagc aataagatat aattt
//
LOCUS       DACBVI010000414          370 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.423, whole genome shotgun sequence.
ACCESSION   DACBVI010000414 DACBVI010000000
VERSION     DACBVI010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.423"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            11..>370
                     /locus_tag="IU325_23505"
     CDS             11..>370
                     /locus_tag="IU325_23505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAP5811928.1"
                     /translation="MKFPSIFNKINPQSIQQHPEKNELNWMRELNKWKAERILTGEIH
                     RPECRNEAAKRINCAFLSKQNDIDLSGLNLTTQPPGLQNFTSINLDENQLKHFDATTY
                     DRLINLSLNSNALESINF"
BASE COUNT          139 a           78 c           57 g           96 t
ORIGIN      
        1 cggagcagtt atgaaattcc cttcaatatt taataaaata aatccacaat ccatacagca
       61 acatccagaa aaaaatgaac ttaactggat gcgcgaatta aataaatgga aagcagaacg
      121 catacttaca ggtgaaatcc atcgtccgga atgccgaaac gaagccgcta aaaggataaa
      181 ttgtgctttt ttgtcgaaac agaatgatat tgatttatca gggcttaatt taactaccca
      241 accaccagga ctgcaaaact tcacctctat caatcttgat gagaaccaac tcaaacattt
      301 tgatgcaacc acctacgata gactcatcaa ccttagtctg aatagtaatg ctcttgagtc
      361 aataaatttt
//
LOCUS       DACBVI010000415          369 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.424, whole genome shotgun sequence.
ACCESSION   DACBVI010000415 DACBVI010000000
VERSION     DACBVI010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.424"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
BASE COUNT          139 a           73 c           57 g          100 t
ORIGIN      
        1 atttatataa tttactgatg aaaaattctg ttctttaggg aagttaaaga ttatggcaga
       61 cctcagcata aaaccaggcc ctttaatacc ttagtatttg tgatctatag tttacagaca
      121 tgacagaata tgagcgaaca caactaattc agcaactatt gcgtccaccg tatctcgcag
      181 atacactaat aatcagtcag gatgccagca aagccgaaat taaatatctg attaccgata
      241 tcaattttaa cgctagtggg aacagaccac taaaaatgga tatcaacgct ctcatctgga
      301 ctttaaaaat atcgcaacac cgagtaattt caacacaaaa aaaattatac tgataacgca
      361 cttacgaag
//
LOCUS       DACBVI010000416          367 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.426, whole genome shotgun sequence.
ACCESSION   DACBVI010000416 DACBVI010000000
VERSION     DACBVI010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.426"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..>367
                     /locus_tag="IU325_23510"
     CDS             <1..>367
                     /locus_tag="IU325_23510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAP5811929.1"
                     /translation="DGRRAVLRSQVLELHGISHGSAGARSIATMATRRGYQMGRWLAG
                     RLMKELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVTEPNQVWCGDVTYIWTGKRW
                     AYLAVVLDLFARKPVGWAMS"
BASE COUNT           82 a           95 c          116 g           74 t
ORIGIN      
        1 cagacggcag acgggctgta ttacgcagtc aggtacttga gctacatggc atcagccacg
       61 gttcggccgg agcaagaagc atcgccacaa tggcaacccg gagaggctac cagatgggac
      121 gctggcttgc tggcaggctc atgaaagagc tggggctggt cagctgtcag cagccgactc
      181 accggtataa acgtggtggt catgaacatg ttgctatccc taactacctt gaaaggcagt
      241 tcgccgtgac cgagccaaat caggtgtggt gcggtgatgt gacctatatc tggacgggta
      301 agcgctgggc gtacctcgcc gttgttctcg acctgttcgc aagaaaacca gtgggctggg
      361 ccatgtc
//
LOCUS       DACBVI010000417          357 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.427, whole genome shotgun sequence.
ACCESSION   DACBVI010000417 DACBVI010000000
VERSION     DACBVI010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.427"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            2..>357
                     /locus_tag="IU325_23515"
     CDS             2..>357
                     /locus_tag="IU325_23515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811930.1"
                     /translation="MSNTKCDKLSFYEEKIRWRMRRQISNAEAIFYENQEEHIQRFLG
                     RYTCRKPVLVYWESEQRWTSVNGLEVMSMFDEYCHHIYLDDINKKIQAELPEDKTLGF
                     KRQLQFFILGDEKIRI"
BASE COUNT          125 a           54 c           75 g          103 t
ORIGIN      
        1 tttgagtaat accaaatgcg acaaattgtc tttttatgaa gaaaaaatcc gctggcgaat
       61 gcgtcggcaa atctcgaatg cagaggcaat attttacgag aatcaggaag aacacattca
      121 gcgcttttta ggcagatata cctgccgcaa accggttctt gtttattggg aaagtgaaca
      181 aagatggaca tctgtaaacg gtctggaagt aatgagtatg tttgatgagt attgtcatca
      241 catctacctt gatgatatca ataaaaaaat acaagctgaa ttacctgaag ataaaacatt
      301 aggtttcaaa cgtcaattgc aatttttcat tttaggggat gagaagatta gaatatg
//
LOCUS       DACBVI010000418          350 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.428, whole genome shotgun sequence.
ACCESSION   DACBVI010000418 DACBVI010000000
VERSION     DACBVI010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.428"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..>350
                     /locus_tag="IU325_23520"
     CDS             <1..>350
                     /locus_tag="IU325_23520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811931.1"
                     /translation="KSPHRMHIDSDIPTPSSEPINQFARQLITLLDTSDLSSMLSYCV
                     TQEFTANCRKISQNCYSTALFTINFATSPIHAENILITLHYKKEIISLLLETTPIKAN
                     HLRSILDYIEQEQL"
BASE COUNT          116 a           88 c           44 g          102 t
ORIGIN      
        1 ataagtctcc tcatagaatg cacatcgata gcgacatacc aacgccttca tcagagccta
       61 ttaatcaatt tgcgcgccaa ctcatcaccc tacttgatac ctctgactta agttcgatgc
      121 tgtcatactg tgttactcag gaatttaccg caaactgtcg aaaaatatca caaaattgtt
      181 attccactgc cctttttacc attaactttg ccacttcacc catccatgca gaaaatatac
      241 tcattacatt acactataaa aaagaaatca tttccttatt actggaaacc acgcctatta
      301 aagctaacca tttgcgaagc atactggatt atattgaaca ggaacagtta
//
LOCUS       DACBVI010000419          342 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.429, whole genome shotgun sequence.
ACCESSION   DACBVI010000419 DACBVI010000000
VERSION     DACBVI010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.429"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..204)
                     /locus_tag="IU325_23525"
     CDS             complement(<1..204)
                     /locus_tag="IU325_23525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811932.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDEL"
BASE COUNT          113 a           66 c           51 g          112 t
ORIGIN      
        1 caactcatca ttttttatga gatcactatc agcatttatt gctaatatca gatgcaagta
       61 gatggtgtcc catgtagaca actcaccctt acgactggaa tttaatatcg cgaaaaattc
      121 cctcagtgaa tcgaggaaat tatcactttg attcagttgt gtgcctggaa atgatgatgc
      181 ttgatcagat atattgttta tcatattaaa aatcacccaa gggaaaagat attccactga
      241 actcctcgtg ctttattatt ataaaagcac aacgcattta tactcatatt aattaacgaa
      301 acactgatac agcttgattt attcttcgct caaactcatt ag
//
LOCUS       DACBVI010000420          335 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.430, whole genome shotgun sequence.
ACCESSION   DACBVI010000420 DACBVI010000000
VERSION     DACBVI010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.430"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..>335
                     /locus_tag="IU325_23530"
     CDS             <1..>335
                     /locus_tag="IU325_23530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811933.1"
                     /translation="NRCIPDSLPAVEWLTYGSGWLSGMKLGDTPLVEYTRDRLHRETL
                     RSFGRYELTTAYTSAGQLQSQHLNSLQYDRDYTWNDNGELIRISSPRQTRSYSYSDTG
                     RLTGVHTTAA"
BASE COUNT           76 a          121 c           93 g           45 t
ORIGIN      
        1 aaccgctgta taccggacag cctgcccgcc gtggaatggc tgacctacgg cagcggctgg
       61 ctgtcaggca tgaaactcgg cgacacaccg ctggtggagt acacccgcga ccgcctgcac
      121 cgggaaacgc tgcgcagctt cggccgttat gaactcacca ccgcttacac ctctgccggg
      181 cagttacaga gccagcacct gaacagcctg cagtatgacc gcgattacac ctggaacgac
      241 aacggcgaac tcatccgcat cagcagcccg cgccagaccc ggagttacag ctacagcgac
      301 accggcaggc tgaccggcgt ccacaccacg gcagc
//
LOCUS       DACBVI010000421          335 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.431, whole genome shotgun sequence.
ACCESSION   DACBVI010000421 DACBVI010000000
VERSION     DACBVI010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.431"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..>335
                     /locus_tag="IU325_23535"
     CDS             <1..>335
                     /locus_tag="IU325_23535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP5811934.1"
                     /translation="TIFSNGEINNLFIKKSNIFGTSFTNTKIKNLRCEDIMPGRWTTQ
                     LVNKHLGYRYTGVFKTLASIDDKPSRFEILIPLIQTLVRDNVKLNNDVYKELNKFMHD
                     YDKTSPEMR"
BASE COUNT          129 a           62 c           53 g           91 t
ORIGIN      
        1 acaccatctt ttcaaatgga gaaataaata acctcttcat taaaaaaagc aacatatttg
       61 gaacatcgtt cactaatacc aagataaaaa atttgcgctg tgaagatatt atgcctggtc
      121 gatggaccac tcaacttgtc aataagcact taggttacag atatacagga gtattcaaaa
      181 ccctggcaag tattgatgac aaaccgtccc gtttcgaaat tttgatacca ttgatacaaa
      241 ctttagttcg cgacaatgta aagctaaata atgatgtgta taaagaactt aataagttta
      301 tgcacgatta cgacaaaaca tccccagaaa tgaga
//
LOCUS       DACBVI010000422          331 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.433, whole genome shotgun sequence.
ACCESSION   DACBVI010000422 DACBVI010000000
VERSION     DACBVI010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.433"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..281
                     /locus_tag="IU325_23540"
     CDS             <1..281
                     /locus_tag="IU325_23540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000948389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811935.1"
                     /translation="EFNTLKNRKEFLSSVINVNETRLQASAPAMPAIEALNAFIDKGD
                     YLKSLYVNKTRTAAEQKRLNEILRAQVISGLKASEGLAKLLIASGIML"
BASE COUNT          114 a           56 c           76 g           85 t
ORIGIN      
        1 cagagtttaa cactctgaaa aacaggaagg agtttctaag ttcagtaatc aatgtgaatg
       61 aaaccagact acaggcatcg gcacctgcca tgcctgcgat tgaggcttta aatgccttta
      121 tagataaggg tgattatcta aaaagcttat acgttaataa aacacgcacg gcagctgaac
      181 aaaaaagatt aaatgaaata ttacgcgcac aggtcattag tgggttaaag gcgtctgagg
      241 ggctggcaaa attacttatt gccagcggaa taatgttata ggaggctttt tatgaacgat
      301 gtgcaaaacc gtagaatact ggcattactt g
//
LOCUS       DACBVI010000423          330 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.434, whole genome shotgun sequence.
ACCESSION   DACBVI010000423 DACBVI010000000
VERSION     DACBVI010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.434"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..>330
                     /locus_tag="IU325_23545"
     CDS             <1..>330
                     /locus_tag="IU325_23545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP5811936.1"
                     /translation="RLNDRTILNSYDIVHSHGLRPDVINCKTSKNVINVSTLHSFILD
                     DLKNKYGIKGYLIAFAWLKLLKRFDSCIAISEATKRFYVKHGLKCHHIYNGIDFPDIE
                     LYQDNSRL"
BASE COUNT          114 a           47 c           56 g          113 t
ORIGIN      
        1 aggctaaatg atagaaccat tttaaacagt tatgatattg ttcactctca tggcttaaga
       61 cccgatgtta ttaattgtaa gaccagcaaa aatgtcatta atgtaagtac gcttcatagt
      121 tttattttag atgatcttaa gaataaatat ggaataaaag gatatctgat cgcttttgct
      181 tggcttaaat tattaaagcg atttgattca tgtattgcaa ttagtgaggc gactaaacgg
      241 ttttatgtta aacatggtct taaatgccac catatatata atggaatcga ctttcctgac
      301 atcgaattat atcaagataa ctcaaggcta
//
LOCUS       DACBVI010000424          329 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.435, whole genome shotgun sequence.
ACCESSION   DACBVI010000424 DACBVI010000000
VERSION     DACBVI010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.435"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(70..198)
                     /locus_tag="IU325_23550"
                     /pseudo
     CDS             complement(70..198)
                     /locus_tag="IU325_23550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950586.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT           95 a           68 c           59 g          107 t
ORIGIN      
        1 ataaggaata tctcaattat tgaatattta gtgcgaatta tttagtacaa aaaagcagcg
       61 ttgcgtgatc tttccctgcc ctttattcat aaacacatgg atatggtgat caacggtggt
      121 ctgatggagg cgcagagcct gagtaatcat gactgaattt cagccttcag aagccagaaa
      181 aatggcttta atacaatcgc agccccgctt ttcacaacat tatgcttata atatgaaaaa
      241 tatcttgcct tgcatctttg tgcctacggc attggatgat ctttccctaa ccttatagtt
      301 tcttttagcc tttcaagcct tagcaattc
//
LOCUS       DACBVI010000425          328 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.436, whole genome shotgun sequence.
ACCESSION   DACBVI010000425 DACBVI010000000
VERSION     DACBVI010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.436"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(<1..>328)
                     /locus_tag="IU325_23555"
     CDS             complement(<1..>328)
                     /locus_tag="IU325_23555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat family protein"
                     /protein_id="HAP5811937.1"
                     /translation="VLTGLVDRFGRTQTFHREAGGEFSGEITGVTDGAGRHFRLVLTT
                     QAQRAEEARQQASSGGTEPSAFPDTLPDYTEYGRDNGIRLSAVWLTHDPEYPENLPAA
                     PLVRYGW"
BASE COUNT           57 a          112 c          101 g           58 t
ORIGIN      
        1 tccagccata gcgcaccagc ggcgcggcag gtaaattctc cgggtattcc gggtcgtgtg
       61 tcagccacac ggcagacaga cggataccgt tgtcccggcc atattccgtg tagtccggca
      121 gggtatcagg aaaagcggac ggttccgtcc cgccggaaga ggcctgctgc cgggcctctt
      181 ctgcccgctg cgcctgcgtg gtcagtacca gccggaagtg acgcccggca ccatccgtca
      241 cgccggtgat ttcgccgctg aattcaccgc cagcctcacg gtgaaacgtc tgtgtgcgcc
      301 cgaagcggtc caccagcccg gtcagtac
//
LOCUS       DACBVI010000426          319 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.437, whole genome shotgun sequence.
ACCESSION   DACBVI010000426 DACBVI010000000
VERSION     DACBVI010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.437"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            63..305
                     /locus_tag="IU325_23560"
     CDS             63..305
                     /locus_tag="IU325_23560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP5811938.1"
                     /translation="MIDEFHVMYMYKKIQVEAATTDIKTLEQFLKKFRQVVAERREEY
                     YREIGEKKARKLRMKKAQRLLEKMTCDRVSPEDLKE"
BASE COUNT          116 a           50 c           75 g           78 t
ORIGIN      
        1 gtatgatgaa cattccctcg tgcagaggga aataatcctg taaagaatcg gagataacca
       61 atatgattga tgaattccat gttatgtata tgtacaaaaa aatccaagtt gaagcggcta
      121 ctaccgacat caaaacactg gaacagtttt tgaagaagtt ccgccaagtt gtcgcggagc
      181 gccgggaaga atactatcgg gagattggtg agaaaaaagc acgtaagttg agaatgaaaa
      241 aagcacaaag attacttgag aaaatgacat gtgatcgtgt ttcaccagag gatctcaagg
      301 aatgactatt tgagtttat
//
LOCUS       DACBVI010000427          313 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.438, whole genome shotgun sequence.
ACCESSION   DACBVI010000427 DACBVI010000000
VERSION     DACBVI010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.438"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..>313
                     /locus_tag="IU325_23565"
     CDS             <1..>313
                     /locus_tag="IU325_23565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP5811939.1"
                     /translation="RRFAGACRFVFNRALALQNENHEAGNKYIPYGKMASWLVEWKNA
                     TETQWLKDAPSQPLQQSLKDLERAYKNFFQKRAAFPRFKKRGQNDAFRYPQGVKLDQE
                     N"
BASE COUNT           85 a           77 c           81 g           70 t
ORIGIN      
        1 tgaggcgctt cgcaggcgca tgtcgtttcg ttttcaatcg tgctctggcg cttcagaatg
       61 agaatcatga ggccggtaat aaatacatcc cttacgggaa aatggcttcc tggctggttg
      121 agtggaaaaa tgccactgaa acgcaatggc ttaaagatgc cccgtcacag ccattgcaac
      181 agtcactgaa agaccttgag cgggcttaca aaaacttctt ccagaagcgg gctgcttttc
      241 cccgattcaa aaagcgggga cagaatgatg cattccgcta cccgcagggc gttaagctcg
      301 atcaggaaaa cag
//
LOCUS       DACBVI010000428          305 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.439, whole genome shotgun sequence.
ACCESSION   DACBVI010000428 DACBVI010000000
VERSION     DACBVI010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.439"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..>305
                     /locus_tag="IU325_23570"
     CDS             <1..>305
                     /locus_tag="IU325_23570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HAP5811940.1"
                     /translation="KGDLFKKRTLWEFVRDLFPGSHIKEVKGLIYEFVTKVDNKAEVF
                     DKIKSLAKKEQQWRFSTKTDFTTNENNEVIVSRSFNLYTGATSNDNEKKQVSSERLT"
BASE COUNT          111 a           49 c           61 g           84 t
ORIGIN      
        1 caaaggggat ttatttaaaa aacgtacact gtgggagttt gttcgcgatc tttttcccgg
       61 ctctcatatt aaagaagtca aaggcttaat ttacgaattt gtcacgaaag ttgataacaa
      121 agccgaggtg ttcgataaga ttaaatctct ggcaaaaaaa gaacaacaat ggcgatttag
      181 tactaagaca gactttacca caaatgaaaa taatgaagtt attgtgagta gatcctttaa
      241 tctctacacg ggggctactt cgaatgataa cgaaaaaaaa caggtttctt cagagagatt
      301 aacgc
//
LOCUS       DACBVI010000429          303 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.440, whole genome shotgun sequence.
ACCESSION   DACBVI010000429 DACBVI010000000
VERSION     DACBVI010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.440"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            complement(49..222)
                     /locus_tag="IU325_23575"
     CDS             complement(49..222)
                     /locus_tag="IU325_23575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811941.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
BASE COUNT           97 a           68 c           72 g           66 t
ORIGIN      
        1 actgacgcgc cgggcatcct caaataggaa aacctgaagg cacgattgtt aaatctccca
       61 cggtgccttc gggatgtctg gcgtagattc agcaacctgg cgcaagagtt ctgctttaag
      121 tatctcgaga ttatggatcg cagagtggta atcgcctgcc acaaggatat ctaacacaat
      181 atcaatacgt tcggcaacgc gctgggcatc aaattcagac atcaaggaca tccttatagc
      241 aaaagtggaa aaggaataat gcaaaaattg ccgctaaaag agaagtgttt aacagcaacg
      301 gct
//
LOCUS       DACBVI010000430          302 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.441, whole genome shotgun sequence.
ACCESSION   DACBVI010000430 DACBVI010000000
VERSION     DACBVI010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.441"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..259
                     /locus_tag="IU325_23580"
     CDS             <1..259
                     /locus_tag="IU325_23580"
                     /inference="COORDINATES: protein motif:HMM:NF012360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811942.1"
                     /translation="NCIKGIFIGDHVWIGKKALIQKGTVIPEDNIIGFGAMVNKPLNG
                     SNKVFAGVPVKEIKSGVTWSRSECEEIAISSLNDWRNLPYV"
BASE COUNT           99 a           40 c           66 g           97 t
ORIGIN      
        1 aaattgtata aaggggattt ttattggtga tcatgtatgg ataggtaaaa aagcactgat
       61 tcaaaaaggt accgtaattc cagaagataa tataattggg tttggtgcaa tggtaaacaa
      121 accattgaat ggttccaata aggtttttgc tggtgttcct gttaaagaga ttaaatctgg
      181 tgtaacttgg tcacgaagtg aatgcgaaga aattgctata tcatctctta acgactggcg
      241 taaccttcca tatgtttgag ataacacctt tcctcagtag gtatgaatct aataggaatt
      301 tg
//
LOCUS       DACBVI010000431          302 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.442, whole genome shotgun sequence.
ACCESSION   DACBVI010000431 DACBVI010000000
VERSION     DACBVI010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.442"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
BASE COUNT          108 a           55 c           51 g           88 t
ORIGIN      
        1 tgatgaaact gtcttattct taaattttag ataacaggta taaatgattc atactttcag
       61 ggagttggca tctaaccaag atgggtacag actaaattag tgtatcgtta cacgcagtta
      121 atagagatac cgaccactat ttaaaatcgt tggtggagtt gattttagcg tccacacgtg
      181 tcacatgtaa gcttaataca taattatgca ataattgcac gaaatagctg aaactctcac
      241 tccaaaatat gaaaacaatg ttcctaaaaa cgtcctcagt catacgaacc aaggactatc
      301 aa
//
LOCUS       DACBVI010000432          302 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.443, whole genome shotgun sequence.
ACCESSION   DACBVI010000432 DACBVI010000000
VERSION     DACBVI010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.443"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            118..>302
                     /locus_tag="IU325_23585"
     CDS             118..>302
                     /locus_tag="IU325_23585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP5811943.1"
                     /translation="MESANLDLEENKEIASKFERALGMGATLAELHGITPDTLEGVYA
                     YAYNFYEKGRLDEAELF"
BASE COUNT          107 a           49 c           73 g           73 t
ORIGIN      
        1 tttaatgcag aaaaggaagc ccaacggatg ggcataaaaa tgagcgaatt ttgatatcac
       61 ccccctctaa aaaatggtga agtctgaaca aagaatgttc gaaaggagag agtagagatg
      121 gaaagcgcaa atctggattt agaagaaaat aaagaaattg ccagtaagtt cgaacgtgca
      181 ttaggtatgg gagcaacact tgcagagctg catggcatta ctccggatac ccttgaaggc
      241 gtttatgctt acgcttataa cttttatgaa aaaggaagac ttgacgaagc tgagctcttt
      301 tt
//
LOCUS       DACBVI010000433          300 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.denovo.445, whole genome shotgun sequence.
ACCESSION   DACBVI010000433 DACBVI010000000
VERSION     DACBVI010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.denovo.445"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..112
                     /locus_tag="IU325_23590"
     CDS             <1..112
                     /locus_tag="IU325_23590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP5811944.1"
                     /translation="NCYLDKYGDANINDYYRDLEIKHSQPQNRASGKRVS"
BASE COUNT           95 a           58 c           60 g           87 t
ORIGIN      
        1 aaattgttat ttagataaat atggcgatgc aaatatcaat gattattata gagacttaga
       61 aataaaacac tcacaacccc aaaacagagc atcaggcaaa agagtaagtt aacatcattg
      121 ttaattaatt cgaccagtca gcaaatatgc tgactggtcg aacatagttt gctaaaaagt
      181 catcactggc tgatgtcagt cacaccaccc gccagttcaa tctgcgggta tttccagcgt
      241 atcgcggcac gcgattgctg gtctgttgat gtagtggatt gttgttgctg acttttctta
//
LOCUS       DACBVI010000434        12038 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.guided.132, whole genome shotgun sequence.
ACCESSION   DACBVI010000434 DACBVI010000000
VERSION     DACBVI010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.guided.132"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..2959
                     /gene="mscM"
                     /locus_tag="IU325_23595"
     CDS             <1..2959
                     /gene="mscM"
                     /locus_tag="IU325_23595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HAP5811945.1"
                     /translation="QAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLGTLTGNS
                     PLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQLDAYLQA
                     LRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQAQRMDLV
                     ASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQQLDTEM
                     AQLRVQRLRYEDLLNKQPQLRQIHQADGQPLTAEQNRILEAQLRTQRELLNSLLQGGD
                     TLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDLRRLISL
                     DTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVGKVTQDH
                     FWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLLWVVMIC
                     ATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDREFSGSLG
                     RLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILASAV
                     GYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQRA
                     RGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIHSA
                     FGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVLIITTQLVRNLPALLELAILQH
                     LDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEIFA
                     NFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFITEQ
                     FINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQQG
                     IQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTGRT
                     LTSAGKGRQAGSL"
     gene            complement(2988..3302)
                     /locus_tag="IU325_23600"
     CDS             complement(2988..3302)
                     /locus_tag="IU325_23600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HAP5811946.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEDDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(3400..4902)
                     /gene="yjeM"
                     /locus_tag="IU325_23605"
     CDS             complement(3400..4902)
                     /gene="yjeM"
                     /locus_tag="IU325_23605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HAP5811947.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRITGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWVVE"
     gene            complement(5121..6098)
                     /gene="epmA"
                     /locus_tag="IU325_23610"
     CDS             complement(5121..6098)
                     /gene="epmA"
                     /locus_tag="IU325_23610"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAP5811948.1"
                     /translation="MSETASWQPSASIPNLLKRATIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            6423..8231
                     /gene="frdA"
                     /locus_tag="IU325_23615"
     CDS             6423..8231
                     /gene="frdA"
                     /locus_tag="IU325_23615"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HAP5811949.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            8224..8958
                     /locus_tag="IU325_23620"
     CDS             8224..8958
                     /locus_tag="IU325_23620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAP5811950.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            8969..9364
                     /gene="frdC"
                     /locus_tag="IU325_23625"
     CDS             8969..9364
                     /gene="frdC"
                     /locus_tag="IU325_23625"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAP5811951.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPESWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            9375..9734
                     /gene="frdD"
                     /locus_tag="IU325_23630"
     CDS             9375..9734
                     /gene="frdD"
                     /locus_tag="IU325_23630"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAP5811952.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            9797..10930
                     /gene="blaEC"
                     /locus_tag="IU325_23635"
     CDS             9797..10930
                     /gene="blaEC"
                     /locus_tag="IU325_23635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAP5811953.1"
                     /translation="MFKMTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLTHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDITEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKAITPPAPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            11018..11551
                     /gene="blc"
                     /locus_tag="IU325_23640"
     CDS             11018..11551
                     /gene="blc"
                     /locus_tag="IU325_23640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Blc"
                     /protein_id="HAP5811954.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDTKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGDPRRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAIAT
                     REGFDISKFIWVQQPGS"
     gene            complement(11548..11865)
                     /locus_tag="IU325_23645"
     CDS             complement(11548..11865)
                     /locus_tag="IU325_23645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HAP5811955.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
BASE COUNT         3017 a         3180 c         3134 g         2707 t
ORIGIN      
        1 tcaggcccag caagagcagg aacgcgcccg cgagattgcc gattcgctga atcaactgcc
       61 gcaacagcaa accgatgccc gccgtcagtt aaatgaaatc gagcgccgcc tggggacgct
      121 taccggcaat tccccgctca atcaggcaca aaatttcgcg ttgcagtctg actccgcacg
      181 cctgaaagcg ctcgtcgatg aactggaact ggcgcagctg tccgccaata accgccagga
      241 attagcgcgc ttacgctcag agctggcgga aaaagagagc cagcaactgg atgcgtattt
      301 gcaggcctta cgtaatcagt tgaacagcca acgtcagctt gaggcggagc gggcgctgga
      361 aagtaccgaa ttgctggcag aaaacagcgc cgatttgccg aaagacatcg tcgcgcaatt
      421 caaaattaac cgcgaactat cagcggcgct gaatcaacag gcgcagcgga tggatctcgt
      481 tgcctcgcaa cagcgtcagg ctgccagcca gacgttacag gtccggcaag cgttgaatac
      541 gctgcgtgaa cagtcgcaat ggctgggatc gtccaatctg ctcggcgaag cgttgcgggc
      601 gcaggtggca cggctgccgg aaatgccaaa accacaacag cttgataccg aaatggcgca
      661 gttgcgtgtg caacggttac gttatgagga tctgcttaat aaacagccgc agctacggca
      721 aatccatcag gccgacggtc agccgctgac cgccgagcaa aaccgtattc tggaagcaca
      781 gctacgcact cagcgtgagt tgctgaactc attgttgcag ggtggagaca cgctactact
      841 ggaactgacc aagctgaaag tctccaacgg gcaactggag gatgcgctga aagaggtgaa
      901 tgaagcgacg caccgctatc tgttctggac ctctgacgtg cgcccgatga ccatcgcctg
      961 gccgctggaa atcgcccagg atctgcgtcg tctcatttcg ctggacacct tcagtcagtt
     1021 gggcaaagcc agtgtgatga tgctgaccag taaagagaca attttaccgc tgtttggcgc
     1081 gttgattctg gtcggttgca gtatttactc acgccgctat ttcacccgtt ttcttgaacg
     1141 ttcggcggcg aaagtcggca aagtgactca ggatcacttc tggctgacgt tacgcactct
     1201 tttctggtcg attctcgtcg cgtcaccgct accggtgctg tggatgacgc tgggttacgg
     1261 cttgcgcgag gcgtggcctt atccgctggc ggtcgcgatt ggtgatggcg taacggccac
     1321 cgtgccgctg ctgtgggtag tgatgatttg cgccaccttt gcccgcccga acggcttgtt
     1381 tatcgctcat tttggctggc cgcgcgaacg ggtttcccgt gggatgcgct actacctgat
     1441 gagcatcggg cttattgtgc cgctgattat ggcgctgatg atgttcgata acctcgacga
     1501 ccgtgaattc tccggttcgc tgggacggct ttgctttatc ctcatttgcg gtgcgctggc
     1561 ggtggtcact ctcagtctga aaaaggccgg gatcccgctg tatctcaaca aagaaggcag
     1621 cggtgacaac attaccaacc atatgctgtg gaacatgatg attggcgcac cgctggttgc
     1681 cattctggcg tcggcggtgg gttatctggc aacggcgcag gcgctgttag cgaggcttga
     1741 aacctcggtt gccatctggt ttttgctact ggtggtttat cacgttatcc gccgctggat
     1801 gctgatccaa cgccgcaggc tggcgtttga ccgggcgaag catcgccggg cagagatgtt
     1861 agcgcaacgt gcgcgtggcg aagaggaagc acatcatcac agtagcccgg aaggggcaat
     1921 tgaagtcgat gaaagcgaag tcgatctcga tgccatcagt gcgcagtcct tgcggctggt
     1981 gcgctcaatt ttgatgttga tcgccctgct ttcggtcatt gtgctgtggt cagaaatcca
     2041 ttccgctttc ggcttcctcg aaaatatttc gctgtgggat gtcacctcca cggtacaggg
     2101 cgtagaaagt ctggagccga ttaccctcgg tgcggtgctg attgccattc tggtgcttat
     2161 catcaccacg cagctggtgc gcaacctgcc cgcgctgctg gaactggcga ttttgcagca
     2221 cctggattta acgccgggta caggttacgc catcaccacc atcaccaaat atctgctgat
     2281 gctgattggc gggctggtcg gcttctcaat gattggtatt gagtggtcga aattgcagtg
     2341 gctggtcgcc gcgctcggtg ttggtctcgg ttttggtttg caggaaattt tcgccaactt
     2401 tatctctggc ctgatcatcc tgttcgaaaa accgattcgc attggcgata cggtaacgat
     2461 ccgcgatctt actggtagcg tgacgaaaat taacacccgc gccaccacca tcagcgactg
     2521 ggaccgtaaa gagataatcg tgccgaacaa ggcgtttatt accgagcagt ttatcaactg
     2581 gtcgctctct gactcggtca cgcgcgtggt gttgacgatt ccggcccctg ccgatgccaa
     2641 cagtgaagaa gtgacggaaa tcctgctcac cgcagcgcgt cgctgctcgc tggtgatcga
     2701 caacccggca ccggaagtct tcctggtgga tctgcaacag gggattcaga ttttcgagct
     2761 gcgtatttac gccgctgaga tgggtcaccg tatgccgcta cgccatgaga tccaccagct
     2821 aattctggca ggcttccatg cccacggtat cgatatgcca ttcccgccct tccagatgcg
     2881 tctggaaagc ctgaatggta aacaaacggg gagaacatta acttctgcgg gcaaaggtcg
     2941 tcaggcggga agtttgtaag gttttgtgcc ggatgaaccc atccggctca caaacaaaac
     3001 tgatagcgca gaaaaaactg ccacagccag tagccgaaaa tagccagcgc ggtggcgtac
     3061 ataaaccagc cgcgtttttt tcgccacaaa gcgaaccaga gaaaagggaa aaagagcggt
     3121 aacgtcatta tcacaccgac atcgcgagta tcgtcatctt cgagatacat caaaacatca
     3181 cagacgttgt taatcccgcg tccatcaatc atccactctt tgtccagcgc ggaggtaatg
     3241 gttatccaga ggaacgccac aatgaaccag atgcagcaca agacaaacat gcgcgcactc
     3301 atgagataac ccgtagttta atttcgctat agcacatacg aaatcccact aattccctgt
     3361 cattgcgccc tatcgtcaga cagacgatag ggcatcgggt tattccacca cccactgcgg
     3421 attttttgcc actctgctgc aatagttctg gtaaatcgcc atcgctaaca gcgagaagaa
     3481 gacagggccg ccaatcatcc acaatgtgct gtcccagtct ccggcttcaa ccacaggttg
     3541 aatgatggtg aagacgttcg caaatgtcac caccagaacg acaaccaccg tcgcaatcat
     3601 tgccgacata cgcgttttga aaatcacaaa tggtctgtcg agatcctgac gtgctttaaa
     3661 gaatgggaaa gccagcgcga ggaacaggta aggaagcgtc atagacacgt tcgccatcag
     3721 cgtcagcttg ttaaagaacg ccgatgcggt accgccacca aacgaaacca gcaggatgaa
     3781 gacagtaacc aacccgcact gcatccacat ggcgatagac ggcatcccca tcgcattcag
     3841 gcgcgtcatc ggttccggcc acaacgcttt cggcgtcccc tggatgatgg ctttcagcgg
     3901 tgaatagcaa agcgtaaaga acgcaccggt ataagcgagg aacatcgaaa gtccggtaat
     3961 acgcgcaaac catacgccca gcgacaatga cgcttctggt gacaaatgca gtgcgttacc
     4021 cagcgtcacc ccgaggctct tcatcagcac ataggtaata ttgccgaggt taacggaacc
     4081 attacttaat acctgctgcc agtttgtgct gacgccccat aaaaatattg ccagcgaata
     4141 accgattgaa ataacaatag cggcaaaaac aatacctttg gcaaagttct tttctggatt
     4201 ttccgtttta tcgaccagac caccaaccgc ttcaattccg ccataggcaa aaatagcaaa
     4261 taccacaaac gatagcattg ccagaccgga ctgataacct gggttcggtg atgcaaggaa
     4321 attaatatcc tgcgcgaaat gcccaccatt taataacaaa atagtaatgc ttactaacag
     4381 caatactaaa ttcagacaca ttactgcaat accgcccacc gcagtaatgc gggcaatttt
     4441 attaatcccc tttgaagcaa cgacggtgac cagaatcatc catgccactg ccagcagacc
     4501 aaccacctgc gtaggctcca gtccggtaat acgccagtgc tgggtcatgt cgctaccata
     4561 gaggaatgtt gagaacggaa cccaaacttt cgcggaggta ctcaccatcc agatgatata
     4621 agaggaaaac cacataaatg taccaatgaa ggcaaaacgt ggtccgacac tattattcat
     4681 ccaggaatag ataccacctt cttctttgcg ataagcagct cccatttcag ccatcattaa
     4741 ggcgaatgga ataaagaata acaatgcaga aaatatataa aagggaatcg cactataacc
     4801 cattaagtaa taagccgatg ggctattggc aaatccaaat acggaagtaa agatcatcaa
     4861 tatgagtcct atcagactca tcttttttat cgtgtgaggc atgaaaccat ccttcatttc
     4921 agcgcgtgta taaaacgcgc ccactgaact gcactgccca acattgagcg ggcagcgtaa
     4981 aaaccaatac agaattgata cctggatgat atcaaatatc cagtcttaaa ttgtggcctt
     5041 tagcggaaaa atgaaaacat atgctacaga atggcgctta tcacgccatt cttcgcggtt
     5101 aattcagtaa tttttcagaa ttatgcccgg tcaacgctaa aggcgatgac ttcagccagt
     5161 gtctccgcgc ccagcgccaa catcaccaga cgatcaacgc ctaatgccac gccggaacag
     5221 tcaggcatac cgactttcaa ggcttcaatc agattctggt caatggggtg ctgcggcaaa
     5281 ccgcgcgccg cgcgcttacg gttatcttgt tcaaagcgtt gttgctgctc acgggcatcc
     5341 gtcaattcat ggaaaccatt cgccagctca atacctttat aataaacctc aaagcgttca
     5401 gcgacccgat gatcttcggt actgatttgc gccagtgatg cctggctggc cggaaagtgg
     5461 tacacaaagg tcggtttttc tttgccaata tttggctcta cgccaaaggt aaacagcagt
     5521 tgtagcagcg tgtcgcggtc ttcttcggta tcggcaacgt tgctcaaatc cagtttcgct
     5581 gccacttccc gcagttgcgt tttgtcagca gagagtgggt caatttccag ataacgcaag
     5641 aaagcttgtt gataagaaag gctttctgct gccgggcagt ccagcacctg ttgtaagaga
     5701 tcgtccacct cgttcatcaa ccggtacata tcatagtgcg gtcgatacca ctcaagcata
     5761 gtgaactcag ggttgtgata acgccccatc tcttcattac ggaagctgcg gcacagctgg
     5821 aataccggcc cacaaccagc aaccagcagg cgtttcatat ggtattccgg gctggtcatt
     5881 aaccagagat tcatcccctg cgaatgcccg gggcctacga aacgtgtctc aaacgggacc
     5941 aaatgaatat cggttaccgt cgcctggctc atacaaggcg tctccacctc cagcactcca
     6001 cgatcggcaa agaaacgacg gatctccgcc ataatcgtcg cgcgttttaa taagttagga
     6061 atggatgcgc tcggctgcca ggatgccgtt tcgctcatag ttaaatctcc agtttttgac
     6121 aagggcacga agtctactcg caacgcgacg gcgagacaaa ttttacgcag gaatcaaaca
     6181 agggtgggca gtgactaaaa aaagcacgat ctgatggttt agtaattaaa ttaatcatct
     6241 tcagtgataa tttagccctc ttgcgcacta aaaaaatcga tctcgtcaaa tttcagactt
     6301 atccatcaga ctatactgtt gtacctataa aggagcagtg gaatagcgtt cgcagaccgt
     6361 aactttcagg cacttaccct gaagtacgtg gctgtgggat aaaaacaatc tggaggaatg
     6421 tcgtgcaaac ctttcaagcc gatcttgcca ttgtaggcgc cggtggcgcg ggattacgtg
     6481 ctgcaattgc tgccgcgcag gcaaatccaa atgcaaaaat cgcactaatc tcaaaagtat
     6541 acccgatgcg tagccatacc gttgctgcag aagggggctc cgccgctgtc gcgcaggatc
     6601 atgacagctt cgaatatcac tttcacgata cagtagcggg tggcgactgg ttgtgtgagc
     6661 aggatgtcgt ggattatttc gtccaccact gcccaaccga aatgacccaa ctggaactgt
     6721 gggggtgccc atggagccgt cgcccggatg gtagcgtcaa cgtacgtcgc ttcggcggca
     6781 tgaaaatcga gcgtacctgg ttcgccgccg ataagaccgg cttccatatg ctgcacacgc
     6841 tgttccagac ctctctgcaa ttcccgcaga tccagcgttt tgacgaacat ttcgtgctgg
     6901 atattctggt tgatgatggt catgttcgcg gcctggtagc aatgaacatg atggaaggca
     6961 cgctggtgca gatccgtgct aacgcggtcg ttatggctac cggcggtgcg ggtcgcgttt
     7021 atcgttacaa caccaacggc ggcatcgtta ccggtgacgg tatgggtatg gcgctaagcc
     7081 acggcgttcc gctgcgtgac atggaattcg ttcagtatca cccaaccggt ctgccaggtt
     7141 ccggtatcct gatgaccgaa ggctgccgcg gtgaaggcgg tattctggtc aacaaaaatg
     7201 gctaccgtta tctgcaagat tacggcatgg gcccggaaac tccgctgggc gagccgaaaa
     7261 acaaatatat ggaactgggt ccacgcgaca aagtttctca ggccttctgg cacgaatggc
     7321 gtaaaggcaa caccatctcc acgcctcgtg gcgatgtggt ttatctcgac ctgcgtcacc
     7381 tcggcgagaa aaaactgcat gaacgtctgc cgttcatctg cgaactggcg aaagcgtacg
     7441 ttggcgtcga tccggttaaa gaaccgattc cggtacgtcc gaccgcacac tacaccatgg
     7501 gcggtatcga aaccgatcag aactgtgaaa cccgcattaa aggtctgttc gccgtgggtg
     7561 aatgttcctc tgttggtctg cacggtgcaa accgtctggg ctccaactcc ctggcggaac
     7621 tggtggtctt cggccgtctg gccggtgaac aagcgacaga gcgtgcagca actgccggta
     7681 atggcaacga agcggcaatt gaagcgcagg cagctggcgt tgaacaacgt ctgaaagatc
     7741 tggttaacca ggatggcggc gaaaactggg cgaagatccg cgacgaaatg ggcctggcta
     7801 tggaagaagg ttgcggtatc taccgtacgc cggaactgat gcagaaaacc atcgacaagc
     7861 tggctgagct gcaggaacgc ttcaagcgcg tgcgcatcac cgacacctcc agcgtgttca
     7921 acaccgacct gctctacacc attgaactgg gccacggtct gaacgttgct gaatgtatgg
     7981 cgcactccgc aatggcacgt aaagagtccc gcggcgcaca ccagcgtctg gacgaaggtt
     8041 gcaccgagcg tgacgacgtc aacttcctca aacacaccct cgccttccgc gatgctgatg
     8101 gcacgactcg cctggagtac agcgacgtga agattactac gctgccgcca gctaaacgtg
     8161 tttacggtgg cgaagcggat gcagccgata aggcggaagc agccaataag aaggagaagg
     8221 cgaatggctg agatgaaaaa cctgaaaatt gaggtggtgc gctataaccc ggaagtcgat
     8281 accgcaccgc atagcgcatt ctatgaagtg ccttatgacg caactacctc attactggat
     8341 gcgctgggct acatcaaaga caacctggca ccggacctga gctaccgctg gtcctgccgt
     8401 atggcgattt gtggttcctg cggcatgatg gttaacaacg tgccaaaact ggcatgtaaa
     8461 accttcctgc gtgattacac cgacggtatg aaggttgaag cgttagctaa cttcccgatt
     8521 gaacgcgatc tggtggtcga tatgacccac ttcatcgaaa gtctggaagc gatcaaaccg
     8581 tacatcatcg gcaactcccg caccgcggat cagggtacta acatccagac cccggcgcag
     8641 atggccaagt atcaccagtt ttccggttgc atcaactgtg gtctgtgcta cgccgcgtgc
     8701 ccgcagtttg gcctgaaccc agagttcatc ggtccggctg ccattacgct ggcgcatcgt
     8761 tacaacgaag atagccgcga ccacggtaag aaggagcgta tggcgcagtt gaacagccag
     8821 aacggcgtat ggagctgtac tttcgtgggc tactgctccg aagtctgccc gaaacacgtc
     8881 gatccggctg cggccattca gcagggcaaa gtagaaagtt cgaaagattt tcttatcgcg
     8941 accctgaaac cacgctaagg agtgcaacat gacgactaaa cgtaaaccgt atgtacggcc
     9001 aatgacgtcc acctggtgga aaaaattgcc gttttatcgc ttttacatgc tgcgcgaagg
     9061 cacggcggtt ccggctgtgt ggttcagcat tgaactgatt ttcgggctgt ttgccctgaa
     9121 aaatggcccg gaaagctggg cgggattcgt cgacttttta caaaacccgg ttattgtgat
     9181 cattaacctg atcactctgg cggcagctct gctgcacacc aaaacctggt ttgaactggc
     9241 accgaaagcg gccaatatca ttgtaaaaga cgaaaaaatg ggaccagagc caattatcaa
     9301 aagtctctgg gcggtaactg tggttgccac catcgtaatc ctgtttgttg ccctgtactg
     9361 gtaaggagcc tgagatgatt aatccaaatc caaagcgttc tgacgaaccg gtattctggg
     9421 gcctcttcgg ggccggtggt atgtggagcg caattattgc gccggtgatg atcctgctgg
     9481 tgggtattct gctgccgctg gggctgtttc cgggtgatgc actgagctac gagcgcgttc
     9541 tggcgttcgc gcagagcttc attggtcgcg tattcctgtt cctgatgatc gttctgccgc
     9601 tgtggtgtgg tttacaccgt atgcaccacg cgatgcacga tctgaaaatc cacgtacctg
     9661 cgggcaaatg ggttttctac ggtctggctg ctatcctgac agttgtcacg ctgattggtg
     9721 tcgttacaat ctaacgcatc gccaatgtaa atccggcccg cctatggcgg gccgttttgt
     9781 atggaaacca gaccctatgt tcaaaatgac gctctgcgcc ttattaatta ccgcctcttg
     9841 ctccacattt gctgcccctc aacaaatcaa cgatattgtg catcgcacaa taaccccgct
     9901 tatagagcaa caaaagatcc ccggtatggc ggtggcggta atttatcagg gtaaacctta
     9961 ttactttacc tggggctatg cggacatcgc caaaaagcag cccgtcacac agcaaacgtt
    10021 gtttgagtta ggctcggtca gcaaaacatt tacgggcgta cttggtggcg acgctattgc
    10081 acgaggggaa atcaagttaa gcgatcccgc gacaaaatac tggcctgaac ttaccgctaa
    10141 acaatggaat gggatcacac tattacatct cgcaacctac accgctggcg gcctgccatt
    10201 gcaggtgccg gatgaggtga aatcctcaag cgacttgctg cgcttctatc aaaactggca
    10261 acctgcatgg gctccaggaa cacaacgtct gtatgccaac tccagtatcg gtttgttcgg
    10321 cgcactggct gtgaagccgt ctggtttgag ttttgagcag gcgatgcaaa ctcgtgtctt
    10381 ccagccactc aaactcactc atacgtggat taatgtgccg tccgcagaag aaaagaatta
    10441 cgcctgggga tatcgcgaag gtaaggcagt gcatgtttcg ccaggggcgt tagatgctga
    10501 agcttatggt gtgaagtcga ccattgaaga tatggcccgc tgggtgcaaa gcaatttaaa
    10561 accccttgat atcactgaga aaacgcttca acaagggata caactggcac aatctcgcta
    10621 ctggcaaacc ggcgatatgt atcagggcct gggctgggaa atgctggact ggccggtaaa
    10681 tcctgacatc atcattaacg gcagtgacaa taaaattgca ctggcagcac gccccgtaaa
    10741 agcgattacg cccccagctc ctgcagtgcg cgcatcatgg gtacataaaa cgggggcgac
    10801 cggcggattt ggtagctatg ttgcgtttat tccagaaaaa gagctgggta tcgtgatgct
    10861 ggctaacaaa aactatccca atccagcgag agtcgccgcc gcctggcaga ttctcaacgc
    10921 tctacagtaa aatttcatcg ggtccgaatt ttcggacctt ttctgcgctt ttccttgctg
    10981 tcatctacac ttagaaaaaa ccagtaagga aacatttatg cgcctgctcc ctctcgttgc
    11041 cgcagcgaca gctgcatttc tggtggttgc ctgtagttct cccacaccac cgcgtggcgt
    11101 gaccgtggta aataatttcg ataccaaacg ctatctgggt acatggtatg agattgcccg
    11161 ttttgatcac cgttttgaac gcgggctgga gaaagtgacc gcgacgtata gcctgcggga
    11221 tgacggcggc ctgaatgtca ttaataaagg ctataacccg gacagagaga tgtggcagca
    11281 gagtgaagga aaagcctact ttactggaga tccgcgtcgc gcagccctga aagtgtcgtt
    11341 ctttgggcca ttctacggcg gttataatgt catcgcgctc gaccgggagt accgccacgc
    11401 gctggtttgc ggcccggacc gcgactacct gtggatactc tctcgcacgc caaccatttc
    11461 tgacgaagtg aaacaggaga tgctggcaat cgcgacccgg gaagggtttg atatcagtaa
    11521 gtttatttgg gtacagcaac ccggtagtta atgagtgctg agtttcagac caataatccc
    11581 caatacgatt aacgccagac tcgccaggcg catcgggtta gcggactcgc cgagcagcac
    11641 aatgccggtg atggccgcgc cgactgcgcc aatacccgtc cacacggcat aagccgtccc
    11701 tactggtaac gatttcatcg cccaggcaag tagcgccata ctgacaatca tcgccgtcac
    11761 agtaataaca cttggcgtca aacgactaaa gccgtgggta tatttcaggc caacggccca
    11821 taccacttcc agcagaccag caataactaa gataatccag gacatatcag gctccggaga
    11881 attggggccg tccccggtga aaagaggcgt ttgcaggtcg tcctgcaaag ctgatggttg
    11941 aacggatatt ttgccaggtg gcaggggaat ttcaactatt acctttgtga ctatcgcaaa
    12001 aaaacaaacg gcacgacata actgtcgtac cgttattg
//
LOCUS       DACBVI010000435         6586 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.guided.209, whole genome shotgun sequence.
ACCESSION   DACBVI010000435 DACBVI010000000
VERSION     DACBVI010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.guided.209"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            129..926
                     /locus_tag="IU325_23650"
     CDS             129..926
                     /locus_tag="IU325_23650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-181"
                     /protein_id="HAP5811956.1"
                     /translation="MRVLALSAVFLVASIIGMPAVAKEWQENKSWNAHFTEHKSQGVV
                     VLWNENKQQGFTNNLKRANQAFLPASTFKIPNSLIALDLGVVKDEHQVFKWDGQTRDI
                     AAWNRDHDLITAMKYSVVPVYQEFARQIGEARMSKMLHAFDYGNEDISGNVDSFWLDG
                     GIRISATQQIAFLRKLYHNKLHVSERSQRIVKQAMLTEANGDYIIRAKTGYSTRIEPK
                     IGWWVGWVELDDNVWFFAMNMDMPTSDGLGLRQAITKEVLKQEKIIP"
     gene            complement(1333..1485)
                     /locus_tag="IU325_23655"
                     /pseudo
     CDS             complement(1333..1485)
                     /locus_tag="IU325_23655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
     gene            1861..2826
                     /locus_tag="IU325_23660"
     CDS             1861..2826
                     /locus_tag="IU325_23660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicase"
                     /protein_id="HAP5811957.1"
                     /translation="MRKCAQLELFRERLPRKPYYSDELTTGLRIADVARALGARYIQP
                     NGPTHRHWIVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDG
                     NAAPLRYAAAIEAALREKLGADMGYSGLICKNPLHEHWLVQVWEPRLYDLAWLSDYLD
                     LSSYNGRKSLPEYGLGRNCTLFEKTRLWAYKAIRQGWPDYPEWLAACVDRASGYNAQF
                     EQPLPANEVRHTAKSIAKWTHQHLSPAGFREEQARRGAKGGKVSKGGGRPSNSGKDKS
                     DLLPEVLRLKAQGYTNRDIADDLGEHKKYWGSVSYAAIAGPFPSL"
     gene            complement(2790..4181)
                     /locus_tag="IU325_23665"
     CDS             complement(2790..4181)
                     /locus_tag="IU325_23665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4-like element ISKpn19 family transposase"
                     /protein_id="HAP5811958.1"
                     /translation="MQLTLQIVITDESGSSRTEELMTIQKSGETRNDIGLSVSESKLL
                     LNTVQQSVVQLQADEYTQHHIRCPHCLAARRIKGKQKIRYRTLFGVIPVSGLRVYRCR
                     CEESDTKTVSLLSDWAGDYSHPALKYIETRWASMISYEMTTRLLKDILPVGHSLNAST
                     VRNHLCQVAQRLDAEAEAHSGFLSGCPRDWGNLPRPGKPLVVGIDGGYVRDRDDKKRN
                     FEIIAGKSFSVGAPADTRRFGFVQKDDCHPERRLMTHLSAQGMQANQQIFFLSDGADN
                     LRDLQFGMYPESTHVLDWFHITMRLTVLMQYARGLLVSDPEAGSKVLALLESIKRYLW
                     HGNVVAALEHIDNCVMYCDDPELSYPSLKSLQKHLDEMYTYIRNNKMMIPNYGEMRRY
                     GEPVSTAFVESTINEVIARRMAKKQQMQWSRKGAHYLLQTRTAVLNNELQDKFVCWYP
                     GFQSDGKGPAMAA"
     gene            complement(4218..4790)
                     /locus_tag="IU325_23670"
     CDS             complement(4218..4790)
                     /locus_tag="IU325_23670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP5811959.1"
                     /translation="MQYAYIRVSSADQNTARQEEALSKAGFQPDKICVEHASAKDTNR
                     PGLQELLGQLRPGDTLLVHSIDRLCRNMSDMCAVTTRLRDQGVTLIFLKEQLTFSAGT
                     NNPMQELQLHMMSAFSQFERALLKERQADGIAAKKARGEKTGRPGADIKKIQEIDALR
                     SRGVRLRIACDHAGLGVSTYYKLRHQMNNQ"
     gene            complement(4927..5517)
                     /locus_tag="IU325_23675"
     CDS             complement(4927..5517)
                     /locus_tag="IU325_23675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HAP5811960.1"
                     /translation="MKIYVIKIAVHGVSPMVWRRLRIAADTSLAALHFIFQIVQGWGD
                     DHLHQFHIYGKDYGISYEGGIGFVDNPFRVVIDDFAFDAGDRFTYEYNFFEHWLHDIR
                     VEAIYENSTLKAPFCISGHGMPGATAADEFDKTLAFLEAIVNADDETTVGEIRPFADD
                     LDAVRFNRHKINRQLSRLDLASPVLEPEVIWLGRRR"
     gene            complement(5608..5790)
                     /locus_tag="IU325_23680"
                     /pseudo
     CDS             complement(5608..5790)
                     /locus_tag="IU325_23680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060658.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
     gene            5952..6566
                     /locus_tag="IU325_23685"
     CDS             5952..6566
                     /locus_tag="IU325_23685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014754501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP5811961.1"
                     /translation="MFIGYIRMSKADGSQTNDLQRDALIAAGVDAAHFYEDRASGKRE
                     ERPGLTACLKALRPGDTLVVWKLDRLGRDLRHLINTVHDLTARGTGLKVLTGHGATID
                     TTTAAGKLVFGIFAALAEFERELIAERTTAGLASARARGRNGGRPYKMTPVKLRLAMA
                     SMGQSETKVSTLCQELGITRQTLYRHISPVGQLRADGIKLFNRG"
BASE COUNT         1646 a         1641 c         1698 g         1601 t
ORIGIN      
        1 taatagttaa agcttgtgcc tgattgttgc cacaattggt cagcagtttg ctagggaatg
       61 agagggcaga gaatgataag cttagcactt aatttgtatg gatatattgc attaagcaag
      121 gggacgttat gcgtgtatta gccttatcgg ctgtgttttt ggtggcatcg attatcggaa
      181 tgccagcggt agcaaaggaa tggcaagaaa acaaaagttg gaatgctcac tttactgaac
      241 ataaatcaca gggcgtagtt gtgctctgga atgagaataa gcagcaagga tttaccaata
      301 atcttaaacg ggcgaaccaa gcatttttac ccgcatctac ctttaaaatt cccaatagct
      361 tgatcgccct cgatttgggc gtggttaagg atgaacacca agtctttaag tgggatggac
      421 agacgcgtga tatcgccgct tggaatcgtg accatgactt aattaccgcg atgaagtact
      481 cagttgtgcc tgtttatcaa gaatttgccc gccaaattgg tgaggcacgt atgagtaaaa
      541 tgctgcacgc cttcgattat ggcaatgagg atatctcggg caatgtagac agtttttggc
      601 tcgatggtgg tattcgcatt tcggctaccc agcaaatcgc ttttttacgc aagctgtatc
      661 acaacaagct gcacgtttct gagcgtagtc agcgcatcgt gaaacaagcc atgctgaccg
      721 aagccaatgg cgactatatt attcgggcta aaacgggata ctcgactaga atcgaaccta
      781 agattggctg gtgggttggt tgggttgaac ttgatgataa tgtgtggttt tttgcgatga
      841 atatggatat gcccacatcg gatggtttag ggctgcgcca agccatcaca aaagaagtgc
      901 tcaaacagga gaaaattatt ccctagaagt ggttagcgcg tatttgtgtg aaatagccgt
      961 catataagct gtaaagatat atggacaaaa tgctgatgct cgatgcgctt gtagttaatg
     1021 gctcatactt aatcggtcag gcctaatcga cttaagctcc gcgcgttaac tttcagcctg
     1081 agcattagca tcacattcct aaacgtaaaa agcctagcca atttgctagg cttttttgtt
     1141 atctcaatgg ccatggtcac tcaacatggg ggtgtgaata aaggagtggc gatgtcagta
     1201 aaggtgaatg ccgctgcgat aaagtgccta gcccatcaag gattgtaggc tttcttggct
     1261 gctaaagcgt ttgacgaaaa agtccaagaa ggcgctgata ttggttctcc atcattttca
     1321 tctaccggtt tactataggt caaccaggcc gtccttgccg gcgcttggga cgcagacgat
     1381 ggcatcaaat gcctcgctca aattcgtttc tacagaggcg ctttgggacc gcattcgctt
     1441 tgctttcatg ggggcatctg tcaatgtcag acatgaatcc tccgatatag gttgattttt
     1501 gatctcttca acacttggtg ttgtccgact agagagactt atatggatca gtattcttgc
     1561 actgacaatg gtgccactca atcccaagga tcgacctagt tagaggcgta gatcaagcaa
     1621 ttcaaaacag cgcagaagca gaaggaggtg gaaatatgtt ccagggtgaa aggttacatg
     1681 caactaacgt tgctctctag gatgtgcctg ctgtaacgta ttgtccagaa aagacaaacc
     1741 cccgttatcg cttgcatctt ggcggagctg cgaaaaacgg gggccgtgac gaatcacaag
     1801 agaagtctat cacatggatc atgccaaaca accccacgcc gccaataagc gcggtggtgc
     1861 ttgagaaagt gcgcacagct tgagctgttt cgggagcgcc tgccccggaa accctactat
     1921 tccgacgagc ttacaacggg cctacggatt gcggatgttg cccgcgccct tggtgcgcgt
     1981 tacatccagc cgaacgggcc aacgcaccgc cactggatcg tttttgacgt agaccatgca
     2041 gccgctacgt tatcttggga tgacgtaggc gctccagccc ctaacattac cgtcacgaac
     2101 aaggccaacg gccacgcgca tctgatctac ggactggata cgccgatccg cactgccccg
     2161 gatggaaatg cagcccctct gcggtatgcc gccgccatcg aggccgcgct ccgggagaaa
     2221 ttaggggcgg atatgggcta ctccgggctg atctgcaaga accccctgca tgagcactgg
     2281 ctggtgcagg tatgggaacc ccggctctac gatttagcct ggctatccga ttacctcgat
     2341 ctgagctcct acaatggccg caaatcgctt cctgagtacg ggcttgggcg aaactgtacc
     2401 ctgttcgaga aaacgcgcct gtgggcctat aaagcgatcc ggcagggctg gccggattac
     2461 cccgaatggt tggccgcttg tgttgaccgg gcatcaggat ataacgccca gttcgagcag
     2521 ccgctccccg caaacgaagt ccggcacacg gccaagtcca ttgccaaatg gacgcaccag
     2581 cacctaagcc ccgcagggtt cagagaggag caggcccgga gaggtgcgaa gggcggcaag
     2641 gtatccaagg gagggggaag gccgagcaat agcggtaagg acaagtccga tctattgccg
     2701 gaggttctgc ggcttaaggc tcaggggtac accaaccgcg atatagcgga cgatcttgga
     2761 gagcataaaa aatattgggg cagcgtgagc tatgctgcca tcgctggtcc cttcccgtca
     2821 ctttgaaaac ccggatacca gcagacaaat ttatcctgca gttcgttatt caaaacggcg
     2881 gtccgggtct gtaacaaata atgagcccct tttctgctcc actgcatctg ctgctttttg
     2941 gccattcgtc tggcgatcac ttcattgatc gtggattcca caaacgcggt tgataccggc
     3001 tctccgtacc gacgcatttc gccgtagttt gggatcatca tcttattatt ccggatgtag
     3061 gtatacattt catccagatg tttttgcagg gatttcaggc taggatagct gagttcaggg
     3121 tcatcgcaat acataacgca attatcaata tgttccagcg cagcgacaac attgccatgc
     3181 cagagatatc gtttaatgct ttccagcagt gccagaactt tactccccgc ctctggatct
     3241 gataccagca gtccccgggc atattgcatg agcaccgtca gcctcatggt gatatgaaac
     3301 cagtccagca catgcgttga ctcagggtac ataccgaact ggaggtccct gagattatcc
     3361 gcgccgtcgg acagaaaaaa tatctgctgg ttggcctgca ttccctgcgc tgaaaggtgg
     3421 gtcatgagcc tgcgttccgg atgacagtca tccttctgaa caaaaccgaa gcggcgggtg
     3481 tcagcaggcg caccaacgga aaaagacttc ccggcaataa tttcaaaatt acgctttttg
     3541 tcatcccgat cgcgaacata accaccatct atacccacca caagcggttt tcctggcctg
     3601 ggcaggttcc cccagtcgcg ggggcagccg gaaagaaaac ctgaatgagc ttcagcctca
     3661 gcgtcaagac gctgcgccac ctggcataaa tgattcctca ccgttgaggc attcaggctg
     3721 tggcccaccg gcagaatatc tttcagcaga cgagtcgtca tttcatagga gatcatcgag
     3781 gcccagcggg tttcaatata tttcagtgcc gggtgagaat aatcgccggc ccagtcgctg
     3841 agcaaactga ctgtctttgt atcactctct tcacaacggc accggtacac ccgaagcccg
     3901 gataccggaa taacgccaaa cagcgtccgg taccgtattt tctgtttgcc tttgattctg
     3961 cgcgcagcaa ggcagtgagg gcaccggata tggtgttgag tatattcgtc tgcctgcagc
     4021 tggaccaccg actgctggac cgtattcagc aacagcttgg attctgacac cgataatccg
     4081 atgtcgttcc gggtctcccc tgatttctgg attgtcatca gttcctccgt tcgactggag
     4141 cctgattcat cggtgatgac tatctggagc gtcagttgca tcattgcctc ctgctgtggt
     4201 ggatgactat ctcccggcta ctggttgttc atctggtgac gaagcttata atatgtggaa
     4261 acgccaaggc ccgcatggtc gcaggcgatc ctgagcctga cccctctgct tctgagtgcg
     4321 tcaatttcct ggatcttttt gatatcggcg ccggggcgcc ctgttttttc accccgtgct
     4381 tttttcgcgg cgatgccgtc ggcctgcctt tccttcagta aggctcgttc aaactggctg
     4441 aatgccgaca tcatgtgcag ctgcagttcc tgcatcggat tgtttgtgcc ggcgctgaac
     4501 gtcagctgct ctttcagaaa aataagggta acgccctggt cacgaagccg ggtggtgaca
     4561 gcgcacatat ccgacatatt ccggcaaagg cgatcgatgg aatgaaccag aagcgtatca
     4621 ccagggcgta attgccccag gagttcctgt aaccccgggc ggtttgtgtc cttcgcgctg
     4681 gcatgctcaa cacaaatttt atcaggctga aaacccgcct ttgacaatgc ctcttcctga
     4741 cgggccgtat tctgatcggc cgagcttacc ctgatgtagg catattgcac gcaaaacctc
     4801 cggaaggtct ttaaacatca tagagattta aagaataatc cataaacact gatttaggga
     4861 ggtttataga cattttttcg gggatctgag aggcatgctg aaaacgtata cctttataga
     4921 catcagtcag cgacgacggc ccagccagat aacttcaggc tccagtaccg gagatgcaag
     4981 gtcgagcctg ctcagttgcc ggttgatttt gtggcggttg aacctgacag catccaggtc
     5041 gtcagcaaaa gggcggatct cgccgaccgt tgtttcatca tccgcattaa cgatagcttc
     5101 aagaaacgcc agggttttat cgaactcatc cgcagctgtg gctccgggca taccatggcc
     5161 gcttatacaa aacggcgctt tcagcgtaga gttttcataa atggcctcaa cacgaatgtc
     5221 atgaagccag tgctcaaaga aattgtattc ataggtaaag cgatcgccag catcaaaagc
     5281 aaaatcatca atcacgaccc ggaacggatt atcgacaaaa ccgatgcctc cttcgtagga
     5341 gataccgtaa tctttgccat aaatgtgaaa ttgatggaga tggtcgtccc cccagccctg
     5401 cactatctgg aaaatgaagt gaagcgcggc cagtgacgtg tcggcagcaa ttctcagccg
     5461 acgccagacc atcggactga ctccatgaac tgctattttg atgacgtaga tcttcatagt
     5521 cagcaggtat tgttatgaaa tgatggtggt tattttaacc cctgtagatg gcatcattcg
     5581 atggctgccc cgaggttttt catgctctcc gatctggatg atgcaataaa tttgagcgtg
     5641 aagcccccgg cgactgcagg ccagcgccag ttcatattca ttcagcgcca gaaactccag
     5701 ccgctgtgca tcatcgaaat caggtagacc atagagcgct tcctgctcgg cgtccgaaag
     5761 aatactgagg agcttatttt tagtaggcat ctctgatcct tatgcctatt taaacgcgct
     5821 tgtcacatta tcattaacat agaaccttta cgtgtcataa tttaaattac gggtaatgtg
     5881 actaccgttg acagattatg gtacccaaat cagaatgtca cataatcgaa cgtatatgtg
     5941 acagaggaaa tatgttcatc ggatatattc ggatgtcaaa agcggatggt tcgcagacga
     6001 atgatttaca acgtgatgca ctgatcgccg caggtgttga tgccgcacat ttttatgagg
     6061 accgggcatc gggcaaacga gaagagcgtc ctggacttac agcctgtctg aaagcactac
     6121 gacccggaga cacgctggtc gtatggaagc tggaccgcct cggtcgtgat cttcgtcatc
     6181 tcattaatac agtgcacgac ctgaccgcga gggggaccgg tctgaaagtg ctgaccggtc
     6241 acggggcgac tatcgacaca acaacggccg ctggcaagct tgtcttcggt atctttgccg
     6301 cactggcgga gtttgagcgc gaactgattg ctgaaagaac gactgcaggt ctggcatcag
     6361 caagagcccg tgggcggaac ggcggccgac cttataaaat gacgccagtc aaactgcgac
     6421 tggcaatggc atcaatggga caatcagaaa caaaggtcag tacgctatgc caggaacttg
     6481 ggataacccg gcaaactttg tacaggcata tttcccctgt tggtcaactg cgggcggacg
     6541 ggatcaagct gttcaaccgt ggataatttg aacggacgga aaaatc
//
LOCUS       DACBVI010000436         5260 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.guided.236, whole genome shotgun sequence.
ACCESSION   DACBVI010000436 DACBVI010000000
VERSION     DACBVI010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.guided.236"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            <1..237
                     /locus_tag="IU325_23690"
     CDS             <1..237
                     /locus_tag="IU325_23690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP5811962.1"
                     /translation="PLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVGMPDLHP
                     GRGYPIGAALFSVGRFYPARRRGNGAGNRNGPLL"
     gene            206..537
                     /locus_tag="IU325_23695"
                     /pseudo
     CDS             206..537
                     /locus_tag="IU325_23695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(594..2051)
                     /gene="pepD"
                     /locus_tag="IU325_23700"
     CDS             complement(594..2051)
                     /gene="pepD"
                     /locus_tag="IU325_23700"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAP5811963.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2312..2770
                     /locus_tag="IU325_23705"
     CDS             2312..2770
                     /locus_tag="IU325_23705"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP5811964.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2862..4106
                     /gene="frsA"
                     /locus_tag="IU325_23710"
     CDS             2862..4106
                     /gene="frsA"
                     /locus_tag="IU325_23710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAP5811965.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            4164..4565
                     /locus_tag="IU325_23715"
     CDS             4164..4565
                     /locus_tag="IU325_23715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAP5811966.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(4604..>5260)
                     /locus_tag="IU325_23720"
     CDS             complement(4604..>5260)
                     /locus_tag="IU325_23720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HAP5811967.1"
                     /translation="GDSSAQTDNFMTKRASGLATYRNTDFFGVIDGLNLTLQYQGKNE
                     NRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNLQSRGTGKRAEAWATGL
                     KYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQFDFGLRPSLGYVLSKGKD
                     IEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLNINNDDIVAVGMTYQF
                     "
BASE COUNT         1264 a         1305 c         1361 g         1330 t
ORIGIN      
        1 cccttatccg atgcggtatt taccatcgca tctgatgacc tgtggatcga gagtttagcg
       61 atccaacaat tacacaccac ggcaaattta cccaacatgc agcgcgtagt agggatgcca
      121 gatttacacc ccggacgcgg ctacccgatt ggcgcagcgt tattctctgt tggtcgtttt
      181 tacccggcaa gacgtcgcgg taacggtgct ggaaacagaa acgggccgct gctctgacac
      241 gctgcgttcg gcgctgattt ctctggatgg cgataacgca tgggcattaa gcgaaagttg
      301 gtgcggcact attcagtgga tttgtccgag tccgtatcgg cctcatcatg ggcgcaaaaa
      361 cttgtttctg ggcatgctaa caagcggctg gcgcgattgc tgattgcctg gaagctggaa
      421 caacagcaac aggaaaatag cgcggtgctg aaatcgcagc ggcgaatgtt ccatcaccag
      481 attgaacgtg gcaacccgag acggacattt acagggatgg cttttatcga aggataatga
      541 aggatgaaac tgccggaagg cgattaaacg ccatccggca gcaaatcaaa taattacttc
      601 gccggaattt ctttcagcag ttcagtcagc agtgtccagt aatgacctac gctttcgatg
      661 tgaacttgct catccggaga gtgtggaccg gtgattgttg gcccgataga aaccatgtcc
      721 atttccggat acggtttttt aaacagacca cattccaggc ccgcgtgaat aatctggatg
      781 ttcggcgtct tgttgaacag tcgctgatag gtttcacgta ccagatgcat caccggagaa
      841 ttagcgtccg gctgccagcc aggatatgcg cctttcgctt cggttttcgc gccagccagt
      901 ttacccagcg aatccagcat gctcaccacg tagtctttac cgctgtcgat cagagaacgg
      961 atcaggcagt gaatttctac gttattgtca gtcatggtca ccacaccgac gttcagggag
     1021 gtttcgacca caccttttgc cacgtcggag ttgcggatca caccgttcgg ggtggcgttc
     1081 agcagacgaa taaaggtatc gcgagatttc gcaatcaggg cagctttatc gttcgcaaca
     1141 gagtccagca gcaatgccag atttttctct ttctctgcca gctcgttttt caggatttcc
     1201 tgataggtat tcaccagaga tttcagggcg tcgactttat cagctgcgac agcaatggtc
     1261 gcaaaggctt cacgcgggat ggcgttacgc agtgtgccac cgttgaaatc gataaggcgc
     1321 aggtccagct cttctgcatg acccgccagg aagcgcacca gcagtttgtt ggcattaccc
     1381 aggccaacgt ggatttcacc gccggagtga ccgcctttta gaccttttaa ggttaacttg
     1441 aaggtttcaa aaccagctgg aactgcttca cgatctaaat gcaggttgga ggtgaagtcg
     1501 ataccccccg cgcaacccat gtagatttca ccttcctctt cggagtcggt gttaatcaga
     1561 atatcagcct gcaaccagtt gctctgtaag ccaaaagcac catccatacc ggcttcttcg
     1621 gtcatggtca gcagcacttc cagcgggccg tgaaccacgt tttcgtcagc cagaaccgcc
     1681 agcgcagacg ccataccaat gccgttatcc gcacccagcg tggtgccgcg cgctttaacc
     1741 cattcgccat caatataagg ctggatagga tctttagtga agtcatgcac ggtgtcgtta
     1801 tttttctgtg gcaccatatc gaggtgggcc tgcaagacga ccggtttacg attttccata
     1861 cctgcggtag caggtttacg aatcaggata ttacctacct gatcgcgttc gacatggaaa
     1921 cctttctctt ttgcccaacc aacaatgtat tcagcgagtt gctcttcatg ataggacggg
     1981 tgaggaatag aacagatttt ggcaaaaata tcccacagcg gctgtggaga taattgagac
     2041 agttcagaca cgttaagtct ccttgtcgat cacccgcaaa acagtattgc aggtcacagg
     2101 gttagcagaa aatgttgtca acacaagaca ggcttgcgag atatgtttga gaataccact
     2161 ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt
     2221 ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta
     2281 agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga
     2341 catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa
     2401 aggcattatt gccgtaagcc gtggcggtct ggtaccgggt gcgttactgg cgcgtgaact
     2461 gggtattcgt catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgcga
     2521 gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct
     2581 ggtggatacc ggtggcactg cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt
     2641 caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg ttgatatccc
     2701 gcaagatacc tggatcgaac aaccgtggga tatgggcgtc gtattcgtcc cgccaatctc
     2761 cggtcgctaa tcttttcaac gcctggcact gccgggcgtt gttcttttta acttcaggcg
     2821 ggttacaata gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga
     2881 gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct
     2941 ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga tggtaaaacc atccctcact
     3001 ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg cattgaccca cgtgaaatcc
     3061 tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg taccgacgat gatttatacg
     3121 atacggtgat tggctaccgt ggtggcaact ggatttatga gtgggccacc caggcgatgg
     3181 tgtggcaaca aaaagcctgt gcggaagaag atccgcaact cagtggtcgt cactggctgc
     3241 atgcggctac gttgtacaac attgccgcct atcctcatct gaaaggagat gacctggccg
     3301 agcaagcgca ggctttgtca aaccgcgcct atgaagaggc cgctcagcgt ctgccgggca
     3361 ccatgcggca gatggagttt accgtacccg gcggtgcgcc catcaccggc tttttgcata
     3421 tgccgaaagg cgatggcccg ttcccgacag tattaatgtg tggtggtctg gatgcgatgc
     3481 agacggacta ttacagcctg tatgaacgtt attttgcgcc gcgcggcatt gcgatgctga
     3541 ctattgatat gccgtcggtg ggcttttctt caaaatggaa gctcacccag gactccagcc
     3601 tgttgcatca gcacgtctta aaggcgctgc ctaacgtacc gtgggtagat cacactcgcg
     3661 tcgcggcctt tggtttccgt ttcggcgcta acgttgccgt gcgtctggca taccttgaat
     3721 cgccgcgtct gaaagcggtt gcctgtcttg gtccggtagt tcataccctg ttgagtgatt
     3781 ttaagtgcca gcaacaggtg ccggaaatgt atcttgacgt tctggcgagc cgtttgggga
     3841 tgcatgatgc ttccgacgaa gcgttgcgcg tggagctgaa tcgctattca ttaaaagtgc
     3901 aaggattgct gggacgtcgc tgcccaacgc caatgttatc aggctactgg aagaacgatc
     3961 cgttcagccc ggaagaggac tcacgcttaa tcacctcatc atctgctgac ggtaaattgt
     4021 tagagatccc atttaacccg gtgtatcgga attttgacaa aggtcttcag gaaataacag
     4081 actggattga aaaacgattg tgttaaaaat ttgctaaatt ttgccaattt ggtaaaacag
     4141 ttgcatcaca acaggagatt gcaatgacgt taccgagtgg acacccgaag agcagattga
     4201 tcaaaaaatt taccgcacta ggcccgtata ttcgtgaagg taagtgcgaa gataatcgat
     4261 tctttttcga ttgtctggct gtatgcgtca acgtgaaacc ggcaccggaa gtgcgtgagt
     4321 tctggggctg gtggatggaa ctggaagcgc aggaatcccg ttttacctac agttaccagt
     4381 ttggtctgtt cgataaagca ggcgactgga cgagtgttca gataaaagat gccgaagtgg
     4441 ttgaacgact ggagcacacc ctgcgtgaat ttcacgagaa gctgcgtgag ctgctggcga
     4501 cgctgaatct gaagctggaa ccggcggatg attttcgtga tgaaccggtg aagttaacgg
     4561 cgtgagtgaa atgtgccgga tgcatcacat ccggcaatat ccattaaaac tgataggtca
     4621 tgccaaccgc gacaatatca tcattattga tgttcagctt attatcgcta tccagttggt
     4681 tgattttata atcaacaaac gctgacatat ttttgttgaa ataatatgta gcaccgacgt
     4741 cgatataatt gaccagatct tcatcaccga tgccttcaat atctttccct ttcgataaga
     4801 cataacccag cgacggacgc agaccaaagt caaactggta ttgggcgacc gcttcaaagt
     4861 tctgtgtctt attggcaaag ccgccagtta ttggcgtcat tttgcgtgtt tcagaataga
     4921 aagttgccag ataaatatta ttggcatcgt atttcagacc tgttgcccat gcttctgcac
     4981 gcttgcctgt gccacggctt tgcaggttct gctcgttagt gcgatcggag ttggtatagg
     5041 ccccactgat ggcgaaatcg ctgccaccaa agtcatatgt caatgacgta ccgaagccat
     5101 cgccgttttg ctttttaacg tcgcggtttt cgtttttccc ttgatattgc agggttaagt
     5161 tcaggccatc gataacgccg aagaagtcgg tgttccgata cgtcgccaga ccgctggcgc
     5221 gtttggtcat aaagttgtcg gtctgcgccg aggagtcgcc
//
LOCUS       DACBVI010000437         3565 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.guided.262, whole genome shotgun sequence.
ACCESSION   DACBVI010000437 DACBVI010000000
VERSION     DACBVI010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.guided.262"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            49..924
                     /locus_tag="IU325_23725"
     CDS             49..924
                     /locus_tag="IU325_23725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase
                     CTX-M-15"
                     /protein_id="HAP5811968.1"
                     /translation="MVKKSLRQFTLMATATVTLLLGSVPLYAQTADVQQKLAELERQS
                     GGRLGVALINTADNSQILYRADERFAMCSTSKVMAAAAVLKKSESEPNLLNQRVEIKK
                     SDLVNYNPIAEKHVNGTMSLAELSAAALQYSDNVAMNKLIAHVGGPASVTAFARQLGD
                     ETFRLDRTEPTLNTAIPGDPRDTTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNT
                     TGAASIQAGLPASWVVGDKTGSGGYGTTNDIAVIWPKDRAPLILVTYFTQPQPKAESR
                     RDVLASAAKIVTDGL"
     gene            complement(971..1303)
                     /locus_tag="IU325_23730"
     CDS             complement(971..1303)
                     /locus_tag="IU325_23730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin fold metalloprotein, WbuC family"
                     /protein_id="HAP5811969.1"
                     /translation="MRPHRHPHTFELLLPLRGRFVVLNFDDRGTVTHRAILGETCTVL
                     EMAAGTWHAVLSLDTGGIIFEVKHGGYQPVAADDYAHWAPAEGEPGTTELMAWYAQAQ
                     VGDSTFAV"
     gene            1317..3565
                     /locus_tag="IU325_23735"
                     /pseudo
     CDS             1317..3565
                     /locus_tag="IU325_23735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496645.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
BASE COUNT          852 a          918 c          974 g          821 t
ORIGIN      
        1 gactattcat gttgttgtta tttcgtatct tccagaataa ggaatcccat ggttaaaaaa
       61 tcactgcgcc agttcacgct gatggcgacg gcaaccgtca cgctgttgtt aggaagtgtg
      121 ccgctgtatg cgcaaacggc ggacgtacag caaaaacttg ccgaattaga gcggcagtcg
      181 ggaggcagac tgggtgtggc attgattaac acagcagata attcgcaaat actttatcgt
      241 gctgatgagc gctttgcgat gtgcagcacc agtaaagtga tggccgcggc cgcggtgctg
      301 aagaaaagtg aaagcgaacc gaatctgtta aatcagcgag ttgagatcaa aaaatctgac
      361 cttgttaact ataatccgat tgcggaaaag cacgtcaatg ggacgatgtc actggctgag
      421 cttagcgcgg ccgcgctaca gtacagcgat aacgtggcga tgaataagct gattgctcac
      481 gttggcggcc cggctagcgt caccgcgttc gcccgacagc tgggagacga aacgttccgt
      541 ctcgaccgta ccgagccgac gttaaacacc gccattccgg gcgatccgcg tgataccact
      601 tcacctcggg caatggcgca aactctgcgg aatctgacgc tgggtaaagc attgggcgac
      661 agccaacggg cgcagctggt gacatggatg aaaggcaata ccaccggtgc agcgagcatt
      721 caggctggac tgcctgcttc ctgggttgtg ggggataaaa ccggcagcgg tggctatggc
      781 accaccaacg atatcgcggt gatctggcca aaagatcgtg cgccgctgat tctggtcact
      841 tacttcaccc agcctcaacc taaggcagaa agccgtcgcg atgtattagc gtcggcggct
      901 aaaatcgtca ccgacggttt gtaatagcgg aaacggaatg gggaaactca ttccgttttt
      961 gtttatcgcc ttagacggca aaagtgctgt cgcccacctg cgcttgcgca taccaggcca
     1021 taagctccgt ggttcctggt tctccttccg ctggagccca gtgcgcatag tcatcggcag
     1081 ccacgggttg atagccaccg tgttttactt caaaaattat gccaccggta tccagcgaca
     1141 gcacggcatg ccaggttcct gcggccatct ccagcaccgt acaggtttcc cccaatatcg
     1201 cccgatgggt gacggtaccc cgatcgtcaa aattcagcac cacgaaacga ccccttaatg
     1261 gcaacagtag ctcgaaggtg tgagggtgtc ggtgcgggcg cacgtaggtc ccaggtcata
     1321 ttccttccgg cgtccggcat tttaccgcca gacagctcgg gattcgtgat atcaccgttc
     1381 ttgcagaata cggtcagagg gaaaataccc gccgtgagca tgcagcgctg atacgtcagc
     1441 actatcagta tcgtgaattt gcctggccct ggacatttcg ccttacccgt cttttatata
     1501 cccggagctg gataagcaac gaacgtcctg gcctgctttt cgatctggcg acagggtggc
     1561 ttatgcaaca tcgtattatt ctccccggag ccactacgct gacccggttg atttcagagg
     1621 taagggaaaa ggcgacgttg cgcctgtgga acaaactggc actgataccg tcagccgaac
     1681 agcgttcaca gctggagatg ctgctggggc caactgattg cagccgcctg tctttactgg
     1741 aatcactgaa aaagggccct gtgaccatca gtggtccggc gtttaatgaa gcaattgaac
     1801 gctggaaaac tctgaacgat tttggcctgc atgctgaaaa cctgagtaca ctcccggctg
     1861 tgcgcctgaa aaatctcgca cgttatgctg gtatgacttc ggtgttcaat attgccagga
     1921 tgtcaccgca gaaaaggatg gcggttctgg ttgcctttgt ccttgcatgg gaaacgctgg
     1981 cgctggatga tgcattggac gttctggacg ccatgctggc cgttatcatc cgtgacgcca
     2041 gaaagattgg gcagaaaaaa cggctccgct cgctgaagga tctggataaa tctgcattgg
     2101 cgctcgccag cgcatgttcg tacctgctga aagaagaaac accggacgaa tcgattcgtg
     2161 ctgaggtgtt cagctacatc ccaaggcaaa agctggctga aatcatcacg cttgtccgtg
     2221 aaattgcccg gccctcagac gataattttc atgaagaaat ggtggagcag tacgggcgcg
     2281 ttcgtcgttt cctgccccat ctgctgaata ccgttaaatt ttcatccgca cctgccgggg
     2341 ttaccactct gaatgcctgt gactacctca gccgggagtt cagctcacgg cggcagtttt
     2401 ttgacgacgc accaacggaa attatcagtc ggtcatggaa acggctggtg attaacaagg
     2461 aaaaacatat cacccgcagg ggatacacgc tctgctttct cagtaaactg caggatagtc
     2521 tgaggcggag ggatgtctac gttaccggca gtaaccggtg gggagatcct cgtgcaagat
     2581 tactacaggg tgctgactgg caggcaaacc ggattaaggt ttatcgttct ttggggcacc
     2641 cgacagaccc gcaggaagca ataaaatctc tgggtcatca gcttgatagt cgttacagac
     2701 aggttgctgc acgtctttgc gaaaatgagg ctgtcgaact cgatgtttct ggcccgaagc
     2761 cccggttgac aatttctccc ctcgccagtc ttgatgagcc ggacagtctg aaacgactga
     2821 gcaaaatgat cagtgatcta ctccctccgg tggatttaac ggagttgctg ctcgaaatta
     2881 acgcccatac cggatttgct gatgagtttt tccatgctag tgaagccagt gccagagttg
     2941 atgatctgcc cgtcagcatc agcgccgtgc tgatggctga agcctgcaat atcggtctgg
     3001 aaccactgat cagatcaaat gttcctgcac tgacccgaca ccggctgaac tggacaaaag
     3061 cgaactatct gcgggctgaa actatcacca gcgctaatgc cagactggtt gattttcagg
     3121 caacgctgcc actggcacag atatggggtg gaggagaagt ggcatctgca gatggaatgc
     3181 gctttgttac gccagtcaga acaatcaatg ccggaccgaa ccgcaaatac tttggtaata
     3241 acagagggat cacctggtac aactttgtgt ccgatcagta ttccggcttt catggcatcg
     3301 ttataccggg gacgctgagg gactctatct ttgtgctgga aggtcttctg gaacaggaga
     3361 ccgggctgaa tccaaccgaa attatgaccg atacagcagg tgccagcgaa cttgtctttg
     3421 gccttttctg gctgctggga taccagtttt ctccacgcct ggctgatgcc ggtgcttcgg
     3481 ttttctggcg aatggaccat gatgccgact atggcgtgct gaatgatatt gccagagggc
     3541 aatcagatcc ccgaaaaata gtcct
//
LOCUS       DACBVI010000438         1356 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain M94949
            SAMN12529908-rid8905933.guided.312, whole genome shotgun sequence.
ACCESSION   DACBVI010000438 DACBVI010000000
VERSION     DACBVI010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12529908
            Sequence Read Archive: SRR9924668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-JAN-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000664679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000664679.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2020 08:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.10
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,655
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 5, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12529908-rid8905933.guided.312"
                     /strain="M94949"
                     /isolation_source="BLOOD CULTURE"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Brisbane"
                     /collection_date="2017"
     gene            402..1058
                     /locus_tag="IU325_23740"
     CDS             402..1058
                     /locus_tag="IU325_23740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrS1"
                     /protein_id="HAP5811970.1"
                     /translation="METYNHTYRHHNFSHKDLSDLTFTACTFIRSDFRRANLRDTTFV
                     NCKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIEFRACDLKGANFSRT
                     NFAHQVSNRMYFCSAFISGCNLSYANMERVCLEKCELFENRWIGTNLAGASLKESDLS
                     RGVFSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIAAWQQELILEALGIVVYPD
                     "
BASE COUNT          381 a          287 c          292 g          396 t
ORIGIN      
        1 caaccatctt atcgttctga ctggtattaa acatagttcc aagactacct cttattttaa
       61 gagagtcgaa gtgtctggtg atctatgggg ccagtctagc cctcttccaa gtgtagggag
      121 atatagtcaa agtttgcttc tccgccgatg agtccgccct gcgtcggata gtccgatttt
      181 ttaccggcga agcacggctc tcctaaccct atctaaagcg aataactttt ttcaacaggg
      241 ccgcgtgcca atgcaagagc gataggcgct ctacgcgcca atttgaccac ttaaaacagg
      301 taaattgagg ggttgtaatg tgttgatgta acaggctttt attttaatgt cttggcatat
      361 gtataatggt agtctagccc tcctttcaac aaggagtact catggaaacc tacaatcata
      421 catatcggca ccacaacttt tcacataaag acttaagtga tctcaccttc accgcttgca
      481 cattcattcg cagcgacttt cgacgtgcta acttgcgtga tacgacattc gtcaactgca
      541 agttcattga acagggtgat atcgaaggct gccactttga tgtcgcagat cttcgtgatg
      601 caagtttcca acaatgccaa cttgcgatgg caaacttcag taatgccaat tgctacggta
      661 tagagttccg tgcgtgtgat ttaaaaggtg ccaacttttc ccgaacaaac tttgcccatc
      721 aagtgagtaa tcgtatgtac ttttgctcag catttatttc tggatgtaat ctttcctatg
      781 ccaatatgga gagggtttgt ttagaaaaat gtgagttgtt tgaaaatcgc tggataggaa
      841 cgaacctagc gggtgcatca ctgaaagagt cagacttaag tcgaggtgtt ttttccgaag
      901 atgtctgggg gcaatttagc ctacagggtg ccaatttatg ccacgccgaa ctcgacggtt
      961 tagatccccg caaagtcgat acatcaggta tcaaaattgc agcctggcag caagaactga
     1021 ttctcgaagc actgggtatt gttgtttatc ctgactaatt gctttgatgt gtgattttaa
     1081 acgctcaaat ttataaaacg aataatattg tcaaccttgc aataatagac atagggacaa
     1141 ccatgtataa atttttaccc tcaggcgtct tcacaataga gccatatagc agtgggtcac
     1201 ttagtgggct taatttttcc attaaagata atattaatat tgctcagtat aaaacatcat
     1261 atggtagccc atcttggcaa gtaaacataa ggctgccatt tataaggcac tgttgcaaaa
     1321 atgtggaggt gataggggta ctacgccgga acccca
//
